LOCUS       DAOPTR010000001        13831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_3741_length_13831_cov_3.367741, whole genome shotgun sequence.
ACCESSION   DAOPTR010000001 DAOPTR010000000
VERSION     DAOPTR010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 13831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13831
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3741_length_13831_cov_3.367741"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PKI05_00005"
     CDS             complement(<1..251)
                     /locus_tag="PKI05_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HOA50603.1"
                     /translation="MPYDVVVCVKQVPDTANVTAEAMKEDGTVNRAALPAIFNPEDLN
                     ALEVALEIRDRWGGTVTVISMGPPKAAEVLRDSLYRGADR"
     gene            complement(803..2269)
                     /locus_tag="PKI05_00010"
     CDS             complement(803..2269)
                     /locus_tag="PKI05_00010"
                     /inference="COORDINATES: protein motif:HMM:NF021041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HOA50604.1"
                     /translation="MLRRDFCRFALLAGGWASLHQVPRLSAANEAGDAALRRSAEEAL
                     RRAVTFFDQEVSIEGGYVWAYAEDLTLREGEGKATPQTAWVQPPGTPSVGMALLQAYH
                     LTKAPLCLQAATRTAHALVKGQLVSGGWDYRIEFAPQARSRYRYRKPPTGGEKAANSS
                     TLDDDNTQSALRFLMMYDRETGMKDEVVHEAVTYGLDALLAVQRPNGGWPQRFVAPPD
                     PKEYPDVPASYPDDWPRQWPNRPYGDCYTINDNTQADALATVLLAWRIYGDEKYLVSA
                     RRAGEFLLKAQMPDPQPAWAQQYDAHMQPCWARKFEPPAITGLESQGVMRALITLFHE
                     TGEKRFLEPIPRAIEYLRRSLLPDGRLARFYELKTNQPIYFTRDYRLTTSDADMPTHY
                     AFKVGNELDAIQRAYEEALRSGPRPRLGRLPTAPRLSASLAAQARRVIEQLDERGRWV
                     ESGRLKTAPADARVTRIITTRTFIQNLRILANYLAASS"
     gene            complement(2325..>3504)
                     /locus_tag="PKI05_00015"
     CDS             complement(2325..>3504)
                     /locus_tag="PKI05_00015"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOA50605.1"
                     /translation="LRHYLDSDFRAAGMSREQYAALFEKPGSPQEKWQILAPFWPAVK
                     NTGYGQAVRIAMREIYGIDDLSEKTVDKVREQYQATRRPGFYRRILCDLAKIDSCQVN
                     LGPYHESKQPLLLLQDISIVGMFAGPDIRAFGRPTGIEVKTLTDWYRVIDWWFDRYAK
                     YAVAVKSQNAYSRNIDYEKVPQERAEPLFAKRLNGESLSAEEQKALEDHLFWYAVGKA
                     TEHHLPVKLHTGYYAGDNRMPLSRLRLNAGSACDLCRMSPETTFVFMHICYPYYEELI
                     AVAKQYTNAYVDMCWSWIINPVAAKDFLKKFLVTAPANKIFTFGGDYRPVEPVLGHAL
                     IARQGIALALSELVEEGWLSLDDALELIDPIMHGNARRVFQVEEKKQNLRSARWFREG
                     "
     assembly_gap    3505..3514
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3515..3755)
                     /locus_tag="PKI05_00020"
     CDS             complement(<3515..3755)
                     /locus_tag="PKI05_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50606.1"
                     /translation="MSHFSRRETLKLLGTVGMSGMMAAGSACALPSVEAPSIPLTSDV
                     RRHIFDRVWKTPLIDSHEHLPDEDDCLRGPGGTPQD"
     gene            complement(4810..5883)
                     /gene="folK"
                     /locus_tag="PKI05_00025"
     CDS             complement(4810..5883)
                     /gene="folK"
                     /locus_tag="PKI05_00025"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOA50607.1"
                     /translation="MTDLASSAPRLVAIGMGSNLGDSWAILREACKALSELPATQLRA
                     TSRIRLTQPIRVSDQGSPFFNAVALLETALPPRELLDGIMAIEQRFGRERNRHWASRT
                     LDLDILLDEAGPFYDQRLLIPHPRLGSRLFFLASLEELLPDWRHPWCGLTVRQMVEIL
                     STRPPYCVLIWETFLPTHAASSLTTHSGEIQAGEADWAALWQACVAANKVFQAGCPHL
                     EDSGGSVSAGVHLTHPPESCIFNAEEVQHRLAMGQSLAEIVSFAWLEHRKQVRDGRET
                     PPPRVMILGPRAKDLLWPHEANASDRPLVSPSFELANLSWLRDQATPVVFSEANDPRT
                     WAEHIIAAIDASAEIGHVLGDLG"
     gene            complement(5937..8147)
                     /locus_tag="PKI05_00030"
     CDS             complement(5937..8147)
                     /locus_tag="PKI05_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOA50608.1"
                     /translation="MDINSLFFAFLGGAAWLYVLMVIVGLGLIIFVHELGHFVVAKLC
                     GVKCEKFYLGFDFFGLRLFRFRWGETEYGIGIIPLGGYVKMLGQEDNPARLREEFERA
                     KAAGQEGAKSSTGESLANMEEALYNPRSYLAKSVPQRMAIISAGVAMNLLFAFIAAVL
                     AYFIGVQEIPCGVGQLAPGEGAWRAGILPGDKILEINGQKTHRFQDLQQSISLARRGQ
                     VVTMRIERPTPNGLKVLDLELMPDRIRVVPTIGVTNPLLPILAEPPCYPGSAAAEVIT
                     SLPPGATVKAIHGEPVTGFADIARSWAKSWGQPVELTLEKKAASGTPLEPQKVVLQPT
                     PVRDLGLIFKVGRIVAVRAGSPAEVAGVLAGDELMSLTCPDNVNWTGNFMRLPYALQK
                     ALRDRPEGIPLQLTVKRSNGSEKAGVALVAMRSATAADKNSSNEGTPLKLDVVAKTAE
                     FFADLNTGDSPFEIPQLGIAVEILPQIEAVEPHSPAAAAGLQPGDELISVTAIPPNLD
                     PESKIGELARHFSMTKFTVRFDQPRGEEKGTWPFFFRELQRMPTGTEVDLVVRRPQPD
                     PAQEYQEVKVHLVPRVADDWFLAERDLLFEAMTFDLRAAGLGEAITMGAAETWASVTL
                     IYRVLNSLGRGDVSPRALVGPIGLVQAAYRVASQGLGRFLLFLTLLSANLAVINFLPI
                     PVLDGGHMAFLIYEGIRGKPPSETVYVGLSYLGLAIILGIMLWVFGLDLGFISR"
     gene            complement(8138..9340)
                     /gene="dxr"
                     /locus_tag="PKI05_00035"
     CDS             complement(8138..9340)
                     /gene="dxr"
                     /locus_tag="PKI05_00035"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOA50609.1"
                     /translation="MAAQVKSQESTLSESWGNRTKTIAVLGSTGSIGRSALEVISASQ
                     GALRAIILVARSNTQLLLQQAQEQRPRLVIVTDPKAAAAHSWRDLPLETELRVGYEAV
                     LEAVSADDVDMVHSAFVGIAGLRATWAALEAGKTVALANKESLVVAGSLITQLARKRK
                     AQILPVDSEHSAIFQALHGGQEREVRRIILTASGGPFRSRKLTELASVTVEEALAHPT
                     WKMGKKITVDSATLMNKALEIIEARWLFGVEADQITVVIHPQSIVHSMVEFVDGSVIA
                     QLSPPDMRLPIQYALYFPERRPGIARKIDWATAMDLRFEPPDLERFPAIRLGLEAARI
                     GGTAGAVLNAANEAAVECFLQGRLRFDQIVPVCAKVLEAHPFDPSPDLDAVFGLDAWA
                     RKEVERWI"
     gene            complement(9419..10726)
                     /locus_tag="PKI05_00040"
     CDS             complement(9419..10726)
                     /locus_tag="PKI05_00040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOA50610.1"
                     /translation="MPRRNLQILVLAIIICLLCAYRSSRYTRVLTYALEQIQFKALEP
                     RSPQQLTEGALRGMVGVLDPYSSYISEEDLPKFEEELDRQFAGIGVEIFLDPQSQRLC
                     VASPLPNTPAQKAGIKPGDWIVAIDGQATEGMTLEEAGKKMRGEPGTQVVLSVQTPGE
                     EKPHEVKLTREIIRAETILGDTRNPDGTWNYVLQSHPEIGYVRISTFGEETTAQLKKI
                     ILDLKKKNIRGLILDLRDNPGGRLEEAIAMCDLFIESGVIVTTRYRNGQIKRQYTASG
                     KPVCPDLPMSVLINHYSASASEIVAACLQDHGRATIVGERSFGKGTVQELIQLEAGLG
                     MLKLTTAAYWRPSGKQIHRSANAGEDQEWGVRPDPGKEVKLDRAEQDMLARYRALRDI
                     YLPPGSPKPAGFDSIPADFRDRQLEVAVESILKGQKTSAKSAE"
     gene            11091..12179
                     /gene="hydE"
                     /locus_tag="PKI05_00045"
     CDS             11091..12179
                     /gene="hydE"
                     /locus_tag="PKI05_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOA50611.1"
                     /translation="MFGTTVKEPALTREELLTWLREEDPARLSQLWQWADRVRQEFVG
                     GEVHLRGLIEISNHCVRLCAYCGLRAPNTELVRYRMSGDEILACARQAAALGYGTVVL
                     QAGEDPGLTRDFVREIVRRIKETTGLAITLSLGERDEEELWAWRQAGADRYLLRFETS
                     QAELYQRYHPPKPGSTVDRLYLLKRLREMGYEVGSGVMIGLPGQTYAGLANDLWLFRE
                     LDLDMIGVGPFIVHPRTPLADPAFARILPDGEQVPASELMTYKVIALSRILCPRANIP
                     STTALATLNRKNGRELGLVRGANVVMPNLTPPQYRIRYEIYPNKACLRETPDECHACL
                     QMRVESLGRVVGRGPGTSPNYRYRMAKD"
     gene            12273..13697
                     /gene="hydG"
                     /locus_tag="PKI05_00050"
     CDS             12273..13697
                     /gene="hydG"
                     /locus_tag="PKI05_00050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOA50612.1"
                     /translation="MSHVTRSHRLSQSAVDFIDEPYLFSLLEQPAQAGEVRDIIAKSL
                     SKEPLTVPETAKLLTIQQPDLWEEVFDAARQLKRDVYGNRIVLFAPLYIGSECTNDCV
                     YCAFRRSNPNVVRRTLSPDDIESQVCHLLDRGHKRLILVFGEHPRYSPEFIADCVRRV
                     YAVKRPRANIRRVNINAAPLDHEGFATVKAAGIGTYQIFQETYHHETYAKVHPRNTRK
                     GDYLWRLDALSRAMEAGCDDVGIGALFGLFDWRFEVLGLVSHAWHLQRHYNVGPHTIS
                     FPRLRRAAGVTLDETYRVSDEDFKRLIAVLRLSVPYTGLILTAREPAELRRAVMEFGV
                     SQIDAGSRIEVGGYTEVGDAQVLEREQFELGDLRPLDEVIRQLMTDGYIPSFCTACYR
                     LGRTGEHFMEYAIPGFIQRFCTPNALSTLMEYLVDYASRETYETGVRLIENELAKLPD
                     GPRKTALIERLRRIRETEERDLYF"
BASE COUNT         2888 a         4012 c         3991 g         2930 t
ORIGIN      
        1 cggtcagccc ctcgatagag ggaatcgcgg aggacctcag ccgccttggg gggacccatg
       61 ctaatgaccg tcactgttcc cccccagcga tcccgaattt cgagggccac ttccagagca
      121 ttcaaatctt ccggattaaa aatagcagga agagcggccc gattgacagt cccgtcttcc
      181 ttcatcgcct ccgcggtgac gttggcggta tcgggaacct gtttcacgca aacaaccaca
      241 tcgtagggca cgttctcggt ctccgtgatc agtttatgaa ctggtggatg aaacgcatca
      301 tcagccgatc agcccggcca cggccggtac gtcaatatat ttgttgtgtg tgcatctaca
      361 tcgtcgattt agggatatcg cccaacccga tctagcgtta accccactcg agatgccgaa
      421 aaacggcact gggtgacaat ctcccctggc ccgatcgtcc ggatggggca acgtggcttt
      481 ccggttgtcc gctacagcga cctggccggc gtccacggac tgtcggcttc gagcgaaggg
      541 gaaggcctcc ctatgggttg gtaaaatgca tattgtagcg aaatttctcc ctgggcggaa
      601 caccgctttt ttggcaaaac accccctcta cccccacagg agattccggg aggtacccaa
      661 aaccatcgcg caaaaagccc ggctgtcgcg gtttttcctc cggccgattt gacaagtcgg
      721 agtgtgggtt ctgcacctaa ccgcagagac caggaggcac ttcagcgtca agggaccgag
      781 gccaaatcgg tggcgagcgg ccttacgacg aagccgccag atagttcgcc agaatgcgga
      841 gattttgaat gaacgtccgg gttgtgatga ttcgtgtcac gcgggcatcg gcgggtgcag
      901 tcttcagacg cccagattcg acccaccgac ctcgttcgtc cagttgctca atcacccggc
      961 gggcttgagc cgcaagggat gccgacagtc tcggggccgt gggcaaccgg cctaatcggg
     1021 gtcgcggtcc cgatcgcaac gcctcttcat acgcacgttg gatcgcatct agttcgttgc
     1081 ccaccttgaa tgcataatgc gtcggcatgt cggcgtcact cgttgttaac cgataatcgc
     1141 gtgtgaagta gatgggctga ttggtcttta gctcatagaa gcgggcaagc ctcccgtcgg
     1201 gcaacagcga tcgtcgaaga tattctattg cccgcggaat cggctccaaa aaacgtttct
     1261 ccccagtctc gtggaaaagt gtgatcaacg cccgcatcac cccctgagac tctaggccag
     1321 tgatggccgg tggttcaaac ttgcgtgccc agcagggttg catgtgggcg tcatactgct
     1381 gggcccaggc cggctgaggg tccggcattt gggccttcaa caggaattcc cctgcgcgac
     1441 gtgccgaaac gaggtacttc tcatcgccat agatccgcca agcaagaagg accgtggcca
     1501 gggcgtcggc ctgggtattg tcgttgatcg tgtagcaatc tccgtagggg cgattaggcc
     1561 attggcgggg ccagtcatcg ggataggacg ccggaacgtc aggatactcc tttgggtcgg
     1621 gtggcgccac aaagcgttgc ggccagcctc cattgggacg ttggacggcc aacaaggcgt
     1681 ccaggccgta cgttacggcc tcatgcacca cctcatcctt catgccggtt tcccgatcgt
     1741 acatcatgag aaaccgcaag gcggattggg tgttgtcatc atcaagtgtc gagctattgg
     1801 cggccttctc gccaccggtg ggtggttttc ggtagcgata gcgagaacgg gcctgcgggg
     1861 caaattcgat ccggtaatcc catcccccgg agacaagctg ccccttcacc agggcatggg
     1921 cggtcctcgt tgccgcctga agacaaagcg gggccttcgt cagatggtag gcctgcaaaa
     1981 gggccatgcc cacactgggt gtccccggcg gttggaccca ggccgtctgg ggcgtcgcct
     2041 tcccttcccc ttcgcggaga gtcaaatcct cggcataagc ccaaacgtat cccccttcga
     2101 tagagacttc ctgatcgaaa aacgtcacgg cccgacgtaa ggcctcttcc gcggagcgcc
     2161 gcaaggccgc gtcaccggct tcgtttgccg ccgacaaccg cggaacttgg tgaagtgatg
     2221 cccaccctcc tgcgaggagc gcaaagcgac aaaaatcacg gcggagcatg gggatttcct
     2281 ttctcggctg aaggtctcaa tttgttagcg tcaaaacaag gtgctcatcc ttcccgaaac
     2341 caccgggccg agcgaaggtt ctgcttcttt tcttctactt ggaagacgcg gcgggcattt
     2401 ccgtgcatga tcggatcaat caactcaagc gcatcatcca aactgagcca gccttcttcg
     2461 accaactcag aaagcgccag ggcgatcccc tgccgggcaa tcaaggcatg gccgaggaca
     2521 ggctccaccg ggcgatagtc tccgccaaat gtgaaaatct tgttagcggg cgccgtgacc
     2581 aagaactttt tgagaaagtc cttggccgcc acagggttga tgatccagct ccaacacata
     2641 tcaacatagg cattggtgta ttgctttgcc acggcgatta actcttcata gtagggatag
     2701 cagatgtgca taaagacgaa cgtcgtctcc ggcgacatac gacacaaatc gcaggccgag
     2761 ccggcgttca atcgcaatcg cgaaaggggc atccggttat cgccggcgta atatccggtg
     2821 tgcaacttga cgggcaaatg gtgctccgtg gccttgccca cggcatacca gaacaaatgg
     2881 tcttccaggg ccttctgttc ctcggcgctg agggattctc cgttaagacg tttggcgaac
     2941 aagggctctg cccgttcttg cggaactttc tcgtagtcga tattccggct gtaggcattt
     3001 tgggacttga cggccacggc atatttcgcg tagcggtcaa accaccaatc gatgacgcgg
     3061 taccaatcgg tcaaggtctt gacctctatt ccggtagggc ggccaaaggc ccggatatct
     3121 ggtcctgcga acatgccaac aatgctgata tcctgcaaca agagaagggg ctgtttcgac
     3181 tcgtgatacg ggccgaggtt gacttggcaa gagtcgatct tggcaagatc acagaggatg
     3241 cggcgataaa agccaggccg tcgcgtggcc tgatactgct ctcgtacctt gtccaccgtc
     3301 ttttccgaga ggtcatcgat cccgtagatt tcccgcatgg cgattcgcac ggcttgcccg
     3361 tagccggtgt ttttcacggc tggccaaaat ggggccaaaa tctgccattt ttcctgcggt
     3421 gagcctggtt tttcaaagag ggccgcgtat tgttcgcgac tcattccggc tgcgcggaag
     3481 tccgaatcga gatagtgccg caaannnnnn nnnncgtctt ggggtgttcc tccgggaccc
     3541 cgcaagcagt catcttcgtc gggaaggtgt tcgtgactgt cgatgagcgg cgttttccaa
     3601 acgcgatcga agatgtggcg gcggacatcg cttgtcagcg gaatggaggg ggcctccacc
     3661 gatggcaaag cacaggcgga acccgcagcc atcatcccac tcatcccaac agttccaagc
     3721 agcttgagcg tctcgcggcg actgaaatga ctcatggtat gcctcccgtt tgtgtgaggt
     3781 gtgcggtcac cggcgtgcca gagtggggac gcctttcccg gccaattcca gcgatgattg
     3841 taacgccccg cagtcgtcaa gacgagccgt ttggaagaaa acggatgaga gagccgcatc
     3901 ccaccccaat cccaccggga gggtcattgt caaagatgag gcaatttacc gggagaatcg
     3961 ccgtccgcgc cgaccgaatc acctattccc ccgtaggggc aaccccctgt ggttgccctg
     4021 tttggtcaca gcgccggtgg tttacgtttg gccaggccca gattgtttgg atttcacgcg
     4081 gacccgggcg ctggtcacgt tctggggcag gcacaggggc ctgcccctac ggggtttcaa
     4141 tgggctccta ttcgcccgga actacaggtt attttgaaag gaggatagcc atgcgacggg
     4201 tttcaatggg ctcctattcg cccggaacta cagggtatgt accgtaagtc ttttctgatg
     4261 gtaatcttac ggcaaaaata cctgcaaaaa actcgcgtca aacggccttt aagcggccca
     4321 aaatcgtcag ttttttgcga gtttttcacg ctcttcgccc cacataatac cgccctgccc
     4381 atggcgttgc aagcccccac cacaaatctt cacgaaatca atatacgcgc ccagcccggt
     4441 agatgcaaca cccacacgcc cgggatgtca cgcatatcgc atcggacttg gatttcgcag
     4501 ggtcccgggc cctgtttgcg ttttgcggca ggcacagggg cctgccccta cggcgtttca
     4561 atgggctcgg caatcgtagg ggcaaccccc tgtggttgcc cggtttgtcc gtagcgccgg
     4621 tgcacacagg acggcgagga cgccgtccct cccgttcaca gaactatcca caaagctcaa
     4681 gaagaccgct tctcccaggg ggaagtgatt tgggcatgga gaccgtacct ctggcaccct
     4741 aaaggtctat cctaaaaagt ccccctgtcc ctccgggaga ggggggcttt ttgggcttag
     4801 gagcatccct tatcccaaat cccccaacac gtgcccaatt tcggcagaag catcaatggc
     4861 cgcaatgatg tgctccgccc aagttcgcgg atcattcgcc tcggagaaca ccacaggtgt
     4921 ggcctgatcc cgaagccaag aaagatttgc caactcgaag gagggactca caagcggccg
     4981 atctgatgcg tttgcctcat gcggccacaa caagtccttc gcccgaggac cgaggatcat
     5041 cacgcgaggc gggggagtct ctcgcccatc acgaacctgc ttgcgatgct ccaaccaagc
     5101 gaatgacaca atctccgcca gcgattgacc catcgccaag cggtgttgaa cctcctccgc
     5161 gttaaatatg caactttccg gtgggtgggt aaggtgcaca ccggccgaga ctgatccccc
     5221 cgagtcctcg agatgtgggc atcctgcctg aaacaccttg ttcgcggcca cgcacgcctg
     5281 ccatagggcc gcccagtctg cctcgcccgc ctgaatctcg ccgctgtggg tcgtcaagct
     5341 tgatgcagca tgggtgggca gaaaggtctc ccaaattaac acgcaatatg gtggccgggt
     5401 ggaaagaatc tcaaccattt gccgcacggt caaaccgcac caagggtgtc gccagtcggg
     5461 aagtagctct tccaaggagg ccagaaaaaa tagccgtgac cccaaccgcg gatggggaat
     5521 caggagccgc tggtcgtaga acgggccggc ctcatcaagg aggatgtcca agtcaagtgt
     5581 ccgcgatgcc cagtgacgat tgcgttcgcg accgaacctt tgttcgatcg ccatgattcc
     5641 atccaaaagt tcccgcggag gaagggccgt ctccagaagc gcgactgcat taaaaaaggg
     5701 cgagccttgg tctgacactc taatgggctg ggtaagtctg atgcggctgg tagcgcgaag
     5761 ctgggtggcc ggcaactcgc tgagggcctt gcaggcctcg cgaaggatcg cccacgaatc
     5821 accgaggttc gatcccatcc caatggcaac caagcgtggt gcggagcttg caaggtctgt
     5881 cattaattcc catcactcca atttggacgt ggcaagcccg attgcctgcg aggcccttag
     5941 cgggagatga agcccaggtc caggccaaag acccagagca tgatccccag gatgattgcc
     6001 aatcccagat aggataggcc gacatagacg gtttcactcg gcggcttccc gcgaattccc
     6061 tcgtaaataa gaaacgccat gtgccctccg tcgaggaccg gaatgggcag gaaattgata
     6121 acggccaaat tggcactcaa cagcgtgagg aaaagtaaaa accgaccgag gccctgcgag
     6181 gccacccgat aggccgcctg gaccaacccg atagggccga ccaaagcccg gggcgagaca
     6241 tcacctctcc ccaggctgtt caaaacgcga tagatcaggg tcactgatgc ccaggtctct
     6301 gcggccccca tggtgatggc ttctcccaag cctgccgccc tgaggtcgaa ggtcatcgct
     6361 tcaaaaagca agtcgcgctc ggccaggaac caatcatccg caacgcgcgg aacgagatgg
     6421 acctttactt cctgatactc ctgggcaggg tccggctggg gacgtcgtac cacgagatcc
     6481 acctcagtcc ccgtcggcat cctctgcaat tcccggaaga aaaagggcca ggtgcctttt
     6541 tcttcacctc gcggttgatc gaagcggacg gtaaacttcg tcatggagaa gtgtctggca
     6601 agctctccga tctttgattc ggggtcgagg ttgggcggga tggcggtcac gctgattagc
     6661 tcgtcccccg gctgtaatcc ggcagccgcc gccggcgaat gcggttctac cgcctcaatc
     6721 tggggcagaa tctctactgc aatgccaagt tgagggatct cgaagggact atcgcccgta
     6781 ttgagatcgg cgaagaactc ggcggttttg gccactacgt caagcttcag cggagtgcct
     6841 tcgttggagc tgtttttatc ggcggcggtg gccgaacgca tggccaccaa ggcgacgcct
     6901 gccttctccg agccgttcga tctcttcacg gtgagttgaa ggggaattcc ctcgggccga
     6961 tcgcggaggg ccttttggag ggcgtaaggt agccgcatga agttgccggt ccagttcacg
     7021 ttgtcgggac aggtaagact catcaattcg tcgcccgcca ggacccctgc aacctcagcc
     7081 ggggagcccg cccgcaccgc gacaattcgt cccaccttga aaatcaaccc caagtcacga
     7141 accggcgttg gctggaggac aaccttctgg ggctcgagag gcgttccgct cgcagccttt
     7201 ttctcgagcg ttagctccac gggctgaccc caggacttgg cccaggatcg agcaatgtcg
     7261 gcgaagccag tgacgggctc gccatggatg gccttgaccg tggcgcctgg cggtaagctc
     7321 gtgataactt cggcggcagc gctccccgga taacacggcg gctcggccag gatcggtaac
     7381 agtggatttg tcacgccaat ggtcggcacc acgcggattc ggtcgggcat aagttctagg
     7441 tccaatacct tgagaccgtt tggggtgggt cgttcgatcc gcatggtgac gacttgcccc
     7501 cgccgcgcca acgagatgga ctgctggaga tcttggaagc ggtgggtctt ttgaccgttg
     7561 atctccaaga tcttgtcacc cggcagaatc cccgcccgcc atgccccctc tcccggcgca
     7621 agttggccca caccacaggg aatctcttga accccgataa aataggccag caccgccgcg
     7681 atgaaggcaa aaagaaggtt catggccacg cctgcggaaa tgatggccat ccgttggggg
     7741 acactcttgg ccaggtagct ccttgggttg taaagggcct cttccatgtt ggccaacgat
     7801 tcgccggtgg aggacttggc tccttcctga ccggcggcct tggcgcgttc aaattcctcg
     7861 cggagtcggg cggggttatc ttcctgcccc aacatcttga cgtaaccgcc aagtgggatg
     7921 atgccaattc cgtattccgt ttcgccccag cgaaaacgaa aaagccgcag gccgaagaaa
     7981 tcgaatccca gataaaactt ctcgcatttg acgccacaga gcttggccac cacaaaatga
     8041 cctaactcgt ggacaaagat gatcagcccg aggccgacga tcaccatcag cacgtacagc
     8101 cacgcggcac cacccaggaa agcaaaaaag agcgagttta tatccaacgt tcaacctcct
     8161 tacgggccca ggcgtctaga ccgaataccg cgtcaagatc aggggatggg tcaaaggggt
     8221 gggcttccag taccttggca cacaccggga caatctgatc aaatcgcagg cgtccctgaa
     8281 gaaaacattc tacagcggct tcattggcgg cattcaggac ggctccggcg gtccccccga
     8341 tccgagcggc ctccagtccc aagcgaatgg caggaaatcg ctccaaatcc ggtggctcaa
     8401 atcgcaaatc catggccgtc gcccagtcga ttttgcgggc gatgccgggc cgtcgttcag
     8461 gaaagtataa cgcatactgg atgggaagtc tcatatcggg gggactcagt tgggcaatca
     8521 ccgagccatc cacgaattcg accatcgaat ggacgatcga ttggggatgg ataacgaccg
     8581 tgatttggtc ggcctccacc ccaaatagcc agcgggcttc aatgatctcc agggccttat
     8641 tcatcagtgt ggcggaatct accgtgatct tttttcccat tttccaagtg ggatgggcca
     8701 gcgcctcttc caccgtgacg gaggccagct cggtgagctt tcgactgcgg aatggcccgc
     8761 cgctggccgt caagataatc cgccgtacct ctcgttcctg gccgccgtgg agggcctgaa
     8821 agatcgccga atgttcactg tccacgggaa ggatttgggc ctttcgcttt cgggccaatt
     8881 gcgtgatgag ggacccggcc accaccagac tttccttgtt ggccagggcc acagttttcc
     8941 cggcctccaa ggcggcccag gtggccctca gccccgcgat tcccacaaaa gccgaatgga
     9001 ccatatccac atcgtccgcg gaaacggcct ccaggacggc ctcgtacccc acccgcaatt
     9061 ccgtctccag gggcaagtcg cgccaagagt gcgctgccgc agccttggga tcagtgacaa
     9121 tcaccaaacg tggcctctgc tcttgggcct gttgcagcaa cagttgtgtg tttgatcgcg
     9181 caaccaaaat gatggcgcgc aaagccccct gggaggcgga aatgacttcc agtgcgctgc
     9241 ggccgatgct ccccgtggag ccgaggacgg caatggtctt tgtgcgattt ccccaacttt
     9301 ccgataatgt tgactcttgg gacttcacct gtgcagccac gtcacctctt tgacacgttc
     9361 gctgttattc cacaaaagtc tttttatccg caacttttcc ctgtcaggca accccgtttt
     9421 attctgccga ttttgctgaa gttttttgcc ccttgagaat tgattccacg gcgacttcca
     9481 gttggcgatc gcgaaaatct gccgggatgc tatcgaaacc ggcgggttta gggctccccg
     9541 gcgggaggta aatatcgcgc aatgcccgat atcgcgcaag catatcttgc tccgcccgat
     9601 ctagtttcac ctccttgccg gggtccggcc tgacgcccca ttcctggtct tcgcctgcat
     9661 tagcggagcg gtgaatctgc ttgccgctgg gccgccaata cgccgcggtg gtgagtttca
     9721 gcataccgag gcctgcctca agttggatga gctcctgcac cgtccctttg ccgaacgacc
     9781 gctcgccaac aatagtcgct cgcccatgat cctgcaaaca ggcggcgacg atttcgctgg
     9841 cgctggcgct gtaatggtta atgaggacgc tcatcgggag gtcgggacaa acgggtttcc
     9901 cactggcggt gtattgtcgc ttgatttgac cattccgata gcgcgtcgtc acaatgacgc
     9961 cggactcgat aaatagatca cacatggcaa tggcctcttc cagccttccc ccgggattgt
    10021 cccttaggtc caggatcaat ccgcggatgt ttttcttttt caaatcgagg atgatttttt
    10081 tcagttgggc ggtcgtttct tctccgaagg tcgagatacg aacgtagccg atctcgggat
    10141 ggctttggag gacgtaattc catgtgccgt cgggatttcg ggtatcgcca aggattgttt
    10201 ccgcgcggat gatttcgcgg gtcagtttca cctcgtgggg tttttcttcg cccggggttt
    10261 gtacggaaag cacgacctgg gtccccggtt caccccgcat cttcttcccg gcctcctcaa
    10321 gggtcatccc ctcggtggct tgtccatcga tggccacaat ccaatcgccg ggtttgatcc
    10381 cggctttttg tgccggggta ttcggcaggg gactggccac gcacaaccgc tggctctgtg
    10441 ggtcgaggaa gatttccacc ccgataccgg caaactggcg gtcgagttcc tcttcgaatt
    10501 tgggtaggtc ttcctcgctg atatagcttg aatacgggtc gaggacacca accatgccgc
    10561 ggagggcacc ttctgtaagt tgctgcggcg atcgtggttc gagggcctta aattggattt
    10621 gttccaacgc gtatgtcaaa acccgggtgt atcgcgaact ccggtaggca cacagaagac
    10681 agataataat ggccaaaacg aggatttgaa gatttcgacg cggcatatat caatccctct
    10741 tggggtagtt ccctaagacg caatcaggta accacgggca gctcgcaggt cagaacgatc
    10801 cttccggggc cggtgtgtgg caatcggtct tggattgggc gcagaccgta cgaacctccc
    10861 ttttttgagg ttacccgcca ttgagaaaaa agggaagaat gatcaccgcg cgacgtacga
    10921 ttgtgccctt ggcggaaagg tgaggagagg gcaaggaagc acttctctcg gctgggagtc
    10981 gtataattca aaaggcgctc tagatttgcc aagcggcgcg gaactcgaag ccattttgca
    11041 gaaaagacta acttgtccat gcggcgtaaa tgaaggggga aaactcccct atgtttggaa
    11101 ccacggtaaa agaacctgcc ctaacacgcg aagaactcct cacgtggtta cgggaggagg
    11161 atccagccag gctgtcccag ctttggcagt gggccgacag ggttcgtcag gagttcgtcg
    11221 gcggggaggt tcacctgcgg ggattgattg agatttcgaa tcattgcgtt cggttatgcg
    11281 cgtactgtgg cctgcgggca cccaatacgg agctggtgcg gtaccggatg tcgggggacg
    11341 agattttggc ctgtgcccga caggcagccg cgctgggtta tggcaccgtg gtcctgcaag
    11401 ccggggaaga cccggggctg acgcgcgact tcgtcaggga gattgtgcga cgaatcaaag
    11461 agaccacggg ccttgccatc acccttagtt tgggcgaacg agacgaagaa gagctttggg
    11521 cgtggcggca ggcgggggcc gatcggtacc tcctccgttt tgagacatcg caggcggagt
    11581 tgtatcagcg ctatcaccca cccaaaccgg gctccaccgt tgatcggctt tacctgctca
    11641 agcgcttgcg tgagatgggc tatgaggtgg gcagcggcgt catgatcggc ctaccgggac
    11701 agacctatgc cggattggcg aacgatcttt ggctatttcg cgaattggat ctagacatga
    11761 tcggcgttgg gccatttatt gtccacccgc ggacacccct agccgatccg gcctttgcca
    11821 ggatccttcc cgacggggag caggtgcctg ccagcgaatt gatgacgtac aaggtgatcg
    11881 cgttgtcgcg gattctctgt ccgcgggcga acatccccag cacaaccgcc ttggccacgc
    11941 tcaatcgcaa gaacggacgg gagctgggac ttgtccgcgg ggcgaatgtt gtcatgccca
    12001 atttgacgcc accccaatac aggattcgtt acgagattta ccccaataag gcgtgtcttc
    12061 gggagacgcc ggacgagtgt cacgcctgtt tacagatgcg tgtggaatcg ttaggacgcg
    12121 tcgtcggacg tggtccgggg acatcgccca attatcggta tcggatggca aaggattgac
    12181 tatgcgttct gccagaggaa attggtcggg ccgtcgttga gcctggcctc ccataggatg
    12241 gagttgcccc acaatcattg cgaggtatcg acatgtcgca cgtgacacga tcccaccgcc
    12301 tgagtcaaag tgcggtggat ttcattgatg agccatattt gttcagcctc ctagagcaac
    12361 ccgctcaagc gggcgaagtc cgtgacatca ttgccaaaag tttgagcaag gaaccgctca
    12421 cggtaccgga gacggccaag ctgctcacca ttcaacagcc ggacttgtgg gaggaagtct
    12481 tcgacgccgc tcgccagtta aagcgggatg tttacggtaa tcggatcgtc cttttcgccc
    12541 ccctttacat tggcagcgaa tgcaccaatg actgcgtata ttgcgcgttt cggcggagca
    12601 acccgaatgt ggtgcggagg acgctttcgc ccgacgatat cgaaagccag gtctgccatc
    12661 tcttggatcg tggccataag aggttgatcc ttgtctttgg tgagcatccc cgttattcgc
    12721 cagagttcat cgcggattgc gttcggcggg tctatgcggt caaacggccc cgggcgaata
    12781 tccgccgcgt gaacatcaac gcggcgcctc tcgaccacga gggatttgcc accgtcaagg
    12841 ccgcggggat cgggacgtac caaatcttcc aggaaacata tcatcacgag acctatgcca
    12901 aggttcatcc ccgaaatact cgcaaagggg attatctgtg gcggctcgat gcccttagcc
    12961 gggccatgga ggccggttgt gatgacgtgg gtattggtgc cctgtttggg ctattcgatt
    13021 ggcggtttga ggtcctggga ctggtcagtc acgcctggca ccttcagcgg cactacaatg
    13081 tgggaccgca cacgatcagt tttccccgat tgcgacgagc ggcaggggtc actttggacg
    13141 agacctatcg cgtctcggac gaggatttca aacggctcat tgccgtgtta cggctttcgg
    13201 tgccctacac cggactgatt ctcacggccc gcgaaccggc cgaactccgc cgggccgtca
    13261 tggagttcgg cgtttcgcag atcgacgccg gaagccgcat cgaagtcgga ggttacacgg
    13321 aagtgggaga cgcccaggtc ttggaacgtg agcaattcga gttgggcgat cttcggcccc
    13381 tggatgaagt cattcggcaa ttgatgaccg atggctatat tcccagtttt tgcacggctt
    13441 gttatcgtct gggacggacc ggcgaacact tcatggagta cgccatcccg ggatttatcc
    13501 agcggttttg cacgcccaat gccctgagca ccctcatgga gtatttggtc gattacgcaa
    13561 gtcgggaaac ctacgagact ggggttcgac tgatcgaaaa cgagttggcc aaactccccg
    13621 acggccctcg caagacggcc ctcatcgagc ggcttcgtcg tatccgcgaa acagaggaac
    13681 gagacctcta tttctgagaa cggtctatcg ccccgtttgg agcctatccc ataatcttcc
    13741 ctcacgcgtt cggcaggaaa ggggtcgggg gtgacggtgg ctcgaatcgc tgctggtttt
    13801 ctcaatcgat tccgaggttc gcttgccctc a
//
LOCUS       DAOPTR010000002        12198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_4386_length_12198_cov_4.035576, whole genome shotgun sequence.
ACCESSION   DAOPTR010000002 DAOPTR010000000
VERSION     DAOPTR010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 12198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12198
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4386_length_12198_cov_4.035576"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1978)
                     /locus_tag="PKI05_00055"
     CDS             complement(<1..1978)
                     /locus_tag="PKI05_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50613.1"
                     /translation="MKSSTRLKENTFGGCGGAAQRILAVAASASRDGLGKTSGKRTPS
                     AAGHSVVGSLDGKARFPNIVAEFTRWPPLGNRLWTYCVVAVGMILSLWFSVAACGEEN
                     GLRISWGGGSARRWAGRISVSQGEILRWQPIGSEADELASLVFRDGGIDIVPTTPRIF
                     QGVDLALDRQGQTLLEIELAAEGTPSQQWSVPIQEIERQPRQMALSGGGQLFIERLPG
                     DELAVSTDRPHLVFSPGETWKFYVTPRGLPFSPGTHVDIRIHIVRSRITDREPRRPLL
                     DSLADLRRTGNTDAPVVRYEITVGKESPIPVEITLPEEEGCYDVVITATPIPSFRLAE
                     SVTRPFGGNASNLERHVQVVVLNTTRRTGEAFSGELTLLGEAVDASNQNFWERLSRVA
                     PYLPRLGFLGRGWRHIGGEGQEAVCELAAASPGEDPAWALYSLPVQEPGRPHLLEIEW
                     PADQRQTMLFSIVEPNAVGAIAPPMVGYGVYSTGEFRRWEQPLGWQVHRVVFWPRTRQ
                     PLLLVANPSGEPALYKKIRVYAGWNRLPEIFPASAFPSERLVAAYFHRPILSTLFGAT
                     DVPGPFQQQGLDDWVTFYQAATRLAEYVTFAGYNAVVLSVAADGSTLYPSERWKPTPR
                     YDSGSFSLTGADPIRKDDLELILRNAHRERYSAIP"
     gene            complement(2153..3979)
                     /locus_tag="PKI05_00060"
     CDS             complement(2153..3979)
                     /locus_tag="PKI05_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOA50614.1"
                     /translation="MTRRVVILGTTLIFAAGCSLWFNSKKASSLLDPVETKTPSLVGD
                     LAVPYGLHPIVVEAIGLVTGLNGTGSDPLPSPERQFLLTEMQRRGVQNPNRVLASKNT
                     ALVLVRGYLRPGIQKGENFDVEVRVPSRSETTSLRGGTLLEVPLREMMVLRGAVREGH
                     ILAKAAGPVLVDPSADPEKDAIYATRGRILGGGTADRSRTLGLVLKPDHQSVMNSARI
                     ESAINRRFYVIRQGVQEGVARAKTNEYVEVRLHPRYKNNIARYMAVVRSIPLRDTEPE
                     RLQRLRDLEEELLNPATSEQAALQLEAIGQPAVETLKKGLTHADPAVRFYSAEALAYL
                     DCAEAAGVLGEIARNEPAFRVYALAALSTLNDLETVEILHDLLTSPSAETRYGAFRAL
                     VELNPNDSVIAGEKLNDQFWYHVLRVSGPPMVHVTRNRRAEVVLFGPDITLKSPFALE
                     AGHRIMVTSQQPGKVTVAKFAVNEADQKRFVSNNLDEIIRAVAELGGTYPDVVQMLQQ
                     AKAAGVLEARFEVEAIPETGRKYQGPSEDESPSPDVAPLVKKYEELPGGGDVTTDHAL
                     PGPEFDASAIDTEPTPAEEQNVSETSKKGRSWWPFGGTKTSS"
     assembly_gap    4324..4423
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4531..6105
                     /locus_tag="PKI05_00065"
     CDS             4531..6105
                     /locus_tag="PKI05_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50615.1"
                     /translation="MVISISKLPKGLRYCLCHKLFWNHTLRLASSEKLIILGRLEPFR
                     AFWIIGAVFVFSIITGGTACQRQASPDHDSPAVKESVVSQEQAHRESPAEPVNPPQYR
                     AQKGPIEFELVQRLAYGTPEEEGTVPPEFAEIIRGLLAARASLEFDTNGRLIGVDLAG
                     ERRSGSDAEISLAVRLPHLKRFRVAGFGVTNEGIKQLATCQNLEELGLENTQIDDAGL
                     EYLKALPRLWSLNLRRSVKLTDRAIDTLVGFPRLTHLYLLENQFSAEALMKIAQMDRL
                     RLLDLRGCGAVNSEVLRRLSAMEHLAALRLRGYNIDDVCLQAVGGFSHLTSFTLEESP
                     AGNAGLASLARLPLETVTLFRCTAVNDEGLRIVSQWKKLRSLTLRDMAIRGDFFKELG
                     EVPQLRSLSATQTMVSDDALHHLVACQQLEELALRQTLVTDKGLAVISRLPNLRRLDV
                     AQTMVSDAGLVHLANLKSLEWLDFSGNSGVTDASVKILLKMPQLKEVRLDGTSMTQEA
                     IAQLGPRARTALPVEE"
     gene            complement(6151..7119)
                     /locus_tag="PKI05_00070"
     CDS             complement(6151..7119)
                     /locus_tag="PKI05_00070"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HOA50616.1"
                     /translation="MSVDSSRQQIHVRFRGRVFGPYSADELRRMVQKGKTAATWEISL
                     DGQNWRPIQELETLLQQSHSVSTWKGPSPSSHGPLGTINDADDDVFVLRGTNAEDAQD
                     IVLPNLQQPEPVTAEVVSSESLWYYTVNDEPEGPIPESHLAAMAATGLIPPDTLVWTV
                     NMSEWRPLRETRLARHLQSARVEQPATVSSLPPAKDFDEEPREAHVRQTGLTFAEELE
                     MVLDEDAQHLNLWFRVLLGAAILKALWIVVSFVSGGALSWVGLLCDMGITGSVIGISI
                     LMLRTIARLRLGTRFIAHPKRLDELYSKEKPKRRESPWSNPVTEDE"
     gene            complement(7116..10031)
                     /locus_tag="PKI05_00075"
     CDS             complement(7116..10031)
                     /locus_tag="PKI05_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50617.1"
                     /translation="MTGDQTIVDRIEQALASLDAVSNEELHQLAEEYAEACEQVNRRL
                     QEIHHLIRSGARSEAIRRAEIPPKLLDVVEILDFPDRDAWVDICTLERLPTPPRLLVQ
                     YLSELHQAYEAEQGLSGLLRQHRALAMAQAPLHKRLAILRQLARAEPENPVWQKDIRI
                     LENYWLDKLQREIQERLKAEDLQTLEEILEQLESNEWLEKPSVSLVEQCRSAVASLRG
                     KIFRQEMENIARLVNQALANGDMVRMEEYLRLWDHQAATNPQWVDIQLREQVAEAMSR
                     RHQLEREREEARRRRELLTEFEALLGKSHDLHTLEHHYAELAIRGVAIPEPLEERYRR
                     VTETLRQRAMFRRVVTVAGVAAVVIATLLIVWQVQARMVENRRLSDAVSVVEDLLEKD
                     KLDEAAKYLADFKAAYPTLAQHRHVISLEQKLADAQAAEAKRRETLASLLEKVRASLA
                     QVPDGVSLQKASELARTDAEKQEVMELESQAKVIWANLRQQAEERLRQEIAKLSREYD
                     ALRQRGSVSIDEELQAIARLMNRLTVLSDDNRRQGLSLEGEIRNLREQLSSRFLEVSQ
                     ASEEEKLIRTMCSAAARGPEAYQEAIKKLEPAKSVRIPIGDFQLVEQEFPCWKVIAEW
                     NRVATLWNDPTTSVSDAGAQELFRGAFRLVQVVPPEASAFVQEGQKRILALLDTIARR
                     ELLVADRLTVLVKEWRAPLVADAWQIRLNDGRRYYCLEQPGRTALVLRDLGSLQRNEI
                     TFPRESLVSTGRAPQSVLANQVRDHLADWSASNWETTADTVLGKIIGFCHTVEKDTVE
                     WPLIFHLLYETWSAAKEGDVAWAALWNGYQAKLQPLRDRYPPHYDWIKTAQAAENQYE
                     DIRSVLGSLPTAEDLLSQVKQVRATYRGRRWPTIDLVGLLFEDKTQVWYVRTVGQPAV
                     PDGAVLYVVEQGPEEGQVTLRRIGHWSNSRAVVEPQQSVRFYNGRHVVTLAGEEPGS"
     gene            complement(10028..12100)
                     /locus_tag="PKI05_00080"
     CDS             complement(10028..12100)
                     /locus_tag="PKI05_00080"
                     /inference="COORDINATES: protein motif:HMM:NF040416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50618.1"
                     /translation="MAWELSITSAPRGLHPGRAGFCPVAHTQGMPPQLIERLEKISGY
                     RHLEIGAGNESRNPVVYGHTVLRLGRETFHVLSRICDAGRDYSGRSNRFAYHLVLKPQ
                     ELVPAGPAELLQRPELMLDKWDGQLRVIPTEKEIPKITSEPRPCELWQKVLGDAGWAG
                     VLAQRFLQAPQETIYFVYPLQIDMLKLFQEVIALLPESLRWKVTFSTYYQGLGEVVTC
                     RWRAIPEIASEYAAIMKQQGPQAWDLRSKRGPAPATSEADAAREGRLIELPTPTKVSV
                     PVSEVPTPQPVTVPAPAPREPAFLELANSVDAPPPLWVASKRAVPERRKRRVPMATIK
                     SLATLGAGVIVGLGLGFVLWSGTIMRPLLPGFLANPAERELDQAAERGGEKPDFKQAA
                     RGDKQKPPERKPSPVPAQGNEAKNGEQPVAVGPKEPPQQGKPPGPPAGETAKSDQMTS
                     APPAEKPPEGKATGEANRAGGGERSVVSANGPPDQGNAVSEDEFVAAPISELRLKEIV
                     KIFLKKTPTGAPGVKTLQSWESQRLWSRECESTQDFFLLKVVDDNGQAQYYVLSMNSI
                     PVETLALGQEKTIEIPPLVGMTPEVRCVGGWKKGQTTSEFVFSVDGKDRARLKIERKP
                     AKITVKVDEVKQGDGPPLPKDLMAQIGFYASDGPKRVWCPIVDLQIRSGAKVESAREN
                     AQPPGGNR"
BASE COUNT         2444 a         3677 c         3421 g         2556 t
ORIGIN      
        1 cggggatggc cgaatatctc tcgcggtgtg cgttgcgaag gatcaactcc aggtcgtcct
       61 tgcggatagg atcggccccg gtgagagaaa acgaaccgct atcatagcgc ggcgtcggtt
      121 tccaacgctc actgggataa agcgttgagc catcggcagc caccgacaag acaaccgcgt
      181 tgtatccggc aaacgtcacg tattcagcta atcgggtcgc cgcttggtaa aaggtgaccc
      241 aatcgtccaa gccctgctgc tggaaaggac cagggacatc ggtggcacca aagagcgtgc
      301 taagaatggg gcgatggaag taggccgcca ccagccgttc gctggggaag gcagaggcgg
      361 gaaagatttc cggcaagcgg ttccatcctg cataaacgcg aatctttttg tacaaagccg
      421 gttcgccact cggattggcc accaaaagaa gtggctgacg tgttcgtggc caaaagacga
      481 cgcgatgaac ctgccagccg agcggttgtt cccaccggcg gaattccccg gtggaataga
      541 cgccatagcc gaccatgggc ggagcaatgg cgcccacggc gttcggctcc acaatggaaa
      601 agagcatcgt ctgccgctgg tcagccggcc attcgatttc caagaggtga ggtcggccgg
      661 gttcctgaac gggaagcgag tacagggccc aggcggggtc ttctcccggg ctggccgcgg
      721 caagctcgca gaccgcctct tgcccctctc ccccaatgtg ccgccaacca cgaccgagga
      781 accccagccg aggcaaataa ggcgccaccc gggatagacg ctcccagaaa ttctgattgc
      841 tggcatcgac ggcctccccc aagagggtca actctccgga aaaggcctct cccgtccgcc
      901 gcgttgtgtt gagcacaacc acttgcacat gccgttccag gttcgaggca ttcccgccaa
      961 aaggacgcgt gaccgactcg gccaaacgaa aactgggaat cggcgtggcc gtgatcacca
     1021 cgtcatagca gccttcttcc tcggggagcg taatttccac agggatgggc gattccttac
     1081 cgacggtgat ctcgtaacgg accaccggcg catctgtgtt gcccgttcgc cgtaggtctg
     1141 ccaaggagtc gagcaagggc cgacgcggct ctcgatcggt tatccggctg cggacgatat
     1201 ggatgcgaat atcgacatgt gttcccggcg agaagggcaa gccacgaggc gtgacgtaga
     1261 atttccatgt ttccccgggc gaaaaaacga ggtggggacg atccgtggac acggccaact
     1321 cgtctcccgg aagccgctcg atgaaaagct gtccgccacc cgacagtgcc atctggcgcg
     1381 gttggcgttc gatctcttga atgggcacag accactgttg cgatggcgta ccctcggccg
     1441 ccagctcgat ctccaacagc gtttggccct gccgatcaag tgcgaggtcc accccctgaa
     1501 agatccgcgg agtggttggg acaatgtcaa tccctccatc gcgaaacacg agggaggcga
     1561 gttcgtcggc ctctgacccg atgggttgcc agcggaggat ttccccttgg gaaacactga
     1621 tccgaccggc ccagcgcctg gccgagcccc cgccccagga aatccgcagc ccattctcct
     1681 ccccgcaagc agcgaccgag aaccacaggc tgaggatcat ccccacggcc acgacgcagt
     1741 aggtccaaag gcggttcccc aacggcggcc agcgggtaaa ctccgcgacg atgttcggaa
     1801 agcgtgcctt tccatctaat gagccgacga cactatgacc agcagccgac ggcgtgcgtt
     1861 ttcctgacgt tttacccaga ccatcgcggg aagccgatgc ggcgacagcc aaaatgcgct
     1921 gcgcagcccc accgcacccg ccgaaggtgt tttccttcaa gcgggttgat gacttcacac
     1981 agttcggaca tccctgtccg cacatcacgc cctcatcatg cggtcgtcca ccgccggttt
     2041 ttacggatgg tcgtaaaatg ccgcgactat ccgctcggac aatcgcggaa cttggggtgg
     2101 cattgcggtc ggtaagttgg caaggtagct ctggctcatc atcccgtcag gcttatgaag
     2161 atgtctttgt cccgccaaaa ggccaccaag aacgtccctt tttcgatgtc tcggagacat
     2221 tttgctcttc tgccggggta ggctcggtat caattgccga ggcatcaaac tccgggccag
     2281 gcaaggcgtg gtccgtcgtg acgtcgcccc cgcccgggag ctcctcatac ttcttgacca
     2341 acggtgctac atctggggag ggggattcat cttctgaggg tccctgatac tttcggccag
     2401 tttcgggaat tgcctcgacc tcgaagcggg cttcaaggac gcccgccgcc ttggcctgct
     2461 ggagcatttg gaccacgtcg gggtaggtgc cgcccagttc cgccactgcc cgaatgatct
     2521 cgtccagatt gttgctcacg aagcgctttt ggtccgcctc gttgacggca aatttggcca
     2581 cggtcacctt accaggctgc tgcgatgtca ccatgatgcg gtgacccgcc tccagggcaa
     2641 acggtgattt cagtgtgatg tcaggtccaa acaggacgac ctcagcccga cggttacgtg
     2701 tgacgtgaac cattggtggg ccactcacac ggagaacatg gtaccagaac tgatcattga
     2761 gcttttcgcc ggcaatgacc gaatcattcg gattcagttc aaccagggca cggaaggccc
     2821 cataacgggt ctcggcactt ggtgatgtga gaagatcgtg gagaatttcc acggtctcca
     2881 gatcgttcag cgtgctcagg gctgccaagg catatacgcg gaaggccggt tcgttgcggg
     2941 cgatttcccc aagaacccct gccgcctccg cgcagtcgag ataggccaag gcctctgccg
     3001 agtaaaaccg caccgccgga tcggcatggg ttaatccctt tttgagcgtc tctaccgccg
     3061 gttgcccaat cgcctccaat tgcagggccg cctgctcgct ggtcgcgggg ttgagaagct
     3121 cctcctccaa gtcccgaagc cgctgaagcc gctccggctc tgtgtcacgc aacgggatcg
     3181 accgaaccac ggccatatac cgagcaatgt tgttcttgta acgcgggtgg agacgtacct
     3241 ccacatactc attagtcttt gccctggcca caccctcctg aacaccctga cgaatcacgt
     3301 aaaaacgacg attgatggcg ctttcgatac gggcactgtt catcacagac tgatgatcgg
     3361 gcttgaggac aagtcccaga gttcgggaac gatcggcggt tcccccgccc aagattcgcc
     3421 cgcgagtcgc gtaaatggcg tctttttcgg gatcggccga cggatccacc aacaccggcc
     3481 cggcggcctt ggcgaggatg tgtccctccc gcacggcccc gcgaaggacc atcatttccc
     3541 gcaggggaac ctccagcagc gtcccccctc gcaggcttgt cgtttcactc cgcgacggaa
     3601 cacggacctc cacgtcgaag ttttcgcctt tttgaatacc gggacgaaga taaccccgca
     3661 ccaaaacaag ggccgtattc ttggaagcca ggacccgatt gggattctgt accccacgac
     3721 gctgcatctc cgttaaaagg aattgtcgct ccggcgaggg aaggggatcg ctgcccgtgc
     3781 cattaagacc cgttaccaag ccaatggcct cgaccacgat cggatgcagc ccatacggca
     3841 ccgccaaatc accaaccaga ctgggcgtct tagtctcgac cgggtcaagg agcgatgagg
     3901 cctttttgct gttaaaccaa agagagcaac cggccgcaaa aatcagcgtc gttcccagga
     3961 taacgactcg tcgtgtcata aaggcatcca tgccaaaaag gggatcactc tccacagcca
     4021 cttgggcaag cagattgccc aatacccgtt atgtcggatc gccaacatct tatccatttt
     4081 ccgcatgttt gttcaatgcc aatctggacc caagatggcc gaagacggat aggtgaccag
     4141 acaattgggc cttcagtgat ttggtcgccg aggacggcga ccctcccggt gaaacgtgta
     4201 ggggcaggcc cctgtacctg ccccagcagc gcagagcgcc cgcgtcaacg tcaaatgcca
     4261 acaccgccgc ccacccgtcc caacgcaaag cagtgccggc gcgattggga gggacggcgt
     4321 cccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtagggg caggcccctg
     4441 tgcctgcccg agctaaaata tgcgtctgtg ctaggtggcc aaggaatagg caagcatgat
     4501 caccttggct acaatggcta aacaaaaagc atggtcattt cgataagcaa actgcccaag
     4561 ggtttgcgtt actgcctttg ccataagttg ttttggaatc atacattgcg tctggctagt
     4621 agcgaaaaac tgattattct cggccggctg gagccttttc gggccttctg gattatcggc
     4681 gctgtctttg tcttttccat cattacgggt ggtacagcat gccagcgaca agcgagtccc
     4741 gatcacgaca gtccggcggt aaaggagtct gtcgtaagtc aggaacaagc ccaccgagag
     4801 tcgcccgctg aaccagtaaa tccaccgcaa taccgtgcgc agaagggtcc gatcgagttc
     4861 gaactcgtcc agcggctggc ctatggtacc cccgaggaag agggtacggt cccccctgaa
     4921 tttgccgaga ttattcgggg attgctggcc gcccgggcct ccctggaatt tgacaccaac
     4981 ggcaggctta ttggtgtcga tttggccggt gagcgccgca gtgggagtga cgcagagatt
     5041 tccctggccg tgcggcttcc ccatttgaag cgctttcggg tggccggctt cggcgtcacc
     5101 aacgaaggga tcaagcagtt ggcgacatgc cagaacctcg aggaacttgg gcttgagaac
     5161 acccaaatcg acgacgcagg cctggaatac ctgaaagcac ttccccgcct ttggtctttg
     5221 aatctgcgtc gatcggtcaa gcttaccgat cgtgccatcg acacgctcgt tggttttccc
     5281 cgcctgacac acctttattt gttggaaaac cagttctccg ccgaggccct gatgaaaatt
     5341 gcccaaatgg atcggctccg gctgctcgac ctgcggggtt gtggagcggt caactcggag
     5401 gtcctgcgac ggctcagcgc catggagcac ttggcggcgt tgcgtctgcg ggggtacaac
     5461 atcgacgacg tgtgcctcca ggctgtgggc ggtttttcgc acttgacaag tttcacgtta
     5521 gaagagtctc cggcgggaaa cgcgggcttg gcatcgttgg cgcggttacc gctagaaacg
     5581 gtaacgcttt ttcgttgcac cgctgtcaat gacgagggtt tgcgaatcgt ttcgcagtgg
     5641 aaaaaactcc gttccttgac gttgcgggac atggcgattc ggggagattt tttcaaggaa
     5701 ctcggcgagg tgccgcagtt gcgaagtctt tcggcgaccc agaccatggt ttccgatgat
     5761 gcgctacacc atctggtggc ttgtcaacag ctcgaggaat tggcccttcg acaaacgctc
     5821 gtcaccgaca aagggctggc ggtcataagt cgcctgccga acctacggcg actcgatgtg
     5881 gcccagacca tggtgagcga tgccggtctg gtccacctgg ccaatttgaa aagcctggaa
     5941 tggctcgact tcagcggcaa ttcgggtgta acggatgcct ctgtcaagat cctcctcaag
     6001 atgccccagc ttaaagaggt gcggctggat ggaacgtcca tgacgcaaga ggcaattgcc
     6061 caattgggac cgcgtgcccg cacggccctg ccggtagaag aataagattg atctcctcga
     6121 actataagtt ttgcctggcg gcgcgccttt tcactcatcc tccgtgacgg ggtttgacca
     6181 gggtgattcc cggcgcttgg gtttttcctt cgaatagagt tcatccaatc gtttgggatg
     6241 ggctatgaat cgggtgccca ggcgcaatcg cgcaatggtc cgcagcatca ggatggaaat
     6301 accgatcaca ctgcccgtaa tgcccatgtc gcacagcagt ccaacccaac tgagggcccc
     6361 tccactgacg aaggacacga caatccacag tgccttaagg atggccgccc cgagcaagac
     6421 ccgaaaccac agattgaggt gctgcgcatc ttcatcaagg accatctcca actcttctgc
     6481 aaatgtgagt cccgtctgcc ggacgtgggc ttcccttggt tcttcgtcga agtctttagc
     6541 tggcggcagt gaggatacag tcgccggctg ttccaccctg gcagattgga gatgacgggc
     6601 cagccgggtt tcccgcaacg gacgccactc cgacatattg accgtccata caagtgtatc
     6661 gggcggaatg agcccggtcg cggccatcgc ggccaaatgg ctttcgggga tcggtccctc
     6721 gggttcgtcg ttcaccgtgt agtaccacag actttcggac gacaccactt ccgccgtcac
     6781 cggctccggc tgttgcaaat tgggcaacac aatgtcctgg gcgtcctcgg cattcgtgcc
     6841 tcggagaacg aacacatcgt catcggcgtc attgatggtg cccaacggac cgtggctcga
     6901 aggtgaggga cctttccaag ttgacacact gtggctctgc tgcagcaacg tttccaactc
     6961 ttggatggga cgccagtttt ggccgtccag ggagatttcc cacgtggccg ccgttttccc
     7021 tttttgcacc atgcgtcgca attcgtcggc ggaatacggt ccgaagaccc gcccccggaa
     7081 gcggacgtgt atctgttgtc tggacgaatc gacgctcatg aacccggctc ctcgcccgcg
     7141 agggtcacca catgccgccc attataaaag cggacactct gctggggttc gacgacggcc
     7201 cgggagttgc tccaatgccc tatgcggcga agggtcactt ggccttcctc ggggccttgc
     7261 tccacgacat acagaacggc cccatcggga acggctggct gccccacggt ccgcacatac
     7321 cacacctgcg tcttatcttc gaagaggagc ccgaccagat caatggttgg ccacctcctt
     7381 ccccgatacg tcgcccgcac ttgcttgact tgggacaaca ggtcttctgc cgtgggcaat
     7441 gatcccagaa cgctccggat gtcttcgtac tggttctccg cggcctgggc cgtcttgatc
     7501 caatcgtaat ggggcggata acgatcccgc aggggttgca acttggcctg atagccattc
     7561 cataaagcgg cccaagcaac gtcgccctcc ttggccgccg accacgtttc gtaaaggaga
     7621 tggaagatca agggccactc aacagtatcc ttttccacag tgtgacaaaa accgatgatc
     7681 ttccccaaga ccgtgtcggc cgtggtttcc caattgctcg cagaccaatc ggccaggtga
     7741 tcgcgaacct ggttggccaa gacactttgt ggtgcccggc ctgtcgaaac aaggctttcc
     7801 cggggaaagg taatctcatt tctttgaagg ctcccaagat cgcgcaaaac cagcgcggtt
     7861 cgtcccggct gttcgaggca gtagtatcgg cgaccatcgt tcaaacgaat ctgccaggca
     7921 tcggcaacga ggggcgcccg ccactctttg acgagaaccg tcaagcggtc cgccacaagc
     7981 aattcccggc gggcaatcgt atcgagtagt gcgagaattc gtttttgtcc ctcttgaacg
     8041 aacgcggagg cctctggggg gaccacctgg acgagtcgaa acgctcctct gaacagctcc
     8101 tgggccccgg catcgctcac cgaggtggtg ggatcattcc acagcgtcgc cacacgattc
     8161 cactcggcaa tcactttcca acacggaaat tcctgttcga caagttgaaa atccccgatg
     8221 ggaattcgca cagacttggc gggctccagt ttcttgatcg cctcctggta ggcctctggc
     8281 cctcgtgcgg cagcagaaca catagtgcgg atgagttttt cctcctcgga ggcctgactg
     8341 acttcgagaa agcgacttga aagttgctcg cgcaggttac gaatctcccc ttccaagcta
     8401 agcccctgac gccgattgtc gtccgagagg acggttaggc gattcatgag acgggctatg
     8461 gcctgaagtt cctcgtcgat ggataccgac ccacgttggc gaagcgcatc atattcgcga
     8521 ctgagcttgg cgatctcctg ccgaagtcgt tcttcggcct gttggcggag gttggcccaa
     8581 atgaccttcg cctgcgattc cagctccatc acctcttgtt tctccgcatc ggtgcgggca
     8641 agttcgctgg ccttttgtag cgatacgcca tcgggaacct gagccagcga cgcccttacc
     8701 ttttccagta ggctggccaa tgtttcacgc cgcttggcct cggcagcttg ggcatcggcg
     8761 agcttttgtt cgagactgat cacgtgccga tgctgggcca gtgttgggta tgctgccttg
     8821 aagtccgcca gatacttcgc cgcctcatcg agtttgtcct tttccaagag gtcctcgacc
     8881 accgacacgg cgtcactcag tcggcggttt tccaccatcc gggcctgtac ctgccaaaca
     8941 atcaagaggg tggctatcac gaccgctgcg accccggcga cggtcaccac ccggcgaaac
     9001 atcgcccgtt gccgtaacgt ttccgtaacg cggcgatacc tttcctccaa cggctcgggt
     9061 atggccacac ctcgaatggc caactcggcg tagtgatgct cgagggtatg aaggtcatga
     9121 ctttttccga gaagggcctc aaactccgtt agcagctccc gtcgccgtcg tgcttcttct
     9181 cgctcgcgct ccagttggtg acgacgggac atggcctcgg ccacctgttc ccgcagttgg
     9241 atatctaccc attggggatt ggtcgctgct tggtgatccc aaagccgcaa gtattcctcc
     9301 atgcgaacca tgtcgccgtt ggccagggcc tgattgacga ggcgagcgat gttttccatt
     9361 tcctggcgaa aaatctttcc ccgaagcgag gccacggcag agcggcactg ttcgacgagg
     9421 gagaccgagg gcttttccag ccactcatta ctttccaatt gctcgagaat ctcctcgagc
     9481 gtttgaaggt cctctgcctt gagacgttcc tggatttccc gctggagttt atccagccag
     9541 tagttttcca gaatcctgat gtctttctgc cagacggggt tttccggctc agcccgcgca
     9601 agttgccgaa gaatggccag ccgcttgtgg agaggtgcct gggccatcgc caaggcacgg
     9661 tgttgccgca ataatccgct caggccctgt tccgcctcat atgcctgatg gagctctgag
     9721 aggtactgta cgaggaggcg cggcggcgtg ggcaggcgct ccagcgtgca gatatcaacc
     9781 caggcgtcgc ggtcgggaaa atccagaatc tccaccacat cgagcagttt cggaggtatc
     9841 tcggcgcgac ggatcgcctc gcttcgtgcc cccgaacgaa taaggtggtg aatctcctga
     9901 agacggcgat tgacctgctc gcaggcctct gcgtactcct ccgccagttg gtgcaactcc
     9961 tcgttcgaga cggcgtccaa cgaggccaat gcctgctcga tgcgatcgac aatcgtttga
    10021 tcacccgtca tcggttgccc ccaggcggtt gtgcgttttc tcgagcagat tccaccttcg
    10081 caccactgcg aatttgcaga tcaacaatgg ggcaccaaac ccgctttggt ccatcactcg
    10141 cataaaaccc aatttgagcc atcaggtcct tgggcagggg tggcccatcg ccttgtttca
    10201 cctcgtcaac cttcacagtt attttcgccg gtttgcgctc gatcttgagt ctcgcccgat
    10261 ccttaccatc gacggaaaag acgaactcag aagtcgtctg acctttcttc caaccgccaa
    10321 cgcatcgcac ctcgggtgtc attcccacca aaggaggaat ctctatcgtt ttttcctggc
    10381 ccaatgccaa ggtttctacg ggaatcgaat tcatggacag aacatagtac tgggcctggc
    10441 cattgtcatc gacgacttta aggagaaaga aatcctgggt ggactcacat tcccgactcc
    10501 ataaacgctg gctctcccaa gattgcagag ttttcacgcc cggggcgcct gttggcgtct
    10561 tttttaaaaa gattttaacg atttctttca gacgcaactc gctgattgga gccgccacga
    10621 attcatcctc tgaaactgcg ttaccctggt caggtggccc attggcagaa acaaccgatc
    10681 gctcgccgcc accggcgcgg ttggcctccc cggtagcctt tccctcggga ggcttctccg
    10741 ccgggggtgc ggaagtcatt tgatcagatt tggctgtctc acctgctggc ggccctggcg
    10801 gcttgccttg ttggggtggt tccttgggac cgacggcaac cggctgttcc ccattctttg
    10861 cctcatttcc ctgagccggg acaggtgaag gcttcctctc cgggggtttt tgtttatcac
    10921 cgcgagctgc ctgtttgaag tcgggctttt caccgcccct ttccgcggcc tggtccagtt
    10981 ctctttcggc aggattcgcg aggaaccccg gcaataatgg tcgcatgatc gtgccactcc
    11041 acagcacgaa ccccagtccg agaccgacaa taacacccgc cccaagggtg gccaggcttt
    11101 tgatcgtggc catggggacc cgccgttttc gtctttcggg gaccgctcgc ttagaggcca
    11161 cccaaagtgg tggcggggca tcgacactgt tcgccaattc aaggaaagca ggctctcgag
    11221 gcgccggtgc gggcaccgtt accggctgcg gcgttggtac ctccgacacg gggaccgaca
    11281 ccttggtcgg cgttggcaac tcgatcaagc gaccttcgcg ggccgcatcg gcttcgctag
    11341 tcgccggggc gggtccccgt ttgctccgca ggtcccacgc ctgaggaccc tgttgtttca
    11401 tgatggccgc gtactcggag gcgatctcgg gaattgcccg ccatcggcag gtgacgacct
    11461 ctcccagccc ctggtaatat gtcgaaaacg tcactttcca gcggagcgat tccggcagca
    11521 gggcaataac ttcctgaaac agtttcagca tatcgatctg gagcggatac acgaaataga
    11581 tcgtctcttg aggggcctgg agaaaccttt gggcgaggac ccccgcccag ccggcatcgc
    11641 ccaaaacctt ttgccacagt tcgcaagggc ggggttcaga ggtgattttt gggatttcct
    11701 tctccgtggg gatcacacgg agctggccgt cccacttgtc gagcatcagt tccggcctct
    11761 gaaggagttc ggccgggccg gcggggacca actcctgcgg tttgagaacc aggtggtagg
    11821 caaagcggtt ggaacgtccc gaataatcgc gtcccgcatc gcaaatccgc gaaaggacat
    11881 gaaacgtctc gcggccaaga cggaggaccg tgtgaccata aacgacagga ttgcgcgatt
    11941 cattgcccgc accgatctcc aggtgacggt agccgctgat cttctccagc cgttcgatca
    12001 gttgaggtgg catcccctgc gtatgcgcca caggacaaaa tccggcgcgg cccgggtgca
    12061 aaccacgcgg cgctgaggtg atgctcagtt cccacgccat gataatgaac ctcctcaggg
    12121 gcttctgcgc ttaagtggcc tttcagagat cggtgctcta ttgcctcttt ctcatgtcac
    12181 tcctggctca cgacactc
//
LOCUS       DAOPTR010000003        11782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_4596_length_11782_cov_3.538842, whole genome shotgun sequence.
ACCESSION   DAOPTR010000003 DAOPTR010000000
VERSION     DAOPTR010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 11782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11782
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4596_length_11782_cov_3.538842"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /locus_tag="PKI05_00085"
     CDS             <1..1432
                     /locus_tag="PKI05_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOA50619.1"
                     /translation="KTKTTSPEHQFANQLSAAFRAAAQEVLPSVVLIRTYAKVGGRSE
                     EIIPFFDNPFEGTPFEDMFRDPSFRQFFRRGPTPPQFVRGLGSGVIIDPAGVILTNAH
                     VVTPEGKILVRLHDGREFDAVDVKTDPKTDLALVWIKGAKGLKAAVLGDSDQVQVGDW
                     VLALGQPFGLEGTVTAGIVSAKGRGLGIAFRENFIQTDAAINPGNSGGPLVNLDGEVI
                     GINTAISTESGGYQGVGFAIPSNLAKFVATQLAEKGFVRRGYLGVAIQPVEGALAEQL
                     GVEPNEGVLVTEVYPNTPASEAGIKSGDVILSFDGKKVSSPNELQSYVEESEIDKAHD
                     VEILRDDKRMTIKVTVREQPSEYGMDRVPPRLRGRGRALPESEAIAKLGIQVETLTPE
                     VAEQLGTDLKEGVVITAVKPGSPAALAGLSEGTIIVEANRQKVSNVSDLREALAAKPL
                     SQGVLLRVATPEGMSRYVAIRVGENE"
     gene            complement(1674..2843)
                     /locus_tag="PKI05_00090"
     CDS             complement(1674..2843)
                     /locus_tag="PKI05_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOA50620.1"
                     /translation="MPREKMTSRERLLAALNHQEPDRVPIDLGGNQTGIHRLAYEALL
                     KHLGWEETIEIMDAVQQLARPSEAVLERFHVDTRYIAAKPAESWKGEIVRAERDGRVW
                     LDLVDEWGVRWSMPEDGGLYMDITYHPLAKATMKDLQEYPFPKGDDPSRFVGLREEAL
                     RIRRETPYAVVSGISGVVYETCWYLRGLERWFIDMIEQPEFCAALLDRTLRFWMDWFR
                     LFLKEVGDLVDVIMIGDDLAGQDGPLFSPSFYQAIVKPRHKALVQFIRSQTHAKIWYH
                     TCGACRSFIPDLIDNGIDILNPVQISARDMDPTTLKAEYGQQLAFWGGAIDAQHVLPS
                     ASPDEIREHVRRHLEIWKPGGGYVFNNVHNIQAGVPPENIVALFDAAYEFGFYEQ"
     gene            3134..4585
                     /locus_tag="PKI05_00095"
     CDS             3134..4585
                     /locus_tag="PKI05_00095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-efflux pump"
                     /protein_id="HOA50621.1"
                     /translation="MHVEFHGKTQVALASLLAAAGLTAGKVVVGILTNSLGIISEALH
                     SGLDLVATGMTLWAVRIADRPADEEHRYGHGKFENLSALAETALLLLVCVWVFYEAIQ
                     RLFFRAELEILPSPWAFGVVIASIVVDLWRAHHLREAAEKYSSQALEADALHFASDVW
                     SSLVVLLGLTCVLLAQRLDWPWLIHADALAALIVAILVSVICVRLGLRAIDALTDRTS
                     RSLAHLVEHSASQVKGVEEVRDLRVRQSGAGVFADMTVCVPRELGLAQAHEVATNVTA
                     AIRSQLPEADVVVHVEPVAREDEDLVTSIRALAASLGLAVHDVQVISTPVGNSIELHV
                     EVSPDLNLEQAHDLVTRLESTIKSDLPKIESVLTHIEPNGVTRAESEARDQAPIRTES
                     FHQAIADFSQASGTPMNGHALVTRQTPQGVSLSFHLHLDGTLPIGTAHEISRTLESYL
                     HRKFPELSRIVIHIEPHPPAEGHSPEPGPPQVS"
     gene            complement(4603..8160)
                     /locus_tag="PKI05_00100"
     CDS             complement(4603..8160)
                     /locus_tag="PKI05_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HOA50622.1"
                     /translation="MATTEVTVSFHGLSRELQIPEERIQAVVQLLDAGNTVPFITRYR
                     KDQTGGLDEEQIRTIQNRVTKLRQLAERKQAILRAIEAQNKLTPTLAKKILAANSLKR
                     LDDLYLPFRPKKQTLATIARERGLGHLAEEILNADPICADLDKRAADYINPDKQILSG
                     ADALLGAGHILAEMFSENAALRARLRDILKKTGVIKTVRIAPEVSATPQQPSPAGQSP
                     TPSPATELPTPASESSVVQAVTTPPEEQTAAAGSSLASEPASETAATEPDQTGSIPTE
                     AAPGETEDLPLAGSREGMERPSEAASPAGETEQRLEAQASEHPNAENTETGEAAPIAE
                     SPSGPAEVQQPTDAMPPAASSGESSQQAATATSPPTNAAKTPNPRAAAKEAEKRRKEE
                     QRIKEFADYFDFQEAIRSIPPHRVLAINRGEALKIIRVKIKADEEALDRAVEEICVPK
                     DHPHADFLRGCARDALNRLILPSLEREIRRELTEKAEAHAVKVFAKNLRRLLLQRPVR
                     DRRVLAIDPGYRNGCKLAALDEYGNVLDHKLIYLVEKKGYTREMAKETVCELIDKYQL
                     TCIAIGNGSGSRLAEMFIAELLENELKNRGIGYTIVNEAGASVYSTSQIGREELPNLE
                     ASYRGAVSIGRRLLDPLSELVKIDPANLGVGMYQHDLKEKHLRESLDEVVESCVNYVG
                     VDVNTASPALLGYVSGLNKLTARRIYEYRQEHGAFRSREELKKVPGIGEATFVQAAGF
                     LKISGGDNPLDATWIHPESYELAHRVMEKLGITAEDLTKADKRAELAARINQVNVEAL
                     AAELNCGVFTLRDILAQFVRPGRDPREELPPPVFKQGILKLEDLSPGMELMGTVLNVV
                     DFGAFVDIGLPNSGLVHRSRMDRNFVADPHSVVAVGDVVRVWVVDVDRDRRRISLSLV
                     PLEELAEKQVSRERRAERTAEKPRRQKAEKPRRTAATPSDETEAQKREESAEGQRERR
                     ARFPRREDRPARPEESVAKSQDGMPVISRPVRLPRRPEREQPKAPAEPLSEEVLTGKE
                     PMRSFADLMQYFAAKGVIRPSTPKTSGSHSGRSGKKNKGGKDKKGGRSSEDRRTPEQA
                     APSPADRLGDQPKPAESPSPTDHQQREQPTSEGTSIPNAPAQSQDTITGNPRNQGVES
                     AGTIPVPDEGQVPGPDAIAPAEEAGSSEGENQQKSSDGP"
     gene            8893..9057
                     /locus_tag="PKI05_00105"
     CDS             8893..9057
                     /locus_tag="PKI05_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50623.1"
                     /translation="MEGISGWDIALWSVAAFAAVMLLVRVMLSFRQYWVEYYRMQAEA
                     ARKRKPKKEG"
     gene            9158..10561
                     /gene="miaB"
                     /locus_tag="PKI05_00110"
     CDS             9158..10561
                     /gene="miaB"
                     /locus_tag="PKI05_00110"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOA50624.1"
                     /translation="MGKRFYIETVGCQMNELDSELVIAALLEAGYEYVPRRQDADIIL
                     FNTCSVRQHAEDKIYSALGRLKHLKEQKPGKIIGVLGCMAQKDQGLILKRAPHVDLVV
                     GPGQVARLPELLQKLGESGSPIVEVSLDRRGVSQQRVRASFLPLDRKERPSVRVTPYQ
                     AYVRVMYGCDKFCTYCVVPRVRGPEQSRSPSEILDEVRQLADQGCLEITLLGQTVNSY
                     HWTEGGKTWRLSDVLAEVHEVEGIRRIKFVTNYPCDMTPDLLQAVRDLPKVCPYLHVP
                     AQSGSNRILQRMKRGYTVEEYREMLAAIRETIPGAAITSDFIVGFCGETEEDFAATVK
                     LVEDTRFKNSFIFKYSPRPGTKAAELWPDDVPEEVKRDRNNELLAIQNRIAEEENRRF
                     VGQVVEVLVEGPSKASGKQRQSDGSLQLTGRTACDRIVVFPGSSDLVGQFVSVKIEAV
                     SAVTLFGHVLAAEAAVS"
     gene            10680..>11782
                     /locus_tag="PKI05_00115"
     CDS             10680..>11782
                     /locus_tag="PKI05_00115"
                     /inference="COORDINATES: protein motif:HMM:NF015655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50625.1"
                     /translation="MSGLRLRDPRVAHRNLLSIARQGVPLDLLTVLIQQMEKVLPELA
                     DPDMALNNLERFFAATRNPLSLASLFERDPEALAPLLQIFSASQFLSEILICDPESYD
                     LLRLTEGQPVSRQALIDELTSEIAALTHENEIMATLRRFKRREILRIAYGDFVCGHSL
                     STVTRQISYVADALLEAAYQAARKKLRGKLGEPRTVDGSVARFVILAMGKLGGVELNY
                     SSDIDLIFLYEDEGTTDGPRSVSNAEYFHRLVQEIVRFLTLRTELGAVYRVDRRLSPE
                     GEQGPAAISVPAALRYYDMRGRTWERQAYIKARPAAGNLDLGYAFLEQLEPWIYRRYL
                     TRADISGIKTLRRKIEQRARAQGDLRDVKNGSGG"
BASE COUNT         2442 a         3340 c         3342 g         2658 t
ORIGIN      
        1 aaaaacaaaa accacttccc cggaacatca atttgctaat caactgtcgg cggcatttcg
       61 tgcggccgcg caagaggttt tgccaagtgt tgtgttgatc cgcacctatg cgaaggtcgg
      121 gggccgcagt gaggagatca tacccttctt cgacaatcct tttgaaggga caccttttga
      181 ggatatgttt cgggaccctt catttcgcca gtttttccgt cgcgggccaa cgccaccaca
      241 atttgtacga gggcttggct ccggtgtcat cattgatccc gccggcgtca ttctcaccaa
      301 tgcccatgtg gtgaccccag aggggaagat cctcgttcgg ttacacgatg ggcgagaatt
      361 cgacgccgtt gatgtcaaaa ccgacccgaa gacggatttg gcccttgtct ggattaaagg
      421 tgccaagggt cttaaggcag ccgtattagg cgacagcgat caggttcagg tgggcgactg
      481 ggttcttgcg ctcggtcaac cttttggttt ggagggcacg gtgacggcgg gtattgtaag
      541 tgccaagggc cgtgggttag gcatcgcctt ccgcgagaac ttcattcaga ccgatgcggc
      601 catcaatccc ggtaacagtg gtggtccgct ggttaacctt gatggtgagg tcatcggcat
      661 taacacggcc attagcaccg aatctggcgg gtatcaagga gtcggtttcg ctattccgtc
      721 aaatcttgcc aaatttgttg cgacgcagtt agcggagaaa ggtttcgtcc gccggggtta
      781 cctaggggta gcgattcagc ccgtcgaagg tgccctggcc gaacagttgg gcgtggaacc
      841 caacgaagga gtcttggtca ctgaagttta cccaaacact ccggcatctg aagccggcat
      901 taaatcgggt gacgtgatct tgagtttcga cggcaagaaa gtcagctcac caaatgagct
      961 tcagagctac gtggaggaat cagagatcga caaggcccac gatgtggaaa tcctccgcga
     1021 tgacaagcgg atgacgatca aagttactgt ccgcgaacag ccgagtgaat acggcatgga
     1081 tcgtgtgccg cctcggctcc gcggtagagg tcgcgcgctc cccgagtctg aagccattgc
     1141 caagttgggc atccaggtgg aaacgcttac gcctgaagtg gccgagcaac tgggaactga
     1201 tttgaaggaa ggagtcgtga ttacagcggt gaagcctggc agcccggcag ccttggccgg
     1261 attgtctgaa ggcaccatca tcgttgaagc caatcggcag aaggtgagca acgttagcga
     1321 cctgcgagag gccctggcgg ccaaaccgct tagccaaggg gtgctcctgc gtgtggcgac
     1381 gcccgaaggc atgtcccgct acgttgccat tcgcgtcggt gaaaacgaat aagagtctat
     1441 cccagtaagt ccccctctcc ttccgggaga gggttggggt gagggcaagt gatcgtcgga
     1501 gttgatccag aaaaccggcg ccgattcgag ccaccctcaa ccctgccccc tctcctgccg
     1561 aactggtgag gggagatgat gggataggct ctaagacccc cgattatcgg tggcgatgag
     1621 gttctggcgg cccaaggctg ataacaggca atcttgggcg gcttttttct atttcattgc
     1681 tcgtagaacc caaattcata agccgcatcg aacagggcca cgatattctc gggcggaaca
     1741 ccggcctgga tgttgtgcac gttattgaat acgtaaccgc cgcccggctt ccagatctcc
     1801 agatgacggc ggacatgttc gcggatttcg tcgggagagg ccgacggcaa tacgtgttgc
     1861 gcatcgatgg ccccacccca gaaagccaat tgctggccgt attcggcctt gagcgtggtg
     1921 gggtccatat cccgcgcgct gatctggacg ggattgagga tatcgatgcc gttgtcgata
     1981 agatcgggaa taaagctccg gcaggcgccg cacgtgtgat accatatctt ggcgtgcgtc
     2041 tgcgaacgga tgaattgaac gagggcttta tgccgcggtt tgacgattgc ttggtaaaag
     2101 ctgggactaa agagtggacc atcctgcccg gccaggtcat caccaatcat gatcacatca
     2161 accaggtcac cgacttcctt taagaacaag cggaaccagt ccatccaaaa ccgcagtgtg
     2221 cgatcgagta aggcggcaca gaattctggt tgctcgatca tgtcgatgaa ccaccgttcc
     2281 agtccccgca ggtaccagca ggtttcatag accacgcccg agatgccgct gacaacggcg
     2341 taaggagttt cccggcgaat gcggagtgcc tcctctcgta atcccacaaa ccgagacgga
     2401 tcatctcctt tgggaaaagg atactcctga agatccttca tggtggcctt ggccaaggga
     2461 tgataggtga tgtccatgta aagaccaccg tcttccggca tggaccaacg gacaccccat
     2521 tcatccacga ggtcaagcca aactcgccca tcgcgttctg cccgcacaat ttcgcctttc
     2581 cagctctccg ccggttttgc cgcgatatag cgggtatcaa cgtgaaagcg ttcgagcacc
     2641 gcttccgagg gacgggccaa ctgctggacg gcgtccataa tctcgatcgt ctcttcccat
     2701 cccaagtgct tcaacaaggc ctcgtaggca aggcgatgaa tacccgtctg gtttcctccc
     2761 aggtcaatag gaacacggtc cggttcctgg tggttgagcg ccgccaaaag ccgttcccgc
     2821 gatgtcattt tctctcgtgg catagggtgt atctttcttg gttaagagtc ctcggatgtt
     2881 cgaccacaaa ccgactcttt cgtctggatg atcccccgtc aaacgatgct cgaagatttt
     2941 ccagcacgaa atacccgtgc tttcgttttt gggtaatgtg agcaaggctt gtttgatgaa
     3001 gataacgcaa tggcagaccg cgaacaacta ccgagttcgt atgcgacaaa cggacgtata
     3061 atgagaagtt tgaacggccc caagagcgaa cccgctagac aatcccttgg cctatcaggt
     3121 atcacgaagc actatgcatg ttgaattcca cgggaaaacc caggttgcct tggcgtcact
     3181 tttagcggcc gctggtttga cggcagggaa ggttgttgtc ggtattttga caaacagcct
     3241 gggaattatt tccgaggccc ttcactcggg cttggacctg gtggctacgg gaatgacgct
     3301 ctgggccgtc cgcattgccg atcgaccggc cgatgaagag catcgttacg gtcacgggaa
     3361 atttgagaat ctttctgcct tggccgagac ggccttattg ttgctggttt gcgtttgggt
     3421 tttttatgag gccattcaac ggcttttctt tcgagcagaa cttgaaatcc tccccagccc
     3481 ctgggcattt ggcgtcgtca ttgcctcgat tgtcgtcgac ctttggcggg cccatcactt
     3541 gcgggaagct gctgagaaat attccagcca ggccctggaa gctgacgcct tgcattttgc
     3601 ctccgacgtt tggtcatcgt tggtcgtctt gttggggttg acctgtgtcc tgctggctca
     3661 gcgattggat tggccctggc tcatccatgc ggacgccttg gcggccctca ttgttgccat
     3721 cttggtttcc gtgatctgcg tgcgactagg actacgtgcc atcgatgcct tgaccgatcg
     3781 gacatctcgc tcgcttgccc atctggtgga gcattcggca agccaagtga aaggggttga
     3841 ggaggttcgc gacctccggg ttcggcagag tggggccggc gtgtttgccg atatgactgt
     3901 ttgcgtcccc cgcgagttgg ggctggctca agcccatgag gtcgccacca acgtaacggc
     3961 tgccatccga agtcaacttc ccgaggcgga tgtcgtagtg cacgtggaac ccgtggcgag
     4021 agaagacgaa gaccttgtca catccatccg agccttggcc gccagtttgg gacttgctgt
     4081 gcatgacgtg caggtcattt ccactcccgt gggaaactca atcgaattac atgtggaagt
     4141 cagccctgac ttaaacctgg aacaagccca tgacctcgtc actcggcttg aatccaccat
     4201 caagagcgat ctcccaaaaa tcgaatctgt cctaacgcac atcgaaccga atggcgtgac
     4261 cagagcagaa agcgaggctc gcgatcaagc gccgatcaga acggaatcat tccatcaggc
     4321 cattgccgat ttcagtcagg cctctgggac acccatgaat ggacacgccc ttgtgacacg
     4381 tcaaacaccg cagggggttt ccctgagctt tcatctgcac ctggatggca cgctgccaat
     4441 cggcacggct catgagatca gccggactct ggagagctac cttcaccgga aatttccgga
     4501 attgtcccgc attgttatcc acattgagcc ccaccctccg gcggaagggc attcgcccga
     4561 accaggccca ccacaggtat cctgaagtcg cctaactctt gatcacggac catctgaact
     4621 cttttgttga ttctcacctt cagaagaacc agcctcttct gccggagcaa tcgcgtccgg
     4681 gccgggcacc tggccctcgt caggcacggg aatcgtccca gcagactcga caccctggtt
     4741 tcgcgggttt cctgtgatcg tatcctgcga ctgtgctgga gcattcggga ttgaagtccc
     4801 ttcacttgtg ggctgctcgc gctgttgatg atccgtcggc gacgggcttt cggcgggctt
     4861 tggttgatcc ccgagacggt ccgcaggcga aggagccgct tgttcggggg ttctgcggtc
     4921 ttcggaacta cggccgccct ttttatcttt gccgcctttg ttcttcttcc ctgatcggcc
     4981 actgtggctt cccgaggtct taggcgtgct gggacggatc accccttttg ccgcaaaata
     5041 ctgcatcaag tcggcaaagc tccgcatcgg ttccttgccg gtcagtacct cctcgctgag
     5101 cggttccgcg ggggccttgg gttgctcgcg ttcaggacgc cgtggcaatc tgacgggtcg
     5161 cgaaataacg ggcattccgt cctgggattt cgcgacactt tcttctggcc gggcaggccg
     5221 atcctctcgg cgagggaatc gagcccgccg ttcccgttgg ccttccgctg attcctcacg
     5281 cttttgcgcc tcggtttcgt ccgacggggt cgcggccgtt cgccgtggct tttccgcctt
     5341 ttgccgtcga ggcttttccg cggtgcgctc ggcacgtcgc tcgcgcgata cctgtttctc
     5401 cgccaattcc tcaagtggga caaggctcaa cgaaatccgt ctccgatccc gatcgacgtc
     5461 caccacccaa acgcgaacca catcacccac agccacgacc gaatgcgggt ccgccacaaa
     5521 attgcggtcc atccgactgc ggtgaaccag gccgctgttg ggtaaaccaa tatcgacaaa
     5581 ggccccaaaa tccaccacgt tgaggaccgt tcccatcaac tccattcccg gcgaaagatc
     5641 ctccagcttg aggatcccct gtttgaagac aggtgggggc aattcttcac gtggatcacg
     5701 ccctgggcgg acaaactgtg ctaggatatc ccgcagggta aagacaccgc aattcaactc
     5761 ggccgccaga gcttccacgt tgacttgatt aatgcgggcg gccaactcgg ctcgcttgtc
     5821 ggcctttgtc agatcctcgg ccgtgatccc cagtttttcc atcacccgat gggctagttc
     5881 atagctttcc gggtgaatcc acgtggcatc taggggattg tcccctcccg aaattttcaa
     5941 gaatcccgcg gcctggacaa acgtggcctc tccaatgcca gggacctttt tcaattcctc
     6001 acggctgcgg aaggccccat gttcctgacg gtactcgtag atccgtcgag ccgtgagctt
     6061 attgagcccc gatacatagc ctaacaacgc gggactggcc gtattgacat ccacgcccac
     6121 atagttgacg cacgattcga cgacttcatc caacgactcg cggaggtgtt tctccttgag
     6181 atcgtgctga tacataccga cgccgaggtt ggccgggtca atcttcacca attcactcag
     6241 cggatccagc aggcgtcgcc caattgatac ggcgccacgg tacgacgcct ccagattggg
     6301 gagctcctct cggccaattt ggctcgtcga atacacgctg gctcctgctt cgttgacaat
     6361 cgtgtaacca atcccccggt ttttcagctc attctccagt aactcggcga tgaacatctc
     6421 agccagtcgc gagccgctcc cgttcccgat ggcgatgcag gtcaactgat acttgtcgat
     6481 cagttcacag acggtctcct tggccatttc ccgggtgtaa cccttcttct cgaccaggta
     6541 gatgagctta tgatcgagaa cattgccata ttcgtcgagc gctgcgagct tgcacccgtt
     6601 gcgataaccg gggtcaatgg ccagcactcg gcggtccctc accggtcgtt ggagcaaaag
     6661 tcgccgcaga tttttggcaa agaccttgac tgcgtgagct tcggcctttt ccgtaagttc
     6721 gcgccgaatt tccctttcta aactgggcaa aataagccga ttgagggcat ctcgggcaca
     6781 cccgcggagg aaatctgcgt ggggatggtc tttcgggacg caaatttcct caacggctcg
     6841 atcaagggcc tcttcgtccg ccttgatctt gacccgaatg atcttgaggg cctcacctcg
     6901 gttgatggcc aggacccgat gaggaggaat cgatcgaatg gcctcttgaa aatcgaaata
     6961 atcggcaaat tccttgatcc gctgttcctc tttgcgacgc ttttcggcct ctttagccgc
     7021 tgcccgcgga tttggcgtct ttgccgcatt ggtcggcggg gaagtcgcgg ttgccgcctg
     7081 ttgcgatgat tctcccgagg aagcggccgg gggcatcgca tcggtcggtt gttgcacttc
     7141 ggcgggacca cttggggatt cggctatggg agctgcctcc ccagtctcgg tgttttcggc
     7201 attaggatgt tcagacgctt gcgcttcaag ccgttgctca gtctcccccg caggagaagc
     7261 tgcctctgac ggccgctcca ttccttccct gctccctgcg aggggcaaat cctcagtttc
     7321 cccgggcgcc gcctcggtgg ggatcgatcc cgtctgatcg ggttcggtag cagcggtctc
     7381 ggaagcaggc tcgctagcca acgatgaccc ggccgcggca gtttgctcct ccggtggggt
     7441 cgtcacagct tggacaactg agctttccga ggccggtgtc ggtagctctg tggccggact
     7501 gggagttggg ctttggcctg cgggagaagg ttgttgcgga gtggctgaga cctctggggc
     7561 aatgcggact gtcttaatga ccccggtctt cttgaggata tcccgcagtc tggcccgcaa
     7621 ggccgcgttt tcgctaaaca tctcggccag gatgtggcct gcccctaaga gtgcgtcggc
     7681 cccggagagg atttgcttgt ccggattgat atagtcggcc gcccgcttgt cgaggtccgc
     7741 gcagatcggg tcggcattaa ggatctcttc cgcgagatgc cccaaacccc gctcacgagc
     7801 aatggtggcc aaggtttgct ttttggggcg gaagggaaga tagagatcat ccagtcgttt
     7861 caggctgtta gcggccaaaa tctttttggc cagggtgggt gtgagtttgt tttgagcctc
     7921 aatcgcccgg aggatcgcct gcttgcgctc cgcgagttgc cgcagcttcg tgacacggtt
     7981 ttggatcgtc cgaatctgct cctcgtcgag tcccccggtc tgatccttgc gataccgcgt
     8041 gatgaagggg accgtgtttc ccgcgtcaag cagttggaca acagcctgaa tccgttcctc
     8101 cggaatctgt aattcccgtg acaatccgtg aaaagatacc gtaacttcag ttgtggccat
     8161 aagctctcgg gcgattgtct ttccaaagcg ctcgcgtgct tcgacaccga gaatcctcca
     8221 ccttgggata cccaggagga gactcccgcg gtgggacgaa cttaggacca gtgctcctga
     8281 ggccaggtgc cctggtcacc gagactcggt aaccctgtca tacgatctga tattcagata
     8341 caacgcgtcg cgcaacctcc tcaacgtccc tctcacaaat aaccgaattg actgccaaaa
     8401 atctctctac cccaactgat tgagtataac cgatttggtg aggttctggg tacgggaagt
     8461 ggcttagcct gtttcaagaa aaaacacgac accacccttt agaaggtcgc gttgttgtgt
     8521 ctggctccag gtccaatcaa gttcccaaaa cagcgaaatc aacccgtccc tacccggagt
     8581 cgtcccttta agagcttatc cgcaaaagtc tccctctccc tccgggagag ggctggggtg
     8641 agggcaagcg aacgtcggag tgggtcggaa aaaccagcag aaattcgcgc cactctcacc
     8701 tccgccccct ctcccgccaa acgggcgagg ggagattatg gagtggacgt taagtacggg
     8761 atcatcacag ccggggcata ccaaatatta tcctcgattg cccacgggaa ggcgcggaac
     8821 aaggcctttc cggtaaaata gaggaaacag cattcggcag atcggccagt tttcccagga
     8881 gcatccgagc gtatggaagg tatcagtgga tgggacatcg ccctttggag tgtggcggcc
     8941 ttcgctgctg tgatgctctt ggtgcgggtc atgctgtcat ttcgacagta ctgggttgag
     9001 tattaccgga tgcaggccga ggcagctcga aagcgaaaac cgaagaaaga ggggtgaacc
     9061 cgggtctctc gcgtcggccc caaggctgat ctccgtttgt ggaccccaca gcatctgacc
     9121 gccttttact cgatcatgtg aacgtctgca caacacaatg ggaaaacgtt tttacattga
     9181 aaccgtgggc tgtcaaatga atgaacttga cagcgaactc gttattgcag ccctcctcga
     9241 agcgggctac gagtacgtcc cccgccggca ggatgccgat attatcctct tcaacacgtg
     9301 tagcgtccgg cagcacgccg aagacaaaat ctacagtgcc ctaggtcggc tgaaacacct
     9361 gaaagagcaa aaacccggaa aaatcatcgg cgtcttgggc tgtatggcgc aaaaggatca
     9421 aggtttgatc ctcaagaggg cgcctcatgt ggacctggtc gtaggcccag ggcaagttgc
     9481 ccggttaccc gagctcctgc aaaagctggg cgagtccggt tctcccattg tggaagtcag
     9541 cctagaccgc cgcggggttt ctcaacaacg agttcgcgcg agtttcctcc ccttagatcg
     9601 caaggagcgt ccgtctgtcc gcgtcacgcc ctaccaggct tatgtgcggg tcatgtacgg
     9661 ctgcgacaag ttttgtacgt attgcgttgt gccgcgagta cggggtccag aacaaagccg
     9721 atcccccagt gaaatcttgg atgaagtccg ccaattggcc gatcaagggt gccttgagat
     9781 cacgctgctg ggtcaaacgg tcaacagtta tcactggacc gaaggcggga agacctggcg
     9841 gttgtccgat gtcctggcag aagtccatga ggtagaaggc atccgccgga tcaagttcgt
     9901 gacgaactat ccctgcgata tgacgcccga tctcctccaa gcggtgcgcg accttcctaa
     9961 agtgtgtccg tatttgcacg ttccggccca aagcggttcc aaccggattc ttcagaggat
    10021 gaagcgaggg tacactgtgg aggagtatcg agagatgctc gcggccatcc gggagacgat
    10081 cccaggcgcc gccataacga gcgactttat tgtcggtttt tgtggcgaaa cggaagaaga
    10141 ttttgccgcc accgtcaaac ttgtggagga tacccgcttt aagaacagtt tcatcttcaa
    10201 gtacagtcct cggccaggaa cgaaggccgc cgaactttgg ccggatgatg tgccagaaga
    10261 ggtcaagcga gatcgcaaca acgagctttt ggctatccag aaccgtatcg cagaggaaga
    10321 gaatcgccgc tttgtgggac aggttgtgga ggtgttggtg gagggcccca gcaaggccag
    10381 cggcaaacag cggcaatcgg acggttcact acaattgaca gggcgcacgg cgtgtgatcg
    10441 gattgtggta tttccggggt catccgacct ggtgggtcag tttgtttccg taaagatcga
    10501 ggcggtgagc gccgtaacat tgtttgggca tgtgctggcg gcggaagctg ccgtctcttg
    10561 aaatgaattc cggaccgtcg cggcctggaa agccatcctt tgcagcaaat ctgacattgt
    10621 ttatggaagt cgaaacgtta cgttcgtttt tggagaaccc tgaaggagcc aaccgttggt
    10681 tgtcgggcct tcgtctgcgt gatccccgtg ttgcccatcg gaacctcctc agcatcgccc
    10741 ggcagggagt cccgctggac ttgctgacgg tgctcatcca gcagatggaa aaggtgcttc
    10801 cggagttggc cgacccggac atggcgctca ataatttgga acgttttttc gccgctaccc
    10861 gcaatccgct gtcgttagcg tcactttttg aacgtgatcc cgaggccctc gcccctttgc
    10921 tgcagatttt ctcggccagt cagttcctta gcgaaatcct gatctgtgat ccggaaagct
    10981 acgacctgct gcggctgacc gagggccaac ccgtttcccg ccaggccttg atcgacgagc
    11041 taacatcgga aatcgccgcg ctgacccacg aaaacgagat catggccacg ttgcggcggt
    11101 tcaaacgacg ggaaatattg cggattgcct acggggattt cgtctgtggt cacagccttt
    11161 ccacggtcac acggcaaata tcgtacgtgg cggatgccct gttggaagcg gcctaccagg
    11221 ccgcccgaaa aaaactccgc ggcaaactgg gagagcctcg cacggtcgat ggctcggtcg
    11281 cccgtttcgt catcctcgcc atgggaaaac tgggaggagt tgaactcaat tattccagcg
    11341 acatcgatct gattttcctc tatgaggacg agggaacaac cgatggcccg cgttccgtga
    11401 gcaacgccga atacttccat cgactggttc aggaaatcgt gcgctttctc accctgcgga
    11461 ccgaattggg ggccgtttac cgtgtcgatc gtcgcttaag tccggaaggc gaacagggac
    11521 cggccgccat cagtgtaccg gcagctcttc gttactacga catgcgaggg cgaacatggg
    11581 aacgacaggc ctacatcaag gcgcgaccag cagccggaaa cctagacctt ggatacgcct
    11641 tcctggaaca actagaaccg tggatttatc gccgctattt gacgcgggcc gatatttcgg
    11701 gaatcaaaac tcttcgccga aaaatcgaac agcgagctcg ggcccaaggc gatctccgcg
    11761 atgtaaagaa tggttccggc gg
//
LOCUS       DAOPTR010000004        11297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_4835_length_11297_cov_3.560043, whole genome shotgun sequence.
ACCESSION   DAOPTR010000004 DAOPTR010000000
VERSION     DAOPTR010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 11297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11297
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4835_length_11297_cov_3.560043"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1555)
                     /locus_tag="PKI05_00120"
     CDS             complement(68..1555)
                     /locus_tag="PKI05_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA50626.1"
                     /translation="MFARWGGVLLAVWSLGIVFVGWPQGLACEPGPELRTSTPSVERP
                     NLLVIITDQQHAGMLSCAGNPYLRTPAMDSLAAQGARFELAYTANPVCIPARMSMVTG
                     RMPSAFQIRSNAEGKNPAPKEAIDQAMGWIFRRAGYKTFYGGKTHWMRGMTPQSTGFG
                     ELTKDTREELARKCAEFLSEPPAEPFLLVASFINPHDICLMAIDAFTQATGRPAIYPE
                     LQRDRECLAEALALPAGISEAEFFDRLCPPLPNNFEIPSNEPDGVTLSYLPPQSFRAY
                     VRQHWTERDWRLHRWAYCRLTERVDAEIGIVLDALRQHGLDRKTVVIFTSDHGDLDAA
                     HRLEHKSIPYEEAVRVPFIVAWPGVTKPGLVDRTHLVSVGLDLIPTLCDFAGIEKPPH
                     LLGLSVRPLAEGRTVDTWRESVVVESDAARMIRTARYKYVVYEKGTRREQLIDLKNDP
                     GEMENLAGQSAYDQILKEHRRLLWEWVTRIGDTIGQSFVIAPDER"
     gene            1840..2784
                     /locus_tag="PKI05_00125"
     CDS             1840..2784
                     /locus_tag="PKI05_00125"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOA50627.1"
                     /translation="MHRVALLGHFIVYVIVRFLIFVVQMIPLAVGSWLAWCLAYLFTY
                     VLRIRYSVADENLRYAFAELSPSQRRDLILRMWHHLFLLVMEVAHTQAKIREENWRQR
                     ATLKNVEPLVKLLLEDRPVIIVTAHFGNFELGGYMLGLLGFPSATIARNLDNPFLDRF
                     VQRFRAARGQYLIPKVGGADQVEEMIQKGHTIAVLADQDAGRKGCFVPFFGRPASTFK
                     AIALLSLEYDAPIAVVYARRTGKPLHFEMGLVGILDPRDVSAPDAVTFVTQWYTSRLE
                     EMIRSAPEQYWWIHRRWKTQPKSPKLRIPSEQAVRSAA"
     gene            3075..4316
                     /locus_tag="PKI05_00130"
     CDS             3075..4316
                     /locus_tag="PKI05_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOA50628.1"
                     /translation="MTPKQRVLAALEHHEPDRIPIDFGGHRSSGIAAIAYARLRKYLG
                     LPERPIRVYDPIQQLAIIDEDVLNRFRVDTIELGRAFAQEDRYWVDWVLPDGTPCQMP
                     SWAVPERSDGGWVLRGPTGRILGRMPDGALYFEQCYWPFAEKDQPERAASALIDTIWC
                     GIPSPPGPIVEGPGGLDRLREGARRLRQQTDRAILFLFGGNLLECGQFLYRNDNFYML
                     LAGDRPRAEKVLDSLVEAHLANLERVLPVLDGLIDIIVFGDDLGMQSGPQISPKMYRE
                     LFKPRHAAMWKKAKELADVKVMLHCCGGVRELLDDLIDAGLDAINPVQISCKGMNPTE
                     LKRDFGKRITFWGGGCDTHRVLSFGTPEEVKQHVREQIESFAPGGGFVFQQVHNILAN
                     VPPENIVAMFDAVYEFGAMYR"
     gene            4514..5701
                     /locus_tag="PKI05_00135"
     CDS             4514..5701
                     /locus_tag="PKI05_00135"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOA50629.1"
                     /translation="MSRFSWRLLRRVSPRLLLKAGRLWLIPGLRSLSRFRKRVAASEL
                     FPPFFFLSLTDACNLRCHGCWITSQGQPRQVSRDDVNHFIAQAKVHGNRFFVLLGGEP
                     FLYKDLWTILRQNRDCYFQIITNALLLDEAAVATAKEVGNATILVSLDGFESANDARR
                     GQGTFQAIKEAFQRLRKGKILFGVATTITSENYEAALSPEYVNWVDEQGAIYLWYYIY
                     RPVGPDPMPQLALSRADILKVRRQLLRLRRISPILIVDTYWDADGKAVCPAALGLGYH
                     IGPAGDIELCPPLSFARETIRDHGGDVFHTINTSDFLRGFQKFALSRTRGCPILECPH
                     ELEAFLREHHARDTSGRDAFAELRASSPRASHHIPGEELPETMGTYRWLKKHLFFGLG
                     AYG"
     gene            5805..6293
                     /gene="sixA"
                     /locus_tag="PKI05_00140"
     CDS             5805..6293
                     /gene="sixA"
                     /locus_tag="PKI05_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HOA50630.1"
                     /translation="MNVYIIRHAWAEEPNEARWPDDSLRPLTKEGRAKFRRVARRLGR
                     FGVAPRVVVTSPFLRCVETAEILVSSWQEPMVLETVPFLAPQGDIHQLIEWTRTRMGQ
                     LSGDIAWVGHAPDVSKILATLIGGGTFHFAKAGIAAVQFKGPIAAGEGTLLFLIPPSL
                     LL"
     gene            6458..7330
                     /locus_tag="PKI05_00145"
     CDS             6458..7330
                     /locus_tag="PKI05_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOA50631.1"
                     /translation="MIATFEYIEQIVATCRNAARANEETPARRGNLIVLTPEDGSDVI
                     ITGDIHGNRRNFNLIRRVAALDKNPKRHLILQEVCHGGPTYPANAGCMSHTVLEDVAK
                     LKVQYPDRVHFILGNHELSEVTDYPIQKNKQMLNLLFRLGLQHVYGPAADRVRESYLA
                     FIKSCPLGVRLPGDLLVTHSLPEAVDRQGFDVTVFHRPLDMVEFLEHKSVFRLVWGRD
                     YRRENAMAFARLTQAAVLVHGHEPCPQGFATPNDVQLILDCSSDKAAYVMLPLDRQTP
                     WTLDEVVARVQLLA"
     gene            7473..8438
                     /locus_tag="PKI05_00150"
     CDS             7473..8438
                     /locus_tag="PKI05_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOA50632.1"
                     /translation="MMKLTRRGWFAGVGALVGSWLSGSRAATWTEERSSNSSLSVDGF
                     RFVHMTDLHIQPELKAAQGVAACLHHIQSLNPKPDFILTGGDLIMDALQQGEERASML
                     FDLLKKVFRDHNSLPVYHCMGNHDVFGWGKKQGVTEEHPKYGKKMYMEKMELERPYYA
                     FDHKGWRFFVLDSVQPSETELYQGGIDGLQMDWFRQELARKPKEMPAAIVTHIPFITV
                     TVLPDSAKEGRFQIGSASMCAGAQQMAQLLADNNVKLALSGHMHMVDEVSYRGITFAC
                     SGAVSGRWWKGVHKGFPEGYAIIDISPANQISYQYIPFGWQAVES"
     gene            complement(8546..9448)
                     /locus_tag="PKI05_00155"
     CDS             complement(8546..9448)
                     /locus_tag="PKI05_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50633.1"
                     /translation="MASQPIDPKDEDLLEIIQLDRDDPQDAFAQRLLAILRENPALAK
                     RYEEIRRRDAEIGNRLKQGNKLSGLEERILARLGYQNEATDNHLGSSQTLDVAHPESG
                     ALRSSLCEGGASPVVTSRVVTGAPVPRVGRRQWLRWVGALATAGAVGGLGIWVWQRRK
                     EKPRISALALGNAATELFEATLDGFGTGRLLGRENPPQGYRLSRDVQVFRGTVITWRQ
                     VTLADLPAIVFDLSHPGGEKGSLFVIRLVLPDLPPMPPRRPCLRTSRSSAGWWQDNDL
                     ACVLVVLGGPRVYERFLAPPAPLT"
     gene            complement(9483..9992)
                     /locus_tag="PKI05_00160"
     CDS             complement(9483..9992)
                     /locus_tag="PKI05_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOA50634.1"
                     /translation="MDEGSRQPTIEELVVQHAQTVFRYAYRLSGSVADAEDLTQETFL
                     LAAKNIAQLEHPARAQGWLLAITRNAFLRQRRGPHVTLLDDMAATIVESQLDSDDTDE
                     QLIQIALQSLPEQQRVMILMFYFEDMSYKDIARELGVPIGTVMSRLARAKAALRKKLD
                     ALTKKVAPV"
     gene            10195..10404
                     /locus_tag="PKI05_00165"
     CDS             10195..10404
                     /locus_tag="PKI05_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50635.1"
                     /translation="MKKLGSLLIVLALSSLFVGCPKQAPAPAGGAPAPAASSESAPAS
                     DESAQPAEQSQEAPAEQGTGGESQQ"
     gene            complement(10575..>11297)
                     /locus_tag="PKI05_00170"
     CDS             complement(10575..>11297)
                     /locus_tag="PKI05_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOA50636.1"
                     /translation="RLAEDLADYLSKQSIRCKWLHSELDAFERVELLRDLREGAFDVL
                     VGVNLLREGLDLPEVSLVAILDADKEGFLRSETSLIQTIGRCARNVNAKVILYADKMT
                     DSMQKAVEETRRRRKLQEEYNKAHGITPETIKKNIRAGIEAEVAAHNQANALVGRSDE
                     AQYITEEYIAELEAEMLAAAEALEFERAAAIRDRIQLLRKQIGKPVTEAQLKPPQKRR
                     RGGRRNTIHPSPGIDITQFRRK"
BASE COUNT         2396 a         3037 c         3109 g         2755 t
ORIGIN      
        1 cgtgtttctc gagttctttc cgcgcaagtt ggcgatgttt ttcggtcatt aacgcctaaa
       61 ttctggttta acgctcatca ggagcaatga caaagctttg accaattgta tctccaatcc
      121 gtgtcaccca ctcccacagt agtcgccggt gctccttgag aatctgatca tatgcggact
      181 gacccgccag gttttccatt tctcccggat cgttcttcaa atcgataagt tgctcgcgtc
      241 tcgtgccttt ttcataaacc acatacttgt accgggcggt cctgatcatc cgagcggcgt
      301 cactttctac cacgaccgat tctcgccacg tatccacggt gcgaccctcc gccaaaggtc
      361 gaacgctgag gcccagcaaa tgaggaggct tctcgattcc cgcaaagtca cagagtgtgg
      421 gaatcaagtc cagtccaacc gaaacgaggt gtgtccgatc gaccaggccc ggcttcgtta
      481 cccccggcca tgcgacaata aaaggcaccc tcacagcctc ttcgtaaggg atcgacttat
      541 gctccaaacg gtgggcggcg tccaggtcgc catggtcact tgtaaagatg accaccgttt
      601 ttcgatccag tccgtgttgc cggagggcat ctagaacaat cccgatttcg gcatccacgc
      661 gttcggtcaa ccggcaatag gcccagcgat ggagacgcca atcccgttct gtccaatgct
      721 gtcggacata cgcccgaaaa ctttgtgggg gcaggtaact tagggtgacg ccgtcgggct
      781 cgttggatgg aatctcaaaa ttgttgggca acgggggaca cagtcggtcg aagaactcgg
      841 cttcagaaat gccggcagga agggccaagg cctctgccag gcactcgcgg tctcgctgaa
      901 gttccggata tatagcaggt cgtccggttg cctgtgtgaa ggcgtcaatt gccatgagac
      961 aaatgtcatg ggggttgatg aaggatgcta caagcagaaa gggctcagcc ggtggctccg
     1021 aaagaaactc ggcgcacttc ctggccaact cctcccgtgt atccttcgtg agttccccaa
     1081 accccgtgct ttggggcgtc atccctcgca tccagtgcgt cttaccgccg tagaaggtct
     1141 tatagcccgc tcggcggaag atccagccca ttgcctgatc gattgcctcc ttgggggcgg
     1201 ggttctttcc ttcggcgttg gaccgaattt gaaacgcact gggcattcgt ccggttacca
     1261 tactcatccg cgcgggtatg cataccgggt tggcggtgta cgccagctca aatctcgcac
     1321 cttgagcggc caaagaatcc atcgccggcg tgcgaagata gggatttcct gcacagctca
     1381 gcatccccgc gtgttgctga tcggtgataa tgaccaaaag gtttggtctt tcgaccgagg
     1441 gcgtcgatgt tcgcaactcc ggccctggtt cacacgccaa tccttggggc catcccacga
     1501 aaacgatacc cagcgaccaa acagcaagta aaacacctcc ccaacgtgcg aacatgattt
     1561 gcgctcccaa gacaaaaccg ctgtgaccaa agcgcttgcc tttgcctgat gtcaccagtg
     1621 taatattcga gctttactgt gcaacaggac acggcggggt ggcgctccga gcagatttaa
     1681 cctgctacca ggacatcttg ccgaagtcgc ccgttttccg ctaagtttat gcaagttgca
     1741 actcgcattt cagcaattcg ccctggaata gccgcggcca gattcttggg gtagatcgcg
     1801 ttgaacgtgc tgcgcagctc acaagccacg gagggatcaa tgcaccgagt cgcactcctt
     1861 gggcacttta ttgtttacgt gattgtccga ttcctgatct ttgtcgtcca aatgattcca
     1921 cttgccgtgg ggtcttggct tgcgtggtgc ttagcatacc ttttcacata cgttttgcgg
     1981 attcgttatt ccgtggccga cgagaatctc cggtacgctt ttgcggagct ttctccttct
     2041 caacgtcgcg atttgatttt acgaatgtgg catcatcttt ttcttttggt catggaggta
     2101 gcccacacac aagcgaaaat ccgggaagag aactggcgtc aaagggcaac gctcaagaat
     2161 gtggagccgc tggtgaaact tcttttggag gatcgacctg tcattattgt gacggcacat
     2221 tttggcaact ttgaactggg gggctacatg cttggccttt tggggtttcc gagcgcaaca
     2281 atcgcgagaa acctggataa cccttttctc gatcgttttg tgcaacgatt tcgcgctgca
     2341 cggggacaat accttattcc caaggttggt ggggccgacc aggtcgaaga aatgattcaa
     2401 aagggtcaca ccatcgcggt gcttgccgat caagatgcgg gaagaaaagg ctgttttgtt
     2461 cccttctttg ggcgaccggc ctcgaccttc aaggccattg ccctgctttc gctggaatat
     2521 gacgccccaa ttgccgtggt ttacgcgcgg agaaccggta agcctttgca tttcgagatg
     2581 gggctggtgg gcattctcga tcccagagat gtctcagccc ccgacgctgt cacgtttgtg
     2641 acgcagtggt acacatcccg actggaggag atgatccgct ctgccccaga acagtactgg
     2701 tggattcatc gtcgctggaa gacccagccg aagtcaccga agttgcggat tccttcggaa
     2761 caagcggtcc gtagcgccgc gtgactgtgg cataccggta gaagggcatc tcgctgcatc
     2821 ggtcgcctgg tcagatggga tggtattgtc ccgccttgtg aagcgttttc ctaccttgtg
     2881 gcgccactga gattaacctt gtgacgcgat tgagattgag cccaacgtgg cgaagttgct
     2941 cgtctttacc gggctctttt tctccctatt ttcacatact aaaatatcaa tcacatcgct
     3001 tcgggtaact tttgcacctg ctcgtacgcg gtttgttctt acgggatgcg ctagcagtgc
     3061 gggaggccat tcgaatgaca ccaaagcagc gagtcttggc cgcattggaa catcatgagc
     3121 ctgatcggat cccgattgac tttggtggtc atcgctcctc tggtatagcg gctattgctt
     3181 acgcccgttt aaggaaatat ctggggctgc ccgagcgtcc cattcgcgtt tacgatccaa
     3241 ttcagcagtt ggcgatcatt gacgaggatg tcctcaatcg ctttcgagtc gatacgattg
     3301 agctaggtcg ggcctttgcc caggaggatc gttattgggt cgattgggtg ttgccggatg
     3361 gtacaccgtg tcaaatgccc agttgggcgg tgcccgaacg cagtgacggc gggtgggtcc
     3421 tgcggggacc gacgggaaga atcctagggc ggatgcccga tggtgccctg tatttcgagc
     3481 aatgttactg gcccttcgcg gaaaaagacc agccagagcg cgcggcttcc gctttgatcg
     3541 atacgatttg gtgtggaata ccctcgcctc cgggacctat tgtggaaggt ccgggcggat
     3601 tggatcgcct ccgggaaggc gcccgacgcc tacgccaaca aactgatcgt gccatcctgt
     3661 ttctgtttgg gggaaacctg ctggagtgcg gacaatttct ttatcggaat gataactttt
     3721 acatgctttt agccggtgac aggccgcggg cagaaaaggt tctggattcg ctcgtggaag
     3781 cccatctagc caatctcgag cgcgttctcc cggttttgga cggattgatt gatatcatcg
     3841 ttttcgggga tgacttgggg atgcagtccg gaccccagat atcgcccaag atgtatcgag
     3901 agctcttcaa accgcgccat gccgcgatgt ggaaaaaagc caaggagtta gcggacgtca
     3961 aagtgatgct ccattgttgc ggtggtgtcc gtgagttgct ggatgatttg attgatgccg
     4021 ggttagacgc gatcaatccc gttcaaattt cctgcaaagg aatgaacccg acagaattga
     4081 agcgtgattt tggaaagcgg atcaccttct ggggtggagg ttgtgacacc caccgggtct
     4141 tgagttttgg gaccccggag gaagtgaaac agcacgttcg ggaacagatt gagagttttg
     4201 cacccggtgg cgggtttgtc tttcaacagg ttcacaacat tctggcaaat gtgccgcccg
     4261 aaaatattgt ggccatgttc gatgctgttt acgaattcgg ggcgatgtac cgttgattgc
     4321 cggagttatc gtcgggacgc tctgaaggtc gagtttggga gtagcgcagg ccggcagata
     4381 tgcttggccg cttgaacaca cgggcgactg cgaagaatcg tgacgttaaa atagcggttt
     4441 atggcaggct tttgtcacaa gcgattgccg actgtatcga ccaactgatg gagttgcgag
     4501 ggagatgggt tttttgagtc gtttttcttg gcgattactc cggcgtgttt cccccaggtt
     4561 gctcctgaag gccggtcgtt tgtggcttat cccgggattg cggtctttgt cgcggtttcg
     4621 gaaacgagtg gccgcctcag agctttttcc cccgtttttc tttctttccc tcaccgacgc
     4681 ctgtaatctt cgctgtcacg gttgttggat cacaagccag gggcaaccgc gccaagtgag
     4741 tcgtgatgac gtcaatcact tcattgccca agccaaggtt cacggcaacc gttttttcgt
     4801 gttattgggt ggtgagccgt ttctttataa ggacctttgg acaatcctac ggcagaatcg
     4861 ggattgctat tttcaaatca tcaccaacgc gctcctcttg gatgaagcgg ctgtggctac
     4921 ggcaaaggaa gtgggaaacg ccacgatttt ggtaagcctc gacggtttcg aaagtgctaa
     4981 cgacgcgcgt cgcggccaag ggacttttca agctattaag gaagccttcc aacgcctgag
     5041 aaaaggcaaa atactctttg gtgtggctac cacgatcaca tcggagaact atgaagcggc
     5101 cctaagtccc gagtatgtta attgggtcga tgagcagggt gcaatctatc tttggtacta
     5161 catatatcgt cccgtcggcc ccgacccaat gccacaactg gccttaagcc gagccgacat
     5221 cctgaaggtt cgacgacagt tgctaagatt acggcggatt tcgccgatcc tcattgtcga
     5281 tacatattgg gacgccgatg gcaaagcagt gtgccctgca gcattgggat taggctacca
     5341 cataggacca gccggcgata ttgaactgtg tccgcccctt tcctttgcgc gagaaaccat
     5401 tcgtgatcac ggcggtgatg ttttccatac gattaacacg agtgatttcc tccgcggttt
     5461 tcagaaattc gcactttctc ggactcgcgg atgtcccatc ctcgaatgcc ctcatgaact
     5521 ggaggcgttc ttgcgtgaac accatgcccg tgatacaagt ggccgggatg cttttgccga
     5581 actgcgggca tcctctcctc gggcaagtca tcacattccc ggcgaagaac ttcccgagac
     5641 aatgggtacc tatcgttggt tgaaaaaaca cctctttttc ggtttggggg cctacggtta
     5701 gtgtggagga tcgttgcgat cgtaaagggc catattctct aaggacattt cttgaggttt
     5761 tcgcgagccc gtatctcaaa aaacgcactt aatgggttga aagcatgaat gtgtacatca
     5821 tacgacatgc ctgggcggaa gaacctaacg aggcccgctg gccggatgat agtctccgcc
     5881 ccttaacgaa agaaggtcgg gcgaagtttc ggcgggtggc acggcggctt ggtcggtttg
     5941 gggttgcccc gcgcgtcgta gtcaccagtc ctttcctgcg gtgtgtggag acggcggaaa
     6001 tcttggtgag cagttggcaa gaacctatgg ttctggaaac ggtaccgttc cttgcaccgc
     6061 agggtgatat tcaccaattg attgaatgga cacgtacgag gatgggtcag ctttccggcg
     6121 acatcgcatg ggttggacat gccccagacg tttccaagat tctagccaca ctcattggcg
     6181 ggggcacttt ccatttcgca aaggctggaa ttgccgcggt gcaattcaaa ggaccaattg
     6241 ccgcgggcga ggggacgcta ctttttctca tacccccgtc gctgctgcta taaagagtct
     6301 tctttcatgt tgtgctgtga agatggggcc cttttttggg tgcgcacatg ggcctttctt
     6361 cgcaccggcg tgaccaagat aataacggat atcgcgcaga tatgacgata aggtggccaa
     6421 ttctttggtg ctatgatcag ccttgaggct tatcaggatg attgccacat tcgaatacat
     6481 tgagcaaatc gtcgcaacgt gccggaatgc cgcccgggcg aacgaagaga cgcccgcccg
     6541 tcgaggaaat ctcattgttt tgaccccgga agacggttcc gatgtaataa ttacagggga
     6601 catccatggg aatcgccgga attttaacct gatccggcga gtggcggctt tggacaagaa
     6661 cccgaaaagg cacctcatct tgcaggaagt ctgtcacggc gggccgacgt atcccgccaa
     6721 tgcggggtgt atgtctcata ccgtgctcga agacgtggcc aaattgaagg tacagtaccc
     6781 tgaccgcgtc catttcattc tgggtaacca tgaattgtca gaggtgacgg attatccaat
     6841 tcaaaagaac aagcagatgt tgaatctgct cttccggctg gggctgcaac atgtttacgg
     6901 gccggctgcc gaccgcgtcc gcgaatcgta tttggccttc atcaagtcct gccctcttgg
     6961 ggtccgctta cctggtgatc ttctggtaac ccacagtctt cccgaagcgg tcgatcgaca
     7021 aggctttgac gtcacagtat ttcatcgtcc gctcgacatg gtcgagtttc tcgagcacaa
     7081 gagcgttttc cgcctggttt ggggacgcga ttaccgtcgc gaaaacgcaa tggcctttgc
     7141 ccgccttacc caagcggcgg tactggttca cggtcatgaa ccatgtccac aaggttttgc
     7201 gactccaaac gatgtccagc tcattttgga ctgctcctct gacaaggcgg cctatgttat
     7261 gctgccgttg gaccgtcaaa caccgtggac cttggacgag gtggttgccc gcgtccaatt
     7321 gttggcttaa gcgaccacat tgggctgacg atctttttgt caaaaaacgg ttaacgcgac
     7381 gagcgaagcc tttccctccg cctggcatcg gatagaatct cgataagcct gtgttgtcct
     7441 ttcccacctt ttccaatatt gagaggagac cgatgatgaa attgacacgt cgcggttggt
     7501 ttgcgggcgt cggcgcgctg gttggtagtt ggttatcggg atcacgcgct gccacttgga
     7561 cggaagagcg ttcctctaat tcctcccttt cagtcgatgg ctttcgcttt gtccacatga
     7621 cagaccttca cattcagccg gagttaaagg ccgcgcaagg ggtcgctgcc tgcctgcatc
     7681 atattcaatc cctaaatcca aaacctgact tcatcctcac gggtggcgat ttgatcatgg
     7741 atgcacttca acaaggggag gaacgcgcgt cgatgctgtt tgatctgctg aagaaagtct
     7801 tccgcgatca caatagcctc cctgtgtacc attgcatggg caatcatgat gtctttggct
     7861 gggggaaaaa gcagggtgtt accgaggagc atcccaaata cggcaagaag atgtacatgg
     7921 aaaagatgga gctggaacgc ccttactacg cctttgacca caagggttgg cggttcttcg
     7981 tcctggatag tgtccaacct tcggaaacgg aattgtatca gggaggaatc gacggccttc
     8041 aaatggattg gtttcgccag gagctggccc gcaaacccaa ggaaatgccg gctgccatcg
     8101 tgacccatat cccgtttatc acggtgacgg tgctccccga cagcgctaaa gaaggacgat
     8161 ttcagatcgg ttctgcgtcc atgtgtgcag gtgcccagca aatggcccaa ttgcttgccg
     8221 acaacaacgt caagctagca ttatcgggtc atatgcatat ggtggatgag gtatcgtatc
     8281 gagggatcac attcgcttgt tcgggagccg tctcgggccg gtggtggaaa ggcgttcaca
     8341 agggctttcc cgagggctac gcgattatcg acatcagtcc ggccaatcag atttcctacc
     8401 agtacatacc ctttgggtgg caagccgtag aatcctgacc tcgcggtttt gtgcccgagg
     8461 gacgcttgca aacgtatctg atgcccaact tcattgtctt aagaggctgg caacccgggc
     8521 ccagaccgcg cgaaggcctg gatccttagg ttaagggtgc gggtggggca aggaaccgct
     8581 cgtacacgcg gggtcctccc agtacaacca gaacgcaagc cagatcattg tcttgccacc
     8641 aaccggctga agagcgactt gttcgcaaac aaggccgtcg cggtggcatg ggcggaagat
     8701 caggaaggac cagccggatc acaaacagcg aacctttttc gccccccgga tgactgagat
     8761 caaaaacgat ggcgggcaaa tccgcgagtg tgacttgccg ccaggtaatc accgttccgc
     8821 gaaatacctg cacgtcgcga ctcaatcggt aaccctgggg cgggttttcc cgtccaagca
     8881 agcgaccggt gccaaaaccg tccaatgtgg cttcgaacaa ttcggtggct gcatttccaa
     8941 gggcgagtgc cgagatacga ggtttttctt ttcggcgctg ccagacccaa attcccagtc
     9001 ctcccactgc gcccgcggtt gccaaagccc ccacccaacg taaccattga cgacggccaa
     9061 cgcgaggcac tggtgctccc gtgactacac gagacgtgac tactggagaa gctcccccct
     9121 cgcacagact cgaacgcaac gccccagact cagggtgtgc cacatcgagc gtctgagacg
     9181 atcctaggtg attatcggtc gcttcgttct gatatcccaa ccgggctaaa attcgttctt
     9241 ctaatcctga cagcttgttc ccctgtttga gccgatttcc tatctcggca tcgcgtcggc
     9301 ggatttcctc gtagcgcttg gcgagggcgg gattttcccg caagatggcc aagagccgct
     9361 gcgcgaacgc atcctgcgga tcatctcgat ccaattggat gatctctagg aggtcctcgt
     9421 ctttagggtc aatgggttgg gaggccatta ttgttagtca tcatttttta aatcctggga
     9481 tttcaaacag gggccacctt tttggtgagc gcatcgagct tctttcgcag agctgccttt
     9541 gcccgcgcca gccgactcat gaccgtaccg attgggacgc ccaattctcg cgcgatgtcc
     9601 ttatacgaca tatcttcgaa gtaaaacatg aggatcatga cgcgctgctg ttcgggcagc
     9661 gattggaggg cgatttggat caattgttcg tcggtatcat ccgaatccag ttgcgattca
     9721 acgatggttg ccgccatgtc atccaaaagc gtaacatggg gccctcttcg ctgcctgagg
     9781 aaggcatttc gtgtgatggc cagcaaccac ccctgggcac gcgccggatg ctcaagctgg
     9841 gcaatattct tggctgcgag taaaaatgtc tcctgggtca aatcttctgc atcggcaacg
     9901 gaccccgaca gacggtacgc atagcgaaaa accgtctgag cgtgctggac caccaattcc
     9961 tcaatcgtgg gctgccgcga gccttcatcc atctttgact acccattaag atgcgcaacc
    10021 accggccatt attccccaga tgcttacctt tggcatattc gcagccgggt atcttccatt
    10081 aaatgtaacg ttcgcgggaa taaaacacca acggagcgca tcgtactttt ttgtacgcga
    10141 cctccggcgg tcgggggtgc ctttaacggt tttttgggag aaagggtatc tcggatgaag
    10201 aagttgggat ccttgttgat cgtcttggct ttgagttcat tgttcgttgg ctgccccaag
    10261 caggcgccgg caccggccgg tggtgcccca gctccagccg catcttctga gtcggctccc
    10321 gcatcggatg aatccgccca gccggccgag caatcccagg aggcaccggc tgagcaaggc
    10381 accggcggtg aaagccagca gtaacctctt ggcttcaaag tgattatcat tttgaaactg
    10441 gccgtcagtt aggccgacgt atcgagcgct atgtccggaa ggcccttcgg cggggctaat
    10501 ccccgtcgaa gggcctccat ttattgcaaa gggcaacgtt gttgaccccg ctctttggat
    10561 agcccgggtt tgttctattt tcgccggaac tgtgtgatat caattccagg ggaagggtgg
    10621 atggtgtttc tgcgtccacc gcgacggcgc ttttgtggcg gttttaattg cgcctctgtg
    10681 acgggcttgc cgatctgctt tcgcaaaagc tggattcggt cgcggatagc cgcggcccgc
    10741 tcaaattcga gggcctcagc ggcggcgagc atttcggcct cgagctcggc gatatactct
    10801 tcggtgatgt actgagcctc gtcagaccgc cctacgaggg cgttggcttg gttgtgggcc
    10861 gcaacctcag cctcgatgcc agcgcggatg ttcttcttga tcgtttcagg agtgatcccg
    10921 tgcgccttgt tatattcctc ctgcaatttt cgccgtcgcc tcgtttcttc caccgctttc
    10981 tgcatggaat ccgtcatttt gtcagcgtag aggatgacct tggcattaac atttcgagca
    11041 caacggccaa tggtctgaat caacgaggtc tcactccgca agaatccttc cttatcggca
    11101 tcgaggattg ccaccaggga gacctctggc aggtccaatc cttcccgcaa gaggttaacc
    11161 cccaccaaaa catcaaaggc cccctctcgc aaatcccgta gaagttcaac ccgttcaaag
    11221 gcgtccaatt cactgtgaag ccatttgcac cgaatactct gcttgctcaa ataatccgca
    11281 aggtcctcgg ccaggcg
//
LOCUS       DAOPTR010000005        11064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_4964_length_11064_cov_3.644109, whole genome shotgun sequence.
ACCESSION   DAOPTR010000005 DAOPTR010000000
VERSION     DAOPTR010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 11064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11064
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4964_length_11064_cov_3.644109"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..1254
                     /gene="hemB"
                     /locus_tag="PKI05_00175"
     CDS             205..1254
                     /gene="hemB"
                     /locus_tag="PKI05_00175"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOA50637.1"
                     /translation="MGHSRREELWVGQCRMPGFPLTRLRRLRYHPAIRRMIQATHLDP
                     RRWILPLFIHAKGDEKKPIKSMPGHFQLPLSGLRAEIKEIMQLGLGGVLLFGIPAHKD
                     AMGCDALRDSGIICQAIKAIKDVAPDLLVITDECFCEYTDHGHCGVLGRAGNQDTVDN
                     DKTLRMLAWQAVAHARAGADMVAPSGMMDGMVQAIRSALDEAGFTHLPIMSYSAKYSS
                     AFYGPFREAAESAPRFGDRRSYQMDPAAAAEQALREVELDVAEGADIIMVKPALAYLD
                     VIRMVKDNFPGLPLAAYNVSGEYSMVQAAAEKGWLDPRRIALEMLTAIHRAGADIVIT
                     YWAKDAAHWLDSSDL"
     gene            complement(1280..2035)
                     /locus_tag="PKI05_00180"
     CDS             complement(1280..2035)
                     /locus_tag="PKI05_00180"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOA50638.1"
                     /translation="MTEIREPRIVAAAEREMQKWRTAQQIADRIIRFDTAHGAQQKKI
                     GPYITISRQAGAGAEQIAHAVGERLGWEVVDKNVLDAIAKHFCLERSQLENLDESSGN
                     WLRDTLFLWLDPHIISADKFLACLEKVVWMAARKGNVVFVGRGVRFLLPDEHGLAVRL
                     VASEKYRVERIRREKGLSEAAARRFVHELEQTRRQFVQRFFHKDVDDPQWYDLVINVE
                     RWGVEETANLIVTAWRAREEQMKTVAKPAEAVV"
     gene            complement(2411..3289)
                     /locus_tag="PKI05_00185"
     CDS             complement(2411..3289)
                     /locus_tag="PKI05_00185"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOA50639.1"
                     /translation="MAGISRRGFLAGLGAVGGVALSGKWAIADPHKPTLKSGVDMITL
                     GRTGLKTSVLGVGTGTVGGSQQLAMGSEGFGKMLRYALERGVHYIDTADNYNTHIFIR
                     LALQATPRVPREKYFIQTKTRARTAEVLHADIERFRRELRVDYIDSVLMHCMTTGDWP
                     TVMRPVMDALYDEKQKGRVKAVGVSCHGWEPLVASVDVDWCDVQLVRINPFGAKMDGE
                     PKDVAAVIKKMHDAGRGILGMKIYGETGFNSREERLQSLKYVLGLGCVDCFTIGFTSI
                     EQIDETLQLIEEALKA"
     gene            complement(3565..3939)
                     /gene="rplT"
                     /locus_tag="PKI05_00190"
     CDS             complement(3565..3939)
                     /gene="rplT"
                     /locus_tag="PKI05_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOA50640.1"
                     /translation="MRTKTSATRRRRVKRLFRRAKGFVGGRRRLLRTVKETLVRAGVY
                     AYRDRRARKRVFRRLWITRISAAVRQRGMLYSRFIDGLNKAGIKLDRKALAEMAISDP
                     EGFDQVVEKAKEALQAAQSPLW"
     gene            complement(4108..4302)
                     /gene="rpmI"
                     /locus_tag="PKI05_00195"
     CDS             complement(4108..4302)
                     /gene="rpmI"
                     /locus_tag="PKI05_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012095718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOA50641.1"
                     /translation="MPKQKTKKSAAKRFKITGTGKVKHRSAGTSHLATRKSKKRRRQL
                     RGTDVLSPVEAKRVIPLIKT"
     gene            complement(4529..5095)
                     /gene="infC"
                     /locus_tag="PKI05_00200"
     CDS             complement(4529..5095)
                     /gene="infC"
                     /locus_tag="PKI05_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOA50642.1"
                     /translation="MEWRVGLFVGRFQKERSLAIEEKNYRVNERIRSPSVRLIGPEGQ
                     QLGVVSREQALAAAREAGLDLVEVAPNENPPVCRILDFGKFKYLQKKKQQKSQAAQIK
                     VKEIRVRPGTGEADLNVKINRAREFLTKKDKVLVSVMFRGREAAHMEEGEKVLNLIIE
                     RLADVGKVEGAPSRQPKRIVCTIVPAVK"
     gene            complement(5271..7244)
                     /gene="thrS"
                     /locus_tag="PKI05_00205"
     CDS             complement(5271..7244)
                     /gene="thrS"
                     /locus_tag="PKI05_00205"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOA50643.1"
                     /translation="MLRVIFPDGSAKEYRERVRPIDIAAELGPRLAKSAVAAMVNGSV
                     VDMTAPLPQEGEVQLRILTERDHEALNVLRHSAAHVMARAVLRLFPKAKLAFGPPVEN
                     GFYYDIELERSLTDDDLPRIEEEMAKIIAQDEPFERIEVPWERAVEICRDLDQPFKVE
                     HLQEGLASERVVSFYRQGEFLDLCEGPHVPSAGWIKAFKVLSVAGAYWRGDASRQQLQ
                     RLYGTAWFSQKDLDEYLRRLEEAKKRDHRVLGKRLELFTIEPMVGSGLILWLPKGAII
                     RRQLEDFLFGELIRWGYQAVYTPHIGRVELYQTSGHYPYYADSQFPPIQMADGERYLL
                     KPMNCPHHIMIYKSRPRSYRELPLRLAEFGTVYRFEQSGELTGMTRVRGFTQDDAHIF
                     CTEEQVADEFRGCIQMTQFVLKTLGFTDYRVRLSLRDPKSNKYVGDPEVWERAESALK
                     QVCQEMDLPNLETAIGEAAFYGPKADFIVSDCIGRQWQLGTVQLDYNLPSPQRFNLEY
                     IGPDNRPHQPVMIHRAPFGSLERFTGILIEHFAGAFPLWLAPEQIRVLTVSEKFDEYA
                     RRVEGRLREAGLRVSGDYRAEKVGAKIRDAQLEMIPYMAIVGAREQEQGTVALRDRLK
                     GDLGAMPLDEVISRLQEEIRLKLIRRSDERKNG"
     gene            7821..8789
                     /locus_tag="PKI05_00210"
     CDS             7821..8789
                     /locus_tag="PKI05_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50644.1"
                     /translation="MAPSQSLFYYDEGLKLLPINLAIDVRRPHAQAFVSHAHADHIAR
                     HGYTLCTAETAAFLGLRLGPRPTREMSYGKNIDWGGVSLTVYPAGHCLGSAMLLVEHQ
                     GQRLLYTGDFKLRPALTTPAAQPVKADILIMETTYGEPRYRLPPEDEVIEDLACCIRD
                     TWDRGRCPVIMAYALGKAQEVSRRLSEAGIGVVQHSSVATISRIYEQFGVSVGNYRPY
                     DGRLERGEVLITSPQQRWDCPTESVVRIAVTGWALDPSAKYRLGADVVFPLSDHADYE
                     ELFQMVQTVQPQLVYCTHGPATFVRDLMERGWGARLLGRPTQKLLF"
     gene            9112..9303
                     /locus_tag="PKI05_00215"
     CDS             9112..9303
                     /locus_tag="PKI05_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50645.1"
                     /translation="MSICERKRELRRKRHRREKYRIFKRKLLTANASERAIIAEKIRK
                     LAPDPEVVLKNLGLLESQR"
     gene            complement(9394..>11064)
                     /locus_tag="PKI05_00220"
     CDS             complement(9394..>11064)
                     /locus_tag="PKI05_00220"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOA50646.1"
                     /translation="TESEEFRVRPGECFVIGKTSFSLSRQRVSVAADHPTPTQQKAFG
                     PQYLRQVVFRNPNYRLEVLLRLPRVVSRAAADAELAARLVNLLLAGMPNVEAVAIVAL
                     DKPYPATSCSSDDGGAQEVAPLDVDIRVIHWDRRQLTEGDFCPSRQLILDAWRRRQSV
                     LHMWRELVPEGTSKTVSPGQTTHSQPSHRRAGPTYTARQNIDWAIGTPIVAEGASPWV
                     IYVAGRFHSEWGSGATTPDTLQEDAKFLELVAEMTGALLQLRRLEHRQAILSQFLPRP
                     VLDRLGPEDWEAALQPREVVATVLFCDLRGFAHETERQADQLLPLLDRISAALEVMTR
                     RILEHGGVIGDFQGDAAMGFWGWPLPREDRVLAACRAALAIRHDFSQAAQTPGHPLAG
                     FHIGIGLASGRVVAGKIGTPDHAKITVFGPPVNLAARLEGITKLIHVPILMDDETARA
                     ARTLVTPEIGRIRPIARIRPYGLDQAVLVHELLPPECEYPLLKEHHLHLCEQAVEAFI
                     RGDWERAFSLFHQLPAEDRGPDFVTAYIAQHHRVPPNSWDGTIVMSSK"
BASE COUNT         2426 a         3325 c         2905 g         2408 t
ORIGIN      
        1 tcaatcttgc tcgctggatt gtggaacgaa acgttcccaa cgcatggatc gtggattttg
       61 ccaatgccgc gccagcttca tagcgttagc gaccatagtg gcgcacgttg gtgttacgat
      121 ttagggaatg cgtgaagaat tgctacaacg ttgggcgggg tattcgctcg gagatttgat
      181 taacgtctga atgaggagat caatatgggt cattcacggc gtgaagagct ttgggtagga
      241 cagtgccgga tgccaggctt ccccctcaca cgcttacgta ggttgcgtta tcatccggcc
      301 attagacgga tgatccaggc aacccacctg gacccacggc gctggatcct gcctttattt
      361 attcacgcta agggcgacga aaagaaaccc atcaaatcga tgcccggaca ttttcagtta
      421 ccgttgtcgg gcctccgcgc ggagatcaaa gagattatgc aattgggatt aggtggggtc
      481 ctccttttcg gcatccctgc gcacaaagac gcaatggggt gcgacgccct gcgtgactct
      541 gggatcatct gccaggcgat caaggccatc aaagatgtgg ccccagatct gcttgtcatc
      601 accgacgagt gcttttgcga atacaccgac catggacact gtggcgtctt gggtcgggcc
      661 ggaaaccagg acacggtgga caacgataag acgttacgga tgttggcgtg gcaagcggtg
      721 gcccatgccc gcgcaggtgc cgacatggtg gcccccagtg ggatgatgga cggaatggtt
      781 caggctattc gtagtgccct cgacgaggca ggctttacgc atcttcccat tatgagctat
      841 agcgccaagt attcgagcgc gttttatggt cccttccggg aggccgccga gagtgcccca
      901 agatttggcg atcgccgaag ttatcagatg gacccagccg cggccgccga gcaagccctc
      961 cgtgaggtcg agctcgatgt tgccgagggg gctgacatca tcatggtcaa gcctgccttg
     1021 gcctatctcg atgtcattcg gatggtcaag gacaattttc ccggtctgcc cctggctgcc
     1081 tataacgtct ccggtgagta cagtatggtc caagccgccg cggagaaagg ctggcttgac
     1141 ccgagaagaa tcgccttaga gatgctcacc gccatccacc gagccggagc ggatatcgtc
     1201 atcacctact gggccaaaga cgcggcccac tggctagact catccgacct ataggtcggc
     1261 agcctcgaga gactcacgct tacaccaccg cctccgcagg cttggcgact gtcttcatct
     1321 gttcctcccg cgcccgccaa gccgtgacaa tcaaattggc cgtttcttcc acgccccacc
     1381 gctccacatt gatgacaagg tcgtaccact gagggtcatc cacgtccttg tggaagaatc
     1441 gctggacgaa ctgacgccgc gtctgctcta attcgtggac aaagcgacgg gcggcagctt
     1501 cgctcaatcc tttttcccga cggatacgtt ccactcggta cttctccgaa gccaccagtc
     1561 gcaccgccag tccatgttca tcggggagga gaaaacgaac acctcggcca acgaacacga
     1621 cattcccctt gcgcgcagcc atccagacga ccttctccaa gcaggccagg aacttgtcag
     1681 ccgaaatgat gtgtggatct agccacagaa ataatgtgtc gcgaagccaa ttcccggaag
     1741 actcgtccaa gttctcaagc tgactgcgtt ccaaacaaaa gtgcttggca atggcatcaa
     1801 ggacgttctt gtccacgacc tcccaaccca acctttcgcc aacggcatgg gcgatttgct
     1861 cggccccggc tccagcctgg cgggaaatgg tgatatacgg accaatcttc ttctgctgag
     1921 caccgtgagc tgtatcaaag cgaatgatgc gatccgcaat ctgctgggcc gtcctccatt
     1981 tctgcatttc ccgctccgcc gccgccacaa tcctcggttc gcggatctct gtcatagggc
     2041 cctcccatca ttaacgagaa gctcagaaaa ggaaaagtca catcggcctt gcaccttggc
     2101 cctctgtggt cagatcactg ccgatccctc aatatctgtc cacctagatt atacccaaat
     2161 tggaggtaac gaccaataaa aaaacgtgac cctttctttg cgcagactcg cgtcccggcg
     2221 tcttcacgag acgaccaacc atctgccggg tcaagaaaca acccacttgg gggatacggc
     2281 tatttgcgaa cgccgtccca atttgtccga aactgcgtca agctgctttt accgtatgcc
     2341 atcaagaaag gcgcagagag acccaggggc tccggagccg gcggcgatac ttcccaggag
     2401 accggttccg tcaggcttta agggcttcct caataagttg cagggtctcg tcgatttgtt
     2461 cgatgctggt gaacccgatg gtaaagcagt ccacacagcc caagccgagg acgtatttca
     2521 gcgattgcag gcgttcctca cgagagttga aaccggtttc gccataaatc ttcatgccaa
     2581 ggatgccgcg acccgcatcg tgcattttct tgatgacggc tgcgacatct ttcggctcgc
     2641 cgtccatctt cgctccgaag gggttaatcc ggaccaattg aacatcgcac caatcgacat
     2701 cgaccgaggc cactaggggt tcccagccat ggcacgaaac accgacggct ttgacccgcc
     2761 ccttttgttt ttcgtcgtaa agggcgtcca tgactggtcg cattaccgtg ggccaatctc
     2821 ctgtggtcat acagtgcatg agcacagaat caatgtaatc cacccgcagt tcacggcgaa
     2881 atcgctcgat gtcggcgtgg aggacttcgg ccgttcgagc ccgcgtcttc gtttggatga
     2941 agtatttctc gcggggcacc cgcggggttg cctgcaaggc caaacggatg aaaatatgcg
     3001 tgttgtaatt gtctgcggtg tcaatgtaat ggactccgcg ttccagggca taccgcaaca
     3061 tcttgccgaa gccttcactg cccatcgcca attgctggct gcccccgacc gtccctgtgc
     3121 ccactccaag gacactcgtc ttcagccccg tccggcccag cgtaatcata tcgacgccac
     3181 ttttcagggt aggcttatgc ggatcagcaa tagcccattt ccccgaaaga gctactccac
     3241 ccacagcccc taaacccgcc aaaaacccgc gacgcgaaat gcctgccata ggaagtcctc
     3301 ctattcgttt ccctattcac acccaatcaa ctacgtgtaa cccatgaacg ttgacgcggc
     3361 cttttctagg tctttcggaa cacggcacac taaaacactg ccttattttg gccaccgcga
     3421 tgccccattt tatacgagaa gtttgccaaa ttccaccaac tgtggctgac attttacaag
     3481 gacagtcact ccgtctggca ataaaaaacg cccgagatat tcgggcgttc catatggtca
     3541 ccgttttgcc gagtgcgaat caaattacca caggggggat tgggcagcct ggagggcctc
     3601 tttggccttt tcaaccactt ggtcaaaacc ctctggatcc gaaatggcca tttcggccag
     3661 ggcctttcgg tccagcttaa taccagcctt attcaaacca tcgataaatc ggctatagag
     3721 catcccccgc tggcggaccg ccgcgctgat gcgtgtgatc caaaggcggc ggaatacccg
     3781 cttgcgtgca cgccggtctc gataggcgta aaccccggcc cgaaccagcg tttctttcac
     3841 cgttcgcagg agccgccgac gtccgccgac aaacccttta gctcgacgga ataagcgttt
     3901 aactcgacga cgacgtgttg cactcgtttt agtacgcatg atcaatcatc tcctcaccac
     3961 ccgggatcaa ggcctcctcg gccgcagccg aagacgtttc ctgcccgtcc cgtgaatctc
     4021 attctttaag agcctttaac gtttcccttg atcaatcgct cactacattt gaaaagctgg
     4081 cggcaagacc gaaactttgt gcgatcctca ggtcttgatc aaaggaatca cccgcttggc
     4141 ttccaccgga gaaagcacat cggtgccgcg caactggcga cgccgcttct ttgattttcg
     4201 cgtagccaaa tggcttgtgc cggcactgcg atgcttaact ttgcccgtgc ctgtgatctt
     4261 gaacctcttg gcggcggatt ttttcgtttt ttgctttggc attttgacag tccaactacg
     4321 atcgtgatga cctgctacgc tttcccgtga cgaaaaaacc ccggctcccc tctccctcaa
     4381 agctggcgag cgtcgaaaac atgaattcta cccgtttcac cggaaccgcg gaaggccctc
     4441 ggcgaaaagg ctttctccgg cgaggctcaa acacctcgcc ccggcggcat actgcccggc
     4501 gtaacccggt ctccagcagt acgcacggtc acttaacggc ggggacaatc gtgcagacga
     4561 tgcgtttcgg ttgacggctg ggagccccct cgacctttcc cacgtcggcc agacgctcaa
     4621 tgatcagatt gaggactttc tcaccctctt ccatgtgggc ggcttcccgt ccccgaaaca
     4681 tcacggacac caagactttg tccttcttgg tcaggaactc ccgggcacgg ttgatcttca
     4741 cattcagatc ggcttcccct gtgccaggtc ggacacggat ctccttgacc ttgatctgcg
     4801 ccgcttggga tttctgctgc tttttctttt gcaggtactt gaacttgccg aaatccaaaa
     4861 tccggcaaac ggggggattc tcgttggggg caacttctac aagatcaagg ccggcttctc
     4921 gggccgctgc caatgcttgc tcgcggctta ccactcccaa ctgctgacct tctggaccaa
     4981 tcaaccgcac cgatggactg cggatccgct cattgacgcg ataattcttc tcctcgattg
     5041 ctagactcct ctctttctga aaacgaccaa caaacaaacc tactcgccat tccaatgaac
     5101 actcggccag cctcttccag gggcccttaa tcttttcgtt agagtatgac cagagaaaaa
     5161 ggcaaagtca acacctttgg gtgatatttg ccatgagtat ctcctgcctt ctcgtggatg
     5221 tctcctgtcc ccaatcaggt tcactctccc gtgtccagca gaaaacggtc tcatccattc
     5281 ttcctctcgt cagatcgacg aataagcttc aaccgaatct cttcctgaag tcggctgata
     5341 acctcatcca aaggcatggc ccccaggtcc cccttcagcc gatcgcggag ggccaccgtt
     5401 ccctgctcct gctcccgcgc ccctacgatg gccatgtagg gaatcatttc gagctgggca
     5461 tcgcgaattt tcgccccgac cttttcggca cggtagtccc ccgaaactcg taaccctgct
     5521 tcgcggagtc ggccttccac acgccgcgcg tactcatcga acttttcgct cacagtcagc
     5581 acgcggattt gctcgggggc caaccaaagc ggaaacgccc cggcgaaatg ctcgatcaag
     5641 atccctgtga aacgttccag cgaaccaaag ggggcacggt ggatcatcac cggctgatga
     5701 ggtcggttat cgggaccgat atattccagg ttaaaccgct gcggactggg caggttatag
     5761 tcgagctgga cagtccccag ttgccactgc cgtccgatgc aatcggagac aatgaaatcg
     5821 gccttgggcc cataaaaggc tgcctctccg atggctgttt ccaaattggg caggtccatt
     5881 tcctgacaga cctgcttcag tgcggactcg gcccgttccc aaacctccgg gtcgccgacg
     5941 tacttgttgc tcttgggatc acgcaggctc agcctgacgc ggtaatccgt gaagcccaac
     6001 gtcttcaaaa cgaactgagt catctggatg caaccgcgga actcgtcagc gacttgctcc
     6061 tccgtgcaaa agatatgggc atcatcctgg gtaaagccgc gaacgcgggt catgcccgta
     6121 agttctcccg attgttcaaa ccgatagacc gttccaaatt cggcaagccg gagcggcagt
     6181 tcccgataac tcctcggtcg agacttgtaa atcatgatgt gatgcggaca attcatcggc
     6241 ttcagaagat accgctcccc atcggccatt tggatgggtg gaaactgact gtcggcatag
     6301 taggggtagt gacccgaggt ttgataaagc tccacccggc caatgtgcgg cgtgtaaact
     6361 gcctgataac cccagcggat caattcccca aacagaaaat cctccaattg gcgacggata
     6421 atcgcccctt ttggcagcca aaggatcaag ccggagccca ccatcggttc gatggtgaag
     6481 agctccaacc gcttgcccaa aacacggtga tcgcgttttt ttgcctcttc caaacggcgg
     6541 aggtactcat cgaggtcctt ttgactgaac caagctgtcc cgtacagccg ctgcaactgc
     6601 tgacgcgagg catcaccccg ccagtacgcc cctgcgaccg aaagcacttt gaaggcctta
     6661 atccagccgg ccgagggcac atgggggcct tcgcaaaggt ccagaaactc cccctgacgg
     6721 taaaacgaga ccaccctctc cgaggctagc ccctcctgaa gatgttcaac tttgaagggt
     6781 tggtccaaat cccggcaaat ttcgaccgcc ctttcccaag gcacctcgat ccgttcaaag
     6841 ggctcatcct gggcgatgat tttggccatc tcctcttcga ttcgggggag atcatcatcc
     6901 gtgagacttc gttctagctc gatgtcgtag tagaaaccat tttccaccgg tggaccaaat
     6961 gccaatttgg ccttggggaa aaggcggagg accgcgcgtg ccatgacgtg ggctgccgag
     7021 tgtctgagga cgttcagcgc ctcatggtcc cgctccgtga gaatccgtaa ctgcacctcc
     7081 ccctcttgcg gcaatggggc cgtcatatca accaccgaac cattcaccat cgctgcgacg
     7141 gcactttttg ccaaccgtgg acccagttcg gcggcaatgt cgatgggccg aacacgttcc
     7201 cgatattcct tggcgcttcc gtcaggaaag atcacccgga gcattttggt atattctcca
     7261 aacaacctat tttgacactg ggaaagattg aggaagccac gccggtgcgt ggtataaacc
     7321 tatctagtat cgtcttggcg agcgaatatt aaaagggcaa cctgggcagg ttatgcgcac
     7381 ctgaagccca ccgtaagctc gaagtgtctt ttcactggca gtgcgtttgt cacagtttgt
     7441 aggggcaggc ccctgtgcct gcccgatctg atggaaagcg aggcccactc gtgggcgttt
     7501 aaaccatcca tacagcgata tccacgccgg agccctctcg cccggccggt tggcgattgg
     7561 ggctggtatt atcgtggccg cgacggcgca agtcatttaa caagcgggtc accccaatgg
     7621 ctggacctac caacacatct caacacgtca cctcctggcc aaaggtcacg ctgttgtgat
     7681 cgggtcaaat ccgttacggc aaacggccaa gtcaggacct tccttctggt ggaaggagtt
     7741 gggacaatca tcggtaagcg tcccatggct agttcttggg cccggggaga caacaaacaa
     7801 ttttcacgag aaacgtcatc gtggcaccgt cccaatccct tttctattac gatgaaggcc
     7861 taaagctatt acccatcaac ttggccattg atgtccgacg gccccatgcc caggcctttg
     7921 tttctcatgc tcacgccgac cacattgcgc gacacggata cacattatgc acggcagaga
     7981 cggcggcctt cctcggcctg cgccttggtc cacggccgac gcgggaaatg tcctacggaa
     8041 aaaacatcga ctggggtggc gtctctctga ctgtttatcc cgcgggccat tgtttggggt
     8101 cggccatgtt gcttgttgag caccaaggac aacggcttct ttacactggc gattttaaac
     8161 tacgccccgc tttgaccaca ccggctgccc aacccgtcaa ggcagacatc ctcatcatgg
     8221 aaacaaccta cggggaaccg cggtaccgcc tgcctccgga agatgaggtg atcgaggatt
     8281 tggcatgctg catccgcgac acgtgggacc gaggtcggtg ccctgtcatc atggcttatg
     8341 ccctgggtaa ggcccaagag gtgtctaggc gtttgagtga ggccgggata ggtgttgtcc
     8401 aacactcgtc cgtggcaacg atcagccgga tttatgagca attcggagtc tcggtaggaa
     8461 attaccgtcc ctacgacgga cgcttagagc gcggtgaggt gctgatcaca tcaccccaac
     8521 aacgttggga ctgcccaacg gagtcagtcg ttcggattgc cgtcacaggt tgggccttgg
     8581 acccttcggc caagtaccgt cttggggccg atgttgtgtt tcccctctct gatcatgccg
     8641 actacgaaga actcttccag atggtgcaaa ccgttcagcc ccaacttgtt tattgcacgc
     8701 acgggcccgc gacattcgtg cgggatttga tggaacgtgg ctggggcgcc cgccttttgg
     8761 gtcgccccac acaaaagctc ctgttttgat agcccgcctc gctgtgcttc accgtcttca
     8821 gtttggcggc tcatctgccg cctcgttacc gaaacgaaag caaccataac gcgaggagcc
     8881 agcggaagac cacaggccaa cgcccacaaa aaaaggcgat gacggtcgcc ccacattttc
     8941 cctgtccccc ctgccgttaa aatcgaacgc caaccgggtc tattgacgtc ctgcgaagcc
     9001 acgagtaaaa ggaacgtgat ggggtggtgg tagtctggga aaaagctccc atgaggagcg
     9061 cgccagattg gcacactatg aatcattagt ttttctcaaa ggaggttgat tgtgtcgatt
     9121 tgtgaacgca aacgagaact tcgccgaaag cgccatcgcc gggaaaagta tcgcattttc
     9181 aagagaaaac ttttgacagc aaatgcctcg gagcgggcaa ttattgcgga aaaaattcga
     9241 aaattggccc ctgacccgga agtggtgttg aagaatctcg gcctcttgga gagccagcgt
     9301 tgattcgccc caggagcaaa ccttcttgtg acgctccttc ttttctgact cgccgcgaag
     9361 acggcccata agacgaaggc gagatcaaaa tggctattta ctcgacatga cgatcgttcc
     9421 gtcccaggaa ttgggcggaa cacggtgatg ttgtgcgatg tatgcggtca caaagtccgg
     9481 accgcggtcc tcggcaggca attgatgaaa gagggagaag gcccgctccc agtcgcctcg
     9541 gataaaggcc tccactgctt gttcacaaag gtggaggtgg tgctccttta agagaggata
     9601 ttcgcactcc gggggcagca gctcgtggac gaggaccgcc tgatccaaac catatggccg
     9661 aatccgcgcg atgggcctaa tccgtccgat ttctggagtc acgagagtcc tggcggctct
     9721 cgccgtctca tcgtccatca aaatcggaac atggatcagt ttggtgattc cctcaagacg
     9781 tgcggccaga ttgacaggcg gtccaaaaac ggtgattttt gcgtggtcgg gtgtgccaat
     9841 ctttccagcc acgacacgcc cgcttgctaa cccaatacca atgtgaaatc ccgcaagcgg
     9901 atgtccaggg gtctgcgccg cctgactgaa gtcgtgtcga atggcaaggg ccgcccgaca
     9961 ggctgcgagt acccgatcct cccgcggcaa cggccagccc caaaagccca tggcggcatc
    10021 cccctgaaag tccccaatga ctcccccatg ttcgaggatc cgccgggtca tcacctcgag
    10081 ggccgcacta atccggtcaa gaaggggcaa tagttgatca gcctggcgtt ccgtctcgtg
    10141 cgcaaatccc cgcaaatcgc aaaacaatac cgtggccacc acttcgcggg gttggagggc
    10201 ggcttcccaa tcctccggtc ccaggcgatc caacacgggc cggggcaaaa actgagagag
    10261 gatggcttgg cggtgctcca aacggcgaag ttgcaacagg gcacccgtca tttcagcgac
    10321 cagttccagg aacttagcat cctcttgaag ggtgtccggc gtggtggcac ccgatcccca
    10381 ttcagaatga aaccgaccgg ccacataaat cacccaagga gacgccccct cggccacaat
    10441 cggggtaccg atggcccagt ctatgttttg tcgcgccgtg taggtgggac ccgcacgcct
    10501 gtgggacggt tgactatgcg tggtttggcc cggtgacacg gtttttgagg taccttccgg
    10561 cactagttcg cgccacatgt gcaggacgct ttggcgacgc cgccaagcgt ccaggatgag
    10621 ctgccgactg ggacagaaat ctccctctgt cagttgtcgc cgatcccagt gaatcacacg
    10681 aatgtccaca tccaggggcg cgacttcttg ggccccgcca tcgtccgatg aacacgacgt
    10741 cgccggatag ggcttgtcca gggccacaat ggccacggcc tccacattcg gcatcccggc
    10801 caagagaaga ttgactagcc gggcagccag ttcggcgtcg gcagcagccc gggaaacgac
    10861 acggggcaat cgcaaaagga cttccagacg atagttcgga ttgcggaaaa cgacttgtcg
    10921 cagatactgc gggccaaagg ccttctgttg cgttggggtc gggtggtcgg ccgccacaga
    10981 aacccgctgc cgactgaggc taaagctcgt ttttccaatg acgaagcatt cacccggcct
    11041 gacccgaaat tcctcgcttt ccgt
//
LOCUS       DAOPTR010000006        10945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_5037_length_10945_cov_3.139027, whole genome shotgun sequence.
ACCESSION   DAOPTR010000006 DAOPTR010000000
VERSION     DAOPTR010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 10945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10945
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5037_length_10945_cov_3.139027"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PKI05_00225"
     CDS             complement(<1..380)
                     /locus_tag="PKI05_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50647.1"
                     /translation="MGDNDQKQDQPKPPPPPPPVLNQVILAVIALGAILSPVLLLYGS
                     GHIADGVARILSGEPIVAALGVRPATAPQDKPAAAGGENTFELQPVDFGEVFRFDITP
                     DWIVTRWPRVTASLSAIQLQGYRVP"
     gene            complement(605..1105)
                     /locus_tag="PKI05_00230"
     CDS             complement(605..1105)
                     /locus_tag="PKI05_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50648.1"
                     /translation="MVSSLGKPSLQNLLRLWTLHVLAAMLLGPLDSPCFGESWIFQPS
                     YFSHQEKGERVAQYAPPLPSVAPYAPNYVVSGYRHSKSTIRSAESYDHVHIVKTWGLG
                     AYIRPYEEWEYPFREGATPFGPWGNPQGPWTLPFQSWINPFALGRLPNPPWWIYPPYW
                     PPPPRP"
     gene            complement(1282..1941)
                     /locus_tag="PKI05_00235"
     CDS             complement(1282..1941)
                     /locus_tag="PKI05_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50649.1"
                     /translation="MGRCHRNRLFFGALAVMVVLMPLLLGCSQDSADSFRVKQSSPTA
                     STPPAPPPPPSASQPAPPASEPQRRPVAMPPGIARPEDVTSDFSSPAPAQPSGGMTPP
                     PPTSPQALPAATQGQYVRADVGVGQKGRTLERGFITTPVQVYFRSREHMAFRIQVPQA
                     LQLFQGEHGRLPESHEEFWREIIEKNGIQLPSLPPGHRYVYVPEKGELMVEVPAGSPP
                     P"
     gene            complement(1967..2827)
                     /gene="surE"
                     /locus_tag="PKI05_00240"
     CDS             complement(1967..2827)
                     /gene="surE"
                     /locus_tag="PKI05_00240"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOA50650.1"
                     /translation="MLILLTNDDGIYSPGLIALYEALQELGEVCVVAPATEQSGVGHS
                     ISYLRPLILKQVFHGDQHFGFAVDGSPADCVKLGVSELCPRRPDLVVSGINMGLNAGI
                     AVLYSGTVAAAIEGAFFGILSVAVSLQFDDNPQFSRAARLAVQIIQQILKQRSDEVRL
                     FNLNIPTKAVMDESPKVRVVSMDIRQYGEKYEKRVDPWGRTYYWATGEPSPTPEDRAT
                     DLSVLARGEIALTPLHYDLTAKSVLSKISEWEFPIRAEDVGSNVPGIPASVVVRTTHR
                     LQSVIPSKDQ"
     gene            3335..3871
                     /locus_tag="PKI05_00245"
     CDS             3335..3871
                     /locus_tag="PKI05_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50651.1"
                     /translation="MRWLVEDPTIPIVAIVIVEAVLAAFFVSTLKKAFLWAMAGMVVP
                     LAGILLADYLVVTDREAIETTIRKGVSALQANDLDQVLTFLSPSAVKTRERAEWAMSL
                     VTFTSVRISDLQIQVNKLTQPPTAEVRFFAVFRYEFKEPMPEAIHDIYAARFKVLMEK
                     VEDRWLLTDHYEYEIAHL"
     gene            complement(3916..4803)
                     /locus_tag="PKI05_00250"
     CDS             complement(3916..4803)
                     /locus_tag="PKI05_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50652.1"
                     /translation="MAAFWAGILMTGLTWGVNALGEEASGYRLRYRFQPGEELQWQVI
                     HRARVRTTVAQTEQTAETLTRSTKTWKVLDVDGNGSARFELRVEDVEMTNRVSDGNSN
                     GNEVRYDSKSGDPPPPGFADISKTIGKPLAIITLTSRGEVIDRKQLEPEALQTHGYLT
                     VPFPERPIKIGESWSVPDEVTIPLRNGTVKKVQIQQKFTLEDVKNDVAVIRIGTQVLT
                     PLNDPEVEVQLVQRLSEGTVRFDMAAGRMIAQEIEVDRQVVGFQGEASAFHYQTRFTE
                     EFLHSRMAQSPKPESTKQR"
     gene            complement(5037..5891)
                     /locus_tag="PKI05_00255"
     CDS             complement(5037..5891)
                     /locus_tag="PKI05_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOA50653.1"
                     /translation="MKVIVLGYGGRADVVKEVERFLPIIERYAEILLVDLSGEKDLSP
                     YQADYVIVFGGDGSILRAARQMGKKQFPVIAVNLGKLGFLADISPQELEGVLSLAEHS
                     RLPVVDHLMLSSELVAQGSVVVQSLALNEVAILNGPPFGLLDVELWVDGDLATVYSGD
                     GLILATPVGSTAHSLSAGGPILRKDLDAIVICPISPHTLSNRPVVDSADRVYELRVLN
                     PQPNTALVIDGCTVGHVRADHRIYVRRADPRFQLVSAPGHSYYRTLREKLGWGGQTRA
                     NNRSTSSS"
     gene            complement(5888..7831)
                     /gene="dxs"
                     /locus_tag="PKI05_00260"
     CDS             complement(5888..7831)
                     /gene="dxs"
                     /locus_tag="PKI05_00260"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008667679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOA50654.1"
                     /translation="MDKLLSKIQSPHDLQSLTSAQLEQLAAEMREAMCQLLGTRSAHF
                     ASNLGVVELTIALHTTFDFSRDRLIWDTGHQVYPHKMVTGRYRQFHTIRTKGGLMGYP
                     NPAESPYDLFMTGHAGCSISTAIGLKCGDDLLRPDENRYVVAVIGDGAFPSGIVSEAM
                     NHAGWLKKRLIVILNDNRMSICQRVGGIAEYLDRLRMAPLYSGIKNEVYNLLSKVPLV
                     GNSMERLLDQLRDAIKAGMLGGMLFEELGFRYLGPVDGHNIGQLQKYLRMVKQFPGPV
                     LLHVVTQKGRGFPPAEKDPRTFHAPAPFQRVNGTEVAFKKSGSARSYTEVARDAILKE
                     MQRNKQVVVITAAMCQGNMLEPVRDQFPDRYFDVGICESHAVGFAAGLAKAGLRPIVD
                     IYSTFLQRAYDQLFQEAALQNLPIVFMMDRAGVVGADGPTHHGVFDIAYFRHFPNMTV
                     LAPGDADELEAMLAVALQQEGPVAIRYPKATATVVSRPMVPIERGKAEWVVRGQDGTI
                     ICYGALLATCMEAVQRLNSQGLQFGLVNARFVKPLDADLICRVIQESPVVVTVEEGVL
                     QGGFGSAVLEAANDAGLNTSHLRRLGIPDRFIEHAERDELLSDLGLTAEGIAEACCAL
                     AAQVSRKPVRRSSARFSITGHHS"
     gene            complement(7831..>8120)
                     /locus_tag="PKI05_00265"
     CDS             complement(7831..>8120)
                     /locus_tag="PKI05_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOA50655.1"
                     /translation="SEVLNGLAEYGRAIGLAFQITDDLLDLLGDEKLVGKRLQKDNRQ
                     KKLTFPSLLGVEESRKRLDELIEAACRVLEGLPIERRHLVVLAINLKSRTH"
     assembly_gap    8121..8220
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8221..8885)
                     /locus_tag="PKI05_00270"
     CDS             complement(<8221..8885)
                     /locus_tag="PKI05_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009095365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOA50656.1"
                     /translation="MDFALNWQETEKHLAELRDLVNQWLDKLTDLPDGCPDRLKAAMR
                     YSLLAPGKRLRPTLVLLAAEACGADPEEAIAPACAIEMIHCYSLIHDDLPAMDNDDLR
                     RGQPTCHRQFDEATAILAGDALLTLAFETLSRYMEPADRAVQCCRLLAEAAGPCQLVG
                     GQADDIQSPTLTPTLDLLERIHRRKTGALIECALKMGGIVGSANSEVLNGLAEYGRAI
                     GLA"
     gene            complement(8976..9332)
                     /gene="xseB"
                     /locus_tag="PKI05_00275"
     CDS             complement(8976..9332)
                     /gene="xseB"
                     /locus_tag="PKI05_00275"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOA50657.1"
                     /translation="MNVSESDRCPSGEESTPRFEDALRELEAIVERLERGQLGLDEAL
                     AEYERGVRLIRHCRELLQKAERRIEILSGIDEQQRPITQPFEEEDLSLEEKREKRSRR
                     RGVKNLSDEESESEAG"
     gene            complement(9427..>10945)
                     /gene="guaA"
                     /locus_tag="PKI05_00280"
     CDS             complement(9427..>10945)
                     /gene="guaA"
                     /locus_tag="PKI05_00280"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOA50658.1"
                     /translation="LDFGSQYAQLIARRVREQNVFAVIVRHDISAERVQELAPKGLIF
                     SGGPASVHWSDSPKCDPDLFQLDIPILGICYGMQLVCQSMGGKVESDRGREYGPAICR
                     VIQQNELFTDLPSEFQVWMSHGDQVTSISEEFVVLAATESCPLAAVRHRSRPLYGLQF
                     HPEVTHTTYGSQILRNFVHKVCQCSGTWKLSDFAEETIKEIRQRVGRDRVICGLSGGV
                     DSSVTAALLSRAVGDQTACILVDNGLLREGEVESVIEEFTKHFQTDLHVVRAEDRFLA
                     ALRGVVNPQEKRKIIGRVFVDCFAEEAAKIPNARFLAQGTLYPDVIESGGLRDGPAAT
                     IKLHHNVGGLPEDLQFELIEPLRNLFKDEVRNLGMQLGLPDEIVWRHPFPGPGLAVRC
                     IGEVTKERLNILRQADAIVIEEIKRAGWYRKTSQAFAVLLPVQTVGVMGDARTYEEVI
                     AVRAVDTEDFMTADWSKLPYELLARISTRIINEVSGINRVVYDISSKPPATIEWE"
BASE COUNT         2532 a         3200 c         2761 g         2352 t
ORIGIN      
        1 ggaacgcgat atccctgcaa ttgaatcgcc gacaaactcg ctgtgaccct cggccagcgc
       61 gtcacaatcc agtccggagt gatatcaaac cggaagacct ctccaaagtc aaccggctgt
      121 agctcaaaag tgttttctcc cccggcagcc gctggtttat cctggggggc cgttgctggc
      181 ctgactccca gtgcggcgac gattggctcg cccgaaagga tgcgtgccac cccatctgca
      241 atgtgccccg aaccatacaa aagcaggacg ggcgaaagga tggcccccaa cgcaatgacg
      301 gcaaggatga cctggtttaa aaccggcggt ggtgggggcg gcggtttcgg ttggtcttgc
      361 ttctggtcgt tgtcgcccat cggttcaggt catttggcgg aaaggaagac tgctcaaaca
      421 attgcctcct ttcattgcct tcgggcgaaa cgggccccgc gctgcaagat ggcttcgcca
      481 accgtcaggt ttgtttgaaa tgttacctac gaaaaaactt tcgccaacag aacctgggtt
      541 gctcaattcg ccagaagcga cgttttatat cacgcgtaga tctcctggca cccccaacaa
      601 agtttcacgg gcgcggtggt ggtggccaat aggggggata aatccaccag ggagggttcg
      661 gcaaacggcc gagggcaaat gggttgatcc aggactgaaa cggcagcgtc cacggacctt
      721 gcggattgcc ccatggcccg aatggggtag ccccttcccg gaacgggtac tcccattcct
      781 cataaggacg aatgtaagcc cccaaacccc aagttttcac gatatgtaca tgatcatagc
      841 tttccgctga tcggatcgtg cttttgctat gtcgatagcc actgactaca taatttggcg
      901 cgtaaggggc aacggacggg agaggtgggg cgtattgcgc cacccgttcc cccttctcct
      961 gatgagaaaa ataggaaggc tggaaaatcc aactctcgcc gaagcatggt gaatcaagcg
     1021 gacctagcag cattgccgcg aggacatgta gggtccaaag cctcagaagg ttctgaagag
     1081 aaggtttgcc gagagaagac accatgaaag tctcgaactc gcaaaaacgc cgcccgcgac
     1141 ctcagatcac aatcaacggc cccatcatcg aggttaattg caggcgaaga gttttcaagg
     1201 cgagtccatc ccgaaaggca acttccgtca cgtctcaagc tcctcatgtg aggatgcccc
     1261 cgggtgctaa ttaacccctc tttatggggg aggtgaacca gccggaacct caaccatcag
     1321 ctcccctttt tccgggacat agacatatcg atgccctggt gggaggctag gcagctggat
     1381 gccatttttt tcgataatct cgcgccaaaa ctcttcgtgc gattcgggca ggcggccatg
     1441 ttctccttgg aagagctgga gggcctgggg gacctggatg cgaaatgcca tatgttctcg
     1501 actacggaaa tagacttgca ccggcgtggt gatgaatccc ctttccaaag tccggccttt
     1561 ctgcccaact cctacgtctg cccgaacata ctgcccctgg gtggccgccg gaagtgcttg
     1621 gggacttgtc ggtgggggag gcgtcattcc ccctgacggc tgtgccgggg ccggcgacga
     1681 gaaatcggac gtgacgtcct cgggacgcgc tataccaggc ggcatggcca cgggccgacg
     1741 ctgcggctca cttgccggag gagccggttg actggcggaa gggggcggtg gaggagccgg
     1801 cggcgtactc gccgttggcg aactttgttt gacccgaaaa ctatccgccg agtcctgaga
     1861 gcatcccaac aacaacggca tcaagacaac cataacggcg agagcgccga aaaacaggcg
     1921 attacgatga catcgaccca tggcgatctc ctgcacctgt gaatcctcac tggtcctttg
     1981 acggtatcac cgattgcaaa cgatgcgtcg ttcgcacaac caccgatgcg ggaatgcccg
     2041 gtacgtttga tcccacgtcc tcagcacgaa ttgggaattc ccactctgat atcttggaaa
     2101 gcacgctctt cgctgtcaga tcatagtgaa gcggtgtaag ggcgatctct cctcgtgcca
     2161 gaaccgacaa atcggtcgct ctgtcctcag gcgtgggact cggttccccc gtcgcccaat
     2221 aataggttct tccccaagga tcgacgcgct tttcgtattt ctccccatac tggcgaatat
     2281 ccatggaaac aacgcgcacc ttgggcgact cgtccatgac ggccttagta ggaatattga
     2341 gattgaaaag ccgcacctca tccgaccgct gtttaaggat ttgttgaata atctgtaccg
     2401 ccagccgggc agcccgcgaa aactgcggat tgtcatcaaa ctgcaaggat acggccacgc
     2461 tcaagatgcc gaaaaaagcc ccttcgatag cagctgccac tgttccagaa taaagcacgg
     2521 cgatccccgc attgaggccc atattgatac cgctgacgac aagatcagga cgacggggac
     2581 aaagctcgct aacccctaat ttgacacaat ccgccgggct tccatcgacg gcgaaaccaa
     2641 aatgctggtc accgtggaat acctgtttca gaatcaacgg ccgcaaatag ctgatggaat
     2701 ggcccacacc gctttgctca gtggccggtg caactacaca gacctcgcct aactcttgca
     2761 gtgcctcgta aagggcgatc aagcccggcg aataaatccc gtcgtcattc gtcaaaagga
     2821 ttaacacggt attgaccagt ctccccagaa acgggaaaaa gaacttggca ccttgtctgc
     2881 ggctatcaag ccaatataaa cttcgattac cttttcacta tatccggcgg taaccccagc
     2941 ggcaaccaat tagccgtaaa atcacggcgc ttgctaaagg tttggaagac ttcaaatggc
     3001 ttggctaaac gacatcgaat tctcgcttat agctcaacct tatgcccaga aaacaaatct
     3061 ccgtgctctt cattgcccca actccgacaa aactgtagga tcaagacaac gagttgcaag
     3121 cgagagatcg ccttttagac ttagaggcta tcccaaaaag tcgccctctc ccttcgggag
     3181 agggttgggg tgagggcaag cgaacgtcga aattaatcga gaaaaccggc aacgattcga
     3241 ggtaccatca tccccgaccc ctctcccgcc gaacgcgtga ggggagatta tgggatgggc
     3301 tcttaagtgg tccagtctgc gtggagtacc atttatgcgt tggcttgtcg aggaccccac
     3361 tatccccatc gtggccattg tcatcgtgga agcggtcctc gcggcgtttt tcgtatcgac
     3421 tctcaaaaag gcattccttt gggctatggc gggaatggtc gtcccgcttg caggtatctt
     3481 gttggctgac tacctcgttg tcaccgatcg ggaggccatt gaaacaacga ttcgaaaagg
     3541 tgtgagtgct ctgcaggcaa acgacttaga ccaggttctc acgttcctct ccccctcggc
     3601 tgttaagact agggagaggg cggagtgggc catgtccctt gtcaccttca ccagtgtccg
     3661 aatttcagat ctccaaattc aggtcaacaa gctaacccag cctccgacgg cagaagtccg
     3721 gttttttgcc gtgtttcgtt acgagttcaa agagcccatg ccagaagcca tccacgacat
     3781 ttacgcggca agattcaagg tgctgatgga aaaggtcgaa gaccgctggt tattaacaga
     3841 ccactatgaa tacgaaattg cccatctctg atacggcgtt ccactggtca gcgtgttttg
     3901 gaaaatagcc agcatctacc gttgcttcgt gctctctggc ttggggctct gggccattcg
     3961 gctatggagg aactcctccg tgaaccgcgt ctggtagtgg aaggcgcttg cctcaccctg
     4021 aaagccgacc acctgtcgat cgacctcgat ctcctgcgcg atcattcgtc cggccgccat
     4081 atcaaagcga acagtcccct cactgaggcg ctgaacgagt tggacctcaa cctcgggatc
     4141 attgagggga gtcaggacct gcgttccaat gcgaatcacg gccacgtcgt ttttgacgtc
     4201 ctcgagggtg aacttctgtt ggatttgaac ttttttcaca gttccatttc gtagcggaat
     4261 ggtgacctca tcgggaacac tccacgactc tcctatcttg atggggcgct ccggaaaagg
     4321 gactgtcaaa tatccgtggg tctgcaaagc ctccggctcg agctgtttcc gatcgatgac
     4381 ctctccgcga gatgtcaacg tgatgatggc cagcggttta ccgatggttt tgctgatgtc
     4441 ggcaaaaccc ggtggcggtg ggtcgccgga cttgctgtcg taccgcactt cgttgccatt
     4501 gctgttacca tcgctgacac gatttgtcat ttccacgtct tcgacgcgca actcaaatct
     4561 cgcagaaccg ttaccatcca cgtctaagac cttccaagtc tttgtcgaac gggtcagcgt
     4621 ctcggctgtc tgctccgttt gagctaccgt cgtccttacc cttgcccggt gaatcacttg
     4681 ccattgaagt tcctccccgg gttgaaagcg ataacgcaag cgataaccag atgcctcctc
     4741 gcctaacgca ttcactcccc aggttaaccc cgtcatcagg atgccggccc agaaagccgc
     4801 caatgcaact ccgcacactg atgaccggag ccacgagcca agagaacatc caaaagatac
     4861 catcatggca ataactcttt tctttcaatt ccgtgaaact tccacgccgc aggacgaaga
     4921 ccctccaagt cttcatgcct gtttataacc ctgtagcttg ggcctgaaaa gatcattttg
     4981 aaggccgcgc gatctcccct taacggcatc catggaagat caagaaactg ggcaacctag
     5041 gaagaggacg ttgaccgatt gtttgccctt gtctggccac cccagcccaa tttctcgcgg
     5101 agtgttcggt aatagctatg tccaggagca ctcactaatt gaaaccgtgg atcggctcgc
     5161 cggacgtaga tgcggtgatc ggcgcggaca tggccgacag tgcagccgtc gatcaccagc
     5221 gcggtgttgg gttggggatt caggacccgt aactcgtaca cacgatctgc cgaatcaacg
     5281 accggacgat tggaaagcgt gtggggacta atcgggcaga tcacaatcgc atctaggtct
     5341 ttgcgaagaa tgggaccacc tgccgaaagg gaatgggctg tcgaaccaac tggcgttgcc
     5401 agaatcagcc catctccgga gtaaactgtg gcgagatcac catccaccca caactcgacg
     5461 tcaagtaagc caaatggcgg accgttgaga attgccacct cattgagggc caatgattga
     5521 actacgacac ttccttgagc cacaagctcg ctggagagca tcaaatgatc cacgacgggc
     5581 aagcgactgt gctcagccaa agaaagcacg ccttccaatt cctgaggcga aatgtccgcc
     5641 aaaaacccca gctttcccaa gttaaccgcg attacgggaa attgcttctt tcccatttgt
     5701 cgcgctgccc gcaggatgga tccgtcacca ccaaaaacga tgacgtagtc ggcctggtac
     5761 ggagaaaggt ccttttcacc ggacaaatcg acgagcaaga tctcggcgta gcgctcgatg
     5821 atcggcagaa atcgttcaac ctctttgacg acatcagctc gaccaccgta gccaaggacg
     5881 atgactttca tgagtgatgc ccagtgatag aaaaacgggc ggatgaccga cgtacaggct
     5941 tccgcgacac ctgtgccgcc aaggcgcaac aggcctccgc aattccctcc gccgtcagac
     6001 cgagatcgga cagcagttcg tctcgttccg catgttcaat aaaccggtcg gggataccca
     6061 atctgcgaag atgcgaggta ttaagccccg catcgtttgc tgcttccagg accgcacttc
     6121 cgaatccccc ttgaagtaca ccctcctcca ccgtcacgac caccgggctc tcctggatga
     6181 cacggcaaat caaatcggcg tccaagggtt tgacaaaccg cgcattgacc agcccaaatt
     6241 gaagcccctg cgaattgagg cgctgcacgg cttccatgca cgtcgcaagg agcgcgccgt
     6301 agcatatgat ggtcccgtcc tggccacgga cgacccattc tgccttcccg cgttctatgg
     6361 gaaccatcgg ccgggagaca acggtcgccg tcgcttttgg gtatcggatg gcgacaggcc
     6421 cctcctgctg gagggcaacg gcaagcatgg cctcaagttc gtccgcatca cccggggcga
     6481 gaaccgtcat attgggaaaa tgcctgaaat aggcaatatc aaaaacccca tgatgcgttg
     6541 ggccatcagc cccaaccacc cccgcccgat ccatcataaa cacaatcggc aggttctgaa
     6601 gggccgcctc ttgaaagagt tgatcatacg cccgctgtag aaaggtgctg taaatatcca
     6661 caatcggccg tagccccgct ttggccagcc ccgcggcaaa gcccacggca tgagactcgc
     6721 atatgccgac atcaaaatat cggtcgggaa actgatcgcg gaccggctca agcatattcc
     6781 cctggcacat ggccgccgta atgacaacca cctgtttgtt tcgctgcatt tccttcaaga
     6841 tggcgtcccg cgccacttcg gtgtaagaac gtgcggaacc gctcttctta aaagccacct
     6901 ctgtgccgtt aaccctctgg aaaggcgcag gggcgtgaaa agtccgcgga tccttttctg
     6961 ccggcgggaa cccacggccc ttttgagtca cgacatgcaa caggaccggg ccggggaatt
     7021 gcttgaccat cctcaagtat ttttgaagct gcccaatatt atgcccatcc accggaccga
     7081 ggtaacggaa tcccaactcc tcaaaaagca ttccccccag catccccgcc ttaatggcgt
     7141 cgcgcaactg atcgaggagc cgctccattg agttcccgac caatggaact tttgacagaa
     7201 ggttatagac ctcattcttg atcccagaat acaggggggc cattcgcagt cgatcgaggt
     7261 attccgcaat gccgcccacg cgctggcaga tcgacatgcg attatcgttc aaaatcacga
     7321 tgagccgttt ctttagccaa ccggcatgat tcatggcctc tgaaacaatc cccgacggaa
     7381 acgccccatc cccaatcacg gccacaacat agcgattctc atcgggccga agcaggtcat
     7441 caccgcattt caaaccgatg gccgtcgaga tgctgcaacc agcgtgacct gtcataaaga
     7501 ggtcataggg gctctccgcc gggttggggt agcccatgag ccctccctta gtccggatcg
     7561 tgtggaactg acgataccgg ccggtcacca tcttgtgagg atagacctgg tgtccggtat
     7621 cccaaattaa tcggtcacgc gagaaatcaa aagtcgtgtg aagggcaatg gttaactcga
     7681 caacccccag atttgacgca aaatgggcgg agcgagtccc caacaactgg cacatcgctt
     7741 cgcgcatctc ggcggccagt tgttccagtt gggcactcgt caatgactga agatcgtgcg
     7801 gggactgtat cttcgacaga agcttatcca tcagtgggtc cgtgacttga gatttatggc
     7861 caagaccacc aaatgccgtc gctcaatggg taagccttca aggacgcggc acgcagcctc
     7921 aattaactca tctagccttt tacgactttc ttcaaccccc aacaaggacg ggaaagtcaa
     7981 ctttttctgc ctattatctt tttgaagccg cttaccaact agtttttcat caccaagcag
     8041 atcaagcaag tcgtccgtaa tttgaaatgc caacccgatc gctcgaccgt attccgcaag
     8101 accgtttaaa acctcagaat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 aatgccaacc cgatcgctcg accgtattcc gcaagaccgt ttaaaacctc agaattggcg
     8281 ctcccgacaa ttccgcccat ctttaaagca cactcgatca gtgctcctgt ctttctacgg
     8341 tgaatccgct ccagcaggtc aagtgtcggg gttaatgtgg gggattggat atcgtcggcc
     8401 tgtccgccca caagctgaca cggtccagca gcctccgcca gaaggcgaca gcattggacg
     8461 gcccgatctg ctggttccat gtaccttgaa agagtttcga acgcaagcgt caatagcgca
     8521 tcgccagcca ggatcgccgt cgcctcatca aactgtcggt gacaggttgg ctgtcctcgg
     8581 cggaggtcat cattgtccat ggccggcaag tcatcgtgaa tgagcgaata gcagtgaatc
     8641 atttcgatgg cgcatgccgg ggcgatagcc tcctcgggat cggcgccgca ggcctccgcc
     8701 gccaaaagaa cgagcgttgg ccgcagtcgt ttgcctggcg ctagaagact gtatctcatg
     8761 gcagccttca gtcgatcagg gcaaccatcc ggcaagtccg ttagtttatc gagccattgg
     8821 ttgaccaaat cacgaagctc ggccaaatgc ttttccgttt cttgccaatt gagcgcgaag
     8881 tccatgccga ccttgtcccc cgtggttgct gcgaatcgtg gataaaactc gtggataaaa
     8941 ccaatccctt tgcgctcccc tgccctagcg atctctcacc cagcctcact ctccgactct
     9001 tcatcggaaa ggttcttcac cccgcggcgg cgactccgtt tttctcgctt ctcttcgagc
     9061 gaaaggtctt cctcttcaaa aggctgcgtg ataggtcgct gttgctcgtc gattccagag
     9121 agaatttcga tccgccgttc ggccttctgc aaaagctccc ggcagtgccg aatcaggcgg
     9181 accccacgtt cgtactcggc caacgcctca tccaggccga gctgaccccg ttcgagccgt
     9241 tccacgatcg cctctaactc acgcagcgcg tcctcgaatc ggggtgtcga ttcctcacca
     9301 gaagggcagc gatctgactc actcacattc attcacttcc aaagatacca gcgtgctacc
     9361 tactccatcc atccaatgtg ttttttgccg cttctgcgac ctgcgttccc cctactctgc
     9421 ctaggcttac tcccactcaa tggtggcagg gggcttagaa ctgatatcgt aaaccacgcg
     9481 gttgattccg gaaacctcgt tgataatccg cgttgaaatc cgtgccaaca attcgtaggg
     9541 caacttcgac caatctgccg tcataaagtc ttcggtatcc actgcccgca cggctatgac
     9601 ttcctcatac gttcgggcgt cacccatcac acccactgtc tgaacaggca gcaacacagc
     9661 gaaggcctga gaggtttttc ggtaccaccc cgcccgtttg atttcttcga tcactatggc
     9721 atccgcttgg cggaggatat taagtcgctc ttttgtaact tctccgatac aacgaacggc
     9781 aagccctggc ccaggaaatg ggtgccgcca gacgatttcg tcgggcaaac caagttgcat
     9841 gccgaggttc cgcacctcgt ccttaaacag attgcgtaag ggttcgatca attcaaattg
     9901 caggtcttca ggcagtcccc caacgttgtg atgtaacttg atggtggcgg caggaccgtc
     9961 tcgcaatccc ccgctttcga tgacatcagg gtaaagcgtt ccttgggcca gaaaccgggc
    10021 attgggaatc ttcgctgctt cctcggcaaa gcaatccacg aacacacgtc caatgatctt
    10081 ccgcttctcc tgaggattca caactcctcg caaagccgcc aaaaagcgat cctctgcgcg
    10141 aacaacgtgc aggtctgttt ggaaatgctt tgtgaactcc tcgatgaccg attcgacctc
    10201 tccttcccta agtaatccat tatccacgag gatacaggcc gtctggtctc ccacagcccg
    10261 cgacaacaga gctgctgtca cggacgagtc cacccctccg gacaggccac aaatgacgcg
    10321 atcacgccca acccgctggc gaatttcttt gattgtctcc tcggcaaaat cgcttaactt
    10381 ccacgtcccg ctgcactggc ataccttgtg aacaaagttg cgcaggattt gcgaaccgta
    10441 agtcgtatgg gtgacctccg gatgaaattg aaggccgtag agtggccggc tgcgatgcct
    10501 tactgccgct aaggggcaac tttccgtagc tgccaggaca acgaactctt cggaaatcga
    10561 ggtcacttga tcgccgtggc tcatccaaac ttgaaactcg gatggcagat cggtaaacag
    10621 ttcattctgc tggatgaccc ggcagatcgc ggggccatat tcacgccccc gatcactttc
    10681 caccttacca cccatggact gacagacgag ttgcattcca taacatatcc ccaaaatggg
    10741 gatatcaagc tgaaatagat cgggatcaca ctttgggcta tcggaccagt gaacgctggc
    10801 cggcccgccc gagaaaatca gtcccttggg tgccaattcc tgaacccgtt ctgccgaaat
    10861 gtcatgacgg acaatcacgg caaagacgtt ttgttctcgg acgcgcctgg caatgagctg
    10921 cgcgtattgc gacccaaaat cgaga
//
LOCUS       DAOPTR010000007        10881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_5082_length_10881_cov_3.953168, whole genome shotgun sequence.
ACCESSION   DAOPTR010000007 DAOPTR010000000
VERSION     DAOPTR010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 10881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10881
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5082_length_10881_cov_3.953168"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2415
                     /locus_tag="PKI05_00285"
     CDS             <1..2415
                     /locus_tag="PKI05_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50659.1"
                     /translation="LVLRNDTNREVMNAEISWHLADQRAKQMVSKILPREEVDLKVPV
                     NTRLRPGHYSLHIVAKATQENRILEVNRDLTITIVPRPLPNTMPVIMWGTGDLDRVVD
                     IGFTHQLVSLADFDQIWKAETPTRAVPPGQLRERAEMLNEYLARGVGAIASLSPGSWV
                     VRNPKLQGQFQRVDRKGQPYKEANPCGLFPEVGAFCYRVGASGSETYRDFPAFQAALI
                     HTEVRDSTNLCFHEHDLRAFRTKTGLEIPDLATSKWGVLYEKIAEFPANRVIPDDHPL
                     RTFYAWFWSEGDGWNDLHSAVHRGLKSARPDVWTFFDPAVRAPSIWGSGGEVDVISQW
                     TYSYPDPIKIGQATDELFAMADGRPGQQVMKMTQIIWYRSQTAPNLPAEENRRAPWEK
                     EIPDARFITIAPDHLREAFWCKIARPIRGIMYHGWQSLVPAEHGSYRYTNPETKEVLK
                     DLIHRVVRPLGPTLLQVPDAPCDVALLESFTSQMFARRGTFGWGRGWEADMHLVLQWA
                     RLQPRIVYEQTILRDGLEGFKILVLPACDVLPASVVEKVKDFQAKGGVVVGDEFLCPA
                     ITPDIRITSYRRTGKAQEDKLALQEIADDLRGQLENRYARHFDSSHPDLVMRVRQFEK
                     TAYVFVVNDHRTFGDYVGHHGLVMEKGLPLEGEVRLARSSGVVYNLVEHRRVTNHAAG
                     SMLAWTVQLGPGDGRVFMVTDRPIGGLKIEVPDQARRGDRVAIALRVLDDQGQPMPAL
                     VPVELIVTDSQGQAAEPSGFYGAADGTVSVTLDLAPNDATGRWRIMARELASGQESSA
                     ELTVFE"
     gene            complement(2692..4128)
                     /locus_tag="PKI05_00290"
     CDS             complement(2692..4128)
                     /locus_tag="PKI05_00290"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOA50660.1"
                     /translation="MKNSRRSFLRCSMASPLFIASLGQRGTSAATTSLSEANSGSSVL
                     AEPARQIPVAGESDVIVCGAGPAGVAAAIAAARCGATVRLLEVHGCLGGIWTAGLLSW
                     ILDAGNKKGIIQEIIARLRMRDAVATYPEGASNAVGYRPEIMKWVLEEMIQEAGVCVQ
                     LFTRVCAAHVDDTKRLQAVITESKSGRQGWRAKVFVDATGDGDLAAFAGCRFDYGDEE
                     GGPVQPMSMLAMLVGLDPEAVKPFVRGLAEPDGERNPKGRLLEEIRRGGHDPSYHAPT
                     MFYLGHGLFCLMANHEYRVSALDAAEITAATFRSRAEVHRIVEALRSLGGPWKEIQIV
                     ARNEQIGVREGRRPYGLYRLTAEDLKNGTRHEDAVCTVTFPVDIHSPDPEKNKGIVAR
                     GFRSQPYDIPYRALVAADVRGLLFAGRCISGDFVAHSSYRVTGNAAAMGEAAGVAAAL
                     AAAHHCDPKDVPWSEIASQLERLRSQAG"
     gene            complement(4331..5719)
                     /locus_tag="PKI05_00295"
     CDS             complement(4331..5719)
                     /locus_tag="PKI05_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA50661.1"
                     /translation="MSRPVSGSSRREFLKTTGRLAAATALAGSMIPHVHAAEDNTLRI
                     ALIGCGGRGTGAAANAMSVKRVPIKLVAMADVFPNRLASSYEQLKNRFGDQVDVPDDR
                     KFIGFDGYRKAMDCLRPGDVAIFATPPAFRWVHFTYAIEKNLNVFMEKPVTVDGPTSK
                     RMLELAQKSVEKNLKVGVGLMSRHSRALQELFKRIQDGAIGDVIFQRGYRMHGPIGFF
                     ASTPKPEGISELLYQVQRFHSFIWASGGCYSDFYIHIIDHLAWMKNDWPVKAMGLGGR
                     HYKTSPEGVPYIDQNFDVYAVEYTYADGSKFCFDGRCMGGADNVYYSYIHGSKGSALV
                     SASGDCGLPSRIFRGQNPDMSALEWESKVPPEERDPYQNEWNALVDAILDDKPHNEAE
                     RGVKASLVTSMGRMACHTGREITYEEILNHSHEMAPGLDQLTMDSPAPLQLGPDGRYP
                     VPMPGLKTDREY"
     gene            complement(6131..6640)
                     /gene="coaD"
                     /locus_tag="PKI05_00300"
     CDS             complement(6131..6640)
                     /gene="coaD"
                     /locus_tag="PKI05_00300"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOA50662.1"
                     /translation="MSELNSRVAVYPGSFDPITLGHLNVIERASRLFDQLIIGIGVNI
                     HKFPMFDAKEREELIRLSTPHLTNLEIRTFRGLAVNFVKECGARIMIRGVRPITDIAA
                     ELTMMMANRRLAPDVETLFMIADGELAHVSSSLIKEIAPVASDEELSRFLPDPVVRAL
                     RAKLQASRR"
     gene            6778..8553
                     /locus_tag="PKI05_00305"
     CDS             6778..8553
                     /locus_tag="PKI05_00305"
                     /inference="COORDINATES: protein motif:HMM:NF037334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50663.1"
                     /translation="MGSTKIVLGASINLTRSAMWLSVLVMTWGVLGSSVYAQRQMDKL
                     DRGLVAVQAGPGEVYVSWRLLKDDPKDLAFNVYRQAEGTAQAVRLNDRPISKTCDFTD
                     RGVSRGRYVYSLRAVKGGKEEQSGPSFTLEVTDKPQPYVSIKLQGDHTFQKVGIADLN
                     GDGSLDIVIKQPNQNIDPYEKYWKPSPSTYKLEAYTLDGKMRWRYDLGWAIEQGIWYS
                     PYIVYDLDGDGKAEVAVKTGEGDPRDSDGRVQSGPEYLTILDGETGKPVTRTDWPSRE
                     LFPSYNYASRNQLAVAYLDGKTPSLIVLRGTYNVMLAIAYRYEKGRLTEIWRFDNRGM
                     ERKFQGQGAHWTHAADVDGDGKDEIILGSVVLDDNGQPLWSTGLGHPDHAYVGDLDPA
                     RPGLEIYYGIETRQQKNGMCMVDARTGEILWGYDGPTRHVHSHGMCSDIDPRYPGAEC
                     YSADTDANKDFAWAKLWSAKGEVISEENISGFGPRVAYWDADPQRELVMGSRIRKYHP
                     SQELSPRLEGSFVATVDLLGDWREEIITTLPGEMRIYVTTIPASDRRPCLLQDPIYRL
                     DVAMAAMGYYQCPMTSFDLASQKAE"
     gene            8631..9425
                     /gene="larB"
                     /locus_tag="PKI05_00310"
     CDS             8631..9425
                     /gene="larB"
                     /locus_tag="PKI05_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007335257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOA50664.1"
                     /translation="MLNIEQLRILTEQLKRGQIDVEGFVQACQKWGIADVGEAQVDID
                     RQRRCGFPEVVYGEGKSVEALRKIFHALCENGVDVLATRVSQEKADALLPEFPNARYN
                     ALAKTFRIAREAKTEDQPRGIVTVVTAGTSDLPVAEEARETALWTGAEVRLVQDVGVA
                     GPHRLRANLHLLQDADAIVVVAGMEGALPSVVGGFVSCPVIGVPTSVGYGASFGGVSA
                     LLSMLNSCAANVVVVNIDAGFKAGYVAGLIAKNSALSRAKSQSAAK"
     gene            9422..10324
                     /locus_tag="PKI05_00315"
     CDS             9422..10324
                     /locus_tag="PKI05_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOA50665.1"
                     /translation="MIQVPFEDNPHLPVLPIRPRDAHKGHFGTVLIVGGSLGMTGAAG
                     LAGMAALRGGAGLVRVAVPEPVLPIVAGYEPSYTTIPLPADSAGRISYQALKKIRVEA
                     ELADWVALGPGLGRSWGLSRLVSQLYRDLPNPLVVDADGLNALVTLAPDIPPANGPRI
                     LTPHPGEFRRLVGPGADPRAPLADRWQLAQHYAAVWDAVVVYKGHPTLVTDGKRRYFN
                     PTGNPGMATGGSGDVLTGLIAALGAQGLDSFSAAQLGVYLHGLAGDLGAKDLGEESLI
                     ASDLLRYLPLAFQKYRETSSKTNG"
     gene            10450..10863
                     /locus_tag="PKI05_00320"
     CDS             10450..10863
                     /locus_tag="PKI05_00320"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HOA50666.1"
                     /translation="MQIYAYLADLVVALHLAYVLTVIVGLVLILVGWLAKWRWIRHFW
                     LRLIHLLMIAVVAFQAWIGVICPLTHLENYLRKLAGQSGYSGTFIGHLLESLLYYEAP
                     PWVFVFAYTLVAVLILSTLILVPPNWPRRLKPSQQ"
BASE COUNT         2339 a         3021 c         3156 g         2365 t
ORIGIN      
        1 ctcgtcctgc gcaacgatac aaaccgggaa gtgatgaatg ccgaaatctc ctggcatttg
       61 gcggatcaac gggcgaagca aatggtgtcc aagattcttc cacgtgagga agtcgatctc
      121 aaggtccccg tgaatactcg cttgcgtccg ggtcactatt cgctccacat tgtggcgaaa
      181 gcaacgcagg agaatcgaat cctagaagtt aatcgggacc tgacaataac catcgtgccc
      241 aggccccttc ccaacaccat gcccgtgatc atgtggggga caggggacct cgatcgcgtg
      301 gttgatattg gttttacgca tcaactggta agcctggcgg acttcgatca gatttggaaa
      361 gccgagacac caacgcgggc tgtaccaccc gggcagctca gggaaagggc ggagatgctt
      421 aatgagtatt tggcacgagg tgtgggagcg atcgctagct tgtcgccagg gtcgtgggtt
      481 gttcgcaatc caaagttgca aggccaattc caacgggttg atcgaaaagg acaaccctac
      541 aaagaggcca atccttgcgg tctgtttccc gaggtcggcg ccttttgcta tcgcgtgggg
      601 gcatcaggga gcgagacata tcgtgacttt cccgcttttc aggcggcgct cattcacacc
      661 gaggtccgtg acagcaccaa tttgtgtttc cacgaacacg acctccgggc gtttcggacc
      721 aagaccggtt tggagattcc tgatcttgcc accagtaaat ggggcgtgct ctacgagaag
      781 attgccgagt ttccagcaaa ccgcgtgatt ccggatgatc atccgttgcg aactttttat
      841 gcatggtttt ggagtgaagg tgacggctgg aacgacttgc attcggcggt ccatcggggc
      901 ctgaaatcgg cgcggcctga cgtgtggaca ttttttgacc ctgccgttcg tgcccccagt
      961 atttggggca gcgggggcga agtggacgtc atttctcagt ggacatacag ctatcccgac
     1021 cccatcaaaa ttgggcaggc tacggatgaa ctgttcgcaa tggctgatgg ccgtcccggt
     1081 caacaggtga tgaagatgac gcaaatcatc tggtatcgtt cgcagaccgc tccgaattta
     1141 cccgccgagg aaaaccgccg tgccccttgg gagaaggaga ttcccgacgc gaggttcatc
     1201 accattgcgc cggaccatct tcgcgaggcg ttttggtgca agatcgcccg gcccatccgt
     1261 ggcattatgt atcacggatg gcaatcgctc gtaccggcag aacatgggag ctaccgctac
     1321 accaacccgg agacaaagga ggtcctgaaa gacctcatcc accgtgtagt gagaccgctg
     1381 ggaccgacac tgcttcaggt tccggatgcc ccatgcgatg tagcccttct ggagagtttc
     1441 acttcgcaaa tgttcgccag acgcggcacg ttcggttggg gacggggctg ggaggccgat
     1501 atgcacctgg tccttcaatg ggctcgcttg cagccacgga tcgtctatga gcaaacgatc
     1561 ttgcgcgatg ggcttgaagg attcaaaatt ctcgtcctgc ctgcgtgcga tgtcctgccg
     1621 gcatcggtcg tggagaaggt caaggacttt caggcgaaag gtggtgtggt cgtgggcgat
     1681 gagttcctat gtcccgcgat cacccccgat attcgaatta cttcctatcg gcgtacgggc
     1741 aaagcccagg aagacaagct ggccctgcaa gagatcgcgg acgacctccg gggtcaactg
     1801 gagaaccgct acgcgcgtca cttcgattct tctcaccccg atctggtgat gagagtgcgg
     1861 cagttcgaaa agacggcgta tgtgttcgtg gtgaatgacc atcgcacatt cggcgattat
     1921 gtgggtcatc acggattggt catggagaag ggactcccat tagaaggtga ggtccgattg
     1981 gcgaggtcat ccggtgtggt ctataacctt gtggagcatc gtcgagttac aaaccatgcg
     2041 gccgggtcga tgcttgcttg gacggtacaa cttgggccag gcgatggccg ggttttcatg
     2101 gttacagatc ggcccatcgg cgggttgaaa atcgaggtgc ccgatcaagc ccgcaggggc
     2161 gaccgcgtag ccattgccct tcgcgttttg gacgatcagg gccagcccat gcccgcgctc
     2221 gtgcccgtcg agctcatcgt caccgactct caagggcaag ctgcggaacc gagcggcttc
     2281 tatggtgccg ccgatggcac ggttagcgtc accctggatt tagccccgaa tgatgccacc
     2341 ggcagatggc gaattatggc gagggaactg gcctccggtc aagagagctc cgctgagctg
     2401 acggtctttg agtgacttct cacccgcagt gcaacttagt gcaacttaga gcctatccca
     2461 caacctcccc tcgcccgttc ggcgggagag gggccggggt gagggtggct cgaatcgctg
     2521 ctggtttttt ggatcgactc cgacgatcgt ttgccctcac cctaaccctc tcccgggggg
     2581 agagggaact ttgggacagt ctccaaggct atcgcttcac gtgagcgagc agcagcgcac
     2641 ggccggaccg gttgggcact cgctgccgat tttaaggctt cggcatcgct attacccagc
     2701 ctgggaacgt agccgctcta actgcgaggc aatttccgac cagggaacat ctttggggtc
     2761 gcaatgatgg gccgcggcaa gcgcagcagc cacgccggcg gcctcaccca tagcggcggc
     2821 gttacccgtc acgcggtagc tggaatgagc gacgaaatcg ccgctaatgc atcgccctgc
     2881 aaataaaaga ccacggacgt cagcggccac caatgctcgg tacggaatat catacggctg
     2941 ggagcgaaaa ccccgggcga cgatgccttt attcttttcc gggtcgggcg aatgaatgtc
     3001 cacgggaaag gtgaccgtgc agacggcgtc ttcgtgccgc gtcccgttct tgaggtcctc
     3061 ggcagtcaaa cggtacaaac cgtagggacg gcggccctcg cggacgccga tctgctcgtt
     3121 tcttgccaca atttggattt ccttccatgg cccgccgaga gatcgcaagg cctccacgat
     3181 ccgatgaact tcggcccgcg aacgaaacgt ggcggctgtg atttcggcgg catcgagggc
     3241 ggatactcgg tactcgtgat tggccatcag acagaacaga ccgtgcccca gataaaacat
     3301 cgtcggggcg tggtaagacg gatcgtggcc gccgcggcgg atttcttcga gcagccgtcc
     3361 cttcgggtta cgttcgccgt caggctcggc aaggccccgc acaaaaggct tgaccgcctc
     3421 cggatcaagt cccactagca tggccagcat actcatgggt tggactggcc ctccttcttc
     3481 gtccccatag tcgaatcggc agcccgcaaa ggcagctaga tctccgtcgc cggttgcgtc
     3541 aacgaacacc ttcgcccgcc agccttggcg tccagacttg gactcggtaa tcacggcctg
     3601 caaccgcttg gtatcatcca cgtgcgcggc gcagacccgc gtaaaaagtt gcacgcacac
     3661 gcccgcctct tggatcatct cttcgagtac ccatttcatg atctccgggc gatagccgac
     3721 ggcattgctg gcgccctcgg gataagtggc aaccgcatcc cgcatgcgga ggcgggcaat
     3781 gatttcctgg ataattccct ttttgtttcc cgcatccaaa atccaagaga gaaggcccgc
     3841 agtccaaatg cctcccaggc atccgtgaac ttccagcagt cgcaccgtgg cgccgcaccg
     3901 tgcagcggct atcgccgcgg caacaccggc cgggcctgcc ccgcaaacga tgacatcact
     3961 ttccccggcc acaggaatct gccgggcggg ttcggcaaga acagaactac cggaattcgc
     4021 ctcggacaaa gatgttgtcg ccgcactggt tccacgttgt cccagcgaag caatgaaaag
     4081 gggacttgcc attgaacaac gcaaaaagct ccgccgcgag ttcttcatcg ttataccttt
     4141 cctgatgcgt tgacaacacc agtgtcgctt gaaggcttcc gctatgcatc agatcgcaca
     4201 aaaaaggatg ataagaacac accggtgtgc tcttatcatc caaatctcca tgcgcaagtc
     4261 aagctttgac acggcgccca cgccggggat atccgttttg ggaatccaca atgatcctgt
     4321 cgcttcgaaa tcaatactca cgatcggttt tcagaccagg catggggacg gggtagcggc
     4381 cgtcggggcc aagttgcaac ggcgccgggg agtccatcgt caattgatcc aggccaggcg
     4441 ccatttcgtg ggaatgattc aggatttcct cataggtgat ttctcggcct gtatgacaag
     4501 ccattcgccc catcgaagtg acaaggctgg ccttcacgcc gcgttcggcc tcgttgtggg
     4561 gcttgtcgtc aagaatggcg tcaaccaggg cattccattc gttttgatag gggtcgcgtt
     4621 cttcgggcgg gaccttggat tcccattcca gggccgacat gtcaggattc tgtccccgga
     4681 aaatccggga cggcagcccg cagtccccgc tggccgagac caaagccgac ccttttgaac
     4741 cgtgaatgta gctgtaatac acgttgtcgg ccccgcccat gcaacgaccg tcaaagcaga
     4801 acttgcttcc gtcggcatag gtgtattcca cagcgtacac gtcgaagttt tgatcgatgt
     4861 agggcacacc ctcggggctt gtcttgtagt ggcgaccgcc gagacccatg gccttgaccg
     4921 gccagtcgtt cttcatccag gccaggtgat cgatgatgtg aatgtaaaaa tcgctatagc
     4981 aaccaccgct tgcccaaatg aagctgtgga atcgctgcac ctgatagaga agctcgctga
     5041 ttccctcggg ctttggggtt gaagcaaaga atccgatggg gccgtgcatg cggtatccgc
     5101 gctggaaaat cacatcgccg atggcaccgt cttggatgcg tttgaacagc tcttgcaatg
     5161 cccgactgtg acgggacatt agccccacgc cgaccttaag gttcttctcg acagatttct
     5221 gagccagttc gagcatccgt ttgctggtag gaccatcgac cgtgacgggc ttttccatga
     5281 agacgttcag gtttttctcg atggcatagg tgaaatggac ccagcgaaac gccgggggcg
     5341 tcgcaaagat cgccacatcc ccaggccgca agcaatccat ggccttgcga taaccgtcaa
     5401 acccgatgaa ctttctatca tcaggcacat cgacctgatc gccaaatcgg tttttcaact
     5461 gctcgtaact agaagccagt cgattgggga aaacgtcggc catcgctacc aatttgatgg
     5521 gcacccgctt tacagacatg gcattggcgg cggccccggt tccgcgtccc ccgcaaccga
     5581 tgagggcgat gcgaagggta ttgtcctcgg cggcgtggac atggggaatc atgcttcccg
     5641 ccagcgccgt ggctgctgcc aaccggccgg ttgtcttcaa gaattcacgt cgcgacgaac
     5701 cactgacagg acgactcata agaaacctcc tcggcgttca gaacggttca gaaactgggt
     5761 agctgatggc aacaattgaa ggcaggccct ggatcttcgc caagggtcag tctttcagcg
     5821 tccccaaaac agaataccac gattaaggct gtattgtcat ctattgaacg gcgatgtcaa
     5881 tgggttgcga tgctctgtca ctcgcgtcct ttctggatgg tgtccgcccc caaagcacgt
     5941 gacgaggcgt tttcctcaag aagatttgct gctggagcga tagaggccgc aatggaccgc
     6001 ggcgcgaggt cgtacccaat tcaacgacag aggctcacgc actggtatgg tcgtggaacc
     6061 ctaaagaact cagcactcgt ttaccaacgc gaagaccttt cagtaaatct ttccgacagt
     6121 ttagcttggc ctatcgccgt gaggcctgca attttgcccg taaagcccgg acaacaggat
     6181 caggtagaaa tcgggaaagc tcttcgtcgc tcgctacggg ggcaatctcc ttgatcaacg
     6241 aactggagac gtgggcaagc tcaccgtcgg caatcatgaa cagtgtctca acatccggtg
     6301 ccaaccgccg gttggccatc atcatcgtca attccgctgc gatgtcggta attggccgca
     6361 caccacgaat catgatcctg gcgccgcatt ccttcacaaa attgacggcc aagccccgaa
     6421 acgtccgaat ctccaggttc gtcaaatggg gcgtcgaaag tcttatcagt tcctcccgtt
     6481 ccttggcgtc aaacatcggg aacttgtgaa tgttgacacc aatgccgatg atgagctggt
     6541 caaacaagcg gctggcgcgc tctatgacat ttaggtgacc cagcgtgatt gggtcaaagg
     6601 aaccgggata gacggcaact cgcgaattaa gctcagacat aagataagct cctcaggcgg
     6661 tagcaaaaca cctctttggt gattatatta cgaaagaact tctgtgccga gaggtaagag
     6721 gcatcgatag gtttgacgac aatcttgaat tcccacaatt gcggggagga ggatgcaatg
     6781 ggttcgacaa agatcgttct tggcgcttcg attaacttga cgcggtccgc aatgtggctc
     6841 agcgttttgg tcatgacctg gggtgttctg gggtcgtcgg tttatgccca gcgacaaatg
     6901 gataaactcg accgcggctt ggtagcagtg caagctggtc ccggggaagt ttatgtgagc
     6961 tggcggcttc tgaaagacga cccgaaggac ctggccttta atgtttaccg gcaagctgag
     7021 ggaacagccc aggcggtccg cttgaacgat cggcccattt caaagacctg tgattttacc
     7081 gatcgcgggg tctcgcgtgg gcggtacgtc tattctcttc gagccgtaaa aggcggcaaa
     7141 gaagaacaga gcggtccatc attcaccctg gaagtgacag ataaacccca accttatgtg
     7201 tcgatcaagc tacaaggtga tcacacgttt caaaaggtag gtattgccga cttgaatggc
     7261 gatggatcac tggacatcgt catcaagcag cccaatcaaa atattgatcc ctatgaaaag
     7321 tattggaagc cgagccccag tacgtacaaa ttagaggctt acaccttgga tggcaaaatg
     7381 cggtggcggt acgatcttgg ctgggccatc gagcagggca tttggtactc gccctatatc
     7441 gtgtatgacc tcgatggtga tggaaaggcc gaggttgcgg tgaaaaccgg tgaaggtgac
     7501 ccgagggatt ccgatggccg tgtgcaaagt ggcccagagt atctgacgat cttggatgga
     7561 gagacaggca agcctgtgac acgaacggat tggccgtccc gagagttatt cccctcgtac
     7621 aattacgctt cccgtaatca gcttgctgtg gcctaccttg acgggaaaac tccatcatta
     7681 atcgtcttgc gcggaacata taacgtcatg ctggccattg cttatcgcta tgaaaagggt
     7741 cggctcacgg aaatctggcg ctttgataac cgtgggatgg agcgaaagtt ccagggccag
     7801 ggtgcccact ggacacacgc tgccgatgtc gatggtgacg gtaaagacga gattatccta
     7861 gggtcagtgg tccttgatga taatggccaa ccattgtggt caacaggtct gggacatccg
     7921 gaccatgcgt acgtcggcga tctcgatccg gcacggccag gactggagat ttattatggg
     7981 atcgagactc gccagcagaa aaacggcatg tgcatggtcg atgcccgcac aggtgagatt
     8041 ctttggggct acgacggtcc aacgcggcac gttcatagtc acgggatgtg cagtgatatt
     8101 gatccccgtt atccgggcgc cgaatgttac agcgccgata cggacgccaa caaggatttc
     8161 gcttgggcaa agttgtggag cgccaaaggt gaagtcatca gtgaagaaaa catttccggt
     8221 tttggccccc gcgtggccta ctgggacgcc gatccgcagc gggaactggt gatgggctct
     8281 cgaatccgca agtaccaccc gtcgcaagag ctttcacctc gtctagaagg gagtttcgtg
     8341 gccacggtcg atcttcttgg cgactggcgt gaagagatca ttacgacgct ccccggcgaa
     8401 atgcggatct acgtgaccac gatccccgca agcgaccggc ggccgtgcct gctccaggat
     8461 cccatctatc ggctggatgt tgccatggcg gcgatgggat attatcagtg ccccatgacc
     8521 agtttcgatt tggcatctca aaaagcggag tgaccgtcat tttaggagac gatccctgat
     8581 cgttcaaaaa gggtatttgc gacattgttg tcaacaaatc actgccaatc atgttaaata
     8641 ttgagcaact acggatactg accgagcaat tgaagcgcgg ccagatcgac gtggaaggtt
     8701 ttgtccaggc gtgccaaaag tggggaatcg ccgatgtcgg tgaggcccag gtggacatag
     8761 atcgccagcg gcgctgcgga ttcccggagg tggtctatgg cgaaggcaag agtgtcgaag
     8821 ccctgcgcaa gatcttccat gccctgtgtg aaaacggtgt cgatgttctc gccacccgag
     8881 tttctcagga gaaggccgac gccttacttc cggagttccc caacgcccga tacaacgctt
     8941 tggccaaaac attccggatt gcccgggagg caaagactga ggaccagccg cggggaattg
     9001 tgaccgtggt aacggccgga acaagcgatt tgcccgtcgc agaagaagcc cgagagacag
     9061 ccctgtggac cggtgcagag gttcgattgg tccaggatgt tggggtggct gggccgcacc
     9121 gcctccgggc aaatctgcac cttcttcagg atgccgacgc cattgtcgtc gtggcgggca
     9181 tggaaggggc gctgcccagc gtggtgggag ggttcgtttc ctgtcctgtt attggtgtac
     9241 ccacgagcgt tggctacggg gccagtttcg gcggcgtctc cgcactgctg agcatgctga
     9301 atagctgcgc ggccaatgtg gttgtggtga acatcgacgc cggcttcaaa gctgggtatg
     9361 tggcaggttt gattgccaaa aatagtgccc tgtctcgcgc aaaatcccaa tctgccgcaa
     9421 aatgatccaa gtaccctttg aagacaatcc ccacttaccg gtattgccga tccgtccgcg
     9481 agacgcccac aaaggtcact tcggcaccgt gttaatcgtc ggcggaagtc tcggtatgac
     9541 gggggcggct ggcctggctg gaatggcggc cctacgaggc ggtgccggat tggtccgtgt
     9601 cgcggttccc gagccggtgt taccgattgt cgccgggtat gagccgtcgt acaccaccat
     9661 cccactcccg gccgattcgg cgggtcgtat ttcctatcag gctctgaaga agatacgtgt
     9721 ggaggccgaa ctggcggatt gggtggcact aggaccggga ctgggccgtt cgtgggggct
     9781 atcgcggctt gtctcccagc tataccgcga cttgcccaac ccattggtgg tggacgccga
     9841 cggcctgaat gctctggtga cactggcccc ggacataccg cccgccaacg gcccgcggat
     9901 tttgacgcca catccaggcg aattccgccg attggttggg ccaggtgccg atcctcgagc
     9961 gccactggct gatcgctggc agttggccca gcattacgca gccgtctggg atgcggtggt
    10021 ggtctataaa ggccatccaa ccttggtgac ggacggaaaa cgtcgttatt ttaacccaac
    10081 cgggaatcct ggcatggcca cgggtggatc gggagacgtt ttaaccggtc tcattgccgc
    10141 cttaggagcg cagggactcg atagcttttc tgccgcccaa ctaggcgttt atcttcacgg
    10201 tttggctggg gacctgggcg ccaaggacct cggcgaagaa tcgctgatcg cttcggatct
    10261 cctgcggtac ctcccgctgg catttcaaaa gtatcgagag acatcctcca agacgaacgg
    10321 ataaacggaa gggcccgatc accgtttcgc cggcttcgct tgccaaccga ggcacggtcg
    10381 cctggaataa atcccccgcc aaatggatcg aaattatagc tccatacttg acgccgggat
    10441 gctgcggata tgcaaatcta cgcctatctt gccgatcttg tggtggcgct ccatttggcc
    10501 tacgtgctaa cggtgattgt cggcctggtc ctcatcctcg tcggctggtt ggctaagtgg
    10561 cgatggattc gccatttttg gctccgcttg attcacctgt taatgattgc cgtcgtcgct
    10621 tttcaggcct ggatcggtgt gatttgcccg ttgacccacc ttgagaatta tttgcggaaa
    10681 ctagccggac aatccggcta cagcgggacg tttatcggcc acctgttgga atccttgctc
    10741 tattacgaag cacccccgtg ggtctttgtg tttgcctaca ccttggttgc ggtgctaatt
    10801 ctttccacac taatcctggt tccccccaac tggccaaggc gtcttaagcc ttctcagcag
    10861 taggccggtg ttgattggct t
//
LOCUS       DAOPTR010000008        10876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_5088_length_10876_cov_3.292394, whole genome shotgun sequence.
ACCESSION   DAOPTR010000008 DAOPTR010000000
VERSION     DAOPTR010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 10876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10876
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5088_length_10876_cov_3.292394"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(706..1764)
                     /locus_tag="PKI05_00325"
     CDS             complement(706..1764)
                     /locus_tag="PKI05_00325"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOA50667.1"
                     /translation="MTCTRRSFLQQGAALLGYLAGGLENRFSRGSLGLEASETVPELT
                     TIPIVDTHQHLWDLSKIRLPWLAGDERLNRNYLVEDYLEAVKGLPVVQAVYMEVAVAD
                     EDLVKEAEWIIEICRSGRGPTVAAVIGGRPASDDFRTYLARFRNNPVVKGVRWIPHNS
                     KLGHKLYFGPQMRDNLRLLGEWGMSFDICIPPDWLSDAAELVDACPDTRFIIDHCGNA
                     DPQQFGRWGKERGEEATRYVEQWRRAMDGLAARKNVVCKISGIIARVRPDDWRPDDLA
                     PIVNHCLEAFGSDRVMFAGDWPVCTKGAPLRAWIAALREITASRSLEERRKLFHDNAV
                     RFYGLEPIPKFPRPLGGG"
     gene            complement(2136..4718)
                     /locus_tag="PKI05_00330"
     CDS             complement(2136..4718)
                     /locus_tag="PKI05_00330"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOA50668.1"
                     /translation="MTRIALLAIDVTNCGPWRGHHRLEFPATGVAVFCAPNETGKTTL
                     LRLVAAVLWGSGVPECNWFAGEDESFAAAIEYERAALQPRHETEPPIRYRVERDFRTG
                     RVCLFRLEKGEWQSVQVTRHKRRGRTADSEQWMNLVQEVFAPISPRAFERLVVLSPPF
                     NPQPEGELVQSLISGAGESTKDEALETLLERYRAITRYSKKEGLSTSDARKDGRLDEL
                     RRRRDELQRDCELAKEMLEKGLRLREEIQRLDEQVAAKEEKRNELQRNKGILDKTRRL
                     SAERRTKEELAGNLARALDQWKKLEDSAREVREQLQRFPAFLRDSTSERRKYWAEALR
                     KYQERARRLIEQSRVVNEEVLKERFADVWDWPADAPSRVEQIQKALALCHEAEQHHRE
                     AVSRAASIRPVLDRKRQMPVVIGVAAAVMLVLVLILGLFGYLVMGLLVGVIAAIAGGL
                     ITAQIYRPECWPPEYAQWQQKVAQAEGDHEKCVRALEKYWEEIARWAQTRELPILLER
                     LGRFKSLCDAKRQLLNQKAELEQVAAQLTVRAIPKELTDMCGLESEAADSAPLGPDVF
                     ERGLSLLSEYEKLVAHEQSYRQQQKIVLQSVGVDTIESLQERHRQAEGDLQGIILESR
                     KLAQDSPLAEEAFSWDARRLENEYRRLEEELTRLETDLREIREQRNDRERELARWEGQ
                     NVVNLAQAQDELNMVEREITILEGRARAIATASRLIEEAYSVFSHQHRVAIQHGVNSI
                     MSKWTGLDEREFLVDENFVVRFRVPSLGGNGNNQWKCEHLSQGAQDQLALAIRWAVLD
                     RLAGDVVLPLLLDDCFHMWDAQRRDSLRSFLQEHRQRQIILVTHDERFLDWGEPVRRV
                     SSEI"
     gene            complement(4727..5920)
                     /locus_tag="PKI05_00335"
     CDS             complement(4727..5920)
                     /locus_tag="PKI05_00335"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOA50669.1"
                     /translation="MRDTILHLADLHLDASPEARLDDAVRAWLLGARRRILNALASWI
                     ARDESRIGLVLIAGDLFDRHDPPQEVVSEARTALAEIARLVPVVTVPGNHDEYSYAQC
                     VYRRGDWPGVLVTSPEPQLVWKGQLGDSVCAVVSAAYQAGKVSPGQRLRFPSRSQVMG
                     GDDSNGVLIGLFHSTLADHFPDEFVRNERCFVLSHQEAANAGYDYLALGHFHTRREWR
                     IGACLAHYPGPPLGARIADPGSGQLSLVRIRNGTVTLEGLSAGPMVGCQWMFLDVELQ
                     PEEKPREVVERIADRCRNVEANSGIAAIHVARLKGNTIHDNLAEQIQNELRAKGVSCT
                     VVAHELEEILSPDIEAMAAEESLVGYFVRSWQEWQSRQEVSRAEAMAVLYEGLLALGW
                     HRANN"
     gene            6567..7505
                     /locus_tag="PKI05_00340"
     CDS             6567..7505
                     /locus_tag="PKI05_00340"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOA50670.1"
                     /translation="MLHAVSRRDFLYYGAATAGTMALGNRFGWALENAAPKMVITRWT
                     GPMPQSPSEFQQAVVKLTEQTVQAMGGISRFVKRGDVVWIKPNIGWDRTPELAANTNP
                     DLVATLVRLCYEAGAKRVKVGDNPCDLPAKSYASSGIADAAKKAGAEVIFLDRERFRE
                     TDIKGERVKSIPVYPEIVESDVVINVPIAKHHVLATVTLCMKNYMGVIENRRLFHQDI
                     PTCLADITRFMRPTFCILDCTRIMVNHGPKGGNLADVQVKLTVAAGTDIVALDAFGAE
                     ILGRQPSQIGSIVKGQQAGLGTMDYRSLQPKEIALS"
     gene            7502..9142
                     /locus_tag="PKI05_00345"
     CDS             7502..9142
                     /locus_tag="PKI05_00345"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOA50671.1"
                     /translation="MKRRIAPRRWVRRVVQLVFLTLFVGLVLATRPVPGQLPSDWLKV
                     FFFFDPLILVLTGIAAWGIPTLALLGIVTLVVTALLGRVFCGWICPLGTVHAMTGWAL
                     DKVQKAKPGRDIWSPWQKTKYYVLIAALVMALFGGHWITIFDPLVLFYRTLTTALIPS
                     GQWAIESGATAIYKANPRIGSFAVTDLSEPVYRYFRDHVFVIPKQAFLGTGPITLFFV
                     VIVAANAYRRRFWCRYLCPLGALLGWAAKWPILRRAVTADQCNECGLCTTACHGASAV
                     GQGTGWKPSECFVCLNCSTSCRRDACAFTLQPLVWKKEPPLNPVGLTRRGVFWAGLGG
                     LTSLALFRATPQGRASIYHPRLIQPPGARPEPEFLARCTACGMCMKVCPTGGLQPAIT
                     EAGLEGIFTPHLVPLIGYCDYTCTLCGHVCPTEAIRPLLEEEKHQIKIGLAAFDTTRC
                     IPYVYGRNCVVCEEHCPIPDKAIYCVPVEIELRDGKKRVIQQPRVDPEKCIGCGVCEY
                     VCPLDDAPGIRVMSTNETRHVRHQPILPESDAEFDLPY"
     gene            complement(9315..10262)
                     /gene="rbsK"
                     /locus_tag="PKI05_00350"
     CDS             complement(9315..10262)
                     /gene="rbsK"
                     /locus_tag="PKI05_00350"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOA50672.1"
                     /translation="MTTMNDARVIVLGSINTDLVVKVPELPRPGSTVIGGTFYQSQGG
                     KGANQAVAAARVGRSVVTLIAAVGDDGFGRESLAHLGQEKIDLEYVVTKPQSPSGVAL
                     IIVDQHAENMIAVASGANALLSPSDVAAVPETVFRSAKVFLTCLEIPVETVIAGLKRA
                     KEAGLITILNPAPALDRLPPDMLNFIDIITPNQSEVSRLAGVEVSDIDSAQRAAYVLR
                     QKGVNKVIVTLRSQGCLVVDAERTAAYPAFRVNAVDTTGAGDAFNGALAAALAENQPW
                     EEAIRWATCAAAISVTRPGAQTSFPTRDEVEQMLTKAGS"
     gene            complement(10354..>10876)
                     /locus_tag="PKI05_00355"
     CDS             complement(10354..>10876)
                     /locus_tag="PKI05_00355"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50673.1"
                     /translation="GSNSIVDQAAQAKARALIERADIRLFCVDVSRPLQSDEQTWLEH
                     LDPASIVVLTKIDLEPDEATIRSLPDCLRVSAQTGVGVNELKDLIIARLIEHHQSTAD
                     IVTATAVRCRSALLRAHEALTEAEALARSNSPEETIASALRAAIDEFEIILGTVYTED
                     VLDRIFSRFCIGK"
BASE COUNT         2265 a         3306 c         2923 g         2382 t
ORIGIN      
        1 acctttaacg atccgtgagc tattcatgcg gggcgacatg ctgtcgtagg cgtgaatgtc
       61 ctggccgtga aacacgatgg tgcatcaatc gccgatatat cgcacgccag ggctcacgtt
      121 cctgcaaatg tggcgcaaca ctccgggcgc cattttgcgc actacctcgg gcgggccttc
      181 agcttgggag ggacgccgtc cccggcgtcc gtcatttctc cagggagcgt ttagattaac
      241 ttcggccgtt tgggagggac gccgtccccg gcgtccgccg tattttcact cggcgggtgt
      301 ttgccttgcc ctcggccacg acggattgcg gcccctgtct cgttatcccc cgcggagcgc
      361 gtttgatcgc cttaaaggga acctccctcc tggcgtaccg cttgtttgtc atgagcgtgt
      421 caccatcggc tggttccgct ctcgcgcgat gctcgcacct aaggcttaat caggcagacc
      481 catgtttgac ccctcccaag ggcacttcct aggacgacgc aatcatgtgg ctcctgccgg
      541 cctcggatgt tcgcctgacc gcatgaagcc aaatcatcca gtgcgagcta cgagcttatc
      601 ccacaaccac cccttgcccg ttcggcggga gaggggccgg gggtgagggt ggcttgaatc
      661 gctaccgggt ttctcgatcg actccgacag tcccttgccc tcaccctaac ctcctcctag
      721 agggcgtggg aactttggga taggctctaa cccatagaat ctcacggcgt tatcgtggaa
      781 gagttttcgg cgttcttcca gggatcggct tgccgtgatc tcacgtaagg cggcaatcca
      841 cgcccggaga ggcgcacctt ttgtgcacac tggccaatct ccggcgaaca tgacacggtc
      901 agagccgaag gcctccaaac agtggttgac gatcggggca agatcgtcgg gacgccaatc
      961 gtctggtctt acgcgcgcga tgatccccga gattttgcag acgacatttt ttcgcgctgc
     1021 caggccgtcc attgcccgcc gccactgctc tacataccga gtggcctctt cgccgcgctc
     1081 ctttccccag cgaccaaatt gctgggggtc agcgttgcca caatgatcga taataaaccg
     1141 tgtgtcagga caggcatcta ccaactctgc cgcgtcgctc agccagtcgg gcgggatgca
     1201 gatgtcgaaa ctcattcccc attcacccaa tagtcgcaag ttgtcccgca tctgtgggcc
     1261 gaaataaagc ttgtgtccca acttactatt gtgtggaatc cagcgaacac ctttgaccac
     1321 cgggttattg cgaaagcgag caagataggt ccggaaatcg tcgctggcgg gacgccctcc
     1381 aatgacggca gccaccgtcg gtccccgccc actacggcaa atctcaatga tccattcagc
     1441 ttctttgaca aggtcctcat cggccacggc cacttccata tagacggcct ggaccaccgg
     1501 caagcccttc acggcttcca gatagtcttc cacaagatag ttccgattga gtcgctcgtc
     1561 accggccaac cagggaaggc gaatttttga caagtcccac aaatgctgat gcgtatcgac
     1621 gatcgggatt gttgtcagct ccgggaccgt ctccgaggcc tccaagccca ggctaccacg
     1681 ggaaaaccga ttctcaagac caccggcaag ataccccagc agtgccgcgc cttgttgtaa
     1741 gaaggaacgc cgtgtgcagg tcatgatcat cttcctttct cggttagcag cctatccccc
     1801 aacctccccg tgcccgttcg gcaggagagg ggccggggtg tgactccgac gatcccttac
     1861 cctcgcccca actctctccc aaagggagag ggaactttgg gatacgctct aagacaagcg
     1921 actaggccca tcctgtatga ataaagtata acgattcgcg aaacgatgtg gagaaatcac
     1981 gtccagccga catcacgggc cgtcagtgca agctggcggg tcgtcatacg ttgctggcag
     2041 ttggaaaacc tgcggccaat aaaaagcaac tcattctaca gcgggagccc gccacattga
     2101 aacccgagac gaatttcgat tatcaagggc gctcgtcata tttccgagga cacgcgccgg
     2161 acaggttctc cccaatcaag gaaacgctca tcgtgggtaa ccaggataat ctgccgctgg
     2221 cgatgttcct gtaagaagct tcgcagactg tcgcgtcgct gggcgtccca catgtgaaag
     2281 cagtcatcca aaagaagtgg cagcaccaca tccccagcca agcgatcgag caccgcccaa
     2341 cggatggcca gggcaagttg gtcctgggcc ccttggctca ggtgctcaca cttccattga
     2401 ttgttcccat tgccacccaa gctgggaacg cggaatctga cgacaaagtt ttcatccacc
     2461 aaaaactctc gttcatccaa ccccgtccat ttgctcataa tgctattgac gccgtgttgg
     2521 atggccacgc ggtgctggtg cgaaaacacc gagtaagcct cctcgataag acgcgaggcc
     2581 gtggcaatcg cgcgggcccg cccctcgaga atagttattt cccgttcaac catattgagt
     2641 tcgtcttgag cctgggcaag attcaccaca ttttgcccct cccaacgcgc caactccctc
     2701 tcacgatcat ttcgctgctc gcgaatctct ctaagatcgg tctccagccg ggtaagctcc
     2761 tcctcgagac gtcgatattc gttttctaat cttcgagcat cccaggagaa tgcctcttct
     2821 gcgagggggc tgtcctgcgc cagttttcgc gactccagga tgatcccctg taggtccccc
     2881 tccgcctggc gatgtcgctc ttgtaacgac tcaatggtgt ccacgccaac gctctggagg
     2941 acaatctttt gctgttgccg ataagactgc tcgtgggcca ccagcttttc gtattcactc
     3001 aaaagactga ggcccctttc gaacacgtcc gggccaagtg gggctgaatc cgccgcctcg
     3061 ctttcgagac cacacatgtc tgtcaattct ttggggatgg ccctaaccgt aagttgggcg
     3121 gccacctgct ctagctccgc cttttggttg agaagctgcc tcttggcatc acacaggctt
     3181 ttaaagcgtc ccaaccgttc caacaaaatg ggcaattccc gcgtttgtgc ccagcgggct
     3241 atttcctccc aatatttctc caaggcccga acgcattttt catgatcccc ttcggcttgg
     3301 gccacctttt gctgccattg ggcatactcc ggtggccaac actccggtcg ataaatctgc
     3361 gccgttatca agcctcccgc aatggccgcg ataactccca ccagtaggcc catgaccagg
     3421 tagccaaaca gaccgaggat caaaacgagg accagcatca ctgccgcagc aacaccaatg
     3481 acgacgggca tctgccgttt acggtccagg acgggtcgga tacttgccgc gcgggacacc
     3541 gcctcgcgat ggtgttgctc ggcctcgtgg cacagcgcta aggccttctg aatctgctcc
     3601 acgcgcgaag gggcatccgc gggccaatcc cagacgtcgg caaaacgttc tttgaggacc
     3661 tcctcattca ccacgcgcga ttgttcgatg agccgtcgtg ccctctcttg atacttccgc
     3721 aaagcctctg cccagtactt tcgacgttcg cttgtgctgt cgcgaagaaa ggcaggaaac
     3781 ctctggagct gctctctcac ctcccgggct gaatcttcca attttttcca ctgatcaaga
     3841 gcacgcgcga ggtttcccgc caattcctct ttggtgcgac gttcagcaga aagccggcgc
     3901 gttttgtcca agattccctt gtttcgctgt aattcgttgc gcttttcttc cttggcggcc
     3961 acttgttcgt ccagccgctg gatttcctcg cgaaggcgga ggcctttttc cagcatctcc
     4021 ttggccaatt cacagtcacg ctgcaactcg tctcgtcgtc gccgtaactc atcgagccga
     4081 ccgtccttgc gggcatccga agtacttaat ccttctttct tggaataccg cgtgattgcc
     4141 cgataccgct cgaggagtgt ttctaacgcc tcgtcctttg tgctctcgcc cgccccggag
     4201 attaaggact gcaccaattc gccctcaggc tgaggattga acggaggcga aagtacgaca
     4261 agccgctcaa aggcccgggg cgagatgggg gcaaatacct cttgcaccaa gttcatccac
     4321 tgttcactgt cggcagtgcg gccccgtcgt ttgtgacggg tgacctgcac ggattgccat
     4381 tctccctttt ccaggcggaa aagacaaacc cggcccgttc ggaagtcccg ctcaacccga
     4441 tagcggatcg gcggctctgt ttcatgacgc ggctgaagtg cagcgcgttc gtactcaata
     4501 gccgccgcga atgattcgtc ctctcccgcg aaccaattgc actcgggaac cccagaaccc
     4561 cataacaccg ctgccacaag ccgcaagagt gtcgttttgc cggtttcatt gggggcgcaa
     4621 aacacggcca cacctgtggc aggaaattct aagcggtggt gtccccgcca ggggccacaa
     4681 ttcgtgacat caattgccaa gagtgcgatt cgcgtcatgc ctgccctcaa ttgttcgccc
     4741 ggtgccaacc taaggccaaa agcccttcat agagcacggc catggcctcc gcccgtgata
     4801 cctcttgccg agattgccac tcctgccaag atcggacaaa ataaccgacc aaactttcct
     4861 ccgcggccat tgcctcgatg tcgggagaaa gtatttcctc gagttcgtgc gccacgacgg
     4921 tacaggaaac ccctttcgct cgcaattcat tctggatctg ctcggcaaga ttatcgtgga
     4981 tagtattccc tttaagtcgc gccacgtgga ttgcagcgat gccgctattg gcttccacgt
     5041 tgcggcaccg atcggcaatc cgctcgacga cttctcgcgg cttttcctcc ggctgaagtt
     5101 ccacatccaa gaacatccat tggcatccca ccatgggacc ggccgaaagg ccttccagcg
     5161 tgaccgtccc gtttctgatc cgcacaagcg acaattgtcc actgccggga tcggcgattc
     5221 gcgcaccgag gggcggcccc ggatagtggg ccaaacacgc accgatccgc cactcccgtc
     5281 gcgtgtgaaa gtgccccaaa gccaggtagt cgtaaccggc attggcagcc tcctgatggg
     5341 agagaacgaa acatcgctca ttcctaacaa actcatcagg aaaatgatcg gcgagtgtgc
     5401 tgtggaagag gccgatgaga acaccgtttg aatcgtcccc acccatcacc tgacttcgtg
     5461 acgggaaccg aagtcgctga ccaggcgaca ccttaccagc ctgatatgcc gctgaaacca
     5521 cagcgcacac cgagtccccg agttggcctt tccagacaag ctgtggttcc ggcgaggtca
     5581 caagcacacc gggccagtca ccccggcgat agacacattg cgcgtagcta tactcgtcgt
     5641 gattccctgg gacagtcacg acgggcacca gtcgagcaat ctcggccagt gccgtgcggg
     5701 cctcggagac cacctcttgc ggcgggtcat gccggtcaaa gagatcgccg gcaatcagca
     5761 ccaagccaat tcgcgattcg tctcgggcaa tccaactcgc tagagcattc agtatccttc
     5821 gccttgcccc gagcagccag gcacgcaccg cgtcgtccag ccgggcctct ggggatgcat
     5881 ccaagtgaag atcggcaaga tggagaattg tgtcgcgcat tgccccattt tcctaacaga
     5941 aaaaacacac cgtcaagcgg cacgcaatca ccttttagcc cggtctgccc ctaagttcgg
     6001 atgtgtgccc aacccaccat cgaaggcccg aatggttcga cagggaggca ggcttatttc
     6061 ggtttcttag gccgaggtac cacctgagca caccgtttac accgcgtccg ctgggtgttc
     6121 aattttgccc cacattttgg gcaacgacga ttgtgcaaaa tgtggtagaa cttggtcctt
     6181 ggtcgtgttc gcatagtaag atcgacgttc ttttccgtag agcctaacaa gattccgtct
     6241 tgggaacatc cgccattcag cacattggct tgactcggaa cacattcaat gtaaaaggtg
     6301 tcccgaaatg cgccagtgcc cttcggtccg gcgaatcgac ggtcgtacat aggcgcgtcg
     6361 cagatcacta cccgcataca gcagcctgtg gaggtgtcaa acaacgtcgc tttttttgtc
     6421 ataaggaatt gtcattgtga gtcgaatgaa ataaagtagt aagacgtgat gtcggtcatt
     6481 gctaacaact agccaaccgg gcggaagtgg ccacggtcta ttttgcccac ctgttacggt
     6541 ctcacttgcc agaaaggatg acacccatgt tgcacgcagt atcacgtcgt gactttttgt
     6601 attatggagc agccacagcc ggcacgatgg ctttgggaaa tcgtttcggt tgggcactgg
     6661 aaaacgctgc accgaaaatg gttatcaccc gctggaccgg gcctatgccg cagtccccgt
     6721 ccgagtttca acaggccgtc gtcaagctga ccgagcaaac ggtccaggca atgggtggta
     6781 tttcacgctt tgtcaaacgg ggtgacgtcg tgtggatcaa gccgaacatc ggctgggacc
     6841 gcacacccga gcttgccgcc aataccaacc cggacttagt ggcgacgctg gttcgcctgt
     6901 gctacgaggc cggcgcaaag agggtcaagg tcggcgataa cccgtgcgat ctacccgcca
     6961 agtcatacgc cagtagtggg atcgccgatg ccgccaagaa agcaggagcc gaggtcatct
     7021 ttctcgaccg tgaacggttc cgcgaaaccg acatcaaggg tgaacgggtt aagagtatcc
     7081 ctgtttatcc ggaaatcgtg gaaagtgatg tcgttattaa cgtccccatt gccaaacacc
     7141 acgtgctggc gaccgtcaca ttgtgcatga aaaactacat gggcgtgatt gaaaatcgcc
     7201 gtctcttcca tcaagacatc cccacatgtt tggccgatat tacccggttt atgcggccga
     7261 ccttctgcat cctggattgc acccggatta tggtgaacca cggaccaaag ggtggcaatt
     7321 tggcggatgt gcaagtcaag ctgacggtgg cggcagggac cgacatcgtg gcccttgatg
     7381 ccttcggcgc ggagattctt ggccgccagc caagtcaaat cggctcaatt gttaagggtc
     7441 aacaggctgg attaggcacc atggactacc gctcgctaca gcccaaggag atcgcccttt
     7501 catgaaacgt cggatcgccc ctcgacgctg ggtgcggcgg gtcgtccagt tggtgtttct
     7561 gactctcttt gtcggcctgg ttctggcgac gcggccggtt ccggggcagt tgcccagcga
     7621 ttggttaaag gtgtttttct tttttgaccc attgatcctg gtcctcaccg ggattgcggc
     7681 ttggggtatt cccacgttgg ccttgctggg gatcgtcact ctggtggtaa cagccctcct
     7741 gggccgcgtc ttttgtggtt ggatttgccc cttggggacc gtccatgcta tgacgggttg
     7801 ggcgctggac aaggttcaaa aggccaaacc cggccgcgac atttggtccc catggcaaaa
     7861 gaccaaatac tatgtgctca tcgcggcctt ggtgatggcc ttgttcggcg gacattggat
     7921 cacgatcttc gatcctcttg tcctctttta tcggaccctc accacagcgt tgattcccag
     7981 cggacaatgg gcaatcgaaa gcggggcaac ggccatctac aaggccaatc cacggatcgg
     8041 ctcctttgcc gtaacggatc tcagtgagcc cgtttaccgc tactttcggg atcatgtatt
     8101 cgtgatccca aaacaggcat tcctcggcac gggaccgatt accctgttct ttgtggtgat
     8161 tgtggcggcc aacgcatatc gacgccgctt ttggtgtcgg tatctgtgtc cgcttggggc
     8221 actcctgggc tgggccgcca agtggcccat ccttcgcaga gcggtaacgg cggaccaatg
     8281 taatgagtgc ggactctgca ccaccgcctg ccatggggca tcagccgtag gacagggaac
     8341 gggctggaag ccgagtgaat gcttcgtctg tctcaattgt tccaccagtt gtcgccgcga
     8401 tgcctgcgcc tttaccttgc agccgctcgt ctggaagaag gagccgcctc tcaatccggt
     8461 tgggttgact cgccgcggcg ttttttgggc tggtcttggg ggactaacga gccttgccct
     8521 ctttcgggcg acaccgcagg gacgggcctc gatctatcat ccgcgtctta tccaaccgcc
     8581 gggagcacgg cccgaaccgg agttcctcgc ccgatgcacg gcttgcggta tgtgcatgaa
     8641 ggtctgtccc acaggcgggc tccaaccggc aatcaccgag gcgggcctcg aggggatctt
     8701 tacaccccat cttgtacccc tcatcggcta ctgtgactat acctgcacct tgtgtgggca
     8761 tgtctgtccg acggaggcaa tccggcccct actggaggaa gagaaacatc aaatcaaaat
     8821 tggcctggcc gccttcgaca cgacccgctg catcccctat gtttacggcc gaaattgcgt
     8881 ggtctgtgaa gagcactgcc ccattccgga caaggccatt tattgcgtgc cggttgaaat
     8941 cgagctgcgc gatggcaaaa aacgagtcat ccagcagccc cgcgtggatc ccgaaaagtg
     9001 tattggctgc ggcgtgtgcg aatacgtgtg ccccctggac gatgccccag gaattcgggt
     9061 catgagtacg aacgagacac gacacgttcg ccatcagccg atccttccgg aaagtgacgc
     9121 cgagttcgac ttgccttact aagagcctat cccaaagttc cctcttcctc tgggagaggg
     9181 tagggatgag ggcaagcgat cgtcggagtc gatcgaaaaa actggcagcg attcgagcca
     9241 ccctcaccgc cgatcgctct cccgccgaac gggcgtggga agatcatggc gtaggctgta
     9301 agcctgagta ccggtcaact gccggccttc gtcaacattt gttccacttc atccctagtg
     9361 gggaaggaag tttgtgcccc tgggcgggtc acggaaatgg ccgccgcaca ggtggcccaa
     9421 cgaattgcct cttcccacgg ttgattctcc gccagtgccg cggcgagagc cccgttgaaa
     9481 gcgtcaccgg cgccggtggt gtccacggca ttgacgcgga aagcgggata tgctgcggtt
     9541 cgctccgcat ccaccacaag acacccttga cttctcagtg tcacgatcac cttgttgacg
     9601 cccttctgcc ttaggacata ggccgcccgt tgtgccgaat cgatgtcgga gacctcaact
     9661 cctgccaatc gggagacctc cgactggttg ggagtgataa tgtcaatgaa attgagcata
     9721 tcagggggaa gcctgtccaa ggccggggcg ggattgagga tcgtgattag tccggcttcc
     9781 ttagcccgct tcaaaccggc aatcacagtc tccacgggaa tctctaggca ggtcaaaaaa
     9841 accttagcgg agcggaatac tgtctcgggg accgccgcaa catcggaagg cgacaagagg
     9901 gcatttgccc cggaagccac ggcaatcatg ttttcggcgt gctgatcgac gataataagt
     9961 gcgacacccg atgggctttg gggttttgtg accacgtact caaggtcgat cttctcttga
    10021 ccgaggtggg cgagactctc ccgcccaaat ccgtcgtccc ctacagccgc gattaaggta
    10081 acgacggagc gtcccacacg tgccgcggcg accgcctgat tagccccttt tccgccctgg
    10141 ctttgataga acgttccccc aatgaccgtg ctccctggcc gagggagctc cggaaccttc
    10201 acgaccaaat cggtgttaat ggagccaaga acaatcacac gcgcgtcatt catcgttgtc
    10261 atgtatggct aaaaagcttt tttcgaacga acattgtcgc agacccaaat cccctcggat
    10321 ctcccgtctg ccgcgattgc cgcgtcggag atatcacttg ccgatgcaga accgcgaaaa
    10381 aatgcggtct aacacatcct cggtgtaaac cgtccccaag atgatctcga attcatcaat
    10441 cgcggcacgc aaggcactgg caatagtttc ttccggggag ttggatcgtg caagggcctc
    10501 tgcctcggta agggcctcgt gggccctcaa cagtgctgag cggcaacgaa cggccgtcgc
    10561 cgtcacaata tcagccgtgg attggtgatg ctcgataagt cgggcgataa tcaggtcctt
    10621 cagctcgttg acgccgacgc ccgtttgcgc actgaccctg aggcagtctg gcaagctccg
    10681 aatcgtggct tcatcaggtt caagatcaat cttggtcaaa accacaattg aggccggatc
    10741 gagatgttcc agccacgtct gttcgtctga ctgaaggggc cgagacacat cgacgcaaaa
    10801 aagccgaata tccgctcttt cgatcaatgc tcgcgccttg gcctgggctg cctgatccac
    10861 tattgagttc gagcct
//
LOCUS       DAOPTR010000009        10498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_5321_length_10498_cov_3.672221, whole genome shotgun sequence.
ACCESSION   DAOPTR010000009 DAOPTR010000000
VERSION     DAOPTR010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 10498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10498
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5321_length_10498_cov_3.672221"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="PKI05_00360"
     CDS             <1..1225
                     /locus_tag="PKI05_00360"
                     /inference="COORDINATES: protein motif:HMM:NF016991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HOA50674.1"
                     /translation="FQTETGLDHIRSECRQLAVENEFAINGHENRISYVVGTGHRYQV
                     MPRPGCRVPRSLLAQAEAVLDRFLRENRWHARQQEIMRRKDRDGEAFLRFFPGDDGIL
                     RVRFINPEDIRTPTQYSQRPEHSFGIATDPSDVETVHGYWVCEQWVPADQMQHRKANV
                     DANVKRGLPLFYPVRKNLRRAEKLLRNMSTVAGLQSAVALIRKHTGGRSSVERFLHDA
                     APPVPGEPSASTGYTSVQRFAPGTILDATAGVEYEFPVAAIDAARYVTLLQAELRAVA
                     SRLVMPEFMLTSDASNANYASTLVAEGPAVKMFQRLQAEMIEEDHVVLERALQTAAHA
                     GLLPVDVLQQVTIKAVPPSLVVRDRLREAQADAILFRCGALSRETLALRHSLEPNTTN
                     DSPMDESIRGEQRNP"
     gene            1278..2153
                     /locus_tag="PKI05_00365"
     CDS             1278..2153
                     /locus_tag="PKI05_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50675.1"
                     /translation="MSHTHICEYLGWGDCPMKVDAHNGVIYGVKVLGLISRNGRRYSE
                     QALRQALPLYEGARVNINHPRGDAAAPRDYRDRLGFLRTVRYRDGEGLFADLHFNPKH
                     DLAERLAWDAEHAPHNVGLSHNVLARVEHQGQIPVVTEIHRVLSVDLVADPATTSGLF
                     EAKSPASSESTQAAAHGDGGEPPEMLELARLRGELALRRHIREWHLLPPDETASEGEQ
                     KMGPRLWEILISETDDSRRRQLLAELANLARPQTSHQTWQSSRPTSRDPLGVCECPNT
                     AAFVAAITGRGASWK"
     gene            2340..2819
                     /locus_tag="PKI05_00370"
     CDS             2340..2819
                     /locus_tag="PKI05_00370"
                     /inference="COORDINATES: protein motif:HMM:NF021473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HOA50676.1"
                     /translation="MAHVMRWRYGDTQPVVATVDADSNIELGDLLFWDGDDAKPASLQ
                     PDQGTEAANQQLFASKFLGVAMQASPAGESSPIRVATAGVFEFTTPGGSFDLGDWVGV
                     DENDAGTVLENQRLVKVTSSQLAIGRVARRSMDGHTVWVAICSTVMAGGVKGTTATT"
     gene            3038..4210
                     /locus_tag="PKI05_00375"
     CDS             3038..4210
                     /locus_tag="PKI05_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50677.1"
                     /translation="MPAISYRELRRRYELDGAEKTIHHLSEALEKKHLRPDDFSLRDL
                     AESLIPEGRQWVKALDPRQSSPVSIMEAGDAVDVTAFLNVTGQIIYAQILETYRHEAF
                     VASRLVRTIPTRLDGEKIPGTSGIPETMTEVLPGMPYPHVGFSERYVETPSTTKHGLI
                     VAVTREAIFFDRTHIVLRQAAEVGETLGRLKEKRILDVIIGAVNTYRENGVAYNTYYA
                     AGSGGPWVNVLANNPLEDWTSVDRAEQLFSEMTDPLTGEPILIRPNAVLVPSALRHTA
                     RRVFHATAISFAADGSVTATTFANPLSDYRVWDSQLLYQRIVASGVSAEVAKTWWLIG
                     DFARAFAYMENWPLTVTQSAPGSEADFTQDIVVRFKASERGTAAVLDPRYVVRCSG"
     gene            4264..5076
                     /locus_tag="PKI05_00380"
     CDS             4264..5076
                     /locus_tag="PKI05_00380"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOA50678.1"
                     /translation="MARLFNGTSQFLRRVDGTGLTLPLTLAAWVYPQRNTAYEEVLAL
                     ARSSDNSTGWFLQIRGPDGMRVAAVTASQNTFAIARSTGSYALNQWQHVAGVFSESNA
                     RRVYLNGVPGALDTTVLNVASVDRMLIGAWERLGGWLAYFGGAVAEAAIWQGALGDSD
                     LAQLAAGYSPLLVRSQDLIAYWPLGGPFGNNDQDHSGNGFDLTAYGDPTWADHPPIVY
                     PQPPEAPPAEAFLGVTGSVITAGAVAGMGFHAGNQAGSVHARLALGQVRIPR"
     gene            5107..5499
                     /locus_tag="PKI05_00385"
     CDS             5107..5499
                     /locus_tag="PKI05_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50679.1"
                     /translation="MSAAEIYGVVFQNATAAFLARVLDYDGQPVTPSQIASVEYTVAE
                     HLDDMTETTPIDGHTAVALNPSDVLFATLQKGGLWDVDDVGYNFRHVIDVSQAPAFPT
                     AGSHYRITYRLTPQTGQVIVVRFRVKAI"
     gene            5551..5766
                     /locus_tag="PKI05_00390"
     CDS             5551..5766
                     /locus_tag="PKI05_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50680.1"
                     /translation="MADDRDVIQLIRSQTLELIQQITAQPKPSYELDGQRVSWTEYLG
                     RLIEVVNWCDRQLAAETPCEIRSLGLT"
     gene            5778..6419
                     /locus_tag="PKI05_00395"
     CDS             5778..6419
                     /locus_tag="PKI05_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50681.1"
                     /translation="MRTDLIADDLSQFADRLETITHIRRGQNRGMTVQGAFRRAVPPR
                     REDRDDGWSGADMVWHLPEQAVPGGVRPGDHLVDGAQRRWTVLVVSPTASGRIIAWTR
                     DWSAAFSTGRTVDVERAEITQGASGEAIPRWTTAAAGVPAEFVSCHCDRSETGTDRLP
                     VYRVLVLWDGPQGRYRLKTDDGQYYSVLKVERPALLGHPTVFYVSPAPANHPT"
     gene            6463..6867
                     /locus_tag="PKI05_00400"
     CDS             6463..6867
                     /locus_tag="PKI05_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50682.1"
                     /translation="MITLEQALRTRWQDDPTLAALLPVNYVWTNWSDVAQVPRAEITA
                     AGEDRVWLTSRAEVAERVRVTVEVWHNSFEKLRSTINRIKELFDRASFDLGDNARILR
                     LTYQRDDMARQGYGLWRGEITFDGLALRPIVA"
     gene            6924..7433
                     /locus_tag="PKI05_00405"
     CDS             6924..7433
                     /locus_tag="PKI05_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50683.1"
                     /translation="MTTVLRSTLLAKLGWTWQDEQDRAVVADSNQWTWRTALNEGTQP
                     GEANAIWYAVGKTLASGASTEWLLDALSRPLFGKTIVQGFSSVKVLWLANRGESTGTL
                     VLGGASSNPWPGPLSSGSSTLCVAPGGVLFAVHPAAGWGVSSSAKAIKLLAEAGSVTY
                     DIILLGVAS"
     gene            7536..8384
                     /locus_tag="PKI05_00410"
     CDS             7536..8384
                     /locus_tag="PKI05_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50684.1"
                     /translation="MPVTFRELAGSPEETYTLEGFRARRTFLVPWEERHSFAAEILGS
                     GGDHGGRPWSRYPGKESVFAVAIRFSPADPEALRTLTEESPDVQEVLAEYSGSFAQAI
                     VEYTTITPEEREDTPSPPLQTHLSYRMEYGLMERPLLASGFFAEDDPQTPLAAELPLT
                     HIIPYTDHYLVWQQVINPPWQAIRELQGTVNEEVFLNATPGTLLFLGATTNKLFRGSF
                     DEGVSPFCWEIRYHFRELAIKHGGTVYGWNHLHRDQPAGYVAIADPEGPLYDSGDFSR
                     LFLPEV"
     gene            complement(8723..9925)
                     /locus_tag="PKI05_00415"
     CDS             complement(8723..9925)
                     /locus_tag="PKI05_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50685.1"
                     /translation="MNDSPQAEFRKPLFWILAGVIVSVLAIVLAVYPWPAPEVDVTYG
                     TMGWLGRKSINGTWVLAEMVRRSGHSVSRSTHLSPMLKEQADCLIWFAADPNVPHESV
                     VTWLEDWLKAKPGRTLIYVGRHFEATSLYWKKLLERFPDQYDPVRQKEIARRQALEEQ
                     FLQLISNVEGQCAWFSFKPLPDVVRPKSLEMHPQWRVPVNPQDLEIELRSQMIPAQDA
                     EVLIRSEKHVLVARLPRGSSQILLVNNGSFLLNMMLVNREHRKLARRLVQAIDESGTS
                     GPKRVCFLELRPGYQVVTGPTEEPSPYRFLTVYPLNVIVLHLFLLAVLFALARFPRLG
                     PGYEPDLVLRTSFDAHLAAMARWLRRTRDYQFVQEVISQWERISAPRGQGGRGLRERP
                     HKSRLLRY"
     gene            complement(9922..>10498)
                     /locus_tag="PKI05_00420"
     CDS             complement(9922..>10498)
                     /locus_tag="PKI05_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HOA50686.1"
                     /translation="YDAEKDDIKPTRVRKPRELPEPSKVSRSRVPWSAVGIILIVLAI
                     AAMVILGYWAWRRWAPCQGGKEEAARVAAQLRELPLVPEDSPQETDLLQASERAAAAG
                     DYRRAIIWLYAYQLAELDRHRLIRLMKGKTNRQYLRELGRESPVAPLLERTIVTFEEA
                     YFGGRSLSAEQYEACRRELPQFQQWLKEIAA"
BASE COUNT         2091 a         3272 c         3031 g         2104 t
ORIGIN      
        1 tttccagacg gagacgggac tggaccacat ccgcagcgaa tgccgtcaac tggccgtgga
       61 aaacgagttt gccattaacg gccacgaaaa ccgaatcagt tacgtggtgg gcaccggcca
      121 ccgctaccaa gtgatgccgc gacctgggtg tcgcgtgccg aggtccctcc tcgcccaagc
      181 agaggcggtc ctcgatcgat tcctccgcga aaaccgctgg cacgcacgac agcaggaaat
      241 tatgcgtcgc aaagaccgcg acggtgaggc atttctccgc tttttccccg gcgatgacgg
      301 gatcctgcgc gttcgtttca tcaatccgga ggatatccgc acgccgaccc aatacagtca
      361 gcggcctgag cacagcttcg gtattgctac tgatccaagt gacgtagaaa ccgttcacgg
      421 ttactgggtt tgcgaacaat gggtccccgc cgaccagatg caacatcgca aggccaacgt
      481 cgatgccaac gtcaaacgcg gtctgccgct gttttatccg gtgaggaaga accttcgccg
      541 ggcggagaaa ctgctgagaa acatgagcac cgtggccggg ctccagtcgg ccgttgccct
      601 gattcgcaaa catacgggcg gacgcagcag cgtggaacgc tttctccacg atgccgcccc
      661 gccggttccg ggcgagccgt ccgccagcac cggttacacc tcggtgcaac gttttgcccc
      721 gggaaccatt ctcgacgcca ccgcgggagt ggaatacgag tttcccgtgg ccgccatcga
      781 tgccgcccgc tatgtcacac ttctccaagc cgaattgcgg gcagtggcca gccgcctcgt
      841 catgccggag ttcatgctta ccagtgatgc cagcaacgcc aattacgcct ccaccctggt
      901 ggcggaaggt cccgccgtca agatgttcca acgacttcag gccgagatga tcgaggaaga
      961 tcacgtcgtt ctggagcggg ctttgcaaac ggcggcccac gccgggttgc tcccagtcga
     1021 tgtcctccag caggtcacca tcaaggctgt gccgccatcg ttagtggtcc gcgatcgcct
     1081 cagggaggcc caggccgatg ccatcctctt ccgctgtggt gccctctccc gcgaaaccct
     1141 cgccctccgt catagcttgg aacctaacac cacaaacgat tccccgatgg atgaaagcat
     1201 cagaggagaa caaaggaacc cgtaaaaacc gttgtcacag attttctctc gttttcgtgt
     1261 cttaaagagg tccaacgatg agccacactc acatttgcga gtatcttgga tggggcgact
     1321 gtcccatgaa ggtggatgcc cacaacgggg tcatttacgg ggtcaaggtc ttgggcctta
     1381 tctcccgcaa cggacgccgc tacagcgagc aagcccttcg ccaggcctta ccgctgtacg
     1441 aaggggccag ggtcaacatc aatcatccgc gtggcgacgc cgcagccccc cgcgattacc
     1501 gcgatcgctt gggatttctc cgtacggtgc ggtatcgaga tggagaaggc ctctttgccg
     1561 atctccactt caaccccaaa cacgacctgg ccgagcgact tgcttgggat gccgagcacg
     1621 ccccccacaa cgttgggcta tcccacaatg tccttgcccg agtagaacac caaggtcaaa
     1681 tcccggttgt cacggagatt catcgcgtct tgagcgtcga tctcgtggcc gaccccgcga
     1741 cgacctcagg ccttttcgag gccaagtccc ccgcgtcgtc cgagagcacc caagccgcgg
     1801 ctcatgggga cggcggtgag cctcccgaaa tgctggagtt ggcccgcttg cggggtgaat
     1861 tggcgctgcg ccgccacatc cgagagtggc atttgctccc gccggatgaa acggcctcgg
     1921 agggcgagca gaagatggga ccacggctct gggaaatcct catctcggaa acagatgact
     1981 cgcgacggcg gcagctcctg gccgagttgg ccaacctggc gcgtccgcag acgtcccatc
     2041 aaacgtggca gtctagccgc ccaacaagtc gagaccccct tggggtgtgc gaatgtccca
     2101 ataccgccgc ctttgtggca gcgatcaccg gccgaggtgc ctcatggaag taaatgggag
     2161 aaaagaccgg ccgagcatta ctcggcgcca aacgtaccga tcttcaacca ggtttgactc
     2221 tttacacgac tgcgaagccc ctctgcccgt ctctgccgga gatggcactt gaggaagcag
     2281 cgacgtggcc tgggccactg ttgaccttca acacgtttac cgagtgaaag gagattttta
     2341 tggctcatgt gatgcgatgg cgttacggcg acacgcaacc cgtggtcgct acggtggatg
     2401 cggacagcaa tatcgaattg ggcgatttgc tgttttggga cggcgacgac gccaaaccgg
     2461 cgtcgctcca gcccgaccag gggaccgagg cggccaatca gcagttgttc gcctccaagt
     2521 tcttgggcgt ggcgatgcag gccagtcccg ccggtgagag tagtcccatc cgggtggcca
     2581 ccgctggggt attcgagttc accacgcccg gcggttcgtt tgacctgggt gattgggttg
     2641 gcgtcgatga gaacgacgcc ggaacggtcc tcgaaaacca acgcctggtg aaagtcacca
     2701 gttcccaatt ggctatcggt cgagtcgcac gccgttcgat ggacggtcat acggtgtggg
     2761 tcgccatttg ctcgaccgtc atggctggtg gtgttaaagg cacaaccgca accacctaag
     2821 agcttatccc acaacctccc ctctcccgtt cggcgggagt agggcctggg gtgagggtgg
     2881 ctcgaatcgc tgccggtttc tcgatcgact ccgacggtcg cttgccctca ccccaaccct
     2941 ctcccagggg gagagggaaa tttgggatag gcttcaaacg tgtagcttct tggtatttga
     3001 ggatccaccc atttggttct ttgatcaggg gtaaatcatg cctgcgattt cctatcgtga
     3061 actgcgtcgg cgctacgaat tggacggtgc cgagaagacg atccatcatc tcagcgaggc
     3121 cttggagaag aagcatctcc ggcccgatga cttcagtctc cgtgatctgg ccgagtccct
     3181 cattcccgaa ggccgccagt gggtcaaggc cctcgatccc cggcagtcct cgcctgtctc
     3241 catcatggag gccggtgacg ccgtggacgt gacggcgttc ctcaacgtga cgggacagat
     3301 catctacgcc cagattttgg aaacctaccg ccatgaggcg tttgtggcct cccgcttggt
     3361 gcggaccatc cccacgcggc tggacggaga aaaaatcccc ggcacgtccg gcatcccgga
     3421 aacgatgacg gaagtccttc ccggcatgcc gtatcctcac gtcggcttca gcgagcgcta
     3481 cgtcgagacg ccctccacaa ccaagcacgg cctgatcgtg gcggtcaccc gggaagcgat
     3541 cttctttgat cggacccata tcgtcctccg ccaagccgca gaagtgggcg agacactcgg
     3601 tcgcctcaaa gaaaagcgga ttctcgatgt catcatcggt gctgtgaaca cctaccggga
     3661 aaacggtgtt gcgtacaaca cctattatgc cgcgggatcg ggcggcccct gggtcaacgt
     3721 tctcgccaac aatccgctgg aggactggac cagtgtcgat cgggccgaac agttgttttc
     3781 ggagatgacc gacccgttga ccggtgaacc cattctcatc cggccaaacg cggtgttggt
     3841 ccccagcgcc ctgcggcaca cagcccgacg ggtcttccac gccacggcga tctccttcgc
     3901 agcggacggt tcggtcacgg ccaccacgtt tgccaatccg ctgtcggatt accgcgtctg
     3961 ggatagccag ttgctctacc aacggatcgt ggcatcggga gtcagtgcgg aagtcgccaa
     4021 gacatggtgg ctgatcggtg attttgcccg ggcctttgcc tacatggaaa attggccgct
     4081 gacggtgacg caatcagcgc cgggaagcga ggccgatttt acccaggaca tcgtcgtccg
     4141 tttcaaggcc agcgagcggg ggaccgcggc agttctcgat cctcgttacg ttgtccgctg
     4201 tagcggataa gaaagtgaaa gcgttgatgc ccgatccacg ccaacacatt acgaggctac
     4261 cccatggcac ggttgttcaa tggaaccagt cagtttttgc ggcgggtcga tgggacgggc
     4321 ctgacgttgc cgctcaccct agccgcttgg gtctatcctc agcggaacac ggcctacgag
     4381 gaggtccttg cccttgcccg atccagcgat aacagcaccg gctggtttct gcaaatacgg
     4441 ggacccgacg ggatgcgtgt ggccgccgtc acggcctcgc agaacacctt cgccattgcc
     4501 cgcagcaccg gcagttacgc ccttaatcag tggcaacatg tggcgggggt gttttcggag
     4561 tcaaatgccc gacgtgtcta tctcaacggc gttcccggcg cgttggacac gactgtgctg
     4621 aacgtggcct ccgtggatcg catgctgatc ggcgcatggg aacgactggg tggatggtta
     4681 gcctattttg gcggtgccgt ggccgaggcc gccatctggc aaggggccct cggtgattcc
     4741 gacctcgccc aattggccgc aggttattcg cctctattgg ttcgttcgca agatttgatt
     4801 gcctactggc ctttgggcgg accctttggg aataacgacc aggatcactc gggcaatggc
     4861 ttcgacttga cggcctacgg tgatcccacc tgggctgacc atcccccaat cgtctatccc
     4921 cagccaccgg aagcgccacc ggcagaggcc ttcctcggtg tcaccgggtc cgtgatcacg
     4981 gcgggggctg tggctggtat gggctttcac gcggggaacc aagcgggatc ggttcacgcc
     5041 cgccttgccc tcggccaggt ccgcatccct cgttgatatc ttcttcacca gaaacaggag
     5101 ccaaacgtgt ccgccgctga aatttatggc gtcgtcttcc aaaatgccac ggcagcgttc
     5161 ctcgcccgcg ttttggacta cgatggccaa cccgtcacgc catctcaaat tgcgtctgtc
     5221 gagtacaccg tggctgagca ccttgatgac atgaccgaga cgacaccgat tgacggccac
     5281 accgccgtgg cgctcaaccc aagtgatgtc ctctttgcca ccttacagaa gggcggcctt
     5341 tgggacgtgg atgacgtggg gtacaacttc cgccacgtga ttgacgtctc gcaggccccg
     5401 gcctttccca cagcggggtc ccattaccga atcacatatc gcctgacacc acagacgggg
     5461 caggtcattg tcgtccgctt tcgggtcaaa gcgatctaga tccgacacac gtggtcaagt
     5521 cttcccagca tagcaaagga ggaactcccc atggctgatg atcgagatgt gatccagctc
     5581 atccgcagtc aaacgctgga gcttatccag cagattacgg cccagcccaa gccgagctac
     5641 gagcttgacg ggcagcgggt ctcttggacc gaatatttgg ggcggctcat cgaggtcgtc
     5701 aactggtgtg accgacaact tgctgccgag acgccttgtg aaatacgctc cttgggactt
     5761 acgtgaggac acacaccatg cgcacagatt tgattgccga cgacctttcc caatttgccg
     5821 atcgacttga aaccatcacc cacattcgcc gggggcaaaa ccgcggaatg accgtccagg
     5881 gagccttccg gcgggccgtc cccccacgcc gcgaggaccg cgatgacggt tggtcgggag
     5941 ccgacatggt ttggcacctc ccggagcaag ccgtgccggg tggcgtgcgc cccggtgatc
     6001 acctggtcga tggcgcccag cgccgttgga ccgtcctggt cgtctctccc accgccagtg
     6061 ggcggatcat cgcctggacc cgcgattggt cggcggcgtt ttccactggg cggactgttg
     6121 acgtagaacg cgccgagatc acacaaggcg ccagcggcga ggcaattccc cgctggacca
     6181 cggcggctgc gggcgtgccg gcggagttcg tttcctgtca ctgtgaccga tcggagaccg
     6241 gcaccgatcg cttgcctgtg tatcgggttc tggttctttg ggatggcccc cagggccgct
     6301 accgacttaa gaccgatgat ggtcagtact acagtgtctt gaaagtcgag cgtccggcac
     6361 tccttggcca tcccaccgtt ttctacgtct cgcccgcacc ggcaaaccat cccacgtaaa
     6421 tgccatgttg aaccgttttt ctcccaagga ggacctacga ccatgatcac cttggaacag
     6481 gccctgcgca cccgctggca agatgacccc accttggccg cgctgcttcc tgtcaactat
     6541 gtgtggacca attggtcgga tgtggcccag gtgccgcggg ccgaaatcac ggccgctggc
     6601 gaagatcggg tttggctcac ctctcgtgcg gaagtagccg aacgggtccg cgtcaccgtg
     6661 gaggtctggc ataactcgtt cgagaaactt cgctcgacca tcaatcggat caaagagcta
     6721 ttcgaccgcg catcgttcga cttgggcgac aatgcccgca tcttgcgact cacctatcag
     6781 cgggatgaca tggcccggca aggttatggg ctgtggcgag gggaaatcac ttttgacggt
     6841 ttggcccttc gccccattgt ggcctgatat gtgggaagaa cgccgagctt tcatctcgcg
     6901 acaccaataa tcttggagga actatgacaa ccgtcttgcg aagcacgctt ttggccaaac
     6961 tgggatggac ctggcaggat gagcaggatc gcgccgtggt ggccgacagc aatcaatgga
     7021 cttggcgaac cgcccttaac gagggaaccc aacctgggga ggcaaatgcc atttggtacg
     7081 ccgtgggcaa gaccttggct tccggggcaa gcaccgaatg gttgctcgac gccctctcgc
     7141 gacccctttt tggcaagacc atcgtccaag gcttctccag tgtgaaggtc ctgtggctgg
     7201 ccaatcgcgg tgagagcacc ggtacgctag tcctgggcgg ggcaagtagc aacccttggc
     7261 ccggtccgtt gagttcagga agcagcaccc tgtgcgtggc ccccggcggt gtcctcttcg
     7321 ctgtccatcc tgccgccggc tggggtgttt catcctcggc caaggcaatc aaacttctgg
     7381 ccgaggccgg aagtgtcacc tacgacatca ttcttctcgg tgtggccagc taggtccgcg
     7441 ccgatgcgag tgtcttccgc cgattgatat tcaaacacgg tggtcgtcga agattccttg
     7501 gtcattccct cgaggcccca tcctggagag cttccatgcc tgtgacgttc cgcgaacttg
     7561 ctggttcacc tgaagagacc tacaccctgg agggctttcg cgcacggcgg accttcctgg
     7621 tcccttggga agaacgtcat tcctttgcgg cggaaatcct tggcagcggt ggcgaccacg
     7681 gtgggcggcc ctggtcgcgc taccccggca aggagagtgt ctttgccgtg gccattcgat
     7741 tctcccccgc cgatcccgaa gcccttcgca ccctcacgga ggaaagcccc gatgtgcagg
     7801 aagtgctggc cgagtactcc ggctcctttg cccaggccat cgtggaatac actaccatca
     7861 cgcctgaaga gcgtgaggat acgcccagcc caccgctgca aacgcatctc tcctatcgga
     7921 tggaatatgg gctgatggaa cggcctttat tggcgtcggg cttcttcgcc gaagatgatc
     7981 cccaaacacc tctggccgcc gaacttcctc tgacgcacat catcccctac accgatcact
     8041 atttggtgtg gcagcaagtg attaatcctc cctggcaagc catccgcgaa ttacagggga
     8101 cggtcaacga ggaggtcttc ctcaatgcca ccccggggac gctcctcttc ctcggggcta
     8161 ccaccaataa gctgttccgc ggaagcttcg acgagggcgt ttcccccttc tgctgggaaa
     8221 tccgatatca ctttcgagag ctggccatca agcacggtgg caccgtctat gggtggaacc
     8281 acctccatcg agatcagccg gctggatacg tggccattgc cgatcccgaa ggcccgctct
     8341 acgacagcgg cgacttctcc cgcttgtttc ttcccgaagt gtgaattgcc cgagccaaca
     8401 tgatgatcca cactgcggca tataagccat ggtttgcccc acgtttgtcc ggcacgtatc
     8461 ccagtgatgg cactaagagc ctatcccata atcttccctc atgcgttcgg caggagaggg
     8521 gtcggaggta agcgtgggtc gaattgctgc cggtttcctc gatcgattcc gacgttcgct
     8581 tgccctcacc ccaaccctct cccggaggga gagggggact ttttgggata ggcgcttaac
     8641 gccggtgagg gttccgagct gcctgccgcg tggatcgttg atcagagttc acccgtttgc
     8701 ctgtcgccgt atcttcagcg gctcaatacc gcaggaggcg tgacttgtgt ggtctttccc
     8761 gcagacctcg cccaccttgg ccacggggcg cactgattcg ctcccattgg ctgatcacct
     8821 cctgcacgaa ctgatagtcc cgggtacgtc gcaaccaacg ggccatcgcc gccagatgag
     8881 catcgaagga cgtccgcaag accagatcgg gctcatagcc ggggcccagt cgcgggaagc
     8941 gggccagggc aaaaaggacg gccaagagaa acagatgcaa aacgatcaca ttcagcggat
     9001 acaccgtcag aaaccggtag ggtgaaggtt cctccgtcgg accggtcacc acctgatagc
     9061 cggggcgcag ttcgagaaag caaacccgtt tcggcccaga ggtgcccgat tcatcaatgg
     9121 cctggaccaa ccgccttgca agtttgcgat gctctcgatt caccaacatc atgttaagga
     9181 gaaacgaccc gttattgacg agaaggatct gggatgagcc tcgtggcaaa cgggcaacca
     9241 gcacatgttt ttccgagcgg atcaggacct cggcatcttg ggccggaatc atctgcgagc
     9301 ggagttcgat ttccaagtcc tgcggattga ccggaacccg ccattgaggg tgcatctcca
     9361 agctcttggg acgaacgaca tccgggaggg gtttgaacga aaaccacgcg cactggccct
     9421 cgacattcga gatgagttgc aagaattgtt cctccagagc ctgtcgcctg gcaatctcct
     9481 tttgacgcac gggatcatac tgatcaggaa accgctccag gagctttttc cagtaaaggg
     9541 atgtggcctc aaagtggcgg cccacataaa tcagcgtccg cccgggtttg gccttgagcc
     9601 aatcctccaa ccaggtgacg acactttcgt gggggacatt cggatccgcg gcaaaccaga
     9661 ttaggcaatc ggcctgctct ttaagcatcg gacttaagtg tgtgcttcgg ctcaccgagt
     9721 gccccgaccg acgcaccatc tccgccaaca cccacgtgcc gttgatgctt ttccggccca
     9781 gccaccccat cgtgccgtat gtgacatcaa cctcgggcgc aggccaggga tagacggcca
     9841 acacgatggc caagaccgaa acgatgaccc cggcgagaat ccaaaatagg ggctttcgga
     9901 attccgcctg gggcgaatcg ttcatgccgc gatctccttc aaccactgtt gaaactgcgg
     9961 caattctcga cgacacgcct cgtattgctc ggcactcagg gaacgaccgc cgaaataggc
    10021 ctcttcaaag gtcacgatgg tccgttccag aagaggagcg accggcgact cgcgtcccaa
    10081 ctccctcagg tattgccggt tggtcttccc cttcatcagg cgaatcaggc gatgacgatc
    10141 caactcggct agttgatagg cataaagcca gataatggcc cgccgatagt ctcctgccgc
    10201 tgctgcccgc tcactggcct gcaaaagatc cgtctcttgc ggggagtctt ccggaacaag
    10261 gggcaactcg cgcaattggg cagccacccg cgctgcctct tccttgcctc cttggcaggg
    10321 ggcccatcgc cgccacgccc agtagcccaa aatgaccatg gcggcaatgg ccaaaacgat
    10381 taagatgatt cccactgccg accaagggac acgggagcga gaaaccttgg aaggctcagg
    10441 caactccctg ggcttccgca cgcgggtagg cttgatgtcg tccttctccg catcatac
//
LOCUS       DAOPTR010000010        10243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_5500_length_10243_cov_3.857381, whole genome shotgun sequence.
ACCESSION   DAOPTR010000010 DAOPTR010000000
VERSION     DAOPTR010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 10243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10243
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5500_length_10243_cov_3.857381"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PKI05_00425"
     CDS             <1..467
                     /locus_tag="PKI05_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HOA50687.1"
                     /translation="YVWVFLISGSAPPAHFIGGWAGASSERQPAMYFRLGGGTLKGIS
                     KPGEIVWSRVYVDNGRLCMDIGRAGVVELPPEETERRWKETTPQWPIMHAVTYGVTRD
                     QFMAKHKANHIQVAYAEDADAADAAALAKAAMADALGIRVNMCGTRKGGKHW"
     gene            529..2088
                     /locus_tag="PKI05_00430"
     CDS             529..2088
                     /locus_tag="PKI05_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOA50688.1"
                     /translation="MSEALQCVKKTAVITGWDFSTGGVKCLAFDIEGHVLAEVRLPTD
                     IWFGDSPATGIRELNVMQLEGQAYASTRAIAAELTRLGRIKDWVAGGISATHHTSARI
                     DRLGNQVRRAICWNDATLAEYHAKGLARLGGQERVRELIGGPWAIRYGLSHLVKDEVH
                     LSPADWRRTYRIAPHGVLAAGYLTGNFDVCSVSVGASSGIMDLRTKKWRWEMLEAIEN
                     EEYRRLVKKQLPKIIDQNTPIGPLSESVALSAGLPPRVRPLIFPTSDDQQAGLVGGGA
                     VDAGQVAVILGNSAVVNSSSADRPQRGQLDVMRLNWGPYLWMRCYNNGAQFLDQVVGP
                     QPNWGELEQAARNELPGCDGTLVLPFVNPEPSLGISQKDAQLRWIPQMPETMGRRFRA
                     SLEAIAYLIALAVREHEDAGQKITRITVSGGIARSNLMCEILATVLGRSLVRLESEEG
                     PALGAAVTALAALESHLRKAKGIRKKFTVADAVAAMVRFRGTVEPNPAWAEAYRMGFE
                     RFCNELARIKE"
     gene            complement(2236..2598)
                     /locus_tag="PKI05_00435"
     CDS             complement(2236..2598)
                     /locus_tag="PKI05_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOA50689.1"
                     /translation="MDVQNLAHVPAFITKDGSEIRELLSYRNSCIRNQSLAEARLPPG
                     RSTQGHHHVRSEEIYYILEGEAIMQVGEEKRQVGPGDAIAIPPGAYHQITNTGSTVLR
                     FLCCCAPCYEHDDTVLRD"
     gene            3029..3505
                     /locus_tag="PKI05_00440"
     CDS             3029..3505
                     /locus_tag="PKI05_00440"
                     /inference="COORDINATES: protein motif:HMM:NF021193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HOA50690.1"
                     /translation="MWTPLHAGLGGTDTPQINAAPENGAAAQLEELLASLTWFDRILL
                     RVGIRWLRRIAITIIGLTVVLVGIIMIVAPGPATLVIPIGLAILGLEYAWARRLLRRL
                     RGYVDAGITHGSALLGWKTPLVPSPAGTTPVQPPNESANTSQTPNTSTSPATTSSP"
     gene            3502..4002
                     /locus_tag="PKI05_00445"
     CDS             3502..4002
                     /locus_tag="PKI05_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOA50691.1"
                     /translation="MSRFIQQRHRQPITLERPRELIVACPPMKSNVNLSRIVRAAGCC
                     GIRRLICCGNARILPEIARDTGPDLQIEFHRSLLPVLKKLKAEGFPLIGLEQTTGSIW
                     IYEFAFPRRCVLVVGHERLGIEDDILAILDHVVEIPVYGLPFAHNVATATAIALYEYC
                     RQYPKG"
     gene            4346..>4752
                     /locus_tag="PKI05_00450"
     CDS             4346..>4752
                     /locus_tag="PKI05_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50692.1"
                     /translation="MSESREIVLINMPFAHLRWPNLGLGLLAGALKRRHLPCRTLYLN
                     FALAEEIGYDLYEWIADHYAFVLGGERLFSQHYFGQLPTDHQAYYEEVLHRADRDLTR
                     EEYAEYLAIFPKIERFIDAAFDAYPWQDAALVG"
     assembly_gap    4753..4762
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4763..6291
                     /locus_tag="PKI05_00455"
     CDS             <4763..6291
                     /locus_tag="PKI05_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HOA50693.1"
                     /translation="QTLASLALAQRSKRASPETVIVFGGAACEDEMGVELLRQFPEID
                     YVFLGEADETFPELAEAVLGGRRPPILPGVVARANMFGPSLQGTSESESVTGQPASPI
                     LPQLHILRGPRSVLVENLDALPYPDFDDYFATLQRSPLRHELTPMLFFEMSRGCWWGE
                     KHHCAFCGLNGQSLRFRSKSPQRVIDELRYLVTRYDVHQGCAADNILDFRYFASLLPE
                     LEKAQLAFSFACELKTNLKRSQIEQLLRAGLRAAQLGIETFDSEILKNLNKGVRGYQN
                     LQTLKWFAEARIAAEWNILYGFPWETADTYRQLASLVTKIPHFPSPLGVGRARMDRFS
                     PFFESPGRYGMKNPRASRAFAHVYPFPQDVLNRLAYYYEFDADWEPQLEFAEPFLEAI
                     RHWQNDSGSGDLTMHWKTPDELVIFDTRGGRMRQHRLRGLEAELYVTCDTAQTLQTLT
                     QVVATRSITPENLKALLSQWLEDDLMIYWDGRFLSLALWVPEQSPLYQAAQANLEPIP
                     "
     gene            complement(6436..7755)
                     /locus_tag="PKI05_00460"
     CDS             complement(6436..7755)
                     /locus_tag="PKI05_00460"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOA50694.1"
                     /translation="MNTPGSLRWHFLYSTMAAVIGLAVALSWSTSSWAMHREQHIFSI
                     VDFGAVPNDEQLDTESVQKAIDKAAEVQGTVRIPAGRFILGNVQLRSGIRLVFEEGAV
                     IQGSSDWRHYGQGRWTDSLFLGEDITNLRIEGPGTIDGADCERPGGEEGFRGPHGIRL
                     VRSSGVVINDVTIKRAGNYAIICWECRDVQVKGVTFRGGHDGVHAQACQNFVIEACDF
                     RTGDDCIAGCDNEHFVIRNCQINSSCNGFRLGCVHLLVENCRFWGPGEYPHRITVRQG
                     KPRTNMLSAFVHFAPTDRNPNLPSDDWLVRNCTMTNVDRVYSYDFEKGVWQTGQPAKR
                     LVFENIQAEGIAFPLNILGDKDRQFDLTLRNVAISLRPASAQQPVIQARQFGKITLER
                     VVLKNDGSQPVLRAREGNELILQNVAYPPECSQPFLLEDIVRIDRRR"
     gene            7942..9429
                     /gene="hemG"
                     /locus_tag="PKI05_00465"
     CDS             7942..9429
                     /gene="hemG"
                     /locus_tag="PKI05_00465"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HOA50695.1"
                     /translation="MENTLPSPDAPARPFRVAVIGAGISGLAAAWQIRRLRPEAELAV
                     FEQQHSVGGVLGTVRENGFQVERSADNFITTLPWGVQLCRELALEDQLVTTHPDHRRA
                     FVVWRGRLHPLPDGFLMMAPTRLWPLATTPLLSPLGKVRAAMEFFIPPRRDDNDESLA
                     HFARRRLGREVYERLVEPLVSAVYGADMERLSVLATLSRFREMERNYGSLIRAMRVQM
                     AQRRRAGTSRDGHSRESGARYSMFVTLQQGLQELVDRLAQHLPPGALRLGVGVTTIRR
                     ENDHWVLTLTDGHQFVGDAVVVATPAYAAAPLLCELDGRLAEELAAITYTGSVILAVA
                     YRREAVGHPLHGSGCVIPRIEGSPLLAVSFSSQKYPHLAPKGYVLLRVFAGGARHPEL
                     VSLEDTQLVPLLRKEVERLLRIEGQPVQHWLFRWSQAMPQYEVNHGDRLRRIAERLAA
                     WPGLALAGNAYRGVGIPHCIQSGRQAAVRALGLPVEDQQTSSSAF"
     gene            9460..9672
                     /locus_tag="PKI05_00470"
     CDS             9460..9672
                     /locus_tag="PKI05_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50696.1"
                     /translation="MRKNDGQENDSPRFSWLHNVDVPFDWLLGTVTGEVGLADSVDML
                     QFDQLFPRLLAHSLSVTGTGVIAFGI"
     gene            complement(9878..>10243)
                     /locus_tag="PKI05_00475"
     CDS             complement(9878..>10243)
                     /locus_tag="PKI05_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50697.1"
                     /translation="NNKPAGQLASAQVARVQRLTPAELDPVTRMIHWPAALQGDRYAK
                     QREALEELFTNRQPNEHGAASPFFAKVRENVDVMKNLLREEVTKLSPAEYIAASKFLD
                     GLRNEALYPAQINGVAMNQ"
BASE COUNT         2090 a         2881 c         2985 g         2277 t
ORIGIN      
        1 attatgtatg ggtcttcctt atcagtggct cggcaccacc ggctcacttc attgggggct
       61 gggcgggagc atccagcgaa cgtcaaccgg cgatgtattt tcgcctgggt ggcgggaccc
      121 tcaaaggaat ttccaagccg ggggaaatcg tctggtctcg ggtgtacgtg gacaacggcc
      181 ggctgtgtat ggacatcggt cgggcaggcg tggtggagct tcctccggaa gagaccgaac
      241 gccgctggaa agagacgaca ccccagtggc cgatcatgca tgccgtcacc tatggcgtca
      301 cgcgggatca attcatggcc aagcataagg ctaatcacat ccaggtagcg tatgccgagg
      361 atgccgatgc cgcggacgca gcggctctgg ccaaggcggc gatggccgat gccttgggaa
      421 tccgcgtcaa tatgtgtgga acacgcaaag gtggaaaaca ttggtgactt gccgtcatct
      481 ttgctggtga gattccgggc cgagatcgcc gtataggagg ccaatgtgat gtccgaagcg
      541 ctacagtgtg tcaaaaagac ggccgttatt acaggctggg attttagcac cggtggtgtg
      601 aagtgtttgg ccttcgacat tgaaggccac gtcttggcag aggtgcggtt gccgacggat
      661 atttggttcg gggatagtcc ggccacgggc atccgcgagt taaacgtgat gcaattggaa
      721 ggtcaggcgt atgcatccac gcgggccatc gcagcggagt tgacgcgact gggacggatc
      781 aaggattggg tcgccggggg gatttcggct acccaccata cgtcagcccg catcgaccgc
      841 ttgggcaatc aagttcgccg ggcaatctgc tggaacgacg ccacccttgc agaatatcac
      901 gcgaaagggc tggctcgact cggtggccag gagcgcgtcc gcgagttgat tggcgggcct
      961 tgggctatcc gttacgggtt aagtcacttg gtcaaggatg aagttcacct tagcccggcc
     1021 gattggcgcc gaacttaccg catcgccccg cacggggtgc ttgcggcggg gtatcttacg
     1081 ggcaattttg atgtgtgcag cgtctccgtc ggggcctcct ccgggatcat ggacttgcgg
     1141 acgaaaaagt ggcgctggga aatgctggag gctatcgaaa acgaagagta tcggcgtctg
     1201 gtcaagaaac aattgcccaa gatcatcgat cagaacacgc cgatcgggcc gctctcggag
     1261 agcgtggcgc tttcggccgg cttgccgccg cgcgttcgac ccttgatttt ccctacctca
     1321 gatgatcaac aggcagggct tgttggcggc ggtgctgtcg atgcgggaca ggtggccgtc
     1381 atcttgggaa actccgccgt tgtcaacagt tcatcggccg atcggcccca gcgtgggcag
     1441 ctcgatgtca tgcggctcaa ttgggggcct tatctgtgga tgcggtgcta caacaatggc
     1501 gcgcaatttt tggaccaggt ggttggtccc cagccgaatt ggggtgaact ggaacaagcg
     1561 gcccgcaatg aactgcctgg ttgcgatggc accctcgttc tgccttttgt caatccggag
     1621 ccgtcgcttg gcatctcgca gaaggatgcc caactccgtt ggattccaca aatgcccgaa
     1681 acaatgggac ggcgttttcg ggcatcgctg gaggccattg cctatctaat cgccctcgcc
     1741 gttcgggaac acgaagacgc tgggcaaaag attacccgca ttacggtctc cggtgggatt
     1801 gcccgaagca acctcatgtg tgagatcctc gccacggttc tcggtcggtc gctagtgcga
     1861 ctggagtcgg aagagggacc cgcgttgggg gccgctgtga cggccttggc ggccctggaa
     1921 tcccatctcc gcaaggcaaa gggaatccgg aagaaattca ccgtcgccga cgcggtggcc
     1981 gctatggtcc gcttccgcgg gacggtcgaa cccaatccag cgtgggcgga ggcctatcgg
     2041 atgggcttcg aacgcttttg taacgaactt gcgcggatca aggagtaagc gtccgccccg
     2101 acgtgcctga cgtttgccaa gcgatgcacc gggctaatcc tggaggaaat ggagcaacgt
     2161 tggcgatcgg tctctgggac tcgatggtat tttgccacga caagcccgcg ggttcccccg
     2221 ggcacagttt ggggatcagt cccgcagcac ggtgtcgtcg tgttcgtagc acggggcgca
     2281 gcaacagaga aagcgcagga cggtgctccc ggtattggtg atctggtggt acgcccctgg
     2341 gggaatggct atggcgtcgc ccgggccaac ctgccgcttt tcctcgccca cctgcatgat
     2401 ggcctcgcct tcgaggatgt agtagatttc ttccgatcgc acatggtggt gtccctgcgt
     2461 acttcgcccc gggggcagac gggcctcggc caaagattgg ttgcgaatgc acgaattcct
     2521 atatgagagg agttcgcgga tttccgagcc atctttggtg ataaacgcgg gaacatgagc
     2581 aagattttga acatccatca gggttgctcc tcgaccgaac tctctagccc atccggtatt
     2641 cggcgtaccg gtaacagcca actcaaggat attatgataa ataaagggca tgtccagggg
     2701 gtaagcggga taatcgccca acagccagtg aggcttacgg gcgcactcgc aaggatgatt
     2761 gacttagagc gtatcttaga attccctctc cctctgggag agggttaggg tgagggcaag
     2821 ccatcgtcgg agccgatcgg aacaccagca gcgattcgag ccaccctcac ccccagcccc
     2881 tctcccgccg aacgggcgag gggaggttgt gggataggct cttaacagga ctgtgaccga
     2941 gccatcggga tgttttcagt tcaacgttcg cctaaggact cgcgccggta gccctggata
     3001 aaaaggcaag ataaaaggat aaatcaccat gtggacgcca cttcatgccg gattgggtgg
     3061 aacagacacg ccacaaatca atgcggcacc tgagaatgga gcagcggcgc aattggaaga
     3121 acttttggcc agtttgacat ggtttgaccg gatccttttg cgggtgggca ttcgctggct
     3181 tcggcggatc gccatcacca tcatcggcct gacggtagtc ttggtgggca tcattatgat
     3241 tgtggcccca gggccggcaa cgctcgtcat ccccattggc ctggccatct tgggtttgga
     3301 atacgcttgg gcacgacggc ttcttaggcg actgaggggc tatgtggatg ccgggatcac
     3361 ccacggctcg gccttattgg gatggaaaac cccgctcgtc ccatctccgg ccgggacaac
     3421 ccctgtgcag cctccgaacg agtcagccaa cacctctcaa acgcccaaca cctcgacatc
     3481 acccgcgaca acgtcctctc catgagccga ttcatacagc aacgccatcg acaacccatc
     3541 acactggaac gcccgcggga actcatcgtg gcctgcccgc cgatgaagag taacgttaat
     3601 ctttcccgca ttgttcgggc ggcgggatgc tgtgggattc gtcgtctcat ctgttgtggt
     3661 aatgcccgca tcttgccgga gatcgcgcgg gacaccgggc cagacctcca gatcgaattt
     3721 catcgctctc tccttccggt cctgaaaaag ctaaaggccg agggtttccc gctgatcggc
     3781 ctggaacaga ccaccggctc gatatggatt tatgagtttg catttccccg ccgctgtgta
     3841 ctcgtcgtcg gccatgaacg cctcggtatc gaggacgaca tcctggccat cctggaccac
     3901 gtggtggaaa tccccgttta cggactgccg ttcgcccata atgtagccac agcgacggca
     3961 attgcgctgt atgagtattg tcgtcagtac cctaaaggct aaaatcagcg aggaagtttg
     4021 taaccgcccg gggaattgag tcactgtcct gtattaagag cccatcccaa aattccctct
     4081 ccctctggga gagggttggg gtgagggcaa gccatcgtcg gagtcgatcg agaaaaccag
     4141 cagcgatccg agccaccctc acccccggcc cctctcccgc cgaacgggag aggggcggtt
     4201 gtgcgatagg ctctaacacc gtcaccgctg cgcgagatag gtttcatgct gtgggaacgg
     4261 catttcgtgc caggttcccg gataaacaga taaaagacat cggtctctga taacgagaac
     4321 ccatccttta ttcgtatagt ttggtatgtc ggaaagccgc gagattgtcc ttatcaatat
     4381 gccgtttgcc caccttcgct ggccgaattt ggggctgggc ttgcttgcgg gggctttgaa
     4441 gaggcggcat ttgccgtgtc gtacgcttta tctcaatttt gctttggcgg aagaaatcgg
     4501 ttatgacctg tacgagtgga ttgccgacca ttatgccttt gtcctcggcg gcgaacgcct
     4561 cttctcccaa cactatttcg gacagttgcc cacagatcac caggcctatt acgaggaggt
     4621 gcttcaccgg gcggaccgcg atctcacacg tgaggaatac gccgagtatc ttgccatctt
     4681 ccccaagatt gagcggttta tcgacgcagc cttcgatgcc tatccttggc aggacgcggc
     4741 acttgtagga ttnnnnnnnn nnagcaaact ttggcctcgt tggccctggc gcagcggagc
     4801 aagagggcgt ctcccgagac ggtcatcgtc tttggcgggg ccgcttgcga ggacgagatg
     4861 ggcgttgagc tccttcgcca atttccggaa atcgactacg tcttcctcgg tgaggccgac
     4921 gagacatttc ccgaactggc cgaggccgtg ctaggtggcc gaagaccacc aattcttccc
     4981 ggcgtcgtgg cccgagcgaa tatgttcggc ccttcactcc agggcacatc cgaaagcgaa
     5041 tccgtcacag gtcaaccggc atctccgata ctgccacaat tgcacatttt gcgaggaccg
     5101 cgatccgtct tggtcgaaaa ccttgacgcc ctaccgtacc cggattttga tgactacttc
     5161 gcgacgctcc agcggagtcc attgcggcac gaattgaccc cgatgctttt ctttgagatg
     5221 tcccgcggat gctggtgggg tgaaaaacac cactgcgcgt tttgtgggct aaatggccag
     5281 tcgttacgat ttcgcagtaa atccccccag cgcgtgatcg atgaattacg ctatctcgtc
     5341 acccgctacg atgtccatca gggttgtgcc gcggacaaca tcctggactt ccgctacttt
     5401 gcctcgctat tgcccgagtt ggaaaaggct cagctagcgt tttctttcgc ttgtgaactg
     5461 aaaaccaatc ttaagcgttc tcagatcgag caacttctac gcgcaggact tcgggcggct
     5521 caattgggta ttgagacgtt cgattcggag attctcaaaa acctcaacaa aggcgtccgt
     5581 ggatatcaaa atcttcagac gctcaagtgg tttgccgagg cccgcatcgc cgcagaatgg
     5641 aacatcctct atggctttcc ctgggaaaca gccgacacgt atcgccagct cgcgtctttg
     5701 gtcacaaaga ttccgcattt tccgtcacca ttaggcgttg gtcgggcacg catggatcgc
     5761 ttttcgcctt tcttcgaaag tcctgggcgc tatgggatga aaaaccctcg tgcttctcgc
     5821 gcgtttgccc atgtctatcc ctttccccaa gatgtcctca accgcttggc ttactactac
     5881 gaattcgacg ccgattggga accgcagctt gagttcgccg agccatttct agaggccatc
     5941 cgtcactggc agaacgactc cggcagtggc gacctgacaa tgcactggaa aacccctgac
     6001 gagttggtca tatttgacac gcgcggtggc cggatgcggc aacaccgcct gcgggggctg
     6061 gaggccgagc tgtacgtcac atgcgatacg gcccagacgc tgcaaacgct tactcaagtt
     6121 gtggccaccc ggtcgataac accagagaac ctgaaggccc ttctcagcca gtggctggaa
     6181 gacgatctga tgatctactg ggatggtcgt ttcttgagtc tcgccctctg ggtacccgaa
     6241 caatcgcctt tatatcaagc ggcccaggcc aacttagagc ctatcccata atcccccctc
     6301 gcccgttcgg cgggagaggg ggcaggggtg agggtcgctc gtatcgctct cggttttctc
     6361 aatcgattcc gacgatcgct tgccctcacc ccagccctct cccggaggga gagggggact
     6421 ttttgggatg gactcttagc ggcggcggtc gatgcgaaca atatcctcca ataaaaaggg
     6481 ttgggaacac tccgggggat aggccacgtt ttggaggatc aattcatttc cttctcgggc
     6541 acgaagcacc ggctggctcc catcgttttt taagacaacg cgttccaagg tgattttccc
     6601 aaattgtctg gcctgaatga cgggttgctg tgccgaagcc ggacgaaggc tgatcgccac
     6661 attccgcagg gtcagatcaa actggcggtc cttatccccg agaatattca atggaaaggc
     6721 gattccctcg gcctggatat tctcgaatac tagccgtttg gccggttgac ctgtctgcca
     6781 aacacccttt tcaaagtcat aagagtaaac gcggtcaaca tttgtcatcg tacaattgcg
     6841 gacaagccag tcatcgctgg gcagatttgg attacgatcc gtcggtgcaa aatgcacaaa
     6901 ggccgacagc atgtttgtcc gcggcttgcc ctgccgaacc gtaatgcggt gaggatattc
     6961 ccccggcccc cagaatcggc aattctccac taaaagatgg acacatccca accgaaaacc
     7021 attacacgaa gaattgattt gacaatttcg gataacaaaa tgctcattgt cacatccggc
     7081 aatacaatca tcgccagtac ggaaatcgca ggcttcgatc acaaagttct gacaggcctg
     7141 ggcatggacg ccgtcgtggc ctccgcgaaa tgtcacacct ttgacctgca catcgcggca
     7201 ttcccagcaa atgatcgcgt aatttcctgc ccgtttgatc gtcacatcgt tgataacaac
     7261 cccactcgag cggacaaggc ggattccgtg tggcccacga aagccttcct cgcctcctgg
     7321 tcgctcacaa tcggcaccgt caatcgtgcc tgggccttcg atccgaaggt tcgtgatatc
     7381 ctctcccaga aagagcgaat cggtccagcg tccttggccg tagtgtcgcc agtcgctact
     7441 gccctggatg actgccccct cctcaaacac caaccggata ccgctccgca actgaacgtt
     7501 gcccagaatg aaccgtcctg ccgggatgcg aaccgttccc tggacctcgg cggccttgtc
     7561 gatggccttc tgaacagatt cggtatcaag ctgttcgtcg ttgggaacgg ccccaaaatc
     7621 gacaatggag aaaatgtgtt gttctcggtg catggcccaa ctcgacgtgc tccaagagag
     7681 ggcgaccgcc aacccaatga cggcggccat cgtagaatat aaaaagtgcc agcgcagact
     7741 accgggagtg ttcataatga ttttccccac aatcttttgt ttgcggtgtt ctcccgagat
     7801 gaccatgcct ctcgtggcat gggaccgcca tcggttataa gccaagtttt ccatcgatcg
     7861 atagtgggag agacaattgt cacaacacca tgtccccggt tattgtctgc caagagtaac
     7921 agattgagaa gatcacccga tatggaaaat acgctacctt ccccggatgc ccccgccagg
     7981 ccgtttcgtg tcgccgtgat tggtgctgga atcagcggcc tggcggcggc atggcagata
     8041 cgccgccttc gtcccgaggc tgagctcgcg gtttttgaac agcagcattc ggtgggaggt
     8101 gtgctgggaa cggttcgcga aaacgggttt caggtcgagc gcagcgccga taatttcatc
     8161 accacgttgc cctggggtgt ccaattatgc cgtgaattgg ctctcgaaga ccaactggtg
     8221 acgactcacc ccgatcatcg ccgggcattc gtagtgtggc ggggacgcct ccacccgctg
     8281 cctgatggtt ttctcatgat ggcgccgacc cgcctttggc cgctggcgac gacgcctctt
     8341 ttgtcgccct tgggcaaagt gcgtgcagcg atggagttct tcattccccc tcgccgggac
     8401 gataacgacg aaagcctagc ccattttgcc cgtcgccgtt tgggacgcga ggtttacgag
     8461 cggctggtag aaccgctagt ctcggcggtt tacggggcgg acatggaacg gcttagtgtg
     8521 cttgccacgt tgtcgcgttt tcgtgaaatg gaacgtaact atggaagcct gatccgggcc
     8581 atgcgcgtgc aaatggctca gcgacgccgg gccggtactt cacgagatgg tcactctcgg
     8641 gaaagcggtg cccgatacag catgttcgtc acactgcaac aagggttaca agagcttgtc
     8701 gatcgacttg cccagcattt gcccccaggt gcccttcggc ttggtgtggg tgtcacaacg
     8761 attcgccgcg agaacgatca ttgggtcctg acgctgaccg acggtcatca attcgtcggc
     8821 gacgcggtgg tggtggccac cccagcctac gccgctgctc cccttttgtg cgaactggat
     8881 ggccgtctcg ccgaggagtt ggccgccatc acctacaccg gcagcgtgat cctcgctgtc
     8941 gcgtatcgcc gcgaggcggt agggcatccc ttgcatggct caggatgtgt catcccgcgg
     9001 attgaaggca gcccccttct ggccgtcagc ttcagcagtc agaagtaccc ccacctcgcg
     9061 cccaagggtt acgtcctact tagggtgttc gctggaggtg cccgacatcc agaactcgtg
     9121 tctctggaag acacccaact tgttccgctc cttagaaagg aggtcgaacg cctgctgaga
     9181 attgaggggc agcccgtgca acattggctc ttccgctggt cgcaggccat gccgcagtac
     9241 gaggttaatc acggggatcg tttgcgtcgg attgccgagc gactggctgc ttggcccggc
     9301 ctggccttgg cgggaaatgc ctatcgcggc gtggggattc ctcattgcat ccaaagcggt
     9361 cggcaagcgg cagtgagggc cttgggactt cctgtcgagg accagcaaac aagttcttcc
     9421 gcattttgat ctgtcctgcc cagcggctgt ccaggtggaa tgcgaaaaaa cgacggccaa
     9481 gaaaacgact caccccggtt ttcctggctt cacaacgttg acgtgccttt cgattggttg
     9541 ttgggaacag ttacaggcga ggttggtttg gccgatagcg tggatatgct tcagttcgac
     9601 cagttgttcc cacgattgtt ggcacactcg ttgtcagtta cagggaccgg ggtgatcgcg
     9661 tttgggattt gattttcggc aggactgacg aaccacactt aggaaggatg ctgcactttg
     9721 ctaggactct tggctcgtca cacgttcagt tactttcagg taggacactc aaggttggtt
     9781 aactcagagg tgggactttt caggtaggat gttgaggaag gtttgttggg gtttaggttt
     9841 gctgtagggt aggctgactg tcacacattg gtgagcctta ctgattcatg gccacgccat
     9901 tgatttgtgc ggggtagagg gcttcgtttc ggagaccatc gaggaacttc gaggccgcga
     9961 tgtattcggc gggggagagt ttggtaactt cctcgcggag caggttcttc atgacatcca
    10021 cgttctcccg aaccttggcg aagaaggggc tggcagcacc atgctcgttc ggctgacgat
    10081 tggtgaatag ctcctcgaga gcctcgcgct gcttggcata gcgatcgcct tggagggctg
    10141 ccggccagtg aatcatccgg gtgacaggat ccaactccgc aggcgtgagc ctttgaactc
    10201 gggcaacctg ggcggaggcc agttgaccag ccggcttgtt gtt
//
LOCUS       DAOPTR010000011        10222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_5514_length_10222_cov_3.957706, whole genome shotgun sequence.
ACCESSION   DAOPTR010000011 DAOPTR010000000
VERSION     DAOPTR010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 10222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10222
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5514_length_10222_cov_3.957706"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1092)
                     /locus_tag="PKI05_00480"
     CDS             complement(145..1092)
                     /locus_tag="PKI05_00480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOA50698.1"
                     /translation="MTSYRPFRNYDPPKLLRIWSECAGKPGLIQPLTLPYLERSVLNK
                     PYFDPNGLFLAFEDDQPVGFAHAGFLPQATQGELAREQGITCMLMVSKACDQQVVANE
                     LLDRCEDYLARGGARVLYGGSPNPCHPFYLGLYGGFRLPGIMQNDTLLTTLFGARGYQ
                     VVGGYLRFQVSRDQCRHLLDSDIIGRFSGFPIQRTTDPVPASWCEALCFCESEVIQYT
                     LINPANQQRIARVRFCRLDPNPHPRPAVGLAEVRTAAGWANTGADSFLMEESFSSLWA
                     EGIGVVEAQCCQSDVTMVQMLREFGFQQRTSGVVYRKSI"
     gene            1849..3648
                     /locus_tag="PKI05_00485"
     CDS             1849..3648
                     /locus_tag="PKI05_00485"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOA50699.1"
                     /translation="MLKVNLVQLPIPPLGPEIVRGNVPLASAYLKRYAQRVAGVDVEI
                     EILTADLTDRAGDAALVRELSQRGPNLVGFSCYLWNVERSLWLAERLKALHPELRVVI
                     GGPEVTLDNTWVLEHPAIDWAVVGEGEATFADLLKALTAEPRWLQHPTELPPIPGLWN
                     RATHSFGGARSQLPTLNEVGRVYLDGLLDPRWHDTLFLETIRGCIFQCAFCYYPKSFE
                     GLRFMDEDLVLANLHLAHQAGISEVFLLDPTLNQRRDFLSFLKLLARGNPERRFSYSA
                     ELRAEGIRAEHAGIMKAANFAEVEIGLQSVSREAQGLMHRRVNYQAFEEGVRALLEAG
                     IRVRADLIIGLPGDTAETIRQGFRYLLSLGLSNEPQIFRLSVLPGTELRRRSKELGLD
                     YQPHPPYHVLGTSKLSIVEIAQLMREAEFSFGRRFDPWPVSDEEFSPTILKERGSFWE
                     INLDRPEPTELPDIQIAHTLWIKARHFAGRAKDVRYWADRVLNENPHVTLWIVLDISD
                     AQTLPEEDDIQSILRACYDREHSYWDWYFAPELPPGPSGAKRLVVYSGRSLKGRRRWL
                     RQRFSPSADLVEAPECQAKGRGRRRRTCDSGNE"
     gene            3662..4072
                     /locus_tag="PKI05_00490"
     CDS             3662..4072
                     /locus_tag="PKI05_00490"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOA50700.1"
                     /translation="MESEPKTQIVEQVAIVVVCRDDHVLVGIRPDGVPFAGLAEFPGG
                     RLQPGESWEEAARRETLEETGLSVRVEALLGTVSTRYEDRILELRFFAASPVSPAASP
                     REPFRWIRRDALRADCFPPANRTIIEQIQKGHGQ"
     gene            5178..6260
                     /locus_tag="PKI05_00495"
     CDS             5178..6260
                     /locus_tag="PKI05_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOA50701.1"
                     /translation="MSEIKTHPASGVKKCICLGLSWAMVIFSAAIWDGIALGQTPAVL
                     SSFHVVDVTKRGDLRPPVITGLYYDAARDILITAGDDHLIRIFKQPGDQQLAVLNGQG
                     WIYGLVLDPTGKYLAAVDDRGLLTVWQWETARQLYRLPLSTAALRTVAFHPGGNWLAV
                     GGFEPRVFIVETLTGRRVADLNADRDVRQVVFSSDGASVVAAGGSGKLRLWDLPSLRQ
                     RMEITVSSRRLHAVAFSPDGRILAAGGEEPVVSLWQVDCDPPSLIGRLPLSDGIVTSL
                     TFCENGQQLAVGTTRNVIHVFDLVTSKELAQLEGHTGTISCLTWTPERSLLSSGSFDT
                     TVRFWRLLPGTTVSERLPTAQDILRQ"
     gene            6330..10061
                     /locus_tag="PKI05_00500"
     CDS             6330..10061
                     /locus_tag="PKI05_00500"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HOA50702.1"
                     /translation="MKGWKGRPQQAPSQKRRDRHPSRYRKCRLEQMEPRMLLSVSPIQ
                     LGMVYYEDATGTDESGDTFELTFQGGAPGTQLTRLVINTDKLGDGLSIGDCFFDTEPG
                     GLGAFGYGPFAVISASGIDNFRVSVSDGGTELVLEFTGFEAGDRFIFTIDVDEMGFLG
                     PNAVAEGNEFEGSRLTAYFVAPHYYAAQGEDIFIDAYDHKLSASGLVLPHDSYSPPLP
                     EPEPVRTAGAFLEVTQTPLPVAISGRVFEDFNLDLTLNGEDRPLAGVTLELWQWQGET
                     YIFTGRTTVTDADGSYRFDDLLPGTYRIVERQPEGFLSVGAKAGSVGSEVRGSVLTSD
                     IIVGISLLGGEESIENNFAEVRPAELSGHVYHDVNNNGLLESGETGIGGVTVIVIREG
                     DHFLTEQEFTVITQTDGSWSVVDLYPGKYRVQEIQPAGWLDGLDRAGSLGGSAVNPGD
                     QIVNIKVPAGSQGVNYDFGELLPASLAGLVYLDLNTNCQYDPSEPVLPGVTIKLLSPD
                     GTVLRTTVTDASGRYVFENLTPGTYALHEIQPEGYFDGCDRPGTAGGVKSPPDLITDI
                     HLASGTAATDYLFGEIPPASVAGLVFSDLNANDQLDAEDAPIAGVTIQLFAANGELVA
                     TTVTDAEGRYRFENLRPGYYTIVEVQPNGYLDGADFVGSAGGQRDGSDRITDIKLPPG
                     VHATDYNFAEILPATISGYVFQDGPTITYVWGQERPDPYLVRDGQFTPDDIPISGVEI
                     QLRDAVGQPIVGEDGRPLVTWTDERGYYEFRGLRPGVYTLLEIHPAGYEDGLDTPGTL
                     GGIAVNPNASLDPAILAALTIDPRNDAILLVTVTPGAVGTNYNFAEVVVTSVPPYVPP
                     TPPPPWSPPERPASPGVPPGTPPIHLQISPVMPESVALPRFGGSAGTVDYTWHLSVIN
                     GGVPRHNLGGYQRVLIREVSLFNPARWEGIPLDRGQWTIVDIITGETTALGNLGTEAA
                     LPVVGDFSGIGRVQLGIFYGGMFFIDLNGNGIWDDQDLWIQLGNDRDQPVAGDWDGDG
                     KWDVGIFGPAWPGDDRALRAEPGLPDAQNRLTAFRPRNLPPTPDQAPDDQRLLQQGRN
                     GPTRADLIDHVFMYGQPGDHAVVGDWNGDGIATVGVFRGGTWYLDEDGDGRWGSRDRV
                     AFFGQAGDLPVVGDWNGDGITDLGVYRDGRFYLDIDGNGQLTAADKVITLGQPGDRPF
                     AGDFNGDGRAEVGVYRTQSPSTGGGQTLREAAAPRGVAK"
BASE COUNT         2116 a         2995 c         2984 g         2127 t
ORIGIN      
        1 cttgcccatg gtgttggaag cctgacatgc gcaaaaacgc acgcgccccg aacccaccga
       61 ttatgcgatt gttcatccta ccagcgtcaa gaagaccaag ccagcggtgt aacaagccga
      121 atgcgcagca aagcgaagaa tcagtcaaat agacttccgg taaacaactc cgctggtgcg
      181 ctgctgaaaa ccaaactctc gaagcatctg caccattgtc acgtcgcttt ggcaacactg
      241 ggcctccaca acgccaatgc cctcggccca aagcgaggaa aaagactcct ccatgaggaa
      301 cgaatcggcc ccagtgttag cccaaccggc ggcggtgcgc acttctgcca ggcccacggc
      361 cggtctgggg tgaggattag gatccaggcg gcaaaaccga acgcgagcaa ttcgttgctg
      421 attggccgga ttgatcaatg tgtactgaat gacttccgat tcacaaaagc aaagggcctc
      481 gcaccaagag gcgggcacag gatcggtcgt tcgctgaatg gggaagcctg agaaccggcc
      541 aatgatatcg ctatcgagga gatggcgaca ttgatcccga ctcacttgaa aacggaggta
      601 tccacccacg acctgatacc cccgagctcc gaaaagcgtc gtaaggaggg tatcattctg
      661 catgatcccc ggcaatcgaa aaccgccgta aagaccgaga taaaagggat ggcagggatt
      721 tggcgacccc ccgtaaagga cccgcgcacc accgcgtgcc aaataatctt cgcagcggtc
      781 gagcaattcg ttggcaacca cctgctgatc acacgccttg gagaccatca acatgcaggt
      841 aatgccttgc tcgcgggcaa gttctccctg ggtggcctgg ggaagaaacc cggcgtgggc
      901 aaaccccaca ggctgatcgt cctcgaaagc caaaaacaaa ccgttcgggt cgaagtacgg
      961 cttgttgagg acactccgtt caaggtaggg gagggtcaat ggctgaatca gccctggctt
     1021 gccggcgcac tccgaccaga tgcgcaacag ttttggcggg tcatagttcc gaaaaggtct
     1081 gtagctagtc attcgcccaa tgctccgaaa ggcgcttccc gacgttgtgg caacatcgca
     1141 tggaatgggt gagggaccac ccgaaatttg gctggattgt gtggatgaac ttgccttccc
     1201 ttttcaatcg tccactgact cctctcagtc caattttaac aggtggactc gtggagagac
     1261 cataccaagc cgaatgggcc tcgacacagt tttaccgcag tcgagcaaat cgtggcggtt
     1321 gttcgccggg accatcactg gggaaccccc agggttaccc ctcaccagtt tgcagggcag
     1381 ctcaggacac agtggacgcg gagccacgcg gctttccaga tttcaaggaa cacaacgggg
     1441 aaggaaacgg ggcaagccca agcgtcatct ttctgtgggg aaccgcacaa accctatgac
     1501 agaaacccct gccgccggcc ccctctgcga gtcatctcct cacaccttct cgcggccgtc
     1561 tatacttaaa agaacgctgg tggatcccca tcgcaaaagt ctgccattgg caaggtgcta
     1621 aggcctatcc caaaaaagtc cccctctccc tctgggaaag ggttggggtg agggcaagcg
     1681 attgtcggaa tcgatcgaga aaacccgcag cgattcgagc caccctaacc cccaacccct
     1741 ctgccgccga acgggcgaga ggagattatg ggataggctc taactaggag catgaactgg
     1801 tccgttctag catctgggct ttcactacaa tcaaggcagg tgtcccacgt gctcaaagtc
     1861 aatctggttc aacttcctat cccacccctt ggccctgaaa tcgttcgcgg caatgtcccg
     1921 ctcgcctccg cttatctcaa gcgatatgcc cagcgcgtgg caggggtcga tgtcgagatc
     1981 gaaattctca ccgccgatct caccgatcgg gcaggggatg ccgccctcgt gcgggagcta
     2041 tcccaacggg ggcccaacct cgtcggcttc tcttgttatc tgtggaacgt tgagcggtcg
     2101 ttatggctgg ccgagaggct aaaggcactg catcccgagc ttcgcgtggt tattggtggt
     2161 cccgaggtca ccctggataa cacctgggta cttgagcatc cggcgattga ctgggccgtg
     2221 gtcggcgaag gtgaagcaac ttttgccgac ctcctcaaag ccctgacagc cgagccgagg
     2281 tggcttcaac atccaacaga gctcccaccc atcccgggac tttggaaccg ggcaactcat
     2341 tcgttcgggg gagcacgttc ccaactgccg actttgaacg aagttgggcg ggtctatttg
     2401 gatggcctcc ttgacccgcg atggcacgac accctgttct tggaaaccat ccgcggctgc
     2461 atctttcagt gtgctttttg ctactatcct aagagttttg aaggccttcg gttcatggac
     2521 gaggatttgg tcctagccaa cctccacctt gcccaccaag cggggatcag cgaggttttc
     2581 ttgctggacc cgacgctcaa ccaacggcga gattttcttt catttcttaa gctcctggct
     2641 cgcgggaatc ccgaaagacg gttctcttac tccgcggaac tgcgtgccga agggattcgg
     2701 gccgagcacg ccgggatcat gaaggccgcc aatttcgccg aggtggagat cggtctccag
     2761 tccgtaagcc gcgaggccca ggggctaatg catcgtcggg tcaactatca ggcctttgaa
     2821 gagggtgttc gcgccctttt ggaggccggg attcgtgtcc gggcagacct gattataggc
     2881 ctgccgggag atacggcgga gacgattcgg cagggattcc gctatctcct ttcgttgggc
     2941 ctttctaatg aaccccaaat ctttcgtctt tcagtgcttc cagggaccga gcttcgtcgc
     3001 cggagcaaag aactagggct ggattaccag ccgcatccgc cgtaccacgt cctggggaca
     3061 tcgaaacttt ccatcgtgga aatcgcccag ttaatgcgag aggcggaatt cagttttggt
     3121 aggcgcttcg acccctggcc cgtttccgac gaagagttca gtcccacgat ccttaaagag
     3181 cgcggcagct tttgggaaat caatctcgat cggcccgagc caaccgagct tcccgacatt
     3241 cagatcgccc acacgctgtg gataaaagcc cggcattttg caggccgagc caaggacgtt
     3301 cgctactggg ctgaccgagt gctcaacgag aacccccatg tcacgctttg gattgtgctt
     3361 gatatcagcg acgcacaaac cttacccgag gaggacgata tccagagcat ccttcgggcc
     3421 tgctatgacc gggaacacag ttactgggat tggtatttcg cgccggaatt gcctcctgga
     3481 ccatcgggag caaaacggtt ggtggtttac agtggccggt cactcaaagg ccgacgccga
     3541 tggctcaggc agaggttttc tccctcggcg gatttggtcg aagccccgga atgtcaagcc
     3601 aaaggccgcg gcaggcgaag aaggacttgc gattcaggaa atgaataggg tgggcatttc
     3661 gatggaatcg gagccaaaaa ctcaaatagt cgagcaggtt gccattgtag tggtgtgccg
     3721 ggatgaccac gtcttggtgg gaattcggcc tgacggcgtc ccctttgccg ggcttgccga
     3781 atttccgggc ggacggctcc aaccgggaga atcctgggag gaggccgctc ggcgggaaac
     3841 cctagaggaa accggcctct ccgttcgcgt cgaggccctc ttgggcaccg tttccactcg
     3901 atacgaggac cgtatcctgg aactgcgttt tttcgccgcc agccctgtat ccccggcggc
     3961 ctcaccgcgg gaaccatttc gctggattcg tcgggacgcc ctccgtgccg attgctttcc
     4021 cccggccaac cggaccatta ttgagcagat ccaaaaagga cacggccaat gatccgggcc
     4081 gcggccaagg gcctcaagac gttccttttc ccaatactta tgtgaaggtt cccgttagca
     4141 gtgaaggatc cttgtcttcg ggcggcccac ctatgttttg cacccaaagc ctatcccaaa
     4201 aagtccccct ctcccaaagg gagagggggt ttttttacga tgggccacga aatatacgcg
     4261 gccagcccgg tagttgcagc acccacccgg cgcggatgcc atgcatatcg catcggactt
     4321 ggatttcgca cggacccgag cgctggttga atcatgggct aggcacggga gcggaaattt
     4381 gggcaggcac aggggcctgc ccctacgggg tttgaatggg tgcgtcaatc gtaggggcaa
     4441 ccccctgtgg ttgccctgtt tgtccgcggt acgcgcgatt tgcgtttcgc cgggtgcgcg
     4501 ggctgctttg actttgtgcg gacccggcag ttgtttgggt tctggggcag gcacaggggc
     4561 ctgcccctac gggtttcaat cggatcatca atcgtagggg caaccccctg tggttgccca
     4621 gtttggccaa agggacactg gggaaggtcg ccgtcgccgg cgaccaaatc atcggacggc
     4681 ggcgaccccg tccctaccgt tcacggaact attccgcaaa gctcaagaag accacttctg
     4741 ccagggggaa gtgattggtg ctggggcgac cgtgtccctc ccacgctaaa agtctatccc
     4801 aaaaactccc cctctcccgc cgggagaggg ttggggtgag ggcaagcgaa cgtcggaatc
     4861 gatcgggaaa accggcagtg attcgagcca ccctcacccc cgacccctct cccgccgaac
     4921 gggcgagggg agattacggg ataggctcta aacggggcgt gccggtaaag tcaccgctac
     4981 cgacaaagat tagccacttt tcgccgtttc tcccgttatc aaaaccgtaa cacagcgcga
     5041 gatttccgcc tgcggacctt gtttcgcaga ccgttgatgc caagacccga taaaagaacg
     5101 tgggcgatca ttgtcatcgc cgtgccatag gcctacacca atgggcctac gctcggcttt
     5161 ttggcgaggg aacaatcatg tctgaaataa agacccatcc agcttccggg gtaaagaagt
     5221 gcatctgtct cggtctatct tgggcgatgg tgattttctc cgcggcgatc tgggatggca
     5281 tcgcgcttgg gcagaccccg gccgtcctaa gttcattcca tgtcgtggat gtgacgaagc
     5341 gaggggatct gcgtccgcct gtaatcactg gactctatta cgacgccgca cgggacattc
     5401 tgatcactgc tggcgatgac cacctaatcc gcattttcaa gcagcccggc gatcagcaac
     5461 ttgccgtgct gaatggccag gggtggatct acggcctggt gctcgatcct acgggaaagt
     5521 atttggcagc cgtggacgat cgtggactgc tcaccgtatg gcaatgggaa acagcccgcc
     5581 agctttatcg cctcccgctg tcaaccgccg cacttcgtac ggttgcgttt catccagggg
     5641 gaaattggct ggcagtgggt ggttttgaac cgcgggtctt catcgtggaa accctcacgg
     5701 gacggcgagt ggcggacctt aacgccgatc gggatgtccg ccaggtggta ttctccagcg
     5761 atggggcttc tgttgtggca gcaggaggct cgggtaagct acggctttgg gatctcccaa
     5821 gcttacggca gcggatggaa attaccgtat cctctcgacg tttgcacgcg gtagcatttt
     5881 ctcccgacgg aagaatcctc gccgctggag gtgaggagcc cgtcgtcagt ctgtggcagg
     5941 tcgattgtga tcccccttcg ttgatcgggc gactcccgct ttcggacggc atcgtcacca
     6001 gcttgacctt ctgtgaaaac ggtcaacagc ttgcagtcgg gacgacacga aatgtcatcc
     6061 acgtctttga ccttgtgacg tccaaggaac tggcccaatt ggagggtcat acgggcacaa
     6121 ttagctgcct gacatggacg ccggaacgct ccttacttag ttccggaagt tttgatacga
     6181 ccgtccgctt ttggcggctg ctacccggga caactgtttc cgagcgtctt cccactgccc
     6241 aggatatcct gcggcaatga ggcgcggctg cattacaaaa caaaaaccga aaggacatca
     6301 taaaaaccac cttgacaagg aggtgatacg tgaaaggttg gaagggacgc cctcaacaag
     6361 cgccgtcgca aaagcgccgg gatcgccatc cctcgcgtta ccgaaagtgc cggctggaac
     6421 agatggaacc aaggatgctc ctctcggtat cacccatcca gttggggatg gtctattacg
     6481 aggatgccac cggcaccgat gaaagcggcg atacgtttga gctgacgttc caagggggcg
     6541 cgccgggcac gcagttgaca cggttggtga tcaacaccga taagctggga gatggcttgt
     6601 ccatcgggga ctgctttttt gacaccgaac ccggcgggct gggggccttt ggttacggtc
     6661 cctttgcggt gatctcggcc agcggcattg acaattttcg ggtaagcgta tcagacggcg
     6721 gtaccgaact cgtcctggaa ttcacaggct ttgaggcggg cgaccgcttc attttcacca
     6781 tcgacgtgga cgaaatgggg ttcctcggtc ctaacgcggt tgccgaaggc aatgagtttg
     6841 aaggctcacg tttaacggcc tatttcgtgg ctccgcacta ttatgcggcc cagggtgaag
     6901 acatcttcat tgacgcttac gaccacaagt tatccgcttc ggggctggtc cttccccacg
     6961 attcctatag cccgccgttg ccggaacctg aacccgtccg cacggccggg gcctttctgg
     7021 aagtgactca gacgcctttg ccggttgcca tctccggccg ggtgttcgaa gattttaacc
     7081 tcgatctcac cctcaacgga gaggacaggc ctttggccgg tgttaccctc gaattgtggc
     7141 agtggcaagg cgagacctat atcttcacgg gacgaacgac cgtcacggat gccgacggca
     7201 gctaccgctt tgacgatctc ctccccggta cctaccgcat tgtggagcgt cagcccgaag
     7261 gttttctcag cgtcggcgcc aaggcaggga gtgtcggttc ggaggtccgg ggttcggtgc
     7321 tgacctctga tatcatcgtg ggaatttcgc tcttaggtgg tgaggagagc atcgagaata
     7381 attttgccga ggttcggcct gcggagcttt ccggccacgt ttatcacgac gttaacaaca
     7441 acgggctact ggagtccggc gaaacgggaa tcggtggcgt caccgtgatt gtcatccgcg
     7501 aaggtgatca ctttctgacc gagcaagagt tcaccgtcat cacccagacc gacggaagct
     7561 ggtccgttgt ggatctctat ccggggaaat accgcgtgca agaaatccag cccgccggtt
     7621 ggcttgacgg tctcgatcgc gccggctcgc tcggtggctc ggccgtcaat cccggtgatc
     7681 agattgtcaa catcaaagtc ccggcgggaa gccaaggggt taactatgac ttcggcgagc
     7741 ttctaccagc ctcgcttgcc ggcctggtgt atctggacct aaatacgaac tgccaatatg
     7801 acccaagcga gccggtcctc cctggggtga ccattaagct gctgtcgccc gacggtactg
     7861 tcctccggac aaccgtgaca gatgcctctg gccggtatgt ctttgagaac ctcacgccgg
     7921 gcacttacgc cctccatgaa atccaacctg agggctactt tgacggctgt gatcgaccag
     7981 gcacggccgg cggcgtcaag tccccgccgg atttgatcac ggatattcac ctcgcgtcgg
     8041 gcacggccgc cactgattac ttatttggag aaatcccccc cgcatcggtc gccggtttgg
     8101 tgttttccga ccttaacgcc aacgatcaac ttgatgccga ggatgccccg attgccgggg
     8161 tgaccatcca actgttcgct gccaatggtg aactggttgc caccacggtg accgacgccg
     8221 agggacgcta ccggtttgag aacctgcgcc ccggttatta caccatcgtc gaggttcaac
     8281 cgaatggcta cctggacggt gccgatttcg tggggagcgc cggcggtcaa cgcgacggca
     8341 gtgaccgcat cacggacatc aagcttcctc ccggtgtcca cgcaacggat tacaactttg
     8401 cggagatcct gcccgccacg atcagcggtt acgtctttca ggatggtccc accattacct
     8461 acgtgtgggg tcaggagcgt ccggatccat acctcgttcg tgatggccaa ttcaccccag
     8521 atgatattcc cattagcgga gtcgaaatcc agttgcggga tgccgttggg caaccaatcg
     8581 tgggtgaaga tggtcggccg cttgtgacct ggacggacga acgagggtat tacgagtttc
     8641 gcgggttgag gcccggcgtt tacacccttt tggaaatcca tccggcaggc tacgaggacg
     8701 gcttggacac accgggcacg ctgggaggga ttgcggtcaa ccccaatgcc tcgttggatc
     8761 ctgccatcct tgccgccctg accatcgacc cgcggaatga tgcgattctc ctggtaactg
     8821 tgacaccggg tgccgttgga acaaattaca attttgccga ggtcgttgtg acctccgttc
     8881 cgccctatgt gccgccgact ccgcccccgc cgtggtcgcc accggaacgg ccggcctcgc
     8941 ctggggttcc tcctgggaca ccaccgattc acctgcaaat ttccccggtg atgcccgaat
     9001 cggtggccct gccgcgattc ggaggaagtg ccggaacggt agactacacc tggcatttga
     9061 gcgtgatcaa tggtggtgtt cctcggcaca atctcggcgg ataccaacgg gtcttgatcc
     9121 gagaggtcag cctattcaat ccggcgcggt gggaggggat tccgctcgat cgcggccaat
     9181 ggacaatcgt tgacatcatt actggggaaa ctacggccct tggcaatcta ggtaccgagg
     9241 ccgctttgcc cgtggtggga gatttcagcg gcatcggtcg cgtacagctc ggcatcttct
     9301 acggggggat gttcttcatc gacctcaatg gtaacggcat ttgggacgac caagacctct
     9361 ggattcagct tggcaacgac cgtgatcagc cggtagccgg ggactgggat ggcgacggaa
     9421 aatgggatgt cggcatcttt gggcccgctt ggcccggtga tgaccgggcc ctgcgggcgg
     9481 aaccgggctt gcccgacgcc caaaaccgcc tgacggcctt tcgtccacga aacctgccac
     9541 ccactccaga ccaggcaccg gacgatcaac gcctcctcca gcaagggcga aacggaccca
     9601 cccgggctga cctcattgac cacgtgttca tgtacggtca accgggcgac cacgccgtgg
     9661 tgggcgactg gaacggcgat ggtatcgcca ccgttggcgt cttccgcggc gggacgtggt
     9721 atctcgatga agatggcgat ggtcgctggg gaagccgcga tcgggtagcc ttcttcggcc
     9781 aggccggcga tctccctgtc gtcggagatt ggaatggcga cggaatcacc gacctcgggg
     9841 tgtatcgtga tggccgattc tatctcgata tcgacggcaa tggccaactg acagccgccg
     9901 acaaagtcat caccttagga cagcctggtg atcgcccctt cgccggtgat ttcaacgggg
     9961 acggtcgcgc tgaagttggc gtgtaccgca cccagagccc tagcactggg ggaggccaaa
    10021 cccttcggga ggcagccgct ccccgtggtg ttgccaaatg acgcgtgacg agaatacccc
    10081 attaatgggg ggcatccttt ccaattttac ccaaaacggc gtcttccccg tgaagacgcc
    10141 ctatttagaa cgtatcccaa aatctcccct ctcccgttcg gcgggagagg ggtcgggggt
    10201 gagggtggct cgaatcgctg cc
//
LOCUS       DAOPTR010000012         9532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_6017_length_9532_cov_3.229186, whole genome shotgun sequence.
ACCESSION   DAOPTR010000012 DAOPTR010000000
VERSION     DAOPTR010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 9532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9532
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6017_length_9532_cov_3.229186"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PKI05_00505"
     CDS             complement(<1..560)
                     /locus_tag="PKI05_00505"
                     /inference="COORDINATES: protein motif:HMM:NF014097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HOA50703.1"
                     /translation="MTVYSPTYNTPEHRPSSPPRLRPRREDRKARHRLEILNVLRKTN
                     RPLTSSQIAEQLASAGYILSERSIRLYLNELDAEGLTISAGHRGHLISDQGLAELRVA
                     QTIQRVGYLSARIDEMTYKMTFDLERRTGTVVVNTSIINPHDLAQCLDSVCQVFYKGY
                     AMGTRLAILEPGQRMGEVEIPPGRIG"
     gene            1005..2642
                     /locus_tag="PKI05_00510"
     CDS             1005..2642
                     /locus_tag="PKI05_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOA50704.1"
                     /translation="MQYRKQNDVLGTVNRGNPADSGLCTLCEVECKGKCETWLSSMLG
                     RKMLYPRNFGNVTAGSANVVAIGVGYHALRIQGYAYDSTGLPPGVGDSPDDCVFPKVD
                     VRTEFGREVKTKCRVPLMTGALGSTFVAAKYWDSLAIGAALCGFPIVIGENVVGVDPK
                     SVFKNGRVVESPELDRRIQSFLRYYDGHGAIIVQMNVEDSRNGVAQYLSEKYGDQVIL
                     ELKWGQGAKDIGGEIQVTDIEYARFLKDRGYLIDPDPYDPTTEAAYRKGAIRSFARHS
                     RLGASNFHSAEQVYEDFLNSIAFFRSLGFKRITLKTGAYGMEALALALRCAADAKLDL
                     LTVDGAGGGTGMSPWNMMEYWGIPSLPLHSKTYEYCQILKQRGIQPPDISLGGGLGRE
                     DHIFKALAICAPFTKLVCMGRAMMIPAFLGSNIEGVLRPERRKQVNGNWDSLPPSVAE
                     IGTKPEEIFVAWETVKNKVGTEEMKNIPFGAVAVCCLVDKLIAGLQQLMAGARRFSLP
                     AIRREDLMAANRETAEVTGIPYMLDAQNDLALAILKK"
     gene            3045..3542
                     /locus_tag="PKI05_00515"
     CDS             3045..3542
                     /locus_tag="PKI05_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50705.1"
                     /translation="MITANYRKLAKAAVVGLVLCCGWTIASLAWGQEGLSSSPPESAS
                     AAAAGSTLATEQPCPVGDPCHCLCHHGHQGCLARYMSAVGGFNCSCRGSYKFPVPPQY
                     TYHWPGLYSQQLMTSYQSPYRYPLLKLPPWMEKRPNADVPQNGALMPLMPSPGEKHAP
                     SNSGG"
     gene            3666..6464
                     /locus_tag="PKI05_00520"
     CDS             3666..6464
                     /locus_tag="PKI05_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HOA50706.1"
                     /translation="MTRMKWLQEAQPLTVIRWAAFACAVIFSLGLAYGLWQTTGHFFV
                     QANEPGGQSSVPIRLEVGTVQPLSNAAPSSQPSAEEPLVKLSIQPTIKREINNAPRPA
                     QNGSSAPTSKPNDSKPNDQDEIWIESDKPQEQSAASNKAMGEIWVEEDQPGNSLPAVA
                     AVPTPEKPSKDLVEARPATASPQEQCRIVLGNSESTVLRIAKAHVVRPSVSESASNQP
                     VAKEPPVPSPWAGSLVLPENSPDVLRKSREITELYQTFTIGGTAIFVKRAESKRPLSS
                     GEVPLSPEPAAHDEVTLTSAEAQVGQGTKEEKEPAREAVVVGWEFPGQTTESPKVDST
                     TLAITSAKPVFSQRDRLAQRDVHWETMPIQRPIGLASPQPDGQEQDDQKSSSGVVGSS
                     RTPSSAATQETENKDRRVQPLVTSQNDLRSENTLVKVSEFSNQSVPSPRATLASQENQ
                     PQSNQNLVEVVPNESRGEEPSFEIIEESGELTVVVRRSKLLRTKVDVYRTAVVDSGIC
                     DVVQFTPREISILGKSQGATHVTFWFQDERYRPVTYLVRVTPDPEVEKQQTERYELLE
                     SHLAKLFPESKVDLVVVGDKLVVRGQARDAAEAAQIMAIIRGELVRARGVMEGQASDP
                     VGREAAGQALPAIQVINMLRIPGPPQVALKVKIAELNRTAARNFGVDMSLNFDQGKFL
                     IQSLLNLSSGGSASILGNFDGGDLKFGLHYLEEHGVVRLLSEPTLITLSGRPATFIAG
                     GEFAVPTTVGVNGVGAVTTDFRAYGAIITFLPTVLDKDLIRLEVAPEFSQLDTKNKVN
                     GTPGLRTRAVTTTVEMREGQTLAIAGLLDDSMTGNNQGDLPIFNQIFGRRSLSRNETE
                     LIILVTPELVHPMEPEEVPPLPGFDVTEPTNHEFYIKGHLEGRPTQEYDSTTWPRLRR
                     RYNAGGPARISGPFGHGE"
     gene            6541..7188
                     /locus_tag="PKI05_00525"
     CDS             6541..7188
                     /locus_tag="PKI05_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50707.1"
                     /translation="MDGYQRLRHRGLSGSLPSMDAAGLASLSARPTWLSRGRWATGLT
                     LAIALVVTGCHTPKVSHEAKMGPVYDRPFPLGQVTDAHWETMQTNAEAADFIFYDHEF
                     VGETAELTPLGRKHLLSVALRLEHVPFPIVVEESPRGENPQLDESRRQTIINQLAQLG
                     LDRTMVEARTVVAPAFTEGLSAIEGEAAYYRTLMDGNYGVGSYGSLGRGFGGWRR"
     gene            complement(7279..7767)
                     /locus_tag="PKI05_00530"
     CDS             complement(7279..7767)
                     /locus_tag="PKI05_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50708.1"
                     /translation="MTDFEKRLQKAIERGQQIRKQRAEAEIRRALTEQELRTRHSDLR
                     LQLTEYIEQCLKKLIEHLPGFTLESVFSERGWGSAVSRDELQWRRGESPQQRFSRLEL
                     VVRPFSEYYVLDLVGKATVRDKELFHRSFYQPLAEADLSAFKETVDRWVLEFAEAYTA
                     RG"
     gene            complement(7962..9422)
                     /locus_tag="PKI05_00535"
     CDS             complement(7962..9422)
                     /locus_tag="PKI05_00535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOA50709.1"
                     /translation="MLKRLRRWWLRPCGARTVLTVAMPLVISFASWTVMNFTDRMFLV
                     WYDIGAVAAVLPAGMVQWTLAAFAMGLVSYVTTFVAQYQGAKRYHRVGPVLGQAYWTA
                     LLFIPPLMATIPFAYEMFSWIGHPPELAELEARYYQVAMLAVGAMLFSSVQAGFFTGR
                     GDTRTVMIVDTGAALLNLVLDYLWIFGHLGFPEGGVEGAAWASTVAQWAKVVWYGVLM
                     SGSRYDEYCLRDAWRFEGPLFRRLWRFGGPNGLQWLLEGAGFTGFLLLVGRLGEMATA
                     ATNLAINVNSVAFVPAIGLSIAVTTLVGQELGANRPRRAAVATWTAFHLAVIYTAVLA
                     IIYVAAPDVFLVAYGAGHDPAEFAPLRELVIVLLRFVAIYCIFDAMNLVFSGALRGAG
                     DTRFILLVGLVMSPIPTVLIWWGITSWSAGITFSWVVITLWTCALGVIYLVRFLQGHW
                     RTIRVIEQTVIDEAESISELANLPVKAPAGILSEAD"
BASE COUNT         2225 a         2741 c         2636 g         1930 t
ORIGIN      
        1 aagccgatac ggcccggcgg aatctccacc tcacccatcc gctggccagg ctctagaatc
       61 gccagccgcg ttcccatggc ataccccttg tagaagacct ggcacacgct gtcgagacat
      121 tgcgcaagat catgcggatt gatgatcgat gtgttcacca cgacggtgcc cgtgcgacgc
      181 tccaggtcaa acgtcatctt gtaggtcatc tcgtcgatgc gggcggagag ataacccacg
      241 cgctggatgg tctgggcgac tcgcagttcg gctagtcctt gatcagagat caaatggcca
      301 cgatggccag ccgaaatcgt caacccctcg gcgtcaagtt cattcagata aaggcgaatg
      361 ctccgctcac tcaagatata gccggccgaa gccaactgct ctgctatttg cgaggacgtc
      421 aagggacgat tggtttttct caagacgttc agaatttcca gccgatggcg ggcctttcgg
      481 tcctcacgcc gcggacgaag ccgtggtggg gacgagggac gatgctcggg cgtgttatag
      541 gtcggtgaat acacggtcat gtcgcgtctt tcctcaatac cgttttgagt agcaccctac
      601 atttgcctgc gggatcaaca cgatccaaaa tcccgaaact tgccgcgatt tgccacagcc
      661 ttcggtaaca gcggaaatgg agcttggcag cggtctccaa aaatgtttcc gcaagcaccg
      721 actatctggc aagaaagctc cgaaggccag accagccttt tcctattcct ccaatgttat
      781 ggcaatttta ccggatttcg tcggcactgt caatgccgcg gccattttgg gcatcttgca
      841 aaaatcagcg tgtagcacgg ttaaccctat agacaaccgc ccccagcgac gctaaactac
      901 cacgaagtct aattggcagt gacctgccag atctcggaag gcgctccttt ccagcccgcg
      961 gtcaaattac tgtctcgtgt acggtccttt caggagtgtg aggtatgcag tatcggaagc
     1021 agaatgacgt tctaggcaca gttaatcggg ggaacccggc ggattcgggc ctttgtaccc
     1081 tctgcgaagt cgagtgcaaa ggaaagtgtg aaacgtggct gagctcgatg cttgggcgaa
     1141 aaatgcttta tccgcggaat tttggtaacg taaccgcggg ttcagccaat gtggtcgcga
     1201 ttggggtcgg ctatcacgcc ctgcggattc agggctatgc ctacgactcc actggtttgc
     1261 cgccgggagt gggggattca ccggatgatt gcgtttttcc caaggttgac gtccgcaccg
     1321 aatttgggcg agaagttaag acaaagtgtc gtgttccttt aatgacaggg gccttggggt
     1381 caacgtttgt cgccgcaaaa tactgggatt cactggccat cggggcagcc ctgtgcggtt
     1441 ttccgatcgt gattggggaa aacgttgtag gcgttgatcc caagtcagtg ttcaagaacg
     1501 gtcgcgtggt ggagtcgccg gaacttgatc gccgcatcca atcgtttctt cgctactatg
     1561 atggtcacgg cgccattatc gtccaaatga atgtcgagga tagccgaaat ggagtggctc
     1621 agtacctcag cgagaaatat ggtgatcaag tcattctgga gttgaaatgg ggacagggcg
     1681 cgaaagatat cgggggtgaa atccaggtga cggatatcga atatgcccgc tttctgaaag
     1741 accgcggcta ccttatcgat ccagacccct acgaccccac cactgaagcc gcgtatcgga
     1801 aaggggccat acgctccttt gcacgccaca gccgtctagg tgcatcgaac ttccattctg
     1861 cagagcaagt ttacgaagat ttcctcaact ccatcgcctt tttccgcagc ctgggattta
     1921 agcggattac cttaaagacg ggcgcctacg ggatggaggc ccttgccttg gccctccgtt
     1981 gcgctgccga tgccaaactt gatctgctca ccgtcgatgg cgccggcgga ggaacaggga
     2041 tgagcccatg gaacatgatg gaatactggg gtatcccctc cttgccgctc cacagtaaga
     2101 cctacgagta ctgccaaatc cttaaacaac gtggtatcca acctcccgac atttcgcttg
     2161 gtggcgggct cggccgagaa gaccacatct tcaaggccct cgccatttgc gcaccgttta
     2221 cgaaacttgt gtgcatgggc cgcgccatga tgataccggc gtttttggga agcaatatcg
     2281 aaggcgtcct gcggcccgag cgtcgcaagc aagtcaacgg caattgggac agtctgccgc
     2341 ctagcgtcgc cgaaatcggc acaaaacccg aggaaatctt cgtagcttgg gaaaccgtga
     2401 aaaataaggt cggtacagaa gagatgaaga acatcccgtt tggggccgtg gccgtttgct
     2461 gcctcgttga taaactcatc gcaggcttgc aacaactcat ggccggggca cggcggttca
     2521 gtcttccggc catccgacgc gaggatttga tggcagccaa tcgtgaaacg gccgaggtaa
     2581 cgggaatacc ttacatgctc gacgcacaaa acgacctggc cctcgccatt ctgaaaaagt
     2641 aacctttgcc taacttttag ggatcacgtt tcgcccggtg ataggctcgg cccgtcaccg
     2701 ggtttttgtt tggtcacaaa cgtttcataa cattgcgatg cccgaagcaa ttgcgatcac
     2761 aacgccgcac gctggtagga gcaactctta tgcccgcttt accttctcca acgagcattc
     2821 catcatgaaa ggcggttcgg cgggtggcta tccgcacgga aatctaggca agatatgccg
     2881 aatgtgccga cccgatccag cagttttgga attacgccaa catgcgttac agaaatcgct
     2941 gaacgccttc acgttttttt gacgatagtc gttaccagtc acacagttcg tacagcagtc
     3001 agaatcgtcc ccgctagtgg gggatttcac ccccggaggt ggccgtgatc accgctaatt
     3061 atcgaaagct tgccaaggcg gcggttgtgg ggcttgtgtt gtgttgtggt tggactatcg
     3121 ccagtctggc ttggggtcaa gagggccttt cgtcctctcc cccggagagc gcaagtgcgg
     3181 ccgccgccgg ttcgaccttg gccaccgagc aaccttgccc ggttggcgac ccttgtcatt
     3241 gtctttgtca tcacggccat cagggctgtt tggcacgata tatgtcggcg gtgggtggct
     3301 tcaattgcag ttgtcgggga agttataagt tccccgttcc cccgcaatac acctaccact
     3361 ggccgggtct gtacagccaa cagttgatga catcctacca aagcccatat cgctatcctt
     3421 tgctcaagtt acccccgtgg atggaaaaaa ggccgaacgc ggacgtccct caaaacggag
     3481 cgttaatgcc attgatgccc tcacccgggg agaagcacgc tccgagtaac tcgggcgggt
     3541 agtttttgcg agaagacagc acaagggagt atgacgctga gtcggtaagt cgaaaccgat
     3601 tccaccagag gagagtcaac tttctctgac caaggtcggt caggtttgat atcaaggatg
     3661 agaccatgac acggatgaag tggctccaag aggctcagcc gttaacggtg attcgctggg
     3721 cagcgttcgc ttgcgcggtg atcttttcgc taggattggc ttatggcttg tggcagacca
     3781 ccggtcattt tttcgttcaa gcaaacgaac ccggagggca aagtagcgtt cctatccgtc
     3841 ttgaggtggg aaccgtccag ccgttaagta acgcagcacc ttcgagtcag ccatcggcag
     3901 aggaaccgct cgtcaaattg tcgattcaac cgaccatcaa gagagaaatc aacaacgcac
     3961 cacgcccagc gcaaaacggc tcatcggccc caacgagcaa gccaaatgac agcaagccaa
     4021 atgaccagga tgaaatctgg atcgagtcgg ataaacctca agaacagtcg gcagcctcga
     4081 acaaagcgat gggcgagatt tgggtcgaag aagaccaacc gggcaacagc ctcccagccg
     4141 ttgccgcggt accaactccc gaaaagccaa gcaaggactt ggtggaagcc cgtcccgcca
     4201 ctgcctcccc ccaggaacag tgccgcattg tccttggaaa ttcagagagc accgtattac
     4261 gaatcgccaa ggcccatgtg gttcgtccgt ccgtcagtga atcggcctca aatcagccgg
     4321 tggcgaaaga accccctgtt cccagcccat gggcaggcag ccttgtttta cccgaaaact
     4381 cgcccgatgt cctccgcaag agccgggaga tcactgagct atatcaaact ttcaccattg
     4441 gcgggacagc tatctttgta aagcgggctg aatcgaagcg gcccctttcg tccggcgaag
     4501 ttcccctgtc cccggaacct gcggcccacg atgaggtgac attaacttcg gccgaggcgc
     4561 aagtcgggca ggggacaaaa gaagaaaagg aacccgcccg tgaggccgtg gtcgtcggat
     4621 gggaattccc ggggcaaaca actgagtctc ccaaagtcga ttccacgacg ctggccatca
     4681 cgtccgccaa gcccgtgttt tctcagcggg atcggctggc ccagcgcgat gttcattggg
     4741 agacaatgcc tatccaaagg cctatcgggc ttgccagtcc gcagcccgac gggcaggagc
     4801 aagacgatca gaagtcctcg tcgggggttg taggctcaag tcgcacaccg tcttcggcag
     4861 cgactcaaga gactgaaaac aaggaccgcc gcgttcagcc gctggtgacc tcccaaaatg
     4921 acttgagatc cgagaacacg ctcgtaaaag tcagtgagtt ttccaatcag tcggtgccat
     4981 cgccgcgggc aaccttggcc agtcaagaaa accaaccgca gtccaaccag aatctggtgg
     5041 aggttgttcc caatgaatct cgcggggagg aaccatcctt tgaaattatc gaggaatccg
     5101 gtgagctgac ggtcgttgtc cgtcgcagca aactcctacg gacaaaagtg gacgtttacc
     5161 gcacggctgt ggtggactcc ggtatctgtg atgtggtcca gttcaccccg cgggaaatct
     5221 cgattttagg caaaagccag ggggctactc atgtgacgtt ctggttccaa gacgagcgct
     5281 atcggccggt cacgtacctt gtgcgagtta cccccgaccc tgaagtcgaa aaacagcaga
     5341 ccgagcgtta cgaattgctg gagtcacacc tggcgaagct tttccccgaa agcaaggtcg
     5401 atttggtggt cgtcggcgac aagctggtcg ttcgcggcca agcccgtgat gccgccgagg
     5461 cggcccagat catggccatt attcgtggag agcttgtgag ggcacggggc gtgatggagg
     5521 gacaggcttc ggacccggta ggacgggaag cggctggtca ggccttaccg gcgattcagg
     5581 tgatcaatat gttacggatc cccggtccgc cacaggtggc actgaaggtt aagattgcgg
     5641 agttgaatcg cacggccgcc cgtaacttcg gtgtcgatat gagcctgaat ttcgatcaag
     5701 ggaagtttct cattcagtcg ttactaaacc tttcgtccgg cggcagtgcc tcgatccttg
     5761 ggaacttcga tggaggggat ctaaagttcg gcctgcacta tctggaagaa cacggcgtgg
     5821 tacgcctgct cagcgagcca acgttgatta ccctaagtgg ccgcccggct accttcatcg
     5881 ccggtggcga gtttgccgtg cccacaaccg tgggcgtgaa tggggtggga gcggttacga
     5941 ccgacttccg tgcctatggt gccatcatta cgttcctgcc tacggtgctc gacaaagacc
     6001 taatccggtt ggaagtggca cccgagttca gccagcttga tacaaaaaac aaggtcaacg
     6061 gcacaccggg cttgcggacg cgggccgtga ccacaaccgt cgaaatgcga gaaggacaaa
     6121 ccttggccat cgctggtttg ctcgacgatt ctatgaccgg caataaccag ggcgacctgc
     6181 ccatcttcaa ccaaatcttt ggacgacgaa gtctgagccg caacgagacg gagctcataa
     6241 tccttgtgac acctgaattg gttcacccca tggagccgga agaagtcccg ccgttgccag
     6301 gtttcgacgt aacagagccg accaatcacg agttctacat caagggtcac ctcgaaggac
     6361 gcccaacgca ggaatacgac agcaccacct ggccacggtt acggcgacgc tacaacgcgg
     6421 gtggtccggc caggatttcc gggccgttcg gtcacgggga gtaaaagagt ctcacacgct
     6481 taggaataga tcaaatcatc gcgaaagatc atcaagcaac tcacattcgg gacaattgcc
     6541 atggatggtt atcaacgttt gcgtcaccga ggacttagtg gtagcctgcc cagtatggat
     6601 gctgcgggat tggccagcct gtcggccaga cccacatggc tgtcgcgtgg gagatgggcg
     6661 acgggtctca ctcttgccat tgccctggtc gtgacaggtt gccatacgcc caaggtctcc
     6721 cacgaagcga agatgggacc ggtttacgac cggccgtttc cactcgggca agtgaccgac
     6781 gcacattggg agaccatgca aaccaacgcc gaggccgccg atttcatctt ctacgaccac
     6841 gagttcgtgg gagagacggc ggaattgacg cccttgggtc gtaagcacct cctgtcagtt
     6901 gcccttcgcc tcgaacacgt gcctttcccc attgtggtgg aagaatcgcc acgcggtgaa
     6961 aatccccaat tggacgagag tcgtcggcag acgattatca accaattggc ccaattaggc
     7021 cttgatcgca ccatggtcga ggcccgcacc gttgtggcac ccgccttcac ggaaggattg
     7081 tcagccatcg aaggtgaggc ggcctattac cggacactca tggacggtaa ttacggcgtt
     7141 ggttcttacg gcagcctcgg ccggggcttc ggcggctggc ggcggtaagg catcacgcag
     7201 aggaacgccg aaaaggcagc ttacttcgat ctaatgaaga cgtcacagtt cgacctaacg
     7261 gcctggtgac ctggcagttc acccacgggc cgtgtaggcc tctgcgaact ccagaaccca
     7321 gcgatccacc gtttctttaa aagcggacag gtcggcctcg gccagcggtt gatagaagct
     7381 gcgatgaaaa agctctttat cacggacggt ggcttttccc accagatcga gaacgtagta
     7441 ctccgagaat ggccggacta ccagttctag ccggctaaag cgctgttggg ggctttcacc
     7501 ccgtcgccat tgtaattcat cccgactgac ggcactcccc cagccccttt ctgaaaatac
     7561 cgattccaat gtgaagccag gcagatgctc gatcagcttc ttgaggcact gctcaatata
     7621 ctccgtcagt tgcagccgca gatcgctatg acgtgttcga agctcctgct cagtcagggc
     7681 gcgccggatc tcggcctccg cacgttgctt gcggatttgt tgaccacgtt caatggcttt
     7741 ttgcaatcgt ttttcgaaat cggtcatggg acgataaata cctcggcgcg accgttccat
     7801 ttcaaggatg agccacgatc ccggctactt aggcgacaac cccatcaatt ttatcataac
     7861 tggtccgggg aatgctgcct ggcgtcggta agaagccaag tgctccaact cgaaactttc
     7921 aaatgccttt ggggtgtccc aatacacctg cccccaaggc attaatctgc ttcgctcaaa
     7981 ataccggcgg gagccttgac aggcaaattg gccagctcgg aaattgactc cgcttcgtcg
     8041 atcacggtct gttcaatgac ccgaattgtc cgccagtggc cctggaggaa tcgcacgagg
     8101 taaattacgc ctagggcaca ggtccacaag gtgatgacaa cccacgagaa ggtgatcccg
     8161 gcgctccagg acgtgattcc ccaccagatc aaaaccgttg gaataggcga catcaccaga
     8221 cccacaagca gaataaaccg cgtatctccg gcaccccgca acgccccgga aaagacgaga
     8281 ttcatcgcgt caaagatgca gtaaatcgcc acaaagcgga gtagaacgat caccaattcc
     8341 cgtaatgggg caaactcggc gggatcgtgt ccggcgccgt aggcaacaag gaagacatcg
     8401 ggcgcagcca cgtagataat cgctaaaacc gctgtgtaga tcacagccag gtgaaaggcc
     8461 gtccaagtgg ctaccgcagc ccgccgcggt cgattggccc ccaactcctg accaaccaac
     8521 gtcgtcacgg caattgacag cccgatagcg ggcacaaagg ccacactgtt gacattgatc
     8581 gccagatttg tggccgccgt ggccatttca ccgagccgtc ccacgagaag caaaaatccg
     8641 gtgaaaccgg ccccctctaa aagccactgt agcccattcg gcccgccaaa ccgccaaaga
     8701 cgacggaaaa ggggcccttc aaaccgccag gcatcgcgca ggcagtattc atcgtaccta
     8761 ctgccagaca tcagaacccc gtaccacacg actttggccc attgggcaac ggtgctggcc
     8821 caggccgctc cttccactcc tccctcggga aagcccagat gaccgaaaat ccaaagatag
     8881 tccaggacaa gattcaataa ggcggcgcct gtatcgacga tcatgaccgt ccgggtgtcg
     8941 ccgcgtcccg tgaaaaagcc ggcctggacg gacgaaaaga gcatcgcccc cacggcgagc
     9001 atagcgacct gataatatcg agcctcaagc tccgccaact ccggcggatg gccaatccac
     9061 gagaacatct cgtacgcaaa aggaatcgtc gccatgagag gcggaatgaa tagtaaggcc
     9121 gtccagtagg cctgccccag gaccggcccc acccggtggt aacgtttggc cccctgatac
     9181 tgagccacga aggtggtgac gtagctcacc aaacccattg cgaaggcggc caacgtccat
     9241 tggaccattc ctgcgggcag gacggccgcg acagccccga tgtcgtacca aacgagaaac
     9301 atccggtcgg tgaaattcat caccgtccac gaggcgaatg agatcacaag cggcatggcg
     9361 acggtgagga ccgtgcgggc accacacggg cgtagccacc atcgacgcag tcgcttcagc
     9421 atagagaccc tcctacgtga tgcaatttca acagtcggca actccgtgat aacccgcccc
     9481 ggtaactggt ttccaacctc ctaagccctg ccccaaatct gaccccagcc ca
//
LOCUS       DAOPTR010000013         9395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_6127_length_9395_cov_3.691113, whole genome shotgun sequence.
ACCESSION   DAOPTR010000013 DAOPTR010000000
VERSION     DAOPTR010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 9395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9395
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6127_length_9395_cov_3.691113"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /gene="glmS"
                     /locus_tag="PKI05_00540"
     CDS             <1..1025
                     /gene="glmS"
                     /locus_tag="PKI05_00540"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOA50710.1"
                     /translation="GRIDLDVASAKFGGLNLSPQQLRQVERIVLTGCGTSWHAGLVGE
                     YQIEAIARIPVEVEYASELRYRNPPLHRNTILFAITQSGETADTLAALRELKRKGYPT
                     LAICNVVGSTIAQEADGGIYLHAGPEIGVASTKAFTSQCLVLALLALYFGRLRDLSFE
                     AGTRIIEKLRELPNLVARALECDEKVRRIADRFYQCRNFLYLGRQFNFPTALEGALKL
                     KEISYIHAEGYPAAEMKHGPIALVDEQTPSVFLLPQGHVYEKVLANMQETKARGGPII
                     AVVEEGDQEAWRIADEIIEVPAVDDFLQPIVFIIPLQLLAYHIAVARGCDVDKPRNLA
                     KSVTVE"
     gene            complement(1049..1618)
                     /locus_tag="PKI05_00545"
     CDS             complement(1049..1618)
                     /locus_tag="PKI05_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50711.1"
                     /translation="MAKKKHRDNFEIDLLLRNVELRTELEPYYDDSLTRVNIHQWPLA
                     AENEYLASMLAWEMAPVLPIYRWFDPELRLPRPETLSDEQLHAILWDTIHKLYQKRIV
                     LEFTDHLSDRELYCLIYRDILPSREKKVDLGNNYLRFDCAFVEGDPEVWLRYYATDAE
                     REAWATTYQQPLPPKEKPPYPRVLPKAPA"
     gene            complement(1996..2505)
                     /gene="rpiB"
                     /locus_tag="PKI05_00550"
     CDS             complement(1996..2505)
                     /gene="rpiB"
                     /locus_tag="PKI05_00550"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOA50712.1"
                     /translation="MRIAIGSDHRGYSIRPKIVAFLKNMGHEVIDVGTFSQSPVDYPD
                     VAEAVARKVSSGEVDRGILFCGTGLGMCIAANKIQGIRAVPCHDDLTAEMSRRHNDAN
                     ILCLSADLLGERLITRIVEVWLNTPFEGGRHARRVEKIAAIEKELKEKCCCPDPQQSF
                     SEENDTKAQ"
     gene            complement(2598..3749)
                     /locus_tag="PKI05_00555"
     CDS             complement(2598..3749)
                     /locus_tag="PKI05_00555"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOA50713.1"
                     /translation="MDSPLVIPLRQAEEPRDLVHRCVQALAEGRLVALPTETIYGVAA
                     SVLVPEAVERLAALKGRKAGHPFTLAIKSGEELWDYVPNLNRVGWRLAQRAWPGPMTL
                     VLKAEGGRSVVERFAPCVRELVSPEGSIGFRVPKHPFVAEVLRLLPAPLVLTSVNRSG
                     EAPATTAEEVVKRLPGLDIVFDDGPSQLGVASTVVRVTEDGWEILRQGSLSEEYVRRM
                     AAAVILFVCTGNTCRSPMAEAICRHMLAEKLGCSSEDLEWRGVVVSSAGLEAYCGDVP
                     SPHAVQIMHERGFDISNHVSRPLTERFVREADCVFAMTKAIRNAIVAQWPEAADRVFL
                     LHPEGKDICDPFGATVETYRMSAGMIEEALLRRCDELLALIGSGKAPHT"
     gene            complement(3892..5019)
                     /gene="queG"
                     /locus_tag="PKI05_00560"
     CDS             complement(3892..5019)
                     /gene="queG"
                     /locus_tag="PKI05_00560"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOA50714.1"
                     /translation="MPLNIVIRLKDLAKDLGFCCVGICPAVEPPHYGAFLKWLEAGYH
                     GEMHYLAQRKEAYRSPSSILPGARSLIMLGFPYQRDAEQNPPAGYGLIARYANGPDYH
                     KTIRQWLRKLGRFLQAEYPSAAVRGVVDTAPLLERDFAQLAGLGWIGKNTLLIGPDCG
                     SFTFLAALLTTAELDFDPPFGGDRCGSCSACLRACPTGALVSPRVLDARRCLSYLTIE
                     LRGFIPRELRELVGNWFFGCDLCQEVCPWNRRLGPTAQATSEATETRVINLIALFRLK
                     EDEFRKMFGPTALWRAKRRGLLRNAAIVLGNQRNQAAWDVLVSGLEDSEPVVREACAW
                     ALARIDIAAAGPIIRAHLAKETDPAVAASLSGYLAETSSSG"
     gene            5284..5517
                     /locus_tag="PKI05_00565"
     CDS             5284..5517
                     /locus_tag="PKI05_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50715.1"
                     /translation="MRHTKLRINPTIWALSAIGLLGLFSFAGCQSRPPLEELGELEFA
                     VPDIPGIDDPYPFPDVAGSAEKPADSEQSDDEP"
     gene            5668..6819
                     /locus_tag="PKI05_00570"
     CDS             5668..6819
                     /locus_tag="PKI05_00570"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HOA50716.1"
                     /translation="MLWRYLMSCACGVVVFLAMDGQKLMGEEATFWVYYGTYTRDTSR
                     GIYVGKFDANTGRLTEPELAAETRNPSFLAVHPTRPLLYAVGELAEFEGQRTGAVLAY
                     QIDSQSGKLVAINRQPSGGTGPCHLVVDPSGRFVLVANYGGGSVASLPIRPDGGLEPP
                     ACVLRQEGRSVHPTRQTAPHAHQINFSPKGDLVLVPDLGLDKVLLFRWSPADGQLTEY
                     DPPFVTVPAGSGPRHFAYHPTGRALYVLNELTATVCVFECASGVPTRLVETVSSLPQD
                     FSGSNTAAEIVVHPTGRFLFTSNRGHDSVAVFRIADDALKISLMENVSVQGKTPRFIG
                     IDPTATFLFAANQDSHNVVVFRIDVESGHLTPTGQVINLGSPVCMIFRK"
     gene            complement(6870..8306)
                     /locus_tag="PKI05_00575"
     CDS             complement(6870..8306)
                     /locus_tag="PKI05_00575"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOA50717.1"
                     /translation="MTINLPVRLLPVTEYWDCHGCGICCRHVIIPLSEEEYERIRGQG
                     WDKHPELAGHRLFVRTRFGTSKYRLAHRPDGFCVFLSPQGRCRIHEKFGADAKPLVCR
                     MFPYQLVPLERFAYLTLRHNCPSVISQQGRSLAEQEEDWRPLVENQRFQMTLTPPPPL
                     TVTYRGNWNQFLYVANAVERFLTDTRFPMVRRLAHAVIFAQLLDRCRLARLNAQQFVD
                     LVGLLERGITKEVAPLFNQRVAPNRTAQLVFRRCLIEYYRLHPGSRLEAGWRGRFAWI
                     LTSWSMATGRGRIPSLLGPERCLRVAATIPLAGEVSPWADGGQTGGNDQLPVGSEMTD
                     LAASGVENDGESAETHDGKKAFADWIDLAVLDNNLGAIHRDVLEPLDDYFEKMAISKR
                     YAVCGRKGWSLTDRVRALATSFSVGLALLRLACPDGTPTRDDVAAVVIALDRGETYRT
                     LASSIFRRRLRILDRMDQLVRLLLWYAR"
     gene            complement(8334..>9395)
                     /locus_tag="PKI05_00580"
     CDS             complement(8334..>9395)
                     /locus_tag="PKI05_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOA50718.1"
                     /translation="PESEAAVEKGLAFLAKIQFADGHWSLNRLPRTLAGDDKTFGLGQ
                     MHGDTAATAMALLAFLGAGYTHQEPKYKDTVDRGLRWLIRNQLNNGQLFRSETDSTQY
                     TQIYGHGLGTIALCEAYGMTRDPALREPAMKAIDYIVRSQSPQFGGWRYVPRKETDTS
                     VTGWQLMALKSAQMAGLIVPSDAFQGVARWLDQAQVDDGARYVYNPYAGQTASQSHLR
                     LPNWAMTAEGLLMRIYLGWQRDTPALRRGVEYLKQNMPSYTSGSAVVRDCYYWYYATQ
                     VMFQMQGETWQLWNQRMQETLLKEQVQTGPWAGSWDPIQPVADRWGAEAGRLYVTAFH
                     LLILEVYYRHLPLFKSLAP"
BASE COUNT         2136 a         2723 c         2539 g         1997 t
ORIGIN      
        1 gggggcgaat tgacctcgac gtggcctccg ccaagttcgg cggtcttaat ctgtcgccgc
       61 aacagcttcg ccaggtggag aggattgtgc tcacgggttg cggaacgagt tggcacgccg
      121 gcttggtggg cgaatatcag atcgaagcaa ttgcccgaat tcccgtggaa gtcgaatatg
      181 ccagcgaact ccgttatcgc aatcctccgc tccatcgaaa cacgattctt tttgccatta
      241 cccaaagcgg tgagacagca gacactctgg ccgcccttcg agagttgaaa cggaaagggt
      301 atcccacact ggcgatatgc aacgtggtcg gcagcaccat cgctcaggag gccgatggcg
      361 gaatttatct gcacgcgggt cccgaaatcg gtgtggcgtc aacgaaggcc tttacatcgc
      421 aatgcctcgt cttggccttg ttggcgcttt attttggacg tttgagggac cttagctttg
      481 aggcgggcac ccgtatcatc gagaagctgc gggaacttcc caatcttgtg gcccgcgccc
      541 tggaatgtga cgaaaaggtg cgacgaattg ccgaccgctt ttaccagtgt cggaatttcc
      601 tgtatctagg gcgccagttc aattttccca cggccctgga gggtgcactg aagctcaagg
      661 agatcagcta cattcatgct gaggggtatc ccgcggcgga aatgaagcat gggccgattg
      721 ccttggtgga tgaacagacg cccagtgtct ttcttctccc ccaggggcat gtctatgaaa
      781 aggtgctggc caatatgcag gagactaagg cccgcggtgg accgatcatc gccgtcgtgg
      841 aagagggtga ccaagaggcc tggcgaatag ctgatgagat catcgaggtt cccgccgtcg
      901 atgattttct tcagcccatc gtctttatca tcccgcttca actcttggcc tatcacattg
      961 cggtggcacg agggtgcgac gtggacaagc cgcgcaatct tgccaagagc gtcacggtgg
     1021 aataggctcg tcgttcacat ttgggcagtt acgccggagc cttgggtaaa acccgcggat
     1081 agggcggctt ttctttcggc ggaagaggct gctgataggt ggtcgcccag gcctcccgtt
     1141 ccgcgtcggt ggcgtaatac cgcagccaca cctccggatc accttccaca aacgcgcaat
     1201 caaagcggag atagttgtta cccaaatcga cctttttctc tcgcgacggc aagatatcgc
     1261 ggtagatcag gcagtacagt tcgcggtccg agaggtgatc agtaaactcc agcacaatcc
     1321 gcttctgata gagcttgtga atcgtatccc aaaggatggc gtgcaattgc tcgtcgctga
     1381 gagtttcagg gcggggaagt cgtaattccg gatcaaacca ccgataaatg gggagaacgg
     1441 gggccatctc ccacgccagc atgctggcaa ggtattcatt ttccgccgcc aatggccact
     1501 gatggatgtt gacccgcgtc aaagaatcgt cgtaataagg ctccagctcg gtccgcaact
     1561 cgacattgcg aagcagaagg tcaatctcaa agttgtctcg atgttttttc ttggccattg
     1621 ccgatcgttc ctcaatctcc cgctgcaaat ccctgctgcc tagttggaaa cgtttgaaac
     1681 aagttgagac gacacgtgtc cccactatca ggaaccacaa ctacccgatg agacacgacc
     1741 acgcgacatc acgccgcatt catcggtccc catattcgac tgtactcacg catgaggcgg
     1801 cttcaagtcg tcagaagccc caaaaatctt caaagagaat cgcggcggtg gcaaaagccc
     1861 tacagccgaa aaatctgatg ttcctcgggc cgaacaatca gagaagacga caatatttcc
     1921 tcaagttttg caagcagccg tcttttcttg ttttgccacg atgcgataca cgatcaccca
     1981 tcagcctgcc gcggtctatt gggccttcgt atcattctct tccgaaaatg attgctgggg
     2041 atcaggacag caacacttct cttttaactc cttttcaatg gctgcgatct tctcgacacg
     2101 acgcgcgtgc cgcccacctt cgaaaggtgt attgagccag acctcgacaa tccgggtgat
     2161 cagccgttcg cccagcaaat ctgccgacaa gcagagaata ttggcatcgt tgtgacggcg
     2221 gctcatttca gccgtcaaat catcatggca aggaaccgca cgaattccct ggatcttgtt
     2281 ggccgcgatg cacatcccca agcccgttcc acaaaaaaga atgccgcggt ccacttcgcc
     2341 ggagctaacc ttccgtgcca cggcttccgc cacatcggga taatccacgg gagactgact
     2401 gaatgtgcca acatcgatga cctcgtgccc catattcttg aggaaggcca caatcttggg
     2461 acgaatactg taaccacgat gatcactgcc aatagcgatg cgcattgcaa aaacctctaa
     2521 acgttaagac tgtgatccaa atttgttgtt tgttttccta aattcccttg gctgtgcaac
     2581 caagccatta caattagtca cgtgtgcggt gccttgccac tgccaattaa ggccaacagt
     2641 tcgtcacatc gtcgcaacag ggcttcctct atcataccag cggacatccg ataggtttcg
     2701 accgtggccc caaagggatc gcaaatgtcc tttccctcgg gatgcaacag gaagacccga
     2761 tcggccgcct ccggccattg tgccacgatg gcgttccgga tagcctttgt catggcaaac
     2821 acgcaatcgg cctcccgaac gaaccgctcc gtcaaaggcc gtgacacgtg gttgctaata
     2881 tcaaatcccc gttcgtgcat gatctgaacg gcgtggggac tcggcacgtc accacagtag
     2941 gcctcaaggc cggccgaact gacaaccacg ccgcgccatt caaggtcctc gctcgaacaa
     3001 ccaagtttct ccgctaacat gtgccgacaa atggcctccg ccatgggact ccgacacgtg
     3061 ttacccgtgc agacgaacag gatcacggcc gcggccatgc gtcgcacgta ttcctcactc
     3121 aagcttccct gacgcaagat ttcccagccg tcctcggtca cccgaaccac ggtcgatgcc
     3181 acaccgagct gggaaggtcc gtcatcaaac acaatatcca aacccggcag tcgcttgact
     3241 acctcctcag ccgtcgtcgc aggggcctca cccgatcggt taacgctcgt caagacaagc
     3301 ggcgctggca acaaccgcaa cacctcagcc acaaaagggt gctttggcac gcggaaaccg
     3361 atactccctt ccggagatac caattcccga acacacgggg caaacctctc cacgacgctc
     3421 cttcccccct cagccttgag gactagggtc atcggccccg gccacgcccg ctgcgccaac
     3481 ctccagccaa cgcggttcag gttgggaacg tagtcccaga gttcctcgcc acttttaatc
     3541 gccagggtga acggatgacc ggccttgcgg cccttcaggg cggcgagacg ctccacggcc
     3601 tccgggacta acacggatgc cgctacacca tagatggtct ccgtcggcaa ggccacgagg
     3661 cgtccttccg ccaaagcctg cacacagcga tgaacgagat cgcgcggttc ctcggcctgc
     3721 ctcaggggga tgactaacgg tgaatccatg atcgggctga cgagcagtct cctaagcggg
     3781 ttcgcatggg attgtgtcgt tgttcatact ctgccttccc cttcgaccag ttataggagt
     3841 gaccaccaac tttggtcaac ccatttggcc tacagcaccc catgccaaac cttatccaga
     3901 cgaagaagtc tccgccaagt aacctgaaag agacgccgca acggccgggt cagtttcctt
     3961 ggccaggtgt gcgcgaatga ttgggccagc ggcggcgata tcgattcgcg ccaaagccca
     4021 ggcacaggcc tcccggacaa cgggctcgga atcttcaagt ccgctcacca agacgtccca
     4081 ggctgcctga ttacgttgat tccctaacac gatggccgcg ttgcgcaaca ggcctcgcct
     4141 ttttgccctc cacaaggccg ttgggccaaa catcttgcga aactcatcct ccttcaaccg
     4201 aaaaagcgca atcaaattga tgacgcgggt ctcggtagcc tccgaagttg cttgggccgt
     4261 ggggcccagt ctccgattcc aggggcacac ctcctggcac aggtcgcagc caaaaaacca
     4321 gttacccaca agctctcgca gttcccgagg aataaaacca cgtaactcaa tcgtcaggta
     4381 actgagacaa cgtcgggcat cgaggacgcg tgggcttacc aaggccccgg tgggacacgc
     4441 gcgcaagcaa gccgagcaac tgccgcaccg gtcaccgcca aacggcgggt cgaagtcgag
     4501 ttctgctgtg gtcaacagcg cggccaaaaa ggtgaagcta ccgcaatcgg ggccaatgag
     4561 caacgtgttc ttgccgatcc agcccaagcc agccaattgt gcaaagtcgc gttccaacaa
     4621 aggggctgta tcgacaaccc cgcggaccgc cgctgaggga tattctgcct gaaggaatcg
     4681 tcccagcttt cgaagccact gccgaatggt cttgtggtaa tcggggccat tggcgtagcg
     4741 tgcgatgagc ccataaccag caggtgggtt ctgctctgca tctctttgat acgggaagcc
     4801 cagcatgatc aggcttcgtg cgccgggtag gatggacgac gggcttcgat acgcctcctt
     4861 tctctgggcc aaatagtgca tctccccgtg gtaacccgcc tcaagccact tgagaaaggc
     4921 cccataatgg ggcggctcca ccgccgggca aatgccaaca caacagaagc ccaggtcttt
     4981 tgcaaggtct ttcaaacgaa ttacaatgtt gagtggcatg cggaaataac gttacgtttg
     5041 gacggctcgc ttgaaccggg tcagtttaga gcctatccca aaaagtcccc ctctccctcc
     5101 gggagagggg agattacgag ataggctctt aattcgcgat gacccaatcg atcgttgctg
     5161 tcgcggtttc gtttccgccg tcaaaacggt gtggtcatcg gatgcactgc gacgcgctat
     5221 agctatactt gggaatactt tgggatcatt taagtccata gggattatac aagaacgatg
     5281 atcatgagac acacaaagct gcggattaac ccgacaattt gggcactgag tgcgatcggt
     5341 ttgctcggcc tattctcttt cgcagggtgc cagtcacggc ctcccctaga agagttgggc
     5401 gaactcgaat ttgccgtgcc ggatatcccg ggaatcgatg acccgtaccc gttcccggat
     5461 gtcgcaggca gtgccgagaa accggcagat tcggaacagt cggacgatga accgtgatca
     5521 accggccaac aagcgttcgc agttgttcga gtggtctata caagtaaagc gcgttggccg
     5581 tgggtgagtc gcgaaatact tcggtaatct atctctgcgg atgaaacgca acgtgggacc
     5641 caagagtagc caataggagg ccatcaaatg ttgtggcgat accttatgtc ctgtgcatgt
     5701 ggcgtggttg tttttctagc aatggatggg caaaagctaa tgggcgaaga ggccaccttt
     5761 tgggtctatt atgggacata cacgcgagac acaagtcgcg gtatctatgt tggaaaattc
     5821 gatgccaaca cggggcgact gacggaaccg gaactagccg cggaaacgcg gaatccttct
     5881 ttcttggcgg ttcaccccac gcggcccctg ctttatgccg tgggtgagtt ggcggagttt
     5941 gagggtcagc gaaccggggc ggttttggca tatcaaattg atagtcaatc cgggaaactc
     6001 gtcgccatta accggcaacc ttctggcgga acggggccat gccatctcgt tgtggatcct
     6061 tcgggacgat tcgtgttggt cgccaattac gggggaggta gtgttgccag cctaccgatc
     6121 cgtccagacg ggggactcga accgcccgct tgtgttctac gtcaggaagg gcggagcgtt
     6181 cacccgacca ggcagacggc cccccatgcc caccagatca actttagccc caaaggggat
     6241 cttgtcctcg tgcccgatct tggactggac aaagtacttc tgtttcgatg gtcgccggcc
     6301 gatgggcaac tcacggaata tgatccaccc ttcgtgaccg tcccggcagg ctccggtccg
     6361 cggcatttcg cctatcatcc cacagggcgg gccctgtatg tccttaacga actgacggcc
     6421 acggtctgcg tgtttgaatg tgcatccggt gtgccaaccc gattggttga gacagtgtcg
     6481 tcgttgccgc aggatttctc aggctcgaac actgccgcag agatcgtggt acacccgacg
     6541 ggtcgctttc tctttacctc gaaccgaggg cacgactccg tcgcggtgtt ccgaatcgcc
     6601 gacgatgcct tgaaaatcag cctaatggaa aatgtcagcg tgcaggggaa aaccccacgc
     6661 ttcattggca ttgatcctac ggcaacgttt cttttcgcag caaaccagga ttcccacaac
     6721 gttgtggtct tccgtattga tgtggaatcc ggacatctga caccgacggg gcaggtaatc
     6781 aatcttggct caccggtgtg tatgatcttc cggaagtgac agcccttttg tcgcgccaat
     6841 tggccaaaga ggcttcacct gagatcaatt taacgcgcgt accacaagag aagtctgaca
     6901 agctgatcca tcctatcgag gatgcggagt cggcggcgaa agatcgaact ggccagtgtg
     6961 cggtaggtct cgccacgatc gagggcgatg accacggcgg ccacgtcatc gcgggtggga
     7021 gtcccgtcgg gacaagccag ccttaaaagg gccaaaccaa ccgaaaagga tgtggccaag
     7081 gctcgcaccc ggtcggttag cgaccagccc tttcgaccgc aaacggcata tcgttttgaa
     7141 atggccatct tttcgaaata atcatcgagg ggctccaaga catctcggtg gatggcccct
     7201 aaattgttgt ccaacaccgc cagatcaatc cagtccgcga aggccttctt gccgtcatgg
     7261 gtttcggcag attccccatc gttttcaaca ccgcttgcag ccagatctgt catttctgaa
     7321 cccacaggga gctgatcatt tcctcccgtt tgtccaccgt cagcccacgg agatacttca
     7381 cctgcaagtg gtatcgtggc ggcaaccctg aggcatcgtt caggtccaag aagggaagga
     7441 atccgcccgc ggccagtcgc catgctccat gacgttagga tccaagcgaa gcgtcctcgc
     7501 cagccggctt cgaggcgaga accagggtgg aggcgatagt actcaatcaa acacctgcgg
     7561 aacacgagct gcgccgtccg attgggggct acgcgttggt taaataacgg agcaacctct
     7621 ttcgtgatcc cgcgttccag caggccaaca aggtcaacaa actgttgggc atttaagcga
     7681 gccaaccggc aacgatcgag aagctgcgcg aatatcacgg cgtgagccag acgtcgtacc
     7741 atcggaaaac gggtatccgt cagaaaccgt tcgacggcat ttgcaacata caagaattga
     7801 ttccaatttc cccgataagt cacggtgagt ggcggcggtg gagttaaggt catttgaaaa
     7861 cgctggtttt ctaccagtgg ccgccaatcc tcctcttgtt cggcaagcga tcggccctgt
     7921 tgcgaaatga cactgggaca attgtgacgc agcgttaggt aggcaaatcg ctcgagtggg
     7981 acaagctgat agggaaacat tcggcaaacc aagggcttgg cgtcggcacc aaatttctcg
     8041 tgaatacgac accgcccctg agggcttaga aagacgcaaa acccgtcggg acgatgagcc
     8101 agtcgatact tggatgttcc aaaacgggtt ctcacaaaca gacgatgccc agccagttcg
     8161 ggatgcttgt cccaaccttg gccacggatt ctttcgtatt cctcctccga caaagggatg
     8221 atgacgtggc gacagcaaat accgcagccg tggcagtccc aatattccgt aacgggtaat
     8281 agccggacgg gaaggttgat ggtcatgggt taagatatgt tcttccagtc gctttaaggc
     8341 gcgagcgatt tgaacaaggg caaatgtcga taataaacct cgaggatgag gagatggaat
     8401 gccgtcacgt agaggcgacc ggcctctgct ccccagcggt cggccaccgg ttggatcggg
     8461 tcccaactac cggcccacgg ccccgtttgg acctgctcct taagcaatgt ttcctgcatc
     8521 cgctggttcc acagttgcca ggtctcgccc tgcatttgga acatgacctg ggtagcgtaa
     8581 taccagtagt aacaatcccg caccacggcc gagccgcttg tgtaactggg catgttctgc
     8641 ttaaggtact caacgccgcg acgcaaagcc ggtgtatccc gttgccaacc gagataaatt
     8701 cgcataagaa gtccctcggc cgtcatggcc caattgggca accgcaaatg gctctgcgac
     8761 gccgtctgtc cggcgtacgg attgtaaaca tagcgtgccc catcatcgac ctgtgcttga
     8821 tcaagccagc gggcaacgcc ttgaaatgcg tcgctcggaa caatcagacc tgccatttga
     8881 gcgctcttca gcgccatcaa ttgccaaccg gtcacagagg tatccgtctc cttgcgcgga
     8941 acatagcgcc agccaccgaa ttgcgggctt tgcgatctca caatatagtc aatcgccttc
     9001 atggcgggtt cacgcaatgc cggatcgcgc gtcatgccat aggcctcaca aagggcgatt
     9061 gtccccagcc catggccata gatctgggta tattgcgttg aatccgtttc agagcgaaat
     9121 aattgtccgt tgttcaattg gttcctgatc aaccagcgta agccgcgatc aactgtatcc
     9181 ttgtatttgg gttcctgatg ggtgtaaccc gccccaagaa aggcgagcag tgccatggcg
     9241 gtggctgccg tatcgccgtg catttgaccg aggccaaagg tcttatcgtc gcccgccagc
     9301 gttcgtggca agcggtttaa actccaatgt ccgtcggcga actggatctt tgcgaggaag
     9361 gccaggccct tttccacggc ggcttcgctt tccgg
//
LOCUS       DAOPTR010000014         8904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_6528_length_8904_cov_3.358911, whole genome shotgun sequence.
ACCESSION   DAOPTR010000014 DAOPTR010000000
VERSION     DAOPTR010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 8904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8904
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6528_length_8904_cov_3.358911"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="PKI05_00585"
     CDS             complement(<1..862)
                     /locus_tag="PKI05_00585"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOA50719.1"
                     /translation="MTFWLAYLSNALVSAGNVLLCRYADYVNFLGADEYHLGWIIGFG
                     MVGSLFMRFALGSGIDRFGARRVWLLTILATALGCLIHLGITDYRGIAIYLARVFYII
                     SLAGVFSASMTLMAARAPRERLAEVVGIYGSAGFAGVLLGAPLADLLFPAGITSMGEA
                     RLLFLTAGGMCLLSFPLAWFVPPERTGRPRVNQARTESLKTVRRSSVKEPGLFRILLR
                     YTSVPILIAGIVAGAGLSVPPTFLPRYVAEFGVHSVSGFFFLYAATAIVIRGILLRRV
                     SNPRLDRMILL"
     gene            complement(1889..2338)
                     /locus_tag="PKI05_00590"
     CDS             complement(1889..2338)
                     /locus_tag="PKI05_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOA50720.1"
                     /translation="MTRTLVPWTTSPSRLFESLRREMNELMDQFFGSDGGTDLTVWFA
                     PRMNLAETDQAFEVTVDLPGMKPEDFSVELKEGQLWITGERRQETEEKGKTYHRVERQ
                     YGRFQRVIPLPAAVNPEKIEAQYKDGVLHITLPKDETAQPKRIPVKT"
     gene            complement(2736..4745)
                     /locus_tag="PKI05_00595"
     CDS             complement(2736..4745)
                     /locus_tag="PKI05_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50721.1"
                     /translation="MRAFSWAFSLLVLLEFGCLVTDGHSGLTAQEVPPTTPLLSTTAS
                     QPASPEASRLQVSIGQAENSPAGAVSPKDTALRIESPNNPVSESAALPSESPPTSVAP
                     RPDPKTNTAPLTVEAAAFNGVTPGVSHVNDLTRQWGPPKEAVTKGDSVYYLYEIPPFD
                     RIEVHIQNDKVEAIIIRLDKTYPANLVAEHLELSQFRPVLVSNPQGEVLGQTFPERGV
                     LFSFAPAPEPGKSSNLVTEIILSEVTAEPFILRAETFWQTEPDSAIRDLEQALKFDNR
                     AHRAWWLLARIQKELGSLKEALASAQNAVNLDPASLHYRLTLAEILDEVGQDNLAIQQ
                     LEQVVAASDQRPHIKARALCNLGNIYQSLPQPDYKKALDYHYEAVKIAQPLRSDVHPA
                     IRVAAKEVLVDAHLGAALDIAWGPWEQKEAVVPRWLERAANLAQDLVNTEQQSADKLF
                     RVSTTALATSVAMPRVIGPLPWIEALDQAYQRLEAEASEVRRARLRWNYGVALYDAVQ
                     CFQVLGEQELAQQYGKKAAEALETGLQGRSPKGSDLYLLGRLYFRMGAVDAIQNKDHK
                     QAVTYFDKAIPLLRQAAGDIPETSIGRLGETFVSMGVSYWETGDRDQAIALTEEGLQW
                     MEKAVQLGLLANSALDVPRANLQTMRQARQRLAERTSSDTAATQR"
     gene            complement(4955..6415)
                     /locus_tag="PKI05_00600"
     CDS             complement(4955..6415)
                     /locus_tag="PKI05_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA50722.1"
                     /translation="MFGKVSRRRVLAGLTSVVAVPTLVRSSALGLAGLAPASERVTLG
                     LIGCGGHGIGWNLAMVFRHNDAQVIAVCDVDQRHLAAGKQRVDEHYRNVVGEQYRECA
                     AYRDFRDLINRRDIVAIMNVTPDHWHVLPALMASECGKDVLCEKPLTLFVEEGKVLCQ
                     TVAKNKTVFQTASENRSIDVYIQLVSLVRAGVIGKLKHIEVRLPMGNTNTRVVGEAKD
                     MFYQSSPQEVPAYLDYDMWLGQAPWMPYIPARLHGNFRWNLAFSGGVLTDWGAHMIDL
                     AQWGHNTERTGPVEVEGRGDFPPRDAVFNTAPTFDLLYRYSDGVTMRVSAGQGDLDPQ
                     QVKPDTPLVGRTPSPGIRFEGTDGWIESHNWRGVLRASRSEMLKVEVDPKALGIYIPG
                     EVVARDSGRGGEHRNFLDCIKSRQPCYAPAEVGHRTITIAHIGNIAMLLGRKLRWNPD
                     KEEFLGDDEANAMLTRKQREPWTMANVTSWIKSGNS"
     gene            complement(6574..>8904)
                     /locus_tag="PKI05_00605"
     CDS             complement(6574..>8904)
                     /locus_tag="PKI05_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50723.1"
                     /translation="LEAREKALKEDREALVRDRESLAEERQTIERLRKEQQARAEELL
                     TLQTSLAQREQEVREKQQLLAEREASFKQKAEQLFEEARRLAALEQELKCRTDALNAK
                     EQDLSRRESEQREAAIRFADRELRLSQQQEMLLRLEQELESRKDELTRLEQGLLRQQK
                     ELSIRQQALSEQEQLLSSRCQELANLASHLAEKEREIATQASNLARWEQNLQEWERDV
                     KDRASRLPVVAPTEGAAESEIATQTTGNEQELATPQRVESEVVEAQPAEGISGAEGFS
                     SVTAESSVAELPGAVLATSIAPRVEIDRLREEDWPVIPLAKIHRRWRAPAPIAIGLVI
                     AGLVACSVWWFCPREFITACRIEFPEDSPLLYVRDGTRPRGVVEEFFPSMPAVAANDP
                     RVVTRLGQIPEIPTLLTNGEKTWDQVLASAKVSRSEGLPFTTMVITTRDPTKTQQAIE
                     RWGNVYLAMLAESFQEKVSPALAKAKQRWQITSQQLKEHQASINDLKQRYQVEHPRQL
                     ELQAHESLHMAEVTQKEIDQLQAEITAQKQRLEQVKAVADRPEQAISEEEVMAAVAER
                     LAQDGLTVETSDPGADAVNPDPGIADEAEANSQQPPDGSNAPRLNAPDPGSRYEIVRQ
                     LVRKELSERKRQDAVLTVQVLTAELDRLQARLQKAMEQKAQYEATAGALLEAAKQWNN
                     LEVETAKLENEHQQLTTQIDILEKALAQSRIAPHLEIWSSQQRPWMPFAAAALAGGGA
                     MIPFGLWGLLRSLRTVRVFPPAAIGYQSKYPHPTPD"
BASE COUNT         1891 a         2544 c         2454 g         2015 t
ORIGIN      
        1 caaggaggat catacgatcc aatcttggat tggagacccg acggaggaga atgccccgta
       61 tcacgatggc ggttgcagcg taaagaaaga aaaacccgct caccgagtgg acgccaaatt
      121 cagcgacgta ccgcggtaag aatgttggag gaaccgaaag cccggcacca gcaacaattc
      181 ctgcaatcag gatgggcacc gacgtatagc ggagcaaaat gcggaaaagc cctggttcct
      241 tcacagagga tcgacgaaca gttttgagcg attccgtccg agcctggttg actcgcggtc
      301 gcccagtcct ttctgggggg acaaaccagg ccaggggaaa actcagcagg cacatgcccc
      361 ctgccgtcag gaacaagagc cgtgcctcgc ccatcgaggt gattcccgca ggaaagagca
      421 agtcggccag tggggcacct aagaggactc ccgcaaaccc cgcggaaccg tagatgccga
      481 cgacttctgc gagtcgctca cggggagcgc gggcggccat gagagtcata gaagcactaa
      541 agacaccagc cagggaaatg atgtaaaaaa cacgggccaa atagatggca ataccacgat
      601 agtcggtaat tcccagatgg atgaggcatc ccagggcggt ggcgaggatc gtgaggagcc
      661 agacgcgacg tgccccaaag cggtcaattc cactgcctaa ggcaaaccgc atgaataggc
      721 tgcccaccat tccaaagcca ataatccagc caaggtggta ctcatcagcc ccaaggaagt
      781 tcacgtaatc ggcatatcgg cagagcagga cgttgccggc cgacaccaaa gcattcgaga
      841 gatacgccaa ccagaacgtc acgtcgtaga tgcatcgtgg cgagactgat gatgggagtg
      901 aaccggcagc gggcaggaca gaggaagcaa tccttcgacc gttgtcggat gtcggggggt
      961 tttgttggcg tcggggatga atgctcacac ggagcagaga gagataatca cgactcatac
     1021 gtgtttgggg caagccgcac agttggctat gaatggaaat caagcccact caaacgggcg
     1081 agcgggcctt gctgcatgta cgtcactgaa tcgaatcggc agatccaccg ttccggaaat
     1141 aggagagatg acggcccact tcggttcggt ggagaagcgt cgccgcgtgt atttcgtcat
     1201 atcatacgct gagagtcgaa aaccgccaag ctcgttattt cattttcttg gggcgatgca
     1261 ccttcgatcg actactgctg tatcgcacct tggccagtct ttacctggtg agtctttgca
     1321 cagatcgttc cgcggttcat ctgacaagat gaacaccctc tctgtactac aggcagaact
     1381 cccaccggac ttcgccgcgc cgagtttcgc ggcatgttgt ggcgatccag ctggaaacag
     1441 gtgcgaaacg aggttctctc acctgacccg gcaactgcac cgggaagaaa tggcctcgtc
     1501 gaacgagata ggcctgccta tcctgccccg tttctcgacc catcacttaa atattgccca
     1561 cttgaaggga aacttgacga caggtgactt caatacagca gctaaagttc ggaaaaggca
     1621 acctgagatc gcttttccac aaattgccaa aaaaggcgtc ctgtgtggaa aataccatcc
     1681 cgcaaaggtt caaaaaagaa aaaggccccc ggggtgggcc atattcctgt ttacacggga
     1741 ccgtcaagga ggattgccaa gtcgctgcgg cgagctacag ggcgcctggg ctgcatagga
     1801 ggctgccctt tacaagggtc catccttgcc caccccgtcg gggatgatag gcactccttg
     1861 ccctttaacc caacccagcg gacccagatc aggtcttgac tgggatccgt ttcggttggg
     1921 cggtctcgtc cttgggcagg gtgatatgca gaacaccgtc cttgtactgg gcctcgatct
     1981 tctctggatt gacggctgcc ggcagtggga ttacccgttg gaaccggcca tattgccgtt
     2041 ccacacggtg ataggtcttg cccttttctt cggtttcctg acggcgttcg ccggtaatcc
     2101 agagttggcc ctccttcagc tcgaccgaga aatcctccgg tttcatcccg ggcagatcaa
     2161 ccgtcacttc aaacgcctga tctgtctcgg cgaggttcat ccgcggagca aaccagacgg
     2221 tgagatcggt tcccccgtcg ctgccgaaga attgatccat caattcattc atttcgcgac
     2281 ggagtgattc gaacaaccgg cttgggctag tggtccaggg taccagggtc cttgtcatgg
     2341 ccacacctcc tgctatcgct taccgtcgcc accgagcatc cccctttgcc tcgattcagc
     2401 cggtggcatc ccaaatcgag gctaccaccc gaccagattt cgtagagagc tccgtagtga
     2461 agaggacttc gttgatcagc cgtcggattt aaatgcaatc cgcgtgccaa aagtccccag
     2521 agcaactttt tcccgcggtt acaatgcctt cacaagctcg taagttgttt tccaataata
     2581 acttaggtag acacagcgct tgccagttac tgtgggaaga tgcttgcgtc gagggagtga
     2641 aagaactgcc aaattggcat gaactcgaca gcctgttcac ttgggaaagt gctgatttta
     2701 ttttcagagg gctgcttgtg cgaagggggc tgagtctatc gctgcgtagc cgcggtatcc
     2761 gaagatgtcc tctcggccag tctctggcga gcttgacgca ttgtctgtaa attcgccctc
     2821 ggcacgtcta aggcagaatt ggcgagtagg ccgagttgaa ccgccttttc catccactgg
     2881 aggccttcct cggtcagtgc gatcgcttga tcccggtcgc cggtttccca ataggagacc
     2941 cccatgctga caaaagtctc tcccagccgt ccgattgagg tctcaggtat atcgcccgcg
     3001 gcttgacgga ggagaggaat ggccttgtcg aaataagtga cggcttgttt gtgatccttg
     3061 ttttggatcg catccacagc gcccatcctg aaatacagtc ggccaagcaa gtacaagtcg
     3121 ctacctttgg gtgagcgtcc ttggaggccg gtttccaggg cctcggcggc cttttttccg
     3181 tattgttggg ccagttcctg ttcaccgagt acctggaagc actggaccgc gtcgtatagg
     3241 gcaaccccgt aattccaccg cagacgagcc cttcggactt cactcgcttc cgcttcaagc
     3301 cgttggtagg cttggtccaa tgcttcgatc caagggaggg gaccaattac ccgcggcatg
     3361 gccacgctgg tagcgagggc cgttgtcgag acgcgaaata gcttatctgc ggattgctgc
     3421 tcggtgttga ccaaatcctg agctaaatta gcggcgcgct ccagccatcg tgggacaact
     3481 gcctcctttt gctcccaggg tccccatgca atgtccaggg ctgccccgag gtgggcgtcg
     3541 acgaggacct ctttagccgc cacgcgaatg gccggatgta catcactgcg gaggggttgg
     3601 gcgatcttga cggcctcata atggtaatcg agcgccttct tgtagtcggg ttgcgggagc
     3661 gactggtaga tgtttcccag gttgcataat gcccgggcct tgatgtgtgg acgttgatct
     3721 gacgcagcca ccacctgctc gagttgctga atggcaagat tatcttgtcc cacctcatcg
     3781 agaatctccg ccaaggtcag gcggtagtgc aaacttgccg ggtcaagatt gacggcattc
     3841 tgggcactcg ccagggcctc tttgagactt cccagttcct tttgaatgcg ggccagcagc
     3901 caccaggcgc gatgggctct gttgtcgaat ttcaatgctt gttccaggtc acggatagcg
     3961 ctgtcaggct ccgtttgcca aaaggtctct gcccgcagaa tgaacggttc tgctgtcacc
     4021 tcggacagga tgatctcggt cacgagattg gatgattttc cgggttctgg tgcgggagca
     4081 aaactgaaga ggacaccacg ttcgggaaac gtttggccaa ggacctcgcc ctggggattc
     4141 gagacgagca ccggtcgaaa ttgagacaat tccagatgct ccgccacaag attagcggga
     4201 taagtcttgt ccagccggat gataatcgct tcgactttgt cgttctggat gtggacttca
     4261 attcggtcaa aggggggaat ctcataaagg taatacaccg aatccccttt tgtcacggct
     4321 tccttgggtg ggccccattg gcgcgtcagg tcattcacat gggaaacacc cggggtcacc
     4381 ccattgaagg cggctgcctc cactgttagc ggcgccgtat tggtcttcgg atcaggccga
     4441 ggcgcgaccg aagttggggg ggattctgag gggagggctg ccgactcact caccggatta
     4501 tttggcgatt caatccgaag ggccgtgtcc tttggtgaga cggcgccggc gggactgttc
     4561 tctgcctggc cgatggagac ttgaagacga ctagcctcag gcgacgccgg ttgcgacgct
     4621 gtcgtcgata acaaaggcgt tgttggagga acctcctgcg cggttaatcc ggagtggcca
     4681 tccgtgacca gacatccaaa ctccaacaaa acgagtagcg agaaggccca agagaaagct
     4741 cgcatgccaa attacctcca tgaattcgtg gccacagtca ataaagccac gtgcggttcc
     4801 tctccgcaga cttacggcgc cggtaatgta cctcagaccg gttcaccccg tccagagcaa
     4861 ttttgccatg tagagggaca aaataccccc gcccaagtgt gggatagttg cgtagagtgg
     4921 acgggggcag gcttcccgga tctaaagcca cgccctagga gttacctgac ttgatccagg
     4981 atgtcacatt ggccatcgtc caaggttctc gctgtttgcg ggtgagcatc gcgttggcct
     5041 cgtcgtcacc caaaaattct tccttatcgg gattccagcg aagctttcgc cctagcaaca
     5101 tggcgatgtt gccgatgtgg gctatggtga tggtgcgatg gccaacctcg gccggggcgt
     5161 aacacggctg ccgcgacttg atgcaatcga gaaagttgcg gtgctcgcca ccccgtccgg
     5221 agtctcgggc gacaacctct cccggaatgt agattccgag ggccttggga tcgacttcca
     5281 ccttgagcat ctcgctgcgg ctcgcccgaa gcactccgcg ccagttatga gattcaatcc
     5341 atccgtcggt cccttcaaag cggatccccg gagaaggagt ccgcccaaca aggggcgtgt
     5401 cgggtttcac ctgctgggga tcgaggtcgc cttgtcccgc ggacacccgc atcgtcacgc
     5461 cgtcactgta tcgatagagt aaatcgaacg taggcgcggt attgaagact gcgtcccggg
     5521 gcggaaagtc gccgcggcct tctacctcca ccggccccgt tcgctcagtg ttatggcccc
     5581 attgggccaa gtcgatcatg tgggcacccc agtcggtgag gacaccccct gagaacgcga
     5641 gattccagcg gaaattgcca tgaagtcgtg ccgggatgta aggcatccag ggggcctgtc
     5701 ccagccacat atcgtagtcc aaatacgcgg gaacttcctg cggggagctc tgataaaaca
     5761 tatccttggc ctctcccacc acccgcgtgt tcgtatttcc catcggcagg cggacttcaa
     5821 tgtgtttgag cttaccgatc acacccgccc ggactaagct aacaagctgg atatagacgt
     5881 caatcgagcg attttcggaa gctgtctgaa agaccgtctt attcttggcc acggtttggc
     5941 agaggacttt tccctcttcg acaaagagcg tcaagggctt ctcgcagagg acgtctttgc
     6001 cgcattcaga ggccatcaac gccgggagga catgccaatg gtccggtgtg acgttcatga
     6061 tggccacaat gtcccgccga ttgatcaggt cacgaaaatc tcgataggcg gcgcattctc
     6121 gatattgctc cccaacgacg ttacggtagt gctcatcgac gcgttgtttt ccagcggcga
     6181 ggtgtcgttg atccacatca cacacggcaa tgacttgggc atcattatgg cgaaagacca
     6241 tcgcaagatt ccagccgatg ccatgtccac cacagccaat caaacccaac gtgacccgtt
     6301 cgctggcggg tgccaagccg gctaacccaa gtgcggacga acgaaccaac gttggaaccg
     6361 caaccactga agtcaaccct gccaaaacac gccgccgtga gacctttccg aacatatttt
     6421 gcgatctcct gcgagaaaaa cgggtgatat agtttctggc tcacacacca aggtgcgata
     6481 gtgttaacta cttggttcac actgcgttct gccgaacctc ccaagcgaac ttcctcccga
     6541 tggggcactc aacctggtcc tttctccgat tgtctaatcc ggtgtcgggt gtggatactt
     6601 agactggtac ccaattgccg cggggggaaa taccctcacg gtcctgagcg atcggagcaa
     6661 cccccacagg ccaaagggga tcatcgctcc gcctccggca agcgccgccg ccgcaaaagg
     6721 catccagggc cgttgctggg acgaccaaat ctccaagtgc ggcgctattc tactctgagc
     6781 gagtgccttt tccaagatat caatttgggt tgtcagttgt tggtgctcgt tctctaattt
     6841 tgcggtttcc acttcaaggt tgttccactg ctttgccgcc tctaacaaag cgccggctgt
     6901 cgcctcgtat tgggcttttt gctccatggc cttttgcaat cgggcctgca aacggtccaa
     6961 ctcggcggta agaacctgga cagttagaac cgcatcctgg cgcttgcgct ccgataactc
     7021 ttttcgcaca agttgtcgta cgatttcgta acggctgcca gggtcggggg cattcaatct
     7081 cggtgcattt gacccatcgg gaggctgttg actgtttgcc tcagcttcat ccgctatacc
     7141 gggatctggg ttcacggcgt ccgcaccggg atctgaagtt tcgaccgtca gcccgtcttg
     7201 cgccaggcgt tctgccacgg cggccatgac ttcctcttca gaaatcgctt gttcaggccg
     7261 atcagcaact gccttgactt gctctaggcg ttgcttctgt gccgtgattt cagcctggag
     7321 ctggtcaatc tctttttgag tcacttctgc catgtgcaac gattcatgag cttgcagttc
     7381 cagttgccga ggatgttcga cctgatatcg ctgcttcaaa tcattgatac tcgcctggtg
     7441 ctcttttaat tgctgagacg tgatttgcca tcgctgtttg gccttggcga gagcgggcga
     7501 aaccttttcc tggaacgact cagccaacat ggcgagatag acgttccccc atcgttctat
     7561 cgcttgctga gttttggttg gatcccttgt cgtgatgacc atggttgtga agggaaggcc
     7621 ctcggatcgg ctgactttcg ccgacgccag gacctgatcc caggtctttt cgccatttgt
     7681 taacaaagtg ggaatctcgg gaatctgccc aagtcttgtc acaacccggg gatcgttggc
     7741 ggcaactgca ggcatcgaag ggaaaaactc ctccactacc ccccgaggac gcgttccatc
     7801 tcgcacataa agcaagggcg agtcttcggg gaactcgatg cggcaagccg tgataaattc
     7861 ccgaggacaa aaccaccaca cagaacaggc gaccagcccg gcgatgacga gtcctatcgc
     7921 aattggcgca ggtgcccgcc aacgacgatg aatcttggcc agcgggataa caggccaatc
     7981 ttcctcgcgg agcctgtcga tctcgacccg tggagctatc gaagtggcta acacagcccc
     8041 cggtagttcg gccacgctcg actcagcagt gaccgagcta aacccttctg ccccagatat
     8101 gccttccgcc ggttgcgcct caacgacctc tgactcaacc cgctgcggtg tggccaattc
     8161 ttgttcgttg cctgttgttt gagtagcaat ttcggactcc gcggcacctt ctgtcggtgc
     8221 cacgacaggc agacgcgacg cccgatcttt aacgtctcgt tcccactctt gaagattctg
     8281 ctcccatctc gcgaggttgc tcgcttgggt ggcgatctca cgctcttttt ccgcaaggtg
     8341 gctggcgaga tttgccaact cttggcatcg agaagaaagg agttgttcct gttcactgag
     8401 ggcctgctgc cttatcgaga gttctttctg ttgccgaagg agcccttgtt ccagacgcgt
     8461 cagctcgtcc ttgcgactct caagttcctg ctcaagacga agcaacatct cttgctgctg
     8521 gcttagacgc agctcacgat cggcgaaacg aattgctgcc tcacgctgct cgctctctcg
     8581 acgagagagg tcttgctcct tggcgtttaa ggcatccgtg cgacatttca attcctgctc
     8641 cagggctgcc aatcttcgag cctcttcaaa cagttgttcg gctttttgct tgaaagatgc
     8701 ctctcgttcc gcaagtaact gctgtttttc tcgaacttct tgctcgcgct gagccagcga
     8761 ggtctgcaag gtcaacagtt cttctgcacg ggcctgctgt tctttccgca atcgttcaat
     8821 agtctgcctt tcttcggcaa gactctccct atcgcggacc agcgcttcgc ggtcttcctt
     8881 aagtgctttc tctctggctt cgag
//
LOCUS       DAOPTR010000015         8816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_6615_length_8816_cov_3.634402, whole genome shotgun sequence.
ACCESSION   DAOPTR010000015 DAOPTR010000000
VERSION     DAOPTR010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8816
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6615_length_8816_cov_3.634402"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..342
                     /locus_tag="PKI05_00610"
     tRNA            270..342
                     /locus_tag="PKI05_00610"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:303..305,aa:Gly,seq:gcc)
     gene            388..1920
                     /gene="tig"
                     /locus_tag="PKI05_00615"
     CDS             388..1920
                     /gene="tig"
                     /locus_tag="PKI05_00615"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOA50724.1"
                     /translation="MVASDGFRQDDEELKQASGLSEVEDADLMAPAATDESAGEAEES
                     TETPLEFEFDVEAAGPCKRKIIVTIPRKEVDRYFQEEFDKIRDEAQVPGFRPGRAPRK
                     LLEKRYRNELREPVKARLMLDAFTALADRANLAPISDPELDPDRIELPDEGDFAFEFS
                     VEVRPEFEIPNWKGLKLTKTVYSYTPQYFEAYLREQLKSEALLEEVEEPAQLGDYITC
                     DISLFHEGRVLNSSDGERLLIRPRLTFMDGVIEDFGERMVGVQRGESRTCDVRVAESC
                     SDPELAGKTIQARFKVHAVLRRRSAENLEAFAQTMGYGSVEELRDAYERRLRSRLDQM
                     TYESLRKQILDQLLDVVPFDLPQDLVKRQTERELRRRAVELVRAGYSQEDIQPHLNAI
                     AREARTQVVRLLREHFLLERLAEAENIEADEDAIDAEVERIAMQTGRSPRQVRAELDQ
                     EEAWDVIRNAIVERRMIEKIIEVAEITEEQKTLTAREEAEETALDLGVVRPKPSAEET
                     SD"
     gene            complement(1997..2078)
                     /locus_tag="PKI05_00620"
     tRNA            complement(1997..2078)
                     /locus_tag="PKI05_00620"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2042..2044),aa:Leu,seq:tag)
     gene            complement(2240..3241)
                     /locus_tag="PKI05_00625"
     CDS             complement(2240..3241)
                     /locus_tag="PKI05_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOA50725.1"
                     /translation="MTESVDHAATATPLHGVYQIIAEEMGEVDRLLRHELQDSHPYVD
                     RMLKHGLQLGGKRLRPALLLLAGKICGDINQTHIKLATAVELVHTASLIHDDVLDDAR
                     MRRHMATMNALWDNEASVLLGDYLLARAICLASSVDSVYACQVIAQASHEMCQGELRQ
                     IATRGKFDISEEEYIDIVAQKTASLYRACCRLGAYYAGATSRVVNDLEQFGTDLGIAF
                     QIIDDILDVLGDERRAGKSLGSDLDKQKPTLPIIRLLSVGNEDQRKELLTLLREQPEG
                     YRNRIRQMLAEQDAIPYAQDRAKGFVIAAMGRLSHFTPSPVLEAVRQLATFVISRDH"
     gene            3664..6651
                     /locus_tag="PKI05_00630"
     CDS             3664..6651
                     /locus_tag="PKI05_00630"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50726.1"
                     /translation="MRTWRPTRCFRCLVPAVLPVFLCTYMISGESCWGAVRIEKVITN
                     TCGEVRATSETKFGFYVFAGDDGAQLAVMPSAGTILAVLPARTHVTLSLRVSGSNEAE
                     KWELAVTSRVAGAAHVSVSFGQTTLSSRVTQDKPWTLPLPKGDPSELCEVTINPESDT
                     SRAVLLLELRNLGQLPQGDLIELTPGDPQVTPPPVCPNLHPEIQTAMIEWDWRQQDAL
                     DSCSPSLEAVHKAIARLIQQGDVLVDDLSQMGVQSDLFAQWRDVCGKWRNDDFQKDVA
                     SDVRAQLAVWQRIRTLKRQIALLNPYLAGKKLVFVKHVPSMFSHQLTQYTGNCARPGG
                     GLFVLEEPGRSMLARNLISGNFPLGSYQFCDVSPDGKKVLFSFCEVPSAPPDREAALD
                     RFFHLWEMDWDTRACRRLTDGSFDHFAGKYLADGRIVCVSTRRGGFHRCGRGPCPVHT
                     LTLLERDGSNARVISFHETHEWDPVQLLDGRILYTRWDYVDRNAVFYQQLWTVRSDGT
                     YVQAYYGNNTFNPVGIWEARPIPGTHAVMGTAAAHHAMTAGSIIRLDVCRGIDGPEPI
                     TRLTPDALFPESEVPVINRSGGRWFNPVGVTPPPLPEEQKRFPGHCYRTPFPLSEKYF
                     LVAYSYDPLIGEPDANQVNMFGIYLMDVFGNKELLYRDLNISSLWPVVVGNKPGTQPG
                     EAKVAVHLANHIIAGADEVASQSGRVSPTNESSPQAEGEFLLTNVYTAWPRLPPVKIT
                     RLRIVQVLPKSTWHANDPPLGLPNISPGKQVLGTVPVEPDGSAYFRAPAGKALSFQAL
                     DEMGQAVQIMRSVTYVQPGEIASCIGCHEPRNTAPAISHLPLAATRPPSEITPGPPGS
                     RPLSFPILVQPVLDRHCVRCHGENEPGGGIVLSGVPQGRYTKAYLALAPQVPYADWAG
                     RPGDFRVVNSEPVTQPDFFGARGSKLLSMLLEGHYDVHLDQDDLERLVTWMDCNALFY
                     GTFNPEDQKRQQKGEVILGPALE"
     gene            7113..8336
                     /locus_tag="PKI05_00635"
     CDS             7113..8336
                     /locus_tag="PKI05_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HOA50727.1"
                     /translation="MIRKVCDDGVGYSVVDLTDVRHVFAAAVPRCTGDLTTQTWDALR
                     TIQAVMKEESTLGSIVHQVVFLAEYDKVDECHEVMREFYGADFPATTYVPQKPCQGGI
                     VSIEALGVGRKRGDVVIDRRNEQLVIASHNNVSWVHCANIVPDPGIDGVYHQALNAFQ
                     KMNDMLGRAGVRFDQVIRTWLYLGGIVEGDGEGQRYKELNRARTDFYQGIPFLSDRLP
                     VHLKEAYPASTGIGADGHYLIMSCIALQTPRTDIVAMPLENPRQTPAFGYGCQYGIKS
                     PKFSRAMALSCGAYATIFISGTASIINSETQHIGDVERQTDETLDNIAALISEENLSR
                     HYLPQLGTTLDQLALVRVYIKRAEDYPKVRAICERRLGNVPTIYAVADVCRPELLVEI
                     EGIAFSQRGCQSGQC"
     gene            complement(8389..>8816)
                     /locus_tag="PKI05_00640"
     CDS             complement(8389..>8816)
                     /locus_tag="PKI05_00640"
                     /inference="COORDINATES: protein motif:HMM:NF019938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1731 domain-containing protein"
                     /protein_id="HOA50728.1"
                     /translation="RFGQVLARQGGVVASLLPVFRLGLGGPIGQGLAWWSWILLEDVC
                     RAVEFLLDHAEIRGPVNITSPNPVRNRDFTKAFAKAIHRPAVLRVPTLLLKLLFGQLA
                     QEVLLASARVIPVRLQESGFSFAYADIDKAFAKIFETVL"
BASE COUNT         1984 a         2300 c         2518 g         2014 t
ORIGIN      
        1 cacaaaacct gggtaaacaa ccccttcgat tggaaaaccg cgagcaccca ccgtccccct
       61 aaaagcggta aaccggatct atcatgcttt atcttacttt gcacccgccg agatggcaac
      121 gagattggaa ccctctgaaa gtggggtttc gatggaaaat gtcggctctg cccctatgca
      181 agagcggtgg agtttcgccc cccttgcgtg aacgtttctt agccgtataa tgccaacaaa
      241 gagagaggcg ataacgaaaa tcgatcgatg cgggtgtagc tcaggtggta gagcacgacg
      301 ttgccaacgt cgttgtcgtg ggttcgagtc ccatcacccg cttttttact ttgtaggttt
      361 gggtgattta gatggtcagg ggtttccatg gttgcctcgg atggtttccg tcaagacgac
      421 gaagaattga agcaggcttc cggcctgagc gaagtcgaag atgcggattt gatggcgcct
      481 gccgccacag atgaatcggc gggcgaagcg gaagaaagca cggagacacc actcgagttc
      541 gagttcgatg tggaggctgc cggtccctgc aagcggaaaa tcatcgtgac gattccgcgg
      601 aaggaggtcg atcgctattt ccaggaagag ttcgataaga ttcgggacga ggcacaagtt
      661 cctggtttcc gtcccggtcg ggcacctcgg aaactcttgg aaaagcgcta tcgtaatgag
      721 ctgcgcgagc cggttaaagc gcgtttgatg ctggacgcct ttaccgccct tgcggatcgg
      781 gcaaatcttg ccccaattag cgatcccgag ttggaccccg accggattga attgccggac
      841 gaaggtgatt ttgcatttga gttttcggtg gaggtacggc cggagtttga aatccccaat
      901 tggaagggcc tcaagctcac caagacggtt tactcctaca cacctcagta ctttgaagcg
      961 tacttgcgcg agcagcttaa gagtgaggcc ttgctcgaag aggtggaaga gccggcccaa
     1021 ctgggcgatt atatcacctg tgacatatcc ctctttcacg aaggtcgcgt cttaaactcc
     1081 tccgatggtg agcgattgtt gatccggcct cgtttgacat tcatggatgg tgtgatcgag
     1141 gatttcgggg agaggatggt cggcgtccaa cggggcgagt cgcgtacctg tgacgtgcgg
     1201 gtggcggagt cttgttctga tcctgagctt gccggcaaga caattcaagc ccggtttaaa
     1261 gtccacgcgg tactgcgacg gcggagtgct gagaatctag aagcgtttgc ccagacgatg
     1321 gggtatggct ccgtcgagga gttgcgggat gcttacgagc ggcgtctccg ctctcgctta
     1381 gatcaaatga cgtacgagag tctgcgtaag cagattctcg atcaactttt ggatgtggtt
     1441 ccatttgatt tgccccagga tctcgtcaag cgccagacgg agcgagagct tcgcaggcgg
     1501 gctgtggagc tggttcgggc cggttattct caagaggata tccaaccgca tctcaatgcg
     1561 atcgcgcggg aggccaggac ccaagtcgtt cgtctgttga gagaacattt cctgcttgaa
     1621 cggttggctg aagcggagaa tatcgaagcc gacgaggacg ctattgacgc ggaagtggag
     1681 cgaatcgcga tgcaaaccgg tcgctctcca cggcaggtcc gcgcggagct ggatcaagaa
     1741 gaggcttggg atgtcatccg taatgccatt gtcgagcgga ggatgattga aaaaattatc
     1801 gaggtagccg agatcactga ggagcagaag acgttgaccg ctcgcgagga agccgaagaa
     1861 acggccctcg acctgggagt agttcgaccg aagccaagtg ccgaggaaac atcagactga
     1921 gacaaaagat gcgtccaagc caaactcgtg agaccgccct tagtggcggt cttttttatt
     1981 cagtggcttg aaagaatgcg gacggaggga ctcgaaccct caccccttgc gggacaagat
     2041 cctaagtctt gcgtgtctgc caattccacc acgtccgcac aactaagacg ttggataggc
     2101 ctaaccgtca acttttctgt cgaccctgct ttgattctac taagttcccg cgaccgttgc
     2161 cagatcggtc gaaggcacca gaaagccgcg caattacgcg ttgcgtgaac acgccgtcga
     2221 gcagaaacgg cttccaagat cagtggtctc gtgagatgac aaaggtcgcg agttgtcgga
     2281 cggcctcgag aactggactg ggggtgaaat gagacagtcg ccccattgca gcaatcacga
     2341 aacctttcgc acgatcttgg gcgtagggaa ttgcgtcttg ttctgcgagc atttggcgaa
     2401 tacggtttcg gtagccttcc ggctgctcac ggagcaatgt caacaactct tttcgctggt
     2461 cttcattacc cacggacaac agtcgaatga tgggcagcgt tggcttctgt ttgtccagat
     2521 cactacccaa ggattttcca gcccgccgtt catcacctag cacgtcaagg atatcgtcga
     2581 tgatttgaaa ggcgattcct aagtcggtac cgaactgctc aaggtcgttt accacacggg
     2641 aggtcgcacc ggcataatag gcgccgaggc gacagcatgc ccgatacaac gaggccgtct
     2701 tttgtgcaac gatgtcaatg tactcctctt cggagatgtc aaactttccc cgggtggcaa
     2761 tttgtcgcaa ttctccctga cacatctcgt gactggcctg ggcaataacc tgacaggcat
     2821 aaactgaatc cacggaactg gccaggcaaa tggcccttgc caggaggtag tcgcccaaca
     2881 ggacgctggc ctcgttgtcc cagagggcat tcatggttgc catgtgccga cgcatccggg
     2941 catcgtccaa aacgtcatca tggatgagac tggccgtgtg gacgagctcg acggctgttg
     3001 ccagtttgat gtgcgtctga ttgatatcac cgcagatttt tcccgcaagc agaagaagag
     3061 ccggccgcag gcgtttgccg cccaactgca agccgtgctt gagcattcga tcgacgtagg
     3121 gatgagaatc ttgtaattcg tgacgcaaca agcgatcgac ttcgcccatt tcctcagcga
     3181 tgatctggta aacgccatgt aaaggcgtgg ctgtggcggc atgatcgacg ctttctgtca
     3241 ttcggccatc ctttagaaac acctagattg attctcttct gtcgctatct gattttttat
     3301 aggcgcaagt cttcgtggcg tgccggttct tccggtttta caggcatgcc acaatcccac
     3361 tcaatttgtt tcatcgacac atttagtgta cttgcaacga ttaagcgctg gcaatcgctt
     3421 atgggaccgt tttgttcgac ggcaagcggg ggtcgcaaga accggcttga gccaagcggc
     3481 gtcccaaact cactacttct ctcttcttta ctactgtcag actgtcaaaa ctgtttgatc
     3541 ttcgccacca gaagtggtag catcttggtt aacgaccacc tgttggcggc ccccaaagaa
     3601 tcgaaagctc aactttgccg gctctagcgg tattggcaat acccatgtga ggagtgggaa
     3661 ccaatgagga cgtggcggcc tacgcggtgt tttaggtgtc ttgtgcccgc tgtcctaccg
     3721 gtgttcctct gcacctacat gatttcgggc gaatcctgtt ggggcgccgt tcgaattgaa
     3781 aaggtcatca caaacacatg cggcgaagtg cgggcgacca gcgaaacgaa attcggattc
     3841 tacgtctttg ccggggacga tggggcccag ttggccgtga tgccgtcggc ggggacgatt
     3901 cttgccgtcc ttccagcacg aactcatgtc acattgtcgc ttcgtgtctc gggttcgaat
     3961 gaggcggaaa agtgggaatt ggctgtgact tcccgcgttg ccggggctgc ccacgtttcc
     4021 gtgtctttcg ggcaaacgac tctttcttcg cgggtcaccc aggataaacc gtggacgctg
     4081 ccgctgccga aaggtgatcc ttcagaactg tgtgaggtga cgataaaccc cgaaagcgat
     4141 acgagcagag cggtgcttct tttggaacta cgaaaccttg ggcagttacc acaaggcgac
     4201 ctcattgagc tgacgcccgg tgatccgcaa gtgactcccc cgcctgtgtg tcctaatttg
     4261 catccggaaa tccaaaccgc catgatcgag tgggactggc ggcagcagga cgctctggat
     4321 tcatgttccc catcgttaga ggcggttcat aaagccattg cgagactcat ccagcagggt
     4381 gatgttttgg tagatgatct ctcgcaaatg ggggtgcaat cggatctatt tgcccagtgg
     4441 cgggacgtgt gcggaaagtg gcgaaacgac gatttccaga aggacgtggc atcggacgtt
     4501 cgtgcccaac tggccgtgtg gcagcgcata cggacgttga aacggcaaat tgcgcttcta
     4561 aatccttatc ttgctgggaa gaaactggtg tttgtgaagc atgtccccag catgttcagt
     4621 caccagttga cgcagtacac aggcaattgc gcgcggcccg gtggggggct ttttgtgttg
     4681 gaagaaccag gtcggtcgat gttagcccgt aacctaattt cgggtaattt tccgctggga
     4741 agttaccagt tctgtgacgt ttcacccgac ggcaaaaagg tgcttttttc tttctgtgag
     4801 gtgccatctg ccccacccga ccgtgaggcg gcccttgatc gctttttcca tttgtgggag
     4861 atggactggg acactagggc ctgccgacgt ttaaccgatg gatccttcga ccattttgct
     4921 ggcaagtatc tggccgacgg tcggatcgtt tgcgtttcga cccgtcgggg tggtttccat
     4981 cggtgtggtc gagggccttg tcctgttcac acattaacgc ttttggaaag ggatggctcc
     5041 aacgcgaggg tgatttcctt ccacgagact cacgaatggg acccggttca gcttttggat
     5101 ggccggattc tttacacacg gtgggattat gtagatagga acgccgtctt ttaccagcag
     5161 ctttggactg ttcggagtga tggcacatat gttcaggctt attacggtaa caacacattc
     5221 aaccccgtcg gtatttggga ggcccgaccg ataccgggca cacatgcggt gatgggcacg
     5281 gccgccgccc atcatgccat gaccgcggga tcgatcattc gcctcgacgt atgccgggga
     5341 attgatgggc ccgagccaat aactcgccta acaccggatg ccctttttcc ggagagtgaa
     5401 gtccccgtga tcaatcggtc gggcgggcgg tggttcaacc cggttggggt gacgccccct
     5461 cccttgcccg aagaacagaa gcgatttccg ggtcattgct accgaacgcc atttcccctg
     5521 tcggaaaaat acttccttgt tgcctacagt tatgacccgt tgattggcga accggatgcc
     5581 aatcaggtca acatgttcgg catctatctg atggatgttt ttggtaataa ggaattgctg
     5641 taccgcgact taaacatctc gagtctctgg cccgtcgttg tcggaaacaa gcccggtacg
     5701 caaccgggtg aggctaaagt ggcagttcat ttagcgaatc acattatcgc gggtgcagac
     5761 gaggtggcat cacagagtgg acgagtctct ccaacgaatg agagctcacc tcaagcggag
     5821 ggagagtttc tattgaccaa tgtttacacg gcatggccac gactaccacc ggtcaagatc
     5881 acacgactgc ggattgtcca ggtgctgccg aaatcgacgt ggcacgccaa cgatccgccg
     5941 ttgggactgc ccaatatctc tccggggaag caggtcttgg gtacggtccc cgtggagccg
     6001 gacggctccg catattttcg ggccccagcg ggaaaggctt tgtcctttca agccctcgac
     6061 gagatgggcc aggcggttca gatcatgcgg agtgttactt acgtccaacc aggcgaaatc
     6121 gcttcttgca tcggttgcca tgagccgcgg aataccgcac ctgccatcag ccatcttccg
     6181 cttgcggcaa cacggccacc gtcagagatc acacccgggc ccccgggctc gcgaccgttg
     6241 agctttccga tcctcgttca gccggtgctc gaccggcatt gtgttcgatg tcacggagaa
     6301 aacgaaccgg gtggcggcat cgtcctcagt ggggttccac aagggcgtta tacaaaggcc
     6361 tatctcgcgc ttgctcccca ggtgccctat gccgactggg caggccgtcc cggcgatttc
     6421 cgggttgtca actctgaacc tgtcacgcaa cccgatttct ttggtgctcg aggatcaaag
     6481 ctcctgagca tgctcttaga agggcactat gatgtccatc tagatcaaga tgacctggaa
     6541 aggctggtaa cctggatgga ctgcaacgcc ctgttttatg ggacattcaa tcctgaggac
     6601 caaaaacggc agcagaaagg ggaagtcatt ttgggaccgg cattggagta gtgccagtgc
     6661 gctgtgaagg gaaagcgcta ttctaccttt ggagggagtg ggcaaaaaac ggagaagatt
     6721 gttgcaaatg cgatatgatg cggttagact gtaaacaaca tcgtagtttt tgccttcatg
     6781 ctgacccccg catttgcgac acaagaatcg gaacaaattc ctaccttgga ttacctcggc
     6841 catttgttca gggaagttgt cattttttgc aacgcaacag tgtaaataat agtggccgct
     6901 tggtgttttg atgccgcacc gagattacaa ctttcacgcc gcgggctgcg gacggatcgt
     6961 gcgtcagtga cgaatcgcca cctgttgctt tgtgaacact gtgttaacaa agggcgaatt
     7021 cccgcaacgt tcgggcgtaa aacctggtgc gaacccaata acctttagca gaaggcattc
     7081 tcacggtgac cctccaaacg cgagaacgca gcatgattcg gaaagtctgt gatgatgggg
     7141 tcggctattc ggttgtcgat ctcacggatg tccgacacgt gtttgccgcg gctgtgcctc
     7201 gttgcaccgg ggacctcaca acgcaaacgt gggatgccct tcgcaccatt caggcggtaa
     7261 tgaaagaaga gagcacatta ggctccatcg tccatcaggt ggtcttcctt gccgaatacg
     7321 ataaggttga cgaatgccac gaagtaatgc gggagtttta cggggcggac ttcccggcaa
     7381 cgacgtatgt gccgcagaag ccctgccaag gggggattgt ttccatcgaa gcactaggcg
     7441 tgggacggaa acgtggcgat gtcgtcattg accgacgaaa tgagcagctt gtgatcgcgt
     7501 cgcataacaa cgtctcatgg gtgcactgcg cgaacattgt gcccgatcct ggaattgacg
     7561 gggtctatca tcaagccttg aacgccttcc agaagatgaa cgacatgttg ggaagggccg
     7621 gggtgcgatt cgatcaagtc attcgcacgt ggctatatct gggtgggatt gtcgaaggag
     7681 acggcgaagg acagcgttac aaggaactca atagggcccg cacggacttt tatcaaggaa
     7741 ttccatttct ttccgaccgt cttcccgtcc atctgaaaga ggcctatccc gccagcactg
     7801 gcattggggc cgacgggcat tatctcatca tgagttgcat tgcccttcag acgccgcgga
     7861 cagatatcgt cgcaatgccg ctcgaaaacc cccgacaaac accggccttt ggttacgggt
     7921 gccagtacgg gatcaaaagt cctaagttct ccagggcaat ggcgctttcc tgtggggcgt
     7981 atgcaaccat tttcatttcg ggcactgcaa gtatcatcaa ctccgaaaca cagcatatcg
     8041 gggatgtgga acggcagacg gatgaaacgc tggacaacat tgccgcactg attagcgaag
     8101 agaacttgag ccgccactat ttgccgcaat tgggcacaac attagaccag ttggcacttg
     8161 tgcgggtgta tatcaaacgg gctgaggact accccaaggt ccgcgcgatt tgcgagcgaa
     8221 gacttgggaa tgtccccacc atttacgccg tggccgacgt ctgtcgtccg gaattactgg
     8281 tggaaatcga ggggatcgcg ttttcccagc gagggtgtca atcgggtcaa tgctaaaaaa
     8341 tcttgtcggg taaagcagga acccctcaca ccggcgatga tcggctgttc acagcacggt
     8401 ttcgaaaatc ttggcaaacg ccttgtcaat gtctgcgtag gcgaaggaaa atccggattc
     8461 ttgcaatcgt accggtataa cgcgggcact tgctaaaagc acctcttggg caagctgtcc
     8521 gaaaagcagt ttcaacaata atgttgggac gcgaagaact gccggacgat ggatcgcctt
     8581 ggcaaaggcc ttggtaaagt ctcggttacg caccggattc ggactcgtta tgttcacagg
     8641 gccgcgtatt tcggcatgat ccaggagaaa ctccacggca cgacaaacat cttcgaggag
     8701 aatccaactc caccatgcga ggccctggcc gatcgggccg cctaagccaa gacggaacac
     8761 cggcagaagc gaggcaacca cacccccttg ccgggcaagg acttgtccaa accgca
//
LOCUS       DAOPTR010000016         8804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_6628_length_8804_cov_4.611613, whole genome shotgun sequence.
ACCESSION   DAOPTR010000016 DAOPTR010000000
VERSION     DAOPTR010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 8804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8804
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6628_length_8804_cov_4.611613"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..570)
                     /locus_tag="PKI05_00645"
     CDS             complement(58..570)
                     /locus_tag="PKI05_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50729.1"
                     /translation="MENPYKPPTAQLRWEPSHPGSLVKAVIAGVLIDLGGTWIVNFVG
                     LAAYAFYLYSQGWSEDQVSREYVFLFIYSFPGLILPTARFGMSIVAGCICAKIAKRRR
                     LLAAILVSLTSALFGYLVLKNAYSYYAWREHLWLTALCSPALCLGAYLYARGRHPRKR
                     KVQATWTMTD"
     gene            complement(655..1875)
                     /locus_tag="PKI05_00650"
     CDS             complement(655..1875)
                     /locus_tag="PKI05_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOA50730.1"
                     /translation="MKATCFILFFFLMLIASIAETSATAFTGIGFLNHDGSSPSFARG
                     ISADGTTVVGMAINPLSTSGGDAFYWTRETGIVGMGYSSEGEALLSAWGASGDGSRIA
                     GQSTSHAFVADTHGLVTLIEDFPGGDGYHVAYDISNDGKTIVGMGTSEVGYQAFRQVD
                     GGPLEPVGPLLSGSNPLSYAQAISGDGRVVVGGFTSNRAVPQTSAFRWTEQSGTEYLP
                     YLHSNDINNWAGDISYDGKVIVGRTSPNGGGTGEATLWTENSVRGLGFLPGNYQDSYA
                     HAVSGDGSIVVGSSRTSDIWSPNGQWIGLEAFIWTEQQGMRPLKEVLSIDYGLSNVLD
                     GWVLSDVTGISKDGTILTGNGIHFGIEEAWYADLSANSLPFDTEVPEPASLSLLLTGF
                     AAFRLKHRRYSKYF"
     gene            complement(2287..3237)
                     /locus_tag="PKI05_00655"
     CDS             complement(2287..3237)
                     /locus_tag="PKI05_00655"
                     /inference="COORDINATES: protein motif:HMM:NF037521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HOA50731.1"
                     /translation="MKNNQEIIEKVTARIIEQLEAGVKPWECAWDRSGGEFSIPRNAK
                     TKEFYHGINIPILWEAAARKGYTTNSWIGYDQAKQLGGHVMKGELGTHGIIYKPLVVD
                     DETEENGQRTIPMLKSFVVFNLDQIAGLEHLKAKPPKAEGFLPIVVADDLLKKSQARI
                     VEGGPRAFYRKLDDLITLPDRWRFQVAEDFYATGLHELGHWTGHEKRLNREWGKRFGD
                     NAYAFEELVAEMISAFLCAYLGLTGELQHENYISGWLKILKADYRALVKAASLAQKGF
                     EYICALVEGLKQPTAEEPPEPPQRSCVREATAQLKLLLAA"
     gene            complement(3736..4596)
                     /locus_tag="PKI05_00660"
     CDS             complement(3736..4596)
                     /locus_tag="PKI05_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50732.1"
                     /translation="MLTLLALTGAATSTQLGERIGLVAKALHGVLGKLEAGRLIAPVA
                     RVRLAISGRPQTVWSLLPLGARRAGLYFTQGLGREALVKGLLKTHFVLANPEGYYPIF
                     FADQVAFFEERGEKWPWQGKEGPVRVSAQIRIVEDPPEEKGRVHVGLAVATLREARPL
                     LEPQRMVWAKRPGYRLTLLATEEQLAGIQAIFNPLNDWHDPYLQQQKTYREWRAILRG
                     LAPEEERYRHHIERVLKWVEMDGTQESAERHDLVMRAFPEPSQQPEMLTLFLGPIQLL
                     QGLGASNKPL"
     gene            complement(4614..7007)
                     /locus_tag="PKI05_00665"
     CDS             complement(4614..7007)
                     /locus_tag="PKI05_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50733.1"
                     /translation="MERFDDLTRRISRALKTPGQVRAAERAQWERRQARLPEYCARDL
                     LYDSRGLFTPADELSRMDAFLARHGRLVTDWQRKSPKLVIDLRAARRQLEQQIRTEGL
                     SFLERALLRRNFRATVSLLQTQGARRSYIGGTLTLNWRQKALYRFGELKPGTRPGTGF
                     KANGRTEAIFQEAKRQSGREGESLRYDPRRDPRLGVYPELKRLQQAGLARPDPPGVWK
                     LDRGLVEKKIREARNSRSLLSVSTLKRLRETRWQQSKDSGLKTVSAPDTQIRQALVEL
                     ESCYPQAARTTREGHQVNAPQLRKAIYADRTRFVEVWAKEHHAPPVIARCDHRLGEAL
                     AASTVPAHPDQGNLAVYHPEHHDTTGKAAEIARYLRGLAMRRDQNAVRFGQGGSFLID
                     RQEAVTLLKGHRAALLAYESRLGEAAKGGSPRVVSAFTHAGSPQPDGSWLLVPKAGES
                     ALLWRLHQEAALNPAAIGQEPDGSYRLSPDTARAHALPGTPEWGERMTTRYAGLDARV
                     AGIARPDFLSVTLKDERAPLSGKLAATTQATYERVARNQRQIPLEERPRYLIKRAQEL
                     SRCAWWLEHGVMRRELRALELGAGQNEAIRQACYELRTGIEAMAYQALRGGEGPRPIQ
                     AARKPVSPQALRELKAVIRARGDQELLDALVISEATGLRPGELAQGVRLEERGRSVYV
                     HIQGGKRHQGTAQAEARYQQARGADRVVEVKSQEVLEIARRHHGHFRPESSPDALRIR
                     LREARAEVPGGEHIQFYSFRHAIKNRLETEGHSREEIARLMGHLSTRSQEHYWADSK"
     gene            complement(7032..7283)
                     /locus_tag="PKI05_00670"
     CDS             complement(7032..7283)
                     /locus_tag="PKI05_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50734.1"
                     /translation="MQVVMNFLGLHLLTQGMLLAAFFLCRKQILAVYALWEYDQTLFC
                     FYVAGIGTLIWLVWTWIRRYVLFRSVGGKAGEASRTRGF"
     gene            complement(7305..8156)
                     /locus_tag="PKI05_00675"
     CDS             complement(7305..8156)
                     /locus_tag="PKI05_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50735.1"
                     /translation="MLKSKVFLMNPVMAISFALIAPLALANQPPATTPPTAPPVTPAP
                     AVTPPAAPAPTVAKASPNYQAGSVVTIYQLQDQGRKKGGFQQGQPIGSFVSEANPWNL
                     GLHKKQVDLAFFQGEPLGYELDAYFVVKEVGSYSFGAEVFLPPAEVFTDPDHEKGGDR
                     GWVECRYRLNVAGEVVIDMEASTRARGTPDEERKCGLTGHGFGSVQLDAGLHRVRQWF
                     ACTGERRIKMPQKQYVYPASCPNAGKRFSTDNFPADEAQVTLRVRHPQENTPVLVKTS
                     ELIHEKR"
     gene            complement(8183..8527)
                     /locus_tag="PKI05_00680"
     CDS             complement(8183..8527)
                     /locus_tag="PKI05_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50736.1"
                     /translation="MWRRQVVVEKVIIPILRALKDPREQIWFGIALATLLAAIFSSSG
                     GGMSAPTTASAKAQVASAQATAPHQTPHSGPFALFKPGPKKLIPGSRPLDSGTLPEEP
                     TKPHQDDFGVIH"
     gene            complement(8533..>8804)
                     /locus_tag="PKI05_00685"
     CDS             complement(8533..>8804)
                     /locus_tag="PKI05_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50737.1"
                     /translation="AAVLTERHGMELEEIEAEVVVEAIRVAQAGESTGARDFKRWVEQ
                     WVNPALLEAKRQRAWKVKLLLGPDRKVYATITAYRQERQPSPQRA"
BASE COUNT         1802 a         2597 c         2427 g         1978 t
ORIGIN      
        1 tggtagcaaa cccggtccgt gatttgaacc acactccatc aagccggaat tccccgctta
       61 atccgtcatc gtccaggtcg cctgcacttt cctctttctg gggtgtcgtc cacgggcata
      121 gaggtatgcg ccaaggcaaa gggccgggga gcacagggcg gtgagccata gatgctccct
      181 ccatgcgtaa tagctgtagg cgttttttaa cacgagatag ccgaacaaag ccgatgtgag
      241 gctgacgagg attgcggcca ataaccttct tctcttggca atcttcgcgc aaatacaacc
      301 cgccactatc gacataccaa aacgagcggt aggcaagatt agaccaggga acgaatagat
      361 gaagagaaaa acatattctc ttgatacttg gtcttcgctc cacccctggg aatacaaata
      421 aaaagcgtac gctgcaagtc ctacaaaatt gacgatccac gtgccgccaa ggtcaatcaa
      481 gacgccagcg ataacggcct tcaccaatga accggggtgc gacggttccc agcgtagttg
      541 ggccgtagga ggtttgtagg ggttttccat cgggtcctcg atctccacca gcgcgtaaac
      601 cgcttgcgca ggtgccttga gactatatcc ggggcgctaa tcttggcgca gcagttaaaa
      661 atatttagag tatcttcgat gtttcaatcg gaatgccgcg aatccagtga gcaacagtga
      721 caatgatgcg ggttcaggaa cctctgtgtc aaatggtaaa gagtttgcgg agagatccgc
      781 ataccaagct tcctctattc cgaaatggat tccgttaccg gttagaatgg tgccgtcttt
      841 agaaatacca gtgacatcgc ttaacaccca tccatcaaga acattactca aaccataatc
      901 tattgaaaga acctctttta gaggacgcat cccctgttgt tctgtccaaa taaacgcttc
      961 caaacctatc cattggccat tgggggacca gatatcagag gttctgcttg atccgaccac
     1021 aatcgatcca tctcccgaaa cagcatgagc ataactgtct tggtaattac ctggcaaaaa
     1081 tcccaaacct ctaaccgaat tttcggtcca tagcgtagct tcccctgttc caccaccgtt
     1141 tgggcttgtc ctacctacta ttactttgcc gtcgtaagat atgtcgccag cccaattgtt
     1201 gatatcattt gaatgtaagt aggggagata ttctgtgccg gattgctcag tccaacgaaa
     1261 tgctgacgtt tgaggaacgg ctcgatttga cgtaaaacct ccaactacga cgcgcccatc
     1321 accggaaatt gcttgagcat aagacaaagg atttgacccc gatagtagtg ggccaaccgg
     1381 ttccaaaggc cccccgtcca cttgcctgaa tgcctggtag cctacttcac tggttcccat
     1441 gccaactata gttttaccat cattggaaat atcatacgca acatgataac catctcctcc
     1501 tgggaaatcc tctataagag ttacaagtcc atgagtatcg gccacaaatg catggctagt
     1561 tgattgtccg gctatccttg aaccatctcc gcttgccccc catgcagata aaagagcttc
     1621 tccctccgaa gaatatccca taccaactat tcctgtctcc ctagtccagt agaaagcatc
     1681 ccctcctgag gtgctaagtg gatttattgc cattcccaca acagttgtcc catcggcgga
     1741 aattccccta gcaaagctcg gagacgaccc gtcatggttc aaaaagccaa taccggtgaa
     1801 tgcggtagca gatgtctctg ctattgaagc aataagcatt aaaaaaaaga ataggataaa
     1861 gcaagtcgct ttcatgagta aggccttcta gtttgtgttt gttaagctca aagtatcgtt
     1921 ttttagtgct tgcaaaattt ttccttcagt tctccgagag ttatggtgag atctctcaaa
     1981 taagcaggtt aatcctataa cacaggactt cgcgtaaaca aatttaaaat agagccttat
     2041 ctttattggt atacttgaat gttagatagg ataataagaa caagatgaga ttaaggagat
     2101 tcgtaattgt tcaaggtatt gttcaaggtt ttttggatta ctcgcctttt tgttcgtaat
     2161 caaaaaggaa tcgtggctca ccttcttagt cgtatcagcg gccccccacg ctacgcgctc
     2221 aaaccctccc ctgccagtga gcccaagtcc ttggactcac tgactgaaaa acctcttggg
     2281 taaccttcac gccgccagca acagtttcag ttgcgctgtg gcttcccgaa cacaggaacg
     2341 ttgcggcggc tcggggggtt cctcggcggt tggctgcttg aggccctcca ccagggcgca
     2401 aatgtactca aaccccttct gggcaaggct tgccgccttg accagcgccc ggtagtcagc
     2461 tttgagaatc ttgagccagc ccgaaatgta gttttcgtgc tgaagctcgc cggttaaccc
     2521 caagtaggca cagagaaaag cggagatcat ctcggccacc agttcctcaa acgcataggc
     2581 gttgtcccca aagcgtttgc cccattcccg gtttagccgc ttttcatgac ccgtccagtg
     2641 gcccaactca tgcagtccgg tggcgtaaaa atcctccgcc acctgaaacc gccagcggtc
     2701 gggaagggtg attaggtcgt ccagcttccg gtagaacgcc ctggggcctc cttccacgat
     2761 ccgggcttgg ctcttcttaa gcaggtcgtc ggctaccact atcggcaaaa agccctccgc
     2821 cttcgggggc ttggccttca agtgttccaa accggcaatc tgatccaggt tgaagacgac
     2881 aaacgacttc aacatgggga tcgtgcgctg gccgttttcc tccgtctcgt cgtccaccac
     2941 gaggggcttg tagatgattc cgtgcgtccc caactcgccc ttcatgacgt gaccgccaag
     3001 ctgtttggcc tggtcgtatc cgatccacga gttggtggtg tagcccttgc gggcggcggc
     3061 ttcccacagg atgggaatgt tgatgccgtg gtagaactcc ttggtcttcg cgttgcgggg
     3121 aatggaaaac tcgcccccgc tccggtccca tgcgcactcc cacggtttta cgccagcttc
     3181 gagttgttcg atgatcctgg ccgtgacttt ctcgatgatc tcttggttgt ttttcatgac
     3241 agacctccaa agcaggaggc ccgaacggaa aagaccggcc tcgtggcccg tcccttcggt
     3301 cgggccacgt ctcgaatcga accttttgta acgcagtagc atggtgctgc cgccttcacc
     3361 gtaagcggtc aaggcggtag cctccaaaca atccaactac tcttaatgca gggtgttatt
     3421 cagtcaattt atgcgggttt cgtgcaaaac tgcgccctga aaactggagg tttttggtct
     3481 cgccaagcgc gggcgaaacg ggggtttctg tggctgtttt ttcgcatttg caaaggagcg
     3541 ccaaaggcgc gaactttgta aatgcgcgct cgctagagcg ccagtcgggg cggggtcaac
     3601 cgccctgacg ccgggcgctt tgcgctcggc taaggcgtaa gccgcacaag gagaaagaaa
     3661 agcgatgtgg tttttttctg tagctagaaa gtttttgccg ctaatgcctg gattctttcc
     3721 gctcagaagt gacttttaga ggggtttgtt gctcgctccc agtccttgca acaactgaat
     3781 cggcccaaga aagagggtga gcatttccgg ctgctggctg ggttcgggga aggcgcgcat
     3841 gaccagatcg tggcgctcgg cgctttcctg ggtgccgtcc atctcgaccc acttgagcac
     3901 gcgctcgatg tggtggcgat accgttcttc ctcgggggca agcccgcgca agatggcccg
     3961 ccattcgcgg taggtcttct gttgctgcaa gtaggggtcg tgccaatcgt tgagcggatt
     4021 gaagatcgcc tgaatccccg ctaactgctc ctcggttgcc agcaaagtca ggcggtaccc
     4081 gggacgtttc gcccatacca tgcgctgggg ctctaagagg ggtcttgcct cgcgcaacgt
     4141 cgccacggca agcccaacat gcacgcggcc tttctcctcc ggggggtctt ccacgatgcg
     4201 aatctgcgcg ctcacccgga ccggaccctc cttgccctgc cacggccatt tctccccgcg
     4261 ctcctcaaag aacgccactt ggtcggcaaa gaaaatcggg taatagcctt cagggttggc
     4321 caaaacgaag tgggttttca gtaacccctt aacgagcgct tcccgcccca agccttgcgt
     4381 gaagtaaagc ccggcccgcc tggctcccaa gggcaataaa ctccataccg tctgcggcct
     4441 cccactgatg gccaaccgta cccgggccac gggggcaatc aagcgcccag cttcgagctt
     4501 ccccaacaca ccatgcaggg ctttggcgac aagtccgatc cgctcgccaa gctgggtgga
     4561 tgtagctgcg cccgtaagcg ccagcaaggt cagcacttct tggggagggt tcattattta
     4621 ctgtctgccc agtagtgttc ctgcgaacga gtggagaggt ggcccatcag ccgggcgatc
     4681 tcctcccgcg aatgcccctc cgtctccaag cggttcttga tggcgtggcg gaaggaataa
     4741 aactgaatgt gctcgcctcc tggaacctcg gcccgcgctt cccgcaagcg aatccgcaag
     4801 gcatctgggg aactctccgg gcgaaaatgc ccgtgatggc ggcgggcaat ctccaacacc
     4861 tcctgcgact tcacctccac cacccgatcc gctccccgtg cttgctgata ccgcgcctcg
     4921 gcctgtgccg tcccctgatg gcgctttccc ccctggatgt ggacgtaaac gcttcgtccc
     4981 ctctcctcca agcgaacccc ttgcgccagt tcccctggcc gaagtccggt ggcttcgcta
     5041 atcaccaggg catcgagaag ttcctgatcc cctcgggcgc ggatcaccgc cttcaactcc
     5101 cgtaacgcct ggggcgatac cggtttccgg gcggcttgaa tcgggcgggg accctccccg
     5161 cctcttaaag cctgataagc catcgcctcg atcccggtac gcagttcgta gcacgcttgc
     5221 cggatggctt cgttttggcc agcgcccagt tccagggcgc gcaactcccg ccgcatgacc
     5281 ccgtgttcga gccaccaggc gcagcgggac aactcttgag cccgtttgat gaggtaccgc
     5341 gggcgttctt cgaggggaat ctggcgttga ttacgggcta cccgctcgta ggtcgcctgg
     5401 gtggtcgcgg caagctttcc gctcagtggt gcgcgctcgt ccttgagggt taccgaaaga
     5461 aaatccgggc gggcgatccc ggctactcgg gcatcaagtc ccgcatagcg cgtcgtcatg
     5521 cgttctcccc actccggggt tccgggaagg gcgtgcgctc gggcggtgtc cggggatagc
     5581 cggtaactgc cgtctggctc ctggccaatc gcggcgggat tgagggcggc ttcctggtgc
     5641 aagcgccaaa gcaaggcact ttccccggcc tttggaacca agagccagct tccgtctggt
     5701 tgcgggctgc cggcgtgggt aaaggcggaa acgactcggg gggaaccccc tttggcggct
     5761 tcccccaagc ggctttcata ggccaggagg gcggcgcggt ggcccttaag caaggtgact
     5821 gcttcctgcc ggtcgatgag aaaactgccg ccctggccaa agcgaactgc gttttggtcg
     5881 cggcgcatgg ccaatccccg caagtaacgg gcgatctcgg cggccttgcc ggtggtgtcg
     5941 tggtgttcgg ggtggtaaac ggcaaggttc ccctggtcgg ggtgtgcggg aacggtgctt
     6001 gccgcgagcg cctcccccaa gcggtggtcg caacgggcga tcactggggg cgcgtgatgc
     6061 tctttcgccc aaacctccac aaaacgggtc cggtcggcat agatcgcttt tcttaactgc
     6121 ggagcattta cttgatggcc ttcccgcgtg gtgcgggcgg cctggggata gcaagattca
     6181 agctcgacaa gggcttgccg gatttgggta tccggggcgg ataccgtctt caatccggag
     6241 tcttttgact gctgccacct cgtttcccgt agccgcttca gggtggaaac agaaagtagg
     6301 gagcgcgaat tcctcgcttc ccggattttt ttctcgacta acccccgatc cagtttccaa
     6361 acaccggggg ggtcgggtct ggccagtccc gcttgttgca agcgttttag ttcgggatac
     6421 acccccaagc gcggatcgcg gcgggggtca tagcgcaaag actccccttc ccggcctgac
     6481 tggcgcttgg cttcctgaaa aatcgcttcc gtgcggccgt ttgccttaaa gccggtccct
     6541 gggcgcgtgc cgggctttag ttccccaaag cggtaaagcg ctttctgtcg ccagtttagg
     6601 gtgagcgtgc cgccgatata ggagcggcgt gcgccctggg tttgaagcaa gctcacggtg
     6661 gcccggaagt ttcggcgtag taaagcccgt tccaggaagg agaggccctc ggtgcgaatt
     6721 tgctgttcca gttggcgccg ggcggcccgc aagtcgatca ccagttttgg ggatttccgc
     6781 tgccagtcgg tgaccaaacg gccatgccgg gcaaggaagg cgtccatccg gctcagttcg
     6841 tctgccgggg tgaacagccc gcgagagtca tacagcaaat cgcgggcgca atactcggga
     6901 agtctcgcct ggcggcgttc ccactgcgcc cgctcggcgg cgcgaacctg gccgggcgtt
     6961 ttaagcgccc ggctgattcg cctcgtcagg tcgtcaaagc gttccatgac ttctcaaaat
     7021 ttctcaagaa gtcaaaaccc acgggtccgg gaagcctctc cggccttccc gcccacggaa
     7081 cgaaaaagca catagcgccg aatccacgtc caaaccagcc agatcagggt gccgatccca
     7141 gcgacataga agcaaaagag ggtctggtca tactcccaca aggcgtaaac cgccaagatt
     7201 tgcttgcggc acaagaaaaa cgccgccagc aacattcctt gcgtgagcaa atgcaggcca
     7261 aggaaattca taacgacttg catgatgatg acgctccccc cacttcagcg cttttcgtga
     7321 atcaactctg aggtcttgac cagaaccggg gtattttcct ggggatgccg gaccctaagc
     7381 gtcacctgcg cttcgtcggc ggggaagtta tcggtgctaa accgcttgcc ggcatttggg
     7441 caactggccg ggtagacata ctgcttctgg ggcatcttga tgcgcctttc cccggtgcag
     7501 gcaaaccact gccgcactcg atgtagcccg gcatcaagct gcacgctgcc aaagccgtgc
     7561 ccggtaagcc cgcatttacg ctcctcgtcg ggggttcccc gtgccctggt gctcgcctcc
     7621 atgtcgatca ccacttctcc ggccacattg agccggtagc ggcactcaac ccatccccgg
     7681 tcaccgcctt tttcgtggtc gggatcggtg aatacctcgg cgggcggtaa gaacacttcc
     7741 gctccaaagg aataacttcc cacctccttg accacgaagt aggcatccaa ctcatagccc
     7801 agcggctccc cctggaagaa cgccaggtcc acctgtttct tgtgaagccc caaattccag
     7861 gggttggctt cgcttacaaa actgcctatc ggctggcctt gctggaaacc gccttttttc
     7921 cggccttggt cctgaagctg ataaatggtc accacgcttc ctgcttggta attggggctt
     7981 gccttggcca cggtaggcgc gggcgctgcg gggggcgtaa ctgccggggc gggggttact
     8041 gggggtgcgg tcggcggggt ggttgcgggg ggctggttgg ccagcgccag cggggcgatg
     8101 agggcaaaag aaatggccat gacggggttc atcagaaaaa ctttgctttt caacatgatt
     8161 gctttctccg gtattcagga attcagtgga tgacgccaaa atcatcctga tgaggttttg
     8221 ttggctcttc gggcaaggtc cccgaatcga ggggacggct tcccggaatg agctttttgg
     8281 gacctggctt gaagagggca aagggtccgg aatgcggcgt ctgatggggg gcggtcgcct
     8341 gtgcgctcgc tacttgggcc ttggcgcttg ctgtggtcgg ggcactcatc cccccgccac
     8401 tagaggaaaa tatcgccgcc aagagggttg ccagcgcaat tccaaaccaa atctgctcgc
     8461 gggggtcctt gagcgcccgt aggatcggaa tgattacctt ttccaccacc acctgacgcc
     8521 gccacatggg atttaggccc tctggggaga aggttgccgc tcttggcggt acgccgtgat
     8581 ggtggcgtat accttcctat ctggccccaa aagcagcttc accttccagg ctctctgccg
     8641 cttggcttcc aacagtgcgg gatttaccca ttgttctacc cagcgtttga agtcgcgtgc
     8701 cccggtgctt tccccggctt gcgctacccg aatcgcctcc accaccacct cggcttcgat
     8761 ctcttccagt tccatcccat gccgctcggt cagaacagcc gcta
//
LOCUS       DAOPTR010000017         8683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_6765_length_8683_cov_3.917942, whole genome shotgun sequence.
ACCESSION   DAOPTR010000017 DAOPTR010000000
VERSION     DAOPTR010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 8683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8683
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6765_length_8683_cov_3.917942"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1461)
                     /locus_tag="PKI05_00690"
     CDS             complement(<1..1461)
                     /locus_tag="PKI05_00690"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOA50738.1"
                     /translation="MRNLPPAITRPNASMRRLAFLLIGTFFAHLGLFGVCEADDQPQW
                     GERFSRNMVSREKHLPADFDPATGKNIRWAVELGTNTYSSPVVAKGRVFIGVNNAKPR
                     DPRHQGDRAILMCLDEQSGELIWQLVVPRLGEDDPYLDWPRISMCSPPTVEGNRVYTV
                     TNRFEVVCLDLEGQANGNDGPYTDEGRHMVPPGEAPLEVTNLDADIIWLFDLPSQAGI
                     YPHDGAHVSILLDGRFLYLNTGNGVDNTHRKIRAPDAPSLIVLDKETGRLVAKDDEKI
                     GPRVFHSTWSSPALGVVDGQRLIFFCGGDGVVYAFRALPQDWQSTEVVPLERVWKFDC
                     DPTAPKENVHQYITNRKVSPSNIKSMPVFYRDRVYVTVGGDIWWGKREAWLKCINARG
                     KGDITSTHELWSYPLKEHCCSTPSIAGDLVFVADCGGFLHCVDAETGHAYWTHDLRGE
                     TWGSTLVADGKVYVGTRRGVFWTFAASREKQLLAEIN"
     gene            1869..3869
                     /locus_tag="PKI05_00695"
     CDS             1869..3869
                     /locus_tag="PKI05_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOA50739.1"
                     /translation="MEDPSQTEHFADKMTHRSIRREYDRSAKQVVWLVVLIWSSVILA
                     SLWGMFLADPMVRSIQGAVSLALLWSAGLGVIILFGYRWADYRTRIDQTMTRQIAFRL
                     FTRDTPAAVALVDAYGRFRLVSPAFCQLLGRTEKELIGQRAIRFAHPDDAAILREQYR
                     LRRRGQSSTYQLRVIRADGAVRWLLAAVWPVYDDKGRYCGGLGLVADITEQKQAEEEL
                     RQHTRELESLVQQLEKAQAEARAAALAKTEFLATVSHELRTPLTAILGYAEMLLLEGD
                     LTKIPANRLNAIQTILRNGEHLLQIINDLLEMAKLETGRLEPEVRDFSLPQLLHDVVR
                     LMQIRAESKQLALRLEICGPIPKMIRSDPLRIRQILINLVGNAIKFTELGSVRIVVQV
                     PRNEDGTCSFVCEVIDTGMGIPPEPCDKIFEPFHRGDPTSRRKFSGTGLGLTVSRFLA
                     RRLGGDITVVSEVGLGSNFRLVIPLGLADQYEWITCTADDLDVTQKSIREVQAVASAE
                     KGAPLTARVLLVEDCPDTQRLFTHILSQAGADVTSASTGRDTIRLAVQAMVEQRPFDC
                     LLLDMQLPDIDGYHVARMLRDSGYTAPIVALTANMTPGERERCLASGCDDFATKPIKR
                     QDLVNLVAKYAPQTGGLAPIPGEMSSRPSLTTGAPLPQPASN"
     gene            complement(3908..5167)
                     /locus_tag="PKI05_00700"
     CDS             complement(3908..5167)
                     /locus_tag="PKI05_00700"
                     /inference="COORDINATES: protein motif:HMM:NF016203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HOA50740.1"
                     /translation="MTTVTPGQELQASQGQTSGTAAQWEGYLRADGRKGIRNCLVVVY
                     LVECAHHVAAEIASPFANRGVELIGFPGCYPSEYAHKVLSRVTTHPNVGAVLLVSLGC
                     ESFDRARLRAEIAASGRPVELLVIQEIGGTARTIKAGRQWIKSVLPELAKVPRCPMEL
                     RDLVVGTICGGSDATSSLTANPACGLVFDRLVDSGATAIFEETGELLGCESLLAERAA
                     RPELADVLRACVEKAARYYATLGHASFAPGNTEGGLTTIEEKSLGAYLKSGHRPITGV
                     LKPGDLPPSPGLYLLDVVPDGEVRFGFPNIADNAEIVELIACGCHLILFTTGRGSVVG
                     SAISPVIKICANPETYARMKSDMDINAGEVLTGKSTLEEVAERIWQFMIAVAGGKQTV
                     SEALGHREFILTYKTFEPLGPACLPTV"
     gene            complement(5246..5809)
                     /locus_tag="PKI05_00705"
     CDS             complement(5246..5809)
                     /locus_tag="PKI05_00705"
                     /inference="COORDINATES: protein motif:HMM:NF020250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HOA50741.1"
                     /translation="MKPKLLRLHPRDNIAIATAPIKAETVLKLDRVKIQIISNVPMAG
                     KVATRPIAEGEKIIKFGQPIGHATRNIEPGEWVHTHNLASDYLPTPERPSPASDVSTQ
                     PVSEPVGENSAVGTSAVVETLSGPSAEGEISANSKAFSSDSLPSQDSPVVEEGPTPSP
                     TPNEPNEPKLEQGTSPAEIERSDSTVQ"
     gene            complement(6014..6412)
                     /locus_tag="PKI05_00710"
     CDS             complement(6014..6412)
                     /locus_tag="PKI05_00710"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOA50742.1"
                     /translation="MDKHSRRYFDRILEEVIEELPQLVRDAMDKVPLYVEDYPSRRVM
                     RQTGIRNRRELWGLFTGVPLNEKHFDAPSVTLPDRVTIYREGLLWSAVGRDGYVDEKR
                     LKKQIRITILHEYGHYYGMTEEELEQYGYG"
     gene            6624..7658
                     /locus_tag="PKI05_00715"
     CDS             6624..7658
                     /locus_tag="PKI05_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50743.1"
                     /translation="MLKSEVARNYPFLRPAFSRRGFVTFKFAEGVKPDEGFSLEAVFA
                     RTWGFCFGRVPASDWQVAANEVWAKVANIPVGWIHVWPRDTSPPGRSEPEKPWPAEIS
                     QIQQVLMEHAPTKAIPPNVSQESTPCPPGVWVLDCVVVEPTEWWIGIHRAGSLESRYP
                     GGRFPIVLPEYAVSRAYLKIEEALRWSGFKIPANARWAELGSAPGGASQALLDRGYEV
                     LGVDPAEMHPLVLSHPKFTHLRRRTPQAPRRAFRKVRWLAADINAAPNYTLDAVESIV
                     TYPQVNIRGMILTLKLPNWQLAENIPEYMERIRSWGYNIVKARHLWNNRQEVCVAALM
                     KPFRRKPSHS"
     gene            7937..>8683
                     /locus_tag="PKI05_00720"
     CDS             7937..>8683
                     /locus_tag="PKI05_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50744.1"
                     /translation="MEFATPCFHVSFYVAILVGWLLPSIGAGLEPGLYPSTRDYPFTR
                     AMAPDYPNLPSLPTPGDNSRSATVTSSRLEQAGDDAEDAIIEGPDMALESADSQSAGA
                     MVREEGTKPDDEGLEKQDVMEAPQSPDGPSAAPQKPNADGDSSTDKPSARRSTIDPTS
                     VGRPLAAGIAKLVAPEIESQMEGANITARFQQWRSYAASRLAATRADYTSSEINGMAR
                     LKWYEHLLLNPLNVPHETEEFTRLLHAGFTA"
BASE COUNT         1889 a         2453 c         2393 g         1948 t
ORIGIN      
        1 gttgatttcc gctaaaagtt gtttttcgcg agatgcggca aaggtccaga agaccccacg
       61 gcgcgtcccg acgtaaacct ttccatccgc gacgagcgtg gatccccagg tctcaccccg
      121 caggtcatgc gtccagtagg catgacctgt ttctgcgtcc acgcaatgga gaaatccccc
      181 gcagtcggcc acaaaaacaa ggtcaccagc aatcgacggg gttgagcagc agtgttcctt
      241 gaggggataa gaccatagct cgtgggtgga cgtgatgtcg cccttcccgc gggcattgat
      301 gcatttcagc cacgcctcgc gctttcccca ccaaatgtcc ccacccaccg tcacgtaaac
      361 ccggtctcga taaaacaccg gcatgctctt gatgttactg gggctaacct tccggttagt
      421 aatgtattga tgaacgttct cttttggcgc cgtgggatcg caatcgaact tccacacacg
      481 ttccagggga acgacctcgg tggattgcca atcctgcggc aaagcacgga aggcatagac
      541 gacgccatca cccccgcaga agaagatcaa gcgttgaccg tcaactaccc cgagtgccgg
      601 tgaggaccac gtggagtgga aaacccgtgg cccgatcttt tcatcatctt ttgccacgag
      661 tcggcctgtt tccttatcga ggacaatcag actcggcgca tccggcgcac gaatcttgcg
      721 atgcgtattg tccacaccat tacccgtgtt gagatacagg aatcgaccat cgagcagaat
      781 cgaaacatgg gccccatcgt gagggtagat gccagcctgg gaagggaggt cgaaaagcca
      841 gatgatatcc gcatccagat tggtcacctc taggggagcc tcaccgggcg gcaccatgtg
      901 acggccttcg tcggtatatg gcccatcatt gccattggcc tggccctcaa ggtccaaaca
      961 caccacttca aaccggttcg tgacggtgta aactcggttg ccttccaccg tggggggaga
     1021 gcacatcgag atacgcggcc aatccagata gggatcatcc tccccaagtc gaggaacgac
     1081 aagctgccag attagctctc cggactgttc atccaagcac atcaggatcg cccgatcccc
     1141 ttggtgacgt ggatcgcgcg gcttggcatt gttgacccca atgaaaacgc gacccttggc
     1201 cacaaccggg gaggagtacg tgtttgtccc caattccaca gcccatctga tgtttttgcc
     1261 cgtcgcggga tcaaaatcag ccggaaggtg cttttctcgc gagaccatgt tccggctaaa
     1321 gcgttccccc cattggggtt gatcatccgc ctcgcatacc ccgaacaacc caagatgggc
     1381 gaaaaaggtt cctatcaaca ggaaagctaa ccgacgcatg cttgcgtttg gccgagtaat
     1441 ggcaggagga agatttcgca tgacagcgac tctctgaatg tctgacacga actgatggca
     1501 ggaaaggctt ggggatcgtc tttggcttgt cgggaactcc cgtccacagg gtgcctttac
     1561 attctcacgt atccctcgcg ttttcgcaac tcctgtcaat tttcacagag gctgttggcc
     1621 gtgtaccttt gctcagtcct gcgcaacaca tcaactaacc ctgtgccaaa caatgcgcga
     1681 cggggttccg tcctgcggat tatgacaata ataccgaggc ggtaactaac ataaaactgt
     1741 gaaacacgac agaaggggta agcttttctc ggcccgagtc gttttgcaga agctatggtt
     1801 tcgtaggttt gggccccatc tgttatgttg tcttggccat ttgtttcagc ctggagtttg
     1861 catcggtgat ggaagatcca agtcagactg agcattttgc cgacaaaatg actcaccgct
     1921 ctattcggag ggagtacgat cgttcggcca agcaagttgt ttggctggtc gttcttattt
     1981 ggtcgagtgt gattttggcg agtctttggg ggatgtttct ggccgacccc atggtccgct
     2041 cgattcaggg ggcagtatcg ctagcactcc tttggtccgc aggtttgggg gtgatcatcc
     2101 tgtttgggta ccgctgggcg gactatcgca cccggattga ccaaacgatg acccgacaga
     2161 tagcctttcg gctctttacc cgggatactc cggcagccgt tgctcttgtg gatgcttatg
     2221 gccgtttccg gctcgtgagt cccgcctttt gtcaacttct cggtcgtacg gaaaaagagc
     2281 taattggcca acgggctatc cgctttgccc atccggatga tgcagcgatt cttcgtgagc
     2341 agtaccgact gcgacgccgt gggcagtcat cgacctatca actgcgggtc atccgtgccg
     2401 acggtgcagt gagatggctg ctggccgcgg tgtggcccgt ttacgacgac aaaggccgat
     2461 actgcggcgg tcttggcctg gttgccgaca ttacagagca gaaacaagcc gaagaggaac
     2521 tgcgacaaca cacacgcgag ttagaatcgc ttgttcaaca gctggaaaag gctcaggccg
     2581 aagcccgtgc agccgccttg gcaaagacgg aattcctggc tacggtcagt cacgaactac
     2641 gcacgccctt gacggccatc ttgggctatg cagagatgct tttactcgaa ggtgatttga
     2701 ctaagattcc tgcgaaccgg ctaaatgcca tccaaacgat tcttcgaaac ggcgaacatc
     2761 ttctccagat cattaatgat ctgctggaaa tggcgaagtt agaaactggc cgattggagc
     2821 cggaagtacg agacttctca cttccgcaac tgctccacga tgtggttcgc ctgatgcaaa
     2881 ttcgcgcgga aagcaaacag ttggccttgc gactggaaat ttgtggacca atccccaaga
     2941 tgatccgcag tgacccccta cgaatcagac aaattctgat taaccttgtg ggaaatgcta
     3001 tcaagttcac ggagttagga tcagtacgga tcgttgttca ggtgccgaga aatgaggatg
     3061 gtacctgttc ctttgtgtgc gaggttatcg atacaggcat gggtatcccg cccgagcctt
     3121 gcgataagat cttcgaaccg ttccatcggg gtgacccgac gtcgcggcga aagttctccg
     3181 gtacgggctt gggcctgacc gtcagtcgct ttctagcacg acggctgggt ggcgacatta
     3241 ccgtggtgag cgaagtgggt ttggggagca acttccgatt ggtcatacct ttgggtcttg
     3301 ccgatcagta cgagtggatc acctgcaccg cggatgacct cgatgttacc caaaaatcca
     3361 ttcgtgaagt ccaagccgta gctagcgcgg aaaaaggtgc gcccctcacc gccagggttc
     3421 tgttggtcga agattgtcca gatacccaaa gactgtttac ccacattttg tcccaggccg
     3481 gtgctgatgt gaccagtgcc tcaacggggc gagatacgat ccgcttagcc gttcaagcca
     3541 tggtggaaca gcggcctttt gactgtctcc tcctcgacat gcagttgccc gacatcgacg
     3601 gttaccacgt cgctaggatg ctccgagact caggatacac tgcacccatc gtcgccctaa
     3661 cggcgaacat gacccccggc gaacgcgagc ggtgcctggc ctccggatgc gacgactttg
     3721 ccaccaagcc aatcaaacgg caagacttgg tgaaccttgt ggccaaatac gcaccccaga
     3781 cgggcggact ggccccgatt ccgggcgaaa tgtcttcgag gccgtctttg acgacggggg
     3841 ccccactgcc gcagcccgcg agcaactgag gaccgaacac gcacggtgcg tcaaaaggta
     3901 ggaagaatca aacagtagga agacacgccg gaccaagggg ttcgaacgtc ttgtacgtca
     3961 aaatgaactc gcggtgacct aaggcctcgg agactgtttg tttcccgccg gcgactgcaa
     4021 tcatgaattg ccatattcgc tcggcgactt cctccagcgt gcttttgccc gtaagaacct
     4081 ccccggcgtt gatgtccatg tcggatttca tgcgggcata tgtttcggga ttggcgcaaa
     4141 tcttgatcac gggtgaaatc gccgagccaa caaccgatcc ccggcccgtg gtgaatagga
     4201 tgagatggca cccgcaggcg attaactcca cgatctcggc gttgtccgcg atattgggga
     4261 acccaaagcg gacctcacca tcgggaacaa catctaaaag atacaaccca ggggacggcg
     4321 gaagatcacc cggcttgagg acaccagtga tcgggcgatg tccgcttttg aggtatgccc
     4381 ccagcgactt ttcctcgatg gttgttaaac cgccctctgt gttgcctggg gcaaaactgg
     4441 catgtcccag ggtagcataa tatcgggctg ccttctctac gcaagcacgt aacacgtcgg
     4501 ccagttccgg tcgagccgcc cgttcggcta agagcgattc acatcccagc aattcgccgg
     4561 tctcttcgaa gatagccgtc gcaccgctat caacaagtcg atcaaagacg agaccacacg
     4621 ccggattggc cgtgagtgaa cttgtggcat ccgagccgcc acaaatggtc cccacgacga
     4681 gatcacgtaa ctccatggga caccgcggta ccttggccaa ctccgggagc acactcttga
     4741 tccattgccg tcccgcctta atcgtccgtg ccgtcccgcc aatttcctga attacaagaa
     4801 gctccacagg ccgtcctgat gccgcgatct ccgcacgaag gcgggcgcga tcgaaacttt
     4861 cgcatccgag tgagaccaat agaaccgccc caacgttggg atgggttgtc acgcggctga
     4921 ggaccttgtg ggcgtattca cttgggtagc atccaggaaa tccaatgagt tccacgccac
     4981 gattagcgaa cggcgaagcg atttccgcag ccacgtggtg cgcgcactcc accagataga
     5041 cgacgaccaa gcaattgcgg atgccttttc gcccatcagc acggagataa ccttcccact
     5101 gagctgccgt gcccgatgtt tgtccctggg acgcctgcaa ctcttgtcca ggagttaccg
     5161 ttgtcattcc gtacctcctc gtttttgcgc gacgacgaca acttgcggat atcctaactg
     5221 cgcgattgct cgccctcccc ggcgattatt gaactgtgga gtccgatcgc tcgatttcgg
     5281 ccgggctcgt cccctgttcc agcttgggct cattgggctc gtttggcgtc ggggatggcg
     5341 tcgggccttc ctcaacgacc ggcgaatctt gcgaaggcaa actgtcggaa gagaacgctt
     5401 tcgagtttgc agaaatctcg ccctcggccg agggtccaga cagcgtttcc acaacagccg
     5461 acgtgcccac tgcggaattc tcgccaactg gctcacttac tggctgcgtg cttacgtcag
     5521 aggccgggct ggggcgttcg ggtgtgggaa ggtagtcgct cgctaaattg tgtgtgtgga
     5581 cccattcgcc gggttcaata ttccgcgtgg catgtccgat cggttgacca aacttgatga
     5641 tcttttcgcc ctcggcgata ggacgagtgg ccaccttgcc cgccatcggt acgttggaaa
     5701 tgatctggat cttcacacgg tccagcttca aaacggtctc cgccttaatc ggggcggtgg
     5761 caattgcgat gttatctcgc ggatgtaacc gcaaaagttt tggcttcata tcgccgatcc
     5821 tcaactggcc aaaaatcctc aggtgacagc tccgtgaccc tttccgcagg cagatccctg
     5881 ttgaaaaatc tcccctcaga gagggccatt tcctgccaaa aaagatggcc gacgttcaaa
     5941 tacataacct accacggcag atgggcgaag accagaggcg aatcgctatc gccgggttct
     6001 gcggcggagt tgctcacccg taaccgtatt gttccaactc ttcttccgtc atgccgtagt
     6061 aatgcccata ttcgtggaga atcgtgatcc gtatctgctt tttgagacgc ttctcgtcca
     6121 cataaccgtc acgaccgacg gcggaccaca gtagcccctc ccggtaaatg gtcacgcgat
     6181 ctggtaacgt aaccgaaggt gcgtcgaagt gcttctcgtt gagcggtacc ccggtgaata
     6241 atccccacag ttctcgccga ttgcgtatcc ccgtttggcg catcaccctt cgtgaaggat
     6301 aatcttcaac atagaggggg actttgtcca ttgcgtctcg gacgagctgt ggcaactcct
     6361 cgatgacttc ctcgaggatg cggtcgaagt acctccgaga gtgtttatcc atagcccgag
     6421 ccaagtaatt caaccgatgt ggttttactg ctttttcccg tgttcgccgc tgcaagtgtc
     6481 ctcgtgatca tcaaatgcca gtaattcaac acagacccac gaccaggatc acgtgacttg
     6541 catcaccgcg cgtgattggc ccataactga tgatcgaatt cccacccttt attttcgcaa
     6601 cctgtcaatc gggagccgag aatgtgctca agagcgaggt tgcgcgtaat tatccgtttc
     6661 ttcggcctgc cttctcgcgg cgaggttttg ttacgttcaa gttcgctgag ggcgtcaagc
     6721 cggacgaagg tttttcttta gaggccgttt tcgcacggac atggggattt tgctttggcc
     6781 gtgttccggc gtccgattgg caagtcgcgg cgaacgaagt gtgggcaaaa gttgccaaca
     6841 ttcccgtcgg ctggattcat gtttggcctc gggacacttc cccgccagga cggagcgaac
     6901 ccgaaaagcc atggccagcc gagatcagcc aaattcaaca ggtcttaatg gagcacgcgc
     6961 cgaccaaggc gattcccccc aatgtgtcgc aggaaagtac cccatgcccg ccgggcgttt
     7021 gggtgcttga ttgtgtggtg gtggaaccaa ccgagtggtg gattgggatt caccgggccg
     7081 ggagcttgga atcccgttat cctgggggca ggttcccgat tgttttacca gaatacgccg
     7141 tttcgcgggc atatttgaag attgaggaag cgttgcggtg gagtgggttc aagattcccg
     7201 ccaatgcccg ctgggcagaa ttgggcagcg cccctggcgg ggcgagccag gcgctgctcg
     7261 atcgaggcta cgaagttttg ggggtagatc ctgcggaaat gcatccgctt gtgttatccc
     7321 accccaaatt tacccatctg cggcgacgga cgccccaagc tccccgccgg gcgtttcgaa
     7381 aggtccgctg gctggccgcc gacatcaatg ccgcgcccaa ctacactttg gatgccgtcg
     7441 agagcattgt gacttatccc caggtcaaca tccgcgggat gatccttaca ttgaagttgc
     7501 cgaactggca gctcgccgaa aacattcccg agtacatgga gaggattcgc agctggggtt
     7561 ataacatcgt aaaggcccgt cacttgtgga ataatcgaca ggaagtctgc gtggcggccc
     7621 tgatgaagcc gtttcgtcgg aagccctccc actcgtgacc cgcccggaag ttttgcacaa
     7681 tcgttaattc tttgtgatct gggaaggcca cgtttgattt ggaggaccgt gccgttgtgg
     7741 gcatgcacct tcatgcaaaa aagaagtgag caatacattg cttggctttg cctgtatccg
     7801 ataagcataa ggacaccttc gtgacgcgtc gtcatcgagc acagtcaaag tgtttactga
     7861 ttgagatgtc acggagggtt tttgcaagcc gcgataggaa ttcaaccaag cctctagcct
     7921 tcttcggagg aaaacgatgg agtttgcaac cccctgtttt catgtgtcat tttatgttgc
     7981 gatcctcgtt ggttggcttt tgccttcgat tggagcgggc ttagaaccgg ggctgtaccc
     8041 ttcgacgcgg gactatccat ttacccgagc tatggctcct gattatccca atttgcctag
     8101 tctgccaacg ccgggcgaca actcccgctc ggcgactgtg acctcctcgc ggcttgaaca
     8161 ggccggggac gatgcagagg atgcgattat agaagggccg gacatggcgc tagaatcggc
     8221 tgattcacaa tcggccggcg cgatggtccg agaggaggga accaaaccgg acgatgaggg
     8281 tcttgagaaa caggacgtca tggaggcacc tcaatcgccc gatgggccga gtgctgctcc
     8341 ccagaaacca aatgctgatg gtgattcatc cacagacaag ccgtcagccc gaagatcgac
     8401 tattgaccca acctcggtag gacgtccgct agccgcggga atcgccaaac tggtggctcc
     8461 cgagatcgag tctcaaatgg aaggcgcaaa cattacggcc cgatttcagc agtggcgctc
     8521 ttatgcggcc tcgcgtttgg cggcaacgcg ggctgactac acaagctccg aaatcaatgg
     8581 catggcacga ttgaagtggt acgagcacct cttgctcaat ccgttgaatg tgcctcatga
     8641 aacagaggaa tttacgcgac tgcttcacgc cggttttacg gcc
//
LOCUS       DAOPTR010000018         8669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_6776_length_8669_cov_4.193754, whole genome shotgun sequence.
ACCESSION   DAOPTR010000018 DAOPTR010000000
VERSION     DAOPTR010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 8669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8669
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6776_length_8669_cov_4.193754"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="PKI05_00725"
     CDS             complement(<1..1027)
                     /locus_tag="PKI05_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50745.1"
                     /translation="MFRYRQSIHSQSQLRHAAGHLRGAVAAHNAEAKRLTLQEFRKRQ
                     AEYWFPILEAISVALAVSRSRFVRGGEFHDPVSEAIETAQPDSGFSSFVEPLPQAPLQ
                     TIDPLEDFTTYTEQDPNNRLTVSTYAVTASNLTIGESAWLVKDYGNAAFGEQFQHKFR
                     FRFDKVGFVPVYADVWAVSNVVADGYTWSNNRQQALNAFLYQDSTATRIYLRDSEDGS
                     NDQGYVDIALATDYYASVVRNGTACTLYVYSDANRTQLVGVTSVTLAQGRVYRYVFAA
                     HSHNVGITGRTISFTVGDFDLGFPEPSSSAPSSSVSASSSFVSVSASSVSASSSFVSV
                     SASSVSAS"
     gene            complement(1029..1277)
                     /locus_tag="PKI05_00730"
     CDS             complement(1029..1277)
                     /locus_tag="PKI05_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50746.1"
                     /translation="MKDTVVEQLTSIEKQIGSLEGRIVMLEAQLRNIHQELREMRSVL
                     YGNGKPGLVSKVQVVWLVLMYASGLVTAIAAQFASRVL"
     gene            complement(1284..1889)
                     /locus_tag="PKI05_00735"
     CDS             complement(1284..1889)
                     /locus_tag="PKI05_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50747.1"
                     /translation="MSITTTRSGDAKNFRGRALKLVSNGSEQTTLLVPVQLSRGVYAL
                     NLWAIASASLSSGQFQIRLLDGVNGNTFTDTAGNSCLLTVPCNQLSTSLQKAFTTIWS
                     ISRDFLGYLELKVTTPWTSGQAVFLDEIAMAPARELYQGGLWFAAFRNALLGAVTNKW
                     TITVSNNRAGKVLEYTHRLFGLTDITLPRTGSTLIPESVLG"
     gene            complement(1908..2879)
                     /locus_tag="PKI05_00740"
     CDS             complement(1908..2879)
                     /locus_tag="PKI05_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50748.1"
                     /translation="MALKLYDAANQDGLFNLLGKAYYVWEQLAAAYGANVPTAVNNYL
                     TAVERLTDNLRYVDAMEGIDLDSWRNSEATLTRSLLSALERLAVDFTQTEYPGVLTAS
                     EAVTCLVQEMQRQGYYVAQPSVSASITADASNTNPQPALVCGVRDVNGYIRPTIVPES
                     LYVYHNQDASQLVLASAESAPWESALWPRGSGIQGLYSLLDYRDSLVPNAGFEDVSGG
                     TPSQWLVIAGSSSTIQISEVEQQQVTISGSPSSGWYALKYTAADGKTYTTTPLAYNAS
                     GSTLQSALRQIPELAAVTVGTTGTEPNFTHTITFEGVPGNPSQLVAN"
     gene            complement(2869..4905)
                     /locus_tag="PKI05_00745"
     CDS             complement(2869..4905)
                     /locus_tag="PKI05_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50749.1"
                     /translation="MASVTVLRYGNIFLLNCHTRVHEVTVKYDPSDTDRMFLNLRLVV
                     AGYLVPGVEPMSYSPYQFLDQADKGGILLMGQPVDRSDLPLLLAELNKPRRWLEYFVV
                     ERNRDQQNQIQTNIYPIWAVMPMTEYPNVAAFSTQRRDAIVLEVANGPKPRGVRVEQY
                     FPATGLAKVEFTVEASLMAVPFGKSFADLETERADAALHRWGGEINNRWTIEESMDEN
                     FYVTRRIAGRLQVAGAACVDHILKNQPGQIPPQLRRYLAFPLLERSFIRQSIMYRSSE
                     DGLTCDYEIVDVQVAECPPWPATSMTGKVDLMIDEAFTCRLGANFQLGGPPHVNRKLL
                     IQLAFKIIESVTLGSWQKLNDAKANCYLDSARITTYYGQRQTVDVAVTIRLQPAGLDP
                     DGKQQQPGDWITNFQKTYIGRPIDFTMPFGKRRQVRYDKGAGALPSLYGFTHQGERSP
                     AALLLLACYLQEPQSHHDMPFVKTSKPRVEKIRLPQAQDPEETTETPGTEEAEIEEAE
                     IEESKNTAILVTPETLYTFAALRSTYTTDSYQVGLPRSVPDEDGRTTLFTKLAEPACY
                     RVIEFETERVGDWPKIPASPESYPITEKLQAHLCWKQVTPHAPTPSKDGRQLVYKVTG
                     TYVYQLEAPPPSYDVGLLPVTNPAHLLKDGRATARFEEDATLTGFEKDDMRHGP"
     gene            complement(4913..6214)
                     /locus_tag="PKI05_00750"
     CDS             complement(4913..6214)
                     /locus_tag="PKI05_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50750.1"
                     /translation="MAPAVSALMALLRSAMAALPRMLQAAGQNLSRVANLAVPGSKAR
                     AGTVQVLPMRKASPVQTLTVRSPRPKAARPEQGPEKARKKQPQPERQEIRPRAAPRPP
                     RADVIETAAEEENARELAVRLEALRQGQAPVAPPGPALEQASAPVGAPGTTPPTATAP
                     AGPETPSPLAPQPAKPTQGLPGAEQARRPQGDARPSASTVEAERKAKEAQDKLDQNTD
                     ATQENTEALKQQEKAQRELAQVGNRFGRFLTSAIMSGPMVGPALSNLSRMVEGIAQGV
                     LGRYAEVAKFAPEAQWSLLRLQHQQTLLSAQSVRALSGNINQLAKVLADTQRAWAPFR
                     NVMAAGIMSALTIYLRGLESIGKLMTNVLHGLNRILQAISFGALNLDDLLNDRGNQMN
                     SIASEFFGRLSRSDWHGLTPPPRPMPRFERPFGPLQPPPRR"
     gene            complement(6214..6357)
                     /locus_tag="PKI05_00755"
     CDS             complement(6214..6357)
                     /locus_tag="PKI05_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50751.1"
                     /translation="MLWLSILSLQRQEEQNPNGHPFRWETQLVTVPDSVNLDTFDWNM
                     GVF"
     gene            complement(6417..6764)
                     /locus_tag="PKI05_00760"
     CDS             complement(6417..6764)
                     /locus_tag="PKI05_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50752.1"
                     /translation="MIDWFRSWTYRTYARRGFLVFRLGSQWYVRDPIRILFRLNQEKV
                     SEVLQTVQAQSNLGNYDPSQIDHAYQLIAEAFEIPIGPEGASYQELGELMLVFQEWFE
                     AQKKSTNTSSPSP"
     gene            complement(6761..7294)
                     /locus_tag="PKI05_00765"
     CDS             complement(6761..7294)
                     /locus_tag="PKI05_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50753.1"
                     /translation="MAVLPVVRGLAEVYVNTGTNDTYELLGLTRNGVTFTRQAFQMPV
                     PCDSHGGDEGTPLDMIFLDMIVRIDCELTSFDDEVRKKIVAMSKRKTGEQYNTLPVPG
                     TLLFQSGRYFGVKISSNIADRSDGKVLAFTFPRCLITTDPMTDNWGRRFTTLHFLATA
                     YRDPTANLLWTEELLTP"
     gene            complement(7304..7828)
                     /locus_tag="PKI05_00770"
     CDS             complement(7304..7828)
                     /locus_tag="PKI05_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50754.1"
                     /translation="MAEMGYATPADLVTFFNEVLVEDLASDSGVPEDAAASERIQACL
                     DAASGRVEAALLTSGRYRVEDLQGLSGNSGWYLKWLVCAIALGYLVFNRIARLDEGTK
                     AIIEQAEKQLELLSKGVNILNLPEKVQATEIAVQGPILAERIALNVLPLRTRHFYPSE
                     AQRLPLDRTYPEES"
     gene            complement(7821..8177)
                     /locus_tag="PKI05_00775"
     CDS             complement(7821..8177)
                     /locus_tag="PKI05_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50755.1"
                     /translation="MMPLNTKGLADFLKIIAVLREIGIDTIDVLIKGPNVQDEEAFRA
                     WVRATVKVGKTLAVVTPNVYDDAVMNVLAALVESDPAWNAFYGMVRALLNGQPVAKDS
                     PAAVVAHDLVKVIQDG"
     gene            complement(8181..>8669)
                     /locus_tag="PKI05_00780"
     CDS             complement(8181..>8669)
                     /locus_tag="PKI05_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50756.1"
                     /translation="QAAGLPFVHLSPASLYKQVNGGVDAGSSIDDNLQAISRNGVLPV
                     SLWGDEISWRKSFPQDWKAEAAKYRLTEWYDIGTWEEFVTAVLLGFPVCYGVWWGGGG
                     HAICAVSVVYENSQPAIRFLNSWGTDWGDAGFGTMSKKQVTTGIGYFGAWAARVPVYS
                     GG"
BASE COUNT         1706 a         2523 c         2680 g         1760 t
ORIGIN      
        1 ttgacgccga aacagagcta gccgataccg aaacgaaact gcttgacgcc gaaacagagc
       61 tagccgatac cgaaacgaaa ctgcttgacg ccgaaacgga gctactcggc gcagaagagc
      121 taggttctgg aaagccaagg tcaaaatcac cgacggtgaa actgattgtt cgtccggtga
      181 tccccacgtt gtggctgtgc gcagcgaaca catatcgata aacccttccc tgggccagag
      241 tgaccgaggt tacaccaacc aattgtgtgc ggttggcgtc actatagacg tagagcgtgc
      301 acgcggtgcc gttgcgcacc acgcttgcgt aatagtcggt ggctagggcg atatcaacgt
      361 aaccctggtc atttgagcca tcttcgctat cccgaaggta gattcgcgtt gcggtactgt
      421 cttgatacag gaacgcattg agcgcttgtt gacgattgtt ggaccacgta tagccgtcgg
      481 caacaacgtt actaacggcc caaacatcgg cataaaccgg aacaaagccc accttgtcga
      541 agcgaaagcg aaacttatgc tgaaattgtt cgccaaaagc cgcgttaccg taatctttca
      601 ccaaccacgc cgattcaccg atggtcaggt tggaagccgt tacggcataa gtactaacag
      661 taagtcggtt attggggtct tgctctgtgt aggttgtaaa atcctcaagc gggtcgatgg
      721 tctgaagcgg ggcttgtggc agaggttcga cgaaactgct aaatcctgaa tccggctgag
      781 ccgtttcaat cgcctccgat actgggtcat ggaattctcc gcctcggaca aaacggcttc
      841 tgcttacagc taacgcaaca ctgatggctt ccaaaatggg aaaccaatat tccgcttgcc
      901 gcttacgaaa ctcttgcaga gtcaaacgct tggcctcagc attgtgcgca gcaactgccc
      961 cgcgcaaatg acctgctgca tgacgcaatt gactttggga atggatcgac tgccgatatc
     1021 gaaacatttc agagcaccct tgatgcaaat tgggcggcga tcgccgttac taacccgctt
     1081 gcatacatga gaacaagcca cacaacttgg actttgctca cgagtcccgg cttgccattg
     1141 ccgtagagaa cactccgcat ttcacgcaac tcctgatgga tgttgcgcaa ctgtgcttct
     1201 aacatgacaa tgcggccttc caaactgccg atttgctttt cgattgatgt gagttgctca
     1261 acgacggtat ccttcatact tcctcaccca aggactgatt ctggaattag cgttgaacct
     1321 gttcgcggta acgtaatatc cgtcaatccg aacaaacgat gggtatactc caaaaccttt
     1381 ccggctcggt tattgcttac ggtgatcgtc cacttgttgg tgaccgcccc caggagggcg
     1441 ttgcggaagg ccgcaaacca caggcccccc tgatagagtt cccgggcggg ggccatggca
     1501 atttcatcca gaaaaacggc ctgaccgctg gtccaggggg tggtcacttt gagttccagg
     1561 tagccgagga agtcccgcga gatggaccag atcgtagtga aggccttttg gagcgaggtg
     1621 gaaagctgat tacaagggac ggtcaagagg caactattac cagcggtatc ggtgaaagtg
     1681 ttgccgttga ccccgtccag gagtcggatt tggaactggc ccgacgacag actggccgag
     1741 gcgatggccc agaggttgag ggcgtacacg cctcggctca gctgaacggg gactaaaagg
     1801 gtcgtctgtt ctgaaccgtt ggagaccagt ttgagggccc gtccgcggaa gtttttggcg
     1861 tcgccgctgc gggtggtcgt gatgctcacg gaaccggtgt tgaagctcta attggccacc
     1921 aactggctgg gattacccgg aaccccttca aaggtgatgg tatgcgtaaa attgggttca
     1981 gtcccggtgg tgccgacggt aacggccgca agttcgggga tttggcggag ggcgctttgc
     2041 agggtgcttc cggaggcgtt gtaggccagg ggcgttgtcg tgtaggtctt accgtcggcg
     2101 gcggtgtatt tgagggcgta ccagccgctg ctgggcgacc cgctgatcgt gacctgctgc
     2161 tgctcgactt ccgatatttg aatggtgctg ctggaaccgg cgatgaccag ccactgcgat
     2221 ggggtgcctc cgctcacgtc ttcaaaaccg gcgttgggaa cgagcgaatc gcggtagtcg
     2281 aggagtgagt aaagaccttg aattcccgaa cctcgcggcc agagggcgga ttcccagggg
     2341 gccgactctg ccgaggccag caccaactgg gaggcgtcct ggttgtgata gacataaaga
     2401 ctctcgggaa cgatggtggg gcggatgtag ccgttgacgt cgcggacgcc gcagactaaa
     2461 gcgggctgcg ggttcgtgtt cgaggcatcg gccgtaatgg aagcggaaac gcttggttgg
     2521 gccacgtaat agccctgccg ctgcatctcc tggacgaggc aggtcacggc ctcagaggct
     2581 gtgaggacgc ccggatactc ggtctgggtg aaatcgaccg ccaggcgttc gagagccgaa
     2641 aggagagagc gcgtcagggt cgcttcgctg ttcctccagg agtccaggtc gatgccctcc
     2701 atggcatcga cgtagcggag attatccgtc aaccgctcga cggcggtgag gtagttgttg
     2761 acggctgtgg ggacattggc cccgtaggcg gcggccagct gttcccagac gtaataggcc
     2821 ttacccagga ggttaaacag tccatcctga ttggcggcgt catagagttt aagggccatg
     2881 cctcatatca tccttctcaa aacctgtgag cgttgcatcc tcttcgaacc gggccgttgc
     2941 ccgtccgtcc ttcagcagat gggccggatt cgtcacgggg agcaggccca cgtcatagct
     3001 ggggggcggg gcctccagct gatagacgta ggttcccgtg actttataga ccaactggcg
     3061 tccatccttg ctgggcgtgg gggcatgggg ggtgacctgt ttccagcaga gatgggcctg
     3121 gagtttctcg gtgatggggt aagactcggg gctggccggg attttcggcc aatcgccgac
     3181 ccgttccgtc tcgaattcga tgacgcgata acacgcgggt tccgccagct tggtgaagag
     3241 ggtcgttctc ccgtcctcgt ccgggacgct ccgcggcaag ccgacctggt aggaatcggt
     3301 ggtgtatgtc gagcgaaggg ccgcaaaggt gtacagggtc tcgggcgtta cgaggatggc
     3361 cgtgttcttc gattcttcga tctcggcctc ttcgatctcg gcctcttcgg tcccgggggt
     3421 ctcggtggtc tcttcggggt cctgggcctg cgggaggcgg attttttcca cccgtggttt
     3481 ggaggtcttc acaaacggca tgtcatggtg actctgcggc tcctggagat agcaggccaa
     3541 aaggaggagg gccgccggag agcgttcccc ctgatgggtg aacccgtaca ggctggggag
     3601 cgcccccgcc cctttgtcat agcggacctg tcgccgcttg ccgaagggca tcgtgaaatc
     3661 gatgggacgg ccgatgtagg tcttctgaaa attggtgatc cagtcccccg gctgctgctg
     3721 tttcccatca ggatcgaggc ccgctggctg gaggcgaatt gtgactgcca catcgaccgt
     3781 ttgacgctgg ccgtaatagg tggtgatccg ggcgctgtcg aggtagcaat tggccttggc
     3841 gtcgttgagt ttctgccagg agccgagggt caccgattcg atgatcttga aggccaactg
     3901 gatgaggagt ttgcggttga catggggcgg cccgcccagt tgaaaattgg cccccaggcg
     3961 gcaggtgaag gcctcgtcga tcatgagatc gaccttgccg gtcatggacg tggcgggcca
     4021 gggcgggcac tccgcgacct ggacatcgac gatctcataa tcgcacgtca aaccgtcttc
     4081 gctggagcga tacatgatcg actggcggat gaaactccgc tccaggaggg ggaaggccag
     4141 atagcgtcgc aattggggag gaatctgacc gggttgattc ttgaggatgt gatcgacgca
     4201 ggcggccccg gcgacctgga ggcgtccggc gatccggcgg gtcacataga agttttcatc
     4261 catggactcc tcgatggtcc agcgattgtt gatctcgccg ccccagcgat ggagggccgc
     4321 gtcggcccgt tcggtctcca ggtcggcaaa ggacttgccg aaggggacgg ccatcaggga
     4381 tgcctccacg gtgaactcga ccttggccaa cccggtggcc ggaaaatact gttccaccct
     4441 gaccccccgc ggcttaggcc cgttggccac ctccaaaacg atggcgtccc gtctctgggt
     4501 gctgaaggcc gccacattgg gatactccgt catcggcatg acggcccaga tggggtagat
     4561 gttggtctga atctggttct gctggtcccg gttccgctca acgacaaagt actccagcca
     4621 gcggcggggt ttattgagtt ccgccaacag gagggggagg tcagaacggt ccacaggctg
     4681 ccccatgagg aggatcccgc ctttgtcggc ctgatccagg aattgataag gagagtaact
     4741 catcggctcg acgccgggga ccaggtagcc cgccaccacc aggcggagat tgaggaacat
     4801 ccgatccgtg tcactcgggt catacttcac cgtcacctca tggacccgtg tgtgacagtt
     4861 gaggaggaag atgttgccgt aacgaaggac cgtgacgctg gccatggatc gatcaccgtc
     4921 tgggcggcgg ctggagagga ccaaagggac gttcgaaccg cggcatggga cgcggcggcg
     4981 gcgtcaggcc atgccaatca gaccgcgaaa gccgaccgaa aaattctgac gctatggaat
     5041 tcatctgatt cccccgatca ttcaggagat catcgagatt caacgccccg aacgatatgg
     5101 cctggaggat cctgttcagc ccatgcaaga cgttggtcat gagcttgccg atggattcca
     5161 gcccccgcaa atagatcgtg agggccgaca tgatcccggc ggccatgacg ttgcggaagg
     5221 gggcccaggc ccgctgggtg tcggccagga ccttggcaag ctggttgata tttccggaga
     5281 gtgcccgcac cgattgggcg gaaaggagcg tctgctgatg ctggagccgg aggagggacc
     5341 attgggcctc cggggcgaac ttggcgactt cggcatagcg acccaggacg ccctgggcga
     5401 tcccttcgac catccgcgac aggttggaga gggccgggcc taccatgggg cccgacatga
     5461 tggccgacgt gagaaaacgg ccaaaacggt tgcccacctg ggccaattcc cgctgggcct
     5521 tttcctgctg tttgagggcc tccgtattct cctgggtggc gtcggtgttc tggtcgagct
     5581 tgtcttgagc ctctttggcc ttgcgttcgg cctcgacggt ggaggcgctg gggcgggcgt
     5641 cgccctgagg acgtcttgcc tgttccgctc ctggaagccc ttgcgtcggc ttggccggct
     5701 gcggggccaa tggggatggc gtttccggac ccgccggggc cgttgctgtg gggggcgttg
     5761 tgcccggagc acccaccggg gccgaggcct gttcgagggc cgggccgggc ggcgctacag
     5821 gggcttgacc ctgccggagg gcctccagcc ggacggccag ttcgcgtgcg ttttcctctt
     5881 ccgccgccgt ctcgatgacg tcggccctcg gcggcctggg agccgcacgt ggcctgatct
     5941 cttgtcgttc aggctgcggt tgtttcttgc gtgctttttc ggggccttgc tcaggacgcg
     6001 ccgctttggg acggggcgac cgaaccgtca gcgtctgcac cggggacgcc ttccgcatgg
     6061 ggaggacctg gacagtcccc gccctggcct tgctacctgg cacggccaga ttggcaaccc
     6121 gggaaaggtt ctgccccgcc gcctggagca tccgcggcag ggccgccatg gccgaacgga
     6181 gcaatgccat aagtgccgag actgcgggag ccatcaaaac acccccatgt tccagtcaaa
     6241 ggtatccagg ttcacgctat ccgggaccgt caccaactgc gtttcccagc gaaagggatg
     6301 accattcggg ttttgctcct cctgtcgctg caggctgaga atcgaaagcc ataacatcgc
     6361 ccagaatccc cgatcccgcg gatcgaccag caggggtatg gcctggattc ccagactcag
     6421 ggcgaggggg atgatgtgtt cgtacttttt ttttgcgcct caaaccactc ctggaagacg
     6481 agcatcagtt ccccgagttc ctggtaggag gccccctccg gtccgatggg aatctcaaag
     6541 gcctcggcaa tcagctgata ggcgtggtcg atctgggacg gatcgtagtt acccaggttc
     6601 gattgggcct ggacggtctg gaggacctca ctgaccttct cctggttgag gcgaaagagg
     6661 atgcggatgg ggtcccgcac gtaccattgc gatcccaacc gaaacaccaa aaacccccgt
     6721 cgggcgtatg tccggtatgt ccaggagcgg aaccagtcga tcatggcgtc aggagttcct
     6781 ccgtccagag gagattggcc gtcgggtcgc gataggccgt ggccagaaag tggagcgtcg
     6841 tgaagcggcg gccccagttg tcggtcatgg gatcggtcgt gatgagacac cggggaaagg
     6901 tgaatgccag gaccttccca tccgaccggt cggcgatgtt gctgctgatc ttgaccccaa
     6961 agtaccgccc gctctgaaac aggagggtcc ccgggacggg caaggtgttg tactgttcgc
     7021 ccgtcttccg tttgctcatg gccacgatct ttttccgcac ctcatcatcg aagctcgtca
     7081 gttcacaatc gatccggacg atcatgtcca gaaagatcat gtcgaggggg gttccctcat
     7141 cgccgccatg cgaatcacac gggacgggca tctgaaaggc ctgccgggtg aacgtcacgc
     7201 cgttccgcgt caggcccaaa agctcgtacg tatcgttggt cccggtgttc acgtaaactt
     7261 cggccagacc gcgaacaaca ggaagcactg ccatacccac acctcacgat tcttccggat
     7321 aggtccggtc caggggcaac cgctgggcct ccgagggata aaaatgtcgc gtccgcaggg
     7381 gcaagacgtt gagggcgatc cgttctgcca ggatcggccc ctgaacggca atctcggtgg
     7441 cctggacctt ctccggcagg ttgaggatat tcacaccctt gcttaacagt tccagctgtt
     7501 tttccgcctg ttcgatgatg gccttggtcc cttcgtccag tctggcaatc cgattgaaca
     7561 cgagatagcc aagggcaatg gcgcagacca gccacttgag ataccagccg ctgttgccgc
     7621 tcagcccctg gaggtcctcc acacgatacc ggccgctggt cagtaaggcc gcttcgacac
     7681 gcccactggc ggcatctaaa caggcctgaa tccgctcgct ggcggcggcg tcctcgggca
     7741 ccccggagtc cgaggccagg tcctccacca ggacctcgtt gaaaaacgtc accagatcag
     7801 ccggtgttgc gtagcccatc tcagccatcc tggatgacct tcacaagatc atgggccacc
     7861 acggcggccg gtgaatcctt tgccaccggc tggccgttga gcaaggcccg caccatcccg
     7921 taaaaggcgt tccaggcagg atcgctctcg accagggccg ccagcacgtt catcacggcg
     7981 tcgtcgtaaa cgttcggcgt cacaacggcc agggtcttac caaccttgac cgtggcccgc
     8041 acccacgccc gaaaggcctc ctcatcctgg acattggggc ctttgatcaa gacatcaatc
     8101 gtgtcgatcc cgatctccct caaaacggca atgatcttga ggaaatccgc cagacccttg
     8161 gtattcaacg gcatcatcgt tcaacctccc gaatagacgg gaacacgggc cgcccaggcc
     8221 ccaaaatagc cgatccccgt cgtgacctgc ttcttgctca tggttccgaa tccggcgtcg
     8281 ccccaatccg tcccccagga attgagaaac cgaatcgccg gttgactgtt ctcgtacacc
     8341 acggacacgg cacaaatggc gtgtcccccg ccgccccacc agacgccgta acacaccgga
     8401 aaccccagca ggaccgccgt cacgaattct tcccacgtcc cgatgtcata ccattccgtg
     8461 agccggtact tggcggcctc ggccttccag tcctggggaa acgatttccg ccaactgatc
     8521 tcatcgcccc agagactcac cggaaggacc ccattgcggc tgatcgcctg caggttgtcg
     8581 tcaatggagg acccggcatc gacgcccccg ttgacctgct tgtagagact ggccgggctg
     8641 aggtggacaa agggcagccc cgccgcctg
//
LOCUS       DAOPTR010000019         8541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_6898_length_8541_cov_3.493754, whole genome shotgun sequence.
ACCESSION   DAOPTR010000019 DAOPTR010000000
VERSION     DAOPTR010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 8541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8541
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6898_length_8541_cov_3.493754"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PKI05_00785"
     CDS             complement(<1..237)
                     /locus_tag="PKI05_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50757.1"
                     /translation="MFVTPDYRRWLSTSPQGVETEAIAIQTFYDNHNDDSAFFKPMLT
                     RSQQRLLNPTLKWPIFPVPQLGLTSARPSASGNST"
     gene            236..1195
                     /gene="cysK"
                     /locus_tag="PKI05_00790"
     CDS             236..1195
                     /gene="cysK"
                     /locus_tag="PKI05_00790"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOA50758.1"
                     /translation="MSIPSQVRCYDEITQCIGNTPLVRVRRLAAGYGATLLAKLESFN
                     PLWSVKDRIARAMIDAAERQGLIKPDTVIIEPTSGNTGIGLAYVCASRGYKLMVTMPE
                     SMSLERRRLLKAFGVELVLTPAAEGMPGAVRKAEEIVRENPNFYMPQQFKNPANPEIH
                     RRTTAEEIWRDTGGQVDIFVAGVGTGGTITGVGEVLKSRKPDVRIVAVEPANSPVLTQ
                     KLSGQPIKPGKHGIQGIGAGFIPDVLNLSVIDEVICVEDEAAMETSRQLARFEGFMCG
                     ISCGAAMWAALELAKRPENSGKTIVVVLPDLGERYLSTRLFPE"
     gene            2147..2500
                     /locus_tag="PKI05_00795"
     CDS             2147..2500
                     /locus_tag="PKI05_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOA50759.1"
                     /translation="MNEQLTNPALDQVPAIYSELADDPDLRDLIQMFVDEMPYRAALY
                     EELFRTGDFEELRRAVHQLKGAAGSYGFTPLTEAATALEQSIRHEAPYEQIRSELNNL
                     LRLCAAARLKPEITA"
     gene            complement(2622..3959)
                     /locus_tag="PKI05_00800"
     CDS             complement(2622..3959)
                     /locus_tag="PKI05_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOA50760.1"
                     /translation="MGFHWSSRVLGSLVVFSLFIVAGSSLWGETGDELWTAAEMRIEK
                     YRKGDVIITVRDKDGRPVPNAKVRLQQTRHCFLFGCNIFLWGRIEDSQLQTAYNERFA
                     ELFNYATAAFYWASYERQRGRPDHDYTRRLAEWCREKGIVVKGHPLAWNTADPIWLPD
                     DPDEILRLQLARIEDCVSRFRGLIDRWDVVNEVSEYDRPEFVTRRAPKMSAMWKKVGQ
                     MEFTRQCFETARKANPDATLIINDYVTSPKFERVIEQLVDQDGKRLYDVIGIQSHMHG
                     GTWPNERIWEICERFSRFQVPIHFTETTILSGQRGWERPRPWVTTPEGEEYQAKEVVR
                     FYTMLFSHPAVEAITWWDFSDLRAWQGAPAGLVRRDMSPKPAYTELYKLIKQKWWSDE
                     TLSTDSQGRATTRAFAGDFVISAQAPGFSEAKESVKVEWRKPNEFVVVVEKEG"
     gene            complement(4013..4702)
                     /locus_tag="PKI05_00805"
     CDS             complement(4013..4702)
                     /locus_tag="PKI05_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOA50761.1"
                     /translation="MEAKNLETVPEAVVFDMDGLMFNTEDIYFAVGEELLRRRGKNFT
                     RELSDAMMGRPPQASFEIMIQWHNLSDTWQELAAESEDLFIERIQGRIAPMPGLLELL
                     TALESANIPKAIATSSSRRTLRAVLACFDLEPRFAFTLTAEDVVQGKPYPEIYLKACE
                     RFGLPPHKVVVLEDSEAGCRAAAAAGTITVAVPGSHSARHDFTCATMVISTLRDPRLY
                     QLLRLPRERNS"
     gene            complement(4786..5154)
                     /locus_tag="PKI05_00810"
     CDS             complement(4786..5154)
                     /locus_tag="PKI05_00810"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOA50762.1"
                     /translation="MPENKSRSPQGEGLQEWPISEHPDGVILSIKAQPGAKKNEVHLE
                     ETGQVKVCVTQQPEKGKANRAVLEVLADFLGVKASQVALVSGKTSQKKQILVYGLSAK
                     EVAEILNGHLAGRNARTRMP"
     gene            complement(5178..5972)
                     /locus_tag="PKI05_00815"
     CDS             complement(5178..5972)
                     /locus_tag="PKI05_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOA50763.1"
                     /translation="MGKGTENRAAFSDLYCINRFVIADCEMSESIEDILERNLNAVRH
                     RIAVAATRCGRSPQEITMVAVTKYVDLSIIQGLLRLGQRILGESRPQQLWQRASELRE
                     YDIEWHLIGPLQKNKVRKTLPIAAWIHSIDSLGLLEAVDRIAGELGVRPKLLLEVNVS
                     GHPTKHGFRPEEMPSVIAQCPQYRHVTICGLMAMAGQEGDLEGARRDFRALRELRDQL
                     QELMGEEVTLKELSMGMSGDFEIAIEEGATIVRVGSALYEGLSLPL"
     gene            complement(5980..7317)
                     /locus_tag="PKI05_00820"
     CDS             complement(5980..7317)
                     /locus_tag="PKI05_00820"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOA50764.1"
                     /translation="MRENRERNDRIPHILDEPMDIRISYGTYGVLEIKSTSLVNVVGF
                     PGSDVAPIEDVNAAVREALDHPLELPALARCVTPGDRVVLALRRGTPRGAEILAAVTR
                     YLLPHLSTEGQLTVLRTESDETAGWGDPRPFLSAEEAQALEVRTHRADRQEELTLLAR
                     FRNGRPLRLNRALIDADVVIPVGCTPPGKAWSAYGTSGAVFPAFSDRMSQLAHWRDVV
                     QKARRGKGPLPEHRDRSGREANWLLGTQFAVEVIPGPCGSVLRIVAGATEAARRTARE
                     EYRRWWCPTVDGKAELVIAGVDHNPSHTPWENLAQAAWTAARLVRRGGQIILVTDWDQ
                     GTIPRLIDENSLAEVSGIWSWSDFLQTHGDLAVPFWLLARVRRYARVCLWTAQSLADE
                     SLSGWRERLSLETTSGEAVINRLVNRVDSCTVFSHASLAWPRVSRGATLRGAG"
     gene            7525..>8541
                     /locus_tag="PKI05_00825"
     CDS             7525..>8541
                     /locus_tag="PKI05_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50765.1"
                     /translation="MRQNERIPSFVVFLCTVCASDPFRLLSIFRSVSTRSVFHLVFIA
                     FLLTLCGCPAKSRESAGPSQAADQKEPLGTVKAGDSPTTEKGQPVQQATGDQAVSQST
                     GGVGTPQAAPPLSTETAEEILQKMVEAYGKARSYGDQGRIVMRGVYNGQPFENRTNYL
                     TAFERPNKLRLIAGEGVLICDGKDLWGFVNFLPGQVLKTNAPDKFTLKTLFTDTVLAN
                     ALMQGPGQVYSLVPPPLVLLLADDPLKTFLYRAKPPEVIPPGTLGNSTCDRIRLARLD
                     GDIILWIDRSTRLLVRVDFPTESIRRSLDGGQLQNLAIYAEFPNAVLDADIDPAAFQF
                     QAPPQ"
BASE COUNT         1970 a         2485 c         2187 g         1899 t
ORIGIN      
        1 ggtagaattg cccgacgcag atggacgtgc gcttgtcaaa cccaattgag gaaccggaaa
       61 aatgggccat tttaaggtgg ggttgagaag gcgctgttga cttcgtgtaa gcatcggctt
      121 gaaaaaagcc gaatcatcat tatgattatc ataaaaggtt tgtatagcta tagcttcagt
      181 ttcgacccct tgtggggaag tcgaaagcca gcgtcggtaa tcaggagtca caaacatgtc
      241 aattccatcc caagtccgtt gttacgatga gatcacccaa tgcatcggga acacaccgct
      301 cgttagagtt agaaggcttg ccgccggcta tggggccact cttttggcaa aactcgaaag
      361 ttttaacccc ctctggagcg tcaaggaccg cattgcccga gcgatgattg acgccgccga
      421 gcgccaggga ttgatcaaac cggacacggt catcatcgag cccaccagtg gaaacacggg
      481 aattggtttg gcgtatgtgt gcgcttcccg cggttacaag ctgatggtca cgatgccgga
      541 gagtatgagt ctggaaaggc ggcggctcct caaggccttc ggagtggaat tggtgctgac
      601 tcccgcggcg gaggggatgc caggggccgt tcgaaaggcc gaagagatcg tccgcgaaaa
      661 ccccaacttc tacatgcccc agcagtttaa gaacccggcc aatcccgaga ttcatcgccg
      721 aacgacagcc gaggagattt ggcgcgacac cggcggccag gtggatattt ttgtggccgg
      781 tgtgggaacg gggggaacga tcacgggggt gggagaagtc ctcaagtccc gcaaacctga
      841 tgttcgcatc gtggcagttg agccggccaa cagtcccgtg ttgacgcaaa agttatccgg
      901 gcagccgatc aagccgggga agcatgggat tcagggaatc ggtgctggct tcatccccga
      961 tgttcttaat ctttcggtca tcgacgaggt catctgcgtc gaggatgaag cggccatgga
     1021 gacttcccgt caattggcca ggttcgaagg ctttatgtgc ggcatcagtt gcggtgccgc
     1081 aatgtgggcc gccctagaat tggccaaacg tccggaaaac agcgggaaaa ccatcgtcgt
     1141 ggtccttcct gacctgggag aacgctacct ctcgacacgt ttgtttcccg agtgattgga
     1201 agtggcacgc tgaggaacca atacggggac aagtttttgg tttttctctt cggacagaag
     1261 aaaagcaaaa gggcggtcac tctttagcga ccgccctttc tcacatcagt ggttacgtct
     1321 cgggtaacga ccgtgttcgc aacgcccagg aggttttaac tcagcgagcc atcagccgca
     1381 cggagcacaa gccggtttct cacaagcggg agcgcagggc tcgcagcaag ctcgcttcgc
     1441 ctcacaacgg gcacgaatcc gggccaagag accaggacgg caagctggct cacatgcggg
     1501 ttcgcaagcc ggttcgcatg caggctgttc acaagcgggc ttctcgcaag cgggggcgca
     1561 tgcgccaccg aggaacaaat cagcccgagc cgtatttagg ccgagcacag tcaccagggc
     1621 cccaagggcc accaagccaa gcagtgtcac gcgcttcatg ttaacactct ccgttcactc
     1681 gaggaactga aacaacacct aacccaattt tcttacccaa acaccttctg gccactattt
     1741 ttcatcgtcc attgcaaagc cccattttac ctaattgtcg ccgttcgccc attcaattgg
     1801 ccagaaaaaa tggagatctg tgcaggatag acagatttta acgaaggaag cagtctcctt
     1861 tcctttgttc tactctcagg tgaaatctct cgcgactaac aaatccaagg ggcgaattat
     1921 ggcgggactg accgcggtgc ttactctcgg accgactggg cgaatcgatc ctacgattcg
     1981 gcgcaacaaa gctgggaaag atttcccaac cacgtcatca ttataagcta gactatctta
     2041 agggatcaag caagcggggt agggtacacc aactatctgt gtcccgcttg ggaaggaggg
     2101 gttttgacac tattagcgat gtggtagcac ggcgaaaggt cgatccatga acgaacaatt
     2161 gacgaatcca gcacttgacc aggttcccgc catatattct gagttagcag acgaccctga
     2221 tcttcgggac ctcatccaaa tgtttgttga cgagatgcca tatagggcag cgctttatga
     2281 agaactcttt cgcacgggcg atttcgaaga actgcgacgg gctgtccatc aactaaaggg
     2341 cgctgccgga agctacggct tcactcccct tacggaggcc gcgactgccc tagagcagtc
     2401 catccgccac gaggccccct acgaacaaat ccgcagcgaa ctcaacaatc tgctccgtct
     2461 atgcgcagcc gctcgcctta agccggaaat aacggcttaa agtaaaacaa ggcgctttcc
     2521 cctcaaaagg aagttattct ccctcgacaa tttaccacac ttggcttacc agcaggccca
     2581 gcgtctttcg tcgcaacact cggcctcaga aatccattac ttcagccttc cttctccaca
     2641 acgacaacga actcgtttgg ttttcgccac tcgactttga cgctttcctt ggcctcggag
     2701 aatccggggg cctgagcgga aataacgaaa tcccccgcga aggcacgagt cgttgcccgc
     2761 ccctggctgt ccgtgctaag ggtttcatca ctccaccact tctgcttgat caacttgtaa
     2821 agctcggtgt aagcgggctt tggcgacata tcccggcgaa caagaccagc tggcgctcct
     2881 tgccatgctc gcaaatctga aaaatcccac caggtgatgg cctccacggc tgggtgcgaa
     2941 aacagcatcg tgtaaaaccg caccacttcc ttggcctgat attcctcacc ttcaggtgtt
     3001 gtcacccaag gtcggggacg ttcccatccc cgctggcctg aaagaatagt cgtctccgtg
     3061 aaatggatag gcacttgaaa ccgcgagaac ctctcacaaa tctcccagat tcgttcgtta
     3121 ggccaagtgc caccatgcat atggctttgg atgccgatta cgtcgtacaa tcgcttgccg
     3181 tcctgatcga cgagttgctc gatgacccgc tcaaacttgg ggcttgtcac ataatcgttg
     3241 atgataagcg tcgcgtcggg atttgccttg cgtgcggtct caaagcactg ccgcgtgaac
     3301 tccatctgcc ccactttctt ccacatggcc gacattttcg gggcgcgccg cgtgacaaac
     3361 tcgggacgat catactccga cacttcattc acgacgtccc aacggtcaat aagaccgcgg
     3421 aaacgtgaga cgcagtcttc aatccgggca agctggagac gcaagatttc atccggatca
     3481 tccgggagcc aaataggatc cgccgtattc caagccaacg ggtgtccctt cacgacaata
     3541 cctttttccc gacaccattc ggccagccgg cgggtgtaat catgatctgg acgaccgcgc
     3601 tgccgttcgt aggaagccca atagaatgcc gcggtcgcat aattgaacaa ctcggcaaaa
     3661 cgttcgttat aggcggtctg aagttgtgag tcttcgatcc ggccccacaa aaagatattg
     3721 caaccaaaca aaaagcaatg ccgtgtctgc tgtaacctta ccttggcatt tggaacggga
     3781 cggccatcct tgtcccgaac cgtgattatg acatcgcctt tgcggtattt ttcaatccgc
     3841 atttccgccg ccgtccatag ttcgtcacct gtttcacccc ataacgacga accagcaaca
     3901 ataaaaaggg agaaaaccac aagacttccg agcacgcgac ttgaccaatg aaaacccatg
     3961 ctttttctcc gacgtctgtt ctgaaatgac cacgctgcgt agcgagtgag ccctagctgt
     4021 ttcgctctcg gggcagcctc aacaactgat acaatcttgg atcccgcaaa gtgctgatca
     4081 ccatcgtcgc acacgtaaaa tcatgtcgtg cgctgtggct accgggaaca gcgacggtta
     4141 ttgtaccggc agccgccgcc gctcggcaac cagcttcgct atcttcgagc acaaccacct
     4201 tgtgaggtgg caatccaaat cgttcgcagg cctttaggta gatttccggg taaggctttc
     4261 cctgaacaac atcctcggcc gtgagggtaa aagcaaatcg gggttccaag tcaaaacagg
     4321 caagtacagc gcgaagcgtc cgtcgtgaac tactggtggc aatggccttc ggaatgttag
     4381 ccgattccaa cgctgttaac agctccagca gtcccggcat tggggcaatc cgcccttgga
     4441 tgcgttcaat gaagaggtcc tctgactccg ccgccagctc ctgccaagta tccgaaagat
     4501 tatgccactg aatcatgatc tcgaagcttg cttgtggcgg gcggcccatc atggcatcac
     4561 ttaattcacg ggtaaagttt ttgccacgac ggcgaaggag ttcctctccc accgcaaagt
     4621 agatatcctc cgtgttgaac attaggccat ccatgtcgaa gacaacagct tcgggtacgg
     4681 tttccagatt tttcgcttcc atcctcgaac tcttcacttt ccttgatttg ctaccgcgtt
     4741 gtgacagtcc aacacaatac tccaccagac cttatggcag ttttactacg gcatacgtgt
     4801 gcgagcgttt cgcccggcca aatgcccatt gagtatctct gccacttctt ttgcggacag
     4861 accataaaca agaatctgct ttttttgcga tgtctttccg gaaacaagtg ccacttgaga
     4921 agccttgacg cccaggaaat cggccaagac ttcgaggacc gcccgattgg ccttgccctt
     4981 ttcgggttgt tgggtcacgc acaccttaac ctggccagtt tcctccaagt ggacctcatt
     5041 ctttttggca cccggctggg ccttgataga aaggataaca ccatcggggt gctccgaaat
     5101 cggccattct tgcagacctt ctccctgggg tgatctcgat ttattttccg gcatactggt
     5161 tcttctcgag agatgaatca caaaggcaaa ctaagcccct catacagagc tgatcccacg
     5221 cgaacaattg tggccccctc ctcgatggca atctcaaaat cgccactcat tcccatggag
     5281 agctccttga gtgtcacttc ctcacccatg agctcttgca actgatcccg cagttcccgc
     5341 agcgcccgaa agtcacgtct ggccccctcc aagtccccct cttggccggc cattgccatg
     5401 aggccgcaaa tcgtaacgtg gcgatattgg ggacattgtg cgatgacgct gggcatctcc
     5461 tccggccgaa atccgtgctt cgtcgggtgg cccgagacgt tgacctctaa cagtagcttt
     5521 ggccggacac ccaattcccc tgcgatccga tcaacggcct ccaacaatcc cagcgaatcg
     5581 atcgaatgga tccatgcggc gatggggagt gttttgcgga ccttattctt ttgaagcggc
     5641 ccgatcaaat gccattcaat atcgtattct ctgagctcac tcgctcgctg ccacaactgc
     5701 tggggacgac tttctcccag gattcgttgg ccgagacgca gcaggccctg gataattgac
     5761 agatcgacgt atttggtgac cgccaccatg gtgatctctt gcggagaacg gccacaccgt
     5821 gtcgcagcga cagctatccg atgtcgcaca gcattaagat ttctttcaag aatatcttcg
     5881 atactctctg acatctcgca gtccgcaatc acaaaccggt taatgcagta aagatcggaa
     5941 aatgccgcgc ggttctccgt gcctttgccc attctcgtat cagcctgcac ctcttaaggt
     6001 tgcgccccga gacactcttg gccatgccaa cgacgcatga gaaaaaaccg tgcacgagtc
     6061 aactcgattg actaaccgat tgatcacagc ctcgcccgag gtagtctcca gcgacaatcg
     6121 ctcccgccac ccgctaagtg actcatccgc gagggattgg gctgtccaga gacacacccg
     6181 ggcatagcgt cgtacccggg caagaagcca aaagggcaca gccaaatcac catgggtctg
     6241 gagaaaatca ctccaactcc aaatgccaga gacctcggcc aagctgttct cgtcgatcaa
     6301 ccgcggaatc gtaccttggt cccaatcggt cacaagaata atttgcccac cgcggcgcac
     6361 aagccgggcg gctgtccaag cggcctgagc caggttttcc cagggtgtgt gtgagggatt
     6421 atgatccaca cccgcaatga ccaactccgc cttgccatca actgtgggac accaccatct
     6481 tctgtattcc tcacgggcgg tccgtcttgc tgcctccgtt gctcccgcca caattctcaa
     6541 gacacttccg cacggtccag ggatgacctc gacagcaaac tgtgttccca aaagccaatt
     6601 ggcctctcgg ccagatcgat cccgatgctc aggcaatggc cccttgcctc gtcgtgcctt
     6661 ttgcacaaca tctcgccagt gggcaagctg gctcatgcgg tccgaaaacg ctggaaagac
     6721 ggcccccgaa gtgccgtaag cactccaggc cttccctggt ggagtacaac caaccgggat
     6781 caccacatcg gcatcaatca acgcacgatt cagccggagt ggccgtccgt tgcgaaatct
     6841 ggcaagcaag gtaagttctt cctgccggtc ggcacgatga gtccgcacct ctaaggcttg
     6901 tgcttcctcc gctgacaaaa acggccgggg gtcgccccat cccgccgttt cgtcggactc
     6961 agttcgcaaa accgtgagtt gtccctccgt tgacaaatgg ggaagcaagt atctagtaac
     7021 cgccgccaaa atttccgccc cacgcggtgt tccgcggcga agcgccagca ccacccgatc
     7081 acccggtgtt acgcatcgtg ccaatgcggg caactctaaa ggatggtcca aggcttcccg
     7141 gacggccgcg ttcacatcct caatgggtgc aacatcagat cccggaaagc cgaccacgtt
     7201 taccagcgag gtgcttttaa tttcaagcac cccgtatgtg ccgtaggaaa tacggatatc
     7261 cataggttcg tccagaatat gcgggattcg atcgttacgt tctctatttt ccctcatccg
     7321 cactgttatt cccagttggg tgtcacacac gggaaggatt tgctaagatg ggcgtaggcg
     7381 cgcttgctcg ggtcgcgcga tgttatttcc agtgcggcct ggtaacggcg atcgttgatc
     7441 gcgttcttcc ctgggcaagg cgggaaacga cctgagcaag tacaaggtgg cgtcgagacc
     7501 aaggattggc aaacggagtt atctatgagg caaaatgaac gaattccctc gtttgttgtg
     7561 tttttatgca cagtttgtgc ctctgatccc tttcggctgc tctcgatatt tcgcagcgtc
     7621 tccacgcggt ccgtttttca tctcgttttt attgctttct tgttgactct ctgcggatgt
     7681 ccggcgaaaa gtagagagtc agctggaccc tcgcaggcgg cggaccaaaa ggagccttta
     7741 ggtacagtta aggcgggtga ctcgccaacg acggagaaag gacagcctgt ccagcaagcc
     7801 actggggacc aagccgtctc tcaatccact ggtggtgtcg gtactccgca agccgctcct
     7861 cctcttagta cagagacggc cgaggaaatc ctccaaaaga tggtggaggc ctacggcaag
     7921 gcccggagct acggagatca gggacgcatc gtgatgcgag gcgtctataa cggtcagcca
     7981 tttgagaacc gcaccaacta cctaacggcg tttgaacggc ccaacaaact tcggttgatc
     8041 gcgggcgaag gagtattgat ctgcgatggg aaagaccttt ggggattcgt caacttctta
     8101 cctggtcagg ttctcaaaac caatgcaccc gataagttca ccctaaaaac cctgtttacg
     8161 gataccgttc tcgcgaacgc cctcatgcaa gggcccggcc aagtgtattc gttagtgccc
     8221 ccgccgctgg tgctgttgct agccgacgat ccactcaaga cgttcttgta tcgggctaag
     8281 cctcccgaag tcatcccgcc tggcaccctt ggcaattcta cgtgcgatcg gattcgcctg
     8341 gcgcggttgg acggggatat catcctttgg atcgacagga gcacccggct cctagtgaga
     8401 gtcgattttc ccaccgaaag catccgacgg tccttggacg ggggacaatt gcagaatctt
     8461 gcgatttatg cggagtttcc aaatgccgtc cttgatgcag atatcgaccc ggcggccttt
     8521 caatttcagg cacctccgca g
//
LOCUS       DAOPTR010000020         8479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_6970_length_8479_cov_3.285613, whole genome shotgun sequence.
ACCESSION   DAOPTR010000020 DAOPTR010000000
VERSION     DAOPTR010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 8479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8479
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6970_length_8479_cov_3.285613"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PKI05_00830"
     CDS             complement(<1..649)
                     /locus_tag="PKI05_00830"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOA50766.1"
                     /translation="MGYRFLALDIDGTLVSTVDAISPSVREAIARAAEAGLEVILATG
                     RRYSRALPFSKILQIETPLVTSSGALTKIPKTHETLFRARFSDEVLRKTLIVLRELGF
                     PAAMLGDTFSHGFDYYIERPNGYRNPYFEDYLEQNSGSERIWDDFLAELPPEIFATFT
                     VGSEAEMKWVESQLHAALPHQLTTNVLRSPRYRGFFCEIMPAGTTKWSAICQLAAQ"
     gene            complement(891..2684)
                     /locus_tag="PKI05_00835"
     CDS             complement(891..2684)
                     /locus_tag="PKI05_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007340693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOA50767.1"
                     /translation="MVNNADLRDKVIRKVLPRVQTPAQYLGGELNSVVKDTSQVCGHL
                     CLCTPDTYAIGMSNHGLQVLYDTMNRRTSWACERVFAPGRDMEAELRHWNLPLYSLET
                     FTPLYRFDVIGFSLQYELSYTNVLTVLDLGRIPLLAEERDLNDPLVIAGGPCAQNPEP
                     MSCFIDLFVIGDGEELLPAVCNAWLEARQTSQSRRDALAKMARQLPHVYVPSLYHAEV
                     QPSTGLMAVCPAEPGIPDVISPAVLADLDGFPLPTAPIVPFVEIVQDRIAMEIMRGCP
                     WRCRFCQSTTIKRPLRYRRVETLVAAAEEAYRNTGTNEIALLSLSSSDYPQFAELITR
                     LREVFAPRHVAISVPSLRVNEQLRVLTQMLTPDRHSGLTLAPEVALDDMRVQIGKRIR
                     NEDLIDGCREAFRSGFDRVKLYFLCGLPGEREADLAGIVELAQEISGLGRSILGRPAT
                     VVANVSNFVPKPHTPYQWHPMQTREYFTQAHQFLRQRCRMRSVQIKCHPVESSLLEGI
                     MTRGDRRMGQAILEAWRRGARFDGWAEQFNPQIWWDVFDDLELPVEETLHRPWSPEAR
                     LPWDHVSIRQGRGYLERERDRAWRDLAALNR"
     gene            2981..4225
                     /locus_tag="PKI05_00840"
     CDS             2981..4225
                     /locus_tag="PKI05_00840"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009101506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOA50768.1"
                     /translation="MKTWFQRLSFVVIVVAFSPVSGRAEQGGRGILPFDRLRQEMVRR
                     DILGGGIKNPRVIAAMASVPRHEFVPPEQRRLAYHDMALPIGYGQTISPPFVVAYMTE
                     QIDPQPTDRVLEIGTGSGYQAAILSGLVAEVYTIEIVEPLARQAEATLRRLGYKNVFV
                     KAGDGYQGWPEHAPFDKIIVTCSPEKVPQPLIDQLKEGGRMVIPVGERYQQTMYLLKK
                     TGGELVKESLRPTLFVPMTGIAESQREVKPDPAKPQLINGDFEEIRPDAPEPPGWYYC
                     KQFELRQNDGAPSGSVYLALRNREPGQPARVLQGFPIDGRQVQEILVTFFVRAQNVRP
                     GRNPQESAGVIITFFGEDRAPVGEATIGPIMGTFPWRHVSRRVQVPAAAREAILGIGM
                     TGAIGELAIDRVEILATSEKKP"
     gene            4530..5315
                     /locus_tag="PKI05_00845"
     CDS             4530..5315
                     /locus_tag="PKI05_00845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03000 domain-containing protein"
                     /protein_id="HOA50769.1"
                     /translation="MRPRILMAFAALGIGVGCLGATSEACWPRHVCGPWYPGPLTALA
                     CWAPCWSVCDVCQPYDPCCYDDGWVLGVRPGPIRRLILGPYRWYRVPFGCCDVCGEVA
                     CTCEPAIVTGPALTPTPAQPFSTPAQPTPAQPSPTPDALPSPSPTPGADIPNQPAPST
                     PLVNPTSYTVPTRNDSGLLTVYVPAEAKVLINGLPTKSTGSRREYVSYGLKEGLQYRY
                     VITAQVEQDGKLYEETREVILTAGEKKGVAFSFQFPTENIAGL"
     gene            5740..6747
                     /locus_tag="PKI05_00850"
     CDS             5740..6747
                     /locus_tag="PKI05_00850"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009094718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOA50770.1"
                     /translation="MFKTLAVVGATGAVGRIMVQLLERSSLTFERIKFLASARSIGKT
                     IEFRGEKYPVQELRPEAFDGVDLAVSSTPDEVAKEFIPEAVRRGCVVVDESAAWRMDP
                     NVPLVIPEVNPHAAFKHNGIIASPNCSTTQMVVALKPLHDYGRVRRVIVSTYQAASGA
                     GVGGTKDLFEGTRAYLENRPYAYTTFAHPLAFNLIPQIGSPRPNGYTSEEMKMVLETR
                     KIMEDESIQICATCVRVPVANSHSESILVETEKKITVEKARELFSKFPGIVVIDNLEA
                     KQYPLPLNCSGRDEVFIGRIREDFSSPNGITFWCVSDNLRKGAATNAVQIVELLLRGR
                     P"
     gene            6774..7610
                     /gene="truA"
                     /locus_tag="PKI05_00855"
     CDS             6774..7610
                     /gene="truA"
                     /locus_tag="PKI05_00855"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOA50771.1"
                     /translation="MRCIRLTLAYDGSNFLGWQRQANGRTVQGVIEAAIEKISGKRSR
                     VLASGRTDAGVHALGQVAAFRTESTLTAETWGKALNAVLPHDIVVLAAEDVPLSFHPI
                     RNAQRKRYCYLLDDGAVRPVIFRQFVWHYPRPLDVDLMRTASEFLIGQHDFAAFETSG
                     APRKTTVRTIFELSVRRVHPYMPSVIAIEIEADGFLYNMVRNIVGSLVEIARGARPVA
                     WLGEVLASRDRRLAGPTAPPQGLFLVSVTYDNLPTFSAANPWFLGSLRMVHAENETRL
                     EQ"
     gene            7746..>8479
                     /locus_tag="PKI05_00860"
     CDS             7746..>8479
                     /locus_tag="PKI05_00860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA50772.1"
                     /translation="MRVLHIITRLIVGGAQENTILTCEDLIHRYHDDVLLVTGPPLGP
                     EGSLLERARAGGIPIKLVPSLRRAINPWHDLRAYLAIRKSIKEFRPEVVHTHSAKAGI
                     LGRAAAWQLRVPVVVHTVHGAPFHPYQPAVAQKFVIACEKWAAAHCHAFISVADAMTD
                     LMVNAGIASRERFTTIYSGMDVTPFLDCDKHRAEMRRQLGYDEQDVVIGTIARLFHLK
                     GHEDILAAAEVIIPKFPQVRFLWVGEG"
BASE COUNT         1909 a         2359 c         2353 g         1858 t
ORIGIN      
        1 tttgagcggc caactggcaa atcgctgacc atttcgtggt tcctgccggc atgatctcac
       61 agaagaaccc ccgatatcgc gggcttcgca gaacgttcgt cgtcaattgg tgcggcaggg
      121 ctgcgtggag ttggctttcc acccatttca tctcggcctc actgccaacg gtaaacgtgg
      181 cgaaaatctc cggcggaagc tctgccaaaa agtcgtccca aatcctttct gatcctgagt
      241 tttgctcaag gtaatcttca aaatagggat tgcgataacc gtttggccgc tcaatgtagt
      301 aatcaaaacc gtggctgaat gtgtctccaa gcatggcggc aggaaacccc aattcccgaa
      361 gaacgatcag agtcttccgg agaacctcgt ccgaaaatcg ggccctgaat aaggtttcat
      421 gtgttttggg aatcttggtc agggcaccgc tcgaagtgac gaggggggtc tcaatctgaa
      481 ggattttgga aaagggcagg gcccggctat accttcgccc ggtcgccaag ataacctcca
      541 gcccggcttc ggcagcccgc gcaatggcct cccgtaccga tggtgagatt gcatccacgg
      601 tactgaccag cgttccatca atgtccagtg ccaagaaccg gtagcccatg atcgatcaca
      661 tttggcaaac actgccaatc ctggcaggtg gttattcccc tcaatcttgt cgtgaacggc
      721 ctccgagcac gtttcttcat cttaacaccc ttgccacttc ccgacagctt ctctggccga
      781 aggtagccgg taggcttctc cgcatacttt ccatattact cggtggggca caatcgtgtt
      841 cgccccgtgc cccgccgaac gaaccccttg cctcccatgg cgaggttgga ttatcggttc
      901 agtgcggcaa gatcccgcca ggcacggtcc cgctcccgct ctaaatagcc tcgcccctgg
      961 cggatactga cgtggtccca aggcagccgc gcttcaggac tccacggccg atgaagcgtt
     1021 tcttcgacgg gcagctcaag atcgtcgaag acgtcccacc aaatttgagg gttgaattgc
     1081 tcggcccaac cgtcaaagcg tgctccccgg cgccaagcct ccaggatcgc ctgccccatt
     1141 cgccggtccc ctcgcgtcat gatcccctcg agcaaactac tttccacggg atgacatttg
     1201 atctggacac tccgcatccg acaccgctgc cgcaaaaact ggtgcgcttg cgtaaagtat
     1261 tccctggtct gcatgggatg ccactgatag ggggtgtgag gcttggggac gaagttcgaa
     1321 acattagcga ccacagtcgc cggacggccc agaattgatc tgcccagccc tgaaatctcc
     1381 tgggcaagct ccacgatacc tgccaggtcg gcctcacgct ctcctggcaa gccacacaag
     1441 aaatacaatt tcacgcggtc aaaaccgctg cggaatgcct ctcgacagcc atcaatgagg
     1501 tcctcattgc gaatccgttt gccgatttgc acccgcatgt catccagcgc cacttcgggg
     1561 gcaagcgtca aacctgagtg gcgatccggc gtcaacattt gggtcaatac ccgcagttgc
     1621 tcgttgaccc gcaagctagg gaccgagatt gccacatggc gaggtgcgaa cacttctcga
     1681 agccgtgtga taagttcggc aaactgcgga taatcgctgg atgaaagtga taacaaggcg
     1741 atctcgttcg tcccggtatt acggtacgct tcttccgcag ccgcaaccaa ggtttctaca
     1801 cgacggtatc tcagcgggcg tttgatcgtg gtactctggc agaagcggca ccgccacgga
     1861 cacccccgca tgatttccat agcgatacgg tcctggacaa tttctacaaa gggaacgatg
     1921 ggggccgtag gcaatggaaa accgtccagg tcagcaagga ctgccggtga aatgacgtcc
     1981 gggatacccg gctctgcagg gcaaaccgcc ataagaccgg ttgatggttg aacctctgcg
     2041 tgataaaggc tcggcacata tacgtgaggg agttgtcgag ccatcttggc caatgcgtca
     2101 cggcggcttt ggctggtctg tcgggcttcc agccaggcgt tgcaaacagc gggcagaagc
     2161 tcctcgccat caccgatcac gaaaagatcg ataaaacaac tcatcggttc cggattctga
     2221 gcgcacggcc ccccggcaat gaccagcgga tcatttaggt ccctttcctc ggcaagcagg
     2281 ggaatccgtc ctaggtctaa caccgttagg acattggtgt agctaagctc gtattgaagg
     2341 gagaacccga taacgtcaaa acgatacaag ggcgtgaatg tctcaaggct atatagaggc
     2401 aagttccagt ggcgcaactc tgcctccata tcccgtccag gggcaaacac ccgctcgcac
     2461 gcccaactgg tccgccggtt catcgtatca tacaggacct gcaagccgtg attgctcatc
     2521 ccgatggcgt aggtatccgg ggtacaaagg caaaggtgtc cacagacctg agatgtgtcc
     2581 ttaacaacgg agttgagctc accgcccaag tattgagcag gagtctgcac ccggggtagt
     2641 acttttctta ttactttgtc gcgtagatcc gcgttgttaa ccactatgaa caacctccag
     2701 taaacctttt cccagccttg cgaagacccc ttacctcatt gttgtggaaa acgctcttga
     2761 gtcaaacacc gctatcttca accggaactt acctgggaga gtccctttct tcgtggatga
     2821 aaccgaagac gagtaactac aatggaagtg aggtgagcaa cataggtcag ccattgggac
     2881 atgaccaaag tgattgggcc gcttggcaaa agttgaacgc cctcaatgag gccaccgagg
     2941 ggagatggta taaccgtgat caatggatcg ggtgggtagc atgaaaacct ggttccaacg
     3001 tttgtcgttt gttgtcatcg tggtggcttt ctccccggtc tcaggtcgag cagagcaggg
     3061 gggcaggggc attctgccgt ttgaccggct tcgtcaggag atggttcgtc gcgacatcct
     3121 cgggggtggg atcaaaaacc cgcgtgtcat tgctgccatg gcatcggttc cgcgtcatga
     3181 atttgtgccg ccggagcagc gccgactcgc ctatcatgac atggcccttc cgattgggta
     3241 cggccaaacg atctctccgc cctttgttgt agcctacatg acggagcaaa ttgacccaca
     3301 accgaccgac cgggtgttgg aaatcggaac ggggagcggt tatcaagccg cgattctcag
     3361 tggtttggtg gccgaagttt acacgattga gatcgtcgaa ccccttgccc gacaggctga
     3421 ggccaccctc cggcgactgg gatacaaaaa tgtttttgtc aaagcgggtg acggttacca
     3481 ggggtggccg gaacatgccc cattcgacaa gatcattgtc acatgctcac cggaaaaggt
     3541 ccctcagcca ctgatagatc aacttaaaga gggtgggcgg atggtcatcc cggtagggga
     3601 acggtaccag caaacaatgt atctcctcaa aaagaccggc ggagaactcg tgaaggaatc
     3661 actcaggcca acgttgttcg tccctatgac gggcatcgcg gaatctcaac gcgaagttaa
     3721 accagacccg gcaaagccgc agttgattaa cggagatttc gaggagattc gcccggatgc
     3781 cccggaaccg cccgggtggt attactgcaa gcaatttgag ctccgacaga acgacggcgc
     3841 accttcagga agtgtttatc tggccctgcg aaatcgggaa ccggggcagc cggccagggt
     3901 tcttcaggga tttcccattg acgggcgcca ggtgcaggag atcctagtga cattttttgt
     3961 ccgagcacaa aacgtccgcc ctggtcgaaa ccctcaggag tcggcggggg ttattataac
     4021 cttttttggg gaagataggg cacctgttgg ggaagcaacg atcggtccca ttatgggaac
     4081 tttcccctgg cggcatgtat cgcgccgagt ccaagtgccc gccgcggcaa gggaagcgat
     4141 cttgggaatc ggcatgacgg gggccatcgg ggagctggcc atcgatcggg tggaaatctt
     4201 ggcaacctcc gagaagaagc cataacaatt acaccggcaa cagggaatgg tgcaaagccc
     4261 ttgggtaatt tccaccgatc acaaaggtta gcaaccactg ggctgtcggt ttccacacgt
     4321 gagtatgcta ctgcaattcg acccccatta cctcgtggaa gggtatagcc cgacatagcc
     4381 cgcataatcg gtaaactttg cccgaccact tgaaatcccc gttcctgcca gggtaaggtg
     4441 gataaaagtg acaaagattt cgtaggtttt ctcaggacgc ggtgtcactt agccggaacg
     4501 gccgaccttc ttgcgaaagg aaccaaacga tgcgaccgag aattctgatg gccttcgctg
     4561 cgttagggat tggcgtaggg tgtttgggag cgacatcgga ggcctgctgg cctcgtcatg
     4621 tctgcggtcc atggtatccg ggacctctta ccgctttggc ctgctgggcg ccatgttggt
     4681 cggtttgtga cgtctgtcag ccatatgatc catgctgtta cgatgatggt tgggtcctgg
     4741 gcgtccgccc cggccccatc cgccgtttga ttcttggtcc gtatcgctgg taccgtgttc
     4801 ctttcgggtg ctgtgacgtc tgtggcgaag tcgcatgtac atgcgaaccg gcgattgtca
     4861 cagggccagc actgacgccg acgccagctc aacctttctc cacaccggcg caaccaacac
     4921 cagctcagcc gagcccgacc cctgacgccc ttccgtcacc cagtccaact cccggcgccg
     4981 atatccctaa tcagccggcc ccatcgacgc cgcttgtcaa tcccacaagc tacacggtcc
     5041 ccacacggaa cgacagtggt ctactcacgg tctatgtccc tgcggaggcg aaggtcctta
     5101 tcaacggtct cccgaccaag agcacgggaa gccgtcggga atacgtctcg tatgggttga
     5161 aggaaggact ccaatatcgc tacgtcatta cggcccaagt ggaacaggac gggaaactgt
     5221 acgaagaaac ccgcgaagtc atcctcacgg cgggagaaaa gaagggggtc gcgttctcct
     5281 tccagttccc aacggagaat atcgcgggtc tttgatcaga agcaggttgg ataagagccc
     5341 ctcgttggca agccaaagaa agatcgaaag attaggaagc tcattagaaa acgacgctgt
     5401 ccatcacggc gtcgtttttc tttgttatcc catgtatgac acttgatttc cttcgaaaag
     5461 agggacaagt taggcagggt aggggggttc gcgccacaca aacggcggaa tatgatcaag
     5521 ttttcgacat aggtctcggc ggagaccaag gcgtgaacca ccatcttgca atcgttgtca
     5581 ataaacactc cagactgaca tttcgtagaa agcccttggc tgtttataaa cgtgctcgcg
     5641 ggagagatgt gtttttcatt gacggtctgc aaccagaccg tgaacgttcc ccgctaaggt
     5701 gtattttgaa gaattggatg gtattggagg actggttcgg tgtttaagac tttggcagtt
     5761 gttggcgcga ccggggccgt tggccggatc atggtccagc ttttggaacg aagttctctc
     5821 acgtttgaac gaatcaaatt ccttgcttca gcgcgctcga tcgggaaaac gattgagttt
     5881 cgaggagaaa aatatcccgt ccaggaactt cgcccggagg ccttcgacgg agttgatttg
     5941 gccgttagca gcacgcccga cgaggtggcc aaagaattca ttcccgaagc cgtccggcgg
     6001 ggatgtgtcg tcgtcgatga aagcgctgcg tggcggatgg acccaaacgt gcctctggta
     6061 atccccgagg tcaatcccca tgcggccttc aagcacaacg ggatcatcgc tagccccaat
     6121 tgttccacca cccagatggt ggtggccctc aaaccgttgc acgactacgg cagggttcga
     6181 cgcgtcattg tgagcaccta ccaggcggca agtggggccg gtgtcggtgg gacaaaagac
     6241 cttttcgaag ggacacgagc ctatttggag aataggcctt acgcgtacac aacctttgcc
     6301 catcctttag cattcaacct gattccccaa attgggtctc cccggcccaa cggctacacc
     6361 tccgaggaaa tgaagatggt cctcgaaacg cgaaagatca tggaggatga gtctattcag
     6421 atctgcgcga catgcgttcg cgtgcctgtg gccaactctc acagcgagtc gatcctggta
     6481 gaaacagaga agaagatcac cgttgagaag gccagggaac ttttctcgaa attcccaggg
     6541 attgtcgtca ttgataacct ggaagcgaaa caatatccat tgcctttaaa ctgttctggc
     6601 cgggatgagg tcttcattgg tcgtattcgg gaagacttct cttcgccgaa cgggatcacg
     6661 ttctggtgtg tgagcgataa tctccgcaaa ggtgccgcga caaacgccgt acagattgtc
     6721 gaacttctgt tgcggggacg gccctaacgc gggttccgcc cggaccaggt cccatgcgat
     6781 gcatcaggtt gacccttgct tacgatggca gcaacttcct gggatggcaa cgtcaagcaa
     6841 acggtcggac cgtccaaggg gttatcgagg ccgccatcga gaagatctcc ggaaaacgat
     6901 ctcgggtcct ggcaagtggt cgaactgatg ctggggtaca tgccctggga caagtcgctg
     6961 catttcggac ggaaagcaca ttgactgccg aaacgtgggg caaggccctc aacgctgtct
     7021 tgccccacga tattgttgtt cttgcggctg aggacgtccc gttgtccttc catcctatcc
     7081 gcaatgctca gcgaaaacgc tattgttatc tcctcgacga cggtgccgta cgtccggtga
     7141 ttttccggca gttcgtttgg cactatcctc gcccacttga tgtcgatctc atgcgcacgg
     7201 ccagtgaatt tctaattggc caacacgact ttgcggcttt tgagacgagc ggtgccccta
     7261 gaaaaacaac cgtccgcacc attttcgaac tctcagttcg ccgagtgcac ccgtatatgc
     7321 cttcggtcat cgcgattgag attgaggcag atggtttttt gtataatatg gttcgcaata
     7381 ttgtcgggag cctcgtagag attgcgcggg gtgcccgacc tgtggcgtgg ctgggcgagg
     7441 tcttggcttc tcgagaccgc cgccttgccg gaccaaccgc cccaccccaa ggcctgtttt
     7501 tggtatcggt cacctacgac aatttgccga ccttttcggc tgcaaacccg tggttcctcg
     7561 gctccttgag aatggtgcac gcggaaaacg agacccgcct tgaacaatag gggctcaccg
     7621 gggtatgaca cgatcgttat ggcatactgc gaaacatatt tagtgttttc agagggccga
     7681 aaccagatgc cgctctctcc tctacagtca gcacgcaatc gttgctcaac gacaggcagc
     7741 tcgttatgag agtccttcat attatcacac ggttgatcgt aggcggagca caagaaaaca
     7801 cgatcctcac gtgtgaagac ctcattcata gataccacga tgatgtgtta cttgtgaccg
     7861 ggccaccgct tggacctgaa ggaagccttc tcgaacgggc tagggcaggg gggattccga
     7921 tcaagctcgt gccgtcctta cggagagcca ttaatccctg gcacgacctc cgtgcctact
     7981 tggcaatccg aaagagtatc aaggagtttc gaccggaagt cgttcacaca catagcgcga
     8041 aagccgggat tttgggtcgg gcagcggctt ggcagttacg ggttccggtc gtggttcata
     8101 cggtacacgg tgccccgttt catccctacc agccagcagt tgcgcaaaaa ttcgtcatcg
     8161 cctgcgaaaa atgggcggcg gcgcattgcc acgccttcat cagtgtggcc gatgccatga
     8221 cggacctgat ggtcaacgca ggcattgcct ctcgagaacg cttcacgacg atctacagcg
     8281 ggatggacgt cacgcccttt ctcgattgcg acaagcatcg agctgagatg cgtcgccaac
     8341 ttggttacga tgagcaagat gtggttatcg ggacaatcgc gcggttgttt cacctgaagg
     8401 ggcacgaaga catcctcgcc gcggcggaag tcatcatccc gaagtttccc caggtgcggt
     8461 ttctctgggt gggggaggg
//
LOCUS       DAOPTR010000021         8236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_7256_length_8236_cov_3.406430, whole genome shotgun sequence.
ACCESSION   DAOPTR010000021 DAOPTR010000000
VERSION     DAOPTR010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 8236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8236
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7256_length_8236_cov_3.406430"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PKI05_00865"
     CDS             <1..193
                     /locus_tag="PKI05_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50773.1"
                     /translation="LAFGDDIRRFIEEHREELRWRVRPEPQVETQGEPPKESTVTTDR
                     SVASESGVNPDSLESQGCD"
     gene            440..1993
                     /locus_tag="PKI05_00870"
     CDS             440..1993
                     /locus_tag="PKI05_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50774.1"
                     /translation="MLTKDWRRPLAITVVVAWSTLVLGKAQKVKADEPIPVYQIAQRR
                     ERLPEIPPASAVCFSSRWFHPVNRDDPHDTFKAAEAFHATDFVWTYSLDPKFVGRLRQ
                     MGHRVFLAVNSMVPDPPDFRRRLRGRIVDLENRPVTAPWMRSWADPAWGCANSPEYRE
                     GFIEYAVRAVEAGATALQMDDPRMNMAAVAWGACFCPHCMESFRHFLKQRVSKETATE
                     WGIGNLDTFDYRQYLIERKAPVGDAFAKYDGGELKKLFTEFQTESVGQFFSYVRAEID
                     RRVGRHIVFSSNNYRGSWEFPYNLFELGMAELPQREATPMTLYERYREAAARGKHQIF
                     TLVPQTADGSEVPITRRTIAACYALGGHLIVPWDVYTGPQKPRYYGKPEDYADLYKFV
                     RDFAQWFDEYEEAAFVFDGFHDERYSGHPPLRVDATQVMGVVRAKPQDPAAPVVIHLV
                     SWQDTPSPFVIRIDPRQFPSGVSKHILLLLPGQAPRKITPTEHGNAEISVEIPALNPW
                     GVLLLGAGP"
     gene            2094..4301
                     /locus_tag="PKI05_00875"
     CDS             2094..4301
                     /locus_tag="PKI05_00875"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HOA50775.1"
                     /translation="MMTSVAKHRMSFGHAPARARLFILFLAFSLSPALGAAQIKILEF
                     QPDVPVCRAERLITLHLTLGNDGARDEQGTVELHLPPVVELIKGNRNTPFAFRDGAGE
                     LTLSWTVQASQEGRHELAVEVKPVSGSPVRQTLNLLFLPSVTIEKADRIPDPQPVATD
                     ILVGAHHCPLWEKDRPEIWRNVIRHPERTPALGFYCQEQPEVADWETKWAVEHGISFF
                     VYCWYRASQGGPVETRLSSAIHEALFHSRFQDKIRFAIMWENQRRGVAGIANEEDFLQ
                     NLLPYWIENYFRRPNYLIIDRKPLLFIYRPEYLIQDLGSVEKVAQAFAKARELCRQAG
                     FDGIYLLGEYRGTNPKHLQLMKDLGLDYTFAYVWPVPNNPSPEEAIRAQLDYIKKTQE
                     LNILPQVITVSLGWSGWRDEGSIWKLPPEDFERLMREAKAFAKTLPPEQLGSRIILLD
                     NWNEWGEGHYIGPHREFGFGYLDAVRRVFAGNAATHLDLLPEDIGLGPYDLPIRAYYQ
                     RERESRQKARTWVKPIVSDEALVAYWTFDENKDEVAAFDYSGHRLGGILHQIERVKGW
                     RGTALDCRGGSMIVPTDERLSDWAQMSVECWIRTDQPDQDNRWIINRVFGGAETSGFR
                     LGILRGRPSFQIPVTAWSHHLVGKEPLPQGRWVHIAGTFDGTTMRLYMDGHEVGRMER
                     SGSVRNNNRPIVVGNYDVDHAAHFVGELDEVRLYSRVLTPEEIASRAAQPAEK"
     gene            4387..5139
                     /locus_tag="PKI05_00880"
     CDS             4387..5139
                     /locus_tag="PKI05_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOA50776.1"
                     /translation="MGPIFGDLVTILLAGLVSGIICRQLRISGLVGFIIAGIMIGRGG
                     LNLIASHGKELEQIAQTGVLLLLFAMGIEFSLAELAGLSRHMVLAGTTQVLLVTVPLL
                     VVFLLLEVSIERAIVLSAAVAISSTVLAYRTLTEWGQASSPIGRRALGISLFQDLLLI
                     PSLLFMPCYSGARSTGLISALVLTGMAVVFTAAVPLAHYVFEKWIVPFLIRVKSVELT
                     MLFVLVILLMACWLAQVFGLPPALGALAAGSV"
     gene            5136..6104
                     /locus_tag="PKI05_00885"
     CDS             5136..6104
                     /locus_tag="PKI05_00885"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOA50777.1"
                     /translation="MSETRLTKQLDALVFPFRETFGVVFFVSLGSYIDWRILWRDPLW
                     FLIAIPLLLALKSVAGGVALRISGLTWRTSLGAASLLVPMSELSFVLLSEAARSQILT
                     GTEYERMLMVALASMIVSPELFRIGLRVLRSDSSKRPTSMGFDVPSVDVNKAIVVGLG
                     PIGSMVASQLELRGMDVGLIDQSPVNLQPFAQQGFRTVCGDARDPEILLRAGGQEAHL
                     AMICVPDDTVAGTITRNLRQINPNCTIAVRCRYQSSRKTLMKAGAQIVISEEAVTSEV
                     LRNFIMTSVFNLSGESPLASSSPPNTPARPDSLSTGSTPEGKKPEA"
     gene            complement(6920..>8236)
                     /locus_tag="PKI05_00890"
     CDS             complement(6920..>8236)
                     /locus_tag="PKI05_00890"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOA50778.1"
                     /translation="GVIPYQTMDPAYLVATEKPPQIAETNDELTRRVADLEKALAQIK
                     EKEQAAAKKSAGQMSVNLSGRFFIDSVFFGQSDGSMAAFGDAQDTVQLRAARLGAFGK
                     GFDVFDYQIVADFASRDGSNSQRTSLKDAYIGVDDLPWLGRVQVGHFKEPFSLEELTS
                     ARFITFMERASFISLFVPSRNLGIAAFRVSENERMTLAFGGFREVGEKPPYVADDDGG
                     YAFTARVTYLPWYDEATQGRGLFHLGLGYSFRDVDNPMRQLRARPEVNEGPYLLDTGV
                     FGNVEDYQLLNPEMAFVYGPFSVQSEYLAAFYNRAGGEDPTFHGMYVQTSYFLTGEHR
                     SYNRQKGVFERVKPFENFFRVRTSDGNVETGLGAWELAYRYSWLDLDDKDVLGGTMAD
                     HSFGINWYWNPYMRLMFNYVHAQVSPPRDATKADFNVFQFRAQVDF"
BASE COUNT         1818 a         2236 c         2263 g         1919 t
ORIGIN      
        1 tttggcgttc ggagatgaca tccgccgctt tattgaggaa catcgagagg aattgcgctg
       61 gcgcgttcgt ccggaaccgc aagttgagac acaaggcgag cctccaaagg aaagcaccgt
      121 cacaactgac aggagcgtgg catcggagag cggagtaaac ccagattcgt tggaatctca
      181 aggttgcgat tgatttttag ccgcagtgct ccacccaaac gattttggtg gaaggacgcg
      241 acctttgctc aaagcgattc tttcccatca agaaccaagg gttcctcggg tggtggcgtc
      301 aaccgaccga tctccgacct gttgaatgag gccgggatct accaacattg ggacaggtcc
      361 attctcagag tgtcccgaag tctcctttac atccctttac caaaacttcg acgaaaagac
      421 ccgaaagggc ttgatgaaaa tgctgacaaa agattggcgt cggcccttgg caatcacggt
      481 ggtggtagcg tggtccacgc ttgtcctggg taaagcacaa aaggtcaagg cggacgagcc
      541 tattccggtt tatcagattg cccagcgtcg ggaacggttg ccggaaattc ccccggccag
      601 tgccgtgtgc ttttccagcc gttggtttca ccccgtgaat cgcgacgatc ctcatgacac
      661 tttcaaagcg gccgaggcgt ttcatgccac cgattttgtt tggacctatt cgcttgatcc
      721 caaatttgtg ggacgtttga gacaaatggg ccatcgtgta ttcctcgccg tgaattcgat
      781 ggtcccagac cctccggatt ttcgtcgccg cctccgcggg agaatcgtcg atcttgaaaa
      841 tcgtccggtg acagctccct ggatgcgttc ctgggccgat ccggcttggg gttgcgcaaa
      901 cagcccggag tatcgagaag gcttcatcga gtacgcggtt cgcgccgtcg aagcgggggc
      961 aacggctcta cagatggacg atcctcgaat gaacatggca gccgttgcct ggggtgcgtg
     1021 tttctgtccg cattgcatgg agagcttccg acacttcctc aagcagcggg tatcaaagga
     1081 gacggctacg gaatggggaa tcggcaatct cgatacgttc gactatcggc agtacctgat
     1141 tgagagaaag gcccctgtag gcgatgcctt cgcaaaatac gatggtggtg agttgaaaaa
     1201 gctgtttact gagtttcaaa cggagtctgt ggggcagttc ttctcctacg tgcgggcgga
     1261 aatcgatcgg cgggttggtc gccacatcgt cttctcttcc aacaactacc gggggagctg
     1321 ggaatttccg tacaacctgt ttgagctggg gatggccgaa ctccctcagc gcgaggcaac
     1381 tcccatgaca ctatacgaac gctaccgcga agccgccgcc cggggaaagc atcagatttt
     1441 cactcttgtc ccacaaaccg ccgacggctc agaggtcccc atcacccgac ggacaattgc
     1501 cgcgtgttat gccctgggtg ggcatctcat tgtaccttgg gatgtttaca ctggtcccca
     1561 gaagccgcgt tactacggca agcccgaaga ttatgccgac ctttacaagt tcgtacgaga
     1621 ttttgcccag tggtttgacg aatatgaaga ggcggccttc gtcttcgatg gctttcacga
     1681 tgagcgatat tctggccatc cacctctgcg cgtggatgcg acacaagtga tgggtgtggt
     1741 tcgggcaaag ccacaagacc cagccgcccc ggtggtcatc catttggtca gttggcaaga
     1801 cactccctct cctttcgtga tccgcattga ccccaggcag tttccaagtg gggtatccaa
     1861 acacatattg ctcttgttac ccggccaggc ccctcgaaaa atcacgccca ccgagcatgg
     1921 caacgccgag attagtgtcg agatacccgc cttaaatcca tggggagttt tgctgctcgg
     1981 agcgggaccg taggttaatc ctttaatgtg gttccgctgt ggggtagggg atatcatttt
     2041 ctctaagtgt gaagccggcc attcaagaat tgcagtctca aaggaacgcg atcatgatga
     2101 cgagcgtggc gaaacataga atgagctttg gtcatgcccc cgctagagcg cggttgttta
     2161 tccttttcct tgccttttca ctaagccccg cactcggagc ggcgcagatc aagattctag
     2221 aatttcagcc ggatgtaccg gtctgtcggg cagaacgact tatcactttg cacctgacac
     2281 tgggcaatga cggcgctcgt gacgagcagg gaactgtcga acttcatttg ccccctgttg
     2341 tggaacttat caaaggaaac cgcaacaccc cgttcgcgtt tcgcgacgga gcaggggagc
     2401 ttaccctgtc ttggacggtg caggcatcgc aggaaggccg gcacgaattg gcagtcgagg
     2461 tcaaacccgt aagtgggtcg ccggttcggc aaacacttaa cctcctcttc ttgccatcgg
     2521 tcaccatcga aaaagcggac agaatcccgg atccacagcc cgtggccaca gacatccttg
     2581 tgggtgcgca tcattgtccc ctgtgggaga aagataggcc cgaaatctgg agaaacgtca
     2641 ttaggcatcc cgaaaggaca ccggctctgg ggttctactg ccaggagcag cccgaagtcg
     2701 cggactggga aacaaaatgg gccgtcgaac acggtatttc gtttttcgtt tactgttggt
     2761 accgtgccag ccaaggcggg cctgtggaaa cccgactgtc cagtgccatt cacgaagccc
     2821 tatttcattc gcgattccaa gacaagataa gatttgccat catgtgggaa aatcagcgtc
     2881 ggggcgttgc aggaattgcc aacgaggagg acttcctgca aaacctctta ccgtattgga
     2941 tcgaaaatta ttttcgccgg ccaaactatt tgatcattga cagaaagcct ctcctcttca
     3001 tttatcgccc agagtatctc atccaggact tgggcagcgt tgagaaggtc gcccaggcct
     3061 ttgccaaggc ccgtgaactc tgccgtcaag ctggatttga cgggatttat cttttgggtg
     3121 aataccgcgg aacaaacccc aaacacctgc agcttatgaa agacctgggg cttgattaca
     3181 cgtttgccta cgtgtggcca gtccccaata acccgagtcc tgaggaggca atccgcgccc
     3241 agctggacta catcaagaag acgcaggaat tgaatatctt gccccaagtg attacggttt
     3301 ccctgggatg gagcggctgg cgcgacgaag gttccatctg gaaactgccg ccggaggatt
     3361 ttgaaaggtt gatgcgggaa gccaaagctt tcgccaagac cttgccccca gaacaattgg
     3421 gaagtcgcat catcttgttg gataactgga acgagtgggg cgagggacat tacattggcc
     3481 cccatcgaga attcggcttc ggttatctgg acgctgtccg tcgtgttttt gccggcaacg
     3541 ctgcaacaca tcttgatctc cttcccgagg atatcggcct tggtccctat gatttgccga
     3601 ttcgtgcgta ttaccagcgg gagcgggaaa gtcggcaaaa ggcccgaact tgggtgaaac
     3661 cgatcgtgtc tgacgaagcc ttggtcgcct attggacatt cgacgaaaac aaagatgagg
     3721 tggcagcctt tgactattcc gggcatcgcc tgggagggat actccatcag atcgaacgcg
     3781 tgaaggggtg gcgggggacg gctctcgatt gccgcggagg ctccatgatc gttcccaccg
     3841 atgagcggct ttcagactgg gcgcagatgt cagttgaatg ttggattcgc accgatcaac
     3901 ctgatcagga caataggtgg atcatcaatc gggtgtttgg cggtgccgag acatcgggat
     3961 ttcgcttggg gattctccgg ggaaggccca gttttcagat tcccgtgacg gcttggagtc
     4021 atcatttggt gggaaaagag cctttacccc aaggccgctg ggtccacatt gctggcacgt
     4081 ttgatgggac cacgatgcgc ctctatatgg acggtcacga agtcggtagg atggagcgat
     4141 ccgggagcgt ccgcaacaac aaccggccga tcgttgtggg caactatgac gtggaccacg
     4201 cggcccactt tgtgggcgaa ctggatgagg tacgtctgta tagccgcgtg ttgacgccgg
     4261 aggagatcgc ctcacgagcc gcccagcctg cggaaaaatg agccagacaa aaaggaattt
     4321 aggctagaat gggtatattc ccgacaaaaa gtctcttcgt gcatcgtgag ggcgaaaggt
     4381 tgtagcatgg gacccatttt tggagatctt gtcacgatcc tgctagccgg tcttgtctca
     4441 gggattatct gccggcaatt gcggatttct ggcctcgtcg gttttatcat cgcgggtatc
     4501 atgattggcc gcggagggct gaatcttatc gccagtcatg gcaaggaatt ggaacaaatt
     4561 gcccagacgg gagtgctttt gcttcttttt gccatgggaa ttgagttctc actggctgaa
     4621 ttggccggcc ttagtcggca catggtcctg gccggtacca cacaggtgct cctcgtgacg
     4681 gtccccctcc tcgtggtgtt tttgctttta gaagtttcca ttgaaagagc catcgtattg
     4741 agtgcagcag ttgccattag ctcaacagtg ctcgcttatc gcacacttac ggagtggggg
     4801 caagcaagct cgcccatcgg tcgtcgagcg ctaggcattt cattatttca ggacctccta
     4861 ctcattccct cgctcttgtt tatgccgtgc tattccggcg cccgctccac ggggttgatt
     4921 tctgctttgg ttctcaccgg catggccgtt gtcttcactg ctgctgtgcc cctcgctcat
     4981 tatgtctttg agaaatggat tgtcccgttt ctgattcggg ttaagagcgt tgagctaact
     5041 atgcttttcg tcctggtcat tctcctcatg gcttgttggc tggctcaggt attcggtttg
     5101 cctccggcgc taggtgccct cgcggccggg tcagtttgag cgaaacacgc cttacgaaac
     5161 aactggacgc actggtcttt ccgtttcggg agaccttcgg tgttgtcttt tttgtgagcc
     5221 tcggtagcta cattgactgg cgaatcctct ggcgagatcc cctttggttc ctgatagcca
     5281 ttcctttact cctggccctc aagagcgtgg ctggcggggt cgccttgcgg atttccggcc
     5341 tcacctggag aacctcactg ggagcggcat cgctgctggt acccatgagc gagctctcgt
     5401 ttgtcctcct ctccgaagct gcccggagcc agattctcac aggcactgaa tacgaaagga
     5461 tgctaatggt cgcactagca tctatgatcg tctcacccga actttttcgg atcggcttgc
     5521 gtgtgttgcg cagcgactcg tcaaaacgac cgacgagcat gggattcgac gtcccgagcg
     5581 tggatgtcaa caaggccatc gtggtgggtc ttgggccgat tggcagcatg gtggcgtcgc
     5641 aactggaatt acgcggtatg gacgttggcc tgatcgacca aagtcctgta aatctccaac
     5701 cgtttgcgca gcagggattt cggaccgtgt gcggcgatgc ccgcgatccc gagattctcc
     5761 ttcgggcagg tgggcaagag gctcacctag ccatgatatg tgtcccggat gacacggtgg
     5821 ccggaacgat cacacgcaat ctccgccaaa tcaaccccaa ctgcacgatt gccgtgcggt
     5881 gtcgatatca gtcttcccgc aagaccctca tgaaggcagg tgcccaaatt gtaatcagtg
     5941 aggaggccgt gacgagcgag gtgctcagga acttcattat gacgtcggta ttcaatcttt
     6001 ccggagaatc tccacttgcg agtagctccc cgccaaacac tcccgcaagg ccggattctt
     6061 taagcacagg cagcacgccg gaagggaaaa agccggaggc atgagggaat cgtggttcaa
     6121 ttaaccccgg taatacagta cttggcgatg ttattctagc ggcggaagtg gagctggggt
     6181 cgtcatctgc gccgcggaga ctggcacgag ctgtgaagac ggcagaattt ctggacttga
     6241 tacgttttcg gaagggcccg gacttggtaa gtcgcactct gatatatcac acgcaccgtt
     6301 ccgtcggatt cctaggcact ctgcacagac gtattgaacc acggtgcgat agacgggttt
     6361 ctcgaccacg tattctttct taaccagttt cttgactgcg cgcggctgac ccggtcgcgg
     6421 cgcaatgatc ggcaatggct tttctgtaag ttgctcaatc caacgagaaa gcacgccctt
     6481 gggttggcag gcaccacttt gtgagtcgca cgcttggccc tcacagccca gtgagggatc
     6541 gcacgtccca cacctgccgc cgtgttgatt gccacaccca cacgggcacc ggggcagcat
     6601 ggtgcataca tcgtgcacct ccacttccca acaatgcttg gtgatcttct caataccaca
     6661 ttgaacttga caaatttttt gctggcatgg ccccggcttg ccacaaatgg cgcatcggcc
     6721 cggtgaaccg taaacgtcgg tttgcgacga ttcaagcgtc cagccggtac tggcaataag
     6781 agttacaaac accacgcaag ccgcagcagt tttcatgttc atgcctgcat ctctctttta
     6841 tctccaaacg tgagggaaac ctcgcgtccg tcgctttgct tggtattctt gaaactatcc
     6901 tgctggtcga tggagacgct cagaaatcta cttgcgcacg aaattgaaac acattgaagt
     6961 ctgcttttgt ggcatcgcgg ggtggactga cctgcgcatg cacatagttg aacatgagcc
     7021 tcatgtaggg attccaatac caattgatgc caaaactgtg gtccgccata gtcccgccca
     7081 agacatcctt atcatccaag tctaaccacg aataacgata ggctagctcc caggccccca
     7141 agccggtctc cacgttgccg tcggatgtcc tcactcggaa gaaattctca aaaggtttga
     7201 cacgctcaaa cacacctttt tggcggttat aggatcgatg ttcgccggtg aggaaataac
     7261 tcgtttgaac gtacatgcca tgaaatgtcg gatcctctcc gccagctcgg ttgtaaaacg
     7321 ccgccagata ctcagactgg acagagaaag gtccataaac aaacgccatc tccggattga
     7381 gaagctggta atcttccaca ttcccgaaaa cgcccgtgtc aaggaggtat ggcccttcgt
     7441 tcacttcggg acgtgcacga agctgccgca tggggttatc gacatcccgg aagctatagc
     7501 caagaccaag gtggaacaaa cctcgccctt gagtcgcttc gtcataccag ggcaagtagg
     7561 tcacgcgggc agtgaaggcg tagcccccat cgtcgtccgc cacgtaggga ggtttttcac
     7621 caacctcccg gaatccgccg aaggccagag tcattcgttc gttttcggaa acccgaaagg
     7681 cagcgattcc caaattgcga gacggcacga aaagggaaat gaaactggca cgctccatga
     7741 aagtgataaa acgagctgac gtcaattctt caagactaaa gggctctttg aaatgaccga
     7801 cctggacacg gcctaaccaa gggaggtcat caacgccgat atatgcgtcc ttaagcgacg
     7861 ttcgttggga gttggaacca tcccggctgg caaagtctgc cacaatctga taatcgaaaa
     7921 cgtcgaaccc tttgccaaat gcccccaacc gcgcggctcg aagctgaacc gtgtcctggg
     7981 cgtcgccaaa cgcagccata ctgccgtcgc tctgaccaaa aaacacacta tcgatgaaga
     8041 accggccgct cagattcacc gacatctggc cagcggattt ctttgctgcg gcctgttcct
     8101 tctctttgat ctgagcaagg gccttttcca gatccgccac ccgccgcgtt agctcgtcgt
     8161 tcgtttccgc aatttgaggc ggcttctcgg tggcaaccag ataggcaggg tccatagttt
     8221 ggtaaggaat tacgcc
//
LOCUS       DAOPTR010000022         8216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_7287_length_8216_cov_3.729813, whole genome shotgun sequence.
ACCESSION   DAOPTR010000022 DAOPTR010000000
VERSION     DAOPTR010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 8216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8216
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7287_length_8216_cov_3.729813"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1722
                     /locus_tag="PKI05_00895"
     CDS             <1..1722
                     /locus_tag="PKI05_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008680009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOA50779.1"
                     /translation="IEYLANAKILQIKMAQGAKPGEGGQLPGHKVTEEIARLRHAMPG
                     VSLISPPPHHDIYSIEDLAQLIYDLKCANPEAMVSVKLVAEVGVGTVAAGVAKGNADE
                     ILISGHDGGTGASPWSSIKHAGIPWEIGLAETQQTLVLNGLRDRVLVQVDGQMRTGRD
                     VVIGALLGAERFGFGTAALVSLGCVLMRKCHLGTCPVGIATQMPELRARFAGKAEYLI
                     RYLLFVAEEVRRWMAQLGFRRFDDMVGRVDRLRVRRAIDHWKAKGLDFSAVLAPPPVP
                     EGTPLRRVRPQTDKHQDHLDRVLLPQVQAAIDNMQPMRLEMPIRNIHRTVGTTLSYHI
                     VKKHGSRGLPDGTIHLVFHGSAGQSFGAFLAPGITLELIGDANDYLGKGLSGGRIVVR
                     TPPGSPFDPAENVIVGNTVLYGATSGEVFINGLAGERFAVRNSGATAVVEGVGDHGCE
                     YMTGGVVVVLGKTGRNFAAGMSGGIAYVLDEYQLFDTLCNLDLVELESVWQPQDQQQL
                     RQLIERHYAWTGSEQAKRLLDKWPESVGKFVKVIPIEYRKALERIREREQVRAEQLPA
                     TEEVFSG"
     gene            1715..3142
                     /locus_tag="PKI05_00900"
     CDS             1715..3142
                     /locus_tag="PKI05_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HOA50780.1"
                     /translation="MGNPTGFLQYPRVEVDHRPVAERIRDWHEIDRPLAERVLNEQAA
                     RCMDCGIPFCHMAGCPVKNRIPEFNDLVYRGRWREAAQNLHSTNNFPEITGRVCPAPC
                     EPACTLSINSQPVTIKHIEYQIAEKAFAEKWVRPLKPTHRTGKRVAIVGSGPAGLAAA
                     QQLCRLGHEVVVFERSDRIGGLLRYGIPDFKLEKHVIDRRLEQMVAEGVHFEPNVWVG
                     RDITADQLRRDFHAVLLAMGAGKPRPLTVPGADAKGVHFAMDYLTQQNRRVAGDPIPT
                     SGPNSIYARDKHVVVIGGGDTGSDCVGTAIRQGAKSVLQIEILPKPPEGKNPETPWPL
                     WPRIMRTSTSHEEGCTRRWSVLTKALTVHNGQITHLHGCEVEWVRGPRGWEMREKPNT
                     DFTVPADLVLLAMGFLHVEHDGLIEQFGLELDNRGNVRVTRWMTSVPGVFAAGDTVKG
                     ASLVVHAINHGREAARAVDKYLQSL"
     gene            3235..4272
                     /locus_tag="PKI05_00905"
     CDS             3235..4272
                     /locus_tag="PKI05_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HOA50781.1"
                     /translation="MQRRHFLETASALICSIPFGFSVSFAEETSPKTEWPDAFTQNKR
                     LGRGVNIIGYDPLWRDRSRARFREEFFPLIRQAGFQHVRINLHPFRDNPQGGEGAGLR
                     PEYLETMDWAVDHALKAGLLVVLDFHEFTAIGRNPDKLKDRYMACWQVIAERQKNRPP
                     EVLFELLNEPNSAMTPELWNQWSREALALVRRRNPKRTVIIGPGQWNNIAALDKLELP
                     ADDPNIIVTVHYYSPFDFTHQGASWAGKADKVGIHWGSEEEKAAVRADFDRAQAWAER
                     HKRPLYLGEFGVYDKAPQEDRVTWLQFVTREAERRGWSWAYWQFDGDFILYDIPKNQW
                     VEHILKAIIPE"
     gene            complement(4421..4891)
                     /locus_tag="PKI05_00910"
     CDS             complement(4421..4891)
                     /locus_tag="PKI05_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50782.1"
                     /translation="MNTDRADGRFAVIGDITRDHLAGLSIRRKPYQGTTEPAVARSLD
                     EQGEIMLRRIVLATLLAVLIGTFFQQVASAQQAYGRQWGRTYSTQDWQRFYHYPYVYY
                     PQNYWGPDYYRSSEDLYYRYPPEMRIPVYNRNWQNFYPEPRRYYQGHHFIMDIF"
     gene            5070..6098
                     /locus_tag="PKI05_00915"
     CDS             5070..6098
                     /locus_tag="PKI05_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50783.1"
                     /translation="MARVPRENDALPWPGPRLGPIWVEWEIITAATHIHGGFALFYPT
                     RWIDHLLRARIMKDILGPKKPGRSLHAEISVDGQDWQSVITLAKNTIITLCTTALKCG
                     DRQLRVTSPWREVCRFDDRQVSHREYERRLSGGIRHQRQLTLCPRDNFFLFAETFLGS
                     RVTSWEYKLEIGLSRNTAVHPLPETQELELAVGDYAGAILPLAVPEWKSLTPQPGLVF
                     GGDHIVARYATTGRAFFFPLCFLFWKDKRAAGNPPSAESSANGQPLPVTWRQLTVSEC
                     LRKVSADEAVGFRFQLEKRQWLLYRSLTPPANRAVLGHNLVSEFLLARFTPKGQVIPL
                     VEVELDEE"
     gene            complement(6141..>8216)
                     /locus_tag="PKI05_00920"
     CDS             complement(6141..>8216)
                     /locus_tag="PKI05_00920"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50784.1"
                     /translation="PQVHYDGQKILFSYRKGGTHPFHLYEINVDGTGLRQLTDGPDDD
                     IEPTYCPDGSIIFCSSRCHRFVNCWYTRVAILYRCDADGKNIRPLSSNNDHDNTPWML
                     PDGRVLYMRWEYVDRSQVHFHHLWTMNPDGTFQTVFFGNMYGGVAMLDAKPIPNTNKV
                     VVSWSPGHGRPEHLGPVAVVDPSLGPDRQDAVKILGKERNWKDPYAISEDCFLVAHPR
                     GLFVMDGEGTAELIYQLPPEEQKTFQCHEPRPIVPRPRERIIPPRVDLTKATGTLVLE
                     DIYRGRNMEGVPRGAVKKLLVLKQLPKPVNFSGGMEPLTIGGSFTLAEIVGEVPVEPD
                     GSANFEVPALQSLFFVALDENDIAIKRMHSFVTLQPGEVTSCVGCHEVRTHAPHAVST
                     DLLALRHPPHQIQPISDVPKVLDFPRDIQPILDRHCVACHQPDRREGKIDLTGDHTAR
                     YSISYWTIRVHNLVSDGRNEPRGNRPPYSYGSAASRLMKLIDGSHYGAKLSPLEVKTI
                     RLWIETSACYPGTYAALGCGFYPVQLPWGELGQRCGNCHMGEIQTAEGPKKSLIFRGG
                     FPGATEPLSNLDRPEKSYILLAPLAKAAGGLELCGKPIFADTQDPLYQKTLAAIQDAH
                     RRLQEGKRFDMPGFRPNQHYIREMQRFGFIPKDLGPNDPIDYYKVDRDYWDSFLYQPM
                     KAGTLVTGE"
BASE COUNT         1779 a         2260 c         2373 g         1804 t
ORIGIN      
        1 atcgagtacc ttgccaatgc caagattctg caaattaaga tggcccaggg ggcaaagccg
       61 ggtgaggggg gacaattgcc cggtcataag gtgacggaag aaattgcgag attgcggcac
      121 gcgatgccgg gcgtgtccct gatctcgcct ccgccccacc acgacattta ttccatcgaa
      181 gacctggccc agttgattta cgacctaaag tgtgccaacc ccgaggccat ggtctccgtc
      241 aagctcgtgg ctgaggtagg cgtgggaacg gtcgcagcag gtgtggccaa gggaaatgcc
      301 gatgagatcc tcatcagtgg ccacgacggg ggcaccgggg ccagtccgtg gtcttcgatc
      361 aagcacgccg gtattccttg ggaaatcggg ctggcggaga cgcaacagac cctagtgctc
      421 aacggtctcc gcgatcgggt actggtccaa gtggatggtc aaatgcggac cggccgcgac
      481 gtcgtcatcg gggccttgct cggcgccgag cgatttggtt ttgggacggc ggccttggtc
      541 tcattgggtt gcgttctaat gcggaaatgc cacttgggga cttgtcccgt ggggattgcc
      601 acccaaatgc cagagttgcg agcacggttc gcgggaaagg cggaatatct tattcgctat
      661 ctcttgtttg tggcggaaga agtccgccgc tggatggccc aactgggctt tcgacgtttt
      721 gacgacatgg ttggtcgggt cgatcgcctc cgcgtgcgtc gcgctattga tcactggaag
      781 gcgaaaggtt tggacttttc tgcggtgctg gccccacccc cagtgccgga aggcacgccc
      841 cttcgccgcg ttcgtccgca gaccgacaag catcaagatc atttagatcg tgtgcttttg
      901 ccgcaggtcc aagcggccat tgacaacatg cagcctatgc ggctggaaat gcctatccgc
      961 aatatccatc gcactgtggg gacaacgctg agttatcaca tcgtgaagaa acacggcagt
     1021 cgcggactcc cggatgggac aattcacttg gtatttcacg gttctgccgg acagagtttc
     1081 ggggcctttc ttgcacctgg gatcacactg gagctgattg gtgatgccaa tgattattta
     1141 gggaaagggc tttccggtgg gcggattgtc gttcgaacgc caccaggcag tccgtttgat
     1201 cccgcagaaa acgtcatcgt gggcaacacg gtgctttatg gggccacttc gggcgaagtc
     1261 ttcatcaacg gcctagccgg cgagcggttc gctgtccgca acagtggggc aacggccgtg
     1321 gtggagggtg tcggcgacca cggttgcgaa tacatgacag gcggcgtggt ggtggtcctt
     1381 gggaagacag ggcgaaactt tgccgcgggc atgagtggcg ggattgccta cgtgctcgat
     1441 gagtatcaac ttttcgacac attgtgtaat ctcgatctcg tggaactaga gtccgtatgg
     1501 caaccccaag accagcagca acttcgtcag ttgatcgaac ggcattacgc ctggaccggg
     1561 agtgagcagg ctaagcgatt gctcgataag tggccggaat ccgtgggaaa gttcgtcaag
     1621 gtcatcccca tcgagtaccg caaggcattg gaacgtatcc gcgaacgaga acaggtccgc
     1681 gccgaacaac tcccggcaac agaggaggtt ttcagtgggt aatccaaccg gttttctaca
     1741 atatccgcga gtagaagtgg accatcggcc tgtggccgaa cgcatccgcg attggcatga
     1801 gattgatcgt ccgctggctg agcgggtcct gaacgagcag gcggctcgct gtatggactg
     1861 tggcatcccg ttctgccaca tggcggggtg cccggtgaaa aatcgtattc cggaattcaa
     1921 tgacttggtc tatcggggtc gatggcggga agcagctcag aacctgcatt ccacgaacaa
     1981 ctttcctgag attacaggcc gggtatgccc tgccccctgt gagcctgctt gcacgctgtc
     2041 catcaattcc cagccggtga cgatcaaaca tatcgaatac cagattgcgg aaaaggcgtt
     2101 tgccgagaaa tgggtccgtc ccctcaaacc gacgcaccga acaggcaagc gggtagccat
     2161 tgtgggctcc ggtcctgccg gtttggctgc tgcccaacaa ttgtgtcgcc taggacacga
     2221 ggtcgtcgtg ttcgaacgct cggaccgcat tggcggactg cttcgctacg gcattcccga
     2281 tttcaagcta gaaaagcacg tcatcgatcg tcgcctagag caaatggtgg cggagggcgt
     2341 ccattttgag ccgaacgttt gggtgggacg cgatatcacg gctgaccaac ttcgacgaga
     2401 ctttcatgct gttctcctgg ccatgggcgc tgggaagcca cggccactca ccgtccctgg
     2461 cgctgatgcc aagggtgttc actttgccat ggattatctc acccaacaga accggcgggt
     2521 ggcgggtgat ccgatcccta ccagcggccc aaacagcatt tatgcccggg ataagcacgt
     2581 cgtcgtcatt ggtggaggtg atacgggaag tgactgtgtg ggcacagcca ttcgtcaagg
     2641 cgccaagtct gtgcttcaga tcgagatact ccccaagcct cccgagggca agaaccccga
     2701 aacgccctgg ccactctggc cgcggattat gcgaacgtca acgtcccacg aagaaggatg
     2761 tacccgccga tggagtgtct tgaccaaggc acttactgtc cacaatgggc agatcaccca
     2821 tctgcatggg tgcgaggtgg agtgggtccg cggtcctcgc ggctgggaga tgcgggaaaa
     2881 accaaatacc gacttcaccg tgcctgccga tttggtcctc ctggccatgg ggtttttgca
     2941 cgtggagcac gatggactga tcgagcaatt cggccttgag ctagacaatc ggggtaacgt
     3001 ccgcgtgaca cgctggatga cctccgtccc tggggtcttt gccgcaggtg ataccgtcaa
     3061 gggggcttcg ctcgtggtgc acgcgatcaa tcatggtcgg gaagccgccc gcgctgttga
     3121 caagtaccta cagtccctgt aatgcttatc ttgattcaat tggtggcaac ccccggtatt
     3181 gccatcgttc gtaaacctcc caggcgatgg ttttttcctt ccttgaggtg aactatgcag
     3241 cgacgacact tcctcgaaac ggcttcggcg ttgatctgct cgattccttt tgggttctcg
     3301 gtgagtttcg cggaagagac atctccgaag actgagtggc ccgatgcctt tactcaaaat
     3361 aaacgcctgg gccgaggcgt gaatattatc ggctatgatc cgctgtggcg ggatcgttcg
     3421 cgggcaagat tccgcgaaga gtttttcccg ctcattcgac aagcgggctt ccagcatgtg
     3481 cggatcaact tacatccgtt tcgcgacaat ccccagggtg gtgaaggggc gggattgcga
     3541 ccggagtacc tggagaccat ggattgggcc gtggatcacg ccttaaaggc cggcctcctc
     3601 gtggtattgg actttcacga attcacggcc atcggtcgaa atcccgacaa gcttaaggac
     3661 cgttacatgg cctgctggca ggtgatcgcc gaacggcaga agaaccgccc gcctgaggtc
     3721 cttttcgagc tgctcaatga acccaactcc gctatgactc ccgaactgtg gaatcaatgg
     3781 tcgcgagagg ccctggctct ggtccgccgc agaaacccca aacggacggt catcatcggc
     3841 cccggccagt ggaataacat cgcagccctc gacaaattgg aactcccggc ggacgatccg
     3901 aatatcatcg tcaccgttca ctattacagt cccttcgact tcactcatca gggggcatcc
     3961 tgggcgggaa aggccgataa ggtcggcatc cattggggca gcgaggaaga aaaagcggcg
     4021 gtccgggcgg acttcgatcg ggcccaagca tgggcggaac gtcacaagcg gcccctctat
     4081 ttgggtgaat tcggtgttta cgataaggcc ccgcaggaag accgcgttac ttggctgcaa
     4141 tttgtcacgc gagaagcgga acgccgaggg tggagctggg cctattggca atttgatgga
     4201 gattttatcc tgtatgacat ccccaaaaac cagtgggttg agcacatcct taaggccatc
     4261 ataccggagt aatatcccgg tccgaatggc gcgtcggccc aaaggctttt aaccaatgaa
     4321 aaaacctgcc cgagtatcgt acttaggcag gttttttgtt ttcgccggtt ggcaagcgaa
     4381 ttgtttactg gcgagtactc gcctggcctg cctcgtgtcg ttagaagatg tccatgatga
     4441 agtggtgtcc ctggtagtag cgtcgcggtt ctgggtagaa attctgccag ttgcgattgt
     4501 aaaccgggat tcgcatttca ggtggataac gataatagag atcttccgag ctgcggtagt
     4561 aatccgggcc ccaatagttt tggggatagt agacatacgg gtaatgataa aaccgctgcc
     4621 agtcttgggt gctgtaggtg cgtccccatt gtcgcccata agcctgctgg gcggaggcca
     4681 cctgttggaa gaaggtacca ataaggacag ctaataaagt cgccaagacg atccgtcgca
     4741 acatgatttc cccctgttcg tccagtgatc gtgcaaccgc cggttcagtg gtgccttgat
     4801 acggctttcg ccggattgat agcccggcca aatggtctcg cgtgatgtca cctattacag
     4861 caaatcggcc atcggcgcgg tcagtattca aggaatattc cgaacggtca ttcaatccag
     4921 aacggcagac caccattgtc gaggaaattc ggacaatcta ttccggttgc gcaatctagg
     4981 cagttttttg gcccacccat aatgaagatc cacgcgccct ggtggccggg tgcgacaata
     5041 gattatcaat tcgtaggacg gcttacgcga tggcgcgggt cccgcgagaa aatgatgccc
     5101 tcccctggcc gggtccgcgc ttgggaccaa tctgggtaga atgggaaatc attactgcgg
     5161 caacccacat ccatggcgga ttcgccttgt tctaccctac acgttggatt gatcatcttc
     5221 ttcgggcacg catcatgaaa gatatcctag gtccgaaaaa accggggcgc tcgctccatg
     5281 cggaaatctc tgtcgatggc caggattggc aatcggtcat cacgctggcc aaaaatacga
     5341 tcatcaccct ttgtacaacc gcgctgaagt gcggcgatcg gcagttaaga gtgacttcac
     5401 cctggcgaga ggtctgccgt tttgacgacc gacaggtttc ccaccgcgaa tacgaacggc
     5461 gactgtccgg aggaattcgc catcaacgtc agctcacgct ttgccctagg gacaacttct
     5521 tcctctttgc ggagacgttt cttggttcga gggtcacaag ctgggagtat aagttggaaa
     5581 ttgggctttc gcgcaacacg gccgtccacc cgttacccga aacccaagag cttgagcttg
     5641 ccgtggggga ttacgccggt gcgattctgc cgctcgccgt tccggaatgg aaaagcttga
     5701 cgccacaacc cggtctggtt ttcgggggag accacatcgt cgcgcggtac gcgacaaccg
     5761 gccgggcttt tttctttccc ctgtgtttcc tcttttggaa agacaagcga gccgcaggca
     5821 acccgccatc ggcggaaagc tcggcgaacg gtcaacccct cccggtaact tggcgccaac
     5881 tgacggtcag tgagtgcctt cgaaaagtct cggcagatga agccgtgggg tttcgctttc
     5941 aactcgaaaa gcgtcagtgg ctgctgtatc gctccttgac gccacctgca aaccgggccg
     6001 tcctggggca caacctcgtt agcgagttcc tccttgcgcg gtttacgccc aagggacagg
     6061 tcatccccct ggttgaggtc gaactcgatg aagaatgaga ctctactagt ggcagggaaa
     6121 ccggaacgac cacaagctga ttattccccc gtcaccaagg tgccggcctt catcggctga
     6181 tagaggaacg aatcccaata atcgcgatcg actttgtaat agtcgatggg atcattgggt
     6241 ccgaggtctt tgggaatgaa cccaaaccgt tgcatctccc ggatatagtg ctgattggga
     6301 cggaagcccg gcatgtcgaa ccgcttgcct tcctgtagtc gccgatgggc atcttggatg
     6361 gcggccaagg ttttttgata cagcggatcc tgggtatctg cgaagatcgg ttttccgcaa
     6421 agctccaatc cgccagcagc tttggccaag ggtgccaaaa ggatgtacga tttttccgga
     6481 cgatcgagat tactaagcgg ttccgtggca ccgggaaatc cgccgcggaa gatcaaggac
     6541 tttttggggc cttccgccgt ttgaatttcc cccatatggc aatttccgca gcgttgtccc
     6601 agctctcccc aaggcaactg aacgggatag aaaccacatc ccaacgcggc gtatgtgccg
     6661 ggatagcatg cgctagtctc aatccacagg cgaatggtct tgacttccaa aggactgagt
     6721 tttgccccat agtggctgcc gtcgatgagc ttcatcaacc ggctggccgc actgccatag
     6781 ctgtaaggag gccgattccc ccgcggttca ttacggccat ccgagaccag attgtgcacg
     6841 cggatagtcc aatagcttat cgaataccgc gccgtgtgat cacccgtcag gtcgatcttt
     6901 ccttctcggc gatcgggctg gtgacaggcc acacagtgac gatcgagaat gggctggata
     6961 tcacggggaa aatccaggac ttttggcacg tcgctgattg gttgaatctg atgcggggga
     7021 tgacgcaggg ccaacaaatc agtgctgacc gcgtgaggtg cgtgggttcg gacctcatgg
     7081 cagcccacac aactggtcac ttcccctggc tgaagcgtca caaagctgtg catccgtttg
     7141 atggcgatat cgttttcgtc caaggccacg aagaaaagcg attgcaaggc ggggacttca
     7201 aaattggctg acccatctgg ctcgacgggc acttcaccca caatttcagc cagggtgaaa
     7261 gacccgccaa tcgtcaacgg ctccattccg cccgaaaaat tgaccggttt cggcaattgc
     7321 ttgaggacga gaagtttttt gaccgctccg cgcggcacac cttccatgtt tcgcccacgg
     7381 tagatatcct ccagcaccaa agtccctgtg gccttggtta aatccacgcg cggcgggatg
     7441 atcctttccc gggggcgcgg cacgatgggc cttggttcgt gacactgaaa cgtcttttgc
     7501 tcctctggcg gcaattgata aatcagttcg gctgtaccct ccccatccat gacaaacaat
     7561 ccccggggat gggccaccaa aaagcagtcc tcgctgatcg cgtacgggtc tttccagttg
     7621 cgttcctttc ccagaatctt gaccgcatcc tgacgatcgg gtccaaggct gggatcgacc
     7681 acagccacag gccctaggtg ttcgggacga ccgtggcccg gtgaccacga caccaccacc
     7741 ttgttggtgt tgggaatcgg tttggcatcc aacatcgcaa caccgccata catgttgccg
     7801 aaaaagacag tttggaatgt cccatccgga ttcatggtcc acaggtgatg gaaatggacc
     7861 tggctgcggt caacatattc ccaccgcata taaaggacac gaccgtccgg caacatccaa
     7921 ggagtgttgt cgtgatcgtt attgctggaa agcggccgga tgttctttcc gtcagcatcg
     7981 caccggtaaa ggatcgccac tcgggtgtac caacagttga caaatcggtg gcaacgcgaa
     8041 gaacaaaaga tgatacttcc atcgggacaa tacgtgggct cgatgtcatc atctggccca
     8101 tcggtcaact ggcggagacc cgtcccgtca acattaattt cataaaggtg aaaaggatgc
     8161 gtccccccct ttcgatacga gaacagaatc ttttggccat cgtaatggac ttgggg
//
LOCUS       DAOPTR010000023         8141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_7357_length_8141_cov_3.376824, whole genome shotgun sequence.
ACCESSION   DAOPTR010000023 DAOPTR010000000
VERSION     DAOPTR010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 8141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8141
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7357_length_8141_cov_3.376824"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..2050)
                     /locus_tag="PKI05_00925"
     CDS             complement(125..2050)
                     /locus_tag="PKI05_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOA50785.1"
                     /translation="MTCQPVPFFRSSRAILWPALLVTLSLALGRAAFGEEKAPEAAPP
                     QGLRILFLGDNPWNGGTLRRVFEAFAATYRRNIEITEIPAKDRPLGEVIAGLIESTPE
                     KGRFDVVVAFDHPLRQVVQRDRAELITEIDTLEGLAPRIVWILSPYRKPTLRVETERQ
                     KILGRLPLPQGFSTLPLIRLFHQTRKLAGGAAWVNDLSLDRPTSFSDYLTAALLAQVL
                     WGPAPQFPNDIRISDTASLRIPEEALPVLAKVLATLEITLPKGPALANAFAPELSPPQ
                     RFVGNGLPLTLTSGVIPGFFDWNADGRLELVLCGRETGQWEIWTWPTPDKPLSLAQAA
                     RFPAETVNPDLAAMIKAGATVFQVVDFDYDRHPDLLIGTNEGKLALCRGQAGMVFGRP
                     EVCMLEDGKPFQVPYLHRAFLGELTKDGVWDLVLGDGAGQVFLTSLVEQGGRFLWGQP
                     RPVTIEGLTLQVDGEASPVIADWDLDGLPDLIVGCRNGGVLWIRNVGSNEQPAWAPPV
                     SLVWPPEEEETLLIPYRNGMELWTPTPGWSTQPSVADLDGDHLPDLIIGDSNCRVVKY
                     RDFTAEEQKEVDALLKKRREIVEKLSQSPPEEIARQKAALWEITLELIQKSTDRRYER
                     CGWVWYLRRQSLATPDE"
     gene            complement(2143..3135)
                     /locus_tag="PKI05_00930"
     CDS             complement(2143..3135)
                     /locus_tag="PKI05_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOA50786.1"
                     /translation="MKTRIILCRFNQIVVSIPSLMLLLVAMGVGRDGIASESQPGQQV
                     SKKIVFIAGRPSHGFGSHEHNAGCLLLAKKLKEALPNVETVVYTNGWPQQTDALTDAD
                     AIVIFADGGGGNIIAQHLDELQKAIDKGAGLAVLHYALDIAAPEGREKLKSWIGGYYE
                     TYWSVNPHWRAEFKALPEHPITRGVRPFAIDDEWYYHMRFVDDTTRLVPILTAIPPDS
                     TRERPDGPHSGNPTVRSRKGMPEHVAWAYERPDGGRGFGFTGGHHHWNWAHDQFRTLV
                     LNAIAWCARIEIPEQGLRTTTPTIEELLANQDEPPPANFQPETIKRQIESWNQQ"
     gene            complement(3319..4749)
                     /locus_tag="PKI05_00935"
     CDS             complement(3319..4749)
                     /locus_tag="PKI05_00935"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOA50787.1"
                     /translation="MGSGAILNDWDSLWENDIRFPQCHSHEQLARLFPEINVSFVASF
                     NENHAPLAFWCDDHWPEQPAITLKNLRSFLPETMTPTEWRPFAYGEGQNILGFVTRFQ
                     APEECYQMKSPPAGFVVGLYRGTEQAFCAAAPLLIPFARALGLSAWLADRLLLSCTKI
                     EQMEQEKISLKQSHSEAVSMALEEHELVLLAEEKQKVLQELIEAKEAAYRAKSQFLAN
                     ISHELRTPLHAILSYAAFGIKKIETADKPTLLRYFQQIETSGKSLLAFFNDLLDLSKL
                     EAGKMRYERSVSEVVWLIHSVLEEFRSLAEQKRIKLEYIGPQCFRTELDPTRFAQVIR
                     NLISNAVKFTREDGHVWVTLVPQEDHFLLRVDDEGCGIPDGELDSIFESFYQSSRTRT
                     SAGGTGLGLAICREIVLAHGGRIWAENRPEGGARFSVFWPITRTTDHLDSQGNPHAPP
                     ATVVGADDEASSSNTQDVLVTATAEA"
     gene            complement(4893..5717)
                     /locus_tag="PKI05_00940"
     CDS             complement(4893..5717)
                     /locus_tag="PKI05_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOA50788.1"
                     /translation="MSRSWIRVDSLDDPRLAPYRNLPERTLRGESIFITEGWLVTERL
                     LKSTYETESVLLAEDCAAEYADQVPDSIPLYVGPRELLYQVVGYQFHQGVLAVGRRRI
                     LPSVRDVLPPPDRPGPLMVVVCPQITKPENIGLVFRSAAAFDVDAVILGERCAEPFSR
                     RILRVSMGAVLHLPMAKTNNLREELAILKREWNVALVASVLDPTAVSLPDFSPPERVG
                     LLLGHETEGLREGWADLCDYSVTIPMSSLVDSLNLGVAAGIFLYEFRRNGLKRRSA"
     gene            complement(5737..7497)
                     /locus_tag="PKI05_00945"
     CDS             complement(5737..7497)
                     /locus_tag="PKI05_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOA50789.1"
                     /translation="MTTVRCIIRALWVTSCLAWLVLCQGAVKGEESTSIWSAPPESLA
                     KWQAMRFGLFIHWGPVSLKGTEIGWSRGAQVPIEEYDNLYKQFNPTKFNADEWVSIAK
                     EAGMKYLVITSKHHDGFCIWDSEYTDYDIMSTPFKRDILKELSEACRRQGIVFCAYHS
                     ICDWYHPDYPLGSPGGRTKKPNPNMERYVQYLHNQIAEIIRKYGPLGVIWFDGDWEEP
                     WTHEHGLALYKYIRTLQPDILINNRIDKGRRGMKGDTAGPQFVGDFDTPEQRVGAFNR
                     ERPWETCMTICRQWAWKPNDELKTLSQCLEILLRTVGGDGNLLLNVGPMPDGQIEPRQ
                     VERLKEIGAWLKKYGDGVYGTRGGPFKPGPWGASTCKENKIYVFIFNWPKEGPLVLPA
                     INQKIVSAVARTGGDVKVEQSNTQITVSLSPEQRDPLVTVLELTVSGTAFDIPPVAVP
                     ALSGALSVNRPTKASNVFQKQVARFGPQMAVDDDSETRWATDAGTHSAWLEVDLERPC
                     VVDRAVIEEAFAPRVQEFQIECAQGDEWVPCFKGTTIGERGEFRFRAVTGQRFRLNIL
                     KATEGPTIWEFQLFGSEKKE"
     gene            complement(7540..>8141)
                     /locus_tag="PKI05_00950"
     CDS             complement(7540..>8141)
                     /locus_tag="PKI05_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50790.1"
                     /translation="GTTVNHNKIHDVYSFSYGGWGLYTDEGSTHIEMAYNLVYRTKTG
                     SFHQHYGKENRIHNNILVNSVEHQIQRTRTEQHISFFFERNIVAWDNDSPLLGSNWKD
                     NNFRMDYNLYWHGGKPVVFPGNLTLEQWQKERGQDVHSIIADPKFVDPAKDDYRLQPD
                     SPAFSLGFKAFDPNEAGRTSPPQLTANLPEVPTGLAKGE"
BASE COUNT         1766 a         2305 c         2256 g         1814 t
ORIGIN      
        1 ttgcgctgtc aggtcaagtg aacgtcaacc ggtttcactc cctcaccggc ctacgttggc
       61 ggttcggaac gttggcgctt cggaagatgg caatatgatg ccacttggaa cgttggcgct
      121 ggtgttattc atcgggggta gccaacgatt ggcgccgcag gtaccaaacc cagccgcatc
      181 gttcgtagcg tctatcggtt gatttttgaa tcaactcaag cgtgatttcc caaagtgccg
      241 ctttttgtct ggcgatttct tccggtggtg attggctgag tttctcgacg atttccctgc
      301 gtttcttgag taaagcatcg acttcctttt gttcctctgc cgtaaaatct cgatacttga
      361 cgacccggca attggaatcg ccgatgatca aatcgggcaa atgatcgccg tccaggtcgg
      421 ccacggatgg ttgcgttgac catcccggtg tcggcgtcca aagttccatt ccatttcgat
      481 agggaatgag caacgtctct tcttcttccg ggggccagac gaggctgacc ggtggtgccc
      541 aggcgggctg ctcatttgaa ccgacgttcc gaatccaaag gacgcctccg tttcggcagc
      601 cgacaatcaa atcgggcagg ccgtcaagat cccaatcggc gatcacgggc gaggcctcac
      661 catcgacttg cagggtaagt ccttcaatgg taacggggcg cggctggccc cagagaaaac
      721 gacctccctg ctccaccagg gatgtcagga acacctgccc tgccccatca ccgagcacga
      781 ggtcccagac accatctttg gttagttcac cgagaaatgc ccgatgtaaa taggggacct
      841 gaaacggctt gccgtcttcc agcatgcaca cttctggccg cccaaatacc atacccgctt
      901 ggccgcggca gagggcaagc ttgccctcat ttgttccgat cagcaaatcg gggtggcgat
      961 cataatcgaa atccaccact tgaaagacag ttgccccagc cttgatcatc gccgccaagt
     1021 ccgggttcac ggtttcggcg ggaaatcgcg cagcctgggc caacgacaac ggcttgtcgg
     1081 gagttggcca cgtccagatt tcccattggc ccgtttcccg tccacacagc acaagctcca
     1141 atcgcccatc ggcgttccaa tcaaaaaaac caggaattac gccagaggtc aacgtcagag
     1201 gtaggccgtt gccgacaaac cgctggggcg gactcagttc cggggcgaac gcattggcaa
     1261 gggccgggcc tttggggaga gttatttcca gagtggcaag taccttggcg agcacgggca
     1321 gcgcctcttc cgggatgcgt aggctggccg tatcgctgat gcgaatgtcg ttcggaaatt
     1381 gaggagcggg accccaaagg acctgggcaa ggagggcggc cgtgagatag tcgctgaagc
     1441 tcgtggggcg atccaaactt aggtcgttga cccaggccgc cccaccggcc aacttccgag
     1501 tttgatggaa cagccggatc agcggtaagg tagagaatcc ttgaggcagg ggcaatcgcc
     1561 cgaggatttt ttgccgctcg gtctccacac ggagtgtcgg ttttcggtac ggggaaagga
     1621 tccagacgat tctcggggcc agtccctcga gcgtatcgat ctccgtgatc agttcggccc
     1681 gatcccgctg gaccacctgc cgcaggggat ggtcaaacgc cacaacgacg tcgaagcgtc
     1741 ccttttccgg cgtgctctca attagaccgg cgatgacctc gcccaggggg cggtctttcg
     1801 cagggatttc cgtgatctcg atgtttcgcc tgtaggtcgc ggcaaaggcc tcaaacacgc
     1861 ggcgaagcgt gccgccattc catgggttat ctccaagaaa gaggatgcga agaccctgcg
     1921 gcggtgcggc ttcgggggcc ttctcttcgc cgaaagcggc cctgcccagg gccaagctca
     1981 aggtcaccag caaggccggc cacaaaatcg cacggctgga gcggaagaaa ggtacaggtt
     2041 gacaggtcat tctcggagca gctcaacgtc ctcgactgcg gcacatgggc cgacgtttcg
     2101 ttttctcacg cggttaggtc tctcttcgcc gtgagacgat gactattgct gattccaact
     2161 ctcgatctgt cttttgatcg tttccggctg gaaattcgcc gggggcggct catcctgatt
     2221 ggccagtaac tcctctatcg tgggagtcgt cgtgcgcagg ccctgctctg ggatttcgat
     2281 tcgggcacac caggcgatgg cattcaggac gagcgtccgg aactgatcgt gcgcccaatt
     2341 ccaatggtga tgccctccgg taaaaccaaa accgcggccg ccgtcaggcc gttcataagc
     2401 ccacgccacg tgctctggca tgccttttcg agagcgaacc gtcggattgc cgctgtgggg
     2461 gccgtcgggt ctttctcgcg tgctgtcggg cgggatggcc gtcaaaatgg gcacaagtct
     2521 tgtcgtgtca tcgacgaacc gcatatggta gtaccattcg tcgtcaatgg cgaaggggcg
     2581 gacaccgcgg gtgatggggt gctcgggcaa agccttgaac tcagctcgcc agtgcggatt
     2641 gaccgaccag tacgtttcat aatagccacc gatccaggat tttagttttt cgcgtccctc
     2701 gggcgcagca atatccaggg cgtagtgtaa aaccgctaga ccagctcctt tgtcaatcgc
     2761 cttctgaagt tcgtccaggt gttgtgcgat gatgttgcca ccaccaccgt cggcaaagat
     2821 gacaatcgca tccgcgtccg tcagggcatc cgtctgctgg ggccaaccgt tggtgtaaac
     2881 aaccgtttcg acgttgggca aggcttcctt cagcttcttg gccaacaaaa gacatcctgc
     2941 gttatgctca tggctgccga agccgtgact cggtcggccc gcaataaaca cgatcttttt
     3001 ggaaacttgc tgaccgggct ggctttcgga ggcgataccg tctctgccaa cgcccattgc
     3061 caccaaaagg agcatcaacg agggaatcga taccacgatt tgattaaagc gacagagaat
     3121 tatgcgagtt ttcatgggca aggcctccca catatgggca aactgattac acaaacaatc
     3181 gacagcacgc gagcgattcg ctttcaagta caatctgccg acaacaacat tttagttgat
     3241 caggggctat tttgcgcgag aaccctgctc gtttcaatgg aacgtgcacc cctttcacga
     3301 atttgctgcg gcgccgggct acgcctcagc ggtggcggtg acgagaacgt cttgcgtatt
     3361 tgaactcgac gcttcatcgt ccgcgcctac gacagttgcg ggaggagcgt gggggttgcc
     3421 ttgtgaatcc aagtggtctg ttgtccgagt tattggccag aaaaccgaga atcgcgcgcc
     3481 accttcgggt cggttttccg cccagatgcg gcctccatga gccaagacga tttcccggca
     3541 aatcgccaat cctagccctg ttcccccggc cgatgttctt gttctcgaag attggtaaaa
     3601 actctcgaag atgctgtcca gttcaccatc ggggattccg cacccttcgt catcgacgcg
     3661 gaggaggaaa tggtcctcct ggggaacgag ggttacccag acatgtccgt cctctcgtgt
     3721 aaacttcaca gcattgctga tgaggttcct aatcacctgg gcaaatcgtg tggggtccaa
     3781 ttcggtgcga aagcactgcg gcccaatgta ttccagtttg atacgttttt gctcggccag
     3841 ggaacggaac tcttccaaga cgctgtggat gagccagaca acctcggaga ccgatcgctc
     3901 gtaacgcatc ttgccggcct ccagcttgga gagatcgaga agatcattga aaaacgcaag
     3961 caaggatttt ccgcttgtct cgatttgctg aaaataccga agaagggtgg gcttgtccgc
     4021 ggtttcgatc ttctttatgc caaatgcagc ataactgaga atggcgtgca gaggcgtcct
     4081 caattcgtgg gagatattgg ctaaaaactg gctctttgcc cgataggcgg cttccttggc
     4141 ctcgatgagt tcttgcaaga ccttttgttt ttcttcggcc agaagaacca attcgtgctc
     4201 ctcaagagcc atgctcacgg cttctgaatg tgactgctta agcgagattt tctcctgctc
     4261 catttgttcg attttcgtac aactcaatag caaacgatct gccagccacg ctgataggcc
     4321 cagcgcgcgg gcaaaaggaa tcaagagcgg cgctgctgca cagaaagcct gctcggttcc
     4381 gcgatacaat cccacgacga aacccgccgg tggagatttc atctggtagc attcttctgg
     4441 cgcttgaaac cgagtgacga atccgaggat attctggcct tcaccgtagg caaacggcct
     4501 ccactcagtt ggggtcatcg tctctggcag gaatgaacgg agattcttga gggtgatcgc
     4561 cggctgttct ggccagtgat cgtcacacca gaacgcaagc ggcgcatggt tctcgttgaa
     4621 agatgccaca aaacttacgt tgatttcggg aaaaagacgc gcgagctgct catgagagtg
     4681 gcactgggga aaacggatat cgttttccca caatgaatcc cagtcgttca ggatggcgcc
     4741 gctgcccatc tttcgtcttt cggatttccg atcgtggtta agatttgtga aatggcacac
     4801 cttaggcgcg tacgcaccta tcgcctttgg gggcgtccag aaaagcttaa ctcaaaaagt
     4861 aggacacagc cgtccggcac caggggaatc cctcaagccg atcgccgttt taggccgttt
     4921 cttcggaatt catagagaaa aatgcccgct gccaccccta agttgaggga gtccaccaga
     4981 ctagacatgg gaatggtcac ggagtaatcg cacagatcgg cccacccttc ccgaagtcct
     5041 tcggtttcat gccccaatag caagccaact ctttcggggg gcgaaaagtc tggcaatgaa
     5101 actgccgtgg ggtccagtac actggccacc aaggccacat tccattcccg cttgaggatc
     5161 gccaattctt cccggagatt gttggtcttt gccattggca ggtgaagcac cgcgcccata
     5221 gaaacgcgga ggatgcgtcg ggaaaacggt tcagcgcacc gctcacctaa aatgacggca
     5281 tctacgtcga acgcggccgc ggagcgaaac accaggccga tgttctcggg ctttgtgatc
     5341 tgtggacaaa cgaccaccat gagggggccc ggtcgatcag ggggcggtag aacgtcccgc
     5401 acggacggca agatacgtcg tcggcccacg gcgaggacgc cctgatgaaa ctggtagccc
     5461 accacttgat aaaggagttc ccgcggtcca acgtaaagcg gaattgaatc gggaacttga
     5521 tcggcatatt cggccgcgca atcctcggcc agcaagacgg actccgtttc gtaggtgctt
     5581 ttcaacaagc gctccgtgac gagccaaccc tctgtgatga agattgactc gccacggagc
     5641 gtgcgttccg ggagattccg atatggggca agccgaggat cgtccagcga atcaacgcgg
     5701 atccacgagc gggacataaa tcggcaaaag acctctttat tctttcttct cgctcccgaa
     5761 aagctggaac tcccagatag tcgggccttc cgttgccttg aggatgttca accgaaaccg
     5821 ttgccctgtc acagcccgga agcgaaactc accgcgctcg ccgatggtcg tccctttgaa
     5881 gcacgggacc cactcgtcgc cttgggcaca ctcaatttga aactcctgga cgcgaggggc
     5941 gaaggcctct tcgatgacgg ctcgatccac aacgcacggc cgttccaggt caacctcgag
     6001 ccaagcgcta tgcgttccgg catcggtggc ccaacgggtt tcggagtcat catccacggc
     6061 catttgcgga ccaaaccgcg cgacctgctt ctgaaagaca ttggaggcct tggtgggcct
     6121 gttcacactg agggcaccac tgagtgctgg cacggccacc ggcgggatat cgaaggctgt
     6181 gccgctgacg gttagctcca agactgtcac caaggggtcg cgctgctcgg gcgagagact
     6241 caccgtgatc tgcgtgttgc tttgttcgac cttcacatct ccgccggttc tggcaaccgc
     6301 agagacaatc ttctgattga tggccggaag aacaagcggt ccctctttag gccagttaaa
     6361 gatgaacacg taaatcttgt tctctttaca ggtggaagcc ccccagggac ctggtttgaa
     6421 gggaccaccc cgagttccat aaactccgtc gccgtatttc ttcagccagg caccgatctc
     6481 cttgaggcgt tccacctgac gcggttcgat ttgcccatcc ggcatagggc cgacatttaa
     6541 aaggagattc ccatctccac ccaccgtgcg gaggagtatc tccaagcatt gcgagagcgt
     6601 tttcaactca tcgttgggct tccaagccca ttggcggcag atggtcatac aggtttccca
     6661 ggggcgctcc cgattgaagg ccccgacgcg ttgttccggt gtatcaaaat cgcccacaaa
     6721 ctgtggccct gcggtgtcgc ctttcatccc ccggcgacct ttgtcgatac ggttgttgat
     6781 gaggatatcc ggctgcaatg tgcggatgta cttataaagt gccaggccgt gttcatgggt
     6841 ccatggctct tcccaatcgc catcgaacca aatgacgccc agcggaccgt acttgcgaat
     6901 aatttcggct atttgattgt gcaaatactg aacataccgc tccatattgg gattgggctt
     6961 tttcgtccgg ccaccaggtg agcccaacgg ataatccggg tgataccagt cgcagatgga
     7021 gtggtaggcg caaaagacga tcccctgacg acggcaggcc tccgacagtt ccttcaaaat
     7081 gtcccgctta aaaggcgtgg acatgatgtc gtagtcggta tattccgagt cccaaatgca
     7141 aaaaccatcg tgatgcttgg aagtgatcac gagatacttc atgccggcct ccttggcgat
     7201 ggagacccac tcgtcggcat taaacttcgt ggggttaaat tgtttgtaca ggttgtcata
     7261 ttcctcgatg gggacctgag caccgcgtga ccaaccgatt tccgtgccct taagactgac
     7321 agggccccag tgaataaaca acccaaagcg catggcctgc catttggcga gcgactccgg
     7381 aggagcgctc cagatcgagg tcgattcctc ccccttcacc gctccctgac aaagcactag
     7441 ccaagcgaga caactggtta cccaaagggc acgaatgatg cagcgcaccg tggtcatact
     7501 cagaccctcc aaaaatgtgt tattgtaaac gacagcgttt tactctccct tggcaagacc
     7561 cgtgggaacc tccggcagat tcgccgtcag ttgcggcggg ctggtccgac ctgcctcgtt
     7621 tgggtcaaag gccttgaaac ccagcgagaa ggccggtgaa tccggctgga gccggtaatc
     7681 atccttggcc ggatcaacga atttcggatc ggcaatgatc gagtgcacgt cctgaccgcg
     7741 ttctttttgc cattgttcca gcgtcaagtt gccagggaaa acgaccggct tgccaccgtg
     7801 ccaataaagg ttgtaatcca tgcggaaatt gttgtccttc caattgcttc ccaacagcgg
     7861 gctatcattg tcccatgcaa cgatatttcg ctcgaagaag aacgaaatgt gttgttcggt
     7921 gcgggtccgc tggatttgat gctcgaccga gttgacaagg atgttgttat ggatacggtt
     7981 ctccttcccg taatgttggt gaaagctgcc cgtctttgtg cgatacacca agttgtacgc
     8041 catttcgatg tgcgtggaac cctcatccgt gtacagtccc cagccaccgt agctaaacga
     8101 gtacacatcg tgaatcttgt tgtgattgac ggtcgtgcct g
//
LOCUS       DAOPTR010000024         8041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_7462_length_8041_cov_3.828951, whole genome shotgun sequence.
ACCESSION   DAOPTR010000024 DAOPTR010000000
VERSION     DAOPTR010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 8041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8041
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7462_length_8041_cov_3.828951"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..752
                     /locus_tag="PKI05_00955"
     CDS             150..752
                     /locus_tag="PKI05_00955"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOA50791.1"
                     /translation="MLYVDGFNLYFGLRTKGWKRYYWLDVQRLGEKLLRPNQSLVGVK
                     YFTADIRGPADKHRRQQTYLDALSTYRPNLTIMRGHYLIKKKQCRCCACEFEMPEEKK
                     TDVNISTQMLVDAFLDRFDVAILISGDSDLVPPIEAIRQYQPQKRVVVAFPPARTSED
                     LKRAAHAWFRINEQKLRLSLLPNPVVKPNGHNLYKPTEWK"
     gene            complement(842..1039)
                     /locus_tag="PKI05_00960"
     CDS             complement(842..1039)
                     /locus_tag="PKI05_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50792.1"
                     /translation="MPFHGLLSGGATLVCDRYDSPLNLYEQRATIPPSAPMLQVQQIV
                     HHQHAHHHRKYRHNNKYRHKN"
     gene            complement(1724..4111)
                     /locus_tag="PKI05_00965"
     CDS             complement(1724..4111)
                     /locus_tag="PKI05_00965"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOA50793.1"
                     /translation="MSGPQTSQPFDPYYTWLGIPPSERPISYYRLLGLKPFESNETAI
                     EAAVDQRLAYLRTKALGPHAAFAEKLISEITQARLVLLDPVRKQAYDAALSSAAHRGP
                     VPSQQEAPARHSGTPRVAAPLGTGSYMPGNVRRFRRKSRLTKDQWIAALGLGLGLCFV
                     IGVLVLWLSGKKIGSQGDRNPSPRQEQLAGGPPAPRVNDGSPADAGARLHGLPQPGRI
                     PGANPPESLPRQAPPAPPPAPDAEAKPAVNPMANPAENNRWLEKVRRATVVIQGDRGQ
                     GSGFFVTTGAGKPVVVTNAHVIRDNFNLRLKLFDGREIPVQQGAVLADRDLAILAVEA
                     INLPQPLELRGELPQVAEQVFAYGAPQGLTGTLTAGIVSAVRSSSGIRDVVDDGSFDK
                     PVRKAEVRWIQTTAPISPGNSGGPLLDQQGRVVGINSLAFNNLLGQNLNFALSSTEVL
                     PHLAAPQFAALPKSAPIGPGVFGVNQPQGGTPKSSPNSLYPFEQYSCQDITLPSGAVF
                     QPGKVRPPEKWRLAIESGHVLVCRRGQITVSAKKEVGGGLFREDVSFGVGAVGEEGAA
                     YIGGSRTRYHYTPGVVSPFAFYVFYDDNDVMRAAMAFEKGLLNGPAVLFDENGHPYVI
                     ANYYRGTRTGPLVVYDEEQWPAVYVEFTRNRKHGLVCVFDQHLPSWIENWRANQLVEA
                     CLIKWEGNQCQVLLASALTNPDDQALSQERMAKVSRYLDELDSLESELKRNVAEAFRW
                     ADNEIKKRRAAQFSAAATAAILARHRAREAAGAALRAEANARFQTGSGPPPLPIP"
     gene            4445..5293
                     /locus_tag="PKI05_00970"
     CDS             4445..5293
                     /locus_tag="PKI05_00970"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOA50794.1"
                     /translation="MQTPDDGIEITRVLAETFLVDGEYHQTIDSTNNRAKALAGSGQI
                     GLPYLVLAEEQTAGRGRALRRWWTGRGSLAFSIILPLTEERPVNRLSEKENSTSRARP
                     KAFLCEDGIRLLGLAGALAVYRAASRHLPSDISLGIHWPNDVYANDRKLAGVLVEITG
                     NQRAVVGIGVNVNNRSEDAPPHVQHKVATLRELCGQPQNRTELLIDILQEFEPLFELL
                     KNQPERLAEEANRVCLQKGRELVLEVEQGLVTGLCLGIDPTGALVLKTVSGERRFLSG
                     TVVAVE"
     gene            5436..6827
                     /locus_tag="PKI05_00975"
     CDS             5436..6827
                     /locus_tag="PKI05_00975"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOA50795.1"
                     /translation="MNQGLVAREMLDWEFLESYLRQRDAHFAVHWEWITEVRHKLEAN
                     AGLDRGAVTDDVARMSERIERLRYQLLQERSDPDAADYQAAEVLDSLFAILTDRETTT
                     PRHLQQLLEDDTFAVEAIKSLEWCLNPDVPLDTVIASARRLTKEHLGANLPDQGPESA
                     SWPIFLYAPLYLSSYCVNHCLYCGFRFPETTPRRLLSADEAVEQVGLLVGRGMRHILL
                     VAGDYPQKTDIDYYVETITAIRRRWPVSLAVEIAPQSTAGYRSLVKAGVIGVTLYQET
                     YDRSLYRTYHIRGPKAHYAWRLEGLERAAEAGMRRLGLGILLGLGDPVSDARAMVRHA
                     YYLKERFPDRRIAFSLPRIHEGPEGFQIPNPVNDETLIRLYCGLRHAMPGAELVLSTR
                     EMPQLRDLLAATCITQLSAGSSTVPGGYDEMGTHFGDEHDGQFRVVDRRSVNDVIAVL
                     KSQGHAIIWQPRE"
     gene            7156..7905
                     /locus_tag="PKI05_00980"
     CDS             7156..7905
                     /locus_tag="PKI05_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007340410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOA50796.1"
                     /translation="MFVAATTRCFANLPLEAAFERLNDLEFGYVEIMLHESDGHLKPS
                     EVAADLDRAIALCRKTYRLTPIAYSVDIEAQGDEYYRQFAACCKLAKATRVVTVVVRA
                     SELGTPFNEEVERLQNLVAIATMEEVVVALLTERGRMSELPETAAVLCHHAKGLGICL
                     DPSHLIYGRETPLNPEPALKYVRHVRLRDTRKDKFQVLVGQGKVEYGKLLANLGRYEY
                     NRALAIDIAPEPGVDQSSELRKLRLLLETLL"
BASE COUNT         1800 a         2194 c         2278 g         1769 t
ORIGIN      
        1 gggttcacag tcagggtcat ttaaagataa tattatggct acttcggggt gtcaagctgt
       61 caagttcgaa ctgccctgga tgcgcaaagt tggcaggggg ggtatcttgc cctatttggg
      121 ggtgtcgcgt ggcaaatgac gtgagagtaa tgctttacgt cgatgggttc aacctatact
      181 tcggtctgag aaccaaaggg tggaaacgtt attactggct ggatgtccag cgtctgggag
      241 agaagctgct acgacccaac cagagtttgg taggtgtcaa gtatttcacg gccgacatcc
      301 gtgggccggc tgataagcat cgccgtcagc agacgtatct ggatgcgttg agcacctaca
      361 gaccgaatct gactatcatg cgtggtcatt atctgatcaa gaaaaagcaa tgccggtgtt
      421 gtgcatgcga gttcgagatg ccggaagaga agaagaccga tgtgaacatt tcgacgcaga
      481 tgctggtgga tgctttcctg gaccgtttcg atgtggcaat tttgatttcc ggcgatagtg
      541 acctcgtgcc cccgattgag gcaattcggc agtatcagcc gcaaaagcgt gtggtggtag
      601 cgtttccgcc ggcgcgcact tccgaggacc tgaaacgcgc cgcacatgcg tggttccgga
      661 ttaacgagca aaaactcagg ctctcgctcc tgcccaatcc cgtggtcaaa ccaaacggac
      721 acaatctgta taagccgaca gagtggaagt aatagccaag gcggaccgca ccgttagcca
      781 ccaaatccgt aacgggaatc accgtgaccc gacggcgggg aactcgaaat caaaggcttc
      841 actaattttt gtgacgatac ttgttattgt gacgatactt gcgatgatga tgggcatgct
      901 ggtgatgcac gatctgctgc acctgcaaca tgggagcgct cggcgggatt gtggcacgtt
      961 gctcatagag gttcaacggc gaatcgtaac gatcgcaaac taatgtggca cccccggaaa
     1021 gtaatccgtg gaacggcacg acttacctca accattgttg tcgtgttaaa catggtctgg
     1081 gttggacaca ttttttgcga agtggtcatc cgtttttatt gtcccgtgtt gaagtggcag
     1141 ggcctgtact ggattggagc gctagcagtg attgaccaaa attaggggag caacacgggt
     1201 cgccccaagt taggagaacc acggggttgc gaggagctgg gcaaccacag ggggttgccc
     1261 ctacacacgc aattaaaagc cgtaggggca ggcccccgtg cctgcccatt ctctttctct
     1321 tgcctgcgcg aaaccaacgg gctccgacgc tcttcggcaa gttaagctcg gcgaaatctt
     1381 gtcaagcctt ttctggccct gtttatttcc cgaccctgcc cgaagcatcc ggccgctcgg
     1441 gtagactttc tttcgcgcgc tttcggggga aagcaacttt ggggaatcac gtcagatatg
     1501 accatagaac gccgaggacg gcgtccctcc caacgccaaa cgcgcggagg gcgccccttc
     1561 caacttgtca accgatacga cgaacagtcg gtagcacacg gcgggtccgg cagagtgagt
     1621 gcctacaaaa tgccggtcgc cggggatggc gtcccttccc aaaaccgcgg cgctagcggg
     1681 ttacgctccg tcgtggccgg atcaatcgca gccccattgg ttactagggt atcggaagcg
     1741 gcggcggacc cgaacccgtc tggaaacggg cgttggcctc ggcacgaagc gcagcaccgg
     1801 cggcttctcg ggcgcgatgt cgggcaagga tcgctgctgt ggctgccgca gagaactgtg
     1861 ctgcccgacg tttcttgatt tcattgtccg cccaacggaa ggcctcggcc acattgcgtt
     1921 ttaattcgga ctctagactg tccaattcgt caagataccg cgacacttta gccatccgct
     1981 cttgcgataa tgcttgatcg tccggatttg tcagcgccga tgccagcaaa acctgacact
     2041 ggtttccttc ccacttgatc aagcaagcct ccaccagttg gttggccctc caattctcga
     2101 tccaggaagg caaatgttga tcgaagacac acaccaaccc atgtttgcgg tttcgtgtga
     2161 attcaacata caccgccggc cactgttcct catcatagac aacaaggggt cccgtccgcg
     2221 ttccacggta gtagttcgca atcacataag gatgcccgtt ttcatcgaag aggaccgctg
     2281 gtccgttgag caatcctttt tcgaaggcca tggctgcccg catcacatcg ttatcgtcgt
     2341 aaaacacgta aaaagcaaaa ggtgacacga ccccgggcgt gtaatggtac cgtgttctgc
     2401 ttcctccgat gtaagccgca ccttcctcac cgacagcacc aactccaaaa ctaacgtctt
     2461 cccgaaacag accaccaccg acctcttttt tcgccgagac ggtgatctgt ccgcgacggc
     2521 acacgagaac atgtccagac tcgatggcca atcgccattt ctccggggga cgcaccttcc
     2581 ccggttgaaa gaccgcccct gagggtagcg tgatgtcctg acacgaatat tgctcgaatg
     2641 ggtacaacga attcggactc gatttcggcg ttcctccttg tggttgatta acaccaaaaa
     2701 cacccggccc aatgggtgcg ctttttggta aggccgcaaa ctgcggggcc gccagatgag
     2761 gcagcacctc agtcgaggaa agggcgaaat tcaagttctg cccgagaagg ttattgaatg
     2821 ccaaggagtt gatcccaacg acccgcccct gttggtccaa aagtggacca ccactattgc
     2881 ccggggaaat tggagccgtc gtttgaatcc aacggacctc tgctttgcga acgggtttgt
     2941 cgaaacttcc atcatcgacc acgtcccgaa ttccactgct tgatcgcacg gcactgacga
     3001 tgcccgccgt gagcgttccg gtcaaaccct gcggtgcacc ataggcgaag acctgctccg
     3061 caacctgcgg aagttccccc ctgagctcga ggggctgagg caaattgatg gcctcgacgg
     3121 caagaattgc caaatcccga tcagccaaaa cagccccctg ctgaacggga atctctcgtc
     3181 catcgaaaag cttgagccta aggttgaagt tgtcccggat gacatgggca ttcgtaacga
     3241 ccaccggttt tcccgcaccc gtggtgacaa agaagccgct cccttgccct cgatcccctt
     3301 ggatgacgac ggtagcgcgt ctgacttttt ccagccaacg gttattctcg gccggattgg
     3361 ccatcgggtt gacagccggc tttgcttctg catcgggggc aggaggtggc gcgggcggcg
     3421 cctgacgcgg taaagattct gggggattcg ctccaggtat tcgcccaggt tgcggaagac
     3481 cgtgaagcct agcaccggca tccgcggggg aaccatcgtt cacccgtgga gcaggtggcc
     3541 caccagcgag ctgttcctgc ctcggagaag ggtttcgatc tccctgagaa ccgatctttt
     3601 tgccagaaag ccataatacc agaacaccta tcacaaagca cagtcccaag ccgagaccca
     3661 acgccgcaat ccactggtct ttcgtcaatc ggctttttct gcggaaacgg cggacattcc
     3721 ccggcatgta agaccccgtg ccaagaggtg ccgcgacccg cggcgttcct gagtgtctgg
     3781 ccggtgcttc ttgctgggag ggaacaggcc ctctgtgtgc ggcactcgac aaggcagcat
     3841 cgtaggcctg ttttctcacg gggtcgagca ggacaagcct cgcttgagtg atctcgctta
     3901 tgagcttctc agcgaacgca gcatggggac cgagggcctt ggtgcgcaga taggccagtc
     3961 gctggtccac cgccgcttca atggccgtct cattcgattc gaacggcttc aggccaagga
     4021 ggcggtaata cgaaatcggc cgttcggaag gtgggattcc taaccacgtg taatacgggt
     4081 caaacggttg agacgtctga ggaccgctca ttgtggtgtc ccccgttcag aataagaatg
     4141 atggtttggc ttacctactg cccattatcc gcgccaggtt ggacaaacca tgtccaatct
     4201 ggaaaacaat atcacgaaaa tagcccttaa caacggagaa ttttggcgtg ctttggcctc
     4261 tatatcaagc actgactgag gtttatcccc cgttgggatt agtacaaaaa acaggtataa
     4321 ttggctattc tgtcaacccc acggttggtt gttacaactc gtgttgtcaa caacacttat
     4381 ttccctaccc ttgccgtttg ggcaacgctt ggaatagtcc ccgttcttct ggttagacat
     4441 cacgatgcaa acacctgatg atgggattga aatcacgcgg gttttggccg aaacattcct
     4501 ggtcgatggc gagtatcacc aaacgattga ctcgactaac aatcgggcaa aagcgctggc
     4561 cggttccggt cagatcggct taccatatct cgtgctggcc gaagagcaaa cagcaggccg
     4621 gggacgcgct ctgagacggt ggtggaccgg cagaggaagt ttggcattca gcatcatcct
     4681 tcccctcact gaggaacggc cggtcaatcg gttaagcgaa aaagaaaaca gtacttcacg
     4741 ggcgcggccg aaggctttcc tttgcgaaga tgggattcgc ctcctcggtt tagccggggc
     4801 cttggctgtt taccgagcgg caagtcggca ccttccctcc gacatttctt tggggattca
     4861 ctggccgaat gacgtttacg cgaacgacag aaaacttgcg ggagttttgg tggaaataac
     4921 gggcaaccag cgagccgttg tcggcatcgg cgtcaatgtc aacaatcgaa gcgaggatgc
     4981 cccgcctcac gtccaacata aggttgccac gcttcgggaa ttgtgtggcc agccacaaaa
     5041 tcgaacggag cttcttatcg acattttgca ggagttcgag cctctctttg agctgctcaa
     5101 gaaccaaccc gaaagactgg ccgaggaggc caaccgtgta tgtttgcaaa aggggcgtga
     5161 gctagtcctg gaagtggagc agggcttggt gacggggctc tgcctgggca tcgacccgac
     5221 gggtgcctta gttctcaaga ccgtctcggg tgaacggcga tttttgagcg gcactgtggt
     5281 cgcggtggaa tgaagcggcc ctgaccccat agaattaatg gttgggccca ttgcaccgtg
     5341 caacgcttga tggcctaatc gctcgtgtta atttcacctc cgagcgccgc tttctttatt
     5401 cggaagttag gtttattgcc agggtttttg gtgagatgaa tcagggttta gtagctcgcg
     5461 agatgctaga ttgggagttc ctcgagtcct accttcggca gagggacgcc cattttgcgg
     5521 ttcattggga gtggattaca gaagtccgac ataaattgga ggcgaatgcc ggcctagacc
     5581 gcggcgcggt gactgacgat gtcgcccgaa tgtccgaacg catcgaacgc ctgcgttatc
     5641 aattgttgca ggaacgaagc gacccagatg ccgcagacta ccaagcggct gaggtgctgg
     5701 attcgctgtt tgccattttg acagaccgcg agacaacaac tcctcgtcat ctgcagcagc
     5761 tcttggaaga tgacaccttt gccgtcgagg caatcaaaag cctggagtgg tgcttgaacc
     5821 ccgacgtacc gttggacaca gtcatcgcgt cagcccggcg attgaccaag gaacatctcg
     5881 gcgcaaactt gcccgaccaa ggacccgagt cagcatcttg gccgatcttt ctttatgccc
     5941 cgctttatct ctcaagctac tgtgtgaacc attgcctcta ttgcggattt cgttttccgg
     6001 agacgactcc tcgccgcttg ctctcggccg acgaggcagt ggaacaggtt ggcctattag
     6061 ttggccgggg aatgcggcac attttgcttg tcgcgggcga ttacccacag aaaactgaca
     6121 tcgactacta cgtggagaca atcaccgcga tacgccggcg ctggcccgtc agcttggccg
     6181 tggaaatcgc cccgcaatcg acggcgggct acagaagcct ggtaaaagct ggggtgatcg
     6241 gcgtgacgct ttaccaggaa acttacgacc gaagccttta tcgtacctat catatccgcg
     6301 gaccaaaggc acactacgcc tggcgattag aaggcctaga acgggccgct gaggccggga
     6361 tgcgtcgttt ggggcttgga atcttgctgg ggttggggga tccggtctcc gatgcgcggg
     6421 cgatggtgcg acacgcgtat taccttaaag aacggttccc tgatcggcga atcgccttca
     6481 gtttacctag gattcatgaa gggcccgagg gtttccagat tcccaacccg gtcaatgatg
     6541 aaacgctcat ccgattgtat tgcggactgc gtcacgccat gccgggcgcg gagctggtgc
     6601 tttccacccg cgagatgccg caacttcgtg atctcctcgc cgcaacgtgt attactcaat
     6661 tgagcgcggg cagctctacc gttccagggg gatacgacga aatgggaacg cactttgggg
     6721 acgagcacga cgggcagttc cgcgtggtcg atcggcgctc ggtcaacgat gtcatcgcag
     6781 tgctgaaatc ccaagggcat gccatcattt ggcagccccg ggaataaaaa ggcgaaataa
     6841 gcgaatctga ataagccggg aaatctcggc gaacaaagct cgcaatcagc gaggttgcca
     6901 aatggcctat cgacactgta agctaactac ctaagagtat tgcgtaacag agaacaacat
     6961 tgccccacct tcccgagatt tcggcatgaa aacaacagac ctgttgttgc ccggctatat
     7021 ggcgctgtag cacttattgc tagagtgcgt cgtttgcgca gcgggtcgtg ctacacttaa
     7081 agacgggtgg tctgccacca ggaacggtgg gtaggagtca tcgaatcacg gaatcggact
     7141 aaaaggagtg aaggagtgtt tgttgccgca acaacgcgtt gctttgccaa tcttccatta
     7201 gaagcggctt tcgagcgact caatgacctg gagtttggat acgtcgagat catgctccac
     7261 gagtccgacg gacacctcaa gccgtcggaa gtggccgcag atttagatcg ggctattgct
     7321 ctttgccgga agacgtatcg gctgacgccg attgcttaca gtgttgatat cgaagcccaa
     7381 ggagacgagt attaccggca gtttgccgcc tgctgtaagt tggccaaggc cacccgcgtt
     7441 gtcacggtcg tggtacgtgc ttccgaatta gggacacctt tcaacgagga ggttgaacgc
     7501 cttcagaatc ttgtcgccat cgccaccatg gaagaggtcg tcgtggcatt gttgaccgag
     7561 cgtggccgca tgagcgaatt gcccgaaacg gcggccgtct tgtgtcatca cgccaaaggt
     7621 ttgggaattt gcctcgaccc cagccatctt atctacggcc gagagacgcc gctcaacccc
     7681 gaaccggccc tcaagtacgt tcgacacgtc aggctgcggg atacgagaaa agataagttc
     7741 caagttctgg tgggccaggg taaagtggaa tacgggaagc tcttggccaa tctggggcga
     7801 tatgaatata accgggcctt ggccatcgac atcgcgccgg aacctggcgt ggatcagagc
     7861 tccgaacttc gcaaactgcg gctccttttg gagacgttat tatgacgggc cgcgcaaaga
     7921 accgacgttt ctacccggaa tgagtgcgat gatcgttcca gggggtgttt cgacggtctt
     7981 ttcccttgag acgggtcgaa cgggctagat gccagggatc gcatctttcc cacgcagggc
     8041 g
//
LOCUS       DAOPTR010000025         8035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_7469_length_8035_cov_3.833459, whole genome shotgun sequence.
ACCESSION   DAOPTR010000025 DAOPTR010000000
VERSION     DAOPTR010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 8035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8035
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7469_length_8035_cov_3.833459"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1423
                     /locus_tag="PKI05_00985"
     CDS             <1..1423
                     /locus_tag="PKI05_00985"
                     /inference="COORDINATES: protein motif:HMM:NF027224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BNR repeat-containing protein"
                     /protein_id="HOA50797.1"
                     /translation="RIRFFLLFTPLIIGGCWWGVNMQDATAAESAWVLRAAVPLAEKT
                     WRWNWASWDQEKIVTFGEYQYTVFWDADGVFVLARRHLADNTVQLVRMPGWRLSVDDA
                     HRNTCLGISEKDGRLHFSWDHHNNPLNYGRTRAGFLTQPPAQISAGDFEPRQPPAEGD
                     FPMSRVTYPRFINDNKGGLYLFYREGGSGNGDNYLWRYDAETGRWTMVGCVFSKRGTY
                     APWNNSTSRNAYFHDLLFDRNNRLHATWVYREVAETWASNHDLHYAYSDDGGATWFNN
                     SGEKIADMSADDPIELNDAGIVVREIPVFSWLMNAGCMALDSKGRPHVMTFKLPEPRE
                     PNQLKHDPPDVIRHDLHFVHYWRNEDGTWHGGEPIRPINGDIPGESVARGDIVFGPDD
                     TLFFYWVDRNEGRFRYLVASAADQWQAWQVDWLLDKGFSGGDASKHDRRRWQNEGVLS
                     FTARFGDQGFGIVDLKMSQGAAP"
     gene            complement(1444..3900)
                     /locus_tag="PKI05_00990"
     CDS             complement(1444..3900)
                     /locus_tag="PKI05_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50798.1"
                     /translation="MQQLRMFVLGMFLATASAGSLAQEAVNVPATEEQRQYVCISGIY
                     PHLAVFNQPEDPSDRPQHGECGIGAIAVWADRLWYLTYPQHKTMGSNDKLYELDADMN
                     VVIRPESVGGTHAARMIHRESEQLILGPYFIDKQRNVRAANLKELRGRMTAIMRHLDD
                     PANKVYFFDMEGAIYEVDVHTLAVNRLFVKPVPGWHGKGGYTAQGRVVIANNGEVGPA
                     DGYRHVLVGGPAQGEEAGVLAEWDGKTWRIIERKQFCDVTGPGGIEGSPSDDAPLWAI
                     GWDKRSVILKLLDHGQWFTFRLPKGSHTFDPRHGWFTEWPRIREIASGRLMLCMHGQM
                     FDFPRGFSAQACGGIRPVCTHLRYIPDFCHWNGRVVLGADDASMMHNPMCGQGQSNLW
                     FGTPEDLRRFGPTMGWGGVWMDDPVTADMPSDPFLIAGYKQRVVHIAQRGDRAVTFYF
                     EVDRAGNGQWEKLFDVEVQPGTYRWSVIPEDVAAEWVRVRASADCRATVYFHCWSPRP
                     AKADETTLFAGLADVSSSVEHYGGLIRPAAHNRNLQWLVWKGNAKGEIQRLGYHEVDI
                     TREGRPILVNPKEDRTEEVEKIASLKADFEVDTASVIVRDTSGQRWRLPRGPAAFDQW
                     VGQMRAVREVASERFLANIHGTFYEIPRDAQNQPDWSKMKPVCSHSKRIIDFCSWRGL
                     LVLAGVADNAAEDGQVFGNPGGPRLWFGCLDDLWKLGPPRGKGGPWNNTAVRPGEPSD
                     PYLMTGYARKTLVLSHDADEPVRFTVEIDFDHTGFWRYAEFEVLPGKPFRHDFGDFHA
                     HWIRFRCDKPCRATTWLEYE"
     gene            complement(3973..5556)
                     /locus_tag="PKI05_00995"
     CDS             complement(3973..5556)
                     /locus_tag="PKI05_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007332548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA50799.1"
                     /translation="MHFSIKRGFGRRVGTIGAILLGLLLFECCGLSCLAAERRPNFLF
                     IIVDDQSPNDLKIYNPRSILNTPNIERLAAEGVVFDGAYIMGSWVGAVCLPSRTMIMM
                     GRTVWHIPAPGKNPANPHTNNPKLVPPDLPDHTLPAVFNRAGYATMRTCKIGNSYEAA
                     NKLFTVRRDATKRGPTDETGSAWHAEQVLEYLAERESRGDKAPFLIYFGFSHPHDPRD
                     GKPELLARYGAVNHTDRNTLPPAHPNQPLLPVNYLPAHPFDTTHMDVRDEVAVSGVWD
                     RRDERIIRNELGREFACAENIDIQIGRVLRRLEEMGELENTYIFYTADNGMALGSHGL
                     QGKQNLYQHTWRVPLIVKGPGIPGGRRVEGNVYLLDVLATLCDLAGIEPPATYEGISF
                     RPVLEGKTDKIREVLYGVYCGGAKPGIRCVKKGDWKLIKYDAPELGVHVTQLFNLAEN
                     PHEFLIEHHNPNVTALTGVVPEPHQVSLADDPRYAAKRAEMEALLLAEMRRLDDPYRL
                     WDQPDDGLRMPQRQSLSKE"
     gene            complement(5749..6612)
                     /gene="ilvE"
                     /locus_tag="PKI05_01000"
     CDS             complement(5749..6612)
                     /gene="ilvE"
                     /locus_tag="PKI05_01000"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HOA50800.1"
                     /translation="MSLKVYINGKYYDKEEAKISVYDHGLLYGDGVFEGIRSYGGKVF
                     RLREHLDRLWDSAKAIWLTIPMTKEEMAAAIEETLRINGIEDGYIRVVVTRGVGTLGL
                     DPNRCHQPQVIIITDHISLYPKEYYENGLEIITVSTIRNHPAAVSPRIKSLNYLNNIL
                     AKIEGLQAGCIEALMLNHKGEVAECTGDNIFIVRRGELLTPPREAGILEGITRAAVIE
                     LAQQANIRVLEVPLTKHDIYIADECFLTGTAAEIVPVVKVDSRPIGDGKPGPITRDLM
                     ERFHRLTRNHC"
     gene            complement(7161..7592)
                     /locus_tag="PKI05_01005"
     CDS             complement(7161..7592)
                     /locus_tag="PKI05_01005"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOA50801.1"
                     /translation="MTIGQETLVLLDTSVVLHLARNDTTGRAIESRFRLTSRRDRPLI
                     STVTEGELLALAYSRQWSPKKLEALRKMLENFVRVDAGLAPVVEWYAKLDAEARRRGA
                     PRGQNDLWIAATAKATGAVLLTCDKDFDWLDPDYLKVFRMP"
     gene            complement(7589..>8035)
                     /locus_tag="PKI05_01010"
     CDS             complement(7589..>8035)
                     /locus_tag="PKI05_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50802.1"
                     /translation="RSVLEIAERFCAATPPPERVRLAGTLDMVRASTRTFGIRLEQGE
                     ELRGVLLEEDIHKIAPLLGKQVLVLGRVQYKPSGRALRIDAEEVKPAHGDVSLWSRIP
                     PARGQPINASTLRRPQGPRSGLAAVIGRWPGDESDHEIEAWLEEIS"
BASE COUNT         1724 a         2354 c         2210 g         1747 t
ORIGIN      
        1 ccgcatccga ttttttcttc tgtttacacc tttgatcatc gggggctgct ggtggggagt
       61 aaacatgcag gatgccacgg ctgccgagtc ggcatgggtt cttcgtgctg cggttcctct
      121 tgccgagaag acatggcgct ggaattgggc gagctgggac caggagaaga ttgtcacgtt
      181 cggagagtac caatatacgg tgttttggga tgcggacggc gttttcgtcc tcgccagacg
      241 ccacctcgca gacaataccg tacaacttgt ccgaatgcca gggtggaggc tctcggtaga
      301 cgatgcccac cgcaatacct gcctgggcat ttccgaaaag gacggtcgtt tgcacttttc
      361 ctgggaccat cacaataatc ccctcaatta cgggcgaacc cgcgcgggct ttctcacgca
      421 gccgccggcg cagatctcag ccggcgactt tgaaccacgc cagcccccgg ccgaaggcga
      481 ctttcccatg tctcgtgtga cctatccccg cttcatcaac gataacaagg gaggccttta
      541 cctgttttat cgcgaaggcg gttccggcaa tggggataat tacttgtggc gatacgacgc
      601 cgaaacggga cgctggacaa tggtgggatg tgtctttagc aaacggggca cctacgcacc
      661 ctggaacaac agcacctccc gcaatgccta ttttcacgat cttctcttcg accgcaacaa
      721 tcgacttcat gccacttggg tatatcggga agttgccgaa acctgggcca gcaatcacga
      781 cctgcactac gcgtacagtg atgatggtgg ggccacatgg ttcaacaatt caggcgagaa
      841 aatcgccgat atgtccgccg atgatcccat cgaattgaat gacgcgggca tcgtcgttcg
      901 agaaattccc gtgttttcgt ggcttatgaa tgccggttgt atggcgctgg attccaaagg
      961 gcggcctcat gtcatgacat tcaagcttcc cgagccgcgg gagccaaacc agctcaagca
     1021 cgatcctccg gatgtcatcc ggcacgacct gcactttgtt cattattggc gcaatgagga
     1081 cggaacatgg cacggaggag aaccgatcag accgattaac ggagacatcc ccggcgaaag
     1141 cgtggcccgc ggcgacatcg tgtttggccc ggacgataca ctttttttct attgggtcga
     1201 tcgaaacgag gggcgatttc gctacctggt ggcttccgcc gccgatcagt ggcaagcgtg
     1261 gcaagttgat tggcttctcg acaaaggctt cagcggtggt gatgcttcca aacatgaccg
     1321 ccgccgttgg caaaacgaag gtgtgctttc ttttaccgca cgattcggcg accaaggttt
     1381 tggcatcgtg gacctgaaga tgtcgcaggg agccgccccc tgagcggatc acctcatagc
     1441 ggcttactcg tactccagcc aagtcgttgc gcgacacggc ttgtcgcacc gaaaacgaat
     1501 ccaatgggcg tggaagtcgc cgaaatcgtg acggaacggc ttgccgggga gaacctcaaa
     1561 ctctgcgtac cgccaaaagc ctgtatggtc gaaatcgatc tccaccgtga aacggaccgg
     1621 ttcatcggcg tcgtgcgaca acacaagtgt cttgcgggca taaccggtca tgaggtaagg
     1681 atcggatggt tcgccgggac gcacggccgt gttattccac gggccgcctt ttcctctcgg
     1741 tggaccaagt ttccacagat cgtccaaaca cccaaaccat aagcgggggc cgcccggatt
     1801 tccaaacact tgcccatctt cagcggcgtt gtccgcaaca cccgccagga cgagaagtcc
     1861 gcgccatgag cagaagtcga tgatccgctt ggaatgacta cagacaggtt tcattttgga
     1921 ccaatccggc tggttctgag catctcgagg aatctcgtaa aacgtcccgt ggatgttggc
     1981 caggaatcgt tcggaggcca cctcgcggac tgcccgcatt tgtcccaccc attgatcgaa
     2041 agcggccggt ccgcggggga gccgccaccg ctgtccagag gtgtcgcgaa caatgacgga
     2101 ggccgtatcg acctcgaagt cggccttcag cgaagcaatc ttttccactt cctccgtgcg
     2161 gtcctccttg gggttgacaa ggatgggtcg gccttcgcgg gtgatgtcca cctcgtgata
     2221 gcccaggcgc tggatctcgc ctttggcatt tcccttccag acgagccact gaaggttccg
     2281 attgtgagcg gccggccgga tcaagccccc gtagtgttcc accgatgacg aaacatcggc
     2341 caatccggca aacagcgtag tttcgtcggc cttggcgggc cggggggacc aacaatggaa
     2401 atacacagtg gcccgacaat cggcggaggc ccgtacccgc acccattccg ccgccacatc
     2461 ctccgggatt acgctccagc gatacgtccc cggctgtact tcgacgtcaa agagtttttc
     2521 ccactggcca ttgcctgcgc ggtccacctc gaaatagaaa gtgacggctc gatctccccg
     2581 ctgtgcgata tgtaccaccc gttgcttata accggcgatg aggaagggat cggacggcat
     2641 atcggcggtg acgggatcat ccatccagac acctccccaa cccatcgttg ggccaaaccg
     2701 gcgtaaatcc tcgggcgttc caaaccacag attggattgt ccctggccac acatcggatt
     2761 gtgcatcatc gaagcgtcgt cggctccgag gacgacgcgg ccgttccagt ggcagaaatc
     2821 ggggatgtat cgcaggtgcg tacagacggg acgaatgccg ccacacgcct gggcggaaaa
     2881 ccctcgggga aaatcaaaca tctgcccgtg catacaaagc atcaatcggc ccgatgcgat
     2941 ttcgcgaatc ctcggccatt ccgtaaacca tccatgccga ggatcgaacg tatggctacc
     3001 cttgggcagg cggaaagtaa accactgccc gtgatccaat agcttgagaa taaccgatcg
     3061 cttgtcccaa ccgatagccc aaagcggggc atcgtcgctg gggcttcctt cgataccacc
     3121 gggtcccgtc acatcgcaaa actgtttacg ctcgatgatc cgccatgtct tgccgtccca
     3181 ttcggcgagg acacccgctt cttcgccttg ggcagggcca cccacgagga cgtgacgata
     3241 accatcagcg ggcccaacct ctccgttgtt ggcgatgacc acacgccctt gggccgtata
     3301 gccgcctttg ccgtgccacc ctggaacggg cttcacgaaa agacgattca ccgccagcgt
     3361 gtgaacatca acctcataaa tggcaccttc catgtcaaag aaatagacct tgttagcggg
     3421 atcatccagg tggcgcatga tcgccgtcat acgcccacgg agttccttga ggtttgccgc
     3481 ccggacattc cgttgcttgt cgataaaata tggtcctagg atcagttgct ccgattcgcg
     3541 gtggatcatc cgggccgcat gggtgccgcc gacgctttcc ggtcggatga ccacattcat
     3601 gtcggcatcc aactcgtata acttgtcgtt actgcccatc gtcttgtgtt ggggataggt
     3661 gagataccac agacggtcgg cccaaacagc gatggctccg atgccacatt caccgtgctg
     3721 cggccggtcg gaggggtctt ctggctgatt gaacacggcc aaatgcggat aaatgccact
     3781 aatgcacaca tactgccgct gttcctcagt tgccgggaca ttgaccgcct cttgtgcgag
     3841 cgaccccgcc gaggcagtag ccaggaacat gcccaacacg aacattctca gttgttgcat
     3901 agatgggtcc tccaatgctt gtgattatcg tgcgattgtg aaatcatgtg ggtcgtggca
     3961 gcctcgtgcg catcactcct tcgacagcga ctggcgttgg ggcatccgta agccatcgtc
     4021 gggctggtcc cacaaccggt acggatcatc aagacgccgc atttcggcca gaaggagggc
     4081 ttccatctct gcgcgcttgg ccgcatagcg gggatcatcg gccagactca cctgatgggg
     4141 ctccgggaca acgccggtta aggccgtgac gttcggattg tgatgttcaa tcaaaaactc
     4201 gtgaggattc tcggccaaat tgaaaagttg cgtgacgtgg actcccaatt cgggggcgtc
     4261 atatttgatg agtttccaat cccctttttt cacgcagcga atgcccggct ttgccccgcc
     4321 gcaatatacg ccataaagca cttcgcggat cttgtccgtc ttgccttcta gaacgggtcg
     4381 aaaactgatt ccctcatagg tcgccggggg ttcgatgccg gccaaatcgc acagcgttgc
     4441 taagacgtcg aggagataaa cgtttccctc cacgcgacgg ccacccggaa taccgggacc
     4501 tttgacgatg agaggcacac gccaggtatg ctgataaagg ttttgctttc cctgcaagcc
     4561 atgagaccca agggccatcc cattatctgc cgtataaaag atataggtgt tctctagctc
     4621 ccccatttct tccagacgtc gcagcacacg gccaatctgg atgtcgatat tctcggcgca
     4681 agcaaattct cgccccaatt cgttacgaat gattctctcg tcgcggcgat cccaaacacc
     4741 actgacggca acctcgtccc gaacatccat gtgtgtggtg tcaaacgggt gggccggcag
     4801 gtaattaacc ggcaataggg gctgattggg atgggcgggc ggtaatgtgt tgcgatcagt
     4861 atggttaacg gcgccgtagc gggcaaggag ttccggcttt ccatcgcgtg ggtcatgcgg
     4921 atgcgagaat ccaaaataga tgagaaacgg ggccttatca ccacgagatt cccgttcggc
     4981 cagatattcg aggacctgct cggcgtgcca ggcactgccc gtttcgtcgg tgggaccccg
     5041 ctttgttgcg tcccgcctta cggtaaacag tttattcgct gcctcgtagc tgttgccgat
     5101 cttacaggtc cgcatcgtag cgtagcccgc tcggttgaac acggccggca aggtgtggtc
     5161 cggaaggtct ggcgggacga gcttagggtt atttgtatga ggattggcgg gattcttccc
     5221 cggcgccgga atatgccaca ctgtccggcc catcatgatc atcgttcgcg atggcaaaca
     5281 aacggctccg acccacgagc ccataatgta ggcgccgtcg aagaccaccc cttccgcggc
     5341 cagtcgttca atgttcggcg tgttgaggat cgatcggggg ttatagattt tcaggtcatt
     5401 gggcgattgg tcatcgacaa tgatgaataa aaaattggga cggcgttcgg ctgcgaggca
     5461 tgatagtccg caacattcaa ataggagaag cccgagcagg attgcaccga ttgtcccaac
     5521 gcgcctgcca aaaccgcgct tgatgctgaa atgcatggaa aaccacctcg ccggacaaca
     5581 cgtgactgcc aaaacaccca aaccatcgcc cgatccggct gataatcttt cagccagctg
     5641 cccagtcata acagcggtta tgccagtggc aaaacgtttt cgacaaagtc attgctaaat
     5701 ggaacggttg caaacccgtt tgccccgtcg tgccccgctg tcagggaatt aacaatgatt
     5761 cctcgtcagt cggtggaatc gttccatcaa gtcacgagtg atcggaccgg gctttccatc
     5821 gccaatgggc cgactatcga ccttgaccac gggcacgatt tccgccgctg ttcctgtcag
     5881 gaaacattca tcggcaatgt agatatcgtg ttttgtcagg gggacttcaa ggacacggat
     5941 attggcttgt tgggctagct caatgacggc tgcccgtgtg atcccctcta gaattcccgc
     6001 ctcgcgcggc ggtgtcagca attcaccacg ccggacgatg aagatattat cgcccgtgca
     6061 ctcggccacc tcacccttgt ggttgagcat caaggcctcg atgcagcccg cctgaagccc
     6121 ttcaatcttg gccaagatgt tattgaggta attgagggat ttgatccgtg ggctgacggc
     6181 ggccgggtgg ttgcggatcg tgctaaccgt gataatctcc aagccgttct cgtagtattc
     6241 cttgggatac aaagagatgt gatccgtgat gatgattacc tgcggctgat ggcagcgatt
     6301 aggatcgagg cccagggtcc caacgccgcg ggtcaccacc acgcgaatgt agccgtcctc
     6361 gattccgttg atacgaaggg tctcttcgat ggccgctgcc atctcctctt ttgtcatggg
     6421 aatcgttaac caaatggcct tggcggagtc ccacaagcga tcgaggtgct cgcgaagccg
     6481 aaagaccttt cccccgtagc tgcggattcc ttcaaagacg ccatccccgt aaagtaggcc
     6541 gtgatcgtaa acgctgatct tggcctcctc tttgtcgtaa tactttccgt tgatgtaaac
     6601 cttcagcgac atggcgtgat cttagacctc tagttttgcc aagaaattaa gccgttggaa
     6661 cgttatgcag acaaactatt agcgtaccat gccagagatc gcttggcgag acgccggggg
     6721 cacttttagc ggtaagatgt ccgcatgaac gtagcactac ggtggagttg aaataaagcg
     6781 atccgccgtg ccttgcacta cggagctatt cagaacttac cgcataacct cccctctccc
     6841 gttcggcgaa gaggggccgg gggtgagggt ggctggaatc gctagcgctt tctcgatcga
     6901 ccccgacgat cgcttgccct caccccaacc ctctcccaga aggagaggga actttgcgat
     6961 agtctctagg cggatttcgg cattgccaga cagttcggcc tatcaaacat tgcagtttgc
     7021 acctccatat tgcgaggcgt gaaaagggag cgcgactttg ctgtacaagc taactctttt
     7081 gccgccggac gctttgcaac acccctcaag cagcaccttg gaaggggcct ccggccggcg
     7141 aattgcaaga gttcagctat tcagggcatc ctgaagacct ttaagtagtc tggatcaagc
     7201 cagtcaaaat ccttatcgca tgtcaaaagc acggctccag tcgccttggc tgtggcagca
     7261 atccaaaggt cattttgtcc ccgcggggct cctctgcgtc ttgcttctgc gtcaagcttt
     7321 gcgtaccact caaccaccgg tgccagcccc gcatccacac gaacgaaatt ttcgagcatt
     7381 ttgcgcagcg cttcaagctt cttcgggctc cactgcctgg agtaagccaa agccagcaac
     7441 tcgccttctg taacggtaga gatgagcggc cggtctcgcc gcgatgtaag tctgaaacga
     7501 gactcgatgg cccggcccgt cgtatcgttt cgggcaaggt ggagcacgac agaggtgtcc
     7561 aggaggacaa gcgtttcctg cccgatggtc acgagatctc ctccaaccaa gcctcgatct
     7621 cgtgatcgct ttcatctccc ggccagcgcc cgatcacggc cgccaaacca gacctcgggc
     7681 cttgggggcg gcggagcgtg ctagcgttaa tcggctgacc tctcgccggt ggaatccgag
     7741 accagaggga aacgtccccg tgggctggct tcacctcctc tgcatcgatc cgcaatgccc
     7801 gccctgacgg tttatattgt acccgcccga gcacaagaac ctgtttgccc aataatgggg
     7861 ctattttatg aatatcttct tcgagaagca cgccgcgcaa ctcctcacct tgttccaacc
     7921 gaataccaaa ggtgcgcgtg ctagcgcgga ccatatcgag cgtccctgcc aggcgaaccc
     7981 gttcgggcgg cggcgtggcc gcgcaaaatc tctccgcgat ctccaacacg ctcct
//
LOCUS       DAOPTR010000026         8000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_7508_length_8000_cov_3.680680, whole genome shotgun sequence.
ACCESSION   DAOPTR010000026 DAOPTR010000000
VERSION     DAOPTR010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 8000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8000
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7508_length_8000_cov_3.680680"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="PKI05_01015"
     CDS             complement(<1..804)
                     /locus_tag="PKI05_01015"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOA50803.1"
                     /translation="MLTRREMLGVTTGTLTGLGLFSGVARAGSNSLEPVREEGIAWYD
                     VTQWGVEGRGWNDTARFFDRLPARAEKVVRPAVWNLSRHSAGMCARFVTDATLIRVRY
                     SLLSANLAMPHMPATGVSGLDLYAQDDRGVWRWVAVAQPTKQEMDLTLVSGVRPGKRP
                     FMLYLPLYNGVESLQIGVPENADFQPVPPRTDGLIVYYGTSIAHGACASRPGMAFPAI
                     LGRRVNRPVINLGFSGNGRMDLELVPFLGELDAAVFCIDCLPNMQADEIK"
     gene            complement(975..2096)
                     /locus_tag="PKI05_01020"
     CDS             complement(975..2096)
                     /locus_tag="PKI05_01020"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOA50804.1"
                     /translation="MSATRLILVGGFLGAGKTTLLTEAATRLIRRGKRVGLLANDQAP
                     DLVDTEVFKTSGAGVEEVAGGCFCCRFPDMITAMERLLQHSRSDVILAEPVGSCTDLS
                     ATVLQPLKDRYRDRFEVAPFSVLIDTTQVCALARLPATTVPAAPPRFPDKVLYIYEKQ
                     LEEADAIVLNKVDLLSPEEVSGLKQALATRFPEVPVFTMSALTGDGVDAWLDFVTQSQ
                     AAGRRIAQVDYDTYAAGEAALGWMNASARLRASTPVDWRTFAADTLDAIRTRLRNASG
                     EIAHLKIFLTTPGAYVAGNITSNEGPVSVRGEIDAARSTVSLLINARVHAHPDILRAI
                     VEEGLRATAGDRVEVTITNIRSFFPGRPEPTYRYRNTVQ"
     gene            complement(2397..2816)
                     /locus_tag="PKI05_01025"
     CDS             complement(2397..2816)
                     /locus_tag="PKI05_01025"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOA50805.1"
                     /translation="MLDLSSCPCAGVTLDRLIQPAILTVLAEGPRHGYAVAEEINRRM
                     AFGKETPDISGIYRFLKLMEERGLVVSSWDVSHGGPARNCYEITPEGLRCLAQWIKTL
                     ERYRDGISKLLRAARSASKKAESRMKKIRLKQGHRIT"
     gene            3015..5918
                     /locus_tag="PKI05_01030"
     CDS             3015..5918
                     /locus_tag="PKI05_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50806.1"
                     /translation="MGFCPVSRGFHLGGQRTRLECVTMIVWVVAVVALNACGANEAGE
                     TGPLTRRVPLEANMLKGETEFVGNVAALVDEQSLVGDPPTGKPVTGWDFDYRYTQTLP
                     HCVLIDLAVERPIARVWFFDTFNVGKLRLEVGEPGKWQEVATVTTERFQSWVPISVDR
                     TCRYLRLSRLDAAANIAEIAIDEYTPAGLAELEARREAARRREAALRAAAEEARHRPR
                     IDLGPPFGELPLVDEINCGSDSQGHAFSENPVGVSEVRSILGRPCRVLRPLPDRAAYV
                     AYRIGQFKLLRPGGTYVLVIDYPEDAPRHWVIINGGCETRIGFHTGATLPDALHAKYV
                     DSLPESVRLPLSGEYRQWKMVFRLHDRFPQLGTPRGEATRPLLPEDGFPVIIAQFSSP
                     NIPLSQGAAVSRIRLFEVPDETRLECPIRFPPEPLPRRHIFWREEMADGVIASRNEEK
                     RGVRNPLDWYRFKLSTMKFLGIPTYCKDLLEFGACQGWDPTPYGGNRWVHFNDFHKGL
                     WEQIVEMMGQAGKEILPYYEYAGSRGDQGLGYQRRAKPLTRDDAFTHISWVESANADI
                     TDPETWGDFRKMLDLTVARLAHKARFVGVWLRPRGQLPIGFGDATRKRFAEETGLNHT
                     PSREELQSDTILCKQYYDWWFEKRREFLIHVRDYIREHGAQPDAVVLYTSLLAESGPS
                     FPTWDKILVTDDPATWQTLLQTPLYGQGKKWRVLSLSEVVERGLYLEALLSPPLNWGG
                     WEWHHADPAPDPERYASTKGVLMTHAFNRFYTVLSPQTFDRFRGPSGLAIVRHYPLNE
                     DMMLDEADKPILDYFTADIEHAGPYCMAEEALAVAHGDPWYIGYLTGNSLARGFPETV
                     RAFNRAFLSLPALPSEVVSGLTSDKDIVVRRIATEGYGTYFALVNTASCDKPRVALRL
                     PEGTYEDTVSGKILRADDVATHDFVPFELWTIRLLPRDSNQSPAVAPSTDTTP"
     gene            6275..7660
                     /locus_tag="PKI05_01035"
     CDS             6275..7660
                     /locus_tag="PKI05_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOA50807.1"
                     /translation="MKWQLWQSALRRFIVTGVVGLTVLCGLWYFGTGLQAEEALPAPA
                     APVQPPAKDVEIKPLEGTPQELLKFLEDLNAQQGPADPQAMMEFQRKRAKVFIEVADK
                     ILLQDKLPAETLDGAFQFKFRGLLVLLQMGDTQARELLPKLAEQLMAKAEEILKGTPT
                     EDAAQRAVQWAMMAPRLGGPDFLPRVIALPKRLEELGFRQLATQLRGGLLMLRLRMGQ
                     AEDRAELAKDVTAFLEDAWTRFSQAEKITHEDVAMVVPVLQYLEFARLDVNLSDYYGR
                     FGGLFAKNADEQIAELGQQLQGISRRLGLVGKKMELAGKTVDGKDFDWSQYRGKIVLV
                     DFFATWCGPCRAEMPNVKENYERFHSKGFDVVAISIDENRADLERYLQENKLPWVVIH
                     NQDPKAQGPANPATYYGITAVPTVMLVDKDGTVLTFDARGPQLGKKLEELLGPPEPPA
                     KDEKQGTTEKQ"
     gene            complement(7814..>8000)
                     /locus_tag="PKI05_01040"
     CDS             complement(7814..>8000)
                     /locus_tag="PKI05_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50808.1"
                     /translation="ADWGPSDPATLHDCIFEFRRRGHSLQEALEVFYNNPVRFLSQNP
                     KFDLPLVKLETVGPNAG"
BASE COUNT         1747 a         2192 c         2300 g         1761 t
ORIGIN      
        1 tttgatttcg tcggcctgca tattgggtag gcagtcaatg cagaacactg ccgcgtcaag
       61 ttcgcccaga aatggaacga gttctaggtc catacggcca ttgccagaaa agcccagatt
      121 gatcacggga cgattgactc gccttcccaa aatggcggga aatgccatgc ccggcctact
      181 ggcacatgcg ccatgagcaa tggaggtccc gtaatagacg atcaagccat cagtccgcgg
      241 cggtactggc tgaaaatcgg cgttttccgg gacaccaatc tgcaaggact caacgccatt
      301 gtagaggggc aaatacaaca tgaatggacg ttttccagga cgtacgccgc tgaccaatgt
      361 caggtccatc tcctgttttg tgggctgcgc cacggcaacc caacgccaca caccgcgatc
      421 atcctgagca tacagatcga ggccactcac cccggttgcc ggcatgtgcg gcatggccag
      481 gttagcagaa agtaacgagt aacgcacgcg gatgagtgta gcatccgtca cgaatcgggc
      541 acacatcccc gccgagtgac ggctaagatt ccaaaccgca ggccggacga ccttctccgc
      601 ccgggcgggg aggcggtcaa aaaagcgagc cgtatcgttc cagccacgac cttccacacc
      661 ccattgggtc acatcgtacc acgcaatgcc ttcttcccgc accggctcga gtgagttcga
      721 accagcccgc gccactcctg aaaacaaacc caacccggtc agcgttcccg tggttacacc
      781 tagcatttcg cggcgtgtca acatggcgga ttccttagca aaagacttgt ggggccatca
      841 attccgcctt tatttcgatc aaccgagcca acggctgaaa ataccaggga actcctggcc
      901 aggctcacga cacttcaggc tgtcttcgat tgactggggt gacgttttgc ccaagcagca
      961 taaaaaagat gccgctattg caccgtgtta cggtaacgat acgtgggctc cggacggcct
     1021 gggaaaaaac ttcggatgtt ggtgatcgtc acctcgacgc gatccccggc ggtcgcccgc
     1081 aggccttctt cgacaatggc ccgcaagatg tcgggatgcg cgtggacccg ggcattgata
     1141 aggagggaaa cggtactccg cgcggcgtcg atctcccccc ggacggatac gggcccctca
     1201 ttacttgtga tattgcccgc cacataagca cccggggtgg tgaggaaaat cttaaggtgg
     1261 gcaatctcgc cggaggcgtt gcgaagtcgc gtgcggatgg cgtcgagtgt gtccgcggcg
     1321 aacgttcgcc aatcgacagg cgtggaagca cgaagtctcg ccgaggcatt catccatccg
     1381 agggctgctt cccccgccgc ataggtgtcg taatcaactt gggcaatcct tcggcctgcg
     1441 gcctgacttt gggtgacgaa atcgagccaa gcgtccacac cgtcgccggt gagcgccgac
     1501 attgtgaaca ccgggacctc tgggaagcgg gtggccaggg cctgcttcag cccagaaacc
     1561 tcctccggcg agaggaggtc aaccttgttt agcacaatcg catcggcctc ctcgagttgc
     1621 ttctcgtaaa tgtacagaac cttgtccggg aatcgtggcg gggcggcagg cacggtggtt
     1681 gccggtagcc gtgccagcgc acaaacttgc gtggtgtcga tgagcacgga aaatggcgca
     1741 acctcaaacc gatcgcgata gcggtcctta aggggctgga gaacagtggc cgataggtca
     1801 gtgcagctac cgaccggctc ggcgaggatg acatcgctgc gcgagtgttg gagtaatcgc
     1861 tccatggcgg tgatcatgtc gggaaatcgg cagcaaaaac agccgccagc tacctcttcc
     1921 acacctgcac cgctcgtctt gaaaacttcg gtgtcaacga gatcgggcgc ctgatcattg
     1981 gccaggagcc cgacacgctt gcctcgacgg atcaagcgtg tggccgcctc tgtcaaaagg
     2041 gtcgttttcc ccgcccccag aaaaccacca actaaaatca atcgtgttgc cgacataaga
     2101 tcactctcca acgggacgag gacctcacca tcaaatacaa gccaaattct gggtcaataa
     2161 taagagccta tcccataatc tcccctctcc cgttcggcgg gagagggggc gggggtgagg
     2221 gtggctcgaa tcgctgccgg ttttctcgat cgactccggc gatcgcttgc cctcacccca
     2281 accctttccc agagggagag ggggactttt tgggatagac tctaaatgcc gttccacttg
     2341 gcgatctgtt tttcaggaag gctcaacctt acggtgatca cggtagcttc cctcggttag
     2401 gttatccgat ggccttgttt taacctaatt tttttcatcc tgctttccgc tttctttgag
     2461 gcactgcgtg ctgcccgcaa gagcttgctg atcccatccc gataacgttc cagcgttttg
     2521 atccattgcg cgaggcagcg tagcccctca ggcgtgattt cgtagcaatt cctcgccggc
     2581 ccaccatggg acacatccca ggaagaaacg accagccccc tttcctccat gagtttgagg
     2641 aagcggtaaa tgcccgaaat gtcgggagtc tctttaccaa aagccatgcg gcggttgatt
     2701 tcttccgcca cggcgtatcc atgtcgaggt ccctcggcga gcacagtcag gatggccggc
     2761 tgaataagcc gatctaaagt gactcccgca caaggacaac tgctaagatc caacatagtc
     2821 cttcctcata tattggagtt attcgaataa tcgcgttatt tagcgtagtt acccaaaaag
     2881 cacgtgtcaa gcgactgtgt cggcctgcat aaggagcctt gttggctaga ttgtccaaag
     2941 cttctttggt aactagactt tcaaggaatg tcttgcaatc cctcgttgtt gtcttcatca
     3001 gggagttcac atcaatggga ttttgtccgg tatctcgtgg cttccatttg ggtggccagc
     3061 gaacacgctt ggagtgtgtg acgatgatag tgtgggtagt cgcggtggtg gcgctcaatg
     3121 catgcggcgc aaatgaagct ggagaaaccg gcccgttgac gcgtcgcgtg ccactggaag
     3181 cgaatatgtt aaagggcgaa acggaattcg taggcaacgt ggcagccttg gtggatgaac
     3241 aatcgctggt gggcgatccc ccaacgggga aaccggtgac ggggtgggac ttcgactacc
     3301 gatacacgca aacgttgccc cactgtgtgc tgatcgattt ggcagtcgaa cgaccaattg
     3361 ccagagtgtg gttttttgac accttcaatg ttgggaaatt gcggctggag gttggcgaac
     3421 cgggaaagtg gcaagaagtc gccacggtga ccaccgagcg ttttcagagc tgggtgccca
     3481 tttccgttga ccggacctgc cgttatttga ggttatcgcg gttggatgct gcggcgaata
     3541 ttgccgagat tgccatcgac gagtacacac ctgcgggctt ggccgaactc gaggcacgtc
     3601 gggaagccgc ccgccgacga gaggcagctc tccgtgccgc cgcggaggag gcccgccaca
     3661 ggccccgcat cgatcttgga ccaccctttg gagagttacc cctggtggat gaaatcaact
     3721 gtggaagtga ctctcagggg cacgcctttt ctgaaaatcc cgtgggtgtg tcggaagtcc
     3781 ggtcgattct tggacggcct tgtcgcgttc tacgtccctt gcccgaccga gccgcgtatg
     3841 tggcctatcg gattggtcag tttaagctct tgcggcctgg tgggacctac gtgttggtca
     3901 ttgactatcc ggaggacgct ccgcgacatt gggtcatcat caatggaggt tgcgaaacgc
     3961 ggattggttt ccacacggga gctacgcttc ccgatgcgtt acatgcgaag tatgtggact
     4021 cactaccgga gtcggttcgc cttccccttt ccggagaata ccggcagtgg aagatggtct
     4081 tccgactcca cgatcgcttt ccccaattgg gcacccctcg cggggaagcg accaggcccc
     4141 tcctccccga agatggcttc ccggtcatca ttgcccaatt ttcctcgccg aatattcctc
     4201 tgtcgcaagg ggcagcggtg agccgcatcc ggctgttcga ggttcccgat gagacacgtc
     4261 tggaatgtcc catccgtttt ccaccggagc cgttacctcg gcgacacatt ttttggcgag
     4321 aagagatggc cgacggtgtg atcgcttctc gtaatgaaga gaaacgtgga gtgcgcaacc
     4381 cactcgattg gtaccgcttt aagctctcca cgatgaagtt cctgggtatt ccgacttact
     4441 gcaaggattt gttggaattc ggcgcatgcc aaggatggga tcccacaccc tacgggggta
     4501 atcgctgggt gcatttcaac gactttcata agggtctttg ggagcagatt gttgagatga
     4561 tggggcaagc cggaaaggag attctgccct actatgaata cgcaggcagc cggggagatc
     4621 aggggctcgg atatcaacgc cgagcgaaac cgttgacgcg ggatgacgct tttacccaca
     4681 tttcctgggt cgaaagcgcg aatgccgata ttacggatcc agaaacgtgg ggagattttc
     4741 gaaagatgct ggacctgacg gtagctcggc ttgcccacaa ggcacgcttt gtcggcgttt
     4801 ggttgcgacc tcgtggccaa ctacccattg gtttcggcga cgcgacacga aagcgctttg
     4861 ccgaagaaac cgggctaaat cacacgcctt cgcgtgaaga actgcaaagt gatacgatac
     4921 tttgcaagca atactacgat tggtggtttg aaaaacggcg tgagttcttg attcacgtgc
     4981 gggattacat ccgcgaacat ggcgcccaac cagatgccgt tgtgctctac acctcgttgc
     5041 ttgcggaatc tggcccgtcc tttccgacct gggacaagat tctcgtaact gatgacccgg
     5101 ccacctggca aacgctgttg caaacgccgc tatacggtca aggtaaaaaa tggcgcgttc
     5161 tttcactctc ggaagtggtc gagagaggcc tgtacttgga ggcccttctc tcgccccctt
     5221 tgaattgggg aggatgggaa tggcatcatg cggaccctgc tcccgatccc gagcgttatg
     5281 cctcaacaaa aggcgtcctg atgacacacg ccttcaatcg tttttatacc gtgctctcgc
     5341 cgcaaacgtt tgaccgcttt cgcggtccca gtggattggc cattgtccgt cactaccccc
     5401 tcaacgagga tatgatgctt gacgaggccg acaaaccgat cctggattat ttcacggccg
     5461 acatcgagca tgcggggcct tactgtatgg ccgaagaggc cttggctgtg gcccatggcg
     5521 acccgtggta tatcggctac ttgacgggaa attcgcttgc ccgcggtttt cccgagaccg
     5581 tccgcgcgtt caatcgggcg ttcttgtcgt taccggcgtt acccagcgag gtcgtctcgg
     5641 gtctgacctc cgataaggac attgtggttc gccgtattgc gaccgagggc tacgggacat
     5701 attttgcctt ggtaaacaca gcaagttgcg acaagccccg cgtggccctt cggctgccag
     5761 aaggcactta cgaggatacc gtttcgggca aaatccttcg tgctgacgac gtggccacgc
     5821 acgatttcgt accctttgag ctttggacga tccgacttct tccgagggac tcgaaccaat
     5881 cgcccgcggt ggctccctca acggacacga ctccctgagt gtggcatgca tgtcatgggc
     5941 gatggataga gaagacagtg tttatacctt cctcgggcat catagcgttg aaatagatta
     6001 aagtagtagg ccatgcggac ttttgtgatg ccatctgatc acacctggca aaaacgatgg
     6061 cggtgcggcg catttcatgt tccaaactgt tcgcggtgtg cgttcctata tagacagtaa
     6121 cttgtgcgga gaataatgcc tctccggtag gttcttacgc ctaccttgtt ttctgcccac
     6181 aactggcata gtcttatagc tgatgacccc ctgggtttcc tcgtgtggtt cgagcggatg
     6241 acttatcgtt ccccaatttg aaaggaaagt acgcatgaag tggcaattgt ggcaatcggc
     6301 gttgcgacgt ttcatcgtaa ccggtgtcgt cggtttaacc gttctttgtg gcctttggta
     6361 ctttggcacg ggccttcaag ccgaggaggc attgccggca ccagcggctc ctgtccagcc
     6421 cccagccaag gacgtggaga tcaagccgct ggagggaacg ccgcaagagc tccttaaatt
     6481 cttggaagac ctcaacgctc agcaaggtcc tgccgatcca caggccatga tggagttcca
     6541 acgaaaacgg gcaaaggttt tcatagaggt tgcggacaag attctgttgc aggataagct
     6601 ccccgcggaa acgctggacg gggccttcca gttcaagttt cggggtttgt tggtcctgct
     6661 gcagatgggt gatacccagg cccgcgagtt attgcccaag ttggccgaac agttaatggc
     6721 caaggccgag gaaatcctta aggggacgcc gaccgaagac gctgcccaac gagccgtgca
     6781 atgggccatg atggcgccgc ggttgggcgg cccagacttt ttgccgaggg tgattgctct
     6841 ccccaaacga ttagaagagt tgggatttcg tcaactggcg acgcaacttc gtgggggatt
     6901 gctcatgctc cgtctgcgga tgggccaggc ggaagatcgg gcggaattgg ccaaggatgt
     6961 aacggccttt ctcgaggacg cttggacgcg tttcagtcag gccgagaaga tcactcatga
     7021 agatgtagcc atggtcgtgc ctgtgctcca atacctggaa ttcgcaagat tggatgtgaa
     7081 cctcagcgac tattacggcc ggtttggcgg tctcttcgcc aagaacgcgg atgagcaaat
     7141 tgcggaattg gggcagcaac ttcaaggaat tagtcgccgt cttggtctcg ttggcaagaa
     7201 aatggagctt gccgggaaga cggtggatgg caaagacttc gactggtctc aataccgagg
     7261 caagatcgtc ctggtagatt tctttgctac gtggtgtggg ccctgtcgag cggaaatgcc
     7321 taacgtcaaa gaaaattatg agcgtttcca ctccaagggt tttgatgtgg tggccatcag
     7381 catcgacgaa aaccgagctg atctcgaacg ataccttcag gaaaacaagc tgccctgggt
     7441 ggtgattcac aatcaggatc caaaggctca agggcctgcc aatccggcaa catactacgg
     7501 aataacggcc gtaccgaccg tcatgctagt tgacaaggac gggacagtcc tcacgtttga
     7561 tgcccgcggt ccgcagttgg gcaaaaagct agaagagctt cttggcccgc cggaacctcc
     7621 cgcaaaagat gaaaagcagg gaaccaccga gaagcagtga ttggcttgcg cagtgcatat
     7681 gagcgagccg cattaggccg agacaatcac ctggcggtca taaaccgcgt gtaaagacag
     7741 gccgcggtaa tcgcccattc gtggaagcaa gggtgggcaa tcacaagggt aatccctgcc
     7801 gggattccgt ctttcaaccg gcgttgggtc ccacggtctc caatttgacc aatggcagat
     7861 cgaatttggg gttttggctc aaaaagcgga cggggttgtt gtaaaagacc tcgagcgcct
     7921 cttggaggct atggccacgg cggcgaaatt caaaaatgca gtcgtggagc gtggccggat
     7981 cgctgggacc ccagtcggcc
//
LOCUS       DAOPTR010000027         7770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_7798_length_7770_cov_4.077900, whole genome shotgun sequence.
ACCESSION   DAOPTR010000027 DAOPTR010000000
VERSION     DAOPTR010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7770
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7798_length_7770_cov_4.077900"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            310..1308
                     /locus_tag="PKI05_01045"
     CDS             310..1308
                     /locus_tag="PKI05_01045"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HOA50809.1"
                     /translation="MADPGQRGFSPVQRILERYLPVWLVVLCTVAYFWPRLSGVPGDP
                     FLASKGYLKHMIAVTMFFIGSLLRPEELLDVFHRWPLVLFGTLVQYSSMPALAYLFGR
                     LMGLDGPFLIGVLMVGCVPGAMASNVLTLVARGNVSYSVSLTTSATLLSPLVVPFVMW
                     LTVGQFAEFPVVGTGIELLFMVVLPVGLGFTLSQLSRPWHKVGQRLGSIVANLVILWI
                     IAVVVAANRDTLQQITGPLVGALLGINVGGYLAGFLAGWLVRLPVPMRRALTLEIGMQ
                     NAGLGTTLVLALFPDQPQAAVPCALYTFGCMFTGTLLARTMAMFPAPGQGSQPEAA"
     gene            complement(1326..2579)
                     /locus_tag="PKI05_01050"
     CDS             complement(1326..2579)
                     /locus_tag="PKI05_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50810.1"
                     /translation="MDARFRPVAAGLIVLLLTSGASYPTPNFVIHCGDPQLAKELGDA
                     AENYRKELARLWLGKTLPDWSRPCPVTVHVGPNLGAGGATTFVFDRGEVYGWQMTIQG
                     SRERLLDSVLPHEITHMILASHFRQPLPRWADEGAATTVEHISEREKHRRMLLEFLQT
                     RRGISFRRMFAMEEYPRDIMPLYAQGYTLAEFLIQQGGHRRFIQFLETGLKDNQWEAA
                     LSQWYPYKDLSDLQTSWVTWVAQGYPQLRPLPNPMDSSPQQPVLASLENQWRPPQSSS
                     TSAESVYQRSATQAAWNASASERTGVIAASYDGGARASQGRSDATQAIYSGGVELDVN
                     GWRPAPLPEAMEGTLGPMAMASSPQTSAVPSSPGVTAPCSCGQTVPTVDTPANAVQFN
                     QVAHPRTAEPSSRILLDWNRDTPWR"
     gene            2854..3156
                     /locus_tag="PKI05_01055"
     CDS             2854..3156
                     /locus_tag="PKI05_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50811.1"
                     /translation="MQAREAAQVNQVSMEERSRMLRPQLRVYYGPQEDEQLEQSPHSG
                     VTSVVNVPLREVLPLLVDAVRSKRTWLKDFENEEITLSLDLYEVLLAYQHYRRPSA"
     gene            3735..4511
                     /gene="tpiA"
                     /locus_tag="PKI05_01060"
     CDS             3735..4511
                     /gene="tpiA"
                     /locus_tag="PKI05_01060"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOA50812.1"
                     /translation="MRRPFVAGNWKMNMERASAVALARAVADGARAYPQVDIAVCPPF
                     VYLEAVAQAIAGSPVALGAQNLYPEPKGAFTGEISGPMLKDMGCTFVILGHSERRAMG
                     ESSEFVNEKVFAALECGLKPIICVGETLRQRKEGRTLTVVRRQIDGSLSGLSAEQMRQ
                     CVIAYEPVWAIGTGENATPDQAEEVQAEIRRLLAERFGKDVADSVRIQYGGSVKASVA
                     ADIFEKPNVDGGLIGGASLKADEFLGIVAAAQKVKGRNGG"
     gene            4536..4949
                     /gene="secG"
                     /locus_tag="PKI05_01065"
     CDS             4536..4949
                     /gene="secG"
                     /locus_tag="PKI05_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOA50813.1"
                     /translation="MKFVFLILLVVTSLFLILLVLIQRGRGGGLAGAFGGFGGQSAFG
                     AKAGDTFTRITIGVAAFWIGLCIFTLLYFRWTHRSRLDLGPTGPSVTNTAPLAPTTLP
                     PAPGGEGSGASGGSSGTTPSGGTGQTGSGTQESGS"
     gene            5091..5984
                     /locus_tag="PKI05_01070"
     CDS             5091..5984
                     /locus_tag="PKI05_01070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOA50814.1"
                     /translation="MLLSMTGFGEARREHEGFSVRVEIRTVNARYLKISIRSNNAIPG
                     GLENEVEALIRRAIRRGTVNVGVFVDHEPQAGDYRINGQVLLGYLKQVSNLAVDHGLV
                     PPTTLDSFLLLPGVVSDLSDPAAQSERIRPVVIAAVEEALDRLQAMRRAEGHAMTEDL
                     RANCQRILELVERVEARAPQVVEDYRQRLQERMNQLLGPAAASLRPEDILKEASFFAE
                     RSDISEELVRLRSHVQQFQEILTKGGGEAPGRRLEFLTQEMVREVNTMGAKANDVEIS
                     HHVVEMKTLVERIREMVQNVE"
     gene            5997..6599
                     /gene="gmk"
                     /locus_tag="PKI05_01075"
     CDS             5997..6599
                     /gene="gmk"
                     /locus_tag="PKI05_01075"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOA50815.1"
                     /translation="MSGQLPGRLVILSGPSGVGKTTVANRVLADSPVPMVRSVSVTTR
                     PRRPDEIDGVHYRFVSPEEFERLRAQQAFLECFQVFGNGHWYGTLRQAVLSALSAGKW
                     VLLVIDVNGALAVQEEFPDAVSIFLLPKDWQSLEERLRGRGTEPEEVVRLRLERAAEE
                     LRLAEKYRYRVVNDDLEQTVRDIIEILKKEAKEASRPETS"
     gene            6707..6967
                     /locus_tag="PKI05_01080"
     CDS             6707..6967
                     /locus_tag="PKI05_01080"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOA50816.1"
                     /translation="MYDELRDDRLIDKVGGRFKLSTLIQKRLVALMKGAPPLVDLDTK
                     DRMKIVLREILEDKIYLDLGDEVRTVADLPEPKSTVRDLRSL"
     gene            6964..7512
                     /locus_tag="PKI05_01085"
     CDS             6964..7512
                     /locus_tag="PKI05_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HOA50817.1"
                     /translation="MKGHQILIGVTGGIAAYKTAALVSKLVQAGAGVTVVMTQAATQL
                     IGPKTFEALTGRPVAVDLWAPIMSHAHIELADRGEVFCIAPATANFLAKAAWGIGDDL
                     LTTTVLAFSGPIVVAPAMNSRMWQKPAVQRNLRQLREDGFIIIEPGEGYLSCGMVGPG
                     RMAEPEEIFARLGELLKGNKTA"
BASE COUNT         1568 a         1992 c         2472 g         1738 t
ORIGIN      
        1 gagggttggg gtgagggcaa gcgaacgtca gaatcgtttg agaaaaccag cagcgattcg
       61 agtcaccctc acccccgacc cctctcccgc cgaacgggcg aggggaggtt atggaatagg
      121 ctcttattga aaggcccagg atagggcctc ttttttttcg gaaaactctc aaaaacggac
      181 ggcggtgccg actgagccat tttttccatt ttcgtgggtc cgtttcttta tctgacatgt
      241 tacaatcggc gctgttggtc gcctgcccag tcttcttcgg agttgactac ctgcaaaagg
      301 acgatcaagg tggcggaccc aggtcaacgc ggtttttctc cggtacagcg catcctcgaa
      361 cgttatctgc ctgtttggct tgtcgttttg tgtacggtgg cttacttttg gccaagactc
      421 agcggcgtgc cgggagaccc gtttttggcg agcaagggtt acctgaagca tatgatcgcc
      481 gtcacgatgt tcttcatcgg ttcactcttg cgcccagaag aactgttgga tgtctttcat
      541 cgctggccgt tggtcctctt cggaacgttg gtccaatatt cgtccatgcc ggcgttggcc
      601 tacctttttg gacgactgat gggactcgac gggccgttct tgattggcgt tctcatggtc
      661 ggttgtgttc ccggggcgat ggcctctaac gtcctgaccc ttgtcgcccg ggggaatgtc
      721 agttactcag tcagcctcac cacgagtgcc acgctccttt cgcccttggt cgttcccttt
      781 gtgatgtggc tgacggtggg acaatttgct gagtttcccg tggtgggcac cggcatcgaa
      841 ctccttttca tggtggtctt gcccgtggga ctgggattca cgctctccca actttcgagg
      901 ccttggcaca aggtgggcca acggttgggc tcaattgtgg ccaatctggt gatcttgtgg
      961 attatcgcgg ttgttgtggc ggccaatcgg gacacgcttc aacaaatcac gggaccctta
     1021 gtgggagcgc ttttgggtat caacgtcggt ggatatctgg ccggcttctt ggccggttgg
     1081 ttggtgcggc ttccggtgcc gatgcggcgg gccttaacac tggagatcgg aatgcaaaat
     1141 gccggattgg ggacgacact ggtgctggcc ctctttcccg accaacctca agcagccgtg
     1201 ccctgtgccc tttacacctt tgggtgtatg tttacgggaa cacttcttgc gcgaactatg
     1261 gcgatgtttc ccgcacctgg ccaagggtct cagcccgaag cggcataacg tttatgagtg
     1321 cctgctcatc gccacggagt gtcgcggttc caatcgagga gaatgcgact ggaaggttcg
     1381 gctgtccgcg ggtgggccac ctggttgaat tggacggcat tcgcgggagt gtcaaccgtt
     1441 ggaaccgttt ggccacatga gcacggggcc gtcacgccgg gtgacgaagg aaccgcggag
     1501 gtctgagggg aagaggccat cgccattggt ccgagtgtgc cctccatcgc ctcgggaagc
     1561 ggggcgggac gccaaccgtt gacgtccaat tccacgccgc cggagtaaat ggcctgcgtc
     1621 gcatcggaac gcccctgaga agctcgggcc ccaccatcat atgaggccgc aatcacacca
     1681 gtacgctcgg aggctgaggc attccaagcg gcttgggtag ccgatcgttg ataaacggat
     1741 tcggccgatg ttgaggacga ttggggcggg cgccattgat tctccagcga agcgagcacc
     1801 ggctgctggg gagaactgtc catcgggttg ggcagggggc gaagttgtgg gtaaccctgg
     1861 gccacccagg tgacccaact cgtttgcaag tcgctgaggt ccttgtaggg gtaccattgg
     1921 ctcaaggcag cctcccattg gttgtcctta agcccggttt ctaggaattg gatgaaccgt
     1981 cgatgcccac cctgttggat gaggaactcg gccagcgtat agccctgggc gtacaacggc
     2041 atgatgtccc gggggtactc ctccatggcg aacatccggc ggaaggagat accgcgccgc
     2101 gtttgcagaa attcgaggag cattcggcga tgcttttcgc gttcactgat atgctccacg
     2161 gtggtggcgg cgccttcgtc ggcccaacgg ggaagcggct ggcggaaatg actggccaag
     2221 atcatgtggg taatctcgtg cggcagaacg gaatccagca gtcgttcgcg ggaaccctga
     2281 atggtcattt gccagccgta aacctcgccg cgatcgaaga cgaatgtcgt ggctccgccc
     2341 gccccaagat tcggccccac atggaccgta acggggcatg gtcgtgacca gtctgggagc
     2401 gttttgccaa gccaaaggcg ggccaactcc ttccggtagt tctcggcggc gtcgcccaat
     2461 tctttcgcca actgcggatc gccacaatgg atgacaaaat tgggggtggg atagctcgcc
     2521 ccactcgtca aaagaaggac gataagccct gcggcaacgg gtcgaaaacg agcgtccatg
     2581 ctgattgcga cttccttgtc taggtcgagg ggtcatcatc cttgggaatt gagacccctc
     2641 actatcgtta acggaaatac gcagccttca cgccgtgtaa ctgaggtccg cgtcaattta
     2701 ccacgcccga caaaacccgc aaagcccgat ttctggttga aacccaaaat cccgtaaaaa
     2761 tcgttaccga aggaaaaatt ttccaaaggt tcggtcgagg gtggtcgata atcaatcgcg
     2821 cccaggggag tggtttgtgt ccatggaaca gttgtgcagg caagggaggc cgcacaggtc
     2881 aaccaggtaa gcatggaaga gaggtcgcgg atgcttcggc cccaattgcg tgtgtattat
     2941 ggtccccagg aagacgaaca gttggagcag tccccccatt ctggggtgac ttctgttgtg
     3001 aatgttcccc ttcgagaagt gctccccctt ttagttgacg ccgtccggtc aaagcgaacg
     3061 tggttgaaag attttgagaa cgaagagatt acgctttccc tcgatctgta cgaggtcctg
     3121 ctggcttacc agcattaccg ccgcccatca gcgtgatacc caccagcgag gggaaataac
     3181 ctccacgaag gatggcaaca agcctttcta ccggcgaagc cggtaaagag gacaaacccc
     3241 atcacaagat tgcggtagcc atttttatcg cctggacatg ttccaatcct tgtaggggca
     3301 acccttgtgg ttgccccagc gtttttgcgc acggcgcgca ccaaatgaaa gcgaagtcga
     3361 ggagccggcg gcaaggatct gacgcgcagc ccgcccctag gaatctatgg gaaatgcata
     3421 aagggcgaaa acgtacccca cctcgcggtg ggactcaggc tgtttgcctc ggccacgaga
     3481 gtagtgggcc ccaccgaatc gcgcgggcaa ggtctgcccc ttgtcaaaaa cagggatatg
     3541 ggccaagatg agggttttcg gtcgcgctcg gggattgcgc cgattaagcc cgtcgtccga
     3601 gaactgtcat aacacctggg aggctgaatc tctcggttct gatggttggc cattggcggg
     3661 ctgtctcttg aaattggttt cgtattacct ccagtccgga caacaggttg ggtgaggaaa
     3721 aggagaaaag cactatgcgg cggccatttg ttgccggtaa ttggaagatg aatatggaac
     3781 gcgcctcagc cgtagcattg gcccgtgccg ttgccgacgg tgcacgtgct tatccgcaag
     3841 tggatattgc cgtctgcccg ccgtttgtct atctcgaggc cgttgcccag gccatcgccg
     3901 gaagcccggt cgcccttggt gcccaaaact tgtatcccga accgaagggt gcgtttaccg
     3961 gtgagatcag cgggccgatg ctcaaggaca tggggtgtac gttcgtcatt ctgggccaca
     4021 gcgagcggcg tgctatgggc gaatcgagcg aattcgtgaa tgaaaaggtc ttcgccgctc
     4081 tcgagtgcgg cctaaagccc atcatttgcg tgggggaaac gctccgtcaa agaaaggaag
     4141 gccgaacact aacggtagtg cgccgtcaga ttgatggcag tttgtcgggg ctgtcggccg
     4201 agcagatgcg gcaatgtgtg atcgcttacg aaccggtatg ggcgattggg acgggtgaga
     4261 atgccactcc cgatcaggcc gaagaggtgc aggcggagat tcgtcgtttg cttgccgagc
     4321 gatttgggaa ggatgttgcg gattcggtga gaattcagta cggtggaagt gtgaaggcct
     4381 cggtagccgc ggatatcttt gaaaagccca atgtggatgg cggcttgatt ggtggggcca
     4441 gcctgaaagc ggatgagttt ctcggtattg tggcagcggc gcaaaaggtc aaaggccgga
     4501 acggcggttg atacgctgag gatcggaata ggattatgaa atttgtcttt ttgatcttgc
     4561 tggtggtcac ctcgctgttt ttgatcctgc tggtcctaat ccagcggggt cgcggcggtg
     4621 gattggcggg cgcatttggc ggctttggcg gtcaaagtgc ctttggtgca aaggccggtg
     4681 atacgtttac gcggatcaca attggcgtag ctgcgttttg gattggcttg tgcatcttta
     4741 cattgctcta tttccgctgg acacaccgga gtcgattgga ccttggcccc accggacctt
     4801 ctgtcacgaa taccgcgccg cttgcgccaa cgacgttgcc gccggcccca ggtggagagg
     4861 gttccggggc aagcggaggg agctcgggga cgacgccttc cggtgggaca gggcaaaccg
     4921 gttcgggcac acaagagagc ggttcgtaaa gcgagctgct caggggagga acgcctaagt
     4981 tccatcggga tgttcaggtg cggagcggtt gtccagcgga accggcgtcg ctagatgagc
     5041 ggggcatggt gagtgccttt cattgagagg agaacgctac ggaggcagcc ttgctcctga
     5101 gcatgactgg ttttggcgaa gcccgcaggg agcacgaagg tttctccgtg cgggtggaaa
     5161 tccgtaccgt caatgcccgc tatctcaaga tcagcattcg cagtaataat gcgatacccg
     5221 gcggtttgga aaatgaagtc gaggccttga ttcgccgggc cattcgccgg ggaaccgtga
     5281 acgtcggtgt gtttgtggac catgagccac aggccggcga ttaccggatt aatggtcaag
     5341 tgcttttggg gtaccttaaa caggtgtcga acttggctgt ggatcatggg ctagttccgc
     5401 cgacaacttt ggactcgttt ctccttttgc cgggggttgt gagtgatctg tctgatccgg
     5461 ctgcccaatc ggagcgaatt cgcccggtgg taattgcggc tgtggaggag gccctggaca
     5521 ggcttcaggc gatgcggcgg gcagaaggtc atgccatgac ggaggacctc cgcgcgaact
     5581 gccagcggat tttggagttg gtggaacgtg tggaagcccg tgctccccaa gttgttgaag
     5641 actatcggca gcgtctgcaa gagcggatga atcaactgct cggtccggcg gctgcgagcc
     5701 tgaggccgga ggatatcttg aaggaggcaa gtttctttgc cgaacgctcg gacatttccg
     5761 aagaattggt tcgcctgcgg agccacgtgc aacagttcca agagatttta accaagggcg
     5821 gaggggaggc cccaggtcgt cggctggagt ttctcaccca ggagatggtt cgcgaagtca
     5881 acaccatggg ggcgaaggcc aatgatgtcg agatcagcca tcatgttgtg gagatgaaga
     5941 ccctcgtgga gcggattcgc gagatggtcc aaaatgttga gtgacggggc aaagcgatga
     6001 gtggacaatt gccaggacgt ctggtgattc tgtcgggtcc ttccggtgtg ggaaagacga
     6061 cagtggctaa tcgcgtcctg gccgattccc ctgtgcccat ggttcgaagt gtgtcggtaa
     6121 cgacgcgacc gcgccgaccc gacgaaattg atggtgtcca ctatcgtttt gtaagccccg
     6181 aggagttcga gcggctgcga gctcagcagg ccttcctaga gtgctttcag gtatttggca
     6241 acggacattg gtatgggacg ctgcggcaag ctgtgctctc ggcgttgtcg gcggggaaat
     6301 gggtcctttt ggtaatcgat gtcaacgggg ccttggccgt ccaggaggaa ttccccgatg
     6361 ctgtgagtat tttcctgctg ccgaaggatt ggcagtcctt ggaagagcgt ctccgcggtc
     6421 gggggacgga acccgaggaa gtcgttcggc ttcggttgga gcgggccgcg gaggaattgc
     6481 gattggcgga aaagtaccgg tatcgcgtgg tgaatgacga tttggagcaa accgttcggg
     6541 atatcatcga gattctgaag aaagaagcga aggaagcatc ccggccggaa acgtcttgag
     6601 ggagacaaga caaaaaagag tgcgttgaat tttcaaggag acatcaaacg cgttgtgcga
     6661 cgaacctgcc cacaacgttg gtcctattat cggaggttga gcgaagatgt acgacgaact
     6721 gcgagacgac cgattgattg acaaagttgg tggtcgtttc aaattatcga cgttgatcca
     6781 gaagcggttg gtggcgctaa tgaagggcgc cccaccgttg gtggatttgg ataccaaaga
     6841 ccggatgaag attgtgttgc gtgagattct tgaggacaag atttatctgg atctcggcga
     6901 tgaggtccga acggttgccg accttcccga accgaaatcc accgtccgcg atttacgctc
     6961 gttatgaaag ggcatcagat ccttatcggc gtgacggggg gcattgccgc ctacaagacg
     7021 gccgccctgg tcagcaagct ggtccaggca ggggcgggcg tgactgtggt gatgacacag
     7081 gcggcgaccc agcttatcgg ccccaagacc ttcgaggcct tgacagggcg gcctgtggcg
     7141 gtggatcttt gggcccccat aatgtctcat gcccacatcg agttggcaga tcggggtgag
     7201 gtcttttgca ttgccccggc aacggccaat tttttagcga aggcggcttg gggcattggc
     7261 gacgatttgt tgacgaccac cgttctggca ttttctgggc cgattgtggt cgctccggcc
     7321 atgaatagcc gcatgtggca gaagcccgcc gttcagcgga acctccgcca gcttcgtgag
     7381 gatggtttca tcatcatcga gcctggcgaa ggttatctca gttgcgggat ggtgggcccc
     7441 ggtcggatgg ccgagccgga ggaaattttc gcccgccttg gtgaacttct caaggggaac
     7501 aaaactgctt gagggaccgg cctaacccat cgcttgggcc tatcccacaa cctcccctcg
     7561 cccgttcggc gggagagggg tcgggggtgg cttgaatggc tgctggtttt ctcaatcgat
     7621 tccgacgttc actcgccctc accctaaccc tctcccaaag ggagagggtg tttcaggata
     7681 gggttttagg cgaaaggatt atatacgccc ggcatcggaa tggggtaatt accttcggca
     7741 tctttggtaa caggggctgg ggcatcccaa
//
LOCUS       DAOPTR010000028         7698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_7889_length_7698_cov_3.440403, whole genome shotgun sequence.
ACCESSION   DAOPTR010000028 DAOPTR010000000
VERSION     DAOPTR010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7698
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7889_length_7698_cov_3.440403"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /locus_tag="PKI05_01090"
     CDS             <1..1316
                     /locus_tag="PKI05_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50818.1"
                     /translation="MGAWRRPGLIDAFRRKLINGMLANGFTPEYAEAIFRQIRGFGDY
                     GFPESHAASFALLVYVSAWLKYHYQAAFTVGLLNSQPMGFYAPAQLITDAKRHGVEIR
                     PVDINYSYWETDLEGATAFPVVSSERDSSWGRCPENVESLSEDGGSLFPFSEDENSQW
                     HSSPVPDDILRSGSKHSFPESSLLPAKAIRLGFHMVHGLRESDVEKIIEARKDGPFTS
                     IADVMRRTGLSQATIIRLARADAFGSLGLRRREALWEARALSTATPPPLFQDRTSSDQ
                     SLAGGISSSADTREQSHSPPSMNLAEEVLADYAMIGLSLKAHPLRLLRKELNQLQVVP
                     AERLLEIPDGHPVRVAGMVLVRQRPATAHGITFMTIEDETGIVNLVVRPDVWQRWRSA
                     ALNATLLFAVGRLQKQNGVIHVVAIRLEDLSAKLAGLQARSRDFC"
     gene            complement(1423..3354)
                     /locus_tag="PKI05_01095"
     CDS             complement(1423..3354)
                     /locus_tag="PKI05_01095"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOA50819.1"
                     /translation="MTRILAAIFCLITIIAGRLCVAEDVSTLTDSAKLVAILESKESS
                     DFEKAVACKRLAVFGTAEAVPVLAKMLLDEKFSHYARYALEPIPAAVVDDALREALSR
                     AEGGVRLGILNSIGVRRDTKAVVAIAPLLNHDEPAVVDAAASALGRIANVEAADILKK
                     SLQSEKATTRRAVGDALLICAEIVKKEGNTELAIDIFDAVRKADVPKPVRLAAMRGAV
                     MARGDNGVTLLKELLETDDSGVFDLALRVAREMKSAAATEELVKHLGVVSEDRQAKIV
                     EALADAGVQEALPAIRKAVAEASGAMKLAAIRSLAKCGDKSDVALLLEVAKSSDEVVA
                     EQAKAALVSLQGPGVGEALADSLAKEDSKGKLVLLEVVGQRRTLAASPVVRQLAASAD
                     PSIRLAAIRTLGNLGQDEDVAMLVERLITSPSADDVAAVKDALQAASQRVRDRDAIAK
                     MLIQHMDKAPGNTRRDLLEVLGSLGGPTALAAVAGAAQSQDSSLQDAATAILGNWLSP
                     DVAPVLWDLIQKNAAPQYRIRLIRGYIRVARQFNVPDAERLAMCRNALQVSDRPDERR
                     LVMDVLRRNPSAEGLALAVAEIDRPGMRAAACETIVTIAEKLQKNVPGLREALSQVVQ
                     KATDPAIKARAQAILERLP"
     gene            complement(3402..4676)
                     /locus_tag="PKI05_01100"
     CDS             complement(3402..4676)
                     /locus_tag="PKI05_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA50820.1"
                     /translation="MRHVTKICRRRFLGTSIGALSALAVPSLIPGKALGLNGFVAPSE
                     RIVMGGLGIGPRGRTDLAAFLKNPDVQFVAVADAQAARREAVKQMVDRHHQNSDCKTY
                     RNMFDLLLRDDIDAVLIATGDRWHTMASIYAAKAGKDIYCEKPCSMTIAESRALAETI
                     RRYGRVYQAGTQRRNIGNFIFAINLVYSGKLGKLHTVHANTLDPRTTHEWLPAEPEPP
                     KDECDWDLWLGPCPWRPFNQRYVNGGWRGHFDFHGGGILEWGSHTVDLCQWANKADNT
                     APIEFEPQGNHVHATYANGVKLIMRDTGWLGLGTCSVRFEGDEGWIETGDSGQVAVYP
                     ESLRSEMPVFTMAGTDPSTHIRDFLNCVKSRAMPAANADVAAQSHIACHAAYIAWQLG
                     RKLRFDPVKEEFIGDDEANRMRSRALREPWHI"
     gene            complement(4734..5783)
                     /locus_tag="PKI05_01105"
     CDS             complement(4734..5783)
                     /locus_tag="PKI05_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOA50821.1"
                     /translation="MRVHQMASFRGVFGLAIALLLASGWSFYAFADEDGPWVVYRGQQ
                     GPGLGKHIVLISGDEEYRSEEALPMLGKILAKWHGFTCTVLFAIDPATGEIAPNVRDN
                     IPGLEALENADLMIIATRFRDLPDEQMSHIVNYLKKGRPVMGFRTATHAFAIPAGKTY
                     AHMSWDCKEPGWEGGFGRIVLGETWIAHHGAHNKESTRGVIAPEMKDHPIVRGCDDIW
                     GPTDVYRVRLPLPGDSQPLVLGAVLEGMSPQDKPVEGPKNDPMMPVAWIKTYTWENGP
                     AGRVFTTTMGAAVDFKSEGLRRLVVNAVYWCLGMEDLIPKKANVEIVGQFEPNYFGFN
                     GYKKGLRPSDFAGLD"
     gene            complement(5917..7164)
                     /locus_tag="PKI05_01110"
     CDS             complement(5917..7164)
                     /locus_tag="PKI05_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA50822.1"
                     /translation="METIRYGIVGGGFYAKFQLRALEQVRGIEVAGLVSRRPPEALSQ
                     FVQAKRLGEGRIFPSVREMAHHVDVVAIYAPNFARVPIVEEIGKALDEGAKLRAVICD
                     KPLARNLREARQMIERLRPFGVPLIYFENQLHMKAVQQARAQLEPVVKTMGPLTLVRA
                     SEEHAGPHSAWFWDPVQQGGGVMTDMGCHSVAVAWYLLTPPGKPLGFLRPIAVQADLS
                     LLKWGRPQYREALRQRFGVDYSKTPADDFATGLITFLNPETNCISKAQFTVSWMYDKQ
                     GLRVMIDGIGPGYAFEFNTLRSPLEVFVGDVAAAAVADAEAALEKQQATRGLVTVQPN
                     EPDLYGYVDENIDALESLLTGRPALMDWDFGREIVKLTMAAYLSAERRATVDLTDPQT
                     LAYLEDYVPLIQQGRGREVLHIP"
BASE COUNT         1661 a         2280 c         2019 g         1738 t
ORIGIN      
        1 ccatgggagc gtggagacgc cctggcttga tcgacgcttt tcgacgcaaa ctcatcaacg
       61 gcatgttggc caatggattt acgcccgaat acgccgaggc catctttcgg caaatccgcg
      121 gctttggtga ttatggtttt ccggaatccc atgccgccag tttcgctttg ctcgtctatg
      181 tttcagcctg gttgaaatat cattaccagg cggcctttac cgtaggcctt ctgaatagcc
      241 aacccatggg attttatgcg ccagctcagc tcatcactga tgccaaacgg catggggtgg
      301 aaattcggcc cgtcgatatc aactacagtt attgggaaac tgacctagag ggcgcaactg
      361 ctttccctgt tgtaagttca gagcgtgaca gctcttgggg cagatgccct gaaaatgttg
      421 agtcgttaag cgaagacgga ggatcattgt tcccattttc agaggacgaa aacagtcaat
      481 ggcattcctc gcctgttccc gatgatatcc ttcgctctgg tagtaagcat tcgtttcccg
      541 agtcctctct tttaccagcc aaggccattc gccttgggtt tcacatggtt catggattac
      601 gagaatcgga tgtcgaaaaa atcattgaag cccggaagga tggccctttc acaagtattg
      661 ccgatgtaat gagacgaact ggtctaagtc aagccacaat cattcgtctg gcccgcgccg
      721 acgcttttgg ctcgctgggt ttgcggcgac gggaagccct ctgggaagcc cgtgcccttt
      781 caaccgccac acctcctcct ttgtttcaag ataggacctc ctcggaccag agtttggccg
      841 ggggtatttc ctcgtcggcg gatacccggg agcaaagtca ttctccgcct tccatgaacc
      901 tggcggagga agttctggcg gattatgcca tgatcgggtt gtctctaaag gcccatcccc
      961 ttcgacttct tcgaaaagag ctaaatcaac tccaggttgt ccccgccgag cgattgctgg
     1021 aaattcctga cggccatcca gttcgcgtgg cgggaatggt tctagtgaga cagcggccag
     1081 ctacggccca tgggatcact tttatgacca tcgaagatga gaccggcatt gtgaatcttg
     1141 tcgtccgccc agatgtgtgg caacgctggc gatcggcggc ccttaatgcc acacttcttt
     1201 ttgcagtggg cagacttcaa aaacaaaacg gcgtcattca tgtggtcgct atccgattag
     1261 aggacctttc tgcaaaactg gccggtctcc aggcaagatc gcgagatttt tgctgacacc
     1321 cactttttgg gcacaaaaaa ccggtattcg aaatttccgt cccgaatacc ggtttgctcg
     1381 gcagaatcat ttaattccgt aacggatagc tatccgatct gattacggaa gcctttcgag
     1441 gattgcctgg gcacgggcct tgatcgccgg atccgttgcc ttctgcacga cttgtgaaag
     1501 ggcctcccgc aacccgggaa cgttcttttg cagtttctcg gcgatcgtca caattgtttc
     1561 gcaggcagct gcccgcatcc ctggtcgatc gatctcggcc actgcgagcg ccaacccttc
     1621 cgccgaggga ttccgccgca aaacatccat gaccaagcga cgttcatccg gccggtcaga
     1681 aacttgcagg gcattacggc acatggccaa ccgttctgcg tcaggcacat tgaactgccg
     1741 agcgacccga atgtaacccc ggatcaagcg aatccggtac tggggcgccg catttttctg
     1801 aatgaggtcc caaagaacgg gtgcaacatc cggtgacagc cagttaccca aaatagctgt
     1861 tgccgcatct tgcagactgg aatcctggct ctgagcggcc cctgccacgg cagcaagtgc
     1921 cgtcggccca ccgaggctcc cgagaacctc cagcaagtcc cgccgcgtat tacctggggc
     1981 cttatccatg tgttgaatca gcatcttggc gatagcatct cgatcccgta cccgctggct
     2041 cgccgcctga agagcatcct tcacggcagc aacgtcgtca gcggatggcg acgttatcaa
     2101 gcgttccacc agcatcgcga cgtcttcatc ttggccgagg tttcccaacg tgcggatcgc
     2161 cgccaggcgg atactcggat cagcgctggc agccaactgg cggacaacgg gagaggccgc
     2221 caacgtccga cgctgcccga caacttccaa gagcacaagt tttccctttg agtcttcctt
     2281 ggcaagggag tcggcaaggg cctccccaac tcccgggcct tgcaaagaca cgagggccgc
     2341 cttagcttgc tcggctacta cctcatccga ggactttgcc acttcgagta aaagcgccac
     2401 atcggacttg tcaccgcatt tcgcgagact tcgtatcgct gccaacttca ttgcgccgct
     2461 tgcctcggcc acagccttgc gaatcgcagg cagcgcttcc tggacaccgg catctgccag
     2521 ggcttcaaca attttggcct gcctatcttc ggaaaccaca ccgagatgtt tgaccagttc
     2581 ttccgttgca gcggcagact tcatttctct tgccacacgg agggcaaggt cgaatacgcc
     2641 actgtcgtcc gtctccagga gctctttcaa taaggtcacc ccgttgtcac cccttgccat
     2701 cacggcaccc cgcatcgccg ccagccgaac gggtttggga acatccgcct tcctgaccgc
     2761 atcgaagatg tcgatggcaa gttccgtgtt tccttctttc ttgacgattt ccgcgcagat
     2821 taaaagggca tccccgacgg cgcgtcgtgt tgtggccttt tcactttgaa gagacttttt
     2881 cagaatgtct gcggcttcta cgtttgcaat tcgccctaat gccgatgccg cggcatcgac
     2941 cacggcaggc tcgtcatgat ttaacaacgg tgcaatcgcc acaactgcct tggtgtcacg
     3001 ccgtacaccg atcgagttta agattcctag acgcaccccg ccctcggctc gcgaaagggc
     3061 ttcccgaagc gcgtcgtcaa ccaccgccgc ggggatcggc tcaagggcat atcgggcata
     3121 gtgggagaac ttttcatcca aaagcatctt agccaggacg ggaacggcct cggccgtgcc
     3181 gaaaaccgcc agacgtttgc aggccaccgc cttctcaaag tccgaggatt ctttggactc
     3241 caagatggcg accagtttcg cactgtcggt gagggttgaa acatcctccg cgacgcataa
     3301 ccgccccgcg atgatcgtta tcagacagaa gatggcagcg agtattcgag tcatgattgt
     3361 cctcctatca gcgatcggtt gatgatactc gtaagggagt gttaaatatg ccacggctcc
     3421 cgcaaggccc gggaacgcat ccggtttgct tcatcgtccc cgatgaactc ttccttcacg
     3481 ggatcaaagc gaagcttgcg gcccaattgc caggcaatgt aggcggcgtg gcaagcgatg
     3541 tgcgactgtg ccgcgacgtc agcattggcg gccggcatag cccggctttt gacgcaattg
     3601 agaaagtcgc gaatgtgcgt ggaaggatct gtgcccgcca tggtaaagac cggcatttct
     3661 gagcggagcg attccggata aaccgcaacc tggccactgt cgccggtctc gatccagccc
     3721 tcgtcacctt caaatcgcac actacacgtg cccaaaccca accaaccggt atcgcgcatg
     3781 atcaacttca caccgttggc gtaagttgcg tggacatgat tgccctgtgg ttcaaactca
     3841 atgggtgccg tgttatcggc tttgttggcc cattggcaca gatccacggt gtgcgatccc
     3901 cattccagaa tcccgccgcc gtggaaatca aaatgccccc gccatccacc attgacatag
     3961 cgttgattga aaggccgcca gggacaaggt cccagccaca aatcccagtc gcactcgtct
     4021 ttggggggtt ccggttccgc aggtagccat tcgtgggtgg ttcttggatc aagcgtattc
     4081 gcatggaccg tatgcagctt gcccaacttc ccactgtata cgagattgat tgcgaatatg
     4141 aagttaccga tgtttcgccg ctgggttcct gcttgataca cccgcccgta gcgccggatc
     4201 gtttccgcca gggcgcgact ttcggcgatg gtcatggagc atggcttttc gcaataaatg
     4261 tcctttccag ctttggcagc gtagatggat gccatggtgt gccatcgatc gccggtagcg
     4321 atcaatacgg cgtcgatgtc atcccgcagc aagagatcaa acatgttgcg ataggtcttg
     4381 cagtcggagt tctgatgatg ccggtcgacc atctgtttga cggcttcccg ccgtgccgct
     4441 tgggcgtcgg ccacggctac gaattgcacg tcgggattct tcaggaacgc ggccaggtct
     4501 gtccgaccac gcggcccaat ccccaggccg cccatgacaa tccgctcact gggcgctacg
     4561 aagccattta atcccaaggc ctttcccggg atgagggaag gaaccgctaa ggccgacagc
     4621 gcaccgatgc tcgtccccag aaagcgacgg cgacatatct ttgtcacgtg tctcatcgat
     4681 taacctcctt ttacttggtg gggttgcttt cagaaaaaca gggttccctt cgttcagtcc
     4741 aagccggcaa agtcactggg gcgcagaccc ttcttataac cgttgaagcc aaaatagttt
     4801 ggctcgaatt ggcccacgat ctccacgttg gccttcttgg gaatcagatc ctccattccg
     4861 aggcaccaat aaacggcatt aaccaccaat cgccggagtc cctcgctctt gaaatcgact
     4921 gccgctccca tggtggttgt aaagacgcgc ccggccggac cattctccca tgtgtaggtc
     4981 ttgatccacg ccaccggcat cataggatcg ttcttcgggc cctcaaccgg cttatcttgc
     5041 ggcgacatcc cttcgagaac ggcaccaagg accaaaggct gggaatcccc cggcaaagga
     5101 aggcgaacgc gataaacgtc ggttggtccc caaatgtcgt cacagccacg aacgatggga
     5161 tggtctttca tctcgggcgc aatgacgccg cgggtgcttt ccttgttgtg ggcaccatga
     5221 tgggcaatcc atgtctcccc gaggacgatg cgaccgaagc cgccttccca accgggctct
     5281 ttacaatccc agctcatatg cgcatatgtt tttccagccg gtatggcaaa ggcgtgagtc
     5341 gctgtgcgaa atcccataac cggccgacct tttttcaggt aattcacgat gtggctcatt
     5401 tgctcgtcgg gcaaatcccg aaagcgggtc gcaataatca tcaagtcggc gttttcaagg
     5461 gcctcaagtc cagggatatt gtcccgaacg tttggggcaa tttcaccagt ggcggggtcg
     5521 atggcaaaca acactgtgca ggtaaaccca tgccactttg ccaagatttt gcccaacatg
     5581 gggagggcct cttccgagcg atactcttcg tcgcccgata tcagaacgat atgcttgccg
     5641 agaccaggcc cttgctgccc ccgataaaca acccagggac cgtcttcgtc ggcaaaggcg
     5701 taaaatgacc acccactggc caatagcaag gcaatagcca gtccgaaaac acccctaaag
     5761 gaggccattt ggtgcacacg catggttgtt ttctccgttt tcgtcaccca aaacctattc
     5821 aaatcagttc ttagcaaata gtgtaaacac cacttaccca tactcaaatg gtgatggcta
     5881 agcacagcgc acggacattt ccttcaattg ccgtcattac gggatatgaa gtacctcccg
     5941 tccgcggccc tgctgaatga gaggcacgta gtcttctaga taggcgagcg tttgcgggtc
     6001 cgtcaaatcg acggttgctc gccgttccgc cgacagatag gccgccatcg tcaatttcac
     6061 aatttctcta ccgaagtccc agtccatcag ggccggacga cccgtcagca aggactccag
     6121 agcgtcgatg ttttcgtcca cgtaaccata cagatcgggt tcgttaggtt ggacggtgac
     6181 caacccgcgc gttgcttgct gtttttccaa ggcggcttcg gcatcggcaa cagcggcggc
     6241 cgccacgtca cccacgaaga cttccagtgg cgaacggagt gtattgaact cgaaggcata
     6301 accgggtcca atcccgtcga tcatgacccg gagcccttgc ttatcgtaca tccacgaaac
     6361 tgtgaactgt gccttgctga tgcaattggt ttcaggattc aagaaggtga tcaaccccgt
     6421 ggcgaagtcg tccgcaggag tcttggagta gtcaacgccg aatcgctgtc gaagtgcttc
     6481 acggtattga gggcggcccc attttaagag cgacagatcg gcttgaacgg cgattggccg
     6541 gagaaaaccg agcggtttgc ccggcggcgt cagcaaatac caagctacgg cgacgctatg
     6601 gcaccccatg tcggtcatca cgccaccacc ctgttgaaca gggtcccaga accacgcact
     6661 gtgcggtccc gcgtgttcct ccgacgccct gactagtgtc agagggccca tggtctttac
     6721 cacgggttca agttgcgccc gggcctgctg gaccgccttc atgtggagtt ggttttcaaa
     6781 atagatgaga ggcactccaa acgggcgcaa tcgttcaatc atttgccggg cttcccgaag
     6841 attcctggca agtggcttat cacagatcac ggcccgcagc ttagccccct cgtcgagggc
     6901 tttcccaatc tcttccacaa ttggtacacg tgcgaagttg ggggcgtaaa tggccaccac
     6961 atccacgtga tgggccatct ctcggacgga ggggaaaatt cggccctccc ccaacctttt
     7021 cgcttgaaca aattgcgaca gggcttcagg cgggcggcgc gagactagac cggctacctc
     7081 aatccctcga acttgttcca gcgcacgcag ttgaaacttc gcgtaaaacc caccacccac
     7141 tatgccgtaa cggattgtct ccatctcaca cctccgttgc tggattctcg agtaccgttc
     7201 gcgcgacctg aaccgatgtc gaaatcagaa tccgcctcac cggcgattat gtgctgtcat
     7261 catcgtacgg tctcttagaa ccctccgcaa cccaccgtgt ggtaagtcaa agggaatcta
     7321 acgcgggcca cgtgttgcgc cagtggcact ccaaatttct ttcgttgcga aaaacacgcg
     7381 gggcctcgag atgccgtaat gcttggcgca tgtctcgttt gtcctcctcg ggacagtcaa
     7441 attgccaagc gatccattcg tcctcggcca ggctgtacgg tgtcagggga tcgcgtagaa
     7501 cagcgaagcg acttgccgga actgatagta gagccgacgg agagtgtcgt aatccaactc
     7561 ttttatgcgg atatcaatgt cccacaggga ctgtttagcg aaagcaacca ctgggaacgg
     7621 ttttcaaacg taaccaatgt tacgctgagg ccgcttgctt gatcttgcca aacctgcaca
     7681 tatcgggtgc gattgagg
//
LOCUS       DAOPTR010000029         7648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_7950_length_7648_cov_3.503622, whole genome shotgun sequence.
ACCESSION   DAOPTR010000029 DAOPTR010000000
VERSION     DAOPTR010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7648
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7950_length_7648_cov_3.503622"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PKI05_01115"
     CDS             <1..496
                     /locus_tag="PKI05_01115"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOA50823.1"
                     /translation="LQNKNHEVIFHDLYQERFDPLLPADQIPKDATVPPEIEEHCRQL
                     READGYVIVHPNWWAQPPAILKGWIDRVFRQGVAYDFSAEGVVGHLKGKRALVFTTSN
                     TPREDELRLYGDPLENLWKNCIFGFCGLTHFRRRNFESIVMSTYEQRVGWLEEVRQIV
                     ESFF"
     gene            867..2210
                     /locus_tag="PKI05_01120"
     CDS             867..2210
                     /locus_tag="PKI05_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOA50824.1"
                     /translation="MNHFLQVVQIALRRPLWIVAIAVTALFVGVMWGGNITAIYPIVE
                     VAFRGESLHYWIDLKIQECDEEIARLQEEIAAAGGTEADGPSADGSDREAGTSHRPVR
                     SGQLSQLLVKRDWYLWLRPWILRLTPNDAFSTLLFILSLLLIGTVLKSVALVINSVLV
                     ARVAHQTVFELRRRFFAHVLRLELSKPGDEGVAHLMSRFTHDINCIYSGLSVLLGKMV
                     REPLKMLACLVGAAWVCFPLLVFSLLVVPPAAWAIWKLGKLLKRANRRAMEEMAQLYG
                     TLEEALRSLRVVKAYTMEPYERRRFHETSRRYLNKGMRIAVFDALTHPLTEILGMSII
                     SLAMAAGAYLVLNEKTHLLGLTVSSRALTMPELVLFYALLVGAADPARRLSDIFASLQ
                     SAMAASDRVFAMLEREPEIKDRPLARRIPRHRRDLVFDHVWFSYTPDQPVLRDIN"
     gene            2214..2876
                     /locus_tag="PKI05_01125"
     CDS             2214..2876
                     /locus_tag="PKI05_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOA50825.1"
                     /translation="MSFGETIVIVGASGSGKSTLLSLIPRFFDPNRGEIRLDGVPLPD
                     IRLRSLRKQIGLVPQEPVLFNDTILNNIKYGSPQATDEQAIEAARAAHAHTFIEQLPQ
                     GYNTLLGPMGCGLSGGQRQRIALARAMLRDPAILILDEATSQIDVESEQLIHQALERF
                     AQGRTTIIVTHRLNTIAMADRILLLSGGQIVGLGKHHELLEQCELYRRLFAGSMWELR
                     AA"
     gene            3078..3776
                     /locus_tag="PKI05_01130"
     CDS             3078..3776
                     /locus_tag="PKI05_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011120140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA50826.1"
                     /translation="MVFPQDESPLLVLRDVRKSFQEPDGGELPILDIPEFTVEAGEQV
                     VIVGESGCGKTTLLHIIAGISRPDEGQVRINGWDITLLSEAECDRFRAENIGYVFQTF
                     NLLPAFTALENVLLGMTFGRGKPDVARAKHLLERVGLAHRMHHKPRQLSVGEQQRVSV
                     ARALANRPKLILADEPTANVDSGNQQAVLDLLREGCREENVALILVTHSPEVARQFPR
                     IVRLPDINLVLTRK"
     gene            3864..5177
                     /locus_tag="PKI05_01135"
     CDS             3864..5177
                     /locus_tag="PKI05_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA50827.1"
                     /translation="MPLWKIAWRSIQHRAVASALTAFSMALGVALVVAVLVIYHVLDR
                     SFRRSSQGYDMIVGPAKGSPLEIVLSTVYYLQAPRGTIPYEIYEELSHGRFATEVAAA
                     IPVCIGGSYAGFPVVGTVPELFEEFRYLGDRPYEFEPGGRNFSPDAPFEAVIGATVAR
                     RAGLRVGDTFRVTHGLAEQGGHEHPDEFTIVGVLKPTGTPNDRALFVNIEGFWKLHQH
                     DARHGKASEEEESVNPMDGDGDEATSETEAHDENHRELTAVLVKRDWTNSAKALALPM
                     QVSKEPGVQSVIPTEEVAKLFEGLLGNIQTILLIFAVLIVVVAGIGLMVSIYNSMNER
                     KLEIAIMRALGAKRSTVMAVILLESILLSLGGGAIGVLLGHGLIGAFSGVILEETGVR
                     VSALSFQTVELILVPGLIILASVVGYLPALVAYRTDVAKAIASGV"
     gene            5335..6102
                     /locus_tag="PKI05_01140"
     CDS             5335..6102
                     /locus_tag="PKI05_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50828.1"
                     /translation="MRKYVLSLVTVVGMAFALVAGTAMAQPPGGPGGPGGRGPGMFMM
                     GPGGGVGYAQLLRIEKVQKEIDMTDDQKAEVEKILEKSRERMRELFQGGGQDREAARQ
                     RFQQAQQETQKAIEGVLLPDQVKRLKEIRLQVIGIGAAMMDQEVRKELGVTEEQAQKI
                     RDAVQKVMEELRGQRQQGERPSPEQMRERMQQMQQKMEEAVMSQLTEEQKNKWKSMIG
                     EKFELDRSELFGGFRGGPGGAPGGAPGGQRRGGNRGA"
     gene            6647..>7648
                     /locus_tag="PKI05_01145"
     CDS             6647..>7648
                     /locus_tag="PKI05_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOA50829.1"
                     /translation="MEAGEILVRKGLLTPQQLQLARRHQTEGKRLDQAAVGMGLVSEE
                     DALRALGEELGIEYIDLSEATIDPSILKNFPVKFIYRESLFPIREENGRLLVATSDPF
                     NLYPLDELATATGKTVVPVLASQAEIAKLIKTYLGVGGETIEELMAQGEEEESAVELL
                     DEIETDGSELSKLAQEPSVVRLVNEILLEAIDLRASDVHLESQPSGLKIRYRIDGVLH
                     PQPVPPEINRFHAAIISRLKIMARLNIAERRLPQDGRIQLRVAGREVDVRVSVIPMIH
                     GEGIVLRLLDKGAMEFDLRKLGMEQDVYEKFRELIQLPHGILLVTGPTGSGKTTTLYS
                     "
BASE COUNT         1575 a         1985 c         2325 g         1763 t
ORIGIN      
        1 tctgcaaaac aagaatcacg aggtcatctt ccacgatttg tatcaggaac ggttcgatcc
       61 tctgttgccc gcggatcaga ttcccaaaga tgcaacggtt ccaccggaga tcgaggaaca
      121 ctgccgccag cttcgagagg ccgacggata cgtgattgtc catcctaatt ggtgggcaca
      181 accgcctgcg atcctcaagg ggtggatcga ccgggtcttt cgccagggcg tggcgtatga
      241 tttctcggca gagggcgttg ttgggcattt gaaggggaag cgggcactcg tctttacgac
      301 ctccaacaca ccccgcgaag acgaactccg gctttacggc gaccccttgg agaatttgtg
      361 gaagaactgc atttttggtt tctgtggtct cacccatttc cggcgacgta atttcgagtc
      421 gatcgtcatg agcacctacg agcagcgcgt cggctggttg gaagaggttc gtcagattgt
      481 ggaaagcttt ttctaaaccg ggaccccgtc ggtcgccatg agacgcccgg acgcgacaga
      541 gcgcgttcgt cgcaaacggt aggagttttt taaaggacgc gggggacccc gtccctccca
      601 gatggcgtcc ctccagaatg gccgacgggg gtttatctgg tctcggattg atctttgtaa
      661 agctgcaaag atttggtaaa aaaggcgtta gcccgaacga aagccgtgtg ttttttcgcg
      721 cgggttagag cctatcccat aatctcgcct cgcccggtcg gcgggagagg gggctttttt
      781 gggatagact ctcgattgct tgcctgcttg cctggcatct ttcggacgag cttggttttt
      841 gaagacgacg gagtcagtgt tcaaggatga accattttct tcaagttgtc caaatcgctc
      901 tccgccgccc cttatggatt gtcgcgattg ccgtcacggc cctgtttgtt ggcgtcatgt
      961 ggggaggaaa catcacagcg atctacccga ttgtagaggt ggcctttcgc ggagagtcac
     1021 tccactattg gattgatttg aagattcagg aatgcgacga ggagatcgcc cggctgcaag
     1081 aggagatcgc cgccgcgggg ggcacggaag cggacggacc ctcggccgat gggtcggacc
     1141 gtgaggccgg cacatcgcat cgaccggtcc gatcggggca actttcgcaa ctgctggtta
     1201 agcgagattg gtatctgtgg ctgcggccgt ggattcttcg cctcacgcca aacgatgcgt
     1261 tttccacgct ccttttcatt ttgagcttgc tgctaattgg gaccgtgctg aaaagtgtgg
     1321 cgctggtgat caattcggtc cttgttgccc gcgtcgccca ccaaacggtc tttgagcttc
     1381 gccgtcggtt cttcgcgcat gttctgcggc tggaattgag taagccgggt gatgaaggtg
     1441 tggcccacct catgagtcgc ttcacacacg acatcaattg catttattca ggtctttccg
     1501 ttctcttagg gaagatggtc cgcgaaccgc ttaagatgtt ggcctgttta gtgggggcgg
     1561 cctgggtctg ttttccgctg ctggtttttt cactcttggt cgttccgcct gccgcctggg
     1621 cgatctggaa attggggaag ctcctgaaac gggccaaccg ccgggccatg gaagaaatgg
     1681 cccaacttta cggcacattg gaagaggcct taagatcgct gcgcgtggtg aaggcctaca
     1741 ccatggaacc gtacgaaagg cggcgctttc atgagacgag ccgtcgctat ctgaacaagg
     1801 gcatgcggat cgccgttttt gatgccctca cgcatcccct aacagaaatc ttaggaatgt
     1861 cgattatcag ccttgccatg gcggccggtg cgtatctcgt tctgaacgag aagacgcatc
     1921 tgcttggctt gaccgtctcc agccgtgcct tgacgatgcc ggaattggtt ctgttttatg
     1981 cgctgcttgt tggcgcggcg gacccggcga gacgactttc cgatattttc gctagccttc
     2041 agtcggccat ggcggcttct gaccgtgtgt ttgccatgct ggagcgcgaa cccgagatca
     2101 aggaccgccc tttggctcgg cgaattcctc gtcaccggcg ggatttggtc ttcgatcacg
     2161 tgtggttctc ctacacgccc gatcagcccg ttctccgcga cataaattag gaaatctcgt
     2221 ttggtgaaac gatcgtcatt gtgggagcaa gtggcagcgg gaagagtacc ttgctcagtc
     2281 tcattccccg gttctttgac cccaatcgag gggaaattcg cttagatggt gtgccccttc
     2341 ccgatattcg cttgcgctct ttgcgaaaac agattggcct ggtcccgcag gagccggtcc
     2401 tcttcaacga cacgatcctg aacaacatca aatacggttc gccacaagcg acggacgagc
     2461 aggcgatcga ggctgcccgc gccgcccatg cccacacgtt tatcgagcag cttccccaag
     2521 ggtacaacac gcttctcggg ccaatggggt gcggactctc tggagggcag cgccagcgga
     2581 tcgccctggc cagggccatg ttaagggatc cggccatcct tatccttgat gaagccacca
     2641 gtcaaattga tgtggaaagt gaacaactca tccatcaggc cctggaacgc ttcgcccaag
     2701 gacggaccac catcatcgtc acgcaccggc taaataccat cgccatggca gaccggatcc
     2761 ttcttctcag cggcggccaa attgtgggct tgggaaaaca tcatgagctt cttgaacagt
     2821 gcgaacttta ccgccgcttg tttgccggtt cgatgtggga actgcgtgca gcttaacagt
     2881 taccagcgcc cggccttgtg accctcatca cggatacctt tacccgcgcc aaataacccc
     2941 gctggacggc gtgggcgtgt acaatgagcg aactggggtc tatccgcgga cgtagcattt
     3001 gcaatcagat cgaactcaag ccctgggaaa atacgttgta tttgtgtctg tttcttttga
     3061 cataagtctg gtcggccatg gtattcccac aagacgaatc accgctcttg gtgctgcgtg
     3121 atgttcgcaa gtcgtttcaa gagccggacg gcggtgaact acccatcctt gatatcccgg
     3181 agttcacggt agaggcgggc gagcaggtgg tgattgtcgg agaaagtggc tgcggaaaga
     3241 ccacgctcct ccacattatt gcggggatta gccgaccgga tgaggggcag gttcgcatca
     3301 atgggtggga tattaccctc ctatcggagg ctgagtgcga tcgtttccgg gcggaaaaca
     3361 tcggctacgt gtttcaaacg tttaaccttc ttccggcctt cacggccttg gagaatgtcc
     3421 tcctggggat gacgtttggt cggggaaaac ccgacgttgc ccgcgccaaa caccttttgg
     3481 aacgcgtggg gttggcgcat cgcatgcatc acaagccacg gcaactatcc gtgggggaac
     3541 agcagcgggt atctgtcgcc cgtgccctag ccaaccggcc aaaactgatc ttagccgatg
     3601 aaccgacggc caacgttgat tcgggtaatc agcaggcggt gttagacctt cttcgggagg
     3661 ggtgccggga agagaacgtg gcgttgatcc tggtgacgca tagcccggag gttgcccgac
     3721 agtttccccg catcgttcgc ctccccgaca tcaacttggt cctgacacgg aagtgacgtt
     3781 tggaaatgat gttacgccta acagtcgaaa acggtcaatt agcttagttg tgccatcaaa
     3841 gaacgtcata gggtcctgat aagatgcctc tttggaagat agcctggcgg agtattcaac
     3901 atcgcgcggt ggcctcggct ttgacggcct tttccatggc cctgggggtg gccctggtgg
     3961 ttgctgtgct ggtcatctat cacgtgttag accggtcttt ccggcggagt tcccaggggt
     4021 acgatatgat cgtcggtccc gcgaaaggca gtcctctaga gattgtcctg agcaccgtct
     4081 attacttgca ggcgccgcgg ggaaccattc cttatgagat ttatgaagaa ctttcacacg
     4141 gacgctttgc taccgaggtg gcggctgcca tccccgtttg tattggcgga agttatgccg
     4201 gcttccctgt ggtggggacc gtcccggagc tttttgagga gttccgttat ctgggggatc
     4261 ggccttacga gtttgagccg ggcggacgga atttctcgcc cgacgcacct ttcgaagcag
     4321 tgatcggcgc gaccgtggct aggagggcgg gcctgcgggt gggggacacg ttccgcgtga
     4381 cccacggcct ggccgaacaa ggaggccacg agcatcccga tgaatttacg attgtggggg
     4441 ttttaaagcc cacggggacc ccgaacgatc gggctttgtt tgtcaatatc gaaggctttt
     4501 ggaaactcca tcagcatgac gctaggcatg gtaaagcctc cgaggaggaa gaaagcgtta
     4561 acccgatgga cggagatgga gacgaggcga cgagcgagac cgaggctcat gacgagaatc
     4621 atcgcgaatt gacggccgtc ctagttaagc gggactggac gaactcggcc aaggcactgg
     4681 ctttgcccat gcaggtgagc aaggagcctg gggttcaaag cgtcatcccc accgaagagg
     4741 tggccaagct ctttgaggga ttgctgggaa acatccagac gattttgcta atttttgcag
     4801 ttcttattgt ggtcgtagca ggcattggcc taatggtgag catttacaac tcgatgaacg
     4861 aacgcaaact ggagatcgcc atcatgcggg cactaggggc gaagaggagc accgtaatgg
     4921 cggttatcct ccttgagtcg atacttctat cgctaggagg tggggccatt ggcgttctgt
     4981 tgggacatgg cctaataggg gcgttctcgg gggtcatttt agaagagacc ggtgtgcggg
     5041 ttagtgccct cagctttcaa acggtggagc taatcctggt gcccgggttg attattctcg
     5101 cctccgtggt gggttacctg ccggcgttgg tagcctatcg caccgacgtt gccaaggcca
     5161 tcgccagcgg ggtctgaaaa aaggtttatg gttccgccgc ggaactttct gcccccaccg
     5221 ctgttaaatc tcttggaggg tggtggacac cttctgaccg gttcgctttt catgaatacc
     5281 ggtctttcgg atcgtgtttg aggtgtttgg tgtttcctgt tcggaggtcg tctcatgagg
     5341 aagtatgtct tgagccttgt gacagtggtg ggcatggcat tcgcgttggt ggcgggcacg
     5401 gcgatggccc aaccaccggg agggcctggt gggcccggtg gccgcggccc cggcatgttc
     5461 atgatgggcc cgggtggcgg cgtgggttac gcgcagctcc ttcgcattga aaaggtccaa
     5521 aaagagatcg acatgacgga tgatcagaag gctgaggtag agaaaatcct tgagaagtcg
     5581 cgggaaagga tgcgggaatt gttccaggga ggcggacaag atcgagaggc tgctcggcag
     5641 cggttccaac aggcccagca ggaaacccag aaagccattg aaggcgttct gcttcccgat
     5701 caagtcaagc gactcaagga gattcgtctg caggtcatcg gaatcggcgc ggccatgatg
     5761 gaccaggaag tccggaaaga attgggcgtg accgaggagc aggcccaaaa gatccgtgac
     5821 gccgtccaga aggtcatgga agagttgcgc ggccagcggc aacagggtga gcgtccaagc
     5881 cctgagcaga tgcgagagcg gatgcagcag atgcagcaga agatggaaga ggccgtcatg
     5941 agccagttga cggaagaaca aaagaacaaa tggaagagca tgatcggcga aaagtttgaa
     6001 ctggatcgct ccgaattgtt cggtggcttc cgcggcggtc ctggcggggc accgggcggc
     6061 gctcctggtg gtcagcgccg tggcggaaac cggggcgcct gagattgccc ccgcgggcct
     6121 gtcccgctgc gtcggctcca tcacaaggaa agcccccgat tcccgcaaat tatgctagaa
     6181 cattacgctg ttcgcgggca ataaatcccc acggaatcgg gggctccttt aatgatgttg
     6241 ggagtatttg cagtccagcg tctttatttg tagccttttg tcaacgaaat gtgttaacta
     6301 tttggtcacg ccttgattgc tcttataatg tgttgacaga gttttgggac gtgcggttca
     6361 gcgctctcat gcaatggaat gagtgtgggg tatcggctga actctcccgc gttaagtgtt
     6421 tgcatctttc cgatcgggcc atccagaaaa ataacctccc tgataacgat aggtttgttt
     6481 cgccaagcca aggttttgat ttgagcggtg cgagacagga gatcagtagc ttttgatcca
     6541 ggtgactgtt ttttaggttt ggccttcgtg ggcagcgttt gtcggtggcg taattcgggc
     6601 aaaatttgac ggttggcgtc accaaaatgg gaatgatagc gtgattatgg aagccggaga
     6661 aattttggtt cgcaaaggtc ttcttacccc gcagcagctc cagttggccc gacgccatca
     6721 gactgagggt aagcgactgg atcaggctgc ggtggggatg gggctggtct ccgaggagga
     6781 cgcccttcgc gcgcttggtg aggaattggg gatcgagtac attgatcttt ccgaggccac
     6841 cattgatccc agcatcctca agaatttccc tgtgaaattc atctaccggg agtcgctctt
     6901 tcctatccgg gaagagaatg gccgactgct cgttgcgacc agtgatccgt tcaatcttta
     6961 ccccctcgac gaattggcca ccgccacagg gaaaacggtc gtccctgtcc tggccagcca
     7021 ggccgagatt gccaagctca tcaagaccta cttgggtgtg ggcggtgaga ccatcgagga
     7081 gttgatggct cagggggagg aagaagaaag cgctgttgag ctcctcgacg agatcgaaac
     7141 tgatggttcc gagctatcga agttggccca ggaaccctcg gtggttcgat tggtcaatga
     7201 aatattgtta gaggcgatcg atctgcgagc gagtgacgtg caccttgaat cgcagccgtc
     7261 tgggctcaag attcgttacc ggattgacgg cgttttgcat cctcagccag tcccgcccga
     7321 gatcaaccgg ttccacgccg ctatcatcag ccgcctcaag atcatggcaa ggctgaatat
     7381 tgccgagcgg cggttgccgc aggacgggcg catccaactc cgcgtggccg gacgcgaggt
     7441 tgacgttcgt gtctccgtca tccccatgat ccacggcgaa gggatcgtcc ttcgtttgct
     7501 ggataagggc gcgatggagt tcgacctccg gaaactgggg atggagcaag atgtttacga
     7561 gaaattccgt gagctgatcc aactgccaca cggcattttg ctcgtgacag ggccgaccgg
     7621 gtcgggtaag accacgaccc tttacagt
//
LOCUS       DAOPTR010000030         7638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_7965_length_7638_cov_3.209943, whole genome shotgun sequence.
ACCESSION   DAOPTR010000030 DAOPTR010000000
VERSION     DAOPTR010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7638
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7965_length_7638_cov_3.209943"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="PKI05_01150"
     CDS             <1..756
                     /locus_tag="PKI05_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOA50830.1"
                     /translation="AFFFASPSHQASLLKLRYAIEQREEAALLVGEAGTGKSFLTHVL
                     TESTNESVAPVVRTCCLFDAPEELMTHLAAEFVGRNFNGSGYAALQELEAFLGRNLEE
                     GHHAVLILDDSHLLQSIRSWEVLRSLMTLKIQGETPWTLIVLAQPLFLPLLDRLPQLE
                     ELFALKAFLRAWTPEETSQYIKHCLTTAGCDEDVFSPDAVEVVARFSSGIPRRINRLC
                     DLSLLVAFAEKRYQVTAAHVEAVHEELMTVPCQ"
     gene            829..2646
                     /locus_tag="PKI05_01155"
     CDS             829..2646
                     /locus_tag="PKI05_01155"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HOA50831.1"
                     /translation="MRSHLSRGLRLQVGRPQRAIWCGVCAALVAAGALLGTSPWTPMT
                     WDEGDAIRRAESIRDWFSHAFRMEASGPPALSREGITRGWPFTVRREGHPALYAAIIA
                     LGRSLSPGFLDPLTRYRFGPIVLFAAAMGAVTYRVARIAGVSPAVAAFLCILLQPRLF
                     AHAHFASFDGPLTAWWMLAWACSPFFSTMRTSHKRTSRSDLARIFLWGLILGATMSSK
                     ATGWLVVVPFLFWAIGQRQWSVLFRTIVGILIGLGTFVVLNPPLWYDPISGVLRFFQL
                     NLSRHERPDLNITTFFLGELYNLDYPLPWYNTLFWTAIVVPIPILALFIVGLVYWVRT
                     SRRSILTLVVLHWLTLIVVRAIPGTPPHDGIRLFLPAFPFLGILAAFGFRFVTSAPII
                     LLRRWAMGFLPLPRRQRSIRAAQPLAETRSFSWRRNFLHTAHSVLQTVMWLVVLTPLI
                     NLICFGPQWLSYYNGLIGGLPGATAAGMEPTYYWDGLDREVFLWLEIHCLETEKVYYP
                     ACPWTNLRLLQEWGVLKRAFATSPAEAQWYVVQNRPGAWFPEDKWLFTHGEAAFTKYP
                     GRGPMCPWVGRVPVVKIFPIREYFRACQAVHANRAMSDQ"
     gene            complement(2750..3856)
                     /locus_tag="PKI05_01160"
     CDS             complement(2750..3856)
                     /locus_tag="PKI05_01160"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOA50832.1"
                     /translation="MAKTVRVLIVDDSIVMRQMIKDFLVTAPGIQVVGMASNGREALR
                     LADELAPDVITLDIEMPGMDGLSTLDTLLAKRPIAVIMVSSLTQAGAAITLDALERGA
                     IDYLTKPAHKSEAATVFGQELIRKIRFAATSDVNRIVRLRKQRAQSLRMAEPVVKSPT
                     ESLEGPPTLSRACIAIGISTGGPPALTSLFRELKPPLPPILVVQHMPPQFTKPFAWRL
                     NSVSRLTVKEAESGDLVKPNHAYVAPGGFHMAVQGRSGSARIRIFDGEPVSGHKPSVD
                     VMMSSVAQVFGRRVLGVIMTGMGRDGANGCRAIRAAGGYVLGQDEATSDVYGMNKVAF
                     VEGNVDRQFPLQEGARLIMRYVEEKFLAQPSEVL"
     gene            complement(4137..5405)
                     /gene="hemA"
                     /locus_tag="PKI05_01165"
     CDS             complement(4137..5405)
                     /gene="hemA"
                     /locus_tag="PKI05_01165"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HOA50833.1"
                     /translation="MEICVVGCSHQTAPLAIREKLAFSPEQTRAALQLWEKRFPWVEA
                     VLLSTCNRVEIYLASEMATIPPATDVVDFLGECHGLTRQDFADLCFALCTPQSLEHLF
                     RVAAGLESMVLGEPQILFQVKQAYQWATEMGTVGPLLHIVFQGAFRAARRVASETEIQ
                     KRRVSIPSIAVSDFGRAVFERFEDKLVLVIGAGKMAEETIRYLRSEGAQQIVIINRHL
                     ERAHQLAERCQGEVRPWEELAAVLREADVVVSTTGAEQPIVTREMLSRVIAARLERPL
                     FVLDLAVPRDFEPAIATMPGVYLYCLDDLMDVCQKNREARDKELPRADEIVAQEVGSV
                     LANLRQRTVGPVIQQLRKDWESVKEQELRRLFNKLSDLDPAIQAEIRLAFDRLINKLL
                     HPPLSSLREYSQSGAHQEMIEAIRKLFRHS"
     gene            complement(5518..6558)
                     /locus_tag="PKI05_01170"
     CDS             complement(5518..6558)
                     /locus_tag="PKI05_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50834.1"
                     /translation="MGEMIAIMVCAYGLAFLMELGRARGWHVSTRLIGGLLVLGLIVH
                     TGWLIYRIALFQEQVELGGHVLSIRQDWYYGIAWGLALICLFWMIIRPATPFGVFFLP
                     LILLLIVLGVVWGDTVPFARGAASRSWGIIHGFSILTAILAVCVALLSGVMYLGQVYR
                     LKRRAIATSRFRLPSLEWLHNVTKNALFVAVAMLAVGIFSGLWLNVVQYSTLQGHLPW
                     NDPVVLSTWILFFWLGGSLLLGFVYRPLREGRRVVYLTIVSFVFMLLVLGMTLFGATR
                     HGQQRELPDGGLPVKSTQWQGMDPWKYLGKKRAFCLKSSAVSLMPEPGMSRKSGFLNC
                     KKTQGYSATGTQ"
     gene            complement(6661..>7638)
                     /locus_tag="PKI05_01175"
     CDS             complement(6661..>7638)
                     /locus_tag="PKI05_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008694157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOA50835.1"
                     /translation="ETGTGKELIAKAIHQLSPRRSGPFVRVNCGALPEGLLESELFGH
                     VRGAFTGAIENRTGRFEAAHTGTIFLDEIDSTTFKLQVKLLRVLQQHEFERVGDTRTI
                     HVDVRVIAASNRDLLEEVEKGRFREDLYYRLNVVTIYLPPLRERREDIPELVGHFLRL
                     YNEKNRRYVPFVHPKAMEALKNYDWPGNVRELQNYVERAVILAPGDEVTCDLLPDAVL
                     GRRMPRMGYRRKDLDELARDLVAAALAQFPPDHKQLYQEIVGRVEKELIHQVLARCNQ
                     VQIKAAAQLGLNRNTLRKKLKEWGWVDATEAEDDGSASRDLPLPSEETF"
BASE COUNT         1641 a         2265 c         2017 g         1715 t
ORIGIN      
        1 gcattctttt ttgcgagccc ctcccatcag gcctctctgc tgaagcttcg ctacgccatt
       61 gagcagcgcg aagaagcggc cctcttggtt ggcgaagctg gcaccggtaa gagttttctc
      121 acccacgtgc tcaccgaatc gacgaacgaa tcggtcgctc cggtcgttcg tacgtgctgc
      181 ctttttgatg ccccagaaga actgatgaca cacttggcgg cagagttcgt tggacggaat
      241 ttcaacggct caggatatgc cgctcttcag gaactcgaag ccttcctcgg acgcaatctt
      301 gaggaaggcc accatgccgt gctgatcctc gatgactcgc atctcttgca aagtatccga
      361 tcgtgggaag tcctgcgctc gctgatgacg ctgaagatcc agggcgaaac accctggacc
      421 ttgattgtcc tggcacagcc cctcttcctg ccattgctcg atcgactgcc tcaattggaa
      481 gaattgtttg ccttaaaagc ctttttgcgg gcatggacac cggaggaaac atcccaatac
      541 atcaagcact gcctgaccac ggccggatgc gatgaggatg tcttttctcc cgacgctgtg
      601 gaggtcgtag cacggttttc ctccgggata ccgcgtcgca tcaatcgctt gtgtgacttg
      661 tctctcctcg tggcgttcgc cgaaaaacgc taccaagtga cggctgccca cgtggaggcc
      721 gtccacgaag aactgatgac cgtgccctgc cagtgagagc gatcttcacc gccatcagtg
      781 cttcaggccc atggtataat cgagagcatg ggcaacgatc tgggtttcat gcgttcgcat
      841 ctgagtcgcg gacttcgtct tcaagttggt cgtcctcaaa gggccatttg gtgtggtgtt
      901 tgtgcagccc tcgttgccgc aggggctctg ctcggcacct cgccgtggac gccgatgact
      961 tgggacgaag gtgatgcaat ccgccgcgcg gagagcattc gcgattggtt cagccatgct
     1021 ttccgcatgg aagccagtgg acctcctgcc ctctcgcggg aaggcattac gcgcggttgg
     1081 ccatttaccg tccgtcggga gggccatccc gccttgtatg cggcaatcat cgccctggga
     1141 agaagcttat cgcccgggtt tctcgatccc ctcaccaggt atcggtttgg ccccattgtc
     1201 ctattcgccg cggccatggg ggccgtaaca tatcgcgtcg cccgtatagc cggcgtaagc
     1261 cctgctgtgg cggcatttct gtgcatcctt cttcaaccga ggctctttgc tcacgcccat
     1321 ttcgcctcgt ttgatggccc cctgacagcc tggtggatgt tggcctgggc gtgctctcct
     1381 ttcttttcaa ccatgcgaac atcacacaaa agaacgtccc gaagcgatct cgcccgcatc
     1441 ttcctttggg gcctcatcct cggggcaacg atgagtagca aggccacagg gtggctcgtg
     1501 gttgtgccct ttctcttttg ggcgataggg caacgccagt ggagtgtcct ttttcggacg
     1561 atcgtcggta tccttatcgg actgggaacc ttcgtcgtgt tgaatccgcc gctctggtac
     1621 gatccaatct cgggcgtttt acgcttcttc caacttaacc tgagtcggca cgagcgcccc
     1681 gatctgaata tcacaacgtt cttcctgggc gagctttaca accttgatta tcccctgcca
     1741 tggtacaaca cgttgttctg gacagcgatc gtggtgccca ttcccatcct ggcgctcttc
     1801 atcgttgggc tggtctattg ggtccgcaca tctcggcgca gtatcctcac gcttgttgtc
     1861 ttgcattggc tgacacttat tgtcgtccgc gcgatccccg ggacaccccc gcatgatggc
     1921 atccgtttgt tcttgcccgc ttttcctttt ctgggcatct tggcagcttt tgggtttcgc
     1981 ttcgtcacat ccgcccccat cattcttctt cgacgatggg ccatgggatt tttgccactc
     2041 ccacgaaggc aaagatcaat ccgagctgcg caaccgttgg cggaaactag atccttctcg
     2101 tggcgcagga atttcctcca tacggcacac tccgttttac aaacggtcat gtggttggtg
     2161 gttttgacac cgcttattaa cttgatctgt tttgggcccc agtggctttc gtactacaac
     2221 ggcctcattg gtgggctacc cggtgccact gccgccggta tggaaccaac ctattactgg
     2281 gatggtctgg atcgcgaagt ctttctgtgg cttgaaattc actgcttgga gactgaaaag
     2341 gtctattatc cggcatgccc gtggacaaac cttcgtttgc ttcaggagtg gggcgtgctt
     2401 aaacgggcat ttgctacctc cccggcggaa gctcaatggt acgtcgtcca gaatcgtccc
     2461 ggggcatggt ttccggaaga caagtggcta tttacccacg gcgaagccgc atttaccaag
     2521 taccctggcc gtggtccgat gtgcccttgg gtgggacggg ttcccgtcgt caagatcttc
     2581 cccattcgag agtattttcg cgcttgccag gctgtccacg caaaccgggc gatgtctgat
     2641 caataaaagc ttacaccgaa atcgataccc cagcggtggc aggcccatct tgcgggaacg
     2701 tcattcgccg gcgtatgagc gtttgaatca tcatcaaatc ggcccattgt caaaggacct
     2761 cacttggttg ggcgaggaac ttctcttcca cgtagcgcat gatgagccgt gccccttctt
     2821 ggagtggaaa ctggcgatcg acgttaccct ccacaaaggc aaccttgttc atcccgtaaa
     2881 catccgaggt cgcttcatct tggcccagca cgtatcctcc tgctgcccga atggcccgac
     2941 atccgttagc tccgtcccgg cccattcccg tcataatgac accaagaacc cgccgcccaa
     3001 agacttgggc aacgctcgac atcatgacat caacagaggg cttatggccg cttacgggtt
     3061 cgccgtcaaa aatacgaatc ctagccgagc cggagcggcc ctgcaccgcc atatgaaatc
     3121 ccccgggggc aacgtaggca tggttgggct tgactaagtc tcccgattcg gcctccttga
     3181 cagtcaagcg gctgacggaa ttgagacgcc aagcaaaagg ttttgtgaac tggggcggca
     3241 tatgctgaac gacaagaatc gggggtaatg gtggtttaag ttcgcgaaat aggctggtca
     3301 acgcgggggg gccgcccgtc gaaatcccga ttgcaataca cgcccggctt agtgtcggcg
     3361 gtccttcgag cgactccgtg ggagacttga ccacgggctc ggccatgcgc aagctttgcg
     3421 cccgttgttt tctgagacga acaatccggt tgacatcgct cgttgcggca aaacgaatct
     3481 tgcggattaa ctcttgtcca aagacagtcg ccgcttctga cttgtgagct ggcttggtca
     3541 gataatcaat ggcaccgcgc tccagggcat ccaaggtgat tgctgcccca gcctgtgtca
     3601 aagagctaac catgatcacg gcaatcggcc gtttggccaa gagggtgtcc aaagtgctta
     3661 gcccatccat accgggcatt tcaatatcca gggtgatgac gtcgggtgct agttcgtcgg
     3721 ccaagcgcaa tgcctcgcgt ccgttggagg ccatgccaac gacctgaatc cccggtgcgg
     3781 taaccaagaa atccttgatc atctgccgca tgacgatcga gtcatcgacg atcaggacac
     3841 ggacggtttt ggccatgcat gatatccttt cctctctggc gatttactct cgggacattg
     3901 atcgagttgg gtcaaaccgt cggccaatcc gtctctcacg gctaatcgcg gaagaaaccg
     3961 acgtttctcg agaaatatgg catatttttc gcagagaaag gtgtcatctc tggattcgca
     4021 ccgtccgcag ttggtttgat cacaaacctt attgaggcat caggtgagaa aatcaacagg
     4081 tttggaagaa tcactaggga tggcacaatc gtttcggtta actctccgtg accttctcac
     4141 gagtgccgga agagcttacg aatggcctcg atcatctcct gatgtgcgcc gctttgggag
     4201 tactcgcgga gagaagagag tggcggatga aggagcttat tgatgagacg atcgaatgcc
     4261 aaacggattt cggcctgaat tgcgggatcc aggtcggaaa gcttgttaaa cagacgccgc
     4321 aactcttgct ctttgacgga ctcccagtcc ttgcggagct gttgaatcac cgggccaacc
     4381 gtccgttgtc gcaagttggc tagcaccgag ccgacttcct gggccacgat ctcatccgcc
     4441 cgaggcaatt ctttatcccg tgcctcccgg tttttttgac acacatccat gaggtcgtcc
     4501 aagcaataaa gatagacgcc tggcatggtg gcgatcgcgg gttcaaaatc ccgtggcacg
     4561 gccagatcaa ggacaaagag cggacgctcc aaccgtgcgg cgataacccg gctgagcatt
     4621 tcccgcgtga cgatgggctg ctccgcaccc gtggtgctga ccacaacgtc cgcctcgcgg
     4681 aggaccgcag caagctcctc ccaggggcga acttcgccct ggcatcgttc tgctaattgg
     4741 tgggctctct ccaagtgacg attgatgatg acaatttgct gcgcaccctc gctgcgcagg
     4801 tagcgaatcg tttcctctgc catcttgccg gccccgatga ccagcacgag tttgtcctca
     4861 aagcgttcaa aaacggccct gccgaaatcg ctgacggcga tgctgggaat gctgactcgc
     4921 cgtttttgga tttccgtctc cgatgctacc ctccgtgctg cgcggaaggc cccttggaat
     4981 acgatgtgca aaagaggccc caccgtcccc atttccgtgg cccactggta agcctgcttt
     5041 acctggaaga gaatctgggg ctcaccgagg accatgcttt ccagccccgc ggccacgcga
     5101 aataggtgtt ccagcgactg gggagtgcag agggcaaaac aaaggtcggc aaaatcttgg
     5161 cgcgtcagac cgtgacattc gccgaggaag tcaacgacat cggtcgccgg cggaatcgtc
     5221 gccatttcgc tagcaagata gatttcgacc cgattgcaag tggacagcag aacagcttca
     5281 acccagggaa accgtttttc ccaaagctga agcgctgccc gcgtttgctc gggcgaaaac
     5341 gccaatttct cgcgaatggc taggggtgct gtctggtgac tgcacccaac gacacaaatc
     5401 tccatcagtg gcctattccg tctcaagttc gtgtcacatg ttcctccgga cggttgagga
     5461 catcgctatc ctcacagcaa ttctttattc gacgtccatc ccacgaacat aaccctttca
     5521 ttgcgtgcca gttgctgaat acccctgggt ttttttgcaa tttaggaaac cactttttct
     5581 cgacatcccg ggctcgggca ttagcgaaac ggcggaagat ttaagacaga aagctctttt
     5641 ctttccgaga tacttccaag gatccatccc ttgccattgc gtgcttttaa ccggcaggcc
     5701 accgtcgggc aattcccgct gttggccatg ccgcgtagcg ccgaagagcg tcattcccaa
     5761 taccaggagc atgaagacaa aactcacgat ggtaagatag acaactcgtc gcccttcgcg
     5821 gagcggtcga tagacaaagc ccaaaagcag agatccgccc aaccagaaaa agagaatcca
     5881 cgtggagagc accacggggt cattccacgg caaatgccct tgcagcgtgc tatattgcac
     5941 gacgttgagc caaagccccg aaaaaatacc gaccgccagc atggccaccg ccacaaaaag
     6001 ggcatttttg gtcacattgt gaagccactc taagctcggc agacgaaacc gcgaggtcgc
     6061 tatcgcccga cgcttcaaac gatacacctg ccctaagtac atcacgcctg acagcagggc
     6121 tacgcaaacc gccaagatcg ccgtgaggat cgagaagcca tggatgatcc cccaacttcg
     6181 tgatgctgcc ccgcgggcaa aaggaaccgt atctccccac acaacaccga gcacgataag
     6241 cagcaaaata aggggcaaga aaaacactcc aaagggcgtc gctgggcgaa tgatcatcca
     6301 aaacaaacag atcagggcca atccccaggc aatgccgtag taccaatcct gccgtatcga
     6361 gaggacgtgt cctccaagtt ccacctgctc ttgaaagagg gcgattcgat agatgagcca
     6421 gccggtgtgc actatcaggc cgaggaccaa caatccgccg atcaagcgcg tggaaacgtg
     6481 ccagccacga gcccggccca gttccatgag aaacgccaag ccataggcac acaccatgat
     6541 ggcaatcatt tcacccataa cgtacaccca atcactgaga cttatcaacc tccgcaagac
     6601 agtctgccgg atgtgtttgg gacctcggtc acatggagaa tatcacggtt ccgcgaagga
     6661 tcaaaaagtc tcttcggaag ggagagggag gtcgcgggac gccgacccat cgtcttccgc
     6721 ttcagtcgca tccacccaac cccattcttt aagcttcttc cgcaaggtgt tgcggttcaa
     6781 ccctaattga gctgccgcct taatctgaac ctgattacac cgagccaaca cttgatgaat
     6841 caactccttt tcgacccgtc cgacgatctc ctggtacaac tgtttgtggt cgggaggaaa
     6901 ctgtgcgaga gcggcagcaa cgagatcacg cgctagttcg tccaaatctt tccgacggta
     6961 gcccatccgt ggcatacggc gtcccaggac ggcgtccggc aacaggtcac acgtcacttc
     7021 atcgccaggg gccagaatca cggcacgttc cacgtaattc tgcaactcac ggacattccc
     7081 cggccaatcg tagttcttga gggcctccat ggcctttggg tgcacgaagg ggacatagcg
     7141 gcggttcttc tcgttgtaca gtcgcaagaa atgtccgacc agctcgggga tatcttcccg
     7201 gcgttcacgc agcggcggca gatagatggt gaccacgttg agccggtaat acaaatcctc
     7261 gcgaaatcgg cctttctcga cctcttccag caagtcgcgg ttacttgccg cgatcacccg
     7321 cacatccaca tggatagtcc gcgtgtcgcc cacccgctca aactcgtgct gctgaagaac
     7381 ccgcaacagt ttgacttgca acttgaaggt tgtggaatcg atctcgtcga ggaagatcgt
     7441 ccccgtatgc gccgcctcga aacgcccagt ccggttttct atggcgccgg taaacgcacc
     7501 acgcacgtgg ccaaaaagct cactttccaa aagcccctca ggcagggccc cgcaattaac
     7561 gcggacaaac ggaccacttc ggcgcgggct gagctggtgg atcgccttgg cgatcaactc
     7621 cttgcccgtc cctgtctc
//
LOCUS       DAOPTR010000031         7630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_7978_length_7630_cov_3.328845, whole genome shotgun sequence.
ACCESSION   DAOPTR010000031 DAOPTR010000000
VERSION     DAOPTR010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7630
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7978_length_7630_cov_3.328845"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1825)
                     /locus_tag="PKI05_01180"
     CDS             complement(170..1825)
                     /locus_tag="PKI05_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50836.1"
                     /translation="MVNRTDVLTDAVHLRDLRVPSEDGGWLVVPAFSSLIEVARANAM
                     NLAGCPMKIGEITRPQLAVQARAELIQAAVEWTRQYRNVSVSFEQENGGLPAVYVTGH
                     QPELFHPGVWFKNAILHHAAALGQAVAIHLIIDNDTMKSASVRVLGGDCDRPRMINVP
                     IDDGPSGIPYEDRRIHDADVFRNFGKRVKDSLGGMILDPVVDQFWPLVELRARENPRL
                     GHAIAQARHLWEKRWGWNTLEVPQSVVCEQPSFLRLVWHLWVHRERFREIHNHAVQAY
                     RRVHRLRSRSHPFPELLQVDDWCETPFWTWTVDEPVRRKVFVREVNGGLEITDGIAWK
                     DEVPRGASGFGTLLGWWRDQGNKGRRIRSRAVVTTLWARMCLSDLFIHGLGGAKYDQV
                     TDEIIRQFFQCEPPVYCTATATVRLPVSAGRVTIYDVKCLRQLLRELEFHPERFIPYH
                     RLSSLQRETAERLIEEKWKWIRQEVTWENARNRFLAIRTCNRALQEFLVGEHEELERR
                     LQATIWQLEATQVLSSRDWPFCIYSVTQLDQFFDAVAQSCMAR"
     gene            complement(1836..2558)
                     /locus_tag="PKI05_01185"
     CDS             complement(1836..2558)
                     /locus_tag="PKI05_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOA50837.1"
                     /translation="MKTLTALPVYNESAHVKSVLEEVMRYVSDVLVVDDGSTDGTEQV
                     LQGISGIKTVRHPTNRGYGAALRTAFSYAIAEGYEILVTIDCDRQHEPCRIPQFLAAC
                     SDDVDIVSGSRYLRRFPGDSKPPADRQRINQLITQELNRLLGLNLTDAFCGFKAYRVA
                     SLARLELTEDGYAMPIELWVQAAKHKLKIVELPVPLIYLEEKRAFGGALDHAEIRLGV
                     YRAVLERSLLRAGFNIDGSWQL"
     gene            2864..3874
                     /locus_tag="PKI05_01190"
     CDS             2864..3874
                     /locus_tag="PKI05_01190"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011122039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOA50838.1"
                     /translation="MKIAVFSTKPYDREFLELANQSFGHELVFLEPRLTSQTASLADG
                     FPAVCVFVNDELDARVLLHLHRNGTKLIALRCAGYNNVDLQVAEQLGFVVTRVPAYSP
                     QAVAEHTLALILALARRLHKAYNRVREGNFALDGLLGVDLAGKTAGVIGTGKIGRLVA
                     KRLMAFECRILAFDIRPDEQLQAEGVEYVGYEELFRKSDIITLHCPLMPATYHLINEE
                     SLALMKRGIILVNTSRGALVDSQAVIRALKSGRIGALGLDVYEEEADLFFEDLSNQVI
                     QDDVFMRMLTFPNVIVTGHQAFFTRNAMEAIAKKTLENVTAFERGEEPPGLLHYREAF
                     AS"
     gene            complement(3889..>4105)
                     /locus_tag="PKI05_01195"
     CDS             complement(3889..>4105)
                     /locus_tag="PKI05_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50839.1"
                     /translation="NVIVVAYAKPKTLTKAQLLEVAERLQAAKKFQFYLPEIAEMGRP
                     GGDYVRNGPILTDDYAPTDVLRSLPQD"
     assembly_gap    4106..4205
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4206..5051)
                     /locus_tag="PKI05_01200"
     CDS             complement(<4206..5051)
                     /locus_tag="PKI05_01200"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOA50840.1"
                     /translation="MEKWIKLLEVANKGDCSPEMLLYKRAVKQSRLPIIFFVLTGFVI
                     GTLSPCPNGTTLAADTKVLLEKESLYHYVRVIEEDGIRRLQFRRSGLDFEESAINIHD
                     PLDFPLHYYKLMMAAFLHCPEPKSILFVGLGGGTLPRAIHHYFPDATVDAIELDPVVA
                     DAAKQFFGFEENARVKVYVRDARVQMRQFARENRKYDIVFLDAYRGGYIPYHLTTQEF
                     MELTKSLLKDTGIVVSNLQPGFASYHYQRRTIAKVFANEWSYGRYGNVIVVAYAKPKT
                     LTKAQL"
     gene            complement(5144..5764)
                     /locus_tag="PKI05_01205"
     CDS             complement(5144..5764)
                     /locus_tag="PKI05_01205"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOA50841.1"
                     /translation="MERVIRFILFFSAFVCGWVMMGLEILGGRILSPDFGSGVFVWGS
                     VISVFLLSLSIGYYLGGWASARRPSPYVLAVIILLAGISIVPIAFWYAEVSGWFAELG
                     WNERYGALAAASALFLLPSTLMGMVSPYCIRLLTTSVSRAGASAGTLYAISTIGSFLG
                     CLHTAFYLILSTGIRNSLLVGAAVLAALSVVLIIVSAFAWRREDAS"
     gene            complement(6101..6883)
                     /locus_tag="PKI05_01210"
     CDS             complement(6101..6883)
                     /locus_tag="PKI05_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50842.1"
                     /translation="MGLRLAIALGVCTAMMGHFGLACYAADIPTPPKASAYAPAADLV
                     KAVDDYISRIGETLESQDDFAEFQARVVKDAETIAVLALVLGLHDEKNPYQAAAPALI
                     KAARELSAAAAKDYASAKTAFAALEAAKSSSGDASTLKWEKVASLKELMQQVPLVNSR
                     LKRNMRRFERQADAIAVDAAVIAAISQASMANLDETSQPNQAEKWFTFCADSRDAAAA
                     INKAAKAKNQADAEKALAAMTKSCDDCHAVFHPEAIGKTDVE"
     gene            7205..>7630
                     /locus_tag="PKI05_01215"
     CDS             7205..>7630
                     /locus_tag="PKI05_01215"
                     /inference="COORDINATES: protein motif:HMM:NF014766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50843.1"
                     /translation="MLICDVEFQLIKINPASGGIPERLVLVRVQTDEGMDGWGEARLP
                     WRLGELRERRDMILPLVAGKNVLDLAELSRLEQLSPKLRAALEMACRDAAGKILGQPL
                     CYFLGGTYRTRIPTAHYVGDTDDPDLLDRGQRWLDKGIAN"
BASE COUNT         1831 a         2138 c         1874 g         1687 t
ORIGIN      
        1 cggcgacgtg ttagcttgtg acctcccata ggaaactcct taagttagaa gctcgccaaa
       61 tcacacacac gctgaacgcc accccaattg acacccagcc aagcactgaa taaccacggc
      121 agttcagtta gctgaaatgc taatcgcaac agggtaaagc cgtcaagtat cagcgggcca
      181 tacagctttg cgcaacagcg tcaaagaatt gatcaagctg cgtgacagaa tagatacaaa
      241 aaggccagtc gcgagaagaa agcacttgcg tcgcctctag ttgccaaatc gtcgcctgga
      301 gccttcgctc caactcttca tgttctccga ccaagaactc ttggagtgct ctgttacagg
      361 ttcggatggc gagaaaccgg ttcctcgcat tctcccatgt cacttcttgc ctaatccatt
      421 tccatttctc ttcaatcaaa cgttcggcag tttctcgctg taaggatgac aaacggtgat
      481 atgggataaa tcgttctggg tgaaactcca gctcgcgcaa aagttgccga agacatttca
      541 catcgtagat tgtcacccga ccagccgaaa caggaagccg cactgttgct gtagccgtac
      601 agtaaacggg cggttcacac tgaaagaatt gtcgaatgat ttcgtctgtt acttgatcgt
      661 atttggcgcc accgaggccg tggatgaaca aatcacttag gcacatcctc gcccacagcg
      721 tcgtgacaac cgcccgactg cgaatccggc gtcccttatt accctggtcg cgccaccaac
      781 cgagcaatgt accgaaaccg gaagctcctc tcgggacttc gtctttccag gctatgccgt
      841 ccgtgatttc caaaccgcca ttaacctcac ggacaaaaac ttttcgtcgc acaggttcgt
      901 caacggtcca tgtccaaaat ggggtttcgc accagtcatc cacctgcagc aattctggaa
      961 acggatggga gcgcgagcga agacgatgca ctcgtcgata cgcctggacg gcgtgattat
     1021 gaatttctcg aaaccgttct cggtgaaccc aaagatgcca gaccagtcgc aaaaaactgg
     1081 gttgctcgca gacaacactt tgcggtacct ccagcgtgtt ccaaccccag cgtttttccc
     1141 acaggtgacg ggcctgggca attgcgtgtc cgagccgtgg gttctcacgg gcacgaagtt
     1201 caacgagcgg ccagaactga tcaaccacgg gatcgaggat catcccgcca agcgaatctt
     1261 tcacccgctt gccgaaattg cggaaaacat ccgcgtcatg aatccgtcgg tcctcgtaag
     1321 gaattccgga tgggccatca tcgatcggga cgttgatcat tcgcggacgg tcgcagtcac
     1381 cgccaagaac ccggacactc gccgatttca tcgtgtcgtt gtcaatgatc aagtgaatcg
     1441 cgacagcctg ccccagcgcg gcggcatgat gaaggatggc attcttgaac cacacgccgg
     1501 gatgaaataa ctcaggttga tgtcccgtaa cataaaccgc cggaaggccc ccattttcct
     1561 gttcgaagga gacactcacg ttgcggtatt gtcgtgtcca ttcgacggcc gcctggatga
     1621 gttccgcgcg tgcctgaact gccaattgcg gacgggtgat ctcgccgatc ttcatcgggc
     1681 aaccggcgag gttcatcgca ttagcgcgcg cgacttctat caagctggaa aaagccggta
     1741 cgacgagcca tcccccatcc tctgaaggga cccgaaggtc tcgcaagtga acggcatccg
     1801 tcagtacgtc tgtacgattc accatgatgc tttggttaca gttgccatga cccatcaatg
     1861 ttgaaaccgg ctcgcaacag gctgcgttcc aagactgccc ggtacacacc tagtcggatt
     1921 tccgcatgat cgagggcccc accaaaggcc cttttctctt ccaaataaat caaaggcacc
     1981 ggcagttcga caatcttcag tttgtgtttg gccgcctgga cccataattc gatgggcatc
     2041 gcgtacccat cctcggtcaa ttctaaccgc gcaagcgagg ccacccgata cgccttgaac
     2101 ccgcagaagg cgtccgtgag attcagcccc aaaaggcgat tcagctcttg cgtaatgagc
     2161 tggttgattc gttgtcgatc cgcaggcggt ttcgagtcgc ccggaaaccg gcgaagatag
     2221 cgacttccgg aaacgatatc tacatcatca ctacaggcag ccaagaattg cgggatacga
     2281 cacggttcgt gctgacgatc acagtcgatc gtgacgagga tttcgtaacc ctcggcaatg
     2341 gcataggaaa acgcggttct caaagcggca ccgtagcccc ggttggtggg gtgacggacg
     2401 gtcttgatcc cagagatgcc ctgtaagact tgctccgtcc cgtccgtgga accgtcatct
     2461 acgaccagga catccgatac ataccgcatc acttcctcaa ggacactctt aacatgtgcg
     2521 gattcattgt aaacggggag tgccgttaat gtcttcatat tctgcaaaga ccacccaagt
     2581 cccaggaaca accgtggcta tctatcccca tggcttatca cctttgccga caacattctt
     2641 catcatacac gacctgtcca gcgcggctgg catcgacggc aatgccgggc gcatagaatt
     2701 gtagatacca attgcccggg aggtgaaccg atcactgttg gacaacgttc ggaagacgat
     2761 tgccaaccgt ttgcataact gccgagtatt accgcggaac acaaataggt aaactcaccc
     2821 tggtgttagg ctttcgttgg gcacttgttc aagtggacat ttcatgaaaa tcgcagtctt
     2881 tagcacaaaa ccctacgacc gagagtttct ggaattggcc aatcagtctt ttggccacga
     2941 acttgtgttc ttggaacctc gcctcaccag tcaaacggcg tcgttggccg acggattccc
     3001 tgccgtgtgc gtcttcgtca atgacgaact cgacgcgagg gtattgctcc atttgcatcg
     3061 gaacggaacc aaactcattg cccttcggtg tgccggatat aacaatgttg acttacaggt
     3121 agccgagcag ttgggcttcg tggttactcg ggtccccgcc tattctcctc aagccgtggc
     3181 cgaacatacc ctggcgttaa tcctggcctt ggcccggcgt cttcacaagg cgtacaaccg
     3241 cgttcgggag gggaatttcg cccttgatgg tcttctcggg gttgatctgg cgggaaagac
     3301 agcaggagtt atcggcacgg gtaagatcgg tcgccttgta gccaagcgac tcatggcctt
     3361 tgagtgccgc atccttgcct ttgatatccg acccgacgag cagttacagg ctgagggggt
     3421 agaatatgtg ggttacgaag aactttttcg taaaagcgac atcatcaccc tgcactgtcc
     3481 cctaatgcct gccacctatc atctgatcaa cgaagaaagc cttgcactca tgaagcgtgg
     3541 aatcatcctc gtcaacacaa gtcgtggggc gttggtggat agtcaggctg tgatccgcgc
     3601 acttaaatcg ggacgcattg gcgcgttggg gctcgacgtc tatgaagaag aggcagacct
     3661 ctttttcgaa gacctatcaa atcaggtgat tcaagatgat gttttcatga ggatgctgac
     3721 cttccccaac gtcatcgtca cgggacacca ggcgtttttc acccgaaatg ccatggaggc
     3781 cattgccaag aaaacgctgg agaatgtcac agcctttgaa cgcggcgagg agccacccgg
     3841 ccttctccat taccgcgaag cctttgcgtc ctaacgatgt tggcccactt aatcctgcgg
     3901 taaacttcgc agaacgtcgg tcggcgcata atcgtccgtc agaatgggac cgtttcgcac
     3961 gtaatcgccg ccgggtctcc ccatttcggc gatctcaggg agataaaact gaaacttctt
     4021 ggcagcctgc aaacgctcgg caacttcaag gagctgagcc tttgtcagcg tctttggctt
     4081 tgcataggcc acgacaatca catttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnngagct gagcctttgt cagcgtcttt ggctttgcat aggccacgac aatcacattt
     4261 ccatatcgcc catacgacca ttcgttggca aagaccttgg ctattgtgcg acgctggtaa
     4321 tgataagagg caaatcctgg ttgaagattg ctgacgacga tgcccgtgtc cttcaacaag
     4381 gacttggtta gctccataaa ctcttgagtg gtcaagtgat acggaatata tcccccgcgg
     4441 taagcgtcca ggaaaacgat gtcatattta cgattctctc gggcaaactg tctcatctgt
     4501 acccgcgcat cacgaacata gactttcact cgagcattct cctcaaaacc aaaaaactgc
     4561 ttcgccgcat cggctacaac ggggtccagc tcgatagcgt caaccgtggc atcgggaaaa
     4621 tagtgatgaa tggcccgcgg aagcgtgccc cctcccaaac cgacgaagag aatcgacttc
     4681 ggctcgggac aatgcaaaaa ggcagccatc atcagcttgt aatagtgcaa tgggaaatcc
     4741 aggggatcgt ggatattaat cgccgattct tcaaaatcga ggccagaacg gcggaactga
     4801 agtctgcgga tgccgtcctc ttcgatgacc ctcacataat ggtaaaggga ttccttttcg
     4861 agtaagactt tggtatcggc cgccagtgtt gttccattag gacacggact caaggtccca
     4921 atgacgaatc ccgttaagac gaaaaaaatt atcggtaagc gcgactgttt gaccgcacgt
     4981 ttgtagagca acatctcagg actacagtca cctttgttcg ccacttccag caactttatc
     5041 cacttctcca tatggctcac cgtatgctcc ccaaaatggc tctgtgggct ggtttccccc
     5101 atcataatta aacttcagtc agcacgtttg gatttttgga ttttcaggat gcgtcctcgc
     5161 gccgccatgc aaaggcgcta actatgatta aaaccacgga aagcgccgcc aggaccgcag
     5221 ctcccaccaa caaagaattt cgaatgccgg ttgacaaaat cagataaaag gcggtgtgta
     5281 ggcaaccgag gaaactgccg atcgtggaaa tagcataaag tgtaccggca ctggcccctg
     5341 cccgtgacac actagtggtc aggagccgaa tgcagtaggg cgaaaccatg cccataaggg
     5401 tactgggaag gagaaatagt gccgacgccg cggcgagggc gccgtaccgc tcgttccagc
     5461 caagctcagc gaaccatcca ctgacctcgg cataccagaa ggcgatcggt acgatcgaaa
     5521 tacccgccaa aaggatgata accgccaaca cgtagggcga cggccggcgt gccgaggccc
     5581 atccgccgag ataatagccg atgctcaacg aaagcagaaa cacactaatc acactgcccc
     5641 aaacgaagac cccactacca aaatccgggg aaaggatacg tcctcccagg atttccaagc
     5701 ccatcatcac ccagccacaa acaaaggccg aaaaaaacaa aatgaacctt atgactcgct
     5761 ccatgtttat ctatccgtca atctgggacg atcgcgctaa gtggtgggag ccccgttcat
     5821 atctaagagg gtagctggct cggaggatcg cgtcaatcaa gcggtcgtgg gccctttctg
     5881 ggcacgaggc tattcgtcag ccgatgtcta tcttcaacgg cggccccctc ccaccgcaaa
     5941 atccttcttc atccctcttc tgcaaggccc taacatccaa tctcgtgggc acaaaaaaac
     6001 cggctttcgc cggttataaa ggggatcgtc aacgcgatgc ctttttcggt aaatccatca
     6061 agacttcacg caacgttggc acgctcaggc aattggccca tcattccacg tccgtcttac
     6121 cgatggcctc aggatgaaag acagcgtggc aatcatcgca gctctttgtc atagcggcaa
     6181 gggccttctc agcatctgcc tgattctttg ccttcgcggc cttattaatg gcggccgcag
     6241 catcccgtga atcggcacag aacgtaaacc atttctccgc ttggttcggc tgacttgtct
     6301 cgtcaagatt ggccatgctg gcctgagaaa tggctgcgat caccgcggca tcaaccgcaa
     6361 tggcatccgc ctgtcgctcg aaccgccgca tgtttcgctt caaccgagag ttgaccaagg
     6421 ggacctgttg catgagctcc ttcaatgaag ccactttctc ccacttcaaa gtgctcgcgt
     6481 caccactgct gcttttggcg gcttccagag ccgcaaaggc cgttttcgcc gaggcgtaat
     6541 ccttagctgc cgctgcggaa agctccctgg cggccttgat taacgcaggg gctgccgcct
     6601 ggtatggatt cttctcatca tgcaaaccga gaacgagagc caacacggcg attgtctcag
     6661 catctttgac gacacgagct tgaaattcag cgaagtcatc ttgactctca agagtctcgc
     6721 caatccttga gatgtaatca tcaacagctt tgactaagtc ggctgccggt gcataagctg
     6781 acgccttggg aggcgttgga atatctgccg cataacatgc caacccaaag tgtcccatca
     6841 tcgcggtgca tacacccaaa gcaatggcaa gtcttaatcc catgaagtca ctcctcatcg
     6901 caaatgactg actgctggca acccaacgga gttttacggg aatttctttc tcctcgttat
     6961 tcctccggcg acaactgatc gtaacgttta cgcagtcacc ccgttatttg ttctctacac
     7021 caaaaaccac aactcaccct ttaccgcaaa acacgggggt ttgccccaaa accaattccg
     7081 atctgcctgc gtttctggta tcttgatcaa atgtcgttga aaatcagatc gttgtcaagt
     7141 ctttgctcag ccgaaaactt aattgatagg cgcgaatcgt cgcgtcttcc atgcaagacg
     7201 atcgatgctc atttgtgacg ttgaatttca actcatcaag attaacccgg cttcgggagg
     7261 catccccgaa cgcctggttt tggtccgggt ccagacggac gaaggcatgg atggttgggg
     7321 agaagcccgg ttgccgtggc ggctaggtga attacgtgag cggcgagata tgattctgcc
     7381 gctcgtggcg ggaaaaaacg tgctcgatct tgccgaactc tctcgcctgg aacaattgtc
     7441 cccgaagtta cgtgccgcac tcgagatggc ctgcagggac gccgcgggaa aaattctggg
     7501 ccaaccgctt tgttactttt tggggggaac ttaccgcacg aggattccca cggcacatta
     7561 cgttggcgac actgacgacc ctgatctcct agaccgggga caacgctggc tcgataaagg
     7621 catcgccaac
//
LOCUS       DAOPTR010000032         7609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_8013_length_7609_cov_3.746360, whole genome shotgun sequence.
ACCESSION   DAOPTR010000032 DAOPTR010000000
VERSION     DAOPTR010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7609
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8013_length_7609_cov_3.746360"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..3091
                     /locus_tag="PKI05_01220"
     CDS             80..3091
                     /locus_tag="PKI05_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOA50844.1"
                     /translation="MPSVTGVPTTRITNLFVRQRLLQQIQADQRDIVNLQLQLSTGQR
                     FSVPSEQPIVSLRVIGLQRLLEQKEQVKANLATTQSYLTAADTALSSISSMVAEARAT
                     ALGVLGTTATDTQRAAAALQIQQTIQQLLDAGNQKFRGRYLFSGSLTDLRPFGVTGNN
                     LVAFYGNERLLPSYVDTDLLSENNVLGSQIFGAISSAIEGTADLQPRLRFDTPLAHLN
                     NGAGVDLGSIVISDGTRSAVVDLSSAHTIGDVAMLIRRNSARIPLNVEVTSQGLKIQL
                     NAASGDLSIRDVGNGTTARQLGIYREIGVGTSPIIGSPLNPCLKPTTQLKDILGSPAR
                     AALRFPGSDNDFYLEADSNGDALNGVKIRFIDDPSVTVGSEIVEYDATAKELLIRIDE
                     THSQARDVISAINNAYDAGQLPFFAYLDPTDQELYPGQGLVFPTPAGEWAAVTEGGSG
                     EDFDKTSGLLITNGGQTFAVDFSKAATIEDVLNTLNASSYGLLAEIDESARRINVRTR
                     ISGADFAIGENGGKTASQLGLRTLTADTRLDQLNFGRGVSDYRDQGTTARAIFDPVGT
                     HNRLILQARTAGPEFNGFRLSFYDTGGPPGSEIITYDSVNKQIAIGIVPGSTTAKQIV
                     ELFAATPGARDNFQLRLDDESLDNDGSGLLQVGEVLTSGGSSGGIDFTITRADGVVME
                     IDLSGAVTVQDVIDRINNHPNNPPRVPGGTPWLIARLAQYGNGIELIDESVGTGVLTV
                     SRTPSSTAAIELGLVPVGAEYAKATSPGTIGQANVRSSGANNDLIFRTKSPTASGNGY
                     RIIFEDSGGGPESFTFDPANKIMRFAIQPGVTTANRIIELFSSDPVAPLVFDALLDPT
                     DGNDGTGFVEVTSPSSPPTIAGGQSSQLTGRDVNPLETEGIFTALIRLKDALIRNDVW
                     GAQRAIDLLDEAVLNLNFRRAELGAKQQSLDILNNRLEDEDTQLQSALAADFDADLAE
                     VISSLVAKQSAYQAALQATAKIFRMSLLDYL"
     gene            3332..3943
                     /locus_tag="PKI05_01225"
     CDS             3332..3943
                     /locus_tag="PKI05_01225"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOA50845.1"
                     /translation="MSNDQENLARAKQELRQRLKAIRHSLDPEWVLQASQAIQETLVQ
                     LPEYQSSRSIMSYVSTGKEVETHRLLLRMKRESKVVVIPWCEGDELRLFRWESFDELD
                     RGTLNILEPRPDLRSKETKIFSPHELDLVLVPGLAFDRNGNRLGRGRGYYDRFLKTLP
                     RRVVRIGLAFECQLVERVPVSPNDEKVDLILTEFRTYRVPRID"
     gene            complement(4027..6894)
                     /locus_tag="PKI05_01230"
     CDS             complement(4027..6894)
                     /locus_tag="PKI05_01230"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOA50846.1"
                     /translation="MSKQRCKNTPEHRDLASGPSLVSSGALRVFQVTRHLIANVTGFC
                     LRERSPRRWFLFVAIVLVGVALVVFLDWWITVPDSVSVTYVGRESCYRCHEQECRLWE
                     GSDHDLAMDVATSETVLGNFDDCTFTHIAFSDVWRFSDEELRKILSRLDDETLAVALQ
                     PYEVAHTPETFYPYTPPSGDRSRQRILEVLPEERRSAVERLVTWKKTFARPCDITDAH
                     RKLGDAARDLIQSGQIENPDWAVTSRFFRRNGKFYVLTDDRDGTPREFQVDYVFGVYP
                     LQQYLVTFPDGRVQCLPLAWDVKSRRWFHLYPSDPIPAQDPLHWTRALQNWNYMCADC
                     HTTNLQKNYDVTTNTYRTEWSEIDVSCEACHGPASLHVQLADSWSLFWDRKRGNGLVS
                     FSPQKCDNKTVVDSCAPCHARRRPIASPFPPGEAFLNYYVPELLDGNLYYPDGQILDE
                     DYEYASFLQSLMYRKGVRCADCHDPHTARVKFAEKAKVGEVRQPYADNKLCGQCHLPS
                     KYDTVQHHHHPDSTKPGTHCVECHMPETTYMVVDARRDHSLRIPRPDLTVSLGIPNAC
                     NLCHQDPEKGETPDWAVEWVNKWYGPRKEPSHFAYAFEKGRRLDSSGVIELLAVARRQ
                     DLSAIVRASAVLLLANYGSEAARGAVFAAARDPEPLVRLAAARALQNVAIREDDVPRV
                     QHLLSDPIRAVRVESVPWALNLPPQALSGSAMKALQSAIEEYRTSQLLVSDQPGAHMN
                     LAILAEFLGDFQEQEQKYLDALRIDPLFLPARNNLGMIYARTQRFAEAEEQYRRALKI
                     NPDFVPVRDNLARLYYQMGRFDEAEREFRTILSKYPNRGDIHFALGLLLAELPTRLAD
                     AIEELREAARLLPNESRVLYNTAVALQKAGRASESAEYYEKAYRLGNPSADLLQGYAL
                     CLVELKRCAAALAIADELCQRFPNPESAHFRNAIAERCRSN"
     gene            complement(6921..7574)
                     /locus_tag="PKI05_01235"
     CDS             complement(6921..7574)
                     /locus_tag="PKI05_01235"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOA50847.1"
                     /translation="MVNLNLSPASMRVVKLLVGNTPKSVADLIREMKVTRTAVTEQLN
                     ELVAAGLVERQMQRLPGRGRPRHVYSATPGALLLLLSKNQEMVLPNIWRAIREVGGPK
                     LLGEVIKQVSRWLAEHYRKQITATTPAERVLQLVEILREEGVLVDIQEENGRYTIRQR
                     SCPYIMMYEENRAACLLDQETFALVLGGPVRQVACRHDGAPCCVFEVTKDNPATDAA"
BASE COUNT         1741 a         2169 c         2076 g         1623 t
ORIGIN      
        1 ggaccattcc gacacccata caaagcacga gagaccctcg aaacactgac attagcaaaa
       61 cgagtgggtc ccacgaacta tgcccagcgt caccggtgtt ccgacaacac ggattacgaa
      121 cctctttgtc cgccagcgtc tcttgcagca gattcaggcg gaccagcggg atattgtcaa
      181 tcttcagctt caattaagca cagggcagcg tttttcggta cccagcgagc agcccattgt
      241 gtctttgcgt gtcattggac ttcagcggct attggagcaa aaagaacagg tcaaggccaa
      301 cctcgccaca acgcagtctt atctgaccgc ggccgataca gcgctcagca gtatttcaag
      361 catggttgca gaagccaggg cgacggcgct gggagtgctc ggcacgacag ccaccgacac
      421 ccagcgggca gcggcagccc ttcaaatcca acagacaatc caacagttgc tggatgcagg
      481 aaaccagaag tttcgtgggc gctacctctt ctcgggcagt cttaccgatt tacgcccctt
      541 tggcgtaacg ggcaacaatt tagtggcttt ttatggaaat gaaaggctgc taccttctta
      601 tgttgatacc gacctactaa gcgaaaacaa cgtcctgggc agccaaattt tcggtgccat
      661 ctcctcggcc atcgagggaa ctgccgacct tcagccgcga ctccgttttg atacgccttt
      721 ggcccatctc aacaacgggg caggagtcga tttgggcagt attgtcattt ccgacggaac
      781 ccgcagcgcc gtcgtggatt tgagttcagc ccacaccatt ggcgacgtgg ccatgttaat
      841 tcgccgcaat tcggcccgta ttcccctcaa cgtcgaggtg acttcgcagg ggctgaaaat
      901 ccaactcaat gccgcttcgg gtgatctgtc gattcgtgat gtggggaacg gcaccacggc
      961 ccggcaactg gggatttacc gggagattgg agtcggaacc tcgccgatta tcggctcgcc
     1021 cctgaacccc tgcctcaagc ccacgacgca actgaaagat atcttgggaa gtcccgcgcg
     1081 ggcggccttg cgatttcccg gaagtgataa cgatttctac cttgaagctg actctaatgg
     1141 cgatgccctg aacggcgtca agatccgatt cattgacgat ccgagcgtca cggtcgggtc
     1201 ggagatcgtg gaatacgatg cgacagccaa agaactattg attcgcatcg acgaaaccca
     1261 tagccaggcg cgggatgtca tctcggccat caataacgcc tacgacgccg gccaattgcc
     1321 gtttttcgcc taccttgacc ccactgacca ggaactctac ccggggcaag gtcttgtctt
     1381 cccaacgccc gccggagaat gggccgctgt cacagagggc ggtagtggcg aggacttcga
     1441 caagacgtcc ggactgctca tcaccaacgg cggtcagacg tttgcggtgg atttcagcaa
     1501 ggccgcgact attgaagacg tcctcaatac acttaacgca tcctcgtacg gcttgttggc
     1561 agagatcgat gaatcggcaa ggcggattaa cgtccgtact cgaatcagtg gggccgattt
     1621 tgccatcggg gaaaacggcg gaaagacggc ctcgcagttg ggcctgcgaa ccctgacggc
     1681 cgatacccgc ctcgaccaac tcaatttcgg tcgcggagta agtgactatc gggatcaggg
     1741 tacgacagcc cgagcaatct tcgatccggt gggcacacac aatcgactga ttttacaggc
     1801 gcggacggcc gggcccgaat tcaatggttt tcggctgagt ttctacgata caggtggtcc
     1861 gccgggatcg gaaataatca cttatgacag cgtgaataag caaatcgcta tcgggatcgt
     1921 tcccggttca acgactgcca aacaaatcgt cgagcttttt gccgccacac ccggggcacg
     1981 ggataatttt caacttcgtc tcgatgatga atcgctggat aatgatggct ctgggctact
     2041 ccaagtcggt gaggtcctta cgtcaggcgg tagctctggc ggaattgatt tcaccattac
     2101 gcgcgcggac ggcgttgtga tggaaatcga cctttcagga gccgtcaccg ttcaggatgt
     2161 cattgaccgc attaacaatc atcccaataa tccgccgcgc gtgccgggcg gaacgccctg
     2221 gttgatcgcc cgcttggccc aatatgggaa cgggattgaa cttattgacg aaagcgtggg
     2281 aacaggcgtt ttaacagtca gtcggactcc ctccagcacc gcagccatcg aactgggact
     2341 ggttcccgtc ggggccgaat acgcgaaggc cacaagtcct ggtacaatcg gccaggccaa
     2401 tgtgcggtca tccggtgcca ataatgatct catctttagg acaaaatcgc ccacggcgtc
     2461 gggcaacggc tatcgaatca tctttgaaga ctcgggaggc gggcccgagt cattcacctt
     2521 tgatcccgcc aataagatta tgcggttcgc catccaaccc ggcgtgacga cggcaaaccg
     2581 cattattgag ctattttctt cggatcctgt ggcccccttg gtttttgatg cgctcctcga
     2641 ccccaccgac ggcaatgacg gaaccggctt cgttgaagtc acaagcccaa gtagtccacc
     2701 tactattgcg gggggccaat catcgcaatt aacgggccgc gacgttaacc cgcttgagac
     2761 cgagggaatc ttcaccgccc tcatccgcct taaggatgca ctgatccgta acgatgtgtg
     2821 gggagcccag cgggcgattg atctcttgga tgaagcggtg ctcaatctca attttcgtcg
     2881 ggccgagtta ggcgccaaac aacagagcct tgatattctg aacaatcggc tggaggatga
     2941 agatactcaa ttgcagtcgg ccctggctgc cgatttcgac gcggatttgg ccgaggtgat
     3001 ttcctccctc gtggccaagc agtcggcata ccaggcagca ctccaggcaa ccgcaaagat
     3061 ctttcggatg tccttgttgg attatctcta atcgttgcct cataatctct attggagtct
     3121 aacccaataa gttccccctc tcccgctggg agagggttgg ggtgagggca agcgaacgtc
     3181 aaaatcgatc gagaaaaccg gcagcgattc gagccaccct cacccccgac ccctctcccg
     3241 ctgaacagga gaggggagat tacgggatag gctcctggtc gttcatcaag atcgatcggg
     3301 accgtggtaa ccaaagaaac tctttgtatc aatgagcaat gatcaagaaa acctcgcccg
     3361 ggcgaagcag gaacttcgtc aacgtttgaa ggccattcgc cactctcttg atccggaatg
     3421 ggtccttcag gcctctcagg cgattcagga aacgctggtt caactgccgg aatatcaatc
     3481 gtctcggtcc atcatgagct acgtcagcac cggcaaagag gtcgagacgc accgcctctt
     3541 actgcgaatg aagagggaga gcaaggttgt ggttattccg tggtgcgagg gcgacgagct
     3601 aaggctcttt cgctgggaat cgttcgatga gcttgatcgc ggcacactca atattctcga
     3661 gccccggcct gacttacgat ccaaggagac caaaatcttt agtccgcacg aactcgatct
     3721 cgtcctggta cccggactgg cgtttgaccg aaacgggaac cgcctgggac gtggtcgcgg
     3781 ctattatgat cgctttttga agacgctccc ccgaagggta gtgcggatcg gtttggcctt
     3841 cgagtgccaa ttggtggaac gtgtgcctgt gtcaccaaac gatgagaagg tggatctcat
     3901 attgacagag ttccgaacgt atcgcgtgcc ccgcattgac tgacctcggg ttgtggatgt
     3961 cttccgcagt ctaacttgtc ttacgcaacg cgcttcatgg gaaattttcg cccgcaacct
     4021 gaccgatcag ttactccggc accgctccgc gatcgcattg cggaaatggg cactttccgg
     4081 gttaggaaaa cgctgacaca attcgtcggc gatggccaat gccgcggcac atcgctttaa
     4141 ttccaccaaa cacagggcat acccttgaag caggtccgcc gacggattcc cgagtcggta
     4201 ggccttctca tagtactccg ccgattcgct tgctctgccg gccttttgca aggcaaccgc
     4261 cgtgttgtag agcacccgtg attcattcgg gagcagccgg gccgcctccc ggagctcctc
     4321 gatggcatcg gccaagcgcg tgggaagttc cgccaggagt aaccccaagg cgaaatgaat
     4381 atcgccgcga ttggggtatt tggaaagaat cgttcggaac tcccgctccg cttcgtcaaa
     4441 ccgtcccatt tgatagtaca aacgagcgag attgtctcga acgggaacaa aatctggatt
     4501 gattttcaag gctcggcgat attgctcttc tgcctcggca aaccgttggg ttcgtgcgta
     4561 gatcatcccc aaattgtttc tggccggaag gaaaagggga tcgatccgca gggcatcgag
     4621 gtacttttgt tcctgctcct gaaaatctcc gagaaactcg gccaggatgg ccaaattcat
     4681 atgggcaccc ggctgatcgg aaacaagaag ctggcttgtt cggtattcct caatggccga
     4741 ttgcagggct ttcatagcac tgccgctcag cgcctgcgga ggaagattca acgcccaggg
     4801 gaccgactcg acgcgcaccg ccctgatggg atcactcaag agatgctgaa cacggggaac
     4861 atcgtcctcc cggatcgcca cgttttgcag ggcccgcgca gcagccaagc gaacaagagg
     4921 ttcggggtct cgtgctgcag caaacacggc tccccgggcg gcttcacttc cataattagc
     4981 cagcaaaagc acggcactgg cccgcacaat ggcagagagg tcctgccgcc gtgccaccgc
     5041 cagcaactcg atcacgcccg aggaatcaag ccgtcgccct ttctcgaagg cgtaagcgaa
     5101 atgggacggt tccttgcggg gaccatacca tttgttgacc cattccacag cccaatcggg
     5161 agtttcgcct ttttcgggat cttgatgaca aagattacaa gcgttcggaa tgccgagact
     5221 gaccgtaagg tccggtcgag ggatgcgaag actatgatcg cgccgtgcgt ccacaaccat
     5281 gtaagtcgtc tccggcatgt gacactccac acagtgtgtg cccggcttgg tgctatcggg
     5341 gtgatgatga tgctggaccg tatcgtattt actgggcaag tggcattgtc cgcacaattt
     5401 gttatccgca tatggctgac gaacctcacc caccttagcc ttttcggcaa acttcacccg
     5461 ggccgtgtgg gggtcatgac aatcggcaca gcggactcct ttccgataca taagactttg
     5521 tagaaacgaa gcgtactcat agtcctcatc taggatttga ccatccggat agtacaaatt
     5581 accatcgagg agttccggca cgtagtagtt gaggaaagcc tctcccggtg gaaacgggga
     5641 agcaatgggg cgacgccgcg catgacatgg ggcacaacta tcgacaaccg tcttgttgtc
     5701 gcacttctgt ggcgaaaagc tgaccagccc gttgccgcgt ttccggtccc aaaacagact
     5761 ccacgagtcg gccaactgga catggagact ggccggacca tgacatgctt cgcagcttac
     5821 gtcgatctcg gaccattccg tgcgataagt gttggtcgtt acgtcataat ttttctgcaa
     5881 attcgttgtg tggcagtcgg cgcacatgta gttccagttt tgcaaggccc gcgtccaatg
     5941 cagcggatct tgcgccggaa tggggtcgct cggatagagg tgaaaccatc gtcgggactt
     6001 cacgtcccag gccagcggca agcactggac acgtccgtct ggaaatgtga ccagatactg
     6061 ctgcaacggg taaactccaa aaacataatc cacctggaac tcgcggggcg tgccgtcccg
     6121 gtcatcggtg agaacgtaga atttcccgtt tcttcggaaa aagcggctcg taacggccca
     6181 atccgggttc tcgatttgtc cgctctggat gagatcccgg gcggcgtcac ccagcttgcg
     6241 gtgggcatcg gtgatgtcac atggtcgagc aaacgtcttt ttccacgtga caagacgctc
     6301 aacggcggat cgacgttctt ccggtaagac ctccagaata cgctgccgtg agcggtcacc
     6361 actgggcggg gtataagggt agaacgtctc cggcgtgtgg gcaacttcgt agggttgaag
     6421 agctaccgct agcgtctcgt catcaagccg cgacaggatt tttctcagct cctcatcaga
     6481 aaaccgccac acgtcgctga aggcaatatg ggtaaacgta caatcgtcaa aattgccgag
     6541 cacggtttcc gaagtcgcca cgtccatggc gagatcgtga tcagaccctt cccaaaggcg
     6601 gcactcttgt tcatggcagc ggtagcagct ttcccggccc acgtatgtga cggagaccga
     6661 gtccggcacc gtaatccacc aatcgagaaa caccactaac gccaccccga caagaacaat
     6721 ggccacaaag aggaaccatc tgcgcggcga acgctccctc agacaaaagc cggtaacgtt
     6781 ggcgatgagg tgccgagtga cctgaaaaac gcggagggcg ccactagaaa caaggctagg
     6841 gccagaggcg aggtcgcgat gttccggagt gttcttgcaa cgctgcttac tcacaaggca
     6901 catcacagga aaaataacga ttacgccgca tcggtggcgg ggttgtcttt cgtgacctca
     6961 aacacgcagc aaggagcccc gtcatggcga cacgccactt gccgcaccgg ccctccgaga
     7021 accaacgcga acgtctcttg gtcaaggagg caagcagccc gattctcttc gtacatcatg
     7081 atgtatgggc aactccgctg acgaatggta taacggccat tttcctcctg aatatccacc
     7141 aggacccctt cctcccgaag aatctcgact aactgtaaaa cgcgttcggc cggcgttgtc
     7201 gctgtaattt gcttgcggta atgctcggcc agccatcggc taacctgttt gatgacttcg
     7261 cccaggagct ttggcccacc cacttcgcgg atcgcccgcc aaatgttggg caaaaccatt
     7321 tcctgatttt tcgagaggag gaggagcaag gcacctgggg tcgcggagta aacgtggcgg
     7381 ggtcgtcctc gaccaggaag gcgttgcatc tgccgctcca ccaggccggc ggctaccagt
     7441 tcattgagtt gctcggtaac ggctgtccgc gtgactttca tctcgcggat gaggtcggcc
     7501 acactcttgg gtgtgttccc gaccagcaac ttcactaccc gcatactcgc aggacttaaa
     7561 ttcaaattca ccatctattc cccataaaac ataattataa tcaaaagaa
//
LOCUS       DAOPTR010000033         7546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_8104_length_7546_cov_4.162996, whole genome shotgun sequence.
ACCESSION   DAOPTR010000033 DAOPTR010000000
VERSION     DAOPTR010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8104_length_7546_cov_4.162996"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1327)
                     /locus_tag="PKI05_01240"
     CDS             complement(83..1327)
                     /locus_tag="PKI05_01240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOA50848.1"
                     /translation="MSDAISSLACFPSFQTDAGNLFTVSPIADPVVVPICPEEPRTTT
                     ARVIHVINGEHYSGAERVQDGLALVLPQYGFDVQFVCLKPGRFAKCRRAQGAPVENLP
                     MRSRWDVAVASQIAALAEKTSAVLLHAHTPRSAMITALAARKCRLPWLYHAHSPTLRD
                     SGNWVKNFAVAMMEWWAVRQADHIIAVADSLKRYWQRWGISADRVSVVHNGVPSWPTL
                     PKRPHPGGTWTIGTVALFRPRKGIEILLRSLHQLRREKIPVRLLAVGPFETRKYEYHV
                     KSLAARLGLEDVVSWVGFTPHVDTLLAKMDLFVLPSLFGEGLPMVILEAMALGVPVVA
                     SRVEGVPEVIRDGQDGLCVTPGSVLALTHALRTVISGQVSWQSLREQAYRRQRQCFSL
                     ESMGRATADVYRHILRHWTTRS"
     gene            complement(1363..2769)
                     /locus_tag="PKI05_01245"
     CDS             complement(1363..2769)
                     /locus_tag="PKI05_01245"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HOA50849.1"
                     /translation="MSGSQRVIHQYWSVRELFYHVLDGAVIVGALWCARLTLGGFREE
                     DLLAALLAVIVFHVVAEITGLYRNWRGVSLERELLCAAITWFFTLPALVTLGYFVDHG
                     RIVSRETVLVWAGMGFLLLLVSRSAVRWVLNRLRASGYNTHYYAVVGINELAFRLVES
                     LENSPDLGLKLYGFYDDRPESRTGPVPEEIGQKLGNIEELVEHARQRRVDMIYITFPM
                     RAEDRIRKILAQLSDTTASVYIVPDFFVFQLLHSRWTNIGGLPVVSIYENPFYGIDGF
                     VKRAMDVVLASIFLVLLSPLMVAIAIAIKLTSPGPVFFRQRRYGLDGREIRVWKFRTM
                     TVCEDGQQVTQARRGDPRVTRIGAFLRRTSLDELPQLFNVLKGDMSLVGPRPHATAHN
                     EEYRRLIDGYMLRHKVRPGMTGLAQVHGCRGETETVDKMAKRVEWDLQYIREWSLWMD
                     IKILLQTFWVVLRGQNAY"
     gene            3292..4440
                     /locus_tag="PKI05_01250"
     CDS             3292..4440
                     /locus_tag="PKI05_01250"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOA50850.1"
                     /translation="MRSQQRDSLGAFLSDEYYVLTVPNGEQALRILPQAAPDVVLLDI
                     MMPGLNGYETCRQIKSGPLGPFTQVILVSGKSSTQERLEGYAAGADDYIVKPFDHEEL
                     LAKIGVHLRLKKMVERVWDLNMQLHRFTDNLEALVSQRTAEILETRDLLIFSLAKLAE
                     SRDPETGEHLERMRWYSRLIAEEYIASRPHGDSESENFPQEVLRSAPLHDIGKVGIPD
                     VILLKPGRLSPEESVIMQRHTIIGYEALMAACNKSKAGSFLEIAADIARSHHERYDGK
                     GYPDGLAGNDIPLAARIVSVADVFDALTSPRVYKRAWPPDVARATIERGSGTQFDPDV
                     VAAFLRRWRAIEEIGAPFWNTIPPDRLEPHSLIQTSAPPLLTASLATV"
     gene            complement(4488..4955)
                     /locus_tag="PKI05_01255"
     CDS             complement(4488..4955)
                     /locus_tag="PKI05_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50851.1"
                     /translation="MRLWMPGVVVLSVILIGGCCAPCHRLGMHHYAAPGAVYGEEPGV
                     VAYHAGPLAWLWRLLGVGRGYPCDDCGPRYWGDWGGDRAGCEPCDDYGSWTGMPPVEY
                     RGAAGTYSSSGDKRGGCPECQSSSSSGSTVSQINPRTGRTSVAVQSTKESSTR"
     gene            complement(5165..6721)
                     /gene="amrB"
                     /locus_tag="PKI05_01260"
     CDS             complement(5165..6721)
                     /gene="amrB"
                     /locus_tag="PKI05_01260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOA50852.1"
                     /translation="MNARLSDRIRPPAVAGTFYPAQRERLREMLDQFLAEAPVICGPD
                     RTIRAIIAPHAGYRYSGPVAGVAYKQLAGRDYNTVVLLGPSHFAHFEGGALPDVDAFA
                     TPLGEVAIANKARILAQKEPYAFDPPAVVQAPRGWTTTGTSACTPHTFEHSLEVQLPF
                     LQVILKEFEIVPIVFGRVNVNQAAEAVASILDDRTLIVVSSDLSHYQPYRRACSVDQR
                     TLQAIIQMDFDYLEGLRYEPELCPCGHTGILVLLRLASRFQWEPILLDYRNSGDTSGD
                     RSAVVGYGAVAFCEGRARSVAKANEEEVKAEEINPVASGAVSASGDTEEAPSEARLPQ
                     LTAEEREFLLNLAWKTIRRYVIDRRLPEVEPESVPPRLREPWACFVTLRRRGALRGCI
                     GTLTAERPLYLAVMENARNAARDPRFPPLEDWELTDLTVEISVLSEKRPLDARSPEEI
                     LRKLRPGIDGVILERFGARATFLPQVWEDLPDPVEFLQRLSQKAGLPADAWRQPATRI
                     FTYEVEKFGD"
     gene            7122..>7546
                     /locus_tag="PKI05_01265"
     CDS             7122..>7546
                     /locus_tag="PKI05_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HOA50853.1"
                     /translation="MIHLTQKGCRAVDTGAAQILVPGVVFLPGLGLDRSAGRVEPGSF
                     IAGEENWVALAAVQRMIEPHPPSCFTLFGPTGTGKTHLLGLLCGLWKEIWPHCSVVSS
                     TAAEFGQAFAEALVSRKARQFRRHWEEADFIVIDDLQFLG"
BASE COUNT         1697 a         2306 c         2050 g         1493 t
ORIGIN      
        1 tggttttctc gatcgactcc gacgatcgct tgccctcacc ctaaccctct cccaaaggga
       61 gaggaaattt tgggataggc tcttatgagc gggttgtcca atgccgcaag atgtggcgat
      121 aaacgtccgc cgtagcgcgt cccatgctct ccaaagaaaa gcattgtcgt tgccgccgat
      181 aggcctgttc gcgcaacgat tgccaactga cttgcccaga gatgaccgtt cgcaaggcgt
      241 gcgtcaaggc caacacggaa ccaggcgtca cacaaagccc gtcttggcca tcgcggatga
      301 cctccggaac accctccacc cggcttgcca cgacagggac gcccagggcc atcgcctcga
      361 gaatgaccat cgggaggccc tcgccaaaga ggctgggaag gacaaacagg tccatcttcg
      421 ccagcaaggt atccacatgc ggcgtgaagc ccacccagga gaccacatct tccaggccca
      481 agcgggccgc caggcttttc acgtgatact cgtattttct agtttcgaac ggcccaacgg
      541 ccaagaggcg caccgggatc ttctcacggc gaagctggtg cagcgaacgc aaaaggatct
      601 cgattccttt ccgcggtcgg aagagggcca ccgttccgat tgtccacgtt ccgcccggat
      661 ggggccgttt gggaagggtc ggccaggatg gcacaccatt gtggacgaca ctcacccggt
      721 ccgccgagat accccacctc tgccaatatc gcttgaggct gtccgccacc gcaatgatgt
      781 ggtctgcttg gcggaccgcc caccattcca tcatcgcaac cgcaaagttc ttaacccagt
      841 tgcctgaatc ccgaagcgtg ggactatgag cgtggtagag ccaagggagg cgacactttc
      901 gcgcagcgag cgccgtaatc atcgcggacc gcggcgtatg ggcatggaga agcaccgccg
      961 aggtcttctc tgccaacgcg gcaatctgcg aagcaacggc aacatcccag cgcgaacgca
     1021 tgggaaggtt ctccaccgga gccccttgtg cccggcggca cttggcaaat cggcccggct
     1081 tcaggcagac gaactggacg tcgaagccgt actgtggcag gacgagggcc aaaccatctt
     1141 gaacccgctc ggccccggaa tagtgctccc cattgatgac atggatcacg cgggccgtcg
     1201 tcgtccgcgg ttcttcggga caaatgggta ccaccaccgg gtcggctatc ggcgaaaccg
     1261 tgaagaggtt accggcatcg gtctgaaacg atggaaaaca ggcgagggac gaaatcgcgt
     1321 cactcattgc ggcgtggccc ttttctgaaa acgacgaacc gatcagtaag cattctgacc
     1381 tctcaagacg acccaaaacg tttgcaggag aatcttgata tccatccaca aagaccattc
     1441 gcgaatgtac tggaggtccc attccacgcg cttcgccatt ttgtccacgg tttcggtctc
     1501 cccccggcac ccgtggacct gggcgagacc tgtcatgccg ggcctgactt tatgccgcag
     1561 catgtacccg tcgatcagtc ggcggtactc ctcgttgtgc gccgtggcgt gaggtctcgg
     1621 tcccacaagc gacatatctc ccttcagcac gttgaataat tgcggcagct catcgagcga
     1681 ggtacgacgg agaaaggcac cgattctcgt gacccgtggg tcaccccttc gggcttgcgt
     1741 cacttgctgt ccgtcttcgc acaccgtcat ggtgcggaac ttccatacac ggatttctcg
     1801 cccatccagc ccataacgcc gttgacggaa gaatacaggc ccaggcgatg ttaacttgat
     1861 ggcaatggcg atcgccacca tgaggggcga cagcaacacg aggaaaatgc tcgccaagac
     1921 cacgtccata gcccgcttca caaagccgtc aatgccgtag aatgggtttt cgtagatgct
     1981 gacgacgggc agccccccaa tgttggtcca gcgagaatgg agcaattgaa acacaaagaa
     2041 atcgggcacg atatagacgg acgccgtggt gtcggacagt tgggccagaa tcttccggat
     2101 gcgatcttcc gcccgcatgg gaaaagtaat gtagatcata tcaacgcgtc gctgccgggc
     2161 gtgctcaacg agctcctcga tgttgcccag tttctggccg atttcctcgg ggacaggccc
     2221 cgtccgcgac tccggtcggt cgtcatagaa tccgtagagc ttcaagccca aatcgggtga
     2281 gttctccagg gattccacca ggcgaaaggc caactcgttg atgccgacaa ccgcataata
     2341 atgggtgttg tatccgctgg cccgaagcct gttgaggacc caccggacgg cgcttcgcga
     2401 tacgagtaac agcagaaagc ccatgccggc ccaaacgagc acggtctcgc gggaaacgat
     2461 acgcccatga tcgacaaagt atcccaaagt gacgagcgcc ggcaacgtga agaaccatgt
     2521 gatggcggca caaagcaact ccctttccaa ggaaacgccc cgccaattgc ggtacagccc
     2581 ggtgatctcg gccaccacat gaaacacgat gactgccagc aaggcagcaa ggaggtcctc
     2641 ttcccggaag ccgccgaggg ttagccgagc gcaccaaagg gccccgacga tgacggcccc
     2701 gtccagcacg tgatagaata actcacgcac cgaccagtat tgatgaatga cacgttgcga
     2761 accactcatc gccgccgcct tcgattgtta atgtcacgtg tatcgcaagc aaacacttac
     2821 ttcggaatgt tcttcgccgg gctaaaagac ccgattgaca atcccaatga atatagctca
     2881 attccaggtt gcagaatttc tgacaggttt tccgatcggt ttaggtgcgg cggcaccgga
     2941 tgagaagcct tccgcagcgg cagaagctcg tcgactgttc agcacataat tgccggtttt
     3001 tcccgtccgc agatggctgt gcgtgccgat tgtagcgatt ctatcggctg tgatgggccg
     3061 gtgctgtcgg tcttgccctt ttcctggtct ggtcaccatt acgggtacgc gcgcgattaa
     3121 tgccgccaac ctatgctttt tatgcacaga ttcatcgtgc taggccgaag gcagccgcca
     3181 ctttaggaga acggtccgac caggaactgc cacaactcat cgtggccagg tgaatcctca
     3241 atgggggaag aacatggact cacaaaagcc aaccatcctg gttgttgatg actgcgaagt
     3301 caacagagag attctcttgg agcttttctt tcagacgagt attacgttct gacggtacca
     3361 aatggtgagc aggctttacg aatcttgccg caggcagcgc cagatgtcgt tcttctcgat
     3421 atcatgatgc ccggtctaaa cggttacgag acttgccggc aaatcaaatc agggccgttg
     3481 ggacccttca cccaggttat tctcgtgagc ggtaaaagtt ccacgcagga acgtctcgag
     3541 ggttacgcgg ctggggcgga tgattacatc gtcaaaccgt ttgaccacga agaattactt
     3601 gcaaagatcg gcgttcatct ccgcttgaag aaaatggtcg agcgggtctg ggacttgaac
     3661 atgcaactcc acaggttcac cgacaatctt gaggcgctcg tttcgcagcg aaccgctgag
     3721 atcctggaga cccgcgacct gttaatcttt tctctagcta aattggctga atcacgcgat
     3781 cccgaaacgg gtgaacacct ggagcggatg cgttggtatt cacgacttat cgccgaagaa
     3841 tatattgcca gccgcccaca cggcgactca gaatccgaaa actttcctca ggaggtactc
     3901 cgttctgccc cgcttcacga catcggaaag gtcggaattc ccgatgttat tcttctgaaa
     3961 cccggccgac tctctcccga agaatcggtc atcatgcagc gacatacgat catcggatac
     4021 gaggctctca tggcagcgtg caacaaatcc aaggcagggt ctttcctgga aattgctgcc
     4081 gacatcgcgc gctcccacca cgagcgctat gacgggaaag gctaccccga cggtttggcc
     4141 ggtaatgaca ttccgcttgc cgcccgcatt gtgagcgtgg ccgacgtttt cgacgcactc
     4201 acatcgcccc gggtgtacaa gagggcctgg cccccagacg tggcacgcgc gaccatcgaa
     4261 cgtggatcgg gaacgcagtt tgatccggac gttgtcgccg ccttccttcg ccgctggcgg
     4321 gccatcgaag aaatcggcgc ccctttctgg aatacgattc ccccggatcg tcttgagcct
     4381 cactcgctta tccagacaag cgcacctcct ctcctaacgg cctctctcgc aaccgtctga
     4441 tgacgtggtt ggcccgcggc acaccgccct cggtgcctat cggcttatca ccgagtgctc
     4501 gactctttcg tagactgaac ggctacactt gtgcgtcccg tccgcgggtt aatttggctg
     4561 acagtactgc ccgacgagct tgacgactga cactccggac agccgcctcg cttgtcgccg
     4621 gaactcgagt aggtcccagc ggcaccacga tattcaacgg gcggcatgcc tgtccacgac
     4681 ccatagtcgt cgcacggttc gcacccggca cggtcaccac cccaatcgcc ccagtagcgg
     4741 ggaccacaat cgtcgcaggg atagccccgt ccaacgccga gcaggcgcca taaccatgcc
     4801 aatggcccag catggtacgc gacgacaccg ggctcctcgc cataaacagc ccccggggcg
     4861 gcgtaatgat gcatacccaa acgatgacat ggcgcgcaac atccgccgat gaggattacg
     4921 cttaacacaa ccacgccggg catccacaag cgcatggtcg gtacctcgcc ggtattccta
     4981 agacaaaacg ggagcttgtg ctgacctgtg ggacaaaaac catcctttcg gcagcattcc
     5041 cgaagaatcc gagtcgcatt ctcttcgcaa actgttatcg gttacctttt cgaaaaactc
     5101 ccgaaaatcg gcacatttta cggaatcaga aaagctaaca ccggaagagc cgcccgccgc
     5161 gatcctaatc gccaaatttc tccacctcgt aggtgaaaat tcgtgtcgcg ggttggcgcc
     5221 aagcatcggc aggcaggccc gccttttggg ataagcgctg gaggaactcc accggatcgg
     5281 gaagatcttc ccacacttgg gggaggaacg ttgcccgagc accaaacctc tccaaaatca
     5341 ccccgtcaat gcccggtcgg agttttcgga ggatttcctc gggcgaacgg gcgtccaacg
     5401 ggcgtttttc tgataagaca ctgatttcta ccgtcagatc ggtcaactcc caatcttcca
     5461 aaggagggaa acgcggatca cgggcggcat tgcgagcgtt ttccatcacc gctaggtaaa
     5521 gtggccgctc ggccgtcaag gtcccaatac aacctcgcag ggccccgcgc cgccggagtg
     5581 tcacaaaaca ggcccatggc tcccgtaagc gaggtggaac cgactccggc tcgacttccg
     5641 gtaagcgtcg atcgatgacg tagcggcgga tcgtcttcca ggccaaattc agcaagaatt
     5701 cgcgttcttc ggcagtcagt tgtggcaaac gagcttcgct cggagcttct tccgtatctc
     5761 cactggccga caccgcacca cttgccaccg ggttgatttc ttcggccttc acttcttctt
     5821 catttgcctt ggccacagag cgcgcgcgtc cctcacaaaa agcgacggct ccgtaaccca
     5881 ccacggccga gcgatcaccc gatgtgtcgc cactgttccg ataatcaagc agaatgggtt
     5941 cccattggaa acggctcgcc aaccgaagca agacaagaat gcccgtatgg ccacaaggac
     6001 acaactctgg ctcgtaacgg agaccctcga ggtaatcgaa atccatttgg atgattgctt
     6061 ggagtgtccg ctggtcaaca ctacacgccc gacggtaagg ctggtaatgc gagagatctg
     6121 aactcacgac gatcagcgtc cggtcgtcga gaatactcgc cacggcctcg gcagcctggt
     6181 tgacattcac ccgaccgaaa acgatcggga cgatctcaaa ctctttcaag atgacttgca
     6241 gaaagggaag ctgcacctca agcgagtgct caaatgtatg tggtgtgcag gctgaggtcc
     6301 ccgtggtcgt ccagccccgc ggggcctgaa ccacggccgg aggatcaaag gcatacggtt
     6361 ccttctgcgc caagatcctg gccttgtttg cgatcgccac ttcgccaagc ggagtcgcaa
     6421 aggcgtccac gtctggcagg gcaccgcctt caaaatgggc aaagtgcgac ggaccgagca
     6481 agaccaccgt gttataatcc cggccagcaa gctgcttata ggccacaccg gccaccggac
     6541 cggaatagcg atatcccgcg tggggtgcaa tgatagcgcg gatcgtccgg tcgggtccgc
     6601 aaatgaccgg tgcctcggcc aggaactggt caagcatttc tctgagacgc tcccgttggg
     6661 cgggataaaa ggttcccgcc acagccggcg ggcggattcg atccgaaagt cgagcgttca
     6721 cggcatggac acctgagacc ctgaggaggt cctgcgcggt ctttttcacc ccgtccattc
     6781 tacgccaacc acccaggaat acaaaccacc cacattaaaa cctatggatt gaacaaagct
     6841 tccccgtagg cgacgcgcac acattcacgg tctcaacgag gcgctttcgc ttccgtgatt
     6901 gctcagtatc tcagcaaaac ccatagggtg gccttgccgt agcgggagct acggagcaaa
     6961 atggcaggcc tattgggctg aaagtcaaac caagcaaatg gcacaccaat ggctgtagag
     7021 tccacccccg ctgcgtcttc ccaggaaaac ctcccatctt cgctcgtcaa acaatggaga
     7081 agcaggtatg ataaatccaa ttacactggg tggaaggatg gttgattcac ctcacccaaa
     7141 agggctgtcg cgccgtggat actggagccg cccagatact cgtgcctgga gttgtttttc
     7201 tgcccggcct tggtctcgac cggtccgccg gccgtgtcga gccgggatcg tttattgcgg
     7261 gcgaagagaa ctgggtagcc cttgccgctg tccaaaggat gattgagccc cacccaccca
     7321 gttgcttcac actcttcggt ccgacgggga cgggtaaaac gcacctgttg ggccttcttt
     7381 gcggcctttg gaaggaaatc tggccacact gctccgttgt ttcatcaaca gccgccgagt
     7441 tcgggcaggc cttcgccgag gccctcgttt cccgcaaagc ccgtcaattc cgccgccatt
     7501 gggaagaggc ggacttcatc gtcatcgacg atctccaatt cctcgg
//
LOCUS       DAOPTR010000034         7465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_8209_length_7465_cov_3.726181, whole genome shotgun sequence.
ACCESSION   DAOPTR010000034 DAOPTR010000000
VERSION     DAOPTR010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7465
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8209_length_7465_cov_3.726181"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(380..1288)
                     /locus_tag="PKI05_01270"
     CDS             complement(380..1288)
                     /locus_tag="PKI05_01270"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOA50854.1"
                     /translation="MDGTVLGASENPSFEPFVTDDYELIDFGDSRRLERFGSVILDRP
                     SPAAEGPPLYPDRWSQAHARFERTRGNRGRWVDRCHVPERWTIRWGPLVLELKRNESG
                     HVGLFPEQVVNWSWLYHHITQSEKVGPVLNLFAYTGGSTLAAALAGAEVVHVDAARNV
                     VAWAKRNAVLSGLENLPIHWIVEDARKFLRREVRRERRYWGIILDPPTYGHGPRGESF
                     QLEKDIVGLLEAVFALLDWDRGFLLMTCHTPGYGTQKLTRLFHDLRPDLWQRGQITVR
                     MMTIPSSSGRPLPSGMVVRWQSSPPR"
     gene            complement(1311..1574)
                     /locus_tag="PKI05_01275"
     CDS             complement(1311..1574)
                     /locus_tag="PKI05_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50855.1"
                     /translation="MAEIPVGRVTHYFSHLGVAAIEITHGTLSVGDTIHIKGHTSDFY
                     QPVTSMQIEHQTVTQAQAGQSVGIKVVEHAREHDVVYKVVPEE"
     gene            2394..3626
                     /locus_tag="PKI05_01280"
     CDS             2394..3626
                     /locus_tag="PKI05_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50856.1"
                     /translation="MDCFRFLHTGELWLDRPIRVRGGLPPSLMARVLEGPRRTWERIC
                     QLALREDVDLVVLSGNLVDGSRASPADLDAVLAGCETLAQNDIAAIWMAGKAEEQGPW
                     PAELALPDTVHVFSGEEVQQRQVRTRKGTIDVLVWPTSYPWEASREAFLPHQPGQFVL
                     GLARCQSLEELPNIIGVSYWALGGPVPRTDSLPSTVAHAAGPPLIRDADDPALPTVTI
                     AEWNINRRIALREVSVAAIRYATVVLLISPETQWAGLSSQLVQELESRRASSDADWLI
                     AIKLQGPVEALIRWKREGLDAQLLEFVQSRYLQEPPYLWPTDVVWDPVDLGPDEWLKE
                     TSFRGEFLRQVHAIYENLVIGQTDAVAALAGPISPGQAVDAIVPIWEEILRQAAFEGL
                     EALSHVSASVKGEASACS"
     gene            3617..7129
                     /locus_tag="PKI05_01285"
     CDS             3617..7129
                     /locus_tag="PKI05_01285"
                     /inference="COORDINATES: protein motif:HMM:NF024904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOA50857.1"
                     /translation="MLLKRLAIDRFGIWHNWEVEDIPRGLAVFFGPNETGKSTILEFV
                     RGMFFGLAARSRFLLGEQGDIGGTLVLEHLGREVRLSRHWSQDEGERVQVVVDGQPVL
                     PEELMRVLCPVDEATFNAVFALGLEDLAYLRTLEEGETAKLLYELSLGADRAVLWRVL
                     TNLHQRLAQDQDDTELKELEAERARLVRTTEEAGLETRQYVRWRVTQRKLQAERQTVM
                     QRLDSQQREQQRCEAVLDLLPKWQQYREMRLQWKRLGPSRRISEKALRKLDRWKERMT
                     AAKHNLAEAREALGQYDEALAGLPVDPVVLEHAAEIEALRLHRDEMVACKERLEELNR
                     RQQELLQARSALWEGLASVTQIGNISTPNDGLPQADQRGTAQDWSALRGWIRRWQKAV
                     LERDRLAEEDQRQRAAQNALKEQVEAALQKHGVADAQVALSQYSEQVTGLRRVRQLAE
                     QQAAVKQRVEQVTTEYHRQVAQSLPSWQTWAGVGSLFIPGLTLVFLSLLALLGGPGAG
                     NLGLVTLLLGAAMTGGGIGLKFYHEHRQRVNLENLKKDLETLTTELQSVSQEYQRLAA
                     EQNVSQADLDQLISQLEGRIRELEPVAVLQAKFAEMTQPGQTHRETLHRLEQKERAIR
                     AKFNELAHRLGLPSADDVTQALQIWEVGHRLRALDRRLTVVTAKLKKEEERLAAWQKR
                     VLRLARLCGRSDETDDVVQVLDGLVRHYEIHLENQRRRQDLLRTAKQTKQKVHRAKRR
                     LKKIALRYRATLRQLGVRNPQEATALRQTWEQAQTLRRQAGEIHRELKAALAAAGIAE
                     DVPLARLFQETKAKHAELIQAMEATKEELHALEEKQAHVEAELARLVQSRDVDQAKLA
                     LAALEHQIVRRVRRQQTSRLLAELLERVRQHYELENQPETLRRASVFLAQMTEGRYVR
                     VWTPISERVLLVEDATGKNWRVDELSRGTREQLFLSFRLAIVERSAEQGIRLPLVLDD
                     VLVNFDFQRAAAACRTLRAFAEASHQVLLLTCHDHIARLCTEMSVPVAHLETTGGGKA
                     TPPFFSGYFPPLKTADTPIAPTTPETPRLSGSQEEKTVADGREKVLSQEPSRSRRRRK
                     QTPNGKAVETEKVLGDHKQPGEMASPSAGQVFFQSNCTWLTEAEPYADVNEEGNPQGE
                     AKGDRSSAEPVGNHRAKQRRARAA"
     gene            complement(7169..>7465)
                     /locus_tag="PKI05_01290"
     CDS             complement(7169..>7465)
                     /locus_tag="PKI05_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOA50858.1"
                     /translation="GGIRENLARIVPPGVRIVIDRDAWPVPPIFRWIQKLGETDDAEM
                     ENVFNLGLGMILVVSDYYAASIRKQLEDLGVPCWQIGRAEAADESGVVWAQSSA"
BASE COUNT         1587 a         2167 c         2243 g         1468 t
ORIGIN      
        1 ttgccttggc ggagggattg gcgggctagg tcttccgggg cctcggcaag cgttgcattt
       61 gccacaagcc cataggcgaa caaaaggccg acagcgagga gtaaacccat tggcccgagg
      121 ccgatctttc gacaagtgcc gcgttttaac cccatcgaca ttcggtcgtg tgcgtcttgc
      181 ggtccgagac aacttggctt tttacagtgc ttcttcatta tgacgggaag aaaatcgtcg
      241 ctcaattgat gccgcgactc agcattttcc tattgcaatg ggctatcctc gctaagcagc
      301 cccgttttac gttgaggcgc atagcgcggc gatccgttac ccccttcttg ctacggtggc
      361 tcgggcttgc tgggagtgct taccgtgggg ggctgctttg ccaacggaca accatcccgc
      421 taggcaacgg tcgtccgctg ctgctgggga tcgtcatcat ccgcacggtg atctgacctc
      481 gctgccaaag gtcaggccgc aaatcgtgaa agagacgcgt caatttctgc gtgccgtagc
      541 ccggcgtgtg acatgtcatg agaaggaatc cgcggtccca atcgagcaag gcaaagaccg
      601 cttccagaag tcccacaatg tccttttcca attgaaacga ctcaccccgc ggaccatggc
      661 cgtatgtcgg cgggtcaaga atgatccccc aataccgtcg ctcgcggcgg acctcccggc
      721 gaagaaattt gcgggcatct tcgacaatcc aatgaattgg cagattctcc aatccggaaa
      781 gcacggcatt tcgttttgcc caggcgacca cattgcgggc agcatcgacg tggacaacct
      841 cggcccctgc caaggccgcc gccagggtgc tcccgcctgt gtaagcgaag agattcaaca
      901 ccggccccac cttctcactt tgggtaatat ggtgataaag ccagctccag ttgaccacct
      961 gttccggaaa aagtcccacg tggcccgatt cattccgctt tagctccagg acaagcggcc
     1021 cccagcggat cgtccatcgc tccggcacgt ggcaacggtc aacccaccgg ccacgattac
     1081 cacgagtgcg ctcaaatcgg gcatgggcct gggaccagcg gtcggggtaa aggggcggac
     1141 cttccgccgc cgggctgggc cgatcgagga tcacactccc aaagcgttcc agccgccgac
     1201 tgtcaccgaa gtcgatcaac tcgtagtcat ccgtgacaaa gggctcgaag gacgggtttt
     1261 ccgaagcacc caacaccgtt ccgtccacga tcgttcctcg agctggccaa ttattcctcg
     1321 ggcacgactt tgtacacgac gtcgtgttct cgcgcatgct caacaacttt tatgccgacg
     1381 gactgtccag cctgggcctg tgtcaccgtt tgatgttcga tctgcataga agtcaccggc
     1441 tgatagaagt cactggtatg ccccttaatg tggatagtgt cgcccaccga gagcgttccg
     1501 tgggtaattt cgatggcggc cacacccaga tggctgaagt agtgcgtcac acgtccgacg
     1561 gggatttcgg ccatggcgaa ggctcctttc tggcaaaatg gcacccgttc cgtgtgaccg
     1621 gattgaaggt ccacccaaca accgcaatca cacgccacag atcgcccaaa cgcgaacaac
     1681 gtcacatcca aagccgctcc gcaacgcggg cagagaattg ccatggcaca aaccctttgg
     1741 cttccagtat ggcggatgtc atgacgaaag acaagcgcgt ggatcttggc aggtcaacgc
     1801 cacagtggtc acgacgtgcc acgccggcgg gcgtccctcc agatttgatc gagcgcggcg
     1861 caccaacgtt gagaattcca catgacgtga gccaggttgg ctcaataagg gcctatcgca
     1921 taatctcccc tcgcccgttc ggcgggagag gggtcggggg taagggtggc tcgaatcgct
     1981 gccggttttc tcaattgact ccgacggtcg ctcgccctca ccccaaccct ctcccggagg
     2041 gagagggggg cttggggtag atcttaagaa ggggaagact tgtgcttcgc ccgcactggg
     2101 aatggaacac atagagtaga tcaaattgga acacacacgg tccatgggga acaagcggtt
     2161 taggctggcg gcttgtatcc cagatcgtcc acggcgcgtc agatggccca actcgcttgc
     2221 gataaggggc taaggccaat tgccgcgtca aaacgatatg gcgtcattcc ggcgttttgg
     2281 ctacatttgg cacgttgcgt gttggtcaca aggtgcgcca gcatctggaa aatgggggat
     2341 ttggcaagaa tagaaagaat ttcagcaaca gaaaaagttt caacgaggcc agcgtggatt
     2401 gttttcgctt tttgcacacg ggcgagcttt ggttggatcg gcctatccgc gttcgcgggg
     2461 gtctgccacc ctcgctgatg gcccgcgtgc ttgaaggtcc ccggcggacc tgggaacgca
     2521 tctgccaact tgccttgcgt gaggatgtcg atctggtggt gctttccggc aatttggtag
     2581 atggctcgcg tgcctcgccc gccgatctgg acgccgtctt ggccggttgt gagacgctcg
     2641 ctcaaaacga cattgctgct atttggatgg ccgggaaagc ggaagagcaa ggcccctggc
     2701 cggccgagtt ggccctgccg gacacggtcc acgtattctc gggagaggag gtccagcagc
     2761 ggcaagtccg gacacggaag ggcacgatcg atgtgcttgt ctggcctacc tcctatccgt
     2821 gggaggccag ccgcgaggcc ttccttcccc atcagccggg ccaattcgtg ctgggactag
     2881 ctcgctgcca gtcgctggag gaattgccca atatcatcgg cgtcagttat tgggccttag
     2941 gtggtccagt gccacggaca gactcattgc cctccaccgt ggcgcacgct gcgggtcccc
     3001 cgctgattcg agatgccgac gatcccgcgc tccccaccgt gaccatcgcc gagtggaata
     3061 ttaaccgccg gatcgccctt cgcgaggttt ctgtggcggc aatccgttac gcgacggtgg
     3121 tgttgctgat ttcgccggag acacaatggg cgggcttgtc ttcccagttg gttcaagaat
     3181 tggaatcccg tcgggccagc agcgatgccg attggctcat tgccatcaaa cttcaagggc
     3241 cggtcgaggc cctcattcgc tggaagcggg aagggttgga cgctcaactt ttggagtttg
     3301 ttcagagccg ctacctccaa gagccgccgt acctttggcc gacggatgtc gtctgggacc
     3361 ccgtcgatct aggaccagac gagtggctta aggaaacgag ctttcgtggg gagtttctcc
     3421 gccaggtgca tgcgatttac gaaaaccttg tcataggaca gacggacgcg gtggccgctt
     3481 tggcggggcc gatttcgcca ggccaagccg tcgatgccat cgttccgata tgggaggaaa
     3541 tacttcgcca ggcggcgttt gagggactgg aggccctgtc ccatgtgagt gcctcagtaa
     3601 agggagaagc gtcggcgtgc tcctgaagcg tttagccatc gaccgatttg ggatttggca
     3661 caactgggag gtggaagaca tccccagggg tttggcggtc ttttttggac caaacgaaac
     3721 cggcaaatcg acaatcctgg aattcgtgcg ggggatgttt ttcggcttgg cggcgcggtc
     3781 ccgctttctc ctgggcgaac aaggtgacat tgggggaacc ctggtcctcg agcatctcgg
     3841 ccgcgaagtc cgcctctcgc gacactggtc ccaggatgag ggagagaggg tccaggttgt
     3901 cgtggacggc caacccgtct tgcctgagga actcatgcgg gtcctttgcc ccgtcgatga
     3961 ggccacattt aacgcagtat ttgccttggg gctggaagac ctcgcttatc ttcgtaccct
     4021 agaggaagga gaaaccgcca agctcctcta tgaacttagt ctcggcgcgg atcgggcggt
     4081 cctatggcga gtcctcacca atcttcacca gcggcttgcc caggaccagg acgacacgga
     4141 actcaaagag ctggaagcgg aacgtgcgcg gctcgttcgt actaccgagg aagcggggct
     4201 ggagacacgg caatacgtgc ggtggcgggt cacgcagcgg aagctccagg cggaacgaca
     4261 gaccgtcatg caacggcttg actcccagca acgggagcaa cagcgctgcg aggcggttct
     4321 tgatcttctc ccaaagtggc agcaatacag ggaaatgcgg ctgcaatgga aacggctggg
     4381 gccgtcgcga cggatttcag aaaaggcctt acgaaagctc gaccgttgga aagaacgaat
     4441 gactgcggcc aagcacaacc ttgccgaggc ccgcgaggcg ctcggccaat acgatgaggc
     4501 cctcgccggc ttgccggttg atcccgtggt tcttgagcat gcggccgaga ttgaggccct
     4561 ccgtctgcac cgcgatgaga tggtcgcctg caaggagcga ctagaagaac tgaaccgacg
     4621 acaacaagaa ctccttcagg ctcgatcggc attgtgggaa ggtttggcaa gcgttaccca
     4681 gatcgggaac atctccacgc cgaatgatgg cttgccgcaa gcggatcagc ggggcaccgc
     4741 gcaggattgg tccgccctcc gtgggtggat caggcggtgg caaaaagcgg tcctggaaag
     4801 ggatcgactg gccgaagagg atcagcggca acgcgccgcg caaaacgcac tcaaggagca
     4861 ggtggaagcc gcgctgcaaa aacacggtgt ggccgacgcc caggtcgcct tgtcacaata
     4921 cagcgagcag gtgaccggcc tgcgacgggt ccgacaactc gctgaacagc aggccgccgt
     4981 aaaacagcgg gtggaacaag tcaccacgga atatcatcgg caggtggccc aatcgcttcc
     5041 ctcgtggcag acatgggccg gtgtggggag cctctttatt cccgggctta cgctggtctt
     5101 cctgtcgcta ttggcccttc tcggtggacc tggagcggga aacctggggt tggtcacgct
     5161 gctcctcggc gcggcgatga cgggcggggg tattggactg aagttttacc acgaacatcg
     5221 ccagcgggtt aacctggaga atttgaaaaa ggatttggag accctaacta ccgagttgca
     5281 gtccgtatct caggaatacc aacggctggc cgctgagcaa aatgtctctc aggcggacct
     5341 ggatcaactg atctcccaac tggaagggcg catccgtgag ctggaacccg tcgccgtcct
     5401 tcaggcgaaa tttgccgaga tgacccaacc ggggcaaaca caccgcgaga ccctccacag
     5461 gctggaacaa aaagagcggg caatccgtgc caagtttaac gaattggccc atcgtttggg
     5521 tttaccttcg gcggacgatg tgacccaagc gttgcaaatt tgggaagtgg ggcatcggct
     5581 gcgagccctc gatcgccgcc tgacagttgt tacggcaaaa ctgaagaagg aggaggaacg
     5641 cctcgctgcc tggcaaaaac gggtcttacg actggcccga ctttgtgggc gctcggatga
     5701 gacggacgac gtggtccagg tcctcgatgg ccttgtgcgt cattatgaaa tccatttgga
     5761 aaaccagcgc cgtcggcaag acctgcttcg gacggccaaa cagaccaaac agaaggttca
     5821 ccgcgcgaag agacgcctca agaagattgc ccttcgttat cgggcgacac ttcggcaatt
     5881 gggcgtgcgg aacccccaag aggccacggc ccttcgccaa acttgggagc aggcccaaac
     5941 cctgcggcga caggccggcg aaatccatcg cgaattgaag gccgcccttg ctgccgctgg
     6001 gattgccgaa gatgtcccgc ttgctcgcct ctttcaagag acaaaggcca aacatgccga
     6061 gctgatccag gccatggagg ctactaagga agaactccat gccctggagg aaaagcaggc
     6121 gcacgtagag gcggaacttg ccagacttgt ccaatcgcgc gacgtggatc aggccaagtt
     6181 ggctctggcc gccttggaac accaaatcgt ccgccgtgtc cgaagacaac aaacttctcg
     6241 cctccttgcg gaactgctgg aacgagttcg ccagcattat gaactggaga accagcccga
     6301 gacgttgcga agggcctctg tctttctcgc ccaaatgacc gagggacgtt acgtacgggt
     6361 ttggacgcct atcagcgaac gcgtccttct cgtggaagat gccactggca agaattggcg
     6421 tgtcgatgag ctgtcgcggg gaacgcggga gcaactcttc ctcagctttc gcctggccat
     6481 tgtggagcgc agcgccgagc aaggaattcg gttgccgctg gttctggatg atgttctggt
     6541 caatttcgat tttcaacgtg cggcggcggc ctgtcgcaca ttgcgggcgt ttgccgaggc
     6601 atctcaccag gtccttcttt taacctgtca tgatcacatt gcgcgattgt gcaccgaaat
     6661 gtcggtcccc gtcgcccacc tcgaaacaac cggcggtggc aaggctactc cgcctttttt
     6721 tagcggatac ttccctcccc tgaagacggc tgacacgccg atcgctccca caacacctga
     6781 aacgccccgt ttaagcggtt cacaggagga aaaaacagtt gccgacggtc gagagaaagt
     6841 gttgtcacag gagccatcgc ggtcacgccg gcggaggaag cagaccccta acggaaaagc
     6901 ggtcgaaacc gaaaaggtgc tcggcgatca caagcaacct ggcgaaatgg cttccccttc
     6961 cgccggtcag gtcttcttcc aaagcaactg tacctggttg acggaggccg aaccctacgc
     7021 cgatgttaac gaggagggaa atccccaggg tgaggcgaaa ggggatcgct cgtcggcaga
     7081 accagtgggg aatcatcgag caaagcagcg ccgggcgcgt gcagcgtgat gacgtttttg
     7141 cgacaatacc ttcacggtcg catctgtgct acgcggagct ctgggcccaa acaacgcccg
     7201 actcatcggc ggcctcggcc cgaccaattt gccaacacgg cacccccagg tcttccaatt
     7261 gcttgcggat actggcagcg taatagtcgc tgaccacgag gatcataccc aagccgaggt
     7321 taaagacgtt ctccatttcg gcatcgtcag tctcgcccaa tttttggatc cagcggaaga
     7381 tgggcggcac tggccaggcg tcccgatcga tgacgattcg cacgcccggc gggacgattc
     7441 tggcaagatt ttcccgaatc ccccc
//
LOCUS       DAOPTR010000035         7407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_8282_length_7407_cov_3.391594, whole genome shotgun sequence.
ACCESSION   DAOPTR010000035 DAOPTR010000000
VERSION     DAOPTR010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7407
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8282_length_7407_cov_3.391594"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2316
                     /locus_tag="PKI05_01295"
     CDS             <1..2316
                     /locus_tag="PKI05_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50859.1"
                     /translation="AWGLPMIYLHERDEAIFPGLEWLVKDELSSDTLDIAEDHLDRIR
                     YVVHPNMITIPAVGIHASWGTVGLMWYLQPYGQLLQPAFAFASPDRFNHQRSHLMELF
                     LPPVPKYTPVNSRVAEKPYRLEPGKSLILRANLWMDGEASDALAVVEEYVKRHKIAPA
                     HPLPHTSLPGEIIFSARAYLESLWDAETRKWWTTKGNAILSHQALPPDFAADLALARM
                     LTGDQELKKRCEERLSEVCQVLGLPPRLDALRIGGRADRVWANAGRVAALLASRKEDG
                     SWRFDADRPGSGPFVGMDYRELGPHDALEVGTCARNAYEVLAFARITGDHGLYQEMVK
                     TLELMERFRVPRAAQVWEVPVHTPDILAAADAVDAYLEAYQLSGEKRWLEDAVLWAKR
                     GLPFVYLWNDPEKPYLQGASIPVFGATWYRGSWFGRPVQWNGLRYANALLKLAQYDQS
                     LPWKEVATILIHSAVHQQDLEGENVALWPDNISAITGEKCPWVFAPRQIIGCINRLLG
                     REEEPQTIYLKLGDRSVPLSSLGKIGRPQVEGNALTFTVQFPPGEVGPVLIANVAAPQ
                     EVLINGQPVPQNERPHLVEGSSWCYDSGMALLTIQVGLSGPAEVTVKPLNVRTVDRIP
                     RIVNVIRFYFDEGMEGWMAAHDVADLQVKAGQLVGRIVGPDPYIVRPHMRVPGAKGDT
                     IIIRISTTAGSMAQLFWATESSPSFDEEKSLRFQVQPGETVNEYRLPLGDHPSWNGQT
                     VVGLRLDPGGGTTEGSFVVDAIEGVTASGQR"
     gene            2694..4082
                     /locus_tag="PKI05_01300"
     CDS             2694..4082
                     /locus_tag="PKI05_01300"
                     /inference="COORDINATES: protein motif:HMM:NF019269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1598 domain-containing protein"
                     /protein_id="HOA50860.1"
                     /translation="MHRSAYRCLVVASLALGFGFLSWGTSAWAQQQDDNQMAGVIVSP
                     DGVLTRQMISDPTGQIMRERVMAARRSLPPNVARFSTLRKVSLPRLEKAIVANHGVVT
                     DEMKYLAGLLRIRYVFLYPESGDIVLAGPAEGWVETPTGRVVGINTGRPVLQLQDLVT
                     ALRAYPPDGEGVDVIGCSIDPTQEGLARMQEFLRSLGSTFPPGQEAQVAEYMVNGLKK
                     SLGVQEVTILGVPPETHFAHVLVEADYRMKLIGIGLERPPVRLVSFVERAASSAVSPN
                     QLFRWYFVPDYQCLRVTEDGTALELVGEGVKLVGEDELVTASGERKAASAASAASRAF
                     TTSFTRHYPELAAKVPVYAELRNVIDMAIVAAYIKQQDFYNQANWKMEFLGREENFAV
                     KTYPAPKKAETAITAVIRPGRVSTPIGGGVHIEAQQALDYENLLKDEKGEVQKAKASV
                     KVDLPEGVWWWD"
     gene            complement(4092..5678)
                     /locus_tag="PKI05_01305"
     CDS             complement(4092..5678)
                     /locus_tag="PKI05_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photolyase"
                     /protein_id="HOA50861.1"
                     /translation="MRGNRLIASPATAFAEAASQILSGKIWGGKVPEERIRVIAEGTP
                     HQSPNVVLYWMHINRRWSWNYAVDRALEWVADREKDLTVLETLPFHQTLTDRHAVFVM
                     EGMREHESLVERGPFLYRAQSRKFGRIAVEYLYSLFQTCDVIIVDDHPDREYRAFLES
                     LASRTRARIEAVDSSGLIPFRSVSRYYPTARGFRTLVQRLLPDTLCQLPDPRPLPERI
                     PFRTKKPSHPFWNAWREDHEVLLNPRQGVKELPLDHSAGPVATKGGPTAARNRWRQFL
                     ETALEHYHEWRNHPDENGVSGLAPYLHFGFISPFEMIVDLLKRATEMGWSFDRPSQEA
                     DAGTWWNLPPSIAQFLDQLVVWRELGLNLCLRCDDYDRFESLPDWAKKTLTAHASDRR
                     PITYTLEQLENAQTHDRLWNAAQNELREEGRLHNYLRMLWGKKILEWTPDPQTALEWM
                     IYLNNKYGLDGSDPNSYTGISWIFGRYDRPWGPDRPIFGLVRYMSTDSARRKIRLKKY
                     LERYAPEMKETQVTTEDVKD"
     gene            complement(5764..>7407)
                     /locus_tag="PKI05_01310"
     CDS             complement(5764..>7407)
                     /locus_tag="PKI05_01310"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA50862.1"
                     /translation="GNAVRTVSGSQELSADDTKAAKTVSKGRGFSSLGDTARLVSFLP
                     NLLTKEVLAMARQVTPRREFLQEAILASLGGIGLGAGLPVVAAEQGPQSAGAAAPTSP
                     NDKLQVAVLGVRGRGQDHASAYASRPDCHVLYLCDADRDVGQTYVERFAARFGYKPKF
                     AQDLRDVFADPAVDLVSIATPNHWHALASIWAIQAGKHVYVEKPVSHNVSEGRRIVQA
                     AQKYNKLCQGGTQRRSEGRYRAAAEAIKAGKIGKVKVIRCVMYRPRSPIGPPGDYPIP
                     PGVDYNLWAGPAPMAKVTRKSFHYDWHWFWDYGNGEIGNNCIHCVDTARIIVPFKTLG
                     KWVISYGGRLGFNDAGETPNVQVAVHDLGGITLVQEVRNHKTNPPFLNGAVLIVGEEG
                     YIASSFGPNVVVYDKQGQPRENLTGPNVDNFGNFVEAVKAGKQEMLNAPVEETHISTA
                     LTHLANISYLLGEKAGHKELVAALEKLQVHEDCVATLNAIEEHLKENGIDLEKEPLTL
                     GALLPVDAANECFIDRPDANKLLTREYREPFVVPTVDKL"
BASE COUNT         1567 a         2091 c         2103 g         1646 t
ORIGIN      
        1 gcttgggggt tacccatgat ctatctccac gagcgagacg aggccatttt ccccggtttg
       61 gagtggcttg tgaaggatga actctccagc gacacattgg acattgcgga agaccatctt
      121 gaccgaattc gctatgttgt ccatcccaac atgatcacca ttcccgccgt gggcattcac
      181 gcttcatggg ggactgttgg attgatgtgg tatcttcaac cctacggaca attgttgcaa
      241 ccggcgtttg cctttgcctc acccgatcgt ttcaaccacc agcggagcca cctgatggaa
      301 ctctttctgc cgccagtgcc gaaatatacg cctgtcaact ctcgggtggc ggaaaaacca
      361 tatcgcctgg aaccaggtaa gtcgctcatc ttgcgcgcca atctttggat ggacggcgaa
      421 gcgtcagacg ccctggcagt tgttgaagag tacgtcaaaa gacacaagat tgcgccggcc
      481 catcccctgc cacacacctc gttaccaggc gagattatct tttctgcgcg ggcgtacctc
      541 gaatccctgt gggacgccga aacccgcaag tggtggacca ccaaaggcaa cgccatcttg
      601 tcgcaccagg ccctgccacc ggactttgcc gccgatcttg ccctggcccg catgcttact
      661 ggtgatcaag aattgaaaaa acgctgcgag gagcggctct ccgaggtctg tcaggtattg
      721 ggcttgccac cccgtttgga cgccttgcgg attgggggac gtgccgaccg cgtttgggcc
      781 aatgcgggga gagttgccgc cctcctggcc tcacgcaaag aagacggaag ctggaggttt
      841 gacgctgacc gacccggcag tgggcctttt gtagggatgg attatcgtga gctgggtccc
      901 cacgatgccc tcgaagtggg aacctgtgcc cgtaatgcct atgaggtcct cgcctttgcc
      961 cgtatcacag gggaccatgg cctttatcag gagatggtga aaacactgga gttgatggag
     1021 cggttccgtg tgcctcgggc cgcacaggtc tgggaggtcc ccgtccatac cccggatatc
     1081 ttggccgctg ccgatgccgt ggatgcttat ctggaggcct accagctcag cggggaaaag
     1141 cgttggttag aagatgcggt gctttgggcg aaacgcggcc tgccgttcgt ctatttgtgg
     1201 aatgatccgg aaaaacccta tctccaagga gccagcatcc cggtgtttgg ggccacatgg
     1261 taccgcggct catggtttgg acggcctgtc caatggaacg gcttacgata tgctaacgcc
     1321 ctcctgaaac tggcccaata cgatcaaagc cttccgtgga aggaggttgc cacgatcctc
     1381 atccacagtg ccgtccatca gcaagacctg gagggagaaa atgtggccct ttggccggat
     1441 aacatcagcg ccatcacggg cgagaaatgc ccttgggtct tcgccccgcg gcagatcatc
     1501 ggctgcatca acaggctgct tggccgggaa gaggagccac aaacgattta ccttaaactc
     1561 ggcgatcggt cggtgcccct aagttccttg ggcaaaattg gccggcccca ggtcgagggc
     1621 aacgcactca catttactgt gcaatttcca ccgggcgagg tgggccctgt gctcatcgct
     1681 aacgtcgccg ccccgcaaga ggtgctgatc aacggccagc cggtccccca gaacgaacgg
     1741 cctcacttgg tggagggttc ctcgtggtgt tacgactccg gtatggcgtt actgaccatt
     1801 caggtaggtc tctccggacc ggcggaagtt accgtcaaac ccctaaacgt tcgcacagtg
     1861 gaccgcattc cacggatcgt caacgtcatc cggttctatt tcgatgaggg catggagggc
     1921 tggatggccg cccacgatgt ggctgacctg caggtgaaag ccggccaact cgtcgggcgg
     1981 attgtcgggc cggacccgta cattgttcgt ccgcacatgc gggtaccagg ggccaaaggc
     2041 gacacgatca tcatccgtat aagcacaacg gctggtagca tggcccagct cttttgggcg
     2101 acggaaagct cacccagctt tgacgaagaa aagtcactcc gttttcaagt ccagccaggt
     2161 gagaccgtca acgagtaccg gctgcccctc ggggatcatc catcatggaa cgggcagaca
     2221 gtggtgggac tacggctcga cccagggggt ggcaccacag aagggagctt cgtcgttgac
     2281 gcaatcgagg gtgtgacggc atccggccag cggtgaaacg acaattgctt gctatcccgc
     2341 cgaatgtact attagtcccc acccttgttt ggtactcggg gaatcatttt gtcacgaacg
     2401 actcgtcatt ttggccgtgg caaaggacgt ggccactcgg ccacaagcgc ttgcccacgc
     2461 gaaccgcgga gagaaatctc gtcctaaatt atggattttt gttgtcgggt ggcatttgct
     2521 tgaattacct aacttgccca attatgatcg aaggggtaag tccgatagtg atgtgacgcc
     2581 ttcatgggaa ctttttccgt gagcgccacc gtctaaggac gcaacattgg cgggcgcaac
     2641 atcggccgtt tgacaacgat ggcctgtacc aagtggtgat ggagaggaaa ctcatgcatc
     2701 gttcagctta tcgctgcctt gtggtcgcaa gtctggcgtt gggttttggg ttcttatcgt
     2761 ggggaacatc tgcttgggct cagcagcaag atgacaacca aatggccggg gtgatcgtca
     2821 gccctgatgg tgtcctcacc cggcaaatga tttcggatcc cacgggtcaa atcatgcggg
     2881 agcgggtaat ggcggcccgg cggtcgttgc cacccaacgt ggcccgcttt agcacattgc
     2941 gaaaggtctc cctaccgcgt ttggaaaagg ccattgtagc caaccatggc gtggtgaccg
     3001 atgagatgaa gtacctggcg ggacttctcc gcatccgcta cgtgttcctg tatcctgaaa
     3061 gtggtgacat cgttctggcc ggaccggcgg aagggtgggt cgagacaccc acaggccggg
     3121 tcgtgggaat caacactggc cgccctgtgc ttcagcttca agacttggta acggccttgc
     3181 gtgcttatcc tccggacggt gaaggcgtcg atgtcattgg ctgctccatc gatcccaccc
     3241 aggaaggtct cgcccgcatg caggagttct tgcgtagcct ggggagcacg ttcccaccgg
     3301 gacaagaggc tcaggtcgcc gagtatatgg tcaacggcct gaaaaagagt ttgggtgtcc
     3361 aggaagtcac aatcctggga gtgccgccgg aaacccattt tgcccatgtg ttggttgagg
     3421 ccgactatcg gatgaagctg atcggcatcg gcttggaacg accgcctgtg cgtttggtga
     3481 gcttcgttga acgcgcggct tcgtccgccg tctcgcctaa tcagctattc cgctggtact
     3541 ttgtgcccga ttatcagtgc ctgcgtgtga ccgaggacgg tacggccttg gaattggtcg
     3601 gggaaggcgt caagcttgtg ggtgaagatg aacttgtcac ggccagcggc gagcgaaaag
     3661 cagcctccgc agctagtgcc gcgagtcgtg cgttcacgac cagtttcaca cggcattatc
     3721 cggaactggc cgcaaaggtc cccgtttatg ctgagcttcg caacgtgatt gacatggcga
     3781 tcgttgccgc ctatatcaag cagcaagatt tctacaacca ggccaattgg aaaatggaat
     3841 tcctcggtcg agaagaaaac tttgcggtga agacgtatcc agcccccaag aaggctgaga
     3901 cggccatcac ggcggttatt cgtcctggcc gcgtgagcac acccatcggt ggtggtgtgc
     3961 acattgaagc gcaacaggcc ctcgattacg aaaacctcct caaggacgag aaaggtgagg
     4021 tccaaaaggc caaggccagc gtcaaagtgg atttgccgga aggcgtctgg tggtgggact
     4081 gatcggctgt ttcagtcttt aacgtcttca gttgtgactt gagtctcttt catctcgggc
     4141 gcatagcgct cgagatattt ttttagacgg atcttgcgtc gggcactgtc ggtgctcatg
     4201 taccgaacta gcccaaagat ggggcgatcg gggccccagg ggcgatcata ccggccgaag
     4261 atccagctta tgcccgtata tgaattcgga tcgcttccgt cgaggccgta cttgttgttc
     4321 aggtaaatca tccattccag cgccgtttgc gggtcagggg tccattccag aatctttttc
     4381 ccccaaagca tgcggagata attatgcagg cggccttcct cacgcagctc attctgggcc
     4441 gcgttccaca ggcgatcatg ggtttgcgca ttctccaact gctcaagggt ataagtgata
     4501 ggtcgtctgt ccgaggcgtg cgctgtgaga gttttctttg cccagtcggg aagcgattca
     4561 aaacggtcgt agtcgtcgca acgtaaacaa agattaagcc ccagttcacg ccagacgacg
     4621 agctggtcaa ggaattgagc gatactcggc ggaagattcc accacgtccc ggcgtctgct
     4681 tcctgcgaag gtcgatcgaa cgaccaaccc atttctgtgg cccgtttgag caagtccacg
     4741 atcatttcaa acggtgaaat gaacccgaaa tgcaaatacg gggccaaccc actgacgccg
     4801 ttttcgtcgg ggtgattgcg ccactcgtga taatgctcca gtgcagtttc cagaaactgg
     4861 cgccaacgat tccgtgcggc tgtgggcccg cccttcgtgg ccacaggccc tgccgaatgg
     4921 tcaagcggca gttctttcac accctgacgc gggtttaaca acacctcgtg atcctcccgc
     4981 caagcattcc agaatgggtg tgatggcttt tttgtgcgaa aaggaattct ctcgggaagt
     5041 ggccgcggat caggaagctg gcaaagcgta tcgggcaata gtctctgtac gagagtgcga
     5101 aagccgcggg ctgtgggata atagcgtgag acactccgaa acgggatcaa tcccgatgaa
     5161 tcgaccgctt cgattcgggc acgcgtgcga cttgccaacg actccaaaaa agctctgtac
     5221 tcccgatcag ggtgatcgtc cacgatgata acatcacagg tctggaacaa gctgtaaaga
     5281 tattccacag ctattcgacc aaacttgcga gattgtgccc gatacaaaaa tgggcctcgc
     5341 tcgacgagac tctcatgctc ccgcatccct tccataacga acacggcgtg tcgatcagtc
     5401 aacgtttgat gaaagggcaa cgtttccaaa actgtgagat ccttttcacg gtcggccacc
     5461 cactccaggg cgcgatccac ggcatagttc caggaccagc ggcgattgat gtgcatccaa
     5521 taaagcacca cgttggggga ttgatggggt gtgccttccg caatgacacg gatgcgctct
     5581 tccggaactt tgccgcccca tatcttgccg gaaagaatct gactcgctgc ctccgcaaag
     5641 gcagtagcag gagaagcgat aagccgattc ccacgcattc gttcgttact cggacgtctt
     5701 gttcaaattt cggcgctgcc caagataggg ttgcgcttgg cacacaatgc gcgccacggg
     5761 ccactagagc ttgtccaccg tgggcaccac aaatggttcg cggtattccc gcgtgagcag
     5821 cttgttggcg tcgggccgat cgatgaagca ctcattggcg gcatccacag gcaagagggc
     5881 acccagcgtg agaggctcct tctccaaatc aatgccattc tccttgaggt gctcctcgat
     5941 cgcattcaag gttgccacac agtcttcatg gacctggagt ttttcgagtg ccgccaccaa
     6001 ctccttgtgc ccggcctttt ccccaagcaa gtaggaaata ttggccaggt gcgtcaaggc
     6061 tgttgagata tgcgtttctt caacgggagc attcaacatt tcttgtttgc cggccttcac
     6121 cgcttcgacg aagttgccga agttatccac atttgggcct gtaaggttct cgcggggttg
     6181 tccctgcttg tcatagacca ccacgttggg accaaagctg ctggcaatgt agccctcctc
     6241 gccgacaatg agcaccgccc cgttaagaaa tggcggattg gttttgtggt ttcgcacctc
     6301 ttgaaccagc gtgattccgc ccaggtcatg gacagccacc tgaacgttag gagtttcgcc
     6361 cgcatcgtta aatcccaaac ggccaccgta actgatcacc catttgccca gcgttttgaa
     6421 cgggacgatg atccgagcgg tatccacgca gtggatgcag ttattgccaa tctcgccatt
     6481 accatagtcc cagaaccaat gccagtcgta gtggaacgac ttgcgggtga ccttggccat
     6541 gggagccggt cctgcccaca ggttgtaatc gacgcctggg ggaatggggt aatcgcccgg
     6601 tggcccaatt gggcttcgcg gtcgatacat cacacaccgg atcactttga ctttaccaat
     6661 cttgccggct ttgatagcct cggctgccgc tcgataacga ccttctgacc gcctttgcgt
     6721 cccgccttgg cagagcttgt tatacttttg ggcagcctgg acaatgcgtc gtccctcgct
     6781 gacgttgtgg ctgactggct tttccacata gacatgcttg ccggcttgga tggcccaaat
     6841 cgaggccaag gcgtgccaat gattgggggt ggcaatggag actaaatcca cggccggatc
     6901 ggcgaacaca tcgcggaggt cttgtgcaaa tttcggcttg taaccgaacc tggcggcaaa
     6961 acgctccaca taagtctgcc ccacgtcacg atcggcgtca cacagataga gcacatggca
     7021 atcggggcgc gaagcatacg cactggcgtg atcttgccca cgtccccgaa cgcccaacac
     7081 cgctacctgc aatttatcgt ttggacttgt aggcgccgca gcacctgcag attgggggcc
     7141 ttgctcggcg gccaccacag gaagccccgc ccccaggcca atcccaccga gcgacgctag
     7201 aatggcttcc tgaaggaact ctcttcgtgg ggtaacttgt cgtgccatgg ctagtacctc
     7261 ttttgttaga agatttggta aaaagctcac cagcctcgca gtgtctccca gactggaaaa
     7321 cccacgtcct tttgataccg ttttagccgc cttagtgtcg tcggcactga gctcttgcga
     7381 gccactcact gttctgacgg catttcc
//
LOCUS       DAOPTR010000036         7345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_8378_length_7345_cov_3.820027, whole genome shotgun sequence.
ACCESSION   DAOPTR010000036 DAOPTR010000000
VERSION     DAOPTR010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7345
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8378_length_7345_cov_3.820027"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..131)
                     /locus_tag="PKI05_01315"
     CDS             complement(69..131)
                     /locus_tag="PKI05_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50863.1"
                     /translation="MIRAGANSIREVLAFPFDRA"
     gene            complement(149..1069)
                     /gene="epmA"
                     /locus_tag="PKI05_01320"
     CDS             complement(149..1069)
                     /gene="epmA"
                     /locus_tag="PKI05_01320"
                     /inference="COORDINATES: protein motif:HMM:NF006828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HOA50864.1"
                     /translation="MSWGRAPDDFRPTANWEVLRLRAELLRVIRQFFDERQFLEVETP
                     ILSADTVVDRHLDPFWTADVNRPADITPQTRLYFLQTSPEFGMKRLLAAGSPKAIYQI
                     SRVFRRGECGPLHNPEFTLVEWYRVGDDYHTGMDLLAELCEVLFGRGRPRIYTYGEVF
                     TRFVGIDPYSAAADQLRQRAEALGVAYPASLSLEDRDGWLDLLFTEKVQPHLGQEEPV
                     IICDYPASQAALAIVRKGPPSVAERFELFVNGIELANGYHELRDPEELRQRITTANAQ
                     RTREGKSPLPPTNRLLQAMEAGLPAVSASH"
     gene            complement(1087..1659)
                     /gene="efp"
                     /locus_tag="PKI05_01325"
     CDS             complement(1087..1659)
                     /gene="efp"
                     /locus_tag="PKI05_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008667209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOA50865.1"
                     /translation="MATYNTSEFRKGLKVQLDGEPYLMIECFFVKPGKGQALYKCKLK
                     NLIRNTVIDRTYKSGDTIEAADIEEIDAQFLYRQGDQFVFMDNNTFEQYELTKDQVDE
                     AWKYLKEGMPCTMLLFNGKPISVTPPNHVVLKVEYCEPGARGNTATNVTKPCTVETGA
                     EIIVPAFVEIGDLIKVDTRTGEYIERVKAE"
     gene            1992..3029
                     /locus_tag="PKI05_01330"
     CDS             1992..3029
                     /locus_tag="PKI05_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HOA50866.1"
                     /translation="MWKIHVAQAIRDPAELCSLLDLPPELADEARTAMGNFPLLLPRP
                     MLSRIKPGNPADPVLRQFLPTMPETAAEPGFSTDPLQEQSACPLPGVLAKYEGRILIL
                     ATRACPVHCRYCFRRYFPDTPPTSSPRQTGSRKLPNNPQDVEGSPPTSDGYITSICRY
                     VQSRPDCHEVVLSGGEPLLIDDQQLGTWFSLFSQIAHIQRVRIHTRLPVVIPQRVTPQ
                     LIALFRGAPFRSVVVFHINHPQEIDDAVAEAITRLGHSGAILLCQSVLLKGVNDDPDV
                     LATLYERIAGLRVLPYYLHQLDRVLGAHHFEVPVDRGKKIIEELRKRLPGYLVPRYVR
                     EEPGKDAKVPL"
     gene            3045..5030
                     /locus_tag="PKI05_01335"
     CDS             3045..5030
                     /locus_tag="PKI05_01335"
                     /EC_number="4.2.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjhG/YagF family D-xylonate dehydratase"
                     /protein_id="HOA50867.1"
                     /translation="MASGHPYRDFEEYDALKAADHVPEPVQVFGPGPEGRLPFDDTML
                     RYDPSGHLFGMSQDAGMGWDPAETRRPQFVILSTLGGLRDSEGKPIALGYHTGHFELD
                     EAVAEAAKEIRRLGGMPYAGFCSDPCDGRTQGTPGMFDSLPYRNDAAIVFRRLARSIP
                     RAVGVLGIATCDKGLPAMMMALAGLRDLPVVIVPGGVMLPTTRGEDTAKVQSIGARYA
                     QNEITLEEAQAAACHSCASPGGGCQFLGTAATSQVIAEALGLALPHSALAPSGQPLWR
                     DVARRSAQALVYLAKKGLAGKAILTEAAAHNAMVLHAAFGGSTNLILHLPAVLYETGL
                     PRPSLEDWERVNRQVPRLVDVLPNGPKNHPTLYVYLAGGVPEVMLHLRDLGLLRLDAL
                     TVTGRCLGEILDWWERSPRRHRLRELLRRETGIDPDEVILSPNRAVALGMTGTSCFPR
                     GNLAPGGSVVKSTAIHRRVLDDNGVFDRVLRARVFVREKDAVAAIKGQTSHPVQPGDV
                     VVLIGRGPMGSGMEETYQITSALRYLPWGHEVAVITDARFSGVSTGACIGWVTPEALT
                     GGPIAKVRDGDRIRIVIDTRALQGSINLVGDAEREFSPEEAEHVLKSRTPHPELRPDE
                     HLPEDTRLWAVLQHISGGPWAGCVFDREAILRRLQQT"
     gene            complement(5239..5832)
                     /locus_tag="PKI05_01340"
     CDS             complement(5239..5832)
                     /locus_tag="PKI05_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOA50868.1"
                     /translation="MLHQQIDWELAEREAWALRRQKGIPNNRRLFVLACMDERLPVED
                     ALGIRVGDAHVFRNAGGLVTDDAIRSAMLTTQFFGTKEIIVVNHTECGMMTATGDYLT
                     NLLREKGIDPDAVQVDPALPELKLPPGTFPKWIRTFTDVDEICLRQVELLRSSPLIPA
                     DVEIHGYVWEVESMRLRRPGVRLAEKVNTAKAMGARG"
     gene            complement(6350..6425)
                     /locus_tag="PKI05_01345"
     tRNA            complement(6350..6425)
                     /locus_tag="PKI05_01345"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6387..6389),aa:Pro,seq:ggg)
     gene            6940..>7345
                     /locus_tag="PKI05_01350"
     CDS             6940..>7345
                     /locus_tag="PKI05_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOA50869.1"
                     /translation="MPRFAVILPAAGASSRFRDPHYKKPFAPLAGRAVWLHSLHRFTQ
                     RDDVKQLIVAIAPDDREEFLRRFGADIALLGVEVVDGGRERVDTVSNALTRVREEIDF
                     VCIHDAARPCISDLWIDAVFQKAVETGAAILAI"
BASE COUNT         1421 a         2164 c         2173 g         1587 t
ORIGIN      
        1 gattcgagcg acgctcaccg ccggcccctg tcccgccgaa caggcgagag gaggctgtgg
       61 gatagcctct aagctcggtc aaagggaaat gccaggacct cgcggatcga attggcccct
      121 gcccgaatca tcaccacgcg atcaaaccct aatgcgacgc cgacacggca ggcaatccgg
      181 cttccatagc ctgaagtaat cgattcgttg ggggaagagg gcttttgccc tctctcgtac
      241 gctgggcgtt ggccgtggtg atccgttgac gcagttcctc cggatcgcgc agctcatgat
      301 agccgttggc cagttcgata ccgttgacga acagctcaaa gcgttcggcc acggacgggg
      361 ggcccttccg cacgatggcc agtgccgcct ggcttgccgg atagtcgcag atgatgacgg
      421 gctcctcctg ccccaaatgt ggctggacct tctcagtaaa aagcagatcg agccagccgt
      481 cgcggtcctc cagagagagt gaggctggat aggcaacgcc caaggcttcc gccctttgcc
      541 gtagttggtc ggcagcggcg ctgtaaggat cgatgccaac gaatcgtgtg aagacctcac
      601 cgtaggtata aatcctcgga cgaccccgcc caaagaggac ttcgcacaac tcggcgagaa
      661 ggtccatccc cgtgtggtaa tcgtcgccta cgcgatacca ctccaccagg gtgaactcgg
      721 gattatgaag ggggccacac tctccgcggc gaaaaacgcg gctaatctgg tagatcgcct
      781 tgggactgcc cgccgccaaa agacgcttca tcccgaactc gggagaggtt tgcaaaaagt
      841 agagtcgcgt ttgcggcgta atgtccgccg gtcgattcac gtcggccgtc cagaagggat
      901 cgagatggcg atcgaccacc gtgtcggccg aaaggatggg cgtctcgacc tccaggaatt
      961 gacgctcatc gaaaaactgg cgaattaccc gcagtaactc agcacgaagc cgaagcacct
     1021 cccaattggc cgtgggccga aaatcatcag gtgctcgtcc ccagctcatg aaatggtgtt
     1081 ccgccgctat tccgccttga cgcgctcgat gtactcgccg gtccgcgtat cgaccttgat
     1141 aaggtccccg atttccacga aagcggggac gatgatttca gcaccggtct cgaccgtgca
     1201 tggcttggtg acattggtgg ctgtatttcc gcgggcaccc ggctcgcaat actctacttt
     1261 gaggaccaca tgattgggtg gcgtgacgga aatcggcttg ccattgaata ggagcatcgt
     1321 gcagggcatg ccctccttga ggtacttcca tgcctcgtcc acctgatcct tggtcagctc
     1381 gtactgttca aaggtattgt tgtccatgaa cacgaactga tcgccctgcc ggtaaaggaa
     1441 ttgggcgtcg atttcctcaa tgtcggccgc ctcaatcgtg tcgccgcttt tgtacgtgcg
     1501 gtcaatgacc gtgttgcgga tgagattctt gagcttgcac ttgtaaaggg cctgtccctt
     1561 gcccggcttc acgaagaaac actcaatcat caaatagggc tcgccgtcca attggacctt
     1621 aagtcctttc cgaaattcac tggtattata ggtcgccacg tgtcacaccc tttttcgctt
     1681 gtcaaagttc ttcctacaat acggttatcg gttgtcgaaa cgggatgcgt cggctggtgt
     1741 cacgagcata ccgatccgct ttatttcacc catattattc gtagttcgct aggaatccgt
     1801 ttaccccagc ccgatgatca ccataagcgg caagaaaaca actgaatggc atcgggcagc
     1861 ggatggcgtc cagttctctg aaccgaatct gtcgcatgtc caacacgacg ccaacagcgc
     1921 catcaaccat gccaaatcaa atcgcttgtc aatctgaacc ctccaggacg acgggcggag
     1981 agggaacgcc tttgtggaag atccacgttg cccaggccat tcgtgatccc gcggaactgt
     2041 gttctctcct cgatcttccc ccggagctgg ccgacgaggc acgcacggcg atggggaatt
     2101 tcccactcct cttgccccgg cccatgctgt cccgaatcaa accaggcaac ccagccgatc
     2161 cggtgctcag gcagttcttg cctaccatgc ctgagacggc tgcggaaccg gggttttcca
     2221 ccgatccgct ccaggagcag tcggcatgcc ccctacccgg cgttttggca aaatacgagg
     2281 gtcgaattct tatcctagcg actcgcgctt gtcccgtcca ttgccggtac tgtttccgcc
     2341 gatactttcc cgacactccg ccgacgtcct caccgcgcca aaccggcagt cgcaaactgc
     2401 caaataaccc tcaagatgtg gaaggctcgc ccccgacgtc ggatgggtat atcacgtcca
     2461 tttgccgata tgtccaatcg cggccagatt gtcatgaggt cgtcctcagc gggggtgaac
     2521 cgctcctaat agacgaccag cagttgggga cttggttttc cttgttttcg cagattgccc
     2581 atattcagcg cgtgcggatt cacactcggc tgcctgttgt gatcccccag cgggttacgc
     2641 cgcagctcat cgcccttttc agaggcgccc cgttccggtc ggttgtcgta ttccacatca
     2701 atcatccgca agagatcgac gacgcggtgg cggaggccat tactcggttg ggccattccg
     2761 gcgccatcct cttgtgtcag agtgtcctct taaagggggt aaacgatgac cccgacgtct
     2821 tggcgacgct ctacgagcgg atcgccggcc tgcgggtctt gccgtattac cttcatcagc
     2881 ttgaccgtgt gctgggggcc catcactttg aggtgcccgt ggaccgaggg aagaaaatca
     2941 tcgaagagct acggaaacgc ctgcccgggt atttggtccc gcgatatgtc cgcgaggagc
     3001 cgggcaagga tgctaaggtc cctttgtagt tgttgaggct gtgcatggca tcaggtcatc
     3061 cttaccgcga ttttgaggaa tatgacgcac tgaaggcggc ggatcatgtg ccggaaccgg
     3121 tccaggtctt tgggccaggt cctgaggggc ggctgccgtt tgacgacact atgttgcggt
     3181 atgatcctag tggtcatctt tttgggatga gccaggatgc cgggatgggc tgggaccccg
     3241 ccgagactcg tcggcctcaa tttgtcatcc tgagcacgct gggagggttg cgggattctg
     3301 aaggaaaacc catcgccttg ggctatcaca caggacactt tgagctggat gaggcagtag
     3361 ccgaggccgc gaaagagatt cgtaggctgg gcggaatgcc ctacgcgggg ttttgctctg
     3421 acccatgcga cggccgcaca cagggcacac ccggtatgtt tgacagcttg ccttaccgca
     3481 acgatgcggc catcgtcttc cggcggctgg cgcggtcgat tcccagggca gtcggcgttt
     3541 tgggaattgc cacttgcgat aagggcttgc ccgccatgat gatggccctg gccgggctga
     3601 gggatttgcc cgtggtgatt gtccccggcg gcgtgatgct tcccacgacc cgcggtgagg
     3661 acaccgccaa ggtccagtcg attggtgccc gatatgccca aaatgaaatc acgcttgagg
     3721 aggcccaagc cgcggcttgc cacagctgcg cctcgcccgg gggtgggtgt caattcctgg
     3781 gaaccgcggc cacgtctcag gtgattgccg aggctctggg gcttgccttg ccgcacagcg
     3841 cactggcccc ctcgggtcaa cccttgtggc gcgatgttgc ccgacggtcc gcccaagccc
     3901 tcgtgtatct tgcgaaaaag ggtctcgcag ggaaggcgat tttgaccgag gctgccgccc
     3961 ataacgcgat ggtgctccat gccgcgttcg ggggctcgac gaatttgatt ctgcatcttc
     4021 ccgccgtgct ctacgagacg ggccttcctc gccccagttt ggaagactgg gaacgcgtta
     4081 accggcaggt gccgcgactt gtcgatgtgt tacctaatgg tcccaagaac caccccacct
     4141 tgtacgttta tttggccggc ggggtccccg aagttatgct ccatttgcga gacctgggac
     4201 tgctccgttt ggatgctctt accgtaacgg gccggtgctt gggggaaatc ctcgattggt
     4261 gggaacgatc gccgcggagg catcggctac gtgaactctt gcggcgtgag acaggcattg
     4321 atccggacga ggtcattctt tcgccaaatc gggcggtggc cttggggatg acaggcacat
     4381 catgttttcc gcggggcaat ttggcacccg gcggatccgt ggtcaaaagc acggcgatcc
     4441 accgccgtgt cctcgatgac aacggggtct tcgatcgtgt cctgcgggcg agggtctttg
     4501 tccgcgaaaa agatgccgtc gccgccatca agggtcaaac gtcgcatccg gtgcagcccg
     4561 gcgatgtcgt ggtcttgatt ggtcgcggcc cgatggggtc cggcatggag gaaacctacc
     4621 aaatcacctc cgccctgaga tacctaccgt ggggacatga ggtggccgtc atcaccgatg
     4681 cccgattctc gggcgtgagt accggggcgt gcatcggctg ggtcacgccc gaggccctta
     4741 ccggtggtcc gattgccaag gtccgcgatg gcgaccggat tcggattgtg atcgacaccc
     4801 gggccttgca gggcagtatc aatctggtgg gtgatgccga gcgtgagttc tcgcccgaag
     4861 aggccgaaca cgtcctcaag tcgcggaccc cgcatcccga gttgcgacca gacgagcacc
     4921 tgccggagga tacccgcctt tgggcggtgc tccagcatat cagcggtggc ccctgggccg
     4981 gatgtgtctt cgaccgggag gccatcctcc gccggttgca gcagacgtaa catcagtggg
     5041 agggacgcgg tcccccgcgt cctgccgttt gaaagtgtcg tgccattcgc gaagggcgcg
     5101 ccccccgcgt cagtgcgttt ttccgcggtg gtgtttattg tccacccttt cccgacactt
     5161 gacggtcagg gggatgaact ctcgacccgg cgttacacct gggaagcgag cgatcttgcg
     5221 gcgagcggac cagaccggtt aaccgcgcgc ccccatggcc ttggcggtgt ttaccttttc
     5281 ggccagccgg acgcccggcc gacgcaatcg catcgactcg acttcccaaa cgtaaccatg
     5341 gatctcgaca tcggcgggga tcaagggtga gcttcgcaaa agctcgacct gtcgcaagca
     5401 aatttcatcg acatcggtga aggtccggat ccacttggga aaagtaccgg gcgggagttt
     5461 cagctcgggc aaggccgggt caacctgcac ggcatcagga tcgatccctt tttcccgcag
     5521 gaggttcgtc agatagtcac ccgtcgcggt catcatgccg cattcggtgt gattgaccac
     5581 gatgatttct ttggtgccaa agaattgggt ggtcaacatg gccgagcgga tcgcatcatc
     5641 ggtgaccagg ccaccggcgt tgcgaaagac atgggcatca ccgacgcgga tgccgagggc
     5701 gtcctccacg ggtagccgct cgtccatgca cgctaggaca aagagccgac gattgttggg
     5761 gatacccttc tgccgtcgca atgcccaggc ctcgcgttcg gccaattccc agtcaatctg
     5821 ttggtgaagc atggtttgag gctccttcaa aaatggatag taacccgtaa acaaaccgat
     5881 cctataaatt acttacctga tcgcgtttat taaatgttct gatacgcgat cgacttcacc
     5941 ggtcatttta cgcgatcagg cggaaaagac aagtaataat actacactag tttagttacc
     6001 tttatttgac gaaatccgtt cctggcaggc tcaatgcgag gctgccccaa gggtgatcac
     6061 caccaatgga ctgtctcgtc atggcgggcc atgcggtgtc gcgcccgctt cagactgtag
     6121 gcaacgcgtc gctcaaccca acccattgtg cgcttcgtac tcgaggttca tggcgaagga
     6181 caagcccgat ctctgcacga atcgtgagac cccgctgcaa tcgcacgctg ccaccggaag
     6241 gcaggagtgt tcggcgagcg gctatgcgtt tttcattccc tggcacgggc aaccgtcaag
     6301 acaagaaacg agaaaggccg atcgacagat gcgtcgatcg gccgacgaat cggggcgacc
     6361 ggatttgaac cggtgacctc ttgcacccca agcaagcgcg ctaatccagg ctgcgccacg
     6421 ccccggaatc gttaccgttt ggggattctt ccgagtgttc atcccaccag caccctttaa
     6481 tttaccgcaa aacgagtgcg acggcaacgg ctttgtgcag caaggcgtga ggaaatctga
     6541 aaccccgcct ggagtggctt gttggccgac accgacttag agcctatccc atgatctccc
     6601 ctcgcccgtt tggcgggagg ggggtcgggg gtgagggtgg ctcgagtcgc tgccggtttt
     6661 ctcgatcgat tccgaccacc gcttgccctc accccaaccc tctcccggag ggagaggggg
     6721 actttttggg atagactctt acgccttccc gggccaccgc ccccggtctt cgacagcgca
     6781 ttttccgcgg gttctctagg agaaaggcgg tcccatgtct tatcatgagt caaggaccag
     6841 tcccggcttg ggtctaagag ggttgacggt aatctgagcg actcatgacc gggcatctgg
     6901 ttgatgggtg ttttcgagat ggtttgagga ggcgttttcg tgccgcgatt tgccgtgatt
     6961 ttgcccgccg cgggtgccag ttcgcgtttt cgtgatcctc attacaaaaa gccgtttgcg
     7021 ccacttgcgg ggcgcgctgt gtggctgcat tcgctccatc gctttaccca gcgcgacgat
     7081 gtaaagcaac tcatcgtggc catcgcccca gacgatcggg aagagttcct ccgacgtttt
     7141 ggcgccgaca tcgccttgtt gggtgttgag gtggtggacg gtgggcgcga acgggtggac
     7201 accgtttcta acgccttaac gcgggttcgc gaggagatag attttgtttg cattcatgat
     7261 gccgcccgac cgtgcatcag cgacctttgg attgacgccg tttttcaaaa ggcggtggaa
     7321 accggggcgg cgatccttgc cattc
//
LOCUS       DAOPTR010000037         7273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_8478_length_7273_cov_2.944444, whole genome shotgun sequence.
ACCESSION   DAOPTR010000037 DAOPTR010000000
VERSION     DAOPTR010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7273
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8478_length_7273_cov_2.944444"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PKI05_01355"
     CDS             <1..224
                     /locus_tag="PKI05_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50870.1"
                     /translation="PLDSDEAWVLWMFSTCTTVVCGDAYSLTIAALTWAELAYSELCE
                     GLHNAKLGCRFVVDSRSNAGIVGMQKSGG"
     gene            166..630
                     /locus_tag="PKI05_01360"
     CDS             166..630
                     /locus_tag="PKI05_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50871.1"
                     /translation="MLLTVAATLALSGCKKAADNRPKRVPASVTVTYRGAPVEGATVT
                     LHPTSPDGRAAFGRTNAQGQAELGTFDVNDGAIPGDYNVTVVKMEGGDQAGETPSSEI
                     GAMPANPAGGAPPPPAAPRHLLPAKYADINTSGLRATVNASGDNKITLELTD"
     gene            complement(685..1653)
                     /locus_tag="PKI05_01365"
     CDS             complement(685..1653)
                     /locus_tag="PKI05_01365"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOA50872.1"
                     /translation="MSKDLKKDSRKVRIASPGAADPLDSFRAYCQLIRLPNLFTSAAD
                     ALAGFFVTHPRIAETDLSSLWVIVFSSVAFYAGGVVLNDWVDREVDRQERPHRPIPSG
                     AVSARAAWKLGVGFLLLGLILAALVAWQRQNPWPVIVAALLTTTITFYNFWAKRFFVG
                     PIVMGLCRAENLLLGASLGIGKIAAFWPVTLALGVYVVGISVFARRETERSPRRNLAA
                     GLCLMFAGLGILGVFPLTAQRVVVGQERLWLLLAVLALWMLLRGVWTLADPIPRRVQI
                     TVTQAVMGIIVLDAAAVFVTHGPLGALPVLALVLPTTWAARWIAAT"
     gene            complement(1678..2529)
                     /locus_tag="PKI05_01370"
     CDS             complement(1678..2529)
                     /locus_tag="PKI05_01370"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOA50873.1"
                     /translation="MKLAYSTNAYMRFPFEEAVSRIARLGYTGLELLADVPHAWPMNL
                     LPGDVEHIREILNRHGMAISNINAFMMRGVNDRRQPYWYPSWIEPDPNYRAIRREHTK
                     RALKLAAELGAPGIQTEPGGPLPPGMSRRHAYRIFYDELMPCVEVAEKLGVHLLIEPE
                     PGLLIERFEQFLEFTSMIDSPAVGLNFDIGHAFCVGQSPEVWISTMAPLTRHYHIEDI
                     SAQRVHHHLIPGEGAIDFAAVLKAIRDSHYSGWITVELYPYIDDPDAAGQKALRYLQS
                     VAQSIGN"
     gene            2845..3501
                     /locus_tag="PKI05_01375"
     CDS             2845..3501
                     /locus_tag="PKI05_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOA50874.1"
                     /translation="MTDYSKLYDAIVTGDHRLAVEVTKAALEANVDPSELIAKYMIPA
                     MNEVGNRFACNEYFVPELLIAARAMKASLELITPRLAETGAKRVGRVVIGTVKGDLHD
                     IGKNLVASMLEGAGFEVIDLGVDVPAEKFVQAAQEKPGTIVAMSALLTTTMLNMKSVI
                     EALQQSGLRDKVRIMVGGAPITQQFADQIGADGYGENASAAVQVARKLAAELTQVTAP
                     "
     gene            3630..5264
                     /locus_tag="PKI05_01380"
     CDS             3630..5264
                     /locus_tag="PKI05_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOA50875.1"
                     /translation="MSDHAASESQKPGFRHLLGSGAFPCGVEIVSTRGVPANPEAIPP
                     VVFARELLSLPQVGWISITDSPGGTPMLPPDWIAHFVEDRDRVVIHMTCKDSNRSGLE
                     SFAWRLAAEGFWNVLALTGDYPVTGFGGRPQGVFDLDSVSLIALLKAMNDGLEVLSRS
                     GKPERFPPTQFFIGCAVSPFKRYERELVPQYFKLIRKIATGAQWVITQLGYDMRKYHE
                     VKLFLAARGITNIPVIGNVYLLTRTIAKLFHSGKLPGCVVSDDLMALCDKYGAGPDRG
                     RKFFAELAAKQLAVLKGLGFSAGYLGGLSKPETFSEIMEILGSFAPDDWKIFLREIQF
                     ALPDEFFFFEHDPDTGISSPDRINRQYLASLQKPKRSRYVTLGYRISRWVHALLFTRD
                     RGLWNLAKRLYARWAKKPKPPLPARALYKLEQFSKFMMYGCQDCGDCSLPDCAYVCPK
                     RWCSKCGRNGPCGGSADGRCELQDKECLWAIAYERLKAFGEAESMLEGPPVVYNAELA
                     HTSSWANTYLDRDHHRPREAKPPDGNQTPEGPKANG"
     gene            5353..>7273
                     /locus_tag="PKI05_01385"
     CDS             5353..>7273
                     /locus_tag="PKI05_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50876.1"
                     /translation="MDWSRPAVIALIQYLQASVPVSPGQPPRWDLSGYRVVLPTGWSS
                     RRFLARLADIAAGQKLVLFPPDVITVGRLPETLYPHHRPLADEATCLLAWWAALKELA
                     YADEKLLHRLFPAYQPTMKPTQQLALAQLLASFHREVLGAGYDCQTVLKEALKHDPKF
                     PDQQRWETLCKLEEYYLKALHGCGLWDIDFARREALKNNECRTDREILLVGLVDLPPI
                     VRDMLRQVGNRVTALVLAPDSLHDHFDDLGCLIPEQWQELPVRIPCEKITVCHQPIDQ
                     AHAVIDALAKWGDRFSVEEITIGLPDERLLPLIQEALEQHDLVGRFGAGRPVLQSSVW
                     VLMRSLQQFVSQPTAPAFAALIRHPQMERWLRTRFDQSRDFLTEMDEWIGERLPLLMD
                     AQAMAKEHGPVKEIWEAVWNLLQPEEWTKPQRIDTAGEMIRRVLLDCFGQPDDPDGLQ
                     QNPQTLEICRIILRAIQTWQQVPQVLLDQVPWQGTPLDLLHWVEQQLVHQRLAAEAPG
                     PSIELMGWLDLPWDDAPATIVTMVNEGYIPSAVRSDPLLPPRLREWLGIADNRQRFAR
                     DAYNLSLLVSSREEYQLIAGRQNLDGDTLLPSRFLFSPDDETMVKQVRAYFGLGDQSP
                     RRRPSRWTMAAPNNQH"
BASE COUNT         1525 a         2112 c         2108 g         1528 t
ORIGIN      
        1 cgcccttgga cagtgatgag gcttgggttt tatggatgtt ctcgacgtgt accaccgttg
       61 tctgcggcga tgcctacagt ttgacaatcg ccgcgctgac gtgggcggag ttggcgtatt
      121 cagaactatg tgagggcctt cacaatgcga aattgggttg tcggtttgtt gttgacagtc
      181 gcagcaacgc tggcattgtc gggatgcaaa aaagcggcgg ataaccgtcc caagcgggtt
      241 ccggctagcg ttaccgtaac gtaccgcggg gcaccagtgg aaggcgcaac ggtgacgctc
      301 catccgactt ctccagacgg tagggccgcc tttgggcgaa caaatgcgca gggccaggcc
      361 gagctgggaa ccttcgacgt gaatgatggt gctattccgg gcgattataa cgtgacggtc
      421 gtcaagatgg agggcggtga ccaagctggc gaaaccccgt cgagcgaaat cggcgctatg
      481 cctgcaaacc cggcaggcgg agcgccgccg ccaccagccg ctccccgtca ccttctgccc
      541 gccaagtatg ccgacatcaa cacgtccggc cttcgggcaa cagtaaacgc ttcgggagat
      601 aataaaatca ccctagaact taccgattga ttgtcgccaa tgtcgcgggc caattggcaa
      661 gtcctccggt cttaccccga ccgatcacgt tgcggcaatc caacgcgctg cccaggttgt
      721 gggcaaaacc aaggccaaaa cgggaagtgc tcctagtggg ccatgcgtga cgaacacggc
      781 cgctgcgtcg aggacaataa tccccatgac ggcctgcgtc accgtgatct gcacccggcg
      841 cggtatggga tcggccaagg tccacacccc gcggagcaac atccacaggg ccaggaccgc
      901 caagagaagc cataaccgct cctggcccac gacgaccctt tgggcggtca gaggaaaaac
      961 ccccaggatg cccaggcctg cgaacatcag acaaagtccc gcggccaagt tacgccgggg
     1021 tgaccgttcc gtttcccgcc gggcaaaaac gctaatccct accacgtaca cgcctagtgc
     1081 caaggtcacc ggccagaaag cggcgatttt tccgataccc agcgacgccc ccagtaagag
     1141 gttctccgcc cgacacaggc ccatcacgat tggcccaacg aagaatcgtt tggcccagaa
     1201 gttgtaaaac gtgattgtcg tggtcaaaag cgcggccaca atgacgggcc aagggttctg
     1261 ccgctgccaa gccacaagcg ccgccaaaat cagccccaat aggagaaatc caactcccaa
     1321 cttccaggcc gccctggccg agacggcacc tgaggggatg ggccgatgcg gccgttcctg
     1381 gcgatcgact tcccgatcta cccaatcatt aaggactacg ccacctgcgt aaaacgccac
     1441 actgctgaag acaatgaccc atagcgagga taggtccgtc tcggcgatcc gcggatgggt
     1501 cacaaaaaat ccggccaggg cgtcggctgc tgacgtgaac aggttgggca ggcgaatcaa
     1561 ctggcaatat gcccggaacg agtccaacgg atctgccgct ccgggggaag ctatccggac
     1621 ctttcgagag tctttcttca ggtctttgga catgcgatca tcattttgca aaaaggatca
     1681 atttccgatc gattgtgcca cactctgaag gtatcgcagg gccttttggc cggctgcgtc
     1741 aggatcatcg atataggggt aaagctccac cgtaatccaa ccggaataat gggaatctcg
     1801 aatggccttc agcacggctg cgaaatcaat tgccccttct ccagggataa ggtgatgatg
     1861 gacccgctgg gccgagatgt cttcgatatg atagtgccgc gtcaacgggg ccatggtgga
     1921 aatccacacc tcgggcgatt ggcccacaca aaaggcgtga ccaatatcaa agttaaggcc
     1981 caccgccggt gagtcaatca tgctggtaaa ctcgaggaat tgctcgaatc gctcgatcaa
     2041 aagccctggc tccggctcaa taaggagatg aacgcctagt ttttcggcga cttcaacaca
     2101 gggcatcaat tcatcgtaga aaatacggta ggcatgacgg cgggacatgc cgggcgggag
     2161 tggcccacct ggctcggtct gaatccccgg tgcacccaac tccgccgcca gcttgagtgc
     2221 ccgtttggtg tgttcccgcc gaatcgcccg ataattcgga tcaggttcaa tccaagaggg
     2281 ataccagtac ggctgccgcc gatcgttgac cccacgcatc atgaaggcgt tgatattgga
     2341 aattgccatc ccgtggcggt taagtatctc tcgaatgtgt tccacgtcac cgggaagcag
     2401 attcatcggc caggcgtggg ggacgtcggc caacagttcc agacctgtgt agccgagcct
     2461 ggcgatccgc gagacggctt cctcaaaggg gaaccgcatg taggcatttg tactgtaagc
     2521 aagtttcatc acgattgacg tcctggggat aacgaaagtt gttgttgcgg tgaagactct
     2581 aggaaaccct catagtacag cgctggcttg tatcgggggt aggggcgagg catcccctgc
     2641 ctccaaccgt gttacacaag accactgccg cgatagggtt gtgcgccgat ttgggggagt
     2701 ccgccagggg gctttagctc ttggcaacgg atgccgcacc ctggtaatat aggagggcgc
     2761 gcgggatgag gggaaaaatc ccgttgtggc cgttaacgat gggttttgcc attccttttt
     2821 aagcaagaca tctcggaggg gttcatgact gattactcca aattgtatga cgccattgtg
     2881 acgggtgacc accgtttggc cgtcgaggtt accaaggccg ccttggaggc caatgttgac
     2941 ccgtccgaac tgatcgccaa gtacatgatc ccggcgatga atgaagtggg gaaccgcttc
     3001 gcctgcaacg agtatttcgt gccggagctt ctcattgcgg cgcgggcgat gaaggcctcc
     3061 ctggagttga tcaccccacg cttggccgag acgggagcca aacgtgtcgg acgcgttgtc
     3121 atcggcacgg tcaagggcga tctccacgac attggcaaga accttgtggc ctccatgttg
     3181 gaaggcgctg gcttcgaggt gattgacctt ggtgttgatg tccccgccga gaaattcgtc
     3241 caagcagcgc aggaaaagcc cggcacgatc gtggccatgt cggcccttct caccacaacg
     3301 atgttgaaca tgaagtcggt catcgaggcc ctccaacaaa gcggcttgcg cgacaaggta
     3361 cggatcatgg tcggcggtgc ccccatcacg cagcagtttg ccgatcagat tggcgccgac
     3421 ggctatggcg aaaacgccag cgcggccgtc caagtcgccc gcaagctcgc cgccgagctg
     3481 acgcaagtaa cggccccgtg agggaccttt tacatctgac cggttccgat gtctcatcgc
     3541 atttcccggg cgctatcttg tggaactgga tgcgatgagg gatggcctgt gccaatggcc
     3601 gtttcctcct tagtggggag ttatcttcta tgtccgatca cgcagcgagc gaaagccaga
     3661 agccgggatt tcggcatctc ctgggaagtg gggcatttcc ctgtggtgtg gaaatcgtct
     3721 ccacccgagg tgtccccgcg aatcccgagg ccatcccgcc ggtggtcttc gcccgtgaat
     3781 tactctccct gccgcaagtg ggttggattt ccatcacgga cagccccggg ggcacgccca
     3841 tgctccctcc cgattggatt gcccactttg tggaggatcg ggaccgcgtc gtcattcaca
     3901 tgacgtgtaa agactccaac cggagtggcc tggaatcctt cgcctggcgc ctggccgcag
     3961 agggcttttg gaacgtcttg gcccttacgg gtgattatcc tgtgacgggt ttcggcggac
     4021 gaccgcaggg tgtgttcgat cttgactccg tcagcttgat tgccctcctc aaggccatga
     4081 atgacggcct ggaggtcctt tcccgcagcg gaaaaccaga gcgttttccc ccaacgcagt
     4141 ttttcattgg atgtgctgtt tcgcccttca aacgttacga acgagaactt gtcccccagt
     4201 acttcaaatt gattcgcaag atcgccacgg gggcccaatg ggtcatcacc caactgggct
     4261 acgacatgcg gaaataccac gaggtcaagc tctttctggc agcccgcggc atcaccaaca
     4321 ttccggtcat cggcaatgtt tatctgctca cgcggacgat tgcgaagctc tttcactcgg
     4381 ggaaattgcc cggctgcgtc gtcagcgatg acctgatggc cttgtgcgac aaatacggcg
     4441 ccggccccga tcggggacga aagttttttg cggaattggc agccaaacag ttggcggtcc
     4501 tcaagggact ggggttttct gcgggctatt taggcgggtt gagtaaacca gaaaccttta
     4561 gcgaaatcat ggaaatcttg ggctcctttg cccccgatga ctggaagatt ttcctgcggg
     4621 aaattcagtt tgccttgccc gacgagttct ttttctttga gcatgacccc gacacgggca
     4681 tctcttcgcc cgaccgcatc aatcggcagt atctggcgtc cctgcaaaag ccgaagcgtt
     4741 ctcgctatgt gaccctcggt tatcgcatct cacgatgggt tcatgccctt cttttcacgc
     4801 gggatcgggg cctgtggaat ctcgctaagc gtctctatgc ccgctgggcc aaaaagccga
     4861 aacccccttt gcccgcgcgg gcactctaca agttggagca attctccaaa ttcatgatgt
     4921 acggatgcca ggattgcggg gactgcagtc tgcccgactg cgcttatgtc tgccccaagc
     4981 ggtggtgctc taaatgtggg cggaacggac cctgtggtgg ctcggcagac ggtcggtgcg
     5041 aattgcagga caaggaatgc ctttgggcga ttgcctatga gcgtctgaag gcctttggtg
     5101 aggccgaatc gatgctggaa ggaccgcccg tcgtgtacaa tgcagaattg gcccacacct
     5161 cgtcctgggc caatacgtac ctagaccgtg atcatcaccg tccgcgcgag gcgaaacctc
     5221 cggacggaaa tcagacgcca gaggggccta aagcgaatgg ctagaccgcg ttcatcgaag
     5281 ggatcggatg tcgaagaaca aggtcaatct cccttaagaa cctgcttttc cgggcgtgta
     5341 aagcgggttt ttttggattg gtctcgcccc gccgtgatag ccctgatcca gtatctccag
     5401 gccagtgtgc ctgtgtcgcc gggtcaacct ccacggtggg acctctccgg ctaccgcgtc
     5461 gtcctgccca ccggttggtc gtcgcgtcgc ttcctcgccc gtttggcaga tatcgccgcc
     5521 ggtcaaaaac ttgtgctttt cccgcccgat gtgatcaccg tggggcggct tcccgagacg
     5581 ttatatcccc atcatcgacc tctggccgac gaggccacct gtttgctggc gtggtgggcg
     5641 gcccttaagg agttggccta cgctgacgaa aagctcctcc atcgcctctt tccggcctat
     5701 cagcctacca tgaaacccac ccaacagctc gccctggccc aactgttggc cagctttcac
     5761 cgggaagtgc ttggggccgg ttatgattgt cagacagttc tgaaggaggc cctcaagcat
     5821 gatcctaagt ttcccgacca acaacgttgg gaaacccttt gcaagctgga ggaatattat
     5881 ctcaaggcac ttcacggctg tgggctttgg gatattgatt tcgcgcgtcg ggaggccctc
     5941 aaaaacaatg aatgccgcac ggacagagag attcttctcg tcgggctggt cgatttgccg
     6001 cccatcgtcc gcgacatgct gcggcaagtg gggaatcgcg tcacggcttt ggtcctggcc
     6061 ccggactcac tgcacgatca ttttgacgat ctgggctgct tgatccccga acagtggcaa
     6121 gagctgccgg tccgtatccc ctgcgagaag atcaccgtat gccatcagcc cattgaccag
     6181 gcccatgccg tgatcgacgc cttagcgaaa tggggcgatc gcttctcggt cgaggagatc
     6241 accattggtt tgccggacga acgtttgctt ccgctcatcc aagaggcttt ggagcaacat
     6301 gatctggtcg gtcggtttgg tgccggtcgt cctgtgctgc aaagctccgt atgggtcctg
     6361 atgcgaagtc ttcagcagtt tgtcagccaa ccgacggccc cggcctttgc cgccctgatt
     6421 cgtcatcccc agatggagcg gtggttacgg acgcgtttcg atcagtcacg agacttcctc
     6481 accgaaatgg acgagtggat cggcgaacgg cttcccctat tgatggatgc ccaggccatg
     6541 gccaaggaac atggcccggt caaggaaatc tgggaggccg tctggaatct cttgcaacca
     6601 gaggaatgga caaagcccca acgaatcgac acagccggag aaatgatccg ccgcgtgttg
     6661 cttgattgct ttggacagcc cgacgatccc gacgggttgc agcagaatcc ccagaccctt
     6721 gaaatctgcc ggatcatttt gagagcaatt caaacatggc agcaggttcc gcaagtgctt
     6781 cttgatcagg tgccgtggca agggacaccg cttgacctac tgcactgggt cgaacagcag
     6841 ttagttcatc agcggttggc tgcggaagcg ccggggcctt ccatcgagtt gatgggttgg
     6901 ttggacctgc cctgggacga tgccccggcc acgatcgtga cgatggtcaa cgaagggtac
     6961 attccgtcgg cggtgaggag tgatcccctc ttgccgccac gtctgcggga atggctgggg
     7021 atcgccgaca atcgccagcg atttgcccgc gatgcttaca acctaagctt actcgtctcg
     7081 tcccgggagg aatatcaatt gatcgccggc cggcaaaacc tggatggcga taccctcttg
     7141 cccagccggt tcctgttttc cccagatgat gaaacgatgg tgaaacaagt tcgggcgtac
     7201 tttggtttgg gcgatcaatc gccgcgacgc cgtccatccc gctggacaat ggccgccccg
     7261 aacaaccagc act
//
LOCUS       DAOPTR010000038         7221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_8559_length_7221_cov_3.493860, whole genome shotgun sequence.
ACCESSION   DAOPTR010000038 DAOPTR010000000
VERSION     DAOPTR010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7221
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8559_length_7221_cov_3.493860"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PKI05_01390"
     CDS             <1..702
                     /locus_tag="PKI05_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOA50877.1"
                     /translation="FDDFDLNRFFQERYRDDFLGGRPSGTGFEELFAQFQRAAGRARP
                     ETGRAAYRGQDLHSEVTIPFNVAVEGGKVDITVNRPGVGRETLAVMIPAGIEDGKKIR
                     LKGKGAPGPAGGEPGDLYLTVHVAPHRWFERKGNDLYVKVPVTIPEAIFGAKVEIPTP
                     KGRIALRIPPGAASGKKLRVPGYGVHPRDGTPGDLYAEVQIVPPPQVPESLKSSIEQL
                     AREYPRDVRADLHWP"
     gene            738..1589
                     /gene="yidD"
                     /locus_tag="PKI05_01395"
     CDS             738..1589
                     /gene="yidD"
                     /locus_tag="PKI05_01395"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOA50878.1"
                     /translation="MSIKRDRVLMGEFSFGREYRIRRQKDIDRVFARRCSVADDVIII
                     YGCENGQGLPRLAIMASRKLGKSVVRNRWKRLIREVFRLNKHRIPRGVDYVVIPKPGA
                     TPDFRTLFRTFPHLAKQLKRRLDRSAKREESGQATSPGVSSSFQEKAEGRESSRETHP
                     SRGLPQTDVDEATHYVVEPTANGDDLGDDPAEVGFRGLRKITRPIWRGLQLIDAAIGE
                     VLILPVYVYRWTISPLLGHCCRFEPSCSQYFIQAVRKYGAIMGSLRGLARILRCHPWH
                     PGGYDPP"
     gene            2037..3239
                     /locus_tag="PKI05_01400"
     CDS             2037..3239
                     /locus_tag="PKI05_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA50879.1"
                     /translation="MSSKDYSPFTPGQPVPVEFFVGRSKEIDHIISAVRASTAGRLQV
                     LFLTGERGIGKSSLARLVRIIADRKYGVLGLHSFLGGVTSLDETVRRVFDRLLKESVG
                     KGWWAKIKEFFGDHIRQVDLFGVTVEFGAAEKELRRMVHDFAPALRNLLQKLRPEKKG
                     ILVVLDDINGLAASDQFANWLKSLVDEIATSSDPLPLCLMLVGLEDRRQSLVSLQPSL
                     ARVFDVVELRTWSDAETRDFFVNAFSKVGIHVEEEALKILVRFTGGFPVMAHEIGDAT
                     FNEDTDNRITREDALQGVVRAADIVGRKHVEPQVFTAIRSPRYRAILRKTASKPFEVE
                     FRRRDLWPLLKDDERKVFDNFLARMKKLGVVQANPEGGPGAYRFTNTLHYLYFWLEAA
                     RAKQSKEL"
     gene            3580..4689
                     /locus_tag="PKI05_01405"
     CDS             3580..4689
                     /locus_tag="PKI05_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOA50880.1"
                     /translation="MSAEARDSVPFFQRRERLRRRLSKDGLAGMLITKTENVRYLTGF
                     GGDDSYLLLWKDHEILISDSRFTEQIRQECPGLEAHIRRTGVSMHQAAANLINRAKLT
                     SVAVEASLPMATYKKLKAALKQTELVEAEDWIEQLRAVKDKTEIARIRKAVQYAERGF
                     AAVRATLRPEHSEKEIADLLEYQMRTLGARCSSFPTIVAVGERAALPHAIPSDRKVSE
                     NVFILIDWGADEGWYKSDLTRVLITGRISPKFAKVYQTVLRAQQAAIAAVRPGVSAQE
                     VDAAARQVIADAGFGRLFGHGLGHGVGLEIHELPRLGPNSSITLKPGMVITIEPGIYI
                     PDWGGVRLEDDVLVTRDGCEVLTRDPKALEEVVVF"
     gene            4729..5298
                     /gene="accB"
                     /locus_tag="PKI05_01410"
     CDS             4729..5298
                     /gene="accB"
                     /locus_tag="PKI05_01410"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008695239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOA50881.1"
                     /translation="MASDVPDNEGRDFPEVPDVFDLARVRELVELMRAHELSEIDLRE
                     GKRRIRLRRGPESVPAATGPAVPFPIFMPGSAPVPPPTPAGPVTPPPVREPEATSEAP
                     KQKEEENIVFIRSPMVGTFYAAPDPDSPPYVKVGDMVGPDTVVGIVEAMKVFNEIPAE
                     VSGQIVAILVENGEPVEYGQPLFKVDTKA"
     gene            5356..6708
                     /gene="accC"
                     /locus_tag="PKI05_01415"
     CDS             5356..6708
                     /gene="accC"
                     /locus_tag="PKI05_01415"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOA50882.1"
                     /translation="MFKRILIANRGEIALRVIRACREMGIETVVVFSEADRGSAYLEL
                     ADEAYCIGPPKASESYLRYDRVISAAEIGNAQAIHPGYGFLSENAHFAEVCRNCQIEF
                     IGPPHEAMALLGDKNAARALAKKVGVPIVPGSEGTVETETEAIRVAREIGYPVLIKAA
                     AGGGGKGMRIAVNETALKTAFQQAQQEAEAAFGNGAVYLEKYIEHPRHVEVQAIGDQY
                     GNVVHLWERDCTIQRRHQKLIEESPAPQLPQEVRQAICEAAVKLLKAANYYNAGTVEF
                     LVDRQFNFYFIEVNARIQVEHPVTEMVTGLDLVKLQIKVAAGEPLPFKQSDVEHRGVA
                     IECRINAEDPERQFRPSPGRIERMFVPGGPGVRFDSHVYPGYVVSPHYDSMIGKLIVH
                     RPTRAEAIAAMKKALEELRIEGIKTTIGLIQAILSHPTFVDARADTTFVERTWTFNQA
                     "
BASE COUNT         1580 a         1835 c         2187 g         1619 t
ORIGIN      
        1 ttcgacgact tcgacttgaa caggtttttc caagagcggt atcgagacga tttcctcggc
       61 ggtcggccct ccggtaccgg ttttgaggag ttgttcgctc aattccaacg agccgcgggc
      121 agggcccggc cagagacagg gcgtgctgcc taccgcggcc aagatttaca cagcgaagtg
      181 acgattcctt tcaacgtggc tgtggagggt ggcaaggttg atatcacggt taaccggccg
      241 ggagttggcc gggagacttt ggccgtcatg attcctgcgg ggatcgaaga cggaaagaaa
      301 atccggctga aagggaaagg ggcccctggt ccggcgggtg gagagcctgg cgatctttac
      361 ctcacggtcc atgttgcccc tcatcgatgg ttcgaacgga aagggaacga cctctacgtg
      421 aaggttcccg ttaccattcc ggaagccatt tttggggcga aggtcgaaat cccgacaccc
      481 aaaggccgca ttgctctgcg gattcctccg ggagcagcca gcgggaagaa actccgcgtc
      541 ccggggtatg gcgttcatcc gcgagatggc acgcccggtg acctctacgc ggaagtgcaa
      601 atcgtcccgc cgcctcaggt cccggaaagt ttgaaatcgt caatcgaaca attagcccgc
      661 gagtatcccc gcgacgtgcg ggcggacttg cattggccat gattggggct gtccgataac
      721 gacgaacggg ccgttttgtg agtatcaaac gggaccgggt cctaatgggt gagttttctt
      781 ttggtcgtga gtatcgaatc cgcaggcaga aagacattga ccgcgttttc gcgcggcgat
      841 gctcggttgc cgacgacgtg atcatcatct atggctgcga aaatggccaa ggcctgcccc
      901 gactagccat catggcttct cgtaaattgg gcaagagcgt ggtcaggaat cgctggaaac
      961 gcttgattcg agaggttttc cgcttgaata agcacagaat cccgcgcggt gtggattacg
     1021 ttgtcattcc caagccgggg gctacaccgg atttccgcac gctatttcga acctttccgc
     1081 atttggcaaa acagctcaag cggcgactag atcggtcggc aaaaagggag gaaagcggcc
     1141 aagcgacaag cccgggggtg tcttcctctt ttcaggagaa ggccgaaggc cgcgagtctt
     1201 cccgggaaac gcatccatct cgcggacttc ctcaaacgga cgtggacgag gcaactcatt
     1261 acgtagttga gccgacggcc aacggggacg acctggggga tgatccggcc gaggtggggt
     1321 tcaggggcct gcgaaagatt acccgcccga tttggcgggg tcttcagttg atcgatgccg
     1381 ccatcggcga ggttttgatt ctgccggttt atgtttaccg ctggacgatt agcccgctgt
     1441 tgggccactg ctgccgtttc gagccaagct gttcacagta ttttattcag gccgtgcgca
     1501 aatatggggc catcatggga agtttgaggg gcttggcgcg gattcttcgc tgtcaccctt
     1561 ggcaccctgg gggttatgat cctccgtaag atgtgagccc gaaccaggtg gtacttttta
     1621 gcgaaagtcc aaaagcggca ggatggcctg caaggcgatg acacctccct gtataccgta
     1681 taccgttccg gccccccggc tgcgttttac gaattagagc ctgtcctgta atctcccctc
     1741 acccgttcgg cgagagaggg gtgggggtga aagtggctcg aatcgctgct ggtgttctca
     1801 atcgattctg acgctggctt gccctcaccc caaccctctc ctggagggag agggggactt
     1861 attgggatag gcacttagtt gcagacaacg gcatcccttc cactaggcgt gtgcttcggg
     1921 aaggtttccc atgtctcgtg aacagttact tgattgcgtt tccccaacgg gacaatacga
     1981 ttatactgcc cgatagtgag gagtggtgca tggttccgtg aaaaatggag cgcaaaatgt
     2041 catctaagga ctacagtccc ttcacacccg gccagcccgt accagtcgag ttttttgttg
     2101 ggcgttcgaa ggaaatcgac cacattatct cggctgtgag ggcttctacg gcaggaaggc
     2161 ttcaggtgct cttcctaacg ggagaaagag ggataggaaa gagctccctt gcgagattgg
     2221 tgcggattat tgcagaccgt aaatatggag ttctcggact gcacagcttt cttggcggtg
     2281 tgacgtccct tgatgagacg gtgcggcggg ttttcgaccg tcttctcaag gagagtgtgg
     2341 gcaagggttg gtgggcaaaa ataaaagaat tctttggcga tcacatccgg caggtggacc
     2401 tattcggtgt caccgttgag ttcggtgccg cagagaagga actccgtcgc atggtccacg
     2461 attttgcacc agcattgcgg aaccttctcc agaaattgcg tccagagaaa aagggcatcc
     2521 tggtggtgtt ggacgatatc aatggtctcg ctgcctcgga tcagtttgcc aattggctga
     2581 agagcctcgt ggacgaaatt gccacttcgt cggatccctt gcctctgtgt ctgatgctcg
     2641 tagggttgga ggatcgccga cagtcacttg tgtcgcttca gccttccttg gcgagggtgt
     2701 tcgacgtggt ggaactccgg acgtggtcag acgcggaaac ccgggacttt tttgttaatg
     2761 ccttctcgaa agtgggcatt cacgtagaag aagaggccct caaaatctta gtgcgtttta
     2821 cgggggggtt tcccgttatg gcgcatgaaa ttggggacgc gacctttaat gaagacacag
     2881 acaatcggat tacgcgagag gatgcacttc aaggggttgt ccgagcagca gacatcgtgg
     2941 ggcgcaaaca cgtggaaccg caggttttca cggcgattcg tagcccgcga tatcgagcaa
     3001 ttctcagaaa gaccgcatca aagccattcg aggtagaatt tcgcaggcgt gacctttggc
     3061 cacttcttaa agacgacgaa aggaaggtat tcgacaactt tctcgcccgc atgaagaaac
     3121 ttggggtcgt tcaagcgaac ccagaaggtg gtcccggtgc atatcgtttt accaatacct
     3181 tgcactacct ctatttctgg ttggaagccg cgcgggcgaa gcaatctaaa gaattatgac
     3241 ccgcgcccgc aggtcagtcg aaagagtgtc ttcttgcaaa gtatcactat cgccgcgcaa
     3301 aagaacgttt ttcaaaggac ctatagcgat gttcgccctc aggcaatcgt taggcaaagg
     3361 cttctggggc atacagcttg gatgaccgta ccggccatgt ttgccggtca atccggtcag
     3421 gtgaaaaggc cgtagggggt tgccaatgct ctgtgcgcta cctggaaacg gaagcgcgtt
     3481 ttgccagcat ttctgttggc taaagggaca tcttgccgcg atggtcggaa ttccttaatc
     3541 tggcgcgaat acgtttgatt gcagattagg agctatacga tgtccgcaga agcccgggac
     3601 tctgttccat tttttcaacg tcgcgaacga cttcgccgcc gactcagtaa ggacggcctg
     3661 gcgggaatgc ttattaccaa aaccgagaat gtccgttacc tcactggctt tggaggtgac
     3721 gatagctatt tgctcctttg gaaagaccac gaaatcctta tcagtgattc gcgcttcacg
     3781 gagcaaattc gtcaggagtg tcctggtctg gaggcccata tccgtcgcac gggcgtttca
     3841 atgcaccagg ccgccgcaaa cctaatcaat cgggcgaaat tgacctccgt ggccgtcgag
     3901 gcgtccctcc ccatggcgac ttacaaaaag ctgaaggcgg ccctcaaaca gacggagctt
     3961 gttgaggcag aggattggat cgagcaattg cgggcggtta aggataagac ggaaattgcc
     4021 cgcattcgca aggcggtcca gtacgcagaa cgggggtttg cagcggtccg ggccacgctc
     4081 cgcccggaac attcggagaa agaaatcgcc gacctattag agtatcaaat gcgcaccttg
     4141 ggagcacgtt gttcttcctt tccaaccatc gtggcggtcg gagaaagggc cgcgctaccg
     4201 catgcaattc cttcggatag gaaagttagt gaaaatgtct tcatcctgat agactggggg
     4261 gctgacgaag gttggtacaa gagcgacttg acccgtgtcc ttattactgg tagaatctcc
     4321 cccaagtttg ctaaggtcta tcaaacggtt ttgcgtgctc agcaagcggc gattgctgct
     4381 gtgcggccgg gtgtgtccgc ccaggaggtc gatgcggcgg cccgccaagt cattgcagat
     4441 gccggtttcg ggcggctttt tgggcatgga ttggggcacg gggtgggtct ggaaatccat
     4501 gaattgccgc ggcttgggcc aaacagctca attaccctca aacccggcat ggtgataacc
     4561 attgagccag ggatttacat ccccgattgg ggtggggtgc ggctggagga tgacgttctt
     4621 gtgacgcggg atggttgtga ggtcctaacc cgagacccca aggctttgga agaggtcgtc
     4681 gtcttttaat tgacacaatt gccagtagaa agaggtttga cttcgcgcat ggcctcggac
     4741 gttcctgaca acgaaggtcg agacttccca gaggtgccag acgtcttcga tcttgctcgc
     4801 gttcgtgagt tggtcgagtt gatgcgggca catgagttaa gtgaaatcga tctgagagag
     4861 ggcaaacgcc ggattcgttt gcggcgcgga cctgagagtg tcccggcagc gaccgggccg
     4921 gcagttccct ttcccatctt catgcctggc agcgcgccag tgcccccgcc aacaccggcc
     4981 ggaccggtta cacccccgcc cgttcgggag ccggaggcga cttctgaggc gcccaaacag
     5041 aaggaagaag agaacattgt cttcattcgc agtcccatgg tggggacgtt ctatgcagcg
     5101 ccagatcccg attcgccccc ttatgtcaaa gtcggtgata tggttgggcc ggatacggtg
     5161 gtcgggatcg tcgaggccat gaaggtgttc aatgagatcc cggccgaagt ttccggacag
     5221 atcgtcgcca tcttggtaga aaacggggaa ccggtcgaat acggtcagcc attgttcaag
     5281 gtagatacca aagcctgagt cggctcgatg tgacaaaata gtcaaatggg ccacctcata
     5341 tcccggttgt agcgcatgtt taagaggatt cttatcgcca atcgaggcga aattgctttg
     5401 cgagtgatcc gggcctgtcg tgagatgggc atcgagaccg ttgtcgtttt cagtgaagcc
     5461 gaccgcgggt cagcttatct tgaattggcc gacgaggcgt attgcattgg tccccccaag
     5521 gcatctgaga gttacctccg ctacgatcgg gtgatcagtg ccgcggaaat cggaaacgcc
     5581 caagcgattc atcccggcta cggttttctc tcagaaaatg cccattttgc cgaagtttgc
     5641 cgcaattgtc agatcgagtt tattggtccc ccgcatgagg cgatggcgct acttggggac
     5701 aagaacgccg cccgggcgtt ggccaagaag gtaggtgtcc caattgttcc ggggagtgag
     5761 ggcactgtcg agacggaaac cgaggcgatt cgggtggcgc gagagattgg ctatcccgtc
     5821 ctcattaagg cagccgccgg tggcggggga aaagggatgc ggattgccgt taacgagacc
     5881 gccttgaaga cggcctttca gcaggcgcaa caagaggccg aggctgcgtt cggcaacggc
     5941 gctgtctatc tggagaaata tattgagcat ccgcgccacg tcgaggtcca ggccatcgga
     6001 gaccagtacg gcaacgtagt acacttgtgg gaacgcgact gtacgattca gcgtcggcat
     6061 cagaaattga tcgaggaaag tccggcgccg cagctccccc aagaggttcg acaagcgatc
     6121 tgtgaggcag cggtaaaact cttaaaggcc gccaattact acaatgcggg aaccgtggag
     6181 ttcttggtgg atcggcagtt caatttctac tttattgagg tcaatgctcg aatccaggtc
     6241 gagcatcccg tcacggaaat ggtgacgggc ctagatttgg tcaagttgca aattaaagtt
     6301 gcggccgggg aaccgttacc attcaaacag tcggatgttg aacatcgtgg ggtggccatt
     6361 gagtgtcgga tcaatgccga ggacccagaa cggcaattcc gaccgtcacc gggtcggatc
     6421 gagcggatgt ttgtccccgg ggggccggga gtccgtttcg attcccatgt gtatcccgga
     6481 tatgttgttt cgccccatta tgattcgatg attgggaaat tgatcgtcca tcggccgacg
     6541 cgggcagagg ccatcgccgc gatgaaaaag gccctggagg aattgcgaat tgaaggaatc
     6601 aaaacaacga tcggcctgat ccaggcgatt ctttcgcatc cgacatttgt ggatgcgcgg
     6661 gccgatacga cttttgtgga acggacgtgg acatttaacc aagcttaagg gctcgtcctt
     6721 ttcggacgtt catgtgagcg ggttctcaag gcgggaaaat ctcgggatcg ccttctgcgg
     6781 ttccggaacc atagggggtt gcggggcgta ataagagtct atcgcaaaaa gtcgccctct
     6841 ccctctggga gagggttggg atgagggcaa gcgaccgtgg gaatcgattg agaaaaccgg
     6901 caatgattcg agccaccctc acccccgacc cctttcccgc caaacgggcg aggggagatt
     6961 atgggatagg ctctacgatg ttaatctggt gaagcttgta atgtggtcgc atatagcaca
     7021 ataagcacaa gagaggatgg ttgaacaatg agtgacgcac ttgcaagtcc ggcgaaggtt
     7081 gaaagcccta tcaagcgggt tgcaatcttg ttttctggtg gtccggctcc ggcggccaat
     7141 gccgtgatcg ccagcgcggc catcgcgttt tcccgcgttg gcgtggaggt cctcggcatc
     7201 cgccacggct acacacattt g
//
LOCUS       DAOPTR010000039         7212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_8573_length_7212_cov_3.680173, whole genome shotgun sequence.
ACCESSION   DAOPTR010000039 DAOPTR010000000
VERSION     DAOPTR010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7212
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8573_length_7212_cov_3.680173"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /locus_tag="PKI05_01420"
     CDS             <1..1106
                     /locus_tag="PKI05_01420"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOA50883.1"
                     /translation="EVAQRYSELAETYRNYARHGLKFLRTVMWDREYGGFYWGVDQNG
                     QVKPSYGQEKHLYGISFGLYAAANVYRATGDADALQLAQDTFAWLEKHAWDRKHGGYW
                     EAFARNGTPLISPPPPGSDGRANPQRDLLGTPYGFKSMNSHIHILEALTELHRAWPSE
                     EVTNRLREVFFIVRDKIAVEPGCLNLYFTPDWRAVPDHDSFGHDVETAYLLLEAAERL
                     HLANDPATIHRAKSLVDHALDYGWDNRLGGFYDKGTAFGQPYGLEKIWWTQAEGLNAL
                     LLMHELFGDDDPRYWPQFVKQWQFIKQYQIDHNTGEWFDTVSAEGQPQIKDLGHIWKA
                     AYHNGRALMECGTRLRRLAGMNGLTPHEKSGKR"
     gene            1419..3317
                     /gene="dnaG"
                     /locus_tag="PKI05_01425"
     CDS             1419..3317
                     /gene="dnaG"
                     /locus_tag="PKI05_01425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOA50884.1"
                     /translation="MELPFAGAVIEGLPTWEPISTMPLTPYSDVKEQIRQAIDIVDLV
                     ERYIPLRRQGRGFVGLCPWHDDHRPSLQVNPQRQSFRCFVCDIGGDIFTFMMKIENVE
                     FPEAMAMLAEMAGIPLPRGIGRGSAVSQQKQDLYRVMAWAEQQYHQCLLEAPEAAPAR
                     EYLASRQISPASIAQFRLGFSPLQPDWLLRRARRDQIDPKDLVTTGILVGTPGRDLID
                     RFRGRVLFSIRDSQGRTVGFGGRILPHMAEQVPAKYVNSPETPLFSKSRLLYGLDVAR
                     DAFRRTGTALVMEGYTDVIMAHQCGFHNAVAVLGTALTDAHLQVLQRFVDRVILVLDG
                     DEAGRRRAAQVLELFVARQIDLRVLTLPDDLDPCDFLLKYGAPGFQDALDTRTVDALE
                     HAFAAATHGVDLERDIDGVTRAVDRLLMVMAKAPAKLGQHTAQQQAREWKILSRLSVM
                     TRIDESVLRKRLEELRRGGRSRTAAVATPAPKTTLDGWHRTFLEFLVTFPDAGEKLRA
                     AFQPEQIEIPLVRRIFEIALDLLANGKPADFDHLMLAVDDPLVHNILVDVDQTASEKR
                     LAEQNPEEILRGLLRAHEERVARARGSQLTHVLRQGGLDPEQQIDLLKRIVEQERNRQ
                     KWSEPMDG"
     gene            3428..5086
                     /locus_tag="PKI05_01430"
     CDS             3428..5086
                     /locus_tag="PKI05_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOA50885.1"
                     /translation="MKHGDKELRRLIAQGRAQGYLTYDQVIEYLPDQGSRPEKLDRLI
                     TALEEAGIEMVNRPPRSETSANAAGEPQDEDPLLKDPLLRDLLPGEKAKWNSDPIRLY
                     LSQMADIPLLTRQQEVALAKKIEVTRKRFRRTVMGCHLVLKQAVQTLEAVQRGDLPFE
                     RTLKMSLTDQLTKNQILGRMPHNLRTLRALMAENEKDFRRMINRRTPEEVKAKARRRF
                     LARWARMLILVEELGLRTRRVQSMMQQMRDILKRMETIRARLQELKTQPGELGERERL
                     RRELMALMLCTQETPRSLRIRCEKMETQFRDYSEAKRELCNSNLRLVVSVAKRYRNRG
                     LSFLDLIQEGNTGLMRAVDKFEYRRGFKFSTYATWWIRQAITRALAEHSRTIRIPVHI
                     IDQIGKLKNTSRRLMHELGREPTPEEMAQATALPAEDASRLLEMGRQPTSLDRPIGED
                     EDNCFSELLADHTLDKPENIAHRDLLREKIDNLLKTLTYREREIIRLRYGLGDGYNYT
                     LEDVGRIFKVTRERVRQIEAKAMEKLQHPVRAQQLAPFVDGLVG"
     gene            5174..5911
                     /locus_tag="PKI05_01435"
     CDS             5174..5911
                     /locus_tag="PKI05_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50886.1"
                     /translation="MSSQALTIPEILRRLHGLHQQMDQLRGRLMKGPRLAQAHQEQIR
                     QAENNLDQAKKAYNDTRLASHRKQLEYEQKLADIQRRKGRLLECDDNREYQLLKDQIA
                     ADEMAASVLADEAIELMTRVEEMAVKVQEAEAALAAAKDAAAKWQAEFEKQEPVIRQE
                     IAQVEQELKHLETLLTGDVRELYQRALRNKGSSALAPLRGEFCGGCNQHVPLQMRSQL
                     LLGNAIWCRSCGRLLYLPENENPHSRS"
     gene            complement(5908..>7212)
                     /locus_tag="PKI05_01440"
     CDS             complement(5908..>7212)
                     /locus_tag="PKI05_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOA50887.1"
                     /translation="IDEAHTLESVASEQFGTDVSYTQILFNLRKLYNERTQRGLFVHY
                     RSPEGQKAVMDCLYRCDEFFAQVFDWYEKQKPGNGRVRQRDVFRNTLTEGLQKLASIV
                     HKEAARIEKEEERQDLVAVRDRLQSLATQVDQWIRQSHEGYVYWVEVQEARQVPRIIL
                     GAAPIDVGPILREHLFQEIPRVILTSATLAVAESGFEYFKSRIGLTRADTLQVGSPFN
                     YQEQAELIIVRDMPDPSLDSSQFEKATVVALKRYIARTERRAFVLFTSYDFMRRIAAA
                     LQSWFAKENYTLFSQAEGLPRSQMLERFKTTPRAVLFGTDSFWQGVDVPGEALQNVII
                     TKLPFAVPDRPLVEARLEAIKAAGRSPFQEYQLPEAVIKLRQGFGRLIRSRADRGIVV
                     ILDPRVYQKSYGRVFLRSLPPCRIKEEQLFDSGRLPACDPSS"
BASE COUNT         1659 a         1939 c         2083 g         1531 t
ORIGIN      
        1 ccgaagtggc gcagcgctat tcggaattgg ccgagacata ccgcaactat gcccgacatg
       61 gattgaaatt tctccgcacc gtcatgtggg atcgggaata cggcggtttt tattggggag
      121 ttgatcaaaa tggacaagtg aagccgagtt atggccaaga aaagcatctt tatgggatat
      181 ccttcggatt gtatgcggca gccaacgttt atcgtgccac gggcgatgcc gacgccttgc
      241 agttagccca ggatacgttt gcctggctgg aaaagcatgc gtgggataga aaacacggcg
      301 gctattggga agcctttgca cggaatggca cgccgctgat ttcaccaccc ccaccgggaa
      361 gcgacggccg ggctaatcct cagcgcgatc tgctaggtac tccctacggc ttcaaatcga
      421 tgaacagcca tattcacatt ttggaggccc tcaccgaact gcaccgcgct tggccaagcg
      481 aggaagttac caaccggttg cgagaagtat tctttattgt ccgcgacaaa atcgccgtgg
      541 agccaggctg cctcaatctc tatttcacac ccgattggcg agccgtcccg gaccacgatt
      601 cgtttgggca tgatgtggaa acggcctatc ttttgctgga ggccgccgaa cgcctccatc
      661 tggcgaacga tccggcgacg attcaccggg caaaaagcct cgtcgatcac gcactggact
      721 acggttggga caatcgcctc ggcgggtttt acgacaaggg gacggccttt ggtcagcctt
      781 acggattgga aaaaatttgg tggacgcagg ccgaggggct caatgcgctg cttctaatgc
      841 acgaactttt tggagatgat gacccccgct actggccaca gtttgtcaaa cagtggcagt
      901 ttatcaagca gtaccagatc gaccataata caggcgagtg gttcgacacg gtgtctgccg
      961 aaggccaacc ccagatcaaa gacctggggc acatctggaa ggcagcttat cacaatggta
     1021 gggcactgat ggagtgtggc acgcggcttc gccgcttggc ggggatgaat ggcttaactc
     1081 ctcatgagaa gtccggcaaa agatgacctt ttgttggggg aattttcgac cttgaccggg
     1141 agataaaggt ctttctataa tcgcctgccc gcgcgaaggt gtgttcgtgg cgaaagtcgc
     1201 tccgcgaatt agcgactggg ctagcgattg caatccggcc ggcgcccgcg gcgaaatccg
     1261 gcacgggctc agccaaccca cggtggggca aaggcaaaca agggatgccc acggttttgt
     1321 ttgacctggg gttgatggat aattgacagg aaaggtgacg caaaagagga aggttccgtc
     1381 aacctcaatc tgtgtgctcg gaggcggaac ggcggcaaat ggaattgccc tttgctggtg
     1441 ctgtcatcga agggttgccc acgtgggagc cgatttccac catgccgctc actccctatt
     1501 cggacgtcaa agagcaaatc cggcaggcca tcgatatcgt tgacctcgtg gagcgctata
     1561 tccccttacg tcggcaaggc cggggttttg tggggctttg cccttggcac gacgaccatc
     1621 gcccgagcct tcaagtcaat ccccagcggc agagttttcg ttgttttgtc tgcgatattg
     1681 ggggggatat cttcaccttt atgatgaaga tagaaaatgt cgagtttccc gaggccatgg
     1741 cgatgctggc cgaaatggcc ggtatccctt taccgcgggg catcggcagg ggttccgctg
     1801 ttagtcaaca aaagcaggat ctttaccgcg tcatggcctg ggcggaacag cagtaccacc
     1861 agtgcctttt ggaggctcca gaggcggcac cggcccgcga gtacctggcg agtcgtcaga
     1921 tttcccctgc cagtattgcc caatttcgtc tgggattttc gcctctccag ccggactggt
     1981 tattacgaag ggctcggcgc gatcaaattg atccaaaaga cttggtgaca acgggcatcc
     2041 tcgtgggtac cccgggccgc gatctgatcg accggtttcg cggacgagtt ttattttcca
     2101 tccgcgatag ccaggggcga acggtgggat ttggcgggcg aatcctgccc cacatggcgg
     2161 agcaagtgcc agctaagtac gtcaattccc cggaaacccc cctcttctcc aagagccgcc
     2221 tcctttatgg gctggacgtt gctcgggatg ccttccggcg aacgggtacg gccctagtga
     2281 tggaaggcta caccgacgtg atcatggccc atcaatgcgg ttttcataac gcggtggcgg
     2341 tgttagggac ggccttgacg gatgcccatc tccaggtgct ccaaagattc gtggatcgtg
     2401 tgatcttagt gctggatggg gatgaagccg ggcggcgccg tgcggctcaa gtgttggaac
     2461 tatttgtggc acgacagatt gacctacggg ttttgacgct cccggatgat ctcgacccgt
     2521 gtgactttct cttgaaatat ggggcaccgg gatttcaaga tgcgttggac acacgcacgg
     2581 tcgatgccct cgagcacgcc ttcgccgcgg cgacacacgg tgtggatctc gagcgggaca
     2641 ttgatggtgt cactcgcgct gtcgatcgtt tgctgatggt gatggcgaag gccccggcca
     2701 aactcggtca acacacggct caacagcaag cccgcgaatg gaaaatcctc tcgcggctga
     2761 gcgtgatgac gcggatcgac gaatcggtgc tgcgaaaacg gctagaagag cttcgccgcg
     2821 gtgggcgcag ccgaaccgca gcggtggcca ccccggcacc taaaaccact ctcgatggtt
     2881 ggcatcggac gtttcttgag tttttggtga catttcccga tgcgggggag aagctgcggg
     2941 cagcctttca gccggagcaa attgagattc ccctcgtgcg gagaattttt gaaatcgcac
     3001 ttgatttatt ggccaacggc aaacccgccg atttcgacca tttgatgttg gcagttgatg
     3061 accctctggt gcataatatc ctcgtggatg ttgatcagac ggcgagtgaa aagaggcttg
     3121 ccgagcaaaa tccagaggaa atcctacgtg gtttgttacg ggcccatgag gagcgtgtgg
     3181 ccagggcccg cggtagccaa ttgacccatg tcttaagaca aggaggacta gacccagagc
     3241 aacaaatcga cctactgaag cgaattgtcg aacaagagcg aaatcgccag aagtggtccg
     3301 aacccatgga tgggtgaggc ttttggcgtc aggaagcttg tgactcccgc aagcctgttg
     3361 attcatcggc ttcctgaaag gagtgagtgc tttctttcgg cacggaacgc gcgaaaggag
     3421 acgacccgtg aagcatggcg acaaggagtt gcgtaggctt attgctcaag gacgagccca
     3481 aggttatctc acgtatgatc aagtcatcga atacctcccc gaccaaggtt ctcgaccgga
     3541 aaagcttgat cgcttgatca cggccctgga agaagccggc atcgaaatgg tcaatcgtcc
     3601 gccgcggtcg gagacctcgg caaatgccgc cggagaaccg caagacgaag accccctcct
     3661 caaagatcct cttttgcgag atctgctccc cggagaaaag gccaaatgga acagcgatcc
     3721 catccgtttg taccttagcc agatggccga tatcccccta ctgacgcggc aacaggaagt
     3781 ggccctggca aagaagattg aggttactcg caagagattc cggcgcacgg tgatgggatg
     3841 tcatctggtc ctgaagcaag ccgttcaaac gctggaagcc gttcaacggg gcgatctgcc
     3901 ctttgaacgg accttgaaga tgtccctcac ggaccagttg acaaaaaacc agattttggg
     3961 ccgaatgccc cacaatttac ggacactccg cgcgttgatg gcggaaaacg agaaggattt
     4021 ccgccgcatg atcaaccgtc ggacaccaga agaagtgaag gctaaggctc gccggcgttt
     4081 tctcgcccgt tgggcgagga tgctcattct cgttgaggaa ctcggactcc gcacgcgacg
     4141 tgtccagtcc atgatgcaac aaatgcggga catcttgaaa cggatggaga cgatccgggc
     4201 ccggctgcaa gaactcaaga cgcagcccgg tgaattgggc gaacgcgagc ggcttcgtcg
     4261 ggagctaatg gccctcatgc tatgtaccca ggagacgccg cggagtttgc gaattcgctg
     4321 cgagaagatg gagacgcaat tccgcgatta cagcgaagcc aagcgggaat tgtgcaacag
     4381 taacctgcgg ctggtcgtct ctgtggccaa gcgctaccgt aatcgcggcc tgagtttcct
     4441 cgacctgata caggaaggaa atacgggcct catgcgggca gtcgataaat ttgagtatcg
     4501 ccggggcttt aagttctcga cctatgccac ttggtggatt cgacaagcca tcaccagggc
     4561 gttggcggag cactctcgca cgattcggat ccctgtacac atcatcgacc agattggtaa
     4621 actgaaaaat acgtcgcgcc ggttgatgca tgagctgggc agggagccaa ctcccgagga
     4681 aatggcgcag gccaccgcgt tgccggcaga agatgcgtcc cgcctgctcg aaatgggacg
     4741 tcaacccacc agcctggatc gaccgattgg cgaggacgag gacaattgct tcagtgaact
     4801 gctggctgat cacacgcttg ataagccgga gaacatcgct caccgtgatt tgctgcggga
     4861 gaagattgac aatctgctca aaacgcttac ctaccgtgag cgcgaaatta ttcgcctccg
     4921 ctacggtttg ggcgatggct ataactacac gcttgaagat gtcgggcgta tcttcaaggt
     4981 tacacgggaa cgggtccggc agatcgaagc caaggccatg gaaaaacttc agcatcctgt
     5041 acgggcccag caattggcgc cttttgtgga cggactggtc ggatagtacc atgtccgtcg
     5101 tgggtgacgg atatcaggcg ggctgtccta atggacagcc cttttttatt agttatcaag
     5161 gagatctctc gccatgtcat cgcaagcctt gacaattccc gaaatcttgc gacgcctcca
     5221 cggtctccat cagcagatgg atcaactgcg ggggcgtttg atgaaaggac cacgattagc
     5281 acaagcccat caggagcaga ttcgccaggc cgagaacaat ctcgaccagg ctaagaaggc
     5341 ctacaacgac acccgacttg ccagccatcg caaacagttg gaatacgagc aaaagctggc
     5401 cgacatccaa cgccgcaaag gccgtctttt agagtgcgat gataatcgcg aatatcaact
     5461 gctaaaggac cagatcgctg ctgacgaaat ggcagccagc gttctagcgg atgaagctat
     5521 cgaattgatg acgcgtgtcg aagaaatggc tgtcaaagtc caggaggctg aggctgccct
     5581 ggccgctgct aaggatgccg ccgccaagtg gcaggccgag ttcgagaagc aagaaccggt
     5641 cattcggcaa gagattgctc aggtcgaaca ggaattgaag caccttgaaa cccttttgac
     5701 gggggatgtg cgagagcttt atcaacgggc cctccgcaat aaagggagca gcgccttggc
     5761 acctctcagg ggcgagtttt gtggcgggtg taatcagcat gttcccctac agatgcggag
     5821 tcagttgcta cttggaaatg cgatctggtg ccgctcgtgc ggacgactcc tttatttgcc
     5881 agaaaatgaa aatccccatt cgcggtccta acttgaggga tcgcaggccg gaagcctacc
     5941 agagtcgaaa agttgttctt ctttaattcg gcacggtgga agcgaacgca ggaagactcg
     6001 tccgtagctc ttctgataca cccgggggtc caagatgaca acgatcccac ggtccgcgcg
     6061 ggaacgaatc agcctcccga acccttggcg cagcttaata acggcctccg gcaattgata
     6121 ctcttgaaac gggctgcgtc ctgcggcctt gatcgcttcc aggcgtgctt ctacaagcgg
     6181 acgatcaggc acggcaaagg gtagttttgt gataataacg ttttgcaggg cttcacctgg
     6241 gacatcgacc ccctgccaaa aactgtccgt tccaaacagt acggcccggg gggtggtctt
     6301 aaaccgttcc aacatctgtg atcggggcaa gccctcggcc tgagaaaata gtgtgtagtt
     6361 ttctttggcg aaccaagatt gcagcgcagc agcgattcgc cgcataaagt cataacttgt
     6421 gaagagcacg aaggcacgcc gctctgtcct ggcgatatat cgcttcaagg ccacgaccgt
     6481 tgccttctcg aattgtgaag aatcaaggga gggatcgggc atgtcgcgga caatgatgag
     6541 ctccgcttgc tcttgataat taaaggggct acccacttga agcgtgtccg cccgggtcag
     6601 gccgatccgg ctcttgaagt actcaaaacc cgattctgcc accgctaatg tggcacttgt
     6661 caaaataacg cgcggaattt cctgaaacag gtgctcgcgc aaaattggac cgacatcaat
     6721 gggagcggct ccgagaataa tgcgggggac ttgacgggcc tcctggactt ctacccagta
     6781 cacgtagccc tcgtgggatt gccggatcca ctgatcgacc tgagtggcta acgattgcag
     6841 acggtcccgc acggccacca agtcttgacg ttcctcctct ttttcgatcc gcgctgcttc
     6901 cttgtgaacg atcgaggcga gcttctgcag accttctgtc agcgtattgc gaaagacgtc
     6961 cctttgccgc acccgcccat tgccgggctt ctgcttttcg taccagtcaa acacctgcgc
     7021 gaaaaactcg tcgcaccgat agagacagtc catgacagcc ttttgtcctt ccggacttcg
     7081 ataatggacg aaaagacccc gttgggtccg ctcgttgtaa agcttgcgca gattgaacaa
     7141 gatctgcgtg tagcttacat ccgtcccaaa ttgttccgag gccacggatt ccaacgtgtg
     7201 ggcttcgtca at
//
LOCUS       DAOPTR010000040         7188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_8613_length_7188_cov_3.477219, whole genome shotgun sequence.
ACCESSION   DAOPTR010000040 DAOPTR010000000
VERSION     DAOPTR010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7188
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8613_length_7188_cov_3.477219"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1851)
                     /locus_tag="PKI05_01445"
     CDS             complement(<1..1851)
                     /locus_tag="PKI05_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOA50888.1"
                     /translation="MRSFTRLLVTGIIGAVVGTWCASISQAAGSESTSELFPFVLSYD
                     VADTITNLSDWLERPAGKNGFIRVENGHFVNDRGRVRFWATNLCFEACFPTKEEAERV
                     ARRLASLGINCVRMHHMDNRHIWGKSPNKLTIDPEMLDKLDYLIYQLKQHGIYTNINL
                     HVSRQFGPAEGFPAVEGLPNYDKGIDNFEPKMIEYQKKYARDLLTHVNPYTGNAYVNE
                     PAVAMVEINNENAAFDEYRKGAFDHLPEPYATQLRKLWNSWLRKKYGGDDALRKAWNA
                     QREPLGEEILKLGNFFGNWQGVWNLQRDRLSEVTAEAIPNIREGQPGLRLRVIRNGEQ
                     SWIPQLSQGGFAVKKGQVYTLSFWMRADQSGRIDVNCMMNHDPWQRLGLSGQVEVGKE
                     WKEYRLSFVAEQDDTNARVTFSQLRPGVYELTGVSLRPGGVMGLEQGQSLVNDSIPIV
                     PWRGPQMTPAARADFADFLWELERDYWWGMYRFLKDELKLKSVVSGTQLSYSPVHIQA
                     GLDYIDAHSYWQHPVFPGRPWDPENWYVRNLALVNSGGGTLVGLANRRVEGFAFTVSE
                     YNHPEPNEYAAEGFPMIAAFGAFQDWDGIFSFTYSHSRDFEPRKITGFFDI"
     gene            complement(1964..3298)
                     /locus_tag="PKI05_01450"
     CDS             complement(1964..3298)
                     /locus_tag="PKI05_01450"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA50889.1"
                     /translation="MGTTGRFSRRDFLAAGTGVAVATYVSARALDLEPGQPSANNKLN
                     VAAVGVGGMGKHDIAQAARTENVVAICDVDRRFAEQTAAQYPNARVYVDFRKMLEEQK
                     DIDAVMIATPDHTHAVITAMALKLGKHVFCQKPLTHSVSEALKIRELAAQAKTATQMG
                     NQGQASEAARRICEYIWSGAIGKVREIHSWSNRRPDISPRGIPRPKDTPPVPDYLNWD
                     LWLGPAPERPYHPAYHPFAWRGWWDFGTGVLGDIGCHNLSAAFKALKLQWPISVEACS
                     THWSAPPEITKETAPLASIVTYVFPAEGDRPEITLRWYDGGMMPPIPKELGDRNIFDG
                     DGTLIVGDKGMLWGDRLLPESLAQEIGDPPKMLPRSPGHYEEWIAACKGGPAAGSNFV
                     DHAAHLAAVVLMGNIAIRTQKKLFWDAEKLRFTNSEEANALLDPPYRNGWSL"
     gene            3744..4586
                     /locus_tag="PKI05_01455"
     CDS             3744..4586
                     /locus_tag="PKI05_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOA50890.1"
                     /translation="MKLTRRDCLRLSGTAGLLLACGQAPLSAFERKKIPVGVQLYSVR
                     QDAAKDLPGVLKAIKEMGYDGVEFAGYYGHDAKSIRKWLDDNGLKCCGTHTGWDTLQP
                     DKLAETIEFNQIIGNKYLICPWLNPAEFAASREKTIEFARRFNELAEKVKEKGMLVGY
                     HAHGGDFKKLNGETAWEIFFSHTVPEVVMQMDIGNCLGGGGDPYAILEKFPGRSKTIH
                     LKEHGGPKGAVIGEGEVKWDWVFNFCETKGGTEWYIVEQESYAVPPLEAIRKCRENLR
                     KMGI"
     gene            complement(4707..5405)
                     /locus_tag="PKI05_01460"
     CDS             complement(4707..5405)
                     /locus_tag="PKI05_01460"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOA50891.1"
                     /translation="MRRIGQSLVILAFVLTVSGSAAHAGLVWLDPNNFSEGQQITVPF
                     VTLSAVQGSTPQLRPDGRVLALSDSNYPAIRFFGWHIAGATQDGEQVPWRAKWANFQA
                     EFESPVSYVSLDFYRNDFGIGTTADDIGFLIALDASNQIIWNEWVYLSGTEESATVEI
                     SLPEPQIKTILAGGVYVRSRANCNPGFDHDILITRLGYSVQPIPEPGTAVLLLSGAVI
                     VGAIARFRRSKRSN"
     gene            complement(5718..>7188)
                     /locus_tag="PKI05_01465"
     CDS             complement(5718..>7188)
                     /locus_tag="PKI05_01465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA50892.1"
                     /translation="GGPVVAAILTVTMILLATRTRLLFSPSEKREFVHFWKQLTGLHQ
                     ALRSFLVGGRWHRQNPPKALPLDTDARTSCLEPAFVGKAGRAWPNPFFRRKRHPPLGD
                     RGPLRVMFVITSMPVGGAERLLVEIIRRLDRRIIQPELCCLKTLGPLGEELAQEVPAF
                     SQLLSHKYDLRVLPRLYLLMRRRHIDAVITVGTGGDKMFWGRLAAFLAGVPVIASALH
                     STGLPDRVEWLNRLLTPLTDAFIAVAQPHAEYLVAHEGCPRDRTWIIANGVDTDRFSP
                     RPPSPERRRELGLPPGCPVVSIVAALRPEKNHELFLQAAAIIHKAIPEAHFVIVGDGP
                     RREMLEQMATQLGLQEVVHFLGTRHDIPDILACSDVVVLSSHMEANPLAVLEAMACGK
                     PVVATRVGSLPTNVVDGLTGFLVPPGDVVVLADRCMLLLNSARLREDMGRAGRRHVLN
                     RASLTHTVKGYEGLIVKLYARKCGSCASTWGNNTHREVY"
BASE COUNT         1689 a         2059 c         1939 g         1501 t
ORIGIN      
        1 gatatcaaaa aagcctgtga tcttccgcgg ttcgaaatcg cgactgtggc tgtacgtaaa
       61 actaaagatc ccatcccagt cttggaaagc accaaatgcg gcgatcatcg gaaagccctc
      121 ggcggcgtat tcgttgggtt cgggatgatt gtactcgctt acggtgaaag caaatccctc
      181 cacgcggcga ttggccagac cgaccaacgt ccccccgccg ctgttcacta aagccaaatt
      241 gcgcacatac cagttttccg ggtcccacgg tcgcccggga aagacgggat gctgccagta
      301 agagtgggca tcgatgtaat caagccctgc ctggatgtgg acggggctat acgaaagctg
      361 cgtgcccgaa accacggact tcaacttcaa ctcatccttt agaaaccgat acatgcccca
      421 ccagtaatca cgttctagct cccacaagaa gtcggcaaaa tcggcccgcg ctgccggcgt
      481 catctgaggg cctctccaag gaacgatggg gatcgagtcg ttgaccagcg attgcccctg
      541 ctccagtccc attacgcccc ctggccgcaa cgagacaccg gtcagctcat agacgcccgg
      601 ccgaagttgg ctaaaagtca cgcgggcatt ggtatcatcc tgttccgcaa cgaagctcaa
      661 tcgatactct ttccactcct ttccaacttc gacctgacct gacaaaccca gccgttgcca
      721 gggatcatga ttcatcatgc agttgacatc gatgcgtccc gattgatctg cccgcatcca
      781 aaagctgagc gtgtacacct gtcccttttt cacggcaaag ccaccctggc taagttgggg
      841 aatccaggat tgctcaccat tgcgaataac ccttaaacgc aggccaggtt gtccctcacg
      901 aatgttggga atagcctccg ctgtcacctc ggaaaggcga tcgcgctgga gattccacac
      961 gccttgccag ttaccaaaga aattgcccaa tttcagaatt tcctcgccca atggttcgcg
     1021 ctgagcattc caagctttgc gaagagcatc atcgccacca tactttttcc tcaaccagct
     1081 attccacagc ttgcgcaatt gcgtggcgta tggttccgga agatgatcga atgccccctt
     1141 acggtactcg tcgaacgcgg cgttttcgtt gttgatttca accatcgcaa cggccggttc
     1201 attgacgtag gcgttaccgg tgtaggggtt cacatgagtg aggaggtcgc gggcatactt
     1261 cttttgatac tcgatcatct tcggctcaaa attgtcgatc cccttgtcgt aatttggcag
     1321 gccctcaacc gcaggaaacc cctccgctgg tccaaactgc cgagagacat ggaggttaat
     1381 attggtataa atgccgtgtt gcttgagctg atagatgaga tagtccagtt tatcgagcat
     1441 ctccgggtca atggtcaact tgttagggct tttcccccaa atatgccgat tatccatgtg
     1501 gtgcattcgc acacaattga ttcccaagct ggcaagacgc cgcgcgactc gttccgcttc
     1561 ctctttggtc ggaaagcatg cctcaaagca aaggttcgtg gcccaaaagc ggactcgacc
     1621 acggtcattc acaaaatgac cattttctac ccggatgaaa ccattctttc cggcgggacg
     1681 ctccaaccaa tcagaaaggt tcgtaatcgt atcggccaca tcgtaactta gcacaaaggg
     1741 gaaaagctcc gatgtactct cagaccctgc tgcctgacta atcgaagcgc accaagttcc
     1801 caccacagcg ccgatgatcc cggtaaccag taagcgcgtg aaagacctca cggcgagacc
     1861 tcctttcgta tcctggtgat ttgagtaagg gaaggacgta aaacgactcg cgcactctca
     1921 gctccctgcg cgaggctcgg acgaactcag tgatgattat agatcacaac gaccaaccgt
     1981 tgcgataggg tggatcgagc aacgcgttgg cttcctcgga gttcgtgaat cgcaatttct
     2041 cagcatccca gaaaagcttc ttttgggtgc gaatggcgat gttccccata aggaccaccg
     2101 ctgccaaatg tgcggcatga tcgacaaagt tactccccgc cgccggacca cccttgcagg
     2161 ccgcaatcca ttcctcatag tgaccggggg accgcggaag cattttaggg ggatcaccaa
     2221 tttcctgggc gagggactcc ggcaagaggc gatcgcccca gagcatgccc ttgtcgccga
     2281 caatcaaggt accgtcgccg tcgaagatat tgcgatcccc aagttcctta ggaatcggcg
     2341 gcatcatgcc cccgtcgtac catcggagtg tgatttccgg gcgatctcct tctgcgggga
     2401 agacatacgt gacaatcgac gccaagggtg ccgtttcttt tgtgatctcc ggaggtgcgc
     2461 tccaatgggt cgagcaggcc tccacagaga tgggccactg gagcttcagg gccttgaacg
     2521 ccgccgaaag attgtggcat ccgatgtccc caagaacgcc ggtgccaaag tcccaccaac
     2581 cgcgccaagc aaaagggtga tacgctggat gatacggccg ttcgggcgcc gggcccaacc
     2641 acaaatccca attcaggtaa tcgggcacag ggggtgtgtc ctttggacga ggaatcccgc
     2701 ggggcgagat atcgggacgg cgattcgacc aagaatggat ttcacgaacc ttcccgatgg
     2761 cgccggacca gatatattcg caaatgcgtc gggcggcttc cgaagcctga ccctgattac
     2821 ccatctgcgt ggccgttttg gcttgggccg ccagttccct gattttgagt gcttcactta
     2881 cgctgtgcgt gagtggcttc tgacaaaaaa cgtgttttcc cagtttgagg gccattgccg
     2941 taataacggc atgcgtatga tcgggcgttg cgatcatcac ggcatcgata tccttctgct
     3001 cttccagcat cttgcgaaaa tcgacataca cgcgagcgtt tggatactgt gccgccgttt
     3061 gctccgcgaa gcgacgatcg acatcgcaga tggctacgac attttccgtc ctggccgcct
     3121 gggcaatatc gtgtttgccc atcccgccca caccgaccgc cgccacgttc agcttgttgt
     3181 tggcagaagg ctgtcctggt tcaaggtcca aggcccgggc ggaaacatag gtggcaacag
     3241 ccactcccgt ccccgcggcc aaaaaatccc gtcgggaaaa gcgaccggtt gtccccatga
     3301 taaaccctcc caatcaagca actcttactg accaattccc gttgcgacgg cattgtaaat
     3361 gatgcgcact cgaaatgaaa gcaaggtata tcgacccgcc aggttgcgca gctgactccc
     3421 gagatatctt tcgcccgcgc cccagaaacg cacgccgcgg cgcatctctg cagattcaac
     3481 aattgggtaa ccacaagggc cgatgccaac caacgcccga cctcctcatg aagggctaca
     3541 ccatgtcgtg gccacagtgg acgacggagg cgttttttga gaaggtgtaa cttcttccac
     3601 caccagtcgg ggcgggtccc tctggcagtc cgttatcgac cccatttgct tagggccggt
     3661 ccgacacatt atcatgatca gtcgttgtgg ctctagaagc cgaaaggcga gtcttggtta
     3721 gcaacttgtg aggaggagaa acaatgaaac tgacacgacg cgattgtttg cggttgtccg
     3781 gtacggcggg attgctactc gcatgtggcc aggcgcccct gtctgccttt gagcggaaaa
     3841 agattccggt gggcgttcaa ctctattctg tccgtcagga cgccgcaaaa gacctgccgg
     3901 gtgtgttaaa ggccatcaaa gagatgggct acgacggcgt ggaattcgcc ggctactacg
     3961 gacacgatgc gaagtccatt cgcaaatggt tggacgacaa tggtttgaag tgctgtggta
     4021 cccataccgg ttgggacacc cttcagcccg ataaattggc cgagacaatc gaattcaatc
     4081 agatcatcgg caacaagtac ctgatttgtc cgtggcttaa cccggccgag ttcgcagcca
     4141 gtcgagagaa gacgattgaa tttgcccggc gattcaatga acttgcggag aaggtcaagg
     4201 aaaagggaat gctggttggc tatcatgcgc atgggggcga cttcaagaag ctaaacgggg
     4261 aaacggcctg ggagatcttt ttcagccaca cggttcctga ggtggtcatg caaatggata
     4321 tcgggaattg cttgggcggt ggaggagacc cctacgcaat cttggaaaaa ttcccgggac
     4381 gatcaaagac gattcacctt aaggaacacg gcgggccaaa gggggcggtt atcggtgaag
     4441 gcgaggtcaa atgggactgg gtgttcaact tctgcgagac caagggtgga accgagtggt
     4501 acatcgtgga acaggagagc tacgccgtgc cacctctgga ggccatccgc aagtgccgcg
     4561 agaaccttcg caagatgggg atttaacgta tcatcccgta ttgtgacagt catgctttga
     4621 tgcccccaac cgcagggcga aaaaaacggg agcgtcatgt gacgctcccg tttggctttc
     4681 gtccctggca ggattgctac aggtgtctag ttacttctct tgcttcgacg gaatcgggca
     4741 atagccccca caatgactgc cccgctgaga aggaggacgg cggtacccgg ttcaggaatc
     4801 ggctgtacac tataacccag tcgggtaatc aggatgtcgt gatcaaaacc cgggttacag
     4861 ttggccctgc tccgaacgta cacgccacca gccaggatcg ttttgatttg cggttcgggt
     4921 aaagagattt caacggtagc cgattcctca gtccccgaca gatacaccca ctcgttccaa
     4981 atgatctgat tcgacgcatc aagtgcgatg agaaaaccga tatcgtccgc ggtagtgcct
     5041 atcccaaaat cgttccggta aaaatctaaa ctgacataag acaccgggct ttcaaactca
     5101 gcctggaaat tcgcccattt tgcccgccac ggaacctgct cgccatcctg ggttgcgccg
     5161 gcaatgtgcc agccaaagaa acggatcgcc ggatagttac tatcggaaag cgcaagcacc
     5221 cgaccgtcgg gtcgaagttg aggggtactt ccttggacgg ctgacaacgt tacaaacggg
     5281 acggtaatct gctgcccctc gctaaaattg ttgggatcga gccacacaag ccccgcgtga
     5341 gcggcgctgc ccgataccgt aagcacaaat gccagaatta ctaaactttg gccaattctc
     5401 cgcatggtat gcctcttctc ttgcccctaa agttcaccag gatgcctgac cgctgttgct
     5461 tgatctcaaa cgcccccctc aatggaggca gccaccttat ccatgttact aagagggaaa
     5521 cttggaatca atagcaaaat aacgaaaata cgaactctac tggatatggc ataaatacgc
     5581 aagactcatc acccattgga cgcgattatc gtgcaattag ttccaacgct aatgcgacgc
     5641 atcaggatga atgctcccgg gggcgccgcg accgttcgcc gagaggcctt ctctcaaccg
     5701 cggggcaggc ccatcgctca gtacacctcg cgatgggtat tattccccca agttgatgca
     5761 caagagccgc acttacgggc atacaacttc acgataagcc cctcgtagcc ttttacggta
     5821 tgcgtgagcg aagcccggtt taggacgtgc cggcggcctg cacgtcccat atcttcgcgt
     5881 aatcgggcac tattgagcaa gagcatacac cggtcggcaa gcaccaccac atcgcccggg
     5941 gggacgagaa acccggtcag gccgtctacc acatttgtgg ggagcgaccc aacccgcgtg
     6001 gcgacaaccg gtttgccgca ggccatcgct tccagaacgg ccaacggatt ggcttccatg
     6061 tgcgaagaaa gcacaaccac gtcggaacat gccaagatgt ccggaatgtc gtggcgggtc
     6121 cccaaaaaat ggacgacctc ttgcagccca agctgagttg ccatttgctc cagcatttcc
     6181 cggcgaggcc cgtcgcccac aatgacaaaa tgggcctcag gaattgcttt gtggataatt
     6241 gcggccgcct ggagaaaaag ctcgtggttc ttttccggac gcagggcagc tacgatcgac
     6301 acaaccggac atcctggggg taaccctagc tctcgacgcc gctccgggga gggtgggcgc
     6361 ggcgaaaacc ggtcggtatc gactccatta gcgatgatcc aagtccggtc gcgcgggcag
     6421 ccctcatggg caacgagata ctcggcatgg ggttgggcaa ccgcgatgaa tgcatcggtc
     6481 agcggagtaa ggagacggtt cagccattcg acccgatcgg gcaagcctgt cgaatggagt
     6541 gctgacgcta tcaccgggac accggccaag aacgcggcca gccggcccca aaacatcttg
     6601 tcaccacctg ttccaaccgt gatgacggca tcgatgtgcc gccttcgcat gagcagataa
     6661 agccgcggca agacccgcaa atcgtacttg tgactgagaa gctgtgagaa cgccggaact
     6721 tcctgagcga gctcctcacc tagcggacca agggtcttca aacaacacaa ttcgggctgg
     6781 ataatgcggc gatcaaggcg gcgaataatc tccacaagca gccgttcggc tcccccaacc
     6841 ggcatcgagg tgatgacgaa cataacgcga agtggtccgc ggtccccgag cggtggatgc
     6901 ctttttcttc ggaaaaatgg gttaggccag gcccggcctg ctttccccac aaaggccggt
     6961 tccaagcagg aggtgcgcgc gtcggtgtcg agaggcaacg cctttggagg gttttgccga
     7021 tgccatcgcc cacctactag gaaactgcga agagcttgat gcagccccgt tagttgtttc
     7081 caaaaatgca cgaactcgcg tttctcgctt ggggagaaaa ggagtcgtgt ccgcgtagcg
     7141 agcaggatca ttgtcacggt gagaattgct gccaccacgg gtccaccg
//
LOCUS       DAOPTR010000041         7139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_8678_length_7139_cov_4.000141, whole genome shotgun sequence.
ACCESSION   DAOPTR010000041 DAOPTR010000000
VERSION     DAOPTR010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7139
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8678_length_7139_cov_4.000141"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PKI05_01470"
     CDS             <1..339
                     /locus_tag="PKI05_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HOA50893.1"
                     /translation="DFNQFIRLDESALATIFEKADDELVVLSLWGAPLGLVEKVLQQF
                     PTWRAEAIRRELADLGPVRLSDVEEARRRLAEYASKLAACRRIRLPPELQSHPVATGS
                     ARSPGSTYGL"
     gene            404..1072
                     /locus_tag="PKI05_01475"
     CDS             404..1072
                     /locus_tag="PKI05_01475"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HOA50894.1"
                     /translation="MEARSSVIKVHDGAGVAQATPFNFEDMAERARAYLDGVRKQAVQ
                     IVAQAQKEAAEIRKRAEIEGRRSGTEQAERVIRSELEKSLTTVLPALREAVSQINQAR
                     QAFLAEWEKSLLRLAGAIAVKLVRQAVIETPEVAVPLIRETLELAAKAPRVRIRLHPE
                     DEKALRPVVTKILQELSPAAVGEVLPDPAITRGGCRVETDFGSIDQQFEKQVQRIIEE
                     LTGQ"
     gene            1103..2494
                     /locus_tag="PKI05_01480"
     CDS             1103..2494
                     /locus_tag="PKI05_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HOA50895.1"
                     /translation="MIDLKSHLERIISAEIEGSVTQSLGITMIAHGFSVPVGSLAEIE
                     IPHGGNVLAEVIGFKNDSTILFPLGRPEGVRPGCRVRLARTLHWLRVGNDLLGRVVNA
                     QGHVIDGKAQPFLKHRVLFDQPAPHPCERPRIQDPLPTGIKAIDGLLTCGRGQRLGIF
                     AAAGVGKSVLMGMMARYTAADVIVIGLIGERGREVNDFIERDLGPEGLKKSVVVVATS
                     SEPAILRVQAAYAATAIAEYFRDQGKDVLLLMDSLTRFALAQREIGLAAGEPPTTRGY
                     PPSVFALLPRLVERAGRTPRGSITAFYTVLVEGNDPDEPVADAARGLLDGHIWLSRSL
                     ANRGHYPAIDVLESLSRLMPDVVEPRHYQSALQVRDLMGVYREHEDLITIGAYRRGSH
                     PKVDLSIQLRDTINAFLRQNVGQRAEFNETRDSVIKLGEQIQETLRAATTQQSGKEST
                     GRHAGVNGPPVRS"
     gene            2546..2998
                     /gene="fliJ"
                     /locus_tag="PKI05_01485"
     CDS             2546..2998
                     /gene="fliJ"
                     /locus_tag="PKI05_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HOA50896.1"
                     /translation="MRRFTFRLETVLRIREQERDERKRELEQAYAARDILLRQRDALE
                     AERQHLGAALRESLQQGSIDADQMLAWRRYEAVLNAQREYLTKQLSAIEGEIEKRQLA
                     LMEANREVRILEVLRDKQRTAHEKQERAREGKKLDEIANRRSHGLREP"
     gene            2995..3645
                     /locus_tag="PKI05_01490"
     CDS             2995..3645
                     /locus_tag="PKI05_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50897.1"
                     /translation="MMRFLETILSVLLYFFAAMAIGALCLFIHLWFAWKLTPEKIRTA
                     IAAARGTLPPPVTAIAGPVGPKEPAEEPSYSDILAKRASSYRDLELKEKMLASLKDEL
                     LQWETTLSKKEEVLGQQEKAFQERLAQMKAEAESAGLEAVRRTLESLRPTQAKQLIEE
                     MLAQKEEDQVVTLLQGMSDSKRARIIAEFRTEEEMARIAEVLRRLRQGGELGTSQQ"
     gene            3854..5392
                     /locus_tag="PKI05_01495"
     CDS             3854..5392
                     /locus_tag="PKI05_01495"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOA50898.1"
                     /translation="MGSSFWNVLASEIQGTEESSRPSVTARCAETIPGGTKNGAHQGE
                     SVADTRDEKLIEPCPGKMPKEGAAQEHDQALHDDSDVERSGKNSGEGAALGQDAEPQT
                     STNPYQVVVCCQKGQGAEPGTLVADTLVKESAPETSHMQDKASDVTADVVGIGQSVLP
                     LTVQLITSEENSTDQIEETGLGFESEVKPFGKKLNKGGPQAQIMPSAVPSSEAVEEAN
                     QTGGTVNQERATGDSETVSEQLRSAFPAVAVSGGQSDVIGQTVSRKKESAVLSGQDNG
                     ANPTVSQKLTSGTTEMTPQNAGLVTSYDPSVNASLAVSNERDERVKTVEGAKQEGAGF
                     VFTRKDLARTEVSTGPKTGEQSLAQGPSMRADQAEQIITVQRVAQAIRLAHERNGEIR
                     LRLHPPELGALRLQMRVQEGVLTARLEVETLAAREVLLDNIAGLRERLAEHQIRVEHF
                     DVALMNDSLGGQTKGREEFLGSSHPSRHRGASAEPKSSEEVGGQIPSIPTRRLGDRQF
                     DVFI"
     gene            5443..5850
                     /locus_tag="PKI05_01500"
     CDS             5443..5850
                     /locus_tag="PKI05_01500"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HOA50899.1"
                     /translation="MIVNSAAATRQATNQNPTGHDAFRDLDLNSFLSMMIAELQNQDP
                     LNPMDNAQLLQQVSQIREIESNLRLVSTLESLVRAESLASGTNLIGRTVKGLDTNGKE
                     VVGVVEKVSADEKGFYVHFDNTTVSMRNIKEVI"
     gene            6003..>7139
                     /locus_tag="PKI05_01505"
     CDS             6003..>7139
                     /locus_tag="PKI05_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HOA50900.1"
                     /translation="MGLASALTTALTGLTAAETTIDVVGNNLANSNTVGFKASSAAFA
                     TQFLQTLALGAMPTDNTGGTNPRQIGLGTMVADITPNFNPGTIEISSNPRDIAIQGDG
                     FFIVEGQQNERLYTRNGIFKLNSQNQLVTITGNRVLGYGIDDNFQVQRTELEPITIPI
                     GSEAVAKATENVYLEGTLSPTGDLATTASRIQTPILGDGRYTRPETQLSPGYSPEPNV
                     SGTLLSSQSTGTGGLVAGTTYEYKIVFAHGQYLPGPPNDPPAMSEGMYSSTITGTVGA
                     GGDSLLISNLPTDPSGNYDYIRIYRRVSGETEFKYVDEVPMGTASYLDETPDANLGPA
                     MDTTLLSGNFTYYVAFEKDGRISRPSPISDPINILNGRVVLKDLP"
BASE COUNT         1748 a         1822 c         2128 g         1441 t
ORIGIN      
        1 gatttcaacc agttcatccg tctcgatgaa tcggccctgg cgacgatttt tgaaaaggct
       61 gatgacgagt tggttgtcct ctcgctttgg ggggctccct tgggccttgt ggaaaaagtg
      121 ctccagcagt tcccgacgtg gagggctgag gccattcgcc gggagctggc cgatttggga
      181 ccggtccgac tgagtgatgt tgaggaggcc cgccgccgcc ttgcagaata cgccagcaag
      241 ttggcggctt gtcgccgcat ccgactgccc ccggaattgc aatcgcatcc ggtggctacc
      301 gggtcggcac gttcgccggg gagtacttac gggctctgat gcagactgtg tcttgtggga
      361 gaaaaggcat ctgatagcga agtgagtttt ggttgggatg ccaatggaag ctcggtcctc
      421 agtcatcaag gttcacgatg gtgcaggagt cgcccaggcg acgccgttca attttgaaga
      481 catggccgag cgcgccaggg catacctgga cggcgtgcgg aaacaggccg tccagatcgt
      541 tgcccaggcc caaaaagaag cagccgaaat tcgtaaacgc gccgagatcg aaggacgacg
      601 atcagggacg gagcaagcag aacgggttat tcgcagtgaa ttggagaaaa gccttaccac
      661 ggttctccct gccttgcggg aggccgtatc acagatcaac caggcgaggc aggcatttct
      721 ggcggagtgg gaaaaatcgc ttttacgctt ggcgggggcc atcgccgtta agctcgtgcg
      781 gcaagctgtg attgaaaccc ctgaagttgc cgtcccgttg attcgcgaga cgctcgagct
      841 ggcggccaag gccccgcggg tgcgaatccg attgcaccca gaagacgaaa aggctcttcg
      901 ccctgttgtg acaaaaatcc ttcaggaatt atccccggcg gctgtgggcg aggtcttgcc
      961 tgatcccgcg atcacgcggg ggggatgtcg ggttgagacc gactttggct ccatagacca
     1021 acagttcgag aaacaggtgc aacggatcat cgaggaattg acaggccaat gaaaacattg
     1081 gggaagactg cgtgccaaga aaatgattga ccttaagagc catttagagc gaatcatttc
     1141 cgcagagatc gaaggatcgg tgacgcaaag cctcggcatc accatgatcg cccacggctt
     1201 ttcggtccca gtggggtctc ttgcggaaat cgagattcct catggcggta acgtgttggc
     1261 ggaggtcatc ggattcaaga acgactcgac aattttattt cctttgggtc gtccagaagg
     1321 tgtgcggccg gggtgtcggg tacggttggc caggacactg cattggttac gcgtggggaa
     1381 tgacttgctc ggccgggtgg tgaacgccca gggccacgtg atcgacggca aggctcagcc
     1441 gtttcttaag caccgtgtcc tttttgatca accggcaccc cacccgtgcg agcgccccag
     1501 gattcaggat ccgcttccca cggggattaa agccattgac ggtcttctca cttgtgggcg
     1561 gggacagcgt ctcggcattt ttgcagcagc cggggtgggc aagagtgtcc tgatgggcat
     1621 gatggcccgt tatacggcgg ccgatgtcat tgtcatcgga ctcattggtg agcgggggcg
     1681 ggaggtcaat gactttatcg aaagggattt ggggccggaa ggcctgaaga aaagcgtggt
     1741 agtggttgcc actagtagcg aaccggccat tttgcgagtc caggcggcct acgcagccac
     1801 ggcaatcgcg gagtattttc gggatcaggg taaggatgtg cttttgctca tggactccct
     1861 tacgcgattc gccctggccc agagggaaat cggactggcc gccggcgaac cccctacgac
     1921 acgcggttat ccgcccagtg tttttgccct tctcccgcga cttgttgagc gggcaggacg
     1981 aacaccccgg ggaagcatca cggcgttcta caccgtgttg gtggaaggta atgaccctga
     2041 cgaaccggtg gctgacgcgg cacgcggatt actcgacgga catatttggc tctcgcggag
     2101 cttggccaac cgagggcatt atccagccat cgatgtcttg gagagtctta gccggctaat
     2161 gcccgacgtt gttgaacctc gacattatca gtccgccctg caggtgaggg acctgatggg
     2221 tgtttacagg gaacacgaag atttgatcac aattggagcc taccgtcggg gcagccatcc
     2281 gaaggtcgat ctttccatcc aactaaggga tacgatcaac gctttccttc gacaaaacgt
     2341 gggtcagcgg gcggaattca atgaaaccag agacagcgtg atcaaactgg gtgaacaaat
     2401 tcaggagacc ttgcgggccg ctacgacgca acagtccggg aaggagtcca ccggacggca
     2461 cgctggggtc aatggaccac cggtgcgttc atgaatcgga aactttgatc aataaacaag
     2521 gacactaaat ggtatccaaa cctccatgcg ccgatttacc ttcagactgg aaactgtact
     2581 acgtatccgt gaacaggaaa gggatgaaag gaaacgagaa ctagaacagg catatgcggc
     2641 gcgagacatc cttctgcggc agcgggatgc actggaggcg gagcgccagc atttgggagc
     2701 ggcacttcgg gaaagtttgc aacaaggcag tattgacgcc gatcaaatgc ttgcttggcg
     2761 acggtatgag gccgtattga atgcccagcg cgaatacttg accaaacaac tgtcggcgat
     2821 cgagggagag attgaaaaac ggcaactggc actgatggag gccaaccggg aggtccgcat
     2881 tttggaagtt ttgcgcgaca agcaacgaac cgctcacgaa aagcaggaaa gggcacggga
     2941 agggaagaaa ctggatgaaa tagcaaaccg cagatcgcac ggcttgcgcg agccatgatg
     3001 aggttcttgg agaccatact ttcggtgctt ctctacttct tcgccgcaat ggcgatagga
     3061 gcactctgtc ttttcattca cctttggttc gcatggaagt tgacccccga gaagatacgc
     3121 acagccatcg ccgcggcccg cggaaccctt ccgccgccgg ttaccgcgat tgccgggccg
     3181 gtcggtccca aagagccagc cgaggaacct tcttatagcg atatcctcgc caagcgagcg
     3241 agcagctacc gcgatctgga actgaaagag aagatgctcg cttccctcaa ggatgagctt
     3301 ctgcaatggg aaacgacgtt gagcaaaaaa gaagaggttc ttgggcaaca ggaaaaggca
     3361 ttccaggaac gcttggccca gatgaaggcc gaggcggaaa gtgccggatt ggaggccgtg
     3421 cgacgaacct tggaatccct tcgcccgact caggccaagc aattaatcga ggaaatgttg
     3481 gcacaaaagg aagaggacca agtggtcacg cttttgcaag gcatgagcga ctctaagcgt
     3541 gcccgcatca ttgcagagtt ccgtaccgag gaagaaatgg ccaggattgc cgaggtcttg
     3601 aggcgtctcc gccagggcgg ggaattggga acttcccagc agtaactgga ttcttgggag
     3661 gtcgcgatcg ggagggcggc agaatgattt agatacagct gaacacaata cggaatatta
     3721 caactaacac aatcacaaga actggcaaca tatcatgctc gacagaatcg ggttcgctcc
     3781 tgaaataggc attatcgatt cgttgtctgc tatccagaca agaggcctag gattctctga
     3841 ccaggctgag gaggtgggtt ctagcttttg gaacgttttg gcatcggaaa ttcaggggac
     3901 ggaagagtcc tcgcgcccta gtgtcactgc tcgatgtgct gaaacgattc cgggtgggac
     3961 aaaaaacggg gcccaccagg gagaaagtgt tgctgatact cgcgacgaga agcttattga
     4021 gccttgccca gggaaaatgc caaaagaagg tgctgcccaa gagcatgacc aagctttgca
     4081 cgatgacagc gatgtcgaga ggtcgggcaa aaacagtggc gagggtgccg cgcttgggca
     4141 ggacgcggag ccccagacgt ccacgaatcc ctaccaggta gtcgtttgct gtcaaaaggg
     4201 ccagggggcc gagcctggca cgctcgtcgc agacactctt gtaaaggagt ccgctccaga
     4261 gacatcccac atgcaagaca aagcgagcga tgtgacggcg gatgttgtcg gaatagggca
     4321 gagtgtttta cccctcacgg tgcagttgat tacgagcgaa gaaaatagta ccgatcagat
     4381 tgaggaaacc ggattaggtt ttgagtcgga agtcaaacct ttcgggaaaa agctgaataa
     4441 aggtgggccc caggctcaaa taatgccgag cgcggtacca tcttcggaag cggtcgaaga
     4501 agccaatcaa accgggggaa cggtcaacca ggagcgggca acgggggata gtgagaccgt
     4561 atccgagcaa cttcgttccg catttcctgc cgtagctgtg tcagggggac agtcagatgt
     4621 tattggtcaa accgtttcgc ggaagaaaga atcggcggta ctttcagggc aggacaatgg
     4681 ggcgaacccc accgtaagcc aaaagttgac gagtggtacg acggagatga cgccacagaa
     4741 cgccggcctt gtcacttcct acgatcctag cgtcaatgcc agtctggcag tgtccaatga
     4801 gcgggatgaa agggtaaaaa cggtcgaagg ggcgaaacaa gaaggggcgg ggttcgtctt
     4861 cacccgaaag gatttagcca ggacagaggt ttccacgggg ccaaagacgg gtgaacaatc
     4921 tcttgcgcaa ggtccctcga tgcgtgcgga tcaagctgag cagataatca ccgttcagcg
     4981 cgtggcccag gcgatacgct tggcacacga gcgcaatggt gaaattcgcc tgagactcca
     5041 tccgccagag ttgggtgcgc tgagacttca gatgcgagtc caggagggag ttctcacggc
     5101 ccgtcttgaa gtggaaaccc tggcggcgcg ggaggtcctt cttgacaaca tcgccggact
     5161 gcgagagcgt ctcgctgagc accaaattcg cgtagagcat tttgacgtgg cactgatgaa
     5221 cgattctctc ggaggccaaa ccaagggccg tgaggaattc ctgggttcat cccacccgtc
     5281 gcggcaccgg ggtgcctcgg ctgagccgaa gtcctctgaa gaagtcggcg gccagattcc
     5341 cagtattccc acgcgtcgcc tgggagaccg ccaatttgat gttttcattt gagatggaac
     5401 aagacagtca gcgatctgaa atctgttgag atggagtgaa caatgatcgt taactcagca
     5461 gctgcgactc gccaagccac gaaccagaat cctaccggtc acgacgcctt tcgcgacctg
     5521 gatctcaata gtttcctcag catgatgatt gcagagttgc agaaccaaga ccccctcaat
     5581 cctatggaca atgcgcagct cttgcagcaa gtcagtcaaa ttcgggaaat cgagtctaac
     5641 ttgcggctcg tgagcacgtt ggaatcgcta gtccgcgccg agagtctcgc cagcgggacc
     5701 aatttgattg gccgaaccgt caagggactg gatacgaacg gtaaggaggt cgtgggagtt
     5761 gtagaaaagg tctcggctga tgagaagggc ttctatgtgc actttgataa cacaacagtt
     5821 tccatgagaa acatcaaaga agttatctaa agaggggatc gctcacgaac gaatacctgt
     5881 gatgaatcaa ctttgattta cgaatcggag ggcgtcgaac ggtgacggag atcaaactag
     5941 atacacgttg aaagagacgc tctgtggggg attgtcaata gcgagtccaa ggagccatag
     6001 atatgggtct agcctccgca ctcacaaccg cgttgacagg cttaacggca gcggaaacca
     6061 ccattgacgt ggtcggcaac aatctggcca actccaacac cgttggcttc aaggcctctt
     6121 cggcagcgtt tgccacccag ttcttgcaaa cgctggccct gggggcgatg cctacggaca
     6181 acacgggagg aaccaatccc cgccaaatcg gcttgggaac aatggtggct gacattaccc
     6241 ccaatttcaa tccggggacg attgaaatta gctctaaccc aagggatatt gccatccagg
     6301 gtgatggctt cttcattgtg gaaggtcagc agaatgagcg actctacacg cgaaatggca
     6361 ttttcaaatt gaattcccag aaccaactcg taacgatcac aggaaatcgg gtgctagggt
     6421 acggcattga cgataatttt caggtccagc gcaccgagtt ggagccgatc acaattccca
     6481 ttggttccga ggccgtggcg aaggccacag aaaatgtcta tttggaaggt accctatctc
     6541 caaccggtga tttggctacg acggcgtcgc gaattcaaac acctatcctg ggtgatgggc
     6601 gttacactcg tcccgaaacg cagttgagcc ccggatattc cccagagcca aatgtttcgg
     6661 gaactcttct ctccagtcaa tctacgggaa caggtggcct cgtagccggg accacctacg
     6721 aatacaaaat tgtgtttgcc catgggcagt acctccccgg gccgcccaat gacccgccgg
     6781 caatgagtga ggggatgtat tccagcacga tcacgggcac agttggggcc ggtggtgact
     6841 cgctactgat ttccaatctg cccactgatc ccagtgggaa ttatgactac attcgcattt
     6901 atcgccgcgt gtcgggggaa acagaattca aatatgtgga tgaggtcccc atgggcacgg
     6961 caagctattt ggacgaaacg cccgacgcca acctcggtcc agcgatggac acgacattgc
     7021 tcagtggtaa ctttacctac tatgtggcgt ttgaaaagga tggcagaatt agccgtccca
     7081 gtcctattag cgatcctatc aacatcctca acggtcgagt ggttctcaag gatttaccc
//
LOCUS       DAOPTR010000042         7126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_8698_length_7126_cov_3.867628, whole genome shotgun sequence.
ACCESSION   DAOPTR010000042 DAOPTR010000000
VERSION     DAOPTR010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7126
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8698_length_7126_cov_3.867628"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PKI05_01510"
     CDS             complement(<1..366)
                     /locus_tag="PKI05_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOA50901.1"
                     /translation="MATLPIYLDNHATTPCDPVVFEAMRPYFLEHFGNAASTHSYGEA
                     ASAAVEESRAIIAGLLRIDPEEIIFTSGATESNNLAILGTARRLSSDSHFITTAIEHT
                     SVLEPFRKLEKEGRHVTILP"
     gene            complement(391..1026)
                     /locus_tag="PKI05_01515"
     CDS             complement(391..1026)
                     /locus_tag="PKI05_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HOA50902.1"
                     /translation="MASLDTNEKVLVIPTSLFRKLGYFQGFSGDIANYIDDISNPANT
                     MFRPRGEVEPDPQFKQLIPYVIICHNQGQTWQVFSYTRGTGQGESRLHRKRSIGIGGH
                     ISDRDLTEGGNIVFQAIERELNEEVAVNSSYRMECVGLINDDTTDVGRVHLGIVYRCI
                     VSSPAVVPREKEIVSHGFLPVWELVKHWNEFETWSQIALKGVFGVEPPSLA"
     gene            complement(1098..2144)
                     /gene="queA"
                     /locus_tag="PKI05_01520"
     CDS             complement(1098..2144)
                     /gene="queA"
                     /locus_tag="PKI05_01520"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOA50903.1"
                     /translation="MLDTDCYDYELPKHLIAQSPLPNRADARLMVVNRATGEIEHRHV
                     RDLPELLRPGDALVLNNTKVVPARLVGYRTATGGRWEGLFVEADASGVWHVIGKTRGK
                     LQPGESITLVNLQGRDDIRLFVIGRIAEGGWLVSPDTAEDSWEVLNRVGRVPLPPYIR
                     GGQMLEEDRNRYQTVYAEVPGSVAAPTAGLHFTQGLLRKLKEAGIEICYLTLHVGLGT
                     FVPIKTSRIEEHRMHAEWGTISEEVVERLKQIRSSGGRIVAVGTTVTRLLETAALDGE
                     LKAFSGRTDLFIRPPYQFRAVDVLMTNFHLPRTTLLVLVRTFGGDNLIKRAYEEAIRE
                     SYRFYSYGDAMLIF"
     gene            2795..3871
                     /gene="prfB"
                     /locus_tag="PKI05_01525"
                     /pseudo
     CDS             2795..3871
                     /gene="prfB"
                     /locus_tag="PKI05_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            3992..4393
                     /locus_tag="PKI05_01530"
     CDS             3992..4393
                     /locus_tag="PKI05_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50904.1"
                     /translation="MDVERLRSLRFEYENQFVIVEGDRPELLRFKGKIGQVKTVNCNG
                     RALVQFDSGADRGWYDIDLDFLRVVDPPVNKGDSAESPASPDRLPQEVDYRPSALELA
                     RQAKGQGSSVEKNPLRDVRSQGTVGKKDESR"
     gene            4461..4784
                     /gene="cutA"
                     /locus_tag="PKI05_01535"
     CDS             4461..4784
                     /gene="cutA"
                     /locus_tag="PKI05_01535"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOA50905.1"
                     /translation="MKQEALQVMVTFPSRESAESVAKGLVENRLAACVQILGPIDSIY
                     RWQGQIESAKEWLCLLKTTREAFEKLADFVRKQHPYEVPEIIGLPICQGFPPYMQWLF
                     QSIEG"
     gene            complement(4859..6289)
                     /gene="dnaB"
                     /locus_tag="PKI05_01540"
     CDS             complement(4859..6289)
                     /gene="dnaB"
                     /locus_tag="PKI05_01540"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011118493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOA50906.1"
                     /translation="MAGDDSRRTQDNQLSQLSDLVGRVPPHSVEAEKAVLGSILLDPR
                     VLDDVATQLTADDFYVDAHRRIYARILEMHSENRTIDLMLLVEELRRHSEFEAVGGAA
                     YLAEIAQSVAVASHAVYYAEIVRNHALTRSLIHAGTDIVRDAFDLSLEPREIINRAEE
                     RIFAVRDRRSSGDAVPVNDVLFEAFQMIDRRMQGEATGVPTGFTDLDKLTGGLHDSEL
                     IILAARPSMGKTALATNIAEHVSVDSGLTTLFVSLEMSRLELVQRMLCSRGKINGHKF
                     RSGFLSAEDREKLVRAAGELSRGQLLIDDSPSKTVTEIAAVARRLKRRAGLRLIVIDY
                     LQLIEPDNPKDPRQEQVAKITRRLKTLARELKVPILCLAQLNRQTEVAKDNRPRLSHL
                     RESGAIEQDADVVMFVHREEYYHTKEEAQARGLCGLAEVIVAKQRNGPIGDVKLTWRE
                     EYTRFENAAAGDFAEVDYVPYEDSPF"
     gene            complement(6570..>7126)
                     /locus_tag="PKI05_01545"
     CDS             complement(6570..>7126)
                     /locus_tag="PKI05_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOA50907.1"
                     /translation="QKRNEFIQLVRESVAQKKTVVILDSGDPLIYGPWAWCLEEFEDL
                     KPVVVPGLSSFNAANAALRRGVTNASKTKSVILTANDWPGKTDTIEKLSSLQATMVIF
                     TMRAEFDYFIRQLRTHYPPETPIAVVKYAGYVDREEVVQSTLGRVYEQIDPAALPFEY
                     LIYVGDFLTYRHKSSQPVTSPARE"
BASE COUNT         1620 a         1911 c         2003 g         1592 t
ORIGIN      
        1 cggaaggatt gtgacgtggc gtccctcttt ttcgagcttt cgaaatggct ccagcaccga
       61 ggtgtgttcg atggccgtcg tgatgaaatg cgaatcgctg gaaagtcgcc gtgccgtgcc
      121 caagatcgca aggttattac tttccgtagc accgctggtg aagatgattt cctcagggtc
      181 gatacggagc aggccggcaa tgatggcccg gctttcttcg acggcggcag aggcggcctc
      241 gccataggaa tgcgtcgatg cggcattgcc aaaatgctcg agaaaatagg gtcgcattgc
      301 ctcaaacacc accggatcgc atggtgttgt ggcatgattg tccagataga tgggtaaagt
      361 cgccatgttc accggatcaa agcggggaat tcacgcaagt gaaggtggtt caactccaaa
      421 aacacccttg agggcaatct gggaccacgt ctcgaactca ttccagtgtt tgaccagctc
      481 ccacacgggc agaaaaccgt gactgacgat ctccttttcc cgcggcacca cggctgggct
      541 ggacacgata caccgataaa cgatccccaa atggacgcgg ccaacatctg tcgtgtcgtc
      601 gttgattaat ccgacacatt ccatccgata actgctgtta acggcaacct cctcgttcag
      661 ttcccgttcg atggcctgaa agacgatgtt ccctccctcc gtcaaatctc ggtcagagat
      721 atgtccacca attccaatac tccgcttgcg atggagtcgg gattccccct ggcccgtgcc
      781 ccgcgtatag gagaacacct gccaggtctg cccctggttg tggcaaatga tgacgtaagg
      841 gataagctgt ttgaactgcg gatcaggttc aacttccccc cgcggacgga acatggtgtt
      901 tgctggattg gagatgtcgt caatgtagtt ggcgatatct ccgctaaacc cctggaagta
      961 gccaagtttg cggaatagcg atgtgggtat gacgaggacc ttctcgttcg tgtccagtga
     1021 agccatagat atctctttcc tttccggtat ccaaatcgtt ggagatccag tttcgacttc
     1081 ccatgcaaca accctcttca aaagatgagc atggcatctc cgtaactgta aaatcggtaa
     1141 ctttcgcgga ttgcctcctc atacgcccgc ttgataaggt tatctccccc gaatgtcctg
     1201 accaggacaa gtagggttgt ccgcggaaga tggaaattgg tcattaggac gtcaaccgct
     1261 cgaaattgat acggcggtcg gataaatagg tccgttcttc ctgaaaatgc ctttaattcg
     1321 ccatcgaggg ccgccgtttc cagaagccgc gttaccgttg tgcctacggc aacgatccga
     1381 cccccagagc tgcgaatttg ttttagccgt tccaccactt cctccgaaat tgtcccccat
     1441 tcagcatgca tccgatgttc ctcaatacgg gaggtcttga tcggtacgaa ggtccccagc
     1501 cccacgtgaa gggtgagata acagatttcg attcctgcct cttttagctt tcgcaacaat
     1561 ccttgtgtga aatgcaggcc ggcggttggt gcagccactg agccagggac ctcggcataa
     1621 acggtctgat aacggttgcg gtcctcttct agcatttggc caccgcgaat atagggtgga
     1681 agtggcacgc gccctactcg gttgaggacc tcccatgagt cctcggcggt gtcagggcta
     1741 acaagccacc caccctcggc gatccgtccg atcacaaaaa gtcggatgtc atcacgtccc
     1801 tgtaaattga ccaacgtgat gctctcgcct ggctgaagct tgccgcgggt cttgccgatg
     1861 acgtgccaca ctccgctggc atcggcctcg acaaaaagac cctcccaccg accgcccgtg
     1921 gccgtccgat agcccaccag cctcgccgga accactttgg tattgtttag gaccagggca
     1981 tcccccggcc taagaagttc cggcagatcg cgcacatgcc ggtgctcaat ttcgcctgtg
     2041 gcgcggttca ccaccatcag ccgggcatcg gcacggttag gtagcggact ttgggcgatc
     2101 aagtgtttag gaagctcgta atcgtaacaa tccgtgtcaa gcatttgtca ataactttaa
     2161 tgttgtattt ggagaacgaa atttaagcta acccgcctag gtgccctcaa caactttcct
     2221 ccaattacca agatctgtcc aaaggggcgc tttaggcacg actattgcca cccaagccgc
     2281 cggcggaccg tatcgtctta tgattctacg ccactccctt tgccctgggt atgacatggt
     2341 ctggcggtgc gttcatcttg gtgaagcgtg cttcgccgtg ggcggaaaac aattcgggag
     2401 ggtcatccga agtcttggag cagattagcc ttataggccc gtgcgctggg cggctgaggt
     2461 acctgatgat ggaatgccga ttagtacttc ggcggacgcg acggcatgtg tcttgcaaag
     2521 gcccacgaga ttgcgtggcc gaaaatccgg cgtcgttcca gtgatggtgc tctattcccc
     2581 gtgagcggct gtcacgtcag cggcttctgg gtgcgttcag atagggctgg gaaacggggc
     2641 cgaccattcg ttaaaatgac attaggtgag ttggaccatt aacgcgaagc tgccctccag
     2701 ttggggttga tcgacagagg agattgtatg gtcgtggaac gcgaagtcat taatcgtgcg
     2761 gaatctattc gtgaacgcct cacgcagtta agggactccc tttgactacg ccagcaaaaa
     2821 ggcgcgggag tccgagattg agcaaattat ggcccagccc agcttctggg acgacattga
     2881 aaagtcgcaa gagatggtgg ctgagctgaa gtcgattcgc gcgattctca agcctcttga
     2941 ggaagccatc aagcagctcg aagacatcga cgcgatggtc gaacttgccg aagaggagcc
     3001 ttcactagca gacggggttc ccgacgcgct gaacaaactg gaaaaacttg tcgaagatct
     3061 cgaaatcaag gcgcttctca acgggccgca cgacaactgc aacgccattg ttagtatcaa
     3121 tgcccgcgat ggggggaccg acgcccatga ttgggcggag atgctgctcc gcatgtacat
     3181 caattgggcg aaggcccacg attatgaggt cgaggttttg gaccgcattg acaatgatgt
     3241 ggccggtatc aacagtgcta cgattgcaat tcgcggcccg atggcctacg gctaccttaa
     3301 aggcgagacg ggcatccatc gtttgatccg aattagcccc ttcaatgcgg agggcaagcg
     3361 acagaccagc ttcgcggcgg tcgatgtttc gccggagatt aaaaatgagg ctgagatcga
     3421 aatccgggaa gaagatatcc gcgaggatgt ctttcgtgcc agcggccccg gcggacagca
     3481 cgtcaacaag acgtcaagtg ccgttcgcct gacgcacctt cccacgggaa tcgtggtggc
     3541 ctgccaaagt gaacggagtc agcatcaaaa tcgggccatg gcgatgagga tgctccgcgc
     3601 gaaactcgct cgtcttgagg aagagaagcg agaggccgaa atggcggcta agtacgaagc
     3661 cgccccgaag gtgggtttcg gttcgcagat tcgcagctat ttccaacatc cggagcagcg
     3721 gatcaaagac gcgcgaacgg gatacacgga gacgagcttc caatcggtga tggatggcga
     3781 aatccaagga ttcttggatg cctatcttcg ttggaaagtc gccgaagaca ctgcccggat
     3841 gaaagctgct ggcggcgtca gtaagaacta aggacctcgg tctctgttcg cccttttgac
     3901 cacccatgaa aggtgagctt cttgaatgcg atgggacgca ataatcattc ttatgcttcg
     3961 atgagataac aggctggcgg agttgttggc catggatgtt gaacgcttgc ggagtctccg
     4021 gtttgagtac gagaaccagt ttgtcatcgt cgaaggggat cgaccggaac tgttgcgatt
     4081 caaagggaaa atagggcagg tgaagacagt gaactgtaac ggccgagcac tcgtgcaatt
     4141 cgactcgggt gcagaccgcg gatggtacga cattgacctc gacttcctgc gagtggttga
     4201 tccgcccgtc aataaagggg attctgcaga aagccccgcc tctcccgacc gactgcccca
     4261 ggaggtggat taccggcctt cggcgctgga gttggcaaga caggccaagg ggcaaggctc
     4321 atcggttgaa aagaatccgc tgcgtgacgt acgctcgcag gggaccgttg gcaaaaagga
     4381 cgaatctcgg tgacaatttc tcagggttga gagcaagagt tgtgccgttt caagcttgac
     4441 cacggcaagg ggaacggagg atgaagcaag aagcccttca agtgatggtc acgtttccca
     4501 gcagggaaag tgccgaaagt gtggcgaaag gtctggtgga gaaccgcttg gccgcgtgcg
     4561 tgcaaatact cggtcctatc gacagtattt atcgatggca ggggcagatt gagtcggcca
     4621 aggagtggct gtgtctcctt aaaaccactc gcgaggcctt cgaaaagtta gcagatttcg
     4681 tccggaaaca acacccgtat gaggtccctg agataattgg ccttccgata tgccaaggct
     4741 ttccgccata tatgcagtgg ttattccaat caattgaggg ttaatgtggg cacttggaac
     4801 tcggtccaaa caactgcaat acgagcaaag cgaaagtaat tttgcgcggg gtgggccgct
     4861 agaacggtga gtcctcgtac gggacgtaat cgacttcggc aaagtccccg gccgctgcat
     4921 tttcgaaccg ggtatattct tctcgccagg tcaatttaac atcgccgatt ggcccgttgc
     4981 gctgtttggc cacgatgacc tctgccaggc cacacagccc tcgtgcctgt gcctcttctt
     5041 tcgtgtggta gtattcctca cggtgaacga acataacaac gtccgcatcc tgctcgatgg
     5101 caccggattc tcgaaggtgg ctgagccgtg ggcggttgtc cttggccacc tctgtttggc
     5161 gattcaactg ggccaagcaa agaatgggga ctttcaattc cctggcaagt gtcttaagcc
     5221 gccgcgtaat cttggcgacc tgctcttgtc gcgggtcttt ggggttgtca ggttcgatga
     5281 gttgcaagta gtcaatgacg atcaatcgga ggcctgcgcg gcgttttaaa cgtcgtgcca
     5341 cggccgcaat ttctgtaacg gtcttgctgg gggaatcgtc aatcaatagt tggccacggc
     5401 tcagttcccc tgccgctctt acgagttttt cccgatcttc cgcggagaga aaaccactgc
     5461 ggaacttatg accgttgatc ttcccccgcg agcaaagcat ccgttgaaca agctcaagac
     5521 gcgacatttc aaggctgaca aacagcgttg tcaacccgga atccacggag acatgctccg
     5581 caatattcgt cgccagcgct gtctttccca tgctcggtcg ggcggcgagg atgatcagtt
     5641 ccgaatcgtg caacccgccc gtcaacttgt cgagatcagt gaaccctgtt ggcacccccg
     5701 tcgcttcgcc ctgcattctg cgatcgatca tctgaaacgc ttcaaacagg acgtcgttga
     5761 cgggaacggc atccccggat gaacggcgat cgcgaacggc gaaaatccgt tcttctgccc
     5821 gattgatgat ttcccgcggc tcgaggctga ggtcaaaggc gtcacgaacg atatcggttc
     5881 ccgcatgaat cagcgagcgg gttaaggcgt gattgcggac gatctcggcg taatagacgg
     5941 catggcttgc cacggccacg gactgggcaa tttcggcaag ataggcagct ccacccaccg
     6001 cctcgaactc gctgtgtcgg cgaagttctt ccaccaagag catcaaatcg atcgtgcggt
     6061 tctccgagtg catctcgaga atccgcgcgt agattcgccg atgggcgtcc acgtagaaat
     6121 cgtccgccgt tagttgggtc gccacgtcat ccaagacccg cggatccagg aggatacttc
     6181 ccagcaccgc tttctcagct tcaacactat gtggaggtac acgaccgacg agatcggaaa
     6241 gctgggaaag ttggttgtct tgagtgcgcc gcgaatcgtc tcctgccaaa gctaataacc
     6301 tccttttcag cgggtggatc agagaattga ccagcgactg agaccaaccc agctaatcgc
     6361 ccgacttcat ggttccttct agaagaccaa tctagcgcgc ggcgttgaaa gcaacaagtt
     6421 ccccaaattg gggacaggtg accatcttcc gaagggcatc tgtcaacact acggtcgcag
     6481 attggtccca cgatgcgtgg acatttggtg tcaaaggatg gacgctcggt actgcccggg
     6541 cgaaatggca acgagtgcgg tcgaataggt tactctctgg ctgggctcgt gacgggttgg
     6601 gacgacttgt ggcggtacgt caggaagtcg cccacataaa tcaaatactc aaacggtagg
     6661 gctgcgggat cgatctgctc ataaactcga ccgagtgtcg attggacgac ctcttcgcgg
     6721 tccacgtagc ctgcgtattt gaccaccgcg ataggagtct cgggaggata gtgcgttcga
     6781 agctgtcgaa tgaaatagtc gaattcagcc cgcatggtaa agatcaccat agttgcctgg
     6841 agggacgaga gcttttcgat ggtatccgtt ttgcccggcc aatcattcgc ggtcaggatc
     6901 accgactttg ttttcgaagc attggtaacc cctcgacgga gtgcggcatt ggcagcatta
     6961 aagctgctta ggcctggcac gacgacgggc ttcaaatcct cgaactcctc aaggcaccat
     7021 gcccaaggac cgtagatcaa gggatcccca ctatcgagaa tgaccaccgt tttcttctgc
     7081 gcgacgctct ctcgtactaa ttggatgaat tcatttcgtt tttgag
//
LOCUS       DAOPTR010000043         7108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_8733_length_7108_cov_3.906706, whole genome shotgun sequence.
ACCESSION   DAOPTR010000043 DAOPTR010000000
VERSION     DAOPTR010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7108
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8733_length_7108_cov_3.906706"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            397..1896
                     /locus_tag="PKI05_01550"
     CDS             397..1896
                     /locus_tag="PKI05_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50908.1"
                     /translation="MCESCGLGRFPRRDVLQMAGLAGVSAMVNTLVARASQDSSAVAQ
                     EVVLPPIEKKPPRIWAVFLYPPADVVLAGKMEDSWAPHQWFTWPGNQFEPEAQQAKFT
                     GVIQKMASDLGIDLVIHPEALYQKAKVDEFIAQARAAELDAVLVINFWNTLSWAAARI
                     ATEAAPTAIVYHSLGSNHQLPPESLRKAEGLYYIHSMENFAEIERGLRAVRAKKMLAQ
                     SRVLRVSGAVKELTESQEPSLGTAIVAIPAAEFNDLFDSISPTPQLQEEAAAFRRMAS
                     KVVDVSEEAMVDAIRAHHAVQHIMRRYGADAITIQCLMLKHRKPCYSFAWHNGQLVPC
                     GCENDINATLTLMLGRWLLDKGGFQHNPEFDTSENLYFGAHCTCAWKLHGPQGPSQEF
                     FVRPFFHQLPKTPALDVQWSPGEPVLLTKYLTGRNTLMCYRGKVISSPTCPPTGGCAT
                     RVLVELEGVADVCEAYAGPHPVLFCGDRGDARCFKAFAKMYRFTLLGNA"
     gene            2291..4738
                     /locus_tag="PKI05_01555"
     CDS             2291..4738
                     /locus_tag="PKI05_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOA50909.1"
                     /translation="MSEPEPSPNTAAESAQAAPVEQGGRVSLRTRLVAMATSVVKSKF
                     RLALAVGIGLTLIAVGAGVGLWFFLRGSPDNSQAEQILSALKRGDYPLVRELTDKVLA
                     TEDVSPDQLRVALSARFFAVAREADAAKGEDRRRLLDVAINYGEQAASVGFTRGWEEE
                     AAFLLGRCLHYRARFAESRKWLREAGKRTVDKQARVYWLLAESHLLDPQGIKEEALKF
                     NEAFLQISRLDVQAKNAGWMQRAKIQIALGNPDDATSSLTKLSPQAAQSPEAQFLRGW
                     IDYELGKRLLTSQEENKAGQDRLRSAAERLERVRRLVDADESLRSRATYLLGLVFEGL
                     GEYDKAIAAWEGCHSEYPGTYEAWAARLRLAQLYQETSRYQQASEAFISCFREIVDSQ
                     EFFNPWVKWSEAQTLVRDALATYKEKQLYEIAMNVIDSLEKSFAEAEVHELRATVLSA
                     WGETLLRQAQSESSDRQINITWAKRCFRLAGDEFTRLARTRSGTRFYTEDLWQAAEHY
                     YRGGSYSRASRVYREYLRNEARRRNPIALLRLGECLLALGRVDEALLVLNECIEFHRK
                     DAASYSARLLAAHAHMEKGSLDKAEEMLLANLSGELAPTSVEWRDSLFTLGEMYYQLG
                     RDQDAIPRLEEAIRRYPEDPRSTKAKYYLAEIYSRLAWNRMMLSPSSSAATPDMATIR
                     NDFLRSLHYYRQVQSDLDFQWTTEEPSEDTRKMLRNTYFAIGRVCYHLGQFAEAVAAF
                     TTITNRFQAEPEVLDAYVEIARCRRAMGQNPDARIALEQAKIVLTRFPNEIPFERTTP
                     FSRAEWATRLDKLLQTP"
     gene            4922..5371
                     /gene="flgN"
                     /locus_tag="PKI05_01560"
     CDS             4922..5371
                     /gene="flgN"
                     /locus_tag="PKI05_01560"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HOA50910.1"
                     /translation="MPHSANVYESLADLIVQKHRCLKRVHELGQRQMGCVTSGDIEGL
                     LEILAVKQRVLGDLQTIDHRLKEIGAYGDEVDAIPQEWREAIAGVLIESRALLEEILR
                     TESECAHILEQRKQDIAKQLAELHDFVRARTAYQQPGDESGQLDLTL"
     gene            5446..6300
                     /locus_tag="PKI05_01565"
     CDS             5446..6300
                     /locus_tag="PKI05_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA50911.1"
                     /translation="MNQKTDLELSAETDLGTILEAWHAATIRLEQTHEALQAEVRRLT
                     AELERKNRELARKDRLADLGRMAAHIAHELRNSLVPVALYLSLLERRTEDEGSREILH
                     KVIEAVQAAETTVTDLLQFAADREPQSVLFPLLGLVEEVLAGLEPQIQAQQIKTVVEV
                     SQALYVWADRELMRRVLLNLVLNALDAMPHGGTLTIEAHAEEGDLWLRVSDSGSGLDE
                     EHLHRIFEPFYTTKPNGTGLGLAIVECLTELHGGHVQASNNPEGGAQFVVVLPQRAVL
                     SGHQQEGC"
     gene            6356..>7108
                     /locus_tag="PKI05_01570"
     CDS             6356..>7108
                     /locus_tag="PKI05_01570"
                     /inference="COORDINATES: protein motif:HMM:NF039486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOA50912.1"
                     /translation="MTRDKRTELNYRKYDTQPGTVLVVDDHTPSRQAIADILRQAGHH
                     VEMCSSAAEAMSALNRADYDVVVTDLNMPGMSGLDLVRYVEKQQLRTRPIMVTAYATV
                     SSAVEAIRHGAFDYIEKPFDANQLERLVAEAVRHSRLRLAGQANRTGDHTIKAVNLVG
                     SSSLMTELRQRIAQVAGTSETVLIYGESGTGKELVARAIHVQSSRAAGPFVSVNCPAL
                     SAHLMESELFGHEKGAFTGADCDRVGRFEMANG"
BASE COUNT         1600 a         1856 c         2087 g         1565 t
ORIGIN      
        1 cgctcccagt tcaccgccct attgtatcaa gaaccacaca gtacgacatg cagtgaccga
       61 ctcaaaatgc catgagcgcg catcgtgaga tgcggtattt gctgatcgac tgtgcgttct
      121 cccgccgtcc ttagcggtag ggcttgtgcc ggcccgacat tataaagggt cacataccgc
      181 cgtgatcacg gcaaactcag gtagcgaagc cgtatgaggt gaaaggaagt cacatcgcaa
      241 acggttccct ggtgttccag tcgctttgct gaggtcgaag gttgtttgcc cccaaccccc
      301 acggtatgat gatagcacgt tttattgggg ggagttctgg ccgattgaag gtgtagggaa
      361 aggccgaatt gcaatggtca aataggaggc caaagtatgt gtgaaagttg tgggttgggt
      421 cgttttcccc gacgcgatgt tctccagatg gcgggcctcg caggggtttc agcaatggtc
      481 aacacgttgg tggcacgtgc ctctcaggat tcttcggcgg tggctcagga agtcgttctt
      541 ccacccatcg aaaagaaacc gccacggatc tgggcggtgt tcctatatcc gccggccgat
      601 gttgtgcttg cgggcaagat ggaggactct tgggccccac accaatggtt cacttggccg
      661 gggaatcagt ttgagccgga agcgcagcag gctaagttca cgggggtcat tcaaaaaatg
      721 gccagcgatt tgggtatcga tctcgtcatt catcccgagg ccctatacca gaaggccaag
      781 gtggacgagt tcattgccca ggcccgtgcc gcggagcttg acgccgtgct ggttatcaat
      841 ttttggaata cgctgtcctg ggcggcagca cgaattgcta cggaggctgc gccaaccgca
      901 atcgtgtatc attccttggg atcaaatcac cagcttccgc cggaatctct tcgcaaggcg
      961 gaaggtctct attacatcca ttccatggaa aactttgccg agattgagcg aggattgcgg
     1021 gctgttcgcg cgaagaaaat gctggctcaa agcagagtcc ttcgcgttag cggggctgtc
     1081 aaggagttga ccgaaagcca ggagcccagt ctcggtacgg ccatcgtggc aatcccggct
     1141 gctgagttca acgatctgtt tgactcgatt tctccaactc cacaacttca ggaggaagcc
     1201 gctgcctttc ggcgaatggc aagcaaagtt gtggacgttt cagaggaagc gatggtggac
     1261 gcgatccgtg cccatcacgc tgtgcagcac atcatgcgtc ggtatggcgc tgatgccatc
     1321 accattcaat gcctgatgct gaagcaccgc aagccttgtt acagttttgc ctggcacaat
     1381 gggcaactgg tgccctgcgg ctgtgaaaac gatatcaatg ccacgctcac cctcatgctg
     1441 gggcgatggc tccttgacaa aggcggtttc cagcataatc ccgagttcga caccagtgaa
     1501 aatctctatt tcggggcaca ctgcacctgt gcgtggaaac ttcatgggcc acagggccca
     1561 tcgcaggagt tttttgtccg cccgtttttc catcaacttc cgaaaacccc ggccttggac
     1621 gtccagtggt cgccgggcga accggttctt ctcacaaaat atttgacggg ccgaaacacg
     1681 ttaatgtgtt atcggggaaa agtcatcagt tcgccaacat gccccccaac cggcgggtgc
     1741 gccacgcgag ttctggtcga actggagggg gttgccgacg tttgcgaagc ctacgccggt
     1801 ccgcatcccg ttctcttttg tggtgaccgc ggcgacgcta ggtgttttaa agcattcgcc
     1861 aagatgtatc gatttaccct tttggggaat gcctgagcct ctgagaggcg gttgacgcaa
     1921 atcggaatag gcccgccgac atggctatgc gctcggtcgg tggtacccca ctgaggacct
     1981 gcaaaagtag ctagattcct taaagtcgcc atcttgtcgc attgtgtggc ggtgacacat
     2041 ctgccatttc gttggccgaa tcgccgaaat tgccttcttt ttcagtcttc agcagattga
     2101 cacgtcttac cgtttgatgt tgacgggcga cggttcattc tgtacatact ttctccccgg
     2161 atatatgcca tcagtgcggg taaacctgca aagaatgcag cgtttgtgtc tgggacggta
     2221 gtgatctgtg gaagcgatag cctttacttg gtcggcggaa gcctttgtag gagacgacaa
     2281 ttcatgaccc atgtccgaac ctgaaccctc ccccaacaca gctgccgaaa gtgcccaggc
     2341 cgcccccgtt gaacagggcg gcagggtatc actgcggact cggcttgtgg ccatggcaac
     2401 gagcgtggtt aagtccaagt ttcgcttggc cctggcagtg gggattgggc taaccttgat
     2461 tgcggtcgga gcgggggttg gtctgtggtt ttttctgcga ggttcaccag ataactccca
     2521 ggccgagcaa atcctttcgg ctttgaaaag gggtgattac cccttggtcc gagagcttac
     2581 tgataaagtc cttgccactg aggacgtgtc gcctgatcaa ctacgggtgg ccctgtcagc
     2641 acggtttttt gccgtagcga gagaggccga cgcggctaag ggcgaagacc gacgtcgcct
     2701 cttggatgtg gccattaatt atggagagca ggcggcatcg gttggcttta cccgcgggtg
     2761 ggaagaggag gcggcatttc ttttgggacg gtgccttcat taccgcgccc gatttgcaga
     2821 aagccgaaaa tggctgcgcg aggctggtaa acgcacggtg gataagcagg cccgagtcta
     2881 ttggcttctc gccgagtctc atctactaga tccgcaagga attaaggagg aagcccttaa
     2941 gttcaatgag gcattcttgc aaatttcgcg tcttgatgtg caagcaaaga atgccgggtg
     3001 gatgcagcgg gcgaagattc agattgccct gggaaatccg gatgatgcca cgtcgtcgct
     3061 gacgaagctc agcccacaag cggcgcaatc acctgaggcc caatttcttc gcggttggat
     3121 cgactacgag ttaggaaaga ggcttcttac aagccaggaa gaaaacaaag ctggccagga
     3181 cagattgcgg tcggctgcgg agaggcttga gcgcgtgcgg aggttggtcg atgccgacga
     3241 gtcgctccgc tcgagggcta cttatttgtt agggctggtt ttcgagggcc ttggtgaata
     3301 cgacaaggcc attgcggctt gggaaggatg tcactccgag tatccgggga cttacgaagc
     3361 ttgggccgcc cgcctgcgcc tggcccagtt ataccaggag acgtcacggt accagcaggc
     3421 cagtgaagcc ttcatctctt gttttaggga gatcgttgat tcacaggaat tctttaaccc
     3481 gtgggtaaag tggtctgaag cccagaccct cgtgagagat gcccttgcaa cctacaagga
     3541 gaagcaactc tacgagattg cgatgaacgt gatcgactcc ttggagaagt cgttcgccga
     3601 ggccgaggtg cacgagttgc gggccaccgt cttatcagcg tggggtgaga cgctcctccg
     3661 acaagctcaa agcgaatcgt ccgaccggca aatcaacata acgtgggcca aacgatgttt
     3721 tcggctggcc ggggacgagt tcacacgact ggcacgcact cgaagtggga cgcggtttta
     3781 cacagaggat ctgtggcagg ccgccgagca ttactatcgt ggggggagtt actcacgggc
     3841 atcgagggtt taccgagagt atcttcgcaa cgaggcccgg cgccgtaacc caattgcctt
     3901 gttgcgactg ggcgagtgcc tactggcttt ggggcgggtc gatgaagcac tactcgtcct
     3961 taatgagtgt atcgaatttc atcgaaaaga tgcggcctcc tattctgcgc gacttttggc
     4021 cgcacacgca catatggaaa aggggtcgct cgataaagcc gaggagatgc tcctggcaaa
     4081 cctttctggt gagctggcac ccacgagtgt cgaatggcga gattcgttat tcacccttgg
     4141 tgagatgtac tatcagctcg ggcgtgacca ggacgcgatc ccccgcttgg aggaagcaat
     4201 tcggcggtac cctgaggacc cccgcagcac caaggccaag tattaccttg ctgagattta
     4261 ctctcgactg gcatggaatc gaatgatgct gtctccctcc agtagcgctg ccactcccga
     4321 catggccacg atccgcaacg atttcttgcg gtcgctccac tactatcggc aagtccagag
     4381 tgatttggac tttcaatgga caacggagga gccaagcgaa gacacccgca aaatgctccg
     4441 caacacgtac ttcgctatcg gtagggtgtg ttatcaccta ggtcaattcg ctgaggccgt
     4501 ggcggccttc acgaccatta ccaatcgatt tcaagcggag ccggaggtcc tggatgccta
     4561 tgtcgagatc gctcgctgtc gtcgagctat gggacagaat ccagatgcac gaattgccct
     4621 ggaacaggcg aaaatcgtcc tcactcgttt tcccaatgag attccatttg aacgaacaac
     4681 tcctttttca cgcgcggaat gggccacgcg tctggacaaa ctgttgcaaa caccatgatc
     4741 agttcaaaag tcaaccttga gaggctttct caaaaagtcc cctctcccgc cgaacgggcg
     4801 aggggagatt atggaatagg ctctaagttg ctcctggcag ttgagcagcg gaagccgttg
     4861 aaagcaggaa tgaatttacg gagaagacat tgatcaaagg cctacagaac gtgtgaactt
     4921 tatgcctcac tctgcgaatg tttatgagtc cctagcggat ctcattgttc agaagcaccg
     4981 gtgtttgaaa cgggtacacg agctgggtca gcgccagatg gggtgcgtca catcgggcga
     5041 tatcgagggt ctccttgaga tacttgcggt caagcagcgt gtcctcgggg atttgcagac
     5101 cattgatcat cgccttaaag aaattggcgc ctatggagac gaagttgacg ccattccaca
     5161 ggaatggcga gaagccattg ccggtgtgct gattgaaagc cgggccttat tggaggaaat
     5221 ccttcgcaca gaaagtgaat gcgcccacat tctggaacaa cgcaagcagg acattgccaa
     5281 acagttggcc gagcttcatg actttgtgcg ggcccgcacg gcatatcagc aacccggtga
     5341 tgagtcgggg caactggatc tcacattgtg aatccatcgg gcagagttgt tcgaaaacca
     5401 aacggaagtg gtaacaatcg gcaaacgatc atcggaggat cttctgtgaa tcagaaaacg
     5461 gatttggaac taagtgccga aactgatctg ggcacgatcc ttgaggcatg gcatgcggcc
     5521 acgatccgcc ttgagcaaac ccacgaggcc cttcaggcag aagtccgccg attgaccgcg
     5581 gaactggagc gcaaaaatcg tgaattggcg cggaaggacc gcttggcaga cctaggtcgt
     5641 atggcggccc acattgccca cgagcttcgc aacagtttgg tccccgtggc cctatatctg
     5701 agccttttgg agcgccggac tgaggatgag ggatcccgcg agatcctcca caaagtcatt
     5761 gaggcggttc aagcggcaga aaccacagtg accgacttac ttcagtttgc agcggatcga
     5821 gaaccgcaga gtgttctatt tcccttgcta gggctcgtcg aagaggtgct tgctgggctt
     5881 gagcctcaaa tccaggcaca gcagatcaaa acggtcgtgg aggtttctca agccctctat
     5941 gtgtgggcag atcgcgagct aatgcgccgg gtcctcctca atctggtgct caacgccctc
     6001 gacgccatgc cccacggggg gaccctcacg atcgaggctc atgcagaaga gggtgacttg
     6061 tggctgaggg tcagcgactc gggttcgggg ttggacgagg agcacctgca ccgaattttt
     6121 gaacctttct atacgacgaa gcccaatggt acgggtctag gattggccat tgtggaatgt
     6181 ttgacggaac ttcatggcgg acacgtgcaa gcaagcaata acccagaagg cggcgcacaa
     6241 tttgtggtcg tgttacccca gcgagccgta ttgtccggcc accagcagga gggatgttag
     6301 gatcatcgtt tccggataac tttgaagcat ttccgggagt ttaatagaga ggcccatgac
     6361 acgagacaaa cgaactgaac tgaattatcg caaatacgac acccagccgg gaacggtgtt
     6421 ggtggtggat gaccacacac cgtctcggca ggcaatcgcc gatattcttc ggcaagccgg
     6481 ccatcatgtg gaaatgtgtt ccagtgcagc cgaggcgatg agtgccctca atcgcgctga
     6541 ctatgacgtg gtggtgacag accttaatat gccgggcatg tcggggctgg atctggtccg
     6601 ctacgtggag aaacaacaac tgcggacccg tcccatcatg gtcacggcgt atgccaccgt
     6661 ttcctccgct gttgaggcca tacgccacgg cgccttcgac tatatcgaaa aaccatttga
     6721 tgccaatcaa ctggagcgcc tggttgcaga agcagtacgc cacagcagac ttcgactcgc
     6781 tgggcaagcc aacagaacgg gggaccacac aatcaaagcc gtgaacttgg tgggatccag
     6841 ttccctgatg acggagctgc gacagcgcat cgcccaggtt gccggcacga gtgagaccgt
     6901 cctcatctac ggtgaaagcg gcacgggaaa ggaactggtg gcgcgggcca ttcatgttca
     6961 gagttcacgg gcagcgggac cgtttgtcag tgtcaattgt ccggcgcttt cggcgcacct
     7021 tatggaaagc gaactgtttg gccacgagaa gggcgcgttt acgggtgcag attgcgaccg
     7081 ggtgggaagg ttcgagatgg ccaacggt
//
LOCUS       DAOPTR010000044         7081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_8781_length_7081_cov_3.474523, whole genome shotgun sequence.
ACCESSION   DAOPTR010000044 DAOPTR010000000
VERSION     DAOPTR010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7081
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8781_length_7081_cov_3.474523"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1367..4810
                     /locus_tag="PKI05_01575"
     CDS             1367..4810
                     /locus_tag="PKI05_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOA50913.1"
                     /translation="MPQIFDNVTLKLIEGLEKAFDKAVAASFCIGYLNLRGWDQVAGL
                     VETLQGGDEDHACRVLVGMYRPPDEEMRALASLRLEQEAIDGQVRARLISRIEQIFRD
                     QLTVGVPSASVEQALRGLAAQLRARKVFVKAFLQYPLHAKLYLVQRDDPVAPLVGFVG
                     SSNLTLAGLSRQGELNVDVVEQDAAKKLQAWFDERWGDELAVDLSDKLATLIETSWAR
                     SKLVRPYLVYLKIAYHLSEEAGEEESRFRAPQIFAEKGTPLLNFQEKAVSLAIHHLHR
                     RGGVLLGDVVGLGKTLMATAIARVLQEDDDSNTLVICPPKLLPMWEQYLQDYEITGKV
                     LSLGKCDELSHLRRYRLVIIDESHNLRNRKGKRYQAIQEYIERNDARVLLLTATPYNK
                     QFTDLSNQLRLFLDENEDLHIRPERFFQQWIKDGKTEADFIAQFGTSPSSLLAFEKSP
                     FPEDWRDLMRLYLVRRTRHFIMKNYAEFDETRRRYFVRLNGEPKYFPVREPRRVSFKI
                     DENDPSDQYARLFRDDVVQIIEQLVLPRYGMAQFLIENADRLANEDQKRILDNLNRAG
                     KRLIGFCRTNLFKRLESSGHSFLLSVNRHILRNLIALHALRNGLPIPIGTQDPAMLDT
                     AINDADDELIIGAENDDPTAVDAREIDAGPVLQTATLAAYQALAAKTYKAYATKFRTR
                     FDWLDPKFFQPELANVLENDAQALFTVLEQAGPWNPDADAKLTALVELLTHHHPKEKV
                     LIFTQFADTARYLHKQLAKRGITDLGVATSEIHDPVSLARRFSPSTNGGLRNGETELR
                     VLVGTDVLAEGLNLQDAHIVVNYDLPWAIIRLIQRAGRVDRIGQKHDTILVYSFLPAD
                     GVDRIINLRTRLFRRLQENQEVIGTDESFFGEDAATKLRDLYTEKAGTLDDDQTDDDI
                     DLVSLALQVWNSASPEDKKAALSLPANVFATRALPETADPSQHPPGVIAYLRFPHRED
                     TLVRVDEHGNLVSQSLSSIFRAAACAPDTPPLPRSESHHDLVARCVEIVTQAQISTFG
                     LLGPLRGVRRKLYERLDRYRKLQQEHPTSSSDEILERLDTLIDLIYRYPLKRAAQDPI
                     SRQIRLGITDDALLELVWRHAEDKTLCEITEEDEASLSEPQIICSIGLVNDQWGENSH
                     E"
     gene            complement(5145..5369)
                     /locus_tag="PKI05_01580"
     CDS             complement(5145..5369)
                     /locus_tag="PKI05_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50914.1"
                     /translation="MQVGKNCFVGFKELACDGFDAEGPTKLVGDGGDGRLTGGAEFIQ
                     CECDFGEAFDGGPGWRTDFVGKCAELRLAV"
     gene            5364..>7081
                     /locus_tag="PKI05_01585"
     CDS             5364..>7081
                     /locus_tag="PKI05_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50915.1"
                     /translation="MQQRLYRLSDDKVWSHDYALQLLNRLMFLYFIQRKQWLGGDPRF
                     LANFWRAYKTSGQPKDTFFEQWLSVLFFEAFNNRFHGGHRQFPDHIRSALAQAPYLNG
                     GLFTRNSLDNAYHIKVPDEFFATLFDRFNGSTPGFLERYNFTITESTPLDIEVAVDPE
                     MIGKVYESLVNITFEGLTEEDQRGSAGIFYTPRVEIDLMCRLSLVDALANKLGESRKP
                     MLYDAVFAYDAIDKDKADQALAAQNLWPELNRLLREITVCDPACGSGSFLVGMLLVLD
                     DLQARANAQLGIEETPYERRRRIIGEQLYGVDVMDWAVHVAELRLWLQLVVETELKPA
                     ELKFRPLLPNLSFKIRCGDSLVQEIAGVNFGLHYSDPDVPAPLKGRITQLKGKKLRFY
                     QGETNLSETELKAEELAIFRDILKHKRDALQQEIDRLTRQIESRAEQGEFAAFSTGRP
                     KQERLNFQKQWRAERDEKQAELKRIEKNLEALCPTKPVPFVWDIAFVEIFKGDKHGFD
                     IVIGNPPYVRQEMIAPPPWTLPTSAGKTRTAGKSRRKPTKPSSSNPLPPPGRNSSRWR
                     GFEAPLT"
BASE COUNT         1624 a         2139 c         1817 g         1501 t
ORIGIN      
        1 ggctcgccag aaccatgcca tgagagagcc ccttcgtttt gttggcatcc cactggattg
       61 cagtcggtag gccaacgccc ctatcactcg attttgtgtg gaacaatagc cacaattgtt
      121 cccggaactc ccggggttgg tcccggtatc atttctcgca aggcagccgg atggtatccc
      181 ttcggcccgt atctctcttt aaatgggcgc cgccatggcc caaggatttt tccccttgtc
      241 gtaggctatg gacagatcaa taggtttgta attctgttca actcgggagg gctccagccc
      301 ccccggtacc cgagcaggta tagtagtttt caagatggtt gccttagatg attcggccac
      361 ccatccagct agcaaccgct tgagcggttc cgatattggc agcgctggac ggggacctac
      421 attccagtgc gtgctggatg ggaaactcgt ctcgaccaac tttttaggct tgtctactgc
      481 cccgatttgc aaagctgtga gagaggaaat gcaattccag tcgccaccaa ccgacccaat
      541 ccttgggacg gcttgaagag aagctcttat cggctttaga cgaaaacgcg ggggtaaaaa
      601 ggcaaaactc gggccaaccg aaaagctggt ggactggcgt cagccgtagt ttgtgcgagc
      661 tcccctcaca cggagggtca acacgaaaaa gtccccaaac gccatactcg caatcatttc
      721 tgcccccacg ttggacagat tttgtccaac cacggattta taaagaattg acaatgacgt
      781 ttcggaagat gaaaaatact gctattctca atgcggaagg atgggtcgga aagcgggagt
      841 gggcctggtg ggcaagagcg ttgtatcacc atgtttagga acgcgctctg ttcttcgata
      901 gggcggaggg tcaccgtccg cgccccataa acttggggac cacgattcac gacgtcgccc
      961 agtaccccag cttgggaacg tcggctaacg ataagcgtag gttggcccaa caaggaatgt
     1021 gttctcggct ccgggctggc ccaccattgg tggcaccatc atcaccgcgt cgatccctat
     1081 tgcatcagga ggggacgacg ccacattgcc gagacgatat gggccgacgc acggagtaag
     1141 gagaaggtta acagcaagaa ccgagaaagc taaggtcatg aagctcgccc gagttctatt
     1201 agtgtaaagc gagcggtgtg ctaagtctcc agctatggaa agggaatcgc tacgacgctg
     1261 tgcggggaat ttggaaacct gctccgggct cttttggcgg tccgaccgtg gcgagatggt
     1321 acgactctac cgtaacattc gtagttaaaa accgtaggag gacaccgtgc cacaaatctt
     1381 cgacaacgtc acgctaaagc tcatcgaggg attggagaag gcctttgaca aggccgtggc
     1441 cgcctccttc tgcatcggct atctcaatct tcgcggttgg gatcaggtag caggtctggt
     1501 ggaaacgttg caaggcgggg acgaggacca cgcctgtcgg gtgcttgttg gaatgtatcg
     1561 ccccccggat gaggaaatga gggcgcttgc cagtttgagg cttgagcaag aggcaattga
     1621 tggtcaggta cgtgcgcgtc tgatatcccg tatcgaacag atctttaggg accagctcac
     1681 cgttggcgtg ccatctgcta gcgtggagca agcccttcgt ggcttggcgg cgcagctgcg
     1741 ggcccgaaag gtctttgtga aggcatttct gcagtatcct cttcacgcca aactatacct
     1801 ggtccaacgg gatgacccgg tcgcgccact cgtcggtttt gtcggcagca gcaacctaac
     1861 cttggccggg ttatcgcgcc agggcgaact aaatgtggat gtcgtcgagc aggacgccgc
     1921 caagaaattg caggcctggt ttgacgaacg ctggggcgat gagctggccg tggatctgag
     1981 cgacaaactc gccacactca tcgagactag ctgggctcgc agtaagttgg tacgccccta
     2041 tttggtctat cttaaaatcg catatcacct ctccgaggaa gccggagagg aagaatctcg
     2101 attccgtgca ccgcagatat ttgcggaaaa aggcacaccg ttactcaatt ttcaggaaaa
     2161 agccgtctcc ctggctatcc atcatctcca tcgccgaggt ggagtgctgt tgggcgacgt
     2221 ggtcggtcta ggcaaaaccc tcatggccac ggctatcgcc cgggtcctcc aagaggatga
     2281 cgatagcaac accttagtca tttgtcctcc caagctcttg cctatgtggg agcagtatct
     2341 tcaggactat gagatcactg gaaaggtcct cagtttgggc aagtgcgacg aactttctca
     2401 tctgcggcgc tatcgcttag tgatcatcga tgaaagccac aacttgcgca accgtaaggg
     2461 taagcgctac caggcgatac aagaatacat cgagcggaat gatgcccgtg tccttttgct
     2521 cactgcgacc ccttacaaca agcaatttac tgacttaagt aatcaattaa gactcttttt
     2581 ggacgaaaac gaggacctcc acatccgccc cgaacggttt ttccaacaat ggatcaagga
     2641 cggcaaaact gaggccgatt tcatcgctca atttggcact tccccatctt ccttgcttgc
     2701 cttcgagaaa agcccattcc ccgaggactg gcgagatctc atgcggctct acctggttcg
     2761 ccgcacccgc cactttatca tgaaaaatta cgccgagttc gatgagacac gtcgacgtta
     2821 ctttgtgcgt ctcaacggcg agcccaagta ttttcctgtt cgagagccca ggcgggtctc
     2881 gtttaagatc gatgagaacg acccttcgga tcagtacgcc cggttatttc gggacgacgt
     2941 cgtgcagatc atcgagcaac tggtgctgcc ccgctatggg atggctcagt tcctcatcga
     3001 gaacgccgat cgcttggcca acgaggacca aaaacgcatc ctagacaatc tcaatcgagc
     3061 gggaaagcgg ttgatcggat tttgtcgcac taatttgttc aagcgactgg aatccagtgg
     3121 ccatagtttt ctgctctcag tcaaccggca tatcttgcgc aatctaatcg cattgcacgc
     3181 gctccggaac gggcttccca ttccgatcgg cacgcaagac cctgccatgc tggataccgc
     3241 catcaatgac gccgacgacg agctcatcat cggcgctgaa aacgatgacc cgactgctgt
     3301 cgatgcccgc gagatcgacg ccgggcccgt tttgcagacg gccaccctgg ctgcatacca
     3361 ggctctggcc gcaaagacgt acaaggccta cgccaccaaa tttcgtaccc gtttcgactg
     3421 gctggatccc aagttctttc agccagagtt agccaacgtc ctggaaaacg acgcccaagc
     3481 cctgtttacc gtcctggagc aagccggacc gtggaacccc gatgccgatg ccaaattgac
     3541 cgcccttgtg gaactcctta cccaccacca ccccaaggaa aaggtcttga tcttcaccca
     3601 gtttgccgac acggcgaggt atttacataa gcaactcgca aagcgaggca taaccgacct
     3661 tggtgtggcg accagtgaga ttcacgatcc cgtttccctg gcacgccgtt tcagcccctc
     3721 caccaacggc ggactgcgaa atggagagac cgagttgcgt gtcctggttg gcacggatgt
     3781 gttggccgaa ggcctgaatc tgcaagacgc ccacattgtt gtcaattacg atttgccctg
     3841 ggcgatcatc cggctaattc agcgagctgg ccgtgtggac cgtatcggcc agaagcacga
     3901 caccatcctg gtctattctt ttcttccagc cgatggcgtc gatcgaatca tcaatttgcg
     3961 aacgcggctg tttcgccgct tgcaggagaa tcaagaggtc atcggcaccg atgaatcatt
     4021 tttcggcgaa gatgctgcca ccaagctccg cgatctttac acggaaaagg ccggtaccct
     4081 cgatgacgac caaacagatg acgacatcga tctcgtcagt cttgccctcc aagtctggaa
     4141 cagtgcctcg cccgaagaca agaaggccgc cctatcgctg ccggcgaacg tctttgccac
     4201 gcgagctctg cccgagactg ccgatcccag ccaacatcca cctggtgtga tcgcctacct
     4261 gcgttttccc catcgcgagg ataccttggt gcgtgtggat gagcacggca acctggtttc
     4321 ccagtctctc tccagcatct ttcgggccgc cgcctgcgcc cccgatactc cgcctctacc
     4381 ccggagtgaa agtcatcacg accttgtggc ccgttgtgtc gagatcgtaa cccaggctca
     4441 gatatctacc ttcggtctcc tgggtccatt gcgtggcgtc cgtaggaaac tctacgaacg
     4501 gctcgaccgc tatcggaagc ttcagcagga acaccccacc tcttccagcg atgaaatcct
     4561 cgaacgactc gacactttaa tagacctcat ctaccgctat ccgctcaagc gcgccgccca
     4621 ggaccccatt agccgtcaaa tccgtctcgg catcaccgac gacgccctgc tagaactcgt
     4681 ctggcgacat gctgaagata aaaccctttg tgaaatcacc gaagaagacg aagcctcgct
     4741 ctccgagcca cagattattt gctcgatagg ccttgtgaat gaccaatggg gagaaaacag
     4801 ccatgaatga cctgtttccg aaaatccagt cgcaactagc ccacaaactt tccgacaacc
     4861 tcgagacgct cttttgcagg acgctcaatt ggggtgcgcc gcgaggtatc actcgacgtt
     4921 cacttcaaat tcccgcaccg gtgaacaaaa ccctcacggc ccagccggta gcgcagcttt
     4981 ctggagttta cgtctatcgc atcgactggc cagaggaccg cctcccggga atcaccgccc
     5041 gtcgggccgt ccagcgcgca ctttcagtcc tgcatcctga gtacctagcc tgctacgtga
     5101 cgcagaacaa aaagcaagcg gcatttgttt gggcacaccg ccggctagac ggcaagacgg
     5161 agctccgcac acttccctac gaagtcggtt cgccagcccg gaccaccatc gaacgcctcg
     5221 ccgaaatcgc attcacactg gatgaactcc gccccaccgg tgagccgccc atcaccgccg
     5281 tcaccgacaa gcttagtagg gccttcagcg tcgaagccgt cacaagcgag ttctttgaag
     5341 cctacaaaac agtttttgcc gacttgcaac aaagacttta ccggctttcg gacgacaagg
     5401 tctggtcgca cgactacgcc ctccagctcc tcaaccgtct gatgtttctt tactttatcc
     5461 agcgcaaaca gtggctcggc ggcgaccccc gctttctcgc caatttctgg cgagcctaca
     5521 agacctccgg tcagcccaaa gacacctttt ttgaacagtg gctttccgtc ctgttcttcg
     5581 aggcattcaa caacagattc cacggcggcc atcgccaatt tcccgaccac atccgctctg
     5641 ctcttgccca agccccctat cttaacggtg gcttgttcac ccgcaatagt ctcgacaatg
     5701 cctaccacat caaagtgccc gacgagttct tcgccacgct gttcgaccgc ttcaatggca
     5761 gcacgcccgg cttcctcgag cgctacaact tcaccattac ggaatccaca cccctggata
     5821 tcgaggtggc cgttgatccc gagatgatcg ggaaggttta cgaaagcctc gtcaacatca
     5881 ccttcgaggg cctcaccgaa gaagatcagc gcggttccgc cggcatcttt tacacgccgc
     5941 gcgtggaaat tgacctgatg tgccgtctca gcctcgtgga cgctctggcc aacaaactcg
     6001 gcgagtcccg caaacccatg ctctacgacg cggtctttgc ctacgatgcc atcgacaaag
     6061 acaaggccga ccaggccctt gccgctcaga acctttggcc ggagcttaac cgcctgttga
     6121 gggaaattac cgtctgcgac cctgcctgtg gcagtggctc gttcctggtg ggcatgttgc
     6181 tggtgctcga cgatcttcag gcccgggcca acgcccaact tggtatcgag gaaacgcctt
     6241 acgaacgccg ccgtcgcatc atcggcgagc aactctatgg cgtggacgtt atggattggg
     6301 cggtccacgt tgccgaacta cgcctctggt tgcaattggt cgtcgaaacc gaactgaagc
     6361 ccgccgaact caaatttaga ccgctcctcc ccaatctttc tttcaaaatc cgctgcggcg
     6421 acagccttgt gcaagaaatt gccggcgtca atttcggcct acactactcc gatccggatg
     6481 tccccgcccc actcaaaggc cgcattaccc aacttaaggg caaaaaactc cgcttctacc
     6541 agggcgagac caacttgtcc gaaaccgagc tcaaggccga ggaactcgct atcttccgcg
     6601 acatcctcaa gcacaaacgc gacgccctac aacaggagat cgacaggctt acccgccaga
     6661 tcgaatctcg cgccgaacag ggtgaatttg ctgctttctc caccggcaga cccaaacagg
     6721 aacggctcaa cttccaaaaa caatggcggg cagaacgcga cgaaaagcaa gccgaactca
     6781 agcgcatcga aaaaaacctc gaggcccttt gcccaaccaa gcccgtgcct ttcgtctggg
     6841 atattgcctt cgtcgaaatt tttaaaggcg ataagcacgg ctttgacatc gtcatcggca
     6901 atccgcctta cgtccggcaa gaaatgatcg cccccccacc ctggacgctg ccgacttcgg
     6961 cggggaaaac tcggaccgct ggaaagagca gaagaaagcc tacaaaacca agctccagca
     7021 atccgttgcc gccgcctggc cgaaattcgt cgagatggcg gggctttgag gcgccgttaa
     7081 c
//
LOCUS       DAOPTR010000045         7004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_8911_length_7004_cov_3.217873, whole genome shotgun sequence.
ACCESSION   DAOPTR010000045 DAOPTR010000000
VERSION     DAOPTR010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 7004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7004
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8911_length_7004_cov_3.217873"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="PKI05_01590"
     CDS             complement(<1..1052)
                     /locus_tag="PKI05_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50916.1"
                     /translation="MATLLRFNVTCLVISAMALGLFVMPSRLGLANSPESQPDWPTLC
                     QQAVAEFREITPQDVQRARDELLFAVKVLDARLDRAGPEAEGWRDYLLWKAFSEELKK
                     DSPSLDVLGAVYQRLSAGHEGLGLTWFRRVRLALERFLRLSQAVTDKNAQPNYQTVLN
                     ELGKSLAAYESDASPETATRVQELLRWLETMGQAKSVREAVLNHFSHPNVTIRVSGHW
                     ISISSQARVDVREPVREWILGTDVRGTGHMVGWRKLQIIPCEQHALFAIRVEGVLDSD
                     TIGYNGPARIYSHSKTPFVSQKLIQFGPDGLTAQPARCDASVSTDIQDVDVTCRSQCV
                     EKIAWRRTFQQKPAAER"
     gene            1497..2831
                     /locus_tag="PKI05_01595"
     CDS             1497..2831
                     /locus_tag="PKI05_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50917.1"
                     /translation="MTPAVLAEGPQDPTAVSWDYSTAYRMAEEKRGLLVVAFVKPGQE
                     GSAKTFVQSLMGDSRLRGWLAAAVVVTGSPVEVRGEAIVLSDHAAFATLKGQPGLAVI
                     NFGVDPNNDEYGRVIWALQVKDEEWDGYVELMRVSLVELAVAAGIGQDVKRATVNLPE
                     RIPNNTQENDGVVAAAYLAPEQSSIWMTDYKKAVEKARNEKKMLLVYVRPQQPDQQVL
                     QFERKTLLDPLVMKELFKFVCVRVSEDYVIEENETKIKILDHPSLVEMANRPGLFIVD
                     YASTDAEYYGQVVSVFPFLNGRPYDVDQMGVILTLPPGTLTQRTMIYAVRVHPERPKS
                     ILGRPEPFLFKEAEKHSAYQARIRLLGHRFWETRFHRILQVLRNESTASEVCAQSWPG
                     QRLLEAALECVRCWRLSPGHWRAVSSYQDAYGYDIKKGSDGIWYATGVFANH"
     gene            complement(2997..4604)
                     /locus_tag="PKI05_01600"
     CDS             complement(2997..4604)
                     /locus_tag="PKI05_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50918.1"
                     /translation="MPDASTAKDPHFNPLCAWLGFPPDALPCDYYALLGLPRFESDTG
                     EINRAAETLIAHVREIRPGPYLAEWTQLLDTIRAAKACLCDPEKKQAYDQALRTRRIT
                     TLDVNEWSQSRVLTADVDNGPEPSTAHVAAQALQTSPSLSRHTTSVGPTANTETVAPS
                     ISLKHFPTRKKPNWLAQIFSVFFLLVSVILAYQMLTAPAWWGARLRVILAAAQLGPRN
                     QEQHPVPSVGNTGSQNSVPVQNPVPQQPSSSRPSAPAQLSDTKATPPLGETPGSANSE
                     NIPDREEDSGTPAFQPPASQSSAPTPPATGDYGPNHIAELPNNEVEFPNNEVQMPTFQ
                     EKQGEEPHEMSPSVESPTDYEAAPPDLRPEIRSGVVHVWQAMAKRNLEAARSKLDQLA
                     GQVTNEAEQQAVEVLDYLLKHVEEFWRALHKIVSTMQAGEEFAIGDAYIVVVEATSEE
                     LVIRAAGQNRRYLIRQMPSPLIRLIVSRRFRPGPEADALLGAFLAVDPEGSPQEAEQL
                     WRRANDGVLDVEALLEALKLRPEASSR"
     gene            complement(4985..5197)
                     /gene="yacG"
                     /locus_tag="PKI05_01605"
     CDS             complement(4985..5197)
                     /gene="yacG"
                     /locus_tag="PKI05_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HOA50919.1"
                     /translation="MKKIRCPVCGRMFDPETTEAMPFCSRRCRIIDLGRWLGEQYAIP
                     HVEKDEEDEDSETPRLSDHESPEDYY"
     gene            complement(5236..5970)
                     /locus_tag="PKI05_01610"
     CDS             complement(5236..5970)
                     /locus_tag="PKI05_01610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOA50920.1"
                     /translation="MSNNTRPVAVVTGGGSGIGEAIAKVLAQEGYIVAILGRTESKLR
                     RVVEDIQAIGEAAYYVCDVSDRTRVDQVFADIAQKMGAPRLLVNSAGINVVNRSMATL
                     APEDWDRVLAVHATGSYNCIRAVLPAMRDAGEGLIINISSIAGKRALELAGAAYCAGK
                     FAMTALGMAAGLEERHHGIRITNIYPGEVNTPILEQRPEPVPQERREQMLQPEDLAEI
                     VRTIVRLPSRAHVWEVVVTPLYQPYS"
     gene            6199..>7004
                     /locus_tag="PKI05_01615"
     CDS             6199..>7004
                     /locus_tag="PKI05_01615"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOA50921.1"
                     /translation="MARASRLSWSWLFIVGCWGICVFASGCGGLSDDAQAGDKTAQAV
                     GTVQVRAEDGVCKSLVDDRPVLEYRYPARGPKSYVSLLTTPSGVNVLRDSPPDHVHHH
                     ALMFAVGIDGVDYWAEGERCGIQVPEGDFQSVSERTNGHVSCRLSHTLRWQAPDGSAQ
                     LREKRTIELIQNPKAETATLVIWTSKLTVAEGRTQVELWGRPYFGLGLRFVESMDRNG
                     KLFNSLGGTGVEETNNHRAPWCAYTAQADGKRVTVAVLDHPQNPRAPATW"
BASE COUNT         1534 a         1832 c         2004 g         1634 t
ORIGIN      
        1 cgctccgccg ccggtttttg ctggaacgtc cgtcgccaag cgattttttc gacgcattgg
       61 ctccgacatg tcacgtccac gtcttggata tccgtggaaa ccgaggcatc gcaacgcgcc
      121 ggttgggcgg tcaacccgtc gggaccgaat tggatcaact tttgagagac aaacggcgtt
      181 ttggaatgcg aataaatgcg ggcgggaccg ttatagccga tggtatctga gtccagaacc
      241 ccttccaccc ggatggcaaa cagggcgtgc tgttcgcacg gaattatctg aagctttcgc
      301 catcccacca tgtgtcccgt accccgcaca tcggtgccca aaatccattc gcggacaggt
      361 tctcggacat caacacgcgc ttgggaactg atactgatcc aatggccact gactcgaatc
      421 gtgacgttag ggtgggagaa atggttcagc actgcctctc gcaccgactt ggcctggccc
      481 attgtttcta gccaccggag aagctcttga accctggtgg ccgtttcggg tgaagcatca
      541 gactcataag ccgctaagga ttttcccaac tcgttaagga ctgtttggta gtttggctga
      601 gcatttttgt ccgtaacggc ctgagataaa cggagaaaac gctcaagggc caatcgcacc
      661 cggcggaacc aggttagtcc tagaccttcg tgccctgccg agagtcgctg atagacggcc
      721 cctaggacat cgagagacgg agaatccttt ttgagttcct cggaaaaggc tttccaaagc
      781 aagtagtctc tccagccttc cgcttcaggt cctgcccgat cgagccgggc gtcgaggact
      841 ttaacggcga acaaaagttc gtccctggcc ctttgcacgt cctgaggcgt tatttcacgg
      901 aattctgcca ctgcttgttg gcacaatgtc ggccagtccg gttgcgactc aggactgttg
      961 gccaacccca accgagaagg catgacgaat aggcccaggg ccattgctga aataacaaga
     1021 caagtgacgt tgaagcgtaa gagtgtcgcc attgtacgag cctccaatgc gcgaaagggt
     1081 gtccggggcc atcccaccgc acgcggttcc accatagtcc gaccgttggg catcagctct
     1141 ttggaccgtt ggcacgaata tgtttctcaa atgttttact ttgccgagtc gctaaataca
     1201 atgccatttt ccgatagccc acaatcgtta tgcgccttgc aaatgataca gactgtgcat
     1261 ttaatgtaga ttcacacaca atggctgctc atggactttc tgggacggcc cggttttggt
     1321 cagtcatgtg aaggctttgc ttctggagag tacctgcgat gaactgccga actgcatcga
     1381 agtgttgtgc acctggcgtt tttcggcaat tggcacagag acatttggag gggttagggc
     1441 gaaaggtagg ccttcccact ctccttacga tatgcttgct cggctattgg agcgggatga
     1501 ctcccgcagt tctcgcggag gggcctcaag accccacggc ggtttcgtgg gactattcca
     1561 cggcatatcg gatggcggaa gagaaacggg gtcttcttgt cgtggctttt gtcaaacccg
     1621 gccaagaggg gagcgcaaag acgttcgtcc aatcgctcat gggcgactct cgtctgcgag
     1681 gttggctggc tgcagcggtc gtcgtaaccg gtagcccggt agaggttcgg ggtgaggcga
     1741 ttgtactgag tgatcatgcg gcatttgcta cgttgaaggg acagccgggc ttggccgtga
     1801 tcaatttcgg cgtcgatccg aacaatgacg aatatggtcg cgtgatttgg gcactccagg
     1861 taaaagatga agaatgggat ggctatgttg agttaatgcg ggtttccctg gtggagttag
     1921 ctgtcgccgc cggcattggt caggacgtta aaagggccac ggtaaacctg ccagaaagga
     1981 ttcccaacaa tacgcaggaa aacgacggtg tcgtggcagc agcatatttg gcaccggagc
     2041 agtcctcgat ctggatgacc gactacaaga aggctgtgga aaaggccaga aatgagaaaa
     2101 agatgctcct cgtttacgtt cgtccccagc agcctgatca acaagttctt cagtttgaac
     2161 ggaagacatt gttggacccc ctcgtcatga aggagctttt caagtttgtt tgtgtccggg
     2221 tttccgagga ttacgtcatc gaagagaacg agaccaagat caaaatcctg gatcacccca
     2281 gcctggtcga aatggccaac cgtcccggcc ttttcatcgt agattacgct tcgacagatg
     2341 ccgagtatta tggccaggtt gtgagtgtct ttccgttcct caatggtagg ccttatgacg
     2401 tggaccagat gggcgttatt ttgacgttgc cgccagggac actgacccag cggaccatga
     2461 tttatgccgt aagagtccat ccggaacggc cgaaaagcat cttagggcga cccgagccat
     2521 tccttttcaa agaggcagaa aaacactccg catatcaggc acggattcga ttgctgggtc
     2581 atcgcttttg ggagacacgt ttccacagaa tccttcaggt gctccgcaat gaatccacgg
     2641 ccagcgaagt ctgtgcccaa agttggccgg gacagcggct cctcgaggca gcgttggagt
     2701 gtgtccgctg ctggcgatta tcaccgggac attggcgagc cgtgtcgtct taccaggatg
     2761 cctacggata cgatatcaag aaaggttccg acggtatttg gtacgcgacg ggagtctttg
     2821 ccaatcactg atgttttttc cttatgccca caaaccacag cgagaagtgc ggagggcgtt
     2881 ctggcggctg tttcatgcta ggagccagag tttttaagtc actttcggca agacctgctt
     2941 gctcacgttg cgggccttga ggcgaaaggg gaaatagcag cgcgttattg aggcagtcac
     3001 ctagacgaag cttcaggccg cagtttcagg gcctcgagga gggcctccac atccagtacc
     3061 ccgtcgttag ctcgtcgcca caattgttca gcctcttgcg ggctaccctc gggatccacg
     3121 gccaaaaagg cacctaacag ggcatcggct tccgggccgg gacggaatct ccgtgagacg
     3181 atcaggcgaa tcagcgggct tggcatctgc cgaataagat agcgcctgtt ttgacctgcc
     3241 gcacgaatca cgagttcttc cgaagtggcc tccacaacca cgatataagc gtcccctatg
     3301 gcaaactcct caccggcctg catcgtggat acgatcttat gaagtgcccg ccaaaactct
     3361 tccacgtgtt tgaggagata atcgagcact tccaccgcct gttgttcggc ctcgttggtg
     3421 acttgtccgg caagctgatc caatttcgag cgggcggcct caagattgcg ttttgccatg
     3481 gcctgccaaa cgtggacaac gccagaccga atctctggtc gtaggtctgg tggagccgct
     3541 tcgtaatcag tgggcgactc taccgatggt gacatttcgt gcggttcttc cccctgtttt
     3601 tcttggaacg ttggcatttg gacttcatta ttcgggaatt caacttcgtt gttcgggagc
     3661 tcagcaatat ggttcgggcc ataatcgcca gtcgcaggcg gtgttggtgc cgatgactgc
     3721 gaggccggcg gttggaaagc cggagttcca gagtcctcct cacgatcagg tatgttttct
     3781 gagttcgcgg atccgggagt ctcgccaagg ggtggtgtcg ccttcgtgtc acttagttgg
     3841 gccggagccg aaggccgtga tgacgacggc tgttggggaa cggggttctg taccgggacg
     3901 gaattttgcg acccggtatt acccacggac gggaccgggt gttgctcctg gttccgtggc
     3961 ccgagttgcg ctgcggcgag aattacccgc aaccgagctc cccaccacgc aggggctgtc
     4021 aacatttggt aggcaagaat gaccgaaacc aaaaggaaaa agaccgagaa gatttgcgct
     4081 agccaatttg gtttttttcg cgtggggaag tgttttagtg atatgcttgg ggcgacagtc
     4141 tcggtatttg ccgttggacc aacggatgtg gtgtgtcgtg atagggatgg cgatgtttgt
     4201 aaggcctgag cggctacatg agcggtggaa ggctcgggtc cattgtccac gtctgccgtc
     4261 agtacacgag actgagacca ctcatttacg tcaagtgtgg taatgcgacg ggtcctgagg
     4321 gcctggtcat aagcttgttt cttttcggga tcacaaagac acgctttggc ggcccgaatt
     4381 gtgtcgagaa gttgggtcca ctcggcgaga tagggaccag ggcgaatctc gcgcacgtgg
     4441 gcaatcaggg tctctgccgc tcggttaatt tctccagtat cactttcaaa tcgcggaagc
     4501 cccaagagtg catagtaatc acacggcagg gcgtccggtg gaaagcccaa ccaggcacac
     4561 aaagggttaa aatggggatc tttggcagtc gaagcgtcgg gcatacacaa ccccatttgg
     4621 atgattggcg aatcactgat ggcagctgac cctttaaggg aatcggggaa ccaacaactg
     4681 cccagcagtt ccgaactgac gctggtctca cttatttcaa ttctagcgtg actttgcttg
     4741 gcatgaacgt ggcggatcgc tctgccaagg gaacccccgt ggtactgaga tgttggcgat
     4801 cacttctttc ctgtctttcc tgcgagtcaa gctggattgg ggcgaaaaag tatatcacaa
     4861 ctctctcttg aaagcgaagg ctcctcgtga aaactgggcg tccgcgggtt atacatccaa
     4921 aaacggccag aacgctttat actcttacct gtatcacggc tataacccaa agacaattgc
     4981 caagtcagta gtagtcctcg ggcgattcgt gatcggagag acgcggtgtt tctgaatcct
     5041 catcctcctc atctttttcg acgtggggta tcgcgtattg ttcgcctagc caccggccaa
     5101 gatcgatgat ccggcaccga cggctacaaa aaggcatggc ctcggtcgtt tcgggatcga
     5161 acatcctgcc acaaacaggg caacgaatct tcttcatagc ttgcggaaag gattctcaag
     5221 ccgacggtgt cttccttatg aatacggttg gtacagcggt gtgacaacga cctcccacac
     5281 gtgagctcgc gatggaagcc gcacaatcgt ccgcacgatc tcagccaggt cttccggttg
     5341 aagcatctgt tcccggcgtt cctgcggtac gggttcaggc cgctgttcca gtatgggcgt
     5401 gtttacttcg ccggggtaga tattggtgat gcgaattccg tggtgacgtt cctccaaacc
     5461 ggctgccatt ccgagggccg tcatggcaaa tttacccgca caataagcag caccggccag
     5521 ttccagggcc cgtttcccgg caatggagga aatattgata ataaggccct cgccggcatc
     5581 ccgcatagcc ggtaacaccg ctcgaatgca gttgtaactc cccgtggcat gcacggccaa
     5641 aacgcggtcc caatcttcgg gagcgagcgt ggccatgctc cgattgacaa cattgatgcc
     5701 cgcactattc accagtaaac gtggcgcacc catcttttga gcaatgtcag caaagacttg
     5761 atccacccga gtgcggtcac tgacatcaca cacatagtag gctgcttcac caatcgcttg
     5821 aatgtcttca acaacgcggc gtagcttaga ttccgtccga ccgaggatcg cgacaatgta
     5881 cccctcttgg gccaggacct tggcaatggc ttcgccaatt ccactacctc cacctgtgac
     5941 aacggcaaca ggtcgtgtgt tattgctcat gcgttccctt tcgtactgtg tgtgccggca
     6001 gtactatcgt attggcagag tgctcgaaag ctgtctcact gatgttacgg gtctttcagc
     6061 gctatggctg acatggccaa cttcttatta acctaatccc gcgttggaca actgaaaagg
     6121 gaacttgcaa cttgcgttgg tgaagattaa aactacccac ggttcgggtt ctttggctct
     6181 gacacttgga gaatgaacat ggcacgtgca tctcggctct cttggagttg gttattcatt
     6241 gtgggatgct ggggaatatg cgtctttgcc tcaggctgtg gcggtctgtc cgacgacgct
     6301 caggctgggg acaagacggc ccaggcggtg ggcacggtgc aggtccgcgc ggaagacggt
     6361 gtgtgcaaat ccctagttga tgatagacca gttctcgagt accgttatcc agcacgcggg
     6421 ccaaaaagct acgtcagtct cttgacaacg ccaagtgggg tcaatgtctt gcgcgattcg
     6481 cccccggacc atgtgcacca ccacgcattg atgtttgccg tcgggattga tggcgtggac
     6541 tattgggcgg aaggggagag atgcggtatt caggtcccgg aaggggactt tcagagcgtt
     6601 tcagaacgaa ccaatggcca tgtgtcctgt cgcttgtctc acacgttacg gtggcaggca
     6661 ccggatggta gcgctcagtt gcgcgagaag cgaaccattg aactgattca aaatccaaaa
     6721 gctgagacgg cgacgcttgt aatctggaca tcaaaattaa ccgtggccga aggccgaacc
     6781 caggtggagt tgtggggacg accctatttt gggttgggac ttcgctttgt ggagagtatg
     6841 gatcgcaacg gaaagctctt taatagtctg gggggtactg gggttgagga aaccaacaat
     6901 caccgggcgc cgtggtgcgc atacacagcc caggctgacg gcaagagggt gactgtggcc
     6961 gtgttggatc acccacaaaa cccgcgtgcg ccggcgactt ggtt
//
LOCUS       DAOPTR010000046         6978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_8956_length_6978_cov_3.518850, whole genome shotgun sequence.
ACCESSION   DAOPTR010000046 DAOPTR010000000
VERSION     DAOPTR010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6978
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8956_length_6978_cov_3.518850"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..663)
                     /locus_tag="PKI05_01620"
     CDS             complement(118..663)
                     /locus_tag="PKI05_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOA50922.1"
                     /translation="MNVLIDEATLQAGLDRLAAEIEKRYRGKPLTLVGVLLGSIVFLA
                     DLMRRLTIPYGVEMISSRQVTTEGNIQGPLFIQADLLAPEVAGRHVLIIDDIYDTGET
                     LWELIPQFDDFKVASVHAAVLLKKKNRQRVPLGPTLVGFEIPDVYVVGYGMDYRGYYR
                     NLPYIASLSPEEMKNLAQNGG"
     gene            complement(722..931)
                     /locus_tag="PKI05_01625"
     CDS             complement(722..931)
                     /locus_tag="PKI05_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50923.1"
                     /translation="MAKTQPNSDLDDKQRGLRTGPNVILVPNRGNVNDVHKSQRSERL
                     PASSALAMDDRYQVVFHTFVTMGKR"
     gene            968..2620
                     /locus_tag="PKI05_01630"
     CDS             968..2620
                     /locus_tag="PKI05_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50924.1"
                     /translation="MAEQLLFYSCYWRRRFPLFVWAASLIWAWASAFSPIKAQLTPEH
                     PAVRQAVERALAYLASREAEEGYLQIGARALRGLAFLKAGHAHDHPVIVKSAEEIIKQ
                     IPPSGLPKDFPMSNWDNYSTGLALIFLCNYDPQKFRQEIQLILQYSFFRQKNHGGWGY
                     PSNETGDTSQTQYQVLGLWEAIRAGFAVPSEVLDKVALWLVWTQDPSGGYGYQGNFPP
                     TPTLIPQSDVRIGMAVAGTGSAYVLASLLPGFRGTLERSDLPPALEKIAQARQAGRGR
                     LTIDPKLLRAAIQRGNAYIAKHFQVAPDRYRYYYIYTLERYFTFREAVEGPEPGLFDW
                     YTACAEYLLKTQQQDGSWMHQCGKVVDTSFAVLFLVRSTKQSMEGVRDYGPGVLVGSR
                     GLPKQTDNVIVKNGQVVALPLRTSAEDLAAVLNSPEMSDLVSAIEAVEAMPPEEIRLY
                     ISDQAEKLRQLAGSDQPSAKIAAIAVMGKSGDLSYVPTLIYALGDSNPDVVIAADRAL
                     RRLTRTFGSGLQPGFSETERQAAIEQWKKWYRQIGPSADWED"
     gene            2718..3572
                     /locus_tag="PKI05_01635"
     CDS             2718..3572
                     /locus_tag="PKI05_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50925.1"
                     /translation="MEVTYRQPWTDSSYGLRVTAFDRAVSMLLALLILLGLCVIGLFI
                     IWVTGRNSSAQTAVPIVLQPIGEGGLPLGSDLDINTENPGQLEDLEEPLLRDALASVA
                     DVVATRLADLDNPVLSDPDSVEAGGTKGELARGSGTGGNGVRRRWEIFFDKGNTLETY
                     ARQLDYFGIELGILLPGNQVQYVSGFSQPRPQVRVGPADAEKRFYLTWQRGELQQADR
                     ELLARAGIDAGNRLILKFLPPEVEARLAALERAYAGGRADQVSSTRFGIRREGNGFAF
                     YVISQQYR"
     gene            3664..4392
                     /locus_tag="PKI05_01640"
     CDS             3664..4392
                     /locus_tag="PKI05_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOA50926.1"
                     /translation="MKLDLTAIITYLGTADYIALAVVALWGAFCVVMVLRRIHRVRFA
                     NEQLQEEFLTRVEEGILRNDFESVARFCEEDPRALSQLTRLAIVHRRLGLSRLRTLLA
                     ERFQRDVLSDLEYRLSWIYTVIKSAPMLGLFGTVLGMMGAFAKLSGGEKVDPTKLADD
                     ISLALVTTAIGLAIAIPLVLCTASVSIQIKKLEDYVSSGLNRVLDVLRTAGIKQEITP
                     VPPVGPAVGSGAPRTPAERTFRKE"
     gene            4503..4991
                     /locus_tag="PKI05_01645"
     CDS             4503..4991
                     /locus_tag="PKI05_01645"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOA50927.1"
                     /translation="MPKSVWDKIAGEEAEAIVFRRPLQESVDFDITPMIDVTFLLLIF
                     FLVTSAIDVGKAVELPPARFGKGMNPSLALVITVAYQGEATPPAIYLADGKVGSPLSE
                     NAEQQRSDILAAVRESVSAGKPRVLLKAERKVKHKDIARILEIINEVPDVSTYIAVFE
                     RD"
     gene            5139..5663
                     /locus_tag="PKI05_01650"
     CDS             5139..5663
                     /locus_tag="PKI05_01650"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOA50928.1"
                     /translation="MGHSQSDDSLIDSSGATEPGETTLSFERVDVPEDAMDMTPMVDV
                     VFQLLIFFMMTAAFSLQKSLELPPSSPQDPTAQSMTLEDLDNESDFVIVRIDHENNIT
                     VDGQDAPSEHELLSKLREAKGGIARTGQAPRTGMLVLADPECRHETVVMVLDAGNAVG
                     MERVRLATVEDTRF"
     gene            5881..>6978
                     /locus_tag="PKI05_01655"
     CDS             5881..>6978
                     /locus_tag="PKI05_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50929.1"
                     /translation="MTDGRPEVPALAMTNIPAEELIKLLAEKDLVAPEVLEELRNQVA
                     MSRKGPKPIHAAALAKILVDKGYLSRLSAQRLMAKLESDWAQKTHRQKPDPLLLKPKP
                     ATPSPAEGEIRLADEEEPVIDLIALEDDASVPPPVPQKAATAQPPAEAKPQQTEPPVP
                     DEEKSQVDDLLSTLTPATTAPAAAPPVTWERRRKSPWESPLFLLGGGVLLLLVFVGIV
                     LLWSLNRRTGDELLSEADADFHSGSYTQAIYKYGKFLEDFPQHPQAGLARVRSGLARI
                     RQTLESSDPITAFQTVKQIVPEIAALPDFHAEADAEMAAVLPGLAQQIAQQALEKRDP
                     AIVSVGEEALSLCNRYVPKERFPAAKLADIQA"
BASE COUNT         1583 a         1893 c         1955 g         1547 t
ORIGIN      
        1 caatcaggct gggcgactac catttacagg ttgaccgttt ctggaaatag acaatgacgt
       61 atcaccgcgc caggtagtga cgcatcttgc cgtcttggta gctaagtggg tctcgcctca
      121 cccaccgttc tgtgcgagat tcttcatttc ctccggagag agcgaggcaa tgtaagggag
      181 gttgcgatag tagccccggt agtccatacc gtagccgacg acatacacat cgggaatctc
      241 aaaccccacc aaagttggcc ccaacgggac gcgctgccga ttcttctttt tcaataagac
      301 ggcagcatga accgaggcca ctttgaaatc atcaaactga ggtatcaact cccagagggt
      361 ttcacccgta tcgtaaatat cgtcgataat cagcacgtga cgcccggcaa cttctggggc
      421 tagaaggtcg gcctgaatga acagcggccc ttggatgttg ccttctgtcg tcacctgtcg
      481 cgaggaaatc atttccacgc catagggaat cgttagccgc cgcatgagat cggccagaaa
      541 aacgatgctt cctaaaagaa cgccaaccaa tgtcagcggc tttccccggt aacgtttttc
      601 gatctccgcg gccaagcggt ccagcccagc ctgaagtgtt gcttcgtcaa tcaagacgtt
      661 catagggtac ctctttacgt cctcctccac attcaccaca ttcgtaccgc cataatagaa
      721 attaacgttt acccatcgtt acgaatgtgt ggaatactac ctgataacga tcatccatgg
      781 caagagccga tgacgcgggc aaccgctccg aacgttgaga cttatggacg tcattgacat
      841 tcccacggtt cgggactagg atcacatttg ggccggttcg cagcccccgc tgcttgtcat
      901 cgagatcact attgggctgc gtttttgcca agggcgaggt cgttaagaaa gaaataggat
      961 ggtccgggtg gcggaacagc tcctcttcta ttcttgctat tggcggcggc gatttccact
     1021 gttcgtgtgg gcagcaagcc tgatatgggc ttgggcgagt gcattctctc ctatcaaagc
     1081 ccagctaacg ccagagcacc ctgctgttcg gcaggcggtc gaacgtgctc tggcctatct
     1141 tgccagccga gaagccgagg agggctacct ccagattggg gcccgggctt tgagggggtt
     1201 ggccttcctc aaagccggtc atgcccacga tcatcctgtt atcgtcaagt ccgccgaaga
     1261 gatcatcaaa caaatcccac ccagtgggct gcccaaggac tttcccatga gcaactggga
     1321 caactacagc accgggttgg cgctgatttt cctctgcaat tacgacccac aaaagtttag
     1381 gcaggagatt cagttaatcc ttcaatacag cttcttccgt caaaagaatc acggtgggtg
     1441 gggctatcct tccaatgaga ccggggacac ttcgcagact cagtatcagg ttctcggctt
     1501 gtgggaagcc atccgcgcgg gctttgccgt cccgagtgaa gtcttagaca aggtggccct
     1561 atggcttgtt tggacacagg acccaagcgg aggctacggc taccaaggga acttcccgcc
     1621 gacacctacc ctgatccccc aatctgacgt ccgcattggg atggcggtgg ccggaacggg
     1681 atccgcatat gtgttggctt cactcttacc tggtttccgg ggcacgctgg agcggagcga
     1741 ccttcctccc gctttggaaa aaatcgcaca agcccgtcag gcgggtcgtg ggcgattgac
     1801 cattgatccc aaactactgc gggctgccat acagcgcgga aacgcctata tcgccaagca
     1861 tttccaagtc gcacctgacc gctaccgcta ctactacatt tacactctag agcgatattt
     1921 cacatttcgc gaagccgtcg agggtccgga gcccggtttg ttcgactggt acaccgcctg
     1981 cgccgagtat ctcttaaaga cccagcaaca agatggaagc tggatgcacc agtgcggaaa
     2041 ggtggtggac acgtctttcg ccgtcctatt ccttgtccgc tcgactaaac agagtatgga
     2101 gggcgtgcgc gactacggtc ccggtgtttt ggtcggttct cgaggattac ccaagcagac
     2161 ggacaacgta attgttaaga atggtcaagt ggtggcatta cctctacgaa catctgccga
     2221 ggatttggca gcggtgctaa actctcccga aatgtccgat cttgtttcgg cgatcgaggc
     2281 tgtcgaggcg atgcctcctg aagagatacg cctctatatt tccgatcagg ctgaaaagtt
     2341 gcggcaatta gcgggcagcg atcagccctc tgccaagatc gcagcgatcg cggtcatggg
     2401 gaaaagtggc gatcttagct atgtgcccac gttgatttac gcccttggag actcgaatcc
     2461 tgacgtggtg atcgctgcgg atcgagcctt gcgtcgtctg acgcggacgt tcggttccgg
     2521 attgcagccg ggtttctctg aaacggagcg acaggcagcg attgaacaat ggaagaaatg
     2581 gtaccgtcag atcggccctt cggcagactg ggaggattga gaaggttgga actgttttcc
     2641 gcgatcggca atgcggcaag aaataaagga atctgagcga gagtgtggca cccatttttc
     2701 tagggtgaat gacccatatg gaagttactt accgtcagcc ctggaccgac tcgagttatg
     2761 gccttcgggt cacggccttc gatcgggcgg tcagtatgct tttggcgcta ttgatcctac
     2821 tcggactttg tgtcattggg ttattcatca tctgggtaac ggggcgaaac tcttcggcac
     2881 aaacggccgt tcctatcgtt ctgcagccca tcggcgaggg aggacttcct ttgggctcgg
     2941 atttggacat caacacggag aatcccggac aactggagga cctagaagaa cccctattgc
     3001 gagacgctct tgcctccgtt gccgacgtgg tggccacgcg tctggccgat ctcgacaacc
     3061 ccgtgctcag tgaccctgat tcggtggagg ctgggggcac aaagggagag ttagcccgtg
     3121 gttcgggcac aggtggaaat ggtgttcgcc gtcgctggga aatcttcttc gacaaaggga
     3181 acaccctcga gacttatgcc cgtcaattgg actactttgg cattgaacta ggtatcctct
     3241 tgccgggcaa ccaagtgcag tacgtttcgg gcttcagcca acctcggcct caggtgaggg
     3301 tgggaccggc cgacgccgaa aagcggttct atctgacgtg gcaacgggga gagttacagc
     3361 aggctgatcg tgaactgctg gcccgcgcag ggattgatgc tggcaatcgt ctgatcctta
     3421 agttcttgcc gcccgaggtc gaggcacgtc tcgccgcttt ggagcgggcc tacgccgggg
     3481 gtagggccga tcaggtgagc tccacgcgtt ttggaatccg ccgagagggc aacggtttcg
     3541 cgttctatgt gatctcgcaa caatatcggt aatgcgtctg tccgccttat gccggtgcga
     3601 tggaacgtta agggtgtcca ctcgaacctt tcgaaactat gactagcaga tgacggaaca
     3661 accatgaaac tggatttgac tgccatcatt acctacctgg gaaccgccga ttatatcgcc
     3721 cttgccgtcg ttgccctgtg gggtgcattt tgtgtcgtca tggtcttgcg aaggattcat
     3781 cgtgtccggt tcgccaacga gcaattgcag gaggaattct tgacgcgcgt ggaagaaggt
     3841 attctccgca acgactttga gtccgtagcg agattctgcg aggaagaccc tcgggcactc
     3901 tctcagttga cacgtttggc gatcgtccat cgccgtctcg gtttatcgcg tttacggaca
     3961 ttgcttgcgg agcgttttca acgcgacgta ctaagcgacc tggagtatcg cctgtcgtgg
     4021 atctacacgg tcatcaagag cgctcccatg ctcggcctct tcgggactgt gttgggaatg
     4081 atgggggcct tcgccaaact ctccggcggc gaaaaggtcg atcccacaaa attggccgat
     4141 gacatcagcc tggccctggt taccacggcc atcggtctgg ccatcgccat tcccctagtc
     4201 ctttgcacgg cttcggtcag tattcaaatc aaaaaactgg aggattatgt cagttctgga
     4261 cttaatcgcg ttttggatgt cttgcgaacc gcgggcatta agcaggaaat cactccggtt
     4321 ccgccggtcg gtccggcggt cggtagcggt gcccctcgca cgcccgccga gaggaccttc
     4381 cgcaaagagt aaacccagag aacggtatct gtgcgaccgc ttagcatgtt tgggaagaaa
     4441 gtccacacga cgattgctgg tcaacagcgt ctatcaaact tggctgtgac gacaggtgtg
     4501 ccatgcccaa gagtgtttgg gacaagatag ccggtgagga ggccgaggcg attgtctttc
     4561 gccgaccgtt gcaagagtcg gtcgatttcg acattacgcc aatgattgac gtgactttct
     4621 tactgctgat cttttttttg gttacgtcag caatcgatgt cggaaaggca gtcgagcttc
     4681 cacccgcccg atttgggaaa gggatgaatc cctcgctggc cctggtgatc acggtagcct
     4741 atcaaggaga ggcaactccg ccggcgattt atcttgccga cggcaaagtg ggtagcccac
     4801 tctcggagaa tgccgagcaa cagcggagcg acattcttgc ggccgttcgg gaaagcgttt
     4861 cggcgggaaa gccccgcgtc ctgctgaaag ccgagagaaa ggtgaaacat aaggatatcg
     4921 ccagaatcct agagatcatc aatgaggtac ccgacgtgag tacctatatt gccgtatttg
     4981 aacgggattg agcggtgggc aaaagaagac aatacgcgtg agcgcggaat ctcatgcatc
     5041 atgtcgtttg ccaacaatgc ggaacaaaaa tagcggtcgc ggacacccta cgcgaccggg
     5101 agatcatttg cccaaaatgc ggcgaaacca ttcgatttat gggccattct cagagtgatg
     5161 attcattgat cgatagttca ggagccactg agccagggga gaccacccta tctttcgaac
     5221 gagtcgatgt cccggaagat gccatggaca tgaccccgat ggtcgatgtg gtgtttcaat
     5281 tgctcatttt ctttatgatg acggcagctt ttagccttca aaagtctttg gaactaccgc
     5341 catcaagccc acaggacccc acggctcaaa gcatgacatt ggaagatctg gacaacgaaa
     5401 gcgattttgt catcgtgcgg attgaccacg aaaacaacat taccgttgac ggccaagatg
     5461 ccccgagtga acatgaattg ctcagcaaac tcagggaagc caagggggga atagcccgca
     5521 caggacaagc cccccgaacc ggcatgctcg tgttggccga tccggaatgc cgtcacgaga
     5581 cagtcgtgat ggtcctcgat gcaggcaacg cggtgggcat ggaacgggtc cgcttggcca
     5641 cggttgaaga cacaaggttt taactctaca agaccgcaag tgacgaggcc gacattaagt
     5701 cgtttcatca tggcacgacg agaatgaggt ggattgtcaa agccgaaaag taactaagag
     5761 tgttctggaa agccaagagg tgctcgacgc gtgaccgttt tcatgatcac tcgagacgct
     5821 aattaacatc aaccgtggca tgtcccggac agtgttttct gtcgaatctg attgagtgta
     5881 atgaccgatg gtcgtcctga agttccggca ctggccatga caaatatccc tgcggaagaa
     5941 ttgattaaac tgcttgcaga aaaagacctc gtggcccctg aggtcttaga ggagttgcgc
     6001 aaccaagtgg cgatgtcgcg gaaggggccc aaaccgatcc atgccgcggc ccttgccaaa
     6061 atccttgtgg acaaggggta cctctcgcga ctatcggcac agcggttgat ggccaaactg
     6121 gaaagcgatt gggctcagaa aacgcatcgc caaaaaccgg atcctttgct cttaaagccc
     6181 aagccagcga cacccagccc ggctgagggt gaaataagac tggctgacga ggaagagcct
     6241 gttatcgact tgatcgcctt ggaggacgac gcctctgtac ccccgcccgt tccgcagaag
     6301 gccgcaacgg cccagcctcc tgccgaggcg aaaccgcaac aaacggaacc gcccgttccc
     6361 gatgaggaaa agtcgcaagt tgatgacttg ctcagcacgt taactccggc gacaacagcc
     6421 cccgctgcag ccccccctgt gacctgggaa cggcgccgta aaagcccctg ggaatcgccg
     6481 ttgtttcttt tgggaggtgg cgttcttctc cttttggtct tcgtcgggat tgtgttactc
     6541 tggtcactta accgccggac gggtgacgag ttgctgtccg aagccgatgc ggattttcat
     6601 tccggttctt acacccaggc catctataag tacggcaaat tcttggagga tttcccccaa
     6661 catccccaag ccggacttgc acgggtccgg agtggcctag cccgaatccg tcaaacactg
     6721 gagagcagcg acccgatcac ggcctttcaa accgtgaagc aaatcgtgcc cgaaatcgcc
     6781 gcgctgccag acttccacgc tgaggccgat gccgaaatgg cggcggttct gcctgggctg
     6841 gcccaacaaa tcgcccagca agccttggaa aagcgggatc cagcgattgt ctcggtgggc
     6901 gaggaggcat tgagtctttg taaccgatat gtccccaaag aacgattccc cgcggcaaag
     6961 ttggccgaca ttcaagcg
//
LOCUS       DAOPTR010000047         6957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_8995_length_6957_cov_3.008114, whole genome shotgun sequence.
ACCESSION   DAOPTR010000047 DAOPTR010000000
VERSION     DAOPTR010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6957
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8995_length_6957_cov_3.008114"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="PKI05_01660"
     CDS             <1..847
                     /locus_tag="PKI05_01660"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOA50930.1"
                     /translation="LEPPRPQPVEPITEIRVTQFRDYLACPYRFYLRHILKVATVNDQ
                     IEELDPAGFGELAHQVLCEFARSRLRDSTDADAIAEHLIRRLEAIIKQRLSAAPLPAV
                     PIQIEQLKARLRAFATFQAEHAKQWRILHAEYEPKEPVLLDVDGKTIRLKGRIDRIDQ
                     HRETQWYLILDYKVTDTDNTKPEQSHHKKVGGQRVWIDLQLPLYRRLAGHVIKDRNVS
                     LGYILLPKKTESTGLREATWTEEDLAEAEETARDVVRRIWREEFWPPKEDAEGRFPEL
                     SPILP"
     gene            complement(1474..2001)
                     /locus_tag="PKI05_01665"
     CDS             complement(1474..2001)
                     /locus_tag="PKI05_01665"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOA50931.1"
                     /translation="MTTMTLPDQVIPLPADPRLAHALVAAVEDALTMCNARAKLVGIN
                     CVPIFEAGAITGVIGLHGKATGFLTFNTCEQVAIALTSGFLQEPVSGINGQVVDTVGE
                     LTNLIAGGLKKRVSDTPWGIQSVTVPSVIVGHNYQIVYARGITYLSATFEHENPEAFL
                     LDQRLLQAAVSLLRV"
     gene            complement(2246..3412)
                     /locus_tag="PKI05_01670"
     CDS             complement(2246..3412)
                     /locus_tag="PKI05_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOA50932.1"
                     /translation="MTTRTTRRRFLQQTAVTMAGLSVATPWLSAKAAESTAPTPPPVP
                     RWRGFNLLDFFQAMWRQVEGDFVKVTPTPEEDCQLIAELVFDFVRLPSDYWLWIDSDW
                     PRTKKLKPDDMFKIREATLEKIDQSIETCRKYGLHVNFNLHRAPGYCINDPEREPVSL
                     WSDKVAQEAFVYHWEMFAKRYRGIPAKELSFNLVNEAPSPRPGYMTAEDYCRIMTQAI
                     EAIRKVSPDRLIFVDGLNVGTKVVEGLIPLGVPQSVHAYYPAQISHYRASWVDRNSSF
                     PDPTWPIRNPDGSVKIGRKQLEEHFAPWGELRKKGIGVHCGETGCFNRTPHEVFLAWM
                     EDVLDILKTHDIGWALWNFRGSFGVLDSGRQDVAYEDFHGHKLDRKLLNLLQKY"
     gene            complement(3498..>6957)
                     /locus_tag="PKI05_01675"
     CDS             complement(3498..>6957)
                     /locus_tag="PKI05_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOA50933.1"
                     /translation="YIFDRGKTAQNEILSTGMEKDPVRLTAQATPQLKEGRQKDDRLA
                     VLDAQAFEGKPFDAAKGFTIEVVFRYFGQGSQLGNGRPNGMIFAQGDGYWHGLRLYCE
                     TPTGQLRFEMGRPQPQSAFGLSSPVGVPKGVWQHVAITWDGEQLRLYWNGVLTAAAAY
                     TGPYTPANGPLRIGFADAGIGSLQLQVAEWVAYSTVLPADEIAGHALAAVPGPLAAAG
                     SSARPAAWAETWKLATTSAAAGNWQDAEQQWATLAEQTDVPQAIRFLARFASAIAKQR
                     RGVTGPAIQTWAALSQDISTPEILRLNSAAMCVVRRRGIIWAVAAPELYEALLKSTDW
                     SAEERLSLLLLSGQAALQRGDPALAQQRFEEARSLPTTSKDIAWDLALRIAHCRRAAK
                     DWEAARQAYRQIAEATDAPMPVRSLALLAWASTYERASQWADAAKAYRDIGGSPILHP
                     VHRWEAEELAREMDRLAQGLPRRDPSWHRTPAPVVPNPGLVLHVAPQGNDANAGTTEA
                     PLGSLEGARNRIRQIKSTSGLPRGGITVLVHGGIYPVRQTLELTEEDSGTPDSPIVFS
                     AQENEVPIFTGGVVLTGFQPVRDANILRRLPEEARDKVVQVDVRANEVEDLGTLGSRG
                     FGLAGYPTHPWVDVYCNDQPGTLARWPNDGFVNIKGLIGGSGDQPGTWTVEGRPLTWD
                     LNNEIWMYGYWRYLWAGTTIRVASIDRKTGQLRTAGRSNYGFADGMPYYFFNVLEELD
                     QPGEWYLDRKSGILYLYPPQDESSLRVTLPVLAAPFVRTKNTSHVRFVKLTFECGRSE
                     GFVVQDGTDVALLGCRLLRLGTNGVVVLGGTRHLIMGCDIGTVGAGGIRLAGGDEKTP
                     TPGGHVVENCHIWDFTRVDRNYAPAVHVDGVGNVIRHNLFHDSPHHGIRLEGFDHLVE
                     LNEVHSVVYESDDQSGIDMFGNPISRGNVLRWNFWHHIGSGHNVAGQAGIRLDDMISG
                     VAVYGNVFYRAAGGQFGGLQIHGGKDNWADNNLFIACKAAVSFSAWSEDRWLKTVRDW
                     LENARRRGLDVSKPPLSEKYPELLQLEKDANRNFILRSLVISCGEFAIRDPGVNVFFD
                     NHAAEGDIGFVDPFHRQFGLKPDSPVFQWFDFRPIPFEEIGLYASPERATWPITHTVS
                     PKYVGK"
BASE COUNT         1526 a         2052 c         1955 g         1424 t
ORIGIN      
        1 cttggagccg ccgcgccctc aacctgtgga gccgatcacc gagatccgcg tgacacagtt
       61 ccgcgattac ctagcctgcc cgtatcggtt ttacttacgg catattctca aggttgcgac
      121 cgtaaacgac cagattgagg agctagaccc tgccgggttt ggcgaattgg cacatcaggt
      181 tctttgtgag tttgcccggt cgcgtctccg cgacagcact gatgctgatg ccattgcgga
      241 acatctcatt cgtcgccttg aagccatcat taagcagcgc ctttctgccg caccgttgcc
      301 ggcggttccc attcaaatcg agcaacttaa ggcacgcctg cgggcatttg ccacattcca
      361 ggcggagcac gccaaacagt ggaggattct ccatgcggaa tacgagccga aggaacccgt
      421 cctcctggac gtggacggca aaaccattcg gcttaaaggg cgaattgacc gcatcgacca
      481 acaccgcgaa acacagtggt atttgatctt ggattacaaa gtgaccgaca ccgacaatac
      541 caaaccggaa cagtcacatc ataagaaggt cggcggccag cgggtgtgga tcgaccttca
      601 gttgcccctc tatcggcgat tggccggcca cgtcatcaag gatcgcaatg tgtcactggg
      661 atacatcttg cttcccaaga agacggaaag tactggatta cgtgaggcca cttggacgga
      721 ggaagacctt gctgaggcgg aagagactgc ccgggatgtt gtgcgaagaa tttggcgaga
      781 agaattctgg ccaccgaagg aggatgccga aggccgattt cccgaactta gcccaatttt
      841 gccttaagcc gcacatgccg ttgacgagtc gagcgaggcc aaacggtggt ccggcaatac
      901 ctcaagaaaa aaattggcgg cctcttgggg tgtcgcaaaa taccacggct gtatagaaca
      961 tatcgccgct aaatggtggt ttattttgca acatgtgttc aattttgcta tagcaaaaaa
     1021 atacaggcga gcttggattt gttcgggcgg caaatccagc tcgcctgcgt aggggtggaa
     1081 gccggtactt tgggtccgtc gggtgggttc ctcgtctggg tggaggagag catcgcgtgt
     1141 tggggggtcc cgacggtccc tatccgtaaa aacggcctct tctaagtgcc ggccccaagt
     1201 ctcgggacgg gtctcgctgc ctcttctctg tccggttgca tcgtcggtgg ccaaacattg
     1261 ccaccgcctg gtcgtcgccc gtctctcgga cgtctttata tctagcaatc gccatgccaa
     1321 tcggcgaggt aataagcttt ggcagacata acaggggtaa ttccccgtgt tcgccctttt
     1381 gacaattcca cggatggtca ggcgcagggg cctgccccta cggtaagtct ggaggaacgg
     1441 cggccgtcac gttgcgggct ttggcctttc gcctcacacg cggaggagcg aaacggccgc
     1501 ctggagtaac cgttggtcca aaagaaatgc ctcgggattt tcgtgctcaa acgtcgccga
     1561 aagataggta atccctcggg catacacgat ctgataattg tgcccaacga tcacggaggg
     1621 tactgtcacg gactgaatcc cccagggcgt gtcagacacc cgcttcttca gcccgccggc
     1681 gatgaggttt gtcaattctc ccaccgtatc gacaacctgg ccattgatgc cgctcacagg
     1741 ctcctggaga aaccccgagg tcagggctat ggccacctgt tcgcacgtgt taaacgtgag
     1801 gaaacccgtt gccttaccat ggaggccaat cacgcccgtg atcgcaccgg cctcaaagat
     1861 gggcacacaa ttgatgccca ccagtttcgc gcgggcattg cacatggtga gggcatcttc
     1921 caccgcggca acgagggcat gggccaatcg cggatcggcc ggtagcggga ttacctgatc
     1981 gggcagtgtc atcgttgtca tcgggcgttc tctccatagc aggcagacgg ttattctctc
     2041 cgaagcatac ctctcgagtg gatcagacct ttagaaaacc atgggatatg aaacggcctt
     2101 gatcatccgt gtggggggta agacaaaaaa gaaaatgcgg ggctgatatc cccgcattcg
     2161 gcgcaatcgt taatgacgcc acaacttgca taggcaattg ttgaccgcag gcaacaccga
     2221 tgttgcccgc ggaagaccta tcacctcaat acttctggag aaggttgagg agtttgcggt
     2281 ccaacttgtg gccgtggaag tcttcgtacg cgacatcctg gcgtccggag tccagaactc
     2341 caaacgagcc gcggaagttc cacaaggccc agccaatatc gtgcgtctta aggatgtcga
     2401 ggacatcttc catccaggca aggaagacct cgtggggcgt ccgattgaag cacccggttt
     2461 ccccgcagtg aacgccaatc cccttctttc gcaactcacc ccaaggggcg aagtgctcct
     2521 ccaattgttt gcggccaatc ttcaccgagc catcagggtt gcgaatgggc cacgtaggat
     2581 cgggaaagct ggagttgcga tccacccacg atgcccgata gtgactgatc tgcgccggat
     2641 aataggcatg gacgctttgc ggcacaccaa gcgggatgag gccctccacc accttggtcc
     2701 ccacgtttaa accatcgacg aagatgaggc gatcgggaga aaccttacgg atggcctcga
     2761 tggcctgcgt catgatgcgg caatagtctt ccgctgtcat atagccgggc cgaggactgg
     2821 gggcctcgtt cactaggttg aaactgagtt ccttggcggg gatgccacgg tatcgcttgg
     2881 caaacatttc ccaatggtaa acaaacgcct cctgagcaac cttgtcgctc cagagggaga
     2941 cgggttctcg ttccgggtcg ttgatgcagt agcccggcgc acggtgaaga ttaaagttga
     3001 cgtgcaaccc gtactttcga cacgtttcga tggactggtc gatcttctcc aacgtggcct
     3061 cgcggatttt gaacatgtca tcgggtttca gttttttcgt tctcggccaa tcggagtcga
     3121 tccatagcca gtagtcggaa ggcaagcgaa cgaagtcaaa caccaattca gcgatgagtt
     3181 ggcagtcttc ctcaggagtc ggagtcacct tcacgaaatc gccttccacc tgacgccaca
     3241 tggcttggaa aaaatcgagc agattaaagc ctcgccagcg cggaaccggt ggcggggtag
     3301 gtgcggtcga ttcggcggct tttgctgaca accagggcgt cgccacgcta agcccggcca
     3361 tggtcactgc cgtctgttgc aaaaatcgac gtcgcgttgt tcgagttgtc atgagcctcg
     3421 ctccttattc caagatgcaa acacgtcgcc agcacaaagg atatgttgtg ccaacaccta
     3481 cgccaacgcc ttccagttta cttgcctacg tactttgggg aaaccgtgtg ggtaataggc
     3541 cacgttgccc gctccggact ggcatacagg ccgatctcct cgaaggggat ggggcgaaaa
     3601 tcgaaccact ggaaaacagg ggaatccggc ttcaagccaa actgccgatg gaaggggtcc
     3661 acaaacccga tgtctccctc ggctgcgtgg ttgtcgaaaa agacgttaac gccggggtcc
     3721 cgaatcgcaa attcaccgca actgatgacg agtgaacgaa gaatgaagtt tcggttggca
     3781 tccttttcca actggagaag ctccggatac ttctcagaca gcggcggctt ggaaacatcc
     3841 agtccacgcc gacgggcatt ctccaaccag tcacggacgg ttttcagcca gcggtcttca
     3901 ctccaggcgg aaaaacttac agccgccttg caggcgataa agaggttatt gtcggcccag
     3961 ttgtctttcc caccgtgaat ttgcaggcca ccaaattggc cacccgccgc gcggtagaac
     4021 acattcccat agaccgccac accgctgatc atgtcgtcga ggcggatacc ggcctggccc
     4081 gccacattgt gtccactgcc gatatgatgc cagaaattcc aacgcagaac gttgccccga
     4141 ctaatcggat tgccaaacat gtcgataccc gattgatcgt ccgattcgta caccacgctg
     4201 tggacctcat tcagctcaac caagtgatca aagccttcca ggcggattcc atggtggggt
     4261 gagtcgtgaa agagattgtg gcgaatgacg ttcccaacgc catcgacatg gacggccggg
     4321 gcgtagttgc gatcaacgcg ggtgaaatcc caaatgtggc agttttccac aacatgaccg
     4381 cccggcgtcg gggtcttctc gtcgcctccg gcaaggcgga tgccaccggc tccgacggtt
     4441 ccgatatcgc aacccatgat gaggtgcctt gtcccgccca gaaccacaac gccatttgtc
     4501 cccaggcgga gtagccggca tccaaggagt gccacgtccg taccgtcttg gacgacaaac
     4561 ccctcagatc gcccacactc gaatgtcagt ttcacgaacc gaacatgcga ggtgtttttt
     4621 gtacggacga agggcgctgc aaggacgggc aacgtcaccc gcagcgacga ttcatcttgc
     4681 ggaggataca gataaaggat gccactttta cggtccaagt accactcacc cggttgatcc
     4741 aattcctcca gaacattgaa gaagtaatag ggcatgccat ccgcaaagcc gtaattcgat
     4801 cgaccggcgg tccttaattg gccggtcttt cggtcgatag acgccacacg gattgtcgtt
     4861 cccgcccaaa ggtagcgcca gtaaccgtac atccagattt cattgttcag gtcccaagtc
     4921 agcggccgcc cctcgacggt ccacgtcccc ggttgatcac ctgatccgcc gattaagccc
     4981 ttgatgttaa cgaagccgtc attcggccag cgggccagcg tccccggctg gtcgttgcaa
     5041 tagacatcca cccagggatg tgtcggatac ccagccaggc caaaaccacg ggatcccagc
     5101 gtcccaaggt cctccacttc attggcccgc acatcgacct gcacgacttt atcccttgcc
     5161 tcctcgggaa gacgtctgag gatattggca tcgcgaacag gttgaaagcc ggttagaacc
     5221 acccctccgg taaagatggg cacctcgttt tcctgggcag aaaagacgat gggcgaatcg
     5281 ggcgtgccgg agtcctcttc tgtgagttcc agtgtctgtc ttacgggata aatcccgcca
     5341 tggaccaaaa cggtgatccc acctcgtggc aggccgcttg tgctcttgat ctggcggata
     5401 cggtttcgcg ctccctccaa ggagccaagc ggtgcctcgg tcgttccggc attggcatcg
     5461 ttcccctggg gcgcaacatg cagcaccaat ccaggattcg gcaccaccgg ggcaggcgtt
     5521 cgatgccagc tagggtcgcg ccggggaagc ccttgggcta ggcggtccat ctcgcgcgca
     5581 agctcctcgg cctcccagcg atgaacagga tggaggatcg gtgaaccgcc aatgtcgcga
     5641 taggccttcg ccgcatccgc ccattggcta gcccgttcgt aggtcgatgc ccaggccaga
     5701 agggccaaac tccgcaccgg catgggcgca tcggtagcct cggcaatttg gcgataggcc
     5761 tggcgggccg cttcccaatc cttggccgct cgccggcaat gagcgatccg cagggctagg
     5821 tcccaggcta tgtctttgga ggttgtgggc aaagaacggg cctcctcaaa gcgctgctgg
     5881 gccagggcgg gatcaccacg ttgcagtgcc gcctgaccgg agagtagcaa tagtgacagt
     5941 cgctcctcgg cggaccagtc ggtcgatttc aacaaggcct cgtaaagctc tggtgccgca
     6001 accgcccaga tgatcccacg gcgacgaacg acacacatgg cggctgaatt gaggcgcaga
     6061 atctccgggg tcgaaatatc ctgcgacaaa gcggcccagg tttgaatcgc cggtcctgtc
     6121 accccgcggc gctgcttggc aatcgcactg gcaaagcgcg ccaggaatcg aatcgcttgt
     6181 ggcacatccg tctgctcggc caaagtggcc cattgttgtt cggcatcctg ccagttaccg
     6241 gccgccgccg acgtggtggc cagtttccaa gtctccgccc aggctgccgg tcgtgctgat
     6301 gacccagccg cggctaaagg accgggcacc gccgccagcg cgtgaccggc gatctcgtca
     6361 gcgggaagaa ccgtcgaata agccacccat tccgccacct gcaattgcag cgaaccgatt
     6421 ccggcatccg caaagccaat ccgcagtgga ccattcgcag gcgtataagg tccggtgtaa
     6481 gcagccgcgg ctgtcaaaac gccgttccaa taaagccgca gttgttcgcc gtcccaggtg
     6541 atggccacat gctgccacac acccttgggg acacccacag gcgaggaaag accaaatgca
     6601 ctctggggtt ggggacgccc catttcaaag cgaagttgcc ccgtcggtgt ctcgcagtaa
     6661 agtcgcaggc cgtgccaata gccatcaccc tgggcaaaga tcattccatt cgggcggccg
     6721 ttgcccaact ggcttccctg cccaaagtag cgaaagacga cctcgatggt gaacccctta
     6781 gcggcgtcga atggcttgcc ctcaaaggcc tgggcatcaa ggacggctag tcgatcatcc
     6841 ttttgccgtc cttccttaag ttgcggcgtc gcttgggccg tcaagcgaac gggatccttt
     6901 tccatgccgg tactgaggat ctcgttttgg gcagtcttcc cacgatcgaa aatgtaa
//
LOCUS       DAOPTR010000048         6954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_9002_length_6954_cov_4.241629, whole genome shotgun sequence.
ACCESSION   DAOPTR010000048 DAOPTR010000000
VERSION     DAOPTR010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6954
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9002_length_6954_cov_4.241629"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..>1524)
                     /locus_tag="PKI05_01680"
     CDS             complement(129..>1524)
                     /locus_tag="PKI05_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOA50934.1"
                     /translation="THREKAECRQDEEAALGLRSTTLPPLRLSLNFCWTFFGNITQAL
                     SRLLLLVVIVKWEGLETVGLWVLTTSLCSSVFTCCELGLRPLLICDVRRVHPFQDYYS
                     LRLVLALAGAVAVVAFGGFSYGPGTALGLLAIVAVGRTFDSLSDICYGLLQREERMDR
                     IGAGMALRYAGGMVGMLAALMLGYGLVIAALVDSLVAALVYWFWSRRNAKTLLAGFRR
                     PANPAVAFIVEDLRWSPPRRVWLSLLWLSIPLGIVAIEINLATNLPRYLVDSILGRES
                     LAIFATLLQFAAAGMIFVQALGNALAPRLTRYYQSNDARRFWGLLARFLGMTTLLGML
                     PLVLLWTESGRNILAWIFCHQLLEDPAAARWLALSVCLLYVTGPLGRGVTSVLRFHSH
                     VVIRGVTLGLMLALIPPLATTYGLSGAGMGLTLALLLTLPLYGWVIVKAWKIGGHSIA
                     LPQRDQRTTHRAAA"
     assembly_gap    1525..1534
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1580..2704)
                     /locus_tag="PKI05_01685"
     CDS             complement(1580..2704)
                     /locus_tag="PKI05_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HOA50935.1"
                     /translation="MKSQRHSLDKSIRYPRVVFIVGTGRSGSTVLDLFLGQHPTIQSA
                     GELCNVVKSGWVGGEFCSCGERVPLCRFWTEVRKIWEELTGAKPEDYESLRSLVEGNR
                     RTLWKMLRNDRPEMLLLSGKEPGEFLAYAQWTAALYRAIAEVSGKTLLIDSSKNPARA
                     LALVLMDHRLDLIDLYLIHLVRDIRGFVWSQRKSFQRDERAGVAHDLSPRPVWKSALV
                     WLFVNSISERVIARHDLERTLFLRYEDFANDTLQTSLKLASFLNIAAQPWLEMMTSHK
                     PLQAGHVVAGNRVRMQRHINIKPDNDWQTHLTTFQKVLCWFLAGWKARQYGYTWEPVA
                     ARTTTTGGHTLAGPHYVHSPRTTLDTVATPSENQYGLHKP"
     gene            complement(2853..4766)
                     /locus_tag="PKI05_01690"
     CDS             complement(2853..4766)
                     /locus_tag="PKI05_01690"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOA50936.1"
                     /translation="MSWIRRPFREQWRGAIRDLMGLAWLSLAFLVSYFVSYYVRFDGD
                     SWLWINKWFLNTVAYVCAVKLLVFWGFRLHRSWGRFLTFHDLVLLVEATTVALAAVAL
                     VDRLLLPPRMIPRGILLIDWGTTLLVLGGCRAAIRAVWDGGWHNFFTNASPVLIAGAD
                     ESGEELLRAISRYQATRHRVVGFLDRDSRRLGNRIGGIPVVGTFDQACQVATRLGVKE
                     ILLAGGALTGKEVRRLMEEARRASIEVKVLPSYEQLLAGRIAVQPRPIAIEDLLRREP
                     VTLEWKNIHRWLEGRTLLVTGSAGSIGAEIARQLLHYSPRCLVLLDRSETGQFFLERE
                     LKEIQHRLLANQKSVPELAVVLADVLDLPRMQSIFHQYRPEVVFHAAAYKHVPLMESH
                     PGEAFKNIVLATKNVADLAARFGAESFVLISTDKAVNPTSVMGACKRLAEMYVQSMTN
                     TTSTRFVTVRFGNVLDSAGSVVQIFRQQIAEGGPVTITDPRMERFFMTIPEAAGLVIQ
                     AGVIGESGQILVLDMGDPVKIIDLAMDMIRLSGLEVGRDIEIRVTGLRPGEKLFEELR
                     AAGERTVPTRHKKIRVALGSRMDRATLVRAIEQLRDWADSDPPRVIAELKRIVPGYGV
                     SQMPVPEHIRRAA"
     gene            complement(5431..>6954)
                     /locus_tag="PKI05_01695"
     CDS             complement(5431..>6954)
                     /locus_tag="PKI05_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOA50937.1"
                     /translation="AFHGLVDYVWYVPGLAAVVAILIACLVRLYQMTQLAAQHRTASA
                     TMSLASPSSSLTLRWTSFTVAFILCAAWQIPAAQRSFFAGIAEFSWHRFLALYRTADW
                     SDLRGAIQSTVASNVVSPGDEELMDSENPGLSASDAPAASTATTMGFQGEGPTQLATE
                     REMIDLLLKAIHRDPRRAEAHLRLAKAHLRMFHLIQEQNENPMPLSQIRDAVFLSQFS
                     SQEELDRWLQTAIGNHVTLLHSAKQEALSAVRLCPLLGEGYFILGQLSFLNGGREEDV
                     MAWFRQSLAVRPNDGELLFRVGAELALAGQYQEALELWRRAFRCGRLYQIRLLERLMG
                     SVPPGEIDQEITFLIETFEPDLEMLRYMYRQYRKRYPVASLDPLRRAYAVALTSQLRD
                     ERNPPSQRAELWLELHFLHLDAGEIQAALQCAEEAVRCNPLDYRAHHYLSLRLEEMGR
                     FAEAEEHVRWCLQRRPGHRSLQNRLHNLYQKRMKAEDEARTWRMAEAVSPGGYPPNR"
BASE COUNT         1650 a         1990 c         1942 g         1362 t
ORIGIN      
        1 agaacgtcct gcgggtagag atcgtgggtg ccaacgagaa ggccatcaag agttacatgt
       61 ttggcctcga tgatgtggta ttgactcccg caccgtaatg cgatttggct ttgcggagcg
      121 ccggcctttc acgcggcagc gcggtgggtg gtccgttgat ccctttgcgg aagggcgatg
      181 gagtgcccgc ctattttcca tgcctttacg atcacccaac catagagagg cagtgtcaac
      241 agcagggcca acgttagccc cattcctgcg ccggacagtc catatgtggt ggccaacggt
      301 gggatcaatg cgagcatcag tcccagcgtg acgccgcgta tcaccacatg agagtgaaat
      361 cgcaggaccg atgtcacacc tcgccccagg ggcccggtga cgtacagtaa gcacacggag
      421 agcgccagcc agcgggccgc agcggggtct tccagaagct ggtggcaaaa gatccaggcc
      481 aagatgtttc ggcccgattc agtccacaga aggacgagcg gaagcatccc caacaatgtt
      541 gtcatgccca ggaaccgcgc caacaaaccc caaaaacgcc gtgcgtcgtt ggactggtag
      601 tatcgtgtca gccgcggtgc cagggcattt cccagggcct ggacaaagat catcccggca
      661 gcggcaaatt gcagcaacgt ggcaaaaatg gccagcgact cgcgtccgag aatcgaatcg
      721 acgagatacc gcggcaaatt tgtcgcgagg ttgatttcga tggccacaat tcccaaaggg
      781 atacttaacc aaaggagaga cagccacaca cgccggggag ggctccatcg caggtcttca
      841 acgatgaacg caaccgctgg gtttgccggc cgtcgaaagc ccgcaagaag tgtttttgca
      901 ttacgacgac tccagaacca atagacgagg gccgccacta acgaatcgac cagggcagcg
      961 atcaccaagc cgtagcccaa catcaaggcg gccagcatcc cgaccatacc gccggcataa
     1021 cggagagcca tgcctgcccc aatacggtcc attcgttctt ctcgctgaag aaggccgtaa
     1081 caaatgtcag aaagggaatc gaatgtccgt cccaccgcga caatcgcaag gagtccgagc
     1141 gcggttcctg ggccgtaaga gaagccacca aacgccacca cggctacggc tcccgccagt
     1201 gcgagcacaa gacggagcga atagtagtcc tgaaacggat ggacccggcg gacgtcacaa
     1261 atcagcagcg ggcgtaatcc aagttcgcag catgtgaaga cagaactaca cagcgatgtg
     1321 gtcaagaccc aaaggccgac ggtttcgagt ccttcccact tgacgatgac aaccagaagc
     1381 agcagccgcg agagggcctg cgtgatattt ccaaagaacg tccagcaaaa attgagcgaa
     1441 aggcgaagag gcggtagggt cgttgagcgc aggccaaggg ccgcttcctc atcttgccga
     1501 cactctgctt tttctctatg cgtgnnnnnn nnnntaaaaa acatattgcc atgatcacac
     1561 gtttactggc caccagaggt cagggcttgt gcaagccata ctggttttca ctgggagtgg
     1621 ccacagtatc gagtgtggta cgtggactgt gaacgtaatg tggtcccgct aaggtatggc
     1681 cgccggtcgt ggtcgtgcga gccgcaacag gttcccaggt gtacccgtat tggcgtgcct
     1741 tccatccggc caaaaaccaa cacaagacct tttggaaggt cgttaagtgc gtctgccagt
     1801 cgttatccgg tttaatgttg atgtgtcgct gcatccgcac gcgatttcca gccacgacat
     1861 gcccggcctg gagtggctta tggctggtca tcatttccag ccaaggctgt gccgcgatgt
     1921 tgaggaaaga agcgagcttc aatgatgtct ggagtgtgtc gttggcaaaa tcttcatacc
     1981 gcaagaacag ggtacgctcc aaatcgtgcc gtgcgatgac cctttccgag atgctgttga
     2041 caaacaacca aacgagggca cttttccata cgggtcgtgg ggaaaggtcg tgagcaacgc
     2101 cggcgcgttc atcgcgctga aagctcttac gctgtgacca gacaaatccc cgaatatccc
     2161 ggacgagatg aatcagatag aggtcaataa gatccaatcg atgatccatc agcaccaagg
     2221 ccaaggcacg tgccggattc ttggaggagt cgatcagcag ggtctttccg ctgacctcgg
     2281 caatggcacg ataaagggcg gcggtccact gcgcgtaggc taagaactcg cccggttcct
     2341 tgcccgaaag aagcaacatc tcgggccgat cgttacgtaa catcttccac aacgttcgtc
     2401 tattgccttc caccaaggac cttaggctct cgtagtcctc gggtttggct cccgtgagct
     2461 cctcccagat cttgcgaacc tccgtccaga accgacacag gggaacgcgt tcgccgcagg
     2521 agcagaactc gccgccgacc catccgcttt tgacaacgtt gcacagctcg ccggcactct
     2581 gaatggtggg atgctgaccc agaaagaggt ccagcacggt cgatccgctc cgtcccgtcc
     2641 ccacgataaa gacaacacgc ggatatcgga tggatttatc caaggaatgt ctctggcttt
     2701 tcatagggcg tgctcacatc attgatgcaa gattttggag aatctatgac gtttcttgcg
     2761 ggtgctgacc ggtggtcgga tttgtgtttc ctttgtttcg cgtaagcggc gtgtcgacgg
     2821 atgacgatgt cacctcccga aaaacaggca ccttaggcag cccggcgaat gtgttccggc
     2881 acgggcatct gggagacgcc gtaacccggg acgatccgtt tcagctcggc gatcacacga
     2941 ggaggatcgg aatcggccca gtctcgcaat tgttcaatcg cccgcacaag ggtcgcccta
     3001 tccattcgcg aaccgagtgc cacacgaatc ttcttgtgcc gcgtgggaac ggtccgttca
     3061 ccagccgccc gaagctcctc gaacagcttt tcacctggtc gcaggcccgt cacccggatt
     3121 tcgatatcgc gtcccacctc taatcccgag aggcggatca tatccatggc caaatcaata
     3181 atcttcaccg gatcgcccat atcgagcacc aagatttgtc cactttcgcc aatcaccccg
     3241 gcctgaatga cgagaccggc ggcctctgga atggtcatga agaagcgttc catacgcgga
     3301 tcagtgatcg tgacgggccc cccttcagca atctgctgac gaaaaatttg gaccacgctt
     3361 ccagccgaat ccaagacatt cccaaagcga acggtcacga agcgggtgga agtggtgttc
     3421 gtcatggatt ggacgtacat ctccgccagg cgcttgcacg ctcccatgac gctggtggga
     3481 ttgacggcct tatcggtgga aatgaggacg aaggactccg cgccaaaccg agccgccagg
     3541 tcggcgacat tcttggtcgc cagcacaatg ttcttaaagg cctcgccagg atgggactcc
     3601 ataagcggga cgtgcttgta tgccgccgca tgaaatacca cctctggccg gtactgatgg
     3661 aagatgcttt gcattcgtgg aagatcgagc acgtcggcca agacaaccgc tagctcggga
     3721 acgctcttct gattggccaa caaccggtgc tggatctctt tcaattcccg ttccaagaaa
     3781 aattgccccg tttcactccg atccaataga acgaggcatc ttggcgaata atgcaacaac
     3841 tgccgggcga tttcggcccc gatgctgccg gcacttcccg tcacaagaag tgttcgacct
     3901 tccagccaac ggtggatatt tttccattcc agtgttacgg gttcccggcg aagtaggtcc
     3961 tcaatcgcaa taggccgcgg ctggacggca atccgacccg ctaatagctg ctcgtagctt
     4021 ggcaagacct tgacctcgat ggaggcacgg cgtgcttctt ccatcaatcg gcggacttcc
     4081 ttgccggtca aagcaccacc ggcgagtaag atctctttga cgccaagccg tgtcgccacc
     4141 tggcaagctt gatcaaatgt cccaacaacg ggaatccctc caatccggtt acccaaccgg
     4201 cgactgtctc ggtccaggaa ccccaccacc cgatgccgcg tcgcctggta gcgactgatg
     4261 gcccgaagca gttcctcgcc ggattcatcg gcgcccgcaa taagcacggg cgaagcattc
     4321 gtaaagaaat tatgccaacc accgtcccaa actgcccgaa tcgctgcccg gcatccccct
     4381 aacactaaca acgttgtccc ccaatcaata aggaggatgc cccggggaat catccgaggg
     4441 ggcaagagaa gccgatcaac aagggccaca gccgcgaggg cgaccgtcgt ggcctcgaca
     4501 agcaacacga gatcatgaaa tgtgagaaat cggccccagc tacgatggag ccgaaacccc
     4561 caaaaaacca gcaacttaac cgcgcagaca tacgcgaccg tgttcaagaa ccacttgttg
     4621 atccacagcc acgagtcccc atcaaatcga acgtagtaac tgacgaaata actgaccaaa
     4681 aaggccaagg ataaccaggc tagccccatg aggtcgcgta tggccccacg ccattgttcg
     4741 cgaaaaggcc gccttatcca cgacatcgac gagagccgat cgagtgcccg tgcgaggtaa
     4801 ttccccatca cggatcggag gctaaccgca gcgtcttgtg aatttgaacc gagttgcttt
     4861 tgatccgcaa ttggtttccc attcccactc gttggcccac cggctacagg atcaaagtca
     4921 cagtgactgc tcgcctcatc gtggacaaga cgtaacgccg gcagtgaacc gacgacagta
     4981 ggtcggtccg gctggctcga tggggaaaat tggggggatt tcgggtgtct cctaagctcc
     5041 acgccaacct ccatgtcagg tggtcattct taggcgtctc ggccgcaggg aatgaatacc
     5101 cgatggtaac ggatgtgaca agaccgtttc ttccttgggc aaacacagga tttgaaatta
     5161 agagtgcatc ccaagaagtc tctctctccc tctgggagag ggctggggtg agggcaagca
     5221 atcgtcggag tcgattgaga aaaccggcag cgattcgagc caccctcacc cccgaatgct
     5281 ctcccgctga acggaagagg ggagattatg ggtaggcttt tacgcaaaca caggatttga
     5341 aattaagaga gatatcgcgt gcagacgtcc caaaaagggt aacattgcac gggatgttcc
     5401 gtcgaacagc gaggacacga acggctgtct tcatcggttg ggtggataac cgcccggaga
     5461 tacggcctct gccatccgcc aagttcgggc ttcatcctcc gccttcattc ttttttgata
     5521 aagattgtga agccggttct gaagcgaacg atgccctggc cgcctttgca ggcaccagcg
     5581 gacgtgttcc tcggcctcgg cgaaccgccc catttcttct agccgaagtg ataaataatg
     5641 gtgggcccga tagtcgagtg ggttgcaacg cactgcctct tcggcacact gtagggccgc
     5701 ctggatctcg ccggcgtcaa gatggaggaa atgcaattcc aaccatagct cggcccgctg
     5761 ggaagggggg ttcctctcgt cacgcagttg acttgtaagc gccacggcat acgcccgacg
     5821 aagcggatcg agggaagcca cgggataccg tttgcggtat tgccgataca tgtagcgaag
     5881 catttcaagg tctggctcga acgtctcgat gaggaatgtg atctcctgat cgatttcccc
     5941 cggcggaacg gaacccatga gtcgctcgag taaacgaatc tgatacagcc gcccacagcg
     6001 aaaagcccgg cgccacaact ctaaggcctc ctggtactgc ccggcgagag caagctcggc
     6061 gcccacccga aagagcaatt ccccatcatt aggtcgcacc gccagggact gacgaaacca
     6121 ggccattaca tcttcctctc gaccgccgtt taaaaaactg agttgcccca gaataaaata
     6181 cccctctccc aaaagggggc acaaacggac agccgaaagc gcttcttgct ttgctgagtg
     6241 caaaagtgtg acgtggtttc cgatcgctgt ctgcaaccag cgatccaatt cttcttgcga
     6301 ggaaaactgg gaaaggaaca cagcatcgcg gatttgggag agtggcatgg ggttttcgtt
     6361 ctgttcctgg attaagtgaa acatccggag atgggccttg gccaatcgta aatgggcctc
     6421 cgcccggcga ggatctcgat ggatggcttt gaggagaagg tcgatcatct cccgctctgt
     6481 ggccagttgt gttgggccct ccccttggaa tcccatcgtt gtggctgtgg aggcggctgg
     6541 ggcatcggag gcggacaaac cggggttttc ggaatccatt agttcttcgt cgccgggaga
     6601 cacaacattg cttgctaccg ttgattgaat ggctcctcgt agatcagacc aatcggccgt
     6661 gcggtataga gcaaggaagc gatgccatga aaattcagcg atgccggcga agaagcttcg
     6721 ctgtgcagcc gggatttgcc acgcagcaca aagaatgaag gcgactgtga agctggtcca
     6781 gcgaagggtg agactggacg agggagaggc gagggacatg gtcgcggaag cagtgcggtg
     6841 ttgcgccgcc agttgggtca tctggtaaag ccggaccaga caggctatga ggatcgccac
     6901 caccgcggct aagccgggga cgtaccaaac gtaatcgacg agtccgtgga atgc
//
LOCUS       DAOPTR010000049         6932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_9037_length_6932_cov_4.017304, whole genome shotgun sequence.
ACCESSION   DAOPTR010000049 DAOPTR010000000
VERSION     DAOPTR010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6932
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9037_length_6932_cov_4.017304"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PKI05_01700"
     CDS             <1..740
                     /locus_tag="PKI05_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOA50938.1"
                     /translation="QFLGDYPAAQEEFVALLRAAERGTLCVAVACRIPPWHCENLDPR
                     IGSILQGGLTARLQPPGEDARRAILQMAFRQKGVTVTAAPLQLFAEELAGSPRQLLGF
                     ALKLRQAAGRGPVDYPAARAFLRRYSPRPATSIDRVAKATARYFGIRLKDLRGPGRSR
                     AEVLARNVAMYLAQRLTGASLQQIGHYFGGRDHTTVGHGCRKIEALSRSDPSMQQAIF
                     MIEERLIPGEPQTICSAADETHPSRRS"
     gene            complement(1189..2028)
                     /locus_tag="PKI05_01705"
     CDS             complement(1189..2028)
                     /locus_tag="PKI05_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50939.1"
                     /translation="MWRSILRQWAQQTAAEAFQARVEEMAHETAQPAADSPPRPIDVG
                     IVFALASESGGLEDRLEEKIWIRGEAGQVVIGRAGQKNVAIIVTGPGAKSAHVGTEAL
                     ILGHHPRWIVAAGFAGSLNEHVRPMELIIPNRITDLTGRALQVGLTLDADQTKGIHVG
                     PLLSVPELVRHPLERRKLAEEHHAVAVDLESFAVAEVCRRYQVPMWVVKIVTDGVDDQ
                     LPPEVSYLMKPRKWTEKLGAVAGALWNRPGSIRDLWQLNELALRASDRLAEFLLSMIR
                     SLP"
     gene            2412..3647
                     /locus_tag="PKI05_01710"
     CDS             2412..3647
                     /locus_tag="PKI05_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOA50940.1"
                     /translation="MRLWYYAKGPNRFGPIEEAELRRRLGTGEIAAEDWLWTEGMSNW
                     VKAAEWLRTAGDPVVEKPIVAEAVAGPSAAVVVTASPPPVNDLAAGSLSQRMLPVREL
                     YERIEREVRKVFVGQDELVLGTLTALFSGGHVLIESVPGLGKTLFVRTLGRVLGCKFN
                     RIQFTADLMPSDITGSPIFDVKTQEFIFRPGPVFTNILLADEINRSPAKTHAALLEVM
                     QELSVTVERTRHKLDRPFMVLATQNPIESEGTYNLPEAQMDRFMFKLIMDYPREEEEA
                     RILILHSVREDIDQRLQELSVVTNPEEIMSVTRLAGEVRVDPKLVNYINALVRKTRNW
                     PQILVGASPRAGLALMQAARTLAAFRGRDYAVPDDVVDLALLALRHRVILAPEAEVEG
                     YRVDDVLHEVIRSVEVPRL"
     gene            3644..5029
                     /locus_tag="PKI05_01715"
     CDS             3644..5029
                     /locus_tag="PKI05_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOA50941.1"
                     /translation="MTPILMFLGFSLPFVALLATAAAWGVYPSLRAVLLGLIPVTATL
                     GLVVLESAWVAVLIVDVAYLVIIIRDLFTVPRKNWLRCERNHAKVASLKKRHPVSLTI
                     TSLAPWMVKAVIRDGADRILQPTPPLIHVALRPGLRTVVDYELRPQRRGAFSMSAVYI
                     RGNSRYGLWFRQMTYPCETVLHVYPDIKQLSEYAMLARVNRLSLIGVRRVRRAGHEDE
                     FERLRDYTIDDEYRWIDWRATARRRKLTVKDFQQSQSQRVVFMVDCGRMMTNEWAGLS
                     MLDHTWNAVLLLAYVALKHGDSVGLLAFSDEIHAFLNPGGGKHQMARLINMAFDRFPR
                     MVESRYDLAFRYLAAHVRKRTLVVLVTHLIDDVNGRQVERYLTNLAGRHLPLGVFLRD
                     HTMFAPLSDGQKERDDLWKAAAAARILTWRHQVITSIRSKGALVLDVFPEEMTAPLVN
                     QYLEIKARRLL"
     gene            complement(5007..5852)
                     /locus_tag="PKI05_01720"
     CDS             complement(5007..5852)
                     /locus_tag="PKI05_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50942.1"
                     /translation="MVDLAQTRVVIRAREIGDLCDLALAAIRRDGWSLLRWWLLMVIP
                     YLVVVDSILVIFFRRLWEPEPEFSLIGYAFLISVAGLTPLSLLSGLLTLHLGMSVFVK
                     RPTFREVWRAWISRLGYMLLVQLMPFASRGPAIAEIVLLERLVPTTREGRAAIRRRLT
                     ALASYGLSINQLGAFFALLTFFIIGLTMAGMYFSFLTFETPWRWGWALVGFQLGACIA
                     GGFNRVVRFFLYLNSRIKYEGWDVELAARAAAQRLLRTNREVSSSQPLTPPTSNQRPK
                     AAVSP"
     gene            complement(5845..6804)
                     /locus_tag="PKI05_01725"
     CDS             complement(5845..6804)
                     /locus_tag="PKI05_01725"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HOA50943.1"
                     /translation="MVQVPDFLKQRQADWLELEHICHIANRVTERPTAEMVCRLSELY
                     RAACADLALAEAQQLSPEVLDYLHRLVGQAHNVLYRHPQSLPRQWLRAIFIEVPAYAI
                     RDKMIWLAFASFWGLFLLTAFLSRQSETFAEAVVGQELLDAIEDMYSRPFSDEYPTIV
                     AVGFYIMHNIGIALRCFALGILFVLPGLFILVFNAVTLGTVFGHMSVAAQADTFFEFV
                     TGHGPFELTGICLAAGCGMRLGFSLLETGELTRLASLRLAARQVAPVLLVACFFFVLA
                     AIIEGLVSPSATPVETKRWIATLSTVVLVVYFSTGFFTRPSHG"
BASE COUNT         1506 a         2023 c         2040 g         1363 t
ORIGIN      
        1 tccaattcct cggcgattac ccggcggcgc aagaggagtt tgtcgccctc ctgcgggcgg
       61 ctgaacgtgg cacactatgc gtggccgtcg cctgtcgtat cccgccttgg cactgcgaga
      121 atctcgaccc ccgcattggg tccatcctcc aggggggatt aacagctcgc cttcagcccc
      181 ccggcgagga cgcccgcagg gcgattctcc agatggcgtt tcgccagaag ggcgtcaccg
      241 tgaccgcggc tccgctccaa ctctttgcgg aagagctcgc cggctcgccc cgccagctcc
      301 tcggatttgc cttaaaactg cgacaggccg cgggtcgtgg cccggtggac tatcctgccg
      361 cgcgggcatt cctgcgacga tactcgcccc gtccggcaac ctctatcgac cgggtagcca
      421 aggctactgc gcggtatttc gggattcgcc tgaaagatct ccgtgggccg gggcgctccc
      481 gtgcagaggt ccttgcccgc aacgtggcca tgtacctggc ccaacgcttg acgggtgcca
      541 gcctccagca gattggacat tactttgggg gaagagatca caccactgtt gggcatggct
      601 gccgtaaaat cgaggccctt agccggtccg accccagcat gcagcaggcc atcttcatga
      661 ttgaagaacg gctcatcccc ggcgaaccgc agaccatatg ctccgcagcg gacgagacac
      721 acccgtcccg ccgcagttga tccgcagcca ttaacacccc gtgtcaatag ccgaccaagt
      781 tgttgacacc actaacagcg gacgcgaaaa ctcctctaaa tcatcaacac aaacgacaaa
      841 gggctaactc actgtcaaca gcacaacaac aggttacccc cagccactac tttattgtaa
      901 atggccgtca tcgcgcgact tgcgtgccaa cgcaccgcgc aactaacacc cggcgccctg
      961 gtacctataa tagtcagctg gcttacatat cctttatgat ttggcgcgtt ggttacaccc
     1021 gcccgctgga aacctcagac cgccatctgc tttcaagaga tatcggctga ggaggatagg
     1081 ttttttaagt gatttgggag ctccgggaaa cctcggtaag aagacagcga agccattgcc
     1141 caatccttgg taatttggga gatctgaact gaaggcatcg atttcacatc atggcaggga
     1201 gcgaatcatt gaaaggagga actccgcgag tcggtcgctg gcgcggagtg ccaattcgtt
     1261 gagttgccaa aggtccctaa tgctgcccgg tcggttccac aaagccccag cgacagcccc
     1321 gagcttttca gtccacttgc ggggcttcat caagtagctg acctccggtg gaagttgatc
     1381 atcgacgccg tccgtgacga tcttcaccac ccacatgggg acctggtagc gtcggcagac
     1441 ctccgccacg gcaaaactct caagatcgac agccaccgcg tggtgctctt ccgcgagctt
     1501 gcggcgctcg agcgggtgac ggacaagctc tggcacggaa agcagcggac caacatggat
     1561 gcccttcgtt tggtccgcgt caagcgtcag accgacctgc aaggcccggc cggtcaggtc
     1621 ggtgattcgg ttgggaatga ttagctccat tgggcgaacg tgttcgttga gagagccggc
     1681 gaaaccagcg gccacaatcc agcggggatg atgaccgaga atcaaggcct ccgttccaac
     1741 gtgggccgac ttcgccccag gaccggtgac aatgatggcc acgttctttt gcccagcacg
     1801 gccaataacc acctggccgg cttcaccacg gatccagatc ttttcttcca ggcggtcctc
     1861 cagacctcca ctctcggatg ctaaagcaaa gacgataccg acgtcgatgg gccgtggggg
     1921 gctatcagcg gccggctgcg cggtctcgtg ggccatttcc tccacgcgag cttgaaaggc
     1981 ctccgccgcg gtttgttgcg cccattgtcg aaggatcgat cgccacatcg gtatcgtcct
     2041 gtttcgagga ttccaaacga cgcgaatgtt gtccgcacgg gatgggaggg acggcgtcct
     2101 cgccgtcccg aattgcggtc accgaggacg atgaccctcc cgaggttgcc gaaggttggg
     2161 gcatagggga ggatgaccct tgggaattgt tatccttgtc tgcgtgacaa ccccttctat
     2221 tgtagagaaa atatggcgat gggagggatg gcgtgcctgc cgtccgagcc gccgtgcccg
     2281 cgcagggtca ccgaggacgg cgaccctccc gaggttgcca aggatgatct tggagttgcg
     2341 ggtgtagctc agaaagattc cccgttcgct gggaacggtt accgattttt caaatgtgag
     2401 gagtccctct gatgaggctt tggtactatg cgaaggggcc gaatcgcttt ggaccgattg
     2461 aggaggctga gctgcggcgt cgcttgggga ccggtgagat cgctgccgag gactggcttt
     2521 ggacggaggg gatgagcaat tgggtcaagg ccgccgaatg gctcaggacc gcaggtgatc
     2581 cagtcgttga gaagcctatc gtggccgagg ccgttgccgg accatcagcc gctgtcgtcg
     2641 taacagcgag tccgccgcct gtcaacgatc tagcagctgg tagcctttct caaaggatgc
     2701 tccccgtgcg ggagctgtac gagcgtattg agcgagaggt acgaaaggtc tttgtcggtc
     2761 aggatgagct tgttctgggg acgttgacgg cgctgttttc gggtggccat gtcttaattg
     2821 aaagtgtccc tgggctagga aagacgctct ttgtgcggac gttgggacgg gtcctcggct
     2881 gtaagttcaa tcgaatccaa ttcaccgcgg acctcatgcc gtcggacatc acgggttccc
     2941 ccatctttga tgtgaaaacc caagagttca tctttcgccc tgggcccgtg ttcacgaata
     3001 tcctcctggc ggatgaaatc aatcgctcgc cggctaagac gcacgctgcc ctcctcgagg
     3061 tcatgcagga actgagtgtc accgtagagc ggacacggca taagctcgat cgaccgttta
     3121 tggtcctggc gacgcaaaat cctattgaat cggaggggac gtacaatctt cccgaggccc
     3181 agatggaccg cttcatgttc aaactgatta tggattatcc gcgggaggaa gaagaggcca
     3241 ggatcctgat tctgcacagt gtgcgcgagg acatcgacca gcgcttgcag gaactgagcg
     3301 tcgtcacgaa tcccgaagaa atcatgagcg tcacgcgatt ggcaggagaa gttcgcgttg
     3361 acccgaagct ggtcaattac atcaatgctt tggtgcggaa aacgcgaaac tggccgcaaa
     3421 tcctcgttgg ggcttcgccg cgggcaggat tagctctgat gcaagcggca cggacgctgg
     3481 ctgccttccg tggtcgggac tatgcggtgc ctgacgatgt ggtcgatttg gccctactgg
     3541 ccctccgcca tcgcgtcatc ttggcaccgg aggccgaggt cgaaggctat cgggtggacg
     3601 acgtattaca cgaggtcatc cgctccgtag aggtcccacg gctgtgaccc caatcctgat
     3661 gtttctcgga ttctccctgc cattcgtcgc gctcctagcc accgcggcgg cgtggggggt
     3721 ttatccttct ctgcgagccg ttctgttggg cctcattcct gtgacggcca cattggggct
     3781 tgttgtgttg gaaagcgcct gggtcgccgt gcttattgtc gatgttgcct atcttgtgat
     3841 tatcatcagg gaccttttca cagttcccag aaaaaattgg ttgcggtgcg aacgcaacca
     3901 tgccaaggtg gcgtccttga aaaaacgcca tcctgtgtcc ctgaccatca catcgctggc
     3961 cccttggatg gtaaaggcgg tcatccgcga tggtgctgac cgcatcttgc agccgacgcc
     4021 gccgctgatt cacgtcgcgc tcagacccgg cttacggacg gttgtcgatt acgaacttcg
     4081 tccgcagcga cgcggggcct tttccatgtc ggccgtctat atccgcggca acagccgtta
     4141 cgggttatgg ttccggcaga tgacctatcc ctgcgagacg gtcctccatg tctatcctga
     4201 tatcaagcag ctttccgaat acgccatgct tgcccgggtg aatcggctca gtcttattgg
     4261 cgtgcggcgt gttcgccgtg ccgggcacga ggacgagttc gagcgcctgc gggattacac
     4321 catcgatgat gagtatcgct ggattgactg gcgcgccacc gcccggcgga gaaagctcac
     4381 cgtcaaggac ttccagcaga gccaaagtca gcgcgttgtc ttcatggtgg attgtggtcg
     4441 gatgatgacc aacgaatggg ccggtttgag tatgctcgac cacacctgga atgccgtgct
     4501 gctactggcg tatgtcgccc tgaagcatgg cgactccgtg gggctgttgg ccttctccga
     4561 tgaaatccac gcctttttga acccgggtgg gggaaagcac cagatggcgc ggctcatcaa
     4621 catggccttc gatcgctttc cgcggatggt ggaatcccgc tacgacctgg cgttccgata
     4681 tttggcggcc catgtccgaa agcggaccct cgtggtgctc gtcacgcact taatcgacga
     4741 tgtcaacggc cgacaggtgg aacgttacct caccaatttg gcgggacggc acttgccgct
     4801 gggggtcttc ctccgcgatc acacaatgtt cgccccacta agcgacgggc aaaaggaaag
     4861 ggacgaccta tggaaggcgg cggccgcggc gcgcatcctc acctggcgtc atcaggtcat
     4921 cacatcgatc cgcagtaaag gggcactcgt gctggatgtg tttccggaag aaatgacggc
     4981 cccgctcgtc aatcaatacc ttgagattaa ggcgagacgg ctgctttagg acgttgattg
     5041 gacgtaggcg gagtgagcgg ctgagaagag gagacctccc ggtttgtccg caggagtcgc
     5101 tgggcggctg cccgggcggc cagctcaaca tcccaaccct cgtacttgat ccgactgttg
     5161 agatacagga aaaaccgcac cacgcggtta aaccccccgg cgatgcacgc gccgagctga
     5221 aaaccgacaa gggcccaacc ccaacgccag ggagtctcaa acgtcaaaaa ggagaagtac
     5281 attcctgcca tggtaagtcc gataataaaa aacgttagca aggcaaaaaa ggcaccaagc
     5341 tggtttatgg ataaaccgta actggctaag gctgtaagtc ttcgccggat ggccgcgcga
     5401 ccttcccgcg tggtgggcac gagtcgctcg agtaaaacga tttcggcaat ggcagggccg
     5461 cgactcgcaa agggcatgag ttgcaccagg agcatgtaac caagccgaga gatccacgct
     5521 cgccaaactt ctcgaaacgt gggccgtttg acgaaaacgc tcatccccaa gtggagggtc
     5581 agtaaaccgc tgagcaaaga aagcggcgtc aggcccgcca cgctgatgag gaaagcgtat
     5641 ccgatgaggg aaaactcggg ttccggctcc caaagtcgac ggaaaaaaat gacgaggatc
     5701 gaatcgacca ccaccagata gggaataacc atcaacagcc accagcggag aagcgaccaa
     5761 ccatcgcggc ggatcgccgc cagggcgaga tcgcagaggt cgccgatctc ccgcgcgcga
     5821 ataaccaccc gtgtctgtgc gagatcaacc atggctcggt ctggtgaaaa aacctgtgga
     5881 gaaatagacg acgagcacga ccgtggaaag cgtggcaatc caacgtttgg tctctaccgg
     5941 cgtggccgac ggcgacacga ggccttcgat gatggcggcc aaaacgaaga agaaacaggc
     6001 caccaacagc accggggcca cttgtcgggc ggcgagccgt agggaagcca gccgcgtcaa
     6061 ttccccggtc tctagcagcg aaaagcccag ccgcatgccg catcccgccg ccaagcaaat
     6121 ccccgttaat tcgaaaggcc cgtgtcccgt gacgaattca aagaaggtgt cggcctgggc
     6181 ggccacgctc atgtgcccaa aaaccgttcc taacgtcaca gcgttgaaca ccaggataaa
     6241 caaccccggc aacacaaaca ggatacccaa agcaaagcac cggagagcaa tcccgatatt
     6301 gtgcatgata taaaacccaa ctgccacaat cgtcgggtat tcgtcgctga acggccgaga
     6361 gtacatatcc tcaatggcgt caagcaactc ttggcccaca acagcctctg cgaaggtctc
     6421 actttgtcgg gaaagaaaag cggtcaacaa gaaaaggccc caaaacgagg caaaagccag
     6481 ccaaatcatc ttgtcgcgaa tggcgtaggc tggaacctcg atgaagatcg cgcggagcca
     6541 ctgccggggt aggctctgcg ggtgacggta aagcacatta tgggcctgcc ccacaaggcg
     6601 atgtagataa tccaacacct ccggcgatag ctgctgagcc tcggctagtg ccaggtccgc
     6661 gcaggccgcc cgataaagct ccgacaaccg gcagaccatt tcggcggttg gtcgctccgt
     6721 cacgcggttg gcaatgtgac aaatgtgttc cagttcgagc cagtccgcct gccgttgctt
     6781 gagaaaatcc gggacctgta ccatggcatc acagtgtcca acaaggaaac ttcatgaggg
     6841 actactttga cttagagcgt atcccacaac ctcccctctc tcgttgggcg gaagaggggc
     6901 cggggatgag ggtggcttga atcgctgccg gt
//
LOCUS       DAOPTR010000050         6928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_9046_length_6928_cov_3.858432, whole genome shotgun sequence.
ACCESSION   DAOPTR010000050 DAOPTR010000000
VERSION     DAOPTR010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6928
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9046_length_6928_cov_3.858432"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PKI05_01730"
     CDS             <1..760
                     /locus_tag="PKI05_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50944.1"
                     /translation="LIATQCVEAGVDLDFPVVYRAVAPLEAIAQASGRCNRHGRRHVG
                     NLIVFRPADDRDPYPPGYGHAAEATCYYLRQLARDRPEKLPSLLADPDGIREYFEVFY
                     SMQGLADRSGILYEERELERAIRAGNFEMVATYYRLISQQTIHILVPYEEERFKKLLE
                     EIEQGRGKPGFIRRWCRQAASFTVDIFPPKKDSPSWYHLNPIEFFHSRQPTQGGTDWY
                     YLLPGVTDVSQTLHGLAYHPQLGLVLPEGEICVV"
     gene            787..1506
                     /gene="cas5c"
                     /locus_tag="PKI05_01735"
     CDS             787..1506
                     /gene="cas5c"
                     /locus_tag="PKI05_01735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HOA50945.1"
                     /translation="MTHVQQHTLEVWGDFACFTRPEMKVERYSYPCPTPSAVRGIFDA
                     IYFKPQFYWQPDRVELLTYPSYLPLRRNEVKEKINVASVKKWMSGSEPPEPILADADR
                     SITGTDQKGRTQRQTLALRKPRYRITAHIVPRPGYEKEQAACDAQFVRRASQGKCFHQ
                     PALGCREFVAFFRYVSQLDQEPPAVDYNQDLGWMLYDVFDIRTANGQDGSPCISLFRA
                     QIVHGVLDIPPYESELVRKPK"
     gene            1531..3387
                     /gene="cas8c"
                     /locus_tag="PKI05_01740"
     CDS             1531..3387
                     /gene="cas8c"
                     /locus_tag="PKI05_01740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HOA50946.1"
                     /translation="MLHHLYNYAVREGIILPPGFQSRAIKWLLVFSPDGTFIEAVDLS
                     GGQKKSKGREFPRVPQAPANWLQGGGRSNFLVESLATAVNWPDDEENRHKIQQKHDFF
                     VALLLQVAQATPSHWALPEVLPLAQSLQNNEVLTTIQGHLVEKKAKPTDIATLAITTT
                     KAVPRIFVEETSWHNWWQDFLIKIKQPEMPTGSGPRRRKQEIEQMCDFLTGELVQPAR
                     THPKVKGLADIGGLATGDVLIGFDKDAFSSFGLQQSTNAAMSEESATIYVTALNHIVA
                     RRCYRVAKPKQGSGVKIGYWYSGAVPEELDPALDILGELAETTVPNQHESHSSLEVDQ
                     AEMRARRALRALRDGERPDLGNFHYYAMTLSASSGRVIVRDWMEGNFRELVENVNAWF
                     DDLMIVSRDGKKLVQTFKFADLLGATVRELSEVTAPLAAAMWRAALKRMPIPFEVVAR
                     TLRRIEIDIIEDETPRHSRFALLKAFTIRCERVPAMAAELNEHETHPAYLCGRIMALL
                     GAIQREALGDVGAGVIQRYYAAASATPALVLGRLIRTAQIAHLPKIQEEGLRYWFENQ
                     LAELWTKMRSVPPRVLTLEEQTLFAMGYYHQMAARFKKTSQGGNESELAQTK"
     gene            3497..4471
                     /gene="cas7c"
                     /locus_tag="PKI05_01745"
     CDS             3497..4471
                     /gene="cas7c"
                     /locus_tag="PKI05_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HOA50947.1"
                     /translation="MSQPITSRYEFLYLFDCENGNPNGDPDAGNAPRIDPEDLHGLVS
                     DVAIKRRIRNYVQIAKGNQMPYAIFVEQACNLNKHIARAHEETNGKLPDTKGAKKEEV
                     EKAREWMCKNFYDVRTFGAVMSTGPNAGQVRGPVQVTFARSLDPVLPLDISITRMAVA
                     DDVSGAKSWRDYEKWEQEQKEDTLRTMGRKALIPYGLYLGKGFISAHLAEQTGFSTDD
                     LRLLFEAILGMFEHDRSASKGHMTVRPPVFVLKHVGTDTDPQQRKRQAMLGCAPAHVL
                     FEEIIEVKRKDGVTVPRSFEHYQVVVHNDRVPKGTELLRLPEDMDKLT"
     gene            4598..5233
                     /gene="cas4"
                     /locus_tag="PKI05_01750"
     CDS             4598..5233
                     /gene="cas4"
                     /locus_tag="PKI05_01750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HOA50948.1"
                     /translation="MFTDDELVPISALAQYVYCPRRAALLFLERQWADNVFTVEGRHV
                     HQRAHRTNLRERLPDGYAIHNLLLKSERLGLVGTADVVEFHEKDPAGPRVTVVEYKRG
                     KHKRSKQTEYEVQLCAQVICLEEMLNIQISRGTLFFAKSKHRYDVECHRTLRTIVEDT
                     TQKVRQLFQGTCTPAPQYGRKCRGCSVIDICFPKVVSRKNVQYYIKHIVQE"
     gene            5272..6303
                     /gene="cas1c"
                     /locus_tag="PKI05_01755"
     CDS             5272..6303
                     /gene="cas1c"
                     /locus_tag="PKI05_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HOA50949.1"
                     /translation="MKHHLNTLFVTTQGTYLRKSGEAVAVCIEKQIRLRIPLINLGGI
                     VCFGRVSCSPALLAACARAGISVSFFTEFGRFLAVCQGFTSGNVLLRRTQYRWADDPE
                     KTAILARMFVMGKIANTRSVLRRAAREHEDPAAIRDISQIADQLTQILSMLEHAGGVE
                     LVRGLEGQASAAYFSVFNHLILTQKDHLKFTCRSRRPPRDPVNALLSFLYSLLMADVR
                     GACEASGLDAGVGFLHRDRPGRPGLALDLMEEFRTILADRVALTLINRQQIKPKDFVN
                     QPGGTVVLTDAGRRRVLEEYQARKNETITHPFLNEKMTLGLVPHIQARLLARVLRGDL
                     DTYPPFVLK"
     gene            6316..6609
                     /gene="cas2"
                     /locus_tag="PKI05_01760"
     CDS             6316..6609
                     /gene="cas2"
                     /locus_tag="PKI05_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOA50950.1"
                     /translation="MLVVVTYDVSTKDKAGQRRLTRVAKKCLDYGQRVQNSVFECVVT
                     PEQWVTLRKELLDLIDPEEDSLRFYFLGSRNRHRVEHVGAKPSLDLEEGFLIG"
     gene            complement(6687..>6928)
                     /locus_tag="PKI05_01765"
     CDS             complement(6687..>6928)
                     /locus_tag="PKI05_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50951.1"
                     /translation="ESDSIGVGYQSFNPRPRVEGDLHVHLTVVEIDSFNPRPRVEGDP
                     PPCNPHNGRHLRSHFREPAPSVSATCLISRLDFRK"
BASE COUNT         1724 a         1802 c         1817 g         1585 t
ORIGIN      
        1 actgattgcc acacagtgcg tcgaagctgg tgtcgatttg gatttccccg ttgtctatcg
       61 cgcggtagct ccccttgaag ccattgccca agcatcaggg cgatgcaatc gtcatggacg
      121 gcgccatgtg ggtaatctta tcgtttttcg acctgccgac gatagagacc cttatccacc
      181 aggatacggg cacgcggctg aggcgacctg ctactatctt aggcaacttg ccagggatcg
      241 cccggagaaa ctgccatccc tcctcgcaga tcccgatggc attcgggagt actttgaggt
      301 attttattcg atgcaggggc ttgctgaccg aagtgggatt ttgtacgaag aaagggagct
      361 agaaagggca atccgggcag gaaatttcga aatggtggca acgtactaca gacttatctc
      421 tcagcaaacc attcatatac tcgttcctta cgaggaggag cgtttcaaaa aactcctgga
      481 ggagattgaa cagggaaggg ggaagccagg ttttattcgc cgctggtgcc ggcaagccgc
      541 atcgttcacg gtggatatct ttccgcccaa aaaggactcc cccagttggt atcatctcaa
      601 tcctatagag ttttttcatt ccagacagcc gacgcaggga ggaacagact ggtactatct
      661 gcttcccggc gtcactgacg tctcccaaac gcttcacggc ttagcctacc atccccagtt
      721 aggtttggtg cttcctgagg gtgaaatctg tgttgtttag cggtcattga aaaaggagat
      781 tctcgtatga cacatgttca acagcacact ttagaggtgt ggggcgattt cgcctgtttt
      841 acgcgtcccg aaatgaaagt cgagcgctac tcctatcctt gtcccacgcc aagtgccgtt
      901 cgcgggattt ttgatgctat ctacttcaag ccacagttct actggcaacc tgaccgcgtt
      961 gaattgctga cgtatcccag ctaccttcca cttcgcagga atgaagtcaa agagaagatc
     1021 aatgttgctt ctgtgaaaaa atggatgtct ggctccgagc cacccgagcc tatcctcgcg
     1081 gacgctgaca ggtctattac gggaacagac caaaagggta gaactcaacg gcagactctg
     1141 gcactccgca aaccgcgata ccgtatcaca gcccacattg ttccgcggcc cggttacgaa
     1201 aaggaacagg cagcctgcga cgcgcaattt gtgcgtcggg catcccaggg taagtgtttt
     1261 caccagcccg ccctgggatg ccgcgagttt gttgcatttt tccgctacgt ttcgcaactt
     1321 gaccaagaac ctccggcagt ggactataac caagacctgg gttggatgct ctacgacgtt
     1381 tttgacattc gaacggccaa cgggcaggat ggatcaccct gcatttctct tttccgtgcc
     1441 cagatcgttc atggtgtact tgacattccg ccttatgaaa gtgaattggt ccgaaaacct
     1501 aagtaggttc cgctttagga ggcatcgagt atgttgcacc atctctacaa ctatgccgtg
     1561 cgggaaggga tcatactgcc cccggggttt cagagcagag ccatcaaatg gcttctcgtc
     1621 ttttctccag acgggacatt cattgaagct gtggacctga gcgggggcca gaaaaaaagc
     1681 aagggacgag aattcccccg ggttccccag gcacctgcta actggcttca aggaggcggg
     1741 cgttcaaact tcctggtgga aagcttggcc accgctgtca attggcccga tgatgaagaa
     1801 aaccgccaca aaatccaaca aaagcacgat tttttcgtcg cactgcttct ccaggttgcc
     1861 caggcaacac cctcccactg ggccttgccc gaggttctcc ctctggctca gtcgcttcag
     1921 aacaatgagg ttttgacaac gattcaagga cacctcgtcg aaaagaaggc aaaaccgacg
     1981 gacattgcca ccctagcgat caccacgaca aaggcagttc cgcgaatctt cgtagaagaa
     2041 acttcctggc acaattggtg gcaggatttc ctcatcaaaa tcaagcagcc agaaatgccg
     2101 actggaagcg gtccgcgacg gcgcaagcag gaaattgagc agatgtgtga ctttctcacg
     2161 ggcgaacttg ttcaaccggc acgcacccac cctaaggtca aagggctcgc cgacatcggg
     2221 gggctggcaa caggtgatgt cctgataggt ttcgacaagg atgccttcag ctccttcggg
     2281 cttcagcaaa gtactaacgc tgcaatgagt gaagaaagtg caacaatcta tgtcactgct
     2341 ttgaatcata tcgttgctcg ccgttgttat cgtgtggcta agcccaagca gggttctggg
     2401 gtcaaaattg ggtattggta tagcggtgca gtcccagaag aacttgatcc tgccctggac
     2461 atcctgggcg aactggcgga aacgacagtt ccaaaccaac acgaaagtca ttcttccctc
     2521 gaagttgatc aagcagagat gcgagcccgc agagcactcc gagcattacg cgacggcgag
     2581 aggcccgacc ttggcaattt tcactactac gccatgactc tctctgccag tagtggacga
     2641 gtgatcgttc gcgattggat ggagggaaac ttccgcgagc tagtggagaa cgtcaacgct
     2701 tggtttgacg atctcatgat tgtttctcga gacggaaaaa agcttgtgca aacattcaag
     2761 tttgccgatc ttctcggggc taccgtgcgg gagctttccg aggtaactgc ccccttggcc
     2821 gccgcgatgt ggcgagcagc gttgaaacgg atgcctattc cttttgaggt ggtggcacga
     2881 actttacgtc gaattgagat cgacatcatc gaggacgaga ctcccagaca tagtcggttt
     2941 gctttgttga aggcctttac catcagatgt gaaagggtac cagccatggc agcagaactg
     3001 aatgaacacg aaacgcaccc agcttaccta tgtgggcgaa tcatggccct acttggggcc
     3061 attcagcggg aagccttagg agacgtaggt gccggcgtga ttcagcggta ttacgcagca
     3121 gccagtgcaa ctccagcgct ggtcctggga cggctgattc gcaccgccca aatcgcgcac
     3181 ttaccgaaga ttcaggaaga agggcttcgc tattggtttg aaaaccaact cgcggagctt
     3241 tggacaaaga tgagaagtgt cccaccgcgt gtgctcactc tggaggaaca gacactcttt
     3301 gcgatgggtt actaccacca gatggcagcc cgattcaaga aaaccagtca aggaggtaac
     3361 gaaagcgaat tggcacagac gaagtgactt cttgcagcca cgttagccaa tggtgtgaat
     3421 atgatagcct gacaataagg tagatgcttt gtggagtgtt tttaaagaac cttgcagaaa
     3481 tgaaaggagc ttgactatgt cgcaaccgat tacgagccgt tatgaatttc tctatctgtt
     3541 tgattgcgaa aacggaaacc ctaacggtga tcccgatgcg ggtaatgccc cgcggattga
     3601 tccggaggac cttcacggtt tggtttctga cgttgcgatc aaacgtcgta tccgcaacta
     3661 cgtgcaaatc gccaaaggca accaaatgcc ctatgctatc ttcgtcgagc aagcttgcaa
     3721 tctcaacaag catattgctc gggctcacga ggaaaccaat ggcaaacttc ctgatacgaa
     3781 aggagcaaaa aaagaagaag ttgaaaaagc acgcgagtgg atgtgcaaaa atttttacga
     3841 cgtccggaca ttcggcgctg taatgtccac ggggccaaac gctggtcagg ttcggggacc
     3901 tgtccaggtg acgttcgccc ggtctcttga ccctgtgttg cccttggaca tttcgatcac
     3961 acgcatggct gtggcagacg acgtgagtgg tgccaagtcc tggcgcgatt acgaaaaatg
     4021 ggaacaagag caaaaagaag atacgttaag gaccatgggg cgtaaggctc ttattcccta
     4081 tggattgtat ctgggaaagg gatttatcag tgcccatctc gcagagcaga ccggatttag
     4141 tacggacgac ttgcgtttgc tatttgaggc gattctcggc atgttcgagc acgaccgttc
     4201 ggcaagcaag ggccatatga cggttcgccc tcctgtcttt gtcctcaaac atgtgggcac
     4261 agacactgac cctcagcagc ggaaacggca agccatgttg ggatgtgctc cagctcacgt
     4321 ccttttcgag gaaatcatcg aagtgaaacg caaggacggt gttaccgttc ctcgttcctt
     4381 cgagcactat caagttgtcg ttcataacga tcgagttccc aaaggaacag agcttcttcg
     4441 cctcccggag gatatggata agctgacctg aagtcatcgc aaaccggtgc ataacgtgac
     4501 cgtttactat cgcggctacc gagcggaaga ccttttccgc ccggtagccg attcgtggtt
     4561 ctttatattt gctcacacca gatttggagc cttaatcatg ttcaccgatg acgagttggt
     4621 tccaatttcc gctttggccc aatatgtgta ttgtccacgt cgggcagctc tcttgttcct
     4681 ggaacgccaa tgggctgaca atgtgttcac agtcgaaggg cgacacgtgc accagcgggc
     4741 ccaccggacc aatctgcgag agcgacttcc cgatggctat gcgatccata accttctcct
     4801 taagtcagaa cgattgggcc ttgtcggaac tgccgacgtc gtcgaatttc atgaaaaaga
     4861 tcccgcaggg ccgcgcgtaa ccgttgttga atacaaacgg ggaaaacaca aacgttccaa
     4921 acaaacggag tacgaagtcc aactctgcgc acaggtcata tgcctggaag aaatgcttaa
     4981 catacaaatc tcccgcggta cccttttctt cgctaagtcc aaacaccgtt acgacgtcga
     5041 atgccatagg acgctgcgga caattgtgga agacacaaca caaaaggttc gccagctttt
     5101 tcaagggacc tgcacacctg caccccaata cgggcgaaag tgccgtggtt gctccgtaat
     5161 agatatttgc ttccccaaag ttgttagccg aaaaaacgtc caatactata tcaagcacat
     5221 agtccaagag taggtacgtg ataagggact ttggacgtaa ggatcgcaaa cgtgaaacac
     5281 catctcaaca cactttttgt aaccacacag ggcacgtatc ttaggaaaag tggagaagcc
     5341 gtggccgtct gtatcgaaaa acagatacga ctccgcatcc cactcatcaa cttgggtgga
     5401 atcgtgtgtt tcggtcgcgt gagctgctcg ccggcccttc ttgccgcttg tgctcgtgcc
     5461 gggatttccg tctccttctt cacagaattc gggcgtttcc tcgcggtctg ccaaggcttt
     5521 acctccggta acgtgctact acgaaggaca cagtatcgct gggcagatga tcccgaaaaa
     5581 acggccatcc tagcacgaat gttcgtcatg ggcaaaatcg ccaacacccg ttccgttctt
     5641 cgccgggcag cacgcgaaca tgaagacccc gcagccattc gcgatatcag tcagattgct
     5701 gatcaactga ctcagatact ttcaatgctt gaacatgcag gtggcgttga attggttaga
     5761 ggcttggaag ggcaggcaag tgcggcctat ttttctgtct ttaatcattt gatcctgaca
     5821 caaaaggatc atttgaagtt cacctgccga tcacgtcgtc caccacggga cccagtcaac
     5881 gcattacttt ccttcctata ctccctgttg atggcagacg tccgtggggc ctgcgaagct
     5941 tctggtttgg acgctggtgt aggattcctc catcgcgata gacctggacg tcctggtctc
     6001 gccctcgacc tgatggagga gttccgaacg atccttgcag accgcgtggc attaacgctg
     6061 attaaccgtc agcaaatcaa acccaaagac ttcgtcaatc agcccggagg gacagtcgtc
     6121 cttacagacg ctggacgcag acgcgtcctt gaagaatacc aggcgcgcaa aaacgagaca
     6181 atcacgcatc cttttctcaa cgagaaaatg acactcggtc tggtgcccca tatccaggcg
     6241 agattgctcg ccagggtttt acgaggcgat cttgatacat atcccccatt tgtcctcaaa
     6301 tgaaaggaga ataccgtgtt ggtcgtcgta acgtatgatg tgtcaacgaa agacaaggcg
     6361 ggccagaggc gactcacccg cgtggcaaaa aagtgcctcg actatggaca acgtgttcaa
     6421 aactcggtgt tcgagtgcgt cgtgacccca gaacaatggg ttaccttacg gaaagaatta
     6481 cttgatctaa tcgaccctga ggaagacagt cttcgctttt actttctcgg cagccgaaac
     6541 cgtcaccggg tggaacacgt cggagccaag ccgagcctag atctcgagga gggatttctt
     6601 atcgggtaac gatatggggg ccgcgtaccc cgagtgcttg caaaagtgct ggaggttggc
     6661 ggacgtagta acatgcgatg taatcatcac ttgcggaaat ctaggcgcga aataagacat
     6721 gtggcagaca cagaaggagc aggttcgcgg aaatgactgc gtaagtgacg accattgtgc
     6781 gggttgcaag ggggtggatc gccctccacg cgagggcgcg gattgaaact gtcgatttcg
     6841 actacggtta agtgaacgtg tagatcgccc tccacgcgag ggcgcggatt gaaactttga
     6901 tagccaacgc cgatcgaatc gctttctt
//
LOCUS       DAOPTR010000051         6925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_9049_length_6925_cov_3.725619, whole genome shotgun sequence.
ACCESSION   DAOPTR010000051 DAOPTR010000000
VERSION     DAOPTR010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6925
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9049_length_6925_cov_3.725619"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="PKI05_01770"
     CDS             complement(<1..845)
                     /locus_tag="PKI05_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOA50952.1"
                     /translation="MVDAVEQRYQERLKRYVTAMRKGKPDRIPIRPFAAEFTAVYAGY
                     TIQEVTHDYQKAFDAVIRCATDFDWDAVVPNMVYVWTGLVQAINLRYYAVPGTDLPPD
                     VCFQYLEPPEENAWMRPDEYDALIEDPTGFLYNVWLPRVSRDVVPPGQPNTFRNNMSF
                     VLGGMAMMDYFMALGRQIERLRRETGTVSAISGILKAPFDILADKLRGYKGLCMDLYR
                     QPDKVLKACEALAPHLLHVAKSGADPAKNVPIGLWMHRGCIPFLSPQQFERFYWPTLK
                     WIIESL"
     gene            complement(1106..2008)
                     /locus_tag="PKI05_01775"
     CDS             complement(1106..2008)
                     /locus_tag="PKI05_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009093951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOA50953.1"
                     /translation="MAKPRVLILRAPGTNCDIETAYAFEKAGAVTDRIHIRRLLEQPA
                     TIRSYQILCVPGGFSYGDDVAAGKILAVQLRHHLLHVLEEFHQADKLILGICNGFQVL
                     LKTGLIVPPDESGKPWATLTWNESARYEDRWVHLEVASDRCVFLRGIERLQLPIAHAE
                     GRFVVRQASGLDRLAQAGQIALKYALPMELKRMARQENAASRQSVEAAQMATRCLGGA
                     SLSALESAVAKANDQRLPYPWNPNGSMGNIAGVCDRTGRVLGLMPHPERFIDPTHHPR
                     WTRGEWHHPPDGLRLFINAVNYFA"
     gene            complement(2078..4984)
                     /gene="purL"
                     /locus_tag="PKI05_01780"
     CDS             complement(2078..4984)
                     /gene="purL"
                     /locus_tag="PKI05_01780"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008659064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOA50954.1"
                     /translation="MLWEVDLFPAEGQPDVAARQIRQDAHDLGIHKDWTILTCHGYLI
                     EGQLTEAQVRQVTEELLADPVVERSLVAPVGDWSLTAPPQPGMEVIYVLPKPGVMDPV
                     AQSAQSAIADFGWKADQVRTFRKYWVAGLSQAEIKRLCQKILANDAIEQVVLGPLPFR
                     TLEQGRPYEFRLITVPIRDMDDEALVRLSREGQLFLSLVEMQTIREYFRQQGRDPSDV
                     ELETLAQTWSEHCSHKTLAGRIRYQDDQRQIEFNNMLKETIFAATHEIRKSLGQDDWC
                     VSVFEDNAGVVRFDERYHVCFKVETHNHPSALEPYGGANTGIGGVIRDPMGTGLGAKP
                     ICSTDVFCFAPPDVSHDELPPGVLHPQRVMKGVVAGVRDYGNRMGIPTVNGAVYFDKR
                     YLGNPLVYCGNVGLLPCEKARKMPQPGDLIVAVGGRTGRDGIHGATFSSAELTTESET
                     LSGGAVQIGNAITEKMLLDVLLEARDRGLYNAVTDCGAGGFSSAVGEMGRDIGAEVEL
                     SHVPLKYEGLSYTEIWISEAQERMVLAVPEKHWPELKALFESEGVEATVIGRFVPTGR
                     LVLRYAGHEVGNLSMEFLHHGRPRVVREAFYRPPQVRPLIIKDNAEATAGEDLLAILR
                     SPNVCSKHWIIRQYDHEVQGGSVIKPLVGRWDDGPSDAAVLRPVLDSYRGLVIACGMN
                     PRYGDLDPYWMAASAIDEAIRNCVAVGADPRRIAILDNFCWGNTDRPETLGSLVRAAL
                     GCRDMAVVLQTPFISGKDSLNNEFRVSETETISIPPSLLISAMGQIDDVRRCVTMDLK
                     QPHNVIYLVGMTADEMGGSHWCLVRHQEGGRVPHCDPHRAKRIFSALHAAIEAGCVRA
                     CHDLSEGGLAVAAAEMAFAGCWGMELDLDHVPCETSSLAPIIRLFSESNTRFLCEVPP
                     ERQSHFESYFAGLPVAKIGMVTEDDRFVVRFAGRTLIDLPLRTLKEAWLSPLAW"
     gene            complement(4968..5813)
                     /locus_tag="PKI05_01785"
     CDS             complement(4968..5813)
                     /locus_tag="PKI05_01785"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOA50955.1"
                     /translation="MRRWKTISIVGVGLIGASIGQAVLRRGLADEVIGIARRQTTLRA
                     ARRVGAVSHTTIDLDRGVAQADLIIVCTPVGTIPDLVRRVAEACPERALISDVGSAKG
                     QIVAALDEGLPRGCRFLGAHPLAGSEKTGPSYSDPNLFEGRVTIITPTKNTRAEDFDA
                     LEDFWTGLGSVVIRMSPEEHDKALAMTSHFPHLVASLLAGMLPEPWFRLTGSGFFDTT
                     RIASGDVEMWKQICMQNRENLVGACQAFADELGRLARALAAGDESTLEQILLRGKKNR
                     DALGS"
     gene            5999..6862
                     /locus_tag="PKI05_01790"
     CDS             5999..6862
                     /locus_tag="PKI05_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOA50956.1"
                     /translation="MRKSERLMILGYNTNGLVHHEPTAALRGLAKIGYRGVALTIDHY
                     LLSPFNLYWERQLHELRDVLDRLGMYCVIETGARFLLDPWHKHEPSLISPDASGRVRR
                     LDFYRHALRCAVTLHADCLSLWSGNRHDEVPPEQAWEWLIQGLSHLCDEASAMGVPVA
                     LEPEPGMFIATTADYDELCRRAPSLGCLLTLDIGHVLCQEEGPISSIVEQYAPRIVNI
                     HLEDMRRGTHEHLMFGEGEVDLAEVFASLQKIGYKKGIYVELSRHSHLGLQAAKQAFD
                     ILSRYQSGMTQ"
BASE COUNT         1411 a         2083 c         1996 g         1435 t
ORIGIN      
        1 agcgattcga tgatccattt caacgtcggc caataaaacc gctcaaattg ctgcggcgag
       61 aggaaaggaa tgcatccgcg gtgcatccac aggccgatag gcacattctt cgccggatcg
      121 gcccccgact tggccacgtg cagtaagtga ggggccaggg cctcgcaggc cttgagaacc
      181 ttatccggct gccggtacag gtccatgcac agacctttat atccacgcag tttatcggcc
      241 agaatgtcga aaggtgcctt gaggatcccc gaaatcgccg acaccgtccc cgtctcacgt
      301 cgcagccgct cgatctgccg tcccagggcc atgaagtaat ccatcatggc catgccccca
      361 aggacgaacg acatattgtt gcggaaggtg ttaggctgac cgggaggaac gacatcgcgg
      421 ctgacccgcg ggagccacac gttatagagg aatcccgtcg ggtcttcgat gagggcatcg
      481 tattcgtctg gacgcatcca ggcgttttct tccggtggtt ccagatattg aaaacagaca
      541 tctgggggca ggtcggtgcc ggggactgcg taatatcgga gattgatggc ctggaccaaa
      601 cctgtccaga cgtaaaccat attgggcacc acggcgtccc aatcgaagtc ggtggcacaa
      661 cggattacgg catcaaaggc cttttgatag tcgtgggtca cctcttgaat tgtgtaaccc
      721 gcataaaccg ccgtgaactc cgcagcgaat gggcgaatcg gaatgcggtc gggcttgccc
      781 tttcgcatgg ccgtcacata ccgtttgagc cgttcctgat accgttgctc cacggcatcg
      841 accatggtga cccctcctcg agcgcattag ttggaaatta gcatcgtcgg gcgccgcatc
      901 cccgaaaccc ctccgaggac acgcccggat gatggcctcg ccataatcaa tgtcgcccag
      961 tatgccataa ccttccccgg gctgtcaatt gcccatcgtg atgagccgct gacgccgttc
     1021 atctataggg gcaacggttg tggttgttct tttttgattt cacgaacggg caaacatgcg
     1081 gcctgccccc aatgagaatt tcttctcagg caaagtaatt gaccgcgttg atgaataggc
     1141 ggaggccgtc cggcgggtgg tgccattcgc cacgggtcca gcggggatgg tgggtgggat
     1201 caatgaaccg ctcgggatgg ggcatcagtc ctaggacgcg gcccgtcctg tcgcacacgc
     1261 ccgcgatatt tcccattgat ccgttgggat tccagggata aggcaatcgc tgatcattcg
     1321 ccttggccac ggcggactcc aaggcagaga gactcgctcc tcccaaacag cgtgtggcca
     1381 tctgcgccgc ttcaacagat tgtcgagacg ccgcattctc ctgccgggcc attcgcttaa
     1441 gttccatagg cagggcatac ttcagggcaa tttggcctgc ctgggccaga cgatcgagcc
     1501 cactcgcctg ccggaccacg aagcgtcctt cggcatgggc aatgggtagt tgcaagcgtt
     1561 cgatgccgcg caggaacacg cagcgatcgc tggccacctc aagatggacc cagcggtcct
     1621 cgtatcgggc ggactcgttc caggtcagcg tcgcccaggg cttaccgctt tcgtcagggg
     1681 gaacgatcag gccggttttt agcaagacct gaaagccatt gcaaatgcct agaattagct
     1741 tatcggcctg gtgaaactct tccaggacat gcaaaaggtg atgccgtaac tggacggcca
     1801 gaatcttgcc cgcggccacg tcgtcgccat aactaaaacc gcctggcaca cacagaatct
     1861 gatagcttcg aatggtggcc ggttgttcga gtagccgccg aatgtgaatg cggtccgtca
     1921 cggccccggc cttttcaaag gcgtaggccg tttctatgtc gcagttggta cctggggcac
     1981 gtaaaatgag gactcgtggt tttgccatgg ggcctttctt tcacatcgct gttgccagtc
     2041 tttccgccat tttttctgcc gtgggtcacc gctccgctta ccaagccagt ggactcagcc
     2101 atgcctcctt gagtgtcctc aagggaagat cgattagcgt ccgcccggcg aaccgcacca
     2161 caaagcggtc atcttctgtg accatcccaa tcttggccac gggaagaccc gcgaagtagc
     2221 tctcgaagtg gctctgccgc tcgggtggga cctcgcagag gaagcgggtg tttgactcgc
     2281 taaagaggcg aatgattggg gcgagggatg acgtttcgca cggcacgtga tccagatcaa
     2341 gttccatgcc ccagcagccg gcgaaggcca tctcggcagc ggcgacggcc aatccgcctt
     2401 cgctgaggtc gtgacacgcc cgaacgcaac ccgcttcaat ggccgcgtgg agggccgaaa
     2461 agatccgctt tgcccgatgg ggatcgcaat gggggacacg tccgccttcc tgatgccgta
     2521 ccagacacca atgggagcca cccatttcat cggccgtcat tcccacaaga tagatgacgt
     2581 tgtgcggctg cttgaggtcc atcgtcacgc atcgccggac atcgtcgatc tgtcccatgg
     2641 cgctgatgag gagcgacggt gggatactga tggtttccgt ctcgctgacg cggaactcat
     2701 tgttgaggct atccttgccg ctgatgaatg gcgtttgcaa gaccacggcc atatcccgac
     2761 agccgagggc cgctcgaacc agggacccca gcgtctcagg tcgatcggtg ttcccccaac
     2821 aaaagttgtc gaggatggcg atccgccggg gatcagcccc cacggcgacg cagttgcgaa
     2881 tagcctcgtc aatggcggag gctgccatcc agtagggatc cagatcgccg tagcggggat
     2941 tcatgccgca ggcaatgaca aggccgcggt aagaatccag caccggtcgc aaaaccgcgg
     3001 catcggaagg accgtcgtcc catcgtccca ccaagggctt gatgacgctt cccccttgga
     3061 cctcgtgatc atactggcgg atgatccagt gtttgctgca cacattgggc gagcgaagga
     3121 tagccaaaag gtcctcgccc gccgtggcct ccgcgttgtc tttgatgatc agcggccgaa
     3181 cctggggcgg acgataaaag gcctcgcgga ccactcgggg ccgaccgtga tggaggaact
     3241 ccattgacag gttgcccact tcatgacccg cgtacctaag gacgaggcga cctgttggca
     3301 cgaatcggcc gataacggtc gcttccacgc cttccgattc gaagagggcc ttcaattcgg
     3361 gccagtgttt ttccgggacg gccaatacca tacgttcttg ggcttcggaa atccagattt
     3421 cggtatagga gaggccttcg tacttgaggg gaacatggga gagttccacc tcggccccga
     3481 tgtcgcgacc catctccccg accgccgaag aaaaaccgcc ggcaccacaa tcggtcaccg
     3541 cgttgtatag gccccgatca cgggcttcca ggaggacgtc gagcagcatc ttttccgtga
     3601 tggcgttgcc gatctgcacc gccccgccgg agagggtctc actctcggtt gtcaattcgg
     3661 cggaactaaa ggtagccccg tgaatgccgt cccggcctgt ccggcctccc acagccacga
     3721 ttaggtcgcc gggttgtggc atctttcgtg ccttctcaca gggcagcaac cccacattgc
     3781 cgcaatacac aagaggattc cctaggtagc gcttgtcgaa atacacggcg ccgttgaccg
     3841 tgggaatgcc catccgatta ccgtagtcgc ggacccccgc cacgacgcct ttcatgaccc
     3901 gctggggatg aagaacgccg ggcggaagct catcgtggga gacgtcgggc ggtgcgaagc
     3961 aaaagacatc tgtgctgcaa attggtttcg cacccaatcc cgtccccatc ggatcgcgaa
     4021 tcaccccgcc gatcccggtg ttcgccccac cgtagggttc cagggccgac gggtgattgt
     4081 gcgtttcgac cttgaagcac acatggtacc gctcatcaaa gcgaacgacg cccgcgttgt
     4141 cctcaaagac actcacacac cagtcgtcct ggccaagact tttgcggatt tcatgcgtgg
     4201 cggcaaagat cgtctctttg agcatgttgt tgaattcgat ctgccgctga tcgtcttgat
     4261 agcgaatccg gccggccagg gtcttgtggc tgcaatgctc gctccaggtt tgggcaagcg
     4321 tctccaattc cacatcgctg gggtcacgac cttgttgacg aaagtactcg cggattgtct
     4381 gcatctccac caaggagaga aacaactgcc cctcgcgact gagccgcacc aaggcctcgt
     4441 catccatgtc gcgaatgggc accgtgataa gacggaactc gtagggccga ccttgttcca
     4501 aggtgcgaaa aggcaacggt cccaggacga cctgctcgat ggcgtcgttg gccaagattt
     4561 tttgacagag ccgtttgatt tctgcctgcg acaggccagc tacccagtat ttgcgaaagg
     4621 tccggacctg gtcggccttc catccaaaat cggcgatggc cgattgggca ctttgggcaa
     4681 cagggtccat gacccccggc tttgggagga cgtaaatgac ctccatgccc ggttgcggtg
     4741 gcgccgttaa cgaccaatcg cccaccgggg ctaccaaact acgttccacg acggggtcgg
     4801 cgagtaactc ctcggtcact tgccggactt gagcctccgt caactggccc tcgatgaggt
     4861 agccatgaca cgtgaggatc gtccagtctt tgtggattcc caggtcgtgg gcatcctgcc
     4921 gaatctggcg ggccgccaca tcgggttgtc cctcggcagg aaaaagatca acttcccaga
     4981 gcatcgcggt tcttttttcc tcgtagtaga atctgttcca gtgtgctttc atcaccggcg
     5041 gccaaggctc gggccagccg tcccagttcg tcggcaaagg cctggcacgc gccgacaaga
     5101 ttctcccggt tctgcatgca gatttgcttc cacatttcga catcacccga cgcaatgcgg
     5161 gttgtgtcga agaatccgct gccggtgagg cggaaccaag gttcgggcag catccccgcc
     5221 aaaagcgacg ccacaagatg gggaaaatgg ctggtcatgg ccaaggcctt gtcatgttct
     5281 tcgggcgaca tgcgaatgac caccgatccc aagccggtcc aaaaatcctc tagggcatcg
     5341 aagtcttctg cccgcgtgtt cttggtcggt gtgatgattg tcacccggcc ttcaaaaaga
     5401 ttgggatcgg agtagcttgg cccagtcttt tcgctcccgg ctaagggatg tgccccgaga
     5461 aaccggcagc cccgcggtaa cccctcatcc aaggcagcca cgatctggcc cttggcactg
     5521 cccacgtcgc tgatgagggc acgttcggga caggcctcgg ccacccgccg aaccagatcg
     5581 ggaattgtcc ccaccggcgt gcagacgata atcaggtcag cttgggcgac gccgcggtcc
     5641 agatcgatgg tcgtatggct gacggcgccc acacgccgcg cagcgcggag cgttgtttgc
     5701 cttcgtgcga tcccgatgac ttcatccgcc agaccgcgcc gcaggacggc ttgcccgatc
     5761 gaggccccaa tcaggcccac gccgacaatg gatatagttt tccagcgccg catgatcgca
     5821 ttccttccta gtgttcataa ggaaccaccc attttagcat atttgggcaa gtggtgacgc
     5881 ggggaccgcc cgttcgaact ctgcctcaaa ggacatcctt agtagacaaa aatcagcggt
     5941 tcgtccggag gcaccgggcc ttgccaccag gtgctaccgt gggtcaaatg ggtaacacat
     6001 gaggaaaagc gagaggctta tgatcctggg ctataacact aacggtctgg tccaccacga
     6061 acccacggca gccctccgcg gcttggcgaa gatcggttac cgtggggtgg ccctgacgat
     6121 tgaccactat ctcctttcgc ccttcaatct ctattgggag cggcaactcc acgaactacg
     6181 ggacgtgctc gatcgcctgg ggatgtactg cgtcatcgag acaggggccc gttttctcct
     6241 cgatccctgg cacaaacatg aaccaagctt gatctcccca gatgccagcg ggcgggtgcg
     6301 tcgccttgac ttctatcgcc acgccctccg ctgtgccgta acgcttcacg cggattgcct
     6361 ttctctttgg tcaggcaacc gacacgacga agtccctccc gagcaggcct gggagtggct
     6421 tatccagggc ttgagtcatc tatgtgacga ggcctccgca atgggtgttc ccgtggccct
     6481 ggaaccggaa cccggcatgt tcatcgctac cacggccgat tacgatgagc tttgccgtcg
     6541 cgccccgagt ttgggctgtt tgttgacgct ggatatcggc catgtactgt gccaggaaga
     6601 aggtcccatt tcctctattg tggaacagta cgccccgcgg attgtgaata tccacctcga
     6661 agacatgcgt cgcgggacgc atgaacatct catgtttggg gagggggaag tcgatctcgc
     6721 cgaggttttt gcttcccttc agaaaatcgg gtacaaaaaa gggatctacg tggaactgag
     6781 ccgacacagt caccttgggc tccaggcggc caaacaggcg tttgacattc tgtcccgtta
     6841 tcaatctggg atgacccagt gatgtcaccg gctgaagggt gctggtttgg gacatctcgg
     6901 caaagcatgt tacgttcctg aggcg
//
LOCUS       DAOPTR010000052         6788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_9296_length_6788_cov_3.659736, whole genome shotgun sequence.
ACCESSION   DAOPTR010000052 DAOPTR010000000
VERSION     DAOPTR010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6788
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9296_length_6788_cov_3.659736"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..782)
                     /locus_tag="PKI05_01795"
     CDS             complement(120..782)
                     /locus_tag="PKI05_01795"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOA50957.1"
                     /translation="MSRNLPRTALLAGYYEDYLKHQDTARFVAEVNVRYTQGTLQRLL
                     EHPACEIRRAAALALGFLGNYDSNAALGKLLNDPDPTVRLLAETSIRKVWLRYGTESQ
                     QKQLSVIIRLNAARRYQEAITRADRLIEEAAGFAEAYNQRAVAYYGIGEHAEAIRDCR
                     MALELNPYHFAAAAGMGQAYLELRDPVAALDAFRRALKLNPDLQAVRMQVARLERLIE
                     DA"
     gene            complement(1518..1859)
                     /locus_tag="PKI05_01800"
     CDS             complement(1518..1859)
                     /locus_tag="PKI05_01800"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOA50958.1"
                     /translation="MLKSKIHRATLTATNLDYEGSIAIDRNLMEAADLLPGEQVHVLN
                     INNGERLITYVIEAPAGSGTVMLNGAAARLGMKGDLVIILSYCSIDDATARQYTPKVV
                     RVDGQNRLLAG"
     gene            complement(2075..2557)
                     /locus_tag="PKI05_01805"
     CDS             complement(2075..2557)
                     /locus_tag="PKI05_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50959.1"
                     /translation="MSYQNTQYGKWYWMTPLVLGTLLVIDLLVPGQPIPWFGYAIAGG
                     TVAMVTLCFTSLTVRDGGDKLVVQFGPIPLVRATIPYRSITRVEQGRTNFFDGWGIHY
                     SLTGGTVLNIWGFDCVRIWCGSRLYSIGTDDPDGLLTFLKNKLADLGSNTGITEDGSK
                     "
     gene            complement(2609..3676)
                     /gene="recA"
                     /locus_tag="PKI05_01810"
     CDS             complement(2609..3676)
                     /gene="recA"
                     /locus_tag="PKI05_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOA50960.1"
                     /translation="MAKKAGLKPEEKEAKKDSSTPLIEKNPALKNAVQQIEKQFGEGA
                     IMPLGASKVEAIEGIPTGSLSLDLALGGQGIPRGRIIEIFGPESSGKTTLALHVIAQA
                     QKLGGIAAFIDAEHALDPAWAKRLGVDLENLLVSQPSSGEEAMHIAEMLIRSNAVDVI
                     VIDSVAALVPQKELEGEIGDSHVGLQARLMSQSLRKLTGAIAKAKTAVIFINQIREKI
                     GVMFGSPETTPGGRALKFYASCRIDVRRVGQIRDGEQVIGQRVKARVVKNKVAPPFRV
                     AEFDMLHDSGISYEGDVLDLAIERKLVVRSGAWFRYGDLQLGQGRERTREYLKEHPEL
                     VEELRQRILETPGTIHTEIAE"
     gene            complement(3906..4934)
                     /gene="truD"
                     /locus_tag="PKI05_01815"
     CDS             complement(3906..4934)
                     /gene="truD"
                     /locus_tag="PKI05_01815"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HOA50961.1"
                     /translation="MHAVSYGGLKDRHAITRQYLTIRNGPSSSLTLEGIRLEYLGQAS
                     RPFTSDDITANHFRIVIRDLTLEDMEKAITALQEVGQFGLPNYFDKQRFGSVGWSGDF
                     VARAWCLGDYERALWLALADPHDSDRSSDKKEKAYLREHWGDWLACKQVVRNRFRRAV
                     VAQLADQPGEYRRAMAALRRDQRGLYLAALQSYLWNVMASQWIVRHVPPENRFDVQIG
                     RWAFAFFRRLSDPVLEQFRTTHLPLPSARCPVKTGPIRELMEQAAAEVGLQVRQLRIK
                     YPRDSFFSKGDRPLLVFPERLVSHTERDELNAGRQKLTLEFDLPRGSYATILVKRITE
                     DHGETDLL"
     gene            4896..5195
                     /locus_tag="PKI05_01820"
     CDS             4896..5195
                     /locus_tag="PKI05_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50962.1"
                     /translation="MAILQSAVRDRVQGKSPLFADFIDGLRRAQRLLGQTIHRVGAVI
                     QGKISDFLDDKVSRYLFEFHEENTPLLIGKLVSFVRFVGTRGLTPVFAIFSQSVA"
     gene            5325..5834
                     /locus_tag="PKI05_01825"
     CDS             5325..5834
                     /locus_tag="PKI05_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOA50963.1"
                     /translation="MASVVQDSQEALIQELSHHGGSGSMQLVSFRLGQEEYGVEITKV
                     QEIILMGEITRVPQTPEYIKGLINLRSTVIPIVDLRLRFGLMPQEPTDETRIMVVNVR
                     GKTIGIIVDAVSEVLRIHRDQIAPPPPTVAGLGQKYLTGLVKLDKRLLILLDIERILG
                     DDDTDFGGM"
     gene            6143..>6788
                     /locus_tag="PKI05_01830"
     CDS             6143..>6788
                     /locus_tag="PKI05_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOA50964.1"
                     /translation="MLFSRSDKSPSSPSNRSKGVLGDSPGLVPKIVPIEPIVSPLNLP
                     QVTDIELQRYADLIYRRTGVRISPQKKTLLSNRLRRRLKETGIRTYSDYYEYLTKLPP
                     NHPEWDAFLQEITTHETYLFRDEVQWRWFREKFLPEMAATQAGPIKTLRIWSAACSTG
                     DEAFTAACCIAAALPDFRSWRITILGTDIGVAAVEQAKTAVFGERAMRLVPEDYK"
BASE COUNT         1546 a         1908 c         1852 g         1482 t
ORIGIN      
        1 gcggaagcca atccagtatc ttattatggc tggtcgaaag ccgaagcgga gcgacgggct
       61 gagaacgcca taagacgccg ggctgcctga tcgctcattg accaacctga ggtcgggcct
      121 caagcatctt cgatgaggcg ttctaatcgg gccacctgca tccgcacagc ctgaagatcg
      181 ggattaagct tcaaggcccg tcggaaggca tccaatgccg ccactgggtc gcgcagttcc
      241 agatacgctt ggcccatccc ggcagccgcg gcgaagtgat aagggttcaa ctcaagagcc
      301 atgcggcagt cccgaatagc ttccgcgtgt tcgcctatcc cgtagtaggc cactgcccgt
      361 tggttgtagg cctcggcaaa tccggcggct tcctcgatga gtcggtctgc ccgcgtaatg
      421 gcttcctgat aacggcgagc cgcattgagc cgaatgatca cactcagttg cttctgctgc
      481 gattccgtac cgtagcggag ccacaccttg cggatgcttg tctcggccaa gagccgaacg
      541 gtggggtcgg gatcgttgag caatttcccg agggcggcat tggaatcgta attgccgaga
      601 aagcccaggg cgagggcggc tgcccggcga atctcacaag ccggatgctc aagcaagcgc
      661 tggagcgtcc cctgcgtgta acggacattg acctcggcca caaaacgagc cgtatcttga
      721 tgcttcaggt aatcttcgta gtagcctgca agtaaggccg tgcgaggcaa attccgggac
      781 accgtcacac tcctgttgat caaaaacccg gcctcttcga cgcggccaac cgctcatcga
      841 gcgctccaga tcgcaaggac aagccacatt ccgttgccgt gcgtttggca cgcggacaag
      901 tcatttgatt cgattgtaat cagaccctgg ccaaacgctc aaacattttc catgggcaaa
      961 gcgctattgt ccacccggtg gcttggtcag gaacaggaaa aacaacccca cccgggaggg
     1021 ttcggccatg aggacacatt tgagaagaca cttgaggaag gaaacacggc ttgccggacg
     1081 aaactttaac cgcccaactt ggtgcgcaag gcgcgaggaa acactaggcg gagcgccgtt
     1141 gtgtcagacg attacttctt gcgatgccga atcttggacc gcattcggcg cttcttgctg
     1201 ccaatcttgc gacgcccttt gccagttttc tttgtgccca caggttactc caatttcaga
     1261 acaccaacat atgttcgaaa ataccaacaa cacctcggca tgaggaacca ttaaagggga
     1321 ggtcaaccac agatagcctt agtataactc tcagttagag gcggcgacaa ggatgggcac
     1381 caataccgcg ccgaaggtcc gcaccgtcat ggcgagagaa tgccccgtgc agtgatcagc
     1441 ttaatcgtcc gacgaaaatg aggtaatggg ccaagatatg aaacgattca cgcaaaaggc
     1501 cgacgatcgt tccccaatca tccggccaac aggcggttct ggccatctac ccggacaacc
     1561 ttgggcgtgt attgccgcgc cgtggcatcg tcgatgctgc aataagagag gatgataacc
     1621 aaatccccct tcatacccaa gcgggctgcg gcaccgttta acatgaccgt tccggaacct
     1681 gctggggcct caataacata ggtaataagt cgttccccgt tgttaatatt caagacatga
     1741 acctgctctc cgggcagcag gtccgctgct tccattaaat tacggtcgat agcgatactt
     1801 ccttcgtagt ccaaattcgt cgcggtaagc gtggcgcgat gaattttgga cttaagcatg
     1861 aatcgttgca cgccaaaacc cccatctaag atcacacagt cggcaaacct gcccgtacta
     1921 cttgggattc catctcaccc tgacgccaca taagcgggcg aagccatccc aacactttca
     1981 ttgtaggctt cttcccgaaa gaatgggcag gtcgccccga cgcgttctcg cagctctgcc
     2041 aaaaaatgac tcggtcaaac ctactagaaa aggctcattt tgagccatct tcagtgatac
     2101 ctgtattact tcccagatcg gccaatttgt tctttagaaa ggtaagcaaa ccatcggggt
     2161 catccgtacc gatgctgtaa agtcggctgc cacaccagat gcggacacaa tcgaatcccc
     2221 aaatgttgag caccgtaccc cccgttagac tgtagtgaat cccccagccg tcgaagaagt
     2281 tggttcgacc ttgttcgacg cgggtaatgg accggtaggg aattgttgcc cgaactaaag
     2341 gaattggtcc aaactgcaca accaatttgt ccccgccatc gcggaccgtc agcgatgtaa
     2401 aacatagcgt taccatcgcg actgtgccgc ccgcaatggc ataaccgaac caaggaatgg
     2461 gttgccctgg cacaaggaga tcgatgacca gaagagtccc aaggaccaag ggagtcatcc
     2521 agtaccactt gccgtattga gtgttttgat aactcataaa ggagaagtga ttccaatccc
     2581 ggtcagggac tccaaagatc gctggagatt actcggcgat ttccgtgtgg atggtccccg
     2641 gtgtctccaa gattcgctgc cgcagctctt ccacaagctc tgggtgctct ttgaggtatt
     2701 cgcgggtacg ctctcgccct tgaccaagtt gaaggtcacc ataccggaac cacgccccgc
     2761 tgcggacgac aagctttctc tcgatggcca ggtccagaac gtcaccctca taactaatgc
     2821 cggaatcgtg cagcatatcg aattctgcca cacggaacgg cggcgccacc ttgtttttga
     2881 cgacgcgggc tttgacacgt tgaccgatga cctgttcgcc gtcgcgaatt tgacccactc
     2941 ggcggacatc gatccggcac gaggcataga acttaagtgc ccggcccccg ggggtggttt
     3001 cgggcgatcc aaacatgacc ccgatttttt cccgaatttg attgataaaa ataacagcgg
     3061 tcttggcttt ggcgatcgcc cctgtcagct ttcgcaatga ttggctcatc agtcgggcct
     3121 gtaatccgac gtgagaatca ccgatttccc cttcaagctc cttttgagga accaaggcag
     3181 ccaccgagtc gatcacgatg acatcgacgg cgttagaacg gatgagcatc tccgcgatgt
     3241 gcatcgcttc ttcgccactg gatggctgac tgacgaggag attctcaaga tcaacgccca
     3301 accgcttggc ccaggccggg tcaagggcat gctctgcatc gatgaaagcg gcaattcccc
     3361 ccaacttttg cgcttgggcg ataacatgca aggcgagagt ggtctttccg cttgattcgg
     3421 gaccaaaaat ctcgatgatg cgtccgcggg ggattccctg cccaccgagt gccaaatcca
     3481 aggaaaggct gccagtggga atcccttcaa tggcctccac cttgctggcc cctaacggca
     3541 taatggcccc ctctccgaac tgcttttcga tttgctgaac cgcgttttta agtgccgggt
     3601 tcttctcgat aagaggggtc gaagagtcct ttttcgcttc cttttcttcg ggttttagcc
     3661 ccgctttctt ggccatgagg tcaacctccg gttgttcgct gtcaatcgtt ctcgataaag
     3721 aactgatctc atttccgcga tcagaagaag tctcggtaga ccggttctgc gacgattctt
     3781 ctcgtccgcg ctcagtatag cggtttgcgc ggcccagtca caagatacgg gtagcgaggc
     3841 cgcgcatcct ttgtgttttc tttctcgtca caaaatgggc tgccgctgag ggagagaccg
     3901 ctaccctaaa gcaggtctgt ttcgccgtga tcttctgtga tccgtttgac caaaatggtc
     3961 gcgtaggaac cccttggaag atcgaattcc agagttaact tttggcggcc agcgttcagc
     4021 tcgtcgcgct cggtgtggct aaccagtcgt tcaggaaaca cgagaagggg acgatcaccc
     4081 tttgaaaaga agctatcccg cgggtacttg atgcgcagtt ggcggacttg tagcccaact
     4141 tccgcggcgg cctgttccat cagttctcga atggggccgg ttttaacggg acaacgtgcg
     4201 gagggaagtg gtaaatgcgt ggttcggaat tgctccagca cagggtcgga gagccgacgg
     4261 aaaaaggcaa acgcccatcg cccgatttgg acgtcaaatc tgttctccgg ggggacatgg
     4321 cggacaatcc actgcgatgc catgacgttc cagagataac tttggagggc tgccaggtac
     4381 aatccccgtt gatcacgtcg caaggcggcc atggcccggc gatactcacc cggttggtcg
     4441 gccagttggg ccacgacagc ccggcggaat cggtttcgca ccacctgctt gcacgccagc
     4501 caatcacccc agtgttcccg caggtacgcc ttttcctttt tgtcactgga acgatccgag
     4561 tcgtgcgggt cggccagggc tagccacaat gcccgctcgt agtcgccaag acaccatgcc
     4621 ctggccacga aatcgccgga ccatcccacc gagccaaatc gttgcttatc gaagtagttg
     4681 ggtaaaccga attggccaac ctcctggagg gccgtgatgg ccttctccat atcctccaga
     4741 gtcaaatccc gaatcacgat ccggaagtgg ttggccgtga tatcatccga agtgaacggg
     4801 cgagaggctt ggcctagata ttccaaacga attccttcta aggtcagcga cgagcttggg
     4861 ccattgcgga tggtcaggta ctgccgtgtg atagcgtggc gatccttcaa tccgccgtaa
     4921 gagaccgcgt gcaggggaag tcgccactgt ttgcggattt catcgacggc ctccggcgtg
     4981 cccagcgatt acttggtcag acgatacacc gcgtaggggc cgttatccaa gggaaaatca
     5041 gtgatttcct cgacgataaa gtcagccggt atttgtttga gtttcatgag gaaaacacgc
     5101 cattgctgat tgggaagctc gtgtcgtttg tgcgttttgt agggacaagg ggtttaaccc
     5161 ctgtgtttgc gattttttct caaagtgttg cctgaagaaa ggcatcatgg gaatccataa
     5221 cctatagttt catcaaagcg gctcgtggga tattcgtcta gaagccgccg tttgaggtgc
     5281 aaaaaacggt tggaaaaatg ttcctgaacc aggaggtacc acccatggca agtgtcgttc
     5341 aagattctca agaggccctt atccaagagt tgtcccatca tggcggcagc ggcagcatgc
     5401 aactggttag cttccgtttg ggacaagagg aatacggcgt tgagatcacc aaggttcaag
     5461 agatcatcct catgggtgaa atcacccggg taccgcaaac gccggagtac atcaagggat
     5521 tgatcaatct tcgcagcacg gtgattccaa ttgtggattt acggctccgc tttgggttga
     5581 tgccccagga accgacggac gagacacgga ttatggtggt gaacgtccgc ggcaagacga
     5641 tcggtatcat cgttgatgca gtcagcgagg tccttcggat tcatcgagac cagatcgctc
     5701 ctccgccgcc gaccgttgcc ggcttgggcc agaaatacct gactggcttg gtcaaactgg
     5761 acaagcggct tctcattctg ttggacatcg aacgcattct tggcgacgac gacaccgatt
     5821 tcggtggaat gtaatcgcac tcgtagtgag gcgggacgcc tctaagccgc ctgatggttt
     5881 actcattgac tcggcccaca gccgcagccg ggaacgcccc accacagtga agggttgcag
     5941 aggccaagac agtccgccgc gctcaaaaga gcctctcggg gcgcagcggc tgtggcgccg
     6001 agagttgaag ctgcggatag gaacgctcgg tagcgttgcc actcggccgg actgcggtcg
     6061 gtggactcgg atggccaaac attcctggca tcagtttcca gtgtgcgagt gagttttgat
     6121 cccctgtgag gaatcgggaa cgatgctgtt ttctcggtcg gacaaaagcc caagctcacc
     6181 gtcaaatcga tccaaaggtg tgttgggaga ttcaccaggg ttggttccca agatcgtgcc
     6241 gattgagccg attgtcagtc ccctgaacct tccccaggta actgacatcg aactgcagcg
     6301 gtacgcggac ttaatctatc gccgcaccgg cgttcgtatc tcgccgcaga agaagacatt
     6361 actctccaac cgtctcagga ggcggctcaa agagaccgga atccgcacct atagcgatta
     6421 ttacgagtac ctcaccaaac ttcccccgaa tcacccagaa tgggacgcct tcttgcagga
     6481 aatcaccacc cacgaaacgt acctctttcg agatgaggtc cagtggcgat ggtttcgcga
     6541 aaaattcctg cccgaaatgg ctgccaccca agcgggacca atcaagacgt tgcgaatttg
     6601 gtcggcggct tgtagcacag gcgatgaggc cttcacggcc gcctgctgca tcgccgcagc
     6661 gctgcccgat ttccgttctt ggcggatcac cattttgggg acggatatcg gtgtcgcggc
     6721 cgtggagcag gccaagacgg ctgttttcgg cgaaagggcc atgcggctgg tccccgagga
     6781 ctacaaga
//
LOCUS       DAOPTR010000053         6759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_9347_length_6759_cov_3.466140, whole genome shotgun sequence.
ACCESSION   DAOPTR010000053 DAOPTR010000000
VERSION     DAOPTR010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9347_length_6759_cov_3.466140"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="PKI05_01835"
     CDS             <1..1209
                     /locus_tag="PKI05_01835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HOA50965.1"
                     /translation="ASVPCVQCGQCAAICPVGAISERDHVEAVWAALDTPENHVVVQT
                     APAIRAALGECFGYPPGTLVTGKMVAALRRLGFDAVFDTNFAADLTIMEEGTEFLMRL
                     RKAYVEGQQDVLPMFTSCSPGWVQYLEFFHHNLLPNLSTCRSPQQMFGAIAKTYYAKK
                     LDKRPEDIFVVSVMPCTAKKFEASRAEMNASGVRDVDAVLTTRELARMIRIAGIDFQS
                     LPDEEMDAPLGLCTGAGVIFGHTGGVMEAALRTVYEVLTGEDLPTPTLLATLDGIKEA
                     EITLKNVRPEWSFLEGVTLRVAVAHGLGNAEKLIRAIEAGERQYHFVEVMACPGGCIG
                     GGGQPRFTDNRVRLARIAAIRKEDAGKPLRKSHQNPQVKQLYEEFLGEPLSERSHKLL
                     HTHYIAREPV"
     gene            complement(1296..1670)
                     /locus_tag="PKI05_01840"
     CDS             complement(1296..1670)
                     /locus_tag="PKI05_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50966.1"
                     /translation="MRQRFLISSVCVVILTVSVPLVASAGENTFFGRRAAQRARSYSW
                     SGEYYGATWGAPIAVVVPPTAEFQTHWGWGVGNFRVTPIDHQFSRNWPGPAQGGYLPL
                     KPTPQWPTDTDQFGMYYIRGPW"
     gene            complement(1727..2077)
                     /locus_tag="PKI05_01845"
     CDS             complement(1727..2077)
                     /locus_tag="PKI05_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50967.1"
                     /translation="MQIRKLLGWKIGLFFFAAVVSWASTSPSMAGDGSFVMGRYQGSH
                     AGDLFPSYWAPAAPGGISAPLYVSPIPTPPHVGHVWVTYQPLYPHEFLYKHSKVYWRK
                     NPQGGWTRVVVGWY"
     gene            2349..4463
                     /locus_tag="PKI05_01850"
     CDS             2349..4463
                     /locus_tag="PKI05_01850"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HOA50968.1"
                     /translation="MVKSTEEMSLETSPEKRSGPQNGNGQTRSPRIRVTAESMAAKQR
                     EISVSEFFAKNRHLLGFDNPRKALLTMVKEAVDNALDACEEAGVLPEIWVRIDPTGEN
                     RYRVIVQDNGPGIVKKQIPLIFGKLLYGSKFHRLRQSRGQQGIGISAAGMYGLLTTGK
                     PVSIVSKTGPKKPAHFYQIQIDTKNNRPEIVNGRGEGIDIPPQEDIGQFLVPYGLPWS
                     DVPHGTLVAIEIEAKFQRGRGSVDEYLYQTAIANPHVTIHYVDPEGKSRDFSRVADVL
                     PPEPKEIKPHPYGVELGWLLTMLKDTKASTLSGFLVDSFSRISPAVAQRIAETAQLST
                     RANPRKISRHDAEALFRALQETKIQSPATDCLSPIGEELILKGLRELVPGEFYTAITR
                     PPAVYRGNPFQVEVGLAYGGAPATYRVSLEVLEELLAETDARTLRQFLTTTFDGIGPE
                     GADRIIHQARLKSRMSPAKLKAREREQLCQALQTVNLSEGQTMTILRYANRVPLLFQA
                     GACAITQTVMSTNWRPYGLTQSRNALPSGPVTLMVHFASVWVPFTSESKEAIAAYPEI
                     QRELRLAYQAVGRRLGLYLRRRLRVKQQGERRNIFLRYLKEVANAVAVIRNVDRESVY
                     RDLMEVAKRVTAEADLKLDEHGNPIEETPSSEEFDGAVIIVEQQQGNPPLRSGNSKPI
                     QLQLFEDNGENGEPSPQDEEAT"
     gene            complement(4634..>5109)
                     /locus_tag="PKI05_01855"
     CDS             complement(4634..>5109)
                     /locus_tag="PKI05_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HOA50969.1"
                     /translation="VTKILDCLYVGSCPTSQTDVQELKELGITAVLNLQTDEDIAWRN
                     IDRDGVSAAYRAYRLREIRYPIRDFDYEDLRRRLPEAVRLLHNLVKEGHRVYVHCTAG
                     MNRSPTVIIAYLHWAMNWDFPEAVQHVTRNHPCDPFLEAISEQSPPEWDEDLTGS"
     assembly_gap    5110..5119
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5267..6655)
                     /locus_tag="PKI05_01860"
     CDS             complement(5267..6655)
                     /locus_tag="PKI05_01860"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA50970.1"
                     /translation="MFQSEPRNHGKGRATNRRSFLKSAGTLGVGAATVLTGVQVFGES
                     SPSQQIVLGIVGCGGRGTSLATGFLERGDCRIAWVCDPFLPRAEALAKTVEQLQGARP
                     QVSDDMRRALDDKAVDAIISATPDHWHALSTVWACQAGKDVYVEKPTSHNAWEGRKMV
                     EAARKYQRVVQIGTQNRSAPYNWQAKEYISSGKLGRIHLCRIFNQKLWENFPVRPDSD
                     PPAGLNWDIWNGPAPEAKYNPNYQYHWHHFWRYSGGDIANDASHQIDLARWLLGVDYP
                     KTVYSVGGRFDSEGAAETPDTQIAVYEFDGLVVTFELTLYTPYMLKIDPEVRNSDMFP
                     LWQHCATRIEIYGSEGVMYVGRHGGGWQVFVRPKSREPVVAAQCYGRFPDPEHKANFV
                     DCIRTRKLPNADVEEGHRSALWIHFANISYRLGGVKLVIDPKAEQITNVPEAAALYRR
                     EYRPPYVIPDNV"
BASE COUNT         1545 a         1851 c         1883 g         1470 t
ORIGIN      
        1 gctagcgttc cctgcgtgca gtgtgggcaa tgcgcggcca tttgtcctgt gggagcgatt
       61 tccgagcgcg accacgtgga ggccgtttgg gcggcactcg acacgcctga aaatcacgtg
      121 gtggtacaaa cggccccggc catccgggca gcgttgggcg aatgctttgg ctatccgccg
      181 gggacgttgg tcacaggaaa gatggtggcg gccctgcggc ggttaggttt tgatgccgtg
      241 ttcgatacaa actttgcggc tgacctgacc atcatggagg aaggtacgga gttcttgatg
      301 cgtcttcgga aagcctatgt ggaggggcaa caggacgtcc ttcccatgtt cacaagctgt
      361 tctccgggat gggttcagta cttagagttt ttccatcata atttgctccc caatctttcc
      421 acctgtcggt ctcctcagca gatgttcggg gcaattgcca agacttacta cgcgaaaaag
      481 ctcgataaac gcccggaaga tatcttcgtc gtctccgtta tgccgtgcac ggccaagaaa
      541 ttcgaagcgt cacgtgcgga gatgaatgcc agcggcgttc gtgatgtgga tgccgttctc
      601 acgacacgcg agttggcccg catgatccga atcgccggga tcgattttca gagcttaccg
      661 gatgaggaaa tggacgcccc tttgggactg tgtaccggtg ccggcgtgat cttcgggcac
      721 acagggggag tgatggaagc ggccttacgc acggtttacg aagttctcac cggcgaagat
      781 ctaccgacac caacgctgct ggctacgttg gatggcatca aagaggcgga gatcactctt
      841 aagaatgtcc gccccgagtg gtcattcctc gagggtgtta ccttacgggt tgcggtcgcc
      901 catgggctgg ggaacgcgga aaagcttatc agggccattg aggctggtga acgtcaatat
      961 cacttcgtgg aggtgatggc ctgcccgggc ggctgtattg gcggtggtgg acaacccaga
     1021 ttcacggata accgtgtcag gttggcccga attgcagcca tccgcaaaga ggacgcgggg
     1081 aaaccgcttc gaaaatccca tcagaaccca caggtcaaac agttatacga ggagtttctg
     1141 ggcgagccgc tcagcgaacg gtcccacaaa ctcctgcata cgcactacat cgctcgtgag
     1201 cctgtctaac ccagcgcggt gatccccgtg gcgcagcact gtcaagacgc tcattttctg
     1261 ccgaagtggc ggaaaacagg cgattccgcc cgggattacc acggcccacg aatgtaatac
     1321 atgccgaact ggtcggtgtc ggttggccac tgcggcgtgg gctttaaggg gagataaccc
     1381 ccttgggcgg gtcccggcca gtttcggcta aactggtggt cgatgggggt gacacgaaaa
     1441 tttccaaccc cccagcccca gtgggtttga aactcggccg ttggcgggac aaccacagca
     1501 atgggggctc cccatgtggc gccgtaatac tcgccactcc atgagtaact tcgagcccgc
     1561 tgggctgccc gcctgccgaa aaaggtgttt tccccagccg aggcaaccag aggcacggag
     1621 acggtcagga taactacaca cacactgcta atcagaaatc gttgacgcat aggtacgtcc
     1681 tcacgtgaat cagtggcgtt ttctttttca acgatcaatc tgggatttag taccacccaa
     1741 cgacaaccct tgtccagcca ccttgggggt tcttacgcca gtaaaccttg ctgtgtttgt
     1801 acaagaattc atggggatac agtggctgat acgttaccca aacatgtccg acgtgcggtg
     1861 gggtggggat cggagatacg tagagggggg ccgaaatccc cccaggggct gctggcgccc
     1921 aatagctcgg aaagagatcc ccggcatgag agccctggta tctccccatg acaaagctac
     1981 catcgcccgc catgcttgga gaggtacttg cccagctaac aaccgcagca aagaaaaaca
     2041 agccgatttt ccagcctagc aatttccgga tctgcatatc cgtcatcctt ttgcaaataa
     2101 atcctcttcc gtccaacccg cgactatgcg cgcagatagg tgatgcaaca atctccttta
     2161 tcaactttcg gctacccttg acgacgaggg tgagtcttta taaaggcgaa gtaagaagca
     2221 agctttgcat aggcgatcta cgtcgtttga tctgtaggaa tcttcgggga taatcgctac
     2281 aatcgttaca ttcgtttcgt tcttccccta aaaaagtcgc aacagatagg agctgttttg
     2341 ccttggatat ggtgaagtcc acggaagaga tgtctttgga aacgagccca gaaaaacgat
     2401 ctggtcccca aaatggtaac ggacaaactc gctctccccg gattcgcgtg acggccgaat
     2461 cgatggccgc caagcaacgc gaaatttcgg tgagtgagtt ctttgccaag aatcgccact
     2521 tgctgggctt cgataatccc cgcaaggccc ttctcacgat ggttaaggag gcggtcgata
     2581 atgcccttga cgcctgcgaa gaggccggcg tcttgccaga gatttgggtg cgaattgacc
     2641 ccacgggcga gaaccgttat cgcgtcattg tgcaggacaa tggaccgggc atcgttaaga
     2701 aacaaattcc cctgatcttc ggcaaattac tttacgggtc gaagttccat cgtctccggc
     2761 aaagtcgcgg acagcaaggc atcgggatca gtgccgccgg aatgtatgga ctattgacca
     2821 caggtaagcc cgtcagcatt gtctcgaaga caggcccgaa aaagcctgcc catttttacc
     2881 aaattcaaat tgacacaaag aacaacaggc cggagattgt caacggccgg ggtgagggta
     2941 tcgacatccc acctcaagag gatattggtc aattccttgt tccgtacggc cttccttggt
     3001 cggatgtacc tcatggaaca ctggttgcca ttgagattga ggcaaagttt caacgcggtc
     3061 ggggcagcgt ggatgagtac ctttaccaga cggccattgc caatcctcat gtcaccattc
     3121 actatgtcga tcccgagggc aaatctcgcg acttttcacg ggtggctgac gtcttacctc
     3181 cggagccgaa ggaaatcaaa ccgcatcctt atggtgtgga gcttggttgg ctgttaacca
     3241 tgctcaagga cacgaaggcc agcacgttga gtgggtttct cgtggattcc ttttcccgga
     3301 tcagtccggc tgtagcccag cggatcgccg aaacggccca attgagtacg cgggccaatc
     3361 cccgcaaaat cagtcggcac gacgccgagg ccctctttcg cgcccttcag gagacaaaga
     3421 ttcagtcgcc cgctaccgac tgcctttccc cgattggcga agagttgatc ctcaaaggtc
     3481 tgagggaatt ggtccctggc gaattctaca cggcgatcac acgaccgccg gcggtatatc
     3541 gcggcaatcc ctttcaggtg gaagtgggcc tggcctacgg cggggcaccg gcgacctatc
     3601 gggtttcact ggaagttctt gaagaactct tggccgaaac cgacgcgcga acacttcgcc
     3661 agttcctgac aaccacattt gacggcatcg gaccagaggg cgctgatagg atcatccatc
     3721 aagcacgtct caaatcgagg atgtcgccgg caaagctcaa ggctagggaa agggaacagc
     3781 tttgccaggc tttgcaaaca gtgaacttga gtgaaggcca gacgatgact atcctccggt
     3841 acgcgaacag ggtgcctttg ctattccagg cgggggcgtg cgcgatcaca cagacagtca
     3901 tgagcaccaa ctggcggccg tatggtttaa cacaatcgcg gaatgccttg ccttctgggc
     3961 ctgtcacgct catggtccac ttcgcgagtg tgtgggtgcc cttcactagc gaatcgaaag
     4021 aagccatcgc ggcttatccc gagattcaga gggagttgcg acttgcctat caagctgtgg
     4081 gacgtcgcct cggcctatac ttgagacgtc gtcttcgcgt aaagcagcag ggagagcgtc
     4141 gaaatatctt tctccgctat ttgaaggagg tggcaaatgc tgtggccgtg atccgaaacg
     4201 tggaccgcga gtccgtttat cgggatctga tggaagtggc caaacgcgtc accgctgaag
     4261 cagatcttaa gttggatgaa cacggcaatc cgattgaaga aactccatcc tcggaggagt
     4321 ttgatggggc cgtgataatt gtcgaacagc aacagggtaa cccgccgtta cgatccggga
     4381 attcgaagcc cattcaactc cagctttttg aggacaacgg ggaaaatggc gaaccgtcgc
     4441 cccaggacga ggaagcgacg tagagactat ctcaaaaagt cgccctctcc ctccgggaga
     4501 gggttggggt gagggtaaac gaacatcgga atcgattgac aaaagcggca gcaatttgag
     4561 ccaccctcac ccccgacccg tctcccgccg aacgggcgag gggagattat cggataggct
     4621 cgtagtcaaa acgtcaagaa cctgtcagat cctcatccca ttcaggcggc gactgttcgc
     4681 tgatggcctc caaaaacggg tcgcaagggt ggtttcgggt tacgtgctga acggcctcgg
     4741 ggaagtccca attcattgcc caatggagat aggcaatgat gacggtgggc gatcggttca
     4801 ttccggccgt acagtgaacg tatacccgat gtccttcttt gacaaggttg tgcaacagtc
     4861 tgacggcttc tggtaatcgt cgccgtaggt cttcataatc aaaatcgcgg ataggatacc
     4921 tgatctcgcg caagcggtaa gctcggtatg ctgcggagac accgtctcgg tcaatgttgc
     4981 gccaggcgat atcttcatcc gtttgaaggt tcaatacggc cgtgatgccg agttccttca
     5041 actcctgaac gtccgtctgc gaggtggggc aagaacctac gtaaaggcaa tccagaatct
     5101 ttgtcacgtn nnnnnnnnng tgcatgatga atccgtattt gaggacgaca tacatctcct
     5161 gtagctgggg cacagtcttg ttaagctaca cccaagctac ttcaatatat cactgactta
     5221 acacaaccta ttccaaaagc agcccggata ctgtcaaagg gcccttctac acgttatcgg
     5281 ggattacata ggggggacgg tattctcgtc ggtaaagcgc cgctgcttct ggaacattgg
     5341 tgatttgttc cgctttgggg tcaattacga gcttaactcc tcccagccga taactgatgt
     5401 tcgcgaagtg aatccacagg gcactacgat gtccttcctc cacatcggcg ttgggcagtt
     5461 ttcgagtgcg tatgcaatca acgaagttag ccttatgttc cgggtcgggg aagcgaccat
     5521 agcactgggc ggccacaacg ggttcccggc tcttcggtct gacgaacacc tgccagccac
     5581 ccccgtgacg tcctacatac atcaccccct ctgaaccgta gatctcgatc cgggtggcac
     5641 agtgctgcca aagggggaac atgtcgctgt tgcgcacttc tggatcaatc tttaacatgt
     5701 acggtgtgta caacgtcagt tcgaacgtca cgactaatcc atcaaactcg taaacggcaa
     5761 tctgggtatc gggggtctcg gccgcgccct ccgagtcaaa gcgaccgccg accgagtaca
     5821 cggtcttagg ataatccacg ccaagtagcc agcgagccag gtcaatttga tggctcgcgt
     5881 cgttggcgat atcgcctccg gaatacctcc aaaaatggtg ccaatggtac tgatagttcg
     5941 ggttgtactt ggcctcgggg gctgggccat tccagatgtc ccaattcaac ccggccggag
     6001 gatcactatc gggacgcacg gggaaattct cccagagctt ttgattgaaa atgcgacaca
     6061 gatggatgcg tccgagctta cctgaggaga tgtattcttt cgcttgccag ttgtagggcg
     6121 cactgcggtt ctgcgtaccg atttggacaa cccgttggta cttcctggca gcctctacca
     6181 tcttacgacc ttcccaagca ttatgggatg ttggcttctc gacatagaca tccttgcccg
     6241 cctgacaagc ccacactgta ctaagggcgt gccagtgatc gggggtcgcc gagataatgg
     6301 catcgaccgc cttgtcgtcg agggcccgcc gcatatcgtc cgacacctgt ggccgcgccc
     6361 cctggagttg ctcgacggtc ttggccaggg cttcggcacg cggcaggaag ggatcacaaa
     6421 cccaggcgat gcggcaatcc ccgcgctcca gaaaaccggt ggctaaggac gtccctcgac
     6481 caccacaccc gacaattccc aagacgatct gttggctggg ggacgactcg ccaaagacct
     6541 ggacaccggt cagcactgtg gcagcaccca cacctaaagt gccggctgat ttcaggaaag
     6601 accggcgatt ggttgcgcgg cccttaccat ggttacgagg ttcgctttgg aacatagctc
     6661 gatctcctgt agaaaaccca ttgattcgtg gcatccccaa cgactggatt atctcaagaa
     6721 ccccgactga gggaaactac ttcttaccca tggcccgct
//
LOCUS       DAOPTR010000054         6687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_9479_length_6687_cov_3.438329, whole genome shotgun sequence.
ACCESSION   DAOPTR010000054 DAOPTR010000000
VERSION     DAOPTR010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6687
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9479_length_6687_cov_3.438329"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            723..1136
                     /locus_tag="PKI05_01865"
     CDS             723..1136
                     /locus_tag="PKI05_01865"
                     /inference="COORDINATES: protein motif:HMM:NF016156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HOA50971.1"
                     /translation="MAIPEQRRAVWQLWIASGAAVAWLTVVLAAVRDHLLRSWLSVPE
                     IRSFDLALQYQFYHSLGLIMCGLTARVLPSRAMNIVGFLFILGIVGFSGGLYLKIVIP
                     NLTIGWMIPVGATLWVIAWVLFAILAVIQRGENAS"
     gene            1582..1932
                     /locus_tag="PKI05_01870"
     CDS             1582..1932
                     /locus_tag="PKI05_01870"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HOA50972.1"
                     /translation="MELRIQTRRVNLDEATRELIERRIGFALDQFNGWVSSVDVTLED
                     VNGPRGGVDKQCRILASLRGGKHIKIEDQDADLITVINRAADRLSQVVAREIDRKRDR
                     KGAGCPETIIEETE"
     gene            2147..2671
                     /locus_tag="PKI05_01875"
     CDS             2147..2671
                     /locus_tag="PKI05_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl tetrahydropterin synthase family
                     protein"
                     /protein_id="HOA50973.1"
                     /translation="MDERFTVRVASPKLRFNAAHFVAFGSDSCEPIHGHDFRVSVTIE
                     GQLSPTGWIVDFVVLEEILHGIVADFDHALLIPEASPWVKVIKNADVIEITVGARSWR
                     FPSQDCRFLPIPNVTAELLTQYIGMRVREQLLSRAEGREGRLTTMTVELSEAEGCTAS
                     WSCALARKPDLQDS"
     gene            complement(2686..3918)
                     /locus_tag="PKI05_01880"
     CDS             complement(2686..3918)
                     /locus_tag="PKI05_01880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOA50974.1"
                     /translation="MSEAWSKKLPTNMNHEVCGRIVIRPDRVRPFLERHPWVRATSIE
                     LIEGAPPDGSVVDVVSRQGKFIARGIFNSRSQIRVRLYTWQDQPLDEPFWRHRLESAI
                     ALRRQLGLDTPNGATRLVFSESDGLSGLIIDKYRDFLVLQVNALGFQQRLETIVGILQ
                     ELLNPKGIIARTDMEIRDQEGITFESPVTWGNVPPGPILVQEHGLDVGVDLLSGQKTG
                     LYLDQRDNRLAAARLVKGKRVLDMFCYTGGFSLVASRCGQAKEVWGFDSSEKAIVMAR
                     ANAQQNGVTNTRFEVGDAFQTLKSLRQEEERFDVVILDPPKFASGKSTLPEAFRAYHY
                     LNRLAIDVLNPGGYLVTCSCSGYVTREDFFNVLFGVSQQVGRDIQILEQRGAAPDHPV
                     LVTCRETHYLKCFICRVL"
     gene            complement(3945..5294)
                     /locus_tag="PKI05_01885"
     CDS             complement(3945..5294)
                     /locus_tag="PKI05_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOA50975.1"
                     /translation="MEDWSKIPELAALDSRRHLVRIPPEVDVPLTERVRHLIDTPEFR
                     RLARISQLGLVSLVYPAAHHTRFEHSLGVYRLALLFLKHLFHDERFRVTIRRRDAERL
                     IVTALLHDVGHWPFCHPIEDMQLEGVPTHESFLWTFLEEGSLGDLLRTEWSIQPREIL
                     ELLNTPDNDARLRLLHSLLSGPIDIDKMDYLYRDSLHAGVPYGRFFDQQRLVASLCVN
                     AAGDGLAITEKGKTAAELMVFARYVMFSEVYWHHAVRSATAMFQRAFYDTYRELDLSK
                     VFRLTEWEFVETLRRATEGRPAWQLIEGLFGKSRVLYKRIVQYHYFDDPEIYGQLAGR
                     SYEWLVKCCCVLCDELSRVLGEQIAHHELLLDAPPKHREIEFHVDVCFPKEQVYRPLS
                     EVSPVVRALAQEQFDHYVKRVRLFGAPHVAPKVRGLAHLRDLILKAIEISNSRFGCQ"
     gene            complement(5607..>6687)
                     /locus_tag="PKI05_01890"
     CDS             complement(5607..>6687)
                     /locus_tag="PKI05_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOA50976.1"
                     /translation="GSLQFTPQYYTQVGKAPSTGCEPQITVTFRSNDVYVVSTKDLSN
                     VVLQFSNGTVQKFDGLKGKTGVFKGTGTFAGKRIVRCWVKSGCNASGEGPGYGERFED
                     TNENIKAYFGLNSVPYPYPVGSWDEYINYVKTNSFVNGAGYRKKYGYLTLVNYWLEQR
                     CQANETPSLWQVSEQPVRQLKEAVNVFFEYLSEVDTGDRVSLVVYNSANQTAITETPL
                     TRDFESIRTTVWRRQAGHYDHYTNIGAGIKNGVDELLARGRPNSFKMLVLMTDGKANR
                     PNDESTGRAYALQQAQRAAENKIAILTLALGLDADRALLEQIAEMTNGKSFVVTGSTE
                     ITDFRPELMESFRTIANHRPVRLVK"
BASE COUNT         1547 a         1768 c         1801 g         1571 t
ORIGIN      
        1 ccaagaccgc cgtgatccag ctgcaaatgc cataggagag gcccacgcca tgaagacggt
       61 tccaaatcgc agcccagatg gtaatgtgcc ctaagcacat tagggccaaa agactgaacg
      121 aaagataaac catccgttcc cttggttatt ttctataaca gacgcgcaac tactgaaaac
      181 gcagagtttg ccgaacgcca gatctcggga gaaacctgca aaccctgggc cagcgagtgt
      241 caaaaccgcg aaatcttcat tataccgaga aaacccgatc gcactacagc ggttgtattc
      301 tccggagttt tcccagttct cccagccacg gcaaagattt acgataacgc ttgcgaacac
      361 actcgttttc aaacgtggct tcgtgcgtcg aaatgcagac cttgcccatt ttgcgacttt
      421 gccatgactt tcatctattg tgccctattt tcacgacctg gattgtccaa aatgcccccg
      481 ggccacaaag actggacagc ctgtttgcac ggcgtgacgt tgcaaaaatg gatggtggtc
      541 caatgtcaag tttggagggc ctgcgtggtc ttcccggggg caatggtttt gttagatagg
      601 aacaacccgc gcggcttgtt agaatgacgc gatgcagctc agactcggca tctatcttca
      661 ttgatggtag cgttgggttg gcgtgtaacg aaaagactgt aaagggtgtg gagaccattc
      721 ccatggcgat accagaacaa cgacgagcgg tctggcagtt gtggatcgcc tcaggagcgg
      781 ctgttgcctg gctgacggta gtccttgccg ccgtgcggga tcacctcttg cgatcttggt
      841 taagtgtgcc ggaaattcgc tcgttcgatc tggcacttca gtatcagttt tatcattccc
      901 tcgggctcat catgtgtgga ttgaccgcaa gagtcttgcc gtctcgcgca atgaatatcg
      961 tggggtttct gttcattctg gggatcgtgg ggtttagcgg agggctctat cttaagatcg
     1021 tcataccgaa tctgacgatc ggatggatga ttccggtcgg agcgacgctg tgggttatcg
     1081 cttgggtcct atttgcaatc cttgctgtca tccaaagagg cgaaaacgca tcataagaca
     1141 cagcatcggc gcatatggtt cgcacggtcc cgtaacaaag cgcatgaacg aaacttctta
     1201 cgtcaagact agagctgttt ccccgtttta gggctagctc ccgagggttt gtagggtaaa
     1261 attaatagtg cgacgacgag ttgcttggag cacactcggg atggcaaaag ggaatggctc
     1321 cccttacggg gcttttggga taacggaatg aatgtgagcc cacaaattac cgtaagtctc
     1381 ggcgccgcca tcgtggcaaa gaaaggggat gccttcttac atgcgaataa cgtatggata
     1441 aaagggtctt cttgtcgtga atttcctaag tttgaacttt accgcgggtt gagttgggcg
     1501 cgcgggaaga tattgttaca gcaacttttc agcgggcttt tttggcacca cggtctcgca
     1561 gtttagccaa tggaggtcat catggaactg cgtatccaaa cccgtcgtgt gaatcttgac
     1621 gaggccacac gtgagttgat cgagcgtcgc attggatttg cgctcgacca gtttaatgga
     1681 tgggtttcaa gtgtggatgt cactctggaa gacgtgaacg gaccgcgggg tggggttgac
     1741 aagcaatgcc gcattttggc gtctttgcga ggcggtaagc atatcaagat cgaggatcaa
     1801 gatgctgact tgattactgt gattaatcga gccgccgacc gactcagcca ggttgtcgcc
     1861 cgcgaaattg atcgcaagcg tgatcggaaa ggcgcgggat gtccggaaac aatcattgag
     1921 gagaccgagt gattctcgcc cacatggtgc gggtcgcact tcatttgaag aaaccgcgag
     1981 accgcctgag cgacgggaaa tataggccaa aaaggggttg gcaatgattg tttccacttc
     2041 acgtggatgt ccgcgcgtct tctcggcggt caaccgatag gtgacgcgag gtcagtctga
     2101 ggatcgtcca ggttcaggag cgaggtccct gggtggagaa atgacggtgg atgagagatt
     2161 tacggtccgc gtggcctcgc ccaaacttcg gttcaacgcg gcgcattttg tcgcatttgg
     2221 ctcagactct tgcgagccaa ttcatggaca cgacttccgc gtaagcgtga cgattgaggg
     2281 gcaactctcg cccaccggtt ggattgttga ttttgttgtc ttggaagaga ttctccacgg
     2341 catcgtcgct gattttgacc acgccctttt gattcccgag gcgtcaccgt gggttaaagt
     2401 gatcaaaaat gctgacgtta tcgagatcac agttggtgcg cgctcctggc ggtttccgtc
     2461 ccaagattgt cgctttctgc ccatcccgaa tgtgacagcc gaacttttga cgcaatatat
     2521 tgggatgcgc gttcgagagc agttgctgtc ccgggccgaa ggccgcgagg gccgtctgac
     2581 cacaatgacg gttgaacttt ctgaggcgga gggttgcacg gcctcttgga gttgtgcctt
     2641 ggcccgaaag cctgaccttc aggactcgtg aacattggcg tgaagctaaa ggactcggca
     2701 aatgaagcac ttgagataat gtgtttcgcg acacgtcacc aggacggggt gatccggtgc
     2761 cgcaccacgc tgttcgagaa tctggatgtc gcgtcctacc tgctgagata ccccaaaaag
     2821 gacgttgaaa aagtcctccc gcgtgacata acccgagcaa ctacacgtga cgaggtaacc
     2881 tccagggttc agcacatcga ttgccagccg gttcaaatag tgataggcac gaaaggcttc
     2941 gggaagcgtg cttttgccgg aggcgaattt cgggggatcg aggatcacga catcgaagcg
     3001 ctcttcctct tggcgaagtg acttcaacgt ttggaatgcg tcgccgacct caaaccgcgt
     3061 atttgtcacc ccattctgtt gggcattggc acgcgccatg acgatggcct tctcactgct
     3121 gtcaaagccc cagacttcct tggcttgacc acatcgtgat gcgacaaggg aaaaaccacc
     3181 cgtatagcaa aacatatcca aaacgcgttt tcccttgacc aatcgggctg ccgccaatcg
     3241 gttgtctcgc tggtcgagat agagacctgt cttctggccg gacagcaaat ccacgcctac
     3301 atcaagccca tgctcctgaa cgaggatcgg acctggtggc acgttgcccc aggttaccgg
     3361 gctttcgaag gtaatgcctt cctgatcgcg aatctccata tccgtacggg caatgatgcc
     3421 tttggggttg agcaactcct gaagaatgcc gacgatggtt tccaatcgct gttgaaagcc
     3481 cagggcattg acctgcaaaa ccagaaagtc acggtacttg tcaatgatca atccacttag
     3541 cccgtcgctt tcgctgaaga ccaagcgtgt tgccccattt ggggtgtcaa ggcccaattg
     3601 ccttcttagt gcgatggcag actcgaggcg gtgtctccag aacggttcgt ccaagggctg
     3661 atcctgccat gtatagaggc gaacccggat ttggctgcga ctattaaaga tccctcgggc
     3721 gatgaatttg ccttggcgcg agacgacatc aacgaccgag ccgtctggcg gtgccccttc
     3781 gataagttcg atcgatgttg ctcgcaccca aggatggcgt tccagaaaag gtcgcacacg
     3841 gtctggtcgg atgacaatgc gtccgcaaac ttcatggttc atattggtcg gcagcttctt
     3901 actccaagct tcgctcatat gatctccttg ccccctccct gtcatcactg gcatccgaaa
     3961 cgcgaattgg atatctcgat ggccttgaga atcaaatcgc ggaggtgggc aagcccccgc
     4021 accttgggag caacgtgtgg tgccccgaag agccgaacac gtttgacgta atggtcaaat
     4081 tgttcctgcg ccaaggcccg cacaacggga gagacctcac tcaacggacg gtaaacctgc
     4141 tctttgggga aacacacgtc cacgtgaaat tcgatttccc gatgcttggg cggcgcatcg
     4201 agcaagagtt cgtggtgagc gatttgctcg ccgaggaccc ggctcagttc atcgcatagc
     4261 acacaacaac acttgaccag ccactcgtac gaacgaccgg ccagttgtcc gtagatttcg
     4321 ggatcatcga aataatggta ctgaacaatc ctcttataga ggacgcggct tttaccgaaa
     4381 agcccctcga tcagttgcca ggccggccgt ccctcggtag ctcgccgcag tgtctcgacg
     4441 aactcccact cggtaagacg gaaaaccttc gaaaggtcga gctcgcgata ggtatcgtag
     4501 aacgcccttt ggaacatggc tgtggccgag cgaaccgcat gatgccaata gacctcactg
     4561 aacatcacgt agcgagcgaa taccatcaac tctgccgcgg ttttcccctt ctcagtgatg
     4621 gccagaccat ctcccgcggc attcacacaa aggctggcaa ccaaccgctg ctggtcgaaa
     4681 aagcggccat agggcactcc cgcgtgtaag gagtcgcggt atagatagtc catcttgtcg
     4741 atgtcgatcg gccctgaaag caggctatga agaagacgca gtctagcgtc gttatcaggt
     4801 gtgtttagca gttcgagaat ctccctcggt tggatcgacc attcggtccg caacaaatcc
     4861 ccgagcgaac cttcctcgag aaaggtccat aggaacgact cgtgggtggg gacaccctca
     4921 agctgcatgt cttcgatggg atggcaaaaa ggccagtggc cgacatcgtg gagtaaggct
     4981 gtcacgatca gtctttcggc gtcgcggcgt ctgatggtga cacgaaaccg ctcgtcatga
     5041 aaaaggtgct taagaaacag gagggccagg cggtacaccc caagactgtg ttcaaatcgc
     5101 gtatgatgag cggccggata aacaagggag acaagcccca attggctaat tcgggcaagc
     5161 cggcgaaact cgggcgtatc aatcaagtga cggacgcgct ctgtcagcgg aacatcgacc
     5221 tctggcggaa tccgcacgag atgccgccgg gaatccaaag cagccaattc gggaatcttg
     5281 gaccagtctt ccataattgc ctatcagttt ttttgagagt ttgtcacaaa taacctcctt
     5341 gtcgtttaca catttaagga agcactttct gccatgctag atcacatatg gcaaactcgg
     5401 aaggggtcgt tgttgcttct tgtgggatgc gtggagaacc agagggaacg acgcggtgga
     5461 tgagaatttg ggcaaggcaa accacaaaaa gctgacctcc gctggagttc caacccacgc
     5521 agttggttca cagcggaggt tcgcgcctgt caacgcactt cgcgtcgatc tggtcctaga
     5581 ccgatggagg gtttacctcg tatttcctat ttaaccagcc gaacagggcg gtgatttgcg
     5641 atcgttcgaa agctctccat cagttccggt ctgaagtctg taatctctgt acttcccgta
     5701 acgacgaacg atttgccatt tgtcatctcg gcaatctgtt ctagcagagc ccgatcggcg
     5761 tccaagccca atgcaagggt caaaatggca atcttgttct ctgccgcacg ctgtgcctgc
     5821 tgcaaggcgt acgcccgccc ggtcgattcg tcattcggcc gattggcttt accatcggtc
     5881 atgagaacga gcatcttaaa actgttcgga cgaccccttg ctaggagctc gtcaacacca
     5941 ttcttgatcc ctgccccgat attggtgtaa tgatcatagt gacctgcctg acggcgccaa
     6001 accgtggtcc tgatcgactc gaagtctcga gtcaatggag tctctgtgat cgctgtttgg
     6061 ttggcggaat tatagaccac cagtgacacc cgatcacctg tatccacttc gctcaaatac
     6121 tcgaagaata catttacagc ctccttcagt tgcctgaccg gctgctcgct cacttgccac
     6181 agacttgggg tttcgttggc ctggcatcgt tgctccagcc agtagttgac tagcgtcaaa
     6241 taaccatact tcttgcgata gcctgctccg ttaacaaacg agtttgtctt gacgtaattg
     6301 atgtactcat cccaacttcc caccgggtag ggatagggca cgctgttaag cccaaaatag
     6361 gccttaatat tctcattggt atcctcaaag cgttccccat agccgggtcc ctcgccactt
     6421 gcgttgcacc ccgatttgac ccagcaccgg acgatgcgct tgcccgcgaa agtaccagtt
     6481 cccttgaaaa cgcctgtctt gccctttagg ccatcgaact tttggactgt tccattcgag
     6541 aactgcaaca caacatttga aaggtccttg gttgaaacca catacacatc attcgatcga
     6601 aatgtcaccg taatttgcgg ttcgcaacct gttgatggtg ccttacccac ctgcgtatag
     6661 tactgtggcg taaactggag tgaaccg
//
LOCUS       DAOPTR010000055         6679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_9493_length_6679_cov_3.404891, whole genome shotgun sequence.
ACCESSION   DAOPTR010000055 DAOPTR010000000
VERSION     DAOPTR010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6679
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9493_length_6679_cov_3.404891"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /gene="arsB"
                     /locus_tag="PKI05_01895"
     CDS             complement(<1..924)
                     /gene="arsB"
                     /locus_tag="PKI05_01895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOA50977.1"
                     /translation="MVNDLQHEDNRKDDVATTDVGAPAPTRKRLNFFERYLSVWVALC
                     MVAGVILGKSLPDLTATLRTWEFASGSQINIPIAVLIWLMIIPMMMKVDFASIRNVGR
                     RPKGLLITLFINWVVKPFSMALWAWLFFRYVFAAWIGTDDASQYIAGTIILAAAPCTA
                     MVFVWSYLTDGDPAYTLVQVSLNDLIMLVLFAPIVRFLVAGASSLHVPFQVLFYSVVV
                     FIVIPLSVGVILRRWFVSRRGIQWFEQELLPHLAPVSMAALLVTLILIFAFQADNITN
                     KFWHVVLIAVPILVQVYFNSSLTYWLMYLFRV"
     gene            complement(1737..2030)
                     /locus_tag="PKI05_01900"
     CDS             complement(1737..2030)
                     /locus_tag="PKI05_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOA50978.1"
                     /translation="MAQPHSLRPSDPATIPSELQELANAIQGLPEPYRAQLDPVFQRV
                     MDGIRRRRQILSLVQDALSELRMDMKYLLFDLEATRRERDEYRRQLEQLRPGE"
     gene            2383..3399
                     /locus_tag="PKI05_01905"
     CDS             2383..3399
                     /locus_tag="PKI05_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50979.1"
                     /translation="MDGHFPELFEKQRIRGRATGEMPFRLTVGLVLAFLWPFLTPAQT
                     TPPAPRWRYAGGDVAVRELSHRLETVAEVTTPVERITIEAGAGTAVYYLLPLSPAPLI
                     TEMKAAVALKSRSPGLQLFLSVVLPRARHPQTGEPLTVLVPGGQYRVPGEWEWLTVTN
                     IGPVLEREVRTLRLQLRRDIDVREAYADGLVLNLYGGPGTTDVLIGDVQLEGFLPLNR
                     GPQAASPYPESPGQSGGIGSNHGTPFNNDRISRGTLPLRPERTQRDWLNTPLGSGKEL
                     DWSVTPRSGADILPAQHLAPPPIEMVGSVLRVKGRPFFPRALEYHGEPLDLVRQLGFN
                     TIWI"
     gene            3448..5325
                     /locus_tag="PKI05_01910"
     CDS             3448..5325
                     /locus_tag="PKI05_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50980.1"
                     /translation="MVILRPDPKLFGLAQSGVQRTIGSSYDGVLAWDMGTQLSPQNVE
                     ELKGLAEQIRSVDPMANRPILAEPLTQLLPVSRAVDVLLIGRNPCFSDLELTDYGTWL
                     RSRPLLARPGTPVWVTVPTQPDPGVLSQWERWGLSADQLNRIPADQVRLLTYLALTAG
                     ARGLLFRSQSSLACPDEATRERCIALELLNRELEWIDPFLAAGQFLSMVPSDQREVIG
                     ALFRTDRARLLVPLWLGRRAQYVPGQSAADQVTFVIPGVPESNSAYLLLPNSLEPLEG
                     ERVAGGMRVKIKEFGPTSLIVLTQDPVVLGFLMDGARTAGPRIVQLERELAEMTFRQV
                     QKQHQVLMKYGPFVPQADSWFRMAAQEMAAADKSVGAGDYPAAFRHVHRAMRPVLLIR
                     RVDWEAFALSLDNIVAWPCAVSFDTLLAHLKWTDQLSEDRARPTLLPGGDFEAGGQIT
                     AMGWQPFQHAIPGIVAQAELSPSAARSGSFGLLISVRATQPQREQALVESPPIWIESP
                     PVAVQPGDWVCIEGWISIPRAITGSVDGLMIVDSLGGEVLARRMSQTVGWQPFRLYRR
                     AGNEGQVRITLVMTGLGEVCLDDLAVTVIPTDGPSQQPPVPDVQPLVPTLTQPLAGVG
                     R"
     gene            5653..5988
                     /locus_tag="PKI05_01915"
     CDS             5653..5988
                     /locus_tag="PKI05_01915"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOA50981.1"
                     /translation="MVKGIDPKTRARCDCRARVLKAMAHPSRLIMLEELAKSGPKCVC
                     ELAALVELDMSTASRHLSLMKEAGLLTDEKRGQMTYYHLRTPCVIKFLECVDGVIAEG
                     LRTQLSFLR"
     assembly_gap    6042..6051
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6103..>6679
                     /locus_tag="PKI05_01920"
     CDS             6103..>6679
                     /locus_tag="PKI05_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOA50982.1"
                     /translation="MSRKDWGILTILVVVFLLAYFLNFASPRIQNAVMEAFLMLQWYA
                     RNHTLACVVPAMFIAGGIATFLSKEGVLRHLGPKARKVEAYAVASVAGTVLAVCSCSV
                     LPMFAGIYRVGAGLGPAAAFLYSGPAINVMAIFLTARVLGFDLGLARVVGAITFAVII
                     GLLMAWFFRKEESDRVKAFMALPESPKPKRRL"
BASE COUNT         1469 a         1826 c         1910 g         1464 t
ORIGIN      
        1 tacccggaag agatacatga gccaatatgt cagcgacgaa ttgaaataca cctggaccaa
       61 gatgggcacg gcaatgagca caacgtgcca aaacttgttg gtaatgttat cggcctgaaa
      121 ggcaaagatg aggataagcg taacaagcaa ggcggccatg ctgacgggcg ccaaatgtgg
      181 cagcaattct tgctcgaacc actggatccc acggcgtgag acaaaccagc gtctcaaaat
      241 gacaccaacg cttaggggaa taacaatgaa cacgaccaca gaataaaaga gcacctgaaa
      301 cggaacatgg agtgaggaag cgcctgccac caggaatcgc acaatcgggg caaagagcac
      361 cagcataatc aggtcgttga gggacacttg caccagcgtg taggccggat cgccgtccgt
      421 caaatagctc cagacaaaga ccatcgccgt gcagggagcc gcggccaaaa taatggtccc
      481 ggcaatgtat tggctggcat catccgtccc gatccaggca gcaaacacat aacggaaaaa
      541 caaccacgcc caaagggcca tcgaaaacgg ttttaccacc caattgatga aaagcgtgat
      601 caaaagtccc ttcggccgac gacctacatt gcgaatcgag gcgaaatcga ccttcatcat
      661 catgggaata atcatgagcc aaatcagcac ggcaatcgga atgttgattt ggctgcccga
      721 agcgaactcc caggttcgca gtgttgccgt caagtcgggc aatgattttc ccaggatgac
      781 cccggcgacc atgcataggg ctacccacac actcaaatag cgttcaaaga aattgagacg
      841 tttccgggtc ggcgcagggg caccaacgtc cgtcgttgct acgtcgtctt ttctgttatc
      901 ctcatgctgt agatcgttta ccatggctac ctatccaact cgattgccga tgtttaagat
      961 ctatgcgttt gagcttacca aatccgcaag aaaatcaaaa aatgccagcg ctgttggttc
     1021 cgttttggag cgaccacggt ctctaaaacg acctgacaga ccacagcaag gcagtccttt
     1081 gggcagcaga ctcctcatcg acccgcagcc cgaggcttcc acctgatact cgagtcaggc
     1141 ctgaccatgc gtcgcctcga cgtggaatgt cgctcatatc catcctgtac gttctgagaa
     1201 ccgtgcacgc acaggacact gcctccggcg gcccgaaacc acggaagacc cgtgtagcca
     1261 cgtatgaccc agccgagacg cggggccgta aatcggtgat gttcgcgatc gccttacatt
     1321 tggccagttg gccaaacatg caatttattt tagcacagcc gaggattgcg ggcaagggtg
     1381 gaagtcgccg aacaaactaa gtgaacaagg gagaattagg gatggccact tttcatgtct
     1441 tctgtgagag aaacatcccg ttcatccttg agacggcgtg aacagtcctt cgtgtcgcgt
     1501 actgttttga cggcaatggc tatctcttgt gaaatcgatg ccaaagcgcg atggcgaaca
     1561 ttgtcggcaa gtgcacgaga tttggcactt caaaaggtct gcccaaaagt tataccgccc
     1621 ctttcgctga gcacgatgat aattgcccca ccacaaagac aaggcggtag tacagttggg
     1681 cctgcactac cgctgccgaa gaaggaggtg acgcgtatgt tacactcgga gaggtgttac
     1741 tcgccggggc ggagttgctc aagttgacga cggtattcgt cacgttcccg gcgggtggcc
     1801 tcaaggtcaa agaggaggta cttcatatcc atccgcagct cggatagcgc gtcctgaaca
     1861 agtgacagga tttggcgccg acgccttatc ccatccatca cgcgctggaa gaccggatca
     1921 agttgggcac gataaggctc aggcaaccct tgaatggcgt tggccaactc ctggagttcc
     1981 gagggaatcg ttgctgggtc actgggtcga agcgaatgcg gctgtgccat ggtgcgtctc
     2041 tcccttgggc ttgtgcagaa ggagagaaat gcatccgccg cgccgccgcc acgccaacga
     2101 gccacttttc ccttaagtta tttactgaaa atgacttaag gctatgactc gctttgtgcg
     2161 taaaacaaag cgtgttgccc cagcacaaca tttgctctgg caaaataccg caaatcgtga
     2221 taaaaacgta atttacgatt tagggacgtt tccgcgcatc tgttgcctaa agtcaacatc
     2281 ttgcttttgt tgccctgatt acctaggcga cgcagtctcg ctggagtaac cgatctgccg
     2341 gtggggaaat cggagaccgt cccttccaga aaagacggta ccatggatgg tcatttccca
     2401 gaactcttcg aaaaacagcg catccggggg cgggcgacgg gggagatgcc attccggctt
     2461 accgtaggat tggtccttgc ctttctttgg ccgttcttga cgccagcaca aaccactcct
     2521 ccggcgcccc ggtggaggta tgccggtggt gatgttgcgg ttcgggaact ctctcatcgg
     2581 ctagagactg ttgccgaggt taccacaccc gtggaaagaa tcaccatcga ggccggagct
     2641 ggtacggcgg tgtactacct cttgcctttg tcccctgcgc cgctcatcac cgaaatgaag
     2701 gcggctgtgg cgttgaagag ccgttccccc ggtttgcagc tatttttgtc ggtcgttcta
     2761 ccgcgtgccc gacatcccca aacaggtgaa cctctcacgg tgttggttcc cggtggccaa
     2821 tatcgcgtac ccggggaatg ggaatggcta acggttacga acattggacc ggtcttagag
     2881 agggaagttc gcacactccg tcttcaactg cggcgtgaca tcgatgtccg cgaggcctac
     2941 gctgatggtt tggtacttaa cctctatggt gggccaggga ccaccgacgt cttgattggc
     3001 gatgtgcaac tggaaggctt cttgccgctt aaccggggcc cacaggcggc gtcgccctat
     3061 cccgagtcgc cgggccaatc tgggggaatt gggtcaaacc atgggacgcc gtttaacaat
     3121 gaccggatct cccgcggtac tttgccactt aggccagagc ggacgcagcg agattggttg
     3181 aacacgccct tgggaagcgg taaagagctc gattggagcg tgacccctcg tagcggcgcg
     3241 gacatccttc cggcccaaca cttggccccg cctccgatcg aaatggtcgg ctcggtgctg
     3301 cgggtaaaag gccgcccgtt tttcccacgg gcgttggagt accatgggga accgttggac
     3361 ctcgtgcggc aactggggtt caacacaatc tggatctgag agccggtcga ggaacgcctt
     3421 cttcttgaag cgcggcaaca aggattgatg gtcattctgc gaccggatcc gaaactcttt
     3481 ggcttggcgc agagcggggt ccagcggacc atcggctcat cgtacgatgg cgttctcgct
     3541 tgggacatgg ggacgcaact ttcaccccaa aacgtcgaag aactgaaggg cttggccgaa
     3601 cagataagaa gcgtcgatcc catggctaat cgtccgattc tggccgaacc gttgacccaa
     3661 ttgctgcccg ttagtcgagc ggtcgatgtg cttttgatcg gccgaaaccc ctgcttttcc
     3721 gacttggaac tgaccgacta cgggacttgg ctccgcagcc gaccgttgct tgcccgacct
     3781 ggaacccctg tttgggtgac cgtccccaca caaccggacc ccggcgtgtt gtcgcagtgg
     3841 gagcggtggg gactgtcggc ggaccaactc aatcgaattc ctgcggacca ggtgcgtctg
     3901 ttgacctatc tggccttgac ggccggggcg agggggctat tgttccgctc ccaatcctcc
     3961 ctggcttgtc ccgatgaggc aacgcgggaa cggtgtatag ccttggagtt actcaatcgg
     4021 gaattggaat ggattgatcc gtttctcgca gccgggcagt tcctttccat ggtccccagt
     4081 gatcaacgag aagtgatcgg ggccctattc cgaaccgatc gtgcgcgact cctcgtacca
     4141 ctctggctgg gacggcgggc ccagtatgta ccagggcagt cggcggcgga tcaagtgact
     4201 tttgtcatcc cgggtgtgcc ggaaagcaat tcagcgtatc tgctacttcc caatagtttg
     4261 gaaccactgg aaggagaacg cgtcgcagga ggaatgcgcg tcaaaatcaa ggagtttggc
     4321 ccaacttctc tcatcgtcct gacccaggac cccgtggttc tggggttttt gatggacggg
     4381 gcacggaccg cgggaccacg cattgtccaa ttggaacgag aattggccga gatgaccttt
     4441 cgtcaggtgc agaagcagca tcaggtcctg atgaagtatg gtccattcgt tccccaggcc
     4501 gattcgtggt ttcgcatggc cgcccaagag atggccgctg ccgacaaatc ggtaggggcg
     4561 ggcgattatc cggctgcgtt tcggcatgtc catcgggcga tgcggcctgt cctgctcatc
     4621 cggcgggttg actgggaagc attcgcactg tctttggaca acatcgttgc ctggccctgc
     4681 gcggtcagtt ttgataccct actggcccac ctgaaatgga cagaccaatt gtccgaggat
     4741 cgggcaaggc cgacactgct cccaggcggt gatttcgagg caggtgggca gatcacggcc
     4801 atgggctggc agccgtttca acacgcgatc ccggggatcg ttgcccaggc ggaattgtca
     4861 ccatctgctg cccgttcggg gagctttggc ctactcatct cggtgcgggc gacgcaaccg
     4921 caacgcgaac aggccctggt cgagtcgccg ccgatctgga tcgaaagccc accggtagcc
     4981 gttcagccag gtgattgggt ctgcattgaa gggtggatct ctattcctcg agccatcacc
     5041 gggagcgtgg atggccttat gatcgttgat tccctcggcg gcgaggtcct ggctcgacga
     5101 atgagccaga ccgtgggttg gcaacccttt cgcctctacc ggcgggcagg aaatgagggc
     5161 caagtgcgaa tcaccttggt catgacggga ttgggcgagg tatgccttga tgatctcgcc
     5221 gtcacggtca tcccaaccga tggcccttcc caacaacctc ccgttcccga cgtccagccg
     5281 ctcgtcccta cgttaacgca acccttggcg ggcgttggcc gctaaatgca agatttcccc
     5341 tcaaaaccgc aaaagaacgt cgctaccgcc gtataggctg tcgttgggaa atgatcggca
     5401 ggctcggcgg acggcaggga acgacacagg gtcttggaaa cggcataaga caggctcgga
     5461 cgccatcctg cggggtcctg ggtaaccata caggggtcca gttcggtgtt gttgcaagaa
     5521 tttttgaacc ttttaccctt gtcgatcgat acaatggatg atacatttgc aaatatgtgc
     5581 aagaatgaat tgatgcaaga acgcgtttgt ctcaatcacg agatgtcaaa ctgacaaaag
     5641 aggaactcgg ttgtggtaaa gggaatcgat ccgaaaacgc gggcgaggtg cgattgccgg
     5701 gcacgggtgc tcaaggccat ggcccacccc tcgcgcctaa ttatgttgga agaattggcc
     5761 aagtcagggc caaaatgcgt ctgtgagctg gcggcgctgg tggaactgga tatgtccacc
     5821 gcctcccgtc atttgagtct catgaaagag gcgggcctcc taactgatga gaaacggggc
     5881 cagatgacct actaccatct gcggacgcct tgcgtgatca agttcttgga gtgcgttgac
     5941 ggcgtcatcg cagaaggcct ccggacgcaa ttgagttttt tgcggtaaag tgaggtattt
     6001 ggggtacctt tctcaagaac cacctccact ggtcgttctt tnnnnnnnnn ntgcatattt
     6061 ggccaagaat gcattaaatt aacggatcaa ggagttgacg caatgagtcg aaaagactgg
     6121 ggaattctta ccatcctcgt ggttgttttt cttttggcgt acttcctcaa ttttgcttcc
     6181 ccgcggattc agaacgccgt catggaagcg tttctcatgc ttcaatggta cgcccgaaac
     6241 cacacgcttg cctgtgttgt gccggcgatg ttcattgctg gtggaattgc gacgtttctt
     6301 tccaaggagg gcgtccttcg gcatctagga cccaaagcca gaaaagtcga ggcctacgcc
     6361 gtagcctcgg tagctggaac ggtcttggcc gtttgctcgt gcagtgtgct tcccatgttt
     6421 gcgggcattt accgtgtggg agccgggttg ggacctgccg cagcctttct ttactcggga
     6481 ccggcgatca atgtcatggc catctttctc acagcccgcg tcctcggttt tgacctgggt
     6541 cttgcacggg ttgtgggagc gataactttc gccgtgatca ttggcctttt gatggcctgg
     6601 ttcttccgta aagaggagag cgatcgggtc aaggccttca tggcactccc tgagtcgccg
     6661 aaaccgaaac gtcgtcttt
//
LOCUS       DAOPTR010000056         6578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_9659_length_6578_cov_3.279779, whole genome shotgun sequence.
ACCESSION   DAOPTR010000056 DAOPTR010000000
VERSION     DAOPTR010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6578
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9659_length_6578_cov_3.279779"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="PKI05_01925"
     CDS             complement(<1..964)
                     /locus_tag="PKI05_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOA50983.1"
                     /translation="MTFGAAAFLLAALAAIIPPILHMINRQRAKQLPFSTLRFLKISV
                     QKTRRRRQIRDLFLMLVRMAVLIFIAIGLARPTLSKLRNFMGGGQSAIAIVLDNSASM
                     GWIDEGRPRFEWAINAANQILNELQGGDEVTLWVTSGPRYPELGQVDRTHEKVRQLLG
                     QIAQRGPTFERGELGHLVREARYQLLQSKAANRYVFVITDNQAVAWEDLSMDEGTAEK
                     TADQEVEKSKDPHLRQIPIIIVDCQRNPKPNVAIRNVELSTILPVAGIPMKVIIELFN
                     SSSIPQQRVAELYMNGTKVGSSPEVSLAPQSTAVTEIVFTLDRGG"
     gene            complement(961..1860)
                     /locus_tag="PKI05_01930"
     CDS             complement(961..1860)
                     /locus_tag="PKI05_01930"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOA50984.1"
                     /translation="MSPQSAYRYVEPSALARVKNLQLVARGVVEGFISGLHASPYKGF
                     SVEFAEHREYVPGDDPRHLDYRMFARSERLYVKQYEEETNMRVQILLDVSGSMNYRYE
                     AKITKLEYGCYLTAILAYMMTRQQDSVGLTAFDDTIRLDMPARSSPRHFNEMMRQLEN
                     LQPGKTTGLAQVLHKLANRFKKRCLIILISDLYDDVDKVIRALHHFRHRKHEVIVFHV
                     FDKAELDFPFDDLVVFQDLETDEKIQVDPLYVREEYQKLLSEFVAEYRRACLESSIDY
                     VMTDTSVPYDFMLSRYLTKRNQL"
     gene            complement(1857..4103)
                     /locus_tag="PKI05_01935"
     CDS             complement(1857..4103)
                     /locus_tag="PKI05_01935"
                     /inference="COORDINATES: protein motif:HMM:NF018757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HOA50985.1"
                     /translation="MVEISLANGASIVSLVLVLAVAAGLVTVFYYRKFGELKRGRWQL
                     LLLLRLLAVVIVVLLLFRPVVHFYREERQKPTVVFLLDTSASMKIADDVSGIPRFTQA
                     RDKILLWAERLKEHFALQVIPFAERAGETIAISQLGTLSPDGPATSLSRALVAATKAA
                     PRSEMEAIFLVSDGIHNSARKPEEVSSRMQVPVFTIGVGTSLKTDASFKDIQITGLDC
                     PSRMMLNNLTRIKAFVEAVGLAGRVVKVVFLEDGQTIQEQELVLDDQPGPQEVMFEYR
                     PPVKGRHVYAVRIDPLSEEKIPENNERSAPALVVEAQIKVLYLEGTLRAEYGAIVDRF
                     LSKDPNIEFYALVQVKKNTFVKRTNMEGLTLESIPSDEATLNQFDVFIIGDLDSSFLS
                     SAQQEAIVNRIKNGGGLIMLGGYHSLGPGGYANTPIGQILPVELGDRDIGQLTDPFLP
                     ELTPDGRQHPIFANIARFFPTGGSSAEQQQLPPLDGCTRVGPARAAATVLAICPLTGE
                     RMPVLAVMPVENGRTAVFTGDTTRKWQQVPRVLDQESPFLQFWGQFVRWAAGRSEQLE
                     AKASVTAATDKAAYEPDEPIQLSAVVRDQRGEGTDKAKVTATIRAPSGAFDKVALVST
                     PGPGGHYSASYEPRFHGTLEIEVQAELEDEKLTAEKLIVEVGRPYLEFEKLDLDEKTL
                     TQIAADTGGRYAHISAADQLIDQLNQAERKKRIYVEQPLFWPPLFWAIFVALVTTEWV
                     LRRRFQLR"
     gene            complement(4157..4894)
                     /locus_tag="PKI05_01940"
     CDS             complement(4157..4894)
                     /locus_tag="PKI05_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50986.1"
                     /translation="MRPRCFSYVLTDRHIVQLPFISKIAGLMAAVIAVGFLWFGDLAT
                     ASEPAAINPFGPPVSEREDAVPGYVELSDGKIIAGKVYMTRDKRVKVFDSTLQRQREI
                     PLNRIKQIECIVVSERMEKEWRFRETTSNVKEYTGRSYPTREYLHRITLDDGRTIEGP
                     LSEVLYVQPEPSGSSSRGQQAAIEPQRFILYKTHKGPVGSDLKSLVYVKVVKLGEEAL
                     AEGQKKGSHSTSSRTTSQEASESKSRP"
     gene            5320..>6578
                     /locus_tag="PKI05_01945"
     CDS             5320..>6578
                     /locus_tag="PKI05_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50987.1"
                     /translation="MMSPAAEGVIRRLDATRQQWWFFTLLTSCVMAVSVSLATLLILV
                     LADALLRLSQPVLLGAFLVWMAVNLGLLVWVVRRVARSHRSLEATARRIEDQFPELQN
                     ALINVLQLLNDPRQCNPAFCLAAVNHAASQASRIRFEEAAARHTRWERFCYAIHTSRD
                     LLEWTGILVSLILLSVLCSLMLPNWGSAATRLMQPWHFVPVVGRVQILEVSPGNTEVL
                     VGSNVTISAKVRVPEKKPYDAFLYVIEDGKKEERFPMAVDKDLENYSFSLPSVLKPVK
                     YRVEVGDSQSPLYRLGVSEIPSIAEVEVTLDYPPYMEKPRDTFRQRVADLRAPQFSEA
                     TLKIIPSVPIAGGEITLDGQPLIGTTEDGGRAFLVKFPLLRDGEFAVKLRNMAGLTER
                     APRINRITVIPDQPPTVEIVKPTSQSSA"
BASE COUNT         1444 a         1772 c         1777 g         1585 t
ORIGIN      
        1 ttccaccgcg gtctaaggta aatacgatct ctgtgacggc cgtgctctgc ggggccaggg
       61 atacttcggg actacttccg actttcgtgc cattcatata aagttctgcc acccgttgtt
      121 gcggaatcga cgagctgtta aatagctcga tgatgacctt catgggaata ccagccacgg
      181 gcaggatcgt ggacagctcg acgttacgga tggccacatt cggtttcgga tttcgctggc
      241 aatcgacaat gatgatggga atctgtcgca aatgcgggtc tttggatttc tccacttctt
      301 gatccgcggt cttctctgcg gtgccctcgt ccattgacaa atcttcccag gccaccgctt
      361 ggttgtcggt gatgacaaag acgtaacggt tggcggcctt cgactggaga agctgataac
      421 gtgcctcgcg cacgaggtgt cccagttcac cccgttcgaa ggtggggccg cgctgcgcga
      481 tctgccccag taattgacga actttctcgt gagtgcgatc cacttggccc aattcggggt
      541 aacgagggcc acttgtaacc cagagggtga cttcgtcgcc gccttggagt tcattcaaaa
      601 tctgattggc ggcgttgatg gcccactcga atcgggggcg tccctcgtcg atccacccca
      661 tgcttgccga gttgtcgagg acaatcgcaa tcgcagattg cccacccccc ataaaattcc
      721 gaagtttact taaggtgggg cgggcaagtc caatcgcaat gaagatgagg accgccattc
      781 gcacgagcat aagaaacaga tcgcggattt gtcggcgccg ccttgtcttt tgaacactga
      841 tcttcaggaa acgaagtgta ctgaatggca actgcttggc ccgttgccgg ttgatcatgt
      901 gcagaattgg cggaatgatt gccgccaagg ctgccaacag aaatgccgcc gcaccgaatg
      961 tcataattgg ttacgtttcg tcaaataccg cgagagcatg aaatcatacg ggaccgatgt
     1021 atctgtcatc acgtagtcga tggaactctc caagcaagcg cgtcgatact ccgcgacgaa
     1081 ctcgctgagc aatttttgat attcttcgcg gacgtataaa ggatcaactt ggattttttc
     1141 atccgtctcc aagtcctgga aaacgaccag gtcatcgaaa gggaaatcaa gttccgcctt
     1201 gtcaaagaca tgaaacacaa tgacctcatg ttttcggtga cgaaagtgat gaagcgctcg
     1261 gatcaccttg tccacgtcgt cgtagaggtc gctgataagg ataataagac accgtttctt
     1321 gaaacgattg gcaagcttgt gtaatacctg ggccaggccg gtcgtcttgc ccggttgcaa
     1381 gttttcgagt tgccgcatca tctcattgaa atgccgaggc gagctgcggg ccggcatatc
     1441 aaggcgaatt gtgtcatcga aggccgtgag ccctaccgag tcctgttgcc gcgtcatcat
     1501 gtaggccagg atggccgtca aataacatcc gtactcgagc ttggtaattt tggcctcata
     1561 acgatagttc atcgatccac tcacatcgag caggatctga acacgcatgt tcgtttcttc
     1621 ttcgtattgc ttgacataaa gccgctcgga tcgagcgaac atgcggtaat cgagatgacg
     1681 ggggtcatcg ccgggcacat attcccggtg ctctgcaaat tccacgctga accccttgta
     1741 cgggctggcg tgcaagcccg agataaaccc ttccacgaca cctcgggcga caagctggag
     1801 attcttgacc cgtgctaggg ctgatggttc tacgtaacgg tacgcgcttt gcgggctcat
     1861 cttagctgga atctcctgcg gaggacccac tctgtggtta ccagagcgac aaaaatggcc
     1921 caaaatagag gcggccagaa caggggttgt tccacgtaaa tacgtttttt gcgctctgcc
     1981 tggttgagtt ggtcaattaa ttgatcagca gccgaaatgt gggcataccg accaccagta
     2041 tcggcggcaa tttgcgtcag tgttttttcg tcaaggtcca gcttttcaaa ttcgagatag
     2101 ggacggccaa cctcgacgat caatttttca gcggtgagct tctcatcctc tagctccgct
     2161 tgaacctcga tttccagtgt cccatggaat cgcggctcgt agctggccga atagtgacca
     2221 cctggtccag gcgtactgac cagcgcaacc ttatcgaaag caccggatgg ggcacggatt
     2281 gtcgccgtga ccttggcctt gtcggttcct tcgccccgtt ggtctctgac tacggcagag
     2341 agttgaatag gttcatccgg ctcataggcg gccttgtcgg tggctgctgt gacggacgcc
     2401 ttggcctcca attgttcgga ccgccctgcc gcccatcgca cgaattggcc ccaaaactgg
     2461 aggaatggcg actcctggtc gaggacccga gggacctgct gccattttcg cgtggtatca
     2521 cccgtaaaaa ccgcagttct cccattttct accggcatga cggccaggac gggcattctt
     2581 tcgcccgtca ggggacagat cgccaatacc gtggctgccg cccgggccgg tcccacccgc
     2641 gtgcagccat cgagtggcgg taactgctgc tgctcggcgg aactaccgcc agtggggaag
     2701 aaacgtgcga tattggcgaa aatagggtgc tgtcggccat cgggtgtcaa ttcgggcaag
     2761 aagggatcgg tcagttgtcc gatgtcccgg tcgcccaact ccacggggag gatttggccg
     2821 atgggcgtat tggcgtagcc acccggcccc aaactgtggt aaccgcctaa cataatgagc
     2881 ccgcccccat tctttatgcg attcacaatg gcctcctgtt gggcggagga aagaaaacta
     2941 ctatccaaat caccgataat aaaaacatcg aattgattta gcgtggcctc atcggagggg
     3001 atgctctcca gtgtaaggcc ttccatattt gtccgcttca caaaagtgtt tttcttcact
     3061 tgtaccaggg catagaactc gatgttgggg tctttggaga gaaagcgatc cacaatggca
     3121 ccgtattctg cgcgcagggt cccttccaag tagagaacct tgatttgggc ctcgacgacc
     3181 aacgcgggtg ccgagcgctc attgttttcg gggattttct cttcactcag cggatcgatg
     3241 cggaccgcgt aaacgtgccg tcccttcacc ggaggccgat attcgaacat gacctcttgc
     3301 gggccgggtt gatcatctag aacaagctcc tgctcctgaa tagtttgacc atcttccaaa
     3361 aaaacaactt tgaccactcg cccggccaga ccaactgctt ccacgaaagc cttgattcgg
     3421 gtgaggttat taagcatcat gcgtgacgga cagtccaggc ctgtgatttg aatatctttg
     3481 aaactggcgt ctgttttcag actggttcct acgccgatag tgaagacggg gacctgcatc
     3541 cgggaggaaa cttcctccgg ttttctggcg gaattgtgga ttccatcaga aacaagaaag
     3601 atggcctcca tttccgatcg gggcgctgct tttgtggcag cgacaagtgc ccgcgaaagc
     3661 gatgtggctg gaccatccgg agacaacgtc cccaattggc tgatggcgat ggtttcgccc
     3721 gcccgttccg caaaaggaat gacctgcagc gcaaaatgct ctttcaacct ttctgcccaa
     3781 aggaggattt tatctcgcgc ctgggtaaat cgaggaatcc ctgacacatc gtcagcgatc
     3841 ttcatggagg ccgatgtgtc caggaggaag acaacggtgg gcttttgtcg ctcctcgcgg
     3901 tagaagtgaa cgacagggcg aaagagaagc aaaaccacga tcacaacggc cagcagtcgc
     3961 agaaggagca ataactgcca gcgaccccgt ttcagttcgc caaatttccg gtaatagaaa
     4021 acggtgacta gtcccgctgc taccgcgagc actagtacca agctgacgat acttgcgcca
     4081 ttagctaggg agatttcgac cacagagaaa caggcacctc ttattgacaa ttcccgctag
     4141 gaacaacgga gcaaccttat gggcgactct tggactcgga cgcttcttgg gacgtcgtgc
     4201 gacttgatgt gctatgactg cccttcttct gtccctcggc aagagcttct tcccccagct
     4261 ttaccacctt cacataaacc agagacttaa ggtccgagcc aacagggcct ttgtgcgtct
     4321 tataaaggat gaaccgctgg ggttcaatgg cagcttgctg gccgcgtgag gatgatccgc
     4381 taggttcagg ctggacgtaa aggacctcgg aaagcggacc ttcaatcgtc ctgccgtcgt
     4441 cgagggtaat gcggtggaga tactctcggg tgggataact ccggcccgtg tattctttca
     4501 cgttggaagt tgtttccctg aaccgccatt ccttttccat ccgctcgctt acgacgatgc
     4561 actcgatttg cttgattctg ttgagaggga tttccctttg ccgctggagc gtgctatcaa
     4621 atactttgac gcgtttgtcc cgtgtcatat agaccttgcc ggcaatgatt ttcccatcag
     4681 agagctccac gtatcccggc acggcgtcct cccgctcgct gaccggcggt ccaaacggat
     4741 tgatagcggc tggctctgat gcggttgcta ggtccccaaa ccacaagaat ccaactgcga
     4801 tcactgcagc catgaggccc gcgatcttgc ttataaatgg taactgcaca atgtgacgat
     4861 ctgtcaaaac gtatgaaaaa cacctcggcc gcatcgtctc ggctcctttt tcaacattta
     4921 cccgtttccg caccagtgga aaccctcgca cgaacttttt cgaaccgagt ttcccaactt
     4981 ttcgttggct tacgaccagt tccaatcagt cttgcgacat cgagcgccat ttgaattagc
     5041 cgaacccttt tctattatcc attgttcatg atttaggacg attttccacg ggcatcattt
     5101 tgacaaattc cgcggcggga cctggaagcg atccctcttt gcttccaacc ccgagaaacg
     5161 gaatatcgaa cactttgaaa cctcggcaaa gaaaagaaat ggcgacgcag aatcccgata
     5221 aacagtgttg acgtcccccc taccggtggg aacaatgata agtgagaggt gatttcgcct
     5281 aaacttctca gtctttcgga aacagggaga ggacgcatca tgatgtcacc ggcggctgaa
     5341 ggagttattc gtcggctgga cgcgacgcgt cagcagtggt ggttttttac gttgttgacg
     5401 agctgcgtga tggcggtctc ggtttcactg gccaccctgc taatcttagt gttggcggat
     5461 gctttgcttc gtttgtcgca gcctgtgctt ttgggggctt tcctcgtttg gatggcggtg
     5521 aatcttgggc tgcttgtctg ggtcgttcgg cgtgtggcga gaagtcaccg cagtcttgag
     5581 gccaccgctc gccggattga agatcaattt cctgagcttc aaaatgcgct catcaatgtc
     5641 ttgcaacttc tcaacgatcc acgacaatgt aatcccgcct tttgcctggc agcggtcaat
     5701 catgcggctt cccaggccag ccggattcgc tttgaagagg cggctgcgcg gcatacccgt
     5761 tgggagcggt tttgctacgc gatacatacc tctcgggacc tattggagtg gacgggcatt
     5821 ttggtgagtt tgatcctcct cagcgtgcta tgtagcttga tgctcccgaa ctgggggtcc
     5881 gcagccaccc gattaatgca accatggcat tttgtgccgg ttgtggggag ggtccagatt
     5941 ctggaagtct cccccggaaa tactgaagtc cttgtcggaa gtaatgtgac gatttcggcc
     6001 aaggttcgtg tgccggagaa aaagccttac gatgcctttc tctatgtgat cgaagatggg
     6061 aagaaggaag agcgctttcc gatggctgtc gataaggatt tagaaaacta tagcttctcg
     6121 cttccctcgg ttttgaagcc agtgaagtac cgcgtggaag tgggtgacag tcagtccccg
     6181 ctctaccggt tgggcgtctc cgagataccg tcgatcgcag aagtcgaagt cactctggat
     6241 tatccgccct acatggaaaa accccgcgat acgtttcggc agcgagtggc cgatttgcgc
     6301 gcgccgcagt tttccgaggc gacgctgaaa atcattccct cggtcccgat cgcaggagga
     6361 gaaatcacgc ttgacggaca gccgttgatt gggaccaccg aagacggcgg tcgtgccttt
     6421 cttgtcaagt ttccgctact tcgcgacggc gagttcgccg taaagttacg aaacatggcc
     6481 ggtttaacgg agcgtgcgcc gcgcatcaat cgcattacgg tgattccgga ccagccgccg
     6541 actgtggaga ttgttaaacc aaccagccag tcttcggc
//
LOCUS       DAOPTR010000057         6545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_9717_length_6545_cov_2.808166, whole genome shotgun sequence.
ACCESSION   DAOPTR010000057 DAOPTR010000000
VERSION     DAOPTR010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6545
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9717_length_6545_cov_2.808166"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PKI05_01950"
     CDS             <1..234
                     /locus_tag="PKI05_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50988.1"
                     /translation="DWARRETGQCGVTGRSGSLPILLDRNKTQRAPEKIGNLPDALAL
                     GEMMQVGQPLSNLGCETRVKWAKNTGVKKGGLG"
     gene            313..1227
                     /locus_tag="PKI05_01955"
     CDS             313..1227
                     /locus_tag="PKI05_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50989.1"
                     /translation="MSVVERFNRLDRAVEDFQQAAEGFAELFQANEPRYEVYVSNRID
                     PKSGVTWDTMLNRLTDLLEASYKLLEAISDLPQKVQELLRRTEWPLLTMPQRAEEVIR
                     FAKCWIPLLEKRQSWEYYSFYYDFDGYVASFLGSVWEDWPQAMEDYQNRKAKIRRDCL
                     LSGEAAWFGDEAAEKSGATPATGMVSVNEATEKLQKGQTKGNGEAFIDRQEGPAEPEG
                     NMTEADALRSLLALFTQHVSDERFTAMAAILADPNRTIDEKLWEIHRRIPIPPTVDGP
                     TLASIFKTTKQAIYRTTWWQRNRRSSDD"
     gene            1424..1639
                     /locus_tag="PKI05_01960"
     CDS             1424..1639
                     /locus_tag="PKI05_01960"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOA50990.1"
                     /translation="MTGEREMLSLRETAKVLGLSERSVWTAIKAGRIPSTRIGKRVLI
                     PRRLLLERLEAEARAAMQASNDEGAGR"
     gene            1636..2568
                     /locus_tag="PKI05_01965"
     CDS             1636..2568
                     /locus_tag="PKI05_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50991.1"
                     /translation="MKPIDTVLTALDRHGCRPKRSGTGWSAQCPAHDDRRPSLSVTEG
                     TDGRVLVCCHAGCATESIVAALGLAMKDLMPDDVLQSHHVTTSPHHQNPVKRAKYVGC
                     ADARPGGNGKPDGQTFPAAEDALDSLRRKLGQESCRWTYCDAAGEPVGLVVRWDKPDG
                     TKDIRPISRFPDGWRIAAMPEPRPLYNLPDILKADPATPIFVAEGEKCVEALRQCGLL
                     ATTSSGGCNAASKTDWSPLSGRHVTILPDNDEPGRRYAQEVTDILLRLAPPATVRILK
                     LADYAPGLPEGGDVADIVESDGWFGLPLGQGASP"
     gene            2701..4437
                     /locus_tag="PKI05_01970"
     CDS             2701..4437
                     /locus_tag="PKI05_01970"
                     /inference="COORDINATES: protein motif:HMM:NF024547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HOA50992.1"
                     /translation="MRDWVQEEARATQTPLDLPAMMSLGVCSAAIARQVEVVPRVGWI
                     EPVNLFVTVLLPPGNRKSAVFRDALKPLRDYEAELIDMARPEIAKRQSARRQAEARLH
                     KLEKFAGEKDDQEAAEQACELAARLAVEPEPVLPRLLTDDATAEKIASMLAEQKGRLA
                     SMSPEGGPFDLMAGLYSKNQTPQFTVYLMAHAGDELIADRVSRKSVRVERPALTCCYV
                     IQPEVIRSLTHKAAFRGRGLLARFIYAVPVSTIGKREIAPPPVRSEVAEAYHRTVTML
                     ARQANAIGLAPLKLALTTEAELLLRTWEAEIEGMLAEFGEMETIRDWGAKLAGATLRV
                     AAVLHCVEQGIRGEIAPQTLAAAIEIGRYLIPHAKAVLEQLSDDSRLDGARWILRWIR
                     RHQLREFTLRDVYRHGYRHFDSVDECKAALMELERRGYVREQQLPPQGPSARNTPLYG
                     VNPVLYTPKGVSPIYGGEHVLEMPKGVDSADNVDTTPTTHDQSVNVVNVVSAFQKSEI
                     VLTPHKCGISNDEPQAGFHPTSGGANRHDGQAPSVPSGPPLAAPSHVVAVYGTQQEGG
                     EVDGPEFEEVTL"
     gene            4434..4901
                     /locus_tag="PKI05_01975"
     CDS             4434..4901
                     /locus_tag="PKI05_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50993.1"
                     /translation="MNPIALLKRPGVSVDWANGQLKLRFVKGGLTKDEIETLRQNKPL
                     VGGRLLLDKLWQAGYTLHLQRRSDGEGYFIVPRGQAHPGVDFPSLFELYDTFHDQAVA
                     LLLDMCRLGRIHPEDFNSREAWPIQFVDSNDQVLCETKGVATITGNKADKGRG"
     gene            4903..5616
                     /locus_tag="PKI05_01980"
     CDS             4903..5616
                     /locus_tag="PKI05_01980"
                     /inference="COORDINATES: protein motif:HMM:NF024502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase SAM-like domain-containing
                     protein"
                     /protein_id="HOA50994.1"
                     /translation="MASLSKHGHTFRLYVCLPDGRRVTLYLGKLPRRQAESFKVHVEQ
                     LAASMATATAIPGPTAVWLAALPDGLYEKLERCQLAPARVRETLGRLIERYEREYVAG
                     LALRSQSRIKNTTKLLSGYFGPKARLSAITQEDAERWRTWLAEGRTEATVRLHVRNAK
                     GLFNWAMTHRLADNNPFQTLVGASMARNDERYVSLEDFERLLDACPNHGWRVLLGLAR
                     LAGLRSPSETDSITWADIN"
     gene            5635..6012
                     /locus_tag="PKI05_01985"
     CDS             5635..6012
                     /locus_tag="PKI05_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOA50995.1"
                     /translation="MFAPKTKRTRLVPIVPRLRELLQQAYFAAHEGESLIVTVPKANR
                     HRTLRRIIERSGLTPWRDPFHCLRRSAATDFARSYPINAVAQWLGHGPVVSLKHYQRV
                     PDDLVRKATQDGFGDNRSLALLR"
BASE COUNT         1440 a         1840 c         1983 g         1282 t
ORIGIN      
        1 gactgggcgc ggcgagagac aggccagtgc ggagtgacgg gtcgcagtgg aagtttaccc
       61 attttgctag atcgcaacaa aacacagcgc gccccggaga aaataggcaa ccttcccgac
      121 gccctagcgc tgggggaaat gatgcaggtt ggacaaccct tgtccaacct ggggtgtgaa
      181 acccgggtga agtgggctaa aaatactggg gtaaaaaagg gcgggctagg ttaggccaac
      241 cgaactccgc tattgcccgg cggctgcccg ccctttgtct tattgggcgg tcacgttgtg
      301 cgagggcaac gcatgtctgt ggtggagcgg tttaatcggc ttgatcgggc ggtggaagat
      361 tttcagcagg cggcggaagg ctttgccgag ctgttccaag ccaacgaacc gcgatacgag
      421 gtatacgtga gcaacaggat cgaccccaaa agcggagtca cgtgggatac tatgcttaat
      481 cgcctgacgg acctgctgga ggcgtcgtac aagcttctcg aggcgatcag cgacctgccc
      541 cagaaggtcc aagagctcct gcgtcggacc gagtggccgt tactgactat gccccagagg
      601 gccgaggaag tgatccgctt tgccaagtgc tggatccctc tcctggaaaa gcgacaatcc
      661 tgggagtatt actcgttcta ctacgatttc gacggctatg tcgcgagttt tttgggaagc
      721 gtctgggaag actggcccca ggcgatggag gattatcaaa accgcaaggc gaagatccgt
      781 cgcgattgcc tactaagtgg ggaagcagcg tggtttggcg acgaggcggc agaaaagagc
      841 ggcgcaaccc cggcgacggg tatggtttct gtgaacgaag caacggaaaa gctgcaaaaa
      901 gggcaaacca agggtaatgg ggaggcattt attgatcgtc aggagggtcc cgcggagccg
      961 gaggggaata tgaccgaggc cgacgccctg cgaagccttc tggccctttt cacacaacac
     1021 gtgagcgacg agcgttttac ggccatggcg gccatcttgg ctgatccaaa caggaccatt
     1081 gacgagaaat tgtgggaaat tcaccggcga ataccaattc cgccgacagt tgacggcccc
     1141 acattggcat cgatattcaa gacgaccaaa caagccattt accgcaccac gtggtggcaa
     1201 aggaatcggc gctcgtctga cgactgaact tggcgggtaa cagttaccca aagttaccca
     1261 gcagttaccc gactcaaaaa atttggacgt tgcaaagccg cagaaaaacg ctgtctgcgg
     1321 cttttttcat gcgcttgcga ttacctaaac cgttacccgg cgccctcaag ggtagaaagt
     1381 cgaaaaaccc ttatgcacca cctgctccat gaaaggacca aacatgaccg gtgaacgtga
     1441 aatgctgtcg ttacgtgaga ccgcaaaggt tttaggcttg tctgagcgtt ctgtgtggac
     1501 ggcgattaaa gccggacgaa tccccagcac gcggattggc aagcgagttt taatcccgcg
     1561 gcggctgctg ctggaacggc tggaagccga ggcccgggcg gcaatgcaag ccagcaacga
     1621 tgagggggca ggccgatgaa gcccattgac accgttctca cggcccttga tcggcacgga
     1681 tgccgtccga agcggtcggg aaccggctgg tcggcccaat gcccggccca tgatgaccgc
     1741 cgaccgagcc tttcggttac cgaaggaacg gacggccggg ttttggtttg ctgtcatgcc
     1801 gggtgtgcca cggaaagcat tgtcgcggcc ctggggttgg cgatgaaaga cttgatgccc
     1861 gatgacgtgt tgcaatctca ccatgtcacc acatcgccac atcaccaaaa ccccgtaaaa
     1921 cgggcgaaat acgtaggttg tgctgatgcg cgaccgggcg gcaacggtaa acccgacggt
     1981 caaacgtttc ctgcggccga ggatgcgctt gattcattgc ggcgaaagct aggccaagaa
     2041 tcctgccggt ggacctattg cgacgccgca ggggagccgg tcgggctggt ggtgcgttgg
     2101 gacaaacccg acgggacaaa ggacatccgc ccaatcagtc ggtttcccga tggctggcga
     2161 attgcggcca tgcccgagcc acgtcccctg tacaacctgc ccgacattct caaagccgac
     2221 cccgcaacac caattttcgt tgccgaaggg gagaaatgcg tggaggcact gcggcaatgc
     2281 ggcttgctgg ccacgacatc cagcggcggc tgcaacgcgg caagcaaaac cgactggagc
     2341 cctctaagtg gccgacatgt taccatcttg ccggacaacg acgaacccgg gcggcgatac
     2401 gcccaggaag tgacggacat ccttttgagg cttgcgccac cggccacggt gcggattctc
     2461 aagctggccg actacgcccc gggactaccc gagggtggcg acgtagccga catcgtggaa
     2521 tccgacggtt ggtttgggct acccctcggc cagggcgcga gcccctagga ccttgggcgt
     2581 tggctgatcg aaatagcggc tggcatcgag ccggtcgcga gcaaagacga atggcccgag
     2641 gtaataagtc tcgaccctgg ggagctgccc cagttccctg tccacgtcct gcccacaata
     2701 ttgcgtgatt gggtccagga agaggcgcga gcaacacaaa cgccgctgga tcttccggcg
     2761 atgatgtcgt tgggcgtatg ctcggcggcc attgcacgcc aagtggaagt cgtccctcgt
     2821 gttggatgga tagagccggt caaccttttt gtcacggtgc tgctgccacc tggcaatcgc
     2881 aagtcggccg tttttcgaga tgctctcaaa ccgttgcgtg attacgaggc cgagctcatc
     2941 gacatggccc ggcccgaaat cgcaaagcgg caatctgcgc gaaggcaggc cgaagcgcgg
     3001 ttgcacaaac tggaaaaatt cgctggcgag aaagatgacc aggaagcggc cgagcaagcg
     3061 tgtgaactgg cggcccggct ggctgttgag ccggagccag tgctgccccg acttctcacc
     3121 gacgatgcga cagccgaaaa gatcgcttcc atgctggcgg agcaaaaggg ccggttagcc
     3181 agtatgtcgc ccgagggtgg cccatttgac ctgatggccg ggctgtacag caaaaaccaa
     3241 acaccccagt tcacggtcta tctgatggcc catgcgggcg atgagttgat cgctgatcgc
     3301 gtttctcgta agagtgttcg cgtggagcgg ccagcgctga cgtgctgcta cgtcatacaa
     3361 cccgaggtca ttcgcagcct gactcacaaa gcggcgtttc gtggccgggg gttattggca
     3421 aggttcattt atgcggtgcc ggtgagcacg atcgggaagc gggagatagc ccctccccct
     3481 gtgcggtccg aggtggccga agcctatcac cgcactgtga caatgctagc gaggcaggcc
     3541 aacgccatcg gccttgcccc attgaagcta gcactgacca cggaagccga gctgttgctg
     3601 cggacttggg aagctgaaat tgagggcatg cttgccgagt tcggcgaaat ggaaaccatc
     3661 cgcgattggg gtgccaagct agccggtgca acgctgcgcg tggcggctgt gcttcattgt
     3721 gtcgagcagg gcattcgtgg cgagattgcc ccacaaaccc tggctgctgc aatcgagatc
     3781 gggcggtacc tgattccgca tgccaaggcg gtgctcgaac agctaagcga cgattcccgg
     3841 ttggacggcg cacggtggat tttgcgctgg atccggcggc atcaattacg cgagtttacg
     3901 ctccgcgatg tttatcgcca cggctaccgt cactttgatt ccgtggacga gtgtaaggcc
     3961 gccctcatgg agctagagcg gcgtgggtac gtccgtgaac aacaattgcc cccccagggg
     4021 ccttcagccc gcaatacccc cctgtatggt gtcaaccccg tcttgtacac gccaaaagga
     4081 gtttccccca tttatggtgg cgagcacgtt ttggagatgc caaaaggcgt tgacagcgct
     4141 gacaatgttg acacaacccc aacgacgcac gaccaatctg tcaacgttgt caacgttgtc
     4201 agcgcatttc agaagtctga aatcgtgctc accccccata agtgtggaat atcgaacgac
     4261 gaaccccaag ccggttttca ccccacaagt ggcggggcta ataggcacga cgggcaagcc
     4321 ccctcggtgc cgagcggtcc gcccctggca gccccgtccc acgtcgtggc ggtttatggt
     4381 acgcaacaag aggggggcga agtggacggc cccgagttcg aggaagtaac cctatgaatc
     4441 cgatcgcatt acttaagcgg cccggtgtga gcgtcgattg ggctaacggc caattgaagt
     4501 tgcgtttcgt caagggcggt ttgacaaagg acgaaatcga aaccctccga cagaataagc
     4561 cccttgtcgg cggacggttg ctcctggaca agctttggca agcgggctat acgctgcatc
     4621 tccagcggcg gagcgatggt gagggctatt ttatcgtccc ccgcgggcaa gctcaccccg
     4681 gcgttgactt cccctcgtta ttcgagctgt acgacacatt tcacgaccaa gccgttgcct
     4741 tgctcctcga tatgtgccga ctagggagaa tccaccccga ggacttcaac agccgcgagg
     4801 cctggccaat tcagtttgtg gactccaacg accaagtgtt gtgtgaaaca aaaggtgtcg
     4861 ccacaattac cgggaacaaa gctgacaaag ggaggggctg acatggcatc actaagcaag
     4921 catggccata cattccgact gtatgtttgc ctgcccgatg gccggcgcgt gacgctctat
     4981 cttggcaagc taccacgtcg gcaagccgag agcttcaagg tccacgtgga gcaattggcc
     5041 gccagtatgg ccaccgccac ggcgattccc ggcccaacgg cggtgtggct ggcagccttg
     5101 ccggacggcc tttatgagaa gcttgaacgt tgccaactag cccccgcacg agtccgcgaa
     5161 accctcggac gcctgatcga acgctacgaa cgggaatacg ttgctggctt ggctttgaga
     5221 tcgcagtcgc gaatcaaaaa cacaaccaag ctactgagcg gttacttcgg ccccaaggcc
     5281 cggctgtcgg cgatcaccca agaggacgcg gaacgatggc ggacgtggct tgccgaagga
     5341 cgcacggaag cgaccgtccg acttcacgtt cgcaacgcca agggattgtt caattgggca
     5401 atgacgcacc ggctggccga caataaccca ttccaaacgc ttgtcggggc gtcgatggcc
     5461 cgcaacgatg aacgatacgt ctcgctcgaa gacttcgagc ggctactgga tgcgtgcccc
     5521 aatcacggct ggcgtgtgct actcgggctg gcccgacttg cgggcttgcg ttccccaagc
     5581 gaaaccgact ccatcacttg ggcagacatc aattaggaga ctgggacgct taaggtgttt
     5641 gcccccaaga cgaaacgaac ccggctcgtg ccgattgtcc cacgacttcg agagctactc
     5701 cagcaggctt actttgcggc ccacgagggt gaatcgctga tcgtcaccgt ccccaaggcg
     5761 aatcgtcacc ggacgctgcg gaggattatc gaacgctccg gcttaacccc gtggcgtgac
     5821 ccgttccatt gcctacggcg ttcggcagcc accgactttg cccgttccta cccgattaac
     5881 gcagtcgccc agtggcttgg ccatggcccc gtggttagct tgaagcacta ccagcgtgtg
     5941 cctgatgact tggttcgcaa ggccacccaa gacggctttg gcgataaccg cagtttagcg
     6001 cttttgcgat gaccacagtt tgacggcttt ggcgatgagc acagtttgac gctcttgcga
     6061 tgagcacagt ttagcgcttt ggcgatcagc atagtttgac gcttttgcga tgagcacagt
     6121 ttagcccaaa gttcctggat gtatttttac gtaagtcatt gctgggtaac gagttcaggt
     6181 tgatttgtgt tgcatactgt agtcatgcga tggttttgcg taagttcttg tcctgctttg
     6241 agttgcgcga tgtgcgccgt acgcagaaaa cggtggggga atcagggtgc cctgagctgg
     6301 gccgatgggg actcgactta gctgtacaca gaaaacgatg cggaatcagg gtgcttcctg
     6361 atggtagatt ccacggacca cgtgggtgta cacagaaaac ggtgggggaa tcagggtgct
     6421 taatgcgcgc aaatcattgt cttgaaatca cttacagtca tcatcccccc agttagcagg
     6481 gggaagtttg gcttgaagca ctaccagcgt gtgcctgatg acttggttcg caaggccacc
     6541 caaga
//
LOCUS       DAOPTR010000058         6474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_9834_length_6474_cov_3.738744, whole genome shotgun sequence.
ACCESSION   DAOPTR010000058 DAOPTR010000000
VERSION     DAOPTR010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6474
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9834_length_6474_cov_3.738744"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PKI05_01990"
     CDS             <1..645
                     /locus_tag="PKI05_01990"
                     /inference="COORDINATES: protein motif:HMM:NF021645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HOA50996.1"
                     /translation="WGGYLTCIVAGVDHRFSLAVPVYGCGFLHENSVWLPRFEKMTPE
                     QRERWVRFFDPSNYLGRVKCPIFFLNGTNDFAYPLDSYRKSYDRVPGEKLIRIEVRMK
                     HSHPDGWAPQEIRLFADSLLKGGMPLPKLTPLRRDGRRVWADVTSQTALREAHLNYTL
                     DAGRWQERQWVTVPARLEGQRIVAELPEPQPTAFYLDVVDERGAMVSTPPEILR"
     gene            840..1763
                     /locus_tag="PKI05_01995"
     CDS             840..1763
                     /locus_tag="PKI05_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOA50997.1"
                     /translation="MIDFDVFSRHSRRDILKGLLAGGAMILGIKGFGHMGFFAEEVTG
                     AAQALEKLGWTVACQLYTFRRFPFYEAIEKIAALGIKAVEPCFFLALDKGQPGLVTSE
                     ALTPEKRAEMKQRLARLGMTMPNFYANVGGDAEQAKKVFAFAKEMGCQTIVAEPPPEA
                     LPMLDELLAEYGLRLAIHNHPKSPQSRYWHPKNVLEAVRDRSERIGACCDTGHWVRSG
                     LDPVECLKMMEGRVITLHLKDVAEVGVVEARDVPLGTGAAKYDLVLQELARQKFRGVL
                     PIEYEHDSPQLEEDVAACRDFVLGWAAKNVS"
     gene            1824..3440
                     /locus_tag="PKI05_02000"
     CDS             1824..3440
                     /locus_tag="PKI05_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOA50998.1"
                     /translation="MACHRSPAPSVFKHWFIGFVIGVSCLLARGQRPFGAMEGFIVFA
                     AEDNPQFSKLWGREGELWTPQSRLPDFSYAGYRWGQESYRIPKESISVKDFGAKGDGQ
                     SDDTAAFKRALAAGKGKVIVIPPGRYILRDILTISSSNTVLRGAGPGKTFLVFTKPGI
                     EIDPRPSKTDGNQPTTNWSWAGGLISIGGRRQQPRDVVKVVGSQKRGDTQLSVEKNPF
                     RPGDEILLTLRDDEKNSLLTYLYRGQPGDISGLRNWSVEQVFRIVSVEGSTLKLNRPL
                     RFDIRSEWQPTVERFLPPLADVGVEGITFDFPVQQYAGHFKEIGFNPVEITRSAAHCW
                     LKDLVIRNADNGPFVSGYFCTVEKIRFEADPQRVSSSGNYTGHHGVTFLRCSDCLCRD
                     FEFNTRFIHDLTVQSSIGCVFCSGRGIDICFDHHRWAPYENLFTDIDAGTGKRLFASS
                     GGGMRGNHTAGGETFWCIRTQGLVRWPKSLGIDAINVVGLNVSDAAEAVPSLPSPTEL
                     TGRWCEPISPEVLQPQNLYDAMRKRVASPQ"
     gene            3941..4201
                     /locus_tag="PKI05_02005"
     CDS             3941..4201
                     /locus_tag="PKI05_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA50999.1"
                     /translation="MTRLFSQRRRRPSRRGILSFEWILLISILVIGIIGGLAAVRNAL
                     LSELNDLSQAVEAINVCGTCDPASCPPGNPDCCDDPWWCCGP"
     gene            4383..5087
                     /locus_tag="PKI05_02010"
     CDS             4383..5087
                     /locus_tag="PKI05_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51000.1"
                     /translation="MLPRLAVGCFFLLIAFLFRAFWLPVVAYPLMAPAPQHENAVTLI
                     WARSSDGKTPSGDGIVERTASLLKTHPHARVIFTQGWSSRLVELGVIPPPYETFRSAL
                     AKYQIESERVINLEVNGFEFWNEAKAVDAFLREHPEERVLLLVEEFAGQSHRYVLRRA
                     LDVSHRDRVEILGLPDLQYSYHDWWRSRSGVKGVMVGYLFLIHTILQPQPLRPPRKLS
                     LEDFEQSIPEAPGGRP"
     gene            5084..5812
                     /locus_tag="PKI05_02015"
     CDS             5084..5812
                     /locus_tag="PKI05_02015"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOA51001.1"
                     /translation="MKRVWWLLACLILSGLAILFHGRWLPSLAKYLDVGEAPRKSDYI
                     MIMGGGVENRPFTAAALFKKGWGERILIAELRPTPGGENLLPPEHEVTSRLLQKLGVP
                     EDRIIVLRGNHAATFHEIVTLGELLAREPEARVMIVTDPVHTRRTRWSVQRILGERAS
                     RISFVSAPSDGYNPDDWWQTGQGFFMVTSEYLKLLFYAFRYGWGLHLTAAAVALILLI
                     GILARYLRPMGRDKASTREAVPTS"
     gene            6100..>6474
                     /locus_tag="PKI05_02020"
     CDS             6100..>6474
                     /locus_tag="PKI05_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOA51002.1"
                     /translation="MTESSHTFSSEKKIRVFIADDHDMVRIGLKAMLSVPDIEVVGEA
                     GTGEDAVNFLLNNDVDVAILDVRMPRGDGLNALGRIKLEKPELPVLMLSTYDNPTYIA
                     RAVAFQAQGYLLKSCSREELLDA"
BASE COUNT         1295 a         1790 c         1924 g         1465 t
ORIGIN      
        1 tggggtggat acctcacgtg tattgtggca ggggtggatc atcgtttctc attggccgtc
       61 cccgtttatg gctgcggttt tctccacgag aacagcgtgt ggcttccccg cttcgagaag
      121 atgacgccgg agcagcgcga gcggtgggtc cgcttttttg atccttccaa ctatctaggc
      181 cgggtcaaat gccccatttt cttcctcaat gggacgaacg attttgccta tcctctggat
      241 agctaccgca agtcgtatga ccgcgttccc ggtgagaaac tcatccgcat cgaagtccgc
      301 atgaagcaca gccaccccga cggttgggcc ccgcaagaga ttcgcttgtt cgccgacagc
      361 ctgctgaaag gtgggatgcc tttgccaaag ctgacgccgc tccggcggga tggaagacgg
      421 gtctgggccg atgtgacatc tcagacggcc ctccgtgagg ctcatctcaa ttacacgctc
      481 gatgcgggac gctggcagga acggcagtgg gtaaccgtcc ctgcacgcct cgagggtcaa
      541 aggattgtcg ccgagttgcc agaaccgcaa cccacggcgt tttacttaga tgtggtggat
      601 gaacgcggtg ccatggtttc cacgccgcca gagatcctcc gctgatctta cggatataag
      661 actgcgcgac acgctctggg ttgcaatgga gggtcacgcg ccgcctcggc cgcaaagacg
      721 cccacgattg cctttgcaca agcggtgact actggttttc cgcatccgaa ctgactatcc
      781 tagtgtcgcg ggtattggca gagattttcg cgacaacttc acgggaacaa ggtgacgcca
      841 tgatcgattt tgatgttttc tcacgccatt cacgacggga tatcctgaag ggtctgctgg
      901 caggtggtgc gatgatactg ggaataaagg gcttcggaca catggggttt ttcgccgaag
      961 aggtgaccgg cgccgcgcag gccctcgaaa agctcggctg gacggtggct tgccagcttt
     1021 acacatttcg ccggtttcct ttctatgagg ccattgagaa gatcgccgcc ctgggcatta
     1081 aggccgtcga gccgtgcttt ttcctggcgt tggacaaggg tcagccgggg ttggtgacga
     1141 gtgaggccct cactccggaa aagcgggccg agatgaaaca gcggctggcc aggttgggaa
     1201 tgaccatgcc gaacttttat gcgaacgtcg gtggcgacgc cgagcaggcc aaaaaggtct
     1261 tcgccttcgc caaggaaatg ggatgtcaaa ccatcgtggc cgaacctccg cctgaggccc
     1321 tccccatgct ggatgaactg ctcgcggaat acggtctgcg tctggccatc cacaaccatc
     1381 ctaagtcgcc gcagtcgcgc tactggcacc ccaagaatgt tttggaggcg gtccgggacc
     1441 gtagcgagcg aatcggcgct tgttgcgaca cggggcattg ggttcgttcg ggattggacc
     1501 cggtggagtg tttgaagatg atggaagggc gtgtgatcac cttgcatttg aaagacgtgg
     1561 ccgaggtcgg cgtcgtcgaa gcccgcgatg tcccgctggg taccggcgcc gccaagtatg
     1621 atcttgtatt gcaagagctc gcccggcaaa agttccgtgg cgtgctgccc attgaatacg
     1681 agcacgactc gccccagttg gaagaagatg tcgctgcctg ccgggacttc gtcctcgggt
     1741 gggctgcaaa aaatgtgtct tgatcgaacg atagtgtggt tcctcccgaa agtaaagttt
     1801 tcccgccttg aaggagccga acgatggcct gccaccgtag ccctgcgccc agcgtattca
     1861 agcattggtt catcggattt gtgatcggtg taagctgcct cctggccaga ggccaaaggc
     1921 cgtttggtgc gatggagggc tttatcgttt tcgcggcgga agataatccg cagttttcaa
     1981 aactttgggg acgcgaaggg gagttgtgga caccccagag ccgtttgccc gacttttctt
     2041 acgccggtta ccggtgggga caagaatcgt accgcattcc caaggaaagc atctcggtga
     2101 aggactttgg ggccaagggc gatggccaaa gcgacgacac ggcggcgttc aaacgtgcgc
     2161 ttgccgcggg aaagggcaag gtgattgtca ttcccccggg gcggtatatc ttgcgagata
     2221 ttctgacgat cagtagctca aataccgtct tgcggggtgc cgggccgggc aagacctttc
     2281 tcgtttttac caagccgggg atcgaaatcg atccgcgacc ttccaagacg gacggcaatc
     2341 agccgacaac gaactggtcc tgggcgggcg gattgatctc catcggcggt cgccgccaac
     2401 agccgcggga tgtggtcaag gtcgttggtt cgcaaaaacg gggcgacaca caattgagtg
     2461 ttgagaaaaa ccccttccgc ccgggtgatg agatcctcct caccctgcga gacgatgaaa
     2521 agaattcgct gttgacttac ctttaccgcg gccagccggg cgatatctcg gggttgcgaa
     2581 actggtccgt ggagcaggtc tttcggatcg tgagtgtgga aggatcgacg ctcaagctga
     2641 atcgtccctt gcgttttgat atccgctccg agtggcaacc caccgtggag agattcctgc
     2701 cgccgctcgc cgatgtgggc gtggaaggga tcacctttga ttttccggtt cagcagtacg
     2761 cagggcattt caaagagatt ggtttcaacc cagttgaaat cacccgctcc gctgcccatt
     2821 gctggttaaa agaccttgtg atccgcaatg ccgataatgg gccgtttgtc tcgggttact
     2881 tctgcaccgt ggaaaagatt cgctttgaag ccgatccaca gcgggtatca agttcgggaa
     2941 actatacggg tcaccatggc gtgacgtttc tgcgttgctc cgattgcttg tgccgagatt
     3001 tcgaattcaa cacgcgattc atccatgacc tgacggtcca aagttctatt ggttgcgtgt
     3061 tttgttccgg ccgtggtatt gacatctgtt ttgatcatca ccgctgggca ccttatgaaa
     3121 acctcttcac ggacatcgat gcggggacgg gaaaacgcct gttcgcctcc agtggcggcg
     3181 ggatgcgggg taatcacacg gccgggggag aaaccttctg gtgcattcgt acccaggggc
     3241 tggtccgctg gccaaagagc ctgggcatcg atgcgatcaa cgtcgtggga ctgaacgtca
     3301 gcgatgccgc cgaagccgtc ccctcgttgc cgtctccgac agagttaacc ggtcgctggt
     3361 gtgagccaat ctcgccggag gtcctgcaac cgcaaaatct ttacgacgcg atgcgcaaaa
     3421 gggtcgcctc gccgcagtga gcaagagata atgccgcatt tgtgcggtgc cccgtcgagc
     3481 aacgccgggc ccggaaagtt tacaattctc gtcaaataag ttctggactt actccagcgg
     3541 cgcgctagaa ttggcctgta agttgcggcg tactgtggac agcgagatca gatttgcatc
     3601 aaggacgcag tgtgctgctc ccactttgga ctcccggcgt gaatgtccag gcgtattact
     3661 tttgatggca gtacctgtgt tgcctttcct caacacccct tgctaacgcg ttcgctttga
     3721 gaaacatcct tttctgatca cacatggctc ttcagtgtaa gaaccagcct atggtgatac
     3781 ggaggaacgc aaacgaggca tgatcggacc aaccttaaaa ctttggcacg aaacctgcat
     3841 gcaaaagcga cgaggttcca tcgtgcgtgg agagtatccc cgtgttgggc cgagatcata
     3901 acgtgaacaa gaaggtaaac tgatcaggga gctgtcaccc atgactcgtc ttttttcgca
     3961 acgtcgccga cgtccttccc ggcgtggcat cctgagcttc gaatggattc tgcttatctc
     4021 cattcttgtg attgggatta ttggagggct ggccgccgta cgtaacgccc ttctgagtga
     4081 gcttaacgac ctctcacaag ccgtcgaagc gatcaacgtg tgcggcacgt gcgatcccgc
     4141 cagttgcccg ccggggaatc ccgattgctg cgacgatccg tggtggtgtt gcggtccatg
     4201 agcgctgagc tgttccaggc tggcaccaac tggccgcgga tatctctcgg caagcggttg
     4261 acagttgggg atgtcgctac gaaattagga ctcttatggc accccgtgca tccggagaag
     4321 agacgtccct gagtccctcg caccaggcta ctgaaagcaa ctcaccgcgg ggatgtgggc
     4381 ggatgttgcc gcgcctggcg gtggggtgtt tctttcttct catcgccttc ctcttccgtg
     4441 ccttctggct gccggtggtt gcttatcccc tcatggcacc ggcaccgcag cacgagaatg
     4501 ccgttaccct catctgggcc cgtagttcgg atggaaaaac accctccggg gacggtatcg
     4561 tcgagcggac ggcatcactc ctcaagaccc atcctcatgc ccgggtcatt tttacccagg
     4621 ggtggtcaag tcgtttggtc gaactcgggg tgattccgcc accgtacgag acctttcgca
     4681 gcgccctggc gaaatatcag atcgaatccg agcgggttat caacctggag gtgaatggct
     4741 tcgagttctg gaacgaggcc aaggccgtgg atgccttcct ccgcgagcat cctgaggaac
     4801 gggtcctcct actagtggag gaatttgccg ggcaaagcca ccgctatgtc ctccgcaggg
     4861 ccctcgacgt gtcgcatcgc gatcgggtgg agattctagg attgccggat ttgcagtatt
     4921 cgtaccatga ctggtggagg tcccgctccg gcgtgaaggg cgtgatggtg ggttatctgt
     4981 ttctcatcca caccatcctt caaccccaac cgctgcgacc gccccggaag ctctccctgg
     5041 aggactttga acaatcgatc cctgaagcgc caggagggag accatgaaga gggtttggtg
     5101 gcttctcgct tgtctgatcc taagcggcct ggccatcttg ttccacgggc ggtggcttcc
     5161 atccctggcc aagtacctag acgtgggcga ggcaccccgc aaaagcgatt acatcatgat
     5221 tatgggcggc ggtgtcgaga atcggccgtt tacagcggcg gcccttttca aaaaaggttg
     5281 gggagagcgc atcttgatcg ccgaacttcg ccccacaccc ggcggtgaga acctcctccc
     5341 accggagcat gaggtcacaa gtcgcctcct ccagaaattg ggtgtgcccg aggaccggat
     5401 catcgtcttg agaggcaatc acgcggccac gtttcacgag attgtcaccc ttggcgaact
     5461 tctggcccgg gagccggagg cgagggtcat gatcgtcacc gaccccgtcc atactcgccg
     5521 gacccgctgg agcgtgcaac gcattctggg cgaaagggca tcgcggatca gttttgtgtc
     5581 agccccttct gacggatata accccgatga ctggtggcaa actgggcagg ggttttttat
     5641 ggtcacgagt gaatatctca aattgctttt ttatgccttt cgctacgggt ggggactgca
     5701 cctgacggcg gcagcggttg cgctcatcct ccttatcgga atcctcgcgc gctatttgcg
     5761 gccaatgggc cgggataaag cctcgacaag ggaagcggtc cccacctcct gagctccatt
     5821 cgttctggaa caataccgcc ctaccgttga gcggcgaacc ttctcggccg tgccccacga
     5881 ctggttgacc tcacggtaaa atcatgtacc tcggttgaca gatcccaccc tcaatatggt
     5941 ggtgtggtac taccgcatct ggcagccatt tggtaagata ggacgcggtg ttgggggtgg
     6001 gttagtccgt cctccccgca tagacctttt gactgtgttg tgggtggcgg tttcaccaat
     6061 cgagttggtg cactcgagct tgcaaggagt cagggtacca tgactgaatc aagtcacact
     6121 ttctcctcag agaagaaaat ccgtgttttc attgcggatg atcacgacat ggtccggata
     6181 ggcctcaagg ccatgttgtc tgtgccggac atcgaggtgg ttggcgaggc tggtacgggc
     6241 gaggatgcgg tcaatttcct gctcaacaat gatgtcgatg tcgcgatttt ggatgtgcgg
     6301 atgcctcgcg gagacggcct gaatgccctg gggcgaatca agctggaaaa acccgagttg
     6361 ccggtcctca tgctttcaac ctacgataat ccgacctata tcgcccgggc cgtggccttc
     6421 caagcccagg gttatcttct caagagttgt tctcgcgagg aattactcga tgcc
//
LOCUS       DAOPTR010000059         6453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_9876_length_6453_cov_4.241325, whole genome shotgun sequence.
ACCESSION   DAOPTR010000059 DAOPTR010000000
VERSION     DAOPTR010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6453
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9876_length_6453_cov_4.241325"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PKI05_02025"
     CDS             complement(<1..758)
                     /locus_tag="PKI05_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51003.1"
                     /translation="MAMDDKLPNSHQPKPPPLGAKEKPADKPELDPKTSAPVESQPSG
                     AAAQEKTVSGRKVTRSPKAENSQATTTQGISAAAQQREPSSAQAAVQSQEERPRSRVT
                     TLLVEILHWMPSWFVSMVVHAVALLVMAMITIPGPMQDAVQQLVVAPGENEQLEEIEE
                     LLTEPPEDINLATDLSPVEVASTIDSSQDISPFNDEAAAMASFELNPIGLEHAPQTDL
                     LASIGALSGDAFSGRGSDKERMVRTAGGSEGRERA"
     gene            complement(1064..2212)
                     /gene="proB"
                     /locus_tag="PKI05_02030"
     CDS             complement(1064..2212)
                     /gene="proB"
                     /locus_tag="PKI05_02030"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOA51004.1"
                     /translation="MTIDLVRQEIATEARLIVVKVGTRVVTHPDGRLNYERIEQLAEE
                     IHCVRQTGRKVVLVSSGAVGAGVGRLGLPGRPTDLSELQAVAAIGQSLLVEAYDQTFR
                     RYNDVAAQVLLTADVIENRTSYLNARNTLLALLNRFDAVPIINENDTVAVEELRMTFG
                     DNDRLAALVTNLLQAQLLVLLSDVEGLYSGDPNDPKSELIPLVRKIDDSIFDLVRDKK
                     TGLSKGGMASKLKAAQLVTTGGGNVIIASGKTPGVLKKIIAGEVVGTLFFAKGPTVAA
                     RKRWFGLSIQPRGQLIVDAGAREAIEKRGKSLLPIGILDVVGQFQKGDLVALRDPDGC
                     EFGKGLSNYSAEDVRRIKGLRTRQIAQVLGWLPYEEVIHRDNLLVTRP"
     gene            complement(2252..3295)
                     /locus_tag="PKI05_02035"
     CDS             complement(2252..3295)
                     /locus_tag="PKI05_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOA51005.1"
                     /translation="MELQLIRKEQAQPPFFVGVDLGGTNIKAGVTDDRGAVLWVGQEP
                     THVERGPEDGARRIADLIHRAIHEAGLTPQDIAGVGLGSAGTMDIPGGRLVVPPNLKG
                     WEYFPIRDRVSEHTGMPITFANDATAAAFGEKWVGAGHRFHSMILLTLGTGIGCGIIV
                     GDLLLDGEHSHGGEYGHTVIDCRPDARVCSCGRRGHFEAYASATAVTKRAEEALAQGR
                     VSMLTRRIQEGAKLDPKLIAEMAEAGDELSRQLILETAEYLGIGIVNLMHTIDPNGIL
                     IGGAMTFGGKESRIGRIFLERVKHEIFLRAYPAVGDTTEIDFATLGGNAGFIGAAGIA
                     RLEKMKAQFPPAK"
     gene            complement(3459..4559)
                     /locus_tag="PKI05_02040"
     CDS             complement(3459..4559)
                     /locus_tag="PKI05_02040"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOA51006.1"
                     /translation="MLKGNAIVGQSGGPTAVINASLCGVIEGCRESSQIERCLGMRFG
                     IQGFLESDVIDLDRESAETISRLRTTPSSALGSCRYKLRDEDFPRLLEQLKRYNIRYF
                     FLIGGNDTMDTIHRLEEFCRKANYPMLGVGIPKTVDNDLFGTDHTPGYGSAARYVAIS
                     VRQAGMLARDMKRVDQFVIFQTVGRSAGWLAAASALARSAEDDAPHLILLPERPFDRD
                     RFLAAVKQTYDKYGWCSIVCGEGITYPDGTPVSAARVTDQFSNIEFGAMGGTSAAMML
                     HRMISQAFGWRGEFQVCESLQMCAADRVSDIDLEEAYACGREAVRLANEGVSGMMVSI
                     QRESSSPYRYRLGVVPLAEVAIKAKPMPDEFY"
     gene            complement(4760..5182)
                     /gene="nikR"
                     /locus_tag="PKI05_02045"
     CDS             complement(4760..5182)
                     /gene="nikR"
                     /locus_tag="PKI05_02045"
                     /inference="COORDINATES: protein motif:HMM:NF003381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOA51007.1"
                     /translation="MSKIVRFSISLERDLLKKFQRFCREERFATRSEAIRQLIHDFLA
                     RRAWVEDAEDVAGTLTLVYDHHRSQLRDQLMKLQHDHTDLIVSTLHAHLTAHLCLEVI
                     ILRGPAKQLREIAARLRGLKGVHKGELVIAAVPQESPA"
     gene            complement(5228..5980)
                     /locus_tag="PKI05_02050"
     CDS             complement(5228..5980)
                     /locus_tag="PKI05_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007340094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOA51008.1"
                     /translation="MAGHSHWASIKHKKAAVDAKRGKIWSKVSKAIIIAARIGGGDPK
                     MNPRLRVAIEEAKAVGMPKDNIMRAIKRGTGEIEGAALEEVTYEGYGPGGVAIMCDVL
                     TDNRNRTAAEIRKCFELHNGKLGGAGCVAWMFETKGLFLIPADQVNEEQLMDVALEAG
                     AEDIKQVGDKFEVTCDPAKFSDVSEALDRANIPCDSRSVTRIPKDYVDLDVETARKVL
                     KLMEELDEHEDVQNVSSNFNIPDEVMAELSRS"
BASE COUNT         1314 a         1885 c         1787 g         1467 t
ORIGIN      
        1 gcccgctctc tcccttcact cccaccggcc gtgcgaacca tgcgttcctt gtctgatcct
       61 cggccggaaa aggcgtcccc ggacaaagcc ccgatggacg ccaaaagatc ggtctgaggg
      121 gcgtgttcca agccgatggg attaagctcg aacgaggcca tcgcggccgc ctcgtcgttg
      181 aacggggaaa tgtcctgtga gctatcaatg gtgctggcca cctccacagg cgaaaggtcc
      241 gtggccagat tgatatcttc gggcggttcg gtgaggagtt cctcgatctc ttccaactgc
      301 tcattttcac cgggtgccac cacaagttgc tgaacggcat cttgcatcgg tccggggatt
      361 gtgatcatgg ccatgaccag caaggccacc gcatggacga ccatgctgac aaaccagctt
      421 ggcatccagt gaaggatttc cacaagcagc gttgtgacgc gcgagcgggg acgttcctcc
      481 tggctttgaa cagcggcttg agcggatgac ggttcccttt gctgggctgc tgcactgatc
      541 ccctgcgttg ttgtggcctg tgaattctcg gctttgggag accgtgttac cttacggcca
      601 gacacagtct tttcctgcgc cgcagcacca ctgggctggc tctccaccgg ggcagaagtc
      661 ttgggatcga gctccggttt atccgcaggc ttttccttgg ctccgagggg agggggtttc
      721 ggttgatggg aattcggcaa cttgtcgtcc attgccataa cactgtgcct cgaaattctc
      781 cgttccggaa acaatcaccc agtgttttag gacagggaaa tcattcacct tatgacgctg
      841 cccggggtgt tttcttttcg ccgccgaaac accttgggta ggtcagtggt ggtggctcgt
      901 tgatcatggg catagtcaaa cctaccaatg ttattttaac caatgacaca acaacgatac
      961 cagcgttgcc aaccccgcct cttgtcacaa ggcgccggtc ccgctttttc tggcaacgtg
     1021 gtcgtgaaaa cggctcatca tcgtggatca tccgccggcg cgatcaaggc cgggtgacga
     1081 gaagattatc gcggtggatc acttcttcgt acggcagcca gccgaggacc tgggcaattt
     1141 gtcgagtcct gagccccttg atgcggcgga cgtcctccgc ggaatagttg gacaatccct
     1201 tcccgaattc acagccgtct gggtcgcgca gagcgaccag atcacctttt tgaaattgtc
     1261 ccacgacatc gaggattccg atcgggagaa ggcttttgcc gcgtttttca atggcctccc
     1321 gggcaccggc atcgacgatc aactgtccgc gcggttggat gctaaggccg aaccagcgtt
     1381 ttcgggcggc cacagtggga cctttggcga agaacaaggt gccgacaacc tccccagcga
     1441 tgatcttctt caggactccc ggcgtcttac cggaggcaat aatcacgtta cctccacccg
     1501 ttgtgaccag ttgagccgct ttcagtttgc tggccattcc acctttgctc aaaccggtct
     1561 ttttgtcccg caccagatca aagatggaat cgtcaatctt gcgaacgaga gggatcagct
     1621 ccgatttggg atcattcgga tcgccggagt aaagaccttc cacatccgaa agtaacacga
     1681 ggagttgagc ctgaagcaaa ttggtgacaa gtgccgccag gcgatcgtta tcaccaaatg
     1741 tcatgcgaag ttcttcgacg gcgaccgtgt cgttctcatt gatgataggc acggcgtcga
     1801 accggttgag caaggccaga agggtgtttc gcgcgttgag gtagctcgtt cgattttcga
     1861 tgacatcggc cgtcagaagg acttgggcag ccacgtcgtt gtagcggcgg aaggtttggt
     1921 cgtaggcctc gacgaggagg ctttgaccga tggcagcaac agcttggagt tccgacaaat
     1981 ccgtcgggcg ccccggcagc cccaatcggc ccaccccggc acccacggca ccggagctga
     2041 ccaggacgac ctttcgacct gtttggcgga cacagtggat ttcctcggcc aactgttcga
     2101 tgcgttcgta gttcagtcgg ccgtcagggt gagtaacaac gcgggttcca accttgacaa
     2161 cgataagtcg ggcctctgtg gcgatttctt gacgaacaag atcgatggtc ataggtttcg
     2221 ccatgtgttg tgacactgtg ttattgcacc cttacttcgc gggtgggaat tgggctttca
     2281 ttttttcgag gcgggcgatc cccgcggctc cgataaagcc cgcgtttcca ccgagggtgg
     2341 caaagtcgat ttccgttgtg tctcccacag cgggataagc tcgcaggaaa atttcgtgtt
     2401 tgactcgttc caggaagata cggccgatgc ggctctcctt gccgccgaat gtcatcgcgc
     2461 caccaatgag gataccgttg ggatcgatgg tgtgcatcaa attgacaatg ccaatgccta
     2521 ggtattccgc ggtttcgagg atcaattgcc gagagagttc gtctccggcc tcggccattt
     2581 ctgcgattag tttcgggtca agctttgcac cttcctgaat gcgacgtgtt aacatactga
     2641 cccgcccttg ggccagggcc tcctccgctc gcttggtgac ggcagtggcg ctggcgtagg
     2701 cctcaaaatg gccacgtcgt ccgcatgagc aaacccgcgc gtccggccgg cagtcgatca
     2761 ccgtgtggcc gtattcgccc ccgtgactgt gttcaccatc caggagcagg tccccgacga
     2821 tgatgccgca accgatgccc gttcctaagg tcagcaagat catgctatgg aaccgatgac
     2881 ctgcccctac ccatttttca ccgaaagccg ccgccgtggc gtcgttggcg aaggtgatgg
     2941 gcattccggt atgttcacta acgcggtcac gaatgggaaa gtattcccaa cctttgagat
     3001 tgggagggac cacgagtcgg ccgcccggga tgtccatggt tccagcggag cccagaccta
     3061 ccccagcgat atcttggggc gttagtccag cctcgtggat agctcggtgg ataagatcgg
     3121 cgatccgccg tgcgccgtcc tccgggcctc tttcaacgtg cgtaggctcc tgcccgaccc
     3181 aaagtacggc acctcgatca tccgttacgc cggctttgat gttggttcca cccaaatcga
     3241 ctcccacaaa gaaggggggc tgtgcctgtt ccttccgaat gagctgtagt tccattgtca
     3301 gcctcgtaag tctggtatca tcttccgaag attgtgtctc tgcctgcctg gcgccctaat
     3361 tcacaggata acctgcgagc cggacatatt ccggcaaagg tcccaccaat ggccgggcat
     3421 actccacaaa ggcatccgtg acatagagcc tttccttatc aataaaattc atcgggcatg
     3481 ggtttggcct tgatggctac ctcagccagt ggcacgactc ctaaacgata gcggtagggt
     3541 gaggaagact cccgttgaat ggagaccatc atgccgctga caccctcatt tgccagccgg
     3601 acggcttccc gaccgcacgc atacgcctct tcaaggtcaa tgtcgctgac gcgatcggct
     3661 gcgcacatct gaagtgattc gcagacctgg aactctccgc gccagccgaa tgcctgactt
     3721 atcatgcggt gaagcatcat ggcggcactt gttcccccca tggccccaaa ttcgatgttg
     3781 gaaaactggt cggtcacccg ggccgcactg acgggggtgc cgtcgggata tgtgattcct
     3841 tctccgcaga cgatgctaca ccagccgtac ttgtcatagg tctgcttgac ggccgccagg
     3901 aacctgtccc ggtcgaaggg acgttcgggc aagaggatca ggtggggggc atcatcttcg
     3961 gcggaccggg ccagagcaga agccgccgct aaccaccccg cggagcgccc caccgtttgg
     4021 aagatcacga actggtccac gcgcttcatg tcacgggcaa gcattcctgc ctgtcgaaca
     4081 gaaatggcca catagcgggc ggcactgcca taacctggcg tatgatcggt gccgaagagg
     4141 tcattatcca ccgtcttcgg gatgccgaca cccaacatgg ggtaattcgc tttccggcag
     4201 aattcctcca ggcggtgaat cgtgtccatg gtgtcgttac cgccaatcag gaagaagtac
     4261 cggatgttgt accgcttaag ttgttccaaa agtcgtggaa aatcctcgtc ccgcagcttg
     4321 taccggcaac ttcccagagc ggaacttggg gtcgttctca ggcggctgat cgtttccgct
     4381 gattcccgat cgaggtcgat gacgtcgctt tccagaaagc cttggatgcc aaaccgcatt
     4441 cccaggcaac gttcgatctg ggaagactcg cggcaaccct cgatcacgcc gcacaaactg
     4501 gcattgatga cggccgttgg acccccactt tgaccgacga tggcatttcc tttcagcatg
     4561 gtcgcacctc aggattcagt cagttcatcg accggaacgt tgtttgactc cccaccttgg
     4621 cccgcgtttt gcccatacaa gccacggatc aatccacttt gcccattgcc agagcaatcg
     4681 tcaagaccgc atgccgcccg gcggaggaac taaacctggc tccagcgccg tacgcggcca
     4741 aatccgagca ttcaaacgtt caggcaggtg attcctgggg gacggccgcg atgaccaact
     4801 cgcctttgtg aacccccttg agacctcgca accgtgcagc aatctctcgc aattgtttgg
     4861 caggtcctcg cagaataatg acctcgagac acaggtgggc cgtcaagtga gcgtgaagag
     4921 ttgacacgat caggtcggta tgatcgtgct gaagtttcat caattggtcg cggagttgcg
     4981 accggtgatg gtcatagacc aacgtcaacg tccccgcgac gtcttccgca tcttcaaccc
     5041 aggcccttcg cgcaaggaag tcgtggatca actggcggat ggcctcgctc cgagtcgcga
     5101 accgctcttc ccggcaaaaa cgctgaaact tctttaacag atcacgctcc agcgaaatcg
     5161 agaaacgaac gatctttgac acgggatcct cctttagatc ggtgccaccc ttggtgcgtt
     5221 gcctcgacta cgagcgcgat agctccgcca taacctcgtc gggaatattg aaattcgacg
     5281 atacattctg cacatcctcg tgttcgtcga gttcttccat gagtttcaaa actttgcggg
     5341 ctgtctcgac gtccaaatcc acatagtcct tggggatacg ggtcacggag cgtgaatcac
     5401 acggaatatt ggcccggtcg agcgcttcac tgacatcgga gaacttcgct ggatcgcacg
     5461 tgacttcaaa cttgtcaccg acctgtttga tgtcttcggc acccgcttcc aaggcaacat
     5521 ccatgagttg ttcctcattc acttgatctg cggggatgag gaacaaacct ttggtttcaa
     5581 acatccacgc cacacagcca gctccaccca acttaccgtt gtggagttca aagcacttgc
     5641 ggatttccgc cgccgtgcgg ttacgattgt cggtgagcac gtcgcacatg atggcgacgc
     5701 cgcccggccc gtagccctcg taagtgactt cttcaagcgc cgctccctca atttcgcccg
     5761 tgccccgctt aatggcgcgc atgatgttat ctttgggcat cccgacagcc ttggcttcct
     5821 cgatggcgac acgaaggcgg ggattcatct tgggatcacc accgccaatg cgggcggcta
     5881 tgatgatcgc cttggagacc ttgctccaga tcttgccgcg tttggcatca acggcggcct
     5941 tcttgtgttt gatacttgcc cagtgtgaat gaccagccat aaattgctca acccttgttg
     6001 ttcgcagtac ttttccgcag gaggcagcct cctttgctac ggcgacggtt ggatagcgga
     6061 aggaagctcc gagacgtcgt tcacgctcac tatatcacta tatcactggg ccacagcttt
     6121 gaacaagaac gaggggacat tggggcgtgc ctctaggagt ggcaggacaa aaatcatctg
     6181 ccgtgaccgt gtcgtaagaa ttcaccgtcc ggtttccccg gagaaaaggc gggattgttg
     6241 tttttttcct tatcaattcg taaactaaaa ccagtgtggt ccaaggcgga atcggatcag
     6301 cgaacgcctc cgggaggccg atcgtgtggg tttggccggg tgaacgctgc cttccgtctt
     6361 caggtagcgg atgggtgttt gggcattaag tgctgctcag actgtcccca tccacgctgc
     6421 ctgcgggacc gcttcctcat aggtgcggct gtg
//
LOCUS       DAOPTR010000060         6416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_9949_length_6416_cov_3.749410, whole genome shotgun sequence.
ACCESSION   DAOPTR010000060 DAOPTR010000000
VERSION     DAOPTR010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9949_length_6416_cov_3.749410"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PKI05_02055"
     CDS             <1..663
                     /locus_tag="PKI05_02055"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOA51009.1"
                     /translation="GRYSGKSIRVTGSKFFIGRGEDCNLRPASDLVSRHHCVLILDGD
                     YLAVRDLGSKNGTFVNGQRVRTETPLKDGDHLLVGVLEFIVKTGAEAPKAKPKVETVQ
                     EAVARTASASSTEFDVDQWLSEDEVTAESPADADTRTIQVKPAENAGGGPPAPHATPE
                     KPVDEETRKALASLFSGEEGKEEPAPGGKKQGDSGKFKQPQAADTRQAAASVLKSLFR
                     RT"
     gene            682..969
                     /locus_tag="PKI05_02060"
     CDS             682..969
                     /locus_tag="PKI05_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51010.1"
                     /translation="MSNSEPTKDKPQSRKIAGLVGIGLDGDDGQTRVTRGKNFVLWGG
                     SQETHAVMQETAIKVNERLDQKGKRMEDVSVNELRDIIHDVADSMGLKRRD"
     gene            complement(1081..2652)
                     /locus_tag="PKI05_02065"
     CDS             complement(1081..2652)
                     /locus_tag="PKI05_02065"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOA51011.1"
                     /translation="MAQSPLRDFSGREIGEFKILRRLGVGAMADVYLAEQPSLRRQVA
                     LKVLRLDLAQDETYLERFRREAQAAAKLIHANIVQIYEVGSENNIHYIAMEYVPGLNL
                     RQWLQKNGPADLRTALIIMRQVVAALVKAAEEGIVHRDIKPENILVTHNLEVKVADFG
                     LARLRPAEDGLELTRVGMTMGTPLYMSPEQIQGKPLDIRSDIYSLGVTCYQLLTGEPP
                     FKGENAMVVGVMHLTKPPEPLESKRPDLPPSFCRLIHRMLAKDPADRFSSPKVLLAEL
                     RKIYAEHVGEAWPENLPDLESSELSATMTAPAIKKLEEAMRLSQRRTTRRWILAVVAL
                     WVVGLAVGTLIGYWRINEPPLLAGTPTQPTLTVPKYDSIFAQWLHASQLGTEEAWRAI
                     IEYFPEKTYWTSRAKMRLAQIYLREDRLDEALRMFQELAASDDSEPELRAFGLAGMTG
                     VYVLQKRDQEASRALNELWPIRQYLRDPLMQRLVDYSIKSIRTRLGPMTAGDWDTWLN
                     EIKEEASINGPQDRP"
     gene            complement(2716..3288)
                     /locus_tag="PKI05_02070"
     CDS             complement(2716..3288)
                     /locus_tag="PKI05_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51012.1"
                     /translation="MSDDVTFDPYRKWLGIPPEEQPPNYYRLLGIAPFESDPDVIVNA
                     ADRQMGHVRRFQAGRYAQVCQKILNELAAARVCLLDPVRKRAYDQKLFFEFRAAGREL
                     PFPVATPLAQREIPPPWGSEGVPATSGGSFSAVGEKGGAIAGPSVLSLYRRRRKSIWR
                     DPTTAILLTVLFLMVVVVAILVIFRQHFLG"
     gene            complement(3337..3978)
                     /locus_tag="PKI05_02075"
     CDS             complement(3337..3978)
                     /locus_tag="PKI05_02075"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOA51013.1"
                     /translation="MWRHLGFIVLAAAISCCLVSSVFVPTVAAEEEGFQPLFDSKTLN
                     GWEGDPRFWSVKDGVIVGSTDENKTTHNTFLCTTKTYKNFILKLEFRLRNHNSGVQIR
                     SKRLDDFIVAGYQADIADKRYMGILYEERGRGILADVNPEEVAKHVKPNDWNEYVITC
                     DGPKIRIQLNGFTTVDYEEKSPEGAKEGIIALQLHVGPPMQIEFRNIRIKELP"
     gene            complement(4135..>6416)
                     /locus_tag="PKI05_02080"
     CDS             complement(4135..>6416)
                     /locus_tag="PKI05_02080"
                     /inference="COORDINATES: protein motif:HMM:NF022100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HOA51014.1"
                     /translation="TRGIVNDRVWVSYVVLDAHDKPLATFRSQPLGETPSWTLLQLGP
                     FEVDVPAASKAVVELHVEADVNRDVKAEVWFGNVHVHRMPSLRVVLDQPLGLYRLPTR
                     PVVNCHLSGVRSADTPLRITILNAEGKTVHDVELHPKYEKVETAVASAESAIHSQQVF
                     SSQVQWQPDPLTPGFYWLVARIPWKDDAFRETRIPFVLMEAVPGSTSAFFGWSLPQGL
                     EPLGARRLAELITESGVGWIKFPVWYDEQTSPAVLQDVPVLLDRLSNHGVKIIGVLIP
                     PQSVREALGPIAPSHTAGVFLRDQKVWEPSLTLSFVKMASLVPFWQIGGDNEHISAND
                     PSLQKSFVQLKNALGQVVADIRLGVPWRWLEPPPKANIGFISLSEEPPLTAAELENYL
                     ARQSIASEKVFLLVELKPLSRQDYSIGLRVQDLVLRMASVRRASNVIGFHPNPFDPDC
                     GLFYPNGAPAELYLPWRTTIHAIGEGECLGSVALPAGSSNLIFARDDEGVMIVWNNRT
                     VRENVYLGEDIRVTDVWGTTLPYQGGPQGQSFLVDEWPRFVHGVSIPIARWRQEANIA
                     VTRLPSIYGVPHRNKIRWQNTFDRAVSGEVTIVTKPGWRVTPQRFPFHLTPGEKVERD
                     FVITLPFDAEAGPQPVRLDFKVGTDQFTEFSSYHFIEVGMGDVYVEITTRLDESGDLI
                     VQQVLVNDTPETVSFRCELFVPDRRRQFCLIQGQAPGRHVQTYRFPRGEELIGKTLWL
                     RAEEIGGSRILNYRVIATR"
BASE COUNT         1491 a         1828 c         1751 g         1346 t
ORIGIN      
        1 ggccgctatt cgggcaagtc aattcgggtg acgggttcga agtttttcat tggccgcggc
       61 gaggactgta accttcgtcc agcaagcgat ctggtcagtc gccaccattg tgtcttgatt
      121 ttggacggtg attatctcgc ggtgcgggac ctggggtcaa aaaacggaac ttttgttaac
      181 ggccagcggg tcaggaccga aacgcccctc aaggatggcg atcatctctt ggtcggtgtc
      241 cttgagttca ttgtgaaaac gggcgccgag gcccccaagg ccaagccgaa ggtggagact
      301 gtccaagaag cggttgcccg gacagcctcg gccagctcga ccgaattcga tgtcgatcaa
      361 tggttgagcg aggacgaagt aaccgccgaa tcacccgccg acgctgacac gcggacgatt
      421 caggtcaagc ccgcggagaa tgcaggtggt gggccgccag ctccccatgc cacgccggaa
      481 aaacccgtgg acgaagaaac tcgcaaagct cttgccagcc tcttcagcgg tgaagagggt
      541 aaggaggagc cggcgcccgg tgggaagaaa caaggagact cgggcaagtt caagcaaccc
      601 caggcggctg atacacggca agcggcggcc agcgttttaa agtccctctt tagacgcacg
      661 taaatcacgc ggaggaagca cgtgagcaat tcggaaccga ccaaggataa accgcaatcg
      721 cgaaaaattg cgggcctcgt ggggatcggc cttgatgggg atgatggcca gacccgcgtg
      781 acacgaggaa aaaattttgt cctttggggt ggcagtcaag agacacatgc tgtgatgcag
      841 gagacagcca ttaaggtcaa cgaacgcctc gatcaaaagg gtaagagaat ggaggatgtg
      901 tccgtaaacg agttgcgaga cattatccac gatgtagccg attccatggg actcaaacgc
      961 cgggattgag ctgccctcaa ctcatacccg cagtggcatt acgcctactt gcggcttgct
     1021 ttgtttcttg gggtataggc gacaaccagg aacaaggggc aaggtttctc aactggccac
     1081 tcacggccgg tcttgtggac cgttgatgct ggcctcttcc ttgatctcat tcagccaggt
     1141 gtcccagtcg ccggcggtca taggaccgag tcttgtgcgg atggatttga tggaataatc
     1201 gaccaagcgc tgcattaagg gatcgcggag atactggcgg atcggccaaa gctcgttgag
     1261 tgcccgtgat gcttcctgat ctctcttttg cagcacataa acgccggtca ttccggccag
     1321 gccaaacgct cgcagttctg gctcggaatc atcgctcgcg gcaagttcct ggaacatgcg
     1381 gagggcctca tccagccggt cttcccggag gtatatttga gccaatcgca tcttggcccg
     1441 cgatgtccag taggtttttt ccggaaagta ctcaataatg gcacgccatg cctcttccgt
     1501 gcccaactga ctagcatgca accattgggc aaagatgcta tcgtattttg ggacagtgag
     1561 agttggctga gtaggtgtgc cggcgagcag gggtggttcg ttgatccgcc agtaaccaat
     1621 gagcgtgccg actgccagcc ccaccaccca aagcgcgacc acggcaagta tccaacggcg
     1681 ggtcgttcgg cgctgggaaa gacgcatcgc ctcctcgagt ttcttgatgg ccggggccgt
     1741 catcgtggcg gacaattcgc tcgactcgag gtccggtagg ttttctggcc aggcttcgcc
     1801 gacatgctcg gcatagattt tcctcaactc cgctaaaagg accttggggg aggaaaaacg
     1861 atccgcggga tctttggcga gcatccggtg gatcaggcgg cagaaacttg gcggcaggtc
     1921 cggacgtttg ctttctaatg gttcgggggg cttggtcaag tgcatcacgc cgaccaccat
     1981 ggcgttttcc cctttgaagg gtggttcacc ggtgagaagc tgatagcacg tcacccccaa
     2041 cgagtaaatg tcgctgcgga tatccagggg ctttccctgg atttgttctg ggctcatata
     2101 aagtggcgtt cccatcgtca ttccgacgcg ggtcaattcc aatccatctt cagccggtcg
     2161 caaccgagcc aggccgaaat ccgccacttt gacctctaaa ttatgcgtga ccaggatgtt
     2221 ttccggcttg atgtcacgat gaacgatgcc ttcttcggcg gctttcacca gggcggccac
     2281 gacctgacgc ataatgatga gggcggttct caaatcggcg ggaccgtttt tctgcagcca
     2341 ctggcggagg ttcagccccg gcacatactc catggcaatg taatggatgt tgttctccga
     2401 gccaacttcg tagatctgaa cgatgttggc gtggatcaat ttggcagcag cctgggcttc
     2461 acgccggaat cgctccaaat acgtttcgtc ctgggccaag tcaagacgga ggaccttcaa
     2521 cgccacctgg cggcgaaggg agggctgctc ggccagatag acgtccgcca tggccccaac
     2581 acccaaacgg cgaaggattt taaactcgcc gatttcgcgt ccagaaaagt ctcgcagagg
     2641 tgattgagcc atgctgtcat ctcctaccag gggatggttc gccacaatgg ttcgggacgc
     2701 tcacaccacg gtaagctatc ccagaaaatg ttgccggaaa ataacaagga ttgccacgac
     2761 cacaaccatc aaaaacagca cggtcaaaag aattgccgtc gtcgggtccc gccaaatact
     2821 cttgcggcgt cggcgataga gcgacaggac acttgggcca gcgatcgctc cccccttttc
     2881 tcctaccgcg gaaaaagacc cgccactagt tgcaggcacc ccctctgacc cccatggcgg
     2941 ggggatttct cgctgcgcaa gcggtgtggc cacagggaag ggcaactccc gtcccgccgc
     3001 ccgaaactcg aaaaagagct tctggtcata tgcccgcttc cggaccggat caagaaggca
     3061 aacccttgcg gcggccaact cattgaggat cttttggcat acctgcgcat accgacctgc
     3121 ctggaaccgc cgaacgtgtc ccatctggcg atcagcggca ttgacgatga catccgggtc
     3181 tgattcaaac ggggcgattc ctagcagccg atagtaattc ggcggttgct cctcaggcgg
     3241 gatccctagc cattttcggt acggatcgaa tgtgacatcg tcactcattg aatcaaatgc
     3301 ggctcgtatg acgtgatcga accggaaacc ccagctttac ggtagctctt tgattcggat
     3361 gtttcgaaac tcaatctgca tcggcggacc aacgtggagc tgcaatgcaa tgatgccttc
     3421 tttcgcccct tctgggcttt tctcttcgta atcgaccgtc gtaaagccgt tgagttggat
     3481 gcgaatcttt ggcccatcgc acgtgatcac gtactcattc cagtcgttgg gcttaacgtg
     3541 cttcgccact tcttctggat tcacatcggc gaggattccc cggcctcttt cctcatagag
     3601 gatccccatg tagcgtttgt ccgcaatgtc ggcctgataa ccagccacga tgaaatcgtc
     3661 caaccgtttg ctgcgaatct ggacgcccga attgtggttt cgcaagcgaa attccagttt
     3721 cagaatgaag tttttgtaag tcttcgttgt gcagaggaat gtgttgtggg tggttttgtt
     3781 ctcatcggtg cttcccacaa tcacgccgtc ttttaccgac caaaatcgcg ggtctccttc
     3841 ccagccgtta agtgtcttgc tatcaaagag cggttgaaaa ccttcctctt ctgccgccac
     3901 ggttggcaca aacacagatg ataccagaca acacgagatt gccgccgcca aaacaataaa
     3961 gccaaggtgt cgccacatcg tttgtgctcc tctggaactg gacctcagga cagcgaacgc
     4021 gcacaatctg cacgtgctta aagagaccgt aacctacagc cagaatacca ccccattgga
     4081 gcagactcaa gtctgtctct atcccgggca ggcatagcta cgtccgacga gcgattagcg
     4141 ggtcgcaatc acacgataat taaggatacg actccctccg atttcttctg cgcgaagcca
     4201 gagggtcttt ccaatcagtt cttctccgcg tggaaagcgg tacgtctgta catggcgacc
     4261 cggcgcttgg ccttgaatca aacagaactg gcgacgtcga tcgggaacaa acaactcaca
     4321 gcggaaggac accgtctccg gcgtgtcgtt gacaagcacc tgttggacaa taaggtcacc
     4381 ggattcatcc aaccgcgtgg tgatctccac gtaaacatcg cccataccaa cttcaatgaa
     4441 gtgatagcta ctgaactcgg taaattggtc cgttcctacc ttgaaatcca accgcacggg
     4501 ttgagggccc gcctcggcat caaacggaag ggtaatcaca aagtcgcgct caactttctc
     4561 acccggcgtc aagtgaaaag gaaatcgctg aggtgtcacc cgccaccccg gtttggtcac
     4621 gatcgtcacc tcacccgaaa ccgcccgatc aaatgtattc tgccagcgaa tcttattgcg
     4681 atgaggcaca ccatagatgc tgggcaaacg ggtgaccgcg atatttgcct cttgcctcca
     4741 acgagctatg gggatggaaa cgccgtgaac aaaccgaggc cactcgtcaa ccaaaaatga
     4801 ctgcccctgc ggaccacctt gataaggcaa tgtcgtcccc cagacatctg tcacgcgaat
     4861 atcctcaccc aggtacacat tctctcggac cgttcgattg ttccagacaa tcatgacacc
     4921 ttcatcgtct cgggcaaaaa tcaggttgga agatccagcg ggtagggcta ccgaccccaa
     4981 gcactcgcct tccccaatgg cgtggatggt cgtccgccaa gggagataca actccgcggg
     5041 cgcaccattg ggatagaaca atccgcagtc ggggtcaaaa ggattagggt gaaagccaat
     5101 gacgttgctg gcccgcctca cactggccat ccgcaaaacc agatcttgaa cgcgaagtcc
     5161 aatcgagtag tcttggcgac tcagtggctt gagctcgaca agaaggaaga ccttctccga
     5221 agcgatcgac tgccgagcca aatagttctc caactcagcg gccgtcaacg gtggttcttc
     5281 agacaaagag atgaagccga tgttggcctt cgggggtggc tccaaccacc gccaaggtac
     5341 gcccaaacgg atatcagcga cgacctgccc taaagcgttc ttcagttgga caaagctctt
     5401 ttgcagcgat ggatcattgg ccgaaatatg ctcgttgtcc cccccgattt gccaaaacgg
     5461 aaccaaagag gccatcttca cgaagctcag cgtcagcgaa ggttcccaca ccttctgatc
     5521 gcggaggaat accccggccg tgtggctagg agctatggga cctagtgcct cacgaacgct
     5581 ttggggagga ataagcacac caatgatctt gaccccatga ttgcttagcc gatccagtag
     5641 cacggggacg tcttgaagga cggcgggaga ggtttgctcg tcataccaca caggaaactt
     5701 gatccaacct acgccagatt ctgtgatcaa ctccgcaagc cgccttgcac ccagtggctc
     5761 taatccttgc ggcagtgacc acccaaagaa cgcacttgta ctccccggga cagcctccat
     5821 gagcacaaat gggattcggg tttcacgaaa ggcatcgtct ttccagggaa tgcgggccac
     5881 cagccagtag aaaccgggcg ttaatgggtc cggttgccac tggacctgag acgagaacac
     5941 ctgttgactg tgaatcgcgg attcagccga tgccaccgcc gtctccacct tttcatactt
     6001 gggatggagc tcaacatcgt ggacagtctt tccctccgca ttaagaatcg tgatgcgaag
     6061 cggtgtatcg gccgaccgaa caccggagag gtgacagttc accaccgggc gggtaggcag
     6121 tcgataaagg cccaacggct gatcgaggac gacgcggagc gacggcatcc gatggacgtg
     6181 aacgttgccg aaccacacct cggccttcac atcacgattg acgtcggcct ccacgtgtag
     6241 ttcgacaacc gctttggaag ccgccgggac gtccacctca aaaggcccca attgaaggag
     6301 tgtccaactt ggcgtttcac ccaagggttg tgaccggaac gtggcaaggg gcttatcatg
     6361 ggcatccaag acgacataac tcacccaaac acggtcgttc acgatccccc gggtct
//
LOCUS       DAOPTR010000061         6391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_9996_length_6391_cov_3.666667, whole genome shotgun sequence.
ACCESSION   DAOPTR010000061 DAOPTR010000000
VERSION     DAOPTR010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9996_length_6391_cov_3.666667"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1381
                     /locus_tag="PKI05_02085"
     CDS             <1..1381
                     /locus_tag="PKI05_02085"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HOA51015.1"
                     /translation="NGWPLAFQKRFANNFAAQAWLWKDHTSYAEAAEITEKARAMGLP
                     YLAKCGGTYSSEWFWSKILHCLRTNPNVFDAAYSWVELADFIPGFLTGNLKPETIPRS
                     ICAAGHKAMYNPEWGGLPSEEFLRALDPKLVKIRQRYAPEALPADRKAGELTREVARK
                     VGLRPGTPVAVGAFDAHMGAVGAGIKPGTLVKIIGTSTCDMMVVPMNQPLPDIPGLCG
                     IVPHSIIPGMYGLEAGQSAVGDIFNWFVSFLAPAKYTAAGDPHAALTRDAEKLKPGES
                     GLLALDWNNGNRTILVDPLLTGLLVGQTLLTTAPEVYRALIEATAFGALTIIKRFEEY
                     GVTVKEVVNCGGIAEKNPFVMQIYADVCNRPMKISRSAQTCALGAAIFGAVAGGAYKD
                     VEQAQRRMTGTKPTVFRPRKQAVAVYERLYQLYRRLHDAFGLPDYRDSLYDVMKQLIA
                     LRKEVRGES"
     gene            1494..2195
                     /locus_tag="PKI05_02090"
     CDS             1494..2195
                     /locus_tag="PKI05_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOA51016.1"
                     /translation="MSLEQLREEVCRANKLLMEHGLVCLHSGNVSGLDRSTGRLVIKP
                     SGVDYETLSPEDLVEVDLITGKVISTHLRPSVDLPHHLYIYRNMPGVGGIAHAHSDYA
                     TAWAAVGRPVPLCLTSMADYFGGEIPCAPYVDNEGDNIGQAIVRYRGRAPAILLANHG
                     AFTWGPTALDAVKLAVALEDAAKKAFLASQLGQPLEIPLEEARKWYDKYHHGYGQIVP
                     PRTPQTVTRQPQSPG"
     gene            complement(2261..3250)
                     /locus_tag="PKI05_02095"
     CDS             complement(2261..3250)
                     /locus_tag="PKI05_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51017.1"
                     /translation="MEKHVSRRQFVGTTLFGSTVGALSCQALTRMTVAEEPASAPVTS
                     LPKGKIKDLEISRLILGGNLLTHFTHSRDLRYVYTLARHYNTDEKILETLAVAEAHGI
                     NTLFIHYVPHALSILQEHRKRGGKMQWITCTAHALVSGGLDEFKRQIDALVAAGTNAL
                     YISGLEGDFSCGFLNDVCGPEADERVGAPRMELLAQALDYAKSHKLPVGIGAHRMGVV
                     EDCEKAKLDCDFYVVTFHRQNYPSAKLYYDSRWCSKPEELAALMQSVEKPWFAFKVMA
                     AGAIPPEVAFRYAFENGADFVLAGMFDFEIATDVALVNKILAELKNRQRPWRA"
     gene            complement(3383..4225)
                     /locus_tag="PKI05_02100"
     CDS             complement(3383..4225)
                     /locus_tag="PKI05_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOA51018.1"
                     /translation="MIELGFHTDNWRPLSKSFEYAVEMGAALGLKHVEFAVINGQDFI
                     QEMGYDPAISLQSNPRALRRYLESKGLSVSQIDGAYPLMGPKGSAFGVHYVQQTIRFA
                     AELNCPIVDTTDGAVEVPGLSRDEVFRITCDNYRQCLSWAEDYGIILAVEPHGPYTND
                     PEFLHRLFDYFQSEYLRFNLDTGNTFIAGHDPLEFCKEFRPYLVHCHIKDVSESLAAA
                     ARGQDTGIGTSFVPVGGGVNAENIRRVIQYLKETNWSGAVSIECYGTQEFMEASVKFL
                     RSLL"
     gene            complement(4646..>6391)
                     /locus_tag="PKI05_02105"
     CDS             complement(4646..>6391)
                     /locus_tag="PKI05_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51019.1"
                     /translation="VDSSGSATNAEGKAYGTDPLLPNEDAIKPFKYPASDPRLQNSEG
                     YPDWFLLRRGQTHDYFEIALTGGRSKKEPMVVAAYGPLADGRAVVAPKGRSPFGAHTG
                     GRTVVQFHQVLSGLEIYGGLSHLGMHAGCTSPGEPGVPTWLIEDCKLINCQMNYLPID
                     TTVRKCVSMFRWSAKSHNQGYFTSGFDAAPTFEEVIFYKNGYKDDPRIDPDPRRTIFD
                     RNIYQGGGAQMGHTYRNIISADGGSGGPQMRLGGLCENSLIIEGYWFSSTASNRLFNP
                     WLTAGKQTGCSAIVRNNVQFVYVYPSPADPDTDGKSDRRAQPGHGYTMQGASFGGVVE
                     NNIVSGAMLTDDPGFSRPVGQAAFSLSPGRGQYEDGNYYTMQRCTIRGNIGYRTKFGL
                     RLTGDWTDTMNLVVENNVFVAEGIVSSDAEHLTSANQLVVRNNRFYVANDDLPQAVWM
                     GTDNTVAPRDEAAAREKWPDPDRTLKRYVQEVLKLTLLDWSDDPWLDRDEVARRVKAG
                     EPYDPTGLKTFMAVATNMRRGGTDPIPTSGKPSWTGDYPWDARFTGQAVVNWVREGFG
                     MKPVEASGESPEKTR"
BASE COUNT         1374 a         1843 c         1757 g         1417 t
ORIGIN      
        1 aaatggctgg ccgttggcct ttcaaaaacg ctttgcgaat aactttgccg cccaggcctg
       61 gttgtggaag gatcatacgt cctatgccga ggccgcagag atcaccgaga aggctcgggc
      121 aatggggctt ccgtatctcg cgaaatgcgg cgggacttac agctcggagt ggttctggtc
      181 gaagatattg cattgtctcc gaaccaatcc aaacgtcttt gatgcggcct actcctgggt
      241 ggaattggcg gactttatcc ccggcttttt gacagggaat cttaagccgg agaccattcc
      301 acgctcgatc tgtgccgccg gacacaaggc gatgtacaac ccagaatggg gaggcctgcc
      361 cagtgaggaa ttcttgcgtg ccctcgatcc caagctggtg aaaattcggc agcgatatgc
      421 gccggaggca ctaccggccg atcgtaaggc aggtgagcta acgcgggagg tcgcccgcaa
      481 ggttggtttg cggcccggaa cgccggtggc cgtcggggcc tttgatgcgc acatgggagc
      541 ggttggcgcc gggatcaaac cgggaaccct cgtcaagatt atcggtacta gtacctgcga
      601 catgatggtt gtgccgatga atcagccact cccggatatt cctgggctgt gcggaattgt
      661 gccgcactcg attattccag ggatgtacgg gctggaggcc gggcaatcgg ctgtgggaga
      721 catcttcaac tggttcgtaa gttttctggc accggctaag tacaccgccg cgggcgatcc
      781 ccacgcagca ttgactcgtg atgccgagaa attgaaaccc ggcgagagcg ggctcctggc
      841 cttggactgg aacaatggca accggacaat cctcgtcgat cctctgctca cgggattgct
      901 cgtcggtcaa acgctgttga ccacggcccc ggaagtctat cgtgccctca tcgaggcaac
      961 cgcctttggg gccttaacga tcatcaagag atttgaggaa tatggtgtga ctgtcaaaga
     1021 ggtcgtcaac tgcggaggca tcgccgagaa aaaccccttc gtcatgcaga tttatgcgga
     1081 tgtctgcaat cgaccgatga agatcagccg atcggcgcag acgtgtgcct tgggggcagc
     1141 cattttcggt gctgttgcag gcggggcata taaagatgtt gaacaagccc aacgtcgcat
     1201 gacgggcacc aagccgaccg tctttcgacc acgaaaacaa gcggtggctg tttatgaacg
     1261 cctttatcaa ttgtaccgtc ggcttcatga cgcctttggc ttgcccgatt accgcgattc
     1321 cctgtacgat gtcatgaaac agctcattgc cttgcgaaaa gaagtccgcg gcgaatcgtg
     1381 aaccaggtgt aggcgtcgca tggtaagccg caggttgtct gcgtaacaca gttgccgtgt
     1441 tgcgccggca gcagtgggca gcttttcccc ctgatcagcg ggagtcacct gagatgagtt
     1501 tagaacagtt gcgagaagag gtttgtcgtg ccaacaagct tctgatggag cacggtctgg
     1561 tctgcctgca ttcgggaaat gttagcggac tggatcgcag caccggccga ctggtcatca
     1621 agccttccgg cgttgactat gagacactct ctccggaaga cctcgtcgag gtggatttga
     1681 ttaccggcaa ggtcatcagt acccatctgc ggccgagcgt ggatttgccg caccatttat
     1741 atatttaccg gaatatgccg ggggtcggag gtattgctca tgcccatagt gactacgcca
     1801 cggcttgggc ggccgtggga cggccggtcc ctctttgctt gacatccatg gccgactatt
     1861 tcggaggtga aatcccttgt gctccgtacg tggataatga gggcgacaac atcggccagg
     1921 ccatcgttcg ttatcgggga cgtgcccccg ccatcctctt ggccaatcac ggggctttca
     1981 cttggggacc gaccgcgctg gacgctgtca aactggccgt ggcgctagag gatgcagcca
     2041 agaaagcgtt tttggcctct caactgggcc aacccctgga aatcccattg gaagaggcgc
     2101 gaaaatggta cgacaagtac catcatggat acgggcaaat cgttccccca cggacacccc
     2161 aaacggtcac tcggcaacca cagagccccg gctgaacaca ctctcgaccg gtcaccgcgg
     2221 ccaacgacca cagacattcc gcttattcat gcgtcttaca tcatgcccgc cagggccgct
     2281 gtcggttttt caactcggcg agaatcttgt tcaccaaggc cacatccgtg gcgatctcga
     2341 aatcaaacat ccccgctagt acgaaatccg caccgttttc aaaggcatag cggaaggcca
     2401 cttcaggcgg aatggcgccg gcggccatga ccttgaaggc aaaccagggc ttctccacgg
     2461 actgcataag ggcagccaat tcctccggtt tggagcacca ccgcgaatcg taatagagtt
     2521 tggcgctggg atagttctgc cggtggaatg tgacgacata gaagtcgcaa tcgagtttcg
     2581 ccttctcgca atcctccacg actcccatac ggtgtgcccc aattcccaca gggagcttgt
     2641 gcgatttggc gtagtcgagc gcttgagcca gcagttccat ccgcggtgct cccacacgct
     2701 cgtcggcctc cgggccacaa acgtcgttaa ggaagccaca ggagaagtcg ccctccaacc
     2761 cagaaatgta aagtgcattc gtacccgcag ccacgagggc atcgatttga cgtttgaatt
     2821 catcgagtcc cccgctgacc aaggcgtgag ccgtgcacgt gatccactgc atctttccgc
     2881 cgcgcttgcg gtgctcctgc aggatactga gagcgtgagg gacataatgg ataaacaggg
     2941 tgttaatccc gtgagcctcg gccacggcaa gtgtttccag gattttttcg tccgtgttgt
     3001 aatgtcgggc caaagtatat acgtaacgca aatcgcggct atgggtgaaa tgcgtcaaaa
     3061 gattacctcc caaaataagc cggctgatct ccaggtcttt gattttgcct tttggcagcg
     3121 atgtcaccgg ggcgcttgcg ggttcctccg ccaccgtcat ccttgtcaat gcctggcagg
     3181 ataaagcccc cacggtggag ccaaagagtg tcgttccgac aaactggcga cgcgaaacat
     3241 gcttttccac ggttaaacct ccctattgtt ggcgatggat acggcgagca acacacactt
     3301 tttccggcac ttttccccac agtcgatcag acaactcagt ctcggttatc tgtttccttc
     3361 ttgccgtttt ctaggtctta cctcacagaa gcgatcggag aaacttgacg ctggcctcca
     3421 tgaactcctg tgtcccgtag cattcgatgg acacggcacc actccaattc gtctctttga
     3481 gatattggat cacccgccgg atgttctccg cattaactcc cccgccaacc gggacaaagg
     3541 acgtcccgat tcctgtgtct tgcccacgag cggcagccgc cagtgattcg ctcacgtcct
     3601 tgatgtgaca atgaacgagg tagggtcgga actccttgca aaactccagc ggatcatgac
     3661 cggcaatgaa cgtgttgccg gtgtccagat taaaacgtag gtattctgac tgaaaataat
     3721 cgaacagacg atgcaaaaac tccgggtcat ttgtgtaagg accgtggggt tcaacggcca
     3781 ggatgatccc ataatcctcg gcccaactta ggcactgacg gtaattatcg cacgtaattc
     3841 ggaaaacctc atcgcgactg agtcccggaa cctcgacggc cccgtccgtg gtatccacga
     3901 tcggacagtt tagttccgcc gcaaagcgga ttgtctgctg gacgtagtgc acaccaaagg
     3961 cggagccttt tggccccatc aaaggatagg caccgtcaat ttgagaaacg gatagtccct
     4021 tcgattctag gtaacggcgc aaggcccgcg gattactttg aagagaaatg gccggatcgt
     4081 agcccatctc ctggataaaa tcttgtccgt tgatgacggc aaactccaca tgtttgaggc
     4141 ccagcgcagc ccccatttcc actgcgtact cgaagctctt gctaagcgga cgccagttat
     4201 ccgtgtgaaa tcccagttcg atcatttgaa accctcccgt ttcgccaagg ctgcaacgca
     4261 gtacgacatc gcaacaactc tagcccttcc caccaacctg gaatgttacg ctcacgaatt
     4321 ccaggccctt acattgtcgg aatcggagtc gttgttgaca agcgaaagag cgacacaagt
     4381 cgaatgatcc tccatccaag gtcgcggatg cgatctgaga agatcgacca cgtggcatac
     4441 ggcgggcttc cgggcctcaa agttacggct cctcgtgatc tcgcgcctat ccccggaggg
     4501 cacatgatcg cctctcattg aacctccgcc gtctcgcacc gcttgcctgc cgcattcgct
     4561 ggcagcaccg tcgcttccgc atttagcgat ccacttgcgc cgaggagcta tccagccttc
     4621 cccgattgat ctgatgatcg cagcctcagc gggtcttttc tggtgattcg cccgacgctt
     4681 cgacgggttt catcccgaat ccctcacgga cccaattgac cacagcttga ccggtaaacc
     4741 gagcatccca cggatagtcc cctgtccagg agggcttgcc tgacgtggga atcggatcgg
     4801 tgccgccccg gcgcatgttg gtggccacag ccatgaacgt tttcaagccg gtaggatcat
     4861 agggttcacc ggccttgacc cgtctggcca cctcgtcgcg atcgagccag ggatcgtctg
     4921 accaatccaa cagcgtgagc ttgagcactt cttgcacata tcgtttgagc gtgcggtcag
     4981 ggtccggcca tttctccctt gcagccgctt cgtcgcgggg agcaactgtg ttatctgttc
     5041 ccatccagac cgcctgggga agatcgtcgt tggcaacgta aaagcgattg tttcgcacaa
     5101 ccaactggtt ggcggaggtc aggtgctcgg catcgctact tacgattccc tcggcaacaa
     5161 acacgttgtt ttcgaccacc aaattcatcg tgtcggtcca atcgccggtc aggcgaaggc
     5221 caaattttgt ccggtagcca atattccctc gaatggtaca acgttgcatg gtgtaatagt
     5281 tgccatcttc atactggccg cgccccggcg ataggctgaa agcagcctga ccgacgggcc
     5341 gggagaaacc ggggtcatcc gtgagcatcg cgccgctcac aatattgttt tcgactacac
     5401 caccaaacga tgccccttgc attgtgtaac cgtggccggg ctgcgcccgc cggtccgatt
     5461 ttccatcggt gtcagggtcg gcaggactgg gataaacgta cacaaactgc acgttgttgc
     5521 gaacgattgc cgagcaaccc gtctgcttgc cagcggtcag ccaggggtta aagagacggt
     5581 tgctggcggt agaactgaac cagtagccct cgatgatcaa actgttctca cataaccctc
     5641 ctagtcgcat ctggggacca cctgagccgc catcggccga gatgatgttg cggtaggtat
     5701 gccccatctg cgctccacca ccctgataga tgttgcggtc gaagatggtg cgtctgggat
     5761 cagggtctat tctgggatca tccttatagc cgttcttgta aaagatcacc tcttcaaagg
     5821 taggggcggc atcgaagcca cttgtgaagt agccctgatt gtgggatttg gcagaccagc
     5881 gaaacatact tacgcatttg cgcaccgtcg tgtcgatcgg caggtagttc atttggcagt
     5941 tgatcagctt gcaatcttcg ataagccacg ttggtacgcc cggctccccc ggggaagtgc
     6001 agccagcatg cattcccaag tggctcaatc ccccataaat ttccagtccg ctgagaacct
     6061 ggtgaaattg aacgactgtc cgtccgccgg tgtgtgcccc gaatggcgac cgccccttgg
     6121 gagcaacaac ggctctgcca tctgccagcg gcccgtaggc cgccacgacc atcggttcct
     6181 ttttgctgcg gccaccggtc agtgcgattt cgaaataatc atgcgtctgt ccacggcgta
     6241 gcagaaacca atccgggtat ccctcactgt tttgcagtcg cggatcgctc gcgggatact
     6301 tgaagggctt gatggcatcc tcattgggca ggagagggtc agtgccatag gctttccctt
     6361 cggcattggt cgcgctaccg gaggagtcaa c
//
LOCUS       DAOPTR010000062         6374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_10020_length_6374_cov_3.134357, whole genome shotgun sequence.
ACCESSION   DAOPTR010000062 DAOPTR010000000
VERSION     DAOPTR010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10020_length_6374_cov_3.134357"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PKI05_02110"
     CDS             complement(<1..509)
                     /locus_tag="PKI05_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51020.1"
                     /translation="MSRSFALLVCSVTLWAYTAYSDSVFAQGVKGFFHSIKVDAVRNA
                     CWPDAFVPADRTNARLPFVIMVHNGWKRQNLIADHYFAEDGKTLTDAGKRQVQWIVTQ
                     APLSHRAIYVKEGEDATITEARVKAVGEVASKYLGPEQKPDIRVTNISPPGWPAELVD
                     TVERAYLKT"
     gene            1057..2004
                     /locus_tag="PKI05_02115"
     CDS             1057..2004
                     /locus_tag="PKI05_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOA51021.1"
                     /translation="MVAAGGVTTAGSAQFDPLSSVKDGLEKVGELLTFTEPVQQAPDP
                     TALSTKAKPSPRLYVAMARFAEESRQPAMAEQYYEKALSMDAKYLDALLGLGRLKDRQ
                     GQLEEALTCYQKATTAHPGEPTAWNHLGLCYARMNRLDHAKTAFEKAISLSPKESRYR
                     HNLAAVLVQMGDLQGALEQLQAVHDEATAYYNLGYLLVRKGDHAAASRYFAHAVQVKP
                     DFQEARQWLDALSQRSADASKQSQMPPNVELPTPVERPALPDQNLPVPPSGPAGTSPR
                     AFQSAHQVAPLPPVLDENTLEVSDWPSLSAPRPLPPVVE"
     gene            2487..3218
                     /gene="hisA"
                     /locus_tag="PKI05_02120"
     CDS             2487..3218
                     /gene="hisA"
                     /locus_tag="PKI05_02120"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOA51022.1"
                     /translation="MEVWPAIDIRGGKCVRLCQGDYGREIVFNSDPVAIAHHWVTLGA
                     RRLHIVDLDGAKIGEPVNFDIVQRIFQESGVPCQFGGGVRDETTISRLLDAGASRVVV
                     GTRAVKDPDWFRAVVRKFPRRIVLGLDVRAGRAATDGWLEISNRTAAEIVAEFADEPI
                     AAVVFTDISADGMLSGVSLEAFRHMQQVITVPLIASGGVTTLEDIRALKEIGVTGCII
                     GRALYEGRLNLPEVLAIAEDKGSCQ"
     gene            3332..5434
                     /locus_tag="PKI05_02125"
     CDS             3332..5434
                     /locus_tag="PKI05_02125"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOA51023.1"
                     /translation="MALYEVKKIRNIALCGHGSVGKTTLLDRLLNFTGAVNRPASVDD
                     GTSICDFDEEEKSHRYTIEAKVTFFDYDGLHYHVIDTPGYRDFIGQVIGAFRAVDLAA
                     IVINAQSGVEVNTRRVFQEAEKAGIPRLIIINKLDAENIDFENLLAGIRETFGDRCVP
                     LNVPIGLGADFRGVATTLKAESSVPGAVIDPSSISQSLMESIVVADEEVLSRYFEGQM
                     PTDEEISQLLGRAMINGSVIPIVCVSAKTGVGLPELLKVFDLCGISPDRIVRRAQKPD
                     SGEWVELTADPSGPLVAQVFKTRVDPFVQKISFLRAYSGTVRTNATVSVVGERKGLKL
                     AQLFHVQASEMIPIEEAGPGAIVAVTKVEELKTGMSIGDLVMPPIEFPTPMTGLAITP
                     KSRGDEQRLSGALAKICQEDPTCRLDRDPQTKELVITGMSELHLQVIRERLKRRDKVE
                     VDVKEPKIPYRETIQAPAEGMYRHKKQTGGRGQFGEVHIRMFPFPRGTKVEEFATKER
                     FPEMKEVHYHEESNFLWIDSIVGGTIPSNFMPAVEKGFLERISRGVIAGYPVQDVCVE
                     VFFGKHHPVDSSEQAFKTAASMVFRNVFQQAKPALLEPIVKIEVTVPADKVGDINSDL
                     SGRRGRVLGMTSAGGGFQTIVAEVPLAEVITYARALSSITGGQGSYTLEFSHYDIVPG
                     NIQKEIIEKAKLEEEEEE"
     gene            complement(5596..5907)
                     /locus_tag="PKI05_02130"
     CDS             complement(5596..5907)
                     /locus_tag="PKI05_02130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HOA51024.1"
                     /translation="MIHVIAEIVTKPGQRDAVLAEFQKIAPAVRQEPGCLEYGAALET
                     AAGLPVQPPVRDNVITVIEKWESLELLHQHLGAAPLQSFLQKTQHMIESLSARVLSPV
                     G"
     gene            complement(5951..>6374)
                     /locus_tag="PKI05_02135"
     CDS             complement(5951..>6374)
                     /locus_tag="PKI05_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOA51025.1"
                     /translation="TPVCLDESITTPAQADMALELHSAKYLNVNPIRVGGLTPAKAIH
                     DAAHQHCVPCYLGLYPGTTISVRAALALASLSNFCYPPDYWDTAEAFVEDVAEPLQTV
                     RGDQNGRLTVSFWSDAGLGVDPTAEVLGKYCLSECHLP"
BASE COUNT         1446 a         1731 c         1802 g         1395 t
ORIGIN      
        1 atcgttttga gataagccct ttccacagtg tcaacgagct cagccggcca accgggtgga
       61 ctgatgttgg tcacgcgaat gtcgggcttt tgctcaggtc caaggtactt gctcgccacc
      121 tcgcccacgg ccttcacacg ggcctctgta atggtggcgt cttcgccttc cttaacataa
      181 attgcccggt gacttagcgg tgcttgggtc acgatccact gaacctgccg tttcccagca
      241 tcggttaacg tctttccgtc ttccgcgaag tagtggtccg caatcagatt ctgtcgtttc
      301 cagccattgt gaaccattat cacaaacggc agacgggcat tcgtgcggtc tgccggaacg
      361 aacgcatcgg gccaacaggc attgcggacg gcatcgacct tgatgctatg aaagaatccc
      421 ttcacacctt gggcgaaaac ggaatccgaa taggctgtat acgcccataa agttacacta
      481 caaacgagaa gtgcgaagga cctcgacatt tccttgtcct catgcgtgat aactgaatca
      541 ccaaattccg cagtgggaat ggtatcatcc accgttccgt ggctcttctt cgtgtgttat
      601 cggcaccttt cacttgactc gtggatactt attacgaaaa cccccataca caatcgaaag
      661 gtgcacaaag tagagaaaat ggaggccctg catgggcttt catgatgagt ttaccttggt
      721 tagctcctat tgttcttgat ttttctgcgc cgcctgccga tgactcctat gcctgttgcc
      781 ggtagtcagg agcgctacct tgcgcgcgaa actgggcaac atacgagaga tgacgccggg
      841 cccacaaacc acggagggtt gcacatgggc caaaaggatt gcggaaattt gtgcgttgcg
      901 ttcccgatta gccttctatt actaggcatt actatttgcg gctgcgcgtc tgatccttca
      961 aagcagtccc ttgggggaaa atccggtccg acagggctcg gaataatctc accgggaacc
     1021 ggtacagctc agactgggaa cactcgaaag agcggcgtgg ttgccgcggg aggtgttacc
     1081 accgccggtt cggcccaatt tgacccactc agtagcgtta aggatggcct ggaaaaggtc
     1141 ggggaactac tcacgttcac ggagccggtc caacaggctc ccgacccaac agccctatcg
     1201 accaaggcca agccgagtcc ccggctgtac gttgctatgg cacgttttgc ggaagaatct
     1261 cgtcaacctg ccatggccga acagtattat gaaaaagccc tcagtatgga tgccaaatac
     1321 ttggacgcgc ttcttgggtt agggcgtctt aaggacaggc agggtcaatt ggaggaggcc
     1381 ctcacctgct accaaaaagc aacgacagcc catccggggg aaccgacggc ttggaatcac
     1441 ctgggcttgt gctatgcccg gatgaaccgg cttgaccatg ccaagaccgc gttcgaaaag
     1501 gcgatcagcc tctcaccgaa agagtcccgg taccggcaca acctagccgc ggtgctcgtg
     1561 caaatgggcg atttgcaagg ggcgttggag cagcttcaag cggttcatga cgaagccact
     1621 gcctattaca atcttggcta tttgctggtc agaaagggag accatgcggc ggcttcgcgg
     1681 tatttcgccc acgcagtcca agtcaagcca gacttccaag aggctcggca gtggctagac
     1741 gccctttccc aaagatcagc ggacgcgtcg aagcaaagcc agatgccccc caatgtggag
     1801 ctgccaacac cagtcgaaag acctgcattg cccgaccaga acctaccggt acctccctcg
     1861 ggtccggccg gcacttcccc gcgagcattt cagtccgccc accaagttgc ccccctaccg
     1921 cccgttcttg acgagaatac gcttgaagtg tctgattggc cgagcttgtc agcacctcga
     1981 cccctacctc ctgtcgttga gtaacgatgg ggcctgggtc aaggaaggcc tgacgcgcgt
     2041 ccgacgaaac ccatttgccg gttttagagc ctatcccata atctcccctc acccgttcgg
     2101 caggagaggg gtcgggggtg agagtggctc gaatcgctac ccgttttctc aacaagattc
     2161 cgacgttcgc tcgccctcac cccaaccctc tcccggaggg agaggggaag ctttttggga
     2221 aaggcttcta acctcaacat ctcagcgctt tctgcgcata gacaaggagc ttctgcgcat
     2281 agacaaggag cacctccttg tcggtttcca ctccccatgc agccgaccga aatcagtctg
     2341 ttgtcgtcta acagaaggtt ttgaggtccc ggttctcctc tttgtttcta agcttttcgc
     2401 agcgtgtctt gaaggactag ctcctgtgtg tcatattaca cgttttacga tgttggaaaa
     2461 aagcgcgtca gttgaagggc caccccgtgg aagtctggcc tgcaatcgat attcgtggcg
     2521 gaaaatgcgt caggctatgt caaggcgatt acggtcggga aatcgtattt aattccgacc
     2581 ctgtcgcaat tgcgcaccac tgggtcacgt taggtgcgcg gcgcctacat attgtggacc
     2641 ttgatggggc gaaaataggg gagcctgtca acttcgacat cgtgcagagg atattccagg
     2701 agagtggggt tccctgccag tttgggggag gggtccggga cgaaaccaca atttcgcgcc
     2761 tcctggatgc tggagcatcg cgcgtggtcg ttggaactcg ggcggtgaag gacccggatt
     2821 ggttcagggc ggtcgtccgg aagtttcccc gtcggatcgt cctcggactt gacgtccgag
     2881 ccggtcgcgc ggcaaccgat gggtggttgg agatctccaa ccgaaccgcc gcagagattg
     2941 tcgcggagtt tgcggacgag cccatcgcgg cggtcgtttt cacagacatt agtgccgacg
     3001 gcatgctctc cggtgtgagt cttgaggcct tccggcacat gcagcaggtc atcacggtgc
     3061 cgttaatcgc atccggaggc gtgacaacac ttgaggacat cagggcactt aaggaaattg
     3121 gggtaacggg ttgcattatt ggacgtgccc tctacgaggg acgattgaac ttacccgagg
     3181 tccttgccat cgctgaggat aaaggatcct gtcagtaaat agatacggtt agaaataaag
     3241 gtcgcttagc tatcgtgccc caatggtggg gcaggttttt tcggaatcgc atgtggaccc
     3301 tggcagaatc ggttattgag gacaggtttc tatggctctt tacgaagtga aaaaaatccg
     3361 gaatatcgca ttgtgtggtc acggttccgt tggtaaaacg actcttctcg atcgtttact
     3421 gaacttcacg ggggccgtca accgacccgc aagcgtcgat gatgggacga gcatctgcga
     3481 tttcgacgag gaagaaaagt cacaccgtta cacgattgag gcaaaggtca ccttttttga
     3541 ctatgacgga ctccactatc acgtgatcga tacgccgggt taccgggatt tcatcgggca
     3601 ggtcatcggg gcctttcgcg cggtggattt ggccgcgatt gtgattaacg ctcagtcggg
     3661 tgtcgaggtc aacacgcggc gagtttttca ggaggcagaa aaagctggaa tcccccgatt
     3721 gatcatcatt aacaagctcg acgcggaaaa tattgacttc gaaaatctct tggcaggtat
     3781 tcgagagacc ttcggcgatc ggtgcgtccc gctcaatgtc cccattggcc ttggtgctga
     3841 ttttcggggt gttgccacga ccctaaaggc cgagtcatcg gttcccggcg cggtcattga
     3901 ccccagttcg atcagtcagt ctttgatgga atcgattgtt gtggcggatg aggaggtatt
     3961 aagccgttat tttgaaggcc agatgccaac ggacgaggag atttcccaac tgctgggtcg
     4021 cgccatgatt aacgggtccg tcatcccgat cgtttgtgtt tcggccaaga caggggtagg
     4081 acttccggag cttcttaagg tattcgatct ttgtggcatt tcgcccgatc gaatcgtccg
     4141 gcgtgcccaa aaaccggaca gtggggaatg ggtcgagcta actgccgatc ccagtggccc
     4201 cctggttgcc caggtcttca agacgcgggt tgatcccttc gtgcaaaaga tcagcttttt
     4261 gcgggcgtac tcgggaacag tgaggaccaa cgcgacagtt tcggtggtgg gcgaacgcaa
     4321 gggcctgaag ttggcccagt tgttccatgt gcaggcttca gagatgatcc ccatcgagga
     4381 ggccgggcct ggcgcgatcg ttgccgtaac caaggtcgaa gaacttaaga cggggatgag
     4441 catcggggat ctcgtgatgc cacctatcga attccccacg cctatgaccg gcttggcaat
     4501 cacccccaag agccgcgggg acgaacaacg gctctctggc gccttagcga agatttgtca
     4561 ggaagacccc acctgtcggc tcgaccgtga cccccaaacc aaggagttgg tgatcacggg
     4621 aatgagcgag ttgcacctcc aggtgatccg cgaacgcctg aaacggcgtg acaaggtgga
     4681 agtggatgtc aaagagccga agattcccta tcgcgaaaca atccaagccc cggcggaagg
     4741 aatgtaccgc cataagaagc aaaccggcgg tcgtgggcag tttggcgagg tccacatccg
     4801 catgtttcct ttcccgcggg gcaccaaggt ggaggagttc gccacaaaag aacggttccc
     4861 cgaaatgaaa gaagtccatt atcacgagga atcaaacttc ctctggatcg actcgatcgt
     4921 gggcgggacc attcccagca actttatgcc cgccgtggag aaaggcttct tggaaagaat
     4981 cagccgtggc gtcatcgccg gctatccggt tcaggacgtc tgtgtggaag tcttcttcgg
     5041 caaacaccac ccggtggata gctccgagca agccttcaaa accgctgcct cgatggtctt
     5101 ccgcaatgtt ttccaacagg caaaaccggc cttgttggaa ccgattgtga agatcgaggt
     5161 aacagtcccg gcagacaagg tcggtgatat taacagcgat ctttcgggaa ggcgcggtcg
     5221 ggtccttggg atgacctcgg ctggaggtgg attccaaaca atcgtagccg aagtcccact
     5281 ggccgaggtg atcacgtacg cccgggcctt gtcgagcatc accggtggcc agggtagcta
     5341 caccttggaa tttagccact acgacatcgt cccgggaaat atccaaaagg aaattatcga
     5401 aaaggccaaa ctcgaagagg aagaggaaga ataaggtttt tagtccccca caaagcgaaa
     5461 gaaaaacagt cgggcggaaa cggttccatc gtttccgcct tttttgttgg gttaatgtca
     5521 ataaccctca acgcctgaat actgttggca atctcagctc gattgcagaa gacggctctg
     5581 acgacagcga agccctcagc ccactgggct aaggacacgt gcggacaatg attcaatcat
     5641 atgttgggtc ttttgcaaga acgactgtaa tggcgcagcc ccgaggtgct ggtgcagtag
     5701 ttccagagac tcccacttct cgataacggt gatgacatta tcacggaccg gtggctggac
     5761 cggcaaacct gcggctgtct caagggcagc cccatattcc aagcacccgg gctcctgtcg
     5821 caccgccggg gcgatcttct gaaactccgc aagcacggca tcgcgttgtc ccggtttggt
     5881 gacgatttcc gcaatcacgt gaatcatgaa cacatcctct cgtcgttcga agttaacaca
     5941 gttgcacaac ttacgggaga tggcattcag aaagacagta cttgcccagg acctccgcag
     6001 taggatcgac accgagaccc gcatctgacc agaaggaaac cgtcagtcgg ccattctgat
     6061 cccctctcac tgtttggagg ggctcggcga cgtcttccac gaaggcctcc gcagtgtccc
     6121 aataatcggg cggatagcag aaattggaga gagacgccag tgcaagggca gctctcacag
     6181 agatggttgt tcccgggtac aggcccaaat aacaaggcac gcagtgctga tgggccgcat
     6241 cgtgaatggc cttggccggt gtgagccccc caacccgaat cgggttgaca ttgagatact
     6301 tcgcgctgtg tagctcaagg gccatatcgg cttgggcagg agtcgtaatg gactcgtcca
     6361 ggcaaacagg ggtc
//
LOCUS       DAOPTR010000063         6301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_10152_length_6301_cov_3.135126, whole genome shotgun sequence.
ACCESSION   DAOPTR010000063 DAOPTR010000000
VERSION     DAOPTR010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6301
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10152_length_6301_cov_3.135126"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="PKI05_02140"
     CDS             <1..859
                     /locus_tag="PKI05_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51026.1"
                     /translation="WTLIPVSLSNENGPAVDAGLSTPYFGKLQQGSAGVFVRQVAELS
                     FKRGVTAAMQDGLNTDIAMDEKLLSGGDFAHRARLYWDVAVVQAEYHVFLQYSAILSE
                     LFPTVLARWNEPGAAEEMLQLQAFRRTVDAGLQDIHLKMLDRGSDLIALNSKSDNAHP
                     VAITLPYVGEYDTRWDQIRTVLPEPTRTAAMRVVSRIPATYAALCQNALALNAADQAR
                     VFAVGRFLNKQERIGPVLSALHFEHEAWLGFIELTGQYNQLIAEYVSLVRPDLDQKSF
                     LSAIGITQR"
     gene            1000..1998
                     /locus_tag="PKI05_02145"
     CDS             1000..1998
                     /locus_tag="PKI05_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HOA51027.1"
                     /translation="MTIGHLRICPPLFQAPMAGYTNYAFRQLLRSLGGVGLLCTEMVS
                     ARGFLELDRRYQRLPDRLWGVRDEPRPIAVQIWDNDPHKLAMVAERLVEDFDVSCLDI
                     NFGCPAEQITRGVACGAYLLRQPDDIGEIVSAVVKRVSPVPVTAKIRLGWSDQEINAP
                     AVAEVIQQAGGAAVFVHGRTAQQRYRGTADWSQIAAVKKRVQRIPVIGNGDIRSAQQA
                     VTAMREFGVDGVMIGRVALARPWIFREAAALLAGLEPPPEPPAEEQYEMLLELYRQLE
                     SYFGSVKAGILMRSFASRFVTARRGAKRFRVLIHSSKSSADLLAHLRDFFLHNPES"
     gene            complement(2057..2749)
                     /locus_tag="PKI05_02150"
     CDS             complement(2057..2749)
                     /locus_tag="PKI05_02150"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011120671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOA51028.1"
                     /translation="MSGLTIAIGDIHGYDVALRAVLDQIGPSGSDTVITLGDYVDRGP
                     NSREVLEILIELQGQVHLVPILGNHDEMMLSIWQGDHHLFDDWLHYGGAATLESYGLT
                     TLDGVPEHHIRFLQECRTYLETPTHIFLHANYIENRPLSEQDVFTLRWESLRQRLPGP
                     HRSGKKAIVGHTAQRNFEILDLGYLVCIDTCCYGGGWLTALDVESGQVWQADAQGRTR
                     KWVLDSFNTSPG"
     gene            complement(2825..3787)
                     /locus_tag="PKI05_02155"
     CDS             complement(2825..3787)
                     /locus_tag="PKI05_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOA51029.1"
                     /translation="MNDTVTWGLLGAVLVLLIIATVVVQLLRRSRPEVINSEIVESFS
                     GRLRAWWLLFAGLAGAIVLGKTATVVFFGLISFWALREFITLTPTRPSDHRALFWVFV
                     LCTPAQYVLVGYAQYDLFAIIIPVYALLFLHTRIAFSNDPVRFLERTAKIQMGLLICV
                     YCLSYAPALLTTLELGNDAYNLRLLFFLVFITQLSDALQFAWSQLPSRHVVVPNINPT
                     RTWEGLLGGSASVTLIGAMLWWALPFNSIWITAGLSLVISLVAFAGGITMSAIKRDRG
                     VKDYGTLVAGHTGVLDRIDSLCFAAPVFFHLTQIYLALQPAASA"
     gene            complement(3882..5414)
                     /locus_tag="PKI05_02160"
     CDS             complement(3882..5414)
                     /locus_tag="PKI05_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOA51030.1"
                     /translation="MSVFVHRQASESAVVQRPRRHFWLICCVFWAATCPWLGQPILGE
                     TEEEAVQHAKYLSIAFRSAARKVLPAVVTIKTATSKEGQEAGRGGPRLPWEEFPKPSE
                     PGFQNEIPRNPDLGMGTGVIIDPKGIIITNRHVVEGGDVVTVRLVDGREFPVIDKKSD
                     ELSDLAILKIEAAEDLPAARLGDSDKLDIGDWVIAIGNPFELEGTVSAGIVSAKGRTL
                     RSIGRVRFIQTDAAINPGNSGGPLVNLDGEVVGINTAIFSRSGGYQGVGFAIPINLVK
                     WVSSELLAHGRVRRAYLGVTLEDVLLPRKAVSGEERQSLGGVVVRQVIRETPADRAGL
                     RRNDVILSFDGVDVSDVASLQEIVERASLDVPHKVRVLRDGTSIELEIQLQESLSERE
                     LAERLKQQNESQDQPRRKRVYDKELGLMLMDFDPALAPDTGVRVTHGVVVIHADPGRP
                     GARAGLSTGMVIVKVNDRVINNLAEFQEAIKAADSKAGIKLEVVSRLGRQILNLGVGE
                     KK"
     gene            5840..>6301
                     /locus_tag="PKI05_02165"
     CDS             5840..>6301
                     /locus_tag="PKI05_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51031.1"
                     /translation="MKTNYRRRAFLMGSSALAIGAMIPWRFGVLNAATVEKLAEGAPH
                     AEKLGWRLGCQAYSFNRFTFYEAVEKTAAVGLRYIEAYPGQRLSQEKPDVKTDHNLSV
                     QDRLEVKKRLADAGVTLVNYGVVRLSADEGENRKVFDFAKDMGIETIVSEPP"
BASE COUNT         1524 a         1745 c         1641 g         1391 t
ORIGIN      
        1 gtggacactc attccggtat ccttatcgaa cgaaaacggt ccagccgttg atgcgggcct
       61 aagcacaccc tactttggca aattgcaaca aggctccgct ggagtttttg tgcgacaagt
      121 tgcggaactc agtttcaagc gaggggtgac ggccgccatg caggatgggc tgaataccga
      181 tatcgccatg gacgaaaaac tgctgtccgg gggagatttc gcccaccggg ctaggcttta
      241 ctgggacgtt gccgtcgtcc aggctgaata ccacgttttt ctccagtatt cagcaattct
      301 cagcgaactc tttccaactg tcttggcccg atggaatgaa cccggtgccg cagaagagat
      361 gttacaactc caggcatttc gccgcacggt ggatgccggt cttcaggata tccacctcaa
      421 gatgctggac agaggctccg acctgatcgc gcttaactcg aagtccgaca atgcccatcc
      481 cgtagcgatt acacttcctt acgttggaga atacgatacc agatgggacc agattcggac
      541 agtccttccg gaaccgaccc gcactgccgc catgcgggtg gtttcccgaa tcccagccac
      601 atacgcggcc ttatgtcaaa acgctttagc actgaatgcc gcagaccagg cgcgggtttt
      661 tgccgtagga cgttttctca acaagcagga gcgaattggt cccgtcttga gcgcccttca
      721 cttcgagcac gaagcctggt taggctttat tgaactaacg ggccaataca accaactcat
      781 cgccgagtat gtttccctgg tacgacctga tttggaccaa aagagtttcc tttctgccat
      841 cgggatcaca caacgttaaa tccgccgggt gttgcgcgag gtgactctcg aatgcaagag
      901 tctttcgcaa acaatcctca atctgaactg tctacaggtg aatctcattc tcctgctggc
      961 gggaacgagt ggcgtctaac ttcaagggta ccgacacagg tgacgatcgg ccacttacgc
     1021 atatgcccac ctctgtttca ggccccgatg gcgggatata ccaactatgc atttcgccaa
     1081 ttactacgtt cacttggcgg agtgggactg ctctgcaccg agatggttag tgcccgcggc
     1141 tttttggagc tggatcgtcg ctatcagcgg ctcccggacc gattgtgggg tgtccgagat
     1201 gaaccgcggc cgattgcggt tcagatctgg gataacgatc ctcataagct ggccatggtg
     1261 gccgagcgtc tcgtcgagga tttcgatgta tcgtgtttgg acatcaattt tggttgtcct
     1321 gcggagcaaa tcacccgggg ggtggcgtgt ggggcttatc tgcttcgtca gccagacgac
     1381 attggcgaaa tcgtatccgc cgtggttaaa cgagtatccc ctgtgccggt cacggccaag
     1441 attcgacttg gttggagcga tcaagagatc aacgcacccg cggtcgccga agttatccag
     1501 caagccggtg gcgccgccgt gtttgttcat ggtcgcacgg cacaacaaag ataccgcgga
     1561 actgcggatt ggtcacagat cgctgccgtc aaaaagcgtg tccagcggat tcctgttatc
     1621 ggtaatggcg acattcgttc cgcccaacaa gcggtaacgg ccatgaggga attcggtgtt
     1681 gatggcgtca tgatcggtcg tgtggctttg gcacgtccct ggatctttcg cgaagccgcc
     1741 gcccttttgg caggcttgga accaccgccc gagccacctg ccgaagaaca gtacgaaatg
     1801 ttactggaac tttaccgcca gctcgaaagc tatttcggga gcgtgaaggc aggaatcctc
     1861 atgcggagct ttgccagtcg ctttgttaca gcgcgacgtg gtgctaaacg gtttcgggta
     1921 cttattcata gctcaaagtc atcagcagac ctgcttgccc acttgcggga tttctttttg
     1981 cacaatcctg aaagctagga cgtatcccag caataacgga actgccaatc gcatcccgaa
     2041 gaatccctct tggctatcaa ccggggctgg tgttgaaaga gtccaagacc catttccgtg
     2101 ttcggccttg agcgtcagct tgccaaacct gtcccgactc cacatcaagg gctgtgagcc
     2161 acccaccgcc gtagcagcat gtatcaatac acacgagata accgaggtca aggatttcga
     2221 agttccgctg ggccgtatgt ccaacaatgg ccttttttcc cgatcgatga ggccccggaa
     2281 gccgttgccg caaagattcc caacgaagtg tgaatacgtc ctgttcactt agcggtcgat
     2341 tttcaatgta gtttgcgtgg agaaagatgt gggtgggtgt ttctaagtag gtgcggcatt
     2401 cctgcaaaaa acgaatgtga tgttcgggaa caccatctaa tgttgtcaaa ccgtaagatt
     2461 cgagcgtcgc cgcacctccg taatgcaacc agtcatcaaa aaggtgatga tctccttgcc
     2521 aaatggaaag catcatctcg tcatggttac ctagaatggg aacaagatga acttggccct
     2581 gcaactcaat gagaatttca agaacctccc ggctgtttgg accacgatcg acataatcgc
     2641 ccagcgtgat gaccgtatca ctacctgaag gaccaatctg atccaacact gcacgaagtg
     2701 cgacgtcgta cccgtggata tcgcctatgg ctatagtgag tccactcatt gtcgtttctg
     2761 tgtgatttct aatgcctctg ccgtaaggaa tgagcacccc aagaggttac caagagaccg
     2821 aggcctatgc ggatgctgcc ggttgcaatg ccagataaat ctgcgtgagg tggaagaaga
     2881 ccggagcggc aaaacagagc gagtcaattc ggtcaaggac ccctgtatgt ccggccacga
     2941 gagtcccata gtccttaaca ccacgatccc gtttgatcgc ggacatcgta ataccgccgg
     3001 caaaggccac taaggagatt accaaggaca gtccggcggt aatccagatt gaattaaagg
     3061 gcaaggccca ccacagcatg gcgccgatta gggttacact agccgatcct cccaaaagtc
     3121 cctcccacgt ccgcgtggga ttgatgttcg gcaccaccac atgtcggcta ggaagttggg
     3181 accaggcaaa ctgaagggca tcgctcagct gggtaataaa cactaaaaaa aagaggagtc
     3241 gaaggttgta ggcatcgttt ccgagttcca atgtggtgag aagggctggg gcatagctta
     3301 agcagtacac gcaaatgaga aggcccatct gaatctttgc cgtccgttcc aaaaatcgga
     3361 caggatcgtt ggagaatgca atgcgcgtat ggagaaagag taatgcgtaa acggggatga
     3421 taatggcaaa caaatcgtat tgcgcgtacc ccaccagaac atactgggcg ggcgtgcaaa
     3481 gaacaaagac ccaaaaaagc gctcgatggt cgctgggccg cgttggtgtc agggtgatga
     3541 attcccgcaa agcccaaaag gaaatgagcc caaaaaagac gacggtagcg gtctttccga
     3601 ggacaatggc cccagcgagc ccggcaaaca acagccacca cgcccgcagt cgccctgaaa
     3661 agctttcaac gatttctgaa ttgatgactt ccggccgact gcggcgaaga agttggacga
     3721 caactgtggc aataataagt agaacaagaa cggcgccaag aagtccccat gtgactgtgt
     3781 cattcatgga cgcaaacacc tcgcccaaac gctagggacg ggtttttcct tgcctaatgt
     3841 tctaggcctc gaggccaaag gtccctatca cccccatgct gttatttttt ctccccgaca
     3901 cccaaattga gaatctgacg gcccagccga cttaccactt cgagtttgat acccgctttg
     3961 gaatcagccg ctttgatggc ctcttggaat tcagccaagt tgttgattac gcgatcattg
     4021 accttgacga tcaccatgcc cgtggataga ccggcgcggg caccaggcct ccccggatcg
     4081 gcgtgaatga caaccacacc atgggtgacg cggacaccgg tatccggcgc aagagcagga
     4141 tcgaaatcca tcagcatcag gcccagttct ttatcataaa ccctcttgcg tcggggttga
     4201 tcctgcgact cattttgttg cttcaatcgc tctgccaact ctcgctccga aagagattcc
     4261 tgcaactgaa tctccagctc aatggaagtt ccatcgcgaa ggacacgaac cttgtggggc
     4321 acgtccaggg aggcgcgctc cacaatctcc tgaagactgg ccacatcgct tacatcgaca
     4381 ccatcgaagc tcaagatgac atcgttccgc ctgagccccg ccctgtccgc tggtgtctcg
     4441 cgaatcacct gcctcaccac gactcccccc aaactctggc gttcttctcc acttaccgcc
     4501 ttgcgaggaa gcaacacatc ctcaagcgtt acacccagat atgcccgccg aacacgaccg
     4561 tgagctaaca gctcggaact gacccacttg acaagattga tcggaatagc aaaaccaacg
     4621 ccctgatacc caccactacg cgaaaatatt gccgtgttga taccgacgac ctcgccatcc
     4681 aaattcacca ggggcccacc cgaattgccg ggattaatcg cggcgtcggt ctgaatgaaa
     4741 cgaacgcgtc caattgaacg caacgtccgg cctttggcgc tgacaatccc agcactgacc
     4801 gtcccttcca attcaaacgg attaccaata gctatcaccc agtcgccaat gtctaacttg
     4861 tcagaatcgc caagtcgcgc tgccggcaaa tcctccgcgg cctcaatttt taggatcgcg
     4921 agatcgctca actcatccga cttcttgtca ataaccggga actcacggcc atcgaccaga
     4981 cgtaccgtga caacatcacc accttcgacc acgtggcggt tggtaatgat gatacccttg
     5041 gggtcaatga tgacaccggt tcccatgccc aaatctgggt ttcggggaat ctcattttga
     5101 aaacccggtt ccgagggttt cggaaattcc tcccacggca accgaggccc accacgccct
     5161 gcctcttgtc cctctttaga cgtggctgtc ttgatcgtca caaccgcagg cagaactttt
     5221 cgggcggcac tacgaaaggc aatcgatagg tacttggcgt gttgaaccgc ctcttcctcg
     5281 gtctctccta aaatgggctg ccccagccat ggacacgtag ccgcccaaaa aacacagcaa
     5341 atcagccaga aatggcgtct tggtcgctga acgacagctg attcgctcgc ttggcgatgc
     5401 acaaaaacgg acataatcca atccttctga tgaaagtata aaaccgttcg cttgccgact
     5461 tcttttcctt cgacactttt ggccatcgaa aacccgccca ttctgcaagt ttatgccatt
     5521 aacccgggaa gaatcaacga agtacctctt gatgaggtaa gttttggcgg aataaagcgc
     5581 aagattgata cgttgtgcac gccgggaagg caccaccccg aggggtcggg gcgtcttgat
     5641 agttagaacc gcgagttccg ttcccgctgc catcgttgca cttcttcaat gatgaggcta
     5701 cgatgaatgg ctgaaatgct gggctattgg gctcgggagc aatttcaagc gagaacaacg
     5761 gcgaaggcac gggcaagtgc ttcgctgtcg cggctggtgt ggtttgttgc ttgcagcgat
     5821 catgttcgga ggttacacca tgaaaactaa ttaccggcgt cgtgcatttc tgatgggttc
     5881 ctcggctttg gcgatcgggg ccatgatccc atggcgtttt ggcgttctga acgccgcgac
     5941 cgtcgagaaa ttggccgaag gtgcccctca cgccgaaaag ctcggatggc gattggggtg
     6001 tcaagcttac agctttaacc ggttcacgtt ttacgaagca gttgaaaaga ccgcagccgt
     6061 ggggcttcgc tacatcgaag catatcccgg ccagcggctc tctcaagaga agccggacgt
     6121 gaaaaccgat cataacctga gtgtccagga tcgtttggaa gttaagaagc gattggccga
     6181 tgcaggtgtc accctagtga attacggagt ggtgcggctt tccgcggacg agggtgagaa
     6241 tcgaaaagtg ttcgatttcg ccaaagacat gggaatcgaa accattgtct cagagccacc
     6301 g
//
LOCUS       DAOPTR010000064         6290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_10179_length_6290_cov_3.316279, whole genome shotgun sequence.
ACCESSION   DAOPTR010000064 DAOPTR010000000
VERSION     DAOPTR010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10179_length_6290_cov_3.316279"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="PKI05_02170"
     CDS             complement(<1..843)
                     /locus_tag="PKI05_02170"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007337679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOA51032.1"
                     /translation="MRWNFLGKLWITLGVVGVFGWFDAGSAIRAESRTATPPSATVQQ
                     VGPKEAAFRKQLDAWINLLEQIREIRVKYPNANAAEMAQLREKYEQLLNEAEKQLPSL
                     IAAAEAAYDERPQTEGDLVDLLLEVALDRSEQGPFQDGLALAEKLIKNKVTSPVVYLA
                     AGRAAFALEDFTKAAGYFKKISEAGIQDEQVKAMEDAANFYAQQFPLEQQKRQAEAKA
                     DDLPRVLLKTTKGDILLELFENEAPNTVANFITLVEQGFYNGLTFHRVIPGFVAQGGC
                     PKGDG"
     gene            1089..1868
                     /locus_tag="PKI05_02175"
     CDS             1089..1868
                     /locus_tag="PKI05_02175"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOA51033.1"
                     /translation="MAKKHRVPELWIGTSGWTYDDWNGVFYPPDVKASARLDFYVQQF
                     NAVELNATFYRYPTANMITSWNRRMPETFHLAVKASRRITHLKRLRDCAAELTEFAVR
                     ISELRPLRVILWQLPPSMRPDLTLLSAFLEGLSRSILSGVRHALEFRHKEWWNDEVRE
                     LLCKYRVAFVAVSHPSLPSQIVPTTDFLYIRFHGLGRQLYRYDYSEAELHDWAKRVAE
                     VCCVADVSNVYTFFNNDFHAYAVKNAQTFRELLRGELRTAG"
     gene            2098..3156
                     /locus_tag="PKI05_02180"
     CDS             2098..3156
                     /locus_tag="PKI05_02180"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOA51034.1"
                     /translation="MNWETGLSPISTSIVLITVVALLLGLVTRRFVVYRDRHGGHEGG
                     TEGIVSRLGRFIYGLGLTLGDLKRDVARQGLALDQARGELADRASGTLDLGTILSTLA
                     FIANENRRLESRLQAAESQLQEQSRQLEKQWMEVRTDPLTGLANRRVFDLELSRRLRE
                     YRTHGTSFALALYDIDHFKQINDSGGHKTGDEILVHVARAMQSAAPEGALIARIGGEE
                     FAALLPDRGLSETLALAERIRVGPGTAFDGAPALSTTLSCGVAVCQTSDTETSLFERA
                     DRALYAAKRSGRNAMFYDSGDRSVRYTKLGAGPTACVPGLHECSEDEAAIAWYDDPEA
                     RALVDVLRRKLSDLRILG"
     gene            complement(3195..3734)
                     /locus_tag="PKI05_02185"
     CDS             complement(3195..3734)
                     /locus_tag="PKI05_02185"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOA51035.1"
                     /translation="MNRPKLQDPDPAITSATCRSAEITYRYSVQESDRLAVVNMCRET
                     GFFHEGEVAVAEELVVERLAKGPSSGYEFIFAERNGTVVGYVCFGPIACTQSSWDLYW
                     IVVSPHMQRMGLGGALLRETEQAVKHRGGTRIYIETSGREQYRPTRNFYEKHGYTVAA
                     VLTDFYAPGDDKIVYVKVL"
     gene            complement(3876..4982)
                     /locus_tag="PKI05_02190"
     CDS             complement(3876..4982)
                     /locus_tag="PKI05_02190"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOA51036.1"
                     /translation="MRRTVVLASLGLLAVFLVLGMVRGLSAQQPDVIHQPYLLDTGLI
                     SRSISFENPTGAPGEGGKAASHLGVGRKGSPSRPIKPGETIQLCDIEGPGTIRHIWLT
                     TEPVPENLRSLVIRAYWEGQEHPSIECPLGDFFGFAHGKVMPYFSAVHSVGPKAGMNI
                     WLPMPFTKRARITLTNETQGQRTPPIYYQIDYTLGDKHPEDVGRLHVLFRRENPTTEK
                     KDFELLPQRKNKGRFIGVVIGVRNLHPGQWWGEGEIKAYMDGDQEFPTICGTGSEDYV
                     GLSWGMQQVPFFYQGCSLNEKNFVSMYRWHLPDPIAWQKECRITIQQIGWNKGLVETQ
                     DDWCCATFWYEPTPSAPLPEMPDVAARIADIWTD"
     gene            complement(5165..6088)
                     /locus_tag="PKI05_02195"
     CDS             complement(5165..6088)
                     /locus_tag="PKI05_02195"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOA51037.1"
                     /translation="MGQLSKRIISGTLLAIVALVLCWLDATLATPAGIWLFPALILLV
                     VLSGNEIITLCRGAGVCPVPWVVHLGNLTIISATWGMALFWRHWEAVHPDRLARSWDI
                     AAVSSMAAMIALAAVVFLAFTVEMQRYRRPGEANINVAGTVYAVCYIGLLASFLVQLR
                     MAFGIESLASLLMVVKFGDSGAFAVGKILGRHHMAPQLSPKKTIEGAIGAVIFGVIGS
                     LLTFYALLPIFGLVGESPSFFVCTVYGIILAIVGMIGDLAESLIKRDAQQKDSSQLLP
                     GLGGALDLIDSSLMAAPVAYVFWLFGWLPLH"
BASE COUNT         1454 a         1725 c         1699 g         1412 t
ORIGIN      
        1 gccgtcaccc ttgggacatc ccccttgagc cacgaaaccg gggatgacac ggtggaacgt
       61 tagcccgttg taaaacccct gctccacaag ggtgatgaaa ttggccaccg tgttgggggc
      121 ttcgttttcg aagagctcca gcaaaatgtc acccttggtc gttttcaaaa ggactcgcgg
      181 cagatcgtcg gcctttgcct cggcctgtcg cttttgctgt tcgagcggaa actgttgagc
      241 ataaaagttt gcggcatctt ccatcgcttt cacctgttcg tcttgaattc ctgcttcact
      301 gattttcttg aaatagcctg cggccttggt gaaatcctcc aacgcgaagg ccgcccgtcc
      361 agccgcgaga taaacaaccg gcgacgtgac cttgttctta atcaacttct cggccagggc
      421 cagaccatct tgaaaaggcc cttgctcact tcggtccagg gccacctcca aaaggagatc
      481 gacaagatcg ccctcggtct gggggcgttc atcataggcg gcctccgccg ctgcaatcaa
      541 agagggcaac tgtttttcag cctcattcaa taactgctca tacttctctc gcaattgggc
      601 catttccgcg gcattggcgt ttggatactt cacgcggatt tcccgaatct gctcaagcag
      661 gttaatccac gcgtcgagtt gcttacggaa agctgcctct ttcgggccaa cttgttggac
      721 agtcgcgctg ggcggggtgg ccgttctaga ttcggcccgg attgccgaac cggcgtcaaa
      781 ccatccaaaa acacctacca cacccagcgt gatccacaat tttccaagga aattccatcg
      841 catcacgtat cgtcctttcg tcaaataggg acaggaaacg aaacggggca agcttgactt
      901 tccccgagag ttcacttttc attgtagcag aaatccccag aaggggttgc gagcggaaga
      961 taatacggga caaacgtgtt gcgcgtccaa agattggcgt caagaagggc aagtggaaac
     1021 agtttcgcga gatttgctcc cgctttaacg ggacggcaat tctggagtcg ttagagtgag
     1081 cgtcgtcaat ggccaagaaa catcgtgtcc ccgagttgtg gattggaact tctggttgga
     1141 cttacgacga ctggaacgga gttttttatc cgccggacgt gaaggcatcg gcccgactcg
     1201 atttttacgt ccagcaattc aacgccgttg agctgaacgc gaccttttac cgctatccca
     1261 cggcaaacat gattacctcg tggaaccggc ggatgccgga aacgttccac ctcgcggtta
     1321 aggcctcccg gcgaatcacg catcttaagc gacttcgcga ttgtgccgca gaactgaccg
     1381 aatttgccgt gaggatcagt gaattgcgtc ccctgcgggt cattctttgg caactgccgc
     1441 cgtcaatgag gccagatctc accttacttt cggcatttct cgaggggctc agtcgatcga
     1501 tcctctccgg agttcgccat gccctggagt tccgtcacaa agagtggtgg aatgacgagg
     1561 tcagggaact gctgtgcaag tatcgggtgg cgtttgtggc ggttagccat cccagtttgc
     1621 catcacaaat cgtcccgacc acggatttct tgtacattcg tttccatggg ttgggacggc
     1681 agttgtatcg ctacgattat tcggaagccg agttgcacga ttgggccaag cgcgttgccg
     1741 aggtatgttg tgttgccgat gttagcaacg tgtatacttt ttttaacaat gattttcacg
     1801 cttacgcggt aaaaaatgcc caaacttttc gtgagcttct caggggagaa ttaagaacgg
     1861 caggataagc ctggacaatt cttttggcat ttcttgcaaa actcgcaaac ttgtcgcagc
     1921 ggacgtcttt ctattgttca caaacgttgg acaacggtct cctcaaaaat gtaggttttc
     1981 ataggaggac atgaaacggc aagaaaccgt ttcgggggaa ggcttttctt ggaaccttcc
     2041 ccgatgtgac gtgttttagc tacactgaga tcgctcaatg tggatcaagg tgtccaaatg
     2101 aactgggaaa caggattgtc tcccatcagc accagcattg ttcttatcac cgtggtagcc
     2161 ttgctacttg ggttggtcac ccggcgattc gtggtttacc gggatcgtca tggaggccat
     2221 gaaggaggaa ccgagggcat cgtcagcaga ttgggccgat tcatttatgg tttagggcta
     2281 acgttgggtg accttaaaag ggatgtcgcg cggcagggtt tggcgctcga ccaggcgagg
     2341 ggcgaacttg ccgaccgggc cagcggaaca ctggatttag gaacgatcct cagtacatta
     2401 gccttcattg ccaatgagaa tcggcgatta gagagccgac ttcaagctgc ggagtcacaa
     2461 ttgcaggagc agtcaagaca acttgaaaaa caatggatgg aggtgcgaac tgatcccttg
     2521 accggtctgg ccaatcgcag ggttttcgac ctagaactta gccgcagatt gagggagtat
     2581 cgaacgcacg gcacatcatt cgccctcgcc ctttatgaca ttgaccactt taagcagatc
     2641 aatgacagtg ggggacacaa gacgggcgat gagatccttg tccacgttgc ccgcgccatg
     2701 cagtcggcgg ccccagaggg tgctttgatc gctcggattg gcggtgagga gttcgcagcc
     2761 ctgttgcccg atcgtggact gagcgaaacc ctggccttgg cagagcggat ccgtgttggg
     2821 ccgggtacgg cctttgatgg tgctcccgcc ctatcaacga cgctcagttg tggtgtcgcc
     2881 gtgtgtcaga cgtcggatac agaaacgagt ctttttgaac gggctgatcg ggccctttat
     2941 gccgcaaaac gatcgggtcg taatgccatg ttctatgact ccggtgatcg aagtgtgcga
     3001 tacacaaaac tgggcgcagg ccccacggct tgcgtgccgg gcttacatga atgttcggaa
     3061 gatgaagcgg ccatagcctg gtatgacgac ccggaggcca gggccttggt tgacgtcttg
     3121 cggcgaaagc tgtcggactt gcggatttta gggtgaaaca atcaagcgag caggaccgca
     3181 acgggctacc ttttttacag gaccttcaca tagacgatct tgtcatcccc gggtgcgtaa
     3241 aagtctgtca agaccgcggc cacggtgtac ccgtgcttct cgtaaaagtt ccgcgttggc
     3301 cgatattgct cgcgtcccga tgtttcaatg taaatgcgtg tcccgccccg atgtttgacg
     3361 gcttgctctg tttctctcaa cagagcaccg cccaggccca ttcgctgcat gtgtggggat
     3421 acaacaatcc aatacaaatc ccaactcgat tgggtacaag cgataggacc aaagcagaca
     3481 tagcccacaa cggtgccatt tcgctcagcg aaaatgaatt cgtacccgct gcttggtcct
     3541 ttggcgagtc gctcgacgac cagctcctcg gcgacagcca cctcgccttc gtgaaagaag
     3601 ccggtctcgc ggcacatgtt aaccacggcc aagcggtcac tttcttgcac ggaataccgg
     3661 taggtaatct cggcagagcg gcacgtggcc gaggttattg cggggtcagg atcttgtaat
     3721 ttcggacgat tcatagaatc ctttctcggc taacatgttt cctgcgggga ttcaacgcga
     3781 ctgtggttcg atcgacctgt gcagacagac tccccaagtc ggcatacgcg tgccgtgtgc
     3841 cgacggttct gtgactggcc cccaagtccc acgaattaat cagtccagat atcggcaatt
     3901 ctggcagcga catctggcat ctcgggcagc ggagcactgg gcgttggctc ataccagaag
     3961 gtggcgcagc accaatcatc ctgggtttct acaaggcctt tgttccagcc aatttgctgg
     4021 atggtgattc ggcattcctt ctgccatgca atgggatcag gtaaatgcca acgatacatg
     4081 gagacaaagt tcttttcatt gagcgagcac ccctgataaa agaaaggcac ttgctgcatt
     4141 ccccatgaga ggcccacata gtcttcactg cccgtgccac aaatagtggg aaactcttgg
     4201 tcgccatcca tatacgcctt tatttcacct tcgccccacc attggccagg atggagattt
     4261 cgaaccccaa taaccactcc gatgaatcgt cccttgttct tccgctgggg cagtaactca
     4321 aagtccttct tttcagttgt agggttctct cggcggaaaa gcacgtgaag gcgtccgacg
     4381 tcttcggggt gcttatcgcc taaggtgtag tcgatctggt aatagatcgg cggtgttctt
     4441 tggccctggg tctcgtttgt cagagtgatc cgtgcccgct tggtaaaggg catcggcaac
     4501 caaatattca taccggcttt tggaccaacc gagtgcacgg cggaaaagta aggcatcacc
     4561 ttgccgtggg caaagccgaa aaagtcgccc aatggacatt caatactcgg gtgctcttgc
     4621 ccttcccaat acgcccgaat gacgagactc cgcaaatttt ccggcaccgg ttcggtggta
     4681 agccaaatgt ggcggatcgt accaggccct tcgatgtcgc acagttggat ggtctcgccc
     4741 ggcttgatcg gccgagaggg cgatcccttc cggcccaccc caaggtggct cgcagccttc
     4801 ccaccttcac cgggggcacc tgttgggttc tcgaaagaga tcgatcgcga aatcaaaccg
     4861 gtatccagca aatatggctg atggatcaca tccggctgct gcgcggaaag gccacggacc
     4921 atgccgagca caaggaaaac tgccaaaaga ccaagcgaag caaggactac cgttcgtcgc
     4981 atggtatcat ctcctattgt ttgcataacc atcccagact aaacctccaa agcctgcgca
     5041 aggttgctgt catttttgcg tggtaggccc caattggcaa tgacctcggc cacaaatcga
     5101 tgagccacca atcgctccag tgaccgcctt agagtgggac gtcactgcct gcgcgctctg
     5161 agtgtcaatg aagcggcagc cagccaaaca gccaaaagac gtaggccacc ggggcggcca
     5221 tcaatgagga atcgatcaaa tcgagcgctc cgcctaatcc tgggagtaac tgactagagt
     5281 ccttctgctg ggcatcgcgc ttgattaacg attcggccag gtcgccaatc attcccacga
     5341 tggccaggat aattccatac acggtgcaga cgaagaagga ggggctttcc ccaaccaatc
     5401 caaaaatggg taacaaagcg taaaaggtga gtaacgatcc gatcacaccg aagatgaccg
     5461 caccgatggc cccttcaatg gtcttcttgg ggctaagctg tggtgccatg tgatgccgac
     5521 ccagtatttt gccaaccgca aaggccccac tgtccccgaa tttgacgacc atcaacaaag
     5581 aggccaagga ttcgatgcca aaggccatgc gcaactgaac cagaaagctg gcgagtagcc
     5641 caatgtaaca cacggcgtaa actgtcccgg ccacgttgat attggcctcg cccgggcggc
     5701 gataccgctg catttccacg gtaaaggcca agaacacaac cgcagcaagg gcgatcatgg
     5761 cagccatact agagaccgcg gcgatatccc acgagcgagc cagccggtcg ggatggactg
     5821 cctcccagtg tcgccaaaac aatgccatgc cccacgttgc cgagattatc gtcaaattgc
     5881 ctagatgaac cacccaaggg acggggcaca ctccggcacc acggcataac gtgatgatct
     5941 cattaccgga gaggacaacc agaagaatta gggccggaaa aagccagatc cccgctggtg
     6001 ttgccaatgt cgcatctagc cagcataaca ccaatgcaac gatcgccagc agcgtgcctg
     6061 agatgattcg ttttgacaac tgacccatgg atggacttca ttcgcaagtg acaacacgag
     6121 cgcatgtaaa ccgtcatcgt gaccgatgcg gcagcttagg gcaagacccc attggctagg
     6181 cgtaccgaag gctgcccggt ggcgcattca gaatcctttg tcaaaccgtt ggcggcttag
     6241 agcctatccc acaacctcct ctctcccgtt cggcgggaga ggggccgggg
//
LOCUS       DAOPTR010000065         6290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_10180_length_6290_cov_3.135044, whole genome shotgun sequence.
ACCESSION   DAOPTR010000065 DAOPTR010000000
VERSION     DAOPTR010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10180_length_6290_cov_3.135044"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="PKI05_02200"
     CDS             complement(<1..1233)
                     /locus_tag="PKI05_02200"
                     /inference="COORDINATES: protein motif:HMM:NF036965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOA51038.1"
                     /translation="MELTRYQVTVLPLGRTIEVLAGSRFVDIARTLGLAISLPCGGEG
                     VCGKCRVWVRSGMAEPTAADRRHLTESELEQGQRLACQLAVRSDVVIEIPETLLLRAS
                     HQILTKTGEVRLEKDTPLVTKRTVNLTETPSATDLVAIVRHALPQVGLPLCVLQDLSQ
                     QLLEIPSTRLAVLAEGCLLALESPEEASPTLGVAVDLGTTTLAASLLDMTTGAELAVC
                     GRLNPQTEFGDDVISRIQFASETPWGTQRLQHAVVSAINEMIEELIGQAGVDRQMIYQ
                     VVCAGNTTMQHLFCGLSPQHLGRFPFRATTLEAQRFFASEVGLNVHPRGRVYVFPAIG
                     GFVGGDTVAGIVTTGLDQGRSPALLVDLGTNGEIVLWDGQVLRAAATAAGPAFEGARI
                     KCGMRATEGAIERVWWDET"
     gene            1715..2410
                     /locus_tag="PKI05_02205"
     CDS             1715..2410
                     /locus_tag="PKI05_02205"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOA51039.1"
                     /translation="MLQKKWLALAAAGLVVAGVIVYQLRAQQVTTPTAPVRSATLVRN
                     IAVVDVEYIFKNHLRFKQEMSVLRSEATAVEQWAQREQEAIKSLAEQLKDHQPGTATY
                     NQIDDTLVKRQKDFEAEIIKKRKEFLLREAKLHYATYQEIVNEIQYLAAANGIAVVLR
                     FNGQQVDPNSPDDILRYINQLIVYQAPELDLTPLVLQRLNARAQSTTSSASSTSSPVV
                     PGQGNFPRPGTLR"
     gene            2624..3499
                     /gene="lpxC"
                     /locus_tag="PKI05_02210"
     CDS             2624..3499
                     /gene="lpxC"
                     /locus_tag="PKI05_02210"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOA51040.1"
                     /translation="MLSLRPQQTIARAVAVEGFGYWSGTDVRVEFRPAPPNSGIVFVR
                     EDLRHRPRIPARLEYRMETPRRSSLEFSGARVEMVEHVMAALGGLQIDNCEISVTAAE
                     MPGCDGSALPFVEALLQAGIIKQSAPRAVRVLTRVVRVEDRNSWFEARPGIANECFMR
                     YLLDYGPDGPIGRQSFGLALTPESFCRELAPCRTFMLKEEAEWLLSQGLGRRTTPRDV
                     LVFDADGPIDNQLRFPDECVRHKLLDMVGDLALCGCDFVGRFSAYRSGHRLNAEMGRN
                     LLNQFPLVERIRSCA"
     gene            3568..4641
                     /locus_tag="PKI05_02215"
     CDS             3568..4641
                     /locus_tag="PKI05_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA51041.1"
                     /translation="MSASSGGNGNPIPVGVIGAGRLGTFHARKLARLRDVRLVGVADP
                     LVDRREAVAKECRCSAFADYRDLLPHCAAVVVAVPTSLHHRTAMDCLEAGVHVFVEKP
                     ITETPQQAHQLWSTAQRKNLVLQVGHIERFNPAYRAVQPLLGDIQLIEASRCGPFSFR
                     VTDVGVVMDLMIHDLDLILAMVRKPVLRTDSVGVSLLGRHEDLAIARIEFEDGPVALL
                     KASRIEQEPIRTMRIWHRAGQAIVDFARRTASVCEYPEEIRSGAFRIEELSPQELNSL
                     KETFTSRFFPWRQLEIASYDPLEAELRNFIDAVRTGTLPLVTGGDGLAAVQLAERILD
                     GIRARESLIRPIVPIPRSLPKAA"
     gene            5872..>6290
                     /locus_tag="PKI05_02220"
     CDS             5872..>6290
                     /locus_tag="PKI05_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOA51042.1"
                     /translation="MLTRCCLRFPAWSISVLCVLVWATGLSATFGLNEDLRETPIVRA
                     IRSARPAVVNIRGEKSLITTAGNSTRPDAGRRVNGMGTGVVIDERGYIITNHHVVDGV
                     REIEVTLHDGTRYIGKLVARDPKTDLAIIKINPDQPL"
BASE COUNT         1402 a         1823 c         1673 g         1392 t
ORIGIN      
        1 ggtttcgtcc caccacaccc tttcgatggc gccctctgtc gcccgcatgc cacacttgat
       61 ccgggccccc tcgaatgccg gtccggcggc cgtggctgcc gcacgcaaaa cttgcccatc
      121 ccagaggacg atttcaccat tcgtgccgag atcgacaagt agggcagggg accgaccctg
      181 gtccaagccg gtggtcacaa tcccggcaac agtatctcca ccgacaaatc cgccgatggc
      241 tggaaacaca taaacccggc cacggggatg aacattcaac ccgacctcgc tggcaaagaa
      301 acgttgagct tccaacgtgg ttgcccgaaa gggaaatcgc cccagatgct ggggagatag
      361 tccacaaaaa aggtgctgca tggtagtgtt gccggcacaa acgacttgat aaatcatttg
      421 tcgatcgacc ccggcctggc caatcagctc ttcaatcatc tcattgatgg cagaaaccac
      481 ggcatgttga aggcgttgag taccccaagg tgtctcactg gcaaattgga tccgagaaat
      541 gacgtcatcc ccaaattccg tctgtggatt tagtcgccca catacggcca gttccgctcc
      601 agttgtcata tccaaaaggg aagcggccaa ggtggtcgtc cccaagtcaa cagccacacc
      661 taaggtcgga gaagcctcct cggggctttc cagcgctaaa agacaaccct ccgcaagcac
      721 ggctaaccgc gttgacggaa tttccaatag ttgctgagac aggtcctgca acacgcagag
      781 cggtaacccc acctggggca aggcatgacg aacgatcgcc accaaatcgg ttgccgacgg
      841 ggtctcggtt aggttgaccg tccgttttgt caccagtggg gtgtcctttt ccaatcggac
      901 ctcgcctgtt ttggtcaaaa tctggtgtga tgcccgcaaa agcagcgtct cggggatctc
      961 aataaccacg tctgatcgca cagccaattg acaggccagg cgctgtcctt gctcaagctc
     1021 cgactccgtc aaatgacgcc ggtcggcagc tgtcggctcc gccattccgg accgcaccca
     1081 gacccggcat ttgccgcaca caccctctcc gccacagggc agactaatgg ccaatcccaa
     1141 cgtgcgggca atatccacaa atcgtgaccc ggccagaacc tcgatcgtcc gccccagggg
     1201 taaaaccgta acttggtatc gagtcaattc cacgtttgcc aaccttaata atgccaattc
     1261 gaattctgtc gttggaagta aaaccgcaaa cccttagctt accatgaatg gaccaagccg
     1321 gtcatttccc gccaggtggc actctgcttc tggggttaca ctttgcctgc atcgtgctaa
     1381 acggaattgc cctttacgaa tgttcctaga atccccacaa tgggctgccg tttgagccca
     1441 gttcggtgga agaatggcgt ctgtcggaag tgagttaacg tgagacggct cgccaccctg
     1501 ttttcttcga gaaagctaag ggaaggcagg catcaaaaac gctggagggt cgagcccacc
     1561 ataacgggtg gtacctaatg acactatccg gtaaaccttt ttgtcacatg ccatcggtcg
     1621 ttgcccaatt tgatctcgac gcagagtcag gaattcgata cgctttggaa aattggtcag
     1681 gagcgaccat tcaccacgat caaggaggat ttcgatgttg cagaagaaat ggttggcatt
     1741 ggcagcagca gggcttgtcg ttgcgggcgt gatcgtttat cagttgcgtg cccaacaggt
     1801 aacaactcca acggcacctg tgcggtcagc aacgcttgtc cgaaacattg ccgtggtcga
     1861 tgtggagtac attttcaaga accacctgcg atttaaacag gagatgagtg tcctgcggtc
     1921 tgaggcgacg gcggtcgaac agtgggcaca gcgcgaacag gaggccatca agtcgttggc
     1981 agaacaactc aaggatcatc aaccggggac tgcaacctat aaccaaatcg atgatacgct
     2041 tgtcaagcgc caaaaagact ttgaggctga gatcatcaag aaacgtaaag agtttctctt
     2101 gcgagaggcg aaactccact acgccaccta ccaagagatt gtcaacgaga ttcaatattt
     2161 ggctgctgcc aacggaatcg ccgtggttct ccgattcaat ggtcagcagg ttgaccccaa
     2221 ttcacctgat gacatcttgc ggtacatcaa tcaactgatt gtttaccagg ccccggaact
     2281 ggatttgacg cccctcgtgc tgcaacgact gaacgcaagg gcacaatcga ccacatcgtc
     2341 ggcgtcaagc acatcgtcgc cagttgttcc cggacagggg aactttcctc gtcccggaac
     2401 gctccgctga gcgaacttga actgcggtgt gcggaggtgc aaaaaaggcg tttctttgat
     2461 ctttcgcccc gcgcgttcca cgattgagaa attttagctt gtacgagaga tgtgcctaac
     2521 ctgggggcta ccaaacggcc gccaggttag gcaaaatcgg cagggtattg ttgacctatg
     2581 tgacgtcctt aaaacatctt cgatcgacag acgccagacg ttaatgcttt ctctgagacc
     2641 ccaacaaacc atcgcacgtg ctgttgccgt tgaaggtttc ggatattggt ccggaacgga
     2701 cgtccgtgtg gaattccggc cagcccctcc caattcgggg attgtcttcg tacgggagga
     2761 ccttcgacat cgcccacgca tcccagcacg gctcgaatat cgtatggaaa cgccccgccg
     2821 cagttccctg gagtttagtg gtgccagggt cgaaatggtc gaacacgtga tggccgcgct
     2881 cggcggacta caaatcgaca attgtgaaat ttcggtcacg gccgccgaaa tgccaggctg
     2941 tgatggctcg gccctaccgt tcgtggaggc actgcttcag gctgggatca tcaagcaatc
     3001 agccccgcgg gcggtgcgtg tcctgacccg agttgtcagg gtcgaggatc gaaatagctg
     3061 gttcgaggcc cgccccggca tcgctaatga gtgtttcatg cgatacctgc tcgattacgg
     3121 ccctgatggc cccattggcc gtcagagctt cggcctagcc ttgacgcccg agtcattttg
     3181 tcgagaactt gctccctgcc ggacgttcat gcttaaagag gaggccgagt ggcttctctc
     3241 acaaggccta ggacgacgaa cgacaccacg agatgtgttg gtgttcgacg ccgatggccc
     3301 catcgacaat cagcttcgat ttcccgatga gtgtgtacga cacaaacttt tggatatggt
     3361 tggggatttg gccctttgcg gctgcgactt tgtggggcgt ttttcagcgt accgaagtgg
     3421 acaccggctc aatgccgaga tgggccggaa cctcctcaac cagtttcctc tcgtggagcg
     3481 gattcgatcc tgtgcctgag gtctagaagg ctgcctaacc ccacatcata actgcaccta
     3541 caaaacagga tgaaaagcac agattggttg tcagcatcat ctggaggaaa tggcaatccc
     3601 atccccgtgg gcgtgatcgg ggccggacgc ctcggcacgt ttcacgcacg aaagctcgca
     3661 aggcttcgcg acgtcaggct ggttggcgta gccgatccgc tcgtcgatcg tcgcgaggcc
     3721 gtggctaaag aatgccggtg ttccgccttc gccgattatc gtgatctctt accccactgt
     3781 gctgccgtcg tggtggcggt gcccacatct cttcaccacc gcacagcaat ggattgcctt
     3841 gaggcagggg tccacgtgtt tgtggaaaaa ccgatcaccg agacacccca acaggcccat
     3901 caactgtgga gcacagccca acgaaagaat cttgttttgc aagttgggca tatcgagcgg
     3961 ttcaatccgg cctaccgggc ggtgcagcca ctcttgggtg acattcaact gatcgaagcc
     4021 tcccggtgcg gtcccttcag tttccgagtt accgatgtgg gcgttgtgat ggatctgatg
     4081 atccatgatc tcgacctgat tctggcgatg gtcaggaagc ccgttctgcg gaccgactcc
     4141 gtgggtgtgt ccctcttggg cagacatgag gacctggcca tagcccgaat cgaattcgaa
     4201 gatggcccgg ttgccctcct gaaggcctcg cggatcgagc aagaaccaat ccgcactatg
     4261 cgtatctggc acagggcagg gcaagcgatc gtggactttg cgcgacgaac ggcttcggtg
     4321 tgcgaatatc cggaagagat ccggtctggc gccttccgta tcgaggaact tagcccacaa
     4381 gagctgaata gtctcaaaga gacatttacg tcacgattct tcccatggcg acagcttgaa
     4441 atcgcgtctt acgatcccct tgaggccgaa ttgcgcaact ttattgacgc cgtgcgaacg
     4501 ggaacccttc ccctagttac gggcggagac ggcctagccg ccgtgcaact ggcggaaagg
     4561 attctcgacg gcattagggc tcgcgaatcg cttatacgtc ccatcgttcc catcccacgc
     4621 agtttaccaa aggcagcgta aaccaaatag caagcaggaa ggcactacat tcgggctact
     4681 gtcagccttt tcaatcccta agtgccttgg aactctcaac cgctcttctt taaatcgccc
     4741 atactaacca gtcggaagta tcctcttctt tcgtgcaagc cttgtaaccg gaatattcct
     4801 caacgtttga caggacgcaa gacgatgaca cctgagggtg tccgaaatcg ttgtgttgtc
     4861 ttgtgtttct cgattggaca agaccgattc actcaggtgg ctcatttttg ctctcggtcg
     4921 cttctgattt gtttgatact cctggcgggc tgcaagtgtt atcgctctcg caccgctagc
     4981 tgccggcctt gttcaccctg cctgggtgcg tcggaaacct gccggggatc gccacgtttt
     5041 cgctctggaa acggttttcc ggtaactccc ccgcccgatg cggacttagg accagagggt
     5101 tttccagaat ccttcaccgc ctccatcggg tcatcgcatg caaagttctt tccccttccc
     5161 acccggcctg tttttcgcgt caatgccata ggggagcagt cccgcgagac atcagctaaa
     5221 gagacggacc taaattcttc cccctccgta cccgaagtga ttcctacgcc gccgcctctg
     5281 ccgcccgggg agttcaacga tcaaacacga actgttacac cccggatgtg gatgtatgag
     5341 tccagccgtt ggcgatccag cactgccagt gacaaagccg gtagggatga cgagccttct
     5401 cccaatgtgt cggacgtgtc gtttgctcca agccaccagg tttcctggct gtttcagggg
     5461 cctttttcta gcgccatcgc gcatacgggg gaagaacctc gtgtgattct cccaagccag
     5521 ccccccaccg ccacgtcagc ccgccgaaca accgggatgc aaaggtaagt cctagcatcc
     5581 tccgttgggg ctgcccgttc tactcgggca ttcttagagc acgtcgatcg tgcggaacga
     5641 gcagtttcca ctttgtgttc ccaggcaagc actcattgca tgagttaacc gatggcaatg
     5701 tgggcaaacg aaacaaagct tagagacttt gtgggttacg agcacgtgta tcaaggatgc
     5761 tgattctctc gccgatgcac acttttgagc cttcttccca cacttgaaga aggaaagtcg
     5821 cactgttggc acggttttcc acgacccttt agcaacggcc tgccgtagaa catgctcaca
     5881 agatgctgcc tgcgatttcc ggcctggtct atctcggttc tctgtgtttt agtttgggca
     5941 acggggctct ccgcaacatt cggtcttaat gaagacctgc gggagacccc tatcgttcgg
     6001 gccatccgaa gtgcccgtcc cgcagtggtg aacatccgcg gtgagaaatc gctcatcact
     6061 accgctggca attccacacg gcccgatgca ggccgccgtg tcaacggtat gggaaccggc
     6121 gtggtcatcg acgagcgtgg ttatatcatc acgaaccacc atgtcgtgga cggtgttcga
     6181 gaaatcgagg taacgcttca tgacggaacg cggtacatcg gcaaacttgt cgcccgggac
     6241 cccaaaaccg atcttgccat catcaagatc aatcccgatc agcctctgca
//
LOCUS       DAOPTR010000066         6256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_10253_length_6256_cov_3.457991, whole genome shotgun sequence.
ACCESSION   DAOPTR010000066 DAOPTR010000000
VERSION     DAOPTR010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10253_length_6256_cov_3.457991"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1953
                     /locus_tag="PKI05_02225"
     CDS             <1..1953
                     /locus_tag="PKI05_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HOA51043.1"
                     /translation="TRFYFSTRLPKTVKFEIDLQDPQDQYARLFADEVVRTVNDLTLP
                     RYGLGNYVKPSPHNPPTAEEAKVLADLSRAGTRLKGFCRTNLFKRLESSGHSFILSVQ
                     RHILRNYIFLHAIENGLPLPIGTQDMGLLDTSANDEDSQLWDPAADDDENQSPDKSVA
                     IQSQLPNESDLQRRAADLYQTFASQFHRRFKWLRADLFDKALADDLRKDAQALIKAIQ
                     RAGTWDPQRDTKLQSLVQLLTKQHPDEKVLVFSQFADTVRYLEEQLKASGLTAVAAVT
                     SATSNPTEIAYRFSPVSNEKREFVNPADELRVLLTTDVLSEGQNLQDCSIIVNYDLPW
                     AIIRLIQRAGRVDRIGQQSDTILCYSFLPADGVERVIRLRARVRQRLRENAEVVGADE
                     AFFEGEADDQPLRDLYTEKAGILDGEEDTEVDLASYAYQIWKNAIDRNPQLEKIIPAM
                     PNVVYSAKPLPPNADRREGVLVYVRTGEGNDALTWIDTDGNSVTESQFAILKAAECTP
                     DTPALPRAEDHHKLVEKAIELIVKTEKSVGGQLGRPSGARFRTYERLKAYAQAIKGTL
                     LESADLLKAIEDIYKYPLLQSAIDTLNRQLKSGISDEDLAALVIRLRQEGRLCEVAKD
                     GEAGRPQLICSLGLRRPDSHAARSCV"
     gene            1971..5282
                     /locus_tag="PKI05_02230"
     CDS             1971..5282
                     /locus_tag="PKI05_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51044.1"
                     /translation="MKFDVAAVRNCLKSFDFQTLFREHLGWDNYHAQLDVPVGENTFH
                     LTAVAQKRGFVAFVCPSIPDRATRLKIDHHVTKSVREHFVIYFDKAAGQQVWQWVRRE
                     PGKPLASREYRFHVSQSGDPLIQRLNQIAVSIEEEEQITVVDVANRARAAFDVDKVTK
                     KFYDRFKAEHADFLKLIKGIKYEEDLQWYTSLMLNRLMFVYFIQKKGFLDGDTDYLRN
                     RLKRVQEIKGKGKFHTFYRYFLLRLFHEGLGKSPEERKLDPDFERLLGKVPFLDGGFF
                     EVHELEQRWEEIDIPDEAFEKLFDFFDQYRWHLDERPLRADNEINPDVVGYIFEKYVN
                     QKQMGAYYTKEDITEYISKNTIIPFLFDAARKKCPIAFKPDSAVWRLLREDPDRYIYP
                     AVRHGVIRPDGSVVPESDLPEFVQKGMHDPNARMFDKRYNLEQAPAGDPIRLVTETWR
                     EYVYRRNRCLEIREKLQKGEIHEINDLITLNLDIWQFARDAIVNCEDPELLRAFWHAV
                     EKITVLDPTCGSGAFLFAALRILETLYGDCLERMERFVEDLAGKKHPPEQFSDFKEVL
                     ERVAEHPNERYFILKSIIINNLFGVDIMEEAVEICKLRLFLKLAAQVEKIDQIEPLPD
                     IDFNIRAGNTLVGFASLEQVRKSQEGRLGFAAEEARRIEEEAELVERAFQQFRAQQTT
                     HGGRVTHRDKQQLRRRLEKLTDKLDRFLAGEYGVNLDKPEEFEAWKASHQPFHWFAEF
                     YGIMRNGGFDVIIGNPPYVEYSQRSVGYRVLENEYLTFSSRNLYAFVYERSKHLDHSH
                     ARLGIIVQISSVSTPQMRTMAFEIRHGQNFVWVSNYATRPSCLFEGVTMNLTILLSKT
                     GVAEQDTPNHLWSTKYIRWNPESRHIVFPTITYVDVPPDVYLFDFAVPKLTSACEITM
                     LRRLLRERQTLEALLEISKARTSQCLHYRTAGGRYFKIFSDRPLGTESTSNKRKFLKE
                     EYNARAAIAVLSSSLWWWYYTLHFDMYNCKDYMIYGFRFDFNKSGAAQELAELGSALV
                     ESLFKNAKRKTQQYATTGARRQLLFRPSASKAIIDEIDKVLAKHYGFTDEELDFIINY
                     DIKYRMGDDAGVDEE"
     gene            5279..5401
                     /locus_tag="PKI05_02235"
     CDS             5279..5401
                     /locus_tag="PKI05_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51045.1"
                     /translation="MTRRLRVVNDVLQPIRVERGEGACPASLEFLSNRILEALI"
     gene            5606..6031
                     /locus_tag="PKI05_02240"
     CDS             5606..6031
                     /locus_tag="PKI05_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51046.1"
                     /translation="MTIYSLGNPHRAEQDLRTKAVIKGYSPREFAQLVGVAPTYLWQA
                     QQGNIQPARTGPLKGHYSRAKRSCVMALPTHPVWLGILPLWESPVDRATPKCRLRMAG
                     FCVGKLGARSKTRLPVAGPRFFPDLLWPLTPDERPAPVS"
BASE COUNT         1441 a         1819 c         1754 g         1242 t
ORIGIN      
        1 acgcgatttt acttctcgac gcgcctgcct aagacggtca agttcgagat cgacctacag
       61 gacccacaag accagtatgc gcgcctattc gcggacgagg tcgtgagaac ggtgaacgac
      121 ctgaccttgc cgcgctatgg actagggaac tacgtgaagc cttcgcccca caatcctcct
      181 acagcggaag aggcgaaggt gctggccgac ctctctcgcg ctgggacgcg cctcaagggc
      241 ttttgccgta ccaacttgtt caaacgcctg gaaagcagcg gccactcgtt catcctgtcg
      301 gtgcaacgcc acatcctccg aaactacatc ttcctgcatg ccattgaaaa cggcctgccg
      361 ctccccatcg ggacgcagga catggggctg cttgacacct cggcgaatga cgaggacagc
      421 caactttggg acccggctgc cgacgatgat gagaaccaat cgccagataa atccgttgcg
      481 atacaaagcc agcttccaaa tgagagtgac ctgcagaggc gggccgcgga tctctaccaa
      541 actttcgcat cccagttcca tcgccgcttc aagtggttgc gcgcggatct cttcgataaa
      601 gccctcgccg acgacttacg caaggatgcc caggcgctaa taaaggcgat ccaacgtgcc
      661 gggacatggg atccccagcg ggacaccaaa ttgcagtccc tcgtccagct tctcaccaag
      721 cagcaccccg acgaaaaggt actcgttttc tcacaattcg ccgacacggt caggtaccta
      781 gaggagcagc taaaggcaag tggtctgacg gccgtcgcag ccgtgacgag cgcaacatcc
      841 aatcccacgg agattgcgta ccgattcagc cctgtcagca acgagaaacg ggagtttgtc
      901 aacccggctg acgaactgcg cgttctattg acgacagacg ttttgagtga ggggcagaac
      961 ctgcaggact gctccatcat cgtcaactac gacctccctt gggccattat ccggctgatc
     1021 cagcgggctg ggcgtgtaga tcgaatcggc cagcagtcag atacgattct gtgctactcc
     1081 tttctccccg cggacggcgt ggaacgagtc attcgcctcc gggcacgagt acgtcaacgg
     1141 cttcgcgaga acgcggaggt ggtcggtgcc gacgaggcct ttttcgaggg agaggcagac
     1201 gatcagcccc tgcgcgatct ctacaccgag aaggccggca tcctagatgg cgaggaggac
     1261 acggaagttg accttgcatc ctacgcctac caaatctgga agaacgctat cgacagaaac
     1321 ccgcaactcg agaaaatcat cccggccatg cccaacgtgg tctattccgc caagccgctt
     1381 ccaccgaacg ctgaccgtcg cgagggagtg ctggtctacg tccggaccgg ggaagggaat
     1441 gacgcactga cctggattga cacggacggg aatagcgtaa cggaatcgca gttcgccatt
     1501 ctcaaggccg ccgaatgcac gccggacacc ccagctctgc cacgggccga ggaccatcac
     1561 aaactggttg aaaaggcaat cgagctcatc gttaaaaccg agaagtccgt cggtggccag
     1621 ttggggcgcc cttcgggtgc gcggttccgc acctacgagc gcctcaaggc ctatgcccag
     1681 gccatcaagg gaacgctctt ggagtccgcc gatctgctaa aggccattga ggacatctac
     1741 aaataccccc ttctccaatc cgcgatcgat acgctcaacc gccagctcaa gagcggcatc
     1801 tccgacgagg atttggcagc ccttgtcatc aggttgcgac aggagggccg cttgtgcgag
     1861 gtggcaaagg acggagaagc cggaaggcct caactcattt gttccctcgg actacgcaga
     1921 ccggacagtc atgccgcccg gtcgtgtgtg taagtgcgga ggacaagctc atgaaattcg
     1981 atgttgctgc cgtcaggaac tgcctaaagt cctttgactt ccaaactctt ttccgggaac
     2041 acctggggtg ggataactat catgcccaac tcgacgttcc cgttggcgag aatacgttcc
     2101 acctcaccgc ggtcgcacag aagcgaggtt ttgtcgcgtt cgtctgtccc tccatccccg
     2161 accgcgccac acgcctcaag atcgaccacc acgtgaccaa atctgtccgc gagcactttg
     2221 tcatctattt cgacaaggca gccggacaac aggtttggca gtgggtccgc cgggaaccgg
     2281 ggaagcccct ggccagccgg gagtatcggt tccatgtctc tcagtcgggc gaccccctaa
     2341 tccagcgact caaccagatc gcggtgagca tagaggagga ggaacaaatc accgtcgtgg
     2401 acgtagccaa ccgcgcccgg gccgcgttcg acgtggacaa ggttaccaaa aagttctacg
     2461 accggttcaa ggccgaacac gcggacttcc tcaagctcat taagggaatc aaatacgagg
     2521 aggatcttca gtggtacacg tccctcatgc tcaaccgcct gatgttcgtg tacttcatcc
     2581 aaaagaaagg atttctggac ggcgatacgg actatctccg caaccgcctc aagagggtgc
     2641 aggagatcaa ggggaagggc aagtttcaca cgttctaccg ctacttcctg ctacgcctct
     2701 tccatgaggg cctcggcaag tctccggagg agcgcaagct ggatcccgac tttgagaggc
     2761 tgctcggcaa ggtgccattt ctggacggcg gattctttga ggtacacgaa ctcgaacaac
     2821 ggtgggaaga gattgacatt cccgacgaag cgtttgaaaa gctatttgat ttcttcgatc
     2881 agtaccgttg gcacctcgac gagcgtcccc tgcgggccga caacgagatc aaccccgacg
     2941 tggtcggcta cattttcgag aagtatgtca accagaagca gatgggggcc tactacacca
     3001 aggaggatat caccgagtac atcagcaaga acaccattat ccccttcctg ttcgacgccg
     3061 ccagaaagaa gtgccctatc gccttcaagc ccgattcagc cgtctggcga ctgctccgcg
     3121 aggatcccga ccgttacatc tacccggccg ttcgtcatgg cgtcattcgg cccgacggat
     3181 cggttgtgcc agaaagtgac ctgcccgagt tcgtccaaaa aggcatgcat gaccccaatg
     3241 ctcgcatgtt cgacaagcgg tataaccttg agcaagcgcc cgccggggat ccgatccgtc
     3301 tcgtcaccga gacgtggcgc gagtacgtct atcgccggaa tcgttgttta gaaatccggg
     3361 agaaacttca gaagggggaa attcacgaga tcaacgacct gattacgctc aacctcgaca
     3421 tctggcagtt tgcccgggac gcaatcgtga actgcgaaga cccggagctt ctccgggcgt
     3481 tctggcacgc cgtcgagaag attaccgtcc tcgacccgac ctgcggttcc ggcgcgttcc
     3541 tctttgccgc cctgcgaatt ctggagaccc tctacggaga ctgtctggag cggatggaac
     3601 gcttcgtgga ggacctcgct ggtaagaagc accctcccga gcagttcagt gacttcaagg
     3661 aggtcctaga gcgggttgct gagcacccca acgagcgcta ctttatcctc aagtccatca
     3721 tcattaataa ccttttcggc gtcgatatca tggaggaggc ggtcgagatc tgcaaactgc
     3781 ggctgttcct taagctggca gcccaggtcg agaagattga ccagatcgag cctctcccgg
     3841 atattgattt caacatccgc gccgggaaca cgctggttgg gtttgcatcc cttgagcagg
     3901 ttcgcaagtc ccaggagggc aggctcggct tcgccgcgga ggaggcaaga cggatcgagg
     3961 aggaggccga actggtcgaa cgggcgttcc agcagttccg cgcccagcag acgacgcatg
     4021 gcggccgggt cacccatcgt gacaagcaac agttgcggcg tcgcttggag aagctgacgg
     4081 acaagctcga ccgtttcctg gccggggagt acggcgtcaa cttggacaag cctgaggagt
     4141 tcgaagcctg gaaggccagc caccagccct tccactggtt cgccgagttc tacggcatta
     4201 tgcgcaatgg cggcttcgat gtgatcatcg ggaatccgcc gtatgtggag tactcgcaac
     4261 gatcggttgg ataccgggtt ctcgagaacg agtaccttac cttctcaagc agaaatctgt
     4321 atgccttcgt atatgaacgg tcgaaacatt tggatcactc acacgctcgg cttggaataa
     4381 tcgtgcaaat cagctcagta tcgactcctc agatgcgtac aatggcattc gaaataaggc
     4441 acggtcaaaa ctttgtgtgg gtgtccaact acgcaactcg gccatcctgc ttgtttgaag
     4501 gcgtcacgat gaatctaacc atcctgcttt cgaaaactgg agtcgccgag caagatacgc
     4561 caaaccattt atggagcact aagtacatca gatggaatcc cgaaagccga cacattgtat
     4621 ttcccacgat aacttatgtc gacgtccctc cggatgtgta cttgttcgat tttgcggtcc
     4681 caaaactcac cagcgcttgt gagatcacaa tgcttcgtcg gctcttgcgc gagcggcaga
     4741 ccctcgaggc cttacttgag atctcaaagg ctcgcacttc gcagtgcctg cactatcgga
     4801 ctgccggtgg gcgctacttc aagatttttt cggatcgccc tttgggtacc gaaagtactt
     4861 ccaacaaaag gaaattcctc aaagaggagt ataacgcaag agcggcaatt gcggttctaa
     4921 gctctagtct gtggtggtgg tactataccc ttcacttcga catgtacaac tgcaaagact
     4981 acatgattta cggttttcgc tttgatttca acaagagtgg cgcggcacaa gagctggcgg
     5041 aattgggaag tgcactcgtt gaatcgcttt tcaaaaatgc aaaaagaaag acacaacagt
     5101 acgccacaac aggggcgcgg aggcagttgc tctttcgtcc ttcggcatcg aaggcgatca
     5161 tcgacgagat cgacaaggtc ctggcaaagc actacgggtt caccgacgaa gagttggatt
     5221 tcatcatcaa ctacgacatc aaataccgca tgggggacga cgccggggtg gacgaggaat
     5281 gacgaggcgg ctgcgtgtgg tcaacgatgt cctccagcca atccgcgtgg aacggggcga
     5341 gggcgcatgc ccggcttcgc tagaattcct ctcaaaccgg attctggaag ctcttatctg
     5401 aaaacaactt acgtcaatcc gaaaaaactc tggccacgtc cccggctata ttcccgccag
     5461 ccctagtgca ccccaatggc gaggcctatg ggccgggcga tcttctcgaa gtggacggcc
     5521 ggttgtccgt tgtcgctgag tggcttgaga aagttgccac cgacgatcag aaacagcaaa
     5581 cgattttcag ggaattcgta ggattatgac aatttattcc ctggggaatc cgcaccgggc
     5641 ggagcaagac ctccgcacga aggccgtcat caagggctac agcccgcggg aatttgccca
     5701 gttggtgggt gtcgctccaa cgtatctgtg gcaggcacaa caagggaata tccaacctgc
     5761 gaggaccggt ccgctcaaag ggcattactc ccgtgcgaaa aggagctgcg tcatggcttt
     5821 gccgacgcac ccggtctggc ttggaatcct cccgctttgg gaaagccctg ttgatcgagc
     5881 aaccccgaaa tgccgcttgc ggatggccgg gttttgcgtc gggaagctag gtgccagatc
     5941 aaaaactcgc ctgccggttg ccggtcctag gttcttcccc gaccttcttt ggccgttgac
     6001 ccccgacgaa cggccagccc ccgtgagctg actttctttc tctctatagc tcagttcggc
     6061 gcgaagctag cgggacgggc gaatgagcgc tcccggggaa acactgggtt gtacccagca
     6121 aaaaacgctt ggcggtgcgc gcgaaacaca tggagagaca ggccagtgcc gggtgactgg
     6181 gcgcggcgag agacaggcca atgccgggtg actgggcgcg gcgagggaca cgccaatgcc
     6241 gggtgactgg gcgcgg
//
LOCUS       DAOPTR010000067         6231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_10307_length_6231_cov_3.437824, whole genome shotgun sequence.
ACCESSION   DAOPTR010000067 DAOPTR010000000
VERSION     DAOPTR010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10307_length_6231_cov_3.437824"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PKI05_02245"
     CDS             complement(<1..654)
                     /locus_tag="PKI05_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOA51047.1"
                     /translation="MSQLHLNRYIRQVQCAKFGEESQQRLQTSRVFICGCGALGTTLA
                     NHLVRAGVGFVRLVDRDLVNLHNLHRQVLFDEADAAQSLPKAIAAVEHLKRINSEVSL
                     EAIVADVRPNNIDQFAFDVDLILDATDNFETRFLLNDYSLKHKIPWIFAACLSTQGQV
                     YPIIPGRTPCLRCLVPELPLPGSVPTCEMAGILGPVVGVVASIQAMEAMKILAKKFDS
                     "
     gene            complement(741..3725)
                     /gene="uvrA"
                     /locus_tag="PKI05_02250"
     CDS             complement(741..3725)
                     /gene="uvrA"
                     /locus_tag="PKI05_02250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOA51048.1"
                     /translation="MSSSDQSIIIRGARVHNLKGINLDIPKDRIVVICGPSGSGKTSL
                     AFDTLFVEGQRRYIESFSPRIRQRLDDLPPSEVDHISGLSPTVAITGRPSSRLRHATV
                     ATLAGVYHYLRLVFAHLGQVFCHRCGRPVRKWNSAAIENFASSLSASPLRIMLAFPLE
                     FSSAPSKAATLTSVLADLRASGWARVIVGNRIVNLTESSLAEVEKQLVGGESQRSEGF
                     DKLNDHRSVYVVADRLTIQEKKVPGRFRETLDRLENEGKDVVTLFIQENEPLATNSTA
                     GAQAVTMIIEGERWIRYDFPLALCCATCHIDYELPHPDLFNFNTAAGACETCQGLGTV
                     DSLEASQIFPDRHLSLKDGAAAPFRSASFSRFHLRLLEFAKRIRIPIDRPVGDLPQEY
                     IDWLLHGSPDDSSSCIEQIFRELERYRYKPQIKTFLSRWRVPRTCPNCQGSRLRIQSL
                     AVRLVPKTAMENFFGKGMTPPSPLDQTEKPLNIAEFCEKSIGDARAFLKTVVPNGLNP
                     GARLAVNEVLRRLQMLESLGLGYLTLSRPADTLSQGEFRRVLLGAALGSSLVNVLYIL
                     DEPSLGLHPNDIHRLRDVITLLRDRGNTVVVVEHDEALLKCADWVIELGPGAGERGGE
                     VVFQGTLEDLQKSETLTGEYLSARRQIKIDRTRRTPAGWIKLVGATGHNLKHVTAEFP
                     LNVLCVVTGVSGAGKSSLIEETLYPALCQRLFGTSSPALPFEDLIGERQISDVILVDQ
                     SPISKTSRSNPATILKVFDEIRALFAQTSEAKTRGLTPGHFSFNAGDGRCGRCLGEGR
                     LEIDMEFLPNMEITCPDCHGRRYRPDVLSVLYRGKSIADVLDLTIREAYFFFRGHATI
                     QSKLRRLMDVGLEYLRLGQATRTLSGGESQRLRLASYFTRSHKKATLFILNEPTTGLH
                     PHDIVQLLRCFESLLAYGHSLIVIEHNLHVMVAADYIIDLGPGPAEQGGSIVAQGPPE
                     EIARTPESVTGRYLLDLLPQMS"
     gene            complement(3836..5152)
                     /locus_tag="PKI05_02255"
     CDS             complement(3836..5152)
                     /locus_tag="PKI05_02255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOA51049.1"
                     /translation="MSGEQRRVVITGLGVICPIGTGATEIWQAVTNRQSGVSSPSCLP
                     TETLPIKAAGEARQFTGNIKDFGEMDKESQKNLRKSLKVMCRECQMGVAAAQLALNDA
                     SLTQGSFDPERVGTSFGSDYMVSSAEEFTEGIRACLDGNGVFEFHRWASDGMPRLFPL
                     WLLKYLPNMPASHVAIYNDLRGPSNSITMREASPAICVRDAMHAIQTDREDAAVVGVT
                     GTRLSLMKAMHAIQQEEVAIYDGEPQRACRPFDLHRSGMVPGEGAAAIILEELKTATR
                     RGASIYGEVLAASSAAVLERKCVARRREAMRQAMLNAIHEAGLKPTDIGFVMAHGLGT
                     QTCDVEEAQALSDVFGSRGVPIIAAKSYFGNLGAASGLVEMILGILALRHRQLFATLN
                     YETPDPACPVDVVTEPRPIEKMVFMNVNVTPQGQASAAIIAAYDGT"
     gene            5310..5516
                     /locus_tag="PKI05_02260"
     CDS             5310..5516
                     /locus_tag="PKI05_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009093499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HOA51050.1"
                     /translation="MADAVYKKIQVVGTSSESFSDAAAKAVSKVAETEKNLSWFEVVE
                     LRGAISDGKITQYQVTVNVGCRVS"
     gene            complement(5621..>6231)
                     /locus_tag="PKI05_02265"
     CDS             complement(5621..>6231)
                     /locus_tag="PKI05_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOA51051.1"
                     /translation="YGKDIVVVDCGLIFPDEDMPGIDLVIPDMTYLEKNAEKLRGILI
                     THGHADHIAGIEGVWRRWPEIEIVVGEQDAEKLTNPRLNLSADFGFPLTTPPPTKLVR
                     DGDQLTIAGLEILVREVPGHSAGHVVYVVNQATPPLAFVGDVIFAGSVGRTDFPGGSM
                     EQLAEGIRSKIYSLPEETELWPGHGEPTTVGRERRTNPFVRG"
BASE COUNT         1408 a         1732 c         1694 g         1397 t
ORIGIN      
        1 ggaatcgaat ttcttggcta atatcttcat tgcctccatg gcctggatgg atgccacgac
       61 accgacaacc ggtccgagta tgcccgccat ctcgcatgtt ggcaccgaac ccggcagcgg
      121 tagttcaggg accaagcacc gcaggcatgg tgtcctgccc ggaatgattg ggtaaacctg
      181 tccttgagtg ctcagacagg ccgcaaaaat ccacggaatt ttgtgtttga gtgaatagtc
      241 attgagtaaa aaccgtgtct caaaattgtc ggtggcgtca agaataaggt ccacatcgaa
      301 ggcaaactgg tcgatattgt tcggcctcac atcagctacg atagcttcca gcgagacttc
      361 cgagttgata cgcttcagat gttccacagc cgcgatcgcc ttgggcaagc tttgagcagc
      421 gtcagcctca tcaaaaagaa cctggcgatg caagttgtgg agattaacaa gatcgcgatc
      481 aacgagtctc acgaatccca ccccggcacg gacgaggtgg ttggctaacg tcgttccgag
      541 cgcaccgcaa ccacagataa acacccggct tgtttgcagg cgttgctgcg actcctcacc
      601 gaacttcgcg cattgaacct gacgaatata acgattcaag tgaagctgtg acataaaaga
      661 ttgtctcgtc gtttggcttt ccgccctctc ctccagcgtt gggcttaaag tgggcgctcc
      721 ctattgtgaa tcatacccag ctagctcatc tgaggaagaa gatctagcag ataacgaccg
      781 gttacagatt caggagtgcg tgcgatttct tccggtggtc cctgggcgac gatacttcca
      841 ccctgctcgg cgggacctgg tccaaggtcg atgatgtagt ccgccgcaac catgacgtgc
      901 aggttatgct cgatgacgat cagcgaatgc ccgtaggcaa gaagcgattc gaagcaacgg
      961 aggagctgca cgatatcgtg aggatgaaga cctgttgtcg gttcgttaag aatgaaaagg
     1021 gttgcctttt tgtgggagcg tgtaaagtag gacgccaggc gaagccgttg ggattctcca
     1081 ccggataggg tccgcgtggc ctgacccagc cgaagatatt ccagcccaac atccattagc
     1141 cgacgcagtt tggactggat cgtagcgtgg ccccgaaaaa agaaataggc ctcgcgaatg
     1201 gtcaagtcga gaacatcggc gatgcttttg ccgcggtaaa ggaccgaaag cacatccggt
     1261 cgatagcggc gaccgtggca atcggggcaa gtgatctcca tgttgggcaa gaactccata
     1321 tcgatctcaa ggcgtccctc tccgaggcat ctcccgcacc tgccatcacc cgcgttgaag
     1381 ctgaaatgcc ctggcgtcag ccctcgtgtc ttggcctcgc ttgtttgggc aaacagggcc
     1441 cttatttcat cgaagacctt gaggattgtc gcgggattgg aacgagatgt tttgcttatc
     1501 gggctttgat ccacgagaat aacgtccgag atctgtcgtt ctcctataag gtcctcaaag
     1561 gggagggcgg gagaacttgt tccaaacagt cgttggcaca gggcgggata aagggtttct
     1621 tcaatcaggc tgctctttcc tgctccactg acacccgtta cgacacaaag aacattgagc
     1681 gggaattctg cggtgacgtg cttcagattg tgtcccgtgg ccccaaccag tttgatccag
     1741 ccggcaggtg tgcgtctggt tctatcgatc ttgatttgtc gtctcgccga gagatactct
     1801 ccggtgaggg tctccgactt ttgcaaatcc tcgagtgtcc cctggaagac aacctcgcca
     1861 cctctttcac cagcacctgg acctaattcg ataacccaat ctgcgcactt aagcaaggct
     1921 tcatcgtgtt ccacaacgac caccgtattg ccgcggtcac gtaatagcgt aatcacatcg
     1981 cgtagccggt ggatgtcgtt gggatgaagt cccagcgaag gttcgtcgag aatgtaaaga
     2041 acattgacca gactggaacc cagagcggcg cccaacagga ccctccggaa ctcgccttgg
     2101 ctcagcgtgt cggccggacg gctcaacgtc aaataaccca gtccaagtga ttcaagcatc
     2161 tggagccgcc gcaaaacttc gttcacggcc agccgcgctc caggattaag cccattaggc
     2221 accaccgtct tcaagaacgc acgggcgtcg ccaattgact tttcgcagaa ctccgcaatg
     2281 ttcagaggtt tttccgtctg gtcgagcggt gagggtggcg tcattccctt gccgaaaaag
     2341 ttctccatcg ccgttttcgg gaccagtctc acggccaggg actggatacg taagcggctt
     2401 ccttgacaat tgggacaggt tcgtggaact cgccaacggc tcaagaaggt tttgatctga
     2461 ggcttgtagc ggtaacgttc aagctcacga aagatctgtt cgatgcacga cgaactgtcg
     2521 tctggcgagc cgtgcaacag ccagtcgatg tactcttgtg gcaagtcacc aacggggcgg
     2581 tcaatgggaa ttcggatcct cttggcgaat tccagaagcc tgagatgaaa tcgcgaaaag
     2641 ctcgccgacc gaaatggtgc agctgcccca tccttaaggc tgaggtggcg gtctgggaag
     2701 atctggctcg cctctaacga atcaacagtt cccaacccct gacacgtctc gcaggccccc
     2761 gctgccgtat tgaagttgaa taggtcggga tgcggcagtt cgtagtcaat gtggcatgtc
     2821 gcacaacata gcgccaacgg aaaatcgtat cgtatccacc gctccccttc gatgatcatc
     2881 gttacggctt gtgcacccgc tgtgctgttg gtcgcaaggg gttcattttc ctgaataaag
     2941 agcgtcacaa catccttgcc ctcgttctcc aatcgatcga gcgtctcgcg gaaacgaccc
     3001 ggtacctttt tctcctgaat agtcaaacga tcggcaacca cgtaaacgga ccgatggtcg
     3061 tttaacttgt caaaaccttc tgatctctgg gactcgccac ccacgagctg cttttcaact
     3121 tctgcgagag aagattccgt tagattgacg atgcgatttc caacgatgac acgcgcccag
     3181 cccgaagcac ggagatctgc caaaacggag gttaaagtgg ctgctttgga tggcgcgctg
     3241 ctaaactcta acggaaaggc aagcattatt cgcaaagggc ttgccgaaag actcgacgcg
     3301 aaattctcga ttgcggccga gttccatttt ctgacagggc ggccacatcg gtggcaaaac
     3361 acctgtccta aatgtgcgaa gaccaagcgg agatagtgat aaacaccggc caacgtggct
     3421 acagtggcat gacggagccg agaggaaggt cgccccgtga tggctacggt tggcgaaagg
     3481 cccgatatgt gatcaacttc tgacggcggt aggtcatcca atcgctgacg aatgcggggc
     3541 gagaaacttt cgatataccg acgttgccct tcgacgaata atgtgtcaaa ggcaaggctc
     3601 gtcttgccgc ttccactggg accgcagatg acaacgatcc tgtctttggg tatgtctaga
     3661 ttgatcccct tcaaattgtg gacgcgtgcc ccgcggatga tgatcgattg atccgacgag
     3721 ctcatggtca aggtgatgtt tcctgtcaac ccaagcgcag tctcctcggc tcgacaaagc
     3781 tcaacctacc cacttgagac cggcagtcaa aaaccttgtc ttcgttcact tagggttatg
     3841 taccatcata cgccgcgatg atggccgcag aggcttggcc ttggggagtg acattcacat
     3901 tcatgaacac cattttctcg attggccggg gctccgtgac aacatccacg ggacacgcgg
     3961 ggtccggggt ttcgtaattg agcgtggcaa acaactgccg atgccgcagc gcgaggatcc
     4021 cgaggatcat ctccacaagg ccactggctg ccccaagatt gccgaaatag cttttcgcag
     4081 caatgatggg cacaccgcgc gagccgaaga cgtcacttag cgcttgggcc tcttcgacat
     4141 cgcaagtttg agtacctaat ccatgggcca tcacgaatcc gatatctgtg ggcttcaatc
     4201 ccgcctcgtg aatggcgttg agcatagcct gacgcatggc ttctcgccgc cgggcgacac
     4261 atttcctctc caaaaccgca gcggaggagg cggcgaggac ctcgccgtaa atggaggccc
     4321 cgcgacgggt cgccgtcttc agttcctcga ggatgattgc ggcagcccct tcccccggta
     4381 ccatcccgct gcggtgaaga tcaaaaggcc gacacgcccg ttgcggttcc ccatcgtaaa
     4441 tggcaacttc ctcttgctga atcgcgtgca tggccttcat aaggctcaat cgagttcccg
     4501 tcaccccgac aacggccgca tcctctcggt cagtttggat ggcgtgcatt gcatcacgaa
     4561 cacaaatagc cggactggcc tcacgcatgg tgatggagtt actcgggcct cggaggtcgt
     4621 tatagatggc cacatggctt gccggcatat ttggcaagta cttaagaagc cagagaggaa
     4681 ataaacgggg catgccgtcg ctggcccagc ggtggaactc aaacaccccg ttcccgtcga
     4741 ggcaggcccg aatcccttcc gtaaactcct cggccgagct aaccatgtag tcgctgccga
     4801 agctggtccc gacccgctcg ggatcgaatg acccctgggt gagggacgca tcgttgaggg
     4861 ccaattgagc ggctgccaca cccatctggc actcccggca catgactttc aacgatttgc
     4921 ggagattttt ttgggactct ttgtccatct ccccaaaatc tttaatattt cccgtaaatt
     4981 gtctggcttc tccagccgct ttgatcggta atgtctccgt tggcaagcaa gacggggagg
     5041 agaccccgct ttgcctattc gtgacagctt gccaaatctc agttgcaccc gtcccaattg
     5101 ggcagatgac ccccaggccg gtgatcacca cacgacgttg ttccccactc atcgagacaa
     5161 cccttttcga ttcacgatcc tttttctatg cgggtgaacc ggttatctta tcaaagtagc
     5221 gcgcgaccgt cgagtcgctg tatttccgtg agcgtattag ttagcagtaa gttggatatc
     5281 aggcgaggtg ccacgaaagg gtgaaactta tggcagacgc tgtgtacaaa aaaattcaag
     5341 tggtaggtac ttcgagtgag tcgttcagtg atgccgcagc taaggccgtc agcaaagttg
     5401 ccgaaacgga gaaaaacctc tcctggtttg aagtggtcga gttgcgaggg gccatttcgg
     5461 atgggaagat tacgcaatac caagtaacgg tcaatgtggg ttgtcgcgtc agttagccca
     5521 ttttggggac gtcttacaga ctttgccggg aagcgggggc gacccacacc tcgcaatcac
     5581 attgtaaggg attagtcccc caaatagtgc gttccgaagc ttagccgcga acgaatgggt
     5641 tagttcgtcg ttcccgaccg accgtcgtgg gttccccgtg gccgggccaa agttctgttt
     5701 cttccggcag cgagtagatt ttgctccgga tgccctcggc caactgttcc atgctcccgc
     5761 ccgggaagtc ggtccgcccg acgcttcccg caaagatgac gtctccaacg aaggctaacg
     5821 gtggcgtggc ttgattgacg acgtacacca cgtgtccagc ggagtgcccg gggacctcac
     5881 ggacgaggat ctcgagacca gcgattgtta actgatcacc atcgcggacg agcttcgtcg
     5941 ggggtggggt cgtcagcggg aaaccgaagt cggccgagag gttgagccgg gggttggtca
     6001 gtttttcggc atcctgttct cccactacga tttcaatctc tggccagcgg cgccacaccc
     6061 cttcgatccc cgcaatgtga tcggcatggc catgcgtaat gagaatcccc cggagttttt
     6121 ccgcattttt ttcgagatac gtcatgtccg gaatcacaag gtcaatgccg ggcatatctt
     6181 catccggaaa aatcaggccg caatccacta ctacgatatc ctttccgtat t
//
LOCUS       DAOPTR010000068         6205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_10352_length_6205_cov_6.238211, whole genome shotgun sequence.
ACCESSION   DAOPTR010000068 DAOPTR010000000
VERSION     DAOPTR010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6205
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10352_length_6205_cov_6.238211"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..1552)
                     /locus_tag="PKI05_02270"
     CDS             complement(179..1552)
                     /locus_tag="PKI05_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51052.1"
                     /translation="MSQWQKKKPGDPLKIPAGAWNALLELLEQRDNFLAQLGKTGTID
                     TRIKIRNDTGADLGRYQVVRVSAPIVSPTDNLSEFLANPCFSAAIPDGEHNRIAIVQE
                     PIAAGAIGWAVAAGLTHAYVSGNESHGFAVPIAGDCTGFASAPSGPVAILWRETGTGL
                     RRCFVRVGNHPMDVRRLRLLAPLTHCGEADALLLTGTADEPPCDGQITVTVRDSLGVT
                     SPSGLPAGAFVWAVLMPDAGKFEVISYGFGCCEPPSQSDSDQSDSDQSDSGPSESSIP
                     SESSAPSESSGPSESSQSQPSESSASQPSESQPPSESDWSAGSDKSTAIVPASFSPTG
                     YAALFIHEMPEVRFDDVMVVKVPMEDQEIAIDPRFIEVCHKGTIEVCGIVPEKPILVG
                     ARAVGDKIRLSFAEQDPKHAVRLVLRLTGIRRGFAGKRFPRRTREQFLANEAFINSAY
                     PHDPRAE"
     gene            complement(1552..2352)
                     /locus_tag="PKI05_02275"
     CDS             complement(1552..2352)
                     /locus_tag="PKI05_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51053.1"
                     /translation="MSIRVAERWNSRRWLAGDTPGVELEFIITGTDDDAAAMSAVLGA
                     APGIYANLPRKGRPSLERLGDMAWQATVRYGGLETQDFGLISTEFEIGSQSTKIMQSL
                     ATVGKYAPAGKTPPDYKGAINVSADNVEGVEIEIPTYEWTETWRHPDGTVGPAYGAIL
                     YAVTGRVNNAAFRGFAAGEVLFRGATGRKTREDAWEITYRFAASPNATNLTVGQITGI
                     KKKGWEYLWVVYEDKEDVGAKKLVKHPAAVYVEQVYPTANFSVLGIGS"
     gene            complement(2369..4714)
                     /locus_tag="PKI05_02280"
     CDS             complement(2369..4714)
                     /locus_tag="PKI05_02280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HOA51054.1"
                     /translation="MATPGSIRAGKAHVEIGAVIAPLEAALRKASMTLKAWGSQVQSM
                     GQRAMLAAGAMAAPVALSGRVYAGFSDQMKAVQAVSGATAAEMRMLTNTAEDLGRTTS
                     YTAAQVGEAMLNLARSGFRPKEINDAISGILALARATGTDLALAGDIAAGTLRAFNLE
                     ATESQRVADVLTAAANNSAQTLEDLGEAMKYVAPVAQEYGLSIEETAKAIGALANVQI
                     KGSMAGTSLRQMMVALANPAIRAKIEALGVAVTDATGGFRKDFGKMLLELGEAMKSLP
                     APERLALLYEVFGQRSMAAAAKLAVSNFQGLNDAIDNAAGNAQRAAEIMDSGLGGAWR
                     RLTSALEGVQIAIGKVVELAISPLMDALGNVLVQVAQWINANRAAVMVVLGAVAAVGT
                     LGATLFAAGIGLKAVGVALGTLATIGKLVALAFAGMQAAVTALLSPLGLLVASLAGIG
                     ALAVYVSGAWKPLFAYLGQGFQWIAELAGKTWKGISDALASGNLALAGEIAMAGLKTA
                     WYEAMLWIQGRWIAFKAKIVDIWYGAVYGIAQMALTGFAALQTLWTNVTATFRRLWIR
                     TTTFISDTWDRVYTWLAKSFVNLTKLFSDAHEKAAAKQAIDDEYAARAKDREKAAQEA
                     LTQIEREGAERRQQIDRELMDSLITLEEDRQRAVEQAGSVSEEDKKRLAELKAKADAA
                     RKRLDELTEQAELARAEAEKPPEMPELPEAPQLEEKLAAAGAKPTGLGGGGTAGTFSA
                     LAVRGLAVGGPLERIAKSSEETAENTRRMNKLLEQDEAKFV"
     gene            complement(4698..4976)
                     /locus_tag="PKI05_02285"
     CDS             complement(4698..4976)
                     /locus_tag="PKI05_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51055.1"
                     /translation="MAGIIGIDPGPFTLRQLVWMAEGRRDFFWDQTAHVLCYLYNAHR
                     SPKSPALMPADFHPFADKRKRRMKKRLPQVGIEALKVFLPRGNHGNTG"
     gene            complement(5000..5446)
                     /locus_tag="PKI05_02290"
     CDS             complement(5000..5446)
                     /locus_tag="PKI05_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51056.1"
                     /translation="MKTFRDAIGREWQITLNIYTLTRIRDGVGLNLARPGQDLAGVIG
                     DDLTFAAILWEMVRDQAKQLGLLQEQFLASLTGEVLEQAKSLFVEELVSFFPKLRPVM
                     ETMRQIEETYSQALVAEMERLKAEAQDPEKIREQIRRQIEQEMKAG"
     gene            complement(5465..5926)
                     /locus_tag="PKI05_02295"
     CDS             complement(5465..5926)
                     /locus_tag="PKI05_02295"
                     /inference="COORDINATES: protein motif:HMM:NF017961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HOA51057.1"
                     /translation="MSIASVVLGLNAKLYRNTGTQQSPTWTEIANVKDVTLNLEKGEA
                     DATTRAAAGWRQTVPTLKEATVEFEMVWDPSDEGFSAVKDAFFNNTALDLAIMDGTLP
                     PSTGQTVQGLRAQWAVTKFSRNEPLEEVLTVSVTLKPTYGSVPEWMIETGT"
     gene            complement(5940..>6205)
                     /locus_tag="PKI05_02300"
     CDS             complement(5940..>6205)
                     /locus_tag="PKI05_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51058.1"
                     /translation="FDPASKTVVVGPALLPGRRRNVTVPQVHEHGGRIRTVTREIRWE
                     KGRRRKVIKRKSTVKYPQRPYMAPALEKVRPQLPQFWKDVLNR"
BASE COUNT         1086 a         1944 c         1862 g         1313 t
ORIGIN      
        1 cgagcacggc ggcgatggcc cgccaggtga tgaatcgctg cgtgggcacg ctgtatctcg
       61 gctcattcaa gctggctgcc acctcgtcgt cggtcatcgc ggcgtaaccg cgaccaagtg
      121 ggtcattgcg gagttctgca ataagcacat cgtagaaatc ggacatattc gcctcctatc
      181 attcagccct cgggtcatgc ggataggcgg agttgataaa cgcctcgttg gccaggaact
      241 gttcgcgtgt acgtctcggg aacctcttcc cggcgaagcc ccggcggatg ccagtcagac
      301 gcaagaccag ccgcacggcg tgcttggggt cctgctcggc gaaactgagg cggattttat
      361 cccccactgc ccgggcgccg acaaggattg gtttctcggg cacaatccca cacacttcga
      421 tagtcccctt gtggcagact tcaataaacc gcgggtcgat ggcgatctct tggtcctcca
      481 tcgggacctt gaccaccatc acgtcgtcga aacgaacctc cggcatctca tggataaaca
      541 gggccgcgta gccggtgggc gaaaagctgg ccggaacgat ggccgttgat ttatcgctcc
      601 ccgcgctcca gtcgctttcg ctcggcggtt gggactcact aggttgggac gcactcgatt
      661 ccgacggctg gctttggctg ctttccgacg ggccggagct ctccgatggc gctgatgact
      721 cggagggtat cgaactttcc gacgggccac tgtcgctttg atcgctgtcg ctttggtcgc
      781 tgtcgctttg actcggcggc tcgcagcatc cgaagccgta actgatgact tcaaacttgc
      841 ccgcgtcggg catgaggaca gcccacacga aggccccggc gggaagtcca ctcgggctgg
      901 tgactccaag actgtctcgc acggtgacgg tgatttggcc gtcgcaaggc ggttcatcag
      961 ccgttccagt gagcaagagg gcgtcggcct ccccacagtg cgtcaaaggg gccaagagtc
     1021 gcagccgtcg cacatccatc gggtgattgc ccactcgcac aaagcagcgg cgaaggccgg
     1081 tccccgtctc ccgccagaga atcgccacgg gaccgctcgg tgccgatgcg aatcccgtgc
     1141 aatcgccagc gatgggaaca gcgaagccat gcgactcatt gcctgagaca taggcatggg
     1201 tcaacccggc ggcgacggcc cagccgatgg ccccggcagc gatcggttcc tggacaatcg
     1261 caatccggtt atgctctccg tcgggtatcg ctgcggagaa acatggattc gccagaaact
     1321 cagacaggtt atcagtcggc gaaacgatgg gggcagacac tcggacaacc tgataccggc
     1381 ccaggtcggc ccccgtgtcg ttcctaatct tgatccgggt gtcgatagtt cccgtcttgc
     1441 ccagttgcgc caggaagtta tcgcgttgtt ccaagagttc caacagcgcg ttccaggccc
     1501 cggcggggat tttcagggga tcaccgggtt tcttcttctg ccactgtgac atcagcttcc
     1561 gattcctaaa acagaaaaat tggcggttgg atacacctgt tccacataga ccgcggcggg
     1621 atgtttcacg agtttcttgg cacccacgtc ttctttgtct tcatagacca cccagagata
     1681 ctcccaacct tttttcttga tcccggtgat ctgcccgacg gtcaaattgg tggcattcgg
     1741 cgatgcggcg aagcgatagg tgatctccca ggcatcttcg cgggttttgc ggccggtcgc
     1801 tccacggaag aggacctccc cggcggcgaa gccgcggaag gcggcgttgt tcacccggcc
     1861 tgtcacggcg tagaggattg ctccgtatgc cggcccaact gttccgtcgg ggtgtcgcca
     1921 tgtctccgtc cattcatagg tggggatttc gatttcgacg ccttccacgt tatccgccga
     1981 gacattgatc gctcccttat agtcgggcgg cgttttacct gccggggcat atttcccaac
     2041 cgtagccagc gattgcatga tcttggtgct ttgcgagccg atctcaaact cggtggaaat
     2101 gagcccgaaa tcttgcgttt ccaggccgcc atagcggacg gtggcctgcc aggccatatc
     2161 tcccagtcgt tccagcgacg gccttccctt gcgggggagg ttggcgtaaa tgccgggggc
     2221 cgcccccagc accgccgaca tggcggcggc atcgtcgtcg gtcccggtga tgataaactc
     2281 cagctccaca ccaggtgtgt cgcctgccag ccagcgtcgc gagttccagc gttcggcaac
     2341 ccgaatactc atcgtttttc cctgttaatc atacgaactt cgcctcgtcc tgttccagga
     2401 gtttgttcat gcgacgggta ttctcggcgg tttcctcgga ggatttcgcg atccgttcta
     2461 aggggccacc gacggccaag cctcgcacgg cgagggccga gaaggtcccc gcggtgcctc
     2521 caccaccaag gccagtcggt ttcgcaccgg ctgccgcgag tttctcttcc agttgcgggg
     2581 cctcgggaag ctccggcatc tccggtggtt tttcggcttc ggcccgagcg agttcggctt
     2641 gctccgtgag ttcgtcgagt cgtttgcgtg cggcgtcggc cttggccttc agctcggcaa
     2701 gtcttttctt gtcttcttct gacacggagc ccgcttgctc caccgcccgc tgtcggtctt
     2761 cttccagcgt gatgagcgaa tccatgagtt cccggtcgat ctgctgccgt cgctcggcgc
     2821 cttcgcgctc gatctgcgtg agggcctcct gagcggcctt ctcccggtcc ttggcgcggg
     2881 cggcgtattc gtcatcaatc gcttgtttcg cggcggcctt ttcatgggca tcggaaaaga
     2941 gcttcgtcag gttcacgaag gacttggcca gccacgtata gacgcggtcc caggtgtcgc
     3001 tgataaaggt tgtggttcta atccacagcc ggcggaaggt ggcggtaaca ttcgtccaga
     3061 gggtctggag tgcggcgaag cccgtgaggg ccatctgcgc aatgccatag acagcgccgt
     3121 accagatgtc cacgatcttg gccttgaagg cgatccaccg tccctgaatc cagagcatgg
     3181 cttcatacca ggccgttttc agtccggcca tggcgatctc cccggctagg gccagattgc
     3241 cactggctaa ggcgtcggag attcctttcc aagttttgcc agcgagttcg gcaatccatt
     3301 ggaatccttg cccgagatag gcgaagaggg gcttccaggc cccgctgaca tagacggcca
     3361 aggccccgat cccggcgaga ctggccacca gcagccccag cggtgacagc agggcggtca
     3421 ccgcagcctg catcccggcg aaggcgagtg ccaccagctt cccgatggtg gccagcgtcc
     3481 ccaaggccac cccgacggcc ttcaagccga ttccggcggc gaagagggtg gcccccagtg
     3541 tccccaccgc agccaccgca cccaggacca ccatcacggc ggcccggttg gcgttgatcc
     3601 attgtgccac ctggaccagg acgttgccca gcgcgtccat gaggggtgaa atggctagct
     3661 caaccacttt gccgatggcg atctgcacac cttccaaggc cgacgtgagc ctccgccagg
     3721 ccccacccag gccggagtcc atgatctcgg cggcccgttg ggcattgcct gccgcgttgt
     3781 cgatggcgtc gttgaggcct tggaagttgg agacggcgag tttggcggcg gcggccatcg
     3841 aacgctggcc aaagacttca tacaagaggg ccagtctctc cggggcgggg agcgacttca
     3901 tcgcctcccc cagctccaaa agcatcttgc caaaatcttt gcggaatcct cccgtggcat
     3961 cggttacagc cacgcccaag gcctcgatct tggcccgaat cgccgggttg gccagagcca
     4021 ccatcatttg ccttagcgag gttccggcca ttgagccttt gatctggacg ttggccaggg
     4081 ccccgatggc cttggcggtc tcttcgatgc tcaagccata ctcctgggcc acgggggcca
     4141 cgtatttcat ggcctcgccc aggtcctcca gggtttgggc ggaattgttg gccgcggcgg
     4201 tcaggacgtc ggccacccgc tgggactcgg tggcctccag gttgaaggcc cggagcgtcc
     4261 cggcggcgat gtcgccggcc aaagccaggt ccgtcccggt cgcccttgct agggcgagaa
     4321 tgccgctgat ggcatcgttg atttctttgg gtcggaagcc cgagcgggcg agattaagca
     4381 tcgcctcccc gacctgggcg gcggtgtagc ttgtcgttcg gcctaggtct tcggcggtat
     4441 tagtcagcat ccgcatctcg gcggcggtgg ccccggagac ggcctggacg gccttcatct
     4501 ggtccgaaaa gcccgcatag acccggccag agagggccac gggggcggcc atggccccgg
     4561 cggccagcat cgcccgttgc cccatcgact ggacctgact cccccaggcc ttgagggtca
     4621 tcgaggcctt gcggagagcg gcctccagcg gggcaatcac cgccccgatc tcgacatggg
     4681 ccttcccggc acgaatacta cccggtgttg ccatgattcc ctctcggcaa aaagaccttt
     4741 aaggcctcga ttccaacctg cgggagacgt tttttcatac ggcgtttccg cttatctgcg
     4801 aatgggtgaa agtccgctgg catgagggcc ggcgatttcg ggctgcgatg ggcgttatac
     4861 aggtaacaca agacatgggc cgtctgatcc cagaaaaagt cgcggcggcc ctcggccatc
     4921 cagaccaatt gccgcagcgt gaacggtccc gggtcgatcc cgatgatccc ggccaaacgg
     4981 taaatctcag cccaaatatc tatcccgcct tcatctcctg ctcgatctgc cggcgaatct
     5041 gttcgcgaat cttctccggg tcctgagcct cggccttgag tcgttccatc tccgctacca
     5101 gggcctgact gtaggtctcc tcgatctgcc gcatcgtctc catgacgggg cggagcttcg
     5161 ggaaaaaact gaccagttcc tccacaaaga gcgatttggc ctgttccagg acctccccgg
     5221 tgagactggc caggaattgc tcttgcaaca aaccgagttg tttggcctga tcgcgaacca
     5281 tctcccagag gatcgcggcg aaggtcaaat catcgccaat caccccggcc aggtcttgcc
     5341 cgggtcgggc caggttgagg cccaccccat cgcggatgcg agtcagggtg tagatattga
     5401 gggtgatctg ccactctcgc ccaatggcgt cacggaacgt tttcatgagt ttgatttctc
     5461 cttattaagt cccggtttca atcatccatt cgggcaccga gccgtaggtc ggtttgagtg
     5521 tcacggagac cgtgagcacc tcttccagtg gttcgttccg actgaatttg gtcacggccc
     5581 attgggcccg caggccttgg acggtttgac cggtggaggg cggcagcgtc ccatccatga
     5641 tggccaaatc cagggccgta ttattgaaga acgcatcttt gacggcagaa aagccttcgt
     5701 cgctggggtc ccacaccatc tcgaactcga cggtcgcctc tttcagagtg gggacggttt
     5761 gccgccagcc agcggcggcc cgggtcgtgg cgtcagcctc ccccttttcc aagttgagtg
     5821 ttacgtcctt gacgttcgcg atctcggtcc aggtggggct ttgctgggtt cccgtgtttc
     5881 tgtagagctt ggcgttcagc ccgaggacaa cggaagcaat ggacatggtg atcgtctcct
     5941 tatcggttca agacatcctt ccagaattgg ggcagttggg ggcggacctt ctccagggcc
     6001 ggtgccatat agggtcgctg cggatatttc acagtggatt tgcgtttaat gaccttgcga
     6061 cggcggcctt tctcccagcg gatttcgcga gtcacagttc tgattctgcc accatgctcg
     6121 tgaacttggg gcaccgtcac attgcggcga cgccccggta acaaggccgg cccaacaacc
     6181 acggtcttac tagccgggtc aaaga
//
LOCUS       DAOPTR010000069         6200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_10365_length_6200_cov_3.468674, whole genome shotgun sequence.
ACCESSION   DAOPTR010000069 DAOPTR010000000
VERSION     DAOPTR010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10365_length_6200_cov_3.468674"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            322..963
                     /gene="rsmD"
                     /locus_tag="PKI05_02305"
     CDS             322..963
                     /gene="rsmD"
                     /locus_tag="PKI05_02305"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOA51059.1"
                     /translation="MARRRGKAEDEGQPKRRTPVGLRIIGGRLRGRRLLYDGSWSIRP
                     MRDRVREAIFNILRDAVVDAHVIDIFAGTGAMGLEALSRGAKFVQFIERDPTACRVLL
                     QNIERLQLQEQTEVIVSDAFIWWKHCGPLENVPHVVFCCPPYELYQTRGHEMLQLITG
                     LVRSLPNDSVLVVESGEDVDVHQFPYPDKWDVRLYRPSRIGFFWKTSSSDEAA"
     gene            complement(1005..1451)
                     /locus_tag="PKI05_02310"
     CDS             complement(1005..1451)
                     /locus_tag="PKI05_02310"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOA51060.1"
                     /translation="MADRLKILFLCTGNSCRSQMAEGWARHLKGDQIEAHSAGIQPQG
                     LNPRAVAVMAEAGVDISGHTSKPLAEVMSIPFDYVITVCDDAHEACPVFPGKAQVVHV
                     GFPDPPRLTRHLQDEEAILQVYRQVRDAIRDFVQGLPDNLCNLARK"
     gene            complement(1540..2547)
                     /locus_tag="PKI05_02315"
     CDS             complement(1540..2547)
                     /locus_tag="PKI05_02315"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOA51061.1"
                     /translation="MSDFYPMGRDPVQRPPQWPYDGGSFQAEHVGYEFDSERDQPMRP
                     STAPPEVVMAELAGPPPRSRARFVVPLVLLILTIISTFVAAGGLGNPWDGLKYSVCVM
                     AILLSHEMGHFLQALRYRAPATLPFFLPMPFSPVGTFGAVIAMDTRRLDRRALFDIGA
                     TGPLAGLVPTIVFTVVGLQQSQVVSAARIPGVMELGEPLLFTWLTRLIHGPLPEGYTV
                     MLGPMAFAGWVGLLITALNLIPIGQLDGGHILYALLGRKANTVGMFLLLGAIVAVVIA
                     GYWGWLIMIFLLGLLGPEHPPTANDRAPLGALRIILGWLMLAFIIVGFTPEPFKLFPQ
                     S"
     gene            complement(2902..4446)
                     /locus_tag="PKI05_02320"
     CDS             complement(2902..4446)
                     /locus_tag="PKI05_02320"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOA51062.1"
                     /translation="MLVWVVFITVATINLILGIAVAVWMKRGIAGHQSDDLSNNLELF
                     SGIIPSPPSTTVSQQSTNSDDEAADRDAPPNDSISPDITASDSATYAESAAANSTTEA
                     FVVDRLGSTQESDSSESPVNVNSAEIASDKTRMPESEDQGPVEPASEESPPQSGPQED
                     DVPSETDAEAAVEAPSSEEGEIPGLSRLLELCSKKVHELIDIFEWMRSQPPTKGEELN
                     TILARVPPITASFFDEYGQIVPKLVSTEHGGPIPEETLACLEEHALAAEQTVQRLTML
                     DYRGHLQATLQQALAEFGRLIARLQRTRDELEAAFAQGLINFQPHKEVDEKIQRDALT
                     GLFNRTGVEVFFEKHWGGEPHKRKPLWAAILDLDQFRQINQEHGYSLGDELLKAVAQL
                     LAEVVPQGAFLARYRGDQFLVVRTDCQSKRFIHTIEEVRQSVEVTKFRYAGQTFSLTV
                     SCGVTESRTDDVLTSFCERLDEALLEAKRAGGNRTFIHNGEFARPIIPLNLKVAEREV
                     SVATVG"
     gene            complement(4577..>6200)
                     /locus_tag="PKI05_02325"
     CDS             complement(4577..>6200)
                     /locus_tag="PKI05_02325"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOA51063.1"
                     /translation="ITPRPLRVYREEVPIPPDCDLATLPGWRALCRSFLQTTGCLLRF
                     LPGAQPSSPDDIPVMPPTVDASGPVGVRSPLGHLRIDPTTRAPKGLNPDHPIYRMARG
                     MAEVLGELLATRIALWQREAELAAGIPVIPHKEEEQHLAQRLDEILHSATRAVDAQAA
                     AIYILDETTSHLKLRAIWGLPKTKLLAPPRNLKSAMADLEAMLGHAVIVDRSQPVDHW
                     NPPEEFATSVCLPISTPTTILGTLWVFSNQQRTFSDRDIDLLEVAAGRVAAELEREAA
                     LQAALQTAQLQRQVERAQRLIRSQLPSVSPLVDGWELAGWMDTERMGGAFFDWCCPKD
                     GRIHFALGEAAEDSVSTALTMAAVKAAWRAHAQYQRETKKLLEQLNLTLWTSSAGDQS
                     AIMVCGIVDPKTGTLWLAAAGTAYAMLITEKQHRVLPPGGANLGVDPESTYLPVRWKF
                     PENAILVATTAGVRSAVGVDQRPLWETQILPLLTSNLHLPAKILVQHVQRAIEQNAVT
                     PNKVPAVLIIKRRPSPKHPETDSRLRENSQHA"
BASE COUNT         1333 a         1672 c         1871 g         1324 t
ORIGIN      
        1 cgcgttcggc aggacttacg aaaattgaac cccaacgcac ctgtgttttg cacctcggcc
       61 aagacgggag agggcatcga ggaaatcgcc acgtggctgg ctaacgcagc gacggggcga
      121 taggccgcgg ttcttgcgat cggacgggac attaggccac gtgttgtggg atgatctcgc
      181 gcgatatcgg aagaaaatct gttctcctcc cgcgctattg ctgcgcttgg gagaaatgga
      241 aagcgatgtt acaatgcaac gtggtccgag ccgtggttca taagaccttt tccaggcacg
      301 ggatatgatt gatagctgaa catggccagg cgtcgaggaa aagcggaaga cgaaggtcag
      361 cccaagcggc ggacaccggt tggcttgcgg atcatcggtg gccgacttcg cggaaggcgt
      421 cttctctacg atggttcctg gtcgattcgc ccaatgcgcg atcgggtgag ggaggcaatt
      481 ttcaacatct tgcgggatgc cgtggtcgat gcccacgtca tcgacatctt tgccgggacc
      541 ggggccatgg gattggaggc cctgagtcgc ggggccaaat tcgtgcagtt tatcgagcgc
      601 gatccaacgg cgtgccgcgt gctgctgcag aacatcgaac ggcttcagtt acaggagcag
      661 acagaagtta ttgtcagcga tgccttcatt tggtggaaac actgcggccc attggagaat
      721 gtgcctcatg tcgttttctg ttgtccaccg tacgagctat atcagactcg tgggcacgaa
      781 atgcttcagc tcataacggg gctagttcgg tccctcccca acgatagcgt tctcgttgtg
      841 gaatctggtg aagatgttga tgttcaccag tttccttatc ctgacaaatg ggatgtgcgg
      901 ctctatcgcc ctagccggat tggctttttt tggaagactt catcgagcga cgaagccgcc
      961 tgatcgtcgc gtcgaagacc gaacgatgtt gcaccgcccc cgtgctattt ccgtgctagg
     1021 ttgcaaagat tgtccggcag tccctgaaca aaatcgcgga tggcatcgcg aacctgacga
     1081 tagacttgga ggatcgcttc ctcatcttgc agatgccggg ttaaacgggg tggatcagga
     1141 aagcccacat gaaccacctg agccttgcca ggaaagacgg ggcaggcctc gtgggcgtca
     1201 tcgcacacag tgatcacgta atcgaagggg atggacatga cctcggcaag gggcttacta
     1261 gtgtggccgg agatgtcaac cccggcttct gccatcacgg ccactgcccg tggattaagc
     1321 ccttggggct ggatacccgc cgaatgcgcc tcgatttgat cacctttaag gtggcgtgcc
     1381 cagccttcgg ccatctggct tcggcacgag tttccggtgc atagaaagag gatctttagc
     1441 ctatcggcca tggctagtgc ttttcctgct tacagtgacc gatcttccca acgggccttt
     1501 cttttcactc atcgatggcg atcgttcatc aagacaattt tatgactgag ggaagagttt
     1561 gaacggctca ggggtgaatc ccacgatgat gaaggcgagc atcagccagc ccaagatgat
     1621 ccggagcgcc ccgagtggtg cacgatcgtt tgcggtcggg ggatgttccg ggccgagcag
     1681 tcccaacaag aatatcatga tcaaccaacc ccaataacca gcgataacca ctgctacgat
     1741 cgccccgagc aataaaaaca ttcccacggt gttggccttt cgaccaagta gggcgtaaag
     1801 gatatggcca ccatccaact gaccaatggg aataaggttg agcgccgtaa taagcaagcc
     1861 gacccaaccg gcaaaggcca tcggcccaag catgacggta taaccctcgg gcagtggtcc
     1921 gtgaataaga cgagttaacc aagtgaaaag gagcggctcg cccaactcca tgacaccggg
     1981 aatcctggca gccgagacga cctgggactg ttggagaccg acgacggtga agacaattgt
     2041 gggaaccagg ccagctaatg gaccggtggc gccaatgtca aaaagggctc ggcggtcgag
     2101 acggcgtgta tccatggcga tcacggcccc gaacgttccc acgggagaaa atggcatcgg
     2161 gaggaaaaag ggtaatgtgg ccggcgcacg gtagcggagc gcttgcaaaa aatgacccat
     2221 ttcgtgggat aagaggatgg ccataacgca aaccgagtat ttcaggccgt cccacgggtt
     2281 tcctaacccg ccggctgcca cgaacgtgct aatgatcgtc aggatcaaca aaacaagcgg
     2341 gacaacaaag cgtgctcttg aacgaggcgg tggcccggca agctcggcca tcaccacttc
     2401 cggtggggcg gtgctgggcc gcatcggctg atcccgttcg gaatcgaatt catagccgac
     2461 atgctctgcc tggaacgatc cgccgtcgta gggccattga ggtggtcgtt ggacggggtc
     2521 acgccccatc ggataaaaat cgctcatacc tatgcccacc aatcaagtgt aaggaattga
     2581 gtttacaaga ctgtgcaagt ctcacacaca ggagcccatc ccaaaaagtc cccctctccc
     2641 ggagggagag ggttggagtg agggcaagcg aacgtcggaa tcgattgaaa aaaacagcag
     2701 cgattcgagc cacgctcacc cccgacccct ctcccgccga atgggcaagg gaagattacg
     2761 ggataggctt tagctgccca tcttatccta agtgtacagg atttctaggt tttgagtatg
     2821 gcttctacga agacagttac cgcaagaaag ccctgtggtc gtctaaagac gtttcaaacg
     2881 catgtttgag agcgcctgga attatccgac tgtggcgacg gacacttccc gttctgcgac
     2941 tttgagattg agcggtatga tcggtcgggc gaactcgccg ttgtggatga aggttcggtt
     3001 accccccgcc cgtttggcct ctagcagggc ttcgtccaat cgctcgcaaa aggaggtaag
     3061 gacatcatct gttcgagatt ctgtcactcc gcagcttacg gtgagggaga atgtctggcc
     3121 agcgtagcgg aacttggtga cctccaccga ttgacgcact tcctcaatgg tatggatgaa
     3181 gcgtttcgac tggcaatcgg ttcggacaac gaggaactga tcgccgcggt atcgtgccaa
     3241 aaaggccccc tgggggacaa cctcggcaag aagctgggcg acggctttta ggagctcgtc
     3301 accgagtgag tacccatgtt cctggttgat ttgccgaaat tgatcgaggt cgagaatcgc
     3361 agcccacagt ggcttgcgtt tgtggggctc acctccccag tgtttttcga agaagacttc
     3421 aacccctgtc cgattgaaca atccggtcag ggcatcgcgt tgaatctttt catcgacctc
     3481 cttatgcggc tgaaagttga taagcccctg ggcgaaggcc gcttccagtt cgtcgcgcgt
     3541 ccgctggagc cgcgcgatca accgcccgaa ttcggcaagg gcttgctgga gcgtggcttg
     3601 gaggtgacca cgataatcga gcatggtcag ccgttgtacg gtttgctcgg cggcgagggc
     3661 atgctcctct agacaggcta aagtttcctc ggggattggc cctccgtgtt cggttgagac
     3721 tagcttgggc acgatctggc catactcatc aaaaaatgat gccgtgatgg gaggcacccg
     3781 ggccaggatt gtgttaagct cctcgccttt ggttgggggt tgcgaacgca tccactcgaa
     3841 gatatcgatc aactcgtgga ccttcttgga gcataactca agaaggcgac tcaggcctgg
     3901 gatttcgccc tcttccgagg agggggcctc gacggcggct tccgcgtctg tctcgctcgg
     3961 aacgtcgtct tcctgcgggc cggactgcgg tgggctttcc tcggatgccg gctcgacagg
     4021 gccctgatct tcggattcgg gcatcctagt tttgtcgctg gctatctcgg cagaattgac
     4081 gtttaccggg gactcggagg aatcggattc ttgagtacta cccaaacgat caaccacaaa
     4141 agcctccgtg gtgctattcg ccgccgcaga ctccgcatag gtggcagaat ccgacgctgt
     4201 tatgtcaggt gaaatagagt cgttaggtgg ggcgtcccgg tccgcggctt catcgtcgga
     4261 attggtggat tgctgggaca ccgtagtgct cggcggtgag ggtatgatgc ctgagaatag
     4321 ctccagattg ttggaaaggt catcactctg gtgcccggcg atccctcgct tcatccatac
     4381 agcgaccgcg atccccagga tcagattgat tgtggcgacg gtgataaaga ccacccacac
     4441 caacatgagg cgctgccatt ttcaatattc aataatcctc gggaatctcc attaccaaca
     4501 agtcggaaaa gccctttcta tgggccacaa gtcggaaaag cccccgacaa gggctatatg
     4561 tcggcagaaa cgttaatcaa gcatgttgag aattctccct gaggcgtgaa tccgtttcgg
     4621 ggtgttttgg gctaggtcgc ctcttgataa tgaggacggc cggcaccttg tttggtgtaa
     4681 cagcgttctg ctcgatcgcc cgttgcacgt gctgtaccag gatcttggcc ggtaggtgaa
     4741 gattcgatgt cagcaagggg aggatttggg tctcccacaa tggacgctgg tccaccccaa
     4801 cagcggatcg gactcctgcg gtcgttgcca cgaggatggc gttctcgggg aatttccaac
     4861 gcacaggtag ataggttgat tcgggatcaa cgcccaaatt ggcgcccccg ggtggcagga
     4921 cgcgatgttg tttttcggtg attaacatgg cgtaggcggt ccctgctgcg gctagccata
     4981 acgtacctgt cttcggatca acgatgccgc agaccatgat ggccgactga tcaccggcgg
     5041 aactggtcca gagcgtcaaa ttgagttgtt ccaggagttt cttggtttcc cgctgatatt
     5101 gggcgtgtgc ccgccaagcg gcctttaccg ctgccatggt cagggccgtg gagaccgaat
     5161 cttctgcagc ctcgccaagg gcaaaatgga tgcggccgtc tttgggacaa caccagtcga
     5221 aaaaggcgcc gcccattcgc tcggtatcca tccacccggc cagctcccag ccgtccacga
     5281 ggggtgatac actgggaagc tggctgcgaa taagccgctg cgcacgttcg acttgtcttt
     5341 ggagttgtgc cgtttgcaag gcggcttgga gggcggcctc gcgttccagt tcggcagcga
     5401 cccgacctgc tgcgacttcc aggaggtcga tgtcgcggtc cgagaacgtc cgctgctgat
     5461 tgctgaacac ccaaagtgtg ccaaggatcg ttgtgggcgt ggaaatgggc aaacagacgg
     5521 aagtcgcgaa ctcttccggg gggttccagt gatcaactgg ttgcgagcgg tcaacaatga
     5581 ccgcgtgacc gagcatggcc tccaggtctg ccatagccga ctttaagttc cgcggaggtg
     5641 ccagcagctt ggtcttcggc aaaccccaga tggcccgcag cttgagatga ctggtcgttt
     5701 catccaggat gtagatggcc gcggcctgag catcgactgc gcgcgtggcc gagtggagga
     5761 tttcgtccaa ccgttgtgcg aggtgctgtt cttcctcttt atgggggatg actggaatcc
     5821 cggctgccag ttccgcctct cgttgccaaa gggcgattcg ggttgccagc aattcgccaa
     5881 gcacctcggc cataccgcgg gccatgcggt aaatggggtg atccgggttg agtcccttgg
     5941 gggcccttgt cgtaggatcg atccgcagat gacccaaggg cgaccgtacc ccaaccgggc
     6001 cagaagcgtc aacagttggg ggcatcacgg ggatgtcgtc cgggctgctc ggttgagccc
     6061 ccggtaaaaa gcggaggagg cagccggtcg tctggagaaa tgaccggcag agcgcccgcc
     6121 aacccgggag tgtggcgagg tcacaatcag gtgggatcgg gacctcttct cggtaaactc
     6181 gtaaaggcct cggcgtgatc
//
LOCUS       DAOPTR010000070         6183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_10396_length_6183_cov_3.267787, whole genome shotgun sequence.
ACCESSION   DAOPTR010000070 DAOPTR010000000
VERSION     DAOPTR010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10396_length_6183_cov_3.267787"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /gene="lipA"
                     /locus_tag="PKI05_02330"
     CDS             <1..811
                     /gene="lipA"
                     /locus_tag="PKI05_02330"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOA51064.1"
                     /translation="TARLIESLGLATVCEHARCPNRMECYARKTATFMILGEVCTRRC
                     GFCAVKKGKTQPVDASEPQRVAEAVKALGLRHVVITCVTRDDLPDGGAEHFAQTIAAV
                     RAVSGATIEVLPSDFAQRMESVDRVCAARPDVYNHNSETVPRLYRTVRGRKANYRWTL
                     EMFRHIKAEFPGICTKTGLMLGLGETYQELLDTFADFVAAGVDILTLGQYLRPSSDCL
                     PVVRYVPPEEFEELAQRAKQIGFKQVFAGPFVRSSYHAGEVFGAVRSTSSS"
     gene            856..2043
                     /locus_tag="PKI05_02335"
     CDS             856..2043
                     /locus_tag="PKI05_02335"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOA51065.1"
                     /translation="MSRTIFISAGEPSGDQHGAALLKALRRRDAGISAIGFGGPKMME
                     AGLQALADLTAHAVMWFSRPLAQLRQFWRFYRMAVESLREHRPQVVILIDFPGFHWHL
                     AKAAKKLGITVFYYGLPQLWAWAPWRVKKLRRWVDHTFAWLPFEAPWLEHHGCRVTYV
                     GHPFFDEAASHTFDSTFVARFQGSRPLLVLLPGSRDQEVSASASLMLRTVQRLREAIP
                     SLHVAVAAFRPEQAKRVQNLAEDIQLPLEVFAGKTPELIRAADCCLAVSGSVSLELLY
                     HEKPTVIVYRIGRLAYHVQSWFRRVKYITLVNLLKYGGEQAAYLSPRDHNTPDPENAL
                     FPEFLTYRDCSRQMADCLQRWLIDTAAQEEVIKELRQLKTSLAWEGAVDRAAEAIHGF
                     LVQ"
     gene            2231..2959
                     /locus_tag="PKI05_02340"
     CDS             2231..2959
                     /locus_tag="PKI05_02340"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOA51066.1"
                     /translation="MSSFDPHETPYDLYFTLFGIPVRVHPLFWLVAVFLGLHADDLFS
                     LLAWVLAVFIAVLVHEMGHAVAMRAFGYYPSVTLYGMGGYTRYGMRAVGVLPPRVWGR
                     ILMTAAGPAAGFLLALVLALGLSVLRVPLVIKLGPPIGVMVLANLPSPWLTVFVYYIF
                     FTTIAWGVLNLLPVLPLDGGHIMREILSIRDPHWGSFRAGQISLVVAAAVAFWSVMSG
                     SYFLAILFAYLAYSNYQALAGPRF"
     gene            complement(3037..3819)
                     /locus_tag="PKI05_02345"
     CDS             complement(3037..3819)
                     /locus_tag="PKI05_02345"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOA51067.1"
                     /translation="MSERFFVAQLTTTGEITLSGPEAHHLIHVMRGHVGQQVVLFDGL
                     GHEAVAEITAIQKKTAVLRIAEHGTVSRELPVAVTVASPLPKGDRERFFIEKLTELGV
                     TAFIPLITHRSVVQPDENVVNRLRRIVIEASKQCGRNHLMDVTAPCKWEKLIAEPQEG
                     VTRLFAHPFSVERDGPPQPLGSDRTSGLPASLEALLTSEKVLVAVGPEGGLTPEEVGQ
                     ALAKRWIQVDLGPRILRVETAAIVLAVLVGTIRQVRAQTQGS"
     gene            4004..4876
                     /locus_tag="PKI05_02350"
     CDS             4004..4876
                     /locus_tag="PKI05_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOA51068.1"
                     /translation="MTREISAVANPVVTRCLEYWGIRSQPFSPQRSAETFFLSSGAEE
                     VLVRLDYVVREGYAGAVLVGSSGVGKTLLLRRALQTWRRHRRAVVFVNATGQDRLSLT
                     TALAYELGASRSEGPAAILWQELRDHVVARLWSYEACVIICDDVARGTPDVLGLLLAL
                     GQITTNGRRPTLIGSCRHDEIHYPTVHQQSWYDIHVELPPWSVEEVQQYLCDRLREAG
                     TDPAEVFTLSAMERLAELTGGNPRDVNRLAEWCLLVAAGAEVRPIGPELVTDVFEELY
                     SQVGDLVLPRSSRG"
     gene            complement(4923..5513)
                     /gene="lptE"
                     /locus_tag="PKI05_02355"
     CDS             complement(4923..5513)
                     /gene="lptE"
                     /locus_tag="PKI05_02355"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HOA51069.1"
                     /translation="MLGVCRRRHRPRQSPSAWILLTWLALVPTSLIILTATLGCAGYR
                     IGNATLFRPDIQTVYVPVFQSTSLRPGLGERLTEAVIKEIELRTPYKVVGSLDRADSI
                     LTGRITSETKRTVVENPYDDPREIATRLQVEVQWIDQRGRVLTPEEAIPVPPELSTVV
                     AETTLVPEVGQSLATAQQAALQRLAMQIVGMMENPW"
     gene            complement(5539..>6183)
                     /gene="bamD"
                     /locus_tag="PKI05_02360"
     CDS             complement(5539..>6183)
                     /gene="bamD"
                     /locus_tag="PKI05_02360"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOA51070.1"
                     /translation="QVELSRPSFLGIVQVGDKTRPTFDTWGRAIKAYQNVRLHDPTGP
                     LADDAIMATANAYFLKQRYEDAAYHYDLLRKDYPQSEHAVNAAFLAIKAHQLTYQGPL
                     YDGTPLERAEQAADEILAVYNSKLGDEREEVIRVKNQINEEKALRYFAIGQYYDNRKY
                     YGAARYYYRATLREFPHTKAAVMAAERIRQIDGLPDKPPNRFKWLTDLFERRDD"
BASE COUNT         1146 a         1676 c         1872 g         1489 t
ORIGIN      
        1 caccgcccgg ctgattgagt ccctcggcct ggctaccgtg tgcgagcatg cccgatgccc
       61 caatcgcatg gaatgttatg cgcggaagac ggccaccttc atgatcctgg gggaggtctg
      121 tacacgccgc tgcggctttt gtgccgtcaa aaaaggaaag acacagcccg tggatgcttc
      181 cgagccccag cgcgtcgcgg aggccgtcaa ggccctgggg ttgcgtcacg tggtgatcac
      241 atgcgtgacg cgggacgatt tacctgatgg tggggcggag cattttgccc agacgatcgc
      301 cgctgtgcgg gcggtcagcg gtgccaccat cgaggtcttg ccttccgatt tcgcacaacg
      361 catggagtcc gtggaccgtg tttgtgcggc ccgaccagac gtttacaatc acaactcgga
      421 aaccgtaccg cggctctatc gtaccgtcag ggggcgcaag gctaactatc gttggacgct
      481 ggagatgttc cgccatatca aggccgaatt tccgggcatc tgcacaaaga cgggtttgat
      541 gctgggattg ggcgaaacct accaggaatt gcttgatact tttgccgatt ttgtggcggc
      601 tggtgtggac attttgacat tgggccaata cttgcggccc tcgtccgatt gcctgcctgt
      661 tgtccgctat gtgcccccgg aggagttcga ggaactggcc cagcgggcga aacagatcgg
      721 gttcaagcag gtctttgccg gaccatttgt gcgatcgagt tatcatgccg gtgaggtgtt
      781 tggagcggtg cggagtacct cttcatcctg atcgggagct atgggaacac ggtgtctcat
      841 cgcagggagg cgtctatgtc acgaacgatc ttcatttcgg ccggcgagcc cagcggtgat
      901 caacacggtg ccgcgctttt gaaggccttg cgacggcggg acgccggcat atcggccatc
      961 ggatttggcg gtcccaagat gatggaggcg gggttacaag ccttggccga tcttacggcc
     1021 cacgccgtga tgtggttttc ccgaccgctt gctcaacttc ggcagttttg gcgattctat
     1081 cgcatggcgg tggaatcgct tcgcgaacat cgcccccagg ttgtcatttt gatcgacttt
     1141 ccggggtttc actggcacct ggcgaaggcg gccaagaaac tcggaatcac ggtgttctat
     1201 tatggtcttc cgcagctttg ggcctgggcg ccgtggcggg tgaaaaagct ccgccgctgg
     1261 gtggatcaca cgtttgcctg gcttcctttt gaggccccct ggttggaaca tcacggttgc
     1321 cgtgtgacct acgtgggaca tccctttttc gacgaggcgg catcccacac attcgactcg
     1381 acgtttgtcg cccgctttca ggggtcgcgt cctttgctcg ttctcttgcc gggttcgcgg
     1441 gatcaggagg tctcggcctc tgcctcgctc atgttgcgga ctgtgcaacg tttgcgagag
     1501 gccatcccga gtttacacgt cgcagtggcg gccttccgac cggagcaggc gaaacgagtc
     1561 caaaaccttg ccgaggacat acaactgccc ttggaggtct ttgctgggaa gacccctgaa
     1621 ttgattcggg cggcggactg ctgtttagcc gtttctggct ccgtctcgct tgaacttctt
     1681 taccacgaaa agcctacggt gattgtatac cgcatcggac gattggctta ccacgttcaa
     1741 tcgtggtttc gtcgtgtgaa gtacattacg ctagttaact tgctcaagta tggaggcgag
     1801 caggctgcct atctttctcc ccgggatcac aatacgcccg atcctgagaa cgccctattc
     1861 ccggagttct tgacctaccg cgattgcagc cggcagatgg ccgattgcct ccagcgatgg
     1921 ttaatcgaca cggcagcgca ggaggaggtg ataaaagaac tgcggcagtt gaaaacatct
     1981 ttggcctggg aaggggcagt ggatcgagcg gccgaagcga tccacggttt tctggtgcaa
     2041 tgatgcagtc ccggggtgtc tggggcaccg tacccttttg acaaagtaga attgtcaaac
     2101 ccacagtatt taacgaaaat gggaaccatt gtactttctg tgagcggatg gggcaaaacg
     2161 gtcatgtggg aaacgggacg attagtggtg ctcataggaa ctggtaaatg acaaggattg
     2221 ggatgaggtt gtgagttcat tcgatcccca cgaaacgccc tatgatcttt atttcacgct
     2281 ctttggtatt cccgtgcggg ttcatccgct gttttggctt gtggccgtgt tcctggggct
     2341 acacgcggac gaccttttct cgcttttggc ttgggttcta gcggttttta tcgcggtgct
     2401 ggttcacgag atgggccatg ccgtggcgat gcgggcattc ggctattatc catcggtcac
     2461 gctttatgga atgggaggat acacgcgata tgggatgcgg gccgtgggtg tgttgccgcc
     2521 tcgggtctgg ggacggatcc tgatgacggc cgccggaccc gccgcgggat ttctcttggc
     2581 ccttgttttg gccctcggtt taagcgtcct gcgggtgccg ctcgtcataa aacttggtcc
     2641 gcctatcggc gtcatggtcc ttgccaattt gccgtcccct tggttaaccg tgtttgtcta
     2701 ttacattttc ttcaccacca ttgcctgggg tgtcttgaat ctgttgcctg ttcttcccct
     2761 cgatggtggc catattatgc gggagatact gagcatccgg gatccgcatt gggggagctt
     2821 tcgggcaggg cagatttcgt tggtggtggc tgccgccgtg gcattctggt ccgtgatgtc
     2881 cggcagttat ttcttagcga ttctttttgc ctatcttgct tattccaact atcaagccct
     2941 cgctggcccg cgattctgat ccaattttga tgggcgtaaa cagcgcccgt tagccggttg
     3001 cgcgaccaat tacggcaaac tactttatag gcctcttcat gacccctgtg tttgggctcg
     3061 cacctgccgg atagtgccaa caaggaccgc caaaacaatg gcggcggttt ccacccgcaa
     3121 aatcctcggg ccaagatcga cctgaatcca cctcttggcg agggcttggc caacttcctc
     3181 gggggttaat cctccttccg gtcccaccgc aacaaggacc ttttccgagg tgagaagagc
     3241 ctcaagactt gccggaagac cacttgttcg gtcgctgccc agtggctggg gcggcccgtc
     3301 tcgctcaacg gaaaaagggt gcgcgaaaag cctcgtgact ccctcttgcg gctctgcgat
     3361 aagcttttcc catttgcatg gggctgttac atccataagg tgattccgcc cacattgttt
     3421 gctggcctcg atcacaatcc gccggaggcg attgacgacg ttctcgtcgg gctggaccac
     3481 actccggtgg gtgatcaggg gtatgaaggc ggtgacgccc agttcggtca atttttcaat
     3541 gaagaatcgt tcacgatcac ctttggggag ggggctggcc accgtaacgg cgacgggcaa
     3601 ttcccgcgat accgtcccat gttccgcaat ccgcaacacg gcggtcttct tttggatagc
     3661 cgtgatctcc gctacggcct cgtgtcccaa accatcaaaa aggactacct gctggccgac
     3721 gtggcccctc atgacgtgga tcaagtgatg ggcctcggga ccgctaagcg taatctcgcc
     3781 ggtggtggta agttgggcaa caaaaaagcg ttctgacatc gtttgacata ggaggcttgg
     3841 ttgcggatga actgcggcaa taccggagta tgccgggccg ctgtcaaact ttatagattt
     3901 tagcagaaag tctgacgatg aagagaataa ggggtattac ggccacgtac tgcgaagggc
     3961 cgaagctcaa agtatcctga catccacccg ttatggtgac aatatgacgc gtgaaatctc
     4021 ggccgtggca aatccggtgg tgacgcgatg cctggagtat tggggcattc gcagtcaacc
     4081 tttcagcccg cagcgttcgg cggagacgtt ctttttatcg agcggggctg aggaggtcct
     4141 ggtccgtctc gactacgtcg taagggaggg gtatgccggg gccgtcttgg tgggttcatc
     4201 tggggtgggc aagacactcc ttctccgccg tgctctccaa acttggcggc ggcaccgccg
     4261 ggccgtggtt ttcgttaatg ccaccggtca agatcggctt tcgcttacca cggcattggc
     4321 ctatgagttg ggggccagcc gcagcgaagg accggccgcc atcctttggc aggaactgcg
     4381 ggatcacgtg gtggctcggc tttggtcgta tgaggcctgc gtgatcatct gtgacgatgt
     4441 ggccagaggc actcccgatg tcctcggcct ccttttggcc ttgggacaga ttacaacgaa
     4501 tggccgtcgg ccaaccttga tcggctcttg ccggcatgat gagattcatt atcccaccgt
     4561 gcaccaacaa tcttggtacg acatccacgt ggagcttccg ccctggtcgg ttgaggaggt
     4621 tcagcagtac ttatgcgacc gactccgtga ggctggcacc gatccggctg aggtcttcac
     4681 cctatcggcc atggaacgcc tcgccgaact tacgggaggc aatcctcgtg acgtgaaccg
     4741 actggccgag tggtgcctcc ttgtggcggc aggtgcggag gtgcggccga ttgggccgga
     4801 actggtgacg gatgtctttg aggagctata ttcgcaagtg ggcgacttag tcttaccacg
     4861 gtcaagccgc ggttgaatag tttgctccac gaatcgtcat actcaccgcg cggatgcccg
     4921 gtttaccacg ggttttccat catgccgacg atctgcatgg cgagccgttg gagagcggcc
     4981 tgttgggccg tggccaagga ttgaccgact tcggggacga gggttgtctc ggccacaacg
     5041 gtgctcagtt ccggcggaac agggatggct tcttccggcg tgaggaccct tccccgctgg
     5101 tcaatccatt gaacctcgac ctgcaagcgt gtggcgatct cgcggggatc gtcgtaggga
     5161 ttttcgacaa ccgtgcgttt ggtctcggat gtgatccgcc ctgtcaggat gctatccgcc
     5221 cgatccagcg agcctacgac cttgtaaggg gtacgcaatt cgatctcttt gatcacggct
     5281 tctgtcagtc gttctcctaa ccccgggcga aggctcgtcg attgaaagac cgggacatag
     5341 accgtctgaa tatccggccg gaagagtgtc gcatttccga ttcggtaccc ggcgcagccc
     5401 agtgttgctg tgaggatgat cagacttgtg ggcaccaagg caagccatgt caaaagaatc
     5461 caagcggagg gcgattgccg cggtcgatgc ctccgccggc agacgccgag cacgcgcggt
     5521 tccgttggct tgtgtcggtt aatcatcacg gcgttcaaaa aggtcggtca accacttgaa
     5581 gcggttggga ggtttatcgg gaagaccgtc aatctggcga atgcgttctg cggccatgac
     5641 ggcggccttg gtgtggggga actcccgcag tgttgcccgg tagtagtagc gggcggcccc
     5701 gtaatatttg cggttgtcat aatactgccc gatagcgaaa taccggaggg ccttttcctc
     5761 attgatttga ttcttgacgc ggatgacttc ctcccgctcg tctcctaact tgctgttata
     5821 gacggccagg atttcgtccg cggcctgctc ggcccgttcc aagggagttc catcatagag
     5881 tggcccttga taagtcaact gatgcgcttt gatggccaaa aaggccgcgt tgacagcatg
     5941 ttcactttgg ggatagtctt ttcgcaagag atcataatga taggctgcgt cctcgtatcg
     6001 ctgttttaga aaataggcgt tggcggtggc catgatggcg tcgtctgcca agggcccggt
     6061 gggatcgtgg agccgcacat tctgatacgc cttgatggcc cgtccccagg tgtcgaaggt
     6121 tggccgtgtt ttgtcaccga cttgaacgat acccaaaaat gacggtcgag aaagctccac
     6181 ctg
//
LOCUS       DAOPTR010000071         6143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_10498_length_6143_cov_3.144547, whole genome shotgun sequence.
ACCESSION   DAOPTR010000071 DAOPTR010000000
VERSION     DAOPTR010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10498_length_6143_cov_3.144547"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="PKI05_02365"
     CDS             complement(<1..838)
                     /locus_tag="PKI05_02365"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOA51071.1"
                     /translation="MSRHDDVPFAAELPESLKINRRHFIGGSTALAGIAGARLGAWAA
                     EVSADQPVPLKPIGQGKGIFPGRVVWVHDPGVVDWIGPDDGRWWEGDHVKQSRVNAMM
                     DQAVRHLTGADSVKDAWHRLFSHLNKTRKGAAQGYQAGQKILIKPNWVGMIYRENVVD
                     PETYRFIRRENYMNTSPQMLLALLRQLVDEVGVKPADITICDTLAYLVHEYYDILHAH
                     YPDVQYVDYAGKFGRIKVQASETPLYWSCRPQGVAQDFLPRCFAEADYLINFANFKAH
                     SGA"
     gene            1612..2364
                     /locus_tag="PKI05_02370"
     CDS             1612..2364
                     /locus_tag="PKI05_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51072.1"
                     /translation="MRLRKIDIRLEGPDIQPESIPVGELLDLLLCYERLLRDLTGKRH
                     SKVERKHREMRLRLVSVERGSTTLSCLAPEELYNANRTISQAIGKGSDLPPEARDSFF
                     RLRKRIEKLGCDVKIQGEDLEPFAISRERVPTKRFIRGETTVYGECIAVGGRKAVVKL
                     VSGSGDQIRIRLSNELAREIAKHLYEEIGVRGLATWEVSGPRIVDMEAYELLPYRRTS
                     PKEAIQALQESAGKDWGKIADPEEFLRKLRRE"
     gene            2378..2875
                     /locus_tag="PKI05_02375"
     CDS             2378..2875
                     /locus_tag="PKI05_02375"
                     /inference="COORDINATES: protein motif:HMM:NF025022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOA51073.1"
                     /translation="MGKRRSVILDTNILVYAIRPDDCNDDRAKRAKDFISDISRDDEA
                     DILIPAVVVAEFLCGCDSGFEDAVRNLQEHFKIVPFDMNAACQYRQVVTTRRRRSGSD
                     QDEAKQLAAAERNTVDEMIVATALAARAQELITYDQGLINLAKAVGIDAKEPPELGLG
                     LLPGF"
     gene            complement(3471..3544)
                     /locus_tag="PKI05_02380"
     tRNA            complement(3471..3544)
                     /locus_tag="PKI05_02380"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3508..3510),aa:Asp,seq:gtc)
     gene            4067..4210
                     /locus_tag="PKI05_02385"
     CDS             4067..4210
                     /locus_tag="PKI05_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51074.1"
                     /translation="MMSLQRYLDLAQHWIGELGPREWAMFAAMMLVIGLVCLRGLVAQ
                     RPY"
     gene            complement(4845..5669)
                     /locus_tag="PKI05_02390"
     CDS             complement(4845..5669)
                     /locus_tag="PKI05_02390"
                     /inference="COORDINATES: protein motif:HMM:NF018757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HOA51075.1"
                     /translation="MKRRICYLGDDDLSAAAAYLAGIMGHAGLPYAHVPSRRPVNGEI
                     LDEPYALYILSDYPSAGFSIEQMRRLAENVANGSGLLMIGGWESYFGRVGEYVNRPIA
                     DLLPVYLTTSDDRVNCPQGCYVRKARRHPITDGLPWQNPPMVAGYNRIVPKPDAQILL
                     VGERVWTVDTGKELQFSIVDTVPLLVIGKHGRGRVAALAFDVAPHWIGGMVDWGTRRI
                     VQQIPVGGFVEIGDDYAKFFRQLLAWTGRFEDMPDSYPEETTPREEVPLAPAPSKR"
     gene            complement(5798..>6143)
                     /locus_tag="PKI05_02395"
     CDS             complement(5798..>6143)
                     /locus_tag="PKI05_02395"
                     /inference="COORDINATES: protein motif:HMM:NF017922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HOA51076.1"
                     /translation="ARNLRALGTFWRPSHMPMPRPTSLRMDGPDLVIEWNDGTVARYS
                     PHRLRALCPCTSCAELRSHSTDPPSPDRFSNVTFRQVTPVGNYAYQITFSDGHNTGIY
                     TLDYLYRLRNLE"
BASE COUNT         1450 a         1581 c         1790 g         1322 t
ORIGIN      
        1 cagcgccact gtgggccttg aaattggcga aattgatcaa gtaatctgct tcggcgaagc
       61 agcgagggag aaaatcctgc gcgacaccct ggggacgaca actccaatac aacggtgttt
      121 cagaggcctg caccttaatc cgcccaaact tcccggcgta atccacgtac tgcacgtcgg
      181 gataatgggc atggaggatg tcgtaatatt cgtggaccaa gtaggccagc gtgtcgcaaa
      241 ttgtgatgtc cgccggtttg acgccgacct catcgaccaa ctgccgcagc agggcaagaa
      301 gcatctgcgg cgaggtgttc atgtagtttt cccggcgaat gaatcggtag gtttcaggat
      361 cgaccacatt ttcgcgatag atcatgccca cccaattggg cttgataagg attttttgcc
      421 ccgcctgata accctgggcc gctcctttgc gggttttgtt gagatgggag aacagccgat
      481 gccatgcgtc tttgacggag tctgctcccg tcaagtggcg aacggcttgg tccatcatcg
      541 cgttgacccg cgattgcttg acgtgatcgc cttcccacca tcggccatcg tcagggccta
      601 tccaatcaac aacaccggga tcgtggaccc agacaacccg cccggggaag ataccctttc
      661 cctgccctat gggcttgaga gggaccggct gatcggcgct gacctcggcc gcccaggcac
      721 ccaaacgtgc tcccgcgatt cctgccaggg cagtcgagcc tccaataaaa tgccgacggt
      781 taatctttag actttctggc aattcggcgg caaatgggac gtcatcatgg cgcgacatgg
      841 caaagcctcc ggaaaaaggg acattcattc caaacgctgc aatgttatgc tggagctatc
      901 ctggggcaga aatatttaac gtctttgagg gatgatcgct ccgctacccc cagctatgcg
      961 gcttttggca acacccataa atataaccgt ggcgggctcg aaatgcacca aacacccgcc
     1021 cgactccgcg ttccagcggc cgggctgaag gtgacatcgt ccacgtctcg aaccgccata
     1081 aggaacgccg ccccgccgat caggcgaaac accgatgacc ggggcaagca ggctggggct
     1141 tcattcgcca caactagtgg gcccgttgtc cacagaatag tgatgcgcta atgattcgca
     1201 tctttagtcg actggatttt ccacagctcg tactgcgaac caccgctccg ggatccgaaa
     1261 ccgaacgcat aagcccagag gagtcgagag tcgtcccatg ggacgagaat caaataaaaa
     1321 acgccggaaa aatccggcgt agggaacgag aagcgagggc gacggtgcga acaaattggc
     1381 gacaggaaag ggtaacctcc agatcgtggg agatagtctt cagcatagac gtcgggcagc
     1441 tgttaccgac caggatagac cccatcgcag tggggagatc gacaacacta gagtatcatt
     1501 gcgttggtct tggcgacggt ttagaattca tcgccgccag tgatatctgt gatctgcccg
     1561 gaagaggggc ttttcgcgct gatcggacgg cggagggctt aggctttcgg tatgagactt
     1621 aggaagatcg acatccgtct ggaaggcccc gatatccaac cggagagtat tcccgttgga
     1681 gagctgctcg acttgcttct gtgttacgaa cgacttttac gtgatctgac ggggaaacgg
     1741 cactctaagg tcgagagaaa acatcgagag atgcggcttc ggctggtatc agtggaaagg
     1801 ggaagtacca ccttaagttg tctcgctccg gaggaacttt acaacgcgaa ccgcacaatt
     1861 tcccaggcta taggcaaggg gtccgatttg ccaccagaag ccagggactc tttttttagg
     1921 ctccgaaaaa ggattgagaa gctgggttgt gatgtcaaga ttcaaggtga agacttggag
     1981 ccatttgcga tttcgcgtga gcgggtgccc acaaagaggt ttattcgggg cgagacaact
     2041 gtttacggag aatgcattgc ggtcggagga cgtaaggccg ttgttaaatt ggtgagcggt
     2101 agcggggatc aaattagaat ccgtcttagc aacgaattag ccagagagat tgcgaagcat
     2161 ctttacgagg aaattggtgt tcggggactg gcgacatggg aagtgagcgg accgcggatc
     2221 gtagatatgg aggcatatga gcttttgccc tatcgtcgga catcgccgaa agaggcgatt
     2281 caggcattgc aagagagcgc cggtaaggac tggggcaaga tcgccgatcc cgaggaattc
     2341 ttgaggaaac tcaggaggga gtaaaggggc gccctacatg ggaaagcgac gaagcgttat
     2401 cctcgatacc aacattcttg tatatgcaat ccgtcccgat gattgtaacg atgatagggc
     2461 aaaaagggcg aaggacttta tcagcgatat cagtcgggat gatgaagctg atatcttgat
     2521 ccctgcggtg gtggtcgccg aatttctctg cggttgcgac tctgggttcg aagacgcagt
     2581 gcggaacctt caggagcact ttaagattgt ccccttcgat atgaacgctg cctgtcagta
     2641 ccgtcaagtg gtgactacga ggaggagacg atcgggcagt gatcaggatg aggccaaaca
     2701 actggccgcg gcggagagaa atactgttga cgaaatgatc gtggccacgg cactagctgc
     2761 cagagcccag gaattgatca catacgacca gggacttatc aatttggcaa aggccgttgg
     2821 cattgatgcc aaggaaccgc cggagttggg tctaggtttg cttccggggt tttaggcgcc
     2881 aaacgaacaa cctttccggt cttactgcat ccgtgcgttc gagtaattgg cagtttgcgt
     2941 agctggcttg cgtttttttt ccaccgtgaa gcaagactgg gccctaagat cacgcaccgg
     3001 tttcagaaaa aagcggtaat ggttgctaaa gcagaatttg agcgatcctt catgcaccgc
     3061 ctcgtgggaa tttcttgaaa agtcgaagga aagaaagtct tagctgatct ttttctgaac
     3121 gttttgaagg cgtcggagaa ttatttcagc aggccagcgt ggatagttaa gcgcttggca
     3181 aacggcatcg gaagagagca ttcgccgtca tcgggataat gcaatagctc gtcagtggta
     3241 tactttaagc cgcttgggca tgtctttgtc tctgccagtt taaccaaagt attttctaaa
     3301 gcggtacttg tgacaaatca ccgctacggc gtttgccgtg tgccacaaag ttgtgctcca
     3361 tgatgcgaac cttcttttgg ggaacacgag tacagcaagg gtgatgccgt tttggaagtg
     3421 ttggaggaga acaaaaaaac gccggaaaat ccggcgtatg gaacgacaag cgagggcgac
     3481 gggactcgaa cccgcaacca ccggatcgac agtccagtac tctaaccaat tgagctacgc
     3541 ccccagcggt tggagcatca atataagtat aacccacggt caccctgaaa ggtcaatggg
     3601 gggaccttgc tccaagcggc tatctgcaag ccagatttca ctccagcagt acccgacaga
     3661 atgtccgtta aaaatggggc gcagactcgt agaaagcctg atcgcggacc tcggcaattc
     3721 gcttcgtcgt ccctacaggt tgggcagaat tgattaagaa gtggcccggc aaccccacca
     3781 ttagatagcc caaaacaacc acgatcgtgg aaagccccgg gggcgacggc cccggattgt
     3841 ttacgatttc tgcgaaaatt tttcgggggc attttgggaa aatgctatgt ttaagtagag
     3901 agtgctgtgt caaggtaact ggattaccaa aggatacacg actctcgatt ggtaggtctg
     3961 ggtttgggcg tgggacaaga agagttccaa gaggagggag gtctcggtcg gaaagtgtct
     4021 tcgggtcttt tctgaactgg ccgagctggc caaaaggtga agttttatga tgagcctcca
     4081 gcgttacctc gaccttgctc aacactggat tggtgaactg ggaccacgag agtgggccat
     4141 gtttgcggcg atgatgctgg tcattgggct agtctgcctg cggggacttg tcgcacagcg
     4201 gccctattga gcgtgcagaa gattttggac cagtggacta cgagcctatc ccataacctc
     4261 cccgctcccg tttggccgga gcccggtcgc gggtgagggt ggctcgaatt gctgccggtt
     4321 tttttactcg attctgatgt tcgcttgccc tcaccccaac cctctcccgg agggagaggg
     4381 ggactttttt agaatacgcc cttggtatag acggcccgac cggcatttgc aatacctaca
     4441 cggcgaggac gccgtttgca tgaaatcgga cggcggggac gccgaccccg gcgaccaaat
     4501 cacgtattcg cccgtagggg caaccccgcg tggttgccct gtttggctgg atggcgggtg
     4561 gtttgcgttt ggccgggcgc ccaggtcgtt tccaattcgc cctgacgggg ggcactcata
     4621 atgttccggg gcaggcacgg gggcctgccc ctacagggtt ttaatgggct gctattcgcc
     4681 caggacccca ggtcaatctt ccgcgcagca gcatcgctcg catacgaatg cacgcaggtg
     4741 ccacttgttg cccggaggtt ccggccacca gagagcgtga tctttgccgc ggtctagtgc
     4801 ataacacgca tgcatcgcta cgtcatttaa gaccacgcgg cgagctagcg ctttgacggt
     4861 gccggtgcca acggaacttc ttcccgaggg gtagtttctt cgggatagct gtcgggcatg
     4921 tcttcaaagc ggcccgtcca agccaacaac tgtcggaaga acttcgcgta atcatcgcca
     4981 atttccacga aaccaccgac cgggatttgt tgcacgatac gacgtgtccc ccaatcgacc
     5041 atgccaccaa tccaatgagg ggcgacgtca aacgccaggg cggccaccct gccccgcccg
     5101 tgcttgccga tgacgaggag cggaaccgta tcgacaatgg agaactggag ttccttacca
     5161 gtgtcaacgg tccaaacacg ctcaccaacc aagaggattt gcgcatccgg tttaggcacg
     5221 atgcgattat aacctgcgac catcggggga ttttgccagg gtagcccatc tgtaataggg
     5281 tgacgacgcg ccttgcggac atagcagcct tgtggacagt tgacccggtc atcggaggtc
     5341 gtcaaataga cgggcaacag gtcggcgatg gggcgattga cgtattcacc cactcgtccg
     5401 aagtaactct cccagcctcc gatcatcaaa aggcctgacc cgttggctac gttttctgcc
     5461 aaccgtcgca tttgctcgat ggaaaaaccg gcagaagggt aatcgctcag gatataaagt
     5521 gcgtagggtt catcgagaat ctccccattc actgggcggc gactggggac atgagcatag
     5581 ggcaagcccg cgtgtcccat aatcccggcc aggtaggccg ctgcagccga aagatcgtca
     5641 tcacccaaat agcaaattcg ccgcttcata gaatattgct cgctattcag aaaaaggaaa
     5701 agggcgaaac tctcaaggtt tgtgttttat tgtgaccagc ggtggacctt ccgacaagtc
     5761 aaatccatgg gccaccgtgt acccagccgg catggattta ctcgaggttg cggaggcgat
     5821 aaaggtaatc gagcgtgtag atgccggtat tgtggccgtc gctaaaagtg atctgataag
     5881 cgtaattgcc gactggggtc acctgtcgaa aagtcacgtt cgagaaacga tccggcgagg
     5941 gagggtcggt tgagtggctg cgcaattcgg cgcaactggt gcagggacac aaggcccgca
     6001 accgatgcgg gctgtaccgg gccacagtcc cgtcgttcca ttcaatgacc aggtcagggc
     6061 catccatccg cagactggtc ggtcgcggca tcggcatgtg ggatggcctc cagaaggtgc
     6121 ctaacgccct gaggtttcta gcg
//
LOCUS       DAOPTR010000072         6116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_10549_length_6116_cov_3.480449, whole genome shotgun sequence.
ACCESSION   DAOPTR010000072 DAOPTR010000000
VERSION     DAOPTR010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10549_length_6116_cov_3.480449"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PKI05_02400"
     CDS             <1..308
                     /locus_tag="PKI05_02400"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOA51077.1"
                     /translation="YQIADECNGPNARLHFRGSLSMANAGPNTNGSQFFITYAPTSHL
                     NGKHTVFGRVLSGMDVVEKLQARNPEAPNPPEPDKILSATVVRKRPHPYTVKKLEKS"
     gene            complement(358..663)
                     /locus_tag="PKI05_02405"
     CDS             complement(358..663)
                     /locus_tag="PKI05_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51078.1"
                     /translation="MIRSKSLSQSALSLWERVGVRASDRRSLSRKRAAIRATLTPGPS
                     PAERERGGCGIGSETAGKERPGEHSTQPGKKERYRLIVASVILIAWIISLMLMASLT"
     gene            complement(681..1139)
                     /locus_tag="PKI05_02410"
     CDS             complement(681..1139)
                     /locus_tag="PKI05_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51079.1"
                     /translation="MELLKEDPRYKLDAYLFIFQALDFARQLGMGREAPSEPLPDEVR
                     ERFREISGHVADEDEVEEEPPKHISGQELCEAARLYASQQFGYLAKTVLNEWGIYSTS
                     DFGEIVYNLIRIGLMRKTKEDRREDFDNVYDFEEAFCRNYRPGRGVGQFE"
     gene            complement(1418..1939)
                     /gene="aroH"
                     /locus_tag="PKI05_02415"
     CDS             complement(1418..1939)
                     /gene="aroH"
                     /locus_tag="PKI05_02415"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF019356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOA51080.1"
                     /translation="MMYCRGVRGATTADANTPEAILEATRQLLALMIRQNGIAPEDIA
                     AAFFSTSPDLNAEFPALAARQLGWMSVAMLCSHELDVPQSLQRCIRVLILWNTTKKAD
                     EIVHVYIKGAEKLRPDLSSLPPVDWQELEEWIAAHLNQEAQPSGREQGSSGGGVSPNL
                     GGADGIRGPSCAK"
     gene            complement(2046..4196)
                     /locus_tag="PKI05_02420"
     CDS             complement(2046..4196)
                     /locus_tag="PKI05_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51081.1"
                     /translation="MAIDRFSMCPGGTGKKIKFCHGDCVGFLEKVQKLMDGGQIRAAL
                     QLVDQTLSKDPSRSCVWSYKCLLERAVGNVDQFVKAADEYSQRFPKSPVALAELSLSH
                     LFRGQFSNAIDSLGQSLIASEEQKIIYVRQTAAAATLSEALIKFGHIPPAVEILMLFQ
                     NVSHNGQTSVQELLGQVIASQEIPLELREAQWDGLFMDEKYQQLLEVTDRLAVRLRWR
                     DLEKYLRDYLSSNPEDARVWHFLGVTLMWLMEPQEAATAFTRAGELEKNDRLAVFSLY
                     RSFLVTGALGDEENSYDVEVIVDEPDRLKEAVLSDRLVFSQRPEHGQPLPWYFPDDVG
                     EEITPELILAVLRRSIGGDGSQNGRVFSEPGKTPLIAFGSFYGRQTDRPARLLFAGVY
                     ESDLERLKEMVKGWLGREDVEWSIKPGSDESSRFIRLALDGEVIAGFDRSVDLKEEWK
                     TPIFEWLPNMTFPALGGLSLKEAANDPQKRRWALAILKYIDYRILNTTITGYLDELRQ
                     QLGLGFVDELDVDIKRFRRIPLSLVKVLDLTRLNIETLTQFFNLALVYRESTLIYELA
                     SEIVRRRDEDGVPAALVRTAYIGAIDEALNLGIDDDQIKRLTEEGLAFLEKHNLPHGS
                     LHVRQFRMAFSHGNSEEAKPLLDHMFREHEDEADVMEFLRQFMSLLGYFEQLAQQEGQ
                     SPTAGRPSGGGIWTPDSEAGRPGSSKKLWVPGSD"
     gene            4651..4869
                     /locus_tag="PKI05_02425"
     CDS             4651..4869
                     /locus_tag="PKI05_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51082.1"
                     /translation="MIKHHKWRDARRRIRRHTAAGFKLTVVPNRLHGLPLMHHVCFIP
                     LKGNLWLYAFFTLAPSREDLVDLPSWAE"
     gene            4923..>6116
                     /locus_tag="PKI05_02430"
     CDS             4923..>6116
                     /locus_tag="PKI05_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOA51083.1"
                     /translation="MFRMLSKAILLATVIFSLPGWSVASTEVQATRWNVIFILLDDLG
                     ATDFGCTGSRFYETPHIDRLAAQGMRFTQGYAACTVCSPTRAAVMTGKYPARLHITDW
                     IKGHVRPKAKLRIPDWTMYLPLEEVTIAERLRLAGYATAHVGKWHLGDPPYYPEHQGF
                     DINIGGTHMGAPGSYFWPYGKDDRRVPLDGGQEGEYLTDRLTDEAIRFIEQHKDRPFF
                     LYFAHYAVHTPLQAKPELVAYYEKKLAEAAAKGDNFDGQQCPVYAAMIHSVDENMGRL
                     LECLDRLDLAERTVIFLTSDNGGLVLPACQNRPVTHNIGIRAGKGSAYEGGVRVPWIV
                     RWPGVVAPGSLCHEPVISIDAYPTILEITGVADAEGHICDGVSLVPLLKQQGNFPKRA
                     LYWHYP"
BASE COUNT         1389 a         1777 c         1581 g         1369 t
ORIGIN      
        1 gctatcagat tgcggatgaa tgcaacggcc ccaacgctcg tctgcacttc cggggtagtc
       61 tcagcatggc caacgccggg ccgaatacca acggctcaca gttcttcatt acatatgccc
      121 caaccagcca cttgaacggt aaacacaccg tctttggtcg tgttctctca ggaatggatg
      181 tggtggagaa acttcaagcg cggaatcccg aagccccgaa tccaccggag cccgacaaga
      241 ttttatccgc gactgtggta cggaaacgtc cccatcccta cacggtgaag aaactcgaaa
      301 aatcgtgatc tactgaccac gccagacctg gttcgttcgc tgatggtcag tctccgccta
      361 tgtgagactg gccatcagca tcaggctgat gatccaggcg atgagaatga ccgaggcgac
      421 aatcaaacgg taccgctcct ttttgccggg ctgagtcgaa tgttcgcccg gtcgttcttt
      481 ccccgctgtc tcagagccta tcccacaacc tcccctctcc cgttcggcgg gagaggggcc
      541 gggggtgagg gtggctcgaa tcgctgcccg ttttctcgat agactccgac ggtcacttgc
      601 cctcacccca accctctccc agagggagag ggcactttgg gataggctct tggaacgaat
      661 catcttcgcc actccggggt tcattcaaat tggcctactc ccctaccggg acggtagttt
      721 cggcagaagg cctcttcaaa gtcgtaaacg ttgtcaaagt cttcgcgtcg atcttctttg
      781 gtttttcgca tgaggcctat tcgaatcagg ttatagacga tctcgccgaa atcactcgtg
      841 gagtagattc cccattcatt gagcacggtc tttgcgagat agccaaattg ctggctggca
      901 tacagcctgg cggcctcgca aagctcctgg ccggaaatat gcttcggcgg ttcctcctcc
      961 acctcgtctt catccgcgac gtgaccactg atctcccgaa agcgctcccg gacctcgtcg
     1021 ggcaagggtt cactgggcgc ttcacgcccc atgcccaatt gccgcgcaaa atcgagtgcc
     1081 tggaagatga agagataggc atccaacttg tagcgtggat cttccttcaa aagttccaag
     1141 agcgctcgtg gtaaaggcga cgcgtgtccg gccatggctt cttgacaacg tgtattaaag
     1201 cgtatcccac aacctcccct ctcccgttcg gcgggagagg ggccgggggt gaggatggct
     1261 tgaatcgctg gcgttttctc gatcgactcc gacgatggct tgccctcacc ccaaccctct
     1321 cccaaaggga gagggaattc tgaaataggc tcattagcaa acggctctca gtgtttcaaa
     1381 gtagcttaca tctttttttc ctatacgcag cgtccggcta tttggcacac gacgggccgc
     1441 ggataccatc ggcgccgccc aaattggggg aaacaccccc acccgaggag ccctgctccc
     1501 gaccgcttgg ttgggcctcc tgattgagat gggccgcgat ccactcttcg agttcttgcc
     1561 aatcgacggg cgggagcgaa gagaggtcgg gccgcagctt ttccgcaccc ttaatataca
     1621 cgtgcacaat ctcgtcggcc ttcttggtcg tgttccaaag gatcaacact cggatgcacc
     1681 gttgcaggga ttgggggaca tcgagttcgt gactgcataa catggcgacg ctcatccaac
     1741 ccaactggcg ggcagccagg gctgggaact cggcatttaa gtcaggagaa gtcgaaaaaa
     1801 aagcagccgc aatgtcctcc ggagcaattc cattttgtcg aatcatgagc gccagcaact
     1861 gtcgcgttgc ttccaaaatt gcctctgggg tattggcatc ggcagtcgtt gcgcctcgca
     1921 cgcctcgaca gtacatcatg gttcgcaact tacctccaag gacccaattc cacaagccaa
     1981 cgtgacgact gatccagact caagccacgc catcatacgg ccccgttggc attttccggc
     2041 agatgtcaat cgcttcccgg cacccagagc ttcttcgatg agcctgggcg accggcttcg
     2101 gaatcagggg tccaaatccc tccaccggat ggacgtcccg cggtcgggga ttgaccttcc
     2161 tgttgcgcga gctgttcaaa ataccctaga agcgacatga attgccgaag gaattccatg
     2221 acatccgcct catcctcatg ttccctgaac atgtgatcga ggagcggctt tgcctcttcg
     2281 gaattaccat gcgagaaagc cattcggaac tggcggacat gtaaactacc gtgcggcaag
     2341 ttgtgtttct caagaaacgc caagccttct tccgtcaggc gtttaatctg gtcgtcatcg
     2401 atcccaaggt ttaatgcctc atcaatcgcc ccgatataag cagttcggac aagggccgca
     2461 ggaacaccgt cttcgtcccg tcgccgcaca atttccgagg ccaactcata aatcaaggtg
     2521 gactcacggt aaaccaaagc cagattgaaa aactgcgtca gcgtttcaat attgaggcgc
     2581 gtcaagtcta ggacttttac taacgacagc ggaatccggc ggaatcgctt aatgtcaaca
     2641 tcgagctcat ccacaaagcc caagccaagt tgctgtctca actcgtccag gtaaccagta
     2701 atcgttgtgt ttaagattcg gtaatcgatg tacttgagaa tggccaaggc ccaacgacgc
     2761 ttctgcgggt cgttcgccgc ctctttcagc gagagaccgc ccaacgccgg aaacgtcata
     2821 ttgggcaacc attcgaaaat aggggtcttc cactcttcct tgagatcaac ggaacgatca
     2881 aaacccgcaa tgacctcccc gtcgagcgcc aatcttataa aacgagaaga ctcgtccgac
     2941 cctggtttta ttgaccattc gacatcctcg cgacccagcc accctttgac catctccttg
     3001 agtcgctcca aatcggattc gtaaactccc gcgaaaagca atcttgcggg ccgatctgtt
     3061 tgccgcccgt aaaaactgcc gaaagcaatc aggggcgtct tgccgggttc gctaaacacg
     3121 cgtccatttt ggctgccatc ccctccgatt gaccgacgca aaacggcaag gataagctcc
     3181 ggagtgattt cctcgcccac atcatcgggg aaataccatg ggagcggttg cccgtgttcc
     3241 ggtctttgcg agaaaaccag acggtcggaa agcacggcct ctttcaaccg atctggttca
     3301 tcaacgatga cttccacgtc gtagctattt tcttcgtcgc ctaatgcacc ggtcacaagg
     3361 aaagaacgat acagagagaa gactgccagc cgatcgttct tttccaattc gccagcgcgg
     3421 gtaaaggccg tggcggcctc ctgaggttcc atgagccaca tcaacgtcac gcctaagaaa
     3481 tgccacacgc gggcatcttc tgggttacta ctcagataat ctcgcagata tttttcgagg
     3541 tcccgccatc gtaaacgcac agccagacga tcggtgacct ccaaaagctg ctgatatttc
     3601 tcatccatga acaagccatc ccactgggcc tcccggagtt ccagcggtat ctcttggcta
     3661 gcaataacct ggcctagtaa ctcttgcaca ctggtctgcc cgttatggct gacgttttgg
     3721 aaaagcatga ggatttccac agcgggcgga atgtgaccaa acttaatgag cgcctcgctc
     3781 agagtcgccg cagccgccgt ttgcctgaca taaatgattt tctgttcctc gctggctatc
     3841 aagctttggc ctaaggagtc tatggcattt gagaactggc cccggaaaag gtggctcagg
     3901 gacaattcgg ccaaggccac gggacttttc ggaaatcgct ggctgtactc gtcggcagcc
     3961 ttaacaaatt ggtccacatt cccaactgcc cgctccaaaa gacacttgta ggaccaaacg
     4021 caggaacggc tcgggtcttt ggacaacgtt tgatcaacca gttggagggc cgctcggatc
     4081 tgcccaccat ccatgagctt ctggactttt tccaagaagc ccacacaatc gccgtggcaa
     4141 aacttgatct ttttacccgt tccgccggga cacatcgaaa acctgtcaat agccatgatt
     4201 gggatatctc ctcatccgaa ctttttcacg gatcgaaaaa atttcgcaag cgtccaccta
     4261 ggccccgcct aggtgttaag gacagcccgc tgctgtcaat ttcaaccagc gcttttgtca
     4321 gaaaacgcga ccctccacaa cacgtgaaat ccaaacaaac cggtaaatct tttcacattt
     4381 ggcttggggc cccgcaaaag gccaagcctt tatgttatca ggtattgcct ggcgtgggga
     4441 gacaatgagc gggccgggac gccatttccc ggcagatcgc ctgtgagaag gggcatacac
     4501 tcctaacgcg caggcgcggg gccccgccct ggcgagaacc ggaaaacgcg tccatataaa
     4561 gtgccccacc ttatggcggg gtccgcaatt gcgatgtagg ttcccggtgt ttgacaggac
     4621 catgacggtg cgggcccgtc agggtgaacg atgatcaaac atcacaaatg gagggacgca
     4681 cgccgtcgca tccgccgtca tacagccgcc ggttttaaac tgacagtggt tcccaaccga
     4741 ttgcacgggc tcccattgat gcaccacgtg tgttttattc cccttaaggg caatctttgg
     4801 ctctacgctt tctttacgtt ggcaccatca cgggaagacc ttgtggactt gccctcttgg
     4861 gcggagtaaa ctagggcaaa cgagaacagc tcgatcattg agtccgcttg gggaggtaga
     4921 acatgttccg aatgctctca aaagctattt tgttggccac ggtcatcttc agcttgcctg
     4981 gctggtcagt ggcgtcaaca gaagttcaag cgacgcgctg gaatgtgatt ttcattctgc
     5041 ttgatgactt aggggccacc gatttcggat gcacaggcag tcgcttctat gagactcccc
     5101 acattgatcg gttggctgcc cagggaatgc gattcaccca gggttacgca gcctgcactg
     5161 tttgctcacc aacgcgggcg gccgtcatga cgggaaaata tccggcacgg cttcatatta
     5221 ccgactggat caagggacat gtccgtccca aggcaaaact gcgcatcccc gattggacaa
     5281 tgtacctccc tctcgaggag gttacgatcg ccgagcggtt gcgtttggcg ggttacgcca
     5341 ccgcccacgt cggcaaatgg catctgggcg atcctcccta ttatcctgaa catcagggat
     5401 tcgacatcaa catcggcgga acacatatgg gagcccccgg tagttatttc tggccgtacg
     5461 gcaaggacga tcgtcgcgtg ccgcttgacg gggggcagga gggggaatat cttaccgatc
     5521 ggctaacgga cgaagctatc cgctttatcg aacagcacaa ggatcgaccc ttttttctct
     5581 atttcgccca ttatgccgtt catactccgt tgcaggccaa accggaatta gttgcctatt
     5641 atgagaaaaa gctggccgag gccgctgcca aaggggacaa ttttgacggg cagcagtgcc
     5701 cggtttatgc agccatgatt cacagtgtgg acgagaacat gggccgcctc ttggagtgct
     5761 tggatcgact cgacctggcc gagagaaccg tgatttttct cacgtcggac aatggtggat
     5821 tggtactgcc ggcttgtcag aacaggcccg tcacgcataa catcggcata cgggcaggta
     5881 aaggctcggc atacgaagga ggtgtccgcg tcccatggat cgttcgctgg cccggtgttg
     5941 tcgctccagg aagcctttgc cacgaaccgg taatcagcat cgacgcctac cccactattt
     6001 tggagatcac gggggttgcc gatgccgagg gtcacatctg cgatggcgtc agtcttgtgc
     6061 cgcttctgaa acaacaaggg aacttcccca agagggccct ttattggcac tatccg
//
LOCUS       DAOPTR010000073         6108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_10560_length_6108_cov_3.063605, whole genome shotgun sequence.
ACCESSION   DAOPTR010000073 DAOPTR010000000
VERSION     DAOPTR010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10560_length_6108_cov_3.063605"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /gene="trpA"
                     /locus_tag="PKI05_02435"
     CDS             complement(<1..612)
                     /gene="trpA"
                     /locus_tag="PKI05_02435"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOA51084.1"
                     /translation="MSRISEVFKALRKEGKRAFMPFLTAGDPNLDFTVRLMHTLADEG
                     CSLLEIGIPYSDPIADGPVIQASYTRALTSGIRLSDIFRTFSQTLPKLPVPAVAMVSY
                     AIVLRQGLGAFLDRAKDCGLAGLIVPDLPIEECGELRERCLANGLDLVQLVTPTTPRN
                     RAVAIAQLSTGFLYYVSVAGITGERNELPPSVVENVTWLRQQTA"
     gene            complement(682..1884)
                     /gene="trpB"
                     /locus_tag="PKI05_02440"
     CDS             complement(682..1884)
                     /gene="trpB"
                     /locus_tag="PKI05_02440"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOA51085.1"
                     /translation="MRVTTNVPDQQGRFGKFGGRFVPETLMFALDQLTEAYEKAIADP
                     EFHQQLEYLYRHYVGRPSPLYFAERLSRLCGGAKIFLKREDLNHTGAHKINNALGQAL
                     LTIRMGKRRVIAETGAGQHGVATATACARFGLECVVYMGEEDIRRQRPNVFSMRLLGA
                     TIIPVTSGSKTLRDAINEALRDWMSSVETTHYCLGSVTGPHPFPRIVRDFQSIIGRET
                     RAQCLEQIGRLPDWIVACVGGGSNAAGMFYPFIEDDVALLGVEAGGRSPQLGDHAATL
                     SYGQPGVLHGTFSYVLQDEAGQTANVHSISAGLDYPGVGPEHSYWKETGRVTYTSVTD
                     QQALEAFQTLARYEGILPALESAHALAKAMELARSLPPEKVIIVCLSGRGDKDTQEVA
                     RLLGQQFA"
     gene            2131..2598
                     /locus_tag="PKI05_02445"
     CDS             2131..2598
                     /locus_tag="PKI05_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51086.1"
                     /translation="MTGSDTFLDPGFYELVHQILSNSQRRVLRERRIRPRFSFDVKQR
                     IAPWTNSRPEEKDFIPVRCFDLNTNGVAFFLEEPPSFRRLIVELSCPQRKVYLESEVA
                     HWEYVYLYPCGDIVPEEVVNELDEEERRDLGEAQRLVLVGCRFLARSKLCHEK"
     gene            complement(2734..5454)
                     /locus_tag="PKI05_02450"
     CDS             complement(2734..5454)
                     /locus_tag="PKI05_02450"
                     /inference="COORDINATES: protein motif:HMM:NF019935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOA51087.1"
                     /translation="MVPSTHDGSRSLVRDGLAPHVKAFRDRLWGESQAAISEEYSPGK
                     GLQAALSLCRLRDHILHQLWKIHSSDKADAVWNEETDFYAVMALGGYSRGTISLHSDV
                     DLLILTTRREPSGWKGFISSYFRDIYDLGLKLGFLQLRARAALQKILQDPHFCTAVLS
                     ARFLWGHRPVFEAFQEAIRNKLRRNLKRVLASIERARCEERDRFGKTVFLLQPNIKRS
                     PGGLRDIQFIDWIRYFWELWEPVSSIPITDVLLREELEALSDTEDFLLQIRHLLHLWA
                     NRPEDVFSRSDQWLLAERWHCQATVNLLPVEVLMRDYFRRTEQVVLVAKRLAHALEHQ
                     LTPPKGIAKRSKDRTLVEIKGRLWLTESGRETLKRGWAGLFDLFEACQQLDLPFDASV
                     WIEIWKFRSLFAAPPTPEIRQRFLQLLQSPHQTASLIRGLHQVRLLENFLPDMEHARG
                     LLQFNQYHKYTVDEHCLNALEAATALAYQPGLLGETYRKTINKKILHLAIILHDLGKG
                     LAADHSEAGRSIARRTAEYLALEPDDAELLEFLVANHLRMNHLALRRDINDEQLIVQF
                     AAEIGSPDVLRMMYILTATDLMAVSPDNWTAWKADLLAELYQRTMRYLSDSGDVILTD
                     LDLQERRKAVENALGHDVARPFFSRQVRELPAGYLLATDVETIVRDLRWLASLGNCPV
                     DVRIEYRPSSQTLNLTIATRENVIEGIFHRLTGAITSCGLEILAAQIYTLADGWIIDR
                     YTVRDPYFVGEPPLDRYQDIRTAIVSALREKQFQPVFPKIWGMSPSSRVQVPQARVRV
                     QIDNVSHSDFTLIEVFANDRPGLLYAISRELFMAGCFIWRAKIGTFLDQVVDVFYVTD
                     QKGQKLLDPEHVQILRANLLQVVQSGWEEGSDTPAGSSSNPPTVRAHHDP"
     gene            complement(5457..5798)
                     /locus_tag="PKI05_02455"
     CDS             complement(5457..5798)
                     /locus_tag="PKI05_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOA51088.1"
                     /translation="MKKVEAIVRHFKLEDIKAALMEIGIQGMTVTEVRGFGRQKGHTE
                     TYRGTEYSVDLIPKVKIEVVVPDADLEQVLESIIQAAQTGQVGDGKIFVSDVSQAIRI
                     RTRETDDAALR"
     gene            complement(5845..6027)
                     /locus_tag="PKI05_02460"
     CDS             complement(5845..6027)
                     /locus_tag="PKI05_02460"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51089.1"
                     /translation="MALLLFYAIKTTVGLRVKPETELAGLDISEHGILAYPEAFVVDT
                     GMPLGGSAALAQPRKK"
BASE COUNT         1430 a         1603 c         1693 g         1382 t
ORIGIN      
        1 tgctgtctgt tgccgcagcc aggtgacgtt ctcaacgaca cttggcggaa gttcattgcg
       61 ttcccctgtg atgccggcca cggagacgta ataaagaaag cccgtgctca gttgggcaat
      121 tgcaactgcc cgattgcgcg gtgtggtggg tgtgaccaat tggaccagat caagcccatt
      181 ggccaggcaa cgttcccgta attccccgca ttcttcaatg ggtagatcag ggacaattaa
      241 tcccgcgagc ccacaatctt tcgcccggtc caaaaaggcg cccaacccct ggcgtagcac
      301 gatggcataa ctgaccatgg caactgcggg cacggggagc ttgggtagcg tttggctgaa
      361 cgttcggaaa atgtcggaga gccgaattcc cgaggtaagg gcccgcgtgt atgaggcttg
      421 aatgactggg ccgtcggcga tggggtcact gtagggaatg cctatttcta aaagggagca
      481 cccttcatcg gccagggtgt gcatgagtct aacggtgaag tccagatttg ggtcgcctgc
      541 cgtcagaaat ggcatgaacg cccttttacc ttccttccgc aaagctttga acacttcact
      601 gatcctagac atataatgct gcttttcccg tgcagtcatc ttattgaaag ctggtgtgtc
      661 tttcttttgg tctcaaatca cttacgcaaa ttgctggcca agaagtcgcg cgacctcctg
      721 agtatctttg tcaccgcggc cagagagaca gacaatgata accttctcgg gcggcaacga
      781 gcgtgcaagc tccatggcct tggccaaggc gtgggcactc tctagagcag gaaggattcc
      841 ctcgtatcga gccaacgttt gaaatgcctc gagggcctgt tggtctgtga cactggtgta
      901 ggtcacacgt ccggtctctt tccaataact gtgttccgga cccacgccgg ggtaatcgag
      961 acctgccgag atcgaatgga cattggccgt ctgtccagcc tcgtcttgaa ggacatagga
     1021 aaaggttcca tggagaacac ctggttgccc ataggaaagg gttgccgcat gatcacccag
     1081 ttggggcgaa cgcccaccag cttcgacccc caagagggcg acatcgtcct cgatgaaggg
     1141 ataaaacatc ccggctgcat tgcttccacc accgacacaa gccacaatcc aatctggcaa
     1201 gcgtccgatt tgctccaggc actgtgcgcg ggtttctcgt ccgatgattg actgaaagtc
     1261 gcggacgatc cttgggaagg gatgagggcc ggtgaccgat cccagacaat aatgagtcgt
     1321 ttcgacagaa ctcatccagt cgcggagtgc ctcattgatg gcgtccctaa gggtcttgga
     1381 accgcttgtg accgggatga tcgtggcccc gagaagtcgc atgctgaaga cgttgggacg
     1441 ctgccgccgg atatcttcct cgcccatgta aacgacgcat tctagcccaa aacgggcaca
     1501 tgccgttgcg gtggcaacgc catgctgacc cgcacccgtc tccgcgatca cgcggcgctt
     1561 tcccatgcgg atcgtgagca gggcctgtcc cagggcatta ttgatcttgt gggcacccgt
     1621 gtgatttaaa tcctcccgct tcagaaaaat cttggcaccg ccgcagagtc tcgacagcct
     1681 ttcagcgaaa taaaggggag aggggcgacc gacgtagtgc cggtaaagat actccaattg
     1741 ctggtgaaat tcggggtcgg caatggcctt ctcgtatgcc tcggtcagtt gatcgagcgc
     1801 aaacatcaag gtttcgggca caaatctgcc cccgaacttt ccgaatcgcc cctgctgatc
     1861 agggacgttt gttgtaactc gcatgggaga atcgccttta atccacacaa caaccttatc
     1921 aaggaccgaa aaaaccaatt ataacacttg tgcagcacgc caaaccaaaa atcttatcgt
     1981 aggttagaat tgagatgtca atcaaggcgt catcgaggcg gtgagagttg aaagatcggc
     2041 cgggcatggg tgactttgcc aaatagaaca cactcgacat atgggatgat cgtactttcc
     2101 gggaaatacc gtgcggggac atgaaaagct atgaccggat cggatacgtt tcttgacccc
     2161 gggttctatg agcttgttca ccagattctg tcgaactccc agcgacgggt gcttcgagag
     2221 cggcgaattc gtccccgttt ctcgtttgat gtgaagcagc ggatcgcccc gtggacgaac
     2281 agcaggccgg aggaaaagga ttttatcccc gttcgttgct tcgatcttaa cactaatggt
     2341 gtcgcctttt ttctcgaaga gccgcctagt ttccgacgac tcatcgtcga gctgagttgt
     2401 ccgcagcgga aggtctactt ggaatcggaa gttgcccatt gggaatacgt ctatctttat
     2461 ccgtgcggtg acattgtgcc cgaagaagtg gtgaatgaac ttgatgagga agagcgacga
     2521 gacctgggag aagcacaacg tcttgtcctc gtcggctgcc gcttcctcgc tcgatccaag
     2581 ctgtgccacg aaaaatagac gcacaagtgg tgggcccatg aggccgcgtt cctgagttgt
     2641 cccatcgagt agggggagcg gggcgccgat ctttggtcaa gaacaaggat gggagatttc
     2701 catcattttt agccctcaca gtgaggcgat gtgtcacggg tcatgatgcg cacgaacggt
     2761 aggtgggttg gaagacgaac ccgcgggggt atctgagcct tcttcccagc cagattgcac
     2821 tacctgaagg agattggccc gaagtatctg cacatgttcc ggatcgagca atttctgccc
     2881 cttttgatct gtgacgtaaa agacatcgac cacctgatcg agaaaggtcc caatcttggc
     2941 acgccagatg aaacagccgg ccatgaacag ctctcggcta atggcatata gcagcccagg
     3001 gcgatcgttc gcaaagacct cgattaacgt gaagtcggag tgactaacgt tgtcaatttg
     3061 gacgcgaaca cgcgcctgag gtacctggac acgcgacgat ggggacatgc cccatatttt
     3121 tggaaaaacg ggctggaact gcttctcccg aagtgctgag acgatcgccg tacgaatgtc
     3181 ctggtaacgg tccaaaggag gctcgcccac aaaatacggg tcgcgcaccg tgtaccggtc
     3241 gatgatccag ccatcagcca aggtgtagat ttgggcggcc agaatttcta gcccacagct
     3301 tgtgatggcc ccggtcaatc tgtgaaaaat tccctcgatg acattctctc gagtggcaat
     3361 cgtcagatta agggtctggc ttgatggacg atattctatg cggacatcca caggacagtt
     3421 gcccaacgat gcaagccaac ggagatcacg aacgatcgtc tcaacatccg tagctaggag
     3481 ataacccgcc ggtagttcac gtacttggcg tgaaaagaac ggccgagcga catcatgtcc
     3541 cagggcgttt tcgacagcct tccgccgttc ttggagatca aggtctgtga ggatgacatc
     3601 gccagaatcg gaaaggtacc gcatcgtccg ctgatatagc tctgccaaca agtcagcctt
     3661 ccatgccgtc caattgtccg gtgaaacggc catcaagtcg gtggccgtca gaatatacat
     3721 catgcgcagc acatcgggtg agccgatttc cgcagcaaac tggacgatca attgctcatc
     3781 gttgatgtca cggcgaaggg ccaggtgatt cattctgaga tgattggcga caagaaactc
     3841 cagtaattcg gcatcgtcgg gctcgagggc gagatattcg gccgttcggc gggcaatcga
     3901 tcgcccggcc tcgctgtggt cagccgctaa acctttgcca aggtcgtgca gaataatggc
     3961 caaatgaaga atctttttgt tgatggtctt tcgatatgtc tcacccagca ggcccggttg
     4021 ataggccaaa gcggtcgccg cctcgagggc gttcaggcag tgctcatcga ctgtgtattt
     4081 atggtactga ttgaactgca aaaggccgcg agcatgctcc atgtcgggta gaaaattctc
     4141 caagaggcgg acttgatgga gcccacggat gagtgaggcc gtctggtggg gtgattgcaa
     4201 aagttgtaag aatcgctgcc ggatttcggg tgtgggcggc gcggcaaaaa ggcttcggaa
     4261 tttccagatt tcgatccaca cgcttgcatc gaagggcaaa tcaagctgtt gacacgcctc
     4321 gaaaagatcg aagaggcctg cccagccacg ctttagcgtt tctcggccgg attccgtcaa
     4381 ccacagtcgt cctttgattt caacaagtgt gcgatccttg cttcgtttgg caattccctt
     4441 gggtggtgtc agttggtgct ctaaggcatg tgccagtctt tttgccacga ggacgacctg
     4501 ttctgtgcgg cgaaaatagt cacgcatgag cacttcgacg ggaaggagat tgaccgttgc
     4561 ttgacagtgc caccgctctg ccaggagcca ttgatcgctg cgcgaaaaga cgtcctcggg
     4621 gcggttagcc cacaggtgca agagatggcg gatttgtaag agaaaatcct ccgtatcgct
     4681 tagcgcctct agctcttcgc gcaataggac gtccgtgatc ggaatcgacg acaccggttc
     4741 ccaaagctcc caaaaatacc tgatccagtc aataaactgg atatcacgaa ggccaccagg
     4801 tgatcgtttg atgttgggct ggagtaagaa gacggtcttg ccaaatcgat cacgctcttc
     4861 gcatcgggca cgttcgatag aggcgagtac tcgcttgagg ttacgtcgta acttattacg
     4921 gatagcctcc tgaaaggctt caaacacagg gcgatgcccc caaagaaaac gggcagacag
     4981 aactgctgtg caaaaatgag gatcttgcag gatcttttgc aaagccgcac gggcacgcaa
     5041 ttgtaggaat cctagtttca gtccgagatc atagatgtcg cgaaagtagg aagagataaa
     5101 acctttccac cccgagggtt cccgccgggt ggtcaaaatc aagaggtcca cgtcggagtg
     5161 aagagaaatc gtgccccggc tataaccgcc taaggccatg acagcgtaaa agtccgtctc
     5221 ctcgttccaa accgcgtctg ctttatccga tgaatgaatc ttccacaatt gatgtaagat
     5281 gtggtcgcgg agacggcaga gagacaatgc cgcttggagg cctttacctg gggaatattc
     5341 ctccgagatt gccgcttggc tttctcccca gaggcggtcg cgaaatgcct tgacgtgagg
     5401 cgctagccca tcgcgtacaa ggctacgaga gccatcatgc gtcgatggga ccatggctac
     5461 ctgagggctg cgtcatcggt ttcgcgtgtc cttatccgaa tggcttggga cacgtcactg
     5521 acaaatatct taccatcccc gacttgcccg gtttgtgccg cttgaatgat gctctcaagt
     5581 acttgttcca ggtcggcgtc ggggacgacg acctcgatct tgacttttgg gatgaggtcc
     5641 acgctgtatt ccgtaccgcg gtaagtctcg gtgtgtcctt tttggcgacc gaaaccgcgg
     5701 acctctgtga cggtcatgcc ctggatgccg atctccatga gcgccgcttt gatgtcttcc
     5761 aatttgaaat gcctgacgat tgcttctact tttttcatat tgcagttacc tcacatgctt
     5821 tttgtggaag accattgaac acaatcactt cttacggggt tgggccaatg ccgccgaacc
     5881 tcccagaggc atccctgtat caacgacgaa tgcctcggga taggcgagaa tgccgtgttc
     5941 actgatatcg aggccggcca gctctgtctc tggtttgacc cgaagtccta ccgtagtctt
     6001 gatcgcgtag aaaagcaaca gcgccatgcc gaaagaccat taacaatgag cgttttcggg
     6061 aaaaacagca aatcttccga aaaacaggga aaacaggtgt gaagacga
//
LOCUS       DAOPTR010000074         6107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_10562_length_6107_cov_3.008096, whole genome shotgun sequence.
ACCESSION   DAOPTR010000074 DAOPTR010000000
VERSION     DAOPTR010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6107
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10562_length_6107_cov_3.008096"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /gene="nadC"
                     /locus_tag="PKI05_02465"
     CDS             <1..798
                     /gene="nadC"
                     /locus_tag="PKI05_02465"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOA51090.1"
                     /translation="RKRGILAGVLTVEVILAELSLDVQWKSLLTDGASLEKGTVIGRL
                     QGRVRDILSVERILLNFVGRLSGIATLTHSYVSAVRGTKARIYDTRKTTPGWRRLEKY
                     AVRCGGGHNHRLGLDRAILIKDNHLAWLAEELQCSLEDAAKIAIRRARELYPGHPIPS
                     GQDPADIIVEIEVDSLPQLETVLCENPDIVLLDNMSLEELRQAVLLRNQLAPHVELEA
                     SGGVTLENVRAVAETGVDRISVGALTHAAPWLDIGLDWGADGSGAVE"
     gene            complement(900..1322)
                     /locus_tag="PKI05_02470"
     CDS             complement(900..1322)
                     /locus_tag="PKI05_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51091.1"
                     /translation="MKSVQNASRIVISFLLAGAIFWATGCGSGGPPPKKDDSRNPASH
                     AQYIKNLTIRTLKKVPNLTKDEAVGLLQNMRAGLAQYEDVPMGEFEATYREIVKGTEE
                     LLELCRQGGDKAQIKSRADALIEKANTLPGELEEETTG"
     gene            complement(1391..2425)
                     /locus_tag="PKI05_02475"
     CDS             complement(1391..2425)
                     /locus_tag="PKI05_02475"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOA51092.1"
                     /translation="MKKALVVRWSRGFTLVELLVVIAIIGILIALLLPAVQAAREAAR
                     RNQCTNNMKQVVLALHNYHDINKQFPPAALGCTGAGFPYRALDTAAMAGKQPRNTLDT
                     WGATWITMLLPQLEQQALHSNYDFRRPCIDPVNQPVVKTQIATLICPSDASDNRVFIR
                     ATGQEFAKGNYVANCSARRYMSDLDGYTTLEYRGVFNTWVQWGAKIESILDGSSNTVA
                     ISEVCTSPETADARGAWGHVSGVAFGGGISGGSTPKGTLTPNANALDDNYRDLIEQYC
                     KAPNNDRRLRCATPGNAWDARIGARSYHPGGVNVGMADGSVRFIQETIDVTTWGALLS
                     SRGNEAVTLP"
     gene            2730..3902
                     /locus_tag="PKI05_02480"
     CDS             2730..3902
                     /locus_tag="PKI05_02480"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HOA51093.1"
                     /translation="MPRFEHDRLPRIALLIETSNEYGRRLLLGIRDYVREHEPWSFQL
                     GEQARGHTVPSWLSKWKGDGIIARIDNASIEQAVREVGVPVVNVSASGFGLAFPTVIS
                     DSEAIAQTAADHLLECGFSQFGYCGDPRFAWSKSHERHFKQYLQQRGFSCHCFSGSKS
                     AANHWGRALEELIGWVRELPKPIGIMACYDLRGQQLLATCREAGIHVPDEVAVIGQHN
                     DETICEFCDPPLSSVIPNPRLAGYLGASLLDRMLKGEWIPGGVYRVPPIGIAVRRSTD
                     VVAVPDEDVSAAIRYIRDHACQGIDVRDVVHAVNISRSMLERKFKRYLNCNPHDLIVR
                     TQLRRAQELLIQTDLTIENVATLSGFSSAEYMSCVFHRRVGQSPRDFRKRCRGKLL"
     gene            4117..6039
                     /locus_tag="PKI05_02485"
     CDS             4117..6039
                     /locus_tag="PKI05_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HOA51094.1"
                     /translation="MTMRRLVAVWVGLFLAVVLPLVRQVPRALGESGGHGDGRVTISG
                     ELKRWHKVTLTLAGPFARERDESPNPFTDYDFTVEFRHESGEPVYRVPGYFAADGQAA
                     ETSAEAGNCWRAHLAPDKTGVWRYRVSFLKGKHAAIGGKGQPLAPYHGVSGQFTVAET
                     DKTGRDFRAKGRLAYVGKHHLQFLGTGEFFLKAGADAPETFLAYADFDGTIATKKNVP
                     LHKWEPHIRDWQPSDPSWKNGKGKGMIGALNYLASKGVNAFSFMPYNAGGDGDNVWPF
                     VERNDKFHYDCSKLDQWGIVFDHATSLGLYLHFKLQEQEMDDNRLGEKANKKVVPESL
                     DGGVLGPERKLYCRELIARFAHNLALNWNIGEENTQSAEEQRAMIQYIWEMDPYKHHI
                     VIHTFPNWQDRVYSELLGDKSLLTGASLQNGWREVHQRTLQWVQASAKAGRPWVVAND
                     EQGPADLGVPPDPGYQGFDGVARSGPEDTGYTLHDIRKYTLWGNLMAGGAGVEYYFGY
                     KLPQNDLTCEDYRSRDRSWDYCRIALEFFSRFNIPFWNMTNANALVGNEKNDNTRYCL
                     AAPGEIYVVYLPTGGTCELDLSKASGTFSVRWYDPRRGGDLQEGSVKEVSAGKKVGLG
                     RPPAEENEDWVILVRK"
BASE COUNT         1396 a         1552 c         1768 g         1391 t
ORIGIN      
        1 cgaaagagag gtattctggc gggcgtgctc accgttgaag ttattctggc cgaattgtca
       61 ctcgacgtcc aatggaaaag cttgttaacc gacggggcga gtctcgaaaa aggaacggtg
      121 atcggccgat tacaggggcg cgtccgcgac atcctttcgg tggaacgcat ccttctcaac
      181 tttgtgggac gactgtcggg gatcgccacg ctgacacatt catacgtctc tgcggttcgg
      241 gggacaaaag cacggatcta cgatacgcga aagacgaccc cagggtggcg aagactggaa
      301 aaatatgccg tgcgatgtgg tggtgggcac aaccatcggt tgggattgga tcgcgcgatc
      361 ttgattaagg acaaccattt ggcatggtta gctgaagagt tgcaatgttc cctagaagat
      421 gccgcaaaga tagcgatccg ccgggccaga gagctttacc ccggccatcc gatcccctcg
      481 ggccaggacc cagccgacat cattgtggaa atcgaggtgg attccctgcc gcaactcgag
      541 actgtactct gcgagaaccc cgacattgtg cttttggata acatgtcgct ggaagaactc
      601 cgacaggcag tcctgttgcg gaaccagctt gcacctcatg tggaactgga ggcatccggt
      661 ggcgttaccc ttgaaaacgt tcgtgccgtg gccgaaaccg gtgttgaccg catcagtgtg
      721 ggggcattga cccatgcggc gccttggcta gacatcggtc tcgattgggg ggcggatggg
      781 tcaggcgccg tggagtgacc acggcctttc ctgtcgcaaa agaaatggcc cgacgactgt
      841 cgggccactc ttcagattgg gagggttcta gggatttggg gggccgagtt gttgggccct
      901 cagcctgtag tctcctcttc caattctcct gggagcgtat tggccttttc aatcagtgca
      961 tccgcccgcg acttgatttg agctttgtcg ccgccttgtc ggcacaattc aagaagttcc
     1021 tcggtgccct tgacaatctc cctataagtc gcctcaaact ctcccatggg aacatcttcg
     1081 tattgggcaa gtcccgcccg catgttttgc aaaagaccca cggcttcgtc tttcgttaag
     1141 ttgggtacct ttttaagggt acggatcgtg agattcttga tgtactgagc atgcgaagct
     1201 gggttcctgg agtcatcctt cttgggcggt ggcccaccgc tcccgcagcc tgttgcccaa
     1261 aagatggctc cggccaaaag aaagctaatc acaattctgc ttgcgttttg aaccgacttc
     1321 atacttacct cacgcttttg ctcaattgca ccaaatcgtg ccaaaccgtc acgacaaata
     1381 acgctccacc tcaaggcaac gtgactgctt cattgcctcg agagctgagc agtgcccccc
     1441 aagttgtcac gtcgatggtc tcttgaataa accgcacgga tccgtcagcc atgccaacat
     1501 tgacaccccc aggatggtaa ctacgggcac caattcgagc atcccacgcg tttcccggag
     1561 tcgcgcaacg gagtcgccga tcgttattgg gcgccttgca gtattgttcg ataagatctc
     1621 ggtaattgtc gtccaaggcg tttgcgtttg gggtcaacgt gcccttgggc gtcgaaccac
     1681 cactgatgcc accgccgaag gcaacacctg agacatggcc ccacgcaccg cgtgcgtccg
     1741 cggtttcagg agatgtacaa acctcggaaa ttgctacggt gttggaggaa ccatcaagga
     1801 tggattcaat ctttgccccc cactggaccc aagtgttgaa cacaccgcga tattccaacg
     1861 tggtgtaacc atctagatct gacatgtaac gacgtgcact acagttcgcg acatagttcc
     1921 ctttggcaaa ctcttgaccc gttgcacgga taaagacgcg gttatccgag gcatctgacg
     1981 ggcatattaa ggtagcgatt tgcgtcttga caaccggttg attgaccgga tcgatgcatg
     2041 ggcggcgaaa atcataattg gagtggagcg cttgctgttc caattgtggt aacaacattg
     2101 tgatccatgt cgcgccccaa gtatcgagtg tattccgcgg ttgtttgccc gccatcgcag
     2161 ctgtatccag cgccctataa gggaaacctg ctcccgtgca gcccagcgca gccgggggga
     2221 actgcttgtt aatatcgtgg taattgtgga gcgccaggac aacctgtttc atgttattgg
     2281 tgcattgatt tcgcctggct gcctcgcgtg ccgcctgaac cgctggaagg agaagcgcga
     2341 tcaaaatgcc gatgatagca atcaccacca gaagctccac caacgtgaag ccacgactcc
     2401 accggacaac aagtgccttt ttcatggcat cgcctccaca aaagaccaaa aacgaacacc
     2461 aggaggaggt caaaactgca ccatctcatc accgtcacca ttaaaacacg actacccgtt
     2521 gctgtccagg caaaaatacg tattttttct tagattatgc gtcatttcac gcttgtttta
     2581 accatctgtc ttcggacaaa ctggctggct acaatgggca agcgttggtg gcctaagggc
     2641 gacagaaaag ctttatgaaa cgagaccgat gagcgcgcaa cctcaccatg gcaaaaaaga
     2701 acagagctaa gcgaagggag ggggcgagcg tgccccgatt tgaacacgat cggttgccgc
     2761 ggattgccct cctaatcgaa acgtcgaatg aatatggacg tcgcttgctt ttaggaattc
     2821 gggattacgt gcgggagcat gagccatggt catttcaact tggggaacag gcccggggac
     2881 acacggtgcc gtcttggctt tcgaaatgga aaggcgatgg gatcattgcc aggatcgata
     2941 atgcctcgat cgaacaagca gtgcgcgaag tgggtgtccc ggttgttaat gtaagtgctt
     3001 cgggctttgg gttagcgttt ccaacggtca tcagtgatag tgaggccatt gcccaaaccg
     3061 cggcggacca ccttttggag tgtggttttt cccaattcgg gtattgtggc gatccccggt
     3121 tcgcttggtc taagagccat gaaaggcact tcaagcagta tcttcaacag cgagggtttt
     3181 cgtgtcattg tttttctggt tccaagtcag cggctaacca ctggggacgt gccctggaag
     3241 aactcatcgg ttgggtccgg gaactgccaa agccaatcgg gatcatggct tgttatgatt
     3301 tgcgtggtca gcaactgcta gcgacgtgtc gtgaggccgg aatccacgtc cccgatgagg
     3361 tggcagtcat cggacagcac aatgatgaga caatctgcga gttctgcgac ccgccgcttt
     3421 caagcgtgat cccgaaccca cggttggccg gttacttggg ggcatccctt ttggatcgga
     3481 tgctcaaagg ggaatggatt cccggagggg tttatcgagt tccaccgatc ggcatagcgg
     3541 tgaggcgttc cacggacgtc gtggccgtgc cggacgagga tgtatcggct gccattcggt
     3601 acatccggga ccacgcatgc caaggaatag acgtccgcga tgtcgttcac gcggtgaata
     3661 tttcgcgaag catgttagaa cgaaagttca agcggtacct caattgtaat ccccatgatc
     3721 taattgttcg cacacaattg cggcgtgccc aggagttgct gattcaaaca gacctgacga
     3781 tagaaaacgt ggccacgctg agcggtttct cgtcggctga gtatatgagt tgtgtctttc
     3841 atcggcgtgt tggccagtcg ccgcgcgact ttcgcaagcg gtgtcgaggc aagttgctct
     3901 gacaaaagag gggtgcaacc gcacagccgc cgagtgtgaa aaatcgaaga aaatttgtgc
     3961 tttttcacat ggtgacaacc ggttctgcgg attacactgg ttcttaggtc attcggcacg
     4021 aaaactaacc gtgcgatgtg atgtctttcg gtgagtgcga gtacaacggt gtgccggctt
     4081 tgataggctg aggactaagc ttaaggagcg aggatcatga caatgagaag actggtcgct
     4141 gtttgggttg ggcttttcct cgcagtagtt cttccgttgg taaggcaggt ccccagagca
     4201 cttggcgaaa gcggtggtca tggggacggt cgcgtgacca tttcgggaga gttgaagcgt
     4261 tggcacaagg tgacgctgac tttggcaggg ccatttgccc gtgaacgaga tgagagcccg
     4321 aatccgttta cagattacga ctttacggtg gaattccgac atgagtccgg cgagcccgtg
     4381 tacagagttc ccggttattt cgcggcggat ggtcaagcgg cggaaacgtc cgcggaagcc
     4441 ggcaactgtt ggcgagccca tctcgccccg gataaaacag gtgtctggag gtaccgggta
     4501 tcatttctca aaggcaaaca cgcggccatc gggggaaaag ggcaaccttt ggccccttat
     4561 cacggagtaa gtgggcaatt cacggtcgct gagaccgaca agacaggccg tgatttccgt
     4621 gccaaaggcc gtctggcata tgtgggaaag catcacctgc aattcctggg gacgggcgaa
     4681 ttctttttga aggcgggcgc ggatgccccc gagacattcc tggcatacgc ggatttcgac
     4741 ggaaccattg ccaccaagaa aaatgtgccc cttcacaagt gggaacccca catccgcgac
     4801 tggcaaccca gtgatccctc gtggaagaac gggaaaggta aagggatgat tggagcactc
     4861 aactatttgg cgtcgaaggg tgtcaatgcc ttttcgttca tgccttacaa tgccggaggc
     4921 gatggcgaca acgtttggcc gtttgtggaa cgcaacgaca agtttcacta cgattgctcg
     4981 aagttggatc agtggggaat tgttttcgac cacgcaacat cactgggact ttacctgcac
     5041 tttaagctgc aagaacaaga gatggacgat aatcggctcg gtgagaaggc taacaagaag
     5101 gttgtcccgg aatcgctcga tggcggggta ttgggacccg aacggaagct ttattgccgc
     5161 gaactgatcg cgcggtttgc ccacaatctg gcgctgaact ggaatatcgg cgaggaaaat
     5221 acgcagtccg cggaagaaca acgggcgatg atccaatata tttgggagat ggacccgtac
     5281 aagcaccaca tcgtgattca taccttcccc aattggcagg atcgtgttta ttccgagctc
     5341 ttaggggata agtcgctcct tacgggcgca tcattgcaaa acggttggcg ggaggtccat
     5401 cagcggacgc ttcaatgggt tcaggcctcg gccaaagcag ggcgtccgtg ggtagttgct
     5461 aacgacgagc agggaccggc cgatctgggt gtaccacccg atcccggcta ccagggtttt
     5521 gatggcgtgg cgcgttctgg accggaggat acgggttata cactccatga cattcggaaa
     5581 tacacattgt ggggcaatct gatggctggg ggggccggtg tggagtacta tttcggctac
     5641 aaacttcccc agaacgattt gacctgcgaa gattaccgaa gtcgcgaccg ctcttgggat
     5701 tattgccgca ttgcccttga gttcttttca cggtttaata tcccgttctg gaacatgaca
     5761 aatgccaacg cactggtggg aaatgagaaa aacgataaca cgcgttattg cttggctgct
     5821 ccgggcgaaa tctacgtggt ctatttgccc accggcggaa cctgtgagct ggatctctcc
     5881 aaggcatcgg gaacgttttc ggtgaggtgg tacgatccgc gccgcggtgg tgaccttcag
     5941 gaagggagtg tgaaagaggt gtcggcgggc aagaaggtcg gactggggcg tccgccggca
     6001 gaagaaaacg aagattgggt catccttgtt cgaaagtgac aagcatggaa accacttttc
     6061 gcatagccgt actaccaggg gatggtattg gcccggaggt gattcgg
//
LOCUS       DAOPTR010000075         5997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_10791_length_5997_cov_3.370077, whole genome shotgun sequence.
ACCESSION   DAOPTR010000075 DAOPTR010000000
VERSION     DAOPTR010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10791_length_5997_cov_3.370077"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PKI05_02490"
     CDS             <1..211
                     /locus_tag="PKI05_02490"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA51095.1"
                     /translation="GDFIECVKTREKPFRDIELAVNTMVVCHLGIIAYTLKRSLKWDA
                     AKQQFVNDPEADRLRDRARREPWVL"
     gene            279..980
                     /locus_tag="PKI05_02495"
     CDS             279..980
                     /locus_tag="PKI05_02495"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOA51096.1"
                     /translation="MLEDAFEALKTFDWGADPKVLKPIDDAIVATYGDAAGRRELENK
                     LLELLKSDVSRAAKDFICRKLRVIGTAASVPVLAGMLTDPENAHMARYALERMECPEA
                     AQALRDALPKVNGALKIGVIGSLGARQDAASVPALAALMTDSNPGVARAAVLALGNIR
                     TAEAAQALTAASVAPEVRPAVADAMLACAEALLAAGNKVQAMALYKKLMSDDQPKHVR
                     LAATRGMLVATGKSE"
     gene            1120..2532
                     /locus_tag="PKI05_02500"
     CDS             1120..2532
                     /locus_tag="PKI05_02500"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOA51097.1"
                     /translation="MLRTTAGLCVSLFFLFACGLGCSVLCLGEEASPPDTQLLQMVLE
                     LLREEDKDIRGLALEQIRNELKGEAVTRRLAEELPKLKSEAQVGLLGALVDRGDKAAR
                     PAIVSLLKETPDSAVRQAAMRALAFLGEPADAQLLLPFFLEGTDAERQAAQFALTRIP
                     GAEVGAAIIQAAERASAEQFRRLIALLLERRESAATARLLEWAGSGDPARRAAAVAAL
                     GDLAGPEQLPQMVRLVLATPPGRDREAVEKAVMLVCARIPDPEQRDASLLAAMKSLNE
                     TERVTLLPTLGRVGGKSALAMIEAAIQSQDAARHEAGIRAIANWPDGSVAERLIELAQ
                     HDPHPAHRITCLRALIRVAPLATDRSDEEKLSLLKTAMSLCTRDEERLLVLQRAQAIR
                     HVATLRYLMTFLDQPAYAEAACLSIVELAHHRDLREPNKAEFDKALDAVIRTSKDAVV
                     VERANRYKKGQTWVRPKGSQ"
     gene            3053..4624
                     /gene="purH"
                     /locus_tag="PKI05_02505"
     CDS             3053..4624
                     /gene="purH"
                     /locus_tag="PKI05_02505"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOA51098.1"
                     /translation="MSMIVKPHRALISVSDKLGLADFARSLRDFGIEIFSTGGTRRFL
                     QEQGIPVTDVAAYTGFPEMMEGRVKTLHPKVFGGILARRDHADDVRALEEHHILTFDV
                     VVVNLYPFRQTVARPDCTEADAIENIDIGGPSLVRAAAKNHAFVAIVTSPAQYELVLK
                     ELREHGGTTLALRRQLAAAAFAHTAEYDQAIATWFAQRVEEGPFPSRLFFTFERWTPL
                     RYGENPHQQAAAYRMPEVHGPSVVTARPLHGKELSYNNLLDLDSALEIVRHFREPAVA
                     VIKHNNPCGAAVAATLAEATRNALAGDPVSAFGSILGMNRTVDAETAEVLSEPGLFIE
                     AIIAPHFDAKAFEILTSKPKWKKNVRLLEVGDLTEERERWAYRHLTGGLLVQEADNLA
                     DDESQWQVVTQVRPDESLLPDLRFAWAMVRFVKSNAIVLARNGMLVGVGAGQMSRVDS
                     VEIAIRKAQDRAQGSVLASDAFFPFPDSIEHAAQAGIRAIIQPGGSVRDEEVIAACNK
                     HGLPMIFTGRRHFKH"
     gene            complement(4755..5402)
                     /gene="lexA"
                     /locus_tag="PKI05_02510"
     CDS             complement(4755..5402)
                     /gene="lexA"
                     /locus_tag="PKI05_02510"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOA51099.1"
                     /translation="MGKNDISAALSSLTPRQREILDFIRDWIEQFGYAPSVRDIGRHF
                     GIRSPNGVRGHLKALEAKGVIERDPRRSRALRLTPAARGRVSRSGKGFTLPLVGRIAA
                     GQLHEAIENAETFDFQSLFSSKSADHFVLRVHGDSMIEAQIADGDYVIVRRQETATPG
                     SIVVALTDENEATLKYWYPTKRGAKLVPANKRMAPIFVKNAKVLGVVVGVVRKLA"
     gene            complement(5528..>5997)
                     /locus_tag="PKI05_02515"
     CDS             complement(5528..>5997)
                     /locus_tag="PKI05_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51100.1"
                     /translation="VQANDGATYGYLDPGARPGTTAVGLLCRMYLGWDKNHPGLKKGI
                     AQLSAWGPSTDKSGPSKNNFYYNYYATQVLRHWEGEEWKKWNSVMRDYLVNTQAQKGH
                     ETGSWFFEDSSDHGKERGGRLYFTAMATLILEVYYLHMPLYRKQATAEDFPLD"
BASE COUNT         1198 a         1651 c         1861 g         1287 t
ORIGIN      
        1 tggcgatttt atcgaatgcg tgaagacgcg ggaaaagccg ttccgggaca tcgaactggc
       61 ggtcaatacg atggtcgtct gccacctggg gattattgct tacacgctta agcgatccct
      121 caagtgggat gccgcaaaac agcaattcgt caatgatcct gaggctgacc gactccgcga
      181 tcgtgcccgt cgtgagcctt gggttttgta attggaacat cggggatcag tgtgtgatat
      241 gagttaccag gaaatccatg aatttgcgag gatatgccat gttagaagac gcctttgaag
      301 ccctgaagac gtttgactgg ggggctgacc ctaaagtgtt gaagccgatt gatgatgcca
      361 tcgtggccac gtacggcgat gctgcggggc ggcgagagtt ggagaataag ctgcttgagc
      421 ttcttaagag tgatgtctcc cgtgctgcta aggactttat ctgtcggaaa ctgagggtaa
      481 ttgggacggc cgcaagtgtg ccagtgttgg ccgggatgtt gaccgatccc gagaacgcgc
      541 acatggcccg ttatgccctg gaacgaatgg agtgtcctga ggcggcccag gccctgcggg
      601 atgccctgcc caaggtaaat ggcgcgctaa agatcggtgt gatcggatcg ctgggcgcga
      661 ggcaagatgc ggcctcggtg ccagcgctgg cggccctgat gacggatagc aacccaggcg
      721 tcgcccgggc agcggtcctc gctttgggca atatcaggac tgcggaggcg gcccaggcgt
      781 tgacggcagc ctcggtggcc ccggaggtcc gacctgctgt tgcagatgcc atgctggcct
      841 gtgcagaggc actcttggcc gcgggcaata aggttcaggc catggcccta tacaagaagc
      901 tgatgagcga cgaccagccg aaacacgtcc gcttggctgc cacacgaggc atgctcgtgg
      961 cgaccggtaa gtccgagtag tcattaaacg cttgtagtgt ttgctagaga tgcggatgcg
     1021 gcagttccca tcccacggga ttgaggatta cgtcgcggtg gggtgaaaat ctgccgcatc
     1081 ccttcctgta ttctcgtggt tcattgggag accctagtaa tgttgcgcac taccgccggt
     1141 ttatgcgttt ccctgttttt cctcttcgcc tgtgggctgg gttgttcggt attgtgcttg
     1201 ggcgaggaag cctctccgcc ggatacccaa ctcctgcaga tggtcttgga gcttctgcgg
     1261 gaagaggaca aggacattcg cggattggcc ctcgagcaga ttcgcaacga actgaagggc
     1321 gaggccgtta ctcgacggct ggccgaggag ctaccgaaac tgaagagtga agcccaggtc
     1381 gggctgcttg gcgccctggt ggatcgcggt gacaaagcgg cacgaccggc gattgtgtcg
     1441 ctcctcaagg aaaccccaga tagtgccgtc cgtcaggccg cgatgcgggc actggccttt
     1501 cttggcgaac ctgcggatgc tcaactgctg ttgccgtttt tcctggaggg aaccgatgcc
     1561 gaacggcagg ctgctcagtt tgctctgacg cggattcccg gcgcggaggt aggagctgcc
     1621 atcatccagg ccgctgagag ggcgtcggct gagcagtttc gccggttgat tgcgttgctc
     1681 ctggaacgtc gggaaagcgc cgctactgct cgacttttgg aatgggctgg ctccggcgat
     1741 cctgcacggc gggccgcggc ggttgcggca ctgggcgact tggccggtcc cgagcaattg
     1801 ccgcagatgg tccgcctcgt gcttgctact ccaccggggc gcgaccgcga ggcggtggag
     1861 aaggctgtga tgctggtctg tgcgcgcatc cccgatcccg agcagcgtga cgcctccctt
     1921 ttggcggcca tgaagtcgct caatgagacc gagcgagtga ccctcttgcc gacgttggga
     1981 cgtgtcggcg gaaagtctgc cttagcaatg atcgaggccg ccatccaatc ccaggatgcc
     2041 gcgcgacacg aggcaggcat tcgggctatc gccaactggc cggacggctc ggtggccgaa
     2101 cgtttgatcg agttggcgca acacgaccct catcccgccc atcggatcac gtgtctgcgg
     2161 gcactcatcc gtgttgcgcc cttggccacc gatcgtagcg acgaggaaaa actgtcgctg
     2221 ctcaagaccg ccatgagtct ttgtacacgt gatgaggaac ggctcctcgt tctccagcgg
     2281 gcgcaggcga tacgtcacgt ggccacgctg cgatacttaa tgacgtttct cgatcagcca
     2341 gcgtatgcgg aggcggcctg cctatcgatt gtggaactgg ctcatcaccg agacctacgt
     2401 gagcccaata aagccgagtt tgacaaggcc ctggatgcgg tcatccgcac gagtaaagac
     2461 gccgtcgtgg tggaacgagc caatcggtac aaaaagggcc aaacctgggt ccggccgaaa
     2521 gggtcgcagt aaactgcctc ggacgccgtt ccagcacaac tccggcagcg aggtagatcc
     2581 ggcagcggaa taaaaggaga agcccattag gaaaccgggg cgggggtatg ttgttgcgca
     2641 cgtgatgcgc tttcactttg aaactcctca gaaaatgaca ggggctttcg ctcgttgaga
     2701 ggcccctcac agcttagggc ctatcccaaa gttccctctc cctctgggag agggttgggg
     2761 tgagggcaag cgatcgtcgg agtgggtcgg aaaaaccggg agcgattcga gcgaccctca
     2821 cccccggccc ctctgccccc gaacgggaga ggaaaggctg tgggataggc tcttaaccaa
     2881 acatgcgggg ataccgatcc tcgcgggcaa agttccatag cgactgaggc cggcggggac
     2941 ccgaaccaac ctcatgagat cggccttcgt tccaccttcg gccaatagac cttaaaataa
     3001 ggcttttacc atctcgcttg attgggtcgt gttttcatcg cgtctggagt gaatgtccat
     3061 gattgtcaaa ccgcaccggg cgttaattag cgtgagtgat aagttgggtt tggccgattt
     3121 tgctcgaagc ctacgcgatt ttggaatcga gattttcagc actgggggta cgcgacgctt
     3181 cctacaggag caggggatcc ccgtcacgga tgtggccgct tacacgggct ttcccgagat
     3241 gatggagggg cgcgtcaaga cactccatcc caaggtcttt ggaggaatcc ttgcacggcg
     3301 ggatcatgcg gatgatgtgc gggcactgga ggagcatcat atcttgacgt tcgacgtggt
     3361 ggtggtgaat ctctatccgt ttcggcaaac ggtggcccgc cctgactgca ctgaggcaga
     3421 tgctattgaa aacattgaca tcgggggacc atcccttgtc cgtgcggctg ccaagaatca
     3481 tgcctttgtg gccatcgtca caagtcctgc ccaatatgaa ctggtgctta aagaactccg
     3541 tgaacacggc gggactacct tggctttgcg gcggcagttg gcggcggcgg ctttcgccca
     3601 tacggcggaa tacgatcagg ccattgccac ttggtttgcc caacgtgtgg aagaagggcc
     3661 ttttccctcg cggctgtttt tcacatttga gcggtggacg cctctccgct atggagaaaa
     3721 tcctcaccaa caagccgcgg cgtatcggat gcccgaggtt catggtcctt cggtggtgac
     3781 ggcccggccg ttgcatggaa aagaactttc ctacaacaat ttgcttgacc ttgattcagc
     3841 cctggagatt gtgcgccact ttcgggaacc cgcggtggcc gtgatcaagc acaacaaccc
     3901 ttgtggggcg gccgtggcgg ccacgttggc ggaggccaca cgaaacgcac tggccggcga
     3961 ccccgtcagc gcgtttggtt cgatcctggg gatgaatcgc acggtggacg cggaaacggc
     4021 cgaggtcctt tctgaacccg gcttgtttat tgaagcgatc attgccccgc attttgatgc
     4081 caaggccttt gagattctga ccagcaaacc caagtggaag aaaaatgtcc gcctgctgga
     4141 ggtgggggac ctgacggagg aacgcgaacg atgggcgtat cggcatctga cgggcggtct
     4201 tctcgttcag gaggccgata atttagccga cgacgaaagc caatggcagg tcgtgacaca
     4261 agttcggccg gatgaaagcc ttctccccga cctgcgcttt gcctgggcca tggtgcgatt
     4321 tgtcaagtcg aacgccattg tccttgctcg gaatggaatg ctcgtcgggg taggagcggg
     4381 acaaatgagt cgggtcgact ctgtagaaat tgccatccgc aaggcgcaag accgggccca
     4441 gggaagcgtc ctagcgtcgg atgccttttt ccccttccct gattccattg agcacgccgc
     4501 gcaggccggc atccgcgcga ttatccagcc cggagggtcc gtgagagatg aggaggtcat
     4561 tgccgcctgt aacaagcatg ggttgccgat gatcttcacc ggacggcggc actttaagca
     4621 ttgatactaa ctgatgcggc cgtcccatcg cggcgcggtg cgataacaca cgacgggcga
     4681 caatgttgtc gccgcaattg gcgtcataac gaggctagaa cgacacccgt ctcaatcatg
     4741 cggcggaagg ggtgttacgc gagttttctg accacgccca cgacaacccc caggaccttg
     4801 gcgtttttga cgaagatggg ggccatccgc ttgttggcgg ggacgagctt ggctccccgt
     4861 tttgtgggat accagtactt gagcgtagct tcgttttcat cggtcaaagc cacgacgatg
     4921 ctgccgggtg tggccgtttc ctgccgtcgc acgatgacat agtcaccgtc ggcgatttgg
     4981 gcctcgatca tggaatcgcc atgaacacgg agcacgaaat gatcagccga cttgctgctg
     5041 aaaaggcttt ggaaatcaaa ggtttcggca ttttcaatag cttcgtgaag ttggccggcg
     5101 gcaatgcgcc ctacgagcgg cagcgtaaac cccttaccag atcgtgagac cctgccacga
     5161 gcggcaggtg tcagacgcaa ggccctggac cgccgcggat cgcgttcgat gacacctttc
     5221 gcttccaggg ccttcaagtg tccccgcacg ccattgggtg agcggatccc gaaatgacgc
     5281 ccgatgtccc gtaccgatgg ggcgtaaccg aactgctcga tccagtcccg aataaaatcg
     5341 agaatctctc gctgtcttgg cgtcaatgac gaaagggcgg cggatatatc attttttccc
     5401 atggctcatt cgctcgtttt aaaaaagccg agtgcccagg ctggcattgt gtacaagtgt
     5461 acaaccaacc cgtcggtgtg tcaagagccg gcaagccatt gaccaccgat ctgactgaac
     5521 cgtgtcccta atcgagcggg aagtcttctg ccgttgcctg tttgcgatac aggggcatgt
     5581 ggagatagta aacctcgagg atgagcgtgg ccatcgccgt gaagtagaga cggccaccgc
     5641 gttctttgcc gtggtcagaa ctgtcctcaa agaaccagct tcccgtttcg tggcctttct
     5701 gcgcctgcgt gttgacgaga taatcgcgca tgacgctgtt ccactttttc cattcttcgc
     5761 cttcccagtg ccgcaagacc tgagttgcgt agtagttgta gtagaagttg ttcttactcg
     5821 gtccgctttt gtcggtcgat ggcccccatg cactgagctg tgcgatgcct tttttcagtc
     5881 cggggtgatt cttgtcccag ccaaggtaca tccgacagag gaggccgacg gcggtcgtac
     5941 ctggacgggc accagggtcc aaatatccgt aggtggcccc atcgttggct tggacag
//
LOCUS       DAOPTR010000076         5992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_10808_length_5992_cov_3.060131, whole genome shotgun sequence.
ACCESSION   DAOPTR010000076 DAOPTR010000000
VERSION     DAOPTR010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10808_length_5992_cov_3.060131"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PKI05_02520"
     CDS             complement(<1..226)
                     /locus_tag="PKI05_02520"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOA51101.1"
                     /translation="MDNLLGDNLRRIMSQLGLTINEVAERTKLDKRTIRGILEGSKRP
                     HPKTIGQLAEGLGVSVDELYIDPTRLLYRRL"
     gene            599..1168
                     /locus_tag="PKI05_02525"
     CDS             599..1168
                     /locus_tag="PKI05_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51102.1"
                     /translation="MVAKIEIPCPCGKSVPVTINQCGMIVTCSCGRELAVPSRRELLG
                     TRQTIDEDASVEEEKAPLSGWVRVFQWISGSVAVLALIGLVVLYIYRPRLPDFHKLPP
                     AVAYQYFKVLRTGERSPWLEMEARYVRDHNIWLGMRDIVAVVGIIAIVLFCGVTLAGW
                     WETCSNRKAEDEMGEPPPGAESEGTESPS"
     gene            complement(1180..2961)
                     /locus_tag="PKI05_02530"
     CDS             complement(1180..2961)
                     /locus_tag="PKI05_02530"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA51103.1"
                     /translation="MQQRSFVNIFGLIFLPWAIGCLLASVVGYVGLTWQERSLKQAQA
                     EEWERFISGLIEGEIGSQPKITDLQESRLLQSLIQNTRLELAVFTASGKYVVGSVTNP
                     PPNIPSEVQMVLKTSQRFTTWTEKRGQGRPQLRMAWPRQVEGKTHIIWAAIDLGTVTG
                     NGPSLRSLLAWNVVFFAIGLPFAWGLTWWLRRRLDVVTKSALEGEPSLRPWDLWEWHA
                     LAHRLRDYVAQQRQQIEVLQGIARDHQAVLEHLPDGLLFVTPAGRVAKHNPAAGQLLR
                     FPGTLAEGLSIRECLRKPEFLTWVENLLSLKRPPVLSLHNDDPEQHLEIRGAELKGPD
                     QRVHGFLLIIRDMTRTWAADRVRRDFVANVSHELRTPLTSIKGFLETLLDGALEDPPQ
                     ANRFVRIIHEQTERLERIVNDLLILARLEGEHEQPITREPVRVEELVLGAVDACRYQA
                     EQRGMQIHWEVPGDLTIRVNGRLLHQALVNLLDNAIKYSDPNKEIRIVVRRGEKELIF
                     EVQDQGWGIEARHLPRIFERFYRVDPSRSRQLGGTGLGLAIVKHVAQIHGGRVTVDSR
                     VGVGTTFRIHLPLEHVLHERPTGSQLE"
     gene            3223..4494
                     /locus_tag="PKI05_02535"
     CDS             3223..4494
                     /locus_tag="PKI05_02535"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOA51104.1"
                     /translation="MQPEQGAATPSFESESHRIPVPFLDLARSHRPLRDQIVEALARV
                     CDSGAFVLGPEVEHLERILAQYCHCEHAIACASGSDALLLALMALGIGPGDEVIVPSF
                     TFFATASAVTRLGAKPVFADIDPATYNISPREVARLIHKNTKAIIPVHLFGQAAEMEP
                     ILRLAQQHHIFVIEDAAQSIGAEYHQRRVGSLGQIGCFSFYPTKNLGAMGDAGMITTH
                     DAELAEKLRVLRVHGMKPRYYHHWIGINSRMDAFHAATLLVKFNCLETWIAERQQIAD
                     RYYQLFAEYKLAQVILPQVLPGRRHVWNQFVIRIPHGQRDSLRAYLQERRIGTEIYYP
                     LGLHEQACFQDLGYAPSDLPATFAASREVLALPIFPGLTSDEQRTVVATIAEFFKNHP
                     PRIQVGQSYFLKHPATLRGMRQASVSKGEAD"
     gene            complement(4587..4871)
                     /locus_tag="PKI05_02540"
     CDS             complement(4587..4871)
                     /locus_tag="PKI05_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51105.1"
                     /translation="MTLSGIDLLSFVVLAQEGTSATSWAPGYLVTILVLLFVMMVTLA
                     PSRRADKPKQFEFTGTSWGKVVTGETEKKQEPQGPPPGVFAPGQLAQKRK"
     gene            complement(4957..5733)
                     /gene="pstB"
                     /locus_tag="PKI05_02545"
     CDS             complement(4957..5733)
                     /gene="pstB"
                     /locus_tag="PKI05_02545"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOA51106.1"
                     /translation="MHAEFAQETPILEIDHFSLSYGPKQVLFNISMIIPRGKVTAIIG
                     PSGCGKSTLIRCINRMNDLIESVRIEGDIRLDGTSVFDPAVDVTEIRKRAGMVFQKPN
                     PFPMSIFENVVYPLRIQGVRDRRVLAEVCERSLRAAALWDEVKDNLHQNAMQLSGGQQ
                     QRLCIARALAGDPEVLLLDEPCSALDPIATNKIEDLIRELAGTYTIVIVTHNMQQAAR
                     ISDFTALLYLGRLIEYGPTRRIFSAPHLPETEAYVTGRFG"
BASE COUNT         1297 a         1660 c         1654 g         1381 t
ORIGIN      
        1 ccaaccttcg atagaggagg cgagtgggat cgatgtacag ctcatcgacc gagaccccca
       61 atccttcggc gagttggcca atcgtcttgg gatgaggtcg cttactccct tccagaatgc
      121 cccgaattgt ccgcttatcg agttttgtcc gttcggcgac ctcattgata gttaggccga
      181 gttgagacat gatccgtcgc aggttatcac caagcaggtt gtccatgatg cgttactccc
      241 tccgccgagg agcccacggt cttcatgggt taccccctgc ctcagatgtt gccgacatcg
      301 gggaaaaggc caacccggtt cgtcctacgt aataaccaac gttaagttgt ggtgcggttt
      361 tcgcaatcga ggcatgaaac agtggttgac aaaggagttc aaggccttcg agaaccgaca
      421 tagaaaaatt ttaacagacg taagtgctgc cttgctatgc cataggacgc cgctctctat
      481 gattaacttt acgccgatct gtatctcagg tacagcgtcc gacagcaatt tatgctaaaa
      541 aaggtagggt ggtcttccag ccgggccatg catggtcaaa cactcgcagg ttgttcttat
      601 ggtagcaaag attgaaatcc cctgcccatg cggcaaatcg gtacccgtga caatcaacca
      661 gtgcggaatg atcgtcacct gttcatgtgg ccgcgaactt gcggtccctt cacgccgaga
      721 attgcttggg acgcgacaga ccattgatga ggacgcctca gttgaagagg aaaaagcccc
      781 tctttcaggg tgggttcgcg tatttcagtg gataagcggc tccgtggccg tgcttgcact
      841 tatcgggctg gttgtgctgt atatctatcg cccacgactg ccggactttc acaaactccc
      901 gccggccgtg gcgtatcagt attttaaggt cttgcgcacg ggtgagcggt ctccgtggct
      961 ggagatggag gcgagatacg tccgcgacca caacatttgg ctggggatgc gggatattgt
     1021 ggcggttgtt ggaattattg cgatcgttct cttttgcggc gtcactttag cgggttggtg
     1081 ggaaacctgt agcaaccgta aagcagagga cgagatggga gaaccacccc ccggggccga
     1141 atcggaggga accgagtcgc catcgtgacc cacgtcgctt cattccaatt gtgagcctgt
     1201 gggcctttcg tggaggacgt gctcgagagg caagtggatg cggaaggtgg tgcccacacc
     1261 gacacggctg tcaaccgtga cacgcccccc gtgaatttgg gcaacgtgtt tgacgatggc
     1321 cagtcctaag ccagtgcctc ccaattggcg actccggctt ggatccacgc gatagaacct
     1381 ctcaaagatt ctcggcaggt gtcgggcttc gataccccaa ccttggtctt gaacctcaaa
     1441 gataagctcc ttttcgccac ggcggacgac aattctgatt tctttattcg gatcgctata
     1501 tttgatggcg ttatcgagaa ggttgacaag ggcctgatgc aataatcgac cgttcacacg
     1561 gatcgttaag tcgccgggga cttcccagtg gatttgcatg ccgcgctgtt cggcctgata
     1621 ccggcaggca tcgaccgcac ccagaacgag ttcctcaacg cggacaggtt cccttgtgat
     1681 gggttgctcg tgttcacctt ctagtcgggc gagaatcaaa agatcattga cgatccgttc
     1741 aagacgttcg gtctgctcgt ggatgatcct tacgaatcga ttggcttggg gtggatcctc
     1801 aagggcgccg tccagtagtg tctcgagaaa ccctttgatg gaggtcagcg gggtgcgtag
     1861 ctcgtggctc acgttggcca caaagtcccg ccgcacacgg tccgctgccc aagtccgtgt
     1921 catgtcgcga atgatcaaaa ggaagccgtg aacgcgctgg tcagggcctt taagttcagc
     1981 tccccggatt tccaagtgtt gctcgggatc gtcattgtga agcgacagca caggggggcg
     2041 ctttagcgac aagagattct cgacccaggt caggaactcc ggtttacgca agcattctcg
     2101 gatagataga ccctcggcca gagtaccggg aaatcgcaac agttggcctg ctgccggatt
     2161 gtgcttagcg acacgacctg ccggcgtgac gaaaaggaga ccatctggga gatgttccag
     2221 gactgcttga tggtctcgtg caataccttg caaaacctcg atctgctggc gctgctgtgc
     2281 cacatagtca cggaggcggt gggcaagggc gtgccactcc cataagtccc atgggcggag
     2341 ggaaggttcg ccttctaggg cggattttgt aacgacgtcc aaccgtcgcc tcagccacca
     2401 tgtcagcccc cacgcaaagg ggagccctat cgcaaaaaag acaacattcc acgccaagag
     2461 actgcggagg gaaggaccat tgccggtcac cgttccgagg tcaatggccg cccaaatgat
     2521 gtgtgtctta ccctcgacct gtcttggcca cgccatgcgc aactgagggc gaccttgccc
     2581 gcgtttctct gtccaggtgg tgaaccgctg gctggttttt aagaccattt gaacttctga
     2641 cgggatgttg ggcggtggat tggtcacgct cccaaccaca tattttcccg aggccgtgaa
     2701 caccgcgagc tccaaacggg tgttctgaat cagggattgc aagagcctgc tctcttgtaa
     2761 gtcagtgatt ttgggctgcg agccgatctc cccttcgatg agaccgctta taaatcgctc
     2821 ccactcctcg gcctgggcct gtttcagtga tcgctcctgc cacgtaagac cgacgtagcc
     2881 aaccactgat gccagaagac agccaatcgc ccagggtagg aagatcaacc cgaagatgtt
     2941 cacaaaagat cgttgttgca tgggcggcgc gatcccctat aaaggtggtc tattgccatc
     3001 tgatagactt atcatcgtct gagcatcccg ggctattatg tgtaatgtcc tttccttgtc
     3061 cggctgagca gcagtgaagc cggacggcct tagggggaac ctctccactt tgggtaaaaa
     3121 atttgtcttg acgaacgcga ctccaaaact ctacgctaaa gcgaacgcac taaactgatc
     3181 atccagcaga aattgtgccg gcaaccaagg aagggtcctt ttatgcagcc agagcaggga
     3241 gcggcgacac cgagtttcga aagcgaatct catcgcattc cggttccatt cttggatctt
     3301 gctcgaagtc acagaccctt gcgagatcag atcgtcgagg ccttggcacg ggtttgcgat
     3361 tccggggcct ttgtgttggg acctgaggtc gaacacctgg agcggatcct cgcccaatat
     3421 tgccactgcg agcacgctat tgcctgtgcc tcggggagcg atgccctgct gcttgccctc
     3481 atggccttgg gaattgggcc aggcgacgag gtgatcgttc ccagttttac gttctttgcc
     3541 acagcgagtg cggtcacccg actgggcgca aaacccgtgt tcgcggatat cgatccggca
     3601 acctacaaca tctctccccg agaagttgcc cgactgatcc ataaaaacac caaggcgatt
     3661 attcctgttc acctttttgg ccaagctgcg gaaatggaac ccattttgag gctggcccaa
     3721 cagcatcaca tctttgtcat tgaggacgcg gcacagtcga ttggggctga gtaccatcaa
     3781 cggcgtgtgg gatctttggg ccagatcggc tgttttagct tttatcctac gaaaaacctg
     3841 ggagccatgg gcgacgccgg aatgatcacc acgcacgatg ccgagcttgc cgaaaagctg
     3901 cgggtcttac gggtccatgg catgaaacca cgatattatc atcactggat tggcatcaac
     3961 agccgcatgg atgcttttca cgcggccacg ctcttggtta agttcaattg cttggaaacg
     4021 tggattgccg aacgacagca gattgccgat cgctattatc agctctttgc cgaatacaaa
     4081 ctggcacagg tcattctgcc acaggtcctt cctggccgcc ggcacgtgtg gaatcagttt
     4141 gtgattcgca ttcctcatgg gcaacgggat tccctgcgag cgtacctcca ggagcgccgg
     4201 atcggaacgg aaatctatta tccgttaggg cttcacgaac aggcgtgttt ccaagattta
     4261 gggtatgccc cgagcgattt acctgctacc tttgctgctt cgcgggaagt gcttgccctg
     4321 ccgatcttcc ccggattgac aagcgatgaa caacgcaccg ttgtcgccac cattgcggag
     4381 ttctttaaaa atcatcctcc gcggattcag gtcggccagt cgtacttcct gaaacacccg
     4441 gccacgctgc gcgggatgcg acaagcttca gtttccaaag gcgaagcaga ctgaaaaccc
     4501 tcgtccattc tcgaacaaac acccgatcgg gataacgaga agttgtcggt gcctgggaca
     4561 acgttttcca ccccggaacc ggaaggttat ttccgctttt gtgccaattg tcccggagca
     4621 aaaacccccg ggggaggccc ttggggctcc tgcttcttct ccgtctcgcc ggtcaccacc
     4681 ttgccccagg atgttcccgt gaactcgaat tgtttgggct tgtcagcccg ccgactggga
     4741 gccagcgtga ccatcatgac gaacaaaagc actagaatcg taaccagata gcctggtgcc
     4801 cacgaagttg cgctggtacc ctcttgcgcc agcaccacga acgacaatag atcgatcccg
     4861 gataacgtca tatagtcaac tcctgaatgg gttattttcc gtgcacttct ccactcgatt
     4921 ttacatggtc cccgcattct tgaaaacaaa gcccgcttag ccaaaacgtc cggtgacgta
     4981 agcctcggtt tcaggcagat gcggggcact gaatatccgg cgcgttgggc cgtactcaat
     5041 gagccgtccg agatagagaa gggcggtgaa gtcgctgatc cgtgccgcct gttgcatgtt
     5101 gtgggtcaca atgacgatag tataagtccc tgccagttcc cgaatgaggt cttcgatctt
     5161 attcgttgca atcgggtcaa gcgcagagca cggttcatcg aggagaagga cctccggatc
     5221 tccggccagg gcccgcgcaa tacacaatcg ctgctgctgt cctccggaca gttgcattgc
     5281 gttttggtgg aggttgtcct tgacctcgtc ccaaagggcg gctgcccgca gactccgttc
     5341 gcagacttct gccaaaactc ttcgatcccg aacaccttgg atgcgtaacg gatagacgac
     5401 attttcgaaa atactcatcg ggaacgggtt cggtttttgg aacaccatcc ctgcccgttt
     5461 gcgaatctcg gtcacatcca ccgcggggtc gaagaccgaa gtcccatcca gccggatatc
     5521 cccctcgatg cgtaccgact cgatcagatc gttcattcgg ttaatacagc gaatgagcgt
     5581 cgattttcca cagccgctgg gaccaatgat tgcggtgacc tttccacgcg gaatgatcat
     5641 cgagatgttg aagaggacct gcttcgggcc gtaggagagg ctaaagtgat caatctctaa
     5701 aatcggcgtt tcttgggcga attcggcatg aatctcgctt tcaaagggtt cgccgcgggc
     5761 aatggcatcc aaccgggaag caccggccga gaggaccttt gccaacggaa caggcgtccc
     5821 taaagccaca ggggaggctg tttctgtttc cgtctgtatg agtttgcctg tacccggact
     5881 ctcatcgggc cactggtctg gcataagagt ctcccgacgc cgttttcggt gggtcacggc
     5941 cggtccatcc gcgatcaagc gggtaagggg gccgtttgcc gtcagctttt ct
//
LOCUS       DAOPTR010000077         5969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_10854_length_5969_cov_3.257186, whole genome shotgun sequence.
ACCESSION   DAOPTR010000077 DAOPTR010000000
VERSION     DAOPTR010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5969
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10854_length_5969_cov_3.257186"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="PKI05_02550"
     CDS             <1..1222
                     /locus_tag="PKI05_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOA51107.1"
                     /translation="GKWPQRTAITDYIGAPQPAGWNRNTRLLPAAYETALPLAEITLA
                     ELVKRRNYATFFAGKWHLGPEGFWPENQGYDINMGGIDRGGPYGGNKYFSPYGNPRLP
                     DGPPGEHLPDRLARETAKFIEEHKDQPFLAFLSFYSVHTPLMAREDLRQKYEEKRRRL
                     GLEAKFGREGPRDVRLVQEHAVYAGMVEAMDQAVGKVLQKIDELGLRDRTVVFFTSDN
                     GGLSTSEGWPTSNLPLRAGKGWIYEGGVRVPLIVRWPGVVPPGTVSRVPVCSVDFFPT
                     IGDLAGAPWNDIPVDGQSLVPLLKGKPFPEDRPLFWHYPHYGNQGGAPSAAVRKGPWK
                     LIHWYEDDSWELYDVVADIGETQNLAQKHPERVAELRQLLETWLKDVGARFPTANPNY
                     DPNKPDGRIAVRPQ"
     gene            1262..2020
                     /locus_tag="PKI05_02555"
     CDS             1262..2020
                     /locus_tag="PKI05_02555"
                     /inference="COORDINATES: protein motif:HMM:NF023410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase-associated EpsI family protein"
                     /protein_id="HOA51108.1"
                     /translation="MNDSLRKLLPAWLAKNGWFFLAVGVIIAAAIVEGHWSLRWGSHS
                     ERVMAPLAQAVMQTPLTVGEWVTDEAVQPDPRVLEISGAEALFQATYRESTQGDVVTV
                     YLLAGWSRDISIHTPDACYPGAGFVMETSPQPFAFSYEANSQAGEQSSTGLRQAEFST
                     AIFTKTEPTGVTRLRVFWSWNDGRGWQAPRFPRWAFGGRRPLVKLYLVSESPPGQPTH
                     ESPALRLASALLPVLDRQLETALSTRPKERMSVP"
     gene            complement(2080..3969)
                     /locus_tag="PKI05_02560"
     CDS             complement(2080..3969)
                     /locus_tag="PKI05_02560"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOA51109.1"
                     /translation="MRWKLLWTVIATILSVCQGAWGVCEDHVASPPNVVFILADDLGW
                     SDLRIYGSVYHETPNLDRLARRGLRFTQAYAANPLCSPTRSSILTGLYPARIGITSPA
                     CHLPQAVLEKGLVPPQPNVRIRTANSLTRLKPEYVTLAEVFREHGYMTAHFGKWHLGH
                     SGPYEPKEQGFMLDFPNTPAAPGPGGGYLAPWKFIKDPAIQGQPGEHIEDRMSQEAAR
                     FIEENKDKPFYLNYWCFSVHSPWSAKPELVEYFKTKAKDDSPQRNPVYAAMVKSLDDA
                     VGRIVEAIDKAGLAERTIIIFFSDNGGFAYPPKNETQPPGYAEIPATSNTPLRSGKAS
                     IYEGGTRVPLIIVWPGHTQPDSVTDVLFSSVDFFPTILEMCGLPLPGHKVDGVSQVPA
                     ILGKGPVRTRVFCHFPHGGENQAQQIPGFLPSAYVRDGDWKLIRFFAGNPDGTDRFEL
                     YNLREDIGETHDVAAEHPEIVGRLAKELEGFLQDTDAVIPQVNPDYRGPQTSDPLMGW
                     KNRGCEATIDAGLLKLQAQTATPFLGFGVGKQPGPVQVTFRIKVPQAGEGKIEWIPPG
                     ASQDAAQSVPYSVTGTEMTTVTVRVPWEKPIGILRIYLPTGANTAEVDWIELETASGK
                     RRFDF"
     gene            complement(4240..5715)
                     /locus_tag="PKI05_02565"
     CDS             complement(4240..5715)
                     /locus_tag="PKI05_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 140 protein"
                     /protein_id="HOA51110.1"
                     /translation="MVVVTLAILFSWPCGSVRLFAEEFQQLEPLKVSDNGRFLVTVSG
                     KPFFWLGDTCWHMFGKSVHEGQDNQPSVELYFANRAKKGFTVIQSVLVREPVGGSYRN
                     AYGFEPFIDTDWSRPLIKPGENDDYWDHVDWCVQAAKRHGLRLAILPMWLQSIPPGHP
                     METNPEVAYRYGYFLGKRYRREPHIIWVLGGDAYEKGRNVDNPSRLALVRAVAEGIAD
                     GIIGNDQFDGNANWDVALMTFHPPGGNRSPGQWLHNEPWLDFNMIQTTTRFRFQNWKT
                     VTADYQRQPPKPTLDAEVTYEDSLSLDRQEPQDRRIRQWDVRRAAYWSVFAGGFGFTY
                     GHRSFIGWIRKGETYRYGAYIPWYESLDAPGAFQVVHLRRLIESRPFLTRIPDQSLLT
                     DDLGDGDDHLQATRDELGRYAMVYSPMGGTVTVKTHSLAGPARRAWWFDPRTGQATPI
                     QGDFSGNELHFTPPTQGEGQDWVLVIDAAAENFPPPGSTSN"
BASE COUNT         1288 a         1629 c         1752 g         1300 t
ORIGIN      
        1 cggtaaatgg ccacaacgga cggccattac cgattacata ggggcccccc aaccagcggg
       61 ctggaaccga aacaccaggc ttcttccggc agcctatgaa acggctcttc cgcttgcgga
      121 gatcacgcta gccgagttgg tcaaacggcg gaattatgcg acgttctttg ccggaaaatg
      181 gcacctgggg ccggaagggt tttggccgga aaaccagggg tatgacatca acatgggcgg
      241 gattgaccgc ggcgggcctt acggcggcaa caagtacttt tctccttatg gaaatccccg
      301 cctgcccgat ggaccgcccg gcgagcatct tccggatcga ttggcgagag agacggccaa
      361 gttcatcgaa gagcataagg accaaccgtt tttggcgttt ttgagtttct attctgtcca
      421 tacgccattg atggcccggg aggacttacg gcaaaagtat gaggagaaac gccgtcggct
      481 gggactcgag gcgaaattcg gtcgagaagg gccgcgggat gtccgccttg tccaagaaca
      541 tgccgtttac gcggggatgg tcgaggcgat ggaccaagcg gtagggaagg tcctccaaaa
      601 aattgacgaa ttgggcctga gagatcgtac ggtggtgttt ttcaccagcg acaacggagg
      661 gctttcgacc agcgagggat ggcccacgtc gaatcttccc ctacgggccg ggaagggctg
      721 gatttacgaa ggcggcgtgc gggttccttt gattgtgcgt tggcccggtg ttgttccgcc
      781 tggcacggtc agtcgtgtcc ctgtgtgtag tgtggatttc tttcccacga ttggcgatct
      841 ggccggtgca ccatggaatg atatccctgt ggatgggcag agtcttgtgc ccttgctgaa
      901 gggaaagccg tttcccgaag atcgtccgtt gttctggcat tacccgcatt atggaaacca
      961 aggtggggcg cccagcgcgg ccgtgagaaa aggcccctgg aagctgattc attggtatga
     1021 agatgattcc tgggaactct acgatgtcgt tgccgatatt ggcgagacac aaaacctcgc
     1081 tcaaaagcac cctgagcgtg tggctgaact ccgtcagctt ttggagacgt ggttgaagga
     1141 tgttggggca aggtttccca ccgccaatcc caactatgat ccaaacaaac cggatgggag
     1201 gatcgcggtc cggccgcagt aaaaagtggg agcggtgcgc gtttggcagg aaatgtggct
     1261 tgtgaacgat tcgcttcgta aattattgcc ggcgtggttg gcaaagaacg gctggttctt
     1321 cctggccgtg ggggtgatca tcgcggcagc gatcgtcgaa ggccactggt cattgcgttg
     1381 gggttcccac agcgaacggg taatggcgcc attggcgcag gctgtcatgc aaacgccgct
     1441 gacggtggga gaatgggtta ccgacgaagc ggtgcagccg gacccccgcg tgctcgagat
     1501 cagtggcgcg gaagccttgt ttcaggccac ctatcgcgaa tccacccagg gtgatgttgt
     1561 gacggtgtac cttttagccg ggtggtcgcg agacatttcc atccacacgc ccgacgcttg
     1621 ttatccaggg gcgggctttg tgatggagac gtcccctcaa ccattcgctt tctcctatga
     1681 agcgaattcc caggcaggcg agcagtcctc cacgggcctt cggcaggccg agttttcaac
     1741 ggccatcttc accaagacag aaccaaccgg tgtgacgcgg ctgcgggtgt tttggtcgtg
     1801 gaatgatggg cgcggctggc aggccccccg atttccccgt tgggcttttg gaggccgacg
     1861 gcctttggtc aagctctatt tagtgagcga gtctccgcca gggcaaccca cgcacgaaag
     1921 tcccgccctg cgtcttgcct cggccctgtt gcctgttctc gatcgacaat tagaaacggc
     1981 attatcaacc cgccccaaag aacggatgtc agtgccttaa aaaatggcgc cagctaaata
     2041 ggttaagagt tgtcgccagc ataatctgcc ccggtctttt cagaaatcga accggcgctt
     2101 acccgacgcc gtctctagtt cgatccaatc gacctcggcg gtgttggccc ctgtggggag
     2161 gtaaatccgc aggatgccga tgggtttttc ccagggaacg cgaacggtga ccgttgtcat
     2221 ctctgtgcct gtgacactgt aggggacgga ctgggcggcg tcctgacttg ctcctggtgg
     2281 aatccactca atttttccct caccggcttg agggacctta attcggaaag ttacctggac
     2341 cggtccgggc tgcttgccga caccgaatcc caagaatggc gtggccgttt gggcctgcaa
     2401 cttaagcagg ccggcgtcga tcgtcgcctc gcacccacgg tttttccaac ccattagcgg
     2461 atcagaggtc tgtggcccgc gatagtcggg attgacctgc ggaatgacag cgtcggtatc
     2521 ctggagaaag ccctccagtt ctttggctaa tctgccgaca atctcgggat gttccgctgc
     2581 aacatcgtga gtttccccga tgtcctcacg aagattgtaa agttcaaaac gatctgtgcc
     2641 gtcaggattc ccggcgaaaa agcggatcaa cttccagtcg ccgtcacgca cataggctga
     2701 ggggagaaag cccggaattt gctgggcctg gttctctcca ccgtgcggaa agtggcaaaa
     2761 gacccgcgtg cggacagggc ctttgccaag aatggccggg acctgggaaa cgccatccac
     2821 tttgtgaccc ggcaaaggaa ggccacacat ctctagaatt gtgggaaaga agtccacact
     2881 ggaaaaaagt acgtccgtta ccgagtccgg ttgcgtgtgc cctggccaaa caatgatgag
     2941 cggtacccgc gtgccgcctt cgtagatgga ggccttaccg cttctcaacg gtgtgttgct
     3001 cgttgctgga atctccgcgt aaccaggcgg ttgcgtttcg ttctttgggg gataggcaaa
     3061 accaccattg tcagagaaga agatgataat ggttcgctcg gcaagaccgg ccttgtcgat
     3121 ggcctcgacg atgcgtccca ccgcgtcatc caggcttttc accatggccg cataaaccgg
     3181 gtttcgttgg ggcgagtcat ccttggcttt ggtcttaaaa tactcgacca actcgggttt
     3241 ggccgaccaa ggactgtgaa cggagaaaca ccagtagttc aggtagaaag gcttatcctt
     3301 gttttcctcg ataaatcgcg cagcctcttg agacatgcga tcctcaatgt gctctcccgg
     3361 ctggccttgg atggcagggt ccttgataaa cttccacggg gcgagatagc cacctccggg
     3421 acctggcgcc gccggggtat tagggaaatc gagcatgaag ccttgctcct ttggttcata
     3481 agggccggaa tgcccaagat gccactttcc aaaatgggcc gtcatgtatc catgttcacg
     3541 gaagacctcg gccaaggtga cgtactcggg tttaagccgg gttaggctat tggctgtacg
     3601 gatccggaca ttcggttgcg gtggaacaag gcctttttcc aggactgctt gtggaagatg
     3661 gcaagctggt gatgtgatac cgatccgcgc agggtagaga cccgtcaaga tgctggatcg
     3721 tgtgggagaa caaagcggat tagccgcgta ggcctgcgtg aagcgcagtc cgcgtctggc
     3781 gagacgatcg agattgggcg tctcatgata aacgctccca taaatcctca gatcgctcca
     3841 cccaagatcg tccgccaaga taaagaccac gttgggtggg ctggcaacgt ggtcttcgca
     3901 aactccccaa gctccctggc aaaccgacag gatggtggcg ataacggtcc aaagtagctt
     3961 ccatctcatg atgttcctcc ccaaaggtca tagcgtcatt ccaccaaaga atctcctggc
     4021 caataggata acacaaaggc gaagcgaaaa gccagccgag gagggattac cttttatggt
     4081 gtacagagtg ttgtagatcg gagacgtggc ccaaatatcg tgatcccgct agggtatcaa
     4141 atgccacaag gaggaatcgc cgcttttctc cgtgaatggc tgtcggtgcg actgtcgaag
     4201 tctttcttcg gttcttaact tgcctgcctg accgcgcgat cagttcgagg tgctgcccgg
     4261 tggcgggaag ttctcggcgg cagcgtcgat caccagcacc cagtcttgtc cttcaccctg
     4321 tgtcggcggc gtaaagtgca gctcatttcc cgaaaaatca ccctggatgg gtgtggcctg
     4381 accggtccgc gggtcaaacc accaggcccg ccgagctggg ccggccagag aatgtgtttt
     4441 caccgtaacc gtaccaccca tgggagaata gaccattgcg tatcgcccca attcgtcccg
     4501 cgtggcttgc aaatggtcgt ccccgtcgcc gagatcatct gtcagcagcg attggtcggg
     4561 aatccgcgtg aggaagggcc gtgactcgat taatcgtcgc aagtggacaa cctgaaaagc
     4621 gccgggggca tcaagcgact cgtaccaggg gatgtacgct ccatagcgat aggtttcacc
     4681 tttgcgaatc caaccgatga aactgcgatg gccatacgta aaaccgaagc caccagcgaa
     4741 aacgctccag taggcagcgc ggcggacatc ccactgacgg atgcgacgat cctgcggctc
     4801 ttgtcgatcg agcgacagtg aatcttcata agtgacctcg gcatccaggg tgggcttcgg
     4861 tggctggcgt tggtagtcgg cagtaacggt cttccagttt tggaaacgga atcgtgttgt
     4921 ggtttggatc atgttgaaat ccagccatgg ctcattgtgc agccattgtc ctggcgaacg
     4981 attacctccc ggcggatgaa aggtcatcaa ggccacgtcc cagttcgcat tgccgtcgaa
     5041 ttgatcgttt ccgataatac cgtcggcgat gccctcggcc acggcccgca ccagagccaa
     5101 gcgcgacggg ttatcgacat tacgtccttt ctcgtaggcg tcacctccca aaacccaaat
     5161 gatatgcggc tcgcgccgat aacgtttgcc gagaaagtag ccgtagcggt aagcgacctc
     5221 ggggttggtc tccatagggt gtcctggcgg gatggactga agccacatcg gcaggattgc
     5281 caatcgcaac ccgtgccgct tcgctgcctg aacgcaccaa tcgacatggt cccagtaatc
     5341 gtcgttttcg ccgggcttta tcaggggacg cgaccaatcg gtgtcaataa acggttcgaa
     5401 accgtaggca ttccggtagg agccgccaac aggctcacgg accaagacgg attggatgac
     5461 ggtaaagcct ttcttggcac gatttgcgaa gtacagttcc acagatggct ggttgtcctg
     5521 gccctcgtga acgctcttgc caaacatgtg ccagcacgtg tcgcctagcc agaaaaacgg
     5581 ttttcccgat accgtcacca gaaagcgacc gttgtcgctc accttaaggg gctccagttg
     5641 ctggaactct tcggcgaaga gtcgaaccga gccgcacggc caactgaata ggatggccaa
     5701 ggtcacaact accattagcc cagctccaca ttgctgccac gaaagcgcaa gagcgcattc
     5761 tttgccttgt ttgttcaaag ccataggtga tctccggttt gcgggtccaa ctaaacgcgt
     5821 ccggaagggc catttttaca agggatgccc acttgctccg gaaatacttg ctataagcgg
     5881 caccgaaccc tcggcaacgg cattgtagtg tgccgattct agttacccct cagggcaagg
     5941 tcaaccagcc attgattggt aaactgttt
//
LOCUS       DAOPTR010000078         5950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_10899_length_5950_cov_3.484818, whole genome shotgun sequence.
ACCESSION   DAOPTR010000078 DAOPTR010000000
VERSION     DAOPTR010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5950
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10899_length_5950_cov_3.484818"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2673
                     /locus_tag="PKI05_02570"
     CDS             <1..2673
                     /locus_tag="PKI05_02570"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOA51111.1"
                     /translation="AGSVLQFSGQGPLQVLGIRFERLGASPGNVVNVTGGEVLFAECR
                     FENGVYREGECWGAGLVLRGETHGIVRRCVFRRNQLGIVVTDQARPVLEENLCEGNTW
                     SGISYYGNASGKARKNTCRGNKLHGIYAGEQSQPVLEENLCEENGNCGIAYFGSASGK
                     AWKNTCRGNGLHGVYVSDQAQPELQENVCEQNGDSGIAYFRSASGKAWRNTCRGNKRY
                     GVYAGERAQPALGGNFCEGNAKCGIVYLDNASGKAWKNTCRGNKLHGVRVGDQAQPVL
                     EENLCEGNEECGMVYFDSAGGKARKNTCRANNVTGIWVDGQAQPELEENVCEGNGGCG
                     IVYIDSAGGKARKNTCRGNKLHGVEVSDQACPLLEENVCEKNQSSGIAYFGSASGKAW
                     KNTCRGNNGDGIYAGEQSQPLLEENLCERNAGCGIGYFESAAGKARKNRCRANKLDGV
                     KVRDQAQPQLKENLCEGNAKCGIAYVVSALGRAQKNTCCGNKLHGIAVSGQAQPELQE
                     NLCEGNGKCGIAYFESAGGKASKNTCRGNKLHGVYVTDQAHPVLQENVCERNGDCGIV
                     YLGSALGTAQRNICRGNGEDGIYAGEQSQPMLEENLCEGNGNCGIAYCDSASGKACGN
                     TCRGNISDGVKVDQRAQPVLEENLCEGNGCGIAYFGSAGGKAWKNICRGNKVHGFEVT
                     NEAQPVLEENLCEGNGECGIAYLNSASGKAWKNTCRGNENSGIWLADQAQPVLEENLC
                     EENDCGIAYLGSAWGRAWKNTCRGNKRDGVSVGGESLPVLEENLCEKNAFGIAYCGSA
                     AGRASKNTCRENKRHGVYVGGRAQPVLEENLCEGNGDCGIAYRGSAAGKAWKNICRGN
                     KNYDIYVGKSASPSLQENSLEKRSDKNTAKQESG"
     assembly_gap    2729..2828
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3563..4555
                     /locus_tag="PKI05_02575"
     CDS             3563..4555
                     /locus_tag="PKI05_02575"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOA51112.1"
                     /translation="MHHRIFPALKRTVSEIGIGTWQLGGTEWGDVPEDEALETLAAAA
                     DAGVTFIDTADIYGLGRSESLIGRFLKGRPDKDRFTVATKLGRHPEPGWPANFTREVV
                     RKHTEDSLRRLGVDVIDLTQTHSLPLEVMRDTGIFDFLRELRDEGKIRAFGASVESVE
                     EGLFCLGIEGMASLQIIFNIFRQKPIDALFDQAHQKGVALIIRLPLASGLLAGKFTRN
                     TTFPPNDHRAFNRDGQKFNVGETFAGLPFEKGLELVEQIRPWVPSGMTMAQMALRWCL
                     DFEAVTTVIPGAKRPDQARANAAASDLPTLPPILHNRLGRLYESQVAMHIRGKY"
     gene            4799..>5950
                     /locus_tag="PKI05_02580"
     CDS             4799..>5950
                     /locus_tag="PKI05_02580"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOA51113.1"
                     /translation="MKRCERMRPIILVIAICVVMSFCAGANLLAEEVPQATVRYNAAA
                     RLHNLQSWDLAATEWQGFIEGFPNDPRVGRAYHYLGVCLYNQQQYEKAAEAFRQSLTK
                     ELESDLRELSLLYRGIALFDAAQNGKAELYDAARSACQEYLKTYPRGKHAGEAAYYAA
                     ECLYFQDRKEQAAQAYRSFLQQFRDHPRTPEVLLALGVCEEELGNRQAALKDYQTFLQ
                     QYAQHPRATEVQYRLGEVLYALGNFAEAELAFRKAGADSRFADADLAVIRTGDTLVQL
                     KRFEEAAQQYAGVEKRFPQSSRLAQAILAAGRCYYLAGNYDSAIPLLVRASQSGEVQA
                     EAFHWLARCYLKKKQPEQVLATLREEIIGNAPAPWGAHLRLDLADALYEI"
BASE COUNT         1291 a         1352 c         1962 g         1245 t
ORIGIN      
        1 gccgggagcg tgctacagtt ttccggccaa gggccattgc aagtcctagg gatccgtttt
       61 gagcgtcttg gggcgtcgcc ggggaatgtt gtaaatgtga cggggggcga ggtcctgttc
      121 gcggagtgtc gctttgagaa cggggtttat cgggagggcg aatgttgggg agccggtctt
      181 gtgcttcggg gtgaaacaca tggcattgtg cgaagatgtg tctttcggcg gaatcagctt
      241 ggaatcgtcg taaccgacca agcgcggcca gtgttagagg aaaacctgtg tgaagggaac
      301 acttggtcag ggatttcgta ctatggcaat gcttcgggga aggcgcggaa gaacacctgc
      361 cgcgggaata agctgcatgg gatatacgct ggtgaacaat cgcagccggt gttagaggaa
      421 aacctgtgtg aggaaaacgg gaattgtgga attgcgtatt ttggtagcgc ttcgggtaag
      481 gcgtggaaga acacctgccg cgggaatggg ctgcacggtg tctatgtatc tgatcaagcg
      541 cagccggaat tgcaagaaaa cgtatgtgag caaaacggcg atagcggcat tgcgtacttc
      601 cgcagtgctt cgggtaaggc gtggaggaac acgtgccgcg gaaacaagcg gtatggggtg
      661 tatgcgggtg aacgggcgca gccagcatta gggggaaact tttgcgaggg aaacgcgaag
      721 tgtgggatcg tgtatcttga taacgcctcg gggaaggcat ggaagaacac ctgccgcggg
      781 aataagctgc acggtgtccg ggtgggtgac caggcacagc ccgtattgga ggaaaacctg
      841 tgtgagggga acgaggaatg cgggatggtg tattttgata gcgcgggggg gaaggcgcgg
      901 aagaacacct gccgcgcgaa taacgttacc ggtatttggg tagacggaca ggcgcagccc
      961 gagttggagg aaaacgtgtg tgaggggaac ggggggtgtg ggatcgtgta tattgatagc
     1021 gccgggggga aggcgcggaa gaacacctgc cgcgggaata agctgcacgg tgtcgaggtg
     1081 agtgaccaag catgtcccct gttggaagaa aacgtgtgcg aaaaaaacca gtcctccggg
     1141 attgcctatt ttggtagcgc ttcgggtaag gcatggaaga acacctgtcg cgggaataac
     1201 ggagatggga tatacgctgg tgagcaatcg cagcctttgt tagaggaaaa cctatgtgaa
     1261 agaaacgcgg gatgtgggat cggctatttt gaaagcgctg ccgggaaggc gcggaagaac
     1321 agatgccgtg caaataagct cgatggcgtg aaggtgcgtg atcaggcgca gccccaattg
     1381 aaggaaaatc tgtgtgaggg aaacgcgaaa tgcgggatcg cctatgttgt tagcgcgttg
     1441 gggagggctc agaagaacac ctgctgtggg aataagctgc atggtatcgc ggtgagtggc
     1501 caggcgcagc cggaattgca ggaaaatctg tgtgaaggaa atgggaaatg cgggatcgcc
     1561 tattttgaaa gcgctggcgg gaaggcgtcg aagaacacct gccgcgggaa taagctgcac
     1621 ggtgtctatg taactgatca agcgcacccg gtgttgcagg aaaacgtgtg tgagcgcaac
     1681 ggggattgcg ggattgtcta tttaggtagc gcgttgggga cggcccagag gaacatttgt
     1741 cgcgggaatg gcgaggatgg gatatacgct ggtgaacaat cgcagccgat gttagaggaa
     1801 aatctgtgtg agggaaacgg gaattgtgga attgcgtatt gtgatagcgc ttcagggaag
     1861 gcgtgcggga acacctgccg tggcaacatt tcggatgggg ttaaggtgga tcagcgcgca
     1921 cagcccgtgt tggaggaaaa tctgtgtgag ggcaatggct gcggtatcgc ctatttcggt
     1981 agcgctgggg ggaaggcgtg gaaaaacatc tgccgcggga ataaggtgca cggtttcgaa
     2041 gtgactaacg aggcacagcc ggtgttggag gaaaatctgt gtgagggaaa cggggagtgt
     2101 ggaatcgcgt atcttaatag cgcgtcgggg aaggcatgga agaacacctg tcgtgggaat
     2161 gagaacagcg gtatctggtt ggctgaccag gcgcagccgg tgttggagga gaacctgtgt
     2221 gaagagaatg actgtgggat cgcctatttg ggcagcgctt ggggcagggc atggaagaat
     2281 acgtgtcgcg ggaataagcg tgacggagtt tcggtgggtg gggagtcgct gcccgtgtta
     2341 gaagaaaacc tgtgtgagaa aaatgccttt gggatcgcct attgtggtag tgccgcgggg
     2401 agggcatcca agaatacctg ccgcgaaaac aagcggcacg gagtgtacgt gggtggaagg
     2461 gcacagcccg tgttagaaga aaacttgtgc gagggaaacg gtgattgcgg catcgcgtac
     2521 cgtggtagcg ccgccgggaa ggcgtggaag aacatctgcc gcggtaacaa gaactacgac
     2581 atctacgttg gaaagagtgc gtcgccatcg ttgcaggaaa atagcttgga gaaacgaagt
     2641 gacaaaaaca cggcgaaaca agagagtggc taaatagtgt tctgccggaa gcgtagtcaa
     2701 tcgtcggggc aaccccctgt ggttgcccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnat agtgttctgc cggaagcgta gtcaatcgtc ggggcaaccc cctgtggttg
     2881 ccccatttgt ccgcagcgcc ggtggtttgc gtttggccgg gcccggattg tttggatttc
     2941 acacgcaccc gggcgttatt aacgctaatc gcattttggg gcaggcacgg gggcctgccc
     3001 ctacggttgt tggttttcta ttcgcgaagc cgtaggggca accccctgtg gttgccctcg
     3061 tcgtccgcat ggctggtggt ttgcgtttgg ccaggtgccc aggttgtttg gatttcgtac
     3121 tgacccgcgg ggtatttgtg ctgcggggca ggcacagggg cctgccccta cggttctgat
     3181 gggcccctat tcgccatggt caacgggtat atcgaaccgg acggcgacga ccgcgtccct
     3241 cccacgggcg cgcagaaaaa cgcggtggac gcgacggagc gagtccctcc caaaacggcg
     3301 gtggtggagg gccacgcttc gtcttgggca gcttgaccat ttccctgtac gtgtaccttg
     3361 tacgtgcttg cgttgtggca tcctctcggg caaactgaga tttacgcaag atgacggtac
     3421 ttggcatcga tcagtggcga agaaagtgag ccccgtcccg tgtcaactgt aaggagttct
     3481 ctgccctgga gtgtagtatt tccgcatcaa aggtgtccgc atcgcaaacg aaaaccttga
     3541 tcgtttccca aaaggaggtg acatgcacca ccgcatattt ccagccctta agcgaacggt
     3601 ttctgagatc ggaattggca catggcaact cggtggcacc gagtggggtg atgttcccga
     3661 ggatgaggcc ctggagacgc tggccgcggc agccgacgcc ggtgtgacgt ttatcgacac
     3721 ggccgacatt tacggtcttg ggcggagcga atctctcatt ggtcgttttc tgaaaggccg
     3781 accggacaag gaccggttca ctgtggcgac gaaactcggt cgtcaccccg aaccgggctg
     3841 gcccgctaac tttacccgcg aggttgtccg aaagcacacg gaggactctt tgcgacgcct
     3901 gggggtcgat gtgatcgact tgacgcaaac ccacagtttg cccttggaag ttatgcggga
     3961 tacggggatt ttcgatttct tgcgcgaatt gcgggacgaa ggcaaaatcc gcgcctttgg
     4021 agcgagcgtg gaaagtgtcg aagaaggcct gttctgtctt ggcatcgaag gaatggcttc
     4081 acttcagatc attttcaaca tctttcgtca gaaaccgatc gacgcccttt tcgatcaggc
     4141 acatcaaaag ggggttgctc tgattattcg gctgccttta gccagtggtt tgttggcggg
     4201 gaagttcaca cgaaacacca cgtttccgcc caacgatcat cgtgcgttca acagggatgg
     4261 tcagaagttc aatgtgggag agacatttgc ggggttgccc tttgaaaaag ggctggagtt
     4321 ggtggagcag attcggccat gggtcccttc cgggatgacg atggcccaaa tggccctccg
     4381 ctggtgtttg gatttcgagg ccgtgacgac cgtcatcccc ggggcaaagc gacccgacca
     4441 agcccgcgcc aacgccgctg ccagtgattt gcccacgctc ccgccaatct tgcacaaccg
     4501 cctgggacga ttgtacgaaa gccaggtggc tatgcatatc cgcggcaaat attgaaaagg
     4561 ggtttgcttt gccgacgggg gttgtggcgg gcaccgccgg ttcccctcca ccggcgaaaa
     4621 agcactttcc gagggacgtt tggacgcgat atgatgacga aaggcgtttt cgtcatcccg
     4681 tttggtgttg cgctgtcggg aattcatctt tctgcccacc ggcgccgacg cgaattgtaa
     4741 gtcgcggccg gaatggatta ttgccctggc gacgtgatgc aagaggagtg aggagggcat
     4801 gaaacgttgc gaacgtatga gaccaatcat actcgtcatc gccatttgtg tggtaatgag
     4861 tttttgcgcc ggcgcgaatc tcctggccga ggaagtacct caggcgaccg tccggtacaa
     4921 cgccgcagcg cgactacata atctccaatc ctgggatctg gcagcaacgg agtggcaagg
     4981 tttcattgag gggtttccca acgatcctcg ggtggggcgg gcatatcact atttgggggt
     5041 ctgtttgtac aatcagcagc agtacgaaaa ggcggccgag gcctttcgac agtcgctgac
     5101 caaggaactt gagagcgatc tgcgcgaact ttctctgtta taccgaggga ttgccctatt
     5161 cgatgcggct caaaacggca aggcggaatt gtatgacgcg gcccgttctg cctgccaaga
     5221 gtatctgaaa acctatccca ggggaaagca tgcgggtgaa gcggcgtact atgcggccga
     5281 atgcctctac tttcaggacc gcaaagagca ggccgcgcag gcttaccgga gttttcttca
     5341 acagttccgt gatcatccgc ggacccccga ggtcttgtta gccctgggag tgtgtgagga
     5401 agaattgggg aatcgtcagg cggcgctgaa ggactatcag acttttttgc agcaatacgc
     5461 gcagcatccg cgggctacgg aggttcagta tcgtttggga gaggtcttgt acgccttggg
     5521 caatttcgct gaggcggaac ttgcctttcg caaggccggg gctgattcgc gtttcgccga
     5581 tgccgacttg gccgtgatcc gtactggcga taccctcgtc caattgaaga ggtttgagga
     5641 ggcggctcag cagtacgccg gcgtggaaaa gcggttcccg cagtcctcac gactggctca
     5701 agcgattctg gcggcgggcc gatgttatta ccttgccggt aattatgatt cggcaattcc
     5761 tctcttggtg cgcgcgtcgc aaagcggtga ggtgcaggcc gaggccttcc attggttggc
     5821 ccgctgttac ctcaagaaga aacagcctga acaagtgctc gccacattgc gggaagagat
     5881 tatcggtaac gcgcccgcac cttggggagc ccatctgaga ttggatctgg ccgatgcctt
     5941 atacgagatt
//
LOCUS       DAOPTR010000079         5897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_11034_length_5897_cov_3.337042, whole genome shotgun sequence.
ACCESSION   DAOPTR010000079 DAOPTR010000000
VERSION     DAOPTR010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11034_length_5897_cov_3.337042"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="PKI05_02585"
     CDS             complement(<1..1078)
                     /locus_tag="PKI05_02585"
                     /inference="COORDINATES: protein motif:HMM:NF027900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HOA51114.1"
                     /translation="MRNQFPTASRAKHWASVTSLVWNLVGVGCLLMLLADRGQVPKAV
                     GEEHQQSPRPGLQVMVEPNTERLTAYQIFELTFRHSGHYQNPTWDVSIEVILRSPTGK
                     QYRVGGFYYGPAEEETADRTRDGGKSLEANLPNESRGPLQVWKARYAPSEFGQWSYEY
                     RFRNPFGEEAVGTGTFNVVRTRASFPGWLRISKDNPYRFVFEDGSPFFPVGFQDCEGD
                     HNKNGTTLDSKSMEGPFRLDKATERPQPPPGSLFARGPSMNPVNGDVYFGKHARAGFN
                     IFRFSPHNCSISVFDLDRVDYREAKMVDQLLEMVRKYRIRIYYGIFGFAKVFNDQPDN
                     AEGMRKVRRLIKYSVDRWGAYVDIW"
     gene            1691..3724
                     /locus_tag="PKI05_02590"
     CDS             1691..3724
                     /locus_tag="PKI05_02590"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HOA51115.1"
                     /translation="MHRKPATIFWALTVGTMLWLMQTLPVPSEERPPWLGNRAGISDE
                     LLAPWTPVEVKDTTISVWGRSYSFGPLPLPAEVTTAGAEILAGPVTLHCTADGQPITW
                     SAGGRSIVEHFPTRAILRFSAQGGPLRCEGEVTTEFDGMIRCGLRLVPVADKITIENL
                     ILEIPFKSKHAVFMHSWPGRWGSAANSHAVPPVEWQSAFKPFVWIGDYERGLAWFCES
                     DENFFPASLNRVIRVRRDGEITHQRVELIGKAQTVSQPLRYVFGFQATPVKPLQPDAW
                     DYRIVHVGRYGLEKQMFSPPGQAPKLLLDYLAEAGVKTICFHEHWSNIQNYPQAARPE
                     ELHSLVEACHKRNIQLLLYFGYEMSNIAPEWEKYAEECLVAPRAGGYKRQPEQTAYIV
                     CYRSHWQDFLAAGIERIIREFDIDGVYLDGTSEPWGCTNTQHGCGYTRPDGSIGPTYP
                     FYDTRQMMKRIYTIVKHHNPAGQVNVHQSTCMVIPTLAFATSYWDGEQLQSVARPDSP
                     TKLLPLEAFCAEFMGRNWGVPGELLWYANGPFKWEEAVSMALLHDIPVRPNVDRLELF
                     RRLWRLFDEFDRHASQWIPYWQSSGVVACSHPEIKVSLYNRPGNGCILVAVNVGTEEI
                     QADLTLGLSALGQSDLTIWDVLTDKEIGPLGEKMPLKLGPWQHAIFWLKPRRS"
     gene            complement(4129..4593)
                     /locus_tag="PKI05_02595"
     CDS             complement(4129..4593)
                     /locus_tag="PKI05_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOA51116.1"
                     /translation="MSWRLGFAGLLILLLAIAGCGSKGPKLARVRGKVTLDGQALPNA
                     LVTFMPEGGGVASSGTTNANGEYELVCPQGRGAVVGKHKVSVRTLPPQQSQAQEVSSD
                     DPSYLAQYSGQSSATAQPFVEKIPPRYNTQTELVKEVKPGSNEINLDLTTSP"
     gene            complement(4736..>5897)
                     /locus_tag="PKI05_02600"
     CDS             complement(4736..>5897)
                     /locus_tag="PKI05_02600"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOA51117.1"
                     /translation="RNCIGYWRFLTPGVMFVGFCNGLEGYEMRNKVRFRVGFTLVELL
                     VVIAIIGILIALLLPAVQAAREAARRSQCTNNLKQIALAVHNYIDVYKVFPALGVRTC
                     RGRYYAWSMMILPYLEQKPLYDAIMAQAQSPDMLPEPWHSTQNVPSSPASWAPFVRQW
                     WQIDIRTYVCPSDQPIPNRQESPARLNYKGCVGDDYHQNHFRPDQNNRQNRGIFQCER
                     YVGLEMVTDGTSNTIMLGEMVAGGYPDDIKGGVALNMTAWNPAACLARVDPATKKLIP
                     PVRADFRPVGGRAWDGRPYFTGFATLVAPNGPSCHWGGVDGNEHMGTASSFHPGGVNV
                     AMADGAVKFISETIDVGNQAVDDVDTPGNRRSPWGVWGALGSAYGGESVTAP"
BASE COUNT         1319 a         1705 c         1571 g         1302 t
ORIGIN      
        1 cccaaatatc aacgtatgcc ccccaacggt ccaccgagta cttaatcaac cggcgaactt
       61 ttctcatccc ctcggcattg tcgggttgat cgttgaagac ctttgcaaaa ccgaagatgc
      121 cgtagtagat ccggatacga tacttgcgga ccatctccaa aagctggtcc accattttgg
      181 cttcacgata atcaacacgg tccaaatcaa agacgctgat ggaacaattg tggggcgaaa
      241 accggaagat attgaatcca gcccgagcgt gcttgccaaa gtacacatca ccgttgacag
      301 gattcatcga ggggcccctt gcgaacagtg accccggcgg aggctgaggg cgttccgtgg
      361 ccttatccaa gcggaaaggc ccttccatgc ttttgctatc aagagtggta ccgtttttgt
      421 tgtggtctcc ttcgcagtcc tggaaaccca caggaaagaa cggcgacccg tcctcgaaca
      481 cgaaccggta agggttgtcc ttactgattc gcaaccagcc ggggaaagac gcccgtgtcc
      541 gaaccacgtt gaaagttccc gtccccacgg cctcctcccc gaaaggattg cgaaaccgat
      601 actcgtagga ccactggccg aattcgctcg gtgcgtagcg agccttccaa acctggagtg
      661 ggccacgcga ctcgttgggc aagttcgctt ccaaagattt gcccccgtct cgggtgcggt
      721 cagcggtctc ttcttctgcc gggccataat agaaaccgcc cacccgatat tgtttgcccg
      781 ttggtgaccg gagaataacc tcgatagaaa cgtcccaggt ggggttttgg taatgacctg
      841 aatgtcggaa cgttagctcg aatatctggt aggcggtaag cctttccgtg tttggttcga
      901 ccatcacctg caagccaggg cgcggcgatt gttggtgctc ctcaccgacg gcctttggca
      961 cctgcccccg gtctgctaac agcattagga ggcatccgac accgacaaga ttccaaacta
     1021 gtgaggtgac gctagcccaa tgcttggcac gactcgcagt gggaaattga tttctcatgg
     1081 attgtcctcc atattgaacc gactccgaca aaactcgttt tctctccgcc ggcgccgaca
     1141 tactaccatg gtagtactct ccctttcaag ccgtgcctgg ccggaagtct ttcgcgcaat
     1201 gcggtgcgct aaaagagcct atcccatgat ctcccctgaa gccttcggcg ggaaaggggt
     1261 cgggggtgag ggcggcccga atcgatgccg tttttctgaa ttgattccga cgttcgcttg
     1321 ccctcaccct aaccctctcc cggagggagg gggggctttt tgggataggt tctaaaagta
     1381 gggtgacaac ctcgggatgc ggtatcggcc gtaccggcac tggaacaagc gtgttcgtcc
     1441 aaaccttcat agctcagcga agaggttctg tctcattggt tctgccgtcg ctttcacgtc
     1501 cttcttttgg cgtgggcaat tggcccttac actagtgtgg ggaaactgct gtgggaagac
     1561 tgggagaaag acttatcggc gatccggagg agaaccgctc gcccacaatg gtcaattctt
     1621 actcgcagtg gaatacggca cgtgtgggat aaaagtgaag cgacgagtcc gatcctgagg
     1681 agagaaaacc atgcatcgaa aaccggccac catcttttgg gcattaaccg tggggactat
     1741 gctgtggttg atgcagactt tgcctgttcc atccgaggag cgaccaccct ggctaggaaa
     1801 tagggctggg atcagcgacg aactgctcgc cccatggact cccgttgaag ttaaggacac
     1861 gaccatctcg gtatggggac gtagttatag cttcggacca ttaccgctac cggcggaggt
     1921 gaccacagcc ggggccgaga tcctggcggg accggtgacc ctgcattgca ccgctgacgg
     1981 tcaaccgatc acttggtcgg ctggtgggcg gtcgatagtg gaacatttcc ccacacgtgc
     2041 catattgcgg ttctctgctc aagggggtcc ccttcgctgc gagggggagg tcaccacgga
     2101 atttgacggc atgatccgct gtggtctgcg tctcgttccg gttgccgaca agatcaccat
     2161 tgaaaatctg atccttgaaa ttccctttaa gagcaagcat gcggttttca tgcacagctg
     2221 gccgggacgg tggggaagtg cggccaactc acacgccgtc ccgcctgtcg agtggcagag
     2281 cgcttttaaa ccctttgtgt ggatcggtga ctacgaacgg ggattggcgt ggttctgcga
     2341 atccgacgaa aacttctttc ccgctagcct gaaccgcgtg attcgagtcc gtcgcgacgg
     2401 agaaattacc caccagcgag tggaactcat cggtaaagca caaaccgtca gccaacccct
     2461 gcgatatgtc ttcggcttcc aggctacgcc cgttaagccg ctacaacccg acgcttggga
     2521 ctaccgtatc gtccatgttg gccgatatgg tctggaaaaa cagatgtttt ctcccccagg
     2581 acaagccccg aagctgctct tggattacct ggccgaggcg ggcgtcaaga cgatctgttt
     2641 tcatgaacac tggagcaaca tccagaatta tccccaggcg gcacgtcccg aagagttgca
     2701 tagccttgtg gaggcctgcc acaagcgcaa tattcagttg cttttgtact tcgggtatga
     2761 aatgagtaac attgccccag aatgggaaaa gtacgccgaa gagtgcctgg ttgctccacg
     2821 ggcaggcggt tacaagcgac aacccgaaca aaccgcgtac attgtttgtt accgcagtca
     2881 ctggcaggac tttctcgctg ccggtatcga gcggatcatc cgggaattcg acatcgatgg
     2941 tgtgtatctc gatgggacat ctgaaccatg ggggtgcacc aatacccaac acgggtgcgg
     3001 ttacactcgc ccggacggct caattgggcc aacttatccg ttttatgaca cacggcaaat
     3061 gatgaagcgg atctacacca ttgtcaaaca tcacaatccc gccgggcaag taaacgtgca
     3121 ccaatccacc tgcatggtta ttcctacctt ggcctttgct acaagctatt gggacggtga
     3181 acaattgcaa agcgtggccc ggcccgattc gcctaccaag ctcctcccgt tagaagcatt
     3241 ctgcgcggag ttcatgggga gaaactgggg cgttccgggc gaactcttgt ggtacgccaa
     3301 cgggccgttc aagtgggaag aagcggtgtc tatggccctt ttgcacgata tccctgtgcg
     3361 accgaatgtc gaccgccttg agcttttccg acgcttgtgg aggctctttg acgagttcga
     3421 tcggcacgca agtcaatgga ttccttactg gcagagctct ggcgttgtcg cctgttctca
     3481 tcctgaaatt aaggttagcc tctacaaccg gcccgggaac ggttgcatac ttgtggccgt
     3541 caacgtcggc acagaagaga ttcaagccga cctaacgttg ggattgtcgg cgcttggcca
     3601 aagcgacttg accatctggg atgtgctaac ggacaaggaa attgggccgc ttggagagaa
     3661 aatgcccctc aaactgggac cgtggcagca cgccattttc tggttgaagc cacgccgctc
     3721 ctaaaggcca catccctccg cggatcaagg ccatctgact ttggttatgg tcctggccgg
     3781 aataggaaat gtctcgcggt cctcgctctt ttcgtatgcg tatttccgcc caggaaggct
     3841 cgcgctggct aggccaacca cgccacttgc ctgacctgcc atcgttaaga tggccgattt
     3901 gtgaatagcg cagtccggca gctccacccc caccttggca ccccggacgt acggccagct
     3961 tctcgaaatc tcatctcacc tctttcggcg accaccgggt gggaaaagag ctaccccctc
     4021 gtctgaaggc acgtcttaac cgctcctgac ctgatcaggc cgaggacgtt ctcagtggcg
     4081 cgcggccttc ttgcgcacac tattgcgcat cctgaaaata acagccactc agggcgaagt
     4141 ggtcagatcg aggttgatct cattgcttcc gggtttgacc tccttcacca gttccgtctg
     4201 cgtgttgtac cgtggtggga ttttctccac gaacggttgg gccgtcgcgc tgctctggcc
     4261 actgtactgt gccaaatagg atggatcgtc cgacgacacc tcctgagctt ggctctgttg
     4321 cgggggaagt gtccggacgg atactttatg tttgcccaca acagccccac gtccctgagg
     4381 gcagacaagt tcatactcgc cattggcatt ggtcgtaccg gaggacgcca ccccgccccc
     4441 ttccggcatg aacgtcacca aagcgttggg cagcgcttga ccgtctagcg tcaccttgcc
     4501 gcggactcgt gccagtttcg gtcccttact gccacaacct gctatcgcaa gtaaaagaat
     4561 caataacccc gcaaatccca aacgccagct catatcagct gctcctggaa tgtgtaacgg
     4621 ttcaatatca actggaatcg tgtgacggtt aaacatcaaa acgcaacggt ggagcgaaat
     4681 gctttgcctc cagctcgaac agttgttggt aaaacgctcg ccctaaccac ccctatcacg
     4741 gtgcggtcac cgattcgcca ccgtaggcac tccccagggc tccccacaca ccccaaggag
     4801 atcggcgatt accgggcgtg tcaacatcgt cgaccgcctg gttacccacg tcaatcgttt
     4861 cgctaatgaa tttgacagca ccatcggcca tggcaacatt gacaccaccg ggatggaaac
     4921 ttgaggctgt acccatgtgc tcgttcccat cgacgccacc ccaatggcaa ctaggtccgt
     4981 tcggcgctac aagggttgca aaaccggtaa agtatggccg accatcccat gctcgtccac
     5041 caacgggccg gaagtctgca cgaaccggag gaattagttt tttggttgcc ggatccacac
     5101 gggcaagaca ggccgctggg ttccaggcag tcatattcaa ggccacacca cctttgatgt
     5161 catcgggata tcctcccgcc accatttccc caagcatgat ggtgttggag gtaccgtcgg
     5221 tgaccatttc caaccctaca taacgctcgc actgaaaaat accacgattt tggcggttgt
     5281 tctggtcagg ccgaaagtgg ttttggtggt agtcatcccc cacacacccc ttgtagttca
     5341 ggcgagccgg gctctcctgt cggttcggta tcggttgatc ggacggacac acataagtcc
     5401 ggatgtctat ttgccaccac tgcctcacaa aaggcgccca ggaggcgggg ctggaaggca
     5461 cgttctgcgt ggaatgccac ggttccggca acatgtccgg cgactgagct tgcgccatga
     5521 tagcatcgta aagcggtttt tgctcgagat aaggaagaat catcatcgac cacgcatagt
     5581 aacggccacg gcatgtccgc acacccagcg ccggaaagac tttgtaaacg tcaatgtaat
     5641 tgtgaactgc cagcgcaatc tgctttaggt tgttggtaca ctggctccgt cgggcagcct
     5701 cacgggcagc ttgaacagct ggaagcaaaa gcgcgatcaa aataccaatg atagcgatca
     5761 ccactagcaa ctcgaccaag gtgaagccca cacgaaacct tactttgttc ctcatctcat
     5821 aaccctccaa cccattacag aacccaacaa acatcactcc tggcgtcaga aaacgccaat
     5881 aaccgataca attacgg
//
LOCUS       DAOPTR010000080         5816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_11247_length_5816_cov_3.541399, whole genome shotgun sequence.
ACCESSION   DAOPTR010000080 DAOPTR010000000
VERSION     DAOPTR010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5816
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11247_length_5816_cov_3.541399"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PKI05_02605"
     CDS             <1..486
                     /locus_tag="PKI05_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOA51118.1"
                     /translation="VIQSRADGLGNPVTVASPWQVVLAIGMCALAGLIYGWLSVVIRR
                     VTTTGTSGWFVLVLVTGVGVVTMGPMTLWNHGFQIVRQTAGEHWLWMIVSGLLNLIGF
                     WALTKGLQMTPVARANLLTSSQAAIAAVAGWLIFGEVLNLTVGLGLALTLCAIVLSSL
                     K"
     gene            complement(510..1340)
                     /locus_tag="PKI05_02610"
     CDS             complement(510..1340)
                     /locus_tag="PKI05_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOA51119.1"
                     /translation="MPIQITSLRNPRVKEAAALREARERRQRQEMIIDGVREIRRACE
                     AGIKLKEVFFCASLLTSEGKQLLSQVSESETELFEVTSRVFEKLNFGDRQDGIVAVAA
                     TPQRSLAEIEDQTGTALVAILEAIEKPGNLGAIIRTADGAGLSGVIAADLRTDLYNPN
                     TIRASLGTIFSMPVVAMDSRSAREWALARGLQLVAARVEGTIRYDQVDFTRPTGIVLG
                     NEATGLTPIWKRADVLGVRIPMLGQADSLNVSVAAAILFYEAQRQRGFPIPARRKRER
                     "
     gene            complement(1407..5411)
                     /locus_tag="PKI05_02615"
     CDS             complement(1407..5411)
                     /locus_tag="PKI05_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51120.1"
                     /translation="MKPEKSASPVEVILGYLNFSQGVRDPRFFAAWNRLWTLREIAFL
                     SQPSEAQTQERPELLHRYLDNELSRLRRENPAFSDTAQAEYVLEFLFERLIHGYRQFH
                     RDILAHLTLDDYCQPFLLARFCEAILAELHEAELDGKADDNEMLEATIRRLNDYLGYR
                     PVPTLEGKKRHKPYEHEFVCPIPLWIRGAGAAVGPYQRLIETALGILRETSAELCRAS
                     YFDFEQLEEIVLDPRGYDMEHPVHQRPNYLYGLWDPRKLNDRGYYCRFVLHDVHLNSI
                     LARVLEGPADDYEERLFEGAAVLAGTILMASGISGDRPGAILSSVQIDKWLQGIALVR
                     DQFYIELLGRCTGERRQRLESEMQRLRQPFGATRQALNQKLAARRSQELYVVSLATCF
                     AKMGAVEEARYWMQQIHSGAARIRCEIRCQLGECEWLIRNNRRTEALKTLSGVIELIH
                     RGIECGALIDPWCILGFGCLFPIFEHLREGVFDYRVEQLVGLVRLVFQRLAALRRAAA
                     ASGDEAVDEAAENLAAELADWWDQFATTAVADANSFLGRDECTAATVVSRILKRWREE
                     PEAAQSLAFWKEHAGSLQSAASYVLLVETLLQHRDTTAAMALLVDWASRADEVGLGVG
                     EQSFQVMALRWFQTIWTEQKDIKDRWKWTQKFIDYLEANCESLFRVPNLSEFFKRAGS
                     PVIRSEDEENGDDDVLSAAYEGVIFRDSAADGHEGDTLDDEISDSELAPLLGEISDRC
                     NFLSATTLIRYDALLASFRAISEVPDSATILMTWYRDAQEAEERLLQLAEEIWRYTLV
                     PPSPSPEGMMEFERQRQIRDALVDRVIQTAINVLSLRYLLRAIINVEATEDESQQPQW
                     DTYYPRLLRAVLYGDEAAFHQLMESFAKVIVTRPILYFSLVRGGNPRTYFLARGTHRA
                     LGLLAYLIARAGWVNAAMGLLSLVKSAERHQSEGKGKVSEIEHLFEAVAEGIFLALVF
                     AKRNGAKRKEGRLSRQETEDLVADLHAVCNGLLEIWAVIIHEIYFSTVETAPFRRSWT
                     QIRQFIRAFGQEIFTPMFMAHPNLRAIMATGTHHWLKMVKESGDAQWPRLVEALRSGE
                     LKLKKAAEYLRLIIDVILENYEDYLDYNSMTTQSDYGQNLYILLDFLRLKCEHQRRAW
                     MLKPFLLAHRVLVRLELWEAAEIFEEHVQSQYQGWSKLDLKRYEEMRQSYGIHLPGLY
                     EILSNGFGFSFAMQHVLGLIRPAMQEIRTGQTAGRLAMLVERIRSFGPFHVRYGFHRP
                     EWIDELENTIDRAELNLEVLDQPWEWLRTAWGQVGISSRESAEILHEVSQWGHLVRNS
                     SMISFTLPEDDRSK"
BASE COUNT         1328 a         1730 c         1486 g         1272 t
ORIGIN      
        1 gtgattcaat ccagagccga tggactggga aaccctgtga cggtcgcttc gccctggcag
       61 gtcgtgttgg ccatcgggat gtgcgcccta gctgggctta tctatggctg gttgagcgtc
      121 gtaattcgcc gcgtgaccac caccggtacc tccggctggt ttgtcctagt tctggtaacc
      181 ggcgtgggtg ttgtgacgat gggccccatg accctctgga atcacggctt tcagatcgtg
      241 cgtcagacgg ccggtgaaca ttggctgtgg atgatcgtct cgggacttct caacttgatc
      301 gggttctggg cattgaccaa aggtttacag atgacgcccg ttgcccgggc caatctcctc
      361 acatcctctc aagccgctat cgccgccgtg gcaggttggt tgattttcgg tgaagtcctc
      421 aatctcactg tcggcttggg cctcgccctg acgctctgcg ccatcgtcct gagtagtctc
      481 aagtgaacgt cccacctcca gtggtcagtt caacgttcgc gcttccgccg tgccgggatg
      541 ggaaaaccgc gctgtcgttg agcctcgtaa aagagaatcg ctgcggccac cgagacgttc
      601 aaactatcgg cctgtcctaa catgggaatc cgtacgccaa ggacatctgc tctcttccag
      661 atcggtgtca gcccggtggc ctcattccct agcacaatcc ctgttggacg tgtgaaatcg
      721 acctggtcgt accgaatggt accctcaaca cgtgccgcca ctaactgaag gccccgcgcc
      781 aatgcccatt cccgtgcact gcggctgtcc atggcaacga ccggcatgga gaagattgtc
      841 cccaggctgg cgcgaatggt gttgggattg tacaagtcgg ttcgcagatc ggccgcaatc
      901 actccgctaa ggcccgcacc atccgccgta cgaatgatgg cccccaggtt gcccggcttt
      961 tcgatggctt ccaaaatcgc gactagggct gtaccagttt ggtcctcaat ttcggccagg
     1021 ctccgttgcg gggtggcagc cacggcaaca ataccgtctt ggcgatcccc aaaattgagt
     1081 ttttcaaata ctcgggaggt aacctcgaac aattctgtct ccgattcgct aacctgagac
     1141 aagagttgct tcccttccga ggttaacaac gaggcgcaga aaaagacctc tttcaattta
     1201 atacctgcct cacaggcccg acgaatctcc cggacgccgt caataatcat ctcctggcgc
     1261 tggcgtcttt cgcgggcctc tcgcagagcc gctgcctcct tgacacgggg atttcgaaga
     1321 ctagtgatct ggatcggcat aggttcccga tatctcggtg aatcgatggt acaccttggt
     1381 tttcacggca accttatgac tgatgcttac ttcgagcgat catcttcggg gagagtgaat
     1441 gaaatcatag aactattccg cacgagatgg ccccattgcg agacctcgtg gaggatttcg
     1501 gcactttctc gagaggaaat ccccacctgc ccccaagctg tccgtaacca ttcccatggc
     1561 tgatcgagaa cctccaaatt caactcagcc cggtcgatgg tattttctag ctcgtcgatc
     1621 cattcggggc gatggaagcc atatcggaca tggaagggcc cgaaggagcg aatcctttcc
     1681 acaagcatgg cgagccgccc cgcagtttgg cccgttctga tctcttgcat ggctggccga
     1741 atcaggccca acacatgttg catcgcaaag gaaaaaccga aaccgttgct taagatttcg
     1801 tacaggcctg ggaggtggat accgtacgac tgtcgcattt cctcatatct ttttaggtcg
     1861 agcttcgacc aaccttggta ctgtgattgc acgtgttcct caaaaatctc ggctgcctcc
     1921 cacagttcca gccttaccag gacgcgatgg gccagaagaa acggcttgag catccacgct
     1981 cgtctttgat gttcgcactt cagccggagg aaatcgagca ggatatagag attctgcccg
     2041 taatcggact gagtcgtcat gctgttgtaa tctaaatagt cctcgtagtt ctcgagaatg
     2101 acatcgatga tcaaacgtag gtactcggcg gcctttttga gcttcaattc cccggatcgc
     2161 aaggcctcca ccaagcgtgg ccattgggcg tcacccgact ctttgaccat cttcaaccag
     2221 tgatgcgttc ccgtcgccat aatagcgcgg aggttaggat gggccataaa catcggggtg
     2281 aagatttcct gcccaaaggc gcgaataaat tggcggattt gcgtccaaga ccgccgaaaa
     2341 ggagccgtct ccaccgtgga gaagtaaatc tcgtgaataa tgacggccca aatctcgagc
     2401 agcccgttgc aaactgcatg caagtcagcc accaggtcct cggtttcctg tcgcgacagg
     2461 cggccctctt ttcttttggc tccatttcgc ttagcaaaaa ccaaggccaa gaaaatccct
     2521 tcggcgacgg cctcaaataa atgctcaatc tcagaaactt tgcccttgcc ttcggactgg
     2581 tgacgctcgg cacttttcac cagactcaaa agccccatgg ccgcattgac ccatcccgca
     2641 cgggcgataa gataggccag caaacccaag gcccggtgcg tgccacgcgc caaaaagtat
     2701 gtccgcggat ttccaccacg gacaagcgag aaataaagaa taggacgggt aacgatcacc
     2761 ttggcaaaag attccatcaa ttgatgaaag gcggcctcgt ctccgtacaa aacagcccgc
     2821 aaaagccgcg gataataggt atcccactgt ggctgttggg actcatcctc tgttgcctca
     2881 acgttgataa tggcccgtaa caaatatcgc aagctaagga cgtttatcgc tgtttgaatg
     2941 acgcgatcca ccagcgcatc acgaatttgg cgctgccgct caaactccat catgccttcc
     3001 ggactcggag acggcggcac gagggtatat cgccaaatct cttctgccag ttgcaacaac
     3061 cgttcctcag cttcctgggc gtccctgtac cacgtcatca ggattgtcgc tgagtcaggc
     3121 acttccgaaa tggcacggaa cgaagccaat agggcatcgt atcgaatcaa ggtcgtggca
     3181 cttaagaaat tgcagcgatc agaaatttca cccaatagcg gcgccagttc ggaatcagaa
     3241 atctcgtcat ccagtgtgtc gccctcgtgc ccatcggcag cactgtcgcg aaagatcact
     3301 ccttcataag ccgcactcaa cacatcgtca tcaccgtttt cctcgtcctc ggaacgaatg
     3361 acagggcttc cggcccgctt gaaaaactct gagagatttg gcacacgaaa cagggactcg
     3421 caattggcct ccaagtagtc gataaacttt tgggtccact tccagcgatc tttaatgtcc
     3481 ttttgttctg tccaaatcgt ctgaaaccac cgcagggcca tgacctgaaa cgattgttcg
     3541 ccgactccca atcccacttc gtccgccctg cttgcccaat ctaccaagag cgccatcgct
     3601 gccgtcgtat cccgatgctg caggagcgtc tcgacaagca agacataact ggcggctgac
     3661 tgcaacgagc cggcgtgctc tttccaaaac gctaggcttt gggcggcctc cggctcttct
     3721 cgccatcgct tcaaaatacg actgaccact gtagcggcag tgcattcatc gcgtcccaga
     3781 aagctattgg cgtcggccac cgccgtagtg gcaaactggt cccaccaatc ggcaagctca
     3841 gcagcaaggt tttcggcggc ctcatcaacg gcttcgtcgc ctgaagcagc cgcagcccgg
     3901 cgcaaggccg ccaatcgctg gaaaacaaga cgaaccagac cgacgagttg ctcgacccgg
     3961 tagtcaaaga cgccctcccg caaatgctcg aaaatgggaa agaggcaccc aaatcccaga
     4021 atgcaccagg ggtcgattaa ggccccgcat tcaatcccgc ggtgaatcag ctcaatgaca
     4081 ccggagagcg tcttgagggc ctctgttcgc cggttgttgc gaatcaacca ttcacactcg
     4141 ccaagctgac aacgaatctc gcagcgaatc cgtgccgcgc ccgaatggat ttgttgcatc
     4201 cagtaacggg cctcttcgac agcccccatc ttggcaaagc aggtggccaa cgagacgacg
     4261 tacaactctt gagagcgacg agccgcaagt ttttgattga gcgcctggcg ggtggcccca
     4321 aatggttgac gcaggcgttg catctcagac tccagccgct gccgtctctc tccagtacat
     4381 cgcccgagaa gctcgatata aaactgatcc cgcacaaggg caattccctg aagccatttg
     4441 tcgatctgga cactggagag aatggccccc gggcggtctc cggagattcc cgaagccatc
     4501 agaattgtcc ctgccaggac cgctgcccct tcgaagaggc gctcttcata atcatccgca
     4561 ggtccttcca acacacgggc aaggatactg ttcaaatgaa catcatgaag gacaaaccga
     4621 cagtagtagc cacggtcatt gagtttccgc ggatcccata gcccatagag gtaattgggc
     4681 cgctggtgca cgggatgttc catatcataa ccgcggggat cgaggacgat ttcctccagt
     4741 tgctcgaagt caaaataact cgcccggcaa agctccgccg atgtctcgcg gagaattccc
     4801 agcgctgtct caatcaatcg ttgatacggg cctacggcgg cccctgcgcc acggatccaa
     4861 aggggaatcg gacagacaaa ctcgtgctcg tatggtttgt gacgtttttt tccctcgagc
     4921 gtgggcaccg gccgatagcc caaatagtca ttcagccgcc tgatggtggc ttcaagcatc
     4981 tcgttatcat cggcctttcc gtcaagctcg gcctcgtgca gctccgctaa gatggcttca
     5041 caaaaccgtg cgagcagaaa aggttggcaa tagtcgtcga gggtaagatg agccaagata
     5101 tcgcggtgaa actgccggta gccgtgaatc aggcgttcaa agagaaattc taggacatac
     5161 tctgcctggg ccgtatcgga aaaagcagga ttttcgcgcc gcagtcggct cagttcgtta
     5221 tcaagatacc gatgcagcaa ctccggcctc tcttgagttt gagcctctga gggttgcgaa
     5281 aggaacgcaa tttcccggag tgtccagagg cgattccacg cagcaaagaa ccgcggatct
     5341 cggaccccct gagagaaatt caggtaaccg agaatcacct ctaccggaga ggctgacttt
     5401 tctggcttca ttcaacacca gctccttgat aatccttccc agcctaaatc catccaccca
     5461 tctcaagtcg agcatccagc ccgactaggg tgtggcattt cactcagcgc tcaagttcat
     5521 ttcaggcacc ctgaacccct tttgccatgg cgtgctaaca cgtgcacagc gtatccaagc
     5581 cacgggcaca acttgccctc tcacccaaag cagcgtcggc ggcgcacacg cggccactca
     5641 acctattccc gcattttacc gcacggcggc ggtttggata acctgagcga gagcttggct
     5701 gaaactccgt ggtcttcggg aacactcatg gagcgaaatc ttttagattt tcttcccccg
     5761 gcgggcaaag gcccagtcaa gcgttgggca gacggtgccc aatttgttcg gtcacc
//
LOCUS       DAOPTR010000081         5808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_11271_length_5808_cov_3.527377, whole genome shotgun sequence.
ACCESSION   DAOPTR010000081 DAOPTR010000000
VERSION     DAOPTR010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11271_length_5808_cov_3.527377"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="PKI05_02620"
     CDS             <1..1190
                     /locus_tag="PKI05_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51121.1"
                     /translation="EDPAAIPLGAANLCPDNRTASVGHLNSGEDAYLLVRTAAEQSSW
                     IMVNLYEERASNTHIFWDSLENDAFSLLWENDPIEGVTARVTINSVDISFALQEAAET
                     QIVFHSQWGIDTMTIEGTPADDSLQGWLDRVVFFPGGFGEGETGHNVRVEVNGFDFLH
                     AYGRGGNDRASIYDQTSTAEATAKIKLKSEPQFNHVKVIGPQTFIRVKLFETVEVFAD
                     GAGDQALFYDSTGDDFFIGFLGYSQMTGPGYEVKAYGFPFVTAYSKNGGNDQVRFVDS
                     VMKDEFSLKPHKSEVFDQVTGGAVYRIVARGFDRVQVEAPSATGGRDKAAIWDTIFSD
                     NVEVFGDRLTFTRTVSRPEVIFDIRGVEFVKIRPSSGGDDRVLIRIPPEIELVIGDGW
                     QIL"
     gene            complement(1231..3567)
                     /locus_tag="PKI05_02625"
     CDS             complement(1231..3567)
                     /locus_tag="PKI05_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51122.1"
                     /translation="MWAFVHSLMERYSGRTWQLVTLISGLVLLLAGLATARFGLSSGS
                     PVVRAAEPHPAQPPMDPERSPTPAPLPDAEKAVPLSAQAPSKGAAIRLSTSGRTQDTI
                     PPEPRPTLAGGIPSAANVAWNDVNTPMPGESATVEAHNGESIPSGSPWVVPPMPDAGA
                     VQAGQNQSPAPRTELQRPTFAGQPRPNERPAGQDVADGADTLRNPPSETPPAESNPVS
                     GGQSAPPQPLASSQGEITSLGPTQSGNAAPGAPAATPSSPFDALPPPSSGTRQAIEGT
                     TGGLSSIGAPAPIPAATAGAANPLVMADGSGVGTEGDARPGDPALEGPQSPQVSIHKL
                     MPEEVQVGKPALFRIVVRNSGAITIPKVEVHDPIPWGVRVLRTNPSATQGSQGTLVWT
                     LGPLHPGDEAVLEAEVMPLQEGELGSVTTVLVHTQASARTKCTRPQLTVKTQAQPQVM
                     IGDEVLVTMTVSNTGSGVATNVVLEEHVPPELQHPAGNELENVLGDLKPGESRQVQLK
                     LQAIRAGNAVNRLIARADGNLRVEDQTTIQVLAPQLEVTVGGPSRRFLDRETSYQIAL
                     VNRGTAPAKNVRLTASLAAGLQFVRTNNAGAYDPTTHSVQWLLAELPVGQTGMVELVV
                     NPIAAGQQELVCSVRDELGISSESRLSVLVEGISALHFQVADTKDPIAVGMETTYEIR
                     VVNQGTKEATNVQVMVTFPEGIRPLAGEGPVPNTVNGNEVLFGKLNRLAPRGEAIFRI
                     RSQAVGPGDQRIRVQILSDDIRLPITKEESTQVYSDSE"
     gene            4365..5786
                     /locus_tag="PKI05_02630"
     CDS             4365..5786
                     /locus_tag="PKI05_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51123.1"
                     /translation="MARENQALQIALITFVILTLLLGVMTYLFFRQYDEQFRKTQTAL
                     EEAAKANDAARSAQTDLNTLKTLVGFATTEPMDRISQQAKQELETYGSDLDPSQQNYR
                     AVLDHLFKTLQAKSATLVATEQKLRDAETKIAQLEASKAPLIKQQQDRADTSEATLAQ
                     ERQKFTTDLQASFKARDDLVNQVTEARQQAQDAVAQMEAAKKDFETRMRTASALLVAR
                     TRELEATKNPRLETPDGKIQWVNQALGTVWINLGEADGVSIGTMFAVFPGDTVNVADA
                     ELKGGIEVTAIRGPHLAEARIIDDRIGNPIMPGDVISSLIWSPGDRRRFALTNFIDID
                     DDGRSDNELLISIIRSSNGIVDAWLDDDGTLHGSITADTDYIVVGDEPPENASVTAIQ
                     ARSDLLKRADEMAVLRISVRDLLRRIGYKRETHTVRFGAGANPADFRAKMPEDQVIRR
                     SGGAVSEVFQPRQPPRAKAGTAF"
BASE COUNT         1203 a         1592 c         1760 g         1253 t
ORIGIN      
        1 tggaagaccc tgcggcgatc cccctggggg cggccaatct ttgtcccgac aaccgaacgg
       61 cttcagtggg tcacttaaac tcaggagaag atgcctatct tcttgtcagg accgccgctg
      121 aacagtcttc gtggatcatg gtcaatctct atgaggagag agcttcaaat acccatatct
      181 tttgggacag tctagagaac gatgccttca gcctcttgtg ggaaaatgat cctattgagg
      241 gcgtcaccgc gcgggtcacg atcaacagtg tggatatttc ctttgcctta caggaagccg
      301 cagagacgca gattgtcttc cattcccagt ggggtataga cacaatgacc atcgagggca
      361 cgccagccga tgattcctta caggggtggc ttgaccgggt ggtgtttttc cccgggggat
      421 tcggtgaagg agagacgggc cacaatgtgc gggtggaggt gaacggtttc gactttctcc
      481 acgcctatgg ccgaggcggc aacgaccggg caagtattta tgaccagacc tcaacggcgg
      541 aagcgacggc caagattaaa ctcaaaagtg aaccccaatt caatcacgtc aaggtgattg
      601 gcccgcagac gtttatccgc gtcaagttgt ttgagaccgt tgaggtcttt gccgatggag
      661 cgggggatca ggccctattt tacgatagta ccggcgatga ttttttcatc gggttcttgg
      721 gttatagcca aatgacgggt cccggatacg aggtaaaggc gtatggtttt cctttcgtca
      781 cggcgtattc caagaatggc ggcaacgatc aggtaaggtt tgttgattcg gtgatgaagg
      841 acgagttttc cctgaagcct cacaagtcgg aggtgtttga tcaggtcacg ggcggggccg
      901 tttatcgcat cgttgcccgg ggattcgacc gtgtgcaggt ggaggcgccc tcggctaccg
      961 gtggacgtga taaagccgcc atttgggaca cgatctttag cgacaacgtt gaggttttcg
     1021 gcgataggtt gacgttcacc cgcacggtat cccgcccgga ggtgattttt gatatccgcg
     1081 gtgttgaatt cgtcaagatc aggccttcca gcgggggcga tgatcgcgtc ttgatccgca
     1141 tcccgccgga gattgaactc gtcattggcg acggctggca aatcttatag ccgtgcaaac
     1201 gcggcgtttt ggtcaagatg ctccgttggc ctactcgctg tcggaataga cctgtgtgct
     1261 ctcttccttg gtaatgggca agcggatatc gtcgctgagg atttgcacac ggatgcgttg
     1321 atcaccgggg ccaactgcct gcgaacggat gcggaagatg gcctcgcccc tgggggccag
     1381 tcggttgagt tttccaaaga ggacttcatt accgttcacc gtgttgggga cagggccttc
     1441 gccggcgagg ggccggattc cttcggggaa tgtgaccatg acctgcacgt tggtggcctc
     1501 tttcgtcccc tggttgacaa cccgaatttc gtaggttgtt tccatgccta cggcgatcgg
     1561 gtcctttgtg tcggccactt ggaagtgaag ggccgaaata ccctccacca gaacggaaag
     1621 tcgcgattcg ctgctgatgc ccaattcgtc ccgcaccgag cagacaagtt cttgctggcc
     1681 tgcggcgatg gggttcacca ccaattccac cattcccgtt tgccctacgg gcagctcggc
     1741 cagtagccac tgaacgctgt gcgttgtggg atcgtaggcc ccggcattat tcgtccgcac
     1801 aaattgcaaa ccggcagcta gtgaagccgt caggcggacg ttcttggccg gtgccgtgcc
     1861 ccggttgacc aacgcaatct ggtagctagt ttcccgatcg agaaatctcc gcgagggacc
     1921 acccacggtg acttccaact gaggtgccaa gacttggatc gttgtttgat cctcgacccg
     1981 caaattaccg tcagcccgtg cgattagccg gttaacggcg ttgcctgcgc ggatggcttg
     2041 gagtttgagt tgcacctgcc gggattcgcc gggtttcaaa tcgccgagga cattttccaa
     2101 ttcgttcccc gctgggtgct gcaattccgg cgggacgtgc tcttccaaaa cgacattggt
     2161 ggcgacgcct gatcccgtgt tgctgaccgt catcgtcacg agaacttcgt cgccgatcat
     2221 gacctgcggt tgggcctgcg ttttgaccgt caactgtggc cgagtgcatt tggttcgcgc
     2281 cgatgcctga gtgtggacca gcaccgtggt gacgctgccc agttccccct cttgcagagg
     2341 catgacctcg gcttccagaa cagcctcatc gccaggatgg agggggccaa gggtccagac
     2401 caacgtccct tgggagcctt gggtggcact ggggttggta cgaagcacgc gaactcccca
     2461 gggaatcggg tcgtggacct caactttcgg tatggtgatg gccccactgt ttctcaccac
     2521 aatgcgaaac agggccggtt ttcccacctg aacctcttcc ggcatcagct tgtggatgga
     2581 cacctggggt gattgcggcc cttcgagtgc gggatccccc gggcgggcat caccctcagt
     2641 tcccacgccc gaaccgtcgg ccatcacaag gggattcgcg gcaccggccg tggctgccgg
     2701 gatgggagcg ggggcaccga tacttgagag gccaccggtg gttccctcga tcgcctgccg
     2761 tgtgccggag gaaggtggtg gaagggcgtc aaacgggcta cttggagtcg cagccggagc
     2821 accaggtgcc gcgttacccg attgcgtcgg gccaagtgaa gtgatctcgc cttgtgagga
     2881 ggcgagcggt tgcgggggtg ccgattgacc accggaaaca ggatttgatt ccgccggagg
     2941 tgtttcgcta ggcgggtttc tcaacgtgtc cgcaccgtct gccacatctt ggccagcggg
     3001 acgttcattg ggacgcggct gtcccgcaaa ggtgggccgc tggagttctg tccttggggc
     3061 gggcgactga ttttgcccag cttggacagc gccggcatct ggcatgggcg gtacaaccca
     3121 agggctcccg gagggaatag attcgccgtt gtgggcctcg acggtggccg attcgcctgg
     3181 cataggagta ttcacgtcgt tccatgccac gttcgccgca gagggtattc cgccggccaa
     3241 tgtgggacgg ggttccggag gaatggtgtc ttgagtgcgg cctgaagtgg aaagccggat
     3301 agccgctccc ttggacggcg cctgtgccga gagggggacc gccttttccg cgtcggggag
     3361 cggtgccggc gttggagaac gctcggggtc catgggcggc tgcgcgggat ggggttcggc
     3421 tgcccgcacc accgggctgc cactcgatag gccaaaccga gccgtcgcta accccgccaa
     3481 cagaaggacc aacccggaaa tcaaggttac aagttgccag gttctccctg aatatcgctc
     3541 catcaaggaa tgaacgaacg cccacacgga acgaccctcc atagtctccc aaagatatcc
     3601 gtttacggtg tgaatcggag aacaaaagcg ccccggacct tgagaagcac aaggacctag
     3661 gcatgtgctt aattaccaaa aatgagcgcg ataggaaaga ccaaccgacc ccgctgtctg
     3721 ggtggataaa acccgccgat atcacgatcc gccattgtga actaacggcc tgcgaccccg
     3781 ccacctaacg atggcggctt cggcatatat ggagcctatg ccaaaattcc ctctccctct
     3841 ggtagagggt tggggtgagg gtaagccatc gtcggagtcg ttcgagaaat tcagcagcga
     3901 tctgtgccac cctcaccccc ggccctctct cgtttcggcg ggagagggga ggtcgtgtga
     3961 taggctctta cctgcggcca cgttggcgcg tcggcttcca tcactcctcc caggccgaac
     4021 gtgtaaaccg gcaggtggaa atatctactt tcaattcctt gaacgggcag gcacggggac
     4081 ctggcggaat gggcttggaa aacacacagt cgatccgcaa acgccttcat cccgcaacgg
     4141 ggtctccgat attgctggcg tttgattgac attggtgggg cggctattta gaatcgacgt
     4201 agatttatgt tgcccagatt gccaggtttt tcaaaacgtg agacttgaaa tacattcgag
     4261 ttcgcgttga cggggcaagg gttccggaac catcgttgtt gggcgagtag cattggggct
     4321 ggagatcgac tgacccatcg aggacgctca ggagcaaact tgtcatggca cgtgagaacc
     4381 aagcgctcca aatcgctttg atcacattcg tgattttgac acttctgttg ggcgtgatga
     4441 cctatctctt cttccggcag tacgacgagc agttcaggaa aacgcaaacg gccttggaag
     4501 aggcggccaa ggctaacgat gccgcgcgtt cagcccagac cgatcttaac acactcaaaa
     4561 ctttggttgg ttttgcaacc accgaaccga tggatcggat cagccagcag gccaagcagg
     4621 aattggagac gtacgggagc gatcttgacc cgtctcagca gaattaccgt gccgtccttg
     4681 atcacctgtt caagacatta caggccaaga gtgccacttt ggttgccacc gagcaaaagc
     4741 tccgagatgc ggaaacaaag atcgcgcaat tggaagccag caaagcccca ttgatcaagc
     4801 aacaacaaga tcgggctgac acctccgagg ctacgttggc ccaggaacga cagaaattca
     4861 ctaccgattt gcaggcatca ttcaaagccc gcgatgactt ggtgaaccag gtgaccgagg
     4921 cccgccaaca ggcccaggat gccgtagccc aaatggaagc cgccaagaaa gatttcgaga
     4981 cccggatgcg gactgcctct gccttgctcg tggcgcggac tcgggaattg gaggctacca
     5041 agaatccccg cctggagacc ccagatggca agattcaatg ggtcaatcaa gcattgggga
     5101 cggtttggat caacttgggt gaggcagatg gggtctcaat cggtaccatg tttgccgtat
     5161 ttcccggaga tacggttaac gtggcagatg cggaacttaa aggcggtatt gaagtcacgg
     5221 ccatccgtgg tcctcacctt gcagaagccc gcatcattga tgacaggatc ggcaatccca
     5281 ttatgccggg tgacgtgatt agtagcctca tctggtcacc gggcgatcgc cgtcgttttg
     5341 ctttgacgaa tttcatcgac atcgatgacg acggccgaag tgacaacgag ctgttgatca
     5401 gtattattcg tagcagtaat ggcatcgtgg atgcctggct ggatgatgat ggcacgctcc
     5461 acggcagtat cacggctgac accgattata tcgtagttgg cgatgagccg ccggaaaatg
     5521 cttctgtaac tgccatccag gcccgttccg atttgctcaa gcgggcggat gaaatggcgg
     5581 tcttgcgaat ttccgtccgc gacctcttgc ggcggatcgg ttacaaacgt gaaacgcaca
     5641 cggtccgctt tggtgcgggg gccaatcccg cagacttccg ggcgaagatg cccgaggatc
     5701 aagtgatccg gcggtccggc ggtgcggtga gcgaagtctt ccagccgcgt cagcccccgc
     5761 gggccaaggc cggaacggcc ttttgatacc catcccgcaa atagctaa
//
LOCUS       DAOPTR010000082         5808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_11272_length_5808_cov_3.182513, whole genome shotgun sequence.
ACCESSION   DAOPTR010000082 DAOPTR010000000
VERSION     DAOPTR010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11272_length_5808_cov_3.182513"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PKI05_02635"
     CDS             complement(<1..730)
                     /locus_tag="PKI05_02635"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOA51124.1"
                     /translation="MSRARQTGFEHCALVSGLVTQEQLDEAWQRLRAKPSSQLEDVPI
                     EERLAQQLVELGYLNNWQAKQLLEGRTKFTLGPYKIIDSIGRGGMGQVFKGEHSLLGR
                     IVAVKVLPRHKSSPEAVENFLREIRALARLDHPNLVRAYDAGLDGNVVYLVTEYVPGS
                     DLRKLIRQRGPLHMHAAASIISQVASALEHAHSQGLIHRDVKPGNILVTNDGHAKLLD
                     LGLAGPLGQEAVNDPRYGRIVGTVD"
     gene            complement(927..1565)
                     /gene="hisH"
                     /locus_tag="PKI05_02640"
     CDS             complement(927..1565)
                     /gene="hisH"
                     /locus_tag="PKI05_02640"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOA51125.1"
                     /translation="MTNTPHEQSDVTIVDYGMGNLRSVQKAFERLGLRAEITDVPDRI
                     LIAERLVVPGVGAFGDAIQELERRGLVKPIMKFIESGRPFLGICLGLQLLFEKSYGNG
                     VHKGLGLLRGDVVRFELPSEFSIPHMGWNQVRQECPTPLMRDIPDESYFYFVHSYYVV
                     PTDPEVVAGTTDYGVRFCSMIRQDNVFATQFHPEKSQSLGLQLLRNFASLVS"
     gene            complement(1590..2486)
                     /locus_tag="PKI05_02645"
     CDS             complement(1590..2486)
                     /locus_tag="PKI05_02645"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOA51126.1"
                     /translation="MVLRILQSLFSGSKSRIGPAKKKVDVAARFQILREGVAGTMSRF
                     YVAMDRERGEIVGLKILDPQKTAAYEARFKGLNKPCEGEIAMRFVHPYIVMTYEYGWT
                     TRDEPYLVMEYLEGGGLNGHLVAKSPFLFKKELLPYIRQVAEALAVVHEGGFIHRDVC
                     PRNLMFNRDFSILKLTDFGLSVPATPPFMQPGNRSGTPNYMAPELVRRYPTDQRLDVF
                     SFGVSMYEMLTFELPWERGTTGLAALQHEKPPADIRRFRPQIHPDLAEAIHACIEPVK
                     EKRCPSMRHFLEMIEHLQSTDL"
     gene            complement(2701..3576)
                     /gene="accD"
                     /locus_tag="PKI05_02650"
     CDS             complement(2701..3576)
                     /gene="accD"
                     /locus_tag="PKI05_02650"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOA51127.1"
                     /translation="MADNPLASASETSRETNGWQNYVKRPKRGVPAGLWLRCPGCGAT
                     VFRKDVEKLLGVCPECDYHWYVSAHERLKQLLDEGTFEEWFANLESCDPLEFVDKISY
                     RERLKRERERTGLKDAVIAGTGMIRARRVAIGVTDSAFMMGSMGSVVGEKLTRLIERA
                     QKEQLPLIIVSGSGGGARMQEGVFSLMQMAKVSAALARFHASGGLYISVLTNPTMGGV
                     AASFASLGDVILAEPKALIGFAGPRTIMATIRIELPKGFQTSEFLLEHGFIDRIVRRP
                     ELKTEIARIIDYCGK"
     gene            complement(4091..>5808)
                     /locus_tag="PKI05_02655"
     CDS             complement(4091..>5808)
                     /locus_tag="PKI05_02655"
                     /inference="COORDINATES: protein motif:HMM:NF028290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOA51128.1"
                     /translation="NRKLVIALVVVFLGYGLLLALGWPQAATRMIVEAESHASASAHH
                     DSGADQHKAPAHSEESASETTSPAEDGHAEEERSKAVEDEQAGADPTGRASSEEAHAG
                     HSEGAEATSHSVTTHPPLWMVVPFVALLLIIAVFPLLPQTEHWWESNLHRFYVAAALA
                     AVTLLYYLVVYEHPVEGHWPAHYVSQPAAGLNWGNAWAVFANAILSEFIPFIVLLFSL
                     YTISGGIRIEGDLRATPLVNSIIIFVGAVLASFVGTTGAAMLLIRLLLDTNSERKHVA
                     HTVVFFIFAVCNCGGCLLPTGDPPLFLGYLRGVPFLWTLQLWKEWAFVNIALIVIYFL
                     WDTFWFYPHEQKRDLRRDAAEVRRLRVRGLWPNLPLMIGVVLGVALLDPSKPFPGTEI
                     HPPLYLREIFQLGMVALSLTFGSYENRVANRFNYAAILEVAALFFGIFITMQPPLQIL
                     HVEGPRLGLTQPWQFFWATGALGSFLDNAPTYVVFFETARTLGGHDLVPPGTGVAHDL
                     LVAVSLGAVFMGANTYIGNGPNFMVRAIAEKRGIKMPSFFGYMVYSGCILLPLFVLVT
                     IVFLS"
BASE COUNT         1412 a         1728 c         1464 g         1204 t
ORIGIN      
        1 aatccacagt tcccacaatc cgtccatagc gagggtcgtt cacggcttct tgtcccaggg
       61 gccccgctaa gcccaaatct agcagcttgg catggccatc attggtgacg aggatatttc
      121 ccggtttgac atcccgatgg atcaatccct ggctgtgagc atgctccaac gctgacgcca
      181 cctgcgaaat gatggacgca gcagcgtgca tatgcaaagg acctcgctga cgaatcagct
      241 ttcgcagatc ggatccgggg acgtactccg tcacgaggta aacgacgttt ccatccaggc
      301 ctgcatcata ggcccgaacc aggttgggat gatccaatcg cgctagggcc ctaatctcgc
      361 gcaaaaagtt ttccacagcc tccgggcttg acttgtggcg gggtagaacc ttgacggcaa
      421 caatccgtcc caggagggag tgctcgcctt tgaagacctg ccccatcccc ccgcgcccga
      481 tggaatcaat aatcttgtat ggacctaacg tgaactttgt ccgcccctcg agaagctgct
      541 tcgcctgcca attattgaga tatcccaact caacaagctg ctgagcgaga cgttcctcga
      601 tgggcacatc ttccaattgt gacgacggct tggcgcgcaa ccgctgccaa gcctcgtcaa
      661 gctgctcttg cgtaaccaag ccactgacga gcgcgcagtg ctcaaaacca gtctgcctgg
      721 cgcggctcat accataggag ctccacccaa gtcggacact tgccctcttc aagattatag
      781 cgtctcccca cagaagaacc ccaacagtga cacgccaaga cgcggcgctg tacacacacc
      841 accctcaacg ctgcgcccta ttgggtaccc aatgtcaccc atccttcagc aagaggagcg
      901 ccgaatatta gctacccctg ggacgctcaa gacacgagac tcgcgaagtt tcgcaacagt
      961 tgcaagccca atgattgact cttttcaggg tggaattgcg tggcaaacac gttatcctgt
     1021 cttatcatgg agcagaatcg cacaccatag tcggtcgttc ccgccacaac ttcaggatcg
     1081 gtgggaacca cgtagtagga atggacaaaa tagaagtagc tttcgtcggg aatatcccgc
     1141 attagtgggg tagggcattc ttggcgaacc tggttccagc ccatatgggg gatagagaac
     1201 tcggaaggca actcaaatcg cacgacgtca ccccttaaaa ggccaagccc tttgtggacg
     1261 ccgtttccgt aacttttctc aaaaaggagt tgtaacccta agcaaatccc aagaaacgga
     1321 cgtcccgact caataaactt catgatgggc ttcactagcc ccctgcgctc aagctcctgg
     1381 atggcgtcac caaaagcccc cactcccggc actaccaagc gctccgcgat caagattcgg
     1441 tccggcacat ccgtgatctc cgcacgcaga cccaatcgct caaaagcctt ttgcacactc
     1501 cgcaaattgc ccatcccata atcaacgatc gttacgtcgc tttgttcatg aggagtgttc
     1561 gtcatggcta ttcaaaggct tccaactcgt tacaagtcgg ttgattgtag gtgctcgatc
     1621 atctcgagaa agtgccgcat ggaaggacac cgcttctctt ttacaggttc aatgcatgcg
     1681 tgaatagcct cagccaggtc gggatgaatc tgtggccgaa accgccggat gtcggccggt
     1741 ggcttttcat gctggagggc tgccaaaccc gtcgttcccc gctcccaagg cagctcaaag
     1801 gtcaacatct cgtacatact gacaccaaaa gagaatacat ccagtcgttg atccgtggga
     1861 taacgacgca cgagctcggg cgccatgtaa ttgggtgtgc cgctgcgatt cccgggctgc
     1921 atgaatggcg gcgtggccgg aacggacaga ccaaagtcgg tcagtttaag gatcgaaaaa
     1981 tcacgattga acatcaggtt gcgagggcac acgtctcggt gaataaatcc gccttcatgg
     2041 actaccgcta gggcctccgc cacctgccga atgtagggaa gaagctcttt tttgaacaag
     2101 aagggacttt tcgccacaag gtggccatta agaccgccac cttccaagta ctccatgacg
     2161 aggtacggct catcccgcgt tgtccatccg tactcgtaag tcatcacaat gtagggatga
     2221 acaaaacgca tggcgatctc gccttcgcat ggtttgttta accccttgaa acgcgcttcg
     2281 tacgctgccg ttttttgggg gtccaggatc ttgagcccga caatttcgcc gcgttcacgg
     2341 tccatggcta catagaacct ggacattgtc ccggctaccc cttcccgcag aatctggaaa
     2401 cgcgctgcaa catccacctt tttcttggcc ggccctatcc tcgatttcga accggagaac
     2461 aggctttgca gtattctcaa caccatggag aaagtgccca atcctagccg tcgtacacat
     2521 cagccaaagc cacactcgtc gaagtacgcc ctttgggtaa gtttagacgc gataagggcg
     2581 ccattcaaga aaagcgaaac gcccggccaa attctcacag ttaatcgcgc attggcaggc
     2641 cacatccccg cccgatgaaa catcccccag ggttcgcgct gcccacttgc ggtcacggcc
     2701 ttatttcccg caatagtcga tgattcgggc aatctccgtc tttagctcgg gccgacgcac
     2761 aatccggtcg ataaaaccgt gttccaacag aaattcgctg gtttgaaatc cttttggcaa
     2821 ctcgatgcga atcgttgcca tgatcgtccg gggacctgcg aaaccgataa gggccttggg
     2881 ctccgcaaga atcacgtcac caagcgaagc aaaactggcg gcgacccctc ccatggtggg
     2941 attcgtcaag accgagatat aaagccctcc ggaggcatga aagcgagcca acgccgccga
     3001 gacttttgcc atttgcatca gtgaaaacac gccttcctgc atccgcgccc cgcccccaga
     3061 gccggaaaca atgatcaagg gaagctgctc tttttgggcc cgctcgatga gccgggtcag
     3121 tttctcgcca acgaccgagc ccatgctccc catcatgaag gctgaatcgg tcacgccgat
     3181 agccacccgt cgggccctaa tcatgcccgt gccggcgatg accgcatctt tcagaccagt
     3241 acgctcccgt tctcgcttca gccgttcccg ataggagatt ttatcaacaa actccagagg
     3301 gtcgcaggac tccagattgg caaaccattc ttcaaacgtg ccttcgtcaa ggagttgctt
     3361 gagccgctca tgcgcagata cgtaccaatg atagtcgcac tccggacata ccccaaggag
     3421 tttttcgaca tctttgcgga agacggtggc gccgcagccg gggcacctca accagagccc
     3481 agcaggcacc ccccgttttg ggcgcttgac gtaattttgc caaccgttgg tctctcgcga
     3541 cgtctctgat gcagaagcaa gcggattatc agccataaag atgctgcctt tatgctagga
     3601 tagcaatgac cgtccaagtc agtatctctt taaggcaatc ggttgttcgc cgggctttct
     3661 taaagaatcc aagtcagccc tagaaagggc agtatgccca ggttgccacc gtcttgcaac
     3721 ccgtcatcct agtggtatgg cataccccac tatcgggcag cctgaggcaa ttgcattgcc
     3781 ggtatcgttc cattaagcgg ttctctttgt caagaagggg cactcggcgg gtcccatgca
     3841 gcctccttcg tcttctatct cgaactgcta actgtctcgt gcccagtttt gcactcgcca
     3901 ctcctgtgca tgtgggcgag tgctgcaaca ggatttttcc ctgaatgcgg catatttctc
     3961 tggcaatgat agcaataaca tcgactagga aggccatatc accaaaacaa aacagcgcgg
     4021 ctaacaccgc gctgcaatgt ttcaacgtca ccgacagcag tgccaccggt tgggcttaaa
     4081 cgtccctgta tcaactcaga aagacgattg tgaccagcac aaaaagcggt aacagaatgc
     4141 agccgctata aaccatgtag ccaaagaaac tgggcatctt gatgccgcgt ttttccgcaa
     4201 tggcccgcac cataaagttt ggaccattac cgatgtatgt gttagctccc atgaaaaccg
     4261 ctccgaggct gacggccacc agcaggtcat gggccacgcc cgtccccggc ggcaccaagt
     4321 catgtccccc caatgtcctg gctgtttcaa agaagacgac gtatgtaggg gcgttatcga
     4381 gaaagctacc tagtgcgccg gtggcccaaa agaattgcca cggctgggtt agccctaatc
     4441 ggggcccctc cacatggagg atttgaagcg gcggttgcat cgtgataaag atgccgaaaa
     4501 acagcgcagc gacctccaag atagccgcat agttaaagcg attggccaca cgattctcat
     4561 agcttccaaa cgtcagcgag agcgcaacca ttcccagttg gaagatctcc cgcaagtaca
     4621 aaggtggatg aatttccgtg ccgggaaacg gcttgctcgg atcaagcagg gctactccaa
     4681 gtaccacccc gatcattaag gggagattgg gccaaaggcc ccgcaccctt agccgtcgga
     4741 cctccgcagc atcgcgtcga aggtctcgct tctgctcatg cgggtagaac cagaacgtgt
     4801 cccacaagaa gtagatcaca atcagtgcaa tgtttacaaa cgcccattct ttccagagtt
     4861 gcaatgtcca aagaaagggt accccgcgta ggtaacccag aaacagcggt gggtcaccag
     4921 tggggagcag acatccccca caattgcaga ccgcgaaaat aaagaatacc accgtgtggg
     4981 ccacgtgctt ccgttcgcta tttgtgtcca ggagcaaccg aatgagcaac atggctgccc
     5041 cggtcgtgcc tacgaagctc gccaacacag cacccacaaa gatgatgata gagttcacaa
     5101 ggggcgtcgc ccgcagatca ccttcaatgc ggataccacc gctaatggtg tagaggctaa
     5161 acagaagcac aatgaagggg atgaactcgc tgaggatcgc attggcaaaa acagcccagg
     5221 cattgcccca gttaagaccg gcagccggct ggctaacata atgggcaggc caatgccctt
     5281 ccacggggtg ctcataaacg accagatagt aaagtagcgt aacagcggcc agtgccgccg
     5341 caacgtagaa gcgatggaga ttactttccc accaatgctc agtttgcggc aatagaggaa
     5401 aaacggcaat gatcaacaaa agtgcgacga agggcacaac catccaaagc ggcggatgag
     5461 tggtcacgct gtgggaggtc gcctctgcgc cttccgaatg tcccgcgtga gcttcctcag
     5521 aagatgcccg ccccgtcggg tcggccccag cttgctcgtc ttccacggcc tttgaccgct
     5581 cctcttcagc atgcccatcc tctgccgggc tcgtcgtttc tgaggccgac tcctctgaat
     5641 gagctggggc tttgtgctga tcagccccgc tatcatggtg agcggaggcc gaggcatggg
     5701 actccgcttc cacaatcatt cgcgtcgccg cctgcggcca gcccaatgcc aataacaggc
     5761 cgtagccgag aaagacaacg actaaagcga tgaccagttt tcgattga
//
LOCUS       DAOPTR010000083         5742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_11439_length_5742_cov_2.836117, whole genome shotgun sequence.
ACCESSION   DAOPTR010000083 DAOPTR010000000
VERSION     DAOPTR010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11439_length_5742_cov_2.836117"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1756
                     /locus_tag="PKI05_02660"
     CDS             <1..1756
                     /locus_tag="PKI05_02660"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOA51129.1"
                     /translation="ETAVLVNAIVDAYMREVVDTEQHERRVRLSELDQLYAEKEAELR
                     KLQNDLKTLSQQLETGDSQALSLKQQITLQQFSTYRSELINVQLRIMRAQGELSLKKA
                     IREKAAQIEITERELDAAAKTDPITLQLLMREAELKNLVAQTRDVVRPEMAGRFGNRY
                     VQDLADVQARIKARREELRQELEQKRVASIDQEIENLNFELAILNQQQQQLQQEVENV
                     RRVAETFGKGSIEVEFLRAEVELARKTVQTIAEERNRMSIELRAKPRITVVQRAEAPR
                     VADQDRRVQLSALAGLAGLVLPGITLLWWDTRRKPVNTPQEIRHVAGAEVWGTIPRLP
                     VRVMRAIGNGRVRSKKHRRWQRMFVEAVRIVAARLVRESEDERLKVIMVTSAVAGEGK
                     TTLAAQLALSLAQIGKKTLVADFDLRRPAIHDVFDVPISPGVSEVLRGEVSLQDAVRA
                     TSVENLYVLPAGYWSPHRLAVLANGVVGATFQQLREDFDFIIVDGTPVLPVADTRFIS
                     THTDGVVLSVIRDYSRTPLVRSAKQILESFRVRVLGTVVTGSTEEVYHSYSYHDLVDG
                     TSSALSDGEEVESEKADT"
     gene            2017..3705
                     /locus_tag="PKI05_02665"
     CDS             2017..3705
                     /locus_tag="PKI05_02665"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOA51130.1"
                     /translation="MTQPMAVYAAVSCLAAMIASAVFTRFMCWVAPKIGFVDQPDRNR
                     KVHKKATPLGGGLAIYLAICLVLGVNICLANPWGLRVHQDWWDLLVLFIAGSWIVGLG
                     LIDDWSRLRGRYKLAGQFVAAAILVAGGYTFSSFRLFGISVELGWIGAVVALFWFLAT
                     INSINLLDGMDGQAATLGTIFSITVGIMACVTGHYAVAFVAFVLAGATFGFLWFNLPP
                     ARIFLGDAGSMLIGLMLGVLAARASLKGPSTFLLAAAVALWTLPFLDCFAAIIRRTLS
                     GRSIYAADRSHIHHCLMEKCKSSETALMVISACAAVTAVASLGGLVLGNDTIALIAAS
                     GVVAFLIVTGLFGRSECRLILNTLRRWGDGLRRWLLGQEGKCLCNGTHFQGKFDWHKL
                     WTHLTDPAGEYNVIYLRVDLNDPRIQEGYCGEWKGPVFPNPDDTPISHHEFPLCTGDR
                     VVGKIKAVLRRSAETFLRDLEFVISLVDPFEQQLEQLNDERASQERADKEHSVGTHGS
                     HIFKNVLPTNSIKMTIPGNGDGNGKRSLISHGAETTKTPSIRDLSGGKPTESRK"
     gene            3702..4496
                     /locus_tag="PKI05_02670"
     CDS             3702..4496
                     /locus_tag="PKI05_02670"
                     /inference="COORDINATES: protein motif:HMM:NF023410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase-associated EpsI family protein"
                     /protein_id="HOA51131.1"
                     /translation="MRAAGKGSATRHGPPGTTKTGELSGHSTKTSHLRDSATNAAQKR
                     RRGLSLLLRAGLVALGLLVIGIGARWAASQAVPNIVRVPEQEQLAAFPKQLGPWKCED
                     EEKIDAKMFQALGTGVAVTRRYSQSAERFLEMHAAVFTQPGFSLPHPPELCYSGTGWR
                     VRSTRDVPLTLIDGTSGSVRFIRLVKERGDQQATVLYCYQLAGPFSADRDKVRTFFWK
                     FRGQKSRPPLVKLMLHLPGSGETIENLGLDFAQRALEKLAEMEKTW"
     gene            4531..5589
                     /locus_tag="PKI05_02675"
     CDS             4531..5589
                     /locus_tag="PKI05_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA51132.1"
                     /translation="MRTDPIIPDSRPTEAELTADRPPEKAGLGEDGVNVTADLTAEVA
                     DDWQDRDVVADSEAQPAQADSVPPLPKDLPVTIIRPTGGWRALDLREVWRYRELMLTL
                     AWREITIRYKQTFLGAAWAIIQPLMTTGIFSVLFALLLGKGNEPTAGNVPYAVSTFCA
                     MLPWQLFATSLNQSGNILIQYRSMITKIYFPRLILPMAAVLSSLVDFGIAFVALLALM
                     AFYGIFPGWGLLTLPFFVALAVIASLSVGLWLAALNAIYRDFRYVIPFIIQIGMFVSP
                     VVYSMESIQGRLSPWAWTLYSLNPMAGVIEGFRWALLGTSHGPGPLLALSALATTIIL
                     VCGMFYFRRMERLFADVI"
BASE COUNT         1259 a         1534 c         1630 g         1319 t
ORIGIN      
        1 ggagacggcg gtgttggtta acgccatcgt cgatgcgtac atgcgcgagg tggttgacac
       61 agagcagcat gagcgtcgtg tgcgattaag cgaattagac caactgtacg ctgaaaaaga
      121 ggccgaactg cggaagttac aaaatgacct caagaccctt agccagcaat tagaaacggg
      181 ggacagccag gccctcagcc tcaagcagca gattacgctt cagcagttct caacctatcg
      241 tagcgagtta atcaatgtgc aattgcggat catgcgggct cagggagagc tttccctaaa
      301 gaaggcaatc cgcgaaaagg cagctcagat tgagatcacg gagcgcgaac ttgacgctgc
      361 tgcaaaaacc gatcctataa ctctgcaact cctaatgcgg gaagccgagc tcaagaacct
      421 ggtcgcacaa acccgcgatg tggtccgtcc ggaaatggcc gggcgctttg gcaaccgtta
      481 cgtccaggac cttgccgatg tccaagcacg aatcaaggcc cgacgtgaag aactccggca
      541 agaattggaa caaaaacggg tagcgagtat cgatcaagaa atcgaaaacc tcaatttcga
      601 actggccatt cttaaccagc aacagcagca gctccagcaa gaggtcgaaa atgtccgccg
      661 ggttgctgaa acctttggca agggctcgat tgaggtcgag ttcttgcggg ctgaggtcga
      721 actggcccgc aagaccgtcc aaaccatcgc agaagagcga aaccgcatga gtatcgagct
      781 tcgggccaag ccgcggatta cggttgttca aagggcggaa gcacctcggg tggcagacca
      841 agacaggcgt gttcaacttt cagccctggc tggtttggcc ggattggtcc ttcccgggat
      901 cacacttctg tggtgggaca cccgtcgaaa gcctgtcaat acgccgcaag agatccgcca
      961 cgttgcaggg gcggaggtct gggggacgat tcctcgtttg ccagttcggg tgatgcgggc
     1021 catcggcaat gggcgagtgc gttcaaagaa acaccgccgc tggcaacgaa tgtttgtgga
     1081 agctgtgcga attgtggccg cccgattggt ccgcgaatcc gaagacgagc gactaaaggt
     1141 tatcatggtt accagcgccg tggccggtga ggggaagacg actcttgccg cacagctcgc
     1201 cctaagttta gcccaaatcg gcaagaagac cttggtcgcc gatttcgacc tgcgtcggcc
     1261 tgccattcat gatgtgtttg atgtccctat cagccccggt gtcagcgaag tcttacgggg
     1321 agaagtttcg cttcaagatg ccgtccgagc gacctcagtc gaaaacctgt acgtcttgcc
     1381 ggcgggttac tggtcgcccc accggctagc tgtgctcgcc aacggcgttg ttggcgcgac
     1441 attccagcaa ttgcgggagg atttcgattt catcatcgtg gatggaacgc ctgtcctccc
     1501 ggtagccgat acgcgattca tcagtaccca cacagatggc gttgtgttgt cggtcattcg
     1561 ggactacagt cgcacaccgc tcgtgcgttc cgcaaagcag attctggaaa gtttccgggt
     1621 ccgtgtccta ggtaccgtgg tcacgggttc gacagaagag gtctatcact cctactctta
     1681 ccatgatctc gtggacggca cgagtagtgc cctaagcgac ggggaggaag ttgaatctga
     1741 gaaagccgac acttaatacc tcgcggacaa aatgtcggtg cgaaatcgcg ccggtgaaga
     1801 aaaggagtca cgcgggtaac acctaacatg accaattgtc aaacgttggc gagatcgagt
     1861 cgtaaatcaa gagcggtttt cgctgttgat ttcccgtttt taggcaagga acggccacac
     1921 gtccatgaag acgttgtgta aatgcaagat gatttggggc ggggacgtgt tagacttcgc
     1981 aaccatggtg attcaacctc cgagggagac gcatcaatga cgcaaccgat ggccgtttat
     2041 gccgccgtga gctgcctagc ggccatgatt gccagcgccg ttttcacgcg attcatgtgc
     2101 tgggtggccc ccaagatcgg gtttgtcgat cagccagatc gcaatcgcaa agttcacaag
     2161 aaagctacgc cgttgggcgg tggcctcgca atctacttag cgatttgtct tgtccttggg
     2221 gttaatatct gtttggccaa tccgtggggt ttgcgagtcc accaagattg gtgggatctc
     2281 ctggtgctct tcatcgcggg aagttggatt gtcgggctcg ggctgattga cgattggtct
     2341 cggcttcgcg gacgatacaa actggcgggt cagttcgtgg ccgcagcgat tttagtggct
     2401 ggcgggtata cgttcagcag ttttcggctt tttggcatat cagtcgaatt aggttggata
     2461 ggagccgttg tggccctttt ctggttcctc gcgaccatca actcgattaa cctcctggat
     2521 ggaatggatg gtcaggccgc cacgctgggg accatattca gcatcactgt gggaatcatg
     2581 gcttgtgtca ccggacatta cgcggttgct tttgtggcgt tcgttcttgc cggggcaacg
     2641 ttcggcttcc tttggtttaa cctccccccg gcaagaatct ttttgggtga cgctggaagt
     2701 atgttaatcg gcttgatgct cggtgtgctt gctgctcggg cttcgcttaa aggtccgagt
     2761 actttccttt tggcagcggc agtcgccctc tggacgcttc cgttcctcga ctgctttgcg
     2821 gctatcattc ggcgaacttt atcgggtcgc agcatctatg ccgcggatcg tagtcacatt
     2881 caccattgcc tgatggagaa gtgcaaaagc agtgaaaccg ccctgatggt catttccgca
     2941 tgcgctgcgg ttacagccgt tgcatccttg gggggactgg ttctgggaaa cgacacaatc
     3001 gccctcattg cggccagcgg cgtggtagcc tttttgatcg tcacagggct ctttggccga
     3061 tcggagtgcc gtctcatact caatacactt cgccgctggg gtgacggtct tcgcagatgg
     3121 ctactcggac aagagggtaa gtgcctgtgc aacggcaccc attttcaagg aaagttcgat
     3181 tggcacaagc tttggacaca tctcacagac ccggctggtg aatataacgt gatttatctc
     3241 cgcgttgatc tcaatgaccc acgaatccaa gagggctatt gcggcgaatg gaaaggtcct
     3301 gtatttccca atcccgacga tacgccgatc agtcaccatg aattccctct gtgtactggg
     3361 gatcgcgtcg ttggaaagat caaggcagtc cttcggcgct cagcagaaac attcctaagg
     3421 gatttggagt tcgtcatcag tctcgttgat ccgtttgagc agcagcttga gcaactcaac
     3481 gacgagaggg caagccaaga gcgcgccgat aaggagcatt cggtcggaac acatggatcg
     3541 cacattttca agaacgtcct tcccacaaac tccataaaga tgaccattcc cggcaacggc
     3601 gacggaaatg gaaaaagatc actcatttcc cacggcgcag aaacgactaa aacgccatcg
     3661 atacgggact taagcggagg caaaccaacg gagagtagga aatgagggcg gcaggcaaag
     3721 gatcggctac tcgtcacggc ccgccaggga ctacgaaaac cggggagctt tcgggccact
     3781 cgaccaagac atcccacctg agggattcag ccaccaatgc cgcgcaaaaa cgccggcggg
     3841 ggctgagctt gctccttcgg gccggtctgg tagcgctggg gctccttgtg atcgggattg
     3901 gcgcacgttg ggcagcttct caggctgtgc caaatatcgt gcgagtgccc gagcaagaac
     3961 aactggccgc ctttcccaag caattgggcc cttggaaatg tgaagacgaa gagaagatcg
     4021 atgctaaaat gtttcaagca ttgggaaccg gtgttgccgt gacgcgtcga tacagccagt
     4081 cggcggaacg atttctcgaa atgcatgccg cggtcttcac gcaacctggt ttcagcttgc
     4141 cccacccgcc agagctgtgc tactcgggga caggctggag ggtccgatcg acccgcgatg
     4201 tcccgctaac cctcatcgac ggcacatccg ggagcgtgcg ttttatccgt ttggtaaaag
     4261 aacgcggtga ccagcaagca actgtgctat attgttatca attggcagga ccgttttctg
     4321 ctgatcgcga caaagtgcgg acatttttct ggaaattccg cggacagaaa tcgcgtccac
     4381 cattggtaaa gctaatgctg catctccccg gaagtgggga aaccatagag aacctcggcc
     4441 ttgatttcgc ccaacgcgcg ctcgagaaac tggccgagat ggaaaaaact tggtgaaata
     4501 aacagcgcac ttctttcgct tacaataaaa atgcgaaccg atcccattat cccagactcc
     4561 aggccgacag aggcagaatt aactgcggac cgaccgccgg aaaaagccgg gctcggtgaa
     4621 gacggcgtta acgtcacagc cgacttaacc gcagaggttg ccgacgattg gcaagacagg
     4681 gacgtcgtcg ccgactccga ggcccagccc gcccaggcgg atagtgtccc tccgctcccg
     4741 aaggatctgc cggtcacgat cattcggccg acgggcggat ggcgagcctt ggacctccgt
     4801 gaggtctggc ggtaccgcga attgatgctc accctggcct ggcgtgaaat caccattcgt
     4861 tacaaacaaa cgtttctcgg ggctgcctgg gccatcattc aaccgcttat gacgacaggc
     4921 atttttagcg tcctcttcgc cctcctttta ggcaagggaa atgagccgac ggccggcaat
     4981 gtcccctacg ctgtttccac cttttgtgcc atgttgcctt ggcagctttt tgccacctcc
     5041 ctcaatcagt cgggaaacat cctgattcag taccgcagca tgatcacgaa gatttacttc
     5101 ccgcgattaa ttctccccat ggcggccgtg ctgtcctctt tggtggactt tggcattgcc
     5161 tttgttgcct tgcttgccct catggccttt tatgggattt ttcctggttg ggggcttttg
     5221 acccttccgt tcttcgtagc attggcggtg attgcctcac tctccgtggg actgtggctg
     5281 gcggcactta acgccattta tcgggatttt cgttacgtga tcccgtttat cattcaaatt
     5341 ggcatgtttg ttagcccggt ggtctatagc atggaatcca tccaaggacg actgtccccg
     5401 tgggcgtgga ctttgtattc ccttaacccc atggccggcg tgattgaagg ctttcgctgg
     5461 gcgctgcttg ggacgtctca cggcccaggt ccgcttttgg ccctttcggc cctcgcgacg
     5521 acgatcatcc ttgtgtgcgg aatgttctac ttccgccgta tggaacgcct ttttgcagac
     5581 gttatttgat atgtattagg cgtgggcatt ccccattttg gccgcgcctt gtcgataccg
     5641 taagctaagt taaaacatga tgacctctat gggatgcaag agtaacagcg caacccaagg
     5701 ggccggaggc tgcgctggga taccgatttt gaggtttttg cc
//
LOCUS       DAOPTR010000084         5713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_11499_length_5713_cov_3.740544, whole genome shotgun sequence.
ACCESSION   DAOPTR010000084 DAOPTR010000000
VERSION     DAOPTR010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11499_length_5713_cov_3.740544"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..1158
                     /locus_tag="PKI05_02680"
     CDS             79..1158
                     /locus_tag="PKI05_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008668243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HOA51133.1"
                     /translation="MERTFRIAVLPGDGIGPEVIREAIKVLRVVETHLQDAKFDLTEY
                     PCGAACFLATGEALPAETFEACQRADAILLGAMGMPSVRLPDGREITPQLDLREKLDL
                     FCGVRPIYLYHACDTPLKGYGPGEIDFVIVRENTEGLFSTRTANNSNGEVVSDTLRIT
                     RKGACRLFRAAFELAQTRRRHLTLVDKANVLPSMAFFRRLFLEVAHEYPDVTIDCCYV
                     DAMALYLLKSPQRFDVIVTENMFGDILSDLGAGLVGGMGMAPSGDIGERAAVFQPAHG
                     SAPDIAGRGIANPVAAILSTAMMLEWLKHPSTVRGAKWIREAVRRTLQQPELRTPDLG
                     GRLRTSEMGDLIIKEIEQLWCVSST"
     gene            1140..1769
                     /locus_tag="PKI05_02685"
     CDS             1140..1769
                     /locus_tag="PKI05_02685"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOA51134.1"
                     /translation="MRVLDLTLTISDGMPGVEVKPRHRRPQDAWNSSVLSLYSHCGTH
                     MDSPAHLNSSDSGIDLVPLEQCYGPAWVLRVPNVAPRSLILVEHLGSIAEQLQPGDGL
                     LIATGWSSRWGTPGYREALPRISRELALWCVNRQVRVLGVEPPSVADVNNRQEVEEIH
                     GILLKGGVIIVEGLTNLEAISKPRVIFAAFPLKIQGGDGSPVRAVAFEE"
     gene            1797..3167
                     /locus_tag="PKI05_02690"
     CDS             1797..3167
                     /locus_tag="PKI05_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOA51135.1"
                     /translation="MDNPLQRSRIRIWVITILFFCSLNTYLDRACIASAVDAIRHDLH
                     LSAQAMGLVLGIFALGYALFQVPAGWFADRYGAKKTLAFVVTAWSALTALTGLTWKFW
                     QLLVVRFLFGASEAGAFPGIAQAYYRWLPPGERGLAHGITFSGSRLGAALSLFLMPWL
                     IYHLGWRGMFIVNGFVGLLWVVWWLWTYRDDPRTHPRVTEAELQHIREDSAKTSSNKT
                     TPPLAVLMTSSNMFLAMVQYFASNVTFFVTLTWLPTYLKQQWSNDPSAVYWSAVPLLC
                     ATAANWVAGGMVTRLHSLGYPVASRRITAIVGFGLGTLGLLFAAQTESLFWFVVAFSI
                     TTFGVDMTLSPSWAFCNDLGGKHSGTVSAAMNMVGNIGAAFGAIIFPVLQDSITGRAG
                     TFFFMAASLNVIAIVCWLFMNPMRRLNTSVSTRRLWGRFAVLMAILVILAVGLIGLQI
                     YMTMQK"
     gene            3270..4331
                     /locus_tag="PKI05_02695"
     CDS             3270..4331
                     /locus_tag="PKI05_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA51136.1"
                     /translation="MNAQDRRLKGVCVGAGYFSHYHYDAWQRIPEVEITAFVSRDPNR
                     IRAIQERFGIARVYSDYREALDREKPDFVDIITPPQTHLEICREVAARKIHIICQKPL
                     APTFEEAKTLVREVSQASVRFMVHENFRFQPWYRQIKKILDAGTLGRVFSLYIQTRMG
                     DGWGENAYLDRQPYFRHYPRLLIYETGVHFIDTFRYLLGEIRRVMAWLRRLNPVIAGE
                     DCGLMVCEFYSGAIGVWDANRYNEPNYENPRYTFGQTLLEGEHGSIRIYPDGRLTVQQ
                     LGGPEQPIAYEPSRHGFAGDCCYAAQRHFIDGLLTGQPFETNGWDYLKTLTVQEAVYQ
                     SATTGVPVEVTYGQEGDNV"
     gene            4328..4741
                     /locus_tag="PKI05_02700"
     CDS             4328..4741
                     /locus_tag="PKI05_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOA51137.1"
                     /translation="MSLFHRLDHIAILVADTDRALDVFHRKLGLPLVFSEVLEDQGVR
                     LTHLDMGGTQLQLVQPLRDDIPLAKVLAEKGEGLHHFCFYVEDLNEAVDSLKQNGIGC
                     RDAVPRSAPAGKRAVFLRPEDTYGVLIELTSHPVR"
     gene            4864..>5713
                     /locus_tag="PKI05_02705"
     CDS             4864..>5713
                     /locus_tag="PKI05_02705"
                     /inference="COORDINATES: protein motif:HMM:NF018682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HOA51138.1"
                     /translation="MSSRKSKVVVLDDDPTGTQTVYDIPVITEWSVASLRRELQAAGP
                     GFYILTNSRSLTPAETEQLHWELGKNLLEAAQSYSGRGKTPDLCVISRSDSTLRGHFP
                     LEINILAAALGSPDVVFLVPFFAEGNRLTIDNVHYLGQGQYLVPTGATPFAQDPVFGY
                     KSWNLREWIVEKSQGTIGGERIASISLDDIRRGGPDRVFGIIRTLPRGTFCIVNAADG
                     RDLEVFTLAVLRAEAEGRRFLFRSAASFVAVRLGLQPRPPLPPEELVDAGTRAGGLVI
                     CGSYVPM"
BASE COUNT         1186 a         1459 c         1677 g         1391 t
ORIGIN      
        1 gggcaagaag gtcgggctgg ggcatccccc ggcagaagaa aacgaagatt gggtcatcct
       61 tgttcgaaag tgacaagtat ggaaagaact tttcgcatag ccgtactacc aggggatggt
      121 attggcccgg aggtgattcg ggaagccatt aaggtcctgc gagttgttga gacacacctt
      181 caggacgcga aatttgattt gacggagtac ccgtgcggcg cggcttgctt cctggccacc
      241 ggtgaggccc tgccagcgga aacgttcgaa gcctgccagc gagcggacgc gatcctcctg
      301 ggggccatgg ggatgccttc tgtccggcta cctgatggtc gagagatcac accccagctc
      361 gacttgcggg aaaagctcga tctgttctgc ggggttcggc ccatttattt gtaccacgcc
      421 tgtgacacac ccctgaaggg ttacggcccg ggtgaaatcg attttgtgat tgtccgggag
      481 aacacggaag gtctcttttc gacgcgcaca gcgaacaact ccaatggtga ggtcgtttcg
      541 gacaccctgc gaattactag aaagggggca tgccgattgt ttcgggcggc ctttgaactg
      601 gctcagacgc gacggcgtca cctcactttg gtagacaagg cgaacgtcct cccgtctatg
      661 gcgtttttcc gccgcttgtt cctggaagtg gctcacgagt acccggacgt cacgatcgat
      721 tgttgctacg tcgatgcgat ggcgctatac cttttgaaat cgccgcaacg ttttgacgtg
      781 attgtcacgg agaatatgtt cggtgatatc ctgtctgacc ttggggcggg gcttgtcggc
      841 ggtatgggaa tggcgccatc aggagacatt ggagaacgcg cggcggtctt tcagccggcg
      901 cacggttcgg cgcctgacat tgccggccgt ggcatcgcca acccggtggc tgcgattctc
      961 tccactgcca tgatgctgga atggctgaaa cacccctcaa cggttcgtgg cgcaaagtgg
     1021 atccgcgagg cggtccggcg aacgcttcaa caaccggagc taaggactcc cgatttggga
     1081 ggaagattac gcacgagcga gatgggggat ttgatcataa aagaaatcga gcaactatgg
     1141 tgcgtgtcct cgacttaact ctgaccatca gcgacgggat gccgggagtc gaggtaaagc
     1201 cgcggcatcg acgcccccag gatgcttgga attcgtccgt actcagcttg tattcccatt
     1261 gtggcactca catggatagc ccggcccatc tcaactcttc ggatagcggc atcgacttgg
     1321 ttcctctcga acaatgttac ggtcctgctt gggtgctccg tgttcccaac gttgcaccgc
     1381 gaagccttat cctcgtggag cacctcggga gcattgcgga gcagcttcaa cctggggacg
     1441 gcttactcat cgccacaggc tggagttctc gttggggaac gccagggtat cgggaggcgt
     1501 tgccgcggat tagtcgagag ttggcgctct ggtgcgttaa ccgccaggtc cgagttttgg
     1561 gagttgaacc gccgtctgtg gccgatgtga acaatcggca agaggtcgag gaaattcacg
     1621 gcatcctcct aaaaggcgga gtgatcattg tggaaggatt gaccaacctg gaagcaatca
     1681 gcaaaccgcg cgtcatcttc gcagcgttcc cattgaaaat tcaagggggt gatggttcgc
     1741 ctgtgcgtgc ggtggccttt gaagaatgaa aaagcggtgg agcaaggtgt tggcccatgg
     1801 acaatccgtt gcaacgatca cggattcgga tttgggtgat cacgatcctt ttcttttgta
     1861 gtcttaacac ttacctcgat cgtgcttgca ttgcttccgc ggttgacgca attcggcatg
     1921 accttcactt gtcagctcag gctatggggc tggtcttggg tatctttgcc ttgggctatg
     1981 ccttatttca ggtaccggca ggatggtttg ccgatagata tggggctaag aagactctcg
     2041 ccttcgtcgt cacggcttgg agtgccctca cggccttgac gggtctgact tggaagttct
     2101 ggcagttact tgtggtcaga tttcttttcg gggcaagcga agcgggtgcc tttcccggca
     2161 ttgcccaggc atactaccgt tggctgccgc cgggcgagcg tggattggct catggcatca
     2221 cattctctgg cagccgcttg ggggctgctc tctcgctgtt tctaatgccg tggttgatct
     2281 accatttggg ttggcggggc atgtttattg tgaacggctt tgtcggtttg ctttgggtcg
     2341 tgtggtggct ttggacatat cgcgatgatc cgaggaccca tcccagggtg accgaagcag
     2401 aacttcaaca cataagagaa gatagcgcca aaacaagctc caacaagacg acgcccccgc
     2461 tggccgtgtt gatgacatca agcaatatgt tcctggcaat ggtccagtat tttgcgagta
     2521 acgtcacttt ttttgtgacg ttgacttggc tgccgactta tctcaagcaa caatggtcca
     2581 atgatccttc tgcggtctat tggtcggcgg tccctttgtt gtgcgcaacc gcagccaatt
     2641 gggtggcggg cggcatggtg actcggctgc actccctcgg ttacccggta gcatcgcgtc
     2701 gaattacggc aattgttggc ttcgggcttg gaaccttggg gttgttattt gccgcgcaga
     2761 cagagagctt attctggttc gttgttgcgt tcagcataac gacttttggt gtggacatga
     2821 cgctcagccc aagttgggct ttttgcaacg acttaggggg aaagcactcg ggaacggtct
     2881 ccgccgccat gaacatggtt ggtaatatcg gggcagcctt cggggccatt atttttcccg
     2941 ttcttcagga ttctattacg gggcgagctg gaaccttctt cttcatggca gcgagcctta
     3001 acgtgatcgc tattgtctgc tggttgttca tgaatcccat gcggcgtttg aacacgtcag
     3061 tatccacgcg gcggctttgg ggacgattcg ccgttttgat ggcaatttta gtcatccttg
     3121 cggttggcct gattggcctc caaatttata tgacaatgca aaaataggat cgggcacggg
     3181 gccaataggc caacgcagac agcagacgcc tgatccatca aggattgaaa ttggtgccgc
     3241 gcgggaacgt ccaaaggaga agatcggcca tgaatgccca agaccgacgc ctcaagggtg
     3301 tttgtgtggg agcaggctac tttagccatt accactacga cgcctggcag agaattcccg
     3361 aagtagagat caccgccttt gttagtcgtg atcccaatcg cattcgggca atccaagaac
     3421 gctttggcat tgcccgggtt tattcggatt accgagaggc actcgaccgg gaaaagccgg
     3481 attttgtgga tatcatcacg ccgcctcaaa cccacctgga gatatgtcga gaggtagccg
     3541 cccgcaagat tcatattatc tgccaaaagc cactggcacc aaccttcgaa gaagcaaaaa
     3601 cgctggtgcg cgaggtaagt caagccagcg ttcgcttcat ggttcatgag aacttccgct
     3661 ttcagccgtg gtaccggcag atcaagaaga ttttggatgc cgggacattg gggcgtgttt
     3721 tctcgcttta cattcaaacg cgcatgggcg atggctgggg cgaaaatgcc tatcttgatc
     3781 gacagccgta tttccggcac tatcctcgac tgctcattta cgagacgggt gtgcatttta
     3841 ttgatacatt ccgctatttg ctgggagaga tacgtcgggt catggcctgg cttcgccgct
     3901 tgaaccccgt catcgctgga gaagattgtg gcctgatggt gtgcgagttc tattccggcg
     3961 caatcggcgt ttgggacgcg aatcgttata atgaacccaa ctacgaaaac cctcgctaca
     4021 cattcgggca gacgttgctg gaaggcgaac acggatcgat tcgcatatat ccggacggac
     4081 gtttgactgt ccaacaacta ggaggccctg aacagcccat cgcctacgaa ccatcgcgac
     4141 atggttttgc gggtgactgc tgctatgcgg cgcagcggca ttttatcgat ggactgctca
     4201 caggtcagcc attcgaaaca aacgggtggg actacctgaa gacattgacg gttcaggagg
     4261 ccgtttatca gtcagcgacc actggggtcc cggttgaggt gacctacggt caggaaggag
     4321 acaatgtatg agcttgtttc accgcttaga ccatatcgcc attcttgtcg ccgatacaga
     4381 tcgcgccctg gatgtgtttc atcgcaagct ggggttgccg cttgtcttta gcgaagtcct
     4441 ggaggatcaa ggagtgcgat tgacgcatct cgatatggga gggacgcaac tccagcttgt
     4501 tcagccgctg cgtgatgaca tccccttggc caaagttttg gccgaaaaag gtgagggtct
     4561 tcaccacttt tgcttctatg tggaagatct gaacgaagcg gttgacagct tgaagcaaaa
     4621 tggtatcggt tgtcgcgacg ccgtgccgcg aagtgctccc gccggaaaga gggccgtatt
     4681 tctcaggccg gaagatacgt atggtgtgct aatcgaattg accagtcatc ctgtgcggta
     4741 actggaaacg ctggaatcac catggatagc acggacttct gctcgctttt cccaagggaa
     4801 cgtatattga aggccttgcc acccgtatgg ccagagtcgc tgatggctcg aataagggag
     4861 actttgtctt cccgcaaaag taaagtcgtt gtcttggatg atgacccaac aggaacgcaa
     4921 acggtttacg atataccggt catcacagag tggtcagtcg ccagcttacg tcgcgagctt
     4981 caagcggccg gtcccggttt ctacatcctc acaaattctc ggagtttgac tccggccgag
     5041 acagagcagc ttcattggga acttggtaag aacctgctgg aagccgccca atcctattca
     5101 ggccgtggaa agactcccga tttgtgtgtg attagccgta gcgattccac cttgcggggg
     5161 cactttccgc ttgagattaa tatcttggcc gcggctttgg gctcgcccga tgttgtgttc
     5221 cttgtgcctt ttttcgccga gggaaaccgc ctaacgattg acaacgtcca ttaccttggg
     5281 caaggtcaat accttgttcc tacgggtgcc accccttttg cgcaggatcc cgtgtttggg
     5341 tacaagtctt ggaacctccg ggaatggatc gttgaaaaga gtcaggggac cattggcggg
     5401 gaaaggattg ccagcatctc gctggacgac attcgccgcg gtggtcccga tcgggtcttc
     5461 gggatcattc gcacactacc gcgagggacc ttctgcattg tcaacgctgc agatggtcgg
     5521 gacttggaag tcttcacgct agcggtgctg cgagcagaag cagaggggag acgattctta
     5581 tttcggtcgg ctgcctcatt tgtggctgtg cgactcggtc tgcagcccag gccccccttg
     5641 ccaccggagg aacttgttga tgccggcaca agggcaggag gcttggttat ttgcggctca
     5701 tatgtgccca tga
//
LOCUS       DAOPTR010000085         5673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_11583_length_5673_cov_3.358847, whole genome shotgun sequence.
ACCESSION   DAOPTR010000085 DAOPTR010000000
VERSION     DAOPTR010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11583_length_5673_cov_3.358847"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="PKI05_02710"
     CDS             <1..1069
                     /locus_tag="PKI05_02710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOA51139.1"
                     /translation="LDVALEVVETCHGILGQLGEGAKSLQLDSQLLAPLEGTQACDPG
                     EAKQATDGTACSADAVTGAYASLIGSEEVWRAAEHRACRLARRWGRSVPGCRGLWETV
                     LLVTRRLARLNARFAEALEHAKLDAMAEFAAGAGHEINNPLAIISGHAQVLLRQVTHP
                     DWQRMLAAIVAQAQRAHEMIADARLFARPPKPVFAPVNYRDIVQTVIKEYTPPAESRH
                     VQLRLATAVEAQDRFQGMADRSQILVALGAIVKNALEAVPEKGTITLTLDADTEEVRI
                     SIADDGPGIDPKVREHLFDPFFSGRQAGRGLGMGLSKAWRIVTLHSGTIEVQSAKGSG
                     AIFSIRLPRNPPGGGPSSSGP"
     gene            complement(1248..5639)
                     /locus_tag="PKI05_02715"
     CDS             complement(1248..5639)
                     /locus_tag="PKI05_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOA51140.1"
                     /translation="MAGAAPAPAQPPQSAAEKQQDEQDMAGRLAADGRERRSFKAQSG
                     GKAEDKDKELLAKLTTNRLRDWGSRQDFLTPSVENLQRVGGITRQNFLVLDNKGRLQY
                     FGQNQLAQADVRALAREFQKRGIVLYPEWTPHETAFWDPMVETNDSGKAVVKVVVPDR
                     ATSWKLLAKGVTVDTRVGESEAELQVKKGLFAEVRVPTAFHEGDHVDLPVTIHNDELE
                     QGQIRLVSRVTIGTRTITESRSLEVTRKGLLTVSLPLKAELPEEVRQTIRSAETPEQL
                     AQALQQNLELEVSLHVGDDRTPVDVVRYNVPLQPRGVMEFTSTSGSASSDTTAWLELA
                     PSPDAPWMMEILIGPTIERSLSDIVLGSSQRFLPPCGEGSSLLDRTTSDLMAAVAVLN
                     YFRSIPASADEIVQMVDTRIRIAVSTLVAAQRQDGGWSWSGAKRMRMGASDVDVASHP
                     ESSARAFWALHLARQAGYAVPQATYDNALEYLEKAMTQIRADDMEVRSVLIHALALAQ
                     RADFALVNRLHRERQNLSLAAKLYLALTLAVMQRQPMASEVLKLVDLSKAEEAAQTPL
                     PRSVALSLTEAHALHALVLQAISPESPELRTDVEWLLSHRSGHRWLPDRATGLAALVL
                     GPWFAKTRFIDEKYRLTVFVNDFQVGQWDFDQTSLSQSVLVPPKFFVGGRERIQFRLE
                     GRGRFTYQCLARRFVNPEQARRGHYGSWLRPAPLEVDGREVPRGISSGNEGVFVKPGW
                     SLPHSRVTQLPVGKQAIFEITAAPRVLTAGPVIDQFVLTVPIPAGTSVVPNTIVGPFD
                     RYELRAQEIVFFFARPRSEAVTVNFRVTGTIPGDYVVEPAVIRNALDPTAYAVIPLSD
                     AKDQTSASLKVLPEGVSSTDPYFLSPLELIALGKSAYAKKDWKSAEKYFAELFTNPNW
                     QVMDETVKALAPLLVDIYLELDQPDKVVHYFEILKVKWPDYEIPFDKILRIAACYERL
                     GEYERSFLIFRAILESSFVNDSYTAGFLDEQNEFVRSVQLMNRLLAEYPPEPYAAAAR
                     YALAQRVYAKANEATADMALRKAKITRVHLISAAWEMLDRFLTAFPEDPAADQAAFAA
                     AHALLDCEFYSQAAEAAGRYAQRYAESSLLDSFWYLIGYCHYADGRHEQAIEMCRKVA
                     DWQRKDPATGRTEESKNKWQALYILGQIYHSLGQPLQAINYYQLVSDRFSDAKEAIDY
                     FKRKAVALPEVTFIKPGEPVTLTLRSRNITQCELKAYRIDLLKFTLLKRDFSGIVGIN
                     LAGIRPEYEETIQLGEAQDFRDHEHQVKLPFDKDGAYLIVCRGDDLYASGLVLISPLR
                     MEVQEDAVSGRVRVTIKNVDGNFVNNVHVKVVGSRNAEFVAGRTDLRGVFVADGIQGR
                     VTVIAQKDSNQYAFYRGETDLLPAPARPGAPPAPSERKPQAAGEDRRELLRGLEESNR
                     MLQQQQIENLRQNYQQQRKGVEASKAF"
BASE COUNT         1144 a         1592 c         1709 g         1228 t
ORIGIN      
        1 ccttgacgtt gcccttgagg ttgtggagac ctgtcacggg atacttgggc agcttggcga
       61 gggtgcgaag tcgctacaat tggattcgca actgctcgcc ccgctcgaag gaacgcaagc
      121 ctgtgatcct ggtgaagcca agcaggcgac ggatggcacg gcttgttctg ccgatgctgt
      181 gaccggtgct tatgcgagcc ttataggatc tgaagaggta tggcgggcgg ctgaacaccg
      241 tgcgtgtcgg ctggcacgcc gatgggggcg atccgtcccc ggttgtcgag ggctttggga
      301 gacagtccta cttgtgacgc gacggctagc caggctcaac gcccgttttg cggaggccct
      361 ggagcacgcc aagcttgatg ccatggcgga attcgcggcc ggtgccggac atgaaatcaa
      421 caatccgctg gccatcatct cgggacacgc tcaagttctt ttgcgccagg tgactcatcc
      481 cgactggcaa cggatgctcg ccgcgattgt cgcgcaggcc cagcgtgccc atgaaatgat
      541 tgccgatgcc cgcctctttg cgcggccacc gaagcccgtg tttgcaccag tgaattatcg
      601 ggacatcgtg caaacggtaa taaaggaata cactcccccg gcggaatcgc ggcacgtgca
      661 gctaaggctg gcgacggccg tcgaggcaca ggaccgtttt caagggatgg ccgaccggtc
      721 gcagattctc gtcgccctcg gggcgatcgt gaagaatgcc ctcgaggccg tcccagagaa
      781 ggggacgatc acactgaccc tggatgccga cacagaagag gtgcggatct caatcgcaga
      841 tgatgggccg gggattgacc ctaaagtccg cgaacacctt tttgatccgt ttttttccgg
      901 acgacaagcc ggccgcggtt taggtatggg attgtcgaag gcgtggcgaa tcgtaacgct
      961 ccacagtgga acgatcgagg ttcaatcggc caagggaagt ggagcgatct tttccatccg
     1021 actgccgcgg aatcctccgg gaggtgggcc atcatcttct ggcccgtaag agggatgtgg
     1081 cgagaatttg acgaaactct taaaaccgcc gattgccgcg cctgtccggc agaatggcga
     1141 taatgcgcgg atcctatcgg gtggggctta agggtatctt tgcatcccag ccatttactg
     1201 gagtggatac ctagtatccg gaggcgaggc taaagtgtcg tgattgttca aaatgccttg
     1261 gaggcttcta cgcctttccg ctgttgctgg tagttttgac gcaggttttc gatctgctgc
     1321 tgttgcaaca ttctgttact ttcttccagt ccccgcagca attcgcggcg atcttccccg
     1381 gcggcctgag gtttgcgttc gctcggagcc gggggtgctc cgggacgggc cggcgccggg
     1441 aggaggtccg tttctccccg atagaaggca tactgattgc tgtccttttg ggcgatcacc
     1501 gtaactctgc cttgaattcc gtccgccacg aagacaccgc gcaaatcggt tcggcctgcc
     1561 acgaattctg cattgcggct gccgacaacc ttgacgtgga cgttattgac aaagttgccg
     1621 tcgacattct tgattgtcac gcgaacacgg ccggagaccg catcttcctg aacctccatc
     1681 ctgagcggcg aaatgagaac caatccgctg gcgtaaaggt catcgccccg acacacaatc
     1741 aagtaggcac catccttgtc gaacggcagc ttgacttgat gttcgtggtc gcgaaagtcc
     1801 tgagcttcgc ccaattggat cgtttcctcg tattcggggc ggatcccggc caggttaatt
     1861 cccacgattc ccgaaaagtc gcgtttcaag agggtgaact ttagcaagtc gatgcggtag
     1921 gccttcaatt cgcactgcgt gatgttacgc gatcgcagcg tcagcgtaac gggctcaccg
     1981 ggtttaatga atgtgacttc cggcagcgcg actgccttgc gtttgaagta atcaatcgcc
     2041 tccttggcgt cggagaagcg atcggagacc aactggtaat aattgattgc ctgcaacggt
     2101 tgacctagac tgtggtaaat ctggccaagg atgtagaggg cttgccattt gtttttgctt
     2161 tcctcagtgc ggccagttgc tgggtctttc ctttgccagt cggcgacctt acgacacatc
     2221 tcgatggcct gttcgtgccg accatcggcg taatgacagt aaccgatcag gtaccaaaaa
     2281 ctgtcgagga gcgagctctc ggcataccgt tgggcataac gcccggctgc ctcggcggcc
     2341 tggctgtaga attcacaatc cagcaaggcg tgggcggcgg cgaaggcggc ctggtctgcc
     2401 gcgggatcct cgggaaaggc cgtcaggaac cgatcgagca tctcccaggc ggcggaaata
     2461 agatgcaccc gcgtgatctt ggccttgcgc agagccatat ccgcggtggc ttcgttcgct
     2521 ttggcataga cgcgttgcgc cagggcgtaa cgggctgccg ccgcgtaggg ttccggggga
     2581 tactccgcta aaagccgatt catgagttgc acgctgcgaa cgaattcatt ttgttcgtcg
     2641 agaaagccgg cagtgtagct gtcgttaacg aagctactct ccaagatagc ccgaaagatc
     2701 aggaagctcc gttcgtattc tcccaatcgc tcataacacg cggcgatacg gagaatcttg
     2761 tcaaagggaa tttcataatc cggccatttg accttgagaa tctcgaaata gtgaaccacc
     2821 ttgtcgggct gatccaattc caggtagata tcaaccaaaa gtggggccag agccttgact
     2881 gtttcatcca tgacctgcca gttgggattg gtaaacagct ccgcgaaata cttttccgct
     2941 gatttccaat ccttcttcgc gtaagcggat tttccgaggg caatcagttc caacggcgag
     3001 aggaagtacg ggtccgtgct ggaaacgccc tcagggagga ccttcaacga agccgacgtc
     3061 tggtctttgg cgtcggacaa aggaatgacc gcgtaggccg tggggtccag ggcattgcgg
     3121 atgacagccg gctccacaac ataatctccc ggaatggtcc ccgtcacgcg aaagttgacc
     3181 gtcacagcct cggaccttgg ccgggcaaag aagaatacga tttcttgcgc ccgaagctcg
     3241 taccgatcga acgggccaac aatcgtattg ggtacgaccg atgtgccggc gggaatgggc
     3301 accgttagca cgaattgatc aatgactggg cccgcggtca gtacgcgggg ggcggccgtg
     3361 atctcgaaga tggcctgttt gcccaccggg agttgcgtca cacggctgtg gggtaacgac
     3421 cagcccggct tgacgaaaac cccctcgttg ccgctactta tgccccgggg aacttcgcgg
     3481 ccatcgactt ccaagggggc aggccgcagc catgatccgt agtgtccccg gcgagcctgc
     3541 tcgggattga cgaaccgtcg agcaagacac tgataggtaa atcgcccacg gccttctaag
     3601 cggaactgaa tgcgttcgcg tcctccgaca aagaatttcg gcggaacgag gaccgattgc
     3661 gatagggacg tttgatcaaa atcccattgt cccacttgga agtcgttgac gaacacggtt
     3721 aggcgatact tctcgtcgat aaaacgagtc ttggcaaacc aggggcccag gacgagtgcc
     3781 gccagacccg ttgctcgatc cggcagccag cggtggcctg atcgatggga gagcagccac
     3841 tccacgtcgg ttcgcaattc cggtgattca ggcgaaatgg cctggaggac caacgcgtgg
     3901 agggcatgcg cttcggtcag cgacaaggct acggaccggg gtagcggtgt ttgcgctgct
     3961 tcttcagctt tggagaggtc gacgagcttg aggacttctg aggccatcgg ttgccgttgc
     4021 atgacggcca gtgtcagcgc aaggtagagt ttggcggcga gggagaggtt ttgacgctcc
     4081 cgatggagac gattgacgag ggcgaaatcg gcccgctggg ccaaggccag ggcgtgaatg
     4141 agcacggaac gaacttccat gtcgtctgcc cgaatctgcg tcatagcctt ctcgaggtat
     4201 tcaagcgcat tatcataggt ggcttgggga acggcgtaac ctgcctgtct tgccaggtgg
     4261 agggcccaaa atgcccgcgc cgatgactcc ggatgggacg cgacatcgac atcgctcgcc
     4321 cccatccgca tccgcttcgc cccgctccag gaccaaccgc catcctgacg ctgggcagcg
     4381 acaagcgtac tcacagcgat gcgaattcgc gtatccacca tttgcacgat ctcgtcggct
     4441 gatgcgggga tcgagcggaa gtaattcagc acggcgacgg ctgccatgag gtcagatgtc
     4501 gttcggtcca gcaaggagct gccttccccg cagggtggta ggaaacgctg actacttccc
     4561 aagacaatgt cactaagact tcgctcgatc gtgggaccga tcaaaatctc catcatccac
     4621 ggtgcgtcgg gagagggagc cagttcgagc caggccgtgg tatcgctgct ggccgacccg
     4681 cttgtggagg taaattccat caccccgcgg ggttgaagcg gaacattgta gcggacaaca
     4741 tcgaccggcg ttctgtcgtc gccgacgtgg agactgactt ctaactcgag attttgctga
     4801 agggcctggg ccaattgctc gggggtctcg gcactgcgga tggtttgccg cacctcctcg
     4861 ggaagttccg ccttaagggg taggcttact gtcaggaggc ctttgcgggt tacctcgagc
     4921 gatcggctct ccgtgatcgt tcgagtaccg attgtcaccc gactgacgag cctgatctga
     4981 ccttgctcaa gctcatcatt atggatggtc accggaagat cgacgtggtc gccttcgtgg
     5041 aaggcggtgg ggactctgac ttcggcaaag aggcctttct tgacttgaag ttccgcctcg
     5101 gactcaccca cccgggtatc gacggtaaca cctttggcca acagcttcca tgaggtggca
     5161 cggtcaggaa cgacgacctt gaccacggcc ttgccactgt cgttggtttc caccatcggg
     5221 tcccagaaag ccgtttcgtg gggtgtccac tcgggataaa ggacgatgcc acgtttttgg
     5281 aactctctgg ccagggccct tacgtccgct tgagcgagtt ggttttggcc gaagtattgc
     5341 aatcgcccct tgttatcgag gacgaggaaa ttttgacgcg ttatcccgcc gacgcgctgg
     5401 aggttttcca cgctaggcgt caaaaagtct tgccgggagc cccaatcccg gaggcgattc
     5461 gtagtcaatt tcgccagcaa ttctttatcc ttgtcttcgg ccttgccgcc actctgggct
     5521 ttaaaactcc gccgctcgcg tccgtctgcg gcaagtcggc ctgccatatc ctgctcatcc
     5581 tgttgctttt ccgcggcaga ttgcggaggt tgtgctggtg cgggagcggc tccggccatc
     5641 cctccatagc caaactggcc cgttgccagg gac
//
LOCUS       DAOPTR010000086         5652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_11635_length_5652_cov_3.241201, whole genome shotgun sequence.
ACCESSION   DAOPTR010000086 DAOPTR010000000
VERSION     DAOPTR010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11635_length_5652_cov_3.241201"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="PKI05_02720"
     CDS             complement(<1..795)
                     /locus_tag="PKI05_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51141.1"
                     /translation="MNARDAQHPAVETVVPEAAAYQPLSVAAVVALLLGLVSFTALLT
                     PILWAVAFAGVVVSIFAWVRIHRFDPPLTGKTAAIIGLVLSLFNSAAGPAHWYVRRTM
                     LVQEARQVAELFLDFLKQNQPHKAHQLSLAAAQRSPLDDNLWTKYAPGTSARKDLEDF
                     LDRREVRAIILLAKDPRTLVRFFDVEGVWRENDEDRVALSYAVTYVDPQDKKRTFFVE
                     IALNRYSMRTQPVSDWYISNISSLTPPRTLEAEKAAWDEKQKKQRPL"
     gene            complement(889..1533)
                     /locus_tag="PKI05_02725"
     CDS             complement(889..1533)
                     /locus_tag="PKI05_02725"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOA51142.1"
                     /translation="MWLLMLIQIVIIAACVGFLYREGMWGNFIRFVNVLFAAVLATNF
                     FEPLARILEELISPKLTYFYDFLAFWLVYAVSFLVLHVATAAASRVKVRFNHYLNMGG
                     GIFLALVIGLTMCSVLKFSLHLAPLGTKPFLASVVDEDGDPLGAQWAGIMRGLSSGVF
                     SRSVAAEAEGIYGGSVAKFPGAKNIFEVYLERAKALEAQVNNGGGFTTEGAPPR"
     gene            complement(1626..3410)
                     /locus_tag="PKI05_02730"
     CDS             complement(1626..3410)
                     /locus_tag="PKI05_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOA51143.1"
                     /translation="MDILMINSKSLVLRRLAWVIVLVVIASGWADTVLLAAPWFSPWL
                     TVGQAGSGWSGPGHYINFWKFLAFFLVFLFWVKAGDWVSQDSQRLDLRPNTWNTVIFF
                     VFVAAFVLGLLIPVFPVSLLLVLLAAIVPFAIYVVQRNGKVGAEERVFTVLHFKQIIG
                     RLSGKKVVVPLSPDDLPPPIRFEPIAKTQQEKNLRLIPARQHPGYQAAREFIAQTLDR
                     RGEAVDLMVAPTATTMRYMIDGTWVNMPPIPPETAMGAVEVLKILCGLNPQDRANRQQ
                     GKLDLKYHDVPLSLTFASQGVTGGERVVIQFVEKKPRFRKLEDLGMRPKLQEMIKSFL
                     ETPQGIIVFSAPPGHGLTTTVTCALLTGDRYSREWISIEDEQKTYEVIENVQPHIIKA
                     EDRQNLEQVLDKIFHLEPNVVVYRDVPDGKVLERLAQETENNRLIITTVRAKDCCDAL
                     QKLMALPPPSKTFPEKVIGVVNQRVMRRLCDKCRQPYAPPAELLQRLGIPPGRIQAFF
                     RPRVPTPEDKEVCPQCQGLGYFGRAAVFEVLAVNDNLRKLLARRPDYATMRKAARAAG
                     MATLQEEGVLLMAQGITSYEELMRVLQG"
     gene            complement(3410..4558)
                     /locus_tag="PKI05_02735"
     CDS             complement(3410..4558)
                     /locus_tag="PKI05_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOA51144.1"
                     /translation="MATTQETVRPVFGKRPRNLEIDKLFRALVKFEGSDLHLKVGRPP
                     FVRVQGSLRPLNLPPIDEDEMVRLILPMLDERLRRILEEEGGCDFAHVVDVDGVSWRF
                     RVNVLQQMGYLGLVARRINNYIPDFEKLNLPPILENLCKFDQGMILLAGVTGSGKSTT
                     IASMLNWINRHYRKHILTLEDPIEFTFTEDKCLINQREVGIDVKNFIIGMKHAVREDP
                     DVMLVGEMRDRETFETAIQAAETGHLVFGTIHASSAPSTISRILDLFPQSMHRAIRSA
                     IAFNMKAIIAQKLLPSINPEVKRVPAVEIMIFNPMVKKLILEEQDQKLADIIRMSKEE
                     GMQDFTMSLKSLVERRLIDRATAFEVAPNPEALQMALKGIEVKAPGII"
     gene            complement(4711..5478)
                     /gene="hisF"
                     /locus_tag="PKI05_02740"
     CDS             complement(4711..5478)
                     /gene="hisF"
                     /locus_tag="PKI05_02740"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOA51145.1"
                     /translation="MLAKRIIPCLDVDRGRVVKGTRFVNLRDAGDPVEVAARYEEEGA
                     DELVFLDITASHEGRDIILDVVRRTAEVVFMPLTVGGGIRTLDDIRRLLNAGADKVSI
                     NSAACRNPDFVYQASRRFGSQCIVVNIDPKRVIKDGREFWEVHINGGRVGTGLEAVAW
                     AKEVERLGAGEIVLTSMDADGTQKGYDLEITKAVADAVTIPVVASGGAGCPEHMADAI
                     LKAGADAVLAAGIFHFGQFTITETKQYLKRCGIPVRL"
BASE COUNT         1360 a         1696 c         1364 g         1232 t
ORIGIN      
        1 caaaggacgt tgctttttct gcttttcgtc ccaggccgct ttctccgcct caagagttcg
       61 cggcggcgtt aaactactaa tgttgctaat ataccagtca gagactggct gcgtcctcat
      121 ggaatagcga ttgagagcga tctccacaaa gaacgttcgc ttcttatctt ggggatcgac
      181 gtaggtgaca gcatagctga gcgctacccg atcctcgtca ttctcccgcc aaaccccctc
      241 cacatcgaaa aagcgaacca atgtccgggg atccttcgcc aagagaataa tcgcgcgaac
      301 ttctctccga tcgagaaaat cctcgaggtc ttttcgggca ctggtccccg gggcatactt
      361 cgtccagagg ttatcatcca gtggggagcg ctgggccgcc gctagactca actgatgggc
      421 cttatggggc tgattctgtt tcaggaaatc gaggaacaat tccgccacct gccgggcttc
      481 ctggaccagc atggttcgcc gcacgtacca atgggccggc ccggccgcag agttgaacag
      541 actgagaacc aatcctataa tggcggcggt tttgcccgtc aatggtggat caaatcggtg
      601 aatacgaacc caggcaaaga tgctgaccac tacccctgca aaagccaccg cccaaaggat
      661 cggggttaac aaggccgtaa aagacaccaa ccccagcagc agtgccacga cagccgcgac
      721 acttaggggc tgatacgcag ccgcttcggg aactaccgtt tccaccgcag gatgttgggc
      781 atcgcgagcg ttcaatttgc catctccctc atctcttacc ttgtcatggc cccacactcg
      841 gtacccattt tcaagttaac cagccacgtc tacgatcagc acagatcatt accgcggagg
      901 agccccttcg gtggtaaagc caccaccatt gttcacctgg gcctccaacg ccttcgcccg
      961 ttccaagtaa acttcaaaaa tattcttggc acccgggaac ttggcaaccg agccgccgta
     1021 aatgccttct gcttcagctg ccacactccg cgaaaagacg cccgaactta gcccccgcat
     1081 gatccccgcc cactgagccc cgagaggatc cccatcctca tcgacgacgg aggccaaaaa
     1141 aggctttgtt cccaaagggg ccaggtgcag cgagaacttc aacacggagc acatggttag
     1201 gccaataacc aaagcgagga aaattccgcc acccatgtta aggtaatggt taaaacggac
     1261 ctttacacgg gacgccgcgg ccgtggccac atgcaggaca agaaacgata ctgcatacac
     1321 cagccaaaaa gccaggaaat cataaaagta ggtcaacttg ggacttatta gttcttccaa
     1381 gatcctggcc aagggttcaa agaagttcgt tgccaaaaca gcggcaaaca gaacattcac
     1441 gaaccgaatg aagttacccc acatcccctc tcgatataga aatcccacgc aagccgcaat
     1501 gataacaatc tgaattagca tcagaagcca catcgtcggt ctccacaact gttagccctc
     1561 ggtcatccgc gttcacgagg gacgtgatgg cacgattcgc tttcctaatc ttcaaacttt
     1621 tctcatcaac cctgaagaac ccgcatcaat tcttcgtacg aggtgattcc ttgagccatg
     1681 agcaagacgc cctcctcttg caaggtcgcc attcccgcgg cccgggcggc ctttctcatg
     1741 gttgcatagt ccggtcgcct tgccagcagc ttgcgcaggt tatcgttgac cgcaaggacc
     1801 tcgaagacgg cggcacgccc aaagtatccc aacccctgac actgcggaca cacctcctta
     1861 tcctccgggg tgggaacacg cggccggaaa aaggcctgaa tccgccctgg cgggatcccc
     1921 aaccgctgca ataactctgc cggtggagca tacggctgac gacacttgtc gcacagtcgt
     1981 cgcatcaccc gctggttgac aacgccgatg accttttccg gaaaggtctt cgacggcggc
     2041 ggcaaagcca tcaatttctg cagggcatca caacagtcct tggcgcggac cgtggtgata
     2101 atcagccgat tgttctccgt ttcctgggcc agccgctcca agacctttcc atccggcacg
     2161 tcgcggtaaa caaccacatt aggttcaagg tggaaaatct tgtccaaaac ctgttctaga
     2221 ttctggcggt cttcagcctt aatgatgtgt ggctgaacgt tttcaattac ttcgtacgtc
     2281 ttctgttcgt cctcgatgct gatccactcg cgcgaatacc gatccccagt cagcagagca
     2341 cacgtgaccg tggtcgtcag gccatgacct ggtggggcag aaaagacaat gatcccctga
     2401 ggggtctcga gaaaactttt aatcatctct tggagtttcg gtcgcatccc caagtcttct
     2461 agcttgcgga atcgcggctt tttctccacg aattggataa cgacacgctc gccaccggtg
     2521 acgccctgcg acgcgaaggt caaacttaac gggacgtcgt gatacttcaa atcgagtttc
     2581 ccctgctgac ggttggcacg gtcttgtggg ttaagtccgc acaaaatctt caggacctcc
     2641 acagccccca tcgctgtctc cggcggtatc ggtggcatat tcacccaagt accgtcgatc
     2701 atatagcgca tagtggtggc cgtaggggca accatcagat cgacggcctc tccgcgacga
     2761 tccagcgtct gggcaatgaa ctcgcgggcg gcctgatatc ccggatgctg cctggccggg
     2821 atcagccgta gattcttttc ctgctgcgtc ttggcaatcg gttcaaatcg aatgggcggg
     2881 ggaagatcat ccggcgacag cggaacaacg accttcttgc ctgatagtcg gccgattatc
     2941 tgcttaaaat gcaatacggt aaaaacacgc tcttcagccc ccactttacc gttgcgttga
     3001 accacataga ttgcgaacgg tacgatggcc gcaaggagga cgagcaacaa acttacagga
     3061 aacaccggga tgagcagccc caatacgaag gccgccacaa acacgaaaaa aatcacggtg
     3121 ttccatgtgt tcggtcttaa gtccagacgt tgactgtctt gactaaccca gtcaccggcc
     3181 ttaacccaaa acaaaaacac gaggaaaaat gcaagaaatt tccagaagtt gatgtaatgc
     3241 cccggtccgg accaaccact gcccgcttga cctaccgtta gccagggaga aaaccaggga
     3301 gcggccagca gaacggtatc ggcccaaccc gaagcgatca caaccaagac gataacccaa
     3361 gccaaccttc gcaaaaccaa gctcttgctg ttgatcatga ggatgtccat caaataatcc
     3421 caggtgcctt gacttcgatc cctttcaaag ccatctgcag ggcctccgga ttgggggcaa
     3481 cctcgaaggc cgtcgcccga tcaatcagtc gccgctccac cagactcttt aagctcatcg
     3541 tgaaatcttg cataccctct tctttactca tccggataat gtcggccagc ttttgatctt
     3601 gctcttccag gatgagcttc ttgaccatgg ggttaaaaat cattatctca accgccggaa
     3661 cccgtttgac ctctggatta atcgacggca gcagtttctg ggcgatgatg gccttcatgt
     3721 tgaaggcgat cgcgctacga attgccctgt gcatcgactg tgggaacaag tcgagaattc
     3781 ggctaatcgt actcggtgcg ctggaggcgt gaatggtccc aaaaacgagg tggccggttt
     3841 cagcggcctg gatcgccgtt tcaaacgttt cgcggtcacg catttcaccg accagcatca
     3901 cgtcagggtc ttcacgcacc gcgtgtttca tgccgatgat gaagttcttc acatcaatcc
     3961 caacttcccg ctgattgatc aggcatttat cctccgtgaa cgtgaactca atcgggtcct
     4021 caagtgtgag gatatgcttc cgataatgcc gattgatcca gttgagcatg gaggcaatcg
     4081 ttgtactctt ccctgatccc gtcacaccgg ctaggaggat cataccctga tcgaatttgc
     4141 aaaggttttc cagaataggc ggcaggttca gtttctcaaa atcggggatg taattgttga
     4201 tccgtcgggc caccagtccc aagtaaccca tttgctggag aacattaaca cgaaagcgcc
     4261 atgagacgcc atcgacatct accacatggg cgaagtcaca accgccttct tcttcgagga
     4321 ttctgcgtaa ccgctcgtca agcatgggca gaatgagccg caccatctcg tcttcatcaa
     4381 tcggcggtaa gttcaggggg cgaagcgacc cttgaacccg cacgaacggc ggccgtccca
     4441 ccttcaggtg taagtcactc ccttcaaact tcaccaatgc ccgaaacagc ttgtcgattt
     4501 ccaaattacg cgggcgtttc ccaaaaacgg gacgaacggt ttcctgtgtg gtcgccataa
     4561 tgtgacctcg attccccgaa tttccgaaat acccaccttg cgcgccatga cgtatcctgc
     4621 gcgctaagac ttctcaaccc cagcgtctct ctagccttct tcaagccatt atgccctgga
     4681 ttgttcgtca ggaaaacacg ggcacaatcg ctacagccgc acagggattc cgcagcgctt
     4741 caagtactgc ttggtttcgg taatcgtaaa ttgaccgaaa tgaaagatcc cggcggcgag
     4801 aacggcatcc gcgcccgctt tgagaatggc atccgccatg tgctcgggac agccggctcc
     4861 cccactggct accaccggga tggtgacggc atcggccacg gcttttgtga tctcaagatc
     4921 gtagcccttc tgcgtaccgt cggcatccat cgaagtcagc acaatctcgc ccgccccaag
     4981 tctttcaact tccttggccc aggccacggc ctccaaaccg gtgcccactc gaccaccgtt
     5041 gatatgaacc tcccaaaact cccggccatc cttaatcacc cgttttgggt cgatgtttac
     5101 cacgatgcac tgactgccga atctgcgaga agcctgataa acaaagtcag gattacgaca
     5161 tgcagccgaa ttaatggaga ccttatcggc ccccgcattg aggagacgcc gaatatcgtc
     5221 gagagtccga attccacccc ctaccgtaag gggcataaaa acaacctcgg cggtgcgtcg
     5281 aaccacgtcg agaataatgt cgcggccttc gtggctggcc gtaatgtcga gaaaaaccag
     5341 ttcgtcggcg ccttcctctt cgtaccgtgc ggctacttcc acaggatcgc ccgcatcccg
     5401 cagattaaca aatcgcgttc ctttaaccac ccgaccacga tctacatcaa ggcaggggat
     5461 gattcgtttg gctaacataa tctactcttg gcagttgcaa acccgccggt taaccggggc
     5521 ttaagcccat cgggactagt atgttgcttg ctacctgtga aaccgcccgg ttctctgtcc
     5581 atttttgctc atgtacctaa aaatcccatt ggtgaccgca agcaggtatg acttcttctc
     5641 actgtaaatg gc
//
LOCUS       DAOPTR010000087         5620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_11731_length_5620_cov_3.657502, whole genome shotgun sequence.
ACCESSION   DAOPTR010000087 DAOPTR010000000
VERSION     DAOPTR010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11731_length_5620_cov_3.657502"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PKI05_02745"
     CDS             complement(<1..584)
                     /locus_tag="PKI05_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51146.1"
                     /translation="MTLHHGIDEQNLRREPLAELLKREAEAERPAFSEDLYGRILNQV
                     GIPGAERAHLFSSGKSRLSFKQIAWVVLATYASVVTVAILMQVVQRSGWFHRQHIEVS
                     GIAGENLNEPRQEKGSGSDVNLVHGKPPTEAIEDLVSAPDRTIQQVQIALSEIDRQRW
                     ANLDNDLRLAGELLHQQLPWGVLSRAESSQVETP"
     gene            complement(708..1316)
                     /locus_tag="PKI05_02750"
     CDS             complement(708..1316)
                     /locus_tag="PKI05_02750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOA51147.1"
                     /translation="MSHLASLTSLTDEQLAEQAQMGCLASFAELVRRYQVPVLHFLQR
                     KCRSSTDAEDLTQETFLRIFRKLSHYRVGAPFRPWVFTIAYRVALNGMRGHRYSTVGE
                     NEKSSRITQVDSLLEDAEENHRLWDAVQAELSPIQFTAVWLFYVEELSIRQIAAVIGK
                     SESAVKTILCRARKVLVHRLRAYAKFNTLPVSEDEAVSPERH"
     gene            complement(1474..2424)
                     /locus_tag="PKI05_02755"
     CDS             complement(1474..2424)
                     /locus_tag="PKI05_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51148.1"
                     /translation="MRILKLLVIVALIGVAGIAWTPLAWASELDWGQVPDNWRWIVHF
                     DIDQLRDSKVAATLFSEFWHAHPERHKIEAIAALAGLNLKKDLHGVTFLGTRFDPKFG
                     AMIIHCRMNPDLLVGLIASEPTYRRRESKGLILHQWTDRNDNSEITGCFLDQDHLVLG
                     KDATAVEVIIERYQQKSSQERPDFFNVRKGTYLQIHANELQELSVPFMSPLLRRSRRL
                     SAMVGEQSGQFFLQCSFEVEGAEIALDLKDALTGLVAIGKLHYADRPEYVAGLTLIRV
                     FASENKTTMVWTMPAEPALRVLKEIASQVATEGKKSGGLP"
     gene            2673..4256
                     /locus_tag="PKI05_02760"
     CDS             2673..4256
                     /locus_tag="PKI05_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51149.1"
                     /translation="MPNRREFLGMGCGLAMSGLLGSAGTLLCPKHGSASTGSEGEETI
                     RTDFLDGSSKSVAWLEEMVGPSLGRLLTKTSREIRSSPFSVGLETLDRSMYDPERVYD
                     PLGKLGVKWARLQTGWARTEQIVGQYDFGWLDAVVDAVVGQGVAPWFNVGYGNKLYTP
                     EAPDASAVGWIPIYNADAQQAWLRYVRALAQRFGERVKHWEIWNEPNIKNFWKPGEPS
                     PELYVEFVAQTAKVIREVIPQVTIIGGALAGMPTDYLKKLLSCGLAQHVDKISFHPYR
                     AIPEDGYRDTLETWKQLLQSAGEKRLQLWQGENGCPSRPGSAGALAQFPWNEITQCKW
                     LLRRVLYDRVLGLELTSYFHMVDLMRYNWGQGPTDKANWKGLLRGEDYSPKPAYFGYQ
                     RICTLFDDDARRDISLDTSVEVVEENRADPASSRSSKEKRFIPPAAFVRNNSPMWCFW
                     YASDLIKPGEITYLTLTIREKALSSGELFYLDLLSGWIFRLSKLERQNDQCHVRSIPV
                     GDYPVVIGAPGALQGLVSS"
     gene            complement(4510..4773)
                     /locus_tag="PKI05_02765"
     CDS             complement(4510..4773)
                     /locus_tag="PKI05_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51150.1"
                     /translation="MAKQVVKKAAKKAAKKAAKKPAKKAAKKAAKKPAKKAAKKPAKK
                     AVKKAAKKVAKKPAKKPAKKPAAKKAPAAPPAPAETTPPPAGG"
     gene            complement(5093..>5620)
                     /locus_tag="PKI05_02770"
     CDS             complement(5093..>5620)
                     /locus_tag="PKI05_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA51151.1"
                     /translation="VVAYTETFVKERVHAETGKVEKVNIDDACMFLAVFENGSLGTFE
                     STRYARGRKNYNTFELNGELGSVFFDLEDPHILQFFRYADPETGKKIEDHLTGWQRIH
                     VTHSEHPYMKHWWVPGCTIGYEHTFTNSFADFLASLEGGPKFQPDMRSALRTQRVCDA
                     VLQSAREGRWIEIPA"
BASE COUNT         1262 a         1509 c         1481 g         1368 t
ORIGIN      
        1 taaggagtct ccacctgaga actttccgca cgggagagaa caccccaagg aagttgttgg
       61 tgaagaagtt cccccgccag ccgaaggtca ttgtccaaat tggcccaacg ctgacggtcg
      121 atttcgctga gggcgatttg gacctgctgg atggtgcggt caggggccga gaccaaatcc
      181 tcgatggcct cagtaggcgg ttttccgtgc acgaggttca catcgctccc gctgcccttt
      241 tcttgccggg gctcattcag gttctccccg gctattcctg aaacctctat atgttggcgg
      301 tgaaaccatc cagacctttg aacaacctgc atcaggatgg ctacggtcac cacgctcgca
      361 tacgtcgcca agactaccca ggcaatctgc ttgaaggaaa gccgcgattt accggaggaa
      421 aacaaatgag cgcgctcagc accaggtatg ccgacctggt tcagaatccg cccgtaaagg
      481 tcctccgaaa acgcgggtcg ttcagcctct gcttctcgtt tgagaagctc ggctaacggc
      541 tcccgtctca gattctgttc gtcaattcca tgatggagcg tcatgattga ctcaccctac
      601 tgatgatccc aggagttacc ttttgtttgc cctataaact gggtcaattc gtgacttcga
      661 tgcactacga cagcgggtaa caaattgttt tctaggtaat gtgcccgtta gtgcctttcc
      721 ggtgaaacgg cctcatcttc ggagacgggc aacgtgttaa actttgcata agcgcgtaat
      781 cgatgtacga gtacctttct tgcgcgacag aggatcgttt ttacggcact ttctgatttt
      841 ccgatcactg ccgcgatttg tcggatactc agttcttcca cataaaatag ccacacggcg
      901 gtaaactgga tcgggctcaa ttcggcctga accgcatccc acaagcggtg attttcttcc
      961 gcatcctcca ataaagaatc aacctgagtg atgcggctgc tcttttcgtt ttcccccacc
     1021 gtggaatacc gatggcctcg catcccgttc aaagccactc ggtaagcgat ggtaaaaacc
     1081 cacggccgaa agggtgcacc aacgcgataa tgcgacagtt ttcgaaagat gcgaaggaac
     1141 gtctcctgcg ttaagtcctc ggcatctgtt gatgaacgac acttccgttg caagaaatgc
     1201 agcaccggca cttggtaccg gcgtaccaac tcggcaaaac tggctaagca gcccatttgg
     1261 gcttgctcgg caagttgctc atcggtcaaa gatgtcaatg aggccaagtg tgacatcttg
     1321 ctcgtcgagt tattggcggc acagacacct aggtgcaatg acttttgtca cgcggaccac
     1381 ggtattcgat ggttcgcaac cgccatttgg gcatttcttc catactgtcc cagttttcta
     1441 aacattgcct tgaaaacgta cgcaattacg tgattaaggc agtcccccag attttttacc
     1501 ttccgtagct acttgagatg caatctcctt aaggacccgc aaggcaggtt ctgctggcat
     1561 cgtccagacc atggtggtct tgttttcgga ggcaaaaaca cgtatgagcg tcaaacccgc
     1621 cacatattca ggccgatcgg cataatgtag tttcccaatc gcaacgagtc cggtgagcgc
     1681 atcttttaga tctaaagcaa tctctgcccc ctcaacttca aaggaacact gaaggaagaa
     1741 ctgtcctgac tgttccccaa ccatggccga taatcggcga ctgcgacgga gcagcggcga
     1801 cataaagggg actgatagtt cttgcaactc attggcatga atctgcaagt acgtaccctt
     1861 gcgaacgttg aaaaaatcgg gcctttcctg agacgatttt tgttggtacc gctcgatgat
     1921 cacctcaacc gccgtcgcat cttttccgag cacaagatgg tcttggtcga gaaagcagcc
     1981 tgtgatctcg ctgttgtcgt tacggtctgt ccactggtga aggataaggc cttttgattc
     2041 tcgtcttcga tacgtcggct cgctcgcaat cagacccacc aataaatcag gattcatccg
     2101 acagtgaatg atcatggcac cgaatttggg atcgaagcgt gtccccaaaa aggtcacgcc
     2161 gtgaaggtct ttcttcaaat tgaggccggc cagcgcggcg atggcttcaa tcttgtgtcg
     2221 ctccggatgc gcgtgccaaa actcggaaaa aagagtggct gcaaccttcg aatcacggag
     2281 ctggtcaatg tcaaagtgaa caatccatcg ccagttgtcc gggacctgtc cccagtcaag
     2341 ttcagacgcc caggccagag gcgtccaggc gatcccggcc actccaatta gggcgacaat
     2401 gaccaaaagt ttcaagatgc gcataatgtc aacttttttg tgtgactttt agcagtccgc
     2461 ctgcaactgt ttgctcctat ccaatataca cgccgtgcac ctcgcaggtg acaaacgatt
     2521 tgacttaggt ttacctctga caagggcaac caggcggcaa ggtgcgcggg acgtcccgac
     2581 ttgttgcata tgaatcgcca ctgtttgaca atggcggtgt cgtgaatcgg tttgtttttt
     2641 ggtcgtgtct tgatcaacgt aaaggagcca gtatgccaaa ccgaagggaa ttcctgggca
     2701 tgggttgtgg tcttgcgatg agtgggcttt tgggctcggc ggggacactg ctttgtccga
     2761 agcatggatc ggcttccaca ggatcggaag gcgaagaaac cattcggaca gatttcctcg
     2821 acggctcatc aaaatcggtg gcctggctgg aggaaatggt aggaccctca cttgggcgac
     2881 ttttgacaaa aacgtctcga gaaatccgca gttcgccctt ttctgtaggg ctggaaacgc
     2941 tcgaccgatc catgtacgat cccgaacggg tttatgatcc cttgggaaaa ttaggagtca
     3001 aatgggcaag gctgcaaacc ggctgggcac gaaccgaaca aattgttgga caatatgatt
     3061 ttggctggct cgatgccgtc gttgatgccg tggttgggca aggcgtagcg ccatggttta
     3121 acgtgggcta cggcaacaaa ctatataccc ctgaggcgcc cgacgcctcg gccgtgggtt
     3181 ggattccaat ctacaacgcg gacgcccagc aggcctggtt gcggtacgtt cgcgcgctgg
     3241 cccagcgatt cggcgaacgg gtcaaacact gggaaatttg gaatgagccg aatatcaaga
     3301 acttttggaa accgggtgag ccctcccccg agctttatgt cgagtttgtt gcccaaacgg
     3361 ccaaggtcat tcgagaggtc atcccgcagg tgaccatcat cgggggtgcc ttggccggga
     3421 tgccgacgga ttatttgaaa aagctattgt cctgcggttt ggcccaacat gtggataaga
     3481 tttccttcca cccttatcgg gctattcctg aggatggcta ccgggacacc ctggagacat
     3541 ggaagcaact tttgcagagc gctggcgaga agcgtctcca attgtggcaa ggggaaaacg
     3601 gttgcccttc tcgaccggga agcgcggggg ctttggcgca gtttccttgg aatgagatca
     3661 cacagtgcaa atggctatta cggcgcgtcc tctatgaccg ggttttggga ctggaattga
     3721 catcatattt tcacatggtc gatttgatgc ggtacaactg gggccaagga ccgacggata
     3781 aagcaaattg gaaaggactg ctgcgcggtg aggattattc gcccaagccc gcctatttcg
     3841 gctatcagcg aatctgcacg cttttcgatg acgacgcacg acgtgacata agtctagata
     3901 ccagtgttga ggtcgtcgag gagaataggg ccgaccccgc aagttcgcgg tcgtccaagg
     3961 aaaaacgatt cattccaccg gccgcttttg ttcgtaacaa ttctccgatg tggtgtttct
     4021 ggtacgccag tgatttgatc aaacccggcg agataaccta cctgacccta acgatccggg
     4081 aaaaagcgct ttcgagcggg gaattgttct acctggacct gctaagcggt tggatcttcc
     4141 gcttgtcaaa acttgaacgc cagaatgacc agtgccatgt tcgaagtatt cccgtcgggg
     4201 attatccggt ggtgatcgga gcgccggggg ctttacaagg tctcgtttcc tcgtgaccgt
     4261 tgcctcagaa atggtgaggt ggaacgccga accaaagacg ggcacggctc caagaccagg
     4321 cttgacgagc gagcgtcctc gacgaggtga tgtggtcgcg ggaaaccgaa cgggatcata
     4381 gcacactttg ttcccttcca tttgatggaa gcagccaaaa aagataggga cacaacctga
     4441 tgaggctgtg tccctttctc gtcgttgtac gaaatcaggc tgctgccggt tgcctcccca
     4501 gcgtcgaaat cagccgccag cgggaggcgg cgtggtttcc gctggggcgg gaggggctgc
     4561 gggagccttc ttcgccgccg gcttcttggc cggttttttg gccggcttct tcgcgacctt
     4621 cttagcggct ttcttcaccg ccttctttgc cggcttcttg gctgctttct ttgccggctt
     4681 cttcgcagcc ttcttggcgg ctttctttgc cggcttcttc gcagccttct tggcggcttt
     4741 ctttgccgcc ttcttcacaa cttgcttggc caccttcaaa acctccttac tgaaggacac
     4801 cctgggagcc gttgtggaag acatatgcca cgtaactgat ccacaacgcg caccctatca
     4861 caacaccaac taccacgtgc cggtttccgc acctaagcgg attgcaccta agtcaacgtc
     4921 cgtctttgct tgcttagctg ctgccgcgtt tttcacagca gcagcctaaa aatatgttaa
     4981 cgttttcttt caaaagtgca agttgaatct caagctcaaa tgggcaaatt gatcgggtga
     5041 tcagatgccc attgtcttca tccgtccgtg gcagtgctat acagcgttcg ctttaagccg
     5101 ggatttctat ccagcggccc tcgcgggcac tctggagcac tgcgtcacaa acgcgctgtg
     5161 tccgcagtgc cgaccgcata tccggctgga actttggtcc gccttccagc gacgcgagaa
     5221 aatcggcaaa cgaattcgta aatgtgtgtt cgtagccaat cgtacatccg ggaacccacc
     5281 agtgcttcat atagggatgc tcactgtggg tcacatggat tctttgccag cctgtcaaat
     5341 gatcttcgat cttctttccg gtttctgggt cggcatagcg gaagaactgc aaaatgtgcg
     5401 gatcttccag gtcaaagaac acagaaccaa gttctccgtt caactcaaag gtattgtagt
     5461 tcttgcgacc ccgagcatac cgcgtgctct cgaaagtgcc gagtgaccca ttttcgaaga
     5521 cagccaggaa catgcaggca tcgtcgatgt tcaccttttc gactttgccc gtttcggcat
     5581 gcacccgttc cttcacgaag gtctcggtat aggcaacaac
//
LOCUS       DAOPTR010000088         5618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_11737_length_5618_cov_4.192163, whole genome shotgun sequence.
ACCESSION   DAOPTR010000088 DAOPTR010000000
VERSION     DAOPTR010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11737_length_5618_cov_4.192163"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PKI05_02775"
     CDS             complement(<1..704)
                     /locus_tag="PKI05_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HOA51152.1"
                     /translation="MTSKTAQRLLIVSNRLPLRVVKEREGTWRLERGSGGLVTALAPV
                     LRNRGGAWIGWPGVPAEKSSGNLSELMDKAGKDLGYRIVPVALSRREQRLFYEGFSNE
                     VIWPLFHEFPSLAQFKPSYWDAYRSVNRKYAEIIASHSQSDHFIWIHDYQLMLVAQSL
                     RDLGVQSHLAFFLHIPFPPLDVFLKLPWRLEVLRALLQYDLLGVQTARDRRNVIECFR
                     ALMPDVTWRGRGRVVVG"
     gene            complement(694..1497)
                     /gene="otsB"
                     /locus_tag="PKI05_02780"
     CDS             complement(694..1497)
                     /gene="otsB"
                     /locus_tag="PKI05_02780"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HOA51153.1"
                     /translation="MEVLKYPNRLASTFKRLRAGTRGVLLCDYDGTLAPFREDPSEAR
                     PYTWVPGILGDIAAWGTRVLIVSGRAADDLLKLLHLEAIEIWGSHGRERIQPNGTRWV
                     WKPNAETEHLLDRIENELRRLAPGKRLERKVGCLAIHWRGLSQTDAEWLQKTVDELRA
                     RFDKHGVLMVKAFHMGVELQAPGPNKGSVVSTILDEEKGADIAVCYMGDDQTDEDAFQ
                     ELGERGLNVLVSPTPRLTFADVRLETGDEVRLFLEQWRDAVRGGCDHDE"
     gene            complement(1485..2738)
                     /locus_tag="PKI05_02785"
     CDS             complement(1485..2738)
                     /locus_tag="PKI05_02785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA51154.1"
                     /translation="MEGLLERYADVAGADAVTQLRQLAEPLQGIRVIHVNSTRVGGGV
                     AEILSQLVPLLQELGLDVEWCTIEGNPDFFACTKKFHNCLQGAGDRPSEQDLRIYEKI
                     NAENAERLKAVLTEADVVFIHDPQPAALIRYFPERHGIWIWRCHIDVSHPDRSVWRYL
                     RQFLKEYDASIFSLADFARALPHPQYLIAPAIDPFSGKNCELSQNFIEGVVKDFSLDL
                     NRPLLLQVSRFDRFKDPIGVIKAFRLVRSFFPSAQLVLAGGGATDDPEGGAVLAEVRA
                     AAAGDPAIHILELPSDAHRIINALQRAAWIVLQKSTREGFGLTVTEALWKAKPVIGGN
                     TGGIRLQVINHHTGFLVSSPEGAAMRIRYLLERPEQARRMGEKGQKYVRENFLMTRLA
                     RDHLTLMVSLLRQEIERIDTTQWKS"
     gene            complement(2738..3388)
                     /locus_tag="PKI05_02790"
     CDS             complement(2738..3388)
                     /locus_tag="PKI05_02790"
                     /inference="COORDINATES: protein motif:HMM:NF039842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HOA51155.1"
                     /translation="MTFVIKDCALIPISTGMRASNLSEFKFALNAAPPECIYHHFWGA
                     LLQSRFDDPEYKNDFAVWVYRDLRDEVLAELLAIIDPVDYSDLESLRSEIIELVEQRI
                     EQKEWLAWIPATRPFFFLRSQIVVFDTGKRAETVEELATLLPELSPGSVYYHVVDARR
                     RFPVGLDDFSAWLEGLGPEYEPLRESLMEIDPYFGSLTDLRSHLCEAFNSFLEGKR"
     gene            4137..>5618
                     /locus_tag="PKI05_02795"
     CDS             4137..>5618
                     /locus_tag="PKI05_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOA51156.1"
                     /translation="MPRYNPSVIEPKWQQFWEKNKTFACPRLPTGPKFYVLDMFPYPS
                     GDGLHVGHPEGYTATDIMARYARMRGKTVLHPMGWDAFGLPAEQHAKKTGEHPRKATE
                     RNIANFRRQLKMIGFSYDWDREFATTDPDYFRWTQFIFLILYDTWYDPEQSRGRPIAE
                     LPIPPEIAEQGEDAIERYRDEHRLAYQLDAPVNWCPALGTVLANEEVVGGVSERGGHP
                     VVRIPLRQWMLRITAYADRLERDLDLLEWPESIKTLQRNWIGRSVGAEVDFFIGTFWH
                     KPGIPSQREYEGWRRDRELAGFPRKPDEACLRVFTTRPDTLFGATYMVIAPEHPFVER
                     LTLPEYREAVEAYREQAARKSDLDRTELSREKTGVFTGSYAINPVNGQLIPIWIADYV
                     LATYGTGAIMAVPAHDTRDFEFAKQFNLPIIPVVDPGDHASVSRQDVLEGKVAFVEEG
                     IAINSGPYNGLPTNEFKKRITEDLSEQGVGRQAINYRLRDWIFS"
BASE COUNT         1355 a         1618 c         1389 g         1256 t
ORIGIN      
        1 ccaacgacaa cacgtccccg accgcgccat gtgacatccg gcatcaatgc ccgaaagcac
       61 tctatcacat ttctcctatc ccgcgcggtc tgcacaccca aaagatcgta ttggagcaat
      121 gccctgagaa cctccaaacg ccacggcaac ttgagaaaga catcaagagg tggaaacgga
      181 atatggagga aaaaggcgag atgcgattgg actcccaaat cgcgcaagct ctgagcgacg
      241 agcataagct gataatcatg aatccaaata aaatgatccg attgcgaatg gctcgcaatg
      301 atttcggcat atttccgatt gacgcttcga taagcatccc agtaactggg tttaaattgg
      361 gccaggctag ggaattcatg gaacaacggc caaatgacct cgtttgagaa cccctcgtaa
      421 aacaagcgtt gctcacgtcg cgacaaggct accgggacaa tgcgataacc gaggtccttt
      481 ccagccttgt ccattaactc tgaaagattg cccgacgatt tctcagcagg gacgcccggc
      541 caaccgatcc atgcaccgcc gcggttgcgc aaaacggggg ccagcgctgt cactagcccg
      601 ccacttcctc gttccaacct ccacgtgccc tccctctcct tgacgacccg cagtggcaaa
      661 cgattggaaa caatcaacaa tcgttgagcc gttttactcg tcatgatcgc atcctcctct
      721 cactgcatct cgccactgct cgagaaaaag gcgaacctcg tcgccggtct cgaggcggac
      781 gtcagcgaaa gtcaacctgg gtgtcgggct aacaagaaca ttcagcccgc gctcacccag
      841 ctcctgaaaa gcgtcctcat ctgtttgatc atcgcccatg taacacaccg cgatgtcggc
      901 tcccttttcc tcatccaaga tcgttgatac cacacttccc ttgttgggac ctggcgcttg
      961 aagttctaca cccatatgaa atgctttcac catcaacacg ccgtgtttgt cgaatctggc
     1021 tcgcagttca tccacggtct tttgaagcca ttccgcatcc gtctgagaca gtccgcgcca
     1081 atgaatagca aggcaaccga ccttgcgttc taatcgcttt ccgggtgcca atcgtcggag
     1141 ttcgttttcg attctgtcca gaagatgttc cgtttccgca ttgggtttcc aaacccacct
     1201 tgttccattt ggctggatgc gttcgcgacc atgacttccc cagatttcaa tcgcttccaa
     1261 atgcaagagc ttcaagagat catccgcggc ccgtcctgag acgatcaaga ctcttgttcc
     1321 ccaggccgcg atatccccca aaatgccagg aacccatgtg taaggccggg cctcgctggg
     1381 gtcctcccga aacggcgcca gcgttccatc atagtcacaa agcaaaacac ctctcgtccc
     1441 cgcccgcaac cgtttgaagg ttgaagccaa tctgttggga tactttaaga cttccattga
     1501 gtcgtatcta ttcgttcgat ttcttgacgc aaaagcgaaa ccataagcgt caagtgatcc
     1561 ctcgccaaac gcgtcatgag aaagttttcg cggacatatt tctgcccctt ttcccccatg
     1621 cgccgggctt gctcgggtct ttcgaggaga taacggatcc tcatagcggc tccctccggg
     1681 gaacttacta aaaacccagt atggtgattg atgacctgta aacggattcc tcccgtattt
     1741 ccaccaataa cgggtttggc tttccacaga gcttctgtga cggtaagacc gaatccctca
     1801 cgcgtggact tctggaggac aatccatgcc gcccgttgca acgcattgat aattcgatgt
     1861 gcatcgctgg gaagctccaa aatatggatt gccgggtcgc ctgccgcggc tgcacgaact
     1921 tcagcgagaa cggcaccgcc ttctggatcg tcagtggctc ctcctcccgc caacacgagt
     1981 tgtgccgacg ggaaaaaact tcttaccagc cgaaatgcct taatcacgcc aatcgggtct
     2041 ttgaaccgat cgaagcgcga aacctgcaac agcaaagggc gatttagatc aagactgaaa
     2101 tccttgacaa caccttcaat aaagttctga gagagttcac agttcttccc actaaaagga
     2161 tcaatcgcgg gagcgatcaa gtactgggga tgcggcaacg cccgtgcaaa atcggcaaga
     2221 gaaaagatgc tcgcatcgta ctcctttaga aactggcgaa ggtaccgcca cacactgcgg
     2281 tcgggatggc tcacatcaat gtgacatcgc caaatccaaa tcccgtgtct ttcggggaaa
     2341 tatcggatga gggcggcggg ctgcgggtcg tggatgaaca ccacatcggc ctccgttaac
     2401 actgccttga gccgctctgc gttctccgca ttaatcttct cataaatccg aagatcctgt
     2461 tcgctcggtc gatctcccgc accttgaagg cagttgtgaa acttcttggt acaagcgaaa
     2521 aaatctggat ttccctcaat cgtgcaccat tcaacgtcca gccccagctc ttgcaacagc
     2581 ggaaccagtt ggctaaggat ttcggcgacg ccgcctccca ccctcgtcga attcacatgg
     2641 atgaccctaa tcccctggag cggttccgca agctgacgaa gctgcgttac cgcatcggca
     2701 cctgcaacgt cggcatatcg ttcgagaagt ccttccatca gcgttttcct tcgagaaaac
     2761 tattaaaggc ctcacataaa tgcgagcgca aatctgtcaa actgccaaag taagggtcaa
     2821 tttccattag cgattcgcgt aatggttcgt actccggccc caacccttcc aaccacgccg
     2881 aaaaatcatc caaaccaacg gggaatcgcc gccgagcgtc cacaacatgg taataaaccg
     2941 aaccaggaga gagttcaggc aataaagtgg ccagctcttc aactgtttcc gctcttttgc
     3001 ccgtatcaaa aaccacaatc tgggagcgca ggaagaaaaa cggccgggta gccggaatcc
     3061 aagccagcca ctctttttgc tcaattcgct gctcgaccaa ttcgatgatt tcgcttcgta
     3121 aactttccag atcgctgtaa tccacaggat caatgatcgc caataattcc gccaagacct
     3181 catctcgcaa atcgcgataa acccacacgg caaagtcatt tttgtattcc ggatcgtcaa
     3241 aacgagattg caacaaagca ccccaaaagt ggtggtaaat acactctggc ggcgccgcat
     3301 ttaaggcaaa cttgaattcg ctcaagttgc ttgccctcat tcccgtcgaa atcgggataa
     3361 gcgcgcaatc cttgataacg aaggtcatgg ccatctctcc ctgaaaacca agccatctct
     3421 cgcgatcgtc tcgcattctc tgcgaaaagg accacgtgcg atcgcgcacc aatgagtcac
     3481 tgccaccggg caaaccgtgc ggatgctcgc gtttcacaag ctccgttccg cacacggccc
     3541 gatgcgtctc cagccgtgat ctgcccgatt cgctctatct tgcggtgcca ccaatctgcg
     3601 aaagaagttt tgtcaatgta tgtttaccga tagaacagcc taagtcaacc cttttgacaa
     3661 aggtagcttg cgtaaatcgt ttatttatcg cagtagccgc tgctcaagta acgcctttgc
     3721 aatccgcacg agtcttgtcc gagccaacga accttttcga ttccaccgga gtacctcacg
     3781 ggctcagtct tcggcccctg acggcatcac ctcattcgga aggcgtctga acctttctcg
     3841 ctttccgact aaaaaggatc gcaattgggc tccgaaaaag cctttttaag aaccgattca
     3901 atacgaaaat cgcttagccc ttcgcgtgtc cgcttgaggc tccagatggt tttcagcgga
     3961 cttaagagac ctcggttcgc tctgttcgcc caagataacc cctttgcctg cgtgcactcg
     4021 aaaagctaga atccaagttt tggtagattg gcaaaaaatg tcgcggtgtg gcattttctg
     4081 gtaacgatcg tttccgatcg ctgagtttct ggtcggagtg ttcgcaagga gcgaccatgc
     4141 ctcgttataa cccttcggtt atcgagccaa agtggcaaca attttgggag aaaaataaga
     4201 cattcgcatg tcctcggctc cctactgggc caaagtttta tgtcctggac atgtttccct
     4261 atcccagcgg ggacgggctc cacgtcggcc atcctgaggg ttatacggcc acggatatca
     4321 tggctcgcta tgcccggatg cgagggaaaa ccgttctcca cccgatgggc tgggacgcat
     4381 ttgggctccc ggcagagcag catgccaaaa agacgggcga gcatccgcgg aaggccacgg
     4441 agcgaaacat tgccaatttt cgacggcaac tgaagatgat tggcttcagc tacgattggg
     4501 accgggagtt tgccaccacc gatccggatt atttccgctg gacccagttc atctttctca
     4561 tcctttacga cacgtggtac gatccggaac aaagccgggg acggcccatc gcagaactgc
     4621 ccattccgcc cgaaatcgct gaacagggtg aggacgccat cgaacgctat cgtgacgagc
     4681 accgattggc gtatcaattg gatgcccccg tcaattggtg cccggcgctg ggcacggtct
     4741 tggctaatga ggaggtcgtg ggaggcgtca gcgaacgagg gggacaccca gtggtcagaa
     4801 ttcccctgcg ccaatggatg ctccgcatca ccgcgtatgc ggatcgcctg gaacgagatt
     4861 tggatttgct cgagtggccg gaaagcatca agacgcttca gagaaactgg attggccgca
     4921 gcgtcggagc ggaagtggac tttttcattg ggacgttttg gcacaaaccc gggattccgt
     4981 cccagcggga atacgaggga tggcggcgcg accgcgaatt ggccggattt ccacgcaagc
     5041 cggacgaggc ctgtctgcgt gtgttcacga cgcggccgga tacgcttttc ggcgccacgt
     5101 acatggtgat tgcaccggaa cacccctttg tggaacgttt gaccttacct gaataccgtg
     5161 aggccgttga ggcttaccgc gaacaggcgg ctcgaaagag tgatttggat cgaacggaac
     5221 tttcccggga aaaaaccggt gtgtttacgg gctcgtacgc aatcaacccg gttaacgggc
     5281 agttgatccc gatttggatt gccgactacg ttctggcaac ttacgggacc ggggcaatta
     5341 tggcggttcc ggcccacgac acccgcgact ttgagtttgc caagcaattc aatctgccga
     5401 ttatccccgt ggtggatccc ggtgaccacg cctcggtgtc gcggcaggac gtgctcgagg
     5461 ggaaagtagc ctttgttgag gaagggattg ccatcaattc aggcccatat aacggcctgc
     5521 caactaatga gtttaagaaa cggattaccg aggacctctc tgaacaggga gtaggtcggc
     5581 aggcgatcaa ctatcggttg cgggattgga ttttcagt
//
LOCUS       DAOPTR010000089         5559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_11901_length_5559_cov_3.547057, whole genome shotgun sequence.
ACCESSION   DAOPTR010000089 DAOPTR010000000
VERSION     DAOPTR010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11901_length_5559_cov_3.547057"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..598)
                     /locus_tag="PKI05_02800"
     CDS             complement(110..598)
                     /locus_tag="PKI05_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51157.1"
                     /translation="MIRSQVAWEIGSKDNPVKMLDMVESDLTRVLSADVWFRSRLRYT
                     RWRNGQVVLRENCQIEALSLNSDVPLQTFALDGISFLEPGTEVFWNRKKPPPYTGRPL
                     IWDGQSIVPDPKYMVGEGARVQPGRVRWDVLIGANVALLAALATLVSLRRYFRLRKSP
                     SK"
     gene            complement(1559..1957)
                     /locus_tag="PKI05_02805"
     CDS             complement(1559..1957)
                     /locus_tag="PKI05_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51158.1"
                     /translation="MKTSVATLLTCLVAVCGMILIAWDASWVVGACSDTIVRPNSPSE
                     TTVQACPPAPISRNCPGSGTVVFTGFFQCDQPNAGTACQGAGVPSFTYCKKVCNCLET
                     VNPATGQLICDPSPVCEWFAAEQKREVACH"
     gene            complement(2274..2957)
                     /locus_tag="PKI05_02810"
     CDS             complement(2274..2957)
                     /locus_tag="PKI05_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51159.1"
                     /translation="MLRFLIVQMDVAIADGQWGLAKQTVLDLVTFAQTLSRTGDVTHF
                     EKAVTCCRAAARGVEQVLPHISPDEIRQLEKTLDDLKTQWGAVTQVVEAYRRRDTNRD
                     PNLAELQEGIGTYDVLRKRLETVAKKCQAFIVCAMAACRIYEKNHPMGDCSHVSTRVQ
                     NDAILNDLIDPFSGTGLRKKISSAGLVVYSVGPNLRDDGGVTGGDPGDIEATDTPDDI
                     AVTVRCGTE"
     gene            complement(3332..4309)
                     /locus_tag="PKI05_02815"
     CDS             complement(3332..4309)
                     /locus_tag="PKI05_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51160.1"
                     /translation="MDNKTHARIIEQLLDNIRKCESVTFTAVNTENLEYPDGRRVEIR
                     TVLRYWGKGDKFRAEVIDGTKGDAATPTFVTAYDGEKHQSVRHDLPSLGYSKESHLVN
                     PSITVNPLLLAFGWLHNPRSPVTWGEIRRRETWETAFARAVQRGRAVRENVECEIVDF
                     PGLIPNTINRVYFAREFRFFPMAAESIDQRSNEVFYNQHVSLYYVEEERVWIPAVIQT
                     SFDAAKLSHKVGGAVKSECVYRITPESVAVNIPLDDSLFILRAPPGGIVTDIDKVKKL
                     ANSDRVGKTSGRHFESSWTFLIGNVFLLGLVCVLLYVRWYWYRKRKVTT"
     gene            complement(4894..>5559)
                     /locus_tag="PKI05_02820"
     CDS             complement(4894..>5559)
                     /locus_tag="PKI05_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51161.1"
                     /translation="RWLLAGLFFVFAEYSLYRAAQGKASCGCFGAISVTPWLTFLLDS
                     VVVTSALWVKEVSTVKWPRKWSTWVCLMAGGTALWVGALVGASRLWPVEMAQDLGVRI
                     GDRRILLQPDAWVGRTFPLLSYMSGQPKEIQHGRWLVVLMNPGCGRCWMQLQAYEERA
                     EKEGTPLVVVWLSDWRSVISDGEVKVKEGTFWTTLEPMVEWLGETPVAIRLVDGRVES
                     VEH"
BASE COUNT         1425 a         1682 c         1145 g         1307 t
ORIGIN      
        1 ccatagcatc agtggactcc cattggcctg gaaaaatttg tcaaattctc ttgtaacgtc
       61 ttgcctacct tggagctcaa gaattctgca gcacaaatcg cgaatcactt tatttgctgg
      121 gtgatttccg caaacgaaaa taccgacgta gtgatacgag agttgccaga gcagccaaca
      181 gggcaacgtt ggccccaatc aacacatccc accggacacg acccggctgg accctcgccc
      241 cttctcccac catgtatttt ggatcaggaa caatactctg accgtcccaa atcaatggtc
      301 ttccggtgta gggtggaggc ttcttgcgat tccagaacac ttccgtacct ggctcaagga
      361 acgaaatgcc atctaaggca aatgtttgaa gtggcacatc actgttaagg gataaagctt
      421 cgatttggca attctcacgc aaaacaactt gtccattccg ccatctcgtg tatcgcaagc
      481 gagagcggaa ccagacatct gccgataaga ctcttgtcaa gtccgactcc accatatcaa
      541 gcatcttcac cgggttatct ttagaaccaa tttcccacgc aacttgagag cggatcactc
      601 caaacccacg tcgaggcata acaacaatgg aaaactccgc tggaagttgg ttatcagaag
      661 ccacatccga cgaccaacgg actgtccatt tctcttgctc gccctcacct tcccgaacta
      721 ctgatgggct tgccttcccg gtcgaagaaa aaaacacgtc gggatgacat acatgcattt
      781 gaagaatggg gtacgggaag catccgagcc accttgggtg cggaacgaaa aactgcctag
      841 gatgtatttc atctctatcc cagattgaca gccccatctt ggctccattg ggtagcgatg
      901 gatgcccgac gctataggca atgaaagcgt catcttcgta acacccccaa caccacacct
      961 cacgaaagtc tcctgcttct ctttcagccc gaagccggtc agctgagaaa tgtataagcc
     1021 aggtattagt gagagacact tttcgttcca aatccaaaaa accttgctcg atccgcacca
     1081 ccccagatac tattttggtg cgggaatctc tcacatcctt tagaacgcgt ttcaaatcta
     1141 aagattcact tgctttgcac atcccatgta tgaacaaggg actgagaaga attaccaacc
     1201 ctattcgtaa cccccgcgga cagttgaaca tgtcggcgct tctccgcccg gaactacatc
     1261 tgtttcgcct tggctttctg cacctttatg gtactcagga gcacattggc acttgtgttg
     1321 tccgccaaaa caaatctgag tcccactcaa atcgtcacac aagccgcttc aaaggcggta
     1381 atctgccctt tccgcgagcc attttgcaca cgcgtcgtaa acattaccaa ccaagcgtca
     1441 tagcgacttc gccattgcaa catattaggc ttgccacgcg agatctatcg tgcccgaacc
     1501 aaattcaaca ttgacggttg cgcattcctc aagaatccat gctgatagct tcgatttatt
     1561 aatggcaagc tacttcgcgt ttttgttcag cggcaaacca ttcacacacc ggcgacggat
     1621 cacagattag ctggccggtg gcgggattta cagtttccag acaattacaa acttttttac
     1681 aataggtgaa actaggaacc ccagctcctt ggcaagcggt tcctgcattc ggctgatcac
     1741 actggaaaaa tccggtgaag acaactgtcc cgcttccggg gcagttcctt gaaataggtg
     1801 cgggtggaca ggcctgaacc gtcgtttcgg acgggctgtt tggtctgaca atcgtatccg
     1861 agcaggcccc aactacccaa ctggcatccc acgcgataag aatcattcca caaaccgcaa
     1921 ccagacaggt cagcagcgtc gccacagaag tcttcatagc caactcctcc tcacatcaac
     1981 aacaaaaacc ttctactaaa actcaggcgc acgcaaccgc tcatggcttc tcatttcaag
     2041 aaccactaaa ccactcctaa taactccgat ctcctctcac tatccagctc atcacggatt
     2101 cagcacttgt tcccataatc tcggcgcgtg tccaaagacc tttagctgac agttctccaa
     2161 aacaccgtcc tggagcagcg taccaaccgc gattcgcatt tttagatcac ctcttgacga
     2221 tatgtgagca ctccattttg tcaggggacg agcgatgcga tccccccgcc tccctactcc
     2281 gtgccgcacc gaacggtcac ggctatgtcg tctggcgtgt ctgtcgcctc aatatcgccg
     2341 ggatcgcctc cagtaacgcc cccatcatcc cttagattcg gacccacact gtataccacc
     2401 aaccccgctg aagatatctt cttgcgtaat cctgtgcccg aaaatggatc aatcagatca
     2461 ttaagaatgg catcattctg aacgcgtgtg ctcacatggg agcaatcgcc catcgggtgg
     2521 ttcttctcgt aaatgcgaca ggccgccatc gcgcaaacaa taaaagcctg acattttttc
     2581 gccacagtct ccaaccgctt tcgtagcaca tcgtaggtcc caattccttc ttgcaattcg
     2641 gccaaattcg ggtctcgatt cgtgtccctg cggcgatagg cctccaccac ctgtgttact
     2701 gccccccatt gagtcttcaa atcgtctaac gttttctcaa gttgccgtat ctcatcgggc
     2761 gaaatgtgag gcaacacttg ctcgactcct ctcgcagcgg ctcgacaaca cgtcactgct
     2821 ttctcaaagt gagtaacatc gcccgttcga ctcaatgttt gagcaaatgt tactaagtcg
     2881 agtacagttt gcttagccag cccccattgc ccgtcagcaa tggccacatc catctggaca
     2941 ataaggaacc gtaacacgag ttgcacctgg gagatctctg cattctccca agcttctggc
     3001 tcaccaagga gtcacgtatc tcttgtgtct tcaccggaat tctgagtagc accttctgct
     3061 tttccgacag tttgtccaag tcgatggtgc gaagcggagc caagcgctgt gtgacagcgt
     3121 acagtcggtc atgagttgcc cgttctccat tcgcacggct caccccacgt ttgtcaaccg
     3181 atagagaact cgcggagtcc ttgcgacacc cccagagcac cattccaatg ccaaccatca
     3241 ctccgatcga aagcaaggac cttgatagag ccctccacat cgtcaattcc ttagtctgga
     3301 acattgcctt ttatcgacca cccattgttt atcacgttgt aacctttctt ttgcgatacc
     3361 aataccaccg gacatagagt agtacacata ctaaaccaag caagaacaca ttccctatta
     3421 agaaagtcca cgaactttca aaatgtcgtc ctgacgtttt accaacgcga tcggagttgg
     3481 caagcttctt cactttatct atgtcagtca ctattcctcc gggcggcgca cgcaaaatga
     3541 ataacgagtc atcaagcggt atatttacgg ccactgactc gggtgtgatc cggtacacgc
     3601 actcgctttt cacagcgccg ccaactttgt ggctgagctt ggcggcgtca aaagacgtct
     3661 gaataacagc gggaatccac accctctctt cttcaacata gtacagcgat acgtgttgat
     3721 tgtaaaaaac ttcgttactg cgctgatcta tagattcagc agccatggga aaaaaacgaa
     3781 actcccgcgc aaaatagaca cgattgatcg tgttaggaat caatccggga aagtcaacta
     3841 tctcgcattc tacattttct cgaacggctc gcccccgctg cacagccctc gcaaacgcgg
     3901 tttcccatgt ttcccgccgc cgaatctctc cccacgtcac tggactgcgg ggattatgca
     3961 gccatccaaa cgctaatagt aacgggttca cagttatcga cgggtttacg agatgacttt
     4021 ctttggaata gcccagcgag ggtaaatcgt gtcgcacact ttgatgtttc tctccatcgt
     4081 acgccgtcac aaaagtgggt gtcgccgcgt ctcccttggt tccatcaatc acctcagctc
     4141 gaaacttatc gcccttcccc caatatcgca aaacagtccg tatttccacc cggcggccat
     4201 ccggatactc gaggttttcc gtgttcacgg cagtaaatgt gactgattca cacttccgta
     4261 tattatcgag cagttgttca ataatgcggg catgcgtctt gttgtccata ccagacccca
     4321 tccctgatgc caaagcgatt agaaagcatg acagatagag aaaaggggtc atttgtgcaa
     4381 acgctcccgc cctgggttgt cctcctcaca attgggtttc cccaagaatc ttctaacgag
     4441 cttgatccgc tccacaaaca gggaaaccgc agcttcccgc caaaggccgt catgggggat
     4501 caattacaat caggcgaaca agcagtgccg ccacaatatc cgatccaccg gctgcacgga
     4561 tagaacggag cgagaaactc gaaacgagcc ccacactgcg ccggatcgtt cacatatttg
     4621 cagcccgttg attcataatc ccggggtaat tgtcccgtct gaaaccctcc agcattcatg
     4681 caacattccc caaaaagaga atagaacgtg acgcacggtt tcatcacatg cgtacatggc
     4741 ccctgtgcac gagctgtcgc aagcatcagc actcctgaca ttccaacaac accaaaaatc
     4801 gtagcacata tgaaagcttc tcgaacgcgc acactcatgg cttactcctc cacttgaata
     4861 aaaagttact ccttcccgga caatcccaac aaactagtgc tcaaccgatt cgacacggcc
     4921 atcaactaag cgaattgcaa ccggagtctc ccccagccat tcaaccatcg gctcgagtgt
     4981 tgtccaaaat gttccctcct ttactttcac ctcgccgtct gagataacac tccgccaatc
     5041 agatagccac actaccacaa gtggtgtccc ttctttttct gcacgttcct cgtacgcctg
     5101 caactgcatc cagcaccgac cgcaccccgg attcattaga accactaacc accggccgtg
     5161 ttggatctct ttcggctgcc cactcatgta cgacaatagc ggaaatgttc ttcccaccca
     5221 ggcatcaggt tggagcaaaa ttctcctgtc cccaatccga actcccaggt cttgtgccat
     5281 ttccaccggc cacaaccggc tggcacccac gagggcaccc acccacagtg ctgtgccgcc
     5341 tgccattaaa caaacccagg tggaccattt tcttggccac ttcaccgtgg acacctcttt
     5401 cacccacaac gcgctcgtta ctacgacact atcaagaaga aacgtcagcc acggggtaac
     5461 tgaaatggcg ccaaaacatc cacagcttgc cttcccttgc gctgccctat acagagaata
     5521 ctcagcaaat acaaagaata gtcctgcgag aagccacct
//
LOCUS       DAOPTR010000090         5557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_11906_length_5557_cov_4.239913, whole genome shotgun sequence.
ACCESSION   DAOPTR010000090 DAOPTR010000000
VERSION     DAOPTR010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11906_length_5557_cov_4.239913"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..2071)
                     /gene="mutL"
                     /locus_tag="PKI05_02825"
     CDS             complement(176..2071)
                     /gene="mutL"
                     /locus_tag="PKI05_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOA51162.1"
                     /translation="MSRIKRLPQSVINKIAAGEVIERPASVVKELMENSLDAGASRID
                     VAVEKGGVDLIRVADNGHGIHADELELAVASHATSKIETAEDLCHVRTLGFRGEALAS
                     IAEVSHLRIRSRSSDSDMGGELFVEGGVIRQLVPCGCPVGTVVEVRNLFFNTPVRRKF
                     LRSTTTELAHISETVARIALAHPHVHITLMHNDRPVLQLPATDNLLERIAALYGRELA
                     EGLIPVSSEDGPVRLFGYVAHPRFARRHAKMQFLFLNGRPIRDRNLQHALNEAYRGLL
                     TVDRQPIAFLTLEMPPELVDVNVHPTKQEVRFLDGGRLYGQLLGTIRKEFLTTDLITR
                     LPAGTESDQQTADTPHDPRQELAIRRQIVDWAKGELDRQRAAIQTAGVSPLSYPTSEG
                     VSPVGDAPASQNVIAGEQPQAESPLAASQTNTASLGRPSPQGRLPMDQSPPIRALQVH
                     NRYLVTESEDGVVVIDQHALHERILYEQLRQNIAQGRLPSQQLLIPEPVDLTPVEASL
                     ALENRELLAELGLNVEPFGGNTVLVHSYPALLRTMAPGELLRAVIAALGESPRDVDRT
                     ILLEKILHSMACKAAVKAGEQLNPDEINALLEQSRQVLHSHHCPHGRPAMLVFTREEL
                     DRYFHRT"
     gene            complement(2213..3637)
                     /locus_tag="PKI05_02830"
     CDS             complement(2213..3637)
                     /locus_tag="PKI05_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOA51163.1"
                     /translation="MTERVTHLERSTKSQDTASAPPVKVLIIDNDQDHAETMAEALER
                     AGCVCRTATSGPEGAKLIREEEFDVIITDLVMNEIDGLGILTLAKQEQPETEVILVTG
                     HGTIQSAVTAMREGAFNYLLKPLDLAQLRAVVQKAAENVRLRRANVELHRRLDETFGF
                     EGIVGNSPQMRAVLEKVRRIAPTNATVLILGETGTGKELIAQAIHQNSPRKNKPFVAL
                     NCAALSENILESELFGHVKGAFTDACSDRVGKFEYAHGGTLFLDEVGDMPMATQIKLL
                     RVLENGEITRVGSNDPIKVNVRVLSATNQDLEALVEAGRFRRDLYHRLNVVSIRLPSL
                     VERSQDIPILTDHFIKTFSKKHGKQIKGMTPAARHRLQTHNWPGNVRELRNVIESMVV
                     VDYDGILDVDDLPEHLAGDNGRQVDPGNVSLAALVGRSMDELEKMFIAETLRATGGNR
                     EEAARMLGIGERTLYRKIKEYGLS"
     gene            complement(3606..4388)
                     /locus_tag="PKI05_02835"
     CDS             complement(3606..4388)
                     /locus_tag="PKI05_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA51164.1"
                     /translation="MGGEAMRTRDEPSQKLLDQYTELARLAGALAHEIKNPLSTIRLN
                     LELLAEDFAQPETPRERRILARIEMVQKECDRLQTILEDFLKFTRADRLNLEPANLNE
                     EIRRALRFFAARAKESGIEIIDFLSSDLPTVLLDREAFQAALLNLLINAQQAMPNGGE
                     LVIRTVPSPDGVVIDLIDNGCGMDAETLAHAFDAFYTTKRGGTGLGLPTARKIIEAHG
                     GSISIQSEVGRGTQVTIKLAIPRQIPGEVVTTHDRAGDASRT"
     gene            complement(4620..>5557)
                     /locus_tag="PKI05_02840"
     CDS             complement(4620..>5557)
                     /locus_tag="PKI05_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOA51165.1"
                     /translation="FPYTIRIISDILESNGSSSMATVCGATLGLMAAGVPIRNPVAGI
                     SIGLIKESEDNWLLLTDIIGDEDHFGDMDFKVAGTQNGITGIQLDLKVRSINETIVRA
                     TLAQAREARIEILRKMLSVISRPRQDISVFAPRLLRTKIEPDKIGALIGPGGKTIRGI
                     QEKTRSTIEVEDDGTVIIAASSLEDAQAALKQVEAVTATVEVGKIYDGRVTSIKDFGA
                     FVEILPGKDGLCHISELADEYVANVSDICKVGDTVPVKVIAIDDQDRVKLSRKSALRE
                     LAQKREGEGNVEPPPPPPPKRVPPAHRRQDRDRNR"
BASE COUNT         1124 a         1578 c         1624 g         1231 t
ORIGIN      
        1 atcgcttgcc ctcaccccaa ccctctcccg gagggagagg gggacttttt gggatggact
       61 cttagccacg cgattccccg accgtttcgt cccatcggca gcggtgggcc caccgacgag
      121 ttgttctcat gcacgatctt ctcatccaca atcgcgcgcc ggccaaaaaa aggacttatg
      181 tcctgtgaaa ataacgatca agctcttccc gcgtgaagac aagcatggcg ggccggccat
      241 ggggacagtg atgcgaatgc aacacctggc ggctctgttc cagtagggcg ttgatttcgt
      301 cgggattgag ttgctctccc gccttgacgg ccgccttgca cgccatcgag tggaggatct
      361 tttccaggag gatcgtccga tcgacatcgc ggggtgattc tcccaaggca gcgatgaccg
      421 cccggagcaa ttcccccggt gccatggttc gcagcaaggc ggggtaactg tggaccaaca
      481 ccgtgttgcc gccaaagggt tccacgttga gtcccagttc ggccaaaagc tcgcggtttt
      541 ccagggccag actggcttca acaggtgtca gatcaaccgg ctcagggatg aggagttgtt
      601 ggcttggaag gcgtccctgg gcgatatttt ggcggagctg ttcgtagagg atgcgttcgt
      661 ggagtgcgtg ctgatcaatc accacgaccc catcttcgct ttccgtgacg aggtaccggt
      721 tatggacctg gagcgcccgg atgggcggcg attgatccat gggcagacgt ccttgcggtg
      781 acggtctgcc aagcgaagcc gtgttggttt gtgaggcggc aagtggcgac tccgcttgag
      841 gctgctcccc cgctatcaca ttttggcttg ccggtgcgtc acccacaggc gaaacgcctt
      901 ctgatgtggg gtagctcaac ggagagacac ccgctgtctg gatggcggca cgctggcgat
      961 ccaattcccc tttggcccaa tccacgattt ggcgacggat ggcgagctct tgtcggggat
     1021 cgtgtggggt gtcggccgtc tgttggtcgg attccgttcc ggcggggaga cgcgttatta
     1081 aatctgtggt gaggaattct ttgcggattg tgccgagcaa ctgcccgtac agccgacctc
     1141 cgtctaagaa tcgcacctcc tgcttggtgg gatggacgtt gacgtccacc aattccggcg
     1201 gcatctccag cgtgaggaat gcgatgggct ggcgatcgac ggtcaacagg ccgcgatagg
     1261 cttcgttgag ggcgtgctgg aggttccggt cgcgaatggg acgcccgttg agaaaaagga
     1321 attgcatttt ggcatgccgg cgggcaaacc gagggtgagc cacatagcca aacaggcgga
     1381 ccggaccatc ttcgctagaa accgggatca atccctcagc caactctcgc ccgtagagtg
     1441 ccgcgatccg ctccaggaga ttatccgtcg cgggcagttg aaggacgggg cgatcgttgt
     1501 gcatcagcgt aatatggacg tgaggatgtg ccagtgcgat gcgggcaacc gtctcggaaa
     1561 tgtgcgccaa ttctgtcgtt gttgagcgga ggaacttgcg acggacaggt gtgttaaaaa
     1621 agaggttgcg gacctcgacg accgtcccca cggggcagcc gcaaggaacg agctgacgga
     1681 tgacaccgcc ttccacaaaa agctcacctc ccatgtcgga atctgagcta cggctccgaa
     1741 tccggagatg tgagacctca gcgatagaag ccaaggcctc gccccgaaaa ccgagcgtgc
     1801 gaacgtgaca gaggtcttcc gcagtctcga tcttgctcgt ggcgtgactg gcgaccgcca
     1861 gttccaattc gtcggcgtgg ataccgtgcc cattgtcggc cacgcggatt agatcgacac
     1921 cgcctttttc cacggcgaca tcaatacggg atgcccctgc gtcgagggaa ttttccatca
     1981 attccttgac cacgctggcc ggtcgctcaa tgacctcgcc cgccgcgatc ttgttgatca
     2041 cactttgggg aagccgtttg attcgcgaca tggacgttcc ttacactccg cagggccgat
     2101 ctttcaaaga agacgctctc aatgttgttt ggcttgaatc ctgaattcac atcaaccaaa
     2161 caaagccccg accgtgcgaa aagcctccga gtgcggggaa ggctctccct gcttatgaga
     2221 gaccgtactc tttgatttta cgatagagcg tgcgttcgcc aatgcccagc atccgggcgg
     2281 cctcctcgcg gttgcctccc gtggcccgga gcgtctccgc aatgaacatc ttttccaact
     2341 cgtccattga tcgccccacc aaggccgcca gcgacacgtt gccggggtcc acttggcggc
     2401 cattatcccc ggccaaatgt tcgggcaggt catccacgtc gagaatcccg tcgtaatcca
     2461 caacgaccat gctctcgatg acatttcgca attcgcgaac gttgcccggc caattgtggg
     2521 tttgcaagcg atgtcgtgcg gccggcgtca tccccttgat ctgtttgccg tgctttttcg
     2581 aaaacgtttt gatgaagtgg tcggtgagaa tggggatatc ttgggacctc tcgacaagac
     2641 ttggcaagcg gatgctgacg acattcaagc gatgatacag gtcgcgacgg aagcggccgg
     2701 cttcgacaag tgcttccagg tcttgattgg tggccgagag aacgcggacg ttcaccttaa
     2761 tcgggtcatt tgagccgaca cgagtgattt caccgttctc caagacgcgg aggagtttga
     2821 tctgagtggc catcggcatg tcaccaacct catcgaggaa gagcgtgccg ccgtgggcgt
     2881 attcaaactt gccgacacga tcagagcagg catcggtaaa cgcccccttg acgtggccga
     2941 acaattcgct ctccaggata ttttcactca acgcagcgca attgagggcc acgaaaggct
     3001 tgtttttgcg agggctgttt tgatgaatgg cctgggcgat caactccttg cccgttccgg
     3061 tctcgcccaa aatgaggacc gttgcgttgg tcggggcaat acggcggacc ttttccaaaa
     3121 cggcccgcat ctggggactg ttgccgacaa tcccctcaaa accgaaggtt tcatcaagcc
     3181 gccgatggag ttccacattg gcccggcgta agcggacatt ctccgcagcc ttttgaacga
     3241 cggcacggag ttgggccaaa tcgagtggct tgagcaaata gttgaaggca ccttctcgca
     3301 tcgcggtaac ggccgattga atcgtcccgt ggcccgtcac gaggatgact tctgtttcgg
     3361 gttgctcctg tttggccagg gtcaaaatcc ctaggccgtc gatctcgttc atgaccaaat
     3421 cggtgatgat gacatcaaac tcttcctcgc ggatgagctt ggccccttcc gggccggatg
     3481 tcgccgtacg acaaacgcag ccggcgcgtt caagggcttc ggccatagtc tcggcgtgat
     3541 cctgatcgtt gtcgatgatc aggaccttca ccggcggtgc ggatgccgtg tcttgtgatt
     3601 tcgtgctacg ttcgagatgc gtcacccgct cggtcatgag ttgtcaccac ttctccagga
     3661 atctgccggg ggatggcaag tttgatagtt acctgtgttc cgcgccccac ttcactttgg
     3721 atggagattg atccgccgtg ggcttcgatg attttacgtg cggtgggcaa acccaagcct
     3781 gttcccccgc gctttgtggt ataaaaggca tcgaaggcat gggccagtgt ctctgcgtcc
     3841 atcccacagc cgttgtcgat caggtcaatg accacgccgt caggcgaggg aacggtgcga
     3901 atgacgagtt caccaccgtt gggcatggcc tgttgggcat tgatcaggag attaaggagg
     3961 gccgcctgaa aagcctcccg atcaagcaga accgtgggca gatcacttga gaggaaatca
     4021 atgatttcga tcccggactc tttagcacgg gcagcaaaaa aacgcaaagc ccggcgaatc
     4081 tcttcgttca aattggccgg ttctaaattt aaccgatccg cccgcgtgaa cttcaaaaaa
     4141 tcctccaaaa tagtttgcag acggtcgcac tctttctgga ccatttcaat acgagccaaa
     4201 atgcgtcgct cgcggggggt ctcaggttga gcgaagtcct ctgctagcag ttccaggttc
     4261 aggcggatcg tggaaagggg gttcttgatc tcgtgggcca gcgcgccggc caaccgggcg
     4321 agctccgtat attgatcaag gagcttttgt gaaggctcgt cgcgagttcg cattgcttcg
     4381 ccgcccattg ataaaccccc aaaatcctct tacgtgctac gacctaagtc gtacccgacc
     4441 gatccgtttt tcctgccgac gcccggactc ctgcaaacga ttatcttagc agaaatcccc
     4501 tcgattgatg agatccaagg ggaatgagca cggaatcccg aaatctcggc gcagaacgat
     4561 cgaacccaga acgatccaac tacgagcgac tggctttgcc gttgctacgc cgtgatgggt
     4621 tagcggtttc ggtcgcggtc ttgccgtcga tgggcaggcg ggaccctctt agggggtggt
     4681 ggcggaggcg gctcgacgtt accttcgcct tcccgtttct gcgcaagttc ccggagggca
     4741 cttttgcgac tgagcttgac ccgatcctga tcgtcaatgg caatcacctt gacgggcacc
     4801 gtgtcaccga ccttgcagat atcggagaca ttagcgacgt attcgtcggc cagttcgctg
     4861 atgtgacaga gtccatcttt tccgggcaag atttcaacaa aggctccgaa gtctttgata
     4921 ctggtgaccc gaccgtcata gatcttgccg acctcaactg tggccgtcac ggcttccact
     4981 tgcttgaggg ccgcctgagc atcttccaaa ctcgaggcgg caatgataac agtcccgtcg
     5041 tcttccactt cgatagtgga gcgggtcttt tcctggatgc cacggatggt cttgccgccc
     5101 ggtccgatca aggcgccgat cttgtcgggc tcgatcttcg ttcgcagtaa acgcggggcg
     5161 aagaccgaaa tatcttgccg gggccgcgaa atgaccgaga gcatcttccg caggatttcg
     5221 atgcgggcct cgcgagcctg ggccaatgtc gcccgaacaa tcgtctcatt gatcgagcgg
     5281 accttgaggt ccaattgaat gcctgtgatc ccgttctggg ttcctgccac cttgaagtcc
     5341 atgtcaccga agtggtcctc atcgccgatg atgtcggtga ggagcagcca attatcttcc
     5401 gactccttga tcaggccgat ggaaatacca gcgaccgggt tgcggatggg cacacctgcc
     5461 gccatgaggc ccaacgtcgc cccgcacacc gtggccatcg aactggaacc gttcgactca
     5521 aggatgtcgg agataatgcg gatggtgtag gggaaga
//
LOCUS       DAOPTR010000091         5548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_11931_length_5548_cov_2.951029, whole genome shotgun sequence.
ACCESSION   DAOPTR010000091 DAOPTR010000000
VERSION     DAOPTR010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11931_length_5548_cov_2.951029"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..2434
                     /locus_tag="PKI05_02845"
     CDS             203..2434
                     /locus_tag="PKI05_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51166.1"
                     /translation="MRLTLRTLLAYLDNILDPEDAAELRRKIEESDVAKAVLRRIREL
                     LRKERLGAPDVNDRQESLDPNTVAEYLDNTLPAERVADFEKVCLESDIELAEVAACHQ
                     ILAMILGEPAEVDPKTREKMYRLPQTSALSEKPLEKIVKELVAEPPAEEAARAASTKP
                     ETGKTPPAEPVTFVSPTPHVRSWLLGLMVLLVVGAIGVGALALYKTLLPAPPGLPGGE
                     VAQQTQPVKPHVDRGKSGVPDGGAPAKEPRGSVEGATPESPPGTPEAQQQTAPSTETA
                     PTSSPPAPPKADAAEAVAQKPSEQPPKTPEDESMVPPMEESPAPPVAEQAPDALAAVT
                     PQRGLPAPAEMLPSSPEIREVAPLPPEPIGQIVEDPKIPPILFRVHPDGALLHRVTLE
                     EPLRTSEVFFVPPKYRTTIRLADSVDVLCVGPVFWELLPMDANGQLGIGITFGQMILT
                     SRQADPTTLRLKCGSIEGRIEFGDAQSQVAVAVSRQPVCPGDPETQPVPWIVDMFSLG
                     GTARWFDAEHPRPPVLRAPVQMRLSEAELAATPMTDQPEWIKPRVTTATILEERAMAV
                     LEQALRDREKDPLLVLREQSANRQREVAWLAQRALAFAGEADVIWRVLEDLEYRPVWT
                     EAVAVLRELVRISPRHAAAVRAAAEKTLGMSGTLAYTLLWKYPDTGITKSQAEELVQQ
                     LEHNNLAVRVVAFWTLRHITGMTLNYEPHSLPNDRTRAVQLWRERLTKGSVRGVADER
                     PNL"
     gene            complement(2445..3380)
                     /gene="lpxI"
                     /locus_tag="PKI05_02850"
     CDS             complement(2445..3380)
                     /gene="lpxI"
                     /locus_tag="PKI05_02850"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF037151.2"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HOA51167.1"
                     /translation="MSLPLNDLIDPVSQSRHVSQKPLGLIAGWGRYPVLLAQRLSEQG
                     RPVYCLAVRDHADPALADYAERIVWLGLGQLGRAIRLFRRWGVEEATLVGKIHKVTLL
                     QPWMVFHHWPDWVGFRTFAPHFLHHVRDNRDDTLLTTVCNAFELAGIRIRPATDYLPE
                     ILISPGQITPQAPTPRQWADICFGWRVAKEMGRLDIGQTVVVKDQTVLAVEAIEGTDP
                     CLRRAGELCPVGGFTVVKVAKPQQDMRFDVPTVGVRTLENIASGKGSVLAVAAGKTIF
                     LDPDAVLSIALRHRIAIVAVTAEDIAAHAKDALPA"
     gene            complement(3394..4452)
                     /gene="lpxD"
                     /locus_tag="PKI05_02855"
     CDS             complement(3394..4452)
                     /gene="lpxD"
                     /locus_tag="PKI05_02855"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOA51168.1"
                     /translation="MPITLGELSDLVGGELDGPAHLVICQAKPLGEAGPTDITLIEKP
                     EKVAKISPGQIGAAVVPRSVTRANFPVIRVDDVHKAFGLIVRWFRPPRKLTRKGIHPQ
                     AIISPSAKIADDVEIGPFSVIGDDVEIGSGTTIYGGVHILSGCKIGRDVTIFPNVVLY
                     EDTIVGDRCIIHAGAVIGAYGFGYRLESGRYVLQAQLGNVILEDEVEIGANTTVDRGT
                     YGSTRIGQGSKIDNLVVIAHNCQIGRHNILCAQVGIAGSTTTGDYVVMAGQVGVRDHV
                     HIGERAVLGAMSGVINDIPPGARFVGIPATPEREQMLKQAAFTKLPEMRKQLKELQEV
                     VRQLTARLESLESSREAA"
     gene            complement(4716..4898)
                     /locus_tag="PKI05_02860"
     CDS             complement(4716..4898)
                     /locus_tag="PKI05_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51169.1"
                     /translation="MRQFLGKWLSVALFFVCLGVCSIQPGCKRTPPEPPALTEEVKSA
                     IQAEDQEIFEQEKAQR"
     gene            complement(5152..>5548)
                     /locus_tag="PKI05_02865"
     CDS             complement(5152..>5548)
                     /locus_tag="PKI05_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOA51170.1"
                     /translation="GSSNTILVAEKALNPKAAGSEGGDNERWNNAGWDEDIIRYGAGR
                     LSNGTEYGLPPIPDNTAPYNPGSGWTTIKDLGGRTWGQWHPFFGSSHPGGMNACMVDG
                     GVRFISFTVNADTFRRLSLTDDGETVDNF"
BASE COUNT         1200 a         1656 c         1566 g         1126 t
ORIGIN      
        1 tgtcttacaa aaatgggcaa cttaggggat tgtcaaagga tattggctta cactgccaac
       61 attttgtggt ccggcctgta cgaaaatcgg cgctttcggg cacaaatact gatggagggg
      121 tttcgcacca ctcctttcac ccaagacgta ttggctctgc cgaagataca ttgattactc
      181 ggtcaacagg ggacactgaa ccatgcggct aacactgcgg acgcttttgg cttatttgga
      241 caatattctc gatcccgagg acgcggcaga attgcggcgt aagatcgagg agagcgatgt
      301 cgccaaggcc gtgctacgcc ggattcggga gctcctgcgc aaggagcgcc tgggtgcgcc
      361 cgatgttaac gaccggcaag agagccttga tcccaatacg gtggccgagt atctcgacaa
      421 caccctgccc gccgagcggg tcgccgattt tgaaaaggtc tgtctggaat ccgacattga
      481 attggccgag gtcgctgcct gccatcagat tctggcaatg atcctgggtg agcccgccga
      541 ggtcgatcct aagacacggg agaagatgta tcgtcttccg caaacttctg ccctttctga
      601 aaagccgcta gagaaaatcg ttaaggaatt agtggcagag cctcccgctg aagaggcggc
      661 acgggcggct tcaacaaaac cggagacggg gaagacacct cccgcggaac ccgtaacgtt
      721 cgtctcgcca acgcctcatg tccgttcgtg gttgcttggc ttgatggtct tgctcgtcgt
      781 tggtgcgatc ggggttggag ccctggccct ttataagaca ctgcttcccg cgccgccggg
      841 cttgccaggt ggagaagtgg cccaacaaac gcagcccgta aagccccatg ttgatcgtgg
      901 caagagtggc gttcccgatg gtggcgcacc agcgaaagaa ccgcggggct cagtcgaggg
      961 agcaaccccc gaaagtcctc ccggcacacc tgaagcccag caacagacag ccccgtctac
     1021 cgaaacggct ccaacctcgt cacctccggc gcccccaaag gcagatgcag cagaagccgt
     1081 ggcgcagaaa ccatctgaac aaccgcccaa aactcccgag gatgaaagca tggtgccacc
     1141 catggaagag tccccggcgc cgcctgtggc agaacaagcc cccgatgcct tagcggctgt
     1201 cactccccag cggggtcttc ccgccccggc ggaaatgctc ccctcgtccc ccgaaatccg
     1261 cgaggtagcc ccgctgcctc cggaaccaat cgggcaaatc gttgaagatc ccaagatacc
     1321 cccgattctc tttcgggtgc atccagacgg tgcgttactt catcgcgtta ctctggaaga
     1381 acccttacgg actagtgaag tgttttttgt cccacccaag taccgcacta ccatccgttt
     1441 ggcggacagc gtggacgtcc tttgtgtcgg accagtcttt tgggaacttt tgcccatgga
     1501 tgccaacggg caactgggga ttggcatcac ctttgggcag atgattttga catcgcggca
     1561 ggccgatccc accacgcttc ggctgaaatg cgggagtatc gaaggccgga tcgaatttgg
     1621 ggacgcgcag tcgcaggtgg ccgttgcggt atcccgacag cctgtgtgtc ccggcgaccc
     1681 cgagacccag ccggtgccct ggatcgtcga tatgttttcg ctcggtggca cggcgcggtg
     1741 gtttgacgcg gagcatcccc ggccccctgt gcttcgtgcc ccggttcaaa tgcgacttag
     1801 cgaagcggaa ttggccgcga ccccgatgac cgatcaaccc gaatggatca agccgcgcgt
     1861 caccacggcg accatcctcg aagagcgggc catggcggtt ttagaacagg ccctgcgaga
     1921 tcgggaaaag gacccactac ttgtgcttcg ggaacaatcc gctaatcgcc agcgggaagt
     1981 ggcgtggctc gcccagcgag ccctcgcttt tgcgggcgaa gcggatgtca tctggcgggt
     2041 tttggaggat cttgagtacc gacccgtatg gacggaggcc gtagccgttc ttagggaatt
     2101 ggtccgcata tcgccccggc acgcggctgc ggtgcgggct gccgccgaaa aaactttggg
     2161 catgagcggc acgcttgcct acacgctctt atggaagtat cccgacaccg ggatcaccaa
     2221 gagccaagcg gaagaattgg tgcagcagct tgaacacaat aacttggccg ttcgcgtcgt
     2281 cgctttttgg acgcttcggc atatcacagg aatgacgcta aactacgaac cgcattccct
     2341 tccaaacgat cgcactaggg ccgtgcaatt atggcgagaa cggctaacga aaggctctgt
     2401 gcgaggtgtt gcagacgagc ggcccaacct gtgatcggcg tcactcaagc ggggagcgcg
     2461 tctttggcat gagccgcaat gtcctcggcg gtgacggcta caatggcgat ccgatgccgc
     2521 aaagcaatgg acaggaccgc atcgggatcg agaaagatcg tttttcccgc cgcaacggcc
     2581 aatacggaac cttttcctga ggcaatattt tccaaggtgc ggacgcccac tgtgggaaca
     2641 tcaaatcgca tgtcttgctg cggcttggcc accttgacga cggtaaatcc ccccacagga
     2701 caaagctcac cggcccgccg aagacaagga tccgtgcctt cgatggcttc cacggccaac
     2761 acagtttggt ccttgaccac cacagtctga ccgatatcga ggcgtcccat ttccttggcc
     2821 acccgccagc cgaagcagat atcggcccat tgccgcggcg ttggtgcctg gggtgtgatc
     2881 tgaccgggcg aaatgaggat ttcggggagg taatccgtag ccggtcgaat gcggatacct
     2941 gccagttcaa aggcgttaca tactgtcgtc agtagcgtat cgtcccgatt gtcgcggaca
     3001 tggtgaagga agtgtggggc aaacgtgcga aaccctaccc aatcaggcca gtgatggaag
     3061 accatccatg gttggagaag tgtcaccttg tggattttgc ccaccagcgt cgcttcttcg
     3121 acaccccaac gtcggaatag gcgaatcgca cggcccagtt ggcccagtcc tagccagacg
     3181 attcgttcag cataatcggc aagggcaggg tcagcgtgat cccgaacggc caggcaatag
     3241 accggtcgcc cctgctcgga cagtcgctgg gccagaagaa cggggtagcg tccccagcct
     3301 gcaatcaatc ccaagggttt ctgcgaaaca tgcctcgatt gcgacacggg atcaataaga
     3361 tcgttgaggg gaagactcat gagacgtcac ctgtcacgcg gcttcccgcg aggattccaa
     3421 cgattccagg cgggcggtca gctgacgtac aacttcctgc aattctttga gttgttttcg
     3481 catttccgga agtttggtga aggccgcctg cttgagcatc tgctcccgct cgggcgttgc
     3541 aggaatgccc acgaagcggg cgcccggcgg tatatcgttg atcacccccg acatggcgcc
     3601 cagcacggcg cgctctccaa tgtgcacatg gtcccgcaca ccgacttgtc cggccatcac
     3661 cacgtagtcc cctgtggtcg tgctgccggc gatccccacc tgagcacata ggatgttgtg
     3721 ccgtccgatt tggcagttgt gcgcaatcac cacgaggtta tcgatcttac tcccctggcc
     3781 gatccgtgtt gaaccataag ttccgcgatc gaccgtcgta ttcgccccga tctccacttc
     3841 atcttcaagg atcacattgc ccaattgggc ctggagcaca tatcgcccgg actccaaacg
     3901 gtaaccgaag ccgtaggccc cgatgacggc ccccgcgtgg atgatgcacc ggtcgccaac
     3961 aattgtgtct tcgtagagaa cgacgttagg aaagatcgtc acatcgcgac cgatcttgca
     4021 gcccgacaga atatgcaccc caccatagat ggtcgttccc gaaccgattt caacgtcatc
     4081 gccaatgacg gagaatggac cgatttccac gtcgtcggca atcttggcgg acgggctgat
     4141 gatcgcctgg gggtgaatcc cttttcgtgt gagctttcga ggtggccgga accagcggac
     4201 aatcaggcca aaggccttat gaacgtcatc caccctaatg acgggaaagt tcgcccgcgt
     4261 aacactgcgg ggaaccacgg cggcgcctat ttgacctggc gagatcttgg cgaccttctc
     4321 tggtttttcg attaaggtaa tatccgttgg cccggcctct cccaacggct tcgcctggca
     4381 aatcaccaga tgagccggcc catcgagttc ccccccaaca aggtccgaaa gctcccccag
     4441 agtgatcggc atgcgggatt cctttccatt aatgggcgac aaatcatcct tctcaatgtc
     4501 ggcaatcggg ccagcatgtg gatattatgc taacctttac cccggcctcc atgcaagatc
     4561 aaagctagct gccagaatgc ccgcatgcca atattctcct tgccccaaaa tggggaaccg
     4621 cagaaagaac cttcacgccc ccttgaatga tcgtccgtag gggccacgcc acaccgggat
     4681 gattactggg ggaggccgtc gccggaaatg agaaattacc tctgggcctt ctcctgctcg
     4741 aagatctctt ggtcctcagc ctgaatggcc gatttgacct cttccgtcag ggcaggaggc
     4801 tcgggaggtg tccgcttgca gccaggctgg atggaacaca caccaaggca aacaaaaaac
     4861 aacgcgacag acagccactt cccaagaaat tgcctcactc tttcgtctcc tcacaatcga
     4921 gcatgctgtg agctagtaag gtgaatattg ccaataacgt cagaattcag taggttaccg
     4981 ccgcatcggc caagaacctt cttcagcggt gggcattagg ggcgccgtca aaatcgtgtt
     5041 ttctgccaag agatcgcccg cagcacaggt ggttaaagcg actcgcgcgg gaacatcggg
     5101 tgacgcgctg cgggcgatcc ttggcatggc gggtaacaga gactctgctt ttcaaaagtt
     5161 gtcaacggtt tcaccgtcgt cagtgagact tagccgccga aacgtatcgg cgttgacggt
     5221 gaaagaaatg aagcggacac caccatctac catacaggcg ttcatacccc cgggatggga
     5281 cgatccgaag aaggggtgcc actgccccca tgtccgccca cccaagtctt tgatcgttgt
     5341 ccagccactg cccggattgt acggcgccgt gttatctggg attggcggaa ggccgtattc
     5401 ggtaccgttg ctgagccgtc ccgccccata ccgaataatg tcttcatccc agccggcgtt
     5461 gttccagcgt tcgttgtccc cgccttccga acctgccgct ttgggattaa gggccttttc
     5521 agccaccaag atcgtgttgg aggaaccg
//
LOCUS       DAOPTR010000092         5497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_12070_length_5497_cov_3.291621, whole genome shotgun sequence.
ACCESSION   DAOPTR010000092 DAOPTR010000000
VERSION     DAOPTR010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12070_length_5497_cov_3.291621"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..97
                     /gene="mraW"
                     /locus_tag="PKI05_02870"
                     /pseudo
     CDS             1..97
                     /gene="mraW"
                     /locus_tag="PKI05_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121784.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
     gene            251..850
                     /gene="recR"
                     /locus_tag="PKI05_02875"
     CDS             251..850
                     /gene="recR"
                     /locus_tag="PKI05_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOA51171.1"
                     /translation="MSELTASVAQLIECLTRLPGIGRKSAERIAYHLLRVSKEEAFAL
                     ADAIRAVKENVHHCKICFNLSEQETCHICRDATRDQTLLCVVEQPQDLMAIEQTGAYH
                     GLYHVLLGRLAPLDGVGPEQLTIRELVERVRNGTFREVIMATNPTVEGDGTALFISNQ
                     LADTGVKLTRLARGITPGSVIQFANKEILTDALTGRQVL"
     gene            1011..2633
                     /gene="rpoN"
                     /locus_tag="PKI05_02880"
     CDS             1011..2633
                     /gene="rpoN"
                     /locus_tag="PKI05_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOA51172.1"
                     /translation="MRMSLGHELRQVQKQVQKQILSPRMIQSMEILQLPLHDLEQRVE
                     QEVIENPALEMVEIGDDEGTEDEPLEMTDTALDEPVEETSEAVDGELEDAPVTRDEDE
                     AEEPTTGGGDLEREFVVDENNSAEEFSRLVEMEREIPEIFEEENSRPSRDRIEEVSER
                     HHDLIANIPDHTETLHDHLVNQLSWYDLDPATRAMAERIISNLDENGYLLTSLEDLLG
                     PFNSEEDRERAEKALSVVQKMDPPGVGARNLRECLLLQLDPENPYYHELKLMISEYLE
                     DLEHNRIPQIAKKSKLSLETIHAALQELRRLNPRPGAQFRNEPNMAVTPDVYVEETED
                     GRFIARVVSGRIPRLQLNRTLLESLNRGNLSKEEREYIKAKIDRAKWILDGIQQRKRT
                     LQRVAQAIVDHQYRFLKEGPESIEPLKMQQIADKLGIHVTTVSRAVDDKWIQTPQGIF
                     PLRRFFCGGTASSEGEEVAWDVVRLKVKELIEHEDKTKPLSDEEIVRELARQGIQVKR
                     RTVTKYREEMGIPSSRERRDWLAIERQKREKS"
     gene            2858..3916
                     /locus_tag="PKI05_02885"
     CDS             2858..3916
                     /locus_tag="PKI05_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOA51173.1"
                     /translation="MDNVSRQFLTQILGNPSPSGYEQPVQEVIRTYAREYADEVRTDL
                     HGNVIAVRNPKAPFRVLLAGHCDQIGLIVQYIDDDGYIYFQSIGGWDATVLIGQRVTI
                     WTKTGPIPGIIGRKPIHLLSEEERKSVPKLKDLWIDIGAANKAETTQRVRIGDPITIE
                     LAERELLNNRIVASGLDDKSGAWVVMEALKRVDPAKLRVAAYAVATVQEELGLRGART
                     SAFGIDPQVGIAVDVTFATDCPNMEKKEEGEIKLGRGPVIYRGPNMNPKVVDRLIELA
                     ESNNIAYQLAASGRATGTDANAIQINRAGVATGLVSIPNRYMHSPVEVISLDDLDAAA
                     NLLARFVEALNPDDDFTP"
     gene            4012..4770
                     /locus_tag="PKI05_02890"
     CDS             4012..4770
                     /locus_tag="PKI05_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOA51174.1"
                     /translation="MKRAIISDIHSNLEALEAVLKDIDSQGITEIYCLGDIVGYGPNP
                     RECIDLVMQRCKVCLLGNHDQGALFDPEGFNVGAEKAIFWTREQLEEHHGNPAESLKR
                     WDYLGERPRIHKEGPIVYVHGSVRNPLNEYVFPEDIYSPAKLQKLFSLIEKCSFQGHT
                     HTPGVFTEQCEFFSPEELPNGEFDLKSVSKAMINVGSVGQPRDGDPRACYVIQDEDRV
                     IFRRVPYNYELTCEKIYAIPELDNFLGDRLKEGR"
     gene            5168..>5497
                     /locus_tag="PKI05_02895"
     CDS             5168..>5497
                     /locus_tag="PKI05_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007340480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51175.1"
                     /translation="MMGDSQIRPERQNTASGGSAARTAGYRLEDQPTVITSGEILSPE
                     PATLETKGETISPTLSPGSTIGPYELLEFVGGGGMGRVFRAYDVRLSRIVALKVLTPE
                     QATDPQIL"
BASE COUNT         1387 a         1353 c         1585 g         1172 t
ORIGIN      
        1 ccgctctgac gaagaaaccg atacccccac agaaagtgaa atcgccgcta atccccgcgc
       61 acgaagcgca aaactgcgag cggcacgccg gacctagcgc ggttccccgg gggcccaacc
      121 gcagcgcgga gttctataat caacggctat cttgtagaat tcttttgcag gtgatgcagc
      181 ctgttccggg tggattgtgg ggggagtggg ctgccatcag gggggagcgc tttgaactgc
      241 gaggtgagac gtgagtgaat tgacggcatc agttgcccaa ctgattgagt gtttaacgcg
      301 acttcccggt atcggacgaa agtcggccga aagaattgcc tatcacctgc tgcgggtctc
      361 taaggaagag gctttcgctc tcgcggacgc gattcgcgcc gtcaaggaaa atgtgcatca
      421 ttgtaagatt tgcttcaacc tctccgaaca agagacctgt cacatttgtc gcgatgcgac
      481 aagggatcag actctacttt gcgtcgtgga gcagccccaa gatctgatgg ccattgagca
      541 aacaggggct taccatggtc tttaccatgt gctcctgggg cgactcgcac cactcgacgg
      601 cgtcggcccc gaacaactaa ccatccggga gttggtcgag agggtccgaa atggtacatt
      661 tcgagaggta atcatggcga cgaaccccac agtggaggga gacgggacag ccctgtttat
      721 ttctaaccaa cttgccgaca ccggtgtaaa actgacgcgt ttggcgagag gaatcacccc
      781 cggtagcgtt attcaatttg cgaacaagga aattttgacg gatgccttga cgggcagaca
      841 ggttctttga cgaaatggaa atggcaaaaa actgacgagg tcgttgccac ttttggtttt
      901 tgtgctgcgg aggtggtaga tttcaactga aggatgcatg cgtctgatct gggagaaacc
      961 ggattggacc gtactatggc cgagtttcca ctgcgtgtga ggtcacagtt atgcggatgt
     1021 cattaggaca cgaacttcga caggttcaga agcaagttca aaagcagatc ctgtcgccgc
     1081 gaatgattca gtcgatggag atcctccagc taccacttca tgatctcgaa cagcgcgttg
     1141 agcaagaggt tatcgaaaac ccagctttgg aaatggtgga aatcggcgac gacgaaggca
     1201 ccgaggacga gccgctagag atgacggaca ccgccctgga cgaaccggtg gaggagacct
     1261 cggaagcggt agacggtgaa ctggaggacg cgccagtaac tcgtgacgag gatgaagcgg
     1321 aagagccgac cacgggcggc ggtgacttgg aacgggagtt cgtggttgac gagaacaact
     1381 cggcagaaga atttagccgg ctcgtcgaga tggaacggga gatccctgag atttttgagg
     1441 aggaaaactc ccggccgtcg cgcgatcgga tcgaagaggt ctcggagcgt catcacgacc
     1501 tgatcgccaa tattcccgac cataccgaaa cactgcatga ccatttggtg aatcaattgt
     1561 cctggtatga cttggatccc gccacgcggg cgatggcaga gcgcattatt tccaatctcg
     1621 atgaaaatgg gtatttgctg accagcctcg aagacctatt gggtccgttt aacagcgagg
     1681 aggacagaga gcgggcggag aaggccctct ctgtcgtcca aaagatggac ccgccgggtg
     1741 tgggcgcgag aaacctccgt gagtgtcttc tgctgcaact cgatcccgaa aacccttatt
     1801 atcatgagct gaagctcatg atcagcgagt accttgagga tttagaacac aatcggattc
     1861 cgcaaattgc caagaagtcg aaactctctc ttgagacaat tcacgccgcg cttcaagagt
     1921 tacgccgact caacccccgc ccaggtgccc agttccgcaa tgagcccaac atggcggtta
     1981 ctcctgatgt gtacgtggaa gaaaccgaag acggacggtt tatcgcacgg gtggtatccg
     2041 gcagaattcc ccggttgcaa cttaatcgga cacttttgga aagcctgaat cgcggtaacc
     2101 tctccaagga agaaagagaa tatatcaagg cgaaaatcga ccgggctaag tggatcttgg
     2161 atggtatcca acaaaggaaa aggaccctgc aacgcgtggc ccaggccatt gtggaccatc
     2221 agtaccgatt tttgaaggaa gggcctgaat caattgaacc cctaaaaatg cagcagattg
     2281 ccgacaagtt gggaatccac gtcaccacgg tcagtcgtgc cgtggacgac aaatggatcc
     2341 agaccccgca aggtatcttc cccttacgga gattcttctg tggcgggacg gccagttcgg
     2401 agggcgaaga agttgcctgg gatgtggtcc gcttaaaagt gaaagagctg atcgagcacg
     2461 aagataaaac caagccgctc agtgacgaag aaatcgtgag agaattggca aggcagggga
     2521 ttcaggtcaa gcggcgaacg gtcacaaagt accgagaaga gatggggatt cccagctcac
     2581 gcgaaagacg tgattggctc gccattgagc ggcagaaacg cgaaaagagc tgaatgtcac
     2641 gggcaagccc tcctgccgga agagatggac ggggctgtgg ccacgttctt tctctggctc
     2701 gcgaaaatcc gtcgtagtgc gtccgttggg agttgccaac cgccgcgtat cgtgttctat
     2761 aatacacgat tcggattcat gccctgggtt aaggaatgcc aagttcgtct cggccactaa
     2821 gcgtcacggg aagaaagaag aaattggagc gacatcgatg gataatgtct cacgtcagtt
     2881 cctcacacag attctcggaa atccaagtcc ttcaggctat gaacaacctg ttcaagaagt
     2941 cattcgaaca tatgccaggg agtacgctga cgaagtcaga acggatttgc acggaaacgt
     3001 aattgccgtc cggaatccca aagccccttt tcgagtcctt ttggctgggc attgtgatca
     3061 gattgggctg atcgttcaat acatcgacga tgatggctac atctatttcc agtcaatcgg
     3121 tggttgggac gcgaccgtgt taattggcca gcgggtgacg atttggacga aaactggccc
     3181 cattcccggc atcatcggtc gaaaaccgat tcatttgttg tcggaagaag agcggaagtc
     3241 ggtacccaaa ctgaaagacc tgtggattga cattggcgcc gccaataagg cagagacaac
     3301 gcaaagagtc cgtattggtg atccgatcac cattgagttg gcggaaaggg aactcctaaa
     3361 taatcggatc gtggcgtcgg gcctcgacga caagtctggt gcatgggtgg taatggaggc
     3421 cctcaaacgt gttgatccgg caaaactccg ggttgctgct tatgccgtgg ctaccgtgca
     3481 agaggagcta ggattacggg gtgcacggac aagtgccttt gggatcgatc cccaggtggg
     3541 cattgccgtc gatgttacct tcgccacgga ttgccccaac atggaaaaga aggaagaagg
     3601 cgaaatcaag ttggggcggg ggccggtcat ttatcgcggg ccgaacatga atcccaaagt
     3661 ggtcgatcgc ctcatcgaat tggccgagag caataacatc gcctatcaac tggcagcttc
     3721 agggagggcg acaggcacag atgccaatgc cattcaaatt aaccgggcgg gtgtggccac
     3781 cgggttggtg agcattccca accgctatat gcatagccct gtggaagtca tttcgcttga
     3841 cgatttggat gctgcagcca accttctggc tcggtttgtt gaggccctga atcctgatga
     3901 cgatttcact ccataatggt gagcttgtta gccgagttat aagcacggat ttggagattt
     3961 cggctgaaag aaaggtttag gtttcacgtc ataaagaaat ggtgaccatc ggtgaaacga
     4021 gcgatcatca gcgacattca cagcaatctt gaggcactgg aagcggtttt aaaggacatc
     4081 gattctcagg gaatcaccga gatttactgt ctcggcgata tcgtagggta tggtcccaat
     4141 ccgcgggagt gcatcgacct ggtgatgcaa cgctgcaagg tatgcctcct gggaaatcat
     4201 gaccaagggg ctctcttcga tcccgaagga ttcaatgtgg gcgccgagaa ggccattttc
     4261 tggacccgtg aacaactgga ggagcaccac ggaaatccgg ctgaatcgtt gaagcgatgg
     4321 gattatctcg gggaacgccc ccgcatccat aaggaggggc ccatcgtgta cgttcatggc
     4381 tccgtgcgca acccgttgaa cgaatatgtc tttccagaag acatctattc gccagcgaaa
     4441 cttcaaaagc tcttctcact gattgaaaag tgctcctttc aaggacacac gcatacgccc
     4501 ggtgtgttta ccgaacagtg tgaattcttt tcgccggaag aattgcctaa cggtgaattt
     4561 gatctcaaat ccgtttcaaa ggcgatgatc aacgtgggtt ccgtgggcca gccacgggac
     4621 ggggaccctc gggcgtgtta cgtgattcaa gacgaggacc gagtgatctt ccgacgggtg
     4681 ccctacaatt acgagttgac gtgcgagaag atctatgcca tcccagaatt ggataatttc
     4741 ctcggcgacc ggctgaaaga gggccgctag ccgaatcact ccaagaacct cgcgaaagag
     4801 acgaccgctg ttttctttgc ataccagggg caagaatttt acaacaagcg agcttggtat
     4861 aaagcagagc ggcttctact ccgatgtaac cgccgggcta ccggaaagaa gagtctgttt
     4921 gaccgggttt accttttgcg cttataatga ggtaatgctc ctcctaatag gggaggctaa
     4981 tagcagcgca agaggcttcg tacggaaggt gatagtcccg atccccagcc atttgtgggg
     5041 gatcatctag tccaaattgt ctagttcatt ggtaactttt gcttttccag aacgataaga
     5101 gggcagtggc gccggtaagg attctcatcc ggggctcgtg gagtcctgaa agagtcttga
     5161 gagactgatg atgggtgatt cccaaatcag accagagagg caaaacactg catccggcgg
     5221 tagcgccgcg aggaccgcgg gatatcggct ggaggatcag cctacagtta ttacgagcgg
     5281 tgaaatcctt tcccctgagc ctgctacatt ggaaacgaag ggcgaaacga tcagccctac
     5341 tctttcgccg ggaagtacaa tcggtcccta tgaactcctg gaattcgtcg gcggcggggg
     5401 gatggggcgg gtattccggg cgtacgacgt ccggctatct cgcattgtcg cgctcaaggt
     5461 cttaaccccg gagcaagcca ccgaccccca aatcctt
//
LOCUS       DAOPTR010000093         5494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_12086_length_5494_cov_3.379665, whole genome shotgun sequence.
ACCESSION   DAOPTR010000093 DAOPTR010000000
VERSION     DAOPTR010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5494
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12086_length_5494_cov_3.379665"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /locus_tag="PKI05_02900"
     CDS             <1..1296
                     /locus_tag="PKI05_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOA51176.1"
                     /translation="SPAVGQVVVPQPGTKMDSLAQTVKQTILRVVPPLIVGSLVLGIT
                     LLYGQWRLSQFKTGQVNRSVRVALIQGSEDIRLEDSEEKRERIHRHYLDLAFQAARSH
                     RDLDLMVWPETVYGGFLIDHDDSPAVPATWADSDMTPERFREQIGKAREHSQRSLETL
                     AQHLRCPLLIGVETVSYTRQGPRIFNSAAYVALCEKINASQGGRERFSDGTDVKEANE
                     ESGKKGDSTGQRYCYFGRYDKMHLVMFGEYVPFADVIPLLQRLTPLSTSTTPGRTPVA
                     FPVGSLLLSPNICYESVIPHLIRNQILTLRAQGQEPDILLNLTNDGWFWGSSELDMHL
                     ICGVFRALETRKPFLIAANTGFSAHIGPDGRIYQQGPRRDVAILVADVTRSPLRSLYL
                     DYGIIPNTLLVSFIVLLVIAKCLSASRTFQRKITFRRSA"
     gene            complement(1274..2365)
                     /locus_tag="PKI05_02905"
     CDS             complement(1274..2365)
                     /locus_tag="PKI05_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51177.1"
                     /translation="MKTRTGLNQSVSQNLRVVFQALLGLIGFFSLLCDVARAQAIEPR
                     IQLEVAVDPRLPPLTHHDWAKALSEVGVENLRVRTAYPNDTPNIQEIPSESVPTYQVL
                     GVVNAQNELVLPDARFTIRQIDQLKQWINDLRHQGPPQSRPKQVAFGLNIVQFEQVRQ
                     DMSRPVQASTKGRPRAELVETLVSSLSYPTVFDPQARQRLGRDLWEEELQGLTTGTAL
                     AYILRYDGLALVPQSTVNGIRYQIVIGHPNLTAWPVGWAPEESEAKVVPKLLETLTVN
                     IQDVPVKDVLQSVTQRLSLMYLLDHPALARFGIQIDKALVNIPQTKTTYSQIIKRSLF
                     QGRLKGEIRCDEAGKPFLWITTLKPIDET"
     gene            complement(2362..3783)
                     /locus_tag="PKI05_02910"
     CDS             complement(2362..3783)
                     /locus_tag="PKI05_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOA51178.1"
                     /translation="MSLFEKEDEQRRREVQPLAARMRPRTLDEFVGQEHFLGEGKILR
                     RLIRADRLGSVIFYGPPGTGKTTLARILATETKSVFQQISAVRSGVKELREIIDGAAR
                     RLLASGQKTVLFIDEIHRFNRAQQDVLLPHVEEGGLVFVGATTENPFFSMTSALVSRS
                     RVFEFLPLTPEHIKVIVRRALTDRERGLGNYEVIMEEEALEFLAQACEGDARRALSAL
                     EVAVLSSETFPLRLTRQLMEDSLQRKAIVYDRQGDAHYDTISALIKSIRGSDPDAATY
                     WLAKMLEAGEDVRFIARRLMILASEDIGNADPTALPLAVAAAHACEMIGLPECRLNLA
                     QVVIYLACAPKSNAVIKAIDEALEDIRSGRVLPVPVHLRDANYPGAKRLGHGQGYVYS
                     QEEPDAIAEQDYLGVDKTYYVPSDRGFEKELKARLEVIRERLGRANKKISDLPDASTS
                     SLPEGSSSPPRLRKARKTKENQS"
     gene            complement(3896..4573)
                     /locus_tag="PKI05_02915"
     CDS             complement(3896..4573)
                     /locus_tag="PKI05_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HOA51179.1"
                     /translation="MSPMPSLKEIYDRLYNAFGPQNWWPGETPFEVLVGAILVQNTAW
                     ANVEKAIRNLREEGLMCPKRLYRLKDAELEELIRPCGYYRIKARRLKNVLRYLLKRHD
                     GSLEQMFRLPIDQLRRELLEINGIGPETADSIILYAGNLPTFVVDAYTHRIFSRHGWV
                     DPEADYHTLKEFFESQLEPDAALYNEYHALLVRLGKDYCKKRPRCQACPLEIYLPDGQ
                     PYDAWLE"
     gene            complement(4736..>5494)
                     /locus_tag="PKI05_02920"
     CDS             complement(4736..>5494)
                     /locus_tag="PKI05_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51180.1"
                     /translation="DAATLTGSEGIDTFVAGPRWADMTMPSTQIRIQKFASITANAGG
                     GWDTALLYDSPGDDHLRVWPRKAEMTGSGYQINLLNFEEITGKSSAGGTDIAELWDSP
                     GNDEFVSQPRESYLSGVGFINRVYNFATVHGYSTAGGIDVAYLDGGPNINAWVVRPDQ
                     TTVSSEDSYRRAKAFERVFALGGLDLCDTARLLDSTGNDELVIDSDQIVFRYPTSVVT
                     LSRIPKVTARTTGGIDRVVGRAVDVVLTLQGLWL"
BASE COUNT         1269 a         1483 c         1460 g         1282 t
ORIGIN      
        1 tcgccggctg ttggacaggt agtggtgcct cagcccggta cgaagatgga tagtctcgct
       61 cagacggtaa agcaaaccat cttaagggtt gtgccaccgc ttattgtggg aagcctcgtc
      121 ttgggcatta ctctgcttta tggccagtgg aggctatcgc agtttaaaac ggggcaggtt
      181 aaccggtctg ttcgcgtggc gttgattcaa gggtctgaag acatccgcct ggaagacagc
      241 gaggaaaagc gggagcggat tcatcggcat tacctggact tggcatttca agcagcccgt
      301 agtcatcgcg atctcgatct catggtttgg ccagaaacag tatatggcgg cttcttgatt
      361 gatcatgatg atagcccggc agtacccgca acatgggctg atagtgatat gacgccagag
      421 cgatttagag agcaaatcgg caaggcacgg gagcattccc aacggtcctt ggagacactc
      481 gcgcagcatt tacgatgtcc cctgttgata ggagttgaga ctgtctcata cacaaggcag
      541 gggcctcgca tcttcaattc tgcggcgtac gtcgctctgt gtgagaagat aaatgcgtca
      601 caaggcggac gggagcgatt ttcggacggt actgacgtca aagaagcaaa tgaggaaagt
      661 gggaaaaagg gagattcgac cggccagcgg tactgctatt tcggccgata cgataagatg
      721 catctggtca tgtttggaga atatgttccc tttgccgatg tcataccgct tctccaacgc
      781 ctgacccctt tgagcacaag cacgactccg ggaaggacgc cggtcgcgtt tcctgtcggt
      841 tcgctgctcc tatcaccgaa catctgttac gagagtgtca tacctcacct tattcggaac
      901 caaatattga ccttgcgggc ccaggggcaa gaaccggaca tcctgctcaa tttgaccaat
      961 gatggatggt tttggggttc gagcgaactg gatatgcacc tgatttgcgg tgtgttccgt
     1021 gccctagaaa cacgtaaacc ctttctgatt gcggccaaca cgggattttc agcccatatc
     1081 gggcctgatg gccgtattta ccagcaaggt ccccgccgcg atgtcgccat tctcgtggcc
     1141 gatgttacgc gcagtccctt gcgcagcctg tatttggact atggcatcat tcccaatacg
     1201 ctcttggttt cttttatcgt gctactcgtc attgctaagt gcctgtccgc cagtagaact
     1261 ttccaaagga aaattacgtt tcgtcgatcg gcttgagtgt ggtaatccat aaaaaaggct
     1321 taccggcctc atcgcagcgg atttcgccct tgagacgccc ttgaaacaga ctacgtttga
     1381 taatctgact gtaggtcgtc ttggtttgag gaatattgac gagggctttg tcaatttgga
     1441 tcccaaagcg ggccagggca gggtgatcca gtaaatacat tagactaaga cgttgtgtaa
     1501 ccgactgaag gacgtctttt acgggcacat cctgaatgtt aacggtcagc gtctccaata
     1561 gctttggcac aaccttcgcc tcagactcct ctggtgccca acccaccggc caggccgtga
     1621 gattcggatg accaatgaca atttgatagc gaatcccatt gactgtgctc tggggtacca
     1681 aggcaagacc atcatagcga aggatatagg cgagtgcggt tcccgtcgtt aacccttgca
     1741 gttcctcctc ccaaagatca cgtccaagcc tttggcgggc ctgcgggtcg aagactgttg
     1801 gataagatag gctggagact aaggtttcga caagttcggc ccgaggacgg ccctttgtcg
     1861 atgcttggac gggccgggac atatcctgcc gtacctgctc aaactgcacg atattgaggc
     1921 cgaaggcaac ttgtttcggg cgcgattgag gaggtccttg atgccgcaag tcgttgatcc
     1981 attgcttcaa ttgatcaatt tggcgaatcg taaaacgcgc gtcgggaaga acaagttcat
     2041 tttgggcgtt tacgacaccc agcacttggt aagtgggtac cgactcagat ggaatctcct
     2101 gaatgtttgg cgtatcgttg ggataagccg ttctgacccg aagattttcg acgcccactt
     2161 cactcaacgc tttggcccaa tcgtgatgcg tcaaaggcgg aagacgtggg tcaacagcaa
     2221 cttccaattg aatgcgtggc tcaatggcct gggcgcgagc cacatcgcaa aggagagaaa
     2281 aaaagccaat gaggccaagc aatgcttgga aaaccactct caggttttgt gacaccgatt
     2341 ggttaagacc cgtccgcgtt ttcacgactg gttctccttt gttttccgtg ccttgcgtaa
     2401 ccgtggcggc gaagaactgc cttcaggcaa agagctcgtg cttgcgtcgg ggagatccga
     2461 gatcttctta tttgctcggc ccagccgctc acgaatgact tcaagccgtg ctttcaattc
     2521 cttctcgaaa cctcgatcac tagggacata atatgttttg tcaactccca aatagtcctg
     2581 ctccgcaatg gcatcaggct cttcctggga atacacatat ccctggccgt gccccagacg
     2641 ttttgcaccg ggataattgg cgtctcgtaa atgaacgggt acgggcagga cacgaccgct
     2701 gcgaatgtct tcgagggcct catcaatcgc tttaatcacg gcattcgact tgggggcaca
     2761 agccaaataa atcactacct gggccaggtt gagacgacat tccggcaaac caatcatctc
     2821 acaggcgtgc gcagccgcaa cggccaaggg cagggcggtc ggatcggcgt tcccaatatc
     2881 ttcgctggca aggatcatga gacggcgggc aatgaagcgg acgtcttcac ccgcctccag
     2941 cattttggcg agccaatagg tcgctgcatc cgggtcactt ccacgaatgc ttttgatcag
     3001 ggcgctgatc gtgtcataat gagcatcacc ttgccggtcg tagacgatcg ctttccgctg
     3061 gagcgagtcc tccatcaact ggcgggtcaa acgaagggga aatgtctcag aagaaagaac
     3121 ggccacctct agggccgaca aagctcgtcg tgcatcaccc tcgcaggctt gggccaaaaa
     3181 ctccaatgcc tcctcctcca tgatcacctc gtagttgcca aggcctcgct ccctatcggt
     3241 gagtgcccta cgcacaatca ccttgatgtg ttctggtgtc agcggcaaga actcaaagac
     3301 ccggcttcga ctgacaaggg cgctggtcat cgaaaagaaa ggattctctg tggtggcacc
     3361 gacgaaaacg agtccgcctt cctcgacgtg gggtaatagc acatcctgtt gagcccggtt
     3421 gaaacgatgg atttcgtcga tgaaaaggac ggttttttgt cccgacgcca aaagacgacg
     3481 ggcggcccca tcaataattt cccgtagctc cttgacgccc gatcttacag cactgatctg
     3541 ttggaagaca ctcttcgttt ccgtagccag aatacgtgcc aacgtcgttt tgccggtccc
     3601 cggtggaccg taaaaaatga cggagccgag gcgatccgca cgaatgaggc ggcgaaggat
     3661 tttcccctcc ccaaggaaat gctcttgacc aacgaactca tccagtgtcc gcgggcgcat
     3721 ccgggccgcg agcggttgca cctcgcgtcg ccgttgttca tcttctttct cgaacaagga
     3781 catgggaaaa ccgccttaga tcacaagcca cgctgtcttc cggatgccca ttgatcccgg
     3841 atggaaacgg agttactcca tccaccattc cggcgccgct tgtccttccc tcgtgtcact
     3901 cgagccatgc atcgtatggc tggccatcgg gcaaataaat ctccaaggga cacgcctggc
     3961 agcgggggcg cttcttgcaa taatccttcc caagccgaac taagagggca tgatattcat
     4021 tgtaaagggc agcatcgggt tcgagttgac tctcgaagaa ttctttcaac gtgtgatagt
     4081 cagcttctgg atcgacccag ccgtgccggc tgaagatacg atgtgtgtat gcatcaacaa
     4141 caaatgttgg cagattaccg gcgtacaaga taattgaatc tgcggtctcc ggtcctatgc
     4201 cattaatttc cagcaattct cggcggagtt ggtcaatggg cagccgaaac atttgttcca
     4261 gcgacccatc atgccttttc agaagataac gcaaaacgtt ctttaaccgt cgggctttga
     4321 tccgataata accgcacggc ctgatgagtt cctccaactc ggcgtccttt agccgataaa
     4381 gcctcttggg acacatgagg ccttcctctc gtaggttgcg gatcgctttt tcgacattag
     4441 cccaggccgt gttctggacc aagatggcgc cgacgaggac ctcaaatggt gtctctccag
     4501 gccaccagtt ctggggccca aaggcgttgt acaatcggtc gtaaatctct ttcaatgaag
     4561 gcatcggact cataaatggc gcaccatgga ctcgagtgca gaacactcga gacgcatatt
     4621 tctcgtcggt tctctcaagg tgacaccggg gcgtgaacgc tgatcactct gcctggacat
     4681 aaccactaat tcacaacata gcggattgct gagaggtaag agaagtggtc ccggatcaca
     4741 accagagacc ttgaagggtc aagacaacat cgacggcacg accaacgacg cggtcaatgc
     4801 ctccggtggt ccgggcagtc actttgggaa tacgggacaa tgtgactacg ctggtgggat
     4861 aacggaagac aatctgatct gaatcaatca cgagctcatc gttgcccgtc gaatcgagaa
     4921 gtcgggcggt gtcgcagaga tcgaggcccc ctagggcaaa aaccctttca aaggcctttg
     4981 cccgacgata gctatcctcg gaggacactg tcgtttggtc gggcctcacg acccaggcgt
     5041 tgatgttcgg tccgccatcc aggtaggcca catcgatccc ccctgccgtt gagtagccat
     5101 gcacggtcgc gaagttatac acacggttga tgaatcccac tcctgacaag tatgattctc
     5161 gcggttggct cacaaactca tcattgcccg gggaatccca aagttcggca atgtcggtgc
     5221 ctccagcgga agactttccc gtaatttcct cgaaattgag cagatttatc tggtatccac
     5281 tcccagtcat ttcggctttt cttggccaaa cgcgcaagtg atcatccccg ggcgaatcgt
     5341 aaagtaaggc tgtgtcccaa cctccgccag cgttcgccgt gatggaagcg aatttttgga
     5401 tcctgatttg agtgcttggc atcgtcatat ccgcccagcg aggtcccgcc acgaaggtat
     5461 caattccctc ggagcccgta agtgtcgcgg cgtc
//
LOCUS       DAOPTR010000094         5465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_12170_length_5465_cov_4.144547, whole genome shotgun sequence.
ACCESSION   DAOPTR010000094 DAOPTR010000000
VERSION     DAOPTR010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12170_length_5465_cov_4.144547"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..1395)
                     /locus_tag="PKI05_02925"
     CDS             complement(4..1395)
                     /locus_tag="PKI05_02925"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POT family MFS transporter"
                     /protein_id="HOA51181.1"
                     /translation="MLKRLSSSLLENFKTKPQYPTAPIPTDGLPPGVPYIVGNEAAER
                     FSFYGMRAILTIFMTQYLLDAHGRLAPMSESEAKFYFHLFNAGVYFFPIVGAILADIF
                     WGKYSTIVGLSLVYCLGHVALAWDETRLGLFVGLTLIAVGSGGIKSCVSAHVGDQFAS
                     RNSHRMGEVFAWFYLAINLGAASSTLLTPWLLVVAGPGLAFAVPGILMALATAVFFAG
                     RAKFAHIPPSGKKFFYELISPEARSTILRLIPLFLCVAMFWALFDQSGSAWVLQAEKM
                     NRRWLGIQWESSQIQAVNPLLVMIMVPLSTYVIYPLVNRVWQFTLLRRVSVGMYVAAC
                     SFCVTAWIEHTIAAGARPSIGWQVVAYVVITAAEILISVTCLEFAYTQAPRTMKSVIM
                     GLYLLSVSLGNSFTAVVNKFIELTGGDRPGGWAQGPGYYWFFVLAMALTGVVFTIVSR
                     FYREKTYLQASET"
     gene            1802..1875
                     /locus_tag="PKI05_02930"
     tRNA            1802..1875
                     /locus_tag="PKI05_02930"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1836..1838,aa:Arg,seq:ccg)
     gene            2876..3181
                     /locus_tag="PKI05_02935"
     CDS             2876..3181
                     /locus_tag="PKI05_02935"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOA51182.1"
                     /translation="MKNEGMPGLIRELRQRLNLTQEQLAEKVGVASSTVNHGQNGKRV
                     PLPFLVKRLVELKYEFGRKLEESERYKAVKALARKTDHLLFLTAIRTEGEFRSPLWC"
     gene            3247..>5465
                     /locus_tag="PKI05_02940"
     CDS             3247..>5465
                     /locus_tag="PKI05_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOA51183.1"
                     /translation="MSTAYHAKYFAYELTRIGGTGMERLSRSLFDACVDLNPHQIEAA
                     LFALRSPISKGVLLADEVGLGKTIEAGLVLCQLWAERRRRLLVICPASIRKQWALELT
                     EKFNLPTVVIDAKEYRERQGRGMPNPFEADEVVVTSMHFASAHASDIRSAQWDLVVID
                     EAHKLRNAYRDSNRMGQNIRWALEDRRKVLLTATPLQNSLLELYGISTIIDDHIFGDL
                     PSFRSQFVNAGGNVEELRNRLRTFSIRTLRRQVVEYIQYTERRLITRPFKPTEQEHKL
                     YEAVSAFLQRTDTYALPYQQRHLTALIVRKLLASSPHAVAATLEAMRDRLIAIRDKLP
                     VPEDFAERLIADEEMEHELLDEMTDGLLQRSGDEDAEKRPQVTEAEQAEPVIDRQKLL
                     AEIEELDRYAQWARSIGIDTKTRSLIKALEIGFEKMTEMGAAQRAVIFTESRRTQAYL
                     KDFLEANGYAGRVITFNGSNNEPETTAIYERWVEANKETGRVTGSRSVDVRTAIIEHF
                     RDHGQILIATEAAAEGVNLQFCSLVVNFDLPWNPQRIEQRIGRCHRYGQKHDVVVINF
                     LNEKNEADRRVYELLQEKFSLFSGVFGASDEVLGSIESGVDFERKVLDIYQQCRTPDE
                     IEQAFNALRAELDEKIRSKLAETRQKLFEHFDEDVHAHLRLNLEGARQQLDRVGMMFW
                     RLTRFVLDGRATFDEANLAFDLKTPPHDAIQPGYYRLISKDHENVTGAFLYRLSHPLG
                     "
BASE COUNT         1267 a         1591 c         1570 g         1037 t
ORIGIN      
        1 cgtctatgtt tcagatgcct gaaggtaggt tttctctcgg taaaaccggg aaacaatcgt
       61 aaatacgacg cctgtcaacg ccatcgcaag cacaaaaaac caataataac cgggtccctg
      121 cgcccaaccg ccgggtcggt cgccaccggt cagttcaatg aacttattga ccacggccgt
      181 gaatgagttc ccaagcgata cggacagcag gtataaaccc atgatcactg acttcatggt
      241 acgcggggcc tgagtgtagg cgaactccag gcaggttacg gagatgagga tttccgccgc
      301 ggtaatcacc acgtaggcca caacttgcca tccgatgctc ggtcttgccc ccgcagcaat
      361 cgtgtgctcg atccaggctg taacacaaaa ggagcatgct gccacgtaca tcccgacgga
      421 aacacgcctc aaaagtgtaa attgccatac ccgattgact aaggggtaaa tgacataggt
      481 cgaaagggga accataatca tcaccagcag cggattgacg gcctgaatct gtgaggattc
      541 ccactggatt cctagccaac ggcgattcat cttctccgcc tgaaggaccc atgccgaccc
      601 ggattgatcg aacagggccc agaacatcgc cacgcataag aaaagcggaa tcaatcgcag
      661 aatggtggat cgggcctcgg gactgatcag ttcgtaaaag aacttcttac cgctcggggg
      721 aatatgggca aacttagccc gcccggcgaa aaagacagcc gtggccaatg ccatgaggat
      781 gccaggcaca gcaaaggcca agcccggacc ggcgacgact aacaaccacg gtgtaagtag
      841 cgtggaactg gctgccccaa ggttgatggc caggtagaac cacgcgaaaa cttcacccat
      901 ccggtggcta ttgcgacttg cgaattgatc cccaacatgg gcggagacgc acgatttaat
      961 tccccctgat ccaacggcaa taagtgttag ccccacgaat agccccaacc gcgtttcatc
     1021 ccaggccaag gcgacgtgtc ccaagcaata caccagagaa agccccacaa ttgtggagta
     1081 ttttccccag aagatgtccg ccaaaatggc ccccacaatc gggaaaaaat acaccccggc
     1141 gttgaacagg tggaaataaa atttggcttc actctccgac ataggggcca agcggccgtg
     1201 ggcgtctaaa aggtattgcg tcatgaaaat cgtcaggatg gcccgcatgc cgtagaagct
     1261 aaatcgttcg gcggcctcgt tgccgacgat gtagggcaca ccgggcggta gcccatccgt
     1321 cgggataggc gcggtcggat actggggctt cgttttgaag ttttcgagaa gggaggacga
     1381 aagccgtttg agcataaaat gatctctgcg tacttgccga gggcagactc tttggatgat
     1441 tccaaacgaa ttgagtgacc aaaatggtca gcttagcaaa ttgtccggaa gtcgccaaat
     1501 cccgcagctc catcagattg aaagcccctt ttcccaggtc cgcaaaagag aaccgagtaa
     1561 ctccccaagc catccattct gtcgcaggag ccccaagcac ctcggccccg caccactccg
     1621 gcaagtcccc tcgaatcggc ctttggcgac gcgttccaac tgctgtcctg aaatggttgt
     1681 ccagatatgc cgacgggcaa tcttcccaag aagacctcgg ttggcttaaa atggactact
     1741 agcagaccgc tgaggtgatg aacgtttcgc ttgtggtctg ctagacggtg ttgtgggtat
     1801 tgtccccgtg gctcaattgg atagagcgtc ggcctccgga gccgaaggtt acaggttcga
     1861 gtcctgtcgg ggacgcttct tcaggcctca cccttgcctc ggacgccgca cgtgctaaat
     1921 gcttgcggcg tcttgctttc tggccagaac tctagcccac ttcgccactt gtgtcggcca
     1981 cgcaatccga cgctgtgccg aactctcggc ccgcttcccg tgcaaatcgc tgcaaaccga
     2041 tgcaacgccc ggcaagcagt ctagcaccat agatggcacc tcgccggaaa agagagcacc
     2101 acgggaagca ccagtcggct gggcctacca cctaccttgg tctggcgggc cgtggcggtc
     2161 ggcccgtatg gcaagcacag ggcaccaact ggtgaggccc cacacggcga ttgccaacgg
     2221 ctccaaattc ggagtcgtcc gaatggttgg tgtctctcca tcgacatgac ctgcgcacct
     2281 ggataggaag ctactcgtca cacaccggcg catatacttt ccacgtggta tgttctcact
     2341 gtgctccgtt gctggggcgt gtaaccgctg acagcctgac acagggggaa cgccaagcca
     2401 caagcccgga tgataaagaa ccgcagtgag ggcacaatca agtgcctgat ggcgacaacg
     2461 agggcaggga gcaccgcgaa gaggagcttc gaccgttgcc aacaagaact agaaggctga
     2521 acggccttgc tgtgcgcagg aagtaatggc gatccaaatt tgctcgcacc gcgggcggct
     2581 tcactccagg tgccctgcgt tatccggcgg ttgatcaaca cgaagcgggc ggcttgctcg
     2641 gtcagcttgc ccgtcggctc aatacccggt gtggttaggg gtgaaaagtg gcttttgaac
     2701 aggcgtgtgg ggacctcggc atttgctctt cactggtgaa aagaggcaaa gtctctcgga
     2761 ccatctgagg ccattcaaaa cgttgacagc cgcgtcgcat gagggctatc atggcggcat
     2821 atatgcatat aaatattctt cgccgcgttc gcaaacgagg gtgcgagata tcaccatgaa
     2881 gaatgagggc atgccggggc tgatccgcga acttcgacaa cggctgaatc tcacgcagga
     2941 gcagctcgcg gagaaggttg gtgtcgcctc cagcaccgtg aaccatgggc agaacggcaa
     3001 gcgggtgcct ctcccgtttc ttgtgaagcg actggtcgag ttgaaatacg agttcggcag
     3061 gaagctggaa gagagcgagc ggtacaaggc ggtgaaggcc ctcgcccgca aaaccgatca
     3121 tttgctgttt ctcacggcaa tccgcaccga gggagaattc cgatctcccc tctggtgttg
     3181 atctggtaat atgagataca ggattgacga aaacgagagc aagagactga cggggaagac
     3241 gtagtgatga gcaccgccta tcacgcgaag tactttgcgt atgagctgac ccgcatcggc
     3301 ggcacgggga tggagcgtct ttcccggtcc ctgttcgacg cgtgcgtgga tctgaatccg
     3361 caccaaatcg aggcggccct gttcgccctc cgatccccaa tctccaaggg cgtccttctc
     3421 gccgacgagg tcggtctcgg gaagaccatc gaggccggtc tggttctctg tcagctttgg
     3481 gccgaaagac ggcgccggct tctcgtcatc tgccccgcct ccattcgcaa gcagtgggcg
     3541 cttgagctga ccgagaagtt caaccttcca acggtcgtca tcgacgcgaa ggagtaccgg
     3601 gaacgacagg gccggggaat gcccaacccc ttcgaggcgg acgaggtcgt ggtcacgtcc
     3661 atgcactttg ccagtgctca cgcgtccgat attcgttctg cccagtggga tttggtggtc
     3721 atcgacgagg ctcacaagct gcgcaacgcc taccgggact cgaatcgtat gggccagaac
     3781 atccggtggg cacttgaaga ccggcgcaaa gtgctcctga cggccacgcc gcttcagaac
     3841 tccttgctgg agctctacgg aatctcgacc atcatcgacg atcacatttt cggagatctt
     3901 ccgtccttcc gaagccagtt cgtgaacgcc ggtgggaacg tggaggaact gcggaacagg
     3961 cttcggacgt tctccattcg cactctccgg cggcaggtcg tcgagtacat ccagtacaca
     4021 gagcgccgtc tcattacgcg ccccttcaag cccacggagc aggagcacaa actgtacgag
     4081 gcggtgtcgg cgtttctcca gcgcacggac acctacgcgc ttccctatca gcaacgacac
     4141 ctgactgcat tgatcgtccg caagcttctc gcctcatcgc cgcatgcggt ggcggccacg
     4201 ctcgaagcca tgcgcgaccg actgattgcc atccgtgaca agctgcccgt ccccgaggac
     4261 ttcgccgagc gcctcatcgc ggacgaggaa atggaacatg agctgctgga tgagatgacc
     4321 gacggacttc tgcagcgttc tggcgacgaa gatgcggaaa agcgccctca ggttaccgaa
     4381 gccgaacagg cagaacccgt tatcgaccgc cagaagctcc tcgcggaaat cgaggagctg
     4441 gaccgctacg cccagtgggc gagaagcatc gggatcgaca cgaagacccg ctcgctgatt
     4501 aaggcccttg aaatcggctt cgagaagatg accgagatgg gggcggccca gagagcggtg
     4561 atcttcacgg agtcgagacg gacccaggcc tatctcaagg acttcctgga agcgaacggc
     4621 tacgcaggcc gcgtcatcac gtttaacggg agcaacaatg aaccggaaac aaccgccatc
     4681 tacgagcgct gggtggaagc caacaaggaa actggtcgag tgaccgggtc gcggtcagtg
     4741 gatgtccgta cggccatcat cgaacacttc cgcgaccatg gacaaatcct gattgcaacg
     4801 gaagcggccg ccgagggtgt gaacctccaa ttctgctccc tggtggtaaa cttcgacctt
     4861 ccctggaatc cccaacggat cgagcaacgg atcgggcgct gccatcggta cgggcagaag
     4921 catgacgtcg ttgtcatcaa cttcctcaac gagaagaacg aggccgaccg gcgcgtctac
     4981 gaactgctac aggagaagtt cagcctcttt agcggcgtgt tcggcgcttc cgacgaggtc
     5041 ctgggcagca tcgagtccgg cgtcgacttc gagcggaagg tgctggatat ctatcagcag
     5101 tgtcggacgc cagacgagat cgagcaggct ttcaatgcct tgcgggctga acttgacgag
     5161 aagattcgtt ccaagctggc cgagacccgg cagaagcttt tcgaacactt cgacgaggat
     5221 gtccatgccc atcttcgact gaacctggaa ggcgcacgcc agcagctcga ccgcgtaggg
     5281 atgatgttct ggcggctcac gcggtttgtt ctggatgggc gggcgacgtt cgacgaggca
     5341 aacctggcgt tcgacctcaa aacgcctccg cacgatgcca tccagccagg gtattaccgc
     5401 ctgatctcga aggaccatga gaacgtcacc ggagcattcc tctatcgcct gtcgcacccc
     5461 ttggg
//
LOCUS       DAOPTR010000095         5457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_12191_length_5457_cov_3.567753, whole genome shotgun sequence.
ACCESSION   DAOPTR010000095 DAOPTR010000000
VERSION     DAOPTR010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12191_length_5457_cov_3.567753"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(492..2765)
                     /locus_tag="PKI05_02945"
     CDS             complement(492..2765)
                     /locus_tag="PKI05_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51184.1"
                     /translation="MKRLRRFLEIIAACGLATVAAYWLAMSDVGPGAELVTHTKASTS
                     PAETALQTSPNREANSYSRPSNVEQLPSYEPGPQVYIQNKVQSEDKPNKAVAGTVVNP
                     FISNSPSTATAFPDRLVTTGYDLPARSEPDSVMVSPQETESRTIVPVNYEVDPSSPVA
                     DVGSPVRLRVTSLEESPDRGRVAPITYQEANPSAVSERGLSNVDDPTLQVPPGGNSEA
                     LSGLQSDAQGRSGPSQERSTQEVVPSPLPDIREMTINPPQAEITVNQAGIQLELRDAD
                     IRDAVHTLAKLAKLNVVMSSSVQGKVTVSLNTLTPRDAIFAVLRVPGFVSQEHEGVLY
                     IGTEQEIQNLGYITDQIATRIYRPNYTPAKELESLIKPLLTPQVGLITVSTAAENGIA
                     PDPSNAGGDRYAGTDVLIVRDYQRVLKEVDRVVQEVDVRPAQVAIEAMILSVNLDDKD
                     QFGVSFELLRNKGYIKLGWGLVPQSLSDIKFDNNGLKLAFLDGSIGAFLNALESIGDT
                     NVIATPRLLVLNKHRAEILIGKELGYVSTTFTETSSSQTVDFLKVGAQLVIRPFISND
                     GLIRMEVHPELSTGSVQITNNFTVPNKDTTQVTTNIMVPNGVTVVIGGLMREDLEKTA
                     SQIPFFGNLPLIGAAFRNKMETTRKQEIIVLITPYIIDDYRMATDGATAQASWQRRQE
                     VAGEEMSGLNKQNASKHFLEKAKTAYLQGRCDAAKYWAEMAVHFDPSNLEAIAFRDQL
                     AGVATQNTSATLRPSNR"
     gene            complement(2821..3468)
                     /locus_tag="PKI05_02950"
     CDS             complement(2821..3468)
                     /locus_tag="PKI05_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51185.1"
                     /translation="MSKWIKKIKREFKRSPGKAAVLGGLAVVAVAFWGYRLAAAPRSV
                     SQQASVSGMGQAETETDGPVFPTQEGSIVASSEEAKVDLTPWHVLQAWRAQPFQAETA
                     SAQLARDPFVMKSPPVHQSAEESLGKSDVDDVSPATLGLRFTGVVIAGDWRAAILENR
                     LLWEGSAFKVHRNGRSYVVRICEVTPTAIKLTIDGAVFEQSLPELGLSPKGSEAP"
     gene            complement(3540..4085)
                     /gene="pilO"
                     /locus_tag="PKI05_02955"
     CDS             complement(3540..4085)
                     /gene="pilO"
                     /locus_tag="PKI05_02955"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOA51186.1"
                     /translation="MSTPMVVLGKRNMVLVAVVAAITIALIVANMALGYRNTMLRQSI
                     GALRSRIQGQEVQLLAEKASDPQATEIEDFLTDWERRQIDVKSISEFTTQLAHEATSA
                     GLQVVRITPDKWSTTGWLSSCPFNLEFRGTFQQLLRFLRHWDYVGKAVVIHGVDIERA
                     AETGNSLQIRIKFTVYGEKLG"
     gene            complement(4082..4855)
                     /locus_tag="PKI05_02960"
     CDS             complement(4082..4855)
                     /locus_tag="PKI05_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51187.1"
                     /translation="MSIQFDFLPKEYHQTNLRRRNVPWLVSMVLLVVLVGSGGLVVQM
                     GTRRQLWTTYQQLTQQCMANEVKLAQYQHQKAKKRDALRKARLLQPILLGYPRSWLLE
                     AIVSSTPEDVVLDQVRLTLKEEQQVTESQEAPVDFLRSGSGGSADKATEELIRAQEKL
                     SRAWIEVVIDGRSVSTTAVQDFCSQLSHFPVIEEINLQTVEGMSNEKIGQGSTHFVLW
                     GRCRHLPPPAAFVRPDQVPAMTTGPLANVGRHGSKANLP"
     gene            complement(4927..>5457)
                     /gene="pilM"
                     /locus_tag="PKI05_02965"
     CDS             complement(4927..>5457)
                     /gene="pilM"
                     /locus_tag="PKI05_02965"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOA51188.1"
                     /translation="RRQSDRTQTMLIIRMGAQHTLVMILRGDQPLFAKYLNFGGHDLD
                     QALVKQFQIPIESALVLRQGYGERRRDHRDPEVVRTVEECLEPLWNSWLEEIARCARY
                     YQVTFRGQPIERVILAGSEATPELREKLESFLDLPTEVLDPFRAIRCHETVDAARDWD
                     LAIGLALYSPATPCLV"
BASE COUNT         1223 a         1534 c         1380 g         1320 t
ORIGIN      
        1 tgagtctttc cagactaggt agacccaact gagactgccc ttctttttgt tacaaccaat
       61 tccttcggtg tcaccagcag ttcggataca ctcgatacat ccggcacgct ccacctacta
      121 ctgacctcca aaggagctat agcgactccg agcttcgctt aacttggccg ccacttcgtt
      181 ctcgatggtc tgccttcgat agcgtcgcat atccgagaga gactgccgat gaacatcagg
      241 tgtatctatt ggagcacatt gagcctcagc atgagttggc catggatgcg gctggactga
      301 gcgacatcct agggaacaaa gaagccccaa aaccgagaga ctaaaaccga tcaccagcaa
      361 acgacgcttg cccgccaaca tttgttgcat cagatcaact tagagcatga cgcttgttat
      421 actaccgggc actttctcgg aaatgtcaag aaactcttca caataacaga caaaaacctg
      481 cggcagaatt atcagcggtt tgacggtcgt aaagtcgcgc tagtattttg cgttgccaca
      541 ccagctagct gatcgcgaaa ggcaatagct tccaggttcg acggatcaaa gtgcacagcc
      601 atttcggccc agtatttcgc agcgtcgcaa cgtccctgga gatacgccgt ttttgctttc
      661 tccaggaaat gctttgaggc attttgctta tttagtccac tcatctcctc tcccgccacc
      721 tcttggcgtc gttgccatga cgcttgggcc gtggcaccgt ctgtagccat tcgataatca
      781 tcgataatgt agggcgtgat caagacaatg atttcttgct ttctcgttgt ttccatcttg
      841 ttccggaacg ccgcaccgat gagaggtaga tttccaaaaa aggggatttg cgaggccgtc
      901 ttttccaagt cttctcgcat caaaccaccg atgacgaccg tcacgccgtt ggggaccatg
      961 atgttcgtcg tgacctgcgt cgtgtcctta ttaggaaccg taaagttgtt cgtaatctgc
     1021 acggagcccg tggagagttc cgggtgaacc tccatccgaa tcaggccatc gttggaaatg
     1081 aacggccgaa tcacaagctg ggctcccacc ttcaaaaaat ccacggtctg cgaggaactg
     1141 gtctcggtaa acgttgtgct cacgtaccca agttcttttc ctattagaat ttccgctctg
     1201 tgtttgttga gaacgagaag ccggggcgta gcgatgacgt tggtatcgcc gatcgactct
     1261 aaggcgttga ggaaggcacc gatgctcccg tcgagaaagg ccaacttaag accattattg
     1321 tcaaacttga tatcgcttag actttggggg acaagtcccc aaccgagctt gatgtatcct
     1381 ttatttcgca atagctcaaa gctcacccca aactggtcct tgtcgtcaag attgacgctg
     1441 agaatcatgg cctcgatggc gacctgcgcc ggccggacat caacctcctg aacaacgcgg
     1501 tcaacctcct tgagaacccg ctgataatct cggacaatga gcacgtccgt tcccgcgtat
     1561 ctgtcacccc cagcattgct tgggtcgggc gcaatcccgt tctctgccgc tgtggaaacg
     1621 gtaatcagac ctacttgggg tgtgagaaga ggctttatca gcgactctaa ctcctttgcc
     1681 ggcgtgtagt tcgggcgata aatgcgggtg gctatctggt cggtgatgta gcccaggttt
     1741 tgaatctctt gttccgtccc gatataaagg acaccctcat gctcttgtga tacgaacccc
     1801 gggaccctaa gaacggcaaa aatggcgtcg cgcggggtca gagtgttgag agagacggtg
     1861 accttacctt ggacgctgct cgacatgacg acattgagct tggccagctt ggcaagggtg
     1921 tggacggcat cccggatgtc ggcgtcacgt agttcgagtt ggattcccgc ctgattgacg
     1981 gtaatctccg cttgaggcgg attgatcgtc atctctctta tatcgggcaa tgggctgggg
     2041 acaacttcct gggttgagcg ctcttgagaa ggtccgctac gcccctgcgc gtcagattgc
     2101 aatccggaga gagcttcgct atttccgccc ggcgggacct gaagcgtcgg gtcgtccacg
     2161 ttggataagc ccctctcgga tactgcactt gggttcgctt cttggtacgt aattggtgca
     2221 acgcggcctc gatccggtga ctcttcaagc gatgtcacac gaagccgaac gggactgccg
     2281 acatcggcta ccggcgaact gggatccacc tcgtaattga ccggcacgat ggttcgcgat
     2341 tctgtctctt gcggggaaac cataacggaa tccggctcag accgtgccgg caagtcgtaa
     2401 cctgttgtga ctaaccgatc tggaaaagct gtagccgttg agggggaatt gcttataaaa
     2461 ggattgacta cagtaccggc gacggctttg tttggcttgt cttcggactg caccttattc
     2521 tgaatataaa cttggggacc aggctcatac gagggtaatt gctccacatt ggatggccga
     2581 gagtatgaat ttgcctcacg attaggggaa gtttgtaagg ccgtctctgc gggagatgtc
     2641 gatgccttcg tatgggtcac cagctcagca ccgggcccaa catccgacat ggcaagccaa
     2701 taggctgcca ctgtagccag gccacaagcg gcgataatct ccaaaaacct gcgtagccgt
     2761 ttcatcggtc gtccgtgaca gttactcatc catgtcttta gctatgaggt tgccgcttat
     2821 ctaaggtgcc tcgctgccct tggggctcag cccaagctcg ggcagcgatt gttcgaaaac
     2881 agccccgtcg atggtaagtt tgatagccgt cggcgtaact tcacaaatcc ggacaacata
     2941 agaccgtcca tttcggtgga ccttaaatgc ggagccttcc caaagcaagc ggttttcgag
     3001 gatcgctgcc cgccagtcac ccgcgataac cacgccggta aagcgaagtc ccagcgtcgc
     3061 cggagaaacg tcgtcaacat cagatttgcc caacgactct tctgctgact ggtgtacagg
     3121 tgggctcttc atgacaaaag ggtcccgcgc gagctgcgcc gaagcagtct cggcttggaa
     3181 aggctgagca cgccatgcct ggagtacatg ccaaggcgtt aagtccactt ttgcttcctc
     3241 gctactcgcc actatggatc cttcttgcgt gggaaagacc gggccatcgg tttccgtttc
     3301 cgcctgtccc attccgctca ccgaagcctg ttgggaaacc gagcgaggag ccgcggccaa
     3361 ccgatacccc caaaaagcca cggcaaccac cgccaaacct cccaaaacag cggccttgcc
     3421 tggacttcgc ttaaactctc gttttatctt ttttatccat ttgctcatgc gtcgcgtcac
     3481 ctttgggtgc tgggcgccac atcattcatc ggaaatcctt cggattttcc ctaaactaat
     3541 taacccaatt tttcgccata aacggtaaac ttgatccgga tctgcaaact gtttcctgtt
     3601 tcggcggctc gctcgatgtc aactccatga atgaccacgg cctttcccac gtagtcccag
     3661 tgtctgagaa atctcagcag ctgttggaaa gtgcccctaa attccagatt gaacgggcaa
     3721 gaactaagcc acccagtggt tgaccattta tctggcgtga ttctgacgac ttgcaacccc
     3781 gccgaagtgg cttcgtgcgc cagttgcgtg gtgaactctg agatggactt gacgtcaatc
     3841 tgacggcgct cccagtccgt taggaagtct tcaatctccg tggcctgggg gtcggaggct
     3901 ttctccgcca acaattggac ctcctgcccc tggatacggc tacgcaatgc accgatcgac
     3961 tgtcgtaaca tcgtgttccg gtaaccgagg gccatgttgg cgacaataag cgcgattgtg
     4021 attgccgcca ccacggcaac cagcaccata tttctttttc cgagaactac cattggggta
     4081 ctcatggtaa gttcgcctta gatccatgcc ggcctacgtt tgccagcggc cccgtggtca
     4141 tcgctggaac ctgatcgggc cgaacaaaag cggcaggcgg cgggagatgc cgacatcggc
     4201 cccacagaac gaagtgggtt gatccttgcc ctatcttttc gtttgacatc ccttccaccg
     4261 tttgcaagtt gatttcttcg atcacgggaa aatgagagag ttggctgcaa aaatcttgca
     4321 ccgccgtggt cgacacactt cgaccatcaa tcacaacctc gatccaagcc cgcgacagtt
     4381 tttcttgagc gcggataagc tcttcagtag ccttatccgc agaaccgcct gaaccggagc
     4441 gcaagaagtc aaccggcgcc tcttgagact cggtaacttg ctgctcctcc ttaagagtca
     4501 atcttacttg gtcaaggaca acgtcttccg gtgtgcttga gacgattgct tccagtaacc
     4561 acgaccttgg gtaacctagc aagatgggct gcaaaagccg ggccttccga agggcgtctc
     4621 tctttttggc tttttggtgt tggtattgag ccaacttcac ctcgtttgcc atacactgtt
     4681 gagtcaattg ctggtatgta gtccacaatt gacgacgcgt tcccatttga acgactagcc
     4741 ccccgctgcc aacgagcact accaaaagaa ccattgacac cagccagggg acgttccgcc
     4801 gcctgagatt ggtttgatgg tattctttcg gtaagaaatc aaattggatc gacatcgttc
     4861 aaacaactta actcattcta agttggcccc gctcttacga accgcttttc ctttcggctg
     4921 tatcctctaa accaagcacg gagtcgcggg agagtaaagc gccaagccaa tggccaggtc
     4981 ccaatcccgt gccgcatcga ccgtttcgtg acatcgaatc gcacgaaacg gatcgagcac
     5041 ctccgtgggc aagtcaagaa aactttcaag cttttctcgc agttccggtg tggcctctga
     5101 gccagctagg atcacacgct caataggctg gccacggaac gtcacctggt aataccgggc
     5161 acaccttgca atctcctcaa gccaagagtt ccacaatggc tcgagacact cctcaaccgt
     5221 tcgtactacc tctggatcgc gatggtctcg tcgtcgttcc ccatatcctt ggcgaaggac
     5281 caaagccgac tcgattggaa tttggaattg cttgaccaag gcttggtcca gatcgtgccc
     5341 cccaaagttc agatactttg caaacagtgg ttggtcacct ctcaaaatca ttacaagcgt
     5401 gtgttgggcc cccattcgga taatgagcat tgtttgggtt cgatcacttt gccgccg
//
LOCUS       DAOPTR010000096         5431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_12258_length_5431_cov_3.143601, whole genome shotgun sequence.
ACCESSION   DAOPTR010000096 DAOPTR010000000
VERSION     DAOPTR010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12258_length_5431_cov_3.143601"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            316..1029
                     /locus_tag="PKI05_02970"
     CDS             316..1029
                     /locus_tag="PKI05_02970"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOA51189.1"
                     /translation="MAPSLTEKCRELAVSAMKDGISQAALEVLKQYGYEGLTMDRVAE
                     VAGIAKGSLYHYFRNKQELVTYLFDQTIEPAIQAGEEIFQRVVPAVEKLEAMVRMWFD
                     YFSQHRALFDFLFRDPAVRELCLTSRRSKNGLAIDRFSAILRQGISEGVFRPLDVTYA
                     AEMIIGSLQFVIERQLETGEAHPIEVSVRRLMDIVLHGIGASSASAESPSAIGQRCEA
                     PQVEGKTGESSEPSQVAAE"
     gene            1194..2438
                     /locus_tag="PKI05_02975"
     CDS             1194..2438
                     /locus_tag="PKI05_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOA51190.1"
                     /translation="MNRKKLLQGIISLGFAALVVGVGIALAMLHSWMFGDEKTPSATQ
                     TSAVSSGAKVGDRDAVPVVVQAARTMVFEHRLDVSGNVLAKRFALVSARIPGTLDAIF
                     VDEGDFVEAGKTKLFQTDSVKLTQAVAIAQEQVTVAECAVREKEALLEKTLVAQQVAE
                     ADLARYKDLAQRNAIARQVVEHQEAQVRQLQADVRHVQTLIELSKAQLEQARLNLKIA
                     EKDLGDSLVVAPISGRVSLRMKEPGEMAGAGTPVLRIDDLSLVEVSIFVPSEYYDQVV
                     VGETKLRARVAGRDLGELPVTFKSPVIESRLRTFQVKALVQAPPPEVVPGALAQVSLI
                     LGRRQGLGVPKQAVQQRSGKNIVFIVEDNKAKAVAVELGWETEGWQEIRAGMSAVAPV
                     ITSGQSLVDDGTPVRVLEEAGR"
     gene            2435..>5431
                     /locus_tag="PKI05_02980"
     CDS             2435..>5431
                     /locus_tag="PKI05_02980"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOA51191.1"
                     /translation="MKIADFAVRRPVATSCLIIGLTLLGLNAFRKMGLELMPKIDIPY
                     ITVVTIYPGASPEELEIDVAKRIEDAVSSIEGLKHVSSICMENVCQTELEFEMEVDVD
                     IAATDVREKIDLIRSELPEDVEDPIIQKFDINAKPVATMALVGDVPLDELYDYADNEL
                     RDRLTVIQGVADVQLIGGAKREVQLRLDREKLAARGLTTMDVVKAVQASVKTIPAGRI
                     REAGTEYSVKFDADYETVAGLNDLEIANQEGKRCYLRDVGEAVLATEEVRQLATLDDK
                     QCIAIKVIKKADANPVAVANAVKQALERINRELPSGMQLVWVTDDATFIEAMNLDAWK
                     NVAIGIGLTAAILFFFLYELRSLIIVSITMPLTIIIGLFFMQAVGYTLNTPTLIAIGL
                     SVGILVTNSIVVLESIIKRLEELGDPKEAARIGANEVFVAVLASAITNVVVLFPLAVM
                     KTRVGLFIAPLAMTMFILTLVSLFISFTLTPMLCSLILKPQKRSRSLLRRIGEGFNRF
                     LEANVSAYRWLLGFTEHYRVVGALVLLAVVGMFVHSLWVAGRLGTGFFTETDRGEVYV
                     RLEFPTYYDLAATTAKINEAKRRLEGLPELKHMLTTIGKAEAVLGQSSEGVYLGQMLL
                     VFSRRTERKLTIYQLMDIVEQRLQDFPDARIVVSQASLMGGQSNPVEMEIAGPDLQKL
                     DQLALASLRLAREIPGVLSPDTSVREPKPEIRVRPRRPILADINSPAVGMGLTLRGNL
                     EGIKAGTFKSRETARNYDIVVKFMEQEGKQQVAGFMFPGVEGHALILATLGTIEERKI
                     PIQIVRKDKQRISKVTAQLAPDLPLGKAVTLISERVNREVQLPPGYRYSFGGIYEVMQ
                     EGLGGLFEAFIISVLLVFLSLAAIIESFKRTFLVVVTVPMALIGTFWALAIAGQSLDI
                     FAVMGIVMMTGIVVNNAILIIDQFNVLIAQGVPRHQAMIQAACDRFRPIVMITLAAVL
                     GMLPLALGRGIGAEPRVGVGVATVGGI"
BASE COUNT         1137 a         1352 c         1695 g         1247 t
ORIGIN      
        1 ggctcccagg ccaagatcaa ttttcccgaa aacggtaccc gaaaaatgcc aaaagtcgag
       61 attagagtcc atcccaaaaa gtccccctct ccttccggga gagggttggg gtgagggtga
      121 gcgatcgtcg gagttgtttg agaaaaccag cagcgagtgg agtcatccgc acgggccatc
      181 cctctcccgc cgaacggccg aggggaggtt atgggaaagg ttcttaaatg acacgccgaa
      241 tccccgacgg tggttaaccg ctgattgagc ggagctaaca acagaggtgt tgtcagtaaa
      301 aggtagggac aagttatggc tcccagtttg acagaaaaat gccgcgagct tgccgtttcg
      361 gccatgaaag atggcatcag tcaggctgcc ctggaagtcc tcaaacagta cggatatgag
      421 ggcttgacca tggaccgggt ggcagaggtg gccgggatcg ccaaggggag tctttatcac
      481 tacttccgca ataagcaaga gttggtgacc tatcttttcg accagacgat cgagccagcc
      541 atccaagcgg gggaagagat tttccagcgg gtggtcccgg ctgtggaaaa gcttgaggcc
      601 atggtccgga tgtggtttga ctatttttca cagcaccggg cactttttga tttccttttt
      661 cgtgatcctg cggtccgcga gctttgtttg acctcccggc ggtccaagaa cgggctggcc
      721 attgatcgct ttagtgcgat tttgcggcag gggatcagcg aaggagtgtt tcgtcccctg
      781 gatgtaacct acgcggcgga gatgattatt ggctctttgc aatttgtcat cgagcgacaa
      841 cttgaaacgg gtgaggccca tcccattgag gtctcagtgc gacgcctgat ggatattgtg
      901 cttcacggga tcggtgcctc ttcggctagc gcggagagtc cgtctgcaat aggccagcga
      961 tgtgaagcac ctcaggttga ggggaagact ggggaaagca gcgagccttc tcaagtggct
     1021 gccgagtaac cgcggggcca aacgcagaga agcaggagaa ttgcgaggag gcaatcacaa
     1081 ggtgtgccgc aatttttgtt gggggcgggg tgtgaggaaa cgccttgtgg gggaaatttg
     1141 agggtcggga tgtgccaagt tggacttcaa gagcgtgtta aggggaatac gccatgaatc
     1201 ggaagaaact gctccagggg atcatcagtt tggggttcgc cgcccttgtg gtgggcgtcg
     1261 ggatcgcatt ggcgatgctt catagctgga tgtttggtga tgaaaagacc ccatccgcta
     1321 cccagacttc cgcggtgtct tcaggggcaa aagtggggga ccgagatgcg gtgccggtgg
     1381 tcgttcaagc ggcacggacg atggtcttcg agcatcggct tgacgtctcg gggaacgtct
     1441 tggctaagcg atttgccctg gtctcggcac ggattcccgg aacgttggat gccatctttg
     1501 ttgatgaagg ggactttgta gaggcgggca agacgaagtt gtttcagacg gattccgtga
     1561 aattgaccca ggcggtcgcc atcgcccagg aacaggtaac cgtggcggaa tgtgcggtcc
     1621 gcgagaaaga ggccctcctg gaaaagacgc tcgttgccca acaggtggcc gaggccgatt
     1681 tggctcgtta taaagacctt gcccaacgaa acgcgattgc gcggcaggtt gtggagcatc
     1741 aagaggcaca ggtgcgtcaa ctgcaagccg atgtccggca tgtccaaaca cttattgagc
     1801 tgagcaaggc gcagcttgag caggcgcgct tgaatctgaa aatcgccgaa aaggacttag
     1861 gggattcact cgtcgtggca ccgatttcgg gtcgcgtctc gctccggatg aaggaaccag
     1921 gcgaaatggc tggagcgggc acgcccgttt tacgcattga tgacctttcc cttgttgaag
     1981 tgagtatttt tgtcccgtcc gagtattacg atcaggtggt tgtgggagag acgaaactgc
     2041 gggcgcgggt ggcgggacgt gatttgggcg agttgcctgt cacgtttaag agtcctgtga
     2101 tcgaatctcg gttgcggaca tttcaggtga aagccctcgt gcaagctcct ccgccagagg
     2161 ttgtccccgg tgctttagct caagtgagcc taatcctggg cagacgccaa gggttgggcg
     2221 taccaaagca ggctgtgcag caacgttcgg gcaagaacat tgtgttcatt gtggaagaca
     2281 ataaggccaa ggccgttgcg gtggaactgg gatgggagac ggagggctgg caggagattc
     2341 gtgctgggat gtctgctgtg gcgcccgtaa ttaccagtgg ccaatcgctg gttgacgatg
     2401 ggacgccggt tcgtgtcttg gaggaggcag gccgatgaag attgccgact ttgctgttcg
     2461 ccgtcccgtt gccacgagct gtctcatcat tgggttaacg ctcctgggcc tgaatgcctt
     2521 tcgcaagatg ggcttggagc tgatgcccaa gattgatatt ccctacatca ctgtggtgac
     2581 gatttatccg ggtgcctcac cggaggaatt ggaaatcgac gtagccaagc ggattgagga
     2641 tgccgtctcc tccattgagg gtctcaaaca cgtttcctcc atctgtatgg aaaatgtgtg
     2701 ccagaccgag ctggaattcg aaatggaggt ggatgtcgat attgcggcca cggacgtgcg
     2761 tgagaagatt gacctcatcc ggtcggaatt gccggaggat gtcgaagacc ccatcattca
     2821 gaagtttgac atcaacgcca agccggtggc gacgatggcc ctggtgggcg acgttccgct
     2881 cgatgaactt tacgattacg ccgacaacga gcttcgcgat cgcttgacgg tgattcaggg
     2941 agtggcggat gtccagctca tcggtggcgc caagcgggaa gttcaactgc gattggaccg
     3001 cgaaaaactg gcggcgcggg ggctgaccac catggatgtc gtcaaggccg tgcaggctag
     3061 tgtgaagact attcccgcgg gccgaatccg cgaagcagga acggagtatt cggtcaagtt
     3121 cgatgccgat tatgaaacgg tggcgggcct caatgacctg gagatcgcca atcaggaagg
     3181 gaaacgttgt tatcttcgtg atgtgggtga ggcggtactg gcaaccgagg aggtccggca
     3241 gcttgctacc ctcgacgaca aacagtgcat tgccattaag gtcatcaaaa aggccgacgc
     3301 caaccccgtc gcggtggcca atgccgtgaa acaggctctt gagcgaatca accgcgagtt
     3361 gccgtcgggc atgcagcttg tttgggtgac tgatgacgcc acgtttattg aagccatgaa
     3421 cttggacgct tggaaaaacg tggccatcgg tattggcctg acagcggcga ttctcttttt
     3481 cttcctgtat gaacttcgct ccctcattat cgtctcgatc acgatgccgc tgacgattat
     3541 tatcggtctc ttcttcatgc aggccgtggg atataccctc aacacaccta cgttaattgc
     3601 catcgggttg tcggtcggta ttttggtcac caatagcatc gtggtgctgg agtcgatcat
     3661 caagcgactt gaagagttgg gagacccgaa agaggcggcc cgaatcggag ccaatgaagt
     3721 gttcgtggcc gttcttgcca gtgccatcac caatgtggtg gtgctctttc ctctggcggt
     3781 gatgaagacc cgcgtgggac ttttcatcgc acctttggcg atgacgatgt ttatcctgac
     3841 gctggtgtcg ctgtttattt cgttcacgct gacaccgatg ctgtgctcgc tgatcctcaa
     3901 gccgcaaaaa cggagtcgtt cgcttctccg tcggatcggt gaaggattca atcggttcct
     3961 tgaggccaat gtctcggcgt accggtggct tttgggattt actgagcatt atcgggtggt
     4021 gggtgctctg gtcttgcttg ccgttgtggg gatgtttgtc cattcccttt gggtggcagg
     4081 ccgattgggg acgggtttct ttacggaaac cgaccgcggc gaggtttacg ttcgcttgga
     4141 attccccacc tattacgatt tggccgccac cactgccaag atcaacgagg ccaaacggcg
     4201 tcttgagggc ctaccggaac tcaagcacat gctcaccacc atcggtaagg cagaggccgt
     4261 tctcggtcag tccagcgaag gcgtttacct cggccaaatg ctgctggtct tttctcggcg
     4321 gacagagcgc aaattgacca tttatcagtt gatggacatt gtcgagcaac ggcttcaaga
     4381 ctttcctgat gcccgcattg tggttagcca ggcgtcgctg atgggtggtc aatccaaccc
     4441 cgtggaaatg gagattgcag gtccggacct tcaaaagctg gaccagcttg cgttagcgag
     4501 tttgcggttg gcccgggaga ttccgggggt cttatcgccg gatacctcgg tccgcgagcc
     4561 taagcccgag attcgggtgc gaccacgccg cccgattttg gccgacatca actcgccagc
     4621 ggtcggcatg ggactgacgt tgcgcgggaa cctcgaaggc atcaaggcgg ggaccttcaa
     4681 atcgcgtgag acggcacgca actacgatat tgtcgtcaaa ttcatggaac aagagggtaa
     4741 acaacaggta gccggtttca tgttcccggg ggttgagggt catgccctga tcctcgccac
     4801 gctgggtacc atcgaggaac gaaagattcc catccagatc gtccgcaaag ataaacagcg
     4861 tatttccaaa gtgacagcac aacttgctcc cgacttaccc ttgggtaagg cagtcacgct
     4921 gatcagcgag cgggtgaatc gtgaggtgca gcttccccct ggctatcgtt acagcttcgg
     4981 agggatctac gaggtcatgc aagagggtct tgggggccta tttgaggcct ttatcatctc
     5041 tgtgctcctg gtcttcctgt cactggcggc gatcatcgaa tccttcaagc ggaccttcct
     5101 ggtcgttgtg accgtaccca tggcgctgat cgggacattc tgggcattgg ccattgccgg
     5161 gcaaagcttg gacatctttg ccgtgatggg catcgtcatg atgacgggaa tcgtcgtcaa
     5221 caatgccatt ttgatcattg accagttcaa cgtcctgatt gcccaaggag tgccgcggca
     5281 tcaggcgatg attcaggctg cctgcgaccg gttccggcca atcgttatga tcaccctggc
     5341 ggccgtattg ggtatgctgc cccttgccct aggtcgcgga attggggcgg agccacgggt
     5401 cggcgtcggt gtggccacgg tgggtggcat t
//
LOCUS       DAOPTR010000097         5424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_12274_length_5424_cov_3.704600, whole genome shotgun sequence.
ACCESSION   DAOPTR010000097 DAOPTR010000000
VERSION     DAOPTR010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12274_length_5424_cov_3.704600"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..1278
                     /gene="tuf"
                     /locus_tag="PKI05_02985"
     CDS             79..1278
                     /gene="tuf"
                     /locus_tag="PKI05_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOA51192.1"
                     /translation="MAKDVFVRTKPHVNVGTIGHIDHGKTTLTGAILAVQSKKGLAKF
                     KSYADIAKGGTVRDATKTVTIHVAHVEYETEKRHYAHIDCPGHADFIKNMITGAAQMD
                     GAILVVSAADGPMPQTREHVLLARQVNVPAIVVFLNKIDLVDDPELLELVEMELRDLL
                     KKYDFPGDEVPIIRGDALSAYQNPDDPEKTKCIQELLDAIDNYIPEPVRPIDKPFLMA
                     VEDVFSIEGRGTVATGRIEQGTIKPGDEVEIVGFSRESRKTVVTSIEMFNKTLDEGRA
                     GDNVGCLLRGIKKDEIERGQVLAKPGSITPHTKFEAEVYILSKEEGGRHTPIMSGYRP
                     QFYFRTTDVTGEIQLTGGAEMVLPGENVKIQVELISPVAMQENLRFAIREGGRTVGSG
                     VVTKILE"
     gene            1426..1499
                     /locus_tag="PKI05_02990"
     tRNA            1426..1499
                     /locus_tag="PKI05_02990"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1460..1462,aa:Trp,seq:cca)
     gene            1555..2016
                     /gene="secE"
                     /locus_tag="PKI05_02995"
     CDS             1555..2016
                     /gene="secE"
                     /locus_tag="PKI05_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOA51193.1"
                     /translation="MAAIVMAQAASAKFNSVFGRFLTGLVRADLYKRNQGRVVRQATF
                     GAIVAFGLFGAWRLYGTLGSWGFEASWQFGVPAAVFAIAVWLAYRLMNFPEVADFLIA
                     VEAEMRKVTWPTRAELIRSCVVVLVTMFSLAFCLTIYDFIWASFLRWTGIL"
     gene            2170..2967
                     /gene="nusG"
                     /locus_tag="PKI05_03000"
     CDS             2170..2967
                     /gene="nusG"
                     /locus_tag="PKI05_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOA51194.1"
                     /translation="MSDELEQRQDAVQEDGGVAGSPEGFSPEQSAASSLGAESANGAE
                     STGAAEMEPPVERKEEAKKEKRPRKKKEAEKPQEACGAAGDTSRMGWYIVKVQTNRED
                     SACEALSRRIKVAGMDQYFGQIIVPVERVTEVKGGKKRVVRRKLYPGYIVVQMELNDD
                     TWFLVRETPGIGDFTGAVGRPTPLLPHEVNRILAKLAEERAEDAPKLKIGCRVGDKVK
                     INEGHFANFQGEVSAIDEANGRVTVMINIFGRSTPVELEYWQIEPAE"
     gene            3039..3464
                     /gene="rplK"
                     /locus_tag="PKI05_03005"
     CDS             3039..3464
                     /gene="rplK"
                     /locus_tag="PKI05_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOA51195.1"
                     /translation="MAKQLVGTVRFCVPGGQATPAPPVGTSLGRFGINLGQFVQQFNE
                     RTRQHLGLRIPVVVRVYNDRSFELETHTPYTADLLKQAAGIAKGAGNPPKEKVGRVTR
                     SQVMEIAKVKMPDLNARDIEHACRIIEGTARSMGLDVVD"
     gene            3613..4293
                     /gene="rplA"
                     /locus_tag="PKI05_03010"
     CDS             3613..4293
                     /gene="rplA"
                     /locus_tag="PKI05_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOA51196.1"
                     /translation="MGKRSKRYRSLASKVPSKGPLSLEEAVKLLKTFGNTKFDQTVEI
                     AIRTGIDARQADQTIRGSVVLPHGLGKPVRVVVFAKGDRADEAKAAGADEVGGPELAE
                     RIKGGWLDFDVCIAAPDMMGVVGPLGKILGPRGLMPSPRAGTVTPDVGKAVREYKAGK
                     VEFRNDSGGNIHAPVGKLSFDEQKLVENIKAFIEHVQTLRPASLKGQFIKNVSLSATM
                     SPGIRVAI"
     gene            4388..4942
                     /gene="rplJ"
                     /locus_tag="PKI05_03015"
     CDS             4388..4942
                     /gene="rplJ"
                     /locus_tag="PKI05_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOA51197.1"
                     /translation="MSKYVKNLITEHYRRRLAGVDAALLVNVVGLDANRTRALRKDLA
                     EKNIHLLVVRNSLVRRALQGTPLAKAFENVTGPTAICWGGEDIVALSKEVMKYASDEK
                     YAPFAAKGGVLDGEALTAEQVKEISKWPSRQELLAQLAGRLLGPAGALMSALAGPGTT
                     LAGQLKQLAEGEKGDAPAAGESGG"
     gene            5074..>5424
                     /locus_tag="PKI05_03020"
     CDS             5074..>5424
                     /locus_tag="PKI05_03020"
                     /inference="COORDINATES: protein motif:HMM:NF027644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bL12 family ribosomal protein"
                     /protein_id="HOA51198.1"
                     /translation="MATDAPVREFSQRVKELGDAIVALPLKEVKELNDYLEIVHGIKP
                     AAGAVMVAGGPAAASGAGAAAAPQQEKTEFDVILENFGANKLKVITAVRTLIPGISLA
                     DAKKLVEGAPSKIKE"
BASE COUNT         1234 a         1252 c         1784 g         1154 t
ORIGIN      
        1 gagccggccg gtaaatcgtg tcaagtgacg atattgggtt agttcctgtt agaaccagag
       61 aacggagatt cagcaagaat ggctaaggac gtatttgttc gaacgaagcc acacgtgaat
      121 gtggggacaa tcggtcacat cgaccatggg aagacaacgc tgaccggtgc gattttggcg
      181 gtccagtcga aaaagggatt ggctaaattc aagagctatg ccgacatcgc caaaggtggg
      241 acggtccgcg atgccacgaa gacggtgacg attcacgtcg ctcacgtgga gtatgaaacg
      301 gaaaaacgtc actatgctca catcgactgc cccggtcacg ctgacttcat taagaacatg
      361 atcaccggcg cggcccagat ggacggggcc atccttgtgg tgtcggcagc ggatggcccg
      421 atgcctcaaa cacgcgaaca cgtgctcctt gcccggcagg tgaatgtccc cgcgatcgtg
      481 gtcttcctca acaaaatcga cctcgtggac gatccagagt tgttggaact ggtcgagatg
      541 gaacttcggg atttgctcaa gaagtatgat tttccgggcg acgaggttcc gattatccgc
      601 ggcgatgctt tgtccgccta tcagaacccg gacgatccgg aaaagacgaa gtgcattcag
      661 gagcttttgg acgcgattga caactatatt cccgagcctg ttcggccgat tgacaagccg
      721 ttccttatgg ctgttgaaga cgtcttctcg attgaaggcc gtggaacggt ggcgactggt
      781 cgtatcgagc aggggactat caagcccggc gacgaagtcg agatcgtcgg attttctcgc
      841 gaatctagga agaccgttgt gaccagcatc gaaatgttta acaagaccct cgacgaggga
      901 cgggctggcg ataacgttgg ctgcctactt cgtggaatca agaaggacga gattgagcgt
      961 ggccaggttt tggccaagcc gggcagtatc acgccgcata cgaaattcga ggccgaggtc
     1021 tatatcctgt ccaaggaaga aggtggccgt cataccccca ttatgagtgg ttaccggccc
     1081 cagttttatt tccggaccac ggacgtgacc ggtgagatcc agttgaccgg cggtgccgag
     1141 atggtcctcc ccggcgaaaa cgtgaagatc caggtggagt tgatttcgcc cgtggcgatg
     1201 caagagaacc ttcgtttcgc tattcgcgag ggtggccgaa cggtcggctc cggtgtcgtg
     1261 acgaagattc tggagtaaaa gatttaatca gaacggcaca aacggcgcat aacgacgcgc
     1321 cccccggttg gcatggctgt ccatgttcgg ggaccttgtt atgcgtcggt cggaggtgta
     1381 ttctgagata atggagtgcg gcccgtttgc gattgggctg caaacagggg tgtagctcaa
     1441 tttggtagag caccggtctc caaaaccggc ggttgggagt tcgagtctct ccgcccctgc
     1501 ctttccggat gatggttgcg cctattgagc gcatgttcgt tttcgaattt ctagatggcg
     1561 gcgattgtga tggctcaggc cgcaagtgct aagttcaata gtgtcttcgg cagattttta
     1621 actggactgg ttcgcgctga tctatacaag cgtaaccagg ggcgcgtggt ccgtcaggcg
     1681 acgtttggcg ccattgtcgc gtttgggtta tttggggcct ggcggcttta tgggacatta
     1741 ggctcctggg gttttgaggc ctcatggcag tttggtgtgc cggccgcggt gtttgcgatt
     1801 gcggtttggc tagcgtaccg gctgatgaat ttccccgaag tggcggattt tctcatcgcc
     1861 gtcgaggcgg agatgaggaa ggtaacttgg ccaacccgtg cagaattgat aagaagttgt
     1921 gtagtcgtcc tcgtgacgat gtttagcctg gctttttgcc taactattta cgacttcata
     1981 tgggcctcct tcctccggtg gacgggaatt ctgtgagctt gcctttgggt tggggggtgg
     2041 gtaagtcttt ctggcggttt tgtgcaatgt agttttcgcg acgtccgcgg taccaagacc
     2101 cgctagccat acgattgagg tggatgtaaa tttgatattt tcggccgctt gagagggtgt
     2161 accccttaca tgtctgatga actcgaacaa agacaggatg ctgtccagga agacggcggg
     2221 gttgcgggca gcccggaggg gttttcgccg gagcaatcgg ccgcttcctc tttaggcgct
     2281 gagtccgcaa atggcgccga gtccactggg gctgccgaga tggagccgcc cgtcgagagg
     2341 aaagaggaag ccaaaaagga gaagcggcct cggaagaaga aggaagcaga aaagccgcaa
     2401 gaggcctgtg gggcagccgg cgatacgagt cggatggggt ggtacatcgt gaaggtccaa
     2461 acaaaccgcg aggatagtgc ctgcgaggca ctctcgcggc ggattaaggt cgcgggaatg
     2521 gaccagtatt tcgggcaaat cattgtgccc gtggagcgag tcacagaggt caagggtggt
     2581 aagaagcggg tggttcgccg caagctctat cctggctata tcgtggtcca gatggagcta
     2641 aacgatgata cgtggttcct ggtaagagaa accccaggga ttggggactt cactggggct
     2701 gttgggcgtc ccacgcctct tttgccccat gaggtcaatc gcatcttagc gaagctggcg
     2761 gaagagaggg ctgaagacgc acccaaatta aaaatcggtt gtcgcgtggg agacaaggtt
     2821 aagatcaacg aggggcattt tgcgaatttc cagggggaag tttcggcgat cgacgaggcc
     2881 aatggccggg tcaccgtgat gatcaatatc ttcgggcgaa gtaccccggt ggaactggaa
     2941 tactggcaaa ttgagcccgc ggagtgagga tggggaaggg gcagattcgt gcgaatgcga
     3001 ggacagtgat ttcgaataaa aacgaaggtt aggatgcaat ggcgaaacaa ctggtcggaa
     3061 cagtaaggtt ttgcgtgcct ggcggacagg cgacaccggc tcccccggtg ggtacgtcgc
     3121 tgggccgatt cggcatcaac cttggtcaat ttgtacaaca gttcaacgag cggacaaggc
     3181 aacacctggg attgcgaatt ccggtggtag tccgtgtcta taacgatcgg tcgtttgagc
     3241 ttgaaacgca cacgccgtac accgcggatt tattgaaaca agctgcggga attgctaagg
     3301 gtgcgggtaa cccgcctaag gaaaaagtcg gaagggtgac aagaagccag gtgatggaga
     3361 tcgccaaggt caagatgcca gatctgaatg ctcgcgatat cgagcacgcc tgccggatca
     3421 tcgagggcac ggcccgcagc atgggtctgg acgttgttga ctgaggctat tggaaagagc
     3481 gtttcggtaa tgggtggctg acaagtgttt gctgagtaac cgctaaccac ggggacgtgg
     3541 aaatggcgag gtcctgtcgc cgaagagaac gttggccgtg gggtgcggga gaatctgaaa
     3601 aggattcgca aaatgggaaa gcgttcgaag cgttacagaa gtctagcgag taaggtgcct
     3661 agcaagggcc cgctgtcctt ggaagaggct gtcaagctgc taaagacctt cggcaacacg
     3721 aagttcgacc agaccgttga aattgcgatc aggaccggga tcgacgctcg gcaggcggat
     3781 cagacgattc gggggtccgt ggtgttgccg cacgggctag gaaaaccggt gcgggttgtc
     3841 gtatttgcga agggtgaccg tgcggacgag gcaaaggccg ctggggccga tgaagtcggt
     3901 ggcccggaac tggctgagag gatcaagggc gggtggctgg atttcgacgt ttgcattgct
     3961 gcacccgata tgatgggtgt tgttggtcct ttaggaaaga ttctaggtcc tcgtggattg
     4021 atgccctcgc ctcgcgccgg gaccgtaacg cctgatgtgg gaaaggcggt ccgtgaatat
     4081 aaagctggga aggtggagtt tcgcaacgac agtggcggaa atatccacgc cccggttgga
     4141 aaacttagct tcgacgagca gaaacttgtt gaaaacatca aggcctttat cgaacacgtg
     4201 cagacgctca ggccggcgag cctgaaggga cagttcatta agaacgtatc tctatcagcg
     4261 acaatgtcgc caggcattcg agtggcgatc taagcttaag gattcggcgg gagcgaagac
     4321 gtgtttgccc gttgagcgtg gtgcagagtt ccgagcaata agaatataaa tcgacctggt
     4381 acaaaccatg agtaaatacg tcaaaaacct aataacagag cattatcggc ggcggttggc
     4441 gggggtggat gctgcgctgc tggtgaatgt ggtgggcctg gatgccaatc ggacgcgggc
     4501 gctacggaag gacctcgcgg aaaagaacat tcacttgctc gtggtgagga attcgctagt
     4561 tcgccgcgcg ttacagggaa caccgttggc caaggccttc gagaacgtaa ccggtccgac
     4621 ggccatctgc tggggcggcg aagacattgt cgccctttcc aaggaggtga tgaaatacgc
     4681 gagtgacgaa aaatacgcgc cattcgcggc caagggtggt gttctcgacg gtgaggcatt
     4741 gacggctgag caggtcaagg agatcagcaa gtggccgtcg cggcaggagc tgctggcgca
     4801 gttggcaggc cgtttgcttg gcccggcggg tgcgctgatg tcggcattgg ccggtccggg
     4861 gacaacgctg gccgggcaat tgaagcagct cgccgagggc gaaaaaggcg atgcaccagc
     4921 ggcaggggaa tcgggcggtt gatcgatggc tagcgctcga ggcccttcgt gggaaaggct
     4981 gacgcttgtt acagtcctgg aaaacctaga cgtttctgaa ggataccgcg aatttttgta
     5041 gtaggttcga tttggaaagg attggagtca cacatggcga ccgatgcacc agttcgtgag
     5101 tttagtcaga gagtgaagga gttgggggac gcgattgtgg cgctgcccct aaaggaagtg
     5161 aaagagctga atgattacct cgagatcgtc catggtatca agccggcagc aggcgctgtg
     5221 atggttgctg gcggtccggc ggcggcaagt ggggcgggtg cggcggctgc tccgcagcag
     5281 gagaagacgg aattcgacgt tatcctcgaa aactttggcg ccaacaagct taaggtgatc
     5341 accgccgtac ggacgttgat tccgggcatc agcttggccg acgcaaagaa gttggtcgag
     5401 ggtgcacctt ccaaaatcaa ggaa
//
LOCUS       DAOPTR010000098         5421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_12284_length_5421_cov_3.438502, whole genome shotgun sequence.
ACCESSION   DAOPTR010000098 DAOPTR010000000
VERSION     DAOPTR010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12284_length_5421_cov_3.438502"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="PKI05_03025"
     CDS             <1..850
                     /locus_tag="PKI05_03025"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOA51199.1"
                     /translation="QRVITIGPGVTVTMVRIPSGRFVMGNAEGEIDERPPCVVEIPHD
                     FWISTCEITNRQFAVFDPTHDSHVESLHGYQFGYHGFPLNQPDQPVVRVTWHEAVAFC
                     DWLSNRQGVRFRLPTEAEWEYACRAGTAEPFWFGSLQDDFSPYANLADQRLSEFVMDT
                     YIQVRTIANPNHFDDWILKDPRWNDGGLVSVSVGSYRSNPWGLYDMHGNVWEWTLSLY
                     RDYPYADNDGRNDMAASGLRVVRGGSWYDRPKRATSSFRLAYPPYARVFNVGFRIVAE
                     SLPTAE"
     gene            complement(1129..2703)
                     /locus_tag="PKI05_03030"
     CDS             complement(1129..2703)
                     /locus_tag="PKI05_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOA51200.1"
                     /translation="MNFEHKSDFPFCCGCRYPHSKQSVEDRQTTGVTRRDFLGAAAGG
                     VVASAVLATTTHGAWETASHRPPSVKTLQVQPVFNCHIYPRQEATSWRVTGAIQDEAE
                     LREEENRIRADLKKLQEMADFPMEIRPLVTVGTVEEANAVASQPCDVMLVFAARRNVP
                     VLEALIRPERWNLMFVRHRSGPLYYMYIGVHVHYLRKRRDFFAEPAMTIDDVVVDELP
                     ELLWRLRALFAVKNTLGQRIVAIGNPAGWGADGAQAPARAKEVWKFEYITVPYPELEE
                     RLKQARANERLVQHCQQQAEAYLRDAQVSLETERGFVERAFVLTEVFRTLLDEADANA
                     ITVHNCMTTIMPVSRTTACLPLTLLNDDGYLAFCESDLVAIPAGILLHYVTGRPVFFC
                     NPSFPHKGIVTVSHCTAPRRMDGKSLAPARILTHYESDYGAAPKVEMPRGQEVTVIDP
                     DFNGQRWLGFEAAIMDSPFFPICRTQLDLKIIGDWQRLIQEIRGFHWLLAYGRVLKTF
                     GYAVRKAGLDWLPIEA"
     gene            3157..4053
                     /locus_tag="PKI05_03035"
     CDS             3157..4053
                     /locus_tag="PKI05_03035"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOA51201.1"
                     /translation="MFASGTFGNIVDGYPIANLSRLGAIVPKTVTFSPRIGNPPPRTV
                     ETPAGMLNSIGLDNDGLMAFLERRLPALSTIGPPVIVSIAAKDEDEFVKMAEILDAAQ
                     GVSAVELNLSCPNVAGGIDFASDPRKCHVLVARVRQRFSRAILAKLTPNTGDIATIAR
                     AAADAGADAITAVNTLLGLAVDWRRRRSRLGGFFGGLSGPAIKPVALRCVYQIAHAVD
                     IPVIAAGGVTNVDDAMEFFVVGASAIQVGTASFFHPMAAWEIIDALPSALRELGAYSI
                     REIVKTLCEPRKACDLRKTREC"
     gene            4134..5102
                     /gene="trpS"
                     /locus_tag="PKI05_03040"
     CDS             4134..5102
                     /gene="trpS"
                     /locus_tag="PKI05_03040"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011120916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOA51202.1"
                     /translation="MIVVSGIQPTGRFHWGNYFGAIKQYVELQDQADAAYYFIANLHA
                     LTTVRDPQVLRQNTIDAAIDLLALGINPDRATLFIQSDVPEVTQLCWLLMTCTPLGLL
                     ERCHAYKDKRAKGLPADAGLFTYPVLMAADILAYDADTVPVGEDQVQHIEVCRDVASS
                     FNHLYGNVFVMPKAKIMENAARVPGTDGEKMSKSYNNTIGIFDDPKTVRKTIMRIVTD
                     SRPIEQPKAPETDHLFQLYSLFVGEREREEMAARYRRGGFGYGEVKQALADVAEKYFA
                     EAREYRKELESKPQFVREILGDGAAMARKKAAEVLQRAQNACGIKY"
     gene            5242..>5421
                     /locus_tag="PKI05_03045"
     CDS             5242..>5421
                     /locus_tag="PKI05_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOA51203.1"
                     /translation="MRILGIGTDITECLRIARMIDRHGEIFLQRVYTPAEIAYCQNRK
                     QATQHFTGRWAAKEAI"
BASE COUNT         1199 a         1490 c         1509 g         1223 t
ORIGIN      
        1 gcaaagggtc atcacaatcg ggcccggtgt gacggtgacc atggttcgga tcccttccgg
       61 gcggtttgtc atgggcaatg ccgaaggtga gatcgatgaa aggccgccgt gtgttgtgga
      121 gattccccac gacttttgga tcagcacatg cgagatcaca aaccggcagt ttgccgtgtt
      181 cgatccgacg catgatagcc atgtcgaatc ccttcatggt tatcagtttg gttaccacgg
      241 attcccactt aaccagccag atcagcccgt ggtccgcgtc acttggcacg aagctgttgc
      301 cttttgtgat tggctttcta accggcaagg agtacgcttc cggcttccca ccgaagcgga
      361 atgggaatat gcctgtcgag cggggacggc cgagccgttt tggttcggtt cactccaaga
      421 tgatttctcc ccttacgcca acttggcaga ccaaagactg agcgaatttg tcatggatac
      481 gtatattcag gtcaggacaa tcgccaatcc taaccatttt gacgactgga ttctcaaaga
      541 cccccgttgg aatgacggcg gccttgtttc ggtgtcggtc ggctcttatc gtagcaatcc
      601 gtggggactt tacgacatgc acggtaacgt ctgggaatgg accctttccc tttaccgaga
      661 ctatccatat gccgacaacg atggccgcaa tgacatggcg gcatccggac tgcgggtggt
      721 tcgtggaggt tcctggtacg atcgcccaaa gcgtgcaacg tcatccttcc gcttagccta
      781 tccgccttac gcccgggtgt ttaacgtggg attccgcatc gtcgccgaaa gtttgcccac
      841 cgcagaatag taggaaccag ccctaacccg ataaaaagag ctcccaccgc cttgggagcg
      901 tgccgactaa aaaaccacaa tacacgctac agaaagtgtt acgcggttgc ctcccacccc
      961 tgacaggact aagagcctat cccataatct cccctcgccc gttcggcgcg acagcgacct
     1021 agggtgaagt ggctcgaatc gctgccggtt ttctcactcg actccgacga tcgcttgccc
     1081 tcaccccgac cctctcccag ggggagaggg ggctttttcg gatagaacct aagcctctat
     1141 tggtagccag tccagacctg ccttgcggac ggcatagccg aaggtcttta gcacgcgacc
     1201 ataggccagg agccagtgaa accctcgaat ttcctggatt agtcgttgcc aatcccctat
     1261 gatcttcaaa tccagctgag ttcggcaaat gggaaagaat gggctatcca taatggcagc
     1321 ttcgaacccc agccaccgct ggccattgaa gtcgggatca atgacggtca cctcttgtcc
     1381 acggggcatt tccaccttgg gtgcggcccc gtagtccgat tcataatggg tgagaatccg
     1441 tgccggggcc agggatttcc cgtccatacg gcggggggct gtgcagtgag aaaccgtgac
     1501 gatccccttg tgtggaaacg acggattgca gaagaataca ggcctccccg tcacgtaatg
     1561 gagcagaatc cctgccggga tggccaccaa gtcagattcg cagaacgcca aatacccatc
     1621 atcgttgagg agtgttaacg gcaaacaagc cgtagtgcgg gaaaccggca tgatggtcgt
     1681 catgcaattg tggacggtga ttgcattggc gtcagcctca tcgagaagcg tgcgaaagac
     1741 ctcggtcagt acaaatgccc gttccacaaa ccccctctcg gtttccaacg aaacctgggc
     1801 gtcacggaga taggcctctg cctgctgttg acagtgttgc accagacgct cgttcgcccg
     1861 tgcctgtttg agacgctcct ccagctcagg atagggaact gtaatatact cgaatttcca
     1921 gacctctttt gcccgtgcgg gggcctgcgc accgtcggca ccccaaccgg ccgggttgcc
     1981 gatagccaca atccgctgcc ccagggtgtt cttaaccgca aatagggcgc gaagtcgcca
     2041 aaggagttcg ggtaattcat ccaccaccac gtcatcgatg gtcattgccg gttcggcgaa
     2101 gaaatctcgg cgtttccgca gatagtggac atgaactccg atgtacatgt aatagagtgg
     2161 cccactgcgg tgacgcacaa acatcaggtt ccaccgctca gggcgaatca gtgcctcaag
     2221 aaccggcaca ttgcgccgag ccgcgaagac taacatcaca tcgcacggct gtgatgccac
     2281 ggcattggct tcttccacag ttcccacggt gacaaggggc cgaatctcca tgggaaagtc
     2341 agccatctct tgcagcttct tgagatcggc tcggattcga ttctcttctt cccgaagttc
     2401 cgcttcatcc tgaatggcac ccgttacgcg ccagctcgtg gcttcctgac gtgggtaaat
     2461 gtgacaattg aagacgggct gaacttggag tgtcttgaca gagggaggcc ggtggcttgc
     2521 cgtttcccag gcaccgtggg ttgtagttgc caaaacggct gaggccacaa caccgccggc
     2581 ggccgcgccc aaaaagtccc gacgcgtcac gcccgtggtt tgccgatcct ccacgctttg
     2641 cttgctatga ggatatcggc atccacaaca aaagggaaaa tcgcttttgt gctcgaagtt
     2701 cattgtctct tcccctcctc tggcggtacc gtgaatcgca cggcaacttt tcggtcggta
     2761 cgtgacaacg ccgctaacct cgcaggccag ctatattctg gccgtgacct gccaccgatg
     2821 taaatagctt gcaaaaggta gccttgtggc agatcccgca tctggtgtcg atggccaagc
     2881 tgaagaagtg atagccggcc ttcgcatggg ctaggtttga cgttcttggc aagcggaaag
     2941 cctcagtggg gaagatcggg tcagccaggg ggcaaggttg ggtttccgtt gtcgtaagtc
     3001 aacaagacgg ctagattaga gtaaatggcc ggttttgacg cgcatccaac cggtttcaac
     3061 ttgaccagcg ttttcttcgg atttttccgt gatagaagca aacaaggaat gtgatctatc
     3121 cattcgcatt gggggcttgt gctacccaat cctgttatgt tcgcctcggg tacttttgga
     3181 aacatcgtcg atggttaccc gatcgcgaac ctatctcggc ttggggcgat cgtccccaag
     3241 accgtgactt tctctccgcg aataggaaat ccgcccccac gaacggtgga aacaccggct
     3301 gggatgctca attcgattgg gctcgataac gacggtttga tggcctttct ggagcggcgg
     3361 ttgcctgcat tgtccactat cgggccaccg gtgattgtta gtatcgcagc caaagacgag
     3421 gacgaatttg taaaaatggc agagattctc gatgccgcgc aaggtgtgtc ggcagtggaa
     3481 ttaaaccttt cgtgtccaaa cgtagccggc ggcatcgatt ttgcatccga tccgcgaaag
     3541 tgccatgtgc ttgtggcaag ggtcaggcag cgttttagtc gggccatctt ggcaaaactg
     3601 acccccaaca caggcgatat tgcgacgatt gcacgcgctg cggcggacgc tggtgccgat
     3661 gccattacgg cggttaatac cttgttgggc ctggcggtgg actggaggag gcgccgctct
     3721 cgcctgggcg gcttttttgg gggattaagt ggcccggcca tcaagcccgt tgcacttcgg
     3781 tgtgtttacc aaatcgcgca tgccgtggat attccggtga ttgcggcggg cggcgtgaca
     3841 aacgtcgatg atgccatgga gtttttcgtg gtaggcgcct cggccattca ggtgggaacg
     3901 gcatcgttct tccatcccat ggccgcctgg gaaattatcg acgctttgcc ctctgccttg
     3961 cgtgagttgg gggcctattc gattcgggag attgtgaaga cactttgcga acccaggaaa
     4021 gcgtgtgacc ttagaaaaac acgtgagtgc tgagaggagt ctggaataca gtatcgccat
     4081 ccgttgtgaa aatctgtggt ttggtgaggt tcgtttaata atcgggtagt tttatgatcg
     4141 ttgtctcagg aattcagccg acgggtcggt ttcattgggg taattacttc ggtgccatta
     4201 agcagtatgt tgagcttcag gatcaggccg atgcggcgta ttatttcatc gccaatctgc
     4261 atgctctcac cacggtccgt gatccgcagg ttttgcgaca aaacacgatt gacgccgcca
     4321 tcgacctcct ggcattgggt atcaatccgg accgggccac actcttcatt cagtccgatg
     4381 tcccggaagt cacccaactt tgctggctcc tgatgacgtg caccccactg gggcttttgg
     4441 aacgctgtca cgcttacaaa gacaaacggg ctaaaggtct gccggcagac gccgggcttt
     4501 tcacctaccc tgtcctcatg gcagccgaca tcctggccta cgatgcggat acggtgcccg
     4561 tgggcgagga ccaagtccaa catattgagg tatgccgcga cgtcgcctcc agtttcaatc
     4621 atctctatgg caacgtcttc gtgatgccga aggccaagat catggaaaat gccgcgcggg
     4681 tcccgggcac ggatggggaa aagatgtcga aaagctacaa caacacgatc ggcatcttcg
     4741 acgatccgaa gacggtccgc aagaccatca tgcggatcgt aaccgattcg cggcctatcg
     4801 agcaacccaa agccccagaa actgatcatc tgtttcagct ttactctctg tttgtcggag
     4861 aaagggaacg cgaggaaatg gccgctcgtt atcgtcgagg cggatttggc tacggagagg
     4921 tcaaacaggc cctcgccgat gtcgccgaaa agtatttcgc cgaggccaga gaataccgca
     4981 aagagttgga aagtaaaccg cagttcgttc gcgagattct gggcgacggc gcagccatgg
     5041 cccgtaaaaa ggccgcagaa gtcctacaac gggcacagaa tgcctgtggg attaaatact
     5101 gactcttaca agacatgccc ctgatttggc gacatttcgc cgtagcaaat cagaaggcca
     5161 tttgatttgg tccgtccgga gcaaagtaaa atcccgcttg cacggtgcta ctgaaattgc
     5221 tcaaccgaca gggtcaagcc catgcggatc cttggaattg ggaccgatat taccgaatgt
     5281 ctgcgaattg ctcgcatgat tgatcgccac ggggagatct ttctacagag ggtgtacact
     5341 cccgcggaaa tcgcttattg ccaaaaccga aaacaggcaa cgcagcattt tacgggccga
     5401 tgggccgcca aagaggccat c
//
LOCUS       DAOPTR010000099         5420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_12290_length_5420_cov_3.548369, whole genome shotgun sequence.
ACCESSION   DAOPTR010000099 DAOPTR010000000
VERSION     DAOPTR010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12290_length_5420_cov_3.548369"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            415..2319
                     /locus_tag="PKI05_03050"
     CDS             415..2319
                     /locus_tag="PKI05_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOA51204.1"
                     /translation="MLLLIVFAPRVIAWYARQQAEKALHEGRITAAQGWLAWASRCRP
                     ADGRVDILRAGCCRRLKDVEGFVKALQEAEEKKAPPGLIERERRLAAIQAGRLPPGPN
                     NQIGSLITEGYDPAAVAEAFIYGALARGDFAQAWEILSAWSADSPDDGNQNYMAGIYW
                     QFVNQPQKAEEMHRAALRRQPRHELAMLALAEILSTEDRWTELFQLYKVWQRLVPRSK
                     AIRIGLARCARLLGRWDEAWQWLNSDGLSSNLSTARVREIGELLLEMNYPDEAMKWFE
                     RLDIKGTTDRTLLHAVALTESLKGKSLEASQVMDRADRRFQVVTRIHELQMKLTVYPD
                     NHQLRAELENLAKNAPARSPVPDSGSDTTPKSPAQRNSEVRAIYERHCSACHGDQGAG
                     NGRAARFLFPRARNFRRDAFRIVSTEDGIPTVDDVASVIRQGMPGTAMRSFPELSDEE
                     TVQLAELVLEMFREGIREQLVSLYAAEGESVSDEDITEEVLMRTTPGNRVSVPKMGVP
                     DKPILDRGKKAYSLLGCANCHGEDGAGPCDLALWDDQGFPNPPRDLRHGVFKGGDQPE
                     SLYLRLRIGMPGTAHPSCLNTTPEELVSVVHYCRTLIQEPRRTTTNDQRWREAQSRAW
                     YPAYLSPGGP"
     gene            complement(2321..3910)
                     /locus_tag="PKI05_03055"
     CDS             complement(2321..3910)
                     /locus_tag="PKI05_03055"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOA51205.1"
                     /translation="MIKLPTVTLLAGCLFLSCSWIGCRKEVVALPEFDASCVNPSGSD
                     LVRQIELFCGDCHTLPRPEAFPRDRWPAEVRIGYEQYARSGRNDLAPPPIAAVINYYC
                     QQAPEKIAFSLPEDQGGELARHFLSEDVDWNPNENQVPAVSGLLWSSLEPSAQGWLIV
                     SDMREGTVRALAFGQDALAVRLLAHAEFPCRVERCDLDGSGRWGFVIADLGSFFAVDH
                     DRGKVLWLKPSHQEGAYETVTLASRLGRIDDVRVADFDGDGDQDILVAEFGHYHTGGI
                     LFLEQQARSDGQLTFVRHRLDLRTGTIHVPVFDFNGDNRPDFVALISNESESLDLFVN
                     LGKKKFLARPIWRAPDLTFGSSGIELSDLDGDGDQDILYTNGDTFDNLYANPSHGLQW
                     LENIGANGFQYHRLVTLPGAYRALAADLDGDGDQDIVLSSWLSRNVKPANLRTMPIPS
                     VLLLEQINSKEFRVQVLDRDWPQYPVLEVADLDGDGDLDIVAGRHVGLDDPAGVNLPR
                     LRVWWNQKVPGRKPNSDKPSE"
     gene            complement(3995..5131)
                     /locus_tag="PKI05_03060"
     CDS             complement(3995..5131)
                     /locus_tag="PKI05_03060"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOA51206.1"
                     /translation="MVRLVKLRAFTLVELLVVIAIIGILIALLLPAVQAAREAARRSQ
                     CTNNLKQLALAAHNYADVHKVLPPGETGTGTMWGDSTPPADPVNTNTQRLSTWVLLLP
                     YYEQQALYSQISGPLNIGGTVLPAWGRAPDTNDPGNTGNTRFYPPWTTQIDVLMCPSD
                     GKIRQKGANDQGRTNYRTSMGDTIHRSYARDGSVATCRGLFGLREGCPFASITDGTSN
                     TVMFSERLFGGTPALIKEGYATNVTSFNTCTNNLCIPLECVNVRNPARPNEINTTYGT
                     ANWSGRRWACGIPQYTRFNTVLPPNSPACNDSGWDERNAVITASSNHPGGVNVAMADA
                     SVRFVSETIDAGNPALPEVQVGLSPYGIWGALGSKDGGESVAAP"
BASE COUNT         1252 a         1565 c         1478 g         1125 t
ORIGIN      
        1 gcgaactaaa acttctctgg atcaacatgc cgcggcaggg ctaacagaat acgtcgatct
       61 ttcgatgatg cggttcgccc tcgtccttgt caaccatcga ccgtctgtga tcgcgagtaa
      121 atgcgttgta cgccgtctgg cctgacgcta cactgacgcc aatgtggccc gagtgaaccg
      181 agctggtggt caaagacctt tgttattttt tccatcacgc gcactgggcc gacgcccacg
      241 ctggtggccg caatgccgct accaccaatg gttgctcggg atacgtcgcg gtatcgacaa
      301 gcacgtgcat tttaacggaa catgtcttca cgccgatcaa cgacaactca atccgcccgg
      361 aaaaagcctg gcccaaaaag ccgccttttt gctttgggtg tcggtctgat agcattgctc
      421 ttactcatag ttttcgcacc gagagtcata gcatggtacg cacgccagca ggccgaaaaa
      481 gccctccacg aaggccggat cacggcagcc cagggctggc tggcctgggc ctcgcgctgt
      541 cgtcccgcgg atggacgggt cgatattttg cgggcaggat gttgtcgccg cctcaaggat
      601 gtcgagggat ttgtcaaagc cctccaggag gccgaagaga aaaaggctcc gccagggcta
      661 atcgagcggg aacggcggtt ggcggccatc caggcaggac ggcttccccc gggaccgaac
      721 aatcaaattg gctcgttgat taccgaggga tatgaccctg cggcggtggc cgaggctttt
      781 atctacggcg ccttggcacg cggtgatttc gcccaagcct gggaaatcct gtcagcctgg
      841 tccgccgact cgcccgatga cggcaatcag aattacatgg cgggcatcta ttggcaattt
      901 gtcaatcaac ctcagaaagc ggaggagatg caccgggctg ctttacgcag gcagccgcgc
      961 cacgagttgg ccatgcttgc cctggccgag atcctaagta ccgaggatcg ctggacagag
     1021 ctcttccaac tgtacaaagt gtggcagcgt ctggttcccc gaagtaaagc gatcagaata
     1081 ggactggcac gatgtgcccg gctcttgggg cggtgggacg aagcctggca atggcttaac
     1141 agcgatggac tgtcgtcaaa tttgtcaacc gcccgggtcc gagagatcgg cgaacttctg
     1201 ctggaaatga attacccgga tgaagcgatg aaatggttcg agcgactgga tatcaaaggg
     1261 acaacggaca ggacgttgct tcacgctgtc gctttgacgg agtcactgaa gggaaagagc
     1321 ctcgaggcta gtcaggtgat ggaccgtgcc gaccggcgtt ttcaggtggt cacacgcatt
     1381 cacgagttgc agatgaaatt gacggtttat cccgacaatc accagctcag agcggaactg
     1441 gagaaccttg caaagaatgc tcccgcacga tcgcccgtcc ccgattccgg aagcgacacg
     1501 acaccgaaat cgcccgcgca gcgcaattcc gaggtccgcg cgatctatga gcgtcattgt
     1561 tccgcttgcc atggtgacca gggagctggc aatggccgag ccgcacggtt cctgttccca
     1621 cgggcccgca attttcgtcg tgacgccttc cgcatcgtga gtacggaaga cggaatcccc
     1681 accgttgacg acgtggcgag tgtcatccgt cagggaatgc ccggcaccgc catgcgatcg
     1741 tttccggagc tttcggacga agagacggtg cagctagctg agctggtact ggaaatgttt
     1801 cgcgaaggca ttcgggagca acttgtgagc ctttacgccg cagaaggtga aagcgtctcg
     1861 gacgaggaca tcaccgaaga ggttctcatg cgaacgacgc cgggcaatcg agtttcggtt
     1921 cctaaaatgg gcgttcctga taagccgatc ctggatcgcg ggaaaaaggc gtattcgctc
     1981 cttggctgcg ccaattgcca tggggaagat ggcgccgggc catgtgacct cgccctttgg
     2041 gatgaccagg ggtttcccaa tccgccgcgg gacttaagac acggcgtgtt taagggcggc
     2101 gatcaacccg agtctctcta cttgagactt cgcatcggga tgcctggtac tgcccatcct
     2161 tcatgtctta atacgacgcc cgaggagctt gtcagcgtgg tccactactg tcggacccta
     2221 attcaggaac cacgtcgcac gacgaccaac gaccaacgat ggcgagaggc tcaaagcaga
     2281 gcctggtatc ccgcatactt gagccctggg ggaccgtaac tcactcggat ggcttgtcgc
     2341 tgtttggttt tcgtccaggt accttctgat tccaccacac gcgtaaccgc ggtaaattca
     2401 caccggctgg atcatccaaa ccgacgtgac gaccggccac aatgtccagg tcgccatcgc
     2461 cgtctaggtc agccacttcg agcacaggat actgcggcca atccctgtcg aggacctgca
     2521 cacggaactc cttggagttg atctgttcca aaaggagaac cgaggggatg ggcatcgttc
     2581 gcagattcgc tggcttgacg ttccgtgaca accacgacga taggacaata tcctggtctc
     2641 catcgccgtc gagatcagcc gccaaggccc gatacgcccc cggcagcgtt accaaccgat
     2701 ggtattgaaa accattcgct cctatattct ctaaccactg aagcccatgg gaaggattag
     2761 cgtaaaggtt gtcgaaggta tcgccatttg tgtaaaggat gtcttgatcc ccgtctccat
     2821 ccaggtcgct tagctcgatg ccgcttgagc caaacgtcaa gtccggcgca cgccagatcg
     2881 gtcgagccag aaacttcttt tttccaagat tcacaaaaag gtcgagcgat tccgactcgt
     2941 tgctgatcag ggccacaaag tcgggtcgat tgtcgccgtt gaagtcgaac accggaacat
     3001 ggatcgttcc cgtgcgaaga tcaagccgat gacgaacgaa cgttagctgt ccgtccgaac
     3061 gagcttgttg ttccagaaag agaatcccac ctgtgtggta atgtccgaac tctgcaacaa
     3121 ggatgtcttg atcaccatcc ccatcgaagt cggccacacg aacgtcgtca attcgaccaa
     3181 gccgactcgc taaagtgacg gtttcgtagg ccccctcctg gtggctcggt tttaaccaaa
     3241 ggaccttgcc tcgatcatga tcgacagcaa aaaaacttcc caggtccgca atgacaaagc
     3301 cccaacggcc actcccatcg aggtcgcatc gttcgacacg gcaaggaaac tcggcgtgcg
     3361 caagaagacg gaccgccaag gcgtcttgcc caaaggccaa ggccctcacc gtgccttcgc
     3421 gcatatcact cacgatgagc cagccctgag cagacggttc cagactggac cagagcaggc
     3481 ccgacaccgc gggaacctga ttttcattcg gattccagtc aacatcctcg gaaagaaaat
     3541 ggcgagcaag ctcaccaccc tgatcctcgg gtaacgaaaa cgcgattttt tccggtgcct
     3601 gctgacagta atagttaatg accgcagcga ttggcggagg cgccaaatca tttcggccag
     3661 aacgggcata ttgctcgtag ccgattctaa cctccgcagg ccagcggtcg cgcggaaagg
     3721 cttcaggccg tggtagtgtg tggcagtccc cacagaaaag ctcgatttgg cgaaccaaat
     3781 ccgatccaga cgggttaacg caggaagcgt cgaactctgg taacgccacg acctctttgc
     3841 gacagccgat ccacgaacac gacaaaaaca agcaaccggc caagagggtc acggtcggta
     3901 acttgatcat acccacggtc cgtgaccccc atggccagtt gtgtaccttc attgccatca
     3961 tgtgaggcaa ttatggtgga cggtccgcgc cgcatcaagg agcggcgact gactcaccac
     4021 cgtccttgct tcccaaggcg ccccagatgc cgtaggggct caaacccact tggacctcag
     4081 gcaaggccgg atttcccgca tcaatcgttt ccgaaacgaa acggaccgag gcatcagcca
     4141 tggccacgtt gacaccccct gggtggttgc tggaggccgt aatgacggca ttccgttcat
     4201 cccagccact gtcattgcaa gccggactgt tcggtggcaa cactgtgttg aatcgtgtgt
     4261 actgaggaat cccacatgcc cagcgacgcc ctgaccagtt agccgtccca tacgtggtgt
     4321 tgatctcgtt ggggcgagcc gggttacgaa cgttgacaca ctccagggga atacacaggt
     4381 tgttcgtgca cgtattaaag ctcgtcacat ttgtggcata tccttccttg atcagtgccg
     4441 gcgttccccc aaacaatcgc tctgagaaca tgacggtgtt gcttgtgccg tccgtaatgc
     4501 tggcaaaggg acagccctcc cttagcccaa aaagcccgcg gcatgtcgcc acggaaccgt
     4561 cacgtgcgta agaacggtga atcgtatcgc ccatcgaagt ccggtaattc gttcgcccct
     4621 ggtcattcgc acccttctga cgaatcttac cgtccgaggg acacatcaac acatcaatct
     4681 gcgtcgtcca tggtggatag aaacgcgtat tccccgtatt cccaggatca ttcgtatccg
     4741 gcgcacggcc ccaagctggt agaaccgtac cacctatatt gagcggccca ctgatctgcg
     4801 aataaagggc ctgctgttca tagtagggta atagcaagac ccaagtcgaa agccgctgcg
     4861 tgttcgtgtt tacaggatcg gctggcgggg tactatcacc ccacatcgtt cctgttccgg
     4921 tttcaccagg cggcaggacc ttgtggacat ccgcataatt gtgcgcggcc aaggccagct
     4981 gttttagatt gttcgtgcac tgcgaccgcc gagcggcctc tctcgctgcc tgaactgccg
     5041 gcaaaagcag cgcaattagg atgccaataa tggcaatcac cacaagtagt tccacaagcg
     5101 taaacgctct caacttgaca agtctgacca taggtcacct ccatacaaac gagaaaccca
     5161 aagcgaaaaa gttgcaccgg aacaacgctg tagtttacac tccaaactgt tagcacgcaa
     5221 catttttgca gatcccattc gcgataatta atacccatta acaggtgggt taatgcgcta
     5281 attatgggtg gggttgcaat ttcccatggt gacaatacaa tccgacagtg tgcgaaagct
     5341 cgtcaggccg gtcggttcaa aggaggtatt agccatgaag ttcacttaca gtctgatcgt
     5401 gttgctttca tttgcgctcg
//
LOCUS       DAOPTR010000100         5404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_12336_length_5404_cov_3.876986, whole genome shotgun sequence.
ACCESSION   DAOPTR010000100 DAOPTR010000000
VERSION     DAOPTR010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12336_length_5404_cov_3.876986"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PKI05_03065"
     CDS             complement(<1..196)
                     /locus_tag="PKI05_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51207.1"
                     /translation="MKLLQAINTLLSTIVILIILSLLGGGVYLGYSLYESSRSAQQVM
                     EKTLAEKETELAKLSAQLKVR"
     gene            complement(405..968)
                     /locus_tag="PKI05_03070"
     CDS             complement(405..968)
                     /locus_tag="PKI05_03070"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOA51208.1"
                     /translation="MTDHNSKTDQRALELIWAPWRMAYIESCNETVANAVDHSRFLPG
                     ADPGCFLCQAVADPEPPQRLVVYQDDLTVVVLNRYPYNNGHLLVAPRRHVGEFGDVAP
                     PEHTALMGTINRMLSLLKEVMNPDGFNVGLNLGRVAGAGLPGHLHWHIVPRWSGDTNF
                     MPVLANTKVIPQALEALWELLRQKLTE"
     gene            complement(984..2483)
                     /locus_tag="PKI05_03075"
     CDS             complement(984..2483)
                     /locus_tag="PKI05_03075"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HOA51209.1"
                     /translation="MGGKIVFGVAVWLYGAIWCFQPIQGREAAESRSETVVSLQDNGL
                     PLTNPDMGWVLHYYDNVPANYGSRLEPSDTMDDFPSLSVVYLRIPWSYLEPQEGQFCW
                     SVLDTPAQRWISKGKRIALRISCSESWTRFATPEWVQQAGAKGYNFKVGTGVTDDGPY
                     WEPDYNDPVFLEKLDRFLAAMAARYDGNPEVAFIDVGSFGVWGEGHTFASTRIRYPAE
                     TVIRHIELYRKHFKKTLLVANDDFVMTENGEQAIAHALAAGLTLRDDSILVQARPKSY
                     FHAEMAQPFWPKWPVILECEHYGPSRGRGAWEDGTLYLRAVLEYHASYASIHWWPREF
                     LEANRDLIARINRYLGYRLIPTEVRWSSQVALGTPLHWSVTIENRGVAPCYPGGYPAL
                     TLKDEHHGIVAILVNANANVRALPVRQMTLRGPSASNNPEEDLGPPVPTTWSTESCLP
                     FQLKPGRYQVYLSLGTITGTPTIAMPLGNDDGQRRYRVGEVDIIGPAQE"
     gene            2864..4303
                     /locus_tag="PKI05_03080"
     CDS             2864..4303
                     /locus_tag="PKI05_03080"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOA51210.1"
                     /translation="MTKRFLGALATTLILAGVASAGNNVLPELPPLPPLDEISPPEIT
                     APATSPGLILPVGASDWTLPDRLNPSRELVTGMEKQAPAPLPAEPSAPREPVRVTRVC
                     PSSNQDAWTLPQPCFLQNLGIRMGGWLEQGITFNAQDPADHFNGPVATNDLDSEYQMN
                     QLWLWFDRPADNGGCGFAWGGHVDIMYGTDWRFGINHGLEDRINGFDRQTYGMVIPQF
                     YLELAYNRLSVKLGHFAGILDYEAVPGPMNPFYSHSYSYGYTVPQLVTGLLADYKLTD
                     QLSIQGGFHRGWMQFEDNNDELDFMGGVKWKSWNERLTLAYAVSAGAQDDAGDQDRFV
                     YSLVAQYQVNKRFKYVLVHNLGTENNALPNGDDAEWYGINQYFLYTINPCWSAGMRVE
                     WMRDDDGVRIAGPGNIPGVRAWSGRGFAGDFYEITWGLNWRPNGNWTVRPEIRYDWYD
                     GLAGPTGLPFDGGSKDHQLTVGLDAIFTF"
     gene            4593..5123
                     /locus_tag="PKI05_03085"
     CDS             4593..5123
                     /locus_tag="PKI05_03085"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOA51211.1"
                     /translation="MALGEDRVVFQARKFQVVEKRVPNSPEPLRHVIRHRGAVAILAV
                     LDDDRLVFVKNYRISIDAELLEIPAGTREPGESPLEAAIRELKEETGYRAGIWQQLCE
                     FYTSPGITDEKMTLFLAQSLEPGEPQLAPDERLEPCLLTLDEALAVIRSGTIQDAKTL
                     VAVLTYQNMREKDQHN"
BASE COUNT         1258 a         1517 c         1443 g         1186 t
ORIGIN      
        1 cacggacctt aagctgcgcg gagagcttgg ccaactcggt ctctttctcg gccagcgtct
       61 tttccataac ttgttgcgcc gatcgtgagg attcgtaaag ggaataaccc aagtacaccc
      121 caccgcccag gaggctcaag ataatcaaga tgacaatcgt actgagcaac gtattaatcg
      181 cttgcaggag cttcatggtc cgttttggcc tcccaaaagg cccatccagc ggtgtcccaa
      241 gctagtgcga caccaagttc caaatttact tcacaagaaa cgcccaccac agaaagcgag
      301 ttcaagttgt catctctagc caaaacatag catgtggcga tactttctcc aaggtaacgc
      361 ccacctaaag aggggaacac gttggggccc ggcgaaccac cgaattattc agtcaatttt
      421 tgacgcagca actcccagag ggcttccaat gcctggggaa tgacttttgt gttggccagg
      481 accggcatga aatttgtgtc accgctccag cgggggacaa tatgccagtg aaggtgcccc
      541 ggcaatcctg ccccggccac gcgaccaaga ttcagcccca cattaaaccc gtccgggttc
      601 attacctctt tgagcaggct aagcatgcgg ttaatcgtgc ccatcaaggc cgtgtgttct
      661 gggggcgcca catcgccgaa ttctcccacg tgtcggcgtg gggcaacgag aagatggcca
      721 ttgttatatg ggtaccggtt gagcacaaca accgtcaggt cgtcctgata tacgaccagg
      781 cgctgcggcg gttcgggatc ggcaaccgcc tgacagagaa agcagcccgg atcggctccc
      841 ggcagaaagc gagagtggtc cacggcgttc gccaccgtct cattacacga ctcgatatag
      901 gccatccgcc aaggggccca aatgagttcc aaagccctct gatcagtctt tgagttgtga
      961 tccgtcatga cacttccttg atcttactct tgcgcaggac cgatgatatc gacctcgccc
     1021 actcgatacc gccgctgacc atcgtcgttt cccagcggca tggcgatggt gggcgttccg
     1081 gtaattgtgc ctaatgaaag atagacttgg tatcggccag gtttgagttg gaagggcaaa
     1141 catgattcgg ttgaccaggt cgtcggaaca ggtggcccaa gatcttcctc agggttattg
     1201 ctggcactag gaccgcgaag cgtcatttga cgaaccggca aagcccgaac gttcgcgttc
     1261 gcattcacca agattgctac aatcccatga tgctcatcct tgagcgtcag tgccgggtag
     1321 cctcccggat aacagggagc cacgcctcga ttctcgattg tgactgacca gtgaaggggt
     1381 gttcctaggg ccacttggga ggaccaacgc acttccgtag gaatgagccg gtaaccgagg
     1441 tagcggttga tccgtgcaat caagtcgcgg tttgcctcca agaactcgcg aggccaccag
     1501 tgaatcgaag cataactggc atggtactct aaaaccgccc gcaaatagag agtgccgtct
     1561 tcccaagcgc cacgtcctcg cgatgggcca tagtgttcac attccaaaat gacgggccac
     1621 ttgggccaga aaggctgagc catctccgcg tgaaagtatg acttcgggcg cgcttgcacc
     1681 aggatgctgt cgtctcgaag tgttaaacca gccgccaacg cgtgagcgat ggcctgttct
     1741 ccgttttccg tcattacgaa atcgtcattg gctaccaaaa gtgtcttttt aaaatgtttg
     1801 cggtagagct cgatatgccg gattacggtc tcggccggat accgaatgcg agtgctagca
     1861 aatgtgtggc cttcgcccca gaccccaaac gatcccacgt cgatgaaggc cacctcagga
     1921 ttcccatcgt agcgtgcagc catggccgct aagaagcgat cgagtttttc caaaaagacc
     1981 ggatcattgt aatccggttc ccaatatggc ccatcgtctg taaccccggt ccccacctta
     2041 aagttgtatc cctttgctcc ggcctgctgg acccattcgg gcgtggcgaa tcgtgtccac
     2101 gattcggaac agcttatccg cagggcaatt cttttgcctt tggaaatcca ccgctgggcc
     2161 ggtgtgtcca agacggacca gcagaattgc ccttcctggg gttccaggta ggaccaggga
     2221 atccgtaaat aaacgaccga taggctggga aaatcatcca tcgtgtcgga cggttctaac
     2281 cgtgatccat aatttgcggg aacgttatcg taatagtgca aaacccagcc catatcagga
     2341 ttggtcagcg ggagcccatt gtcttggagc gagaccaccg tctcggatcg cgattccgca
     2401 gcttcacgtc cctgaatcgg ttgaaagcac caaatggctc catacaacca gacagcgaca
     2461 ccgaatacga tttttccgcc catatgtttg ttcctcgttt ctcgcggtgg attatccggc
     2521 aaagggtgcg ttcgcctctg tcttaacggt gcacacccta tatgccacaa gagcgatgtt
     2581 tagacagagc gaaagctcgc ccgctccccc cgaaatttcc caccttctct aatcctcata
     2641 tccagacctt aaccacggtc aaaatcgcta ttttccgcgt ggactacaaa aaacgcaaca
     2701 acaaaccgac cgggccaagc cgaaagtttg aatgagcggc ccaatgtcgc cttgcgcaac
     2761 ttttgcggaa tgttcagacg aactggtggc actgagcgtg caaggaccta aacgaatcac
     2821 catcttttcc gcagctagtt ttttaccacg gagacacgct gttatgacta agcgatttct
     2881 cggcgcccta gcaacgacat tgatattggc gggcgtagca tccgctggaa ataacgtgtt
     2941 acctgaattg ccgcccctgc cccccttaga cgaaatcagc ccaccggaga taacggcccc
     3001 cgcgacaagc ccaggcctga tattgcctgt tggggcatcg gattggaccc tgcccgatag
     3061 gttaaacccc agtcgcgaat tagtgaccgg gatggaaaaa caagctcccg cgcctctccc
     3121 ggcggaacct tctgcccccc gggaacctgt tcgggtcacc cgcgtatgtc cctcctccaa
     3181 ccaggatgcc tggacacttc cgcagccttg tttccttcaa aaccttggaa tccgcatggg
     3241 tggatggttg gaacagggta tcacattcaa cgctcaggat ccggccgacc atttcaacgg
     3301 gccggtggcc accaatgacc tggactcgga gtaccaaatg aaccagctct ggctgtggtt
     3361 cgaccgtccg gccgacaatg gcggctgtgg attcgcctgg ggtggccacg tggacatcat
     3421 gtatggcacc gactggcgct ttggaattaa tcatgggcta gaagaccgca tcaacgggtt
     3481 cgaccgtcaa acctatggca tggtcattcc ccagttctat ttggaactgg cttacaatcg
     3541 gctatcagtg aagctggggc actttgccgg gattctcgat tacgaagccg tcccaggccc
     3601 aatgaatccc ttttatagcc attcctactc ttacggttac acagtgccac aactagtgac
     3661 gggcctgttg gcggactaca agttgaccga ccaattgtcg attcagggtg gattccatag
     3721 aggctggatg caattcgagg acaacaacga tgaactggat ttcatgggcg gtgtgaagtg
     3781 gaaatcttgg aacgaacggc tgactttagc ctatgccgtg tccgccggag cgcaagatga
     3841 cgcaggtgat caagatcgtt ttgtctatag cctcgtagca caataccaag tgaataaacg
     3901 cttcaagtat gtcctcgtcc acaacctagg aacggaaaac aacgcccttc cgaatggtga
     3961 cgacgccgaa tggtacggca tcaatcaata tttcctttac acgatcaatc cctgctggtc
     4021 cgcaggaatg cgtgtggaat ggatgcggga cgatgatggc gtccggattg ccggcccggg
     4081 caatattccg ggagtccggg catggagcgg ccgtggattt gccggggatt tctatgaaat
     4141 cacgtggggt ttgaattggc gtcccaatgg caattggacc gtccgtccgg agattcgtta
     4201 tgactggtac gatgggctag cgggtcccac gggccttccc ttcgacggcg gttcaaaaga
     4261 ccatcagttg acggtcggac tggacgccat tttcaccttc taaccttccg gaacgagtgt
     4321 tccctcagag ccagaccgaa ccgcgagtag gtgacggtaa agcgccgcgt cattagcgcg
     4381 gcgctttttg ttgatggcta aatgccaagc aagaatcgtt ccggcggtgt agctcacaag
     4441 ggggcaaaaa gtccaaagcc ttctcgccat gtcggaagtg ccgggcctgt cgcttttcac
     4501 acggccgatg ggcaacgtac actgggggtt catcccggaa aaacagcatg catttttgag
     4561 gacggtcggg agcattctcg cgcgaggatt atatggcttt aggggaagac cgagttgtgt
     4621 tccaggctcg caagtttcag gtggtcgaga agcgggttcc caacagcccg gagccgcttc
     4681 gtcatgtgat tcgtcaccga ggagccgtgg caatcttggc tgtgctggac gatgaccgac
     4741 ttgtgtttgt caaaaattac cgcataagta tcgacgccga gctcctggaa atccccgctg
     4801 gaacccgcga acctggcgag tctccgctgg aggcagcaat ccgggagctc aaggaagaaa
     4861 ccggctaccg tgcaggcatt tggcagcaac tctgtgagtt ttacacatcc cctggaatca
     4921 ccgacgagaa aatgacgcta ttcctggccc agtcgctaga gcctggagag ccgcaattgg
     4981 cacctgacga acgtctggaa ccatgcctcc tgacgttgga cgaggcccta gcggtaatcc
     5041 gcagtggaac catccaagat gccaagacgc ttgtagccgt tctgacctat caaaacatga
     5101 gggaaaaaga tcaacacaat tgatgatatg atcaaatgtt gcgcgacata tgcttatcgt
     5161 ggcgtcctct aagataaact gggtcgaacg tgctcttgat ctggtctgta atccccaagc
     5221 caccagtgca cgttagtgtc tagttgctcg gccaccacag cgcgttggtt ttttgaatct
     5281 atcccaaaaa gctccccctc tccgtctggg agagggctgg ggtgagggca agcgatcgtc
     5341 ggagtcgagt gagaaaaccg gcagcgattc gagccaccct cacccccgac ccctctccca
     5401 ccga
//
LOCUS       DAOPTR010000101         5399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_12347_length_5399_cov_3.562687, whole genome shotgun sequence.
ACCESSION   DAOPTR010000101 DAOPTR010000000
VERSION     DAOPTR010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12347_length_5399_cov_3.562687"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="PKI05_03090"
     CDS             complement(<1..1050)
                     /locus_tag="PKI05_03090"
                     /inference="COORDINATES: protein motif:HMM:NF027656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4962 domain-containing protein"
                     /protein_id="HOA51212.1"
                     /translation="MVCPPVVPLRVLRSIATVATVLFVLLSIAKTQAENVLTTLRREH
                     PRLIVLRTDEERVKKLLSSDPMAREIFQSIQREAESTLKQEPIRHRLIGPRLLDQSRR
                     CLSRMYLLGTSYRLTGDKRFADRAIREMLTAADFPDWNPSHFLDTAEMTHALAIGYDW
                     LYAVMTPEQRQKIREAIVEKGLKEGEKVYRKGTWWTKTPWNWNQVCNGGMTIGALAIA
                     DEEPQLAQYIVEQAVQSLPHAMANYAPDGAWAEGPGYWSYATHYTVYMLAALETALGT
                     DFGLSNQPGFDRAGDFRIHVIGPTRRAFNFADGGDRVGSAPIMLWLAAKFHKPLYAWH
                     ELYYVGSPQSPLHLWW"
     gene            1603..2067
                     /locus_tag="PKI05_03095"
     CDS             1603..2067
                     /locus_tag="PKI05_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51213.1"
                     /translation="MNRLSILSLAAVSLVIVAALAASAGPYDQPCAPPACQPACEQPA
                     CEPACEPACVRVGLVARLRAWCEARRAAAACCKPEACEPEACKPACEPACEPTCEKPA
                     RCCGLLARIRARRAAVECCEVAPAAEQPAPAASPAEAAPAAPQPAPAPAPAT"
     gene            2391..2855
                     /gene="nrdR"
                     /locus_tag="PKI05_03100"
     CDS             2391..2855
                     /gene="nrdR"
                     /locus_tag="PKI05_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOA51214.1"
                     /translation="MKCPYCREDDDRVTDSRIAENGYAIRRRRQCNRCGKRFTTYERV
                     ERRRLKVVKKDGSRVPYDRNKIKTGLEKACWKRPISDEQIESIVTAIEDDIEKNFENE
                     VESRYIGELAMKYLRELDQVAYVRFASVYRQFEDVHDFMEEVRPMLDQARSG"
     gene            complement(2970..>5399)
                     /locus_tag="PKI05_03105"
     CDS             complement(2970..>5399)
                     /locus_tag="PKI05_03105"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOA51215.1"
                     /translation="HLITRYWPLVFVGWGAAFVILHLAAPHWDDVARDGDFAYLPARA
                     ASVQAEKLRAAAFGEANAQSDVVIVMARRDGPLTEQDLDFSEVITNAYQPKEGEESPV
                     VNIIGPRTEVVGKKLLSPISDHGQAALIILLLKGEIMSVENMKPLLAIYEKLDELRRS
                     PQKPPGLELGVTGSGAVGTEMLIAAEESIRNTEVATIGLIIIILLVVYRAPLLAVVPL
                     LAIAAAVFTAFDLLAIIAGISLKTNAFEFAVFKTTKIFVVVVLFGSGTDYCLFLISRY
                     KEELSKGLGTIQAAAKALDGVSAALFASAWTTILGLGMMIFSDFGKFRNGGPAIAVAL
                     IVALAACLTLAPAILCVLGRKIFWPFRVDLSPRDDTDDISLGKRGILGNYSQGLWNRL
                     AGGVLKAPGLMLAGSLLVMAPFVWQAWEVDLTYDLLSELGPDRECVKGTNLLREYFPA
                     GETGPVTVLVYHPDANFNEGPDRYRRLPELVSTIYAMEYTDSAGHRIEPIQTVRSLVD
                     PLGEKSGRSGLFGSLRRGVISGRAKTMYVSQTGPYQGKVARLDVVFPYDPFSIESIRL
                     LDALENYLNEWAAKNPSWQGAAFYFTGTTPGIRDLRAVTLSDQKLIQQLVPIAVLFVL
                     ILILRKPVLSIFLVLSVIWGYYVTLGVTELLFAWLHGATYHGLDWKLPTFLFVILVAV
                     GEDYNIYLVTRVVEEQARFGFEEGLRSALRKTGGIITSCGIIMAGTFIAMISGSLRTM
                     HQLGFALAMGVLLDTLVIRTILVPCVIVLLHKMSRQWRRKEGPPKGESLPLAQQLEPH
                     IENQVPAKTLP"
BASE COUNT         1341 a         1529 c         1414 g         1115 t
ORIGIN      
        1 ccaccacaaa tggagtggcg actgcgggct tcccacgtag tacagttcat gccaggcata
       61 gagcggcttg tgaaacttcg ctgccaacca aagcataatc ggagcggatc ctacgcggtc
      121 cccgccgtct gcaaagttga aggcccgcct cgtcggtccg atgacgtgga tgcgaaaatc
      181 cccagcgcgg tcaaatcccg gttggttcga caaaccaaaa tcggtcccca gggccgtctc
      241 caaggccgcc aacatataga ccgtgtaatg agtggcgtaa ctccaatatc ccggcccttc
      301 tgcccaagct ccatccggtg cataattcgc catggcgtga ggcagcgact gcacagcctg
      361 ttcaacaatg tactgagcta actgaggttc ctcgtcagca atggcgagtg caccgattgt
      421 cataccacca ttgcatacct ggttccaatt ccaaggggtc ttggtccacc acgtaccttt
      481 ccgatacacc ttttccccct ctttgaggcc tttttccacg atggcctcgc gaatcttttg
      541 ccgctgctcc ggcgtcatga ccgcgtacag ccagtcatag ccaatcgcca aggcgtgggt
      601 catttcggcc gtgtcgagga aatgtgaggg attccagtcg ggaaaatcag ccgccgtcaa
      661 catttcccgg atagcacgat cggcaaatct tttgtcgccg gtcaatcgat acgacgtccc
      721 caagaggtac attcgactca aacaacgacg actttgatcg agaagccgag gaccgatgag
      781 gcgatgccga ataggctctt gttttaaggt gctttcggct tctcgctgga tagattgaaa
      841 aatctctcgg gccattggat cgctcgaaag caacttcttc acacgttctt catccgtgcg
      901 aaggacgata agccgtgggt gttcccttcg caaagtagtg agaacgttct cggcctgcgt
      961 ttttgcgatc gaaagcaaga caaagaggac cgtcgcgacg gtggcaatcg agcgtaagac
     1021 ccttaatggc accactggcg ggcaaaccat ggttcactcc ttttctttac cgatcgtaca
     1081 aagcttccag gtgtcacgtt ccccttggaa caaccaaaaa gagaatgatc gagggcatca
     1141 cgggcgcacg tttggcggga ctacccaagt ttgcgtacga ggaacaacca tgcgcttagg
     1201 ttgtgaactg ttccgggtgc ctctaacatc acatgaacat tacatattct ttgcaatcgc
     1261 tcgttagtgc atatctcgtg aacagagtat agaatttgtc ggaaagcgtc aagcaattcg
     1321 accacgataa ttcatcttat tttctccatc caacgcctac ggaacagttt gcctaatcca
     1381 tacccttgac aaaggcgttg aggttgttag gctattcaaa ctaggcggaa agaaaagact
     1441 tcttcgtcgc accgcttttg aacagggcag cggtgagacg aagacggcct aacagcctgg
     1501 tgtgttttgg ccccgcaagc cgtcccgggg tcagacgagt gtcagaggca aagatgacgg
     1561 catgtttcgg agtgaatgtt ctcaaacgag gaggaacaac caatgaaccg tctgagcatc
     1621 ttgagtctag cggctgtgag tcttgtgatt gtcgctgcac tcgcagcaag tgctgggccg
     1681 tacgatcagc catgtgcccc accggcttgc caacccgcct gcgagcagcc ggcatgcgaa
     1741 ccggcgtgcg aacctgcatg cgttcgcgtg ggattagtag cccgcctccg tgcctggtgt
     1801 gaagctcgtc gtgctgcagc agcctgctgc aagcctgaag catgtgagcc cgaagcgtgc
     1861 aaacccgctt gcgaaccggc atgtgagccg acatgcgaaa agcccgcccg ttgctgtggc
     1921 ctgttggccc gtattcgtgc tcgccgcgca gcggttgaat gctgcgaggt agccccggcg
     1981 gccgagcaac cggcacccgc cgcttcccca gccgaggccg cccctgcggc tccgcaaccg
     2041 gcgccagctc cggcaccagc cacctgaaat ggcagaggga aaaggcttcg cttaagtttc
     2101 gggatttctg aagcgaacga aagcccaagt tactataagt gcccagtgtg ccctgttcgc
     2161 tgggacctga cagctacagt gaaccagtcg cgcaccgagc cgatcccgat cagccggggt
     2221 cggcttttta ttgcgctagc cttccggccc ttccaggttt gtttgcctaa tgccacaatg
     2281 aaggggagtg acagacccgt gccaaggacg gggagggaaa acctcggaaa ggcccatcgg
     2341 ccgacagaat ctattggagt ccagggcaat tcctgctcgg aggcaagccc atgaaatgtc
     2401 cctactgccg cgaagacgac gacagagtta ccgatagccg aattgccgaa aatggatatg
     2461 ccattcgacg acggcgacag tgcaatcggt gtgggaagcg gttcaccacg tacgaacgcg
     2521 ttgaacgaag aaggttaaag gtcgtcaaaa aggacgggtc gcgcgtgccg tacgatagga
     2581 acaagatcaa gaccggcctt gagaaggcgt gttggaaaag gcccattagc gacgaacaga
     2641 tcgagtcgat tgtgacggcc atcgaggacg atatcgagaa gaacttcgaa aacgaagtgg
     2701 aaagccggta cattggcgag ctggcgatga aatatctgcg ggaattggac caggtcgctt
     2761 acgtccgttt cgccagcgtg tatcggcagt tcgaggatgt ccacgacttc atggaagagg
     2821 tccgccccat gttagaccag gcaagaagtg ggtgatcgct cgttttgaag tccacaaacg
     2881 atacccttgt gcacgaaacc aatgccgacc gcttggctgt ctgccaacgg catcttgaat
     2941 ctatttcagt acccgtccgt ctcgcgcttc tagggaagcg tttttgccgg aacctggttt
     3001 tcaatgtgcg gctccaactg ctgcgccagc ggaagtgatt cgcccttggg cggaccttct
     3061 ttccgtcgcc attggcggct catcttgtgt aataggacga tgacacacgg caccaaaatc
     3121 gtccggatga caagagtatc caagaggact cccatggcca acgcgaatcc caattgatgc
     3181 atggttcgta acgaaccgct gatcatagcg atgaacgtac ctgccatgat aattccacaa
     3241 ctagtgatga tcccacctgt cttgcgaagc gccgagcgaa gaccttcctc gaaaccaaat
     3301 cgcgcttgtt cttcgaccac ccgcgttacc aagtaaatgt tgtagtcttc tcccacagca
     3361 accagaatga caaataggaa ggtcggcaac ttccaatcca agccgtgata agtcgcgcca
     3421 tgaagccatg cgaacaatag ctcggtgaca cccaatgtca cgtaataacc ccaaatcacc
     3481 gacagaacca agaagatgga aagcactggt tttcgaagga tcagaatcaa aacaaatagc
     3541 acggcaatgg gaacaagctg ttggattaac ttctgatcac tcaacgtgac tgcccgcaaa
     3601 tcccgaatgc ccggtgtcgt acccgtgaaa tagaaggccg ccccttgcca agacggattt
     3661 ttcgcagccc actcattcaa gtaattttca agcgcatcta acagtcgaat gctttcgatc
     3721 gaaaaaggat cataaggaaa aacgacgtct aggcgggcaa ctttgccttg gtagggtccg
     3781 gtctgagata catacatcgt cttggcacga ccgctgatga caccacgccg tagtgagccg
     3841 aatagcccgg atcgcccgct tttctcacct aacggatcga ccaagctccg aaccgtctga
     3901 atcggttcga tccgatgacc ggccgagtcg gtgtattcca tcgcataaat cgtgctgacc
     3961 aactccggca accggcgata acggtccggg ccttcattga aattagcgtc cggatgatag
     4021 acgaggaccg tcaccgggcc cgtctcacct gccggaaaat attcccgaag aaggttagtc
     4081 cccttgacac attcacggtc cggcccaagt tcacttaata ggtcataagt aagatccacc
     4141 tcccaggctt gccaaacgaa aggggccatc acgagaagcg atcccgcgag cataagcccc
     4201 ggtgctttca acacgccgcc cgcaagccta ttccataaac cctgggaata attgccgagg
     4261 ataccccttt tccccaagct aatatcatcc gtgtcgtcac gcggggagag atcaacccgg
     4321 aatggccaaa agattttccg gcccaagaca caaaggatag ctggcgctaa agttagacaa
     4381 gcagccaggg cgacaattaa ggctacagca atcgcaggac cgccgttgcg aaatttccca
     4441 aagtcggaaa agatcatcat gccaagcccg aggatcgtcg tccaggcgct ggcaaatagt
     4501 gcggcgctaa cgccatcaag ggccttggcc gccgcctgga tcgtccccag tcccttactc
     4561 aactcctctt tgtagcgaga aataaggaac agacagtaat ctgttccgct cccgaacagt
     4621 acaaccacaa caaaaatctt tgtcgttttg aaaactgcaa actcaaaggc gtttgtcttc
     4681 agactgatgc ccgcaatgat ggcgagcagg tcaaaggccg tgaacaccgc agcagcgata
     4741 gcgagtaagg gaacgaccgc gagaagggga gcgcgataga ccaccagcaa gataatgatg
     4801 atcaagccaa tggtggccac ctctgtgttt cggatgcttt cttcggcagc gatgagcatt
     4861 tctgtcccca ccgcccctga tcccgtgact ccgagttcga gtcccggtgg tttctggggt
     4921 gaccgcctta gttcatccag cttctcatag atggccaaca aaggcttcat attctcgaca
     4981 gacatgatct cacccttgag caacaagatg atgagagctg cctgtccgtg atcagaaatg
     5041 gggctgagga gtttctttcc aacgacctca gtgcggggac caatgatatt cacaacgggc
     5101 gattcctcgc cttccttggg ctggtaggca ttcgtaatga cctccgagaa atcgaggtcc
     5161 tgctcagtaa gagggccatc tcgacgggcc atgacgataa ccacatcact ttgagcattc
     5221 gcctcaccaa aggccgcggc ccgtagtttc tcggcctgaa cactcgcagc cctggccggc
     5281 agataggcga aatccccgtc tcgggccaca tcgtcccaat ggggagccgc caaatgcaaa
     5341 atcacgaacg cggcacccca cccgacaaaa acaagcggcc aataccgtgt gataagatg
//
LOCUS       DAOPTR010000102         5384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_12392_length_5384_cov_3.724526, whole genome shotgun sequence.
ACCESSION   DAOPTR010000102 DAOPTR010000000
VERSION     DAOPTR010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12392_length_5384_cov_3.724526"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..1419
                     /locus_tag="PKI05_03110"
     CDS             154..1419
                     /locus_tag="PKI05_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HOA51216.1"
                     /translation="MNREKTPAPFDVTELRQQFPALSQRRDGQVCVYFDGAAGTQVPH
                     RVVDAVCRYLIETNANHGGVFATSRESDAILEAARRLLAEFLGTPDSDTIVFGPNMTT
                     LTFALSRSLMKTWKPGDEIVVTDLEHDANVTPWVLAAREVGVNVKSVRINAADGTLEL
                     EDLHSKLSRKTRLLAVTCASNALGTVTPIAEIVHLAHQVGALVFVDAVHYAPHRLIDV
                     MAWDCDFLVCSGYKFFGPHVGVLWGKREWLVSLPCYKVRPAPDEIPYRWMAGTQNHEG
                     IAGLHAALEYLMEIGHRAHPEPSTPRECFAAAFRAIQEHEQELTKVLLEGLAALPEFK
                     VWGISDLTRLRERVPTVAVTHRRLTPKELAEFLALRGIFVWHGNFYALPVTQALGLEP
                     DGLLRISMVHYNTKEEVYRLLAAFRELRD"
     gene            1527..2306
                     /locus_tag="PKI05_03115"
     CDS             1527..2306
                     /locus_tag="PKI05_03115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOA51217.1"
                     /translation="MEVVRQSVYDYPKYYDVLFGSDWKAEYDFLCDCFEYYAKRPVRR
                     VFEPACGTGRLLFRFAQAGYEVAGLDLNEKAVEYCNRRFQRLGLSAHVWVGDMADFRV
                     KRKYDAAFNTINSFRHLTDDKSAMGHLACMREALAKGGLYILGFHLTPNRPPKCTEES
                     WVVRRGHLQVNSWMKTLSLDRQRREEKVYLQFDVYTPRRSFRIVNEFVFRTYTWQQFH
                     RLLQRVGGWEIAGTHDFAYRIDEPITVDDYTEDVVYVLRRV"
     gene            2395..3204
                     /locus_tag="PKI05_03120"
     CDS             2395..3204
                     /locus_tag="PKI05_03120"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOA51218.1"
                     /translation="MTSAQHSQSELERFLRIADMAVDAAAEVLLTKFGRVEAQLKGYA
                     DFVTEADMGAQQAVVEIIHREFPHHDFLGEEGSRGGSSQSSYRWILDPLDGTTNYVHG
                     VPFFAVSLALEYEGRPIVGIIYDPMLRERFTAVTGWGAQLNGQPIRVSHVKRLSDALV
                     SIGLPARVVPDSVDVHWMIQSAVHCLSIRRTGSAALNMAYLAAGRFDISWALTTKAWD
                     VAAGAILIEEAGGVVTGLDGQPLNAQGGPYVAAANRDLLDQFLAVVGPFRK"
     gene            3315..4877
                     /locus_tag="PKI05_03125"
     CDS             3315..4877
                     /locus_tag="PKI05_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51219.1"
                     /translation="MSHEHTTPHGKIRPHCCPCCDSSVPINLGEFTRRTFLGGATLGT
                     ALFAGLTWAKLAEETPELPMPPGRLPLKVKPVFLYHVYQPRPQTSWRPWGGIQTEEAA
                     AEEVKRIQGELEKLQAAADFPMEIAPLTATKNAAELAQMPDVQSADVLLVYANDGDLN
                     AIAALKKDTIFFIRHKSGPVYLYYEIISPRFLRQHTDELKVPGMDNDDVVIDNYDEVL
                     WRLRALCGLKNARNQRIIAVGGAGAWAQPPKVVPELVKKIWNTEIIDLPYPELGKMIQ
                     AARSDQAAVNLAKRRAEEYLKDPMVKLETDRTYVENAFLLDQVFRKVMAQANCRMITI
                     HGCMGTIMPLAETTACLTLSTLNDDGYLAFCESDFVVIPSGMLLCGISGLPPFLNDPT
                     YPHDKIITLAHCTAPRKMNVKEKEPARLLTHFESDYGAAPKVEMRNGQVVTNILPDFK
                     SERWVGLRGEIVAHPFLPICRSQIDIKYTCDDKILADRMPGFHWMTIYGDYIREAGYA
                     LKRVGIKFEVLG"
     gene            complement(4963..>5384)
                     /locus_tag="PKI05_03130"
     CDS             complement(4963..>5384)
                     /locus_tag="PKI05_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sialidase"
                     /protein_id="HOA51220.1"
                     /translation="AIGHSLGGKEVLYLAAFDERIRATVSSEGGVGLSFSNWDAPWYL
                     GPEIREPQFGHDHHELLALIAPRAFLLIGGDSADGEKSLPYLQSAWQVYRIYTERPAL
                     GLWNHRQGHSVPHEAEQRIAEWFDVYLREKVISPSTE"
BASE COUNT         1140 a         1515 c         1527 g         1202 t
ORIGIN      
        1 ggggtcgggg ttgagggtgg ctcgaatcgc tgccggtttt ctcgatggac tccgacgttc
       61 gcttgccctc accccaaccc tctcccggag ggagaagggg actttttagg ataggctgta
      121 agtcggtaat aggcctttga tgaacggatt accatgaatc gcgagaagac ccctgcacct
      181 ttcgatgtca cagaattgcg gcaacagttt ccggccttgt ctcaacggcg ggacggtcag
      241 gtgtgcgttt attttgacgg ggcggcaggg acgcaggttc cgcatcgggt tgtggatgcg
      301 gtgtgtcggt acctcatcga gaccaacgcc aatcacggcg gcgtctttgc cacaagccgg
      361 gagagcgacg ccatcctgga agcagcacgc cgattgctgg ccgagtttct cggcacgccg
      421 gattcggaca cgatcgtttt tgggcccaac atgacaacct taacctttgc cctctcccgg
      481 tcgctcatga agacctggaa acccggggac gaaatcgtgg tcaccgatct ggaacacgat
      541 gccaacgtga cgccctgggt cctcgccgcc cgagaggttg gagtcaacgt taagtcggtg
      601 cggatcaatg ccgcggatgg cactcttgag ttggaagacc tccactcaaa gctgagtcgc
      661 aagacacggc ttttggccgt cacatgtgct tctaatgccc tggggacggt aacaccgatt
      721 gccgaaatcg tccatctcgc ccaccaggtt ggtgcccttg tctttgtcga tgccgtccac
      781 tacgcaccgc accggttgat tgatgttatg gcgtgggact gcgattttct ggtctgttcg
      841 ggttataaat tctttgggcc tcatgtgggc gtcctttggg gaaagcgcga atggctcgta
      901 tcgctgccct gctacaaggt gcggccagca ccggatgaga tcccctatcg ctggatggcc
      961 ggcacgcaaa accacgaagg aattgccggc ttgcacgccg cacttgagta cctcatggag
     1021 atcggtcacc gggcccaccc ggaaccgagt acaccgcggg agtgttttgc cgctgcgttt
     1081 cgggccatcc aagagcacga acaagagctg accaaggtcc tgttggaggg cctggccgcg
     1141 ctgcctgagt tcaaagtttg gggcataagt gatcttacgc gactccgcga acgcgtgccc
     1201 acggtggccg tcacccatcg gcgactcacg cccaaagaat tggcggagtt cctggccttg
     1261 cgcggcatct ttgtgtggca tgggaatttt tacgcacttc cggtcacgca agcactgggt
     1321 ttggaacccg atggcctgct caggatttcg atggtccatt acaataccaa ggaagaggtc
     1381 tatcggctct tggctgcctt ccgcgagttg cgcgattgaa ccaaaccgac aaggcgcggg
     1441 gactcatagc gaggctagaa cggggtttgc gtcgtcccgg ccgtccaagt ttggctgttc
     1501 ctcgcttcgg agcttttgga agcactatgg aagttgtccg gcaaagtgtt tatgactatc
     1561 ccaaatatta cgacgttctc ttcggttcag actggaaagc cgagtacgac ttcttgtgcg
     1621 actgctttga atactatgcc aagcgtccgg tcagacgggt attcgagcct gcctgcggaa
     1681 ccggaagatt actcttccgt tttgcccaag cgggatacga agtcgccgga cttgatctca
     1741 acgagaaggc cgtcgaatac tgtaaccgca gattccagcg tttgggcctc tcggctcatg
     1801 tctgggtcgg cgatatggca gattttcgcg ttaagcgcaa gtatgatgct gccttcaaca
     1861 ccatcaacag ctttcgtcac ctaactgacg acaaaagcgc tatgggacat cttgcctgta
     1921 tgcgggaagc gttggccaaa ggcggtcttt acatcttggg ctttcacctg acccccaatc
     1981 ggccaccaaa gtgtaccgag gaatcctggg tcgttcgacg cggccacctt caggtcaact
     2041 cctggatgaa aacactgagc ctcgaccggc agcggcggga agaaaaagtt taccttcagt
     2101 ttgacgttta tacgccccgt agatcctttc gcatcgtcaa cgagttcgtc ttccgcacat
     2161 acacgtggca acaattccac agacttctgc agagggttgg gggctgggaa attgcgggca
     2221 cccacgattt tgcataccgg attgatgaac cgatcactgt cgacgattac accgaagatg
     2281 tggtgtacgt cttgcgccgg gtgtaggaac tagcacgacc tccgtcgcaa gattgataca
     2341 cacaaagcac accagccaaa aaggatttga aacctgtttc actgagagga aagcatgaca
     2401 tcagcgcagc acagtcaatc ggaactagaa cgtttcttac gaattgccga catggccgtc
     2461 gatgcggcag ccgaggtgct tctcaccaaa ttcggtcggg tggaagctca attgaaaggc
     2521 tacgccgact ttgttactga ggccgatatg ggtgcccaac aggccgttgt cgagatcatt
     2581 caccgcgagt ttccccatca cgactttctc ggtgaagaag ggagccgggg gggatcgtct
     2641 cagtcgtcat accgctggat tctagaccct ctcgacggta cgacgaatta cgtccacggt
     2701 gtgccctttt tcgccgtctc gctggctctc gagtacgaag ggcggcccat cgtgggcatt
     2761 atctacgatc ctatgctccg ggagcggttt acagcggtta ccggctgggg ggcacaactg
     2821 aatggtcaac cgattcgggt cagtcacgtc aaacgattga gcgatgccct cgtctccatc
     2881 ggacttcctg cccgcgtggt tcctgattcg gtggatgttc actggatgat ccaatccgcc
     2941 gtgcattgcc tatccatccg acggacgggg tcggcagccc tgaatatggc ctatctggcg
     3001 gcggggcggt tcgatatctc atgggcgctg acgaccaagg cttgggatgt tgcggcggga
     3061 gccattctca tcgaagaggc agggggcgtg gtcacgggtc ttgatggcca gcccttgaat
     3121 gcccagggtg ggccgtacgt ggctgccgcc aatcgcgatc tactggatca gttcctcgcc
     3181 gttgtcgggc cgtttcgcaa atagtcacgc tttgagttgc aaccgcccct gaggaacgtt
     3241 atcctaaaag cgccgcttgg gattagttcg gccatgtccg tttgatattt ggtcagtcac
     3301 cgacgaggga gtccatgtct cacgagcaca ccacgccgca cggaaagatc cgtccgcact
     3361 gttgcccatg ctgtgattcg tcagttccga tcaacctcgg cgaattcaca aggcggacct
     3421 ttttaggagg ggccactttg ggcacggccc tgtttgccgg tctgacttgg gcaaagctcg
     3481 cggaggaaac gcctgagctg cccatgcccc ctggcaggct tcctctgaag gttaagcccg
     3541 ttttcctgta tcacgtgtac cagccgcgtc cccaaacgag ttggcgtcct tggggtggaa
     3601 ttcagaccga agaagctgcc gcagaggagg tcaaacggat tcagggcgaa ctcgagaaac
     3661 tccaggcggc tgcggacttc cccatggaaa ttgcaccgct caccgcgact aaaaacgccg
     3721 cggaattggc tcaaatgccg gatgtccaat ctgcggacgt ccttttggtg tatgccaacg
     3781 acggcgattt gaacgccata gccgctctca agaaagacac catcttcttt atccgacaca
     3841 agtccggccc agtgtatctt tattacgaga tcatcagtcc tcgtttccta cgtcagcaca
     3901 ccgacgagct gaaagtcccg ggcatggata acgacgatgt cgtgatcgac aattacgatg
     3961 aagttctctg gcgcctgcgc gcgctttgtg ggctgaaaaa tgctcgtaat caacggatca
     4021 tcgcagtagg tggagccggg gcgtgggccc aacccccgaa ggtggttccc gaactcgtca
     4081 agaagatttg gaacacagaa atcattgacc tgccctatcc agaattgggc aagatgatcc
     4141 aggcagcccg ctccgatcag gcggccgtga acctggccaa acggcgagcc gaagagtatc
     4201 tcaaggaccc aatggtcaaa ttggagacag atcgcactta tgtggaaaac gcgtttcttt
     4261 tggatcaagt tttccgcaag gtgatggccc aagccaactg ccggatgatt accatccacg
     4321 gatgcatggg aacaattatg ccgctggccg aaaccacggc ctgtctcacg ctgagcaccc
     4381 tcaatgatga cggttacctg gcgttttgcg aatcggactt cgtggtgatt ccttcaggga
     4441 tgctcctctg tggaatttct ggcttgccgc cgttccttaa tgacccgacc tatcctcacg
     4501 acaagatcat cacgctggcc cattgcacgg ccccacggaa gatgaatgtc aaggagaagg
     4561 agcccgcgcg acttctcacg catttcgagt cggattacgg tgctgcgcct aaagtcgaaa
     4621 tgcggaacgg ccaggttgtt acaaatatcc tgccagactt caaatcggaa cgctgggtgg
     4681 gcctgcgggg cgagatcgtt gcccatcctt tcctgcccat ttgccgttcg caaatcgata
     4741 tcaagtacac ctgcgacgac aagatcctgg ccgacaggat gcccggcttc cactggatga
     4801 caatctacgg cgattacatc cgtgaagcgg gctacgccct gaaacgggtc ggcatcaagt
     4861 tcgaagttct cggctgatgc aatcgagtct ttcgacgctt ctacgggccg caccgtcctc
     4921 tgggcggtgc ggtcttttct ttaagagcag cttaaggtac tgctattccg tggatgggga
     4981 aatgaccttt tcccgcagat agacatcgaa ccattctgcg atgcgttgct cggcctcatg
     5041 gggaacgctg tggccttgcc ggtgattcca taggcccaaa gccggccgct ctgtgtagat
     5101 tcgatagact tgccacgccg attgcagata cggcagactt ttttcaccat ctgccgagtc
     5161 gccaccaatc aaaagaaacg cccgcggggc aatgagggcc aggagctcat ggtggtcatg
     5221 tccaaactgt ggctcgcgaa tctctgggcc taaataccac ggggcgtccc agttggagaa
     5281 acttagcccg acaccgccct cgctcgaaac cgtggcccta atccgctcgt cgaaggccgc
     5341 taggtagagc acttcctttc cgcccagaga atggccaatg gccc
//
LOCUS       DAOPTR010000103         5361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_12453_length_5361_cov_3.837354, whole genome shotgun sequence.
ACCESSION   DAOPTR010000103 DAOPTR010000000
VERSION     DAOPTR010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12453_length_5361_cov_3.837354"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..1352)
                     /locus_tag="PKI05_03135"
     CDS             complement(141..1352)
                     /locus_tag="PKI05_03135"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOA51221.1"
                     /translation="MARSFRQAAVSRRFLAGTGGRVTRRNLIKGLGAAVTLPWIGKAL
                     AALSPKAEILEVKTISRLPHRYHGWPTVARRKNGQLVLVYSGGREAHVCPFGRVEMMT
                     SNNDGDWWTYPRVLIDSAIDDRDAGVLETAKGTLLVTTFTSLAYVPILERAMADPGKG
                     WPSEKIDRWRAAHERLTEADRKAQLGVWMIRSEGGGVTWSSPYDCLVDSPHGPIQLRD
                     GRLLYAGKDLWRTENVGVCVSEDDGRTWQWLAVLPTREGDNPKQYHELHAVETTSGRL
                     IVQIRNHNPANNGETLQSESEDGGKTWSVPHPIGVWGLPSHLLRMVDGRLLMSYGHRR
                     PPFGCQARISEDEGKTWSEPIVIYGDGAGGDLGYPSTVQLAGGDFVTIWYELMSDSPF
                     AVLRQARWRLA"
     gene            complement(1476..2006)
                     /locus_tag="PKI05_03140"
     CDS             complement(1476..2006)
                     /locus_tag="PKI05_03140"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOA51222.1"
                     /translation="MNQFLLQQIRRRHILREAEGYLELGLFERALECLDRLPDRDRQS
                     FEARLIRAEILRAMGQYESALEIYESLIAQRPDDLNILLAMGWCHKRIGRIDLACADL
                     EKAKQISPRNSLVLYNLACYYSLAGRKQDALTHLEKALQLEPGLRKLIDTETDFDPLR
                     SDSDFIRLVYPDKGNR"
     gene            2388..2756
                     /gene="yajC"
                     /locus_tag="PKI05_03145"
     CDS             2388..2756
                     /gene="yajC"
                     /locus_tag="PKI05_03145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOA51223.1"
                     /translation="MTPGSLLFLAEAASGNTSSGVLMMLTLWIPLILVFYFLLIRPER
                     KERARRQAMLQALKKNDRVLTIGGIYGVVTNVHREANEVTLRVDDATNTRIRCTLSAI
                     AQVLGEETSAGNGKDAKSGD"
     gene            2875..>5361
                     /gene="secD"
                     /locus_tag="PKI05_03150"
     CDS             2875..>5361
                     /gene="secD"
                     /locus_tag="PKI05_03150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOA51224.1"
                     /translation="MDRRVTSLRRFGLTLGVPVVFAIFALVLAAPLPVGSSARAEQAP
                     AVAEGPQLPAASVTNQGENAQSSGTVAPETPAPSEKVAPSADQEQPEKQTAVATAQET
                     PAPGGQTSPAPQAAEKAPGEGQLSGLLNALIYLGFLLLIIGLPVGLAYFICEQWRLRE
                     YFGRLSFVFFTIVVAIVIILTRWPPKLGIDLSGGVIMVYEIEGAVRQPGERAQADRDQ
                     IDMDQLIAALNRRVNPSGVKEVTIRRFGPQQIEIIVPEVNEEEVARLERVISSIGTLE
                     FRILANQRDHRRLIERAMALSPDEQELYDDRGNLLAWWVPVAKGQEAAIGTGGEIAVR
                     MGRYRNEDWLQVLVVKDRYNVTGADLFRVSEDVDERMQPCVSFVLRAGSAQRFGALTS
                     ENRPDATTGFERRLGIILDGYLYSAPNLRQPIFDRGQITGIPTREEVQALIQVLQAGS
                     LPAVLSPTPISRLVTGPQLGRDTIRQSVTAMIVAAVIVFAFMVAYYRFAGIVACLAVV
                     LNLVMLVAIMILLKAAFTLPGLAGLTLTVGMAVDANVLIYERIREELSRKATLRMAIR
                     NGFEKAMSVIIDSNLTTMLTAVVLYFVGTDQVRGFAVTLFLGLVLNLYTAVFMARIIF
                     EIGERRKWIKQLYMMRIIGETNFDFTGVKKYALAGSVAVILLGLVALIARGPGVLDID
                     FTGGVAVEILFNKPKDIGEVRYRLRELRDLAVSDVRIEGEQPHRRYVINTSSPPGVEA
                     EQYLRSVKEKIAQVFGDELVHYSVQIENLSQEGTTYVPRRGFPNLMLAGSPWGSALAG
                     VLVGASGEETSQTGEAGQSGPATEQGPKLPE"
BASE COUNT         1157 a         1444 c         1525 g         1235 t
ORIGIN      
        1 atgcctgttc ggcgtgcgtg cttaagatat tttcgaataa caacttgcgc gcacaagatg
       61 cgcgaaacat ctgccacaat ggcagcgttg gacgtccctt ccaaggtggc gttaccggtt
      121 tgcgtgatga caaccccgcc ttatgccaat cgccaacggg cttgccgcaa aacggcaaac
      181 ggagaatcgg acatcaactc gtaccaaatc gtcacaaagt cacccccggc caattgcacc
      241 gtgctcgggt atcccagatc tccgcccgca ccatccccgt aaatgacgat gggttcagac
      301 catgtcttgc cttcgtcctc gctgattcgg gcttggcagc cgaaaggtgg acgtcggtgt
      361 ccataactca taagaagacg accatccacc atccgtaaca aatgggaagg caatccccaa
      421 acgccgatgg gatggggaac gctccatgtt tttccgccgt cctcggattc cgattgcagg
      481 gtttcgccgt tattggcggg attatgattg cggatctgaa cgattaaccg accgcttgtc
      541 gtctccaccg catgaagttc atggtattgc ttgggattgt caccctccct cgttggtaaa
      601 acggcaagcc actgccaagt ccgcccgtca tcttccgaga cgcagacacc aacgttctct
      661 gtccgccaga ggtctttccc ggcgtagagg aggcggccat cccgcaactg aatgggacca
      721 tgcggactgt caacgaggca atcgtacgga gacgaccaag tcacgccccc gccttcagag
      781 cggatcatcc acacgccaag ttgtgccttg cgatcagcct ccgtcagccg ttcgtgagct
      841 gcccgccatc gatcgatctt ctcagagggc cagcccttgc ccggatcggc catcgcccgc
      901 tcgaggatcg gcacgtacgc taacgaggta aaagttgtga cgagaagtgt tccctttgcc
      961 gtttcgagaa cgccggcatc tcggtcatca atggccgaat cgatcaaaac ccgtgggtat
     1021 gtccaccaat cgccgtcatt gtttgatgtc atcatttcca cgcgaccgaa cgggcataca
     1081 tgggcctcgc gaccaccgga atataccaag acgagttgac catttttgcg tcttgccacg
     1141 gtgggccagc cgtggtaccg gtggggcaaa cgactgatcg ttttaacttc gaggatctca
     1201 gccttcggtg agagagcagc caaggccttt cctatccagg gaagtgtgac cgcagccccc
     1261 aagcctttaa tcagatttcg ccgagtgacc cttccgcctg tgcctgccag aaaacgtcgg
     1321 gaaaccgccg cctgcctgaa agaacgtgcc atggcaaatc gctccaatct taaataatga
     1381 aaacccatgg gccgctggtg caccacaagc agcggtggct gcagtgcatg gcagcctcca
     1441 ccgctacgac gattgcttga ccgacaccag acaatttagc ggtttccctt atcggggtaa
     1501 accagccgga tgaaatcgga atcagatcgc aagggatcaa aatccgtttc tgtgtcgatg
     1561 agtttgcgca gcccaggttc cagttggaga gccttttcga ggtgagtcag ggcgtcctgc
     1621 tttcgccctg cgagactata atagcaggcc aaattgtaga gcactaagga gttgcgagga
     1681 ctaatctgtt ttgccttttc caggtcggcg caagcaagat cgatgcgtcc gattcgttta
     1741 tggcaccagc ccatcgccaa caaaatgttc aggtcatctg gccgctgggc aatgaggctt
     1801 tcgtaaatct ccagcgccga ctcgtactgt cccattgcac gaagaatctc ggcgcggatg
     1861 agacgggcct caaaagattg acgatcccta tctggcaaac gatctaggca ctccaaggcc
     1921 ctttcgaata gccccaattc aagatacccc tccgcctctc gcaagatatg ccttcgacga
     1981 atctgctgaa ggaggaactg gttcatacgt tgttccaaac tttaggatgt ccagatttaa
     2041 agtgacgtta tcgtcgaaca cccctacttt cattttatgc cttcgctttt gcccagaagg
     2101 ggggctgatg gtggatcacc tttcccacgg agacggtggg gcagtgctca ggagaggaca
     2161 gaattgctct atccccatgg gtgtgttgat gctgggttga cctcctcacc cctttgcagc
     2221 tccccagctt gcccgcattc cccttgtggc attacctttc aagctttaag attcattatt
     2281 tggcggttaa aggcatgcaa cccccatcag tgggacagat gtgtgatgat tggcattgtt
     2341 caattggcat gtcgccagcg gggtactgac caagtcagga gaggccaatg acgccgggta
     2401 gtctgctttt tcttgccgag gcggcatcgg gcaacacgtc tagtggtgtc ctgatgatgc
     2461 tgaccttgtg gatcccgctg atccttgttt tttatttttt gctgatccgt ccggaacgga
     2521 aagagcgcgc acgacgccaa gccatgttgc aggcgctcaa gaaaaacgac cgtgtactca
     2581 cgattggtgg catctatgga gtcgttacca atgttcaccg ggaggcgaat gaggtgacat
     2641 tacgggtgga tgatgccacc aatacacgta ttcgttgcac cttgagtgcg attgctcaag
     2701 tgttgggaga ggaaacgtcg gcgggtaacg gcaaggacgc caaatcaggt gactaggcca
     2761 gaacgttccc gcggcattgg gggatgatgg gatacgtcaa gtccctcttc attgtgaaat
     2821 aggggaatta ttgccaggcg cggatgattt ttgtgcattg agcgagttaa gacgatggac
     2881 cgacgcgtga cgtctttacg gcggtttggg ttgaccctcg gtgtcccggt ggtttttgca
     2941 atcttcgccc tcgttcttgc ggccccgctt ccagtgggtt cgtctgcccg ggcagagcaa
     3001 gccccggcag tggctgaggg gccacaatta ccggcggcct cggtgacgaa tcaaggtgag
     3061 aatgcccaat cttctgggac ggtagccccg gagacccctg ctccatccga gaaggtggca
     3121 ccatcggcag accaagagca gccggagaaa cagactgctg tggctactgc ccaggaaaca
     3181 ccagcaccag gtgggcagac gtctccggcc ccgcaagcag cggagaaggc accaggggaa
     3241 gggcaattat ccggactcct gaacgcactg atctatcttg gattcctact tttaattata
     3301 gggctacccg tcggcctggc ttacttcatc tgcgaacaat ggcgacttcg cgagtacttc
     3361 ggccgacttt cttttgtctt tttcacaatt gtcgttgcta tcgtgatcat tctcacacgc
     3421 tggccgccaa agttggggat cgacctcagc ggtggagtga tcatggttta tgaaatcgag
     3481 ggcgccgtgc gacaaccggg cgaacgggcc caggccgatc gtgatcagat cgatatggat
     3541 cagttgattg cggccctcaa tcggcgtgtc aatccgagcg gtgtcaagga agtgacaatt
     3601 cgtcgctttg ggccgcagca gattgagatc atcgttcccg aagtaaatga agaggaagtc
     3661 gctcggttgg agcgagtgat cagcagcatc gggaccttgg aattccgcat ccttgccaat
     3721 caacgggatc atcgccgctt gattgaaagg gcgatggcgc tttccccgga tgagcaagag
     3781 ttgtacgacg accggggtaa tcttctcgcc tggtgggtgc ctgtggccaa aggccaggag
     3841 gcagctattg gcactggcgg agaaatcgcc gtccgcatgg gtcgatatcg aaatgaagat
     3901 tggctccagg tcctggtggt caaagatcgc tataacgtaa ccggagccga tctcttccgg
     3961 gtttcggaag acgtcgatga aaggatgcaa ccgtgtgtgt cgttcgtttt gcgagcggga
     4021 agcgctcagc gtttcggcgc gttaacgagc gagaaccgcc ccgatgcgac cacgggattt
     4081 gagcgacgtt tggggattat cctcgacggt tatctctatt ccgcaccaaa cttgaggcag
     4141 ccgatttttg accgaggcca aattacgggc atccccaccc gcgaagaagt tcaagcttta
     4201 attcaggtct tgcaggccgg cagtttgcct gcggtcctca gcccgacgcc tatcagccgc
     4261 cttgtcaccg gtccccagtt gggccgcgac acgatccggc agagtgtcac ggccatgatc
     4321 gtggcggccg tcatcgtctt tgccttcatg gttgcgtact accgatttgc cgggatcgtg
     4381 gcctgcttgg cggttgttct caacctggtg atgctcgtcg caatcatgat tcttctaaag
     4441 gcggctttca ccctgcccgg gcttgcaggg ttaacgttga cggtcggtat ggccgtggat
     4501 gcgaatgtgc tcatctatga acgaatccgt gaagaattat cgcgaaaggc taccctgcgg
     4561 atggccatcc gcaatgggtt tgaaaaggcc atgagtgtta ttatcgactc caacttaacg
     4621 acgatgctca cggccgttgt gctctatttc gtggggaccg accaggtccg cgggtttgcc
     4681 gtcacgttgt ttttggggtt ggtgctcaac ctttatacgg ctgtgttcat ggcccggata
     4741 atttttgaga ttggtgaacg ccgtaaatgg attaagcagc tctacatgat gcggatcatt
     4801 ggcgagacca attttgattt cacgggcgtg aagaaatatg ctttggcggg aagtgtcgca
     4861 gtgattctat tgggactcgt tgccctaatc gcacggggac caggtgtgct ggatatcgat
     4921 tttacgggcg gagtcgccgt tgagattctg ttcaataaac cgaaagacat tggcgaggtt
     4981 cgttatcgct tgcgagaact tcgggacttg gcagtcagcg acgtgcgaat tgaaggcgag
     5041 cagccgcacc ggcgatacgt cattaacaca tcaagtccgc caggagtcga agccgaacag
     5101 tacctccgga gtgtcaagga aaaaattgcc caagttttcg gcgacgagtt agtgcattat
     5161 tccgttcaga ttgaaaatct gtctcaagag gggactacct acgtgccgcg aaggggtttt
     5221 ccgaacttga tgctcgccgg tagcccgtgg ggttcagcgc tggccggtgt gctcgtgggg
     5281 gccagcggcg aggagacctc tcagaccggg gaggctggcc agtcgggtcc tgccaccgag
     5341 caggggccca aactgccgga g
//
LOCUS       DAOPTR010000104         5346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_12501_length_5346_cov_3.734644, whole genome shotgun sequence.
ACCESSION   DAOPTR010000104 DAOPTR010000000
VERSION     DAOPTR010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12501_length_5346_cov_3.734644"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1591
                     /gene="ilvB"
                     /locus_tag="PKI05_03155"
     CDS             <1..1591
                     /gene="ilvB"
                     /locus_tag="PKI05_03155"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOA51225.1"
                     /translation="RHEQGGGFAAQGYARSTGKVGVCMTTSGPGATNVITALADAKLD
                     SVPIVAITGQVGTSVIGTDAFQETPTVEVCRSITKHHYLVTDVNDLARVFREAFYIAQ
                     TGRPGPVLIDIPKNIQLAQTVPDYNAPMDLPGYRPVVRLARPEEIREIAQVIKKSRRP
                     VVYAGGGVITSGASEALREFVEKTSIPVAMTAMGLGGFPGDHPLSLDMLGMHGSIYAN
                     YAVAECDLLLAFGVRFDDRVTGKVSEFAKHATIVHIDVDPSELNKVKPAHIAVVSDLK
                     YALEELNKIVEPPDISEWVTRVMERKKAEPFSYREYEDAILPQQAIHELWKLTKDQDT
                     IITTGVGQHQMWTAQFYKFRKPRTWISSLGLGTMGFGLPAAIGAKVAHPDKIVIDIDG
                     DGSLLMNIQEMATAYCEKIPVKVLLLDNQHLGMVVQWEDRFHARNRAHTYLGPIDQPE
                     AVGRGEGKIPRYHYPDFVTIAKGFNWRARHIRHKSELIDGLKEMLASEEPYLLDVLVP
                     YQEHVLPMIPAGMTIKELIRE"
     gene            complement(1868..3541)
                     /locus_tag="PKI05_03160"
     CDS             complement(1868..3541)
                     /locus_tag="PKI05_03160"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOA51226.1"
                     /translation="MKSFLVLTVPATVICVLGWLFSIASAEDASQQPVAISTFHCIGL
                     YWSPPGGSPDRQVLVRFRRQGEEAWQQGLPMRYNPIPKTDQDLADYRGSLVHLTPGTK
                     YEIELQLEGTDQVARLVASTWSEEFPIGEVINVPGGKEPLAITESGRPDAYRVYDGQG
                     AVIDVEHKHDTCISIDASYVIVRNFVLKGAGATNNPRGSPIGAIRIDKGHDIVIEDCD
                     ISDWGRTNPKTGFGFDYDAAVLCRNRDVHRVIIQRCRMHHPFSDTNTWYEPVYPTHPQ
                     GPQCISFFDTAGNHVIRYNEMYSDLDHMYNDIVGGGSNGSYRGSPGHDSDIYGNIVSH
                     CWDDGLEVEGGNRNVRVWGNYIRQCAMMIGNAPTSIGPLYIWGNVVERSQWRPDYTGG
                     SFLKMGFAGSEDWMTGHMYIFHNTIFAGDPLLPTGGFGGNRIVKHTVSRNNILHVRSP
                     DHRSISSAPQNVDNDFDYDLFNGRVPEDHEKHGIRGVPRYVAQAGFDPETKTGIFQLE
                     DTSPGAGAGIVIPNFTPPFVGKAPDVGAHQRGAPPMRFGISAAVAASSR"
     gene            complement(3749..>5346)
                     /locus_tag="PKI05_03165"
     CDS             complement(3749..>5346)
                     /locus_tag="PKI05_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M60 family metallopeptidase"
                     /protein_id="HOA51227.1"
                     /translation="LADCGIFWADGYLQRTTRMGFASSPLPGAEFHALEAAKLLGWGK
                     TPNNDVPDEVIEQAAQTVTLALSVVPQNHPLRQAMSRGKGLSSVAVIPTPQSPLRQSD
                     KAARAMLQLQVRQSLDAEANMVKAHPAAVVFPGQPPTSARTVRKSITIDPTHTGWQST
                     GLYAVAGRPITVTFDSPDVANQVFVQIGCHTDQLWHLPEWRRVPEVVKRVTVQGPRVT
                     LASPFGGLIYLGMVRARSQMGTIRCQIEGAVEAPMYVHGQTNARDWQRIRLAPAPWAE
                     LVSDRIILTLPSEYVRSLEDPAQIMEFWTAVIAACDRLAGGPARTDAQRIVCDVQISA
                     GYMHSGYPIMTHLDIAPVLVNVDRLRRNDHGGVWGLFHEIGHNYQSPDWTFEGTGEVT
                     NNLFTVYVFDHLCAVPREKTHPALATRERLERRRKYFSAGSPFEQWKNDPFLALDMYL
                     QLQEAFGWEPFEKTFAAYRTLKPEERPKSDAEKRDQWVMRFSRAVNQDISPFFQQWGI
                     PLSPEAVQSLSDLPAWQPSILSK"
BASE COUNT         1171 a         1585 c         1492 g         1098 t
ORIGIN      
        1 gcgtcacgag cagggcggtg ggttcgctgc gcagggatat gcgcgatcca caggaaaagt
       61 gggcgtctgc atgaccacca gcggtcccgg ggcgaccaac gtcatcacgg ccctggctga
      121 cgccaagctc gacagcgtgc ccattgttgc cattacgggc caggtgggaa cctctgtcat
      181 tgggacggat gctttccagg aaacgccgac ggtcgaggtc tgccggagca tcaccaaaca
      241 tcactacctc gtcacggatg tgaacgatct ggcaagggtc ttccgcgaag ccttctacat
      301 tgcccagacg gggcgacccg gaccggtgct tattgatatc cccaagaaca ttcaactggc
      361 ccaaaccgtg cctgactaca acgcccccat ggacttgccg ggttaccggc cggtggtgcg
      421 attggcaagg ccggaagaga tccgcgaaat cgcccaggtg atcaagaaat cacgccgtcc
      481 tgtggtgtat gcaggaggcg gcgtgatcac ctcaggggct tcggaggcct tgcgcgagtt
      541 tgtcgagaag acctcgattc ctgtggcgat gacagccatg gggctaggtg gttttcccgg
      601 cgatcatccc ctgtcccttg atatgctggg aatgcacggc agcatttacg ccaattacgc
      661 ggtggctgaa tgcgacctcc tgctggcctt cggcgttcgg ttcgatgatc gcgtaacggg
      721 aaaggtctcc gagtttgcta agcacgccac gatcgttcac atcgacgtgg atccgtcgga
      781 gttgaacaaa gtcaaacctg cccacatcgc cgtggtgagc gatctcaaat acgccctgga
      841 agagctgaac aagattgtgg aacccccgga catttccgag tgggtcaccc gggtcatgga
      901 gcgcaagaag gccgaaccat tctcttatcg cgaatatgag gatgccatcc tcccacaaca
      961 ggccatccac gagctttgga agctcaccaa ggaccaggat acgatcatta ccacgggggt
     1021 tggccagcat cagatgtgga cggcccagtt ttataagttc cgcaaaccac ggacgtggat
     1081 ctccagtctg ggattgggca cgatgggctt tggccttccg gccgccatcg gtgctaaggt
     1141 ggctcatcca gataaaatcg tcatcgacat cgacggggac ggtagcctgc ttatgaatat
     1201 ccaagagatg gcgactgcct attgcgaaaa gattcccgtc aaggtcctcc tcctcgacaa
     1261 tcaacacctg ggcatggtgg tgcaatggga ggatcgtttc cacgcccgca atcgtgccca
     1321 tacgtatctc ggcccgatcg accaacctga ggccgtcggc cgcggagagg gaaagatccc
     1381 ccgctatcat taccccgatt tcgtgacgat tgccaagggg ttcaactggc gggcaaggca
     1441 catccggcat aagtccgaac tcattgatgg cctcaaagag atgctggcca gcgaggaacc
     1501 gtatctcctt gatgtgctgg ttccctacca agagcacgtc ttgcccatga tccccgccgg
     1561 aatgacgatc aaggagctaa tccgcgagtg atgacccgcc ttcgcacatg gggcaccggc
     1621 agcaacaact gtttacgggg aatgtaggat cttcccgaaa gacacccgta aggggagggt
     1681 cgccgtcctc ggcgaccgga gattttcatc gtcctcagcg accgcagaat cgggagggtc
     1741 gccgtcctcg gtgacctcag gccggccacc gtgctgtcct tggcgaccaa atcctcggac
     1801 ggcggggacg ccgtccctcc cttttgttcg cccactctgc agagtgtagt tctaaaacag
     1861 gcgaccgtca ccgggatgac gcggccaccg cggcggaaat accgaaccgc atgggcggcg
     1921 caccccgctg atgtgccccc acatcaggcg ccttccccac gaaaggcgga gtaaagttcg
     1981 gaataacgat gcccgcacca gcacccggac tggtgtcctc aagttggaag atgccggttt
     2041 tcgtctcggg gtcgaaccca gcctgggcca cataccgggg cacaccgcgg ataccgtgtt
     2101 tctcatggtc ttcggggacc cgtccgttaa acaggtcata gtcgaagtcg ttgtccacat
     2161 tttgaggggc cgagctgatg ctgcggtgat ccggcgagcg aacgtgcaag atattattgc
     2221 gggaaactgt gtgcttaacg atgcgatttc ccccgaagcc gccggtgggc agcagcggat
     2281 caccggcaaa gattgtgttg tgaaagatgt acatgtgacc ggtcatccag tcctcgctgc
     2341 ctgcgaagcc catcttgagg aaagagcctc ccgtgtagtc gggcctccac tgactccttt
     2401 caaccacatt cccccagata tagagcggac cgattgaggt aggggcgttt ccgatcatca
     2461 tcgcgcattg tctgatgtaa ttcccccaaa cccgcacgtt ccggttgcca ccctcgactt
     2521 ccaggccatc gtcccagcag tggctgacga tattgccata aatatcagaa tcgtgacctg
     2581 gcgaaccgcg gtagctgcca ttgcttcctc cgcccacgat gtcgttatac atgtggtcga
     2641 ggtcggaata catttcgttg taccggatga catgatttcc ggcggtgtca aagaaggaga
     2701 tgcactgcgg accttgggga tgcgtgggat aaaccggctc gtaccacgtg ttcgtgtcgg
     2761 agaaaggatg atgcatccgg cacctttgga tgatcaccct gtgaacatcg cggttgcgac
     2821 aaagaacggc cgcgtcatag tcgaatccga agcctgtttt cggattggtc cgcccccagt
     2881 cggaaatgtc gcagtcctca atgacaatgt catgcccttt atcaattcga atggcgccaa
     2941 tgggagatcc gcgcggattg tttgtcgccc ccgcgccttt gagcacgaaa ttcctcacaa
     3001 tcacataaga ggcgtcgatg ctgatgcacg tatcgtgctt gtgctctaca tcaatgacgg
     3061 ccccttgacc gtcgtacacg cggtaggcgt ccggccggcc cgattcggta atggcgagcg
     3121 gttccttccc gccggggacg ttgatcacct caccgatggg aaactcctcc gaccacgtag
     3181 aggcgaccag ccgagccact tgatccgttc cttccagttg taactcgatc tcgtacttgg
     3241 tgcccggtgt taaatggacc aaactgcccc ggtagtcggc gagatcctga tccgtcttag
     3301 gaatcggatt gtagcgcatg ggtaggccct gctgccaagc ttcttcaccc tggcggcgaa
     3361 aacgcaccaa aacctgccga tcgggtgatc ccccgggcgg cgaccagtaa agaccaatac
     3421 agtgaaacgt ggaaatggcc acgggctgct gcgaggcatc ttccgccgaa gcgattgaaa
     3481 acagccagcc cagaacacaa ataacggtcg ccggcactgt caaaacgaga aagcttttca
     3541 tcccgtgtgc ctccccaacc aaatgccaca tctcttcacg aactgccatc cgatcttaca
     3601 cccggagaca cgacacgtag cccattatgt gccagtcaac ctccgttgaa catccttagc
     3661 gatgatgaac acgctgtcat cagatcgcgc cttttgcgaa gcgtgccact tgacgcaaca
     3721 atgttaccaa gtggatcacc gcgcatgatc acttggacag tatcgatggc tgccaggcgg
     3781 gcagatcact caacgactga acggcctcgg gcgacagggg tatcccccat tgttggaaga
     3841 aggggctgat atcctggttg acggcacgag aaaaacgcat aacccattga tcgcgcttct
     3901 cggcatcact tttcgggcgt tcttcgggtt tcagtgttcg ataggcggca aatgtctttt
     3961 caaagggttc ccagccaaac gcctcttgta gctgtaaata catatccagt gccagaaatg
     4021 gatcattttt ccactgctca aacggactac ctgccgaaaa gtattttcgc cgcctttcga
     4081 gtcgctcgcg cgtggccagc gccggatgag tcttctcgcg tggaaccgca cagaggtgat
     4141 caaaaacata cactgtaaac agattatttg tgacttcgcc tgtgccctcg aaggtccaat
     4201 ccggtgactg gtagttgtgc ccaatctcgt gaaagagtcc ccacacgccc ccgtgatcat
     4261 tccgccgtag ccgatccaca ttcaccagca caggggcaat gtccagatgc gtcatgatgg
     4321 gataaccgga atgcatatat cccgcgctga tttggacgtc gcacacaatt cgctgcgcat
     4381 cggtccgggc tggtccgccc gccagacgat cgcaggccgc aatcacggct gtccaaaact
     4441 ccatgatctg ggcggggtct tccaatgacc gcacatattc ggacggcaaa gtgagaataa
     4501 tgcgatcaga gacaagctcc gcccacggag ccggggccag ccgaatcctc tgccagtccc
     4561 tggcgttggt ctgaccgtgc acatacatcg gggcttctac tgccccctcg atctggcacc
     4621 tgatggtacc catctgcgac cgagctcgaa ccattcctaa atagatcagc ccaccgaacg
     4681 gactggccag ggtaacgcgg ggaccctgta ccgtgacccg tttaaccact tccggcaccc
     4741 gtctccactc tgggagatgc cagagctggt cggtgtgaca gccaatttgt acgaacacct
     4801 ggttggccac gtcgggagaa tcaaatgtga ccgtgatcgg tcgtcctgcc acggcgtaca
     4861 accccgtgct ttgccagccc gtgtgtgttg gatcaatcgt gatgcttttg cgaacggtac
     4921 gggcagaagt tggcggctgc ccgggaaaca ccacagcagc gggatgggcc ttcaccatat
     4981 tcgcctcggc gtcaagcgac tgccgcactt ggagctgaag catcgcccgt gccgccttgt
     5041 ccgactggcg aagtggcgac tgcggagtcg gaatgacggc aaccgaagaa agacctttcc
     5101 ccctcgacat ggcctgtctc agcggatgat tctgaggaac gaccgacaag gcaagcgtga
     5161 cagtttgggc agcctgctcg atcacctcat cgggaacgtc attgttgggt gttttgcccc
     5221 aacccaagag tttggccgct tccagggcgt gaaattctgc tcccggtaaa ggggaactcg
     5281 cgaaccccat tcgggtcgtt cgttggagat aaccatccgc ccaaaaaatg ccacagtcgg
     5341 ccaaca
//
LOCUS       DAOPTR010000105         5338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_12518_length_5338_cov_2.868825, whole genome shotgun sequence.
ACCESSION   DAOPTR010000105 DAOPTR010000000
VERSION     DAOPTR010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12518_length_5338_cov_2.868825"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..916
                     /locus_tag="PKI05_03170"
     CDS             179..916
                     /locus_tag="PKI05_03170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOA51228.1"
                     /translation="MLDWDSRHFGFAVAKANWSGEGSDLQRLINWAVDKQVTLLYLFA
                     PTSVVLPTWTTQRCVGVFVSERVEFEKAVGSNRDADFGAEVAIMELTAENCQLDSVTR
                     LGVLAGRYSRFSRDPRIPQGKAQKLFEIWAEKSVRRELADTVFGAFRADGDLIGFVTL
                     KQDGLIGRIGLLTVVPERRSMGCGRALVCATETWAAARKLTTLRVVTQADNEAACGFY
                     RRVGFHECERQVVFHLWLREPSNGTRH"
     gene            880..1848
                     /locus_tag="PKI05_03175"
     CDS             880..1848
                     /locus_tag="PKI05_03175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOA51229.1"
                     /translation="MAPRTEQRYETLNTDLSIVIPVFRGAATIASVVEGVIDCLRAAG
                     MSGEIILVDDGSPDETWQVLKDLHAEFPNIVRAIRLMRNFGQHNAIVCGLRHATGEYI
                     VTMDDDGQHPPEELPKLIRAIEESGADVVYGVPKERSHAPWRNLGSWLVVTFYQWVFR
                     TNVTPSAFRIMRRQVVDAILTYDLNYTYVDGLLAWNTDRIAQLEVEHRPRQHGRSGYN
                     LGKLLTLAMNLFTNFSILPLQIVSIMGFVVAVLGFLLGLYYILQRLLGGIAVPGYASI
                     IVAILVLGGLQMLSVGIIGEYLGRVHININRKPQYTIREILTQGEP"
     gene            2279..3073
                     /locus_tag="PKI05_03180"
     CDS             2279..3073
                     /locus_tag="PKI05_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51230.1"
                     /translation="MCLCLAGPIWSPEIGWQNFYLGKFSPNAWHNPTLLLSWPFIILH
                     FAGLLQCLNQPNFKRLCQVLFYLAVATVIKPNYTVALCGALPITLLLKSFRSPAWIRA
                     VILQLAVAVLVLLPQFAAFQAEENAVVVLAPFTIVSQRSGFIPLTFLTSVAFPLIVAI
                     TLRRGLLGNQAFQLAALLFLVAAAQCYLLVESGPGRSALHGNWYWGAHATLFLLFLVS
                     LAALWRATFGEARRDFGIYACWGVFGLHVVTGVLWLTKHFIACSYY"
     gene            3185..4570
                     /locus_tag="PKI05_03185"
     CDS             3185..4570
                     /locus_tag="PKI05_03185"
                     /inference="COORDINATES: protein motif:HMM:NF039639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HOA51231.1"
                     /translation="MGHRTFPILGLVASLAFVPILLWGQFLFPIGDDLESANWPGGWH
                     ALYQVPFTLWHTWSGCYSASFFRVLHAPLTRAGVFWTTPLLFLILHGICGTLALRLCR
                     GVRTVKTFIVALIWALAYFATMPAPAETVFWATGMLVYEPGNILALFLTIVASGSQRE
                     GRQKSLQTVLLWTLTPLIAGCHFVFALWAIAILGLRTWENGGKRADVILLIWCLFFAG
                     LVIGAPGNFQRYVFRMSDAPITFGYVVTRSIGSFVEFLIRELSLVQNWLWVTFGTIFV
                     SRHVVKENNRSTLVGVIVRVLAVLLPIFGIFVLSTMTREVPPPARTLNALHYLLMLGV
                     IVVVAPVVLKLSWVSARLHRWLDDSHVFRKAAAATLCFAVLSPNYVRCVRELVTVAPQ
                     YKQYWYEVERRVAEAKEKGLPRVEIPSDPERRPVTVFHPSFLGPDPEAWPNRDYAKFF
                     GIAQIVAVETK"
     gene            4661..>5338
                     /locus_tag="PKI05_03190"
     CDS             4661..>5338
                     /locus_tag="PKI05_03190"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA51232.1"
                     /translation="METQTGNQHRTMRICVVTPQPTPYRDPFWNAVAAQPGVELDVFY
                     CYAKGDDRPWEIDWPFQFHAEVLPGRAWLGQHNGYWNPAILKRLRAKRYDAILLGGYN
                     HFTMLAAAWYGRKRGIPYYLMSEVYLRQPRSRWKTVVKAPFLRLLLKHSAGGLPTGTL
                     AKEYLLHYGLQEDRLCFVPNAPDIDSLRKLAEPFWAKSKVEIRRSLGLRTEPLVLFVG
                     RLLALKNV"
BASE COUNT         1130 a         1286 c         1471 g         1451 t
ORIGIN      
        1 atctgccggt gacggaaagt cttgcggccc gtttggtccg cttgcccatg tattacgagt
       61 tgacacaaca cgatcaggaa agggtcgtcg tcaccctaga aaagcttttt agcgagatac
      121 tgatatagtc gcagtggtcg agaatagagt aaatgcgact gatacgatta acccgattgt
      181 gttagattgg gacagtcgtc attttggctt tgccgttgca aaggcgaact ggtcaggaga
      241 gggaagtgac ctccaacggc tgattaattg ggctgtagat aagcaggtga cgcttttata
      301 tctttttgct ccaacttcag ttgttttgcc tacctggacc acccaacgct gcgtgggtgt
      361 ctttgtgtct gaacgtgtcg agtttgaaaa agccgttggg tccaaccgcg acgccgattt
      421 tggggctgaa gtagccatca tggaattgac cgccgagaac tgccagttgg actcggtaac
      481 tcggctgggg gtgcttgcag ggcgatattc aaggtttagt cgtgacccgc gaatcccaca
      541 ggggaaagct cagaagctct ttgaaatctg ggccgagaag agcgtacgaa gagagttagc
      601 tgacaccgtg tttggggctt tccgtgctga cggggattta ataggattcg tcactctgaa
      661 gcaagacggt ctgattgggc gaatcggctt gctcacggtt gtaccagaga gacggagcat
      721 gggctgcgga cgagcattgg tttgtgccac ggaaacctgg gcagcggcac gaaagcttac
      781 cacgctacgc gtcgtcacac aagctgataa tgaagctgct tgtggtttct atcggcgagt
      841 cgggttccac gaatgcgaac gccaggtggt ttttcacctg tggctccgcg aaccgagcaa
      901 cggtacgaga cattgaacac ggatctttcc attgtcattc cggtctttcg cggtgctgcc
      961 acgattgcgt cggtcgtgga aggggtaata gattgtttgc gagccgccgg tatgtctggt
     1021 gagatcattc tggtggacga cggtagcccc gatgagacgt ggcaggtttt gaaggatttg
     1081 catgcagaat tcccgaacat tgtgcgagca atccgcctaa tgcgcaactt tggccaacac
     1141 aatgcgatcg tgtgtggtct gcgccacgcc actggagaat acatcgtgac aatggacgac
     1201 gatggacagc acccgccgga ggagctaccc aagctcataa gggccatcga ggagagcggc
     1261 gcggacgtgg tttatggggt tcctaaggaa cgtagccatg ctccgtggcg gaatttagga
     1321 tcgtggttgg tcgtgacttt ttatcaatgg gtatttcgga caaacgttac gcctagtgct
     1381 ttccgtatca tgcggcggca ggttgtggat gcgatcctta cttatgattt gaattacaca
     1441 tacgtcgatg ggcttctggc atggaatacg gaccggatag cacaactcga ggttgaacat
     1501 cggccacgac aacatgggcg atcgggatac aacctcggca agttgttgac actggcgatg
     1561 aacctgttta caaacttttc cattctgccg ctgcaaattg tttcgatcat gggctttgtg
     1621 gtagcggtgt tgggtttctt gctcggtctt tactacatcc tccagcggct tttgggagga
     1681 attgccgtcc ccgggtatgc atcaatcatt gtggccattt tagtgctggg tggactgcaa
     1741 atgttatccg tggggattat cggggaatac ctgggccgcg tgcatattaa catcaaccgc
     1801 aaaccgcaat acacaatccg ggagatattg acacaggggg aaccgtgaga tgattgggcc
     1861 aggcttaccg cacatccctc tgtcagtatc gacacttgtc ccctatctcg ccattgggcc
     1921 tattacgttg gcaatttttg cacccctata ttatgagatg gcccgtggga caattagttc
     1981 ggatattccc gctcatgcag aaattataaa aaaggtagtc gaagttggac gttggccacc
     2041 gcacccagga ttacatggtc tagcccttgt attacttggg ttccctagtt cgcttaactg
     2101 gaaaagcgcg atcatcgttc tctcagggat cacccttggg tcacttctcg tgaaacacct
     2161 tctggcggcc acctatctcg cgtcctgggc atcatttcct tccgctaacc accgagaatc
     2221 aatttcagcg tcatgcaccg acgcggggcc tgtcggatgg agactcgtcg gactgactat
     2281 gtgcctttgt ctcgccggac caatttggtc cccggagata ggatggcaaa acttctatct
     2341 gggcaagttc tcgccgaatg cctggcacaa tcccacgctt ttgttgtcgt ggccattcat
     2401 catcctgcat tttgccggtt tgctgcaatg cctgaaccaa cctaacttta aacggctttg
     2461 ccaggtcctt ttctacctgg ccgttgctac cgtcatcaag ccgaactata cggtcgcctt
     2521 gtgtggggca ctccctatta cgctccttct taagtcgttc cggtccccgg cctggattcg
     2581 ggccgtgatc ctccaactcg ctgttgccgt gttggtccta ctccctcagt tcgctgcttt
     2641 ccaggcagaa gaaaacgccg ttgtggtact cgcacccttt actatagtgt ctcaacgaag
     2701 tggatttatt ccgttgacat tcttgacttc ggttgctttt cctctaattg tcgctattac
     2761 cctgcgtcga ggtctccttg gaaatcaggc ctttcagcta gcggcattat tgtttttggt
     2821 agctgccgcg caatgttatc ttttagtaga aagcggacct ggccggagtg cgttgcacgg
     2881 caattggtac tggggcgccc atgcaacgtt gttcctcctc tttctggtgt cacttgcggc
     2941 attgtggcga gccaccttcg gcgaggcacg ccgcgatttc ggcatttacg cctgttgggg
     3001 tgttttcggt ctccacgtgg taacgggggt actgtggttg acgaagcatt ttattgcttg
     3061 tagttattat taaaacggca tctatctgca gaacaacaaa acggcgcaat cccgtagaag
     3121 tatttcataa tagcctgtta tcaaagcatt gagaccactg cattttcaaa cgtcggatct
     3181 tgacttgggt catcggactt ttcctattct gggattggta gcgagtctag ccttcgtacc
     3241 aattcttctt tggggacaat tcctttttcc catcggggac gatctggaaa gtgcgaactg
     3301 gcccggtggc tggcacgccc tgtaccaggt tccttttact ctatggcata cctggagcgg
     3361 ctgctattcg gcatcgtttt ttcgagtact gcacgctcct ttgacacgag cgggtgtttt
     3421 ctggacaaca ccgctgttgt ttttgatcct ccatggaatc tgtgggacac ttgctctgcg
     3481 actgtgccga ggggtgagaa cggtaaagac tttcattgtc gcactaattt gggcgctggc
     3541 ctattttgcg actatgcccg cgcccgccga aacggtcttt tgggcaactg ggatgctcgt
     3601 ttatgagccg ggaaatattt tggctctatt cttgacaatc gtggcaagcg gatcacagag
     3661 ggagggacgg cagaaatcac tccaaaccgt tcttctgtgg actttgacac cattgatcgc
     3721 ggggtgccac tttgtattcg ctctctgggc catcgcaatc ctgggattga gaacctggga
     3781 gaatggaggg aaacgggcgg atgtaattct cctaatttgg tgtttgttct tcgccggatt
     3841 ggttattggt gcgccaggaa actttcaaag gtatgtgttc aggatgagtg acgcgccgat
     3901 cacgttcggt tatgttgtga ctcgctcgat cggctctttc gtcgagtttt tgattcgcga
     3961 gttgagtctt gtgcaaaact ggttgtgggt aacttttggg acaatatttg tgtcgcgtca
     4021 cgttgtcaag gaaaacaatc gttcgacgct ggtaggtgtc atcgtgcgag ttttagctgt
     4081 cctgctaccg atttttggaa tcttcgtttt gtcaacgatg actcgtgaag tacctcctcc
     4141 ggcacgcacg ttgaatgcct tgcattattt actaatgctg ggagtcatcg tggtcgttgc
     4201 ccctgtggtg ctgaagctat cctgggtatc cgcgcgtctt cacaggtggc tcgatgattc
     4261 ccatgttttt cgcaaggccg cggcggcaac tctgtgtttt gcagtcttgt cgccaaatta
     4321 tgtgcggtgc gttcgtgagt tagtcacagt cgcacctcag tacaagcagt actggtatga
     4381 ggtcgaaaga cgtgttgcgg aggccaagga aaaggggctg cctcgagtcg aaataccttc
     4441 cgatccagaa aggaggcctg taacggtttt tcatccgtcc ttcctcggtc ctgatccaga
     4501 agcatggccc aacagagatt atgctaagtt tttcgggata gcccagattg tagccgtcga
     4561 aacgaaatga caagaaatcg aacgctttga cttgttgagt cgcggctatt ccagtaacgc
     4621 gcgattgtcg gaaagagcca caacgcaaaa tactgttaat atggaaacac aaacgggcaa
     4681 tcaacaccga acgatgcgaa tttgcgttgt cactcctcag ccgacaccgt accgtgaccc
     4741 attttggaat gccgtggctg cgcagcccgg ggtcgagtta gatgtttttt actgttatgc
     4801 caaaggtgat gatcggccat gggagatcga ttggccgttt cagttccacg ctgaggttct
     4861 tcccggccgt gcgtggttgg gacagcataa cggttattgg aatccagcaa tcttgaagag
     4921 gcttcgcgca aagcgttacg atgccattct cttggggggc tacaatcatt tcacgatgct
     4981 tgccgctgca tggtacggcc gaaaacgggg gataccatat tacttaatga gtgaggtcta
     5041 tctacggcaa ccacggtcgc ggtggaagac tgttgtaaag gcgccattct tgcggctgct
     5101 tcttaaacac tctgctggtg gactacccac ggggacattg gcgaaggagt atctcctgca
     5161 ttacggtctt caagaggatc ggctgtgttt cgtaccgaat gctcccgaca tcgacagcct
     5221 cagaaaatta gcggagccat tttgggcaaa aagtaaggta gaaatacgtc gcagtttggg
     5281 gttgagaacc gaacctctgg ttcttttcgt tgggaggctt ctcgcgttga agaacgtt
//
LOCUS       DAOPTR010000106         5332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_12536_length_5332_cov_3.548797, whole genome shotgun sequence.
ACCESSION   DAOPTR010000106 DAOPTR010000000
VERSION     DAOPTR010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12536_length_5332_cov_3.548797"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..872
                     /gene="rplB"
                     /locus_tag="PKI05_03195"
     CDS             15..872
                     /gene="rplB"
                     /locus_tag="PKI05_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOA51233.1"
                     /translation="MGIRAYKPTSPGRRGATVSDFAELTPGAEPEKSLLVPLKKKGGR
                     NNQGIITCRHRGGGHKRLYRIIDFKRNKDGIPARVDSIQYDPNRSARIALLKYADGEK
                     RYIIAPDGLRPGDTVMSGPGAPPNIGNALPLSEIPLGMQVHNIELIPGKGGVMCRSAG
                     CAATLVARDGDWAQLTLPSGEIRRVSARCRATIGVVSNADHEGIVLGKAGRKRWLGRR
                     PHVRGTAMNPIDHPHGGGEGRTKGGRHPVSPTGKPAKGGYTRKRGKPSNKAIVRRRRS
                     VRYGQLPIR"
     gene            924..1193
                     /gene="rpsS"
                     /locus_tag="PKI05_03200"
     CDS             924..1193
                     /gene="rpsS"
                     /locus_tag="PKI05_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOA51234.1"
                     /translation="MGRSLKKGPFVDPNVFAKVAKLNEAGKKEPIKTWARACTIVPEF
                     VGHTFLVHNGKTHVKVFVTEDMVGHRLGEFAPTRIFRGHGGRGKK"
     gene            1284..1649
                     /gene="rplV"
                     /locus_tag="PKI05_03205"
     CDS             1284..1649
                     /gene="rplV"
                     /locus_tag="PKI05_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOA51235.1"
                     /translation="MSISNMSRMYRALHRYARISPRKARLVADVVRGKQVDEALAILK
                     TMPHRAARMIEKVVSSALANSADLQDPFPRELHVIRIWVDEGPRLKRLRPLSRGMATI
                     IQRRMCHIGVILGPREYLK"
     gene            1680..2417
                     /gene="rpsC"
                     /locus_tag="PKI05_03210"
     CDS             1680..2417
                     /gene="rpsC"
                     /locus_tag="PKI05_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOA51236.1"
                     /translation="MGQKVNPIGFRTGITLDWKSRWIAPKREFADLLLEDYKIRRYIL
                     ERKEPSAEKGGRGERRAYPMISKIEIERTRDEVKVIIFTARPGVLIGSKGGKIDQLQQ
                     ELQELTGRRINLKIEEITRPELVAQLVAESIAEELERRVSFRRAMKKAMETAMAQGAR
                     GIKVQLSGRLGGAEMSRREKQIMGSVPLSTLRANIDYGFAEATIAQGQIGVQVWINLG
                     MFSEVDSHGVDAKTGQTSKKSKRAYKG"
     gene            2356..2772
                     /gene="rplP"
                     /locus_tag="PKI05_03215"
     CDS             2356..2772
                     /gene="rplP"
                     /locus_tag="PKI05_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOA51237.1"
                     /translation="MALMPKRVKHRKSQRGRIRGNATRGNRVVFGEYGLQAVQGGWIP
                     AATIEAGRIAAQQYLRSEGRLYIRIFPHKPITSIPLETRMGKGKGEPEYWAAVVKPGT
                     ILFELGGISEEAARLCFSRLAHKMPVKVRFVRRQTM"
     gene            2848..3057
                     /gene="rpmC"
                     /locus_tag="PKI05_03220"
     CDS             2848..3057
                     /gene="rpmC"
                     /locus_tag="PKI05_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOA51238.1"
                     /translation="MKARELREMSTEQLEALLRETTEALFRLRIQKQTERLASPMEPR
                     MKRRLIARIKTILNERARQEASAAK"
     gene            3125..3493
                     /gene="rpsQ"
                     /locus_tag="PKI05_03225"
     CDS             3125..3493
                     /gene="rpsQ"
                     /locus_tag="PKI05_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOA51239.1"
                     /translation="MPRRREIGIVTKANTPQLRRVEVIRLVQHPRYKKYVRHRTICYV
                     HDENNESQVGDRVEIMESRPLSRLKRWRLVRVVERGRAELLQKRRVVEEELLEASRTG
                     TEAREASPASQETQQSDQQG"
     gene            3557..3925
                     /gene="rplN"
                     /locus_tag="PKI05_03230"
     CDS             3557..3925
                     /gene="rplN"
                     /locus_tag="PKI05_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOA51240.1"
                     /translation="MIQMQTRLDVADNTGVKELMCIKVLGGSRRRYATLGDIVVCSVK
                     GVIPGSTFQKGAVVRAVIVRTKKPKRRPDGSYVRFDSNAAVIVDNDGNPRGTRIFGAV
                     ARELRERNFMKIVSLASEVV"
     gene            3925..4278
                     /gene="rplX"
                     /locus_tag="PKI05_03235"
     CDS             3925..4278
                     /gene="rplX"
                     /locus_tag="PKI05_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOA51241.1"
                     /translation="MWIRIDDTVEVISGDDRGVRGRVLRVDRKQGRVVVEGVNIVLKH
                     VRRSRQARQGGRLSKERPIDISNVMLVCPSCKQRTRVGARFLADGSKVRYCKKCDANI
                     GQISRPKSRSQTANA"
     gene            4299..4844
                     /gene="rplE"
                     /locus_tag="PKI05_03240"
     CDS             4299..4844
                     /gene="rplE"
                     /locus_tag="PKI05_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOA51242.1"
                     /translation="MEPRLLEKYRKEVIPILMEKTGRTNRLSLPRLEKIVINMGVGEA
                     KKEKKYLDEAVDALTNIAGQKAVITRARKSVAGFQVRQGMAIGCRVTLRGRRMYEFLD
                     RLISIALPRVRDFRGLNPRAFDGHGNYALGLTEQMVFPELNPDKYSRNQGMDICIVTT
                     ARNDREALELLKALGMPFRAE"
     gene            4885..5070
                     /locus_tag="PKI05_03245"
     CDS             4885..5070
                     /locus_tag="PKI05_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOA51243.1"
                     /translation="MASKSKIAKFRKKPKFSTRFRNRCRICGRPRAVYRKFGICRICF
                     RKLADNGMIPGVVKASW"
     gene            5087..>5332
                     /gene="rpsH"
                     /locus_tag="PKI05_03250"
     CDS             5087..>5332
                     /gene="rpsH"
                     /locus_tag="PKI05_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOA51244.1"
                     /translation="MMTDPIADVLTRIRNAVRVERPHVDVPTSNLKRGLADVLKREGY
                     VWDWEEIPSQPRAILRIYLKYGPNGEQVIRHIRRISKP"
BASE COUNT         1268 a         1340 c         1671 g         1053 t
ORIGIN      
        1 acgaggccag tatcatgggg attcgtgcct acaagccgac atcaccgggg agacggggag
       61 ccaccgtcag cgatttcgcg gaactgacgc ctggcgctga gccggaaaag agtctccttg
      121 ttcccctgaa aaagaaaggt ggccgaaata accaaggcat catcacctgc cgacatcgcg
      181 gtggtggcca caagaggctt tatcggatca tcgactttaa acggaacaag gatgggattc
      241 cggcgcgggt ggattccatc cagtacgatc ccaatcgcag tgcgcggatc gccttgctga
      301 agtacgccga cggcgaaaag cggtacatca ttgccccgga tgggctcagg ccgggcgata
      361 ccgtgatgag tggtcccggc gcgcccccga acataggaaa cgccctgccg ctatcggaga
      421 ttcccttggg tatgcaggtg cacaacattg aactgatacc cgggaaaggt ggcgtcatgt
      481 gtcgctccgc gggatgcgca gccacgctgg tggcccgtga cggagattgg gcccaattga
      541 cactgcccag cggcgagatt cgtcgtgtct cggctcgttg ccgagcgacg attggtgtgg
      601 tcagcaacgc cgaccatgaa gggattgtct tgggcaaggc tggccgaaag cggtggctcg
      661 gtcggcgacc acacgtccgt ggtactgcca tgaacccgat cgatcacccc cacggtggtg
      721 gtgaaggacg aaccaagggc ggacggcatc ccgtcagccc cacaggcaag cccgctaagg
      781 gcggatatac ccggaagcgc ggtaaaccat cgaataaggc gattgtccgg cggcgtcggt
      841 ccgtgcgata tggtcagctt ccgatacggt gagaaaaaca gtcaatagcc aagtgatttt
      901 ggatttcagg cggaaaaacg actatgggac gttcactgaa gaaaggtccg tttgtcgatc
      961 ccaacgtctt cgccaaggtg gctaagctca acgaggcggg aaagaaggaa ccgattaaga
     1021 cgtgggcaag ggcatgtacc atcgtgcccg agttcgtcgg gcacaccttc ctggtgcaca
     1081 acggcaagac acacgtcaag gtatttgtca cagaagatat ggtcggacac cgcctgggcg
     1141 agttcgcgcc gacgcggatt ttccgcggcc acggcggacg tgggaagaag tagttttgga
     1201 ggtataggcc aagaggcgat ttggcaagct ccgaaggttc cagtagatag cagcctcgga
     1261 gcaaaaagga caaggtcgga atcatgtcca tttccaatat gtcgcgaatg tatcgagcct
     1321 tacatcggta cgcccgaatt agcccgcgca aggcgaggct ggtcgctgat gttgtgcgtg
     1381 gcaaacaggt cgatgaggcc cttgctatat tgaagaccat gccccatcgc gctgcgcgca
     1441 tgattgaaaa ggtggtgagc agcgccttgg ccaattcggc tgacctgcag gatccgttcc
     1501 cgcgagagtt acatgtcatc cgcatttggg tggatgaggg tccacgattg aagcgacttc
     1561 gtccgctgtc ccgcggaatg gccacgatca ttcaacggcg gatgtgccat atcggcgtga
     1621 ttctcggacc tcgtgaatac cttaagtaat ttgggtggga ttattgagga tacctggtta
     1681 tgggacaaaa ggtaaacccc atcggttttc gcactggtat cacgctcgac tggaagagcc
     1741 ggtggatcgc gccgaagcgc gagttcgctg atctcttgct cgaagactat aagatccgtc
     1801 gttacatttt ggagcggaag gagccatccg cggaaaaagg gggacgcggc gaacgacgtg
     1861 cctatcccat gatctcgaaa atcgagatcg agcggacgcg cgatgaagtg aaggtgatca
     1921 tattcacggc ccgtcctggt gtgctaatcg gatcaaaagg cgggaagatc gaccagctcc
     1981 agcaggagct ccaggagctg accgggcggc ggatcaattt gaagatcgaa gagatcacac
     2041 gtcccgagct ggtggcccaa ctggtggccg aaagcatcgc ggaagaactg gagcgccgcg
     2101 ttagctttcg acgtgccatg aagaaggcca tggaaacagc catggcacaa ggtgcccgag
     2161 gaattaaggt ccagctctcc gggcggcttg gcggggccga aatgtcgcgg cgtgagaaac
     2221 aaattatggg atccgtccca ctctccacgc ttcgggcaaa tatcgattac ggctttgccg
     2281 aagcgacaat tgctcaggga cagattggcg tccaggtgtg gatcaatttg ggaatgttta
     2341 gcgaggttga tagccatggc gttgatgcca aaacgggtca aacatcgaaa aagtcaaaga
     2401 gggcgtataa ggggtaacgc gacacggggt aaccgcgtcg tgttcggcga atacggcctc
     2461 caggctgttc agggtggatg gatccctgct gccacgattg aagccggacg tattgccgcc
     2521 cagcaatacc tccgctcgga aggtcggctg tacatacgaa tcttccccca caagccgatt
     2581 acctcaatcc ccttggaaac acgcatgggc aagggaaagg gggaacccga atactgggca
     2641 gccgtggtta agcccgggac tatcctattt gaactcggcg gtatttccga ggaggccgct
     2701 cggttgtgtt tttcgcggtt ggcccacaag atgccggtga aggtccgatt tgtccggcgc
     2761 cagaccatgt aatggcttgg ccagtgggat gaaaccaaca ggagataggg accgtttaag
     2821 tcgggtcaag ggtgaaaggt aacagacatg aaagcccgcg aacttcggga gatgagcacc
     2881 gaacaactcg aagccctgct ccgcgagacc accgaggcct tgtttcgttt gcggattcaa
     2941 aagcaaacgg agcgcttggc gtctccgatg gaaccgcgga tgaagcggcg cttgattgcc
     3001 cggatcaaga ccattttgaa cgagcgagcc cgacaagagg catccgcggc aaagtaatct
     3061 ggacaacacc ggaggcgctg aagaatcgtc cggcagggaa aacatttgcg aggtgaaaaa
     3121 caatatgcca aggcgacgtg aaatcggcat cgtaacaaag gcgaatacac cccagttgcg
     3181 tcgggtggaa gtcatccgac ttgttcagca tcctcgctat aaaaagtacg tgcgacatcg
     3241 tacgatttgc tacgttcatg acgagaacaa cgagtcgcaa gtgggtgacc gagtcgagat
     3301 tatggagtcg agacccctat cgcggctcaa acgctggcgg ttggtacgtg tcgtggagcg
     3361 cgggcgtgcc gaattgctcc aaaagcgacg tgtggttgag gaggaattgc tggaggccag
     3421 taggaccggg accgaagcca gggaggcttc gccggcttca caggaaacac aacagtcgga
     3481 tcagcagggg taatgttgcc gacttgttcg cagcattcga tggtcggcag gaattgcgga
     3541 aacaagggtg atcgccatga tccagatgca aaccaggctc gatgtcgccg acaacacagg
     3601 cgtcaaggag ctgatgtgta ttaaggtcct cggcgggagc cgccgacgtt atgcaaccct
     3661 cggggatatt gttgtctgca gtgtgaaggg cgtgatcccc ggtagcacct ttcaaaaagg
     3721 ggctgtcgtg cgggccgtga tcgttaggac gaagaagccg aaacggcggc ccgatgggag
     3781 ttacgtccgt tttgacagca atgccgccgt gatcgtcgat aacgacggca atcctcgggg
     3841 aacacgtatt tttggtgcgg ttgctcggga attgagagaa cggaatttca tgaaaattgt
     3901 tagcctcgca agcgaggtgg tctgatgtgg atacgaatcg atgacactgt ggaagtgatt
     3961 agtggggacg accggggtgt tcgtggccgc gtcttgcggg tggatcggaa acagggccgc
     4021 gtcgttgtgg aaggcgtgaa catcgtcctg aaacacgtgc ggcgaagccg gcaggcgcgc
     4081 cagggcggcc ggctgtcgaa ggagcggcct atcgacattt ccaacgtcat gttggtctgc
     4141 ccttcgtgca agcagcggac ccgtgttggg gcacgcttcc tcgccgacgg ctccaaggtt
     4201 cgttattgca agaaatgtga tgcgaacatc ggacagattt ctaggccgaa gtcgcggtca
     4261 caaactgcga atgcctgata tcgggggata agctgaatat ggaaccgcgt cttttggaaa
     4321 aataccgaaa agaggtcatc ccgatcctga tggaaaagac gggccggacc aaccggttgt
     4381 ctctgccccg cctggaaaag attgtgatca atatgggggt cggtgaggcc aagaaagaaa
     4441 agaagtacct cgatgaggcc gtggatgccc ttaccaatat cgcgggccaa aaggccgtga
     4501 tcacgcgggc gaggaaatcc gtggccgggt tccaggtacg ccaggggatg gcgatcggtt
     4561 gtagagtcac cttgcgtggt cggcggatgt atgagttcct cgaccgtttg atctccatcg
     4621 ctctcccccg tgtccgcgac tttcgcggat taaaccctcg ggctttcgac ggacacggca
     4681 attatgctct gggcctgacc gaacaaatgg tcttccctga actgaacccc gacaagtatt
     4741 cgcggaacca gggcatggat atctgcatcg tcaccaccgc ccggaacgat cgcgaggccc
     4801 tggagttgct caaggcattg gggatgccct tccgtgcgga atgatgagac aagaggaaaa
     4861 cctgggtttt taggatagcg gccaatggcg agtaaatcca agattgcaaa gtttcgtaaa
     4921 aagccaaagt tctcgacgcg atttcggaac cgatgccgta tttgtggtcg gccacgcgcg
     4981 gtttaccgaa agtttggtat ctgccggatt tgcttccgca aattggccga caacgggatg
     5041 attccaggcg tagtcaaggc gagttggtag agggtaatac acgaccatga tgaccgaccc
     5101 gatcgccgat gtgctgactc ggattcgtaa tgcggtgcgt gttgaacgac cgcacgtgga
     5161 tgttcccacg tcgaacctga aacgaggtct ggccgatgtg ctgaagcgcg aaggctatgt
     5221 ttgggattgg gaagagattc caagtcagcc tcgagctatt ttgcgaattt acttgaaata
     5281 tggacctaac ggcgaacagg tcatccgcca tatccgacgg attagcaagc cc
//
LOCUS       DAOPTR010000107         5327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_12545_length_5327_cov_4.317716, whole genome shotgun sequence.
ACCESSION   DAOPTR010000107 DAOPTR010000000
VERSION     DAOPTR010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12545_length_5327_cov_4.317716"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PKI05_03255"
     CDS             complement(<1..452)
                     /locus_tag="PKI05_03255"
                     /inference="COORDINATES: protein motif:HMM:NF019997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC family protein"
                     /protein_id="HOA51245.1"
                     /translation="MTTNQANQNVYQKITDQIVAAMEAGAHRFEMPWHTRNPRPVNIV
                     SGKPYRGVNTLALWVAEQMNGYASGTWGTYKQWNSIGAQVRKGERAATVVFFKDLERP
                     MDEDENEETKSRRSFVATASYVFNADQVDGYTASEVTPPVDKTERLKTV"
     gene            1625..3130
                     /locus_tag="PKI05_03260"
     CDS             1625..3130
                     /locus_tag="PKI05_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51246.1"
                     /translation="MLPTPEPLLTGLKEAELYHHVQDRGFFTLLWADPVQADRAARVA
                     VERQQLKLELAALESASPVPPLLEPPGKTVQEIKAQLERLPRLTPKIQRAYRLGDMPK
                     VIEALDQERDTWISQAEFVKPNRRVVYLLRLNLCFVDLDTYKTHWAGVLPESLSNRVQ
                     GYCLDEGIPAPSLILYSGRGLQVKWLLERPLPRAALPRWNAVQKQLVASLALFGADPG
                     ARDASRLLRLVDTVNTRSGERVRVLWVNDREGEVKHYNFEYLAETILPMARETVREER
                     KAKEDRRSRLIVIPGGKTGNLRTFSGRQLAWDRLEDLRTLAKLRGWMEQGVPHGYRSK
                     YVHWCLNFLLLSGAVHSSQLFQEAQALVREVCPDFTKEVRSILSTLYRKAQAYEAGEK
                     VEFGGREYPPLYTPRNSTLLELFGIEDAETPHLKTIISAAEAAERDRRRHRKAMDRAT
                     YLETAEQRRAEARLRRARGESLRQIAMAMGVSHEGVRKMLKDEGVNSPPYY"
     gene            complement(3381..4370)
                     /locus_tag="PKI05_03265"
     CDS             complement(3381..4370)
                     /locus_tag="PKI05_03265"
                     /inference="COORDINATES: protein motif:HMM:NF016921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HOA51247.1"
                     /translation="MGNQLIASVLYWRERINERWQDSVQAIIDVGRLLLSAKAALEHG
                     QFQSLFEGDDRLPFGWNTANKLMAIARDRRLTNSEHVQNLPNSWGTLYELTKLDNETF
                     ARALDDGSIHAEMERREVLGLRDERERKTLGTQPLFGTCTTRELEVLIARGMKFGTVY
                     ADPPWLYENQGTRAATSNHYDGMTVEEIAALPIAKLAAENAHLHLWTTNAFLFDCKAI
                     IEAWGFEYKSCFVWTKPQLGIGNYWRLAHEFMLLGVRGSCTFLDRGQISWMHEPRGQH
                     SAKPERIRDLIEKVSPGPRLELFARRITQGWVCWGNEIERSLFDQQEAVKELG"
     gene            4830..5231
                     /locus_tag="PKI05_03270"
     CDS             4830..5231
                     /locus_tag="PKI05_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51248.1"
                     /translation="MIKTKEDRLGRFGFNFRSGGTHTARTMMLEDLHLLLSSVPSLSN
                     LPSVGAAKGDYVKAIMEDNCLHKRSMRTRALTTRHLVELYALDSSVTLFRALLYFWKR
                     DPDSQALLALLCAYCRDSILRISAPFILNLP"
BASE COUNT         1145 a         1421 c         1558 g         1203 t
ORIGIN      
        1 acggttttaa gcctctcggt cttatcgacc gggggagtaa cctcggaagc ggtgtagccg
       61 tccacctggt cggcgttaaa aacataactg gcggttgcta caaaggaacg ccgggacttc
      121 gtttcctcgt tctcgtcctc gtccatcggg cgttccaggt ccttgaagaa aaccacggtg
      181 gcggcgcgtt cccccttgcg aacctgagcg ccgatagaat tccactgttt gtaggtgccc
      241 catgtcccgc tggcgtagcc gttcatctgc tcggctaccc aaagcgccaa ggtgttcacc
      301 ccccggtagg gttttcccga aacgatattg accgggcggg gattgcgggt gtgccaaggc
      361 atctcgaaac gatgcgcgcc cgcttccatt gcggccacga tttggtcggt aatcttttga
      421 taaacgttct ggtttgcctg attggttgtc ataaaagaat ctccttttca ttttcggtcc
      481 gccccatgcg gccgtttcag gcttgaaaat ggcaggcgta taaggggctt ttgcactgca
      541 aagccgaaac gaagtggagg atggtttgcg aactgttttt tggagcttta gcgtccgcga
      601 ggaatggtct ttgctgcatg gcaaagacca ggggaaaaaa cagagaagca aatccattgc
      661 aaaggacccg gcgactgcca caagcctgaa atgaaatggc cgcttttggg gcgaaccagg
      721 gaaaagggga gattcgtgat aacccagcaa accagacctt gaaaccaaga ttaccgcctg
      781 gccgcgattc ttgcgggcgt gcgaacaata gccttggcca ctgcttcccg cccggttcct
      841 gaaagcacaa gggaaaaccc gcaagggggg tgaagtgctc ggcttgggta gccgatcacg
      901 gcttgacctg ccagcggggt ttaaacatgg ggcacaaaga cagtggtatg ccggtgcagt
      961 cgctgggggg ataaagccgc gatccacacg gttttgaagg acggggaatg cccggttgta
     1021 acgaggggaa acggtccggc gtctgtggca accgcgactt taagttttgc cggtccaggg
     1081 gggtcggcct gcttccggtg gctccccgat aagaccgcaa gaatcaggag gatctgcttg
     1141 gcgataccga agaggtctcg accgatctcc tagtggggtt ggcccatgag aatagggcgg
     1201 atacggagta tcaggatgat atgaccttac tggaaaacct gccggtcgtg ctgcggggag
     1261 tctgtgacta cgtgctgtag gggggagcca aggtcctgga tgtggcctat gcgctggctt
     1321 ccctcattga tgctatcgaa accgggcaac cgccgttacc acggggaatg tggcattgag
     1381 tgttgttgaa gtgaacgcgg caaaaagtgg aagatagcct ttagggtcac tatcctgtag
     1441 cgagtatctc agcaagaaaa aggacaaaaa atcatgggcg caaaaacgcc agcgccccgt
     1501 aacccgtatg ctttggccgg cggaaggtta cgaggcactg taccgagttt gcttaaagcg
     1561 tggggcgtcg gcacttttaa tagtagccag tccccggcac ttgtacaagg gcgggggact
     1621 aaacatgctc cctacccctg aaccgctgct cacgggtctc aaagaggccg aactctatca
     1681 ccacgttcaa gaccggggat tcttcaccct cctctgggcc gatcccgtcc aagcggatcg
     1741 ggcggcgcgg gtggccgtcg agcgccagca actgaagctg gaactggccg ctctggaaag
     1801 cgccagtccg gtgcctcccc tgctggaacc tccgggcaag accgtccagg aaatcaaagc
     1861 gcaactggag cgtctcccgc gtctcacccc gaagatccag cgggcttacc gcctgggcga
     1921 tatgcccaag gtgatagagg ccctcgacca ggagcgggat acctggattt cccaagcgga
     1981 gtttgtgaaa cccaatcgcc gggtggtcta cctgctgcgg ctcaacctct gcttcgtaga
     2041 ccttgatacc tacaaaaccc actgggccgg cgtcctgccc gaatcgctgt ctaaccgcgt
     2101 ccagggctac tgcctggatg aaggtatccc ggctccctcc ctcattctct atagcgggcg
     2161 tggcctgcaa gtgaagtggc tcctggaacg gcccctgcca cgggcggccc ttccccgctg
     2221 gaacgccgtg caaaagcaac tggtcgccag tctcgctctc ttcggggccg atcccggcgc
     2281 gcgggacgcc agccgcctgc tgcggctcgt ggatacggtc aacacccgct ccggtgagcg
     2341 ggtgcgcgtc ctctgggtca acgaccggga aggggaagtg aagcattaca acttcgagta
     2401 tctggccgaa accatcctgc cgatggcgcg ggaaaccgtc cgggaagagc gcaaggcgaa
     2461 agaagatcgt cgctcgcgcc tgatcgtgat tccgggtggc aagaccggga atctccgtac
     2521 cttctccggg cggcaactgg cctgggatcg cctggaagat ttgcggaccc tcgccaagct
     2581 gcggggctgg atggaacagg gcgtccccca cggctaccgt tccaaatatg tccactggtg
     2641 tctgaacttc ctgctcctct ccggcgcggt gcattcgagc cagcttttcc aggaagccca
     2701 agccctggtt cgggaagtct gtcccgactt taccaaagaa gtccgttcca tcctgtcaac
     2761 gctctaccgc aaggcgcaag cctatgaagc gggggagaag gtggaattcg gcggtcggga
     2821 ataccccccg ctctataccc cacggaatag cacgctcctg gaactgttcg gcattgagga
     2881 tgcggaaacc ccgcacctga aaaccatcat ctcggcggcg gaagcggcgg aacgggatcg
     2941 ccggcgtcac cgtaaggcga tggatcgggc aacctatctg gaaaccgccg aacagcggcg
     3001 ggctgaagcg cggctcaggc gggccagagg ggaatcctta cggcagatcg caatggccat
     3061 gggggtaagc catgaagggg ttcgcaagat gctcaaggac gagggtgtca acagtcctcc
     3121 gtattactag tggcgtagcc ttcgggtttt tgacttccgt gttgctgtgc gtcgtggtgg
     3181 tctgatcttt ttatcgttcc ggtgtgcttc cccgaagtgg agccgccaag ccatggatgg
     3241 cgatccgttc ccgcccatgg aggggcggga acgggggtgg ccccaacaag gatgttggct
     3301 tgaccacgct ttacagggat gtttttctca ctgaacgctg tttaggagga gggggggcgc
     3361 gggggggagt ttgccgatag ctaccctagc tccttgaccg cttcctgctg gtcaaacagg
     3421 gaacgctcga tctcattccc ccagcatacc caaccctggg tgatgcggcg ggcgaacagt
     3481 tctaaccttg gccctgggct gactttctct atgaggtcgc gtatccgttc cggcttggcg
     3541 ctgtgctggc ctcggggctc gtgcatccaa ctgatttggc cgcgatccag aaaggtgcaa
     3601 ctgcccctga ctcccagcaa cataaactcg tgcgcgagtc gccagtagtt gccgataccc
     3661 agttggggtt ttgtccagac aaagcagctt ttgtattcga acccccatgc ttcgatgatg
     3721 gccttgcagt caaagagaaa ggcgttggtc gtccaaagat ggaggtgggc gttctcggcg
     3781 gctagcttgg cgatgggcaa ggcggcgatc tcctcgaccg tcatgccgtc atagtggttg
     3841 ctggttgcgg cccgcgtgcc ctggttctca tacaaccacg gcggatcggc gtagaccgtg
     3901 ccgaacttca tcccacgggc aatcaaaacc tccaattctc gggtcgtgca ggtgccaaag
     3961 agaggctggg tgccaagggt tttgcgttcc cgttcgtccc gtaatcccaa aacttcccgc
     4021 cgctccatct cggcatgaat cgacccgtca tccaaggcgc gggcaaaggt ttcgttatcg
     4081 agtttggtga gttcgtagag cgtgccccaa gaattcggta aattctgaac gtgttcagaa
     4141 tttgtcagtc gccgatctcg ggcgatagcc atgagcttgt tggccgtgtt ccagccaaag
     4201 ggcaagcggt cgtcaccctc aaacaagctc tgaaactgtc cgtgctctag tgccgccttg
     4261 gcactaagca acaagcgccc cacgtcaatg atggcctgca cgctatcctg ccaacgttcg
     4321 ttgatgcgct ctcgccagta gagcacgctg gcaatcaact ggttacccat gaaccttctc
     4381 tcccgcccat gcgatctcaa gcggcgatga tagcggcttt tgccgtgggg aacgggtggt
     4441 atcctggaga ggcaagggac ctcttgtgaa gatgtgacat ttttcctgaa gaattggggg
     4501 ttctggtcca tgagtggcgg caaaaacggc gtgccctgtg aggcgctttg ccagtagatg
     4561 gagtataaac ctttctaagg atgggataat ttgctaggtt tccaggggta acccttttaa
     4621 ggtgttcata taacaatttg atatttatga acttgttttt gctgcttgac acaattcttg
     4681 acgaaatttt cccgtaaccc gttgattcta cgttgtcacc taaaaaggac tggtttccag
     4741 ggggtttatc ctgtctttag cttgtggagg gtaaactacc gaagtgctgg tctacccctc
     4801 ttgatggagg cagggagagt gtgctcagaa tgattaaaac taaagaagat agattagggc
     4861 gttttgggtt caactttaga tctggaggaa cacatacggc tcgaaccatg atgctagagg
     4921 atctgcatct tctgctttca tctgtgccaa gtctttcaaa tttaccaagc gtaggtgctg
     4981 ctaaaggtga ttatgtcaaa gctatcatgg aggataattg tttacataag cgttcaatga
     5041 ggacaagagc ccttactact agacaccttg ttgagcttta tgctcttgat tcttccgtaa
     5101 cgctttttcg agcactttta tatttttgga agcgagatcc tgatagccaa gccttgcttg
     5161 ctctactatg cgcctattgt agagattcta tcctgcgaat aagtgctcct ttcattttga
     5221 atttacctta aaatacggtt gtaagtcgga aaattttgta agagttcata gatgagaaat
     5281 atacaagtcg atttagtaag gcgacattga agtcaaaagc taaaaat
//
LOCUS       DAOPTR010000108         5323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_12561_length_5323_cov_3.136864, whole genome shotgun sequence.
ACCESSION   DAOPTR010000108 DAOPTR010000000
VERSION     DAOPTR010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12561_length_5323_cov_3.136864"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PKI05_03275"
     CDS             <1..325
                     /locus_tag="PKI05_03275"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOA51249.1"
                     /translation="VKHGDAIDSHQGRVKLSAWLSEDDGKTWQGGLVLDDRPGVSYPD
                     GFQAPDGTIYISYDRNRATDGEILLARFTEADILAKKLVGPKSKLKILISKPLAPKKS
                     ASKVP"
     gene            complement(363..3587)
                     /locus_tag="PKI05_03280"
     CDS             complement(363..3587)
                     /locus_tag="PKI05_03280"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOA51250.1"
                     /translation="MKGSWIFILAMVLPSSLWASDDLVLVENGQPRAEIIIADRPARM
                     ARMAAHELRETIEKISGARLPIVTAPTGKAVKVFVGASTHNPITADGLRYGAYRIASG
                     PDWLALIGDDTDFEPTEPFARSNSDIPRAQAEWEKIVGAPYGMPNAGLYKHRLKLPGD
                     IGKPDGAVTEKNEAFEVWGLDERGSYNAVCDLLRRLGVRWYMPGELGEVRPSLKTVKV
                     PRINETVWPDFSLRQMNFRFGVVGAETARWAMRLGLRNDERLQIAHGMSTMTNRQAVF
                     DAHPEWFALYGGRRDFRPGDSKCQLCYSNQELFQETVRWARAMLDTYDFETVSIMPPD
                     GYVAICQCEQCRGKDTPERGERGLLSDYVWDFVNRVAREIAKTHPRAKVLNCAYGAYT
                     LPPLKIEKLEPNVVVCIVGGRRPFNRGGPKGEGEAAPEALRAAWAKKTDNPLLIFENY
                     PFTSRGWYLPAFDARTLGETVNATKGLSRGEDVWLSIGQDFHQKDIGFNHFLVYFTAR
                     AYWGGKEYDPDAELREYCRLFYGPAETEMLTFFDYCAANWRDMENDRAKIEQALALFE
                     KAKAKAETASDSVYRRRLALIDDFLKGVRMKSRQLGQKRGPLPVVRLVGDASGIVIDG
                     KLDDPYWQNCPSAATGRLRELQTGGAPVFGTTFKVGWQGNSVIFGIRCNESTGDVPLP
                     AGEFLRQPHPENRPISASARDDDPATWYGDAVEILIATESHSYYQIAITPAGHVADLD
                     RALPRDQWFAWDSKAEVATNIADDHWTVEIRLPVTEDENDPLHQILGRKPTQSLPWFV
                     NICRQCIRPSGAEYSAFSPTGTASFHEPLKFAYFYDGRSHQFDFDPEVTDFALQFRAA
                     TEQRKAEPFLALAAGRVTDFQKSAALEQAVRYDIAAAPTVIEKIPIEAVKKAAHMRYL
                     LAISRPGEVIARYAEEDFMSWPFWKRGDGYHARGRAYFMTKSGEKAERDLLAALQWTS
                     EPRTRDAILLLLAQNREQNLHNDEAALAAYRAIIDSRQKVGGADEYAALQGAARILSR
                     RGFFDEALRILNRAEPEKLQGTWRERIMKSISEIERAKETSTRP"
     gene            complement(3674..3949)
                     /locus_tag="PKI05_03285"
     CDS             complement(3674..3949)
                     /locus_tag="PKI05_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51251.1"
                     /translation="MAILSPIVISQKGIALVYTQFGGRKAFAAKSGFAQSQLEVGARS
                     GTVELPICRGVPFAVRTSILGNVWWVGIPEGIPAGRGSNDQEKTPLT"
     gene            4129..5169
                     /locus_tag="PKI05_03290"
     CDS             4129..5169
                     /locus_tag="PKI05_03290"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOA51252.1"
                     /translation="MIWGLSFLLVVLVIWATRRTLANALAEFNRVRQEGGNVVISPGY
                     TFLAGLVYFLGFLPAAWFWYRILNWLGQEVTFFRALRAYCIGQLGKYVPGKAWVVILR
                     TSLVQGEQVQPAMAAAGVFLETLTMMSVGGVLAVLYLIINLRGNPQLFWLAVGLFVIV
                     TLPTLPPFFRPILRFIARKGKREEVVVAIEKLGFARLAQGWLAMVLLWTAYGLSLSLT
                     LQSLGMKGAMMFSQLPNMVAAVSLATVAGFLVLFLPGGLGAREFVLIAVLVPALGDQV
                     VCSSGISSVELLAVIGAGLLRVIWLITEIVVAGVFWLTLVIGTFFRLPGVTGRLQKDA
                     PSPEPCGIEAQK"
     gene            5166..>5323
                     /locus_tag="PKI05_03295"
     CDS             5166..>5323
                     /locus_tag="PKI05_03295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA51253.1"
                     /translation="MTRGLSVVIPVLNESPSLRQLYEEIRQVAETHHYDYEIIFVDDG
                     STDDSWQV"
BASE COUNT         1134 a         1519 c         1413 g         1257 t
ORIGIN      
        1 ggtgaagcat ggtgatgcaa ttgattccca ccaaggccgc gtgaaactca gtgcatggct
       61 ttccgaagat gacggcaaga cttggcaagg tgggctggtt ttagatgatc gaccgggcgt
      121 ttcctatccc gatggttttc aggcaccgga tgggaccatc tacatttctt atgatcgcaa
      181 ccgggccacc gacggtgaaa tcctgcttgc ccgatttacc gaggccgata tcctagccaa
      241 aaagctcgtc ggtcctaagt ccaagctaaa aatcctcatt agtaagccgc ttgccccaaa
      301 gaaatcggca tcaaaggttc cctaggaagc caagccgaca gctcggaacg ggttccagcc
      361 gatcatggtc gggtgctcgt ctctttggcg cgctcgattt cagagatgga cttcatgatt
      421 ctctccctcc acgttccttg aagtttttcc ggctctgcgc gattgagaat cctcagggcc
      481 tcatcgaaaa atccccgacg gctcagaata cgcgcggcgc cttgcaaggc agcgtactcg
      541 tccgcaccgc ccactttttg ccggctgtcg atgatcgcgc gataagcggc caaggcggcc
      601 tcgtcattgt gaaggttttg ctcacggttt tgtgccaaca gcaacaaaat ggcatcgcgg
      661 gtccgcggct cactggtcca ttggagggcg gcgagcaagt cacgctctgc cttctcacca
      721 ctcttcgtca tgaaataggc ccgaccgcgg gcatgataac cgtcccctcg tttccagaat
      781 ggccaactca tgaaatcttc ttcggcatag cgggcaatca cctcacctgg tcgcgatatt
      841 gccagcaaat accgcatgtg tgccgctttc ttgacagctt caatcggaat cttctcgatt
      901 accgtcggtg cagccgcgat gtcgtaacga accgcctgtt ccagcgccgc ggacttttga
      961 aagtcggtga cccgtcctgc cgcaagtgcc aagaaaggtt cggcttttcg ctgctctgtg
     1021 gccgcacgaa attgcaaggc aaagtcggtc acctccggat cgaagtcaaa ttgatgcgac
     1081 cggccatcgt agaaatacgc aaacttgagt ggttcatgaa agctcgccgt acccgtcggt
     1141 gaaaaggccg agtattcggc tccactgggg cgaatacatt ggcggcaaat attgacgaac
     1201 cacggtaggc tctgtgttgg cttgcggccg agaatctgat ggagcgggtc attttcgtcc
     1261 tccgtgaccg gcaggcgaat ttcaaccgtc cagtggtcgt cggcgatgtt tgtggcaacc
     1321 tccgctttcg aatcccaagc gaaccactga tcccttggca acgcgcggtc gagatcggcc
     1381 acgtgccccg ccggagtgat ggcaatctga taataagagt gggactcggt ggcaatgagg
     1441 atttccacag cgtcaccata ccaggtagcc gggtcatcat cgcgtgcgga agcggagatc
     1501 ggcctgtttt ctggatgcgg ttggcggagg aattcaccgg ccggcaacgg aacgtcaccg
     1561 gtgctttcat tacaacggat gccaaaaatg acgctgtttc cctgccagcc cactttgaag
     1621 gtggtcccaa agactggcgc accaccggtc tggagttcac ggagccgacc cgtcgccgcg
     1681 ctcgggcaat tctgccaata gggatcgtcc aatttgccat caatgacgat gccggaggca
     1741 tcgccaacaa gtcgcaccac ggggagcggt ccccgttttt ggccgagctg ccgtgatttc
     1801 atccgcacac ccttcaaaaa atcgtcaatg agggccaaac gtcggcgata gacgctgtca
     1861 gaagccgttt ccgccttggc cttcgctttc tcgaatagcg ccagggcttg ttcaatcttc
     1921 gctcgatcgt tttccatatc ccgccagttc gcagcgcaat agtcgaaaaa agtgagcatc
     1981 tccgtttcgg ccgggccgta aaagaggcgg caatattcac gtaattcagc atcgggatcg
     2041 tattcttttc caccccaata ggcacgagcc gtaaaataga caaggaaatg gttaaacccg
     2101 atgtccttct ggtggaagtc ttgcccaata ctcaaccaga catcctcacc ccgggaaaga
     2161 cctttcgtgg cgttcacagt ttcgcctaaa gtccgggcgt cgaaggcagg caagtaccag
     2221 ccacgagatg tgaaaggata gttctcaaaa atcagcaagg gattgtcagt tttcttcgcc
     2281 caagctgcac gaagtgcctc aggagcggct tcaccttcgc cctttggtcc accccgattg
     2341 aacggtcgtc gccccccaac aatacacacc acaacgttcg gttccaattt ctcgatcttg
     2401 agcgggggca gcgtgtaagc cccatacgcg cagtttagga cctttgctcg tggatgcgtt
     2461 ttggcaattt cgcgcgccac acgattgaca aagtcccaca cataatcgct aagtaacccc
     2521 cgttcgcccc gctcgggcgt atctttgccg cgacactgtt cgcactggca aatcgccaca
     2581 taaccgtccg gcggcataat agaaaccgtt tcaaagtcgt acgtgtcaag catcgctcgc
     2641 gcccagcgga ctgtctcctg gaacagttct tgattggaat aacaaagctg gcacttggaa
     2701 tcgcccggcc taaagtcacg tcttcctcca tataaggcaa accactcggg atgggcatcg
     2761 aagacggctt ggcggttggt cattgtggac atgccgtggg caatttggag gcgttcatca
     2821 tttcgtagcc caagccgcat tgcccagcgg gctgtctccg cacccaccac gccaaaacgg
     2881 aagttcattt gccgcagcga gaaatcaggc caaaccgtct cgttaatcct cggcaccttg
     2941 accgttttca acgagggtcg cacttcgcct agctcgcccg gcatatacca cctgacccct
     3001 agcctccgca aaaggtcaca aacggcgttg taactccccc gctcatcgag gccccaaacc
     3061 tcgaacgcct cattcttttc cgtcaccgcc ccgtcgggtt tgccaatgtc tccaggcaat
     3121 ttaagccggt gcttgtaaag cccagcattg ggcattccat acggcgcgcc gacaatcttt
     3181 tcccattccg cctgtgcacg cggaatatca ctgttgctgc gggcaaaggg ctcggtaggc
     3241 tcaaaatcgg tatcatcgcc aataagggcc agccaatccg gccccgaagc gatgcgataa
     3301 gcaccatagc gcaacccatc cgctgttatt ggattatgtg tactcgcccc cacgaaaact
     3361 ttgactgctt tgcccgtggg cgcggtcaca atcggcaacc gggctccgct gatcttttcg
     3421 atggtctcgc gtagttcgtg ggccgccata cgggccattc gtgcgggccg gtccgcgatt
     3481 ataatctccg cccgcggttg tccgttctcg accaacacca ggtcatcact tgcccataga
     3541 gacgatggca aaaccatcgc caaaatgaat atccaagagc ccttcatatg ttgtcgctcc
     3601 caataaacat gcactcatcg gccaagccta acgccatgtt agctggcgtc cggggacatt
     3661 tccaccgacc ttttcatgtc aaaggggttt tctcctggtc attactccct cttcctgctg
     3721 ggatgccttc tggaatcccg acccaccaaa cgttccctaa aattgatgta cgcaccgcga
     3781 acggaacacc gcgacaaatg ggcagctcga ctgttccgct ccgagcgccc acttccaact
     3841 gactttgagc gaaaccactc tttgccgcaa acgcctttcg tccgccaaac tgggtgtaca
     3901 ccaaggcgat gcccttttgg gaaatgacga tgggcgataa gatagccaca aggtcgttcc
     3961 gtcggttcct tttccgaaag agagggaaaa tagagaagct acaatcgagc gccgtggtac
     4021 cacaggcctg atagataaca ttctcgcgtg gggctgcctg cctacggtaa cttcggagag
     4081 acgatcttgc cagtcgttgc actaaaacgg aaaacatgga aatcttgggt gatctggggg
     4141 ttatcattcc tcttggtcgt cctcgtcatt tgggcaactc gcaggacatt ggcgaatgcc
     4201 ttggccgaat ttaaccgtgt acgccaggag ggggggaacg tggtaatctc gccgggttac
     4261 acctttctcg ccggattggt gtacttcctc ggatttttgc cggcagcgtg gttttggtat
     4321 cgcatcctca actggctggg gcaagaggtg acattctttc gggcgctccg ggcctattgc
     4381 atcggccagt tgggcaagta tgtcccaggg aaggcttggg ttgtgattct gcggacatcc
     4441 ctggtacagg gcgagcaggt ccaacccgcc atggctgctg ccggagtttt cctagagaca
     4501 ctcacaatga tgtctgtggg tggagtcctc gcggtcctgt atttgatcat caatctgcgt
     4561 ggaaatcccc aattattttg gcttgccgtg ggactgtttg tcattgtgac gttacctacc
     4621 ctacctcctt tctttaggcc catcctgcgt tttatcgccc gcaagggaaa acgcgaggag
     4681 gttgttgtag ccatcgagaa gctgggtttt gcccgacttg cccaaggctg gttagcgatg
     4741 gtgttgctct ggacggcgta cggattgagt ctcagtctca cccttcaatc actggggatg
     4801 aagggagcaa tgatgttttc ccagttgccg aatatggtgg ctgccgtatc gctggcgacg
     4861 gtggccgggt ttttggtcct gtttcttccc ggtgggctgg gagccagaga gtttgttctg
     4921 attgccgtgt tagtccccgc cctgggcgac caggttgtct gttcgtctgg tatctcctct
     4981 gttgaattat tggccgtcat tggcgcagga ttattaaggg tcatttggtt gattacggag
     5041 atcgtggtag ccggcgtgtt ttggcttacc cttgtaatcg ggacattttt ccggcttccg
     5101 ggcgtgacag gtcgtcttca aaaagacgcc ccttcgccag aaccttgcgg tattgaggca
     5161 caaaaatgac gcgcggacta tcggttgtta ttcccgtgtt gaacgagtcg cctagtcttc
     5221 ggcagcttta cgaggaaatc aggcaagttg ccgaaaccca ccattacgac tacgagatca
     5281 tttttgttga tgacggctcc acagatgaca gttggcaagt tat
//
LOCUS       DAOPTR010000109         5319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_12570_length_5319_cov_3.584157, whole genome shotgun sequence.
ACCESSION   DAOPTR010000109 DAOPTR010000000
VERSION     DAOPTR010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12570_length_5319_cov_3.584157"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PKI05_03300"
     CDS             complement(<1..304)
                     /locus_tag="PKI05_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51254.1"
                     /translation="MRVSTRRLFSTRLVIFVVASVLAQLSLVNGELARAVEDQATAEQ
                     YAQLAEEIEGHFLKEVLPFWFPRCLDQEYGGFRPHFRSDGSLGPLNDKTIVFQSRMT"
     gene            complement(361..858)
                     /locus_tag="PKI05_03305"
     CDS             complement(361..858)
                     /locus_tag="PKI05_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HOA51255.1"
                     /translation="MGSENIPFDANEHGPSSEPPLVQGFIVFLESGISQLNNLLRRFD
                     VSRCRVLALHVTSQCGAAMVRIIVSHPEEGRIILERAGLPIADYRLVGVVLPDGVQPL
                     LRVYLPLIQANISVTATHVVPLTGRGKNAVLLEVDAPSRTIEILLAHGFQLIDEKMLA
                     QESPS"
     gene            1045..1539
                     /locus_tag="PKI05_03310"
     CDS             1045..1539
                     /locus_tag="PKI05_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008701405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOA51256.1"
                     /translation="MISADDNPGAHSDSPNIDGNPMNQPTGTIRKRGVVAVIAEEGKL
                     LLIRRAANVPAPGKWCFPGGAIEPGETPEMALIRELREELGVDIKPTRVLWESVTPWG
                     VFLMWWQASLPSNARLTPNPKEVAEVFWMKPHDLVAARDLLDSNRQFLEKVLSGEITL
                     DLET"
     gene            complement(1568..2923)
                     /locus_tag="PKI05_03315"
     CDS             complement(1568..2923)
                     /locus_tag="PKI05_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51257.1"
                     /translation="MLFRKLSLLLSAGLLLGLGVSLSARGEASFQQPAPRHVVVLLRN
                     RNVIEGELVAEGDIYRIRVSQGEIFVPHREVLAVADSIKALYEHERSRLFGREAEPHL
                     EMAIWCAEHGLEEEAWFELSQARLIDPRHPGLPLATRRVEMALLRKSQLSPSGKTRDE
                     STRAQASRAAQNAEAGARGENDKLGLPQVPDPNALFRGLPPGTGEQFVRVILPIVNHS
                     CASAGCHSKPDMNPQIRLLRVPTSRPPLRGEIAQNLQTVLGWVNFEHPGASPLLQVPL
                     EPHGGSPVPLFSGTAMKQYRELVDWVYAVTRRQAPPPPPGESSDAEAATLTGCSPPMS
                     AAGAAGVSLDGAFPILDFDVQQASHEISADSRGPKVVPATGSSPVNPVGAGGAVSAAT
                     PSDNPAGKPLPLLSDRPLFQSNPEPMPGIRWTFGPLESCPPVGSGVLTPELSPASSKD
                     H"
     gene            complement(3073..3726)
                     /locus_tag="PKI05_03320"
     CDS             complement(3073..3726)
                     /locus_tag="PKI05_03320"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOA51258.1"
                     /translation="MTAQSLNAEGVRLYQQARYQEALQRFGQALIYDPNDPSAYYNIA
                     AVYHRLWDLYHQPEDDSRAETYYQLALAKDPNHIAARRGLAVLLTARGRTQEAIAMLQ
                     NWAASWPQSAEPRVELARLYEELGDRTLAQKTLLEALAVEPRNVRALNALGHLREISG
                     EKELALAHYEQSLQVNQKQPELALRVSALRNELRVAQSTSPTQVSSNPWVPRTNRVR"
     gene            4098..4982
                     /locus_tag="PKI05_03325"
     CDS             4098..4982
                     /locus_tag="PKI05_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOA51259.1"
                     /translation="MTARLTRRSALGTLAGTVAAASLSTRVTAAGQDSPAPLKGNINH
                     SVSRWCFGKVPMEELCAFCKKIGIKSIELLGPKDFPIIKQFGLTCAMVSSGTSLTSGF
                     NKRENHDGLVAKYEEMIPIVAEAGFKNLICFSGNREPGLDDETGLKNCEVGIKRILGL
                     AEKHKVILVMELLNSKVNHKDYMCDHTAWGVELCKRIGSENFKLLYDIYHMQIMEGDL
                     IRTIRENIQYIGHFHTGGNPGRHEIDETQEIYYPAVMRAIVETGYTGFVGQEFVPSNP
                     EPAAALASLEKCIRICDV"
BASE COUNT         1232 a         1478 c         1482 g         1127 t
ORIGIN      
        1 acgtcattcg tgattggaag acgatggtct tatcgttgag cgggcccaaa gaaccatcgc
       61 tacgaaagtg tggacgaaag ccgccgtact cctgatccag acatcgcgga aaccaaaacg
      121 gcaaaacctc cttgagaaaa tgaccttcga tttcctcggc caattgtgcg tactgctcgg
      181 cagtcgcttg atcttctacc gccctggcca gctcgccgtt gaccagcgat agttgcgcca
      241 acacacttgc gactacaaag atgacaagcc gcgtggagaa caatctcctt gtgctaacac
      301 gcatcgttga ggctcccatc gtttgtcaga ccacacgtca ctcccgatcc cccaatcctt
      361 tcacgagggc gattcttgcg caagcatttt ctcgtcgatc aattgaaacc catgcgccaa
      421 gaggatctca attgtccgcg aaggggcatc gacttccagc aatacggcgt tcttgccacg
      481 cccagttaag ggcacaacgt gcgtggccgt gaccgatatg tttgcttgga tcaacggaag
      541 gtacacccga agaagtggct gaaccccgtc gggaaggacg acccccacaa gccgataatc
      601 ggcgatgggc agcccggccc gctctagaat gatgcgcccc tcttcgggat gactgacgat
      661 gatcctgacc atggcggccc cgcattgaga cgtcacgtgt agcgccagaa cccgacaacg
      721 gcttacatca aaccgccgta acaaattgtt taattgactg atcccggatt cgagaaaaac
      781 aatgaatccc tgaaccaggg gcggctcgct cgaagggcca tgttcgtttg cgtcgaaggg
      841 gatgttttcg gaacccatgg gatacgctcg tactagaaga cctgaacagc gggaacgcaa
      901 cccgctatta tgtatggatg atgattctgc ttagtttatc ttgtttccgc cccttgcgga
      961 acaatccaag tcactggcga attccccaaa tcgactagag ttttttcatg ccctgatttg
     1021 tttttccaac ttggaacgaa ttccatgatt tctgctgatg acaaccccgg tgcgcattcc
     1081 gattcgccca atattgacgg taaccccatg aatcagccca cggggacgat acgaaagcgc
     1141 ggggtggtgg ccgtcattgc tgaggaagga aaacttcttc tcatccgtcg ggcggcgaac
     1201 gtccccgctc ccggaaaatg gtgttttcct gggggcgcca tcgaaccggg cgaaacgccg
     1261 gaaatggcac ttatcagaga gctccgcgag gaattagggg ttgatatcaa accgacacgg
     1321 gtcctttggg aaagcgtgac accctggggt gtcttcctca tgtggtggca ggcgtctctc
     1381 ccttcgaacg caaggctaac accgaatcca aaggaggtgg cggaggtctt ttggatgaaa
     1441 cctcacgatc tggtcgccgc ccgcgatctc ctcgatagca accggcaatt tttggaaaag
     1501 gtcttgtctg gggaaataac gctagacctc gagacgtgat aacctcgcgg cacaaaggag
     1561 gtgatcctca gtgatcctta gagctagccg gagacaattc cggagttaaa acgccagaac
     1621 caaccggggg acagctctcc agaggcccaa acgtccagcg aatgccgggc atcggctcgg
     1681 gattggactg aaatagggga cgatcagaca agaggggcag cggttttccc gctgggttat
     1741 cacttggggt ggcggctgag accgcccccc ctgctccgac cggattgact ggcgaggaac
     1801 ctgttgccgg aaccaccttg gggcctcttg aatccgcaga tatctcgtgg ctcgcttgct
     1861 gaacatcgaa atcgaggatg gggaacgcgc catcgagaga aactcccgcg gcaccggcag
     1921 ccgacattgg gggactacaa ccggttaggg tggcggcctc cgcatctgaa ctttcgccag
     1981 gtggcggtgg cggcgcttgg cgacgggtca ccgcatacac ccaatcgaca agttcccgat
     2041 actgtttcat agccgtccca gaaaagagag gcaccggcga gccaccatgg ggttcaagcg
     2101 gtacctggag caatgggctt gcccccggat gttcaaaatt gacccagcca agcacggtct
     2161 gaaggttttg agcaatttcc cctcgcaagg gtggccgact ggttgggacc cgcaagagac
     2221 gaatctgagg attcatatcc ggcttgctgt ggcatccggc cgaggcacaa gaatggttaa
     2281 caatcggcag aatgacacgg acaaactgtt cccctgtccc cggtggcaga ccgcgaaaaa
     2341 gcgcgttggg atcagggacc tggggtaagc ccaacttatc gttttcgccg cgcgcccccg
     2401 cttcggcatt ctgtgccgcc cttgaggctt gcgccctcgt tgattcatcc cgtgtcttcc
     2461 cggagggact gagctgagac tttcggagaa gggccatttc cacccggcgc gttgccaagg
     2521 gcagccccgg gtgacgagga tcaatcaacc gtgcctgaga aagctcgaac caggcctcct
     2581 cttccagtcc gtgctctgcg caccaaatgg ccatttccaa atgcggttcg gcttcacgcc
     2641 caaagagtcg tgatcgctcg tgctcgtaaa gggccttgat cgagtcggca acggcgagga
     2701 cctcacggtg tgggacaaaa atttcgccct gagaaacacg gatgcgatag atatcgccct
     2761 ccgcaaccaa ttcgccctcg atgacgttgc gattccgcaa aagcacgaca acgtgtcgtg
     2821 gtgccggctg ctgaaaacta gcctcccccc gtgcggataa agatactccc agccctagca
     2881 gaagtcctgc actcaggagc aagctgagtt tccggaataa catatggtcc ccctcggatg
     2941 gtgcggtgcg tcatggacta acgagccggg gttttcattt ctttcccgct gcctagcggg
     3001 aaaggtagcc aaccaagcgg ctggcggtca atgtcgattc tttacccatc gaaaagagcg
     3061 tggccatcgc cactatcgaa cgcgatttgt ccgcggaacc cagggatttg aggaaacctg
     3121 ggtcggtgag gtgctttggg caactcgcag ctcattacgc aaggccgaga cccggagggc
     3181 caactcgggt tgcttttgat tcacttgtag tgattgctcg taatgggcca gtgccaattc
     3241 tttctccccg cttatttcgc ggaggtgccc cagggcattg agtgcccgaa cgttacgggg
     3301 ttccacagca agggcctcta gaagcgtctt ttgtgccaac gttcgatccc ctaactcctc
     3361 gtacagccgg gccaactcga cccgaggttc agccgattgc ggccaagatg cggcccaatt
     3421 ttggagcatg gcgatggcct cttgggttcg gccacgtgcc gtgagaagca ccgccagtcc
     3481 ccgtctggcc gcgatatgat tggggtcctt agcaagagcg agctggtaat aggtctccgc
     3541 ccgcgagtcg tcctccggct gatggtaaag gtcccacagc cgatggtaaa ccgctgcaat
     3601 attgtaatag gccgatggat cgttgggatc gtagatcagt gcctggccaa agcgctgcag
     3661 ggcctcctga tagcgggcct gttgatagag tcgcacgcct tctgcattga gtgactgcgc
     3721 agtcatactt gtgcagccgc caaggcataa aaggctggcg gcaaggacca tcataaggcc
     3781 ccgcgaataa cacgttcttt cgggttcaac gaaatgggga gctaccacac cggacgtcct
     3841 cggccatcac tgaaaccctg ctccaacaat catttccaaa ccacgggagg atacacaata
     3901 ggcccttttt ggggcaagac agttttttga ccggtaaatg cattcactta accgaaaaac
     3961 cgaaacccta cgaaaaatgg gttatttgag tcatctgccc cgttgtgtaa aatgagtctg
     4021 gttcatttga ccacctgaac agtcttagcg gctcttaggt gctacacaaa cactttctcg
     4081 aggataggag aactcccatg acagcacgtc tgacccgtcg ttcagccctt ggaactcttg
     4141 ccggaacagt ggcggctgcc tcgctgagca ctcgagtgac cgcggctggc caagactccc
     4201 cagcaccttt gaagggaaac atcaaccatt cggtctcccg gtggtgtttt ggcaaggtcc
     4261 ccatggaaga gctatgcgcc ttctgcaaaa agatcggcat caagtcgatt gagctcctgg
     4321 ggccaaagga tttcccaatt atcaagcagt tcggtctgac atgcgccatg gtgtcctcgg
     4381 ggacgagttt gacgagtggt ttcaataaac gggaaaacca tgatggtttg gtggcaaaat
     4441 acgaagagat gatcccgatc gtagccgagg ccggatttaa gaatttgatc tgcttctcgg
     4501 gtaaccgaga acctggcctg gacgacgaaa cgggcctaaa gaattgcgaa gttggaatca
     4561 agcggattct cggcctggcg gaaaagcaca aggttatcct cgtcatggag ctcctgaata
     4621 gcaaggtcaa tcacaaggat tacatgtgcg atcacacggc ttggggtgtc gaactgtgca
     4681 agcggatcgg ctccgagaac ttcaagctcc tttacgacat ttatcacatg caaattatgg
     4741 aaggcgatct gatccggacc attcgcgaga acatccaata cattggtcac ttccacacgg
     4801 gtggcaatcc cggccgacat gagatcgatg aaacgcagga gatttactac ccggccgtga
     4861 tgcgggcgat tgtggaaacg ggctacaccg gcttcgttgg ccaggagttt gtccccagta
     4921 acccggagcc ggctgccgcc ttggcgtccc tggaaaaatg cattcggatt tgtgacgtct
     4981 gattaagtat cccaaaaagt ctcactctcc ctccgggaga gggttgggat gagggcaagc
     5041 gaacgtcgga atcgattgag aaaaccggca gcgattcgag ctaccctcag ccccgatccc
     5101 tctcccgccg aacgcgtgag gggagattat gggataggct ctaactgcca atcgcggccg
     5161 agtccgcccg attcgcagcc tgaatgccac cacacgcccg gcctttgaca gcctggagac
     5221 ttcgtaatct aggcaatttg ttgcattgaa ggaaaagaag attgtgcgga ccacggcagt
     5281 acggtattta ctgctttgga tgaaggtgtt gttttccag
//
LOCUS       DAOPTR010000110         5315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_12584_length_5315_cov_3.657034, whole genome shotgun sequence.
ACCESSION   DAOPTR010000110 DAOPTR010000000
VERSION     DAOPTR010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12584_length_5315_cov_3.657034"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="PKI05_03330"
     CDS             complement(<1..822)
                     /locus_tag="PKI05_03330"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOA51260.1"
                     /translation="MIASSRREFLQTVLGGLALGRMAFASQETSAGGLPTRPLGNTGE
                     RVTIIGLGGWDVGAVREKKEALAIMHEAVDNGLTFFDNCWEYHQGRSEEWMGEAMAEG
                     KYRDKVFLMTKVCGRDAKTAQQNLEDSLRRLRTDHIDLWQFHGIQWDDDPDLIFGENG
                     ALAYALKAQKEGKVRYIGFTGHKHPRFHLAMLQKPYEWASVQMPINVLDAHYESFQKQ
                     VLPECVKRNIGVIGMKALGSQNGRIPRELGIPAPLLRRFALSLPITSLICGIQSRE"
     gene            complement(1048..1923)
                     /locus_tag="PKI05_03335"
     CDS             complement(1048..1923)
                     /locus_tag="PKI05_03335"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HOA51261.1"
                     /translation="MEKRLTRRVALSSLGLISGGILASGCTKGLVAPVSAEPVTPAAN
                     GNGLGASSEADHGWKYAPLNAEEIADRAYAMYPDGGCMYSMVGSVISALAERFGEPFS
                     QFPYSMMRYGEGGVGKWGSLCGVVNGAAALIGLFYPEDQNTREKLIEAFAYWYESSEL
                     PVYVPKDAPQSMEIPKSVAGSILCHFSVGAWCKVSGYPAFCKEKTERCRRLTADGVRF
                     LVTILNESHMQTNRELPTSPTAKACNDCHGKAQLADAMVKMNCQSCHDLDPSHSVQPV
                     TNGTEFNPAQIRSGR"
     gene            2562..3101
                     /locus_tag="PKI05_03340"
     CDS             2562..3101
                     /locus_tag="PKI05_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51262.1"
                     /translation="MLGFVHPIIALEQSKFQGGLLVLNQSGDPVEFRCTVPVRTTRLQ
                     QILWGQRLVAHVLGQLILRPLIQSAKTQIAVVLVEREEFLPVRGDLSVPLLLIRRQDH
                     PSDRIQSPNTVTIAHVSPGKEALLLQSAWGFEEDLEVGCRLVSEFLKTCHPLEAFQRI
                     EQALHILKPKLEEQVRVTG"
     gene            4034..>5315
                     /locus_tag="PKI05_03345"
     CDS             4034..>5315
                     /locus_tag="PKI05_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOA51263.1"
                     /translation="MWKAVTTTLNRALTSPLLWGSAAFVAFQFFLKADLPGGDFLRRY
                     TAGHPIEYIETFLFFLALAALIHKGLTILYEWSTLDQPWLSGPLNAPADRLLGDLDAL
                     PEVLHTSPVFRQLRAGLSYLCRGGTAQELDQHLAFAAEQEADERRHHYAFVRLVIWAI
                     PILGFLGTVVGITLSLGNLAPQQLEETLPAVMAGLMVAFDTTALALALSMILMLVQYV
                     VERSEQRLLAAAGEKVENLLLSRFPAQSVPGRELTAVRVMLEEVLTTCRHLVETQVTL
                     WQQSLEQAQKQWQGTWSNWQEQLGNRLATALENALQRHADRLHEAEAQLFERLQQREL
                     EAAQDLTARWKSLLTEASRQQEEWASLQKILQGHVTAWQTLASETQHLARLEELLQQN
                     LTAIGSAKHFEEALASLAAAANLLSAHLHRIPLEP"
BASE COUNT         1107 a         1642 c         1465 g         1101 t
ORIGIN      
        1 ttcccgcgat tggatgccgc aaatcagact tgtgattggc agcgacaggg caaaacggcg
       61 caataggggg gccgggatgc ccagctctcg cggaatccgc ccgttctgag accccagcgc
      121 tttcatccca atcacaccga tattgcgttt cacgcactct ggcaggacct gtttctggaa
      181 gctctcataa tgggcatcca ggacgttgat cggcatttgc acgcttgccc actcataagg
      241 tttctggagc atcgccagat ggaaccgtgg gtgcttgtgg ccggtgaacc cgatatagcg
      301 gacttttcct tccttttggg ctttgagggc atacgcgagg gcgccatttt ctccaaaaat
      361 caggtccgga tcatcatccc actggatgcc atggaattgc cacagatcga tgtgatccgt
      421 cctcaggcga cgcagactgt cctccaggtt ttgctgggcc gtcttggcat cccgaccgca
      481 gactttcgtc atcagaaaaa ccttatcccg atacttccct tccgccatgg cctcgcccat
      541 ccattcttcg ctgcggcctt gatggtattc ccagcagtta tcgaaaaatg ttagcccgtt
      601 atcgaccgcc tcgtgcatga tggccagggc ctcctttttc tcccgcacgg ccccaacatc
      661 ccagcccccc aggccgataa tcgtgacccg ttcacccgta ttgcccaatg gacgggtagg
      721 aagccctccg gcactggtct cttgcgacgc aaatgccatg cgaccgaggg ccagacctcc
      781 cagaactgtc tgcagaaact cgcggcgaga tgatgcgatc atgggcaaga ttcctttaca
      841 aagccaaccg gtaacgttct cttccccaag ggcttgccaa ttttacgcac gggtgcccaa
      901 agcgaaaagt cgtttaccct agaccgctcg tctcgaaaag ccgggaactg ctcaccccca
      961 ggatgagtga actgctaagc gggtctgctc tccacaaggc ctggaccttc cgcggcccgg
     1021 ggctacaaca tgacaaccga tgtggcgtca acgaccggaa cggatctggg cagggttaaa
     1081 ctctgttcca ttcgtcaccg gctggacaga gtgcgaagga tccaggtcat gacaactctg
     1141 gcagttcatc ttgaccatgg catccgccaa ttgcgctttc ccatgacagt cattgcacgc
     1201 tttggccgtt ggggaagtgg gaagctctcg gttagtctgc atgtgcgact cgttcaaaat
     1261 ggtcaccaag aaccgcacac catctgcggt cagacgacgg cagcgctcgg ttttttcttt
     1321 gcaaaaggcg ggatagcccg agaccttgca ccaggcccca actgagaaat gacaaagaat
     1381 cgacccggcg acactctttg ggatctccat gctttgcgga gcatccttgg gcacatagac
     1441 gggtaattcc gaggattcgt accaataagc gaaggcctcg atgagtttct cgcgagtgtt
     1501 ttgatcttct ggataaaaca agccgatgag agcggcagcc ccgttgacaa caccgcacaa
     1561 agacccccat tttccgacac caccctcccc gtaacgcatc atgctgtagg gaaattggct
     1621 gaacggctct ccgaaccgct ccgccagcgc cgagatgacc gagcccacca ttgagtacat
     1681 gcaacccccg tccgggtaca tcgcatacgc ccggtcggct atctcctcgg cattgagagg
     1741 cgcgtacttc caaccgtggt cggcttcgga ggaagctccc aatccattgc cgttggctgc
     1801 cggggtgacc ggctccgccg aaacaggggc caccaaacct tttgtgcacc cagaagccaa
     1861 aatcccgcct gaaatcaacc ccagtgaaga aagcgctacc cgtcgcgtta atctcttctc
     1921 catagcttcc ccggtaaaac agcgagtacc ctgacttatc gcgaacaacc tttagaaccc
     1981 accttgcctt ggcgtggatg cacagtttac caaatatgca tgaacgacgc aaggaatgtc
     2041 acagaagggt gccgccgata ttttctgccg ggtctccgcg acacggcgcg agacaagcag
     2101 catttccgcg gccctatcga gagcccatct ccaagttccc tctccctctg ggagagggtt
     2161 agggtgaggg cacccgaccg tcggagtcca ccgagaaaac cggcagcgat tcgagccacc
     2221 gtcactcctg gcccctctcc cgcggaacag gagaagggag gttgtaggat actaggcagc
     2281 aaagacaccg agtcaggaca tccaatcccg ccgggcattc cttgcacgtc cccgatggcc
     2341 tcccaagcct ctgggaggga cgcggtcccc cgtgttcgtc gcgtctgggc accgcgggcg
     2401 caagtccgac cggcgtcaaa atcgtccggc ggcaacattc tctgccgaaa acgtttgacg
     2461 cgagcgggcg gggcaaagtt taatgaaaag atgcggcgac gccagtccac acgtcatcgc
     2521 cgacgcttgg tgaggccaag ctttgggagg agcggcagta aatgcttgga ttcgtacatc
     2581 ccatcattgc cttagagcag tccaagtttc aaggcggtct gctggtcctc aatcagtccg
     2641 gtgacccggt ggaattccgc tgtacggtgc ctgtgaggac aacccggtta caacaaattc
     2701 tttgggggca gcggctggtt gctcacgtct tgggacagct gatccttcgg cctttgatcc
     2761 agtccgcgaa gactcaaatt gccgtcgttc ttgtggagag ggaggagttc cttccagtgc
     2821 gaggcgattt gtccgttccg ctcttattga tccgacgtca agaccatccc tccgatcgga
     2881 tccagtcacc gaacacggta accatcgccc acgtgtcccc cggcaaggaa gccttattgc
     2941 tgcaatctgc gtggggtttt gaagaggacc tcgaagtcgg ttgccggctg gtgagcgaat
     3001 tcctcaaaac ctgtcacccc ctggaggcct ttcagcgaat agaacaagca ctccacatcc
     3061 tgaagccgaa acttgaggag caggtgagag tcacggggta atcgctcggg cgccacgcgg
     3121 ttggttgagc cggctcatct ttaaagccta tcccatcatc tcccctggcg cggtcggcgg
     3181 gagaggggtc gcgggtgagg gtggctcgaa tcgatgacgc ttttctcaat cgattttgac
     3241 gttcgcttgc cctcacccca gccctctccc agagggagag ggggactttt ttaggataca
     3301 cccttgatat ccgcgaccaa cccggcgttt gcaacacctg gacggcgggg acaccgtccc
     3361 tcccaatttc gccccgggag gatcatcggc cccgatgacc cgggcaccga tcaaaacgca
     3421 cgcccatttt ggcgatcaga ctcgggcgct tgtttgcgtt ctggggcagg cacgggggcc
     3481 tgcccctacg ggggtcgatg gactcataaa cgggtagggg caaccccctg tggttgcccc
     3541 gtttcgccac agcgccaccc gggagggtcg ccgtcctcgg cgaccaaatc atcggacaac
     3601 gcgaatcccc ggtagggaca agccgtcaac accgggacgc acaatcgcac gggcaagatg
     3661 cccgtgccac gcgatttcaa cgggcttcta ttccttcggg gcgtcaaacg tgcgggcaac
     3721 cccctgtggt tgcctttttc tttttgagaa accttaacga ttttgccgat tattcaaaag
     3781 gcgctgaaac ggctgccagg catgaccttt tcggctggca atggggaccg ggttccagct
     3841 ggcgcccatg tcggtgtcgc ggaatcactg tggtgcgaga tcgagacagc acgacagggc
     3901 aattgcgatt gacagaagac cgtttccgct aggacaatcg ctctccctac gccccgacct
     3961 aggggacaac cgtgggggat actaaaccga cgatgtttct tcacgagatc gagacccaag
     4021 tgagtgccct tccatgtgga aagcagtgac gactaccctt aatcgtgcct tgacctcccc
     4081 attgctgtgg ggctcggccg cgtttgtcgc atttcagttt tttctcaaag ccgacctgcc
     4141 gggtggtgat tttctccgcc gctacaccgc gggtcacccg attgagtaca tcgaaacatt
     4201 tctttttttt ctcgcactag cggccttgat tcacaaggga ctgacaatcc tctacgagtg
     4261 gtccaccctc gatcaaccgt ggttatccgg tccgcttaac gccccggcgg atcgtctcct
     4321 cggcgatctc gatgcgcttc ctgaggtcct ccacaccagt cccgtgtttc gccaactaag
     4381 ggccggcctg agttacctct gccggggtgg caccgcccaa gaattggatc aacacctggc
     4441 ctttgctgcc gagcaggagg ccgatgaacg ccgtcatcac tacgccttcg tgcggttggt
     4501 catctgggcg attcccattt tgggttttct gggcacagtg gtggggatca cgctctcgct
     4561 cggtaatctc gcaccacaac agttagagga gaccttgccc gcggtcatgg ccggattgat
     4621 ggtggctttc gacacgaccg ccctggccct ggccctgtcg atgatcctca tgctggtaca
     4681 atacgtcgtg gagcgctctg agcagcggct cctcgccgct gccggggaaa aggtcgagaa
     4741 cctgctcctt tcccgcttcc ccgcacagtc ggtgccggga cgggagttga ccgccgtgcg
     4801 ggtcatgttg gaagaagttc tgacgacctg ccgccacctg gtggaaaccc aggtcaccct
     4861 ctggcagcaa agcctggaac aggcccaaaa gcagtggcaa ggcacctggt ccaattggca
     4921 ggaacaactg gggaacagac ttgccacggc cctggaaaac gccctgcagc gccatgccga
     4981 ccggcttcac gaggccgagg cccagctttt cgagcgactt cagcagcggg aattggaagc
     5041 cgcccaagac ctgaccgcac ggtggaaatc actgctaaca gaggcctcgc gtcagcagga
     5101 agaatgggcc agcctccaga aaatccttca gggacacgtg acggcttggc agacacttgc
     5161 ctccgagacc caacacctcg ctcggttgga ggagttgctc cagcagaacc taacggccat
     5221 cggttctgcc aaacactttg aggaggcttt ggcaagcctg gctgcggcag ccaacctgtt
     5281 aagtgcccac ctccaccgta tccccttgga accac
//
LOCUS       DAOPTR010000111         5312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_12594_length_5312_cov_3.160548, whole genome shotgun sequence.
ACCESSION   DAOPTR010000111 DAOPTR010000000
VERSION     DAOPTR010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12594_length_5312_cov_3.160548"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PKI05_03350"
     CDS             <1..717
                     /locus_tag="PKI05_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA51264.1"
                     /translation="DTRPEAQQDFARMVRAYLATIKSVDDSVGEIVATLKATGQFDRT
                     IVIFTSDNGFLLGEYGMMDKRTMHEPSIRIPLVVRYPPVIKPGTIIKEMVLNVDLAPS
                     ILDLCGAPPLPKTHGRSWRPLLEGRTEGWRKSFYYEYNYEKQFPYTPNVRGVRTERWK
                     YVHYPTGDGTPDRHKAELYDLQNDPEERYNLIDDPRYQSVLEELRQELLRLQKETDAL
                     PDRMPIDEGIKTELPEQSIR"
     gene            811..2100
                     /locus_tag="PKI05_03355"
     CDS             811..2100
                     /locus_tag="PKI05_03355"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA51265.1"
                     /translation="MSTHKLSRRYVLKSLATGAVSAVAIPTVIPRSALGDDATPGANE
                     RITVGVIGTGGRSRLLIEQLPSQGQILAVCDCFLERAEQVNAEKGQGRWAVYQDHRQL
                     LERKDIDAVIVGTPDHTRVLVCIHACQAGKDIYAEKPLTLYIREGRVLVNAVRKYKRV
                     FQVGSQQRSMAMNRIACEFIRSGKLGKIRVVQGCNYTGPRFYEGLPEEPIPAKLDWDR
                     WLGQTPYRPYNRQLHYGWMAWWDYSGGEMTNWGAHGLDQIQWALGKDDTGPVDIRPQG
                     SPRDMNAAVHMKYADGTPVRLELSPGPAGGAIFSGENGRIEINRNKFTCNPPDLIKDL
                     PPQEEIDKWRDEVALWQAKYHMENWMNCIRTREKPVADVEIGHRSITIAHLANIARYL
                     GRRLQWDPVKEVFIGDDEANSLVERPQRAGYELPQIV"
     gene            2611..4794
                     /gene="nuoF"
                     /locus_tag="PKI05_03360"
     CDS             2611..4794
                     /gene="nuoF"
                     /locus_tag="PKI05_03360"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOA51266.1"
                     /translation="MKQDVWDALKNLREKAERVYQERMGQGQIFIQVGSATCEHAAGA
                     IEVADEFRKHIAASGRNDIILRRTGCTGRCSREPIVGVFRAGEMPIKYQLVNRELVHK
                     IFTQHVLGGEPLLDHVLDGPIERLSKYEILNCGSLRCGWKGEQICGGPMANQLKAMGV
                     PPDVCRVTSASCFGACSIELAGKCTHILVRPQNVLYRITSLADLEEVVREHIGKGQIV
                     ERLRVKEEPVSQDFFELYGEIAFFNRQTRIALRHNGIIDPTSIEEYFHYRGFEALAKV
                     LAQDDPDWIVDEVTRSKLRGRGGGGFPTGQKWALARKSPEKTRYIICNADEGDPGAFM
                     DRSMLESDPFNVIEGMIIGAFAIGAHRGFVYVRAEYPLAVERVDHAIRMCREWGVLGK
                     NILNSGFDFDIEIRLGAGAFVCGEETALIHSIEGERGQPRIRPPYPTEVGLWGKPTVI
                     NNVETFANIPAIIIYGADWFSRIGTNASGGTKVFALAGKVKQTGLVEVPIGITLREVV
                     MDIGGGCPNDKKVKAVQTGGPAGGCIPASMLDLRVDFDTLNKAGSIMGSGGMIVLDED
                     DCMVDIAKFFMRFSEDESCGKCTPCREGTTRMVEILERITTGKADESDLEKLERLAKL
                     VRKASLCGLGRAAPNPVLSTLTHFREEYLAHVRDKRCPAKKCTALIHYEIDPEKCVGC
                     TACARNCPVECISGERRQPHVIDQSRCIKCGRCFQVCRFDAVMRS"
     gene            4947..>5312
                     /locus_tag="PKI05_03365"
     CDS             4947..>5312
                     /locus_tag="PKI05_03365"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOA51267.1"
                     /translation="MIAKRRKKITLTIDGNQVTVPEGTTIMQAAEQIGIRIPRLCYHP
                     DLSLAGSCRVCIVEVKDMGHYMASCSSPVWEGMEVRTNSPEIREARRDIVELLLDNHP
                     MDCQTCERDGNCELQNLAYS"
BASE COUNT         1244 a         1338 c         1572 g         1158 t
ORIGIN      
        1 gatacgcgtc ccgaggccca gcaggatttt gccaggatgg tccgggccta tctcgccaca
       61 atcaagtcgg tggatgacag cgtgggcgaa atcgtagcaa ctctaaaggc aaccggtcag
      121 tttgatcgga cgatcgtgat tttcacatca gataacggat ttctgctcgg agaatacgga
      181 atgatggaca aacgcacgat gcacgagcca agcatccgga ttccccttgt tgtgcggtat
      241 ccgccggtga ttaaacctgg gacgatcatt aaggagatgg tgctaaatgt ggacctggcc
      301 ccaagcatcc ttgatttgtg cggtgcgccg cctttgccaa agactcatgg ccggtcttgg
      361 cgtcccctcc tggaaggccg cactgaagga tggcgcaaat ccttctatta tgagtacaac
      421 tacgagaaac aattccctta cacacccaat gtgcggggcg tccgcacgga acggtggaaa
      481 tatgtccatt atccgaccgg cgatgggacg ccggatcggc acaaagccga attatacgac
      541 ctgcaaaacg acccggaaga gcgatacaac ttgattgacg atccgcggta ccaatcggtt
      601 cttgaggagc ttcgtcaaga gctgctgcgt ttacagaagg aaacagatgc ccttccggac
      661 cgcatgccta ttgatgaagg cattaagaca gaactacctg agcagtccat ccgttgagct
      721 attgtgctcg tatgggtcaa accgtttgat gatcatccta gtggaagatt cccgacacaa
      781 tttactcctt gagataggaa ctgtgaaacc atgagcaccc acaaactttc gcgccgttat
      841 gttctgaagt cattggcaac tggtgctgtt tcggcggtgg ccattccgac cgtgatacct
      901 cgttccgccc tcggagacga tgcaaccccc ggtgccaatg agcggatcac ggttggtgtg
      961 atcggaacag gtgggcgatc tcggctgctc atcgagcaac tcccctctca agggcagatc
     1021 ctcgccgtat gcgattgctt cctggaacgg gccgagcagg tcaacgccga aaagggccaa
     1081 ggccgttggg ccgtttatca agatcatcgt caactgctgg agcggaaaga tatcgacgcc
     1141 gtcatcgtgg gcacgcctga ccatacccgc gtgctggtgt gtatccatgc ctgtcaggca
     1201 ggtaaagaca tttacgccga gaaacccctg acgctgtaca ttcgcgaagg tcgtgtcctt
     1261 gtcaacgccg tgcgtaagta caagcgggtc ttccaggtgg gaagtcaaca gcgatccatg
     1321 gccatgaacc gaattgcctg tgagtttatc cgctcaggca aactgggcaa gatacgcgtg
     1381 gttcaaggct gcaattatac agggccgcgc ttttacgagg gcctgcctga agagccgatt
     1441 cccgcaaagc tggattggga ccgatggcta ggccagacgc cttatcgtcc gtataaccgg
     1501 caactccatt acggttggat ggcctggtgg gattactcgg gtggcgaaat gacgaactgg
     1561 ggtgcccacg gcctggacca gattcaatgg gcattgggca aagatgacac cgggcccgtt
     1621 gatatccgtc cgcaaggaag tcccagggac atgaacgcgg ccgtccatat gaaatacgct
     1681 gatgggacac ctgtcaggtt ggagctatca ccggggcccg cgggcggtgc tattttcagc
     1741 ggggaaaacg gacggattga aatcaaccgt aacaagttca catgcaatcc gccggacttg
     1801 atcaaagatt tgccacctca ggaagagatc gacaaatggc gcgatgaggt tgccctttgg
     1861 caagctaagt atcacatgga aaactggatg aactgcataa gaactcggga aaaaccggtg
     1921 gcggacgtgg agatcggcca tcgctcgatc accatcgcgc acttggcaaa tatcgcccgg
     1981 tacctaggtc gccgactcca gtgggacccc gtgaaagagg tctttattgg cgacgacgag
     2041 gccaatagtt tggtggaacg accacaacgg gcgggctacg aacttccaca aattgtctaa
     2101 agatagggag actgtgccca agaggcccac gttaactggg ccgggcatat ttgtgaattt
     2161 ttaaagtgcc gtgaccgtaa gtgtaagctg gcggccgctt gggtgttcgg ctcaagcagg
     2221 tcaggttaca ctcgcggtgg ggacgggcta gttcatgctg cgggtcagtt gaccaagctg
     2281 gcatcggcaa cggagggcgt gccgcgccga acaacgttga gataggggac gtactacaaa
     2341 aggggacggc gagagcatcg cccatgctta tctatggcgt tcgtccgaaa gacgctacaa
     2401 taaatgcctg ttgtatttga tcccacgcac tgatgacatc gttaggaaat cattgcgaga
     2461 gacgatcatt ggattcgttc ttgaaaaagc tgctctctag aagctggatg ctctgtcttt
     2521 tttgggaaaa gatggcgttt tctgttcaag attgtcaaga ttagccgaat cctttgtcgc
     2581 accgaggcca ggattagtcg aggctgagcc gtgaaacaag acgtttggga tgcactaaag
     2641 aaccttcggg aaaaggccga gcgggtttat caggaaagaa tgggtcaggg gcagatcttc
     2701 attcaagtgg gatcggccac ctgtgaacac gccgctggcg cgattgaagt tgcagacgag
     2761 tttcgaaaac acatcgcggc ctcgggtcgc aacgacatta tcctccgtcg aacaggttgc
     2821 accggccgat gtagtcgcga gccgatcgtg ggtgtcttcc gcgccggcga aatgcccatc
     2881 aagtatcagt tggtcaatcg ggagctcgtt cacaagattt tcacccaaca cgtcctcggg
     2941 ggtgaaccgt tactcgatca cgttctggat ggcccgattg agcggctttc aaaatacgag
     3001 attttgaact gtggttccct acggtgtggc tggaaagggg aacaaatttg cggcggcccc
     3061 atggccaatc agttgaaagc catgggggtc ccgccggatg tttgccgggt aaccagtgca
     3121 agctgctttg gtgcatgcag cattgaacta gcgggaaaat gtacccatat tctcgttcga
     3181 ccgcaaaacg tcctgtaccg catcacgagc ctggccgacc tcgaggaggt cgtgcgcgag
     3241 catattggaa agggacaaat tgtcgaacgc ctccgcgtca aggaggaacc agttagccaa
     3301 gacttcttcg agctgtacgg cgaaattgcc tttttcaacc ggcagacccg gatcgccctt
     3361 cgccataacg ggatcatcga cccgacaagt atcgaggaat acttccacta tcgtggtttt
     3421 gaggcgttgg cgaaggtcct tgctcaagac gatcccgact ggattgtgga tgaggtgacc
     3481 cgttcgaaat tgcgtgggcg tggtgggggt ggctttccca cgggtcaaaa gtgggcgttg
     3541 gcacgcaaat ctcccgagaa gacccgctat atcatttgca acgccgacga aggcgatccc
     3601 ggggccttca tggatcggag tatgctcgaa tccgatccgt tcaacgtcat tgagggaatg
     3661 attatcgggg cctttgccat tggggcccac cgaggatttg tctatgtccg ggcggagtac
     3721 cccctggccg tggaacgggt ggatcatgcc atccgcatgt gccgcgaatg gggagtcttg
     3781 ggcaaaaaca ttttgaactc gggtttcgac tttgacattg aaatccggct cggtgccggg
     3841 gcctttgtct gcggagaaga gaccgcgctc atccactcca tcgaaggtga gcgaggacaa
     3901 ccacgcattc gacctcccta tcccaccgag gttggtttgt gggggaagcc caccgttatc
     3961 aataacgtgg agacattcgc caacatcccg gcgatcatca tctatggggc tgattggttt
     4021 agccgaatcg gtactaacgc cagcggtggg accaaggtgt tcgccttggc tggaaaggtc
     4081 aagcaaacag ggctggtcga agtgcccatt gggattactc tccgtgaagt cgtcatggac
     4141 attgggggtg gctgccccaa tgacaagaag gtcaaagctg tccaaacggg tgggccagca
     4201 ggcgggtgta tcccggcctc catgctggat ctgcgggtcg atttcgatac gctcaacaaa
     4261 gccggctcga tcatgggctc cgggggcatg atcgtcctgg atgaagacga ctgcatggtc
     4321 gatatcgcca agttttttat gcgattttct gaagacgaat cgtgcggcaa gtgtacgcct
     4381 tgccgggaag gcacgacgcg gatggtcgag attttggaga ggatcaccac ggggaaagcc
     4441 gacgaaagcg atctggagaa gttggaacgg ttggccaagt tggtgcgaaa ggcgtcgctg
     4501 tgtgggctgg gaagggccgc tcccaaccct gtgctgagca cattgaccca ctttcgggaa
     4561 gaatacctgg cccatgtccg ggacaagagg tgccccgcca agaagtgtac ggccttgatt
     4621 cactatgaga ttgatccgga gaagtgcgtg ggctgcacgg cttgtgccag gaattgcccc
     4681 gtggaatgca tctcgggtga gcggcgacaa ccgcatgtca tcgatcaaag tcgttgtatc
     4741 aagtgtggac ggtgtttcca ggtttgtcgc ttcgacgcgg ttatgcgatc ttgagcagca
     4801 aatcgtaaaa cgaggtactt cctcggggtg tttgcaaggc ttggtgatat gctgagcaga
     4861 ttgagcttgt agaaatgtct cgctgattcg taagcgtcaa gatggtgtta acaggtcgaa
     4921 aacactaggc tgcggacagg cgatccatga ttgctaaacg gcgaaaaaag atcactttga
     4981 caattgatgg taaccaggta accgttccgg aaggcaccac catcatgcag gccgcggaac
     5041 aaattggaat ccgtattccg cgattgtgtt accacccgga tttgagcctc gccggatcgt
     5101 gtcgcgtctg cattgtggaa gtcaaagata tgggccatta tatggcctcg tgcagctctc
     5161 cggtttggga agggatggag gtccgcacca attccccgga gattcgggag gctcgccgcg
     5221 atattgtcga gctacttctc gacaatcatc ccatggactg ccaaacatgc gaacgggacg
     5281 gcaattgtga attgcagaac ctggcctatt cc
//
LOCUS       DAOPTR010000112         5305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_12622_length_5305_cov_3.641905, whole genome shotgun sequence.
ACCESSION   DAOPTR010000112 DAOPTR010000000
VERSION     DAOPTR010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12622_length_5305_cov_3.641905"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PKI05_03370"
     CDS             <1..334
                     /locus_tag="PKI05_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOA51268.1"
                     /translation="FRQQPELLDKLATGQVFPAEQAKAEGLVDEIGFLEDAVDRAIEL
                     ANLDPDNVRVVKYEKELTLMDVLLGETVQVHIGPAGMDWKSLLEWSVPRAYYLFYQGP
                     VLIQSGLR"
     gene            complement(326..1252)
                     /locus_tag="PKI05_03375"
     CDS             complement(326..1252)
                     /locus_tag="PKI05_03375"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOA51269.1"
                     /translation="MARILVVDDSPLDRQWLAGLLATIPDAEIDEAADGEEALRIVLE
                     RQPDIVVTDMQMPKLDGLQLVTAIRRGSPHIPVILVTGHGSEESALQALRVGAASYVN
                     KEWIPQRLIPTVKQVLEAAQHRAMISRVSRFLTGCHFGFVLENDCQLLRPVLEFVHDS
                     LECFDLDLTEITRVNVALQEALSNAIFHGNLALSSRLREEDESRFFQLARERRQRPPY
                     RDRRVFLEGELTREKISFAIRDEGPGFDPSLLPNPHRTEALDCACGRGLLLIRSFMDE
                     VSFNARGNEIRLVKYLRREDPSQEHLSPTGLS"
     gene            complement(1398..5174)
                     /locus_tag="PKI05_03380"
     CDS             complement(1398..5174)
                     /locus_tag="PKI05_03380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOA51270.1"
                     /translation="MSHNGVNDRSVESPPTAVSWWARARVWIATLVVLAIALAVSGYI
                     WRVTAQRETMRIVSQCSHQSQLQVQNIEFKLQQSQNLLQFLRSFFAASEDVHRYEFAE
                     FCRSHLDNIPVVKFAAWAPVTYPDGFETLAECADRHGVANFQPWELDEAGQRTFVQTR
                     PQHFPILFIEPSPPFDRLVGLDIASIPSWAEALEETIMIGEVDVHFLEGVPAIDEVVF
                     EADSGVGSSSLLESEEETFSTQYSRSSAAGLAPRKYLAICSPVFFGGYIPENPFERLT
                     QCGGVLVCVVDVQELLRPLKRWAAVEGINFRLVDLQGTSQHVVEAWERPSSRTERLLQ
                     TYPTSSWDIRLPGRVWRVITQPTATYVASRRGSGSWGVLAAGTLCSFLLALYVNVIGG
                     RSLRIQQLVAEKTHELRETNRRLEAEIEQREKVEKGLRDSEALYSSLVESLPLHVLRK
                     DREGHFTFANTAFCHLVGRSREEIYGKTDFDFYPPDLASKYRRDDRRVMETGEIFEDI
                     EEYEKDGEIRYVQVLKSPVRDHAGNIVGVQVAFWDVTEKVAVQRALEKAKQAAEEANR
                     AKSDFLANMSHEIRTPMNAILGMAQLLEQTPLNAEQKEYLRIIREAGDNLLALINSVL
                     DFSKIESGRIELDSVDFNARELIGDTMKALAVRAHKKQLELACRVAPNVPEELVGDPV
                     RLRQILINLVDNAIKFTEQGEVVVDVTCDECTTSDVVLRCSVRDTGIGIPPEKQQVIF
                     AAFEQADKSTTRKYGGTGLGLAICKRLVNLMGGTITVESQPGVGSTFTFTARFRRPSE
                     TLPKGLPVDLREYADRPILVVDDNATNRVILSEMIANWGLKSELAASGEEALRLVTDE
                     AGQVRFLVVITDMRMPGMDGMALIRQLRRRFGAEQLAIIVLSSEDRPTDSEFFRRFEV
                     SGVLRKPVKQSELYNILAEILGLAFHPESKTTIPASFPELPPLQILLVEDSPFNQQVA
                     IGVLEKRGHTVTVASNGKEALEILRNRSFDLILMDIQMPEMDGLTATKEIRAREERLQ
                     QPRTPIIAMTAHALKSDREAALKAGMDGYVAKPIVVEEFFQTIADVLHQVRGINLGDT
                     QALPTLEMSTSSPDGSISPTPTAADTPAPQENSLPSPPTSEVNSPESALETRPGESTH
                     VDWQHALRAAGGDRELLRALVNTFIQEMPRQWENLEKAVSQEDWPTARRATHTLGGSF
                     RHFGARRAADVAYQAEQALAQGGSQRDLTEPLQQLYHCLQEILRELREARDRGI"
BASE COUNT         1142 a         1513 c         1473 g         1177 t
ORIGIN      
        1 attccggcag cagccggaac tgcttgataa attggcaacc ggtcaggtgt ttcccgccga
       61 gcaggccaag gcggaggggc tcgtggacga gatcggcttc ttagaggatg ccgtcgatcg
      121 ggcgatcgaa ttagccaatt tggatcctga caatgttcgc gtcgttaagt acgagaaaga
      181 attgacgtta atggatgtgc tcttgggcga gacggtccag gttcatattg ggccggccgg
      241 catggattgg aagtctctcc tggagtggag tgtcccccgg gcctattacc tcttttatca
      301 aggcccggtc ttaatccaga gtggtttacg ataagccggt cggggagaga tgctcttgcg
      361 agggatcttc ccgccgcaaa tatttaacca agcgaatttc gtttccccgg gcgttgaagc
      421 tcacctcgtc cataaatgat cgtatgagaa gcagcccccg gccacaggcg cagtcgagcg
      481 cctctgtgcg atgcgggttg gggagcaaag aaggatcgaa gcccggtcct tcgtcgcgga
      541 ttgcaaaaga aatcttctcg cgcgtcagtt ccccttccag aaagacgcga cgatcccggt
      601 aaggtggccg ctgacgacgt tcccttgcca actgaaagaa acgggactca tcctcctccc
      661 gtagccgcga ggaaagtgcc aagttgccgt ggaagatagc gttggacagg gcctcttgaa
      721 gcgctacgtt gacgcgcgtg atctcggtca ggtcaaggtc aaaacactcc agtgaatcgt
      781 gaacgaattc taagacgggc ctaagcagtt ggcagtcatt ttccaggaca aagccaaagt
      841 ggcaacccgt caggaatctc gacacccgcg agatcatggc gcggtgctgg gcggcctcca
      901 gaacctgctt gacagtggga ataagtcgct ggggtatcca ctctttgttg acgtaacttg
      961 cggcgccaac gcgcaatgct tggagtgccg attcctcgct gccatggccc gtgacaagga
     1021 tcacgggaat atggggggac cctcgccgaa tggccgtgac cagttgcagc ccatcgagct
     1081 tgggcatttg catatctgtc acaacgatgt caggttgcct ctccaggacg atgcggaggg
     1141 cctcctcgcc gtcggctgct tcatcaatct cagcgtcggg aatcgtggcc agtagccccg
     1201 ccaaccactg acggtcaaga ggggaatcat cgaccactag aatccttgcc atgtgcggat
     1261 ccttcgcaag cgttcacgat ctcagggcac ggtcatgggc gacacattct tggacaacgc
     1321 tcttcttttt cctggaagac ccaactttct tgccttatat cttccccttt ggggagaaat
     1381 ctttggacat cagcatttta gatgccgcga tcacgggctt ctcgcaattc ccggaggatt
     1441 tcctgcaaac aatgatacaa ctgttgtaaa ggttctgtca ggtcacgctg tgagccgccc
     1501 tgggcaaggg cttgctccgc ttggtacgcc acgtcggcag cacggcgggc gccaaagtgc
     1561 cggaacgatc cgcccaaggt gtgggttgcg cgtcgtgctg tgggccagtc ttcttgagaa
     1621 acggcttttt ccagattttc ccactgtcgg ggcatttcct ggatgaacgt attgaccagg
     1681 gcacgcagca actcccgatc tcctcctgcc gcccgcaagg cgtgttgcca gtccacatga
     1741 gtgctctcgc cgggccttgt ttctaaggcg ctctccggcg agttcacttc gctggttggt
     1801 ggcgatggca gcgaattctc ttgaggagcc ggtgtatcgg ccgcagttgg tgtgggggaa
     1861 attgaaccgt ctggagatga cgtgctcatt tcgagcgttg gcagagcctg cgtgtcaccc
     1921 aggttgatgc cacggacctg gtgcaagaca tccgctattg tttggaagaa ttcctccacc
     1981 acgatgggct tggctacata gccatccatg ccggctttca aggcagcctc ccgatcgctt
     2041 tttagggcgt gggccgtcat ggcaataata ggagtccgcg gttgttggag gcgttcttcc
     2101 ctggcccgaa tctccttggt tgccgtcagg ccgtccatct ccggcatttg aatgtccatc
     2161 aggatgaggt cgaaagaccg gttgcggagg atttccaaag cctctttgcc attggaggcg
     2221 acggtcaccg tatgcccacg cttttccaac accccgatgg ccacctgttg attgaaagga
     2281 ctgtcctcca cgagcaaaat ctgcaagggg ggaagttctg gaaaactggc cgggatggta
     2341 gtctttgact cgggatggaa tgccagaccc aggatttcgg ctaggatgtt gtaaagctcc
     2401 gactgtttga ccggcttgcg gagaacaccg gaaacctcga agcgtcggaa aaactcgctg
     2461 tcggtcggtc ggtcttccga ggaaagcacg atgatcgcca gttgttcggc gccaaaacga
     2521 cgtcgcaact ggcggatcag cgccataccg tccatgccgg gcattcgcat gtcggtaata
     2581 acaacaagga agcggacctg tcctgcctca tccgtgacga gccgtaaggc ttcttcccca
     2641 gaggccgcta attccgactt caatccccag ttggcaatca tctcactgag gatgacccga
     2701 ttggttgcgt tgtcatcgac gacgaggatg ggtcggtcgg cgtattcccg caaatcgact
     2761 ggtaagccct tgggaagtgt ttcgctgggg cggcggaatc gggcggtgaa ggtaaacgtg
     2821 ctgcccaccc ctggctggct ctccaccgta atggtgcccc ccatcaagtt gacaaggcgt
     2881 ttgcagatcg ccagccccag gcctgtcccc ccatattttc gcgtcgtgct cttgtccgcc
     2941 tgctcgaaag cggcaaatat cacctgctgc ttttccgggg gaataccaat gcctgtgtcg
     3001 cggacggagc agcggaggac aacgtcgcta gtggtacatt catcgcacgt gacatccacg
     3061 acgacttcgc cctgctccgt gaattttatg gcattatcga caagattgat aaggatttgt
     3121 cttagccgaa cgggatcgcc gaccagctct tcggggacat ttggggcgac gcggcaggcc
     3181 agctcgagct gtttcttgtg ggcccgcaca gccagggcct tcatcgtgtc accgatcaac
     3241 tcgcgggcgt taaaatccac gctatccagt tcaatgcgtc ccgattcgat cttcgagaaa
     3301 tcaaggacgc tgttgatgag cgcgagtaga ttgtcaccag cctcacggat gatgcgaaga
     3361 tattcctttt gttcggcgtt aaggggtgtt tgctcaagga gttgtgccat gcccaggatg
     3421 gcgttcatcg gcgtgcgaat ctcgtggctc atgttagcca ggaaatcgct cttggcccga
     3481 ttggcctctt ctgccgcttg cttggccttt tccaaggctc tttggacggc aaccttttcg
     3541 gtcacatccc aaaacgccac ttgcacccca acaatgttcc ccgcatgatc ccgtacgggc
     3601 gatttcaaga cctggacgta ccggatttca ccatcctttt cgtactcttc gatgtcctca
     3661 aaaatctcgc cggtttccat gacacggcga tcgtcccggc ggtatttcga ggccaaatcg
     3721 ggtgggtaaa aatcaaagtc cgtcttgccg tagatctcct ctcgcgacct tcccacaaga
     3781 tgacagaatg cagtgttggc aaacgtaaag tgcccctcac gatccttgcg caagacgtga
     3841 agcgggaggc tttccacaag agacgagtac aaggcctcgg agtcgcgcaa gcctttttcc
     3901 actttctccc gctgctcaat ctcggcctcc aatcgccggt tcgtttcccg tagttcgtga
     3961 gtcttctccg ccaccaactg ttgaatacgg agcgagcgac caccaatgac gttgacgtaa
     4021 agggccaaca aaaacgagca taacgtgccc gcggccaaaa caccccaact tcctgatcct
     4081 cggcgcgaag caacgtaagt cgcggtcggt tgggtaatca cccgccagac gcgaccgggc
     4141 aaccgaatat cccaactcga cgtgggatac gtttgaagca agcgctccgt tcgggaggac
     4201 ggcctttccc aagcctccac cacatgttgc gaggttcctt ggaggtcaac caagcgaaag
     4261 ttaattccct cgaccgctgc ccagcgtttc aggggacgca gcaactcctg gacatcaaca
     4321 acacacacaa ggacaccgcc acattgggtg agccgttcaa acgggttctc cgggatatat
     4381 ccgccgaaaa aaaccggtga acagatggcg aggtattttc gtggggcaag gcctgccgcc
     4441 gaacttcggc tgtactgggt ggaaaacgtt tcctcttcgg attcgagaag acttgaggaa
     4501 ccaactcctg aatcggcttc aaaaacgacc tcatcgatgg cggggacacc ttccaaaaaa
     4561 tggacgtcca cctcgccgat cataattgtc tcctccagag cctccgccca ggaaggaatt
     4621 gatgcgatgt ctagccccac aaggcgatca aatgggggtg acggttcgat gaaaaggatc
     4681 gggaaatgct ggggtcgcgt ttggacaaag gtcctttgcc ccgcttcatc aagttcccag
     4741 ggttgaaagt tcgcaacgcc gtggcgatct gcacactctg ccagcgtttc aaatccatcg
     4801 ggataagtca ccggcgccca ggcggcgaac ttcaccacgg gaatattatc caagtgagac
     4861 cgacagaact cggcaaactc gtagcggtgg acatcttccg aagccgcgaa aaaggatcgc
     4921 aaaaactgca ataggttctg gctttgctga agtttgaact cgatgttctg gacctgtaac
     4981 tgcgattgat gactgcactg gctgacaatc cgcatggttt cccgttgggc tgtcacccgc
     5041 cagatgtagc ccgacacggc cagcgctatc gccagcacga ccaacgtggc aatccagacc
     5101 cgcgcccttg cccaccatga aacggccgta ggcggacttt cgaccgatct gtcattgaca
     5161 ccgttatgag acatcagaca agctcaatgg cgacgtagcg ccacaatgta ggcccagcca
     5221 gccttcgccg cggtctttaa aggacgtcct ccctactcct tcccagcacg cactcttgat
     5281 ttcctaaggt cccctcgggt tgaaa
//
LOCUS       DAOPTR010000113         5270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_12718_length_5270_cov_3.697987, whole genome shotgun sequence.
ACCESSION   DAOPTR010000113 DAOPTR010000000
VERSION     DAOPTR010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12718_length_5270_cov_3.697987"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2485
                     /locus_tag="PKI05_03385"
     CDS             <1..2485
                     /locus_tag="PKI05_03385"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOA51271.1"
                     /translation="EAPAAERVGIEIPGDGGAFTRQCCKMATGSGKTIVMAMAIAWHI
                     LNKVSNPQDARFSKNVLVVAPGLTVKKRLQVLELTADGNYYEAFDIVPSGLLDKLRQG
                     KVLVRNWHALAWETEEEIKKRKSVDKRGVKSDEAYTRDVLGEMANARNILVINDEAHH
                     AWRVNWEAEGKYLRQRDLKDSAEEATVWIGGLDRLHRARGILKCYDFSATPFTPSGKR
                     TSEEALFGWIVSDFGLNDAIESGLVKTPRVVVRDDAVPDAKTYKSRLYHIYNDPEVKN
                     DLNRKAEPEERLPDLVLNAYYLLGYDWRETLKAWQAAGHLTPPVMITVCNRTETAARV
                     KHAFATKQIHIDELCDTERILHIDSKVLAEAEQKEEAFTPVRAVGESESDDTEEEDTP
                     AERKLTKAEQAELLRQMVDTVGKKGQLGEKIQHVISVGMLSEGWDAKTVTHIMGLRAF
                     TSQLLCEQVVGRGLRRTSYEVNPETGLFEPEYVNIFGVPFTFLPHEGGKDGPPPPPTP
                     KTAVEPDPAKAQYEIRWPNVVRIECVLQPKLEVDWTNVHPLELDAARTMRVAELAPII
                     EGKPDVTKIERIELERLAREFRTQRIIFETARDVFDRIKPSWRGTREVLLAQLVKIVE
                     RFIASDRITIKPPLFYQDELRRRLIITLNMTRVVEHVLQAVRQENSDRLVPIFDCDHP
                     IRSTAEMRTWYTGKPCERTKKSHINVCVYDSTWEASAGYVLDNSDEVAAWVKNDHLGF
                     EIFYLYQGVVRKYRPDFLVRLINGDHLVLEIKGQNTEQDEQKRKYLEEWCQAVTAHGG
                     FGRWQCDVVRSPGEIHQVLARALANARSN"
     gene            2935..3906
                     /locus_tag="PKI05_03390"
     CDS             2935..3906
                     /locus_tag="PKI05_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51272.1"
                     /translation="MSIKLDFGPLDAAVQEFSEAAEAFEKLWMEDDRRPACVKPKPKV
                     SDKVVRQAIVRLLKAARHLLDAVEAMPPNVRQALVHPPPARVGVCCPPLPLPQQAREV
                     IAFAEQWLPKFTDPRFWVNADFEYDFYFYVGQLLLTVWYELTKTLECYQRLKEQIEVD
                     VLLDRADTWLGNVALQDAERAAPVADETTNHDTRPAEVKESVPHGTRQGGVGRSKRST
                     EKGEARLKITAALFHHHQYDGDSVLNDDPIGVNELHRLAGVSKGMVSTFFKKEFCPQN
                     QSDGHARYRALCRDRRRLIAWLKTLDPNEKIRGHQLLNSDIPSRPED"
     gene            4179..4424
                     /locus_tag="PKI05_03395"
     CDS             4179..4424
                     /locus_tag="PKI05_03395"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOA51273.1"
                     /translation="MNTPEPLAYRLKDAARILGLSTRTVWQLAHDGQIPCVRVGTGRR
                     KTLLFPADALKAWLLERAKEDRAGDPGNTTEDGLNDR"
     gene            4414..>5270
                     /locus_tag="PKI05_03400"
     CDS             4414..>5270
                     /locus_tag="PKI05_03400"
                     /inference="COORDINATES: protein motif:HMM:NF013930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51274.1"
                     /translation="MTVDEVVALLERHGCHPRRAGAGWVARCPAHDDRKPSLSVSPGR
                     DGQILVHCFSGCTLESILAALGLRWSDLFPDDPLRRRDHHPKRVFTLGPWRSTGKPDF
                     VRYIEACRIPRGHPRLEAIAHSLGVSPESLFRLQVGWDHQRQAVTFPMSDALGHIVGI
                     RFRRSDGSKLSLRGGSEGLFLPIDLQPRPGQLLITEGPTDCAAALTLGFQAIGRPSCR
                     GAQRQAVELVRRLRPAQVVIVADNDTPGLEGGRKLARALILHVGHVRLVQPPPGVKDL
                     RAWVRQGCTP"
BASE COUNT         1147 a         1523 c         1571 g         1029 t
ORIGIN      
        1 cgaggccccg gctgccgagc gggtgggcat cgaaattccc ggcgatggcg gggcgtttac
       61 gcggcaatgc tgcaaaatgg ccaccggctc ggggaagacc atcgtgatgg ccatggccat
      121 tgcctggcat atccttaaca aggtatcaaa cccgcaggat gcccgctttt cgaagaacgt
      181 cctcgttgtt gcgccgggct tgacggtgaa gaagcggctt caggttctcg aattgaccgc
      241 cgacggcaac tattacgagg ccttcgacat tgtcccctct ggcctgctcg ataaattacg
      301 gcagggcaag gtcctggtcc gtaactggca cgccttggca tgggaaaccg aagaagagat
      361 caagaagcgc aaaagcgtgg acaagcgcgg cgtcaagagt gacgaagcct acacgcgtga
      421 cgtgctcggc gaaatggcca atgcccgcaa tattctggtg atcaatgatg aggcacacca
      481 tgcctggcgc gtaaactggg aggctgaagg caagtatttg cggcagcgtg atctcaagga
      541 tagcgccgag gaggcaacgg tctggatcgg tggcctggac cggttgcacc gcgcgcgtgg
      601 cattctcaaa tgctacgact tctcagccac tccatttact ccgtcgggaa agcggaccag
      661 tgaagaagcc ctctttggct ggatcgttag tgactttggg ttgaacgatg ctatcgagtc
      721 gggcctggtt aagacgccac gagtggtggt gcgcgatgat gctgtgccag atgccaagac
      781 ctataaatcg cgcctctatc atatctacaa cgacccggag gtgaaaaacg acctgaaccg
      841 aaaagcggag ccggaggaac gactgcccga ccttgtgctg aacgcctact atctgcttgg
      901 ttacgattgg cgggagacgc tgaaggcatg gcaggcggcc ggccatctta ccccgccggt
      961 gatgatcacc gtctgcaatc gcacggagac ggcagcccgg gtaaagcacg ccttcgcgac
     1021 caaacagatt cacatcgacg agctatgcga tacggagcgc atcttgcata ttgactccaa
     1081 ggtgctggcc gaggcggaac agaaggagga agcctttacc cctgtccgcg cggttgggga
     1141 gagcgagtcc gatgatacag aagaggaaga caccccggca gagcgcaagc tgaccaaggc
     1201 tgagcaagcc gaactcctcc gccagatggt ggataccgtc ggtaaaaagg ggcaacttgg
     1261 cgagaagatt cagcatgtca tctcggtcgg catgctttcc gaaggctggg acgccaagac
     1321 ggtaactcac attatggggt tgagagcctt tacgtctcag cttctctgcg agcaggtggt
     1381 ggggcgcggg ttgcggcgta cctcctacga ggtcaatccg gagacgggtc tgttcgagcc
     1441 ggaatacgtc aacatctttg gcgtgccctt tacatttcta ccgcacgagg gaggaaagga
     1501 tggcccgcca ccgccgccga caccgaagac ggccgtggag cctgacccag ctaaggccca
     1561 gtatgagatt cgctggccta acgtagtgcg gatcgagtgc gtgctccagc cgaagttaga
     1621 agtggattgg accaatgtcc accccttgga actggatgcc gcccgaacga tgcgggtagc
     1681 cgaactggca cctattatcg aggggaagcc cgacgttacc aagatcgaac gcattgagct
     1741 ggagcggctg gcccgcgagt tccgcaccca gcgaatcatc ttcgagaccg cacgcgacgt
     1801 gttcgaccgg atcaaaccct cttggcgggg cacccgcgag gttctgctgg cccagttggt
     1861 gaagatcgtc gagcgattca tcgcttctga ccggatcacg attaagccgc ccttgtttta
     1921 ccaagatgaa ctgcgccggc ggcttattat tacgttgaac atgacccgtg tcgtcgagca
     1981 cgtcttgcag gctgtgaggc aggagaatag cgatcgcttg gtgcctatct tcgactgcga
     2041 ccaccctatt cgctccactg ctgagatgcg cacctggtac acgggcaagc cctgcgaacg
     2101 cacgaagaaa tcacacatca acgtctgcgt gtatgacagc acctgggaag cctcggcagg
     2161 ttatgtgctt gataacagcg atgaggtcgc cgcctgggtg aaaaacgacc acctcggctt
     2221 tgaaattttt tatctttacc aaggggtggt gcgaaagtac cgtcctgatt ttttggtccg
     2281 ccttataaac ggcgatcatc tcgtcttgga aattaaaggc caaaatacgg aacaggatga
     2341 gcagaagcgc aagtatctgg aggaatggtg ccaggccgtc actgcccatg gcggctttgg
     2401 ccggtggcaa tgtgatgtgg tgaggtctcc tggtgaaata caccaggtgc ttgcgcgtgc
     2461 cctggctaac gcgagatcga actgattcta acgcaagatc gaactgatcc tcagtgaagc
     2521 tcacgccggc gcttggaagg tcattgaccg gtccgcgtca cttccgcgac attcggggta
     2581 cgcgaagcca agcgagctgc tcaattgctc gaagccgctt cccgaatggc ggtttactgg
     2641 catggtgacg ccgaaacgaa cggggatttg ctccggcggg caatcttcgc aaggcgttcc
     2701 caaggcagtg tgaccggcaa gcggcaaagc ttgcgaaggc aattccgcag aaccacgggg
     2761 gccaggttgg acatgggttg tccaacctga cgcggcgacg gcctttgagg ggcagcgtgt
     2821 cagcgtgcta taatcggcac ctaatcgcac actacggaat gcatcctgaa cactacgtaa
     2881 gggcaacgct gcccgccggt tttttggccg catctgcatc gcggagcgag acccgtgtct
     2941 atcaaacttg attttggccc tttggacgcg gcagtccagg agttttccga agccgcggaa
     3001 gcctttgaga agctttggat ggaagatgac cggcggcccg cgtgcgtgaa acccaagcca
     3061 aaggtcagtg acaaggtggt gcgacaggca attgtccgct tgctgaaagc cgcccgtcac
     3121 ctgctcgatg ccgtcgaagc gatgccgcca aacgttcgtc aggccctggt gcacccgcca
     3181 cctgcacgag taggggtttg ctgcccacca ttaccgctac ctcagcaggc cagagaagtc
     3241 attgcttttg ccgaacaatg gcttcccaag tttaccgacc cgcgcttttg ggtgaacgca
     3301 gatttcgagt atgatttcta cttctacgtg gggcagttgc tgctgactgt ttggtacgaa
     3361 ctgacgaaaa cgctcgagtg ctatcagcga ctcaaagagc aaatcgaagt cgatgtgctt
     3421 ttggatcggg cggatacgtg gctggggaac gtagcacttc aggacgcgga aagagccgcc
     3481 ccggttgccg acgagaccac caatcacgac acgcgaccag ccgaggtgaa ggagtccgtc
     3541 ccgcacggga cgcggcaagg cggcgtggga cggagcaagc gcagtaccga aaaaggcgaa
     3601 gcccgactta agatcaccgc ggccttgttc caccatcatc agtacgacgg cgacagcgtc
     3661 ttgaacgatg accccatcgg cgtcaatgag ctccatagat tagccggcgt atccaagggg
     3721 atggtttcga cattcttcaa aaaagagttt tgccctcaga accagagcga tggccacgcc
     3781 cgttatcgcg ccctctgccg tgatcgccgg aggttaattg cgtggctgaa aacgctcgac
     3841 cccaacgaga aaatccgcgg ccatcagctt ctcaacagcg acatcccctc acgcccagaa
     3901 gactgatcaa cgggcgtttt ccctgttcac tattcaatcg ttcaattccc cgttcactgt
     3961 tcaatcgttc aattccgtgt tcactgttca atcgttcaat acgaaatgtt gaacaccgga
     4021 acaattttct tgtccacgta accccttaca gataaagacg ttacgtcgcc acgtcggggg
     4081 tgttccacgc tcccatgttg aacactgcgg tcctatgtag agggccgcac ggtgcagccc
     4141 acagacgtaa accccctgaa accccaggag acgtaacgat gaacacgcca gaacccttag
     4201 cctaccgtct taaggacgcg gcccgaatct tggggctttc cacccgcacg gtgtggcaac
     4261 tagcccacga tggtcaaatc ccgtgtgtac gtgttggcac gggccgacga aaaacgctct
     4321 tattccctgc cgacgccctg aaggcatggc tccttgaacg ggccaaagag gaccgcgccg
     4381 gcgacccggg caacaccacc gaggacggcc tcaatgaccg ttgacgaggt ggttgcacta
     4441 ctggagcggc acggatgcca cccacgccgg gccggcgccg gctgggtggc ccgttgtcca
     4501 gcccacgacg accgcaaacc gagtttgtcg gtgtcgccag gccgcgatgg acaaatcctt
     4561 gtgcattgct tctccggatg cacactggaa agcatccttg ccgccttggg gctgcggtgg
     4621 agcgaccttt tcccggatga cccattacgc cggcgcgatc accaccccaa gcgggttttc
     4681 accctagggc catggcgatc gacgggaaag cctgattttg tccggtacat cgaggcttgc
     4741 cggattcccc gcgggcatcc ccggcttgag gccatcgccc atagcctagg ggtttcgcct
     4801 gaaagcctgt tccgactgca agtgggctgg gaccaccagc ggcaagcggt gactttccct
     4861 atgtcggacg ccctggggca catcgtcggc atccggtttc gtcgttctga tggttccaag
     4921 ctgtccttac gaggcggaag cgaagggctt ttcctaccga tcgacctgca accccgaccg
     4981 ggacagttac tcatcacaga aggcccgacg gactgcgcgg ccgccctgac gctgggcttc
     5041 caagcgattg gccgtccctc atgtcgtggc gcccaaaggc aagccgtgga gctggtccga
     5101 cggcttaggc cggcgcaagt ggtcatcgtg gccgacaacg acaccccggg tctggaaggg
     5161 ggcagaaagc tggcccgggc gctgatcctc cacgtcgggc atgttcggct tgttcaaccc
     5221 ccgcctgggg tgaaagacct ccgcgcgtgg gtccgtcagg gttgcacgcc
//
LOCUS       DAOPTR010000114         5269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_12722_length_5269_cov_3.375527, whole genome shotgun sequence.
ACCESSION   DAOPTR010000114 DAOPTR010000000
VERSION     DAOPTR010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12722_length_5269_cov_3.375527"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PKI05_03405"
     CDS             complement(<1..425)
                     /locus_tag="PKI05_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOA51275.1"
                     /translation="MDVQRCGTTWVVHAPAKVNLFFEVHGRREDGYHLVETLVCPISL
                     YDTLAFEENPDDDVVLSVQFITGGASLPECGFDTVPDGPSNLVYRAAELVKKETGTRK
                     GAKIRLWKRIPTGAGLGGGSSDAAATLLGLCRLWGLTPG"
     gene            813..2096
                     /locus_tag="PKI05_03410"
     CDS             813..2096
                     /locus_tag="PKI05_03410"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007333174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOA51276.1"
                     /translation="MSATAVIGLQWGDEAKGKIVDLLTEDHEIVVRYQGGANAGHTVV
                     RQGQTFKLSLIPSGIFQPNVTCVIASGVVVNLKTLLDEIALLRSRGVSISNNLLLSDR
                     AHVIFPWHLEEDRILNECPSGEAIGTTLRGIGPCYRDKVGRSHAIRVGDLFESGFKER
                     IEAIVQAKARFLVPLGVSPDSPSLDAAAIFREYSGYAEAIRPFVCDTTEFLLTAAERE
                     RRILLEGAQGALLDVDHGTFPYVTSSNSSGAGISAGSGIPAKYIKKVLGVVKAYTTRV
                     GGGPFPTEQDNPIGQTIRDRGNEYGTVTRRPRRCGWFDAVAVRYTARLSGVDAIAVML
                     LDVLSTLPELKICVAYELDGERITHFPSHVEQLRRVKPVYETLPGWSEEIQHVRRLQD
                     LPKAARAYLDRLAELLRCPIEMVSVGPDREQTIIC"
     gene            2233..3039
                     /locus_tag="PKI05_03415"
     CDS             2233..3039
                     /locus_tag="PKI05_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOA51277.1"
                     /translation="MSDSRFEATLDSQNLMCGELLKTSDALDVPPEKRPRHIAAIMDG
                     NGRWAQRRGLPRIEGHRHGVAAVRRTVEECVRLGIEQLTLYCLSSENWKRPKFELDFL
                     LRLLEQYIVEERGTFTENNVRVQVIGRREGIPPEVQRELDKTLQLTANNTGLCLCLAV
                     NYGGRVEILDAVRKIAEKVAVGQLSINDIDEATFSDHLYTAGMPDPDLLIRTAGEQRV
                     SNFLLWQISYAELWITDVCWPDFNEELLHQAIRDYSRRVRRFGGLVRSPS"
     gene            complement(3110..4507)
                     /locus_tag="PKI05_03420"
     CDS             complement(3110..4507)
                     /locus_tag="PKI05_03420"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOA51278.1"
                     /translation="MKLQFLGANRQVTGSQYYLQAGKTRLLIDCGMFQERPYLDRNWD
                     PLPVPPREIDALLLTHAHLDHSGLIPKLVREGFRGKILATSASVDVAEIVLRDSAHIQ
                     VEDAAYKRKRHRKEGRTGKHPEIPLYDVNDVEEALPYFRAVPYYQPIELDGVTIRFLD
                     AGHILGSAFLVVDAKENGRERRIVFSGDLGSPDRPILRDPDMIHEADYLVIESTYGDR
                     DHNHGPSVEDQLVKVVRETSARGGHVVIPVFAVERAQELLFYFSRLYRSGRLPPLPIF
                     LDSPMAVRITEVFHRHRECFDQETQNLIDNDQSPFNFPNLRLVTTVEESKQINTYNVP
                     SVIMATSGMCTAGRIKFHLRNNIVRPESTILFVGYQSRGTLGRQILDKNPYVRIHGKE
                     WPTRARIEQIQGLSGHADRKGLLSWVSQVKSPVKSVFVTHGEENASLSFAEMIRTQLN
                     WRTSVPQYMDEVELD"
     gene            complement(4711..>5269)
                     /locus_tag="PKI05_03425"
     CDS             complement(4711..>5269)
                     /locus_tag="PKI05_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51279.1"
                     /translation="APAGKKIPILSGEWGYSAVWRNYDETRQGKYLPRQWLTNLAAGI
                     PVCIWYGWHDDGPDPKEPEHHFGMVAYPYHEGRNPVYDPKPAYLAAQTLCTLLAGYRF
                     VERLPVKAEKAELCHALRFEKGGKSAVVIWYQQDGQTTLVQVALPPGLYRVIDYLGKD
                     LGTKNVVAASTELEITDAPLYLVQE"
BASE COUNT         1167 a         1442 c         1492 g         1168 t
ORIGIN      
        1 atcccgggag ttaatcccca gagacggcac aaacccaaca acgtggctgc cgcgtcggaa
       61 gaccctcccc ccaaccctgc gcctgtcgga atccgcttcc acaaacggat tttagcacct
      121 ttgcgggtgc cggtttcttt ctttaccagt tcggcggccc gataaaccaa gttgctcggg
      181 ccgtcgggaa ccgtgtcaaa accacactcc gggagcgacg cccctcccgt gataaattgt
      241 acactgagga ccacgtcgtc atccggattt tcctcaaaag cgagggtatc gtagaggctg
      301 ataggacaaa ccagcgtctc gacgagatgg tatccgtctt cgcggcggcc gtgcacctcg
      361 aagaaaaggt tcacttttgc aggggcatga acgacccaag ttgtgccgca acgctggaca
      421 tccattccgt gtcggccccg gctttagatt tcccatccgc ttgccaacgc gatcccccca
      481 gcaatcggga ttttaatggt tttgccgtcc ctgtcaatat cgctgcgggt gatgaacacg
      541 tgtgtggacg aattgagcct tctccccggt ccgaagttcc gagcgtgcgg ttccttgacg
      601 gttcgaactg atggcgatta gactgcaact ctggagctgt tctagacgaa actggccgtg
      661 gcacggaggg gaccagcggt tctagccgcg caaaccacaa acgcgcgggc ttccgctcgc
      721 gcttgttttc ggaatttcgg cctttgggaa cggcataggt tggacgacgg actttcgtga
      781 tcgtgttgac taacaagaga aaggtgaatc tcgtgagcgc caccgctgtc attggtctgc
      841 aatggggcga cgaagcaaaa gggaagatcg tcgatctcct taccgaagac cacgaaattg
      901 tggttcggta tcagggcggg gccaacgccg gtcataccgt cgtgagacaa ggtcagactt
      961 ttaagctgtc cctgatccct agcggaatct ttcaacccaa cgtgacgtgt gtcatcgcgt
     1021 ccggcgttgt cgtcaatttg aagacgcttc tcgatgagat tgccctgctc cgctcaagag
     1081 gggtctccat ctcgaacaat cttcttttga gtgatcgcgc ccatgtcatc ttcccatggc
     1141 acttggagga ggatcgtatc ctcaacgagt gcccaagcgg tgaggctatt ggcacgacgc
     1201 ttcgaggcat tgggccatgt taccgagaca aggtgggacg gtcgcatgcc attagggtag
     1261 gggacctgtt cgagtctggt ttcaaagagc ggattgaggc catcgtgcag gccaaggccc
     1321 ggtttctggt ccccctgggg gtttcgccag atagcccgtc cctcgatgcg gccgccatct
     1381 tccgtgagta ctccggttac gccgaggcca tccgaccttt tgtgtgcgac acgacggagt
     1441 ttttgttgac tgccgcggaa cgtgagcggc ggatcctcct agagggggca caaggagccc
     1501 tccttgatgt tgaccatgga acctttccct atgtgacgag cagcaatagc tccggtgcgg
     1561 ggatttcggc aggctcgggg attccggcca agtatataaa aaaggttttg ggggtagtca
     1621 aagcatatac aacacgtgtc ggcggtgggc ctttcccgac agagcaggac aaccctatcg
     1681 gtcagaccat ccgggatcgg ggaaacgaat acggaacggt cacccgccgc ccgaggcgct
     1741 gcggctggtt cgatgccgtt gccgtgcgtt acacggctcg actgagtggc gttgacgcta
     1801 ttgccgtgat gctcctggat gttctgagca cgttgcccga gctaaagatt tgcgtggctt
     1861 acgaattgga cggggagcgg attacccatt tccccagcca tgtcgagcaa ctgcgacgcg
     1921 tgaagcctgt gtatgagaca ctccccggat ggtccgagga gatccagcac gtccgccgct
     1981 tgcaggactt gcccaaggca gcgcgggcat atctcgatcg cctggcagag ttactacggt
     2041 gtcccattga gatggtttct gtgggccccg accgcgagca aacgatcatt tgctaaacgg
     2101 gcttcgtcct tcagagtgtg tgcttcaagc ctccacaccc gacggctatt ttcaagggaa
     2161 atgacaggac ttgtggcaaa ccccacatgg tcgccgggcg aatgtttttg ctaaactgga
     2221 tgttagggca agatgtcaga ttcccgattt gaggcgacgc ttgattccca aaacctgatg
     2281 tgtggagagc tgctaaaaac gtccgacgct ttagatgtcc caccggagaa gcggccccgg
     2341 catatcgctg ccattatgga cggcaacggt cgctgggcgc agcggcgggg gttgcctcgc
     2401 attgagggac accgtcacgg tgtggcggct gttcgccgga cggtcgaaga gtgcgtccga
     2461 ttgggaattg aacagctcac tttgtattgc ctctccagcg agaattggaa gcggcccaag
     2521 ttcgagctgg atttcctttt gcgattactg gagcagtaca tcgttgagga gcgggggacg
     2581 ttcacagaaa acaacgtgcg ggtgcaagtg atcggacgga gagaaggtat cccgcccgaa
     2641 gtgcagcgcg aacttgataa gaccttgcag ttgaccgcca acaacacggg attgtgcttg
     2701 tgtctggccg tcaattacgg cggaagggtg gaaatcctgg atgccgttcg caagatcgcg
     2761 gagaaagtgg ctgtggggca attatccatt aacgacattg acgaggcgac gttctccgac
     2821 cacctctaca cggcgggcat gccggacccc gatttgctta ttcgcacggc tggcgagcag
     2881 cgagtgagta actttttgct ctggcaaatt agctacgccg agttgtggat cacggatgtc
     2941 tgctggccag acttcaacga ggaattgcta catcaagcca tccgggatta ttcccggcgg
     3001 gttcggcgat ttggtggcct ggtccgatcg ccttcgtgat tgccagggtt cttatcacgc
     3061 aaagctcaca gccgttcggc ggaaatgtcg tgcgcttcag cccggtgggt caatctaatt
     3121 ccacttcatc catgtactga ggaacgctcg tccgccagtt aagctgggtg cggatcattt
     3181 ctgcgaagct taaggaggcg ttctcttcgc cgtgggtcac aaacaccgac ttcaccgggg
     3241 atttgacttg ggaaacccaa ctgagaagcc ctttacgatc cgcatgtccc gataggccct
     3301 ggatttgttc gatcctcgct cgggtgggcc attctttccc gtgaatacga acatacggat
     3361 tcttgtcgag gatttggcgt cccaacgtcc cgcgagattg ataacccaca aaaagaattg
     3421 tcgattccgg tcgcacaata ttgtttctca aatggaactt gatccgccca gcagtgcaca
     3481 tcccggaagt tgccatgatc acggacggga cattgtaagt attgatctgt ttggattctt
     3541 caacggtcgt caccagtcgc aagttaggaa aattgaaggg cgattgatca ttgtcgatga
     3601 ggttttgcgt ctcttggtcg aaacactcac ggtgacgatg gaagacctca gtgattcgca
     3661 cggccatcgg actgtccagg aagatgggca agggtggaag gcgtcccgac cgatataggc
     3721 ggctgaaata gaagagcaac tcttgagccc gctcaaccgc aaaaacggga ataaccacgt
     3781 gaccgccccg ggcagatgtt tcccgaacga ctttgacgag ctgatcctcc acacttggac
     3841 catgattgtg gtcacggtca ccgtacgtcg attcgatgac gaggtagtcg gcctcgtgga
     3901 tcatgtcagg atcacgaagg atgggacggt ccgggctgcc caggtcgccc gaaaaaacaa
     3961 tccgtcgctc ccgaccattc tcttttgcat ccacaaccag aaacgccgag cccaaaatgt
     4021 gaccggcatc aagaaagcgg atggttaccc catcaagctc gataggctga tagtatggga
     4081 cggcacgaaa atagggcaag gcttcctcaa catcattcac atcgtagagg gggatttccg
     4141 gatgcttgcc cgtccgcccc tccttgcggt gccgcttccg cttgtaagcg gcgtcttcca
     4201 cctgaatgtg ggccgaatcc cgaaggacaa tttctgccac atcgacggag gcagatgtgg
     4261 caaggatctt cccgcggaaa ccctctcgaa ccagcttggg gatcaagcca ctgtgatcaa
     4321 gatgggcatg ggtcaaaagg agggcgtcga tttcccgcgg tggcacgggt aaagggtccc
     4381 aattacggtc caggtacgga cgctcctgga acataccgca atcaataaga agccgggttt
     4441 tacccgcctg caaatagtat tgggaacccg taacctgccg attggcaccc agaaactgaa
     4501 gtttcactgc tcatcctcgt cgaatgccaa atgtgcgacg ctcaagttac ccccataacc
     4561 catcggatcg ggaagaccgc aatgtaagac caacttgtag gaaaggcaca atcgataccc
     4621 ttttttcaag cggccctact tataggacgt tggcatagcg aaaaatgttt gtttcttttg
     4681 acagaaacat caagaccagt ctgtccgcga ttattcctgg acaagataga ggggcgcgtc
     4741 ggtgatctca agctcggtcg aggccgcaac cacgttcttg gtgcccaggt cttttccaag
     4801 gtaatctatg acgcgataaa ggcccggtgg cagagcgacc tggaccaaag tcgtctggcc
     4861 atcttgttga taccagatta ccacggcgga cttcccaccc ttttcgaacc tcagggcatg
     4921 acacaattcg gccttctcgg cctttaccgg cagacgttcg acaaaccgat atcctgccaa
     4981 taacgtgcat agcgtctggg cagccaagta cgcaggtttc ggatcataaa ccggatttcg
     5041 tccctcatga tagggataag ccaccatgcc aaaatgatgc tcaggctcct tggggtccgg
     5101 cccgtcgtca tgccaaccgt accaaatgca aacaggaata ccggccgcca ggttagtcaa
     5161 ccattgtcga ggaagatact tcccttggcg ggtttcgtcg tagttccgcc acaccgccga
     5221 atagccccat tccccagaga ggatcggtat ctttttaccc gccggggcg
//
LOCUS       DAOPTR010000115         5234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_12825_length_5234_cov_4.312222, whole genome shotgun sequence.
ACCESSION   DAOPTR010000115 DAOPTR010000000
VERSION     DAOPTR010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12825_length_5234_cov_4.312222"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1515
                     /locus_tag="PKI05_03430"
     CDS             <1..1515
                     /locus_tag="PKI05_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51280.1"
                     /translation="LDTLQGYTAPFCTNIHEPKPGEALSIWACLIRGEPLPPGKEWLI
                     RGTAVYGGGSVIDVPVSVAGDSFYYVPTHELNAGCAVIAYRMQRPGESGHPVPADLPR
                     IGSEEEEKVRAMPWDWDVLGTPRLRKAIDELRPPVPGTWSAPLTEATTAVAKIPDAEL
                     DEIIWEVPAIPSTSSSRATSLRAQLSAAVTELISRDWRPLVFPAGKHPIEAYRFFAEP
                     TETLLTLARAYPHLDPGLQKRVAEKVAAMAAPGGPLDGPTGTRTFDPNVGEVRSAYSV
                     PPENLWRVTDDILRTSLARLYPFWLWAYMTGDWSHLEKHWGSIRDLVAQSPNDPKEDC
                     CNGHIAGLIAYCRIARHLGDNEAVRLGVNAARQAMRDRVAFEWAHPSGAVLSEVPVQR
                     QVFARWRNLTPEVARLCSKYAGDLQRQLIERYVDYHRPTWWLAWNVELAWRNECPFSL
                     PTMSLEIFAAKAMILGEPAERLCQYLDIPWCKADLYYIQKLVWCIEAEAGTTSQ"
     gene            2448..2609
                     /locus_tag="PKI05_03435"
     CDS             2448..2609
                     /locus_tag="PKI05_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51281.1"
                     /translation="MDKSVAGGRCTQAGLRNWTRRGAEAVLWLRAVQFDDFGLLRDAQ
                     LRLALINPP"
     gene            2932..3198
                     /locus_tag="PKI05_03440"
     CDS             2932..3198
                     /locus_tag="PKI05_03440"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOA51282.1"
                     /translation="MTPEVGWVGIEEVGAHPKVAKQSIYRWVVSKGFPAHRVGRLLRF
                     RLSEVDEWVTSSSGGDFASAPSGKTTESRPAGRKTNVKKKSRHG"
     gene            3191..>5234
                     /locus_tag="PKI05_03445"
     CDS             3191..>5234
                     /locus_tag="PKI05_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOA51283.1"
                     /translation="MAESRFSTQIGRQISLPGHFDVPVILEDVRALGGDGSGGYECRV
                     RLPDGTLEEAVISPAEAATLFSTDRESPTAITPADAEQLRLLIESARIRLAYAHDRQF
                     AVSLSGIRTLPHQIEAVYQAMLPQPRLRFLLADDPGAGKTIMAGLLIKELKLREAIER
                     ILILCPAPLTIQWQDEMLRWFGESFDIIFSAVDQQQLTNPWQRCSQVISSIDYAKQND
                     VRERVWQQRWDLVVIDEAHKCSAYTKSRTNRSDEVAETKRYQLAKRLSETADHVLLLT
                     ATPHHGDEDKFSHFLRLIDPDLFPEPHRLGNQATSIRKDVFKLGKDSPWCLRRLKEDL
                     RDLNGKRLFPDRHARTVTFRLNSEEYALYKSVTAYINEFIPGAQASSAGGQRRSAAAL
                     TRTVLQRRLVSSTCAIHESLKRRLKKQQDLLDELEGLSPAQRAKRLAALQKRLPDAEQ
                     EEDDLDDEVRDRLVDEYTAALELEQLRAEISALKELVEQARRVQDLERDSKLAALKKC
                     LQEAQFAELRDGRGKLLIFTEHRDTLTYVREHLERWGFTTCEIHGGMNPHERKRAQEQ
                     FRTTAQVCVVTEAAGEGINLQFCHLMINYDMPWNPTRLEQRLGRIHRIGQDRDVYAFN
                     FVATESEDGQPIVEGRILHRLLEKLDTMKEALKGRVFDVIGEVLSLNDVNLPDMLRE"
BASE COUNT         1066 a         1551 c         1609 g         1008 t
ORIGIN      
        1 cttgatacgc ttcagggata tacggccccg ttctgcacga atatccacga gccaaagccg
       61 ggcgaggccc tgagcatctg ggcttgtctg atccgcgggg aaccgctccc acccggtaag
      121 gaatggctta tccgcggcac ggcggtctat ggtggaggct cggtcatcga cgtgccagtt
      181 tccgtcgctg gcgatagctt ctattacgtt ccgacacatg agctgaacgc tggctgcgcg
      241 gtgatagctt atcgaatgca gcggccagga gagagtggtc accccgtgcc agccgacttg
      301 cccaggatcg gctccgaaga ggaagaaaaa gtccgcgcaa tgccctggga ctgggacgta
      361 ctggggacac cacgattgcg caaggcgatc gatgagctaa ggcccccggt gccggggacg
      421 tggtcagcgc cgcttacaga ggccactacc gctgtggcaa agattcccga tgcagagctc
      481 gatgagatca tctgggaagt cccggccatc ccaagcacgt catcatcgcg ggcgacatct
      541 ctccgggcgc agctttcggc cgcagttaca gaactcatca gccgagattg gcgcccgtta
      601 gtcttcccag ccggtaagca tccgattgag gcttaccgct tctttgccga acctaccgaa
      661 acccttttga cgctggcccg cgcttatccg catctggacc cgggattgca gaagcgcgtc
      721 gcggaaaagg tagcggccat ggcggccccc ggtggtcctc tggatggccc cacgggaacg
      781 cggacgtttg accccaatgt cggcgaagtc cgctcggcct actccgtccc gcctgagaac
      841 ctctggcggg tgaccgatga catcctccgc acgtccctgg cgaggctcta tcctttttgg
      901 ctgtgggctt acatgactgg cgactggtcc catctggaaa aacattgggg ttctatcaga
      961 gacctggtgg cccagtcccc caacgatcct aaagaggatt gttgcaatgg gcacatcgcg
     1021 ggattgatcg cctattgtcg cattgcccgt catctcggtg ataacgaggc ggttcgtctt
     1081 ggtgtgaacg cggcccgcca agccatgcgt gaccgcgtgg ccttcgagtg ggcccatccg
     1141 agcggtgctg tcttgtcgga ggtccccgtg cagcggcagg tctttgcccg ctggcgaaac
     1201 cttacgccag aagtcgcgcg gctctgctcc aaatacgctg gcgatttaca gcggcagttg
     1261 atcgaacgat atgtagatta ccaccgaccg acgtggtggc tggcctggaa tgtagagttg
     1321 gcttggcgga acgaatgccc gttttcgctc ccaacgatgt cgctagaaat cttcgcggca
     1381 aaggccatga tccttggtga accggcggag cggctttgcc agtacctgga cattccttgg
     1441 tgcaaggctg acctttacta catccaaaag ctggtttggt gcatcgaggc cgaggccggc
     1501 acgacaagcc agtaagctgg attcaggcca cgcattagcc accgtgtgca agtcgcattt
     1561 cgatgggccg gggatagcac gctgctcctc agattgtctt ccgtggggag actgcgtcgg
     1621 caaaccctct ccgcaagacg gacaagatca ctaacgagaa gaggagcacc ttcgtttctt
     1681 gcgggtcata cccattttgc aagtcagcgt aaggtacttg gaacccatcc cataatctcc
     1741 cccgttcggg ggggttcgaa ctgctgccgg ttttctcaat tggattgcga cgttcgctag
     1801 ccctcacccc aaccctctcc cggagggaga gggggacttt ttgagatgga ttcttagtag
     1861 cacgcccgga gggattcgaa cccccaacca gcggattaga aatcctgcta tcatttgtat
     1921 taagtctcgt cagaaacact acttacgtca cacatgaaat cggcgcaacg cacaagacaa
     1981 cgcagtgttt tgcacggcaa caactgcaac tgggacggga acagctgcga cgaggatgag
     2041 ggaaatggct gtccggcgat tcggaacccc acacgagaaa tccgaagagc aagaagatct
     2101 gatctaggtt ctcgggttgg ggaccctctg taggttctcg ggttggggac cctctatagg
     2161 ttctcgggtt gaagaccctc tataggttct cgggttgaag accctctgta ggttctcggg
     2221 ttggagaccc tctgaaatag agctttgtgg cccgtcaggc ggtggcggaa cgacgcccct
     2281 agcggcgcgg cgtgaagcat ggcgagcgct gggcgtgtct gtgaagaaca ccgccccgtg
     2341 catgtgaaga acagcgcccc gtgtctgtga agaacaacgc ctagccaacg gactacccgc
     2401 gatgtcgccg ggagggccta ccggtgggca gcggagcggt ggagtcgatg gacaagagcg
     2461 tggcgggcgg acggtgcaca caagccgggt tgcggaattg gacccgccgt ggagcggaag
     2521 cggtgctgtg gcttcgggcc gtccagttcg acgacttcgg cctcttgcgg gatgcccagc
     2581 ttcgcctggc tctcataaat ccgccataat ctgcagctac acgtttgaca gtgcgacctg
     2641 cgacttgtag actctcttga aacgtcgtgg tcagctggag tttacccagt gtcgaaatag
     2701 ttgagggcga ggattttggg ttttgggtgg agaaataggc tgctttgggt agccaagtag
     2761 tcgaacaggt tggtgaccgg tttgggcaag cacgagggat ggctcaggaa ggcgtcccgc
     2821 aggggttgat agctgtcttc gccattttcc gcgccatgtt aaggtcaaca ctgggtgaac
     2881 tccaggtggt cagaaattga cgctctttcg aagcgcgagg taggatggaa tatgactcct
     2941 gaagtaggtt gggtcggaat cgaggaagtg ggcgcccatc caaaggtggc taagcagtcc
     3001 atctaccgct gggtggtttc aaagggcttc ccggcccacc gtgtgggacg cctccttcgg
     3061 ttcagactct cggaggtgga cgagtgggta acgtccagca gcggcggtga tttcgcgtcc
     3121 gccccgtcgg gcaagacgac ggaatcgagg ccagcaggcc gaaagacgaa cgtgaagaag
     3181 aagagcaggc atggctgaga gccgcttttc aacgcagatc ggacggcaga tctccttgcc
     3241 ggggcacttc gatgttcctg tcatcctcga ggacgtacgg gcgctcggtg gggacggctc
     3301 cggcggctac gagtgccggg tccggttgcc cgacggcacc ctcgaggagg cagtcatctc
     3361 ccctgcggaa gcggccacgc tcttcagcac ggaccgggaa tcgccgacag cgatcacgcc
     3421 agctgatgcg gaacaactcc gactgttgat cgagtccgcc cggattcgcc tcgcctatgc
     3481 ccacgaccgt cagtttgccg tgagcctttc cggcatccgc acgctgcctc accagatcga
     3541 ggccgtgtac caggccatgc tgccgcagcc tcgcctgcgc tttctcctgg ccgacgaccc
     3601 cggggcgggc aagactatca tggccgggtt gctcatcaag gaactcaaac ttcgggaggc
     3661 catcgagagg attctgatcc tctgccctgc gccgctcacg atccagtggc aagacgaaat
     3721 gctccgctgg ttcggggagt ccttcgacat catcttctcc gccgttgacc agcagcagct
     3781 cacgaacccg tggcagcggt gctcgcaggt tatctcttcc atcgactacg cgaagcagaa
     3841 cgacgtccgc gagcgcgtgt ggcaacagcg ctgggacctg gtcgtcattg atgaggccca
     3901 taaatgctcg gcttacacga agtcgcgtac caaccggagc gacgaggttg ccgagaccaa
     3961 gcgctatcaa ctggccaaac gcctctccga gacggcggac catgtgcttt tgctgacggc
     4021 aacgccccac cacggggacg aggacaagtt ttcgcacttc ctgcggctca ttgatcccga
     4081 cctgttcccg gagccgcatc ggctcgggaa tcaagccacg tcgattcgca aggacgtgtt
     4141 caagctcggc aaggactcgc cgtggtgcct gcgccgcctg aaagaggatc tcagggatct
     4201 caacggcaag cgcttgtttc ccgaccggca cgccaggacc gtgaccttcc gcctcaacag
     4261 cgaggagtac gctctctaca agtcggtcac ggcgtacatc aacgagttca ttccgggagc
     4321 ccaagcgtcc tcggctggag gacaacgacg gtcggctgcc gcgctcaccc gcaccgtcct
     4381 ccagcgacgc ctggtgagtt cgacctgcgc catccatgag tcgctgaagc gacgcctcaa
     4441 gaagcagcag gacctgcttg acgagttgga ggggctctcg ccggcccagc gggccaagcg
     4501 tctcgccgcc cttcagaaac ggctacccga tgcagagcaa gaagaggatg acctcgacga
     4561 cgaggtccgc gatcggctgg tcgacgagta cacagcagcc ctcgagctgg agcaactccg
     4621 cgcggagatc tccgccctca aggagctggt cgagcaggcc cgcagggtac aggatctaga
     4681 gagggattcc aagttggccg cgctgaaaaa gtgcctccaa gaggcgcagt ttgccgaact
     4741 cagggacggc cgcggcaaat tactgatctt tacggagcac cgcgacaccc tcacctacgt
     4801 gcgggaacac ctcgagcgct ggggattcac tacctgcgag atccatggcg gcatgaaccc
     4861 ccacgagcgc aagcgggcac aggaacagtt ccgcaccacc gcgcaggtct gtgtggtgac
     4921 cgaagcagct ggcgaaggaa tcaacctcca gttctgccac ctcatgatca attacgacat
     4981 gccgtggaac ccgacgcgcc tggagcagcg ccttggccgc atccaccgca tcgggcagga
     5041 cagggatgtc tacgccttca actttgtggc cacggagtcc gaggacgggc agcccatcgt
     5101 cgagggacgc atcctccatc gactccttga gaagctggac acgatgaaag aggccctcaa
     5161 aggacgtgtg ttcgacgtta tcggcgaggt gctttcgctt aacgacgtca accttcccga
     5221 catgctgcga gaag
//
LOCUS       DAOPTR010000116         5198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_12927_length_5198_cov_2.945363, whole genome shotgun sequence.
ACCESSION   DAOPTR010000116 DAOPTR010000000
VERSION     DAOPTR010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12927_length_5198_cov_2.945363"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PKI05_03450"
     CDS             <1..412
                     /locus_tag="PKI05_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51284.1"
                     /translation="SLTGASLALIVTEPTVAGLHDFERIARLCRQLGVPAVTVINKAD
                     INPETSDRIANSSIRYGTDVIGMIPFDSDIVRAQMAARSVVEVSDGPAARAMRAIHST
                     VMSRLEKALDVHSGEFITIDVTQIDNVKSKGVTT"
     gene            409..747
                     /locus_tag="PKI05_03455"
     CDS             409..747
                     /locus_tag="PKI05_03455"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOA51285.1"
                     /translation="MRIAIPLSGKTLSSHFGHCEQFAFVEVDDQARQVKGVTFVQPPP
                     HQPGLLPRWLHEQGVTTVIAGGMGPRAQQLLSELGIDVILGATGLHPEKLAADYLAGQ
                     LAIGENRCSH"
     gene            complement(780..2471)
                     /locus_tag="PKI05_03460"
     CDS             complement(780..2471)
                     /locus_tag="PKI05_03460"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008669328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOA51286.1"
                     /translation="MVFLRCGSAVCAWGVKLFGVLALGLGAGPMQSILGGERPNIVII
                     LVDDMGWSDIGCYGSEIPTPHLDSLAKNGLRFTQFYNTGRCSPTRASLLTGHYPHQAG
                     MGHLDGTFVPNHPGYQARIADSSVTMAEVLKEAGYFTAMTGKWHLGQNRGTPPWSRGF
                     MRSLNLAAGGVHFPGQTWRKEAKLYLNGEEKAPDDPLFGDNWYGADLWTEWGLKFIDE
                     ALDAKKPFFLYLAHCAPHFPLMAPSADIAKFRGQYLVGWDKLREDRYRRQIEMGLIDP
                     RWPLSPLPENTPPWESVSEAERDRFDQIMAIYAAMIYAMDRSVGTLVAGLKQRDVLDN
                     TVIFFLSDNGGNAESGPNGRCEGDNPGDRHSVVFLGQNWATLNNTPFRKWKHYVHEGG
                     SATPLIVHWPAGLDAGQRGQLYHQPAHVIDLMPTVIQLAGATYPTQYNGHTIEPMEGV
                     SLVPAFSGKPIVRSQPLFFNHEQNRAVRDGKWKLVALAGQPWELYDMEADRTELHDLS
                     AQASERVAAMAAQYEAWARRTHVIVEGIPDPFDKPARKVQAQKRQQPSQVAPRTP"
     gene            complement(2683..3714)
                     /locus_tag="PKI05_03465"
     CDS             complement(2683..3714)
                     /locus_tag="PKI05_03465"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOA51287.1"
                     /translation="MDQVLQDSGGLLSRPVSNLQPQFVLDAPSPLAVLQASGVTIAVY
                     DNADEMGLASAIHLAAEQCRLVETQGKASFIIMAAPSAFAFYRAYVRLVECSHRLQEA
                     CRQTHFFQFDEYPLPAHHPASFRYLLMKHFFTPLGTFYDPAKVHFFEADALDPEAAAC
                     QYEQALLEHGLDLQIMGIGENGHWGFHEPGIPLEDRPRFMRVALTSENVDQQMRDHPS
                     IFKSVEDVPRVAYTANVAMFLTTRHVIEGNVSQASKAFALLAAFGSNIVHECVPASAL
                     KRHGRGIVRTTRAAAWALMDYQQRGLVSREALLRLAESLRGPASSDIQAIVSRICQVF
                     DTMQIQYER"
     gene            complement(4167..>5198)
                     /locus_tag="PKI05_03470"
     CDS             complement(4167..>5198)
                     /locus_tag="PKI05_03470"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOA51288.1"
                     /translation="GFRRLIAMAFPEPEYALQMLPPEHPVWRAEEPIPSQYLRPLLGV
                     EFGCRTSVIYAPPTAVSPSQPRPSLSCLWELSRAGRGVTFAPAVQAEITAGLNLGLNV
                     LAYATNRQLKYKYAFFNQQSSRAVRDNVRRNKIAVAELLHPGGCHVAPRALNNLLEAA
                     ADQLRLRVELSKNEIAISDPALFDFHLAFMHGRNAFRLTDEERKQLRLYLERGGMILA
                     DAVCANRAFAESFRREFGNLFPDHPLQPIPPDDPLFTEQYGGFDLRFVTRRDPQPTDN
                     TGKSEEMFRQVRPELEGIRLDDRWVVIFSPYDLSCALERQQAIGCRGYSPEDAARIGI
                     NVLLYSLQQ"
BASE COUNT         1198 a         1449 c         1460 g         1091 t
ORIGIN      
        1 gtctttaacg ggcgcgagtt tggccctgat tgtgaccgag ccaaccgtag ccgggctgca
       61 cgatttcgaa cgcattgcgc ggctttgccg gcaacttggt gtgcccgccg tgactgtcat
      121 caacaaagcg gatattaatc ctgaaacgtc tgatcgcatc gccaacagca gcataagata
      181 cggaacagat gtaatcggta tgattccttt tgattccgat attgtgcgtg cccaaatggc
      241 ggcgcggagt gttgtggaag tctccgacgg tcccgccgct agggcgatga gggctattca
      301 ttccaccgtc atgagtcgcc ttgagaaggc tcttgatgtc cacagcggtg aattcataac
      361 catcgatgta acgcagatag acaatgtgaa gtcgaaagga gttacaacgt gagaattgct
      421 attccccttt ccggcaagac gctgtcttcc cattttggac attgtgaaca gtttgccttc
      481 gtcgaagttg acgatcaggc tcggcaggta aagggtgtga ctttcgttca gccgccgcct
      541 catcagcctg gtcttctgcc ccgatggctc cacgagcagg gagtgacaac cgtcattgcg
      601 ggcggcatgg ggcctcgtgc ccagcaactc ttgagtgagc tgggaatcga tgtgattttg
      661 ggagcaacgg gattacaccc ggaaaagctt gccgccgatt acctggcagg tcagcttgca
      721 atcggtgaaa accgttgtag tcactgatta cctgcgccgt tgacaaaaag aggcagatct
      781 taaggcgttc tcggggccac ctgcgacggc tgttgccgtt tttgggcctg gacctttcgg
      841 gcgggtttgt caaaggggtc agggatcccc tccacaatca catgagttcg cctggcccag
      901 gcttcatact gagcggccat ggcggccacc cgttcagagg cttgtgccga caaatcgtgc
      961 aattccgtgc gatcggcctc catgtcgtac agttcccatg gttgacctgc cagggccacg
     1021 agtttccatt ttccgtcgcg caccgcgcga ttttgctcgt ggttgaaaaa gaggggctgc
     1081 gaccgcacga tgggcttccc agaaaaggct ggcacaaggc taacgccttc catgggttcg
     1141 atggtgtggc cgttgtattg cgtggggtaa gtagccccgg caagctgaat caccgtgggc
     1201 atcaaatcaa tcacgtgggc aggctggtgg tatagttgcc ctcgctgacc ggcatccagg
     1261 cctgccggcc aatggacgat caacggcgtg gcactgccgc cctcatggac atagtgcttc
     1321 cacttgcgga aaggcgtatt gttgagcgtg gcccagttct gccccaaaaa cacaacagag
     1381 tggcggtctc ctggattgtc cccttcgcaa cgaccgtttg gcccggactc tgcgtttcca
     1441 ccgttatcac tgaggaagaa gatcaccgtg ttgtcgagga cgtcgcgctg ttttaaaccc
     1501 gccaccaacg tgccaacact ccggtccatg gcataaatca tggcggcgta aatggccatg
     1561 atttggtcga accgatcccg ctcggcttcg gaaacgcttt cccacggcgg tgtattctcg
     1621 ggaaggggcg acaaaggcca ccgcggatcg atcagcccca tttcaatttg tcgccgatat
     1681 cgatcttcac gcagtttatc ccagcccaca agatactgcc cacgaaactt ggcgatgtcg
     1741 gctgatgggg ccatcaatgg gaaatggggg gcacaatgtg ctaaataaag gaaaaacggc
     1801 tttttggcat ccagggcctc gtcgatgaat ttgagtcccc attccgtcca caggtcggcg
     1861 ccgtaccagt tgtctccaaa aaggggatcg tccggggcct tttcttcacc attcaaatag
     1921 agcttggctt ccttccgcca ggtttgccca gggaaatgga cacctccggc ggccaggtta
     1981 agactccgca taaagccccg gctccacggt ggcgtacccc gattttgccc aaggtgccat
     2041 ttgcccgtca tggctgtgaa gtatcccgct tccttcaaga cctcggccat cgtgacactg
     2101 gaatcagcga tgcgcgcttg atagccggga tgattgggga cgaacgtgcc atcgagatga
     2161 cccatcccag cctgatgggg ataatggccc gtgagaagtg atgcccgtgt cggcgagcaa
     2221 cgccccgtat tgtaaaactg cgtgaaacgt aggccattct tggccaggga gtcgagatgc
     2281 ggcgtgggaa tttccgaccc ataacaacct atgtcggacc accccatatc atccacaaga
     2341 atgatgacga tattggggcg ctcaccgccc aaaatagact gcatcggccc cgcgccaagt
     2401 cctaacgcca ggacgccgaa gagcttgact ccccaagcgc atactgccga cccacatcgc
     2461 aaaaagacca tgttatgcct cccactaaaa agccttttca tcgtacgaaa cgatggccct
     2521 attataagag ctgtttgatc agacattcaa ggaataaata ggacgatcgc ggcacctcca
     2581 actgacgtta agcaaatcca tggtgcgagg gcagacacac cgaccgaatg agctgatccc
     2641 gggccaaagc cacgaagacg caagcccatg tttcgggcaa gctcatcgct catattggat
     2701 ttgcatggta tcgaagacct ggcaaatccg agaaacgatg gcttggatgt cagaagatgc
     2761 cggtccacgc aggctttccg ccaaacgcaa gagggcttcg cgggaaacta gaccacgttg
     2821 ctggtagtcc atcaaagccc aggcggctgc ccgcgttgtg cggacaattc ctcgaccatg
     2881 ccgtttcaag gcactggcag ggacgcattc gtggacgata tttgatccaa aggccgccaa
     2941 cagcgcgaat gccttgctcg cttgcgacac attgccttcg atgacatgac gtgtcgtcaa
     3001 aaacatggca acattggcag tatatgccac tcgaggcaca tcctcgactg atttgaagat
     3061 cgatgggtga tcgcgcattt gctgatcgac attctcgctc gttagtgcga cgcgcataaa
     3121 gcgaggccta tcttcgagcg ggatgcctgg ctcatgaaac ccccagtgtc cattctcacc
     3181 aattcccatg atttgcaagt cgaggccgtg ctccagcaac gcctgctcgt attggcacgc
     3241 ggcggcctca ggatccaagg cgtccgcctc gaagaaatgc actttggccg gatcatagaa
     3301 tgtcccaagc ggagtgaaaa agtgtttcat tagcaaatag cgaaaagatg cggggtggtg
     3361 agccggcaat gggtactcgt caaactgaaa gaagtgcgtc tggcgacagg cctcttgcaa
     3421 gcgatgcgag cattccacca gtcgaacata tgcgcgatag aaagcaaaag cagatggggc
     3481 cgccataatg atgaagctgg cttttccctg ggtctcgact agacgacatt gctcggcggc
     3541 caagtgaatc gccgaggcca aacccatctc atctgcattg tcgtaaaccg cgattgtgac
     3601 accgctcgct tgaagaacgg ccaagggtga cggggcgtca agcacaaact gcggttgaag
     3661 attgcttacc ggtcgcgaga gaaggccgcc agaatcctgc aaaacctggt ccatgatgat
     3721 ccccttctga ttcttcgaaa gacaccaaaa gcgaaaacac acggcctccc catcaaccgg
     3781 taaaaccggc cgatcaaatc atattcgtta accggtcgag tataaagacg ccgccgaccc
     3841 tgtcaataag cctggtggaa ccgtctcttc cgaatggttc ctcggcgcaa ccgtagcgcg
     3901 tgagaggcgg agacagccgg tgcagggagc gccacgctcc gtcatggccg tatcaatcta
     3961 aacgcacgca cggcgaaaat ggcagatgcg ggggaccgcg tctctttcgc cttagcggac
     4021 gcgacagtct tatttgttaa gtaagagaga gtgcccctgc tcccctcaca aaacgtgccg
     4081 gcatcagacg gcggtcgctg ttatcaatga gtgttccgaa tccaagattg ccacgccaac
     4141 acgtttttgg caaacggcca tcgggcctat tgctggagtg aatagaggag gacgtttatt
     4201 ccgattctgg ccgcatcctc ggggctatag ccgcgacacc cgatggcctg ttgcctctcc
     4261 agtgcacagc tcaagtcgta tggagaaaag ataacgaccc aacggtcatc caagcggatg
     4321 ccttccagtt cgggccgaac ctggcgaaac atctcttcgc tcttgccagt attgtcggtg
     4381 ggctggggat ctcgccgggt aacgaatcgt aggtcaaatc ccccatactg ttcggtgaag
     4441 agaggatcat caggcggaat aggttggagg ggatggtcag ggaagagatt cccgaactcg
     4501 cggcggaacg attccgcaaa cgcacgattg gcacacacgg cgtcggccag gatcatacca
     4561 cccctttcca gataaagccg taattgtttt cgctcttcgt cggtcaaacg aaaagcattt
     4621 ctcccatgca taaaggccaa gtggaaatcg aagagggccg gatcggagat ggcgatctca
     4681 ttcttcgaca gctccacacg gaggcggagt tggtcggctg ccgcttccaa aagattattc
     4741 aacgcccgcg gtgccacatg gcaacctccc gggtggagca attcggccac ggcgattttg
     4801 ttgcggcgaa cattatcccg taccgcgcgg gacgactgct ggttgaaaaa ggcgtatttg
     4861 tatttgagtt gccgattggt tgcgtaggcc aggacgttaa gacccagatt cagccccgcg
     4921 gtgatttctg cctgaacggc cggcgcaaag gtaacgcctc ggccagcccg cgacaactcc
     4981 caaaggcagg atagcgaagg cctgggctgg ctgggggaca ctgctgtggg cggcgcatag
     5041 atcacactgg tccggcagcc gaattccacg cccaacaatg gccgaaggta ttgcgagggg
     5101 atcggttctt cggcccgcca caccggatgc tcaggcggta gcatctgcaa ggcatattcg
     5161 ggctcgggga acgccatcgc tatgagtcgg cgaaatcc
//
LOCUS       DAOPTR010000117         5176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_13003_length_5176_cov_3.757665, whole genome shotgun sequence.
ACCESSION   DAOPTR010000117 DAOPTR010000000
VERSION     DAOPTR010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13003_length_5176_cov_3.757665"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="PKI05_03475"
     CDS             <1..1311
                     /locus_tag="PKI05_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOA51289.1"
                     /translation="GWASEVNRPNFLVIIADDMACEDCGAYGHPHIKTPNIDQLAREG
                     MLFERAFLTCSSCSPSRTSLLTGRYPHATGAAELHQPVPPDQLLVTTPLREAGYFTAA
                     AGKWHLGPHVKSQFDVVREGGGDGAYDYWLPVLKERPKDKPFFIWLATTDPHRPYGPN
                     RIPEPHTPQDVIVPPYLPDVPEVREDLALYYDEIGRLDTWVGRIREELIAQGVWDQTM
                     VVFLSDNGRPFPRCKTTVLDSGVRTPLIVRWPGRIPAGSVSSRLVSAVDLAPTILELA
                     GITPGPSFQGRSFAKLFDDPTAKIRDYVYAEHNWHDYQAYERSIRNERFLLIKNWLPQ
                     LPRTPPADAVRSPTYDAMKRLRAQGKLTPPQMEPFVVPRPEVELYDCENDPHNLTNLA
                     DRPEYSTIKSELLRALETWREQTGDSVPEPLTPDKFDRETGKRL"
     gene            1979..2245
                     /locus_tag="PKI05_03480"
     CDS             1979..2245
                     /locus_tag="PKI05_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51290.1"
                     /translation="MNFFTWIREGVRQAVLLGVADAVDSLGTSPDGGDYRQRIGEYLR
                     ASPDAGQWKRLAISSPAASGGRKKLGRGLEDIQAVVNPPNDARK"
     gene            complement(2381..3622)
                     /gene="fabF"
                     /locus_tag="PKI05_03485"
     CDS             complement(2381..3622)
                     /gene="fabF"
                     /locus_tag="PKI05_03485"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOA51291.1"
                     /translation="MKRRVVITGMGVVTPLSCQVEDLWERLCRGQSGIRLIEGFDTSI
                     FRVKFGGEIRNWNPSGYIDAKEAKRLDRFAQYAIVAATDAIRQAGLDFSREDPYRCAA
                     IIGSGVGGLGEIEMQHRRLLEKGPERVSAFTIPKMMLNAASGCVSIQFGIKGPSYGVV
                     TACASASSAVVEAAKGIQFGEIDVAITGGTEAAVTPLGIAGFTAMSALSERNDAPEKA
                     SRPFDRDRDGFVLSEGAGIVVLEELEHAKRRGATILGELLGYGCSSDAGHITQPDENG
                     TGAARAMADALRNAGLNPEDIGYINAHGTSTPLGDRAETVAIKNVFGDYAKKVSVSST
                     KSQLGHLLGASGGVELIISLLAVRKGVIPPTINYETPDPECDLDYTPNVPRERRMKAA
                     MSNSFGFGGHNVSLIVGEFRD"
     gene            complement(3619..3891)
                     /gene="acpP"
                     /locus_tag="PKI05_03490"
     CDS             complement(3619..3891)
                     /gene="acpP"
                     /locus_tag="PKI05_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOA51292.1"
                     /translation="MASIEERVINIVAESLATEKDKITRDSHFVNDLGADSLDLAELI
                     MALEDEFDISIPEEVTSEIQTVGQAIDHIKAAVEGEGSTATSDEHA"
     gene            complement(4163..4933)
                     /gene="fabG"
                     /locus_tag="PKI05_03495"
     CDS             complement(4163..4933)
                     /gene="fabG"
                     /locus_tag="PKI05_03495"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOA51293.1"
                     /translation="MEKDSPRIQVNLHGKVACVTGAGRGIGRAIALTLGRAGAAVACV
                     DVNGQAAESVAEEIRALGKAQAVGFACDVTNSEQVTQTVEAIVEKLGGLHILVNNAGI
                     TRDGLLLRMKDDQWDSVINVNLRGTFLFTRSAARYLIRAGGARIINIASISGLMGNPG
                     QANYSASKAGVIGFTRTVAKELASRQVTVNAVAPGFIATEMAQALGEQVLEEIKKRTP
                     LGRLGDPQDVADVVLFLASEAASFITGTVIRVDGGLAE"
BASE COUNT         1120 a         1579 c         1335 g         1142 t
ORIGIN      
        1 ggatgggcaa gcgaggtaaa tcgccccaat tttctggtca tcatcgccga cgacatggcg
       61 tgcgaggact gtggggccta tggccatccc cacatcaaaa cccccaatat tgaccaactg
      121 gcacgtgaag ggatgctttt tgagcgggcc tttctgacgt gcagttcatg cagtcccagc
      181 cggaccagtc tccttacagg tcgctatccc catgccacag gggcggcgga actccatcaa
      241 ccggtaccgc cggaccagct tctcgtcacc actccactcc gtgaggcggg ctatttcacg
      301 gcggcagccg gaaagtggca tctcggtccg catgtgaagt cacagtttga tgttgtccgg
      361 gaaggcggag gcgacggggc atacgactat tggctgcccg ttcttaagga acggcccaag
      421 gataagcctt tcttcatctg gttggccacc actgatcccc atcggccata cggccccaat
      481 cgtattccgg agccacacac tccgcaggat gtgatcgttc cgccatactt gccggatgtt
      541 ccagaagtgc gggaagacct ggccctttac tacgacgaaa ttggtcgatt ggatacgtgg
      601 gtcggtcgga ttcgggaaga gctgatcgct cagggggttt gggatcaaac gatggttgtc
      661 tttctttccg ataatggcag gcctttccca cgttgcaaaa cgacggtcct tgatagcggt
      721 gtacgaactc ccttgatcgt ccgctggccg ggaaggattc ctgccggtag tgtgtcatcg
      781 cgtttagtta gcgcggtgga cttggcacca acgatcctgg agttggccgg tattacgcct
      841 ggtccttcat ttcagggacg gagctttgcc aagctgtttg acgatcccac cgcaaaaatt
      901 cgggactacg tttacgccga gcataactgg cacgattatc aggcgtacga acggagtatc
      961 cgcaacgagc gattcctcct catcaagaac tggctaccgc aacttccacg gacaccaccc
     1021 gccgatgccg tacgaagtcc cacctatgat gccatgaaac ggctgcgagc tcaggggaaa
     1081 cttacaccgc cgcaaatgga gcccttcgtg gtaccgcggc cggaggtgga gctgtatgat
     1141 tgtgaaaatg atccgcacaa tctgaccaac ctcgcggacc gaccggaata ctcgaccata
     1201 aagtccgaac tcctgagggc cttggaaacc tggcgagaac agacgggtga ctctgttccc
     1261 gaaccattga cgcccgacaa gtttgatcgt gagacgggaa aacggttgta aatctgggat
     1321 aaaagggccg tcattaatgc ctatcccata atctcccctc gcccgttcgg cgggagacgg
     1381 gtcgggggtg agggtggctt gaatcactgg cggttttctc aatcgattcc gacgatcgct
     1441 tgccctcacc ccaaccctct cccggcggga gagggagact ttttgggatg gactctaaat
     1501 ccacgggata acagataagc caggcaacgc agataatcga ggcatttcga ccgggtagtg
     1561 cgcactggcg gttggtgata gccgtttcgc gcccgaacaa aaaacagaca tgacggcgtc
     1621 ccttcctccg gattgagcag tcagtcctca tctcaggatg gggggaccaa ttgtacgttc
     1681 tccaaccgtg atacgggcaa aacttatggc cgatccaccg tgtgggagat cacgcgcccc
     1741 ggtttggcct ttttgcccag tggagggcca cactttgtcg taaccgtacc aaactgtgcg
     1801 tcttccaatt tcccgcgggg gaaaacctac ggattacttc ttttttccgc agttacccac
     1861 tcggccacct gaaggaaatc gctctggttc ggcgcgacgg attttgctaa tctctcatct
     1921 ccttgggtcc gccgtacagg agcagattcc tttagaaaga aaattgcagg aggaacacat
     1981 gaacttcttc acatggattc gcgaaggtgt ccgtcaagcc gtactattgg gtgttgctga
     2041 cgccgtcgat agtctgggga cgtctccgga tggaggtgac tatcggcagc ggatcggcga
     2101 atatctgcgg gcaagcccag atgccggaca atggaaacga ttagccattt catcgcccgc
     2161 tgccagcggc ggccgaaaga agttgggacg gggactcgaa gatattcagg ccgttgtgaa
     2221 tcctcccaat gatgcccgca agtaactccc acggattgcc tttcgcaatt cgttagtatc
     2281 gcagcggcga ccaggggcga aaaatgaaca aacccggctc ggacgtatct tccggaccgg
     2341 gtttattttt tgacgtcttc gcttacgtcg gcgaaagagc ctaatcccgg aactcgccga
     2401 cgatgaggga aacgttgtgt cccccaaaac cgaagctatt ggacatggca gccttcatgc
     2461 ggcgctctcg cggcacattc ggcgtatagt cgaggtcaca ctctggatcg ggcgtctcgt
     2521 aattgatagt cggcggaatc acacccttcc tgacggcaag gagagagatg atcaactcta
     2581 cgccgccact cgcccccaac agatgtccga gctgactctt ggtgctggac acgctcacct
     2641 tcttggcgta atcgccgaac acgttcttga ttgcgacggt ctcagcccga tcgccaagtg
     2701 gggtactggt cccatgagca ttgatatagc cgatatcctc agggttcaag ccggcgttac
     2761 gcagggcatc cgccatagcg cgggcagctc ccgtcccgtt ttcatccggt tgggtgatgt
     2821 ggccggcgtc cgaactgcac ccgtagccaa gcaactcacc aaggatcgtc gccccgcgcc
     2881 gcttggcgtg ctccagttcc tccaagacca caatcccggc accttcgctg agaacgaatc
     2941 cgtcgcggtc gcgatcgaac gggcgactgg ccttttccgg ggcgtcattg cgctcggaaa
     3001 gggcgctcat tgcagtaaaa ccggcgatgc ccaatggggt aacagcagct tccgtaccgc
     3061 ctgtaatggc cacgtcaatc tcgccaaatt ggattccctt ggccgcttcc acgactgcgc
     3121 tcgaagcact ggcgcacgcc gtgacgaccc cgtaactcgg tcccttgatc ccaaattgga
     3181 tcgagacaca gccactcgcg gcattcaaca tcattttggg aatcgtgaat gccgataccc
     3241 gctccgggcc tttctcgaga aggcggcggt gctgcatttc gatctcgccc aggccgccga
     3301 cgccggaacc gatgatggcc gcgcagcgat agggatcctc gcggctgaaa tccaacccgg
     3361 cctgccggat tgcgtccgtt gccgccacga tcgcgtattg ggcaaaccgg tcgagccgtt
     3421 tcgcctcttt ggcatcgata taaccgctgg gattccaatt ccggatttcc cctccaaatt
     3481 tcacgcgaaa gatggaggtg tcaaatcctt caatcagacg gatcccgctt tgcccccgac
     3541 agagtcgctc ccagaggtct tccacctggc aactcagcgg tgtgacgaca cccattccgg
     3601 taatgacgac acgtcgcttc atgcgtgttc atccgaggta gctgtgctgc cttcaccttc
     3661 cactgcggcc ttgatgtggt caatggcctg tccgacagtt tgaatttcgg acgtcacctc
     3721 ttccggaatg gaaatgtcga actcgtcctc aagcgccatg atcaactcgg ccaaatcgag
     3781 cgagtcggca cccaagtcgt tcacaaagtg agaatcgcgg gtgattttat ccttttccgt
     3841 tgccaaactc tctgcaacga tgttgatgac acgttcttca atcgatgcca cgacagtcat
     3901 cctccttttc ttctcaattg ccaaccgccg atggggacaa gcacgcccaa acggccaata
     3961 ctccactgac tcacttccgg attgaagcga ttcggaaacg acgtctcccc caaaacaaaa
     4021 tacgcccctt tttgcctcaa ccgcaattaa attacgttac agggttttct cttaagtgtc
     4081 cataccgaga agcccctcgt gaagcggcac cacatccttt ccccactggc cgccaaccca
     4141 cgcggcgctg gcctgttccc tattactcgg ccagaccgcc atcaactcgg atgaccgtcc
     4201 ctgtgataaa agaagcggcc tcacttgcca ggaataaaac gacatcggcc acatcttgcg
     4261 gatcacccag ccgccccaaa ggtgtgcgtt tcttgatttc ttccagcacc tgctctccca
     4321 aagcctgggc catttctgta gcaataaaac ccggcgccac ggcattcacc gtgacctgcc
     4381 gcgaggccag ctccttggct accgttcggg taaacccgat gactcccgcc ttggatgccg
     4441 aatagtttgc ttggcccgga ttacccatca accccgaaat actggcaatg ttgataatcc
     4501 gggctccacc ggcacggatc aaataccggg ctgcactcct agtaaacagg aacgttcctc
     4561 gtaagttcac attgatgaca ctgtcccatt gatcatcctt catccgcaac aagaggccgt
     4621 cccgcgtgat cccggcgtta ttcactagga tatgtaagcc acccagtttc tcaacaatgg
     4681 cctcgacggt ttgggtaact tgttcactgt ttgtaacgtc gcatgcgaac ccaactgctt
     4741 gggcctttcc cagcgcgcgg atttcttcgg ccaccgattc cgccgcttgg ccgttcacat
     4801 caacacatgc cacggcggcc cctgcacgtc ccaacgtcag ggcaatcgcc cggccaatgc
     4861 cccgtcccgc ccccgtaacg caggccacct tcccatgtaa attgacttgg atccgcggag
     4921 aatctttttc catgacctat tctcggcctc acactgatac tcgcttctgg tgtccccttg
     4981 ccgaaaaaat attgacttgc gctatcccta tttggacatt tatacctaac tcttgtggca
     5041 acagccttgg cgaatactcg atcacacctc gatattctgg cacgtgacac tccgttcaat
     5101 ccgacggagc agcccgcgca gcacccgccc cggccctact tcataaaagc tatcgaatcc
     5161 ctcgccgatc aaataa
//
LOCUS       DAOPTR010000118         5173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_13021_length_5173_cov_3.242087, whole genome shotgun sequence.
ACCESSION   DAOPTR010000118 DAOPTR010000000
VERSION     DAOPTR010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13021_length_5173_cov_3.242087"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PKI05_03500"
     CDS             complement(<1..326)
                     /locus_tag="PKI05_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOA51294.1"
                     /translation="MSRRVSLGILLMCLGLIGLCDYASAASPSLWPQWRGPQRDGILS
                     DFTAPEKWPETLKQVWQVTVGSGDSTPALVDGRLYLHTRQGDEEVLLCLDAYSGKEIW
                     TNKYPSP"
     gene            596..1066
                     /locus_tag="PKI05_03505"
     CDS             596..1066
                     /locus_tag="PKI05_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51295.1"
                     /translation="MPDLESVIFSTAVLAIAAAMMWRPAVRFWRRVRFEKARMLFHRE
                     RERLEARLIKQIAVPVVTGEMEWLDCDFEDEVTFLRDRRSGEYVAVVMITLGPETSWA
                     IDSDQESRGRGVAVFRFGKNRWISDPKVYMNLTPEEMVRAFSDRMELVRREMTR"
     gene            complement(1125..2468)
                     /locus_tag="PKI05_03510"
     CDS             complement(1125..2468)
                     /locus_tag="PKI05_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51296.1"
                     /translation="MFSPYRVKVFIHFEPGLLSAEDDVHQFHKQVAAVLRPMLGNRWD
                     FEVTGFPTEVISRSLDLRTPPPGDRLPPSWKKITDADKITVVQTIRTGVGWTVQAREW
                     DVRLERWGPLRIVSTSTGSRLCDVVAQGIVSAHVPIGEIQVVDERTVHVAIRGGLLSE
                     ADEGVPSLDVPDLFVAVARYEDREGNARAVIPLPWTVLKRSSDVGAEQPTGITCEVVS
                     GIRNALSARRRGRVKLYAQAVQPSGAQPVTLRLQSRDPPRGPLVGYEIYTQPLGEGSP
                     IFVGRTDAAGQAALPAGDASWQLISIKHGRRLLARVPLVPSEGSLAELTLPNDDPRLV
                     AETYLTGLQDELVDLVTLRTILIARMRARMIAEDWEAAQRIRDNLLKLKPREIFSSEL
                     TRQQQRIFCPDPVGQKQIDAMFSEMQRLVNIYLNPQEVESLVKQLASRQKPQPGS"
     gene            complement(2756..>5173)
                     /locus_tag="PKI05_03515"
     CDS             complement(2756..>5173)
                     /locus_tag="PKI05_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA51297.1"
                     /translation="LLLTTFHQLHRKRHAVQAVILAIISFLVCIYLYAEQAKDSSSAG
                     ARTPAEQIAETIKSLIDEKVPLYDQAPFDLIILDAANNHAVLKVAPLALPDRKLPQPL
                     PKTGYLDLELFDEPGERYRLPWRNIVEIRLFERLLIEKATQLIREKRFGEAYDYLDHL
                     SQLYPKAEGLSEVQQLFLHEEAVDALTSKTFDRAYANLLNLYRLNPQYPGLADHLSQA
                     AEGLILRYLEASDYASARGVVDSLAQLTPQHPLVQRWQKVWQQQAEDLRTQIEAALAS
                     GQPRQADRLLRQLRQIWPALTGLPELSNRVQEAYPRLRVAVTLPAISFDPNSLTDWAA
                     RRSGRLLYRSLLEYVAPGGEGGVYQSQIIDWKIEELGRRLVFQLRPDRPPFHASGSVY
                     TTYDLAHQLLAMVDPVDQNFTSEWSVLLNRVRMVSPDVVIVELRHAHVRPEGLLRWIP
                     TPGWNLTGTGEALTPENGPFTRSKEESDDKTAVYLRTHSGQQAPGLLEIEEIAFSRGR
                     DAIRALKQGNVDMIDRVNPWELHLLENDASISIASYRVPLVHMLVPNFSRPLPANRDF
                     RRAMLYAIDRPRILKAILNQRNLPGCQVVSGPLPVGRRFDDPLGHGYDTQIAPRPYQP
                     SLALALADLAYRQTVEIAKNRGLPVPARKKLILVHPPHEIAREACSQIKRQWQLIGWE
                     TTLVELHPGSPDLPSDFDFLYVEAAVWDPITDLARCFALIRRSQFFPTQVDAALSQLG
                     QAVDWPDASSRLKTLHRRIYEDTTVLPLWQLTDYYAFRKGIQGIETERATLYQNVELW
                     TLSDSGR"
BASE COUNT         1190 a         1350 c         1528 g         1105 t
ORIGIN      
        1 gggctggggt atttgtttgt ccagatttct ttcccggaat aggcgtccaa acacagtaag
       61 acctcttcat ccccctgccg tgtgtggagg taaagacgtc catccacgag ggctggtgtt
      121 gaatcgcccg aacccacggt gacctgccag acctgcttta gagtttccgg ccatttttct
      181 ggtgccgtaa agtccgagag aatgccatcc cgttgcggac cgcgccactg gggccacagg
      241 ctcggggatg ccgcactggc ataatcacaa agcccgatca agccgaggca catgaggaga
      301 ataccgagag aaactctgcg tgacatggcg gacctccttc tgaagttgag ccgtcgagaa
      361 gacagaaccc acctgttgcc gctggttggg acgttacttc attggcaaag gctttctgtt
      421 tgagcattag tttatagcca gagacagtgt caatcaacga gagcgcccag acccacaaac
      481 aatgggcacc ggactccgaa agaacggaaa gtcccactgg ttgcatttcc tcggagtccc
      541 tctctagaat gctagtaggc gaggtggtcc tcattccggg gttgggggcg ttgagatgcc
      601 ggacctcgaa tcggtcattt tctcgacggc ggtattggcc atcgccgctg caatgatgtg
      661 gcggccggcg gtacggttct ggcgtcgtgt ccgattcgaa aaggcgcgga tgctttttca
      721 tcgtgaacgc gagcgcttgg aggctcgtct catcaagcag atcgccgttc cggtcgtcac
      781 cggcgaaatg gaatggcttg attgtgactt cgaggacgag gtcacgttcc tccgtgatcg
      841 acggagcgga gaatacgtgg ccgttgtcat gatcacactt gggcccgaaa cctcctgggc
      901 gatcgattct gaccaagagt cccggggacg cggcgtggcc gtgtttcggt ttggcaagaa
      961 ccgctggata tccgatccca aggtttacat gaatctcacg ccagaggaga tggtgcgggc
     1021 attttcggac agaatggaac ttgtccgccg cgaaatgacg cgatagggcg aagcatcgtg
     1081 tagccctggg gtgtagttcg gccaagaggc cagcttcgtt gatgctatga ccctggctgg
     1141 ggcttttgac gcgatgccag ttgcttaaca agactttcga cttcttgcgg gttgaggtaa
     1201 atgttgacca gacgctgcat ctcggagaac attgcatcga tttgcttctg accaaccggg
     1261 tccggacaga agattctctg ttgctggcga gtcaattcac tcgaaaaaat ttcccgcggc
     1321 ttcagcttca gcaaattgtc gcgaatacgt tgggcggctt cccaatcttc cgcgatcatt
     1381 cgggcacgca ttcgcgcaat taagatggtt cgcaaagtga caagatccac gagttcgtct
     1441 tgaagacccg tgagataggt ttcggcaacg agtcgcgggt catcgttcgg aagggtcaac
     1501 tcagcaaggg aaccttcact aggaacgagt ggcactcgag ccagaagacg tcgaccatgc
     1561 ttgatcgaaa taagttgcca tgaggcgtcg cctgcgggga gcgcagcctg gccggcggca
     1621 tctgtacgtc ctacgaatat gggggacccc tcacccaagg gttgcgtgta gatttcatag
     1681 cctaccaaag gtccccgtgg agggtcacga ctctggagtc gcaatgtgac aggctgggct
     1741 cctgaaggtt gaacggcctg agcataaagt ttgacgcgac ctctccgccg ggcactcaac
     1801 gcgttgcgaa tgcccgaaac aacttcacac gtgattcccg taggttgctc agcgccaaca
     1861 tctgacgatc gtttgaggac cgtccaaggg agcggaatga cggcgcgagc gttgccttca
     1921 cggtcttcgt agcgggccac ggccacaaat aagtcgggaa catcgaggct gggcacacct
     1981 tcgtctgcct cgctcaagag gcctccacgg atggcgacat gcacggtccg ctcatcgacg
     2041 acttgaattt ctccgatggg aacgtgagca gacacaatac cctgggccac cacgtcgcat
     2101 aaacggctgc cggtggaagt ggacacaatg cgaagcggtc cccatcgttc caagcgtacg
     2161 tcccactctc gcgcctgaac ggtccaaccg acaccggttc ggattgtctg gaccacagtg
     2221 atcttgtcgg catcagtgat tttcttccac gaaggtggta accgatctcc cggcgggggt
     2281 gtccgtaaat cgagagatcg agagatgacc tcggtgggaa aaccagtcac ttcaaaatcc
     2341 cagcggttgc cgagcatagg acgtaacacg gcggccactt gtttatggaa ttggtggaca
     2401 tcgtcttcgg cggacaaaag acccggctcg aagtgaataa agaccttgac ccgatacggt
     2461 gaaaacaacc acgttacctg cgagtgagcg gagctggcta ggctaaataa gaaaatgccg
     2521 caaagcacga ggcgagggtc aaagcgtaac aaggcgtaat gagaaaaaat gaagcgaccc
     2581 ggccgcggag aagggctccc aaaccctttc gtcgattgag gaccaaccgc gatggtcgtt
     2641 cttggacatg caagggtcat tttcgtttca cactggcaca actgcttttg ccaaatgatc
     2701 catcaaaacg cccggggctg accgcctacc tcgttgtcat tccttggcga aacgcttatc
     2761 ggccgctgtc gctgagtgtc cacaattcca cgttttggta caaggtcgca cgttccgttt
     2821 cgatgccttg aatgcctttt cgaaacgcat aataatccgt aagttgccag agcggcaaca
     2881 cagttgtgtc ctcataaatg cgacggtgga gggtctttaa ccggcttgat gcgtctggcc
     2941 agtccaccgc ctgaccgagt tgactgagtg ccgcatctac ctgagtggga aagaactggc
     3001 tccgccggat caaggcaaaa cagcgggcaa ggtccgtgat ggggtcccac accgcggcct
     3061 ccacgtaaag aaaatcaaaa tcggatggta ggtcgggact tcctgggtga agttccacta
     3121 acgtggtttc ccagccaata agctgccact gtcgcttaat ctgcgaacac gcctctcggg
     3181 caatctcgtg tggtgggtgg acgaggatca gctttttccg agctggcacg ggcagaccgc
     3241 gattcttcgc aatctccacc gtttgtcgat aggccaaatc ggctaaggcc agggccagcg
     3301 acggctgata gggacgtgga gcaatttgcg tgtcgtatcc atggccgaga ggatcatcga
     3361 agcgacgtcc cacaggcagg ggtccgctga cgacttggca acctggcaga ttacgttgat
     3421 tgagaatcgc cttgagaatc cttgggcgat ctatggcgta gagcatcgca cggcgaaagt
     3481 cgcggttggc aggcaaaggc cgcgaaaaat tgggtaccag catatgcaca agtggaaccc
     3541 gataagatgc gatgctgata gaagcgtcgt tttcgagaag gtgcagttcc cagggattta
     3601 cccgatcgat catatccaca ttaccctgct tcagtgcccg gatggcatca cggcctcgag
     3661 aaaaggcgat ttcctcaatt tcgagtaacc cgggggcctg ttgacccgaa tgtgtgcgga
     3721 gatagacggc cgtcttatcg tctgattctt ctttggatcg cgtaaacggg ccgttttcgg
     3781 gcgtgagggc ctcacctgtg ccggtcaaat tccatcctgg agtgggaatc cagcggagaa
     3841 gtccttcggg gcgaacatgg gcatgtcgca attcgacgat gactacgtcg ggagagacca
     3901 tccgcacccg attaagtaat accgaccact cggaggtgaa gttctgatca acaggatcca
     3961 ccatggctag caactgatgg gccaggtcgt atgtggtata gacagaaccg gacgcatgaa
     4021 aaggcggtcg atccgggcga agttggaaaa caaggcgacg ccccaattcc tcgatcttcc
     4081 aatcgatgat ctgcgactga tagacgcccc cttcccctcc gggggcgacg tattccaaaa
     4141 gtgaacgata aagaagcctg cctgaccgtc gcgccgccca gtccgtgagg gaattggggt
     4201 cgaatgagat ggccggcaac gttacggcca cacggagccg cggataggct tcttgaacgc
     4261 gattcgagag ctcaggcaag ccagtcaatg cgggccaaat ctggcggagc tgacggagaa
     4321 ggcgatcggc ctgtcgtggt tggccggagg ccagtgccgc ttcaatctgg gtgcgtaagt
     4381 cctctgcttg ttgctgccaa accttttgcc accgctggac caagggatgt tggggcgtca
     4441 gttgtgcaag ggaatcgacg actcccctgg cacttgcgta atcggaggcc tcaagataac
     4501 ggagaatcaa tccttcggca gcctgggaga gatgatccgc cagacccgga tattgaggat
     4561 tcagacgata aaggttgagc aagttggcgt aggcgcggtc aaaagttttc gatgtcaaag
     4621 catccacggc ctcttcgtga agaaagagct gctgaacttc actcaagccc tctgccttgg
     4681 gataaagctg agataaatgg tcgagatagt catacgcctc accgaagcgt ttctcgcgaa
     4741 tgagttgcgt ggccttctca attagcagtc gttcaaaaag ccgaatttca acgatattcc
     4801 gccacggaag gcgataccgt tcgccaggtt catcgaaaag ttcgaggtca agatagcccg
     4861 tcttgggtaa tggctggggc agcttgcggt cgggcaaggc gagcggggcg accttgagta
     4921 cggcgtgatt gttggcggca tcgaggataa tcagatcgaa gggggcctga tcgtaaaggg
     4981 ggaccttctc gtcgatgagc gatttgatcg tctcggcaat ttgctcggcc ggggttcttg
     5041 ctcctgcgga cgacgaatct tttgcctgtt cagcatacaa ataaatgcaa acaaggaacg
     5101 agataatggc gagaatcacc gcctgtacgg cgtgcctttt gcgatggagt tggtggaaag
     5161 ttgtcagaag aag
//
LOCUS       DAOPTR010000119         5168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_13031_length_5168_cov_3.170155, whole genome shotgun sequence.
ACCESSION   DAOPTR010000119 DAOPTR010000000
VERSION     DAOPTR010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13031_length_5168_cov_3.170155"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /locus_tag="PKI05_03520"
     CDS             complement(<1..1391)
                     /locus_tag="PKI05_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOA51298.1"
                     /translation="MKSYLRKLAKIAFKAIGLLGLSLAFAYLPPILWGLWEIRLIHDL
                     EGQLAQVPPDQACLILEEFSEMGANGAPALVRALAHPDDTISSHAYRLLWRKLHSRET
                     QATGEQEALVIAEELHRISPFIPPSRKEHLAALLDQLLKWEDGGKNGFKIVKLCESLL
                     ERKLVDNRPVGEESKAAPGPNTAESSNGATVLRNSPPTMLQISGDPLPLRETGPNAEA
                     TASAVSPITEGMNTDHSSPAVVDSWQVSTSDLAAWREVFGGPLAQLAIYEKEVSTSEG
                     TSRSFGPGSMATYLAPQGTQDGGWKSVTREITPDRNETSAGPTLDESSSRPSSGKPSD
                     QPPRSISNRPDTTSAEKGATWATLAPLFAQLSGSPAEADRALQTLISLGITRPTIEVG
                     RIAFHPDSARRRQAIHLLWNLPGVDPLPFLLGLAQDSDPIVRREALAVLATTTNPAIH
                     SFVRDAIGRDSDP"
     gene            1825..2721
                     /locus_tag="PKI05_03525"
     CDS             1825..2721
                     /locus_tag="PKI05_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51299.1"
                     /translation="MSFFPILFILACLSVFAILPNFASGQGQYGASRGTSEAVSVSAK
                     EEKQQAINRIPLTELDPAWRTRVLDVAARAALFRRMSSQMIECEPEFYRFLVEHPEVV
                     VAMWQALGVTKLKLQIVGPQKYRLNDGAGTTAVVEYLYSSPNQHVVYSEGSYSGPLML
                     RPVEGRVLVVLTSNYLREATGTPYVSAQMDLFIQVDRAGIELITKALHPLLGGMAETN
                     YQQTMAFVGSVYRTAVVRPQSLERLASQLTQLEGNTQQDFLNWIARIGNDSGTTYDRA
                     AMITPPDGIHERALQPESTIRQ"
     gene            complement(2787..>5168)
                     /locus_tag="PKI05_03530"
     CDS             complement(2787..>5168)
                     /locus_tag="PKI05_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOA51300.1"
                     /translation="HERGRILAAYNFLSFTGMIMGSVIYWFLGSFLGLSARQMFVCAG
                     IVTFPVFLIILYLTPIDVIRVLFGWLMRWLYRFRVVGLENVPADGPAIIVSNHLSWLD
                     GMMIMLAMPRPIRMIAHLKYINAPIIRDLAKRAGVIAILPGAKSVALGIREARQALED
                     GELVGIFPEGGMTRTGQIRGFQPGFLKIARGLDVPIIPVYIDGIWGSIFTYSGGRYFW
                     KWPGLRPLRVTLYVGQPLRNIREAWQAHLALASLGAEVVSQKNTIDHIPPRRFLRTAR
                     SLLWGRKIADSTGIELTGGRLLIASLALRRFLRRHYLKPDETQVGVLLPSSVAGVVTN
                     AALALDRRIAVNLNYTLSEDLINYCIRSAGVKHVLTSRRMLERFPFKLEATVVFLEDI
                     YSQLELADKLISAFQAYLLPAWVLERLLGLHRIDPDDVLTLIFTSGSTGRPKGVMLTH
                     RNIGSNVAAFNQILHLRRSDVLCGILPFFHSFGYTTTLWTALQLKPMVVYHFSPLEPR
                     PIGQICRKYKATILVATPTFLRNYVRRCEPEDFAHLEVVITGAEKLPPEVADAFEQKF
                     GVRPWEGYGLTETSPVVSSNVPPSRVREPWFVSAKQGTVGRPIPGVAVKVVDVETGED
                     LGVGKQGMLLVKGPNVMKGYWNDPEQTAKVIKDGWYITGDLAQLDEDGFITITGRLSR
                     FSKIAGEMVPHLAVEEAIGRIEMTGDQETITFAVTGVPDEKKGERLVVLYTQLQHTPS
                     EICRALQSNGLPPLWVPDTEHFYRVDQIPVLGSGKLDLKALKEMAQQVAGGRD"
BASE COUNT         1182 a         1435 c         1429 g         1122 t
ORIGIN      
        1 tgaggatcgc tgtcgcggcc aatcgcatcg cggacaaatg aatggatcgc cgggttagtg
       61 gtggttgcca acaccgccag tgcctcccgt cggacaattg ggtctgaatc ttgggccagt
      121 ccgagaagaa aagggagtgg atccacgccg ggaaggttcc agagcaaatg gatcgcctga
      181 cggcggcggg cgctatcggg atgaaatgcg attctcccaa cctcaatggt aggccgagtt
      241 attcccaggg agatcaatgt ttgcagggca cgatcggctt ccgccggact gccagaaagt
      301 tgtgcaaaaa ggggcgcgag cgtcgcccag gtagcaccct tttctgcgga cgtggtatca
      361 gggcggttgc taatggacct gggaggctga tcacttggct ttcccgagct gggcctgcta
      421 gatgactcat cgagggtcgg tccggccgat gtttcattgc gatcaggtgt tatctctcga
      481 gtgacggatt tccaaccacc gtcttgggtg ccttgtgggg cgagatatgt ggccatgctg
      541 ccggggccga aagaccggga agttccttca gaagtgctaa cttctttctc gtaaatcgca
      601 agttgcgcca gaggaccacc gaacacctct ctccaggctg ccaaatcgct cgtcgaaact
      661 tgccaggagt caacaacggc aggagaagag tggtccgtgt tcattccttc tgttattgga
      721 gaaacagcgg aagccgtcgc ctccgcgttg gggcccgttt cgcgcaatgg gagggggtct
      781 cctgagattt ggagcattgt tggaggggaa tttctcagga cggtcgcacc gtttgacgat
      841 tcagccgtgt ttggaccggg cgctgccttg ctctcttctc caacgggcct gttatcaaca
      901 agttttcgct ccaacaaaga ttcgcacagt ttgacgatct taaagccgtt cttgcccccg
      961 tcctcccatt tgaggagttg atccaacagt gctgccaggt gttcttttcg tgacgggggg
     1021 ataaagggcg aaatacggtg caactcttcg gcgatcacca gagcttcttg ctcgccggtt
     1081 gcttgtgtct cgcgactgtg aagtttacgc caaaggagtc ggtatgcatg ggaagagata
     1141 gtgtcatcgg gatgagcgag tgctcgtacc aaagcgggcg ccccattagc tcccatttcg
     1201 gaaaactcct ctaggatcag gcaggcttga tcgggcggca cttgggcgag ctgcccctca
     1261 aggtcgtgaa taagcctaat ctcccaaagt ccccagagaa tgggcggtaa atatgcgaat
     1321 gccagcgaga gacccagaag gccaatcgct ttgaacgcaa tctttgctag ttttcttagg
     1381 tagcttttca cgtagcaaat accaccttct atgggttcgg ctggcccctc gatgggcacg
     1441 ctccccagtt tttaaaaaac ggacatccat caccgcgaga aagccaatct ttcggatgac
     1501 gaatccgcac aggttttgaa ccggagattc ggcccccgat cacattgtgg gccgaattgt
     1561 cccgcaattg cccgaatccg acaacactaa cgttacctat ttaagtcggt cctgaggtgg
     1621 gggtgctttt tttcaggttg cctgccggga attccgaata atggttagag cctacaaagg
     1681 aaacctgtaa tgcgcctctg tctgttcgcg cagcatcggc gaattgtcat ccaccggagt
     1741 tattgaagac tcgtgtccac atcgaacgac cgaccaccag aaaacttcca gccggtgagt
     1801 ctttcccggg aaaaattttc cagggtgtct ttcttcccga ttttatttat tcttgcctgt
     1861 ctttctgtgt tcgctatact gcccaatttt gccagcggcc agggacagta tggtgcttca
     1921 cgggggacct cggaggcggt ttcggtaagc gccaaagaag agaaacagca ggcaatcaac
     1981 cgcattcccc tgacggagct cgaccccgcc tggcggacga gagttcttga tgtcgcagcc
     2041 cgcgcggctc tgttccgccg aatgagttcg caaatgatcg aatgcgaacc tgagttctac
     2101 cgcttcctgg tggagcatcc agaagttgtc gtggccatgt ggcaggcctt gggcgtaacg
     2161 aagctgaaac tccagatcgt tggtccgcag aaataccggc tgaacgatgg ggcaggcacg
     2221 accgcagtgg tcgaatacct atactccagc cccaatcagc acgtcgttta ttccgagggc
     2281 agttacagtg gcccattgat gctccgtcca gtggaggggc gtgtccttgt tgtgctaacc
     2341 agcaattacc tgcgtgaagc tactgggaca ccttacgttt ccgcacaaat ggacctattt
     2401 attcaggtcg atcgtgcggg aatcgagctc atcaccaagg ccctgcaccc attgcttgga
     2461 ggaatggccg aaaccaatta ccaacaaacg atggcgtttg tcgggtctgt gtatcggaca
     2521 gccgtagtgc gaccgcagtc cttggagcga ctcgccagcc aattgaccca actggaagga
     2581 aacacacaac aagacttctt gaactggatc gcccgcatag gtaacgattc cggcaccacc
     2641 tacgaccgcg cggcgatgat taccccgccg gacggtatcc acgaacgggc gcttcagccg
     2701 gaatcgacaa ttcggcaata actgccggtt tccctcgggc atcttgcggc ggctttcctc
     2761 aggcgattgc ccgtctcact tgtaacctaa tctcggcccc cggcaacctg ctgcgccatt
     2821 tccttgagcg ctttgagatc gagcttacct gaccccaaca ccgggatctg atccactcgg
     2881 tagaagtgct cggtgtcggg gacccaaagc gggggaagcc cattgctttg caaggcccgg
     2941 catatttcag agggggtgtg ctgcaattgg gtgtaaagaa cgacaaggcg ctcccctttt
     3001 ttctcatcgg gaactcccgt cacagcaaag gttatcgtct cctgatcccc ggtcatctcg
     3061 atcctgccga tagcctcttc cacggccaga tgcggaacca tttcgccggc gatttttgaa
     3121 aagcggctta ggcgtccggt gatcgtgata aatccatcct catccaattg ggcaagatca
     3181 cccgtgatgt accaaccatc cttgatgacc ttggcggttt gctcagggtc gttccaatat
     3241 cccttcatga cgtttgggcc tttgaccaag agcatcccct gtttgccgac accgagatcc
     3301 tcgcccgttt ccacatccac aactttaacg gcaaccccgg ggatcgggcg tccgacggtt
     3361 ccctgctttg ccgagacaaa ccacggttcc cgaacacggg aaggcggcac attcgaggag
     3421 accacgggag aggtctccgt cagcccgtag ccttcccacg gacgcacgcc aaacttttgc
     3481 tcgaaggcat cggccacttc aggcggcagt ttctccgccc ccgtgatcac aacctcaaga
     3541 tgggcgaaat cctctggctc gcaacgtcgc acgtaattcc ttaagaatgt tggtgtcgct
     3601 acgaggatgg tcgccttata tttgcggcaa atctgtccaa tgggacgggg ttccaagggg
     3661 ctgaagtgat agaccaccat gggcttaagt tgaagtgctg tccacaaggt cgtcgtgtat
     3721 ccaaaagagt ggaaaaatgg caggataccg cagagaacgt cactgcgacg aaggtgcaga
     3781 atctggttaa atgccgccac gttcgaccca atgttgcgat gggtgagcat gactcccttg
     3841 ggacgacccg tggatccgga ggtgaaaatc agcgtgagta cgtcatccgg gtcgatacgg
     3901 tgaagaccga gcagccgttc caacacccag gccggtaaca gatacgcttg gaaagcgcta
     3961 ataagcttat cagccaactc cagttgcgaa tagatatctt cgagaaagac caccgttgcc
     4021 tcgagcttaa aggggaaacg ttccaacatc cgacggctgg tgagaacgtg cttaaccccc
     4081 gcggagcgga tgcaataatt aatcaagtcc tcggaaagtg tgtagttgag gttgacagca
     4141 atgcggcgat cgagggccag ggcagcattg gtcacaacgc cggccaccga ggagggtaaa
     4201 aggaccccaa cctgtgtctc atctggtttc aggtaatgac gtcgcaagaa acgtcgtagg
     4261 gccagcgagg caatgagaag tcgcccaccg gttagctcaa tgccggtaga atcggcgatc
     4321 ttccgtcccc acaataagga gcgagccgtc cgcaagaatc gccgcggcgg gatgtggtca
     4381 atcgtgttct tctgcgacac cacctcggct cctagcgacg ccagtgccag atgtgcttgc
     4441 catgcttccc ggatattcct cagcggttgc cccacataaa gcgtgacccg tagtggtcgc
     4501 aaacccggcc atttccaaaa gtagcgtccc ccggaatatg tgaagatact tccccagatg
     4561 ccatcaatat acacaggaat aattgggaca tcgagaccac gggcgatttt cagaaagcct
     4621 ggttggaagc cacgtatttg tcccgtccgg gtcatgccgc cctcggggaa gatgcccaca
     4681 agctctccgt cttccaaagc ttgccgggcc tcgcggattc ccaaggccac cgattttgcc
     4741 ccggggagaa tggcaatcac gccagcccgc tttgctaagt cgcggatgat cggggcatta
     4801 atgtacttaa ggtgggcaat catccgaatt ggccggggca tggccagcat gatcatcatg
     4861 ccatcgagcc aacttaagtg gttggaaacg ataatggccg ggccatctgc cggtacattt
     4921 tcgagcccca ccacgcgaaa tcgatacagc catcgcataa gccaaccgaa caagacccga
     4981 atcacatcga tgggggtcaa gtacaagatg atgagaaaga cgggaaacgt cacgatgccc
     5041 gcacaaacaa acatttgtcg agcgctaaga cccaggaaac tccccaggaa ccagtagatg
     5101 accgagccca tgatcatccc ggtaaagctc aaaaaattgt aggcagcaag gatgcggcct
     5161 cgctcgtg
//
LOCUS       DAOPTR010000120         5162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_13044_length_5162_cov_4.679655, whole genome shotgun sequence.
ACCESSION   DAOPTR010000120 DAOPTR010000000
VERSION     DAOPTR010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13044_length_5162_cov_4.679655"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            655..1383
                     /locus_tag="PKI05_03535"
     CDS             655..1383
                     /locus_tag="PKI05_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOA51301.1"
                     /translation="MTESKSSTQRAALRDPDVRLMLEVRNGNAAAFEELLLRYQSRVL
                     AVLENLLGNRHQAEDLTQEVFLRVYRSRHSYEPNAKFATWLFRIVNNVAMNALRSRCR
                     RREQTVPFQPGDSQHFDALDDAIQVSSGQIPARRLDKLEAREIVRMAMEGLNERQRLA
                     VLLCKFEGMSYADIAAAMKMSPKAVKSLLSRARTRLREILEPYFQSGQMPIGLGGTDD
                     VDSKRSTMIEDSQSDSQISLGGAG"
     gene            1491..3401
                     /locus_tag="PKI05_03540"
     CDS             1491..3401
                     /locus_tag="PKI05_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51302.1"
                     /translation="MSDSAFQKRSDISIDAIREDLVAYLDGELSSEEAARIERLVEQD
                     TRIRSELQRLAETWDWLDVLDRPTVDEDFTRTTIEMVAQSAASEVVAQTQTKWKRQAI
                     VLGVAVLGTLLAVWGGWQLASFLWENPNKWLIEHYAVIQNQDYYRHIENVAFLRMLRD
                     SGLYTDEEEYLNPDDAQEVDEWRMFRPPGPPGPGQGQRGPAGAGEIPGEGGPTSNLRP
                     EPRDNRPSQVPRQPLSESSGEKLPSGSTAVVTSKPVSYPASSSVITLSDPAEIRKHIA
                     GLTDAEKVELRRKIERFQQLSPSEQARMKAIHEELMQAPDRDELIAVMQRYYEWFKLL
                     TPSQRGELETLEPAQRISWIQKTRNEMLTAAFSRALFNFPPERLRTLLQDSMRVPPEE
                     RLPPGDVAVLVRFVESLAAERGEELLAHLNSGEKQDLLRRLSRVTDPQQRRDFLAITW
                     FQWQLDHPEQASALTPQELEELRKQFSPGTRARLESLSTEEQLKRVAQWIRSYVFFKY
                     VMPRVWADMRRAVSEQELGRFVEENLTREEREKLLALPPDEMQRELTRRYLRWRYPGF
                     DTLGPWRGHGGERRGPRPGPGEREPPPGPGGSSDRDRGDGPPPQGPGANDVSTREPLR
                     PMPLLPPPGEVP"
     gene            3562..4602
                     /locus_tag="PKI05_03545"
     CDS             3562..4602
                     /locus_tag="PKI05_03545"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOA51303.1"
                     /translation="MKIGLVGYQGCGKSTLFEWLTGVPPDPAQAFSGQSAMATIPDPR
                     LQELERIYNAKKVTPAALEIVDTPGLSRTHQGNAAKLAILRDVGCLVMVVPAFGGFDC
                     AAEFRSFQEDLIIADLEIVTGRIERVEASLKKPHPKAEREHLEEELKTLQWVKQRLEE
                     NNPIREAEMTPEQQRVTRAFRLLCEKPQMVVVNTADDERDFSRFRGMFPEDVPFFAAP
                     LALELELSKMSPEEREQFQAELGVGGADRGALIRTIMDVAGLHVFLTAGPKEVRAWLL
                     RKGGTAVEAAEAIHTDLAKGFIRAEVMSCQDLIRLGSEREMKAQNLVRHEHRDYVVQD
                     DDILLIRFSVDR"
     gene            complement(4672..>5162)
                     /locus_tag="PKI05_03550"
     CDS             complement(4672..>5162)
                     /locus_tag="PKI05_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51304.1"
                     /translation="PKKVVDEYIRDGKPSDPNNPNSPPFFRKLRDYESAIRYLHLKRS
                     ELMDQVAARTADKGRLEAAVADSQREAKARDQEVAQLQEVLKRAEFERDLAVNHLKAV
                     EDTLARVTVARDALIQQNRVTAAEIAQAQEKARQLIEQRVRSVAQSEQPASSVTAEGT
                     RR"
BASE COUNT         1170 a         1349 c         1534 g         1109 t
ORIGIN      
        1 caccaaagcg acagctcaac cggatcgctg ggcatggcgt gttccctcat ttacctacga
       61 cgccccgccg aaatcccggt ttgacgcatc cctgaagggg cttggtaaca cgggcaatgt
      121 catagcgtca cggctccacc cttttaaagc cacctcgagc gacggcaacg aaaatagcag
      181 atatgccgat tttgcccctt cttttcactt tgattcctgt cgaggtaaaa ggcaacaatt
      241 cggcgctgtc ttggacgcag acatccccct ttcattgggg gtaaggtacg ctcagaaccc
      301 aacgacactt ttggggttaa aactcataga gtgacgaggg tggaaagatt agccgcaagg
      361 tcccaccgcg agcagtggct ctggcagctg gggcaagaga gggaatcgtg gtagttcagc
      421 gggctggtac tacgattcgt ggaaaacctg tatccgtgtt tttagtcgct gcacgcggga
      481 aaaattaacc ttggcccgaa gatcggcacg ttagtgggtc gctccggtgg tcggatgtgg
      541 aatcttgtcc ggtgagatgc catcatagag agttgatagt actccaaaga tgtccagaat
      601 gtatgatgga cagaggctaa tactcaccgg ctcgattcca cacgaggata gcgcttgacg
      661 gaaagcaaat cgtccaccca aagggccgca cttcgtgacc ctgatgtgcg attgatgtta
      721 gaggtccgca acggcaacgc ggcggcgttt gaggaattgt tgttgcgcta tcagagtcgg
      781 gtcctggcag tgctggaaaa cttgttggga aatcgacatc aggccgagga ccttacccag
      841 gaagtttttc tgagggtcta tcgatcgcgc cacagctatg agcccaatgc caagtttgcc
      901 acttggcttt ttaggattgt caacaatgtg gccatgaatg cattgcgaag tcggtgccga
      961 aggcgagaac aaacggttcc gtttcagcca ggcgattctc agcattttga cgcccttgac
     1021 gatgccatcc aggtttccag tggtcaaatc ccggcccgcc ggctggataa attggaagcg
     1081 cgagaaatcg tccgcatggc gatggagggg ctgaatgaac ggcagcggtt ggccgtgttg
     1141 ctctgtaagt tcgagggcat gagttacgca gacatcgctg ccgccatgaa aatgtcaccc
     1201 aaggcagtaa agtcgctcct ttcgcgagcg cggacacgtt tgcgggagat cctagaacct
     1261 tacttccaaa gcggtcaaat gcccattgga ttggggggca ccgacgacgt tgactcgaaa
     1321 cggagcacca tgattgaaga ctcgcaaagt gattctcaaa ttagcttggg cggagccgga
     1381 taagatcagg ttgaggccaa aaaaattgtg gacgttttca acataggtcc acgtcaccac
     1441 gtcgatggtc gagaggcgga tgatcatgtc aatatggcga gttacgggcg atgagcgatt
     1501 cggcatttca aaaacgttcc gatatttcga ttgacgccat ccgcgaggat ctcgtcgcgt
     1561 acctcgatgg tgaattatca tccgaggagg ctgcccgaat cgaacggctc gtggaacagg
     1621 atacccggat tcgttcagaa cttcagcgtt tggccgagac ttgggattgg ctggatgtgc
     1681 tggaccgccc gaccgtggat gaagatttta cccgaaccac gatcgagatg gtggctcagt
     1741 ctgcggcttc cgaagtcgtg gctcaaacgc agactaaatg gaaacgccag gcaattgttt
     1801 taggtgtggc ggtcttgggt acattattgg ccgtttgggg tggctggcaa ttggcgtctt
     1861 tcctctggga aaaccccaac aaatggctaa tcgagcatta tgcggtcata caaaaccagg
     1921 attattaccg ccatattgaa aatgtggcct tcctgcgaat gcttcgcgac agcggcctat
     1981 atacggacga agaagagtac ctcaatccgg acgacgccca agaggttgat gaatggcgga
     2041 tgttccgacc gcctggtccg cccggccccg ggcaaggcca aaggggaccg gctggtgccg
     2101 gagaaatacc cggcgaaggg ggaccgactt caaatctgag acccgagcct cgggataatc
     2161 gaccgtccca ggttcctcga caaccgttat ctgagagttc gggagagaag ctaccttccg
     2221 gttcgacggc cgtggtgacg tcaaagcctg tttcctatcc cgcgtcatcc tctgttatca
     2281 cgttgtcgga ccccgccgag attcgcaaac acatcgcggg actcacggat gcggaaaagg
     2341 tcgaattgcg tcggaaaatt gaaagattcc agcagttgtc gccaagtgaa caagcacgta
     2401 tgaaagccat tcacgaggag ctgatgcagg ctccagatcg cgacgaattg attgcggtta
     2461 tgcagcggta ctacgagtgg ttcaagcttt tgacacctag ccagcgaggc gagttagaga
     2521 ctctggaacc ggcgcaacga ataagctgga ttcagaagac ccgcaacgag atgttgaccg
     2581 cggcgttcag tcgggcctta ttcaattttc cacccgagcg attgagaacg cttctccaag
     2641 actcgatgag ggttccacca gaggagcgtt tgccgccggg agacgtcgct gtcctggtgc
     2701 gttttgtgga gagcttggcc gcggagcggg gagaagaatt gctagcccat ttgaacagcg
     2761 gagaaaaaca ggacttactc cggagactca gtcgtgtgac ggacccgcaa cagcgaagag
     2821 actttttggc catcacgtgg ttccaatggc aactggatca ccccgagcag gcctcggctt
     2881 tgacaccaca agaattagag gagttacgga agcaattttc gcctggcaca cgggctcggc
     2941 ttgaatctct ttccacggaa gaacaattga agcgtgtcgc ccaatggatt cgcagctacg
     3001 ttttcttcaa gtacgtcatg ccgcgggtgt gggcggatat gcgacgggcg gtcagtgaac
     3061 aggagttggg ccggtttgtc gaggagaatt tgacgcgcga ggaacgagag aagctcttag
     3121 cgctgccacc tgacgagatg cagcgcgaac tgacccgccg ctatctgcga tggcgctacc
     3181 cgggctttga caccctagga ccgtggcgcg gacacggtgg cgagcgccgc ggaccgcgac
     3241 cgggaccggg agaacgagaa ccgccgccag gccccggcgg ctcatctgat cgagatcgag
     3301 gcgatggccc acctccgcag gggccaggag ccaatgatgt atccacgaga gagcctcttc
     3361 gcccaatgcc tctcttacca ccgcctggtg aagtgccata atagagggaa gtgacgaacc
     3421 ctttcggggc gatttgctcg atcttgcgtt gcgatccatc gccgtccacc tgccgcgcgc
     3481 atcgttgtta acgaccagtg caaagatcgc ttgaacatag ctggtggcac gctcggtgtg
     3541 gccgtttggg agtcttaact aatgaagatc ggacttgtag gttaccaagg atgcggaaag
     3601 agtacgcttt tcgagtggct gacaggcgtc ccaccggacc cggcacaggc gttttccggg
     3661 caatccgcca tggccacgat tcccgatccg cggttgcagg agttggagcg gatttataac
     3721 gcaaagaagg tgacaccggc cgccttagag atcgtcgata cgccgggcct gagccgcaca
     3781 caccagggca atgcagcaaa actggcaatc cttcgggacg tcggatgttt ggtaatggtt
     3841 gtgccagctt ttggcgggtt cgattgcgca gctgagttcc ggtcctttca ggaagacctg
     3901 atcatcgccg atttggaaat cgtgacaggc cgcatcgaac gggtggaggc ctccctgaaa
     3961 aagccccatc cgaaagcgga acgcgagcac ctggaagagg aactcaagac tcttcagtgg
     4021 gttaaacagc gactggagga aaacaatcca attcgggaag ctgaaatgac tcccgaacaa
     4081 caacgtgtca caagggcctt ccggcttctt tgtgaaaaac cgcagatggt cgtggtgaac
     4141 acggctgacg atgagcgcga tttctcgcgt tttcgtggga tgtttcccga ggacgtgccc
     4201 ttttttgctg cgcccttggc cttagagctg gaactgtcta agatgtcgcc cgaagaacgg
     4261 gaacaattcc aggcagaact cggggtggga ggggccgatc ggggggccct catccggacc
     4321 atcatggatg tggccgggtt acatgtgttt ctcacagcgg ggccaaaaga agtccgcgcg
     4381 tggctcctcc gcaagggtgg aacggccgtg gaggccgccg aggccattca caccgatctc
     4441 gctaagggtt tcattcgtgc cgaggtaatg tcctgccagg acttaattcg attgggaagt
     4501 gaaagggaaa tgaaggcgca gaacctggtg cgtcacgagc accgcgatta tgtcgtccaa
     4561 gacgacgaca tcctcctcat tcgtttcagt gtggaccgat aaaattcgcg gttacttcag
     4621 gacgaatgcc gacgggcgat aaggtttcca ggaggagccg gcgactcgct gttagcgccg
     4681 cgttccctct gccgttacgg acgaggccgg ctgttcactt tgtgccacgc tgcggacacg
     4741 ctgttcgata agttgtcggg ccttctcttg cgcttgggcg atctccgccg ctgtgacccg
     4801 attttgctgg atgagggcat cgcgagcgac ggtcacacga gcgagtgtgt cttcaaccgc
     4861 ctttagatga tttaccgcca aatcccgttc gaactcggcc cgcttcagta cttcttgcaa
     4921 ttgcgccact tcctgatcgc gggccttggc ttcgcgctgc gagtcagcaa cggctgcctc
     4981 cagtcgaccc ttgtctgccg tgcgggcagc aacctgatcc atgagttcgc tccgtttcaa
     5041 atgaaggtag cgaatggccg actcataatc tcggagcttt cggaaaaacg gcggtgagtt
     5101 ggggttatta ggatcacttg gtttgccatc tcgaatgtac tcatccacga ccttcttggg
     5161 ta
//
LOCUS       DAOPTR010000121         5151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_13081_length_5151_cov_3.480377, whole genome shotgun sequence.
ACCESSION   DAOPTR010000121 DAOPTR010000000
VERSION     DAOPTR010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13081_length_5151_cov_3.480377"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            239..2083
                     /locus_tag="PKI05_03555"
     CDS             239..2083
                     /locus_tag="PKI05_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51305.1"
                     /translation="MTMSVRIYLLVTILLIIGRSPNIVALERPAEPVYEVVSPLGERV
                     VEAGAMAPRLETLNGKTICMVWNHAFKADVTLPAIAEELKKRFPDIKIVPYYSMPLAP
                     LPELPGSPTKESENLRAALKAMGADAVIVGNGGCGICTPRACYATVVAEQLGIPAVVV
                     TNPGFDEQARTMAQYSGIPSLQVAVYPDPFDLETEAQLVEKSKTILVPQIIDALTKSI
                     AAAKDSVKIDSRKIVFTGTLDEINRYFTALGWSDGLSISPPTVDRIEEFLKYTDYGPH
                     EEIAVLPPANLKATPWTIAANGVMAGCRPEYMPILIAAVQAMGDPAFRYRSHGASTHS
                     ITSFFWVNGPLARQLGIDHGQGLIAHPVNAAIGRAMSLIERNIAGLRIKETQMGSFGK
                     MMSWVLAEDEQAMIQWGWEPYHVEKGFDRNANTITAGTSTVWGRNLVPATSEPEIITQ
                     LIAYDAVNTEAFASGFTGQSRRYVLIGPGVAEILAGGGYTKGTLRRDIMRMGRKITYE
                     AVFSQVYGSFGRVQRSFEEELRRALRYSEKGKLPPWYPRFPGWEEIETVPAVGMIEFI
                     VCGDPIRNKVQILAGGPGIASKEIKLPANWDELMQKAGYRPLREFYLQ"
     gene            3167..>5151
                     /locus_tag="PKI05_03560"
     CDS             3167..>5151
                     /locus_tag="PKI05_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51306.1"
                     /translation="MSRSLKRCVGVLFLFSLGWVGHNLLAEQAGISPWSKNPYYWQYN
                     GKPVMLIGGSKDDNLFQIPDLKEHLDEMVAVGANYIRNTMSDRPDKGFEVYPFKKLEN
                     GKYDLNQWNDEYWQRFENLLKWTKERNIFVQIEVWDRFDYSRDNWEPHPYNPKNNINY
                     TYEESGFAEHYPDHPGSNKQPFFFTTPYQRNNKVVFPYQERFVDKMLSYSLQYDHVLY
                     CIDNETSGEEEWAKYWAEHIRRKAAQIGKKVCVTEMWDDWDLKAPRHRRTLDHPELYA
                     FADISQNNHQKGQTHWDNFQWVRDYVKNQPRPLNSVKIYGADTGRYGNDQDGVERFWR
                     LLLGGAAAVRFHRPDSGLGLNDKAKASIQAARKVESLVPWWELRPAMELLGNREANEA
                     YAAAKPGEACVVFFCNGGEVTVNLSQRAAGEAKLRWINVSDGQWGPEGTTKAGGLVTL
                     KTPQAGPWVAVILRNSPAVAARQSGSSDVKRSVPATLISWAEKAPQKQTAHGVLRVCP
                     ANPRYFTDDSGKAILMVGSHTWNNLVDMGPGDPPPAFDFPAYLDFLEKYNHNFIRMWT
                     WELLTWDTAGNREPKAQVHVAFPHPWKRTGPGTARDGKPKFDLTQFDENYFSRLRERV
                     KLAQARGIYVSIMLFEGWGLQFSPNAYESHPFHPDNN"
BASE COUNT         1138 a         1382 c         1479 g         1152 t
ORIGIN      
        1 cgacagacaa ttccacgatg gccccctcgc tcaggatagc ggcgatgagc tgcttcgact
       61 tcgagatcat cggaacctag agttccgaaa agggttatat cttcaagaga gaaaacgtca
      121 ccaaattatg ttcggggctt ctgttcgggg ttccccggtt ggatcgggcg cctgctgatc
      181 gtgagttggg ggccaaaact gcggtcacga aaatggacgt ttagggaggg ttcctcaaat
      241 gaccatgtca gtgcgaatct atcttctcgt gacaatccta ttgattatcg gtcggtcgcc
      301 gaacatcgtg gccttggagc gtcctgccga gcctgtgtac gaggtcgtga gtcctttggg
      361 ggagcgggtt gttgaggcgg gggccatggc ccctaggttg gaaacgctca acggcaagac
      421 gatctgcatg gtatggaatc atgcgttcaa agcggatgtg actctcccgg ctattgccga
      481 ggaactgaaa aaacggtttc ccgacataaa aattgtcccc tattattcca tgcctctagc
      541 tcccttgccg gaacttcctg gatcacccac gaaagagtcc gagaatctgc gagcggcttt
      601 gaaggccatg ggcgctgatg ccgtgattgt gggaaatggc ggctgcggca tttgcacgcc
      661 ccgcgcgtgc tatgccacgg tggtggccga gcagctcggc atccccgccg ttgtggtgac
      721 aaatcctggc ttcgatgaac aagcccggac catggcccaa tactcgggca ttccgtcgct
      781 ccaggtcgcc gtatatcccg atccttttga tctggaaacg gaggcgcaac tggtcgagaa
      841 atcgaagacg atcctcgtgc ctcagattat tgatgctttg acaaaatcga tcgcggcggc
      901 aaaagattct gttaaaatcg atagccggaa gatcgtattt actgggacac tcgatgagat
      961 taatcgctat ttcacggccc tcggctggag tgatggatta tccatttcgc cgccgaccgt
     1021 ggatcgaata gaggaatttc tcaaatacac ggattatggc cctcacgaag agatcgcggt
     1081 cctaccgcca gcaaatctca aggcgacacc gtggacaatc gccgccaatg gtgtcatggc
     1141 cggttgccgt ccggagtaca tgccgattct aattgccgcg gtgcaggcga tgggtgatcc
     1201 tgctttccgg tacagaagtc atggggccag tacccattcg atcacaagtt tcttttgggt
     1261 caacgggccc cttgcgagac aactcggcat tgatcatggg cagggactca ttgcccatcc
     1321 cgtcaacgct gccattggac gtgccatgag cctcattgag aggaatatcg cagggctaag
     1381 aattaaggaa acccaaatgg gtagcttcgg caagatgatg tcgtgggtcc tggcggaaga
     1441 cgaacaggct atgatccagt gggggtggga accgtaccat gttgagaagg gatttgatag
     1501 aaatgctaat accatcactg cgggcaccag cacggtttgg ggccgaaatc ttgtgcccgc
     1561 gacatccgag cccgaaataa ttacccaact catcgcctat gacgctgtca acacggaggc
     1621 ctttgccagc ggctttacgg gtcaaagtcg gcgctatgtg ctaattggac ccggcgttgc
     1681 cgaaatcctt gccggtggag gttacacaaa gggtaccttg aggcgagata tcatgagaat
     1741 gggtcgaaag atcacttacg aggcggtctt ttcccaggtc tatggctcgt ttggccgagt
     1801 gcaacgttcc ttcgaggagg aacttcgacg ggcgttgcgc tacagcgaaa aagggaaact
     1861 gccgccgtgg taccctcgat tccccggttg ggaagagatt gaaaccgtgc cggccgtggg
     1921 catgattgaa ttcatcgtct gcggagatcc tatcaggaat aaggttcaaa tcctcgccgg
     1981 tggcccgggc atcgcgagca aagaaatcaa attgcccgcc aactgggatg aactcatgca
     2041 gaaagcagga taccgtccgc tgcgagagtt ctatcttcag tagcgatggt ttacggaaaa
     2101 gccgttggcg gccagtttcc ggtagtgcgt tggatctgtc agcctgtgtc tgagttcgcg
     2161 agctaaatac ctccacctgg gcagttcagt gccctaactg gcgagctttt acccatcgcc
     2221 cgaaaactct cggtttgccg ggaaaaatcg ccgtgttgtt ccacccagcg ttgccttttt
     2281 tcaaggattg cctgtatcgg gagggtcgcc gtcctcggcg accgctggat ctattgcatc
     2341 tatcctcggc gaccgcgcga ggatcagcgt ctgcgatgac ccaatcaccg gacggcgtgg
     2401 acgccgtccc tcccggggct acactatcat cgacgcctgt caagtggcgt acgggggccg
     2461 ttaggaatgg cgtcccgctc cttcgattcg gcgaaacggt caggtaccgt ttctcgtaat
     2521 tgtccgccgt agtcaccttc gcgctctgtc gcgtctgccg tgtggtctcg cttgtttgtc
     2581 ctgtccgttg ccaatctggg agggacgcgg tccccggcgt cctttggtac tagtttggcg
     2641 tgcgtcatgg tcgttccctg tagttgcggc agacacacgg gcctagggca ggcacggggg
     2701 cctgccccta cggtttcaat cggtgcgtgt aggggcaacc ccccgtggtt gcccctttat
     2761 gcgcagcgcc cagggttcgc gtttatgcgc tcattcgttg tttgaatttg acggggaccc
     2821 gggcgctggt tacgttctgg ggcctgcccc tacggtttcg atggaatgcg cgtacgggca
     2881 accccctgtg gttgctcacc gtggtcactt tcgcctggtg gcacgccccg cccccacgag
     2941 aatctgttga gacaggcaat aaccgtgagg cagatggacc agactgccgc cgtgttttat
     3001 taggggcagc acatggggcg ttatggttgg gcagtgcttc cctcttctca acgggcgaca
     3061 atcttagcca aagggttgct tttgcttgtg gctgtccaca taatagggca cacctgatga
     3121 gttccacatc gttttccctc aactcaccta tgtcgggagg caaatcatga gtcgttcact
     3181 gaagcgatgt gtcggggtgt tgtttttgtt ttctcttggg tgggtaggtc acaacctact
     3241 ggccgaacag gcgggcatct ctccttggtc gaagaacccc tattattggc agtacaatgg
     3301 caagcccgtc atgctcattg ggggaagcaa agatgacaac ctcttccaga ttcccgatct
     3361 gaaagagcac ctcgacgaga tggtagccgt cggggccaac tacattcgga atacgatgag
     3421 cgatcggcca gacaagggct ttgaggtcta tcctttcaaa aagctagaga atggcaaata
     3481 cgacctgaac cagtggaacg acgaatactg gcagcgattt gagaatcttc ttaagtggac
     3541 gaaggagcgc aacatcttcg tacaaatcga ggtttgggat cgattcgatt actcgcggga
     3601 caattgggag ccccatcctt ataaccccaa aaacaatatc aactacacct acgaggaatc
     3661 gggatttgcg gaacattatc ccgaccaccc gggcagcaac aaacagccct tctttttcac
     3721 cacgccctat caacgtaaca acaaggttgt gttcccttat caagagcggt tcgtcgataa
     3781 gatgctctcc tattccctgc aatacgatca cgtgctttac tgcatcgaca atgagacgtc
     3841 gggcgaagaa gaatgggcaa aatactgggc ggagcatatc cgacgaaagg ccgcacagat
     3901 cggtaagaag gtctgcgtga ccgaaatgtg ggacgactgg gatcttaaag cgccgaggca
     3961 tcggcgaacg ctggaccatc ccgaacttta tgcatttgcc gacatctctc agaacaatca
     4021 tcaaaagggg caaacgcact gggataattt ccagtgggtt cgggattatg tgaaaaatca
     4081 accgcggcct ctcaacagcg tcaaaatcta cggggccgat acaggtcgtt acgggaacga
     4141 tcaagatggc gtcgagcggt tctggcgact ccttctcggg ggcgccgcgg cggtccgttt
     4201 ccaccggcca gactctggat tgggcctgaa cgacaaagcg aaggcttcga ttcaggccgc
     4261 gcgaaaggtg gaatcccttg tcccctggtg ggaacttcgg ccggccatgg aactgctcgg
     4321 caatcgtgaa gccaacgagg cctatgcggc ggccaaaccg ggcgaggcgt gtgtggtctt
     4381 cttctgcaat gggggcgagg tgaccgtgaa cctctcccag cgggcggcgg gagaagcgaa
     4441 actccgttgg ataaacgtct ccgatggtca gtgggggcca gaaggaacaa cgaaggccgg
     4501 cggcctcgta acgctcaaga cgccccaggc cggaccgtgg gttgccgtta ttctccggaa
     4561 ctccccagca gtggcggccc ggcagagtgg gtcaagcgat gtgaaacgct ctgtcccggc
     4621 aacgctgatt tcctgggcag aaaaagcccc ccaaaaacaa accgctcacg gtgttttgcg
     4681 cgtttgcccg gcgaatccgc gttacttcac cgacgactcg ggaaaggcga ttctcatggt
     4741 aggttcacac acatggaaca atttggttga catgggaccg ggtgatccgc cgccggcgtt
     4801 cgattttccg gcctacctgg actttctcga aaagtacaat cacaacttca ttcgcatgtg
     4861 gacgtgggaa ctgctcacct gggacacggc aggcaaccgt gaaccaaagg cccaggtgca
     4921 cgtcgccttt ccgcatccgt ggaaacgaac cggtcccggc actgctcgtg atggcaagcc
     4981 aaagtttgac ctgacacagt tcgatgaaaa ctatttttcg cggcttcggg aacgtgttaa
     5041 gttggcccag gcaaggggaa tctacgtctc aatcatgctt tttgagggtt ggggccttca
     5101 attctcacca aacgcctacg aatcgcatcc ttttcatcct gacaacaaca t
//
LOCUS       DAOPTR010000122         5121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_13172_length_5121_cov_3.058429, whole genome shotgun sequence.
ACCESSION   DAOPTR010000122 DAOPTR010000000
VERSION     DAOPTR010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13172_length_5121_cov_3.058429"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="PKI05_03565"
     CDS             complement(<1..731)
                     /locus_tag="PKI05_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOA51307.1"
                     /translation="MNLTKRQTISFGSKAGMSIAPWGAGQSGGFLALDRKWRPTRGLT
                     LLEVLLSLGLIAILLAALGTAVDFHYRVVQSARHQVEETQLARVLLDRIAQDIRGAVS
                     YDPVNFEELIPGLVTKSGSSSNLSSLAEEAGIDPSLLLESGNEMGTYSTGGDWTSTTL
                     PRSVPGLYGGSNWIEIDVSRLPRPDQWQSEYALAADGLLVDRISDVKTVAYFVLSPEE
                     TINYSFVSMPGVVGFDQQGGLVRREL"
     gene            complement(811..1353)
                     /locus_tag="PKI05_03570"
     CDS             complement(811..1353)
                     /locus_tag="PKI05_03570"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOA51308.1"
                     /translation="MFTCEKKLPTSNKPDRHRRDGFTLLEVLLALAILLTSLAAIGQL
                     VFNAYQNALRVKNLTRAQLICEGILAELNAGSLPLETIADAPVEDIDDTVEPGWLYSI
                     DIGELDTNGLLSVRVTVYQDPSLFARPVELTLTQWMIDPTVEFVQPGETAGSEGMESS
                     ATSESAPSGESTGQEGTGSR"
     gene            complement(1415..2137)
                     /locus_tag="PKI05_03575"
     CDS             complement(1415..2137)
                     /locus_tag="PKI05_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51309.1"
                     /translation="MSLVFGRTYAARKVQWTVVPPGRSKRACGDVPRLAFTLLEVVIV
                     LVLVVILSGVAFNALMRPMAARRLQLAADQIRAEWIRARNRAMANGQTYYFQCELGGN
                     GYVLVPYDASVPGTDVYDPSMGTQDMTDVPLMQIPGGGSRKELPEKITFVDVQVEGGE
                     LTAAITEDPTLSSPPSDGSSILFFPDGTTSSALITLENEFGRRIDISLRGLTGAVLVG
                     ELYDSESAGMTELPAAPSVAGS"
     gene            complement(2186..2638)
                     /gene="gspG"
                     /locus_tag="PKI05_03580"
     CDS             complement(2186..2638)
                     /gene="gspG"
                     /locus_tag="PKI05_03580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOA51310.1"
                     /translation="MRQTKGFVRKKLSRTTRKGLTLIEVLIVLTILLIIASLAVTNLI
                     AVQRRAYINAARTQIKAFEQPLHAYYLDFNEYPPSLDALITPPADLADPTKWNGPYLD
                     AQAVPLDPWGREYQYLYPGQYNTDKPDIWSMGPDGVDGTEDDITNWGQ"
     gene            complement(3114..4325)
                     /locus_tag="PKI05_03585"
     CDS             complement(3114..4325)
                     /locus_tag="PKI05_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOA51311.1"
                     /translation="MPEFSYVAVDANGQRVTGTIAAATKREALTAIASRELYPLEVRS
                     EAPARPLFSFGRRSVPAQAMAVVYGQLADLLRSGVPLLRALNIVKSQSVHPTLKEVLG
                     EVARRVEDGMGLGDAMARFPGVFSEMASSMVRAGSEGGFLEEALYRVAEFTEATEDLR
                     KRVKGAMAYPMFLIVIGSLLVSGLLIFLVPQFEGMFQNLRERNELPGLTEGLLAFSQF
                     LQAWGIWVVVVIILGFFSVRAWLRSETGRRLWDRYVLKVPILGGIVLNFAVARFCRVL
                     GTLLKNGVPILRALEIASEATGNVLLGEAVHKATKNISQGQRLAEPLAACKLFPPLVV
                     EMIAVAEEANTLETVLLQIADSLERRTWRQLDVGVRLLEPILLLLVAMAVLVVVVALL
                     LPVFKMSGAVG"
     gene            complement(4400..>5121)
                     /locus_tag="PKI05_03590"
     CDS             complement(4400..>5121)
                     /locus_tag="PKI05_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOA51312.1"
                     /translation="KTTTLYSALLEIRDESIKIITTEDPVEYQLEGINQIQVHPKIGL
                     TFAHSLRSILRHDPDVILVGEIRDLETAANAIQASLTGHLVFSTLHTNDAAGAYTRLV
                     DMGVEPFLVSSTVEAVMAQRLVRTLCPECKEPYRPRPEELPKDFPREILEQGVTLYRA
                     VGCRACRHVGYRGRVGIFELLVTSERIRELAHDRASSWVIRQTAIEEGMITLRQDAWR
                     KVLSGRTTIEEIERVTRAEEH"
BASE COUNT         1118 a         1626 c         1336 g         1041 t
ORIGIN      
        1 agttcccgcc gcaccaatcc cccctgctgg tcaaacccaa cgactcccgg catgctcacg
       61 aacgagtagt tgatggtctc ctccggcgag aggacgaaat acgcaaccgt cttcacgtcg
      121 ctgatgcggt ccaccaaaag accatccgcc gcgagggcat attcggattg ccattgatcg
      181 ggccggggca aacggctaac gtcaatctcg atccagtttg agcccccata taaccccggg
      241 acagaacgtg gcagcgttgt tgatgtccag tcgcctcccg tactatatgt ccccatctcg
      301 ttacccgatt caagaagcag actgggatca attccagcct cctcggccag cgaggaaagg
      361 ttgctactgc tccccgactt tgtgaccaga cccgggatca attcctcaaa attgacagga
      421 tcatacgaga ccgccccgcg gatatcctgg gcaatccgat cgagcaggac cctcgccaat
      481 tgtgtctctt ccacctgatg ccgggcagac tgaaccaccc gataatgaaa atccaccgcc
      541 gtgcccaggg cagccaacaa gatggcgatt aaaccgagtg aaaggagtac ctccaagagc
      601 gtcagaccac gggtcgggcg ccacttccga tccagggcga gaaaaccacc gctttgcccc
      661 gctccccagg gcgcgatgct cattcccgct ttgcttccga agctgattgt ctgtcgcttg
      721 gtaagattca tatgtcgcct tccgtaccgt ctgatggcca cacttcactc ggctcctaaa
      781 taccgctctg cccgttcgtt caccgcattt ttaccgtgag cccgtgcctt cctggcctgt
      841 cgattctccc gacggagcag attccgaagt cgcggagctt tccatgccct cgctccccgc
      901 ggtttcaccc ggctgcacga actccacggt ggggtcaatc atccactggg tcagcgtcag
      961 ttcaacaggc cgagcaaaca gcgaggggtc ctgatagacc gtcacccgaa cgctcaaaag
     1021 cccgttggtg tcaagttccc cgatgtcaat tgagtaaagc cagcccggct caaccgtgtc
     1081 gtcaatatct tccaccggtg catcggcaat cgtttcaagc ggcaaggaac cggcgttcag
     1141 ctcggcgaga atcccttcgc aaatcaattg ggcccgcgtc aggtttttga cccgcagggc
     1201 gttctggtac gcattgaaca caagctgccc aatcgccgcc agcgacgtaa gcaaaatggc
     1261 cagcgcgaga aggacttcca acagcgtgaa cccatcccgc cgatggcgat cgggtttgtt
     1321 gctggtcgga agttttttct cacatgtgaa catgacaaca cttctgccga tcgtgggccc
     1381 gaagctcatc ccaacccagc gaatggcaac cacactatga tcccgccacc gacggggcgg
     1441 cgggcagttc cgtcatcccg gccgattcag agtcatacaa ctcaccgacc aggacggccc
     1501 cggtcaagcc acgcaaagaa atgtcaatcc gtcggccaaa ctcattctcc aacgtgatca
     1561 gggccgaact cgttgttcca tctgggaaaa acaggatcga cgacccgtcc gatggtgggc
     1621 tcgagagcgt tggatcctcc gtgatagccg ccgttagctc cccgccttcc acctgaacat
     1681 ccacgaatgt gattttttcc ggaagctctt tccgcgaacc accacccggg atttgcatca
     1741 gcggcacatc ggtcatgtct tgagtcccca tggaagggtc ataaacatcc gtcccgggca
     1801 ccgaggcatc gtaggggaca aggacgtaac cgttgccgcc caattcacac tgaaagtaat
     1861 acgtctgacc attggccatc gcacgatttc tggcccggat ccattcggcc cggatttgat
     1921 ctgcggccag ttgcaggcgt cgtgccgcca tgggacgcat cagggcattg aaagctacgc
     1981 ccgacaggat caccaccagg acgaggacaa tgaccacctc gaggagggta aaggcaagac
     2041 gggggacatc gccgcaggcc cgcttagacc gaccaggcgg gacaacggtc cattgcacct
     2101 tccgcgcggc ataggtccgc ccgaacacca aactcatcga gacttgtccc ctccgctccc
     2161 aaaacgcgtc ttaacaccat gtggcttact gaccccaatt ggtgatgtcg tcctcggtgc
     2221 cgtcaacacc atccggcccc attgaccaaa tgtccggctt atcggtgtta tattgcccag
     2281 ggtagaggta ctggtattcc cgaccccacg gatcaagggg aacggcttgg gcatccaagt
     2341 agggaccgtt ccatttggtg gggtcagcga ggtccgccgg cggcgtaatg agggcatcaa
     2401 gtgacggcgg gtactcattg aagtctaggt aatacgcatg caagggctgc tcgaacgcct
     2461 tgatttgagt cctcgccgcg ttgatgtacg cccttcgctg caccgcgatc aagttcgtta
     2521 cggccagcga ggctatgatg aggaggatcg taaggacgat caacacctca atcagcgtca
     2581 aacctttgcg agtggtccgc gacagtttct ttcgcacaaa gccttttgtc tgtctcatct
     2641 tcccatcctt ttgtcgttgc catcccattc gtgtggtcgg tgccaatcac ccaatcggcg
     2701 gggatcgccg ccgtggtctg ggcgtgacac ttccctatcg tataaacaat ccaaaaccgc
     2761 ttgaggtacc attttccata caacgatttt ttcagaggga atttggcagc aagtccgctg
     2821 ccaaaaagcc tggcaggttt tgcgaataag agcctatccc agaatctcgc cccacccgtt
     2881 cggcggggaa ggtcgggggt aaggatggct cgaatcgctg ctggtcttct caagcgattc
     2941 cgacgttcgc ttgccctcac tccaaccctc tcccgggggg agagggagct ttttcggata
     3001 gactctaaaa cgactggcct gatttacact cctttaggtg ggacgtttgt atcgctcggg
     3061 tggttagtca cccactgcgg gtcaacaccg gggtgcggcg agttaacctc attttacccg
     3121 acagcaccgc tcatcttaaa caccggtaac agcagcgcca cgaccacgac cagcaccgcc
     3181 atggccacca gcagcaacag gatgggctcc aacaagcgaa cccccacatc cagttgtcgc
     3241 cacgtccgcc gctccaatga atcggcaatt tgcaagagga cggtctccaa cgtattcgcc
     3301 tcctcggcca cggcgatcat ttccaccact aaggggggaa agagtttgca cgccgccagc
     3361 ggttccgcca ggcgctgacc ttgtgaaatg tttttggtgg ctttgtgaac cgcctctccc
     3421 agcaggacgt ttcccgtggc ttcactggca atttctaacg cccgcaggat gggcacacca
     3481 tttttcaaca gggtccccaa aactcggcaa aatcgggcta ccgcaaagtt gagcacaatc
     3541 ccacccagaa tcgggacctt cagaacataa cgatcccaca accgccgccc cgtctcgctc
     3601 ctcagccaag ccctcacact gaagaagccc aagatgatca cgacaacgac ccagatgccc
     3661 catgcctgaa ggaattggct gaaggccagc agcccttctg tcaggcccgg caattcattc
     3721 cgttcccgca ggttctggaa catcccctca aactggggca caagaaagat caaaaggccg
     3781 ctgaccagca atgacccgat cacgatgagg aacatcggat aggccatagc ccccttaacc
     3841 cgttttcgca ggtcttccgt cgcctcggta aactcggcca cacggtagag ggcctcttcg
     3901 aggaacccac cttcgctccc cgcacggacc atactgctgg ccatctcgct gaagacaccc
     3961 ggaaaccggg ccatggcatc accaaggccc ataccatcct caacgcgccg ggcaacttcc
     4021 ccgagaacct ccttgagtgt cgggtgaacc gactgacttt tcacaatgtt caaggcacgg
     4081 agaaggggca cgccgcttcg cagaaggtcg gccagttgac cataaacgac agccattgcc
     4141 tgggcaggaa cagatcgtcg gccaaagctg aaaagaggcc gcgcgggggc ctcgcttcgc
     4201 acctccaacg ggtaaagctc ccgactggcg atggccgtca gggcctcccg ttttgtggcg
     4261 gctgcgattg tccccgtcac ccgctggcca ttcgcatcaa cggcgacgta actgaactca
     4321 ggcattttgg gcggccgcta tgatcatctt tgaccgggtt ctatctcgcg gtgactggta
     4381 ccgaggggag ggccatgggt taatgctctt cggcccgcgt cacccgctcg atctcttcaa
     4441 ttgtagttcg cccactcaat actttccgcc atgcgtcctg ccgcagggtg atcatcccct
     4501 cttcgatcgc tgtctgccgg atcacccaac tgctagcccg gtcgtgagcc agttcccgaa
     4561 tcctttcgct ggtgacaaga agctcgaaaa tacccacccg gcctcggtaa cccacgtgcc
     4621 ggcacgcccg acatcccacc gcccgatata acgtgacgcc ctgctcgagg atttcccgcg
     4681 ggaagtcctt gggcaattct tccggtcgtg gccgataggg ttccttgcat tcggggcaaa
     4741 gtgtcctgac caatcgttgg gccatgacgg cctcaaccgt gctggagacc aaaaacggct
     4801 ccacgcccat atccaccaaa cgggtatatg ccccggcagc atcgttggtg tgcaaggtgc
     4861 taaacaccag gtggccggtc aacgacgcct gaatcgcgtt tgcggccgtc tccaggtcac
     4921 ggatttcgcc cacgaggatg acatccgggt cgtgacgcaa gatgctgcga agcgaatggg
     4981 caaaggtcag tccgatcttg ggatggacct gaatctggtt gatcccttcc aactggtact
     5041 cgacagggtc ttccgtggtg atgatcttga tcgattcatc cctaatttca agcaaggcac
     5101 tgtaaagggt cgtggtctta c
//
LOCUS       DAOPTR010000123         5092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_13276_length_5092_cov_3.519952, whole genome shotgun sequence.
ACCESSION   DAOPTR010000123 DAOPTR010000000
VERSION     DAOPTR010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13276_length_5092_cov_3.519952"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PKI05_03595"
     CDS             complement(<1..445)
                     /locus_tag="PKI05_03595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA51313.1"
                     /translation="MPPLVQVLHSLECGGAERLALRLAQGLAPSEVTTFICLDSRGPL
                     ATEAEANGFEVHVLDRRPGVDWACRKKLLDLLRRIRPSVILAHQYTPFFYSSLARGFA
                     RQPPIIFVEHGRHFPDPRKRRRVLVNRLLFRGSDVVIAVAESVKRA"
     gene            complement(488..1015)
                     /locus_tag="PKI05_03600"
     CDS             complement(488..1015)
                     /locus_tag="PKI05_03600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HOA51314.1"
                     /translation="MGVLAKPDYYFSSVTKIDIPFLRKENIACLLLDVDCTIADYRRG
                     ELDHVVAEWLRQLQEAGIQTCLVSNGGRRRIKRLAGHLGMPFVARSLKPFPWGCRRAI
                     KLLGVSPEQAAMVGDQLFADVLAGRWAGLRTILVQPRSGIKEPWVTRWKRPIERWLLG
                     RWGLLPAQDGTKENE"
     gene            complement(1053..2873)
                     /locus_tag="PKI05_03605"
     CDS             complement(1053..2873)
                     /locus_tag="PKI05_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51315.1"
                     /translation="MHQASSGGDHWNSFNHVNARGQVTLPFRGYRAISPEGTTSGDRA
                     DPLACLQTAAYSCGFWMPDFWQEFPKRIDVGPRSSTLAFLAENPEDVHELQGGEQKTY
                     VAWIVLSDGSDDGRHAVTTQLMALCRRVTVRSALQQALVELPSDLPPPELWPGTALAQ
                     FAEEAGFGPKGLLAGREEIDEYGWRHFGDVYASHEKAFYEGRLPFVSHYNNQFDLLCG
                     LILCRLVSNDPRWEELIHPLARHVIDIDIYHTLRDRSTFNGGMFWMTDHYVTAATATH
                     RAFSRENAQRPGYGGGPSNEQNYTSGLTLYYLLWGDEDARDAVLSLANWVISMEDGRQ
                     TPFFIADDGPTGLATATSSPDYHGPGRGSANSVNALLDAWLISGNAVYRSWAEKLCRR
                     VIHPKDDPAAFGLLHAETRWSYTMFLEVLCKFLRIKCLFGEHDHTFEYLRRSLICYCD
                     WMVHHERPYLDHPEELEYPTEAWAAQEFRKATVLFLAAKFAEPERAARYRQKAIWFAE
                     KAWEELNRFESRINPRTAAVILNQAIWHLADAVSEGCACCLRLKDESLSLPPREIFIP
                     QRARVRRMLTSRRDWPRIAVRLANPYNWFRLIQILWQWRN"
     gene            complement(2932..3717)
                     /locus_tag="PKI05_03610"
     CDS             complement(2932..3717)
                     /locus_tag="PKI05_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51316.1"
                     /translation="MANSSKTLSIPIDLGTWGTPVDLPNVGVPLRFGVPLPRGKLSYP
                     CRGQLVSPSGAVFPTQIAPVATWPDGSARWVRIDTVIHACLTSQWRETWQLDFESLDE
                     PTGQGMPDRIPLRLGYSVVPTSSRDTVFAFVDRASNEPLAVAHVFMKTKAGSVRLGQC
                     RHLRWHVQGPICYEATAEGTFPGVRGLLWRVTLRVYPTLNAIHIKAMLQYPACSPPWW
                     AVGLRRSRINLIAGVRSSPGVARSAQAPRLAGQAIRGVPYRGR"
     gene            complement(3729..4361)
                     /locus_tag="PKI05_03615"
     CDS             complement(3729..4361)
                     /locus_tag="PKI05_03615"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOA51317.1"
                     /translation="MSPRLIIKRCVQVLVLSLGVLFWVLYRLEALIWGKRPAFACWSQ
                     CLSLLPGRCGCLVRWSFYFLAARRCSWDVTIEFGTIFSSPNIEIGRHVYIGAYCVLGD
                     VQIGNGVRIASGVSIPSGSRQHWVQVVGQGGDAGGPESPNQYDRVAIGERTWIGERAV
                     ILANVGTRAIVGAGSVVTSPVEDDSVVAGVPARVIRRLSPASSEIGVETS"
     gene            complement(4470..>5092)
                     /locus_tag="PKI05_03620"
     CDS             complement(4470..>5092)
                     /locus_tag="PKI05_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51318.1"
                     /translation="AGFVHTHTVYFHDVTQTFCPPLDPEAKPEEYVGWCFEKLLFGNA
                     ICNSSVLVRRELFAQVGLCDPIIEGNTVQDYDLWLRLSQVTPFAYVDEPLTVFRLHSH
                     QGTSNHRMMLREQIKVLQRRLPGLPSALRHSVARRLARLYDLLGSYELDYHDHRAARW
                     AFRQSFRWEPHLRSLCLWFVTFLTPRDVAKLRSLYARWKGFRARKS"
BASE COUNT         1191 a         1527 c         1388 g          986 t
ORIGIN      
        1 cggcgcgttt gaccgactcg gcaacggcga tgacgacatc actaccgcgg aagagaaggc
       61 ggttgacgag cacacggcgg cgtttccgtg gatcggggaa atgcctccca tgttcgacga
      121 agatgatcgg tggctgacga gcaaaccctc gggccagact gctgtagaaa aacggcgtgt
      181 actggtgggc cagaattaca ctgggtcgta tccggcgaag gagatcgagg agcttctttc
      241 ggcaagccca atccacgccc ggccggcgat cgaggacgtg cacctcaaac ccgttcgcct
      301 cggcctccgt ggcaagtgga ccacgactgt cgaggcaaat aaaggtcgtt acttcactgg
      361 gggcaagtcc ttgtgccagc cgaagggcga gccgttcggc cccaccacac tccaaactgt
      421 gaaggacctg aaccaggggc ggcataaagg ggtaattcgt ctcgcccggc gagaatcgga
      481 cggaaggtta ctcgttttcc ttcgtcccgt cttgggcggg aagcaaaccc catcggccaa
      541 gcagccatcg ctcgatgggg cgtttccagc gggtcaccca gggttctttg atcccactgc
      601 gaggctggac aaggatggtt ctcaacccgg cccatcgccc ggccaagacg tcagcaaaca
      661 attggtcacc caccatggcg gcttgctccg gcgaaactcc aagaagcttg atggcccggc
      721 ggcatcccca gggaaagggt ttcagtgagc gtgcgacgaa tggcattccc aaatgaccgg
      781 ccagccgttt gatgcgacgc ctccccccat tcgacacaag gcaggtttga atgcctgcct
      841 cttgaagttg gcgaagccac tcggccacca cgtggtccag ctcgccccga cggtaatcgg
      901 caatggtgca gtccacatcc aaaagaaggc aggcgatatt ttctttccgc aagaaaggga
      961 tgtcaatctt cgttaccgac gagaaatagt agtccggctt tgccaagaca cccatggatg
     1021 ctcctcaacc tgcactgtcc caactgtccg cttcagttgc gccattgcca gaggatttgg
     1081 atcaagcgaa accaattata cgggttggca agccgaaccg cgatccgtgg ccaatcccgt
     1141 cgggaggtga gcatccgtcg tacacgggcc ctctggggga taaagatttc gcgtggcgga
     1201 agactcagtg actcgtcctt caaacgtagg caacacgcac atccctcgct cacggcatcc
     1261 gccagatgcc agatggcctg attcaggatg acggctgccg tccgcggatt gatccgagac
     1321 tcgaagcgat tcaattcctc ccaagctttt tccgcaaacc agatggcttt ctgacgatat
     1381 cgagcggccc tttctggctc ggcaaacttg gccgccaaaa agaggaccgt ggccttgcgg
     1441 aactcctggg ctgcccaggc ctccgtggga tactccaatt cttcgggatg gtccaggtat
     1501 ggccgttcgt gatggaccat ccaatcgcaa tagcagatca gcgaacgccg caggtattca
     1561 aacgtgtgat catgttcccc aaaaagacac ttgatccgca gaaatttaca cagcacttcg
     1621 agaaacatgg tgtacgacca ccgggtctca gcatgaagca ggccaaaggc agcgggatcg
     1681 tccttaggat ggatgacgcg tcggcaaagc ttttcagccc agcttcgata aaccgcgtta
     1741 cccgaaatta accatgcatc gagcagggca ttcacggaat tggcggagcc acgtcctgga
     1801 ccatgataat cgggcgaact tgtggccgtg gccagcccgg tcgggccatc gtccgcaatg
     1861 aaaaaaggtg tctgccgccc gtcctccatg gaaatgaccc aattggcaag agataggacc
     1921 gcatcccgag cgtcctcatc tccccagaga aggtaataga gcgtcaaacc gctcgtgtag
     1981 ttctgctcgt tgctgggccc accgccgtaa ccaggccgct gggcgttttc ccgcgaaaaa
     2041 gcgcgatgcg tggccgtggc cgccgtgacg taatgatcgg tcatccagaa catgccgcca
     2101 ttaaacgtgc ttcgatctcg caatgtgtgg tagatgtcaa tatcgataac atggcgtgcc
     2161 agcggatgaa tcagctcttc ccatcgcgga tcgttcgaca ccagtcggca cagaatcaac
     2221 ccgcataaaa gatcgaactg attgttgtaa tgcgagacaa acggcagcct cccctcatag
     2281 aaggcctttt cgtgcgaagc gtacacatct ccgaaatgcc gccagccgta ttcgtcgatc
     2341 tcttcccggc cggccagtag ccccttcggc ccgaagccag cctcttcggc aaattgggcc
     2401 aacgccgtgc ccggccaaag ctccggaggt ggtaagtcgg aagggagttc cacaagcgct
     2461 tgttgcaatg cagatcggac cgttactcga cgacacaaag ccattaactg cgtcgtcacg
     2521 gcatggcgac cgtcatcgct cccatcggac aagacgatcc aagccacgta cgtcttttgt
     2581 tcccctccct gaagctcatg cacatcctca ggattctccg caaggaaagc gagggtgctt
     2641 gatcgtgggc ccacatcaat ccgtttggga aactcctgcc aaaagtcggg catccagaac
     2701 ccgcaggaat aggccgctgt ttggaggcag gccaacgggt cggcgcggtc cccagatgtg
     2761 gtgccctccg gcgaaatagc gcgataacca cggaacggaa gcgtcacttg tccccgtgca
     2821 ttgacgtggt tgaagctatt ccagtgatcc cctccgctcg aggcttgatg cacttgccaa
     2881 atcaatgagt caccgtcacg agaaccggtt gagtttttcc ccggccacct gtcaccgtcc
     2941 acgatatgga acacctcgga tggcttggcc cgccaaacga ggtgcttgag cggatcgcgc
     3001 cactccaggc gaagaccgaa ctcccgcaat aagattgatc cgggatcgcc taagtcccac
     3061 agcccaccac ggtggcgagc acgccgggta ttgcagcatg gccttgatgt gaatcgcatt
     3121 cagcgtggga taaaccctca gcgtcactcg ccataacagt ccccttaccc ccggaaatgt
     3181 cccctccgct gtggcctcat aacaaatggg tccctgcacg tgccatcgga gatgacggca
     3241 ttgccctaac cgcacgcttc cagctttcgt tttcatgaac acgtgggcca ctgccagcgg
     3301 ctcattcgaa gcgcggtcca caaaggcaaa gacggtatcc ctggaagaag ttggtacgac
     3361 cgagtatcca agtcgaagtg gaatacggtc aggcatgccc tgcccggttg gttcatccaa
     3421 ggactcgaag tcgagctgcc aggtctcacg ccattggctt gtgaggcagg cgtgaatgac
     3481 cgtgtcaatt cgcacccagc gggcacttcc atcaggccaa gtcgctacgg gagcaatttg
     3541 agtgggaaat actgctccac tcggagaaac caattgacct cggcacggat agctcaactt
     3601 ccctcgcggc aacggcacac caaagcggag cggtactcca acgttaggca aatctacggg
     3661 agttccccat gtgccgagat caatcggtat cgacaaggtt tttgaactgt ttgccatgat
     3721 gctgcccttt aagacgtttc cacgccgatt tcgcttgacg ccggggataa tcgtcggatg
     3781 acccgggcag gtacacccgc cacaacggaa tcgtcttcca caggagaggt caccacgctc
     3841 ccagccccca cgatcgcccg cgttcccacg ttagccagaa tcacggcccg ctccccgatc
     3901 catgtccttt cgccaatggc cacccgatca tattgattcg gagattctgg tcccccggca
     3961 tcgccacctt gaccaacgac ttgcacccaa tgctgtcggc taccactggg gatggacacg
     4021 cccgatgcaa tccggacacc gttgccaatt tgaacatccc caagaacgca gtatgcgccg
     4081 atatagacgt gtcggccaat ctcaatattg ggtgatgaaa agatcgtacc gaattcgatg
     4141 gtcacgtccc aactgcaccg ccgggcggcg aggaaataaa aactccatcg caccaagcaa
     4201 ccacatcgtc cgggaaggag agaaagacac tgcgaccaac acgcgaaggc cgggcgtttt
     4261 ccccagatta gggcttccag acgataaagc acccagaaca acacgcccag ggagagaacc
     4321 agcacttgaa cacatcgctt gatgataagc cgcgggctca cagcttctct attcacccca
     4381 ttttctggag gatcacttga catcgaggat gccgatacct ctaacctaaa tgagcgtagg
     4441 tcgccgttct ttcccggaag attacttatt cacgattttc tcgctcgaaa acccttccaa
     4501 cgggcataga ggctccggag cttggcgacg tcacgaggtg tcagaaaggt tacgaaccac
     4561 aggcacaagg aacggagatg cggttcccaa cggaatgatt gccgaaaggc ccagcgggcc
     4621 gcgcggtggt cgtgataatc cagttcataa cttcccagga gatcataaag gcgggccagc
     4681 cgccgcgcca cggaatggcg cagtgccgat ggtaaacctg gcagtcgccg ctgaaggact
     4741 ttgatctgtt cgcggagcat cattcggtgg ttgctcgtcc cctgatgcga atggagacga
     4801 aagaccgtca acggttcatc aacataggca aacggcgtga cctgggacaa tcgcaaccac
     4861 agatcgtagt cttgaaccgt gttgccctcg atgatgggat cacacagccc tacctgcgcg
     4921 aaaagttccc gccgtacgag gacactggag ttgcaaatcg cattgccaaa caggagcttt
     4981 tcgaaacacc agccgacgta ctcctccggt ttggcctccg gatcaagggg tgggcagaac
     5041 gtctgtgtca catcgtggaa gtacacggtg tgggtgtgaa caaatccagc gt
//
LOCUS       DAOPTR010000124         5071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_13360_length_5071_cov_3.542464, whole genome shotgun sequence.
ACCESSION   DAOPTR010000124 DAOPTR010000000
VERSION     DAOPTR010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13360_length_5071_cov_3.542464"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(271..1557)
                     /locus_tag="PKI05_03625"
     CDS             complement(271..1557)
                     /locus_tag="PKI05_03625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOA51319.1"
                     /translation="MSRPHEPKTNHQGRMCLVVVFLAALAVRVVAAVAWEWHFGPRFV
                     FGDSESYWHLARCLAHCQPYRFGDFGTVFRTPGYPALLAPLFWFFDSPPTLAARIEGS
                     LLGAIGALVTAIWAGNLFGSRARWLAGLIMAFHPEMVLSSILILSEGGFLPVWLVHLL
                     VLSQALSRTHAAKTAWKEWFLAGCLVGLATLIRPAAILWLPFCATVTITFFTRQRLQL
                     VILWAVCLLAFGLTMSLWWVRNYRVTGRWILTTTQAGPTLYDGLHPGATGASDMSFVP
                     NKFRELQQSRPDARPIDLELEFDRRLSAEAIRWAWSHPRQVVSLAIVKFRRLWNPFPN
                     DPGFNVWPINLIVAGCLLGVLVLGLAGCLKFKSIGWPVAVCWLPAVYVTLLHVIFVAS
                     IRYRWPAIPGLIVLGAGFLAETIQGIRVRDVKMVDT"
     gene            2092..3567
                     /locus_tag="PKI05_03630"
     CDS             2092..3567
                     /locus_tag="PKI05_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51320.1"
                     /translation="MAAQLPDYVTSTKPVPKDKRTPWYASTAPTYAGIFLWFVFWHQV
                     PLLKELTPGSGIPLTAGGALSQGLGIALLALIVSALICHYLFYLVPGKLGVQTGLPLY
                     VVGTSTYGVLGGFIMPGFLMGVLQFGWLAVNAFFTTGLLCAPFGAGPGTLTFTVVGIA
                     FVILATLVGLKGIQYVGKIATYLPLLPLFILVVLTFASVGGLSKFDHKKLVGAPAAAS
                     ENAPAGGEQKAEATSAASAKQMPDLSVWGVFMAICAYVVGFFATAGAAGVDFGMGNRD
                     ENDVKWGGLVGIAGAIILTGGLALIIAAGTVGAGLAGQPAVLQPTKLMENLAPVLGEK
                     APAVFWYLLAIAAFPPACFSSFIAANSFKTTLPKVNPFVSCGIGALASIALVLSGYAG
                     NAANVFTVIGASFGPVCGAMAADYILAGNRWAGPRAGFNPAGWVSWVAGFIVGAVDLL
                     LGTSYVPCPPVAAFIVGFVLYAILALAGLESELLPMPKEGE"
     gene            3813..4550
                     /locus_tag="PKI05_03635"
     CDS             3813..4550
                     /locus_tag="PKI05_03635"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HOA51321.1"
                     /translation="MNAAMDGKTSHPRLVLPRRWVRELDRRAVEEYHIPSIILMENAG
                     RGAADVLERLGINGPVVIVCGRGNNAGDGLVIARHLDLRGYKVKVVMLAPGESFTGDP
                     FVNYQIVRCAQLPMVILGEGCSEHEVLAHLNGADWIVDALLGTGAQGDPRPPFDVVIH
                     AINRAHDQGKKVLAVDLPSGLDCDTGQPSRATIRADHTVTFAAVKPGLIVDAARPFVG
                     QLHVADIGTPRRLIDEIAAEYERESNS"
     gene            4578..>5071
                     /locus_tag="PKI05_03640"
     CDS             4578..>5071
                     /locus_tag="PKI05_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51322.1"
                     /translation="MSSQPHHPPNGFGSPPLAVVPATGEGIRRGLWLLCKDLPEADRD
                     GHLRRLREHFRRSPPGTTGILQAQRGQEIVGAILWTVYPGRTAVISAPGLLENEPLTT
                     ARALFRGVVDNLANTQTRLIFANFSQDQGHFREPVEEAGFCYLARLLYLFSPVADLPN
                     SSEPP"
BASE COUNT         1139 a         1302 c         1478 g         1152 t
ORIGIN      
        1 gcggatcgga tgcgaatact caaactgggg aatagttcag agcaatgggc ttcaatagcc
       61 cgcttcagtt tcgaatcaag gctgcggtaa atggtaaatg cgtaacaaag acccgctaca
      121 agtgagatca acattcccca cttgcagaat tgccaaaccg gtcttgcggg tcttcgccgg
      181 tccttcatcc tagacgacca tccgtggcat tactgtgggt actccataac cttgcaggac
      241 attcaaaaca aaaccacgtt gcgaaccagc ctaagtgtcc accattttta catcgcgaac
      301 tcgtatcccc tggattgttt cggccaaaaa accggccccc agcacaatca gtcctggaat
      361 ggcgggccat cggtaacgga tggacgccac aaagatcacg tgaagcagtg tgacatatac
      421 cgccggtaac caacagacgg ccacaggcca accgatgctc ttgaatttta ggcaaccagc
      481 cagtcccaag accaaaactc cgagcaaaca acctgcgaca atcaggttaa ttggccagac
      541 gttaaatccc ggatcgttag gaaacggatt ccacagtcgc cgaaacttga caatagccaa
      601 gctcaccacc tgccgaggat gtgaccaagc ccagcggatg gcctcggcag agaggcgtct
      661 gtcaaattca agctcaaggt cgatcgggcg agcgtccggc ctcgactgct gcaattcgcg
      721 gaatttgttc ggcacaaagc tcatatcact ggccccggta gcaccgggat gcagaccatc
      781 gtaaagggtt gggcctgcct gggttgtggt gagaatccat cgtccggtta cccggtagtt
      841 ccgaacccac cataaagaca tggtcaatcc aaaggccaaa agacagaccg cccaaagtat
      901 gaccagttgc aaacgctgcc ttgtgaagaa agttatcgtt acggtggcgc aaaacggaag
      961 ccacaagata gctgccggac gaatgagtgt ggctaagcct acgaggcagc ctgcaaggaa
     1021 ccactctttc catgccgtct tggctgcgtg ggttcgggaa agtgcctgag aaagtaccaa
     1081 taagtgaaca agccagacag gcaggaatcc tccttcactg aggatgagaa tactcgacag
     1141 gaccatttcg ggatggaatg ccatgatcaa gccggctagc catctggcac gactgccaaa
     1201 gaggttgccc gcccagatgg ccgtgacaag ggccccgata gcccccagta agcttccctc
     1261 aatgcgagct gccagggtcg gcggcgaatc aaaaaaccaa aacaggggcg ctaaaagggc
     1321 agggtagcca ggcgtccgaa aaacagtgcc aaaatctcca aaccgatatg gctggcaatg
     1381 agctaagcat cgggcgaggt gccagtagct ctcgctgtcg ccaaagacga agcgaggacc
     1441 aaaatgccat tcccaggcca ctgcggcgac gacccgcacg gccagggcag ccaaaaacac
     1501 cacgaccagg cacattcgtc cctggtgatt cgtcttcggc tcatgcggcc gggacatgct
     1561 caaaatgaaa cgcttccttt ctggggttta gcctaaaact ccgacgacgg cgaaaaagcg
     1621 gcggttactg tcagccttgc aacagacgtc ttaacgtcgc atcaagtcat actagtgagg
     1681 cttcagaatg ggaatggtac acgtattcgg aaggttacgt caatgcgaaa atgggaaaac
     1741 gaaagtggtg ggagaatatg agcaacctgt gatggtttgg ttctgcagga aaggtgtggg
     1801 cagccggttt ggtggaaacg agacgagaaa ataaacagtc acaaatgacg cacttctcct
     1861 tcctaatcca acgcctgcgc cgcatgctca ggtcgtgcgc tgcgacatgt gacagaaatc
     1921 agccttttcc gaagtaattc ctcacaaatt ggttatggac aatgtcaaca gttcggttat
     1981 actcaatgcc aattgggacg gtgatgatgc tactgcttgc agtgatgtga ggatgggtgt
     2041 ttctttggtc tcaggagagg tcctgttttg acaagcaaag gaggttactc aatggccgcg
     2101 caacttccgg attatgtcac cagcactaag ccggtgccca aagacaaacg cacgccgtgg
     2161 tacgccagca cagcgcccac atacgcaggg atttttctct ggttcgtgtt ttggcaccaa
     2221 gtcccgttgt tgaaggagct gactcctgga agtggtatcc ctttgacggc aggaggggcc
     2281 ctctcacagg gattggggat tgcgctcctg gcgttgatcg tatctgcctt gatctgtcac
     2341 tatctgtttt acctggtgcc cggcaaattg ggggttcaaa ctgggctacc gctctacgtg
     2401 gtggggacat ctacctatgg cgtcctgggc ggtttcatta tgccgggttt cctcatggga
     2461 gttctccaat ttggctggtt ggcagtgaac gcctttttca caactggctt actttgtgct
     2521 ccctttggcg ccggcccggg tacgctcaca tttaccgtag tggggatagc cttcgtgatt
     2581 ttagcgacat tggttggcct aaaaggcatt cagtacgtgg gtaagatagc gacctatctc
     2641 ccattgctgc cgttgtttat tttggtggtc ttgacattcg cctccgtggg cgggttgtcc
     2701 aagttcgacc acaagaagct ggttggggcc ccagcggcgg caagcgaaaa tgcaccggca
     2761 ggtggggagc aaaaggcaga ggcgacctcc gcagcgtcgg ccaagcagat gccggacctt
     2821 tcggtgtggg gcgtgttcat ggcaatttgc gcttatgtgg taggcttctt tgcgacggct
     2881 ggagcggctg gggtcgattt cggcatgggc aaccgcgatg agaatgacgt caaatggggt
     2941 ggtttggtcg gaattgccgg tgccatcatc ctcaccggcg gtctcgcgct gatcatcgcg
     3001 gcggggaccg tcggggctgg cctggccggt caacctgccg tgcttcagcc gactaaattg
     3061 atggagaacc ttgccccggt cttgggagag aaggcgccgg cggttttctg gtatctcttg
     3121 gccatcgcgg cttttccccc ggcttgtttc tccagtttca ttgcggctaa cagctttaag
     3181 accacattgc cgaaggttaa tccattcgtg tcatgcggca tcggcgcgct cgcctcgatt
     3241 gccttggtgc tttcaggata cgcaggaaat gccgccaacg tgttcaccgt gatcggggcc
     3301 tcctttgggc ccgtatgcgg ggccatggcg gcagactaca tcctggccgg taatcgctgg
     3361 gctgggccgc gagcgggctt caatcccgcc ggttgggtct cctgggtagc gggcttcatt
     3421 gtaggtgccg tggacctgtt gttgggcacg tcctatgtgc cgtgtccgcc ggtggcggcc
     3481 tttattgtcg gttttgtgct ctacgccatt cttgctctgg caggcctgga gagcgaattg
     3541 ctcccgatgc caaaagaggg tgaatgaaac gtttgaaagt ggtcttttga gaccaacata
     3601 ctggacggaa tgatgtcggg gggccttccc aaaaaggccc cttttcattt gatctttttt
     3661 gtcacacact gggtgtagat tggtaggata gaaaatcgtc ggaattccat tggcacgatg
     3721 tttccactac gggaacgagc gttgcgtgag ataaagagaa ttatcgcgca ggcgatcggc
     3781 aagctgagat catttggaaa gtgattttgg gtgtgaatgc ggcgatggat ggtaagacct
     3841 cgcatcctag gctcgtactt ccgcgacgtt gggtccggga attggaccga cgtgcggttg
     3901 aagaatatca cattcccagc attattctga tggaaaatgc cggacgtggt gcggccgatg
     3961 tgttggagcg gttggggatc aacggccccg tcgtgatcgt ttgtggaaga gggaacaacg
     4021 ctggcgatgg gttggtcatt gcccgccacc tcgacctgcg cggttacaaa gtcaaagtcg
     4081 tgatgcttgc cccgggtgag tcattcaccg gcgacccatt cgtcaattat caaattgtcc
     4141 ggtgtgccca gttgccaatg gtcatcttgg gagagggctg ctcggaacat gaggttttgg
     4201 ctcacctgaa cggtgccgat tggattgtcg atgcgctttt gggaacgggc gcgcaaggag
     4261 acccgagacc gccctttgat gttgtcatcc atgctatcaa ccgtgcccat gatcaaggga
     4321 aaaaggtcct ggcggttgat ttgccgagtg ggttagactg cgatacgggg caaccgtctc
     4381 gggcaacgat tcgagcagat cataccgtga cattcgcagc agtaaaaccc ggtttgatcg
     4441 tggatgcagc gagaccgttt gttggtcagc tccatgttgc cgatattgga acaccgcgcc
     4501 gattgatcga cgaaattgcg gctgaatacg aacgcgaatc gaacagttag cacttggttg
     4561 ggtagggcca gaagagtatg tcgtcgcaac cgcatcatcc cccaaatggc ttcggttctc
     4621 caccccttgc agttgttcct gctacggggg agggaattcg acgaggactg tggttactgt
     4681 gcaaagacct gcccgaggcg gatcgggatg gacatttgcg gcgattgcgg gaacactttc
     4741 ggcggtcgcc tcctggcacg acggggattc ttcaggcaca aagggggcaa gagatcgttg
     4801 gggctatctt gtggacagtt tatccggggc gaactgcggt catttccgcg ccgggattgc
     4861 ttgagaatga accactgacc acagcccggg cgctgtttcg gggagttgtt gataacctgg
     4921 ccaacacgca aacgcgattg atttttgcca atttttccca agatcaggga cactttcgtg
     4981 agccggttga ggaggcaggc ttttgctatt tagcccggct gctttatctt ttctcccctg
     5041 tcgcggatct cccaaatagt tcagaaccgc c
//
LOCUS       DAOPTR010000125         5065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_13390_length_5065_cov_3.394810, whole genome shotgun sequence.
ACCESSION   DAOPTR010000125 DAOPTR010000000
VERSION     DAOPTR010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13390_length_5065_cov_3.394810"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PKI05_03645"
     CDS             <1..335
                     /locus_tag="PKI05_03645"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOA51323.1"
                     /translation="HDVAQILQGLRMILKQWEDVLARHHCQKIDALQKPFDPNVHHAI
                     SRQATNDVPPNTVICVAQDGFILHDRVVRPSQVVVSMALPTAGSEGSSEAVPSSPGDE
                     SPGDHPPS"
     gene            420..704
                     /locus_tag="PKI05_03650"
     CDS             420..704
                     /locus_tag="PKI05_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOA51324.1"
                     /translation="MPTYEYLCEACEHRFEEFQSITADPLTKCPACGKKKLRRLIGTG
                     GAILFKGSGFYCTDYRSESYRKAAARDNGNGSSSSKSSETSSSTKSNGKD"
     gene            899..1915
                     /locus_tag="PKI05_03655"
     CDS             899..1915
                     /locus_tag="PKI05_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51325.1"
                     /translation="MAVPQDRRQFLQQAGLIAAAGAAVSLEEKILLAGVQQEKTGEAE
                     QSASNLAAEMPHGKVRNISISRLVIGGNLIGGFAHSRDLVYVSRLLKAYNTEEKVFET
                     LSLAERHGINTIQCNPACLPMVEKYNKERGGKLQAIVCITPDADKDTVRREIDEVLSH
                     GATMLYTHGGRSEPPVMSNRLDVLANTIECIKAAGVPAGIGSHSLTVTIECEKNGIGA
                     EFYVKTLHSDQYWSATPKELREDFCWQKPRRDEPFAFHDNMWCLNPEETIEFMKSVSK
                     PWFAFKILAAGAIHPRIGFPYALRNGADFVIVGMFDFQIAEDTAIFLESFKKLGRRER
                     PWCA"
     gene            complement(2109..3650)
                     /locus_tag="PKI05_03660"
     CDS             complement(2109..3650)
                     /locus_tag="PKI05_03660"
                     /inference="COORDINATES: protein motif:HMM:NF015764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOA51326.1"
                     /translation="MSEKMLLRVRLSAMMFLQFFVWACWMVPIGTYGPEALGLTGAHM
                     GWIFSTTAIAAIISPLFVGYVADRLFATEKVLCALHLVGGICLFLAGLQGRFEFLFLF
                     LMISTLAFMPTLALANSLTFRNLDDPATFPRIAMFGTIGWIISGWIVGFLLGERSPAF
                     FYLGGVIEVLLGLYALTLPHTPPQAKESKVSDVLGLGAVKLLKDPEFLLFTVCAFLIG
                     IPAVYYFVSCNLYLTQIGAPAPTALMTLGQVCEIVAMAAMPFFIAKIGLSRILAIGML
                     VWALRYALFATLSFPLALLGILVHGFCYVFVYVGSYIFVDKKAPRELRASAQSFIAFL
                     MLGVAWLIGSNLAGRTNDAYPPPIRGILAGVIGKDLPSDFTPPAWPTLAKAFDKNGDD
                     VIDSSEIAGVPSDDQNARAWMIMLREATPKVSAKTYGHVKAAASGRDIYLDREDYLML
                     TRNKWRDIWLWPAIICLVVFAIFTLGMPKREEDQAGEAAQEPSPRPKGRLRKVPNPQR
                     RLSNR"
     gene            complement(3778..>5065)
                     /locus_tag="PKI05_03665"
     CDS             complement(3778..>5065)
                     /locus_tag="PKI05_03665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HOA51327.1"
                     /translation="YSLYKTDESMQAEVIANGDEITSGRILDTNSQWLSIRLEDLGIR
                     VMYHTCVGDDLQAMVAVFRQAIERSDVVVTTGGLGPTADDLTREALANVTGRQLIELP
                     EALEHVRRFFAQRQRPMPERNRIQAAFPVGSEMIRNPNGTAPGIAMTIPREDRSPCHF
                     FCLPGIPSEVYEMWPEVEERLRKIGAGKEYILHRDIKCFGAGESQVESMLPDLIRRGN
                     DPLVGINASKNTIILRISTKGPNRQACEEKILPTVEIIQQCLGDLIFGYDEEELQDVV
                     LARLSELGKTLAIVETDTSGMVADWLYQSKAQSQGKPFRGGIVLATEDGVHRIVGSPS
                     ASSGQPVPSDRQGLAVLLATHVRKLFGADIGLAVSSLKPTELSRVVSFAVGVVSGETS
                     FQQEIRFGSHPDFVRLTMGKHMLNTLRLWLKGKLTS"
BASE COUNT         1196 a         1396 c         1394 g         1079 t
ORIGIN      
        1 gccatgatgt ggcgcaaatc ctgcaaggat tgcggatgat cctgaaacag tgggaggacg
       61 tcctggcgcg gcatcattgt caaaaaattg atgccctgca gaaaccattt gatcctaacg
      121 ttcaccatgc tatctctcgt caagcgacga acgacgtccc gcccaatacc gtcatctgtg
      181 ttgcccagga tggatttatc cttcacgatc gggtcgttcg cccaagtcaa gtcgttgttt
      241 ccatggctct gcccacggcg ggcagcgaag gaagcagtga ggctgtgccg tctagtccgg
      301 gcgacgaatc tcccggtgat catccgccaa gctaaggctt caaactttgg cgccaactgg
      361 gcatgttgtg aaattctgac cacggatact cactcatttt ttagaataag gaggtcttta
      421 tgccgacata cgaatacctt tgtgaggcgt gcgaacatcg ttttgaagag ttccaatcga
      481 ttacggcaga tcctctcacc aagtgtcccg cttgcggaaa aaagaagctt cgccgcctga
      541 tcggcaccgg tggggccatt cttttcaaag gttccggttt ctattgcacc gattatcgga
      601 gcgagtcgta ccgcaaggca gccgccaggg ataacggcaa cggctcaagt tccagcaaat
      661 cttcggagac gtcttccagc accaagagca acggaaagga ttgacgccac gagtccccgc
      721 attcgcgatg ataaaaactg agtagggctc tgcgcgcttt ttgccgccaa gggtgtgaca
      781 gggggcacgc cgtttggtaa aatcacgcta gcacggccaa tgagagagtc gcttggccga
      841 tggtgccggt gttgaaagat gtatgtccat taagtcgcgg atgcggggag gaccgaaaat
      901 ggctgtccca caagatcggc ggcagttcct gcaacaggcg ggtcttatcg cggcggccgg
      961 tgcggccgtc agtctagagg aaaaaatact tctggcaggg gtccagcaag agaagaccgg
     1021 ggaagcggag cagtccgcct caaacctggc ggcggagatg cctcacggca aggtccgcaa
     1081 tatttcgatt agccgtctcg ttatcggcgg aaacctgatc ggcgggttcg ctcacagccg
     1141 ggatttggtg tatgtatccc ggctcctcaa agcatataac acagaagaaa aggtttttga
     1201 gacactatca ctggcagaga ggcacgggat caacacgatt cagtgcaatc cggcgtgtct
     1261 gccgatggtg gaaaaataca acaaggaacg cggcggcaaa ctccaggcca ttgtttgtat
     1321 cacccccgat gcggacaagg acaccgtgcg tcgggaaatt gatgaggtcc tcagtcacgg
     1381 ggcaacgatg ctctatacgc atggcggacg gagcgaacct cccgtcatgt ccaatcggtt
     1441 ggatgtgcta gccaatacga tcgagtgcat caaagcggcg ggtgtgccgg caggcattgg
     1501 cagtcattcc ctgaccgtca ccatcgaatg tgagaaaaat ggcatcggtg ccgagttcta
     1561 cgttaagacg ctccacagcg accaatactg gtcggccacg ccaaaagaac ttcgcgaaga
     1621 tttttgctgg cagaaaccga ggcgagatga accgttcgcg ttccatgata acatgtggtg
     1681 tctcaatccc gaggaaacaa tcgagttcat gaagtccgtt tccaaaccgt ggtttgcttt
     1741 caagattctg gcagccggtg cgatccaccc ccggatcggc ttcccgtacg ccctgcgtaa
     1801 tggggccgat tttgtgatcg tggggatgtt cgattttcag atcgccgagg atacggccat
     1861 ctttttggaa tcgttcaaga aactgggccg acgcgaacga ccgtggtgcg catgacgccc
     1921 aggcgaggtc ttctggttgc tgatttcatg gacagcattc aaagaataag gccagcctga
     1981 agatggctgg cctttctgtt atcacagtga actggagctt acccggaact taccccggac
     2041 ctgcggtcat gggctattga acggcgtatc ggtggcttcc ggaccttttg gcggcagtgg
     2101 cggcggcgtt accggttgct tagccggcgc tgcgggttcg gcactttcct gagccgtccc
     2161 ttcggcctcg gggacggttc ctgcgctgcc tcacctgcct gatcttcttc tctcttgggc
     2221 attcccaagg tgaaaatggc aaagacaacg aggcagataa tcgccggcca caaccagatg
     2281 tctcgccact tgttgcgggt cagcatgagg taatcctcgc gatcaagata gatatcccta
     2341 ccactcgcgg ccgccttgac atggccatac gtcttggcac tgaccttagg cgtcgcttcc
     2401 ctgagcatga tcatccaggc acgtgcgttt tggtcgtcgg aaggtacacc cgcgatttca
     2461 gacgagtcaa tgacgtcgtc accattcttg tcgaaggcct ttgccagagt gggccaggca
     2521 ggcggtgtga agtcggaagg cagatccttt ccgatcaccc ctgcgagaat gccacgaatg
     2581 ggtggcggat aggcgtcatt cgtgcgtccc gccaggttgg aaccgatcaa ccatgccacc
     2641 cccaacatga ggaaggcgat gaagctttga gcactcgccc ggagctcacg cggggccttc
     2701 ttgtccacga agatatagga gccgacgtaa acgaatacgt aacagaatcc gtggaccaaa
     2761 atccctagca gggccaacgg gaaactcagg gtagcaaaca acgcatatcg caacgcccaa
     2821 acgagcatgc cgatggccaa aatccgcgac aagccgattt tagcaatgaa gaacggcatg
     2881 gcggccatgg caacaatttc gcagacctgc cccagcgtca tcaaggccgt cggggccggt
     2941 gctccaattt gagttaggta caaattgcaa ctgacgaaat agtacacggc gggaatgccg
     3001 attaagaagg cacacaccgt aaagagcagg aactcaggat ctttcagcag cttaacggcc
     3061 ccgagcccca acacatcgct taccttgctt tccttagcct gaggtggcgt gtgcggcagt
     3121 gttaaggcgt aaagtcccaa aagtacctcg atgacgccgc caaggtagaa gaaagccggg
     3181 ctgcgctcac ctaagaggaa gccgacaatc cacccggaaa tgatccagcc gattgtgccg
     3241 aacatggcga tccgcggaaa cgtggccgga tcatccaagt tccgaaacgt gagggaattg
     3301 gcaagggcca atgtgggcat gaaggccaaa gtgctgatca tcaagaagag aaagagaaac
     3361 tcgaacctgc cttgcagacc ggcaaggaat aggcaaatcc cgccgaccag atgaagggcg
     3421 cacaagactt tctcggtggc aaacagtcga tcggcaacat atccgacaaa gagtggtgaa
     3481 atgatcgccg caatggccgt ggtggaaaag atccaaccca tatgggcgcc cgtcaatccc
     3541 agggcctcgg gtccatacgt tccgatgggc accatccagc acgcccacac gaagaactgg
     3601 aggaacatca ttgccgatag ccgaactctc agcagcattt tttcagacat aactcgttcc
     3661 tcacttttaa ggtccccgcc tcagatgcgt ctcttctggt ttcgaaccgt taaccgtgat
     3721 agagattcta gtctcaaaac accgccatac gcaacagggg cgttgagaaa cggacgatca
     3781 actcgttagt tttcctttca gccacaatcg gagggtattc aacatatgtt tgcccatcgt
     3841 caaacggaca aagtccgggt ggcttccaaa acgaatctct tgttgaaaag atgtttcccc
     3901 ggagacaaca cccaccgcaa atgaaacgac ccgcgacagc tctgtgggtt ttagcgaact
     3961 gacggccagg ccaatatccg cgccaaatag ctttcgcaca tgagtagcca acaagaccgc
     4021 taatccctgt cggtcgcttg gcaccggctg accgctgctt gccgagggag atcccacgat
     4081 ccgatgtacg ccgtcctcgg tagccaagac gataccacct cgaaatggct tgccttggga
     4141 ctgggccttc gactgataaa gccaatccgc caccatgccg ctggtgtctg tctccacgat
     4201 ggcgagggtt tttccgagtt cgctgagacg tgccaaaaca acatcttgta gttcttcctc
     4261 gtcgtaaccg aatattaaat cgcccaagca ttgctggatg atctcgaccg tggggaggat
     4321 tttctcttca catgcctggc gattcggacc cttggttgat atgcgaagga tgatcgtgtt
     4381 tttggaggca ttgattccca caagaggatc gttacctcgc cggatcaaat cggggagcat
     4441 cgactcgact tggctttcgc ccgcaccaaa gcacttgata tcgcggtgga ggatgtactc
     4501 tttccctgcc ccgattttgc gaagccgttc ttccacttcc ggccacattt cgtaaacctc
     4561 gctggggatc cccggcaagc agaagaaatg gcacggactt cggtcctctc gcggaatggt
     4621 catggcaatc cccggtgccg ttccgttggg gttccgaatc atctcgctac ctacgggaaa
     4681 agctgcttga atgcggttgc gctcgggcat ggggcgctgc cgctgggcga aaaagcgtcg
     4741 aacatgttcc agagcctctg gcaattcgat gagctggcgc cccgtgacat tggccagcgc
     4801 ctcacgcgtc agatcgtccg cggttggccc taatcctccc gttgtcacca ccacgtctga
     4861 tcgctcgatg gcttgacgaa agaccgccac catggcctgg agatcgtcac ctacgcacgt
     4921 gtgatacatg acacgaatgc ccaaatcttc caggcgaatg ctgagccatt ggctgttggt
     4981 atcgagaatc cgtccgctgg taatttcgtc accgtttgcg ataacttctg cctgcataga
     5041 ctcatccgtt ttgtagagtg agtac
//
LOCUS       DAOPTR010000126         5055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_13423_length_5055_cov_3.441800, whole genome shotgun sequence.
ACCESSION   DAOPTR010000126 DAOPTR010000000
VERSION     DAOPTR010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13423_length_5055_cov_3.441800"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3205)
                     /locus_tag="PKI05_03670"
     CDS             complement(<1..3205)
                     /locus_tag="PKI05_03670"
                     /inference="COORDINATES: protein motif:HMM:NF040127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HOA51328.1"
                     /translation="MLRIKTWLSLVALTLLVYAPSKYPTAQVTDAVELPRAGDGQWRL
                     NPNSSPEDIYRQVANGATAYLRSQVPLEWRLMWSAQRALAEAEALGKRVSQLEGVADR
                     MPVIGVGRACRMFEPWNRLAVNGEAVPHETDAAPSPRGGPDNEVVVGTGELLLEETDI
                     SLPDRGGLGLSFVRYYSSHVGYDGPLGPGWDHNCNQRIVAEGNPSKPTALIWYTGRRP
                     IRFEWKNGTWEPEPGAFYQLTLNDNQITIETPLRVRLTFTATSSSVGGGRWRIASIAS
                     RHDQWRANVIRFTYWPGSDRLQVVEDAYGNRLQFGYDPSGKLTGVVCGQLAVLYQYDE
                     LGRLTRVVLPRVATELTKTADVTWEYRYGTGLDGRTRLVAIIPPKGEPEKHYSYETDS
                     NAPAYGRVGGIVLRGAGDTPSVVEWRFETIHKEKTWTVIYKQPLPLPEERWTFPEIGR
                     WPGCYPLCREIPSQNATWSWVHNAAGQKLEARSPLGQVTRWEYASDHRDSRFRGNLLA
                     KREKARPGSGLLIVERGWEWTYHKDIALPIEAVVYQIENGGAKRVLLRQSYEYDPTNL
                     EVSIERVGDTETRIVRNRYGLPVVIWDGRGCATVHRYYSRFNEGLASEGNGGLLAETI
                     ADVTQSTLTQALRVAGVSLLRDERLPERQAEGKPCQRTSRYGYDWLGRRVKEIYPSYT
                     VYHVLNKRGLRLALLDTRSDLTVYDYDAALRLVAQWQRFTPLLLAVYRGEQRVDTPGH
                     FVVQKFRYDSLGRMVAWQPTLERFGIERNEPVEVKYEYYASGVLRKRITPGGTAVEIE
                     YDTTSGKPKAFLLIARNAPEEKLILRQAMRYDPEGFLVGYKDARGATYEQKPDGFGRL
                     YAVVRPDGVRTETLRDGLDRPVQERVLSSDGKVLHETQYEYDQAGRLVCSREHRLAKG
                     IDEWLIAEENRYDAEGNVIGRRSYRDDAWHTFAYDGLGRIVRSQSPTGECSEIYYRDD
                     AVCVETIHRVDTRDGQIYKMRFVTLRDEHGNPWCRIPVGHDGLVGLRRAQLTEHDSFG
                     RIVFEAQPDQPGILRVFNTLGSVDRELTRSASNKVLQE"
     gene            complement(3208..>5055)
                     /locus_tag="PKI05_03675"
     CDS             complement(3208..>5055)
                     /locus_tag="PKI05_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51329.1"
                     /translation="KGQTGMLELSSKDEWVVYSWRPDENPADPKVVGRHPTILRAVVL
                     PAPPHGQRVEDIVNVLETSVKLPSRRGVDPGLWLEIPADWISPRPSSSKPAGKSAKPQ
                     SRRWSTGVMIGIFSLIGVLVVAAGGWSLGLFFGLESGKGQNAPKIPSRDVYEQLLRPL
                     GIEMSEKDRADATLVRLKFREVYCQSWFIDRFFPGASATLGDKSPEELLTEIEDILTK
                     LNDHSNRDEDLQSSSHPDSEFVWVNRDVIRKSLESYSEFTKVIKYLPRHATDDAKTSV
                     TKAVNGVTMGIPSDVRDRVLKIPDSTAWGSVSLDTLSKCVVKPAQEFMNLGQLDYVEC
                     FTTYEWRPGEAFPWDQESDAECQQLVYLLLPQQQFPSDVREAAEEMRIKLCEEPWNVR
                     GITEQDVERRPWFVGRCFVEFLSRKHFTAEEQKLISESQGFVFEHLRKLPISGWDTSG
                     QDGYAGDWRQSWNEVRKRLEELAKSLGVDSGGSEVARVQEVVKVVKKWEQERREGGKK
                     TSARQEKIGNLRKERAKLEERRQEILRQKQEIDASDPRDDELDKEESEVKEKIQKLDE
                     QIRNESAQADANHFSIPEGKVGDFWRRLTGSQYFGDASEGLAESDKKEG"
BASE COUNT         1084 a         1543 c         1202 g         1226 t
ORIGIN      
        1 tttcttggag gaccttgttg ctggcagacc gtgtcagctc ccgatccacg ctgccgaggg
       61 tgttaaaaac tcgcagtatt ccaggctgat cgggctgggc ctcaaagaca atccgtccaa
      121 aggagtcatg ttcggtcaac tgggcacgcc gcaaaccaac gagtccatcg tgcccgactg
      181 gtattctaca ccacggattg ccgtgctcat ctcgcagtgt gacgaaacgc attttataaa
      241 tctgtccatc ccgcgtatcc acgcgatgga tcgtctccac acacacggcg tcatctcggt
      301 agtaaatttc cgagcactcc ccagtggggc tttgcgatcg aacaatccgt cccagcccat
      361 cgtaggcaaa ggtgtgccat gcgtcgtctc gatatgaccg gcggccgatg acgttaccct
      421 cggcatcgta ccggttttcc tcggcaatca gccactcatc aatgcctttg gccagtcgat
      481 gttcgcgaga acaaaccaac cggcctgctt ggtcatactc atactgcgtt tcgtgtaaga
      541 cttttccatc agagctgaga acacgctctt gcacaggccg atctaggcca tcccttagcg
      601 tctccgtcct taccccgtcg ggtcgcacaa ccgcgtaaag acggccgaat ccatcaggct
      661 tttgctcgta tgtagcgccg cgggcatctt tatagccaac aagaaaacct tcaggatcat
      721 agcgcatcgc ctgacgcaga atcagcttct cttccggtgc attgcgcgca atgagcagga
      781 aagctttagg ttttcccgac gtggtgtcat attcaatttc cacagccgtt ccgccaggag
      841 taatcctttt tcttagtact cctgaagcat agtattcata cttcacttca accggctcat
      901 ttcgttcgat cccaaagcgt tcgagtgtgg gctgccacgc taccatgcgg ccaagcgagt
      961 cgtacctgaa tttttggacc acaaaatggc cgggtgtgtc cacccgttgt tccccacggt
     1021 aaacagccaa gagcaaagga gtaaacctct gccactgtgc aacgagcctt aaggccgcat
     1081 cgtagtcata gaccgtgaga tcggagcggg tatcgagaag cgccaacctc aatccgcgct
     1141 tattgagcac atggtaaact gtgtagcttg ggtagatctc cttgacgcgc cggcccagcc
     1201 aatcataccc gtatcgactc gttcgttgac acggtttgcc ctcggcctgg cgttccggca
     1261 gacgctcatc tctcaacaga gatacccctg ccacacggag agcttgagtt aatgtgctct
     1321 gcgtgacatc cgcaatagtc tcggctagca gaccgccatt cccttcactg gctagccctt
     1381 cgttaaaccg agaataataa cgatggaccg tggcacaacc acgcccatcc caaatgacca
     1441 ccggtaatcc ataccggttt cggacaattc gtgtttccgt atcaccaacg cgctcaatgc
     1501 taacttccag gtttgttgga tcgtattcgt aactttgacg caacaaaacc cgcttggcac
     1561 ccccattttc aatctggtaa acaaccgcct caatgggcag tgcaatatcc ttgtggtaag
     1621 tccattccca ccccctctcc acaatcagca aaccagatcc ggggcgagcc ttttcccgct
     1681 ttgccagtaa attgccccga aagcgcgaat cacgatgatc gctagcgtat tcccatctgg
     1741 tcacttgccc gagcggagag cgggcctcga gcttctggcc ggccgcgttg tgtacccagc
     1801 tccatgttgc gttctgcgaa ggaatttccc ggcacaatgg gtaacatcct ggccacctgc
     1861 cgatttctgg aaaagtccat cgctcttccg gaagggggag aggttgtttg taaatgacgg
     1921 tccaggtctt ttccttatga atggtttcga atcgccactc cacaaccgag ggcgtgtctc
     1981 cagcaccccg gaggacgatc cctcccacac gtccgtatgc gggcgcattc gaatcagtct
     2041 cgtacgaata gtgcttctct ggttctcctt tggggggaat aatggctacg agccgtgtcc
     2101 gcccgtccag tccggtgccg taccgatact cccacgttac gtccgctgtc ttggtcaact
     2161 cggtcgcaac tcgcggtaac acaacccgcg tcagccgtcc tagttcgtca tattggtaca
     2221 agacagcaag ctggccgcat acaactccgg tgagttttcc agacgggtca tatccaaact
     2281 gaagacggtt accatacgcg tcctccacca cctggagtcg atctgatccc ggccaatacg
     2341 taaaccgtat gacattcgcc cgccactgat cgtgtcggga tgcgatggac gcgatgcgcc
     2401 aacgccctcc acccacggag ctggacgtag ctgtaaacgt tagacgcacg cggagcgggg
     2461 tttctattgt gatttgatta tcgtttaagg taagttggta gaaagcccct ggttcaggct
     2521 cccaggttcc atttttccat tcaaagcgaa tcggccgccg accagtgtac caaatcagcg
     2581 ctgtaggctt acttggatta ccttccgcaa cgattcgctg gttacaatta tggtcccagc
     2641 cggggcccag tgggccatcg taacccacgt gagaactgta gtatcggacg aatgacagtc
     2701 ccagaccgcc gcggtccggt agtgaaatat cggtctcctc caaaagcaat tcccctgtgc
     2761 caacgactac ctcgttatcg ggcccaccac ggggcgacgg tgctgcgtca gtttcgtggg
     2821 ggactgcctc gccgtttacc gccaggcgat tccacggctc aaacatgcgg catgctctgc
     2881 ccacaccgat gacgggcatc cgatcggcaa caccttccaa ctgggatact cgcttaccca
     2941 aagcctcagc ctcggccaat gcccgctggg cactccacat tagtcgccac tccagcggaa
     3001 cttggcttcg caagtaagcg gttgccccgt tggccacttg gcggtagatg tcctctgggg
     3061 aggaattagg gttcagccgc cactgtccgt cgccggcccg tggcaactcg accgcatctg
     3121 taacctgcgc agtggggtat ttgcttgggg catagaccaa cagtgtcaag gcaacgagcg
     3181 atagccacgt cttaatacgt aacattgtca gccctccttc ttgtcggatt cagcgagacc
     3241 ttccgacgca tcgccaaaat attgcgaacc cgtcaaccgc cgccaaaaat cgccgacctt
     3301 tccttctggt atcgaaaagt ggtttgcatc cgcttgggcg ctttcgtttc gaatctgttc
     3361 gtccagtttt tgaatcttct ctttcacctc actttcttcc ttatctaact catcatcacg
     3421 ggggtccgat gcatcaatct cctgtttctg gcgcaggatc tcttgcctcc gttcctccag
     3481 ctttgcgcgt tcttttctta gattgccgat cttttcctgc ctggcagacg tctttttccc
     3541 accctcacga cgttcttgtt cccatttttt tacaaccttc accacttcct ggacccgcgc
     3601 tacctcggag cctccagaat ccacccccag gcttttcgcc agttcctcaa gccttttacg
     3661 aacttcattc catgattggc gccagtcccc ggcgtaccca tcctgtccgc tggtgtccca
     3721 gccgcttatc ggaagttttc taagatgttc aaagacaaag ccctgcgact ccgagataag
     3781 cttctgctcc tcggccgtaa aatgcttgcg ggagaggaat tccacaaaac atcgcccgac
     3841 aaaccacggg cgtcgctcta catcttgctc ggtgattccc cgcacattcc agggttcttc
     3901 acatagtttg attctcatct cctctgccgc ctcgcggacg tctgaaggaa actgttgttg
     3961 tggcagtagc aggtaaacta attgctgaca ttcagcatcc gactcctgat cccagggaaa
     4021 agcttctccc ggccgccact cataggtagt aaagcattca acgtagtcca attgacccag
     4081 attcataaac tcttgcgccg gcttgaccac acacttggac agtgtgtcta aagaaacgct
     4141 accccatgct gttgagtcgg gtatcttcaa aacgcgatcc cgcacatcgg acgggattcc
     4201 cattgtcacc ccatttacgg ccttggtcac gcttgttttg gcgtcgtcag tagcgtgccg
     4261 cggcaagtac tttatcactt ttgtgaattc cgaataactc tccaggctct tcctaatgac
     4321 gtctctgtta acccaaacaa attcagaatc cgggtgactc gacgattgta agtcctcgtc
     4381 gcggtttgaa tggtcgttaa gtttcgtgag gatgtcttca atttcggtca aaagctcttc
     4441 aggggatttg tctcccaagg tcgcacttgc gcccggaaag aatcgatcaa tgaaccagga
     4501 ttgacagtac acctcgcgga acttgagtct taccaaagtc gcgtctgcac gatctttctc
     4561 tgacatctcg attccgagcg gccgcaaaag ctgttcgtaa acatctcgag aaggtatttt
     4621 tggagcgttt tgccctttgc cactttccag cccgaaaaag agccccaggg accaaccacc
     4681 tgcagcgact acgagaactc cgatgagtga aaaaatccct atcatcacgc cagtactcca
     4741 tcgcctgctt tgaggttttg ccgacttgcc agcaggtttt gacgaacttg gccgcggact
     4801 gatccagtcg gccggaatct ctagccaaag ccccgggtcc accccacgcc gggaaggcag
     4861 tttcacagac gtttccagca catttacgat gtcctccacc ctctgaccgt gtggtggggc
     4921 gggaagaacg accgcccgca gaatagtagg gtgtcgccct accactttgg ggtccgccgg
     4981 gttttcatct ggtcgccagg aatacaccac ccactcatct ttgctagaaa gctccagcat
     5041 cccggtttgc ccctt
//
LOCUS       DAOPTR010000127         5051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_13430_length_5051_cov_4.453963, whole genome shotgun sequence.
ACCESSION   DAOPTR010000127 DAOPTR010000000
VERSION     DAOPTR010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13430_length_5051_cov_4.453963"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PKI05_03680"
     CDS             <1..231
                     /locus_tag="PKI05_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51330.1"
                     /translation="DQEYDLFVAAKDKLGRVFEAWKRVSQLRRDIQDLKESLKDAREE
                     LEMAEARFGALMNEVMDGGAAELEDAASYGRS"
     gene            218..523
                     /locus_tag="PKI05_03685"
     CDS             218..523
                     /locus_tag="PKI05_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51331.1"
                     /translation="MEDREKLFYRNLIDSWKDYIVEYHCVLLLWKGFKIDEVMDVTGL
                     DRSVVGEYQQKIERLQNLAGNLADVLGPSPSQVWNRAQLLRYMRERKVRKVRNAGVG"
     gene            524..1468
                     /locus_tag="PKI05_03690"
     CDS             524..1468
                     /locus_tag="PKI05_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51332.1"
                     /translation="MDRIWECWVADWLNAVEREHGMGDRAIDMLDGLEIDLPLQQAAA
                     PEERRFFELLRQSQESLLEGDFQKALAYGLTAAKVQVASWQAPAWEKPDLGALSQSGV
                     SDEQICWILGWKDEHGNPALEKLREERRRPGLHLDRWQPPVVKEFEERKEAMLKRIEA
                     LRKRLGLRFADEVVVVPLPGVSKKTWELLQAFGKLQRLGWSIRKIARHLGLSYAKTLK
                     LRDQWEAVYGKASQSGTDRAGGGVVAEGVDDQSDSAGVELSPQDDFGVGQEVAGAVAS
                     GGFDSPGGDCGPVAAVEGGGLEGGASASGRPAADCLDD"
     gene            1419..1613
                     /locus_tag="PKI05_03695"
     CDS             1419..1613
                     /locus_tag="PKI05_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51333.1"
                     /translation="MGLQHPGVQRLIAWTIEQEARIAGVYQQPKDTNTTEIRVAGHTP
                     EQVREMLLERVRRLLGPSDN"
     gene            1619..3082
                     /locus_tag="PKI05_03700"
     CDS             1619..3082
                     /locus_tag="PKI05_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51334.1"
                     /translation="MDTCLVEQITELIDSGYCRFRPRPDRPELFDEQSSFVYEQLPGV
                     AVLIGGNGSGTTTAAAYKLARFVLSVQPPPRRDTPFWIVSETFEQVCGVVWQEKLLGL
                     GMIPECEIEWEGISWLDKKRGWPKAVRLKPWPNHPGKNWILEFKSFAQGERGLQARSI
                     GGFLFSEQFHPDLLIEALRGCREYMFAGSCFAEFTPTDPELCLWVESIIDNPPPGWKV
                     YRLNTECNTAISREWLQIFKASIPREMWDTRLTGALPTWEGAIFETFSRAKHVIPRES
                     VTFQRFGWRFYGGIDWGASREHPFAAVIGARHVDGNWIVFEEYWNNTPHTTMTDHLNA
                     LKQLCDRLRVSPVFFADPSRPDCIAQAMEMGFQVFPVPRAGVRSVLLESIDLVRTLLG
                     GDESGSPKLKVTENCRHCVEELRRYRWKRPPRGVWQNVAPPEPVKREDDTVDALRYMI
                     WGATRELRTGNVVTERVRPRLRTVPEVSEKREGWFER"
     gene            3079..4071
                     /locus_tag="PKI05_03705"
     CDS             3079..4071
                     /locus_tag="PKI05_03705"
                     /inference="COORDINATES: protein motif:HMM:NF025005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HOA51335.1"
                     /translation="MTPEQKRILKQFRSGELWQTIRQWEGYARQISRGGREAERAAAE
                     FLKLVQRGGASQFLEKAARFIYARAQDEVLRYGLGDVWKGVRMVIEGFASLEPLVKAA
                     LGPEQRELNLAMRLIEAFSDQPDVLNWAARVLRGKGFRIIPEGQDLNRVRPYPVEPVA
                     GGKVVIRGKTFPPDHPVVTGEMVRARGSSNVYSFGYDAESGSLFIRFRPSPPPGSGTR
                     AKPDSPGPLYQYFHVPLEVFLALLEAPSKGKAVWDLIRIRGTVSGHRYDYTLAGIVGG
                     YVPRKAVYMGGGEEWYLRRRVVGESGREYVSPLPDMPVRGLPNRGLPGRGGGSL"
     gene            4068..>5051
                     /locus_tag="PKI05_03710"
     CDS             4068..>5051
                     /locus_tag="PKI05_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51336.1"
                     /translation="MRIREWIDGWARKVFPAPEENPGARAEGQGAIRYQREVPAMLEV
                     VARHPGGGTLFRLPDNLGQFPVPHVFTFSGVTDLASRIYRPSDEALRASLANARYMLN
                     DPTITECLEQRYRATCLLDWSIEPPEGSGDAEAEIARILGRLLRKIPRFMQYRESLLR
                     AIWYGRAAVVHRWGWTTVEGRPYLTVVSWYPLSGDKLVFRWDENARREELGIKLNPAT
                     PMMSDAGPSLLREAIRSQTEVTSMGPAYFLKPWERSLVAVHKHILEDAPYECPEMAGS
                     RHGIGIRSRIYWLWYQKQEILSFLMEFLERSAFGFEIWGYPAGSKEAEERVR"
BASE COUNT          931 a         1228 c         1860 g         1032 t
ORIGIN      
        1 gaccaggagt acgatctttt tgtggcggcg aaagacaagt tgggtcgggt ctttgaggcc
       61 tggaagcggg tttcccagtt gcgacgggat attcaggatt tgaaggaatc tctcaaagac
      121 gcccgtgagg aattagagat ggcggaggct cgatttggcg ccctgatgaa cgaagtcatg
      181 gatggtggag ccgccgagtt ggaggatgcg gcatcctatg gaagatcgtg aaaaactgtt
      241 ctacaggaat ctcattgatt cttggaaaga ttatattgtt gaataccatt gtgtgttact
      301 tttatggaaa gggtttaaga ttgatgaggt gatggatgtc acaggattag acaggtccgt
      361 tgttggggag taccagcaga agatcgagag gcttcagaat ctggcgggaa acctcgccga
      421 tgtgttgggt ccgagtccat cacaggtgtg gaacagggca caactgctgc ggtacatgcg
      481 tgagcggaag gtgcggaagg tcaggaatgc aggagtggga tgaatggacc gcatatggga
      541 gtgttgggtg gcggattggc tgaacgccgt ggagcgggag cacgggatgg gtgatcgggc
      601 catcgacatg cttgacggtc tggagatcga cctgccgctt cagcaggcgg cggccccgga
      661 ggagcgaaga ttctttgaac tgcttcgtca gagtcaggag tccttgttgg agggggattt
      721 tcagaaggcg ttggcctacg gtttgacggc ggccaaggtg caggtggcat catggcaggc
      781 cccggcgtgg gagaagcccg atcttggagc gttatcccag agcggggtca gcgacgagca
      841 gatttgctgg attctggggt ggaaggatga gcatgggaat cccgctttgg agaagctgcg
      901 ggaggagcgt cgccgcccgg gcctccatct tgacaggtgg cagccgcccg tggtgaagga
      961 gttcgaagag cggaaagaag cgatgctgaa gcggatcgag gccctgcgga agcggctggg
     1021 gttgcggttt gcggacgagg tcgtggttgt tccgctgccg ggggtttcga agaagacgtg
     1081 ggagttgctg caggcctttg ggaagttgca gcggttgggg tggtcgattc ggaagatagc
     1141 ccggcatttg gggctgtcgt atgcgaagac gctcaaatta cgcgaccaat gggaggccgt
     1201 ttatggcaaa gcatctcagt ccggaacaga tcgagcgggt gggggagttg tggctgaagg
     1261 ggtggacgat cagtcagata gcgcgggagt tgaattatcc ccacaagacg atttcggtgt
     1321 gggtcaggaa gttgcgggag cagtggcatc aggaggtttc gattctcccg gcggagattg
     1381 tggcccagtt gcggcagttg agggcggagg cctggaaggt ggggcttcag catccgggcg
     1441 tccagcggct gattgcctgg acgattgagc aggaggcccg gattgccggg gtctatcagc
     1501 agcccaagga tacgaacacg acggagattc gcgtggcggg gcacacgccg gagcaggttc
     1561 gggagatgct cctggagagg gtccggcggt tgcttgggcc gagtgacaat tgagccgtat
     1621 ggacacgtgt cttgttgagc aaatcaccga gttgatcgat tcagggtatt gtcggtttcg
     1681 tcctcgtccc gaccggccgg aactcttcga cgagcagtcg tcctttgtct atgagcagct
     1741 tccgggggtg gcggtcttga tcggggggaa cggttcgggg acgaccacgg cggcggcgta
     1801 caagctggcc cggtttgtgc tttcggtgca gccgccccct cggcgggata ctccgttttg
     1861 gattgtctcg gagacctttg agcaggtctg cggggttgtt tggcaggaaa agttgctggg
     1921 gttggggatg atcccggagt gcgagatcga gtgggagggg attagctggc tggacaagaa
     1981 gcggggctgg ccgaaggcgg ttagactgaa accctggccg aatcatcccg gcaaaaactg
     2041 gattctggag tttaagagtt ttgcccaggg ggagcgggga cttcaggccc ggtcgattgg
     2101 cggttttctc ttttcggagc agtttcaccc cgatcttctc attgaggccc tccggggctg
     2161 tcgggaatac atgtttgcgg gatcctgttt tgccgagttc acgccgaccg atccggagct
     2221 ttgtctgtgg gtcgaatcga tcatcgacaa tcccccgccc ggttggaagg tctatcggct
     2281 gaacaccgag tgcaacacgg cgatttcgcg ggagtggctg cagattttca aggccagcat
     2341 cccgcgggag atgtgggaca cacggcttac cggggcgttg ccaacgtggg agggggcgat
     2401 cttcgagacg ttcagccggg ccaagcatgt catcccgcgg gagtcggtga cgtttcagcg
     2461 tttcggctgg aggttctacg gcgggatcga ctggggggcc agtcgggagc acccttttgc
     2521 cgcggtgatt ggggccagac atgtggacgg taattggatc gtttttgagg agtactggaa
     2581 taacacgccc cacacgacga tgaccgatca tctgaatgcc ctgaagcagc tctgtgaccg
     2641 gctgcgggtc agtcccgtct ttttcgccga tccgagtcgt ccggattgca tcgcccaggc
     2701 gatggagatg ggtttccagg tgtttccggt gccgcgggcg ggggtgcggt cggtcctgct
     2761 ggagagcatt gatctggtca ggacgttgct gggcggcgat gagtcgggga gtccgaagtt
     2821 gaaggtcaca gagaactgcc gccactgtgt cgaggaattg cggcggtatc gctggaagcg
     2881 gcccccgcgg ggggtgtggc agaatgtggc gccgcccgag cccgtgaagc gggaggacga
     2941 cacggtggac gccctacggt acatgatctg gggggcgacc cgcgaactgc ggacggggaa
     3001 tgtggtcacg gaacgggtcc ggccaaggtt gcggacggtc cccgaggtga gcgagaagcg
     3061 ggaagggtgg tttgagcgat gacgccggaa cagaaacgca tcttgaagca gttccgcagc
     3121 ggcgaactgt ggcagacgat tcgccagtgg gagggttacg cccgtcagat cagccgggga
     3181 gggcgagaag cggagcgggc cgcggcggag tttttgaagc ttgtccaaag aggcggggcc
     3241 agtcaattcc tagaaaaggc cgcacggttc atctacgccc gggcccagga tgaggtcctc
     3301 cgctatgggc tgggggatgt ctggaagggg gtccggatgg tcatcgaggg gtttgcgagt
     3361 ttggaacccc tggtcaaggc cgcgctgggg ccggagcagc gggaactcaa cctggcgatg
     3421 cgcctcatcg aggccttttc cgatcagccg gatgtactca actgggcggc gcgggtcctc
     3481 cggggcaagg gatttcggat catcccggaa gggcaagacc tgaatcgggt tcggccttac
     3541 ccggtggagc ctgtggcggg gggcaaggtc gtcatccgcg ggaagacgtt tccccctgat
     3601 catccggttg tcacggggga gatggtccgg gccagagggt cctccaacgt ttacagtttc
     3661 ggctacgatg ccgagagcgg ttcgctgttc atccgtttca ggcccagtcc tcccccggga
     3721 tcggggacgc gggccaagcc ggacagcccg ggaccgctgt atcagtattt tcacgtcccc
     3781 ttggaggtct tcttggccct gttggaagcc cccagcaaag ggaaggcagt ttgggatttg
     3841 atccgtatcc gggggacggt ttccgggcat cgttatgact acaccctggc ggggattgtt
     3901 gggggctatg tcccccgcaa ggcggtttac atgggcggcg gcgaagagtg gtatttgcgt
     3961 cggcgggtcg ttggggagag cggtcgggag tatgtgtctc cccttcccga catgccggtg
     4021 cgtggattac cgaaccgcgg cctgccgggc cgtggtggag gttctctatg aggattcgcg
     4081 aatggattga cggatgggcg cgaaaggtct ttccggcccc cgaagagaat ccgggggcaa
     4141 gggctgaggg gcaaggggca atccgctatc agcgggaggt cccggcgatg ctggaggtgg
     4201 tggcccggca tcccggcggg gggacgctct ttcggctgcc ggacaacctc gggcagtttc
     4261 cggtgcccca tgtcttcacc ttcagcgggg tcacggattt ggccagtcgg atttaccggc
     4321 cttcggacga ggccctccgg gcgtcgctgg ccaacgcccg ctacatgctc aacgacccca
     4381 cgatcaccga atgcctggag cagcgctatc gggcgacgtg ccttctcgac tggagtattg
     4441 aaccgcccga ggggtcagga gatgccgaag cggaaatcgc aaggattctt ggccgactgc
     4501 ttcgcaagat tccgcggttc atgcagtacc gggaatcgct gttgcgggcc atttggtatg
     4561 gccgggcggc ggtggtccac cgctggggct ggacgacggt tgagggcaga ccttacctga
     4621 cggttgtcag ttggtatccc ctcagcggcg acaagctcgt cttccggtgg gacgagaacg
     4681 cccggcggga agagctgggg atcaagctca atccggcgac gcccatgatg agcgacgccg
     4741 ggccgagtct cttgcgggag gcgattcggt cccaaacgga ggtgacgagc atgggaccgg
     4801 cctatttttt gaaaccctgg gagcggtccc tggtggccgt ccacaagcac atcctggagg
     4861 acgcccccta cgagtgcccg gagatggccg gttcccggca cgggatcggg atcagaagcc
     4921 gcatctactg gttgtggtat caaaaacaag agattctgtc atttttgatg gagtttctgg
     4981 agcggagtgc ctttgggttt gagatttggg gctatccggc gggatcgaag gaggcggagg
     5041 agcgggtccg g
//
LOCUS       DAOPTR010000128         4984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_13644_length_4984_cov_3.198012, whole genome shotgun sequence.
ACCESSION   DAOPTR010000128 DAOPTR010000000
VERSION     DAOPTR010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13644_length_4984_cov_3.198012"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PKI05_03715"
     CDS             <1..467
                     /locus_tag="PKI05_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51337.1"
                     /translation="AKLLEGYQILSELGLPLYITEITIPSAGEQGEEIQAELVRNLYR
                     LWFSVPMMAGITWWNLADGTAVQGENEARGGLMTEDLQPKPAYRELERLIHQEWKTKA
                     EGHTDQAGTYRVRGFHGRYLVRVGKENTWVVRTLTVDQNNPAAMEVVWQENE"
     gene            936..1679
                     /locus_tag="PKI05_03720"
     CDS             936..1679
                     /locus_tag="PKI05_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51338.1"
                     /translation="MVTSRQIVRQKAIELLEKAPDGIRFSDLVRAIEAECPDIPRGTI
                     AGSLQDWDKGEPEKVYKPARGLFRHTRFREEVAPAKEDVPPAGTQAPSEESFYEPFAE
                     WLVRELEECTKAIPLGRNKFKDKWGTPDVIGIREPRKSDIIKPPTEIVSAEVKLDTAN
                     VITAFGQACSYKLFSHKSYLVIPSDSPEEDLARVDALCRIFGIGLILFDRRKPESPNF
                     EIRVRAAKHEPDMFYANKYLRFVEEDLFR"
     gene            complement(2317..2389)
                     /locus_tag="PKI05_03725"
     tRNA            complement(2317..2389)
                     /locus_tag="PKI05_03725"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2354..2356),aa:Ile,seq:cat)
     gene            2810..3574
                     /locus_tag="PKI05_03730"
     CDS             2810..3574
                     /locus_tag="PKI05_03730"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOA51339.1"
                     /translation="MEIHILGSGGYHPSSKRHTLCSVIPQAGVILDAGTAFFRLGRFL
                     DHKPWHVFLTHAHLDHVVGLTYYWGLAQQKEVGPVKIWADESTLKAIKDLLFSQPLFP
                     KIPPYEFIPVSPNESVAIATERAGCESVSVLPVPLSHPGGALGYRFQIGDRSVAYITD
                     TTLEALPVYGQVIQDVDLLIHEAFYPAGYEEMAQRTGHVTVAQAAEVARQVRAKRLVI
                     VHLNPIVDCDGEKEQDEVARIFSNTVIATDNLVVNI"
     gene            complement(3889..>4984)
                     /locus_tag="PKI05_03735"
     CDS             complement(3889..>4984)
                     /locus_tag="PKI05_03735"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOA51340.1"
                     /translation="EAAKTPNLDRLASRGVCGLMTPIKPGITPGSGPGHLGLFGYDPL
                     KYVIGRGVLEATGIGFAVGPKDVAIRCNFCTLDAEGRITDRRAGRIPSEESAAVVSLL
                     KAIRIPGVEVFVEPVKEHRFVVVFRGEGLGANVADTDPQVVGVPPLDPKPLDEESKRT
                     AEIAKQFVTEARKILANQPKANGLTMRGFSKRPDLPTYAEAYGLKAAAIAVYPMYKGL
                     ASLVGMDLLGSPTTLDEEITTLEQAWRETDYDFFFVHFKYTDSRGEDGDFDAKVKMIE
                     QFDAVMPRVEALRPDVLIVTGDHSTPAKLKSHSWHPVPTLLVADTCRPDGAKEFSERA
                     CLYGGLGHFEAVNLMPLALAHAGRLAKFGA"
BASE COUNT         1129 a         1287 c         1466 g         1102 t
ORIGIN      
        1 ctgccaaact cctagaaggt taccaaatcc ttagtgaact tggtttgccg ctttacatta
       61 cggagatcac gatcccgtcc gctggggaac agggcgagga gattcaagcc gaactggtgc
      121 gaaatcttta tcgattatgg tttagcgtcc cgatgatggc cggtatcacc tggtggaatc
      181 ttgccgatgg cacggccgtg cagggagaaa atgaggctcg gggcggcttg atgacggagg
      241 acttgcagcc caaaccggcc taccgcgaac ttgaacggct tattcaccag gagtggaaaa
      301 caaaggcaga aggccacacg gaccaagcgg gcacctatcg tgtgcgtgga ttccatgggc
      361 ggtacttggt ccgtgtgggg aaggaaaaca cttgggtcgt acggacgttg accgtggatc
      421 agaataatcc ggcggccatg gaagtggtct ggcaggaaaa cgaataattg gcttcgagtc
      481 tattccaaaa agtccccctc tccctccggg agagggttgg ggtgagggga agcgatcgtc
      541 ggagtcgatc gagaaaaccg gcagcgattc gagccactct cacccgcgcc ccctctcgcg
      601 cccaacgggt gacgggaatt atggaatggg ctcttactaa accggctgaa agagcagctg
      661 ccgaaaagag cacgagaaaa attcgccggg ccgtttggtt tgccccgaga tcaaaggagt
      721 ttcccagaaa acacgtcggc tttgcgcgtg ggcagttgta caaatggtgc gcacgctgag
      781 aaacaccgat ggaaagcttc accaggtatt ctagccgctg ggccggagca agtcacggcc
      841 aatactagcg taagccaatg ttgatgacta tacttacggc tttggaattc gccagccagg
      901 gtaaatgtaa gggatttggt gggcaaggtg gggatatggt aaccagcaga cagatcgtca
      961 ggcagaaagc gatcgagttg ctcgagaaag cgccagacgg tatccgattc tctgatttgg
     1021 ttagggcgat cgaggcagag tgtcccgata ttccaagagg cacaattgct gggtctctcc
     1081 aggattggga caagggggag cccgaaaaag tgtacaagcc tgcacgaggt ttgtttcgcc
     1141 atacacgctt tcgagaggaa gtagcgcccg caaaagagga tgtgcctcct gctggcacac
     1201 aggctccaag cgaggaatcg ttctacgaac cgtttgcgga gtggctcgtc agggagctag
     1261 aagagtgcac caaagcgatt cctttgggaa gaaataaatt caaagacaag tggggaactc
     1321 cggatgtcat cggtatccgt gaaccgcgca aaagcgatat cattaagcca ccaactgaga
     1381 tcgtctctgc agaggtgaaa ctagacactg caaacgtgat cacggcgttt ggtcaagcct
     1441 gttcctacaa actttttagc cataagtctt atttagtgat tccaagtgac tcgcctgagg
     1501 aggatctcgc aagggttgat gctttatgca gaatcttcgg catcggctta atactatttg
     1561 acagaaggaa acctgagtcg cctaattttg agatacgagt gagggcggcg aaacacgagc
     1621 ctgacatgtt ttatgcaaac aagtatctac ggtttgttga agaagatctc tttcggtgac
     1681 agatctcctc agacgaagaa ccatcgtggt tgaccaaata gcgacagcca aacgttgcct
     1741 catggtctac cgtttccatc tgccggcaaa ttgcaaaccg ttagcccgtt tgatgccgcg
     1801 tttttgcaag aatcgcccgc tcctggtgat tgtggggctt ttgccttagg ggcggtaaga
     1861 tgacgccttc cgcggcggaa gcagcacgag cgcgcctgag ttgatcggcg aagccagcct
     1921 gggaatcaaa caccggcagg cacagttttc gagtgcctat cggccgttgc cgtaaccgcc
     1981 ggacgagcag ttgagtgtct gtttcctatc ggtggttgta tcggcaggga gagacttcgc
     2041 tttagccgaa tcctcggtgc cgacgtccag tgggcacgtc atgccgtgtt gcaactgcga
     2101 gattactgtc cactttgtga tgacttagaa tctatcccaa aaagcccccc tctccctccg
     2161 ggagagggtt ggggtgaggg caagcgaacg tcgggatcga tcgagaaaac cggcagcgat
     2221 tcgaggcacc ctcacccccg acccctctcc cgctgaacgg gcgaggggag attatgggat
     2281 aggctcttag caaagtttgt ggctctcatt tacgagtagg cccggaggga ttcgaacccc
     2341 cgaccaaggg attatgagtc ccctgctctc accgctgagc tacgggccca aaacgaatcc
     2401 tttatcgtat agtcttggcc aattcgcgat ttagtcttat gcctcttact cagaatggcc
     2461 tcatcccgtc aattccaagc cgttttgtct tattttatcg ggtagcggaa ccgcagtaac
     2521 aggtccccgg ttaacgtttg atgcttgtgg ctaccatcgt catcattggg gtgctacctt
     2581 ggaaagaata acgaaagttg acgagacgtg atttgggata catcgttgtc cccgggggat
     2641 aatgggcgtt ctgatcgtcg ccaagagtgg ccgaagggtt gtgcccggct gtggcaccga
     2701 tgcccgatgg gacccgagaa tcgccaaaga cacgagagtt gcttcggctg gtggcgcgat
     2761 gcgaaaaggc gagacattga ttgataaggc atcctcatag gggtgaatta tggaaattca
     2821 catcctaggt agcggcggtt accatccaag ttccaagagg cacacgctct gcagtgtgat
     2881 tccacaggcc ggtgtaatcc ttgatgccgg aactgccttc ttccgactcg gccgtttctt
     2941 ggaccacaaa ccctggcatg tctttttgac acacgcccat cttgatcacg tggttggctt
     3001 gacgtactac tggggccttg cccagcaaaa agaggtaggc cctgtgaaga tctgggcgga
     3061 cgagagcacg ctcaaggcta tcaaagatct acttttctcg cagccgcttt ttcccaagat
     3121 accgccgtac gagtttattc cggttagtcc gaacgagagc gttgcaatag ccacagagcg
     3181 ggcgggatgt gagtcggttt ctgtccttcc ggtgccgctt tctcatcctg gaggggcact
     3241 gggttatcgt tttcagatag gtgatcgatc ggtggcctac attacggata caactttgga
     3301 agccctgccc gtctatggcc aggtcattca agatgttgat ctgctcatcc atgaggcgtt
     3361 ttatccggca gggtatgagg agatggcgca aagaactggc cacgtgacgg ttgcgcaagc
     3421 tgccgaggtt gcccgccagg tgagggcaaa gcgattggtc atcgtgcacc ttaatccgat
     3481 cgtggattgt gatggtgaaa aagaacagga cgaggtggcc cgtatcttct caaacacggt
     3541 tattgccaca gataacttgg ttgtaaacat ttgatctcgg atggcgatgt ttctttcgga
     3601 aagatgcggc tcgtctgagt accgacggca aaggagcgat gatctgtcgc ctcggcaacc
     3661 cgggcaagat ggcggcgcag aactcagctt gtccgataat tagctcctcg acgcgcggat
     3721 cgcgcgtaag acgcggaaac ggccagtcct gccagggaat ataaccaaca agcggtacga
     3781 gaccgcgaac gcgatagagc gcgtacctgc agaggggcgg gcgcggagga tcgtgcccct
     3841 cccaaacggc ggtagtggaa gatcacgctc ggccgtggcc ggggcaacct aggctccgaa
     3901 tttggccaat cggcccgcgt gcgccagggc gagaggcatc aggttgacag cctcgaaatg
     3961 gcccagtccg ccatagaggc acgcccgttc actgaattct tttgccccgt ccggtcgaca
     4021 cgtatcggca acaagaaggg tgggaactgg gtgccagcta tggcttttga gcttggcagg
     4081 cgtgctatgg tcgccggtaa cgatcaggac gtcggggcgc aaggcctcga cacgcggcat
     4141 gacggcgtca aactgttcga tcattttcac cttggcatcg aaatcgccgt cctcccctcg
     4201 gctatctgtg tacttaaagt ggacaaagaa gaaatcgtag tccgtctccc gccatgcctg
     4261 ctccagagtg gtgatctcct catcgagggt tgtgggtgat ccgagtaggt ccattccaac
     4321 caggctggct aggcccttat acatcggata aacggctatg gcggcagctt tcaggccata
     4381 ggcttcggca taggtgggca aatcaggccg tttggagaag ccacgcatcg tcaggccatt
     4441 ggcttttggc tgatttgcta ggatcttgcg ggcttcggtc acaaactgct ttgcgatttc
     4501 ggccgttctt ttgctttctt cgtctagcgg cttagggtct agtggcggca cgccgacgac
     4561 ctgcggatcg gtgtcggcca cattggcccc aaggccttcg cctcggaaga ccaccacaaa
     4621 acggtgttct ttcacgggtt cgacaaagac ttcgacccct ggaatgcgga tggccttcag
     4681 caggctaacg acggcggcac tttcctcgct tgggatgcgg cccgcccgac gatcggtgat
     4741 gcggccttcg gcatcgagag tacagaaatt gcatcggatg gccacgtctt tgggaccaac
     4801 ggcgaaaccg attccggtgg cttctagaac accgcgtccg atgacatatt tcagcggatc
     4861 gtacccaaac agtcccaggt gtcccggtcc acttcctggt gtgatcccgg gcttaatggg
     4921 cgtcataagc ccgcacacac cgcgggaggc caggcggtcg agattcggtg tcttcgccgc
     4981 ctcc
//
LOCUS       DAOPTR010000129         4982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_13654_length_4982_cov_3.524457, whole genome shotgun sequence.
ACCESSION   DAOPTR010000129 DAOPTR010000000
VERSION     DAOPTR010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13654_length_4982_cov_3.524457"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="PKI05_03740"
     CDS             <1..1089
                     /locus_tag="PKI05_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HOA51341.1"
                     /translation="GLGPLEPYMQDPDVTDILVNGPFEVYVERCGRLQATNTVFADEE
                     HLLQIIQRVVSRVGRRIDEQNPMVDARLPDGSRVNAVIPPLALNGPVLSIRRFGHERL
                     KIADLIAFGSICQEIVELLRAAVEGRINILISGGAGSGKTTLLNNLSAFIPEDERLVT
                     IEDSAELKLQRKHVVSLETRISNAEQRGEVTPRDLVRNALRMRPDRIILGEVRGAEAL
                     DMLQAMNTGHEGSLTTIHANDTRDALSRLEVMVSMAGFELPVNVVRQYIGSAITLVVH
                     LARLKGGVRRVMRVAEIIGCSNGDYQMQELFGFQQEGVDGAGRAKGHFYATGNVPRFL
                     KRLEEQGIKLPESLFQKRVLTPAITSLE"
     gene            1103..2035
                     /locus_tag="PKI05_03745"
     CDS             1103..2035
                     /locus_tag="PKI05_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOA51342.1"
                     /translation="MAWAVWLYPILTFTAVSGLLILFALMILGRRSKDSRNAEELRPE
                     DLDLEEILYIPPAKAARTALDRWFYELLEAAGSRLDRSTASLLPAAGAVIGAAIPVVF
                     FDSVFWAIVGTVIGAFAPIGFWKFQAFRRVRRMRKQLPVVLQLFADAVRSGLSLERAT
                     ALAAQESKGPLQAEFAWCATQLELGHSPVRVMERMAQRIPIPEFRIFATAALVHRRTG
                     GDLANLADRLARSAQDRTEFYGHLKAVTAGSRLSVFGLTAGVFIAVVLLGLTRPEYIQ
                     QFLNHPSGLSILGIAAGLQVVGLLWVWRILKARY"
     gene            2101..3180
                     /locus_tag="PKI05_03750"
     CDS             2101..3180
                     /locus_tag="PKI05_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOA51343.1"
                     /translation="MPDILLLALGYWQDLAHLWSEWGLSILTFANVVLLAMIIRWILV
                     RRAQPKASAGLPPVVDPETDDPGAFGALTPALAAQIPESAKETRDFQLLLRQAGLHRP
                     SARYTIYAFRFLLLVTPLIIAGVCAVIAPQAWTLPILVAGGLSSAVLSIIPRMYVFLR
                     RRKRYEEIRSGLADTIDMLGMCLTGGLSLLDSLEHVAGQMGAYPALAEELRTVRRQAE
                     VGSLKLALEDLTRRVDLPEVRQLCAVLSRGTQLGSQLASSLSEHADHLRVALKQAALA
                     RANKAPVKLVLPILFCLAPAALIVLTAPAFLELKQFFDPSLMKSASGKGVSFGTNSVV
                     DAIRDLDQRVIFEPPPTEIVPPISQ"
     gene            3486..4826
                     /locus_tag="PKI05_03755"
     CDS             3486..4826
                     /locus_tag="PKI05_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HOA51344.1"
                     /translation="MSQTPMMRPLASTPRTGIESRQLSFDELKQRIHSKLVDKLDLSK
                     IGQLEGEVLRREIRLVVEHLCDSENTMLNRSERERLIDEVLDETFGLGPLEALLKDPT
                     ISDILINGPKNVYVERRGKLEKTNVCFRDNEHLLQIIDRIISKVGRRVDETCPMVDAR
                     MPDGSRFNAIIPPLALDGAAVSIRRFGRSPLKLEDLLNLKAFTPEMVMLLEGAIKARL
                     NIIIAGGTGSGKTTLLNTLSSFIPNNERIITIEDAAELQLQQEHVVRLETRPPNIEGK
                     GAITATDLVRNALRMRPERIIIGECRGPEALDMLQAMNTGHEGSMTTLHANSPRDALA
                     RLETMIMMAGFELPLKAMRTQIASAVNLLIQANRLQGGVRRITHITEVVGLEQDTIIT
                     QDIFRFEQDGVDENGRAYGRFVATGIRPTFMPRLEARGIKLPPSIFRQRTMMEA"
BASE COUNT         1059 a         1382 c         1391 g         1150 t
ORIGIN      
        1 ggcctggggc cactcgaacc ctatatgcag gaccctgacg tgaccgacat cctcgtcaat
       61 ggcccgtttg aggtttatgt cgagcgctgt gggagacttc aggcgacgaa cacggtgttt
      121 gctgatgaag agcatctcct acagatcatt caacgagtcg tctcccgtgt ggggcgccgc
      181 atcgacgaac aaaacccaat ggttgacgct cggctgcctg acggttcccg tgtcaacgcc
      241 gtaattccgc ccctggccct caatgggccg gtcctttcca ttcgccgttt tggccacgag
      301 cgcctaaaga tcgccgacct tattgccttc ggctctatct gccaagagat tgtggagcta
      361 ctccgcgccg ccgtggaagg acgtatcaac atcctcatta gcggcggtgc tggttcgggt
      421 aagacgacac tgctcaataa tctttcggcg tttattcctg aagacgaacg attagtaacg
      481 atcgaggatt ccgcagagct caagttgcaa cgcaagcacg tcgtcagcct ggagacgcgt
      541 atctctaacg ccgaacagcg gggagaagta acaccccgag atctcgtacg aaacgccctt
      601 cgcatgcgtc ccgatcggat catcctgggc gaggtccgtg gtgccgaagc cctcgatatg
      661 cttcaggcga tgaatacggg tcacgaaggg tcgctgacca caatccatgc caacgacaca
      721 cgggatgccc tctcccgttt ggaagtcatg gtcagtatgg cgggtttcga acttccggta
      781 aatgttgttc gccagtacat cggatcggca attaccctcg tcgtgcattt ggcccggctt
      841 aaaggcggcg tgcggcgggt catgcgcgtc gcggaaatca ttggctgtag caacggggat
      901 taccaaatgc aggaattgtt cggctttcag caagagggtg tggatggtgc tggccgagcc
      961 aaaggacact tctatgccac cggcaatgtt ccgcgatttc tcaaacggtt ggaggaacag
     1021 gggatcaagc ttcccgaatc cctcttccaa aaacgtgtct tgaccccggc gattacatcc
     1081 ctcgaatagg aggtttcctc tcatggcatg ggccgtttgg ctttatccga ttctcacatt
     1141 tacggcggtc agtgggctgc ttatcctgtt cgcgttgatg atccttgggc gtcgatcgaa
     1201 ggattcgagg aacgcggagg aattgcggcc agaggaccta gatcttgagg aaatcctcta
     1261 tattccgccc gcaaaagccg ctcgaaccgc gttagaccga tggttctacg aattgctcga
     1321 agccgcagga agccgcctgg atcgttccac ggctagcctg ctaccggcag cgggtgctgt
     1381 gattggtgcg gcaattcccg tggtgttctt tgatagtgtc ttttgggcca tcgtgggcac
     1441 ggtgatcggg gcctttgcgc caatcggatt ctggaagttt caggcctttc ggcgggtacg
     1501 tcgaatgaga aagcaattgc ccgtggtgct gcaactgttc gcggatgccg ttcgcagcgg
     1561 gctctccttg gagcgagcaa ccgcgttggc agcacaagag tcaaaaggtc ccctacaagc
     1621 ggagtttgcc tggtgtgcca cccagttgga actcgggcat tctccagttc gggttatgga
     1681 gcggatggcc cagcgaattc ccatccccga atttcgtatc ttcgctacgg ctgccctcgt
     1741 ccatcgtcgg acgggcggtg acctggccaa cttggccgac cgcttggcac ggtccgccca
     1801 agatcgcacc gagttctatg ggcacctcaa agccgtcaca gcggggagcc ggctctctgt
     1861 gttcggtctg acagcaggag tgttcatcgc ggtggtcctt cttggactta ctcggcccga
     1921 gtacatccag caatttctca atcatcccag tggactttcc attttgggca tcgctgcggg
     1981 attgcaggtc gtcggtctgc tgtgggtctg gcgcatcctc aaagcgcgtt attgaggacc
     2041 atcatgccgt agtgatgtta ccgacgaata tattgaagat tagcgacaaa ggtcacgcat
     2101 atgccggata ttctacttct cgctttaggt tactggcagg acttggctca tctgtggagc
     2161 gagtggggat tgtcgatcct cacgtttgcc aacgtggtct tactggcgat gattatccgt
     2221 tggattctcg tccgccgggc ccagcccaag gcgtccgcgg ggcttccgcc agtcgttgat
     2281 cccgaaactg atgaccccgg cgcctttggg gcgttaactc ctgcgttggc cgcgcagatt
     2341 ccggaatcgg ccaaggaaac acgagatttc cagttgctat tgcggcaggc cggcttgcat
     2401 cgtccctcgg cacgctacac gatttatgcc tttcgttttt tgctcctcgt gacacccctc
     2461 attatcgcag gcgtttgtgc tgtcatcgcc ccgcaagcat ggaccctgcc gatccttgtg
     2521 gccggtggat tgagtagcgc cgttctgtcg atcatcccaa gaatgtacgt ctttttgaga
     2581 cgccgaaagc gctacgaaga gatccggagc ggtctggccg acacgattga catgcttggg
     2641 atgtgtttga ccggtggcct cagtctcctc gatagtctcg agcacgtggc cggacagatg
     2701 ggggcttatc cggcgctcgc cgaagaacta cgcaccgtta gacggcaggc cgaggtgggg
     2761 agcttgaagc ttgccctgga agacttgacg cgccgggttg acctacccga agtccgccaa
     2821 ctttgtgcgg ttctttcccg cggcacgcaa ttgggcagtc aattagccag ttctttgagt
     2881 gagcacgctg accacctccg cgtggccctg aagcaagcgg ccctggcacg tgctaacaag
     2941 gcaccggtaa aactcgtgct gccaattttg ttctgtctag ctccggccgc tttgattgtc
     3001 ttaacggcgc ccgccttcct cgaactaaag caattcttcg atccctccct catgaaaagc
     3061 gccagcggca agggcgttag cttcggtact aattcggtcg ttgatgcgat ccgtgatctc
     3121 gatcaacggg tgatctttga accacccccg acggaaatcg tgcccccgat ctctcaatag
     3181 ttcggaagtc aaacgagcta gtctacccaa tatagggcta cggcccccct aggacgtacg
     3241 acctcacaaa ggttaagtcg tcgtagaata agtggagacc aaaaaccgcc gtgtttcgcg
     3301 gcggttttct cttttcttgc cacctgagga cacgccgtaa gctaaatttt gagtgaaaga
     3361 gcatccaaca agtacttttc ggctgagatc gagttgttgg gacattcaaa gacaatccac
     3421 gggctgatct ccaccggtcg agtggaacgt ttagtcttct cgggaagtaa ggagggagct
     3481 ggaggatgtc acagaccccg atgatgcgac cgttggcttc gacccctcgc accgggatcg
     3541 aaagccgtca actttccttt gatgagctaa aacagcgaat ccacagcaaa cttgtcgaca
     3601 agctcgattt gtctaaaatt gggcagttgg aaggagaagt ccttcgccgg gaaatccgcc
     3661 tcgttgtgga gcatttgtgt gacagtgaaa acacaatgct caaccgttcc gaacgggaac
     3721 gactcatcga cgaggtcttg gatgaaacgt ttggcctcgg cccactggaa gcccttctca
     3781 aagacccaac cattagcgat atcctcatca atggccccaa gaacgtttat gtagaacgtc
     3841 ggggcaagct cgaaaaaaca aacgtctgtt ttcgcgataa cgaacatttg ctgcaaatca
     3901 ttgaccggat tatttccaaa gttgggcgtc gggtggacga gacttgccca atggtcgatg
     3961 cacggatgcc cgacggttcc cgtttcaatg ccatcattcc gcccttggcc ctggacgggg
     4021 ctgctgtgtc tattcggcgt tttgggcgaa gtcctctcaa actggaggac ctcctcaact
     4081 taaaggcttt cacaccagag atggtcatgc tccttgaggg ggccattaaa gcccgactca
     4141 atatcatcat cgccggtggt actggttccg gtaagacaac gctcctcaac acgctttcca
     4201 gcttcatccc gaacaacgaa cgtatcatca cgattgaaga cgctgccgaa ttgcagttgc
     4261 agcaggaaca cgtcgttcgg ctggaaacac gtcctcctaa catcgaagga aaaggtgcca
     4321 ttacggcaac ggacttagtt cgcaacgccc tgcgtatgcg gcctgagcga atcatcatcg
     4381 gtgagtgtcg tggcccagaa gccctggata tgttgcaggc catgaacacc ggccacgagg
     4441 gttcgatgac gaccttgcac gcgaactcac ccagggatgc gctggcccgt ttggagacga
     4501 tgatcatgat ggcgggattt gaactgccgc tcaaggcgat gcggactcaa atcgccagcg
     4561 ccgtcaattt gctcatccag gctaatcgtc tccagggtgg cgtgaggcga attacccata
     4621 tcaccgaagt tgttgggctg gaacaggaca cgattattac ccaggatatc tttcgttttg
     4681 aacaagacgg ggtggatgag aacggtcggg catatggacg ttttgtggcc acggggattc
     4741 ggcccacctt catgccacgg ttggaagccc gcggcatcaa attgccgcca tcgatcttcc
     4801 gccagcgcac catgatggaa gcctaaggca attatgacaa ctcagaaaga aacagtggtt
     4861 ctggatgcct attaacgtaa tggaacgagt gacctcatga cccagatgac aatcctcatc
     4921 atcgttgcgg cgttcgtcgg cgcggcggcc gtcgcttttg cccacggaac ccgacatgtc
     4981 ga
//
LOCUS       DAOPTR010000130         4978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_13669_length_4978_cov_2.932358, whole genome shotgun sequence.
ACCESSION   DAOPTR010000130 DAOPTR010000000
VERSION     DAOPTR010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13669_length_4978_cov_2.932358"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2026)
                     /locus_tag="PKI05_03760"
     CDS             complement(<1..2026)
                     /locus_tag="PKI05_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOA51345.1"
                     /translation="MSHFVPVGVDLGNSRIRVAYLNLQGHLEMLRDERDEVNIPNAVR
                     FKGQDILVGKDALDDGLFFSPETAIWIKRELGSRTYSRAVHGQRLPPEVIQACVLRKV
                     RVELLRQLAASPACVMGVPGYFDEVRRKAVINAGQIAGLRVIDVIDEPLAAALAYANA
                     AGALRLESPGQEETILIFDLGSSALQVALAKLSSSECRILAKDYDVELGGYDFDVTLG
                     EIVAEAMMAAGYPDPRADAATWGRVYRAVVEAKHGLSSQNRIHFVYDYEQAHFELIIT
                     REQYEAATAPLLRRIERLLGRFLERTGVSWSEIDVVLPVGGAVRTPCVVQLLTRLTGR
                     PIRFPINVDEAVARGAALYAAWKLEEARRVETKEAAPEVVTAGSATAFGERRITTFGN
                     LLPYAVGIRHPQERDEETICLVSRATPLPFKVTRRLSLGRRDKNELTLVVAKNELGSW
                     EPYGRLIVRGLPEALSPQSFLELTVECQVNGRLGFHARLFPTAEVLRTEFVVPSALEI
                     EAVERWQRTMETSLTWEGLTQTMASPASETRDSRSRDTTDDSSPVGNAKPVLSREPAQ
                     QETELLPRRRHPRLAAAEAQGRWQPLEIALPPGMADKQSEPKPSPSPGDVYPIAEWPP
                     VSPDQSKGTGSQSQEAGSVRSVLIPRRPRVTREALLEGSPECSAPPTGTAS"
     gene            2376..2963
                     /gene="cyaB"
                     /locus_tag="PKI05_03765"
     CDS             2376..2963
                     /gene="cyaB"
                     /locus_tag="PKI05_03765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HOA51346.1"
                     /translation="MLVEVELKFRVAEMEELARQLSSLATEPEVLKEERDLYFAHPVR
                     DFSQTDEALRLRRSGMHNYITYKGPKIDTVSKTRYELDLPLLDGEDVFKQWCDLLEKL
                     GFRPVAEVIKQRRKIWTQWEGWRVEISLDQVENVGQYVEFEIVCDEERVSQARDALLR
                     LAESLQLTHSERRSYLELLLAERSPTHPPTTNVQG"
     gene            3034..4380
                     /locus_tag="PKI05_03770"
     CDS             3034..4380
                     /locus_tag="PKI05_03770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOA51347.1"
                     /translation="MDRREDHHRANQHHGYNGHWCTDDTRPVFKRHRNFWLLVLHQIV
                     FRVGWTFKTESVVLPAFLDHLAGPGAGVFRGFLPVLNRLGQGILPLAASQAIEASRHK
                     RLLLAITTCLLAIPLSVFALIAVVDDELGHGWEAVSFLLVYFAFSAVYGIYQVVFNTV
                     QGKLIEPEKRGRLMSYSTFWGTFPAILAALCALRPWLRDGGARYDLVFLSAATLFAIS
                     GILCLFLREAKKTVDEETSTQSNPVKKMVTSLRCHPNLRGLWFVTTVFSISLTLTPHY
                     QAFARDHLHIGSGQLFLWVIAQSASVGLFSVLIGGLADRYGNRLTLAVLIFGSTLAPG
                     WSLALFHMPQFAETSVWLTFMALAFSTLVPRVAANYALELVPEEDHPVATALLQFGMT
                     VPLFASPLFGQFGEWWGFEPLLYGAIAVNVTATVATFVLPEPRRRIPPGKVITPEE"
     gene            complement(4429..>4978)
                     /gene="argS"
                     /locus_tag="PKI05_03775"
     CDS             complement(4429..>4978)
                     /gene="argS"
                     /locus_tag="PKI05_03775"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOA51348.1"
                     /translation="IVNANDDAKSNGPELSPEERRRVAEVVGIGAIKYADLSQNRTSD
                     YVFSYDKMLAMTGNTGTYMQYAYARIQNIFAKGGVSAEAIRQFDGKIRLKHPAERALG
                     REILRFPEAIELVVEDYRPNQLTAYLFGLANRFSTFYENCPVLQAENAAERQSRLQLC
                     DLTARVIRQGLELLGIEVVPKM"
BASE COUNT         1054 a         1362 c         1355 g         1207 t
ORIGIN      
        1 cgctcgccgt gcccgtgggc ggcgcgctac attcgggtga accttcaaga agggcttcgc
       61 gtgttaccct aggtctgcgg ggaatcaata cacttctgac agagcccgct tcctgggatt
      121 gagatcctgt gcctttggat tgatcgggag aaactggtgg ccactccgca atcggataga
      181 cgtcgcctgg cgatggagaa ggcttaggtt ccgactgctt gtccgccata ccgggcggta
      241 gtgcaatctc caggggctgc catcgccctt gggcttcggc ggcggccagc cggggatgtc
      301 tccgtcgtgg cagaagctcg gtctcctgtt gggctggctc acgactcagc acgggtttcg
      361 cgttccctac aggcgaagaa tcatcggttg tgtcccttga ccggctgtca cgtgtctcac
      421 tagcaggact ggccattgtc tgggttagcc cctcccaagt taaggatgtt tccatcgtcc
      481 gttgccaacg ttcgacagcc tcgatttcga gggcggaggg aacaacgaac tctgtcctaa
      541 gaacttcggc cgtaggaaag agccgggcgt gaaatcccag ccgaccgttg acctggcatt
      601 ctacggtcag ttccaagaat gattggggcg agagagcctc cggaagcccc cggacaatga
      661 gccgcccgta aggctcccat gacccaagtt cattcttggc aacgaccagg gtcaactcgt
      721 ttttatcgcg ccgtcccagc gataagcggc gagtcacttt aaagggaagc ggtgtggccc
      781 gtgagacaag acaaatcgtt tcctcgtctc gttcctgagg gtggcgaatt ccaacggcat
      841 aaggaagcaa gttcccaaac gtggtgatcc gccgttcccc gaacgcggtg gcacttcctg
      901 cggttaccac ctcgggtgcc gcctctttcg tctcaacacg ccgggcctct tcgagtttcc
      961 atgcggcata taacgccgct ccccttgcaa cggcctcatc gacgtttatg ggaaagcgaa
     1021 ttgggcgacc cgttaatcgg gtcaacaatt ggacaacgca gggcgttcgg acggccccac
     1081 cgacaggcaa aaccacgtcg atttcggacc acgagactcc cgtgcgttcg agaaatcgtc
     1141 ctaaaagacg ctcaatccgc cgcagcaacg gggctgtggc cgcctcatat tgttccctcg
     1201 tgataatcaa ctcaaaatgt gcctgttcgt agtcataaac gaaatgaatg cggttttgtg
     1261 acgacagccc gtgtttggct tccaccaccg cgcgatagac ccgtccccat gtggcggcat
     1321 cggctcgggg atcgggatat ccggctgcca tcatggcctc ggccacaatt tcgcctagcg
     1381 tcacgtcaaa gtcgtaacct ccgagctcga cgtcgtagtc tttcgcaaga atccgacatt
     1441 ccgaggacga aagttttgcc agtgcaacct gcaaggcgct actacctaag tcgaagatga
     1501 ggatcgtttc ttcctgtcct ggtgattcga gccgcaaagc ccctgctgca ttggcgtatg
     1561 caagggctgc cgcaagaggc tcatcgatca cgtcgatgac gcggagccct gcgatttgac
     1621 cggcattaat gacagccttc cggcggacct catcgaagta accgggaacc cccatgacac
     1681 atgctggtga cgcggctaac tgacgaagga gttcgacgcg aaccttgcgc aagacacacg
     1741 cctggatgac ctcgggcggt agccgttggc catggaccgc acgactatag gtcctcgaac
     1801 caagctcccg tttgatccat atagcggttt ccggtgaaaa gaataagccg tcgtccaatg
     1861 cgtcttttcc tacgaggata tcttgaccct tgaaccgtac ggcattcggt atgttcactt
     1921 cgtcccgttc atcacgtaac atttccaggt ggccctgcaa attaagatag gccacccgaa
     1981 tgcggctgtt tccgagatca acaccaactg ggacgaagtg gctcatagag accacacggc
     2041 gggaaaatct tctgctattt ccaagagatc attgccatac ctgacaacca cgtgctccag
     2101 tccacaacaa ggtcgtttcg gatgatcctt taccagaccg gagaataacc tcagcctaag
     2161 tctcattgta accgacggcg tctcgctccc caaagtcagc ttcctttcct gctccgcggt
     2221 gtaggtttat gcagcacgtt ttggtatgat tcacgtgagt tagacgtaag cgagatatgt
     2281 acgttgcgat tcttgggcga gtaacatcgt gctgcccggt tttgcgatgg aagatatcaa
     2341 caatgggcaa tcagggaacc acgaaggcgt cagagatgct cgttgaagta gaactgaaat
     2401 ttcgcgttgc cgaaatggag gagttagcca gacagctttc ctccttggct acggaacccg
     2461 aggtcttgaa agaggaacgc gacctttatt ttgcgcatcc agtgcgcgac ttctcccaga
     2521 ccgatgaagc tcttcgatta cgccgctcgg ggatgcataa ttacatcaca tacaaaggac
     2581 ccaagatcga cacagtttcg aagacccgtt acgagcttga tttgccttta ttggatggcg
     2641 aagatgtttt caagcaatgg tgtgatctgc tagagaaact tggcttccgt cccgttgccg
     2701 aagtaattaa gcagcgccga aagatatgga cacagtggga ggggtggcgg gttgagattt
     2761 cacttgatca agtcgagaat gttggccaat acgttgagtt cgagatcgta tgtgacgaag
     2821 agcgagtgtc ccaagcccgc gatgccttgc ttcgattggc cgaatctctc caacttaccc
     2881 attcggagcg gcgcagttat ctcgaactat tgttagctga aaggtctccg acccaccctc
     2941 caaccacgaa cgtgcagggg tagtaggcca tcctgtgagc cgctgtgtga cggaacgtgc
     3001 gattctgcat caacttgcga ttgtcaaaaa ccgatggaca ggcgcgagga ccatcatcga
     3061 gcgaatcaac atcatggcta taacggtcat tggtgcaccg acgatacaag gcccgtcttc
     3121 aagcgacatc ggaatttttg gctcctggta cttcaccaaa tcgtttttcg agttggttgg
     3181 acgtttaaga cagaaagtgt tgttcttccg gcgttcttgg atcacctggc gggacctggg
     3241 gccggggtct ttcgcggctt cttgcccgtg ttaaaccgat tggggcaggg tattttgccc
     3301 ttggcggctt cgcaagccat tgaggcttcc cgacacaaaa gactgcttct agccatcacg
     3361 acgtgtctgc tagccattcc gctgagtgtt tttgccctta tcgccgtcgt ggatgatgaa
     3421 ttgggtcatg gttgggaagc cgtatctttt ctgctggtat attttgcatt ctcggccgtg
     3481 tatggcatct atcaggttgt cttcaacacg gtgcagggaa agctaatcga gcccgaaaaa
     3541 cgagggcgat tgatgagtta ctccaccttt tgggggactt ttccggcgat cttggccgcc
     3601 ttgtgtgctc ttcggccttg gttgcgcgat ggcggagcgc gttatgacct tgtttttttg
     3661 tcggcggcca cgttgtttgc tatttccgga atcttgtgcc tatttttgcg tgaagcaaaa
     3721 aaaacggttg acgaagaaac ctcgacacag tccaacccgg tgaaaaaaat ggtgacatcc
     3781 ctcagatgcc accctaattt gcgcggctta tggttcgtca cgactgtctt tagcattagc
     3841 ctgacactga cgccccatta tcaggccttt gctcgggacc atcttcacat aggatcgggc
     3901 caattattcc tttgggtgat tgcccaaagt gctagtgtgg ggctatttag tgtccttatc
     3961 ggtggcttgg ccgaccgtta tgggaaccgt ctaaccttgg ccgtcttgat ctttggttca
     4021 acgttggcgc caggctggtc attggcactc tttcacatgc cacagtttgc ggaaacttcg
     4081 gtgtggttga cgtttatggc tctcgctttt tccacactgg tcccccgtgt ggcggcgaac
     4141 tatgccctgg aactcgttcc ggaagaagat cacccggtgg ccacggccct ccttcagttc
     4201 ggcatgaccg taccactgtt cgcgtctccc ctctttggtc aatttggcga gtggtgggga
     4261 ttcgagccgc tgttgtacgg tgccattgcg gttaacgtca ccgccacggt agcaacgttt
     4321 gtcctcccgg aaccacgacg tcgaatacct cccggaaaag tgatcacccc cgaggaataa
     4381 ctggaaaacg ctggaaacta agggacgcac cacaccttgt ggccgcgttc acatcttggg
     4441 gaccacctca attcctagta gttccagtcc ttggcggatg acacgcgccg tcaaatcgca
     4501 cagttggaga cggctttgac gctccgcggc gttttctgcc tgcaaaacgg ggcagttttc
     4561 atagaaagtt gaaaaccggt tggccaggcc gaagaggtac gccgtcaact ggttgggccg
     4621 gtaatcctca acgaccaatt cgatagcctc cggaaatcgg aggatttccc gtcccagtgc
     4681 cctctcggcc ggatgcttga gacgaatctt gccatcgaac tgtcgtatag cctctgcgct
     4741 cactcccccc ttggcgaaga tattttgaat tcgggcataa gcgtattgca tgtaggttcc
     4801 cgtattgcct gtcatggcta gcatcttgtc gtaactgaag acgtagtcgc tggttctatt
     4861 ttgagaaagg tctgcgtact tgatggctcc gatgcccacg acttcggcga cgcggcgtcg
     4921 ttcctcggga gaaagctccg gcccattgga cttggcatcg tcgttagcgt taacgatt
//
LOCUS       DAOPTR010000131         4968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_13710_length_4968_cov_3.030328, whole genome shotgun sequence.
ACCESSION   DAOPTR010000131 DAOPTR010000000
VERSION     DAOPTR010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13710_length_4968_cov_3.030328"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="PKI05_03780"
     CDS             <1..414
                     /locus_tag="PKI05_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOA51349.1"
                     /translation="HIHLKCNQFELLGNALDPSQDAPDWGILIPAMEEFRKVNNAPIV
                     VTLGKDGMLVSDPVPTWIPGVRVSGPTDPTGAGDSATAGAVASLASGATLPEAALVGN
                     LVASITIQQLATTGTARPEELPPRLEMWLEQQAQT"
     gene            512..3046
                     /locus_tag="PKI05_03785"
     CDS             512..3046
                     /locus_tag="PKI05_03785"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOA51350.1"
                     /translation="MSDWKPTITHHLTKWLRRNKPAIRALLGDFLQAIEIEQFLALGT
                     SQNTFYDSTKEDDLTQSWAAEWGQADHIPLAAFPVIVAQFSSWLITAVAVHGLSHWNV
                     PDLPKSPDLFANEVMRWLDEDCARRAGSSGLWHLLDWLGIRLPCIANQGDAHRFFKHL
                     AEWAGQFFEQWHQQTDTWCDLFQPLHTVLVPKKIRLRMGEFYTPDWLAEHILAELDYP
                     GSDASRLFDPTCGSGVFLTAAVRRLIPHLLAPHNGWKTGGRSTGSQSPQTRACPESLA
                     LEVVRGVLKDQIVGMDTNPLAVVAAQANLLLATIHTLWRICPAMRLPLDAVLDHPSPI
                     RCGDLLDSSWETPTANARPMGPGAERQTKPQIQPQFHFLVGNPPWLLWDNLTASMRER
                     LAPLCQELGLFDLSGREARHGGAKRDVGVALVWMATQYLLPGGKMGVVLPATLFRGGN
                     AGRSFRRFTVGPETPLRVLQVEDLSGLPVFPGTIRRTVLFFAEKGFPTRYPVPYYVWH
                     KEAGQREMDNGRLHASRTKLVAVPSCPDDTAAPWSITTESARNIWQKVVGPSEYSAYL
                     GANTAGANGVFWLEIIGHECGLAVVRNCAESGRRATPHFQGPLEPDLLYPLLRWKDVI
                     PAGVASTVILLVQDPLTRKAIPEELLKARYPLTYAYLSQFRDILEARAARRKLHRKEP
                     WYSQYNVGHYTMAPHKVVWRRMDWKFRAVLVGPQPLGELGVRPVVPQETCCFIPVDSV
                     EEGAYLLAILNSRLVREIVQASALPGSRGFASPRILRTLAIPRFDPRVDLHRRLAEIG
                     QKASSTGQVSCDKWLEANNLTDWDRCLAELYGLSPSDYVALCEKSV"
     gene            complement(3074..4330)
                     /locus_tag="PKI05_03790"
     CDS             complement(3074..4330)
                     /locus_tag="PKI05_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51351.1"
                     /translation="MITSESSSEALAAGEQVVRLLHEHRARCREVLSRQQERFRLAEQ
                     ELGDYLGGLAKTVEALRAELSQRESKSSEKILPLLSALDSAVRSIQERGESLRTENEN
                     RSQLYAQLTAELAAMRREMAELRSIKAHLEQQLAISQSEKQTDAEQWQRREAQLRAEF
                     ESEKDRWRAQWNDRESAFNALVIRCSELEEELEKLRRQSDPQASSDLQQRYEMAIVEI
                     RELRQKNAELEKKLAQIEQSGTKPVVTSSDGRILDWEAEKQRILAALEAEAQEDSNPE
                     RAAERIRIQDVIKKTEAALAEKDREIAELRRLLEDQSTSIGSVAVGAAAIERLFDQDE
                     VLREQREHLRQLEEEWKEKLRKAELEISLERAKIARERAVLEEKQRELEEKLAALGPS
                     KSPEEEKNKPSRGRWLSRLGINGSSP"
BASE COUNT          983 a         1436 c         1351 g         1198 t
ORIGIN      
        1 cacattcatc ttaagtgtaa ccagttcgaa ttacttggaa acgcgctcga cccgtcgcag
       61 gacgcgcccg attggggtat cctcatcccg gccatggagg agttccgcaa ggttaataac
      121 gcacctatcg tggtaacgct ggggaaagac ggaatgttgg tgagcgatcc tgtgcccacc
      181 tggattcctg gggtgagggt ttccggaccg acagatccca cgggcgccgg cgatagtgcc
      241 acggctggcg ctgtggcgtc tctggcctcc ggggcaacgc ttcccgaggc cgcactcgtg
      301 gggaatctcg tcgcatctat tacgatccag cagttggcaa ccacgggcac cgcccggccg
      361 gaagagctcc ctccccgttt ggaaatgtgg ctcgagcagc aagcacaaac ataacgccca
      421 cgttaccttg agatttacat cgactgctgc gttgctacgg tatcaccaca tgaattttgc
      481 caatttggtc tgcggcagga ctttatagcc catgtcggat tggaagccta ccatcaccca
      541 tcacctgacc aaatggctgc ggcgaaacaa acccgccata cgggcactgt tgggggattt
      601 tttgcaagcg atcgaaatag aacagttctt agcactcggc acatcgcaaa acacgttcta
      661 tgacagcacc aaagaggatg atcttaccca aagctgggcc gccgaatggg gacaagcgga
      721 ccacattcca cttgccgcat ttcccgtgat tgttgcccag ttttccagtt ggctcattac
      781 ggcggttgcc gtccatggtc tctcccattg gaacgtgcca gaccttccta aatcgcccga
      841 tctattcgcc aacgaagtaa tgcggtggct ggatgaagat tgcgcacgca gggccggttc
      901 gtctggtttg tggcacttac ttgactggct tggcattcga ctaccgtgca tcgccaacca
      961 gggcgatgcg catcgttttt tcaaacacct cgccgagtgg gcagggcaat tctttgagca
     1021 atggcatcag caaacggata cctggtgtga cttattccag cccctgcaca cggtgcttgt
     1081 ccccaagaag attcgtttac gaatggggga gttttacacg cccgattggt tggcggagca
     1141 catcctggcc gaactcgact acccgggatc ggatgcctcg cgcctgtttg atcccacatg
     1201 cggttcgggc gtcttcttga cggcagccgt gcgacgtctg attcctcatt tgctcgcacc
     1261 ccacaatggg tggaaaacgg gtgggcgtag taccggttca caatcccctc aaacccgggc
     1321 atgtccagaa agcttagccc tcgaagtcgt tcgcggggtt cttaaggatc aaatcgtggg
     1381 catggacaca aatccactgg ccgtggtggc tgcccaggca aatttgctgt tggccacaat
     1441 tcacaccctg tggcggattt gtccggcaat gcgcttgccg ctggatgcgg tactcgacca
     1501 tcccagcccc atccggtgtg gtgaccttct cgattcctca tgggagactc ccacagcgaa
     1561 tgcacgacct atgggtccag gcgcagaacg gcaaactaag ccgcagattc agccacagtt
     1621 tcatttcctg gtgggaaatc cgccgtggct tctttgggac aatctgacgg cgtcgatgag
     1681 agaacggctc gcacctttgt gtcaggaatt gggattgttt gacctatctg ggcgagaggc
     1741 acgacatggc ggggccaaac gcgacgttgg cgtggcgctc gtgtggatgg cgacacagta
     1801 tttgctgccc ggtggcaaga tgggtgtggt gcttcctgcc acactcttcc gcgggggaaa
     1861 tgctggccgc agcttcagac gattcactgt agggcctgag acgccgttgc gggtccttca
     1921 ggtagaagac ctttccggac tgccggtgtt tccgggaacc atccggagaa ccgtcctatt
     1981 ctttgcagaa aaaggtttcc ccacgcgcta tcctgttccg tattatgtct ggcataaaga
     2041 agctggacag cgagagatgg acaacggcag gctacatgct tcccgcacca aacttgtggc
     2101 agtaccctct tgccctgatg acaccgcggc cccttggtcg atcacaacag agagcgctcg
     2161 taacatttgg cagaaagtgg tggggccctc ggagtactcc gcctacctgg gtgcgaatac
     2221 cgccggggct aatggcgtgt tttggctgga aatcatcggc cacgagtgcg gacttgccgt
     2281 ggtgcgaaac tgtgctgaat ccggtcgccg agcaacgcca cacttccaag gccccttgga
     2341 gccagatttg ctctatcccc ttttgcgatg gaaagatgtg atcccggcag gggttgcctc
     2401 cacggtcatt ctgctggtac aagatcccct gactcgaaag gccattccag aggagttgct
     2461 caaagcccgc tacccgctta cttatgccta tcttagccaa tttcgagaca tcctcgaagc
     2521 tcgtgctgca cggcgaaagc ttcaccgcaa ggaaccttgg tattcgcagt acaatgtggg
     2581 tcattacacc atggccccac ataaggtcgt ttggcggaga atggattgga aatttcgcgc
     2641 cgtgttggtc ggtccgcaac cactgggaga gttaggggtc cgaccggtcg ttccccaaga
     2701 gacctgttgt ttcattccgg tggattcggt tgaggaaggg gcgtatcttc tggcgattct
     2761 taatagtcgt cttgtccggg aaattgtcca agcgtcggcc ctgcccggct cacgtggctt
     2821 tgcctcgccg cggattcttc ggacgctcgc cattccccgt tttgaccccc gagtcgatct
     2881 tcaccgccgc ctggcggaga tcggtcaaaa ggcgtcttcc accggtcaag tttcctgcga
     2941 caagtggctt gaagcaaaca atctcacgga ctgggaccgt tgcctggccg aactctacgg
     3001 cctttcgccg agtgactacg tggcattgtg cgaaaagagc gtgtgaaact acaaagggca
     3061 agttttttca cggttaggga gatgaaccat tgattccgag gcggctcagc catcgtccgc
     3121 ggcttggttt gttcttttct tcttccgggg acttggacgg tcccaaggcc gccagttttt
     3181 cttcgagttc gcgctgtttt tcctcaagga ccgctcgttc gcgcgcgatt tttgcgcgtt
     3241 ctagcgaaat ctctaactcg gccttgcgga gcttttcctt ccactcctct tccagttgcc
     3301 ggagatgctc ccgctgctcc cgcagaacct catcctgatc gaagagccgc tcgatggctg
     3361 cggcacctac tgccaccgaa ccaatgctgg tcgattgatc ctcgagtaac cgccgcaact
     3421 cggcaatctc acggtctttc tcggccagcg cggcctccgt tttcttgata acatcttgaa
     3481 tccgtatccg ttcggccgca cgttcggggt tggaatcctc ttgcgcctct gcttcgagag
     3541 ctgcgagaat acgttgtttt tcggcctccc aatcgagaat ccgtccgtcg gacgaggtca
     3601 ccaccggctt ggtccccgat tgttcgatct gcgccagttt tttctccaat tcggcgtttt
     3661 tctgcctgag ttcacggatt tcgacaatgg ccatttcgta gcgctgctga aggtccgaag
     3721 atgcctgcgg gtctgattgt ctccgtagct tttccagttc ttcttccaat tcactacacc
     3781 gaatgaccaa ggcattgaag gcagactctc gatcattcca ctgggcccgc cagcgatctt
     3841 tttcgctttc gaactccgcc cgcagttggg cctcgcgccg ctgccactgc tcggcgtccg
     3901 tttgtttttc tgattgactg atggccaact gttgctcgag gtgcgccttg atcgatctaa
     3961 gctctgccat ttcccgccgc atggcagcca attcggccgt cagttgcgca tacagttggc
     4021 tccggttctc attttccgtt cggagggatt ccccacgttc ctggatgcta cgcaccgccg
     4081 aatcaagcgc tgaaagaagc ggcaagattt tttccgatga tttcgattct ctctgagaaa
     4141 gctccgcccg caaggcctcg accgttttag ccaacccccc aagatagtcc cccaattcct
     4201 gctcggcgag gcggaacctt tcctgctggc gcgaaaggac ttcccggcag cgtgcgcggt
     4261 gctcatgcaa gagtcgaacg acttgttcac ccgccgccaa agcctcagaa gatgattcgg
     4321 aggtaatcat gagcgatttt gtctgtcttt tggctttcgt tgagtttctt cggactaggg
     4381 tcgcaccttc tggctcgatt aacggggaga acgagctttt ttccgatggg actactgccc
     4441 agctcgttat cacacagatc cctctttcat aggtctaggt tgcaggattg gacgagtcaa
     4501 attgtcatgc caattatgca gattgccaaa aacctgccga atttgccgga taaaaacaaa
     4561 aacaggggag aaacgatcgt ttctcccccc tgttgaagta tcgggaatat tcgttgcaag
     4621 tgaccgttca cggggagcgg aggattttct cgaaggatgc ccttcatgcc agcggctgtc
     4681 gtcctctgcc atctggggag ggttatcatc ctcggtgacc aaatcaccgg agggcgagga
     4741 cggcgtccct ccgtcttctt cgccaacccg aggaactcct taacgcgcaa ccccttgagt
     4801 cccaagaagt tgctaatctt ccccgtgaac ggttatcgtt gcaacgatct gagcgctact
     4861 actgagcatc ttttgtcaca cctggaaagt catccgtgcg gaaaggtgaa gccggcagat
     4921 cggccttgtt ccacacctcg atcaggcgcg gatcgtgcgg atcgacgc
//
LOCUS       DAOPTR010000132         4928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_13848_length_4928_cov_3.174225, whole genome shotgun sequence.
ACCESSION   DAOPTR010000132 DAOPTR010000000
VERSION     DAOPTR010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13848_length_4928_cov_3.174225"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            347..1483
                     /locus_tag="PKI05_03795"
     CDS             347..1483
                     /locus_tag="PKI05_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HOA51352.1"
                     /translation="MKATSVLRVRGLPMACVWAACAAATWAVFVTTLTVAEEMASREQ
                     NVPNLAQQVPYPRITLSVCYEVDPTWPQRPAELVWGQTPSVFVDNQDRVWVYTRAVPP
                     VHVYDAATGKLLFTWGEQVFGKYLEKMLAHGIKIDHRGHVWLCDVGNHAVFELDQTGK
                     ILRILGTPGEPGCDQSHFYKPTDMAITPQGHVFVTDGYGNARVVHFDDQGRYVGEWGT
                     LGVKPGEFNLPHAVVVDSKGRVYIADRNNARVQVFDQSGKLLDAWQDLVVPWGFWITA
                     DDQIWVCGCSPMPWRETDEVLSCPPKDQLFMRFDPTGRVRQLWTVPKGIDGQEQPGEL
                     NWVHGIALDSQGNIYACDIIGKRVQKFVRKSPGWSVPFVPKPKE"
     gene            complement(1603..2766)
                     /locus_tag="PKI05_03800"
     CDS             complement(1603..2766)
                     /locus_tag="PKI05_03800"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOA51353.1"
                     /translation="MPWLLTVIPCSLLISWLGGFVLRRYGPRWGLVDRPGARKVHDRP
                     MPTSGGIAIWLGLIVPLAVLQGMLWLTFDARPAPQRAPEVIAGLVARDYLPEEIVRHL
                     PGVWAQSGRLWTVLGGATLVMLLGLADDRWKLPWWLRLVAEFILATATVAAGFRLTVF
                     IDIPILTWILSALWIVALINAFNMIDNMDGLSAGVAAIACTILAAVMLLTPKELGGEP
                     QLFVAGFFLLVLGALLGFLWHNWPPARLFMGDAGSYLIGYLLAVISMAATFAGEHTPR
                     HAVLAPLCALAVPIYDLVTVVTIRLRAGASPFVGDTRHFSHRLVELGMTKAQAVLTIY
                     LMAATCGLGALLLHQVDTFGAVVILLLVGCIVSLIAILETTGRRAQRRRNNKT"
     gene            complement(2751..3311)
                     /locus_tag="PKI05_03805"
     CDS             complement(2751..3311)
                     /locus_tag="PKI05_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HOA51354.1"
                     /translation="MGVPTAPDPVLLLLAVFSRYDEAIEWARQRIVEEWGPIALTSPP
                     FPFTHTDYYTPTMGPGLRKVFFAPADLIDPGRLPAIKIQTNAWESEYAEMKRFPEPRP
                     VNIDPGYLTLGKLVLASTKDFAHRIYLGLGIYGEVTLFYRHHRWEHHEWTFADYRQPE
                     YHEFFSRCREYLHERLREVTAPCRGS"
     gene            3652..4833
                     /locus_tag="PKI05_03810"
     CDS             3652..4833
                     /locus_tag="PKI05_03810"
                     /inference="COORDINATES: protein motif:HMM:NF025182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HOA51355.1"
                     /translation="MIRQELIILCLALVVPATQRTWAIELSGVFVPKFTVEKAPELEK
                     WFDASDKWGGADGVFSIPLDEHRLLFTFGDTFTTRQRRKMVNNSIAIMQLEGRTPVDV
                     AFYFGQTTDGEIRSFAVPEDHRGLFWLAAGFRDGDRLHLFAAHIELTPMGGPFGFRQI
                     GEALLTVNNPDDPPPQWHIQQQALPYFRSDDQGTAVMGSAVCVPAEANSQYVYLYGFE
                     EEKTPLFPIKHAIVARVAKGRADHINEWEFYCGPSQLPPNAQPQDFIIRAENWTRDFS
                     RATRLAREVASEYSVSYVPCLQAYVMVYTRLGLSQEIMMRRAPTPVGPWSEALRLYRV
                     PDLPEDQKAFYYAAKAHPALAADDELVISYVANSFDFGRLVRTPEIYRPWFIRVRFIP
                     E"
BASE COUNT         1055 a         1429 c         1452 g          992 t
ORIGIN      
        1 agccctcaag gagacgttaa cccgtgtggt gggagacgta gagcgaatca aacgtgaatt
       61 ggggctgtaa gccttgcgca aacccgccgg cacctatggg cgacaccagg tttgcctctg
      121 cgacccgtgt ttcctctttc cattgggggg ccatgtccgt gtgagcggaa taattcgccg
      181 ggtcacacac cgttctggcg gggaacgact ttgggtcacc aatggggatg accgccacgg
      241 gggcgcgtga ccttttctct gctgcgcttt actttccctc attgcctcga cacaatagtg
      301 ggctgagatt tcccgcgtgt tcaaaatcct atcaggagtg acgctcatga aagcaacatc
      361 tgtcttgaga gtgcgagggt taccgatggc atgcgtgtgg gcggcttgtg ccgcggcaac
      421 ttgggcagta tttgtcacga ctcttacggt cgccgaggag atggcatcac gggaacaaaa
      481 cgttcccaac cttgcccagc aggtgcccta tccacggatc accctttccg tctgttacga
      541 ggtggatcca acctggccgc aacgtccggc cgaacttgtt tggggccaga cgccttccgt
      601 ttttgtggac aaccaggacc gtgtttgggt ttatacccgc gccgtgccgc cggtccatgt
      661 gtatgacgcc gctacgggaa agcttctctt tacctgggga gagcaggtct tcggcaagta
      721 tttggaaaag atgctggccc atggcatcaa aatcgaccac cgcggccatg tgtggctttg
      781 tgatgtgggc aatcacgccg tttttgaatt ggatcaaacc ggcaagattc ttcgcatctt
      841 ggggacgccc ggcgaacccg gctgcgatca atcccacttc tacaagccca ccgacatggc
      901 catcacgccc caaggacatg tctttgtgac ggacggttac ggcaatgccc gcgtggtcca
      961 cttcgatgat cagggacgct atgtcggcga atggggaacg ttaggcgtta aaccgggcga
     1021 gttcaatctg ccgcacgccg tcgtggtgga ttccaagggg cgtgtttata ttgccgatcg
     1081 gaataacgcg cgggtacagg tttttgacca gtctggaaaa ctccttgatg cgtggcaaga
     1141 cctcgtggtt ccctggggat tttggatcac cgccgacgat caaatctggg tctgcggttg
     1201 ttcgcctatg ccgtggcggg aaaccgacga ggttttaagc tgtccgccca aggaccaact
     1261 tttcatgcgg tttgatccca cgggtcgggt gcggcaactt tggaccgtgc cgaagggaat
     1321 tgatgggcag gaacagcccg gcgaactgaa ttgggtccac ggcatcgccc tagattcgca
     1381 agggaacatt tacgcttgcg acattattgg caaacgggtc cagaagtttg tccgcaaaag
     1441 tcccggctgg tcggtaccct tcgtgcccaa gccgaaggaa taatccaccg gcggacaatc
     1501 gtcagcgctg cgggtccgac cgcgaaagac gggaactatc cggtcggttg ggagcgattg
     1561 tggcaacgcg cttgttggcc atagtggctc gcaggatggc cgttatgtct tattgttgcg
     1621 tcgccgctgg gcgcgtcgtc ctgtggtctc caggatggca atcaaactga cgatgcagcc
     1681 caccaggagc aagatcacca cggccccaaa ggtatccacc tgatggagga ggagtgcacc
     1741 cagcccgcac gtagccgcca ttaggtagat ggtcaacacg gcctgtgcct tggtcatacc
     1801 cagctcgaca agccggtgcg agaagtgccg cgtgtcaccg acaaaagggc tggcgccggc
     1861 ccgcaggcgg atggtcacca ccgtgacgag gtcgtagatg ggcacggcca gggcacagag
     1921 gggcgccaac acggcgtggc gtggtgtgtg ttcgccggca aaggttgccg ccatcgatat
     1981 gacggccagg agataaccga tcaggtaact gccggcatcg cccatgaaaa gtcgcgcagg
     2041 gggccaattg tgccacaaaa agcccagcaa ggcccccaag accaggagaa agaaacctgc
     2101 cacgaacaac tgtggctcgc cgcctaactc tttgggcgtc agcaacatca cggcggcgag
     2161 gatcgtgcag gcaatggcgg ccacgccggc cgaaagccca tccatgttgt cgatcatatt
     2221 aaaggcgttg atgagggcca cgatccacag ggcactaagt atccaagtca aaatggggat
     2281 atcaatgaac acggtgaggc gaaagccggc cgccactgtg gccgttgcca ggatgaactc
     2341 ggctacgagc cgcagccacc aggggagttt ccaacggtcg tcagccaatc ccaggagcat
     2401 aaccagcgtg gcgccgccca agaccgtcca aagccgaccg ctttgtgccc aaacgccggg
     2461 taaatggcgg acaatctcct cgggcaagta atcgcgcgcg accagtccgg ctatgacctc
     2521 aggtgcccgc tgcggggccg ggcgggcgtc gaaggtaagc caaagcattc cctgcaacac
     2581 ggccaacggc acgatgagcc ccaaccagat ggcaattcca cccgaggtgg gcatgggccg
     2641 atcgtggacc ttccgcgccc caggccgatc gacaagcccc cacctgggac cgtaacgtcg
     2701 cagcacgaac ccgcccagcc aacttatgag gagggaacag ggaatgacag tcaggagcca
     2761 cggcacggcg cggtcacctc ccgcaaacgt tcatgaagat actcccggca gcgggagaag
     2821 aactcgtggt actctggctg ccggtaatcg gcaaacgtcc attcgtggtg ttcccagcgg
     2881 tgatggcgat aaaacagggt gacctcgcca taaatgccca ggccaaggta aatccgatgg
     2941 gcaaagtcct tggtcgaagc caggaccaat ttgcccaacg tcaaatagcc ggggtcgata
     3001 ttcaccggcc gcggttccgg aaatcgcttc atctcggcgt attcgctttc ccatgcattg
     3061 gtttgaatct tgatcgccgg caaccggccg gggtcgatca aatccgccgg cgcgaaaaag
     3121 accttccgga gaccaggccc catcgtcggc gtgtaataat ctgtgtgagt gaaggggaag
     3181 ggcggactcg tgagggcgat cggtccccat tcttcgacta tccgctggcg ggcccattcg
     3241 atggcttcgt cataccgact gaagacggcc aggagaagca acacgggatc aggggccgtt
     3301 ggaacaccca tttttacatc tcctcaaggg aaggaacaac gtactcaagg gatttcccag
     3361 cgatgtcatt tccggagacg atgactcttg cttgattcta ccatcatccc tcacactaat
     3421 gggaaatcgg tctcggccat tcagagtcta tcccaaaaag tccccctctc cctctgggag
     3481 agggttaggg tgagggcaag cgatcgtcgg agtcgattga gaacacccgc agcgattcga
     3541 accgccctca cccccggccc ctctcccgct aaacgggcga gggaagatca tgggataggc
     3601 tcttagctgt ccaatcatta cgacttgctt gcttggaaag gaatacgtcg catgatccgg
     3661 caagaactga ttatcctgtg tttggcactc gttgtacccg cgacgcaacg cacttgggcg
     3721 atagaactgt cgggcgtttt cgtgccaaaa ttcactgtgg aaaaggcccc cgagctcgaa
     3781 aagtggtttg atgccagtga caagtgggga ggggccgacg gcgtgttctc gatccctctc
     3841 gacgagcacc ggctgctctt caccttcggc gacacgttta ccacccgcca acggcgaaag
     3901 atggtcaata actcgatcgc cattatgcaa ttggaagggc gaacgcccgt tgacgtggca
     3961 ttctactttg ggcaaaccac cgatggcgag atacgctcct tcgccgttcc cgaagaccac
     4021 cgcgggctgt tctggctggc ggccggcttt cgggatgggg accggctcca cctgtttgcc
     4081 gcccatatcg agctgacgcc catgggtggg cctttcggct tccgtcagat cggggaagcc
     4141 cttctcaccg tgaacaatcc cgatgacccg ccaccgcaat ggcacatcca gcagcaagcc
     4201 ctcccgtact ttcggagcga cgatcaaggg acggcggtga tgggttcggc cgtatgtgtg
     4261 ccagcggagg ccaattccca gtacgtgtac ctttatggct tcgaagagga aaagacgccg
     4321 ctttttccca tcaaacatgc catcgttgcc cgcgttgcca aaggtcgggc agatcacatt
     4381 aacgagtggg aattctattg tggtccgagc cagctccccc caaacgcaca gccgcaagac
     4441 tttataatcc gtgccgagaa ctggacgagg gatttttctc gagcgaccag gctggccagg
     4501 gaagtggcgt cggagtattc cgtgtcgtat gttccctgcc ttcaagcgta cgtcatggtc
     4561 tatacccggc tcggcctttc gcaggagatc atgatgcgga gggcaccgac tcctgtcggt
     4621 ccatggagtg aggcccttcg gctctatcgg gtgcccgatc tgccagaaga tcaaaaggcc
     4681 ttttactatg ccgccaaggc ccatcccgcc cttgccgccg atgatgagtt ggtgatcagt
     4741 tatgttgcga attcgtttga ctttggacgt ctggtacgga cacccgagat ttaccgtccc
     4801 tggttcatcc gcgtgagatt cattcccgag taacaccatc agccccagcc ccactgtaag
     4861 gcgggagcct tttcatccat ggccgatcga cccgctgttt gcaccggcgg ctggcaacat
     4921 gttcggac
//
LOCUS       DAOPTR010000133         4917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_13898_length_4917_cov_3.977170, whole genome shotgun sequence.
ACCESSION   DAOPTR010000133 DAOPTR010000000
VERSION     DAOPTR010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13898_length_4917_cov_3.977170"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="PKI05_03815"
     CDS             <1..520
                     /locus_tag="PKI05_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51356.1"
                     /translation="SASARASTSQVERELGLAATSEDSDPLVLVLELMKESQVRLARK
                     DTGEQTRQIQSKIVGVFDALLNQAMQSAQPQPSAGTTQNERQEPEEKTQTTQAQPRQE
                     TEAGSQRPTQQSGISQRGELNEVLPKVWGELPERERNAVLQHAAEAIVPKYRALIESY
                     YRELSRARESSP"
     gene            546..1619
                     /locus_tag="PKI05_03820"
     CDS             546..1619
                     /locus_tag="PKI05_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOA51357.1"
                     /translation="MVLFPPRCPAELGHIKSGIFVPIVCAVVLWTLGFYGPKGIVSCE
                     EANLVTPEAAACIERGLQYLVSRQHVDGSFGSGAYRGNIAVTALAGMALMCEGSLPNR
                     GRFGEEVNRVLDYVLSNCHENGLILYVPSASRGPMYEHGFATLFLAECYGSSPRDDLR
                     EKLSRAVRLIVTTQNNEGGWRYLPQPLDADISVTICQIMALRAARNAGIHVPKETIDR
                     CVDYVKKCQNPDGGFRYMLIPGESAFARSAAGVVALYSAGIYEGPEIERGIEYLSRFI
                     PEPGVIRQEPYYFYGHYYCAQAMWQRGGQLWQRYYAGIRDELIAKQQPDGSWSDPISP
                     EFGTAMALLVLQIPNHYLPIFER"
     gene            1766..2962
                     /locus_tag="PKI05_03825"
     CDS             1766..2962
                     /locus_tag="PKI05_03825"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOA51358.1"
                     /translation="MAGLSKCASQELLLTTGTIDDAKLMAVQGDSVLFQTKEGERRIP
                     RDEIVCWGVLCEPRGGEWLCLHNGSLIAGKWLGLANGRLTWRNDYLGEFSVTVDQVAG
                     MIFRLPSSFIEKDLLIAQILDRTKGGYLLLANGDQLDGALLRRQDNGDLLVTTRFGER
                     TFSVEQLRGVSLRRVSAATQGPIDCAVGLKDGSRIIICKFTRGTEDPASKDLAPALGD
                     REIQGVLSNLLQNRIEDICFYQPFGNVVYLSDLAPETFIAVPFLEYVRPLKMDRNALG
                     GWLRTEEGAFLKGIGTYSACRVRYNLQGRFSRFAAEVAIDACTQGKGSVMCHVLVDGE
                     RKVSVGPIRGGDRPVPVVVDVTGAASLDLVVDFGERADEQDYVNWLNARLIPATTSLT
                     QEKHGN"
     gene            3318..3641
                     /gene="groES"
                     /locus_tag="PKI05_03830"
     CDS             3318..3641
                     /gene="groES"
                     /locus_tag="PKI05_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOA51359.1"
                     /translation="MAKKKGDKEESKLQLQPLADRVVVEREESEEKTPGGIVLPDTAK
                     KKPSRGVVVCVGPGRMLEDGTRAPMQVKVGDRVLFSAYAGDDFKIGDQELVLLREEDI
                     LAVIE"
     gene            3697..>4917
                     /gene="groL"
                     /locus_tag="PKI05_03835"
     CDS             3697..>4917
                     /gene="groL"
                     /locus_tag="PKI05_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699029.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOA51360.1"
                     /translation="MAKLIAYDQEARDALRRGVAKLAQAVKVTLGPRGRNVIIQKSFG
                     SPTVTKDGVTVAKEIDLEDVYENMGARMVREVASKTSDIAGDGTTTATVLAEAIFSEG
                     LKAVVSGINPIGLKQGIEKAVEDIVAKLKAMSIPIKTSKEMAQVAAIAANNDMAIGEL
                     LAQAMDKVGKDGVITVEESKTMETKVEFVEGMQFDRGYLSPYFVTDPQKMECVLEDCY
                     ILIHEKKISNARELVPLLEAVVNAGKPLLVIAEDVDGDALATLVINRLRGTLRCCAVK
                     APGYGDRRKAMLEDIAILTGGVALFESLGRKLEHVTLAELGRAKKVVVDKDNTTIIEG
                     AGKTEEIKGRIEQIRREIEKTTSDYDREKLEERLAKLAGGVAKLNVGAATEAEMKEKK
                     ARVEDALHATRAAVE"
BASE COUNT         1140 a         1180 c         1491 g         1106 t
ORIGIN      
        1 gtcagcttcg gcacgtgcca gcacttcaca agttgaaaga gagcttggtt tggccgcaac
       61 atctgaggat agtgatccct tggtcctggt tctggagttg atgaaagaat cccaggttcg
      121 ccttgcccgg aaagatacag gtgaacagac tcgacaaatt cagagcaaga ttgtcggtgt
      181 atttgatgcc ctgctcaacc aagcgatgca aagcgcgcaa ccacaaccat cggcaggtac
      241 aactcaaaat gagcgacaag aaccggaaga gaaaactcaa acgacccagg cgcagccacg
      301 tcaagagaca gaggctggtt cgcaacgtcc tacacagcaa agtggcattt cccaacgcgg
      361 agagttgaac gaggtacttc cgaaagtctg gggtgagctc ccggagcgcg aacgcaatgc
      421 ggtcctccaa catgcagccg aggcgattgt gcccaagtat cgtgcgttga tcgaatcgta
      481 ttatcgggaa ttgtctcgag cccgtgaatc gtctccgtaa tgaaaagggg agtggtgtga
      541 aaaggatggt gctttttccc ccacgctgtc cggcggaact cggccatatc aaatcgggca
      601 ttttcgttcc gattgtttgt gcggtggtgc tttggacgct gggattttat ggaccaaaag
      661 ggatcgtgtc ctgtgaagaa gcgaatttgg taacacccga ggctgccgca tgtattgagc
      721 ggggtctgca gtatttggtt tcccggcagc acgtggatgg ttcattcggt tcgggcgctt
      781 accgaggcaa catcgccgtg acggcgctgg ccgggatggc tctcatgtgc gaagggagtt
      841 tgcccaatcg gggtagattt ggcgaagagg tcaatcgtgt cctggattac gttttatcga
      901 attgccatga aaatggtcta atcctgtacg ttccttcggc aagccgggga cccatgtacg
      961 aacatggttt cgccacgcta tttctggcgg agtgttatgg ttcgagtccg cgagatgatt
     1021 tgagagaaaa gctcagccga gccgtgcgac tcatcgtcac tacccaaaat aacgaaggcg
     1081 gatggcgcta cttaccccag ccattggacg ccgatatttc tgtgacgatt tgccagatta
     1141 tggccttgcg ggcagcccga aatgcgggaa tccacgttcc caaagaaacg attgaccgat
     1201 gcgtcgatta tgtcaaaaaa tgccagaatc ccgatggcgg cttccgatac atgctgattc
     1261 ccggagagag cgcctttgcc cggtcagcgg ctggcgtggt ggcgctttat agcgccggga
     1321 tttacgaggg tccggaaatc gaacggggca ttgagtattt gtctcgtttc attccagagc
     1381 caggcgtgat caggcaggag ccatactatt tctatggtca ttactactgc gcgcaggcga
     1441 tgtggcagag gggtggccaa ctgtggcaac gatactatgc cggcatccgc gatgagctta
     1501 tcgcaaaaca gcagccggat ggctcgtgga gtgatcccat ctcaccggag tttggaacag
     1561 cgatggctct gcttgtgttg caaatcccta atcattacct tcccattttt gaacgatgaa
     1621 gcgtctggac gtcaaaccaa tttggtgccc tgaagaggaa cgaaaagaca gggatattgc
     1681 aggcccttgt cgtggtggcc ggcgccctcg gcatatatgg ctttttaggg ttgtggttac
     1741 cctttgggca tgtggatgtt tcggcgtggc gggtttgagt aagtgtgctt ctcaagagct
     1801 gctccttaca accgggacga tcgacgacgc aaaattgatg gctgtccagg gagacagtgt
     1861 cctttttcaa accaaagagg gcgaacggcg gatacctcgt gatgagattg tttgttgggg
     1921 agtgctgtgt gagccgagag gcggtgaatg gttgtgtttg cacaatggaa gtctgatcgc
     1981 cgggaagtgg cttggtcttg cgaatggccg gcttacgtgg cgaaatgatt accttggcga
     2041 gttttcagtt actgttgatc aagtcgccgg aatgatcttt cgcttgccgt cgtcttttat
     2101 cgagaaggac ctcctgatcg cgcagatatt ggataggaca aagggtggtt atcttctcct
     2161 ggccaatggc gatcaattgg acggcgcact cctcagacgt caggacaacg gggaccttct
     2221 ggtgaccacc cgctttggag agcgtacgtt ttcggtggaa cagcttcgcg gcgtttcatt
     2281 gcggagagta tccgcggcaa cgcaaggccc catagattgc gcggtgggac ttaaagacgg
     2341 gtcacgaatc atcatatgca aatttacgcg cgggacggag gacccggcct ctaaggattt
     2401 ggctcctgcc ttgggtgatc gtgagattca gggcgtactt tcaaaccttc tccagaaccg
     2461 tatcgaagat atctgttttt atcagccctt tggcaacgtg gtttatctgt cggatttggc
     2521 cccggagact tttatcgcag tgccattttt ggaatatgtc cggccgctaa agatggatcg
     2581 caatgccctc gggggatggt tacgaacaga ggaaggcgcc tttctgaagg gaatcgggac
     2641 ctatagtgcc tgccgggtcc ggtataacct tcagggccgg tttagccgat ttgcggcaga
     2701 ggtggccatt gatgcatgca cccagggtaa gggcagcgtc atgtgccatg tcttggtgga
     2761 tggagagcga aaagtgagcg tgggtcccat ccgggggggt gaccgccctg tgcccgtcgt
     2821 cgttgacgtc accggggcgg caagtttgga ccttgtggtg gattttggtg aacgagccga
     2881 tgaacaggat tatgtgaatt ggctcaacgc gcgacttatc ccggcaacta cgtctttaac
     2941 tcaagagaaa cacggcaatt gagtttggca atgcccgaca gttgggggct tgctacgctg
     3001 ccatgtcacg actccagccg acgtttggcc cactacggtg ccaatttggc agagacctca
     3061 gataatagcc gacgcctttg acaaaaacgc aaggtttcta ccagtcccgc cacatttcgg
     3121 tttcgaagcg ttagtttcct tagataatac acttacgtcg aagtcgcagg attatgctat
     3181 tttggcgcgc aaattgcgtc ggagctgagc cgagtcatta ttgactgggc atgcggcaat
     3241 catccaaggc gaatgtcaaa gaaaactgcc gttttttgtg atagcttgac gttaccaaca
     3301 ttggaggttt tgcacacatg gcgaagaaga aaggcgacaa agaggaatcc aagcttcaac
     3361 tgcagccgct agcagatcgg gtcgttgtgg agagggagga gtcagaagag aagacacccg
     3421 gtggcatcgt cctgcctgat accgcgaaaa agaaaccgtc acgcggtgtt gttgtctgcg
     3481 tcggtccggg tcgcatgtta gaagacggta cgcgggcgcc gatgcaggtc aaagttggtg
     3541 accgcgtcct cttcagtgct tatgcgggtg atgactttaa gattggcgat caagaactgg
     3601 tcctgctgcg ggaagaagac atcttggcgg tgatcgagtg attgaccgtt tccgccggtt
     3661 gaaattgcta ggcagacaaa gaggagtagt tgacctatgg ctaaactcat tgcttacgat
     3721 caggaagcgc gcgatgccct ccgtcggggc gttgccaaac tggcccaagc ggtaaaggtc
     3781 acactggggc cgcgaggccg aaacgtcatc atccaaaaga gctttggatc tccgacggtg
     3841 accaaagacg gtgtgacggt tgccaaggag atcgacctgg aagacgttta cgaaaacatg
     3901 ggtgcccgca tggtccgcga agtcgcctcc aagacgagtg acattgcggg cgatggcacc
     3961 accacggcaa ccgtcctagc ggaggctatt ttcagcgaag gtcttaaggc cgtggtctcg
     4021 gggatcaacc cgattggcct taagcagggt attgaaaagg ccgtcgagga catcgtcgcc
     4081 aagctcaagg ccatgtcaat cccgattaaa acgagcaaag aaatggccca ggtggccgca
     4141 attgctgcca ataacgacat ggccatcggg gagctcctcg cccaagccat ggacaaagtg
     4201 ggcaaggacg gggtcatcac ggttgaagaa agcaagacga tggagaccaa ggtcgaattt
     4261 gtcgaaggta tgcagttcga ccggggttat ctgtcgcctt atttcgtgac tgatccccag
     4321 aaaatggaat gcgttctcga ggattgctac atcctcattc acgagaagaa gatctctaac
     4381 gcccgcgagc ttgtgccact cctcgaggcc gttgtcaacg cagggaagcc acttttggtc
     4441 atcgccgaag acgttgatgg cgacgcctta gccacgctgg tcattaaccg tctgcggggt
     4501 acactccgct gctgtgcggt gaaggctccg ggctacggtg atcggcggaa ggccatgctc
     4561 gaggatattg ccattttgac cggtggcgtg gccttgttcg agagcttggg tcgcaagctt
     4621 gagcatgtca ccctggccga attgggtcgc gctaagaagg tcgtcgtcga taaggacaac
     4681 acgacgatca tcgagggcgc aggaaaaacc gaggagatta aaggccggat tgagcaaatc
     4741 cgtcgtgaaa tcgagaaaac gacgagtgat tacgaccggg agaaattgga agagcggctt
     4801 gccaagctgg caggcggcgt ggctaagctg aatgttggtg cggccacgga agccgaaatg
     4861 aaggagaaga aggcccgcgt tgaggacgcc ttgcacgcca cccgagcggc cgtcgag
//
LOCUS       DAOPTR010000134         4898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_13975_length_4898_cov_4.020235, whole genome shotgun sequence.
ACCESSION   DAOPTR010000134 DAOPTR010000000
VERSION     DAOPTR010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13975_length_4898_cov_4.020235"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..4746
                     /locus_tag="PKI05_03840"
     CDS             25..4746
                     /locus_tag="PKI05_03840"
                     /inference="COORDINATES: protein motif:HMM:NF019298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOA51361.1"
                     /translation="MEPRPNHFDKRVTIATPLQKAGAYLLKAEMKDGNTCFVIIWVAD
                     AVIAQKPLDNGTWFYVADAVTGQPIAKANVEFFGWQQVWRRPDAPRPQIEIKNFAEYT
                     DAEGQIILGPKIQPTEYQWLIIARAADGRMAFLGFTHLWFGRYYDAQYNATKVFTITD
                     RPVYRPGHKVHYKLWVREAKYDQPDTSAYANQLCVLELHSPKGDRLWTKPVTTDEYGG
                     IEGEWEIPEDATLGVYQLAVKGSRFRGGGSFRVEEYKKPEFEVTVQAPEKPIALGEKI
                     EATIQAKYYFGSPVTHAKVKYKIERTGYTESWYPPRPFDWLYGPGYWWFAYEYTWYPG
                     WHIWGCVRPRPFWIDWSPEPPELIGEQEVAIGEDGTVKVTIDTAPAKMLHPDQDHRYK
                     ITVEVTDQSRRTIVGSGEVLVTRQPFKVYLWLPRGYYRTGDVIEVHSCARTPDGRPVK
                     GQGKLSLFRITYKDAKPVETEARSWDLPTDEQGQARLQIKAAEPGQFRLVHTVTDEQG
                     HTIEGGYVFTIIGESLRATEDFRFNDLELIPDKPEYQPGEKVQLQVNANCRDAKVLLF
                     VRPANGVYQRPKLLALDGKSTIVPIEVTAKDMPNFFVEAVTVYDGKLYSEVKEIIVPP
                     EKRVLNVEVTPSKTEYLPGEKASVKIKLTDLAGQPFVGSTVVAIYDKSVEYISGGTNV
                     PEIKAFFWKWRRHHHSQTMHSLSRSSGNIPPRNQPSMEDLGIFGATVVEEVPEVLQSA
                     AFGGGGRGIVRRSAVMMGAPMEAAPRAAMAEGMAMEKAAAPAMNAAEAGADRAGPQEA
                     EALVAPTVRKEFADTALWVGALTTKPDGTAEVELAMPENLTTWRIKVWAMGHGTKVGE
                     AASDVVTRKNLIIRLQAPRFFVQTDEVVLSANVHNYLKQGKSVQVRLELEGNTLEPLD
                     ELVRTINIAANDEARVDWRVRVKAEGEAVVRMAALTDEESDAMEMRFPAYVHGMDKMV
                     PYSGWIRPDQEAVQFKIFIPAERRPESARLEVRFSPTLAGAMVDALPYLIDYPYGCTE
                     QTLNRFLPAVITQKVLIDLGLDLEDIRNKRINLNAQEIGAPEDREKQWQRYRREPVFS
                     KVELDRIVKVGVQRLTEMQLSDGGWGWFSGWGEFSSPHLTAIVVHGLQLAQENYVAIV
                     PDVLQRGVAWLERYQEEQLQLLRNADRKIKNVPWKSSADNLDAFVYMVLVDAGVRNEE
                     MKNYLYRDRLNLSVYALAMYGLALHKQQDQEKLAMVLRNINQYVEKDDENQTAWLNLG
                     RWSWWYWYGNDIEAMAYYLKLLARTDPRGELAPRLVKYLLNNRKHATYWNSTRDTALV
                     IEAFADYIRASGEAKPDMTVEVWIDGELKKAVEITPQNLFAFDNTFVLAGEALTTGEH
                     VVELRRKGQGPLYANAYVSYFTLEDFIPAAGLEIKIDRRYYRLKPVQATTQVAGALGQ
                     VIDQRVAKYQREPLQNLAELKSGDLVEIELILESKNDYEYLVFEDMKPAGFEPIDLRS
                     GYTRDAAGAYVEFRDNRVVFFCRALPRGRQSISYRMRAEIPGKFSALPTKGYGMYAPE
                     LRANSDEIKFRVVDVPIVPVQ"
BASE COUNT         1126 a         1413 c         1405 g          954 t
ORIGIN      
        1 gttgtggcca cctggactgt ccccttggaa cccaggccca accatttcga caagcgcgtc
       61 actattgcaa cgccgttgca aaaagcgggg gcctatttgc ttaaagcgga aatgaaagac
      121 ggcaacacct gctttgtgat catttgggtg gccgacgccg tcatcgccca aaagccgcta
      181 gacaacggca cgtggtttta tgtggccgat gccgtcacgg gtcaaccgat cgccaaggcc
      241 aacgtggagt ttttcggttg gcaacaggta tggcggagac ctgatgcccc ccggccgcaa
      301 attgagatca agaacttcgc ggaatacacc gatgccgaag ggcagatcat ccttggtccc
      361 aagatacagc ccacagaata ccagtggttg atcatcgccc gcgctgccga tggccggatg
      421 gctttcctgg gcttcacaca cctgtggttc ggccgatatt acgatgcaca gtacaacgcg
      481 acgaaggtct ttaccatcac cgatcggccg gtttatcgcc cgggacacaa ggtccactac
      541 aagctatggg tgcgcgaagc gaaatacgat caaccggata cctcggcgta cgccaatcaa
      601 ttgtgtgtgc tcgagttgca cagcccaaag ggcgatcgcc tgtggaccaa gcccgttacg
      661 accgacgaat acggtggtat cgagggcgaa tgggaaatcc ctgaagatgc cacgctaggt
      721 gtgtatcagc tagcggttaa gggttcgcgg ttccgcggcg gcggctcgtt ccgtgtggag
      781 gagtacaaga aacccgagtt tgaagtcact gtccaggccc cggaaaagcc catcgccttg
      841 ggtgaaaaaa tcgaggccac catccaggca aaatactact tcggttcacc agtgacacat
      901 gccaaggtca aatacaagat cgagcggact ggctataccg agagttggta tcccccacgc
      961 cccttcgatt ggctatatgg tccgggttac tggtggttcg cctatgagta cacctggtac
     1021 cccgggtggc atatttgggg atgtgttcgt cctcggcctt tctggattga ttggtctccg
     1081 gaaccgcctg agttgattgg cgaacaagag gtcgccatcg gcgaggatgg caccgtgaag
     1141 gtaacgatcg acacggcgcc ggccaaaatg ctccatcctg atcaggacca tcgttacaag
     1201 atcactgtgg aggtcaccga ccaatcgcgt cgcaccattg tcggctccgg agaggtccta
     1261 gtgacccgtc agcccttcaa ggtctatctc tggctaccac ggggctacta ccgcacgggt
     1321 gatgtgattg aggtgcattc gtgcgccagg acgcctgatg gccgacccgt gaaaggccag
     1381 ggaaagctct ccttattcag aatcacctac aaggacgcca aacctgtcga aaccgaggcc
     1441 cgaagctggg atttgccgac tgacgagcag ggccaggccc gtttgcaaat caaggccgcg
     1501 gaacccggac agtttcgact ggttcacacg gtcaccgatg aacaaggcca taccatcgag
     1561 ggcggctacg tctttacgat catcggcgaa agcctgcgag ccacggagga ctttcgcttc
     1621 aacgatctgg agctgattcc cgacaagccg gaatatcagc caggcgaaaa ggtccagttg
     1681 caggtcaatg ccaattgtcg cgacgccaaa gtcctgctct ttgtgcgacc cgccaacggg
     1741 gtctatcagc gcccgaagct gctcgctctc gacgggaaaa gcacgattgt gcccatcgag
     1801 gttacggcca aggatatgcc gaatttcttc gtggaagccg tcacggttta cgatggcaaa
     1861 ctctactcgg aggtcaagga aatcatcgtg ccaccggaaa aacgtgtcct caatgtggaa
     1921 gtcacgccgt cgaagacaga gtacctgccg ggcgaaaagg cgagcgtcaa gatcaaactg
     1981 accgatttgg cgggacaacc cttcgtcggc tccacagttg tggcaatcta cgacaagtcg
     2041 gtggaataca tttccggcgg gaccaacgtg ccagaaatca aggccttctt ctggaaatgg
     2101 cgtcggcacc accactccca gacgatgcat tcgctgtcgc gcagttccgg aaacattccg
     2161 ccgcggaatc agccaagcat ggaagactta ggcatctttg gggcaactgt ggtggaagaa
     2221 gttcccgaag tgttgcagtc tgctgccttc gggggtggtg gccgtgggat tgttcgccgt
     2281 agcgcggtca tgatgggtgc gccaatggag gcagcaccaa gagcggcgat ggcagaaggt
     2341 atggccatgg aaaaggccgc tgcgcccgct atgaatgcgg cggaagccgg agcagatcga
     2401 gcaggcccgc aagaagcaga ggctcttgtc gcccccacgg tccgtaaaga gtttgcggac
     2461 acggccctct gggtgggggc tttgacaacc aaacccgatg gcacggccga ggttgagctg
     2521 gccatgcccg aaaacctgac cacctggcgg atcaaggttt gggccatggg tcacggcacg
     2581 aaagtcggcg aggctgcctc cgacgtggtc acccgcaaga acctcatcat tcgccttcaa
     2641 gccccccgat tctttgtgca aacggatgag gttgtcctct ccgccaacgt ccataactac
     2701 ctcaagcagg gaaaatcggt tcaggttagg ctagagctag aaggcaacac cctagaaccc
     2761 ctcgacgagc ttgttcggac catcaacatc gctgccaacg atgaagcccg cgtggactgg
     2821 cgcgtccggg tcaaggccga aggcgaggcc gttgtccgta tggcggccct gaccgacgaa
     2881 gaatctgacg ccatggaaat gcgcttcccg gcgtacgtcc acggcatgga taagatggtc
     2941 ccctactccg gctggatcag accggatcag gaggctgttc aatttaagat tttcatcccg
     3001 gccgaacgcc gtccggaatc ggcacgcctt gaggtccgct tctcgcccac actggcgggg
     3061 gctatggtcg atgccttgcc ctacctaatc gactatccct acgggtgcac ggaacagacg
     3121 ctcaaccgct tcctcccggc cgtcatcaca cagaaagtcc tgatcgacct tggcttggac
     3181 ctcgaagaca tccgtaacaa acggatcaat ctcaacgccc aagaaattgg cgccccggaa
     3241 gaccgcgaga aacagtggca acgctaccgg cgggagcccg tctttagcaa ggtcgaactg
     3301 gaccgcatcg tgaaggttgg cgtccaacga ctcaccgaga tgcaactttc tgacggtggt
     3361 tggggttggt tctccggttg gggcgagttc tcttcacccc atcttacggc catcgtggtc
     3421 catggcctgc aattagcgca ggagaactat gtggcaatcg tccccgacgt gcttcagcgt
     3481 ggtgtggcct ggcttgaaag gtatcaggag gagcaactcc agttactgcg gaatgccgac
     3541 cgcaaaatta aaaacgtccc ttggaagtct agcgccgaca atctcgacgc gttcgtctat
     3601 atggtgcttg tcgatgccgg ggtgcgcaac gaggaaatga agaactacct ctaccgtgac
     3661 cggcttaatc tgtcggtcta tgccctggcc atgtacgggt tggcgctcca taagcagcag
     3721 gaccaagaga aactggcgat ggtcttgcga aatatcaacc agtacgtgga gaaggacgac
     3781 gaaaatcaga cggcctggct aaaccttggg cgatggagct ggtggtactg gtatggtaac
     3841 gatatcgagg ccatggccta ctacctgaaa ttgctcgccc gaaccgaccc ccgcggcgag
     3901 ctggccccac ggcttgtcaa atatctgctc aataaccgca agcacgccac gtactggaac
     3961 tccacccgcg acacggccct ggtcatcgag gccttcgccg attacatccg cgccagcggg
     4021 gaggccaagc ccgatatgac cgtcgaggtc tggatcgacg gagagttgaa gaaggccgtg
     4081 gagatcacac cgcagaacct cttcgccttt gacaatacct tcgttctggc cggtgaggcc
     4141 ctgaccaccg gcgaacatgt ggtggaactc cgccgcaaag gccaagggcc cctctatgcg
     4201 aatgcttacg tgagctactt cacgctggag gatttcatcc cggccgccgg tctggaaatc
     4261 aaaattgatc ggcggtacta ccgactgaag cccgttcaag cgacgaccca ggtggccggt
     4321 gctctcggac aggtcatcga ccagcgcgtg gcgaaatatc aacgggagcc gcttcaaaac
     4381 ctggccgaat tgaaaagtgg tgatctcgtg gagattgaac tcatcctcga aagcaagaac
     4441 gattatgaat acctggtctt cgaggacatg aaaccggccg gattcgaacc catcgattta
     4501 cgctccggct atactcgcga tgccgcggga gcatacgtcg aattccgcga taaccgggtt
     4561 gtcttcttct gtcgggccct ccccagggga cgacagagca tcagttaccg aatgcgggcg
     4621 gaaatccccg gtaaattctc ggccctgcca acgaagggct acggcatgta tgcccccgag
     4681 cttcgtgcca attccgacga gatcaaattc cgtgtcgtgg atgttcccat tgttccggtg
     4741 caataagcga ggaagcctgc cccccaaggc cggtcaaaga tgatcgatgg tcacagttcc
     4801 gccgggaatc ccccggcgtt ttctccatgc gcggagacat cccgaagaca attagagcct
     4861 atcccaatcc caaaaagtcc ccctctccct ctgggaga
//
LOCUS       DAOPTR010000135         4894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_13991_length_4894_cov_3.268651, whole genome shotgun sequence.
ACCESSION   DAOPTR010000135 DAOPTR010000000
VERSION     DAOPTR010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13991_length_4894_cov_3.268651"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PKI05_03845"
     CDS             complement(<1..243)
                     /locus_tag="PKI05_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51362.1"
                     /translation="MRVIFALLIVVALTCPVCAEVVVYPAPSGEELSADYRVSVAGQD
                     VAVYTARTLDPPFAGKQWDYGGPYSFASFDTDEPVEV"
     gene            complement(436..933)
                     /gene="tsaA"
                     /locus_tag="PKI05_03850"
     CDS             complement(436..933)
                     /gene="tsaA"
                     /locus_tag="PKI05_03850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOA51363.1"
                     /translation="MKSIVVLGIVLTSSMTAICLAESPTSFTVYPIGRVEKTDGRCRI
                     VLDEKYKPGLLGLEQWSHVQVLWWFDKNDTPQKRAIVQVHPRGDKNNPLTGVFACRAP
                     VRPNLIGLTLCKILRVQDNVVEIDDIDAFDGTPVLDLKPYAKGEDTADEVRVPEWAKR
                     APPRN"
     gene            1272..1856
                     /locus_tag="PKI05_03855"
     CDS             1272..1856
                     /locus_tag="PKI05_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51364.1"
                     /translation="MRDHQSEGPGKPGKRNRRRSPDTRHSRKKTDSQRRTGQSQGISV
                     RRIQDDVYELVYPRKARLLRDDIEEVYDMIAHEEWDLAVDELLWLLRECRELLEAHQL
                     LGRIALFQNKLDLARAHLGHAYELGLNATGKDFRGRLPFTQAANRPLLQATRDLVECL
                     IRLNERDLATSVAQQLLQWDPSDPLHVRDLCPPS"
     gene            complement(1963..3285)
                     /locus_tag="PKI05_03860"
     CDS             complement(1963..3285)
                     /locus_tag="PKI05_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51365.1"
                     /translation="MYVLSAGRCLLVCLSLLGTACQFFGQAENHNSVDQDQAAGAGLE
                     AQVEELLSRLDAPQRAVRDQAERDLMALGRQAIPFLQPKRMLSPEAALRLARVRTTLI
                     EQIVATDALPTRVTCKVTDLPLSEVVTKIAHQTGNAVTVSDEVAKLPVTWDCQEKTFW
                     EALDELAALVDADVSPAETGGLVILPQKNRAVQSRVAVAYCGVFRVRLRDVIKIERQH
                     NTSQVSLVRIEIAWEPRLQPAVIWWRGIHIDREQPESAAIEDKTLARREVPVSPQRCF
                     IILTLPVTLPSDLEQKPAVLRGSFEPVLPIGATDVVFDLTDLQKGPRSVILGRSEITA
                     ELQRRSANTCEVSLRVRYEEACGAFASHRGWFYSWPVYLDTDGKKALPRGVQLLRMTE
                     DGVTLLYQFGNAEVTGTRMVCRVPLAIVKPVVEFQLALSLEERNRSKD"
     gene            complement(3341..3487)
                     /locus_tag="PKI05_03865"
     CDS             complement(3341..3487)
                     /locus_tag="PKI05_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51366.1"
                     /translation="MGEDVETSYWRTGPRGNINIGIGMAIAASRVCESFDGHRPARGH
                     GRDP"
     gene            3523..4425
                     /locus_tag="PKI05_03870"
     CDS             3523..4425
                     /locus_tag="PKI05_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51367.1"
                     /translation="MTDFDNETTVEMAQVCPKMSFSEVLRALILFGLLFGMVALASIP
                     QGTLPALVLLHTDEKPSLCPAKNPDASPTVNLAANEQTPPGRFVMGSQTQAQAIQATV
                     ALAPVTQSALSHLENHPRLDDNSGPATAAPIRETLEDKRSFGMCLHHEATPSPSIQSV
                     QPLDGVTSSGFTSWDYQATSEAGGGQRAYTMVSLSATLESFGSVETMSPGPSVSLPPY
                     RGEEPGLRDWAFRFEKLGAVRYRLETWGNRGELYRFWCEMPVGQSSWTVRFFEAVASR
                     PEEAMENVLRQVEQWLQDASPVPR"
     gene            4706..>4894
                     /locus_tag="PKI05_03875"
     CDS             4706..>4894
                     /locus_tag="PKI05_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51368.1"
                     /translation="MWSLSPEEDVSRQPVADAFSAEFSDCDHQLARVEAVLFLARQPI
                     SARKIAQIAGLDSGSQVRA"
BASE COUNT         1112 a         1425 c         1276 g         1081 t
ORIGIN      
        1 cacttccacc ggctcatcgg tatcaaaact ggcaaacgag tacggtccac cgtaatccca
       61 ttgtttccca gcaaaaggcg gatccaatgt cctggcggta tagacggcga cgtcttgccc
      121 ggccactgaa acgcggtaat ctgccgagag ttcttcacca gaaggggccg gatacacaac
      181 gacctcagcg caaactggac aggtcaaggc gacgactatt agcagtgcaa aaatcactct
      241 catggacgct cctcctatag gttgcaaaca aggtacctca cggggtgcac atgccacaat
      301 acgaatctta catgaccgta gtgcttcgaa tggcgaattc tgatcgtctt tgcccgatta
      361 ccgtttccgc cccatggctt gcgtagttcc gcagggaatt cacaactcca tgcgtctttt
      421 cactctgcgg agatatcagt tacgaggtgg cgctcttttg gcccactccg gcacacggac
      481 ctcgtctgcc gtgtcttcgc cttttgcata tggtttcagg tccagaacag gcgtgccgtc
      541 aaaggcgtca atgtcatcga tttccacgac gttgtcttgg acgcgtagga ttttgcaaag
      601 tgttaaccca atcagattcg gcctaaccgg tgcacggcaa gcaaacacac cggtcagagg
      661 attgttcttg tcgccgcgag gatggacctg cacaatcgcg cgcttttgag gtgtgtcgtt
      721 cttgtcaaac caccacagga cttggacatg cgaccattgc tccagtccta gcaaaccggg
      781 cttgtatttt tcgtccagga cgattcggca tcggccatcg gtcttttcaa cgcggccaat
      841 cggatacaca gtgaaacttg ttggtgactc tgccaaacag atcgctgtca tggatgaagt
      901 aaggacaata ccgagaacga cgatagattt cacgactaac ctccctccat tccggacaaa
      961 ggcctcaaca ctgcttcgcg ttcgtgctct attgtggctt ccctctgact caaacacaag
     1021 acgcgaatac caacccctag aaaaaaggcc actttcgata ggctggtgct tctgcctcaa
     1081 atatcacggg acagactagg ttaccacgtt gctgttatac ttcaggatga tggatagaca
     1141 tacctgtctt tgcaaatgct tcggcatgac ctttatcttt agggccagat gccaaacagg
     1201 ctggctcttc catgcactag gtacatcgcc atcggcagtt tcatttcgga atcaacaggc
     1261 ggagttcgca aatgagggat caccaatcgg aaggtccagg caaacccggc aaaagaaaca
     1321 gacgccgttc cccggacact aggcactctc gcaagaaaac agattctcaa agacgaaccg
     1381 gtcaatccca aggaatcagc gttcgccgta tccaggatga tgtttacgag ttagtgtatc
     1441 cgcggaaagc ccgcctgctc cgcgatgaca tcgaggaggt gtacgacatg atcgcccacg
     1501 aagagtggga ccttgccgtg gacgagcttc tctggctctt gcgggaatgt cgcgaattac
     1561 ttgaggccca tcaacttttg gggcggattg cccttttcca aaacaaacta gatctcgcgc
     1621 gagcacacct tggccacgcc tatgagctag gcttgaacgc cacggggaaa gacttccgcg
     1681 gtcggctgcc ttttacacaa gcggccaatc gcccactgct ccaagccacg cgcgatctcg
     1741 tcgagtgcct gatccggctc aatgagcgag acctggccac ctcggttgcc caacagcttt
     1801 tacagtggga cccttccgat ccgcttcacg tccgcgatct gtgcccacca agttgagcaa
     1861 tcacaagaag cgacctgaaa agcccatgac gtgcggaagt cgttacctag cgcttagagc
     1921 ggagaagcgt ggcaagatgc aacgcagcgg aagtttcaaa atctagtctt tggagcgatt
     1981 tcgttcctct agagacaaag ccaactggaa ctccacgacc ggtttgacaa tcgccaatgg
     2041 cacacggcag accatgcgtg ttccagtgac ttcggcgttc ccaaactgat aaagcaaggt
     2101 cacgccgtct tcggtcatcc gaagaagttg aacaccccgc ggcaatgcct ttttcccgtc
     2161 agtatcaaga taaacgggcc acgagtaaaa ccacccacgg tgggaggcaa acgctccaca
     2221 ggcttcttca tatctaacgc gaaggctcac ttcgcaggta ttcgcggagc gacgttgaag
     2281 ctcggcggtg atctcggaac gtccgaggat cacgctgcga ggcccctttt gcaaatccgt
     2341 taagtcaaaa acgacgtccg tcgcgccgat gggtagcaca ggctcaaaac tgccccgcaa
     2401 cacggcaggt ttctgttcga gatcggacgg gagtgttaca ggcagcgtga ggataataaa
     2461 acatctttgc ggcgaaacgg gcacctcgcg gcgggcaaga gttttgtcct caattgccgc
     2521 gctctccggt tgttccctgt cgatgtggat accccgccac cagatcacgg ccggctggag
     2581 tcgcggctcc caagcgattt cgatccttac gagcgaaacc tgtgacgtgt tgtgctgcct
     2641 ctcaatttta ataacatccc gtaggcgaac tctgaagacg ccacagtagg caactgccac
     2701 tctcgactgt acggctcgat ttttctgggg aaggatgact agtccgccgg tttccgcggg
     2761 gctgacgtcc gcatccacca gtgccgccaa ttcgtcgagg gcctcccaga aagttttctc
     2821 ttgacaatcc cacgtgaccg gcagtttcgc cacctcgtca ctcaccgtca ccgcattacc
     2881 tgtctggtgt gcgattttgg taaccacttc acttagcggc aaatccgtca ccttgcatgt
     2941 gacccgggtt ggcagggcat ccgtcgccac gatctgctcg atcagagtcg ttcgcacgcg
     3001 ggcaagtcgc aacgcggctt ctggagataa catccttttt ggctgcaaga atgggattgc
     3061 ctgccgacca agagccatca agtctctttc cgcctggtca cggacggcgc gttgcggagc
     3121 atccaggcga ctgagaagct cttccacctg ggcctccagc ccggcacccg cagcctggtc
     3181 ctggtccacc gagttatggt tttccgcctg tccaaaaaac tgacatgctg tccccaaaag
     3241 tgacaagcaa actagaaggc accgtcccgc agaaagaaca tacatctcat cctccgcgcc
     3301 tcaccatgag gttgtggcac gcccgtttct cgacagtcgt tcagggatcg cgcccatggc
     3361 ctcgtgctgg ccgatgtcca tcgaacgact cgcagacccg tgatgcagca atggccattc
     3421 caatgccgat attgatgttg cctcgtggtc ccgtccgcca gtatgatgtt tccacatctt
     3481 cgcccattca gcgcattttt ccagagaccc ctcgaccgaa aaatgacaga ttttgataac
     3541 gaaaccaccg tcgaaatggc ccaagtgtgc cccaagatgt cgttttctga ggttctcaga
     3601 gccctcatct tgtttggttt gctctttggt atggtcgctc ttgcctcgat accccaaggc
     3661 accctaccgg ccctcgttct cttacatacg gacgaaaaac ctagcttgtg cccagcaaag
     3721 aaccctgacg cgtctcctac ggtcaatctg gcggcgaatg agcaaacccc gcctgggcga
     3781 ttcgtcatgg gtagccaaac tcaggctcaa gccatccagg ccactgtcgc cctcgcgcca
     3841 gtcacccaga gtgccctttc tcacctggag aatcacccac gcctcgatga caactccgga
     3901 ccagccacgg cagcgccaat aagggaaact ttagaggata agcggtcatt cggaatgtgc
     3961 ctccatcatg aagcgacccc gtcgccgtca atccaatccg tgcagccact cgacggcgta
     4021 acatcctctg gttttacgag ttgggactat caagccacgt ctgaagcagg tggtggccaa
     4081 cgtgcgtaca caatggttag cctgagtgcg acccttgaga gttttggcag cgtcgagaca
     4141 atgtcgcctg gcccatctgt gtctttgcca ccttacaggg gggaagagcc aggtcttcgg
     4201 gactgggcgt ttcggtttga gaaacttggc gcggttcgct atcggttgga aacctgggga
     4261 aatcgggggg aattatatcg cttctggtgc gagatgccgg tcgggcaatc gagttggacc
     4321 gtcaggttct tcgaggccgt ggcgtcccgg ccagaagagg ccatggaaaa cgtcctgcga
     4381 caagtcgaac agtggctcca ggatgcctcg ccagtccccc gctgatccct gaacgacccg
     4441 attcagggcc atcgaaaccc ttgcatcacg tccgacgtgt cccctagact ggagtttttg
     4501 cttctgcaat gccgagttat cggcagacac agcgggtggg atattgggaa gtcagtgaga
     4561 cgccatttgt caagagttgt caacaaaata gatgaactgg tggcacaatc cttggacaac
     4621 ttgttacggt ggctggcccg gtgcgatggc acagggaaac cgcttttggc gtttgcaccc
     4681 accgactacc gctgcaacga agttgttgtg gtcactctca cctgaagaag acgtttcgcg
     4741 gcaaccggtt gcggatgcat tttcagcgga gttttccgac tgtgaccacc aattagcccg
     4801 ggtggaagcc gtcctcttct tggcgcggca gccaatttcg gcaagaaaaa ttgcccaaat
     4861 tgcgggtttg gatagcggta gccaagtgcg ggcg
//
LOCUS       DAOPTR010000136         4878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_14033_length_4878_cov_3.063861, whole genome shotgun sequence.
ACCESSION   DAOPTR010000136 DAOPTR010000000
VERSION     DAOPTR010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14033_length_4878_cov_3.063861"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="PKI05_03880"
     CDS             complement(<1..1020)
                     /locus_tag="PKI05_03880"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOA51369.1"
                     /translation="MMFKIIDRYIARYFFQSFFVCFFSLMCVYIVFDFFTNIDEFMRA
                     AEDSRQLAQLFFLYYGPQTLAVLDRILGLLVLTAAMFTVAWLQRQHEMTALLAAGISR
                     ARVAVPVIVGAVALIILGVITREVIIPRMIPLLSRSNTDLLGEKARDIQARYDNSTDI
                     LLRGGAAYRKDQRIAQPNFLLPPSLDQYGGQLIAENAYYLPPTADRPGGYLMDKMIRP
                     KNLHEKPSLSWKGRPVLLTPHDHPWLKPDQCFVVSDIDFEQLTGGRVWREYSSTLTLI
                     RGLHNPSLDLGADVRVRIHGRIVQPLRDLNLLFLGLPLVVRREQRNVFVALGLGVLVV
                     SLFTAI"
     gene            complement(1097..2413)
                     /locus_tag="PKI05_03885"
     CDS             complement(1097..2413)
                     /locus_tag="PKI05_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOA51370.1"
                     /translation="MRHVTEPSASIPGLLKKLSRRAEWAGGAPIANILMARTLAQPDV
                     ISLAAGFVEHNSLPVEWVEEAFRRIWANRSHALAALQYGTTSGYLPLREAILERLRQA
                     DGLSAAEHRASVDQMVITAGSNQLLYLLAEALLDPGDIVLCGAPSYFVFLGLIQSLGA
                     RTVGVATDYQGLIPEALEDRLDELERHGELDRVKAIYVASYFDNPTGVSLAADRRAAL
                     VEIAHRWSRRHRIYIIEDWAYRDLRYEGEDIRSVWTHDVSGETVIVTGSFSKSFSPGL
                     RVGWGLLPRSLLEPVLALKGHLDFGSANFNQVVLATVLQNGWFDKHVTRLRAIYHEKV
                     ATIVSALESYLPPILDVHYEAPKGGLYVWVRLPEEIDTGVDGPLFQAAVNEGVLYVPG
                     EYCFPAEGEPVRRHYLRLSFAMPDCDRLRCGVERLSRALRAVVQCD"
     gene            complement(2624..4117)
                     /locus_tag="PKI05_03890"
     CDS             complement(2624..4117)
                     /locus_tag="PKI05_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOA51371.1"
                     /translation="MTTRVMLICFLLIGSLGLQGKACAEPARPNVLFIAIDDQNDWVG
                     CLGGHPLAKTPYLDSLAARGTVFLNAHVNSPLCNPSRTSLMLGLRPTTTGIYGLAPWF
                     RKVEALKDRVTLPQHFKAHGYRTYTAGKVYHGGVGGPAERAAEFDVWGPSPGIGVKPE
                     KKLIPPTPMGNHPLMDWGVFPHRDEDKGDYRLATWAIEQLRSMPRNQPYFLAVGFFLP
                     HVPCYVTQKWYDMFPNDDSVLPLVRDNDRDDTPRFSWYLHWYLPEPRLKWVKENNQWR
                     NLVISYLASTAFVDAQIGRILEALEETGLAKNTIVVVWGDNGFHLGEKGITGKNTLWE
                     RGTRVPLIFAGPGITPGQRCTQPVELLDIYPTLIELCGLTPRNDLEGISLVPQLRDAS
                     TKRDRPAITSHNQGNHAVRSERWRYIRYADGSEELYDMINDPHEWTNLAGRPEYASVI
                     EEHRKWLPKIDLPPAPGSADRVLTYDPATDEAVWEGMTIRRTDPIPE"
     gene            4643..>4878
                     /locus_tag="PKI05_03895"
     CDS             4643..>4878
                     /locus_tag="PKI05_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51372.1"
                     /translation="MMSCRENSGWTLARFIPKDFHRIRSLFSKGPGSLGPNDVNSKNR
                     NDDKKAKERRAPGGPAVLWYLLVGLAISFLLAGL"
BASE COUNT         1166 a         1416 c         1294 g         1002 t
ORIGIN      
        1 aatggccgtg aagaggctga ccacgagtac ccccagcccc agggccacaa acacgtttct
       61 ctgctcgcgg cgaaccacaa gcggcaaacc caagaagagg agattgaggt cgcggagcgg
      121 ttggacgatc cgtccgtgaa tgcgaacccg tacatcggcc cccaggtcca aactcggatt
      181 atgtagtccc cgaattagcg tcaaggtcga tgaatattct ctccatacgc gtcccccggt
      241 gagttgctcg aaatcaatgt cgctgacgac aaaacactga tccggcttta accaggggtg
      301 atcgtggggc gtcaaaagga cgggacgacc cttccacgaa agggaaggct tttcgtggag
      361 gtttttcggc cgaatcatct tgtccatcaa gtatccgccg gggcgatccg ctgtgggcgg
      421 taagtaatag gcattctcgg caatcaattg tcccccgtac tgatcgagac tgggaggaag
      481 gaggaagttt ggttgggcaa ttcgctggtc cttgcgatag gccgccccac cccgcaaaag
      541 gatatccgtc gagttatcgt accgtgcctg gatatcccgc gccttttctc ccaataaatc
      601 agtgttggac cgcgaaagga gggggatcat tcgggggatg atcacttccc gggtaataac
      661 gcctaagatg atgagcgcca cagcccccac gatgacgggt accgcgaccc gggcacgaga
      721 aatcccggcc gctagcaggg ccgtcatctc gtgctgccgc tgaagccatg ccaccgtgaa
      781 catggcggcc gtcaggacca gcaagcctaa aatccggtct aacacggcca gcgtctgcgg
      841 accatagtaa aggaagaaca attgcgcgag ttgcctgcta tcttctgccg cccgcatgaa
      901 ttcgtcgatg ttcgtaaaga agtcgaacac aatgtaaacg cacatcaagc tgaaaaagca
      961 gacgaagaag gattggaaga agtagcgggc aatgtagcgg tcaatgatct tgaacatcat
     1021 acaaacatat caccatccgt gacggtttgg ttaagtttcc cagattgcca aatcggggag
     1081 aatcctgtcg tggcgttcag tcacattgta ctactgcccg aagtgcccgc gatagtcgtt
     1141 ctaccccgca gcggaggcgg tcgcaatcag gcatggcgaa gctgagtcgc aagtaatggc
     1201 gacgaaccgg ctctccttcc gcgggaaaac aatactctcc cggcacgtac aagactcctt
     1261 cattgacggc cgcctgaaag agaggaccat ccaccccagt gtcgatctct tccggcaaac
     1321 ggacccaaac atacaggccg ccctttgggg cctcataatg gacgtcgagg atcggtggca
     1381 agtagctttc caaggcggag acgatcgtcg cgaccttctc atgataaatc gcccgcaatc
     1441 gtgtcacatg cttatcgaac cagccgtttt gaaggacggt agcgagcact acttgattaa
     1501 agttggccga accaaaatcg agatgccctt tcaaggccag cacgggttcg agtaatgagc
     1561 gaggaagtaa cccccaacct accctcaaac ctggcgaaaa ggatttggaa aaactccccg
     1621 tgacgatgac cgtctcgccg ctaacgtcgt gcgtccaaac ggatcgaatg tcctcacctt
     1681 catagcgcag atcacggtag gcccaatcct cgatgatgta aatgcgatga cgccgtgacc
     1741 agcgatgggc aatttcgact agggctgcgc gacgatccgc ggcaaggctc acccctgttg
     1801 gattatcgaa ataagacgcc acatagatgg ccttaacccg atcaagttcc ccatgtcgtt
     1861 ccaattcatc gagccggtct tcaagggcct cggggatcag cccctggtaa tcggtagcca
     1921 cgcccaccgt tcgtgccccc aggctctgga tcagccccag aaagacaaag taacttgggg
     1981 caccacaaag gacaatatca ccgggatcga gtaacgcttc agccagcaaa taaagaagct
     2041 gattgcttcc cgcggtgatc accatctgat ccacactggc gcggtgctcc gctgccgata
     2101 gaccatctgc ttgacgcaaa cgctcaagga ttgcctcgcg aagcgggaga tatccgctcg
     2161 tggtcccgta ttgcaaggca gccagtgcat gagagcgatt tgcccaaatt ctccgaaaag
     2221 cctcttcaac ccattccacg ggcaaggagt tgtgttcgac aaaaccggcg gcgagggaaa
     2281 tcacatccgg ctgggcgagt gtccgagcca tgaggatatt ggcaattggg gcaccacccg
     2341 cccattcggc acggcgactc aattttttca gtaaacccgg gatggaagcc gatggctctg
     2401 tgacgtgacg catgttttca aaccgtgctt catcttgaaa cccgcaacga aagagcactc
     2461 tcctcgggat ggcctagccc tcgctcgccg ctagcgttca tagtaaaccc gaagactctt
     2521 gaggatcaac ggcaattgac cgcgttcgtg acacacggga ctgcggtgtt tcctattatc
     2581 ccatggctca atcctaccga aaggcgttgc ccgcaggtca cggttattca ggaatggggt
     2641 ccgttcgccg gattgtcatg ccttcccaaa ctgcctcgtc ggtggcagga tcataagtca
     2701 gcacgcggtc agcgctaccc ggggcagggg gaaggtctat tttcggcaac cactttcggt
     2761 gctcttcgat gacggacgca tactctggcc ggcccgccag gttggtccac tcgtgaggat
     2821 cgttgatcat gtcatacaat tcttcgctcc catcggcgta gcggatatac cgccaacgtt
     2881 cactgcgaac ggcatggttt ccctggttat gactggtaat cgccgggcga tcccgcttcg
     2941 tcgaggcatc ccgcagttgc ggcaccaggc tgattccctc caaatcgttc cggggagtca
     3001 aaccacacag ctcgatgagc gtcggataaa tatcgagcaa ctctaccggt tgcgtacacc
     3061 gctggccggg cgtgatccca ggtcccgcga aaatcaaggg cacccgcgtg ccacgctccc
     3121 acagcgtatt tttacccgtg ataccctttt cccccaggtg gaacccattg tctccccaga
     3181 cgacaacgat tgtattcttc gccaggccgg tctcctcgag agcctcgaga atgcgtccga
     3241 tctgggcatc gacaaaagcc gtgcttgcca aatagctaat gacaaggttc cgccattggt
     3301 tgttctcttt gacccatttc agccgcggct cgggaagata ccaatgcaga taccacgaga
     3361 accgcggcgt atcatcccga tcattgtcac gcacaagcgg taaaacactg tcatcattgg
     3421 gaaacatgtc ataccatttc tgtgtgacat agcacggcac atggggcagg aaaaagccca
     3481 ccgccaagaa gtagggctga tttcgcggca tactccgcaa ttgttcaatg gcccatgttg
     3541 ctaaacggta gtcgcccttg tcctcatcac gatggggaaa cacaccccaa tccattaagg
     3601 gatggttacc catcggcgtc ggagggatga gcttcttctc cggcttgacc ccaatcccgg
     3661 gagaggggcc ccaaacgtcg aactccgctg cccgctcggc aggtccacca acaccaccat
     3721 gataaacctt gcctgcggtg taagtccgat aaccatgggc cttaaaatgc tgtggcagcg
     3781 tgacgcggtc tttcaaggcc tccaccttac gaaaccacgg ggcaagacca taaatccccg
     3841 tcgtcgttgg gcgtaatccc agcatcaaac tcgtgcggga agggttacaa aggggcgaat
     3901 tgacgtgggc atttagaaac accgtcccgc gggcggccaa gctgtctaag tagggtgtct
     3961 tcgccagggg atgcccaccc aaacatccta cccagtcgtt ttggtcgtca atggcaatga
     4021 acaacacatt cggccgtgcc ggttctgcac aggcctttcc ctgtaagcca agactcccga
     4081 tcaaaaggaa gcaaatgagc atcacgcggg ttgtcatgtt gacctcccca agattatcac
     4141 tatcccatgg cagtagaagg cgctatccta acttacgagc acacacctga ctcgcccgag
     4201 cgacatgtcc aaacggtccc gcgacttttc aaccacctcg cattgcacgt gcacaagggg
     4261 ttaaacaacg cgagggagcc gagcgattac ggcttaattc attcactatc ccaacaccaa
     4321 agccccagcg aacttggagc ctatcccaag tctccctctc cctttgggag agggctgggg
     4381 tgagggcaag cgatctttgg agtcgatcct caccccgatc ccactcccgc cgaaggggaa
     4441 aggggaggtt gggggacagg cttttagtat cacgtaaagg agtgtcaaac tcaagctttt
     4501 tccgagaaat tacgtcccgg atattcccga acccctgtcg cgctctaaac ttaaagaaga
     4561 gtgcagtacc gcagacaact gcccggcgcg tcgagggcgc ggtcaaacga ctggtttttc
     4621 acccgttaca ggaatggttt tcgtgatgtc ctgtcgagaa aactccggtt ggacccttgc
     4681 caggttcatt ccgaaggatt ttcaccgcat tcgaagtctt ttttccaaag gtccgggttc
     4741 cctcgggcca aacgacgtga actccaagaa tcgcaatgac gacaagaagg ccaaggaaag
     4801 gcgggcccca ggaggaccgg ctgtcctgtg gtacctcctt gtgggactgg ccattagttt
     4861 cttgttagcc ggcttgat
//
LOCUS       DAOPTR010000137         4871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_14048_length_4871_cov_3.200581, whole genome shotgun sequence.
ACCESSION   DAOPTR010000137 DAOPTR010000000
VERSION     DAOPTR010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14048_length_4871_cov_3.200581"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="PKI05_03900"
     CDS             complement(<1..1012)
                     /locus_tag="PKI05_03900"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HOA51373.1"
                     /translation="MCTLGEAYDAVSPLGKIYLPDCSYREMTEWALPTEQQRELESLR
                     HEMSGDPRWSRVRAFIRGGYWRNFRVKYPEANEMYARMMEVSRRIQNAISSAPADSTL
                     RDAQLELYRGQCNCPYWHGAFGGIYLPHLRNAVYEHLIAADNLIEQHFRSQSAWVDVR
                     AEDFDFDGQPEVRLANDHMVAYVAPFRGGQLYEWDVRSIRRNLLATMTRRPEVYHDKV
                     RAGASGHHEGCASIHDRVVFKQAGLESQIQYDRYPRKSLIDMFYSPETTLEQVRSGQA
                     VYWADLSNLQYETTIHANPDRVRVTLSREATVLDQTVRVTKAILLSEGSPILEVAYRL
                     ENL"
     gene            complement(931..1773)
                     /locus_tag="PKI05_03905"
     CDS             complement(931..1773)
                     /locus_tag="PKI05_03905"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51374.1"
                     /translation="MTSSTPRVRLVLLLHDHQPVGNFDHVFQQAYEDSYRPFLEVLKR
                     WEGIRVSLHTSGPLIEWLDSVHPGYLDEIAGLVNQGRIEIIGGPFYEPILAMIPSHDR
                     VGQIRRYTRWLENRLGAKVRGMWVPERVWEQSFTKDIVAAGIEYTVLDDYHFKAAGLR
                     KEQLYGYYLTEDEGRLLAVFPGSERLRYTIPFAEPHVTIEYLREIGEKHPNAVVVFGD
                     DGEKFGTWPDTKKHVYEDRWLNGSSRPSSVNRIGYECAPSERPMTPFRLWAKSICPIA
                     ATAK"
     gene            complement(2016..3041)
                     /gene="galT"
                     /locus_tag="PKI05_03910"
     CDS             complement(2016..3041)
                     /gene="galT"
                     /locus_tag="PKI05_03910"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HOA51375.1"
                     /translation="MFSFSSKTERNAELRQDPITGKWVIISPQRAERPQEFDTRPRRI
                     RHGPCPFCPGNEELTPPATLIKPNVSASPQEPSWKVRVVPNKYPALVDSDDCAIESGI
                     YRGRTGLGVHEVLIETPRHVQGISDLTAEEMLDVVEVLMSRLAALKSKEHLRYVMVFK
                     NVGPLAGASVEHTHSQIIATPVIPPTIEAELAGTGEFFRGHQSCIYCQIVQYEIAKAV
                     RTVQITPHFLAMCPFAARFPYETWILPRQHNARFSDLERGLWGELIQITKDLIQRIEQ
                     TIPGVGYNVILHDAPLHDDVPFYHWHIEILPSTARPAGFEWGSGVHINSVFPEFAARS
                     LREGQAS"
     gene            3379..4425
                     /locus_tag="PKI05_03915"
     CDS             3379..4425
                     /locus_tag="PKI05_03915"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOA51376.1"
                     /translation="MNSRSPFPFVLQTAIVLAASVLVGHQLFGQEEPMVPPLQIYPQP
                     YSQAAFVFVPIPMELVRFHFGGTLFRPFLYPIRSPSGRELTRMGHPHDPISHSHHNSV
                     WMSHADVNNSDFWSDRTEARIVCRNVVEYGEQPGTPGQDGSFPTAWLLADIEWVSEKG
                     TPLLKEERRITLQLFESAEKSWRNSHSALAGGYLIIIDSSFRPATESPVTFGQTPFGL
                     IGVRMTKTIGVRDGGGRILNSEGGVNEKGTFRLPARWMDYSGPVTVDQKAGITLFDHP
                     NNPGFPNPFHVRDDGWMGICLTLNGPITLEKESILKLRYGLWCHDGVPGKEEIDNVFG
                     IYSRLALPGQQRQQ"
     gene            4564..>4871
                     /locus_tag="PKI05_03920"
     CDS             4564..>4871
                     /locus_tag="PKI05_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HOA51377.1"
                     /translation="MDVRTLPDSERSPHAASEQSRDRRPKLMLFGLLFGLVSGLLVNI
                     AIEYAAQHPNETFSQWLPVTAIQWSIDNVIEPVGRVFLRLILSVVLPLVFSALVLAVV
                     "
BASE COUNT         1126 a         1317 c         1285 g         1143 t
ORIGIN      
        1 gaagattttc caggcgataa gcgacctcaa gaatgggact gccctcactc aagaggatcg
       61 ccttagttac ccgcacggtt tgatccaaaa ccgtggcctc gcggctgagc gttactcgga
      121 cacgatcagg attggcgtga atcgttgttt catattggag gttactcaaa tcggcccagt
      181 aaacagcttg gcccgacctc acctgttcca gtgtcgtctc gggactgtag aacatgtcga
      241 tgagtgactt ccgcggatag cggtcatact gaatctgtga ctccagacca gcctgtttga
      301 agacgacacg atcatggatg ctagcacacc cctcgtgatg accgctcgca cccgcgcgca
      361 ccttatcgtg atagacttcc ggacgccgcg tcatcgtggc aagcaggttt cgcctgatgg
      421 accggacatc ccattcgtag agttggccac cacggaaggg cgcaacgtag gctaccatgt
      481 ggtcattggc aaggcgaacc tcaggctgcc cgtcaaaatc aaaatcctcg gcccgcacat
      541 ccacccaggc actttgcgaa cgaaaatgct gttcgatgag attgtcagcc gcaattaggt
      601 gttcgtacac cgcgtttcgg aggtgaggca aataaatccc accgaaggcc ccgtgccaat
      661 aagggcagtt gcattggcca cgatataact ccaattgtgc gtctcgcaag gtggagtcag
      721 cgggcgccga gcttatcgca ttttggattc gccgactgac ctccatcatg cgtgcataca
      781 tttcgttcgc ttccggatat ttgacgcgga agtttcgcca atagccaccc cggatgaaag
      841 ctctcacgcg agaccagcgg ggatcaccgc tcatctcatg acgcagcgac tccagttctc
      901 tttgttgttc ggtgggcaag gcccattcgg tcatttcgcg gtagctgcaa tcgggcagat
      961 agattttgcc cagaggcgaa acggcgtcat aggcctctcc gagggtgcac actcgtagcc
     1021 aatcctgttc acggaggagg gcctcgaaga accgttgagc cagcggtctt cgtaaacatg
     1081 tttcttcgtg tccggccagg tgccgaattt ttccccatca tcaccgaaga ccaccacggc
     1141 gttggggtgc ttttcaccaa tctcccgaag gtattcaatc gtgacgtgtg gttcggcaaa
     1201 gggaatggta taacgaaggc gttcacttcc cggaaagacc gccaacaggc gaccctcgtc
     1261 ttcggtcaaa taatagccgt acagttgctc tttccgaagc ccggctgcct tgaaatggta
     1321 atcatccaaa acggtatatt cgataccggc agccacgatg tccttggtga aggattgctc
     1381 ccaaacacgt tccggcaccc acatcccacg gaccttagcc ccaaggcggt tctcgagcca
     1441 ccgggtgtag cgccggattt gtccgacgcg atcgtgagag ggaatcattg cgagaatcgg
     1501 ttcataaaac gggccaccga tgatctcaat tcgcccttgg ttaaccaagc cggcaatttc
     1561 atccagatag ccgggatgaa ccgagtcgag ccattcgatc aacggaccgc tggtgtgaag
     1621 agaaacccta atcccctccc aacgtttcag gacttccaaa aatgggcggt aactgtcttc
     1681 gtatgcctgt tgaaaaacat gatcaaaatt gcctacaggt tggtgatcat gcaacaaaag
     1741 aacaaggcgg accctaggag tggaagacgt cataggttga ttcatcggtg ctactcagat
     1801 gttactctag accctcagcg gacagacaga ccaatggacg acaactctcg agggctatgc
     1861 cggaaacgga ggtggggctg cgggacagcc atcgcgggga tcgagaggct cggtgagatc
     1921 ctcgcttctt tttatctagc cctctttagt tactgtatcg acaatcacaa cgcatatact
     1981 ccagattacc taggccagat attttgccag acccgttaac tcgcttgccc ttcgcggagg
     2041 gatcttgcag caaattcggg aaataccgag ttgatatgga caccacttcc ccattcaaaa
     2101 ccggcaggtc gcgcggtgct gggcaagatt tcgatatgcc aatgataaaa tggaacgtcg
     2161 tcatgcaggg gtgcatcatg aaggatgaca ttgtagccta ccccaggtat ggtctgttcg
     2221 atacgctgaa tgaggtcttt cgtgatctga atcagttcgc cccatagccc tcgctccaag
     2281 tctgaaaacc tcgcattgtg ctggcggggc agaatccagg tctcgtaggg aaagcgggca
     2341 gcaaaaggac acatcgcgag aaagtgcggt gtaatttgga cagtacggac ggcttttgct
     2401 atttcatact ggacgatctg gcaataaatg cagctttgat ggcccctaaa gaactcgcct
     2461 gtcccggcca attccgcttc gattgtcggg ggaatcaccg gggtcgcgat gatctggctg
     2521 tgggtatgct ccacagacgc ccccgccaag ggcccgacgt tcttgaagac catgacatac
     2581 cgtagatgct ccttggattt caatgccgct aatcgagaca ttagtacctc aacaacgtcg
     2641 agcatctctt cggcggtcaa atccgaaata ccctgcacgt ggcgaggggt ctcgataagg
     2701 acctcgtgaa ctccgagacc ggtccttccc cgataaatcc cggattcaat tgcgcaatca
     2761 tcactgtcaa ctagcgccgg atatttattg gggacgacac gaactttcca agatggctcc
     2821 tgaggagatg ccgaaacatt gggcttaatg agggtggcag ggggggtgag ctcctcgttc
     2881 ccgggacaga aagggcacgg gccatggcga atccgcctcg gccgcgtgtc gaattcttgg
     2941 ggacgttctg ctcgctgggg ggatatgatc acccatttcc cagtaatcgg atcttgcctt
     3001 agctcggcgt ttcgctcagt ttttgatgag aacgaaaaca tctttgcgtc atgcaccctg
     3061 ttgctattca cgagtcaacg gtagcaatca tgctcaaaag gatcaattga gtcgcgagaa
     3121 tctttccatt atcgcggtcc tcacgttaac gaaaaggccg gtttcttgcg gggcctgctt
     3181 agagccggta aactgggcaa acatgtttct tcactaccac ccagcgcaac cctgagactc
     3241 gccgctgaat cggtggttga acgaggcaga cagaatgggc tcgggtggaa tcggtgcgac
     3301 acctctgaca acgcgtgttg acccagatac cggcaggcgc ttcgcaattc aaacaatcag
     3361 gtgcgaggag gtttgaacat gaattctcgg tcgccgttcc ctttcgtact tcaaactgca
     3421 attgtcctcg ctgccagcgt attggtgggt catcaactct ttggccaaga ggaaccaatg
     3481 gttccgcccc tccaaattta tccccagccg tatagccagg cagcttttgt gtttgttccc
     3541 attccgatgg aactcgtgag gtttcatttt ggaggaacac ttttccgccc gttcctttat
     3601 cccattcgaa gcccctccgg ccgtgaacta acgaggatgg ggcatccgca tgatccgatt
     3661 agccacagtc accataattc ggtgtggatg tcgcatgctg atgttaataa ttctgatttt
     3721 tggtctgatc gaacagaggc ccgcatcgtt tgccgcaacg ttgttgaata tggagagcaa
     3781 cccgggactc ccggtcagga tggtagtttt ccgacagcgt ggcttttggc ggatattgaa
     3841 tgggtttcgg aaaagggtac gccgctgctt aaggaagagc gacgcatcac attgcaactt
     3901 ttcgaaagtg cggaaaaatc ctggcgaaat tcccactcag ccctcgccgg cggatatcta
     3961 atcatcattg actcgtcatt ccgccccgcg acggaaagtc ctgtcacttt tggacagacg
     4021 ccctttggct taatcggtgt ccgcatgacc aagaccattg gggttcgcga cggtggtggc
     4081 cgaatactta atagcgaggg cggcgtcaat gaaaaaggga ccttccgtct ccccgcacgt
     4141 tggatggact atagcgggcc ggtcacggtc gatcaaaagg ccggaatcac gcttttcgat
     4201 caccctaaca acccgggatt cccaaatccc tttcacgtcc gcgatgacgg ttggatgggt
     4261 atttgcttga cgctcaatgg accgatcaca ttggaaaaag aatcaattct caaactccga
     4321 tacggtcttt ggtgtcatga tggggttccc ggcaaagaag agattgacaa cgtatttggc
     4381 atatacagcc gattggctct acccggccaa cagcggcaac agtgacacag cgtatctccc
     4441 cggtttggaa cccaaaactt cgggagttgt caacgctcgt ggctagttct ccgattgtcc
     4501 gtcaagcgac cccagcagaa tagaagctat tatccttttt cgattgggca cggattgtct
     4561 cctatggatg ttcgcacatt accagactcc gaacgttctc cgcacgctgc ttccgaacag
     4621 tcgcgggacc ggcgaccgaa actaatgttg ttcggcctac tcttcggcct cgtgtcaggc
     4681 cttttagtga acatagccat cgagtatgca gctcaacatc caaacgagac gttcagccag
     4741 tggctaccgg taaccgccat tcagtggagt atcgacaatg tgatcgaacc ggtgggacgc
     4801 gtattcttgc gcttgattct aagtgttgtc ttgcccctgg ttttctcggc ccttgtcttg
     4861 gctgtggtgg a
//
LOCUS       DAOPTR010000138         4858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_14104_length_4858_cov_2.594004, whole genome shotgun sequence.
ACCESSION   DAOPTR010000138 DAOPTR010000000
VERSION     DAOPTR010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14104_length_4858_cov_2.594004"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PKI05_03925"
     CDS             <1..359
                     /locus_tag="PKI05_03925"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOA51378.1"
                     /translation="LLWEITLGFLVLVWGYFILVGLYKAATIRYRLTTYRFYHQKGLI
                     FRTIDSMEVIDIEDLALHQNLLERLVNVGRILLRTKDPSDPLLMLYGVYDPHEAFKRI
                     DKARRDEHVRRSIKIG"
     gene            455..1327
                     /locus_tag="PKI05_03930"
     CDS             455..1327
                     /locus_tag="PKI05_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOA51379.1"
                     /translation="MKRRTFLLTLVVISLSAVPVLAAPYKALIVTGQNAHDWKGTTPV
                     LKQLLEETGLFTVDVAQSPAKGEDMSSFKPEFKNYHVVVLNYQGDSWPEETKKAFVDY
                     VKNGGGVVVYHFACAAFPDWPEYNEIIGLGGWGNRNEKWGPYVRWRNGRVEFDFSPGR
                     GGGHGPMQPFAIDVRYPEHPIMKGLPPRFMHVPDELYGWLRGPAKNLTVLATAFAPKD
                     KGGSDEHEPILFTVEYGKGRVFFNALGHTANELKSVAFIVTYQRGAEWAASGKVTQPV
                     PDDFPTADEARVRP"
     gene            1524..3164
                     /locus_tag="PKI05_03935"
     CDS             1524..3164
                     /locus_tag="PKI05_03935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOA51380.1"
                     /translation="MTRRKQVFGQGQDEIARLNHLLRLDHTLLGGVLFGLEGHLVEVQ
                     ARALEVLRRSIPWREAVNISGMANLAVRESLNRISGAFAKYAIPEPQVAIQINLAPAG
                     IWKEGTWLDLPLAIIMLQASGYLPDLPETIEGEYVLVGEVGLHGEIRRVPGVLSIAEA
                     AMPGQTLVVPSGNERECALITARSDRKSCRVAAVSTLEEVIEFFQGKRKLENVLKEEI
                     RFEKVGEKPLDFGRIKGQERAKEAAVIAAAGGHNLLLIGPPGEGKSTLARAMAGIMPK
                     LTIDEKVELTRIYSAAGLLKTDGVVVSYRPFRAVHHTASKQALVGGGTDVIRPGEVTL
                     AHLGILFLDELPEFRRDSLEALREPMETGEITISRAKARGTYPARFTLVAAMNPCPCG
                     YYPSPACTCSREKVKAYRSRLSGPLLDRIDMQVEMEPLSLEEKLAKTQEGETPRLQRL
                     VQAARDRQTRRFAGTSIPYNAAIPAGRIQDFCQFSSNGFERFKQIVEENRMSTRRTDR
                     LAKVALTVADLEGADNIEAHHVDKAASFVIGDRDIHRD"
     gene            complement(3222..>4858)
                     /locus_tag="PKI05_03940"
     CDS             complement(3222..>4858)
                     /locus_tag="PKI05_03940"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOA51381.1"
                     /translation="VGFDRIFFWIRDQYVRVVHHSIRKKVPYLVGYVLILVLIVDLFL
                     RLPTGFIPQEDQGVLMAQITLPTGSTLEQTQAVADKVQQYFLEKESEGVVSCMSIVGS
                     GFSGRAQNNSMVYVMLKDWHLRERKDLRAKAIADRATAALSSIREASIYVFPPPPIRE
                     LGNATGFDFELIDMGGIGHDALMAARNQLLEMAAQHPVLQRVRPNGMEDVAQYRVDID
                     WERAGILGVPITSIHNTISAAFGGSYVNDFIQGGRVKRVYVQADAPYRMLPEDLQRLY
                     VRNQEGKMVPFDSFATTRWTSGSPRLERFNGFPAVNIWGEPAPGHSSGEAMRAMEELV
                     GKLPPGVGFDWRGISYQERMATTQAPILYAFSILVIFLCVAALYESWTIPFVNLLMLP
                     LGVFGALTATYLRGLPNDVYFQIGFLTTMGLSTKNAILIIQFVRDQLHQGRPLVEATL
                     TAARIRFRPVIMTSLAFFFGTLPLAVATGAGAGAMNAIGTAVTGGMVSATFIDLIFIP
                     MFFVLISQLFGRKRFHASESVKREGGETAADPTSGP"
BASE COUNT         1104 a         1368 c         1386 g         1000 t
ORIGIN      
        1 cccttctttg ggaaatcacc ctgggcttcc tggtcctggt gtggggctac ttcattttgg
       61 tcggcctata caaggcagcg acgattcgat atcggttaac gacctaccgg ttctatcacc
      121 agaagggcct gatctttcgc acgatcgact ccatggaagt cattgatatc gaggaccttg
      181 cgctccatca gaatctgttg gaacgcttgg tcaatgtggg ccgcattctc ctgagaacca
      241 aagacccttc tgatcccctt ctcatgctct atggggtgta tgatccgcac gaggccttca
      301 agcggatcga caaggcacgc cgcgatgaac acgtccggcg cagcatcaag attggctaag
      361 gggcttccgt cctaaaccgg tgccgcgacc ataggtacga gcgccggtga tgatcgtgtg
      421 acagacaacg cagtgacaac aaggagactg agacatgaaa cgacgaacat ttctgctgac
      481 acttgtagtt atcagtttaa gtgccgttcc cgtactggcc gcaccgtata aggccctcat
      541 tgtgacaggc caaaatgccc acgattggaa aggcacgacg cccgtcctca agcaacttct
      601 cgaagaaacg ggcctattca ccgtggatgt ggcgcaatca ccggccaagg gcgaggatat
      661 gagctcgttc aagccggagt tcaagaatta ccacgtcgtg gtgctcaact atcagggtga
      721 ttcctggccg gaagaaacca aaaaggcctt tgtggattac gtcaagaacg gtggcggagt
      781 ggtggtttat cactttgcct gtgcggcctt tcccgattgg ccagagtaca acgagatcat
      841 cggcttgggc ggctggggga atcgcaacga gaaatggggt ccctacgtcc gctggcgtaa
      901 tggccgtgtt gagtttgact tctcccctgg tcgtggcggt ggtcatgggc cgatgcaacc
      961 cttcgccatt gatgtccgct atccagaaca ccccatcatg aagggattac cgccacggtt
     1021 tatgcacgtt cccgatgaac tttacggctg gctccgcggt ccggcaaaga acttgaccgt
     1081 gctggccaca gccttcgccc ccaaggacaa gggcggttcc gatgagcacg aaccgatcct
     1141 atttacggtg gagtacggca aaggacgtgt cttcttcaat gccctgggac atacagcaaa
     1201 cgaattgaag agcgtggcct ttatcgtaac gtatcagcga ggcgcagaat gggcggcgtc
     1261 aggtaaggtc acccaacccg tgcctgacga tttccccacg gcggatgagg cccgggttcg
     1321 cccatagcgc cgacataccc tggcttttgg tttgaaaata gcgaacatag gtgggcgagg
     1381 agacaatgtt ggcctcgccc gccgttttgt gctttggggt gagaccatgg acgggggctt
     1441 atcgcacgga aatgtagttg ggtagctttt gaaaagtcgt tcgatcgatt gctccgcggt
     1501 ttttgggggt aggagaatga gccatgacgc gacggaagca agtctttggg cagggtcaag
     1561 atgagatcgc tcgccttaac catctccttc gcttggatca tacgctcctc ggtggggtgc
     1621 tttttgggct ggagggacac cttgtagagg tccaagcccg cgcccttgaa gtcctcagac
     1681 ggagtatccc ctggcgggag gccgttaaca tttcgggcat ggcaaacctt gccgtacggg
     1741 aatcgctcaa ccggatatcg ggagcgttcg ctaaatatgc aatcccggaa ccgcaggtgg
     1801 caattcagat caacctcgcg ccagccggta tatggaagga aggcacgtgg ctggatttgc
     1861 ctctggccat catcatgctc caggcatcgg gctacctgcc ggacctcccc gaaacgatcg
     1921 aaggcgaata cgtcctggtc ggagaagtgg gcttacatgg cgaaattagg cgggtgccgg
     1981 gcgtcctttc cattgctgag gcggcaatgc ccggacaaac ccttgtcgtt cccagcggaa
     2041 atgaacggga atgtgccctg atcactgcca gatctgatcg taagtcgtgc cgcgtggccg
     2101 ccgtctcaac tttggaggag gtgattgaat tcttccaagg aaagcgaaaa ctcgaaaacg
     2161 tcctcaaaga ggagattcgt ttcgagaagg taggggaaaa acccctcgat tttgggcgaa
     2221 tcaagggaca agagcgtgcc aaggaggcag ccgtcattgc cgccgccgga gggcacaatc
     2281 tcctcctcat cgggccgccc ggtgaaggca agtccacatt ggcgcgggcg atggccggaa
     2341 taatgcccaa gctaacaatc gacgaaaagg ttgagctgac gcgaatttac tcggcggccg
     2401 gccttttaaa gacagacggc gttgttgtct cctatcgtcc ctttcgggct gttcatcaca
     2461 cggcgagcaa acaggcgctg gtaggcggag gaaccgatgt catccgtcct ggagaggtca
     2521 cacttgccca cttgggcatc cttttcctcg acgaacttcc cgaatttcgc cgcgattctt
     2581 tggaagcctt gcgggaacct atggaaacgg gcgagatcac gatctctcgc gccaaggcca
     2641 ggggcaccta ccccgcccgg ttcacactgg tcgccgctat gaacccctgt ccctgtggct
     2701 attatcccag tcctgcgtgc acatgctcgc gggaaaaggt caaggcctac cggagtaggc
     2761 tcagtggtcc gctacttgac cgcatcgaca tgcaggtgga aatggagcct ttgagcctcg
     2821 aggaaaagct cgccaaaacc caagaaggag agactccccg tttgcagcga ctggttcagg
     2881 ctgcgcggga tcgccagacg cgtcgctttg cggggacgtc gatcccttat aatgcggcca
     2941 ttccggccgg acgtatccag gacttctgcc aattctcatc gaacgggttc gaacgtttca
     3001 agcagatcgt cgaagaaaac cggatgtcca cccgtcgcac cgatcggttg gccaaagtcg
     3061 ccttgaccgt ggccgacttg gaaggtgccg acaacatcga ggcccaccac gtcgataagg
     3121 ccgcgtcatt tgtcatcggc gatcgcgaca tccaccgaga ttgaccgccg tcaaatcacc
     3181 gcctcaggac ccacggccat gataagtcgc attgtcccgc ttcatggccc cgatgtgggg
     3241 tcagccgcgg tttctccgcc ttctcttttc accgattcgg aagcgtgaaa cctcttccgc
     3301 ccaaaaagtt gcgatatcag cacgaagaac atcggtatga aaatcaagtc gataaaggtg
     3361 gccgaaacca tcccgcccgt aacggccgtg ccaatggcgt tcatcgcgcc agccccggcg
     3421 cccgttgcca cagccagggg aagcgtaccg aagaagaaag ccagcgaggt cataatcacc
     3481 ggtcgaaacc ggattcgggc ggccgtcaat gtggcctcca cgaggggacg cccctgatgg
     3541 agctggtcgc ggacgaactg gatgatcaag atagcattct tcgtggaaag acccatcgtc
     3601 gtcaggaaac caatctggaa atagacgtcg ttgggcagac cgcggagata cgtcgccgtc
     3661 agtgcaccga agacacccaa gggcagcatg agcaagttga cgaaggggat cgtccagctc
     3721 tcgtaaaggg cggccacaca aaggaaaatc acgaggatag agaaggcata aagaatgggg
     3781 gcctgtgtgg tggccattcg ttcttgatag gaaatacctc gccaatcaaa gcccacccct
     3841 ggcggtagtt ttcctacgag ttcctccatg gccctcatgg cttcgcccga actgtggccg
     3901 ggggccggtt caccccagat gttcacagcg ggaaagccgt tgaatcgctc taagcgcggt
     3961 gagccgctcg tccagcgtgt ggtcgcgaag gagtcgaagg gaaccatctt cccttcttga
     4021 ttgcggacgt acagtctttg tagatcctcc gggagcatgc ggtaaggtgc atcagcttgc
     4081 acatagacac gtttgacacg tccaccttgg atgaaatcgt tgacgtacga acccccaaag
     4141 gccgccgaga tcgtgttatg aatcgaggta atgggaacgc cgagaatccc cgcccgttcc
     4201 caatcaatat cgacgcggta ctgggcaacg tcctccatgc cattaggtcg gactcgctgc
     4261 aaaacgggat gctgggcagc catttccaga agttgattgc gggcggccat gagggcgtca
     4321 tgcccaatcc cccccatgtc gatcagttca aaatcaaacc ccgtggcgtt tcccagttct
     4381 cggatcggcg gtggcggaaa gacgtagata ctagcttccc gaattgagga gagagccgcc
     4441 gtggcccgat cggcaatggc ctttgcccgc aggtccttcc gctctcggag gtgccagtcc
     4501 ttcagcatga catacaccat gctgttgttc tgggcacgcc ccgaaaaacc actccccacg
     4561 atggacatgc acgacaccac gccttctgat tctttctcca ggaagtattg ctggaccttg
     4621 tcggccacgg cctgtgtctg ctctaacgtt gatcccgtgg gcagggtaat ttgggccatc
     4681 agcacgccct ggtcctcttg gggaataaag ccggtgggca accgcaagaa tagatccacg
     4741 atcaacacca gaatcaacac ataacccacc aaatagggaa ctttcttgcg gatcgaatga
     4801 tggacgaccc tcacatactg atcccgaatc cagaagaaga tgcgatcaaa acccacga
//
LOCUS       DAOPTR010000139         4841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_14176_length_4841_cov_3.114501, whole genome shotgun sequence.
ACCESSION   DAOPTR010000139 DAOPTR010000000
VERSION     DAOPTR010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14176_length_4841_cov_3.114501"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PKI05_03945"
     CDS             complement(<1..571)
                     /locus_tag="PKI05_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51382.1"
                     /translation="MNVMYRLSFLARMCALSLVANIGLLARGEERTITLTVHPKEVLN
                     TVDVNVYGHFLEHIYHSVNGGLWGELVWNRSFEHHEGGRWQVIDQVVVQKGLATNQRL
                     VFGDPEWEDYEFTLEAQKLGGEEGFLILFRVRDETNFYWCNLGGWGNRWHQLERGRSG
                     EGRWYPVGPRVAGQIDSNRWYRIGIRCEGD"
     gene            complement(758..2701)
                     /locus_tag="PKI05_03950"
     CDS             complement(758..2701)
                     /locus_tag="PKI05_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOA51383.1"
                     /translation="MTPVDWAVIAGYFVILFGLAWWVILRGKDTADDYFLAGRNLGWF
                     IVGASIFASNIGSEHLVGLAGSGCTDGVAMAHYELHAWCLLILAWVFVPFYLRSKVFT
                     MPEFLERRFSPGARWLLSLISLVAYVLTKIAVGIFAGGIVFQTLLPEVHLNVGPIEMN
                     SFWVGSVLVILLTGLYTVLGGMRAVAYTEALQTFILVAGSALVTIYGLIALGGWGTLR
                     EVCGGEMFNLWKPLAPEGWTGTWKPVIDQEAKVMAWYFNTNYPWLGMLICAPISGLWY
                     WCTDQYIVQRCLGAPSEREARRGAIWAAVLKLLPVFIFIIPGMICLALSISGKAEALR
                     DEFFQAELEKALTQGAAEVKRIAVGRVIVEGGQQKVIAGQQVVTNGKIAVVGGTVEPV
                     EGGLAVTKGAVYVNAHATQAAFPLMVKYVLPPGFRGVVVAGLLAALMSSLAGVFNASS
                     TLFTMDFYSKLRPSASQHELVWTGRVATTTMVLIGLAWIPVIQGARGLYDYLQSVQGY
                     LAPPIFTVFFLGVFWKRLNAKGCLWALAIGFLLGVFRLAVDTPVKLFGVQYPEGSFLW
                     IVNNIFFQYFAVLIFVVSVTVMVVASYLSSPPDEQKIQGLTFGTVTEEQRRQSRATWN
                     WRDVAASGLVLAAIIAAYLYFRG"
     gene            complement(2771..4264)
                     /gene="araA"
                     /locus_tag="PKI05_03955"
     CDS             complement(2771..4264)
                     /gene="araA"
                     /locus_tag="PKI05_03955"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HOA51384.1"
                     /translation="MINLESKEVWFVTGSQHLYGPEALQKVAENAQKVVQGLNSDPVV
                     PVKILAKTVAATPDAIHRVCLEANTSPECIGLITWMHTFSPAKMWIAGLRDLSKPFLH
                     LHTQMEQDLPWATIDMDYMNLHQSAHGDREFGFLCSRMRLRRKVVVGHWKDPVVRQQI
                     GVWTRVAAAWDDLRSLRIARFGDNMREVAVTEGDKVEGQIRLGFSVNGYGLGDLVEKI
                     QQVSDFEVERLVREYDDLYDVAPELRADGSRRQDLFEAARIELGLRAFLVEGNFGAFT
                     DTFENLHGLQQLPGLAVQRLMAEGYGFGAEGDWKTAALVRALKVMSAGLRGGTSFMED
                     YTYDLKPGNMKVLGAHMLEVCPSIAAGRPSLEIHPLSIGGKRDPVRLVFDAAPGPAVN
                     VALVDMGNRFRILVNDVEVVAPEEELPRLPVARAVWIPRPDLKTSAAAWILAGGPHHT
                     AFSQAITAEYIEDLAEIAGVEFLHIGANTQLEQFKKELRWNEAAFSL"
     gene            complement(4301..4792)
                     /locus_tag="PKI05_03960"
                     /pseudo
     CDS             complement(4301..4792)
                     /locus_tag="PKI05_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
BASE COUNT         1220 a         1489 c         1135 g          997 t
ORIGIN      
        1 gatcgccttc acagcggatc ccaattcggt accaccggtt gctatcgatt tgaccagcga
       61 cccgcggccc caccgggtac catcggcctt ctccgctacg tcccctttcc aattgatgcc
      121 agcggtttcc ccatccacca agattgcacc agtaaaagtt tgtctcatcg cgaacccgga
      181 acaggatcag gaaaccctcc tcaccaccga gtttttgagc ctccagtgtg aattcatagt
      241 cctcccattc aggatctccg aaaaccagcc tttggttggt ggctaatccc ttctgaacaa
      301 ccacttgatc aatcacctgc caccgtccgc cttcatggtg ctcgaaactg cgattccata
      361 ctaactcgcc ccacagccct ccgttcacag aatgatagat gtgttccaaa aaatgcccat
      421 acacattgac gtcaaccgtg ttgagaacct ctttgggatg gacagtcaat gtgatcgttc
      481 gctcttcgcc gcgagccaaa agtccgatat tggcaaccaa cgagagcgca cacatccgag
      541 caaggaaaga caaacggtac atcacattca tgacaaacta acctccgatt tggtgcgaaa
      601 accttgttta ggctcgcctc acggtcgaac aaccccgaaa gcgtaccttc ccgaaaaatt
      661 gatcctaaaa atcgaccatt caaatatcgc gacacctgcc gcgtgccaac agcccttgtg
      721 ggcggtgtgg tgtctctcat atcaagccta ttccagctca acctcggaag tagagatagg
      781 ccgctatgat tgctgcaagc accaggccgg acgccgcaac atcacgccaa ttccaggtag
      841 cgcgcgattg acgccgttgc tcctcggtca ctgtcccgaa ggtcagtccc tggatcttct
      901 gctcatccgg gggactagac agataacttg ctacaaccat caccgtaaca ctgaccacga
      961 agatcagaac ggcaaagtat tggaagaaga tattgttgac tatccagagg aacgaaccct
     1021 cgggatattg gacaccaaaa agtttgacgg gggtgtccac cgccaaccgg aagaccccta
     1081 agaggaaccc aattgccagc gcccagagac atcccttggc attaagtcgc ttccagaata
     1141 cacctaggaa aaatacggta aaaataggcg gcgctaagta tccttggacg ctttgcaggt
     1201 aatcatacaa tccccgtgcc ccttggatga ccggaatcca cgccaagcca atcagcacca
     1261 tagtggtcgt ggcaacccga cctgtccaca caagttcatg ttgtgacgcc gatggccgca
     1321 acttactata aaagtccatg gtgaatagcg tcgaagaggc attgaagaca ccagccagcg
     1381 agctcatgag agcggcaagc agtccggcga ccacgacccc acgaaaaccc ggcggaagaa
     1441 cgtatttgac catgagtgga aacgcggcct gagtggcgtg ggcgttgaca tagacggcgc
     1501 cctttgtcac tgccagtccg ccctccaccg gttccacggt accgccaacg accgcgattt
     1561 tcccattagt caccacctgc tgaccggcga tgaccttttg ctgaccgcct tcgacaatga
     1621 cgcgacctac agcgatgcgt ttgacttctg cagccccttg ggtcagtgcc ttttccaatt
     1681 ccgcttgaaa gaactcatct ctgagagctt ctgccttccc actgatggac aatgcgagac
     1741 aaatcattcc gggtatgatg aagatgaaca ccggaagcag cttaagaaca gcggcccaga
     1801 tcgcccctcg tctcgcctct cgttcactgg gtgcccccaa gcaccgttgc acaatatact
     1861 gatcggtgca ccaataccaa agcccgctaa tcggagcaca aatcagcatc ccgagccatg
     1921 ggtagttggt gttgaaatac cacgccatga ccttggcttc ctgatcaatc actggtttcc
     1981 aggtgccggt ccatccctcc ggagccagcg gtttccaaag gttaaacatt tcgccgccgc
     2041 agacctcgcg gagagttccc cagcctccta aggcgatcag gccgtagatt gtgaccagtg
     2101 ccgaacccgc aaccagaata aatgtttgaa gggcctccgt ataagcgacc gctcgcatac
     2161 ccccgagcac tgtatataaa cccgtcagaa ggatgacgag caccgaaccg acccaaaagc
     2221 tattcatttc aattgggccg acattgaggt gtacctcagg caacagagtt tgaaacacaa
     2281 ttccaccagc gaaaatacca acggcaatct tggtcaagac gtaggccacc aaagaaatca
     2341 aagataacaa ccatcgcgcc cccgggctga aacggcgttc caaaaattcc ggcatcgtga
     2401 agacttttga ccgcaaataa aacggcacaa aaacccaggc caagatgagg agacaccacg
     2461 catgaagctc atagtgggcc atcgccacac catcggtgca gccagaccct gccagcccta
     2521 ccaagtgctc cgaaccaatg ttcgaagcaa agatcgatgc ccccacgata aaccatccca
     2581 agttccggcc agccaagaaa taatcgtcgg ccgtatcctt gcctcgcaga atgacccacc
     2641 aagctaatcc aaaaagaatg acgaaatacc cagcaattac cgcccaatca acaggtgtca
     2701 tcgcgttaac ccttcaatcc ctaggcatcc tcacgttacc gtacgttttg aaatacacca
     2761 gtgatattcc ttataacgag aaagcagctt cgttccaccg aagttctttc ttgaactgtt
     2821 cgagctgcgt attcgcaccg atgtgcaaga attcgacccc ggcgatttcg gccaggtctt
     2881 caatgtattc cgccgtaatg gcctgactga aagccgtgtg atgcggacca ccggccagga
     2941 tccatgccgc cgccgatgtc ttgagatccg gtcgaggaat ccagacggcc ctagccacag
     3001 gaagccgcgg cagctcttcc tccggtgcca ccacctctac atcgttgacg agtatgcgaa
     3061 aacgattccc catatcgacc aaagccacat tcacggctgg gccaggggcg gcgtcgaaga
     3121 cgagccgaac cggatcccgc tttccgccaa tcgatagggg atgaatttcg agggagggtc
     3181 tgcccgcggc aatactcgga cacacttcca acatgtgtgc gccgaggacc ttcatattgc
     3241 cgggcttgag gtcgtatgta tagtcttcca tgaaggacgt cccaccccgt agtcccgccg
     3301 acatgacctt caaggccctg actaaagccg cggtcttcca gtctccctcg gcaccgaaac
     3361 cgtaaccctc ggccattagc cgttggacag caaggcccgg cagttgctgc aagccatgga
     3421 ggttctcaaa tgtatccgtg aaggcaccga aattcccctc gaccaaaaag gcccttagtc
     3481 ccaattcaat gcgggcagcc tcgaaaaggt cttgcctgcg cgacccatct gcacgcagtt
     3541 ccggggcgac atcgtacagg tcgtcatatt cccgcaccag tcgctccacc tcaaaatcgc
     3601 ttacttgctg gatcttttcg accaggtcac ccagcccata accattcaca gaaaacccta
     3661 ggcgaatttg gccctccacc ttgtcgcctt cggtcacagc gacttcccgc atgttgtcac
     3721 caaatctggc aatccgcaat gatcgcaaat cgtcccacgc ggcagcgaca cgggtccata
     3781 cgccgatttg ctgccgaacg acaggatcct tccaatggcc gaccacaacc ttgcggcgca
     3841 accgcatgcg gctacataga aaaccaaatt cacgatcgcc atgggccgac tgatggagat
     3901 tcatgtaatc catgtcgatt gtcgcccagg gcaggtcctg ctccatttgc gtgtgcaaat
     3961 gcaagaaggg tttgctcaag tcccgcaggc ctgcaatcca catcttggcg ggcgaaaagg
     4021 tgtgcatcca tgtgatcaga ccgatgcatt ccggcgaggt attagcctcg agacagacgc
     4081 gatggatggc atcgggcgtt gcggcaaccg tcttcgccaa aatcttcacc ggaacgacgg
     4141 ggtcgctgtt aagcccctga acgaccttct gcgcgttttc ggcgaccttc tgaagtgctt
     4201 cagggccata aagatgctga cttcccgtga caaaccaaac ctctttactt tccaggttga
     4261 tcatgccttc caaccctacg tctctgctca gctccgcgtt ttaaatacga tggtcatcct
     4321 ggccatagta ggccttggga ccatgcttcc gttgatagtg tttttgcaga atatatccct
     4381 caatttgact gggttgcgat tccaaaagca acaaatgcca ggctaaagct gccacttctt
     4441 ccaaggccac ggcaacatcc acggcttcgt gagccgatcg gccaaatgtg aacgggccat
     4501 gtcctgccac caaaacgccc ggtatgacga gcgggtcaag ccccccgacg cgaaaccgct
     4561 cgataatgat cttgcccgtg tttagctcat aatcctcttc cacttctgtc gggcttagta
     4621 ctcgggttac ggggaccggg ccgcaaaaat agtcggcatg agttgtccca aaacaaggga
     4681 tctcttgccg ccattgcgaa aacacggtgg caaaccgact gtgggtatgg caaatacccc
     4741 gcacgtgctc aaagccccgg taaatcgcaa gatgtgtcgg cgtatcggtc gaaggacgac
     4801 gcttcccctc ctatttagtc tcatccagtc caagtacaac g
//
LOCUS       DAOPTR010000140         4825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_14234_length_4825_cov_2.873375, whole genome shotgun sequence.
ACCESSION   DAOPTR010000140 DAOPTR010000000
VERSION     DAOPTR010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14234_length_4825_cov_2.873375"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PKI05_03965"
     CDS             <1..195
                     /locus_tag="PKI05_03965"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOA51385.1"
                     /translation="EANDPRNPLGAKWLGLEGRLGIHAARDPRSFQDPEAPGAIFLSP
                     QDMDDVFDILDVGSRVVITP"
     gene            336..893
                     /gene="pyrE"
                     /locus_tag="PKI05_03970"
     CDS             336..893
                     /gene="pyrE"
                     /locus_tag="PKI05_03970"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOA51386.1"
                     /translation="MFDRERLRDLILQKALHFGQFTLASGKTSTYYLDCRKITLDPEG
                     ARLVGEGILDILLQSSFPDAVGGMAIGADPITAAVVTMSAVRGTPIRGFMVRKEPKGH
                     GTQQYIEGPVEPGDRVAILEDVLTTGGSALKAIERAVAFGLHVTQVIGIIDRLEGAGQ
                     AFAERGYPFAALFTIRDFGIEPNPA"
     gene            1078..1776
                     /locus_tag="PKI05_03975"
     CDS             1078..1776
                     /locus_tag="PKI05_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51387.1"
                     /translation="MEHSLHDQLKRFYAGPNSSIEVVIDGFRVDALSHGWLIEIQQGP
                     LVAIRKKIQTLMQRYPLLVVKPVVVEKQIVNLSRRGGPVVSRRRSPKRGQSWDIFDDL
                     VHMTKIFPHPNLRIDLALVDIEEIRYPGPRQRWGHDGYRLADRRLLRLRETMVLNTAG
                     DLRRLLPALPEPFESRDLAEILKIPRWVAQRIAYTLRESGAVQTVLRDRRGHHYRWVD
                     TPTDADDRLALLTA"
     gene            2079..3491
                     /gene="glnA"
                     /locus_tag="PKI05_03980"
     CDS             2079..3491
                     /gene="glnA"
                     /locus_tag="PKI05_03980"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOA51388.1"
                     /translation="MTPKEVLAFCRERGVKSVDLRFMDFPGLWQHLTIPVTALTEELF
                     EDGIGFDGSSIRGWQRINESDMLLIPQPETAALDPFAEIPTLMMICNIQDPVTKEDYT
                     RDPRNVARKAVNYLKSTGIADTCYVGPELEFFIFDDIRFDQSANYGFYYIDSIEGQWN
                     RGRDEKPNLGNKLRYKEGYFPVPPADQLFNIRNEMMHLMIECGLPVEAQHHEVATGGQ
                     AEIDLRFDELVTMADRVMIYKYIVKNVARKHNKVAVFMPKPLFGDNGSGMHTHLSLWK
                     KDQPLFAGSGYAGLSDVALYAIGGLLKHAPALCAFANPTTNSYKRLVPGFEAPVNLAY
                     SQRNRSAAIRIPMYSASPKAKRLEFRCPDPSCNPYLAFAAMLMAMIDGIQNKIHPGAP
                     LDKDIYDLPPEELKEIPKTPGSLEEALAALRNDHEFLLRGDVFTPDVIDTWIWYKTEN
                     EVDAMKLRPHPYEFCLYADI"
     gene            complement(4410..>4825)
                     /locus_tag="PKI05_03985"
     CDS             complement(4410..>4825)
                     /locus_tag="PKI05_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51389.1"
                     /translation="AVAGVLATAIGCSKSGAAYRTAPVKGKVIYNGQPVTSGGIRLQP
                     IAVPGASESNPGKPAGGEVRSDGTFVLSTYKDGDGAVVGRHKVSYIPFSKGAETYEDK
                     PEPSPYLGLVPKEQEVEIKPGQNELTIELVPMTQR"
BASE COUNT         1057 a         1285 c         1386 g         1097 t
ORIGIN      
        1 gaggccaacg atcctcggaa tccgttggga gcgaaatggt tgggcctgga gggacgtctc
       61 ggaatccacg ccgcccgcga tccgcgatcg ttccaagacc ccgaagcccc tggagcaatt
      121 ttcctgtctc cccaggatat ggatgatgtc ttcgacattt tggacgttgg gtctcgggtg
      181 gtcatcacac cctagcgtat gttgatgcgc gaatatgagg cgcatgtgat ttgatcagtc
      241 tttgctcagt cccggcttcg cgcgatcctg ctaacgagtc acaatcatac ggatacgcga
      301 cgtaaatatt gggaatcgtc agggggaatc tgtgcatgtt tgatcgcgag cgattgcgag
      361 accttatcct ccagaaggca ttgcactttg gccagtttac gttggcctct ggaaaaacgt
      421 ccacctatta tctcgattgc cgcaagatca cactcgatcc cgagggtgcc cgacttgtgg
      481 gcgaaggcat tcttgatatc ctgcttcaaa gttcttttcc ggatgctgtg ggcgggatgg
      541 caatcggggc ggatcccatc acggcggcag tggttaccat gtcggcggtc cgcgggactc
      601 cgattcgggg ttttatggtg cgtaaggaac cgaaaggaca cggaacccag cagtatatcg
      661 aaggaccagt tgaaccaggg gaccgcgtgg ccatcctgga ggatgtccta acgacgggag
      721 gatcggcgct gaaggcgatc gaacgggccg tcgcattcgg tttgcatgtg acacaggtga
      781 tcggtattat tgatcgcctg gagggtgcgg gtcaggcctt tgccgagcgc ggctatcctt
      841 tcgcagctct gttcacgatc cgtgactttg gtatcgagcc gaatccggct taaggccaag
      901 gctatcattt tcttctttga catgcatctg tgcgactttt ttgcaaaacg tgtcgatttt
      961 gcaagacccg cggacatcct gagttgagca gcttcgaaac cgcaccatcg gtaggttgcg
     1021 aaaagtcgaa aactgcggtt gctcaattga caaggctctt tcacggaggg acgttttatg
     1081 gaacactcat tgcatgacca gttgaagcgg ttctacgcgg ggccaaattc ctctattgag
     1141 gtggttatcg acgggttccg tgtggatgct cttagccacg gatggctcat cgagatccag
     1201 caagggccgc ttgtggcaat tcgcaaaaaa atccagacac tcatgcagag gtatccccta
     1261 ttggtcgtca agcctgtggt ggtagaaaaa cagattgtga atctatcccg gcggggtggc
     1321 ccggtcgttt cacggcggcg aagtcccaaa cgtggtcagt catgggatat cttcgacgat
     1381 ttggtccaca tgaccaagat tttcccccat cccaacctga ggatcgattt ggccctggta
     1441 gatatcgaag aaatccgcta tccgggtccg aggcagcgct ggggacacga tggttatcgg
     1501 ttggcagaca gaagactcct ccgcttgcgg gagacaatgg tcctcaatac ggccggcgat
     1561 ctgcggcgac ttcttccggc attgccagaa ccttttgagt ccagagattt ggcggaaatt
     1621 cttaagattc ctcgctgggt tgcccaacga atcgcctaca ccctccgcga atcaggggcc
     1681 gttcaaacag ttcttcgcga ccggcgtggt caccattatc gctgggttga taccccgacc
     1741 gacgccgacg atcggttggc acttttgact gcctaaaacg tcgataactc atgagatcga
     1801 tcatctgccc aggaaagagg cagggctgcg caagagttaa tcaccggttg atggtgcgaa
     1861 gaatctcatt gcgtgaatcg ttgttgggaa acgggctact cttcatcgac aaaaccggta
     1921 aaaaagacag tatgtgggcc gagttctggc attggcacgg tgtttgcttg aagaacgcgg
     1981 ccattgcaac gtggtgaatg tgttgctcgg cggagagcgg cgcggagaca cgctgcaatg
     2041 agcaaatttc ctggtaggca tgacgacggg agtgacacat gacccccaaa gaagttttgg
     2101 cgttttgtcg agagcgcggt gttaagtcgg ttgacctgcg gtttatggat tttccaggtc
     2161 tctggcagca tctgaccatc cccgtcacag cgctgacgga agaactgttt gaagatggaa
     2221 ttggctttga cgggtcaagt atccgaggat ggcaacggat caacgagagc gacatgcttc
     2281 tcattccgca gccggagacg gctgccctgg atccctttgc ggaaatcccg accctaatga
     2341 tgatctgtaa catccaagac cccgttacaa aagaggacta tacacgtgac ccgcgcaacg
     2401 ttgcccgaaa ggccgtcaac tatctcaaga gcacgggcat tgcggacacc tgctacgttg
     2461 ggccggaatt ggagttcttc atcttcgatg atatccggtt cgaccaatcg gcgaattatg
     2521 gcttctacta tatcgatagc atcgagggac agtggaatcg gggacgcgat gagaagccca
     2581 acttgggaaa caagctccga tataaggagg gctatttccc ggtgccgccc gccgatcagt
     2641 tgttcaatat ccggaatgaa atgatgcatt tgatgatcga gtgcgggctg ccggtcgagg
     2701 cccagcacca cgaggtggct accggcgggc aagccgagat cgacctccgc tttgacgagt
     2761 tagtcacgat ggccgaccgc gtcatgattt acaagtacat cgtgaaaaac gtcgcccgta
     2821 agcataacaa ggtggccgtg tttatgccga aaccgctgtt tggcgacaat ggctcgggaa
     2881 tgcataccca tttgtcgctg tggaaaaaag atcagcccct ttttgctggg agtggctacg
     2941 ccggtctttc ggacgtggct ctgtatgcca tcggcggcct gctgaagcat gcaccagccc
     3001 tttgcgcgtt cgcaaaccca acgacgaaca gctacaaacg actggtgccg ggattcgagg
     3061 ctcctgtcaa tctcgcgtac tcacagcgga atcggtcggc agccatccgc atccccatgt
     3121 acagtgccag ccctaaggcg aaacggttgg agttccgctg tccggatccc tcctgtaatc
     3181 cttatctggc cttcgcagcc atgctgatgg ccatgatcga tgggatccag aacaagattc
     3241 atccgggcgc accgctcgat aaggatattt acgacctgcc acctgaggaa cttaaggaaa
     3301 tcccaaagac gccgggttcg ctggaagagg ccttggcggc tttgcggaac gaccatgagt
     3361 ttctcctgcg gggtgatgtc tttacgccag atgtcattga cacgtggatc tggtacaaaa
     3421 cggaaaacga ggtcgatgcc atgaagctgc gtccccatcc ctacgagttc tgtctttatg
     3481 ccgacatctg acacgtagga tgggaaaatg aatttagagc cgatcccaca acctccccct
     3541 ttcccgttca gcgggagagg ggccgggggt gagggtggct cggattgccg ccggttttct
     3601 cgatcgactc cgacgatcgc ttgccctcac ccaaccctct cccagaggga gagggagcct
     3661 tgagatagac cttaggagag ggaatcgcat ttgcgaaaag atgaggtata atacgtataa
     3721 gggtgatgga acatccatcg caccacccct ctccgcccaa gcaagccggt ccaaagaatc
     3781 aagtgcctcc ccggattctt ttggaccggc tgctcttttt tggcaatctg gttgtcatta
     3841 caccatcgct cgaagctggc tgtccgatga tacagtgggt gcttgcgatt cggttgcgtt
     3901 caacgattgg aatctacggg gtagcctccg gcgatttgct cagagatctg ttaagaaagt
     3961 ccaggccacc gatcacggca ggcgtggcac gttggccgaa aaacgcctgt gattgaatag
     4021 tcattgctgc ccgtgacctg ggagggtcgc cgtccccggc gaccgaattt ataagaggga
     4081 ggccgtgctc ggtgaccaaa tcattggacg gcggggacgc cgtccctccc gtaggaggct
     4141 tgccatcctc agggaccgaa tggtcggacg gcggagacgc cgtacctccc ctgggagcgt
     4201 cgccgtcctc ggcgaccaga ccatctcgtt ggtcccgaga agttgcagat gtaccgcgga
     4261 gaccgtggca gtaataaaga ataagccgcg atccacgtcg cggcggtcag gtgggctttc
     4321 ttgcgagtca caggcgaacc gtcagtgagc gataggatag ctttgacggg tcaatcgtct
     4381 cgtgaaaaac gttatttgct taacacattt caacgttgcg tcatcggcac cagctcaatg
     4441 gtgagctcat tttggcctgg tttgatttca acctcttgtt ctttggggac caaaccaagg
     4501 taaggggacg gctccggctt gtcttcgtac gtctccgcgc ctttggagaa cgggatgtag
     4561 gagactttat gcctgccgac gacggccccg tcgccatcct tgtaagttga caagacgaac
     4621 gttccgtcac tccgcacctc tccaccggcc ggtttgccgg ggttactttc cgaagcacct
     4681 gggacggcga tgggctgcag acgaatcccg ccactggtca ccggctgacc gttataaatg
     4741 actttccctt tgacgggagc tgtcctgtag gctgccccgc tcttggaaca accgattgcc
     4801 gtagcaagca ctcccgccac agcca
//
LOCUS       DAOPTR010000141         4811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_14293_length_4811_cov_3.679563, whole genome shotgun sequence.
ACCESSION   DAOPTR010000141 DAOPTR010000000
VERSION     DAOPTR010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14293_length_4811_cov_3.679563"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1440)
                     /locus_tag="PKI05_03990"
     CDS             complement(103..1440)
                     /locus_tag="PKI05_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOA51390.1"
                     /translation="MSEADSDHTRSPAALKVRSFPHKPGVYLMKDEAGRVIYVGKAKD
                     LRHRAGSYFLKAAAEDPRTARLVREIRDIDYIEAESEVDAVLMEARLIKDIQPKFNKE
                     LRDDKTFPYLEIYIREDFPRVVFTREPHERGTKLYGPFTSPRALRGALQVLQKIFRFR
                     TCSLDIEENDPKWKWFRPCLLASINQCSAPCNLRISKEEYRKDIRRLQRFLEGDKEQL
                     LVDLRREMKEAAQALRFEEAARLRDEIEYLEKLDERGKIDVHVQPEAFHIDPQKGLAG
                     LQRILKLPTLPRAIEGVDIAHLAGSETVASLVRFIDGLPFKPGYKRYRIRTVEGVDDY
                     AAIREVVYRRFRRLREEDESPPDILLIDGGKGQLHAALSALEEWDGARPIILSLAKRE
                     EELYLPDRDEPLRLSRHSFALRLLQYVRDEAHRFAQHYHHLLRRKSTLGDEEA"
     gene            1728..2042
                     /locus_tag="PKI05_03995"
     CDS             1728..2042
                     /locus_tag="PKI05_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51391.1"
                     /translation="MARRTLDRKALRQQTEAAEALQGETSSEKAPARTRRSKPKVKKQ
                     TKLVALWGVFNQALVEVQTFDYSRRLDAEKLAKELSDSKKVPHFVQLVKRQVEVEIPS
                     EE"
     gene            2199..3221
                     /locus_tag="PKI05_04000"
     CDS             2199..3221
                     /locus_tag="PKI05_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51392.1"
                     /translation="MTRERTTGLKALGCLVALFVAQILGVTTGCQKSATQGGSKGPDV
                     PRLVSGRLPELGDYLPPLDEGRLEVAPPKEWIVSPRSEKYIVRFQADSGSAYPTVLVM
                     GSNSERFKTVTPENLEAFAKSVAAELESAGTKTTVKMGRIGDLIGVTYTRRAKVKDNF
                     GGIVERLFFDTVVAGRRYQFELRSPPEMVGLAEPYFYAVVSGAKVHAVGELESEEELE
                     SGEVTETPPKAEEQPAGQAQQKPAEKEPPVAEQAKVEAPKPEAKPAQKPTSQKTLPPE
                     KEKGQAKPAEQAPAEKPKEEAKKPEEPPAESQSAKEASQESKPEEKPKGKETGDLLKD
                     LDALLQ"
     gene            3353..>4811
                     /locus_tag="PKI05_04005"
     CDS             3353..>4811
                     /locus_tag="PKI05_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOA51393.1"
                     /translation="MGERTIGRLEVFFEHIFLSGRRTMHFEPGHTVGIDLGTTFSAIA
                     ILDDEGKPRVIPNEDGDIETASVILLADSGHVIVGPNRMRAAMEDPQNVIERIKRYMG
                     MPDYRRTFDGREISPEFISALILKKLRQDAEKQIGKIGNAVITVPYYFNDARRKATQD
                     AGRITGLNVVDIMNEPTAATLTYAYREGILGNPEVRMDKPKKVLVYDLGGGTFDVTVV
                     EYTPTQFRVLATDGDVQLGGVDWNDRLLDYVADTFKQRHGIDPRESPQMIQILRNDCD
                     LAKIELSEKTETVIHCRYEGRAVDVPITRDLFDSLTADLLQRTADTTLLVLEEAKVRP
                     EELEAIVLVGGSTLMPQVPQMIRRVLNREPYRGISPHTAVAEGAAIHAAILEAKFRGE
                     QSELAERIRRRLQLVQQENVNSHGLGIVALDPKTGKTVNHIMIPRNSRIPIEVRRIFK
                     TTKDNQERITVQVLEGDAPDPFACSLLGKCRITDLP"
BASE COUNT         1156 a         1325 c         1367 g          963 t
ORIGIN      
        1 cctttcgccg gaaatgtggg agcaaacccg cgagtggttt gccattcacc tcaagggtaa
       61 gcgttccgcc tgactgcgga ggcccttcaa gaagtctcgc aattatgctt cctcatcacc
      121 caatgtcgat ttgcggcgca ataagtgatg atagtgttgg gcaaatcgat gggcttcatc
      181 ccgcacatac tggagcagcc gcagcgcgaa cgaatgacgg ctcaaccgga ggggttcatc
      241 ccgatcggga agataaagct cctcctcccg cttggccaag gaaagaatga taggccgggc
      301 gccatcccac tcttccaggg ccgaaagggc cgcgtgaagt tgacccttgc cgccgtcgat
      361 aagcaaaata tcgggcggcg attcgtcctc ttcgcggagt cggcgaaacc ggcgatagac
      421 gacttctcgg atggccgcat agtcatcaac gccttccacc gtgcggatcc gatagcgttt
      481 atagcctggc ttgaacggca agccatcgat gaagcgaacc agactggcca ccgtttcact
      541 acctgccagg tgagcaatat caacgccctc aatggcccgg ggtaatgtag ggagcttaag
      601 aatcctttgc agcccggcta atcctttttg gggatcaata tgaaacgcct ccggctgaac
      661 gtggacatca atcttgccac gctcgtccaa cttctcaagg tattcgattt cgtcccgcag
      721 acgggctgcc tcttcaaatc gcagcgcctg cgccgcctcc ttcatttccc ggcgcagatc
      781 gacgaggagc tgttccttat caccttccaa aaatcgctgg aggcgacgga tgtccttacg
      841 gtattcctcc ttggaaatcc gcaggttgca cggggccgag cattgattga tcgaagccaa
      901 aagacaggga cgaaaccact tccacttggg gtcgttctct tcgatatcga gtgagcaggt
      961 ccgaaatcgg aaaatctttt gcaggacctg cagggcgcca cggagggccc ttgggctggt
     1021 gaacggccca taaagtttcg tgccacgttc atggggttcc cgcgtgaaca caacccgggg
     1081 gaaatcctct cgaatgtaga tttccagata ggggaaggtc ttgtcgtccc taagttcctt
     1141 attgaacttc ggctgaatgt ctttgatcag ccgtgcttcc atcagcacgg cgtcaacctc
     1201 ggactcagcc tcgatataat cgatatcgcg aatctcgcgg acaagccggg cagttcgagg
     1261 gtcctctgcc gctgctttca ggaagtagct acccgcccgg tgacgcaaat ccttggcctt
     1321 gccgacgtaa ataacgcggc cggcctcgtc cttcatcaaa tagaccccag gcttgtgggg
     1381 aaaactccga accttaagag cagccggcga acgcgtatga tccgaatccg cttccgacat
     1441 accgaaactc cgcaagacca tccttcacat gattatacga cttgccgcaa aagaagggtt
     1501 tttcggggag agtgacgccc acatccgggt gccatagcat cagtcatact ggaaggcgct
     1561 accccctcat cctcgtggta taagttacca cctaatctgt atgcgctagc gaaaaaccat
     1621 gtcaacaatt atgatagaga attggaactg gtaatggcga agggcaaacc gcgtggcggg
     1681 tccccttccc ggcaggtctt ggcccccatt tttcttaggt gatggcaatg gctcggagaa
     1741 cacttgaccg gaaggccttg cggcagcaga cagaggctgc ggaagccttg caaggtgaga
     1801 caagctcgga aaaggcacct gccaggactc gacgctccaa accaaaagtc aagaaacaga
     1861 ccaagttggt ggccctgtgg ggtgtcttca atcaggcgtt ggtggaagtc cagaccttcg
     1921 actactcgcg gcgacttgat gccgaaaaac tggccaagga actttcagac tccaagaaag
     1981 tgccgcattt tgttcaactc gtcaagcgcc aggtcgaggt ggaaatcccc agcgaagaat
     2041 gatggactgc tggcctggct ggaatcatgc ggcgagtgtt gcccgctatc ggcgagccgg
     2101 gggttagtgt ccttctgcat tttctcaccg ttggaaagcg ttaataaaga tggaaaagct
     2161 aaggggtggc ggaaggtagg attgacggaa ccgttgcgat gactcgagag agaacaaccg
     2221 gcttgaaagc gctcggctgc ttggttgcgt tattcgtggc tcagatccta ggtgtcacca
     2281 ccggatgcca gaagtctgcg acgcaaggcg gctcgaaagg ccccgacgtg ccgcgtcttg
     2341 tctctgggcg tttgccggaa ctgggggact atctgccacc actcgatgag ggacggctgg
     2401 aggtagcccc gccaaaggaa tggatcgtct cgccgcgttc ggaaaaatac atcgtccgtt
     2461 tccaagccga tagcggtagt gcttatccca ccgtgttggt catgggaagc aacagtgagc
     2521 gattcaagac agttacgccc gagaacctgg aggcgtttgc caagtctgtt gctgccgaat
     2581 tggaatcggc tggcaccaag acgacggtca aaatgggccg gattggagac ctcatcggcg
     2641 ttacctacac ccgccgggcg aaggtcaagg ataatttcgg cggaatcgtg gaacggctct
     2701 ttttcgatac ggtcgtggcg ggtcggagat accagtttga gcttagatct cctcccgaaa
     2761 tggttgggct tgctgagccg tacttttacg ccgtcgtcag cggtgcgaag gtccacgccg
     2821 ttggagaatt ggagagcgaa gaagagctcg agagcgggga agtgaccgag actccgccaa
     2881 aggcagaaga acaacctgcc ggccaggctc agcaaaagcc cgccgagaag gaaccgccgg
     2941 ttgcggaaca ggcaaaggtt gaagctccca agcccgaggc caagccggcc caaaagccga
     3001 ccagccaaaa gacgcttccg ccagaaaaag aaaagggcca ggccaagccg gcggagcagg
     3061 cgccagcaga aaaacccaag gaagaagcta aaaaaccgga agagcctccg gcggagtccc
     3121 aatcggcaaa ggaggcctct caggaaagta aacccgagga aaaacccaag gggaaggaaa
     3181 cgggagattt actcaaggat ctcgacgcac tcttgcagta atcccaggaa gggcgccaag
     3241 tcagtcgcgg ccgacaaaat tttgcccgga tatcgacatc gcgtccgcct ctgatcacaa
     3301 tgaaaagcaa gtttgctttg ttagaaattg ccgtttgcca gggatgcggt tattgggcga
     3361 aaggacaatc ggtcgtcttg aggtattctt tgagcacatc tttttgtcgg gaaggcgaac
     3421 gatgcatttc gaacctggtc acaccgttgg aatcgatctc gggaccacgt tttccgcgat
     3481 cgcaattctt gatgatgaag gaaaaccgcg ggtcattccc aacgaagatg gagacatcga
     3541 aacagcgagt gtgattcttc tcgccgatag cggacacgtg atcgttggtc ccaatcggat
     3601 gcgggcagct atggaagacc cccaaaacgt cattgagcgc atcaagcgct acatggggat
     3661 gcccgactat cgaaggacgt ttgatggccg tgaaatttcg ccagagttca tttcggccct
     3721 catccttaag aagcttcgcc aagacgcgga aaagcagatc ggcaagatcg gcaatgccgt
     3781 gatcacggtt ccttactatt tcaacgacgc cagacggaag gccactcagg atgcgggccg
     3841 catcaccggc ttaaacgtcg ttgacatcat gaacgagccg acggcggcca cactgacgta
     3901 tgcctatcgc gagggtatcc ttggaaaccc cgaggtccgc atggacaaac ccaagaaggt
     3961 cctcgtgtat gacctggggg gtggaacctt cgacgtaact gtggtcgaat acacgcccac
     4021 ccaattccgt gtgctggcga ccgacggtga cgtccagctt ggcggcgtgg actggaacga
     4081 ccgcctgctc gattatgtgg ccgacacatt caagcagcgg cacgggattg acccgcgcga
     4141 atcaccgcaa atgattcaaa tcctgcggaa cgactgcgat ctggcaaaaa tcgaactctc
     4201 ggaaaagaca gaaaccgtca tccactgtcg ttatgagggc cgggccgtcg atgtgcccat
     4261 tacccgcgac ttgttcgatt cgctcacggc ggatttgctc cagcggaccg ccgacacaac
     4321 gctcttggtg ctggaggaag ccaaggtccg tccggaagaa cttgaggcga tcgtcctcgt
     4381 gggaggatca accttgatgc cgcaggtccc gcagatgatt cggcgggttc tcaatcggga
     4441 gccctatcgc ggcatttcgc ctcataccgc cgtggcggaa ggcgccgcca ttcacgcggc
     4501 tatcctcgaa gcaaaattcc gaggcgagca aagcgaactg gctgagcgaa ttcgccgtcg
     4561 cttgcagctc gtccagcaag agaacgttaa ctcccacggc ctcggcatcg tggccctcga
     4621 tcccaaaacg ggcaaaacgg ttaatcacat tatgattccg cgaaatagtc ggattccaat
     4681 cgaggtccgt cgtatcttca agacgaccaa agacaaccag gaacggatta ctgtccaggt
     4741 tctggaaggc gatgcgcccg atcctttcgc ctgttccttg ttgggtaagt gtcgcatcac
     4801 cgatttgccg c
//
LOCUS       DAOPTR010000142         4793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_14357_length_4793_cov_3.353103, whole genome shotgun sequence.
ACCESSION   DAOPTR010000142 DAOPTR010000000
VERSION     DAOPTR010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14357_length_4793_cov_3.353103"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..1877)
                     /locus_tag="PKI05_04010"
     CDS             complement(69..1877)
                     /locus_tag="PKI05_04010"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOA51394.1"
                     /translation="MSKTIPSEPYLRERETAMYATRFGLVTALLWGVIGSVVFCATES
                     ASAEQPPDWIFHHGRIVTVDKDFRVVEAMAIRGDRIVAVGGNDEVMRLAGDQTRLVDL
                     RSKMVLPGLIDTHSHPVGAAVYEFDHEIPTMETIADVLAYIRQRAAVVPEGKWIVIQQ
                     VFVTRLKDRRFPTRQELDEAAPKHPVMFRTGPDAALNSLALQLCGIDRNFEITDGKPG
                     FVEKDPVTGEPTGILRSCTRLVKASTSARSPTPEEHREALRKLLLAYNEVGLTSITDR
                     GTTDRSIELFKQLLDRGELTCRMFLTYYVDAQEPLETIESKIKKVIAHPLHQYNPWIW
                     LRGFKFYLDGGMLTGSAYMRQPWGVSKIYGITDPEYRGVLFLEPEKLYQIVKLALANG
                     LQPTAHSVGDGAVLALAEAYARVNQDFPVKPLRPCICHANFMSPEAIDLMAKYGIVAD
                     LQPAWLYHDGATLRAHFGEERLRYFQPYRTLAERGVIVGGGSDHMQKIGRRRSINIYD
                     PWLGIWTVLTRLPRWSDSPLHPEERITRQQAIQLYTINGAYLTFEEDKKGSLEPGKYA
                     DFIIIDRDVLSCPVDDIADTQVLETWVGGRKVYPQS"
     gene            2249..2983
                     /gene="rpsB"
                     /locus_tag="PKI05_04015"
     CDS             2249..2983
                     /gene="rpsB"
                     /locus_tag="PKI05_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011122857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOA51395.1"
                     /translation="MATILVKDLIEAGVHYGHRTSRWNPKMRPYIFGRKNDIHIIDIR
                     ETLRGLLRARKYLHQLGAKGSLILFVGTKRQAADIIAEQAQRCGMPYVNDRWLGGTLT
                     NFATIRSRFQRLEELESIIHSDAINTFSKKMQSALNREYRKIYRNLHGLRTMERLPEC
                     LIIVDPNKEKNAVREARKLGITTVALIDTDCDPDDVDLPIPGNDDSMRSIELIVRALA
                     DAILEGKAAAYSKEEAAPVPSQVGVS"
     gene            3093..3920
                     /gene="tsf"
                     /locus_tag="PKI05_04020"
     CDS             3093..3920
                     /gene="tsf"
                     /locus_tag="PKI05_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOA51396.1"
                     /translation="MPEITAAAVKALRDRTGLPMMDCKRALEEAQGNPEVAIEILRKS
                     GQKVMEKRAGRETAFGRIGMYCEPRAKTALVELLCESAPVAKAEDFVALATELAKQLA
                     LGPGADSAEALLSQPSSQPGKTLRDVFEDTVNRIREAFRIGRILRIDGACGAYLHHDG
                     SKAGVVQVEGSQLDVARDIAMHVVAMRPTALRREDLDPALVAKEREILTEATRKEGKP
                     ENIIPKIVEGRLKDFYSQHCLVDQPFVKDPKTTVGKVAQAAGLTLLRYWYWEVGKES"
     gene            3924..4703
                     /gene="pyrH"
                     /locus_tag="PKI05_04025"
     CDS             3924..4703
                     /gene="pyrH"
                     /locus_tag="PKI05_04025"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOA51397.1"
                     /translation="MSAEEQQQRASPYRRVVLKISGEGFTRNNERGINMASVVELAQE
                     TYRAAKSGVELAIVLGGGNILRGAQFSAGTTSIQPATAHYMGMLATIINGLAFQDALE
                     ALGLETRLVSALFMQDVAEPNIHRRIERHLSKGRVVILTGGTGRPFVTTDTAAAQAAL
                     ELKADIVMKATKVDGVFSDDPAKNPHAILYSRITYREFLRQNLRVMDPEAVTKCSEHQ
                     LPIVVFNFRRPGNIERAVRGERVGTLIYAGDATTGGEDVSP"
BASE COUNT         1058 a         1352 c         1412 g          971 t
ORIGIN      
        1 cttgccctca ccccaaccct ctcccagagg gagagggaac tttgggatag gctcttagat
       61 caaacggcct acgattgcgg atacaccttt cggccaccga cccaggtttc gagcacttgg
      121 gtatcggcga tgtcgtccac cggacagctc aggacatccc gatcgatgat gataaaatcg
      181 gcgtacttgc cgggttcaag cgagcctttt ttgtcttcct caaatgtcaa gtaggcacca
      241 ttgatcgtgt aaagctggat ggcctgctgc cgcgtgatgc gttcctcggg atggagcggc
      301 gaatcgctcc agcggggcaa ccgggtaagg acggtccaga ttcccagcca agggtcgtaa
      361 atattgatgg accggcgtcg ccctatcttt tgcatgtgat cggacccacc gcctacgatc
      421 acgccgcgct cggccagggt tcggtacggc tggaaatagc ggagccgctc ttcgccaaaa
      481 tgggcgcgga gtgtggcgcc gtcgtggtaa agccaggcgg gctgaaggtc ggccacaata
      541 ccatacttgg ccatgaggtc aatggcttcc ggactcataa aattggcgtg acaaatgcat
      601 gggcgaagcg gtttaacggg gaaatcctgg ttgacccgcg cgtaagcctc ggccaaagcc
      661 aagaccgccc cgtcacccac cgaatgggcc gtcggttgca gaccattggc caaggccagt
      721 ttgacgattt gatagagctt ttccggttcg aggaagagca ccccgcgata ctccggatcg
      781 gtaatgccgt aaatcttgct cacgccccaa ggctgccgca tgtaggcact gcccgtgagc
      841 atgccgccat ccagatagaa cttgaatccg cgaagccaaa tccagggatt gtactgatgc
      901 agggggtggg caatgacttt cttgattttc gattcgattg tttcaagcgg ttcttgggca
      961 tccacgtaat aggttaaaaa catacggcaa gtcagctcac cgcgatccaa caactgcttg
     1021 aaaagttcga tggaacggtc agtcgttccg cggtctgtga tactcgtcaa gcccacctcg
     1081 ttatatgcca gcaacagctt ccgcaacgcc tcccgatgtt cctccggagt cggtgatcgg
     1141 gcggatgtgc tggcctttac cagtcgcgtg caactccgca agatgcccgt cggttccccg
     1201 gtgacaggat ccttctcaac gaagcccggc ttaccatctg tgatctcaaa attgcggtcg
     1261 atgccacaca attgcaaggc tagcgaattg agcgcggcgt ccggtcccgt gcggaacatg
     1321 acaggatgtt tgggagccgc ctcatctaat tcttgccgcg tcggaaaacg ccggtccttt
     1381 agccgagtga caaagacctg ctggataacg atccactttc cctcaggcac aaccgcggcc
     1441 cgttgtcgaa tgtacgcaag cacgtcggcg atcgtttcca tggtggggat ttcgtgatcg
     1501 aactcgtaaa ccgctgcccc aacaggatgc gaatgcgtgt cgatcaagcc ggggagcacc
     1561 atcttggacc gcaaatcgac cagacgggtc tgatctccgg ccagccgcat cacctcatcg
     1621 ttgccaccga cggccacaat acgatcgccg cgaatggcca tggcctccac cacacgaaag
     1681 tccttatcga cagtcacaat tcggccatgg tgaaagatcc aatccggtgg ttgctccgcc
     1741 gacgcactct ccgttgcaca gaagacgacg ctcccgatca ccccccagag gagtgccgtc
     1801 acaagcccaa agcgagtggc atacatggct gtctcccttt cccgaaggta cggttcgctc
     1861 ggtatggtct tggacacgat ttattcgatc cgcgaagcag tctcgctgcg atagaattct
     1921 agccgaatca gggccagctg caacaagcag agagaatcga gggaccggct gcgtcacctt
     1981 ttcaagggtt gatgacatcc actatgcggc gtatctcttt ccctatggga tagggaactt
     2041 tgagcgaagg cacgaccggc gggtcgattc ctcgtagggt tgtgtggcgg ttgaccggtt
     2101 ttccggaagc gggtatgcta caagtctctg ccttgagcgg cccaaggtgg gagtgcgcag
     2161 tttggtccag gccgggtaag actttcgact aacgccggac gccccagatg tggggacatc
     2221 gccggtgtgc ttgtaacagg agactggtgt ggcaacgatc cttgtaaaag acctgattga
     2281 agcgggagtt cattacggac atcggacaag tcgttggaac ccgaaaatgc ggccatacat
     2341 ctttggccga aaaaatgaca tccacatcat cgacattcgg gagactcttc ggggattatt
     2401 gcgggcgaga aaatacctgc atcaactggg ggccaagggc tccctgattc tttttgtggg
     2461 gaccaagcgg caagccgccg acatcattgc cgaacaggcc cagcgctgcg gcatgccgta
     2521 cgtgaatgat cgctggttgg gcggcacatt gacgaatttc gccacaattc gtagccggtt
     2581 ccaacggttg gaagagctgg aatccatcat tcacagcgat gccatcaaca cattctctaa
     2641 gaaaatgcag tcggccctga atcgggaata tcgcaagatt taccgcaact tgcacggatt
     2701 gcgaacgatg gagcgcctcc ccgaatgctt gatcatcgtt gacccgaaca aggaaaagaa
     2761 cgcggtccgt gaagcgcgga aactggggat cacaactgtc gccctgattg acacggattg
     2821 cgatcccgat gatgtcgatc ttcccatccc gggcaacgac gacagcatgc gttcgatcga
     2881 actcattgtg cgggcgctgg cagatgccat cttggagggc aaagccgcgg cgtactctaa
     2941 ggaggaggcc gccccggtgc cgtcacaggt cggcgtgagc tgataggctc tttaaggcaa
     3001 cgtcgccgca gatgggatgg aacggcgtca atctgccgcg cagtgtttta atgaaattgg
     3061 atcatttttc accataggtc ggaggaaatt ccatgccgga aatcaccgca gcggccgtga
     3121 aggcccttcg tgatcgtaca ggtttgccga tgatggattg caagcgggcc ttggaagagg
     3181 cccaggggaa tcccgaggtt gccatcgaga tcctgcggaa atcgggccaa aaggtcatgg
     3241 aaaagcgggc gggccgggaa accgctttcg gccggatcgg catgtactgc gaaccaaggg
     3301 cgaagacggc tctcgtggag ttactctgcg aaagtgcacc agtggccaag gccgaggatt
     3361 ttgtggccct ggccactgaa ttggccaagc aacttgcttt gggaccgggg gccgatagcg
     3421 ccgaggccct tttgagccaa ccatcgtcgc agccgggaaa gacattgcgc gatgtcttcg
     3481 aggacacggt caaccgaatc cgcgaggcat tccggattgg ccgtatcctt cgcatcgatg
     3541 gggcctgtgg agcctacctc catcatgatg gaagcaaggc cggtgttgtc caggtggaag
     3601 gctcgcaact cgatgtcgca cgggacatcg ccatgcatgt ggtggccatg cgtcccacgg
     3661 ctttgcgacg ggaagacctc gatcctgccc tggtggcgaa agagcgcgag attcttaccg
     3721 aggccacccg caaagaaggc aaacctgaga atatcatccc caagattgtg gaaggccgat
     3781 tgaaggactt ctactcccag cattgcctcg ttgatcagcc gttcgtcaaa gaccccaaga
     3841 ccaccgtggg gaaagttgct caagccgccg gtctgacgct ccttcggtac tggtattggg
     3901 aagtcggaaa ggagtcgtga tccgtgtctg cggaggaaca acaacagcgg gcatcaccct
     3961 accgccgcgt cgtgttgaag atctccgggg aaggctttac ccgaaataac gagcgtggga
     4021 tcaacatggc gtcggtggtg gaactggccc aggagaccta tcgggctgcc aagagcggtg
     4081 tagagttggc gatcgtcctg ggcggtggca atatcctccg cggtgcgcag ttttcggccg
     4141 gaaccaccag cattcaaccg gcgaccgccc actatatggg gatgctggct accatcatca
     4201 atgggctggc ttttcaggat gccttggagg cgctggggct tgagacgcgg cttgtctcgg
     4261 ccctatttat gcaggacgtc gccgaaccga acatccaccg ccgcatcgaa cgccatttga
     4321 gtaagggtcg cgtggtgatc ctcacggggg gaaccggtcg cccatttgtc accaccgaca
     4381 cggcagccgc tcaggccgcc ttggaactga aggccgatat cgtcatgaaa gcaaccaaag
     4441 tggacggcgt tttcagtgac gatccggcca agaatcccca cgccattctt tatagtcgga
     4501 tcacgtaccg ggaattcctc cggcagaacc tccgcgtcat ggacccggag gccgtcacca
     4561 agtgctccga acaccagttg cccatcgtgg ttttcaattt tcgacgaccc gggaacattg
     4621 agcgggcagt ccgtggagaa cgggtgggca ccctgattta cgcaggcgat gccaccaccg
     4681 gaggggaaga cgtcagtccg taatccgtcg gtgtgatgcc cctacgacgg cgcatggccc
     4741 tccgtgacga agtgatacat ccgtaggggc aggcccccgt gcccgcccat tgg
//
LOCUS       DAOPTR010000143         4789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_14376_length_4789_cov_3.338614, whole genome shotgun sequence.
ACCESSION   DAOPTR010000143 DAOPTR010000000
VERSION     DAOPTR010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14376_length_4789_cov_3.338614"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PKI05_04030"
     CDS             <1..542
                     /locus_tag="PKI05_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HOA51398.1"
                     /translation="KDAQAAGRIVLDEDVARRKDRLAQILATPVTDFELSVRSRNCLQ
                     KMGIMTLGDLCRCTEQDLLASKNFGETSLAEIKEMLASKGLRLGQLLPERTAAEPLGE
                     PEEPLAPEEEALLAKPVSEINLSVRARKCMARLGINTVGELIRKSGDELLECKNFGVT
                     SLNEVREKLRALGLKLRGD"
     gene            655..1791
                     /gene="tgt"
                     /locus_tag="PKI05_04035"
     CDS             655..1791
                     /gene="tgt"
                     /locus_tag="PKI05_04035"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOA51399.1"
                     /translation="MHSAQESFQVICEDPHCAARCGILRTAHGEVQTPAFMPVGTRGS
                     IKGVLPELVRRTGTQIILSNTYHLLLRPGPEIVAELGGLHRFMGWDGPILTDSGGFQV
                     FSLAQLAKIDETGATFRSHIDGQRVVLTPEGSISVQEMLGSDVAMVFDHVVGLPNERT
                     VIKEAMERSLRWAERCKVAHSRKEQLLFAIVQGGLEPDLRIRCAEALINLGFDGYAVG
                     GLSVGEPPQEMYRILDVTVPVLPRTRPRYLMGVGRPVDLIEAIYRGIDMFDCVIPTRN
                     GRNAMAFTDDGPMRMRNARYERDPRPIDPTCPCPACSFSRGYIRHLFMVKEMLGPILL
                     TLHNLTYYQRLIQRARSAICEGRFCEFRQEMLSKLAAGNDANDT"
     gene            complement(1847..2935)
                     /locus_tag="PKI05_04040"
     CDS             complement(1847..2935)
                     /locus_tag="PKI05_04040"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOA51400.1"
                     /translation="MSENSPLVRQWILLRMLASRRFGVTVREMAQTLEVSEKTIRRDL
                     DMFIQAGFPLDETVGEHGKKRWRLTGDLIEKGLPITYDEAIALHLARCLMEPLAGTMF
                     WTAAQSAFNKIRSVFNETAWAYVEKMAHSFYRTRTGMCDYAKKAELIDQIMLAIEDRK
                     AIELRYHSLKAPEATTYTVHPYGLTYHRGALYLVGLLVECNEIRHWKVNRVEAVKITD
                     QPFDFPEGFSLEKHLAGSFGIFQGDGDYHVRVLFSPVVARYVSEATWHASQRLYPQRD
                     GSVIAEFDLDSLEEIQRWILSFGKYAKVLEPEELQQRMQEEIREMLATYEDLRERPSR
                     KRQLSPHAKLAFAAPDFGPAFAPIERTP"
     gene            complement(3300..>4789)
                     /locus_tag="PKI05_04045"
     CDS             complement(3300..>4789)
                     /locus_tag="PKI05_04045"
                     /inference="COORDINATES: protein motif:HMM:NF023591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51401.1"
                     /translation="FNALLKTLEEPPEHVKFIFCTTEPNKVPVTILSRCQRFDFAGIE
                     TQTIVARLQQIVEAEGATVEPGVLELLARRAAGSMRDAQSLLEQLLSFSGGKNIAVSD
                     VLELLGMARAEVFKAILEAATKEDASQALAVFHQALKDGADAGLLIEQLLTYLRDCMV
                     ATLGCSGDMLLSVGPGELSVASELGQRLGLPRLLASMQLLDHTLARMRFATHDRILAE
                     LAIVRLSRLGELEYVGKLIEAIRSGKSLPTVATPDQARSVEGIGISQPLHQAPKTLAS
                     ELAGVLGRPSPGSPQPPAGDQQGAAQARQAVEQAKAELERQKEAVKASVPREGTLPAY
                     PSGEALTSENVLNVWVKAAESVGGLAADQAKQFSQVAVAGGNRVVVTFRKDKAFCRDY
                     CSRPEIKTRFERALQTITGNKIELELVLANEGAPEKSSPGTGERLLFDILQEPFVRKT
                     AELFGARPIAVLPPEERKTTVTQAPIEEDRSLDEEHHVEEDEDDV"
BASE COUNT         1074 a         1325 c         1283 g         1107 t
ORIGIN      
        1 tgaaggatgc ccaggccgcg ggacgcatcg tgttagacga ggatgtggct cgccgaaagg
       61 accggctcgc ccaaattttg gcgacacccg tgacagattt cgagttgtct gttcgaagtc
      121 gaaattgcct gcaaaagatg ggcattatga cattgggcga cctgtgtcgc tgcacagaac
      181 aggatttgct cgccagtaag aatttcggtg agaccagtct tgccgagatc aaggagatgt
      241 tggcctcaaa gggactgcgt ctcggtcaac tccttcccga acgcacggct gcagaacccc
      301 tcggagaacc cgaggagccg ctcgctcccg aagaagaggc tttgctggcc aagcccgtct
      361 cggagataaa tctttcggtg cgggcgagaa aatgcatggc gcgccttggg atcaataccg
      421 ttggtgagct gatccgcaaa agtggcgacg aacttttgga atgcaaaaac tttggcgtca
      481 caagcctcaa tgaagttcgc gaaaagttgc gggctttagg gttgaaattg cgtggtgatt
      541 gagacccgaa cggtggtgtg gggtaggcga caattccaca aactccggcg aaggccagcg
      601 gagcctttat tgctggtgat ttgtctggac ctgggctgtt gatgtactcc tttgatgcac
      661 tcggcgcaag agtcgtttca ggttatctgc gaagacccac actgtgctgc ccggtgcggc
      721 atcctaagaa cggcccatgg tgaagtgcag actcccgctt tcatgcccgt aggaacgcgg
      781 ggttcgatca agggtgtgtt gccggagttg gtccgtcgga ccgggacgca gatcatcctt
      841 tcgaacacct accatttact gttgaggccc ggtcctgaga tcgttgccga gttaggcggt
      901 ttgcatcgtt ttatggggtg ggacgggcct attcttacag acagcggcgg atttcaggtc
      961 ttcagtctgg cacagcttgc aaagatcgac gaaactgggg ccacctttcg ctcccatatt
     1021 gatggacagc gtgtggtctt aacgccggaa ggctccatct cggttcagga gatgctaggc
     1081 tctgacgtgg ccatggtttt tgaccacgtg gtggggctgc ctaacgagcg gaccgttatc
     1141 aaagaagcca tggaacggag tcttcgctgg gctgagcgct gcaaggttgc ccactccaga
     1201 aaggaacagt tactttttgc cattgttcaa ggtggtctcg agccggattt gaggatccga
     1261 tgcgctgaag ccctaattaa cctggggttt gacggttatg ccgttggcgg tttgagtgta
     1321 ggggagccac cacaggagat gtatcgcatt cttgacgtga cggtccccgt gctgccaaga
     1381 acacgccccc ggtatcttat gggcgttggg cgtcccgttg atttgattga ggccatttat
     1441 cgcgggatcg acatgttcga ttgcgtgatc ccaacgcgga atggacgcaa cgctatggca
     1501 ttcaccgatg atgggccaat gcgtatgagg aatgctcgct atgagcgaga ccctcgcccc
     1561 atcgacccca cgtgtccttg tcctgcctgt tctttcagcc gcggctacat tcgacatttg
     1621 tttatggtca aagaaatgct cggccctatc cttcttactc tgcataacct gacgtactac
     1681 cagcgtctga tccagcgggc ccgaagtgcc atctgcgagg gccgtttttg cgaattcagg
     1741 caggaaatgc tatccaaact cgctgcggga aacgacgcca atgatacata aatggctccg
     1801 cacggcggga aaaaattggt taccagacag gaaagaaagc tatccactac ggcgttcgtt
     1861 cgatcggggc gaacgcggga ccgaagtcag gcgcggcaaa agcaagcttc gcgtgaggtg
     1921 acaactgtcg cttccttgag ggccgctccc gcaagtcttc gtaggtggct aacatctccc
     1981 taatctcttc ctgcatccgc tgttgtaact cctcaggttc aaggaccttg gcatatttcc
     2041 caaaactaag aatccaccgt tggatttcct ccaaactgtc cagatcaaac tcggcaatga
     2101 cggaaccatc acgctgggga tatagccgct ggctggcatg ccatgtcgct tcgcttacat
     2161 agcgcgcaac gacgggagaa aacaaaaccc gcacatgata atcgccatcg ccctggaaga
     2221 taccgaaact gccggcaaga tgtttttcaa gggaaaatcc ttctgggaag tcaaacggct
     2281 gatcggttat tttgacagcc tcaacgcgat tgaccttcca atggcgaatt tcgttgcatt
     2341 caaccaagag tcccacaaga taaagtgccc ctcggtgata ggtcaaccca tagggatgca
     2401 ccgtgtacgt ggtggcttcc ggcgccttca acgaatggta tcgcaattca atagccttcc
     2461 ggtcttcaat ggcaagcata atctggtcga tgagctcagc cttcttggcg taatcgcaca
     2521 tccccgtccg ggttctataa aaagaatggg ccatcttttc cacgtaggcc caagccgtct
     2581 cattgaaaac actgcggatt ttgttgaagg cactttgcgc agccgtccag aacatcgtcc
     2641 ccgcgagagg ttccatcaga caccgtgcaa gatgcaaggc aatcgcctca tcgtaagtga
     2701 taggtaatcc tttttctatg agatcaccgg ttagcctcca acgtttcttg ccatgttcac
     2761 caacagtctc atctaaggga aaacccgctt gtatgaacat gtcgagatca cgacgaatcg
     2821 ttttctcgct cacttccagg gtctgggcca tttcgcgaac cgtgacaccg aaccgccgtg
     2881 acgccagcat gcggagaaga atccattgac gcaccaaggg tgagttttcg gacatggcac
     2941 atccttcgtc aattgggtta acccggtaga cgcgatgagt tggcatctgc tcaaccgacc
     3001 accaggatcg atccgaattt ccctttacgt tcgatcatat actcactgaa gcttgaagtg
     3061 aattccgccg cttgatgaag aagcgattca ggatatgcac acgagaagct actgacagcg
     3121 tgtagtcatc ctatggaaaa gtgggatcgc caaatgccag aaatgggcct ctttgccccg
     3181 gttgaccgtc gaaagccccc gacgacgcaa tcctactctc gtcaatccgc tcagcaaacc
     3241 cgcattcaga acattcaggc tactcttgct ctgcgatatg aaactgccag agtcaggaac
     3301 taaacgtcat cttcatcctc ttcgacatgg tgttcttcgt cgagcgagcg gtcttcttcg
     3361 attggggcct gtgtcactgt ggtttttcgt tcctcgggcg gcaggacggc gatgggtcgc
     3421 gcaccaaaca gctcggcagt tttcctcaca aatggctcct gaaggatatc aaaaagtaac
     3481 cgttcgcctg tccccgggct tgatttctcc ggcgcacctt cgtttgccag aactagttct
     3541 agctcaattt tattgccggt gatcgtctgg agagcccttt caaatcgggt cttgatttct
     3601 ggacgcgaac aataatccct acagaacgcc ttatctttgc ggaacgtcac gacgacccgg
     3661 ttaccgccgg cgactgccac ctgggagaat tgcttcgcct gatcggctgc cagtcccccc
     3721 acagactccg ctgctttaac ccagacattg agaacatttt cactcgtgag tgcctcgccc
     3781 gatgggtaag ccggtaatgt cccttcgcgt ggcactgaag ccttgactgc ctctttttgc
     3841 ctttccaatt cagccttggc ttgctcaacg gcctgcctcg cttgtgcggc accctgttga
     3901 tcgccagccg gaggttgtgg cgaaccagga cttggtctcc ccaagactcc tgccaactca
     3961 ctggcgagcg tttttggtgc ttgatgcaac ggttgtgaga taccaatccc ctcgacagaa
     4021 cgggcctgat ccggcgttgc gacagtcggc aagctcttgc cggaccgtat ggcttcaatc
     4081 agtttgccta cgtactccaa ttctcccagc cgggacaatc ggacgatggc caactcggcg
     4141 agaatccggt cgtgggtagc aaaccgcatc cgtgccaagg tgtgatcaag aagctgcatt
     4201 gacgccagca gtcgtggtag ccccaatcgc tgtcccaact ccgaggctac tgaaagttcc
     4261 ccaggcccca cagaaagaag catgtctcct gagcaaccca atgtcgcaac catgcagtca
     4321 cgcaggtaag tgaggagctg ctcaattaaa agccccgcat ccgcgccgtc cttaagggct
     4381 tgatgaaata ccgccagagc ctggctggca tcttcctttg tggctgcctc caaaatggcc
     4441 ttgaagactt cagcccgggc catccctagg agctcaagca cgtccgatac ggcaatattc
     4501 ttcccaccgc taaaagaaag caactgctcg aggagcgact gggcatcgcg catcgaaccg
     4561 gcggcacgtc gcgcgaggag ctccaagacg cccggctcta cggtggcccc ctcggcttcg
     4621 acaatctgct gcaatcgcgc tacaatggtc tgtgtttcga tccctgcgaa atcgaatcgc
     4681 tggcaccgcg aaaggattgt gacgggaacc ttgttcggct cagtcgtgca aaagataaat
     4741 ttcacatgct cgggaggctc ttccagcgtc ttgagaaggg cgttaaaag
//
LOCUS       DAOPTR010000144         4783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_14402_length_4783_cov_3.767978, whole genome shotgun sequence.
ACCESSION   DAOPTR010000144 DAOPTR010000000
VERSION     DAOPTR010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14402_length_4783_cov_3.767978"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..3972
                     /locus_tag="PKI05_04050"
     CDS             268..3972
                     /locus_tag="PKI05_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008653751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOA51402.1"
                     /translation="MELLERIWEIVSDTFVVVGQAVDRGITSLFGSANARYLRRLQPK
                     VQAINDLEPRYQRMTDAELREQTAKFRKRLEAGETLDDLLVEAFAVCREASRRVLGMR
                     HFDVQLMGGMVLHAGAIAEMVTGEGKTLVATLPAYLNALEGKGVHVVTVNDYLARRDM
                     EWMGPLYMFLGLTVGAIQHDMGARERQQAYSCDITYGTNNEFGFDYRRDNMRLAAKGD
                     NRYPPEYQQVQGRLHYAIIDEVDNILIDEARTPLIISGPAHDDISRYKRADQIARQLI
                     RDVHFVVNEKDHTCHLTEEGIRKAEELAGVESFYTAGNLEWPHLIDNALKAHHLYKRD
                     VHYVIENNQVIIVDEFTGRLMYGRQWSDGLHQAVEAKEGVPIKEETQTLATITIQNFF
                     RLYDKICGMTGTAMTEANEFWKIYKLEVVAIPTNKPLRRINHPDVIYRTAREKYKAIV
                     DEIERMHKWDTVWYSRYEYEVGRIVEEDDERVVFVTREGDRKVIPRSQIHNIEKKGRP
                     ILVGTVSVEKSEMLSEMLDRRGIPHQVLNAKHHAREAEIIAQAGRKGAVTIATNMAGR
                     GTDIILGGNPEAKAWSILQHKYRSRLDVPRDEWDALVKEIEEKEQMKAEGREVAALGG
                     LHVIGTERHEARRIDNQLRGRAGRQGDPGSSRFYLSLEDDLMRIFAGDWVKNILTRLG
                     MEEGQAIESPMVTRRIEAAQKKVEERNFEIRKSLLEFDQVMDEQRKRVYGYRQSILEG
                     ADCRSLIRDMIRQEIDKNLDRFLDKSYGAETFAQWISKQLSVEFRARDFRGMDFPTAE
                     AYARDHAERMAESQIFDLIEENLPEEAERTDWNWEALTKAVNTRWRLDLKDRELRKIP
                     REDLSEYLIEKAQQSIRTIDLSEGAKYLDPDFGLKSAINWVRSKFGIELTVEELRDLE
                     PSAIKDLVFEKALKAYQQKEIEFPVRAAFSHYTGRDATGRRVYDREGIIRWAKGRLGV
                     ELTEEDLRTKTGQDIWNLLVEASEKDYLKAEALRTEGEALLASLYEGLGDDPRVKCDR
                     NHPKLGEFCRWVAEHLGLEMTPESVARMERRDLQWKFRDAFNDRYSPEMRQMERFLLL
                     HTLDSAWKEHLLVMDHLRSSVSLRGYAQVDPKVEYKREGMRQFELMWESVGRRVTDLV
                     FRMEQMDERFVGSMWKETAAERPEASPAVTASVATLGSQQQEAIENSQAGSKAKPIRK
                     SGPEIGRNDPCPCGSGKKYKHCCMRNRRPA"
     gene            4084..>4783
                     /locus_tag="PKI05_04055"
     CDS             4084..>4783
                     /locus_tag="PKI05_04055"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOA51403.1"
                     /translation="MFWLMDLVYFVLLLVTAPIWVTRLVLHRRKYQHGWAEKLLGLVP
                     CRNSNAPCIWFHAVSVGEVNLLATLLAEMRRRLPNYDICLSTTTQTGMEVAKKKYPEL
                     LLFYCPMDFSWAVRRAMRRIRPNLLVLAELELWPNLIRAADEYGARVAVVNGRLSDRS
                     FRGYQRIRWVFAKTLRRIALVAAQDPTTARRFIELGVPPDRVAVTGSLKFDGTEFDRN
                     NPRTQRLRDLARIRT"
BASE COUNT         1156 a         1234 c         1389 g         1004 t
ORIGIN      
        1 tataagatta aattaagcaa attggtgcca tttgttcgtc gcccatcccc aggcggcccg
       61 cgtggattct cttatgagag gcttagcccg tgtcttttgc tacagcctca gtggttggtt
      121 gtgtcttcat gataccgctc gcatctcccc actgatgatg gtcttaagac acagcccatg
      181 cacgcggagc cgtcaccgga tcggtgcttg aggtaactaa gaaaacaaac aaaacttgac
      241 tggaatatca agggcctttg gagagccatg gaattattgg aaagaatttg ggaaatcgtc
      301 tcggacacct tcgtcgtggt gggtcaggcc gtggaccgcg gcattacgtc gctgtttggc
      361 tcggccaacg cccgctatct gcgacgcctt cagccgaagg tccaggccat caatgatcta
      421 gagccccgct atcagcgtat gaccgacgct gaattacggg aacaaacggc gaagtttcgc
      481 aaacgactgg aagcgggcga gacgctggat gatctcctcg ttgaagcctt tgccgtttgc
      541 cgggaggcct cccgccgtgt cctggggatg cggcatttcg atgtgcagtt gatgggggga
      601 atggttctcc acgccggggc aattgccgaa atggttaccg gcgagggtaa aaccctcgtg
      661 gccacgctgc ccgcctatct gaatgccctc gagggcaaag gcgtgcacgt cgtcacggtg
      721 aacgactatt tggcccggcg agacatggag tggatgggcc cactgtacat gttcttgggc
      781 ctaacggtgg gggccattca acacgacatg ggtgccaggg aacggcaaca ggcctactct
      841 tgtgacatca cgtacgggac gaacaacgag ttcggttttg attaccgacg ggataacatg
      901 cggttggcgg caaaaggcga caaccggtat ccccccgagt atcagcaggt ccaaggtcgt
      961 cttcactacg ccattatcga cgaggtggat aacatcctta tcgacgaggc gcgaaccccg
     1021 cttatcattt cgggaccggc ccacgacgac atctcccggt acaagcgggc agatcagatc
     1081 gctcgacaac tgatccgcga cgtccatttt gtggtcaacg agaaggatca cacgtgccac
     1141 ctcaccgaag aagggatcag aaaagcggaa gaactggccg gggtcgagag tttctacacg
     1201 gctggtaatc ttgaatggcc ccacctaatc gacaatgccc tcaaagcgca ccatctttat
     1261 aagcgcgatg tgcattatgt catcgagaac aaccaggtga tcattgtcga tgagttcacc
     1321 gggcgtctca tgtacggtcg tcaatggagt gatggtcttc accaagctgt ggaggcgaaa
     1381 gagggcgtcc ccatcaagga agaaacgcaa acgttggcga caatcaccat tcagaacttc
     1441 ttccggttgt acgacaagat ttgtggtatg accggaaccg ccatgacgga ggccaacgaa
     1501 ttctggaaaa tctacaaact cgaggtcgtg gcaatcccca ccaacaagcc ccttcggcgg
     1561 atcaaccatc ccgatgttat ctaccggacg gcgcgagaga agtacaaggc cattgtggac
     1621 gaaattgagc ggatgcacaa atgggacacc gtttggtatt cccgttatga atatgaggtt
     1681 ggtcgaattg tcgaagaaga tgatgaaagg gttgtgttcg taacgcgaga aggtgatcgc
     1741 aaggttatcc cgcgatcgca aatccacaac atcgaaaaga agggccgccc gattctcgtg
     1801 ggaaccgtgt cggtcgaaaa gagcgagatg ctttccgaga tgttggaccg tcgcggcatc
     1861 ccgcaccagg tcctcaatgc caagcatcat gcgcgggaag ccgagattat cgcccaagct
     1921 gggcgtaaag gcgcggtaac cattgccaca aacatggccg ggcgtgggac cgacatcatt
     1981 cttggtggaa accccgaggc caaggcctgg tccatccttc agcacaagta tcgcagccgg
     2041 ctagatgtcc cgcgtgatga atgggacgcc ttagtcaagg aaatcgaaga aaaggaacag
     2101 atgaaggcgg aaggccggga ggtcgccgcc ctagggggct tgcacgtcat cggtacggaa
     2161 cggcacgagg cacgccgcat cgacaaccag ctccgcggcc gtgcaggacg ccaaggggat
     2221 ccaggaagta gccggtttta tctctcgcta gaagacgatt tgatgcggat ttttgcggga
     2281 gattgggtca agaatatcct cacgcggcta gggatggagg aaggccaggc gattgaaagc
     2341 cccatggtga ctcgtcgcat tgaagccgcc caaaagaagg tcgaggagcg aaactttgaa
     2401 atccgcaaga gcctcctcga attcgatcag gtcatggatg agcagcgaaa gcgtgtttat
     2461 ggctatcggc aaagcatcct ggaaggggcg gattgtcgta gtcttattcg cgatatgatc
     2521 cgccaagaga tcgacaaaaa cttagaccgt tttctggaca aaagctacgg ggcagagacg
     2581 tttgcccagt ggatcagcaa gcaactgtcc gttgaatttc gggcgcgaga ttttcgcggc
     2641 atggattttc ccacagccga ggcctacgcc agggatcatg ccgaacggat ggcggaaagt
     2701 caaatctttg atttgatcga agaaaacttg ccggaagagg ctgagagaac agactggaat
     2761 tgggaagcgc tgacgaaagc tgtcaacacc cgctggcggc tcgatctcaa ggaccgagag
     2821 ttgagaaaaa tcccccgaga agatttgtcg gagtacctca tcgaaaaggc ccaacaatcg
     2881 atccgcacca ttgatctaag cgagggagcg aagtacctcg atcccgattt tggcctcaag
     2941 tcagccatca attgggttcg cagcaaattt ggcattgaac tcaccgtcga agagctgcgt
     3001 gatctcgaac cttctgccat caaagacctt gtttttgaaa aggcattgaa ggcctatcaa
     3061 caaaaagaga tcgaatttcc ggtgcgggct gcctttagcc attacacagg ccgcgacgcc
     3121 acagggcgac gggtttatga ccgagagggt atcattcgct gggcgaaggg acgtttgggt
     3181 gttgaattga cggaggaaga ccttcgcact aagacgggcc aggatatttg gaacctcttg
     3241 gtggaggcca gtgaaaagga ttatctcaag gcagaagccc ttcgcactga gggagaggcc
     3301 cttttggcat cgctctatga ggggctcggc gacgaccctc gcgtcaaatg tgatcgcaac
     3361 catcctaaat tgggcgagtt ctgccggtgg gtagcagagc acttgggcct cgaaatgaca
     3421 cctgagagtg tcgcccgtat ggaacgccgt gatctccaat ggaagttccg tgacgcgttt
     3481 aatgatcgct attcaccaga aatgcgtcag atggagcgat tcctccttct tcatactctc
     3541 gatagcgcct ggaaggagca cctccttgtg atggaccatc tccgcagtag tgttagcctg
     3601 cggggttacg cacaggtcga tcccaaggtc gaatacaagc gagaaggaat gcggcagttt
     3661 gaactgatgt gggagtctgt ggggcgacga gttaccgatt tggtattcag aatggagcaa
     3721 atggacgaac gattcgtggg ctccatgtgg aaggaaacag ccgccgagcg ccccgaggcc
     3781 tctccggccg tcactgctag cgtggcaacc ctgggaagtc agcagcaaga ggcgatcgaa
     3841 aacagccagg cgggttctaa ggccaagcct atccgcaaga gtggacccga aattggtcga
     3901 aatgacccat gtccttgcgg aagcggtaaa aagtataaac attgctgcat gcggaatcgc
     3961 cggccggcgt agtgtggttg cagccggggt ttctcgggtt ggagtcagcg aggaatctgc
     4021 catgtgaccc cttccagcga tcgaacccaa ttcggtttga aaccatgagg gaaaggattc
     4081 ctaatgtttt ggttgatgga tcttgtctat tttgtcttac tccttgtgac ggcacccatt
     4141 tgggtaactc gtctggtcct ccatcggcga aagtaccagc atggctgggc tgaaaagctc
     4201 ctcggtttgg ttccttgccg caattcaaat gcaccatgca tttggtttca tgccgttagt
     4261 gttggagagg tcaatctgct tgcgacgttg cttgccgaaa tgcgccggcg tcttcccaat
     4321 tatgacatct gtctaagtac caccacccaa actgggatgg aggtcgcgaa gaaaaaatat
     4381 ccggaactcc tcctctttta ttgtcccatg gattttagct gggctgtccg ccgggccatg
     4441 cgacggattc gcccgaatct ccttgtcctg gccgaactgg aactctggcc caacctgatc
     4501 cgggcagccg atgaatacgg ggcgcgcgtc gccgtcgtga atggacgact gagcgatcgg
     4561 agcttccgcg gttatcaacg gattcgctgg gtcttcgcta agactctccg ccggatcgcg
     4621 ctggttgcgg cccaagaccc aaccacggcg cgacgattta tcgaattggg agtaccgccc
     4681 gaccgcgtcg cagtcaccgg atccttgaaa ttcgatggca cggaattcga ccgtaacaac
     4741 ccacgaacac agcggctccg cgacctggcc aggattcgga ccg
//
LOCUS       DAOPTR010000145         4776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_14440_length_4776_cov_3.436772, whole genome shotgun sequence.
ACCESSION   DAOPTR010000145 DAOPTR010000000
VERSION     DAOPTR010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14440_length_4776_cov_3.436772"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PKI05_04060"
     CDS             complement(<1..593)
                     /locus_tag="PKI05_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51404.1"
                     /translation="MNLIGKIFVVLIFVMSLVFMSFAIMVYSAHINWREVVENPKATP
                     DKPLGLRLQVQNLRKDNDALKAQLDELTKKLDAEIADKRAQVAKLEQERDDLQAERDQ
                     LMAQVAQLTQQTRDAVAAMEAAHQTLAKRTDEVGNLRDLLRQAEQARHDALVRLVQRT
                     DELHQVANELRALKERAVTLAADLAKAREVLTKFDLKA"
     gene            complement(906..1916)
                     /locus_tag="PKI05_04065"
     CDS             complement(906..1916)
                     /locus_tag="PKI05_04065"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOA51405.1"
                     /translation="MNVSLTLRRILWKTLLTAIFVSIICMNIPGVAVFGGTLAENAAA
                     AEKATKKETKPDDGAKPEKHEKTEYWLGITCGPIPDFLRAHVAIPENEGILVHAVAKN
                     SPAERAGICQHDILLAAANKVLKSPEDLVAVVNEKKDQSFEIKLLRKGKTETVTVTAE
                     PRPAEAKEEAPPFPGPWAPTEAWEKWFEWFRKQLPNQPPLTFRFYRPGIVVPPGEGED
                     WPKDLTIVITKEGSQPAKIVVRRGDKTWEVTEKELDKLPKDIRPHVERMLRGSRGIPG
                     GLVEVKPFGQLTPPKELEKDLVRPFRGWLSPDTERLERIEKRLEELNRRLDELQKKDK
                     SE"
     gene            complement(1944..2837)
                     /locus_tag="PKI05_04070"
     CDS             complement(1944..2837)
                     /locus_tag="PKI05_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51406.1"
                     /translation="MTIPSREEQASAPELEGFIMDQFDNEDISQEIIDRLVDGELDET
                     VRAAVLRRLEADPLLCKRIAIAFLENQAWREAFSTYCSALAPRTKSEGSLGTQFFEKR
                     RREAQSNEIGPGEALSKDTLPQIIKPSADMPRRCPQVSTLGWLVAVAASFILAFLASW
                     YIQSFWLTGARYKPTLSAGSLAANQGQVSSENQAVPVKPDGPMAVSPHIEMVSVPLPV
                     DDAGTWATMEVPFISFPGAEVTEVLPGGMIPTEILESLGAQGHVVEQERRFVPVALPD
                     GRQSVVPIDQVRIRFVDRPKR"
     gene            complement(2847..3434)
                     /locus_tag="PKI05_04075"
     CDS             complement(2847..3434)
                     /locus_tag="PKI05_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOA51407.1"
                     /translation="MGMDDADHTRATVSASVDWAEALRQRERWLRAVIFARVREAQAV
                     DDVFQEVALAAVEQKAPIQDPAKVSQWLYRLAVLTSLMFRRRQGRRRKWIRRYAEDLR
                     PHTRDGYDPLAWLISSERREAVRQALDRLPAKDVEILLLKYGQDWRYEEIAEHLGIST
                     SAVQTRLFRARQRLRRELDQMAHADSIGNESGHHT"
     gene            complement(3868..>4776)
                     /locus_tag="PKI05_04080"
     CDS             complement(3868..>4776)
                     /locus_tag="PKI05_04080"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA51408.1"
                     /translation="TPPHFRPEHLMAAVNAGKHVFMEKPVAVDVPGVKTVLQAGQIAK
                     QKNLGIAAGTQRRHERRYQETIKRIQDGAIGEIIYAKCYWNGGEIWVVEREPHWSDME
                     WQLRNWNYFCWLSGDHIVEQHVHNLDVMNWVLGAHPVRAVSGLGGRQVRTDPKYGNIY
                     DHFAVEFEYPNGVTVFSQCRQINGCLNIVGEFVLGTKGHSDCQQTIQTGSERWRYRGP
                     NPNPYEQEHEDLIASIRAGQPINEAEAVAYSTMTGILGREAVYSGQAITWDDAMQSTT
                     RLGPERYEFGPYPVQPVPLPGQYRFS"
BASE COUNT          992 a         1417 c         1227 g         1140 t
ORIGIN      
        1 gctttaagat cgaacttggt gagaacctct ctggccttag ccaagtcagc agcaagcgtg
       61 accgcccgct ccttgagggc cctcagctcg ttcgcaactt gatgaagctc gtcggtccgt
      121 tgaaccagtc ggacaagggc atcgtgcctc gcttgctcgg cttgccgaag caaatcacgc
      181 aagttgccga cctcgtccgt ccgcttggcc agcgtttgat gggccgcctc catcgccgcg
      241 acagcatcgc gggtctgttg agtgagttga gccacttggg ccatcaattg atcacgttcg
      301 gcttgtaaat catctcgttc ctgctctagc ttcgccactt gcgcccgctt gtcggcgatt
      361 tccgcatcta gcttcttggt caactcgtcg agctgggctt ttaacgcatc gttgtcttta
      421 cggaggttct gaacctgcaa gcgtaaccct agaggcttat ccggagtcgc cttgggattc
      481 tccaccacct cgcgccagtt gatgtgggcg gagtacacca taatggcgaa ggacatgaac
      541 acgagactca tcacaaagat gagcaccacg aagattttgc caatcaaatt cataggctca
      601 accttccacc accgaaaaaa gctcctaaca ccgttttgct cggagcccac gaaatcgcac
      661 tactccgaga acgtttacgc cacgccgttt tagctatccc attctacgct tcgagtataa
      721 ctagccggtc ttacccatgt caaacaattg ccgtcgaatc ccgacaacga tttacccccc
      781 tgcccctccg ttggagcggc tcaaagctca ttagaaccat gcgcaacgaa aaccccctca
      841 tacgaggggg taaatgtggg ttttgctttc tcgacgttag cttacttttt ctcggagctt
      901 catcatcact cggacttgtc ctttttttgg agttcgtcaa gtcggcgatt gagctcctca
      961 agtcgtttct cgatcctctc tagccgttcc gtatccggtg agagccagcc gcggaaggga
     1021 cgcaccaaat ccttctccaa ctccttcggt ggtgttagtt gcccaaaagg cttgacctca
     1081 accagtccgc ccggtatccc ccggctgccc cgcagcattc gctcgacgtg cggtctgatg
     1141 tctttgggga gtttatccaa ttctttttcc gtcacttccc aggtcttatc accacggcgg
     1201 acgacgattt ttgccggttg agatccctcc ttagttatga cgatcgtcaa gtctttgggc
     1261 cagtcttctc cttcccccgg gggaaccacg atccccggcc gatagaaccg aaatgtcaac
     1321 ggtggttggt taggcaattg ctttcggaac cactcgaacc acttctccca tgcctcggta
     1381 ggcgcccacg ggccggggaa tggcggggct tcctcctttg cctcagcagg ccgaggttcg
     1441 gcagtcactg tgacggtctc tgtcttaccc tttcggagaa gcttaatctc gaatgactga
     1501 tctttctttt cattgaccac agcgacgagg tcctctggcg acttgagcac cttattggcg
     1561 gctgcaagta agatgtcatg ctggcaaatt cctgcccgct ctgctggact gttctttgcg
     1621 accgcgtgga caaggatccc ctcattttcc ggaatggcca catgggcacg gagaaagtcc
     1681 gggatgggac cacaagtaat ccctagccag tattcggttt tctcgtgttt ttcaggcttg
     1741 gccccatcgt ccggctttgt ctcctttttc gtagcctttt ccgccgcagc ggcgttctcc
     1801 gcaagcgtgc caccgaaaac ggcaacacct gggatgttca tgcagatgat cgacacgaag
     1861 atcgctgtga gtagcgtttt ccacaagatt ctccttagcg tgagtgacac gttcatggcc
     1921 gatacctcct caatctgcaa accttacctc ttgggtcgat caacaaaacg aatccgcact
     1981 tggtcaattg ggacgacact ctggcggccg tcaggtaggg caacggggac gaaccgccgt
     2041 tcctgttcca caacatggcc ctgggcaccc aatgattcga gaatctcggt tggaatcatc
     2101 ccgcctggaa gcacttcagt aacctctgcc ccggggaagg aaatgaaggg gacttccatc
     2161 gttgcccagg tacccgcatc gtcgacgggc aaaggcaccg acaccatctc gatatggggt
     2221 gaaactgcca tgggcccatc gggcttgacg ggaacagcct gattttcgct cgatacctgt
     2281 ccctggttgg ccgccaacga gccggcactc aacgtgggtt tgtatcttgc cccggtcaac
     2341 cagaaggact ggatatacca tgatgccaag aatgcgagta tgaagctggc agccacagcc
     2401 acaagccatc ccaaggtgga gacttgggga cagcggcgag gcatgtcggc ggatggctta
     2461 attatttgtg gcaaagtatc tttcgaaagc gcttcacctg gcccaatttc gttggattgg
     2521 gcctctctgc gtcgcttctc aaagaactga gttccaaggg aaccttcgga cttggttctt
     2581 ggtgccaaag cagaacaata agttgaaaac gcctctcgcc aggcctgatt ttccaaaaaa
     2641 gcaatagcta tccgcttgca caaaagcggg tctgcttcca accggcgcaa aacggcggca
     2701 cggacggtct catccaattc gccatccaca aggcggtcga tgatctcttg agaaatgtcc
     2761 tcgttgtcga attgatccat aataaatccc tccaattcgg gtgcagaagc ttgttcctcc
     2821 cgactcggaa tggtcatgat aacgtcttaa gtatgatggc cactctcgtt accaattgaa
     2881 tccgcgtgtg ccatctggtc cagctcccgc cggagacgct gccgagcccg aaacagtcgg
     2941 gtttgcactg cggacgtact gattcccaaa tgctcggcaa tctcttcgta tcgccaatcc
     3001 tgaccatatt tcagtagcag gatttccaca tctttggccg gaagtcggtc caacgcttgt
     3061 cgcaccgctt cccgacgctc cgaagaaatt agccacgcca acgggtcgta accgtccctg
     3121 gtgtgaggcc tcaagtcctc ggcgtagcga cgtatccatt tccgccgtcg cccttgccga
     3181 cgtcggaaca tcaaggaagt caaaacggcg aggcggtaaa gccactggct taccttcgcc
     3241 ggatcctgga taggtgcctt ttgttccacc gcggccaggg caacctcttg gaaaacatcg
     3301 tcaacggctt gagcctctcg gacccgggcg aaaatcaccg cgcgcaacca ccgctcgcgc
     3361 tgcctgagcg cctccgccca gtcaacgcta gccgaaacgg ttgcccgagt gtgatctgcg
     3421 tcatccattc ccatgaactt tactgctgat ccgagaagaa tgttgccgct ggtgcccctg
     3481 ctgtttcggc acctgagtac cggcaacaac gagatatgtt aaccggcagg gcgaccctct
     3541 tgaggttcgc tatgccacag ccgtggtatg ccgtacccct tgctagcgct acccagagtt
     3601 ggttcacgct tgtgttcact gaaaggggct tctgaagcgt ttcaggattg cgaaaacgct
     3661 cccccggcgt ccatcgtagt gaccaggcaa ttgacctatc ggacgttacg ccgaggtcgt
     3721 ccaggagtcg tctcgactat ctttggtgac gcgcttatga gagcacgcaa accaaacgag
     3781 caccgtcacc acaaagggtg ccggtgtcgc attaatgaag cctttcggtt agtcgccgtg
     3841 gtacggcgcg attcagagcg ccctgtctca agaaaaacga tattggccag gcaagggaac
     3901 cggctggacc ggatagggac cgaattcata ccgttctggt ccaagtcgtg ttgtggactg
     3961 catggcgtcg tcccaggtga ttgcctggcc gctgtaaacc gcttctcgtc cgagaattcc
     4021 cgtcatcgtg ctataggcca cggcctccgc ttcattgatg ggttggcctg cccggatact
     4081 cgcgatcaag tcttcgtgct cctgttcgta tggattgggg ttcggcccgc ggtaacgcca
     4141 ccgctcgctg ccggtctgaa tggtttgctg gcaatcactg tggccttttg tgccgagaac
     4201 gaattcccca acgatgttaa ggcaaccgtt gatctggcga cactgactga agacggtgac
     4261 tccattgggg tactcgaatt ccacggcaaa gtggtcgtag atattgccat atttcgggtc
     4321 ggtccgcacc tgtcgtccgc cgagtcccga aacggctcgc accggatggg ccccgaggac
     4381 ccaattcatg acgtccaggt tgtgaacgtg ctgctccaca atatggtcgc ccgacaacca
     4441 gcagaaataa ttccaattgc ggagttgcca ttccatgtcg gaccaatggg gttctctttc
     4501 cacaacccag atttcacccc cattccaata gcacttcgca taaatgatct cgccaatcgc
     4561 cccatcttga atccgtttaa tcgtttcttg gtacctccgc tcatgccgtc tttgcgttcc
     4621 tgcagcaatg ccaaggttct tctgcttagc gatttggccg gcttggagga cagtcttgac
     4681 gccagggaca tcgacggcaa cgggtttctc cataaaaaca tgctttcccg cgttcaccgc
     4741 ggccatcagg tgctccgggc gaaaatgtgg tggagt
//
LOCUS       DAOPTR010000146         4776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_14441_length_4776_cov_3.111205, whole genome shotgun sequence.
ACCESSION   DAOPTR010000146 DAOPTR010000000
VERSION     DAOPTR010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14441_length_4776_cov_3.111205"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="PKI05_04085"
     CDS             <1..933
                     /locus_tag="PKI05_04085"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOA51409.1"
                     /translation="SPQQVVISNGAKHALHNAFTATLNPGDEVIIPAPYWVSYSELIK
                     LTGAVPVIVKTEESSGFKLNAAQLRQALTPRTRMLLLCTPSNPTGTFYTKSELEGIAD
                     VVLDADLTVVADEIYDQLIYTAEPFTSFPTVRPGLQERTILINGVSKTYAMTGWRIGW
                     AIAPTLEVAKAMGDLQSQETSNPCSISQYAALAALTGPQDCVETMRREFERRRDYVAQ
                     RIAKLPGVTAAPMQGAFYAFINISQHLGKRYGGTLVENSSEWCLELLAQKGVATVMGS
                     AFGAEGYARLSFATSMETLAAAFDQIEAFLQEAK"
     gene            complement(1027..2571)
                     /locus_tag="PKI05_04090"
     CDS             complement(1027..2571)
                     /locus_tag="PKI05_04090"
                     /inference="COORDINATES: protein motif:HMM:NF013715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HOA51410.1"
                     /translation="MTQSNAPAEEKGLPMTAKWDPEKLAREEAFLQELEKKSLWERIR
                     GYAKLTGPAWLQSAMTLGAGSAAASVMSGASFGYKLLWVQPLAMFCGVIVFAALAKIV
                     LTTGERPYRSFGRELHPIVPIFWALGTIVASIIWHFPQYTLAGAAAWDLAQVVQTNVF
                     DQKQAEGPGQQTEPGDKPQQGQNTTQMRGLELLVKYGAAACILMINIFTVWNYGSHAK
                     GIKLYEGFLRWVIRIVILAFLAVVIFTGINLKELFWGLFGFYIPETTEGITVVLGALG
                     AAVGINMTFLYPYSILAKGWGKHHRGLAKWDLFSSLLVPYTIVTSLIIIAMANTVYDG
                     KDAVRQGLQPVEAAAAFSTLMHSDLGRMILDLGFLAMTCGAISTHMVVCGFTVCEMLG
                     LQYTRARFRLFSLTPVIGILGAGMKLPFWLPILASAICLTMLPIVYLSIMIMANNRKY
                     IGDAVGSGFTRWVFNFLLLVAITVTTIGTVVQIKLKVVDPVRKWLNPPAAVQSATPSQ
                     AGEEKK"
     gene            2919..4505
                     /locus_tag="PKI05_04095"
     CDS             2919..4505
                     /locus_tag="PKI05_04095"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOA51411.1"
                     /translation="MAPHPAIIRVACPTVYTLSVLLWLGVGGNITSGQSIFDALPHLG
                     QGKTVAENGLWIETPLDRQFRQSKEVTIAEVQGPGVITMIHFALPQRMVAQPDKYRLG
                     RELAIRAFWDNETTPSVECPLVDFFCDPMGERESLDTLMVNKKRGWNAYFPMPFRKSA
                     KLTLSYDGPVEPGQELWELMPCYSYVMVRKVEELPANVGYFHAQWRQQLINLGKEDYV
                     SLDARGKGKFVGWNVTIRLPGQPGYPVDENEKFYVDGEETPSIEFQGLEDSFGFSWGF
                     PPEESTFLRTGWFPFHKDGAAAYRFFLEDSISFDRSLKVTIGFGANEHPTFRSRFGKP
                     GNELELSTTVYWYQSEPHAPFPALSPAEQRRPTERSWKDMENLPTSEALQSRGVKLHF
                     RCGRPENETIFAVPWYAAEVKQGFAYTGWPFPVFHTRAHEKEVQISLTVPTGRTGILR
                     LYMIDPDHFMGGRHQEVFIGDTSFGEIRDFDDGRWLEIPVDASMTSAGSLLVRIVNRN
                     PRSNAVVSILEWVEAAASNR"
     gene            complement(4548..>4776)
                     /locus_tag="PKI05_04100"
     CDS             complement(4548..>4776)
                     /locus_tag="PKI05_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51412.1"
                     /translation="WWRIKRSGEAAILWAPHWDRISQLVKEGKLPGKQVSENVLILDE
                     LDPRHYEVLTSESSVVADWENPVMLIRAKKD"
BASE COUNT         1131 a         1308 c         1239 g         1098 t
ORIGIN      
        1 tcgcctcaac aagttgttat ctcaaacggg gcgaagcatg cgctccacaa tgcctttacg
       61 gcaaccctca atccgggtga cgaagtcatc attccggcac cctattgggt gagctattcg
      121 gaattgatca agctcaccgg ggccgtgccc gttattgtga aaaccgagga atcttccgga
      181 ttcaagttaa atgccgctca attacggcag gccttgacac cacgaacacg aatgcttctt
      241 ctgtgcacgc cttccaatcc cacaggaacg ttttacacaa agagcgagct cgaaggcatt
      301 gccgacgtcg tcttggatgc tgatctaacc gtcgtcgccg acgaaattta cgaccaactt
      361 atttacactg ctgaaccgtt taccagcttt cccacggtcc gtccgggact gcaagagcgc
      421 actattctca ttaatggggt gagcaagacc tacgctatga cggggtggcg aattggttgg
      481 gccattgccc ctactctcga agtggccaag gccatgggcg acctacagag ccaagagacc
      541 tccaaccctt gcagcataag ccagtatgcc gccctggccg ccctgactgg cccccaggat
      601 tgtgtcgaaa cgatgcggcg tgagtttgaa cggcgtcgtg attatgtcgc gcaacgaatc
      661 gcaaagttgc cgggcgtcac ggccgcgccc atgcaggggg ctttctatgc ctttatcaac
      721 atttcgcaac acctgggcaa gcgctatggg gggacgttag tcgagaattc aagcgaatgg
      781 tgtctcgagt tactcgccca aaagggcgtt gccactgtta tgggctccgc ctttggtgcg
      841 gaaggctatg cgcgactctc ctttgccacc tcaatggaga ctctcgcagc cgcatttgac
      901 cagatcgaag ccttcctgca ggaagcgaaa tgacgcctag gcgcaaacca acccccgacc
      961 cagggatttc gtctggagtc gggggttgcc tcatttctca atgaatcagc gcaaggtaag
     1021 ggttgtctat ttcttttcct cgccggcctg acttggggtg gccgattgca cagccgccgg
     1081 tgggttcagc cacttccgga caggatcaac cactttcagc ttgatctgga caactgttcc
     1141 aatcgtcgtg acggtaatcg ccacaagaag caaaaagttg aagacccacc gggtgaatcc
     1201 cgaccccacc gcatcgccga tgtacttcct gttgttggcc atgatcatga tagacaagta
     1261 aacgatgggc agcatcgtta agcagatggc cgatgcaaga atcggcaacc aaaacggaag
     1321 tttcatcccg gccccaagaa tgccgattac cggtgtcaac gagaaaagcc gaaaccgggc
     1381 acgcgtatac tgcaagccca gcatttcaca aacggtgaac ccacagacaa ccatatgggt
     1441 actgatggca ccgcatgtca tggccaagaa accgagatcc agaatcatac gtcccagatc
     1501 cgaatgcata agcgtactga aggctgcagc ggcttcgacg ggttgaagcc cctggcgaac
     1561 cgcgtcctta ccgtcgtaaa ccgtgttggc catcgcgatg atgatgaggg acgtcacaat
     1621 tgtgtaaggg acaagcaatg agctaaaaag atcccatttg gccaaaccgc ggtggtgctt
     1681 cccccaacct ttggccagga tggaatacgg atagagaaac gtcatattga ttcccacggc
     1741 ggcccccaac gcacctagga caacggtgat cccttccgtc gtttcgggga tgtaaaaccc
     1801 aaacagcccc cagaatagtt ccttgaggtt aatgccggta aaaatcacca cagccagaaa
     1861 agcgagaatc acaattcgta tcacccaccg caggaatccc tcgtagagct tgatcccctt
     1921 ggcatgactt ccatagttcc aaacggtgaa aatgttgatc atcaggatgc atgcggcggc
     1981 cccatacttg acgagcaatt caaggcctcg catttgggtt gtattctgac cctgctgcgg
     2041 cttgtcgcct ggctcggttt gttgccccgg gccctcggcc tgcttttgat caaacacgtt
     2101 cgtttgcacg acttgcgcga ggtcccacgc ggcggcacct gcgagtgtgt actgcggaaa
     2161 gtgccaaatg atggatgcaa cgatggttcc aagagcccaa aagatcggca cgatgggatg
     2221 caattcccgg ccaaatgatc gatagggtct ttcgcccgtc gttaggacga tctttgccag
     2281 cgctgcgaaa acgatgacac cgcaaaacat ggcgagcggt tggacccaga ggagcttgta
     2341 accgaaagat gcacccgaca tgacagaggc ggctgcacta ccggctccca aggtcatggc
     2401 actttgcagc caggcaggac ccgtgagctt cgcatatcca cggatccgct cccaaaggct
     2461 tttcttttcc aactcttgaa gaaatgcctc ttcccgagcc aacttctcgg gatcccattt
     2521 ggccgtcatg ggaaggcctt tttcttccgc aggtgcattt gactgagtca taactgtctc
     2581 cctcgcgcaa gtatgttttt caggacgccg agccgccaga aaacttccct tcaagaacga
     2641 actgcgagtt agaggtgttc gccaccacct cacggtacca atgacagtct ctcgtcgcca
     2701 tcgcaaaacc ggatagacca aggttagtct cgacagattc acatttcaag gttacttcgg
     2761 ggaatttcgc catgtacttc ttgtgaaatg gattgatgtg tattaggctg gttgcacgtc
     2821 caggcaatag ccgagcgaca gcgtactgtg gcgagttgcg tttcggaatg ttgttttcac
     2881 acctgaatct tgtccgccag aaacggaagg gaagagacat ggcaccgcac cccgctatta
     2941 tcagggttgc ctgtcctacg gtgtacacgc tgagcgtgct tctgtggtta ggcgtcggtg
     3001 gcaacataac gagcggtcaa tccatctttg acgctttacc gcatctaggg caaggaaaaa
     3061 ctgtagcaga aaatggcctt tggatcgaaa cgcctttaga tcggcaattc cgccaatcga
     3121 aggaggtcac aattgccgag gtccaggggc ccggcgtgat caccatgatc cacttcgccc
     3181 ttccccaacg catggtggcg cagccggaca aatatcggct aggacgtgag ttggccatcc
     3241 gcgcgttttg ggataatgag acaacgccga gtgttgagtg cccactcgtt gatttcttct
     3301 gcgatcccat gggagagagg gaaagtctcg atacgttaat ggtcaacaaa aaacgcggtt
     3361 ggaatgccta ctttcccatg ccgtttcgca agtcggcgaa attgacactt tcttacgacg
     3421 gccctgtaga gcctggacag gagctttggg aactgatgcc ttgttatagc tatgtgatgg
     3481 tccgtaaggt cgaagaattg cctgcgaatg tcggttattt tcatgcgcaa tggcgacagc
     3541 aactcatcaa tctcggcaaa gaagattacg tgtccctcga tgctcgcggg aaaggcaaat
     3601 ttgtgggatg gaatgtcacg attcgccttc ccggccaacc cggttacccg gtggacgaaa
     3661 atgaaaagtt ctacgtagat ggtgaggaaa caccctcaat tgaatttcaa ggattggaag
     3721 attcctttgg cttcagttgg ggatttccgc cagaggaaag taccttcttg cggacagggt
     3781 ggtttccttt tcacaaggac ggggcggcag cttaccgctt ctttttggag gactccattt
     3841 ctttcgaccg ctcgcttaag gtaacaatcg gtttcggcgc aaacgagcac ccaacgtttc
     3901 gatctcgatt tggaaaaccc ggtaacgaac tagagttatc aacgactgtt tactggtatc
     3961 agtcagaacc ccatgccccc ttccctgccc tatccccggc ggaacaacgt cgcccaacag
     4021 aacgatcgtg gaaagacatg gagaatctgc cgacgagcga ggcactccag agtcgcggcg
     4081 ttaagttgca ctttcgctgt ggacgtcccg aaaatgaaac gatttttgca gtgccatggt
     4141 atgcggcgga ggtcaaacaa ggcttcgctt acacgggttg gcctttcccc gtgtttcata
     4201 cacgtgccca tgaaaaggag gtccaaatct cacttactgt gcccacagga agaaccggca
     4261 tcctgcggct ttacatgatt gatcccgacc acttcatggg cggtcgtcac caagaggtct
     4321 ttattggcga cacctcgttt ggcgaaatca gggattttga tgacgggcgt tggctcgaga
     4381 ttccggttga cgcgtccatg acctcggcag gctctctctt ggttcgcatc gtcaaccgaa
     4441 atcctcgctc gaatgccgtg gtatccattc ttgaatgggt cgaagccgcg gcatcaaacc
     4501 gttgattccg tggtttctgg aatcgcaatt agtgtccctg ccaatcgtta atcttttttc
     4561 gccctaataa gcatgaccgg attttcccag tccgccacaa cgctgctttc tgaagtgagt
     4621 acttcatagt gccttggatc aagctcatct aggataagca cattctcact gacctgtttt
     4681 cccggcagct tgccttcctt gacgagttga gaaattcgat cccaatgagg agcccataaa
     4741 atggccgcct ctcctgagcg tttaatccgc caccag
//
LOCUS       DAOPTR010000147         4773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_14460_length_4773_cov_3.444892, whole genome shotgun sequence.
ACCESSION   DAOPTR010000147 DAOPTR010000000
VERSION     DAOPTR010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14460_length_4773_cov_3.444892"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..1400
                     /locus_tag="PKI05_04105"
     CDS             27..1400
                     /locus_tag="PKI05_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOA51413.1"
                     /translation="MSSSQPGTTRRGFLKTTGQIAAASALTGVAMPHVHAGENNTIQI
                     ALIGCGGRGTGAASDALSVQKRLNVPVKLVALADVFADRVERSFKSLKERFADQVDVP
                     EDRLFVGFDAYRKAMDCLRPGDVAIFATPPAFRWVFFQYAIEKNLNVFMEKPVTVDGP
                     TSRRMFELGKKSVEKNLKVGVGLMVRHCRGRQDLKKRIEDGEIGEIQFLHAYRMGGPA
                     ANIGPKPDNISELRYQIRQFHSFIWASGGVYSDFYIHQIDECSWMKGAWPIKAHALGG
                     RHYRGKAVDQNFDTYSIEYTYPDGTKLFYFGRQMAGCHNEFASYGHGTKGCCIISTSA
                     HTPGRVRTFKGHDFAQENLLWAFPQPEPNPYQMEWVDLIEAIIKDQPYNEVERGVQAS
                     LVTSMGRMAAHFGRIVTYEEALNHPHELAPDVDKFTEDSPAPVQFDETIGMYPQPMPG
                     IKTDREY"
     gene            complement(2062..3456)
                     /locus_tag="PKI05_04110"
     CDS             complement(2062..3456)
                     /locus_tag="PKI05_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51414.1"
                     /translation="MSSQGVPSLGATGTTARIDPPHRVGQTQAPQIATSAPRQDAGEP
                     GPVVVNEEIPISEHEEQDVERGVGLSPERIRRHARQIAAVLRRRQEQLDHREAQLNAQ
                     RAQLENDIRVLRLLWQERHAEWEEKRQLLAEREAALAERRAELGEREEALSQREQFLA
                     QWEEALRQREANLAKEEQALQEKRQELARREAEVQIRESDLTTFAVLVETAPAPPSVE
                     RSEGVPCERQADGVEDLIRKQDELTHYWQQAKRILSELEDARERWKEQEKQWRQQWVA
                     EQRQAMAELAAQREAVARRSQALDRLHAELLQLKKEVDARQREAIEHHLAACELTASL
                     ERNHAPAEVAQRLIALRQKLDQTVKMALEEREDRHQQTRTIETRLQSLHRQIVEEKRR
                     FDKYMAARLKELEDLAAKLADREKQLVLKESELHSQATAWQLAELGYILEIRRLRECL
                     QPDSQFGDLPESTT"
     gene            3910..>4773
                     /locus_tag="PKI05_04115"
     CDS             3910..>4773
                     /locus_tag="PKI05_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOA51415.1"
                     /translation="MVTEAFITLVFSRPRDPHAEPAFRHMVNEVLEAAGHLPAVIIPH
                     FYDAMPGGPVFTYLRRLTTDIILLAPLYPRAAQLVLRANGLRYRLARQTDDVPQRQNQ
                     SDRRLWAFDYREYGSFEVLRNAVAALVAEYLQGAGSLAGERPLVEEIEEATRSRWYPV
                     IDDEACVNCFECLNFCLFGVFDVNTEGRVFTAQPDQCRPGCPACARVCPAGAIVFPLY
                     PDPKIAGGDVVEEGQIIPLVDLKSGIGTVTTPQKPSVAGNSSQAPRPTGDRADDKESH
                     PPTKQTLQQWLC"
BASE COUNT          951 a         1357 c         1340 g         1125 t
ORIGIN      
        1 ttttcgtctg actagggagg ttcgttatga gttcgtccca accgggaaca acccgtcgtg
       61 gtttcctcaa gactacgggt cagatcgcag cagcgtcggc gctgacgggc gtcgccatgc
      121 cgcatgtgca tgcaggtgaa aataacacga tccagatcgc cctcattgga tgtggtgggc
      181 gaggcacggg ggccgcgtcc gacgccctgt ctgtgcaaaa gagactcaac gttcccgtta
      241 aacttgtggc cctagcggat gtatttgccg accgcgtcga gaggagcttc aagagcctga
      301 aggaacgatt tgccgatcaa gtcgatgtgc cagaggaccg tcttttcgtc ggcttcgacg
      361 cgtacaggaa ggccatggat tgccttcgtc cgggcgatgt cgccattttt gcgaccccgc
      421 cggccttccg ttgggtgttc ttccaatatg caatcgaaaa gaacctcaac gtgttcatgg
      481 aaaaaccggt cactgtggat ggccccacgt cacggcgaat gttcgaacta ggcaagaagt
      541 ccgtagaaaa gaacctcaag gtcggtgtgg gactgatggt ccgacattgc cggggtcgtc
      601 aggatttgaa gaagcggatt gaagatggcg agatcggtga aattcaattt ctccacgctt
      661 accgaatggg tggccccgcg gcaaacattg gaccaaaacc tgacaatatc agtgaattgc
      721 gctatcagat tcggcaattt cacagtttca tttgggccag cggtggtgtg tacagcgact
      781 tctacatcca ccagatcgat gagtgttcct ggatgaaggg ggcttggccg atcaaggcgc
      841 atgccttggg tggacgacac tatcgcggga aggcggtgga ccagaacttt gatacgtatt
      901 ccattgaata cacctatcct gacggcacca agttgttcta ctttgggcgg cagatggctg
      961 gttgccacaa cgagttcgcc agctacggtc acgggacgaa aggctgctgc atcatttcca
     1021 cttcagccca tacccccgga cgggtccgca catttaaggg tcacgatttt gcccaggaga
     1081 atctcctctg ggctttcccc caacccgagc ccaaccctta tcaaatggag tgggtcgatc
     1141 tgattgaggc catcatcaag gatcaaccct acaatgaagt ggaacgcggt gttcaggcaa
     1201 gcttggttac gagcatgggc cgcatggctg cccactttgg ccggatcgtg acctacgagg
     1261 aagcgctgaa ccatccgcac gagctggccc ctgatgtcga taagttcacc gaggactcgc
     1321 cggcaccagt tcagtttgat gagacgatcg gtatgtatcc ccaaccgatg cctggcatca
     1381 agaccgatcg ggagtattga tcctcacgga tcttggcaag acccggcggt ggaacacgat
     1441 cgcctttaaa aaagcggttt taccgccagc aacccttgaa gattgttcta ccagacgaat
     1501 ccttagctag aacggaaggc ccatagcggt tatccgctgt gggccttcgc tttgcgctaa
     1561 gaaactttgg gtgcgctgga tttggagaga cgcgcgtcca atgtggtgcc gacggagatc
     1621 ctatcatgtc acgcggccac gacggcgtga agccctcgaa tgaatcgggt gggcacacgt
     1681 cctattcttg cccggtgggt cggagcgcct accacggatc ggcccccact ttacggtgcg
     1741 ggttgtcgct ttccgattca gcaatgtatt gcggccgcgc tgtaaagatt ggtaggggcc
     1801 aacgttgtga ttgtccgttg ttgagtctca ggagcgacac gcggcgcgtt gtgttaagag
     1861 cccgtcccat aatctcccct cgcccgctcg gcgggagagg ggggcttttt gagataggct
     1921 ctaacccaaa acgacgcgca ccattgcggc cgcgggcgtg tgatactcat cctaacaacg
     1981 atttaccacg atgagaaaca ctcaagatgc cttgtgtgat acgacgagaa gcttgttcca
     2041 acccgttata cgaccaggaa actatgtagt actctctggc aaatcaccaa attgggagtc
     2101 aggttgtaag cactcgcgga gacgccggat ttccaagatg tatcccaact ccgcgagttg
     2161 ccacgctgta gcctggctat gaagctcgct ttccttgagg acgagttgct tttctcggtc
     2221 ggcaagtttg gccgcgaggt cttctagctc cttaagccgg gccgccatgt atttgtcaaa
     2281 gcgccgtttt tcttccacaa tttggcgatg cagggattga agacgggtct caatcgttcg
     2341 agtttgctga tgccgatctt cccgttcctc caatgccatc ttaacagtct gatccagttt
     2401 ttgccgcagg gcgattaacc gctgggcaac ttcggcgggc gcgtgattcc gttccaagga
     2461 cgccgtaagc tcacaagccg ctaggtggtg ttcaatggcc tcgcgctgcc gggcatccac
     2521 ttcctttttg agttgcaata actcggcgtg gagccgatcg agggcctgtg aacgccgcgc
     2581 gacggcttcg cgctgggccg cgagttccgc catagcctga cgttgctccg ctacccattg
     2641 ctgacgccac tgtttttctt gctctttcca tcgctctctg gcatcctcca actccgacag
     2701 gattctcttg gcttgctgcc agtagtgcgt cagttcatcc tgctttcgga taaggtcttc
     2761 gaccccgtcg gcctgacgtt cacacggtac accttcgctg cgctccaccg agggtggggc
     2821 tggcgcagtc tccaccaaca cggcgaacgt cgttaagtcg ctttctcgga tttggacttc
     2881 ggcctcgcgg cgggccaatt cctgccgttt ttcctggagg gcctgctcct cctttgcgag
     2941 attggcttcg cgctggcgaa gtgcctcttc ccattgtgcg aggaactgct cgcgctgcga
     3001 cagggcttcc tcgcgttcac cgagttcagc gcggcgctcc gcaagggcgg cctctcgctc
     3061 ggccaacaac tgccgttttt cctcccactc ggcatggcgt tcctgccaaa ggagtcgcag
     3121 gacacgaata tcgttctcaa gctgtgcccg ctgcgcgttg agttgcgctt cccggtggtc
     3181 aagctgctct tgacgccgcc ggagaaccgc cgctatttga cgcgcatgcc ggcggattcg
     3241 ctccggcgaa agtcccaccc cccgttcaac gtcttgctcc tcatgttcac tgattgggat
     3301 ctcttcatta acgacaacag gtccgggctc gccagcgtct tgcctcggcg ccgatgtcgc
     3361 aatctgtgga gcttgggtct gaccaactcg gtgcggtggg tcgatccgtg cagttgtccc
     3421 ggttgcccca agggacggca cgccttgcga tgacattgca tcgcatgccg gggccatctg
     3481 tgggtgactc agcggatcgg acattgtcgg gagactaaag attcctgctc tcggacgtca
     3541 agctccatca ccaccgtggc gacgagcatt gaccgcgacg accaagttca aaggagcctc
     3601 gccgcagcct ttatcgatat aatcgacaaa gtttactcat ttcttgtatt ccgtttcttt
     3661 gcggggcaaa tcgtgctccg cgaacgctgc ctcaaagctg tccgcgctac ccatcctgcc
     3721 aaaaagcgga gatattaccc attttgatga gctgtcaaga atgaccaggt caacaatgat
     3781 caaccagacc agtttttcca gattaccccg attctcggca gggatcgcag tagccctttg
     3841 gaagatcgac ggtcctctcg caatttgcta tactgcgaat aactgattcg aatttcgtaa
     3901 ggtgtgatta tggttactga ggcattcatc acattggtgt tttctcgccc aagggatccg
     3961 catgcggagc cggcgttccg gcatatggtc aacgaggtcc ttgaggcagc cggccacttg
     4021 cccgcagtga tcattcccca tttctatgat gcgatgccag gtggcccggt gtttacatac
     4081 ctccgccgac tgacaacgga catcatcttg ctcgccccgc tgtatccccg ggcagcgcag
     4141 cttgtgttgc gtgccaacgg tctgcggtat cgcctggccc gtcaaaccga cgacgtgccg
     4201 cagcgacaaa atcaatctga tcgtcggctt tgggccttcg actaccggga atatggctct
     4261 ttcgaagttc ttcggaacgc cgtggcggca ctggttgccg aatatctcca gggtgcgggg
     4321 tcccttgctg gcgagcgacc gctagtggag gaaatcgagg aagctactcg ctcacgttgg
     4381 tatccggtca ttgacgacga ggcatgcgtc aactgttttg agtgtctcaa tttctgtctt
     4441 ttcggtgttt tcgatgtcaa tacggaaggt cgcgtgttta ccgctcaacc ggatcaatgt
     4501 cgtcccggtt gcccagcctg tgcccgcgtt tgtcccgccg gggccattgt ttttccactt
     4561 tatccggatc ccaagattgc tggcggggat gttgtcgagg agggacaaat catccctcta
     4621 gtggatctga agagcggaat tgggacagtg acgaccccgc aaaagccatc ggtggcaggc
     4681 aattcctcac aagccccgag gccgacaggt gatcgggcgg atgataaaga gtctcatccc
     4741 cctacgaagc aaacgctgca gcagtggctt tgc
//
LOCUS       DAOPTR010000148         4758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_14522_length_4758_cov_2.834574, whole genome shotgun sequence.
ACCESSION   DAOPTR010000148 DAOPTR010000000
VERSION     DAOPTR010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14522_length_4758_cov_2.834574"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="PKI05_04120"
     CDS             complement(<1..1322)
                     /locus_tag="PKI05_04120"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOA51416.1"
                     /translation="MNQDHPSSSSEQGQRPKSHLEAARREKLRRIAEMGIDPWGGRFD
                     GHRPIREIRARENEIVVVDGGDGRSAPELRGPRVRAAGRIMLLRRAGKIMFIDIRDWT
                     GKIQVLVGKAQVGERNWELAQCFDLGDIIGVDGELKRTKTGELTIFADNLHFLTKSIE
                     TPPEKWHGLADPELRNRMRYLDLAYTEGVMERFLRRTQIVHSIRATLAEEGFVEVEGP
                     TLHVIAGGAAARPFVTHHNALDMDLYLRIALELHLKRLLVGGMEKVYELGRVYRNEGI
                     SPRHNPEFTMLEAYQAYGDYHTMMDLTEKLIVQAIRATGQPLQLPWGENVIDFTPPFA
                     RKTYDELFAEHTGVNPTDPASISYFASSRGIEVVGRHPDVIKNEIFEEFVQPKLTGPI
                     FVLDYPASICPLTKRSRNNPQIAERFELFVQGMELANAYTELNDPDLQ"
     gene            1723..2808
                     /locus_tag="PKI05_04125"
     CDS             1723..2808
                     /locus_tag="PKI05_04125"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOA51417.1"
                     /translation="MSGINPLRVAVIGGDGTGPEVIAEAVKVLKAVAQMEGITLQLQE
                     FDLGGERYLRTKEILPENVFEQLKTFPAILLGAIGHPDVPPGILERGLLLELRFRFDQ
                     YINLRPVKLYPGVETPLAGKRPEDIDFVVVRENTEDLYAGIGGFLKKGTPDEVAIQTA
                     IYTRKGCERCIRWAFEYTRKRNKKKRLTLVAKTNVLTYGHDLWWRTFQEVAKEYPDIQ
                     TDYNHVDACCMWMVKNPEFYDVIVTTNMFGDIITDLAAIIQGGMGVAAGANINPEPGG
                     VSMFEPMGGSAPKYTGKGMINPIAAIAAAGMLLEHTGHERAAERVHQAIQVVTGTKMK
                     SQAAGKMGYTTSQVGDLVVEALSQVNV"
     gene            2962..3636
                     /gene="cysC"
                     /locus_tag="PKI05_04130"
     CDS             2962..3636
                     /gene="cysC"
                     /locus_tag="PKI05_04130"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HOA51418.1"
                     /translation="MSGCDPKVFWHEHAVSREERERLNGHKGCVLWFTGLSGSGKSTL
                     ANLVDHRLHAMGVHSFVLDGDNIRHGLNASPAMLKERHGEEFAKRFGLGFSAQDREEN
                     IRRIGAVAKLFCEAGIIAITAFISPYRIDRDRVRATMKPGDFIEIFVDAPLEVCEARD
                     PKGLYKKARAGELKGFTGIDDPYEPPLRPEIHLDAANHSPEELADVVINYLREKGIIG
                     ATPGGK"
     gene            4117..4365
                     /locus_tag="PKI05_04135"
     CDS             4117..4365
                     /locus_tag="PKI05_04135"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOA51419.1"
                     /translation="MVVDVLSGNLQARAESALRASPIHELRDVNVEQQGDTIILTGVV
                     SSYYYKQLAQEAVRSVLRNHNVEVLNQIEVRPYSFDRD"
     gene            4488..>4758
                     /locus_tag="PKI05_04140"
     CDS             4488..>4758
                     /locus_tag="PKI05_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOA51420.1"
                     /translation="MSFYSPFDEDVVVREGHLDEYPLSPAQAEAERLATQILRAVEAA
                     TAGAVADLRVTIDGDCVILSGRCPSYHIKQKAQHAAMPFAPGVLQN"
BASE COUNT         1051 a         1268 c         1404 g         1035 t
ORIGIN      
        1 tcctgcaaat cggggtcgtt gagctctgtg taggcgttgg ccaactccat cccttggaca
       61 aagagctcaa accgctcggc gatttgagga ttgtttctgc tcctcttggt tagagggcaa
      121 atcgaagccg gataatcgag aacaaaaatc ggtcctgtca atttcggctg aacaaattct
      181 tcgaagatct cattcttgat cacgtccggg tggcgaccta ccacttcaat tccgcgggag
      241 gaggcgaaat aggagattga ggcaggatcg gtaggattaa cccccgtgtg ctcagcaaac
      301 aactcatcgt atgttttccg tgcaaagggt ggtgtaaaat cgatgacatt ctctccccac
      361 ggaagttgca acggctgtcc cgtggccctg atcgcctgga cgatcagctt ctcggtcaaa
      421 tccatcatcg tgtggtaatc gccgtacgcc tggtaggcct caagcatggt gaactcgggg
      481 ttgtgacgcg ggctgatccc ctcattgcga tagactcgac ccaactcata gaccttctcc
      541 atgcccccga cgaggagccg tttgagatgg agctccaagg cgatgcggag gtaaaggtcc
      601 atatccaagg cgttatggtg ggtaacaaag ggacgcgccg ctgccccccc ggcgatcacg
      661 tgcagggtgg ggccttccac ttcgacaaag ccttcctcgg ccaacgtcgc gcggatggaa
      721 tgaacaatct gcgtccgtcg caaaaagcgc tccatgaccc cttcggtgta agccaagtcc
      781 aggtatcgca tgcggttacg gagttccggg tcggccaacc catgccattt ctccggcggc
      841 gtttcgatcg actttgtcaa aaagtggaga ttgtcggcga aaatggttag ctcaccggtc
      901 tttgtccgtt tcagttcgcc atcgacaccg ataatgtctc ccaggtcaaa gcattgggcc
      961 agttcccagt tccgctcccc gacctgcgcc ttgccgacga gcacctgaat ctttccagtc
     1021 caatcgcgga tatcgatgaa catgatcttg ccagcacgac gcaggagcat aatccgcccg
     1081 gcagcgcgga cccggggccc ccgcagttcc ggcgcagatc gaccatcgcc gccgtcaacc
     1141 acgacgatct cattttcccg cgcgcgaatt tccctgattg gccgatgacc atcgaaccgc
     1201 cctccccatg ggtcgatccc catctcggca atacggcgga gcttctcccg gcgggccgcc
     1261 tcaagatgac tcttgggccg ctgaccctgt tctgaagacg acgacggatg atcctggttc
     1321 atatgtttgg tttccttcgt ggatttgatc tcccgtggat ttgatctcgc catgggtgac
     1381 gccagaaatc aaaccgcgtc actggtgtgg gtaaatgtca caaacatgcc ggaaagcatc
     1441 tgttactcca acacaagaca ccgttgacca aatggcggtt ctgcttaacg atcccacccg
     1501 ttgggtgtca agtgtaacct tttcagttta cccggttatc agcaatcgcc aacggggaag
     1561 cttccacgat ggcgcgggac cctccgtcag gaggtcatcg gttggtgggg ggcaatcccc
     1621 ggtggttggc ccggcataca catttggtgg ccgacttttc gcgctctact attcggcgta
     1681 aaataacgct tttcctgtca agcaaagaag ggtgaagaga atatgagcgg tatcaatccc
     1741 ctgcgtgtgg ccgtcattgg tggcgatggc actggtccgg aggtgatcgc cgaggccgtc
     1801 aaggtcctca aggccgtagc ccagatggaa ggaatcacgc tccagctcca agagttcgac
     1861 ttgggtggcg agcgctatct gcggacgaaa gagatcctgc cggaaaacgt cttcgaacaa
     1921 ctgaagacct ttcccgccat cttactaggg gcaattgggc atcctgatgt gccgccgggt
     1981 attctggaac gtgggctcct gctggaactg cgattccgct tcgatcaata catcaacctt
     2041 cgaccggtga agctctatcc cggtgtggaa acacccctgg caggcaaacg acctgaggat
     2101 atcgacttcg tggttgttcg cgaaaacacg gaagacctct acgcgggcat cgggggcttc
     2161 ttaaaaaagg gtaccccgga cgaggttgcc attcagacgg ccatttacac ccgcaaaggc
     2221 tgcgaacggt gtatccgctg ggcctttgaa tacacccgta aacggaacaa gaaaaagcgg
     2281 ctgactctcg tggctaagac gaacgtcttg acctatggtc acgacctctg gtggcgaaca
     2341 ttccaggaag tggccaagga gtatcccgac attcagacgg attataacca cgtcgatgcc
     2401 tgctgcatgt ggatggtgaa aaaccccgag ttctacgacg tgattgtgac gaccaacatg
     2461 ttcggggaca tcatcaccga tttggcggcg atcatccaag gcggcatggg cgtggctgcg
     2521 ggggcgaata tcaatcccga acccgggggt gttagcatgt ttgagccaat gggcggaagt
     2581 gctcccaaat acacggggaa agggatgatc aaccccatcg cggcgattgc cgcggcgggt
     2641 atgctcctgg aacacacggg ccatgagcgg gcggcggaac gtgtgcacca ggccatccag
     2701 gtggtcaccg ggaccaagat gaaaagccag gccgccggaa aaatgggcta cacaacctcc
     2761 caggtgggcg accttgtggt ggaagccctc tcgcaggtga atgtctgacc gaaagcgggt
     2821 ggctcaaccg gtcataagat cactgaggac ctgccaagcg tctggttgtc gctcatcgaa
     2881 gaccaatggt cggggtttat caccgttggg gtaaaccatc gcgatgaaac aatttagtca
     2941 atatgcataa gagagtaaaa gatgagtggc tgcgacccaa aggttttctg gcatgaacac
     3001 gcggtctccc gggaagagcg ggagaggctg aatgggcata aagggtgtgt cttgtggttc
     3061 acggggctaa gcggctccgg caagagtacg ctcgccaatc tggtggatca tcggctgcac
     3121 gcgatgggag ttcatagttt tgtcctcgat ggggacaaca tccgccatgg cttgaacgcc
     3181 tcgcctgcca tgttgaagga acgtcatggg gaagagttcg caaaacgttt cggcttaggc
     3241 ttttctgccc aagatcggga ggaaaatatc cggcggattg gggccgtcgc caagcttttc
     3301 tgcgaggccg gaatcattgc catcacagcc tttattagcc cctaccggat tgatcgagat
     3361 cgggttcggg ccacgatgaa accgggcgat ttcatcgaaa tctttgtgga tgccccccta
     3421 gaggtgtgcg aagctcgcga cccgaagggc ctttacaaaa aggctcgggc aggcgagctt
     3481 aagggcttca ccgggattga cgatccctat gaaccgccgc tccggccgga aatccacctc
     3541 gatgcggcaa accatagccc agaagagctg gcagacgttg ttatcaatta tctccgagaa
     3601 aagggcatca tcggcgccac gcctggcggc aagtaaggtt tccaaagctg atcaccctac
     3661 tcgccaatca aggaatataa gccctttgga gggggtgaag taacaagaag gagttatcca
     3721 tccccgcttg attccctaat tcgtttgggc gaagtgtccc ttgggaacct gggaacgggc
     3781 gagcatcatg ctggggtgcg ggtggggcgt gaaagctgtg gcggggccac aactaaggct
     3841 aagaatcgcc gactctgggg ttgactcttg cgtgtcgcct gatataaatg atcttggtcg
     3901 tcgcccagta gatcgagatg gaaacgcctg ggtgcgaccg caatttgatc cgcattttag
     3961 cccctgtgac gagcggtggg ttccgatttt agatgataag cccgctgcca gcatcttggt
     4021 tgataatggc ttctctgggt ttgcagcgaa agctgaccgg cgcgggccgg tgtgaggttt
     4081 ggttcattgg tcatggcacc agggatggaa gcagctatgg tggttgatgt gctttccggc
     4141 aaccttcagg cgcgcgcaga gtcggcgttg agagctagcc ccattcatga actgcgcgac
     4201 gtcaacgtcg agcaacaggg cgatacgatc attctgactg gagttgtctc cagttattat
     4261 tacaaacagt tagcgcagga ggcggtccgg tcggttttgc gaaaccacaa tgtggaggtt
     4321 ctcaatcaga tcgaagtgcg gccgtatagc ttcgatcgtg attgaaggtg tggggtttcc
     4381 taccgaccgg tgaaacgttt ctggggaatg agggaacagg gatggccctc ggtgttttgt
     4441 ggtgtggggt gtgaaaggat cgcatttccg cgcccgtgga gggaaccatg tccttctatt
     4501 cgccgtttga cgaggacgtc gttgttcgcg aggggcatct ggatgaatat ccattgtctc
     4561 ccgcgcaggc agaagccgaa cggctggcaa cgcagatcct gcgagcggtg gaagccgcca
     4621 cggccggtgc ggtggccgat ctgcgggtca ccatcgatgg tgactgcgta attctttcgg
     4681 gccgttgccc aagttatcac atcaagcaaa aggcccaaca tgcagccatg ccgtttgccc
     4741 caggtgtttt gcagaacg
//
LOCUS       DAOPTR010000149         4740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_14597_length_4740_cov_2.698826, whole genome shotgun sequence.
ACCESSION   DAOPTR010000149 DAOPTR010000000
VERSION     DAOPTR010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14597_length_4740_cov_2.698826"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..900
                     /locus_tag="PKI05_04145"
     CDS             256..900
                     /locus_tag="PKI05_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51421.1"
                     /translation="MRSYARTPRFGGESIGSTIGFFFGLSACRWRIYRWVPHPPRGGR
                     PLEFHCTTPVKPTRTQEILFGATLKPYLFGEQIGPVLFAKAKTPVHAIFVSQWESLTL
                     ASQISEPVAFVCPEGESPLLEGADGASDVPRSNGRWETSPSNETQTCGVIERVGLPPC
                     QVVRIGKNRLVVAAIRPEAPEELRELLSPWEAGLDLLEPFGRIQAALEEASRRG"
     gene            965..2914
                     /locus_tag="PKI05_04150"
     CDS             965..2914
                     /locus_tag="PKI05_04150"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOA51422.1"
                     /translation="MSHQALDRVPEGRPSASRVETPLPTATAIEIAKSWFPGDDFACD
                     AHSESLVFSVQIYPTGWNRAIQRGCESVVQSVDITCKPPRVLSLQIRDPVICVRRFFF
                     LADEQTEGSPSQQRCQNVSGSNAAQSRQQESQNDPQTPGRSRLRCCAEELLRLVTAGH
                     QHRIEVPEDVIKLEDRLRYLLQPSLEAILAERQLEFPFTPFPYQLEGVAFLFPRHAAI
                     LADEMGLGKTMQAITAIRLLLRCGEVRRVLLVCPKPLVSNWRREFELWAPEIPVMVIE
                     GDPQRRKWQWELADVPVRIANYELLPRDQEFFTASDETGQTKVAFDLVVLDESQRIKN
                     RHSATNQAVCSIARKRSWALTGTPVENSTDDLVGIFEFLSPGFLSPDMKPQRLGRTIA
                     DYVLRRTKDRVLSDLPPKILRDAEVELTPEQRETYDLAETAGVLRLTELGDAVTIQHV
                     FELVIRLKQICNFDPATGASAKMERLEADLEEIAGSGRKAIIFSQWVATLEEIGRRLE
                     RYKPLYFHGQTPVRQRDEVIRRFREDPQHRILLMSYGAGSVGLNLQFVNYVFLFDRWW
                     NPAVEDQAINRAHRIGAAGPVIVTRFLTVDTIEQRINMVLEQKRQLFETIFSGTVEPK
                     KLALTQEEIFGLFDLKTMATRAKAA"
     gene            complement(3390..4553)
                     /locus_tag="PKI05_04155"
     CDS             complement(3390..4553)
                     /locus_tag="PKI05_04155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA51423.1"
                     /translation="MEADLSVIITTFERPWHLRRVLLSLAIQRDCHCRLEVVVADDGS
                     RDETPQVVEEFARQVDFPVRFVTHPHEGFWPARARNEGFIASTGRYLLFLDGDCVIPP
                     DHLAAHMRMRRPHRAWTGDCIRLDQMMTQRMTEDAVKNADYLRWPLRAEYRRLAIAHW
                     KAVFYHAIRHPRKPRVLSGNLAVWRHDFLRINGFDEQYRGWGCEDDDLGLRLHQAGIR
                     VFSIRRLTRTYHLWHPRVESAPSKVSNGLNFAYLHRAGRLSCCRNGLKKRSWKDLHVV
                     WTGNPAAISDYCGAELCPPDGCPSSPFGGRTSDRPEVEILIWDWSARSFRTDAECRVL
                     VIIKGQTPPKGVVRASDWVLADGERLDTFRPWIKQGARLAPLERFREALEEIG"
BASE COUNT         1030 a         1312 c         1358 g         1040 t
ORIGIN      
        1 ccaattcaaa aatcagtata accagcacgg gcaggcaaag tcgagtgtcc gacacagaac
       61 tttaacaaaa gtttcatccg ataactgaaa agtttctcgt ttgttccgca caaccggtac
      121 agatccgcta cgtgctaatg tccgcaaagc cgtaaagttg cgccttattt ggttccgatg
      181 atgaagcaga tcatgtcggt aggagggacc atcgtccccg attgatctgt tttgctaacc
      241 ttgagctggc atctcttgag atcttatgcg cgaacaccac gattcggtgg ggaatcgatt
      301 ggctctacga ttgggttttt tttcggtctg tccgcatgtc gatggcggat ttatcggtgg
      361 gtacctcatc ctccacgggg tgggcgaccg cttgagttcc actgcaccac gcccgtcaaa
      421 ccgacacgaa cccaagaaat cctcttcggg gccacactga agccctacct ttttggggag
      481 cagattggcc cggtgctttt cgccaaagcc aaaacacctg tccatgcgat tttcgtctcg
      541 cagtgggaat cgttgacctt ggcgtcacag atttcggaac cagtagcctt cgtctgtccc
      601 gaaggcgaat cacctttact ggagggagca gatggggcgt cggatgtgcc acggtcgaac
      661 ggtcgatggg agacctctcc ctccaatgaa actcaaacgt gcggggtgat cgagcgagtg
      721 gggctccccc cttgccaagt ggtgcgaatt ggaaagaacc gtcttgtggt tgctgctatc
      781 cggcccgagg cgcctgagga gctcagggaa cttctcagcc cttgggaggc ggggcttgat
      841 ctcctcgaac catttggacg aatccaagcg gctctcgaag aggcttcacg tcgggggtga
      901 tcgcatgttc tggccgtggt ctttgcgaag gcaatcagag agccaaccga gggcaacgtc
      961 tgtcgtgtct caccaagcgc ttgacagggt gcccgaggga cggccaagcg catcccgagt
     1021 agaaacgccc ctgccaacag cgaccgccat cgaaattgcg aagagttggt tccccggtga
     1081 tgattttgcc tgcgatgccc actctgagtc actcgttttt tcagtgcaaa tttatccgac
     1141 gggatggaat cgcgcgatcc aacggggctg tgaatcagtg gttcaatcgg tcgatatcac
     1201 atgcaaaccc ccacgtgttc tctcccttca gattcgcgac cctgtgatct gcgtgcgaag
     1261 gtttttcttt ctcgccgacg agcaaaccga aggatcgccc tcgcagcaac ggtgtcaaaa
     1321 cgtttcgggg tcaaatgcgg cacagagccg tcagcaggaa tctcaaaatg atccacagac
     1381 acccggccgt tcacgcttga ggtgctgtgc cgaagaacta ttgcggctag ttaccgcggg
     1441 gcatcagcac cgcatcgaag tgcccgaaga cgtcatcaaa ttggaggacc gcctccgtta
     1501 tttgctacaa ccttcgctgg aggcgattct tgccgagcgg caattggaat ttccctttac
     1561 gccgtttccg taccaacttg aaggagttgc ttttcttttt ccccggcatg cggcgatcct
     1621 ggccgacgaa atgggtttgg ggaagaccat gcaggccatc acggccattc ggttgttact
     1681 tcggtgtggc gaggtacggc gcgtcctcct ggtatgtccc aagccgttgg tcagcaattg
     1741 gcggcgagaa tttgagctct gggcccccga gattcccgta atggtcattg aaggtgatcc
     1801 gcaaaggcga aagtggcagt gggaattggc ggacgtcccg gtgaggatcg ccaactacga
     1861 gttgcttccc agggaccaag agtttttcac agcgagtgat gaaacgggcc agacaaaggt
     1921 ggccttcgac ctcgtcgtcc tggacgaatc gcaacggatc aagaaccgtc atagcgcgac
     1981 caatcaggcg gtgtgctcga tcgccagaaa gcggagttgg gccttgacgg gaacgccggt
     2041 cgaaaatagc acggatgacc tggtggggat tttcgagttt ctctctcccg gctttctttc
     2101 ccccgacatg aagccccaac ggctaggacg cactattgcc gactatgtgc ttcgtcgtac
     2161 aaaggatcgt gttctttcgg acttgccgcc caagattctg cgcgatgccg aggtcgagtt
     2221 gactcccgag cagagggaaa cctacgacct tgcggaaacg gccggtgtcc tccgtcttac
     2281 cgaactaggc gatgctgtca cgatccaaca cgtgtttgag ttggtgattc gtctcaaaca
     2341 aatctgtaat ttcgaccccg ccactggggc aagcgccaag atggagcgtc ttgaggccga
     2401 tctcgaagag attgcaggca gcggtcggaa ggccatcatt ttcagtcaat gggtggccac
     2461 attggaagag ataggccgtc gattggaacg atacaaaccc ctctattttc atgggcaaac
     2521 gccggttcgg cagcgtgatg aggtgattcg tcgcttccgc gaagacccac aacaccgcat
     2581 cctgctgatg agttacgggg ccgggagtgt gggtctcaac ttgcagtttg taaattacgt
     2641 ctttctcttc gaccgttggt ggaatcccgc ggtggaggat caagccatca atcgggccca
     2701 ccggatcggg gccgccggcc cggtgatcgt cacccgattt ctcaccgtgg ataccatcga
     2761 acagcggatc aacatggtct tggaacaaaa gcgacagctt ttcgagacga ttttctctgg
     2821 gaccgtggag cctaagaaac tcgcactcac gcaggaagaa atctttggcc tgtttgacct
     2881 caaaacgatg gccacccgag ccaaggccgc ttgaaggaac ctactcgttt gtggggaatg
     2941 gcattccaaa ggacggcggg cggtgggctc tcgtcgggtc tgcatgccat tccacggtgg
     3001 caggcacttt ttcaaaaaat tttttgcgtg cgctgggcga gcgatcggca ataccgtttt
     3061 ttgagagcac ataacgtgct caaaaaacga gagtgtttgc tcgcccgtgt gacggccggc
     3121 aaagtcggtt ttcggtttat ggtagatcag gggaggcgaa tcggacccgg gccggccgtc
     3181 gcgtgggtgt ttaaaaaatc accttgcgcg aagcacaacc cccgcttccc tttgaaagag
     3241 tggatatgtc gcctcttttc ggtgcggcgt atccgctctt tcgctttctt ggggccttct
     3301 gatgcgttga cggggttgat cgccagtgtc gaggatacac tgcgccagat cgaaccgagg
     3361 ccagcttccg atagtgccct cggccgggtt taaccgatct cctcgagggc ttcacggaac
     3421 cgctcgagcg gtgccaagcg tgccccctgt ttgatccacg ggcgaaaagt gtcgagcctt
     3481 tccccatcgg ctaacaccca atcggaggca cgaacgacac ccttgggcgg ggtctggcct
     3541 ttgattatga caagaacccg acattcggca tcagtgcgga agcttctcgc agaccagtcc
     3601 caaatcagga tctcgacctc gggacggtct gaagtccgtc cgccaaatgg ggatgaaggg
     3661 catccgtctg gcgggcatag ctccgcccca caatagtccg agattgcggc tggattgccc
     3721 gtccagacca catggaggtc cttccacgac cgctttttta ggccattccg gcaacatgat
     3781 agtctccccg cccggtgaag ataggcaaag ttgagaccat tgctaacttt tgacggggcc
     3841 gactccactc gggggtgcca aaggtggtaa gtccttgtga gtcggcggat ggaaaacaca
     3901 cggatgccgg cctggtggag ccgtaagccg agatcatcat cttcacaacc ccacccccgg
     3961 tattgttcat cgaaaccgtt gatccgcagg aaatcgtgcc gccacacggc caaattgccc
     4021 gacaggaccc ggggctttcg cggatggcga atcgcgtggt agaagacggc cttccaatgg
     4081 gcaatcgcca gccgtcggta ttctgcccgg agcggccagc ggagataatc tgcgtttttc
     4141 acggcgtctt cggtcatccg ttgggtcatc atttggtcca gacgaataca gtcgcccgtc
     4201 catgcccgat gagggcgccg catccgcata tgagcggcta gatgatctgg ggggatgaca
     4261 cagtcgccat cgagaaaaag gagataccgc cccgtgctag cgatgaagcc ttcattccgg
     4321 gctcgggccg gccaaaaccc ctcgtgggga tgtgtgacaa aacggacggg aaaatcaacc
     4381 tggcgtgcaa attcctcgac aacttgaggt gtttcgtccc gggatccgtc gtccgcaacg
     4441 accacttcca gacggcaatg acagtcccgc tgaatggcca gcgacagtaa gacacgacgc
     4501 aaatgccaag gccgctcaaa cgtcgtgatg atcacggata ggtcggcttc cattccacgt
     4561 ccgctttcct gtcaaggttt tcgtgacggc ggcaaaaagg tgattccttt gaaagcgaca
     4621 cctcacgacg gtccgagatt actcaaaatg cacttagaat tgatcccaaa aagtctccct
     4681 ctccctctgg gagagggttg gggtgagggc aagcgaccgt cggagtcgat cgagaacacc
//
LOCUS       DAOPTR010000150         4738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_14603_length_4738_cov_4.146914, whole genome shotgun sequence.
ACCESSION   DAOPTR010000150 DAOPTR010000000
VERSION     DAOPTR010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14603_length_4738_cov_4.146914"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PKI05_04160"
     CDS             complement(<1..489)
                     /locus_tag="PKI05_04160"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOA51424.1"
                     /translation="MGTRIFGWMTQYWRALFHPQVDEEAVQQSLRAIKERLPTPVVWL
                     LGKAQSGKTSIIHALTGDPRAKIGNGFEPCTKSSLRYAFPDKEFPLVEFLDTRGLDDA
                     SYDPSEDVKWHEQQSHLVMVVMRVMDHAQGSLLAIVEDLRRRKPDWPIIVVQTCLHEG
                     YTW"
     gene            complement(489..3068)
                     /locus_tag="PKI05_04165"
     CDS             complement(489..3068)
                     /locus_tag="PKI05_04165"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOA51425.1"
                     /translation="MRLPLLPERSVESALILMWLVVVGLCPWNVGCRRPQISPEAKSW
                     PAVAEDPREKEGEPGTWPMFRGPNAQGIGVEAHPPVAFGEDHNLRWRVAVPGSGNSSP
                     VVWHDRIFLTTAEGGRSDANGVVLAYRRSDGALLWRYEVGPVKGRTHTKNGYASPSVA
                     CDGERVVAFFGSAGLVCLDLDGNLLWRKDLGPQEHEYGVGSSPIIVGDAVIQLCDREQ
                     DSFLIALDKKTGDELWRTPRPTVGCWTTPVVFSQGNNKGQPVLVVNGGKLESVGRVAA
                     YDLATGRELWSRDEIETLVTPVAVFAENVLISMSGRNGPILALSFPQDGAAQAPAMLW
                     KLPRGGPYIPSGLLYRNRLYILSDARYVACYNPGNGALIWRGSLNGTFTASLVAAEGR
                     IYAVNERGIVFVLNAGDEFRLIAENRIGGRCLATPALVEDDFVLRTDKFLYCFSEKAN
                     RETKPEDSPAVKQTQSGDAPPAQPQEPEQLKTEPSNSNAMVPAEIPADGWPVFRRTPE
                     ATGFVISKIGANLKTRWIFSTEKDQFTATPIVAENTVFLGGAEGIFYAVDLKEGTVRW
                     KAEGYPGFAAPAGYYKGKVYVGDLDGRFHCFQAKDGQHLWQLETGAEIDGGPAFVTLP
                     SGKTVVLVGSQDSNLYCVDADTGEIVWKAASGDQIRCTPPVIKGRTFVAGCDGKLHVL
                     ALEDGRELAAVDLGGPTGSTPALVDSWAYVGTENGVFYGIDWEKAQVVWKFEDTKSGD
                     AFRASAAVNERYVVVGSRDRRVHCFDRITGEHRWQFATRRYVDASPVIVRHKADQNPD
                     EDWVIAASLDGHLYGLRIGNGEKVFDITVGGGMSASPVVAERCLIVATEDGDLACFVS
                     AGE"
     gene            3209..>4738
                     /locus_tag="PKI05_04170"
     CDS             3209..>4738
                     /locus_tag="PKI05_04170"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOA51426.1"
                     /translation="MASFTSMAAWHQPMVPVVLAFGLGIALDRWVWGDTSPPALWIGY
                     MMMGLAVLAISRWMSTRSQNRWALAMLFLSVLWAGAARHHLHWRVFPDNHIGRFASDE
                     PQPICLEAVLSSVLEPSALSAEAMTFPEPKPFLRGVAKAVALRDGTTWRPVSGRVLLY
                     VAGSESPYQPGDRLRIVGRFQRLAQPQNPGQFNLREYRRADRILAQVTVDYPEGITHI
                     DSATSWQPLAILGRWRAAARLRLHLLLPESDHALAQALLLGFRSEIPDDFEEALLKTG
                     TIHLLAISGLHVGILAGGFSLGLQLVRFPRRLRPVALLLVIVVYVALAGGQPSTVRAS
                     ITLGLFLVGHLLYRSTFSINTLAVAAMTLLILNPSDLFRVGPQLSFLAASVLLVVQST
                     KLQPTDPLLVSGFGPPSAWARFIRSLRQRVVSLLLLSVTVWLITLPLVIQQFHILNPL
                     SVILTPILVFPITVALLTGFLLILLGPVLSPLTACLAWLCHVSLSITESVIVSTCQNL
                     PL"
BASE COUNT          951 a         1540 c         1221 g         1026 t
ORIGIN      
        1 ccaggtgtaa ccttcgtgca agcacgtctg gacgacgatg atcggccagt cgggttttcg
       61 gcggcgaagg tcttccacta tggcgagtaa cgaaccctgg gcatgatcca tcacccgcat
      121 gaccaccatc acaagatgcg actgctgctc gtgccatttc acgtcctcgc tcggatcgta
      181 agacgcatca tccagacccc gagtatcgag gaattcaacc aggggaaact ccttgtcggg
      241 aaaggcgtaa cggagagaac ttttggtgca cggttcgaag ccatttccaa tcttcgcccg
      301 cggatcgccg gtcaaggcat ggatgatcga cgtctttcct gactgggcct tacccaaaag
      361 ccaaacgacg ggcgtcggca accgctcttt gatcgcccgg agactttgtt ggaccgcctc
      421 ctcatccacc tggggatgaa ataaggcccg ccaatattgc gtcatccagc caaagattcg
      481 ggtccccatt attcgcccgc cgacacaaaa caggccaggt cgccatcttc tgtggcaaca
      541 atgagacaac gctcggccac aaccggcgag gccgacatgc ctccgcccac ggtgatgtcg
      601 aacacctttt ccccattccc gatccgcaat ccgtagaggt gcccatccag tgaggccgca
      661 atcacccaat cctcgtccgg attctggtca gctttgtgcc tgacaatcac aggcgaggca
      721 tcgacatacc ggcgggtggc gaattgccag cggtgctcgc ccgttattcg gtcaaagcag
      781 tgaacacggc ggtcccgact gcccaccaca acgtaacgtt cgttaacagc cgccgacgcc
      841 cgaaaggcat cgcccgattt ggtatcctca aatttccaca ccacctgggc cttttcccaa
      901 tcaatcccgt aaaagacgcc gttctccgtt cctacgtagg cccaggaatc cacgagggcg
      961 ggtgtggaac cggtcgggcc tcctagatcg acggcggcca gctcgcggcc atcttccaag
     1021 gccaggacgt gcaatttacc atcacacccc gcgacgaacg tccgtccctt gatcaccggc
     1081 ggcgtacagc ggatctgatc cccacttgcc gccttccaga cgatttcccc agtatcggca
     1141 tccacgcaat aaagattcga atcttgcgag ccgacgagaa ccaccgtctt tcccgatggc
     1201 agcgtcacga aggccggtcc gccgtcaatc tccgccccgg tctcaagttg ccaaaggtgc
     1261 tgaccatcct tggcttggaa gcaatggaac cgcccatcga gatcgcccac atagaccttc
     1321 cccttgtagt atcctgccgg agcggcaaag cctggatagc cctcggcctt ccaccgcaca
     1381 gtcccctcct ttaagtccac ggcgtaaaag attccttcgg caccgcccaa gaaaaccgtg
     1441 ttctccgcga caattggcgt ggccgtgaac tgatcttttt ctgtagaaaa aatccatcgc
     1501 gttttcaaat tggcaccgat ctttgagatg acaaacccgg tcgcctccgg tgtgcgacga
     1561 aagaccggcc agccgtcggc aggtatttcg gcaggcacca tcgcgttgct gttactgggt
     1621 tcggttttga gttgttcggg ctcctgcggc tgcgcgggtg gagcatcccc ggattgggtt
     1681 tgtttaacgg ccggagaatc ctccggcttc gtttccctat ttgctttttc gctaaaacaa
     1741 tagagaaatt tgtctgttcg caggacaaag tcatcctcga ccagggccgg tgtcgccagg
     1801 cagcgtccac caattcgatt ctcggctatc aatcggaact catcaccggc gttgaggacg
     1861 aacacaatcc cccgctcatt cacggcgtag atcctcccct cagcggccac taacgaggcc
     1921 gtgaatgtcc cgttaagact tccccgccaa ataagcgccc cattgccggg gttgtaacag
     1981 gccacatatc gggcatcgga taaaatgtaa agacgattgc ggtagagaag ccccgacggg
     2041 atatacggcc caccgcgagg cagtttccaa agcattgccg gagcctgggc ggccccgtcc
     2101 tgcgggaagg acaaagcgag gatgggaccg ttacgaccgc tcatgctaat gagaacattc
     2161 tcggcaaaga cggctaccgg cgtgacgagc gtctcgatct catcgcggga ccacaattcc
     2221 cgacccgtcg ccagatcata cgccgccaca cggcccaccg actccagctt gccgccatta
     2281 accaccagta cgggttgccc tttgttgttg ccctgggaaa acacgaccgg cgtcgtccag
     2341 caaccaaccg tcggccgcgg agttcgccat aactcgtccc cagttttctt atcgagggca
     2401 atcagaaacg agtcctgttc ccggtcgcac agttggatca ccgcatcgcc cacaattatc
     2461 gggctggaac cgacgccgta ttcatgctcc tgcggtccta ggtcttttcg ccaaaggaga
     2521 ttgccatcga gatcaagaca gaccaatccc gccgagccga aaaaggccac cacgcgctca
     2581 ccatcgcatg ccacacttgg cgaggcatag ccgtttttgg tatgcgtccg acctttgaca
     2641 ggccccacct catatcgcca caacagggcc ccatccgacc gacggtaagc gaggacaacg
     2701 ccgttagcat cgcttcggcc accctccgcc gttgtcaaaa agatgcgatc atgccaaacg
     2761 acaggtgagc tatttccgct gccgggaacg gccactctcc agcgaagatt gtggtcctca
     2821 ccaaatgcca ccggtggatg cgcttcgaca ccaattccct gggcattcgg accgcgaaac
     2881 ataggccatg tgcccggttc cccctccttt tcgcgagggt cttctgccac agcaggccac
     2941 gacttggctt ccggtgagat ttgggggcgc cgacagccga cattccacgg gcacaacccc
     3001 acgaccacca accacatcaa aatcaaggca gattcgacgc ttctttcggg caacaacggc
     3061 aatctcatct tgaggtcctg acgtcaaaat aggcttattc ccaaaccggc cggcgttatc
     3121 tcataatgtc atcattctct ttgcttttgg gattcacaat aggtgtcaac tctcatagag
     3181 atcactgcca cacatgtcaa ggaatgcggt ggcatcgttc acgagcatgg cggcatggca
     3241 tcagcccatg gtcccggtcg tcctggcctt tggcttggga attgccctgg accgttgggt
     3301 ttggggggat acctctccgc ccgccttgtg gatcggctac atgatgatgg ggctggcggt
     3361 gctggccatc tctcgctgga tgagcactcg tagccaaaac cgttgggcct tggcgatgct
     3421 ctttttatcc gtgctctggg caggggcggc tcgacatcat ctccattggc gggtcttccc
     3481 cgacaatcac attggccgct ttgccagcga tgaaccccaa cccatttgcc tggaggccgt
     3541 cctctccagt gtccttgagc catcggccct gtcggcggag gcgatgacct ttccggagcc
     3601 aaaacccttt ctccgcggcg tggcaaaggc cgtggcgctc cgcgatggga cgacctggcg
     3661 tccagtttcc gggcgagtcc tgctttatgt ggcggggtcc gaatcgccgt accaaccggg
     3721 cgaccgcctg cggatcgtgg gaagattcca acggctggcc cagccacaga accccggcca
     3781 attcaatctc cgcgaatacc gccgtgccga ccgcatcctt gcccaggtta ccgtggatta
     3841 tcccgagggt atcacccaca ttgactccgc gacctcctgg cagccgctgg ccattttggg
     3901 acggtggcgg gcagccgccc gattaagact ccacctgctg ctgcccgaat ccgatcacgc
     3961 cctggcccaa gccctcctcc tcggcttccg gagcgaaatt cccgacgact ttgaagaggc
     4021 cctcctgaaa acaggaacga tccatctctt ggccatctcg ggtttgcacg tcggaatttt
     4081 ggccggtggt tttagcctgg gactgcaact cgttcgattt ccgcgacggc tccgcccggt
     4141 ggccctcctt ctggtcatcg tggtttatgt ggccctggcc gggggacaac cctccaccgt
     4201 tcgggcctcc atcacccttg ggctttttct ggtgggacac ctgttgtatc ggtcaacgtt
     4261 ttccataaac accctagccg tggccgccat gacgctcctg atcctcaatc cgagcgacct
     4321 cttccgtgtg ggtcctcaac tttcctttct ggcggcctcc gtcctattgg ttgttcagtc
     4381 cacaaagtta cagcccactg acccactttt ggtatcgggc ttcggaccac cgtcggcatg
     4441 ggctcgcttc attcgatctt tgcgccagcg cgttgtgagc cttctccttc taagtgtcac
     4501 cgtctggttg ataacactac ccctcgtcat ccaacagttt cacattctca acccactgag
     4561 tgtgatcctc acaccgatcc tcgttttccc aatcacggtg gcgcttctga cgggctttct
     4621 gctcattttg ctcggaccgg tccttagccc actgacggct tgtctcgctt ggttatgtca
     4681 tgtctcgctg agcatcaccg aaagcgtgat cgtgtcaact tgccaaaacc tgccgctt
//
LOCUS       DAOPTR010000151         4732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_14629_length_4732_cov_3.855463, whole genome shotgun sequence.
ACCESSION   DAOPTR010000151 DAOPTR010000000
VERSION     DAOPTR010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14629_length_4732_cov_3.855463"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..426
                     /locus_tag="PKI05_04175"
     CDS             46..426
                     /locus_tag="PKI05_04175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HOA51427.1"
                     /translation="MTKQNQIYYCEQCGNMVEVLEAGQGQLVCCEVPMKRIAENVGDA
                     AQEKHVPAVTREGKSLHIKVGSVIHPMTPEHLIEWVEMFQEDRCIRVFLKPGQAPELR
                     VPAAQAPATVRAYCNLHGLWKVDV"
     gene            complement(699..2429)
                     /locus_tag="PKI05_04180"
     CDS             complement(699..2429)
                     /locus_tag="PKI05_04180"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOA51428.1"
                     /translation="MDRFEDKPREYQANSFASSQLQESAHRHTWMPPEDFSSSLQGIS
                     PATFRVLIVDDDALILRLLERWLTKAGYQVSQAKDAETALQMVREEPPHILITDWEMP
                     GMKGPDLCREMRLLRLPHYVYVLIVTSHNESANLITALSQGADDFLAKPIREAELMAR
                     LGAAIRILSAQRQLQEMAQRDALTGLLTQRSFHDFLEKEFEVARRTKRPLSCAMVDLD
                     FFKRINDIHGHQAGDYVLRTTAQLLLASCRASDIVCRYGGEEFCVLLPDATEENAVKW
                     AERFRAELGATPFTIGKTTLSLTCSIGISQVYDDTLTPEQLVDQADQALLCAKRAGRD
                     RLVRYHEISLSEEVNLSDKHRQGDLFEGILARHVMSPIVAPLRGDETVDQAAEFFLRS
                     RISSAPVVDETGRLIGMMSEKDLLSAMTSVHGWSRPISDLMRPHVITYPLDAPVRIIY
                     EFLCRVSIRRVIITDNGVPIGAISRGSLLRWFRNLVLSKAYAKEDAGVPYNSGDTRLA
                     ALREALAAISLEAQQLRQTLDKNAPDVVAHVVGAASRIQELATDMLAFANIARDEAIS
                     PALGAAALWD"
     gene            complement(2594..>4732)
                     /locus_tag="PKI05_04185"
     CDS             complement(2594..>4732)
                     /locus_tag="PKI05_04185"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51429.1"
                     /translation="GLSLGVKDFLHRVQTSESHLGDSVCGLVADLLQTELQVALLVDV
                     ALGDAAEWIVLRNSMPWLDQLRRGSAALNGRVCLLCLDQFRKKADGQSMPTDISSLGQ
                     EPGVLGLAEDFVQVEEEFLPVRHFLLGKTWFVESLDTALALASRYADLRFVTLAGELI
                     TSEGLIVAGQQSSGLGLVSRRSELRNLTRSIAQWDQQLQQYQEAIATSEHQLKTVEQR
                     IANLEEQRRSLSQELKDCEQRQQELQRMLQEYLGRCGALEGEIAAAAEGVERIVQQQQ
                     QALLEKTQWEEKAAELSIAITQLGEEIAQLEANVQDRHGVLTALKIELAKSEERLAAL
                     QNQLRRQEEAEAERLKEVTEIKNRLAETEKRLMASQRELLSLEARLAELYGRKDELSR
                     VWQETAEARRFLQKERQKHQQQLQDVRRRLRQIEERQHKLELDLNSVRHEKSTLIQRV
                     EEDYGISLNDLDHPCSPEESQDRTEIQREIDDLKRKIHNLGNVNLEALEELQQLEERY
                     NGLSSQCRDLTSAKNSLERIIERINADSRRLFAATLQTVSEHFGTLFRELFGGGQASI
                     ELEPGRDILESGIEIVARPPGKEPRNISLLSGGEKSLTCVALLLAIFRSRPSPFCVLD
                     EVDAALDEANIDRFTKVLRDFLSITQFVIVTHSKRTAICADTLYGVTMQESGVSKRVS
                     VRLEDLSDQGEILSAGSVQSFSTSPEEKAA"
BASE COUNT         1078 a         1293 c         1247 g         1114 t
ORIGIN      
        1 ctgacagtcc gttcgcgatg cgtgtcactg agaaggaggt gaggtgtgac taagcagaat
       61 cagatctact actgtgagca gtgcggcaac atggtggagg tcctggaggc cggacagggg
      121 cagcttgttt gttgcgaagt gcccatgaaa cgcatagcgg aaaacgtggg cgacgccgct
      181 caggaaaaac atgtgcctgc ggtgacacgg gagggcaaat cacttcatat caaagtggga
      241 agcgttatcc atccaatgac gcctgagcat ctcatcgaat gggtcgagat gtttcaagag
      301 gatcgctgca tccgcgtgtt cttgaagccg gggcaagcac cggaattgcg ggtacccgct
      361 gcccaggcac ccgctaccgt ccgcgcgtac tgtaatttac acggcttatg gaaggttgat
      421 gtctgagcgg ggaaatatgt catccccaga caacgactgt gccggcaaat acggcaaggg
      481 caagaaacgc acaaacgagg agagtttgaa tcgctatcac cgtcaggtcc tctagcacct
      541 ttccaatcgg gagaaaaatt ggccccgagt ctttcatcgg gatttgttta gcgaaccacg
      601 aggagaaagg tagttcgcca gataaagtct gtgatccgca aatctttccc gatcagaatt
      661 ttttgggcca tgagaagaca aatggttccg cagtttcctt agtcccagag cgcagcggcg
      721 cccaaggcgg gactgatcgc ttcatctcga gcgatattgg caaaggcaag catatctgtg
      781 gccagttctt ggatgcgact ggcggccccc acaacgtgtg ccacgacgtc aggggcattc
      841 ttgtcgaggg tttgccgcaa ttgctgtgcc tccagggaga tcgcggcgag ggcctctcgg
      901 agggctgcta gccttgtgtc tccagaattg tagggaactc ctgcatcttc ctttgcatag
      961 gccttggaaa gaacgagatt cctgaaccag cgcaggagcg aaccccgact gatggcaccg
     1021 atggggacac cattgtccgt aatgatcacc cgtcgtatcg aaactcggca tagaaactca
     1081 taaataatcc gtacgggagc gtccagtgga taggtgatga cgtggggtcg catgagatcg
     1141 gaaatcggac gactccaccc atgtacagat gtcatggccg acagaaggtc cttttctgac
     1201 atcataccaa tcagtcggcc tgtttcatcc acgacaggag ccgaactaat gcgcgatctg
     1261 agaaagaact cagcagcctg atccaccgtt tcgtcgccac gtaatggggc aacaatcggc
     1321 gacatcacat ggcgtgccag tatcccttcg aacaaatcac cttgccggtg tttatcgctc
     1381 aggtttacct cttcacttaa gctgatctca tgataacgaa cgagccgatc gcgaccggca
     1441 cgtttggcac aaagaagggc ctgatctgcc tgatcgacga gctgttctgg ggttagcgtg
     1501 tcatcataaa cttgagagat tccgatactg catgtcagag aaagcgttgt tttcccgatc
     1561 gtaaatggtg tggcgcctag ctccgcgcga aaacgttcgg cccacttgac ggcgttttct
     1621 tcagttgcgt ctggcaaaag cacgcagaac tcttcgcccc cataacggca cacgatgtcg
     1681 ctggcccggc aacttgccag aagcaactgg gccgttgtcc ttaagacgta atcccctgcc
     1741 tggtgaccgt ggatgtcgtt aatacgttta aaaaagtcca aatcaaccat tgcgcagcta
     1801 aggggccgct ttgtcctccg tgcaacttca aactcttttt cgagaaaatc atgaaagctc
     1861 cgctgcgtca gcaaacctgt cagggcatct ctttgggcca tctcttggag ctgtcgctgg
     1921 gcggagagga tacggatggc cgcccccagt cgggccatta actcggcttc ccgaataggt
     1981 ttggcaagga agtcatcggc cccttggcta agcgccgtga ttagattggc cgactcgtta
     2041 tgggaggtca cgattaggac ataaacataa tggggaagcc tcaggagcct catttcacgg
     2101 cacagatcag gccccttcat ccccggcatt tcccagtcag tgatgagaat gtgaggcggt
     2161 tcctcgcgaa ccatttgcaa ggccgtttcc gcatccttgg cctggctgac ttgataaccg
     2221 gccttcgtca accaacgttc gagtaaccgc aagattaggg catcgtcatc gacgattaag
     2281 acgcgaaatg tggcaggcga aattccttgc aacgacgaag aaaaatcctc cggcggcatc
     2341 caggtatgcc tgtgggccga ttcctggagc tgtgacgaag caaaggaatt cgcctggtat
     2401 tctcgtggtt tgtcttcaaa cctatccatg ttgcgcactg tcattgctcc ttaggttcgc
     2461 gacgtcaaaa aaacatagac taccgtttca aggaaggcgt gataacaccg gcagtttttt
     2521 ccggttaaat agatactttc aatttctcag gtgtggtggg tggcggattt tgcggaactt
     2581 tttggaatag cggttatgcc gctttttctt cgggcgaagt ggagaaggac tgtaccgaac
     2641 ctgctgagag aatttccccc tgatcgctca aatcctccag ccgtacggag acccgttttg
     2701 atacacccga ctcttgcatc gtgaccccgt acagcgtatc tgcgcagatt gccgtgcgtt
     2761 tggaatgcgt aacgataaca aattgcgtga tgctgagaaa atcccgcaga acctttgtaa
     2821 aacgatcaat gttagcctca tcaagggcgg catcgacttc gtccaaaacg cagaagggac
     2881 tcggacgact tcgaaagatc gcaagaagca gggctacgca tgtgagactc ttttctccgc
     2941 cactaagcaa ggagatgttt cgtggttcct tccctggcgg gcgggctacg atttcgatgc
     3001 cactttccag gatgtcccgc cctggctcaa gctcgatgct tgcctgacca ccgccgaaaa
     3061 gttctcggaa gagggtgcca aagtgttcac tgaccgtctg caatgtcgcg gcaaaaagcc
     3121 gccgactatc ggcattgatc cgctcgataa ttcgttccaa ggagtttttt gccgaggtca
     3181 agtcccgaca ctgggacgac aagccgttgt agcgttcttc cagttgttga agttcctcga
     3241 gggcttcgag gttgacgttc cccaagttgt ggattttacg tttcagatcg tcgatttccc
     3301 gctggatttc agtgcggtcc tgcgactcct caggggaaca cggatggtcg agatcgttca
     3361 gagaaatccc ataatcttct tcgactcgct ggatgagtgt tgacttttcg tgccgcacag
     3421 agttgagatc aagttccagt ttgtgctgcc tctcctcgat ttgacggagc cgtcgccgca
     3481 catcctgcaa ttgttgctga tgtttttgac gttccttttg gagaaaacga cgggcctccg
     3541 cggtctcttg ccaaacccgc gaaagttcat ccttccgccc gtacaattcg gccaggcggg
     3601 cctccagact cagtagttcc cgttgtgagg ccatcaaccg cttttcggtt tcagccaaac
     3661 ggtttttgat ttccgtgacc tccttaagcc gttcggcctc ggcttcctct tgccgtcgca
     3721 gttgattctg gagggccgcg agccgttctt cgctcttggc caattcgatt ttcaacgcgg
     3781 tcaacacacc gtgacgatct tgaacattcg cttccaattg ggcaatttct tctcccaatt
     3841 gagtgatggc gatggacaac tccgctgcct tttcctccca ttgcgtcttc tctaaaagtg
     3901 cctgctgctg ctgttggaca attctttcaa ccccctcggc agccgcggca atttcgccct
     3961 ccagtgctcc acagcgtccc aaatactcct gcaacatccg ttgcagctct tgttgccgtt
     4021 gctcacagtc tttcagttcc tgactgaggg aacgacgctg ttcctcaaga ttggcaattc
     4081 gttgctccac agtcttgagc tgatgttcac ttgtggcaat ggcttcctgg tactgttgca
     4141 actgttggtc ccattgggca atcgaacgcg tcagattacg aagttcgctc cggcgcgaaa
     4201 ccagcccaag tccagaactc tgttgcccag ctacgataag cccctcggaa gtgattaact
     4261 ccccggcaag ggtcacaaat ctcaagtcgg cataccggct ggccaaagct agggccgtgt
     4321 ccaaggactc gacaaaccaa gtcttgccaa gcagaaaatg gcgaaccggc aggaattctt
     4381 cttccacctg aacgaagtct tccgccagcc ccaggactcc cggttcctgc cccagggaag
     4441 aaatgtcagt gggcatgctc tggccatcgg ccttttttcg aaattgatca agacacaaca
     4501 gacaaacccg gccgttgagt gccgcagaac cgcggcggag ttggtccagc caaggcatgc
     4561 tgttgcgcaa gacgatccat tccgcggcat cacccagggc cacatccacc aaaagggcta
     4621 cctgaagttc tgtctgcaac agatcggcca ccaagccaca aacggaatcg ccgagatggc
     4681 tctccgatgt ctggacgcga tgaagaaaat ccttgacacc gaggcttaag cc
//
LOCUS       DAOPTR010000152         4705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_14742_length_4705_cov_3.297849, whole genome shotgun sequence.
ACCESSION   DAOPTR010000152 DAOPTR010000000
VERSION     DAOPTR010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14742_length_4705_cov_3.297849"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PKI05_04190"
     CDS             complement(<1..646)
                     /locus_tag="PKI05_04190"
                     /inference="COORDINATES: protein motif:HMM:NF037435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HOA51430.1"
                     /translation="MPDRRRHRGPHPEDRQLFDAAMYPRLQAAFSDLCWLLDRGYAVT
                     SSLKLVGDRYELSARQRLALERCACTAAAVAARQSRLCPPQELAGRAICLDGYNVLTT
                     IEAALAGGVLLGGRDGTLRDMASMHGSYRKVAETQQAIELVGNTLASLGVSEAVWYLD
                     KPVSNSGRLAQLLRTLAAEHQWNWEVNIVPSPDHELQARGIPVATADHVILDHCQ"
     gene            complement(757..1521)
                     /locus_tag="PKI05_04195"
     CDS             complement(757..1521)
                     /locus_tag="PKI05_04195"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HOA51431.1"
                     /translation="MGTLSRMIGFGSLIGLPALILLAMFCEGCGPPGTTNATPQPFNI
                     VGEAPSCCQEANQETSQQVSPAANPQGTQEVAQTPSVEGSLKPKEGGRIMKMKLTSPA
                     FEPGQPIPKQYTGEGADVSPPLEWSNVPEGTQEFALICDDPDAPTAEPWVHWVIYKIP
                     ADVTKLPEGIAKTPKLSVPPGALQGKNSWPSGQTIGYRGPMPPPGHGRHRYYFRLYAL
                     DKKLDLEPGLTKKQLLEAMKGHILGEGELMGTYERR"
     gene            complement(1562..3025)
                     /locus_tag="PKI05_04200"
     CDS             complement(1562..3025)
                     /locus_tag="PKI05_04200"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOA51432.1"
                     /translation="MSKDAYSGPLERIDETTWRIPRDYKPGMQVDGIIYADDRLIEQI
                     RSDQAPEQVANVAFLPGIQSASLAMPDIHWGYGFCIGGVAATDPQAGGVISPGGVGYD
                     INCGVRLIRTNLHVDDIKPRLGGLMNVLFQKIPAGVGKGGPYLFAGKQMDQILEEGCH
                     YLKKIGLATEQDIQHTEETGCIPGADASCVSQEAKKRGSDQCGTLGAGNHFLEVQVVE
                     EVFDPVAAQVMGLKEGGICVMIHSGSRGLGYQVCDDALKEMRRCPEKYHTELPDRQLA
                     CAPIESPEGQRYLAAMRAAANYAWANRQLLMWQTREAFSEFFGKPWEELQINLVYDVA
                     HNIAKMEKHIIDGKEKLVCVHRKGATRGFPAGHPDIPDRYRSIGQPVIIPGDMGRESY
                     VLVGLPAAMEKTFGTTCHGAGRVMSRNEAVRHARGRQIDKELAEKGIIARAHSRAGLA
                     EEHSDAYKDVSIVVDVVERAGLAKKVARLRPLGVVKG"
     gene            complement(3314..3703)
                     /gene="queF"
                     /locus_tag="PKI05_04205"
     CDS             complement(3314..3703)
                     /gene="queF"
                     /locus_tag="PKI05_04205"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HOA51433.1"
                     /translation="MPDRLRRTAVSQGFRHILETFENQYPDRDYTIEIICPEFTSVCP
                     KTRQPDFGTITILYSPDKLCVELKSLKFYLQAYRDVGIFYEHVTNRILDDLVAVLQPR
                     WMKVIADFRPRGGITTRVTAQYRAEHP"
     gene            3804..4529
                     /gene="queC"
                     /locus_tag="PKI05_04210"
     CDS             3804..4529
                     /gene="queC"
                     /locus_tag="PKI05_04210"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOA51434.1"
                     /translation="MPQNTDSVRAGPAQRKAIILLSGGMDSATTGAIAREEGYLLYAL
                     TVDYGQRHYRELEAAKRVAEFLGVAEHIILKVDLTAFGKSALTDTIDVPKDRPIAEMP
                     KEIPITYVPARNTVLLSLALAYAEARGAWDIFVGANAVDYSGYPDCRPEFISAFETMA
                     NLATKAGVEGTGRFRIHAPLLRLSKAEIIHRGLALGMNYGLTHTCYDPTPDGRACGRC
                     DACQLRKKGFLEAGVPDPIPYAE"
BASE COUNT          991 a         1349 c         1297 g         1068 t
ORIGIN      
        1 tttgacaatg gtcgagaatc acatgatcgg ccgtggccac ggggatacct ctcgcttgta
       61 actcgtgatc gggactgggc acgatgttga cctcccaatt ccactggtgc tcggccgcca
      121 aagtgcgcaa gagctgtgcc agccgaccgc tgttcgacac aggcttgtcc agataccaca
      181 cggcttccga cacaccgagg gatgctaggg tgtttcccac cagttcgatg gcctgttggg
      241 tctcggccac ctttcgatag gagccgtgca tgctggccat gtcgcggagg gtgccatcac
      301 gtcctccgag gagtacccct cctgccaagg ccgcctcaat tgtcgtcagc acgttgtagc
      361 catcaagaca aatggcacga ccagcaagct cctgcggcgg acacaaccgc gactgccttg
      421 cggcaaccgc cgctgccgtg catgcgcacc gctcaagagc caatcgttgc cgggccgaaa
      481 gttcgtacct gtcgcccacc agtttcaacg acgaggtgac cgcgtaaccc cgatccagca
      541 accagcaaag atcggaaaat gctgcctgca atcggggata catggcggcg tcgaatagct
      601 gccgatcttc cggatgggga ccacgatgtc gtcggcgatc cggcacagtt cacctgctgc
      661 tcaatcaagt tcaaaaccca gcccttacgg accatgacga acacgttgga ggcggcatgc
      721 cgccgagcca gtctcggcta tcggaagtag ccggggctag cggcgttcat aagtgcccat
      781 cagctcgcct tctcccagga tatggccttt catcgcctcc agcagttgtt ttttggtaag
      841 gcctggttcc agatcgagct tcttgtccag agcatacaga cggaagtagt accgatggcg
      901 accatgacct ggtggcggca tgggcccgcg atagccgatc gtttggccgc ttggccaaga
      961 gttcttcccc tggagggcgc ctggcggaac tgaaagtttg ggcgttttgg caattccttc
     1021 gggaagcttg gtcacatccg ccgggatttt gtaaatcacc caatggaccc acggttctgc
     1081 cgtcggggcg tctggatcat cgcaaataag cgcgaattcc tgcgtcccct cgggaacatt
     1141 tgaccactct aagggtggcg acacgtcggc tccttcccct gtgtattgct tgggaattgg
     1201 ctgcccgggt tcaaatgccg gacttgtcag tttcatcttc atgatacgac ctccttcttt
     1261 tggcttgagg gacccttcca cagacggtgt ctgagctacc tcttgcgtgc cctggggatt
     1321 ggcggcaggt gatacctgtt ggcttgtttc ttggttagct tcctgacaac aagagggggc
     1381 ttccccaacg atgttaaacg gctgcggcgt ggcgttcgtc gtaccgggcg gtccacatcc
     1441 ttcacaaaac atcgccagga gaatgagagc cggtaggccg atcaagctgc cgaagccaat
     1501 catacgacta agagtcccca tggctgaccc tcccttaaga tcacacaggt ttgaaaaagg
     1561 gttatccttt gacgacaccc aaaggccgca ggcgggcgac tttcttggcc agccccgccc
     1621 gctcaacaac gtcgactacg atactgacat ctttataagc gtcggagtgt tcctcggcga
     1681 gccccgcccg actatgcgct cgcgcaatga tccctttttc ggcaagttcc ttgtcgattt
     1741 gtcgtcctcg agcgtgccgc acagcttcgt tgcgactcat cacccgacct gccccgtgac
     1801 aggtggtgcc aaaggttttt tccatggctg ccggcaatcc caccaggaca taactttctc
     1861 gccccatatc ccctgggatg atcaccggct gaccgatcga tcgataacga tctgggatgt
     1921 cgggatgccc ggcggggaat ccccgcgtag cccctttgcg atggacgcac accagttttt
     1981 ctttgccgtc aataatgtgc ttttccatct tggcgatatt atgcgccacg tcatagacga
     2041 ggttaatttg cagttcctcc caaggcttgc cgaagaactc gctaaaggcc tcccgcgttt
     2101 gccacatcaa gagctgccga ttggcccagg cataattggc cgccgcccgc atggccgcca
     2161 aatatcgttg tccctccggg ctttcgatgg gcgcacaggc taattggcga tctggaagtt
     2221 cggtgtgata tttctcgggg cagcgacgca tttccttgag ggcgtcatcg caaacttgat
     2281 agcccagacc gcgggaaccg gagtggatca tcacgcaaat gccgccttct tttagcccca
     2341 taacctgggc ggctacgggg tcaaagacct cttccaccac ctgcacctcg agaaagtgat
     2401 ttcccgcccc cagtgtcccg cattgatcgc tgccacgctt cttcgcttcc tgactgacgc
     2461 acgaggcatc ggcccctggg atacaccctg tttcttctgt gtgctgaatg tcctgctcgg
     2521 tggccaggcc gatcttcttc agatagtggc aaccctcctc gagaatttgg tccatttgct
     2581 ttccggcaaa aagatagggt ccacctttgc ccacaccggc aggaatcttc tgaaaaagga
     2641 cattcatcaa accgcccaat cgtggtttga tgtcgtccac gtggagattg gtccgaatca
     2701 gtcgaacacc acaattgatg tcatatccaa caccccctgg tgaaatcaca ccaccggcct
     2761 gcggatcggt cgctgcgaca cccccgatgc agaatccata gccccaatgg atgtcaggca
     2821 ttgctaggct tgcggactga atccccggca agaacgccac attggcaacc tgctcggggg
     2881 cctgatcgga gcgaatctgc tcgatcaggc gatcatccgc atagatgata ccgtccactt
     2941 gcattcccgg tttatagtcc cgtgggatgc gccacgtcgt ctcgtcgatg cgttcgagcg
     3001 gtccagagta tgcgtcctta ctcatgattc agcctccttt taaggcctaa acgttctctg
     3061 attcttctga catctgccca aatgcgaggt gtcaaaagag ttgcggttcc ctttccatga
     3121 tgagggcctt atgttggtcg gcatggtagc gtcctgacgg ggaagctgag accctcgcca
     3181 atttaacatt ctgaaccggg ccaacattca tcccgcgtcc acttgtcatt ctatgagact
     3241 gggacgactg tcaaaaatca tttaagatgt ctggctaaga tatctggcct cagataggca
     3301 caggtttacg agtttatggg tgttcagcac ggtactgggc cgtgacgcgg gtcgtgatac
     3361 caccccgggg acggaagtcg gcgataactt tcatccagcg tggttgcaga actgccacca
     3421 gatcgtcgag gatgcgattc gtgacatgct cgtaaaaaat acctacatcg cggtacgcct
     3481 gaagatagaa ctttaggctc ttcagctcca cgcagagctt gtccggtgaa tataggatcg
     3541 taatcgtccc gaaatcaggc tgtcgggtct taggacagac cgaggtaaac tcagggcata
     3601 taatctcaat ggtgtaatct cggtcaggat actgattttc gaacgtctcg agaatgtgac
     3661 gaaaaccttg actcacagca gtcctcctta aacgatcggg catttctgtc catcatatag
     3721 gagcggggga ctcctgccca cacgtggcga ggcatgtcag gattgcccgc acaaaaaggg
     3781 aatcagccac aggaatcgcg tgaatgcctc agaacaccga cagcgtcaga gctggaccag
     3841 cacagcgtaa agctattatt ctcctatcgg gtggaatgga ctccgcaaca acgggggcca
     3901 tcgcccgaga ggagggctac ctgctgtatg cgctgacggt ggattacggg cagcggcact
     3961 accgggagtt ggaggcagcc aagcgagtgg ccgagtttct gggcgtggcc gagcatatta
     4021 ttctcaaggt ggatctcacg gcttttggaa agagtgcgtt gacggacacg attgatgtcc
     4081 ctaaggatcg tccgatcgcc gaaatgccga aggagatccc gataacgtat gtcccagccc
     4141 gcaataccgt tttgttgtcg ctagccctgg cgtacgcgga ggcccggggg gcgtgggata
     4201 tctttgtcgg ggccaatgcc gtggactaca gcggttatcc cgattgccgg ccagagttta
     4261 tctccgcctt tgaaacgatg gccaacttgg cgacaaaggc aggggtggaa gggaccgggc
     4321 gatttcgcat ccatgccccg ctgttgcggc taagtaaggc ggagattatc catcgggggt
     4381 tggccttggg aatgaattac gggttaaccc acacctgcta cgatccaacg cccgacggac
     4441 gggcatgcgg tcggtgtgat gcctgtcagc tgcgaaaaaa gggatttctc gaagccggcg
     4501 tcccggatcc catcccttat gccgagtgac gatttttggt tgaaacatgg ctcaaacagt
     4561 gcctggcatt accgatatag gattccaatc tttgggacaa cagtacatcc gcattgccga
     4621 ggttttccaa tctcggcagg gggaagggct tttgacgggt acagagagtg tgtttgtccg
     4681 cacctctggg tgtaaccttc gctgt
//
LOCUS       DAOPTR010000153         4663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_14898_length_4663_cov_4.601128, whole genome shotgun sequence.
ACCESSION   DAOPTR010000153 DAOPTR010000000
VERSION     DAOPTR010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14898_length_4663_cov_4.601128"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3622)
                     /locus_tag="PKI05_04215"
     CDS             complement(<1..3622)
                     /locus_tag="PKI05_04215"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOA51435.1"
                     /translation="MARTVNKKLAVILVGAVVLAIVSGYLLHGFQTRRNARVFLDRAR
                     EAKTQERFDVAVKNYQWYIDLVPNDLDALSEYGSLLADLKAYWPAFTRLEMVLRQDPG
                     RLETRRKLVQVLTAMGRFRDAQEHIEQYLLKEYPDDPELLTELGRCQRGLGQYERATT
                     TFRRVIELKEDYVPAYAQLAGTYRHDLKQPEQGDQWITKMVLTVGDSAEAYLVAASYF
                     YNAQNYEAALKWADEAHKKDPSNLEMLLVAADSARRLGQLDQAEEFAQKAIAASPESP
                     DPYSVYAATQFAKGNADTAINTLEDAASKVKDKGPLYWALASMQIDALRLADAEQTIE
                     KLEAVSYPRAQVEFLRGRLLTAKEQWFLAARKLESVRPDVTDSPDLLKQVDFLLGLCY
                     AQMGNPDQSVASYSRAMTQDPLWPQARLGRAMALLELGRLDEAVNEYRNVVQLGKATP
                     QGVLEFARLLVLSTLSKPPQERNWLEVEQWLDTLEKDDTTKTDAIILRAEILAAEKRL
                     QEAEQLLESHLSQDAKNDSLGLALVALKLQAGDAAEASRLLDQIEKQSGQTLAVLLSR
                     ARLAVRSGDDKRAKQELANIASAAKKLPAEEYQRLLGTLVGYAIQLSDFDNARRWAEE
                     VARLSPNNLKVRLLLFDLALYAQDMAVLSRVLDEIAQIEGQGPIWLYGQAIRLYLESK
                     GENRSLLQQALDYLSRAANLRPNWMRIPALQGLVFDLLGDTGSAINAYLRAVSLGDRS
                     VRTARRLVQLLYGQQRFLEADQIIRQLEQQQAPFSSELERLASSISLRLEDYERAVEL
                     ARRAVSGSSDYRDHVWFGTVQSVLGLRARAERREKEAQALLAEAEAAFRKGVELAPES
                     VEPWVALVQFYARIGENAKAEQATNEAKEKVKGGIGPLAMGQVYEVLGKLDEAEKYYQ
                     EALAIAPNDLLVVRQVTEFYLRTGRTESAVTQLNRIISGQVQASESDVSWGRRSIAMI
                     LAAQGGYDNLQKGLELIRQNRSAGRTPNIQDLRAEAILLTSHPQRKKRLEGRDLLEKV
                     VKESPQATPDDRFVLAQMYMADGDVVKAARHMRTLLATRGDDPRYLRAFVAFLLDQGQ
                     VAEAEIWQNRLEKVAPDAFPTLALRTRILTIQGRYDEAIQRLREFAEKETNNPPSDSP
                     SELSNDTPSEGSPGTNAEVSEEQLTKVQQLRWALAGERLEFLGRMLERSGKQSEANLF
                     YEE"
     gene            4233..>4663
                     /locus_tag="PKI05_04220"
     CDS             4233..>4663
                     /locus_tag="PKI05_04220"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOA51436.1"
                     /translation="MASGEVAANQGKGLTRELCAQYLSEGTFEQKIEAITSLVQLRES
                     GIDLILQALQDHDPRVRAKAITALAELAPLDAERVIPQLLVSLEQDDGFVDGQPNWIR
                     ASQAIGKYGDKVLPILEKLWQDTDLKKRMIFCIVCYELGPIA"
BASE COUNT         1025 a         1309 c         1185 g         1144 t
ORIGIN      
        1 cttcttcata gaagaggttc gcttccgatt gcttgcccga ccgttcgagc atccgcccca
       61 gaaattccaa cctctccccc gccagtgccc aacggagttg ctgaaccttt gtcaactgtt
      121 cttcagaaac ctccgcgtta gtacccggag aaccttctga gggagtgtcg ttcgacaact
      181 ctgacggcga atcactaggt ggattatttg tctccttttc tgcaaactcc cgcagccgtt
      241 gaatcgcctc atcgtatcgt ccctggatcg tgaggatccg ggtacgcaaa gccagtgtgg
      301 ggaaggcatc cggagcaact ttctctagcc ggttttgcca aatttctgct tccgccactt
      361 gtccttggtc gagcaaaaag gcaacaaatg ctctcaagta tcgggggtca tcaccccggg
      421 tggccaacag ggtccgcatg tgtctggctg ccttcacgac gtccccatcg gccatgtaca
      481 tctgagctaa gacaaaccgg tcgtcggggg ttgcttgcgg cgattctttc acgacttttt
      541 ccagcaggtc tcgtccttcc aatcgtttct ttctttgagg gtgggaagtc aaaaggattg
      601 cttcggctcg caaatcttgg atgttcggtg ttcgccctgc gcttcggttt tgtcggatca
      661 attccaggcc tttttgaaga ttgtcgtacc ccccttgggc ggccaagatc atagcgatac
      721 tacgacgtcc ccaagagaca tcactttcag aggcttggac ctgaccactg atgatccggt
      781 tcaattgggt gacggctgat tcggttcgcc ctgtccgcag gtagaactct gtcacctggc
      841 ggaccaccag taaatcgttt ggagcaatgg cgagggcctc ttgataatac ttctcggctt
      901 cgtccagttt gcctaaaact tcgtacactt gccccatcgc aagtggccca ataccaccct
      961 tcactttttc tttcgcttca tttgtggcct gctccgcttt tgcattctca cctattcggg
     1021 cgtagaactg cacaagggcg acccacggct caaccgattc cggcgccagc tcgacaccct
     1081 ttctaaacgc cgcttctgct tctgctaaca atgcttgcgc ttccttctca cgacgttccg
     1141 cacgcgcccg caatcccagc acgctttgca ctgttccaaa ccaaacgtga tctcggtaat
     1201 ctgacgagcc gcttaccgcc cgacgggcaa gttcgactgc ccgctcatag tcctccaaac
     1261 ggagcgaaat gctggaggca agccgctcta actcactgga aaagggtgct tgctgctgtt
     1321 ccaactggcg gatgatttgg tctgcttcaa ggaagcgctg ctgcccgtag agaagttgca
     1381 caagccgtcg tgccgtgcga acactccggt cacccagact aacggcacga agataggcat
     1441 tgattgccga tcccgtatca cccagcaaat cgaaaaccag gccttgcagg gcgggaatgc
     1501 gcatccagtt tggtcggagg ttcgccgccc gcgaaaggta atcgagggct tgctgcaaga
     1561 gactccggtt ctcgcctttc gattccaagt acaaacgaat ggcctgaccg tagagccaaa
     1621 ttggcccttg gccctcaatc tgggcaatct cgtcgagaac tcggctaaga acggccatgt
     1681 cctgagcata aagggccaag tcaaagagta gcaggcgaac cttcaagttg ttgggggaca
     1741 atcgggcgac ttcctctgcc cagcggcgag cattatcaaa atccgaaagt tggatcgcgt
     1801 aacctaccag cgttcccaat agacgttgat actcttccgc tggcaatttc ttggccgcag
     1861 acgcgatgtt ggctagctct tgtttcgccc gcttatcatc gccactgcgg acagcaagtc
     1921 gtgcccgcga taatagcacg gcaagggttt gtcccgattg cttttcaatc tgatcgagga
     1981 gtcgcgaggc ttcggccgca tcaccagctt gcagtttcaa ggcaacaagc gccaagccca
     2041 aggaatcgtt tttcgcatcc tggctcaaat ggctttctag aagttgttct gcttcttgta
     2101 agcgcttttc ggcggccaga atctcagcac gtaaaatgat cgcatctgtc ttcgtggtat
     2161 catctttttc caaggtatcc agccactgct ccacttccag ccaattacgt tcctggggag
     2221 gtttactgag cgtgctgaga acgagcaagc gcgcaaattc aagaacacct tgaggagttg
     2281 cctttcccaa ttgcacgacg ttgcggtatt cgttgacagc ctcatcgagc ctacctaact
     2341 ccaggagcgc catcgctcgt ccgaggcgtg cctgcggcca aagcgggtcc tgtgtcatgg
     2401 ctctcgagta agaggccaca ctttggtcag gattacccat ctgggcataa cagagcccga
     2461 gaagaaaatc cacttgctta agcaagtctg gcgaatctgt aacgtcgggc cgcaccgact
     2521 ccaacttacg agctgccaaa aaccactgtt ctttggcggt cagaagtcgc ccacgcaaga
     2581 attcaacttg ggcacgtgga taggaaaccg cctcgagttt ctcgatggtc tgctccgcat
     2641 ccgccaggcg aagggcatca atctgcatgc tggcgagcgc ccaatagagg ggacctttat
     2701 ccttcacttt ggaggctgcg tcttcgagcg tgttaattgc tgtgtctgca ttacctttgg
     2761 caaactgtgt ggctgcatag acggaatagg gatcgggtga ctccggggag gcggcgatcg
     2821 ctttttgagc aaactcctcc gcttggtcta actgccccaa ccgccgggca ctgtctgccg
     2881 cgaccaagag catctcaaga ttcgaaggat cttttttgtg cgcctcatca gcccatttca
     2941 atgctgcttc atagttctgg gcgttgtaaa aataggaagc cgctaccaaa taggcctcag
     3001 cggaatctcc gacagttaac accattttgg taatccattg atcaccttgc tcaggctgtt
     3061 tgaggtcgtg ccggtaggtg ccggctaact gagcgtatgc agggacatag tcctccttca
     3121 gctcgatgac gcgacggaaa gtggttgtcg cccgttcgta ctgacccagt cccctttgac
     3181 agcgtcccaa ttccgttagt agttctggat catccggata ctctttgaga aggtactgct
     3241 caatatgttc ctgggcgtct cgaaaccgtc ccatcgcagt gagaacctgc acgagttttc
     3301 gccgggtttc cagccggcca gggtcctgcc gcaagaccat ctccaagcga gtgaaggccg
     3361 gccagtaagc ctttagatcg gcaagtagac tcccatactc cgataaggcg tccaggtcat
     3421 tcgggaccaa atcgatatac cattgataat tcttgaccgc cacatcgaat cgttcttggg
     3481 ttttggcctc ccgagcacgg tccagaaaga cccttgcatt gcgccgggtt tggaatccgt
     3541 ggaggagata gccactaacg atcgcaagaa cgactgcacc caccaggatg acggcaagct
     3601 ttttgttgac ggttcgcgcc attcagatat cccccttaac cgttcgagct atccatagag
     3661 cgccccactc tttgccaaat tcaaccaaga cttctcaggg ttcagcgtag agacatgaca
     3721 gggccgaaaa atcttagcct attataactg gggcgtgtct ctgatcgatc acccgaaagg
     3781 gtggcttgat ttaccattct gcaactagga cttgactttg ttctaaccta cagatgctgc
     3841 acgggcacaa ggtggaaaac gagttcttaa gggaggcctg tccagcgaaa cagaattgcc
     3901 gaacattaca ttgagggttg atggaacgat caaacgggct gtgccaacga aaccaccact
     3961 tgtcattaca acacttatcc ttaagaattg ggttggtcga gttgaatcac gctgtcccct
     4021 tgcttaaaag gttttttatg tcactcttcc acctagtcaa gcgcttgttg tggtgcccgc
     4081 cgaggcactc atccccctca cagttaagcg gagccgccat cctctgtata acgttgatta
     4141 tttgttacac cccgctgaca cttccagttc tcttgggcga ggagccgagt gcctctgttg
     4201 gcggctcggc ttctggtagc caatcgaaag acgtggcttc cggcgaggta gctgcgaatc
     4261 agggcaaagg cttaactcgc gagctgtgtg cccaatatct ctcggaaggg acatttgaac
     4321 aaaagattga agccattacg tccttggttc aattgcgaga gtcggggatc gacctcattc
     4381 ttcaagccct tcaagatcat gatccgcggg ttcgtgcaaa ggcgatcacg gcccttgcag
     4441 aattggcgcc gcttgatgca gaacgcgtca tacctcaact gctggtgtcg cttgaacaag
     4501 atgatggatt tgtcgatggt cagcctaact ggatcagggc ctcccaggcc attggaaaat
     4561 acggtgacaa ggtcctgccc attttggaaa agctttggca ggataccgat cttaagaagc
     4621 gcatgatctt ctgtatcgtt tgctacgaat tgggaccgat tgc
//
LOCUS       DAOPTR010000154         4660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_14910_length_4660_cov_3.632356, whole genome shotgun sequence.
ACCESSION   DAOPTR010000154 DAOPTR010000000
VERSION     DAOPTR010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14910_length_4660_cov_3.632356"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="PKI05_04225"
     CDS             <1..787
                     /locus_tag="PKI05_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51437.1"
                     /translation="TIATQLLLKGANDPHSADAVLEQFLPEPLDTIDHLDVTVKARSL
                     SLKLECDAWLEQSNDNAQIVRLMREIPIEARLTVLEDLGTRLSELPPQVRKRLALSLA
                     HICEDLPKEWSSVPGPRRPQLGLAYSYVLMLSDNESAALSWLRQLAAEFPDAADIQEE
                     YALLLARQDRMDLRQEALERFRAIAQGTPEGSPRWFRAKYMTAWLLIQLGQPERALEM
                     IRVLEAIHPELGGDALRDRFRRLRALCENQITNSRPTSAGTAR"
     gene            complement(791..3598)
                     /locus_tag="PKI05_04230"
     CDS             complement(791..3598)
                     /locus_tag="PKI05_04230"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOA51438.1"
                     /translation="MRSLSVKTRRSVCHQRTWAFTCLVMLSALGPWTSLWTQAQGSSA
                     EKLTFYVAESGNDQWTGRLPEPSSDKNNGPWASLVPLAEVLQKLAQERPSAPVEVVIR
                     PGRYELSEPIILDLSKMSLGQLTIRAERSRTAFLSGGRRITHWVPASTIGDLPPQLPA
                     ESRPHVFVADLKALGIHDYGSPGGGGIELYYQDTPMTLARWPNEGFVKIADVINEQPF
                     KTHGIPGDKIGKFVYEGTRQDRWQHEKDPWVHGYWFWDWSDQRHAIQSIDPERHLITV
                     KPPYHHYGYRKGQWYYGFNLLCELDTPGEWYLDRENGRLYFRPPNSPKTVDELLAGQP
                     TVSCLTSLIEISNGRNVEISGLVLAHARGTAVRFVNCVDCQITDSHLYLLGGWGVTIQ
                     GGQGCRVSDCHLHLLGEGGVSATGGDRANLISAGHVIERNEIDHYGRIYPMYRAGVSI
                     SGVGQSVVHNHIHHAPHQAVSFSGNNHLIAFNEIDHVCLESNDAGAIYAGRDWTMRGT
                     QIVSNYFHDINGFENRGCMGVYLDDMFCGTVIRRNVFCRVTRAAFIGGGRDNLVENNI
                     FIECRPAVHVDARAMGWASYHVDTTMTERLKAMPYQSPLWQSAYPQLVNILQDEPAAP
                     KGNIIRHNVRVGGVWEHVEDKARPYLVMEKNLILPSEAISELFVDPQTENWQLKPDGK
                     VREELPEFESIDFEKIGLPRTGDGQSSEAGYRFIFVSQGEPTKEKQPPLPTRWEATIK
                     KMEDQDKEKAPPKDAVLFVGSSSIVRWDVAKWFPDLVTLNRGFGGSQIHEVTYYAERI
                     VIPYHPRLIVFYAGDNDIASGATPEEVLKRFQEFVTRVRASLPSTPIVFISIKPSLAR
                     WHLIESVRRANQLIQQYCRENPDLKLIFVDVDQAMIGPDGKPKPELFVKDGLHLSEDG
                     YRLWTELLRPVIDAQLKENRP"
     gene            complement(3712..>4660)
                     /locus_tag="PKI05_04235"
     CDS             complement(3712..>4660)
                     /locus_tag="PKI05_04235"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA51439.1"
                     /translation="VDVVEGAAMVAAARKYNRVVQINTQLRSTPHLIEARDRFIRENK
                     LGKIAHVEIYCYYHMRFRGTSPDTDPPPHLDWDFWCGPAPYRPYNPAIHPRRWRSFME
                     YGNGIVGDMCIHMLDMVRWMLDLGWPKRISSSGGILVQKDSIANIPDTQTAVFDFGDV
                     TVVWQHRTWGSTPNPDYPWGATFYGDQGTLHASVFRYDFIPQGQGEKVHQDVQYELEQ
                     YPEDKTEKDLERHVAPAIRRHIRNFLDAVDARTRPVADVEQGFISSASCILANLSLQL
                     GRTLTWDAEHLQVVGDPEANQRLARPYRAPWEHPDPSKV"
BASE COUNT         1072 a         1342 c         1180 g         1066 t
ORIGIN      
        1 gaccatcgcg acacagttgc tgctaaaagg cgcaaacgat cctcactcgg ccgacgcagt
       61 tctcgaacaa tttttgcccg aaccactcga tactattgat cacctcgatg tcacggtcaa
      121 ggcgagatcc ctcagcctta aattagaatg cgatgcctgg cttgagcagt cgaacgataa
      181 tgcacaaatt gttcgcctca tgcgtgaaat accgattgag gcccgtttga ccgttttgga
      241 ggatctcggg acgcggcttt cggagctccc gccccaagtt cggaagaggc tggcactctc
      301 cctcgcccac atctgcgagg acttgccaaa agagtggtcc tccgttcccg gaccccgtcg
      361 cccacagttg gggctggcct atagttacgt gttaatgcta tccgacaacg agtcagcggc
      421 cctctcatgg cttcgccaac ttgccgcaga gttccccgac gctgccgata ttcaagaaga
      481 atacgccctt ctcctggccc gacaagaccg aatggattta cggcaggaag ccttagagcg
      541 ctttcgcgcc attgcccagg gaacgcccga aggctcaccg cgctggtttc gcgctaagta
      601 catgacggcc tggcttctca ttcagcttgg ccagccggaa cgagctctcg agatgattcg
      661 tgttttggaa gcgattcatc cagaacttgg tggtgacgcg ctgcgtgacc gatttcgccg
      721 gctgcgggcc ctttgcgaaa accagatcac caattctcgt ccaacatcgg caggcaccgc
      781 ccgatagtaa tcagggccga ttttctttca attgggcatc aatgaccggt cgcagtaatt
      841 ccgtccatag gcgatatccg tcttcactca gatggaggcc gtccttgaca aacaactcgg
      901 gcttcggttt tccatctggc ccaatcatgg cttgatccac atcgacgaag attaacttga
      961 ggtccgggtt ttcccgacaa tactgctgaa tcaattgatt ggcccgacgg acggactcga
     1021 tgagatgcca acgcgccagg gacggtttga tggaaataaa aacaattggc gttgatggga
     1081 gactggcccg aacccttgtg acgaattctt ggaagcgttt caggacctct tcgggagttg
     1141 ccccgctggc aatgtcgtta tctcccgcat aaaagacaat cagccgcggg tgatacggga
     1201 tcacgatcct ctctgcgtaa taggtaacct catggatttg agagccacca aagccgcggt
     1261 tcaacgtaac taaatcggga aaccacttgg caacatccca gcgaacgatg ctcgaacttc
     1321 ccacaaaaag gacggcatcc ttaggcgggg ctttctcctt gtcttgatcc tccatcttct
     1381 tgatagtcgc ctcccagcgg gtgggcaaag gcggttgctt ctcctttgtg ggctcccctt
     1441 gcgatacgaa aataaatcga taccctgcct cgctcgattg tccatccccg gttctcggca
     1501 agccgatctt ttcaaaatca atggactcga attctggaag ctcttctctt actttgccat
     1561 cgggcttgag ctgccagttc tctgtctgcg ggtcgacaaa cagctcagag atggcctcac
     1621 tcgggagaat caggtttttc tccatcacca aataggggcg agccttgtct tcgacgtgct
     1681 cccaaactcc acccacacga acgttatggc gaataatgtt gcctttggga gccgccggct
     1741 catcctgaag aatgttgaca agctgaggat aagccgattg ccacagggga ctttgataag
     1801 gcatagcctt caatcgctcg gtcatcgtgg tgtccacgtg atacgatgcc catcccatag
     1861 cgcgggcatc cacatggacc gccgggcgac actcaataaa gatgttgttt tccaccaggt
     1921 tatcccggcc acccccgata aatgctgcac gggtcacgcg gcaaaaaaca ttcctgcgaa
     1981 tcaccgtccc acaaaacatg tcatccaagt aaacccccat gcagccacga ttctcaaaac
     2041 cattgatgtc gtggaagtaa ttagaaacga tttgcgtgcc gcgcatcgtc caatctcggc
     2101 ccgcgtagat cgctccggca tcgttcgatt ccaagcagac gtgatcgatc tcattgaagg
     2161 caatcaaatg attgttgcct gaaaagctta ctgcctggtg tggggcgtgg tgaatgtgat
     2221 tgtggaccac ggattgccca actccactaa tagacacgcc ggcacgatac atagggtaaa
     2281 tccgcccata atggtcaatc tcatttcgtt caataacatg gccggctgaa ataagatttg
     2341 cacgatcgcc gccagtcgcc gaaactccgc cttcccccaa cagatgcaga tggcaatcac
     2401 tcacgcgaca gccttgaccg ccctgaatcg tgactcccca gccgcctaac aagtagagat
     2461 gcgaatccgt tatctggcag tcaacgcaat tcacaaaccg gacagccgtt ccccgcgcgt
     2521 gagccaacac aaggcctgat atctctacgt tacgaccgtt cgaaatctct atcagcgaag
     2581 tcaaacagga taccgtaggc tgacccgcca acagttcatc caccgtcttt ggggaatttg
     2641 gtggccggaa atagagtctt ccgttctccc gatcgagata ccattccccc ggcgtgtcta
     2701 gttcacacag caggttaaat ccgtaatacc attggccttt gcgataaccg taatggtggt
     2761 aaggaggctt caccgtgata agatgccgtt ccggatcgat cgactgaatg gcgtgccgct
     2821 ggtctgacca atcccaaaac caataaccgt ggacccaggg atccttttcg tgttgccatc
     2881 gatcttggcg tgtcccctca tagacaaact ttccaatttt atcacccggg atgccatggg
     2941 ttttgaaggg ctgttcgttt ataacgtcag cgatctttac aaatccctca tttggccagc
     3001 gggccaaggt catcggagta tcctggtagt aaagctcgat ccccccaccg cccggcgatc
     3061 cgtagtcatg gatgcccaac gctttcagat ccgccacaaa tacgtggggc cgagactcgg
     3121 ccggcaattg aggtggcaaa tcaccaatgg tcgatgctgg aacccagtgg gtgatacgtc
     3181 gtccaccgct cagaaaggcc gttctcgatc gctccgcacg aatcgtcaat tggccgagcg
     3241 acatcttgga caagtcaagg ataatgggct cagaaagctc ataccggcct ggacgaatca
     3301 caacctcgac cggtgcggat ggtctctctt gggcgagctt ttgaagtacc tcggccagtg
     3361 gcaccaagga tgcccaggga ccattattct tgtccgaact cggttctggc aggcggcctg
     3421 tccattggtc gttgccgctt tccgccacat aaaaggtaag cttctctgcg ctgctaccct
     3481 gggcttgagt ccaaagggat gtccaaggtc caagggcaga aagcattaca aggcacgtaa
     3541 atgcccaggt tcgctggtga cacacgctcc gacgagtttt aaccgagagg cttctcatgg
     3601 catttttccc aaagactgaa accctgacca atagccaaga ccccagatct gaccacctta
     3661 tcatcacgtg gccctgggtt tttaataacg gttttgatag ctaaatcgga atcacacctt
     3721 tgaaggatcg ggatgttccc acggggcgcg gtagggtcga gccaatcgct gattggcctc
     3781 gggatccccg accacctgca agtgctctgc atcccaggtc agcgtgcgcc ccaattgcag
     3841 ggacaggttt gctaaaatac aggatgccga cgagataaag ccttgctcta cgtcggcgac
     3901 aggtcgcgtt cttgcatcga cagcatccag gaaattgcgg atgtgccgtc gaatcgccgg
     3961 ggcgacgtgc ctttctaggt ccttttcggt cttatcttca ggatactgtt ccaattcata
     4021 ttggacgtcc tggtgtacct tttcgccttg tccttgcggg atgaaatcgt aacgaaagac
     4081 gctggcatgc agtgttccct gatcgccgta gaaggtagcc ccccagggat agtcgggatt
     4141 gggcgtgcta ccccatgttc gatgctgcca aaccacggtc acgtcaccga aatcaaatac
     4201 tgcggtttgc gtatccggaa tgttggcaat actgtccttc tgaaccaaaa tcccaccaga
     4261 agacgatatc cgcttgggcc aacctaggtc gagcatccaa cggaccatgt cgagcatatg
     4321 gatgcacata tctcccacga tcccgttgcc gtactccata aatgatcgcc atcgccgcgg
     4381 atggatcgcc ggattgtagg ggcgatacgg agccggtcca caccagaagt cccaatcgag
     4441 atggggcggc gggtcggtat ctggcgaggt ccctcggaac cgcatgtggt agtagcaata
     4501 gatttccaca tgggcgatct tgcccaactt gttttcacga atgaagcgat cccttgcctc
     4561 gatcaagtgc ggcgtacttc gcagctgggt gttgatttga accactcggt tgtacttccg
     4621 ggcggcagcc accatcgccg ctccctcgac cacatcgacg
//
LOCUS       DAOPTR010000155         4658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_14915_length_4658_cov_2.956767, whole genome shotgun sequence.
ACCESSION   DAOPTR010000155 DAOPTR010000000
VERSION     DAOPTR010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14915_length_4658_cov_2.956767"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PKI05_04240"
     CDS             <1..362
                     /locus_tag="PKI05_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOA51440.1"
                     /translation="HRFDLTSCIGCERCARDCPVSCIYITKERVPGRKGFRVTSFVID
                     YTKCMFCAYCTENCPVDCIFMGSTYDLSCYSRDGCLVDFAKLPLEIAWGRASLNPTAV
                     ALSKSVLEPVDEGPGGA"
     gene            675..1262
                     /locus_tag="PKI05_04245"
     CDS             675..1262
                     /locus_tag="PKI05_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOA51441.1"
                     /translation="MSGVALVGYFTVFGALAVGLVFGALLLGKLFRPDNPTPIKKETY
                     ECGEPSVGSADIQFDIRFYVMALVFLIFDVEVALFFPWATVFGKATHVASISVDSASR
                     RERLAELGITEATLPPLSGPKRPATLSNGISVGQQAAGPTGLPPVEELSAWGRRLATL
                     ALCDILVFFGVLMVGFAYVWCRGDLTWIRAVPHRA"
     gene            1358..1888
                     /locus_tag="PKI05_04250"
     CDS             1358..1888
                     /locus_tag="PKI05_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOA51442.1"
                     /translation="MSIEEIFQHLQEKFGEAVRPGDFTSIDPWIEVGPEAIADVCRFL
                     RDNPALRFEMLHCITAVDYLEVDEKKAAKASFVPHLELIYHLSSLTLRHRVTLKVMLP
                     RWRDDHPGQLPRVPTVSNVWKTALWHEREVYDLSGVEFVGHPDLRRILCPEDWVGHPL
                     RKDYQMPIEYHGIRAR"
     gene            2014..3228
                     /locus_tag="PKI05_04255"
     CDS             2014..3228
                     /locus_tag="PKI05_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOA51443.1"
                     /translation="MNVIPGVDPEDFSVTSETEVRTEELLINMGPQHPSTHGVLRVVL
                     RTDGELVLEAIPHIGYLHRCAEKIGEKVTPRQFIPYTDRMDYLAGMNMNLGWALTVEK
                     LLGLEVPEKARHLRVLIVELNRIASHLVAAGTYGLDLGSFTPFVYCFREREKILNLFE
                     AVCGARLTYSYITVGGLMADLPSGWLDQCEAFLDQFEPVIDDLHDLLSVNHIFVKRTA
                     GVGVLSRELAVDYGCTGPVLRGSGVDWDLRRDGDPFYTRMYRDYDFEVAVCRDGKYPQ
                     PSPPVPTEAVLGDCWHRFFVRMVEVVQSVRLVRQAIEKYRLAEGDIGQPIKLNQKLPP
                     GEVYLETECPKGQMGFMIVSLGEAIPWRVRARSSSFCNLSVLPELCRGCLVADVPAIV
                     GSLDIVLGEVDR"
     gene            3296..4471
                     /gene="nuoH"
                     /locus_tag="PKI05_04260"
     CDS             3296..4471
                     /gene="nuoH"
                     /locus_tag="PKI05_04260"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOA51444.1"
                     /translation="MAQWLSDWLTLSPQWEWVAYLTVALLQAVILLALVMTAVALATW
                     AERKVAGRIQDRLGPTRVGGRFGWLQPLADGLKLFTKEDLIPANADAVLFRLAPYLSF
                     APVFGVFMALPFSDGWVAHHFNTAAFFILAVLGLEVFGIILGGYASGSKWSLLGGMRE
                     AAQVVSYEVPLAFSAMLPILLAGTMDLVAIGEAQRGWFWNWFVFHDPFTFVAFWIYVI
                     CAVAGTNRAPFDLAEAESELVAGFHTEYSGFRWSVFYMGEYAAMAAVCGLGVVLFLGG
                     WNGPIPVAEWLFGPASSTSPIVNFLSQLLGAVNFIIKCIIGVVFMISLRWTLPRPRID
                     QVMSMCWKYCFPLAALAFLGATLWVLMLPDGLLGQGHPKASTCEQSVMVTVERIETP"
     gene            4468..>4658
                     /locus_tag="PKI05_04265"
     CDS             4468..>4658
                     /locus_tag="PKI05_04265"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOA51445.1"
                     /translation="MTGPDGMAILFWLFAIVTCGGALVVVLSRHIVRAAVGLILSLFG
                     VAMLFLLAGAEFVGAMQLM"
BASE COUNT          932 a         1144 c         1358 g         1224 t
ORIGIN      
        1 tccaccgttt tgacctgact tcctgcatcg gctgtgaacg ttgtgctcgg gattgtcctg
       61 tcagttgtat ctacataacc aaagagcgtg tgccaggcag aaaggggttc cgggtaacct
      121 catttgtcat cgactacacc aagtgcatgt tttgtgcata ctgcacggag aactgcccgg
      181 tggattgcat cttcatggga tcaacgtacg atctgagttg ttattcccgc gacggttgtc
      241 tggtggattt cgcgaaactc cctctggaaa tcgcttgggg acgtgccagc ctgaatccaa
      301 cggcggtggc cctgtccaag tcggtgctcg aacctgtgga tgagggtccc ggaggtgcct
      361 gagctttcga gcggttgata ttgtcaaaag gtcgaacctg gtttagggtg aaggcttaca
      421 tcctttgctt ttgactgccg ggcaaatcga cgatctggcg aattctgcgg aaaatcacac
      481 gagcatggaa tcaccgttta ccgtgaaaag gtgtgcggat cattcacaaa ccaatgcgcc
      541 gatgcgacca accgtttgcg gcctgcggtg atgacggaca ggcgcacttc ggcttatacc
      601 ggtgaatttt tggcaactta gcgattggtg acgcaaaaaa gaagtcccaa gaaaataaag
      661 gtgaccgaga acaaatgtct ggcgttgctt tggttgggta cttcacagtg tttggagccc
      721 tggccgtagg gctagtgttt ggagccttgc ttttgggcaa gctctttcgg ccagataatc
      781 cgacacccat caaaaaagag acctacgaat gcggtgaacc gtccgtaggg tccgcggata
      841 ttcagttcga tatccgcttc tatgtgatgg cactggtctt cttgatcttt gacgtggaag
      901 tggccttgtt ttttccctgg gccaccgtct ttgggaaagc gacgcatgtt gcgtcgatta
      961 gcgtcgattc tgcgagtcgt cgcgaacggc tggccgaatt gggaattacg gaagcgactt
     1021 taccaccgct cagtggtccg aagcgcccag cgacgttgtc caatggtata tcagtaggtc
     1081 aacaagccgc tggtccgaca ggtttaccac ccgttgagga gctttccgct tggggcaggc
     1141 ggttggcgac actggcactg tgcgatatct tagtcttttt tggtgttttg atggttggtt
     1201 ttgcctatgt ttggtgtcgt ggcgatttga cgtggatacg ggcggtgcct caccgggcct
     1261 gagcagcgat ttcccgtacc aacaaatttt cgcaagctgg aacggtcgtg cccgggtggc
     1321 agtctgatca tcaataagtg agcatatacc gggaaaaatg tccatcgagg agattttcca
     1381 acaccttcaa gagaaatttg gggaagccgt ccgaccaggc gacttcactt cgattgaccc
     1441 gtggattgag gttggtcccg aagcgattgc ggacgtttgt cgttttttgc gcgataatcc
     1501 ggccttgcgg ttcgagatgc tccattgcat tacggccgtc gattatttgg aagtggacga
     1561 aaaaaaggct gccaaagcct cctttgtccc ccatcttgaa ttgatttatc acctctcttc
     1621 gttgacgtta cgtcaccgag tgacgctgaa ggtgatgtta ccgcggtgga gagacgatca
     1681 tcccggccag cttccccgcg ttcccacagt cagcaatgtt tggaaaaccg ccctttggca
     1741 tgagcgggaa gtgtatgatc tttcaggcgt tgagttcgtg ggccaccccg atttgcgacg
     1801 aatcctctgc ccggaggatt gggtaggaca tccgctgcgg aaggactatc agatgccaat
     1861 cgaataccac gggattcgtg ctcgctagac catggagtgg gttcagtacc atagtagtgt
     1921 tccatatcgt gattcgatgg gactccttcc ccaaggagcc accccctctt tgagactgcc
     1981 agacataaat atgacagttg ttacgactac aatatgaacg tgataccagg agtcgacccg
     2041 gaagatttct cggtcacctc agaaacagag gtgaggaccg aggaattgct gatcaatatg
     2101 gggccccagc accccagtac gcatggggtc ttacgggtcg tcctccgtac cgacggcgag
     2161 cttgtcctcg aagcgattcc acacatcggt tatcttcacc ggtgtgccga aaagatcggc
     2221 gaaaaggtga cgcctcggca gtttattccc tatacggacc gaatggacta cctggccggg
     2281 atgaacatga atctcggctg ggccttgacg gtggagaaac tactgggtct ggaagtgcca
     2341 gaaaaggcta ggcatctacg tgtcttgatc gtcgaactca ataggattgc cagccacctt
     2401 gtggcggcgg ggacgtacgg attagacctg ggcagtttca ctccattcgt atattgcttt
     2461 cgtgaacggg aaaagatctt gaatctattc gaggccgtat gcggcgcgcg tctgacgtat
     2521 agttacatca ctgtgggtgg ccttatggct gacctgccaa gcggttggct ggaccagtgt
     2581 gaagcgtttc tcgatcaatt tgaacctgtg attgacgacc ttcacgatct gctttcggtc
     2641 aatcacattt tcgtcaagcg aacggccgga gtgggagtcc tttcgcgaga gttggccgtc
     2701 gattatggtt gcaccggacc ggtattgcgc ggaagcggtg tggattggga tttgcgtcgc
     2761 gacggtgacc cattttacac gcgcatgtat cgtgattatg acttcgaggt cgccgtctgt
     2821 cgcgacggga aatatcctca accctcgcca ccagtcccga cagaggcggt tctcggcgat
     2881 tgctggcatc gcttttttgt caggatggtc gaagtggtcc aatctgtgcg actggtccgg
     2941 caggcgatcg agaagtaccg actcgcggaa ggcgatattg gacagcccat caagttgaac
     3001 caaaagctcc cgcctgggga ggtctatctc gagaccgaat gccccaaagg ccaaatggga
     3061 ttcatgattg tgagtctggg tgaagccatc ccttggcgtg ttcgtgcgcg gagcagtagc
     3121 ttctgtaatc tttcggtgtt gcccgaattg tgccggggct gtttggttgc cgacgtcccg
     3181 gccatcgttg gctcactcga tatcgtcttg ggcgaggtgg atcgttagtt cggccgaagg
     3241 tggggtttca ctcagagaag ttgtacccga aaagaccagg gactctggaa aaacgatggc
     3301 tcagtggttg tcagactggt taacgctaag cccgcaatgg gagtgggtcg cctatttgac
     3361 cgtggcgctg ctgcaagccg tcattttgct ggctcttgtg atgacggccg tcgccttggc
     3421 aacctgggcg gagcgaaaag ttgcgggccg aattcaagat cgtctcggcc cgacacgtgt
     3481 gggaggccgt tttggatggc tgcagcccct ggccgacggg ctcaaacttt ttacgaaaga
     3541 agacttaatc cccgcgaacg ccgatgctgt gctttttcga ttggcgccct acttgagttt
     3601 cgcaccagtt tttggcgttt ttatggcact gccgttctct gatggttggg tcgcccatca
     3661 tttcaatacc gcggcattct tcatcctcgc tgtgctcggg ctcgaggtct ttggcattat
     3721 tctggggggg tatgcctcgg gctcaaaatg gtccctattg ggcggaatgc gagaggctgc
     3781 acaagttgtt agctacgagg tccctctcgc cttttccgcc atgctaccta ttctcttagc
     3841 gggcacgatg gatcttgtgg ctattgggga ggctcagcgc ggctggtttt ggaactggtt
     3901 cgtgttccat gatcctttta catttgtggc cttttggatc tatgtgattt gcgccgtggc
     3961 cgggacaaat cgcgcgccct ttgatttggc tgaagccgaa agcgaactcg tcgccggttt
     4021 ccataccgag tactccggtt ttcgttggag cgtcttttac atgggcgaat atgcggcgat
     4081 ggcagccgtc tgcggtttag gagttgtcct ttttttgggt ggctggaatg gtcccatacc
     4141 ggtggcggag tggctttttg gcccagccag ttcgacgagt ccaattgtca atttcctcag
     4201 ccaattgttg ggggcggtca atttcatcat taaatgtatc atcggcgtcg ttttcatgat
     4261 ctcactgcga tggacattgc cacgtccgcg aatcgaccag gtcatgtcta tgtgctggaa
     4321 gtactgcttt ccgctggcag ccctagcatt cctcggggca actctctggg ttttaatgtt
     4381 acctgacggt cttttgggcc aaggacatcc taaggcttcg acatgtgagc aatcggtaat
     4441 ggtcacggtc gaaaggattg agacaccatg acagggcctg acggaatggc aatccttttt
     4501 tggttattcg caatcgtcac ctgcggaggt gccctggttg tggtattgag tcgccacatc
     4561 gttcgggcag ccgtggggct tatcttgtct cttttcggcg tggcgatgct gtttttactc
     4621 gctggggcag aatttgtggg agcaatgcaa ctcatgat
//
LOCUS       DAOPTR010000156         4652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_14936_length_4652_cov_3.735480, whole genome shotgun sequence.
ACCESSION   DAOPTR010000156 DAOPTR010000000
VERSION     DAOPTR010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14936_length_4652_cov_3.735480"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..94)
                     /locus_tag="PKI05_04270"
     CDS             complement(<1..94)
                     /locus_tag="PKI05_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008672678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOA51446.1"
                     /translation="MAHPDDAEFCCGGTLARLRELGWEVHIASLA"
     gene            complement(268..1026)
                     /locus_tag="PKI05_04275"
     CDS             complement(268..1026)
                     /locus_tag="PKI05_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOA51447.1"
                     /translation="MGLWTDLKILYRMVFHPGRGQDHASRMESFYAPQAAYYDSFRRR
                     LLKGRDDLWQAIGVPENGLWVDLGAGTGANAEYFGDNISRLRKVILVDLSPSLLAVAQ
                     ERIKSHGWTNVETQVADATVFQPPEPADVVTFSYSLTMIPDWFAAIDNAWRMLKPGGK
                     IGVVDFYVSRKHPAPGMKRHGACARLFWTFWFNYDDVFPSPDHIPFLQRRFRQILLEE
                     RRAKVPYLPLARAPYYLFVGEKTEESPAPPYFSA"
     gene            complement(1092..2285)
                     /locus_tag="PKI05_04280"
     CDS             complement(1092..2285)
                     /locus_tag="PKI05_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtaA family protein"
                     /protein_id="HOA51448.1"
                     /translation="MSVGEWVSSKVFTLVHRHNLVYNTCWEDPRLDRVALNIGPEDTI
                     LVITSAGCNALDYALLGPKHIYAVDMNPRQNALLELKMAGIRRLDFPTFFQIFGEGRY
                     RGIEEVYRSSLRRELSPFARRYWDKHIKRFFGRKRSFYFRGTAGTFARLMNTYITKVA
                     RVRPWVEKILNAESLSEQREIYEKHLKEQVWTPLLRFLLSRDAMLSLVGVPRAQRQQI
                     ESQYEGGVAQYVQDCVDAVFGKLPLRDNYFWRVYITGSYTRECCPEYLKEENFYLLKN
                     GLVDRISTHTNTVEGFLVENDVTISRYVLLDHMDWLSGRFFPLLEAEWQAIFDRAAPG
                     ARAIWRSGGLRTDFVDRVRVQLGGRTVALGEQLTYHRELAAELHAKCRVHTYGSFYIA
                     DLNVP"
     gene            2896..3369
                     /locus_tag="PKI05_04285"
     CDS             2896..3369
                     /locus_tag="PKI05_04285"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HOA51449.1"
                     /translation="MSDSPDSRFADGQTEPGGQRNQQQRIIVDEDWKAQVEREKREAA
                     EKKDSQAGLDPPLPPPTLTALSSSLAMQAMAALGLLPDPLSGKLEVRLNRARHLIDTV
                     NLLLEKTKGNISEEESRAMEEMLHELRLTYVQVQARLASQPTSEKEKSPSEGGSS"
     gene            complement(3641..>4652)
                     /locus_tag="PKI05_04290"
     CDS             complement(3641..>4652)
                     /locus_tag="PKI05_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOA51450.1"
                     /translation="VEKMEERYALLARAGVRHLSVYNQLGEEELMERIRPESEEERAQ
                     IPTHMPYIVIIADEMADLMMTAAKEVETHIIRLAQKSRAVGIHLVLATQKPTVDVITG
                     LIKSNLPARICFEVASRVDSRVVLDEMGAEKLLGNGDMLFLRPGTSTLIRGQGTYVSD
                     DEINRVIDAVAVHEPEFVQELVQLKPSQPGDPNKPGLAARDELYEAAVDIVIREGRGS
                     VSLLQRALGIGYGRAARLIDYMEEDGIVGPYNGSQAREVLMSLEQWEAMRQNNGPATS
                     QPASSRPATHSAPSNAGRSHRIVLVSETGEDTPPNGPSTSFGPLPPRPESTPPAQPPT
                     NV"
BASE COUNT         1070 a         1294 c         1297 g          991 t
ORIGIN      
        1 gggcaaggct tgcgatgtgg acctcccaac cgagttcccg caagcgagcc aacgttccac
       61 cgcagcaaaa ttcagcatca tcgggatggg ccatgagagt taacgccacc cgattgattt
      121 gatgcgacat atggcgcctc cgaatccacc aataagcagc attaagagaa caagccgact
      181 gggaaaacag tcattacgtt agcgtaaaga cacccctcac ggcaagtccc gccacaagga
      241 ggagttggtg ttgaggaaaa tgccacgcta tgcagaaaaa taaggcggcg cgggcgactc
      301 ctcggttttt tcgccgacaa acaaatagta tggtgcccga gctagcggca aataagggac
      361 tttggctcgc cgttcctcaa gcagaatctg ccggaaccgt cgttgaagaa aggggatgtg
      421 gtccggcgat ggaaaaacgt cgtcgtagtt aaaccagaac gtccagaaca gtcgggcaca
      481 tgccccgtga cgtttcattc caggtgcggg atgcttgcgg gagacgtaaa aatcgacgac
      541 gccgatcttc cccccgggtt tgagcatccg ccaggcgttg tcgatagcgg caaaccaatc
      601 cggaatcatt gtcaaagaat aggagaacgt gacaacgtcg gcaggctccg gtggttgaaa
      661 gaccgtagcg tcggccacct gggtttccac atttgtccag ccgtgactct taattcgctc
      721 ttgggctacg gcaagtagcg acggcgagag atcgaccagg atgaccttcc gcaaacgaga
      781 aatgttgtcg ccaaaatact ctgcattagc cccggtgccg gcccccaaat ccacccatag
      841 tccgttctcc ggtacgccaa tggcctgcca aaggtcgtcc cgacccttga gcagtcgccg
      901 tcgaaagctg tcgtagtagg ccgcttgagg ggcgtaaaaa ctttccatcc gcgaggcatg
      961 atcctgccca cgccctggat gaaagaccat gcgatagagg attttcagat cggtccaaag
     1021 acccatggga tgaatgaacc tccttgagta gctcagacgc agcccttagt ctgttgctga
     1081 cgatcgcgcg tttaggggac gttcaaatcg gcgatgtaga aacttccata agtgtgaacg
     1141 cggcacttgg cgtgtaattc ggcggcgagt tcccgatggt acgtcaattg ctcacctaac
     1201 gcgaccgtac gcccgcccaa ctgaacccga acgcgatcga caaagtcagt ccgcaaacca
     1261 ccactccgcc agattgcgcg agcgccgggc gcagcccgat caaaaatggc ctgccattcg
     1321 gcttccagaa gcgggaagaa acggcccgac agccaatcca tgtggtcgag gaggacgtag
     1381 cggctgatgg ttacgtcgtt ctccacgagg aaaccttcca ctgtgttggt gtgagtcgag
     1441 atacgatcga ccaaaccatt ctttagcagg tagaagttct cttccttgag gtattcggga
     1501 cagcattccc gggtataact ccctgtgatg tacacacgcc aaaaatagtt gtctcgcagc
     1561 gggagcttac cgaagacggc atccacgcag tcttgcacgt attgggcaac acctccttcg
     1621 tactgggact cgatttgttg tcgctgggct cgcgggacgc ccaccagcga aagcatcgcg
     1681 tcccgactga ggagaaaccg cagcaaggga gtccagacct gttccttgag gtgcttttcg
     1741 taaatttctc gctgttcact aagagattcg gcgttgagaa tcttctccac ccatgggcgg
     1801 actcgagcca ccttagtgat gtacgtattc atgagccggg caaaagtgcc tgccgttccc
     1861 cggaagtaaa acgatcgctt acgaccgaag aatcgcttga tgtgcttgtc ccaatagcgg
     1921 cgagcaaagg ggctgagttc acgccgcaac gacgaacgat agacttcctc gatgccgcga
     1981 taacgacctt caccgaaaat ctggaagaaa gttggaaaat ccaagcggcg aatgcccgcc
     2041 atttttagtt ccaacagggc attctgccgg ggattcatat ccaccgcgta aatatgcttg
     2101 ggcccaagga gggcgtagtc taaggcgttg cagcctgccg aagtgatgac caagatggta
     2161 tcctcgggtc cgatattcag cgccacccga tccagacgag ggtcttccca gcatgtgtta
     2221 taaaccagat tatggcgatg aacgagagtg aagaccttac tgctgaccca ttctccgacc
     2281 gacatgctcg cggctcctcg tctctattga tgaggtgtgc cgtttcccga tggaggaacc
     2341 cgcagtctct ttcgctttcc ccgcactcgg aaaccggcgt tctgtggaac caatcctccc
     2401 cccacgattt ggtaacataa cccccaatat acggcaaatc tggcgaaata caagcttgga
     2461 ggggattttc gccaccgcca gcagcgaagc cacttggcct tcctaattct cgtgaggaaa
     2521 aagacacccg cgcagatcgg atgtcgcccg tgactctgag acgtaacaag cccggcgaca
     2581 aacgttgctc ctagcgcagc attccaatgg ctcagcacca ccgctatggg ctggacttgt
     2641 gcctgtccga tgtgacggag gggcacttgc cgttgtggcc tcgctcatgc agggccacgc
     2701 gctgtggtgg ccgttttcac tatttccaat gggaagaaat ttcccgcaaa gggctcaatc
     2761 tcgaagggtt ttttggtttt ttgtcggcca ttcttgactt gcccgctcaa accgccaaac
     2821 tgataaagaa tctccgggga acttacgtgt gctcttggct aactcgtgag gtttccgata
     2881 tctggaggat tgtacatgag cgactcaccg gacagtcgct ttgcggatgg tcaaacagag
     2941 ccgggcggcc agcgaaatca acagcagcgg attattgtgg atgaggactg gaaagcccag
     3001 gttgagcgag aaaagcggga agcggctgag aaaaaggatt ctcaagccgg ccttgatccg
     3061 ccattgccgc cgccaacatt gaccgcactc agcagcagcc tcgccatgca ggccatggcg
     3121 gctttggggc tccttccaga tcccctcagc ggtaagctgg aagtgcgact taatcgggcg
     3181 cgacacctga ttgatacggt caacctcctc ttggaaaaga cgaagggtaa catttcggag
     3241 gaggagtcgc gggccatgga ggagatgctc cacgagttgc ggctcaccta tgtgcaggtc
     3301 caggctcggt tagcaagcca gcccacttct gaaaaggaga aatcgccgag cgaaggtggg
     3361 tcgtcctgac ctcgaccagc ccccgcgttc atattgaagt ctctttaact ccgcatgaat
     3421 ttaaaggccg attggcacgg cttgaattag taattgcggc ttcacttggc gttcctaata
     3481 gccgcctcgt aacagcctat tccaaaaggt ccccctctcc ctccgggaga gggtcggggt
     3541 gagggcaagc gaacgtcgga atcgattgag aaaaccggca gtaattcgag ccacggcgcg
     3601 tctctcgccg aacgggtgag aagagattat gggataggtt ctaaacgttg gttggaggtt
     3661 gggctggcgg ggttgactca ggacgcggtg gcaacggccc aaatgaggta gatggcccgt
     3721 ttgggggggt atcttcccct gtctcagaga ccaggacaat ccgatgggag cgaccagcat
     3781 tactcggtgc ggaatgggtt gcaggccgag atgatgcagg ttgagatgtc gccggtccgt
     3841 tgttctgtcg catggcctcc cattgttcaa gggacatgag gacttctcga gcttgtgagc
     3901 cgttgtacgg cccaacgatg ccgtcttcct ccatgtagtc gatcaagcgg gcagccctgc
     3961 cgtaaccaat tcccagggcc cgttgcaaca aagagacact cccgcggcct tcccggataa
     4021 caatgtccac ggccgcctca taaagttcat cacgggcggc cagtcccggt ttgtttggat
     4081 caccgggttg ggaaggcttc agttggacaa gctcctggac aaattccggc tcgtgcacgg
     4141 caacggcatc aatgacgcga ttgatttcgt cgtcactgac gtaggtacct tggccgcgaa
     4201 tcagtgtgct tgtccccgga cgtaggaaga gcatatcgcc attgccgagg agtttttcgg
     4261 cacccatttc gtccagcacg acgcggctat ccacgcggct ggctacctcg aagcagatac
     4321 gggcaggcag gttggactta atcaggcccg tgatcacatc aacggtaggt ttttgcgtag
     4381 ccaaaaccag gtgaatccct accgcccggc tcttttgggc aaggcggatg atatgggttt
     4441 cgacctcctt ggctgccgtc atcatgaggt cggccatctc gtcggcaatg atcacgatat
     4501 acggcatatg ggtcgggatt tgtgcacgtt cttcctcact ttcaggccga atcctctcca
     4561 tcaactcctc ttcgccaagt tggttataca cactgaggtg ccgcacgccc gctcgcgcca
     4621 acagggcgta ccgttcttcc attttttcca cc
//
LOCUS       DAOPTR010000157         4649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_14955_length_4649_cov_3.120374, whole genome shotgun sequence.
ACCESSION   DAOPTR010000157 DAOPTR010000000
VERSION     DAOPTR010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14955_length_4649_cov_3.120374"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PKI05_04295"
     CDS             complement(<1..830)
                     /locus_tag="PKI05_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOA51451.1"
                     /translation="MVWSSFRGWILVCLVVCNQAGLALYLRASESSNARVVPPWPPTE
                     TRLRVIIDTDAATEVDDQHALALALVSNDRFKIEGIVAAHFGDKGGPQGIERSYQEVL
                     TILEKAGLKGQIPVKKGSHPFTYSQVPPPSEGVDFIIERAMADDPAPLWVISLGPCTD
                     IAAAWLKEPRIKDRVIAFWHGRTEWPTKAWNFNAYNDLKAVRILFSSDLPFILFDTGT
                     YLTCPMEESEKRIRPHGALGAYLHEIRFRSPAWQSPNKGLFDLGDIAALVDPSLVQFE
                     V"
     gene            complement(1018..1596)
                     /locus_tag="PKI05_04300"
     CDS             complement(1018..1596)
                     /locus_tag="PKI05_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51452.1"
                     /translation="MEPLTLEKLLELLKSDDPLVRRDAWLKAGPLGGDALPSLADLAA
                     KADLEISRAANRAMWQIVRYAGAPGRETERQGVVEALGELVVDTRLPVQLRRDVVWML
                     SEIIADEEIDESIASSLLKDPELQEDVRMALQRVAGPKAVALLKAALNQAEGDFKAAL
                     ACSLRKCHVAIDDPPCPKLVPQKQTRVKPVGR"
     gene            complement(1683..3062)
                     /locus_tag="PKI05_04305"
     CDS             complement(1683..3062)
                     /locus_tag="PKI05_04305"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA51453.1"
                     /translation="MGQRNVDGPRSQRLGRRRFLQTSVLAATASGVGVPEIIPRRAMA
                     WEGQPGANERITVAHIGMGGRGKGLYREMAGLRAAGLCDHVAVCDIDEKRLEEASRLV
                     GPQADVYRDYRYILERKDVDAVVIATPDHWHAVQFVHAAECGKHIYCEKPACCTIEEG
                     KAMVAAARKAKIASQIGSQGRSQPEAYLAHRYLVNGVIGRVYRVDCWHYPSPVDTSHT
                     PDSDPPPELDWDLWLGPLRWRPFNKRYLHGVFRWLLESGGGQIRDRGAHVMSCAMWWM
                     GADGTGPVEVEATGTPPEEGLWDSCVEMNVTYTFKNPDWILTWNQPGEPVPPEERTGQ
                     EPGGKIVRPGYGAVYRGERGTMVHWGGDGGTWVEKKVREWVPPPGAKDVYKSPGHFED
                     WFEGIKTGKKTIMDVEAGVGVAFLCILGNLSYMLGRKLHWDPVKQEIIGDEQARRLMV
                     RPQRHPYHL"
     gene            3605..4255
                     /locus_tag="PKI05_04310"
     CDS             3605..4255
                     /locus_tag="PKI05_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOA51454.1"
                     /translation="MDTAPRLEWLNHGAPRAEWSDEDLVREYQQTGEPRAFEELVRRY
                     ERELYNYLCQYLGDAEMAEDAFQATFLQVHRKCSQFDSGRRFRPWLYAVATNQAIDAQ
                     RRNRRHRLVSLDQQSRMYTESHDLGCLIDLVEGQEMDPEEHVELNERKEDVRQAVDQL
                     PESLRQVLLLVYYQGMKYREAAEVLNIPVGTVKSRLHTAIRRLEYHLKLPRSPSHA"
     gene            4248..>4649
                     /locus_tag="PKI05_04315"
     CDS             4248..>4649
                     /locus_tag="PKI05_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51455.1"
                     /translation="MPDCSPAQLIGYLLNALDEEEREEIEQELVRCPELRKKLQRLKR
                     RLAVLELGRREIAPPPGLADRTCQFVFQSCGCLAIRHTTKATSPALPASRDVAGFRLS
                     PLSDYLGGHGANWSFADLVVSALVVLIVVSLL"
BASE COUNT         1017 a         1372 c         1278 g          982 t
ORIGIN      
        1 acttcgaact gcacaaggga gggatcgacc agggctgcga tgtcacccaa gtcaaaaaga
       61 cctttgttgg gcgattgcca ggccggcgag cgaaaacgga tctcgtgcag gtacgcgccc
      121 aaggcgccgt gcggtcgaat acgtttttct gattcttcca tgggacacgt gagatatgtg
      181 cccgtgtcga aaagaatgaa cggcagatcg gacgaaaaga gaatccgcac ggctttaagg
      241 tcgttatagg cgttgaaatt ccaggccttg gtcggccact cggtccgtcc atgccagaag
      301 gcgatgacac gatctttgat gcgtggctcc ttgagccaag ccgcggcgat gtcggtgcag
      361 ggacctaacg atatgaccca aagcggggcc gggtcgtctg ccatcgcccg ttcgataatg
      421 aaatcgacac cttccgaggg tggtggaacc tggctgtaag taaacggatg tgagcctttt
      481 ttgacgggaa tttgaccttt cagcccggcc ttttccaata tggtcaggac ctcttggtag
      541 gagcgttcaa tgccctgggg acctcccttg tcgccaaaat gagccgccac aatgccttcg
      601 atcttgaacc gatcgtttga caccagtgcc agggctaggg cgtgttggtc gtccacctcg
      661 gtggcggcat ccgtatcaat gatcacccgc aggcgggtct ccgtcggcgg ccacggtggc
      721 acaacgcgcg cgtttgacga ttcacttgcc cttaggtaga gggccaggcc ggcttggttg
      781 cagacgacaa ggcataccag gatccaacca cgaaaggaac tccaaaccat gaagtcgctc
      841 cttgagggat tcctcgtgtc gattgttggg aagtcaaaat tcttcgtttt gaaaacgccc
      901 accgcgccta tcgagggttt cgattaacac cctcggtgga tttaagtcct attctgtttg
      961 gcgaagcccc aaagaccatt ctcgaccggg aacaacccat gccgctggtc aatccattca
     1021 ccgaccgacc ggcttgaccc gagtttgctt ttgaggcacc agcttcgggc agggtggatc
     1081 atcgatggcc acgtgacact tgcggagtga gcacgcgagg gcagccttga agtcgccttc
     1141 ggcctgattt agggcggcct tgagcaaggc aacggccttg ggaccggcaa cacgctgcaa
     1201 ggccatgcgg acgtcttctt gcagttccgg atccttcagt aatgaggagg cgatcgattc
     1261 gtcgatctct tcgtccgcaa tgatctccga gagcatccag accacgtcgc gccgaagctg
     1321 cacggggagc cgggtatcga caaccaattc gccgagtgcc tctacgacac cctgtcgttc
     1381 cgtttcccga ccgggggccc cggcatagcg gacaatctgc cacatggcac ggttggcggc
     1441 acggctgatt tccaggtccg cctttgccgc gagatcggcc aggctcggca aagcatcacc
     1501 acccaatggc ccggccttga gccatgcatc ccggcgaacc aagggatcat ccgacttcag
     1561 caattcaagg agtttttcca gggttaaggg ttccatagct gtatccttta tatgcgcaaa
     1621 cgtgtctgat ttcgaaaatg acctcgcact caatggccaa gagtcaaaaa gcctccgtca
     1681 cattacaagt gataaggatg ccgctgtggc cgaaccatga gccggcgggc ctgttcatcg
     1741 ccgataatct cctgtttgac cgggtcccag tggagttttc gccccagcat gtaagagagg
     1801 tttccgagga tacacaaaaa ggccacgcca accccggcct cgacatccat gatggtcttc
     1861 ttgccggtct tgataccctc aaaccagtct tcgaagtgac cagggctttt atacacgtcc
     1921 ttggccccgg gcggtgggac ccattcgcgg accttctttt ccacccaggt tccgccgtcg
     1981 ccgccccaat ggaccattgt cccgcgttcc ccgcgataca cggccccgta accgggacgg
     2041 acaattttac cgcctggctc ttgacccgtc cgttcttccg gcggtacagg ttcgcccggc
     2101 tgattccacg taagaatcca atcgggattc ttaaacgtgt aggtcacgtt catttccaca
     2161 cacgaatccc aaagaccttc ttcaggtggc gtgccggtag cctccacttc cacaggtcct
     2221 gttccgtcag cgcccatcca ccacatggca caactcataa catgggcacc gcggtcccgg
     2281 atttgtcccc cgcctgactc caaaagccac cgaaacacgc cgtgcaaata ccgcttgttg
     2341 aagggtcgcc aacgcagggg accaagccag aggtcccagt caagttccgg gggtgggtca
     2401 ctatccggag tgtggcttgt atcaactggg ctgggatagt gccagcagtc aacacgatat
     2461 acgcgaccaa tgacgccgtt caccagatac cgatgggcga gataggcctc cggttgagac
     2521 cgcccctggg agccaatctg gctggcgatc ttggctttgc gagccgccgc caccatggcc
     2581 ttgccctctt caatcgtgca gcaggctggc ttttcgcagt agatatgctt accacattca
     2641 gccgcgtgga cgaattggac cgcgtgccag tgatccggtg ttgcaatcac caccgcatcg
     2701 acatcttttc gttccaaaat gtaacggtaa tcgcgataga cgtccgcctg cggaccaaca
     2761 aggcgagaag cctcctcgag acgtttctcg tcaatgtcac aaacggccac gtggtcgcac
     2821 agccctgccg cacgcagacc cgccatctct cggtagagac cttttccccg cccacccatg
     2881 ccaatatggg caaccgtgat cctttcgttg gcgccgggct gcccttccca cgccatcgca
     2941 cgacggggga tgatctccgg cactccgact cctgaggccg ttgccgccaa gacactcgtt
     3001 tgcaaaaacc gacgtcggcc cagtcgctgg ctacgcgggc cgtcaacatt tcgttgaccc
     3061 attacgattc ctccacgaag tgatgccatt actgcgttca ataccggacc ggtaactccc
     3121 ccaggccggc cgatacgccg tcaccaaccg tttgtgtcat agccagaata acccgcagcg
     3181 cacgaggata caagcttttc acagattctg aggatttccc ccgcggaatt gcctcagccc
     3241 gcgaccaccg gcggaacgcg cggaaatccg cggatcagcc tggcgtcgcc ccgcggctaa
     3301 acgggtagcc tcacaccaaa aaatggcaat ctggacaata aaagagcaaa ttaaaaaatg
     3361 ccacaataga cgtaaatgca tactgtgtgt ccaaggaaga aacttcttcg agcgagaatc
     3421 gtttggcgtg gtgtttgcta caagatgacg gccgaagttc ccgcctgttt ttcaaccctc
     3481 cgcaacgacc caatcaacgc gaggagtgat tacaatgatt tgtatccgga ggcttcgcaa
     3541 gagcaaacag ccgtcttccc gtcggcaccg aatcaaagcg aagttcgcta ggagagtctt
     3601 ttcgatggat acagccccgc gtctggaatg gttaaatcac ggcgctccgc gggccgaatg
     3661 gtccgacgag gacctcgttc gcgagtacca gcagacgggg gaaccccgcg catttgaaga
     3721 gcttgtccgc cgttatgaac gggaattgta caactatctg tgtcaatatt taggcgatgc
     3781 ggaaatggct gaagacgcct tccaggccac cttccttcag gtccaccgga agtgctctca
     3841 gtttgattcg ggacggcgtt ttcgcccctg gttgtatgcc gtggctacga accaggccat
     3901 cgacgcccaa cgtcggaacc ggcgacatcg cctggtcagt ctcgatcagc aatcacggat
     3961 gtacacagaa agccacgatc tggggtgttt gattgacctt gtcgaggggc aggagatgga
     4021 tcccgaggaa catgttgaac tcaacgagcg taaagaagat gtccgccaag cggtggacca
     4081 gttaccggaa agccttcggc aggtccttct tttggtgtac taccaaggaa tgaagtaccg
     4141 cgaggccgcc gaggttttga atatccctgt gggaaccgtc aagagccgtt tgcacacggc
     4201 aatacgccga ctggaatatc acttgaagct tccgcggtca ccgagccatg cctgattgct
     4261 caccagccca actcattggg tacctcttga acgctctgga tgaagaagag cgggaggaaa
     4321 ttgagcaaga actggtgcga tgcccggagc tacgcaaaaa acttcagcga ttgaagcggc
     4381 gattggctgt cctcgagctt gggcgaaggg agattgcccc cccgccggga ttagctgacc
     4441 gaacttgcca gtttgttttt caaagctgtg ggtgtttagc catacgacat acaacgaagg
     4501 cgacaagccc cgccttgccg gccagtaggg atgttgcggg cttccggctt agccctctca
     4561 gcgactatct cgggggtcat ggggctaatt ggtcgttcgc cgaccttgtg gtgagcgcgt
     4621 tggttgtcct aatcgtggtg agcctcctt
//
LOCUS       DAOPTR010000158         4645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_14971_length_4645_cov_3.520261, whole genome shotgun sequence.
ACCESSION   DAOPTR010000158 DAOPTR010000000
VERSION     DAOPTR010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14971_length_4645_cov_3.520261"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="PKI05_04320"
     CDS             <1..659
                     /locus_tag="PKI05_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51456.1"
                     /translation="PGLRVREVDTGVEMQAGQTLAIAGLVETRVEAQRRGVPVISDLP
                     YVGSAFRRVAERSNDVELLILVTPELVEAMNAEEVPPCGPGTETASPTDWELYMKGYI
                     EVPRCCDPSVTNGAVNPAAGQPTPAPGVSAESAQTGNAASVRVARSNDGSGVPGLQAA
                     SPYAVSEGSSTTTFGKPGVVPQTRSARFVNDSSSPKPQPRPSEPSPLPGFKGEIGYDN
                     "
     gene            935..2128
                     /locus_tag="PKI05_04325"
     CDS             935..2128
                     /locus_tag="PKI05_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOA51457.1"
                     /translation="MSHVLRVAIVDPHDTGREELKRLLLGLDSIWLEAECSRYEFFAD
                     VVAQTNPEIGIVALDFDQEKALDLVSKVTQQSPDCAIFVVSSSHDGTVILKAMRAGAK
                     EFLPQPVRIDDLLAAIERLTELRLGRGDQKARGSTVIAMTGVTGGVGVSTVAVNLGCA
                     LARNDKNSVVLVDLDLCLGDADVLLDAIPDYTIVDVAQNVTRLDFSLLKRSLTKHSSG
                     LFLLPRPIQLEDVALVSAEDIQRVVGLLKASFTHLVMDLSKAFTPLDMMAMEMAQHIV
                     LVTQLDLPCLRNVVRLLMSFNNIQGMAEKVKIVVNRTGLESGQINIKKAEEIIGREIF
                     WQIPNDYRACSESRNNGVPLLESAPRSPVTHAIVGLAEALCGESAKTAEKKSAKGGLF
                     KLFAK"
     gene            complement(2149..3123)
                     /locus_tag="PKI05_04330"
     CDS             complement(2149..3123)
                     /locus_tag="PKI05_04330"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOA51458.1"
                     /translation="MLRPIDGSNSDFKSRSDQTHIGHLGFTLVELLVVIAIIGLLIGL
                     LLPAVQAAREAARRAECSNNLKQIGLAIQMYYDTNRRLPAGWTAYTADGRQPQVNGQP
                     GWAWGAVILPYLEQMNLYQGGIVLKAPIYDPVNEQAIQTFLTIYRCPSDLSQDRVFEA
                     HKLHEGHDHDQGHAGDEYDELLLARSNYVGSFGTTDIHVCEDTPPGGSCKGDGVLFHN
                     SYLDYSAISDGLSQTFLVGERTVALGYATWTGVLPGDACAPAAVLASAEFPPNLRKAH
                     AHNFSSQHPQGANFLLCDGSVHMISQTIDLNVFRALATRSGNETPGPF"
     gene            complement(3350..3802)
                     /locus_tag="PKI05_04335"
     CDS             complement(3350..3802)
                     /locus_tag="PKI05_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51459.1"
                     /translation="MPRRRVNIPLILVTTVWYALTAVMGASLHHHLGWHPVVGTSIRC
                     GGCYGSCGFKDSEGSYLLYSTAFAKDCDTANIGPHLRMVATFRSTVGAHQWSQCPICQ
                     FQVQPRTIQNIFQYVAGTYSHWAPQPSGVALVVFTPDFSWHSRAPPTV"
     gene            complement(3983..>4645)
                     /locus_tag="PKI05_04340"
     CDS             complement(3983..>4645)
                     /locus_tag="PKI05_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOA51460.1"
                     /translation="FINEIAAICERTGADINDVRRGIGHDSRIGFAFLFPGVGYGGSC
                     FPKDVRALAAVARAHDVVPRILEATHAVNEHQKGILLRKIREHFGGALSDKTIAVWGL
                     AFKPGTDDIREAPSLVVLDGLLKHGATIRVYDPEAMENVRRIYGNRLIYCAQRDDALN
                     DADALVILTEWKQFVHPDFEVMKSRLRRPVVFDGRNIYNPYRMRDLGFTYYSIGRPPV
                     RP"
BASE COUNT         1028 a         1288 c         1344 g          985 t
ORIGIN      
        1 ttcccggcct gcgtgttcga gaggttgaca ccggagtcga gatgcaggcg ggtcagacgc
       61 tggcgatcgc cgggttggtg gagacgcggg tggaagccca acgccgcggc gtgcccgtca
      121 tctccgatct tccctacgtt ggctctgctt ttcggcgtgt ggccgaacgc agcaacgacg
      181 tcgagttgct gattctggtc acgcccgagc tcgttgaagc catgaacgcc gaggaagtgc
      241 ccccgtgtgg accgggaacg gaaacggcta gtcccacgga ttgggaactc tatatgaaag
      301 gttatattga ggtcccacgc tgctgtgatc cttcggtgac caacggggcg gttaaccccg
      361 ctgcaggaca gcccaccccg gcacccggtg tttcggcgga gtcggcacag acgggaaacg
      421 ctgcgtcagt ccgggtggca cgatcgaacg atggcagcgg agtcccggga ttgcaagcag
      481 cttcaccgta tgcggtctcg gagggatcat ccacgaccac gtttggcaaa ccaggggttg
      541 tgccacagac tcgctcggcc cggtttgtca acgactcttc ctcgccaaag ccgcagccac
      601 gaccgtcgga gccatctccg ctgcccggat ttaagggcga aatcggttat gacaattgaa
      661 cgtaaaagag gggtggccac ggaatgatgg tccgtgcggg tttaagcgag aggatccttc
      721 gagcggacgg tgctcgcccg tgaagtcggc atctcgatag ttcttcactt ctttttgccc
      781 aacctaatta gcaagaatag ttttcctcac cccggaaaat aagccatatt tttgatggtg
      841 aggcgtcatg tgataaacaa gttctcctca gcccgatctt ccgcgggcac acgctgggga
      901 aacgtgtggt ttctaactaa ggtatcgggt gaatatgagt catgtgctgc gtgttgctat
      961 cgtcgatcct catgataccg gccgcgagga actgaaacgt ctcctgttgg gattagacag
     1021 tatctggctg gaggccgaat gctcgcgtta cgaattcttt gctgatgtcg tagcccagac
     1081 aaatcccgag atcggcatcg tggccttgga ttttgaccaa gaaaaggccc tcgacctcgt
     1141 ctccaaggtc acgcagcagt cgcctgattg tgcgattttt gtggtcagtt ctagccacga
     1201 tgggaccgtg attttgaagg caatgcgggc aggggcgaag gagtttcttc cgcagccggt
     1261 gcggattgat gatcttttgg cagcgatcga gcgtctcacc gagctgcggc ttgggcgggg
     1321 ggatcaaaag gcccggggat ctaccgtcat cgcgatgacc ggcgtgacgg gcggtgttgg
     1381 agtctcgacg gttgccgtca atcttggttg tgctctagcg cggaatgaca agaactcagt
     1441 cgtcctagtg gatttggact tgtgcctggg cgatgccgac gtcttgctcg acgccattcc
     1501 agactacacc atcgtcgatg ttgcccaaaa tgtgacccgg ctagatttct ccttgctcaa
     1561 acgttccttg accaaacaca gttcgggcct attcctgttg cctcgcccga ttcagttgga
     1621 agatgtcgct ttggtgagcg cagaggatat ccagcgggtg gttggcttgc tcaaggctag
     1681 tttcacccat cttgtcatgg acctttccaa agccttcaca cccctcgaca tgatggccat
     1741 ggagatggcc cagcacattg ttctcgtcac acagttggat cttccctgct tgcgtaatgt
     1801 ggtccggttg ctaatgtctt tcaacaacat ccaggggatg gcggaaaagg tcaagatcgt
     1861 ggttaaccgc accggcctgg agagcggtca aatcaacatc aagaaggccg aagagatcat
     1921 tggccgggag atcttctggc agattccgaa cgactaccgc gcatgtagcg aatcacgcaa
     1981 taacggagtg ccgctcttag agtcggcacc ccgttctcct gtgacccacg ccatcgttgg
     2041 actggcggag gccctttgcg gcgaatcggc caagaccgcc gagaagaagt cggccaaggg
     2101 cggcctcttc aaactctttg ccaagtgatg gcaacacccc aggtaggctc aaaacggtcc
     2161 tggtgtctcg ttgcccgatc gggtggctaa ggcccgaaac acgttgaggt cgatggtctg
     2221 actgatcatg tggaccgagc cgtcacagag caggaaatta gcgccctgtg gatgctggct
     2281 gctaaagttg tgggcatgtg ccttccgcag gttcggggga aattcagcag aggccagcac
     2341 ggcagcgggt gcacacgcat caccgggaag aacgcccgtc caagtggcat agcccaaggc
     2401 caccgtgcgc tccccaacca aaaacgtttg actaagaccg tccgagatgg ccgaataatc
     2461 caagtaactg ttgtggaaaa gaactccatc tcccttgcat gacccgccag gtggcgtatc
     2521 ttcgcagacg tggatatcgg ttgtcccaaa tgacccaaca tagttggaac gcgccaaaag
     2581 gagttcgtca tactcatcgc ccgcgtgccc ttggtcatga tcgtggccct cgtgcagctt
     2641 atgggcctcg aagactcgat cctgactcaa atcggatggg cagcggtaga tggtcaaaaa
     2701 ggtttggatg gcctgctcgt tgacggggtc gtaaatgggc gctttaagca cgatgccccc
     2761 ttgatagagg ttcatctgct ccaagtaggg caagataaca gccccccagg cccaacccgg
     2821 ttgaccattg acttggggct gccgcccgtc ggcggtgtag gcggtccatc ccgcgggcaa
     2881 tctccggttg gtgtcgtaat acatttgaat cgccaagccg atttgcttga ggttgttaga
     2941 gcactcggcc cgcctagccg cttctcgggc agcttggacg gctggcaaca gtaatccgat
     3001 cagcaggccg atgatcgcaa tcaccaccaa caactcgaca agtgtgaaac caaggtggcc
     3061 aatgtgtgtt tggtcactcc gggatttaaa atcgctattt gatccgtcga tcggtctgag
     3121 catggaaaac ctcctcaacc gtgcgaaaaa actgcgtcga agaaatcgcc gaaaagaaaa
     3181 cgtctcaatg ctcacgcatc atccagcatg cgcgtcattg ctggttggca acactgccac
     3241 gcacacatgt tgttctgggg catgcgttac caggcccgta atcaacgcca attgaccggt
     3301 cgggccacgg cttcgtcgct ggagtcagcg aactttgaca aggcaccatt cacacggtgg
     3361 gaggggcacg actgtgccag gaaaaatccg gtgtaaaaac aaccaacgcc acaccggagg
     3421 gctgaggcgc ccaatggctg taggtacccg cgacgtattg gaagatattc tgaatcgtcc
     3481 gcggttggac ctgaaactga cagatggggc attgcgacca ttgatgggcg ccaacggtcg
     3541 agcggaaggt ggccaccatc cgcagatgag ggccgatatt tgccgtatca caatctttgg
     3601 caaaggccgt cgagtagagg agataactgc cctcggaatc cttaaatcca caagaaccgt
     3661 aacagccgcc acaacggatc gacgtaccga ccaccgggtg ccagcccagg tgatggtgta
     3721 acgacgcacc catcacggct gtcagggcgt accacaccgt ggtaacgagg atcagcggta
     3781 tgtttacccg tctgcgaggc atggggcgaa aacaagccgt ttcatgttga ccaaccttta
     3841 ggccccatta tatcaagtag ggcacatccc ggccaggatt gtgcaatcac tgcatttgat
     3901 ggtttcacct ccggcttacg ccaaaggctc aagaagtttc accggcagcc tacgcccgag
     3961 gcgcggatat tctacacaag tgtcatgggc gcacaggcgg acggccgatg ctgtaatagg
     4021 tgaatcctaa atccctcatg cgatagggat tgtagatatt ccgcccatca aacacgacgg
     4081 ggcgacggag gcgggatttc atgacctcga aatcgggatg tacgaactgc ttccactccg
     4141 tcagaatcac cagggcatcg gcgtcgttga gggcatcgtc tcgttgcgca caatagatta
     4201 gacggttgcc atagatgcga cgcacgttct ccatggcctc tgggtcgtaa acgcggatgg
     4261 ttgccccatg cttcaaaagc ccgtcgagaa cgacaagcga cggggcctca cggatgtcgt
     4321 cagttccagg cttaaaggcc aatccccaca cggcgatcgt tttgtcggac agggcacccc
     4381 caaagtgctc gcggattttc cgcagcaaga tacctttttg gtgctcgtta acggcatgcg
     4441 tggcctccag aattcggggc accacatcat gcgctcttgc cactgcggcc agggcgcgta
     4501 catccttggg aaagcaactt cccccgtaac caacgccggg gaagaggaat gcaaacccaa
     4561 tgcggctatc atgaccaatg ccccgccgga cgtcgttgat atcggcccct gtccgttcgc
     4621 agatagccgc gatctcgttg atgaa
//
LOCUS       DAOPTR010000159         4632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_15030_length_4632_cov_3.379288, whole genome shotgun sequence.
ACCESSION   DAOPTR010000159 DAOPTR010000000
VERSION     DAOPTR010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15030_length_4632_cov_3.379288"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="PKI05_04345"
     CDS             <1..1249
                     /locus_tag="PKI05_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51461.1"
                     /translation="LKATQEDHQAMDLDRTSSRRWPTARYLPLSITPGPGGSAGERTS
                     NYVAGDYAGAWRIVFAKDTPPEQGMAKVRSALITLQRDRAAAVADRVTRLAELKRQLQ
                     DLQTRFPQEMETLRHEWRERQTEFSRLAMSAIQNQVVRGQLANDPLLAQLQATRESLL
                     KRRQQLLMTHTVEHPDVKAVESLLAEVEQEIQLRSRRQKEYSANGVPGETNVPAATTW
                     LSLPLGGQIGAPSAYEAPAIGNSHERPGLASNNQGEMQRLAALQSEIQSWDAKLGKGL
                     DELRGNLEELQGQFVAVEAQLRDALTDWEVAQGCVVDFDRESLSSVPREVTKTLTPFS
                     LLFALLVGLLAHGAVTSLIIGFADGQRVSTAEQLFAITGGPVFHINLSTGDGKFIDGM
                     DSVSPPHFATTQVESELSSKPRV"
     gene            1268..3100
                     /locus_tag="PKI05_04350"
     CDS             1268..3100
                     /locus_tag="PKI05_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOA51462.1"
                     /translation="MYEQFFHFKERPFLPAPYPRAYVPVETMESAREQLRRAILRGEG
                     PALLVGPPGTGKTLLLQVLAEECGSRFRVVFLRHGRFPTSRALLQTILHELKQPFQGM
                     EDGELRIALMDFLKDEGGPAVVILADEADTLKLRLLEELRVLTNIQRSREPGLRLVLA
                     GSPVLEERLTSPKLQTLNQRIAVRCYLDALNRADTFTYIRTQISRAGAKAEQIFPENA
                     LQAVYHATGGVPRLINQICDHALVLAFADGLRQITAEVVEQAWADLQQIPLPWNPTRP
                     STPNTKGVIEFGSLQDETPDRDLFSVDQGNNGVSEGTSRFGFLEKPELPSNVAPHLDD
                     TAISSSSKSETEDEGHEIEAVSVENVMAVPNAGTSSSGEPALGDRPDPVTQIEQIEQA
                     IQSLQEEAPPSAVKRPQAELIFYDWGDPFEETFMRQIPFICGSGAEGYTDFEDDVATD
                     EGPTNCVMPSPQNPTDEHDPPNSEFDRAESAQTNLVGQGGEISGSGDPLSQKQTSTSL
                     TVTGSAPTEELRSDSSEGDEFPTDGNDHSDPETPPPSPIGKLTHPMPEPDPTGEPEAG
                     TPVTLRFPMNIVHMEGGADKPQKYRAIMKSLFTRLGKSGLEPIP"
     gene            3296..4186
                     /locus_tag="PKI05_04355"
     CDS             3296..4186
                     /locus_tag="PKI05_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51463.1"
                     /translation="MSRILEAIQRLEEDERAEDRSVIPIFEGLAKNVAGSAPSPTGES
                     SPSDVSESDVRVFVRRFDSSHGETNNLEPPHFGKDDVQAATRATDDELEQRVMAICTP
                     YLKTIPAALKETWVLSAMWKTAATHAGWISSQVLDPHVPIECALALSSDVCEKIGQSV
                     LLIDGDPSSRLTRTLGLSPECDLIDVMLGRATLEESLIRLTRGGLYCLPIRQVGSAWV
                     LPNWADLNGLFQAVRQAFGFAVAFCPMSDDPLSLRILRNSDEFWLWCAAGSTPAGQIK
                     KVIQAASPKGDPAGAVLISL"
     gene            complement(4261..>4632)
                     /locus_tag="PKI05_04360"
     CDS             complement(4261..>4632)
                     /locus_tag="PKI05_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylgalactosamine-6-sulfatase"
                     /protein_id="HOA51464.1"
                     /translation="FGDLMATFCELTGADLPPGRDSISFLPTLLGKSEEQKQHKYLYW
                     EFYERGSAQAVRFGKWKAVRRPMLTGEIELYDLEADIGETTNIAAQHPDIVAQARQFM
                     EEAHVNDPRWQVPKPVVKPAG"
BASE COUNT         1024 a         1264 c         1338 g         1006 t
ORIGIN      
        1 tttgaaagcg actcaggaag accaccaggc aatggactta gatagaacgt cttcacggcg
       61 ttggcccacc gcccggtatc tgccgctgag catcacacct ggaccaggtg gctccgctgg
      121 cgaacgaacc agcaactatg tcgcagggga ttacgcgggg gcatggagaa tcgtatttgc
      181 gaaggacacg cctccggagc agggcatggc gaaagtccgt tctgctctta tcacactgca
      241 aagagaccga gccgccgccg ttgccgatcg tgttacacgg ctggcagagc ttaaacgtca
      301 attgcaggac ttgcaaacac ggtttccaca ggaaatggaa acgttacgtc acgagtggcg
      361 agaaaggcag acggagtttt ctcggctggc gatgtcggcg attcaaaacc aggtcgtgcg
      421 cggtcagctg gccaacgatc ccctgctcgc ccaattgcaa gcaacacgtg agagccttct
      481 caagcgtcgt caacagctac tgatgaccca caccgtcgag catccggacg ttaaagcggt
      541 ggaatcgctt ctggccgagg ttgaacaaga gatccaatta cgatctcggc gacagaagga
      601 atactcggca aacggtgttc cgggagaaac aaacgtcccc gcggccacga cctggctctc
      661 cctcccgctt ggtggacaaa ttggtgctcc gtctgcctat gaggcgcccg cgatagggaa
      721 ctcacatgag cggcctggat tagcgtcaaa caaccagggg gagatgcagc gtcttgcggc
      781 tctgcaatcg gagattcaat cgtgggacgc aaagctggga aagggccttg acgagcttcg
      841 cggcaatctt gaggagttgc agggacagtt cgtcgcagtt gaggctcaac tccgagacgc
      901 cttgaccgac tgggaggttg cccaaggatg cgtcgtcgat ttcgacagag aatccctatc
      961 ctcggtcccc agggaagtga caaagactct gacgccgttt tcgctgttgt ttgccctttt
     1021 ggtgggacta ttggcgcatg gggccgtcac gtcactaata attggtttcg ccgacggcca
     1081 gcgtgtttct actgctgagc aattatttgc catcacgggg ggaccggttt ttcacataaa
     1141 cctgtccacc ggggatggga aatttatcga tggcatggat tcggtatctc caccccattt
     1201 tgcgacgaca caggtggagt cagaactgag ctcaaaaccg agagtctaaa tctacacgga
     1261 tgggtcgatg tacgagcagt tttttcattt caaggagaga ccgttcttgc cggcacctta
     1321 cccgcgggct tacgtgcccg tggaaacaat ggaatctgcc cgtgagcaac ttcgccgagc
     1381 gattttgcgg ggtgaaggtc cggcgctctt agttggtcca ccgggcacag gcaagacatt
     1441 attactgcag gtcctggcgg aggaatgcgg ttcccgtttt cgtgtggtct ttttgcgtca
     1501 tgggcggttt cccacttccc gtgccctcct ccagaccatt cttcacgaac tgaaacagcc
     1561 ctttcagggc atggaagatg gcgagttgcg gattgccctt atggacttcc ttaaagatga
     1621 aggtggccca gcggtggtga tcctggcaga tgaggcagac accctcaagc tccgcctttt
     1681 ggaagaactg cgcgttctga caaacatcca gcggagccgt gaaccagggc tgcggctggt
     1741 tctcgccggc tcaccggtct tggaagaaag gttgacctcg ccgaaactac agacgttgaa
     1801 ccaacgaatt gccgtccgct gttatctgga cgccctcaac cgggccgaca cctttaccta
     1861 cattcgcact caaatcagtc gggctggtgc taaagccgag caaatctttc cggaaaacgc
     1921 tttgcaagcg gtgtatcacg ccacgggcgg cgtgccgcga ttgatcaacc aaatttgtga
     1981 tcacgccttg gtcctggcat ttgccgatgg ccttcggcag attaccgcgg aggtcgttga
     2041 gcaggcatgg gcagacttac agcagatccc tctgccctgg aatcccacac gaccttcgac
     2101 accaaacacc aaaggggtca tcgaattcgg ttcattacag gatgaaacgc cagatcgaga
     2161 cctgttttcg gtcgatcagg gaaacaacgg tgtgtcggaa ggcacctccc gctttggatt
     2221 tttggaaaag ccagagttgc cgtccaacgt ggcaccacac ctcgacgata cggccatttc
     2281 gtcaagctct aaaagtgaga cggaagatga aggccacgag attgaagcgg tatcggtcga
     2341 aaacgttatg gcagtcccaa atgcggggac cagctcatca ggggagcctg cattaggaga
     2401 tcggcctgat ccggtgaccc agattgagca gattgagcag gccatccaat cgttgcagga
     2461 ggaagctccg ccttctgcgg tcaaacggcc gcaagccgag ctcatcttct atgattgggg
     2521 tgatcctttt gaagagacgt tcatgcggca aattcctttt atctgtggca gtggtgctga
     2581 gggctatacc gatttcgaag atgacgttgc caccgacgag ggacccacaa actgcgtgat
     2641 gccgtcaccg caaaacccca ccgacgagca tgatcctcct aattctgaat ttgatcgggc
     2701 ggaatctgcc cagacgaact tagtcgggca aggaggagaa atttcaggta gcggtgaccc
     2761 gctttcacaa aagcaaacat cgacttcact caccgtcacc ggctctgcac cgactgaaga
     2821 gctgcggagt gatagctctg agggggatga atttccgacg gatgggaacg accattccga
     2881 tcccgaaacg ccacctccct cgccaattgg gaagctgacg catccaatgc cggaacccga
     2941 tccgacaggt gaaccagaag cggggacacc agtgacgctg cgttttccga tgaatatcgt
     3001 gcacatggag ggcggtgccg ataagcccca gaagtaccgc gcaatcatga agtcattatt
     3061 tacgcggctg ggaaagagcg gcttagagcc tatcccataa cctcccctcg cccatttggc
     3121 gggcgagggg tcgggggtga gggtgactcg aatcgctgcc ggttttctca atcgattctg
     3181 atgttcgctt gccctcaccc caaccctccc ccggagggag agggggactt tttgggatag
     3241 gctctaactg gcagtgcccg tgttggcgtg aatgaattag gagccaatca aaaagatgag
     3301 tcgaattttg gaagcgatcc agcgtctcga ggaggacgaa agagcggaag acaggtccgt
     3361 catcccgatc tttgagggcc tggcaaaaaa cgtggcaggc tccgcgccgt cccctaccgg
     3421 tgagtcttct ccatccgatg tgtcggaatc cgacgtgcgc gtgtttgtac gtcgttttga
     3481 ctcaagccac ggcgagacca ataatcttga gccgccgcat tttggtaaag acgacgttca
     3541 ggctgcgacg cgggcaactg atgacgagct tgagcagcgc gtcatggcta tttgtacacc
     3601 atatttaaag acgatccctg ccgcgcttaa agagacgtgg gtgctttcgg ccatgtggaa
     3661 gaccgctgcc acccatgccg gctggatcag tagtcaagtt ctcgatccgc atgtccccat
     3721 cgagtgcgca ttggcgcttt cgtcggatgt ttgcgagaag atcggccagt ccgtgctcct
     3781 tatcgacggc gacccctcct cgcggttaac gcgaactctt ggattgtcgc ccgagtgcga
     3841 tttgatcgac gtgatgcttg gccgagccac tttggaggaa agcctcatcc gactcacccg
     3901 cggtgggctt tactgcctac ctatccggca ggtgggatca gcctgggtgt tgcccaactg
     3961 ggcggatttg aatggcctct tccaggcggt gcgtcaggct tttggattcg ctgttgcgtt
     4021 ctgccctatg agcgatgatc cattgtcatt gcggattctt cgtaatagcg acgaattctg
     4081 gctgtggtgt gcagcgggat ccacgccggc aggtcaaatc aaaaaggtca tccaagccgc
     4141 gagtccaaaa ggcgatcccg ccggggcggt cttaatttcg ctgtgaaggc cacgcagcac
     4201 gctttcccta tcggtttggg agaacggttt aaagcgtttc ggctggaacg cagctcggta
     4261 ttatccggcg ggcttaacga cgggcttcgg cacctgccag cgaggatcgt tcacatgggc
     4321 ctcttccatg aactgacgtg cttgggcgac gatatcggga tgctgggcag cgatgttggt
     4381 cgtttccccg atgtcggcct ccaagtcgta gagttcaatc tcacccgtca gcatgggtcg
     4441 gcggacggcc ttccattttc caaagcgcac ggcctgtgcc gacccccgtt cgtaaaactc
     4501 ccaatataga tacttgtgtt gcttctgttc ctcggatttg cctaacaaag tgggcagaaa
     4561 gctaatggaa tctcgcccgg ggggaaggtc ggccccggtg agttcacaga aggtggccat
     4621 cagatcgcca aa
//
LOCUS       DAOPTR010000160         4623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_15058_length_4623_cov_3.201839, whole genome shotgun sequence.
ACCESSION   DAOPTR010000160 DAOPTR010000000
VERSION     DAOPTR010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15058_length_4623_cov_3.201839"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..291)
                     /locus_tag="PKI05_04365"
     tRNA            complement(219..291)
                     /locus_tag="PKI05_04365"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(256..258),aa:Arg,seq:tct)
     gene            947..1987
                     /locus_tag="PKI05_04370"
     CDS             947..1987
                     /locus_tag="PKI05_04370"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOA51465.1"
                     /translation="MAEEGKVLVTGATGFIGTRLVQALVERGRSVRALCRRDNVTSPP
                     GLGLRDGAPLRHPKVELFRGDVTDAESVNRAMEGCTEVFHVAGYAKNWAPDPTTFYRV
                     NRDGVKNVLAAAQRCGVRRVVWTSTIQTFPPTPPGQIGDETWVNRGPFLTDYEASKVA
                     AEKEALAMARQGLDVVIVNPTRVFGPGHITEGNSLSRLIDMYDRGRLPFLLNAGRNIG
                     NYVFVEDVVEGHLLAMERGRSGEKYILGGENVSLREFFRLVDQVSDKRHFQIPIFRPG
                     ALTFAWVQKKLAEWFGIYPAITPGWVRTFLMDRAYTCRKAQEELGYQPRPLIEGLRIT
                     YAWLQRVREEQD"
     gene            2005..2706
                     /locus_tag="PKI05_04375"
     CDS             2005..2706
                     /locus_tag="PKI05_04375"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOA51466.1"
                     /translation="MPFWSALEERQKTAAVTLFLSTVVMITWRYFGSAEFYREHLADR
                     LALGLDPRAAAAWYRFVTGFALLGLMPALIGGHLLGRGLASYGVRLGILVRTVRSAAL
                     LAPIFIFLGWISSHDPSMARCFPTNPAAGSSLAMFALHAAIYLLHYAGWEFHFRGFLQ
                     MGLRDSLGDGNALLVQVMASTLVHIGSPAIETYGAIAGGILWGILAYRTQSLLSGMVQ
                     HACLGIALDAFLCFR"
     gene            3093..4061
                     /locus_tag="PKI05_04380"
     CDS             3093..4061
                     /locus_tag="PKI05_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOA51467.1"
                     /translation="MGPSVCASEVLYEKGDDMRMGFTLVELLVVIAIIGLLVAILLPA
                     VQAAREAARRAQCANHIKQITLGMHEYHDTVGAFPFGFSTLEQSWHAMLLPYVEQEAL
                     YKTLIFQETGPGNWDSGSANTRACQTVIPVFRCPSLPIPTHVDHDIPQRVPSSYNGIA
                     SSQATSDDLSSIPQGYPRVALEQIDLDGMLFGNSSIRFAEVVDGTSHTLLVGEMHTDP
                     DFKKDGQSMDYWHIGLPHTGNWVPGGTGGTEYSEAIASTYPPINGWKDSELPGVVMEL
                     SCGSYHPGGAMFGFVDGSVRFIQETIDISVYRALGSRNGQESITGF"
     gene            4398..>4623
                     /locus_tag="PKI05_04385"
     CDS             4398..>4623
                     /locus_tag="PKI05_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51468.1"
                     /translation="MGRMKVYSGTVSVTLLLGLAVGIWAAIGLIGGPIGSLHQALADE
                     AVGPVVMEEGQAETQEAEAAKEEKPAEEAKP"
BASE COUNT          932 a         1177 c         1374 g         1140 t
ORIGIN      
        1 cttcggcgat gagcacagtt tagcgctttt gcgatcagca tagtttgccg cttttgcgat
       61 cagcacagtt tgaaaaacac ccccgcaaaa cgcaacgcaa aatgcaacgc agaacctgca
      121 agaaatgcat tgcaagacga tgaagtgcaa ttgtgataga acaaatagac actatttgta
      181 aatcaagacg ctgcaacgag ttgcgacatt atacaaagca cgcccggagg gattcgaacc
      241 cccaaccagc ggattagaaa tccgctgctc tatcccttga gctacgggcg cgagtgatag
      301 tcgatatctt tcctacactc acctaggcaa tgcctttttg gttatccagc ttgccaatgt
      361 tcccagtatt gccagattct ttttgctttc aaattgttaa tcccacttgt tttcgcagcc
      421 aacccctaaa gcctgagttg ccagattgtc tggaagctta agcgtcgggt ttcccccaat
      481 tacgggcgac ggcgaacatc ggcccgaatc ggtgcttcag ggaccacagc ggaactatcg
      541 ccgttctgca ctgggccgtt tgttactgat gtgtggatgg tactgagttt tcgcccacgt
      601 ccccccattt ggcggcggtt ctacccggtt cacctcattt cacattatcg gccagcatga
      661 cacccggcaa gggttaccgt cacggctagg cagcctcttc ttcggggggg tccctagctt
      721 cgccaagttg cccaaccgtt caacccaccg agcacgtatt cggctcagtt tcactgtagc
      781 gtgcgatcgt tacctaaact tcccaaaact gctgtttgac ggttgggatg gggtggcata
      841 taatcgcccg cggcggcggg tggcaggttg ggccgaaggg tgcggcttgt agcttcgtga
      901 cgggagagcg gacacgcggc aaagctggta ggagcgatac tagagcatgg ccgaggaagg
      961 caaagtcttg gtgacgggtg cgaccggatt tattggcacc cgattggttc aggctttggt
     1021 cgagcgggga cgctcggtgc gtgctctgtg tcggcgtgac aacgtcacct ctccgccagg
     1081 tctggggttg cgtgatgggg caccgttacg gcatccgaag gtagagttat tccggggtga
     1141 tgtcaccgat gcagaatcag tcaaccgggc gatggagggc tgtaccgagg tcttccatgt
     1201 agcgggctac gccaaaaact gggcaccgga cccgaccacg ttctatcgag tgaatcgaga
     1261 cggagtcaaa aatgtattag cggcggccca acgttgcggg gtgcggcggg ttgtgtggac
     1321 ctcgacgatc cagaccttcc ccccgacgcc tccgggtcaa attggtgatg agacctgggt
     1381 aaaccgcggt cccttcctga ccgactatga ggccagtaag gttgccgctg agaaagaggc
     1441 cctcgctatg gcccgacaag gcctcgatgt ggtcattgtc aatccgacac gggtctttgg
     1501 tcccggccat atcactgagg gcaattcgct ctcacgactg atcgatatgt acgatcgtgg
     1561 gcgtttaccg tttcttctca acgccggtcg gaacattgga aattatgtgt ttgtggaaga
     1621 cgtggtcgag gggcatctgt tggccatgga gcggggtcgg agtggtgaaa agtatatcct
     1681 gggtggcgag aacgtgagcc tgcgagaatt cttccgattg gtggatcagg tcagcgacaa
     1741 gcggcatttt cagataccga tatttcggcc tggtgccctg acgttcgcgt gggttcaaaa
     1801 gaagcttgcg gaatggtttg gcatttatcc ggccattacc cctggctggg tccggacgtt
     1861 tttgatggat cgcgcataca catgccgtaa ggcccaagaa gagcttggtt atcagccgcg
     1921 acccttgatc gaaggattgc gcatcacgta tgcctggctc caacgagtgc gggaggaaca
     1981 agattgagag actggatgat gcgagtgccg ttttggtctg cccttgagga gcggcagaag
     2041 accgctgccg tcacgttgtt cctctccaca gtggtgatga tcacgtggag gtattttggc
     2101 tccgcggagt tctatcggga acatttggcc gatcgcttgg cgttgggact tgatccgcgg
     2161 gcggcggctg catggtatcg gtttgtcacg ggttttgcgt tgttgggtct tatgcccgcg
     2221 ttgatcggcg gtcacctctt ggggcgaggt ttggccagct atggcgtgcg actggggatt
     2281 cttgttcgca cagtgcgaag tgccgcgctg cttgctccca tcttcatttt tctcggctgg
     2341 atctcgtctc atgatccatc gatggcccgc tgttttccaa ccaaccccgc agcgggaagt
     2401 tctctggcca tgttcgcact ccatgcggcg atctatttac ttcactacgc aggttgggag
     2461 ttccacttcc gcgggtttct ccagatgggg ctgcgcgatt ctttgggtga tgggaacgcc
     2521 ctattagtcc aagtgatggc ctccacattg gtccatattg gttcaccggc cattgagaca
     2581 tatggtgcca tcgctggtgg cattttatgg ggcattttgg cgtatcgcac gcagtcgttg
     2641 ctgtcgggga tggtccagca cgcctgcctg ggtatcgcgt tggacgcatt cttgtgtttc
     2701 cgctaagggt ctatcccccc taatcttccc tctcccgttc ggcgggagag gggctcgagg
     2761 gtgagggtgc ctcgaatcgc tgtcggtttt gtcgatcgat tccgacgatc gtttgccctc
     2821 accccaaccc tctcccggag ggagagggga actttttggg atagactcga agcctgggac
     2881 ggtcctcaag ttcggcccgc agaaaccgtc ttctcctgag gtctttatgg atgggcagaa
     2941 tctgcgcgat gtcaaagagt aagcacgaaa ccccgtcgtt accagatcgt tgatacgctc
     3001 tatcccacta ggttgtcttc gctttgggct ccaaagagag tttgttgacg tttgccgccg
     3061 actggttgtc tgcaagaggt gggtttttta agatggggcc atccgtatgt gcaagtgagg
     3121 tgctttacga aaaaggggac gacatgagga tgggcttcac gttagtcgaa ttgctggtgg
     3181 tgatcgccat tattgggctt ctagttgcga tacttcttcc ggcggtgcaa gcggcacgtg
     3241 aagctgctcg tcgcgctcag tgtgccaatc atataaagca aatcaccctg gggatgcatg
     3301 aataccatga caccgtgggg gcgtttccct ttggttttag taccttggaa caatcctggc
     3361 atgccatgct tcttccgtat gtcgagcagg aggcccttta taagaccctt atctttcaag
     3421 agacgggacc agggaattgg gactctggct ccgccaatac gcgggcatgt caaaccgtga
     3481 tcccggtgtt ccggtgtccc agtttgccca tcccaaccca tgtggatcac gacatccccc
     3541 agcgcgttcc aagcagctac aacgggattg cttcgtcgca ggcaacctcg gacgatttga
     3601 gtagtattcc ccagggttat ccgcgggtgg cgttagagca gattgacctg gatgggatgc
     3661 tcttcggcaa tagctccatc cggtttgcgg aggtcgttga cggcacaagc cacaccttgc
     3721 ttgtcggcga gatgcacaca gatcccgatt tcaaaaagga tggccagtcc atggactact
     3781 ggcacatcgg cctacctcat acaggaaact gggtgcctgg tgggacaggc ggaaccgagt
     3841 actccgaggc cattgcttcg acctatccgc ccatcaatgg ctggaaggat tccgaactac
     3901 ccggcgtcgt catggaactt tcatgtggca gctaccatcc tggtggagcc atgttcgggt
     3961 ttgtcgatgg ctccgtgcga ttcatccagg aaacgatcga catctctgtt tatcgggcgt
     4021 tgggctctcg caacgggcag gagagcatca cgggctttta acgttcattt ccccatcttt
     4081 ttgtgcgcta tccgggaacc agtttgcggg ctaaggtgga gttgcttgga ggattctggt
     4141 tgtaatgatt tggaagtcta gacaagatat gctgacaagc gtaaataaaa cgactatgat
     4201 gagccaattg ccgtatgttg tttgagggga gtttcgccgc ccgcggggaa aacgatcccc
     4261 ccgacgcgct gttaccgggg catgacctat ggttaattgg ttacgtcttg tgtcattccg
     4321 ttagcagtca ggaattgcga agccttctga caaaggtatc gtggattgag tttttgcgca
     4381 tgagaggagc ttcttttatg ggccgcatga aggtttacag tggtacagtc agcgttaccc
     4441 tcttgcttgg gttggcggtc ggaatatggg ctgcgatagg actgatcggg ggaccaattg
     4501 ggagcctcca ccaggcgttg gccgacgagg cggttgggcc cgttgtgatg gaagaggggc
     4561 aagctgaaac ccaggaggca gaagcggcta aagaggagaa gcctgccgaa gaggctaaac
     4621 cca
//
LOCUS       DAOPTR010000161         4613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_15109_length_4613_cov_3.658622, whole genome shotgun sequence.
ACCESSION   DAOPTR010000161 DAOPTR010000000
VERSION     DAOPTR010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15109_length_4613_cov_3.658622"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="PKI05_04390"
     CDS             complement(<1..668)
                     /locus_tag="PKI05_04390"
                     /inference="COORDINATES: protein motif:HMM:NF040416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51469.1"
                     /translation="MSHQLWYTSAERGLAAGTRGFCTVKATHGIPRPLAQKLESLSGY
                     THLFPPHDKGNPANPVAYHFVCVYQGGKKWYVLSRVCDAGLDYSGRSNKFAHHVVLDQ
                     HELPPAGPAWLMGQPGFFDKTWDGVVGVVDSRKSIPQSDSPPRPCIQWETVTGDAGWA
                     GLLAESVLNEDGPPVHIIAPPNLSVLPLLEEAIALLPPELRWSATFSTYYTEQFPADV
                     TVRWR"
     gene            complement(782..2128)
                     /locus_tag="PKI05_04395"
     CDS             complement(782..2128)
                     /locus_tag="PKI05_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51470.1"
                     /translation="MSQAIQPQASVRGKIVCPHCWRDFAVYDVLYIASHPDLVGDPRL
                     GPEHPRRFLPTRFTFDCAALDAKGMPCRRTACPHCHLEIPRAILELKPRVVSVIGAPA
                     CGKSYFITAQSWALRTVLCSQFCVSFADADPLINQRLNDYESLLFLNPNEDDIVGLPK
                     TETFGDFYNSVLFGGQAVQYLKPFIFTLKPTANHPNARGATHLGRVLCLYDNAGESFL
                     PGQEHVMQPVTGHLAASDLFMFLFDPTQDVRIRRALSTVTSGDGDKTAAIRNRQATFR
                     QDSVLNEAAQRITRGYRFTAKSQRPPLIVVVTKVDVWRALLGEIDFSPPWRFIERVGL
                     AGVEIDTIEAVSQHVRSLLLQLTPEIVNTAEDLCDRVAYIPVSATGTAPVFDETRNVW
                     GFRPRSLKPIWTEVPLLYYFAKWERGIIGTTRTSRKSEQTEKTSELAAPAPSPTDE"
     gene            complement(2138..2788)
                     /locus_tag="PKI05_04400"
     CDS             complement(2138..2788)
                     /locus_tag="PKI05_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51471.1"
                     /translation="MYLFRESRLVVLILVAASLLIIVNGCRRPTGQPSNRSHVGDSSE
                     QGGGKDRQQADSNGPPGRSTREAPSSAESSHRDGRDSSRRPIGNDSSDVESDGSLGDR
                     GPGQPSGKTGAKSAPATSGEGVGHGSFGGESGRSSAARNSAVQKARDVLTQSNESDAR
                     RRYQSLLEAWQSLGPFTENDPEAAALANRLLQQMEKVGSTFDQRESPDVDKALIAQ"
     gene            complement(3237..4511)
                     /locus_tag="PKI05_04405"
     CDS             complement(3237..4511)
                     /locus_tag="PKI05_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA51472.1"
                     /translation="MSKSSEKLEVVIVGGGMITHDQILPSLYHLQRLGKVGSIKICAL
                     NSAPLVALARSERLLEAFPGHTFEPFPSLAEPPDRFFPDLFREVIGRLPARNLVVVAV
                     PDHLHDMVIREAIERDQHVLCVKPLVPTYRQAVAIEELARKRGLLVAVEYHKRFDRRS
                     LDARWQYRKGRFGEFRLGEARLIEPYYYRHSNFQNWFTKENSDPFTYVGCHYVDLVYF
                     ITGLRPVEVSVRGVEGRFPNGNIGWLWSAGTVIWENGAAMTLLNGLGYPDAGAGTNDQ
                     GLTMYCEGPDCGGILHHDDQYRGVSHGYTDTRSGAMFRYVSPDYMRLVPWEGEGLRPV
                     GYGYDSICAIVEAALRVNAAAAGLDGEAALAARQHVLREIDQRGILATPANSWINELV
                     TEAARMSIAADGRWMEIVYEPQPHVREKESLA"
BASE COUNT         1050 a         1343 c         1211 g         1009 t
ORIGIN      
        1 cgccaccgca cagtcacatc ggccgggaat tgctcggtgt agtaggtgga aaaggtcgcc
       61 gaccagcgaa gctcaggagg cagcaaggcg atcgcttcct ccaacaaagg aaggacagat
      121 aagttcggtg gcgcgataat gtggacaggc ggaccgtctt cattgagcac ggattcggct
      181 agaagacccg cccatccagc atcccccgtt accgtctccc actgaatgca aggccgcggc
      241 ggggagtcac tttgcgggat tgatttgcgg ctatcaacca cgccgactac cccgtcccag
      301 gttttgtcga agaaacccgg ttgacccatc aaccaggcgg gaccggccgg cggcaattca
      361 tgttgatcca aaacaacgtg gtgggcgaat ttattactgc gaccggaata atctagccca
      421 gcatcacaga cccggctcaa aacgtaccat ttctttccgc cttggtaaac acacacgaag
      481 tggtacgcca ctggattggc aggattccct ttatcgtgcg gcgggaataa atgagtataa
      541 ccgctcaagg actccagctt ctgcgccaac ggtctgggaa taccatgtgt tgccttgacg
      601 gtacaaaatc cgcgcgttcc tgctgccaaa cccctttccg cggaggtgta ccagagttga
      661 tggctcacgg gcgtctcctt ttaccttagc acctattcct gacgcgacac tcaccctctc
      721 ccgaattctc tttcgggctc gcgggttcac cccaaatgcc gagttttttt ggccgtaccg
      781 atcactcgtc cgtggggctg ggtgccggtg cggccaattc cgaagtcttt tccgtttgtt
      841 cagatttgcg gctcgtacgt gtcgttccga taatccctcg ttcccatttc gcgaagtagt
      901 atagcaaggg gacctcggtc caaattggct tcagcgatct tggacgaaat ccccagacgt
      961 ttcgtgtctc gtcgaagacc ggtgcggtcc ccgttgcgga aacgggaatg taagcgacgc
     1021 gatcgcaaag atcttcggcg gtattcacaa tttccggtgt caactggaga agcaaggacc
     1081 taacgtgctg tgacacggcc tcaatcgtgt caatttcaac gcccgccaaa ccgacccgct
     1141 cgatgaatcg ccacggcggg gaaaaatcga tttcgccgag caacgctcgc caaacgtcca
     1201 ctttcgtcac gacgacaatc aagggtggac gctgactctt cgccgtaaag cgataccctc
     1261 ttgtgatacg ctgcgcagcc tcgtttaata ccgagtcctg gcgaaatgtt gcctgccgat
     1321 ttctaatagc ggccgtctta tctccatctc ctgaggtcac tgtggaaagt gctctacgga
     1381 tacgcacatc ttgagtcggg tcaaataaga acatgaataa gtcggaagca gccaagtgtc
     1441 ccgttaccgg ttgcatcacg tgttcttggc ccggtaagaa actttcgccc gcattatcat
     1501 aaaggcacaa cacccgcccc aaatgggtag ctcctcgggc atttggatga ttagccgtgg
     1561 gctttagcgt aaatataaaa ggcttaaggt attgcacggc ctgccccccg aaaagcacac
     1621 tattgtagaa atcaccgaac gtctcggttt tcggcaaacc tacgatatcg tcctcatttg
     1681 gattcaaaaa aagaaggctc tcatagtcgt tgagtctttg gttaatcaag ggatcagcgt
     1741 cggcaaagga aacgcaaaac tgggaacaca acacggttcg caaggcccac gattgggcgg
     1801 tgataaaata cgacttgcca caggccggtg caccgatgac cgaaactacg cgaggtttca
     1861 actcgaggat ggcccgtggt atctccaagt ggcagtgcgg acatgccgta cggcgacatg
     1921 gcatgccctt tgcatccagg gcagcgcagt caaaagtgaa acgggtgggt aagaagcgcc
     1981 tcggatgttc cgggccgagc ctcggatcac cgaccaagtc ggggtgactg gcaatgtaaa
     2041 gcacgtcgta aactgcgaag tcgcgccaac agtgaggaca aacgattttg ccgcgaacgg
     2101 acgcctgtgg ctgaattgct tggctcatag tcccctctca ttgtgcgatc agagccttat
     2161 caacgtcagg actctcgcgt tggtcaaaag tgctacccac cttctccatt tgctgaagca
     2221 gccggttggc gagagcagcc gcttcgggat cattttcggt aaaaggccca aggctttgcc
     2281 aggcctccaa caagctttgg taacgacgac gagcgtcact ttcattagat tgggttaaga
     2341 catcgcgggc cttttgcacg gcggaattgc gagcggctga cgaacggccg gactcgccgc
     2401 caaaagaccc gtgtccaaca ccctcacccg acgttgccgg ggccgatttc gcgccggtct
     2461 taccgctagg ctggccaggt cctctgtcgc cgagagagcc gtcggactcg acatccgagc
     2521 tgtcgtttcc tattggtcga cgagaagaat ctcggccgtc ccggtgagac gattcggctg
     2581 aggatggagc ctcccgagtg gatcgtcctg ggggaccatt cgagtctgcc tgctgcctgt
     2641 ctttaccacc accctgttct gaggaatctc caacatggct cctgttactg ggctgacctg
     2701 tcggccgccg acaaccgttg acgataatca ggagggatgc agctaccaaa ataaggacga
     2761 ccagccgtga ttcgcggaag agatacattc ctaccccctt tcaccaaaaa agtattgcgg
     2821 gtactttggc acactctatt gaaacgcaaa tgcccggatt ttctaactcg gaacccatcg
     2881 cgtttctcat gtattaccga atatggacgg gtatttggga gctgacctcc tcggccgtgt
     2941 gtcgaaaaag ctactctcgc ccgtcccact cgcggtgcca aatgtattta caagcatagt
     3001 aatatggtaa acacggacaa ctcggccggt caagcatacg gagtccctgg ggttgccggg
     3061 ttctcgggta cgttccatga cggctggcag gtgcagcccc gaagggcgat tcactccgcg
     3121 cctacgcatc tcggaccctg ccagcgcgtt ttaaagttct ctcgcgcttc ttgcgagatt
     3181 cggttcccga ttaactcccc ttgagtgagc aactcgcggg gaatcccgag gtcgggttaa
     3241 gcaagcgatt ctttctctcg gacgtgcggt tgcggctcgt agacgatttc catccaccgt
     3301 ccgtcggcgg caatggacat tcgggccgct tcagtaacta attcgttgat ccaactgttc
     3361 gccggagtgg caaggatacc ccgttggtca atttcgcgca gtacgtgttg ccgggcggcc
     3421 aaggccgctt ctccgtccaa tcccgcagca gccgcattga cccgaagtgc cgcttccaca
     3481 atcgcacaga tcgagtcgta gccgtacccg actgggcgca aaccttcccc ctcccaaggc
     3541 accagccgca tatagtccgg actcacatat cggaacatcg ctcccgaccg cgtgtctgta
     3601 tagccgtggc taacgcctcg atactggtca tcatgatgaa gaatgccgcc gcagtcgggt
     3661 ccttcgcagt acatggtgag gccctggtca ttggtgccgg cgccggcatc agggtatccc
     3721 aatccgttca gtagagtcat agcggcccca ttttcccaaa tcaccgtgcc cgccgaccaa
     3781 agccaaccga tattaccatt agggaaccga ccttcgactc cgcggaccga aacctccacc
     3841 ggacgcagcc ccgtgatgaa atacaccaaa tctacgtaat gacagcccac ataggtaaac
     3901 ggatcactgt tctccttggt gaaccaattc tggaaattgg agtgacggta gtaatagggt
     3961 tcgatcaacc gggcttcgcc caaccggaat tcgccgaacc gtcctttccg atactgccaa
     4021 cgggcatcca aggaacgtcg gtcgaaccgt ttgtggtatt cgacggccac aagaagcccc
     4081 cgtttacgtg ccaattcttc aatggcaacg gcttgccggt aggtgggcac taaaggcttg
     4141 acgcacaaca catgttgatc ccgctcaatg gcctcgcgga taaccatatc gtgtaaatgg
     4201 tccggcaccg caaccaccac aaggttcctc gccggcaggc ggccgattac ctctcgaaac
     4261 agatcgggaa agaaacgatc cggcggctcg gcgagggagg gaaacggctc gaacgtgtgc
     4321 cccggaaatg cttccagtaa tcgttccgag cgagctaacg ccacaagcgg cgccgaattc
     4381 aacgcacaaa tcttgatact tcctaccttg cctaaccgtt ggagatgata caaagacggc
     4441 aagatctgat catgtgtgat catcccaccg cctacgatga caacttctag tttttctgac
     4501 gactttgaca tctgttcggc tccttgatcg ttcaaaggtc tcttgcagtt accacctcat
     4561 tccttggctg atggtgccca cgatatagcg ctaaagagta actgtttccc tac
//
LOCUS       DAOPTR010000162         4611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_15116_length_4611_cov_4.092186, whole genome shotgun sequence.
ACCESSION   DAOPTR010000162 DAOPTR010000000
VERSION     DAOPTR010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15116_length_4611_cov_4.092186"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(346..879)
                     /locus_tag="PKI05_04410"
     CDS             complement(346..879)
                     /locus_tag="PKI05_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51473.1"
                     /translation="MNWKLIFCNPSPLPAQKQIPVSLLENPTVSGRVLYEMARQLYQS
                     QEESLRSLQTRASHLLNVSILGLTGGIFTFLNLSSTCWWAATFSLFFLILACVILLLA
                     LRPIPYAEHFSPWCVTEIPDLVNSEDRFLLWLALSYDVLIQSANQALKTIAARVFLAT
                     FLLIVGLVLFVVTMIVR"
     gene            2041..3462
                     /locus_tag="PKI05_04415"
     CDS             2041..3462
                     /locus_tag="PKI05_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HOA51474.1"
                     /translation="MWTSAQRPATLKELRESGWRSKTVKEELRDNFIRALQSGEDLFP
                     GIIGYDDTVIPEISLAILAGHDMLFLGEKGQAKSRLMRLLTRFLDPAIPYLDIPDVPV
                     HEDPYRPITRRAKEFLAETPEDKVPIAWWPREQRYAERLAPGTKFADIIGEIDPAKLA
                     RGTSLSAEEALFFGLIPRMHRGIFAVNELPELDELVQVGLFNILEERDVQIRGYPIRF
                     DLDILILFSANPATYNRSGKVIPQLKDRIGSVIHTHYPRDRDIAIQITEQEAKIDLNG
                     AYPVVVPYFMKEIIEQISIVARRSKYVDQQSGVSARLSIANYRTMVASARRRGIILGE
                     KPAVPRISDLGHLASSSLGKLELDLMAGHQMTEKQVLDAIIAEAIKDIFQEYVQEHGL
                     AEIAEIFGRGVKIEVGSMLPSAYYAERLKRVPPAWEKAFEINVSNDPAVRASCVEFIL
                     AGLYVTDRISRAEYRGKIVYEIS"
     gene            3449..>4611
                     /locus_tag="PKI05_04420"
     CDS             3449..>4611
                     /locus_tag="PKI05_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51475.1"
                     /translation="MKSPKHLGGVIHVYQKYNPLRFPSPTKPPPDLASAAVNHMLTFG
                     SLRELSEEELAQAIELDPSMIAGLGPSLESLMEMLRQRKAKILATYEVETVHALAARR
                     YRELGSSLKPPQKLSRRYERAFREEQIFELERLWYLCETENSEFARKVLHLVQALGDK
                     YLIDELAAKYHFTGQRPMTIPEALEIKDILETIDRLLEQLQEASKHARLAVIDLEALR
                     RFVEEYDIERLKGFMRQIEDLIRQIAEEQGLERTAQGYQLTPKAYRLFQGRLLEKIFS
                     QLQAARSGRHTGPVIGDGNVELPQTKAYEFGDSLAHMDIPGTMINAMIRQGPGIPVYL
                     HPDDIVVHKTRNVPKCATVALLDMSGSMRYGGLYVDVKRMGLALDGLIRKEYPG"
BASE COUNT         1063 a         1209 c         1291 g         1048 t
ORIGIN      
        1 gctgctcggt caaatgcccc ggccttcctg atgaaatgat tttgcggcgg ttgcaaaaca
       61 ttcgaaaacg cggcccagcg taattgcatt tggcccggac aagaggcact tacgcttctt
      121 ccttatgttg cctaactaag gaaacatttc ttcgtttgcg gaatctccac agacgaaatt
      181 tgccccccgc tttcattggc cgcatcgcta gccagtctat tcaatcaagt cttccacaaa
      241 acgtctcatc ctcgcttgcc agccggttcg gtcccgatcc ccgtcggtta gagcgcgatc
      301 ctcgtcggtt agggtgcgat cctcgtcggt tagggtgcga tcctctcacc gaacgatcat
      361 ggtcaccaca aacaggacaa gcccaacgat tagaaggaag gtggcaagaa aaactcgggc
      421 ggcaatggtc tttagggctt gattggccga ctggatgaga acgtcatagc tgagggctaa
      481 ccaaagcaaa aatctgtcct cggagtttac gaggtcaggt atttcggtaa cgcaccaggg
      541 tgagaagtgc tcggcgtagg ggatgggccg cagggccaag aggagtatca cacaggcgag
      601 aatcaggaaa aagagtgaga atgttgcagc ccaccagcac gtggacgaga gattcaaaaa
      661 ggtgaagatg ccgcccgtga ggcccaggat tgaaacgttt agcaaatgcg acgcccgtgt
      721 ttggaggcta cgcaggcttt cctcttggct ttgatagagt tgacgggcca tttcgtagag
      781 gacacgcccg gagacggttg gattttctag gagtgaaaca gggatttgct tttgcgccgg
      841 cagcggagac ggattacaga agatcagctt ccagttcatg gcgaaactcc ttatgggacg
      901 ttttagggca ttatacgaac gagcatgaaa tcttggcatg tgcccggttc gatgagggcc
      961 gagagcgcta gcttcccaag ctgggggtcg cggggtcgaa tccctggctt ctgctctccc
     1021 tgaggggaac cagactggag atgtggtgcc ccgaaagggg ttgcgggttc gagtcccgtg
     1081 ccctccgctc ggaaatctat ttaaagccca tcccaaaaag tcaccctctc cctcggggag
     1141 aggctggggg cgtgggcagg cgaacgtcgg aatcgactga gaaaactggc agcgattcga
     1201 gtcagcttcg ccccgggccc ctttcccgcc gaacgggcga agggagatta ctggataggt
     1261 tcttggttcg ccacaacggt agatttgggc ctgcgatcac ggttgcgacg cttctgctgt
     1321 taaccccgtg tcgttaggta tatcggcggc ccggttgaat tgggatcaaa accgctgatc
     1381 actctcgaaa ctcagctctg ataagtccct cttttcggaa gccgacccag ggaattccag
     1441 tggtagccag tgtctccgaa acgagaccaa tttcgtacac cacgcccggc gttgattcca
     1501 tccggtgacg gcctcttcgc tctgtactag gtcttctggt accccttgtg aaatcaaatc
     1561 ctatctgtca cggttttttt cgatttacct aatgtgcatt gacctcgcga cccgcgcttg
     1621 acggtgtggt cctcctcggg ggcatgtcct cccgtacaac gtagtgacga gggccgcgga
     1681 gcttttggct catattgtgc ttgcgcgcga tggatgtcgt tgccctttgt taaaattcgc
     1741 tccccctttt tcgacgattg tgcaccgcac aagtcttcca ttcacgtctg cgttgccaca
     1801 aaccatattc atcgcgtggt cactagttgt ccattccgac atgccagccg gcaaattcag
     1861 ccgcagcaac ggtcgagggt atcgtgcgct tggtggatca actggggtgg aggtgcagta
     1921 tgggtgcact taacccattg ccattccgcg ctacaatgaa agcgggtcaa catcccaggg
     1981 gtgattaaga aatcttgtgt gcgagacagc tgcccgcgct gaagaaggag cggaacaacc
     2041 atgtggacct cggcccaaag gccagcaacg ctgaaagaac ttcgggaaag cggatggcgg
     2101 tcgaagacag ttaaggagga attgcgcgac aattttatcc gggcgcttca atcgggcgag
     2161 gatctgtttc ccggcatcat cggctatgac gataccgtca ttccggagat cagtctcgcc
     2221 attcttgcgg gtcacgatat gctgtttctg ggtgaaaaag gccaagccaa aagccgtctc
     2281 atgcggttgc ttaccaggtt tctggacccc gccatcccgt atcttgatat ccccgacgtg
     2341 cctgttcacg aagacccgta tcgtccgatc acgcgccgag caaaagagtt cctggccgag
     2401 acaccggaag acaaggtccc cattgcttgg tggccccggg agcagcgcta tgcggagcga
     2461 ctggcaccgg ggacaaaatt tgcagacatc attggcgaaa tcgaccctgc caaactggcc
     2521 aggggcacca gtctttcagc cgaggaggcc ttgttctttg gattaatccc gaggatgcat
     2581 cgcgggatct tcgccgtcaa tgagttacct gaattggatg agctagttca ggtgggcctt
     2641 ttcaatatcc tggaagagcg ggacgtacag attcgtgggt atcccatccg gtttgatctg
     2701 gatattctca tcctgttttc cgccaatcct gctacttaca accgaagtgg taaggttatt
     2761 ccgcagttga aagaccgcat cggttctgtg atccatacgc attatccgcg tgaccgcgac
     2821 attgccatcc aaatcaccga acaggaggct aagatcgatc tgaacggcgc gtaccccgtg
     2881 gtggtccctt atttcatgaa agagattatc gagcagatca gtattgtcgc gcggcgatcg
     2941 aaatatgtgg accagcaatc gggcgtcagt gcccggttga gcattgccaa ttaccggacc
     3001 atggtggcga gcgcccgccg tcgtggcatc atcttgggtg aaaaaccagc tgtccctcgc
     3061 attagtgatc ttggtcactt ggcctcgtcc tcgctgggaa aactggaact agacctgatg
     3121 gcgggccacc agatgaccga aaaacaagtt ttagacgcta tcattgcgga agcgatcaag
     3181 gatatctttc aagagtacgt gcaggagcat ggccttgccg agatcgccga gatttttggg
     3241 cgaggcgtga aaattgaagt gggcagcatg ttaccctcgg catattacgc ggagcggctg
     3301 aaacgggtac cccccgcctg ggaaaaggct tttgaaatta acgtctcgaa cgatcccgcc
     3361 gtgagggctt cctgcgttga gttcattttg gcgggattat atgtgaccga ccgcatttcg
     3421 cgggccgaat atcgtggaaa gatcgtctat gaaatctcct aagcatctag gcggtgtcat
     3481 tcacgtttat caaaagtaca acccgttgcg gtttcctagc cccacgaaac cgccgccgga
     3541 tttagcgtcg gccgccgtca atcacatgct tacgtttggt tcgctgcggg aattgagcga
     3601 ggaagaactc gctcaggcca tcgagcttga cccaagtatg atcgccgggc taggcccttc
     3661 gctggaatcg ttaatggaaa tgctccgcca gcgtaaggcc aagattcttg cgacttacga
     3721 agttgaaact gttcatgcct tggcagcccg tcgttaccga gagttgggaa gtagcctaaa
     3781 gccaccgcag aaactatctc gccgctacga acgggcgttt cgcgaagagc agatctttga
     3841 attagagaga ctttggtacc tatgtgaaac cgagaacagt gaattcgcac gaaaagtgct
     3901 tcatttggtt caggcactcg gggacaagta ccttattgat gaactggcgg ccaaatatca
     3961 tttcacgggg caacggccca tgacaatccc cgaggcccta gagatcaaag atattctgga
     4021 gaccatcgac cgccttcttg aacagcttca ggaagcctcc aagcatgcgc gcttggccgt
     4081 cattgatctg gaggccctgc ggcggtttgt ggaagagtac gacatcgaac gtttgaaggg
     4141 tttcatgagg caaatcgaag atctcattcg gcaaatcgcc gaggaacaag gtctggaacg
     4201 aactgcccag ggctaccaac tgacgccgaa ggcgtaccga ttgtttcaag ggcgactctt
     4261 ggagaagatt ttcagccaac ttcaggccgc ccgcagcggc cgacacacag gtcctgtgat
     4321 cggagacggt aatgtcgaat taccccaaac caaagcgtac gagtttggcg actcactggc
     4381 ccacatggac attcccggaa caatgatcaa cgccatgatc cgacaggggc caggtatccc
     4441 cgtttatctt catccagacg acattgttgt ccacaagacc cgcaatgttc ccaaatgtgc
     4501 cacagtcgcc ctgttggaca tgagtggctc aatgcggtac ggcggcctat atgtggacgt
     4561 caagcgaatg gggttggctt tggatggact gattcgaaag gagtatccag g
//
LOCUS       DAOPTR010000163         4610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_15127_length_4610_cov_3.887377, whole genome shotgun sequence.
ACCESSION   DAOPTR010000163 DAOPTR010000000
VERSION     DAOPTR010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15127_length_4610_cov_3.887377"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PKI05_04425"
     CDS             <1..682
                     /locus_tag="PKI05_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOA51476.1"
                     /translation="GHYALASECYCHFTSPIRRYPDLTVHRLVDAMLLNFKPRINPTE
                     LVALGEHCSEREERGEAAERELTNLKLLSFLSERLGMELDGIVTGVEKYGLFVQGIAL
                     PADGFIPVEALSDDYYHFDRASHSLCGRRSGNQFRLGDLVRVAVARVDLERRQLDFRL
                     LAHQGHPGPNTVAESLTSFGSAERDRKKDKAGKTRHVSLSERVRLQGLRKLQSKKQSA
                     KKKSQKRR"
     gene            758..1813
                     /gene="ltaE"
                     /locus_tag="PKI05_04430"
     CDS             758..1813
                     /gene="ltaE"
                     /locus_tag="PKI05_04430"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HOA51477.1"
                     /translation="MTSNKLLDFRSDTVTRPTPGMRAAMAAAEVGDDVLGDDPTVHRL
                     EERVAEILGKEAAVFVPSGTMSNLIAVRVHCRPGDEMICEADAHIYNYEQGGYAQICG
                     VAVRPVQGEWGLIRLEQVEGLVRPNNAHFVRTRLLTLENTHNRGGGRVQPYPLVESLT
                     AWARQSNLRTHLDGARLWNAYVATGISPATWAQHFDTVNVCFSKGLGAPVGSALAGPR
                     DLIAEARRHRKVLGGGMRQAGILAAAALYALDHHIERLAEDHHNAKRLAEGLAHIPFV
                     TVMPIPVETNILLFDIDRAWGSAAAFSAVLKEHGVLINATAPQRLRAVTHLDVTAADV
                     DKALAVLDHVAREHRGK"
     gene            complement(2160..3992)
                     /gene="ilvD"
                     /locus_tag="PKI05_04435"
     CDS             complement(2160..3992)
                     /gene="ilvD"
                     /locus_tag="PKI05_04435"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF009103.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOA51478.1"
                     /translation="MRSDQIKLGAARAPHRSLLRATGLKDEDFRKPFIAICNSYTDVI
                     PGHVHLHAVGEFIKECVRAAGGVPFVFNTIGVDDGIAMGHTGMKFSLPSRELIADCVE
                     TMIQAHCFDGMICIPNCDKIIPGMLMAAVRCNIPTIFVSGGPMEAGRTPEGKKVDLID
                     VFIGAAARQDGKLTDEELAELEKLGCPTCGSCSGLFTANSMNCLCEALGIALPTNGTL
                     LATSQERKRLYQRAAYRIVEMVLQFEKLGPGHGLLPREIITRESIDNAMILDMAMGGS
                     TNTVLHMMAVAQEAEVDYDLRRIDELSRRTPNICKVSPSSRYHIEDVHNSGGIHTILG
                     AIQRGRPGLLHLECPTVTGKTLGENILEYDIRNPQVSEEALELAAVTASGQINAPGTS
                     VPRKFRSVRELTPEQLGFDPYDCIREVNNAYNQEGGLAILFGNLAPNGAVVKTAGVLP
                     HMLQFVGPAVIFESETEAYQGIINGKVKAGDVVVIRYEGPRGGPGMQEMLAPTTALKG
                     VKLDDKVALITDGRFSGGTAGACVGHVSPEAAAGGPIGLLQPGDVIEIDIPARKLNVR
                     LSEEALAERRKRWTPPPPRFTRGILARYAKLATSADTGAVLRLD"
     gene            complement(4168..>4610)
                     /locus_tag="PKI05_04440"
     CDS             complement(4168..>4610)
                     /locus_tag="PKI05_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOA51479.1"
                     /translation="KRAKLAYEMMGWSLPDSPDSYLRMIKAVDREAFAVHLDPCNLIN
                     CPERFYNNTALLNECFDKLGPWIVSCHAKDLKWEVEMNVHFVEVPLGQGSLDYRTYLQ
                     RLATLPADVPLMMEHMKTAEEYQLSQEYLKKVGLEIGVLFEYVG"
BASE COUNT          965 a         1380 c         1293 g          972 t
ORIGIN      
        1 aggacactat gcccttgcca gcgaatgtta ttgtcacttc acgtcgccca tccgccgcta
       61 tcccgacttg accgtccatc gcctggtgga tgcgatgttg ttgaacttta agccgcggat
      121 caaccccacc gagctggtcg ccctgggcga acactgctcg gaacgcgaag agcgcggcga
      181 ggccgccgaa cgggaactca cgaatttaaa actattgtcg ttcctcagcg agcgcctcgg
      241 gatggagctg gacggtatcg tgacgggtgt ggagaaatac gggctatttg tgcaggggat
      301 cgctttacct gccgacgggt tcatccctgt ggaagccctc agcgacgatt attatcattt
      361 tgatcgggca agccattccc tatgcggacg acgatcgggc aaccagttcc gcctaggtga
      421 cttggtccgt gtggccgtgg cccgcgtcga tttggaacgc cggcaactgg attttcgtct
      481 cctggcccat caggggcatc ccggaccgaa cactgttgcc gagtctctca cgagttttgg
      541 gagcgcggag cgggaccgca aaaaggacaa ggcaggcaag acacgccatg tatccctttc
      601 ggaacgagtg cggctccaag ggcttcgcaa gttgcagagt aagaaacaat cggctaagaa
      661 aaagtcccaa aaacggcgtt gagtaaaaat aatcgtcctc gcgtcacggt tttccactgc
      721 ttggactaaa tgaatcattc tcgggtagcg acggatcatg acgagcaaca aactcttgga
      781 tttccgtagc gacacggtca caaggccaac cccggggatg cgtgccgcca tggccgccgc
      841 cgaggtcggc gacgatgtcc tcggtgatga tcccaccgtg cacaggttgg aagagcgtgt
      901 ggcagaaatc ctgggaaaag aggctgccgt cttcgtcccc tcgggaacca tgtcgaatct
      961 catcgcggtt cgcgtccact gccgtccggg tgatgagatg atctgcgagg ccgacgccca
     1021 tatttacaac tacgaacagg gtggctacgc ccagatttgc ggcgtggcag tccgaccggt
     1081 tcagggcgag tggggattaa ttcggctaga acaagtggag ggacttgttc gaccaaacaa
     1141 tgcccatttc gttcgcacgc gccttttgac cctggagaat acccacaacc ggggtggcgg
     1201 gcgtgtccag ccgtaccctc ttgtagaatc gctaacggcg tgggcacgtc agagcaatct
     1261 gcgcacccac ctggacggtg cccggctctg gaatgcctac gtggcgacgg gaatcagccc
     1321 ggccacctgg gcccaacact tcgacaccgt caacgtctgt ttcagtaagg gattgggggc
     1381 gcccgttggt tcggctttgg ccggcccgcg ggatttgatc gccgaggccc gccgtcaccg
     1441 taaggtcttg ggtggaggaa tgcggcaagc tgggatactg gcagcggccg ctttgtatgc
     1501 cctcgatcac cacatcgagc gacttgccga ggatcaccac aatgccaaac gtcttgccga
     1561 gggtctggca cacatcccct tcgtcacggt gatgcccatc cccgtggaaa cgaacatcct
     1621 ccttttcgat attgatcggg catggggtag tgcggcagca ttctctgctg tgctgaaaga
     1681 gcatggcgtc ctcatcaacg ccacggcgcc acaacgactc cgcgccgtta cccatcttga
     1741 cgtgacggca gcggacgttg acaaggccct cgccgtactt gaccacgtag cccgggaaca
     1801 tcgcggcaag tgagcgccat cgccgcaacc acaatagctt gacagacctt tgggggccgt
     1861 cgccaacctg cgacggagaa agccgtcaag ctctgtgacg cttcgtgtac gggtcgcaag
     1921 ctctcaggcc gaagagtttc ctgcgtcgtg acagccggcc ccttttctgg tggctaagag
     1981 ccgatcccat aatctcccct cacccgctcg gcgcgagagg agtcggggat gagggtggct
     2041 cgaatcgctg ccggctttct caatcgattc cgacgatcgc ctcccctcac cccaaccctc
     2101 tcccagaggg agagggggac tttttgggat agactcttat tccgcggcgt tcggggcttt
     2161 cagtccaagc ggagaaccgc gcccgtatcg gcactggtcg caagtttggc atagcgggcc
     2221 aagatacccc gtgtgaatcg aggtgggggt ggtgtccacc gctttcgtcg ctcggcaagg
     2281 gcctcctcgc taagtcgaac gttcaatttt ctcgccggaa tgtcaatctc gatcacgtcc
     2341 ccgggttgga gcagaccaat cggtcccccg gcagcggcct ccggactgac gtgtcccaca
     2401 catgccccgg ccgttcctcc agaaaaccgg ccatcggtga tcaaagccac cttgtcatcc
     2461 agcttgacgc ccttgagcgc ggtcgtcggc gccagcattt cctgcattcc cgggcccccg
     2521 cgtgggcctt cgtaacgaat cacgacgaca tctccggctt tgaccttccc gttaatgatt
     2581 ccctggtagg cctccgtttc tgattcaaag atcacagccg gaccgacaaa ctgcaacatg
     2641 tgcggcaaga cgcctgccgt tttcacaacc gcaccgttgg gggccaggtt accaaacaag
     2701 atcgccagac cgccttcctg attgtaggcg ttgttcacct cacggatgca atcgtagggg
     2761 tcaaacccca attgctcggg cgtcaactcg cggacagatc ggaatttgcg gggcacactc
     2821 gtacctgggg cgttgatttg cccactggcc gtgacggcgg cgagttccaa ggcctcctca
     2881 ctgacttgag gattgcggat atcgtactcg aggatgtttt ccccgagggt cttcccagtc
     2941 accgttgggc actcgaggtg caaaagccct ggtcgtcccc gctgaatggc tccaagaatc
     3001 gtgtgaatcc cgccggagtt gtgcacatcc tcaatatggt atcgactgct cggggacacc
     3061 ttgcagatat tgggggtcct gcggctcaac tcgtcgatcc gccgcaggtc gtagtcgacc
     3121 tctgcctcct gggccacggc catcatgtgc aggaccgtat tcgtgctacc gcccatggcc
     3181 atatccagaa tcatggcatt gtcgatggat tcacgagtaa tgatctctcg tggaaggagc
     3241 ccatgaccag ggccgagctt ttcaaactgg aggaccattt ccacgatccg ataggcggct
     3301 cgctggtaga ggcgcttccg ctcttggctg gtggccaaca gggtaccatt ggtgggcaag
     3361 gcgataccga gggcttcgca aagacagttc atactgttgg cggtgaataa cccagagcac
     3421 gacccacagg taggacagcc cagcttttcc agctcggcca actcttcgtc cgtcaatttg
     3481 ccatcttgtc gggcagccgc gccgataaag acgtcaatca agtccacctt ctttccctcg
     3541 ggcgttcgac cggcctccat gggaccgccg ctgacgaaga tcgtcggaat gttgcaacgc
     3601 acggcggcca tcaacatgcc ggggatgatc ttgtcacaat tggggatgca aatcatgcca
     3661 tcgaagcaat gggcctggat catcgtctcc acgcagtcgg cgatgagttc ccggctgggg
     3721 agcgaaaact tcatgccggt gtgccccatc gcaatgccgt cgtccacccc aatggtgttg
     3781 aagacaaaag gaacgccccc ggcagctcga acgcactcct taatgaattc ccccacggcg
     3841 tggagatgaa cgtgaccggg aatgacgtcg gtataactgt tgcaaatggc aataaatggc
     3901 ttgcgaaaat cctcatcttt caggcccgtc gcgcgcaaaa gactgcgatg aggtgccctc
     3961 gcagctccca gcttgatttg atcggaacgc attatctctc cctcgctcga tggacagaac
     4021 aacgtcaatc gtgccttttt taaatagccc aacaaacggg ctactgcaaa gaccaaacgc
     4081 tttattctat tcctccccaa cacaatcaac cagccgcggc attttggagg acaatcacca
     4141 acgccggtgg ggaccggtat ttcgttgtca tcctacgtac tcaaaaagca cgccgatctc
     4201 caggcccacc tttttcaaat actcctgcga aagttggtat tcttctgcag tcttcatgtg
     4261 ttccatcatg agggggacgt ctgcgggcaa ggtcgccagc cgctgcaaat acgtgcggta
     4321 atccagggaa ccctgtccca gtgggacttc gacaaagtgg acgttcatct cgacttccca
     4381 cttaaggtct ttggcatgac agctcacaat ccacgggcct agtttgtcga agcactcatt
     4441 gagcaaggcc gtgttgttgt agaaccgctc aggacaattg atgagattgc acgggtctaa
     4501 atggacggca aaggcctcgc gatccaccgc cttgatcatt cgcaggtaac tatccgggct
     4561 atccggcaat gaccagccca tcatttcata ggccagctta gcccgtttgg
//
LOCUS       DAOPTR010000164         4587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_15215_length_4587_cov_3.407105, whole genome shotgun sequence.
ACCESSION   DAOPTR010000164 DAOPTR010000000
VERSION     DAOPTR010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15215_length_4587_cov_3.407105"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="PKI05_04445"
     CDS             complement(<1..1323)
                     /locus_tag="PKI05_04445"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOA51480.1"
                     /translation="MGTLLAISVFAPLAGVLIVGDSQKVARRVTLIVVLLTTALSGWI
                     AFRFPVGAQQFAVFEVPWWSRSDQTIGIRFALGLDGLSVWLYALTSLLMVTSVLVSWN
                     AIDRQAPTFYRLLLVLYTGMLGVFTARDLLLFYVFFEATLIPLFFLIGIWGHEERRHA
                     AIKFFLFTLAGSVLTFLGLLAVVIWTASYSGTGRMTFSIPELTTRLFELRSQGLFPLS
                     VQLWIFWALFLGFAVKVPLVPLHTWLPLAHVEAPTAGSVLLAGILLKVGGYGFLRLVL
                     PIVPDAVRVLAPWLLTLCVVGIVYGAFTAFAQRDVKRLIAYSSVSHLGFCMLGVFCLS
                     PVGLHGGLLQMINHGLATGGLFAVVGMLYERYHTRKIEAFGGLARKLPILAFMTFVLT
                     FSSIGLPGLNGFVGEFLVLLAMFQRAWSHGGGELALAILATSGVVLGAW"
     gene            complement(1426..3483)
                     /gene="nuoL"
                     /locus_tag="PKI05_04450"
     CDS             complement(1426..3483)
                     /gene="nuoL"
                     /locus_tag="PKI05_04450"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOA51481.1"
                     /translation="MTFLIILIAAMPLGAAILTAVLGPRLLREFSHVPAVTATAISFI
                     ASLALLLLVGQTLAQHSADGEQRVLERVVQLWSWADISPTGGSPQVPVAHGTVDFVRF
                     QIPVVLRVDPLTVFMLVTVTAVSLLVVIYSIGYMHDDPGYWRFFTYVSLFIFSMIMLV
                     SVSNFLLLYIFWEAVGLCSYLLIGFWYRKPAAAAAGKKAFLVNRIGDFGFALGIFLIW
                     SVYGTLNFHDTIDPETGEVYSAGVLGMARLVHPEQGLVHGAVATAICLLLFAGACGKS
                     AQFPLHVWLPDAMEGPTPVSALIHAATMVTAGVYMVARCMPLFEAAPYALPIVGIIAI
                     ITATMAAIIALTQTDLKRILAYSTISQLAYMFLGLSVGGLVGVAAAMFHLFTHAFFKA
                     LLFLGAGSVMHATGGVIDINEIGGLRRRLPWTHATFLVGSLALAGLCPFAGFFSKDEI
                     LHLVYEKGSASSLYFFLFVAAAVTAMLTAIYTFRAYFRTFFGEERVPAEAAGHLHESP
                     PVMVIPLVILAIASAVVGVAFGPGKVIEHFLAATPSLAAFAAHEVGAHHGGGAALIPW
                     LSTALTLGGIGWAALVVLAPGGVARPVIALTKAIGLYALSFHKFFFDELYTWIIVRPL
                     TWLAAVAAWIDRYFIDLLVDIIGYLPKLVGALLRSSHNGWIPWYGLGIVLGVLVLLAA
                     MFF"
     gene            complement(3489..3932)
                     /gene="nuoK"
                     /locus_tag="PKI05_04455"
     CDS             complement(3489..3932)
                     /gene="nuoK"
                     /locus_tag="PKI05_04455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOA51482.1"
                     /translation="MENTGLLMNYLLVGTCLFGLGVVGFLARRNLIVMFLSVELMLQG
                     VIVNFAGWDRYHGQATGQVFVLFIVGVTAAEAAVALALILTLVRAREGLDVLRLQKVR
                     EDIILPPVPDEGEVREAETPSWPRLPRVGRRPDIPQEVLEYRPSV"
     gene            complement(3963..>4587)
                     /locus_tag="PKI05_04460"
     CDS             complement(3963..>4587)
                     /locus_tag="PKI05_04460"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOA51483.1"
                     /translation="AMLSAMIPSVGSLLARSLLTLMSIVAVGSAVAMITSSRPLYAAL
                     WFGMAVLGTAGMLLFNGAQFVAMAVIVVYIGAILVMFLFVLMLDDPKGTAGFNRQSWE
                     PLTASVVGAVLIALTTVTWLRHAATANGWSLAKETGKAIPDVVITRTVDLGNQLFLHY
                     GVAVQALAIVLLLGLLGAAVIIAHPLEPLLDFKSRAVPDETPTANES"
BASE COUNT         1267 a         1339 c         1244 g          737 t
ORIGIN      
        1 ccacgctcct aggacaactc ccgacgtggc caatatggca agggccaact ctccgccgcc
       61 atgggaccac gcccgttgga acatggcgag aagtacgagg aattctccta cgaaaccgtt
      121 aaggcctggt agcccaatac tggaaaatgt caaaacgaat gtcatgaaag cgagaatggg
      181 tagctttcgc gccaggccac caaaggcctc aatcttccgc gtgtgatacc gctcgtagag
      241 catcccgaca acggcaaaca ggccaccggt agccagtcca tggttgatca tctggagaag
      301 accaccgtgg agccccaccg gactaaggca aaagaccccc agcatacaga atccaaggtg
      361 actgacgctg gaatacgcga tcaagcgttt gacatccctc tgggcaaatg ccgtaaatgc
      421 cccataaacg atgcccacta cacagagcgt caataaccag ggggcgagga cacgaacggc
      481 atccggcaca atgggcaaaa ccagtcgcag aaagccgtat cctcccacct tcaagagaat
      541 cccggcgagt agcacactcc cggccgtggg ggcttccacg tgcgccagcg gaagccaggt
      601 atggagcggc acaagcggca ctttcacggc gaatccgaga aacagtgccc agaaaatcca
      661 gagttgaacc gaaagcggaa acagtccttg cgatcgcaac tcgaagaggc gagtcgtaag
      721 ttcaggaatg gagaacgtca tccttcccgt tccgctgtaa gaggcggtcc aaatcacaac
      781 ggccaggagc cccagaaacg taagcacact tcctgccagg gtaaaaagga agaacttgat
      841 cgccgcgtga cgtcgctcct cgtgtcccca aattccaatc agaaagaata gcgggattaa
      901 ggtcgcttca aagaacacgt agaacaatag gagatcacgg gcggtaaaaa cccccagcat
      961 accggtgtac aacaccagca aaaggcgata gaaggtcggg gcctggcggt caatggcatt
     1021 ccaactgacc agcaccgatg tcaccatgag cagcgaagtg agggcgtaca gccaaacgct
     1081 caggccgtct aagcccaggg caaagcgaat accgatggtc tggtcggacc tcgaccacca
     1141 ggggacctca aagaccgcaa attgctgtgc ccccacagga aaccgaaagg cgatccatcc
     1201 gcttagggcg gtcgtcagaa ggacgacgat cagcgtcaca cggcgtgcga ccttttggct
     1261 atcgccaacg atgaggacgc ccgccagcgg tgcgaagacg ctaatggcta acagtgttcc
     1321 cattgccaaa cctttcactc ctccgcacaa gcggagcttc taacgaactg accccaaggt
     1381 ccgcaaaatt tcccagcgtt gaacaccacg atcaccacgt tcccattaaa agaacatcgc
     1441 agcgagcaag accaaaaccc cgaggacgat ccccagcccg taccagggaa tccaaccatt
     1501 gtgcgaactt cgcaggagag cccccaccag cttgggcagg tagccaatga tatccaccag
     1561 caaatcaatg aagtatcggt cgatccatgc tgccactgcc gcgagccacg tgaggggtcg
     1621 gacgatgatc catgtataaa gttcatcaaa gaagaactta tggaaagata gcgcataaag
     1681 gccaatcgcc tttgtcaagg cgatcacagg tcgggctacg ccgcccgggg cgaggacaac
     1741 cagcgcggcc catccgatgc caccgagggt tagggccgtg ctcagccacg gaatcaaagc
     1801 ggcaccacca ccgtggtgcg ccccaacctc gtgtgcggca aatgcggcaa ggctcggtgt
     1861 cgccgcgaga aagtgctcga tcaccttacc aggaccgaac gcgaccccaa caaccgccga
     1921 ggcaattgcc aaaatgacga gggggatcac catgacaggg ggcgactcgt ggaggtggcc
     1981 ggcggcctct gcagggaccc gctcttcacc aaaaaacgtg cgaaaatatg cacggaaggt
     2041 gtagatggcc gtcaacatag cagtaacggc tgccgccacg aacaggaaga agtaaagcga
     2101 tgaggcactt cccttttcat aaacgagatg gaggatttca tccttactga agaatccagc
     2161 gaacgggcac aatcctgcca aggccaaact gccaacaagg aacgtggcgt gggtccaggg
     2221 caatcgacgc cgcaatccgc caatttcatt gatgtcgatc acgccgcctg tggcatgcat
     2281 gacactgccc gcccctaaga acaagagggc cttgaagaag gcatgggtaa ataagtgaaa
     2341 catcgcggca gcgacaccta ccaacccacc gacggacagc cccaagaaca tgtaggcaag
     2401 ctggctgatc gtggaatagg ctagaatgcg tttgaggtct gtctgggtca gcgcaatgat
     2461 ggccgccatg gtggccgtga tgatggcaat gatccccaca attggcaggg catagggtgc
     2521 agcctcgaac agtggcatac atcgtgcgac catataaaca ccggccgtca ccatggtggc
     2581 ggcgtgaatc agggcactca cgggcgttgg gccttccatg gcatcaggaa gccacacatg
     2641 taaaggaaat tgagcgcttt ttccgcaggc ccctgcgaag aggagcaaac aaatggccgt
     2701 ggccacagcg ccgtggacaa gcccctgctc gggatgaacg agccgggcca ttcccagcac
     2761 tcccgccgaa tagacttcgc cggtttcggg atcaattgtg tcgtgaaagt tcagtgttcc
     2821 atacaccgac cagatcaaaa agatgccgag agcaaatcca aaatcaccga tccgattgac
     2881 aagaaacgct ttcttccccg ccgcggccgc agcgggcttg cgataccaga aaccgatcaa
     2941 gaggtagctg cacaggccca cggcctccca aaagatgtaa aggagcaaga aattactgac
     3001 cgagacgagc atgatcatgg agaagatgaa aagagaaaca taggtgaaga atcgccagta
     3061 gccgggatca tcatgcatgt atccaatcga gtaaatgact accagcaagg agacggccgt
     3121 aaccgtcacc aacatgaaga ctgtcaaggg atcgacgcga agaacaacgg gaatctgaaa
     3181 tcgcacaaag tccaccgtgc catgggctac gggaacttgg ggagacccgc cggtcggact
     3241 gatgtcggcc cagctccaga gttggaccac gcgctcaaga acacgctgtt cgccgtcagc
     3301 cgaatgctgg gcaagagtct ggcctaccaa aagaagcaat gccaatgacg ctatgaagga
     3361 aatggctgtt gccgttacgg ccggcacatg actaaattct cgcagaaggc gcgggccgag
     3421 cacggctgtc aggatcgcag ccccaagggg catcgcggct atgagaataa tgagaaatgt
     3481 catgggggtt acacactcgg tcgatattca agaacttcct ggggaatatc gggacgccgt
     3541 cccacccggg ggagcctcgg ccatgaaggt gtctcggctt ccctgacctc accttcatcg
     3601 ggcaccggcg gaaggatgat atcctcgcgg accttttgca agcgtaggac atccaacccc
     3661 tctcttgccc tcacgagcgt caaaatcagt gccaaagcga cggccgcctc cgccgcggtg
     3721 acgcccacaa taaagagaac aaatacctgg cccgtcgctt gaccatgata acggtcccaa
     3781 ccggcaaaat tgacgataac accttgaagc attaactcaa cggaaaggaa catgacgatt
     3841 agattccgcc gggccagaaa accgacgacc cccaagccga aaaggcacgt ccccaccagc
     3901 aagtaattca tgagtaagcc agtgttttcc atggtattcc cgtatggctt ttgctcctcg
     3961 gcttaggatt cattcgcggt cggcgtttcg tccggcacag cgcgactctt gaagtccaga
     4021 agcggttcca atggatgggc gatgatcacg gcagcgccca gcaaaccaag taagagcaca
     4081 atcgccaaag cctgcacggc cacaccgtag tgcaaaaaca attggttgcc cagatcgacg
     4141 gttcgcgtga tcacaacatc cgggatcgcc ttacccgtct cttttgccaa agaccacccg
     4201 ttagccgtgg cggcatggcg cagccaggtg accgtcgtca gggcaatgag gaccgcaccc
     4261 actacggagg ccgtgagcgg ttcccaactt tgtcgattga aaccggctgt ccctttggga
     4321 tcgtcaagca tgaggacgaa caagaacatg accaaaatag cgccgatgta aacaacaatc
     4381 acggccatgg ccacaaattg cgcaccattg aataacagca tcccggccgt gcccaaaacc
     4441 gccatgccaa accaaagggc ggcatacaac ggacgactgc tcgtgatcat ggccaccgcc
     4501 gagccgacgg ccacaataga catcagtgtg agaagagagc gtgctaacag gctgcccacg
     4561 gacggaatca tagccgaaag catcgcc
//
LOCUS       DAOPTR010000165         4581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_15242_length_4581_cov_3.762483, whole genome shotgun sequence.
ACCESSION   DAOPTR010000165 DAOPTR010000000
VERSION     DAOPTR010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15242_length_4581_cov_3.762483"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..327)
                     /locus_tag="PKI05_04465"
     CDS             complement(34..327)
                     /locus_tag="PKI05_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51484.1"
                     /translation="MTNRVWPKMMSFCALGLTSGISVRWSVLKAVILVGYISVGVFVF
                     LLSGQGLAATVEEGQKPWKPGPRRARHRPAGSGARTHPLQAGPGGQTAPPTTT"
     gene            complement(458..1324)
                     /gene="rfbD"
                     /locus_tag="PKI05_04470"
     CDS             complement(458..1324)
                     /gene="rfbD"
                     /locus_tag="PKI05_04470"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOA51485.1"
                     /translation="MPIAVTGVKGQLGGELCRQLGDRAVGLDLPEFDLLDYDRVRKTI
                     RELRPELLIHAAAYTAVDKAEDEPEKCFAINATATGVLVDLARELDCPFLYVSTDYVF
                     GGEPERSEPFREEDVPSPQGVYARSKYEGELKTLVWEKHYIVRTCGLYGQLGPHSAGN
                     FVETMIRLANEGRPLRVVDDQFCTPSYVVHVARAIIFLIQTDMYGTFHIVNDGATTWY
                     RFAREIFRQLKMDVDITPISTEEYGAKAPRPRFSVLDTSKYNRFFGAPPLPHWKEALA
                     EFLARRAEFLGH"
     gene            1517..2047
                     /locus_tag="PKI05_04475"
     CDS             1517..2047
                     /locus_tag="PKI05_04475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOA51486.1"
                     /translation="MPIYEFYCDKCHVIYNFFSRRINTDKRPDCPKCGRPKLERRVSR
                     FAIGRGGSSESKSGDNAEADDDLLPPGFDEEKFEEAMMELATEAENLDEDDPRQMARL
                     MRRLSQKTGLQFGSAMEEAIRRLEAGEDPETIEEELGDVLEQEEPLFQQSGKSLRTLY
                     EHLKPPRVDEKLYDLD"
     gene            2210..2398
                     /locus_tag="PKI05_04480"
     CDS             2210..2398
                     /locus_tag="PKI05_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51487.1"
                     /translation="MPARERREWAWMSADVWVIEDDDPNSRGGLARDAVPRVRHSGGT
                     PRPDQTKEIAPPHAHPHP"
     gene            complement(2568..3938)
                     /locus_tag="PKI05_04485"
     CDS             complement(2568..3938)
                     /locus_tag="PKI05_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008670506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA51488.1"
                     /translation="MSISRRTTRRRFLQGSVGLGVTAIILSQRRYAVGFESANERPVF
                     ATIGLRNQGWEITQKTFKFADFAALADVDAKVLAENVQKTEQRQGKKPDAYKDYRYVL
                     DRKDIDAVMIATPDHWHTKIAVEAMYAGKDVYCEKPLTLTIDEGKLIEKVVKETGRVF
                     QVGTMQRTECDKRFLIAVALVKHGRIGKVRKVTCGINGMEPSPPIPEAPVPEGLDWDF
                     WLGPAEKVPYRALPELRTGYGGGVPYYSNCHYSFRNWHEYSGGKLTDWGAHHVDIAVW
                     AIGATDTGPSKVTPLNYTLACEYKDGYPVVHDRYNVATQFNIRAEMPGDIELIITSEG
                     DNGILFEGTEGRFFVNRGRITGKPVEELQDNPLPEDALEQIYGGPISANHSANFIEAM
                     RARKQPISDVWSHNRMLEICHLANIAMRLGRELRWDPVKREIVGDPQANSFLSRPSRK
                     GYEIKI"
     gene            complement(4295..>4581)
                     /locus_tag="PKI05_04490"
     CDS             complement(4295..>4581)
                     /locus_tag="PKI05_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51489.1"
                     /translation="QKTYILGVTTHVKKTCCMDRQVNKIIAWFIHENNYSYLPSVRAD
                     SGGNHRGLPVRLMPFPSRRVIEITWHGHLARVIVRHGVERLSPTGIGVVQ"
BASE COUNT         1021 a         1319 c         1293 g          948 t
ORIGIN      
        1 tgatggacga ggtccgtccg ctggaccagt ttgtcaagtt gtcgttggtg gagctgtctg
       61 ccccccgggt ccggcttgca agggatgcgt ccgggcgcct gaacctgcag ggcggtgccg
      121 cgcccgccgc ggccccggct tccacggctt ttggccctcc tcaaccgttg ctgccaaccc
      181 ctggccggaa agcaaaaaaa cgaacacgcc gaccgaaata tatccaacca ggatgacggc
      241 cttcagcacg gaccatcgca ccgagatacc gctggtcaac ccgagggcac agaatgacat
      301 catcttgggc cagacgcgat tcgtcatggc aaggctcccc taaataccat gcgggacttt
      361 ggccgggacc aggcaggtcc gcgggcagga acaatcaaat gcctgttcag tttgacggac
      421 ggcaaagtaa aaatcaagcg cagtcgccgc agccgcctca atgacctaaa aactcagcac
      481 gacgtgcgag gaactcggca agggcctctt tccaatgggg taacggaggg gcaccaaaaa
      541 agcgattgta tttggaagtg tccaatacac tgaagcgcgg tcgcggggcc ttggcaccgt
      601 actcctcggt gctgatcggt gtaatgtcaa cgtccatctt cagttgacgg aagatctcac
      661 gggcgaagcg ataccacgtc gttgccccat cattgacgat atggaacgtc ccatacatat
      721 cggtttggat gaggaaaatg atagcccggg caacgtggac cacgtaactt ggggtacaga
      781 attgatcatc caccactcgc aggggacggc cttcgttcgc caagcgaatc attgtttcca
      841 caaaattgcc cgccgaatgt ggccccaatt ggccatagag accgcacgtc cgcacgatgt
      901 agtgcttttc ccaaacaagg gtttttagct ccccttcgta cttacttcga gcatagacac
      961 cctggggaga gggtacgtcc tcttcacgaa acggctcact ccgttcgggc tcgcccccaa
     1021 agacataatc ggtgctcaca tagagaaagg gacagtccaa ctcccttgcc agatcgacca
     1081 aaacccccgt ggctgtggcg ttgatggcaa aacacttttc gggctcatct tcggccttat
     1141 ccacagccgt ataggccgcc gcgtggatca aaagctctgg tcgcagttcc cggatcgtct
     1201 tacgaacacg atcgtaatcg aggagatcga actcgggcaa atcgagcccc acggcccggt
     1261 cgcccaattg acggcacaac tctcctccca attgtccttt gacgccggtc actgcgattg
     1321 gcataacatc ccctacaaac tacctagtcc tcacagtcgc ttctgtcctc attcccgctt
     1381 ctactagtaa caatctcgcc ggtaagaggc tattcatgct agtataaatg ggaaggcgag
     1441 aaaggaaaaa cgcgattttt gacaaacggg ggtgttaatc caaatcggga aggccgctag
     1501 aacggaggtg agcaccatgc cgatttacga gttctactgt gacaagtgtc acgttattta
     1561 caactttttt tcacgccgga tcaataccga caaacgtccg gactgtccca aatgcggtcg
     1621 ccctaaattg gagcgaaggg tgtcgcggtt cgccattgga cgcgggggca gctcggagag
     1681 taaatcgggc gacaatgccg aagccgatga cgacttgtta ccgccgggct ttgacgaaga
     1741 aaaattcgaa gaggccatga tggaattggc caccgaggcg gaaaacctgg acgaagacga
     1801 tccgcgtcag atggcccgcc tgatgcgacg cctttcccaa aagacgggtc tccaattcgg
     1861 tagtgccatg gaagaagcta tccgcaggct cgaagctggg gaagacccgg agacaatcga
     1921 ggaagagctg ggcgatgtcc ttgagcaaga agagcctctt ttccaacaaa gcggtaaatc
     1981 gctccgcacc ctttacgaac accttaaacc accccgcgtg gacgaaaaac tctacgactt
     2041 ggactaatca acacgcccca ctggtttcgc ggagagcaac aaagcgagaa aaaattgcgg
     2101 tccgtaaatc gatttggaga atggccgact catgggaggg acggcgtttc gccgtccgga
     2161 tgttgaccgc gaataggggc ccattcagaa cccgtagggg caggcccccg tgcctgcccg
     2221 agagcgtcgg gaatgggcct ggatgtcggc cgatgtctgg gtcatcgagg acgatgaccc
     2281 caactcccgg ggcggattgg cacgggacgc ggtcccccgc gtccgtcatt ccggcggaac
     2341 ccctcggccc gatcagacaa aagaaatcgc tccacctcac gcgcaccccc atccctaggc
     2401 acaatcaggg cctatcccct gcccaaaaag tcaagccctg ggcctttttc taggttaaca
     2461 acgcccctac acggcttcaa tcagcttgcg ggcgcggtga agtgcgatag atcaaagtgg
     2521 caggctagcc ggagcggaga atgcctgcgt gtcttcactc gagggcgtta aatcttgatc
     2581 tcatagcctt tccgacttgg gcgtgagagg aaggaattgg cttgcggatc gcccacaatt
     2641 tcgcgcttga cgggatccca ccgcagctct cggcccagtc gcatggcgat gttggccaag
     2701 tggcagattt ccagcatgcg attgtgcgac cacacatcgg agatgggttg tttgcgggcc
     2761 cgcatggcct cgatgaaatt ggcgctgtgg ttggcgctaa tcggtccgcc gtaaatttgc
     2821 tccaaggcat cctcgggcaa ggggttatct tggagctctt cgacgggctt accggtaatg
     2881 cggccacgat tcacaaagaa acggccctca gtcccctcaa agaggatgcc gttgtcgcct
     2941 tcgcttgtga tgatcaattc gatgtcgccg ggcatttcgg cccgaatgtt aaattgcgtg
     3001 gctacattgt aacgatcgtg cacgacggga tagccgtcct tgtactcgca ggccaaggtg
     3061 tagttcagcg gagtaacctt gctggggccg gtgtcggtcg cgccgatggc ccagacggcg
     3121 atgtccacat gatgcgcgcc ccagtcggtc agcttaccgc ccgagtactc gtgccaattg
     3181 cggaatgagt agtggcagtt gctgtagtag ggcacgccac cgccgtaacc ggtgcgcagt
     3241 tcgggcagcg cgcgataggg cactttctcg gcaggtccga gccagaagtc ccaatccaga
     3301 ccttcgggca caggtgcctc ggggatgggc ggcgagggct ccataccgtt gatgccgcag
     3361 gtgacttttc ggactttgcc gatccgtccg tgctttacga gcgccacggc gatcaggaac
     3421 cgcttgtcgc actcggttcg ctgcatggtg ccgacctgga agacgcggcc ggtctctttc
     3481 acaacctttt cgatcagctt cccttcgtcg atcgttaagg tcaacggttt ctcgcaatag
     3541 acgtccttcc cagcgtacat cgcctccacg gcgatcttgg tgtgccagtg gtcgggcgtg
     3601 gcaatcatca ccgcgtcgat gtccttgcgg tcgagcacgt agcgatagtc tttgtaagca
     3661 tccggttttt taccctgccg ttgctctgtc ttttgcacgt tttcggcgag caccttggca
     3721 tcgacgtccg ccaaggccgc gaagtcggca aacttgaagg tcttctgagt gatttcccag
     3781 ccttgattac gcagtccgat tgtggcgaag acgggccgct cgttcgcgga ctcgaacccc
     3841 accgcataac gacgctgcga aagaataatc gctgtcactc ccagtccgac gcttccctgc
     3901 aaaaaacgac gacgtgttgt gcgacggctg atcgacataa gctaaatccc tttcgaaatg
     3961 atgcgatgtt acctgtcggg acatcccgaa cagttgatgc aagacaacgg aaacgcgaac
     4021 ctatctgtgg gtgcggtggg gacgatagac ccaaccctgc atagtgcgct gacgcacccg
     4081 cgattaacgt aagtgtagca aaaactcgga acaaattcat ggaggcgatt tgggcaggca
     4141 cgggggtgga aatttggggc aggcacggcg gcctgcccct acaggttctc aatgggctct
     4201 tattcgtcct ggtcaacggg tatatcgacc cggacggcga gggcgccgtc cctcccaatt
     4261 tcgcgatggg gggtcgccgt ccccggcgac caaatcactg gacaacaccg atccccgttg
     4321 gagacaagcg ctcaacaccg tgacgcacaa tgacacgggc aagatgcccg tgccacgtga
     4381 tttcaatcac ccttctgctt gggaatggca tcaatcgtac gggcaacccc ctgtggttgc
     4441 ccccggaatc tgcgcggacg ctgggcaaat acgaatagtt gttttcgtga atgaaccagg
     4501 cgataatctt attgacttgc cgatccatgc agcatgtttt cttcacgtgt gtggtaacac
     4561 cgagaatgta ggttttctgg g
//
LOCUS       DAOPTR010000166         4560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_15343_length_4560_cov_3.444173, whole genome shotgun sequence.
ACCESSION   DAOPTR010000166 DAOPTR010000000
VERSION     DAOPTR010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15343_length_4560_cov_3.444173"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PKI05_04495"
     CDS             <1..796
                     /locus_tag="PKI05_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOA51490.1"
                     /translation="TGKNAVITGGSSGLGLAIARAFARRGARVAIIGLEQHGVEQARS
                     ELEKHTQVLPLVADIRNEVDVIRCRDAIHSAWRTVDVLVNAAGRSMRGAICDTTPEQF
                     RELFELNVVALVQVTRALLPDLLATRGHVVNIGSLASKAAAKYLGAYPATKHAVAAFS
                     QQLRLELGAKGLHVLLVCPGPIARTGPRLYPLEGEERVLEAARAPGGGVKVKAIDPDR
                     LANKIVKACEKRLVELVVPGRARILFALAQLFPRIGDWLILRFTPH"
     gene            977..2290
                     /gene="xylA"
                     /locus_tag="PKI05_04500"
     CDS             977..2290
                     /gene="xylA"
                     /locus_tag="PKI05_04500"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HOA51491.1"
                     /translation="MAAFPEVSKIRYEGPDSRNPLAFRHYNPDELVEGKPMKDHFRFS
                     VAFWHTMRGTGADPFGAATMIRPWDNGQETVENAINRVRAFFEFCEKLGAPFFCFHDR
                     DVAPEGKTLAETNKNLDAVVKVIKEECDRTGIRLLWGTANLFSNPRYVHGAATSCNAD
                     AFAYAAAQVKKALEVTKELGGAGYVFWGGREGYQSFWNTDMKRELDHLAKFLHMAVDY
                     AKEIGFTGQFYIEPKPKEPTKHQYDFDAAACINFLRTYGLEKYFKLNIETNHATLAMH
                     NVEHELEYAGMQGFLGSVDANTGDLLLGWDTDQFPTDVYLTTKIMLVFMKYGGIAPGG
                     INFDAKVRRESFEPIDLFYAHIGGMDCFAKGLKIAAAIRADGELARMVQERYSSWDTG
                     IGAEIEADKHNFKTLEKYMLEKGDVTPNKSGRQELFENIVNRYIF"
     gene            2512..3123
                     /locus_tag="PKI05_04505"
     CDS             2512..3123
                     /locus_tag="PKI05_04505"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOA51492.1"
                     /translation="MKKLNVLVIWGLILGLCVLSTSRGMVAAEEAAAKEEEPKFIRTP
                     DVIYVPTPYDVVRKMLELAEVKKGDVVYDLGCGDGRIVVTAAKEYGVKAVGFDIDPER
                     VKEARENVKKNKVEHLVRIEQKDIFTLDLSEATVVTLYLLPQLNVKLIPQLEKLKPGS
                     RIVSHDFDMAGVIPDKVVNFKSKNGREHTLYLWTTPLKKEKTQ"
     gene            complement(3146..4324)
                     /locus_tag="PKI05_04510"
     CDS             complement(3146..4324)
                     /locus_tag="PKI05_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51493.1"
                     /translation="MKLASIRRFSVLVTGLVLLGCSTNKTGSNFNLARVNPFHWIKPE
                     SETKPYPQKPSSFASPSDATGRYSGTVADSTKGGTLPAAASAQVYPGTQAGYQNVEST
                     TYPGTASMRSGTPYGTTAQSYPATTTTPPSGGYSANPVTATSWQSNPAGVATASDQRN
                     FSQPASSGNTGSMPANPYVPSYSRTTQSATLPSAESSSQLSGSGTNYRPPAPTSEAKM
                     SPSALQGAATGGTTDWTTLVGERYAQLYQKGSNMPSPSGTDGLGSTGYSPGDTGYVPG
                     KLENPPGNVNYRPGQTGYNPPGVPPYAMPYGTNSQQTNASDRSWPTSTNSAGEYRPGS
                     TKTYIPRTGSQSSNTTVPGTGTNQAATGGLSSFPTTGSLSDQPSNISQTSSVNEPLRM
                     "
     gene            complement(4362..>4560)
                     /locus_tag="PKI05_04515"
     CDS             complement(4362..>4560)
                     /locus_tag="PKI05_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51494.1"
                     /translation="HENKFCGKKSPVSAFPECFLRARHAESVRNFRHPPGSLGAVIKH
                     EGSIGWIGFFTRIPAKTIRAR"
BASE COUNT         1035 a         1133 c         1329 g         1063 t
ORIGIN      
        1 gactggcaag aatgcagtca ttacaggagg ctcgagcggg ttggggttgg caatcgcgcg
       61 ggcatttgcc cgacgtggcg cgagggtagc cattatcggc ctcgaacaac acggcgtgga
      121 gcaagcacga agcgaattgg aaaaacacac gcaagtcctg ccgcttgttg cagacatacg
      181 gaacgaggtc gatgtcattc gctgccgcga tgccatccat tcggcctggc gaacggtcga
      241 tgtattggta aatgctgccg ggcgttcgat gcggggcgca atttgcgata caacaccgga
      301 acagtttcgc gaactctttg agttgaacgt tgtagccctt gtgcaagtaa cacgcgcctt
      361 gttgcccgat cttttggcta cccgcgggca cgtcgttaat atagggtccc tggcatccaa
      421 agcagcggcg aaatatttgg gtgcctatcc cgcaacaaaa cacgccgttg cggcgttcag
      481 tcaacaactt cgcctggagt tgggggcaaa agggctgcac gtcttgctag tctgtcccgg
      541 tcccatcgcg cggactggcc caagactcta tcccctggag ggtgaagaac gagtcctcga
      601 ggccgcccgg gcgccaggtg gtggcgtcaa agttaaggca attgatcctg acagacttgc
      661 caataaaatc gtcaaggctt gtgaaaagcg attggtcgag ctggttgttc cgggtcgcgc
      721 gcggattctt tttgcattag cccaactatt tccccggata ggcgattggc tcattcttcg
      781 atttacccca cattaagtga gattctcatg cgaccagcga gtctttctct cgatggaaaa
      841 cgtctcgaac acccctcttg cgatcgatgc cgcaatgtgt caccatagta ggccacactg
      901 agtgtgtgat taaagaactc cggcaagagg aagaagttgc gtcaagaaaa agccgtgtca
      961 tcaaggagat tgaaccatgg ctgcttttcc cgaagtgtcg aagattcgtt atgaaggtcc
     1021 agactctcgg aacccgttgg ctttccgaca ctacaatccg gatgaacttg tggaaggaaa
     1081 gccaatgaaa gaccatttcc gctttagtgt ggccttttgg cacacaatgc ggggaacagg
     1141 ggccgatcct tttggagcag caacgatgat tcgcccctgg gacaatggcc aggagaccgt
     1201 cgaaaatgcc atcaaccgcg tccgggcgtt ctttgaattc tgcgagaagt tgggggcccc
     1261 gttcttttgt ttccatgatc gcgacgtagc gccagaagga aagacgctcg ccgagacgaa
     1321 caagaatttg gatgcagtgg ttaaggtcat caaagaagaa tgcgaccgca cgggcatccg
     1381 tttgctttgg gggacagcca acctcttcag caacccccgg tatgttcatg gagctgccac
     1441 cagttgcaac gccgatgctt ttgcctacgc cgcggcacag gtgaaaaagg ccttggaagt
     1501 taccaaggag ttgggtggcg ccggttatgt attctggggt ggtcgagaag ggtatcagag
     1561 tttctggaac accgacatga aacgggaact cgatcacctg gccaaattcc tccacatggc
     1621 agtggactac gccaaggaga tcggttttac cggccagttc tacatcgagc cgaaacccaa
     1681 ggaaccgacg aagcatcagt atgatttcga tgcggcggcg tgcatcaatt tcctgcggac
     1741 ctacgggctg gaaaagtact tcaagctcaa catcgagacg aaccacgcaa cattggccat
     1801 gcacaacgtt gagcacgagc ttgaatacgc cggcatgcaa gggtttctcg ggtctgtgga
     1861 tgccaacacg ggtgatttgc tcctcggttg ggacaccgat caatttccca ccgatgttta
     1921 cctgacaacc aaaattatgt tggtcttcat gaagtatggt gggattgcac cgggcggaat
     1981 caacttcgac gctaaggtcc gtcgagaaag cttcgagccc attgatcttt tctacgccca
     2041 catcggagga atggactgct ttgccaaggg tctcaagatt gcggcggcaa tccgggccga
     2101 tggcgagctc gcccgcatgg tccaagaacg atacagctcg tgggataccg gcattggggc
     2161 tgaaatcgag gccgacaaac acaacttcaa gaccctcgag aaatacatgc tagaaaaggg
     2221 tgacgtgacg cccaataaga gtggccgtca agaattgttt gagaacatcg tgaatcgtta
     2281 tatcttctga cttgcgtgat cggtgttccg atcgtgtgag gtgttgtttc cagaccggtc
     2341 cagagaggcc ggtctttttg ttttccaaac agcggaacct ccctcactgc gtgggacatt
     2401 cctggcctcc ttgatctgct ttacaaccac cttaagcgag gttatcttat ctgtcagtcc
     2461 gtgggtttta cgaccgtgat acgtcgtttt tcggagaaag gagtgagtgc aatgaagaag
     2521 ctaaatgttt tggtcatctg gggattgatc cttggtttgt gtgtcctcag cacttcgaga
     2581 ggaatggtcg cagccgaaga ggcagcggca aaagaggagg aaccgaagtt tatccgcaca
     2641 ccagatgtga tctatgttcc gacgccgtac gatgtcgtcc gcaagatgtt ggaattggcg
     2701 gaggtgaaaa agggcgacgt agtttatgac ctgggctgcg gtgatgggag gattgttgtc
     2761 accgcggcaa aggaatacgg tgtcaaagcg gttggttttg atattgatcc cgagcgggtc
     2821 aaagaggcca gggaaaatgt gaaaaagaac aaggtcgaac acctcgtccg gatcgagcaa
     2881 aaagatattt tcacactgga tctgagcgag gccacggttg tgaccttgta ccttctgccg
     2941 caactcaacg ttaagttgat ccctcaattg gaaaagctca agccgggttc tcggattgtg
     3001 tcacacgact tcgatatggc cggggtcatt cccgacaagg tcgtgaattt caaatccaag
     3061 aatggtcgtg agcatacgct ctacctctgg acaacaccgc tgaaaaaaga gaagacccaa
     3121 taacctcagc ggcgtttcgg cccaattaca ttcgcagtgg ctcatttacg ctactggttt
     3181 ggcttatgtt cgagggttga tctgataagc tgccagtagt gggaaacgaa gacaagccgc
     3241 cggttgccgc ctggttcgtc cctgtccccg ggacagtcgt attgctcgac tgactaccgg
     3301 ttcgcgggat gtaagtcttc gtgcttccgg gccggtattc gccagcggag ttggtactgg
     3361 taggccaact tctatccgat gcgtttgtct gctggctatt cgtgccgtaa ggcatagcgt
     3421 atggcggaac tcccggagga ttgtagccgg tttgacccgg ccggtaattg acatttccgg
     3481 gcgggttttc tagcttgccc gggacgtatc ccgtgtcgcc gggcgaatag cccgtacttc
     3541 caaggccgtc ggtccctgac ggcgatggca tgttactccc tttttggtag agttgagcgt
     3601 accgctcgcc aaccagcgtg gtccagtctg tggtaccccc ggtggctgct ccttgcaaag
     3661 ccgacggtga catctttgct tcggaagtcg gtgcaggtgg acgatagttc gttcccgatc
     3721 cacttagttg ggatgatgat tcggcagagg ggagagtcgc actctgtgtc gttcgggagt
     3781 aactgggaac atacggattt gcgggcattg aacccgtgtt gcctgaggac gcaggctgag
     3841 aaaagttcct ttgatcactg gccgtggcga ctcctgccgg gttggactgc caggacgtgg
     3901 cggtaacagg atttgccgaa taaccaccgg agggcggtgt ggtggtggtt gcaggatatg
     3961 attgtgctgt ggtgccataa ggtgttccag aacgcatgga tgctgtaccc ggataagtgg
     4021 tggattcaac attttggtac ccagcctgcg taccgggata cacttgggcg cttgcggctg
     4081 ccggcagtgt tccacctttg gtcgagtcgg caacggtgcc ggaatatcgc cctgtggcat
     4141 cgctcggcga ggcgaaagaa gacggttttt gcgggtatgg cttggtttcc gattcgggct
     4201 tgatccagtg gaaggggtta actctcgcga gattgaaatt ggaaccggtc ttgttcgttg
     4261 agcaacctag caggacaagg ccagtcacca acacagaaaa ccgtcggatt gaggcgagct
     4321 tcatttctgg gccatccttg gctaaacagt tccctgggat attagcgggc acgaatggtc
     4381 ttcgccggaa tgcgagtgaa aaaccctatc caaccaatac tgccctcgtg cttgatcact
     4441 gcccccaggc tccctggagg atgccgaaag tttcgcacag actcggcatg acgtgctctc
     4501 aaaaaacatt ccgggaaggc ggacacgggc gattttttac cgcaaaactt gttttcgtgc
//
LOCUS       DAOPTR010000167         4559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_15346_length_4559_cov_3.568162, whole genome shotgun sequence.
ACCESSION   DAOPTR010000167 DAOPTR010000000
VERSION     DAOPTR010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15346_length_4559_cov_3.568162"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PKI05_04520"
     CDS             <1..231
                     /locus_tag="PKI05_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliP"
                     /protein_id="HOA51495.1"
                     /translation="PAFMLSELKTAFLIGFQIYLPFLIIDMVVSTIMVSMGMLMMPPV
                     LVSLPFKILLFVLLDGWHLVVGMLMESFQPFV"
     gene            254..520
                     /gene="fliQ"
                     /locus_tag="PKI05_04525"
     CDS             254..520
                     /gene="fliQ"
                     /locus_tag="PKI05_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HOA51496.1"
                     /translation="MEPQAAVDLFRECAWLALLISAPILLAGMAVGLAIGLLQAITQI
                     QEQSLAFVPKVAVMVLVFLLTLPWVLIQLTEYSQELIRHIPEMF"
     gene            566..1339
                     /gene="fliR"
                     /locus_tag="PKI05_04530"
     CDS             566..1339
                     /gene="fliR"
                     /locus_tag="PKI05_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HOA51497.1"
                     /translation="MPIPLILEKHLMIFIFVFVRLGGMMAVAPLFSRKDIPAQVRVFL
                     AVALALLLTPTQLDTALPNPQGVIEFALLAGGEFFVGIVLGLGLMILIQGMGLAGELI
                     SRISGIGMNDVYDPTSETEVTGMSQFIMTVGLVTFLCFSGHRALIAGLLESYRELPIG
                     KTFQSQEVVSLLMAMLQSSFILAIRVAAPAMAALLVVTLVLGLISRTIPQVNLLMLGF
                     GLNGLAAFGLVLLTVGITTGVFEHELVSALDQLFPWGFE"
     gene            1352..2431
                     /gene="flhB"
                     /locus_tag="PKI05_04535"
     CDS             1352..2431
                     /gene="flhB"
                     /locus_tag="PKI05_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HOA51498.1"
                     /translation="MPDQAGERTIEPTQHRRQKAREEGHVAHSADLVSAIVLLSGTLA
                     LFFFGRELSDFFFQLTKQFLGEVLITSDSGVVTALLTTVAQKLAIILGPILLAIIAAS
                     IAAHFVQFGFLFLPQKLAPDITRIDPLKGFQKIFSLANLVRLGFGILKMLAVGTVAAV
                     YLLTQFDTILGLSSLVPVEIAAYIVELLIWTTIRLGIALLVLAILDFGFQYWKREQDL
                     KMTPQEFREEMRNLEGDPQIAARRKVVQRQLMLNRVNQAVPKADVVVTNPTELAVALQ
                     YIPEKMAAPIVVAKGAGLVAQRIRRLAEQHGVPVIEKKPLAQALYRTVEVNQPIPQEL
                     YAAVAEVLAYVYHLKGKKPSIPKAA"
     gene            complement(2473..3678)
                     /locus_tag="PKI05_04540"
     CDS             complement(2473..3678)
                     /locus_tag="PKI05_04540"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HOA51499.1"
                     /translation="MTKNGVKTKEVGGYRDRPWIPRIWDGITFLGWVRVMAHHRFRIA
                     PRRLGMAILITFLGLFNSFLALIQQAILGRKINRTKLPGDPIFIIGHWRSGTTLLHEM
                     FILDPRWGFPDSYACFAPNHFLISRCFLAPLLGLLMPKMRPIDNMPFDWNRPQEDEFA
                     LCNMGLPSPYLSLIFPNEKPRYDAYLTLEGLTEKQLSRWKRTFLWFLKAVTVRNPRPM
                     VLKSPPHTCRIRVLLEMFPQAKFVHIYRDPYAVFPSTVNLWKRLSTDEALQIPHHQGL
                     EERVFDIFCKMYEVFERDRELIPPHQFAEVSYEALVADPEEEMERLYRELDLGDFEIL
                     RPRLRQFIAERSNYKRNRFELAPALKNEIARRWGFFFQKYGYSIEQTEAAESVPSANV
                     TSPQKNPLG"
     gene            4297..>4559
                     /locus_tag="PKI05_04545"
     CDS             4297..>4559
                     /locus_tag="PKI05_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase family
                     protein"
                     /protein_id="HOA51500.1"
                     /translation="MNVLVIGSGGREHALAWKLKQSSLVDRVFVAPGNAGTAEDAENV
                     PIPETDFAQLIKFVKENGVDLTVVGPEALLAAGIVDAFRKEKLR"
BASE COUNT         1002 a         1187 c         1228 g         1142 t
ORIGIN      
        1 ccggcgttta tgcttagcga gctcaagacg gcttttctta tcggatttca gatatatctg
       61 ccatttctga tcattgacat ggtagtatcc acaatcatgg tctcgatggg catgctcatg
      121 atgccacccg tgttggtctc actgcccttc aaaatacttt tgttcgtcct gcttgatgga
      181 tggcatctcg tggtcgggat gcttatggag agttttcaac cgtttgtgtg aaagggaaac
      241 atcgccgaga gccatggaac cacaggccgc cgtggatctg tttcgtgagt gtgcttggct
      301 ggcgctgcta atcagcgctc caattttgct cgccggaatg gccgttgggc ttgcgatcgg
      361 tttgttgcaa gcgatcacgc agattcagga acagagtctc gccttcgtac cgaaggtggc
      421 cgttatggtc ttagtctttt tattaacgct cccctgggtc cttattcaac ttactgaata
      481 ttcccaggag ttgatccgac atatccccga aatgttttga tagtaccttc cttagtggac
      541 tcttgcagcc atcaaaacgg aaactgtgcc aatcccattg atccttgaaa aacacctgat
      601 gatattcatc ttcgtcttcg tccgattggg cgggatgatg gcggtggcac ctttgttttc
      661 gagaaaggac attccggctc aggtcagagt ctttctggcc gtggccctgg ctttattgct
      721 gacgcccacc caactggata cggcgctccc caatccccaa ggagtgatcg agttcgcgct
      781 ccttgccggg ggcgaatttt ttgtcggaat cgtcctagga ttggggttga tgatccttat
      841 tcaggggatg ggactggcag gcgaattgat ctcgcgaatc agcgggatcg gaatgaatga
      901 cgtctatgat cccacaagcg agaccgaagt caccgggatg tctcaattca ttatgaccgt
      961 agggcttgtg acctttctct gtttttccgg ccaccgggcg ctgatcgccg gccttctaga
     1021 aagctatcgc gaactgccca tcggcaagac gtttcaatcg caggaggtcg tgtctctgct
     1081 gatggccatg ctgcaatcca gctttatctt ggccatccgt gtggcggccc cggccatggc
     1141 ggcccttttg gtggtgaccc ttgtcttggg cttaatcagt cgcaccatcc cccaggtcaa
     1201 tctcttgatg ctggggtttg gattgaacgg cttggcggcc ttcggcctcg tcctactcac
     1261 ggtcggtatc accacaggtg tttttgagca tgagctggtc tcagcacttg accagctttt
     1321 tccttggggc tttgaatagt cggttgcgac catgccggat caggcgggcg aacgcacaat
     1381 tgaacccacc cagcatcgcc ggcaaaaagc ccgcgaggaa ggacacgttg cccatagtgc
     1441 cgatcttgtc tcggcaattg ttctcctgtc gggcaccctg gctcttttct tctttggccg
     1501 cgagctgagc gatttctttt ttcaactgac aaagcaattc cttggcgagg tgctgattac
     1561 aagcgattct ggggtggtca ccgctttgtt aaccactgtt gctcaaaaac tggcaatcat
     1621 ccttggcccg atcttgctgg ccatcatagc ggcttcaatt gcagcccatt ttgttcagtt
     1681 tggattccta ttcctccccc agaaacttgc cccggacatt acgcgaatcg atccgttgaa
     1741 gggatttcaa aaaatcttct cactggcaaa tcttgtccgt ctaggcttcg gtattttgaa
     1801 aatgctggcg gtgggaactg tcgccgccgt gtatttgttg acacaatttg acacaatcct
     1861 cgggttaagt tcccttgtcc ccgttgagat cgcagcctac atcgtagaac tcctcatttg
     1921 gacaacgatt cgtcttggta ttgccttgct tgtactggcc attttggatt tcggcttcca
     1981 gtactggaaa cgcgaacaag acctgaagat gacgccgcag gaattccgag aggaaatgcg
     2041 gaatctagaa ggtgatcccc aaattgcggc gcggcgaaaa gtcgtgcaaa ggcagcttat
     2101 gttgaatcgg gtcaatcagg ccgtacccaa ggccgatgtt gttgtcacca accccacgga
     2161 gttggccgtg gcgctacaat atatcccgga gaaaatggcg gcaccgatcg tagttgctaa
     2221 aggagccggt ttagtagccc aaaggatccg tcgcctcgcg gaacagcacg gtgtgcctgt
     2281 cattgaaaag aaacccctcg ctcaggccct ttatcgaacg gtggaagtca accagccgat
     2341 cccgcaggaa ctgtacgcag ccgttgcaga agtccttgcc tacgtctatc acttgaaagg
     2401 caaaaagccc tctatcccca aagcggcata atcactgaca tgaggtcgcc tcctgtccgc
     2461 gaccagagtt gattagccca ggggattttt ctgcggagac gtcacattgg cggagggcac
     2521 tgattcggcc gcttctgttt gctcgatgga gtatccatat ttctggaaga agaatcccca
     2581 ccgccgcgcg atttcattct tcagagcggg cgccagctca aagcgattac gcttgtaatt
     2641 ggatctttcg gcaatgaact gcctcaaacg aggacgaagg atttcgaagt cacctaggtc
     2701 taactcccga tagaggcgtt ccatttcttc ctcgggatcc gccacaaggg cctcgtaact
     2761 gacctccgcg aactggtgcg gggggatgag ttctcgatcc ctttcgaaga cttcatacat
     2821 cttacaaaag atgtcgaaga cccgttcctc caagccctga tgatgcggaa tctgaagggc
     2881 ctcgtccgtt gagagtcgct tccacagatt gaccgtcgaa gggaatacgg cgtacggatc
     2941 gcgatagatg tgaacaaact tcgcttgcgg aaacatctcc agaagcacgc ggattcggca
     3001 ggtgtgcggc ggagatttga ggaccatagg ccgcggattc cgtacggtca cggccttaag
     3061 aaaccatagg aatgttcgct tccatcgact cagttgtttc tccgtgagcc cctctagcgt
     3121 caagtaggca tcgtaacgtg gtttttcgtt gggaaagatc agactcaaat agggtgaagg
     3181 caaacccatg ttgcagaggg caaattcgtc ttcctgcggt ctgttccaat cgaacggcat
     3241 gttatcgatc ggccgcatct tgggcatcaa aagcccgaga agcggagcca ggaaacatcg
     3301 cgagatcaaa aaatggtttg gcgcgaaaca ggcataactg tccggaaagc cccaccgagg
     3361 atcgagaatg aacatctcgt ggagaagcgt ggttcccgat cgccagtgac caatgatgaa
     3421 gatggggtcg ccgggcagtt tagtccggtt aatctttctt cccaggattg cttgctgaat
     3481 caaggccaaa aacgagttga acagcccaag aaatgtaatc aggatcgcca ttcctaggcg
     3541 acgcggggcg attcggaagc ggtggtgggc catgacccta acccacccaa ggaaggtaat
     3601 gccatcccag atgcgtggga tccatggtcg gtcgcggtag cccccaactt cctttgtttt
     3661 gactccgttt tttgtcatca cactattcca tcgcgaggga ggttttcgcg caacttgtgc
     3721 agaagccccc agagaacttt aatgcgggac ttttatgggt gaggtttgac atgatctgag
     3781 aaagctacct gagttgccca aaacgattat tttactctat tgtccccgca tttcctacgg
     3841 agagctttcg tagactttgc cgaaatcata aagtttattc gggcatttcc gtgctaaatc
     3901 ctcaattgga tttgtctcga gcggtcacca gcggaaaaag gcaagcgccc gtaggaccgc
     3961 cgggccaatc cgaaactccg ccgaacgcct ggtttgttag aaagctgaag cactaagagc
     4021 ctatgacatc atctcccctc gcccgctggc cgggagaggg gtcgggggaa gggtagctcg
     4081 aatcgctgcc ggtttcctcg atcaactccg acgttcgctt gccctcaccc caaccctctc
     4141 ccggagggag aggggggact ttttgggaag gctctaaaca gcgtgctagg tgacctgcct
     4201 cacacaggca tcctttccag gcttagctgc attccctaga atcagcattt ggtcacttgt
     4261 gagagcttga gataggctat ttggagttgt cggtcaatga acgtcttggt gatcggaagc
     4321 ggtggacgtg agcacgccct tgcgtggaaa cttaagcaaa gctcattggt ggatcgggtg
     4381 tttgtcgcgc ctggcaatgc cggaacggcg gaagatgctg agaatgtccc gattccggaa
     4441 accgatttcg cgcagttgat caagttcgtt aaggaaaacg gtgttgacct gacggttgtc
     4501 ggcccggaag cgctgttagc ggccggaatc gtggacgcct tccggaagga aaaactccg
//
LOCUS       DAOPTR010000168         4551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_15385_length_4551_cov_2.902580, whole genome shotgun sequence.
ACCESSION   DAOPTR010000168 DAOPTR010000000
VERSION     DAOPTR010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15385_length_4551_cov_2.902580"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2416)
                     /locus_tag="PKI05_04550"
     CDS             complement(<1..2416)
                     /locus_tag="PKI05_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51501.1"
                     /translation="MRRLLFAVHQYFRIVVLAWLCPVVIGMSATAGADDRPIDWSSWK
                     RLPLFEGGRIMPLDTFARRIVEKLTGKPQPILGPPMTPDGQLDRQHPLFPDGLLRKWP
                     AVELLFSWLAESEKWEDVPFLQASHQDLRERLLKVPLVDASGKRLSYVSPRDVENSME
                     FEAYLRDLSQRRREVESRGQRFRMSGLDLHAARLWDAYATFRLLTYQPRLDSRPWFPP
                     NVKVSHPIPRRLESLIDSWTEIEPQLQQLADLGVFSSGQSQTAGPGEPGEAPSGDQSG
                     SQEHLVARARAEFAPLLRAAESEEVTVAELDEALTRLQPLLREIALCVESLQNRAFSV
                     EAPDATKQLRLDDLRSNIRLLAEKSRRLADEVEVCVPMLYDTGVGPHVLPAMIPACLN
                     SATNGDEPVSPWLSLGAILYGSKNVLRGYDIDRVNQVRSFFQMAAAAYADRRSHDRTR
                     RVEENLDQFSAALAKLGRSIEAERERLPVDESHRELLALTAYPPAGFTDREVFYNEFD
                     PFRWFWIINLLAFGILLLGVGRLRQPSLWIGVLVLTVAQVVTIYGFGLRVAISGWAPV
                     TNMFESVVFTSLVIGLLGVWFVIRPLVGKGIREGWEWVSLPHEPLTGKREARTWQDKG
                     TFVNVLRGAGVLARLALGGLLFYYLAVVPYDVGKSRAVFDLVPDITRSLGSPTFSSIV
                     MNLLGWFVGMCVLLSVVWLVPRFAVGMILSLLWTIPVDLKTAELGPRLNEVVLRRPFA
                     VAAAGLAFLAAVVAYYTPVWDKGIEPLQPVLRDRLWLFAHVLTITAGYGAGLLAWGLG
                     NISLLYF"
     gene            complement(2621..>4551)
                     /locus_tag="PKI05_04555"
     CDS             complement(2621..>4551)
                     /locus_tag="PKI05_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51502.1"
                     /translation="LAGAYWTALEGIDAQLSIYEGMIGHIAYQDTVHFEIDLTKKSRS
                     PNQDNPSAGSTENAADSRPSERRALRVAVPFHCGPFNWCDYSSGLRHLLWSPPGRLPV
                     LPWQLAWRDKGVIFADGDLSLEVLDYYADSRRVRGGYLAGYIKANTATSAQGTWFRPW
                     EPIELDVQSRRTEAGITTAIGSRVTLSNGIRVVLTAARSQAELDGFLLGTPVEPLDHE
                     GQVVLVHNQIRYVLPVRQLRAATGDTPLEGSGLRITHFEKDVPLQAVQLQLKNDEGVE
                     DEVILFADLPEFNRQGQKLAVAGAYWVNFPSLLSDVGQAVRLHPRATTGATRARVDLC
                     QGPGGKLYYRIWQSPQVLKTGEINRDDRESILLVGNDLDVRLVVNTFVPWDPEHPEAE
                     TVVPLPFRKGARMTQPRVKVRLSAGGKTHETWLAVLPRGADRPIDAEEIATVASDTEQ
                     MRVTVKYDAVDVGFRLKLREFQRKLDPGTNQPSYYASLVDLYVPERIAKEHGFENPRW
                     KDQTIIDPSDGEPLQLVHPRILITLNHPANIVDPITGRSYRLYQESFSGPFRPGDPIY
                     EQVVGKEKGPPELYLSTLSVNYDPGRGLKYAGSLLIIGGIVLMYLMRVYIFRPRVRPP
                     FRNEQLEQSVAAGPVSVT"
BASE COUNT         1122 a         1362 c         1145 g          922 t
ORIGIN      
        1 ggaaataaag caacgagatg tttcccaacc cccatgcgag taacccagca ccatatcctg
       61 ccgtgatcgt caggacatgg gcaaaaagcc acagccgatc acgaaggacc ggctgcaggg
      121 gctcgatccc cttgtcccac acaggggtat aataggcaac gactgctgcc aaaaaggcca
      181 atccggcggc tgccacagcg aacggtcgcc gaagcaccac ttcgttgagg cgcggtccca
      241 gttctgcggt cttcaagtcc actggaatcg tccagagaag cgacaaaatc attcccacgg
      301 caaagcgagg cacgagccac acgacggaca ggagaacgca cattcccaca aaccacccca
      361 agaggttcat cacgattgag gagaaggtcg gacttcccaa agaccgcgtg atgtccggca
      421 ccaaatcgaa cacggcccga ctcttgccca catcataggg aacgaccgcg agatagtaaa
      481 acaggagccc gcctaaagcc aatcgtgcga ggacgccagc tccccgaagc acgtttacaa
      541 aggtcccctt gtcctgccaa gtcctcgctt cccttttgcc ggtcaacggc tcatgcggca
      601 gagacaccca ctcccagccc tcgcgaatcc ctttgcccac caacggccgg ataacaaacc
      661 acacgccgag cagaccaatc actagcgacg tgaatacaac cgactcaaac atattggtca
      721 caggcgccca gccagagatc gccacgcgaa ggccaaaccc gtagatcgtc accacttggg
      781 ccacagtaag aacaagtacg ccgatccaca aagaaggctg acggagacgt cccacgccaa
      841 ggagcaatat gccaaacgcc aaaagattga tgatccaaaa ccagcgaaat gggtcaaact
      901 cgttgtaaaa tacttcccga tcggtaaacc ctgctggcgg ataagcagtc aaagccaaca
      961 attctcgatg agactcatcc accggcaaac gctcccgttc cgcttcaatg gatcgtccta
     1021 acttagccaa cgctgcggag aactgatcga gattctcttc tacgcggcgc gttcgatcgt
     1081 gcgatcgacg gtcggcatat gccgcggcgg ccatctggaa aaagctgcgg acctggttca
     1141 cccggtcaat gtcataccct cggagcacat tctttgaacc atagagtatg gcgcccaaac
     1201 ttagccacgg ggataccggc tcgtcaccgt tcgttgccga attaagacaa gcggggatca
     1261 tcgccggcaa cacgtggggg ccaacgccgg tatcgtacaa catcggaacg cagacctcga
     1321 cctcgtccgc cagacgccgc gacttttctg ccaggaggcg gatattgctg cggagatcgt
     1381 ccaggcgaag ttgcttggtc gcgtcgggtg cctcgactga aaaggctcga ttttgcagcg
     1441 attccacaca cagggcaatt tcgcggagca gcggttgcaa tcgggtaagc gcctcatcca
     1501 gttccgcaac tgttacctcc tcagactccg cagcgcggag aagcggggca aactcggccc
     1561 tcgcacgggc aacgagatgt tcttggctgc cagactggtc tccagaaggg gcttcccctg
     1621 gctcacctgg ccccgcggtc tgtgactgac cggatgaaaa tacccccaaa tctgcgagct
     1681 gttgaagttg cggctcaatc tctgtccagg aatcgattag agattccaaa cgccgcggaa
     1741 tgggatggct caccttgacg ttaggtggaa accagggacg gctatcaaga cgtggctgat
     1801 atgtcagtag gcgaaatgtg gcatatgcgt cccacaatcg tgccgcgtgg aggtccagac
     1861 cgctcatccg gaagcgttgg cctcgggact caacttccct ccgtcgctgg ctgaggtcac
     1921 ggagataggc ctcgaactcc atggagtttt ccacatcccg tggcgagaca tagctcaggc
     1981 gtttcccgga tgcatcaacc aatggcactt tgagaagccg ctcacgcaaa tcttggtgac
     2041 tcgcttgcag gaacggcacg tcctcccact tttccgattc agccagccaa ctgaaaagca
     2101 gttcgaccgc tggccatttt cgcagaagac catctggaaa caacggatgc tgcctatcca
     2161 attggccatc gggcgtcatc ggaggaccaa gaatcggctg cggctttcca gtcagctttt
     2221 ccacgatacg acgggcaaag gtatcaagcg gcataatacg accaccttcg aagagaggaa
     2281 gtcgcttcca cgaggaccaa tcgatgggcc gatcgtccgc cccagccgtc gccgacattc
     2341 cgattacgac cgggcacaac caagccaaga cgacaatacg aaagtattgg tgaaccgcga
     2401 ataataacct gcgcatttcc atcgtcatca atgaaccgat tcttcgtata ctggcatgga
     2461 caggtccgca atttgcccga cagaaaacca tcttattgcc gctaacaatt caaacagata
     2521 aagaaaatcc ttttcggtga aacacgctcc gctaatttgg taaccttgcg ttgtgggtcg
     2581 aagtatctgg atctgttctt gagtcttact attcatacgt tcacgtcaca ctaaccggcc
     2641 cggcagccac actttgctcg agctgttcat tcctaaacgg tggccttacc cgcgggcgaa
     2701 agatgtaaac cctcatcaaa tacatcagga cgatgccacc gatgatgagc agcgacccgg
     2761 catatttcag ccctcgcccc gggtcatagt taacgctcaa tgtcgagaga taaagctccg
     2821 gcggtccctt ttctttgccc acgacttgtt catagatcgg atcgcccggt cgaaacggac
     2881 cgctaaaact ctcctgataa aggcggtaac tgcgtcccgt aattgggtcc acgatgttcg
     2941 ccggatgatt taatgtgatc aagatcctgg gatgcaccaa ctgcaacggt tcgccatcgc
     3001 ttgggtcgat gattgtttgg tctttccacc ggggattctc aaagccatgt tccttggcaa
     3061 tccgctccgg cacatagagg tcaaccagac tggcgtagta cgacggctga tttgtaccgg
     3121 gatcaagctt gcgctgaaat tcccttagct taagccgaaa gcccacatca accgcatcgt
     3181 atttaacagt cactcgcatt tgctcggtat cactagccac tgtggcaatc tcttcggcat
     3241 cgatgggtcg gtcggcccct cttgggagga ctgccaacca cgtttcgtgc gtctttcccc
     3301 cggcagataa tctcaccttg accctcggtt gggtcatgcg cgcaccctta cgaaacggca
     3361 aaggaacaac cgtctccgcc tccggatgtt ctggatccca tgggacaaat gtgttgacta
     3421 ccaaacgaac atctaaatca tttccgacga gcaggatcga ttctcggtca tcgcggttta
     3481 tttctcctgt tttcaaaacc tgaggcgact gccaaattcg gtaatacaat ttgccaccag
     3541 gaccctgaca aaggtccact cgtgcccgtg tcgcccccgt ggttgcgcgc ggatgaagac
     3601 gtactgcttg tccgacatcg gacagaagac tagggaaatt gacccaatat gcgccggcga
     3661 cagccagctt ttgtccttgt cgattaaact cgggaagatc ggcaaaaagg atcacttcat
     3721 cttcaacacc ttcgtcgttc tttagctgca attgcacggc ctgaaggggc acgtcctttt
     3781 caaagtgagt gatccgtaaa cctgagccct ccaacggggt atcacctgtt gctgcccgca
     3841 attggcgtac aggtaggaca tagcgaatct gattgtgaac caggaccact tgcccctcgt
     3901 gatcaagagg ttcgacgggc gttcccagca ggaaaccgtc aagctcggcc tgagagcgtg
     3961 cggccgtcaa gacaacccga atgccattgc tcagcgtcac ccgcgagcca attgccgtcg
     4021 ttatgccagc ctccgtacgt ctggactgaa catctaactc aataggttcc cagggacgaa
     4081 accacgtacc ttgagccgac gttgcagtat tcgcttttat atagccggcc agataaccgc
     4141 ccctgacacg tcgcgagtca gcgtaataat ccagcacttc caaagataga tcaccgtctg
     4201 caaatatgac tcctttgtcc cgccaggcaa gttgccacgg caagacgggc aatcgtcccg
     4261 gaggcgacca caacaagtgt cgtaaacccg atgaataatc gcaccaatta aaaggcccac
     4321 agtggaaagg caccgctacc cgcaacgccc gtctctcgga aggccttgaa tccgccgcat
     4381 tttcggtgga cccagccgag gggttatcct ggtttgggct ccgcgacttc ttcgtgaggt
     4441 cgatctcgaa atgaacggta tcctgataag cgatatggcc aatcatcccc tcatagatgg
     4501 aaagctgggc gtcgattccc tctagggcgg tccaatacgc accggccagc a
//
LOCUS       DAOPTR010000169         4548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_15397_length_4548_cov_3.327621, whole genome shotgun sequence.
ACCESSION   DAOPTR010000169 DAOPTR010000000
VERSION     DAOPTR010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15397_length_4548_cov_3.327621"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="PKI05_04560"
     CDS             <1..1330
                     /locus_tag="PKI05_04560"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HOA51503.1"
                     /translation="AINRLPGPRWRVHWMLWYWVVMFVVSLAQLGGIAHGVGQALAIA
                     LPIDGSFRQLLKEQEEWDREAAQIRRELEASFEQPKGSGSAGTASVDKKTVETEVAQR
                     MQRPRPGYENREGLLTDDLIWAIIICLVTIPILVYGRYGMIQWVCTILVVGFTLVTIF
                     CVIGIQFKPEWAISWDDLREGLSFRLPGTANRWLALSTALATFGIIGMGTNELITYPY
                     WCLEKGYARFTGPRDNSPGWAKRARGWMRVMRADAFLSMVVYTFVTAAFYLLGAAVLY
                     RSELIPAGSQMISYLSEMYVPVLGEWTKAFFLFGAFAVLYSTFFSATAGHTRVAADAV
                     AIVRRGRSQPDRPPQSWVTAFSIGFPLFSLVTCLFYREPVTLIVASGFMQAIMLPMLA
                     GAALYYRYRECHEALRPGLIWDICLWISAIALLVAGLFGAGMGLHRLLAH"
     gene            complement(1327..2547)
                     /locus_tag="PKI05_04565"
     CDS             complement(1327..2547)
                     /locus_tag="PKI05_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOA51504.1"
                     /translation="MSEARLAQFSEIAALCRDDDPDFAVHVVEQLINWASSLGASDLH
                     LNPMADCLEVRIRLDGVLHRAGSLPNFAQRKIVTRIKVLADLLTYRTDIPQEGRIRLP
                     GVGPEIRVSTFPSLYGERIVTRFFPEETRFKVLNDLGFPEGIVQILRRALTETSGAIF
                     ITGPAGCGKTTTMYACLRELTEETIPRNLMTVEDPIEVAIPGVIQSQVDPRVGLDLAA
                     ALRYLMRQDPEVVAVGEIRDRTTAEVAVEASLTGHLILSTYHAGGAAEAIGRLLDMGI
                     EPYLIRSGVLVVVSQRLLRQVCECAEWTGDSGQMLGLPVDQVRVAKGCEECRHTGYVG
                     RFPIAEVLVLQDEAIGRAVLDRSDVITLERVAMERGMVTLWENAITAVKEGRTTPLEV
                     RRVLGWGDYWKLTA"
     gene            2789..3847
                     /locus_tag="PKI05_04570"
     CDS             2789..3847
                     /locus_tag="PKI05_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51505.1"
                     /translation="MSESEEPRPEDLQQRCEDEIDRNATDAENVMESAADRNSEGSGT
                     EILKNELVPMDHDEEKRSAFLTGEEILAPEAVEKVEVAAAESSDAPENDSGLELPEDE
                     LLDLPRSTDARVISCTDTDVSSAESADQTGELAQQEALEVETSAVSEPAVTEMTAEEQ
                     PAAEGPSDQEGTGATEVVTTEPEAESTILMVRVPGDERWFPLSEVIVQTTGEPFGESA
                     ARWISRLYRQSLESTPEGPEMSSGPSTAVGEVVFPSITTAPEPTDYRERLKKAAPKKS
                     IVKELLGIVLGGIGGLLIAYYALNWFGGPRYDFAKIPLPGLHHTYKHAPPWLKDLLEG
                     KYWTTSGEDSSADEQVEP"
     gene            complement(3870..4325)
                     /gene="rpiB"
                     /locus_tag="PKI05_04575"
     CDS             complement(3870..4325)
                     /gene="rpiB"
                     /locus_tag="PKI05_04575"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOA51506.1"
                     /translation="MRIVVGSDHRGSTLRHKVIAYLSRLGQEVIDVGVDSQNGVDYPD
                     VAAEVARRISGSEGERGLLICGTGLGMAIVANKFPGVRAAPCHDELMVEIARRHNDLN
                     VLCLAADLVGELLAIRLIDVWLQTEFEGGRHARRLQKIAQIESNGHYRK"
BASE COUNT         1011 a         1246 c         1268 g         1023 t
ORIGIN      
        1 agcgatcaac cgtctgcctg ggccacgctg gcgggtccat tggatgcttt ggtattgggt
       61 ggttatgttt gtggtgagcc ttgctcagct tggtgggatc gcccatggag ttggccaggc
      121 cttggctata gcacttccca ttgatggaag ctttcgacag cttctcaaag agcaggagga
      181 atgggaccga gaggctgcac aaattcgacg ggagttagaa gcatcgtttg aacaaccgaa
      241 ggggtctgga tcggcaggca cggcgagcgt tgacaaaaaa accgttgaaa cagaggtggc
      301 gcagcgcatg caacggcccc gtccgggtta cgaaaaccga gagggcttac tgacggatga
      361 cctaatctgg gccatcatca tctgcttggt gacgattcca attctcgtct atggtcgcta
      421 tgggatgatc caatgggtgt gcacaatcct cgtggtcggt ttcacgctgg tcacgatatt
      481 ctgtgtgatc ggtatccagt tcaagcctga gtgggccatc tcttgggatg atttgcggga
      541 ggggctttca tttcggcttc ccggaactgc caatcgttgg ctagcacttt ctacagcctt
      601 ggccacattc ggaattatcg gcatggggac caacgaattg attacctacc cctattggtg
      661 cctagaaaaa ggctacgccc gcttcacggg accgcgcgat aactcaccgg gttgggcaaa
      721 acgagcgcgg ggatggatgc gggtgatgcg ggccgatgcg tttctgtcga tggtggttta
      781 cacgttcgtg acggcagcct tctatcttct cggggccgct gtcctatacc gttcggagct
      841 gattccagcg ggcagtcaga tgatctctta cctcagcgaa atgtacgtcc cggtgctcgg
      901 tgagtggaca aaggccttct ttttgtttgg cgcgttcgcc gtgttgtatt ccacgttttt
      961 ctcggcaacg gctggccaca cccgtgtggc ggcggacgct gtcgccattg tgcgacgtgg
     1021 aaggtctcag ccggatcgac cgcctcaaag ctgggtgacc gcctttagca ttggctttcc
     1081 gttattcagt ttggtgacgt gccttttcta tcgggaaccc gtgacactca tcgttgccag
     1141 cggcttcatg caagcgatca tgttgcccat gctggccggg gcggccttgt attaccgcta
     1201 tcgagaatgc cacgaagcat tgcgcccagg acttatctgg gatatttgcc tttggatttc
     1261 ggcgatcgcc ctcctggttg cgggactttt cggggccgga atgggcttac accgcctgtt
     1321 ggcacattag gccgtcaact tccagtagtc tccccacccc agaacgcgcc gtacctcgag
     1381 cggggtcgtg cgcccttctt tcaccgcagt tatggcattc tcccaaagag tcaccatccc
     1441 ccgttccatc gctacccgct ccaaggtgat gacatctgaa cgatctaaca cggctcggcc
     1501 aattgcctca tcttggagga caaggacctc agcgatggga aaacgtccaa cgtatcccgt
     1561 gtgacgacac tcctcgcaac cttttgctac tcgcacctgg tccacaggta accccagcat
     1621 ctgacctgaa tcacctgtcc attccgcgca ctcgcatact tgtcgcaaga gtcgttgcga
     1681 aaccacgact agcacaccac tgcggatcag gtatggctca atgcccatat cgagaagccg
     1741 tccaattgcc tcggcagctc cgcccgcgtg ataggtgctc aggatcaagt gaccggtcaa
     1801 agaggcttcc acggccactt ccgccgttgt tcgatctcga atttccccaa ccgccacgac
     1861 ctcggggtcc tgcctcatca agtaccgcaa agcggcagcc aaatccaagc ctacccgtgg
     1921 gtcaacctgg ctttggatga caccgggaat tgccacctca ataggatctt caaccgtcat
     1981 taggttccgc gggatcgtct cttcggtgag ttcccgcaaa caggcataca tggtggttgt
     2041 cttgccacac ccagcgggtc cggtaataaa gatagcaccg gatgtttccg tcaaagccct
     2101 gcgaagaatc tgaacgatcc cctccggaaa accaaggtca ttaaggactt taaaacgggt
     2161 ctcttcggga aagaaacgcg tcacaatacg ttccccgtaa agggaaggaa acgtgctcac
     2221 gcgaatctca ggtcctacgc ctggtagcct gatccggcct tcctggggaa tgtcggttct
     2281 gtacgtcaac aggtctgcca aaaccttgat ccgggtgaca atttttctct gagcgaagtt
     2341 gggcaggctg cccgcacgat ggaggacgcc atccaagcgg atccggactt cgagacagtc
     2401 agccatggga ttgaggtgca ggtcgctggc cccaagactg ctggcccagt tgatcagttg
     2461 ttccacaacg tgaacggcaa aatccggatc atcgtctcga caaagtgctg caatttcgga
     2521 aaactgtgcc agtctggctt cgctcatggc gttgcgtccc ctggtttagg ccggtgtgtt
     2581 cacagagttt ccacgccctg gcggacagat taaaatcaag ttaagtccta tttgatcata
     2641 accgacatga aggttgaaac cgagagggtg gctagggcgg caaggaaagc cggggtagca
     2701 tcttcctaac ttcgtaggcg aggaacgaag cgctcttgga tttccagggg attgttatca
     2761 cagcacgctg ggtctagggg aacagactat gtcggaatct gaagagccgc ggccagagga
     2821 tctgcagcag cgatgtgagg atgaaattga tcgaaacgcc accgacgccg agaatgtcat
     2881 ggaatcggct gcggacagaa attccgaggg ttccgggacc gagatcctca aaaatgagct
     2941 ggttccaatg gatcacgatg aggagaagag atctgctttt ttgacaggtg aggaaattct
     3001 agctccggag gctgttgaga aggttgaggt cgcggcggca gagtcatcag atgccccaga
     3061 aaatgacagc ggcctagagt tgcccgaaga tgagctcctc gatctgccgc gttcgacgga
     3121 tgcccgtgtt atctcttgta cagacacaga cgtgtcttcg gcagagagcg cggaccaaac
     3181 aggggaatta gcacagcaag aagcgttgga ggtcgagaca tcagctgtct ccgagccggc
     3241 tgtcacagaa atgactgccg aggaacagcc agccgcggaa ggcccttctg atcaggaggg
     3301 caccggcgcc accgaggtgg taacgacaga gcccgaagcg gaatcaacga ttttgatggt
     3361 gagagtacca ggagacgagc ggtggtttcc cttaagcgaa gtcattgttc aaactacggg
     3421 tgaacccttt ggtgaatccg cggctcgctg gatcagtcgg ctctatcggc aatcattgga
     3481 atcgacacct gagggaccgg agatgtcatc ggggccgtca accgctgtgg gtgaggtggt
     3541 ctttcccagt atcaccacgg cccccgaacc aacggattac cgggaacgac tgaagaaggc
     3601 tgcgccgaaa aaatcgatcg ttaaagagtt gctagggatc gtgctggggg ggatcggagg
     3661 gctccttatt gcgtattacg cgcttaactg gttcggtgga ccgcgttacg attttgcgaa
     3721 gataccccta ccgggacttc accacacgta caaacatgca ccgccgtggc ttaaagattt
     3781 gctcgaaggc aagtattgga cgacgagcgg tgaggatagt agtgcggatg aacaagtgga
     3841 accgtgacgg acgtttcaac gaagacggtc tatttgcggt aatggccgtt tgactcaatt
     3901 tgggcgattt tttgcaaacg tcgagcgtgg cggccgccct caaattctgt ttgcagccat
     3961 acgtcaatca agcgaatggc caggagttcg ccaaccaggt ccgctgcaag acataatacg
     4021 ttgagatcat tgtgccggcg agcgatctca accatcaact cgtcatgaca cggtgccgcc
     4081 cgtaccccag ggaacttatt ggccacgatc gccattccaa gaccagttcc gcagatgaga
     4141 aggccccttt ctccttcgct gccactaatt cgccgagcaa cttcggcggc cacgtcgggg
     4201 taatccaccc cattctggct atccacaccg acgtcgataa cctcctgccc gaggcgactg
     4261 agataggcaa tcaccttgtg tcgcaaggtg gaacctcgat ggtcgctccc gacgacgatt
     4321 cgcatggtct gcctatctcc cgacgcttat gttacacttg tgtcctcgcc ccattctgcc
     4381 gctcttggcg agcaaaactc caagagacgg ataaccgcag ccatttatcc tgttttttcc
     4441 acgatcaatt ctagacccca gcttaacctt ccactatatt ctcaattccg tcatcttcca
     4501 agaatggtca acacttctgc gaatcacgta aacggtacag ccttccca
//
LOCUS       DAOPTR010000170         4547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_15404_length_4547_cov_3.178094, whole genome shotgun sequence.
ACCESSION   DAOPTR010000170 DAOPTR010000000
VERSION     DAOPTR010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15404_length_4547_cov_3.178094"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..253)
                     /locus_tag="PKI05_04580"
     CDS             complement(20..253)
                     /locus_tag="PKI05_04580"
                     /inference="COORDINATES: protein motif:HMM:NF016223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOA51507.1"
                     /translation="MQVHGPSHIHGAQPLNGPHLSRLGQTSSPSQGAPIRDELHLSPV
                     GQLLDKVHDLPDIRADRVARIRAEIAAGTYERE"
     gene            1314..1532
                     /locus_tag="PKI05_04585"
     CDS             1314..1532
                     /locus_tag="PKI05_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51508.1"
                     /translation="MNGAKFDCSRKSRESHENNVVPIRVSAEAFRKFQESMKEYWEQV
                     IAQHGSPRRLRIGQRIRRRSRKAPKAKR"
     gene            complement(1596..3770)
                     /locus_tag="PKI05_04590"
     CDS             complement(1596..3770)
                     /locus_tag="PKI05_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOA51509.1"
                     /translation="MRSIQRFLLVMVVGIPCVAMALDWRVLPPGEVPADARQEKLKTL
                     DDYFPFTPPSDLKSWEIRRERVQRQILVAAGLWPMPERPPIQAVIHGAVDRGDYTVEK
                     VYFESYPGFYVTGNLYRPKKGQGPFPGVLCPHGHWKNGRFYDAGLEQVRWDIVNGAER
                     FEVGGRSPLQARCVQLARMGCVVFHYDMVGYADSRQLPHRPGVRQAMNTTERWGYFSP
                     AAELHLQNILGLQTFNSIRALDFLLSLPDVDAKRIGVTGASGGGTQTFLLCAVDPRPT
                     VSFPAVMVSTAMQGGCTCENASYLRIDTGNIEFAALFAPKPLGMTAADDWTREIATKG
                     LPELKQLYTLYGVPNNVMAKPLLHFPHNYNYVSREVMYHWMNKHLSLGLKEPIIEEDY
                     HPLSQEEMTVWDAAHPAPPGGEEFEREFLARLTEATRRQMESLIPDSPEAWQKYANVI
                     GGAYDVMIGRGLPENEDLSVDVRRETQEDSVTARYLLIHQPSRKETVPVLVLVPASPR
                     PTVVIWVSPNGKQGILAEDGKPIPVIRKMLEQKLVVVTADLFGQGETTPDGKNVERQR
                     LVRDDYAGYTFGYNPPLYAQRVHDLLKVIRFARSQLVPEGKLIMIGPQGAAHWVAGAL
                     AQAKGAVDLTVVDTGGFRFQSINAFDHPDFFPGAAKYLDLPGLLSIAAPAKLWLAGEN
                     SEGLSLIRRVYHAAPAEGQLRAWDSLPMDENSEWLKSLIESL"
     gene            complement(4102..>4547)
                     /locus_tag="PKI05_04595"
     CDS             complement(4102..>4547)
                     /locus_tag="PKI05_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOA51510.1"
                     /translation="EQGASFTTTQNRLAALLAVQNTYLSTFQTLGGFGVLLGTVGLGA
                     VVFRGLWERRREIGLLRAVGFRSRQIVYLVMSENLGLLITGLVIGAGAALVAISPQLA
                     TGRATIPYKSLLQMLMAMMAIGTLTAWIATHWLLRQNLIASLRRE"
BASE COUNT         1010 a         1277 c         1248 g         1012 t
ORIGIN      
        1 caagggccac ttgcagtttt tattctcttt cgtacgtacc ggcggcaatc tcggcgcgga
       61 tccgggcgac tcggtcggcg cggatatcag ggaggtcgtg caccttgtcc agaagctgcc
      121 ccaccgggga taaatgcaac tcatcccgaa tgggagcacc ctggctcggc gaagaggtct
      181 gtcccaatcg agacagatgc ggcccattca ggggttgagc accatgaata tggctgggtc
      241 catggacttg cattcccggt ctcctctctc gtcgcgtaac ctcagttgtt tcgcaacacc
      301 gattgccgat gacctcacgg cgccgcccga gaacatatcc ggcggacgtc ttgctccata
      361 ctttctctac aaaactatac tccagccttg ggatttatgc cagagcgaat accgagactt
      421 gggcttgatt atctttgcca tttgcgccag ttgcccattt tatggcgcaa tatcagagca
      481 ttttggctag atcacaatcc gttgagatga gcctgtccct ggccgccaca cttcgccctc
      541 cacataaaca tctttcggct gagtacgcac actatgctcg caaaactctc caagagacct
      601 gtcgtaggaa agataccaaa gggacacaaa cgacgaaggc aacgaaatgc acgccctgtt
      661 ccattttcaa aggttgcgcc actgagcctg ggatttcgtc ttcgcaacaa tttcttaacg
      721 tgcccctcgg agcacggtaa gggaaagggc ttggccgcga attggtagta ctagcgcttg
      781 gactctcacg gccgctccga aacctgccta aaccactcgg gtttatttct tagataccta
      841 atggctgcgg gccccagcca gcttttttgc acatgaccct aacaccacca gcccgtggtg
      901 tggcgcccgg cgggattgtt cccactgggc gtttatttta ttgaaatgct tatctatcaa
      961 gtagtatctt gctgttttgt gagattcttg gtcgcttgga gatcttcttg gcggcgaggt
     1021 cagttcttgg ggaaacttgc ggcttgttgg cttctccggg tttgcagcga accagaatct
     1081 cgcccggcac gtccaagagt tagaaggcca gaaaccaggt tatctgggac tggcgaacca
     1141 cagggaccgg ctggggaata ggtttttcct tcgagtgtca acagtttgtg gggcgatcca
     1201 caaatcagag ggttctagaa aatcgttttc acattccttt cctttggcgt ccctcgtgga
     1261 taggatggaa acaagtgaga cgagaatcgg ggccttaatt ttggggtgaa ctaatgaacg
     1321 gtgcgaaatt cgattgcagc agaaaaagtc gggaatcgca cgaaaacaac gtcgtgccga
     1381 tccgcgtgtc ggccgaggct tttcggaagt ttcaagagtc aatgaaggaa tactgggaac
     1441 aggttatcgc ccaacatggg tcaccgcgtc gcttgcggat tgggcagcga attcgccgcc
     1501 gttcgcggaa ggccccaaaa gctaagcgat aagcgtttat ccggctgtat gcctttttgt
     1561 gcacttatcc tattttctct aaattgccca aagccttaga gggattctat caagctcttg
     1621 agccattcgg agttttcatc cattggaagt gaatcccaag cccgcaactg tccttcggcc
     1681 ggtgcagcat gatagaccct tcgaataagg gagagccctt cgctattctc cccagctagc
     1741 cacaacttcg ccggtgccgc aattgacagc aatccgggga gatcgaggta tttggccgcc
     1801 ccggggaaaa agtcgggatg gtcgaaggcg ttgatcgatt gaaaccggaa cccaccggta
     1861 tctaccaccg ttagatcaac ggcacccttg gcttgggcta gggcacccgc cacccaatga
     1921 gcggcaccct gggggccaat cataatgagt tttccctcgg gaaccaattg cgagcgggca
     1981 aaccggatga ctttgagcaa gtcgtgaacc cgctgggcat acaggggcgg attgtaccca
     2041 aacgtgtagc ccgcgtagtc atcccgcacg agacgttgcc gctcgacatt cttgccgtcc
     2101 ggggtagttt cgccttggcc aaaaaggtcc gctgtgacca caacaagttt ttgctctaac
     2161 atctttcgga tgacgggaat aggcttgcca tcctcggcaa ggatgccttg cttgccgttg
     2221 ggactgaccc agatcacgac cgtcggtcgg ggcgatgcag gaactaggac caagactggc
     2281 acagtttcct ttcttgacgg ttgatggatc aaaaggtacc gggcggtaac actatcctcc
     2341 tgcgtttccc gccggacgtc cacgctcagg tcttcgttct ctggcaaccc gcggccgatc
     2401 attacgtcgt aagcccctcc tatcacgttg gcatactttt gccaggcctc gggagaatca
     2461 ggaatgagcg attccatctg tcgccgagtt gcctcggtga gacgtgccag aaactctcgc
     2521 tcaaactcct ctccgccagg tggcgctggg tgcgctgcgt cccagactgt catctcttcc
     2581 tggctaaggg gatggtagtc ttcctcgatg ataggctcct taaggcccag cgacaggtgc
     2641 ttattcatcc agtggtacat gacttcgcgc gaaacatagt tgtagttgtg cggaaagtgg
     2701 agaaggggct tggccatcac attgttggga acgccgtaca acgtgtaaag ctgcttaagt
     2761 tcgggtaacc ctttggttgc gatttcccta gtccagtcgt ccgcggccgt catcccgagg
     2821 ggttttgggg caaagagggc tgcaaactcg atattgccgg tatcaatccg cagataggaa
     2881 gcgttttcgc acgtgcagcc gccttgcatg gccgtagaga ccatcaccgc ggggaacgag
     2941 acggtgggac gggggtcaac agcacacagc aagaaggttt gcgtgcctcc gccactggcc
     3001 cccgtcactc cgatccgttt tgcgtccacg tctggcaagc tgaggagaaa atccaaagcc
     3061 cgaatggagt tgaacgtttg cagtcccaat atgttttgca ggtgcaactc cgctgccggg
     3121 ctaaaatagc cccagcgctc cgtcgtgttc atggcttgcc ggacgccggg gcgatggggc
     3181 agttgtcggc tgtcggcgta gccaaccatg tcgtaatgga atacgacgca ccccatccgt
     3241 gctaactgaa cgcacctcgc ttgaagtgga gatcgaccac cgacttcaaa gcgttccgcc
     3301 ccgttcacga tatcccagcg gacttgttcc aatcccgcgt cataaaagcg accgtttttc
     3361 cagtgaccgt gcgggcaaag aacacccggg aaaggtccct ggcccttctt tgggcggtaa
     3421 agattcccgg tgacgtaaaa ccctggatag ctctcgaaat aaaccttctc cacggtgtaa
     3481 tcgccgcgat caacagcacc atggatgacc gcctggatgg gaggtcgttc cggcatcggc
     3541 caaagccctg cagccaccag gatttgccgc tgaacacgtt cgcgccgaat ctcccaggat
     3601 ttgagatcgg aaggtggggt aaacgggaaa tagtcgtcga gcgtcttcag tttttcctgg
     3661 cgggcatcgg cggggacctc tccgggcggc aacacacgcc aatcgagggc catcgccacg
     3721 cagggaatcc cgaccaccat gaccaaaaga aatcgctgaa tactccgcat gaccatgacc
     3781 tccgcaggca gaagacaacc cgatcgttac cgagcttttc ctaagatcac cgtgctggcg
     3841 gattttataa gccactttcg cccggttttc aatcccgcgt ggttctgagc gatccaggat
     3901 tcccctgggt atgaggcacg aggacaacat tcgacgtgcc ctcgagcgga tccagtgagg
     3961 gtttctcctt ttggttgagg cgcacttcta gggcgcggat agattcgttg cccgtcgcgg
     4021 ctggtcgaaa caaaggcctt gcctgaggag aatcgtctca gacgagggca gagaagatcc
     4081 cggaattgtg tcaccaaagg ttcactcgcg tcgcagactg gcgatcaggt tctggcggag
     4141 caaccaatgg gtagctatcc acgccgtcag tgtgccaatc gccatcatcg ccattaacat
     4201 ttgaaggagt gatttgtaag ggatggttgc acgtcccgtg gccaactgcg gcgagatcgc
     4261 cacgagcgcc gcaccggcac caataacgag ccccgtgatc aacaatccaa ggttttctga
     4321 catcacaaga tatacgattt gccgcgaccg gaacccgact gcccgaagga gaccgatttc
     4381 gcggcgtcgt tcccacaatc cccgaaagac gaccgcaccc aagccgaccg tcccaaggag
     4441 cacaccaaat ccaccaagcg tttggaacgt ggagagatag gtattctgga cggctaacaa
     4501 ggcggccaaa cgattttgag tcgtcgtaaa gctggccccc tgctcag
//
LOCUS       DAOPTR010000171         4546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_15409_length_4546_cov_3.318192, whole genome shotgun sequence.
ACCESSION   DAOPTR010000171 DAOPTR010000000
VERSION     DAOPTR010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15409_length_4546_cov_3.318192"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1449)
                     /locus_tag="PKI05_04600"
     CDS             complement(<1..1449)
                     /locus_tag="PKI05_04600"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOA51511.1"
                     /translation="MMKSNIVCFLPGAMLAISLAASTVAAETTIYLSPQGKDTNRGTA
                     DQPVATLARAMELVKAVRQRDGAAADVRVLLAEGIYRIAEPVTISPEHSPGPGKLVIC
                     GEAGKKVVISGGRVISGWQKVGNRFEVVIPEVAQGKWIFRELFVNGRRATRARSPNEG
                     FVRIDKAGPDRRTSLFIYPQDAWLFKGKISGAEFVYLHDWSTSRVKIAGFHPDTLEVT
                     FSQRVGCSAPHYAIGNWPHARYFVENSEDFLDAPGEWFLDDGTGKLIYLPREGESLET
                     LEAIAPVAPALFSIVGDLDHGKPVTNLTIEGITFEHCRFDLPAAGYAAGQATVYEPRV
                     NGGAGREIMPAAVTFDFAKNCQLTRCHFQHLGSSGVWFRRQCRQCTVERCVFDDISGN
                     GINIGETMTRPKSEAPVDWESPYDNSCTWGIMVRDNRITHCGRQFFEAVGVWVGIAAG
                     NVIEHNEIGYLPYTGVSVGWSWNDSPTGCRVNT"
     gene            complement(1565..2524)
                     /locus_tag="PKI05_04605"
     CDS             complement(1565..2524)
                     /locus_tag="PKI05_04605"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOA51512.1"
                     /translation="MTHRALITGITGFVGRHLYAHLVECGDQVLGLAPSPVPKVPSWG
                     RLPDDGEILIWDFAESKECPDDIKARIRQFDPTVVYHLAAISVPEDCGHTAPNERAWA
                     INVEGTRRIIELLNETSSGPRFIFVSTSHLYRVPQDKVTFVDEEYPANPRNAYAQTKW
                     AAEQIVQAAVKEDRLNAIIVRAFPHSGPGQSERMMLPSWAAQFARGMQTIQVHTLQAT
                     IDLCDVRDVVRAYRLLADSAVPGEVYNIGSGVPRTSAQVFELLRLLANPHCEVVETHP
                     GIKYDPIADLRKVQSHTHWKPEIPPEQTVADTYRWWLATVGRK"
     gene            complement(2617..>4546)
                     /locus_tag="PKI05_04610"
     CDS             complement(2617..>4546)
                     /locus_tag="PKI05_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOA51513.1"
                     /translation="IREIPFQQQRDKIEEKIAELVNEGRISGIATIRNESDLKEPVRI
                     VLELKRDADPEIVLNQLYEYSPLQQTYSINLLALVDGKPRLLNFKELLQEFLRHRVTV
                     IRRRTQYLLGKARQRKHTVEGLLLALANIDEVIQVIRTSETQAEAKERLMKIACPAAM
                     LARALGEAGFEQFRSERGVSEEYFLTAVQADAILRMTLGQLVNLEQEKLAEEHRRLLA
                     DIAEYNRILSDERNILAIIREEQLAIKQKYGDARRTEIIDQEVGSFDMEELIPDEMMV
                     VSISSAGYIKRTPVSAYRAQRRGGKGLKGARADDEDPIEHVFLGSTHDYLLFFTNLGR
                     VHWRKVYELPELPRDAKGRAIVNLLNLQDNERVASCRPVRDFSLPNHYLLFVTMKGLV
                     KKTELSAYGRPVRGGIIAIKLREDDELVGVVITKPGDEIVLSTANGMAIRFRESDVRP
                     MGRAATGVKGITLDPNDRVVGVVVAEPDACLLTVCANGYGKRTPFGPNSEDEGEELHE
                     DLDEVTEGTDELAAKSEELSEEDETDDDRDEPGSQYCYRTQRRGGKGIRDIKTGGRNG
                     PVVGIVAVHDDDEVLMMTAKGKIQRIRANEIRKTGRNAKGVRLMTLDEGDQIVAVKRI
                     PAEPTNGSDAEGNTPTTS"
BASE COUNT          984 a         1302 c         1188 g         1072 t
ORIGIN      
        1 agtgttaacc cgacacccgg tgggcgagtc attccaactc catcccacgg atacgcccgt
       61 gtagggtagg tacccaattt cattgtgctc gatgacgttg ccagcagcga tcccgaccca
      121 cacgccgaca gcttcaaaaa actggcggcc gcaatgggtg atccgattat cccggaccat
      181 gattccccat gtgcaactgt tatcgtaagg tgattcccaa tccaccggag cctcagactt
      241 gggacgtgtc atcgtctcgc cgatattaat tccattcccc gaaatatcgt cgaaaacgca
      301 cctctcgacg gtacactgac ggcattgccg gcgaaaccat acgcctgatg agcccaggtg
      361 ttggaagtga cagcgagtca gttggcagtt cttggcaaaa tcaaatgtca cggccgcggg
      421 catgatctct cggccagctc ctccattcac tcgcggttcg taaacggtcg cctgaccggc
      481 ggcataaccg gcagccggta gatcgaaccg gcaatgctca aaggtgattc cttcaatggt
      541 gaggttggtg acaggttttc cgtggtctaa atccccgacg atgctgaaaa gggcaggagc
      601 aaccggcgca atggcttcca gcgtttccaa ggattctcct tcccggggca aatagatcag
      661 tttgcctgtg ccatcgtcca ggaaccattc acccggggcg tccaggaaat cctcactgtt
      721 ctcgacaaaa taacgggcat ggggccagtt tccgatggcg taatgaggag cggagcaccc
      781 caccctttgc gaaaaagtaa cctcaagggt atcggggtga aaacccgcaa tcttcacccg
      841 cgatgtggac caatcgtgta aataaacaaa ttcggccccg gaaattttgc ctttgaacaa
      901 ccatgcatct tgcggataaa taaacaggct ggtgcggcgg tctgggcccg ctttgtcgat
      961 acggacaaat ccctcattcg gcgatcgagc tcgcgtcgcc cggcgaccat tcacgaaaag
     1021 ctcgcgaaaa atccatttcc cctgagcaac ctcaggaatg acaacctcaa aacgattccc
     1081 gaccttttgc caacccgaaa taacgcggcc gccactaata acaacctttt tgccggcttc
     1141 accacaaatt accagtttgc cggggccagg tgagtgttcc ggcgagatcg tgacaggttc
     1201 ggcaattcga taaatccctt ccgctagaag gacacggacg tcggctgcag ccccgtcacg
     1261 ctgtcggacg gccttgacga gctccatggc gcgggcgagc gtggcaacag gctgatctgc
     1321 cgttccccgg ttagtatctt ttccttgcgg cgagaggtaa atcgtggttt ccgcggctac
     1381 ggttgatgcc gccagcgaaa tggctagcat cgccccaggt aggaaacaaa cgatgttcga
     1441 tttcatcatg gcgtctctcc cttcacgctg tctttccgaa ctggacgcta caccatcccc
     1501 gcggagaaca aaaacgttta cgtttccgac agccgagtcc taaagcaaga gggcaacggt
     1561 accgctactt gcgccctact gttgctaacc accagcggta ggtatcggcc acggtctgct
     1621 cggggggaat ttctggcttc caatgggtgt gactttgaac cttccgcaaa tcggcgattg
     1681 gatcatactt gattccagga tgagtttcaa caacctcaca atggggattg gcgaggagtc
     1741 gcaatagctc aaaaacctgg gcacttgtgc gcggcacacc agaaccgatg ttatagacct
     1801 caccaggcac cgcagagtct gccagaaggc ggtatgcccg cactacgtcc cgaacgtcgc
     1861 acagatcaat cgtcgcctgc aacgtgtgaa cctggattgt ctgcatgccc cgcgcaaatt
     1921 gggcagccca tgagggaagc atcattcgct cggattgtcc tggacctgaa tggggaaatg
     1981 cgcgaacgat gatcgcatta agcctatcct ccttgacggc agcttgtaca atttgttccg
     2041 ctgcccattt ggtctgggcg taggcattgc ggggattagc ggggtactct tcatcaacaa
     2101 atgtcacctt gtcctgcggg acgcgataaa gatggcttgt gctcacaaag atgaatcgcg
     2161 ggccactact ggtctcattc aacagttcga tgatgcggcg tgttccttcc acgttgatgg
     2221 cccacgccct ttcattcgga gccgtgtgac cgcagtcctc aggcacagat atagctgcca
     2281 agtgatagac aaccgtgggg tcaaactggc gaatgcgggc cttgatgtcg tccgggcatt
     2341 cttttgattc cgcgaagtcc cagataagaa tctcgccatc gtcaggaagg cgaccccaag
     2401 aaggcacttt gggaaccggg gatggtgcaa gtcccagaac ctgatcacca cactctacaa
     2461 gatgcgcata caggtgacgg cctacaaaac cggtaatacc tgtaatcaga gcacggtgag
     2521 tcatgcgcat ttaactggat taccggtcca caatgtcgcg gctgacgtca caatgggtcc
     2581 tacgggctgt ttttcttcgt gtcaaatctg gctcaatcag gaggtggttg gcgtgttgcc
     2641 ttcggcatct gaaccgttgg tcggctcggc tggaatcctc ttgacggcca cgatttggtc
     2701 tccctcatct agtgtcatca gtcggacacc cttagcattg cggccggttt tccgaatctc
     2761 attggcccga atcctctgaa tcttcccttt tgctgtcatc atcaagactt cgtcgtcgtc
     2821 gtggactgcc acgataccga ctacggggcc attcctaccg cctgtcttga tgtcgcggat
     2881 gcccttgcct ccacgtcgtt gcgtccgata gcagtattgg gaacctggtt cgtcgcggtc
     2941 atcatcggtt tcgtcttcct cagaaagttc ctcgcttttt gccgccagtt cgtccgtgcc
     3001 ttctgtcact tcgtccaaat cttcatgaag ttcctcacct tcgtcctctg agttaggccc
     3061 aaatggcgtt cgcttcccat agccatttgc acaaacggtt agcaagcatg cgtctggttc
     3121 ggccacgacc actcccacga cccggtcgtt cggatccagc gtgatacctt tgacgccggt
     3181 tgcagccctt cccattgggc gaacatcgga ttcgcgaaac cggatcgcca ttccgttggc
     3241 cgtggatagc acgatctcat cccccggttt ggtgatgaca acgccaacca gttcatcgtc
     3301 ttcgcggagt ttaatggcga tgataccgcc gcggacggga cggccatagg cagacagttc
     3361 cgtctttttg accagccctt tcatcgtcac aaacagaagg taatgattgg gcagcgaaaa
     3421 atctcggacc ggacggcagc ttgcgacccg ttcgttgtcc tgcaaattga gcaaattgac
     3481 aatcgcccgc cctttggcat cccgaggcaa ctctggcaat tcatacactt ttcgccaatg
     3541 cacgcgaccg agattggtga agaacaaaag ataatcgtgc gtgctgccca gaaacacatg
     3601 ttcgatggga tcttcatcgt cggcgcgggc acctttgagt cccttgccgc ctcgcctttg
     3661 cgcccgatat gccgagacgg gcgtcctttt gatgtagcca gccgaactga tcgagacgac
     3721 catcatttcg tcggggatca gttcctccat gtcgaaggac cccacctcct ggtcgatgat
     3781 ctctgtgcgg cgggcgtcac catatttttg cttgatggcg agttgctcct ctcgaatgat
     3841 cgccaaaatg ttgcgttcat cggacagaat gcggttatac tcggcaatgt ccgccagcag
     3901 acggcgatgc tcctcagcca gcttttcttg ttctaggttg actaactgcc ctagtgtcat
     3961 gcgtaagatg gcgtcggcct gtaccgcggt taggaagtac tcctcggaca ccccgcgttc
     4021 ggaccggaat tgttcaaatc ccgcctctcc caaggcccga gctaacatgg ccgccggaca
     4081 agcgattttc atgagccgtt ctttggcttc cgcctgtgtt tctgatgtcc gaatgacttg
     4141 aatgacttcg tcgatatttg ccaaggcaag cagaagcccc tccacggtgt gtttgcgctg
     4201 ccgagctttg cccaacagat actgtgtccg tcgtcgaatg acggttacac gatggcgcag
     4261 aaattcctga aggagttcct tgaaattgag caaccgtggt ttcccatcta cgagcgctag
     4321 gagattgatg gagtaggtct gctggagggg ggaatactcg tacagttgat taaggacgat
     4381 ctccggatca gcgtcacgct tcagctcaag gacaatccgg acgggctcct taagatcgct
     4441 ttcgttacgg atggtcgcaa tgcccgagat gcgcccctcg ttgaccagtt cggcaatttt
     4501 ttcttcaatc ttgtctcgct gttgttggaa tgggatttcc cgaatg
//
LOCUS       DAOPTR010000172         4545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_15413_length_4545_cov_3.885301, whole genome shotgun sequence.
ACCESSION   DAOPTR010000172 DAOPTR010000000
VERSION     DAOPTR010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15413_length_4545_cov_3.885301"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1755
                     /locus_tag="PKI05_04615"
     CDS             <1..1755
                     /locus_tag="PKI05_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51514.1"
                     /translation="EEPRFAFVEELSRPRRAVPPQPSTSTQEAPRYLYSYFGPYGDPL
                     SDPRIDPYPDGLLERLAELGVNGVWLHVVLRNLAPGEPEFPEFGAGCEVRLENLRKLV
                     ERAKRFGIGIYLYINEPRAMPREFFRNYPEIAGVAEGEYVTMCTSHPKVRAWLTRSLQ
                     YVFSQVPNLAGVFTITASENLTNCASHGRHKDCVRCRDRSADEIIAEVNAAIEAGVHA
                     ASPRAHVLVWDWGWHGHGDASSLIPLLPKSVWLMSVSEWGQSFERGGVAGQVGEYSLS
                     VVGPGPRALKHWKVAQENGLKTAAKLQLGATWELSAVPFVPVLDLVAEHCSRLAQVGV
                     DGCMLSWTVGCYPSPNLAVANRLLSDKSATPDAVLNTVAQQRYGPRAVGAARQAWRLF
                     STAFQEYPFHIQVVYRGPQHWGPANLLFAEQTGYRATMVGFPYDDLDGWRGPYPREVF
                     AQQFSRVATIWADGLREFAKVVALADEDKKPTAEADYRVAQAAQIHLASVANQARFIM
                     ARNRYYSARDAADRAQAAATLDEILTAEISLAKQLYSITKCDSRIGFEASNHYYYVPL
                     DLVEKVINCEYLRQQFRQ"
     gene            1824..>4545
                     /locus_tag="PKI05_04620"
     CDS             1824..>4545
                     /locus_tag="PKI05_04620"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOA51515.1"
                     /translation="MDIFRHCCVGVCLAIITAVISVSRGEPADSSSVLPPGVKAVWDV
                     NKAFREVTATRERICINGLWQWQPAERGAETVPQDHWGYFKVPGAWPGITDYMQKDCQ
                     TVHAHPAWGNIRFGSLTAAWYRRTITIPASWANRRIALIAEYVNSLATVFLDGQKVGE
                     IVFPAGELDITSAVRPGETHTVSILVEALPLKGVMLVHRDSNTARQVQGSVNRRGLCG
                     DVWLVAQPQGVRIEAIRVETSIRKNLVRFVANLKNAEANKKYSLRAIVKEGTSVVKEF
                     VSPASLVDTTAESQLVLEASWRPEKLWDIHTPQNQYTVEVALLDERGQVLDAAWPERF
                     GFREFWIDGRDFYLNGTRIFLCAVPLDNAQIGAAWASYQGARESLQRLKSFGINFVYT
                     HNYDCQPGSHLSFSEILRAADDVGMLVALSQPHFAQYDWQAENAEETNGYRQHARFYV
                     QVAGNHPSVVMYSTSHNATGYNEDMNPDLIDGIHDPRDGWSLNNARRALRAEAILRQL
                     DPTRIVYHHSSGNLGSMHTSNFYPNWVPIQEMCDWFEHWATVGVKPFFTCEYGAPFTW
                     DWAMYRGWYKGQREFGSAVVPWDFCLAEWNAQFLGDRAYNISDQERRNLKWEAAQFRS
                     GRLWYRWDYPHQLGSSDFDERYPIFAQYLTDNWRAFRTWGVSAISPWEYHIYWKMRPG
                     LARNQRTELSTDWENLQRPGYSPDYLEVRYERMDIAYEPSDWVPTLAAQAMYRNNAPL
                     LAYIAGGSTAFTAKDHNYLPGEVIEKQLIVINNSREPAEVKCIWQFGTVSDRQATRIK
                     GKGHLPVLIQEEIRPASVLLQGGDTLSVETGQQARAMVHLNLPKDLSPGTYVLWAEFQ
                     FDNGDKQKDQFLVNILPLPDDQQLSAAVAQAAGRIGLFDPLGETRKLLDGL"
BASE COUNT          972 a         1184 c         1341 g         1048 t
ORIGIN      
        1 gaagagccgc ggtttgcctt tgtcgaggaa ttaagtcgtc cacgacgggc ggtgccacct
       61 cagccgtcaa catccacgca agaagccccc cgctatcttt actcgtactt tgggccctac
      121 ggtgatccgc tgagcgatcc gaggattgat ccctatcctg atggcctgct ggaacgcttg
      181 gcggaattgg gcgtcaacgg tgtgtggtta cacgtggtct tgcggaacct cgcgccagga
      241 gaaccagaat ttcccgaatt cggggccggg tgtgaagtgc gactggagaa tcttaggaag
      301 ttagtagaac gtgccaagag gtttggaatt gggatctatc tttatatcaa tgagccgcgc
      361 gccatgcccc gagagttctt ccgtaattac cccgagattg ccggtgttgc ggagggtgag
      421 tacgtaacaa tgtgtaccag tcatcccaaa gtccgtgctt ggctgacgcg ctccttacaa
      481 tatgtttttt cgcaggtccc caaccttgca ggggtgttta cgatcacggc ctcggagaat
      541 ctcaccaatt gcgcgtcgca tgggcgtcac aaagattgcg tgcgatgtcg ggatcgttca
      601 gctgatgaga tcatcgccga ggtcaacgcg gccatcgagg ccggtgtgca cgctgcttcg
      661 ccgcgggccc atgtcttggt atgggattgg ggctggcacg gccatgggga cgcctcgagc
      721 ctcattcccc tcttacccaa gtcggtttgg ttaatgtcgg tcagcgaatg gggacagtct
      781 tttgaacggg gtggcgttgc tggtcaggtc ggcgagtatt cgctgtccgt tgtggggccg
      841 gggcctcgcg ccctgaaaca ttggaaggtg gcccaggaaa acggtctgaa aaccgccgcc
      901 aagttgcaat tgggggctac ttgggagttg tccgctgttc ccttcgtgcc ggtgctcgat
      961 ttggtagccg aacattgctc gcggctagcc caggtgggtg tggacggttg catgttgagt
     1021 tggacggtgg gctgctaccc ttcgcctaat ctggcggtgg cgaaccgcct gctctctgac
     1081 aagagtgcaa ccccagatgc cgtcttgaat acagtcgccc aacagcgcta tgggccgcgc
     1141 gcagttgggg cagcccggca ggcgtggcgg ctctttagta cggctttcca ggaatatcca
     1201 tttcacatac aagtggttta ccgcggtccc cagcactggg gcccggccaa cctattgttc
     1261 gcggagcaaa ccggctatcg ggctacgatg gtaggatttc cgtatgacga cctggatggt
     1321 tggcggggac cttatccccg cgaggtgttc gcccagcagt tcagtcgggt tgctaccatc
     1381 tgggctgatg gattgaggga atttgccaag gttgtcgccc tggccgatga ggataagaag
     1441 cctacagccg aagcggatta ccgggtagct caagcggccc aaatccacct ggctagcgtg
     1501 gccaaccagg cgcggtttat catggcccgc aatcggtatt actcggcccg agacgcggca
     1561 gatcgcgcgc aggcagcggc aacactcgac gaaatcctta cggccgaaat ctcgctggcc
     1621 aaacaactgt acagcattac caaatgtgac tcgcggattg gtttcgaggc gtcaaaccat
     1681 tattattacg tcccgttgga cctagtggaa aaggtcatca attgcgaata cttgcggcag
     1741 cagtttaggc agtaatattg atcgcgccgc gggctttcgc tggccgctcc aatgtacgga
     1801 gttcaaattg ggggaggtga gtcatggaca ttttccgtca ttgctgtgtt ggcgtttgct
     1861 tggccattat cacggctgtt ataagtgtga gccgtggaga accagcagat tcttcgtctg
     1921 ttcttcctcc aggagtgaaa gcagtctggg atgtaaacaa ggcctttcgc gaggtcactg
     1981 caacccgcga acggatctgt attaacggcc tgtggcagtg gcagccggct gagcgcggtg
     2041 cggagacggt tcctcaagat cattggggat atttcaaggt ccccggggca tggccgggaa
     2101 tcactgacta catgcagaag gattgccaaa ccgtccatgc tcatcctgcc tggggcaata
     2161 tccgtttcgg cagcttaacg gcagcatggt accggcggac gatcacgatc ccggcttcgt
     2221 gggcaaaccg gagaatcgca cttattgccg aatatgtgaa ttcactggcg acggtctttc
     2281 ttgatgggca aaaagttgga gagatcgttt ttcccgctgg tgagctggat atcacttccg
     2341 ccgttcgccc gggggagacg cacactgtgt caattcttgt ggaagcatta ccgctcaagg
     2401 gagtgatgtt ggttcatcgc gatagcaaca cggcccggca agtccaaggc tcggtcaatc
     2461 gtcgtggcct gtgtggcgat gtgtggctcg tcgcccaacc gcaaggggtt cgaatcgagg
     2521 caatccgcgt tgaaacctct attcgtaaaa acctcgtgag gtttgtcgca aatttgaaga
     2581 acgcagaagc aaacaaaaag tattcactgc gggccattgt caaggagggc acctcggttg
     2641 ttaaggaatt tgtgtccccg gcctcgcttg ttgacaccac agctgaaagc cagctcgtcc
     2701 tggaagcatc gtggcgccca gagaagttgt gggatattca tacgccacaa aatcaataca
     2761 cggtcgaggt tgcgctattg gatgagcgag gccaggttct cgacgccgcg tggccggagc
     2821 gattcggatt tcgcgagttc tggattgatg ggagagactt ttacctcaat ggcacgcgga
     2881 ttttcctctg tgctgtgccg ctcgataacg cccaaatcgg cgcggcctgg gcctcttatc
     2941 aaggggcccg cgaaagtttg cagcggctga aaagcttcgg aatcaatttt gtgtacacac
     3001 acaattatga ttgccagccc ggttcacatc tgagtttttc agagattctt cgggcggctg
     3061 acgatgtggg aatgcttgtg gccttgtctc agccgcactt cgcccaatat gactggcagg
     3121 ccgagaacgc cgaggaaacg aatggttatc gccagcatgc ccggttttat gtacaggtgg
     3181 ccggcaatca tccctcggtc gtgatgtatt cgacgagcca taatgcgact ggttataacg
     3241 aagacatgaa tcctgatctt attgatggca tccatgaccc ccgggatggt tggtcgctga
     3301 acaatgcccg gcgtgcattg cgggcagagg ccattctgcg gcaactcgat cccacacgaa
     3361 ttgtttacca ccactcgtcg ggtaacttgg gaagcatgca taccagcaac ttttacccca
     3421 actgggtacc tattcaagag atgtgtgatt ggttcgagca ctgggccacg gtcggagtca
     3481 agccgttttt tacatgtgaa tatggagcgc ccttcacctg ggattgggcg atgtatcggg
     3541 gttggtacaa gggacagcgg gagtttggca gcgcagttgt gccatgggac ttctgcctgg
     3601 cggagtggaa cgcgcaattc ctcggcgacc gagcttacaa catcagtgac caggagagac
     3661 ggaacctcaa gtgggaagcc gcgcagtttc gctcaggtcg tctttggtat cgctgggact
     3721 atccgcacca gttgggatcc agtgacttcg acgagcgata tcctattttc gcgcagtatt
     3781 tgacggataa ctggcgggca tttcgcacct ggggtgtttc cgccatttct ccatgggagt
     3841 atcatatcta ttggaaaatg cggccggggt tagcgcggaa ccagcggacc gaactttcca
     3901 ccgactggga aaacctgcaa agaccaggtt atagccccga ctaccttgag gtacggtacg
     3961 agcgaatgga catcgcctat gagccatccg attgggttcc caccttggcc gcccaagcca
     4021 tgtaccgcaa caatgctcca ctattggcct acatcgccgg cggtagcact gctttcaccg
     4081 ctaaggatca caattaccta ccgggcgagg tcatcgaaaa acagctcatt gtcataaata
     4141 actcacgcga gccggcagag gtaaaatgca tctggcaatt cggtaccgtg tcagatcgcc
     4201 aagccacgag aatcaaaggc aaggggcatc ttcccgtact catccaagag gagataagac
     4261 cggcatccgt gcttctgcaa ggtggtgata cgctttctgt cgaaacaggc caacaggctc
     4321 gtgcgatggt gcatcttaac ttgccgaaag atctttcgcc tgggacgtat gtgctatggg
     4381 cggagtttca gttcgacaac ggtgacaaac agaaggacca gtttctggtc aacattttgc
     4441 ccttgccgga cgatcagcaa cttagtgcag ccgtggccca agcagcagga cgaatcggtc
     4501 tctttgatcc cctcggcgag acacgcaagc tgttagatgg tttga
//
LOCUS       DAOPTR010000173         4536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_15451_length_4536_cov_3.834635, whole genome shotgun sequence.
ACCESSION   DAOPTR010000173 DAOPTR010000000
VERSION     DAOPTR010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15451_length_4536_cov_3.834635"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1433
                     /locus_tag="PKI05_04625"
     CDS             <1..1433
                     /locus_tag="PKI05_04625"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOA51516.1"
                     /translation="GEWLAEAASKEDLFKPSSGLRWWFAEVDEEKTTIWAQFPGVDPN
                     RETVEINVRQTVFYPRTPGCNYITVRGFIMRHAATPWAPPTAQQIGLIGTHWSKGWII
                     ENNVISHSICCGITLGKYGDEFDNTSANTAEGYVQTINRALANGWHRDNIGHHIVRNN
                     IISHCEQAGIVGSLGAAFCEVTDNVIHDIHMRRLFSGAEMAGIKFHAAIDTLIARNHI
                     FRTCRGLWLDWMAQGTRVHANLFHDNDEDLFVEVNHGPFVVDNNLFLSKLNLRDMSQG
                     GAYLHNLFAGAITVCPEPNRKTPFHPPHKTEVAGLASIQGGDNRFLNNVFIGRGENTP
                     MPEGAPAPRRTWITGFGLSAYTAQAMMNLTEGNIYLDHAPPAQNEANSLEIKEAPFCR
                     LMADEGMVFLTLTTSADWANAGREIVTAARLGQTAVSQLPYENYDGRNLVIDTDYFGR
                     PRTAPTLAGPFSQLEVGEVTLKVWPH"
     gene            complement(1439..2371)
                     /gene="ispH"
                     /locus_tag="PKI05_04630"
                     /gene_synonym="lytB"
     CDS             complement(1439..2371)
                     /gene="ispH"
                     /locus_tag="PKI05_04630"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOA51517.1"
                     /translation="MRILLAAPRGFCAGVNMAIETLERALRRYGPPLYVYHEIVHNRW
                     VVNEFVQRGVHFVESLDLVPNGGIVIFSAHGVSPEIRREAAARNLHVIDATCPLVTKV
                     HREVQRFAAMGYTILLIGHAGHDEIIGTMGEAPDQIRLIKDEEEALTVDVPDPEHVAY
                     TTQTTLSVMDAARIVECLRRRFPKLVGPARDDICYATQNRQEAVLELAREADVFLVVG
                     SQNSSNSRRLAEIAQRTGIPAYLVDGPEDLKKEWFSAEQTVGITAGASAPEHLVQGCI
                     EFLRAHFFATVEERVIRTENVHFQLPPELRQDQP"
     gene            2470..3453
                     /gene="hpnC"
                     /locus_tag="PKI05_04635"
     CDS             2470..3453
                     /gene="hpnC"
                     /locus_tag="PKI05_04635"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HOA51518.1"
                     /translation="MMASKQSRLSTISPPGSSRDRDRFFADLAQFGPTAQYQPLSLRA
                     SRRYCRQLARSHYENFTVVSLFVPRDLRQHFCNIYAWCRWADDLADETGDPRWGMDLL
                     QWWQQELLACYQGVMRHPVLVALHETVTRFNIPRDPFLDLLTAFRQDQRVFRYETFEE
                     LLDYCRHSANPVGRLILFLGRCHTPDRVMLSDQICTGLQLVNFWQDVGQDYDRGRIYI
                     PKAECRRVGYTEEMFARREFNSAFRTLMKGLVHEAAAFLRRGRPLVAMMPRKLQIPVY
                     LFVRGGLAIARAIESHDYDVWTHRPTLSRSKKVELMIRTVWDVLCRRYDRD"
     gene            3469..4443
                     /locus_tag="PKI05_04640"
     CDS             3469..4443
                     /locus_tag="PKI05_04640"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HOA51519.1"
                     /translation="MPRIDPPDSQLAVSYAFCRAICRAAGSSFYPAFSMLSQEKAQAM
                     ETIYAFCRFTDDLVDDKLSVDPAQDLETWYQWVHGALVRGDRSWGELHSDLGTSASPV
                     SRGRALLPALVDTVEQFRIPPESLLRVIDGVRSDLTPRHVATFAELEDYCSKVASAVG
                     VACLCVWGMETQSIPPEAHDCGVAFQLTNILRDLAEDVKLGRIYLPQEDFVRFRCSLE
                     DFAARRVTPQWLALLDFEIERARNYYRRAAHIHGLLAPDGRRIFGLMWDVYWQLLKEI
                     ARRKEQLLERRVRLSRLRKSLLWLRWMVFPPKKLIEPTSPQESGSVKG"
BASE COUNT          955 a         1276 c         1286 g         1019 t
ORIGIN      
        1 atggagaatg gcttgccgag gctgccagta aggaggacct tttcaagccc tcgtcgggtt
       61 tgcgatggtg gtttgccgag gttgatgagg aaaagaccac gatttgggcg cagttccccg
      121 gggtcgatcc gaaccgagaa accgtggaga tcaacgtccg gcaaaccgtc ttttatccac
      181 gaacgccagg gtgcaattac atcaccgttc gtggttttat catgcgtcat gcggccacac
      241 cctgggcacc gcccacagcc caacaaatcg ggctgatcgg tacgcactgg agcaaaggtt
      301 ggatcatcga gaacaacgtc attagccact ccatttgctg tggaatcaca cttggcaaat
      361 atggcgacga atttgataac acgtcagcca atacggccga gggctatgtg caaacgatca
      421 atcgggccct ggccaatggg tggcatcgag acaatattgg ccaccacatc gtccgtaaca
      481 acatcatttc ccattgcgaa caggccggga ttgtcggcag tttgggggcg gccttttgcg
      541 aagtgaccga caatgtgatc cacgacattc acatgcggcg actcttttcc ggggcggaaa
      601 tggccgggat caagttccat gctgccatcg atacgctgat tgcccgcaat cacattttcc
      661 gtacctgccg gggattgtgg cttgactgga tggcccaagg aacgcgggtc cacgccaatc
      721 tctttcatga caacgacgaa gacctgttcg tggaagtcaa ccatggtccc tttgtggtgg
      781 ataacaatct gttcctttcc aaactgaatc tgcgtgatat gtcacaaggt ggtgcctatc
      841 ttcacaatct gtttgcgggg gcgatcaccg tttgccccga acccaatcgc aaaacgccgt
      901 tccatccgcc acacaaaaca gaggtcgccg ggttggcgag catccagggt ggggacaatc
      961 ggttcctgaa caatgtcttt atcggtcggg gtgaaaacac gcccatgccg gagggggccc
     1021 ctgccccacg acggacctgg atcacaggat ttggactgtc agcctacacc gcccaggcca
     1081 tgatgaatct gaccgagggc aatatttacc tcgatcatgc gccacctgct caaaacgagg
     1141 caaactcctt agagatcaag gaagccccct tctgccggct catggccgat gagggtatgg
     1201 tttttctcac gctgacgacg tctgcggatt gggctaacgc ggggagggaa atcgtcacgg
     1261 ccgcccgact tggtcagaca gccgtctcgc agcttcctta tgagaactac gatgggcgga
     1321 acttggtcat cgatacagat tactttggtc ggccgcgaac agcccccaca ctagcaggcc
     1381 cgttttctca actagaagtg ggcgaagtca cgctcaaggt ctggccgcat tgatcgcctc
     1441 aaggctgatc ttggcgaagt tccgggggca actggaagtg gacgttttct gtgcggatga
     1501 cacgctcctc cacggtggcg aaaaaatgtg cacggagaaa ctcaatacat ccttgcacga
     1561 gatgttccgg ggcgctggcc cccgccgtga ttccgaccgt ttgttccgct gaaaaccact
     1621 cctttttaag atcctccgga ccatcaacaa gataggcggg gatacccgtc ctttgggcaa
     1681 tctcggccaa gcgacggctg ttggaactgt tttggcttcc cacgaccaag aacacgtcag
     1741 cttcccgggc caattccagg acggcctcct gacgattctg cgtggcatag catatgtcgt
     1801 cccttgccgg gccgacaagt ttaggaaacc gccgccgcag gcattccaca attcgtgcgg
     1861 catccatcac ggagagggtc gtttgggtgg tataggcaac atgttccggg tcgggtacat
     1921 caaccgttaa tgcctcttcc tcgtctttaa tgaggcgaat ctgatcaggg gcctcgccca
     1981 tagtaccgat aatttcatcg tgtccggcgt ggccaatgag taagatcgtg tatcccatgg
     2041 cggcaaatcg ctgcacctca cggtggacct tcgtcaccaa gggacacgtg gcgtcaatga
     2101 cgtgaagatt gcgggccgct gcttcgcggc gaatctcggg cgaaacaccg tgggccgaaa
     2161 agatcacaat tccaccgttg ggaaccaggt cgagggattc cacaaagtgc acccctcgct
     2221 ggacaaattc gttcaccacc cagcgattgt ggacaatctc gtgatacaca taaaggggtg
     2281 ggccataacg ccggagggcc ctctccaagg tttcaatcgc catattgacc ccggcgcaaa
     2341 atccgcgcgg agcagctagc aggattcgca tggttttctc aatgatcctt tcagaggata
     2401 atgatcacta acgcagggac tactgcggat ttttgcctgt tttgccgtta ttccgtttca
     2461 ctttgacgca tgatggcctc aaagcagtcc cgactttcga ccatttcgcc gcctggttca
     2521 tcgcgggacc gggatcgctt ttttgctgat ttggcacagt tcggcccgac ggcacaatat
     2581 cagccgcttt ccctgcgggc aagccgccgc tattgccgtc agcttgcccg aagccactat
     2641 gagaatttta ccgtcgtcag tctctttgtg ccgcgggatt tacggcagca cttttgcaat
     2701 atctacgcat ggtgccgctg ggcggacgat ttggcggacg aaacgggtga tccccgatgg
     2761 ggcatggacc tccttcagtg gtggcaacaa gagctcctgg cctgttatca gggagtcatg
     2821 cgacatcccg tgctggtggc cctgcacgag acggtaacac ggttcaatat tccgcgagac
     2881 ccatttctcg acctgctcac cgcctttcgc caagaccagc gggtattccg ctacgaaacg
     2941 tttgaagaac ttctggatta ctgccgccat tcggcgaacc cggtgggacg gctcatcctg
     3001 ttcttgggac gatgtcatac gccggatcgg gtgatgctgt cggaccagat ttgcacgggt
     3061 ttgcagttgg tcaacttttg gcaagacgtt ggccaggatt atgatcgcgg gcgaatttac
     3121 atcccgaagg cagagtgtcg gcgcgtcggt tacactgagg agatgtttgc ccggcgagaa
     3181 tttaactcag cctttcgcac gcttatgaaa gggctggtcc atgaggcggc tgccttcttg
     3241 cggagaggcc gccccttggt ggccatgatg ccgaggaaac tccagatccc cgtttacttg
     3301 ttcgtccgcg gtgggctagc catcgcccgg gctattgaaa gccacgatta tgatgtgtgg
     3361 acccaccgac ccacgttatc ccgaagcaag aaagtggagc ttatgattcg caccgtgtgg
     3421 gatgtccttt gtcgccgtta tgatcgagat tgaaggcgca gccattcgat gccacggatt
     3481 gatccacctg attcacagct tgccgtcagt tacgccttct gccgtgccat ctgtcgggcg
     3541 gcgggatcca gcttttatcc cgccttttcc atgctcagcc aggaaaaagc ccaggccatg
     3601 gagaccatct atgccttctg ccgtttcacg gacgacctgg tcgatgacaa gttgtccgtt
     3661 gatccggcgc aggacctgga aacgtggtac caatgggtcc acggggcgct tgtgcgtggg
     3721 gaccgctcgt ggggtgaact gcattcagac ctcggcacat cggcttcacc ggtttcccgg
     3781 ggacgtgcgc tacttccggc cctcgtcgat acggtggagc agtttcgcat ccctccggag
     3841 tcactgctgc gtgtcatcga cggtgtgcgc agcgatctaa cgccccgaca tgtggccaca
     3901 tttgccgaat tagaggacta ctgtagcaag gtggcctccg ctgtgggtgt ggcctgcttg
     3961 tgcgtctggg ggatggaaac acaaagtatc ccgcccgaag cccatgactg tggcgtggcc
     4021 tttcaattga cgaacatcct ccgcgacctg gcggaggacg taaagctagg acgcatctat
     4081 ctcccgcaag aggatttcgt ccgctttcgt tgttcgctgg aagacttcgc agcacggcgg
     4141 gtcacgccgc aatggttggc cctcttggat tttgagatcg aacgcgcccg caattattac
     4201 cgccgggcgg cgcacatcca cggactactg gcgcccgatg gccgtcggat tttcggcctg
     4261 atgtgggatg tctattggca actgctcaag gaaatcgccc gacgaaaaga gcaattactg
     4321 gaacggcgtg tgcgattatc tcggttgcgg aagtctcttc tctggttgcg ttggatggtc
     4381 ttcccgccga aaaaactcat tgagccaacg tcgccacagg aaagtggttc tgtgaaaggc
     4441 taactcccac cgcttgagga tgcaaccatg tcaactgggg aagctaccgg aggaaataca
     4501 aatcgcgaca cgcggcgggt ggcgatcgtc ggcgcc
//
LOCUS       DAOPTR010000174         4524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_15503_length_4524_cov_2.889684, whole genome shotgun sequence.
ACCESSION   DAOPTR010000174 DAOPTR010000000
VERSION     DAOPTR010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15503_length_4524_cov_2.889684"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PKI05_04645"
     CDS             complement(<1..313)
                     /locus_tag="PKI05_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51520.1"
                     /translation="MMPDNQRPQRFIDRLESAVRRRRTPVLVGIDPHLELLPPNVGAG
                     DVENRWMLPREKLAEAVRVFCAGVLDVVASLVPAVKFQLAFFEQLGPEGMRVLLELVA
                     RA"
     gene            complement(596..2980)
                     /gene="pheT"
                     /locus_tag="PKI05_04650"
     CDS             complement(596..2980)
                     /gene="pheT"
                     /locus_tag="PKI05_04650"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOA51521.1"
                     /translation="MKISLDWISDYVDINDIPVDVVADRLTMCTAEVEDVLEVRRSVA
                     QVVVGEVIDCRDIPAAPNLKLVTVDCGTRRFQTVCGAPNVRKGLKAPFAPPGTRLANG
                     VEIAVAEVSGHRSEGVLCSPAELGMSRWHEGLLECPPSLPNGAPLAQWIPDRDVLIEI
                     DNKSLTHRPDLWGHYGFARELAAIFRRPLRPLPVVDLSQYDHLPTYPLTVDDYENCPC
                     YGCIDLRVRAGVPAPLVIQRRLHALGQRTFNLMVDLTNYIMWEIGQPTHAFDGDRVHA
                     IRVAPLGKPGTFVTLDGQERQLLPDDLLIWNEKEPVALAGVMGGLESEVTPTTTHVLL
                     ESANFKASRIRRTSVRLDLRTEAAQRFEKGQPPVNVKIGTARALQCLIDSGEPFEVLS
                     RFTVAGDLKEAPRPLSIPWRTVDTMAGQKIPRQEAVGILRHLGFLAEEKGETLEVGIP
                     AHRSEKDISIPADVVEEILRIYGYDRIEPRLPEGPMAALPVNVGLRKEHQARRVLAGA
                     HGFVEVQNYVWLDDNWLAELGYQPGETLTLKNPPAQTCRRLRPVLLPNLLALVRPNRA
                     HRDMFRIFEIGRVFRPQANDGCEERTHLAGVSFRSSGKGDQEEHFRSIRGSLEDLGAV
                     LTGQALRFVAGQDSPFPWCVPGYWVKILLGDTTVGAAGVLDPVLARVVALEGQVVWFE
                     LDLEAIQAPIYPVVHYRPLAVYPRSWQDFSLLWDVTKGFDSLEAVLSKFQHPLISERE
                     FLYVFKGKGLPQGQASYTFRYWLAAPDHTLTGEEIEGFRTAFLSFLQENGISLR"
     gene            complement(3083..4108)
                     /gene="pheS"
                     /locus_tag="PKI05_04655"
     CDS             complement(3083..4108)
                     /gene="pheS"
                     /locus_tag="PKI05_04655"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOA51522.1"
                     /translation="MDEILEQLRRDVAAATDSKQVEQVRVKYLGRKGIITTLAKNTDF
                     SRLSSEEKRSFGQRLNALKSEAEQLLKEALEKAKAAEKDLVRKVAGLDLSLPGIDRRL
                     GSIHPIALVQMELEEIFQGMSFRVLTGYEVELEYYNFDALNIPPDHPARDMQDTFWLD
                     NGMLLRTHTSANQVRALETYGVPLRAIFPGRCFRNEAIDASHENTFYQLEGLLVDRDI
                     SVAHLIAVMKTLLSQVFKREVTVRLRPGFFPFVEPGFELDVQCLICGGKGCATCKQTG
                     WIELIPCGLVHPKVLEFGRVDPNIYSGFAFGMGLTRMAMMKFGIPDIRLFNSGDIRFY
                     EQFPAAF"
BASE COUNT         1015 a         1359 c         1222 g          928 t
ORIGIN      
        1 gcgctcgagc taccagttcc aaaaggaccc tcatcccctc cggacctaac tgctcaaaaa
       61 atgccaattg gaacttcacg gcggggacca gcgaggccac cacgtcgagg acccccgcac
      121 aaaagaccct gacggcctcg gcaagtttct cgcgaggaag catccaacgg ttctcaacat
      181 cgcccgcacc cacgttcggt ggaagaagtt ctagatgggg gtcgatcccc accagaaccg
      241 gcgtccgacg ccgccgaacg gctgactcaa ggcgatcgat gaatcgctga ggtctttgat
      301 tgtcaggcat catggctcct tccagcggac tttgtgggtc aaacttgttg ttttcccagc
      361 aatatccaag ttttgctagc aataccgaag caagttgcct atccgatgtg gaacggttta
      421 tcccattcta ttgacccgca ccatggccga acacatcggc cgacacctcc gatgacacga
      481 aggcggcaaa acccccgggg gttgtccgta gtcgtcttga cttggcattc atccatcgcc
      541 ccaattgccc catttagcaa gattcgggcc gcaaatcgcc acggtgaaat gggggttagc
      601 ggagcgaaat cccgttttct tggaggaagc tcaaaaaggc ggtccgaaac ccttcgatct
      661 cttcccctgt caacgtatgg tcgggggcag ccaaccagta gcggaacgta taacttgctt
      721 gaccttgagg gagtcccttc cctttgaaga cataaaggaa ttcccgctcg ctgataagcg
      781 gatgctggaa tttggaaagg accgcctcca gcgaatcgaa gcccttcgtc acgtcccaca
      841 acagggaaaa atcctgccac gaacggggat aaacggccaa cggtcggtag tgcaccacgg
      901 gataaattgg ggcttggatc gcttctagat ccagctcgaa ccacacgacc tggccttcca
      961 gggcgaccac cctggcaagg accgggtcga gaacgccggc ggcccccaca gttgtgtccc
     1021 ccaagagaat cttcacccaa tagcccggca cacaccacgg aaatggcgag tcctgccccg
     1081 caacgaaacg gagggcctgc ccggtcagga ccgcgccgag gtcttccagt gacccgcgaa
     1141 tgctgcggaa atgctcttct tgatcaccct ttcccgatga acgaaaactc accccggcca
     1201 ggtgggtccg ctcttcacat ccatcattgg cctggggccg aaatacccga ccaatctcaa
     1261 agatgcgaaa catatcccga tgggcccggt tgggtcgaac cagggccaat agattgggca
     1321 gcaacacagg gcggagtcgg cgacacgtct gggcaggagg attcttaagg gtcagtgttt
     1381 ctcccggctg ataacccaat tcagccagcc aattgtcatc gagccagaca tagttctgga
     1441 cctcgacaaa cccatgggca cctgcaagca cgcgccgggc ttgatgctct ttccgcagtc
     1501 ccacgttgac aggcagggcg gccatggggc cttcgggaag acgtggttcg attcgatcgt
     1561 agccataaat gcgaaggatt tcctccacca cgtcggccgg tattgaaatg tccttctcac
     1621 tccgatgggc aggaatccca acctccaggg tctcgccctt ttcctcggct aaaaaaccca
     1681 aatggcggag gatgcccacg gcctcttgcc gaggaatctt ttggccggcc atggtatcga
     1741 ccgtccgcca ggggatggac aaaggacgcg gtgcctcttt caagtccccc gccaccgtaa
     1801 accgggaaag gacctcgaac ggttctccgg aatcgatcaa gcactggagg gcccgggccg
     1861 tgccgatctt cacgttcacg ggcggttgtc ccttttcgaa tcgttgggcg gcctccgttc
     1921 gcaaatcgag acgaaccgag gtccgacgga tgcgggaggc cttaaagttt gcactctcca
     1981 gaaggacatg tgttgtggtg ggagtgacct cactttccag cccgcccatc acgcctgcca
     2041 aggccaccgg ctctttctca ttccaaatga gtaggtcatc gggcaggagt tgccgctctt
     2101 ggccgtccag cgtcacgaac gtccccggct ttccgagggg tgccacacgg atggcgtgaa
     2161 cacgatcgcc atcaaaggcg tgggtgggct gtccgatttc ccacatgatg taattggtca
     2221 gatcgaccat caggttgaag gtccgctgtc ccagggcatg gagtcgtcgc tggatcacaa
     2281 ggggggccgg cacacctgcc cgaacccgca agtcaatgca accataacag gggcagttct
     2341 catagtcatc caccgtcaat ggatacgtcg gcaaatggtc gtactgcgaa agatcgacga
     2401 ccggtaacgg ccgcaatggc cgacggaaga tcgcggccag ttcgcgggca aaaccgtagt
     2461 gcccccagag gtccggccgg tgggtcaggc tcttgttatc aatttcaatc aagacgtccc
     2521 gatcgggaat ccattgggcc aacggtgcgc cattcggtag cgacggtgga cattccaaaa
     2581 ggccctcgtg ccagcggctc atccccagtt ctgccggtga acagagaacc ccttcgctac
     2641 ggtgaccgct gacctcggcc acggcgattt ccacaccatt ggcaagccgg gtacccggcg
     2701 gggcaaatgg tgccttcaag cccttgcgaa cattcggcgc tccacaaacc gtttggaaac
     2761 ggcgcgtgcc acaatcgacg gtgacaagtt tcagattcgg cgccgccggg atatctcgac
     2821 aatcaataac ttcgccaaca acgacctgag ccacgctccg ccggacctca agaacgtctt
     2881 cgacctccgc cgtacacatg gtcagtcgat cggccactac atccacgggg atgtcgttga
     2941 tatcgacgta gtcgctaatc caatcgagag agatcttcac agctcactcc gtgacaccac
     3001 ttcactaatc gttttactgt cagtgattca aaaggcgttc gttttatctc gtgtgcgccc
     3061 cacacacagc cagaacccgg cttcagaatg ctgccgggaa ttgctcgtaa aagcggatgt
     3121 cgcccgagtt aaataagcga atgtcgggga tgccgaattt catcatggcc atacgggtca
     3181 gccccatccc gaaagcaaag ccactataga tgttgggatc gactcgccca aactcgagca
     3241 ctttgggatg gaccaggccg caggggatca attcgatcca cccggtctgt ttgcaggtgg
     3301 cacatccctt accgccgcaa atgaggcatt gaacgtccaa ttcgaagccc ggctcgacaa
     3361 acgggaaaaa gccgggacga agccgcactg tcacttctcg cttaaaaacc tggcttaaca
     3421 atgtcttcat tacggcgatg aggtgagcca ccgaaatatc acggtccacc aggagacctt
     3481 ccaactggta aaaggtattc tcgtgactgg cgtcgatcgc ctcattgcgg aagcagcgtc
     3541 ctgggaaaat cgcccgcaac gggacgccgt acgtctctaa cgcccgcacc tggttggccg
     3601 aggtgtgcgt ccgcaagagc atcccgttat cgagccagaa cgtatcctgc atgtcccgag
     3661 ccgggtgatc tggcggtata ttgagggcat cgaagttgta gtactccaac tccacttcat
     3721 agccggtcaa cacgcggaag ctcatgccct ggaaaatctc ttccagttcc atctgaacca
     3781 gggcaatggg gtgaatactt cccagccggc gatcaatacc gggaagggaa agatccaagc
     3841 ctgcgacctt gcggacgagg tccttttctg cggccttggc cttttccagg gcctctttta
     3901 gcagttgctc cgcctcgctt ttgagggcgt tcaatctctg cccaaaggac cgcttttctt
     3961 ctgatgaaag tcggctgaag tctgtgttct tggccaaggt ggtgatgatt ccctttcggc
     4021 cgagatactt gacccggacc tgttccactt gctttgagtc tgtggctgct gccacatcac
     4081 ggcgaagttg ctcaaggatt tcgtccatac cagtaagtca atcgtttaaa ttccaaagtt
     4141 agatatgttg ggttgggatg cgctggtgat gcctcgttac tgggccgcca attccctacc
     4201 ggtgcccgcg caatgagcca ccctgcccga cgaatgatca agaaatgccc agtttatcgc
     4261 gcgacgtcaa tgttgggtat ctgggtttgg tcgcaaaagt ggcccacttt ccctcacaac
     4321 gttggcagaa gagcaacatc cgttgtccaa aatgcgcatc actcatcata atagcgggtg
     4381 gcaaagggtg ataagagcct atcccataat ctcccctcgc ccgttcggcg ggagaggggc
     4441 agggggttat ggtggctcga atcgctgccg gtgttctcga tcgactccga cggtcgcttg
     4501 ccctcacccc aaccctctcc caga
//
LOCUS       DAOPTR010000175         4513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_15548_length_4513_cov_3.709735, whole genome shotgun sequence.
ACCESSION   DAOPTR010000175 DAOPTR010000000
VERSION     DAOPTR010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15548_length_4513_cov_3.709735"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PKI05_04660"
     CDS             <1..629
                     /locus_tag="PKI05_04660"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOA51523.1"
                     /translation="ALAEIEVNISYCTITSPRNGRIVDRLAEEGDTASPGVPLLTLYD
                     PASLRLEVPVIENVAAHLNIGDKVKVYIDALQQEIEGTVDEKVPQADVATRTVLVKVS
                     LPETPGLVEGMFGRLKIEAGSREHLCLNIQAIKRIGQLDFVEVVREDGTKEQRLIQIG
                     RFGRPGHVEVLSGLNAGEKVAIPKRPETSESIGTDQPQCLASPASEQQ"
     gene            808..4230
                     /locus_tag="PKI05_04665"
     CDS             808..4230
                     /locus_tag="PKI05_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOA51524.1"
                     /translation="MTQSTERVPFLTRIVDVFLRGDSAIMLTVISVLIGLGALWITPR
                     EEEPQIVVPMADIFVSAPGLSAQEVEQQITNRLERIVYQIDGVEYVYSMSRPETAIVT
                     VRFYVGQDREDSLVKLHAKVQSYIDEAPSSVKGWVIKPVEIDDVPIVNITLWSRDPEH
                     AHDYELRRIAEQLQNELKSIPQTNRVTIVGGRPRHIRVEVDPIKLAARSTSILQVAQA
                     LKVSNVNVRAGRYEQRNREFLVEAGTFVRDARELENLVVNVVGNRPVYLKDVAQVIDG
                     PAEPVSYTWMGFGAAAKHPELPREHVTAEELKSRSNAIPLQYFPAVTIAVAKQKGTNA
                     VWVAQAVEERIAQLAKTHLPSNVFYRITRDYGETANEKVNELVEALIVAVLTVIGLIW
                     LFVGWRAAVVIALAVPVCYSVTLFINYLAGYTINRVTLFALILALGLLVDDPITDVEN
                     IARYFAMRILPPRPAVLRAVQEVRPALVMSTLAIVASFAPMAFITGMMGPYMAPMALN
                     VPVTLMVSLIVSLVITPWMAMFALQGSKEHGDSTEQGYDLTKRPIYRMVRAVFHPILN
                     YRATAWGVVLAVAILFVASLLLPGLRVVPLKMLPYDNKNEFQVVIDMPEGTTLEQTDA
                     AARRIAAYLATLPEVRDFQIYAGLSSPMDFNGLVRHYFLRQGSHLADIRVNLLHKDFR
                     AQQSHEIILRIRADIAKIAQEINANIKLVEVPPGPPVISTITAEVYGPPDASYEDLIR
                     AAKFVEARMQREPGVVDVDISAEADQEKRLFVTDQPKAALSGVSKEMIAQTVQAALGG
                     YPATELHIPSEVNPLPVILRLKRSERSAEDDLAELYVPGSQGQLLQLGSLGKFVSTSP
                     EGKPLLEDKTIYHKNLRRVVYVFGEVAGRPPADAIIDMQVDLVPENEFPRKKPRPRPV
                     EARTWFHPGGNDFWTMPEGYQAVWTGEGEWKITLDVFRDLGIAFGVALVVIFILLMFE
                     TKSRVLPLIIMLAIPLTMIGIMPGFWILNLITNRPVGGFANPVFFTATAMIGMIGLAG
                     IVVRNSVVLIDFIHHSLLDGFDLKEAIVRSVAVRFWPIILTSGTTLLGNWVITLDPIF
                     SGLAWAIIFGIFASTTFTLVVIPTVYWLLYAKRGGAIAVPSAKRETPSEQVA"
     gene            complement(4274..>4513)
                     /locus_tag="PKI05_04670"
     CDS             complement(4274..>4513)
                     /locus_tag="PKI05_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51525.1"
                     /translation="EIEDLVRLASQPDKYVVLIAGPCSECHRLKTDALLPLIQEPNLR
                     LWTHLVMDLDTARELLTHPKHAISQNVIVPDKGGP"
BASE COUNT         1012 a         1206 c         1239 g         1056 t
ORIGIN      
        1 aggccctggc cgagatcgag gtcaatatct cctactgcac gatcacctca ccgagaaatg
       61 gccggattgt tgatcgtttg gccgaagagg gtgacacagc gtcaccgggt gtgccgctct
      121 tgacgcttta cgaccccgcc tcgctccggc tggaggtccc tgtgattgag aacgtagccg
      181 cacatttgaa catcggtgac aaagtgaagg tctatatcga cgcccttcaa caggagatag
      241 aagggactgt ggatgaaaaa gtcccccaag ctgatgtggc caccagaact gtcttggtca
      301 aggtttcgct gccggagacg ccaggcctcg tggaaggtat gtttggacgg ctcaagatag
      361 aagccggctc gcgcgaacat ctctgcctca atattcaggc gatcaaacgg attgggcagt
      421 tggactttgt ggaagtggtc agagaagacg gcacgaaaga gcagcgtctt atccagatcg
      481 ggcgatttgg tcgcccagga cacgtcgagg ttttaagtgg gctaaatgct ggcgaaaaag
      541 tggctatccc caagaggccc gagacaagcg aatccatcgg gacagaccag ccgcaatgtt
      601 tggcgtcacc tgcttcagaa caacagtaag gtgtgtgtca gtggcagcga attttggttt
      661 tggtgatgat tgttcttcgg agcctcgctt gtccaaaaaa gtgacaccca cagagaaaat
      721 cacgatagag caggaccgcg tgaacacttc agggcttacg ctgataagca gccaacaaac
      781 catgaaaaga ataacggagg gcgtgacgtg actcaatcga cagagcgagt accctttttg
      841 acacggatcg tcgatgtatt tctccgtggc gattccgcga tcatgctcac cgtcatttct
      901 gtcctcatcg gcctgggtgc tttgtggatc accccgcgtg aggaagaacc ccagatcgtc
      961 gtacctatgg cggacatctt tgtctcagcg ccaggacttt cggcacaaga ggtcgaacag
     1021 cagatcacaa atcggctgga acgaatcgtc tatcaaattg atggcgttga gtatgtctat
     1081 tccatgtcgc gtccggaaac ggcgatcgtc acggtgcggt tctacgtggg tcaagaccga
     1141 gaggattcgc tcgtcaaact ccacgcaaaa gtgcaatcgt acatcgacga agcgccgtcc
     1201 tctgtcaagg gttgggtcat caagcccgtg gagatcgatg acgtcccgat tgtgaacatc
     1261 accctatggt cccgcgaccc cgagcacgct catgactacg agttacgacg gattgccgag
     1321 caactgcaga acgagctgaa atcaattccg cagaccaacc gtgtgaccat tgtaggcggt
     1381 cgtcccagac acatccgtgt tgaggttgac cccatcaaac tggcggccag gagcacgtcc
     1441 attctgcaag tggctcaagc cctgaaggtg agtaacgtca acgtcagggc tgggcggtac
     1501 gaacagcgaa accgtgaatt cctcgttgaa gccggaacgt tcgtgcgcga tgcccgggaa
     1561 ctggaaaacc tcgttgtcaa tgtggtgggc aaccgacccg tttatctgaa agacgttgcc
     1621 caagtcatcg acggaccggc tgagcctgtt tcgtacactt ggatgggatt tggggcggct
     1681 gccaaacatc cggagttgcc ccgggagcac gtaacagccg aggagttaaa atccaggtcg
     1741 aacgccatcc cactgcagta cttccccgcg gtgactattg ccgtggccaa acaaaagggc
     1801 acaaatgccg tctgggtcgc tcaggcagtg gaagaacgca tagcccagtt ggcgaaaaca
     1861 catttgccat cgaatgtttt ctatcgcatc acacgggatt acggagaaac agcgaacgaa
     1921 aaagtcaatg agcttgtgga agctctcatc gtcgccgttt tgactgtgat tggcctcatt
     1981 tggcttttcg tcggatggcg ggccgcggtc gtcattgccc tggcagtgcc cgtgtgttat
     2041 agcgtcacat tgtttatcaa ttatctggcg ggttacacaa tcaatcgggt gacgctcttt
     2101 gccctcattt tggcattggg gcttctcgtg gatgatccga tcacggacgt ggaaaacatc
     2161 gcccgctatt tcgccatgcg gattttgccg ccccggcctg ccgtcctccg cgctgttcaa
     2221 gaggttcgcc ccgccttggt gatgtcaacc ctggccatcg tggcaagctt tgccccgatg
     2281 gcgtttatca cgggaatgat ggggccttac atggccccta tggcgctcaa tgtccccgtg
     2341 acgctcatgg tgtcgctgat tgtgtccctg gtcattactc cgtggatggc catgttcgcc
     2401 ctgcaaggtt ccaaagaaca cggagacagt actgaacaag gctacgattt gaccaaacgg
     2461 ccgatttacc ggatggttcg agcagtcttt catcctatct tgaattatcg agccacggcg
     2521 tggggggtgg tcctcgccgt tgcgattctc tttgttgcgt cactcttgct accgggcttg
     2581 cgggtcgttc ccctaaaaat gctcccctat gataacaaga atgagtttca ggtcgttatt
     2641 gacatgcctg aaggaacgac tctggagcag acggacgcgg ctgcccgacg tatcgcggcc
     2701 tatcttgcta ctttgcctga agttcgagat tttcagattt acgccggctt gtcctcgcct
     2761 atggacttca atggtctggt cagacactat ttcctgcgac aaggaagtca cctcgccgat
     2821 attcgggtca atttgctaca caaggatttt cgtgctcaac aatcacacga aattatcctc
     2881 agaatccgcg ccgacatcgc aaagatcgca caagaaatca acgccaatat taagctcgtc
     2941 gaagttccgc ctgggccgcc tgtgatttcg acaattacgg cggaggttta cggtccaccc
     3001 gacgcttctt acgaggacct tatccgcgcg gccaagtttg tcgaagcacg gatgcaacgg
     3061 gagcccggag ttgtcgatgt tgacatcagt gccgaagccg atcaggaaaa gaggctcttt
     3121 gtcacggatc agcccaaagc ggctctttcc ggcgtctcta aggagatgat tgcccagact
     3181 gttcaagcgg cattgggcgg ttatccggcc accgaactgc acattcctag tgaggtcaat
     3241 cccctgcccg tcatcctgag actgaaacgc tcagagcgtt cagctgaaga tgacttggct
     3301 gaactctatg tccccggttc tcaaggacaa ttgctacagc ttggatctct aggaaagttt
     3361 gtttcaacca gcccagaagg aaagccactg ttggaagata agacaattta tcataagaac
     3421 ttgcgacggg tcgtttacgt cttcggcgag gtcgcaggcc gccccccggc cgatgccatc
     3481 atcgatatgc aggtcgattt ggtgccggaa aatgaattcc cccgtaaaaa gcctcggccg
     3541 cgaccggtcg aggcacgtac gtggtttcac cctggcggga acgacttctg gacaatgccg
     3601 gaaggatatc aggcagtttg gacgggagaa ggcgaatgga agatcaccct cgatgtcttc
     3661 cgcgacttgg ggatcgcctt cggggtcgcc ttagtcgtga tcttcatcct tctgatgttc
     3721 gaaaccaaat cacgggtcct ccccctcatc atcatgctag cgattccact aacgatgatc
     3781 ggcattatgc cgggattttg gattctgaat ctcatcacaa accggcccgt agggggcttc
     3841 gccaaccccg tctttttcac agcaaccgcc atgatcggca tgatcggtct ggcgggaatc
     3901 gtggtgcgca actcagtggt tctgattgac tttattcacc acagtttgtt ggatggtttt
     3961 gatctgaagg aggccattgt cagaagcgtt gccgtgcgat tctggccaat catcttgacc
     4021 tcgggcacaa cccttttggg gaactgggtg atcacgctcg accccatctt ctcgggtttg
     4081 gcctgggcca tcatctttgg gatattcgct tccactacct ttaccttggt ggtcattccc
     4141 accgtttatt ggttactgta cgcgaagcgt ggcggcgcca tcgcggttcc gtccgcaaaa
     4201 cgagaaacgc cttcggagca ggttgcctga ttccgattgt tcggtgaccg cggtagcgag
     4261 tcgccgggat tgttcagggc ccgcctttat caggtacaat cacgttctgc gaaatcgcat
     4321 gcttaggatg ggtgagtaac tcccgtgcgg tatcgagatc cataacgagg tgggtccaca
     4381 accggagatt gggctcttgg atcaaaggta acagggcatc agtcttcaaa cgatggcatt
     4441 cgctgcaagg tccggcaatt aggacaacat atttatccgg ctggctcgct agcctgacta
     4501 agtcctcgat ttc
//
LOCUS       DAOPTR010000176         4510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_15562_length_4510_cov_3.763187, whole genome shotgun sequence.
ACCESSION   DAOPTR010000176 DAOPTR010000000
VERSION     DAOPTR010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15562_length_4510_cov_3.763187"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(466..3477)
                     /locus_tag="PKI05_04675"
     CDS             complement(466..3477)
                     /locus_tag="PKI05_04675"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOA51526.1"
                     /translation="MPLRMMTIVAGLAILWTVLAVAGDPGSLPPGFRNAPGGGPLSNQ
                     NGGLSEGPAPADEGGVEEELVVEVRVVGAKNLPREKYLPSIKTRAGRPFDVNLLEEDV
                     RRLYRTGLFVDIKTYTPKVPGGRLVVFEVLERPLLHYVKFAGQRAFSEKRLAKEAGLK
                     VGDPLDTWQVDEAQRKLEKFYHEKGFSNARVTVIEGNKTTDRGAIFLINEGRRQRVAW
                     TGFVGNTIATDARLRTQVQTKPGWFWFLGGLFNRQQLDDDVERLTAYYRGLGFFRARI
                     GRNIEVYRDPLDDRREWVYVTYVIDEGPRYKVRDIRFFGNQKFSSEELTRILELKPGD
                     YFDQNKLQTDLNKMREKYGSIGHVFADIEPDPRFLEEPGELDLVYRIEEGHPYRVGMI
                     NVRIKGESPHTQVTTVLNRMSLAPGDIADIREIRASERRLRASQIFEVDPSRGAQPKI
                     AFSPPEIPVIAEESQGPRIAERPDRVVPASGTSPAQPEPSSNTTPSPDVFRGQDSGGG
                     ITLPSPPGWVFPNPFGRKPDTASSSSVIPAVGTMPSASAPQNVYGAVYQAPPNRSSNT
                     TLVANESTDVVTFPGGGGNVAVGAGEVTISDMNRGASPTSATVPQMAPDAQGRLVFPD
                     RPMPGDASVFSDRPPDEIPLELPLEPQVEEARTGRLLFSVGVNSDAGVMGSAIIEEQN
                     FDWTRWPRSWRDIIEGRAWRGAGQRFRLEAVPGTRVHRYMVSFSDPYFMNRNVTFGLS
                     GFYYRRDYYEYLEERIGGRVVFGYQFTHDLSGTVAYRGSDIKISDPISTPWGIPPTIN
                     AAVGTNILHGFEIALTHDTRDNSFLPTEGHFFQIAFEQVIGTYDYPRVELDYRRYFAL
                     RQRPDGSGRHVLGLASRFAVTGDETPVYDHYFAGGTSSLRGFDYRGASPREYGIAVGG
                     HCLLLASAEYMFPITADDMVRGVIFCDTGTVEPTIDSWDQRYRVSLGFGLRLVIPAMG
                     PAPIAFDFGFPISEEPGDDNRVFNFFIGFLR"
     gene            complement(3679..4059)
                     /locus_tag="PKI05_04680"
     CDS             complement(3679..4059)
                     /locus_tag="PKI05_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOA51527.1"
                     /translation="MSDSTTKKPLGEFRHIDAYDVDDVTVVHFKESRITDDLGIQELG
                     QELFSLVDGANRTKLLLNFANVQFLSSGALGKLRALENRVSKRGGVVKLCSINPNIYE
                     VFKITRFDTIFEIFQTEADALASF"
BASE COUNT          983 a         1334 c         1242 g          951 t
ORIGIN      
        1 cgcccgttcg gcgggagagg ggtcgggggt gacggtgcct cgaatcgctg cgggttttct
       61 caatcgattc cgacgaccgc ttaccctcac cccaaccctc tcccgaaggg agaggcgact
      121 ttttgggatg ggctctttgt actacgtttc cactcctatt ttgttgtttt tccttatcac
      181 cgtatcgtat taaattgcgg ccaggagaaa agggggttgg aaagcccaga aattcgtaga
      241 cgatttatct ctgctccagc agtcccgcga cctcagagat acggcatcgc ggttcgactg
      301 aagcgagtct gtcgattatc atccccccac cttcccctga gtttgtagtg gacatcacgc
      361 gggttttggg ctggtacccc tctcataaaa aatgacagcg gtctttgtcg tctcaaagtc
      421 gaggcaacga cgggagtttt ccggtggaag tggactgacc gctttttacc gcaagaagcc
      481 gatgaagaag ttgaacacgc ggttgtcgtc gccgggttct tcgctgatcg ggaagccgaa
      541 gtcaaaggca atcggggccg gtcccatcgc cgggatgacc agccgcaacc caaaacccag
      601 ggatacccga tatcgctggt cccaagagtc aatggttggc tcgaccgtac cggtgtcgca
      661 gaagatcacc ccgcggacca tatcatcggc tgtgatgggg aacatgtact ccgccgaggc
      721 caaaaggagg cagtgtccgc caaccgcgat accatattct cggggggacg ccccgcgata
      781 atcgaagcct cggaggctcg acgtaccccc ggcaaaatag tggtcgtaca ccggtgtttc
      841 gtctcctgtg acggcaaagc ggctggccaa gccaaggacg tgccgtcccg acccatccgg
      901 gcgttgccgc agggcaaaat agcggcgata gtccaattcc acacgggggt aatcgtatgt
      961 gccaattacc tgttcaaagg cgatctggaa aaagtgacct tcggtaggca ggaacgagtt
     1021 gtcgcgcgtg tcgtgcgtca aggcaatttc gaacccgtga aggatgttcg ttcccacggc
     1081 cgcgttgatc gttggaggga ttccccaggg cgtagaaatc gggtcagaaa tcttgatatc
     1141 cgagccgcgg taagcaacgg ttcccgaaag gtcgtgtgta aattgatagc cgaagacgac
     1201 ccggccaccg atccgctctt cgagatactc ataatagtct cggcgatagt aaaaaccact
     1261 caaaccaaaa gtcacatttc gattcatgaa ataggggtct gaaaagctga ccatgtagcg
     1321 gtgaaccctg gtgccgggga cggcttccaa ccggaaccgc tgaccggcac cccgccaggc
     1381 cctgccttca ataatgtctc gccagctccg tggccaccgc gtccaatcaa agttctgttc
     1441 ctcgatgatc gcgctgccca taaccccggc atccgagttg acccccacac tgaacaacaa
     1501 ccggccggtt cgggcctctt ccacttgcgg ctccaagggc agctcgagcg gaatttcgtc
     1561 cggtggccgg tcggaaaaca ccgaggcatc ccccggcata gggcgatcgg gaaacacgag
     1621 acggccttgc gcgtccggtg ccatttgtgg gactgttgcc gacgtgggcg aggcgccccg
     1681 attcatgtcg gaaatcgtga cctcgccagc ccccacagct acgttgcctc cacccccggg
     1741 gaaagtgacc acgtcggtgc tttcgttggc taccagggtg gtattggatg aacggtttgg
     1801 cggcgcttga taaacagcac cgtaaacatt ctgcggtgcc gatgcggatg gcattgtacc
     1861 aacggccgga ataaccgacg aagaactggc ggtatcgggt ttacgtccaa aaggattggg
     1921 aaagacccat ccggggggtg agggcagtgt gatacccccg ccactgtcct ggccccgaaa
     1981 aacgtctggc gagggcgtgg tgttggaact cggttccggc tgcgcaggcg acgttcccga
     2041 ggcaggcacg acgcgatccg gacgttcggc gatccgcggc ccttgggact cctcggcaat
     2101 cacagggatt tcaggtggac tgaaggcgat cttaggctgt gcgccccgtg agggatccac
     2161 ctcaaaaatt tgagatgccc gcaaccgccg ttcgctcgcg cgaatctcgc ggatatccgc
     2221 aatatcgccc ggggccagtg acatccgatt gagcacggtc gtgacctggg tgtggggaga
     2281 ttcccctttg atccgcacgt ttatcattcc gactcggtag gggtgcccct cctcgattcg
     2341 gtacaccagg tcgagttcgc ccggctcctc gagaaaccgc ggatcaggtt caatatcagc
     2401 gaagacgtgg ccgatcgacc cgtatttttc ccgcatctta ttgaggtcag tctggagttt
     2461 attttgatcg aagtaatccc caggcttcag ctccaagatg cgggtcagtt cttcactgga
     2521 aaacttttgg tttccaaaaa agcggatgtc ccgcaccttg tagcgaggac cttcatcgat
     2581 gacatacgtc acatagaccc actcccggcg gtcatccagc gggtcgcgat aaacttcgat
     2641 attacgccca atcctcgccc ggaaaaaccc taaaccacgg tagtaggccg tgaggcgttc
     2701 cacatcgtca tcaagctgtt ggcgattgaa caatccgccc aagaaccaaa accaaccggg
     2761 tttggtctgg acctgggttc tcagtcgggc atccgtggcg atggtgtttc ccacaaagcc
     2821 tgtccaagcc acgcgctgcc gccgaccttc attgattaag aaaatggccc cgcggtcagt
     2881 ggtcttattc ccttcgatca ccgtgaccct cgcgttggag aagcccttct cgtgataaaa
     2941 cttctccaat tttcgctggg cttcgtcaac ctgccaggtg tcgagcgggt caccgacctt
     3001 caggccggcc tccttggcca gccgcttttc gctgaaggct cgctggccgg caaacttcac
     3061 atagtgcaga agtggtcttt ccaagacttc gaacacaact agccgacccc cgggcacctt
     3121 tggcgtgtag gtcttgatat ccacgaaaag cccggtgcga taaaggcggc ggacatcttc
     3181 ttccagaagg ttgacatcaa atggccgtcc cgcacgggtc ttgatggacg gcagatactt
     3241 ttcccgcggc agatttttgg cccccacgac ccgcacctca acgacaagtt cttcctcgac
     3301 cccgccttcg tccgccggcg ccggcccctc ggagaggcca ccgttttgat tcgaaagagg
     3361 cccccctccg ggagcgtttc ggaaacccgg cggcagcgag cctgggtccc ccgcaacggc
     3421 cagcaccgtc cagaggattg ccagcccggc aacgattgtc atcatgcgta acggcataag
     3481 gcagagatcg tgcgctttct tgtcccggcg gcaatggatc gtggttaaac acgcggcgca
     3541 aagtaaaggg cgccgccccg cggaacaatt acccgatatt ggggtgagtg acaagacgag
     3601 tttcgcgaag aagcgactat ccggcgggaa aaggccacag cctgcttggg ggagaatcaa
     3661 cacttactgc caatttcctc agaaactggc gagggcgtcc gcctctgttt ggaagatttc
     3721 gaaaattgtg tcgaagcggg taattttgaa gacctcgtaa atgttgggat tgatcgaaca
     3781 aagcttgaca accccacccc gcttggaaac gcgattctct aaagcccgca gctttcccaa
     3841 agcaccgctt gacagaaact gcacattggc aaaattcagc aagagttttg ttcgattggc
     3901 tccgtccacc aacgagaaca actcttggcc caactcctgg atgcccaggt cgtcggtgat
     3961 ccggctttcc ttgaaatgaa cgaccgtcac atcgtccaca tcgtacgcat cgatatggcg
     4021 aaactcaccc agcggcttct ttgtagtcga atctgacatg gttgatcctc gtctcgacga
     4081 taagccctgc cggtcaccga ggtccacccg gttcccccga aacccgccct gtcgtcgttc
     4141 gcagaggtca cagaaaacca gtgcgacgat tttttatttt ttcccgggcg agcctgcctc
     4201 acatgacact tgcgccatca tactgtcaaa atcccttaat tcaagtggcc ctactttttc
     4261 ccgcggatcg tcaccccggt aacaccgcgt tcctctcgcc ggattactag aggcccacgt
     4321 gccattgtcc ctgggaacga caaccgatta caaatcggct cttcagcgga agagacggcg
     4381 cacgtccttc cgactttggc agtacacggc agccgtcacg tgagtttaga gcctatctca
     4441 aaaagtcccc tctctctccg ggagagggtt ggggtgaggg caagcgaacg tcggaatcaa
     4501 ttgagaaaac
//
LOCUS       DAOPTR010000177         4498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_15608_length_4498_cov_6.172631, whole genome shotgun sequence.
ACCESSION   DAOPTR010000177 DAOPTR010000000
VERSION     DAOPTR010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15608_length_4498_cov_6.172631"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..722)
                     /locus_tag="PKI05_04685"
     CDS             complement(132..722)
                     /locus_tag="PKI05_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51528.1"
                     /translation="MSVAGEVRVPYRIDARTRDLLTTNEHPTLAKMVNAAKEHVNHRP
                     GGVFYINEFSEVIVPGRHSGSCVYAGHYNGLLEFALDGEVVSTRAPAHLRPGNPWPGP
                     HVGVAYLLCAGGDDIKYELRQGNRTTLVYLSDVVGIDKAKKTAARVRAVKGSSGGKFY
                     INEQCEMFAPVAAESGWVFLYIGHLEDSAWFEPPEG"
     gene            complement(776..2824)
                     /locus_tag="PKI05_04690"
     CDS             complement(776..2824)
                     /locus_tag="PKI05_04690"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOA51529.1"
                     /translation="MVGFTAIGFDLYPWQSLAVDAWCHGVEGRPYTGTLEIVTGGGKT
                     LIAISCAARAAEMDRDIRLAIVVPTEALARQWKRNLLTYTSLDENEVGLLGAGGKATL
                     QDKLAIVAVVNTASKRLPPMSDSAQPLMLIVDECHRAGAPKYREVLKTRAKYRLGLSA
                     TPDREELDEDGEPLAYDEQAVGQSLGGVVYRFSLKDAREAGWLPEFTLHHHGVSLAAD
                     ERSEYETLSRRVDDAGDALRGCGFEPGRARSLVGRSDEVGEAARIWVQLTTQRKDLLY
                     RARERGRVAQLLTERLFKGDLRPRVILFHERVQEAVELHDALAADLPDIRVELEHSKL
                     PNRRREEALRAFADGDAPVLVSVKSLIEGIDVPQADTGISVASSSAVRQRVQALGRVL
                     RRAVSEDGVAKVSTMHLIYVDDTVDDLIYAKADWTDLTGADSNRYWKWPHGADQAEEV
                     ESPPRTPLPTEEQAWDLIGRQVEQEPVAWPGIAAGQEYSVKTNGAVFNAFGAQIANTQ
                     GVDQLVARVRGREGGRFRVTPQLGLILVWNPEAEKPSFYVVGQLQESFRVIPEVDAAT
                     AAEKVDELVAGDDYPGPADKKGGTFKVSQRGGGVIERRVSGGSEIARSDGTDASAQVA
                     NAREILKAWEKAGRSFSRFFVNSLRHAWYEDATGRKFLRQVDAGFAWPDEEESGGGGQ
                     "
     gene            2994..4466
                     /locus_tag="PKI05_04695"
     CDS             2994..4466
                     /locus_tag="PKI05_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51530.1"
                     /translation="MESDQKDVATLPAIAFEQRPACPRMFVTAARASEILEWADAPRA
                     SVDLMALYQRALDPKRAPELSQFIKSDKMNIVPGAVIIATAPGSLSASPSLNAQSSSG
                     PTACTVTISMVTPTREVALRNAIEQLNSRLSASELNEVKELEGDEEQEKEQLEQFEEE
                     AGDDEDSGLPSSYLTIVAADLEEAARDWDSLEHKKRAAIEEYLRYAAKPGMIIDGQHR
                     VYAAKNVNEFDVILPIVLIDALPMAEQVFHFYVLNNKAKPLTKTDLRRTISTALTATE
                     IDTLWSRLKEAGVDPDKVRLTHLINTDDNSPFKGLVDFGLAGDTGFIKENVAYQLVDN
                     FVSMPARYRALGEGVPAWDRKGDKIDRLPTFYAFWHAIMNSYPNAWSDGVEARGKCQF
                     FMKVSMLTLQSFVLDNLLTINNVLRRQDQPPPFEDLNALGDHVVSTIEDLPEEFFMRE
                     WTEKQMDTSQGRRILRAAIQEVIDARGKNLGNRSLFKTKA"
BASE COUNT         1088 a         1373 c         1112 g          925 t
ORIGIN      
        1 cctacgtgtg gaccgccacc gccgaatcca tcctcgccaa agtccagcgc gcacgcacca
       61 aactccaaca tgtagttagc caaaaatgag acggaccact agcaccgggc cagccacctg
      121 aagaaggtcg ttcacccctc cggaggctca aaccaagcgc tgtcttcgag gtggccgata
      181 taaagaaaaa cccaaccact ctccgcggca acgggagcga acatctcaca ctgctcgttg
      241 atgtagaact tcccaccgga tgacccttta acggctctca cccgtgccgc agtcttcttt
      301 gctttgtcta tacccacaac atcagacagg tagacgagag tggtccgatt gccttggcga
      361 agttcgtact tgatgtcgtc tccacccgca caaagcagat acgcgacacc gacatgcggt
      421 cccggccaag ggttgccagg acgaagatgg gcaggggccc gcgtgctgac aacttcgccg
      481 tccaaggcaa actcaagcaa tccgttgtaa tgcccggcat acacacaact tcccgagtgc
      541 cgccctggca caatgacttc actgaactca ttgatgtaaa aaaccccgcc tgggcgatgg
      601 ttcacatgct ctttggccgc attcaccatc ttggccaatg tgggatgttc gttggtggtg
      661 agaagatccc gagtccgcgc atcgatccgg tagggaactc tgacttcgcc tgccaccgac
      721 attgagtact tcgcatcctt ggcaatattt gaaggacaaa gtcccctata ctgtctcact
      781 gtccacctcc cccgctttcc tcttcgtctg gccaggcgaa gcctgcatcg acttgcctca
      841 ggaactttcg ccccgtcgca tcctcgtacc atgcatggcg aagagagttg acgaaaaacc
      901 gggaaaaaga gcggccagcc ttctcccaag ccttgaggat ctcccgggcg ttagcaactt
      961 gtgctgaggc atctgttccg tcagatcggg cgatctccga tcctccggac accctgcgtt
     1021 cgatcacgcc gccacctcgt tggcttacct tgaaagtgcc acccttcttg tcagctgggc
     1081 cagggtaatc gtcaccggcg actaattcgt caactttctc ggcggcagtc gcagcgtcaa
     1141 cctcaggaat gaccctgaac gactcctgaa gttgaccgac aacatagaaa ctgggctttt
     1201 ccgcctcggg attccatacc agaatcagcc cgagttgcgg cgtgactcta aaacggccac
     1261 cctcgcggcc ccgcacgcga gcaaccagtt ggtcgacacc ctgtgtgttc gcaatctggg
     1321 cgccaaatgc attgaaaact gcaccgttcg tcttcaccga gtattcctgt ccagcagcaa
     1381 taccgggcca ggcaacaggt tcttgctcta cctgtcgacc aatcagatcc caggcctgtt
     1441 cttctgtagg gagcggggtt cgtggtggag attccacttc ctcagcctgg tcagcaccat
     1501 ggggccactt ccagtatcgg ttggaatcag cccccgttag gtcagtccag tcggccttcg
     1561 cgtagatgag atcgtcgacc gtgtcatcca catagatcaa atgcatcgtg gaaacctttg
     1621 caactccgtc ctcacttact gcgcgacgaa ggacccgccc caaagcttga accctctgcc
     1681 gcaccgcaga cgacgacgct accgaaattc cggtatcagc ttgaggtaca tcgatgccct
     1741 caatgaggga cttcactgag accaacaccg gcgcgtcacc gtcggcgaac gccctgagag
     1801 cttcttcacg gcggcgattc ggcaacttcg aatgctcaag ctcgacccga atatcaggaa
     1861 ggtcagccgc gagggcatcg tgcaattcga ccgcctcttg aacccgttcg tgaaacaaaa
     1921 tcacgcgtgg cctcaaatcc cccttgaaca gcctttcggt aagcaactgt gcaactcgac
     1981 ctcgctcacg ggcacggtaa aggagatctt ttcgctgcgt ggtcagttga acccagatcc
     2041 gcgctgcttc gccaacttcg tcgcttcggc cgacgaggga tctcgccctc cctggttcaa
     2101 atccgcaccc gcgcagtgca tcgccggcat cgtccacccg gcggctaagc gtttcgtatt
     2161 cactccgttc atccgcagca agggagacgc cgtggtggtg aagggtgaac tccggtaacc
     2221 acccggcctc tctcgcatct ttaagcgaaa agcggtaaac cactccccca agggactgcc
     2281 ccacagcctg ctcgtcatac gcgagtggtt cgccgtcctc gtctagttcc tcacggtcgg
     2341 gagttgcgga aagccccaag cgatacttcg ctcgcgtctt cagaacctcc cggtacttgg
     2401 gcgccccggc acggtggcat tcatcgacaa tcagcatcag aggttgtgct gagtcggaca
     2461 tcggcggcag ccttttcgag gccgtgttaa ctacagctac aattgccaac ttatcttgca
     2521 aagtggcttt gccgccagcg ccgagcaaac cgacttcatt ctcatctagt gatgtgtacg
     2581 tcagcagatt ccttttccac tggcgcgcca gtgcctccgt cggaaccacg atcgccaagc
     2641 gaatatctct gtccatttca gcggcccgag ccgcacaaga aatcgcaata agcgtcttac
     2701 cgccaccagt gacaatctcc aatgtgccgg tataaggacg accctcaact ccatggcacc
     2761 atgcgtctac cgccagtgac tgccagggat agagatcaaa gccaatggct gtaaaaccca
     2821 ccacttccct ttcccaagta tcaccccgcc gagtgccgcg accaccaacc ccgctgctcg
     2881 ttcggagctc gccagacgcc ggttactcag tcgcactcgg gtctagacta tcggcacacg
     2941 ggcgaatcac agacgcaatc gatcatgcgt tcaacctcca gggggctact gcgatggaat
     3001 cagatcaaaa ggatgtggcg acccttccgg caatcgcatt cgagcagcga cccgcatgtc
     3061 cacgaatgtt cgtcacagcg gccagagcat ctgaaattct tgagtgggct gatgcacctc
     3121 gcgcgtcggt cgacctcatg gctctttacc aaagggccct cgacccgaaa cgtgcaccag
     3181 aactgagcca gttcatcaaa tccgacaaaa tgaatattgt tcctggcgcc gtaataatcg
     3241 caactgctcc cggttccctg agcgcatccc cttctctgaa tgcacaatca tcgagtggac
     3301 caacggcctg cacagtgacg atttcaatgg ttactccaac gcgggaggtc gcactcagga
     3361 acgccattga acaactgaac agtcggttga gtgcgtccga actcaacgaa gtcaaagaac
     3421 ttgaggggga cgaggagcaa gagaaggagc aactcgagca attcgaggag gaggcgggcg
     3481 acgatgagga ttcaggtctt ccttcttcct acctcaccat cgtcgccgcc gacctagagg
     3541 aagcagcacg ggactgggat tccctagaac acaagaagcg cgccgcaatc gaagaatatt
     3601 tacggtatgc cgctaaaccc ggcatgatca ttgacggcca acaccgagtt tacgcagcaa
     3661 agaatgtaaa cgaatttgac gtaatcttgc ccattgtgct tatcgacgct ctgccgatgg
     3721 ccgagcaggt gttccacttc tacgtactaa acaacaaagc taaaccactc acaaagacag
     3781 atctaaggcg cacgatctcc acagcgttaa ctgcaaccga aatagataca ctctggagtc
     3841 gactcaagga ggcaggagtt gatcccgaca aagtcagact tacgcatctc atcaataccg
     3901 acgataattc tccgtttaag ggtctagtgg acttcggact ggccggagat accggattca
     3961 taaaagaaaa tgtcgcatat caattggttg ataactttgt atcaatgcca gcgcgataca
     4021 gggcactggg agaaggcgta ccggcctggg acagaaaagg agacaaaata gatcgactcc
     4081 ccactttcta cgcattttgg cacgccatca tgaattctta cccgaacgct tggagcgacg
     4141 gagttgaagc acgtggtaag tgtcagttct tcatgaaggt gtccatgctg acactgcaaa
     4201 gcttcgtcct cgacaacctt ctaacaatca acaacgtact ccgaagacag gaccaaccac
     4261 caccctttga ggatctcaac gcactcggcg atcacgtcgt ctcgacaatc gaagatcttc
     4321 ccgaggaatt ctttatgcgg gagtggacag aaaaacagat ggacacttcg caaggcagga
     4381 ggatattacg ggcagcgatc caagaagtta tcgacgcccg aggcaagaac ttgggcaata
     4441 ggtcactctt caaaacgaaa gcttagcacc tagtcttact gcgaatatat ggtggatt
//
LOCUS       DAOPTR010000178         4494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_15629_length_4494_cov_3.701960, whole genome shotgun sequence.
ACCESSION   DAOPTR010000178 DAOPTR010000000
VERSION     DAOPTR010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15629_length_4494_cov_3.701960"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PKI05_04700"
     CDS             complement(<1..741)
                     /locus_tag="PKI05_04700"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOA51531.1"
                     /translation="MTRKYVNQLRPEETVNEVFQVTSRQLRPNRNGILYLQMRLADRT
                     GSLDARMWNVDETILNRFDAGDYVRVEGTTQIFQGAIQLIVSRLQRVPAAEVDPSDFS
                     LVNTLEIDRAMNQLAERLRGINNPHLRALIDSFLLDEEFMAKLAESPAAVSFHHAYRG
                     GLAIHIANMLEMAASIAPLYPLLDKDLLLAGVFLHDIGKINEFAGGSELTYTDSGQLL
                     GHITIGLELLAEAVDKVEKLLGETFPEEL"
     gene            complement(801..3200)
                     /locus_tag="PKI05_04705"
     CDS             complement(801..3200)
                     /locus_tag="PKI05_04705"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOA51532.1"
                     /translation="MRVATATVMLTTWFLWTSAPLRGTTASEPLTHQPPVLRIQLAIS
                     PSVYAFDLRDVRVTAGPFRHAMEMDKRYLLSLDPDRLLHVFRSNAGLPSNVQPLGGWE
                     DPKCELRGHFVGHYLSACSLMFASTGDQSLRERVAYMVAELAKCQERLGGGYLSAFPE
                     EFFDRVETTGRVWAPYYTLHKILAGLLDAYVYCENDQALEVAKKLGDWVIARNQRLSD
                     QQMQRMLGVEHGGMNEALANLYALTGEAKYLQIAERFNHLAVMEPLSRGTDVLTGLHA
                     NTQIPKFIGAARLFEVTGKQWYHDAAVNFWKFVVNERSYVIGGHSDNEHFSPKERLSE
                     ALGPNTTETCNTYNMLKLTRHLFCWKPLAQYADYYERALYNHILASQDPATGMMCYYV
                     PLRSGSHKVYSTPLDSFWCCTGTGVENHAKYGDSIYFHDGKGALFVNLFIPSELQWSQ
                     TGLKLRQETNFPYEPRSTLVITNPPSSDVTIYIRRPFWTTDGFQISVNGEVVTESPTQ
                     EGFVPLRRTWKAGDRIVVDLPMTLRTEAFRDNPHRLAFLFGPVVLAAELPKMSQQQVR
                     KLPPMGSYNPEIPVIVSETIDLGRFKPVEGKPCVWQSPDGLFRSLAEDQAVPVRLEPF
                     FAIHEGRRYVVYWDRVSHQAWKEMIANYQEELVKWRELEKKTIDHVIIGNPESEKQHN
                     LQGENTSHGTFQGRAWRHATNGGWFAYDLQLADKPRLALLVEYWGSDSGNRVFDILVN
                     GQTIATQRLANNRPGHFYRETYPLPDNVLQNGKQVTVRFQAHPGATAGGIFDLRLITL
                     Q"
     gene            complement(3386..4354)
                     /locus_tag="PKI05_04710"
     CDS             complement(3386..4354)
                     /locus_tag="PKI05_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOA51533.1"
                     /translation="MRGFSLSWIVAVAVVICPSWAWAGNQEVAQEIASALKNSGQLRG
                     YKIGVRYQDGIAWLRGSVTDEQQKLAAEKVVSRVPGVKQVVNELAVVPAGATDSPNLQ
                     TAAGAIAPERTIASSSAPSATAQPAAGEVAQRPVQLADPVPSVFAPEPVRPTAAISAM
                     EGPTLAIPELPQADMPMATQTSAMVAPEAAPRPIRPLPIAMTQEEVSREAAPAPAAQP
                     LGRPLPMSVGAPGAAAPARFDQPNMPNYAWPSYAAYPNYAAVTYPKQYSATAWPYIGP
                     FYPYPQVPLGWRKVTLEWHDGWWYLDFDDGASKGPFSGLFRAIKGH"
BASE COUNT         1020 a         1237 c         1241 g          996 t
ORIGIN      
        1 aagttcctct ggaaaggtct ctcccaagag cttttccacc ttgtccacgg cctcggcgag
       61 cagttccaga ccaatggtaa tatggcccaa aagttgtccg gaatctgtat aggtcagttc
      121 cgagcctccc gcgaattcgt ttatcttccc gatgtcgtgg agaaataccc cggccaacag
      181 caagtccttg tcgagcaacg ggtagagagg tgcgatactc gcagccattt ccagcatatt
      241 ggcgatatgg atcgctagac caccgcggta agcatggtga aagcttacgg cggccgggct
      301 ttcagccagc ttggccatga actcctcgtc gagcaaaaac gaatcgatca gggcgcgcaa
      361 atgaggattg ttaattcctc gcagccgttc tgccaattga ttcattgctc gatcgatttc
      421 caacgtgtta accaaggaga agtcagacgg atcgacttcc gccgcgggca cacgttgcag
      481 gcgcgatacg atcaactgga tcgcaccttg aaagatttgg gtggtcccct caacccggac
      541 gtagtccccc gcatcgaacc ggttcaaaat ggtttcatcg acattccaca tgcgggcatc
      601 gagggacccc gttcggtccg ccagtcgcat ctgtaagtag aggattccgt tccggttcgg
      661 acgcaattga cggcttgtga cttgaaacac ctcgttgacc gtttcctccg gccgcaactg
      721 attaacatac ttccttgtca tggatgacct ttcactaaag tgatgatgat agcctattct
      781 cgtagcaaca gtgactgccc tcactgcaga gtgataagcc gcaaatcaaa aattccgcct
      841 gcggtagccc caggatgagc ctggaaacga actgtcactt gcttgccgtt ctggaggaca
      901 ttgtcaggaa gaggataagt ctccctgtaa aagtggcccg ggcgattgtt ggccaatcgc
      961 tgcgttgcaa tagtttgccc attaaccagg atatcaaaga cgcgattacc agagtcactt
     1021 ccccaatatt cgacgagtaa ggccagtcgt ggcttatccg cgagctgcaa atcgtaagca
     1081 aaccacccac cgttggttgc atgacgccag gcccgtcctt gaaacgtccc gtggctggtg
     1141 ttttcgccct ggagattatg ctgcttctcg ctctcaggat tgccaataat gacatggtcg
     1201 atagtctttt tttccaattc acgccacttg accagttcct cttgataatt ggcaatcatt
     1261 tccttccaag cctggtgtga tacccgatcc caatagacaa cgtaccgtcg tccttcatgg
     1321 atggcaaaaa acggctccag cctaacgggg accgcctgat cttccgcgag ggagcgaaac
     1381 agcccatccg ggctttgcca gacacacggc ttgccttcga ccggtttaaa ccggccgaga
     1441 tcgatcgttt cgctgacgat cacggggatt tcgggattat agctacccat tggggggagt
     1501 tttcttacct gttgctggga cattttcggc aattccgccg ccagcacgac tggcccaaaa
     1561 agaaaggcca aacgatgagg attatcacga aaggcctccg ttcgcaacgt catgggtagg
     1621 tcaacaacga tacgatcgcc agccttccaa gtgcggcgaa gggggacgaa gccctcttgc
     1681 gtagggcttt cggtgacgac ctcgccattt acgctgatct gaaatccgtc cgtcgtccaa
     1741 aaaggtcggc gaatgtaaat agtcacatct gacgatggcg ggttggtgat aaccaaggtg
     1801 gaccgcggtt catagggaaa attggtctct tgccgcagtt tcagccccgt ttgtgaccac
     1861 tgtagttcgg aggggatgaa caggtttaca aatagagcgc ccttgccgtc gtgaaagtag
     1921 atgctatccc cgtactttgc gtgattttcg acacctgttc ccgtgcaaca ccagaaagaa
     1981 tcgagcggag tcgagtaaac cttatgggag ccggaacgaa ggggcacgta ataacacatc
     2041 attcccgtcg ccggatcttg ggaggcgaga atgtgattgt ataatgcccg ttcgtaatag
     2101 tcggcatatt gggcaagggg cttccaacag aaaagatgtc gcgtcagctt cagcatgtta
     2161 taggtgttgc atgtctcggt tgtgttagga ccgagtgctt ctgaaaggcg ttcttttggc
     2221 gaaaagtgct cgttgtcgct gtggccgccg atgacatagg accgttcgtt gacgacaaac
     2281 ttccaaaaat tgacggcggc atcgtgatac cattgttttc cggtcacctc gaacagtcgc
     2341 gcggcgccga tgaacttggg aatttgagtg ttggcatgaa ggccggtcaa aacatccgtg
     2401 cccctggaga gaggctccat cacggctagg tggttgaacc gctcggcaat ctgcagatac
     2461 ttcgcttcgc cggtcaaggc atagaggttg gcgagggcct cgttcatgcc cccgtgttcc
     2521 acgccgagca tccgttgcat ttgctggtct gagagacgtt gattgcgggc gatgacccaa
     2581 tcacctaact tcttggccac ttccagggct tggtcgtttt cgcagtaaac ataggcatcg
     2641 aggagaccag ccaggatctt gtgaagtgtg taatacggtg cccagacccg cccggtggtt
     2701 tctacacggt caaaaaattc ctcaggaaag gcactgaggt aacccccgcc gagccgctct
     2761 tggcatttcg ctaattccgc caccatgtag gcgactcgtt cccggaggga ttgatcgccc
     2821 gtgcttgcaa acatcaaaga acatgccgac agatagtgcc ccacaaaatg tccgcggagc
     2881 tcgcacttag gatcctccca gccgccgagg ggttggacat tactgggtaa tcccgcgttg
     2941 gagcgaaaga catggagcaa acgatcggga tcgaggctca gcagatatcg cttgtccatt
     3001 tccatcgcat gtcgaaaagg acctgccgta acacgtacgt cgcgaaggtc aaacgcatac
     3061 acgctggggg aaatggcaag ctggattcgc aagacaggtg gttgatgcgt gagcggttcc
     3121 gatgccgtcg tgccgcgcag cggcgcactc gtccacaaaa accaggttgt cagcataacg
     3181 gtggcggttg cgactctcat acagttgccc tttctaaaac cagcctcaac cgagcgtagg
     3241 tgtattacat ggacaatcca ttactatacc ctatcggacc cgcagcggcc cggtcaatga
     3301 tcgccaccgc caagaaacag agaaacccca ccggtcaagg gtggggtttc acgaacatca
     3361 atcaggcggg aatgatgtct gctgctcagt ggcctttaat ggctcggaag agtcctgaga
     3421 acggtccctt cgatgcgcca tcgtcgaaat caagatacca ccagccgtcg tgccactcca
     3481 aggtcacctt ccgccatccc aacggaactt ggggataggg atagaagggg ccaatatagg
     3541 gccaagctgt tgccgagtac tgcttgggat aagtgacggc tgcgtagtta ggataggcag
     3601 catagctggg ccaagcgtag ttaggcatat tcggttgatc gaaccgagcc ggggcagcgg
     3661 ctccaggagc gccaaccgac attgggagag gacggcccaa aggttgggct gcaggtgccg
     3721 gtgcggcttc gcgggagacc tcttcctgtg tcatagcgat gggcaggggc cggatcggac
     3781 gcggagcggc ttctggagct accatcgcgg atgtttgcgt tgccatgggc atatccgcct
     3841 gcggcaactc ggggatagcg agtgtcgggc cctccatagc ggatatggcc gctgtaggac
     3901 gaaccggctc aggggcaaaa accgacggta caggatcggc tagctgaaca ggtcgttggg
     3961 caacctcacc cgccgccggt tgtgcggtcg ccgagggagc actggaggat gctattgtac
     4021 gttccggggc aatggctcct gcggcagttt gcagattcgg cgaatcagtg gcaccggcgg
     4081 gaactaccgc gagctcgttg acgacttgtt tgaccccagg gacccgggag accacctttt
     4141 cggcggccag cttctgttgc tcatcggtca cactaccccg aagccacgca atcccatcct
     4201 ggtaccggac tccaatcttg tagccgcgca attggccact gtttttcaat gcgctggcga
     4261 tctcttgtgc cacttcctgg ttgcctgccc aggcccatga cgggcaaatg acaacggcaa
     4321 ccgccacaat ccaactcaat gaaaaccctc gcatgcctgt cctcctgttc ggaatccacg
     4381 tcccgacggt cggcgccttg ttctggaagg cagacttcct ttcccgcctg gatcagttac
     4441 ctgagaaacc taacctcatc ttcaccggac gtcatagaca aaacgttgct taca
//
LOCUS       DAOPTR010000179         4492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_15637_length_4492_cov_4.783412, whole genome shotgun sequence.
ACCESSION   DAOPTR010000179 DAOPTR010000000
VERSION     DAOPTR010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15637_length_4492_cov_4.783412"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PKI05_04715"
     CDS             <1..214
                     /locus_tag="PKI05_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51534.1"
                     /translation="AFAARLRAAASGIDPDALADRAAALREGREAEERQVAEDAARER
                     ELERQQELEKERDSIAQRDRGLGHGL"
     gene            775..1278
                     /locus_tag="PKI05_04720"
     CDS             775..1278
                     /locus_tag="PKI05_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51535.1"
                     /translation="MTFKVSGFIKKLSMSRRPNPAEGPSHRDFVARPILRADHSREFS
                     WKNENVLILGQETGLHDLAKGISALGASVSFRFLMQLQELYQLPLEQYSTIIMTDRSD
                     GQQCDVIDVGGILRRADSNLRLVWASKQFKLSAVATTADGRFCDVQLALPASPSHLEL
                     FLRSPLR"
     gene            complement(1264..1605)
                     /locus_tag="PKI05_04725"
     CDS             complement(1264..1605)
                     /locus_tag="PKI05_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51536.1"
                     /translation="MKHVENSAVWINVIASLMAAGVDRRLAVGIVDILVENALQSHDE
                     HHTPLILSKCQMLDAPSKEAKVMEGVDQIMPRLFSITQQLLAAPSLKRKHEAQQYCRH
                     AVLIEGGSAQG"
     gene            2065..2502
                     /locus_tag="PKI05_04730"
     CDS             2065..2502
                     /locus_tag="PKI05_04730"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOA51537.1"
                     /translation="MSYTLGEAAKAVGFSKPTLSRAIKNGKLSAKRLDDGSYSIDPAE
                     LERWKDANGHRNGAVKRIATHKETPETPRETSALQDEVEKLRAQVQSDQRERGFLEQR
                     IEDLKERAERAERKEDQLHALLVDQRPQATVEPRKGFWGRLFG"
     gene            complement(2506..3513)
                     /locus_tag="PKI05_04735"
     CDS             complement(2506..3513)
                     /locus_tag="PKI05_04735"
                     /inference="COORDINATES: protein motif:HMM:NF021637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HOA51538.1"
                     /translation="MSLLPDRHPNLDFFVLDIADAVPKDDMASMEHPLFSLATKPDMR
                     HLEYRSGDNVLKIRPSGLGLPTIFDKDILIFTISQLMHRKNRGEAIGDTVRFSARELS
                     VATNRQIGGDHYKRLENAFARLQGTQFVTNIRTGGKIETRIFSLIDEGGFVRTDDERF
                     RLDYCEVKLSRWLMRAIESDQVVTISNDYFRLRRPLERRLYEIARKHCGNKPKWQIGL
                     VTLQNKTGSNAPAKRFRHNLREIIKADVTPFYRFELDDADLVIVRPRQPKAQITPAIA
                     LPDWAEEKARAVAREKGRDYYALRADWLAFAQAETAKGNPPKNAGAAFVAYCAKQDSL
                     R"
     gene            3855..4109
                     /locus_tag="PKI05_04740"
     CDS             3855..4109
                     /locus_tag="PKI05_04740"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOA51539.1"
                     /translation="MGMTTINSRTFNQDASGAKRAAQDGPVFITDRGKPAHVLLSIEA
                     YRRLTGRQESILDLLADPEAADVAFEPDRLDRLTRPADLS"
     gene            4109..>4492
                     /locus_tag="PKI05_04745"
     CDS             4109..>4492
                     /locus_tag="PKI05_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOA51540.1"
                     /translation="MFILDTNVVSELRKAKAGKADANVVAWAEDQDPSALFLSAITLL
                     ELEMGVRQMERRDSTQGAVLRAWMADRVLPVFDERILPVDGPVALCCAALHIPDPRSD
                     RDALIAATGITHAMSIVTRNVADFEP"
BASE COUNT          980 a         1197 c         1321 g          994 t
ORIGIN      
        1 ggcgtttgcg gcgcggttgc gcgcggcggc gtcggggatc gacccggacg ccctcgccga
       61 tcgtgccgcc gcgttgcgcg aggggcgcga ggccgaggaa cggcaggttg cagaggacgc
      121 ggcacgggaa cgcgagctgg aacgtcagca agagcttgaa aaggagcggg acagcatcgc
      181 ccaacgcgac cgcgggttgg gccacgggct atagaaggga gaggtggtct agatgggtgg
      241 tgtaacttta cgtctggaaa tccatttggc gctggcacca tgagtccctg ccgaggccac
      301 cgccataaac tgtccggtca cacatcatct cacgcttaat ttttaagcaa ggtagtgaca
      361 atcaacgctc attggccctg tgcgcacctc tccccagact tgtccactct ttctgtggac
      421 aactttctga cgtaggcttc actggaaaat gaaaagaaaa accttcacca taaaggtgaa
      481 ggcaaggagt gggcgtgatc tcctgaaagg gagatttacc atccttttca cggtgccggg
      541 gttccgccaa ggctgttaat caccattaca ggtcaatgtg gcgccaaaga cactgctgag
      601 ggaggatgga gcgtaacgtc cacgcatgtc ccagccgcgc ccctaacgat ctgaccgcaa
      661 ccggaagcct gagttcttca tcagagacaa cattgcacgc aataggggtg ctgagtaagc
      721 tgctattcgc acctgtccag atcaaggcag gctttcaggt agaagttgga agtaatgacc
      781 ttcaaggttt ctggatttat aaaaaaactg tcgatgagca ggaggcctaa tcctgctgaa
      841 ggaccctctc acagagattt tgtggctcgc cctattttac gggctgacca ttcccgggaa
      901 ttttcttgga agaacgagaa cgttcttatc ctggggcagg aaaccggact tcacgatctg
      961 gcaaaaggta tttctgcgct cggcgcatcc gtgtcattcc gcttcttgat gcaattacaa
     1021 gaactctacc aactcccgct ggagcaatat tccacaatca tcatgaccga taggtctgat
     1081 ggccagcagt gcgatgttat cgacgttggc ggtattctga gacgtgcaga ttccaacctc
     1141 aggcttgtgt gggcatcaaa gcagttcaaa ttgtctgctg ttgccaccac agcggatggt
     1201 cgattttgcg atgtacagtt ggcgcttcct gcatctccgt ctcatttgga actcttcctg
     1261 agatcacccc tgcgctgacc cgccttctat aagaaccgcg tgcctgcaat attgctgggc
     1321 ctcatgtttg cgcttcagac ttggggccgc gagaagttgc tgcgtgatcg agaataagcg
     1381 gggcatgatt tgatcgacac cttccatcac ctttgcctcc ttgctgggcg catcaagcat
     1441 ttggcatttt gaaaggatga ggggcgtatg atgttcatca tggctttgga gagcgttctc
     1501 taccagaata tcgacaatcc cgacagcgag tcttctgtca actcccgctg ccatgaggct
     1561 tgcgataaca ttgatccaaa cggcggagtt ctctacatgc ttcatggcaa ccggctttcc
     1621 ttgttcgagg cttagaaagc caatataaag ttggcgtaaa caaggcacct ataaggtatt
     1681 tgatagacaa gacagggaga ggtgaagtgg tccttggaca tataatcctg ggcatggtgc
     1741 tcggcataat ttctgccgcg atcagtctct ttctcggatc gtctctcgta tgggcacttg
     1801 tcatgtacgt tgccatggga ggtcttggca ccctcctatc agcggccatc gcctatgctt
     1861 tgccaagact ccgaaagcgg gagccctccg aggaatgcaa acggctgtag agcgcctctc
     1921 cgatcctgcc tccgatatct ggtgtctccg gcaggatcgg agaggcgttt ggagatcgcg
     1981 ccaacagagt gtaacgtgtg taactgtaag cctcccaggc agatgaggcg aattgcaacc
     2041 ctgtgagagc gagcggttac acccatgagt tacaccctag gagaagctgc gaaagcggtt
     2101 ggtttcagca agccgacgct ttcacgggcc ataaaaaacg ggaagcttag cgcgaagcgt
     2161 ctggatgacg gttcctacag catcgacccg gcagagctgg aacggtggaa ggatgctaac
     2221 gggcacagga atggtgctgt gaagcgaatt gcaacccata aagaaacacc tgaaacaccc
     2281 cgtgaaacca gtgctttgca ggacgaagtt gagaagctcc gcgcccaggt ccaatccgac
     2341 cagcgcgaac gcggttttct ggaacaacgc atcgaggatc tgaaagagcg ggccgaacgg
     2401 gcggaacgca aggaagacca gctccacgcg ctcctggtcg atcagcggcc gcaggcgacc
     2461 gtagagccac gcaaggggtt ctggggccgg ttgtttggat agccgctagc gcaggctgtc
     2521 ctgtttcgca caataggcga caaaggccgc ccctgcgttc ttcggcgggt tgcccttggc
     2581 cgtctccgcc tgcgcgaagg ctagccaatc ggcccgcagc gcgtagtagt cccggccctt
     2641 ttcccgcgcc acggcgcggg ctttctcttc cgcccaatcg ggcagggcaa tggcgggggt
     2701 gatctgcgcc ttgggctggc ggggccggac gatcacgagg tcagcatcgt ccagctcgaa
     2761 gcggtagaac ggcgtcacgt ccgccttgat gatttcgcgc agattgtgcc ggaaccgttt
     2821 ggcgggggcg ttgctgcccg tcttgttctg gagcgtcacc agtccgatct gccacttcgg
     2881 cttgttgccg caatgcttcc gcgcaatttc atagaggcgg cgttccagcg ggcggcgcag
     2941 ccggaagtag tcgttcgaga ttgtgacgac ttgatcggat tcgatagccc gcattagcca
     3001 gcgcgacagc ttcacctcgc aatagtctag gcggaaacgt tcgtcgtctg tgcggacgaa
     3061 cccgccctcg tcgatcagtg agaaaattcg cgtctcgatc ttcccacctg tccgaatgtt
     3121 ggtgacgaac tgtgtcccct gcaagcgggc aaaggcgttc tcaagacgtt tatagtggtc
     3181 gcccccgatc tgacggttag tcgccacgga cagctcgcgg gccgagaagc gcacagtgtc
     3241 cccaatggct tcgccccggt ttttgcggtg catgagctgg ctgatcgtga aaatcaggat
     3301 gtccttgtcg aaaatggtgg gcaggccgag gccggacggc cggattttaa gaacgttgtc
     3361 cccgctacga tattcgaggt gccgcatgtc cggtttcgtt gccaatgaga acaacggatg
     3421 ctccatcgaa gccatgtcgt cttttggcac cgcatctgca atgtcgagga cgaagaaatc
     3481 gaggttcggg tggcggtcgg gcagcaagct catcagcgat tctttggcct atcaggaact
     3541 ttcgcgtttg gcctatcagg aaccgatggg gctaagcaag ccgtttggcc ctttcggaac
     3601 caaagtttgg ccctttcgga accaaagttt ggccctttcg gaacccggag gcaactttat
     3661 cttattggga gcaagaggtt gttgaatccg taacttactc cgtaggtaac actattaaca
     3721 ctagggcaaa cgctgatcga atgggtgggt agcagagagg ggaagctagt catgaaggat
     3781 tcaggttgac attacagcat aatgtaggta cgtttctaca ttaaggttgc ttagaagtta
     3841 gaaggatgtg agcgatgggg atgacaacga tcaacagtcg gacgttcaat caagacgcca
     3901 gtggcgcaaa gcgggctgcg caagacggac ctgtgttcat cacagaccgg ggcaaacccg
     3961 ctcatgtctt gctcagcatt gaagcctaca ggcggcttac aggacgccaa gaaagtattc
     4021 ttgatttgct tgccgatcca gaagcggcag acgtagcttt tgaaccggat cgcctggatc
     4081 gcctcacacg tcccgctgat ttgtcctgat gttcattctc gatacgaacg tggtgtccga
     4141 gctgcgtaag gcaaaagcag ggaaggcgga tgccaatgtc gttgcgtggg ccgaggatca
     4201 agacccgtca gcgctgtttt tgtccgccat caccctcttg gaacttgaga tgggcgtcag
     4261 gcagatggaa cggcgtgaca gcacgcaggg cgccgtcttg agggcttgga tggctgatcg
     4321 ggtgcttccg gtgttcgatg agcgtatctt gcccgtggac ggacccgtgg cgctttgctg
     4381 tgccgctttg catattccag acccccgcag tgaccgggac gccctgatcg ccgcgacagg
     4441 gatcacccac gccatgtcca tcgtcacacg caatgttgcg gacttcgagc cg
//
LOCUS       DAOPTR010000180         4489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_15649_length_4489_cov_3.120207, whole genome shotgun sequence.
ACCESSION   DAOPTR010000180 DAOPTR010000000
VERSION     DAOPTR010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15649_length_4489_cov_3.120207"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PKI05_04750"
     CDS             complement(<1..539)
                     /locus_tag="PKI05_04750"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOA51541.1"
                     /translation="MEKAGVAVAFLYDVRQLVPQVVFQYDLLVILRDGMLWPDPQPNG
                     SFGKPVFWLTEEQEMTVASFVWAGGGFLALHNATALKALNETESLYLRVLGASYAGHG
                     PAQEGYYVRVLNGSHPVTAGVHDYFVEDERHWPRLYVSDALVLLESASGEETSLHGFV
                     RTFGLGRVCYLANGHNRRV"
     gene            complement(593..979)
                     /locus_tag="PKI05_04755"
     CDS             complement(593..979)
                     /locus_tag="PKI05_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51542.1"
                     /translation="MGSRFSTRKSRQSGLCWGTALRVAAIALVLVGWVLADPTGTAVA
                     EALNPPAFSTIDVLPEGERTWLRDAVAYQISMGRSLDMAVREIKALKSFPEQWSEPQL
                     AALVGAMYQRLTAPPQGRRGVPGPWP"
     gene            1529..2182
                     /locus_tag="PKI05_04760"
     CDS             1529..2182
                     /locus_tag="PKI05_04760"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51543.1"
                     /translation="MPFLMQWKGKLPMGMVYHEMVMAFDIHATALAAAGIPLPTDKPL
                     DGVNLLPFLTGKQSGRPHEQLFWRAGRKYAARVADWKLVQESVGGPQLFNLKDDLGEQ
                     HDLAQKYPDKLKELQAAFAEWEKGTQPAKWVRQDSRNAEIGGKLKAGGASAPARPGGG
                     LESRFKQYDKNGDGKLTPAEYPLSFLAEADKNNDGALTLDEVKAYYVGRRQKRTSEK"
     gene            2921..>4489
                     /locus_tag="PKI05_04765"
     CDS             2921..>4489
                     /locus_tag="PKI05_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51544.1"
                     /translation="MAVDSREGIAAASADGFVYLFSLDGKLRWKHDLGAPVLQVAFAK
                     LDGKSLVVLATGVSRELIALSTSGERLAAAKLNGAGRMMRAGDFDADGADEVVVLPIR
                     GQAMNAFFFKGPNLAKLVESIPSGTRMWDPEMGQRKKVGNAEPSAWNTLSLKKANGTV
                     ADLNGDGAMELIYSPGAYTLKNGLRQLVALPGPFKTASYDQYYNMRLVAAGNLTEEPG
                     AEIVVVEGPQVRLYDSTGKELGKAIAPFGFTDVVYSPGVPYGSVLLGSSPNGDDNIYR
                     LTFKPGWEKTLEALERRGVMAGIGTTLKQIADAVAGWQGEPMPGTDGPYDVVVNHSLW
                     SGWDPKKFDAWIAEVRAYEEAFPYSRLRFSYCIWPGEDAPLIRPDGKPWGRDHRLAHD
                     LTREQIVAAAKYFEAKGCYFWVQVGHGCDPHLEVATVAAMLEAAPKTLLGFVSAEDEQ
                     LDKVPYYFEHHVKPILELCLKHKKRFIPRNKDVWWAHWPADAKMRELIFNPRYRSVIV
                     PSVEDSNSRSPEVNL"
BASE COUNT         1006 a         1275 c         1312 g          896 t
ORIGIN      
        1 aagacacgtc ggttgtgtcc atttgcgagg tagcataccc tacccaaacc aaacgtgcga
       61 acaaaaccat gtaaggaagt ctcttcaccc gaggcggact ccagcaaaac aagggcgtcc
      121 gaaacataca agcgcggcca gtggcgctcg tcctccacga aatagtcgtg gacccccgcc
      181 gtcacgggat gcgagccgtt caagacacga acgtaatagc cctcttgggc agggccgtgg
      241 ccggcataac tcgcaccgag gacgcgaaga tagagcgatt ctgtttcgtt taaggctttc
      301 aaggccgttg cgttgtgcag cgcaaggaat ccaccgccgg cccacacgaa tgaggccacg
      361 gtcatctctt gctcttccgt gagccagaag accggtttgc cgaaagaacc gttcggttgc
      421 ggatcgggcc aaagcatccc atcccgaagg atgaccagca aatcgtactg aaacacaacc
      481 tgcggcacaa gttgccgaac gtcgtacaga aacgcaaccg ccacccccgc cttttccatg
      541 gcctcctcca gcggtggtcg aatataggcc ggatcgtgat accggtcgcc gatcagggcc
      601 agggcccggg gacgccgcga cggccttgag gcggagccgt cagcctttga tacatcgccc
      661 ccacgagagc ggccaattga ggctcagacc attgttcggg gaaagacttc agagccttta
      721 tttcacggac tgccatatca agtgagcgac ccatggatat ttggtatgct accgcatctc
      781 gcagccaggt tcgctctccc tcgggcaata catcgatggt cgagaaagcc ggtggattta
      841 acgcctcggc aacggcggtg cccgttggat ccgccaaaac ccaccccaca agcaccaacg
      901 cgatcgccgc aacgcggagc gccgtgcccc aacacagtcc tgactgccta ctctttcgag
      961 ttgagaatcg ggaacccatg atatgtctcc tggtgcgaag ctcgagaaac gcgcagcgga
     1021 actgcaacac cgttttttag cgacttggga tcagaaatct tcacgactcc ttttgcgact
     1081 gatcgatcac acgaccacgt gaagtcatcg tatggaatca acagacaggt cgccgaaaag
     1141 aattgcgacc ggtcccgatg ggcgggcatt cgcaatccca aacctagggc gaaacacggg
     1201 ccgccgtgct cgaaaagaag ccccaaaaac cccatctatc tttcttggag tgtaattccg
     1261 gaaaggtcgg cttacaaatt tcgcagtcgg aatcttccat tccgtgcccg gaaaaccggg
     1321 gccttcgcct caatgcatgt gtcccggcgg ttggggtgaa acactagcga tcggctggcc
     1381 gattgcgctg ggcaggtgca gtggtgtggg aagcaactta ggggcgccga ttccgcgagg
     1441 tagctctcga cgatctcgga gtacgtcccg ccgagcatgc ggtgcttctc aatccccttc
     1501 cgggcctctg gcaggagggg gcatccgcgt gccgttcctt atgcagtgga agggtaaact
     1561 cccgatgggc atggtctatc acgaaatggt catggctttc gacattcacg ccacggcatt
     1621 ggcggctgcc ggtattccat tgccgaccga caaaccactg gacggcgtga accttctccc
     1681 tttccttact ggaaagcagt cgggccgccc acacgagcaa ctcttttggc gggccgggcg
     1741 gaaatatgct gctcgcgtgg ccgactggaa gctcgtgcaa gagtctgtcg gcgggccaca
     1801 actcttcaat cttaaagacg accttggcga gcagcacgac cttgcgcaga agtatcccga
     1861 caaacttaag gaactgcaag ccgctttcgc cgaatgggaa aaaggcacac aaccggccaa
     1921 atgggtccgg caagattccc gcaacgcaga aattggcggc aaattgaagg cgggtggtgc
     1981 ctctgcccct gctcgtcctg gcggcggtct cgaaagtcgg ttcaagcaat acgacaaaaa
     2041 cggcgacggc aaactgacgc cagccgagta tcctctatct tttcttgcgg aagctgacaa
     2101 gaataacgac ggtgccttga cgctggacga agtgaaagcc tactacgtcg gccgccgaca
     2161 aaaacgaacg agcgagaaat gagaaactaa tcttcgaaca gtttcggcgc ccctttccgt
     2221 tgtcatttcg gcgctccgtc gaggtcctgg ggaggacacc ggcggagccg cgtggctaca
     2281 tgattcacca agacgaaccg gtcggagcga ggcttcacac ccggccttgc ggatgatgcg
     2341 ggcaaatcgg gtccgcatgg tggtccggat cccgctttca ggatgactgg cggtttggcg
     2401 atagacgccg agggaaacga cgtattccgc ccctggctcc gcttcctcct ctcgagtcag
     2461 agacgtcggc ggaaaccgaa aaatcgtacc aattaagacg tgtccccgga gtgagaacga
     2521 ccgatggagc aggacccggg tacgctacaa cgacctcacc tctcgtttcc tagtttgccc
     2581 cggtgacttg acgcgttcca gggtgccgag taatagtgct tgctggttcc acaccggcgc
     2641 aactcggtgc tgaaatatgt acgtgacagc ctggcaaata aaggccaaat gagagtattg
     2701 tgccatgcct ttcagcagac aatttttgtg ttgcctattg attggtgcaa acgccgcgtt
     2761 cgccgacgtc gagattattc caaccggagg gcggaatatc taccgactgg caactcaacc
     2821 ggggctgatt gtcggctcga cgtacgacaa tcgcgtttgc gcgttcaccg ttaccggaaa
     2881 gcacttgtgg gatgtgccca caggcggatt tgtgttcgac ttggccgttg acagtcgcga
     2941 agggattgca gcagccagtg ccgatggttt cgtttactta tttagcttgg atggtaagct
     3001 ccgctggaaa cacgatctgg gcgcgccggt cctgcaagta gcctttgcaa agctcgacgg
     3061 caaatcactg gtcgtgcttg ccaccggcgt gtcgcgcgag ctgatcgcgc tctctaccag
     3121 cggtgagcgc ttggcggctg cgaagctaaa cggcgcgggt cgcatgatgc gggcgggcga
     3181 tttcgacgcc gacggggcgg acgaggtggt cgtattgccc attcgtggcc aagcaatgaa
     3241 tgcatttttc ttcaaagggc caaacctcgc caaactcgtg gaatccatcc cgagcggcac
     3301 gcgaatgtgg gacccggaaa tgggccaaag aaagaaagtg ggtaatgcgg aaccatcggc
     3361 gtggaatacc ttgtcgttga aaaaggcgaa cggcaccgtc gccgacctca acggcgatgg
     3421 cgcgatggaa ctgatttatt caccgggcgc ctacacactt aagaatggcc tgcgacagtt
     3481 ggtagccttg ccggggccgt tcaagacagc cagttacgat cagtattaca acatgcgtct
     3541 tgtggccgct ggcaacctca ctgaggagcc tggcgcggaa attgtggtcg tcgaaggccc
     3601 ccaggtgcgt ctctacgatt ccaccggcaa ggagcttgga aaagcgatcg caccgttcgg
     3661 gttcaccgat gttgtctatt cgccgggtgt gccgtacggc agtgtcctgc tcggctctag
     3721 cccgaatggc gacgataata tctaccgact cacctttaag ccaggctggg aaaaaacgtt
     3781 ggaggcactt gagcgacgcg gtgtcatggc cggcatcggc acgacactta agcaaattgc
     3841 cgatgcggtt gccggatggc agggcgagcc catgccgggg accgatgggc cgtacgatgt
     3901 tgtcgtgaat cactctctct ggagtggctg ggaccccaag aagttcgatg catggattgc
     3961 tgaggtccgc gcctatgaag aggcttttcc gtattcgcgt ttgcggttct cctactgtat
     4021 ctggcccggc gaagatgcac cgttaatacg cccggacggg aagccatggg gacgtgacca
     4081 ccggttggcc cacgacctga cgcgtgagca aatcgttgcc gctgcaaaat actttgaggc
     4141 aaaaggctgc tatttctggg tacaggtcgg ccacggctgc gatccacacc tggaagtggc
     4201 cacggtcgcg gcgatgttgg aggccgcccc gaaaacgctg ctggggttcg tcagtgccga
     4261 agatgagcag ctggataaag taccgtacta ttttgagcat cacgtcaagc cgattttgga
     4321 attgtgcctg aaacacaaga aacgcttcat cccgcgcaac aaagacgtct ggtgggcgca
     4381 ctggccggca gacgccaaga tgcgtgagtt gattttcaac cctcggtatc gctctgtgat
     4441 tgtgccgtca gttgaagaca gcaattcgcg cagtcccgaa gtgaacctc
//
LOCUS       DAOPTR010000181         4487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_15656_length_4487_cov_3.222924, whole genome shotgun sequence.
ACCESSION   DAOPTR010000181 DAOPTR010000000
VERSION     DAOPTR010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15656_length_4487_cov_3.222924"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>4487
                     /locus_tag="PKI05_04770"
     CDS             <1..>4487
                     /locus_tag="PKI05_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HOA51545.1"
                     /translation="GKSLCYFLPIVDSLVRAPDTGDRVAALVVYPMNALVNSQFQALN
                     RLKERYEARMGRPFPVTFAKYTGDTAEDVRAQMRRHPPQIMLTNYVMAELLLVRPEDQ
                     RFLERAMPSPPTPLPEGEGRCPGTLLPEGEGRARGGLRFLVFDELHTYRGRQGADVAM
                     LIRRLKERCAARDLVHIGTSATMVASRQATPAERRAAVADFAGRLFGHSFQAEDVIEE
                     TLVPFTQGGPPTVEELRKAMTDGDWQKDAESFRRHPLVRWVEYALGIEKDAEGNLRRR
                     VPRTLSGAADELAELTGIARHRCQEYLRQVLELGGAMPGEGGNRALAFKLHQFISQGR
                     ALFATLEPAPQREFSLEGQTQAGGGRIFVPVKFCRQCGQDYYHVLQTNPPSPSGRGVG
                     GEGTSPSPFGRGVGGEGCFLPHPIGIELGQDEEEQQAGYLMLAPAEDDWSEDRIPEEW
                     YDASGRFKNSWRDRVPTAVWVAPDGTYWRNSRAGAIKMWWQKEPFSLCLRCGEFYTAK
                     ERDFAKLASLSSEGRSSATTVLAVSLLRHASGGDGPRDKLLSFTDNRQDASLQAGHFN
                     DFVHVSLIRCALYAALKQKSELTFSEVAKHVVECSGLTIRDIARNPELAPESAAARDV
                     WKVFTELTEYRLYEDLRRGWRVVQPNLEHVGLLRIGYRGLEDLCAGEKYWQFHPNVAR
                     LSPAERETIVRAVLDQFRRKLAIRCDCLRETEQQQIRKRAEQQLNEFWGLDPDANELC
                     PAARYVRLGQSRQLALGYRLGERSAIGRFLRQRLGLSTDEYLTFLDALLKLLVEQGFL
                     VREVKDDHQFYQLDAACLVWRLGDGTPPPDPMYSRRVPPVNAFFQRFYQEWPGFLAGL
                     EAREHTAQVVRPGERERRERRFRWEESDTQKEREVGRRLPYLVCSPTMELGVDIADLD
                     FVHMRNVPPTPANYAQRSGRAGRQGQPGLIFTYCGALNSHDQYFFNRRDEMVAGSVRP
                     PRLDLTNEALLRAHVHAMWLAQVRLPLGQSIEDVIDREQDDLPLKTEVAGAIQLGEAA
                     RYELRRRVQNVLAPDMGILEQTGWFNEDWIDRVLDEAPQQFDRAFDRWRELYRAAVQQ
                     REAAYNELKRARTIEDQDKADRKMKEAQRQLNLLCQIDVAREEGDFYPYRYLASEGFL
                     PGYNFPALPVRAWVPRDEGEFIGRPRFLAIRELAPNNILYHEGTKWEVVSLQSPPGGL
                     DERLVRRRLCHICGAFCESTFDLCPVCQTRFDGQNSLLATMLDMPNVRLRRRERITCD
                     EEERRRLGYDIQTAFQFAGSPDKPRVQRADVFVGTTPVLHLIYAPAATLLRVNHGFRM
                     AKQPGFWVDFESGEVHTKPPQEKNAPPRRSRIQNIRLAVQGTQNMLLVQLVRPELRDD
                     SILETSLQYALQRGCEQLFQLEESELSTERIGKGKHRAILFYEAAEGGAGVLRRLVEE
                     ADAISRLAREALAICHFDEQGNDLLPKCQAACYQCLMSYGNQHEAMQLDRHRILQTLR
                     DL"
BASE COUNT          903 a         1273 c         1446 g          865 t
ORIGIN      
        1 gggaagagcc tttgctattt tctgcccatt gtggacagcc tggtccgcgc accggatacc
       61 ggcgaccgcg tggccgcgtt ggtggtctat cccatgaatg ccttggtgaa ctcgcagttt
      121 caggccctca accggctcaa ggagcggtac gaggcccgca tgggacggcc attcccggtc
      181 acgttcgcaa agtacaccgg cgataccgca gaggatgtcc gcgcccaaat gcggcgccat
      241 ccaccccaga tcatgctgac caactacgtg atggccgaat tgctgctggt gcggcccgag
      301 gatcagcgct ttttggagcg ggcaatgccc tcacccccaa cccctctccc agagggagag
      361 gggaggtgtc caggaacgct tctcccagag ggagagggga gagcgcgtgg cgggctgcgg
      421 tttttggtgt tcgatgaatt gcacacttat cgcggccgcc agggggccga cgtggcgatg
      481 ctcatccgca ggctgaaaga acgctgtgcg gcccgtgacc ttgtccatat agggaccagt
      541 gccacgatgg tggccagccg ccaggccacg cctgccgagc gtcgtgccgc ggtggcggac
      601 tttgcggggc ggttgtttgg ccattccttc caggccgagg acgtcatcga ggaaacgctg
      661 gtgccgttca cccaaggcgg cccgccgacc gtggaggagc tccgaaaggc aatgaccgac
      721 ggggattggc aaaaggatgc cgagagtttt cgcagacatc cgctggtgcg ctgggtggaa
      781 tacgccctgg ggatcgaaaa ggacgccgag ggcaatctta ggcggcgtgt tccaaggacg
      841 ctttcagggg cagcggatga acttgcggaa ttgacgggca tcgcgcggca ccgatgccaa
      901 gaatacttgc gacaggtcct ggaactgggc ggggccatgc cgggagaagg cggcaatcgg
      961 gccctggcct ttaagttgca ccaattcatc agccaaggcc gggcactgtt tgccacgttg
     1021 gaaccggcac cacaacgtga attctcgttg gagggtcaaa ctcaagctgg gggtggccga
     1081 atcttcgtcc ccgttaagtt ctgccgccaa tgcggccagg actattatca cgtgctgcaa
     1141 accaatcccc cctctccctc tgggagaggg gtagggggtg aggggacttc cccctcgccc
     1201 ttcgggagag gggtaggggg tgagggctgc ttcctgccgc atcccatcgg catcgagctt
     1261 ggccaggacg aggaggaaca gcaagcgggc tacttgatgc tggcgcctgc cgaggatgat
     1321 tggagcgagg accgcattcc cgaggaatgg tacgatgcca gcggtcgatt taagaattcc
     1381 tggcgcgacc gagtgcccac cgcggtctgg gtcgccccgg acggcacgta ctggagaaat
     1441 tctcgggctg gggcgatcaa aatgtggtgg caaaaggaac cgttctcgct gtgtctgcgt
     1501 tgtggcgaat tctacacggc caaggagcga gacttcgcca agttggcgtc gctctccagc
     1561 gaaggacgct cgagcgcaac caccgtgctg gctgtatctc ttttgcgtca cgccagcggt
     1621 ggcgacgggc cgcgcgataa gctgctgagc ttcaccgaca atcgccagga cgcttccctt
     1681 caggccgggc atttcaacga tttcgtccac gtctcgctga ttcgttgtgc gttgtatgca
     1741 gccctgaagc aaaagtcgga gctgaccttc tctgaggtag ccaaacacgt ggtcgagtgc
     1801 agcggtctga cgatccgaga cattgcccga aatcccgaac tggccccaga atcggccgca
     1861 gcccgggatg tgtggaaggt cttcactgag ctgacggaat accgtctcta cgaggatctc
     1921 cgtcgggggt ggcgtgtggt ccaaccgaac ctggagcatg tcgggctttt acggattggg
     1981 tatcgcgggc tagaggacct gtgcgctggt gagaaatact ggcagtttca ccccaacgtc
     2041 gcgcggttgt caccggcaga gcgggaaacc attgtccggg ccgtgctgga tcagttccgc
     2101 cgcaaactgg cgatccggtg tgattgtctt cgggaaaccg agcagcagca gatccgcaaa
     2161 cgggccgaac aacaactcaa cgagttctgg gggcttgacc cggacgccaa cgaactttgc
     2221 cccgccgcgc gatacgtgcg actaggacag tcgaggcagt tggcgctggg atacaggctg
     2281 ggcgaacgca gtgcaattgg caggttcctc cgacaacggc ttggcctttc cacggacgag
     2341 tatctgacgt ttctggatgc cctgctgaaa ctgctggtgg aacaaggttt tctggttcgt
     2401 gaagtaaagg acgaccacca gttctatcag ctcgacgcgg cctgtttggt gtggcgactg
     2461 ggagatggaa cgccgccgcc cgacccgatg tattcacgtc gtgtgccgcc ggtcaacgct
     2521 tttttccagc gcttctatca ggagtggccg ggctttttag cgggacttga ggcccgtgaa
     2581 cacacggccc aggtggtcag gccgggcgag cgcgagcgcc gcgaacgccg tttccggtgg
     2641 gaagagagcg atacgcagaa ggaaagagag gtgggacgcc gcctgcccta tttggtgtgc
     2701 tcgccgacga tggagcttgg cgtcgatatt gcagatcttg acttcgtcca catgcgaaat
     2761 gttccgccga cccctgccaa ttatgcccaa cggagtgggc gggcaggacg tcagggtcaa
     2821 ccagggctga tatttaccta ttgcggtgcc ctgaacagtc acgaccagta cttctttaac
     2881 cgccgggatg agatggttgc tggtagcgtc cgaccgccac gcctggattt gaccaatgaa
     2941 gccctcctgc gggcccacgt gcacgccatg tggctcgccc aagttcgcct gcctttgggg
     3001 cagtccattg aggacgtgat cgacagggaa caggacgatt tgcccctcaa aacggaggta
     3061 gccggggcga ttcagctcgg tgaggcagcg agatacgaac tgcgccgtcg ggtgcagaac
     3121 gtcttggcac cggatatggg aattctggaa caaacgggct ggtttaacga ggattggatt
     3181 gaccgcgtcc tggacgaagc accgcagcaa ttcgatcggg cgttcgatcg gtggcgagaa
     3241 ctttaccggg cggccgtgca gcagcgagag gcggcctata acgagcttaa gcgggcgcgc
     3301 acaatagaag accaggataa agccgatcgc aagatgaagg aagcccagcg gcaactcaac
     3361 ctgttgtgtc aaattgatgt cgcgcgagag gaaggcgact tttatccgta ccgatatttg
     3421 gcgagcgagg ggttcctgcc gggctacaac ttcccggcct tgccggtgcg ggcatgggtg
     3481 cctagggatg aaggcgaatt tattggccgt ccgcgttttc ttgccatccg cgagttggcg
     3541 ccgaataaca ttctctatca tgaggggacc aagtgggagg tcgtcagttt gcagtccccg
     3601 ccgggagggc tcgatgagcg tctggtccgg cgccggctat gccacatctg tggtgccttt
     3661 tgcgagagta cgtttgacct gtgtcccgtt tgccaaaccc ggtttgacgg ccagaatagc
     3721 ctgctggcga ccatgctgga catgcccaat gtgaggttga ggcggcgcga acgcatcacc
     3781 tgcgacgagg aagagcggcg tcggctcggc tacgacatcc aaactgcatt ccagtttgcg
     3841 ggtagcccag acaaaccccg ggtgcaaaga gcggacgtct tcgtcggcac caccccggtc
     3901 ctccacctga tttacgcacc agcggccacg ttgctccgcg tgaaccacgg ctttcggatg
     3961 gccaagcagc caggtttctg ggtggacttc gaaagcggcg aggtacacac gaaacccccg
     4021 caggaaaaga atgcccctcc gcgacgtagc cggatccaga atatccggct cgctgtgcag
     4081 ggaacgcaaa acatgctgct cgtgcaactc gtgcgacccg aattgcgcga cgattcgatc
     4141 ttggaaacat ccctgcaata tgccctgcaa cggggttgcg agcagctctt ccagcttgag
     4201 gaatcggaac tgtccactga acggatcgga aagggaaagc accgggcgat tctcttttat
     4261 gaggcagcgg aaggaggcgc cggcgtcctc cgccggttgg ttgaggaagc agatgccatc
     4321 tcgcgcctag ctcgggaggc gctggctatt tgccacttcg acgaacaagg taacgacctt
     4381 ctccccaaat gccaggcagc atgttaccag tgcttgatga gctacggcaa ccagcatgag
     4441 gcgatgcagt tggaccgcca tcgcatactt caaaccctca gggacct
//
LOCUS       DAOPTR010000182         4467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_15750_length_4467_cov_3.037171, whole genome shotgun sequence.
ACCESSION   DAOPTR010000182 DAOPTR010000000
VERSION     DAOPTR010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15750_length_4467_cov_3.037171"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="PKI05_04775"
     CDS             complement(<1..915)
                     /locus_tag="PKI05_04775"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOA51546.1"
                     /translation="MKLCSRAGTTITLQQLLPDLQVFGDGELQVGVCHSQPSRIKPGD
                     LFVLLHPPGPRRDAAIEQAVGRGCAAILSEDVLPGLSVPVICVSDVRTAYGRICHALH
                     GFPTQKLGVIGVTGMYGKTTTSCLVARILVQAGYSVGMLTSLGYFEGEESTFPVETTP
                     PPDELAARLARMADNECTHAVVEVSAKAIKQRYTAGLEFDTVVFLNDEAEGASSHDAR
                     CRWFSLVDNLRPEGLAIFNADDSRCRAALAETDHPALTVGIHAAAEIKAREIHRHLGE
                     ETFYICVGDDTFPVRTKMFGRHHVRNCLA"
     gene            1268..1831
                     /locus_tag="PKI05_04780"
     CDS             1268..1831
                     /locus_tag="PKI05_04780"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOA51547.1"
                     /translation="MFEVFEHTADLGIRVRAPSLEGLFKEAAEAMIQLMCRGPQLEDT
                     TSAPTGATPSAQAASPPTEALRATPTELREVTIPAEDLPIPEPQPSPCETSSDNGSRI
                     VLSPTWEDLLHDWLSTILYLFSVERLIPVDYQIQFDSMGLKARLKVRAYDPVRDGGEV
                     EIKAVTYHGLRIEKEANEFTAQVIFDV"
     gene            complement(1849..2517)
                     /locus_tag="PKI05_04785"
     CDS             complement(1849..2517)
                     /locus_tag="PKI05_04785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA51548.1"
                     /translation="MNRRLSAILPFRNAEHVVLNLIPSYVDVFSELSSDWEILAVDDG
                     SEDSTPEILCEFAQMYPQVRVAIHGQARGFYASLCSGLSLSVGNILFLPLRYQPMTLD
                     SLHRLWSAVQNHSLVIGLAANEFGQDEIECLMLQRRVASPILPALSSTERLAAVVNRF
                     ESSAVIVPLRRVAFSQEKPYASYGRTFTTTNGREAMPRAPKSLTLQAAKAVFSRIKQF
                     ALDE"
     gene            complement(2945..4231)
                     /locus_tag="PKI05_04790"
     CDS             complement(2945..4231)
                     /locus_tag="PKI05_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOA51549.1"
                     /translation="MPDWTFPSIFRLRQKFPSHRIDDIPARIVAEFERANVRNRVRPG
                     QTVAITAGSRGIANIVLILRTVVEIRHQWGARPFIVPAMGSHGGGMVEGQRGVLHTYG
                     ITEDSVGCPIRATMDTVVVGRAPEGFDVHFDRFAYEADHVVVVNRIKPHTLFVGPIES
                     GLMKMLLIGLGKHRGAEIYHRAAWDYSFDRIVRDVGRIVLSRCRILLGLAIVEDAYDA
                     TAHLEAVLPEAFETREPELLRLAKEWMPRLPFDHAHVLLIDQLGKDLSGSGIDTNIIG
                     RKFNDHRAAPGEKPDIRFICVRGLSPGTHGNAIGLGLAEFCRSDILPQIDVHATRINA
                     ITSGHVSAAMVPLDYPTDREMLGQALANIGMTPPEHARLMWIADTLHLTEVECSAAYL
                     AEAKARDDLEILTGVRPMPFGDDGNLPDMMTLRASS"
     gene            complement(4296..>4467)
                     /locus_tag="PKI05_04795"
     CDS             complement(4296..>4467)
                     /locus_tag="PKI05_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOA51550.1"
                     /translation="GLNKQNYWWYIDLRRFGTVPHSGFGLGLERALQFITGMGNIRDV
                     IPFPRTPGSAEF"
BASE COUNT         1050 a         1299 c         1211 g          907 t
ORIGIN      
        1 ggcaaggcag ttccgaacat ggtggcgccc gaacatcttt gtgcggaccg gaaacgtatc
       61 gtcccccaca cagatgtaaa aggtttcctc gccaagatgt cggtgaattt cccgagcctt
      121 gatttcggcg gccgcgtgaa tacccacagt caatgcggga tggtccgttt cggccagggc
      181 cgcccgacaa cgtgaatcgt ccgcgttaaa aatggccagc ccttccggtc gtaggttatc
      241 gaccaacgaa aaccaccgac atcgggcatc gtgtgaagag gcaccctctg cctcatcatt
      301 aagaaacacc acggtgtcaa attccagacc ggcagtgtat cgctgcttga tggccttggc
      361 agaaacctcc acaacggcgt gcgtgcattc gttgtcggcc atccgggcaa gccttgcggc
      421 taactcgtct gggggcggcg tcgtctccac aggaaaggtc gattcttccc cttcgaaata
      481 gcccaggctc gtgagcattc ccacagaata accggcttgc accaggatgc gggccacaag
      541 gcatgaggtt gtcgtttttc cgtacatccc cgtcacgccg atcacaccca gcttttgggt
      601 aggaaagccg tgaagggcgt ggcagatccg tccgtaagcc gtgcgaacgt cggacacgca
      661 aatcaccggg acagataaac ccggcaggac gtcttccgag aggatggcgg cacatccacg
      721 ccccaccgcc tgctcaatag cggcgtcccg gcgaggtccg ggcgggtgca gaagaacaaa
      781 cagatcaccc ggcttgatcc ggctcggctg gctatggcac acccctacct gcaactctcc
      841 gtcgccaaac acctgaaggt ccggcaggag ctgctggagc gtgatcgttg tgcccgctct
      901 ggagcataac ttcatcggcc gaggcctccg tacctcggat gattacggac aaacctacaa
      961 tgctcgccca tcaagtggga aaacgacgtt acaattgatg accacttccg tgtggtcaag
     1021 tctgacgggg attgtgctga ttttccggct tttgtcaagg cgaattccgg agaggggcct
     1081 tctgctgaga tgcagagcca cgctgtcgtg gccggatcga acggcccgtg caccgctgtg
     1141 tactccaatg acttttaacc ctcggattgg caaggcaccc cgtgcaacca attctccgaa
     1201 gacggttcgg ggtataggat atgtcaagcg acgtccctca aattgtcggg aaaaggagga
     1261 gagcttgatg tttgaagttt ttgagcacac cgctgatctc ggcatcaggg tacgagcacc
     1321 gagccttgaa ggactcttca aggaagcggc agaggccatg attcagctca tgtgccgggg
     1381 gccacagttg gaagacacga ccagcgcccc aactggagcg actccttccg ctcaggctgc
     1441 gtcaccgcca accgaagcgt tacgggctac accgacggaa ttgagggagg tgaccattcc
     1501 ggccgaggat cttcctatcc cagaacccca gccaagtcct tgcgaaacgt catccgacaa
     1561 tggctcacgg atcgtactgt cccccacttg ggaagacctt ctccacgatt ggctgtcaac
     1621 aattctctat ctattctcag tggaacgcct tattcccgtg gattatcaga tacagtttga
     1681 ctcaatgggt cttaaagcac gactgaaggt ccgcgcgtac gatccggtcc gagatggtgg
     1741 ggaagtcgaa atcaaggccg tgacctatca cgggctgaga atcgagaagg aagccaatga
     1801 gtttacggct caggtcattt ttgacgtgtg aggaggcgga tccccaagtc actcatccag
     1861 ggcaaactgc ttgatgcggc tgaaaacggc cttagcagcc tgaagggtca aggatttggg
     1921 agcacgtggc atcgcttccc gcccgttggt tgtggtaaag gttcgtccat aactggcgta
     1981 aggcttctcc tgcgaaaatg ccacccgtcg cagcggcacg atcaccgcag aagattcaaa
     2041 ccggttgacc accgcagcca gtctttctgt ggacgacaac gcgggcagaa tcggcgacgc
     2101 cacccgccgc tggagcatga gacattcgat ctcatcttgg ccgaattcat tggcagccaa
     2161 tccgatcaca agggagtggt tctgcactgc cgaccagaga cggtggagcg agtcgagcgt
     2221 catcggttgg taccgcagcg gcagaaacaa gatattccct acactaagcg acagaccaga
     2281 acacaaagag gcatagaatc cgcgggcctg gccatgaatt gctacccgaa cttggggata
     2341 catttgggca aattcacaca agatctcggg cgtgctatcc tccgagccat catccaccgc
     2401 caggatttcc cagtcactgg acaactccga gaacacatcc acgtaactgg gaatcagatt
     2461 caagaccaca tgctcggcat tacggaacgg caggatcgcg gacagcctac ggttcaagat
     2521 cgtgctcctt gtggctggaa gaccaccaca aactgatggg cggatggtca atcgagacgt
     2581 ctcgcttcct tgctgaaaag ccatgcggaa gccagctcgg cccctatcgg ctcataaagt
     2641 ccacctttct tcttttcgaa gaaatccgcc gttccctcaa ttctctcgca cgaataacgt
     2701 tttcttccgg ataagccaaa gatacgacaa gataagggca attcttttgt atcgaatgcg
     2761 tcttcccgtg attgtttcaa ctgctcagag ccatctcaag aagccccctc tccctctggg
     2821 agagggttgg ggtgagggca agcgaacgtc gcaatcgatt aagaaaaccg gcagcgattc
     2881 gagccaccct cacccccgac ccctctcccg ccaaacgggc gaggggagac tatgggatag
     2941 gctgttagct ggaagctcgc agggtcatca tatccggcaa gttcccatca tcgccaaatg
     3001 gcataggccg aacccccgtc aagatttcca ggtcatctcg ggctttagcc tcggccaggt
     3061 aggccgcaga gcattcgact tcggtgagat gcagcgtgtc ggcaatccac atcaatcggg
     3121 cgtgttctgg tggcgtcatg ccgatattcg ccagggcctg ccccaacatt tcccggtctg
     3181 tcgggtaatc gagcgggacc atagccgcgg aaacatgacc agatgtgatg gcgttaatcc
     3241 tcgtagcatg gacgtcaatt tgtgggagta tgtcactccg gcaaaactcc gcaagaccca
     3301 aaccgattgc gtttccatgt gtccccggac tcaacccgcg aacacagatg aagcggatat
     3361 cgggcttttc acccggagcc gcacgatggt cgttgaactt tcggccgata atgttggtat
     3421 cgattccgga accacttagg tcttttccca attgatcaat aagaagaacg tgggcatggt
     3481 caaatggtag ccgtggcatc cactctttgg cgagcctcaa aagctctggt tcgcgggtct
     3541 caaaagcttc gggcaggaca gcctccaaat gggccgtggc atcgtaggca tcttccacga
     3601 tggccagtcc caaaagaatc cggcatcgcg aaagaacaat ccgtccgacg tcccgtacga
     3661 tgcgatcaaa ggaataatcc cacgcagcac ggtggtaaat ttcggccccg cggtgttttc
     3721 ccaacccaat gagaagcatt ttcatcaggc cactctcaat cggaccgaca aaaagggtat
     3781 ggggcttgat gcgattgacg accaccacat gatcggcctc ataagcaaaa cggtcgaaat
     3841 ggacgtcaaa tccctccggg gcccgtccta cgacgacggt gtccatcgta gcacgaatgg
     3901 gacaccccac cgaatcctcg gtaatcccgt aggtatgcag gacacccctt tgcccttcca
     3961 ccataccacc cccgtgacta cccatggcag ggacgatgaa aggtcgggcc ccccactggt
     4021 gacgaatctc cacgactgtg cgaaggataa ggactatatt ggcgatgccg cggctgcccg
     4081 ccgtaatggc cactgtctgc cccggacgga ccctgttccg aacgttggca cgttcaaact
     4141 cggccacgat tcgggccgga atatcgtcga tgcgatgaga gggaaacttt tggcggagac
     4201 gaaagatgga tggaaacgtc caatcaggca tgaatgatca ctcttgcgaa tcggtaactc
     4261 cccggcgagg aagccgtttt acgccctttt tcctgctaaa attccgcact gcccggcgtc
     4321 cgggggaacg gaatcacgtc gcgaatattg cccatccccg tgatgaactg gagtgccctt
     4381 tccaagccta ggccgaaccc gctatggggc acggtaccaa atcgacgcaa gtcgatgtac
     4441 caccaatagt tttgcttatt gaggccc
//
LOCUS       DAOPTR010000183         4449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_15827_length_4449_cov_4.098316, whole genome shotgun sequence.
ACCESSION   DAOPTR010000183 DAOPTR010000000
VERSION     DAOPTR010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15827_length_4449_cov_4.098316"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PKI05_04800"
     CDS             complement(<1..634)
                     /locus_tag="PKI05_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51551.1"
                     /translation="MPAVHPGPTSVFVHSHEASGKLVVEFARNPKDFPLNNYVQIQTS
                     QKPAGYYLVMTSEQAARVINADLREFMWAPGNEAPDGNWNVESFEFEPFECKRYAYPF
                     RMDMETARNASWDIVAQHARYAAQQAMTARTVKVLSELTNSTQWPASHVLSVPTIPGN
                     DSTKWDGATVANQVIRNSITYAAELILNDTLGVVRPSDLRLVISSGLARVM"
     gene            complement(656..1069)
                     /locus_tag="PKI05_04805"
     CDS             complement(656..1069)
                     /locus_tag="PKI05_04805"
                     /inference="COORDINATES: protein motif:HMM:NF021473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HOA51552.1"
                     /translation="MSGSPLIAGGDILPCRFVRVTGDHQVQQGGDNTPVIGVAGPSAK
                     YPPLSNLVTSNYHARTGDPVFFFGDGEECLIEAGAAFSAGTRLKSDSNGRAVAIATSG
                     TTIQHIGAIAKEAATAAGQLVRCIVKIYSERPALS"
     gene            complement(1066..2532)
                     /locus_tag="PKI05_04810"
     CDS             complement(1066..2532)
                     /locus_tag="PKI05_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51553.1"
                     /translation="MATVSLDQQLDSEQFVHIPRVPIFREHYLRLPDGRVIYFDRAKL
                     QQIADNCNRRIEQTGDYATIVINHTGRSEDTPCVGFAGPFYVEPLPLEEETVWAITAD
                     FHIYRDYKDVLKHYPKRSVELYNDGPFEKWFLDPIALISKTPRLDLGLLYSRDRSGGI
                     RIRYSAAEPGPTSVYVPEMGKRQRKTDYAAPAAAAAGAQTALAAVEKIPEMVQKGAEG
                     IVDMAARAVYELPSESIDPQKAKQILHDKEVRGHPLTEKQRKMFGAAASRAEYEQQEA
                     KSMALSKEDLDAIVQAFLETPVGQWIQKKMAAEEEGTSSGPENGATTPVVDAGEAKAK
                     TEYEKEAAENAAEASIEALKEQIELLKKQLEEERSKRTDVERYSKLKQLRARFIFDLD
                     KEFARCRRMNDQQFDLHVKEVIPNYKENPASGLIPAEMTDTSGYPTESLKYERHPDGS
                     ISYTVYGSEAVAKAVQLATTKGLTFEEALQQVVGGVKS"
     gene            complement(2767..3051)
                     /locus_tag="PKI05_04815"
     CDS             complement(2767..3051)
                     /locus_tag="PKI05_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51554.1"
                     /translation="MSEPQNRPRPRTRAEALELAKNFRKIPTTLTEPKPPPKELPPEE
                     YQKAEAEYEQKMKQYWLESLYGANYEENPIYQWREPYWDNEWEAIKDLIL"
     gene            complement(3048..>4449)
                     /locus_tag="PKI05_04820"
     CDS             complement(3048..>4449)
                     /locus_tag="PKI05_04820"
                     /inference="COORDINATES: protein motif:HMM:NF017850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HOA51555.1"
                     /translation="FEIWGYPAGSKEAEERVRQAATQRIGEHRNVVLVPQWPDSAGFG
                     GFNYQRIEPSMAGAEAARVIVEYFSHVIKRYILGQTLTSEAANTGLGSNLGEIHLATF
                     QQIVRYDARNLEESITTDLVRPLLEHNFPEFAEEDFRFVIKTEDENVSERLELLERAY
                     RMGLAIKAEEIRKLVGISPPGPEDEVVQLPGQGQESQTTVPFNYARAKGIQYSLPEGL
                     PENPEEGTRVTIKGKTYAYIPNAAGKLQWQVVDETKSEPSQLKEPSASPKKEEEHAGP
                     PQKASKLPQVKFGPTTPQRFIQFRDSLPADKRAYLSQYTAEEIEDKIKAGAKIFQTED
                     GTAGYMLVPIPDRPGEYDLCNVFRTPQGPKGGGQAAVIEAISQGATTLDCIGDGLAKI
                     YHKFGFKVYKVVPWDDQYAPEGWDYEKNDRPPIYFMRYIGDTRDPEEIRQRAETGYYG
                     DFDADQYPEGYEEPWP"
BASE COUNT          847 a         1393 c         1200 g         1009 t
ORIGIN      
        1 ccatgacccg ggccagcccg gaggaaatga ccagccgcaa atcggacggc ctgaccacgc
       61 ccagggtgtc gttgagaatc aattccgcgg cataggtgat gctgttgcgg atgacctggt
      121 tggccaccgt ggcaccgtcc cattttgtac tgtcattgcc tgggatcgtg ggcacggaaa
      181 gcacgtgcga ggcgggccat tgggtggaat tggtcagctc gctgagaact ttgacggtcc
      241 gcgcggtcat cgcctgctgg gccgcgtaac gggcgtgctg ggccacaata tcccagctcg
      301 cgttgcgggc ggtctccata tccatgcgga aaggataggc gtaccgcttg cactcgaacg
      361 gctcgaactc gaaactttcg acattccagt tgccgtccgg ggcttcattg ccgggggccc
      421 acatgaattc gcggaggtcc gcattgatga cccgggccgc ctgctccgaa gtcatcacca
      481 gataataacc ggcaggcttc tgggaggttt gaatctggac gtaattgttg agcggaaaat
      541 ccttcggatt ccgcgcaaat tccaccacca gtttgccgct cgcctcatgc gaatggacga
      601 agaccgacgt gggaccagga tgaacagcag gcatattctt gcacctccat cagcctcagc
      661 tcaaagcagg ccgttcgctg taaatcttca caatgcaacg gaccaactga ccggccgccg
      721 tggccgcctc cttggcaatc gcaccgatgt gttggatggt cgtaccactt gtggcaatcg
      781 ccacggcacg cccgttgctg tcactcttga gacgggtccc ggcagaaaag gcggccccgg
      841 cctcgatcag acactcctcc ccgtcgccaa agaagaacac cggatcaccc gtcctggcgt
      901 ggtaattgga tgtcaccaga ttgctcaaag gcggatactt ggcgctggga ccggccacgc
      961 caatcacggg cgtgttgtcg cccccctgct gaacctgatg atcacccgtc acccgcacaa
     1021 agcgacaggg cagaatatcg ccaccggcaa tcaaggggct tccactcatg acttcactcc
     1081 tccaaccacc tgttgcaagg cttcctcgaa tgtcaggcct ttcgtcgtgg ccagctgcac
     1141 ggccttggcc accgcttcgc tcccatagac ggtgtaactg atgctcccgt caggatgacg
     1201 ctcgtacttg agcgattccg tcggatatcc cgacgtgtcg gtcatctccg ccgggatcag
     1261 accgctggcc gggttctcct tgtagttcgg aatcacctcc ttgacgtgga gatcgaactg
     1321 ctgatcgttc atccggcgac agcgggcgaa ttccttgtcc agatcgaaga tgaaccgggc
     1381 ccgcaactgc ttgagcttgc tgtaccgctc gacatcggtc cgcttggagc gctcctcctc
     1441 aagctgcttc ttgaggagtt caatctgctc cttgagggcc tcgatgctgg cctcggcggc
     1501 gttttccgcg gcttccttct cgtactcggt cttggccttg gcctccccgg catcgaccac
     1561 cggtgtcgtc gcaccgtttt ccggaccgga ggacgtccct tcctcttccg ccgccatctt
     1621 cttctgaatc cactgaccga ccggcgtctc caaaaaagcc tgcacaatcg cgtccaagtc
     1681 ttctttgctc aaggccatag acttcgcctc ctgttgctcg tattccgcac ggctggccgc
     1741 cgcaccgaac atcttccgct gcttctccgt caaaggatga ccgcgaacct ccttgtcgtg
     1801 gagaatctgc ttcgccttct gggggtcgat gctctcgctg gggagttcat agacggcccg
     1861 cgccgccata tcgacgatcc cctcggcccc cttctggacc atctcgggaa tcttttccac
     1921 cgccgctaag gccgtctggg caccggcggc cgccgctgcc ggggccgcat agtccgtctt
     1981 ccgctgtcgc tttcccatct ctggcacgta cacactcgtc ggccccggct cggcggcgct
     2041 gtaacgaatc cgaatcccgc cgctcctgtc cctggaatac aagagtccca gatcaagccg
     2101 gggcgtcttc gaaatcaggg ctatcggatc aagaaaccac ttctcaaacg ggccgtcgtt
     2161 gtacagctcc acactccgct tgggatagtg cttgagaacg tccttgtagt cccgatagat
     2221 gtgaaaatcc gccgtgatcg cccaaacggt ctcctcctcc aagggcaggg gttccacata
     2281 aaagggaccg gcaaagccca cacagggcgt gtcctcgctc cttcccgtgt ggttgatgac
     2341 aatcgtggca taatcccccg tttgctcgat ccgccggttg cagttgtcgg caatctgctg
     2401 gagcttcgcg cggtcaaagt aaatgacccg cccgtccggc agccgcaggt agtgctcgcg
     2461 aaagatcggc acccgcggga tatggacgaa ttgttccgaa tcgagttgtt gatcaaggct
     2521 tactgtggcc atctcttgtg aatactcgtt ggggcgttac tcgactaata ccagaaaacc
     2581 gaagattgaa gtcaataacc cgatcagtgg ggattagacc atataagaag gtgactattg
     2641 gcgggataga taggaataga ggaccaccgg gctaataccg ggcaaaaaag aaccgcctgt
     2701 gcacggcgtc acaggcggcc ttcgtggagg gcgccacgag aggattcaaa acagacaaga
     2761 caaagtttac agaatcaggt ctttaatggc ttcccattcg ttgtcccagt agggttctcg
     2821 ccactggtaa atggggtttt cttcataatt cgccccgtat aggctttcta accagtactg
     2881 tttcattttc tgttcgtatt ccgcctcggc cttctggtat tcctccggcg ggagttcctt
     2941 cggcggtggc ttgggctccg ttagtgtagt cggaattttg cggaaattct tggccaactc
     3001 gagcgcttcg gcacgagtcc gcggtctagg acgattttgc ggctcactca tggccatggc
     3061 tcctcgtagc cctccggata ctgatcagca tcgaagtcgc cataatatcc agtttctgcc
     3121 cgctgccgaa tctcttctgg gtctcgggtg tccccgatat atcgcatgaa ataaatcggc
     3181 ggtcggtcgt tcttttcgta atcccacccc tcgggggcat actgatcatc ccagggcaca
     3241 accttataca ccttaaaccc gaacttgtgg tagattttcg caagaccatc gccgatgcaa
     3301 tcgagtgtcg tggccccctg actgatggcc tcaatcaccg ccgcctgtcc cccgccctta
     3361 ggtccttgcg gggtgcggaa cacgttgcag aggtcatact cgccaggcct atcggggatc
     3421 ggaaccaaca tgtaaccggc tgtcccgtcc tctgtctgga agattttcgc acccgcctta
     3481 attttatctt caatctcttc cgcagtgtat tgcgagagat aggcccgctt atcagctggt
     3541 aaggaatcac ggaactggat aaaccgctga ggcgtcgtcg ggccaaactt gacctgagga
     3601 agtttgctgg ccttctgcgg aggaccagcg tgttcttcct cctttttcgg ggaagccgac
     3661 ggttctttca gttgcgatgg ttcgcttttc gtctcgtcaa cgacttgcca ctgaagcttg
     3721 ccagccgcat tgggtatgta agcataggtc ttgcctttga tcgtgacccg tgttccttcc
     3781 tctgggttct ccggcaaacc ttcagggaga gagtactgga tgcctttcgc ccgcgcataa
     3841 ttaaagggaa ccgtcgtttg ggattcttga ccctgtcccg gcagctgcac gacctcatct
     3901 tccgggccgg gcgggctgat cccgaccagt ttcctgatct cctcggcctt aatagccagc
     3961 cccatccggt aggcccgctc caaaagctcc agacgctcgc tgacgttctc atcctccgtc
     4021 ttgatcacaa accggaagtc ttcctcggca aactccggaa aattgtgctc caaaaggggc
     4081 ctgacgaggt cggtcgtgat cgactcttcc aggttgcggg cgtcatagcg gacgatctgc
     4141 tggaaggtcg ccaggtgaat ctccccgaga ttgctcccca gacccgtgtt ggccgcctcg
     4201 ctcgtcaggg tctgtcccag gatgtaacgc ttgatgacgt gcgagaaata ctcgacaata
     4261 acccgcgccg cctccgcccc cgccatcgag ggttcaatcc gctggtagtt gaaccccccg
     4321 aagcccgccg aatccggcca ctgggggacc aggaccacat tccggtgctc gccgatcctt
     4381 tgcgtggccg cctgccggac ccgctcctcc gcctccttcg atcccgccgg atagccccaa
     4441 atctcaaac
//
LOCUS       DAOPTR010000184         4449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_15828_length_4449_cov_3.756941, whole genome shotgun sequence.
ACCESSION   DAOPTR010000184 DAOPTR010000000
VERSION     DAOPTR010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15828_length_4449_cov_3.756941"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            743..1393
                     /locus_tag="PKI05_04825"
     CDS             743..1393
                     /locus_tag="PKI05_04825"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOA51556.1"
                     /translation="MPKIVDLTLPMLDGGPSYPKDPQLRLKDFRKLPEDGCNVTEITM
                     GSHQGTHLDAPSHFLQNGKSLDQIPLERFYGPAVLVDLAPDGELPPKTPITPDMLQPF
                     APLFREGHRVLCRTGWYNHFSQPEYFTDLPSLTAEAARWIAARRISLLGIDMPTPSKI
                     AGRECHEVLLGPGAEIVLVEGLTNLHLLPREFIFVGFPLNLPGRDGSPIRAVAIIE"
     gene            1480..2457
                     /locus_tag="PKI05_04830"
     CDS             1480..2457
                     /locus_tag="PKI05_04830"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HOA51557.1"
                     /translation="MTSPMGKNGSATNLRGHEYEEADFRAAMPAPKEGIFLPGTMIEV
                     IRVPTSPGTYRHVLFDFDGTLSLIREGWPDVMIPMMVECLLETGTQETEEELEDTVRA
                     FVMELTGKQTIYQMIRLAEEIKKRGGRPLDPTEYKRMYHDRLMARIAHRREGLRSGQI
                     RPEEMLVPGSLELLEELQRRGVQLYLASGTDEVYVREEATLLGLDRYFGEHIYGAIDD
                     YKRFSKAMVIEQILATHRITGNLLLGFGDGYVEIQNVKEVGGTAVAVASDEAKKSGQP
                     DPWKRERLISVGADLVIPDYRDWQTLIAYLWGEITAPCVPRESLGKECV"
     gene            2481..>4449
                     /locus_tag="PKI05_04835"
     CDS             2481..>4449
                     /locus_tag="PKI05_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOA51558.1"
                     /translation="MGYPQFDRSRLILRPLAERTHDIDRSILLDPTTVQSSFHHPAIP
                     TLAVRILEARKRGAAVIAFCGAHVLRTGNGPLLIDLMEKGLLTHLALNGAGAIHDFEL
                     ALIGATCESVARYIRTGEFGLWQETGRINDAAIIAAREQIGFGEALGRMIETERFPYR
                     ETSVLAAGYRLRVPVTVHVAIGQDIVHEHPNFDPVAIGQATYTDFLIFTHSVANLEGG
                     VFLNIGSAVAGPEVYLKALSMARNVARQEGREIRDLTTAVFDLVAIGSDWRTEAPKTD
                     PRYYYRPFKTVLVRTVADGGTSFYVQGDHRETVPALYRTLTTFQKELDTLDTTVEWRS
                     QFERRGASRMKQVERFAGFRKDRLEEILSRFKNLRVAVVGDFFLDKYLDVDPRLAETS
                     LETGKLAHQVVRVRHSPGAAGTIVCNLAALGIGTIYCVGLIGDDGEGYELRRDLVNLG
                     CHIEHLHVCPDAMTPTYLKPRDITDPSLAGEHSRYDFKNRSQTPPELRQKIANSLSTL
                     LPQVDAVIVLDQVEEADCGVVTKDLRELIAELAPCHPGVIFWADSRQSIREFRHIHLK
                     CNQFELLGNALDPWRPVPDWDILIPAMEEFRKVNNAPIVVTLGKDGMLVSDPAPTWIP
                     GVRVSEPTDPTGAGDSATAGAVASLAAGATLP"
BASE COUNT         1014 a         1206 c         1252 g          977 t
ORIGIN      
        1 gggtaacgat tccgtgtcaa ccaggctagg agccgagaaa ccggagatcg acgccatgtc
       61 agattcccat cccaaaaaaa cagatcccga cgcctaccgg gccccattaa gcaggccaat
      121 gttaacgaac tatcggcggt tccctaggga aactctagaa cactttttgt gaaagatcaa
      181 aagataggca agctaggaag attggggaca ccccaaccag accggtgcgc ggtatctata
      241 aaacgaagcc gcgtcccgat cattccatgg gttcgggacg cggccgcttc cgggagggac
      301 aaaggggaac ggggaatcca aggacaggga gtgtttcagc tcacccggtt actcccgagc
      361 atgatcgcat taaatcggcc tcctccgaat atttctctct gaactcaagc caccgacctc
      421 gttctttgaa ccatctgcac cacaaccatc atttatgcct aattggcatg tctaaaatat
      481 catatacatt tgcccgcgct gcggtcaagc ccgatatccc cagaaatctt cccgcccatt
      541 caaacgaccg atgccaccaa atcggcggca ggttacggcc agatcgaccc gatccgcaga
      601 agtccctcgc ctcttgccaa gggatcatct catttccgag aatgaccgtt tgcgacagta
      661 cctttccgcc cgagactgat ttgaccttta aggcagacca ctcggcttga tctgctttgc
      721 cctttgttag gagataacga ccatgccgaa gattgtggat ttgacactac ctatgctgga
      781 tggcggtccg agctatccga aggatcctca gcttcgcctc aaagattttc gcaaactccc
      841 ggaggatggg tgtaacgtca cggaaatcac gatgggctcg caccagggga cacatcttga
      901 cgccccatct catttcctac aaaacggaaa gtcgttggat cagattcctc tggagcgatt
      961 ctacggtccg gctgttctcg tggacctggc ccccgacggt gaacttcctc ccaagacgcc
     1021 gatcaccccc gacatgcttc aaccttttgc cccgctgttt cgtgaggggc atcgggtact
     1081 ttgccggacg gggtggtaca accactttag tcagccggaa tattttaccg atctaccatc
     1141 tctgaccgcc gaggccgccc gctggatcgc cgcccgacgg atcagcctgc tcgggattga
     1201 catgccgacg cccagtaaaa tcgccggtcg agaatgccat gaagtgttgt taggaccggg
     1261 cgcggagatc gtccttgtgg aaggtttgac aaacctccat cttctgccac gagaattcat
     1321 ctttgtgggt tttccgctca accttcccgg ccgagacggg tcccctatcc gagcggtggc
     1381 catcatcgag tagttcactt tcgtgtactg cacacgacat agtgcaatag tgcgagaatc
     1441 acccggtgtg gcttgaacca gagaattacc gaaacagata tgacatcccc tatgggcaaa
     1501 aatggtagcg ccacgaattt gcggggacac gagtacgagg aagccgattt tcgcgcggcc
     1561 atgccagccc cgaaggaagg gatttttctg ccggggacta tgattgaagt cattcgggtt
     1621 ccgacgtcgc ccggcacata tcgtcatgtc ctctttgatt ttgacggtac gctgagtttg
     1681 atccgggaag gctggcccga cgtcatgatc cccatgatgg tggaatgcct cctcgaaacg
     1741 ggtactcaag agacagaaga ggaactggaa gacacggtac gtgcctttgt catggagtta
     1801 acaggaaaac aaacgattta ccaaatgatc cgcttggcgg aagaaatcaa aaagcgtggg
     1861 ggaaggccgc tcgatccgac cgagtataag cggatgtatc acgatcggct catggcgcgg
     1921 atcgctcacc ggcgggaggg tttacgttcg ggacaaatcc ggcctgaaga aatgcttgtc
     1981 cccggctccc ttgagctgct ggaagaattg cagcggcgcg gggtgcagct ctatttggca
     2041 agcggtacag acgaagttta tgtccgtgaa gaagcgaccc tgttgggact ggaccggtat
     2101 ttcggtgagc acatttatgg agccattgac gattacaaac gattttccaa ggccatggtc
     2161 attgaacaaa tcttggcgac acaccggatt acgggcaatc tcctgctcgg atttggcgat
     2221 ggatacgtcg agattcagaa tgtgaaagaa gtgggcggaa ccgccgtggc cgtggccagt
     2281 gacgaagcca aaaagagtgg ccaacccgat ccctggaaac gcgaacgttt gatcagcgtc
     2341 ggggccgatc tcgtaattcc agactaccgg gattggcaaa cgttgatcgc ttacctttgg
     2401 ggagagatca cggcgccttg tgtgccgcga gagagtcttg ggaaagaatg tgtctgaaaa
     2461 cttgagaggc caatgggaat atgggctatc cgcaatttga tcgatcgcgg ttaatcctgc
     2521 ggcctttggc cgagcgaact cacgacatcg atcgcagcat ccttttagac ccaacaacgg
     2581 tacagtcgtc atttcaccac ccggcaatcc cgacattggc ggtccggatt ctcgaggccc
     2641 gaaagcgggg cgcagccgtt atcgccttct gcggagcgca cgtcttgaga acgggaaacg
     2701 gtcccctttt gattgacctt atggaaaagg ggttactgac ccacttggct cttaatgggg
     2761 cgggggccat tcacgatttt gaattggccc tgattggggc aacgtgtgaa agtgttgctc
     2821 gttatattcg gacgggcgaa tttggcttgt ggcaagagac aggccgaatc aatgacgccg
     2881 ctatcatcgc cgcaagagaa caaatagggt tcggcgaagc cctcggacgg atgatcgaaa
     2941 ccgaacggtt cccctaccgc gagaccagcg tgttggcggc aggctatcgg ctgcgggtgc
     3001 cggtcaccgt tcacgtcgcc atcggccaag acattgtcca cgagcatccg aatttcgatc
     3061 cggtggccat cggccaggcc acttacacgg atttcctcat tttcacgcac agtgtggcca
     3121 atctcgaggg cggggtattc ctaaatattg gctcggctgt ggcaggtcct gaggtttatc
     3181 tcaaggcctt gagcatggcc cggaatgtcg cgcggcaaga gggacgcgag attcgcgact
     3241 taacgacggc cgtttttgac ctggtggcca tcgggagtga ttggcgtact gaggccccaa
     3301 agacggatcc ccggtattac tatcgcccct tcaagacggt ccttgtccgc acggttgcgg
     3361 atggtggcac gagtttctat gtccaaggag accaccgcga gacggttccc gccttgtatc
     3421 gcacactcac gactttccag aaggagctcg acacactcga cacaacagtg gagtggcggt
     3481 cacaattcga acgtcgggga gccagtcgga tgaagcaggt tgaacgattt gcgggattta
     3541 ggaaagaccg cctggaagag attctttccc gcttcaaaaa tcttcgcgtc gccgttgtgg
     3601 gtgacttttt tctcgataag tacctcgacg tcgatccccg attggcagag acgagcttag
     3661 aaaccggtaa gctagcacat caggtggtgc gggtccgcca cagcccgggg gccgcaggaa
     3721 ctattgtttg caatttagcg gctttgggaa tcggcaccat ttactgcgtg ggattgatcg
     3781 gggacgacgg tgagggttac gagttgcggc gagacttggt caacttaggc tgtcacatcg
     3841 agcatctcca cgtttgcccc gatgctatga cccccacgta tctcaagccg cgggacatta
     3901 ccgatccttc gctggccggg gagcattctc ggtatgattt caagaatcga tctcagacgc
     3961 cgcctgaact tcgccaaaag attgccaatt cgctttcgac actcctgccc caggttgatg
     4021 ccgtcattgt cttggatcaa gtcgaagaag cagactgcgg cgtggtcacg aaggacctaa
     4081 gagaactgat tgcggaactg gccccttgtc atcctggtgt gatcttttgg gcagacagcc
     4141 ggcaaagtat acgagagttt cgccacattc atcttaagtg taaccagttc gaattacttg
     4201 gaaacgcgct cgacccgtgg cggcccgtgc ccgactggga tattctcatc ccggccatgg
     4261 aggagtttcg caaggttaat aatgcaccta ttgtggtaac gctggggaaa gacggaatgc
     4321 tggtgagcga tcctgctccc acctggattc ctggggtgag ggtttccgaa ccgacagatc
     4381 ccacgggcgc cggcgatagt gccacggccg gcgctgtggc gtctctggcc gccggtgcaa
     4441 cgcttcccg
//
LOCUS       DAOPTR010000185         4418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_15969_length_4418_cov_3.916571, whole genome shotgun sequence.
ACCESSION   DAOPTR010000185 DAOPTR010000000
VERSION     DAOPTR010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15969_length_4418_cov_3.916571"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..342
                     /locus_tag="PKI05_04840"
     CDS             181..342
                     /locus_tag="PKI05_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51559.1"
                     /translation="MPRNELKLSFFFFRHVTNILFWMSFVLPPNLPTNRRPPAWAFNF
                     VILADPEGT"
     gene            703..2019
                     /locus_tag="PKI05_04845"
     CDS             703..2019
                     /locus_tag="PKI05_04845"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA51560.1"
                     /translation="MSKSRLSLDSTPSRRQFLKATTAAVSGAMVTLPLERMVHAAGQE
                     GFKIGLIGCGGRGSGAAANAMNAGKDIKLVAMADIFEEHLRSAREQLKARYPDQVQVD
                     DDHCFVGFDAYEKLINCGVDVVLIAAASHFHPVMFKAAVDAGKHIFCEKPHALDVPGL
                     KLAMAACEEAKKKNLAVVSGLCWRYDYGVREAMKRVFDGAIGEIVAIQETYITSPYVV
                     RPRQPGWSEAYYQFKNWYHFNWLSGDQTGQQLIHSLDKASWALGDKPPLRAWGLGGRQ
                     ACIDPKYGDLFDHHSVVFEYDNGVRVFGFCRDIPGCYNETSDIIFGTKGRCILPSRPR
                     IEGATEWEYKGPKVSMYDVEHKELFDSIRQGKPINNGDYMFTSTMLGILAQMVCYTGQ
                     LITWEEAMQSQLSFSLPRYGFDIDPPVKPGPDGIYPAPIPGVTKFQ"
     gene            2131..3003
                     /locus_tag="PKI05_04850"
     CDS             2131..3003
                     /locus_tag="PKI05_04850"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOA51561.1"
                     /translation="MNAPKTPLVGLTFVVVLGLLVGAKTKGDESPPIPPLDAATIEHM
                     RQVHARFDGRKGTLALFGDSITVSLAFWAPLAYECRNMPPEMERAVRVVKSYMLPECW
                     RDWRGPEFGNEGRTTIRWAFENIDGWLTRLRPETAVIMFGTNDLNDLDEQEYREKLRA
                     VVEKCLNRGTIVILTTIPPRHGRAEKAVRFAQIAREVAEQLKVPLIDYHAEILKRRPE
                     DWDGADEKFQGYETYEVPTLISRDGVHPSNPKSMMGDFSEEALRTSGFGLRNYLTVLR
                     YAEVIEKVLQVPQE"
     gene            3481..>4418
                     /locus_tag="PKI05_04855"
     CDS             3481..>4418
                     /locus_tag="PKI05_04855"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOA51562.1"
                     /translation="MLGILSMKRRGFTLVELLVVIAIIGILIALLLPAVQAAREAARR
                     SQCTNNLKQLGLALHNYHDIHKVFVALKAGTQGTDPWDDGNNTQLCGLIPLLPFLEQR
                     ALWDQIVAGGTLNNGTRVNPFGPAAWRTDYPPFRQQTPALLCPSDPAYGKQNPASEQA
                     FNNYMFSVGDHINRNAYDRPNRGPFAHQYWCSFSDIVDGSSNTIGMVERSIYTRSGAL
                     KGDGVANLSGIDQNPTLCLATKGANGMYASGYTIHSEKAVGRRWADGRPYWNGVTTVL
                     PPNGPTCLGTANSWEWGIFSPSSYHPGGVNGLLMDGS"
BASE COUNT          997 a         1201 c         1241 g          979 t
ORIGIN      
        1 gggttgcccc tacgatttag gatcgcatcg aaacctgtag gggcaggccc ctgtgcctgc
       61 cccaaagcgc aaatggcgcc gggtccgcgc aaaatccaaa caacgcggcc gccccgccaa
      121 acgcaaacga ccggcgctgc gaacaaaccg ggcaaccaca gggggttgcc cctacgattg
      181 atgcctcgaa acgagttgaa gttgtcgttt ttcttcttcc gccatgtgac caacatcctc
      241 ttctggatga gcttcgttct tccgccaaac ttgccgacga atcggcgtcc cccagcttgg
      301 gcatttaact tcgttatcct tgccgatccg gaagggacct agcagaaaag gctggataac
      361 gcgtttctat agatcaaatg tcgtcgataa tacgcaaaaa agagcgtcac gcctctcgcc
      421 gtttccatcg tttcttatct catcaccggc cccattgctt ctgcaaacct tccgtgggct
      481 gtgcctcctc tttagtgcct cgcagatgtc aaaataggta cacctcccag atagtgttcc
      541 catgttctcg gttttccggt tccacccgag atgcgggcac gccgcgtagc ttgcttttgc
      601 catgcaggtg tggcaagatg tttctggcaa atccgcccat gaaaaaccat ggacgatgaa
      661 ggttgtgctt cgccccgtct caaaaatcgg agggaaaaca ctatgtccaa aagccgttta
      721 tcgctggatt caactccgtc gcgtcgtcag tttttgaagg ccaccacagc cgcggtcagt
      781 ggcgccatgg tcacactccc cttggaacgg atggttcacg ccgcgggcca ggaagggttc
      841 aagatcggcc tcatcggttg cggaggccgg ggaagtggtg ccgcggcaaa cgccatgaac
      901 gcgggaaaag acatcaagct ggtggcgatg gccgacatct ttgaggaaca cctccggagt
      961 gcacgcgaac agttgaaggc ccgctatccc gatcaagtcc aggttgacga tgatcactgc
     1021 tttgtgggct tcgacgctta cgaaaagctc atcaactgcg gtgtggatgt ggtgctcatt
     1081 gcggcggcat cgcattttca ccctgtgatg tttaaggcag ccgtggacgc cggaaagcat
     1141 atcttctgtg agaagcccca cgccctggat gtgccgggtc tgaagctggc catggccgcc
     1201 tgtgaagaag ccaaaaagaa gaacctagcc gtcgtttccg gattgtgctg gcgatatgac
     1261 tatggtgtcc gtgaggcgat gaagcgggtg ttcgacggcg ccatcggcga gattgtcgcg
     1321 atccaggaga cctacatcac gtcgccctat gtcgtgcggc cacgccaacc tggttggagc
     1381 gaggcctact atcagttcaa aaactggtat cacttcaact ggttgtccgg tgaccaaacg
     1441 ggacagcaac tcatccacag cctggataag gcgtcttggg cccttggcga caagccaccg
     1501 ctgcgggcat ggggactggg aggccgacag gcctgtatcg atcccaagta tggcgatctg
     1561 ttcgaccacc actccgtggt gtttgaatac gacaacggtg tgcgtgtctt cgggttctgc
     1621 cgcgatatcc ctggctgcta caacgaaacg tcggacatca tcttcggaac caaagggcgt
     1681 tgcattctgc ccagccgccc ccgcatcgaa ggcgcaacag agtgggagta caaaggccct
     1741 aaggtcagca tgtatgatgt cgagcacaaa gagctgttcg actccattcg tcagggtaaa
     1801 cccatcaaca acggcgatta catgttcacg agcaccatgt tgggcatctt ggcgcagatg
     1861 gtgtgctaca ccggacaact gattacctgg gaagaggcca tgcaatccca attgtccttt
     1921 agcctgccac gatatgggtt cgacatcgat ccgcccgtca aaccgggtcc ggacggcatc
     1981 tatcccgcgc ccatccctgg cgtcacaaag tttcaatgag cattggcagc acacgttcgc
     2041 atcattcccc ggccaagaga tcgtgaaact acggtgcagg ctttcggtcc gagagcaagc
     2101 gtgcccgcgg gtcgcgcgga ggttatcgac atgaatgccc ctaagacgcc gcttgttggc
     2161 ttgacttttg tggttgtcct cgggctgctg gttggtgcca agacgaaggg cgacgaatcg
     2221 ccgccgatcc cgccacttga tgccgcgacg atcgaacaca tgcggcaagt ccatgcccga
     2281 tttgatggcc gaaagggaac actcgcgctc ttcggggatt cgattaccgt cagtctggca
     2341 ttttgggcac cgctggccta cgagtgccgc aacatgccgc cggagatgga acgggcagtg
     2401 cgtgttgtca agtcgtacat gctgcctgaa tgctggcgcg attggcgcgg ccctgaattt
     2461 ggcaacgagg gcaggacgac cattcgctgg gcgtttgaga atatcgatgg atggttgaca
     2521 cgcctgcggc ccgaaaccgc cgtcatcatg ttcgggacga atgacctcaa cgatttggat
     2581 gagcaagaat accgcgagaa gctccgtgca gttgtggaaa aatgtttaaa tcgcgggacg
     2641 atcgtcatcc taaccacgat tccgccccgc cacggtcgcg cggagaaggc tgtccgcttt
     2701 gcccaaattg cacgcgaggt tgccgagcag ttgaaagttc ccctgattga ctatcacgcg
     2761 gaaatcctca agcggcgtcc tgaggattgg gacggtgcag atgaaaagtt ccaaggctac
     2821 gagacgtatg aagtacccac cctcatctcc cgagacggtg ttcacccaag caatccaaaa
     2881 tcgatgatgg gcgacttctc cgaggaggcc ttgcgaacat cggggttcgg cttgcgaaac
     2941 tatctcacgg tcctgcgtta cgccgaagtg attgagaagg tgctgcaagt gccgcaggaa
     3001 tagttcctcc aaggctcagg gttgatattg tccctccgcc ccctactctc aagaaaaaca
     3061 gaaatgccgg gaaaaaggtc cgtcggagcg gagtgttcga ccggatgtta aggggaggct
     3121 tcccattgcg atagcgaccg aacaattttc tttgggaaca cccgcaaaat tgttgacaag
     3181 catttgtcgc cacggtagat tatagctggc tagggtaaag ctttccggct aactgctaga
     3241 ggaccactcc gtgcttcccc ccagcagcaa gcctttcata acgctgcctt aaccccactg
     3301 cgcatgtccc ctggagggtc agcttagact tcctaaagca aaagctctaa ctgcaaatca
     3361 ccagaatgtg ctatgcgtgg cggtgaataa aaaactgtag ccgcgcgtcg tggcatgata
     3421 acgtgttaaa acgtgttaat gagtggtttt gttgttatcg agaaacttag gaggtgtcct
     3481 atgctaggca ttttgtcgat gaaaagacga ggttttacgc tcgtcgaatt gctcgtggtg
     3541 attgccatca tcggtatttt gatcgctttg ctgttgccgg ccgtccaggc ggcacgggag
     3601 gctgctcgac gaagccaatg cacgaataat ttgaagcaat taggtttggc cctgcacaac
     3661 tatcatgata tccacaaggt ttttgtagcg ttgaaggccg gcacacaagg cacagacccg
     3721 tgggatgatg gcaacaatac tcaattgtgt ggtctgatcc cgctattgcc attcctcgaa
     3781 caaagggccc tgtgggacca aatcgttgct gggggcacac tcaacaacgg aacacgggtg
     3841 aatccattcg gcccggcagc ctggcgaacg gattatccgc cttttcggca acagacaccc
     3901 gctcttcttt gcccatcgga ccctgcctac ggaaagcaga atcctgcgag tgagcaggcg
     3961 ttcaacaatt acatgttcag cgtgggtgac catataaacc gaaatgctta tgatcggcca
     4021 aaccgcgggc cgttcgcgca tcagtattgg tgcagctttt ctgacattgt ggacgggtca
     4081 agtaatacga ttggaatggt cgagcgaagc atttacaccc gctccggtgc gttgaagggt
     4141 gacggcgtgg cgaatctttc gggtattgat caaaacccga ccctttgctt ggccaccaag
     4201 ggtgcaaacg gtatgtacgc tagtggttat accatccaca gtgagaaggc cgttggcaga
     4261 cgctgggcag atggacgtcc ttactggaat ggtgtgacca ccgtgctccc gccaaatggg
     4321 ccgacctgtt tgggtacggc taatagctgg gaatggggga ttttctcgcc gtccagttat
     4381 caccccggtg gtgtcaatgg attattgatg gatggatc
//
LOCUS       DAOPTR010000186         4412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_15991_length_4412_cov_3.268992, whole genome shotgun sequence.
ACCESSION   DAOPTR010000186 DAOPTR010000000
VERSION     DAOPTR010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15991_length_4412_cov_3.268992"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..241)
                     /gene="csrA"
                     /locus_tag="PKI05_04860"
     CDS             complement(23..241)
                     /gene="csrA"
                     /locus_tag="PKI05_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HOA51563.1"
                     /translation="MLVLSRKCNEQIVIGDDIIVTVVSIRGGTVRLGIEAPPHVTVHR
                     LEVLQAIRADEAARMKDSSNNSSPPTTP"
     gene            complement(441..857)
                     /locus_tag="PKI05_04865"
     CDS             complement(441..857)
                     /locus_tag="PKI05_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOA51564.1"
                     /translation="MPVYREKLRYTTQGHGHMLDLTQDVAKVCNRAGVSDGLVNLFVV
                     GSTAAIGTIEFEPGLLKDFPNLLDKLAPPGRNYAHEATWHDGNGHSHLQATLLGPSLT
                     IPLESGALALGQWQQVFLIECDVRSRQRTVIVSVLS"
     gene            complement(900..1145)
                     /locus_tag="PKI05_04870"
     CDS             complement(900..1145)
                     /locus_tag="PKI05_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51565.1"
                     /translation="MSRAFFLAIGLFVVFLGVQTLGVQRFVLKTRDPAPPGNVAIGES
                     QPGPNKVLEPPPWAPWSLMATGAIVSLYAVTLPTVRK"
     gene            1673..3307
                     /locus_tag="PKI05_04875"
     CDS             1673..3307
                     /locus_tag="PKI05_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOA51566.1"
                     /translation="MYPTLEKADHEVGLALQEAKDEGTVPVDNLTSAVATYLPQAVFI
                     VDEKFRVVWANNRASELLPAATPLVGKQFYQDLFRDVQLIRPEFFPLTLARDTGRPAR
                     SVMRLKGPRFFELFASRIPQTEEDRVRLIVSMQEITSTFLPLERLRAIHEAGRHLTEF
                     APEFVTRYSAEERKEVLKRRVLDYLKKVLHFDVFEIRLIDPQTKELVPFISDGMSPEA
                     AQRRLFVGLQGQGITGYVAATGASYICEDTASDALYLKGTEESRSSLTVPLIRHEGPS
                     RAVVIGTVNIESPRPCAFSHDDRMFLEIFCRDLVVALTTLDLIEAEANVATRGLVEAI
                     HGRVALPVDQIVSKAAEADAYLDHDPARCDVEKARAILKEIHKIARDLRTEIRSFGAR
                     LPPTEASPIFQNKRVLVVDPDESIQRDAHKYLEQWGCVVETASTGRAGIQMAVMTAGE
                     GGYDVILGAHRLPDMKGWEFFKQLKEALKAEKLPYVMLCPIGQHDADHNFVKARELGA
                     EYQVGKQPPPPGQTSGGNLVVKPLVNILNRIFGVTT"
     gene            3432..>4412
                     /locus_tag="PKI05_04880"
     CDS             3432..>4412
                     /locus_tag="PKI05_04880"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOA51567.1"
                     /translation="MEILAPANDFACLEAAVEAGADAVYFGLPHLNARRRARNFTPDE
                     AARAVQFLHSHGRKAFLTLNTDISYRELGLALSTLEQANEIGFDAVLVRDPALLALRP
                     AYPKLEFHFSTQTTIANSVDVEAARELGADRVVLARELDLDEIRAACSVPGIAVEVFA
                     HGALCFSISGRCLMSSWVGGRSGNRGVCASPCRVPWQVDGEPAGFPLSMHDLCILEHL
                     SELSAAGVSALKIEGRLKSAQWVKRAVSLYRQALDDTADKNSLLSLAKALTEYAGRDL
                     TDGYLLGRRTNLTGTNRGRVGRDETSLIEEDGDLADSTTDPTQLSRPGHVV"
BASE COUNT          985 a         1119 c         1304 g         1004 t
ORIGIN      
        1 tccgaaaacc tcacgagaca gctcacgggg tggtgggagg ggaagaattg ttgctgctat
       61 ctttcattcg cgcagcctcg tccgcacgaa tcgcctgaag gacctcgagc cggtgaacgg
      121 tcacgtgtgg cggtgcttca atgccgaggc gcacagtccc cccccggatc gacacgacgg
      181 tcacgatgat gtcgtcgccg atcacgatct gctcattaca tttccgcgat agaaccagca
      241 tatgcccttc cgtcgccccc aaatccaact gaatgcactg ctatcatatt atacctggtt
      301 gccggaaaaa agaaacaaaa aagcttttat ccccccttct caaggaaatg attccgcccg
      361 cttacgcaaa acgtctgcgg gatcggtcct cgaaatgatg aactgttttg aaaatccgga
      421 tggcccccga agagagggag ttagctcagc acgctgacga tgaccgtgcg ctgccgggac
      481 cgcacatcgc actcgatcag aaacacctgt tgccactgac cgagggccag agccccggac
      541 tccagtggaa tagtgagaga ggggccaagt agcgtggcct gcaaatgcga atggccatta
      601 ccgtcgtgcc aggtcgcctc gtgggcataa ttgcgaccag gtggagctag tttgtcaagc
      661 aaattgggaa aatctttgag taagccgggt tcaaattcga tcgttccgat ggctgcggtg
      721 ctacccacta cgaaaagatt gaccaagccg tcggaaactc ctgctcgatt gcagactttg
      781 gccacgtcct gggtcaggtc aagcatatga ccgtgcccct gtgttgtgta gcgaagcttt
      841 tctcggtaaa ccggcataaa cggacaaccc gctcttgtgg tgagggcgaa ttaaggggct
      901 tatttacgca cggtggggag tgtcacggcg taaagcgaga cgatagcccc ggttgccatt
      961 aagctccacg gtgcccaggg gggtggttcg aggaccttat tggggccagg ttggctttcg
     1021 cctatggcca cattaccagg gggggctgga tcccgcgttt tcagcacaaa tcgctggacc
     1081 ccgagggttt gaacccccaa gaagaccaca aacagcccaa tggccaggaa aaaagcgcgg
     1141 ctcatctggc gcgtgcccaa caaaatcacg atcgcgggaa ctaacaatga tcctcaatga
     1201 ttgaaatcgt ctcggtgata gcagccagat aaaagcggat cacaagagat gggttggaat
     1261 agttgggcaa attgcaacaa agaaaccacc gccgtgcgtc acgccgtgct gtgccacagt
     1321 ttgaaacgcc ggctcactag tgtggtcacg aaaccgtttc gcgcgttgat tggtcgagaa
     1381 gattcttggc gagctcgttt ggatacaaac gaatcaagta tttttaattt ctacacttac
     1441 gttcattgcg cctacaccag attgtggcag ggcggaacgc cgcggccatg tggtccgcat
     1501 cgcaatcgtt tgccacagta gttgacaacc gtggcgcggc gcctacaata tgttggggag
     1561 ttagcggcta tcgtgatgca gcgagttgcc ctcttagtgg ttacatcgct atggcgttgc
     1621 gaatcagcgg gtgatggccc actgttgcgc ccgatgacag gactaaacaa tcatgtatcc
     1681 tactcttgaa aaggcggatc atgaagtcgg cttagcttta caggaagcaa aagacgaggg
     1741 gactgttcct gtggataatc ttacctccgc ggtcgccaca tatcttcccc aggccgtctt
     1801 tattgttgac gaaaagtttc gggtggtttg ggcgaacaac agggcatcgg aactgctgcc
     1861 tgcggcgact ccattagttg ggaagcaatt ttatcaggat ttgttccggg atgtgcagct
     1921 catccggcca gagttctttc ccctgacgct tgcacgtgat acaggacgcc cggcgcgatc
     1981 cgttatgcgt ctcaaaggcc cgcggttttt tgagctgttt gcgtcacgga taccccagac
     2041 ggaggaggat cgagtccgtc tcatcgtaag tatgcaagag ataacgtcaa cctttctccc
     2101 tctcgagagg ttgcgggcaa ttcatgaggc aggacggcac ctgacggaat tcgcgcccga
     2161 atttgttaca agatacagcg cggaggagcg aaaagaagtc cttaaaaggc gggtcctgga
     2221 ttatctcaag aaggtgttgc acttcgacgt ttttgaaatc cgattgattg acccccaaac
     2281 caaggagttg gtccccttta tctccgacgg aatgagtccg gaggcggccc agcgacggct
     2341 cttcgttggt cttcagggcc aaggaattac tggatatgta gcggcaaccg gggcgagtta
     2401 catctgtgag gataccgcca gcgatgccct ctatctgaaa gggacggagg agtcgcggag
     2461 ctctctgacg gttcctctga tccgtcacga agggccttcg cgggctgtgg tgattggcac
     2521 agtgaatatc gaaagtccgc ggccctgcgc attctcccat gatgatcgaa tgtttctgga
     2581 aatcttctgc cgcgatcttg tggttgcact gaccacgttg gacttgatcg aagccgaggc
     2641 aaatgtggcg acacggggcc ttgtcgaggc gatccatgga agggttgctc tgcccgtgga
     2701 ccaaatcgtt agcaaagcgg cggaagccga tgcttatctc gatcatgatc cggcacgctg
     2761 tgacgtggaa aaggccaggg cgattctcaa agaaatccac aagattgctc gcgatttgcg
     2821 aacggagatc cgctcgtttg gggctcgatt accaccgact gaggcctcgc ctatttttca
     2881 aaacaaacgc gttttggttg tggatcctga cgagtcgatc caaagggatg cccataagta
     2941 tttagaacaa tggggatgtg tggtggaaac cgcttccaca ggacgtgcgg gaatccagat
     3001 ggccgtgatg acagcaggtg aggggggata cgacgtcatt ttgggtgccc ataggcttcc
     3061 cgatatgaag ggctgggaat tctttaagca gctcaaagag gctctgaagg cggagaagct
     3121 gccctatgtc atgctctgtc cgattgggca gcacgatgcg gatcacaact ttgtaaaagc
     3181 acgagagctc ggcgcggaat atcaggttgg gaagcaaccg ccacccccgg ggcagacttc
     3241 tggtgggaat ctcgttgtga agccgttggt gaacatcctt aacaggattt tcggggtcac
     3301 gacttaaaat gcggtcgtct gtcgagctgg aatgaatatg tccaggtgtt ccgggtaaga
     3361 ataagataac ttaaaggggc gctgtcgtct gcttctattt tcggtgaagc caaagattgc
     3421 tgagcggttt aatggaaatc ctcgcacctg cgaatgattt cgcgtgtctg gaggcggctg
     3481 tcgaagcggg cgcggatgcc gtctattttg ggttgccaca cctcaatgcc cggcggcgcg
     3541 ccaggaattt cacacccgac gaggctgccc gagcggtgca attcttgcac tcccatggcc
     3601 gaaaggcatt tctcaccctt aatacggata ttagttatcg ggaactcgga ttggcgctga
     3661 gcaccttgga gcaagccaac gagatcggct tcgacgcggt gctcgtgcgt gatccggcct
     3721 tgttggctct gcggcctgcg tatccgaagt tggagtttca tttcagcacg caaacgacca
     3781 ttgccaacag tgttgatgtt gaagcagcac gcgaacttgg tgccgaccgc gtggttttgg
     3841 cccgtgagct tgatttggat gaaatccggg cggcttgttc ggtgccgggg attgccgtcg
     3901 aggtgtttgc ccacggggca ttgtgctttt ctatttccgg gcggtgtttg atgtccagtt
     3961 gggtcggtgg gcgtagtggg aaccgggggg tttgtgccag cccatgtcgt gttccctggc
     4021 aagtggatgg cgaaccggcc ggttttccac tttcaatgca cgacttgtgt attttagaac
     4081 acttgtcaga actctctgcg gctggtgttt cggcgctcaa aattgaggga cgactcaagt
     4141 cggcccagtg ggtaaaaagg gccgtaagtt tgtaccggca ggcccttgat gatacagccg
     4201 acaagaactc actactgtct ctcgctaaag cgttgacgga gtatgctggc cgcgacctta
     4261 cggatgggta ccttctcggg cgccgtacga atctcactgg gacaaatcga ggccgggtag
     4321 gtcgggatga gacatcgcta attgaggagg acggggatct ggccgattcg accaccgacc
     4381 cgacgcagct gtcccggccg gggcacgtgg tc
//
LOCUS       DAOPTR010000187         4406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16022_length_4406_cov_2.978396, whole genome shotgun sequence.
ACCESSION   DAOPTR010000187 DAOPTR010000000
VERSION     DAOPTR010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16022_length_4406_cov_2.978396"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PKI05_04885"
     CDS             complement(<1..682)
                     /locus_tag="PKI05_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOA51568.1"
                     /translation="MKIWFNRINESHRTMIEVDAAEVTIGRDASNTVVLASPLVSRRH
                     AIVRCLEDGRLQLENLGLNSCLVGDEEVLGGQTVVFSPGTRVRIWPYTLTFEAEKAVN
                     ISRQELEAHLRSLVADLELRIHRKLLERLDLYEFENRPTNDPQSILLLENNIEDICRE
                     LRVFEPENEPILEEITGLMLRDYLVNSLIMESGKDSLFEMTGLNANEFDIPATLVPER
                     ETELAGILN"
     gene            complement(753..1523)
                     /locus_tag="PKI05_04890"
     CDS             complement(753..1523)
                     /locus_tag="PKI05_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51569.1"
                     /translation="MKGIHGVVLGVAVGIAAALFNWAYITRATGDIDAVAFVGIAPGK
                     DLEAGHILAEEDLVPVPIPAIPARRLKDFGYLYADRASIVGLRITRPIPGGSLLLQDD
                     LRPPPPKLRFGGQPVGNTEEIAMFIPIDVRTIVPSLLEPGDMVSFIVGGPTPTAGVGN
                     PDPAAGGEGSAAGSSLSGGPQVIGKFKVLAIGNRLSSVEVHRAYQLPQGQENILTISV
                     RLEGGRLEPKAERLWKMLELTNFRQVGVILHSREASGN"
     gene            complement(1672..2775)
                     /locus_tag="PKI05_04895"
     CDS             complement(1672..2775)
                     /locus_tag="PKI05_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51570.1"
                     /translation="MAGDHPSHPEIPTELERKDSPQTDGVNLSPSRALAVDSELGWSK
                     LAKRITGWTSNLLVTFLILAAGLVFGVQVLLWWKDTATEDQTTVNRDTRESLQVSRRW
                     AEFYGGGGGFSCISFQGEEKAAIRRCEETCLEAVQRAVVRPGLPDAAERRVLAKLASI
                     LLIREEPNGSRVYRYPGDVPLWVGVKLGEKKSEGTDRAGAVDTDATDAGSLPPGSRIV
                     AWAFLMPVTEREWTIYLLSPAAEGPQKGTLGSLGEEEFLFPVPPGATVVSRLSPGEGA
                     FFGAFEESTPGASVTWPLFLDRELERQGWVPVGDWLGGAARKRRVFGRGTQTTEQSFL
                     SIEITQDAHRPARGLVFGFRWTTEKRERSSEPQ"
     gene            complement(2765..3832)
                     /locus_tag="PKI05_04900"
     CDS             complement(2765..3832)
                     /locus_tag="PKI05_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51571.1"
                     /translation="MTRFSPPRWAAEVNSNESLPRGGTVFGKRGLAPLEFVIAVPLFL
                     FIMALMINFGTVSAWRVRGLAVARQAVWADRPPRNGGTVPRPDYWPIPANLGAGADYD
                     APVLDDPRVNLPVARGPTLGTFVVNDHLLDPAKGFRRGTSEIERSFPMLASLGGYQLS
                     SRAPILDNCWRYRQTGLPYWWHDHWAHRVTALYRLPTAGGNYLALYVQAALAILRMPQ
                     RNDLLILDRDPEFPAYAARFGWRGGGSPDFHPGLSRFCSLDSSLAQERVENLIDRIAG
                     VAPKQGPPQVNHVPSLAERMAGAYINLYRRVIQELENQLNAVPPPSASQIAAIQAEIA
                     DLQQKIDTLEAFRQSLQNHGR"
     gene            complement(3879..>4406)
                     /locus_tag="PKI05_04905"
     CDS             complement(3879..>4406)
                     /locus_tag="PKI05_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51572.1"
                     /translation="YPASNLPFVIQTPGDEVVDPNSHLDQYFTFVGVAYWRPMRATLP
                     GLFQHPMGSDTIAYAQVRVFIPRARLIWHYYVDTPENILGGIPGDFAELPPDDAEQSE
                     PAQGEWRVVREDVPTHWDLLNQNWTCALQPATVWSLPLIVQTRPPLPEFAGWNVQLPS
                     LPGWTTADIQYVSPH"
BASE COUNT          994 a         1405 c         1120 g          887 t
ORIGIN      
        1 aattaagaat acccgccagt tccgtctctc gttctgggac gagtgtcgcc gggatgtcga
       61 actcgttggc atttaagccc gtcatctcaa agaggctatc cttgcccgat tccataatga
      121 ggctattgac aaggtaatca cggagcatca atcccgtgat ctcttccaag atcggctcgt
      181 tttctggctc aaagactcgc aactcgcggc aaatatcctc gatattgttt tccaaaagca
      241 agatgctctg cgggtcgttg gtgggccgat tctcaaactc gtacaggtcc agtcgctcca
      301 gcaacttccg atggatgcgc aattcgaggt cagccaccag ggagcgaaga tgggcctcca
      361 attcttgccg cgagatgttg acggccttct cggcctcaaa tgtcaaggtg taaggccaaa
      421 tccgcactct tgtgcccggt gaaaaaacaa ccgtctgccc gcccaaaacc tcctcatcac
      481 ccaccaggca actgttaagc cctaagttct ccagttgtaa acgcccgtct tccagacaac
      541 gcacaatggc atggcgacgc gagacgagtg ggctggccaa aacgacggtg ttcgaggcat
      601 cccggccaat ggtaacctcg gccgcgtcca cctcgatcat cgtgcggtga ctttcgttga
      661 tccggttaaa ccagattttc atgagctgca acccattctc tgttgcccgc gaaacggtgc
      721 gtcctgcata cccgttcgat cattcgatca cgtcaattgc cagaggcttc ccgcgagtgt
      781 aggataacac ccacctgtcg gaagtttgtc agctccaaca tcttccaaag gcgttcggcc
      841 ttaggctcca ggcgtccacc ttccaaacga acgctgatgg tcaggatgtt ctcctgtccc
      901 tggggcaact ggtaagcccg gtgaacctcc acgctgctca agcggtttcc gatggccaag
      961 accttgaact taccgatcac ttggggacca ccggacaacg aactgcccgc ggcggaaccc
     1021 tccccgccgg cggccggatc gggattaccc acacccgcag tgggggtcgg gccacccaca
     1081 ataaacgaca ccatgtcacc cggttccaac agcgacggaa cgatcgttcg cacatcaatc
     1141 gggataaaca tggcgatttc ctccgtgttc cccaccggtt ggcccccgaa ccgcaacttc
     1201 ggcggcggtg gacgcaggtc atcctgcagg aggagcgaac caccagggat gggacgtgta
     1261 atccgcaatc ccacaatact cgcccgatcg gcgtataaat agccaaaatc tttcaagcgt
     1321 cgtgccggta tggccgggat cggcacgggc acaaggtctt cctctgccaa gatgtgaccg
     1381 gcctcgaggt ctttgccggg tgcaatcccg acgaaggcaa ccgcgtcaat atcacccgtt
     1441 gcccgagtga tgtaagccca attgaaaagt gccgcagcaa tccccacggc cacgcccaag
     1501 acgactccgt gaatcccttt catcgcctat cgctctcaca cttcgcgagt ctcggaggac
     1561 atttgctccc tacgtcaatg actaaacccg gtttacgaac tacgcgtagc cctccttttc
     1621 cattctaacc gcaaaccacc agccctgccc cctgttccaa tcacaagcca tttactgtgg
     1681 ttccgaactt ctctcccgct tctccgtggt ccaacggaac ccgaacacca aaccacgtgc
     1741 cggtcggtgg gcgtcttgag ttatttcgat gctgagaaaa ctctgttcgg tcgtttgagt
     1801 gcctcggcca aaaacccggc gtttcctcgc cgcccccccc aaccaatcgc cgaccggcac
     1861 ccaaccttgc cgctccagtt ctcgatccaa aaaaagcggc caggtcacag aggcccccgg
     1921 cgtggactct tcgaaggccc cgaaaaatgc gccctcgcct ggagacaacc tcgaaacaac
     1981 agtcgccccc ggcgggacag gaaacaaaaa ctcttcttca ccaagcgacc caagtgttcc
     2041 tttttgtggc ccttccgctg ccggtgaaag cagatagatc gtccactccc tctccgtgac
     2101 cggcattaaa aaggcccagg ccacaatgcg gctcccaggg ggaagtgaac ccgcgtcggt
     2161 tgcgtcggtg tccactgcac cagcccgatc tgtaccttca ctttttttct ccccaagttt
     2221 gactccgacc caaaggggaa cgtctcccgg atatcgatac acccgactgc cattgggttc
     2281 ctccctgatc aggaggatac tagccaactt tgccaagaca cgcctttccg ccgcgtcagg
     2341 cagcccgggg cgcaccaccg cacgctggac cgcctcaaga catgtttctt cacaacggcg
     2401 gatcgcggcc ttttcttcgc cctgaaatga gatgcaagaa aaaccgccac cgccaccgta
     2461 aaactccgcc catcttctgg agacctgcag gctttctctg gtgtctctgt taacagtggt
     2521 ctgatcttcc gtggccgtgt ctttccacca caagagcacc tgcacgccaa acaccaggcc
     2581 cgcggctaga atcaaaaatg ttacgagcaa attggaggtc caaccggtga tccgcttggc
     2641 caatttgctc caccccagct ccgaatcaac cgccaacgcc ctactcgggg aaagattcac
     2701 gccatcggtt tggggagaat ctttccgctc caattcggta ggtatctcag ggtgagacgg
     2761 atgatcaccg gccatgattc tgcaagcttt gccgaaacgc ttcgagagtg tcaatttttt
     2821 gttggagatc ggcaatttcg gcctggatcg ccgcaatctg actggccgat ggtggtggga
     2881 cggcgttcaa ttggttttcc aactcctgaa tcacacgccg atacaaattg atatacgccc
     2941 cggccatccg ttccgccagc gatggtacgt gattcacctg cggcggacct tgcttcggcg
     3001 ccaccccggc gattcggtcg atgaggtttt cgacccgctc ttgggccaag ctcgaatcca
     3061 gcgaacaaaa gcgactcagt ccgggatgga agtccggtga tcccccaccc cgccagccaa
     3121 agcgggctgc ataggccggg aattccgggt cacgatcgag aatcagaaga tcattccgtt
     3181 ggggcatcct caggattgcc aaggccgcct gcacgtataa cgccaagtaa ttcccaccgg
     3241 ccgtgggcaa tcgatacagg gccgtcacgc ggtgcgccca atgatcgtgc caccaatacg
     3301 gcaagcccgt ctgccgatat cgccaacaat tatccaggat tggagctctt gaactcagtt
     3361 gataaccccc aagactcgcc agcatcggga aagatcgttc aatctcggac gtgccccgcc
     3421 gaaagccctt cgcaggatca agcaaatgat cgttgacaac aaaagtgccg agggtcggtc
     3481 cgcgagccac aggcagattg acgcgcgggt catcgagcac aggtgcgtcg taatctgctc
     3541 ccgctcccaa attcgcagga ataggccagt aatcaggccg ggggaccgtt cccccattgc
     3601 gcggagggcg atccgcccac accgcttgcc gggccacggc caggccgcgg acccgccagg
     3661 cagaaaccgt cccaaaattg atcatcaacg ccatgatgaa caagaacaac ggcacagcga
     3721 tgacgaattc gaggggggcc aggccgcgtt tgccgaagac cgtgccgcca cgggggagac
     3781 tttcgttgga attgacctcc gcagcccatc gcggtgggct aaacctggtc acctttcctt
     3841 tgccggttcg aaccatacac gcacgctgtt gcaatacctc agtgaggact cacgtactgg
     3901 atatccgccg tcgtccaccc gggaaggctg ggaagctgca cattccatcc ggcgaattcc
     3961 ggaagaggcg gccttgtttg gacaatgagc ggcaaactcc acacagttgc cggttgcagg
     4021 gcacacgtcc aattctggtt gagcaaatcc cagtgcgtgg ggacatcctc tcgaaccacc
     4081 ctccactcgc cctgagcagg ttcggattgc tccgcgtcat ctggtggaag ctcagcaaaa
     4141 tcgccaggaa tcccgcccaa aatattctca ggtgtgtcca cgtaataatg ccatatcagc
     4201 cgggctcgtg gaatgaacac ccgcacttga gcgtaagcga tcgtatccga tcccatggga
     4261 tgctgaaaca atcccggcaa cgttgccctc atcggacgcc agtaggccac gccaacaaac
     4321 gtgaaatact gatcgagatg ggaattcgga tcgacgacct catctcccgg tgtttgaatc
     4381 acaaacggca aattgcttgc cgggta
//
LOCUS       DAOPTR010000188         4398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16052_length_4398_cov_3.403408, whole genome shotgun sequence.
ACCESSION   DAOPTR010000188 DAOPTR010000000
VERSION     DAOPTR010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16052_length_4398_cov_3.403408"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3649)
                     /locus_tag="PKI05_04910"
     CDS             complement(<1..3649)
                     /locus_tag="PKI05_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51573.1"
                     /translation="MASNVTYPRPLPPPPPVTAGRAAQHVDREELLILQQVEATRRRI
                     ATVELTWRILVLVTGTVLFAIIAAILDQWVFPKGLPPLFRWSLWATFITAASVWTVVS
                     IVPYLVRRIHPVFAAYVVEQAVPNLKHSLISFLTLRNAREEFQSDPIRKNVFRGLEAT
                     TANTIAKVPADAVVDRSQIIRTGYVLIALTAIFCLYVIFSPKDPFRSFARALLPWSRI
                     SPPTRVKIDDVRPGTSGIFEGERVVVTANVRGLKAGEEVKLVYSTSDKQAINQAIPMV
                     RERENDDRFSCELSQSFQQETTYRILAGDATTEEYRLTVVIPVRIRIRTVEYQFPRYT
                     GLAPRVTKNLGDIRAIEGTQINMTVESDVPLSTAEMVIEKLQTVSVPMAVKGREAFAR
                     TTLRLSKPEASEPEFAAYFIRGQSADGRPSVDPVKYTIEIIPDRPPEGSWLEPKEEEV
                     NLPVNGALPLRIRAVDPDFGLRHVTLEIEAASSPVAKEGLLEKPSGESHSGEFQGEYL
                     FSPKKLNLAVGDVAICRVTLVDNREPTPNRTVLPVRRIRIVAEDAAGQSSDRQPMSQP
                     QQSRNQPSQKTPDAEKNQQRPPENPAQSQLPEEKTSTANEPQSTEGPQQPREPQEQSS
                     RPAEHPAATQDRQEGASRPTPPTENPSETGEPQKNTGAEANNPQKSDPTSGSSSPQTG
                     DQGQMGDQSSSQQGKQQTGTQSPTESSQGRETGQQSETPSGEGRSGDQQRDSASTPAA
                     QQGTETGRESAGEPAKSGAGQQTDAREATSQPSADPDNRTTPSEKPGEGQQPATGSRD
                     THTPRPDGQGQTGQSEASPDRSANQGEKAGGQQQGIPTSPQTGTGSDSTASGRPQPNQ
                     GQGSGDNSAGSGERTGGDQTSGSPGVSGSPSPSEPRTTSPGTRQEQTPEQKGTPPGEK
                     VDGISNPGEAVERILSYLEKKGIDPSQLTREAKAGESEQASPREVQKGPTQGEKSRTP
                     SPTDAAGGASSPQPTPEQGGQARPGASPDMPSGVPQLTENPPASGRQDEPAAPPPMGA
                     PPGESGSPDRDPAGQGSQEPPAAGQPDSSARQNPHQPPQGVAGDQAGMGGQGGGRQSP
                     QPGEGTPGSQTPTDSQEGAPMASSEGQETGQAGAKSGASAQSNTTGQGEGGPSQEGQT
                     SQSPGTTGPADQGSRGTPSQMAGGPPSRGGMPGGTRGPGGGTPPVETPVDDPNLEFAR
                     RQTDLVLRYLEE"
     gene            4178..>4398
                     /locus_tag="PKI05_04915"
     CDS             4178..>4398
                     /locus_tag="PKI05_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51574.1"
                     /translation="MLRYGKKRLEIGKTGHPLVGHLAVLAILCLPAHVMAQGIPQGFQ
                     AQTPTGNAAPGYPNYRSQPGPQPVPGSTVS"
BASE COUNT          802 a         1252 c         1304 g         1040 t
ORIGIN      
        1 cttcctccag atagcggagg accaaatcgg tttgtcttcg cgcgaactcc agattgggat
       61 catccactgg tgtctccaca gggggcgtac caccacctgg accacgagtg ccacccggca
      121 ttccaccccg agaaggcgga ccacctgcca tttgcgacgg tgtccctctc gaaccttgat
      181 cggctggtcc tgttgttccc ggactttggg aggtctgtcc ttcttgcgaa ggacctccct
      241 caccttgtcc cgtggtgttc gactgagcgg aagcaccgga tttcgcgcca gcttgaccag
      301 tttcttgtcc ctcggagctt gccatgggag cgccttcctg agagtcggtc ggggtctggg
      361 aaccgggcgt gccctcaccc ggttgaggtg attgccgtcc accgccctga ccgcccatac
      421 ccgcctgatc gcccgcaacg ccttgtggcg gttgatgggg gttttgtctg gcggaactgt
      481 ctggctggcc agccgccgga ggttcctgcg acccctgtcc ggcaggatca cggtctggcg
      541 atccgctttc ccccggaggg gcacccatgg gtggaggtgc ggcaggctca tcttgacgcc
      601 cggatgctgg tggattttca gtcaactgcg gcaccccgga cggcatatcc ggactggccc
      661 cgggccgtgc ttggccacct tgctcgggtg ttggttgcgg agaactcgca cctcccgcag
      721 catctgtcgg cgatggtgtc cgtgatttct ctccttgagt ggggcctttt tgcacctctc
      781 gcggtgaggc ttgttcgctt tccccggctt ttgcttcccg ggtcaactga gagggatcaa
      841 tccccttctt ctccaggtaa gaaagaattc tctccactgc ttccccagga ttgctgatgc
      901 catcaacttt ttccccggga ggagtgccct tttgctcagg cgtttgttcc tgtcttgtcc
      961 caggggaggt tgtcctcggc tcagacggcg agggggaacc actcacaccg ggcgagccgg
     1021 aggtctggtc accgccagtc cgctcgcctg aacccgcaga attgtctccg cttccctgac
     1081 cttgattagg ttgaggacgt ccggatgcgg tggaatccga gccggtccct gtttgaggac
     1141 tcgttggaat tccttgttgc tgtccgccgg ccttttcacc ctgatttgca gaacgatcgg
     1201 gcgatgcctc gctttgaccg gtttggcctt gaccgtcggg acgcggcgtg tgagtatctc
     1261 gcgaacccgt tgccggttgt tggccttcgc caggtttttc cgatggcgtg gtccggttgt
     1321 ccggatccgc ggagggctga ctagtcgcct cgcgtgcatc tgtttgctgg cctgccccac
     1381 tctttgctgg ttcgccagcg ctctcgcgac ctgtttcggt tccttgttga gccgcgggag
     1441 ttgatgccga atcgcgttgc tgatcgccag atctcccttc accggagggc gtttcggact
     1501 gttggcctgt ctcacggccc tgtgaagact cagttggaga ctgtgtccca gtctgctgct
     1561 tgccttgttg tgaagacgac tgatcgccca tttggccttg atcaccggtt tgcggagacg
     1621 atgacccgct cgtcgggtca gatttctggg gattattggc ctctgccccg gtattctttt
     1681 gcggttcgcc agtttcgctg ggattctctg tcggcggcgt tggtcgcgag gccccctctt
     1741 ggcgatcttg agttgccgcg ggatgctcag ccgggcgtga cgactgttct tgtggctcgc
     1801 ggggctgctg gggaccttcc gtggactggg gctcatttgc agtcgatgtt ttttcttccg
     1861 gtaactgcga ctgagcaggg ttttcaggag gacgctgctg attcttttca gcatcgggcg
     1921 tcttctgcga tggctgattc cgcgattgtt gcggttgtga catgggctgc cgatcgcttg
     1981 actggcctgc cgcgtcctcc gccacaatac gaatccgacg aacgggcagg acggtacgat
     2041 ttggtgtggg ttcccgatta tccacaagag tcacccgaca aatggccacg tcccccacgg
     2101 ccaaattgag cttcttcgga gagaatagat attcgccctg aaattcgccg ctgtgacttt
     2161 ccccacttgg cttttcgagg agtccttctt tcgctacggg agatgacgct gcctcgattt
     2221 ccagcgtgac gtgacggagt ccgaagtcgg gatctactgc ccggatacgc aacggtagag
     2281 ccccgttcac tgggagattg acttcttctt ccttaggttc caaccaggat ccttcgggcg
     2341 gtcgatcggg gataatctcg atagtgtatt tgacgggatc gacgctagga cgcccatcgg
     2401 cactttggcc gcgaatgaag taggcggcga actcaggctc acttgcttca ggtttggaaa
     2461 gccggagggt cgttcgggca aaggcctcgc ggcccttgac ggccatgggg acacttacgg
     2521 tctggagttt ctcaatcacc atttcggcag tactcagggg gacatcgctt tcgaccgtca
     2581 tgttgatctg ggtcccctcg atggcccgaa tatcccccag gttcttggtc acacggggtg
     2641 ccaggccggt atatcgtgga aattggtatt ccacagtgcg aatgcggatc cgcaccggga
     2701 tgaccacagt cagccggtat tcctccgtag ttgcatcacc ggccaaaatg cggtaagttg
     2761 tctcctgctg gaagctctga ctcaattcgc acgagaagcg gtcatcgttc tcgcgctctc
     2821 ggaccatggg aatggcctgg ttaatggcct gcttgtctga tgtcgaatag acaagtttga
     2881 cctcttctcc tgcctttaga ccgcggacgt tagcagtgac gacgacgcgt tcgccttcga
     2941 aaatgcctga ggttcccggc cgaacgtcgt caatcttcac tctcgtgggc ggagatatgc
     3001 ggctccaagg cagtaaagca cgggcgaagg accggaaagg atccttcggc gaaaagatca
     3061 catataggca gaagatggct gtcaaggcaa ttagcacata cccagtccgg atgatctgcg
     3121 aacggtcaac gacggcatcg gcaggtacct tggcaattgt gttggccgtc gttgcctcca
     3181 gtccgcgaaa gacatttttg cgaatagggt cagactgaaa ttcctccctt gcgttgcgga
     3241 gggtcaaaaa gctgatgaga ctatgtttca gattgggcac tgcctgttcg accacgtaag
     3301 ccgcgaagac agggtgaatg cgacgcacca gatacggcac gatcgaaacg acggtccaaa
     3361 cagacgctgc cgtgataaac gttgcccaaa gtgaccatcg aaacaagggc ggtaatccct
     3421 tggggaacac ccattgatct aagatggccg caatgatggc aaaaaggacc gtaccggtca
     3481 cgagaaccaa aatccgccac gttagctcca cggtggcgat ccggcgtcgc gtggcctcca
     3541 cctgctgaag gataagcaac tcctcgcgat ccacatgctg agctgcacgc cccgcagtga
     3601 cgggaggtgg gggtggaaga ggtcgcggat aggtaacatt actggccatg attaacctgt
     3661 attgtgtaat cgctccgacc gtgccgcgtg ggcgtagcct ccagaacgat gagaagaggc
     3721 aaaccgaatg ccaccgtctc gcatctactt gtaattatag ccaaaatggg gccaggcgta
     3781 gttccgccgg gtgtccacgc ggagatgcaa aattggtacc cgtcgatgtg ggagacgcgc
     3841 cgtgccgtca catcttcaag acaacgcagt tgtagtcctt tgcgcatcta gtgaccgcgg
     3901 attgtggttc acctttattc cgtaagcgga acggacgtga atccggtgtg tggtcaataa
     3961 tcagagagct acccctattt tgctcttggt ggttctgaac ctggacttct aaaatacgga
     4021 aaatcctggg attcccctgc ctttgtccag ggagaaacag gccctgattg gcttggaggc
     4081 ggtgatctgg gcggaatgcg aaagccgggt aggggctcgc ctccgactgg atcccgcata
     4141 atgcgtggaa agtaactgaa ttgcggagac acaacgcatg cttcgatatg gcaagaaacg
     4201 cctagaaatc ggcaaaactg gccacccgct cgttggtcat ctcgcggtcc ttgccatcct
     4261 ctgtttgcct gcacatgtca tggcgcaggg gattccgcag ggatttcagg cacaaacacc
     4321 gacgggaaat gctgctccag gttacccaaa ctatcgatct caaccaggac cgcagccggt
     4381 gccgggcagc acagtttc
//
LOCUS       DAOPTR010000189         4395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16063_length_4395_cov_3.646544, whole genome shotgun sequence.
ACCESSION   DAOPTR010000189 DAOPTR010000000
VERSION     DAOPTR010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16063_length_4395_cov_3.646544"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(531..1673)
                     /locus_tag="PKI05_04920"
     CDS             complement(531..1673)
                     /locus_tag="PKI05_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOA51575.1"
                     /translation="MCLESCGSRPGIQSLPHYLGRLGYRVGLAGKVHVTPKEVFPFEN
                     VPGFCPNCTADPTPPHDLAGIREFMTRKKDQPFCLVVALVEPHVPWVMGDRSRYPVKK
                     LRLPPHIADTPKTREDFASYLAEITYMDGQVGEILGVLDEAGLREHTLVLFTSEQGAQ
                     FPGCKWTNWDLGVHTALIARWPGRVPAGKRTPAIVQYADVVPTLIELAGGQPKEMGDF
                     DGTSFVEVLLGRKDSHRTYAYFVHNNFPEGPPYPIRSVSDGRWHYIRNLTPQEMYIEK
                     HLMGMLGGSVPHNAYWPTWIAHSWNRPDVYRLVKRYCVRPAEELYDTSADSYEMTNLA
                     DRPEVAEIKKRLSEELDRWLAEQKDPGISVDTPEAYQAMKDGKPQF"
     gene            complement(1571..1945)
                     /locus_tag="PKI05_04925"
     CDS             complement(1571..1945)
                     /locus_tag="PKI05_04925"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA51576.1"
                     /translation="MRAALLLLGILAVGVVELSAVAADRPNVLIIMADDCTYNDLPVY
                     GGENAYTPNIDRLAKEGLVFDRAYLAMAICQPCRSELYTGLFPIRNGCAWNHAAPALV
                     SRVYPITLVVWVIGWDLPVRYT"
     gene            complement(2111..>4395)
                     /locus_tag="PKI05_04930"
     CDS             complement(2111..>4395)
                     /locus_tag="PKI05_04930"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOA51577.1"
                     /translation="AIVTTREIHNRLVAARAKLSPLLLAATSYCERTGRWEDALRAAE
                     GTMPEILTGIAAGNLSLVMEKLPNGLKGPVAYDVRQDRLFVREDLPLFCIRARHAVSK
                     EELVLSADAGWNKVTCQLGDGHSSLKVRWQDFSDARLKGIAVEVEAIAEESRQAIRWR
                     LKVENESTEWSIWSVVFPQVAIAEPGRDAWVVYPKGVGIAEQGAWERALQFRGRYPSG
                     WVTMQFVGAYAADNSTGLYLAVHDPWGSTKEIQLQSRPEDHIVTWAVEHPAENMGQAG
                     NHFELPGEAVFQAFAGDWFDMALIYRDWVRREARWYPKLGADGREDTALWMRELCLWA
                     LGGGAPADCVERVKRFAEYFGVPCGFHWYNWHQIPFDNDYPHYFPTKEGFAEAVAELQ
                     KSGVHVMPYINGRLWDTRDRGLEDFEFSSRAKPAVSKNEVGEPYREQYSSKESDGSPV
                     SLGVMCPSTSLWQETVQGIVARLFSECGVHGVYIDQIAAASPTLCFDASHGHPLGGGH
                     WWTTSYWQLLEGIRKTMPSERMITTECNAEPYIRWFDGYLSWHWQYPNQVPLFPAVYG
                     GTIQMFGRYYGGTGSGLRPTEPAGRNRDIALRMRASQQLVFGEQLGWINPAVVDEPQN
                     ASFLKKLVLLRSQAVRFFYAGEMVRPPRVAGNIPQLRADWQWQGSMWVTTDAVFTGAW
                     AIPRERKAIAFMVNAAEEELTVRVEADTRQWGLNASKIRTTCVLPEGRQQTEVGPSTW
                     AVEMTLPPCSAQAWEFEAIE"
BASE COUNT          998 a         1341 c         1148 g          908 t
ORIGIN      
        1 gggcggggcc tcttccatca aagaatcgta atatcgcgca atagtgctgg cggtactcat
       61 atcacctatt ttaacttggc tttggtgcta agtctgccgc gattttcgcc gcaatcaaac
      121 agattctgaa taggtccgcg ggctgccccg gctgcctacg ccggcggttc gacgtaacaa
      181 tcgaggcatt acacaccagc aggttacgcc gccagcctca cgatatatgt attgctgttt
      241 tcttgggtcc actttgtgca caaactgtgg taacgctgcc gtcacatcac ccgccgacca
      301 ctcacgttag ctgctggaaa ctgtaaatgt gagctagacc ccgcttttct atgtaagagc
      361 ctatccccaa aagcccccct ctccctccgg gaaatggttg gggtgaggcc aagcgaacgt
      421 cagaatcgat tgagaaaacc ggtagcgatt cgacccatgc tcacccccga ctcctctccc
      481 accaaacgcg tgaggggaga ttatgggata ggctctacat caaaacgtga tcagaactgg
      541 ggtttaccgt ctttcatggc ctggtaggcc tcgggcgtgt ccaccgagat accggggtct
      601 ttttgctcgg ccagccatcg gtcgagctcc tcagacaacc gctttttaat ctcagctact
      661 tccgggcgat cggcgagatt ggtcatttcg tatgagtctg ccgaggtgtc gtaaagctcc
      721 tcggcgggac ggacacagta ccgcttgacc aaccgataga cgtccggccg attccaagag
      781 tgggcgatcc aagtcggcca ataagcgttg tgggggacac tcccgcccag catgcccatg
      841 aggtgtttct cgatgtacat ctcctgtggt gtcaagtttc gaatataatg ccagcggccg
      901 tcactcacag aacggattgg atagggaggg ccttccggga agttattgtg cacgaaatag
      961 gcatatgttc ggtggctatc tttacggcca aggaggacct ccacaaagct tgtgccgtca
     1021 aagtcaccca tctctttggg ctggccccct gccagttcaa tcaatgtcgg gaccacgtcg
     1081 gcatattgga cgatggctgg ggtccgtttc cccgccggaa cacgccccgg ccagcgtgca
     1141 atcaaggccg tgtggacacc taggtcccag ttggtccact tgcagccagg gaactgcgcc
     1201 ccttgctccg atgtgaacaa aaccagtgtg tgctcgcgga gcccggcctc atcaaggact
     1261 cccaggattt cacccacctg cccatccatg taggtaattt cggccaagta ggaggcgaag
     1321 tcttcccgcg tcttcggggt gtcggcaata tggggtggaa gccgtagctt cttgacggga
     1381 taccgcgacc gatcccccat cacccagggc acgtgcggct caaccaaggc gaccaccagg
     1441 caaaatggct gatccttttt tcgggtcata aattcgcgga taccggccaa atcgtggggc
     1501 ggtgtgggat ccgccgtaca attgggacaa aaaccgggaa cattctcaaa aggaaatacc
     1561 tccttaggtg ttacgtgtac cttaccggca agtcccaccc gataacccaa acgaccaagg
     1621 taatggggta aactctggat accagggcgg gagccgcatg attccaggca caaccgttcc
     1681 gtatgggaaa caggcccgtg taaagctccg accgacacgg ttgacagatg gccatcgcca
     1741 gatacgctcg gtcaaatacg agcccttcct tcgccaggcg atcaatgttg ggtgtataag
     1801 cgttttcgcc cccgtaaacg ggcaagtcat tgtaggtgca gtcatctgcc atgatgataa
     1861 ggacatttgg acgatcggcc gccactgccg acaactcgac tacaccaacc gccaaaatac
     1921 ctagaagtaa cagagccgct cgcatagcta cctcccacat ccgcaattca gaatgaggca
     1981 ccaaaagcct gagactcata ataggctaac tagacggctc ccggaagtac atcgagccta
     2041 gcagcttgag tgaaaaaacc caacgccgcc accaatccat ttgcgtccaa accggttgaa
     2101 tcagataatt ctactcgatg gcttcaaact cccatgcctg ggcagagcaa gggggcaggg
     2161 tcatttccac agcccatgtg ctgggcccca cttccgtttg ctgccgacct tcgggaagca
     2221 cacaggtcgt gcgaatttta gaagcattca atccccactg gcgcgtatcg gcttccacgc
     2281 ggaccgtcaa ttcctcctcc gcggcattca ccatgaaggc gatcgccttt ctttcccgag
     2341 gaattgccca tgcacctgtg aacactgcgt ccgtggtcac ccacatagag ccttgccatt
     2401 gccaatcagc gcgaagctga ggaatgttcc cggcaacgcg aggtggccga accatctccc
     2461 cggcataaaa gaaccgcacc gcttgcgagc ggagcaaaac gagcttctta aggaacgagg
     2521 cattctgcgg ctcgtccacc accgcggggt taatccaacc gagctgttcc ccaaagacaa
     2581 gctgttgcga cgcccgcatg cggagggcaa tgtcgcgatt tcgtccggcg ggttccgtcg
     2641 gccgcaggcc actccccgtt ccaccgtagt atcgcccaaa catttggatg gtgcctccat
     2701 acacggcagg aaacagtgga acctggttcg gatattgcca gtgccaactg aggtagccgt
     2761 caaaccaacg gatgtacggc tcggcattac actcggtggt aatcatccgt tcggacggca
     2821 tcgtcttgcg aatgccctcg agcaattgcc agtaggaagt cgtccaccaa tgaccgcccc
     2881 ccaggggatg accgtggctt gcatcgaagc agagtgttgg cgaggccgcg gcaatttggt
     2941 caatatacac accgtggacg ccacactcgg agaacagtcg ggcgacaatt ccctggacgg
     3001 tctcttgcca aagggacgtg gaaggacaca tcacacccaa ggacaccgga ctgccatcgc
     3061 tctctttgct gctgtattgt tcccgataag gctcaccaac ttcatttttg gaaactgccg
     3121 gttttgcacg cgaagagaac tcaaaatctt cgagcccccg gtcacgtgta tcccagaggc
     3181 gaccattgat gtaaggcatc acgtgcaccc cgctcttttg aagttccgcc acggcctcgg
     3241 caaagccctc cttcgtggga aagtaatggg gatagtcatt atcaaatggg atttgatgcc
     3301 aattgtacca gtgaaaaccg cacggcacgc caaaatactc ggcgaatcgc ttcacgcgtt
     3361 ccacgcagtc cgccggggcc cctccaccaa gcgcccatag gcaaagttct cgcatccaaa
     3421 gggctgtgtc ttcccgacca tcggcaccca gtttggggta ccagcgtgct tcgcgccgca
     3481 cccagtcgcg gtaaatcaag gccatgtcaa accaatcacc ggcaaaagcc tgaaataccg
     3541 cctcgccggg taactcgaag tggttgcccg cttggcccat gttctccgcg gggtgctcca
     3601 ctgcccaggt aacaatatgg tcctccggcc ggctctggag ttgaatttcc tttgtgcttc
     3661 cccagggatc gtggaccgcc aggtaaaggc ctgtgctgtt atcagccgca taagccccca
     3721 cgaactgcat ggtcacccag ccactgggat agcgaccgcg aaactggagt gcccgctccc
     3781 acgccccctg ttcagcgatg cccacgcctt taggataaac cacccaggca tctcgccctg
     3841 gctcggcaat ggcaacctgg gggaacacca cggaccaaat gctccactcg gtgctctcgt
     3901 tttcgacttt tagccgccag cggatagcct ggcgcgattc ttcagcaatc gcctcgacct
     3961 cgacggcgat ccctttgagg cgcgcatcac tgaagtcctg ccagcggacc ttcaacgaag
     4021 aatgcccgtc ccctagttga caggtaacct tattccagcc agcgtcggcc gacaacacga
     4081 gctcttcctt gctgacagca tgtctcgccc gaatgcaaaa caagggcagg tcttctctga
     4141 cgaacaaccg atcctgacga acatcataag ccaccgggcc tttgagaccg ttcggaagtt
     4201 tttccataac cagcgaaaga ttgccggccg cgatgccggt caaaatctcg ggcatcgtgc
     4261 cttccgcagc gcgcagggca tcttcccaac gtccagttcg ctcgcaatag ctcgtcgccg
     4321 ccaagagaag aggcgaaagt tttgctcgcg ccgcgacaag acggttatga atctcacgag
     4381 ttgtcacgat ggcat
//
LOCUS       DAOPTR010000190         4394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16069_length_4394_cov_3.429362, whole genome shotgun sequence.
ACCESSION   DAOPTR010000190 DAOPTR010000000
VERSION     DAOPTR010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16069_length_4394_cov_3.429362"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2285)
                     /locus_tag="PKI05_04935"
     CDS             complement(<1..2285)
                     /locus_tag="PKI05_04935"
                     /inference="COORDINATES: protein motif:HMM:NF027656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4962 domain-containing protein"
                     /protein_id="HOA51578.1"
                     /translation="MKSASWLVINLLVICAVICGPQPTIGAETTLRLDPPVGPQQKPY
                     SPADDQVVEINPPPFIWVPAGKGVTYALQLSRSETFTEEPVRTYGNLHRSVFVPQEVL
                     PPGQWFWRYGIETEKGIVWGRARPFRIPEDARAFPFPTWDQIISKIPRDRPRLFFPGA
                     RLEEVRRLAAGPLKSQVNRLVDSCRRRVGEPLVPEPGYAPTGADRGPWAVNVMRTTRP
                     PMDVMESCALAWLLTGDHDLGQEAKRRILHFFGWDPNGPTSLFAYDEPAMWMMMRGTR
                     AYDWTYELFTPEERQRIESVMKIRAKQFLDRLQRMPFESNPYESHAGRLPGFLGECAL
                     SFIHEWPEAREWLDYATLLYYTSYPAWGGDDGGWQEGPAYWSAYMQFALHYVVALRAA
                     TGIDLMQKPFFRNTPYYALYTATPYHQHMPFGDGQTGNPRGLGHVMYVFSSLTQNPYF
                     RWYAEECGTTIGTDVLSLATYDPGLTARSPKELPQARAFPSVGLASIHTALGDRENDI
                     SFLLRSSPYGSVSHGHADQNAYVIEAFGRGLAIATGYYPWYGSPHHDRWTRSTRAVNS
                     ILVDGQGQVQRRWGMGGELVHFASTDGYDYVEAEAAAAYGGRLDRFRRHVVHIRPGVF
                     VMFDQLQTPNPSQFQWLLHAYNQIKIDETRQILNITNEPAALQVHLLLPEKLEFSQTD
                     RYDPEPEPIARDWQNTWHLTAATPAPAQRVHYLAVFLPHRVGQSATLPRVELIRGEGA
                     VGVRLKATDESEDLVGFRTDP"
     gene            complement(2799..3986)
                     /locus_tag="PKI05_04940"
     CDS             complement(2799..3986)
                     /locus_tag="PKI05_04940"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA51579.1"
                     /translation="MQTINVALIGQGLMGRSHSNAWSQVAKFFKPPIKPIMHTSFGQK
                     GVDPDPEGFAANWGWKNVSTDWESVVKNPEIGLVDIVTPNYMHAPVAKAAIAAGKHVA
                     CEKPIAGTLADAREMVQAAKKANVKTFVWFNYRRCPAVALAHLLVKQGKLGEIRHVRA
                     AYLQDWADENVPLVWRFDKNLAGSGAHGDLNAHIVDMTRFVTGMEITEIAGAIAETFI
                     KKRKLPSAGSTGAIVSGGAGKEEWGDVTVDDTVLFLARFSNGAVASFEAARQATGNQN
                     RNMFEINGTKGALKFDFERMNELLFYDATLPRAVQGWTNIMVTHGADHPYVANWWPDA
                     HIIGYEHGFVNQAYDILKVIAGEEPTVPIPDFEDAYQTQRVLEAALISATERRPVKLD
                     EVY"
BASE COUNT          937 a         1272 c         1233 g          952 t
ORIGIN      
        1 tgtggatcgg tacgaaagcc tacgaggtcc tcgctttcat cggtggcctt gagccgtact
       61 ccaacggcgc cttcaccgcg aatcagctcc acccgtggta gggtagcact ttgaccgacg
      121 cgatggggca aaaacacagc aaggtaatgg acccgctggg caggggccgg tgttgcagcc
      181 gtcaagtgcc atgtgttttg ccaatcgcgg gcaatcggct ccggctccgg gtcataacga
      241 tccgtttgag aaaactccag cttttccggc aagaggaggt ggacctgaag ggcggcaggt
      301 tcattcgtga tattcaagat ttgcctggtt tcatcgatct tgatctgatt gtacgcatgc
      361 aagagccatt gaaactgcga agggttaggt gtttgcaact ggtcgaacat gacaaagaca
      421 cctgggcgga tatgcaccac atgccgccgg aaacgatcca gacggccacc gtaagccgcg
      481 gctgcctctg cctcaacgta gtcatagccg tccgttgagg caaaatggac caactctccg
      541 cccattcccc atcgtcgctg gacctgccct tgtccatcca cgaggatact gttcaccgcc
      601 ctggtcgatc gggtccagcg atcgtgatga ggtgatccat accagggata gtatcccgtc
      661 gcaatggcca agcctcgccc aaaagcttcg atgacatagg cgttttggtc ggcatgccca
      721 tggctgacac taccataggg gctgcttcgc aaaagaaaac tgatatcatt ctcccggtcg
      781 ccgagggccg tatgaatgga ggccaaaccg acgctcggaa aagcacgtgc ctgtggtaac
      841 tctttgggtg accgggctgt taagccgggg tcgtaggtgg ccaggcttaa cacatccgtg
      901 ccaatcgtcg tgccgcattc ctccgcatac caacggaaat agggattctg cgtgagagag
      961 gaaaaaacgt acatgacgtg ccctaaaccc cgcgggtttc cggtctgacc atcgccaaag
     1021 ggcatgtgtt gatggtaggg cgtggccgtg tagagggcgt aatacggtgt gtttcggaag
     1081 aagggttttt gcattaggtc gatcccggtg gctgcccgca gtgccaccac ataatgcaac
     1141 gcaaactgca tgtaagcgct ccagtatgcg ggaccttctt gccaaccgcc atcatcgcca
     1201 ccccatgccg gataggaggt gtaatacagc aaggtggcat agtcgagcca ctctcgggct
     1261 tcaggccatt cgtgaataaa agaaagcgca cattcgccaa ggaagcccgg caaacgtccg
     1321 gcgtggcttt catacgggtt gctttcaaac ggcatccgct gaaggcggtc caggaattgc
     1381 ttggctcgga tcttcatgac ggattcgatc cgctgccgct cctccggcgt gaacagctcg
     1441 tacgtccagt cgtaagcccg cgttccccgc atcatcatcc acatggccgg ttcgtcatag
     1501 gcgaacaggc tcgttggacc gttgggatcc caaccgaaaa agtgcaggat gcgacgtttt
     1561 gcttcttgac ccagatcgtg gtcacctgtc aacagccagg ccaacgcaca agattccatc
     1621 acatccatcg gcggccgtgt ggtccgcatc acattgacgg cccagggccc gcgatccgcg
     1681 cccgtcgggg cgtagcccgg ttcgggcacc aatggctcgc cgacacgccg ccgacatgaa
     1741 tcaacaaggc gattgacctg ggatttcagc ggtcccgctg ccaggcgtcg gacttcctcc
     1801 aaacgtgccc cggggaagaa cagacgcggt cggtctcgtg gaatctttga aatgatctga
     1861 tcccaggtgg ggaatgggaa ggccctggcg tcttccggga tacgaaatgg ccgcgctcga
     1921 ccccaaacga tgcccttttc tgtctcgatc ccatatcgcc aaaaccactg tccgggcggt
     1981 aaaacttcct gcgggacaaa gacggatcgg tgaaggttgc cgtatgtgcg gacgggttcc
     2041 tccgtgaacg tttcggaacg ggagagttgc aaggcgtagg tgaccccctt tcccgcgggg
     2101 acccaaataa agggcggggg attgatttcg acaacttgat catctgctgg tgaatagggt
     2161 ttttgctggg gtcccactgg aggatccagc cgcagtgtcg tttcggctcc aattgtaggc
     2221 tgtggtccac aaatgactgc gcagatgacc aacaaattga taacaagcca agatgcgctt
     2281 ttcatggaag gtctcctcgg ttggctaagg ctttctaacc cgacacgtcc accctgaggt
     2341 aatctttcgg caggaaccgg ttgcttgctg atcaccatcg ttaagggtcg agaggtggct
     2401 gtcaacaggg cgttcaagcc gcagggatgt gcacgtccgc tcgtggctct gggaaggatc
     2461 gtagatcatc cattgacact aagagcctac cctgtaatct cccctctccc gttcagcggg
     2521 agagggattg ggggtggggg tgacttgaat cgctgccggt tttctcagta gattcctacg
     2581 atcgcttgcc ttcagcccaa ccctctccca gagggagagg gggagttttt ggtatccgct
     2641 ttaaggcagg cgcgacggcg cttccctcca gatcaggcgc aaaaaagcgg cgccttggat
     2701 gctcgctctc cgcggtccaa ggcgccggaa aatgcgactt cctgtcggca cagaatttca
     2761 caaacgacag gtgtcttccg cggtcctcgc cgtttcgatc aatagacctc gtccagtttc
     2821 acaggtcgcc gttcggttgc cgagatcaac gccgcttcca ggacccgctg tgtctgataa
     2881 gcatcctcaa agtccggaat gggcacggtc ggctcttcgc ccgcaatgac cttcaaaatg
     2941 tcgtacgcct gattcacgaa accgtgttcg tagccgatga tatgggcatc cggccaccaa
     3001 ttcgcgacgt agggatgatc ggccccgtgg gtgaccatga tgttcgtcca accctgcacg
     3061 gcacgaggca gcgtggcatc atagaataac agctcgttca tccgttcaaa atcgaactta
     3121 agagcaccct tcgtgccgtt gatttcgaac atgttgcgat tttggttacc cgtcgcttga
     3181 cgtgcggcct caaagctggc cacggcgccg ttggagaagc gggcaaggaa tagcaccgtg
     3241 tcatcgactg tgacatcacc ccactcctct tttccagcgc ctccggagac aatggccccc
     3301 gtcgaaccgg cactggggag cttgcgcttc ttgatgaagg tttcagcaat ggcgccggca
     3361 atttcggtga tctccatgcc cgtgacaaag cgggtcatat cgacgatatg ggcattgagg
     3421 tcaccgtggg ccccagaccc ggcgagattc ttatcgaacc gccacacaag aggcacgttc
     3481 tcgtcggccc aatcttggag ataggcggcc cgtacgtgac gaatttcgcc gagcttgccc
     3541 tgcttgacca aaaggtgggc taacgcgacc gccgggcagc gacgataatt gaaccagacg
     3601 aatgtcttca cgttggcctt cttggcggcc tggaccatct cgcgggcatc ggcgagcgtc
     3661 cccgcaatcg gcttttcgca ggccacgtgc ttgcccgctg caatcgctgc cttggccact
     3721 ggggcgtgca tgtagttggg tgtgacgata tcgaccaagc cgatctcagg gttcttcacc
     3781 actgactccc aatccgtgga aacgttcttc caaccccaat ttgcggcgaa gccctctgga
     3841 tcggggtcca cgcccttctg gccaaaggac gtgtgcatga tcggcttgat cggcggcttg
     3901 aaaaacttgg ctacctggct ccaggcattg gaatgggacc gacccattaa cccctgaccg
     3961 atcagcgcga cattgatcgt ttgcatggca agctcctccg aatgttgata actcttgcga
     4021 ccttgtttga taagcccttt ggaccgccgt tatatcgcat tttgctacgg attcaagtgg
     4081 cctcgaaaaa acacctttgt tcgccgccgg aaaatcttcg ctatcttgac caacctgtta
     4141 tcagaccgct gggttgccgc atacccgccc ccacggcata ccacctatcc gatgagggac
     4201 cgtaaaatcc atatattaac gcttgccgat tgcgaaggaa atagcccgag ccaggcgttt
     4261 ttgcgatagc accttccact tcccgcagac tttgtatgaa ccacgaagac tttgatgttc
     4321 acggacttgc aacatacctt cacctgacgc cgcaacaggt gcaacggctc gccgatcggg
     4381 ggaagattcc cgga
//
LOCUS       DAOPTR010000191         4386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16111_length_4386_cov_3.165782, whole genome shotgun sequence.
ACCESSION   DAOPTR010000191 DAOPTR010000000
VERSION     DAOPTR010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16111_length_4386_cov_3.165782"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PKI05_04945"
     CDS             complement(<1..721)
                     /locus_tag="PKI05_04945"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOA51580.1"
                     /translation="MRPTQLWLTASLSKSLPQTRGLLCPDNGTKAAMIRFLIDGYNFL
                     NASGIDTQRFDFSGTTPLERSRLALLDFLVTHLTPEEISETVVVFDARHFLRPQADEF
                     TYHGLTVRFAARYPDADSLLEEMIQSHSAPKQLTVISSDHRIQRAARRRRAKFIDSES
                     WYRHLCARHHRQGTTIQSKAGTEDKEVVNLSPHELKHWLEEFASSCKLSEETPHSVSE
                     TRRPESNDREHPTSSNSGDEAV"
     gene            complement(718..1998)
                     /locus_tag="PKI05_04950"
     CDS             complement(718..1998)
                     /locus_tag="PKI05_04950"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOA51581.1"
                     /translation="MERLLIRGGRVIDPSQGLDREMDILIQGGQIVEYDPPVRGDEVV
                     IDAKGKIVAPGLIDMHVHLREPGQEDDETIATGTAAALAGGFTSIVCCPNTDPPIDSP
                     ATVEFVRQKAARADNCHVFVMACVSKDRKGQELAEIGQLVAAGAVAFTDDGAPVYDPE
                     LMRRAFEYCLMFDKPVCNHCEIPELARNGVMHEGLVSLILGLPGIPAAAESVMVARDI
                     ALAEATGGRLHVMHVSTLDSVEAIRRAKKRGVRVTAEVTPHHFTLTDECLRTFDSNYK
                     MNPPLRSRDHVEACINGLRDGTIDVIASDHAPHAKEKKMQELDRAPFGIIGLETSLSL
                     VITKLIKPGLLTWSEALAKMTINPARILGLNKGTLAIGADADVTIIDPNYQWVVDPSA
                     FLSKARNTPFAGWKLEGRATEVIVSGTIKYRIGP"
     gene            complement(2062..3024)
                     /locus_tag="PKI05_04955"
     CDS             complement(2062..3024)
                     /locus_tag="PKI05_04955"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOA51582.1"
                     /translation="MDDIESKLAPFRGRWNRSHLLSLEELSREELLCILDAADVFRDA
                     FDRGLRKWPVLTGKTCVNLFFENSTRTRNSFSLAARRLGADVVDFAASTSSLSKGETV
                     VDTARNLEAMGIEAVIVRHSTPGTPHLLANHLRCSVINAGDGAHEHPTQGLLDIMTIR
                     RRLGRIEGLTVALIGDIAHSRTARSNIWGLTKLGAHVIVCGPSTLVSPLWRELGVEYS
                     YNLDDILPRVDVLNLLRIQFERQTRRPFPSVREYASLYAMNAARLSRAKPDILIIAPG
                     PINRGVEITADVADGQHSAILDQVTNGVAVRMAVLWLLITRQKE"
     gene            3240..4274
                     /locus_tag="PKI05_04960"
     CDS             3240..4274
                     /locus_tag="PKI05_04960"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOA51583.1"
                     /translation="MSTRPRFIVYVIGSQGMVQRIEAALRPVKLISEVIAVRDDALHS
                     QGDSTALTEDADTNGTSYEATLALLVTDTTWDKLKLLALCRRYMPYRHDPRLTAVLRV
                     GTREGQGTHISAGCHAADLRAIVSLLRRNLELRHYLLEKQSLVKALHSMAVRDPLTGL
                     INRRGWKIGIKKLWQEAAGRDGFLAVALFDLDGFKKINDQFGHAFGDHVLKTVAAQAQ
                     KACRKGDLLARWGGDEIALAVVLPDKAVARGLVERVQNSLRSDFPQIGQLVTACAGYV
                     VVKVVTTWRPDFGKRLLEAADAVLTQAKASGTGRVCEGNVVLSDEEKSIADKSPGGAG
                     DSSTDEHSES"
BASE COUNT         1013 a         1203 c         1209 g          961 t
ORIGIN      
        1 gaacagcttc gtccccggag ttgctgcttg tagggtgttc ccgatcgttg gattctggac
       61 gccgtgtttc actgacagaa tgaggtgttt cctcggaaag cttgcacgac gaagcaaact
      121 cttctaacca gtgctttaac tcatgagggg aaagattcac cacctcttta tcctcggttc
      181 ccgcctttga ctggatagtc gttccttgac gatgatgtct tgcacacaag tgacggtacc
      241 aagattcgct gtcaataaac ttggccctac gtcggcgtgc cgccctttgg atacgatgat
      301 cacttgaaat gacggtcaat tgtttcgggg cagaatgaga ttggatcatc tcttccagaa
      361 gtgaatccgc gtcggggtag cgggcggcaa agcgaacggt caagccgtga taagtaaact
      421 cgtccgcttg aggccgaaga aaatgccggg catcgaagac cacgacagtt tctgagattt
      481 cctcgggcgt aagatgggtg acaagaaaat ccaataacgc caagcgacta cgttccaaag
      541 gggtagtccc ggaaaagtcg aagcgctggg tatcgattcc ggatgcatta aggaaattgt
      601 aaccatcaat caaaaagcga atcatcgccg cttttgtgcc attgtcgggg caaagcaaac
      661 cccgggtctg cgggaggctc ttgctaaggg acgcagttag ccacagttgg gttggcctca
      721 agggccaatc ctgtacttaa ttgtacccga aacaatgacc tcggttgccc gaccttcgag
      781 tttccatccc gcgaagggag tgttgcgggc ctttgagaga aaagcactcg gatcaaccac
      841 ccattggtag ttaggatcaa taatggtcac gtcggcatcc gccccgatgg ccagcgttcc
      901 tttgttgaga ccgagaatcc tggcgggatt gatcgtcatc ttggccaagg cctccgacca
      961 cgttaaaagc cccggtttga tcagtttggt gatgaccaga ctcaggcttg tttcgagccc
     1021 aatgatcccg aagggggctc ggtccaattc ctgcatcttc ttttctttag catggggtgc
     1081 gtgatccgag gcaataacat cgatcgttcc atcgcgcaaa ccgttgatgc aggcctccac
     1141 atggtcccgg gaccgtaacg ggggattcat cttatagttt gaatcaaaag tcctcaaaca
     1201 ttcgtcagtt agcgtgaagt gatgcggggt tacctccgcg gtgacccgaa ctccgcgttt
     1261 tttggcacga cgaattgcct ccacgctatc caaggtggac acgtgcatga cgtggagtcg
     1321 gcccccggtg gcctctgcta aggcaatatc tcgggcaacc atgacggact ccgctgccgc
     1381 cggaatccca gggagaccaa ggatcaggga cacgaggccc tcgtgcatga ctccgtttcg
     1441 ggccaactct gggatttcgc aatgattgca gacgggctta tcgaacatca ggcaatactc
     1501 aaaggcccgt cgcatgagtt caggatcata gacaggcgca ccatcatccg taaacgcgac
     1561 agccccggcg gcaaccaatt ggccaatctc ggccaactct tgccccttgc ggtccttgct
     1621 tacacaagcc atgacaaaga catggcagtt atcggcccgt gctgcctttt gccgaacaaa
     1681 ctccacggtt gctggtgagt caattggtgg atcggtgttt gggcagcaga cgatggacgt
     1741 aaaaccgccg gccagggccg cggctgtgcc cgtggcgatt gtttcatcgt cctcctgtcc
     1801 cggttcccgg agatggacat gcatgtcgat gagcccgggc gcaacaatct tgcctttggc
     1861 atcgatgacg acttcgtcgc cacggacagg cggatcgtat tcaacgattt gcccgccctg
     1921 aatgaggatg tccatttccc gatccagacc ctggcttggg tcgatgaccc ggccaccacg
     1981 aattaggagt ctctccatgg tcgttcacct ggcagatatc tgcatcgttt cttcgttctc
     2041 tcagaattgg tcgagtccaa gctattcttt ctgtcgcgtt atgagaagcc acagaacggc
     2101 cattcgtaca gccacaccat tggttacctg gtcgagaatg gccgagtgct gaccatcggc
     2161 gacatcggcc gtgatttcca caccccgatt gatgggacct ggggcaatga tcaagatgtc
     2221 gggcttggcc cgcgataacc gcgcggcgtt catggcgtac aaactcgcgt attcgcgcac
     2281 cgaggggaag gggcgacgag tttgtcgctc gaattgaatc cggaggagat tcaagacgtc
     2341 tacccggggg agaatatcat ccaaattgta ggagtactcg acccccaatt cccgccatag
     2401 aggagataca agcgtggacg gaccgcaaac aatcacatgg gcccccaatt tggtgagtcc
     2461 ccagatgttc gaccgtgcgg ttcgactatg agcaatgtct ccaatcaggg ccacggtgag
     2521 gccttcgata cgtcccaagc ggcgccggat cgtcatgatg tcgagcaacc cctgggtagg
     2581 atgctcgtgg gcaccgtcac cagcattgat caccgaacat cgcaagtgat tggcgaggag
     2641 atgcggtgtc cccggcgtgc tatgtcgaac aatgaccgcc tcaatgccca tggcttccag
     2701 attgcgggcg gtatccacaa cggtctcgcc cttggatagg ctgctggtcg aggcggcgaa
     2761 gtccacaaca tcggccccca gccgccgagc agctaaggaa aaactattgc gggtccgtgt
     2821 ggaattctcg aagaaaagat tgacgcacgt cttgcccgtc agaacgggcc attttctcag
     2881 cccccgatcg aaggcgtcac gaaacacatc ggccgcgtca agaatacaaa ggagttcctc
     2941 ccgggacaac tcctcaagac ttaagagatg agacctattc cagcgccccc ggaatggcgc
     3001 taattttgac tcgatgtcat ccatacaaag aactcgcacc aaaaaggctg aacgttcctc
     3061 aggtcaccag cgatacccat catgtcggaa gaccatagat accatcgggc ccaattggcg
     3121 taaaatcaac ctagccgacg attctacgac ttaatactca gtactgcaag gaccacgatt
     3181 tcggacgtgt ttggaagatc cgtaaatccc cgctgaatga tcctcttaga ggacacgtca
     3241 tgtccacacg acctcggttt atcgtttacg tgatcgggtc ccaaggcatg gtgcagcgaa
     3301 tagaagcggc actccgtcct gtgaaactga tttcggaagt catcgccgtt agggacgacg
     3361 ctttgcacag tcaaggcgac agcaccgctc ttaccgaaga cgcggacacg aacggaacct
     3421 cgtatgaggc aactttggcc ctcctggtta ccgacaccac atgggacaag cttaaactct
     3481 tggcactttg cagacgctat atgccatatc gccacgatcc tcggttaacc gctgtgctgc
     3541 gggttggcac gagagaaggg caaggaactc acatttcggc ggggtgtcat gccgcggacc
     3601 ttcgcgccat cgtgtcgctg ttacggcgaa acctggaatt gcgccactat ttactggaaa
     3661 agcaatccct cgttaaagcc ctacactcga tggccgttcg tgatccccta acggggttga
     3721 tcaaccgtcg cggctggaag atcggcatca agaagttatg gcaggaagcc gcaggacgtg
     3781 atggcttttt ggcagttgcc ctgtttgatt tggacggttt caaaaaaatc aatgaccagt
     3841 ttgggcatgc ctttggcgac catgtcctaa agactgtggc ggcacaggcc cagaaagcct
     3901 gtcgaaaggg ggaccttttg gcccgttggg gcggtgacga aatcgctttg gccgttgttt
     3961 tacccgacaa agctgtcgct cggggccttg tcgaacgggt acaaaacagc ctgcgatcgg
     4021 attttccaca gatagggcag cttgtcactg cgtgtgcagg ctacgtggtc gtgaaggttg
     4081 tcacaacctg gcggccagac ttcgggaaac gccttctgga ggctgcagat gcggtcctca
     4141 cccaggccaa agcctctggg acaggtcgcg tttgcgaggg aaacgttgtt ctttccgatg
     4201 aagagaagtc gatcgccgac aaatcccccg gtggggcagg ggacagcagc acggacgagc
     4261 acagcgagtc ttagccttga cgattttgtg tcaatcgcac aaagaactct tcgagcgtcg
     4321 gttctagcgg gtgaaatgca cttacctgca tgccacgacg aaccaattcc tgaaggacct
     4381 gcgcca
//
LOCUS       DAOPTR010000192         4382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16130_length_4382_cov_4.672290, whole genome shotgun sequence.
ACCESSION   DAOPTR010000192 DAOPTR010000000
VERSION     DAOPTR010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16130_length_4382_cov_4.672290"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PKI05_04965"
     CDS             complement(<1..231)
                     /locus_tag="PKI05_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOA51584.1"
                     /translation="MTSDNPEIGRHLATGDIVTNYHDLGSGAPLLLLHGSGPGVSAYA
                     NWRRVLPALAEHARVLAPDLAGFGYSEIPAGLA"
     gene            442..1059
                     /locus_tag="PKI05_04970"
     CDS             442..1059
                     /locus_tag="PKI05_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOA51585.1"
                     /translation="MQRSRRFRRVVATFVALVVSISGVACSGPTRTASHPPPGTIPPQ
                     AGGQRKPTDVAWDGVIPSSLLFETNSYAIRDGSHADFRKVITELNTQSPNHDALVGII
                     RGYADGVGEAEYNQTLSLRRAESVATLLTKMFQDDNWPNVTLAAVGCGQRGDVVGNTH
                     TASPVLATPGRDDPARRTVSITIYGKAKIDAEVGLEAQAKLCEKS"
     gene            1044..1802
                     /locus_tag="PKI05_04975"
     CDS             1044..1802
                     /locus_tag="PKI05_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51586.1"
                     /translation="MRKIVAIIVAMMLAVPLVGCDDGDWNDCQSWQSSTEQGETQTVY
                     ILIQPSSNSEELATHLRNDLKPFLESLVEKGGAPLRLEGWAYGGLGTPIRQLKCMNGA
                     VYPLKSPNSQIQRELREVIPGTVVKAASEELMDTQIGKTRADALPLLLKISKPEKAHV
                     FLWSQFNANGEYCLQETLPAPIVEQAKKKIQGCVDAGDIHVLPGLASFTINGLGVMTD
                     EKSRNMQPYSLGLAKYLCEAVTGSPGVCHTPPVK"
     gene            1813..2784
                     /locus_tag="PKI05_04980"
     CDS             1813..2784
                     /locus_tag="PKI05_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51587.1"
                     /translation="MAVAELGSIANKIFRIGDPTTEAGRDYQLGMLMAARDMEIANAL
                     TSIETSLVRLEAGRVIGDAETFKRVGDGVAALDAARTRDVNAWAEAGLANALPVWHVP
                     GWLRALLTLLMAGLDFYIFANAISKALRASDDPGLPQFWLGGATGLLVFIVGVAMAHN
                     IKNRSWLKVLAENDPQERNALWRESLSAGKTALVVMVTVYGLLTVVALGVRFGQVQKP
                     ISIWVVDPGARGEIAAIILFTLVPLVAVALEVALHDPLAPPPPMPVRIGLIGRIRASV
                     RAKRHAVLMLRKEKVSEAIQKRYAFELDALFALQQQHIQKGATDGAQ"
     gene            2786..4228
                     /locus_tag="PKI05_04985"
     CDS             2786..4228
                     /locus_tag="PKI05_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51588.1"
                     /translation="MRKKRSAWGRFWAVTRLEGVSRSDKRKILSILNEYVRQLGDGEA
                     DRPEELSYLERGLIAQSASSYAQLEEARARIDEGVRQLVQNQQAFAAREAELAAAKEV
                     PIDDLPTPELDVAEASVNEVKSRVGDLLRLRESLSGRQALGRMMNPRLRDAITIVLFV
                     LQFLLYLKPIVDAIIGGQYSSVWTAIADGLLGAAISASAPLVVLTTSVALGRVLSAKR
                     IEDDIQRLGLVERVLPLNVPSAADGAMQKVVEWLQGRVQTSWAGLSLIAAMALVMMSL
                     LLGFATRFGLNFLVAAPEGGLEGPLSLVSVLLVIFPIGVLVAHVAGSNELADAVAAAE
                     RELDEAETRLRAEVKQAATERAMKKAALVGAHRALLYAGEQRRAAAGTLEKLVSDLDA
                     IAPLVEHQLIGGAVRLISIERGVNDRLPLNYVEEVDGKAIAEGIASVLSSIGEEISAL
                     KRQGQTALDAWPVPKAPTDPAQGAEPGAGS"
BASE COUNT          856 a         1207 c         1400 g          919 t
ORIGIN      
        1 ggccagcccg gccgggatct cggagtagcc gaagcccgcc aggtcggggg ccagcacgcg
       61 ggcgtgctcg gcgagcgccg gtaagacccg gcgccagttc gcgtaggcgc tcacccctgg
      121 ccccgagccg tggaggagga gcagcggcgc gccggagccg aggtcgtggt agttcgtgac
      181 gatgtcgccg gtcgcgaggt ggcgtccgat ctctgggttg tcgctcgtca tgattcctcc
      241 aatggcgcgg gtggacgccg tccgtgcggg ctcggcggac cgctgaggtc gcccacgacc
      301 ccgctctata gccaccttta gcacgtgggt gcacatcagt ggaagagtca atcgcgtcgg
      361 tcggcccact tcatgaaatc ccttccagaa cttccaccgc gccctaggct gtgcgtgccg
      421 catgagaggc aggggggatt catgcaacga tcacgtcgtt ttaggcgggt ggtggcgacc
      481 ttcgtcgccc tcgtagtgtc catctccgga gttgcatgct ctgggccgac acgcactgca
      541 agccacccac cgccgggaac tattcccccg caggccgggg gccagcggaa acccacggac
      601 gtagcttggg acggtgtgat cccgtcctca ctgctctttg aaaccaactc gtacgccatc
      661 cgtgatggct cacatgccga cttccgcaag gtcataacgg aactgaatac ccaaagtccg
      721 aaccacgatg cactggtcgg tattattcgc ggctacgcgg acggagtggg cgaggccgaa
      781 tataatcaaa cgctgagcct taggcgcgcc gaatcagtcg ccacgctctt gacgaaaatg
      841 ttccaagatg ataattggcc caacgtcacc ctggctgccg ttggctgcgg acagcgcggc
      901 gacgtcgtgg gcaacaccca caccgcaagc ccggtgctag caacgcctgg ccgagacgac
      961 cctgcgcggc gaactgtatc aatcaccatc tacggaaaag cgaaaatcga cgctgaagtc
     1021 ggtcttgagg cacaggctaa attatgcgaa aaatcgtagc aatcattgtg gcgatgatgc
     1081 tcgccgttcc actcgtgggc tgcgacgacg gcgactggaa cgactgtcag tcctggcaaa
     1141 gtagcaccga gcaaggcgag actcagaccg tctacatcct catccagccc tcaagcaact
     1201 cggaggaatt ggcgactcac cttcgcaatg atctcaaacc attcttggaa tcgcttgtag
     1261 agaagggggg tgcgcccttg aggcttgagg gatgggctta cggaggtctg ggaactccaa
     1321 ttcggcagct gaagtgcatg aacggcgccg tgtatcctct caagagtccg aattcacaga
     1381 ttcaacgcga gttgcgtgag gtgatccccg gcactgtcgt caaagctgcg agcgaggagt
     1441 tgatggatac ccagatcggc aagactcggg ccgatgctct tccgctcctc ctgaagattt
     1501 ccaaaccaga gaaggcgcac gtgttccttt ggagtcaatt caatgctaac ggcgaatatt
     1561 gcctgcaaga gacgctgcct gctcccattg tcgagcaagc aaagaagaag attcaaggat
     1621 gtgtcgatgc gggtgatatt catgttttgc caggattggc gagctttact atcaatgggc
     1681 tcggtgttat gaccgacgaa aagtcaagaa acatgcaacc ctattctctg gggcttgcca
     1741 aatatctgtg tgaggcagtg accggaagcc cgggtgtctg tcatacgccc ccggtcaaat
     1801 agggtcgacg ccatggctgt agctgagctg ggttcaattg caaacaagat ctttagaatc
     1861 ggcgatccca caacggaggc gggccgtgac tatcagttgg gcatgctcat ggcagccagg
     1921 gacatggaga tcgccaatgc gctgacgagc attgagacgt cactggtacg cctcgaggcc
     1981 gggcgcgtca tcggagatgc cgaaacgttc aagcgggtcg gcgacggcgt cgcggccctc
     2041 gatgcggcca ggaccagaga tgtgaacgcc tgggcagaag ccgggcttgc caacgcactt
     2101 cccgtgtggc atgtcccagg atggctccga gccttgttga ccctgctcat ggcgggactt
     2161 gacttctaca tttttgccaa cgcgatcagt aaggcattgc gcgccagtga cgatcctggc
     2221 ctcccgcagt tctggctcgg tggggccacg ggtcttctcg tctttattgt tggtgtcgcc
     2281 atggcgcaca acatcaagaa tcgttcatgg ctgaaagttc tggcagagaa cgatccccaa
     2341 gagaggaatg ctctctggcg cgagtcgctg tcggcgggga agacggcctt ggtggtgatg
     2401 gttactgttt atggtctcct aacggtggtg gctcttgggg ttcggtttgg gcaagtccag
     2461 aaaccgatca gcatttgggt tgtggatccg ggcgcgcgcg gggagattgc ggccatcatt
     2521 ctcttcacgc tggtcccact cgtcgccgtt gcgcttgagg tagcccttca tgatcctctt
     2581 gccccgcctc ctcccatgcc ggtcagaatc gggctgatcg gtaggattcg ggcttccgtg
     2641 cgggccaagc ggcatgccgt cttgatgttg cgaaaggaga aggtatcgga agctattcag
     2701 aagcgttacg cgtttgaatt ggatgcactc tttgccttgc agcagcaaca tattcagaag
     2761 ggtgccactg atggggccca gtaaaatgcg gaagaagcgt tcggcgtggg ggcgattctg
     2821 ggccgtgacc cgccttgagg gcgtttcgcg ttcggataag agaaagattc tttccatcct
     2881 caacgagtat gttcgtcagc tcggcgacgg agaggcggat cggccggagg agctgagtta
     2941 tctcgagagg ggtctcatcg ctcagtcggc cagttcttac gcccagttgg aggaagctcg
     3001 tgctcgaatc gatgaagggg tccggcagct cgtgcaaaat caacaagcat tcgctgctcg
     3061 tgaggcggag cttgctgctg cgaaagaggt cccgatcgat gatctaccga ccccggagtt
     3121 ggatgtggcg gaagcaagcg tcaacgaggt caagtcgagg gttggtgatc tgcttcgact
     3181 cagagaatcg ttgagtggtc gccaggcgct tggccgcatg atgaatcctc gcctcaggga
     3241 tgcaattact atcgttcttt ttgttctgca atttctcttg tacctaaagc cgatcgtgga
     3301 cgcgatcatc ggagggcagt actcgagcgt ctggactgct atagcggatg gtctcttggg
     3361 ggctgcgatc agcgcgtctg cacccctggt ggttctcacg acgtcggtgg ccctcggtcg
     3421 cgtgttgtcg gcgaagcgga ttgaagacga cattcaacga cttggtctgg tcgagcgagt
     3481 tcttcctctg aacgtgccaa gtgctgcgga tggcgccatg cagaaggtgg tggaatggct
     3541 gcaagggagg gttcaaacta gctgggccgg actgtcgttg atcgcagcta tggccttggt
     3601 catgatgtcg ctgctgcttg gctttgcgac tcgcttcggg ctcaactttc tggttgccgc
     3661 gcctgaaggt ggacttgaag ggcccttgag cctcgtgtcc gtgctcctag tcatcttccc
     3721 gatcggcgtg ttggttgcgc acgtggctgg ttcaaacgag ttggccgatg cggtggccgc
     3781 ggccgagcga gagctggatg aggctgaaac acgtctgcgc gcggaagtga agcaagccgc
     3841 tactgagcgg gcgatgaaaa aagccgcttt ggtcggcgcc cacagggccc ttctctatgc
     3901 tggggagcag aggcgcgccg cggctggcac cctagagaag ctggtgtcgg acctcgacgc
     3961 tattgcaccg ctcgtggagc accaattgat cggtggcgct gttcgcctta tttcgattga
     4021 gcggggcgtt aacgatcgac tcccgttgaa ctacgttgaa gaggtcgacg gcaaggccat
     4081 cgcggagggg atcgcttcgg tcttgagttc catcggtgag gagatctcgg cgctgaaacg
     4141 ccaagggcag actgcacttg acgcgtggcc agtacctaag gcgcccactg atcccgcgca
     4201 gggtgctgag ccaggcgccg gttcttgaga ttcatgtgac gaagatcatg cgcaggtctc
     4261 cggtggcgat gcgctgccgg aagcacgcgg gtggcaccca gcctctcggg gtggatcccc
     4321 ggccaacggt cgacgtgcgg gtctgtcacc tgagcctcga tccgccgaag cgcattcgta
     4381 gg
//
LOCUS       DAOPTR010000193         4372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16174_length_4372_cov_4.044475, whole genome shotgun sequence.
ACCESSION   DAOPTR010000193 DAOPTR010000000
VERSION     DAOPTR010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16174_length_4372_cov_4.044475"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="PKI05_04990"
     CDS             complement(<1..1167)
                     /locus_tag="PKI05_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOA51589.1"
                     /translation="MAFEELPPLDAMAERLQRDVRRGSSAVFVAQVVSQLISLVVLAI
                     LLRTLGLEPYGLLGMVLPLLALVRIFIYSGLDVAAIQDPLLDRQRASALFWINQILGM
                     LGTAIVAVSAPWISWFYGREELVPLTIALSGTMLVTTLGAQHQALLQRDLQLGRLSII
                     RVAAQVIGGVAALIAAWAGAGIWTLVVQQYAEPAALTVLSWVVTGWRPAFVLRGAGSR
                     ELLHFGGHYTMSAFLLYLMSHVDKIVVGRFLGSQALALYYQAFNLAQKPVGLVVSPLT
                     GVMLPALARARSEASLYRQYATRFLRFLNWIMFPCGIGLALVADETMAVLGGPRWSGA
                     GPILRIFALMIPFQALFNVTGTIYASLGRADRMARACIPVTAVMCVAFLVTVTVA"
     gene            complement(1187..2053)
                     /locus_tag="PKI05_04995"
     CDS             complement(1187..2053)
                     /locus_tag="PKI05_04995"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOA51590.1"
                     /translation="MNSPSLDPERLRRGLVQYLEDMQHAGINHLIWPSAETIDSSLLG
                     SQQVSSDDTRLGQSEPLEAQARQGSEENRVTGQLPVVDTVAPLGEIQPLSESREDVLA
                     ELAKQVAQCTRCPELVANRTQTVFGVGNPYSELVFIGEAPGVDEDKQGEPFVGRAGQL
                     LTAMITNGMGMRREDVYICNILRCRPPNNRTPTREEADRCRPWLDATLAVIQPKYICC
                     LGNVAAQNLLRTDVGIGKLRGKVWEYDRAKVVCTYHPAFLLRNPAMKREAWEDLKLLL
                     QVMGRPVPRRPS"
     gene            complement(2213..2677)
                     /locus_tag="PKI05_05000"
     CDS             complement(2213..2677)
                     /locus_tag="PKI05_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51591.1"
                     /translation="MPNEQVVKVVWPSITAWPLGRWVGRVWGWALARGPLAALCVGFF
                     LAPVAALVYLWRLRPRRCRRYLVTSERLLLVEGLIARVVWDAPWSQISRLEVVTHPGQ
                     VALQAGDVQVYEGDRMLFTLPGVSLVENFIRICHRTQQAKLQVSQLRQRRRA"
     gene            complement(2781..2951)
                     /locus_tag="PKI05_05005"
     CDS             complement(2781..2951)
                     /locus_tag="PKI05_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51592.1"
                     /translation="MAQVSIGLSSNLGLGVSWFSKRIGLLRQEIRELLGHELAEIDKL
                     VQEMLGLAGAES"
     gene            complement(3451..>4372)
                     /locus_tag="PKI05_05010"
     CDS             complement(3451..>4372)
                     /locus_tag="PKI05_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA51593.1"
                     /translation="DYWNHRRHGINYLRENEQEIDPPGHATDLFTEWACQYIRRHHQT
                     QPNRPFFLYLAYNAPHVPIQPPEEALNRYRQRHPDAPEKRSKLAALIEHMDAGIGRVL
                     QTLDELGLTESTLVVFTSDNGGQLDVGANNGPWRSGKGTMYEGGIRVPMVVRWPNKIQ
                     ANSQSEAIALTMDLFPSLLEAAGVEPPTDRDGVSLLPIWLGKAEDLLARELFWVRLEG
                     NKAGFSYAIRRGKYKLIRPAPTKSLAPIEVFDQLYDLEADPYEKQDLAPQQPQLLSEM
                     RRLLENRIKKTIDVPWRDEQGLGPGEIAPQ"
BASE COUNT         1022 a         1303 c         1203 g          844 t
ORIGIN      
        1 cgccacggtc accgtcacca gaaaggcaac gcacatgacg gccgtgaccg ggatgcacgc
       61 ccgggccatc cggtcagcac gtcccagtga agcgtagatg gttcccgtaa cattgaaaag
      121 ggcctggaaa gggatcatga gggcgaagat tctcaggatc ggtcccgccc ctgaccagcg
      181 aggtccgccg agcacggcca tggtttcgtc cgccaccaga gccaacccaa tcccgcaggg
      241 aaacatgatc caattcaaga agcgaagaaa ccgagtagcg tactgccggt agagagaggc
      301 ctccgagcgg gcacgggcca aggccggcag catgacgccc gttagaggag aaaccacaag
      361 acccaccggt ttttgggcca gattaaaggc ctggtaataa agggccaggg cctgcgaacc
      421 gaggaaacgt cccacgacaa tcttatcgac atgactcata agataaagga gaaaggcact
      481 catcgtgtag tgaccgccaa agtgcaatag ttcacggcta ccggccccgc gaagcacaaa
      541 ggccggacgc catcccgtga ccacccagga aaggaccgtc aaagccgccg gctcggcata
      601 ttgctggacg acaagggtcc aaattcccgc ccctgcccac gccgcgatca aggcagccac
      661 acccccgatg acctgcgcgg caacccggat gatcgatagt cgtcccaatt gcaaatcccg
      721 ctgtaaaagg gcctggtgct gagcccccaa cgttgtgacg agcatcgtcc cggaaagggc
      781 aatcgttaaa gggacgagtt cctcccggcc ataaaaccaa ctaatccacg gcgcactgac
      841 cgcgacgatg gccgttccta acatgcccag gatttgattg atccaaaata gggcggaggc
      901 cctttggcga tcgagcaagg gatcctgaat ggctgccaca tcaaggccgg agtagataaa
      961 gatgcgaacg agcgccagga gaggaagtac catccccaat aggccataag gctccaagcc
     1021 caacgtccgt aacaaaatgg ccaggacaac cagggaaatt aattgagaaa caacctgggc
     1081 aacaaagacg gctgagctcc cgcgccgaac gtctcgctga agccgctcgg ccatcgcatc
     1141 aaggggtggc agctcctcaa atgccatcgt gcaattcccg cagatttcag gaaggacgac
     1201 ggggaacagg gcggcccatc acctggagga gcagcttcaa atcctcccaa gcctcccgtt
     1261 tcatggccgg gtttcgcaat aaaaacgctg ggtgatacgt acagacaacc tttgcccgat
     1321 cgtattccca gaccttcccc cggagctttc cgatcccaac atccgttcgg agtaggtttt
     1381 gggcggccac gttccccagg caacagatgt atttcggctg gatcaccgcc aacgtggcat
     1441 ccagccacgg ccgacaacgg tcggcttcct cgcgggtcgg cgttcgattg ttgggcggac
     1501 gacagcgaag aatgttacaa atatacacgt cctcgcgtcg catccccatc ccgttggtta
     1561 tcatagcggt caataattgc ccggcccgac cgacaaaagg ctctccttgc ttgtcttcat
     1621 caacacccgg cgcctctccg ataaacacaa gctctgaata cggattgcct acaccgaaaa
     1681 ccgtctgcgt ccggtttgca accaattccg gacaacgtgt gcactgtgcg acctgcttcg
     1741 ccaactcggc caaaacatct tcacgacttt cggacaatgg ttggatctcc cccaacggtg
     1801 ccacggtatc caccacagga agctgcccag ttacgcggtt ttcctctgat ccctgccttg
     1861 cctgagcttc caacggctca ctttggccca gacgagtgtc atcagaactc acctgttggg
     1921 atcccaataa cgaagaatca atcgtctcgg cggacggcca aatgagatgg ttgatccccg
     1981 catgttgcat gtcctcaaga tactgaacaa gcccccgtcg cagacgttcg ggatcgagcg
     2041 acggcgaatt catcttgtct tgctgggagg ccggtttcat ggcaagcagg aatgagtaat
     2101 tctcaaaggt tccaaatcac ttctcccgcc gagtatcccc gttgggtaag gtgtaccacg
     2161 ctgggaatgc ctatgttgct ggtaagtcgg tcatgatgac caacggctgc ctttatgccc
     2221 tgcgtctttg ccgaagctgg gagacctgga gcttcgcctg ttgtgtgcgg tggcagatac
     2281 ggatgaagtt ctcgaccaag ctcacgcccg gcaacgtaaa gagcatgcga tcgccttcat
     2341 acacttggac atcgcccgcc tggagcgcca cttgcccggg gtgagtcacc acttccagtc
     2401 ggctgatctg ggaccaagga gcatcccaca ccactcgagc gataagcccc tcaacgagga
     2461 gcaggcgctc gctggtcacc agatacctcc gacaccgcct cggccgaagt cgccacaaat
     2521 agaccaaggc cgccacgggt gccaaaaaga agccgacaca caatgcggcc aagggtccac
     2581 gcgccaaggc ccatccccat acccgaccca cccagcggcc taacggccag gccgtaatgg
     2641 agggccaaac gaccttgacc acttgttcat ttggcatcaa catctcggtt ctacctggct
     2701 ataaaaaatt tgacttctct tggcaaatgg gttcgatcgg tccattgtac cgggctccgt
     2761 tttaaaagga gagggcagga tcagctttcg gcgccagcca gaccgagcat ctcttgcacg
     2821 agtttgtcga tctcagccaa ttcgtgcccc aaaagctcgc gaatctcctg acgcaataaa
     2881 ccaatccgtt tgctgaacca tgaaacgccc aggccgaggt ttgaagaaag gccgatggac
     2941 acttgagcca ctgccggtga ccgctcaagc aattcgctca gacggtggag agaaacctcc
     3001 gataatggcc ctggcgtgcg tctagatcaa cgagtggcga gagaacaacc atgaaaggcc
     3061 acgggaaggc ccattgatta cccgtagcgt tctccaaatt cgagtctatc ccaaaaagtt
     3121 cccctctccc tctgggagag ggttggggtg agggcaagcg atcgtcgaag tcgaacgaga
     3181 aaaccggcag cgattcgagc gaccctcacc cccgacccct ctcccacagg gcgaggggag
     3241 attgtgggat aggttctttg tcacctgcgt tttcaactga tggagctcac aaattgccgc
     3301 atttctccat ctaaaattac aatttacccc aacgcctatt tcgatcacga ggtaaactcc
     3361 cgcggggtat ccctcggcag aagaccgaac ctagatggag aaaatggccc gacgacagcg
     3421 cctgacagtt ggatgaaaga aatgccacgg ttattgggga gcgatttcgc cggggccgag
     3481 tccctgttca tcacgccacg gtacgtcgat ggtcttcttg attctatttt cgagtaagcg
     3541 ccgcatctcg ctaagaagtt ggggctgctg aggggcgagg tcctgttttt cgtaggggtc
     3601 ggcctccaaa tcgtatagtt ggtcaaacac ttcgataggg gcaaggcttt tcgtgggggc
     3661 agggcggatc agcttgtatt tgccccgacg gatggcgtag ctgaagccgg ccttgttacc
     3721 ttccagacga acccagaaca attcccgggc gaggaggtcc tcggccttcc ctaaccagat
     3781 tggcagcagg ctaaccccat cacggtctgt cggcggctcg acaccggcgg cctccaggag
     3841 gctgggaaac aaatccatcg tgagcgcgat ggcctcgctt tgggagttgg cctggatttt
     3901 gttcggccag cggacgacca tgggaacccg gattccgcct tcgtacatcg tccctttacc
     3961 gctccgccat gggccgttgt tggcccccac atcaagttga cccccattat cgctggtgaa
     4021 aaccacgagc gtgctctcag tcaggcccag ctcgtcaagg gtttgcagga cacggccgat
     4081 gccggcgtcc atgtgctcga tgagggcggc cagtttcgac cgtttttccg gggcatcggg
     4141 atgccgctgg cggtaacgat ttaacgcctc ttcgggcggc tgaatcggca catgcggtgc
     4201 attgtaggcg agatagagga aaaaggggcg attgggctgc gtctggtgat gcctgcgaat
     4261 gtactgacag gcccattctg taaacaaatc cgtggcgtga cccggcggat cgatctcctg
     4321 ctcgttttca cggaggtagt taatcccgtg acgccggtgg ttccagtagt cg
//
LOCUS       DAOPTR010000194         4355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16248_length_4355_cov_3.570930, whole genome shotgun sequence.
ACCESSION   DAOPTR010000194 DAOPTR010000000
VERSION     DAOPTR010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16248_length_4355_cov_3.570930"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /locus_tag="PKI05_05015"
     CDS             complement(<1..1243)
                     /locus_tag="PKI05_05015"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOA51594.1"
                     /translation="MGRHGGYQSRRDFLRVTGLSWAIGQAGGIAVPSLLLGEENSGPI
                     DCGPPPKAAPQHRTGGESFPPLPLPVTPLRRSEKKRPPSPPALIGKMALGSVRWITRE
                     GKRVMYRDWMTDPADVNTLLEWTNEKLGIHYRAVETDFAHFSFDPRELPALLFAGHNS
                     FQLNDEVRAKLARYVLDGGTIIGDACCGWNDFAESFRKEMELIFPGRPLRKMLPEEPV
                     FSSYYKLGNFTYQKGDGSQYDDAPCLESIDFGCRSGVIFSPADMTCGWDGHDHPRGLR
                     LTIDLARQVGANLVTYLLGTYQLARFLSTTKVYYEAEAPTRDDFVFAQIIHDGDWDPD
                     PSAVHNLLKFAKDNSTLEVKFKRQNVRLTDPQVANYPLLYMTGHREFVWSDDEVAFLQ
                     RYLKAGGLLLADACCGRMSFDM"
     gene            complement(1361..3064)
                     /locus_tag="PKI05_05020"
     CDS             complement(1361..3064)
                     /locus_tag="PKI05_05020"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOA51595.1"
                     /translation="MSSFDREKLTLLIDRVLEGCASREEIRAIEEGARSADAQRFLAE
                     YLLLAGELHWLAIDDDLKDREILARVSLPEGTSDHGDRDLIARTAGSGPWLGGQFRKM
                     GLLVIAVTAVLLIGLISIWLRTGARLPSQGQFSTPVIAQLEKVYPEDSKKIGATIRPG
                     DWVIVREGAAQWQIRGGGSLIAQGSTRFRLLENGQVELESGTIFLRTKESGHLAGVIT
                     PQARYLDRGTAFGVLCDMTRSEVHVLEGEVEVIARRLGLEGSRRVVAGEALACDGRGR
                     TEPIPCRAERFCQMVPEALTVAAYRLMALSDPRLWLYGAFESPRFDGKWHAVLGPTDF
                     QPVWMRGTMSRLDVQQISGYDSQSRAVKLIRGTYSGDTVGVGLQTIDPVVLPRAMTVE
                     MIVRFDGWPSAGENTLACLLATRQDQRRCAVWLGVVPVERESRHLGRLVHLCDGMAPW
                     TETKGTLVPGHWYYVAATFEPERDATRVSTWLADLTEKEPLVCVLKDGQLAGRPVDGY
                     LGIGKGFDDGMSHAYPFPGAIDELAIYNGVLEEDLIRFHLTHLIGTKGQESVLPLPGG
                     P"
     gene            complement(3079..3630)
                     /locus_tag="PKI05_05025"
     CDS             complement(3079..3630)
                     /locus_tag="PKI05_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOA51596.1"
                     /translation="MREQQGELPPDEEFVRLLSGVQRKLFLFVFTLVPNVVEAEEIVQ
                     STSVVLWQKFSQFRKGEDFLRWACGVARNEVLKHRARASRHEATFSNEFMEQLAETTV
                     PLMEAVDERRQALQRCLEELQPHHRRLLAQRYQEGISTEEIAKNQGKTADAVRRMLHR
                     IRLAIYDCVQKKLASMEWGTSTP"
     gene            3924..>4355
                     /locus_tag="PKI05_05030"
     CDS             3924..>4355
                     /locus_tag="PKI05_05030"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOA51597.1"
                     /translation="MHKRILPVAILISGSGRTLRDLLKAVDEGRVPIEVRLVIASTPN
                     ASGLQHAEFRCIPAEVIERRDFNSAEEYSAAILRRCQEVGAEYVIMAGFAMLLTITPE
                     YQNRVIGSHPALAPAFCGQGYFGRRVHAAVLEHGVKVTGCTV"
BASE COUNT          993 a         1406 c         1019 g          937 t
ORIGIN      
        1 ccatatcgaa cgacattcgt ccgcaacagg catcggccag caacagacct ccagccttga
       61 gatatcgctg gaggaaggcc acctcatcat ccgaccagac gaactcacgg tgtcccgtca
      121 tgtatagcag aggataattg gccacctggg gatcagtcag tcgaacgttt tgccgtttga
      181 atttcacctc caaggtggaa ttatcctttg caaacttcaa caaattgtgg actgccgacg
      241 gatcggggtc ccagtcccca tcgtggataa tctgagcaaa aacgaagtcg tcccgcgtgg
      301 gggcttctgc ttcgtagtaa accttggtcg tgctcaaaaa gcgggccaat tggtatgtgc
      361 ccagcaaata ggtgactaag ttggctccaa cctgacgagc gaggtcaatt gtcaatcgca
      421 ggccccgcgg gtgatcatgc ccatcccaac cacacgtcat atcggcgggc gaaaagatca
      481 cgccagaacg gcacccaaaa tcgatcgatt ccagacaggg tgcgtcgtca tattgcgagc
      541 cgtccccctt ctggtacgta aaattgccta gtttgtaata tgacgagaag accggttcct
      601 cgggcagcat ttttcttaac ggccttcccg gaaagattag ttccatctcc ttgcggaagg
      661 attcggcgaa gtcattccag ccgcagcacg catctccgat aatcgtgccc ccatctaaca
      721 cataccgggc caactttgcc cgcacctcat cgttcaactg gaaactattg tgacctgcga
      781 acaaaagggc cggcaattcc cgcgggtcaa aggaaaaatg ggcaaaatcg gtctcgaccg
      841 cccgatagtg aatccccaac ttctcgttcg tccactcgag tagcgtgttg acgtctgcag
      901 gatctgtcat ccagtcccgg tacatcaccc gcttcccctc acgagtgatc catcgtacag
      961 atcccaaggc catcttaccg ataagcgccg gcgggctggg aggacgcttt ttctccgacc
     1021 gcctcaaagg cgttaccggc aaaggcagtg gcgggaatga ttcaccgccc gtccgatgct
     1081 gcggggcagc cttgggcggc ggaccgcaat caatcgggcc cgaattctcc tcaccaagca
     1141 gcagcgaggg cacggcgatt ccccccgctt gaccaatggc ccaactcaac cccgtgaccc
     1201 tcagaaaatc acgccgagac tgataaccac catggcgacc catagtttca cctctcatca
     1261 atgaacgact cgtcttctct gtccgacccg tcgtctgcca actcacacgc cttttgcctg
     1321 gtccgacccc gccgtcatga cacctcccat ttccaccgct tcacggccct cccggtaaag
     1381 ggagaacact ctcctgcccc ttcgtcccaa tcaagtgtgt cagatgaaaa cgaataagat
     1441 cctcctccaa cactccattg tagatggcca actcgtcaat cgcacccgga aatgggtacg
     1501 catgtgacat cccatcgtcg aatcctttgc caatccctaa ataaccgtcc actggacgcc
     1561 ccgcaagctg cccatcttta aggacacaca ccaggggctc tttttccgtc aagtctgcca
     1621 gccacgtgga cacccgcgtt gcgtccctct caggttcgaa ggttgccgcc acatagtacc
     1681 agtgcccggg gacgagagtc cccttcgtct ccgtccatgg tgccatcccg tcgcagaggt
     1741 gaactaaccg ccccaagtgc ctgctttctc tctcaaccgg gaccacgcct agccacactg
     1801 cacatcgacg ctgatcctgt cgtgtggcca agaggcatgc cagggtattt tcccccgccg
     1861 atggccagcc gtcaaaccgg acaatcatct ccacggtcat cgccctcggt aacacgactg
     1921 gatctattgt ctggagcccc acccccaccg tatcgcccga atacgtacca cgaatcagct
     1981 tcacggccct cgactgcgag tcatagccgc tgatttgctg cacgtctagc cgcgacattg
     2041 tgcctcgcat ccagaccggt tggaaatctg tcggtcccaa aaccgcgtgc cacttgccat
     2101 caaaacgcgg cgattcgaac gccccataca gccacagtcg aggatcagaa agggccatca
     2161 acctgtacgc ggccaccgtc aacgcctcag gcaccatctg acaaaaccgc tcggcgcgac
     2221 atggaattgg ctctgtccgc ccacggccgt cacaggccaa cgcctcacct gcaaccacac
     2281 gccgtgaccc ctccaggccg agccgtcgcg cgataacctc aacctccccc tccaggacgt
     2341 gcacctccga cctcgtcatg tcgcacaata cgccaaatgc ggtaccgcgg tccaaatacc
     2401 gtgcctgcgg tgtgattacc ccggccaagt gtccactttc tttggttctc aaaaaaatcg
     2461 tccccgattc cagctccacc tgcccatttt caaggaggcg aaaacgcgtc gatccttggg
     2521 cgatgagact tccaccgcca cggatttgcc actgggctgc accctcccgc actatcaccc
     2581 aatcgcctgg cctaatagtg gcaccaatct tcttagagtc ctccggatat accttttcca
     2641 actgggcaat caccggcgtg gaaaactgcc cttgtgaagg cagtctcgcc ccggttctta
     2701 gccaaattga gatgagacca ataagaagca cggcagtcac ggcaatgacc aacagcccca
     2761 ttttccggaa ttgcccgccc aaccatggcc cggaccccgc cgtacgtgca atcaagtctc
     2821 gatcgccgtg gtcacttgtt ccctcaggca gacttacgcg tgccagtatc tcgcggtctt
     2881 tcaaatcgtc atctatagca agccagtgca actcgcccgc gagcaaaaga tattctgcca
     2941 ggaatcgttg cgcatccgct gagcgagccc cctcctcgat tgcgcgaatc tcttcccggc
     3001 tcgcgcaccc ctcgagcacg cggtcgatca agagcgtcag tttttcccga tcaaatgaag
     3061 acatcgctcg ccgtcacctc atggggtact ggtcccccac tccatagagg ccagcttttt
     3121 ttgcacacag tcgtaaatag ccaaacgaat gcgatgaagc atccgccgca cggcatccgc
     3181 ggtttttccc tggtttttcg ctatttcttc agttgaaatc ccctcttgat accgttgcgc
     3241 caataaccgg cgatgatgcg gctgcaactc ctctagacat cgttgcaggg cctgacgccg
     3301 ttcatccaca gcctccatta aaggcaccgt tgtttcggca agttgttcca tgaactcatt
     3361 cgaaaatgtg gcctcatgac gacttgcacg ggcacgatgc ttcaatactt cattccgtgc
     3421 cacaccacag gcccatcgca gaaaatcctc ccctttgcga aactgactaa acttttgcca
     3481 cagcaccacg cttgtgcttt gaacaatctc ttctgcttcc accacattgg gaaccagtgt
     3541 aaacacgaac aaaaagagct ttcgctgaac gcctgaaagc aagcgtacaa actcttcatc
     3601 cggtggaagt tcaccctgtt gctcccgcac gtgctttcga gtcctcttcg agaatctctt
     3661 caccccgcac tagagaatca gaacgttttc tcgtgcctct ccctaataca atatcccgct
     3721 gactctgcca aaatcggaca acatttttcg cgagcaacca cctaatggtg gtaataagcc
     3781 cttaggattt ccgtagaggg ctgagacatg ttatcccaat ttcggtcacg ttacaattga
     3841 ccagtaatga ccgtttcacc cctagaagtg ggataattcc cgtgccagcc gaagataaga
     3901 gaaccattta aaggtcacta actatgcaca agagaatcct acctgtcgcc attttgatat
     3961 ccggtagcgg ccgaacgctt cgtgacttgc tgaaagccgt tgacgaggga agggtcccca
     4021 tcgaagtccg cctggtcatt gccagtaccc ctaatgcgag cggtctccag cacgctgaat
     4081 ttcggtgtat tccggcggag gttattgagc gacgcgattt caattccgcg gaggaataca
     4141 gcgccgccat cctccgccgt tgtcaagagg tgggggccga gtatgtcatt atggcggggt
     4201 ttgcgatgct ccttacgatt acgccggagt accaaaaccg cgtgataggt tctcatccgg
     4261 ctttggcacc agcgttttgc ggacagggct acttcggccg aagggtccac gctgcggtcc
     4321 tcgaacacgg cgtcaaagtt actggatgca cggtg
//
LOCUS       DAOPTR010000195         4343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16301_length_4343_cov_3.452425, whole genome shotgun sequence.
ACCESSION   DAOPTR010000195 DAOPTR010000000
VERSION     DAOPTR010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16301_length_4343_cov_3.452425"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PKI05_05035"
     CDS             complement(<1..302)
                     /locus_tag="PKI05_05035"
                     /inference="COORDINATES: protein motif:HMM:NF024646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51598.1"
                     /translation="MEHSRHGCGAVKRSCLNDRAAQPAGDGVSLPSAIRRAVSLARWH
                     LLNRQHTEGYWQGELEGDSILQSETILILAFFGRENSPLVARLAERLLETQSEKGG"
     gene            complement(487..1794)
                     /locus_tag="PKI05_05040"
     CDS             complement(487..1794)
                     /locus_tag="PKI05_05040"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA51599.1"
                     /translation="MKQPRGMMTRREMVKLSAVGLTVPTIIARTALGSEERLAPSERL
                     TLGFIGMGKQNQYHLNAFLGMKDVHVVAVCDVDTTRRNFSRDKVNAKYGNEDCAAYND
                     YRELIARKDIDAVVIATPDHWHTHPIVDACKAKKHIYCEKPLTLTIAEAKLCIEVVNK
                     YGVVFQTGSQQRSGREFRTACEAVRNGRIGKIKQVYASVGGPSKWCDLPEEPMEPGLD
                     WNLWLGQAPMRPYNSILSPRGMHDHFPAWRAYREYSGGGMTDWGAHHFDIAQWGLGMD
                     DSGPVEIIPPEQPEKGTGVRFIYENGVELIHGGYEGRGGVNFVGTDGVIYVDRGKLQS
                     WPEEVVKEPLKESEKVRLYESPGHHRDWLNAIRENRQPICPVEVGARSVTVCHLGNLA
                     YWTGKRLKWDPKNWRFVEPTEANEWLDGIRREPRRDPWQLPTI"
     gene            2156..3178
                     /locus_tag="PKI05_05045"
     CDS             2156..3178
                     /locus_tag="PKI05_05045"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOA51600.1"
                     /translation="MERNPARRPSQSPQESERNEISSAEIMDYFLDKVEELHSSPAVI
                     WRVLQLLKDPEFDVRDVELLLEADPALSAAILRLVNSSAYGLPQKITSLRQAILLLGA
                     RSLRLAVLSFGLVDRLTKGTPAKVYEDFWRRALTTASAAARLARTYRTTNADEAYCAG
                     LLSELGVLALAQVDTRRYTRVYLTHRHGEDLCPAEQARYGFDHAALGERLLLRWGLPR
                     PLAQVAGSHHLVEGTPEALHAVVHAGSLLADVLWMPQSVLLPHARQWLSDRFQIDLDG
                     FITLAMDCKQDILDNAAMFSIHLEGKIDIDLLRREAQRQFKQVALETSLEYDAMTAVL
                     NQEMGI"
     gene            3484..3873
                     /locus_tag="PKI05_05050"
     CDS             3484..3873
                     /locus_tag="PKI05_05050"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOA51601.1"
                     /translation="MIRFAWGKNGFWREERPSSQVVRLEELPAGERAQIVSLSGPEDV
                     VHRLEELGLRPGTVIRVFRPGNPCIVCLGGSKLGVRADGHVQILVSPLPGPPRCRHRH
                     GWGWRRAAIVSPTAPGKDVPDPLPPAN"
     gene            4003..>4343
                     /locus_tag="PKI05_05055"
     CDS             4003..>4343
                     /locus_tag="PKI05_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HOA51602.1"
                     /translation="MAVSADKRCITVALVGNPNTGKSTLFSALVGVYQRVGNYPGVTV
                     EKRTGRFEFAGQSFEVTDLPGLYSLAPRSRDELAAVDVLLGREPGVEPIDIIVCVVDA
                     SNLERNLYLVS"
BASE COUNT          886 a         1311 c         1193 g          953 t
ORIGIN      
        1 cagccgcctt tttctgattg ggtctccaga aggcgttcag ccagccgcgc taccaatggg
       61 gagttttctc gcccaaaaaa ggccaggatg aggatcgttt cgctttgcag gatgctgtcg
      121 ccttccagtt caccctgcca atagccttcc gtatgttggc gattcaacag atgccagcgg
      181 gcaagagaaa ccgctcgccg aatcgccgat ggcagggata ccccatcgcc ggcgggctgc
      241 gctgcgcggt cattcaagca tgatcttttt acggccccac atccgtgccg agaatgttcc
      301 atggccaatg cttcctttgt gacgcactcc gagacgtcca agtcaataga tagattaaag
      361 gagacgcgca aagttggcaa gacgaacttg acggacgcga aacgcgctgg gtccgcacta
      421 ggattgcgcc ttcggtccct cattccccac aatcgccgcc tacatgtcaa gccaggatga
      481 gggccatcaa atcgtcggca attgccaagg atcccgtcgc ggttcgcggc ggattccgtc
      541 caaccactcg ttggcctcgg ttggttcgac aaaccgccag ttcttggggt cccacttcag
      601 ccgttttccg gtccaataag ccaggttccc aagatggcaa acggtgacgg atcgggcacc
      661 aacctcaacg gggcaaatcg gttgccggtt ttcacggatg gcattgagcc aatcacgatg
      721 gtggcccggc gactcataca gacgcacttt ttcgctctct ttgagcggtt ccttgaccac
      781 ctcctcgggc caactttgaa gtttaccgcg atcgacatag atgacgccgt ccgtacccac
      841 aaaattgact ccgccgcggc cttcgtaacc accgtggatc agctcgacac cattctcgta
      901 gatgaagcga acgcccgttc ctttctcggg ttgctcgggc gggatgatct cgacagggcc
      961 cgagtcgtcc atgccgagtc cccactgggc gatgtcgaaa tggtgagcac cccagtcggt
     1021 catccctccg cccgagtact ctcgatatgc ccgccatgcc gggaaatggt catgcatacc
     1081 tcgcggactg aggatcgaat tatacggccg catgggtgcc tgccccagcc ataaattcca
     1141 gtccaacccc ggttccatgg gctcctccgg cagatcgcac cacttgctcg gtccgcccac
     1201 agaggcgtaa acctgcttga tcttgccgat gcgaccattc cgcacagcct cgcacgcggt
     1261 tcgaaattct cgacccgacc gctgctgact ccccgtttga aacaccacac catatttgtt
     1321 gaccacctcg atgcacagtt tggcttcggc gatcgtgagt gtgaggggct tctcgcagta
     1381 gatatgcttc ttggccttgc aggcatcgac aatgggatgg gtgtgccagt ggtcgggtgt
     1441 agcaataact acggcgtcaa tatccttgcg ggcaataagc tcacgataat cgttatacgc
     1501 ggcgcaatct tcgtttccgt atttagcgtt caccttgtcc cgcgagaaat tccgccgcgt
     1561 agtatcgaca tcacaaacgg ccaccacgtg gacatctttc atccccaaga aggcgttcaa
     1621 gtggtattgg ttctgcttgc ccatcccaat gaagcccagg gtcaatcgtt cgctcggtgc
     1681 caggcgttct tcgcttccca gggctgttcg ggcaatgatg gtgggcacag ttagtcccac
     1741 cgccgacagt ttgaccatct cccgccgcgt catcatccca cgtggttgtt tcatcgtcgt
     1801 tacctcccga ttgtttgcta taagccccca tcaaaaaacc cacccggcga cccctcctca
     1861 cggccttagt gcctcccaca tccctgggct tggggcgcaa gttccctgac aaaatggacc
     1921 acagatcact gcgcaagtta atcactcaga taggcaaaaa caagtggcgc ccgcccgaag
     1981 cggcgttttc gaaggcctct tgcgcccaac gcactgctag gcatgaaaaa ggctcctccc
     2041 tccggggtcc gaaacattgg gcattttcct cggaaggtga gctatagttt cattagatgc
     2101 tagacggcga agtcattcat gaacgttcta gggacaaaaa gagatcaatt gagccatgga
     2161 acggaaccca gcacgacgcc catcacagtc gccccaagaa agcgagcgaa acgaaatcag
     2221 ctccgccgaa atcatggatt atttcttgga taaggtggag gagctgcatt cttctccggc
     2281 ggtcatttgg cgggtccttc agcttttgaa agaccccgag ttcgacgttc gcgatgtgga
     2341 actgctgttg gaagccgatc cggccctgtc agccgccatc ctacgattgg tgaacagttc
     2401 ggcctacgga ctaccgcaga agatcaccag cctgcgacaa gccattctcc tcctgggtgc
     2461 gcgttccctt cgcctggccg tcctcagctt tggtctggtt gatcgcttga ccaaaggaac
     2521 gcccgccaaa gtgtatgaag atttctggcg acgcgcactg acaacagcct ccgccgcggc
     2581 tcgcctcgcc aggacctatc ggaccacaaa tgccgatgaa gcctattgcg caggattgtt
     2641 atcggaactc ggcgtcctgg cactggccca agtcgatacg cggcgttaca cccgggtgta
     2701 tttgacccac cggcacggcg aggacctttg ccccgcggaa caggcccgct atggctttga
     2761 tcacgccgcc ctcggcgaac ggctgctcct tcgctggggg ttgccccgac cgcttgccca
     2821 ggtggccggt tcccaccacc tcgtggaagg cacgcccgag gcccttcacg cggtcgtcca
     2881 tgccggttcg cttctcgcgg atgtcctttg gatgccgcag tccgtgctcc ttccccacgc
     2941 ccggcagtgg ttgtccgacc gcttccagat cgacctggat ggcttcatca cgctggccat
     3001 ggattgcaag caggacatcc ttgacaatgc cgcgatgttc tccattcact tggaaggtaa
     3061 aatcgacatc gacctgctgc gtcgggaggc ccagcgacaa ttcaaacagg tcgctctcga
     3121 gacgtctctc gagtacgacg ccatgaccgc cgtgctcaat caagaaatgg gaatctgaag
     3181 agacgcatcc cgcacggcca acttgcgcta cgggcttgat ctagcgcccg tttcttctca
     3241 gcttggggtg gcaaacgcga aagtgggcaa accccacgcc atccgtgctt gctttgttcc
     3301 gatggagaac gtatgataaa gaggtcataa atgagactga gtctcaataa gccctcgcta
     3361 ttgagactgg gacaaaatac ggcatggcgg atcggtcgtt aggcatcgga tttgtttggg
     3421 caaaccctcg gtgcggtcgg ctgtcatttt ggcgtgacat tatctccatc ccatgaggat
     3481 atcatgatca ggttcgcttg gggaaaaaac ggtttctggc gcgaggaaag gccttcctcc
     3541 caagtggtgc ggcttgaaga gcttcccgcg ggagaaaggg cccagatcgt ttctctcagc
     3601 ggacccgagg atgttgttca ccgcttagaa gagttggggt tgaggccggg aacggtcatc
     3661 cgcgtctttc gtcctggtaa tccgtgcatc gtgtgtcttg gtgggagcaa gttgggcgtg
     3721 cgggcggatg ggcacgtcca aatcctcgtg agccccctgc ctggaccacc gcggtgccgt
     3781 caccgacatg gctggggctg gcgccgtgcg gcgatcgtat cccccaccgc gccggggaag
     3841 gacgtccccg accctctccc tccggcaaat tgacggtcct gcagggcgat aacctgatca
     3901 cgacgggaat ccccatattg attgcccgct cattttcacc accgtcgtgt tgtctaaaga
     3961 aagatcgcac ggtgctccgc ttgggtgttt gcgacgaagt ttatggctgt ttccgcagat
     4021 aaacggtgca tcacggttgc cctggtggga aatcccaaca cagggaaatc gacgcttttc
     4081 agcgcccttg tgggggttta tcagcgggtg gggaactacc ccggtgtgac agtagagaag
     4141 cggacaggcc gattcgaatt cgccggacaa tcattcgagg tcaccgacct ccccggtctg
     4201 tacagtttag ccccacgatc ccgggatgaa ctggcggccg tggatgtcct gctgggacgc
     4261 gagccagggg tggagccaat cgatatcatc gtctgtgtgg tggacgcatc taatctagag
     4321 cgtaatcttt accttgtcag cca
//
LOCUS       DAOPTR010000196         4321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16406_length_4321_cov_2.619081, whole genome shotgun sequence.
ACCESSION   DAOPTR010000196 DAOPTR010000000
VERSION     DAOPTR010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16406_length_4321_cov_2.619081"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PKI05_05060"
     CDS             <1..240
                     /locus_tag="PKI05_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51603.1"
                     /translation="RVWVRVILQEKPNVLLVPRISVVREETDRWVVWVWNKRRVERRV
                     VTVGIMNDSFAEILSGLAEGEEVVLTPETGGAASH"
     gene            363..1193
                     /locus_tag="PKI05_05065"
     CDS             363..1193
                     /locus_tag="PKI05_05065"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HOA51604.1"
                     /translation="MGIRFYCPNGHKLHVKAFQAGMRGICPYCGAKVQIPLHSTRPST
                     KELKARRAAGGPEHAAIPRGDTAMPESRQPGVDSSPEKAPDGQGVLPGEQEQLTPFID
                     LDKVLAARNLGDQGGLRSSPSPLEGDLAISTAESVRSRNSVTEEWGSPGESARSFEKD
                     PLAEPNVVWYVRPPSGGQYGPAAPEVVRTWIAENRITPDTYVWREGWRDWQLARDVFP
                     EVLAKALPEVPVEDQPERRLIRRSVRSSWAQYAIVAFAAIVICVLGGLVWLFVSRSSP
                     "
     gene            1354..2247
                     /locus_tag="PKI05_05070"
     CDS             1354..2247
                     /locus_tag="PKI05_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOA51605.1"
                     /translation="MTDAPKQNRRGFLVSSGITAATLMMAPSVVPTTAQAKEASTPKA
                     PRFRLALASYTLRLFPLDQALAMTKRVGLKHICLKSFHLPLDASADQIEAAKKQVAEA
                     GIDLYAGGVINMATEAEVDQAFQYAQLAGMRVIVASPRPPVLERVNAKVKETGIAVAI
                     HNHGPGDKFFPTPESVIEQVKNLDKRIGLCMDVGHTARIGADPIQDARRFADRLLDVH
                     IKDINVAAPEGRCVECGRGVLDLPKFLKVLIEIDYAGMVAFEYEKDEKDPLPGLAESV
                     GYVRGVLDSLLGISDRSAQLE"
     gene            complement(2313..3020)
                     /locus_tag="PKI05_05075"
     CDS             complement(2313..3020)
                     /locus_tag="PKI05_05075"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOA51606.1"
                     /translation="MGDPEILYEDNHLLAVNKPAGLATMGLPEDQETLLSWLKSWLKR
                     RDAKPGNVYLGVVSRLDVPVSGVVLIAKTSKAAARLTEQFRTRSVSKIYWAIVEGRVP
                     QPAGTLRHFLRSDERFRRVHVTHAHDPDGQEAVLSYKQLAASRGLTLLEVHPHTGRKH
                     QIRIQLAVTGCPILGDQKYGAKQAFAEGIALHSRRIEFTHPVRKESMAIEAPLPVSWA
                     KLPFAWSEISSRSFRHE"
     gene            complement(3208..3798)
                     /gene="hisB"
                     /locus_tag="PKI05_05080"
     CDS             complement(3208..3798)
                     /gene="hisB"
                     /locus_tag="PKI05_05080"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOA51607.1"
                     /translation="MTPRTSQIERKTLETEIAASINLDGKGESRIDTGIGFFDHMLTL
                     FAKHSCNDLEIRCRGDLHVDQHHTVEDTGIVLGTCFRNALGDKKEIRRYGHWTLPMDE
                     VLATAAVDFGGRAYFVFNAQFSRETIGNFETELIREFWQAFAHCAEANLHICVHYGTN
                     NHHIAEAIFKATARAIRMAIERDPRGEGIPSTKGVL"
     gene            complement(3814..>4321)
                     /locus_tag="PKI05_05085"
     CDS             complement(3814..>4321)
                     /locus_tag="PKI05_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOA51608.1"
                     /translation="DEAYVDFAEESCLDLVRDYEHVMIARSLSKASSEVGLRFGFLIA
                     QPHIIRELRKVKDSYNCDALAIAGATAALSDQEWLQETRRKILATRERLMRELRGLGF
                     DVLPSQANFVWCSHPERASKEIYERLKARKVLVRYMNYPGWGDGLRITVGTDAEVDGL
                     LNSLREIL"
BASE COUNT          951 a         1145 c         1241 g          984 t
ORIGIN      
        1 cgcgtctggg tccgggtcat cctccaggaa aagccaaacg tgcttctcgt accgagaatt
       61 tccgtggtcc gcgaggaaac tgatcgttgg gtcgtttggg tatggaacaa gcggcgcgtg
      121 gagcgtcggg tcgtcaccgt tggcatcatg aacgactcct tcgcggagat tctttctggc
      181 ttggccgagg gcgaagaagt cgtgttaacg cccgaaacgg gaggtgcagc atcgcattaa
      241 tgattaatac aaagtacaat gaatagtagt gtgccagcgg taggttgcat cccaattgcg
      301 ggggagttac gcggccagtc gtggacttgg tggcttgccg cgatcggcgt caggcgtcat
      361 ccatgggaat acgcttttat tgtccaaacg gccataagct ccatgtgaag gcctttcaag
      421 ccgggatgcg gggaatttgc ccatattgtg gggcaaaagt gcaaatcccg ttgcacagta
      481 cgcgacccag cacaaaggaa ttaaaggccc ggcgagccgc aggtggtccg gagcatgctg
      541 cgattccgag gggcgacaca gcaatgcccg aaagccggca acccggtgtg gactcatcgc
      601 cggaaaaagc cccggacggg caaggggtgc tgcccggtga gcaagagcaa ctcacgccgt
      661 ttatcgactt agacaaggtg ttggcagcac gtaatcttgg tgaccaaggg gggctgcgat
      721 cctcaccaag cccattagag ggcgatttgg cgatttccac ggcagagagt gtacggtctc
      781 gcaattccgt gaccgaagaa tggggttcgc caggtgaaag tgccaggagt ttcgaaaaag
      841 atcctttggc agagccgaat gttgtctggt atgttcggcc tcccagcggg ggccaatacg
      901 gcccggcagc acccgaagtc gttcgcacgt ggattgcaga aaaccggatc acacctgata
      961 cctatgtctg gcgtgaaggc tggcgagatt ggcaattggc gagagatgtc tttcctgagg
     1021 tgcttgctaa ggcccttccg gaagtccccg ttgaagacca gccggagcgc cgtctcatac
     1081 gcaggtctgt gcgtagttca tgggcacaat acgctattgt ggcgtttgct gcgattgtca
     1141 tttgcgttct gggcggtctg gtttggttat tcgtctcgcg cagctctcca tagcggtcag
     1201 ttgggggcag cccccgccta ttgacggcta gattccaaga agaataatgg gaacctgagc
     1261 gacggtttgg cagggcccgg tggcatgtct ctgtaaccgt ttttcaacct ccgacgtgat
     1321 ctgagacacc tcttgtatga aggagttttc tcaatgacag atgcacccaa acaaaaccgg
     1381 cgtggttttc tcgtcagttc gggaattaca gccgcaacgt tgatgatggc tcccagtgtt
     1441 gttcctacga cggcacaagc taaagaggcc tcaaccccga aggcgccgcg atttcggttg
     1501 gcccttgctt cctatacatt acgtctcttc cccttggatc aggccttggc catgaccaag
     1561 cgggttggct tgaagcatat ctgccttaag tcgttccact tgcccttgga tgcctcggct
     1621 gatcagattg aggcggctaa gaagcaggtg gctgaggcgg gtatcgacct ctacgctggt
     1681 ggcgtcatca acatggcaac ggaggccgag gttgatcagg catttcaata tgcccagcta
     1741 gcggggatgc gggtcatcgt ggcttcgcca cgtccccccg ttcttgaacg tgttaatgcc
     1801 aaggtcaaag agacagggat cgcagtggca attcataacc atggcccagg ggataagttc
     1861 ttccccaccc ctgaaagcgt aattgagcaa gtgaagaacc tggacaagcg aattgggctg
     1921 tgcatggatg tcggtcatac ggcccggatt ggagcagatc ccatccaaga tgcccgccgt
     1981 tttgccgaca ggcttttgga cgtgcacatt aaggatatca acgtagctgc gcccgaaggc
     2041 cgttgcgttg aatgcggacg aggtgtcctc gatctgccaa aattcctcaa agtgctcatt
     2101 gaaatcgact atgcaggaat ggtggctttt gaatacgaga aggatgaaaa agatccgttg
     2161 ccgggattgg ccgaatccgt aggttacgtg cgaggtgtgc tcgattccct gttgggcatt
     2221 tcggatcggt cggcccaatt ggaataatgc cgcgaggttt tatccccgaa aaagtttgcc
     2281 ctttgctcgg gtgtcttggg gtcaaacgta agttactcgt gtctgaatga tcgtgagctg
     2341 atttctgacc aggcaaacgg aagtttggcc caactcacag gaaggggggc ctcgatcgcc
     2401 attgattcct ttcggacggg atgtgtgaac tcgatccggc gcgaatggag ggcgatccct
     2461 tccgcaaagg cctgttttgc gccgtatttc tggtccccaa ggatggggca gcccgtaacg
     2521 gccagttgga tgcgaatctg gtgcttccgt cctgtgtggg gatgcacttc gagcagtgtc
     2581 aacccgcggg acgctgcaag ttgtttatac gaaagcacgg cttcctggcc gtcagggtcg
     2641 tgggcatgcg tgacgtggac tctgcgaaaa cgttcatcgg atcgcagaaa gtggcgcagt
     2701 gtgcccgcag gctgaggaac gcgcccttcg acgatggccc aatatatttt cgagacactg
     2761 cgggttcgaa actgctcggt caagcgcgcc gcggccttgg acgttttggc aatcaagaca
     2821 actccgctga cgggaacatc gaggcggctt acaaccccta aataaacatt tccgggcttt
     2881 gcatctcgtc ttttgagcca gctctttaac caactaagaa gtgtctcctg atcctcgggt
     2941 aaccccatgg ttgccaaacc cgcaggcttg ttcacagcca aaagatgatt gtcttcatag
     3001 aggatttcag gatcgcccat aaccaatcat tcggcgatga ctagttgcaa catttccaca
     3061 ctgccttctg gggtgctagc gccagccgcc cagggcaagg aaaaaatggg acaccgtgca
     3121 caagcgtttg tcgaacgaat cttgaagatc cttcgcgcgg gccgaggtga acacccacga
     3181 catgaccacg gtccgctaac cgtacgttca caagacccct tttgtgctgg ggattccttc
     3241 cccgcgggga tcgcgttcaa tagccatgcg aatagcccgg gctgttgctt taaagatcgc
     3301 ctcggcgata tggtggttat ttgttccata gtggacgcag atgtggagat tggcctcggc
     3361 acagtgggca aaggcttgcc aaaattcccg gatcagttcc gtctcgaaat tgccaatggt
     3421 ctccctggaa aactgggcgt tgaagacaaa atacgctcgc cctccaaagt ccacggcagc
     3481 ggttgccaat acctcgtcca tgggcagtgt ccaatggccg taacggcgga tctccttctt
     3541 gtcgccaagg gcgttgcgaa aacaggtgcc caacacaatg ccggtatcct ccaccgtgtg
     3601 gtgttgatcg acgtgtaaat cgccgcggca tctgatttcc aaatcgttgc acgagtgctt
     3661 ggcaaatagc gtcagcatgt gatcgaagaa gccgatcccg gtgtcgatcc gtgactcccc
     3721 tttgccgtca aggttgatgg aagcggcaat ctcggtttcc agcgttttgc gttcgatttg
     3781 gcttgtgcgc ggtgtcatat tgctatcatc caatcaaaga atttcgcgga ggctgttgag
     3841 cagaccatcg acctcggcgt ccgtgcccac ggttatgcgc agcccatccc cccatcctgg
     3901 gtagttcata taccgaacga ggactttccg ggctttgagc ctctcgtaaa tctccttcga
     3961 ggcacgctcg ggatgcgaac accaaacaaa gttagcctgc gaggggagca cgtcaaatcc
     4021 cagaccccgc aactctcgca tcaaacgttc tcgagtcgcc aagatcttcc gccgcgtttc
     4081 ctgaagccat tcttgatcac tcagggccgc ggtggctccg gcaatagcaa gcgcgtcgca
     4141 gttgtaagaa tctttgacct tacgtagttc tcggataatg tggggctggg caatcaagaa
     4201 accaaacctc aagcccactt cactggaagc tttgctcaag ctccgggcaa tcatgacgtg
     4261 ctcgtagtcc cgaaccaagt caaggcaaga ctcttcggca aaatcgacat aagcctcatc
     4321 a
//
LOCUS       DAOPTR010000197         4320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16413_length_4320_cov_3.469871, whole genome shotgun sequence.
ACCESSION   DAOPTR010000197 DAOPTR010000000
VERSION     DAOPTR010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16413_length_4320_cov_3.469871"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="PKI05_05090"
     CDS             <1..1202
                     /locus_tag="PKI05_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51609.1"
                     /translation="KTYTHMINTMLSPGFRGIVVAALLAALMSTVSGALNSIATLFSY
                     DIYKRWVGEVSDRHLVWVGRIATFVAMCIAIAWTMALGRRDTTIFQAMVDVFPVVAPP
                     TAVVFLWGVFWRGTSGRAAFITLVGGSLIGLLVFYLTVIKVITINSLFMAFILFVVES
                     VGIFLLSLIFPHHHTAESESLVWKRPWDALRAENAWRGLMDFRVFAALLVITMVALYW
                     WFTSPHVYYPIRAKILVDGQPAPGVEVTLDADDDRLDDVLVADADGSVTYGTVMRAGG
                     APEGTVFRVAVRPARDYIVELRPRKNGEGIHNEDVLILAALPHGTEVAEELAGEYKVY
                     RWEAEGQKKEIRVPASAKVEIRRSPDIPARYRDPSQSGITVVALPEVQVQEVNIFTRE
                     VALAERP"
     gene            1318..1533
                     /locus_tag="PKI05_05095"
     CDS             1318..1533
                     /locus_tag="PKI05_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51610.1"
                     /translation="MVQIDLSTEEWQLILELLERERADLHCEVRHARFYDVRELLRQK
                     REAVEKLIERIESHLGGSVEVAAPEAG"
     gene            complement(1629..2243)
                     /locus_tag="PKI05_05100"
     CDS             complement(1629..2243)
                     /locus_tag="PKI05_05100"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOA51611.1"
                     /translation="MQDLILEFLHKFMLCFVPLFAAVDAAGLLPLYLALTDGLSPTVK
                     FRVLRQMLLTAILVVVAFVFIGRMILDFLGVTISDFMVAGGTMLFIVAMGDLFVVDKG
                     RPHVDTESLGAVPLGVPLTVGPAVLTTVLLLANAYGRLPALTAAVVNVVLAAIAFSWA
                     ANIEKLLGRNGVRALSKIASLFIGAIAVMMVRKGVFEMIASLQN"
     gene            2473..3498
                     /locus_tag="PKI05_05105"
     CDS             2473..3498
                     /locus_tag="PKI05_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51612.1"
                     /translation="MMLEPGDAHDSSVPLPDGGRRAVRSPRTFERSPTTFARSRESLS
                     RHPLDAALAFLRWRFGSEFLRSSAHLFLICGKYGLLVGAVLVVFQCLQKAGGQAWAIL
                     IGISLALLILVFHYIGGRFLFVLERWDRQINGYLSSSVVPDALSLLSLVGSSAMLVVG
                     AIDALITGSFELVFWGVAVFIWGLFVALQSLNLEGMNLQVSPELSPADEILGLIQFGL
                     KILVRLTPVVFGVMVLVGNLQLVMEIIIDGPELNRPEAYAAVGQQASDGKLVQASLDV
                     REPPFRVLRGRETLVMAGVLPGAMYLIYVLGHFASAIFSVFLAAVPENARGGMMTDHP
                     GKNDSTG"
     gene            3891..>4320
                     /locus_tag="PKI05_05110"
     CDS             3891..>4320
                     /locus_tag="PKI05_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51613.1"
                     /translation="MKLSYLSLAMLLSFVGLSANAAQAQPVVWIETSTLRICRSETPP
                     PSPSREVNLTAARNEWVSFQVFLRSSHPLPAVGVELSPLEGQSGGNIDSACFRIYREH
                     QLELKEPTYRNDAFRADWYPDPLIPARHPLTGEPLSGPRFT"
BASE COUNT          907 a         1104 c         1254 g         1055 t
ORIGIN      
        1 aaaaaaccta tacacacatg atcaacacga tgctcagccc tggttttcgg ggaattgtgg
       61 tggccgccct gttggcggct ttgatgagta cggtttcggg cgcactgaac tccattgcga
      121 cgttatttag ctatgacatc tacaagcgat gggtgggcga ggtgagcgat cgccatctgg
      181 tttgggtggg acggatcgcc acctttgtcg ctatgtgtat tgctattgcc tggacaatgg
      241 cgttgggacg ccgcgacacg accatctttc aggccatggt cgatgtcttc cccgtggtgg
      301 cacctcccac tgccgttgtc ttcctttggg gggtattttg gcgggggaca tcgggccgtg
      361 cggcgtttat cacacttgtg ggcggttcgt tgattggcct cctcgtgttc tatcttaccg
      421 tgatcaaggt catcacgatc aatagtcttt tcatggcgtt catccttttt gtggtggaat
      481 cggttggaat ctttctcctc tcgctgatct tcccccacca tcacacggcg gagagcgaaa
      541 gccttgtatg gaagagaccc tgggacgctt tgcgggctga gaatgcctgg cgtggcctga
      601 tggattttcg cgtctttgcg gccttgctgg tgatcaccat ggtggccctc tattggtggt
      661 tcacaagtcc ccatgtctat tatcccattc gggcgaagat ccttgtcgat ggtcaacccg
      721 ctcccggggt tgaagtcaca ctggatgctg atgacgatcg cttagacgac gtgctggtgg
      781 ctgatgctga cgggagcgtc acctacggga cggtcatgcg agccggagga gcaccggaag
      841 gaacggtctt ccgagtcgct gtccgacccg ccagagatta catcgttgaa ttgagaccaa
      901 ggaaaaatgg ggaggggata cacaacgagg acgttctaat cttggctgca ttgccccacg
      961 gaacggaggt cgcagaagag ttggcgggag aatataaggt ttatcgatgg gaagcggagg
     1021 ggcaaaagaa ggaaattcgc gtccctgcct cagccaaagt agaaatccgt cgatcgcccg
     1081 atatcccggc gcgatatcgt gatcccagcc aatcggggat aaccgtggtc gcgttgcccg
     1141 aggtccaagt ccaggaggtt aatatcttca cgcgggaagt cgccctcgca gaacgaccgt
     1201 aaatctccct atcagagagg cgtgttccgg cgagttgctc cccttgtcgg aggcatgtcc
     1261 cgtttttacg attctcgtgg ggctgatttg gcttttcctg gtacgggagg tgcggttatg
     1321 gttcagatcg atctctccac cgaagaatgg caactgatct tggagcttct ggagcgggaa
     1381 cgcgcagacc tccattgcga ggtccggcac gctcggttct acgacgtccg tgagctgctc
     1441 cgccaaaagc gcgaagccgt cgagaagtta attgagagga tcgaatcgca tcttggcggt
     1501 tccgttgagg tggcggcccc tgaggcgggt tgactccgtt agtctttcca acgtgggccg
     1561 cgacaaagcc gggtaatgct ctttttgtaa ttgcccccac taacttttga tcggagggcc
     1621 gggtgtctct aattctgcag cgaggcaatc atttcgaaga cacctttgcg gaccatcatc
     1681 acggcaatgg ccccaatgaa cagggaggct atcttggaca atgccctgac cccgtttcga
     1741 cctaaaagtt tctcgatgtt ggcggcccaa gagaacgcga tggcggcaag aaccacgttg
     1801 acgacagcgg ccgttaaggc aggaagccgt ccataggcat tggcaaggag gagcaccgtg
     1861 gtcagcacgg caggcccaac ggtgaggggg acaccaagcg gtacggcccc tagcgattcg
     1921 gtatctacat gcggccgccc cttatcgacg acgaacagat cgcccatcgc cacaatgaaa
     1981 agcatcgttc caccggccac cataaaatcc gagatcgtga cacccaggaa atcgagaatc
     2041 atccgtccaa taaagacgaa tgccacaaca acgaggattg cagtcaagag catttgtcgg
     2101 agaacgcgga acttgactgt gggcgacaag ccatcggtaa gggccagata aagcggcagt
     2161 agccctgctg catcgacagc cgcaaaaagg ggaacaaaac agagcatgaa tttgtgtaaa
     2221 aattccagga tcaaatcttg cacaggatgg aaccctaggt gattttagtc cgatgtcagg
     2281 gttttatgga ccagtccggc actccgaaag tacatgcaaa gaatctcgag atctaccgtt
     2341 tgttattctg acaaatgata atcacaatgg acgttatgga aaggtgggga agcggggaaa
     2401 gcgtgatcat aaacgctcgg gtttttgtca ctgtcatccg gaatgcccgg agcgcaacga
     2461 ggtcggccgg gaatgatgct cgaaccaggg gacgctcacg attcgtcggt gccactaccg
     2521 gacggtggtc gtcgtgctgt gcggtcgccg cgaacatttg aacgctcgcc gacgacgttt
     2581 gcgcgatcac gagagagcct gagtcgtcat ccgcttgacg cagctttggc gtttctccgc
     2641 tggcggttcg gaagtgaatt tcttcgttca agtgcgcact tgtttcttat ctgtgggaaa
     2701 tatggcttac tggtgggcgc cgttttggtc gttttccaat gccttcagaa agccggtgga
     2761 caagcgtggg caatcttaat tgggattagc cttgccttgt tgattcttgt gtttcactat
     2821 atcggcgggc ggttcctgtt tgtcttagaa cgatgggatc ggcaaatcaa tgggtatctc
     2881 agttcctccg ttgttcccga tgccctgtcc cttttgtcgt tggtcggttc atcagctatg
     2941 ctcgtggtgg gggctatcga tgccttgatc actgggtcat ttgagctggt gttttggggc
     3001 gtagcggtgt tcatttgggg actatttgtc gcgttacaat ctctcaacct ggagggtatg
     3061 aacttgcagg ttagcccgga gctatcgccc gcggacgaga tccttggttt gatccagttt
     3121 ggactaaaaa tccttgtgcg gttaaccccg gtcgtgttcg gcgtcatggt tctagtgggt
     3181 aacctgcaac tggtgatgga gatcattatt gacggtccgg agttgaaccg cccggaggcc
     3241 tacgccgcag ttggccagca agcttcggat ggaaagctgg ttcaagcttc gctcgacgtt
     3301 agggaaccac cgtttcgcgt attacgggga cgagaaacgc tggtgatggc gggtgtactc
     3361 cctggggcaa tgtaccttat ctatgtcctt gggcatttcg cctcggctat tttttcggtc
     3421 tttctcgctg ctgtgccaga aaatgcccgc ggtggaatga tgactgatca tcccggcaag
     3481 aacgattcga cggggtaata accctcgaag attgccgtca gcgtttgatt cgcgagtccg
     3541 tttctggact agcccgaacc tgcttccttg ttacggattc ttcctgcagg cgacgcggta
     3601 tctctcgaag ccaacgtctt ggcttgaact attgaaagat tcagaagcga ctatcagaca
     3661 acgagttgca acaaacagct aggcatgtgg ctagttcacg gatgcaccga cgaacggggc
     3721 acgcttccac ctccatattg cagggaccaa cggcgaatgg gtatcctggg gtttgcaaaa
     3781 gccggaagat acgtctgccg cgtgtttccc gagcagtttc caaaccttga tggcgtgaat
     3841 ctggcagtgt gtcccacctt gtaatccttt ggcttttgga gggaataacc atgaagcttt
     3901 cttaccttag cttggcgatg ttgttatcct tcgtagggct ctcggctaat gcggcacagg
     3961 cccagcccgt tgtttggatt gagacgtcaa ccctccgaat ttgccgcagc gaaacacccc
     4021 cgccctcccc ttctcgggaa gttaatctga cggcagcgcg caatgaatgg gtgagtttcc
     4081 aggtgtttct gcggagttca catccgcttc ccgctgtcgg cgtggaattg tcgccgttgg
     4141 aaggccaatc gggaggcaac atcgactcgg cctgttttcg tatctatcga gagcatcaat
     4201 tggagttaaa ggaaccaaca taccgaaacg atgcctttcg ggccgactgg tatcccgatc
     4261 ccttgatacc ggcacggcat cctctcacag gtgaacccct ttccgggccg cgtttcacgg
//
LOCUS       DAOPTR010000198         4320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16414_length_4320_cov_3.339273, whole genome shotgun sequence.
ACCESSION   DAOPTR010000198 DAOPTR010000000
VERSION     DAOPTR010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16414_length_4320_cov_3.339273"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /gene="aat"
                     /locus_tag="PKI05_05115"
     CDS             complement(<1..535)
                     /gene="aat"
                     /locus_tag="PKI05_05115"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOA51614.1"
                     /translation="MSAFGSSVLPPSRFFPSPEWADEDGIVAVGGTLHPDLLLDAYSH
                     GIFPWPVDEESPIVWASPEIRAIFDWDKIHIPRRLVPTIKRDGFKVTLDQDFRSVIRG
                     CATAPGRRGNTWITPAMIRAYIRFHELGFAHSVEVWQGEKLVGGVYGVALGGLFAAES
                     MFYRVSNASKVGLLYLLA"
     gene            972..2834
                     /locus_tag="PKI05_05120"
     CDS             972..2834
                     /locus_tag="PKI05_05120"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOA51615.1"
                     /translation="MLNLVSSCDPKAGSFWACTLACLVFGISSYFVASSAAETKVGNV
                     LEETVLLDFTASWCGPCQRMAPLVDQLIGMGYPVRKVDIDREPALAKRFGIQAVPTFV
                     MLVNGREAKRIVGGAGLDDLIALCRLAPRAGSPSDSQSVVLTSATSSGVSPASTGVFP
                     LRGAALSDKAGARPATIPETIPPLDENCRFAAANEQDLIAVTARIRVEDPHGRSTGSG
                     TIIDARGGEALIVTCGHLFRDSHGKTPILVDLFGPNAAQGIPAQLIYYDDRLDIGFIS
                     IRPNRPVVVARVAAPGYPLAVGDAVFSVGCTASQPPSVWEGRITALNKYLGPANIEAS
                     RAPFVGRSGGGLFSNEGFLIGVCNAADPPLDEGIYLALQEIHQSLDQLNLAFVYRWDA
                     ASRGMTGDPQVAQNRGQLAVSGPGGGSSGRGETENRFGQPQGPSTGLSPESLTLATGL
                     PLPIPMGDLSGSRTAQAMGTLGVPVPSGGMPSATVVPVGFVEHATLSLEEQATLEEIR
                     RRQAQGHQVVCIVQPADQPNSTSEIIVVRSPSPQLLAALSQAGVKMVMPGDLPSRTVG
                     EIPSDPFSARLGQQASDSPLVPIPAGAVLRIGTNVMPVSGRAVAGTRPGTAGWD"
     gene            3245..3535
                     /gene="gatC"
                     /locus_tag="PKI05_05125"
     CDS             3245..3535
                     /gene="gatC"
                     /locus_tag="PKI05_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOA51616.1"
                     /translation="MISLAEIEKVAVLARLLLTPAESQQMTEQLGKILEYMSLLNEVN
                     TENVEPLAHAVELTNVFREDRVTPSLDREMALANAPHRDEECYRVPAVLGEV"
     gene            3590..>4320
                     /locus_tag="PKI05_05130"
     CDS             3590..>4320
                     /locus_tag="PKI05_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOA51617.1"
                     /translation="MSLSELTASQLLQQLREGRIKAVEVVAHSLRQIDQYDPTVGAFL
                     RVNRDHAFAQAEEIDRRRSRGEKLGKLAGLPVAVKDNICTQGEPTTCASRMLKRFVPP
                     YDATVIAKLKAADAIIVGKTNLDEFAMGGSTETSALGRTCNPWDVTRIPGGSSGGAAA
                     CVAARMVPLSVGSDTGGSIRQPAALCGVTGLKPTYGRVSRFGLVAFASSLDQIGQLAQ
                     TAEDCALLLEVLAGHDPKDATSIPQP"
BASE COUNT          850 a         1200 c         1347 g          923 t
ORIGIN      
        1 gggcgagcag atagagcaac cccaccttgg aggcattact gacccgatag aacatcgact
       61 cggcggcaaa taacccccca agagcgactc cataaacccc accaacaagc ttttcgccct
      121 gccagacctc aacgctatgg gcaaagccta actcgtgaaa acggatatag gcccttatca
      181 ttgcgggcgt gatccatgtg ttgcccctcc gaccgggggc ggtggcgcaa ccccgaatca
      241 cgctgcgaaa gtcctgatcg agggtgactt taaaaccgtc cctcttgatg gtcggcacta
      301 atctcctcgg aatatggatt ttatcccagt caaaaatggc tctgatctcg ggagatgccc
      361 atacaatggg ggattcttca tccaccggcc atgggaatat cccatgagaa taggcgtcca
      421 gcagcagatc ggggtggagg gtgccgccga cggcaacgat accgtcctcg tccgcccatt
      481 caggcgatgg aaaaaatcgg gacggcggca ggacggagga accaaacgcg gacataatcc
      541 cctctgtggg tggtatctat gggcgttgcg cgagcgacgt gcgccgctaa attggcattg
      601 cccaattcta tcgactggtt tttaatgggt gaaccggttg cgcgggaatg gcacaaaaag
      661 ctggccgcca attggtctgc gatctactgc cccgagacaa acgacctttg gtagtgtaaa
      721 agagtacccc tatttgatcg ggaacatgga ggtccccgga ttgcaggtct ttgacaagga
      781 cgttgaaaca tccctggagt tcgttcgtga ccgggctttt ccgttttcta cggtaggcac
      841 ggtatcaact tcgggcagat cactgcgtct tttccctagg acgtttggcg ctgccctatc
      901 cgtaaacttg ccctttaatc gcatggtgga atggattcct tcgtcaacgc ccaattcagg
      961 aggaaagggc catgctgaat ctggtttctt cttgtgatcc caaggcgggg agcttctggg
     1021 cttgtaccct ggcatgcctt gtcttcggca taagttcata tttcgtggcg tcatctgccg
     1081 ccgagacgaa agttggtaac gtcctggaag agacggttct tttggacttc acggcgagtt
     1141 ggtgcggacc ctgccagcgc atggcgcccc tagtcgatca actgatcggc atgggttacc
     1201 ccgttcgcaa ggtggatatc gaccgggagc cggccttggc caaacgtttt ggaatccagg
     1261 ccgtgcccac atttgtgatg ctcgtcaatg gtcgggaagc gaagcgaatt gtgggcggtg
     1321 cgggcctgga cgacttaatt gccctatgtc ggctggcacc gcgggcaggt tcgccgtcgg
     1381 attcgcagtc ggtcgttctt acctcggcga caagctccgg tgtctctccg gcatcgacgg
     1441 gagtgtttcc cctgcgcggt gcagcgcttt ccgacaaggc aggggcaagg ccagcgacca
     1501 tcccggaaac catccccccg ctggatgaaa actgtcgctt tgccgctgcc aatgagcagg
     1561 acctcattgc ggtaaccgcg cggattcgcg tggaagatcc ccatggccga tcgaccggct
     1621 ccgggacgat catcgacgca cgcggcggcg aggcgttgat tgtcacgtgt gggcatcttt
     1681 tccgtgacag ccatggaaaa acacccatcc tcgtggacct ttttggcccg aatgccgccc
     1741 aaggaattcc ggctcaattg atctactacg atgaccgttt agatatcggc ttcatcagca
     1801 ttcgcccgaa ccgccccgtg gtggtggcgc gggtagcggc gcccggatac ccattagcgg
     1861 tgggcgacgc cgtcttctct gtaggatgca ccgccagtca gccgcccagc gtgtgggaag
     1921 ggcgtatcac ggccttgaac aagtacctag ggccggccaa tattgaggcg tcgcgggcac
     1981 cgtttgtcgg cagaagtggc gggggactgt tttccaacga aggctttctc atcggtgtgt
     2041 gcaatgccgc cgatccgcca cttgatgaag gcatttattt ggccctccaa gagatccacc
     2101 aatcgctcga tcagctcaat ttggcctttg tctatcgctg ggacgctgct tcccgcggaa
     2161 tgaccggaga ccctcaggtt gcccaaaatc gcggacaact ggcggtgtct ggacccggcg
     2221 gagggtcctc agggcgtggt gaaaccgaaa atcgttttgg ccagccacaa ggtcctagca
     2281 cgggcctatc gccagagagc ttgaccctgg ccaccggttt gccgctgccc atcccgatgg
     2341 gtgatttaag cggctcgcgc accgcgcagg cgatgggcac attgggagtt cccgtgccaa
     2401 gtggcggaat gccatccgca actgtggttc ctgtgggatt tgtggagcac gctacacttt
     2461 ccctggagga gcaggcgaca ctggaggaaa tcaggcgaag acaggcccag gggcaccagg
     2521 tggtttgcat cgttcaaccg gcggatcagc ccaactctac gagtgaaatc atcgtcgtgc
     2581 gaagtccgtc gccgcaactt ttggcagccc tgagccaggc cggggtgaaa atggttatgc
     2641 cgggcgattt gccgagccgg acggtgggcg agattccttc ggacccattc tctgcccggt
     2701 tgggacagca ggctagtgac agcccgcttg tacccattcc cgccggtgcc gtcctgcgga
     2761 tcggtacaaa cgtcatgccc gtttcaggac gagcggtggc aggaactcgg ccaggcacag
     2821 cggggtggga ctagggcacg caccgtgatg gcgattcggg cgggatggag cgacgattcc
     2881 gacgggtgcg acgacggcgg ttcggtcagg cacgccgacg gttattcggg cagtcacgtg
     2941 gggcggggat tgcggtaggg gcaccccccc gtggttgccc caaaaagttc aggcgggaac
     3001 gggggcggcg attcgggtgg gggcattctg gcgggtacgg cgatcgcaga tcagggcagg
     3061 cacgggggcc tgcccctacg aggggtggtt ttttcacatt cgggaacgcc gtaccgctca
     3121 tcggcgcgtt atactatctt ttttggcccg cggtggcctt gcctaaacgt tgagcctaaa
     3181 tggcttgttt gccttgccgg cggatgagcg ctgtccaacg tttatgtttt aggattcttc
     3241 caacatgatc agccttgcag aaatcgaaaa ggttgccgtt ttagcccggc tgttattgac
     3301 cccagccgaa tcccaacaga tgaccgagca attgggaaag atcctggaat acatgtcgct
     3361 tttgaatgag gtcaacaccg agaacgtgga accgttggca cacgcggtgg aattgacgaa
     3421 tgtgtttcgg gaagaccgag tcacccccag cctcgatcgc gagatggcct tggcaaatgc
     3481 accccatcgc gatgaggagt gttatcgcgt ccccgccgtt ttgggtgaag tgtgatactg
     3541 gggcatcctt gggtcaagct ctatgggact ttgaggaaga atgtgcgata tgtcgctttc
     3601 tgaattgaca gccagtcagt tgctccagca gctccgcgag gggcgaatca aggccgtcga
     3661 ggtggttgcc cattcgctgc ggcagattga tcaatacgat ccgacggttg gggctttttt
     3721 gagagtcaac cgggatcatg cgttcgccca ggcggaagag attgatcggc ggcggtcgcg
     3781 gggggagaag ttgggtaagt tggccggttt gccggtggcg gtcaaggata atatttgcac
     3841 tcagggagag ccaacgactt gtgcctcgcg gatgctgaaa cgttttgtgc cgccatatga
     3901 tgccacggtt attgccaagc tcaaggcggc cgatgccatc atcgtgggca agacgaatct
     3961 ggacgagttt gcgatgggcg gctcgacgga gacctcggcc ttgggccgca cgtgtaatcc
     4021 ttgggatgtc acgcggatac ccggcggctc gagtggtggg gctgctgcct gcgtcgccgc
     4081 ccgcatggta cctctgtctg tgggaagcga tacaggtggc tcgatccggc aaccggcagc
     4141 cctctgtggc gtcaccggcc taaagccgac atacggtcgc gtcagccggt tcggactggt
     4201 ggcctttgcc agtagccttg atcagattgg acagcttgcc caaaccgccg aggactgcgc
     4261 cctgctgttg gaagtcttgg ccggtcacga ccccaaggat gccacgtcta ttccccagcc
//
LOCUS       DAOPTR010000199         4319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16419_length_4319_cov_3.681754, whole genome shotgun sequence.
ACCESSION   DAOPTR010000199 DAOPTR010000000
VERSION     DAOPTR010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16419_length_4319_cov_3.681754"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PKI05_05135"
     CDS             complement(<1..656)
                     /locus_tag="PKI05_05135"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HOA51618.1"
                     /translation="MTIAYFDCFSGISGDMVLGALVDAGLKLEDLQHVVSTLGIPNCR
                     VNAQTVRKGGFRATQVVVEAPEEHHHRHLHHILEMVERADLTPRQKEWAEKVFVRLAE
                     AEARVHGIPIEKVHFHEVGAADSIVDVVGSVVGLDLLGIQEVYASPVPTGAGTVKIAH
                     GEVAVPAPATAELLRGVPLLPCDVMMEMTTPTGAAILTTFAKRFGPLPAMQIEQIGYG
                     A"
     gene            complement(1130..2248)
                     /gene="tcmP"
                     /locus_tag="PKI05_05140"
     CDS             complement(1130..2248)
                     /gene="tcmP"
                     /locus_tag="PKI05_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HOA51619.1"
                     /translation="MDPCPSYWLEYSNLQHTKHQLIREYLNGWFPKLGYSHSRVVYVD
                     THAGRGRHLQGQLGSPLVALQTLLQHKDRPRLLERCEVVFFFIERDETNFCELRSEIK
                     QLGNLPAKIVVNTSCKDCFKVLHDVVTSLRHAGQEMAPAFVFVDPYGFKIPGRVLREL
                     MEFPRVELFVNIIWRELDMAIRQERLRTKRSGMADTLDSIFDGSEWKDRVSSDDFDER
                     ANQAINLLREKIGAKWVTHIRMLGDNDVTRYLLMHLTNHDAGRDLMKACMWKVCPDGG
                     FYVRKTDDPAQQFLITPEPDLGPLAEWLRCRLSAGPRRWKELIAEVREQVWLPTHLNK
                     VIRELRRKKELVADDYRGRFDPINNPRLSLRVGEARGR"
     gene            complement(2257..3006)
                     /locus_tag="PKI05_05145"
     CDS             complement(2257..3006)
                     /locus_tag="PKI05_05145"
                     /inference="COORDINATES: protein motif:HMM:NF019146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HOA51620.1"
                     /translation="MGANTTISWTKRTWNPWRGCTKISPGCKNCYMFAAQLRYGNEPH
                     TVVRTRTWNDPLRWQRQAAYQGKWEFVFTCSWSDWFHEAADEWRGEAWQIIRRCPNLI
                     FQILTKRPERIADHLPDDWGEGYANVWLGTSIELNEYVWRADVLRKIPAVIRFISAEP
                     LLGPLTDLDLEGIHWLIVGGESGARYRPMKLAWVRELRDRALAQEVAFFFKQSAGRRP
                     ETGTLLDGRHWRQYPRRVSSVPVVSVGKGPR"
     gene            3823..>4319
                     /locus_tag="PKI05_05150"
     CDS             3823..>4319
                     /locus_tag="PKI05_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOA51621.1"
                     /translation="MKMTPRYLVPMLFLLPWLGCSRPERGTEPQEQSSSSGASAEQTL
                     PAAEIRVGDQTDLARIIEENRGKVVLVDFWATWCPPCMELLPHTLEMAKKYASRGLTV
                     ILVAINDPDEQADDVKAVLERNQAAGVVNLISRYGIGLEAVEKFEIDDGALPHLRLYD
                     RQGRL"
BASE COUNT         1019 a         1196 c         1114 g          990 t
ORIGIN      
        1 gccccgtaac cgatctgctc gatctgcatg gcgggaaggg gaccaaaccg cttggcaaag
       61 gtcgtaagga tcgctgcccc ggtcggcgtc gtcatttcca tcatgacatc gcaaggaaga
      121 agcggcacac cgcggagtaa ttcggcagtc gccggggcgg gcacggccac ctcaccatgg
      181 gcaatcttca ccgtcccagc accggtcggc accggcgagg cgtaaacctc ctggatgccc
      241 agcaggtcga gcccgaccac gctaccgacc acatcaacaa tcgaatcggc agcaccgact
      301 tcgtgaaaat ggaccttttc gatgggaatg ccatgcacac gggcctcggc ttctgccaag
      361 cggacaaaaa ccttttccgc ccattccttt tggcgaggcg tcaaatcggc acgttccacc
      421 atctccaaga tgtgatggag atgccgatgg tggtgctcct cgggggcctc gaccacaacc
      481 tgcgtcgcgc gaaagccccc ttttcgcacg gtctgggcat taacgcggca attgggaatg
      541 cccaacgtgg atacgacgtg ctgaaggtcc tccagcttga gcccggcatc caccaaggcc
      601 cccaacacca tatccccact gatgccgcta aaacaatcga aataggcgat agtcatagaa
      661 ggatgctttc tcgtcgtggg tcagctcagg caacaaaaac aatcacgatg gtaatcgcgt
      721 caaaagtcgc ggtgcagtca agagtttctg acgtttttgg actccgatcc gtctccgctg
      781 attctaaaca tccctgccgt tgtcgcaagt gcagtgcgcc ataccgcccc gatgtactgg
      841 acttcaccca gcgttgatct tgacatggca cggaaaataa ggactccctt cccttgaagc
      901 tggggagatt tcgggaaaag aggtgcaaca atggcaagga atggacatgg ttaaactctc
      961 cgtcgtacat aatgcttctc tgcattcctt ctacgagctc tttagccttc ctagctgcca
     1021 gaatccttgt cgtttcctat gcgtttcgac gacctctttg caaaccacga tgcaagaatg
     1081 ggtcacggcg tggttgtccc tcggtttgct gcttcttcgt tttcgaggtc tagcggccac
     1141 gggcttcgcc gactctcaga ctaagacgag gattgtttat cggatcaaac ctaccccggt
     1201 aatcgtcggc aacgagttct ttctttctgc gaagttcccg aatgacctta ttgagatgtg
     1261 taggcagcca gacttgctcg cgaacctcgg cgattagctc tttccaacgt ctcgggcccg
     1321 ctgacagcct acaacgcagc cattctgcca gcggccccaa gtctggctcg ggagtgatca
     1381 ggaattgttg tgccggatca tccgtctttc tcacgtaaaa tccaccgtct ggacacactt
     1441 tccacatgca cgccttcata aggtcgcgtc ctgcgtcatg gtttgtaaga tgcatgagca
     1501 gataacgcgt gacatcgtta tcgcccaaca tgcgaatatg tgttacccat tttgcgccaa
     1561 tcttttcacg cagcagattg atagcttgat tggcacgctc atcaaagtca tctgatgaca
     1621 cacgatcctt ccattcgctt ccatcaaaaa ttgaatctag ggtgtccgcc atcccactcc
     1681 tcttggtcct gagcctctct tgccggatag ccatgtcgag ttcacgccaa atgatgttga
     1741 caaagagctc gactcgcggg aattccatca actcgcgaag gacacggccc ggaattttga
     1801 agccataagg atctacgaag acaaatgcgg gagccatttc ctgtccagcg tgccgcaggg
     1861 acgtcacgac gtcatgcaaa accttgaaac agtccttgca ggacgtgttg accactattt
     1921 tggccggaag gttgccgagc tgtttgattt cgctccgcag ttcacagaaa ttcgtttcgt
     1981 cacgctcgat gaaaaagaac acgacttcgc atcgttccaa gaggcgaggg cgatccttgt
     2041 gttgaagcag agtctggagg gccacaagcg gcgaaccaag ctgcccttgc agatgccgcc
     2101 cgcgccctgc gtgagtgtcg acatacacta cacgactatg cgagtagcct aacttcggga
     2161 accacccatt gagatactcc cgaattaatt ggtgcttcgt gtgttgaagg tttgaatact
     2221 ctaaccaata ggaaggacat ggatccattg tcgacatcat cttggcccct tgcccacaga
     2281 cacaactggt acagaggaca cacgcctggg atactgacgc cagtgccgtc cgtcaagcaa
     2341 ggtccctgtc tcgggtcgcc tgcccgcact ctgcttaaag aagaatgcga cttcttgggc
     2401 caacgcacgg tctctcagct cgcgcaccca tgcaagtttc atgggacgat aacgggcgcc
     2461 agattcacct cccacgatca gccagtgaat gccctccagg tcaagatcgg tcaaaggtcc
     2521 caggagtggc tccgcggata tgaagcgaat aacggcggga atctttctga gcacgtccgc
     2581 acgccaaacg tactcgttta gttcgatact ggtacccagc catacgtttg cataaccttc
     2641 gccccagtcg tcgggaagat gatctgcaat tctttctgga cgcttcgtga gtatctggaa
     2701 tatcaagttc gggcaccgtc gaattatctg ccatgcttcg ccacgccact cgtcagcagc
     2761 ctcatgaaac caatcggacc aagaacacgt gaacacgaat tcccattttc cctgataggc
     2821 cgcctgtcgt tgccagcgca gaggatcatt ccacgttctc gtgcgaacga ctgtgtgcgg
     2881 ctcattgcca tagcgcaatt gtgctgcgaa catgtagcaa tttttacagc cggggctgat
     2941 cttggtgcaa ccccgccatg gattccacgt gcgtttagtc cacgaaatcg tcgtgttggc
     3001 acccatggtt caccgtccgt ctaaatcgaa gttcttgcgg tgctgatgtt cttctccaat
     3061 acatgtgaaa aatttttcgc ctgctatgac tgaattggcc gcacgcaaaa acctaatcac
     3121 cctcacagct aatgatagcg tacaagagta catcacaacg gatcaagggc gtcaaggcaa
     3181 tccgttggtg cgtggaattc actcatttcc ggttttgact gagaaagtcg gccttccttt
     3241 gtgtacagga agatgaaatc ttagaaggag agggccatgt gtgctgcctg cgtaggtcaa
     3301 atgaagatag gctatggact gtataacaag ggccattgaa ggttatatgc cttaaggtcc
     3361 ccaagattgg tgcctgatag gcggcaccag cgatttctcc aaaagatgtt ggcgattggg
     3421 cgctacgcga acctgaccga gatcacctgg tggtcagctt gatcgataca catggttgtg
     3481 cgggcgtttc gccgccgttt gagcgcagaa aatgtcactt agggtatcaa atgccacccc
     3541 gacgcttgta aaaactggtt actgatttgc tcgagtggcc ggccatccac atcagctaat
     3601 ctctggactc aaaacctaaa tgctgggctt gtgggggttt taacattagg cgaaatcgcg
     3661 agaacatgta tccttgaatt ccgcgaggac gatggggaat aataacggaa aggtgtacgg
     3721 tctgcctcga ttgcccgcgt catcaccgtc gccttcagag cggatcaaat cgccagccgt
     3781 cagaatatta ctttgcgaaa aatcacaaca gaggacctgc gaatgaaaat gacgccacga
     3841 tatcttgttc ccatgctctt cttattacca tggctcggat gcagccgccc ggaacgaggt
     3901 acggaacccc aagaacagag ttcatcttct ggtgcttccg cggaacaaac cttgccagcc
     3961 gcggaaatcc gggtgggtga ccaaacggat ttggcccgga tcatagagga aaatcgcggc
     4021 aaggtggtcc ttgtggactt ttgggcaaca tggtgtccgc cgtgtatgga gctcttacct
     4081 catacgctgg aaatggccaa aaagtatgca tcgcgaggcc tgaccgtgat ccttgtggcg
     4141 atcaacgatc cggacgagca ggccgacgat gtcaaagccg tgttggaaag gaatcaagca
     4201 gcgggcgtcg taaacctgat tagccgctat gggatcggtt tggaagccgt ggaaaaattt
     4261 gagatcgacg acggcgcact gccccatctt cggttgtacg accgccaggg gcgattgtg
//
LOCUS       DAOPTR010000200         4318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16423_length_4318_cov_4.081398, whole genome shotgun sequence.
ACCESSION   DAOPTR010000200 DAOPTR010000000
VERSION     DAOPTR010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16423_length_4318_cov_4.081398"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PKI05_05155"
     CDS             complement(<1..325)
                     /locus_tag="PKI05_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51622.1"
                     /translation="MNSLATVGLVIIAALIPLAVVWAESASAASSEGGKVFKAGAAAS
                     NITPWLDEGIVGGWSSPPGKYIHDELYARCLVLDDGSTQLAIVIVDNLGLAREVCDRA
                     KQSIRE"
     gene            complement(665..3472)
                     /locus_tag="PKI05_05160"
     CDS             complement(665..3472)
                     /locus_tag="PKI05_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA51623.1"
                     /translation="MKTLLLAAALVWGTLFTVGWAVAQMPKQPPNVIILITDDQGFGD
                     LSCHGNPVLKTPHLDRLHDESIRFTDFHVAPMCTPTRGQLMTGRDALRNGAMNVSSGR
                     TPLRREFPTMAEVFAASGYSTGLFGKWHLGDNYPYRPQDRGFQESIWYPSSHIGSVPD
                     AWENDYFDDIYFHNGRPRQFRGYTTDVFFTEAIRWMEEQAKQGRPFFCYLATAAPHAP
                     HFVPEQYRNAIRQSLEAARPQLSKFAAGLQPEARSSFEEELVRFLAMIANIDENVGRL
                     EGFLKSSGLRENTLLIFLTDNGSTFGPRYFNAGMKGGKTTLWEGGHRVPCFIRWPGGG
                     LRPAGDVPGLTQVQDIFPTLVELLQLRLPAGVQFDGISLAPVLRGQSEVPEDRMLVIN
                     YSRMPFKSTRTTADNNAVPRREGAAVLWKRWRLLEDKALYNLAHDPLQERNVIDQYPE
                     VAAKMRSHLNAWWDGVKARVNEFQAVIVGDDAENPTMLTACEWADVFLDQQAQVRRGE
                     RKSGLWHIEVAKPGEYAFMLSRWPYASGLRLRDGISETKVTDGILPAGEAWPVTAARI
                     RVGHMERVIRPVGDAIAARFVIPLDVGRTTLETMFLDENGQEIAGAYYVTVERLRQVE
                     QPAAVILDTDMSGDCDDAGALALLHALADRGECKILATIVNRKDKTNASAAAVDAINT
                     YYGRGNLPIGTDKRGPTDLQRTSAYAPALRDEFPSDIGPDDQAPDAVDIYRQILVSQP
                     DGSVTICSVGALSNLAELWRSEPELVRKKVRRLVIMGGEFPTSKTPETNIRTHREAAK
                     IVAEEWPGEIIWVGFEIGHIVFTGEGLKLTPRHNPVRRAYELRPFGGRPAIEGGQPSY
                     DQTAALIAVRGLEPDLWEVVTGGRVDVDAEGRTTWRVDPTGKHSYVRLACPPAKLATI
                     IENLMMAPPVAHMAEENDPRQ"
     gene            complement(3603..>4318)
                     /locus_tag="PKI05_05165"
     CDS             complement(3603..>4318)
                     /locus_tag="PKI05_05165"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF013461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOA51624.1"
                     /translation="LEAYYACLKRETFLENVWIEPDGTIDFFNMERTSNGRAVVERRD
                     FMHAAQSINSERVDNLFLITRGSTIPAIAKLTHEEAAAFMVLGQSMESSAGDPTQAGK
                     IKNCFFYDPFLAGDRAEHANLFYDILKANPHIQCYLLNTGYVGEGESMRDIRLADTMG
                     ILDSVLRGGLDEWVYSPRTKLYVPSAVRLVDSILFHPEKLFPHDVFEEKQKALEKHRA
                     EFLDRFPGLHPNIRKVFQR"
BASE COUNT          937 a         1311 c         1153 g          917 t
ORIGIN      
        1 gctcgcgaat cgattgcttt gcccgatcgc atacttcccg ggcaagcccc agattatcaa
       61 caatgacaat cgctaattgc gtgctgccat catcgagaac caggcaccgg gcgtacagct
      121 catcgtggat atacttgcct ggcggcgatg accatccccc gacgatgccc tcatctaacc
      181 agggcgtgat attactggcc gcggcacctg ccttgaaaac cttcccccct tccgaggacg
      241 ccgcagacgc tgactccgcc cagacaacgg ccagtggaat caacgctgcg atgatgacca
      301 ggccgacggt tgccaatgag ttcactactt ttcgcgtgtc tttcatcaac gtacggcggc
      361 gggctctgtg catactgcga atcatgttac cccccatgtt ctctaggtca cctcaaagac
      421 cacgttagag ttttcccaat ttccgctggt gcattatata gattcttgat caccgacgca
      481 tggtctccgc aagtttagcg gcttgtcacg ccgggttgac tgtttggtga ttttgctcag
      541 ggcgctcctc attatgtcgt cggtgattca aaacccggca accgccagtg gcaacatttc
      601 gccaccacac taccaaggca gtggctcata tcagcggtgg aggcgaggca tacgtcggcc
      661 ctgcctattg acgtgggtcg ttctcttcag ccatgtgggc aacgggggga gccatcatga
      721 gattttcaat gatggtagcc agttttgcag gcgggcatgc caagcggaca tagctgtgct
      781 tgcccgtcgg atcaactcgc cacgttgttc ggccctccgc atccacgtcc actcggccac
      841 ctgtgaccac ctcccacaaa tccggctcca ggccacggac ggcaatcaag gccgctgttt
      901 gatcataact gggctggcca ccttcgatgg caggccgtcc tccaaacggc cgcagttcgt
      961 aggcccggcg aacgggattg tggcgcggcg ttaatttgag tccttctccg gtgaagacga
     1021 tatgaccaat ttcaaatccc acccagatga tctcacccgg ccactcctcg gcaacaattt
     1081 tggccgcctc gcggtgagta cggatgttcg tctcgggcgt cttcgaggtg ggaaactcac
     1141 cacccatgat caccaaccgc cgaactttct tgcggaccaa ctccggctcg ctgcgccaga
     1201 gctccgctag gttggacaat gcccccacgc tgcaaatcgt aacacttccg tctggttgag
     1261 aaacaagaat ctgtctgtaa atatccacgg catcaggcgc ctggtcatca gggccaatgt
     1321 cgctggggaa ttcatctcgc aaagcgggcg cgtaagcact cgtcctttgc aggtccgtgg
     1381 gaccccgttt gtcggtgcca atcggtagat ttccgcgacc gtagtaggtg ttgatcgcat
     1441 ctactgcggc tgccgaggca ttggtcttgt ccttccgatt gacgatcgtt gccaaaatct
     1501 tgcattctcc gcgatcggcc agggcatgga gaagcgccaa ggccccggcg tcatcgcaat
     1561 ccccggacat gtccgtgtct aagataacag ccgctggctg ctccacttgc cgaagtcgtt
     1621 ccacagtgac gtaatacgcc ccggcaatct cctgaccgtt ttcatccaaa aacatcgttt
     1681 ccaaggttgt tcgaccgaca tccaagggaa tgacaaacct cgcggcaatg gcatcaccca
     1741 ccgggcgaat gactcgctcc atgtgaccca cgcgaatcct tgcggcggtc accggccaag
     1801 cctcgcccgc tggcaagata ccatcagtca ccttggtctc gctgatccca tcgcgtagcc
     1861 gcagccccga agcgtagggc caccggctta acatgaaggc atactcgccg ggtttggcca
     1921 cctcgatatg ccaaaggccg ctttttcgtt ccccccgtcg tacctgtgcc tgctggtcaa
     1981 gaaagacgtc ggcccattcg caggcggtca acatcgtggg attctccgcg tcatcaccca
     2041 caatcaccgc ctgaaattca tttacccgtg ccttcacgcc gtcccaccag gcattcaaat
     2101 gggatcgcat cttcgcggcg acctctggat attgatcaat cacattccgt tcttgaaggg
     2161 gatcgtgcgc taggttgtag agggccttat cttccagtaa ccgccaccgc ttccaaagca
     2221 cagcagcgcc ttcccggcgt gggacagcgt tgttgtcggc cgtcgtgcgg gtgctcttga
     2281 agggcattcg gctgtaattg atgaccagca tgcggtcctc tggcacttcg ctctgcccgc
     2341 gcaacaccgg cgccaaactg attccatcga attgaacgcc tgccggaaga cgcagttgta
     2401 acagctcgac gagcgtagga aagatatctt gaacctgggt caatcctggg acatcacctg
     2461 ctggacgtag cccaccgccc ggccaacgga tgaagcaagg cacacggtgc ccaccctccc
     2521 acaatgtggt cttgccccct ttcatgcccg cattaaaata acgcggtcca aatgtgctgc
     2581 cgttgtcggt caaaaagatg agcagggtgt tttcccgtag gccagagctt ttcagaaagc
     2641 cttccagacg gccaacattt tcatcaatat tggcaatcat ggccagaaag cggaccaact
     2701 cctcctcgaa cgagctacgt gcttccggtt gcaacccggc ggcgaacttc gaaagctgag
     2761 ggcgtgcggc ctcaagcgat tggcgaatcg cattacggta ctgttccgga acgaaatgag
     2821 gggcgtgggg tgccgcagtg gcaaggtaac agaagaaggg tcgcccctgc ttggcctgct
     2881 cttccatcca gcgaatggcc tcggtaaaaa acacatcggt cgtgtagccc cggaactgac
     2941 gcggacggcc attgtggaaa taaatgtcgt caaaatagtc gttctcccac gcatcgggaa
     3001 ctgagccgat gtgagaggac ggataccaga tgctctcttg aaacccgcgg tcctggggcc
     3061 gatacgggta attgtccccc aagtgccatt ttccaaagag gcccgtgcta taacccgagg
     3121 cagcaaagac ctctgccatg gtgggaaact cgcggcggag aggcgtgcgg ccgctgctca
     3181 cattcatggc cccgttgcga agagcatccc taccggtcat aagttgcccc cgcgtgggcg
     3241 tgcacatggg cgccacgtgg aaatccgtga agcggatgct ctcgtcatgc aaccgatcca
     3301 ggtgcggcgt cttcaagacg ggattgccgt gacatgaaag atcaccgaat ccctgatcgt
     3361 cggtgatgag aatgatgacg ttgggtggct gcttgggcat ttgggcgacg gcccaaccaa
     3421 cggtgaacaa cgtcccccac accaatgcgg ccgccagcaa gagagttttc acaaccatcc
     3481 tcctcgacct cggagtacgc caagttcatc ccgaaagcgc aaacgctccc gggaacccgc
     3541 cctgggtttt ccgcagtcta aggaggattc atcaaaatag caagcgccgc tggcccaacc
     3601 cgctaccgct gaaaaacctt acggatattc gggtggagtc cggggaaacg gtctaaaaac
     3661 tctgcccggt gcttttccag cgccttttgc ttttcctcaa agacatcgtg ggggaacaat
     3721 ttctcgggat ggaagaggat ggagtcaacc agcctcaccg ccgaagggac atatagcttg
     3781 gttcgcggcg agtacaccca ttcatcaagg ccaccccgca acacggaatc gaggatgccc
     3841 atcgtgtcgg ccaggcgaat gtcccgcatg gactcgcctt cgccaacata acccgtattc
     3901 agcaagtagc attgaatgtg cgggttcgcc ttgaggatgt cgtaaaacag gttggcatgc
     3961 tcggcccgat cacccgccaa aaagggatcg tagaagaagc aattcttgat cttgcccgcc
     4021 tgggttggat caccggctga agactccatc gactggccca ggaccatgaa ggctgccgcc
     4081 tcctcgtgcg tcaattttgc aatcgccggg atggtgctac cccgcgtgat gaggaacaaa
     4141 ttatcgaccc gctccgagtt aatggattga gcagcgtgca taaaatcgcg gcgttcgacc
     4201 accgcccgac cgttggaggt acgttccata ttgaaaaagt caatcgtccc gtcgggctcg
     4261 atccagacat tttccaggaa cgtttctcgt ttcaaacagg catagtaggc ttcgagct
//
LOCUS       DAOPTR010000201         4309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16465_length_4309_cov_2.849318, whole genome shotgun sequence.
ACCESSION   DAOPTR010000201 DAOPTR010000000
VERSION     DAOPTR010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16465_length_4309_cov_2.849318"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PKI05_05170"
     CDS             <1..346
                     /locus_tag="PKI05_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51625.1"
                     /translation="IDMIPKDIVICDWHYGKHQEYPSVRYFQEKGFRVWPAGWNNVDA
                     VRSLIRYSRQNNMGGVVGYLATTWVLEPGGFCWALVGERHPALVSQRALDAAQAFHAA
                     MEELNASADQPH"
     gene            486..1952
                     /locus_tag="PKI05_05175"
     CDS             486..1952
                     /locus_tag="PKI05_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOA51626.1"
                     /translation="MRTRIAIYWLVVVFGFVGVAQQTGTVIFAEESSVRAKNVILMVA
                     DGAGFNTWLATSMYRGRVGREIYDQPGWVRLACTTFPLNTATQPKKTGQQDPNVVYSP
                     SKAWDAESGYKWLTSSATDSAAAATALSTGQKSYNNAINWSDMDQAMMGESLPEVAYR
                     AGKSAGVVTSVQWSHATPAALGGAHNRDRDNYAEIAQEMLAAPYLQVIMGAGHPLFDK
                     NGQPKKGKLEEADYRYVGGRQEWEKLTSGQHPGGWTLIERKEDFEKLTTGPAPKKVLG
                     TAQVATTLQQARGGLIKVVLPGEPFAEPLNDNVPSLAVMVEGALNVLDNDPDGFFLMV
                     EGGAVDWANHANQLHRMIEEQSDFLAAVEVVVNWVNKNSSWDETLVVLTADHECGCLW
                     GPDSDKKPFAPIQDAGAGKMPAARYNATKHTNSLVPLYARGVGAERFAAFVRGEDAQA
                     AQVWGISGKYVDNTDVGRILRSVILGQPVAVHHHVTMP"
     gene            2177..2785
                     /locus_tag="PKI05_05180"
     CDS             2177..2785
                     /locus_tag="PKI05_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51627.1"
                     /translation="MGFHFFCPQGHILEAEIEQVGQAAVCPFCGTEMLIPAPLNQPTA
                     ASSQEDSRQASVNVPTESALLGHGQEDAIGGEVRGSPSFASVSGLSVEPGAFELSAGP
                     EPAIGVSLGAEESQSVFHIPCPNGHILETPADMLGRPVMCPICQTTFKPQYEASEEYR
                     RKREEMAAAAAARAARFWLQLAIGAAIIVLGGLIMLIVFAPR"
     gene            2792..3160
                     /locus_tag="PKI05_05185"
     CDS             2792..3160
                     /locus_tag="PKI05_05185"
                     /inference="COORDINATES: protein motif:HMM:NF016178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HOA51628.1"
                     /translation="MLLFKKRFLDDIRTGRKTQTIRVWKVRHMRAGQLSYIPGVGYIR
                     VDSVDEVALNDLTDEDAIPDGFLSVQELRAEIERLYSCDQLCAHRVFRIRFHVLPSDE
                     QRKIREQRRARREGTRRQDQ"
     gene            3585..4001
                     /locus_tag="PKI05_05190"
     CDS             3585..4001
                     /locus_tag="PKI05_05190"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HOA51629.1"
                     /translation="MGSRVRLSPAALYEAELNKRLQMSTAEIGLSVRTTNCLEENGIL
                     TVAQLLNTSPERLLSIANFGEKTLEEVFLALERLGFFRRSRLRSLGLEDRPTPFVPEM
                     SESSVIQFRIVFDDSTATPVFEENNETEKVADSVGS"
BASE COUNT          956 a         1074 c         1308 g          971 t
ORIGIN      
        1 tatcgacatg atccccaagg atatcgtcat ttgtgactgg cactatggaa aacaccagga
       61 atatccttcg gtccggtatt tccaggagaa gggctttcga gtttggccgg ccggatggaa
      121 taacgttgat gccgttcgat cactaatacg ctattcccgc caaaacaata tgggcggcgt
      181 cgttggttac ctcgcgacca catgggttct tgagcctggc ggcttctgtt gggcacttgt
      241 cggcgaacga caccctgcct tggtctcgca gcgtgccttg gatgccgccc aggcctttca
      301 tgccgcgatg gaagaactta acgcctcggc cgatcagcct cactagtcag tcagttggtt
      361 aatatttcgt taagacggta tttgccccgt tgatctgcga ggaatttcag ccagaattaa
      421 gtgtcttgac ctacaagaca gaagaatttt cttgcccaag cggtcttagc gaaaggagag
      481 tcttgatgag gacccgtatt gcaatctatt ggctggtagt cgtttttggt tttgtcggcg
      541 ttgcgcagca aactggtacg gttattttcg ccgaagaatc gtccgttcgc gccaaaaatg
      601 tgatcttaat ggtggccgac ggggcgggct tcaacacgtg gttggcaacg agtatgtacc
      661 gcgggcgtgt agggcgcgaa atctacgacc aaccggggtg ggttcggctg gcgtgtacga
      721 catttccttt gaacacggcg actcaaccga agaaaacggg tcaacaagac cccaatgtcg
      781 tgtactcgcc ttcgaaggcc tgggatgctg agtcgggcta caagtggctg acgagctccg
      841 ctacggattc agcggcggcc gcgacggcac tttccacagg ccaaaagagc tataataacg
      901 cgatcaattg gtccgatatg gaccaggcca tgatgggtga gtcgcttcct gaagtagcgt
      961 atcgggctgg gaaatcagcg ggcgtggtga cgagtgtgca gtggtcgcat gcgacaccgg
     1021 cagctttagg tggtgcccat aaccgagatc gggacaatta tgctgagatc gctcaggaaa
     1081 tgctggcggc tccctatttg caagtgatca tgggggcggg ccatcctttg tttgacaaaa
     1141 acggtcaacc aaaaaagggt aagttggaag aagcggatta tcgttatgtt ggagggcggc
     1201 aagaatggga aaaactgaca tcgggccagc acccaggtgg gtggacgctc attgaaagga
     1261 aagaagattt cgaaaaactc accacaggcc cagctcctaa gaaggtactg ggaacagccc
     1321 aggtggccac aactctccag caagcacgcg ggggcttgat caaagtggtc ttgcctgggg
     1381 aaccgttcgc tgagccatta aatgacaacg tgccaagcct ggcagtgatg gtcgaagggg
     1441 cactcaatgt gttggataat gatcccgatg gtttcttcct gatggtcgag ggcggcgctg
     1501 tggattgggc taatcacgcc aatcaactcc atcgcatgat cgaggaacaa agtgattttt
     1561 tggctgcggt cgaggttgtc gttaattggg tgaataagaa cagctcctgg gacgagacgt
     1621 tggttgtctt gacggcagat cacgaatgcg gctgtctctg ggggccggat tctgataaga
     1681 aaccgtttgc ccccatccaa gatgctggtg ctgggaagat gcccgcggct cgttataacg
     1741 caacgaaaca cacgaactcg ctggtaccgt tgtacgcccg cggagttggg gcagaaaggt
     1801 ttgccgcttt tgtgcgcggt gaagatgccc aggcggctca agtctgggga atctccggta
     1861 aatacgtgga caacacggat gtgggtagga ttctgcgatc tgtcatactc ggccagccgg
     1921 tcgcagtcca tcatcatgtg acaatgccat gacaagggaa accgttcttg ggcggcacac
     1981 gcataacaac ccagcggaca gcgtgatcgg accacagata tatgcgtggt tgcgtgcggt
     2041 tgttagaatg acacgtgggt gacgaccgaa gtattggtca gggatcgcaa aacttggtaa
     2101 cacagtggtg gtagcggagg cacttgcacg cggtgccatg ttccgccagt ggtgatgcgt
     2161 gacggacggg gacgtcatgg ggtttcattt cttttgcccc cagggtcata ttttggaggc
     2221 agaaatcgag caggtaggcc aggccgcggt ctgcccattt tgcggtactg aaatgcttat
     2281 ccccgcgcct ttgaatcagc caaccgccgc gtcatctcag gaggattccc gtcaggcgtc
     2341 tgtaaatgtt cctaccgaga gcgctctgct tgggcatgga caagaagatg ccattggcgg
     2401 cgaggtccgc ggttctccca gtttcgcttc tgtatcaggc ctctccgtcg aaccgggagc
     2461 atttgagctg tcggccggac cagagcctgc gataggtgtg tctctggggg cggaggaaag
     2521 tcagagtgtt ttccacatac cgtgtcccaa tggacatatt ctggaaacac ccgcggacat
     2581 gctcggcagg ccggttatgt gccccatatg tcagacgaca ttcaaaccac aatacgaggc
     2641 cagtgaggaa tatcggcgca agcgagaaga gatggccgcg gcagcggccg cccgtgctgc
     2701 acgtttttgg ctgcaactcg ccatcggggc ggccattatc gtacttggtg gtttaatcat
     2761 gctcatcgtt tttgctcccc gttgatgtga catgttgctg ttcaaaaaga gattcctgga
     2821 tgatatccgc acaggtcgta agacccagac aatccgcgtg tggaaggttc ggcacatgag
     2881 ggcggggcag ctcagttaca tccccggggt tgggtacatc cgcgtcgatt ccgtagatga
     2941 ggtggccctt aacgacctga cggatgaaga cgccattccc gatggctttt tatcagtcca
     3001 ggagctacgc gcggagatcg agaggcttta tagctgcgac caattatgcg cccatcgggt
     3061 cttccgcatt cgctttcacg ttttgccttc ggatgagcag cggaaaatca gagaacaacg
     3121 gagggcccgt cgcgaaggca cccggcgcca ggatcagtga gtgtccggcg ggttgacacg
     3181 gtctgaatcg gggaagtcaa tgtcccaaaa ccgtttcgaa taacgcgccg gctccttttg
     3241 ggacaaagcc ccctcacgga cgcgaggatg cccttattgg ggggaaggtc cccgccgatg
     3301 gccggcaagt cgggtctctg gataactttc gcaaactttg gcgctccgcg tattccctaa
     3361 aatcggcaca tttgctatat tcggtattgc tcgttgaccg agcgagaccc cttctgcgag
     3421 gctgggagga atgttagggg tcaggcaatt ccgttgcaaa ccgaggcgta gcgagtcgtc
     3481 ggtggtcggc cctcgcagaa ggataaggca gcgagtgagt gtcttgtatt ccttgaccgg
     3541 caacggatag ccatggcttt ttggtcgtcc aaggagggaa cgttatgggt tcacgcgttc
     3601 gcttaagccc agcagcactg tacgaggccg agctgaacaa gcggctgcag atgagcacgg
     3661 cagagatcgg cctgtcagtc cggacgacga attgtctcga ggagaacgga atcctcacgg
     3721 ttgcccagct tctcaatacc tcccccgagc gacttctcag tattgcgaac tttggcgaga
     3781 agacgctgga agaagtcttt ctcgccctgg agcgactggg attctttcgt cgcagccgtc
     3841 tccgtagttt aggcctggaa gatcgtccta caccttttgt ccccgagatg tctgagtcga
     3901 gcgtcatcca gttccggatc gtgttcgacg actcgacagc gacccccgtt tttgaggaaa
     3961 ataacgagac ggagaaggtt gccgactccg tagggtcatg atgaatcagt ggggaacacg
     4021 tcggagcttt cttgacggcc atcccgcaag caggctccca ctgtggctga aatcaaggac
     4081 aacgcgctgg ggagataggt ttctcctcaa aagaactacg gacgggaaga aatgtgaagt
     4141 agtttggcga aatcggtccg gcgggtcggc aacgacgctg aggctggaaa gacaccaatt
     4201 tggctcgctg gtttcgttta tttgaaaaga agcgcggtaa tcgtcgaacc ggatccgagc
     4261 agatcgccgg aatctgggag ggggcgtttt tcggcgtctt gttcctgtt
//
LOCUS       DAOPTR010000202         4306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16482_length_4306_cov_3.878617, whole genome shotgun sequence.
ACCESSION   DAOPTR010000202 DAOPTR010000000
VERSION     DAOPTR010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16482_length_4306_cov_3.878617"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..959
                     /locus_tag="PKI05_05195"
     CDS             144..959
                     /locus_tag="PKI05_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOA51630.1"
                     /translation="MAWPFLRIGNQTAFSAHHPDDPFRFAIEQGFDAFEWFNDKRHGW
                     GWCQEDVDDYDREQIRQLGEKHSIVFSVHARCQANINTPQGRDDVLRSIEFAKDIGAV
                     LVNVYYHGDQGPQGFAASLAPLVEAAAPANIRISVENTVFVSPHELNELFAALDATDW
                     CRPGAVGVCFDMGHANLCAATRYDYVGFLNQLESNVPIIHLHVHENWGDGDSHLPLFT
                     GPAAQDDSGVRTMLGILRERGYDGLIIMEQWPQPADQLITTRNRLRELLHAVS"
     gene            1155..2309
                     /locus_tag="PKI05_05200"
     CDS             1155..2309
                     /locus_tag="PKI05_05200"
                     /inference="COORDINATES: protein motif:HMM:NF017003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg1974 family pore-forming outer membrane
                     protein"
                     /protein_id="HOA51631.1"
                     /translation="MVCSSWLVKLHLRDAMNARMWLGSVAVCLLTASAALAQYGPYYG
                     AGTAPAWQGVMPTGYFEGNNGQALAPTPDQGTATTSLGPCPTGCLPTWQVWGEYLYLR
                     PSDADVPWAVAINGAIQQGSVPIQVSRVANADIDFDSGFRFGVGRALDECASVGVSYT
                     FFESHSNDSLATDDPYVLRSLVAHPGTLTAAADYLRGIASNDVDFQLIDADYRHVFRS
                     NCYGSMNWLVGARYAQFEQDFNALFRNNGIETVLTESRFYGAGLRLGLEGERHSQKCG
                     LLIYGRSTANFVAGDFRARYQQSQSYDVIEVDTRLRASRVVPILDLELGSGWVSQDGR
                     CMFTVGYMVSAWFNVLDADTFIRGVQANDFSDLDDTVVFDGLVGRAEIRF"
     gene            2773..>4306
                     /locus_tag="PKI05_05205"
     CDS             2773..>4306
                     /locus_tag="PKI05_05205"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOA51632.1"
                     /translation="MSHRRAFPCAIICGLVLGVMSIKSSSLAQDFYVSPEGNDRWSGR
                     LATPNAEKTDGPFASLARAQQAVRELRNQQPDRDRPIVVAIRGGRYFLSEPLRFGPED
                     SGTEKSPTIYRAHENERPIISGGVSLRGWTVDSQGRWHLMLDDVKAGKWNFTQLFVND
                     QRRYRPRLPKKGYYTIAERVPPPADNPVKGDDRLQYAEAEFNPQWSNLGDVEVNIFHQ
                     WSMSRMRIATIDASKKLVVFRRGTGFDSHWAHFLKGNRYYIENVADALTEPGEFYLDQ
                     KKGELIYIPRPGEDPQRADVIAPRLENLVIFACDGANKRWVEHLQFHGLIFAHSNWVC
                     PENGQFYPQAEVGLDGTISAIGARNVVFDACAVIHTGTYAMAFGEGCRNCQVENCELV
                     DLGGGGVKIGHAGSANWQGASQRPQGEEALVSHITVKNCTIAHGGRIHPAAVGVWIGH
                     SAYNTVEHNDIFDFYYTGVSVGWTWGYADSPAHHNAINYNHIYNIGQYVLSDMGGVYT
                     LGV"
BASE COUNT          877 a         1162 c         1268 g          999 t
ORIGIN      
        1 actggataca gtctgcgcgg gccggttttt tgaaacgtgg ctactgacaa ggcgagcgtg
       61 gtggcggttt attgcctttc tagtatccgc gtccttatac tggatgcgga ttgaggcagg
      121 cattaatccc cggaggacca cttatggcct ggccatttct gcgaatcggt aaccaaacgg
      181 cgttcagtgc ccatcacccc gacgatccct ttcgtttcgc gatcgagcag ggatttgacg
      241 cctttgaatg gttcaacgac aagcggcacg gctgggggtg gtgtcaggaa gacgtagacg
      301 attacgatcg cgagcaaatc aggcaattgg gcgagaaaca ctccatcgtc ttcagtgtcc
      361 atgcccgttg ccaggccaat atcaacacgc ctcaggggag ggatgacgtc cttcgcagca
      421 tcgaatttgc taaggacatt ggggcggtgc tggtgaatgt ctattatcat ggcgaccagg
      481 ggccccaggg ttttgcggcc tcgttggccc cattggtgga ggccgctgcc cccgctaata
      541 ttcgtatcag cgtggaaaat acggtcttcg ttagcccgca cgaattgaac gaactatttg
      601 ctgcactcga cgccaccgat tggtgccgtc ccggcgcggt gggggtttgt tttgatatgg
      661 ggcatgccaa tttatgtgcg gccacccggt acgactacgt gggttttctt aaccagttgg
      721 aatccaacgt gcccattatc cacctccatg tccacgaaaa ctggggagac ggggatagcc
      781 atctgccgct tttcactggg ccggcggctc aggacgactc cggcgtgcgg acgatgctgg
      841 gtattctgcg agaaaggggt tacgatggtc ttatcatcat ggaacaatgg ccccagccgg
      901 ccgatcaatt gattactacg cgcaaccgtt tgcgagagct tctccacgct gtgtcgtagg
      961 ctatttcgcg aaagattctc cccgctgagt gctaccccag tatggcatcc agacttcggc
     1021 tgagtgtaac gctgagacgg taaaataggg gttctacgca ggctattccg gtaaatcggc
     1081 tttttctggt cctggatgtt cgcaagaagc gatacactcc atacgagcag ccggatcagg
     1141 atggccggtt gtctatggtc tgtagcagtt ggttggtcaa attgcacttg agggatgcca
     1201 tgaacgcacg gatgtggcta ggcagtgtgg cggtttgcct gttgacggca agtgcggcgc
     1261 ttgcgcaata cgggccttac tatggagcgg ggacagcgcc agcttggcaa ggtgtgatgc
     1321 ccactgggta tttcgaggga aacaatggtc aggctttggc gccaacgccc gatcagggta
     1381 ctgcgacgac atcgttgggg ccttgtccca cagggtgttt gcccacgtgg caggtctggg
     1441 gagagtacct gtatctgcga ccaagcgatg ctgatgtccc ttgggcggtg gccattaacg
     1501 gggctattca gcaggggagt gttcccattc aagtgagtcg ggtggcaaat gccgacattg
     1561 actttgactc cggcttccgt ttcggtgttg gtcgcgccct tgacgagtgt gccagtgttg
     1621 gcgtcagcta cacattcttt gaaagtcact ccaacgattc cttagccacc gatgatccgt
     1681 acgtccttcg ctcgctggtg gcccatcccg gaacactgac agcggctgcc gattatctcc
     1741 gtggaatcgc cagcaacgac gtggacttcc agttgatcga tgctgactac cgccatgtgt
     1801 tccggagcaa ttgctacggc tccatgaact ggctggttgg tgcccgttac gcccagttcg
     1861 agcaggattt caacgcccta ttccgcaaca acgggattga aaccgttctc accgaaagca
     1921 ggttctacgg ggccgggttg cggttgggtc tggaaggcga acgccactcc cagaagtgcg
     1981 gcttgctgat ctatgggcga agcacggcca actttgtggc aggcgatttc cgtgctcgtt
     2041 accaacagag ccagtcctac gacgtcattg aggtggacac ccgtttgcgt gccagccgtg
     2101 ttgtccctat cctcgacctg gaattgggta gcggctgggt aagccaagat ggtcgttgca
     2161 tgttcactgt gggctacatg gtcagtgcct ggttcaacgt cctcgatgcc gacaccttca
     2221 ttcgcggcgt ccaggccaac gacttcagcg acctcgacga tacggttgtg tttgacggtc
     2281 ttgtgggtcg ggccgaaatc cggttctgat ctcgcccctg actggccaac gtagcacagc
     2341 acatccttca agtgcaatct cacccgccga gttttctcgg cgggttactt ttttcatgga
     2401 gcatctcttc gccaaaggca cggtggcgtg tgttcccggt tgttgtaact ctcggcgagg
     2461 cgcggtataa cctttcctta aattgacagt ctcaagatga atcggggact tagagcccat
     2521 cccataatct cccctcgccc gttcggtggg aaaggggtcg ggtgtgaggg tagctggagt
     2581 cgctgccggt tttctcgatc gactccgacg accgcttgcc ctcaccccaa ccctctcccg
     2641 gagggaaagg ggcttttgag ataggctctt gtgacgcggc gaacaccagg agagtcaaca
     2701 ccctttgacc ggagcaagaa gcttagcccg cctttttggc ccacatgaac gaggagcatc
     2761 gccgtgcgtt ttatgagcca tcgtcgtgcc ttcccttgcg caatcatttg cggtcttgtg
     2821 cttggggtga tgtccataaa gagctcatcg ctggcccagg atttttatgt gtcgccagag
     2881 ggaaatgatc gttggtcagg caggctggcc actcccaatg cggaaaaaac ggatgggccg
     2941 ttcgcctcgc ttgcgcgagc ccagcaagcg gtacgggaac ttcgcaatca acaacccgac
     3001 cgcgatcggc cgatcgtcgt agccatccgc ggtgggcggt atttcctcag cgagccacta
     3061 cgttttggcc ctgaagattc cggaacggag aagtcaccca cgatttatcg ggcacacgaa
     3121 aacgagaggc ctatcatttc gggtggagtc tcccttcgag gctggacagt ggacagccag
     3181 ggtcgttggc atttgatgct cgacgatgtc aaagcgggaa aatggaattt cacgcaactg
     3241 tttgtcaacg accaacggcg ctatcgaccg agattgccga aaaagggtta ttacaccatt
     3301 gccgagcgcg tgcccccacc tgcagacaac cccgtcaaag gtgatgaccg tttgcaatac
     3361 gccgaggccg agttcaatcc gcagtggtcc aatttgggcg atgtggaagt gaatattttc
     3421 caccagtggt cgatgtcccg catgcggatc gccaccattg atgcgagcaa gaagcttgtg
     3481 gtgttcagac gcggaacggg tttcgattcc cattgggccc attttcttaa gggaaaccgt
     3541 tattacatcg aaaacgtggc agatgccctc acggaaccgg gggagtttta tctggatcaa
     3601 aagaaaggcg aattgatcta tattccccgg ccaggcgaag acccgcaaag ggccgatgtc
     3661 atcgctccgc gtttggagaa cctcgtcatc tttgcctgtg acggcgccaa caaacgatgg
     3721 gtggagcatc tccagttcca tggactcatc tttgcccatt ccaactgggt gtgtcctgaa
     3781 aacggacagt tctatcccca ggcggaagtt gggctagatg gcacaatctc ggcaatcggc
     3841 gcacgcaacg tcgtcttcga tgcctgcgcg gtgatccaca cgggcactta cgccatggca
     3901 tttggtgagg gttgccgcaa ttgccaggtt gagaactgcg aactggtgga cctgggtggt
     3961 ggcggcgtta agattggcca tgcgggctcc gccaactggc agggggccag ccaaaggccg
     4021 cagggcgagg aggcccttgt ttcgcacatc actgtaaaga attgcaccat tgcccacggt
     4081 ggtcggatcc atcctgcggc ggtgggcgtg tggatcggcc attcagccta caacacggtg
     4141 gaacacaacg acatcttcga cttctactac accggcgtct cggtggggtg gacctgggga
     4201 tatgccgaca gtccggccca tcataacgcc atcaattaca accacatcta caacatcggg
     4261 cagtatgtgc tctccgatat gggtggcgtt tatacccttg gtgttt
//
LOCUS       DAOPTR010000203         4302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16500_length_4302_cov_3.815635, whole genome shotgun sequence.
ACCESSION   DAOPTR010000203 DAOPTR010000000
VERSION     DAOPTR010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16500_length_4302_cov_3.815635"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4302)
                     /locus_tag="PKI05_05210"
     CDS             complement(<1..>4302)
                     /locus_tag="PKI05_05210"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HOA51633.1"
                     /translation="ADADQLAFVAMEMAVDQPGSARLIWASGKVNGQQSTGFTLRAGE
                     RFAVYNLPVAGTSNWKTPVIYLALELTSNHRPVRLRLKWLKLSEEPGGPPDLELERFF
                     VKDALPRVDKVCNVVAHVTNRGGEPVRDVRAELILPDGAEFVDSASPLRHLAELEFGE
                     TEALVWKIKNRRVGPVSLRLRIVSPELEEYECQEEFLPDLRLPRQDYVPEPKPVRGPF
                     EVGVYYFPGWGRPSSWQPIEHFPERRPVLGFYREGLPEIADWHIKWAVEHGITFFCYD
                     WYWRQGERRLEHALHDGFFQARYRHLMKFCLLWANHFGPGEHSAEDNERVCKYWIDNY
                     FRRPEYFKVEGRPLLVIFSVHSMQRDLGIEGTRKAIELWHRMTEEAGVGKILVAGCGT
                     PANLKQMKEMGFDAVTGYNWPSCGIQDRNWVPFSEVAANYDALWWRPLAEAGAMPVIT
                     PVSAGWDSRPWHGDRALVLTDCTPQAFQQHLEDAKRFIEETGQPRVLLIEAWNEFGEG
                     SYCEPHKQYGFGHLEAIRKVFAPDSQPPLHYGPEDVGRPLLEFRDLEKPVSRTQWNFD
                     TPGQDEGWKPLMGLTDVTVANGVLAARTISGDPAFHCSTKLRAKDFAGIEITIAMRSS
                     KPTDIVQLFWATLSNTYREEASVRTTVVCDGKMRTYRLDLAGHPLWRGVITGFRLDVG
                     SQPDAEIKIDSIRLIRSASEAFENLRRAIDDLIATFGASYPEGQEFRRRLDELEQLHR
                     KGQPVEKALSELQRKALLANPLLDFDRLLVLKRKRGQLGLPTNHQCNSCLDQTGYDNE
                     LAIFSWRDGTLKTLYRPPQGFYVGEVDLHWDADRLLFTMPTGQTWQIHEMRLDGTGLR
                     RVSREEPGVDNFDACYLPDGGIAYASTAAFTAVPCWHGRERACAIFRMEADGSGVRQL
                     CFDQDLDLHPAVLPNGQIIFSRWDYTGIMHIYLRPLMVMNPDGTQQRAIYGSNSYYPN
                     ALYFPRAVPGQASKLVAILSGYHGVNRAGDLVLLDYGRGWHEAKGIVHRFMHGREPIE
                     PIIRDDLVGPNWPKFLHPYPLSENYVLVSAQLHQKGGWGIYLVDRFDNMIPILVDEQY
                     DFFEPIPIRKQPRPPVIPPRVDLSDPTAVVFLQDVYRGPGLKGVPRGTIKNLRIVAYH
                     WGFPGMAGPDKVGRAGPWDVMRILGTVPVEEDGSALFRIPANTPIAIQALDENGAAVQ
                     IMRSWYTAMPGEQTSCIGCHEPPTEAPQQLTRLVGMREPVDIKPWYGPPRGFDFEREI
                     QPVLDKYCVGCHDGQGNDGGRLDLRALRFHPDYQGLPLSQLGASRLWPEVAALLPSCE
                     PPHRLLGNKRYPYTPAYEALIPFVRRVNIEDDVYLLNPCEYSADTSELIQMLKKGHYG
                     VELDPEAWDRLQTWIDLNGPCHGRWGVVCPVPQEANKKRLAILKQSGGPDIDPEEL"
BASE COUNT          990 a         1278 c         1136 g          898 t
ORIGIN      
        1 caactcttcg ggatcgatgt cgggaccacc actctgtttc aggatggcca accttttttt
       61 gttggcctcc tgcggtacag gacagaccac gccccagcga ccatgacagg gaccatttag
      121 atcaatccag gtttgcagac ggtcccaagc ttcggggtcg agttcgacac cgtaatgccc
      181 ttttttcagc atctgaatta gttcactcgt atcggcacta tattcgcaag gattcaagag
      241 gtaaacatcg tcctcaatat tgacacgacg cacaaaggga ataagggcct catacgccgg
      301 agtgtaggga taccgtttgt tacccaggag gcgatgtggt ggctcacacg aaggcaaaag
      361 tgctgccacc tccggccaaa gacggctggc ccccagttgg gagagcggca acccttgata
      421 gtcggggtga aaccgcaagg cccgaagatc aagcctcccg ccatcattac cctggccgtc
      481 gtgacagccc acgcaatatt tgtccaaaac cggttggatc tctcgttcga aatcaaatcc
      541 tcgcggcggc ccataccaag gcttgatgtc caccggttcc cgcattccga ctagtcgggt
      601 caattgctgg ggggcctccg tcggtggctc atgacatccg atacaggacg tttgttcgcc
      661 cggcatcgcc gtgtaccaac ttcgcatgat ctgcaccgcc gccccatttt catcgagggc
      721 ttggatggcg atgggagtat tggcagggat acgaaacagc gccgagccgt cctcctcgac
      781 agggaccgtc cccaagattc gcatcacatc ccacggaccg gctcgcccta ccttgtccgg
      841 gccagccatc ccgggaaatc cccaatgata ggcgacaatc cgcaggttct tgatcgtccc
      901 ccgagggact cccttcaaac ccgggccacg atacacatcc tggagaaaga ccaccgccgt
      961 gggatcagac aaatcgaccc ggggtggaat cacagggggg cgtggctgct tgcgaatggg
     1021 gatcggctcg aagaaatcgt attgctcatc gacaaggatc gggatcatgt tatcgaagcg
     1081 atcgaccagg taaattcccc agccaccttt ctggtgtaat tgggcagaaa ccaggacgta
     1141 gttttcgctc aagggatatg ggtggaggaa ttttggccag ttcggcccaa cgaggtcatc
     1201 gcggatgatc ggctcgatcg gttctcgccc atgcataaac cgatggacaa tcccctttgc
     1261 ctcgtgccaa ccacggccgt aatcaagcag aacaaggtcc ccggctcgat tgaccccatg
     1321 ataacctgac aaaatggcga cgagttttga ggcttggccc ggcaccgccc tggggaaata
     1381 aagggcattg ggataatagg agttcgatcc gtaaatcgcc cgttgctgtg taccgtcggg
     1441 gttcatcacc attaatgggc gaaggtaaat atgcatgatg ccagtgtagt cccagcgact
     1501 gaaaatgatt tggccatttg gcaggacggc cgggtgcaaa tcgaggtcct gatcgaaaca
     1561 aagttgccga acccctgaac cgtcggcctc catccgaaaa attgcacaag ctcgctctcg
     1621 cccatgccaa cagggaaccg ccgtaaaagc ggcggtcgag gcgtacgcaa ttccgccgtc
     1681 cggcaggtaa caggcatcga agttatcgac cccaggttct tctcgggaga ccctccgcag
     1741 acctgtccca tcgagacgca tttcgtgaat ctgccaggtt tgaccagtgg gcattgtaaa
     1801 gagcaggcgg tcagcgtccc aatgaaggtc aacctcaccg acgtaaaaac cctggggagg
     1861 ccggtaaagc gtcttgagag taccatccct ccatgaaaaa attgcaagct cgttatcgta
     1921 acctgtctgg tccaggcaag aattgcactg atgattggtg ggcaatccga gctgtccccg
     1981 cttgcgtttc aacaccaaga ggcggtcaaa atctaacaag ggattggcca aaagggcctt
     2041 tctttgtaac tcgctcaacg ccttttcgac cggctgtcct tttctgtgca attgttccaa
     2101 ttcatccaaa cggcggcgaa actcttggcc ctcaggatag gacgcgccga acgtggcgat
     2161 cagatcatcg atcgcgcggc ggagattctc aaaggcctcg gacgcgctgc ggatcaggcg
     2221 gatcgaatca atcttgatct ctgcatcggg ttgggaaccg acatcgagcc gaaaaccagt
     2281 gataacgcct cgccacagag gatggccggc caggtccagt cgataggtcc gcatcttacc
     2341 gtcgcagacg acggtggttc tcacactggc ctcttcccga taggtattgc tcaacgttgc
     2401 ccaaaagagt tgcacgatat cggtcggctt cgatgaccgc atggcgatcg taatttcgat
     2461 accggcgaag tccttggccc tgagttttgt cgagcagtga aacgccgggt cgccgctgat
     2521 tgtccgggcg gctagaacac cgttcgccac cgtcacatcc gttagcccca ttagaggttt
     2581 ccatccttca tcttgacccg gtgtgtcaaa gttccattgg gtccgcgaaa ccggcttttc
     2641 cagatcacgg aattccaaca gcggtcggcc aacatcttct ggaccataat gaaggggggg
     2701 ttgactatcc ggggcgaaga ccttgcggat tgcctctaga tgaccgaaac cgtactgctt
     2761 gtgcggttca cagtaggaac cctcgccaaa ctcattccag gcctcgatga gaagaacacg
     2821 aggctgaccc gtctcttcga tgaatcgctt ggcatcctcc agatgctgct gaaatgcttg
     2881 cggagtacag tcagtcaaga ccagagcccg atcgccatgc cagggacgag aatcccagcc
     2941 cgcgctgacg ggggtaatca cgggcatggc tccggcctcc gccagcgggc gccaccacag
     3001 tgcgtcgtag ttggcagcca cttcgctaaa agggacccaa tttcggtcct gaataccaca
     3061 actgggccag ttgtagcccg tcaccgcatc gaagcccatc tctttcatct gcttaaggtt
     3121 ggccggggtc ccacaaccgg ccaccagaat cttgcccacc ccggcttcct ctgtcatgcg
     3181 gtgccataac tcgatggctt tccgcgtgcc ttcgatcccc aagtcccgct gcatcgagtg
     3241 aacggagaag atcaccaaca acggccgacc ttcaactttg aaatactctg gccgacggaa
     3301 gtagttgtcg atccaatact tacagacgcg ctcattatcc tcggcactgt gttcaccagg
     3361 cccaaagtga ttcgcccaaa ggaggcagaa cttcatcagg tggcggtatc gtgcctggaa
     3421 gaaaccatca tggagggcat gctctaatcg acgctctccc tgcctccagt accagtcgta
     3481 gcagaaaaat gtaatcccat gctccacggc ccacttgatg tgccaatcgg caatttccgg
     3541 caggccttcc cgatagaagc ccagcacggg acgccgttca ggaaagtgct cgatcggttg
     3601 ccacgagctt ggccgacccc accctgggaa atagtacacg cccacctcaa atggcccacg
     3661 tacgggcttg ggctccggca cgtaatcctg tcgcggaagt cttaagtccg gcaggaactc
     3721 ttcctggcac tcatattctt cgagttctgg cgaaacgata cgtaaccgca gagaaacggg
     3781 gccaactcga cgatttttga ttttccaaac gagggcttct gtctctccga attccaactc
     3841 cgccaaatgt cttagcggag acgccgaatc aacaaactcc gctccatcgg gtaggataag
     3901 ctcggcgcga acatctcgaa ctggttctcc cccgcgattt gtgacatgag ccacaacatt
     3961 gcagaccttg tccaccctgg gaagggcgtc cttcacaaag aaacgctcca actcaaggtc
     4021 cggcggccca cctggctctt cactgagttt caaccatttg aggcgcaatc ttaccggacg
     4081 atgattgctg gtcagctcca aagccaagta gatcaccggg gttttccagt tcgacgttcc
     4141 tgcgaccggg agattgtaca cggcgaaccg ttccccggcc cgtaaagtaa aaccagtgga
     4201 ctgctgccca ttgacctttc cagaagccca aatcaggcgg gcacttcccg gctggtccac
     4261 ggccatttcc atggcgacaa aagccagttg atcggcatcc gc
//
LOCUS       DAOPTR010000204         4302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16502_length_4302_cov_3.270308, whole genome shotgun sequence.
ACCESSION   DAOPTR010000204 DAOPTR010000000
VERSION     DAOPTR010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16502_length_4302_cov_3.270308"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..1859
                     /locus_tag="PKI05_05215"
     CDS             261..1859
                     /locus_tag="PKI05_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOA51634.1"
                     /translation="MALYEADHLFDELELRSMEDEEELEFQGRGLLEMAESYADDELA
                     EDEEAISEEAYQAAANLEESEEDTILTADEEESWSDDPVRMYLTQMGEIPLLSRQEEI
                     ALAKKIEITRAKFRRKLMECDYVLQQAVRTLARVDEGKLPFDRTVQVSVTAHLEKEQI
                     LGRLPHNLRTLQAILKRNRQDYLTALSKSKPMEERRAAWRRLGRSRRRAGRLAEELGL
                     RTQRLEPMIKALEEFSRRVDELKARIQEHANAGRPLEERKPWIKEYRDILRLTQETPT
                     SLRNRVNYVKKIYAEYQEAKRALSEGNLRLVVSIAKKYRNRGLSFLDLIQEGNAGLMR
                     AVDKFEYRRGFKFCTYATWWIRQAITRAVADQSRTIRIPVHMAETMTKVRNASRKLYQ
                     EKGHEPTLEEMAAAADAKTEETRRVLTMNRYPISLDRPVGNSEDSHFGDLLPDTSAES
                     PLDGATHDMLREKINKVLRTLSYREREIIKLRYGLGDGYCYTLEEVGHIFKVTRERIR
                     QIEAKAVRKLQQPSRSQELVGFLD"
     gene            2369..3049
                     /locus_tag="PKI05_05220"
     CDS             2369..3049
                     /locus_tag="PKI05_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51635.1"
                     /translation="MVKHSWLLFLWPGMAALWVRPTLTAWLGSAAAAVVFHLALAATI
                     LWPEWLSPQERMLVWIALGVAWLSGVAWSYQFLRREEARQHRRPAEDRFPAATEAYLR
                     GNLAAAESLVREILRECPRDVEAALLLATIWRRREQFDAAEKLLRNLARLDLAIPWLW
                     DIERELLRIQKSREEKADEPSPQPEEEQTPVEEPVAGEPPPSSQKTVPLPERVPAGRT
                     AQPATKAA"
     gene            3843..>4302
                     /locus_tag="PKI05_05225"
     CDS             3843..>4302
                     /locus_tag="PKI05_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOA51636.1"
                     /translation="MQLANQEAQRFNHEYVGTEHILLGLIKEGSGVAANVLRNLDVDL
                     KKIRLEVEKLVQTGPDMVTMGRLPLTPRARKVIEYATEEARNLNHNYIGTEHLLLGLL
                     REQEGVAAQVLMNLGLKLEEVREEVLNLLGHGSEAAETPDRNAGLGQDEGT"
BASE COUNT          961 a         1143 c         1341 g          857 t
ORIGIN      
        1 tctccgtcct gcgttgacga tgatggtgca ttggcggagt tgccgtctga aggtggtggg
       61 gaaggtttga gtagctaata ctctgttggg tggtgtcctg aacgaaaacg cacgatcctt
      121 ttcgtaactg tgtggcgcca ttgggtggaa aaacgacccc cctttggtcg tccggggcgg
      181 ccccagtcgc tcaaccggtc gtagtggtga tgggagtaaa cctaactgtt tcgggcgtgt
      241 cggtgatttt ctagaggagt atggccttgt acgaagccga tcatctgttt gacgaactcg
      301 aattgcgatc gatggaggac gaggaggaac tggagttcca gggaaggggc ttgctggaga
      361 tggccgaatc ctacgccgat gatgaattgg ccgaggacga ggaggccatc agcgaagagg
      421 cgtatcaagc cgccgccaat ctggaggagt ctgaggaaga tacgatcctc acggccgacg
      481 aggaggagtc ctggtccgat gatcccgtcc gcatgtacct gacgcaaatg ggcgaaatcc
      541 ccttgctctc gcgtcaggag gagatcgcac tggcgaagaa gatcgaaatc acgcgagcca
      601 agtttcgtcg caagctgatg gaatgcgact acgtccttca gcaggccgtg cggactctgg
      661 ctcgggtgga tgaagggaaa ttgcccttcg atcggaccgt tcaggtgtcg gtcactgctc
      721 atttggaaaa ggagcaaatc ctcggccgtt tgccgcacaa cctgaggacg cttcaagcca
      781 ttcttaagcg aaaccggcag gactatctca cggccttgag caagtcaaag ccgatggaag
      841 aacgccgggc ggcgtggcgg cgtctgggac gttcccgacg tcgggccggg cgtttggcag
      901 aagagttggg cctccgcacc cagcgccttg agccgatgat caaggccttg gaggagttca
      961 gccgtcgggt cgatgagctg aaggcccgta tccaagagca cgccaacgct ggacgaccac
     1021 tcgaagaacg caaaccgtgg atcaaggagt atcgtgacat cctccggctg acgcaggaga
     1081 ccccgacgag tctgcggaat cgcgtcaact acgtcaagaa aatctacgca gagtaccagg
     1141 aagcgaagcg ggccttgtcc gagggaaacc tccgcctggt ggtttccatc gccaagaagt
     1201 accgcaatcg cggcctgagc ttcctcgatt tgattcagga aggcaacgct ggtctcatgc
     1261 gggcggtgga taagttcgaa tatcgccgcg ggttcaagtt ctgcacctat gccacgtggt
     1321 ggattcgtca ggcgatcacc cgcgcggtgg ccgaccaaag ccggaccatc cgtatccccg
     1381 tccacatggc cgagaccatg accaaggtcc gcaacgcctc gcgaaagctc tatcaagaga
     1441 agggccatga accgaccttg gaagagatgg ctgccgccgc cgacgccaag accgaggaaa
     1501 cccggcgtgt cctcaccatg aaccgctatc cgatcagtct cgatcggccg gtgggaaata
     1561 gtgaggatag ccatttcggg gaccttctgc cggacacctc tgcggaaagc ccgctggatg
     1621 gtgccaccca cgatatgctc cgggaaaaga tcaataaggt gctgcggacc ctcagctacc
     1681 gtgagcggga gatcatcaag ttgcgctatg ggctgggcga tggctactgc tacaccttgg
     1741 aagaagtggg ccatatcttc aaggtgaccc gtgagcgtat ccgccaaatt gaggccaagg
     1801 ctgtgcgaaa gctccagcaa ccaagccgca gccaggaatt ggtcggattc cttgattgaa
     1861 aggcttaggg cgttttcatt tcgccctgct gcccacaaaa cgtttccgta ttggttttga
     1921 gcgaccctcc acatcgtagg ggcgggcctc tgtgcccgcc ccttttgtta tgtggacggt
     1981 cacgcgccgt cgcggccggg aaataggaaa catgagcggg ccatcaacca gcaccttggc
     2041 cgatgcaatg gcgtgtcact ctcgatcttt tgcgccgatc ctccccagcg ggtccgtttt
     2101 cgttttgcca ttcgcgccgg ttgactttgt attaaaaacg gcaatcagac ggcagccgtc
     2161 cgatcaaaat tgtcggttat ggataagcaa ggtgagcgcg tggctacggt gaggatgagt
     2221 gacgaaaaaa gcgcttgcgg gcgagtttgc gattgaagca gtctggctga cgggctagaa
     2281 ttggcttcag aattggaatg gtgcggcgaa caaagtcccc gcggccgcgg ttgacgtgcg
     2341 gccgtttgtt gaagaattaa ggtcacggat ggtcaaacac tcctggcttc tttttctttg
     2401 gcccgggatg gcggccctgt gggttcgtcc cacgcttacg gcctggctgg gaagcgcggc
     2461 ggctgccgtg gtcttccacc tggccctggc ggcaaccatc ctttggccgg agtggctttc
     2521 gccgcaagag cgaatgcttg tgtggatcgc cctaggggtg gcctggctca gcggggtggc
     2581 ctggtcgtat cagttcctcc gccgagaaga ggcacgacag caccgtcggc ctgccgagga
     2641 ccgtttcccg gcagctacgg aagcctatct ccggggaaat ctcgcggcgg ccgagtccct
     2701 ggtgcgggaa attctgcggg aatgtcctcg tgatgtcgag gcagctttac ttctggccac
     2761 aatatggcga cgtcgggaac aattcgatgc cgccgagaaa ctgctgcgaa acctcgcccg
     2821 gctggacttg gccatcccct ggctttggga catcgaacga gaacttcttc gaatccaaaa
     2881 atctcgtgaa gaaaaggccg atgagccctc gccacaaccc gaggaggagc aaactcctgt
     2941 agaagaaccg gtggccgggg aaccgcctcc ttcttctcaa aagaccgtcc ccttgcccga
     3001 acgagtgccg gcagggagga cggcccagcc ggccaccaag gccgcctgag gtgcgtcgct
     3061 tttagagcca atcccaaaaa agtcgccctc tccctccggg agagggttgg ggtgagggca
     3121 agcgaacgtc agaattgtat tgagattgag aaaattggca gcgattcgag ccactctcaa
     3181 cccgactcct ctcccgctga acgggagagg ggagattttg agacacgctt ttagccaaac
     3241 cagtctggtc ggtgcctaac gtgatcgggg gttttggggg catatgagaa atctctgtgc
     3301 gaatagcaca acagatgcga gaaatcagat ataattcggt ggagccccga agacttggcc
     3361 gcggggcgaa cccggtgtgc caagcttcgg aggatgggaa ctaagcccaa caagccctgc
     3421 gctgtcatca tgactacgca gaactgccac aatgacagtt tgccaaaatg gagggatgga
     3481 gtaaactttg gcaggcaagc aggcatcggc ctgcacataa tcagcccagt gtgcgtagaa
     3541 atttacgaag gttttggttg tagaaaatca cgtagtgcgg ttttgggcgg gttatcccgg
     3601 gccaaaacaa gttttgatgg tacgggagtt gcaacaatgt tctgggtggg tcagcccacc
     3661 tgacaagttg acagcaactt ggtgcagggg tgtcctgccc aagtgcggga aggggattta
     3721 aaagaaacac aagcgaggtt gtcgtcccag tggtaagttg gcgaagcctt cgcatcattt
     3781 gagttaagga gttttgagag attatggcgt tcgaaagatt caccgatcgt gcccgaaagg
     3841 tcatgcagtt ggccaaccag gaggcgcagc gcttcaacca cgaatacgtg gggacggagc
     3901 acatcttgct cggtctcatc aaggaaggca gtggtgtcgc tgcgaacgtc ctgcgcaatc
     3961 tcgacgtgga tctgaaaaag atccgccttg aggtggaaaa acttgtgcaa acaggaccgg
     4021 atatggtcac catgggccgt ctgccgctga cgccgcgggc ccgcaaggtg atcgagtacg
     4081 ccacggaaga ggcgcgaaac ctcaatcaca actacatcgg cacggaacac ctcctcttag
     4141 ggttgctccg cgagcaagaa ggtgtcgccg cccaggtcct catgaatctc gggttgaagt
     4201 tggaagaggt ccgggaagag gtcctcaacc tcctcggtca cggcagtgag gccgccgaga
     4261 ctcccgatcg caacgcaggg ttgggtcagg atgaaggaac gg
//
LOCUS       DAOPTR010000205         4297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16529_length_4297_cov_3.925507, whole genome shotgun sequence.
ACCESSION   DAOPTR010000205 DAOPTR010000000
VERSION     DAOPTR010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16529_length_4297_cov_3.925507"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            692..1489
                     /locus_tag="PKI05_05230"
     CDS             692..1489
                     /locus_tag="PKI05_05230"
                     /inference="COORDINATES: protein motif:HMM:NF014638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HOA51637.1"
                     /translation="MLVLTRRENERIVLPTLKTFIQVVRIAGSTVRLGIEAPANVPVL
                     REELLKENKAPEDAARKPQVPCLDSFPETLEHSHDAASAPATRLSQQEKLLDCLALAC
                     DLADTHLANGKVAEARETIQKALELLRRLGARSVVAASSPVRVSGQRVALVIEPTKSE
                     GSSMSQVLRKAGYSVISFASGCRAIDYLASHNRPDLIVINLQENRPCAADTIRMIRQN
                     PALRGVKVVALSDSRPAEAEISIGPQGVDYWFATQTCSERILRQIEA"
     gene            1580..2425
                     /locus_tag="PKI05_05235"
     CDS             1580..2425
                     /locus_tag="PKI05_05235"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HOA51638.1"
                     /translation="MIERDTLFRLSDGSSLRGRSWEPEGNPRARIFGLHGVLEHSGRY
                     GRLATDLTQAGYALEMVDLRGHGRSDGPRAFIPSWEQLLVDLDEVHAQLRAEVRVPDF
                     LFGHSMGAALTILWVTSRRPNIKGMILSAPPVMTPVRVPMLVRIVGRWLVHRWPELRI
                     VRLKTAERYGRHAVSRDPQVLEDLKNDPLVCRGPFPLKTGLLLLELPQRLQEAARACD
                     APFLLLQGTGDKLSSYRGAELFFQLAPARDKVLRLYEGLYHEVLSEPERAQVLADVLS
                     WLSAH"
     gene            2769..3197
                     /locus_tag="PKI05_05240"
     CDS             2769..3197
                     /locus_tag="PKI05_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51639.1"
                     /translation="MKRLLALLLGIGLLVYVTGCPKPATKPEGGTGPAPSTTAPAGPA
                     EEQPAEQPAEQPAEQPAEQPAEQPAAGPELPAPAAPAEVTMPAPPAEQPAQQPAEEPA
                     AQPAQPAEQPAPAAEQPAPQPEQKPEEKPAEQPAEQPAQQ"
     gene            3514..>4297
                     /locus_tag="PKI05_05245"
     CDS             3514..>4297
                     /locus_tag="PKI05_05245"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOA51640.1"
                     /translation="MPTLRTVTLGCKVNQYETEYLRQGFLRLGYQDAENGEPADLCIF
                     NTCTVTAQADSECRKILRRLARENPHAEIIVMGCFATKAAEEIRSLPGVSEVLTDKRQ
                     MGDLLARRGLVDIPTGISGFAKRHRAYVKIQDGCVFPCSYCIIPRVRGRLWSRPLREI
                     LNEVAQLVDNGYREIVLTGIHLGLYGHGPEDTDLPHLAGLVEAIIDSRSGFRVRLSSL
                     EVNEVTDSLLDLMAASPERICPHLHIPLQSGSNRVLKRMRRRA"
BASE COUNT          906 a         1236 c         1250 g          905 t
ORIGIN      
        1 ttcggcggga gaggggtcgg gggtgagggt ggctcgaatc gctgccggtt ttctcgatcg
       61 actccgacgg tcccttgccc tcaccccaac cctctcccgg agggagaggt ggctctttgg
      121 gatggactct tacttcctag gctaatgggc aaaatcgttg cggcagtagc ccccaggctg
      181 ccatggaatc cactccaaaa aattgatctt ggccggtttt gctggatttg gtaaaacatt
      241 ccacgatcgc cagattatgc gttcttctct agacaaggat gtcttgcgtg cgggcgaacc
      301 aaggacggga acgcccggcg gtccggccag atgattcgac ggcaacggct gaatccaaag
      361 cgttcgccat tcgcgaggat caactgagga gtctcgcagt aagcctgaac cgtgccgttc
      421 tgcgggggat atgtgcatct gcaagatcgt gcctcgaacg tctgtgaagg atttggcaag
      481 cgggatccaa gtgtagaacc cggcccacac aggccacgtg gacaagtagc aagggaaaaa
      541 cggaccgtca attaaggctt gcgcatagag gatgagaacc ggagccgcgc agccgctcgc
      601 actgcctgcc gcatttgtga gcgcgatccg ggttgcggaa ggtggagact ggattcgtgg
      661 tgcaacccca agatgcgtag agggaacgac catgttggtg ctaactcgcc gagaaaacga
      721 acggattgtc ctgcctactc tcaaaacttt tatccaggtg gtgcggattg ccggctcaac
      781 agtccggtta gggatcgagg cgccggccaa cgtacctgtt cttcgggagg aacttctcaa
      841 agaaaacaaa gcacccgaag atgctgccag gaaaccccag gtgccgtgtt tggattcctt
      901 ccctgagacg ctcgagcatt cgcatgacgc cgccagtgcc ccagcgaccc gtctgtcgca
      961 gcaggaaaaa ctcttggatt gccttgcctt ggcctgcgat cttgcggaca cccatttggc
     1021 aaatggcaag gtggctgagg ctcgcgaaac aatccaaaag gccctggagc ttcttcgtcg
     1081 cctgggggcg agatcggtgg tggctgcttc ctccccggtt cgggtctccg gtcagcgcgt
     1141 tgcccttgtc attgagccga cgaaaagcga gggctcttcc atgtcgcaag tgttgcgaaa
     1201 ggcgggttat tcggtaattt ccttcgccag tggctgccgt gcgattgatt atcttgcctc
     1261 gcataaccgg cccgatctaa ttgtgatcaa cctacaggaa aaccgacctt gcgcggccga
     1321 taccatccgc atgattcgcc aaaacccggc gctccgcggc gtcaaggtcg tggccctgag
     1381 cgattctcgt ccggccgagg ccgaaataag catcggtccc caaggcgtcg attactggtt
     1441 cgccacccaa acctgctccg aacgaatcct ccgacaaatt gaggcataag cgatttttca
     1501 actgttttcg tcgtgtctcc gcgcggcgtc agaccgctta acgagtcacc aacgaattgg
     1561 caacgaatgg acgtgcaacg tgattgagcg ggatacactg tttcggctat ctgacggttc
     1621 ttcgttgcgt gggcgatctt gggaaccgga gggaaaccct cgtgcccgaa tcttcggcct
     1681 ccacggtgtc ctggagcata gtggccgata cgggcgattg gccacggatt tgacccaagc
     1741 cggttacgca ttggagatgg tggaccttcg cggccacggt cggtccgatg gcccacgtgc
     1801 cttcattcca agctgggagc aattactggt tgatctagac gaagtccatg cccaattgcg
     1861 ggcggaagtg cgagtgccgg actttctttt tggacacagt atgggggcgg ccctcacaat
     1921 cttgtgggtg acgtctcgcc gaccgaacat aaaagggatg attttgagtg ccccaccggt
     1981 catgacccca gttcgggtgc ccatgctcgt gcgtatcgtt ggccgttggc tggtccatcg
     2041 ctggccagag ttacgcatcg tccggctgaa aacggcggag cgatatgggc gacacgccgt
     2101 gtctcgcgat ccccaagtcc tggaagattt aaagaacgac cccctggtat gtcgtggccc
     2161 tttcccgtta aagactggtc tactacttct ggagttgccc caacggttgc aggaagccgc
     2221 ccgggcgtgc gacgccccgt ttcttcttct ccagggtacg ggggataagt tgtcgtccta
     2281 tcgaggggca gaattattct ttcaacttgc tccggcaagg gataaagtcc tcaggttata
     2341 cgagggcctg tatcacgaag tcctcagcga accggaacgt gcccaggtgt tggcggacgt
     2401 cttgtcttgg ctttccgcgc attagtcgcc cgaaaaagat cttcctgcgg aaaataggta
     2461 gccaacgcgc gggcggatat ctgaatcatt ttgatatcac ccacaatggg cgggggaata
     2521 attggctttg aagcgtgatc tatattggtg tagggtccag gttcccactt tgcaggcgca
     2581 gaacagccat tgatggcgat ctcctaatgg acacggcctc atggaatggg taccttaaaa
     2641 agggcgagtc ggacaaaccc ccgctcatag aaatacacga accggcggga ttgttttcaa
     2701 ctgaccgagt tggtcggtgg ccgccggttt gaggagggtt aactcaatct gttgtggagg
     2761 tctaactcat gaagcggtta cttgcattac tgttagggat cggcttgttg gtttacgtga
     2821 caggctgtcc gaaaccggca acgaagccag aaggcggcac gggccccgcg ccctcgacca
     2881 ctgccccggc aggccccgcg gaagagcaac cggctgaaca gccggctgaa caacccgcag
     2941 agcaaccggc cgagcagcca gcggaacaac cggctgccgg tcctgagttg ccggctcccg
     3001 cagcgccagc cgaggtgacc atgccagctc ccccggcaga acaaccagcc caacaaccgg
     3061 cagaggaacc agccgctcaa ccggctcagc ccgctgaaca gccggcccct gctgccgagc
     3121 aaccggcacc tcagcctgag caaaagccgg aagagaagcc tgccgagcaa cctgctgagc
     3181 aacccgccca gcagtgatcg caaatcggtc agactagtga tctgactcta ccgactgagg
     3241 tctgccctcg ttcgcaggcc gggtaaatgt tgcaaattta ccgggagtat tggtttagcg
     3301 agcctctcac taaggctcgc ttttttgttg agaaccgcca accgggaata aagcccctga
     3361 tcggaggcca ctccaatatc ctgtccgcgg ccggagaaca tccgccaacc ccaccccctg
     3421 atgaccgggc acggccacag ctcttctcct gtttgataca ataaactttg cgattgaaac
     3481 ccctcccttt tgagactgaa ttcttccacg cttatgccga cgctccggac ggtcacgctg
     3541 ggttgcaagg tcaatcagta cgagaccgaa taccttcgcc aaggcttttt gcgactcggc
     3601 tatcaggatg ctgagaacgg ggagcctgcg gacctctgta ttttcaacac ctgtaccgtc
     3661 acggcccagg cagacagcga atgtcggaaa atcctccgcc gccttgcccg cgagaatccc
     3721 cacgccgaga tcatcgtgat gggctgcttt gccacgaagg cggccgagga gatccggtcc
     3781 ctgcccggtg tgtcggaggt ccttactgat aaacgccaga tgggcgatct cctggcccgc
     3841 cggggccttg tcgatattcc cacgggaatc tcgggtttcg ccaagcggca tcgggcatac
     3901 gtcaagattc aggacggttg tgtctttccc tgcagttact gcattattcc acgggtacgg
     3961 ggacggctgt ggagccgccc gctgcgggag atcctcaacg aagttgccca attggtggat
     4021 aacggctatc gggaaatcgt cctcacggga atccatttgg gactgtacgg ccacggtccg
     4081 gaagataccg acttaccgca tttggcaggg cttgtggaag ccattatcga cagccgcagc
     4141 gggttccgcg tccggctttc cagtctggag gttaacgaag tcacggatag tcttttggac
     4201 ctcatggcgg cgtcgcccga acggatttgc ccccatttgc acattccctt acaaagtggt
     4261 tccaatcgcg tccttaaaag gatgcgacgg cgtgcca
//
LOCUS       DAOPTR010000206         4285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16581_length_4285_cov_3.925296, whole genome shotgun sequence.
ACCESSION   DAOPTR010000206 DAOPTR010000000
VERSION     DAOPTR010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16581_length_4285_cov_3.925296"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PKI05_05250"
     CDS             complement(<1..188)
                     /locus_tag="PKI05_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51641.1"
                     /translation="MCGRFTLRAAPQEVAKHFSLFDVPTFSPRYNIGPGQVVGAIRAA
                     ALNAPQAVAISDDMKLQP"
     gene            550..1422
                     /locus_tag="PKI05_05255"
     CDS             550..1422
                     /locus_tag="PKI05_05255"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOA51642.1"
                     /translation="MAIPGLTIIGESINDSVPSTKALFDAGDLEGIVALAKSQVEGGA
                     AYIDVNVGSRGPELMAEVVRRVQGAIDKPLSIDTPDPVLARAGLTTYSPERAGGQKPI
                     LNSITVSREEMWELYALQPFCPILLCTEILVDGRPQAARTAEQTYEAAKFLVAEARRR
                     CPGIPNSDLIIDPGIVPIASDTEGNLKRLLGAMELIHNDPDLAGVHMSVGLSNFTVML
                     PPKRKDGSPVKSALESAFLTKAIPLGLDMIIGSVKRKYELLPPDHPAMQCLDAVLQAA
                     GFEAIMAVRKFYAE"
     gene            1526..2404
                     /locus_tag="PKI05_05260"
     CDS             1526..2404
                     /locus_tag="PKI05_05260"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOA51643.1"
                     /translation="MPTSVLDELLKSAPVVLDGAWGTQLQMRGLPVGACPDEWNLSHP
                     ELVEEVARAYVAAGSQIILTNTFGSNPVTLQRHGLADQVVAINRAGVEISRRAAAGRA
                     KVFASIGPSGVMLMMGEKTPDELRAAFAVQAEALAQAGADGLVIETMSDPAEALLALE
                     AARATGLPVVVCMTFDSGKNRDRTMMGTTPEQAAEQLTAAGADVIGANCGAGPENYVE
                     ICRRLKAATDRPIWIKPNAGLPEVVNGQTVYRLSAEDFSKYVPQLVDAGASFIGGCCG
                     TTPEFIRAICRVLGRT"
     gene            2447..3679
                     /locus_tag="PKI05_05265"
     CDS             2447..3679
                     /locus_tag="PKI05_05265"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOA51644.1"
                     /translation="MNSRERVLCALNHREPDRVPIDLGGTRQSGIAATAYHRLKERLG
                     LRTPTRVYDVYQMLAEVERPILERFGADVIGLHRRCVAFGIPNQDWKPWRLFDGTPVE
                     VPGEFNPVTELDGSLVLYNKSGEPIARMPKDGFYFDRLDKYPGAAHVDPEAQEIPVIS
                     QQECDELGAQAEALFHNTDFAIIVALGPPYELFFGLGTGDFTAWMITLATEPDYVRAL
                     YERITDVWLENLRRLTAALGDHVHIIQFNDDLGTQDGPFLSPRLFKELIFPYYKRGLD
                     WIHQNTNYKVFMHNDGAIYDFIPILIEMGVDILNPVQTTAKGMDPVRLKKEFGDRLTF
                     WGAACDCQRTLPFGTPEDVAREVEANIRVLAPGGGYVFASVHNIQANCPPENIIAMFD
                     TARTVGRYPIGVPQDQNS"
     gene            3701..4219
                     /locus_tag="PKI05_05270"
     CDS             3701..4219
                     /locus_tag="PKI05_05270"
                     /inference="COORDINATES: protein motif:HMM:NF019413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HOA51645.1"
                     /translation="MRLIAITCEILYREMCLAVARSRNTVDLVFLPKGLHDVGQEKMS
                     ARLADAIAQVDTSRYEAICLGYALCNNGIVGLTAPQIPLVVPRAHDCITLFLGSHRRF
                     LEYFNANPGVYFQTPGWIERGEDLSQHGPDSIVRKLGLLQTYEEWVAKYGEENARYLQ
                     EQLGELTRNLEN"
BASE COUNT          931 a         1201 c         1231 g          922 t
ORIGIN      
        1 tcgggttgca atttcatgtc atcagaaatg gcaacggctt ggggtgcgtt cagggccgct
       61 gcgcggatgg caccgacgac ctgtcccggc ccaatgttgt agcggggtga aaaggtggga
      121 acatcgaaca acgagaaatg tttggcgacc tcttgcgggg ccgcccgaag agtgaatcgt
      181 ccgcacatag gctttcctta cggcatctgg cgatgggccc gtccgccctg tacccccatt
      241 gtatcgccca ctccgccaga cgcgacggcg cccgtccgcc aaagtttatc gtagcggctg
      301 ccctccatgc ctgcccattt cttccctggc gtcggcgggg gtgcaagaaa gcccgcgagc
      361 cacaaaggtt acccctacca gtcgatcacg atcatcctgg gagccgtgca ccgtcgtagc
      421 cggggacgac aatggacatc attggtacga cggcctatcg gcctgtggaa aggccttcca
      481 aaagatgcta aaaaaacgat ttaccttgca gttgacagcc gggtgtgacg ttaggatagg
      541 agacacgcga tggccattcc gggattaacg attattgggg aatcgatcaa cgattctgtc
      601 ccctcgacga aggccctttt cgatgcagga gacctggagg gaatcgtggc cttggccaag
      661 agccaggttg aaggcggcgc tgcctatatc gatgtcaatg tcggctcgcg cggtccggaa
      721 ttgatggcgg aggtcgtacg gcgtgtccaa ggtgccattg acaaaccgtt gtcgatcgac
      781 acgccggacc cggtccttgc tcgggcaggc ctaacgacct actcgccgga acgggctggt
      841 ggccaaaaac cgatcctcaa ttcgatcacc gtctcccgag aggaaatgtg ggaactttac
      901 gcccttcaac cgttctgtcc tattctgctt tgcacggaaa tcctagtaga tgggcgtccc
      961 caggcagccc ggacagccga gcaaacctac gaggccgcaa aatttctcgt tgctgaggcc
     1021 cgtcgtcgtt gtccgggcat ccccaacagc gacctgatca tcgaccccgg gattgtgccc
     1081 atcgcaagcg acaccgaagg aaatctcaaa cgattgttgg gcgcgatgga gttgattcac
     1141 aatgatcctg acctagcggg ggtccacatg tcggtgggtt tgagcaattt caccgtgatg
     1201 ttgccaccaa agcggaagga cggttcgccg gtgaaaagcg ccctggagtc ggccttcttg
     1261 acaaaagcca taccattggg tttggacatg atcatcggct cggtcaaacg aaaatacgaa
     1321 ttattgccgc cggatcaccc ggccatgcaa tgcttggacg ccgtcctcca ggccgcgggc
     1381 ttcgaagcga ttatggccgt gcgaaagttt tacgccgagt gacgtcgtat ccaagcgatg
     1441 tcctaccgca aggatcggca attgtggatc ttgctccaga cgacaaataa tgccatcacg
     1501 aaagctggga gaaaggacct cacctatgcc aacgtcggtg ctcgatgaac tcctgaaaag
     1561 tgccccagtg gtgctagacg gcgcttgggg gacgcagctc caaatgcggg gcctaccggt
     1621 gggcgcatgt ccggatgaat ggaatttgtc ccatcccgag ctggttgaag aagtcgcgcg
     1681 ggcctacgtg gcggccggaa gtcaaattat cctgaccaat acgtttggct ccaatcccgt
     1741 gaccctccag cgccacggcc tggccgacca ggtggtggcg attaaccggg ccggcgtgga
     1801 gatttcacgc cgtgcagccg caggaagggc aaaggtcttt gcatccatcg ggccatccgg
     1861 ggtcatgctc atgatgggcg aaaagacgcc ggacgaactg cgggcggcct ttgccgtgca
     1921 agcagaggcc ttggcccaag ctggggccga tggcctggtg atcgagacga tgtccgaccc
     1981 cgccgaggcc cttctcgcct tggaagcagc ccgggcgacc ggtttgccgg tagtcgtttg
     2041 catgaccttc gactccggaa agaaccgtga tcgtaccatg atgggcacaa caccggaaca
     2101 ggccgcggaa caattgacgg cagcgggtgc cgacgtcatc ggagccaact gcggtgccgg
     2161 tcccgaaaac tacgtggaaa tctgccgccg tttgaaggca gccaccgatc gtcccatctg
     2221 gattaagccc aacgcgggtt tgccggaggt tgtcaacggt cagacggtct atcgcctttc
     2281 tgccgaagat ttctcgaagt acgttccgca actggttgac gccggggcca gtttcattgg
     2341 tggatgttgc ggaacaacgc ccgagttcat tcgggcgatt tgccgggttc taggtcgtac
     2401 ttagggacgc ttcttttttt caccagtttt ttgcggacca ggaacgatga actctcgtga
     2461 acgagtcctc tgcgccttaa accatcggga acccgatcgc gttcccatcg acttgggtgg
     2521 aacgcgccaa tcggggattg ctgccacggc atatcatcgt ttgaaagaac gcttaggact
     2581 acgcacacca acccgcgttt atgacgtgta tcagatgctg gccgaggtcg aacgacctat
     2641 cctggaacgt tttggagccg acgtcatcgg actgcatcgc cgctgcgtgg ctttcggcat
     2701 tcccaatcag gactggaagc catggcggct tttcgacggc acaccggtag aagttcccgg
     2761 cgaattcaac cccgtcacag agctcgacgg ttctttggtg ctgtataaca agtcaggaga
     2821 gcctattgcc cgcatgccca aggacggctt ctactttgac cgtttggaca agtatcccgg
     2881 ggctgcccat gttgatccgg aagcccagga aatcccggtg atctcacaac aagaatgcga
     2941 cgagctgggc gcccaggcgg aggccttgtt ccacaatacg gatttcgcca tcatcgtcgc
     3001 tctcggaccg ccttatgagt tattctttgg ccttgggacg ggcgacttca cggcatggat
     3061 gatcaccctt gccaccgaac cggattacgt tcgcgccttg tatgagcgaa tcaccgatgt
     3121 ttggctggag aatcttcgcc gattgacggc ggccctcggc gaccatgttc acattatcca
     3181 gttcaacgac gacttaggaa cgcaggatgg ccccttcctt tcacctaggc ttttcaaaga
     3241 actgattttt ccttactaca agcgcggcct tgactggatt caccagaata cgaattataa
     3301 agtcttcatg cataatgacg gcgcgattta tgatttcatc cccatcttga tcgaaatggg
     3361 tgtcgatatc ctcaatcccg tccaaaccac ggcaaagggg atggatcctg ttcgattgaa
     3421 aaaggagttc ggcgatcgcc tcaccttttg gggtgccgcg tgtgattgtc agaggacgct
     3481 gcccttcggg acccccgagg acgttgcccg cgaggttgaa gccaacatcc gggttttggc
     3541 acccggtggc ggctatgtgt tcgcctcggt acacaacatc caggcgaact gtcccccaga
     3601 aaacatcatt gccatgtttg atacggcccg aactgtcgga cgttatccga tcggagtgcc
     3661 gcaggatcaa aacagttgaa tcttttgggc ggactaagaa gtgcgtctca ttgcgattac
     3721 atgtgaaatc ctttaccgcg agatgtgtct ggcggtggcg cggtctcgca acaccgtcga
     3781 tcttgtcttt ttgcccaagg ggttacacga cgtcggccag gagaagatgt cggctcgtct
     3841 cgccgacgcc attgcccaag tggacacgtc gcgatacgaa gcaatttgcc taggttacgc
     3901 cctttgcaat aacggcatcg tcggtttaac ggccccgcaa ataccgctag ttgttccccg
     3961 ggcacacgat tgcatcacct tgtttttggg aagccatcgg cgcttcctgg aatactttaa
     4021 cgcaaacccc ggggtgtatt ttcagacgcc gggttggata gagcggggag aagacctctc
     4081 ccaacacgga ccggactcga tcgttcgcaa gctgggactt ttgcaaacgt atgaggaatg
     4141 ggtcgccaag tacggtgaag aaaatgcacg ttatttgcag gaacaacttg gcgagttaac
     4201 gagaaacttg gaaaactgac atatatccgt acccacacgg gctgcgatga caggtttgag
     4261 gaaatcgcca gacaaaaggc gtcgc
//
LOCUS       DAOPTR010000207         4282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16595_length_4282_cov_3.738349, whole genome shotgun sequence.
ACCESSION   DAOPTR010000207 DAOPTR010000000
VERSION     DAOPTR010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16595_length_4282_cov_3.738349"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1707)
                     /locus_tag="PKI05_05275"
     CDS             complement(<1..1707)
                     /locus_tag="PKI05_05275"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOA51646.1"
                     /translation="MRCTTMLYRTAVLTLTAITACIAIPFPALAQPWNCHVIDGTISG
                     ADGVRIADANGDGLMDVVTGFEQAGQTRVYINPGPKRVHQPWPFVTVGSTPDVEDAVF
                     ADIDSDGRPDVVSCCEGRTRRVFVHWAPGPEQDYLNAKLWQTVELPLPEHDTMWMYCL
                     PLDLDGQHGLNLVLGGKQTDSGKGAALGIAVTPAEPRNVTEWRWIELTRVGWIMSIEA
                     LDIDRDGDVDLIYSDRYSVKENGGEPSGVYWLENPGSEKIGDPWKKHPVGAIGRQVMF
                     IDVGDFDSTGRLQVFAAVKPNEIHHLVAPTDPRQPWQVKEVIKVTGADFEVGTSKAVR
                     ARDLNGDGRFDLAFTCEGATGQRRGVVWLEQTEKGWQVHDLSGPVGIKFDRIEFVDLD
                     GDGDFDLLTTEESEATSTPGVAGLGVVWYENPAKGSSAAARPQPALPEGNAPQPVSFP
                     YFGHPSHAVVWRNWGLISAEDLARVLNTTPENIRNLAQAMGLPAHPALAPDFRRRGYI
                     TLVRRNWHLLPYDQLLDLLGMTAEELAYSLREDDFLFVKLGNHKPRCERVVFQAPDET
                     VAK"
     gene            2219..4072
                     /locus_tag="PKI05_05280"
     CDS             2219..4072
                     /locus_tag="PKI05_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51647.1"
                     /translation="MSLYPSAVARIVPLGRSGLTTLGILLGLLYMMMTHVAVARAENN
                     PNSRVIRHSDVVFMYDNPDLYEAYGCTVLGWAGARSPERIALAHQRGVRLFAVSVGFR
                     TEFARMIDFTPDFLQAACRNYAGDTFIVPWLWDHKHKGQPAWWFCTNHPLYRQYLMKR
                     LEETVASGADALHIDDYTGTASAVTWLDACFCSACMAGFRTYLKTKLSAERQKELGIT
                     DLDTFDYRQFLMNRGITPERYRKERPGLPLATEFLDFQVQAATDFVAEYRRKGEELKG
                     GPLPLAVNSGLTSPLSLAITPHVSYFCCEVHHAAESRKVPFHPIYVYKLADGLNRPVT
                     STASGGDWAYIMENNLPGLVRTWVALSYAFGHNLMAPHRQWCYTQKKGTHWYSGPTEE
                     YAPLYRFVREHASILDDFEAVGRIAVLYDNHSRRLGKGDIERITMSLADANWPFKVLV
                     QGNDWLPGYRVDPTTLGQFDKVIVPEDLQPLPEIQAMLDQARSQGKLVTWSAGDNLAL
                     LGKNPVQVSSPGAVFIVPRVKTTGEHPVMAIHLVNRQYDGEKDKMIPLTNLTLTIDET
                     VLPGRADGCRSAKLHSPGKEDMTVVVKPEAKHLTVTIPEIDLWTIVELEWR"
BASE COUNT          938 a         1234 c         1170 g          940 t
ORIGIN      
        1 ttttgcaacg gtctcgtcgg gagcttgaaa caccacacgc tcacaacgcg gcttgtggtt
       61 gcccaacttc acaaacagaa aatcatcctc ccgtagagaa taagccagtt cctcagcggt
      121 cattccgagg aggtcgagca attgatcgta cgggagtaaa tgccaatttc gtcgaacgag
      181 tgtaatgtaa ccacggcggc ggaaatccgg cgctagcgct gggtgggcag gcaggcccat
      241 cgcctgcgcc aagttgcgaa tgttctccgg cgtggtgttc agcacgcgag caagatcctc
      301 cgccgaaatt aaaccccaat tccgccacac cacggcatgg gaggggtggc cgaagtaagg
      361 gaaagagact ggctgagggg cgttaccctc gggcaaggcc ggttggggac gggccgctgc
      421 cgatgaacct ttcgcgggat tttcgtacca caccacgccc aaccctgcca ctccgggggt
      481 tgaggtcgct tcgctctcct cggttgtaag gaggtcaaaa tccccatcac catcgagatc
      541 gacaaactcg atgcgatcaa acttgatgcc caccgggcca ctcagatcgt gcacttgcca
      601 acctttttcg gtttgttcca gccacacgac ccctcgccgt tggccggttg ccccttcgca
      661 cgtgaacgcc agatcgaacc ggccatcacc gttcaggtcg cgcgctcgca ccgccttgct
      721 ggtgcctact tcgaaatccg cacctgtaac tttgataacc tctttcactt gccaaggttg
      781 acggggatca gtgggagcca caaggtggtg gatttcattc ggcttaaccg ccgcgaacac
      841 ctgaagtcgg ccggtgctgt caaaatctcc cacgtcgatg aacattacct ggcgaccgat
      901 cgccccaacg gggtgttttt tccaaggatc cccgatcttt tcggaacccg gattttccag
      961 ccagtacacg cccgacggtt caccgccatt ttccttgacc gaataccggt ccgaataaat
     1021 gagatcgaca tcaccatcac ggtcaatgtc gagggcttca atactcatga tccagccgac
     1081 ccgcgtcagc tcgatccaac gccactcagt cacgttgcgg ggctcagcgg gcgtcacggc
     1141 aatccccaag gccgcgccct tacctgagtc cgtttgtttt ccccccaaga cgaggttcag
     1201 gccgtgctga ccatcaaggt caagcggcag gcaatacatc cacattgtgt cgtgctccgg
     1261 caacggcaat tcgacggtct gccagagttt tgcattaaga taatcctgct ctggtcccgg
     1321 tgcccaatgg acaaagactc gccgcgtgcg accctcgcag caggagacaa cgtcaggccg
     1381 accgtcgctg tcaatgtccg caaagacggc gtcctctacg tcgggtgtgg agccaaccgt
     1441 tacgaacggc cacggttggt gcacacgctt cggcccggga ttgatgtaca cccgcgtttg
     1501 gcctgcttgc tcgaaacctg tcaccacatc cataagtcca tcgccattcg catcggcaat
     1561 gcgaaccccg tccgcacctg aaatggtgcc gtcaatgaca tggcagttcc acggctgcgc
     1621 caacgcagga aatggtatgg cgatgcatgc ggtgatggcc gtcaaggtca gaacagccgt
     1681 tcgatacaac atcgtggtgc agcgcataac agcctccttt gggtggcgtc ctataatgcg
     1741 agggaagcca tcctgcatag tcacgcccgc ttgcaggata caagcttccc cgcaggtctg
     1801 agaacgatcg tgcgatggtc gttcacagat gttctttctg tcacccacgc ggtttttcgg
     1861 tggacgtcgg cgattttggg cgccggggtc cgccttgtca agcgctcgcc ttgaagattg
     1921 tctgacgcca tggccagtca gcggtttctt tccacagaat gccccagcaa ggcgataagc
     1981 ttctccttag cgtgtcatca tcttgaaaaa gctcgtgcta cgccggtcgg acaactgcga
     2041 tatgctggcc gctcatcgtc acagaaagct acggacctgg taattctgat tttgatgact
     2101 taccatatcc cttggtaaac gacgggtaag aggaagaaat tcgtcaatgc cttttgaaac
     2161 tccacccctc cgacgagttg attctccatc aatctccgct ttgaagaagg agtaccttat
     2221 gagcctgtac ccatcggctg tcgcaaggat tgtcccactt gggagatccg gactcacaac
     2281 actggggatt cttctcggcc tgctctacat gatgatgacc catgtggccg tcgcacgggc
     2341 agagaacaat ccaaactcgc gggtcatccg gcattctgat gttgtgttca tgtacgataa
     2401 tccagacctt tatgaggctt acggctgcac cgtgctaggt tgggcgggtg cccgatcacc
     2461 ggaaaggatt gccctagctc accagcgagg tgttcgactg ttcgccgtca gtgtcggatt
     2521 tcgcacggaa ttcgcccgca tgatcgattt tacgcccgac tttctgcagg ctgcctgtcg
     2581 taactatgcc ggcgatacgt tcattgtgcc gtggctttgg gatcacaaac acaaaggaca
     2641 acctgcatgg tggttttgca cgaatcatcc cttgtatcgg cagtacctca tgaaacggct
     2701 tgaagagaca gtcgcaagtg gggccgatgc attgcacatt gacgactata caggcacggc
     2761 ttctgcggta acgtggcttg atgcctgttt ctgttcggcc tgcatggcag gatttcgcac
     2821 atatctaaaa acaaaacttt ccgccgagcg gcagaaagaa ctgggaataa ccgatctgga
     2881 cacctttgac taccgccaat ttctcatgaa tcggggaatc actcccgagc gttatcgcaa
     2941 agagcgtccc ggtcttccct tggctacgga gttcctcgat ttccaggtgc aggcggcgac
     3001 cgattttgtg gccgagtacc gacggaaagg cgaagaattg aagggaggcc ctctcccctt
     3061 ggcggtcaac tccggactga caagcccgct cagtctcgcc attacgcccc acgtcagtta
     3121 tttctgttgt gaagttcacc atgcggcgga atctcgtaag gtaccgttcc atcccattta
     3181 tgtttacaaa ctggcggatg gtctcaatcg gcctgtcacc tcgaccgcca gcggtgggga
     3241 ttgggcctac atcatggaga acaatttgcc cggcctcgtg cgaacttggg tagccctttc
     3301 ttacgccttc ggccacaatt tgatggcccc gcaccgacag tggtgttaca cgcagaagaa
     3361 aggaacacac tggtactccg gtcccaccga agaatacgca cctctttatc gctttgtccg
     3421 agaacacgct agtatccttg atgattttga ggccgtggga cgcattgccg ttctttacga
     3481 caatcattcc cggcggctgg gaaaagggga cattgagcgc atcacgatga gcttggccga
     3541 cgcaaattgg ccctttaagg tgctcgtcca agggaatgac tggcttcctg gctatcgcgt
     3601 tgacccaacg acccttggac agttcgataa ggtcatcgtg ccggaagacc tacaaccttt
     3661 gccggagatc caggctatgc tagatcaggc ccgatcccag gggaaactcg tgacgtggtc
     3721 ggcgggcgac aacctggcgc tgctgggaaa aaatcctgta caagtttcca gtcctggagc
     3781 ggtgttcatc gtgccgcgcg tcaagacgac gggtgaacat ccggtcatgg caatccacct
     3841 cgtgaatcgc caatatgatg gagagaagga caagatgatc ccgctgacga atctcaccct
     3901 gaccattgac gaaacagtgc tccccgggcg ggcggatgga tgccgctcgg caaagctcca
     3961 ttcaccgggc aaagaggaca tgaccgttgt ggtcaaaccg gaggcgaaac accttaccgt
     4021 gaccatcccc gagatcgacc tttggaccat tgtcgagctc gagtggcgct gacaagattg
     4081 ggagcgaaac gtataatcaa aaatggctgg tgagcgcggc cgtcacagtg gtcaggttag
     4141 accaagtcgg ccgacgcaag ttgtttgcta tggcagtgtt cgacaacaat ccggggccgc
     4201 gtggccagga tttgttcgga gggtatgcga attggtgagc agccacactg gagatgtggt
     4261 gccccgaaag gggttgcggg tt
//
LOCUS       DAOPTR010000208         4280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16598_length_4280_cov_3.993846, whole genome shotgun sequence.
ACCESSION   DAOPTR010000208 DAOPTR010000000
VERSION     DAOPTR010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16598_length_4280_cov_3.993846"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1731)
                     /locus_tag="PKI05_05285"
     CDS             complement(<1..1731)
                     /locus_tag="PKI05_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOA51648.1"
                     /translation="MRLWDTLRDFSSKYRPSAKGSRKKSEKKPRTHRPRDLRVERMED
                     RVLLSINPATLDRVWQDTLQRAIEQAADLDRYSDAQLQSTTDWVVGIGDDINPAQVAT
                     LLGAQYLPTPTTIKGAYVFRFSSSVDWQTAIQRLQTSELVNYFYPLVPQQRSLCLIPN
                     DPLFVQQWHLLNTGQSGGVPGADLNVVTVWDAYQGDGVVIGIVDDGLDTGHVDLLPNY
                     RSDLSYDYYAGDADPQHELPNDTGHGTAVAGTAAAAGNNATGVAGVAFDAQIAAIRLI
                     SGATSDLQEAQALSHRQDVISIYNNSWGPAGPGDIGFSVFAGPGPLTLAAIQQGVTTG
                     RNGLGNIYVFAAGNDGYLLDNVNYDGFANLRYVIAVGALNENGQQAFYSEPGACLFVA
                     APGGEMADPNDVGSGIITTDWTGPNGSSTGDYTGVQGTSFAAPAVSGVIALMLQANPN
                     LTYRDVQYILAQTARKVDPSDPDWTTNAAGYHINHKYGFGAIDAAAAVQAARNWKPVL
                     SEIALDSGVFQVNQVIPDNSPVGISSTISMASDINRVEWVEVTVDITHLSPGDLEIVL
                     VSPSGTQSVLA"
     gene            2434..2778
                     /locus_tag="PKI05_05290"
     CDS             2434..2778
                     /locus_tag="PKI05_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51649.1"
                     /translation="MKTQVLMWVGATVFLLACGAFLWAAAEPGLIGPRVTQPTTQTPG
                     WIVTAAVGDNRQQIVIADPAQQSLAVYHVDLTTGRIALKSVRHVRYDLLMTEFNTDSP
                     SPRELEALLKSP"
     gene            2840..4009
                     /locus_tag="PKI05_05295"
     CDS             2840..4009
                     /locus_tag="PKI05_05295"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOA51650.1"
                     /translation="MAQKFYTTREAAELLGIAPADLNSMRERNEIRGFRDGMDWKFRA
                     EDIEALRLKLRADATRGAPHQTPSSDEEILLLDDDLKPGDLGTSGSGTVVGGLSGLDA
                     GKSDVKVAGSDVNLAGSGVGISGKPEESSSSVSPFDELAPLETPAETGKSPVGTGSDS
                     AVNLAGEGLEDDDVVIGGSGSGSDITLGSDSGISLMDTNDSGISLEGGLELVPSEESL
                     ELDEDDMLTLAEGADTEAPTQLRTDEEFMLTPFEEAESEETESGSQVIALDTEPATGS
                     SPGMAELLGAEAAAPLGVAPTIPGAVPGAAAPGIGAFPGPVPGVAPVMEPSMVVPVGE
                     APWGTLWNTMLIVCTVLLALCGMMCFDLVRNMWSWNGPYSLNSTIMDAILSVLPK"
BASE COUNT          924 a         1123 c         1278 g          955 t
ORIGIN      
        1 cgcaaggact gattgtgtac cggaaggcga gaccaaaaca atctcaagat cgccaggact
       61 tagatgagtg atatcgaccg tgacttcaac ccattcgacc cggttgatat cggaagccat
      121 gctgatcgtg ctcgaaatac cgaccggact attatccggg atgacctggt tcacctggaa
      181 aacacccgaa tccaaggcga tctctgacag aaccggcttc cagttcctcg ctgcctgcac
      241 ggccgccgct gcgtcgatgg ccccaaatcc gtacttgtgg ttgatgtgat acccggcagc
      301 attcgtcgtc caatcgggat cggagggatc aaccttgcga gctgtctgcg cgaggatata
      361 ttggacatcc cggtatgtaa gattcgggtt tgcctgaagc atcaaggcaa tcacaccgga
      421 aacggccggg gctgcgaaag acgttccttg aacacccgtg taatcgcctg tgctggaccc
      481 attgggaccg gtccaatctg ttgtgataat ccccgacccg acatcattgg gatcagccat
      541 ttccccgccc ggcgcagcga caaacaagca cgccccgggt tccgagtaaa aggcttgttg
      601 accgttctca ttcagtgcac cgacggctat gacgtagcgc aggtttgcaa acccgtcgta
      661 gttaacgttg tccaataggt agccgtcatt accggcagcg aagacgtaga tgttgcccaa
      721 accgtttcgg ccagtggtga caccctgttg aatggcggcg agggtcaagg ggccaggtcc
      781 ggcaaagacc gagaagccaa tgtcgcccgg accagcgggc ccccaactat tgttgtaaat
      841 ggaaataacg tcctggcggt gcgaaagggc ctgcgcctct tgaagatcgc ttgtggcccc
      901 ggatatgaga cggatggccg cgatctgggc gtcaaaagct actcccgcca cgccggtggc
      961 attgttacct gccgcggcgg cagtacccgc caccgcggta ccgtgaccag tgtcgtttgg
     1021 aagttcgtgt tgtggatcgg catcccccgc gtaatagtca tagctcagat cggacctgta
     1081 gttagggagc aaatccacgt gtccggtatc gagaccgtca tcgacgatcc caataacaac
     1141 cccatcaccc tgataagcgt cccagacggt caccacgttt aggtctgcgc ccgggacacc
     1201 accgctttga ccagtgttca acagatgcca ctgttgaaca aacaggggat cgttagggat
     1261 caagcaaagt gaacgctgtt gaggaacaag cgggtagaaa tagttcacca gttctgaagt
     1321 ttgcagccgt tggatggccg tctgccaatc gacactgctc gagaagcgaa aaacgtacgc
     1381 gcctttgatt gttgtgggag tgggcaaata ctgcgcgccg aggagcgttg caacctgtgc
     1441 ggggttgata tcatccccaa tccctaccac ccagtctgtg gtagactgta actgggcatc
     1501 actataccta tcgaggtcgg cggcttgttc gatcgcccgc tggagtgtgt cctgccacac
     1561 ccgatccaaa gtggctgggt tgatgctcaa aaggacacga tcttccattc gctcgacgcg
     1621 caagtcccgg gggcgatgtg tcctcggctt cttttccgat ttcttacgtg aaccctttgc
     1681 gctgggacga tactttgagg agaaatcgcg caaggtgtcc caaagtctca ttgttgaatc
     1741 tccctcgagc aatggatatt tgcggccgac agccgtatca aagacaatcc ctcatttaag
     1801 cgacgatcac gaaactaacg tatctaccga ttaaaattgg ggaacccgca agattcaacc
     1861 caaatcctcc aggtctccca ctttcgcagt cgtttgtcaa ttgcaattca gcactcgacc
     1921 cgtcaagtgg gcagggcggc tgggttgcca cgcttacggg tgcaccgccc ggacacaccg
     1981 gtcggctaag tgctccccat gcgcacgata agcgcacagt tcgtcgtctt tagacgatct
     2041 tgcaaaatcg ttagttccgg aaaaatgggc cggtccgacg ccgtaccact ccggttccta
     2101 tccataccac ccgttagacg tattagtcgt ccagcggctg gattctctgg tacaggtgcc
     2161 gattttctga tctgtcggag ctaaagacgc gcttgggtag gcagagtcgt tggctatgac
     2221 gagcgaacga catggttgac gttcccaact ttcggacgca atcagccatt tctccgcagc
     2281 cggtaaccct ggagagttga gtcggcggcg tggccgggtt aaattaagcg gtgtggaata
     2341 gtgttcccaa attggtggaa tttcgttgac gtgggactgg gtcgttttag aataaatgag
     2401 agagaacgac ccaccggaaa tggatggctt gtcatgaaga cacaggtttt gatgtgggtc
     2461 ggagcgactg tgtttttgct agcgtgcggc gcgtttctct gggctgcggc ggagccgggt
     2521 ttgataggtc ctagggtaac ccagccgacg acacagactc caggctggat cgtcacggcg
     2581 gcagttggtg acaaccgtca gcagatcgta atcgcagatc cagcccaaca gtcgttggcg
     2641 gtgtaccatg tggaccttac aacagggcga attgccctga agagtgttcg ccacgtgcgc
     2701 tatgacctat taatgaccga atttaacacc gattcgcctt ctccgcggga gttggaggcc
     2761 ttactcaagt ctccttagcg atgccaatgg ctggggaacg gtagggggac accaaaccgt
     2821 tatagtgagg tgtgaaacaa tggcccaaaa gttttacacg acgagggaag cagcggaact
     2881 gcttggaatc gctccggcgg atttgaattc gatgcgggag cgaaacgaga ttcgtggttt
     2941 tcgggacggc atggactgga agttccgggc cgaggatatc gaggccctcc gcctgaaatt
     3001 gcgtgccgat gcgacgcgcg gtgctccaca tcagacgcca tccagtgacg aagagattct
     3061 cctcctcgat gacgatctga agcctggaga tctgggaacg agtggttcgg ggacggtggt
     3121 tggaggcctt tcaggtctcg atgctggaaa gagcgatgtc aaggtagcgg gaagtgacgt
     3181 taatctggcc ggaagtggtg ttggaatctc gggcaagccg gaagagtcct cttccagtgt
     3241 ttctcccttt gacgaattgg caccgttgga gactcctgcc gagaccggaa aatccccagt
     3301 gggtaccggc tcggattcgg ccgtgaatct ggccggggag ggtctggaag atgatgatgt
     3361 tgtgatcggc gggagcggca gtggcagcga cattacactc ggcagcgata gcggcatttc
     3421 gctgatggat acgaatgaca gtgggatctc gctggagggt gggttggagc tcgtcccctc
     3481 ggaagagtcg ctggaactgg acgaggacga tatgttgacg ttggcagagg gggctgatac
     3541 cgaagcgccg acgcaactgc ggaccgacga agagtttatg ctaaccccgt ttgaggaggc
     3601 cgaaagcgaa gaaaccgaaa gcggttctca agtcatcgca ctcgacacag aaccggctac
     3661 gggttcctcg cccggcatgg ccgaactctt gggggctgaa gcggcggcac ctttgggtgt
     3721 ggcgccgaca attcctgggg ctgtgcctgg agcggccgcg cccggaattg gcgcatttcc
     3781 cggtccggtc ccgggggtgg ccccagtgat ggagccaagc atggtggttc cggtgggcga
     3841 ggcgccttgg gggacgctgt ggaatacgat gctcattgtg tgcaccgtgt tgctcgcgct
     3901 ttgtggcatg atgtgcttcg accttgtccg caatatgtgg agttggaatg gcccctactc
     3961 cttaaacagt acgatcatgg atgcaattct cagcgtatta ccgaagtgac acccgttagt
     4021 gcgtcttgta cctggcgggc gaggtaaacg caccgttttc cgcggtgcgt tttttgttgc
     4081 atcttgaatg tgatgtccgc ccttgtaacg gcgcgtccaa gcggtgccta gcctttttct
     4141 tgccgctgtc tctataatca gataagtcca ttggcagttc gtgttacagc tatttatgtg
     4201 agttgcacga gggcttggcg gtaagacctg tcaaagttcc tcatggccgg gcaagctttg
     4261 atgttcccct cgggttacgg
//
LOCUS       DAOPTR010000209         4280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16600_length_4280_cov_3.587692, whole genome shotgun sequence.
ACCESSION   DAOPTR010000209 DAOPTR010000000
VERSION     DAOPTR010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16600_length_4280_cov_3.587692"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="PKI05_05300"
     CDS             <1..1011
                     /locus_tag="PKI05_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51651.1"
                     /translation="LLRGTLATPEALASIPPAMLEKYPTLKRFQEALTDAEVKLIELR
                     GQYADEHPAVIAAQLALQDLTNRISEEVPTVIQTIENEQAVAANQKQFLEQKRQTEEA
                     RVQHILAVLPKYRQLMAAIRAQSETVRDAQHRLAIARAGAASARSARLITAVEPPRTG
                     NRPVGPGTLTIVAGGFVCGLFLALCVFLWLTPVAPSETKPIVTSHAPTHEEDGDQPVQ
                     AEMVLPTAKKETRIPSEADVTSHGPLFDSPGAEAQPALASTAIQPTTPMVCGKIARRR
                     QTLPQTGQQSRLPRTGPSHPYTRRSPVWLNPVILGGKHDRLVFGHWCRSKALQTVDNI
                     TG"
     gene            941..2263
                     /locus_tag="PKI05_05305"
     CDS             941..2263
                     /locus_tag="PKI05_05305"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOA51652.1"
                     /translation="MIASSSAIGAVRRPFKLWTTSRADSLLLALVLLTLLAGIWLGHP
                     FWHDDSLGVPITLDRIAWLTVLLGTVFITVRRGVTFTRIARGDLFLAVLVAYLGALAV
                     MQFSSEHTGAAARWVFYYFMPASLYFALRQIHLSSSAERGILLVLGCVGVCLSLTGLF
                     EVFGLRSLIYPAYIMDRSVSGEFLGRARGPLLNPIINGFIMIVAWAAWVFLGRSAPRR
                     WWPGLVLVHGIMAVGVISTLTRSVWLAAGLAGLFLGYHLVPGGYRRAWLATAGFGLIV
                     MALVVKGFFWEMKRDQGLSPAEAARSAQLRPILALVAWEMAKDHPFFGVGLGQYDRAK
                     TKYLANPLASYPVHWAKPYTQHNVFLSILVETGAIGLMVFLFLLAAWTRDMCSAVSQG
                     NLVFPLMAIVAIVAYVTNGLFHDVSIIPQMHTVLFVTLGLAESSADSR"
     gene            2799..3344
                     /locus_tag="PKI05_05310"
     CDS             2799..3344
                     /locus_tag="PKI05_05310"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HOA51653.1"
                     /translation="MPKKPLILDPAEIDTSRIIADIEEIRRYNPQRYEMEQLTAICYE
                     DLERAICAGYKDLGPDEFWVRGHFPGMPIMPGVIMCEAAAQLASYYTIKHKLMKAPMV
                     GFGGLQDVRFRGVVRPGDRFLIVARLLKVRSTMLTCEFQCFVRENLVCEGVILGIPLF
                     PETIEGSRDNGAETASADAGA"
     gene            3563..>4055
                     /gene="trmB"
                     /locus_tag="PKI05_05315"
     CDS             3563..>4055
                     /gene="trmB"
                     /locus_tag="PKI05_05315"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOA51654.1"
                     /translation="MGRRALRKIDPNLDLSFHYRELPNIPKPFQAEVIFGRSAPLEVE
                     VGSGKGLFLRRAAAGTPDVDFVGLEIAQKYARFSAAGLAKLGVRNAIVIHGDAQLFFA
                     EWLLPESVQAVHVYFPDPWWKKRHKKRRVLNETFLKNVQRALRPEGRLHFWTDVAEYF
                     QTTI"
     assembly_gap    4056..4065
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4066..4231
                     /locus_tag="PKI05_05320"
     CDS             <4066..4231
                     /locus_tag="PKI05_05320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOA51655.1"
                     /translation="LLKNTTSLLGPFPVPERPAENDMDFRTHFERRVRLQGGTIYRAE
                     FVKPSSSVLQ"
BASE COUNT          850 a         1226 c         1213 g          981 t
ORIGIN      
        1 ctccttcgcg ggacgttggc cacccctgag gcactggcca gcattccgcc ggcaatgctg
       61 gaaaaatacc ccacactcaa acgctttcaa gaggccctga ctgatgccga ggttaaactc
      121 atcgaactcc gtggccagta tgccgatgag cacccggccg tgattgcggc ccaactggcc
      181 ctccaggatt tgacaaaccg tatatctgag gaagtcccta cggtgatcca aacgatcgag
      241 aacgagcaag ccgtggcggc taaccaaaaa caatttcttg aacaaaaacg ccaaaccgag
      301 gaagccaggg ttcaacacat cttggctgtt ctgcccaagt atcgccaact catggccgcc
      361 atccgcgctc aatcagaaac ggtccgcgac gcccaacaca ggttggccat cgcccgcgcg
      421 ggcgccgcgt cggctcggtc agcacggctt atcacagccg tcgaaccgcc gcgaacgggc
      481 aaccgcccag ttggccctgg gacactcacg atcgtggccg ggggattcgt ctgcgggctg
      541 ttccttgccc tatgtgtctt tctttggtta actcccgtcg cgccttcgga aacaaaaccg
      601 attgtgacat cacatgcgcc aactcacgaa gaagacggtg accaacccgt acaagctgaa
      661 atggtcctac caacggcgaa gaaagagacg cgcattccct ctgaggccga tgtgacatct
      721 catgggcctc tattcgactc tcctggcgcc gaagcccagc cggcactcgc ctcaacggcg
      781 atccagccaa cgacaccgat ggtctgtgga aagatcgcgc gtcgcaggca gacactccca
      841 caaactggcc agcaatcccg gttgccgagg actgggccga gtcaccctta caccaggcgc
      901 tcgccggtat ggctcaatcc cgtgatcctg gggggaaagc atgatcgcct cgtcttcggc
      961 cattggtgcc gttcgaaggc ccttcaaact gtggacaaca tcacgggctg acagcctctt
     1021 gttggccctt gtgctgttga cactcctggc tgggatatgg ttgggacacc ctttttggca
     1081 cgacgacagc ttaggggtcc ccatcacctt ggatcgaatc gcctggttga ctgttctgct
     1141 agggacggtt ttcatcacag ttcgccgggg cgtaactttt acccgaatcg cgcgagggga
     1201 cctcttcctg gccgtgcttg tcgcctatct cggcgcgtta gcggtgatgc aattcagctc
     1261 ggaacacacg ggtgccgctg cccgttgggt cttctattat ttcatgccgg ccagcctcta
     1321 ttttgccctc cgacaaatcc atctatcatc gtcggcggag cggggaatcc tcctggtgct
     1381 tggctgcgta ggggtttgcc tgtccctgac cggattgttt gaggtcttcg ggttgcggtc
     1441 gcttatctat cccgcttaca tcatggatcg ctccgtgagc ggagaattcc taggacgtgc
     1501 acgcggcccc ctgctaaatc ccatcatcaa tggcttcatc atgatagtgg cttgggccgc
     1561 atgggtattt ctggggcggt ccgcccctcg ccgttggtgg cctgggttag tcttggtcca
     1621 cggaatcatg gcagtaggag tcattagcac gctcacccgc agtgtctggc tggccgcggg
     1681 gctcgctggg ctgtttttgg gataccactt ggtgccgggg ggctatcgcc gggcatggct
     1741 ggccaccgcc ggatttggcc taattgttat ggcccttgtc gtgaaaggct tcttttggga
     1801 aatgaagcgc gatcaaggcc tcagccctgc cgaggccgcc cgatccgccc agttgcgccc
     1861 catccttgcc ctagttgcgt gggaaatggc caaggatcat cccttttttg gcgttgggct
     1921 aggacaatac gaccgggcaa agacaaaata cctcgccaat cccttggctt cttaccccgt
     1981 ccattgggca aagccataca cccagcataa cgtgtttctc tcgatcctcg tggaaacagg
     2041 agccatcggc ctaatggtat ttctcttcct tttggcggct tggacccgtg acatgtgctc
     2101 ggcagtgtca cagggaaatc tcgtctttcc attgatggcg atagtcgcga ttgtggctta
     2161 cgtcacaaat ggcctgtttc acgacgtctc gatcatcccg cagatgcaca ccgtcctttt
     2221 cgtaacgctc ggcttggccg aatcctcggc cgacagtcgc taaggcatgg ccagttgtta
     2281 ggcgatcttt cccattttgc ctgttttcac gtcttcacag aaagcccgcg aaaaggtaaa
     2341 ttatcatttt gccccgcttg tggttgccta tggttcgggg tgggccttgt gcctatccat
     2401 aatggcgttt tgtctctcgg atgtgtcagg ccaccatgga atagggccac acggcggaga
     2461 tttcgcgaat agtgatcgat gggatggcct ccgcgggggt gacgctcggg cagaaggcct
     2521 agttttgaaa ggccttttcg gccctgtttc taagtcccat cgtatctgaa atgaggcatt
     2581 gttgtatggc agatgaggtg atctaatggc gaagccgcac cgtcatatta agaaagccaa
     2641 tcacggacgc cgacccgcca actcacgggc gaggcgggcg aagcgctggg tggtaaagac
     2701 ctaggaggtt aggatattca atcgttggtc ttgacaaggg ccatcgggct ggtcctgcgt
     2761 tgtgtgttgc gcggaattgg ttttttgagg agctggttgt gcccaagaag ccattgattc
     2821 tggaccccgc cgaaattgat acgagccgga tcatcgccga cattgaggaa atccgtcgct
     2881 ataacccaca gcgctatgag atggagcaac tcacggcgat ctgctacgaa gatctcgagc
     2941 gggccatctg cgccggatac aaggacttag gcccagacga gttctgggtc cgggggcact
     3001 ttcccggaat gcctatcatg cccggcgtga tcatgtgtga agcggctgcc cagctcgcca
     3061 gctattacac catcaagcac aaactcatga aggcccccat ggtcggattt gggggacttc
     3121 aggacgtccg ttttcgcggc gttgtccgtc ccggggaccg cttcctgatc gtagcccgcc
     3181 tgttgaaagt tcgcagtacg atgctgacgt gcgaattcca gtgctttgtg cgggaaaatc
     3241 tcgtgtgtga aggggtcatc ctgggaattc ccctgtttcc cgagaccatc gagggcagca
     3301 gggataatgg agccgagacg gcctccgcag acgcgggtgc ctagagccta tcccaaaaag
     3361 tctccctctt cctccgggag agggttcggg tgagggcaag cgatcgtcgg agtggatcta
     3421 gaaaaccggc ggcgattcga gcgaccctca cccccggccc atctctcgcc gaacgggcga
     3481 ggggagatta tggaataggc tctcagccct cactgtttcc gtcaccgatc gcgttcctgt
     3541 tagcatattc tccgcggcga ctatgggccg acgagcactt cgtaagatcg atccgaacct
     3601 cgatctctct tttcattatc gggaattgcc gaatatcccc aagccgtttc aggcggaagt
     3661 gatcttcggt cggtcggctc ctcttgaagt tgaggtcgga agtggaaagg gcctgttcct
     3721 ccgtcgtgca gccgctggga ctccggatgt tgacttcgtc ggtttggaga tcgctcagaa
     3781 atacgcccga ttctccgcgg cgggcttggc aaagttgggt gtgcggaatg ctatcgtcat
     3841 ccatggcgac gctcaattat tctttgcgga atggttgctg ccggaaagcg ttcaagcagt
     3901 tcacgtctat tttcccgatc cctggtggaa gaagcgacac aaaaagcggc gcgtgctcaa
     3961 tgaaaccttt ctaaagaatg tgcagcgggc ccttcggccg gagggacgac tccatttctg
     4021 gacggacgtg gcggagtatt tccaaaccac aatcgnnnnn nnnnngctgc taaaaaacac
     4081 cacgtctcta ttggggcctt ttcctgttcc ggaacgcccg gcggaaaacg acatggattt
     4141 tcgcacgcat tttgaacgcc gtgtccgttt acagggcggg accatttacc gggcggagtt
     4201 tgtcaaacca tcttcgtccg ttttgcagta attgaaaccg gccaggcaga agagctcgtc
     4261 cctcgcaatt tgatgcgacg
//
LOCUS       DAOPTR010000210         4275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16630_length_4275_cov_3.557583, whole genome shotgun sequence.
ACCESSION   DAOPTR010000210 DAOPTR010000000
VERSION     DAOPTR010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16630_length_4275_cov_3.557583"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /locus_tag="PKI05_05325"
     CDS             complement(<1..1328)
                     /locus_tag="PKI05_05325"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOA51656.1"
                     /translation="MRFLSGLFAVLICLHTVSLAIEPANPNLCQEGKELLAFFESIYG
                     KQVLAGYNVYPHTPDDYQQTARQAAIWGRDIRWLGDVEEVVSHVRAHGYFLTLHWHWF
                     FDEDSAWTRERKKPVDLAKVITPGTQEHETLMKELAATADVLERLQKEGIVVLWRPLH
                     EIDGGWFWWTNLNHPENTAELWRIMFRYFTDERKLNNLIWVYSAGVGDLKKKPAEWRR
                     RFYPGDAYVDISGIDIYGVDPKNDETPYWDYYKLMEEVSPGKMLALCECDAIPNPDKM
                     AAGKTPKWLYALPWWGCPSPRRSVEWARFTMGHELVLTLDEIPTMGRKDLVPQIGIVE
                     PNDDGSAWFPKSEVTMSGYAVSRGAAVARVEFLANDEVIGTLEKPPAHFSWSWVSPRA
                     GTFDLTARAVDTLGRTNTSNRIHLAVGVTNAAKNKPVRVSSGEDPQAAVDG"
     gene            2122..3684
                     /locus_tag="PKI05_05330"
     CDS             2122..3684
                     /locus_tag="PKI05_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOA51657.1"
                     /translation="MFDCLWSVALLGAVLLTSSYTAFGVEDAQAPAGRLPNIVLILAD
                     DLGYGDVGCYNPESKVPTPHLDKLAAEGMRFTDAHSAATVCTPSRYSLLTGRMCFRTG
                     MRGVFTGAGGPCLIEEGRLTLPQLLRNKGYATACMGKWHVGLTFFDKEGRPIHQDGLE
                     AVKRIDYTRPIPDGPIHRGFDYFFGTACCPGTDWLYAYIEGDRIPVPPTKPLDRSGLP
                     QHPYSEDNRPGLIAPGYNLEELDLVFLEKSRQWLERHVREHPGQPFFLYHATSAVHLP
                     SFPAAQFQGATKAGPHGDFIFELDYVVGQLMQTLERLGVADNTLVIFTSDNGPEVTTV
                     LHMRADYGHDGARPWRGLKRDNWEGGHRVPLIVRWPGRVKPGSICELPICHTDIMATC
                     AQIVGAKLPDNAAEDSNSFLHALLGRSAPEDADRIVLHQTISLALGIRRGAWKYLDHK
                     GSGGNRYDRGALAALAIEDTAPDAPGQLYNLAEDPGERINLAGQKADLAAELKRLLEH
                     FKATGRSAPFHP"
     gene            complement(3846..>4275)
                     /locus_tag="PKI05_05335"
     CDS             complement(3846..>4275)
                     /locus_tag="PKI05_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOA51658.1"
                     /translation="DLVMTENEIRGGRIGWLENLGYGKAWKLYLIPQSDASIRGAYHS
                     LIVADFDLDGDWDVFSCEMEGIMGEKLPRWFVWENVDGKGQNWVEHVVLDAGLGGHEA
                     VAGDFDGDGDLDIISKLWRPRKDNANGGKNHVDLLENLRR"
BASE COUNT          932 a         1305 c         1145 g          893 t
ORIGIN      
        1 ccatccacgg cggcctgtgg atcctcgccg gaggaaaccc gcaccggttt attcttggct
       61 gcattggtca cgcccacggc aaggtgaatg cgattcgagg tgtttgtgcg gcccaaagtg
      121 tccacggccc ttgcggttag gtcaaaagtt cccgcacgcg gacttaccca ggaccacgaa
      181 aaatgggccg gtggcttttc caatgtgccg attacttcgt cattagcgag gaactctaca
      241 cgggccacgg cagcacctcg tgagactgca tagccgctca tagtcacctc gctttttgga
      301 aaccacgcgc tgccgtcatc attaggctct acgatgccaa tttgaggaac aagatctttc
      361 cgccccatcg taggtatttc atcgagagtt aaaaccaact cgtgacccat tgtgaaacgg
      421 gcccattcca cggaacggcg tggggaagga cagccccacc aaggcaaggc gtagagccat
      481 ttcggcgttt taccggcagc catcttgtca ggattgggga tggcgtcgca ctcacaaagg
      541 gccaacatct tcccgggtga aacctcctcc atgagtttgt agtaatccca atagggagtc
      601 tcgtcattct tcgggtccac accatagata tcaattcccg aaatatcgac gtaagcatcc
      661 ccgggataaa atcgccgtcg ccattcggca ggtttcttct tcagatcgcc cactccggca
      721 ctatagaccc aaatgaggtt gttgagcttt cgctcatccg taaagtaacg gaacatgatc
      781 cgccacaatt ccgcggtgtt ctctggatgg ttcaaattcg tccaccagaa ccaacccccg
      841 tcgatctcat gaaggggacg ccacaagacg acgattccct ccttttgaag tcgctccagg
      901 acatcggcgg tggctgccag ttccttcatg agggtctcat gttcctgtgt tcccggggtg
      961 atcacctttg ccaaatccac cggctttttg cgctccctgg tccatgccga atcctcgtcg
     1021 aagaaccagt gccagtgtag cgtcaggaaa tagccatgtg cgcggacatg agatactacc
     1081 tcctccacat cgccaagcca acggatgtca cgcccccaaa tagctgcctg tcttgcggtt
     1141 tgttgatagt cgtccggcgt atgtggataa acgttgtagc ccgccaagac ttgctttccg
     1201 taaatgctct cgaagaacgc aagaagttcc ttaccctctt gacacaagtt aggattggcc
     1261 ggctcaatgg ccaagcttac tgtgtgtaaa caaatcaata cggcgaagag accagacaag
     1321 aatctcatgg caacctcccc atctttgttc tcagtgccga tcgcaattgg ctccacaacg
     1381 ttctgttatc ctatttgacg ctccaatcaa tcgcaatccg ggcatgaacg atttgtggtg
     1441 agcgttcccg gtggatgtac ggcgaccgtg ggtaacaaca gcataccaga aaaccgaccg
     1501 cgccgtcgga aagctgcggg caatgacggg gcctggaagc gtaccgcgga gctgagccta
     1561 ttgcaggtcg agcaggaagc gaaagaccgc cctgtttgac gcaggtggcg atcaccatgc
     1621 agatctgctt acaccaacgg cggtagggga ccatccacag gacgttcgca ctccgcgtcc
     1681 cttgtgtgag ttgcgttgcg ggacggaaaa gagctctccc acgagcttag tcatgccttg
     1741 ggccaaccgg tcatgttcgc agtcggacct cggtgcccac agctttatac ggcggacaac
     1801 taggcatctc cggccgttca accacggccc attgcgtgcc tcccggtatg caataaatgt
     1861 aatcacacaa ggggcttgac aagctaaacc ggccttttca acatcaggac tgccgaccca
     1921 ctggcgtgaa aacaagaatg ggcacgacaa gagtaacacg ccgtgaactg tcacgaaaac
     1981 catgtctaga aggcttgcta tatgtatttg cttaatttag tgcacatgcc tgtttcgctt
     2041 cgcaccatcg cccggttgag cttcccaaat actcattcac cgtgttggct gtggccttca
     2101 cggttgcggg atgggggacg agtgtttgat tgtttgtggt cggtcgcact gctcggtgcg
     2161 gtgctgctga cttcaagtta cacggctttc ggcgtagaag atgcacaagc acctgccggc
     2221 cggctgccta atatcgtttt gatcctggcc gacgacctcg gctatgggga cgtcggttgc
     2281 tacaaccccg agtctaaggt gcccacgccc catctggaca agctggccgc cgagggcatg
     2341 cgcttcaccg acgcccacag tgcggccacg gtctgcacgc ccagtcgcta cagtttgctc
     2401 accgggcgca tgtgtttccg tacgggcatg cggggcgtgt tcactggtgc gggtggtcct
     2461 tgcctgatcg aagaaggccg actgaccttg ccgcagttgc tgcgcaacaa aggctacgcc
     2521 acggcttgca tgggcaaatg gcacgtgggg ctgacgtttt tcgacaagga gggtcggccc
     2581 attcatcaag acgggctgga agcggtcaag cggatcgact atacgcggcc catccccgac
     2641 gggccgatcc accggggctt cgactacttc ttcggcacag cctgttgtcc cggtaccgat
     2701 tggctctatg cctatatcga aggcgatcgc ataccggtgc cccccaccaa gcccttggac
     2761 cgaagcggcc tcccccagca tccctattcc gaggacaatc ggccgggcct gatcgcaccg
     2821 ggctacaacc tggaagaact cgacctggta ttcctggaga aaagccggca gtggctcgaa
     2881 cgtcacgtgc gcgaacatcc cggccagccg tttttcctct atcacgccac cagcgcggtg
     2941 catctgccgt cgtttcccgc cgcccagttc caaggcgcca ccaaggccgg gccgcacggc
     3001 gacttcatct tcgaactgga ctatgtggtg ggccaattga tgcagacgct cgaacggctt
     3061 ggggtggccg acaacacact ggttattttc accagcgaca atgggccgga agtgaccacc
     3121 gtgttgcaca tgcgggccga ctacggccac gatggcgccc gaccctggcg cgggctaaaa
     3181 cgtgacaact gggaaggcgg ccatcgggta ccgctgatcg tgcgatggcc cgggcgggtc
     3241 aaaccgggca gcatctgcga actgcccatc tgccacacgg atatcatggc tacctgtgcc
     3301 cagatcgtcg gggcaaagct gcccgacaac gctgccgaag atagcaacag cttcttgcac
     3361 gccttgctgg gccgttccgc cccagaggat gcggatcgca tcgtgttgca ccagacgatc
     3421 agtctggctc tgggaatccg ccgaggagcg tggaagtatc tcgaccacaa aggctccggt
     3481 ggcaaccgtt atgatcgcgg agccctggca gcactggcca tcgaggacac tgcaccggac
     3541 gcaccgggcc agctttacaa tctggcagaa gatccgggcg aacggatcaa tctggcaggc
     3601 caaaaagcgg acctggccgc cgagttgaag cgcttactgg agcacttcaa ggccacgggc
     3661 cgaagcgcgc cgtttcatcc gtgaacagca aagaattgcc gagaagggta gcgatgacgt
     3721 ccatacccac accaaatgcg atcgctaatg ggctacaaac cgcctggcaa ttgccgagct
     3781 gaaatcccgc cgccagccgc aaacctggtt catcacggta aatcaacacc cctttttcgc
     3841 ggcatttatc gccggaggtt ttccaaaagg tccacgtggt tcttcccgcc gttggcatta
     3901 tccttgcgcg ggcgccaaag cttactgatg atatccaagt ccccgtcgcc atcgaaatcg
     3961 ccggccacgg cctcgtgtcc accaaggccg gcatctagga ccacgtgctc cacccaattt
     4021 tgtcccttac catccacgtt ttcccaaaca aaccaacgag gcaatttctc gcccatgatg
     4081 ccttccattt cgcagctaaa tacatcccag tcaccatcga gatcgaagtc cgccacaatc
     4141 agggaatggt aggccccgcg aatgctcgcg tcactttggg gaatcagata aagcttccat
     4201 gccttgccat atcccaggtt ctccaaccaa ccgatccgtc ctccgcggat ttcattctcg
     4261 gtcatgacca gatcc
//
LOCUS       DAOPTR010000211         4260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16716_length_4260_cov_3.270392, whole genome shotgun sequence.
ACCESSION   DAOPTR010000211 DAOPTR010000000
VERSION     DAOPTR010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16716_length_4260_cov_3.270392"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="PKI05_05340"
     CDS             complement(<1..1205)
                     /locus_tag="PKI05_05340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOA51659.1"
                     /translation="MKSEKAAAASRLPAALSKQVTAEGKGRPSQPHNRFATFFGLAAL
                     CTVLTAIDVAPERGGPGVTCDEFYHISYGKRLVASFHANGLGSFRPSCIESTFAWRKD
                     GPPVHPPLGNWVLGWANWLFDPEPFNPLRVAITPARLGTAICFGCLALIVGLFCAAHF
                     GTISGVIAVVSLVCMPRLFGHAHLAALDLITATMCTATIVAVTWAEQHKRWYSLIVAG
                     VIWGLALLTRFHGLLCLPPVVCYLCYRYRWRAMPLVMLWLTSGLITFFLGWPWLWLDP
                     LGHIQQYLISSTQRIHIHTFYLGRVWNDTVVPWHYPWVMFLVTLPPGLLFLGLAGIIS
                     PFLRARSISETQQKVYNTQPAGIWLVFGTICFFLLVFSLPGVPVYDGVRLFLPVYPLW
                     AILVGWGTLV"
     gene            complement(1202..1780)
                     /gene="dcd"
                     /locus_tag="PKI05_05345"
     CDS             complement(1202..1780)
                     /gene="dcd"
                     /locus_tag="PKI05_05345"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOA51660.1"
                     /translation="MILSGKEILAQLGKNIIIEPFNPKNLNSNSYDLTLHNELMIYEE
                     VVLDMKKPNRVRRITIPEEGLVLTPHQLYLGRTVERTETYGFVPMIEGRSSVGRLGLF
                     VHVTAGFGDVGFRGYWTLEMFAIQPVRVYPGVSICQIFYHEIRGDYEEYRSDKYQNNQ
                     DIQPSLLFKELNPDACRRPKSHLPFGIEESTG"
     gene            complement(1950..2624)
                     /gene="nth"
                     /locus_tag="PKI05_05350"
     CDS             complement(1950..2624)
                     /gene="nth"
                     /locus_tag="PKI05_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOA51661.1"
                     /translation="MPRSPKTIVPKVLEVLKQEYPDASCSLNFSNPLELLVATILSAQ
                     CTDERVNQVTKDLFKKYKTAHDYASVPLEELEKDIQSTGFYKNKAKNLRECCRQIEER
                     FGGRVPKELDQLVSLPGVGRKTANVVIGTAYGIPSGVVVDTHVSRISQRLGLTQNTDP
                     EKIEQDLMKLIPQDEWIDFSHRMIKHGRKYCSARNPSCESCPMTAFCPKIGVEKTGRK
                     KGSANR"
     gene            2908..3318
                     /locus_tag="PKI05_05355"
     CDS             2908..3318
                     /locus_tag="PKI05_05355"
                     /inference="COORDINATES: protein motif:HMM:NF015690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HOA51662.1"
                     /translation="MPDVHVLRGVKAFNAGRFFLAHEIWEDVWRDSLPPAKDYYQALI
                     QAAIAFYHLQRGNFVGARRLADRALGRLEHFDPQYLGLDTRRLRLCLEQVLTGDLTGK
                     KDQINDQTCPSCVPDRSHLPVFIGILDDTIGELD"
     gene            3846..>4260
                     /locus_tag="PKI05_05360"
     CDS             3846..>4260
                     /locus_tag="PKI05_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51663.1"
                     /translation="MWLFVLVISFSSIHLSACGEERSILARIAGTWALCQASSREELL
                     KQQPVLEQALATPHLRGFCLRVPWKAIDQDFLLLGLGRDLARKHNLAFSVRFMAGRHT
                     PSRVFRDGSPHYTHSGSELVPLPFFPDGRPNTIFER"
BASE COUNT         1041 a         1138 c         1128 g          953 t
ORIGIN      
        1 accaacgtgc cccagccaac caggattgcc caaagcggat acacaggcaa aaacaggcga
       61 accccgtcat aaaccgggac cccgggtaaa gaaaagacca gcaagaaaaa gcaaatcgtc
      121 ccaaaaacca gccaaatacc ggcaggttga gtgttgtaca ccttctgctg ggtttcggaa
      181 atggaccgcg ctcttaaaaa gggcgatatt atcccagcga gcccgaggaa aagtaaaccc
      241 ggtggtaacg tgacgagaaa catcacccag ggatagtgcc acggcacgac cgtgtcgttc
      301 cagacccggc cgaggtaaaa ggtgtggatg tgaattcgtt gcgtcgagct gatgaggtac
      361 tgctggatgt ggccgagagg atcgagccat aaccacggcc acccgagaaa gaacgttata
      421 aggcccgagg tcagccaaag cattaccagg ggcatcgctc gccaccgata gcgatagcag
      481 agatagcaga ccaccggcgg taggcacaat agtccgtgga atcgcgtaag tagggcaagt
      541 ccccaaataa cgccagctac tatcaaactg taccaccgtt tgtgctgctc ggcccaagtg
      601 acggccacaa tcgtcgcggt gcacatggtg gccgtgatta aatcgagggc ggcaaggtga
      661 gcatgaccaa acagccgggg catgcatact agcgagacca cggcaatgac gccgctgatt
      721 gtgccgaagt gtgctgcgca aaaaagaccc acgatgaggg ccaaacaccc aaaacagatc
      781 gctgtgccca atcgtgcggg cgtaatggcg actcgtaagg gattgaaggg ctcagggtca
      841 aaaagccagt ttgcccagcc cagcacccag ttgcctaagg gcggatgaac gggtgggcca
      901 tctttacgcc atgcaaaggt actttcaata catgatggcc gaaaagaacc taggccgtta
      961 gcatgaaagg aggccaccaa tcgcttgcca tacgaaatgt gatagaactc atcgcaagta
     1021 acgccaggac caccgcgctc aggagcgaca tcaatcgccg tgaggacggt gcaaagagcc
     1081 gcgaggccaa aaaacgttgc gaagcgattg tggggttgcg atggtctccc tttaccctca
     1141 gctgttacct gtttacttaa cgctgccggt aacctactcg cggcagcagc cttttcggac
     1201 ttcatccggt cgattcctca ataccgaacg gcaaatgaga tttgggtcgg cgacacgcgt
     1261 cgggattcaa ttccttaaag agaaggctcg gctgaatgtc ctggttgttt tggtacttgt
     1321 cgctgcgata ctcctcgtaa tcgccgcgaa tctcgtgata gaaaatctga caaatggata
     1381 cgccgggata gacccgaaca ggctggatag caaacatctc caacgtccaa tacccacgga
     1441 atcccacgtc tccaaaaccc gcggtgacat gcacaaatag cccaagacgc ccaaccgagg
     1501 accggccttc aatcatcggt acaaagccat acgtctcagt tcgttcgact gtcctgccca
     1561 agtaaagctg atggggggtc aatacaaggc cttcctcagg gatggtgatc cgccgtaccc
     1621 gattgggttt cttcatgtct aagacgactt cctcgtaaat cattaactca ttgtggagcg
     1681 tcaggtcata gctgtttgag ttgagatttt tcgggttaaa gggctcgata atgatatttt
     1741 tccccaactg ggcgagaatt tccttgcctg aaaggatcat gctgtctcct tgcttgtgct
     1801 gccaatggcc agtttttgat cagcgtatgg gatttcgttc gaccaacgac gctattttca
     1861 acacgtaagc ataataagcg ctatgataaa tagcggaccc cgctagaaag aaggccccac
     1921 agtccaggca accctttttc gcgtagccct tagcggtttg ccgatccctt ttttcgtcct
     1981 gttttctcca caccgatctt ggggcaaaac gctgtcatgg ggcacgattc gcaagatgga
     2041 ttgcgggccg agcagtactt tcttccgtgc ttaatcatgc gatgactaaa gtcaatccac
     2101 tcgtcctgtg ggatcagttt catgagatcc tgctcaatct tttccggatc ggtgttctgc
     2161 gtaagcccta accgttgact aattcggctc acatgggtat ccaccacaac cccggacgga
     2221 attccatacg cggttccgat cacgacgttg gccgtcttcc gcccgacacc cggcaacgat
     2281 acaagttggt cgagttcttt gggaacccgg ccaccgaacc gttcctcgat ttggcggcag
     2341 cactcccgca gattcttggc cttgttcttg tagaatcctg tgctctggat atccttttcg
     2401 agttcttcca gtggcacgct ggcataatcg tgggccgtct tgtacttctt gaacaagtct
     2461 ttcgtgacct gattgacccg ttcgtctgtg cactgggccg atagaatcgt tgccaccaaa
     2521 agctcaagcg gattgctaaa gtttagggaa caggacgcat cggggtactc ttgcttaagg
     2581 acctcaagaa ccttaggaac aatcgtcttg ggggaacgag gcataacctt gaacctttgc
     2641 ctatgatggt agccattttt tgattgcgag ccgttgctcg gccggggaag gccttctgaa
     2701 aggaatcatt gttgcgtctt ccaacggtac gtcaatcacc caggggagag ccattacgca
     2761 caggcggatg tgccggatga aatcacaaag gcgtttccat tctccgatcg tcccacaccc
     2821 aatttggtag aatggaattc agagcgaagt tgtgaacctc tagtgaaatg cgatcgaccg
     2881 tgtccgcaag tataacggag atacccgttg cccgatgttc acgttctacg cggtgtcaaa
     2941 gcctttaatg ctggtcggtt cttcttagcc catgagattt gggaggacgt ttggcgagat
     3001 tccttgccac cagccaaaga ctactaccaa gccctgattc aggcagcgat agctttctat
     3061 cacctccaga ggggaaattt tgtgggggcg cgccggttag cagaccgtgc cctgggccgc
     3121 cttgaacact tcgatcctca gtatttggga ctcgataccc gccggttgcg attgtgtttg
     3181 gagcaggttc tgaccggcga cctgacgggc aaaaaggacc agataaacga ccagacatgc
     3241 ccctcgtgtg tgccggatcg gtcacacctg cctgtcttta tcggcatact ggacgatacc
     3301 attggagagc tcgattagag ggaagattcc ccgttctttt acatagcaga cggaaagaaa
     3361 cccccaaact aagaccctgc cccaaaaagg ccccccctct ccctccggga gagggttggg
     3421 gtgagggcaa gcgaatgtcg gaattgattg agaaaaccgg cagcgattcg agcccccctc
     3481 acccccgacc cctctcccgc cgaacgggcg aggggagatc atgggatagg ctctaagcac
     3541 aaaaggccaa tggtcgcgtc aacgttggaa agtgaaaggt gacataccgt gaatactcgc
     3601 agccgttaaa acgccagttg tggtaatctc cggaagttaa agtcgatcct ttcgtggcga
     3661 aatggcacaa tgtattacgg gcgccagttg ccccaggtag ataagacgcg aaccggaggg
     3721 agggtaatta ttccatgatc cctatgaaag atgctctgag ccgtcccgcg tcacagaatc
     3781 gtttttcaat tgtcggaaaa ggacggttaa tattagagca ggtatggcca cccaggcgtg
     3841 tgactttgtg gttgttcgtc ctagtaatta gcttttcgtc aatccatcta tcggcctgcg
     3901 gggaggaaag gtcgatcctt gctcgaattg ccgggacctg ggcactttgt caggcctcca
     3961 gccgcgaaga actcctgaag caacaaccag tgcttgagca ggcgttggca acgccacatc
     4021 ttcgaggctt ctgtttgcga gttccctgga aggccatcga tcaagatttt ctactcctgg
     4081 gtttagggcg cgatctcgct cgaaaacata acctcgcgtt ttccgtgcgg tttatggcgg
     4141 gacgtcatac gccaagccgc gtgttccgag atggaagtcc tcactatacg cactccggta
     4201 gtgaattggt ccccttgccg ttttttccag acggtcgtcc caacacgatt tttgaacgtc
//
LOCUS       DAOPTR010000212         4258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16724_length_4258_cov_3.026648, whole genome shotgun sequence.
ACCESSION   DAOPTR010000212 DAOPTR010000000
VERSION     DAOPTR010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16724_length_4258_cov_3.026648"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      47..130
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            398..589
                     /locus_tag="PKI05_05365"
     CDS             398..589
                     /locus_tag="PKI05_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HOA51664.1"
                     /translation="MRSLALKLQRFLVSEDGPTAVEYAVMLALIVVVCLTAISSLGTS
                     ANSTFQSVSDSIQSTAGGG"
     gene            942..1520
                     /locus_tag="PKI05_05370"
     CDS             942..1520
                     /locus_tag="PKI05_05370"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HOA51665.1"
                     /translation="MLERLTETADQPWMIWAVCWFVSVILVVAAWIDGRKLKVPNWLT
                     FPLILSGWAASGYFFGWSGLYSSWLGTAVGLGLLLPAYAIGGMGAGDVKLLAGVGAWI
                     GMAHTFWAFVLTVVIGAILAVAMVAARGAWGKHIRQFFAILFEILIVRDPEKLSALAA
                     ERKPKMLLLPYGIPIAIGTIVYFLVNGMLFPV"
     gene            1613..2065
                     /locus_tag="PKI05_05375"
     CDS             1613..2065
                     /locus_tag="PKI05_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HOA51666.1"
                     /translation="MGETSQARARSRRWLRCSPRTPRAGVAVVEFALVAPLFFLLVLG
                     MIEFGRMVMVQQVLVNASREGARAGIVQGATNTDVLAVVQSYLEGGGIHGATVNVSPD
                     PIDLAGYGDPVTVAVSVPFRQVSWLPTPIFLGDTELRATTTMRREAIQ"
     gene            2110..3192
                     /gene="cpaB"
                     /locus_tag="PKI05_05380"
     CDS             2110..3192
                     /gene="cpaB"
                     /locus_tag="PKI05_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HOA51667.1"
                     /translation="MRTKSVLLLLLALGCGLVAAIGVKQVLATKDQATASPTVEMQAI
                     VVAAEDIAMGDPVKIEQIKLEDWPKSKIPEGALFKAEEAEGRRTKTKIYKGSPILDDQ
                     LLAKGAVDAGAAPWIPKGYRVVSVKVDAVSGSASMLRPSDRVDVVVHFPANPNKGIPK
                     DYTRTILQNVRVFAVDDKYILTEDEKTKKSLPAKTVSLLVTPEQAEVITAASELGQIR
                     LVMRGLGDNDPVNTRGVDPSELLGRPSMGGDPEKEVLPSETPSGSADEFAQFLVSQQK
                     PGPQVMRVSSSSSENRETWSIRLLMGPRVSDVTLERESGDPNEERGTLWRVVSAEGMS
                     DAPPQVDLPLPELPPEELPPPRGSDQ"
     gene            3349..>4258
                     /locus_tag="PKI05_05385"
     CDS             3349..>4258
                     /locus_tag="PKI05_05385"
                     /inference="COORDINATES: protein motif:HMM:NF025014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HOA51668.1"
                     /translation="MIRLKGRKRLLLGLAITLALVAALPATGEVAPGPPEPSSPNQVV
                     RQITGPSQREEMTVNTSRILTLDKRIPQAQVNNPDILDITALSPTQIQIFAKKAGVTQ
                     VNLWDEKGNIYTIDVIVFGDARELDLLLKSQFPNCSIKVIPIASGVLLSGYVDQQEHV
                     SRIIQIAEEYYPRVINNLTLGGVHEVLLHVKVMEVSRTKLRALGFDWTQISGQNVIRS
                     SVSGLISAANAANPAAGTAATAVTSGGETFFFSVFDGNDAFFGVLEALRQDNLMKVLA
                     EPTLVTVSGRPAFFHVRGAFPTLAPPS"
BASE COUNT          904 a         1088 c         1288 g          978 t
ORIGIN      
        1 gtggaatggc cactgagtcc cctgtcagaa aggcctgtcg cctcatcctc gaaaaaggca
       61 aaccggtcgt aaggccgggg cgcaaagcta cgggccgggt tggtgacaaa acggtcgccg
      121 ggctgccgaa agggtgatgc agtccagggt gatcctggac atccgcctgc cgacaaccgg
      181 gacatgccgg ctgcggcggc gcgcatccgg aaaaccgggt gagcatcgcg gcatacgggg
      241 aagaggcatt aggttttggt tgttgggatg cggacggtac gggacaggcc gacgccacag
      301 gtatgtggcg tgtaccgtcg gtcccccttt tccgactggg tttgcggaag gacgcagaca
      361 ccagtcaaac atgtcccgct tgtcttggag gaactgtatg agatcgctcg ctttgaagtt
      421 gcaacgtttc ctcgtttcgg aagatggccc gaccgctgtt gagtacgcgg tgatgctggc
      481 tctgatcgtg gttgtgtgct tgacagcgat ttcctcgctc ggcacatccg ccaacagcac
      541 cttccagagt gtcagtgaca gcatccagag cacagccggt ggcggttgat gatgggtaac
      601 ttagccccgc tgtgttaagc gggctgtccg gcgggattca gtgaccctcg gaatcgaaaa
      661 atcgattccg agggcttttt ttgacctaga cttgtattag aagagcatga cggtccgtta
      721 gggttttcgt actaagtgtt gttagggctg aaataatggg aggtaccacc atggatccgc
      781 ttgtgccccc ggaactcgct ggacacgcat tttcgctgct ggccctgtgg ctaacgttgc
      841 tggccaccgt tttgcagtac atgtttacgg cacgtttgta aacaaacaac actcaggagt
      901 tttgatcttc gcctaacaat cttcatggag ccgaaccaac tatgttagag cgacttacgg
      961 aaaccgcaga tcaaccgtgg atgatttggg cagtgtgttg gtttgtcagc gtcatcctgg
     1021 ttgtggcagc ctggattgat ggtcggaagt tgaaggtgcc aaactggctg acgttccctc
     1081 tgatcctaag cggttgggcg gcgagcggct atttcttcgg atggtcggga ttgtactcca
     1141 gttggctggg cacagccgtg ggtttggggc tcctcctgcc cgcctatgcc atcggcggca
     1201 tgggtgccgg cgatgtcaag ctgctagccg gtgtcggggc ctggatcggc atggctcaca
     1261 cattttgggc gtttgtcctg acggtggtga ttggggccat ccttgcggtc gccatggttg
     1321 cggctcgggg ggcttggggc aaacatattc ggcagttctt cgctatcctt tttgagatat
     1381 taattgtccg ggatcccgaa aagctttcag cccttgctgc ggaaagaaaa cccaaaatgc
     1441 tcctattacc gtacggaatc ccgatcgcta ttgggaccat tgtgtacttt ctcgtcaacg
     1501 gcatgctttt cccggtctaa accggttgcg gtcatggcaa atggagaggt gttctatgag
     1561 attcgccagc ttgtctaagt ctcctgctct aacaaattgt caagatcgcg gaatgggaga
     1621 gacctcacaa gcgcgcgctc ggtcccgccg ttggttgcgg tgcagtcccc gaacacctcg
     1681 ggcgggggtg gccgttgtcg agtttgcgct ggttgcaccg ctgtttttcc ttcttgtctt
     1741 agggatgatc gagtttggac gtatggtgat ggtccagcag gtgttggtga acgcctcgcg
     1801 ggagggtgcc cgcgctggca tcgttcaagg cgcaacaaac acggatgtcc tcgcggtcgt
     1861 tcagagctac ctagaaggag gcggtattca cggggcaaca gttaacgtta gtcccgatcc
     1921 gattgacctt gcgggttatg gtgatccggt aacggttgcg gtatcggtgc catttcgtca
     1981 agtcagttgg ctaccgacgc caattttttt aggggacacc gaattgcgcg cgacaacgac
     2041 gatgcggcgg gaagccatcc aataagccgt ttatcagaaa acacattttg actgaggttt
     2101 ttgccaatta tgcggacaaa atcagtccta ttgctattac ttgccctagg ttgcggactt
     2161 gtcgctgcta tcggcgtcaa acaggttttg gcgaccaagg atcaggctac ggcatcccca
     2221 acggttgaaa tgcaggcgat tgtggtggcg gctgaagaca ttgccatggg ggatcccgtc
     2281 aagattgagc agatcaaact cgaggattgg ccaaagtcga agattcctga gggggcatta
     2341 tttaaggccg aggaggctga aggtcgccgc acaaagacca agatctacaa gggttctccc
     2401 attttggatg atcagcttct cgccaaaggc gcggtggatg ctggcgccgc tccatggatt
     2461 cccaagggtt atcgggttgt ctcggtcaag gtggatgccg tcagcggaag tgccagcatg
     2521 ttgcgtccta gcgaccgggt tgacgtggtt gtgcacttcc ctgccaaccc caataagggc
     2581 atcccaaagg actacacgcg gaccatcctc cagaacgtgc gggtcttcgc cgtggacgac
     2641 aagtacatcc tgacagaaga cgaaaaaact aagaaatcct tgccggcaaa aacggtttcc
     2701 ctcttggtaa cgcccgaaca ggccgaggtc attacagcgg cgagtgaact gggccaaatc
     2761 cgcctggtaa tgcgcggctt gggggataat gatccggtta atacgcgcgg ggttgacccc
     2821 agcgaactat tgggcaggcc gagtatgggt ggagacccgg agaaggaggt tttgccgagc
     2881 gaaactcctt cgggttctgc cgacgaattt gcgcaatttt tggtgtccca gcaaaagccg
     2941 gggcctcagg tcatgcgggt aagtagcagt agtagcgaaa acagggaaac ttggtcaata
     3001 cgactgctga tggggccgcg ggtgagcgat gttactctgg agcgagaatc cggcgatccg
     3061 aacgaggaga gggggacgct ttggcgggtt gtgagtgccg aagggatgag tgatgctcct
     3121 ccccaggtcg atttgcccct tcccgagttg ccgccggaag aattaccgcc tccccgaggg
     3181 agtgatcagt aaagttaaaa tacaaaaaag ccgttaagta ttcttagagg gcaagcgcac
     3241 cagcgcggga ggctgttgtt gggtgcgaca aggatgtcgt gtccgacaat ctaaaaccgg
     3301 ccgcgatgga ttcttgttgg cggagccttg ttttgaggac gatcacggat gatcagactt
     3361 aaaggacgga aacggctttt gctaggtctg gctataactc ttgccctggt ggccgctctt
     3421 ccggcgacgg gagaggtcgc gccgggaccg ccggaaccct cgtctcccaa ccaggtcgtt
     3481 cgtcaaatta ccggacctag tcagcgggag gaaatgacgg ttaacacaag ccgcattctc
     3541 acgctagata agcggattcc ccaggcacag gtcaacaatc ctgatattct ggacatcaca
     3601 gccctttctc cgacacaaat ccaaatcttt gccaaaaaag cgggcgtgac ccaggtgaat
     3661 ctctgggatg aaaaaggaaa catttacacc atcgacgtca tcgttttcgg cgatgctagg
     3721 gagttggacc tcctactgaa aagccaattt cctaattgca gtatcaaggt cattcccatt
     3781 gccagcggag ttttgctttc gggatacgtc gatcagcagg agcacgttag ccgaatcatc
     3841 cagattgccg aggaatatta cccgcgtgtc atcaataacc taaccttggg tggggtccac
     3901 gaggtcctcc tccacgtcaa agtgatggag gtctcgcgga caaaattgcg tgctttgggg
     3961 ttcgactgga cgcaaatctc cggtcagaat gtgatccgct cgagcgttag tggtctgatt
     4021 tcggcggcta acgcggccaa ccctgccgcc ggcacggcag ccacggctgt gacgagtggc
     4081 ggcgagacgt ttttcttcag cgttttcgat ggaaacgacg ccttctttgg ggtcttggag
     4141 gccttgcgac aggacaactt gatgaaagtc ctagccgaac cgacactggt gaccgtcagc
     4201 ggccgaccgg cgttcttcca cgtccgtggc gcgttcccca ccctcgcccc gcccagct
//
LOCUS       DAOPTR010000213         4253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16744_length_4253_cov_3.614102, whole genome shotgun sequence.
ACCESSION   DAOPTR010000213 DAOPTR010000000
VERSION     DAOPTR010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16744_length_4253_cov_3.614102"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..3399)
                     /locus_tag="PKI05_05390"
     CDS             complement(136..3399)
                     /locus_tag="PKI05_05390"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOA51669.1"
                     /translation="MRPSTQRTATRWGFNGRLIQALCAKVLPDMWYEVVLGAVVLTAL
                     CLGGCHRTAYRRQADMEVSTLLQQGQRDPRWRLRDTTVYPKKESRYYDPFCPDAEPMP
                     PDDPVSHQLMHYVDGKKGWPHWHDNGDTYYVESPFWRAYLPRDERGVVVLDRARAVDL
                     ALVHSREYQRQIENLYLSALNVTAERFRFDVQFFGGHSTFFTSNGSKRAGGPRSLLEL
                     DTDAQARKLGATGTELLVGFANSLVWQFSGSDNYASASLLNFSLVQPLLRAGGRAVIL
                     ESLTQAERNLLANIRQMAHYQRGFYLQVVTGTNPGAGPSAGGLSIGSLTPSVTPGRGG
                     VLGLLQTQVQIRNQEQNVQGLEDSYEQLLAAHQADRIDRYQVDLALQSLYNAQSQLLS
                     ARSSYQSQLDNFKVSLGLPPQLPVEISDDFLTQFDFVDPRLLNLQRRTTLLVRALRDP
                     ATPNPPDWLDQMRSIYEQTARQVEIVENDIARLTAVLPKRKTLLREFYSKLVTEDGQL
                     EGLTIADKAIADLDARYQAIIADFGLLLEGKLPAARGEERVPRQPLKQLLENLQQFIQ
                     DPDSVTNKRVSELEAQLAELQNQAKVADEELNKLSQTMATAPQEEVLALQAKYSEVRA
                     QSEILRSRIDSITRTLTELKASPKEQVAMQLLEPLLLQLSDLSLIQARARLDSVTLTP
                     VDITPEEALEIARENRLDWMNARAALVDQWRQIQIAANRLRSDLDVTFSGDLGTTTDN
                     PFSFDGNTGQLRIGFRFDAPFTRLVERNQYRSALIAFQRARRDYIAFEDQIAQSLRET
                     LRNIRLGQNTFELRRSAVLVSIAQVDLARERLRAPPRPGETSQFGVNTARDLVTALSG
                     LLSAQNAFLGAWVEYEVQRMVLDFQMGTMQLDDRGHWIDPGPIRSQNRGPGAEAVPAP
                     APQTEVRPLPTITANPTDSLNQGRFASGEEFKNLDSDENGTAISPVLPSGLTGASQGV
                     PVGESRNSGSQPHLSPALQRLKDRLASYQKSPDGFEERVFPPAASGGSVTVGQVGVGN
                     KASPAVADFRKGEPDFGTIQTTFAPTENGLPESLLPSIQSLLDLENEGVAVISDRPE"
     gene            3839..>4253
                     /locus_tag="PKI05_05395"
     CDS             3839..>4253
                     /locus_tag="PKI05_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51670.1"
                     /translation="MSIWNKILICLILLASLAMWYLGMRALATHRAWRTAAKRLQSEL
                     AQLEAERERLIHGDGTTKGIRQLQVELAKYTSQRGRVWNGCRPVRVDVRQDPQAGLVV
                     QVSVVVDRLGLAGLQEVQELPVDSLVYLFDERDIQQ"
BASE COUNT          918 a         1141 c         1241 g          953 t
ORIGIN      
        1 cggttacaag cctaagacac tgattggcat tccgtttgac attcaaccca cgccggtcga
       61 ggtccctaaa cgatcatttg ctaccgacta tgtccggcgt tgagcttttt ccgaccctcg
      121 ttgcggatgc aagcgtcatt caggtcgatc cgaaatcacg gcgacgcctt cgttttccaa
      181 gtctagcagt gactggatgg atggcaacaa ggattcaggt aaaccgtttt ccgtcggcgc
      241 gaatgttgtt tgaatcgtgc cgaagtcggg ttcacctttc cggaaatcag caaccgccgg
      301 gctagcctta ttgccgaccc caacctgccc caccgtgaca cttcctccgg aggctgcggg
      361 tgggaaaact ctttcctcga aaccgtccgg cgatttctgg tacgacgcca aacgatcctt
      421 gaggcgttgc agcgctgggg agagatgcgg ttgagaaccg ctgttacgac tctcccccac
      481 cgggacgcct tgagaagctc cagtgagacc gctaggtaaa accggagaaa tggccgtacc
      541 attctcgtcc gaatccaggt tcttaaactc ctcgcctgat gcgaagcgac cctgattaag
      601 actatccgtt ggattggctg tgatcgttgg gagaggacgc acctccgttt gaggtgccgg
      661 cgccggaacc gcctcggcgc ccggtcccct gttttgggaa cgaatcggac cggggtcgat
      721 ccaatggccg cgatcgtcga gttgcatggt ccccatctgg aagtcgagga ccatccgctg
      781 cacttcatat tccacccaag ccccgagaaa ggcattctgg gcactgagaa gtccggaaag
      841 agccgtcacc aggtcgcggg ccgtgtttac accgaattgt gaggtctccc ccggtcgcgg
      901 cggagcacgc aagcgctctc gggcaaggtc cacttgggca atggagacaa gcactgcgga
      961 acggcgcagc tcaaacgtgt tttgtcccaa ccggatattc cgtagggtct cccgcaagct
     1021 ctgggcaatc tggtcctcaa aggcgatgta atctcgccgt gcccgctgaa atgcgatgag
     1081 ggccgatcga tattgattcc tttcgaccag acgagtgaac ggtgcatcaa aacggaaacc
     1141 gattctcaat tgtcccgtgt tgccatcaaa gctaaacggg ttgtcggttg tcgtgcccaa
     1201 gtctccacta aaggtcacat ccaaatcact gcggagccga ttggcggcaa tctggatttg
     1261 acgccattgg tcaaccaaag ctgcacgggc attcatccaa tcgaggcggt tttccctcgc
     1321 aatttctaag gcttcttccg gtgtgatatc aaccggagtc agcgtcacag aatccaaccg
     1381 cgcccgggcc tgaatcaggg agaggtccga caactgaagt aaaagtggct caagcaattg
     1441 catcgccact tgctccttgg gcgacgcctt aagttccgtg agagttcgag tgatggaatc
     1501 gatacgggag cgaagaatct ccgactgggc tcgaacctca gaatatttcg cttgcaacgc
     1561 aaggacttcc tcctggggcg ctgttgccat cgtttgcgag agcttgttca gttcctcgtc
     1621 cgctaccttg gcctggttct gtaactcggc cagttgggcc tctagttcgc tgaccctctt
     1681 gtttgtcacg gaatcggggt cttggatgaa ttgctgtaaa ttctccaaca gttgtttgag
     1741 tggttgccga gggactcgct cttccccacg tgcggccggc agtttccctt cgagaagaag
     1801 accaaagtcg gcgatgatgg cctgatagcg ggcgtccaag tctgcgatag ccttgtctgc
     1861 gatcgttagc ccttccaact gtccgtcctc cgttaccagt ttcgagtaaa actcgcggag
     1921 gagggttttc cgcttgggaa ggacggccgt gagacgggca atgtcgtttt cgacgatctc
     1981 cacctggcgt gccgtttgtt cgtagatcga ccgcatctga tcgagccaat ccggcgggtt
     2041 tggggtggca gggtcacgca acgcccggac tagcaaagta gtccgccttt gcaggttgag
     2101 tagtcgtgga tcaacaaagt cgaactgagt tagaaaatcg tctgagattt ccacgggcaa
     2161 ttgcggcgga agacctaaac tgaccttgaa gttgtcgagc tgagactgat aggacgaccg
     2221 ggcggacaat aattggcttt gggcgttata aagggactgc agggccaggt ccacttgata
     2281 tcggtcgatg cggtcggcct gatgggccgc aagcagttgc tcgtagctat cttccaatcc
     2341 ttgaacgttc tgttcctggt tccggatttg aacttgtgtt tgcagcagcc ctaagacacc
     2401 accccgccca ggagtgactg acggggtgag cgagccgatg ctcaggcctc ccgcgcttgg
     2461 accagcgcca ggatttgttc ccgtgacgac ttgaagataa aaccccctct gataatgtgc
     2521 catctgacga atgttggcca ggaggttgcg ctccgcctgc gtcaggcttt ctaggataac
     2581 ggccctcccg cccgcacgga gcaatggttg aaccagcgaa aagttaagta atgaagccga
     2641 agcgtagttg tcgcttccgg agaattgcca aacgagggaa ttggcgaaac cgacgagtag
     2701 ctcggtcccc gtcgcaccca gttttcttgc ctgagcatct gtgtcaagtt ccaggaggct
     2761 tcgtggtccc ccggcacgct tgctaccgtt actcgtgaaa aaggtggaat gccccccgaa
     2821 gaattgaaca tcaaaccgaa accgctcggc cgtcacattc aaagccgaca gatagagatt
     2881 ttcgatttga cgctggtatt ccctggaatg gacgagtgcc aaatcgacgg cgcgggcgcg
     2941 gtcaagcaca acaacgcccc gctcgtcccg gggaagataa gcccgccaga acggactttc
     3001 aacatagtag gtgtcaccgt tgtcgtgcca atgcggccaa ccctttttgc catccacgta
     3061 atgcatcaat tggtgcgaaa ccggatcgtc gggaggcatg ggctcggcat cagggcagaa
     3121 ggggtcgtag taccgggatt cctttttggg atagaccgtt gtatcgcgga gtcgccaccg
     3181 tgggtcgcgc tgcccctgct ggagaagggt actcacttcc atgtcggcct ggcggcggta
     3241 ggctgtgcga tggcagccgc ccagacatag tgcggttagg acaacggcgc caagaaccac
     3301 ctcgtaccac atgtcgggaa gcactttagc gcagagggct tggatcagtc tgccattgaa
     3361 accccacctt gtcgctgttc tttgggtcga tggtcgcatt ctttgacctc gttgacacaa
     3421 acccggaaac ggcctcaaag caagaaggtc acccattaat cgttcggccc ataaaatgcg
     3481 taaacttaag caattgtctg agatttgccg aaagtaacgg ctttgccggc cagatacctc
     3541 tcaagtgggg ggtagcgcga aaacgattgc gaagaaatgg ttaaagcttg acaggggcag
     3601 gggggctaaa taccatcgaa cgtaacggat gggattgtgg agaaagtatc cccggcaggt
     3661 gcgaagttgt ggatgagtgc ccggggaaac ggcctggggt gtgggcttgg tttgttaaaa
     3721 aatcatgagc gatcgcggtt aggaggttcg gtaggctgga gagggtgtac cagggacggg
     3781 taacggcttt gggacatctg gaacttctta ggaatctccg ttcgctggtg gaaccacaat
     3841 gagcatttgg aataagattc tcatctgcct gattttgctc gcgtccctgg cgatgtggta
     3901 cttggggatg cgcgccttgg ctacccaccg tgcctggcga accgccgcca aacgattgca
     3961 atcagagctt gcccaactcg aagcggaacg tgaacgattg attcacggcg atggcacgac
     4021 aaagggaatt cgccagctcc aggtagagtt ggccaagtac acatctcagc gtggccgcgt
     4081 ttggaacggt tgccggccgg ttcgggtgga cgttcggcag gatccccaag ccgggcttgt
     4141 ggttcaagtg agtgtggtcg tcgaccgact cgggctagct ggtttgcagg aggttcaaga
     4201 gttgcccgtc gattcgctcg tctatctttt cgacgagcgt gatattcaac aag
//
LOCUS       DAOPTR010000214         4249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16762_length_4249_cov_3.672628, whole genome shotgun sequence.
ACCESSION   DAOPTR010000214 DAOPTR010000000
VERSION     DAOPTR010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16762_length_4249_cov_3.672628"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="PKI05_05400"
     CDS             <1..679
                     /locus_tag="PKI05_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51671.1"
                     /translation="PRTNAGYWAQLLAADTHLQIGCRQILVNKADGAQELRKALDGYL
                     AVRNVTISPLFQQRVLWGLGRAYEALSGTREGQGELDRAMTAYRELVQRWPDGPFSSI
                     AKRRLESLEKSETREFYDALAAYEPVRPSVQPPSGIIDFGPSSLPTPPVQPSSGDETG
                     AAPASDTKPGEQVVQEKSETSPPSEPTVEMPLPDVNAPISETDQGQGEEKASEPKADS
                     GEDKLDS"
     gene            839..1837
                     /locus_tag="PKI05_05405"
     CDS             839..1837
                     /locus_tag="PKI05_05405"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008663759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOA51672.1"
                     /translation="MVVDSVLGENPIELIVTPHEADWRLDAFLVYHFPAYSRSLLRQV
                     ITAGGVTVDDKGAKPAYRLKVGQKVVVKLPELPRQCPKPENIPVEVLYEDDWLAVINK
                     PPGMVVHPARGHWSGTLASAIQYLYGGKLSTLGGPNRPGIVHRLDADTSGAIVIAKHD
                     LAHGKLASQFAERTVDKTYLAIVSGVPDRDADWIDEPIGLHPRQREKMAIRRYDPEAR
                     HAQTLYRVVERFRGFALVSAHPKTGRTHQIRVHLAHIGCPVLCDRYYGGRASLTRGEI
                     RGDPADETVILDRQALHAWKIAFTHPGTNERLEVVAPLPADMAGVLEELRQYRQAT"
     gene            complement(1860..2636)
                     /locus_tag="PKI05_05410"
     CDS             complement(1860..2636)
                     /locus_tag="PKI05_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOA51673.1"
                     /translation="MSSTRRIWDRRESTDDSLDVLVVGAHPDDVEIGCGGFVHALARK
                     GLRVGIVDLTDGEPTPECPHPDQRLDEAIKAAETLGVQLRVTLDLPNRRLFDDFESRI
                     ALAKEIRRYRPRIVLGLGGQTPMASPDHQQAAQIIEAAVFYARLTKWDELFENLAPHT
                     VGVYLHYYLAMRYPILPTPNAVVVDIGECLSVKLEAIGRYASQFAHRPEILNRIKTYN
                     QAIGQSVGFQAGEFIGHPTGWGFRDLIGGILGEGPRGPVE"
     gene            complement(2742..4013)
                     /gene="cbiE"
                     /locus_tag="PKI05_05415"
     CDS             complement(2742..4013)
                     /gene="cbiE"
                     /locus_tag="PKI05_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HOA51674.1"
                     /translation="MANNARKIYVVGIGEDGFAGLTDYARQLIQSADLVVGEPHCLEL
                     IGEIAGQRVPVSSDLDEVVKTITENRDKQIVILALGDPLFYGIARYLWDKIGKEAFEV
                     VPHVSIMQMAFARVKESWEDALLVNLAHTSLEDLFNQIRSAETVGLFTTEELPPNVIA
                     QQLLSTGLDYFMAYVCENLGAPNERVTFGELADITKHTYSPLNVLILVRKPDVPDRPL
                     SLFGKRFFGNPDEAFLQSKPKRGLLTPREIRCIVLAELDVGPSSIVWDVGAGSGAVSV
                     EAAQLAREGHVYAIEMDPEDVRLIQENARRFGVTNLTPVLGRAPEAWQGLPNPDCVFI
                     GGSGRQVVGLCEAAYSRLQPGGRIVLTMGSLENVVDVHAFLRRHTKCLNVLMVNLARG
                     FYQFDRVRFQSLAPTFVVSAIKVKENSPKAG"
BASE COUNT          992 a         1170 c         1196 g          891 t
ORIGIN      
        1 tccccgcacg aacgctggtt actgggctca actccttgcg gcagataccc atctccaaat
       61 cggttgccgc cagattctcg tcaataaagc cgacggggca caggaactcc gtaaggccct
      121 ggacggttac ctggctgtac ggaatgtcac aatttctccc ttattccaac agcgggtatt
      181 gtggggactt ggccgggcct acgaggccct atccggaaca agggagggcc agggagaatt
      241 ggatcgggcc atgactgcct atcgcgagct agtccaacgt tggcctgacg gtccatttag
      301 cagcattgct aaacggcgat tagagtcgtt ggaaaagtcg gaaacacgcg agttttacga
      361 cgccttggcg gcctatgagc cggtgaggcc aagcgtgcaa ccgccatcgg ggatcatcga
      421 ctttggccca agcagtctcc ccacgccccc tgtgcagccg agtagcggtg acgagaccgg
      481 agcggccccc gcctcggata caaagccggg cgagcaggtg gtccaggaga agagcgaaac
      541 atcgccacca tcggagccga ccgttgagat gccgctgccg gatgtcaatg cgccgatatc
      601 cgaaaccgac cagggacagg gcgaggaaaa ggcctcggaa ccgaaagcgg attcgggcga
      661 agataagtta gactcttagg gagaggcgac accgtaagtt gtccaatccg tcgcgacaaa
      721 gctgttatcg gaaggcattg aaccgcggta tgatggcggc gtactcgtcg gagttgggag
      781 agggcctggc tgcgaacccg cttggggctg tcgtaataac aacaggtttt accatcgcat
      841 ggtcgtggac agcgtattgg gagaaaatcc gattgaactg atagtcactc cccatgaagc
      901 cgactggcgt ctggatgctt ttcttgtcta tcactttccg gcatatagcc gtagcctcct
      961 tagacaggtc attacggcag gtggggttac cgttgacgat aagggggcca aaccggcgta
     1021 tcggctgaaa gtcggccaaa aggtagtggt caagctccca gagcttcctc ggcaatgccc
     1081 caaaccggag aacataccgg tcgaggtgct ttacgaagac gattggttag ccgttatcaa
     1141 taaaccgccg gggatggttg tccatccggc gcgtggacac tggtcgggaa cgctggcgag
     1201 cgccatccag tatctctatg ggggcaagtt aagtactctt ggtggcccaa atcgaccagg
     1261 gatcgtccat cgtcttgacg ccgataccag tggtgccatt gtcattgcta agcatgattt
     1321 ggcacacggt aagctcgcca gtcaatttgc tgagcgcact gtggataaga cctatcttgc
     1381 gatcgtctcc ggggttcccg accgtgatgc cgattggatt gacgagccga taggcctcca
     1441 cccacggcag cgggagaaga tggccattcg gcgttatgac cccgaggctc gtcacgccca
     1501 aacgctgtac cgggttgtgg aacgattccg aggtttcgct ctcgtttctg cccatccgaa
     1561 gacggggaga actcatcaga ttcgtgtcca tctcgcacat atcggttgtc ccgtactgtg
     1621 tgatcgctac tatggtgggc gtgcttctct aacacgtgga gaaatccgtg gcgatccggc
     1681 agatgagaca gtgatcctgg accgacaggc ccttcacgca tggaaaatcg ccttcacgca
     1741 tccgggcacg aatgaacgtt tagaagtcgt tgcgccgctt cccgccgata tggctggagt
     1801 tctcgaggaa ttaagacagt atcggcaagc gacatgacat tacggacgaa gggaactgac
     1861 tattcgaccg gaccccgtgg accttcgccc aagatgcccc caatcaaatc gcgaaaaccc
     1921 catccggtcg ggtgcccaat gaactcccct gcttgaaatc ccacgctttg gccaatcgcc
     1981 tggttgtaag tctttatgcg atttagtatt tcgggacgat gggcgaattg tgaagcatac
     2041 cggccgattg cttccaactt cacgctgagg cactcaccga tgtccacaac gactgcattc
     2101 ggcgttggta gaatgggata acgcatggca agatagtagt gcaaatagac cccgacagta
     2161 tgtggggcga gattctcgaa tagttcatcc catttcgtaa gccgggcata gaaaacggcc
     2221 gcctcaatga tttgggcggc ttgctggtga tctggggacg ccatgggggt ctgcccacca
     2281 aggccgagga caatgcgggg acggtatctg cggatctctt ttgccagggc tatgcgggat
     2341 tcaaagtcgt cgaatagccg tcggttgggc aaatcaaggg tgacgcggag ctggacgcca
     2401 agagtctccg ccgccttgat cgcctcatcc aaccgttgat cggggtgggg acattcgggc
     2461 gtcggctctc catccgtaag gtccacaatc cctacccgaa gccccttcct ggccagggcg
     2521 tgaacgaatc cgccgcaacc gatttcaaca tcatccgggt gggcaccgac aaccagcaca
     2581 tccagagaat catcagttga ttcacgtcgg tcccaaattc ggcgtgtcga actcatagat
     2641 acctcgtcga agtcaaacga cgaaatgaca caagtgacag actcacgacg caaaaaagat
     2701 agaagaccac cgtgcccgaa tgcgaaatct catccgaggc cttatcctgc cttgggcgag
     2761 ttctccttca ctttgatcgc agaaacgaca aaggttggag caagtgattg gaagcgaacg
     2821 cggtcaaatt gatagaatcc tcgcgcgaga ttgaccatga gcacattgag gcacttcgtg
     2881 tgtcgccgca aaaaggcgtg gacatcgacc acgttttcca ggcttcccat cgtcaagacg
     2941 attcgcccac caggttgcaa acggctgtag gcggcctcac acaggccgac gacctgacga
     3001 ccacttcccc cgatgaagac acaatcggga ttcggaaggc cctgccaggc ctctggagcg
     3061 cgacccaaga ccggcgtgag attggtcacc ccaaatcgcc gggcattttc ttgaatgagt
     3121 cttacgtcct cgggatccat ttcgatggcg taaacgtgtc cctcgcgggc aagctgggcg
     3181 gcttccacac tgacggcacc gctacccgcg ccgacgtccc aaacgatgct cgacggtcct
     3241 acgtctagct ccgccaggac aatgcagcga atctctcgcg gagtcaaaag cccccgtttc
     3301 ggtttcgact gcaaaaaggc ctcgtctggg ttgccaaaaa atcgcttgcc aaaaagactt
     3361 aacggtcgat ccgggacatc aggttttcgc accaagatca agacattaag cggcgagtat
     3421 gtgtgtttag tgatgtcagc cagctctccg aaagtgaccc gttcgttcgg cgctcccagg
     3481 ttttcgcaca cgtaggccat aaagtagtcc aagcccgttg acaagagttg ctgggcaatc
     3541 acgttggggg gcagctcctc tgtcgtaaaa agaccgaccg tctccgcgct tctgatctgg
     3601 ttgaacagat cttccagcga agtatgggca aggttcacaa gaagggcgtc ttcccagctt
     3661 tctttgacgc gggcaaacgc catttgcata atgctcacat gagggacgac ctcgaaagct
     3721 tcctttccaa tcttgtccca caggtaccgt gcgatgccgt aaaacaaggg gtctcccaag
     3781 gccagaatga cgatctgttt gtcccggttt tccgtgattg tcttaacaac ctcgtcgagg
     3841 tcggaagaga cagggacgcg ttgtccagcg atctcgccaa taagctccag acaatgtggc
     3901 tcgccgacta cgaggtctgc ggattgaatg agctgacggg cgtagtctgt caaaccagca
     3961 aaaccgtctt ccccaattcc cacaacgtag attttacgag cattgttggc catcgtaagt
     4021 aaacactccc atgctgcgat tggaggtgcc gatattccta atccgtgatt atggtaaagc
     4081 ctatcttaaa cggtgagcta gggaaagatg gagcgagtgc caaacgctcc tctcagaaca
     4141 acggctgatg gtcttggtgg gaattaacat tgtggagtgt cgcaggctta ggccgacgta
     4201 tcttaccgga ggttggccca atctaaaagg aagcgcatcc ctacggtgg
//
LOCUS       DAOPTR010000215         4246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16779_length_4246_cov_3.424242, whole genome shotgun sequence.
ACCESSION   DAOPTR010000215 DAOPTR010000000
VERSION     DAOPTR010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16779_length_4246_cov_3.424242"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="PKI05_05420"
     CDS             <1..1160
                     /locus_tag="PKI05_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51675.1"
                     /translation="RWSATLILLGVATAFHVLVGGWSLVATALAWLFEGKRRPPIARL
                     FPAVAVAVILALPGLIPSLALTWGVPAAIIREANRIYTFERLAHHLVPGSFRPQDVAK
                     FLLLTLAFLGLAFLPRNDDGWKRFRHFVLGSLVIAGCGWAIGLTVNIFPDLAAALLRY
                     YWFRLSDVTIPLGTAFVAGFLASHRISLPLPWRDTCLAVVFVFLAYHLGNLVAIRARH
                     GIPRTEWSITMLSAWDEDAAARDEFEDWREVCLWIAQNTPTDSRFLTPSMKSSFKWYA
                     QRSEVATWKEIPQDARSIVAWWEKLKDIYIFHDPLWGSWWVRNAARLGEDRLLTLAAK
                     YQFEYILTVSEPRLNLEIVFAKPDNMFVVYRARRMSQGLSHHGQPSNRNSP"
     gene            complement(1133..2425)
                     /gene="tyrS"
                     /locus_tag="PKI05_05425"
     CDS             complement(1133..2425)
                     /gene="tyrS"
                     /locus_tag="PKI05_05425"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOA51676.1"
                     /translation="MPEINVYEELRWRGLIHQTTDDAVLPGFLMERSRTVYAGFDPTA
                     DSLHVGHLMALMILRRFQKAGHRPIALVGGATGMIGDPSGKSEERRLLSEEELRKNVE
                     AVEKQLKRFLDFEGKETSALLLNNYDWMGKFGYLEFLREVGKHVPVNVMLTKESVKTR
                     LERSDSGLSYTEFSYMLLQAYDFVHLYREYGCEIQVGGSDQWGNITAGIDLARRLIGV
                     QLYGITCPLLTKSDGTKMGKTESGALWLDPQKTSPYQFYQYWINVEDDLVEKCLKFFT
                     DLSREEIASIVAQHREAPENRLGQRRLAQEITRLVHGESGLQAAERATAILFGAEIEQ
                     ISERELDEIFSDVPSRTVSRSQLEGPGLPIVDALCLADLAKTKGEARRTIQQGGAYVN
                     NRRVTDQEVRLTAENLVGDRTIVLRVGKKRYGLLRFEG"
     gene            complement(2546..>4246)
                     /gene="glgP"
                     /locus_tag="PKI05_05430"
     CDS             complement(2546..>4246)
                     /gene="glgP"
                     /locus_tag="PKI05_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOA51677.1"
                     /translation="GYFKQHLTVDGWQEEEYIDVDVTKLPLDLCTDAEGKPIIITLET
                     RSSRLLVKVWQLRVGRVNIYLLDTDVEGNSPEDRELTSRLYGGDNRTRIRQELVAGVG
                     GVRALRALGIKPGVYHLNEGHSAFAVLEVMRERMVDDGLSFEEARREVRQCTVFTTHT
                     PVPAGHDRFDPGLMEEHLGPLRDALGISHETLMSLGRVDPTRMDEPFCMTVLALKVSR
                     KANAVSALHGRVTRQMWANLYSWRAEDEVPIGHITNGVHIPSWLAWQMKILYDRYFPE
                     RWYERMGEPEVWQSIYNVDPGELWETHLTLKNQLMAFVRRRLSRQCRRRNEPDEVIEA
                     ARSILDPNALTIGFGRRFATYKRATLILRDPDRLARIVNDPERPVQFIFAGKAHPRDI
                     AGKEYIRAIANLRHDPRFNKRIVFIEDYDINVCRHMIQGVDVWLNTPRRPLEASGTSG
                     QKAILNGALHMSVLDGWWAEAYDGSNGFAIGNGSSHKSEENTDARDAESLYQVLENQV
                     IPEFYDQDSDGLPRRWVLRMMNSISSLAWRFSSHRMVADYVRLAYLPAACGTSSEMSR
                     "
BASE COUNT          880 a         1306 c         1104 g          956 t
ORIGIN      
        1 gtcgctggtc ggcaacactc attctccttg gtgtggcgac agcgtttcac gtgcttgtgg
       61 ggggatggtc tttggtagcc acggcgttgg cctggctgtt cgaaggtaag cgacgccccc
      121 caattgccag gctttttcca gccgtcgctg tggcagtgat cctggctctt cccggtctga
      181 ttccctcgct ggcgctgacg tggggtgtgc ccgccgcgat catacgagag gccaaccgca
      241 tatacacctt tgagcggctt gcccatcacc tcgttcccgg gtctttccgt ccccaagatg
      301 tggcgaagtt cctattgctt acgctcgcgt ttctgggctt ggcgttcctt ccacgtaacg
      361 acgacggttg gaagcgcttt cgccattttg ttctgggatc actcgtcatc gccgggtgtg
      421 gctgggccat tggtttgacg gtgaacatct ttcccgatct ggcggcagcc ttgttgcggt
      481 attattggtt ccgcctgtcc gatgtgacga tacctttggg gacggcattt gttgcgggct
      541 ttttggcctc gcaccgcatt tcgctaccac tcccttggcg ggatacctgt ctggccgtcg
      601 tgttcgtgtt cctggcgtat cacctgggta atctcgtggc tatccgcgca cggcacggca
      661 tcccgcgcac cgaatggtcc atcaccatgc tatctgcctg ggatgaagat gcggcagccc
      721 gggacgagtt tgaagactgg cgagaggtct gcctatggat tgcccagaat accccaaccg
      781 attcgcgctt cctaacacca tccatgaaga gcagcttcaa atggtatgcc caacggagcg
      841 aagtggctac ctggaaagag attccccagg acgcccggtc cattgtggcc tggtgggaaa
      901 agctcaagga catctacatc ttccacgacc cgctgtgggg gtcctggtgg gtgagaaatg
      961 ccgcccggct gggcgaagac cgcctcttga ctctagccgc caaatatcag tttgaataca
     1021 tcctcaccgt atcagaacca cggctaaatc tggaaattgt gttcgccaag ccagacaaca
     1081 tgttcgttgt ctatcgagcg cggcggatgt cacagggcct ttcacaccac ggtcaacctt
     1141 cgaaccgcaa cagtccatag cgttttttac cgacccgcag aacgatggtt cggtccccaa
     1201 ccaggttttc agcagtcaga cgaacctctt ggtccgtgac acgccggtta ttcacgtagg
     1261 cgccgccctg ctgaatggtg cgtcgagctt caccttttgt cttggccagg tctgccaggc
     1321 aaagggcatc gactatgggc aagccagggc cttccaattg cgagcgcgaa accgtccgac
     1381 tggggacatc actgaaaatc tcgtctaact cccgctcgga aatctgctcg atctcggccc
     1441 caaacaagat cgccgtggcc ctttctgccg cttgcaggcc ggattcgccg tgcaccaatc
     1501 gggtgatctc ctgagccaag cgacgctgtc cgagacgatt ctccggggct tcacggtgtt
     1561 gagccacaat ggacgcaatc tcctcccgag acaagtccgt gaagaatttc aggcactttt
     1621 cgaccaggtc gtcctccacg ttgatccaat actgatagaa ctgataagga cttgtcttct
     1681 gcggatcaag ccatagcgcc ccgctttccg tcttgcccat ctttgtccca tcgctcttag
     1741 tgaggagggg acaggtgatc ccataaagct gaacaccgat gagccgccgc gcgagatcga
     1801 tccccgccgt aatattcccc cattgatcgc tcccacccac ctggatttcg cacccatatt
     1861 cccgatacag atgcacgaaa tcataggcct gcaggagcat gtaactgaat tccgtgtaac
     1921 tcagaccgga gtcgcttcgt tctagccggg ttttcaccga ttccttggtc aacattacgt
     1981 tgacgggaac atgcttgccc acttcccgaa gaaactcgag atagccaaat ttgcccatcc
     2041 aatcgtagtt attgaggagg agggcggatg tctctttacc ctcgaagtcg agaaacctct
     2101 tgagctgttt ttcgaccgcc tccacattct ttcttagttc ctcctcggaa agaagccttc
     2161 gctcttcgct ctttccactg ggatcgccga tcatgcccgt ggcgcctccc acaagggcaa
     2221 tcggccggtg acctgccttt tgaaatctcc ttaagatcat caaggccatc aagtggccta
     2281 catgcagact gtctgccgtg ggatcaaacc cggcgtaaac tgtgcgggac cgttccatca
     2341 aaaagccggg caacacggca tcatcggtcg tctgatggat caacccccgc cagcgaagtt
     2401 cctcataaac gttgatctca ggcatggctt aagtgaaagg atctatgcaa aaacttagga
     2461 aaggaccgcc gcggcgtgtg agcaacgtct gctacccgcg agaaccttcg atcgacctgc
     2521 ctccgccgac aacggcccaa agccatcaac gcgacatttc cgagcttgtc ccgcaggccg
     2581 ccggcaagta ggccaaccga acataatcgg ccaccatccg gtgagagcta aatcgccagg
     2641 ccaaggaact gatggagttc atcatgcgaa gcacccagcg gcgtggcaag ccatcactgt
     2701 cttggtcgta aaactccgga atgacctggt tttccaagac ctgatacagg ctctcggcat
     2761 ctctggcgtc ggtgttctcc tcgcttttat gactcgaccc gttaccaatg gcgaagccgt
     2821 tggaaccgtc gtaggcttcg gcccaccaac catcaagaac cgacatgtga agggccccgt
     2881 taaggatggc cttctgccca ctggttccac tggcctccaa ggggcgccgc ggcgtgttta
     2941 gccagacgtc caccccctga atcatatggc gacagacgtt aatgtcgtaa tcctcaataa
     3001 acacgattcg cttgttgaac cggggatcgt gtcgcaagtt tgcaatggcc cgaatgtatt
     3061 ctttcccggc gatgtcccgc ggatgggcct ttccagcgaa aatgaactgc acaggtcgtt
     3121 cgggatcatt gacgatccgc gccagtcgat cgggatcccg caagatgagc gtcgcccgct
     3181 tgtatgtggc gaaccgccgc ccgaagccaa tcgtcagtgc attgggatca agaatggacc
     3241 gcgcggcctc gataacctcg tcgggttcat tccgccgccg gcattgtcgg ctgagccgtc
     3301 gccggacaaa ggccatcagt tgattcttca gcgtcaaatg cgtttcccac aattcgcccg
     3361 gatcgacatt ataaattgac tgccagacct cgggttcacc catgcgttca taccaacgct
     3421 cggggaagta tcgatcgtag aggattttca tttgccaggc caaccaactg gggatgtgca
     3481 caccgttggt gatatggcca atgggaactt cgtcttccgc ccgccaggaa taaaggttgg
     3541 cccacatttg ccgtgtcacc cgaccgtgaa gggcactgac ggcattcgcc ttgcgtgaca
     3601 ccttcagggc cagcaccgtc atgcaaaacg gctcatccat gcgggtggga tcgactcgtc
     3661 ccaggctcat caacgtctca tgggagattc ccaaggcatc tcgcaacgga ccgaggtgtt
     3721 cctccatcag tcccggatca aaccggtcgt gacccgccgg aacaggcgtg tgcgtggtaa
     3781 acacggtgca ctggcggacc tcccgacggg cttcttcaaa gctcaacccg tcgtccacca
     3841 tccgttcacg catgacctcg agcacggcaa aggcgctgtg gccttcattc aggtgataaa
     3901 ctcccggctt aatccccagc gcccgtaacg cacgaacccc cccgacacct gccaccaatt
     3961 cctggcgaat ccgggtccgg ttgtcaccac cataaagccg actggtcaac tcacggtctt
     4021 ccggactatt tccctcgaca tccgtatcca agagatagat attgacccgc cccacccgga
     4081 gctgccaaac cttaactagt aaccgagagc tccgcgtttc tagggtgatg atgatcggct
     4141 taccctcggc atccgtgcac agatcgagtg gcaacttggt cacatcgaca tcgatgtact
     4201 cctcctcctg ccagccgtcc accgtcaaat gttgcttgaa ataccc
//
LOCUS       DAOPTR010000216         4238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16822_length_4238_cov_3.771456, whole genome shotgun sequence.
ACCESSION   DAOPTR010000216 DAOPTR010000000
VERSION     DAOPTR010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16822_length_4238_cov_3.771456"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3843)
                     /gene="hrpA"
                     /locus_tag="PKI05_05435"
     CDS             complement(<1..3843)
                     /gene="hrpA"
                     /locus_tag="PKI05_05435"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HOA51678.1"
                     /translation="MLLTGRELSRLERQIDQAMRADRFRLGRWLRRLCTRARDRRLSD
                     EEVRRFEEELEASVTLRRKRLTERPYVEILPNLPIFAKRDEIAEAIRANPVVIVCGET
                     GSGKSTQLPKICLQIGRGIEGLIGHTQPRRIAARSIATRIAQELGVTLGREVGYKVRF
                     SDVTSPETYIKLMTDGVLLAETQSDRFLEHYDTIIIDEAHERSLNIDFLLGYLHQLLR
                     RRRDLKVIITSATLDAERFAQHFALGQRPAPIIEVPGRSYPVEVRYRPPLPDESTGEI
                     DWQSAVVAAMEAVLAEGPGDILIFMPTERHIHETMRLLKARASGDPRFRDIELLPLYA
                     RLSATEQQRIFQPHKQRRVVIATNVAESSLTVPGIKYVIDPGVARLSRYSPRTKTQRL
                     PIEPISQASAEQRKGRCGREGPGLCIRLFSEEDLLVRDRYTPPEILRTNLASVVLQLM
                     ALNLGQVETFPFLDAPRADAIRDGYKTLFEIGAVDEEGKLTPLGRRLAKLPVDPRIGR
                     MILAAHEEGCLHEVLIIAAALEVDDPRLRPAERAEEADLAHAAFRHPDSDFLGYLKLW
                     DFYQNLKEKLSRNQLQKACQQNFLSYNRLREWADIFRELQEIVTESGMKITKRRNDAA
                     AIHRAILTGLLSNVAMRHNRYEYTVASGGKAYLWPGSGVFQSKPPWVVAAELVETGRR
                     YLRICAKIDPAWVEVLAPHLIKKSYVDPYWSRRYGAAMVYEKVTLFGLPVVPRRRTPL
                     GPVDPQTAREMLIQHGLVEGEIHCDAAFFVHNQRLVREMETAQVKLRRVDLLLGQWAQ
                     FDFYDQRIPSHVYDLDGLLHWWNEASKSDPNILHMRPSDVVREEPPPDIDKQFPDHLL
                     VEKAPLPLTYCFQPGQPNDGVTVTVPLELFHCLDPRRLEWLVPGMLESKIAALIKSLP
                     KEKRRRLIPVQETARQTLQMLSFGQGRFLPAVAEVLSRLAGETIRPEDFQLDKIPPEL
                     HMSIQVVDAEGKVVAKGRNWTELREKLGTRQAVAFSAIDDPHWNRDGLTDWDFDLLPE
                     EIEVRRGDIPVKAFPMLVDAGNTVSLRLADSPARAAHQSRFGIRRLLAIAVLPHLDPR
                     WDEFPDCDRLRLMAATLPGFDFQNQLLLALIDRAFLDESLVGPWQIGEWGNLPRTRAE
                     YRRLFRMGLKRLPLAVQEVLSLIRPLLETYHQAAVALQNAESVQWEESRADIQEQLAE
                     LTCPGFLTHTPWNWLRQYPRYFRGISRRIEALRLGGVFRDREAMAVFRPYWEAFLQRR
                     QFHQELDIFDPELVLYRWM"
BASE COUNT          925 a         1205 c         1234 g          874 t
ORIGIN      
        1 catccaccgg taaaggacga gttctggatc gaagatatcc aactcctgat ggaattgacg
       61 gcgctggagg aaggcctccc aatacgggcg gaaaacggcc atcgcttcac gatcgcgaaa
      121 gaccccgccc agccgcaagg cctcaatccg tcgggagatg ccgcggaagt atcgggggta
      181 ctgtcgtagc cagttccaag gtgtgtgcgt cagaaagccg ggacaggtca attccgccag
      241 ttgttcttgg atgtccgccc gagattcctc ccattgcacg gactcggcat tttgtaacgc
      301 gaccgccgct tggtggtagg tttccaaaag gggacgaatc aaggaaagga cttcctgcac
      361 ggccagggga agtcgtttga gccccatccg aaacaggcgg cggtactctg cccgtgtccg
      421 gggcagattt ccccactcgc cgatctgcca cggtcccacg aggctttcgt ccaaaaaggc
      481 acgatcgatg agcgccagca ggagttgatt ctgaaagtcg aaacctggta acgtggccgc
      541 cataaggcgc aggcgatcac agtccgggaa ttcatcccac cgtggatcga gatggggtaa
      601 gacggcaatg gcaaggaggc gtcgaattcc aaaccgcgac tggtgggcgg cccgggcggg
      661 gctgtccgcc aaacggagcg aaaccgtatt tcccgcgtcc accaacatag ggaaggcctt
      721 gacgggaata tcgccacggc ggacttcgat ctcctccggc aaaagatcaa agtcccaatc
      781 ggtcagtcca tcccgattcc aatggggatc gtcaatggca gaaaatgcca ccgcctgtct
      841 ggtccccaac ttttcgcgga gttccgtcca gtttcgtccc ttggcgacca ccttaccctc
      901 ggcatcgaca acctggatcg acatgtgcaa ttccggggga attttgtcca actgaaaatc
      961 ctcgggacga atagtctccc cggccaagcg ggataaaact tcggccactg ccggaaggaa
     1021 tcgaccttgg ccgaaagaca gcatctgaag ggtctggcgg gcggtctctt gcaccgggat
     1081 gagtcgccgc cgtttttcct ttggcaacga cttgatgaga gcagcgatct tgctttccag
     1141 catgcccggc acaagccatt ccaagcgacg gggatctaag cagtggaaga gctccagcgg
     1201 caccgtcacg gtgaccccgt cgttgggttg tcctggctga aaacagtatg tcagcggcag
     1261 cggagccttt tccaccaaga gatggtcggg aaactgcttg tcgatgtcgg gcgggggttc
     1321 ttcgcggacc acatcgcttg gccgcatgtg caagatgttg ggatcgctct ttgacgcctc
     1381 gttccaccaa tgaagaaggc cgtccaaatc atacacatgg gaggggatcc gctgatcgta
     1441 aaaatcgaac tgggcccatt gtcccaatag gagatcgact cgccgcagtt ttacctgagc
     1501 ggtttccatc tcgcgaacga gccgctgatt gtggacaaag aaggccgcat cgcaatggat
     1561 ttccccttca accaggccgt gctggatgag catttcgcgg gcagtttgcg gatcgaccgg
     1621 tcccaaaggc gttcgacgtc gcggcaccac cggcaaacca aagagcgtca ctttttcata
     1681 gaccatggcc gccccataac ggcgactcca atacgggtcc acgtaggact ttttgataag
     1741 atggggtgcc aatacctcga cccacgccgg gtcaatcttg gcacatatcc gcaggtagcg
     1801 ccgccccgtc tcgaccaact cggcagcgac aacccagggt ggtttgctct gaaacacgcc
     1861 cgaccctggc caaagatacg ccttgccccc gctcgccaca gtgtattcgt accgattgtg
     1921 acgcatggcg acgttagaaa gaagccccgt caagatggcg cggtggattg ccgctgcgtc
     1981 gttccgtcgc ttggtgattt tcataccgga ctcggtcacg atttcctgga gttcccggaa
     2041 gatatcggcc cactcccgca accggttata cgacaaaaag ttctgttggc acgccttctg
     2101 aagctggttg cgcgacagct tttccttcag gttttgatag aaatcccaca gtttcagata
     2161 accgaggaaa tccgagtccg gatggcggaa cgcggcgtga gccaggtcgg cctcctcagc
     2221 cctttccgcg ggccgcaacc gtgggtcatc aacttccagg gctgccgcaa tgatcaagac
     2281 ctcgtgaaga cacccttcct cgtgtgcggc taaaatcatc cgcccgatgc gaggatctac
     2341 aggaagcttg gccaaccggc gtcctagcgg tgtcagtttt ccttcctcgt caacggcccc
     2401 gatttcaaaa agcgttttgt acccatcgcg gatggcatcc gcgcgggggg catccaaaaa
     2461 gggaaaggtc tcgacttggc caagattcaa ggccatcagt tgcaggacga ccgatgcgag
     2521 gttggttcgg agaatttccg gcggggtgta gcggtcgcgg accaacaggt cctcttcgct
     2581 gaaaaggcgg atacaaagcc ccggcccctc ccgaccgcaa cggccttttc gctgctccgc
     2641 cgaggcctgt gagatcggct cgatcggcaa acgttgggtc ttggtgcgag gagaataccg
     2701 actcagccgg gcaactccgg ggtcaatgac gtacttgatc ccggggaccg tgagagagga
     2761 ttccgctacg ttggtcgcga tgacaacccg ccgttgtttg tggggctgaa agatccgctg
     2821 ctgttccgtg gccgaaagac gcgcgtagag cggcagtagc tcgatatctc ggaaccgcgg
     2881 atcgccgctc gcccgggctt tcaaaagacg cattgtttcg tggatgtgcc gctccgtggg
     2941 catgaagatg aggatgtcac caggcccttc agcgaggacc gcctccatgg ctgccaccac
     3001 ggcagattgc cagtcgattt cccctgtgga ttcgtcggga agtggcgggc gataacgaac
     3061 ctccaccgga taagaccgac caggcacctc gataatcgga gcaggtcgct gtcccagggc
     3121 gaaatgctgg gcaaagcgct cggcatccag tgtggccgac gtgataatga cctttaaatc
     3181 tcgacgccgc cggagcaact gatgcaaata gcccaggaga aagtcgatat tgagcgatcg
     3241 ctcatgggct tcgtcaatga tgatcgtgtc ataatgttcg agaaaacggt cactctgggt
     3301 ctccgccagc aagaccccgt ccgtcattag tttgatgtag gtttcgggcg aggtgacgtc
     3361 agaaaaacgg accttgtagc cgacctcccg gcctagcgtg acaccaagct cctgggcgat
     3421 acgggtggcg atggatcggg cggcaattcg ccggggctgt gtgtggccga tcaagccttc
     3481 aatcccacgc ccaatttgga ggcagatctt tggcaactgg gtggatttac cggagccggt
     3541 ttcgccacag acgatgacca ccggatttgc ccgaatcgcc tcggcaatct catcacgttt
     3601 tgcaaaaatc ggcaaattgg ggaggatttc gacataaggc cgctcggtga gtcgttttcg
     3661 gcggagggtg accgaggcct cgagttcttc ctcgaatcgc cgcacttcct catcggaaag
     3721 ccgacgatcg cgcgcacgcg tacaaaggcg acgcagccac cgtcccaagc ggaaacggtc
     3781 ggcacgcatt gcctgatcga tttgacgttc cagtcggctt aactcgcggc cggtcaacaa
     3841 caacctttcg ctccctttta ttgactgtcg gctccgtacc aacgtaaaag tctgggcatt
     3901 tttcgccatg gcgccattcg gcgccatgat ggccaattgt cgaaagccct tttttcaaac
     3961 tgtatcggtt cattcgttaa agggaaatac atggccgata agggaaatga tgagcatttc
     4021 gaataaacgt gcggcatgac gttgtctatc attgcctatt gagaaatttt ttggataaaa
     4081 tgtcaaagga gtttgctagc tgcgtcctgt cggttgtgga ccggtcatcg ttccaacccg
     4141 aggggaccta ggaaatcaag agtgcgtgcc gggaaggagt agggaggacg tcctttaacg
     4201 actgcggcga aggccggctg ggcctacatt gtggcgct
//
LOCUS       DAOPTR010000217         4229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_16868_length_4229_cov_3.311931, whole genome shotgun sequence.
ACCESSION   DAOPTR010000217 DAOPTR010000000
VERSION     DAOPTR010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16868_length_4229_cov_3.311931"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..692
                     /locus_tag="PKI05_05440"
     CDS             126..692
                     /locus_tag="PKI05_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51679.1"
                     /translation="MHIRDRIKELRRIPARQLRPNPRNWRVHPAKQQHALRGILAEIG
                     YADALLARELPDGTLELIDGHLRAETTPDAIVPVLVLDVSEAEANKLLALLDPLAGLA
                     EQDHDALAQLARDIEFESPVLQQLLAETTQAADNPPQESPPETETSAVDPVPELFQVL
                     VECRDEDDQRRLFERLSAEGYTCRLLTL"
     gene            737..1909
                     /locus_tag="PKI05_05445"
     CDS             737..1909
                     /locus_tag="PKI05_05445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOA51680.1"
                     /translation="MKKHVVEVVLETPLDRSFRVMQVAGMFDLPLEKVTRERIAVEIP
                     QQTEDWQLGLIVGPSGSGKSTLARLLFGDRVSRTFTWPDDQAIVDAFGQWPIKEIVGL
                     LTAVGFSSPPAWLRPYRVLSNGEQARCDLARAFAEALQSGPVEDAQPWHVLLPPTADR
                     SRPLVVCDEFTSVVDRHVARAIAAGLAKRLRKGTLPCRFVAVTCHYDVADWLTPDWVI
                     DMATREFSRRSFRRPEITLAVVPCRREAWRAYRRHHYLSGSLPSAAESYLALWDGEAV
                     AFCAVASQIGRRHYRRISRLVVLPEYQGMGIGSAFLEAMARYYRKRGQRVGITTSHPA
                     MIAHLARSPQWRLRSLRKWGSRGGGRNLPQYRGSFGRAVMSFEFIGHASYRQPTAA"
     gene            1979..2473
                     /locus_tag="PKI05_05450"
     CDS             1979..2473
                     /locus_tag="PKI05_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51681.1"
                     /translation="MTETQGQETARRGLDEFRKREILAILSVGCSRRTAARYVGCSPS
                     TIRRTAERDPAFAEALHKAESKAQILFMQNIAAAARKEQYWRAAAWALERLNPEDYAA
                     RSPHAVTLEQIRTLVSEFARIIVEEVPIAAHRKKILRRLERLGREWDVPPSVAVNRTD
                     PSDE"
     gene            2487..4109
                     /locus_tag="PKI05_05455"
     CDS             2487..4109
                     /locus_tag="PKI05_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51682.1"
                     /translation="MPRNTFAESDLVLNQPQAAGMLTLLQSSLASLHGRTRRLHHSPC
                     EDQRFLDWAREYLPQHFRLPPSRMHRVVARHLEKAVAHRGMKLNVLGPRGSAKSTLVS
                     LAYPLWCLLEGKESYIWLVSDTRHQARAHLENIKAELLDNPQLCARYGPIRRKGTVWR
                     AEKVVLPSGAAIEAFGTGQRLRGRRQRADRPSLIIADDIQNDGHMTSSRLREHSRTWF
                     HGTLMAAGSPITNVVHLATALHYDALAMELSRTAGWQTLIFRAIERFPRRMDLWDRWQ
                     EIYCDADRPQAVHEARHFFLQHRREMEADAVVLWPEREDLYTLMCLRAEIGWAAFARE
                     KQNAPVSPELCEWPDEYFNEEIWYETTPRNLKLRVAVLDPSKGTDAKRGDYSALVFLG
                     VDAQGQMYVDADLARRPIAQMVTDAAEWCLRWRVQAFGVEANQFQELLAEPLEAEFRR
                     RGLMGFSPWLIRNHVNKLVRIRRLGPLLARRTLRFRQGSPGARLLVEQLRTFPIGDHD
                     DGPDALEMAIRLVGELARPAPDLSSTQRMVVW"
BASE COUNT          834 a         1338 c         1205 g          852 t
ORIGIN      
        1 aagcgaggcc ctttgggaaa cccggctgca aaccctcgaa acccgtttcg gtgaaagtga
       61 actgtgacgg aaaagaacag accacttcgc caggataagc cacagaaaca gctaaggtag
      121 gtcccatgca catccgtgat cgaatcaagg aattgcggcg tattcccgcc cgccaattgc
      181 gccccaatcc ccgaaactgg cgtgttcatc cggccaagca gcagcatgct ttgcgcggca
      241 tcctcgccga aatcggttac gccgatgccc tgttagcccg cgaactcccc gatggtaccc
      301 ttgaattgat cgacggccat ctccgcgccg agacaacgcc ggacgccatt gttcccgtgc
      361 ttgtcctcga tgtctccgag gccgaggcca acaagctact cgctcttctc gatcccctgg
      421 ccggtctggc cgaacaagac cacgacgccc ttgcccaact tgcccgtgac atcgaatttg
      481 aaagccctgt tctccaacag ctccttgccg aaacaacgca ggctgccgac aacccaccac
      541 aggagtcgcc accggagacg gaaacgagtg ccgtcgatcc cgtcccggag ttattccaag
      601 tcctagtgga atgccgcgat gaggacgacc agcggaggct gtttgaacgg ctttccgctg
      661 agggctacac ctgtcgcctc ctgacgctct aagccactgc cgatttgcta cgtccgcctt
      721 aaattgcgag tcacccatga aaaagcacgt cgtcgaagtg gttctcgaaa cgccgctcga
      781 tcggtccttc cgcgtcatgc aggtcgccgg gatgtttgat cttcccctgg aaaaagtgac
      841 ccgcgagcgg attgccgtcg agattcccca acaaaccgag gactggcaac tgggcttgat
      901 cgtcgggccg tcgggaagtg gcaaatcgac cctcgcccgc ctgctatttg gcgatcgcgt
      961 cagccgtact ttcacctggc ccgatgacca agccatcgtc gatgcctttg gccaatggcc
     1021 catcaaggag atcgtggggc ttttgacggc cgtgggcttc agttcgcccc cagcgtggtt
     1081 gcggccgtac cgggtcctca gcaacggaga acaggcccgc tgtgatttgg cccgcgcttt
     1141 tgccgaggcc cttcaatcgg gtccggtcga agatgcacag ccttggcacg tccttctccc
     1201 acccacagcc gatcgcagcc ggccgcttgt cgtctgtgac gaattcacga gcgtcgttga
     1261 tcgccacgtg gcccgagcca ttgccgcggg cttagccaaa cggcttcgaa aggggacact
     1321 tccctgccga tttgtggccg ttacctgtca ctacgatgtg gccgactggc tcaccccgga
     1381 ttgggtcatc gatatggcca cgcgggagtt ttctcggcga tcctttcggc gcccggaaat
     1441 caccttggca gtcgttcctt gccggcgtga agcgtggcgg gcctatcgcc gtcatcacta
     1501 tctaagcggc agtctcccct cggccgccga atcctatctg gccctatggg atggcgaggc
     1561 cgtggccttc tgtgccgtgg cttcccagat cggtcggcgg cactaccgcc gcatcagtcg
     1621 tttggttgtc ctcccggaat atcaagggat ggggatcggc tcggcatttc tcgaagcgat
     1681 ggcacgttac tatcgaaagc gggggcaacg tgtgggaatc acgacttcac atcccgccat
     1741 gattgcccat cttgctcgtt cgccccaatg gcgactgcgt tccctccgca aatggggctc
     1801 gcgcggtggt ggacgaaacc tgccgcaata ccgtggttcc ttcggtcgtg cggtcatgtc
     1861 ctttgagttc atcggccatg cctcgtaccg acagcccacc gccgcgtaat ggcgaagccg
     1921 ttccagagga tcaggaaccg cggaaacgac ctaagactct tggcggaagg aaacagttgt
     1981 gacggaaacc cagggacaag agacagcgcg tcgcggtttg gacgaattcc gcaaacggga
     2041 aattttggca atcctcagtg tgggttgtag tcgtcgcaca gcagcccgtt atgtgggatg
     2101 ttcgccctcc acgattcgcc gcaccgccga acgtgacccc gccttcgctg aggccctcca
     2161 caaggccgaa agcaaggccc aaatcctctt tatgcaaaac atcgcggcag ctgcccgcaa
     2221 agaacagtac tggcgtgccg cggcctgggc gctggaacgc ctcaacccgg aagactacgc
     2281 cgctcgttcc ccccatgccg tgacattgga gcaaattcga accctggtct cagagtttgc
     2341 ccgcatcatc gtggaagaag tccccatcgc cgcccaccgg aaaaagattc tccgccgctt
     2401 ggaacgcctg ggtcgggaat gggatgtccc tccctctgtg gccgtcaatc gcactgaccc
     2461 ttcggacgaa tgaaaggacg cccaagatgc cgcggaacac atttgccgag agtgacctgg
     2521 ttcttaacca gccgcaggcc gcggggatgc tcaccttgtt acaatcctcc ctggcaagct
     2581 tgcacggccg gacccggcgg ctgcaccatt cgccctgtga agatcaacga ttcctcgatt
     2641 gggcccgaga atacctgccg caacacttcc ggttgccacc ttcgcgcatg catcgggtcg
     2701 ttgcccgcca tttggaaaag gccgtcgcgc accgcgggat gaagctcaat gtcctagggc
     2761 cgcgggggtc tgccaaatcg acattggttt ccttggccta tcccctatgg tgtttgttgg
     2821 aaggcaaaga aagctacatc tggttggtct ccgatactcg gcatcaagcg cgtgcccatt
     2881 tggaaaacat caaggccgaa ctgctggaca accctcagct ttgcgcacga tacggaccga
     2941 tcaggcggaa aggcacggtc tggcgggcag aaaaggttgt ccttccgtca ggggccgcca
     3001 ttgaggcctt cggcaccggc caacgcttgc ggggacgacg acaacgcgcc gatcgcccca
     3061 gcctgatcat cgccgatgac attcaaaacg acggacacat gacgtctagc cgcttgcggg
     3121 aacattcacg gacctggttc cacggcacgt tgatggccgc gggcagcccg atcaccaacg
     3181 tcgttcacct cgctacggcc ttgcactacg acgccctggc catggagtta tcccgaaccg
     3241 ccggttggca gacgctgatc tttcgcgcca ttgaacgctt tccgcggcga atggaccttt
     3301 gggatcgctg gcaagaaatc tactgcgacg ccgatcggcc ccaggcggtc catgaggccc
     3361 gtcatttctt cctccagcac cgtcgggaaa tggaagccga tgcggtcgtc ctttggccgg
     3421 agcgagaaga cctttacacc ctgatgtgct tgcgggctga gatcggatgg gcggccttcg
     3481 ccagagaaaa acaaaacgct ccggtctcgc cagaattgtg cgaatggccc gacgaatact
     3541 tcaacgaaga aatttggtac gaaacgactc cgcgcaatct aaaactgcga gttgccgtcc
     3601 ttgatcccag taaggggacc gacgccaagc gtggtgatta ctcggcgttg gtctttctgg
     3661 gagttgatgc tcagggccag atgtacgtgg atgcggacct ggcccgtcgc ccaattgccc
     3721 aaatggtcac cgatgccgcc gagtggtgtc tccgctggcg ggtccaggcc tttggcgtgg
     3781 aggccaacca gtttcaagaa ctccttgccg agccgctgga agcggagttt cgtcgccgtg
     3841 ggctcatggg gttttcaccc tggttaatcc gcaaccatgt caacaaactg gtgcggattc
     3901 gtcgtttggg gccgcttctc gcgcggcgaa cacttcgctt ccggcaaggc tcgcccggtg
     3961 ctcgactcct ggtcgaacag ctccgcacct ttcccattgg tgaccacgac gatggccccg
     4021 acgccttgga aatggcaatt cgcctcgttg gcgaactcgc gcgaccggcc cccgacctct
     4081 cctccacgca gcggatggtt gtctggtgat cgtagcgatt gccttggttg tttcctgttc
     4141 gagccatcac gacggtcagt aaaagaagaa aggaagaacc atgctgccgt atgtccggca
     4201 cgagggtaag gcccccgcct gtgtcgttc
//
LOCUS       DAOPTR010000218         4201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_17031_length_4201_cov_3.538109, whole genome shotgun sequence.
ACCESSION   DAOPTR010000218 DAOPTR010000000
VERSION     DAOPTR010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17031_length_4201_cov_3.538109"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            252..1592
                     /locus_tag="PKI05_05460"
     CDS             252..1592
                     /locus_tag="PKI05_05460"
                     /inference="COORDINATES: protein motif:HMM:NF013715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HOA51683.1"
                     /translation="MMSQPLEKPVSSSPSGKRRWYQAIGPALITACVVFGPGSLIISS
                     NVGAVYRFQFLWLLILTGLLMGTYVAMAARIGVCGGASPCTLLARRLGRPVAFFVGMV
                     LCLVCATFQFSNNLAVALAAGAFLPERINLTIGGTVQQLPVLPTLLIILNGMIILFLF
                     VGRQIYKTLERGMKVMVAIILLCFVFNLVVARPDVIGVLRGFIPSWPEGLQLGIPKKI
                     GQTIDDPMILLASLLGTTFSVGGAFFQGNLVREKNWSIHDYQHGIIDSIAGVAVLTGV
                     SMIIMITAATVIPGKPAHDVGELARSLEPLLGPTSYTLFCLGLFAVAMNPIVINAVIG
                     GTIFADGLGLPARLNDRAPRILTVLVMLIGMGVALWALTSGQRPVPLIIFGQALTVIG
                     NPLMAAALLWLANQPDIMGEKRNRLVSNLLGGLGFLVVLLVAARVLWRVILHFA"
     gene            complement(1701..3245)
                     /locus_tag="PKI05_05465"
     CDS             complement(1701..3245)
                     /locus_tag="PKI05_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51684.1"
                     /translation="MNWLRTAYRLFSFFDALYLAGNFLYRRSLRYTFTTAFVSFVGYL
                     GNLVPGVKTYDARVAILMPLLVGSFMLTTGLLLKLIPLAFRSRLLNVAQAADLDLMEN
                     YRKWNQEMHLESLWERVYQYEWKLGTHLTGVRPHEAECPSELCSDEGLPSDVVERGRI
                     KFLRRARFGLARPQPESRQRHHLGIDLRFMEDWYNGGYFDPNDVKLDEQQAAAVPIMI
                     VRSLVHYGFWDSVRDLPLRLFGRIWFRLITRSIALRVGEAVIRLNRQFNTDYFNAQAL
                     LWPEESEEPWVAALGEDARQALLRERARILRCVFNNPTEGVLMLDRFLVPLFLFATDL
                     RARFDPEYLDGSLGYDLWSDLKWVGFPAFKRMHFARLIQRAARESRLIKEVLASQEMG
                     DLTPHPLGEGNQEILRAIRIATFINWEQLGTLMRRFGKKRKYTPELRRVIREKLQKVI
                     ENRHRFSAYLVSIRVHHELTRLHRATYHRLLLDLFRTCEEIPRDLNAQCSRLITQGQS
                     LLDLDS"
     gene            complement(3381..>4201)
                     /locus_tag="PKI05_05470"
     CDS             complement(3381..>4201)
                     /locus_tag="PKI05_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA51685.1"
                     /translation="LNTHWLGPLPILAACEYGKAVYLGSRVVLSLEETTQIRDAVRQS
                     GVAFMVEFARRYSPATVRLKELMATVLGSPRLLFCHRRLSHYLKCNGLSHITPSQALH
                     LDLIELVDWCRFVAGRSPIKVMGVVHRSSHPDDVDYMMMSLNFGSASEGREPNVLAQI
                     SCGKYLPPHWREAVNYRPLAELQVVCERGVAFVDLPTTLVWFDEAGRHQESFDNERPL
                     GERLLFCFHRMVTSLVQRMSDIEDLHQTQRIIESLEQSARTGQCVFLSSERHKG"
BASE COUNT          990 a         1130 c         1097 g          984 t
ORIGIN      
        1 gccagaccct cagatcgtga ggaaccccgc cgtgaatcga gctcccgaaa tcgtgccgat
       61 atccccgaag ctgcgcctga tttagttata ctgacatatt ctcgtaatgc aaatgattcg
      121 tgctgctctg gggctagtga aatgtggcga ttgactccca ccaggatttc gtttatccat
      181 ggatgccaac agaagccggt tgcttgttcg aaacaatcgc agtgagaggc catttgtgtt
      241 gcagggagcc gatgatgtcc cagccgctgg aaaagccggt gtcgtccagt ccatccggaa
      301 aacggcgttg gtatcaggcc attggtccgg cactgatcac cgcttgcgtg gtatttgggc
      361 cgggcagttt gatcattagc tccaacgtgg gggcagtcta tcgctttcag tttctctggt
      421 tgctcatcct gaccgggcta ttaatgggga cgtatgttgc catggccgca cggatcggtg
      481 tctgcggcgg ggccagcccc tgcacactct tggcccgtcg cttggggcgt cctgtcgcgt
      541 tttttgtggg aatggttctt tgcctggtct gcgccacatt tcagttttcc aataatcttg
      601 ccgtggcctt ggccgctggc gcctttcttc ctgaaaggat caatctcacg attgggggaa
      661 cggtccagca attgccggta cttcctacgc ttttgatcat tctcaatggt atgatcatcc
      721 tctttctgtt tgtcggtcgc caaatctaca aaaccttgga acgagggatg aaggtcatgg
      781 tggccatcat tcttttgtgt tttgttttca atcttgtggt cgcccgcccc gatgtgattg
      841 gcgttttgcg tgggtttatc cccagttggc cggagggcct tcagcttggc attccgaaaa
      901 agattggcca aacaattgac gatccgatga ttctcttagc ttcactctta ggaacgacgt
      961 tttccgtggg aggtgcgttt ttccagggaa atctcgtccg cgaaaagaac tggtcgattc
     1021 acgattacca gcacggaatc attgactcga ttgcaggggt agcagtactg acgggcgtca
     1081 gcatgatcat tatgatcacc gccgccacag tcatcccggg aaaaccggct cacgatgtgg
     1141 gtgagctggc cagatcgctc gagcccctat tgggacccac ctcttacact ctgttttgcc
     1201 tggggctgtt cgccgtggct atgaacccga tcgtgatcaa cgccgtcatc gggggaacca
     1261 tttttgccga tggtttagga cttcctgccc gactgaatga ccgcgccccc cgcatcctaa
     1321 ccgtccttgt tatgctcatc gggatgggcg tcgctttgtg ggcgttgaca agtggtcagc
     1381 gcccggtgcc cttaatcatc ttcggtcagg ccctcaccgt catagggaat cccttaatgg
     1441 cggccgcttt actctggctg gctaatcagc ccgatatcat gggggaaaag cgcaaccgtc
     1501 tggtatccaa tctccttggt ggattgggat ttctcgttgt gttattggtc gcggcacgtg
     1561 ttctctggcg ggtgatctta cacttcgcgt aacgggcaac ttcatacgac caaacgtgtg
     1621 gatcggctgc gtaaaacgta cgttaccgag gatggtcagg agcaacgcaa acgtgaaact
     1681 gcacgaccgc aacattttct ttaggaatcg agatcgagaa gcgattggcc ctgagtgata
     1741 agtctactgc actgagcatt taaatctcga ggaatctctt cacacgttcg gaacagatcg
     1801 aggagcagcc gatggtacgt cgcccgatgt agtcgcgtta gttcatgatg aacgcggatg
     1861 gacaccaaat acgcactaaa acggtgacgg ttttcaatga ctttctgcaa cttttctcgt
     1921 atcacccggc ggagctccgg agtgtattta cgcttcttgc caaaccgtcg catcaaggtc
     1981 cccaactgct cccagttaat gaatgtggca atccttatcg cccgaaggat ctcttgattg
     2041 ccttccccga gcggatgagg agtaaggtca cccatctcct gagacgctaa aacttctttg
     2101 atcaggcgac tctcgcgggc ggcacgctgg attagcctag caaaatgcat ccgtttgaat
     2161 gcgggaaatc caacccattt gaggtccgac caaaggtcat aacctaaact tccatcgagg
     2221 tactcaggat caaatcgcgc gcggaggtct gtcgcaaata ggaacaacgg caccaggaaa
     2281 cgatcaagca tcaagacgcc ttccgtggga ttgttgaaca cacagcgcaa aattctagcc
     2341 cgttcgcgaa gcagggcctg acgagcatcc tcaccaaggg ccgctaccca gggttcttcg
     2401 gattcttccg gccacaaaag ggcttgagcg ttgaaatagt cggtgttgaa ctgccgattg
     2461 agcctaatca cggcttcgcc cactcgcaag gcaatggacc gcgtgatcaa gcgaaaccaa
     2521 atccttccga aaaggcgtaa gggtaaatca cgcacggagt cccaaaaccc gtaatgaacg
     2581 agggaccgca caatcataat tgggaccgcc gcggcttgtt gctcgtcgag cttcacgtca
     2641 ttcggatcaa agtacccgcc attgtaccaa tcctccatga agcggaggtc aatgcccagg
     2701 tggtggcgct gtcgtgattc cggctgaggg cgtgctaacc caaatcgggc ccgtcgcaaa
     2761 aacttgattc ttcctctctc cacaacgtcg gacggaaggc cctcgtcgga acacagctca
     2821 gagggacact ctgcctcatg aggtcgcacc ccagtgagat gtgtcccgag tttccattca
     2881 tactggtaaa cccgttccca aagcgattcc aggtgcattt cctggttcca cttgcgataa
     2941 ttctccataa ggtccaaatc ggcagcctgc gcaacattca aaagccgcga tctgaacgcg
     3001 agaggaatta atttgaggag aagcccggtc gtcagcataa aactgcctac taaaagaggc
     3061 atcaaaattg cgacacgtgc gtcgtaggtt ttcacgccgg ggacgagatt acctaggtat
     3121 ccaacaaaag agacgaacgc ggtggtaaac gtgtatcgaa ggcttcgtcg gtaaaggaag
     3181 ttccctgcaa ggtaaagggc atcgaaaaaa ctgaagagcc gatacgccgt acgcaaccag
     3241 ttcatcacgc ccctcaattg ctgtagttaa ggtggcccga caattcagtg ttggtgggaa
     3301 ctttgttcga ttgcgcccct tgattttaat caaaaaaggt taggtctgcg aacctgagcc
     3361 ttcgttgaag cgagcgtttg tcaaccctta tgtcgctcgc tgcttagaaa aacgcattgt
     3421 ccggtcctcg cgctctgttc aagtgactca atgattcttt gcgtttgatg aaggtcttca
     3481 atatcactca tgcgctgcac gagactggtg accatccgat gaaaacaaaa tagcagtcgt
     3541 tccccaagcg gacgttcatt atcgaatgac tcctggtgac gtccggcctc gtcaaaccaa
     3601 acgagagtgg ttgggagatc cacaaaggca accccccgtt cgcaaaccac ctgaagctcg
     3661 gccaagggac gataattgac ggcctcacgc caatggggcg gcaaatactt accacagcta
     3721 atttgggcaa gaacatttgg ctcgcgcccc tctgaggccg aaccgaaatt cagactcatc
     3781 atcatgtaat caacgtcgtc aggatgcgac gaacgatgaa ccacgcccat caccttgata
     3841 ggagaccgtc cggcaacaaa ccggcaccaa tccacaagtt cgatgaggtc caagtgcaat
     3901 gcttgacttg gcgtgatatg ggacaagcca ttgcacttaa gatagtgtga cagtcggcga
     3961 tgacagaaca acagccgcgg tgaacccaag acagtggcca tcaattcttt aagacggaca
     4021 gtggcggggg aataccgtcg ggcaaactcc accataaaag ccacaccgga ttggcggacc
     4081 gcatctcgaa tctgggtcgt ttcctccagc gacaaaacga cccgcgaacc taggtacacc
     4141 gcctttccat attcgcaggc cgccaaaatg ggcaagggac ctagccaatg ggtgttcaaa
     4201 a
//
LOCUS       DAOPTR010000219         4198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_17045_length_4198_cov_3.664977, whole genome shotgun sequence.
ACCESSION   DAOPTR010000219 DAOPTR010000000
VERSION     DAOPTR010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17045_length_4198_cov_3.664977"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="PKI05_05475"
     CDS             complement(<1..1116)
                     /locus_tag="PKI05_05475"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOA51686.1"
                     /translation="MAERVGVYVCHCGSNIAGMVDVEEVARWAGANLKDVVVARDYKF
                     MCSSLGQAMIEEDIKKEGLTRVVVAACSPHLHEKTFRRACENAGLNPYLCQMTNIREH
                     VSWVSKDRAKATEKARALVAAAVERVKHQAPLEPFFVKVNPATLVIGGGIAGLQATLE
                     LADAGYPVYLVERTPSIGGHMAQFDKTFPTLDCSACILTPKMSEAGQHPNVTLFTYAE
                     VEEVSGSVGHFRVKIRKKARYVEEDKCTGCGICVEKCPRKVVDHDFEAGLGYRKAIYT
                     PFPQAVPRIPVIDVESCIWFERQKCRACEKLCPTGAINFDQKDEEITLDVGNIIVATG
                     WKMFDCKKIPQYGYGRLANVFTALEFERMCNAAGPTGG"
     gene            complement(1231..2121)
                     /locus_tag="PKI05_05480"
     CDS             complement(1231..2121)
                     /locus_tag="PKI05_05480"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HOA51687.1"
                     /translation="MKYAYYPGCSLECSSAAYDMSVRAVADLLGIELREIDDWNCCGA
                     TEYFSQDELTACAVIARNLALIEPQLDQVVAPCAACYLNLRKVDSLMKEDPALADKIN
                     QCLAAGRLHYDPGRVRVRHLLDLIHTDIGETAIKDKVVQPLTGLRVACYYGCQIVRPY
                     DGFDNPEQPVKLDELMTWVGAEVVDFPVKAHCCGGHMTQISEPQAFELIRRLLHNAAE
                     YRADLILCMCPMCQLNLDAYQNRVNSFFGTNYQIPIIFFTQLLGVAFGLDWKKLGFGK
                     EIVPAEPVLRKKLTAPAATA"
     gene            complement(2118..2693)
                     /locus_tag="PKI05_05485"
     CDS             complement(2118..2693)
                     /locus_tag="PKI05_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOA51688.1"
                     /translation="MVGTTLELRGSPFLHEIIQEVPGGERIVRCLQCGTCGGSCPNGA
                     DMQYTPRTIFAMIAANQREKVLKANTMWFCVSCYFCVSRCPQKIPITDIMYGLKRIAI
                     RERMYQDTDAPALAKTFTDLLDKYGRSFELGLASRYYLFNRPIAMLKMGPMGLSMFTR
                     GRMSLVPTKIKNIEQLQSIIRKARELGGTQQ"
     gene            3206..>4198
                     /locus_tag="PKI05_05490"
     CDS             3206..>4198
                     /locus_tag="PKI05_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51689.1"
                     /translation="MKTRAFWLIAVLGLVVTFGLWRLAISYRTAERREDGERITGQLA
                     TSLSQRFEKFTNVVDVLRTLRAASREIDDTEWRTALDVLGMSNFPEWETAGITFLSSA
                     PDEKAEASDHVGLPLTFQHLTVPINDPLARKQIEALLSQLVRLLPQAYCDPVVGFVIP
                     LENRFVFLRPVKNLQPICERNAQMSAEIARVFRETPFVLGIFNSQRGLAQTAEKHQPK
                     GTVRLLWTAGNVNSPTELARWSNSRPGSHSASFGCYRSALSWDGPTTILEYQPDTLRA
                     EETTELLPALAIGFGLLFTSLGCALVAVSTRFRRGVRLLRRRYKTLLDEQEKRYY"
BASE COUNT          916 a         1277 c         1078 g          927 t
ORIGIN      
        1 cccgcccgtc ggcccggccg cattacacat ccgctcgaat tccaaggccg tgaagacgtt
       61 cgccagacgt ccgtaaccat actgcggaat cttcttgcag tcgaacatct tccagccggt
      121 tgccacgata atattaccca catcgagcgt gatctcttcg tctttttggt cgaagttgat
      181 ggcgcccgtg ggacagagct tttcgcaggc ccggcatttc tgcctttcaa accaaataca
      241 cgattccacg tcaatgacgg ggattctcgg aacggcttgg gggaacggcg tgtagatcgc
      301 cttgcggtat cctaagccgg cctcaaaatc gtgatccacc acctttcgtg ggcatttttc
      361 cacgcaaatc ccgcagcccg tgcatttgtc ctcttccaca tagcgggctt tcttgcggat
      421 tttgacgcgg aaatggccca ccgagccact cacctcctcc acctcagcat aggtgaacaa
      481 cgtcacgttg ggatgctgtc cggcctcgga catcttcggc gtcaagatac atgccgagca
      541 gtccaaagtg ggaaaggtct tgtcgaactg cgccatgtgg ccgccaatgc tgggtgttcg
      601 ttcgacgaga taaactggat agcccgcgtc ggccaattcc aatgtggcct gcaaaccggc
      661 gatgccgcca ccgatcacga gcgtggccgg gttaaccttc acaaagaatg gttccagcgg
      721 tgcttggtgt ttgacgcgtt ccacggcggc ggcgaccaag gcccgggcct tttccgtggc
      781 tttggcccga tctttggaaa cccaggagac gtgctcgcgg atgttcgtca tttggcacag
      841 atacggattg agccccgcat tctcgcatgc ccgacggaac gtcttctcgt gcaaatgcgg
      901 ggaacaagcc gccacaacca cgcgcgtcag gccttccttc ttgatgtcct cctcaatcat
      961 ggcctgacca agcgacgagc acatgaactt gtaatcgcgg gcaacgacga cgtctttaag
     1021 atttgccccg gcccaacggg ccacctcttc cacatcaacc atccccgcga tattacttcc
     1081 acagtggcaa acataaacgc ccacgcgttc ggccatggga tctctctcct caaataatct
     1141 gtctggtccc aactcgatgg atcacctcaa gtcaaatagc caaaggcagc ggctgaccga
     1201 ccacggcccc gctgggccgc ccccgacacc tcacgccgtc gccgccgggg ccgtcagttt
     1261 cttccgtagc actggctccg ccggaacaat ctctttgcca aaaccgagct tcttccaatc
     1321 caaaccaaaa gccactccca ataactgcgt gaagaaaatg atgggaattt ggtaatttgt
     1381 gccaaagaaa ctgttgacgc ggttttggta agcgtccagg ttaagctggc acatcgggca
     1441 catgcacagg atcaagtccg cccgatactc cgccgcattg tggagcagtc ggcgaatcag
     1501 ttcaaaggcc tgcggctcgc tgatttgtgt catatgaccg ccacagcagt gagccttgac
     1561 cggaaaatcg acgacttccg ctcctaccca ggtcatcaac tcgtccaatt tgacgggctg
     1621 ctcggggttg tcgaacccgt cataggggcg aacaatctgg catccgtagt aacaggccac
     1681 ccgcaaaccc gtgagaggct gaacgacttt atctttgatg gccgtctcgc caatatccgt
     1741 gtggatcaaa tccaaaagat ggcggacgcg gacccgaccg ggatcatagt gcagacgtcc
     1801 cgccgccaag cactgattga tcttgtcggc cagggcgggg tcttccttca tcaaggaatc
     1861 gaccttcctc aagttcaagt agcaggccgc acacggggcc accacctggt cgagctgcgg
     1921 ctcaataagg gcgagatttc tagctatgac ggcgcatgcc gtcaactcgt cctggctaaa
     1981 gtactctgtt gcgccgcaac aattccagtc gtcgatttcc cgcagctcaa tccccaaaag
     2041 atcggccacg gcacgcaccg acatgtcgta cgcagcactg gaacactcta acgaacaacc
     2101 ggggtaatag gcatatttca ctgttgggta cctcccagct cacgagcttt acgaatgatg
     2161 gactggagct gctcgatatt cttaatcttg gtcggcacaa gggacatgcg tccccgggta
     2221 aacatggaga gccccatcgg ccccattttg agcatcgcaa ttggtcgatt gaacagatag
     2281 tatcggcttg ccaaccctag ctcgaaactc cgaccgtatt tgtccagcag gtcggtgaac
     2341 gtcttggcca gcgcgggggc atctgtgtcc tggtacatgc gttcgcggat agcgatccgc
     2401 ttcagcccat acatgatgtc cgtgatcggg atcttctgcg gacaccgact gacacagaag
     2461 tagcaggaga cacagaacca catcgtgttc gccttgagga ccttctcccg ctggttggca
     2521 gcaatcatgg caaagatggt gcggggcgtg tattgcatgt ctgcaccgtt gggacaactc
     2581 cccccgcagg tgccacattg caggcagcgg acgatccgct caccccccgg tacttcctga
     2641 atgatctcat gaagaaaggg cgaaccccgc agttccaatg ttgttccaac cattgagtca
     2701 ccccaaataa gacatattta tctgttgcaa acttataaac acgctccgta cgtgcctagc
     2761 tacgcaaatc gaataccgat taccgcttgc cgctgcccaa ccttaccgat cttacgtatt
     2821 aaactatact tatggcgaat ccgccatctc aggaaggctg gctgcccttt cgcacaaaaa
     2881 acgaaaatcc gcggtctccc gcgattttcc gcgacataaa aacgacttct cattccattc
     2941 ggcaccgcgc cccgttgaac tgtggcccta tcccgcaatt atgcgaaata tcacggttgt
     3001 tcgggacttc ccccccgttg gcaagggcca gaaagcagct tcaggatggg ttgtctcctc
     3061 tcatgggacc tatattttcc taaaggccgc ggggatattc ttttttcttc gtcctggcgg
     3121 aaaacccctc ccgcaggctt atcatgggtc ctttggagag cgcacgtaat ttatttcgat
     3181 gaaaacgctg ctttggttca tttctttgaa gacacgagcg ttttggctga ttgcggtcct
     3241 gggtttggtc gtaaccttcg gcctctggag gctcgcgatc tcctaccgca ccgccgagcg
     3301 gcgcgaagat ggcgagcgga tcacgggaca actcgccacg tcgctttcgc aacggtttga
     3361 aaagttcacg aacgtcgtgg atgtcctccg taccttgcgg gctgccagtc gcgaaattga
     3421 cgacaccgaa tggcggaccg ccttggacgt tctcggcatg tcgaacttcc ccgagtggga
     3481 aaccgccgga atcactttcc tatcttcagc ccctgacgaa aaggccgagg cgtcggatca
     3541 cgtcggcctg ccgctgacat tccagcacct gacggttcca atcaacgacc cgcttgccag
     3601 gaagcagatt gaagccctcc tttcgcaact ggtacggctc ctcccgcaag cttactgcga
     3661 tcctgtggta ggttttgtga ttcccctcga aaaccgcttc gtcttcttga gaccggttaa
     3721 gaacctccaa ccgatctgcg agcggaacgc ccagatgtct gccgaaatcg cccgcgtgtt
     3781 ccgcgagacg cctttcgtgc tgggcatctt taacagccag aggggtctcg cgcaaacggc
     3841 ggagaagcac cagccaaagg ggacggtccg gctgttgtgg acagcaggca acgtaaactc
     3901 accgacagaa ttggcacgat ggtcaaactc tcgacccggg tcgcattcag catccttcgg
     3961 ctgctatcgg agtgctcttt cctgggacgg gcccactacc atcctggaat accagcctga
     4021 cacactgcga gcggaagaaa caaccgagct tttacccgcc cttgccatcg gttttggtct
     4081 gctttttacg tccctgggct gcgctctggt tgccgtgtcc acaagatttc ggcggggcgt
     4141 gcgccttctc cggcggagat ataaaaccct cttagatgaa caagaaaagc ggtactac
//
LOCUS       DAOPTR010000220         4194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_17069_length_4194_cov_3.405412, whole genome shotgun sequence.
ACCESSION   DAOPTR010000220 DAOPTR010000000
VERSION     DAOPTR010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17069_length_4194_cov_3.405412"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..1084
                     /locus_tag="PKI05_05495"
     CDS             248..1084
                     /locus_tag="PKI05_05495"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51690.1"
                     /translation="MTRCLLLINEPGSGPWNMAMDEFCATHAEREDVPILRLYRWREP
                     TLSLGYFQRYQDYQRHRLNGLCPVVRRPSGGGAILHDLEWTYSLAFPTSRVTDRDRLS
                     FYRAVHQTILTWLDSLGLKGEIIQEQPPPCKSNNTDCAFLCFQRRAVGDVVVPLSPEM
                     HTLVSAGATGEPVPTDVKIVGSAQRRYKNTVLQHGSILLGRSVLTPELPGVMEILASK
                     GIQLTHDEISYHFVSHWPQTVARALGWRLQPWTLSLTDSPEFAELEAKYLSRAWTCKS
                     QA"
     gene            complement(1125..3701)
                     /locus_tag="PKI05_05500"
     CDS             complement(1125..3701)
                     /locus_tag="PKI05_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51691.1"
                     /translation="MRLVVHALAFLSLLGCARDVLAEASGIFPKAVTRKQSLTLGFGE
                     GTQGFEPVSQCRIAVENGRLVVESTGHDPYIVKAMNLPGEAYEVSVRLRTTVRSGGAI
                     YWTSQKSLTWGEDKSRHFFLIADGEWHSYTVRIDTPGGLRGLRIDPGTAPGIYEIESI
                     QLTELQTCPINIEFIERTDEGVQFHIQNASNAPVTVRMEQHSLVIPAGQAIDIIRKFD
                     GQRPVELVTLDLQLPDFTTDLRHSVVVYSDSAPAEWMAIATGGDTAVLVELAAKDNVL
                     RIRRGETVLAALAPIVAPADPNGAGLIATIPLLQATPIPNGVQIQGKGVQGTIAVDKD
                     ELILNLEATDENSEIEGPVVRAFGLLEQGVFAGLEYLGKGEKSSSTLDIETEEHIRYA
                     PDRLNVTMPLMAFVTDRASVGLTWNDMGLQPVYATPNVFDGTQEHRMSLRGHHIEARL
                     RIAQGMVEDMILWATKRWGLPPLPEPPRSESEQFQLCLWALTDGPIRNEQGWGHCVEP
                     NWARRFHVDMVSTIWRLSGTLPDVPHLVPGGGHLSDDSAWFLTGRAAEWLAHMRNQAR
                     GLMAQQQPDGSFRYSGKYLKGHYEDTASGYCARPAAVLLEYAKLTGDKQALEAGLKTL
                     DYMKRFRVPRGAQTWELSLHTPDILASAHLVSAYVRGYELTKRQDYLDEARRWALSGI
                     PFVYLWAEHPIMLYATIPVYGATNWRAPNWIGLPVQWCGLVYAYALTELAPYDNTLDW
                     RHLAEGILIAGEQMQVPREEGDYAGLLPDSFHLRYQRRQGPFINPCALALLRSAIKGR
                     PHRLAVAIGDNHRVVAPFPVKIEGNEAVIQARAGVSYQIVIDGEQIKTIASQGEDRIS
                     LE"
BASE COUNT          964 a         1149 c         1116 g          965 t
ORIGIN      
        1 agtgtaacta attccatcag gccgtaagat agccgcacca gggtagcatt ccggttgctt
       61 cttgcgggag tctcattgac gagggcatat tgttgaatca atcgcttaca gtcgatccga
      121 attcaatgcg gagcaagacg aaactattgg gtgcgtggtt tctttttgta cctgcctcgc
      181 gaagatgtcc cttcttgcgg gttagctttt ggtggtgaga acgcaacttt catgatacgg
      241 aaaaggcatg acacgttgtt tgctcttaat caatgagccg ggtagtgggc catggaacat
      301 ggcgatggac gagttctgcg cgacccatgc ggaaagagaa gacgtcccca ttttgcggct
      361 ctaccggtgg agagaaccaa ccctctcgtt gggatatttt caacgctatc aagactatca
      421 acggcatcgc ctaaacgggc tttgccctgt cgttcgccgt cccagcggtg gaggggccat
      481 cctccacgat ttggaatgga cctacagttt ggcttttccc accagccgtg tgacagaccg
      541 cgatcggctt tcgttctatc gtgcggttca ccagacgatt ttgacctggc tcgactcgct
      601 gggactaaag ggcgaaatca ttcaggagca accacccccg tgtaagtcga ataacaccga
      661 ttgtgcgttc ctgtgttttc agcgtcgggc agttggtgat gttgtggttc ccctttcccc
      721 ggagatgcac acactagtat cggctggggc cacaggggaa ccagtgccca cggacgtcaa
      781 aatagtgggt agtgcccagc ggcgctacaa gaacaccgtc cttcagcacg gcagcatctt
      841 attgggccga tcagtgctta ccccggaatt gcccggcgtg atggaaattc tggccagcaa
      901 ggggatccaa ctgacccatg acgaaataag ttaccatttt gtttcacact ggccccaaac
      961 agtggctcgt gcgctgggat ggcgattgca accttggacg ctttcattga ccgattcgcc
     1021 cgaatttgct gaactcgaag caaaatacct atcccgcgct tggacgtgca aatcacaagc
     1081 gtaagcacat cgggaatcaa cggggaaatc tcttgaccga ccgctcactc caaggaaatt
     1141 cggtcttctc cctgggaagc gatcgtcttg atctgttcgc catcaatgac aatttgatac
     1201 gacacgcctg cccgagcctg aatgacggcc tcatttccct cgatcttgac gggaaaaggc
     1261 gcaacgaccc gatggttgtc accaatagcg acggccaatc ggtgcggtcg ccccttgatg
     1321 gccgaacgaa gaagggcaag ggcacagggg ttgataaaag gcccctgtct tcgctgatag
     1381 cgaaggtgga atgaatccgg caatagaccg gcatagtctc cctcctccct ggggacttgc
     1441 atttgttcgc cagcgatcaa gatgccctcg gcaagatgac gccaatccag ggtattgtcg
     1501 taaggggcga gctccgtaag cgcgtaagca tagaccagac cgcaccattg tacggggaga
     1561 ccgatccaat tcggcgcccg ccaatttgta gccccataaa cgggaatcgt ggcataaagc
     1621 atgataggat gttctgccca aagataaaca aacggaattc cactcaaggc ccagcgtcgg
     1681 gcttcatcaa ggtaatcttg acgcttcgtc agctcatatc cccgaacgta ggctgaaaca
     1741 aggtgcgccg aagccaaaat gtcgggcgta tgcaaagaga gttcccacgt ctgtgcccca
     1801 cgagggacac gaaaacgctt catatagtcc agcgtcttga gaccggcctc aagggcctgc
     1861 ttgtctccgg taagtttggc atactccagc aacacggcgg cgggacgtgc acagtagccg
     1921 ctcgcggtgt cctcataatg tccttttaag tattttccag aataccgaaa ggaaccatca
     1981 ggctgttgtt gcgccatgag ccccctggcc tggttccgca tgtgcgcgag ccactcggcg
     2041 gcacggcccg tcaagaacca tgcactgtcg tccgagaggt gaccaccgcc gggaacaaga
     2101 tggggtacat caggcaatgt ccccgatagc cgccatatcg tagaaaccat atcgacatga
     2161 aagcgacgag cccagttcgg ttctacgcag tgtccccaac cttgctcgtt gcgaatggga
     2221 ccatcggtca acgcccacag acacaattga aattgctccg attcactccg tggtggttca
     2281 ggcaaaggcg gtaatcccca ccgcttggtt gcccaaagaa tcatgtcctc aaccatccct
     2341 tgcgcgatgc gcagacgggc ctcaatgtga tgccctcgaa gcgacatgcg atgctcttgg
     2401 gttccatcaa agacattggg ggtggcgtaa acgggctgca atcccatgtc attccatgtg
     2461 agtcccaccg acgcccgatc ggtaacaaaa gccattaagg gcatggtcac atttagccga
     2521 tcaggggcat atcggatgtg ctcttctgtt tcaatatcca acgtggaaga actcttttct
     2581 ccctttccga gatactccag gccggcaaag acgccttgct ccaagagacc aaatgctcgc
     2641 accaccgggc cttcgatctc cgagttttca tctgtggcct ccagatttag gatgagttcg
     2701 tccttgtcca cagcaatcgt tccttgaacc cctttccctt gaatttggac accattggga
     2761 ataggagtgg cctggagcag gggaattgtt gcaatcaggc cagcaccatt aggatcagcc
     2821 ggggccacaa tgggtgccag ggctgctagt accgtctccc cgcgacggat tcgcaaaaca
     2881 ttgtctttag ccgcgagctc cacaagcacg gcggtgtccc cacctgtggc gatggccatc
     2941 cattcggccg gggcagaatc ggaatacacc acgactgaat gccgcaggtc ggtggtgaaa
     3001 tcggggagct gcaaatccag ggttaccaat tccactggcc gttgtccgtc aaacttacgg
     3061 atgatgtcga tggcctgacc ggccgggata acaagcgagt gctgctccat ccgtacagtg
     3121 acaggagcat tgcttgcatt ctgtatatgg aactgcacac cctcgtctgt cctttcgatg
     3181 aattcgatat tgatgggaca tgtttgtaac tctgtcaact gaatgctttc gatctcatag
     3241 atccccggtg ccgtgcccgg atcaattcgc aatccgcgga ggccaccggg tgtgtcgatt
     3301 cgaaccgtgt agctgtgcca ctctccatcg gcaatgagga aaaaatgccg ggatttatcc
     3361 tctccccaag ttagtgactt ttgactagtc cagtagatgg ccccaccgct acgcaccgtg
     3421 gttcgtaaac gaacggagac ctcataggcc tcgcccggaa gattcatcgc cttaacgatg
     3481 tacgggtcat gcccggtgct ctcaaccacc agtcgcccat tttccaccgc aattcgacat
     3541 tggctgacgg gctcaaagcc ttgcgttcct tctccaaagc ccagcgttag ggactgcttt
     3601 cgtgttacgg ccttcggaaa gatccccgac gcttccgcca atacatcgcg ggcgcatccc
     3661 aatagggaaa gaaatgcgag cgcatgaacc accaaccgca tggcaatctc ccagtaggtt
     3721 tgaacgatgt aaacctttat gtgcacgaag catttatttt ggcccacacg ttaacattat
     3781 gaaacatggg gggacatagg ggcttagaca gtttgaaaat actaacacgg tccctgggct
     3841 cgccggacgc gaattctcat tgccagcctc gtcgcaagtt attcatcagc aataagatat
     3901 gactagaacg cttattttgg gttgtctttt ttggttttgg cataaaacat gcgtcagggg
     3961 ataacaccag acgtcttccc atcgatggga atgggcatgc ttgtcaggtc ggcctgcggt
     4021 gggtcggcca aggagtgtct tgtgtcggtt cctcgagtct ctagcataag gagcgacaga
     4081 gtgaaacgtg tattgtctcg tgtttggcag gaggatgaag gtgttttgac cttcgaatgg
     4141 atcctcctca tcaccgtcct ggtgatcggt gttgtcggtg gtctcagcgc ggtt
//
LOCUS       DAOPTR010000221         4186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_17110_length_4186_cov_3.829823, whole genome shotgun sequence.
ACCESSION   DAOPTR010000221 DAOPTR010000000
VERSION     DAOPTR010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17110_length_4186_cov_3.829823"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="PKI05_05505"
     CDS             <1..839
                     /locus_tag="PKI05_05505"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOA51692.1"
                     /translation="PLPVQTVKEFVRGVKRCVVIEEGDPVIEELCLTHGIPVEGKPEE
                     DRFGELSVSRVRRILNGDCPPPPPPASTRPPQLCPGCPHRMVFEALRDLGCIVAGDIG
                     CYTLGVLPPFEAMDTCTCMGASIGVGLGLRHVLPPDQARRVVSVIGDSTFVHSGLTGI
                     VEMMYNPPPGGHVVLILDNRTTAMTGLQDHPATGRTLSGQPTHELSLEGVCRAIGVED
                     VTVFEEHSNKESFRALLREKLSQAKPCVIIVRRECLLAMRRRKQESLAAQQERVTCQP
                     SE"
     gene            821..1321
                     /locus_tag="PKI05_05510"
     CDS             821..1321
                     /locus_tag="PKI05_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOA51693.1"
                     /translation="MSTERVTNIVVAGVGGQGVLRATDVIAETAFLAGYDVKKTEVHG
                     MSQRGGGVVSDVRFGPRVLSPMVPDGEADFLLVLEPTQLEPNRHRLKPDGVIIGCDRI
                     NVASLTHPKTLNVALLGVLSQYLDLSCELWCDALRRCFPPHLYSVNEEAFELGRQADG
                     KACRRE"
     gene            1334..2677
                     /locus_tag="PKI05_05515"
     CDS             1334..2677
                     /locus_tag="PKI05_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOA51694.1"
                     /translation="MSSNLESWSDGDSPVHPLSAVDFLPRPQLQAMQLYRLQGIVRWT
                     YERVPLFRQRMDERGLKPKDIRELQDIEKLPFSQKADLHACYPTGLFACSMQEIVRLH
                     ASSGTKSKPTVVAYTQKDLDLWASVMARTFLLCGIRRGDIIQNAYGYGLFTGGLGFHS
                     GAEALGATVIPISTGNSDRQIMIMKDFGCTAICCTPSYFLHLIERARDLGVDLKKHPV
                     RVGIFGAEPWTEAMRQRIQEEAGIKAHDCYGLSEIIGPGVAAECPVQQGLHIFEDHFY
                     PEIVDPNTGEVLPEGEEGELVLTTLTKQAMPMIRYRTRDITRLISAPCPCGRTIRRIA
                     RIRRRSDDMLIIRGVNVFPSQIEEALLTVEGTLPHYQIVLTREDNLDQMEVRVEVTPG
                     VFTDTVGAMEQLRQRLIHQIETTVGIRVRVRLVEPQSLQRSEGKAKRVLDLREQQ"
     gene            2709..3146
                     /locus_tag="PKI05_05520"
     CDS             2709..3146
                     /locus_tag="PKI05_05520"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOA51695.1"
                     /translation="MKIIQLSLFLENKPGQLLRICQILAQAQLNLVTLCLADTQQFGI
                     LRLILRDWQKAKELLEAHGFVATTTEVVAIEVEDRPGGMARILEILAERQINIEYLYA
                     FTFKRGDKAVVVFRFDRPDEAIAALQQSGQNVLGPVEIYGDAA"
     gene            3260..>4186
                     /locus_tag="PKI05_05525"
     CDS             3260..>4186
                     /locus_tag="PKI05_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51696.1"
                     /translation="MFRYGSAFAVLVCTLGWNIALTELPEPPGGTFRIPAGKTGDGVP
                     VIASWTDDAGPDETFLLVGAGLTDRVYVWGLDPSCPGGREIRPKVQFCTGQMLAATLP
                     ERAYDGVFVVWVENDVGRSRPIVLNAPQPWWCHPRQVQAGGTFRVFGRNLARRPHFGQ
                     SFIYLESENGSGRWLPVVKCEKYQVTAAMPKDVPAGRYRLWFHAGCGGTWGWGGPLVV
                     EVVGPKPEVPVIAFAGGDLQAAIDQLAERGGGILNLPEGVFSLRGTLVIPAGVTVWGQ
                     GRDKTILQAPSDPAIPFARFYPPAWDQGPTGVH"
BASE COUNT          855 a         1104 c         1289 g          938 t
ORIGIN      
        1 accccctgcc ggttcaaacg gtcaaggagt tcgtgagggg cgttaaacgt tgcgtggtca
       61 tcgaagaggg tgatcccgtc attgaagaat tgtgcctgac tcatggtatt cctgtggagg
      121 gcaagcccga ggaggatcgc tttggcgaac tcagtgtttc ccgtgtccgc cgtattttga
      181 atggtgactg tccgcccccg cctccacctg cttcgacccg accaccgcaa ttgtgtcccg
      241 gctgtccgca tcggatggtc tttgaggccc tccgcgacct gggttgcatt gtggccggcg
      301 acatcggttg ctacaccctg ggcgttttac ctccctttga agcaatggat acctgcacct
      361 gtatgggggc cagcatcggt gtagggctgg gactgcggca cgtgcttccg cctgatcaag
      421 cgcgtcgcgt ggttagtgta attggcgata gcacgtttgt gcacagcggg ttaaccggca
      481 ttgtggaaat gatgtataac ccgccgccgg gaggccatgt tgtcttgatt ctggacaaca
      541 gaaccaccgc catgaccggg ctccaggacc atccggcaac gggaaggacc ttgagcgggc
      601 agccgacgca tgagctgagt ttggaaggcg tctgtcgggc gatcggggtg gaggacgtca
      661 ccgtttttga agagcactcc aataaggaaa gttttcgggc acttctgcgg gagaaactgt
      721 cacaggccaa accgtgcgtc atcattgtga gacgtgaatg cctcctggcc atgaggcgac
      781 gaaaacagga atctctggcg gctcagcagg agcgtgtgac atgtcaaccg agcgagtgac
      841 caacattgtg gtggccggag tcggcggcca gggggtcctg cgggcaacgg atgtaattgc
      901 cgagacagcc tttcttgcgg gttacgacgt caaaaaaacc gaggtccacg gcatgagtca
      961 acgaggtggg ggtgtcgtga gcgatgtccg tttcggcccc cgcgtgctca gcccgatggt
     1021 cccggatgga gaggccgact tcttgctggt tttggagccg acgcaactgg aaccgaatcg
     1081 gcatcgccta aagccagatg gcgtgatcat cggctgcgat cggatcaacg ttgcatcgtt
     1141 aacccatccc aaaaccttga atgtggccct gctgggtgtt ctcagtcagt acctcgatct
     1201 gtcgtgcgaa ctgtggtgtg acgccctgcg caggtgcttt ccaccccatt tgtattccgt
     1261 caacgaggag gcatttgaat tgggacgaca agccgatggg aaggcttgcc gaagggaatg
     1321 aagagccatg acgatgagtt cgaacctgga atcctggagt gacggcgata gcccggtgca
     1381 tcccctcagt gctgttgatt tcttgccccg gccccaatta caggcgatgc aattgtacag
     1441 gttacagggg attgtccgct ggacgtacga acgcgtaccc ctgttccgtc aaaggatgga
     1501 cgaacgtggc ctgaagccta aagatattcg tgagcttcag gacatcgaga agctaccttt
     1561 tagccaaaag gcagacctcc acgcatgtta tccgaccggg ttatttgcct gttcgatgca
     1621 ggaaattgtg cggcttcacg cctcgagtgg caccaaaagc aagcccactg tggtggctta
     1681 tacacaaaag gacctggact tgtgggcaag tgtcatggcc cggacgttct tgctttgtgg
     1741 cattcgccgc ggtgacatca ttcaaaatgc ctacgggtac gggttattca cgggaggctt
     1801 gggtttccat tctggagccg aggccttggg ggccaccgtc attcctattt caacggggaa
     1861 ctcggaccgg caaatcatga tcatgaaaga cttcggttgc accgccatct gttgtacgcc
     1921 gagttacttc ctccacctga ttgaacgtgc ccgcgatctc ggcgttgatt tgaaaaagca
     1981 ccctgtgcgc gtggggatct ttggggcaga gccttggacg gaagccatgc ggcaaagaat
     2041 ccaagaggaa gcgggcatca aggcccatga ctgttatgga ctttcggaga tcattgggcc
     2101 gggcgtggcc gctgaatgtc ccgttcagca gggcttgcac atttttgagg atcatttcta
     2161 tccggagatc gttgatccta atacggggga agtccttccc gaaggagaag aaggcgaatt
     2221 ggttttgacc accctgacca agcaggccat gcctatgatt cgttatcgta cccgggatat
     2281 tacccggctg atttcggcgc catgcccgtg tggtcgcacg atccgccgta tcgcccggat
     2341 tcgacgccgt agtgatgaca tgctcatcat tcgaggcgtc aacgtctttc cgtcacaaat
     2401 tgaagaggcc cttttgacgg ttgagggaac actgccgcat tatcagattg tgctaacgcg
     2461 ggaagataat ctcgaccaga tggaagtccg tgtggaagta acaccagggg tctttaccga
     2521 taccgtgggt gcgatggaac aactgcggca gcgactgatt caccagattg aaaccactgt
     2581 gggtattcgg gtacgtgtgc gcctggttga gccgcagtcc ctacagcgaa gcgaggggaa
     2641 ggccaagcgg gtgctcgatt tgagagagca acaatgacgc cgggtgacgc aattgaagga
     2701 gcgacgtcat gaaaattatc caactctcgt tattcctgga aaataagcca ggacaacttc
     2761 tccgcatttg ccaaatcctc gcccaggccc agctcaatct ggtcacgctg tgcttagccg
     2821 atactcagca gtttggtatc ttacggctga tccttcgcga ttggcaaaag gccaaggaac
     2881 tcctcgaggc acacggtttt gtcgcgacaa cgacagaggt tgttgccatt gaagtcgagg
     2941 atcgccccgg cggcatggcc cgtattttgg aaatcctcgc cgagcgtcag atcaacatcg
     3001 agtacctgta cgcgtttacc ttcaaacggg gcgacaaggc cgtggtggta tttcgttttg
     3061 atcgccctga tgaggctatt gccgcgctcc agcagtcagg gcagaatgtc cttggacccg
     3121 tggagattta cggcgacgcg gcctgaagcc tagggaaatc ttccttgcca ttagaaacct
     3181 gcgagagaat ccttcccaac cggaagtatc cacagggaag gccagttctg gttttcgctg
     3241 gaggtggagg aaaccttcga tgttccgcta tggtagcgcg tttgcggtgt tggtctgcac
     3301 gttgggatgg aacatcgcct tgaccgagtt gcccgaaccg cctgggggga cctttcggat
     3361 tcctgcggga aagacagggg acggtgtgcc cgtgattgcc tcttggacgg acgatgctgg
     3421 gccagatgaa acatttctcc tcgtcggcgc gggcttgact gatcgcgttt atgtttgggg
     3481 gcttgacccg tcttgccccg gtggccgaga aattcgacca aaagtccaat tctgcaccgg
     3541 ccagatgttg gcggcgacgc tgccggaacg ggcttacgac ggcgtctttg tcgtgtgggt
     3601 ggaaaacgat gtcgggcgat cccgaccgat cgtcctcaac gcgccgcagc cctggtggtg
     3661 tcacccacgg caggttcagg ccggagggac cttccgcgtc tttggacgca atcttgcccg
     3721 ccgtccgcac ttcggtcaga gttttattta cctcgaatca gaaaacggct caggacggtg
     3781 gttgccggtg gtgaaatgcg agaaatacca agtgacggcc gccatgccga aggatgttcc
     3841 ggcagggcgc tatcgattgt ggtttcatgc cggttgcggg ggaacttggg gctggggtgg
     3901 cccgctggtc gtggaggttg tgggacctaa accagaggtc ccggtcattg cgttcgctgg
     3961 cggcgacttg caggcggcga tcgaccaatt ggccgagcgc gggggtggaa tcttgaactt
     4021 gcccgaaggg gtcttctcac tgcgtgggac attggtcatc ccggccgggg tcacggtttg
     4081 gggtcaagga cgcgacaaga ccattcttca ggccccatcg gatccggcta tccccttcgc
     4141 gcggttctat cctccggcat gggatcaagg gcccacaggt gttcac
//
LOCUS       DAOPTR010000222         4170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_17195_length_4170_cov_3.489429, whole genome shotgun sequence.
ACCESSION   DAOPTR010000222 DAOPTR010000000
VERSION     DAOPTR010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17195_length_4170_cov_3.489429"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4170)
                     /locus_tag="PKI05_05530"
     CDS             complement(<1..>4170)
                     /locus_tag="PKI05_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51697.1"
                     /translation="ALITIIGDARSQSGPTAFGEWRASGLQLVDRTAAVSETGQPTVA
                     SYRYWGTADGGPASIFRLELVSHETGIPGGTWASRGIVAIRLDASGLLERWHIFEMKG
                     TLQEELVFRRSVEEPLSEKMIVLVDEEQASFQTASDEKGPSLRIHIPSGRQPQRLTIW
                     ERFSRPSFFIVCPLRVRQLEPAFPVKEWQTSVWLPPVYEALRVSEFHDWGVYWQDWAV
                     RLFGPLKRPPDQPLFLPDRINRMIFDPSLPPPNLAMRRAIQFQEELGKHLQELAEITN
                     TSGLQSQRQTLSPASASVAEDLPAGSAMPKSTPAATKTGKSILWRDLLSDSFLDRVHN
                     LPPGIRTVAFLVDLAALKDLGIQPVDPIPLDDLGDMGQFTDPALKAEKFLTHHSLTIV
                     VTQSAVLLTSLATTASHRNEVQWTNPPIYKLDSHSQWWQEIERAVAAGDGRRYCRIRL
                     WNDLPATVQPTTNAGADPGIAFAAQGWNATAVPQAELEGNEFVTLMIFRNDTLQATRW
                     LGLLVAFFAAWLGGFQRRFRIVTASLVFAVVVLFVPPPFVPLASGFFLGSLLALAASL
                     LVGSSDVKGKGQPIDQGPSPQPQTSGGPLPAEPVPTNLGKPQPSGTSLISVGIVAIFL
                     CCFGDAKSFATSPEAPEPPPTLRSQNGSAVSHPAEDRGPSRGSTLGALSLIAQTTQLP
                     GRESSATTPPGVTAPGSATLPPPYRVFIPVDETGKPVGQEVYVPEGLYKELQKRTSPT
                     TAKPSGWHFGSATYRGTLAATPAGDRWELSQLTANFEVWVVDAPTQLQIPLGSAEAVP
                     QLTDILLDGRPIVATWDQSRLQVNLPESGRYRIQVPIALTPRIDAGTSEVRLRIPSVA
                     NTRLELAAPFAVNRIEVPSARGRVESDPESGRWTAELGAISELVIRWPASSDPLSDTG
                     PQADILYRLRLGPSQATWELRWKFHLNNAQIDQLQVLCEPSLRLDQASADSQVRIETL
                     VADSDRQILQMGFDSPLKDEGTLMAVFTEPLPTGSGGLRLPDVQLMGIRIARRTLFIQ
                     AHPGVSLDVRESSGWRLADLVTESKRWGIPPGEAGELYEYLGGPMFWGAWVQPQPVRP
                     TMDEHVQLLATQDEILMRWQARFTSSGGISGCHRVRIPEGFSVRKVAVITGATEQPYP
                     WSVSDDQTLLLLTDRLGPDSQLLIEGRLSLPLSEDWHLQRPTLADAEVQNLTVMLFRA
                     PDVFVEVTKHVGLELVSSVQQPTGDQQLGVFLNAYAARQPQAVDLTLRIQANQPQITA
                     EQFIRVTEAANRLQAEIRLDVTIINGFLQELKLDLPPQWQIGPAGGERTASPLELKTE
                     WGLTNAGPAGYETSRVKKRLTFAAPQKDTASVALLGSPTLDDHGCVPIPWIGLSEVSS
                     LRSYLIVPKRL"
BASE COUNT          909 a         1152 c         1243 g          866 t
ORIGIN      
        1 tccagacgtt tcggcacaat aaggtacgat cggagggagg aaacttctga gagaccaatc
       61 caagggatgg gcacacaacc gtggtcgtca agcgtggggc tgcctaagag cgcgacggac
      121 gctgtgtctt tttgaggggc cgcaaaggtc agtcttttct tcacgcggct tgtctcatag
      181 cccgcgggac cagcgttggt caggccccac tccgttttca attccagtgg agatgcggtc
      241 ctttcacccc cagccggccc aatttgccac tgcggcggca aatcgagttt aagctcttgc
      301 aagaacccgt taatgatcgt cacgtcgagt cgaatctctg cctggaggcg attggctgct
      361 tccgttaccc gtatgaactg ctccgctgta atttggggtt gattggcctg aatgcgcaag
      421 gtcaggtcca ctgcttgcgg ttgtcgtgcg gcataggcgt tgaggaacac gccgagttgc
      481 tggtcgccgg taggttgttg aacagaggag accagttcca gaccaacgtg cttggttact
      541 tcaacgaaga cgtcgggtgc gcggaaaagc atcacggtta ggttttgcac ttcagcgtca
      601 gccaatgtgg gccgctgaag gtgccagtcc tcactcagtg gcagggacaa acgtccttca
      661 atgagtaatt gactgtcggg accaaggcga tccgtgagaa gaaggagcgt ttgatcgtcg
      721 gaaacggacc agggataagg ttgctccgta gcgccggtaa tcacggccac cttacggaca
      781 ctgaaaccct cggggatgcg gacacggtga cagccggata ttccgccgga ggaggtaaac
      841 cgagcttgcc atcgcatgag gatctcgtct tgggtagcga gaagttgcac gtgttcatcc
      901 atggtcggcc gtacaggctg aggctggacc caagcacccc aaaacatggg accgcccagg
      961 tattcgtaga gttcgcccgc ctcgcctgga ggtattcccc agcgtttgct ttcggtaaca
     1021 agatcggcca agcgccagcc cgatgactca cgtacatcaa gagaaacacc aggatgggcc
     1081 tggatgaaga gggttcttct cgcaatccta atccccatca attggacatc gggaagtcgc
     1141 aagccacctg agcctgtggg aagcggttcc gtaaatacgg ccattaaggt gccctcatcc
     1201 ttgagaggtg aatcaaatcc catttggagg atttggcgat cactgtcagc gacgagagtt
     1261 tcaatacgca cttggctatc agcagaggcc tgatcgagac gcaaacttgg ttcacacaat
     1321 acctggagtt gatcgatttg ggcgttattc aaatggaact tccatcgcaa ttcccaggtt
     1381 gcctgggacg ggcccaatcg caacctgtac aggatgtcgg cctggggacc tgtgtcggag
     1441 aggggatcac tactggccgg ccagcgaatg acaagttccg agattgcgcc caactcggcg
     1501 gtccatcgcc cgctttccgg gtcgctttcc acacgtcccc gagcggaggg aacttcgata
     1561 cgattgacgg cgaagggagc cgccagttca agccgcgtgt ttgcaaccga cggaatccgc
     1621 aagcggacct cgctggtccc tgcatcgatt cgcggtgtta gcgcgatggg gacctggatt
     1681 cggtaccgtc cactttctgg gaggttcacc tggagccgcg attgatccca ggtggccaca
     1741 atcggccgcc catcgagcaa gatatctgtc agttgaggga cggcttccgc acttcccagg
     1801 ggtatttgga gctgcgttgg ggcatccaca acccacactt caaagttggc cgtcagttgc
     1861 gaaagttccc agcggtctcc cgccggggtg gccgcaagcg ttccacggta ggtcgcggag
     1921 ccgaaatgcc atccactggg ctttgccgtg gtgggagaag tccttttctg aagctctttg
     1981 taaagccctt cggggacata gacttcctgt cctaccggtt ttcccgtttc atcaaccgga
     2041 ataaaaacgc gataaggagg cggaagcgta gctgaaccgg gggcagtgac accaggggga
     2101 gttgtggccg acgactcgcg gccaggaagc tgcgtcgttt gtgcgatcag cgagagggcc
     2161 cccaacgtgc ttccgcgcga tggaccgcga tcttctgcgg gatgggagac tgccgagccg
     2221 ttctgcgatc gtaacgtggg cggtggctct ggcgcctcgg gggaggtggc gaaactcttt
     2281 gcatctccga aacaacatag aaagattgcc acgatgccca cagaaatgag actcgtgcca
     2341 ctcggctggg gctttcctaa attcgttggt acgggttctg cgggtaaggg accaccgctt
     2401 gtttgcggct ggggcgaagg cccttggtca atcggttgtc ctttcccctt gacatcggaa
     2461 gacccgacca aaagcgatgc ggccaaggcc aggagactcc ctaaaaagaa ccctgaagcg
     2521 aggggaacaa aaggcggtgg gacaaagagg accacgactg cgaaaacaag gctggccgtc
     2581 acaatgcgaa agcgccgttg aaaaccgcct agccaggcgg cgaaaaaggc gacgagtaag
     2641 cccaaccagc gcgtggcttg taaagtgtca ttgcgaaaga tcatcaatgt gacaaactca
     2701 ttcccctcca gctcagcttg tgggaccgct gtggcattcc aaccttgtgc ggcaaaggcg
     2761 atgccagggt cggcaccggc attggtcgtg ggctggaccg tagccggtag gtcattccaa
     2821 aggcgtatcc gacaatagcg acggccatca ccggctgcca cggcccgttc gatttcctgc
     2881 caccattgac tgtgggaatc gagtttataa atgggcggat tggtccactg gacttcattt
     2941 cggtggctcg ccgttgtggc gagggatgtc agcaagacgg cgctttgcgt gacgacgatg
     3001 gtcaaactgt ggtgcgtaag gaatttctcg gccttcaatg cagggtcagt aaactggccc
     3061 atatcaccca aatcatccag gggaatggga tcaaccggtt gaattcccag gtccttcagg
     3121 gcagccaaat cgaccaagaa ggccaccgtc ctaattcccg gcgggaggtt atggacgcga
     3181 tcgaggaagc tatccgatag caagtcacgc cacaggatgc tcttacccgt cttagtcgcg
     3241 gcaggtgtcg atttcggcat tgcacttccc gcgggcaaat cctcggcgac cgaggcactg
     3301 gctggcgaca acgtttgccg ttggctctgt aaaccgctgg tgtttgttat ctctgcgagc
     3361 tcttgcaggt gttttccgag ctcttcttgg aattggatgg cccgcctcat ggctagattg
     3421 ggcggcggga gggagggatc aaagatcatt cggttgatac gatcaggaag aaacagtggc
     3481 tgatccggcg gacgtttcaa gggaccaaaa aggcgaactg cccagtcctg ccaatagacg
     3541 ccccaatcgt ggaactcact caccctcaga gcttcataga cgggaggaag ccagacactc
     3601 gtctgccact ccttgaccgg aaatgccggc tcgagttgcc gaactcgcag cgggcacaca
     3661 atgaaaaagg atggtcggga aaagcgttcc cagattgtca atcgctgcgg ctggcgacca
     3721 gatggaatat gtatccgcaa cgatgggcct ttctcgtcgc ttgctgtctg aaatgatgct
     3781 tgctcttcgt caacaaggac aatcattttc tcagacagcg gttcctcgac tgagcggcga
     3841 aaaaccagtt cttcctgcaa agtccctttc atctcgaaaa tgtgccatcg ttccagcagc
     3901 cccgacgcgt caagtcggat cgcaacgatc ccgcgagagg cccatgtccc tcctggaatc
     3961 ccggtttcgt gcgagaccaa ctccagccgg aaaatgctcg ccgggccgcc gtccgcagta
     4021 ccccagtagc gatatgatgc gacggtcggt tgccccgttt cactgacagc cgcggttcga
     4081 tccaccagtt gtagcccact cgcccgccat tccccgaaag cggttggccc ggattgcgag
     4141 cgcgcgtcgc caatgatggt gatgagggcc
//
LOCUS       DAOPTR010000223         4168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_17211_length_4168_cov_3.509361, whole genome shotgun sequence.
ACCESSION   DAOPTR010000223 DAOPTR010000000
VERSION     DAOPTR010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17211_length_4168_cov_3.509361"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /gene="aroB"
                     /locus_tag="PKI05_05535"
     CDS             <1..933
                     /gene="aroB"
                     /locus_tag="PKI05_05535"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOA51698.1"
                     /translation="EIFQEAGVRTDSYVVPAGEKSKGLASAESIWNWMLDVKADRGSF
                     VVALGGGVVGDLAGFAAATFARGVRLLQIPTSLLAQVDSSVGGKVGVNLPKAKNMVGA
                     FYQPCAVWIDVSTLRTLPQREYLSGLAEVVKYGVILDSNFFSFLEEHVDDLLAQDPAV
                     LVSVVKTCCQLKATIVEQDEREVTGLRAVLNYGHTFAHAIETLTGYNQWLHGEAVAIG
                     MACAAELAVRLGMFPEELAQRQESLLSSLHLPIRIRGLAADKILECMMRDKKVQHGHL
                     RFVLPTTIGRVVLVPDVSRQQVESVIDEMQDLSL"
     gene            960..2513
                     /locus_tag="PKI05_05540"
     CDS             960..2513
                     /locus_tag="PKI05_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOA51699.1"
                     /translation="MNLRLISHILGVISLIVAGFMTASLPWSLPICGQVTHFDRRGFF
                     GVLAAILVSLIVSGLLLWYGRRARSDRLLRREALAAVGLAWLVATILGALPYICSGTC
                     RGIDDSGKPIRMTVFDAIFESASGYSGTGATVLADVENPDLVPRSVLFWRSETHFLGG
                     LGIVVLFVAILGMGSAGKQLIRAEVATPTQTSTHEQTRRAAMAFGTVFVVLNLILTFL
                     LMIQGLSIYDAICHAFGTVATAGFSTYNDSIGHFKSPLIDFTVVLFMLLGCTNFGLLY
                     FAAKGDIKRLVEDVEFRTYLAICMLATISVSICLAVQHPAFSSDMGRAIRCALFQCVS
                     IQTNTGFATDDFNEWPPYAKGILLMLMYVGGCAGSTSCSVKVIRYIFLFKICALEIEQ
                     AYHPNVVRIVRVGGRPVTDADLLKSVLVYFGLVVLTTAVAWLAILAVEPSSTWGVHPD
                     RKLLDCFSAVAATLNGVGPGLGLFGPLSNYGAMHAPAKCILTLLMLLGRLEYFPLLVL
                     VMPRFWKSA"
     gene            3132..>4168
                     /locus_tag="PKI05_05545"
     CDS             3132..>4168
                     /locus_tag="PKI05_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51700.1"
                     /translation="MCANTRVWFFGLTGGAGRWLWIIASWFCQRRLGLYVPVVLTLAL
                     ASFGLLWVPGAFAVETAPAMKAGFAERDISPDLGMEQPGGYGKSYHKVFHDPCKVRAS
                     VFDDGVKQVAVVGLDVLIIREPSVKRIREDVAQRTGLDPKAILLAASHTHSGGPTGMV
                     LPGEFDHADDWVKHLAYEVASAADARYLEKVEKLTVEAICEAWEKRHPVLCGVGRGIE
                     DQVAFNRRFRMKNGLTYTHPGQLNPEIVEVAGPTDPEVTVLGVWSPDGKLEGCVVNFA
                     CHATTSPGGISANWIYYMEKAIRGFFGEHVTVVFLAGCCGDVTQVNNLSPYQNPTGEE
                     WAKLVGGRVGA"
BASE COUNT          814 a         1084 c         1236 g         1034 t
ORIGIN      
        1 gagatatttc aagaagcggg cgtccgcaca gatagctatg tggttcccgc gggcgaaaag
       61 agcaagggtc tcgcatcggc agaatctatc tggaattgga tgctcgatgt gaaggcggac
      121 cggggtagtt tcgttgttgc cctcggggga ggcgtcgtcg gtgacctggc aggctttgcg
      181 gcggcgacgt ttgcaagagg tgtccggctg ttgcagatcc cgaccagtct tctcgcgcaa
      241 gtcgatagct ctgtaggcgg caaagttggc gtaaatcttc ccaaagccaa gaacatggtc
      301 ggtgctttct atcaaccttg cgcagtatgg atcgatgtct caacgttgag aacgctcccc
      361 cagcgtgagt atctctctgg gttggctgaa gtggtcaagt atggtgttat tcttgattcc
      421 aattttttct cttttcttga agaacacgtg gatgatctgt tggcacagga ccccgccgtt
      481 ctcgtttccg ttgtgaaaac atgttgccaa ctgaaggcta cgattgtgga acaggacgaa
      541 cgcgaagtaa cggggttgcg agcggtcctc aattatggcc atacatttgc tcacgccatc
      601 gaaacattga ctgggtacaa tcaatggctt catggagagg ctgtggcgat aggcatggca
      661 tgtgcggcag aattggccgt ccggctgggg atgtttcccg aggagctggc tcaacgacag
      721 gaatcccttc tttcatcatt gcacttgccg atccggattc gcgggttggc cgcggacaag
      781 atcctggaat gtatgatgcg ggacaagaaa gtccagcatg ggcacctgcg gttcgtcctg
      841 cctacaacga ttggtcgcgt cgtgcttgtt ccggacgtgt cccggcagca ggtggagtcc
      901 gtcattgacg aaatgcaaga cctgtcgctt tgatccacag ccgatactgg ataggggtta
      961 tgaaccttcg cctcatttct cacatccttg gggttatttc attgattgtg gcgggattca
     1021 tgacggccag cttgccctgg tcgctcccca tctgtggtca agtaacacac ttcgaccgac
     1081 gaggattttt tggcgttctt gcggccatcc ttgtatcact gattgtcagc ggcttgcttc
     1141 tttggtacgg tcgtcgtgca agaagcgacc gactcctgcg gcgcgaggct ttggcggccg
     1201 tgggactagc gtggctcgtg gcaacaatcc taggggcttt accatacatc tgttcgggaa
     1261 cgtgccgagg tatcgacgat tcgggaaaac ccatccggat gaccgtcttc gacgcgatct
     1321 tcgagtcggc gtccggctac agtgggacag gggcaaccgt cctcgccgat gtcgagaatc
     1381 ctgacctcgt tccccgatcc gtgctcttct ggcgatcgga aacacatttt ttgggcggct
     1441 tggggattgt cgtcctgttc gtggccatcc tcggcatggg ctctgccggt aaacagttga
     1501 ttcgagcgga agttgccacg cctacgcaga cctccacaca cgaacaaact cgccgtgccg
     1561 cgatggcctt cgggactgtg tttgtcgtgc tcaacctgat cttgaccttc cttttgatga
     1621 tccagggatt atccatctat gacgcgatct gtcatgcgtt tggcaccgta gccaccgccg
     1681 gatttagtac ctacaacgat agcatcgggc actttaaaag ccccctaatt gacttcaccg
     1741 ttgtgctctt catgctgctg ggttgcacca actttggcct cctgtacttt gccgcaaaag
     1801 gtgacatcaa acggcttgtg gaggacgtcg agttccggac atatctagct atttgtatgc
     1861 tggctacgat ttcggtttcg atttgcctgg cggtacagca cccggcattt tcttcagaca
     1921 tgggccgtgc tatccgttgc gcccttttcc aatgtgtctc tattcaaacg aatactggtt
     1981 ttgccacaga tgacttcaac gaatggccgc cttacgccaa gggcattctc ctaatgttga
     2041 tgtacgttgg cgggtgtgcc gggagcacaa gttgcagtgt gaaggttatt cgctacatct
     2101 ttctcttcaa aatctgtgcc ctggaaattg agcaggccta tcatcccaac gtggtgcgta
     2161 ttgtgcgggt tgggggacga ccagtcaccg acgccgatct tcttaaaagt gtcctcgttt
     2221 attttggcct ggtggttctc accacggctg tggcatggct tgcgatcctc gccgtggagc
     2281 cgtccagcac ctggggcgtt catcccgatc gcaaactttt agactgtttt tccgccgtgg
     2341 cggctacact taatggcgtc ggcccgggcc tgggcctttt cggacccctg tccaattacg
     2401 gggcaatgca tgcaccggcc aaatgcatct taaccctttt gatgctcctc ggccgattgg
     2461 aatactttcc cctgctggtc ctcgtgatgc cgcgtttttg gaaaagcgcg tgaatgaaat
     2521 tcgcgcaaat accaagttgc ccgcccccac attgccgaag cagcccgaac tgagccgcgc
     2581 gccccactgt gacaagctct gtacgcaact tagagcctat cccaaagtcc ccctctccct
     2641 ctgggagagg gttagggtga gggcaagcga acgtcggagt cggtcgcgaa caccggcagc
     2701 gattcgagcc accctcaccc ccgacccctc tcccgccgag cgggcgaggg gagattatga
     2761 gataggcttt tagacaaaag gatgcctcat cggcactcga gtggccgcga tcatcgcgat
     2821 ggatcgtgtc tgcattgagc aggtttgtta tctcgtagaa agacacccct cggttgcgct
     2881 cttccttatg cactagtgtc atccttgggg gatcacgcct agtgaaaatc cctgccacac
     2941 aaatagtcgt gagcccacgg aagagtgggc aatctgatct tgtagcaaat aggttgagcg
     3001 tggtgcctgg ccgctatcaa tccctgtttg tgtggcgtat catttacaac ggctgacgac
     3061 gtgggtgacc gcggcaaagc tcgcggaatc tcaaaggtga cggtggttgt gttgctaaca
     3121 ggagtgtagc tatgtgtgcg aacacacgag tgtggttttt cgggctgacg ggcggcgctg
     3181 ggcgctggtt atggatcatc gcaagttggt tctgtcaaag acgtttggga ctttatgtgc
     3241 cggtggtgct aacgcttgct ttagcatcct tcggtctttt gtgggttccg ggggcctttg
     3301 ctgtcgagac ggctcccgca atgaaggcgg gttttgccga acgggatatt agtcccgatt
     3361 tgggaatgga gcagcctggc ggttacggca agtcgtatca caaggtgttt catgatccgt
     3421 gcaaggtccg cgcgtcggtg ttcgatgacg gggtgaaaca ggtggccgtt gtgggcctgg
     3481 atgtcctgat catccgagag ccgagtgtca aaaggattcg cgaggacgtg gcgcagcgca
     3541 cgggtctcga tcccaaggcc attctcttgg cggcgtcgca cacgcattcg ggcggcccaa
     3601 ccgggatggt cctccccggt gagtttgacc atgcggatga ttgggttaaa cacctggctt
     3661 acgaagtggc gtcggcagcc gatgccaggt atctggaaaa ggtcgaaaaa ctgaccgtgg
     3721 aggccatttg tgaggcctgg gaaaagcgac atcccgtcct ctgtggcgtg gggcgcggga
     3781 tcgaagacca ggtcgcgttc aatcggcgat tccgcatgaa gaatggtttg acctacacgc
     3841 atcccggaca actgaacccg gagattgtgg aagtcgccgg tccgaccgat cccgaggtta
     3901 cagtcctcgg tgtgtggtcg cccgacggga aactggaagg ctgcgtcgtc aattttgcct
     3961 gtcacgcaac gaccagcccg ggcgggattt ccgcgaactg gatttactat atggaaaagg
     4021 ccattcgcgg attttttgga gagcacgtga cggtggtctt tcttgcgggt tgctgtggag
     4081 atgtgacgca ggttaacaat ttgagtcctt atcaaaaccc gacgggcgaa gaatgggcga
     4141 agctcgttgg tggtcgtgtc ggggcgga
//
LOCUS       DAOPTR010000224         4168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_17213_length_4168_cov_2.983224, whole genome shotgun sequence.
ACCESSION   DAOPTR010000224 DAOPTR010000000
VERSION     DAOPTR010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17213_length_4168_cov_2.983224"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="PKI05_05550"
     CDS             <1..929
                     /locus_tag="PKI05_05550"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOA51701.1"
                     /translation="RDYALQEGIQPPPELRTAFQNLYLYGNVFGYSPRKRLDWARNLE
                     VTVRDLSKSPEPVEVLWLVGCYPSYHPRNQVVARDMARILTALGVRWGILGKEERALG
                     DCDRLFGEEGLFEMLVENNRRLFERFKFEKIVLLDPHAYRALEKFYPRFGASFPAEHY
                     TTFLASRLDQIRPWLKKPINARVTYHDNCCVGRRCLCFDPPRQLLQAIPGVELVEMKR
                     NREHALCCGGGGGGMWLDAFITEHGGRRLSDVRIQHALETGADILAVSCPFELSRFED
                     AVKVARAEDRLKVRDIIELLADSMGLGEGGGR"
     gene            926..1699
                     /locus_tag="PKI05_05555"
     CDS             926..1699
                     /locus_tag="PKI05_05555"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51702.1"
                     /translation="MNILAVVRLVPDLVEPLVVTAEEDNLNWDQTAFTLNESDDHAVE
                     EAILLKEKYGGHVSVVAFDFGDIDQVLYMLAAKGVDRVIKISWSHDEPPNSLEVARTL
                     SRLVPKLNVDLVVIGCQSHTEFLGMAGPQLAVALNWPYVGVVRGVQEGGTAGRWTAFK
                     EYPGAVLAQMEVSLPAVLGILTASQPPRYVPVSRIRAAAQSVRFETADTPAEIEEPPV
                     KIQRVYLPPAVTHAEMLTGSEAEVAQRLVDILLARGVLK"
     gene            1696..2673
                     /locus_tag="PKI05_05560"
     CDS             1696..2673
                     /locus_tag="PKI05_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOA51703.1"
                     /translation="MIRRIVVPVEVYRGHITEATREALVAARTVGNGVGEVVAYVYGE
                     PPAEGTDVLELADRIIVLEDGQVGTPVAMAQAKLLRERIASSDVDLVLVGSSSWGWDV
                     APLLAGFLSAPLVANCQVIQHAEDVLKATCRICAGKLLVDVEITNKPTVILLLPGSYA
                     HREVQPGKGVMERERVPASDWDARVEFKGWLEPEREDIDITQEEILVTVGRGIGQQEN
                     LELVEDLAKTLGGAVCGSRPVVDQGWLPPTRQVGKSGMIVKPKLYLAIGISGAPEHVE
                     GMKDAELIVAINRDEQAPIFDVAHYGIAADLFDVIPALKEALVARRQEA"
     gene            2674..>4168
                     /locus_tag="PKI05_05565"
     CDS             2674..>4168
                     /locus_tag="PKI05_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOA51704.1"
                     /translation="MVPSRPTFWNIPAWSEILQYLLGAGVVVFIVWVGIRHIRCWRKG
                     TRKPVPRLGAEVLKQALERLARFVLTPQRLQSDPYAFITHLGLFWGMVLLALGTAIAT
                     IDWDVFHLIFGVRLLQGAFYRVFELVLDVAGVALLIGLVLAVVRRYVLRPSRLTVPQF
                     FGESWESAVILGLLLLVTITGFLIEGLRIQAGLMLKSAAVIANTPENMGEEGVRDATA
                     AAAASVAPWSPVGNLVATIFRGLDASSLRQAHLVLWWIHALAAFAFLLAIPVTKANHL
                     ITAFLNVILPSSSELPTASPTIKQRIDGVLPSLTWRQRLEVSACTACGKCQEVCPAHR
                     AGYAFTPKGTVWATRGVLMREVWPWWPATAKASEEHVWLNKDAIWSCYTCRACEVQCP
                     VLVRHTNLVVSYRRPLVDESALADGLQEVLQNLQRYGNSFGQSPRKRADWTKGLPFAV
                     KNANKEAVHYLWFVGDYTSYDARAREVTQTIARIFNAAGLDYGILYEK"
BASE COUNT          869 a         1026 c         1283 g          990 t
ORIGIN      
        1 ttcgcgacta cgcattacag gaaggaattc agccgccacc tgagttacgg acggctttcc
       61 aaaacttgta tctatatggt aacgtctttg gctattctcc tcggaagcgg ctggattggg
      121 ctcggaacct cgaggttacc gttcgagatt tgtcaaagtc gccggaaccc gtcgaggtgc
      181 tatggctagt gggatgttac ccttcgtatc atccccgcaa tcaggtagtg gcgcgcgata
      241 tggcgcggat tcttacggcg ctgggtgtcc gttggggtat ccttggcaaa gaggagcgag
      301 cactgggcga ctgtgaccgg ttgtttggcg aggagggtct gttcgagatg ctggtggaaa
      361 acaaccgccg attattcgag cggttcaaat ttgaaaagat cgtgcttctt gatccccacg
      421 cctaccgtgc tttggagaag ttttatcccc gatttggggc atcgtttcct gccgagcatt
      481 acacgacgtt tctggcatct cgattggatc agatccgtcc atggttgaaa aaaccaatca
      541 atgcacgggt gacataccac gacaattgct gcgtgggtag aaggtgcctc tgcttcgatc
      601 ccccacgcca gcttttgcaa gcgattcccg gtgtagagtt ggtagaaatg aaaaggaacc
      661 gcgagcacgc actctgttgt ggtgggggcg gcggcggaat gtggttagat gcctttatca
      721 ccgaacatgg cgggcggcgg ttgtcggacg tgcgcatcca acacgccctt gaaacgggtg
      781 ccgacatatt ggccgtttcc tgtccttttg aactatcccg ttttgaagat gccgtcaagg
      841 tggcgcgtgc agaggatcgt cttaaggtac gcgacattat cgagcttttg gccgactcga
      901 tggggttggg cgaaggaggt ggaagatgaa tatcctagct gttgtacgtt tggtacctga
      961 ccttgttgag ccgcttgtgg tgacagccga ggaggacaac ttgaattggg accaaacggc
     1021 gttcactctc aacgaaagcg atgaccacgc tgtggaagaa gcaattctgc tgaaagagaa
     1081 atatggagga catgtctcgg ttgtggcgtt tgattttggg gatattgacc aggtcctcta
     1141 catgttggct gcgaaaggtg tggacagggt catcaagatc tcttggtcgc atgacgagcc
     1201 gccgaacagt ctggaagtgg caaggaccct gagtcgcttg gtccccaagc tcaatgtcga
     1261 tctcgtggtc ataggatgcc aatcccacac cgagtttctg gggatggctg ggcctcaact
     1321 agctgtagca ctgaattggc cttacgtagg tgttgtacgc ggtgtgcagg aaggcggcac
     1381 ggctgggcga tggacagcgt ttaaggagta cccaggggct gtgctggctc agatggaagt
     1441 ttcgcttccc gcggtactgg ggattttgac ggccagccag ccgccccggt atgtgccggt
     1501 aagtcgcatc cgtgctgccg ctcagagtgt cagattcgag accgccgata ctccggcgga
     1561 aattgaagag ccaccggtaa agattcagag ggtgtaccta ccgcccgcgg tcacccatgc
     1621 cgaaatgctg actggctcgg aagctgaggt cgcacaacgg ttagtcgata ttctcttggc
     1681 ccgaggagtg ctcaaatgat ccgtcgaatc gttgtaccgg ttgaagtgta tcgtgggcac
     1741 ataaccgagg caactcgcga ggcgttggtc gcggctcgca cggtgggcaa cggcgtcgga
     1801 gaggtcgtcg cttatgttta tggagaacca cctgccgagg gcacagatgt cctcgagctg
     1861 gccgaccgta tcatcgtgtt ggaagatggc caggttggga cgccggtggc catggctcag
     1921 gccaagttgc ttcgcgaaag aattgcctca tccgacgtgg acctggtttt ggtgggaagc
     1981 agtagttggg gatgggacgt tgcgcccctc ttggcgggct ttctttcggc acctttggtg
     2041 gccaattgtc aggtgataca gcatgcagaa gacgtcctca aagctacctg tcgcatttgc
     2101 gccggcaagt tgctggtgga cgtggaaatc acaaataaac cgacagtcat cctgctattg
     2161 cccggaagtt atgcgcaccg agaagtccag ccgggaaagg gcgtcatgga gcgtgaacgc
     2221 gtcccggcga gtgattggga cgcgcgcgtg gagttcaaag gatggttaga accggaacgc
     2281 gaggacattg atatcaccca ggaagagatc ctcgttacag ttggccgcgg tatcgggcaa
     2341 caggagaacc tggagttggt ggaggatttg gccaaaacct tgggtggtgc ggtgtgtggg
     2401 tcccgaccgg tggtcgatca aggctggctt ccacccacgc ggcaggtggg taagtccggc
     2461 atgatcgtaa aaccgaagct ctatttggca attggtatca gcggggcacc tgaacacgtt
     2521 gagggaatga aggacgcgga acttattgtg gctattaacc gtgatgagca ggccccgatt
     2581 ttcgatgttg cccattatgg gattgctgca gatctctttg acgttattcc tgctctcaaa
     2641 gaggcccttg ttgcgcgacg acaggaggct tagatggtgc catcgcgacc caccttctgg
     2701 aacatcccgg cctggagtga aatcctccag tatttgttag gagcaggggt ggtcgtgttc
     2761 atcgtttggg taggcatacg gcatatacgc tgctggagaa agggtaccag aaaaccagtt
     2821 ccacgattgg gcgccgaggt tctcaaacaa gcgctggagc gtcttgctcg cttcgttctg
     2881 acgccgcagc ggcttcaatc cgatccttat gcgtttataa ctcacctggg gctgttttgg
     2941 ggaatggttc ttctggcttt ggggacggca atcgccacca ttgactggga tgtgttccac
     3001 ctcatcttcg gagtacggct tttgcagggc gccttctatc gcgtgtttga attagtttta
     3061 gacgttgctg gcgtagccct gctgataggc cttgtgttag ctgtggtgcg acgttacgtg
     3121 cttcggccgt ctcgcttgac tgttcctcaa ttctttgggg aaagctggga gtcagcggta
     3181 attttgggct tgctccttct ggtgacgatc acaggatttc taatagaggg actgcgaatc
     3241 caggcgggac tcatgctgaa atcggcggcg gtgatcgcca acactccaga gaacatgggc
     3301 gaagaggggg tgcgagatgc gacggccgca gcagcggcaa gcgtggcacc gtggtcaccg
     3361 gtgggtaact tagtcgcgac gatctttcgc gggctggacg cctcatcgct gcgacaagcg
     3421 catctggtgc tgtggtggat tcatgccctt gcggcttttg catttctctt ggcaattccg
     3481 gtaacgaagg caaatcatct catcaccgct tttctcaacg tgatcctacc gagttcatca
     3541 gaactgccaa cggcatcacc gacgatcaag cagagaatcg acggggtgct tccttccttg
     3601 acgtggcggc agcgtcttga ggtcagcgcc tgcacagcgt gcggtaaatg ccaggaagtc
     3661 tgcccggcac atcgagccgg atacgccttt acgccgaaag gcacggtctg ggcgacgcgc
     3721 ggagtactga tgagggaggt ctggccatgg tggcctgcca ccgcgaaggc ctccgaagag
     3781 cacgtctggc tcaataagga cgccatttgg agctgttata cctgtcgggc atgcgaggta
     3841 caatgtcctg tccttgtgcg gcacaccaat ctggttgttt catatcgacg cccccttgtg
     3901 gatgaaagcg cccttgccga cggccttcaa gaagtccttc aaaaccttca acgttatgga
     3961 aactcattcg ggcagtcacc ccgcaaacgt gctgattgga ctaaaggttt gccctttgcg
     4021 gttaaaaacg cgaacaaaga agcggttcat tacttgtggt ttgtaggtga ttacacttcc
     4081 tatgacgccc gagccagaga agttacccag acaatcgcac gcatttttaa tgcggcgggt
     4141 ctggattacg gcatccttta tgaaaagg
//
LOCUS       DAOPTR010000225         4158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_17268_length_4158_cov_3.050207, whole genome shotgun sequence.
ACCESSION   DAOPTR010000225 DAOPTR010000000
VERSION     DAOPTR010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17268_length_4158_cov_3.050207"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            398..571
                     /locus_tag="PKI05_05570"
     CDS             398..571
                     /locus_tag="PKI05_05570"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOA51705.1"
                     /translation="MTAIVNQQTCNGCGECVQACPLDAIKIENEKAFVDPDVCGDCGA
                     CVDVCPTQSISLS"
     gene            complement(950..1651)
                     /locus_tag="PKI05_05575"
     CDS             complement(950..1651)
                     /locus_tag="PKI05_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOA51706.1"
                     /translation="MRCLDVVTPFLLVLPLSIVCAQTPGEDNVPPPCFVALFNGKDLT
                     GWKGLVGNPVTRAKMTPEELKRAQQVADQRMREHWKVENGVLVFDGKGDNLCTVKDYG
                     DFELLVDWKILPHGDSGIYLRGSPQVQIWDATDHPEGSGGLYNNKIHPSKPLKCADKP
                     VGEWNRFRIRMVGERVSVWLNDELVVDNVVMENYWERDKPIYPTGPIELQNHGNTLYF
                     KNIFIRELPRDGESP"
     gene            complement(1741..3219)
                     /locus_tag="PKI05_05580"
     CDS             complement(1741..3219)
                     /locus_tag="PKI05_05580"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOA51707.1"
                     /translation="MNRSPSMRFFRRVLPGILLVSLGLRVSSQIWGGETEERPNILFA
                     IADDWGWPHAGAYGDPVVQTPTFDRIAREGVLFTRAHVIAPTCTASRGGILTGQAAHR
                     LEEGANLHSRLPAKFRCYPDILEEAGYAIGIMRKAWGPGVLEGTGRQRNPAGPQFRNF
                     EEFLKTVPDGRPFCFWFGSTDPHRPYEKGSGVASGMDLNKIRVPPFLPDTPVVRSDIA
                     DYYFEVQRFDRELGEVIKLLEATGRLDNTLIIVTGDNGMPFPKAKATCYDAGTHVPLA
                     VCWKAKVPGGRVVDDFISLTDVAPTILEAAGLKPLPEMTGKSFLSVLLSHKSGRVDPS
                     RDHVFTERERHTCTRPNNASYPIRSIVTERWHLIWNLRPHLWPAGNPPEESVGRDYGD
                     IDGSPTWTEMLQRQNDPAIRPFFLAAGGAKRPEFELFDLLADPGEMNNLAGKPEVAAI
                     EKELKEKLQAWMRDTGDPRASGDTDFWDRAPYYGDRPKPKTP"
     gene            3312..3962
                     /locus_tag="PKI05_05585"
     CDS             3312..3962
                     /locus_tag="PKI05_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51708.1"
                     /translation="MNYFAHAVRFLDRPYFLAGTSLPDWLSVVKRTVRLRRTSLTEHL
                     TSPDERTRQLVAGVVQHLEDDYRFHSAPAFSEVLVQVLRLIQPVVNTPGVSLVFLSHL
                     MVEIMFDAALLCRFPNGANAYYAALSHVDPLWIEGTAQAIVGEEVEHLGTFVRLFCRE
                     KILYDYNDDTATLRRLNQVMARLKLPPLPTALANVLPDIRSIVAAKELDLWRALDG"
     gene            complement(3959..>4158)
                     /locus_tag="PKI05_05590"
     CDS             complement(3959..>4158)
                     /locus_tag="PKI05_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51709.1"
                     /translation="QFVQKGEVFVAQSISPQQKIESKPHDSPPLVKCILLPEGCPYQG
                     QAARRETNAAKAVTLFCQVHA"
BASE COUNT          918 a         1197 c         1069 g          974 t
ORIGIN      
        1 atgtcttgat ggaggaatat gatcgtcgtc attccccgga agaggctccc ccaacatcgg
       61 ccgatgagac gaaagtgact tctcctgatc aagtaaaggg ttcgtctgaa tagggtttgg
      121 cttcgtgccg cgtccagtcg cacgcggctg tcaaatcaac atctcacttt cggacaaacg
      181 ttcgtttgtt ggccgacgaa aaaacaccgg gcctattcca tcgggactgc tgaagcgata
      241 gattaaaggt tttcaattct agggtctcga ccctttgcca accggatagc gtattaattg
      301 ggaggataac cgcgttgacg ggcttatttc ctttccgtca acgattggat acgaaacgaa
      361 acggtagatc tgggtacgat tcatttggag gactctgatg acagcaattg taaaccagca
      421 aacgtgcaac gggtgcggag agtgtgtcca ggcctgtccg ttggatgcca tcaagattga
      481 gaacgaaaag gcgttcgttg atcccgatgt ctgcggggac tgcggcgctt gtgtcgatgt
      541 gtgccccaca cagtcgatct cgctttccta attgaattgg ctccttggcg agaaagacgg
      601 cgaaacaaaa accgcgaccg aaaatcggtc gcggttttta attctcacgg tcaaccattt
      661 agagagcccc gcctacctta cgcaaccgcg acgcgcgcta gagcggaagt catcaacaca
      721 ctactgcttg atgcgcgggc ggcttctttt gccctaaaac cttccatcgc ttcgtgccaa
      781 attacccaca ttaaaagcct atcccaaagt tccctctccc ccagggagag ggttagggtg
      841 agggcaagca acggccggaa tcgatcgaca aaaccgacag cgattcgact caccctcttc
      901 ccaggcctct ctcccggcga acgggagagg agagattatg ggatgggctc tatgggcttt
      961 ctccatcgcg gggcaattct cggatgaaaa tattcttgaa gtaaagcgta ttcccgtggt
     1021 tctgcaattc gatcggtccg gtgggataga tgggcttgtc gcgttcccag tagttctcca
     1081 taaccacatt gtccacaacc aactcgtcgt ttagccaaac gctgacccgt tcgccgacca
     1141 ttcgaatacg gaagcgattc cattctccca ccggtttgtc cgcacactta agcggtttgc
     1201 tgggatgaat tttgttattg tacagtccgc cggacccctc gggatgatcc gtcgcatccc
     1261 agatttgtac ctggggggag ccgcgtagat agataccgct gtcgccgtgg ggaagaatct
     1321 tccaatccac gagcaactca aagtcaccgt agtccttgac cgtacagagg ttgtccccct
     1381 tcccgtcaaa gaccaagaca ccattttcga ccttccaatg ctcacgcatc cgctgatccg
     1441 ccacttgctg ggcccgcttc agttcctccg gtgtcatttt tgcccgggtc acggggtttc
     1501 ccactagccc cttccaaccc gtcaaatctt ttccgttaaa gagggccaca aaacatggcg
     1561 ggggcacatt gtcttcacct ggggtctgtg cacagacaat cgataacggc aaaacgagca
     1621 aaaatggcgt cacgacatcg agacatctca tcgaacatcc tcctgttacg aagtcaatat
     1681 cgctcccacg acgacgattg aaatgttaca cagttttgca tcccaaacaa accaagtgtg
     1741 tcacggagtt tttggtttgg gacggtcccc gtagtacggt gctcgatccc aaaagtcagt
     1801 gtcgcccgaa gccctaggat cgcctgtatc acgcatccaa gcttgaagtt tttcctttag
     1861 ttctttctcg atggccgcca cctccggctt cccggccaag ttattcattt cgccaggatc
     1921 agccaacaga tcgaacagct cgaattccgg ccttttggcc ccacccgctg ccaagaaaaa
     1981 cggcctaata gccggatcgt tttgccgctg gagcatctcc gtccacgtgg gagaaccatc
     2041 aatgtctcca taatcccgcc ccaccgactc ctccggcgga ttaccggcag gccaaaggtg
     2101 cggtcggagg ttccaaatca ggtgccacct ctctgtgaca atggagcgga tcggatagct
     2161 cgcattgttg ggccgcgtac aggtgtgtcg ctctcgctcg gtaaacacat gatcgcggct
     2221 cgggtcaacc cgacctgact tgtgggaaag caagacgcta aggaaactct tgcccgtcat
     2281 ttcgggcaag ggtttcagcc ccgcggcttc caggattgtc ggtgcgacgt cggtcaaact
     2341 gatgaagtca tccaccactc gccctccggg gacctttgcc ttccaacaga ccgccaatgg
     2401 aacgtgcgtt cccgcgtcgt agcacgtcgc ctttgctttc gggaatggca tgccgttgtc
     2461 gcccgtaacg atgattaacg tgttgtccag tcttcccgtc gcttccagaa gtttaatcac
     2521 ttcgcccaac tcgcggtcaa agcgttggac ctcaaagtaa tagtcggcaa tgtcgcttcg
     2581 tacaacaggg gtatcaggca aaaacggcgg cacacgaatc ttgttcaggt ccatgcccga
     2641 agcaacacca ctgccttttt cataaggtcg atgaggatca gtggacccga accaaaagca
     2701 gaacgggcga ccgtctggca cggtttttaa aaactcctcg aaattgcgaa actgagggcc
     2761 agcaggatta cgttgccttc cggtgccctc aaggacaccc ggtccccatg ccttgcgcat
     2821 gatgccgatc gcatagcccg cttcctccaa aatatccgga tagcagcgga acttggccgg
     2881 caagcggctg tgaaggttgg caccttcctc gagtcgatgg gcggcctgac cggtgagaat
     2941 ccccccgcgc gaggccgtgc aggttggcgc aatgacgtgt gcccgtgtaa aaagcacacc
     3001 ctcgcgggca atgcggtcga aggtgggcgt ttgcaccact ggatcaccat aagcacccgc
     3061 atgaggccaa ccccaatcat cggcaatggc aaataagatg ttcggccttt cctccgtttc
     3121 cccaccccat atctggcttg agactctcag ccccaggctc acgagcaaaa tgcccggcaa
     3181 aaccctacga aagaaccgca ttgacggtga ccgattcatt tttcagcctc ccacctattg
     3241 gttagccacg ccaagcgatt aacctaagcg aatatggcca gttccaaatc cgagcgtaac
     3301 atttctctgc gatgaactat tttgcccatg ctgtgcgatt tctcgatcgt ccgtacttcc
     3361 tggcgggcac ctcccttccc gactggttgt ccgtcgtgaa acgcacggta cgactgcggc
     3421 gaacctcgtt aaccgagcac ctcacctcac cggatgagcg cacccgacaa ctcgtcgccg
     3481 gtgttgtgca acatctcgaa gacgactatc gtttccatag tgcgccggcg ttttcggaag
     3541 tcctggttca ggttttacga ctaatccaac ccgttgtcaa cacgccgggc gtgtccttag
     3601 tgtttctgtc tcatctgatg gtcgagatca tgtttgatgc tgcccttctc tgccgtttcc
     3661 ccaacggggc caatgcttac tatgcggccc tttctcatgt cgatccattg tggatcgaag
     3721 gaacagccca ggccattgtg ggggaggagg ttgaacatct cggcacattt gtgcggttgt
     3781 tttgccggga aaagattctt tacgattaca acgatgacac tgcgaccttg cgacgtctga
     3841 accaggtcat ggcccgattg aaactcccgc cgttgcctac tgcccttgcc aacgttttgc
     3901 ccgatatccg gtcgatcgtg gcggcgaagg agctcgatct ttggcgggcg ctagacggct
     3961 aagcgtgaac ttggcagaat agggtgacgg cttttgccgc gttcgtttcg cggcgagccg
     4021 cttggccttg gtatgggcag ccttctggga gaagtatgca tttgacaagc ggaggtgaat
     4081 catgcggttt ggactcaatc ttttgctgtg gactgatact ctgtgcgacg aatacctccc
     4141 ccttttggac gaactgaa
//
LOCUS       DAOPTR010000226         4135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_17379_length_4135_cov_3.062500, whole genome shotgun sequence.
ACCESSION   DAOPTR010000226 DAOPTR010000000
VERSION     DAOPTR010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17379_length_4135_cov_3.062500"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="PKI05_05595"
     CDS             complement(<1..834)
                     /locus_tag="PKI05_05595"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF017903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOA51710.1"
                     /translation="MSQASNVLVEKAYQLAKERYASWGIDTEKVLTRLQSISISMQCW
                     QGDDVGGFESSGGLSGGGILATGAYPGKARTPSELRADADMAFRLIPGKHRFNLHAIY
                     LESGGVRVERDEIEPNHFRGWMDWAAERGLGLDFNPTFFSHPLAASGFTLTHPDDGVR
                     RFWVRHGQACRRIGEAFGRHLGKPCVTNIWIPDGMKDLPADRKRPRERLVRSLDEILS
                     VPMDPAYHRDAVEGKLFGIGSEAYVAGSHEFYLGYAITRKTLLCLDSGHFHPTESVAD
                     KI"
     gene            1418..2614
                     /locus_tag="PKI05_05600"
     CDS             1418..2614
                     /locus_tag="PKI05_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51711.1"
                     /translation="MVRKSLDEKLARIHADRSCRDFILADAKDADMAFGLAAPGRSPE
                     HHAAEAKFRTLDEYRQIIREIVAQGLVDIMLMSASTNELLTIRERIFDQSHVTPAVRA
                     NDATDIWLAGGGLGVYDRQPSRPFRTTTIDHMMCGKYECSPEERRLGADLGLYSVTFN
                     NDVELDLRTLEAYKEFRIEAEKKGFRHFLEVFSPNAPGENAPQDIPRFVNDHIVRTLA
                     GVTTAGRPLFLKIPYYGPEAMEALVRYDSSLVVGILGGSAGTTYDAFYMLWEAKKYGA
                     RVALYGRKINNAEDQLTFIKYLRLVADDEIEPAEAVRAYHSDLKKLGIKPYRSLEEDL
                     TLTPTASSYSGQSQTVGRQAAAVERGVRVTMSTWRQPEDDPDFSRMSPKEKALYNIER
                     WKRILG"
     gene            2719..4053
                     /locus_tag="PKI05_05605"
     CDS             2719..4053
                     /locus_tag="PKI05_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOA51712.1"
                     /translation="MMRQCLILAGICFLGLGLTEAARSEEGSAERPNVLLIISDDHAW
                     TDYGFMGHPQIKTPSIDKLASESLVFTRGYVPSSLCRPSLATMITGLYPHQHGITGND
                     PALPDPKVNPMAGRANPQFARYYETLMERIEKVPTLPRILGEAGYVSLQTGKWWEGSY
                     RRGGFTEGMTHGDPRRGGRHGDEGLKIGREGLEPIFSFVRRAKGEGKPFFVWYAPMMP
                     HSPHTPPQEILEKYLALNLTPPQARYYAMCEWFDATIGQLLSFLETEGLRENTIIVYL
                     ADNGWIQDPAQPNRFAPKSKRSPYDGGVRTPIIFSWPGRIKPQRDDSSLVSSIDIMPT
                     LLHLCDIPCPQGLPGLNVLDREALSKRRAVFGEVYDHNVADVDHPTKSLECRWVIQDQ
                     WKLIVPADSAAAPELYQIIDDPHETVDLSKQHPELVKQLKELLDAWWPGDSR"
BASE COUNT          986 a         1085 c         1156 g          908 t
ORIGIN      
        1 aatcttgtca gcgacgcttt ccgtgggatg aaaatgtccg gagtccaagc agaggagtgt
       61 cttccgggtg atagcatacc ctaggtaaaa ctcgtgcgac ccagcgacat aggcctccga
      121 accgatgcca aacagtttcc cttcaacggc atcgcggtgg taggcaggat ccatgggaac
      181 ggacaggatc tcatcgaggg accgcaccag ccgttccctt ggccgttttc tatcggcagg
      241 caagtccttc atgccatccg gaatccaaat gttcgtaacg catggcttgc caaggtgcct
      301 tccaaatgcc tcgccgatcc ggcggcaagc ctgcccatga cggacccaaa atcgccgcac
      361 gccatcatcc gggtgggtaa gagtgaatcc actggctgcc aatgggtgtg aaaagaaggt
      421 gggattgaag tcaagcccca aaccgcgttc tgccgcccaa tccatccatc cgcggaaatg
      481 gttcggttca atctcgtccc tttctacccg cacacccccc gattcgagat aaatcgcatg
      541 gaggttaaac cggtgtttgc ctggaatcaa ccgaaaggcc atgtcggcgt cagcacgcag
      601 ctccgagggc gtgcgggcct tgccgggata agctcctgtg gccaggattc ccccgcccga
      661 gagtccgcct gaactctcaa atccacccac gtcatcccct tgccaacact gcatggaaat
      721 ggagatggat tgtaggcgag taaggacctt ttcggtatcg attccccagg aggcatatcg
      781 ttctttggct aactgataag ccttttctac gagaacattc gacgcttggc tcatgacccg
      841 ttcctcacaa cgtctccggt cccgtgacaa aagaattagc attcgactga caaaaagatg
      901 tgattattgt gatacatcgg atgccgagga accagggggt ctctgaacga gcttcccaag
      961 gaggaacgcg gtttcggatc ggatagcaga ttcaaattcc ttcttccttt cgcgaaaaca
     1021 aactgttcgt ggcgtggcag gcaagtgcgg ttagaaccga tgatgtggat ttccattgaa
     1081 atgacggttg tgcggatggt tccaaaatgt tttcgcggcg cggagaattt cagtgggttt
     1141 gggttggcaa aaggcctttc actaccgacc gcatcctcaa atccaagggg tcgtgatgac
     1201 ctggagagtc tcaggtcggt gaccggaggg ggtaatcctt cggcagtcaa gtgagtgtga
     1261 tgcaacacgc ggcgccagtt gaggaaactt gacaggccga ccgttatggt cgatggttaa
     1321 gaagggtttt gtgtaaaatg tcgattgtga acacaaacgg acatacgcgg ctttgattcc
     1381 aaggccggca gatcaagcct cttgggaggc acatgtcatg gtgagaaaat ccctggacga
     1441 aaaactggct cgaattcatg cggaccgttc ctgccgggat ttcatcttgg cagatgccaa
     1501 agacgccgat atggcttttg gtctggcagc cccgggacgc agtccggaac accatgccgc
     1561 agaggcgaag tttcgaacac tcgacgagta tcgccaaatc attcgggaaa ttgtggcgca
     1621 gggcttggtc gatatcatgc tgatgagcgc cagcaccaat gaactgctga cgatacggga
     1681 aaggattttc gatcaaagcc acgtcactcc agcagtccgg gcgaacgatg cgaccgacat
     1741 ttggctcgct gggggtgggt tgggcgtcta tgaccgacaa ccatcgcggc cgtttcgcac
     1801 gaccacaatc gatcacatga tgtgcggaaa gtatgagtgc agcccggagg aaagaagact
     1861 cggggcggac ctggggcttt attcggtcac atttaacaac gacgtcgaac ttgatttgag
     1921 aacgctggag gcgtacaagg agtttcgcat cgaagccgaa aagaagggat tccggcattt
     1981 tctcgaagta ttcagcccaa acgcaccggg cgaaaatgct ccccaagaca tcccgcggtt
     2041 tgtcaatgac catattgtgc gaactctcgc cggcgtcaca actgctgggc gtcccctttt
     2101 tctgaaaatc ccttactatg ggccagaagc tatggaggcc ttggtccggt atgatagctc
     2161 gctcgtggtc ggtatccttg gtggatcggc gggaaccaca tacgatgcgt tctacatgct
     2221 gtgggaagcc aaaaagtacg gggcacgggt ggccctttac ggtcgaaaga tcaataatgc
     2281 ggaagaccaa ctgacgttta tcaagtacct tcgtctggtt gcagacgatg aaattgaacc
     2341 cgcagaggct gtgcgagcgt atcacagcga cttgaagaag ttgggtatca agccgtaccg
     2401 ctcgctagag gaggatttga cgctaactcc gacagccagc agttattcgg ggcaaagtca
     2461 aacagttggt cgtcaggcag cagcggttga acgaggggtt cgcgtcacga tgtccacgtg
     2521 gcggcagccg gaggatgatc ctgacttcag ccggatgtct cctaaagaga aggcccttta
     2581 caatattgaa cgatggaaac ggatcttggg atgatgcccc gtgggcagca attgacctga
     2641 cctgggttct gcagagaaac ccaccatgtg tccccagagt ggcgtaggaa tgtcttttaa
     2701 ccgtgtggga ggtattttat gatgcgacag tgcttgattc tggcgggaat atgtttcttg
     2761 ggattgggtt taacggaggc tgcccgatct gaagagggtt cggccgagcg tcctaatgtc
     2821 ttgctcatca tttccgacga ccacgcctgg acggactatg gtttcatggg gcacccacag
     2881 atcaaaacac cctcaatcga caagttggcc agcgagtccc tggtatttac ccgtgggtac
     2941 gtgcccagct cgctatgccg tccctcgcta gcaacaatga tcacggggct ttacccccat
     3001 caacatggta ttacagggaa tgatcctgct ctgcctgatc ctaaggtgaa tcccatggca
     3061 ggacgggcca atccacagtt cgcaaggtat tatgaaacgc ttatggaaag gatagaaaag
     3121 gtacccacgt tgccccgaat cctgggtgag gctggttacg tctcgcttca gaccggtaaa
     3181 tggtgggaag gatcttatcg acgtggcggt tttaccgagg gtatgacgca tggtgatccc
     3241 cgtcgtggtg gtcgacacgg tgacgaaggt ctcaagatcg gccgagaggg gttggagccc
     3301 attttcagct ttgtgcgacg ggcaaagggg gaaggcaaac cgttctttgt ttggtacgca
     3361 cccatgatgc cccacagtcc acacacacca ccccaggaga ttttggagaa gtacttggca
     3421 ctaaacctca ctcctcctca agcccggtat tatgccatgt gcgaatggtt tgacgccacg
     3481 atcggacaat tgctaagctt tttggaaacc gagggccttc gcgaaaatac catcatcgtt
     3541 tatctggcag acaacggctg gatacaggat ccagctcagc ccaatcgatt cgcgcccaaa
     3601 tctaagcgat caccttacga cggaggcgtg cgaacaccaa tcatcttttc ttggccgggg
     3661 cgaatcaagc ctcaacgaga tgactcgagc ttggtgagca gtatcgacat tatgccgacg
     3721 ttgttgcact tgtgcgacat tccatgtccc caggggcttc ccggcttgaa tgtcctcgat
     3781 cgtgaggccc tcagcaaacg ccgcgccgtg tttggtgagg tgtacgacca caacgtcgcc
     3841 gatgtcgatc acccgacgaa atcattggag tgtcggtggg ttatccaaga tcaatggaag
     3901 ctaattgtac ctgccgactc cgccgcggct cccgaactct atcagatcat cgatgatccg
     3961 cacgaaacgg tggatctttc aaagcaacat cccgaactcg tcaagcaatt aaaggagctg
     4021 cttgacgcct ggtggccagg tgattctcgt taaacgaaca ggtgtccaac gacaccccct
     4081 caaactcgcc ggtcaacggt ctcagaccgg gaccggccgc gagcacacac aaatg
//
LOCUS       DAOPTR010000227         4121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_17444_length_4121_cov_3.931136, whole genome shotgun sequence.
ACCESSION   DAOPTR010000227 DAOPTR010000000
VERSION     DAOPTR010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17444_length_4121_cov_3.931136"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PKI05_05610"
     CDS             <1..291
                     /locus_tag="PKI05_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HOA51713.1"
                     /translation="QTGWFVESLLTQTLIVHIIRTQRIPFIGSCASAPMILTTLAVMA
                     VGIWLPYSPLADVLGMVPLPATYWLWIGSFLLAYGTLTHLVKCWFFRRYGDK"
     gene            294..1088
                     /locus_tag="PKI05_05615"
     CDS             294..1088
                     /locus_tag="PKI05_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOA51714.1"
                     /translation="MDSLLDALQEGRLIELPEDDDKEEALRILARILEAIPSLPAGLD
                     IEGLVLERERTSYTSLGQGWACPHARVSFEGDLLCVVGWSAHGITYSPDDKERVSIII
                     MHLVPENQRNQYLREISLLAKAIQSDPKWREIPSAKELTEVRDRLLDLISSAKETVGP
                     DARARMIRLQRPKVEPPPITDLSNLVIEPVTLVVGPDFRPIVLTQHAGLAKYLETANG
                     VVEKIETEGVFQNGGWRIIRRSKMVFQQGRVSYDCIAITTARKTPS"
     gene            1115..3013
                     /gene="mnmG"
                     /locus_tag="PKI05_05620"
     CDS             1115..3013
                     /gene="mnmG"
                     /locus_tag="PKI05_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOA51715.1"
                     /translation="MTSVYDFDVIVVGAGHAGVEAALAAARMGANTALLTSNLDTIAK
                     MSCNPAIGGVAKGQIVREIDALGGAMGQAIDATAIQYRLLNRRKGPAMHGPRAQADKV
                     AYHQKVKQICESQPNLALRQETVESLLVEKTASGLRIVGVAVRSDAIYRAPCVILCTG
                     TFMRGLLHYGEKKMEGGRAGEEAVLGLSQSLRELGFTVLRFKTGTPPRLNGRTIDYSR
                     LEMQPGDENPVPFSFLTEKVTCEQVPCWITYTNERVHEIIRANLHRAPMFTGQIQSVG
                     PRYCPSVEIKIVRFPDRPRHQLFLEPEGRSTHEVYVNGLATSVPRDVQEAMIREIRGL
                     ERAEIMRYGYAIEYDYLPPDQLQLSLETKRVAGLFLAGQINGTTGYEEAAGQGLMAGI
                     NAVRKLLGKDPVVLARHQAYIGVMIDDLVTRGVDEPYRMFTSRAEYRLRLRHDNADRR
                     LTPLGRELGLVSDERWRRFQEKLDGIHRLRAILESVKFHGVPLIKRLAQPETTWDELV
                     QICPELAAFRQDVVEQVMYDVKYEGYLAREEAAIERQRRLAEKRIPSDINYDDVPHLR
                     AEAREKLSKIRPVDFAQAGRISGITPADLAVLAVYLEQRNRRSKAPSRELLTQSEVDQ
                     VQPGGIID"
     gene            complement(2951..>4121)
                     /locus_tag="PKI05_05625"
     CDS             complement(2951..>4121)
                     /locus_tag="PKI05_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51716.1"
                     /translation="SSSTDTLSGQSKFNQFLSSIRHATRGKIHTLLFADPIEVLRGYA
                     KIETGAAVALAILPVLGLDGLNSLGGAGSAEISGWDYVREFHVFLDNPRRGALDVPVF
                     KPANLEPEEFIPSSVITYATVSLDLPTTVSRLAKLYDGFQGDGAFERRIGGRTERFLG
                     VNLTQEFLPATTGRISLVRWVEQPVTVTSISNGLIVELKDEDVAAMIVDRIYQTRQAN
                     FEKKEFGTWQYYQSTRTRPASLPATVRFPVPCLAALGRFLIVTDSPALMQELFATTSD
                     PPRALVESLEFKVVRGRIKDLAGRDEVSMLVFQRPDEAVRVFYEGVVNQPITEAMRTS
                     AESNPLLKAFIAARERNALPPFEVLQRYYAPQGALLIDDATGLHLIDFGLRKKLP"
BASE COUNT          943 a         1148 c         1154 g          876 t
ORIGIN      
        1 caaacgggtt ggtttgttga gtcactgctg acccaaacgt taatcgtcca tattatccgc
       61 acgcaacgga ttccgtttat cgggagctgc gcttccgctc cgatgatctt gacaactctg
      121 gccgtgatgg ccgttggtat ttggctaccg tattcaccac tggccgatgt cttagggatg
      181 gtgccccttc cggcaacata ttggttatgg attggcagtt ttcttcttgc ttatggcaca
      241 ttgacgcacc tcgtcaaatg ttggtttttc cggcgatatg gagataagtg agtatggaca
      301 gtctccttga cgccctccaa gagggacgac tgatcgaact tcccgaagac gacgacaaag
      361 aggaagccct gcgaattctg gcgagaatcc tcgaggccat tccctccttg ccagccggcc
      421 ttgacatcga gggtctcgta ctggaacgcg aacgcacttc atacacctcc ctggggcagg
      481 gttgggcatg tcctcacgcg cgggtctcct ttgaaggtga cttgttgtgc gtcgttgggt
      541 ggagcgccca cgggattacc tattcccccg acgacaagga gcgcgtgtcc atcatcatca
      601 tgcaccttgt gccggagaac cagcggaacc aatatcttcg ggagatatcg ctcctcgcca
      661 aggccattca aagcgatccg aaatggcggg aaataccgtc ggccaaggaa ctcaccgagg
      721 ttcgagaccg attgctcgat ttgatcagtt ccgccaaaga gacggtgggc cccgatgccc
      781 gagcgcgaat gattcgcctc caacgtccca aagtcgaacc tcctccgatc accgatctct
      841 ccaaccttgt gatcgaaccg gtgacgctgg tcgtggggcc agattttcgc cccattgttc
      901 tgacacaaca tgccggactg gcaaaatacc tagagaccgc aaatggagtc gtggaaaaga
      961 tcgagacgga gggtgttttt caaaacggag gatggcgcat tattcgcagg agtaaaatgg
     1021 tctttcagca gggccgcgtc agttacgatt gcatcgccat cactacagcc cgcaagacac
     1081 cttcctaaga actctgagac aatcctcgta acacatgacg agtgtctacg attttgatgt
     1141 gatcgtcgtc ggtgcgggac acgccggtgt ggaggccgca ttggccgctg cacggatggg
     1201 ggctaacacc gccctcctga cgagtaatct cgacacgatc gcgaagatga gttgcaatcc
     1261 cgctatcggg ggtgtcgcca agggccagat tgtccgcgag atcgacgcac tgggaggggc
     1321 gatggggcaa gccatcgatg cgaccgccat ccagtaccgc ctactcaatc gccgcaaggg
     1381 accggcaatg catggtcctc gcgcacaggc agacaaggtc gcatatcacc agaaggtcaa
     1441 gcagatctgc gaatcccaac cgaatctggc tttacgtcaa gagacggtcg aatcgttatt
     1501 ggtggaaaag acagcgagtg ggttgcgaat cgtcggggta gcggtgcgga gtgatgcaat
     1561 ctatcgtgcc ccatgcgtga ttctgtgcac ggggacgttc atgcggggcc ttcttcatta
     1621 cggtgaaaaa aagatggaag gcggtcgcgc cggcgaggag gccgttctag gactgagtca
     1681 atcactgcga gaattgggct tcaccgtttt gcggtttaag acgggcacac cgccccggct
     1741 caacggacgg actattgact acagccgcct agaaatgcag ccgggcgacg agaatcccgt
     1801 gcctttctcg ttcctgaccg agaaagtcac gtgcgagcag gtaccgtgtt ggatcacata
     1861 cacaaacgag cgggtccacg agatcattcg cgcgaacctt catcgggccc cgatgttcac
     1921 gggacaaatt cagtctgtgg gcccgcgcta ttgtccttcc gtggaaatca aaattgtccg
     1981 ctttcccgat cggccacgtc atcaactttt cctggaaccc gaagggcgat cgacgcacga
     2041 agtctatgtg aatggattag caacgagtgt gccgcgcgac gtgcaagagg ccatgatccg
     2101 cgaaatacgc gggttggaac gggcagagat catgcgttac ggttacgcca tcgaatacga
     2161 ctatctgccg cccgaccaat tgcagctctc gcttgagaca aagcgagtcg ccggcctgtt
     2221 tttggcgggt caaattaacg ggactacagg ctatgaggag gcggcaggac aagggctcat
     2281 ggccggtatc aacgccgtgc gaaagctgct cggcaaagac cctgtcgtgc tagcgcgaca
     2341 tcaggcctac atcggtgtga tgatcgacga cctcgtgacc cgcggtgttg acgagcctta
     2401 tcggatgttc acaagtcgag ccgagtatcg actgcgattg cgtcatgaca atgccgatcg
     2461 ccgtttgacg ccattaggac gggagttggg acttgtgagc gacgagcgat ggaggcggtt
     2521 tcaggaaaaa ctcgatggta tccatcgcct gcgggctatc ttggaatcgg tgaaatttca
     2581 cggcgtgcct ctcatcaagc gactggccca gcccgaaaca acgtgggacg aactcgttca
     2641 aatctgtccc gaactagcgg catttagaca agacgtcgtc gagcaggtta tgtatgacgt
     2701 caaatacgaa ggttacctag cacgggagga agccgctatc gaacggcagc gccgtctggc
     2761 cgaaaagcgg attccttccg atatcaatta tgacgacgtt ccccatcttc gcgcggaggc
     2821 acgcgaaaaa ctctcgaaga ttcgccctgt cgatttcgcc caagccggcc gaattagtgg
     2881 aatcacccct gccgatttgg ccgtgcttgc tgtctatctt gagcaacgaa accgccgttc
     2941 gaaggcacca tcacgggagc ttcttacgca atccgaagtc gatcaggtgc aacccggtgg
     3001 catcatcgat taggagagcc ccctgtggag cgtagtagcg ctgcaagacc tcaaatggag
     3061 gaagggcatt ccgctctcgg gctgcgatga aggccttcaa aagcggattc gactcagccg
     3121 aggttcgcat tgcctccgtg atgggttgat tgaccactcc ctcgtaaaag acgcgcaccg
     3181 cttcatcagg ccgctggaac acgagcatcg aaacttcatc ccgaccggca aggtctttga
     3241 tccggcctcg aacaaccttg aattccaggg actccaccaa ggcccgcggc ggatcactgg
     3301 ttgttgcgaa taactcttgc atgagcgccg ggctatctgt cacgatcaag aagcgaccga
     3361 gcgctgccaa acacggaaca ggaaacctca ccgttgcggg tagggaggcc ggtcttgtgc
     3421 gggtggattg atagtattgc cacgtgccaa actccttttt ttcgaaattg gcttgtcgcg
     3481 tctggtagat gcgatccacg atcattgccg ccacgtcctc atccttgagc tccacgatca
     3541 atccattgga aatgctggtc accgtcaccg gctgttccac ccaacgaaca aggctaatcc
     3601 tacccgttgt ggctggcagg aattcttggg taaggttaac ccccaggaaa cgttcggttc
     3661 gcccaccaat cctacgctca aaggccccat ctccctggaa accgtcataa agtttggcca
     3721 atctggaaac cgttgttggt aagtccagcg acaccgtcgc ataggtgata acactggaag
     3781 ggataaactc ttcaggctcg agattggcag gtttgaagac cggtacgtcg agagccccac
     3841 gccggggatt gtccaaaaac acgtggaact cccgcacgta gtcccagccg gatatttctg
     3901 cgctgcctgc gccgccaagc gaattcagcc catccaatcc caacaccggc agaatcgcca
     3961 gcgcgacagc agcccccgtt tcgatcttgg cgtatccccg caggacctcg atgggatcag
     4021 caaatagtag tgtgtgaatc ttacctcggg tggcatgtct tatgctcgag agaaattgat
     4081 tgaacttgct ctgcccgctc aaagtgtctg tactggaaga c
//
LOCUS       DAOPTR010000228         4121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_17448_length_4121_cov_3.094196, whole genome shotgun sequence.
ACCESSION   DAOPTR010000228 DAOPTR010000000
VERSION     DAOPTR010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17448_length_4121_cov_3.094196"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            356..994
                     /locus_tag="PKI05_05630"
     CDS             356..994
                     /locus_tag="PKI05_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYExAFE family protein"
                     /protein_id="HOA51717.1"
                     /translation="MGVWEAGVAKRDNHYEAAFESYLRTAGIPYVAVDEARRAVMADG
                     RSLKSLDFLVAPPGPVTWLVDVKGRRFPSGLEHHQYWKNWSTQDDLESLMEWEVLFGQ
                     GFRGLFVFAFHVVGNRLPVSPEWLITFRGERYAFLGVESARYRQMCHLISPQWQTVAV
                     AVRHFRELARPIQVIFSPGADYPAFDPAGQVGKAVTGERVSVAGTSQVVGPA"
     gene            complement(1004..2338)
                     /gene="hisS"
                     /locus_tag="PKI05_05635"
     CDS             complement(1004..2338)
                     /gene="hisS"
                     /locus_tag="PKI05_05635"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOA51718.1"
                     /translation="MGTLIPARTLKGFRDYLPQMMIPRERIMEIARRVYRSYGFVPID
                     TPALEYLEILAGKGSEETDKQLYRFQDHGGRWVALRFDLTVPAARFTAQHLNELGTPF
                     KRYHIAPVWRGENTQRGRYREFVQCDFDTIGTHSLTSDIETTLVIHDLLRAIGFREFT
                     IRINNRRVLNGLLERLGLSDRAVLILRALDKWGKIGSEGVKRELEEVAGLTAEQAAEV
                     LRLTEIHGDMDEVLSRAERLVQGSAVGEEGVAELREVVRGAVAGGVARECLIVDLAIA
                     RGLDYYTGTIYETFLAALPGIGSVCSGGRYDNLAALFTTQPLPGVGASLGLDRLLAAM
                     EELGMLEAVSTTAPVFIVFFDHQHRDDYLRLAAQLRTAGLAVELYPEPKKLAQQLKYA
                     DRRGHRVAILIGEDEFRKGVCQVKDLKAGTQSEVPLDPQCSALIEHLQRLLG"
     gene            complement(2409..2999)
                     /locus_tag="PKI05_05640"
     CDS             complement(2409..2999)
                     /locus_tag="PKI05_05640"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOA51719.1"
                     /translation="MPCPEKNGWERLRDDMATNGKPIKNYWLTRTSAAVRNGRLRRQD
                     VPPGEVLCFHCPAKCCQYIALPIDRPRTWEGFDTVRWFLLHERTAVFVDDGQWYLLVY
                     LTCKALRSDGRCAIYAHRPKVCRDYGMDICEFEENAVYDQFFETAEQLEEYAEAILGP
                     RSGGSIRSPRPNPLKIVEMAEKAVAEHVTCPDGRAK"
     gene            complement(3140..>4121)
                     /locus_tag="PKI05_05645"
     CDS             complement(3140..>4121)
                     /locus_tag="PKI05_05645"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOA51720.1"
                     /translation="ADILPALGFLRQVRLWRGGPMTAVTTAPSTSSAEPSQAGTIALA
                     TPAITLADLLLAVITLVAGLLIAKNIPGLMEMVFSHRLPMEPGLRYALQTITSYCVTG
                     VALIMAAGYLGLRWSQARWLVAALGVGLGFGLQEIFANFVSGLIILFERPIRVGDLVT
                     VGEFSGRVTRIRMRATTILNFERRELIIPNKEFITGRVINWTLTDPVNRVEVAVRVAY
                     GTEPQRVMDILLETAKRNPHVMTDPAPAVVFEGFGDSSVNFRLFAYVSNYDQRLTAIN
                     SLHADVYRRLSEEGITIPFPQRDVHLFYESPMPPPSREQAQRPDQAPDEK"
BASE COUNT          839 a         1235 c         1208 g          839 t
ORIGIN      
        1 caacaaaaac tgaagttctc atctttctta ctccgcctcc gtacgagatg aagcgacctt
       61 gcccgggggt acgggaaacg ctgaaaaatc gacaacgtcc ttgcagcaca attaagcctc
      121 acgtatggta tcggcaggca atgcccgatc cttcagccaa atccttcaaa acggtcccta
      181 atcgcccgca aggtcgcccc gccgcatgat gccacaccgg ttcccgggac tccccgacat
      241 ggcccgggtt accatgtttt cctgctctcg ggcctaattt tgaaacttgc gagaggggag
      301 gaactcgggt atctttgttg aggagctgaa ggcggctcaa gcgtcgaaat taaccatggg
      361 agtctgggag gcaggagtgg ccaagcggga taaccattac gaggcggcct ttgagtctta
      421 tttgcgcact gccggcatcc cctatgtggc ggtggatgag gcccggcggg cggtcatggc
      481 cgatggccga tccctcaaaa gcctcgattt cctcgtggca ccacccggtc cggtcacctg
      541 gttggtggat gtcaaggggc gacgcttccc ctcgggcctg gagcatcatc agtattggaa
      601 aaattggtcc acccaggacg atttggaaag cctgatggaa tgggaagtcc ttttcggcca
      661 aggcttccgc ggattgtttg tgtttgcctt ccacgtcgtg ggaaatcgtc tccccgtcag
      721 cccggagtgg ttgatcacgt tccgtggtga acggtacgcc tttctggggg tggaatcggc
      781 ccggtaccgg cagatgtgcc accttatttc gccgcaatgg caaacggtgg ccgtggcggt
      841 ccgccacttt cgcgaattgg cccggccgat tcaagtgatc ttctcgccag gggccgatta
      901 cccggccttt gacccggccg ggcaggttgg aaaggccgtc acaggagaga gggtttctgt
      961 tgccgggacc tcccaagttg tcggccccgc atgagcttcc ccgttagccg agcaaccttt
     1021 ggagatgctc gatgagggcg gagcactgcg gatccagagg cacctcgctt tgtgtcccgg
     1081 cctttaaatc cttcacttgg cacacgcctt ttcggaattc gtcctcgccg atgaggatag
     1141 ccacacggtg gccgcgtcga tcggcgtact taagttgctg ggccaacttt ttcggctcgg
     1201 ggtaaagctc gacggcaagt cccgcggtgc ggagttgggc cgccagtcgc agatagtcat
     1261 ctcggtgctg gtgatcgaaa aagacgatga agaccggcgc ggtcgttgac accgcttcca
     1321 gcatccccag ctcttccatg gcggcgagga ggcgatctag acccagcgag gccccaacac
     1381 ccggtaaggg ctgcgtcgtg aacaaggccg cgaggttgtc gtagcgtcct ccggagcaga
     1441 cgctcccaat gccgggcaag gccgccagga aggtctcata gatggtcccc gtgtaataat
     1501 cgagtccccg cgcgatggcc agatcgacga tgaggcactc ccgggccacg ccacctgcga
     1561 ccgcgccgcg gacgacctca cgcaattcag cgacgccctc ttcgcccacg gcgctcccct
     1621 ggacaagacg ttccgcccgg ctgaggacct catccatgtc accgtgaatc tcggttaagc
     1681 gcaggacctc ggcggcttgt tcggcagtca gtccggccac ctcctccagt tcccgcttga
     1741 cgccctcgga accgattttc ccccatttgt ccaaagcgcg aaggatgagc accgcccggt
     1801 cacttagccc gaggcgttcc aaaaggccgt tcaacacccg gcggttattg atgcgaatcg
     1861 taaattcccg aaagccgatc gcccgcaaca ggtcgtgaat gacaagggtc gtctcgatgt
     1921 ccgaggtcaa cgagtgggtt ccgatcgtgt cgaagtcgca ctggacgaat tcacggtaac
     1981 gtcctcgttg ggtgttttca cctcgccaaa ccggggcgat gtgataccgc ttgaagggcg
     2041 ttcccaactc attgaggtgt tgcgccgtga agcgtgccgc cgggaccgtc agatcaaacc
     2101 gcagagccac ccaacggccg ccgtggtcct ggaagcggta aagctgcttg tcggtttctt
     2161 cgctcccctt gccggcgagg atttccagat actcgagggc gggcgtgtcg atcggcacaa
     2221 atccgtacga ccgatagacc cgtcgggcaa tttccatgat ccgctcccgc gggatcatca
     2281 tttgcggaag ataatcacga aaacccttca gtgttcgtgc tggaatgagt gttcccatgg
     2341 tcgtttttgc aagacttctt cacttttgag ggtcagatat gtaacgcctc agcaggtctt
     2401 ccggccagtc atttcgcccg gccatccgga caggtgacat gttccgcgac ggctttttcc
     2461 gccatctcta caatcttcaa gggattgggg cgaggcgaac ggatcgaacc accggatcgt
     2521 gggccgagaa ttgcctcggc atattcctcc agttgctcgg ccgtctcaaa aaactggtca
     2581 tagacggcgt tttcttcaaa ttcgcagatg tccattccgt agtcacggca caccttgggg
     2641 cgatgggcat agatcgcaca ccggccatcg gaccgcagcg ccttgcacgt cagataaacg
     2701 agcaaatacc actgaccgtc atcgacgaac acggccgtcc gctcatggag caagaaccac
     2761 cggacggtgt cgaacccctc ccaggtccgc ggtcgatcga tcggcagggc gatgtactgg
     2821 cagcatttag cggggcagtg aaagcacagc acctcgcctg gggggacgtc ctgccgccgc
     2881 aagcggccgt tccgcacggc tgccgacgtt cgcgtcaacc agtaattctt gatcggcttc
     2941 ccgttggtcg ccatatcgtc ccgcagtcgc tcccatccat tcttctcagg acatggcatg
     3001 aaatcatcct ggaccaaaat attaggccca ggtatcccct tgaattgact ttaccgcaat
     3061 taccggacgg cgataagggg ttgccgacag acgactcggc cttagactgg cacgcatttg
     3121 atcgaaggac ccatccgcgt tacttctcgt ctggggcctg atctggccgt tgagcctgct
     3181 cccgtgaagg cggtggcatg ggcgattcgt aaaacaggtg aacatcccgc tgcggaaatg
     3241 ggatggtgat gccttcttcg ctcagccgcc gatacacatc cgcgtggagg ctgttgatcg
     3301 cggttaggcg ttgatcgtag ttgctgacgt atgcaaacag ccggaaattg accgagctat
     3361 cgccaaaacc ctcgaagacg actgcgggcg caggatcggt catgacgtgg gggttccttt
     3421 tggcggtttc tagcaagatg tccatcaccc gctggggctc ggtcccgtag gccacacgca
     3481 ccgcgacctc aacgcgattg acggggtccg tcaaggtcca gttgatcacc cgtcccgtga
     3541 tgaattcctt attggggata atcaattccc tccgctcgaa attgaggatt gtggtggcac
     3601 gcatgcggat acgtgtcacg cggccggaaa actctccaac cgtcactagg tcacctaccc
     3661 gaatgggccg ttcaaagagg ataatcaagc cggaaacgaa gttggcgaag atctcttgga
     3721 ggccaaagcc caaccccacg cccagggcgg ccacaagcca ccgggcctga gaccatcgaa
     3781 gacccaagta cccagccgcc atgatgaggg cgaccccggt cacgcaatac gatgtgattg
     3841 tctggagggc gtatctcagc cctggctcca ttggcaaccg atgggaaaag accatctcca
     3901 tcagtccagg gatgttcttg gcaataagca atccggccac caatgttatg acggccagaa
     3961 gcagatccgc caaggtgata gccggtgtgg cgagggcgat cgtgcccgcc tgggagggct
     4021 ccgcggagct ggtcgaaggt gcagtggtca cggcagtcat tgggccaccg cgccaaagac
     4081 ggacctgcct gaggaagccc agcgcgggaa ggatgtcggc c
//
LOCUS       DAOPTR010000229         4120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_17453_length_4120_cov_3.355720, whole genome shotgun sequence.
ACCESSION   DAOPTR010000229 DAOPTR010000000
VERSION     DAOPTR010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17453_length_4120_cov_3.355720"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..3438
                     /gene="infB"
                     /locus_tag="PKI05_05650"
     CDS             196..3438
                     /gene="infB"
                     /locus_tag="PKI05_05650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOA51721.1"
                     /translation="MTQRIYSLAKELKLESKELMDICRELNLGKASALASLSDEEVHL
                     IKEHIKRARGAGARPATASTVSEPAPVFRREDYIPPAGTKPGKVPVLDRKIERPSPRP
                     EVPPSERPTPVTPTEPSPAVTATVEVSAPPTAGEVPLEPSGQVPVAPEVPPPLASETL
                     ETVVPTTGAPSAEVVEPPVAVEEKGPPRAVEVETPSVGPAVEVAELTAPEAVAAETPV
                     VAPAEVSVEGIARAEEVSPAQATAATAVSEPSTAVPEVPLVTEVSDREITPQTTQEVT
                     SEVTAVAPSSERKEEPPPPGLPPTEAERLAAIRPIPVLPRSRREAASQPGQPAEKKKK
                     TKAEPPLPRLVKLAPLPTPKPAPVPKPKEPAPQKPEIKLPVDAIRASKMGAMPLAEHL
                     LRQQEEKRKGKKKEKEETKGDISVPVLPRQKGRRTKEEELEAGEGPIGKVKRKKEAVR
                     VEDEEEAHEELEATSVQPTRLRRTRSVSTAAPRKSSVVLELPCTVRTFAEALGLPAQK
                     VLAKLFEMGTLANINATLTQEMAELLAVELGVDVVFKAETDPEEELNAIWNAPDPEDS
                     LRPRPPVVTFLGHVDHGKTSLLDKILGINVAAKEKGGITQHIRAYQIVKNGRPITFLD
                     TPGHEAFTAMRARGANCTDIVVLVVAADDGVMPQTEEAINHARAANVPIVVAINKIDL
                     PGTRAERVMQQLTGLGLVPTEWGGDIEVIRTSALTGEGIPELLDTLITLGDLYELKAN
                     YDRPACGTCLEASLQPGRGVVAKLLVQKGTLRPGDIVVCDVAHGRVKAMYDPLQPHKR
                     IQEATPATPVDLIGLDAVPEAGSRFVVLDDIAKARKIAEHRMETVHRREIMEARRHVT
                     LENLLEQLSEKEKKTLHIILRADTRGSIEAIQKEIAKLEHPEVKIQVLQALVGGITVA
                     DVHLADASDAVIIGFNVVPDEDARELAERLKVQIRRYDIIYQVADDLKAALEGLLQPE
                     KREVELGRALIQKVFHISRIGNVAGCRVLSGTITRDARLRVIRQNRIVGDYPIESLKR
                     EKDDVREVREGFECGIKLAGFDDIKEGDILEAYRIEEVKRTLETASQSTT"
     gene            3541..3972
                     /gene="rbfA"
                     /locus_tag="PKI05_05655"
     CDS             3541..3972
                     /gene="rbfA"
                     /locus_tag="PKI05_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008701349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOA51722.1"
                     /translation="MSSRRALKLAEAVRQVVSTAILFELKDPRVRDVTVTYVEVAEDL
                     SSAKVHVSVMGDEKKQQLTLRGLQNAAGFLQSRCAEKIETRWTPRLQFVLDKGVKHSI
                     EVCRILAEVLPPTPSDQAAAEITEEEQAEEGSENQDESAED"
BASE COUNT          966 a         1151 c         1258 g          745 t
ORIGIN      
        1 gccgcatggg gcgactgggc catggagggg cagcaggtcg gagaccgtga aggttgatag
       61 ttctgtcggg ttttctcacc ggtcgggaca agcggatggg cttgtctccg gctaggcgaa
      121 ggaggctgcc gatggttagt taatgtatgg gagagggttt cccgatgctg atcggtgaaa
      181 tccggagggg agtacgtgac gcaacgtatt tacagcttag ctaaagaact gaagcttgaa
      241 agcaaagagt tgatggacat ttgccgagag ttgaatctcg gcaaagcttc ggcattggcc
      301 agcctctcgg atgaagaagt ccatctcatc aaggagcaca tcaaacgggc tcgcggggca
      361 ggtgctcgcc cggccactgc ttcgaccgtc agtgaacccg ctcccgtttt taggcgagag
      421 gactatatcc caccggcagg gacaaagccg ggaaaggtgc cggttctcga ccgcaagatt
      481 gaacgcccat cgccgcggcc ggaggtgcca ccctccgagc ggcctacccc cgttacccca
      541 accgaaccct cgcccgctgt tacagcaaca gtagaggtct ccgcgcctcc caccgcaggt
      601 gaagtacctc tcgagccgtc cggtcaagtg ccggttgccc cggaggttcc acccccgttg
      661 gcgagcgaga ctctcgagac cgtggtacct accaccggtg cgccgagcgc agaggttgtg
      721 gaaccgcccg tggcagtgga ggagaagggc ccgcctcgcg ctgttgaggt ggaaaccccg
      781 agcgttggtc cagccgttga agtggccgaa ctgacagcgc cggaagcggt cgctgcggag
      841 actccggttg ttgcccccgc tgaagtttcg gtcgagggaa ttgcgcgtgc ggaggaggtc
      901 tctccagccc aggcgacggc ggcaaccgca gtaagtgaac cgtcgaccgc cgtgcccgag
      961 gtaccgttgg ttacggaggt gtcggaccgg gaaatcaccc ctcagactac ccaggaagtg
     1021 acctcagagg tgaccgctgt tgcgccctct tcggagcgga aagaagagcc accgccaccg
     1081 ggtttgccgc cgacagaggc tgagcgttta gcggccatcc gtcccattcc cgttcttccg
     1141 cgatcacggc gagaagccgc atcccagcct ggtcaaccag cagagaagaa gaaaaagacc
     1201 aaggccgagc caccgcttcc ccgattggtt aaacttgcac cacttcccac acccaagccg
     1261 gcccctgtgc ccaagcccaa ggaaccggcc ccacaaaagc ccgagatcaa gctgcccgtc
     1321 gatgccattc gggcatccaa gatgggggcc atgcccctgg cagagcactt gttgcggcag
     1381 caggaagaga agcgaaaggg caagaagaag gaaaaagagg aaaccaaggg cgatatctcc
     1441 gtgccggtct tgccccgcca aaagggacgg cggaccaaag aagaagaact tgaggctgga
     1501 gaagggccca tcggcaaggt aaaacggaag aaagaagccg tccgcgtgga ggacgaggaa
     1561 gaggcccacg aagaactgga agccacctcg gtccagccca caagattgcg gcggacgcgt
     1621 agtgtttcca cagcagcccc ccgaaaaagt agcgtcgtgt tagagctccc ttgtaccgtt
     1681 cgaacattcg ccgaggcact gggcctaccc gctcaaaagg tgcttgctaa gctctttgaa
     1741 atggggacgc tcgccaacat taacgccacc ttaacccagg aaatggcgga acttcttgcc
     1801 gtggaattgg gggttgatgt cgtcttcaag gcggaaaccg atccggagga agagctcaac
     1861 gcgatctgga atgcccctga tcctgaagat agcctccgtc cgcgaccgcc ggttgtgaca
     1921 ttcctcggcc atgtggacca cggtaagacc tcgctgctgg acaagattct cgggattaac
     1981 gtggcggcca aggaaaaggg cggcatcaca caacacattc gggcttatca aattgtcaaa
     2041 aatggtcggc ccattacctt tcttgatacc ccaggacacg aggcgttcac ggccatgcgg
     2101 gcacgcgggg ccaactgcac cgacatcgtc gtcttggtcg tagcagccga cgacggtgtc
     2161 atgccgcaga ccgaggaagc catcaatcac gcgcgtgctg ccaatgtgcc gatcgtggtg
     2221 gccatcaata agatcgatct gccggggacg agggccgagc gcgtcatgca gcagttgacc
     2281 ggcctaggtc tagtacccac ggagtgggga ggcgacatcg aggtaatccg gacaagtgcc
     2341 ttgaccggtg aaggcatccc cgagttgctc gataccctca tcacgttggg ggatctgtat
     2401 gaactaaagg ccaactacga tcgccccgcg tgcgggacgt gtctagaggc ttcgctccag
     2461 ccgggtcgcg gcgtggtggc gaaactcctg gtccaaaagg ggaccctccg gccaggtgac
     2521 atcgtcgttt gcgatgtcgc tcatggtcgg gttaaggcga tgtatgatcc cctgcaaccg
     2581 cacaaacgaa tccaggaggc aaccccggcc acaccggtcg atctgatcgg tttggatgcc
     2641 gtccctgagg cgggcagtcg cttcgtggtc ctggatgata tcgccaaagc ccggaaaatc
     2701 gccgagcatc gcatggaaac ggttcaccga cgtgaaatca tggaggcccg gaggcacgtt
     2761 acgctcgaga accttttgga acagttgagc gaaaaagaga aaaagacgct gcacatcatt
     2821 ctccgagccg atacccgggg ttcgatcgaa gccatccaga aagagatcgc caagcttgag
     2881 catcccgagg taaagatcca agttttgcag gccttagtgg gtgggatcac agtcgctgac
     2941 gtgcatctgg cggatgcctc cgatgccgtg atcattggct ttaatgtagt ccccgatgag
     3001 gatgcccgcg agttagcgga acgcctgaag gtgcaaatcc gccggtatga catcatctac
     3061 caagttgcgg acgatttgaa ggcggccttg gaaggcctgc tccagcccga aaaacgagag
     3121 gttgaactag gtcgggcctt gattcaaaag gtgtttcata ttagccgtat cggcaatgtg
     3181 gccggatgtc gggtactttc cggaaccatc acgcgcgacg cccgactccg cgtgatccgt
     3241 cagaaccgaa tcgttggcga ttacccgatc gagtccttga aacgagagaa ggacgacgtc
     3301 cgggaggtgc gagaaggatt tgaatgtggc atcaaactgg ccggtttcga cgacattaag
     3361 gagggcgata tcctggaggc ttatcggatt gaagaggtta aacggaccct ggaaacggct
     3421 agccaatcga ccacctgagt tcgaaggtcg agatgatctg tgggaaggga tgtccttctt
     3481 tcatgtggta cccatgactc gcccgcttct gtgccaggta ttttccaaga ggaggtcgtc
     3541 atgagttcgc gacgcgccct gaaactggca gaagccgtgc gccaagttgt tagtacggcg
     3601 atcctctttg agctaaaaga cccacgcgtg cgggatgtga cggtcaccta cgtggaggtc
     3661 gccgaggatc tgagctcagc caaggttcat gtttccgtca tgggagacga gaaaaaacag
     3721 cagttgaccc tccgcggctt gcagaatgca gcaggattct tgcaaagtcg ttgtgccgaa
     3781 aagatcgaga cacgctggac cccgcgtctc cagttcgtcc tcgacaaggg ggtgaaacat
     3841 tccattgaag tctgtcgcat ccttgccgag gtcttgccgc ccacgccgag tgaccaggcc
     3901 gccgcggaga tcaccgagga agaacaagca gaggagggca gcgaaaacca ggatgaatca
     3961 gcagaggatt agctcacccc gcaacaaaac catgggtcac cgattgacca gccattaaaa
     4021 acgcttaaca cgtttttgga ataaggacga ggggaccttt gattgggaga tttcccatgg
     4081 cccggcaaaa atcctcgccg aaaaggacca cgcagaaaat
//
LOCUS       DAOPTR010000230         4118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_17464_length_4118_cov_3.607433, whole genome shotgun sequence.
ACCESSION   DAOPTR010000230 DAOPTR010000000
VERSION     DAOPTR010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17464_length_4118_cov_3.607433"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2249
                     /locus_tag="PKI05_05660"
     CDS             <1..2249
                     /locus_tag="PKI05_05660"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51723.1"
                     /translation="WDRRVQTVFIHWVDAQWTLPPPAIVPAGSRHQLATSVTRLRDRS
                     PCAGWKVRYEIIGGVSALFAPNGTTSVEVPVDSTGRATAEIYQAQPLGGSTQVAVQII
                     RPADAPGAAGKQLTVGSGQTLITWSAAQLGVRVNAPTQVSIGGTTTVRVEINNPGDLP
                     AENVLLKLTLPSGVSQQSTTPAASVSGATLNWTLGRLNPHEMRTVDIVLQFSQAGTYS
                     LCAEAQAAGNLQAQHCATIAAGAGPAPGPLELRVQGPDRATVGQDVTYVIEIVNRGTT
                     PLSGLLVRDTFDAGLQHAVGQRAVERDLPGTLQPGETKTIGVLFRVVQPGRWCHTVQV
                     SGPGGIMASQTACLEAQSAGAATTTPETPPSTQPGVGAPSTGLPSTGTTPGAFPGTTT
                     LPSTPQPAVPQPTAPGLPAPPFGSESATSPSSPGGIGFQPPSFGGAPPSTPTTTPPSP
                     SAITLRLSGPTLVRLGESATFTMELVNTGNQAVSNVQLRCSFDVSWEPLRASAGYLLE
                     GETIVWTLASVEPNQTLRRQIELRAVSASQRACCRAELVEGSATLASQEACARVEPGA
                     PAPTLRERPSTLSTFGLGNGSQSSAEVAPGGIAPTPEPSSGAKETPSHAEPTRSPPQS
                     SLPVLKTWVIDRYEPITVGKTKTVTVVVENQGNNPIHGVACQVEVPPEFEVDPDQTLG
                     PAGKATVQGRKVIFDPTDVLNPTETVSFTVGLRAASPGKAHLTAKITCEGMESPMMVE
                     RVNTVIGP"
     gene            2296..2646
                     /locus_tag="PKI05_05665"
     CDS             2296..2646
                     /locus_tag="PKI05_05665"
                     /inference="COORDINATES: protein motif:HMM:NF024694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HOA51724.1"
                     /translation="MPRFRVLRHDSPRGLHYDLLLETATLLVTFALDREPTPGTTGKA
                     QQLPDHRRQYLEYEGPISGDRGSVAEWDSGLYLIMRRNEQMLDISVEGRMLKGRIVLR
                     LLDASSQEWEFQVM"
     gene            2803..>4118
                     /locus_tag="PKI05_05670"
     CDS             2803..>4118
                     /locus_tag="PKI05_05670"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOA51725.1"
                     /translation="MEPSASAESHSTASHEGTSSLALRDFLFARTNFERHPAPMGNRV
                     RVFKLARMRELLHRLGSPDRLYPIIHITGTKGKGSTAILAGTMLTASGRRVGIFTSPH
                     LEHVEERLCIDGMPCSRSRFDQLLGEIVPIVRIMDEEAAKRGESGPTYFEMLTAMAFL
                     YFAQEKVDAAVIEVGLGGRLDSTNVCHPQVCVITTVSYDHMDLLGNSLEQIAWEKAGI
                     IKRNLPVVSGVTQPGPRDVIRRVCQKRGCSLYELDRDFGVSYLPPRHLERDTALGNVT
                     FWFQGGEGNGQRTWQEGQLRLLGSHQATNAAVAIMAVRCFQQRLGWRISDQAIQVALR
                     ETIVPARVEVLGRRPVVILDVAHNTASAQALVATVRECFDVRRRYLVFGTTQGKDYRG
                     MLEVLAPHFDNIFLTQYTCNPRALPIEFLIQAAQEVIPTKFSVCPHP"
BASE COUNT          809 a         1266 c         1259 g          784 t
ORIGIN      
        1 gttgggatcg gcgggtccag acagtcttca tccactgggt tgatgcccaa tggacgctcc
       61 ccccgcccgc cattgtgccc gccggttctc gccatcagct agcgacctcg gtgacccgcc
      121 tccgcgatcg ttcgccctgc gcggggtgga aggtccgtta cgagatcatc ggtggggtca
      181 gcgcgctgtt cgcgccaaac ggaaccacga gcgtggaagt ccccgtggat tccacaggcc
      241 gcgccacggc cgaaatctat caggcccagc ccttgggggg atcaacccag gtcgcggtgc
      301 agattatccg accggccgat gcgcccggtg ccgccgggaa gcagttgacg gtaggaagtg
      361 gccagacgct catcacctgg tccgcggccc aacttggggt ccgtgtcaac gcgcccactc
      421 aggtgtctat cggcgggaca accacggtcc gggtggaaat caataatccg ggcgacctgc
      481 ccgcggaaaa cgtacttctt aagctcacgc tgcccagcgg ggtttctcag caaagcacga
      541 ctcccgccgc ctccgtcagc ggggcgaccc tcaactggac gctcgggcgt ctcaatcctc
      601 atgagatgcg aacggtggac atcgttctcc aattcagtca agcagggacc tatagcctct
      661 gcgccgaggc acaggccgcg gggaatttgc aggcccagca ctgtgcaacc atcgccgccg
      721 gggccggacc cgccccgggg cctctcgaat tgcgggtcca ggggccggac cgcgctaccg
      781 taggccagga tgtcacctac gtcatcgaga tcgtgaatcg tgggacaacg cccctgagcg
      841 gcttgctcgt gcgagacacg ttcgatgccg gactgcaaca cgccgtgggt caacgggccg
      901 ttgagcggga cctcccggga acgctccagc caggggaaac caagacgatc ggcgtgctct
      961 tccgcgtggt ccagccgggc aggtggtgcc acacggtgca agtcagtggg ccgggcggga
     1021 tcatggcctc gcagaccgcc tgcctcgaag cccagagcgc gggagcggcc acgacgacgc
     1081 cggaaacacc gccatccact cagccgggcg tcggggcgcc ttcgaccggg ctgccctcca
     1141 cggggaccac gcccggtgcg tttcccggta caaccacttt acccagcacg ccacagccag
     1201 ccgtgccgca gcccactgca ccgggtctgc cggcgccacc tttcggcagc gaatcggcca
     1261 catcgccatc atcaccaggc gggatcggtt ttcagcctcc aagttttggc ggtgcaccgc
     1321 cgtcaactcc caccaccaca cctccgtcac cttcggccat cacgttgcga ttgagtgggc
     1381 caacgctggt ccgactgggc gaatcggcaa cgtttaccat ggaactcgtc aacacgggaa
     1441 atcaagctgt cagtaacgtg caattgcggt gtagttttga tgtctcatgg gaacccctcc
     1501 gcgccagtgc cgggtatttg ttggagggcg agaccatcgt ctggacgctc gccagcgtgg
     1561 aaccgaacca gaccctgcgg cggcaaattg aactgcgtgc cgtgagcgcc tcgcagcgag
     1621 catgttgccg ggcggagttg gtcgaaggat cggccacact cgccagccag gaagcttgtg
     1681 cccgcgtgga gcctggtgcc ccagcgccca cacttcgcga acgcccctcc acgttgagca
     1741 cgttcggcct cgggaatggt tcccaatcat ccgcggaagt cgcgcccggt ggaatcgctc
     1801 caacaccgga gccgtcgagc ggagcaaagg agacgccttc tcacgcagag ccgacccgat
     1861 cgcctcccca gtcctcgctg cccgtgctga agacgtgggt tattgatcgt tacgagccca
     1921 tcaccgtggg aaagacaaag acggtcacgg tcgtcgtaga gaatcaaggg aataacccta
     1981 tccacggcgt ggcctgccag gtggaagttc ccccagagtt cgaagttgat cccgatcaga
     2041 cgctcggacc tgcgggaaag gcgacagtcc aagggcgaaa ggtgattttt gatccgacgg
     2101 atgtgctgaa tcctacggaa acggtctctt tcacagtggg actaagggct gcctcacctg
     2161 ggaaggctca tttgacggca aaaatcacct gcgagggaat ggaatcgcca atgatggtgg
     2221 aaagggtaaa caccgtcatc gggccatgaa aggcagttgc gatcctgtgg tacttccaac
     2281 gggcggaggg tggccgtgcc ccgatttcga gtcttacgac acgacagtcc gcggggactg
     2341 cattatgacc ttctcttgga aacggccaca ctgttggtga cgttcgcgct ggaccgggag
     2401 ccaactccgg ggacaacggg caaagcgcag cagttgcccg atcatcgccg acagtatctg
     2461 gaatacgaag gccctatctc cggggaccgg gggagcgtgg cggaatggga cagcgggttg
     2521 tacctcatca tgcgtcgcaa cgagcaaatg ctggacattt ccgttgaggg tcgcatgctt
     2581 aagggacgga ttgtcctccg actcctggat gcctcatcgc aggagtggga gtttcaggtg
     2641 atgtgatggc gccgaaggtg cggctttagc ccaaaagtgg ggttgcgcat cttgggacag
     2701 gtttattttt gtgagtgcaa cgttggcaaa ggtggccgtg ggtgcaacaa ttaagtatgg
     2761 tttttgagaa ctgtggccac actccagtag cgtagggcct ccatggaacc ttcagcctct
     2821 gccgaaagcc atagcacggc ctcccatgaa gggacttctt ccctcgcctt gcgggacttc
     2881 ctttttgcgc ggaccaattt tgagcgccat ccggccccga tgggcaatcg tgtccgcgtc
     2941 ttcaaactcg cccgcatgcg ggaactcctc catcggttgg gcagtcccga tcgactctat
     3001 ccgatcatcc acatcacggg gacgaagggg aaaggttcca cggcgatcct ggcagggacc
     3061 atgttgacgg cgtcagggcg gcgagtgggt atctttacct ctccccacct ggagcacgtc
     3121 gaggaacgat tgtgcatcga cggcatgccg tgttcacgct cacgatttga ccagcttctt
     3181 ggcgagatcg tccccattgt tcgcatcatg gacgaggaag cggcaaaacg tggcgaaagt
     3241 gggccaacgt actttgaaat gctcaccgcc atggcgttcc tctatttcgc ccaagaaaaa
     3301 gtcgatgccg ccgtgatcga ggttggtctg ggtggccgct tggactcgac caatgtctgc
     3361 catccgcaag tctgcgtcat caccaccgtc agttatgacc acatggacct gttaggcaat
     3421 tcgctagaac aaattgcctg ggaaaaggct gggatcatca agcgcaacct gccggtggta
     3481 agcggtgtta cccagccggg tccgcgagac gtcatccgcc gggtctgcca aaaacgaggc
     3541 tgttccttgt acgaacttga ccgagatttc ggtgtgtcgt atctgccgcc gcggcatctc
     3601 gaaagggaca ccgccttggg aaatgtcacc ttttggttcc aaggaggtga gggcaatggc
     3661 caacggacgt ggcaggaagg acagttacgg cttctgggca gtcaccaagc gaccaatgcc
     3721 gccgtggcca tcatggccgt caggtgtttc cagcagcgcc tgggatggcg catttcagac
     3781 caggccattc aggtggccct ccgcgaaacg attgtcccgg cacgggtcga ggtcctcggc
     3841 cgacgcccgg tcgtcattct ggatgtcgcc cacaatacgg cctccgcaca ggctttggtc
     3901 gccacggtgc gtgagtgctt tgatgtccgg cggcgatacc tcgtctttgg aaccacccag
     3961 ggcaaggatt accgcgggat gttggaggtg ctggccccgc acttcgataa catcttcctc
     4021 acgcaataca cttgtaatcc gcgagccttg ccaattgagt ttctcattca ggcggcccag
     4081 gaagtcatcc ccaccaagtt tagtgtgtgt cctcatcc
//
LOCUS       DAOPTR010000231         4118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_17465_length_4118_cov_3.514890, whole genome shotgun sequence.
ACCESSION   DAOPTR010000231 DAOPTR010000000
VERSION     DAOPTR010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17465_length_4118_cov_3.514890"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="PKI05_05675"
     CDS             complement(<1..1191)
                     /locus_tag="PKI05_05675"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOA51726.1"
                     /translation="MRRFEAIAFLVAGVVAVTVNAQRGVALDVIRNGRPVAVVVVETA
                     DRPSNVGRLRGDTWDDRRAAQVLVEWVEKMTGARLTVVGSVPPDQPAIVIGSVAIKAG
                     LKLDDIDSPSQEGLRISLRGDRLLLAGQSGIATTRAVCRLLESWGCRYFVDHPLGEVY
                     PKVNTLTIERLEVTEKPSFVYRSIWGSQWSGQTLWKVWNGAGGLPLQTQHAWGRYISK
                     DLFETHPEYFALRAGQRRAGDWYCTSNPEVRHLFAQGVIAEIAKGTRHPSISPPDGRG
                     YCECAACLAQDDPHLIEPSSGRVSVSTRYVDFFQDVARQVRQAAPDSILNFYCYADYT
                     QAPSRVTKLEPNLCAWLAPIRYCRLHRIGHPNCPSRMQLAQLVEAWAAIAKSLGYRTY
                     NYNLA"
     gene            1512..3482
                     /locus_tag="PKI05_05680"
     CDS             1512..3482
                     /locus_tag="PKI05_05680"
                     /inference="COORDINATES: protein motif:HMM:NF027656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOA51727.1"
                     /translation="MRFRQIPLSLMVLIVLAYIAALQGQGVAGQSVAEEPPSAPKYDT
                     YVKAPPPQEFLQRLRKTHPRLILLDEELPKIKAFLEQDVRGKAWYRNARERAEELYAR
                     PVVKHELPDGRRLLSISRDVLDRMYHWCFFYRMEKDKKWLDRAWREMEVVAEFPDWNP
                     SHYLDTAEMMHALAIGYDWLYQDLTEAQRKVIREALWRHGLRLSYSAYKGLKAEGQQS
                     WTTVTNNWNFVCNGGSSIAAMALLDEMPDECSEVLYHAFQRIQIPLQGFEPDGAWWEG
                     LGYWGYSMRYFCPYLRALETAFGTDFGFIEALKNTGFAKAGDFPVYLVSPFGTNFNFA
                     DSGSDTSHFSHWALFYLADKFENPLYQSFQEERTSGGLQDILYFRDLGKSGNLAAMPL
                     DKHFRGAELASMRSSWTDPNALFVAIKCGKNGISHAHQDLGSFIFYALGERWFIDPGT
                     ESQTYLSHQHHLPRSHFYRIREEGHNTLVFNPDEGFSQSGSGTSKIIRMVSSADEAFA
                     IADLTDAYRRHGVSVKRGYRLTQGRSVFLVQDEIESDHDNEVWWFAHSAAGTKFSLTD
                     DGRHATLERSGKTCHVYLLAPSDAVFQVMDAKPLPSSPNPSIQNPNQDMKKLAIHLPK
                     VQNVTLAVLFVPVPSGQRPSVTTLPIRPLENW"
BASE COUNT          921 a         1179 c         1137 g          881 t
ORIGIN      
        1 tgccaagttg tagttgtagg tacggtaacc caggctttta gcgatggccg cccacgcttc
       61 gacaagctgg gcgagctgca tgcggctggg acaattggga tgaccgatgc ggtgcaaccg
      121 gcaatagcgg atgggggcca accatgcaca caggttcggt tccaactttg tcacgcggga
      181 aggcgcctgc gtgtaatctg cataacagta gaagttgaga atcgaatcag gtgcggcctg
      241 acggacttgt cgagccacat cctgaaagaa atccacataa cgagtgctca ccgagactcg
      301 accactcgac ggctcaatga gatgaggatc atcttgggca aggcatgccg cgcactcgca
      361 atatcctcgg ccgtccggcg gggagatcga ggggtgccgg gtacctttgg caatctcggc
      421 aatcacccct tgagcgaaga gatgccgcac ttctgggttc gaggtgcaat accaatcccc
      481 ggcacgccgc tggccagccc gtaaggcgaa atactccgga tgggtctcaa acaagtcttt
      541 ggaaatgtaa cggccccagg cgtgctgcgt ttgcagtggt aaaccaccgg caccgttcca
      601 cactttccac agggtttggc cactccactg cgagccccaa attgaccggt aaacaaagct
      661 gggcttctcg gtgacttcaa gtctttcgat ggtcaaggta tttaccttgg gatagacctc
      721 cccgagagga tggtccacaa agtagcgaca gccccaggac tcaaggagcc gacacaccgc
      781 gcgggtggta gcaataccgc tttgcccggc taggagtaac cggtcgccgc ggagggaaat
      841 gcgtaggccc tcttgcgatg ggctatcaat gtcatcaagt ttaagcccgg ctttgatggc
      901 caccgagccg atgacaatag ccggttggtc tggcgggacc gagccaacca ccgtcaaccg
      961 cgcccctgtc atcttctcga cccattcaac cagtacctgg gccgctcggc gatcatccca
     1021 tgtgtcacct cgaaggcgtc cgacgttgct gggcctgtcg gccgtttcga ccactacgac
     1081 agccacgggg cgaccattgc ggataacatc tagcgccacc ccacgctgtg cattcacagt
     1141 aactgcgaca acgcccgcga caagaaacgc aattgcttca aaccgccgca taacagcctc
     1201 cccgtgtctt tgccaatcga taagttgaaa acttccttaa gctgactcac cataacggct
     1261 aatttatttt caccctcgca aaactaaaat atccagggcc accgactttg tcacggcgga
     1321 tggattcatt ttcgtgagaa gatccccagg ttaagatatg tgaacgattg ccttcagagc
     1381 tttccggtcc caaaatatga tgtcacaata gatgagtcgt tcccgcctga aaaagattta
     1441 acccgcttgg acacgtcgcg agacacaccc ttacctccgg tcctgcctct tgaaaagcga
     1501 aaggattgag catgagattc cgacaaatcc cgctgtcgtt gatggtcctg attgtgttgg
     1561 cctacatagc cgccttgcag gggcaaggag tggctggcca atccgttgcc gaggagcctc
     1621 ccagtgcccc caagtatgat acctacgtca aggcaccgcc cccgcaagag ttccttcaac
     1681 gattgcgaaa gactcatccg cgattgatcc ttcttgatga ggagctgccg aagatcaagg
     1741 cctttctcga gcaggacgtc cgggggaaag cgtggtaccg caacgctcgg gagcgagccg
     1801 aggaacttta cgcccggcct gtcgttaagc acgaactccc cgatggccgg agattgcttt
     1861 ccatttcgcg ggatgtgctg gaccggatgt atcactggtg cttcttctat cgcatggaaa
     1921 aagacaagaa gtggctagac cgagcatggc gggagatgga ggtcgtagcc gaatttcccg
     1981 attggaatcc ctctcattac ctggacaccg ccgaaatgat gcacgccctg gccatcggtt
     2041 atgactggct gtaccaagac ttgaccgagg cccaacgaaa ggtgatccgc gaggccttat
     2101 ggcggcatgg tctgcggctc tcatactcgg cgtacaaggg gttgaaggca gaaggccagc
     2161 agagttggac aaccgtgacc aacaactgga attttgtctg caacggtggt tcgtcgatcg
     2221 ccgccatggc cctcttggat gagatgcccg acgagtgtag cgaggtcctt tatcacgcct
     2281 tccagcgcat ccagatacca ctacaggggt ttgaacccga tggtgcgtgg tgggagggac
     2341 tcggctactg gggatattcc atgcgttact tttgccctta cttgcgggca ctagagacgg
     2401 cattcggtac cgatttcggg tttattgagg cccttaagaa cacgggattt gccaaggcgg
     2461 gcgactttcc ggtgtatctt gtctcaccgt tcgggaccaa cttcaacttt gccgattcgg
     2521 gaagtgacac ttcccatttt tcccattggg cattgttcta cctcgctgac aaatttgaaa
     2581 atccacttta tcagtccttc caagaggagc gcaccagcgg cggacttcag gatatccttt
     2641 atttccggga tttaggcaaa tcgggcaact tggccgcaat gccgttggac aagcatttcc
     2701 ggggggccga attggcctcg atgcgaagtt cctggaccga tcccaacgcc ctttttgtgg
     2761 ccatcaagtg tggtaagaac gggatttccc atgcccatca ggacttggga agtttcatct
     2821 tctatgcctt gggtgagcgc tggttcattg atcctggcac agagtcgcaa acctacctga
     2881 gtcaccagca ccatctgccg cggagtcatt tctaccgcat tcgagaggaa ggtcacaaca
     2941 cgctggtgtt taatcccgat gaaggttttt cccaaagcgg ctcaggaaca tcaaagatta
     3001 ttcgcatggt ttcttcggct gatgaggcct ttgccattgc agacctcacc gatgcctacc
     3061 gccgtcatgg agtatccgtg aaaaggggct accgcctgac tcaagggcgg agcgtctttc
     3121 tggttcaaga tgaaatcgag agtgaccacg acaacgaagt atggtggttt gcccacagtg
     3181 cagcagggac gaaattcagc ctaaccgacg acggacggca cgccactttg gagcgaagcg
     3241 gcaagacctg ccatgtctat ctgctggcgc cttcagatgc cgtctttcag gtgatggatg
     3301 cgaaacccct acccagctct cctaacccca gcatccagaa tcccaatcag gacatgaaaa
     3361 aactcgccat ccacttgccc aaggtccaga atgttacgtt agcggtgctc ttcgtgcccg
     3421 tgccgtcggg ccaacgcccg tcagtaacaa ccctgcccat tcggcccctg gaaaactggt
     3481 aagatttgcc aaggccggtt ggcgatgtat tgaggaggat cggtcatttt gtgcaagaga
     3541 gtgtcgtaaa cacaccctgc cctggcggaa acaatcacca caggcatttc ggattttatg
     3601 gctctttctc cgcatgggcc ggcttcgaga tcagcgagta aaacgatagt ttctgccaca
     3661 accccaagga ccgccgctag tacggccaca atgaggacta ttccgcgcgg cgacaatggt
     3721 acgagggcaa ccacaggggg ttgcccctac acgcgcgatc aaaaccgtag gggcaggccc
     3781 ccgtgcctgc cctgggtccc ctgtgcctgc cccagagcgt aaacagcgcc cggggcttcg
     3841 tgaaatgcaa acaacgagtt gcccacccaa acgcgaaacc tgagcgctgc gcataaacgg
     3901 gtaaccacgg cgcgtggctc ccacgagccg tttttcgcgc accgcgacac ccgtatggtg
     3961 ccccatggtt gccccgcacg atggtggagt gttcggtcgt aggggcaacc ccctgtggtt
     4021 gccccgtacg ctggttggcc cagctgatga tcgtcccgta tggtggttgc cgctgcaatc
     4081 aggttgggcg tggggggcga atgacgttcg ggtgccct
//
LOCUS       DAOPTR010000232         4118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_17466_length_4118_cov_3.191730, whole genome shotgun sequence.
ACCESSION   DAOPTR010000232 DAOPTR010000000
VERSION     DAOPTR010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17466_length_4118_cov_3.191730"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..1407
                     /locus_tag="PKI05_05685"
     CDS             109..1407
                     /locus_tag="PKI05_05685"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOA51728.1"
                     /translation="MSIDGVLGNDISRNLRTNANSAALKYLFSVESPGIHRPPSMPDR
                     EFNWLKEQVTFLPLDDCLVMHAQADLRLFDWAGTLSWSQQSGVSSDNTSRAMVAMCPL
                     LYQDRIICRRLTQRGSELVCLDLFDGHVIWAQRLGDYVVSDPWIQDEVVQAVVGTGLE
                     IENYQLTLVALDVRDGSVRQRSPLAVLNNVLDVPLDCHVVPQDRWFLIQANGCLMLFD
                     SLGRPIWLRSFPWLAPASSSWWHAQSWYTQRDPRPLIVGDHVFVSMRGGWFVACVELK
                     TGKLCWYQPMGHLRGLFAYADGKLYVETSGSMWILEATTGRKLGQYNWPARAEKAVVA
                     PTRLVMAFQSRSEERGRRHRRLVMQCIQLDDGTLLREIDLGPIDRELEWIGPVMTHHD
                     KVYILLARPNQPQQFEVYELVLAKDDPQHPPADQISALHK"
     gene            1417..2586
                     /locus_tag="PKI05_05690"
     CDS             1417..2586
                     /locus_tag="PKI05_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HOA51729.1"
                     /translation="MRWYILARKRIALLSVAVLCCLFCPTDGVFGQGEWEITPESEAA
                     LERGLAWLARNQGPEGNWDSNDLGLVSTGILAFLAAGHLPDRGPYGSTVRRALDYVIR
                     NAQPSGLLNIAEARRGTYNHGLSTFVLGQAYGMTNDRRLGPLLDQALKVVQQSQCGDG
                     GWDYVPKRQSQGHDLSLVVMQALALRSAVDSGFEISPQVVQAAIECVREHYTPKNCSR
                     DAPEEEQQRHPGQFTYSKGGGNATIAMAAAGVVCLQEFGQYDDWRIGKNMEVIRAAIL
                     EATRDGRPRREHRAPFDPYTLYYVAQALYQVGGDAWQECYPPLRDAVVASQIREPNQP
                     SRDGMWTAQGHVGGRPGELYMTAVCCFVLAIPNRYLPILQEGRIETLQKQFANAP"
     gene            complement(2824..>4118)
                     /locus_tag="PKI05_05695"
     CDS             complement(2824..>4118)
                     /locus_tag="PKI05_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA51730.1"
                     /translation="LEQFGFQAEAATRAKSAFLANMSHEIRTPMTAILGYTELLLEEL
                     QKSGASREHLEALETIQRNGNYLLKLINDILDLSKIEAGQLKVEPQSLNLPELLEDVI
                     KLMRVRAEAKALPLVLEFAGPIPKLIRSDPVRLRQILINLVGNAIKFTEEGEVRLRVR
                     LAGEQEQSQKLCIDVIDTGIGIERDKLEHIFEPFSQGDSSTTRKYGGTGLGLTISRRL
                     AQCLGGDITVVSAPGKGSVFTVTIDPGPIDECVEPNQSAMIKSGAASVEAKENPSAIR
                     LDARILLAEDAPDNQRLISFILKKAGATVTVVKDGRAAVEAAMKAWENGEPFELILMD
                     MQMPEMDGYQATRYLRDRGYSGKIVALTAHALPEDVTKALDAGCDAYLTKPIRRETFC
                     KSLSKILAQSAPDVGPSDSNMAAGAPDVEVDPGPMPVG"
BASE COUNT          875 a         1127 c         1149 g          967 t
ORIGIN      
        1 cgggatcaac tttcccaacg agcgaaagcc tgtccccaaa ttgtctcgct caatcaaagt
       61 gacgaacaaa ccctttacta aggcagggta ctcgtcacgc atgacatcgt gagcattgac
      121 ggtgtcttgg gaaacgatat ttcaagaaac ttgcggacaa acgccaactc ggcagccctg
      181 aaatatctct tctcggtgga aagtcctggc attcatcggc ccccgtcaat gccagatcgg
      241 gagtttaatt ggctaaaaga acaggtgacc tttttgcccc tggatgactg tctggtgatg
      301 cacgcgcagg cagatctccg cctcttcgat tgggctggaa cgttgagttg gtctcagcag
      361 tctggtgtgt cctccgacaa taccagtcgg gcaatggtcg ccatgtgccc acttttgtat
      421 caggaccgca tcatttgccg acgtttaacc cagcgtggtt cggaactggt ctgtttggat
      481 ctattcgatg gacatgtcat ctgggcacag cgtctcgggg actacgtcgt ctcggatcct
      541 tggattcaag acgaggtggt tcaggcggtg gttggcacgg gtttagaaat tgaaaattac
      601 cagctcaccc tagttgctct tgatgttcgt gatggtagtg ttcgccaacg gtcacctttg
      661 gctgttctga ataatgttct cgacgtaccc ctggattgcc atgttgttcc gcaagaccga
      721 tggtttctca tccaagcaaa cggttgtttg atgctttttg attctcttgg ccgtcctatt
      781 tggttgcgat cttttccctg gcttgctcca gcgtcgtcaa gctggtggca tgcccagtcc
      841 tggtacaccc aacgggatcc tcggcccctg atcgtgggcg atcacgtctt cgtcagcatg
      901 cgggggggct ggtttgtggc atgcgtggag ctaaagactg gaaagctatg ttggtatcag
      961 cccatggggc atttgcgtgg tctatttgcc tatgccgatg gcaaattata cgtcgaaacg
     1021 tccggcagta tgtggattct agaggccacg acgggacgga agctcggaca gtacaactgg
     1081 cccgccaggg cagaaaaagc cgttgtggcc ccaacacgac tggtcatggc ttttcagtcc
     1141 cgttctgaag agcgtggccg tcgccacagg cgtttggtta tgcagtgtat ccaacttgac
     1201 gatggcacac ttcttcggga gatagacctt ggcccgattg atcgggagct ggaatggatc
     1261 ggaccggtca tgacgcatca cgacaaggtg tacattctct tggcccgccc caatcaacct
     1321 cagcaatttg aggtttatga actggttcta gcgaaagacg atccgcaaca tcccccagcg
     1381 gaccaaatca gtgctttgca taagtaggat cataccatga ggtggtacat tcttgcgcga
     1441 aaacgtatcg cactcttaag tgtggccgtg ctgtgttgcc tcttttgtcc aaccgatgga
     1501 gtgtttgggc agggagaatg ggaaatcaca ccggaaagtg aagcggcact ggaacgggga
     1561 ctcgcttggt tggcacgaaa tcaagggcct gagggaaatt gggattcaaa cgatctgggg
     1621 ctggtgagta cgggtattct tgcttttttg gcggccgggc acctgcccga tcgtggccct
     1681 tacggttcca cggtccgccg tgcgctggat tatgttattc ggaatgcaca accgtctggt
     1741 ctgttgaaca tagcagaagc ccgacgggga acctacaacc atggcctttc gactttcgtt
     1801 cttggccagg cgtatgggat gaccaatgac cgacggttgg ggccactgct cgaccaggcg
     1861 ctcaaggtgg tacagcaaag tcagtgcgga gacggtggct gggattacgt acccaaacgc
     1921 cagtcccagg gacatgacct gagcctggtc gtgatgcagg cgctggcatt gaggagcgcc
     1981 gtggatagcg gttttgaaat ctctccccag gtcgttcagg ctgccattga atgtgtccgg
     2041 gagcattaca ccccaaagaa ttgcagccgt gatgcaccag aagaggagca acagcggcac
     2101 ccgggccagt tcacctacag caagggaggg ggtaatgcca ccatcgccat ggctgcggcg
     2161 ggcgttgtgt gtctccaaga gttcggacaa tacgacgact ggcggattgg caagaacatg
     2221 gaggtaatcc gcgcagctat cctagaagcg acgcgggatg gtcgtccccg acgtgaacac
     2281 cgtgctccgt ttgatcccta cacactttac tacgtggcac aggcattgta tcaggttggg
     2341 ggcgatgcgt ggcaagaatg ctatcctccc ttgcgtgacg cggttgtggc cagtcaaatt
     2401 cgcgagccga accaaccgtc acgggacggc atgtggaccg cccaaggcca cgtcgggggt
     2461 cgccccggcg aactttatat gaccgctgtc tgttgtttcg tgctcgccat tcccaaccgt
     2521 tatttgccta tccttcagga gggtagaatc gaaacactgc aaaagcagtt tgccaacgcc
     2581 ccttgaaggg aaatcgcttt cacctaaaag tctagcccac aatcaatctc ccctcgcccg
     2641 tttggcggga gagggggcgg gggtgagggt ggctcgagtc gctgccggtt ttctcaaccg
     2701 attccgacga tcgcttgccc tcaccccaaa cctctcccgg agggagaggg gaagcctttt
     2761 ggggtaggcc ttaagtcgga ttgtgcacat tgacagcgca aaccgggatg acgaagagaa
     2821 atctcagcca acgggcatgg gcccagggtc aacctcgacg tcgggagctc cagcagccat
     2881 gttcgagtcg gaaggtccga catcaggggc agactgcgca aggatcttgc tcaagctctt
     2941 acagaaggtc tcgcgacgga tcggctttgt caggtatgca tcgcaccccg cgtcaagggc
     3001 ctttgtcacg tcctcaggaa gggcatgtgc tgtcagagcc acgatttttc ccgagtaacc
     3061 gcggtccctg agatatcggg tcgcttgata gccatccatt tcgggcatct gcatatccat
     3121 taggatcagc tcaaacggct ctccattttc ccatgccttc atcgcagcct caaccgccgc
     3181 ccggccatct ttgaccacgg tgaccgtcgc tccggccttt ttcaggataa atgagatcaa
     3241 acgctgattg tccggggcat cctccgccag caagatccgc gcatctaacc gtatcgctga
     3301 ggggttctct tttgcttcca ccgatgcagc accggacttt atcattgccg attggttcgg
     3361 ctcaacgcat tcgtcaatag gaccggggtc aatggtcacg gtgaacacac tccccttgcc
     3421 gggtgcgctc accacagtga tgtcgccgcc caagcactgg gcgagacgac ggctaatggt
     3481 cagccctaac cccgtgccac catatttgcg cgttgttgac gaatcacctt gggagaacgg
     3541 ttcgaagata tgctccagct tgtcgcgctc gatacctatc cctgtatcaa tcacgtcgat
     3601 gcacagtttc tgcgattgtt cctgctcgcc ggccaaccgt acccgcaatc gaacttcccc
     3661 ttcttcggta aatttgatgg catttcccac caaattgatc aatatttgcc gcagtcggac
     3721 agggtcactg cggatcaact tgggaatcgg ccccgcgaat tcgagaacga gcggcagggc
     3781 tttggcctca gcacgcaccc gcatgagctt aatgacgtcc tctaacagct caggcaaatt
     3841 caacgactga ggctcaacct tcagttgtcc tgcctcgatt tttgacaaat ccaggatatc
     3901 gttgataagc ttcaggagat aattcccatt tcgctggatc gtctccaagg cctccaagtg
     3961 ctcgcgcgat gctcccgatt tttgaagttc ctcgagaagg agctccgtgt agcccaaaat
     4021 ggccgtcatt ggcgtccgga tctcgtgact catattggcg agaaatgcac ttttggcacg
     4081 ggtggcggcc tcggcctgaa acccaaattg ctccaggc
//
LOCUS       DAOPTR010000233         4117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_17469_length_4117_cov_2.742984, whole genome shotgun sequence.
ACCESSION   DAOPTR010000233 DAOPTR010000000
VERSION     DAOPTR010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17469_length_4117_cov_2.742984"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PKI05_05700"
     CDS             <1..617
                     /locus_tag="PKI05_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA51731.1"
                     /translation="VYHREFLVVLGPSGSGKSTLLNLLGGLDTPTEGQVLFEGRDLAE
                     FDRRELTWYRREIVGFVFQFYNLVANLTALENIMLAADLVSDPLDPVDLLEQVGLADR
                     ADSFPAQLSGGEQQRVAIARAMVKNPPLILCDEPTGALDDMTGRQILRLLLNLKEDMG
                     KTVVVITHNTAIAQMADRVIRLRSGRIVEVTEQASPLPPEKIQW"
     gene            665..2257
                     /locus_tag="PKI05_05705"
     CDS             665..2257
                     /locus_tag="PKI05_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA51732.1"
                     /translation="MNPLHRKLIRELRASGGVIFSVILITAIGTACFVAMRSAHRSLS
                     QARDHYYAKGRMADFWVSVKRMPKSELRRLSNIPGVAEVHPRIRSPVTVDLPTSEKPL
                     AGVVLSLPDRGLPALNQIQILRGEYFTSRRRNEVIVNDVFANAHRLRLGQSIFVNIAG
                     RRQELVLVGTAFSCEFIYPLGRAAIVPDRTGFGVFFLKESFAEEITDMKGATNEVLGR
                     VFPRYANSKRALLHEIERRLDEFGVLATTPLEDYPSNAFLSQEIEGLATFALLIPLIF
                     LGISAVILNVVMTRRVEQQRIAIGTLKALGYGDAVIGWHIMEFALLAGVIGAAIGCGV
                     GQWLSAGMTSIYRQFYEFPRLDAPIYWDIHLEAILVGLICAAIGGWRGARTALALPPA
                     AAMRPRVPASGRHILLERFGWLWRHVHPAWRMALRYMFRHPIRTATSLLACLLGASVM
                     ALIFCMIVGVNHLMDFHFRWLYRSDVTLFLKSERGSDALMEVAHLPGVRKVEPLLGMP
                     VTLRHKGKEKLISVTGIQGRPH"
     gene            2314..3042
                     /locus_tag="PKI05_05710"
     CDS             2314..3042
                     /locus_tag="PKI05_05710"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA51733.1"
                     /translation="MSRQLAKMMGVKPGEYIDWQPMRGDRRWYRTQVVNLMDDYVGTA
                     VYADLTFLRKCLGEGSALNSLQLAIDAGHEQRRAFVKKIRDWPEIEAMIYRADMENNL
                     RKTLLQNIWVILSIEISFAGALFFGAILNGAIISLEERRRELATFYVMGYTPWQIGGL
                     LGRESVVVVIGGLILGIPVGYFFARLVVASYQSEVLRMPLLFPPQVWIQTAIWGCLFS
                     LVSHFVVQIMIHRSRWAEAVKVAE"
     gene            3255..>4117
                     /locus_tag="PKI05_05715"
     CDS             3255..>4117
                     /locus_tag="PKI05_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOA51734.1"
                     /translation="METTPCRRGNIQEFVSEKGVTRLRDTVLLTMPFSGRIEAIPWIE
                     GTEVKKGQVLARIVPSDVEQMLEEARAVVARIEAAIRTQKDNRVEETAIAQAQAYVES
                     MKSTVAAAAARVEASEARYNYAQKYAARIAGLVQSGGASQDDLDRTELLKIESALSYQ
                     QDRLVHAALVSLQAATNLLPVLIQKYVERKALVEDELVQQLAEAKARLAMVEENARRA
                     TMVSPYDGIVLHRYVSQERFLAAGTQLLELGRWHDLEIETEILTQDAVRIKQGMLVDV
                     FLGEDLARPVCR"
BASE COUNT          891 a         1028 c         1219 g          979 t
ORIGIN      
        1 atgtttatca tagggaattc cttgtcgttt tggggccaag cggctcaggc aaatccaccc
       61 tgttaaatct tttgggcgga ctcgacacgc ctacagaagg gcaggtcctg tttgaaggac
      121 gcgacctggc agaatttgat cggcgagaac tgacgtggta tcggcgggaa atcgttggtt
      181 ttgtgttcca attctacaac ctggttgcca atcttacggc actggaaaac atcatgctcg
      241 ctgccgacct tgtcagtgat cctctggatc ccgtggatct tctagagcag gtagggctag
      301 ccgaccgggc ggatagcttt cccgcccaac tttccggtgg cgagcaacag cgggttgcca
      361 ttgcccgagc gatggtcaaa aatccgccgc taatcctttg tgatgaacca acgggcgcgt
      421 tagatgatat gactggacgt caaattctga ggttgctact caacttgaaa gaggatatgg
      481 ggaaaacggt ggtagtgatc acgcataaca cggcgattgc gcagatggcg gatcgtgtga
      541 tccgactacg cagtgggcga attgtagagg tcacggaaca agcgtcgcca ctgccgcccg
      601 aaaagattca gtggtaacgg tgaccatggt tctcaacagg acgtgacgac gcggagacaa
      661 agagatgaat cccctgcatc gaaaactcat ccgcgagtta cgagcttcag ggggtgtcat
      721 attctctgtc atcttgatca cggcgattgg gacggcctgt ttcgtggcga tgagatcggc
      781 ccaccgaagt ttgagccagg cgcgtgatca ctactatgcc aaagggcgga tggccgactt
      841 ttgggtctcg gtaaagagaa tgccgaaatc ggagttgaga cgtctttcca acattccagg
      901 ggttgcggaa gttcatccgc ggattcgttc acccgtcact gtcgatttgc cgacctcaga
      961 gaagcctctc gcaggggtgg tgttgagtct ccccgacagg ggtttgccgg cgctcaatca
     1021 aatccagatc ctgcggggag aatacttcac ttcccgacgg cgaaatgagg tcattgtgaa
     1081 cgatgtgttc gccaatgccc atcggctccg cctcggtcaa tctatctttg tcaacattgc
     1141 aggacggcga caagagctgg tgctcgtcgg tacggcgttt agctgcgagt ttatttaccc
     1201 actcggtcga gcggccattg ttccggaccg cacaggcttc ggtgtcttct ttctcaagga
     1261 gagtttcgcc gaggaaatca ctgacatgaa gggggcaaca aacgaggtgt taggacgggt
     1321 attccctcgg tatgccaact ccaagagggc tctccttcac gaaatcgaac ggcgactcga
     1381 cgaatttggg gtcctcgcca cgacacctct agaggattac ccctccaatg catttttaag
     1441 ccaggaaatc gaaggcttag caacgtttgc cttgctcatc ccgttgatct ttctagggat
     1501 ttcggccgtg attctgaatg ttgtcatgac tcggcgtgtc gagcaacagc gcatcgccat
     1561 cggaacactc aaagctctag gttatgggga cgcagtcatc ggctggcaca ttatggagtt
     1621 tgctttgctc gcgggcgtga taggggcagc cattgggtgt ggcgtcggcc agtggttgtc
     1681 ggccgggatg acaagtatct accgccagtt ctacgagttt cctcggttgg atgcgcctat
     1741 ttattgggat atccatttag aggcgatcct cgtcgggctc atttgcgccg ctatcggggg
     1801 ttggcgggga gcgagaacgg cccttgcttt gcctccggca gcggctatgc gaccacgggt
     1861 accagcttca gggcggcata tcctcctgga gcggttcggg tggctgtggc ggcatgttca
     1921 tcccgcctgg cggatggcgc tccggtacat gttccgccac cccatccgaa cggcaacgtc
     1981 actacttgct tgtttgctgg gtgcaagcgt tatggcactt attttttgca tgattgtggg
     2041 ggtcaaccac ttgatggact ttcatttccg gtggttgtat cgaagtgatg tgacgctgtt
     2101 tctcaagagc gaacgcggta gtgacgcttt gatggaagtg gcccatttgc cgggcgtgag
     2161 aaaggtagag ccgctcttgg gcatgcccgt gacattaagg cacaaaggca aggagaagtt
     2221 gatttccgtg accggcattc agggacggcc acactgacct tgccccgaga tcgccagggg
     2281 catcatatta ctatcccgcc cacggggttc ctcatgagcc gtcaactggc caagatgatg
     2341 ggggttaaac ccggcgaata catcgattgg caaccgatgc gcggtgatcg gaggtggtat
     2401 cgaacacagg tggtcaatct gatggatgac tacgtcggta ccgccgtcta tgccgatttg
     2461 acttttttga gaaaatgcct tggcgaaggg tccgccctta acagcctcca gttggcaatt
     2521 gatgccggtc acgaacaacg aagggcattc gtcaagaaaa tccgggattg gcccgaaatc
     2581 gaggccatga tctaccgcgc cgatatggag aacaacctac gaaagaccct ccttcagaac
     2641 atttgggtca tcctttcaat tgaaatctcc tttgccgggg cccttttttt tggagccatt
     2701 ttgaacggtg ccatcatttc tttggaggag cggcgtcgcg agcttgcaac gttttatgtg
     2761 atgggctaca cgccttggca gattggcggt ttgcttgggc gggaatccgt tgttgtggtc
     2821 atcggcgggc tgatcttggg gataccggtt ggctatttct ttgccaggct ggttgtcgct
     2881 agttaccaaa gtgaggtatt gaggatgcct ctcttgtttc cgcctcaagt atggatccaa
     2941 acagccatct gggggtgtct gtttagcctg gtttcccatt tcgtcgtcca gatcatgatc
     3001 caccgcagcc gatgggctga ggcggtcaag gtcgccgaat aggcatgatt ttcgcggaaa
     3061 ggcgtgttcg gagtactagc gtcatgctcg aaaaactagt taaggggttc tgaagaatgt
     3121 ccaccataaa ccgcgggctg ccttccaaaa gcagaaacaa gaagttcaag aattgggttg
     3181 gacttggtgt tgcaagtgtg ctcgtgcttg ggggcatagc atttattttg tcctctcgga
     3241 cttcctcccg agagatggag acaacacctt gtcggcgggg aaacattcaa gagtttgtca
     3301 gcgagaaagg agtgacacga ctgcgggaca ccgtgctgct gacgatgccc ttttccggtc
     3361 gcatcgaggc cattccatgg atcgagggaa cggaggtgaa aaaagggcaa gtgttggcaa
     3421 ggatcgtccc cagcgatgtt gagcaaatgt tggaagaagc cagggccgtg gtggcgcgga
     3481 ttgaggctgc cattcgaacg caaaaggaca atcgggtgga ggagacggcc attgctcagg
     3541 cccaggctta tgtggaaagc atgaagtcaa ctgtcgcggc tgccgcagcc cgggttgaag
     3601 ccagcgaagc tcgttacaac tacgcccaaa agtacgcggc acgtattgct ggtctcgttc
     3661 agtctggcgg tgcttcgcag gacgaccttg atcgcacgga actactgaag atcgaaagtg
     3721 ccctttctta ccaacaggac cgcttggttc acgccgccct ggtttctctc caggccgcca
     3781 caaatctgtt gcctgtactt atccagaagt atgtggagcg gaaagccctc gttgaagatg
     3841 aattggtcca acaactggca gaggccaagg ccaggctggc gatggttgaa gagaacgctc
     3901 gtcgtgccac catggtcagc ccctacgatg gtattgtcct acatcgctac gtgagccagg
     3961 agcgatttct cgctgcggga acgcaactgt tggagttggg acgctggcac gaccttgaga
     4021 tcgaaacaga aatcctcacc caagatgccg tgcgcatcaa acaaggcatg ttggtggatg
     4081 tattccttgg agaagacctt gccaggcccg tgtgtcg
//
LOCUS       DAOPTR010000234         4110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_17508_length_4110_cov_3.138841, whole genome shotgun sequence.
ACCESSION   DAOPTR010000234 DAOPTR010000000
VERSION     DAOPTR010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17508_length_4110_cov_3.138841"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PKI05_05720"
     CDS             <1..220
                     /locus_tag="PKI05_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51735.1"
                     /translation="FSGGCGVFDAPEPCGPWTTVFHTDRWDVGPGESGCFPTKWMSTD
                     GREMWLVFSGEDCFSLRRAKLVLRSADN"
     gene            386..1603
                     /locus_tag="PKI05_05725"
     CDS             386..1603
                     /locus_tag="PKI05_05725"
                     /inference="COORDINATES: protein motif:HMM:NF025182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HOA51736.1"
                     /translation="MKWWTVKVGVLLTGLSATISNGEPGPGTIRSPYPPSPVFAGIEL
                     DWSTHRREAQGSDLFQLCWADNDHQYGFWGDGGGFGGTNQLGRVSLGFARIEGSWNDY
                     RGFNVWGGKDAENPATFKGKSWGTVCIDGVFYSWIVPDEPDSGGPRDHYRYVELARST
                     DHGATWQKAPWRWYREDELIGPTFLVDGKNHKAAPDSYVYVYFIRPERKDITQPVVGL
                     RVQKPGAIFLARVSRQAIFQGREAYEWFTGIKGNQPVWGHLDQKRPVFENPNGVGWCL
                     SAIYHSGFQRVLLATEHTESSRGCIGLFDAPDPWGPWTTVEYWTADRPFGDQRPGSSW
                     EWKSNIFLISFVPKWFSPDGREFTLVFTGAGNGRDNDSLNTVRGRFRVNANPLKSLSN
                     NLASPIRRERGQG"
     gene            complement(1737..3566)
                     /locus_tag="PKI05_05730"
     CDS             complement(1737..3566)
                     /locus_tag="PKI05_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA51737.1"
                     /translation="MALRFYHLQQSIVMGLLAIALLALVCARADARESTRRPNVVIFY
                     TDDHGYADLGIHGVVSDIRTPHLDSLARSGVVFQNGYTTAPQCVPSRAGLLTGRYQGR
                     FGVESNKSSLEGFNKQPTIATRLQALGYVTAQFGKWHLGPANEIPQHGFQYVFSQMGQ
                     QPFYANITLDGEDRPMGIMNPEMYHIDACTKAAVSVIKRFREQPFFLYIAYRAPHTPL
                     DAPRQYTDRFPGNMPERRRQALAMISAVDEGVGQILKTLEELHLRDKTIIFFISDNGA
                     PLKISKTDSPLNTDAGGWDGSLNDPYNGEKGMLTEGGIHVPFLMSWPEKIPGGQVYQH
                     PVSTLDVAATVLAQIGADTKVGESDGVDLVPYLRGEKEGPPHESLMWRWIAQAAIREG
                     DWKLLRGGDREYLFNLKTDPSEKRNLLKDAPEVADRLRRKLVAWSGELEPPGLSTEPM
                     ARVWNAYFDFYLEGRPAPQLGANASSKAEWIARNAQIVIENGYLRLECDNPALQKAFL
                     ACAGLSLQGPVTVSLTVRADQAGQVVLAWRKAGDQDFLPGNRVVTRWEKTEGDAMVQA
                     TLPVNAQVIHIRIHPNPVQTFAIRRVVLQAANGRTETWDFGEP"
BASE COUNT          890 a         1083 c         1168 g          969 t
ORIGIN      
        1 tttttcaggg ggatgtggcg tttttgacgc cccggaaccg tgcggacctt ggaccaccgt
       61 ctttcataca gatcggtggg acgttgggcc gggtgaatca gggtgtttcc cgactaagtg
      121 gatgagcacc gatggccgtg agatgtggtt ggttttctcg ggagaagact gtttctccct
      181 gcgtcgggcc aaactcgtat tgcgatctgc cgacaactag aggtctatga gggcagtaag
      241 gaatcgatca tcgatgcatg acagattaga tttgtctcta gaagggcttt tcaagtagct
      301 gaacaggagt cgtaagaact ggccctaaac ggaatactgg cgagctgtgt gtcgggcgag
      361 gcgaaacaac agagcgatga attccatgaa gtggtggacc gtcaaggttg gtgtgttgct
      421 aacgggattg tcagccacca tatcaaatgg tgaacccggg ccggggacaa tacgttcccc
      481 atatccacca agtcccgttt ttgctgggat agagcttgac tggtccaccc atcgccgcga
      541 ggcgcagggg agtgacctgt ttcaactgtg ctgggcggac aacgatcacc aatacggttt
      601 ctggggggac gggggtgggt ttggggggac gaatcagctc ggtcgggtca gccttggctt
      661 tgcccggatc gaaggttcat ggaacgatta tcgggggttc aatgtgtggg gtggaaaaga
      721 tgccgaaaac cccgccacgt ttaagggaaa gagttgggga acagtctgca tcgatggcgt
      781 cttttactcg tggattgtgc ccgacgaacc tgacagcggg gggcctcgag atcactatcg
      841 gtatgttgaa ttggctcgct caaccgatca tggggcgacg tggcagaaag cgccgtggcg
      901 atggtaccgc gaggatgaac ttatcggacc cacgttcctc gtcgatggta aaaaccacaa
      961 ggctgcgccg gactcttacg tgtatgtcta tttcatccgc ccagaaagga aggatatcac
     1021 tcagcctgtg gtggggttgc gggtgcagaa gccaggagcg atctttcttg cccgcgtttc
     1081 ccgccaggcc atctttcaag ggcgcgaagc ctacgaatgg tttacgggta taaaaggcaa
     1141 ccaaccagtt tggggacact tggaccagaa gcgacctgta tttgaaaatc ccaatggtgt
     1201 tgggtggtgc ctaagtgcca tttatcattc aggttttcag cgagtgcttc tcgctacgga
     1261 gcacaccgaa tcatctcgtg gctgcattgg gctttttgac gcaccggatc cctgggggcc
     1321 gtggacaacg gttgaatact ggaccgcgga caggccgttt ggtgaccagc ggcccggcag
     1381 ttcctgggaa tggaagagca atatctttct catctccttc gtcccaaagt ggtttagtcc
     1441 cgatggacgg gagtttactt tggtttttac cggcgccggc aatggacgcg ataatgactc
     1501 gctcaatacg gttcgagggc gttttcgtgt gaatgccaat ccgcttaaga gtctatccaa
     1561 caaccttgcc tctcccattc ggcgggagag gggccagggg tgagggtggc tcgaatcgct
     1621 gcccgttttc tcgatcgact ccgacgatgg cttaccctca ccccaaccct ctcccaaagg
     1681 gagagggaac tttgcgatac gtctttagtg ggttgccccc aatcctgacc actcgttcaa
     1741 ggctctccga agtcccatgt ctcagttcga ccattggcgg cctgcagtac aacgcgacga
     1801 atcgcaaatg tctggaccgg attgggatga atgcgaatat ggatgacttg tgcgttcaca
     1861 ggaagagtcg cttggaccat cgcatcaccc tctgtttttt cccaccgggt aacgacgcgg
     1921 ttgcccggca aaaagtcttg atcgcccgcc tttcgccatg ccagaacaac ttggccagct
     1981 tgatcggccc gaacagtcag actcaccgtt acaggcccct gaagtgaaag gcccgcacag
     2041 gcgagaaagg ctttctgcaa agccgggtta tcgcattcga gacgtaaata accgttttca
     2101 ataacgattt gcgcattccg agcaatccac tcagccttgg aagaagcgtt agctccaagt
     2161 tgaggagctg gtctgccttc caaataaaag tcgaagtaag cgttccaaac ccgggccatc
     2221 ggttcggtac ttaacccggg gggctctagt tccccggacc aggcgactaa tttccgacgc
     2281 aaccgatcag cgacctcggg ggcatctttg agcaagtttc gcttttcgct gggatcggtc
     2341 ttcaagttga aaaggtactc ccgatctcca ccacggagga gtttccagtc accttcgcgg
     2401 atggcggcct gggcaatcca tcgccacatc agcgactcat ggggtggtcc ctccttctcg
     2461 ccccgcaaat acggaacgag gtccacgccg tctgactctc ccacttttgt gtccgcgccg
     2521 atctgagcta acacggtggc tgccacatcc agagtgctga ccggatgctg atagacttga
     2581 ccgcctggga ttttctccgg ccaactcatc aaaaagggaa catgaattcc gccctcggtg
     2641 agcatccctt tctcaccgtt atagggatcg ttaagactac catcccatcc cccggcgtcg
     2701 gtattcaggg gcgagtcggt cttgctgatc ttaagcggcg caccattatc actgatgaaa
     2761 aagatgatgg tcttgtccct taagtgtagc tcctcgaggg tcttgagaat ttggccaacg
     2821 ccctcatcca cggcagaaat catggccagg gcctggcgac ggcgttccgg catgttaccg
     2881 ggaaaacggt ccgtgtactg tcggggggca tcgagcggcg tgtgcggtgc gcggtaggcg
     2941 atgtaaagaa agaaaggttg ttcgcgaaac cgcttgataa cagaaacggc ggccttggtg
     3001 caggcatcaa tgtgatacat ctcgggattc ataataccca ttgggcgatc ttcaccatcc
     3061 agcgttatgt tggcatagaa cggttgctgg cccatctggg aaaacacgta ttgaaagccg
     3121 tgttggggga tctcgttagc cggtcccagg tgccattttc caaactgggc cgtcacatag
     3181 cccagtgctt gtaaccgcgt cgcgatggtg ggctgcttgt tgaagccttc taaggacgac
     3241 ttgttgctct caacgccgaa tcgcccttga tatcttcctg tcaaaagccc cgcccgcgag
     3301 ggcacgcatt ggggggccgt tgtgtagccg ttctggaaga caactccgct tcttgccagg
     3361 ctgtcgagat gcggcgtcct gatatcggac acaacaccat ggatccccag gtctgcgtag
     3421 ccatgatcgt cggtataaaa gatcaccaca ttcggtcttc gggtactttc ccgggcatcc
     3481 gctcttgcgc aaacaagggc gagcaatgcg atcgctaaaa ggcccatcac tattgactgt
     3541 tgcaaatgat aaaagcgtaa ggccatgaat tacctccccg atgtaattgt gtcatgctat
     3601 gatgctgctg tgctcatctt cttttttaga ttcatttagc acgatcgacg attgctaaga
     3661 atgtgaaagc tatcgttggt cccggtgtga gagaacacga ccctacacct ttcccggact
     3721 cgccaagaag cgacgacggg tcggtgttta ggcaggccgt caaatcgctc caaagaaacc
     3781 tgatgatcca accctttagg agcctatctt ataatctcct ctcgcccgtt cggcgggaga
     3841 ggggtcgggg gtgagggtgg gtcgaatcgt tgccggtttt ctcaattgat tctgaccttg
     3901 gcttgccctc accccaaccc tctcccggag ggagaggggg aacgttttgg gatagactaa
     3961 gatttccaag caccggtcga tctgtactgt aattcgcgag cgtgttaacc tgccgaccca
     4021 cagcgccggc aagctctggt ccattatgtt acactctcaa aaccgtttgt ctataatggg
     4081 ctcagccatt tcgattccct tgggggcgac
//
LOCUS       DAOPTR010000235         4109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_17512_length_4109_cov_3.216083, whole genome shotgun sequence.
ACCESSION   DAOPTR010000235 DAOPTR010000000
VERSION     DAOPTR010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17512_length_4109_cov_3.216083"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PKI05_05735"
     CDS             <1..625
                     /locus_tag="PKI05_05735"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOA51738.1"
                     /translation="ENGVKYLVFEGSPYYTMHKACDVWEYGLMEDEKVGTRALELLEK
                     YKDRPFFFFVHFASVDHQGHRFGENSKEYNDALISNDTWTGKIIAKLKELGLYDKTLV
                     YVTADHGFNEGETRHSYAPYVILATNDPLVKRDGMRQDIAPTILARFGLDLSRFQPPL
                     DGEPLTQPATKPVLKAPETPPARPKAQSRQPGQRKLQKQAAPVSQAP"
     gene            complement(737..2110)
                     /locus_tag="PKI05_05740"
     CDS             complement(737..2110)
                     /locus_tag="PKI05_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOA51739.1"
                     /translation="MSPKTSDRLPTILIVDDERSHRALFRDLITNLGFAVEEASQAEE
                     ALEIVSRHPPQLILLDVRLPGQSGLELLPVLKEKVPTVPIILITAYGDVREAVAAMKI
                     GAVDYLLKPVDLEELEAIILDYLGCETASAPRPAVSLPPLGDVVCVSLGFRRVLETAY
                     LAARSDIPILILGPSGSGKEVVARLIQQWSHRKDGPFIVANCAGLPESIVESELFGHT
                     KGAFTGAQTERLGYFRAADGGTLFLDEIGDLPPPVQAKLLRALETGEIVPVGSERPIR
                     VDFRLLAATNRDLTTLVREGRFREDLLFRINAVELVVPPLKDRVEDILPLAEFFAKRF
                     TKRVVRFSPQAEACLLAYSWPGNVRELRNAVQRACLLSQGEIILPEHLPPSVALTSGI
                     TSDRQSTADEGRLSAMERATILATLAECRGNRTQAAKKLGISRRALIYKIRAIESQLN
                     HSSRDKD"
     gene            complement(2131..3504)
                     /locus_tag="PKI05_05745"
     CDS             complement(2131..3504)
                     /locus_tag="PKI05_05745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOA51740.1"
                     /translation="MPKISQVIAVFILIAGAVMFSLWQWYEYQADVAAAREVLHGHAE
                     GLCRAMVGAIRSHRRLGAFFDQQIQIILDELVDSPDVLAVQIADSAKTPIFRSGSSDV
                     LTLSVGGSFADVGYVYSASFQLAPLAAAGPQGGARGFGWGRWARGVAPEPDTSPFASG
                     GLFYATVVLDRKGFDNHVRRIARSRAGLCLTGWIALLLFGLTFRSSVRMVKADHAKRL
                     AELQARHFEELSQAASGLAHETRNPLGLIRGRAQRIAHREDPTSPTATDARLIVEECD
                     RLTARLTQFLLFAKPFTLQPVEVDADVVIEEVLLLLEPDCQSKDVRCVHHKSPQSVPI
                     VADRELTRQALFNLLHNAVKYTRPHSEITVISRVDGSNVIIEIIDQGPGVSPEHIPKL
                     FMPYFSTDSAGTGLGLSLVRKIAVAHGWDISYCPSPEGGAMFRLTIPLKTTGSGTHIG
                     EFGHTRR"
     gene            complement(3685..>4109)
                     /locus_tag="PKI05_05750"
     CDS             complement(3685..>4109)
                     /locus_tag="PKI05_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51741.1"
                     /translation="VTILLGMTAPVWAQNQEDKSQTPSVTSSGSGVLSPAQQRPEWFK
                     TMAAIAEEQAKPQPDQQKIAALQQKLFQLRQQGFAPGWQPGIGFQAPPTMMRPCPWGG
                     PGLGMGMGRGPGPGGPRGPGYGMGAPMGRGYRGGPRMW"
BASE COUNT          938 a         1167 c         1150 g          854 t
ORIGIN      
        1 agaaaatggg gtcaaatacc ttgttttcga ggggtcgccc tattacacga tgcacaaggc
       61 gtgtgacgtt tgggagtatg ggctgatgga agatgagaag gtcgggacgc gggccctgga
      121 acttctcgag aagtataagg atcgaccgtt tttcttcttc gtccacttcg cctcggtcga
      181 tcaccagggg caccggtttg gggaaaactc aaaagagtac aacgatgcct tgatctcgaa
      241 cgatacgtgg acagggaaaa tcattgccaa acttaaagag ttgggcttgt acgacaagac
      301 gctcgtgtat gtgacggcag accatgggtt taatgagggt gagacccgtc attcttatgc
      361 cccctatgtt atcctggcga ccaacgatcc tttggttaag cgggatggga tgcgtcaaga
      421 tatagcgcca accattctcg cgcggtttgg cctcgactta agccgtttcc aacccccatt
      481 agatggtgaa cccctgacgc agcccgccac aaagcccgtc ctgaaagccc cggagacgcc
      541 gcctgcccgt cctaaagcgc aatcgcgaca gcctggccaa cggaagctcc agaagcaagc
      601 ggccccggtt tcgcaagccc cgtaatcggg gacgtgggtt gggcgaacta gggagcctac
      661 tacgaacact ggcaggttga agatgccatg cgaagggtgt gtggacttgg acacgccatt
      721 tgtggcggga cgtaccttag tctttgtccc tagacgaatg attcagttgg gattcaattg
      781 cccgaatctt gtaaatcaag gcacggcggc tgataccgag cttcttggcg gcctgggtgc
      841 gatttccgcg gcattcggcc aatgtcgcca ggatagtcgc ccgctccatg gccgaaaggc
      901 ggccttcgtc ggcggtggat tgccggtcag aggtaatgcc ggaggttaaa gccaccgacg
      961 gcggaagatg ttcgggcaaa atgatttccc cttgggacag gaggcaggcc cgctgaaccg
     1021 cgttacgcag ctcccgcaca ttgcctggcc aagagtatgc caaaaggcat gcctcggctt
     1081 ggggcgagaa acgaacaacg cgtttggtga aacgtttcgc gaaaaattct gccagcggga
     1141 ggatgtcttc cacccgatcc ttcagcgggg gcacgacaag ctcgacggcg ttgatccgaa
     1201 aaagcaaatc ctcccgaaat cgtccctctc gcacgagggt cgtcagatcg cggttcgtgg
     1261 cggccaacaa gcggaaatct acccggatcg gacgctcgct tcccaccggt acaatctcac
     1321 cggtctccag cgcccgcaag agcttggctt ggacgggcgg cggtaggtcg ccaatttcat
     1381 ccaaaaagag ggtaccaccg tctgccgcgc gaaaatagcc cagcctctcc gtctgggccc
     1441 ctgtgaacgc ccctttggtg tgcccaaata gttcgctttc gacaatcgac tccggcaatc
     1501 ctgcacagtt agccacaata aaaggcccat ctttgcggtg actccactgc tggatcagtc
     1561 gagcgacgac ttctttgccg ctcccacttg ggcccaaaat aaggatcgga atgtccgaac
     1621 gggctgccag gtatgccgtt tccaagacac ggcgaaatcc caagctgacg cacacaacat
     1681 cgcctaatgg gggcaaggag acggccggac gcggtgcgga agcggtctca cagcctagat
     1741 aatcgagaat gatagcctcc agttcctcca ggtccaccgg ctttaggagg tagtcaacgg
     1801 ccccgatttt catcgccgcc accgcctctc ggacatcacc gtaagcagtg atgaggatga
     1861 tgggcactgt ggggaccttc tctttgagaa cgggcaagag ttctaggccg gattggcctg
     1921 gcagacgcac atcgaggaga ataagctgcg gcgggtgccg agaaacaatt tccaaggctt
     1981 cttctgcctg cgaggcctcc tcgaccgcaa aacctaagtt ggtgatgaga tcacgaaata
     2041 gcgcacgatg ggacctttcg tcgtcaacaa ttaaaatcgt aggtaaacga tcggacgtct
     2101 tagggctcat atcgggctaa caatccttac ttatcgcctg gtgtggccaa attccccgat
     2161 gtgcgtgccg ctgcctgtgg tcttgagggg tattgtcaga cgaaacatgg ccccgccttc
     2221 cggggacgga caatagctga tgtcccaccc atgggccaca gcaatctttc gcaccagcga
     2281 aagcccaagt cccgtgccag cagaatctgt actgaagtag ggcataaaca atttcggaat
     2341 atgttcggga ctaacacctg gcccctggtc gatgatctcg attataacat ttgagccatc
     2401 gactcggctg ataactgtga tctcggaatg aggacgagtg tacttgacgg cgttgtggag
     2461 caggttaaag agtgcctgtc gcgtcaactc gcggtctgcc acgatgggaa ccgactgcgg
     2521 cgatttgtga tgcacacacc gaacatcctt actctggcaa tccggctcca aaagaagtag
     2581 cacctcctcg attaccacgt ccgcatcgac ttcgacaggt tgaagagtga acggtttggc
     2641 aaaaagaagg aactgtgtca agcgggcggt caaacggtcg cattcctcga cgataagccg
     2701 ggcatcggtc gctgttgggg atgtcgggtc ttcgcgatgc gcaatccgtt gggctcgtcc
     2761 ccgaattagt cccaggggat tgcgggtttc gtgagccaag ccactcgctg cctgacttag
     2821 ctcctcgaag tggcgagcct gtaactcggc cagcctcttg gcatgatctg ccttgaccat
     2881 gcggacactt gagcggaagg tcaaaccaaa aagaagtaat gctatccagc cagtcaaaca
     2941 taacccagcc cgtgatctgg caattcggcg gacatggttg tcaaagcctt ttcgatcaag
     3001 caccacagta gcgtagaaca atcccccgga cgcaaacggg cttgtgtcgg gttccggtgc
     3061 aacgccgcga gcccaccgac cccacccaaa tccacgggcc cctccctggg gaccagcggc
     3121 cgccaacggc gccagttgaa agcttgccga gtaaacgtat cccacatcgg cgaaactgcc
     3181 cccaacactc agggtgagga cgtcgctcga cccgctacgg aagatgggcg ttttcgcact
     3241 atcggcgatc tgaacggcca aaacatcggg cgagtcaacc agttcgtcga ggataatttg
     3301 gatttgttga tcgaaaaacg cgcccaatcg gcggtgggaa cgaattgccc ccaccatcgc
     3361 ccggcaaagc ccctcggcgt ggccgtggag gacttctctt gccgcagcca cgtccgcttg
     3421 atactcatac cattgccata gggaaaacat gactgcgccg gcaataagta tgaaaacagc
     3481 gataacttgg gagatttttg gcatgcgtca ggcctcaccg gcgtttgttc atgctgcaac
     3541 gtacaaaatg gattcgagct tttctgaaaa agaaaaagcg gtcgagccgg aagctccacc
     3601 gcttttttgg cctatcttca ggatgttgtc aactcggaat ctcgcacctg tgcaacgata
     3661 acgtcgcagt tcgatgcatg gtgattacca catccgtggt cccccgcggt aaccgcgtcc
     3721 catcggagca cccattccat agccggggcc tcttggtcca ccagggcctg gtccacggcc
     3781 catccccatt ccgaggcccg gtccacccca cgggcaaggc cgcatcatcg tgggtggtgc
     3841 ctgaaaacca attcccggtt gccatcctgg tgcgaaacct tgttgccgca actgaaagag
     3901 tttctgctga agggcggcaa tcttttgttg gtccggctgt ggcttggctt gctcctcagc
     3961 aatcgcagcc atagtcttga accactcggg ccgttgctga gcaggggata gcaccccgga
     4021 cccacttgat gtcaccgatg gggtttgaga tttatcttcc tgattttgtg cccaaacagg
     4081 tgccgtcata cccaaaagaa ttgttacgc
//
LOCUS       DAOPTR010000236         4105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_17529_length_4105_cov_3.661235, whole genome shotgun sequence.
ACCESSION   DAOPTR010000236 DAOPTR010000000
VERSION     DAOPTR010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17529_length_4105_cov_3.661235"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PKI05_05755"
     CDS             <1..249
                     /locus_tag="PKI05_05755"
                     /inference="COORDINATES: protein motif:HMM:NF022694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HOA51742.1"
                     /translation="GEGDLVHHSYRVLLFGRIEGYVQILRRGPAQDWVTETRNVVEDH
                     KQFFRSSQPRVVAIGIWCDSNDTASVSEGMIGPLVFSK"
     gene            435..3191
                     /gene="polA"
                     /locus_tag="PKI05_05760"
     CDS             435..3191
                     /gene="polA"
                     /locus_tag="PKI05_05760"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOA51743.1"
                     /translation="MSDDQGQRDKPPVNGGPSLDGQTVIVVDAHSLIHQLFHAISELT
                     SPDGRPVNAVYGFVRDMIHLLKTYQPSYLICAFDAKEPSFRKELYADYKAHRPELDAD
                     LVPQIELIEEFLHALGIPVLRQPGFEADDIMATIANEVERQGGECLLVTTDKDCRQLI
                     SDRVKILNLRKNAILDHAGLLEEWGIQPEQVVDFQALVGDSSDNVPGVPKIGPKTARD
                     LLAKYGNLESILAHLDEMPKKTWSESLRAHIDQALLSRQLVTLRRDVPLDLNWQNLRL
                     RPANRALVFDLCRRLGFGKLLSELQELLPKEELTIKKAQIEYRTIVVESLEQLRQLVA
                     QWTNVSEISLDTETTDIRPRWADLVGISLAVSLEEGYYIPIRSPLGEVRLPEAQVLDI
                     LRPILTNAQIKKIGQNLKYDLVVLRSAGVKVEGVGFDCMLADYLLSPGSRSHGLDELA
                     QRVLGHQTIKIDSLIGKGKRQLTMDQVSISRIAEYAVEDAILPWHLKQRLVPELERNS
                     LLTLFSELEVPLVEILAEMEYLGVRVDERRLSDLGREFESRMADLEREIYELAGRTFN
                     ISSPKQLQEVLFEQLKLPSSKRTKTGISTDSEVLEELAEEHPLPAKIIEYRQLAKLKS
                     TYIDGLREMVCPRTGRVHASFHQAVTATGRLSCSDPNLQNVPVRTEEGRAIRSAFVAP
                     EAGWVLLSADYSQIELRVLAHFSQDSGLCEAFQRDEDIHTAVAAQVFGVSMDQVTPEM
                     RRRAKTVNFGVIYGQTPFGLAKQLHITKEEAAQFIEAYFNRYPGIDRFLAQVLEDCRR
                     TGYVSTILGRKRFVTGIRPGFTRQRNLPERTAINTVIQGSAADIIKKAMLRVYKRLHE
                     ESSPARMILQVHDELVFECPEEALSRTIAIVKEEMENVMTLRVPLKVDVAWGKNWADL
                     QSVA"
     gene            3272..3883
                     /gene="coaE"
                     /locus_tag="PKI05_05765"
     CDS             3272..3883
                     /gene="coaE"
                     /locus_tag="PKI05_05765"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOA51744.1"
                     /translation="MWILGLLGGICSGKTFVAQEFARYGAQVFDADRSVHELLNREDI
                     CHRIAQCFGPGALDADGRVNRGYLASLVFGSDAESARHRKWLEELLHPEVYASLQEAM
                     VKARQLGCALFVIDAPLLLEANWSTLCHRLVYIDAPDDLRYARATERGFDRAELARRE
                     TAQASLNLKKVRADYVIHNSLNWQQTQNQVARIVEWLARDVIA"
     gene            complement(3926..>4105)
                     /locus_tag="PKI05_05770"
     CDS             complement(3926..>4105)
                     /locus_tag="PKI05_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51745.1"
                     /translation="TPCGHSHAIGFINSPGWTQPEALIIIFDFVPVFSRVEKPPQQPT
                     MIRQLLMAFSPKPFP"
BASE COUNT          969 a         1058 c         1141 g          937 t
ORIGIN      
        1 ggggaaggcg acctcgttca ccactcatac cgcgttctcc tttttggcag gatcgagggt
       61 tatgtccaga tcttgcggcg tggcccagcc caagattggg tcaccgaaac tcgcaacgtc
      121 gtagaagatc acaaacagtt ttttcgaagc tctcagccac gagtcgtggc catcggtatc
      181 tggtgcgatt ccaacgatac ggcgagtgtt tctgaaggca tgatcggccc actcgtgttt
      241 tccaaatagc cttaagatct agggcatctc gtccgtcgtc atccttgttt tgaagtcagt
      301 ctgttgtctt acgtcaggag gaaggtggcg agggctgtcg aaatatccca aaatgggaat
      361 tgctgcgctt ctttggacca gcccatgact cttggcgtaa tagggctcat atttggtagc
      421 gacgaggtct ttcgatgtca gacgaccagg gacaacgtga caagccgccg gtaaacggag
      481 gaccgtcatt agacggtcaa acggtgattg tggttgatgc ccattctctg atccaccaat
      541 tgtttcacgc aatcagcgaa ttaacatcgc cggatggccg ccccgtcaat gcagtttacg
      601 gctttgtccg ggatatgatc cacctcttga agacgtacca accaagttac ctcatttgcg
      661 catttgatgc caaggagcca agctttcgaa aagagcttta tgccgattac aaggcccatc
      721 gtcccgagct tgatgccgac ctagtacccc aaatcgaatt gattgaagaa ttcctccacg
      781 ccctcggaat ccccgtcctg cgtcaaccgg ggttcgaggc ggacgacatc atggccacca
      841 ttgcgaacga ggtcgagaga cagggcggcg agtgtcttct ggttaccact gacaaagatt
      901 gtcgccaatt aatcagtgac cgcgtgaaga tcctcaacct ccgaaaaaac gccatcctgg
      961 atcacgccgg cttattggag gagtggggga ttcagccgga acaggtggtt gacttccaag
     1021 ccctggtagg cgatagcagc gataacgtcc cgggtgttcc gaaaatcggt cccaagacag
     1081 ccagagacct actggcaaaa tacggcaact tggaatctat tctggcacat ctggacgaaa
     1141 tgccgaaaaa aacctggtcg gaaagcctac gtgcccacat agatcaggct ttattatctc
     1201 gacaactcgt cacattgcgg cgggatgtgc ctttggacct taattggcag aatctccgct
     1261 tacgtcccgc caatcgagcc ttggtctttg atctgtgccg ccgtcttggc tttggaaaac
     1321 tgctaagcga actgcaggaa ttgctaccca aagaagaact gacgatcaaa aaagcgcaaa
     1381 tcgaataccg aacgatcgtg gtggagtcct tggagcaact ccgtcaactc gtcgcccagt
     1441 ggactaatgt ttcggaaata agtctggata ccgagacgac cgatatccgt ccccgttggg
     1501 ccgacctggt gggcatctct ctcgccgtaa gcctcgagga gggttactac atccccattc
     1561 gttcgccatt gggcgaagtg cgtctcccgg aagctcaggt cctcgacatc ctcaggccaa
     1621 tcctgacaaa cgcacaaatc aaaaagatcg ggcagaatct caaatacgat ttggtggtgc
     1681 ttcgatcggc cggggtgaaa gttgaagggg ttggttttga ctgcatgttg gccgactacc
     1741 tcctatcgcc gggttcccgt tcgcacggcc tggacgagct tgcccagcgg gtgctcggtc
     1801 accagaccat caagatcgat tcgctcatcg gtaagggcaa gcgacagttg acgatggacc
     1861 aagtaagcat ttcccggatc gctgagtacg cggtggaaga cgcgatccta ccctggcact
     1921 tgaaacagag gctagtgccc gaattggaac gcaacagcct gctaaccctg ttctccgagt
     1981 tggaggttcc gctagtggaa atcctggcgg aaatggaata tctcggcgtc cgggtcgatg
     2041 agcgtcggct ttcggatttg ggacgcgagt ttgaatcgcg gatggccgac ttggaacggg
     2101 aaatctatga actcgccggt cgaaccttca atatctcttc cccgaagcaa ctccaagagg
     2161 ttctttttga gcagctcaaa ctaccaagca gcaaacggac gaagacaggg attagcaccg
     2221 attcagaagt tctcgaggag ttggcggagg aacatccctt gcccgccaag atcatcgaat
     2281 atcgccaatt agcaaagctg aaaagtactt atatcgacgg tcttcgagaa atggtctgcc
     2341 cccggacagg ccgggtccac gcttcgttcc atcaggccgt cactgccact ggtaggctga
     2401 gctgtagcga ccccaatctt caaaacgtcc cggttcgcac cgaggaaggg cgggccattc
     2461 gttcagcctt cgtagctccg gaggcaggtt gggtcctcct ctcggccgat tattctcaaa
     2521 ttgaattgcg agttctggcc catttttccc aagacagcgg gctatgcgag gcatttcaaa
     2581 gagatgaaga tatccacacg gccgtggctg cgcaggtgtt tggcgtatct atggatcagg
     2641 tcacgccaga gatgcgacga cgggccaaaa cggtcaactt tggggtaatc tatgggcaaa
     2701 caccctttgg actggcaaag cagttacata tcaccaagga agaagcagcc cagttcatcg
     2761 aggcctattt caaccgatat cccgggatcg atcgctttct cgcccaggtc ttggaagatt
     2821 gtcggcgcac gggttacgtg tccaccatcc tcggtaggaa acgatttgtg acagggattc
     2881 gccccggttt tacccgccaa cgcaatttgc ccgagcgaac tgccatcaac acggtgatcc
     2941 aagggtcggc agcggacatt atcaagaagg ctatgctaag ggtgtataag cgacttcatg
     3001 aggaatcgag tcccgcccgc atgattttgc aggttcacga cgaacttgtg tttgagtgtc
     3061 ccgaggaagc gctgtcacga accatagcaa ttgtcaaaga agagatggaa aacgtaatga
     3121 ctttgcgggt tcccttgaag gtagatgtcg cctggggaaa gaactgggcc gatctacaat
     3181 ctgtcgcgta gaccaacaag aaggaacttc agtaaacggc agaagccatc ccctcagtgt
     3241 aggggtatct atttcgcaaa tgggttgatc catgtggatt cttggccttt tagggggtat
     3301 ctgttcgggg aagacttttg tggcccagga gtttgcgcgt tacggggctc aggtctttga
     3361 cgccgaccgc tccgttcatg aactcctcaa cagagaggac atttgccacc ggattgccca
     3421 gtgtttcggc cctggggcgc ttgatgccga cggtcgtgtt aatcgggggt acctggcaag
     3481 ccttgttttc ggttcggatg cagaatccgc gcgccacaga aagtggctgg aggagctcct
     3541 ccaccctgag gtgtacgcca gcctccagga ggcgatggtg aaggcgcgtc aattggggtg
     3601 tgcgttgttt gttattgatg ccccattgct cttggaggcc aattggagta cactatgtca
     3661 caggctggtt tatattgacg cccccgatga tttgaggtat gctcgcgcta cggagagggg
     3721 gttcgatcgc gcggaattgg ctcgccgtga gaccgcgcaa gcatccctga acctcaaaaa
     3781 ggtccgcgcc gattatgtga tccataacag cctcaattgg caacagaccc agaatcaggt
     3841 cgcccggatt gtagaatggt tggccaggga tgttatcgcg tgatacttga gaacaaaacg
     3901 ggagcaaggt agtttcccag ccttttcatg ggaagggttt tggactaaaa gccatcaaca
     3961 gttgtcggat catcgtcggt tgctggggcg gtttctcaac ccgagagaac actgggacaa
     4021 aatcgaatat aatgattagg gcttcaggct gggtccagcc tggagaattg atgaagccaa
     4081 ttgcgtggct gtgcccacaa ggggt
//
LOCUS       DAOPTR010000237         4090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_17603_length_4090_cov_3.307807, whole genome shotgun sequence.
ACCESSION   DAOPTR010000237 DAOPTR010000000
VERSION     DAOPTR010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17603_length_4090_cov_3.307807"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            251..1114
                     /locus_tag="PKI05_05775"
     CDS             251..1114
                     /locus_tag="PKI05_05775"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOA51746.1"
                     /translation="MSRLVHGESAAKRSARVEYENGKSRSSGVPRIQPSPYFRWHAIW
                     AWILTALATPVFLPIIGFLVLLVRLTSRGPGLYRQQRVGKNGKIFWIYKIRTMRIDAE
                     AHTGPVWTEENDPRITPVGRWLRRLHLDELPQIFNVLMGQMTLIGPRPERPEFTQMLA
                     QAIPGYLNRIAVLPGITGLAQINLPPDSDLDSVRRKLVLDLEYIEKANFWLDVRILLC
                     TVSKMVGLPGLKIAGWLGLLRPARIPPWMFNGKTGSHQIPCTYSQLQRRSQDKTIHFA
                     ETRRNLFRKPR"
     gene            1125..2018
                     /locus_tag="PKI05_05780"
     CDS             1125..2018
                     /locus_tag="PKI05_05780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HOA51747.1"
                     /translation="MPLVHALTFDVEEYFQVSAFERVVSRLRWDEYPSRVGPFVEKIL
                     DILAEHGVSATFFVLGWIAKKYPKLIRLIHSMGHEIGSHSMEHRLVYDLSPGEFREDL
                     RQSLKLLEDHTGERVTVFRAPSFSITSRSLWAIEILAEEGITCDASIYPIVHDRYGIP
                     RARLEPHRIRTDSGIIWEFPGTALSWMGLRLPLGGGGYLRLYPLWLTRRLFQRVIRQQ
                     RPIMMYVHPWELDPDQPRIGGISPVSRFRHYVNLATTEKKLRALLRAFSWGSLRESLA
                     SWQSQGVDPDDVVPMSTLARG"
     gene            2160..3389
                     /locus_tag="PKI05_05785"
     CDS             2160..3389
                     /locus_tag="PKI05_05785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HOA51748.1"
                     /translation="MARARVAAMSRKRILYLVHRTPYPPNRGHRIRSFHWLDYLSQRA
                     EVDLAFLADEPLEEDHLTELRFRCQRVAWVRTSRLGQRMRGILSLLRGRSATEAAFYS
                     PRLERTVRRWLAETTYDAVIAYCSSMAIYLRRLGLSPNRCIVDLVDVDSQKWLDYAET
                     VAFPQAFLYHLEGNRLRRFERELDHLAKFLIFVTEQESQIFRAFHPTEKIRVVENGVD
                     LAYFDPAHSGEPTPAPQTPVRLVFVGAMDYYPNVDGVTWFLREIWPRVRNAGDVVFDI
                     VGAGAGKSFWRLVEATPGARLVGNVPDVRPYLRGSVSVVPLRIARGLQNKVLEAMAMG
                     CPVVASPAALEGLDVAVGEDVLSAQDVDNWVEQLTQLIHSPEMRSRLGAAARAKIEKR
                     YQWPDRLRVLDDLLGLS"
     gene            3492..>4090
                     /locus_tag="PKI05_05790"
     CDS             3492..>4090
                     /locus_tag="PKI05_05790"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOA51749.1"
                     /translation="MTSGVDRRRWALWQTLGKRFGIYDIPQDLRLSVVVPAYNEVETI
                     DTILQRILEVPIQKEVLVVEDGSTDGTSERLRRWESQSEVKVLRHSRNRGKGAALRTA
                     FAQATGQIIVIQDADLEYDPAEHLRLIQPIVDGVADVVYGSRFVSEGPHRVLYFWHYQ
                     ANRFITTLSNMFTDLNLTDVETCHKAFRSEVIKEILPTL"
BASE COUNT          813 a         1036 c         1222 g         1019 t
ORIGIN      
        1 ttctgcggca gttgtcaacc attggggtta cttttttgtg caacgcggat gcgcggttag
       61 ttggtaatca ttgtcggatg tgttgaattt gcaaaaattg ttgacattgc cggacaatcg
      121 ggttaggtta tgagacggta atagtgtgaa tccttcggat atcaaaaaga gggcagaagg
      181 gtaaatggtg cgagaaagat agctatcatc atttgattcg gcgtcgtcat ttggggtcgc
      241 ggaggcttcg atgagtcggc tggtgcatgg tgaatccgcg gctaagcgta gtgctcgcgt
      301 tgagtatgaa aatggaaaaa gtcgaagttc cggcgtaccg aggattcagc cgtcgccgta
      361 ttttcgctgg cacgccattt gggcttggat actcacggca ctggccaccc cggtgtttct
      421 gcctattatt ggtttcctcg tcctattggt tcggttgacg agtcggggac ccggtctata
      481 tcgccaacag cgggtgggca aaaacggcaa gatcttttgg atctacaaaa tacgaaccat
      541 gcggatcgat gcggaggctc atacggggcc ggtctggacg gaggagaacg atcctcgtat
      601 cacgcccgtt ggacggtggt tgcgtcgttt gcatttggat gagcttcctc agattttcaa
      661 cgtcctgatg gggcaaatga cgctcatcgg tccccgaccg gaacgcccag agtttaccca
      721 gatgctggcc caggccattc ccggttatct caatcgcatc gcggttttgc cgggtattac
      781 gggacttgct cagatcaatt tgccgccgga tagcgatctc gattcggtgc gccgcaagct
      841 ggttctcgat ctggagtaca tcgaaaaggc caatttttgg ctggatgttc gaattctgtt
      901 atgtacggtg tcgaagatgg tcggcctgcc agggttaaag atcgccggtt ggctcggctt
      961 gcttcgtccg gcgcgaatcc caccatggat gttcaacggc aagacgggga gtcaccaaat
     1021 cccgtgcacg tattcgcaat tacagcgccg ttcccaggat aagaccatcc attttgcgga
     1081 gacgcgccgc aatttgttta ggaaaccgcg gtgaggcgac gcccttgcca cttgtccatg
     1141 ccttgacgtt tgacgttgag gaatactttc aggtctcggc ctttgaaagg gttgtctctc
     1201 gcttacggtg ggacgaatat cccagtcgag ttggtccatt cgtagaaaaa atcttggata
     1261 ttttggcgga gcatggcgtt tctgcaacat tcttcgtcct gggttggatc gcgaaaaagt
     1321 accccaagtt gatccggctt atccattcca tggggcacga aattggctcc catagcatgg
     1381 aacatcgcct ggtttatgat ctgagcccag gggagtttcg cgaagatttg cggcagtcgc
     1441 tcaagctgtt agaggatcat acgggcgaac gtgtcaccgt ctttcgggcc cccagttttt
     1501 cgatcacaag tcggtccctt tgggcgatcg aaatccttgc cgaagagggc atcacctgcg
     1561 acgctagtat ttatccgatc gtccatgatc gttatggaat cccacgcgct cggctggaac
     1621 cgcaccgcat ccgtacggac tcgggaatta tttgggaatt tcccggaact gcgctgtcct
     1681 ggatgggcct ccgcctgccg ctgggcggtg ggggatattt gcggttgtat ccgctttggc
     1741 tgacacggcg gcttttccaa cgtgtgattc ggcagcaacg acccatcatg atgtacgtcc
     1801 atccttggga acttgatccc gaccaaccac gcattggtgg catctcccca gtaagtcggt
     1861 ttcggcatta tgtcaacctt gctacgacgg agaaaaaact acgggcctta ttgcgagcgt
     1921 tttcgtgggg ttctcttagg gaatcacttg cgagctggca aagccagggc gttgaccccg
     1981 acgatgttgt tccgatgagc actctggcgc ggggctaatg ttcgcatcac tcgcctttaa
     2041 gtcgtgagaa cgtctgtggc agcgccgccg gcttttcgaa ggaattgcgg atccgtcccc
     2101 accccgtatt ggtgtagtcc gtggtgttgg tgtacttcgt gtgagggatg tgtggcactg
     2161 tggcgcgggc tagagtggcg gccatgagtc gcaaacgcat cttgtatctg gtccaccgca
     2221 caccataccc gccgaaccgg ggccatcgca ttcgttcatt ccattggctc gactaccttt
     2281 cgcaacgggc ggaggtggat ttggccttcc tggcggacga acctttagaa gaagaccacc
     2341 tcacggagct tcgtttccgg tgtcaacgtg tggcctgggt tcggactagc cggttgggac
     2401 aacggatgcg agggatactc tcgctccttc gtggacgcag tgccacggag gcggctttct
     2461 actcgccccg gttggaacgc accgtccgcc gatggcttgc cgagaccacc tacgatgctg
     2521 tcattgccta ttgttctagc atggccatct acctgaggcg tctcggcttg tcgcctaatc
     2581 gttgcattgt ggatcttgtg gatgtggaca gtcaaaagtg gcttgactac gctgagacgg
     2641 tggctttccc gcaggcgttt ctctatcatc tggagggaaa ccgccttcgc cgctttgaac
     2701 gcgaactgga tcaccttgcg aagtttctca tcttcgttac tgaacaggaa agccagattt
     2761 ttcgcgcgtt tcatcccacg gagaagattc gcgtcgtcga aaatggcgtc gatttggcct
     2821 acttcgatcc cgctcatagc ggcgagccaa cgccggcgcc acaaacaccg gttcgtctcg
     2881 ttttcgtcgg ggcgatggat tattatccca acgtggatgg cgtgacttgg ttccttcgag
     2941 aaatctggcc acgggtacga aacgcgggtg acgtggtgtt tgacatcgtc ggggcggggg
     3001 cggggaagtc tttttggcgt ctggttgaag cgaccccggg ggctagactg gtgggtaatg
     3061 tgcccgacgt gcgtccgtat cttcgcggga gtgtaagcgt tgtcccgttg cggattgctc
     3121 gcgggttgca gaacaaggtt ttggaagcca tggcgatggg ctgtcctgtg gtggcctcac
     3181 ccgcagccct ggagggattg gatgtggccg ttggggagga tgtcctttca gcccaggacg
     3241 ttgacaactg ggtggagcaa ttgacacagt tgattcattc accggagatg cgaagccgcc
     3301 ttggcgcggc tgcgcgagcg aaaatcgaga agcgctacca atggcccgat cgccttcggg
     3361 tactcgatga tttgttggga ctctcgtaag gaccgaattg attgtggcga attcggtaaa
     3421 agagaaccag ggaagaaaga cctcatatcc cgtttatggc tgacgattgt tgtccccttt
     3481 gcggatctgc tgtgacatcg ggcgtggatc gccgccgttg ggctctgtgg caaacgctgg
     3541 gaaaacgttt tgggatctat gacatcccac aggatttgcg actctctgtg gtcgtaccgg
     3601 cgtacaacga agtggaaacg atcgacacaa ttctgcagcg aattctcgag gttcccatcc
     3661 agaaggaagt cctcgttgtg gaggacggga gcaccgatgg tacgagtgaa cggctccgtc
     3721 gctgggagtc gcaaagcgag gtgaaggtct tgcggcactc taggaatcgt ggcaagggcg
     3781 cggccctgcg gacggcattt gcccaagcca cgggacaaat tatcgttatt caggatgcgg
     3841 acttggaata cgatcctgcg gagcatctgc ggctgattca acccatcgtg gatggtgtgg
     3901 ccgacgtggt ttacggatca cggtttgtgt ccgaaggacc gcatcgtgtc ctctattttt
     3961 ggcactacca ggccaaccgc tttatcacca cgctctccaa tatgtttacg gatttgaatt
     4021 tgacagacgt cgaaacctgc cacaaggcct ttcgcagtga ggtcatcaag gagattctgc
     4081 ccaccctcaa
//
LOCUS       DAOPTR010000238         4088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_17612_length_4088_cov_3.346888, whole genome shotgun sequence.
ACCESSION   DAOPTR010000238 DAOPTR010000000
VERSION     DAOPTR010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17612_length_4088_cov_3.346888"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2451
                     /locus_tag="PKI05_05795"
     CDS             <1..2451
                     /locus_tag="PKI05_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOA51750.1"
                     /translation="PVSWSPEGANVTCRYHLGEGALLWDEFHPHIYALQVTLETAQTR
                     DTGDPAGLQHTRTVTFGLRDLEVRGTQFVLNGRPIFLRGTLECCIFPIHGYPPTDRES
                     WRKVFATARDYGLNHLRFHSWCPPQAAFEVADEMGFYLQVECSCWASFGDGGSQDAFV
                     YEEAERIRRAYGNHPSFLLMVASNEPGGRNSNQFLAKWVETQIAADPRRCYSAGSGWP
                     QLAVNQFHVQPRTRLQNWGPLQLNKPPQTWDDYREYIEQLGKPTLSHEIGQWCAYPNV
                     VSEPEKYRGFFRGSNVEVFRDILKKKGMWDQAEDFIKASGRFQVALYKQEIETALRTP
                     GMAGFQLLDLHDFPGQGTAPVGVLDAFWESKGYCTADEYRRFCNSMVPLARLKKRIFT
                     ADETLEFRIDLAHYGPRDLKSAVIEWELRQQSEVIARGELPPRDVPTGNVAEGDVLSV
                     PLSEIERLKVPTMLSLVARVKGTSWENDWNIWVYPRPAEPAQTEGITITRDPEEAWKL
                     AEEGQSVLLVPRPEIIAGDTLGTFQPIFWNRVTFPSQKVHMVGILCDPKHPALKSFPT
                     AFHADWQWQELLDACKPMVLDGLPKNLRPIVQAIDDWCEARKLGLVWEAKVGTGRVLV
                     CSIDITSDLTQRVVARQLRASLLDYIRSTPVQPLIALQREEWETLWREPPLLQKLGAK
                     ASADSFEPGFEPACAIDGDPKTMWHSAWTPEPAELPHWFVIDLQQPLTITGLRVLPRQ
                     DGNPNGQIAEFEVYVSRDGENWGEAVAKGTWDARPVERTIEFSSPVIARFIKFVARRE
                     IRGKQFACIAELDIIVSQ"
     gene            2637..>4088
                     /locus_tag="PKI05_05800"
     CDS             2637..>4088
                     /locus_tag="PKI05_05800"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOA51751.1"
                     /translation="MRISSNRWRLMWGLSATWLVLIVAGSLLADVKPVALFCDHAVLQ
                     QQMTIPVWGTADPGEEVTVRLGDQKVTTKADNNGDWMVKLQPLPAGGPYEMTIQGKNQ
                     IVLKDVMIGEVWICSGQSNMQWTVRNSANAEEEIANSANPMIRLFTVPRQAKPEPQKD
                     VVGAWAVCGPETVADFSAVGYFFGRHLQKKLGIAVGLINTSYGGTPAEAWTNWNKLAC
                     MPELTPIVDRFRDALAKYPENLKRYEEALKAHREKARQAKERGETFTQRAPSPPMGPE
                     HPHSPAGLYNAMIAPLVPYAIRGAIWYQGESNAGRAYQYRTLFPAMIASWRDAWNQGD
                     FPFLFVQLAPYMKIETEPTESAWAELREAQLLTMLNCRNTAMAVITDVGEEDNIHPKK
                     KQPVGDRLAIAARALAYGEDIEYSGPIYERMEIQGNKVILYFTHVDGGLVCKGDKLTG
                     FTICGEDRKFVNAEAKIEGDTVVVWSDQVEKPVA"
BASE COUNT          914 a         1048 c         1229 g          897 t
ORIGIN      
        1 cccgtcagtt ggtcccccga aggagcaaac gtgacctgtc gttaccatct gggcgagggg
       61 gctctcctat gggatgaatt ccatcctcat atctacgcac tccaggtaac gttggagaca
      121 gctcagactc gggacacagg ggatccggcg ggccttcagc acacgcggac ggtgaccttt
      181 ggccttcgcg atttggaggt tcgggggacg caattcgttc tcaacggtcg tccgatcttt
      241 ctgcgcggaa ccttggaatg ctgtatcttt ccgatccacg ggtatccacc gacagaccgc
      301 gaatcatggc gaaaggtttt tgccacggcg cgtgattacg gcttgaacca cctgcgcttt
      361 cattcctggt gcccacccca ggcggcattt gaggttgccg acgaaatggg tttctatctt
      421 caggtggaat gctcgtgctg ggcttcgttt ggggatggcg ggtcgcaaga tgcctttgtt
      481 tatgaagagg ccgagcggat tcgcagggct tatggcaatc atccgtcatt tctgcttatg
      541 gtggcgagca atgaaccggg cggacggaat tcaaatcagt tcttggccaa atgggtggag
      601 acgcagattg cggccgatcc acggcgttgt tattcggcgg ggtccggttg gccacagctt
      661 gcagtcaatc agttccacgt ccagcctcgg acacgattac aaaactgggg accgctccaa
      721 ctcaataagc caccgcaaac ctgggatgac tatcgcgaat acatagaaca attggggaaa
      781 cccaccctca gtcatgaaat cggtcagtgg tgtgcctatc ccaatgtcgt ttcagagccg
      841 gaaaagtatc ggggcttctt ccgtggctcc aacgtggagg tcttccgcga cattctgaag
      901 aagaagggta tgtgggatca ggccgaggac ttcatcaagg catcggggcg atttcaagta
      961 gccttataca agcaggagat tgaaacggcc ctgcgaacgc cgggcatggc aggttttcag
     1021 ctccttgatc tgcacgactt tccgggacag ggaacggctc ccgtcggtgt gctcgatgcc
     1081 ttttgggaaa gcaagggtta ttgcacagcc gatgaataca ggcgcttttg caacagtatg
     1141 gtgcctctcg cccggctcaa aaagcggatt ttcaccgcgg acgagacgct ggagttccgg
     1201 atcgatttgg cccattatgg ccctcgggac ttgaagagcg ctgtcatcga gtgggaactc
     1261 cgtcagcaat cggaggtgat cgcccgggga gagctgccac cgcgcgatgt tccgaccggt
     1321 aatgtggcgg aaggtgacgt cttaagcgtg cctcttagcg aaatcgaaag actcaaagtc
     1381 ccgaccatgc tgtcgcttgt tgcacgagtc aaagggacat cctgggaaaa tgattggaac
     1441 atttgggttt atccacggcc tgcagaacca gcacagaccg aggggattac tattacccgt
     1501 gaccctgaag aggcctggaa gctggctgaa gaggggcaat cggtcttgct ggtaccacga
     1561 ccggagatca ttgccggtga tacgttgggg acatttcagc cgatcttttg gaaccgtgtc
     1621 acattcccct cgcagaaggt tcacatggtg gggattctgt gtgatccgaa gcaccccgca
     1681 ttgaaaagtt ttcccacggc ttttcacgcg gattggcaat ggcaagagtt actcgatgcg
     1741 tgcaagccga tggtgcttga tggcttgcca aagaacctgc ggcctatcgt ccaggccatt
     1801 gatgactggt gcgaggcacg aaaactcgga ctcgtttggg aggccaaggt gggaacgggc
     1861 cgcgtgctcg tctgttcgat cgacatcaca agtgatttga cgcagcgtgt ggtcgctcgt
     1921 cagcttcggg ccagtttact tgattacatt cgatccacac cggttcaacc tctcattgca
     1981 ctgcagcgtg aggagtggga aacgctttgg cgagaaccac cgctattgca gaaactgggt
     2041 gccaaggcca gtgccgacag ctttgagccg gggtttgaac cagcctgtgc tattgacggc
     2101 gatcccaaga ccatgtggca ttcggcgtgg acgccagaac cggccgagtt gcctcactgg
     2161 ttcgtgatcg atttgcaaca accgctgacg ataacgggct tacgtgtcct cccacggcaa
     2221 gatgggaacc ccaacggcca gatcgccgag tttgaggttt acgtcagccg agatggcgaa
     2281 aactggggtg aggccgtggc gaaggggaca tgggacgccc gaccggttga acgaaccatt
     2341 gagttttcca gccccgtaat cgcccgattt atcaagtttg ttgctcgccg ggaaatccgg
     2401 ggaaaacaat tcgcttgcat tgccgagttg gacataattg ttagccaata acgagaaatc
     2461 cgagatcatc ggatagtggt gtgccctcag caccgcatca tcggtagtga gacgaaatga
     2521 ggcgttgcac gttgcgggtg cttgcggggt actctgattg gcgtcagaat aatcacaggt
     2581 ttttggggtg aaggcacgtc gttttgtttt ttcccacatg gagcgaggag gatgacatga
     2641 ggattagcag caacagatgg cgtttgatgt ggggactgtc ggcaacgtgg cttgtcttga
     2701 ttgtagctgg cagtctgttg gccgacgtta aaccggtggc gctcttttgc gaccatgcgg
     2761 ttttacagca acagatgaca atcccggtct ggggtaccgc agatcccggt gaggaagtaa
     2821 ccgttaggct gggggatcaa aaggtcacga ccaaggcgga taacaacggc gactggatgg
     2881 tgaaacttca accgttgccg gccggtggtc cttatgagat gacaattcaa ggaaaaaacc
     2941 agatcgtttt aaaagacgtg atgatcggtg aggtgtggat ttgcagtggg cagtccaata
     3001 tgcagtggac ggtaaggaat tccgccaacg ccgaggaaga gatcgctaac tcggccaatc
     3061 ccatgattcg tctctttaca gtaccgcgtc aagcgaagcc tgaaccccaa aaggacgtcg
     3121 ttggggcgtg ggctgtgtgt ggcccggaaa ctgtcgccga tttctccgct gtggggtatt
     3181 tctttggccg gcatcttcaa aagaaacttg gcattgccgt gggattgatc aatacttctt
     3241 acggcggaac acctgccgag gcttggacaa attggaacaa acttgcctgc atgccggagt
     3301 taacgcccat cgttgatcgc ttccgcgatg ccttggcgaa atacccggag aatctcaagc
     3361 gctacgaaga agcgctcaag gcccatcgtg aaaaggcccg gcaagccaag gagcgaggcg
     3421 agacattcac gcagcgggct cccagcccac cgatggggcc cgagcatccg cacagccccg
     3481 ctgggctgta caacgccatg attgcacctc ttgtcccata cgccatccgc ggggccattt
     3541 ggtatcaggg ggagtcgaac gccggccggg cctaccagta tcggaccttg ttccccgcga
     3601 tgatcgcgtc ctggcgtgac gcgtggaacc agggcgattt tcccttcctt tttgtgcagc
     3661 ttgcccctta catgaagatc gaaacggaac caacggagag tgcttgggca gagttgcgtg
     3721 aagcccaact gttgacgatg ctcaattgcc gcaacaccgc gatggccgtc atcaccgacg
     3781 tcggtgagga agataacatc caccccaaga aaaagcagcc ggttggtgac cgtttggcga
     3841 ttgcggcgcg ggcacttgcc tatggtgagg acattgaata ttccggcccg atctacgaac
     3901 ggatggaaat ccagggaaac aaggtgatcc tctactttac tcacgtggat ggtggccttg
     3961 tttgcaaagg agataagttg acgggcttca ccatctgtgg cgaagaccga aagttcgtca
     4021 acgccgaggc caagatcgaa ggtgacaccg tcgtcgtgtg gagcgaccag gtcgagaaac
     4081 cggtggcg
//
LOCUS       DAOPTR010000239         4076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_17676_length_4076_cov_2.806018, whole genome shotgun sequence.
ACCESSION   DAOPTR010000239 DAOPTR010000000
VERSION     DAOPTR010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17676_length_4076_cov_2.806018"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3102)
                     /locus_tag="PKI05_05805"
     CDS             complement(<1..3102)
                     /locus_tag="PKI05_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51752.1"
                     /translation="MVRAWSWTILVFIAVVFSLGALDYVLRFQERGLRFLATGTLLAT
                     TVWCFFRIVKPAMTLSIDGIALARWLEKFQGDAPVSLAAACDLVRLPLDDLRWGSPAL
                     RSMAIEQAEAHWTVPPSREVIERGPALWPSYLAAAALIVGFSLLLVFPVVAGTALIRL
                     VNPLSDLSYPQTTHLVVVHYPERLSRGDTFFLQVTEERGQLPDEVRIAIRPLDGESHR
                     AVNQLMKKVRNQFVFRQENVRTSFQFLITGGDDRSQPWRRVEVVDPPQVSEWKVWLKP
                     PEYTGWPPEEARGPLRALAGTEVQLSGRANRPLKRVVVDCGPQQSFQGDVLPDQYSFR
                     VPGGKNHRPEQERWILQTSGYYRIRLEAADGTFSVNEPRWEVRVYEDAPPTVVLESGQ
                     RLLVMTPDGRLPLRVSARDDLAIRQINLVSRDSSGNSLGPTIPLYQGPPFPPTQIRMA
                     ELETHADSVEKEYLFRLPVSAGNTGLTFRVVASVEDYKGQASESEPLEIRVISPDEFN
                     QRLASEEEKLLQQLAQLYESFVKISRDWERLSEKLRAEKRLSRTEANQLLTLDTLERQ
                     AFRRIADPGLGILYELQDLAGMAEYQGVTQDRVERLKKFRTDLEASWKQHGVPAESQL
                     GMIANWARNLVERLLGDEQSLSIEKEWADVAQNADQFSLHQAAWEKALEGLLQNVSGI
                     RLVNTLRRELEKLLTEQLELIQQTRDLGQQTLGRALQDLTPNEQDALTRIARRQTLLA
                     TKLEELVAKVLGGLQQSGNQDVTRQGFRPHEGSMSEREDDLTIQSSQMVSQQGAVSQQ
                     SFEKPKGDAVPKEPQLIALIIDRINGHGLTALMRQAGLAIEQNRLSEGLTLEEQSAEI
                     LRELIDLLDGKREDAGRRQQDLAEQENELRNLLRAQQDLIRKFAEARQTAVEEALSTF
                     IREQSKIEQRTRELADQMAPPLKRVAEPLLKEAIRRMQDAQTSARQKDVGKTEQNATL
                     AQGLMEQALEAVGQERITQERNKAYDRWHEFVRRVNGIIGEQRDLLRKTQSLDIEKRK
                     SQTP"
     gene            complement(3203..>4076)
                     /locus_tag="PKI05_05810"
     CDS             complement(3203..>4076)
                     /locus_tag="PKI05_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51753.1"
                     /translation="AVTREGLVMPWLAFGRSPTETERVWHSLPPVYWLQSVIELKPGA
                     RVLVESREENQQEGRGEPVVVFQYVGAGKVLFHATDETWRWRRGAGLAAYRHYWIEAV
                     RFMARGKLESARTPRLVTDRSEYQAGDSVHLMLECPKPAELPLRNGSIAIWLEYEGRT
                     RWEVTLQRHPLRGDLFEATLQNLAPGQYYAWVPITSGIVPATGFAVVPPLQEWEATQM
                     ARKEMETAARLSGGGFYLPSEIRRLRDDLPHGRGALIEMLPPRPLWNSPIVLLLLVLT
                     LSLEWLLRKRYGLL"
BASE COUNT          892 a         1206 c         1031 g          947 t
ORIGIN      
        1 cggtgtttgg gattttcgtt tctcgatatc cagcgactga gtttttctca gaagatctcg
       61 ctgttcgccg attatcccat tcacgcgtcg cacaaactca tgccatcgat catacgcctt
      121 gtttcgctct tgtgttatcc gctcttgtcc cacggcctca agcgcctgct ccattaagcc
      181 ctgagccaat gtcgcgttct gttcggtctt tccaacatct ttttgccggg ccgaggtttg
      241 agcatcctgc atacgccgga tcgcttcttt caacagcggt tcggcaaccc ttttcaaggg
      301 tggcgccatt tgatccgcaa gttcccgcgt tcgttgctca atctttgact gctcacgaat
      361 aaacgttgat aaagcctctt caactgccgt ttgccgtgct tccgcaaatt tgcgaatgag
      421 gtcctgttgg gcacggagaa gattcctcaa ctcgttttct tgttcggcaa ggtcttgttg
      481 acgtcgcccg gcgtcctctc tctttccgtc taatagatcg attaactctc tcaggatctc
      541 ggcagactgc tcctcaaggg ttaggccctc gctcaaacga ttctgttcaa tggcaagccc
      601 cgcttgacgc atcaaagccg tcaatccgtg cccattgata cgatcaataa ttagcgcaat
      661 caattgtggc tcttttggga cggcgtctcc cttcggtttt tcaaagcttt gttgggatac
      721 tgccccttgt tgcgaaacca tttgactgga ttgaattgtc agatcgtcct cgcgctcact
      781 catcgaccct tcatgtggcc gaaatccctg acgggtaaca tcctggttcc cgctctgttg
      841 gagccccccc agcactttag caaccaactc ctcaagcttg gttgcgagaa gcgtttgacg
      901 ccgcgctatt ctcgtgagcg catcctgttc gttgggcgtg aggtcttgaa gagctcgtcc
      961 taacgtctgt tggcccaagt cgcgcgtctg ttggatcagc tccaattgtt cggtgagaag
     1021 tttttccaat tcccttcgca gggtgttgac cagccgaatg ccgctaacat tttggagcaa
     1081 cccctccaac gccttttccc aggccgcttg gtgaagcgag aattgatcgg cattttgtgc
     1141 gacatctgcc cattccttct ctatgctcag cgactgttca tctcccaaga ggcgctccac
     1201 caagttccta gcccaattgg cgatcattcc aagttgcgac tccgcgggaa ccccatgttg
     1261 cttccaactc gcttccaaat cagtgcgaaa ctttttcaat cgttcaacgc ggtcctgagt
     1321 cactccctga tactcggcca taccggcgag atcttgcagc tcataaagaa tccccaaccc
     1381 cgggtcggca atccgccgaa aggcttgtcg ttccaaggta tcgagcgtga gaagttgatt
     1441 ggcctctgtt cgcgagaggc gtttttcggc acgtaatttt tcggaaagcc tctcccaatc
     1501 gcggctgatc ttcacaaacg actcgtaaag ttgggcaagc tgttggagaa gtttctcctc
     1561 ttcagaagcc agtcgttgat tgaactcatc tggggaaatc actcgaatct caagcggctc
     1621 actctctgat gcttgtccct tgtaatcttc gacacttgcc acaactcgaa acgtaagccc
     1681 ggtgtttcct gccgatacag gtaatcgaaa caggtattcc ttttcgaccg agtccgcatg
     1741 tgtctcaagc tccgccatac gaatctgcgt tggcggaaag ggaggcccct ggtagagagg
     1801 aatcgtgggt ccgagagagt ttccactgct gtcacgactc accaggttga tttgtcgaat
     1861 ggccaggtca tcccgcgcac tgacgcgcag cggtaatctg ccgtcgggcg tcatcacgag
     1921 caatcgttga cccgactcga ggaccaccgt gggcggagca tcttcataca cgcggacctc
     1981 ccagcggggc tcattgaccg agaacgttcc gtcagcggcc tccaaccgga ttcggtagta
     2041 cccgctcgtc tgcagaatcc atcgctcctg ttcgggtcga tggttttttc caccaggcac
     2101 gcggaaagaa tattgatccg gcaggacatc gccttgaaag ctttgttgag gtccgcagtc
     2161 caccacaaca cgctttaagg gccggtttgc gcgaccggac agttgcactt cagtgcctgc
     2221 caatgcccga agcggcccgc gagcttcctc aggcggccag cctgtgtatt caggcggttt
     2281 gagccacacc ttccactcgg aaacctgggg aggatcaacc acctccactc ggcgccaagg
     2341 ctggctacga tcgtcacccc cggttattag aaactggaaa ctggtgcgaa cattttcctg
     2401 gcgaaatacg aattgatttc gaactttttt catgagttgg ttgaccgccc tgtgagattc
     2461 gccatcaaga ggccgaatgg caattctcac ttcatccggc aactgtccgc gctcctccgt
     2521 aacttgaagg aaaaacgtat caccgcggga taatcgttct ggatagtgaa cgacaaccaa
     2581 atgagtcgtc tgaggatagg aaagatcact caaaggattg acaaggcgga tcagtgctgt
     2641 tcccgccacc acgggaaaca ccaggagcaa agaaaacccg acgatcaggg cagccgcagc
     2701 aagatagctc ggccaaaggg ctggacctcg ttcaatcacc tcccgcgagg gcggtaccgt
     2761 ccagtgcgct tccgcttgct cgatcgccat ggaccgcaat gctggcgagc cccacctgag
     2821 atcatccaac ggaaggcgca cgaggtcgca tgccgcggcc aacgacacgg gcgcatcacc
     2881 ttggaacttt tccagccagc gcgcaagggc aatcccgtcg atggagagcg tcatcgccgg
     2941 tttgacaatt cggaaaaagc accacacggt ggttgccaat aatgtgccgg tggccaaaaa
     3001 gcgcagaccg cgttcttgaa accgcaaaac atagtccagt gccccaagcg agaacaccac
     3061 ggcaataaaa acgagaatcg tccagctcca cgcgcgcacc attaccctgt ggcgaagtcg
     3121 ccggcgtaaa gtttcaacgt gccgcgcaaa tttcggtgtc atcattgtct caggacaaag
     3181 cgaaactcct cctgtcgtta ttctacaaca agccatatcg cttccgcaga agccattcta
     3241 aggaaagcgt gagaaccagg agcagcaaga caatcggaga gttccacaag gggcgtggcg
     3301 gcagcatctc gatcaatgct cccctgccgt gaggcaaatc gtctcgaaga cggcggattt
     3361 cgctgggcaa atagaagccg ccgcctgaaa gtcgcgccgc ggtttccatt tcctttcgcg
     3421 ccatttgtgt tgcttcccac tcttgaaggg gcgggacaac ggcaaaacca gtcgccggaa
     3481 caatccctga ggtgatggga acccaggcgt agtattgacc tggggcaagg ttctgaagcg
     3541 ttgcttcaaa caaatcaccc cgcaaaggat gacgttgcaa ggtgacttcc caccgagtac
     3601 gtccctcgta ttccaaccag attgcaatgc tcccatttcg caggggcagt tccgcgggct
     3661 taggacattc caacattagg tggacagaat cccctgcctg atactcggag cggtcagtaa
     3721 caagccgagg tgttctcgcg gattccaatt ttccccgcgc cataaagcgg acagcctcga
     3781 tccagtagtg gcgatatgca gccaggcctg cccctcggcg ccagcgccag gtttcatccg
     3841 tggcatggaa caggaccttc ccagcgccaa catattgaaa gactacgacc ggctcgccgc
     3901 ggccttcttg ctggttttct tcacgagact cgaccaaaac ccgtgccccc ggtttgagct
     3961 caatcacgga ttgaagccaa tatacgggcg gtaacgaatg ccacacccgt tcggtctcgg
     4021 tgggtgatct tccaaaggcc agccaaggca taactaggcc ctcccgagtg acggcc
//
LOCUS       DAOPTR010000240         4049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_17819_length_4049_cov_4.031047, whole genome shotgun sequence.
ACCESSION   DAOPTR010000240 DAOPTR010000000
VERSION     DAOPTR010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17819_length_4049_cov_4.031047"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PKI05_05815"
     CDS             <1..763
                     /locus_tag="PKI05_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51754.1"
                     /translation="PAARFDGLSLAEVLRNPQANLPDRMMVVQYGGRTRAEKNDCCVI
                     WGNWRLVKGSELYDVSRDLAQEQDLSQVHPDVVKKMQSFYDHWWTQIEPTVYEYQPVV
                     VGTEQENPVILSSNYWAGVDVDNHHRVSAGEGGPRGGPYHIDVATPGRYRIELRRWPF
                     HCNEPLGSAGPKQTVAGRSLEEVIRNRKALPIAGAVLVIGNQEFQKQTSPEAMGITFE
                     VSLPAGRTILQGWFLNSQGEDLCGAYYVSVEKLAN"
     gene            838..>4049
                     /locus_tag="PKI05_05820"
     CDS             838..>4049
                     /locus_tag="PKI05_05820"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOA51755.1"
                     /translation="MKNGSFCVAVGLIVVLSHVGVTDCADEPLTTSTAVKSLLAKPPR
                     QFSSAPLWVWNDLLTDEQIVSTLHDLAGQDVKQVFVHPRPGLITPYLSDEWFRLWKLA
                     LKTAESLDMNVWIYDENSYPSGFAGGWVPEVMPESIGRGLAVKETGNLAEVPPSAVAV
                     YRLTNDGYENVTQQFRAKEQLGGNQYLYAEVVRSAPSPWHGGRPYVDLIYPGVTQKFL
                     EVTLDAYQREVGEWFGSRIPGSFTDEPRLLAAGQLSWTDDLADRFWETYGYDLLDHLP
                     SLIRPVGDWKRVRHDYYRLLLHLFIERWAKPYYQWCERHNLEFTGHYWEHEWPRCRFV
                     PDNMALYAWHQRPAIDCLMNQYAEHTHAQFGNIRAVRELSSVADQLGRQRTLCELYGA
                     GGWDLRFQDMKRIADWLGVLGVNTFDEHLSFISIRGARKMDHPQSFSYHEPWWDSYYV
                     IARYLKRLSLVLSSGERVNEILVIEPTSTAWMYQGQTPNKTELSLLGSSFFELLKSLE
                     AAQIEYDLGCEDIMARHGSVADGRLIVGSRHYHTVVLPPGCENLESTTVELLERFLKL
                     GGHVLVCGESPSLVNATPSQRIDSLKRLPGWKKCDPTDLPLILSERQDPRIRIIREKD
                     DRGLLFHQRRRLADGEIVVLVNTSDTESTRGVIETSYAGAEIWDLEEGNTRPAHFVRK
                     DNRLLLPFDLSPVGSLVVFFSQKETVSGPAQEFQEQPVSLSPISIARLQPNVLTLDYV
                     DIQVGSELRRGIYYYQACDWVFKKHGLPGNPWDRAVQFKDEIIRQSFPPETGFTLTYR
                     FQIRESVPSDLAIVIERPDLYAITCNGQPVSWNGKDWWLDRAFGKIDISRSVRVGENE
                     VQLVARPFTLFHEIAPAYVLGDFSLEPAEKGFVIVPAKPLQIASDPGAHTAQPNHTMW
                     LSAGIGFDRSAAPADDPQPWLVFDFGRTVSLAVIQIWNYNEVNLTSRGVKDLEIVLLD
                     DAPSLEAGKVIARVTLPQALGRETVSGTSGLPATIAIPETKVRRLGFRILSNHAGVTF
                     PADKPAVDNAFVGLSEVRFLSKKDNALEEIKGVQVACSSEL"
BASE COUNT          872 a         1053 c         1132 g          992 t
ORIGIN      
        1 gcccgcggcg cggttcgatg gactaagcct ggccgaggtc ctgcggaacc ctcaggcaaa
       61 tcttcccgat cgcatgatgg tcgtccaata tggtgggcgg actcgggcag aaaagaacga
      121 ctgctgcgtc atctggggga attggcgact tgtcaagggt agcgaacttt acgacgtgtc
      181 gcgtgacttg gctcaagagc aagatctcag ccaggttcac cccgacgttg tcaagaaaat
      241 gcagtcattt tacgaccact ggtggaccca gattgaaccc acagtgtatg agtaccaacc
      301 tgtggttgtg ggaacggagc aggagaaccc agtgatcctt tcatcaaact attgggctgg
      361 agttgatgtg gataatcatc accgcgtttc cgcaggtgaa ggcggtcccc ggggtggtcc
      421 ttatcacatc gatgtggcca caccgggacg ttaccgaatt gaactgcgtc gctggccgtt
      481 ccactgcaat gaacccctcg gttccgcggg cccaaagcaa accgtggcgg gacgttccct
      541 ggaggaagtc atccgcaacc gaaaggccct gcctatcgcc ggtgcagtgt tggtgatcgg
      601 taatcaggaa tttcagaaac aaacttcacc cgaggccatg ggcattacct tcgaggtatc
      661 cttgcccgct gggcggacga tcctccaggg gtggtttctt aatagccaag gcgaagacct
      721 ttgcggggct tattatgtca gtgtggaaaa gctggcgaat tgatggtcac catgtcgctt
      781 accgtgatct tcgatgtgct tgcctggtga ccattagtgc gaatgggaga atcttgtatg
      841 aagaatggaa gtttttgtgt tgctgttgga ttgatcgtgg tcctatcaca cgttggggtg
      901 acggattgtg ccgacgagcc tttgacgaca tccactgccg ttaagtccct tttggccaaa
      961 ccgccccgtc aatttagctc ggcgcctttg tgggtatgga atgacctctt gaccgatgag
     1021 cagatcgttt cgaccctgca tgatttggcg gggcaagacg tcaaacaggt gtttgttcat
     1081 cctcggcccg gcttgattac gccttatttg tcggacgaat ggttccgctt gtggaaacta
     1141 gcacttaaaa cggccgagtc tctcgacatg aatgtctgga tttatgatga aaactcctat
     1201 ccttcgggct ttgccggggg atgggtacct gaagttatgc cggaatcaat tggccggggt
     1261 ctggcagtta aagaaacagg caatctagcc gaggttcctc cttccgcggt tgctgtctat
     1321 cgtttgacga acgacggtta tgagaatgtc acccaacaat tccgagccaa ggaacaactc
     1381 ggtggcaatc agtatctcta tgccgaggta gttcgttccg caccatctcc ttggcacggc
     1441 ggacgaccgt atgtggactt gatttacccg ggcgtcacgc aaaagttttt agaagtcaca
     1501 ttggacgctt accagcgcga agtaggagag tggttcgggt cacggattcc cggttcattc
     1561 acggatgaac cgcgattgct tgccgccggg cagctttcat ggacggacga tttggcggat
     1621 cgcttttggg agacctacgg ctatgacctt cttgaccatc taccctcgtt gattcgcccg
     1681 gtgggcgatt ggaagcgggt acggcatgat tactatcggt tactgcttca tttattcatc
     1741 gaacgctggg ccaagccgta ttaccagtgg tgcgagcggc acaatcttga gttcaccggc
     1801 cattactggg aacacgaatg gccacggtgt cggtttgtgc ccgacaatat ggccctttat
     1861 gcctggcatc agcgtccggc gattgactgc ttgatgaacc aatacgccga acatacgcac
     1921 gcccaatttg gcaatatccg tgctgttcga gaactttcga gtgtggccga tcagttagga
     1981 cgacaacgta cactctgtga gttatacggg gccgggggct gggacttacg cttccaagac
     2041 atgaaacgga tcgccgattg gctgggcgtt ctgggcgtca acactttcga cgagcatctt
     2101 tcgtttatct ccattcgagg cgcgcgaaag atggatcatc cccaatcgtt ctcgtatcac
     2161 gaaccatggt gggattctta ttatgtcatc gctcggtatc tcaagcgact ctccttagtc
     2221 ctttcttccg gtgagcgggt caacgagatc ctcgtgatcg agccgacctc gacggcctgg
     2281 atgtaccagg gacaaacccc aaacaaaacc gaactgtcgc tcctgggaag tagtttcttt
     2341 gaactgctca aatctttgga agcggcccaa attgaatatg acctgggatg tgaagacatc
     2401 atggcccggc atggtagcgt cgctgatggg agattgatcg ttggatctcg gcattaccac
     2461 acggtggttt taccccctgg gtgcgagaac ctcgaaagca ccacggtgga gctcctcgaa
     2521 agatttctga aacttggcgg ccacgtgctt gtttgcggcg aaagtccttc ccttgtcaat
     2581 gccacaccgt cccagcgaat tgattcccta aaacgactcc caggttggaa gaagtgtgac
     2641 cccactgact tgccgctgat tctttcggaa cgtcaagatc ctcggattcg cattattcgc
     2701 gaaaaagacg atcgggggtt gctctttcac cagcggcggc gcttggccga tggcgagatt
     2761 gtcgttttgg tcaatacaag tgataccgaa tccacccgtg gtgttattga aacgagctat
     2821 gctggggccg aaatttggga tttggaagaa ggtaacaccc gtcctgctca cttcgtgcgg
     2881 aaggataacc gtctcttact ccccttcgat ttatcgcctg tcggtagcct agtggtgttc
     2941 ttttctcaga aggaaacggt atccggtccc gctcaagaat ttcaagaaca accggtgtcg
     3001 ctgtcaccga ttagcatcgc gcgcttgcag ccgaatgtct tgacgctcga ttatgtcgat
     3061 atccaggtcg gctcagaact tcgacgcggc atctactact accaggcttg tgattgggtc
     3121 ttcaagaagc acgggctgcc gggcaatcct tgggatcggg ccgtccaatt caaggacgag
     3181 attattcgcc aatccttccc accagaaact gggtttacct tgacctatcg ctttcagatt
     3241 cgtgaaagtg tccccagtga tctggcgatt gtgattgaac gcccagacct ttacgccatc
     3301 acctgcaacg ggcaaccggt ctcgtggaat ggtaaggatt ggtggctgga tcgtgcgttt
     3361 ggaaagatcg acatctcccg atcagtacga gtcggtgaga atgaagtgca gcttgtggca
     3421 cgcccattca cgcttttcca cgagatagcc ccggcctacg tgttagggga tttctctctc
     3481 gagccagccg agaaaggttt cgtgatcgtg cccgccaagc cgctgcagat tgcctccgac
     3541 ccaggggcgc acacagctca gcctaatcat acgatgtggc tatcggcggg aattggcttt
     3601 gaccgctctg ctgcgccggc cgatgatcct caaccgtggc tcgttttcga tttcggtagg
     3661 actgtgtctt tagccgtcat tcagatttgg aattacaacg aagtcaattt aacatcccgg
     3721 ggcgtgaaag acctggagat tgtgcttttg gacgatgccc cgtcgctgga ggctgggaaa
     3781 gtcatcgccc gtgtcacgct tccgcaagcc ttggggcgag aaaccgtcag tgggacatcg
     3841 ggacttcccg ccacgattgc gattcccgaa acgaaggttc gacgcttagg ttttcggatc
     3901 ctctccaatc acgcgggcgt gacattcccc gcagacaaac ccgcggtgga caacgccttt
     3961 gtggggctaa gcgaagttcg ctttctgtcc aagaaggaca acgcgctcga ggagattaaa
     4021 ggggttcagg ttgcgtgctc aagcgaact
//
LOCUS       DAOPTR010000241         4043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_17851_length_4043_cov_2.720662, whole genome shotgun sequence.
ACCESSION   DAOPTR010000241 DAOPTR010000000
VERSION     DAOPTR010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17851_length_4043_cov_2.720662"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PKI05_05825"
     CDS             <1..349
                     /locus_tag="PKI05_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51756.1"
                     /translation="KYGLWGAGKGAAVGAAIGGAAWGIGRVTGWIRRLPEQPEGLPDP
                     REEGILIRTKPRRGDYGNVEVEPGYARHHKKPLSLGGTDDPSNIVKVPVEIHRQPHPG
                     REVIEAPLGTIFY"
     gene            465..881
                     /locus_tag="PKI05_05830"
     CDS             465..881
                     /locus_tag="PKI05_05830"
                     /inference="COORDINATES: protein motif:HMM:NF020904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HOA51757.1"
                     /translation="MTWPEQLLRDRGELLPPADPGVIEEVARSLGIHFPQEYVEFLLW
                     ADGGILPGKRFLLYSVGPGLHPAETILAANRDRPADFPLLLIGRDAFEEFGFLKAELV
                     APKPTSPLYLWLHETEATKQLASSFSAFIRSIVTKD"
     gene            1254..1544
                     /locus_tag="PKI05_05835"
     CDS             1254..1544
                     /locus_tag="PKI05_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51758.1"
                     /translation="MLQNYKSGNNSDGKLTTHQSVDQIVRSIYDIVRWDICAEARQNV
                     PTLTRLLFSRILDAREQHETQNPEAVGVACSPLGGTLSLARLGCTIGRGNNH"
     gene            1941..2261
                     /locus_tag="PKI05_05840"
     CDS             1941..2261
                     /locus_tag="PKI05_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51759.1"
                     /translation="MVSRLSFFYPAGIDVVDVSGSMTMTYVEAVSSIGTESGTVDDDS
                     PNSDGCDVCGRFTIGNPSGWDNEAALATRERLAALWPRLPLEVQRQIVAIAEESVADR
                     ERNR"
     gene            complement(3161..3502)
                     /locus_tag="PKI05_05845"
     CDS             complement(3161..3502)
                     /locus_tag="PKI05_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51760.1"
                     /translation="MSPYEALLRSHHLDVDKPGMCVEKDGYGGIMVYQTYPGTDLPDF
                     SKPAICVEKDGYDRMMVYQTYPGTNIPDSRKPGIYVERDDDGRMMLYETYPGTNIPNL
                     RKPAIRIESDY"
BASE COUNT          920 a         1114 c         1097 g          912 t
ORIGIN      
        1 aaagtatggc ctatggggag ctggcaaagg cgccgctgtc ggggcagcga taggtggcgc
       61 agcctggggt ataggccggg tcactggctg gattcgtcgc cttcctgaac aaccagaggg
      121 acttcctgat ccgagagagg agggtattct cattcgcacg aagccacgtc gtggtgacta
      181 tggaaatgtt gaggttgagc ctggctatgc gcggcaccac aagaaaccct tgtcattggg
      241 gggtaccgac gacccgagta atatcgttaa agtgccggtt gagattcatc gacaacctca
      301 tccggggcgc gaagttatag aggctcccct aggcactatc ttctactgat cggaccttgg
      361 agcggttgcc ccgtgaaact ggagcagcag gcaatctagg tcgtaacttg gatcgaccag
      421 cctaaaagac gtattcacat ctcgcagagg aaggaacgtt aatcatgact tggccggaac
      481 aattgttgcg tgaccgaggc gaactattgc cacctgcgga tcctggtgta attgaagagg
      541 ttgctcgctc actgggcata catttccctc aggagtatgt cgaattcctt ttgtgggctg
      601 acggaggcat acttcctggc aaaaggttcc tcctttactc cgttggtcct ggcttgcacc
      661 cagcggagac gattctcgct gccaaccgag atcgaccagc tgactttccc ttgctgctta
      721 ttggaaggga tgcttttgaa gaattcggat ttctcaaagc ggagcttgtg gcgccgaagc
      781 cgacgagccc actctatctc tggctacacg aaaccgaggc tacgaagcag cttgcatctt
      841 cattctctgc gtttattcgc agtattgtga caaaagatta aggcctcttt ctcagaattt
      901 gtgggtgggt tttgggcgta aacaccactg aaatccgagg atttctgatt tttcgaggtt
      961 cttcgaagcc gacaatttgg ggcacaaatt cctgcggttg tttctcggtc tgcaggagaa
     1021 cgggcagggg ccgggtacca cgcgatctcg gccgctaaac cgcccaaaaa gcccggcaat
     1081 aaaagcgttt tctcgtcatt tatttgcccc gctatgcatc aaaaaacgca cgtttcgacc
     1141 actgagggac tgggatttca ccctcaaatt ctcccgaaga cccttggata tagttctccg
     1201 agcggaagcc aaagccataa gggcaatacc tggactgata ttacatcggg tgtttgctgc
     1261 agaactataa gagcgggaac aacagcgatg gcaaactgac cacgcaccag tcggttgacc
     1321 agatcgttag gagtatctac gacatcgtgc ggtgggacat ctgcgccgag gcgcgtcaga
     1381 acgtgcctac actgacccgg cttcttttct cacgcatcct ggacgcgcgg gagcagcacg
     1441 agacccaaaa tcccgaggcc gtgggcgtcg cctgcagccc cctcggaggt accctatcgc
     1501 tggcgcgact gggctgcacc attggacgcg gaaataacca ttgatgtgaa cggcgaagaa
     1561 tggctggaag cgattcgcac ttgatgacgc aagttattat ttggaagatg cttgcgcagt
     1621 tgacgctgtc aagctgctgg acatagtgct gcactttctg cgcgggagcg gatggctagg
     1681 cttcccctgg accaacgaac cgggtttgtc cacgcacaac ggggatgttg aaccgacgag
     1741 cgccgaccgt taaggaaccc acaaagtagg ggctttctca tgacctatat cttgcgtaat
     1801 ttgagtggcc aagggtgggc gccgcgagac gtggtatgat ttggtggggt ggcggtggag
     1861 gagacggcgc ccaccccctc cgtcttccat gggtccttgc tagccgaaaa ctttcaaccc
     1921 tgccgccggg accagtcgca atggtgagca gactttcttt tttttatccc gcgggaatcg
     1981 acgttgttga cgtaagtggc tcaatgacca tgacttacgt cgaggctgtc tcctcaattg
     2041 gcacagaatc cggcacagtt gacgacgatt caccgaacag cgatggatgt gatgtctgtg
     2101 ggagattcac catcggcaac cccagcggat gggacaatga agctgcactt gcaacacggg
     2161 agcggttagc cgctctgtgg ccacggttgc ccttggaagt tcaacgccag atcgtggcga
     2221 tcgcggagga atctgttgcc gaccgagaaa ggaatcggtg aaacggcacg cctcaccaca
     2281 accggtgttg ctttggcaaa cacgcgccgg cttggacgcc gttttggcga agcggcattc
     2341 ggttgtttct cggcatgcat gaaaagggta gtgcctccac tgaactaaca gcctatctcg
     2401 aagagtcggc agcgcggatt gggagccacg tgcagatgaa tacccgcgcg caggcgtgat
     2461 agcaaagggc caagggcagc gtagttttcc gccttctttt cccagcgttc ccaacgaaat
     2521 cagcagcctc cggaaaggat tgaaccaaaa acgtgcgctc gattggttca atccatagga
     2581 ataggcccag cttttgacct cgcagaggga acttgctcaa caacccgtcg agggcccgcc
     2641 attcccggtg aatactttgt aactcgtcgg gttccccgag tggtatcttc tagagaagct
     2701 cgatcgcccc acaccccaca agcttctcaa gaagccggaa ccgaactccc cggctccact
     2761 tttcactggc agcctgtaaa gagctcattg aaaatttact tgaccccttg ccgccactcc
     2821 aaatcactta agccccggat aacggccccc tcgcttccat tacggaagca gaggttccat
     2881 gtggtctacc agacctcctt cgctaaccgg gttgccacag catccgatac tgtgactcag
     2941 agttttcttt cgagaaacgt cattttggga taagccccaa acccgttgac gagaccccga
     3001 aactcactct tgacgacttg gcttccgctt cggcaacgtt cgcaatggag attcgtgatc
     3061 gcctacggac agcggatttt gcgttaggcg gcaaagtcag agtaatcctg catcagcaaa
     3121 gcattccggg cgggtggcga cccctgggcc aacaaatgcc ttaatagtcc gattctatgc
     3181 gtatcgctgg ctttctcaaa ttcggaatat tcgtccccgg ataggtctca tacaacatca
     3241 tacgtccatc gtcatccctt tcgacatata tccccggttt cctagaatcc gggatgttcg
     3301 ttcccggata ggtttgatac accatcatac ggtcatagcc atctttttcg acgcaaattg
     3361 ccggtttgct gaaatctgga agatcggtgc ccggataggt ctgatacacc atgatacctc
     3421 cgtaaccatc tttttcgacg cacatcccag gtttgtcaac atcgaggtga tgcgacctca
     3481 gaagggcctc atatgggctc atcctctccc acctatatgg ctgacgcgga atcgaagtac
     3541 ccatgtaccc acgcactcca atgctcacag gggtagcggg gcttttgcgg ggaggagcag
     3601 gcccgtccga tttgtcgcca ccttgcaaaa gactcattgc gagcaccact acagcaacac
     3661 cgagaggact caaaccggat ccatctgcaa gccggtcgaa tagcgacata acagagtctc
     3721 ccgtaatgct attggccacc ggatcgtcat gcctgtcgtg acgctgcgag ggcacggcac
     3781 caagcgcaga ttatcgtaaa taggagcata ctccctgtca agcgctcggc tttctgggtg
     3841 cagctacgac ggctgcaagt tgcacctcac agggaagtcg tccaccctaa tgaaggtttg
     3901 tgagtggctt caattcgcca cgatggcttg ccgcgctgca ttgcggaggt ggttgactgc
     3961 ccgagacagc ccgcgacaca ttcctcccgc ggaggtaaaa atcctcgccc agatccgttt
     4021 ctgacagcgg cggcttgact cgt
//
LOCUS       DAOPTR010000242         4038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_17871_length_4038_cov_2.834296, whole genome shotgun sequence.
ACCESSION   DAOPTR010000242 DAOPTR010000000
VERSION     DAOPTR010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17871_length_4038_cov_2.834296"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2120)
                     /locus_tag="PKI05_05850"
     CDS             complement(<1..2120)
                     /locus_tag="PKI05_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51761.1"
                     /translation="MNRLKNVTTLIGLVLLAASSLSTVRLRSTFAVEAMHDTQKENAP
                     GVSWHHPLYLHWGGYWKSRVRVTYHNHWSRDLAGYPVALPVVCPGSLSAGAASGIPLA
                     GRNIRDIRVCTADGTELLWAACDEDMVSFTEGTFPPKGWLVVPVTCPARGKTEFFVYF
                     DNPEAIEVPDYFSAKPCPINCGVEKGEGETPSGWQHDASGPDRRAYWSNENPHSGAKC
                     LAIEVQPGSKPSWIATRQTGVAVVPGVRYRFRAWVRAENVEGYAGWYIHVGNAENPMM
                     IAPTISGGGGTYDWKQVEAEFTAPEGATTASLGTALWGTGRAWFDDVEWEMDPQACEV
                     VIHSPESSALAEVDETQAWPSSQENHFLGNQRLHLELLNFDTQARQNVLACLGPNVWG
                     GRLPLLAETDRVRMFAGTTRIKPFVVGEYLLWPDHLPARSRVHRYVYCEDPTSVQPIS
                     VQPIEEKDATNKVLLEYMALLNSSNNLVQNSSFETGGDIPEGWVQSGAGDGRVKFSVE
                     DPKHEGLGRRAARLDIPSGTPSAWRGWQQRIPVKAGTTYLVAGWLKCQDLEGEARIHI
                     HFHDSQGRLCKSGGMTSVGPGVRATQPWTLVSGLVKTPNDAASLTLHLTTNVSGTLWH
                     DAIFVAEVCNVSCVKREAAPMAKDAFVVWQVPAVVKVFREDPPVRERQSIQVSVAKGE
                     WEALQLAVRSGTSLAEVRVEVLPP"
     gene            complement(2301..3404)
                     /locus_tag="PKI05_05855"
     CDS             complement(2301..3404)
                     /locus_tag="PKI05_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOA51762.1"
                     /translation="MSQDKLRRRIAWEAARLIYFREETEYQRARTKAARRICRGEAPL
                     GDLPTNREIREQIEAVARAFEGERRGVEVGEMRLEALRIMQILKEYQPYLVGGTLTGH
                     ARRGSPIDIHIFSDSVEAVQDTLQKSHIPFEIVNRPGETAGSEKSCVCFHVTSRFVCR
                     LTLYPAAMVGQLPINVTTGQPIQRANIGELERLIAREHPALVYRTCPQEEPALVDRFL
                     AYESLLVPLEHIRDDRKGQPDIDLLTHSLQVFHLARSESPYDEEFLLAALLHDVGKAI
                     DPKDHVAAALEALDGYITERTAWLIAHHMEGLQVLDGSIGQRARRRLESSEDYDLLVL
                     LARCDRAGRQVGVAVPDLEDAIEYLRALDRDFG"
     gene            complement(3754..>4038)
                     /locus_tag="PKI05_05860"
     CDS             complement(3754..>4038)
                     /locus_tag="PKI05_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOA51763.1"
                     /translation="IFLEGTPIVSQVGDVHVGPDVQTQLQSDLNAEMGAIQAYNKAIQ
                     LAIDLNDSVTRDLLESIAKDEDDHINWIEEQLAQIEQMGLPMYLTTQTKD"
BASE COUNT          912 a         1183 c         1037 g          906 t
ORIGIN      
        1 ctaggaggca gtacttcaac ccgcacctcg gccagtgacg tgccactccg cacggccagt
       61 tgtaatgctt cccactcgcc ctttgcaaca gacacctgga tcgactgcct ttcacggacg
      121 ggcgggtcct ctcgaaaaac cttgacgaca gcagggacct gccacacgac aaaagcgtcc
      181 tttgccatag gtgcggcttc tcgcttaaca cacgatacat tgcacacctc ggccacgaag
      241 atggcatcgt gccaaagggt tcccgacacg ttggtggtga gatgaagcgt aaggcttgct
      301 gcatcgttgg gagtcttgac cagaccgctg acaagggtcc atggctgtgt ggcacgaact
      361 cccggcccaa cacttgtcat tccacctgat ttgcacaatc gcccctggct atcatggaag
      421 tgaatgtgaa tacgcgcctc gccttccaag tcttgacact taagccaacc ggccacaagg
      481 taagttgtcc cggcctttac agggattctt tgctgccagc ctcgccaagc tgagggagtt
      541 cccgaaggta tgtccagtct ggccgcgcgc cgtccaagac cctcatgttt cggatcttcc
      601 acagaaaatt tcacgcgacc gtcccctgcc ccggattgga cccagccctc tgggatatca
      661 ccgccagttt caaaggaaga gttctgcacg aggttgttac tgctattaag cagggccata
      721 tattcaagga gcactttgtt agtagcgtcc ttttcctcga tgggctgcac tgatatgggt
      781 tgcactgatg tagggtcctc acagtaaaca tagcgatgga ctcgagatct tgccggtaaa
      841 tgatcaggcc acaggaggta ttccccaaca acgaaaggct taatgcgtgt ggtccccgca
      901 aacatgcgaa ccctatctgt ttcggctaac agcggtaatc ggccacccca aacgttgggc
      961 ccaaggcacg cgagcacgtt ttgcctggct tgcgtgtcaa aattgagaag ctccaaatgt
     1021 agtctctgat ttcccaaaaa gtgattttct tgggacgacg gccaagcttg cgtctcgtcc
     1081 acctcggcca gcgctgacga ttctggcgaa tggatgacga cctcgcaggc ctgtgggtcc
     1141 atttcccact cgacgtcgtc gaaccaagcg cggcctgtgc cccaaagggc cgtcccgaga
     1201 ctggcggtgg ttgccccctc gggcgctgtg aattcggctt ctacttgttt ccaatcgtaa
     1261 gttccaccac cgccggagat ggtcggggca atcatcatgg ggttttctgc atttcccaca
     1321 tggatgtacc aaccggcgta tccctcaacg ttttctgccc gtacccatgc ccgaaagcgg
     1381 tatcgaaccc ctggcaccac ggcaacaccg gtctgtcgag tagcaatcca gcttggtttt
     1441 gatcccggct ggacttcaat tgccagacat tttgccccgg agtgcggatt ctcgttgctc
     1501 cagtaggccc gtcggtccgg accgctggca tcgtgctgcc atcccgaggg tgtttcccct
     1561 tcgccctttt ctacgccaca atttatcggg cacggcttgg ccgagaagta gtcaggtacc
     1621 tcgatggcct cggggttgtc aaaatagacg aagaattccg tctttcctct tgccggacat
     1681 gtcactggca caacgagcca tcctttgggc ggaaatgttc cctcggtgaa agacaccata
     1741 tcttcgtcgc aggccgccca aagtaattcg gttccgtcag ccgtgcagac gcgaatatcg
     1801 cggatattgc gtcccgcaag gggaatccca gaagctgcac ctgccgaaag actaccagga
     1861 catacgacgg gtagcgcaac ggggtatcca gccaaatccc tggaccaatg attgtgatag
     1921 gtgacccgaa cacggctctt ccaatacccg ccccaatgga gataaagcgg atggtgccaa
     1981 ctgacccccg gtgcattctc cttttgggtg tcgtgcatcg cttccaccgc aaaagtggat
     2041 cgtagccgca cggtgctcaa gctactagcc gccagaagca caagacctat caaggtagtg
     2101 acgttcttga ggcgattcat cgacaataac tccctggttg gagcctctct gggggacact
     2161 attgcgaggc actcggcaaa tgtggtgttc attctacggt aataaggcag tcaacgatag
     2221 agagtgccca gaatcgaaga aaccggtggc gtggaggccc tagtaagatg atgtttcaaa
     2281 gagaagtgac agccggtcaa tcatccaaag tcgcgatcaa gcgcacgaag atactcaatc
     2341 gcgtcttcca ggtctgggac agcgactcct acctgtcgcc ccgcacgatc acacctggcc
     2401 aataagacga gtaaatcata atcctctgag ctttccagtc gccgacgggc acgctggcca
     2461 atactgccat ccaaaacttg cagcccctcc atgtggtggg caatcagcca ggccgtgcgc
     2521 tctgttatgt atccgtctag agcctccaaa gcggccgcga cgtgatcttt tgggtcaatc
     2581 gcctttccca catcgtgaag aagagccgcc aaaagaaact cctcatcgta gggcgattcc
     2641 gatcgagcta agtgaaacac ctgaagactg tgtgttagga ggtcgatgtc tggctgaccc
     2701 ttacggtcgt ctcgaatgtg ctcaaggggc accaggagtg actcataagc caaaaaacga
     2761 tcgaccaggg ccggttcctc ctgaggacac gtacgataaa cgagggcggg atgttctcgg
     2821 gcaataagac gttccagctc cccaatattc gctcgttgaa tcggctgccc ggtcgtcaca
     2881 ttgatcggta gttgaccgac catcgccgcc ggatacagag ttagccgaca gacaaaccgg
     2941 gatgtgacgt gaaaacaaac acaacttttc tcggagcctg ctgtttcacc tggtctgttg
     3001 acaatctcaa acgggatgtg cgacttttgt agcgtgtcct gaaccgcctc aacgctgtcg
     3061 gagaaaatgt ggatgtcgat cggcgagcct cgacgagcat gaccggtgag cgtaccgccg
     3121 acaagataag gctggtactc tttcagaatc tgcatgattc ggagtgcctc gagccgcatt
     3181 tcgccgacct caacaccccg gcgttccccc tcaaaggctc gcgccacagc ctcgatctgt
     3241 tcccggatct cacgatttgt cggtaagtcc cccagaggcg cctcgccgcg acaaatccgt
     3301 ctggccgcct tcgtcctggc gcgttggtat tccgtttcct cacgaaaata tataagccgt
     3361 gccgcttccc atgcgatgcg acgtctcaat ttgtcttggg acatgctacg atcgcgatcg
     3421 gccaatcacg taacgtgaag acaaatctct tcttcgcttc atggcgaaga aggcctggtt
     3481 aaccccgact gctcgtataa cccttttttt ccgattgtac tctccttcaa cgcccgcgtc
     3541 gcaccaaccc cgccgacccg cgcgatcagg tacatcaatc aactgccgtt tctcaaaaga
     3601 ctcgattggc gtaaattaag aaaactggat aagctcggca atccagatgt caaaagccag
     3661 ttcgtcaaaa caatacgcgg gcacggtcaa ccgttaccgg atagcaacgc tttacggggc
     3721 caataccaac gtcgaagaat tctcttgctg ccgttagtcc ttcgtctggg tagtcaagta
     3781 catgggcagg cccatctgtt cgatctgggc cagttgttct tcgatccagt tgatatgatc
     3841 gtcctcgtcc ttggcaatcg attcgagcaa atcccgagtt accgagtcgt tcaagtctat
     3901 agccaattgg atggccttgt tgtacgcctg gatggccccc atctcagcat tgagatcgga
     3961 ttgaagctgc gtttgcacat ctggccccac atggacgtca ccgacctgag atacaatcgg
     4021 ggtgccctca aggaaaat
//
LOCUS       DAOPTR010000243         4034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_17893_length_4034_cov_3.108067, whole genome shotgun sequence.
ACCESSION   DAOPTR010000243 DAOPTR010000000
VERSION     DAOPTR010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17893_length_4034_cov_3.108067"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PKI05_05865"
     CDS             <1..265
                     /locus_tag="PKI05_05865"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOA51764.1"
                     /translation="GAVMDVTDPEPLPPGHPLWTTANVIITPHVGGQSATRADDVVRI
                     MCENLRRWHEGRPLVNLITDKRLGFPKRDGSTPLWIDICDQIP"
     gene            602..1600
                     /locus_tag="PKI05_05870"
     CDS             602..1600
                     /locus_tag="PKI05_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOA51765.1"
                     /translation="MGPLLFEPYFRPQIWGGRRLANVLGKLLPPNGRYGESWEVSAHP
                     LHVSRLRGQSSSESTLASLWEKSKMSLWGESYPSPVEFPWLVKFLDCDDFLSVQVHPD
                     SQLAAELVPTERGKDEVWVIVSADAGAKVYLGLRPDATPSRLRQAIQRGHPEDCLNVY
                     SPQPGDIFHIPPGTVHSAGGGVLIAEIQTSSDATFRLYDWNRLDERGQPRPLHIEQAF
                     RAIHWQREVRAPLRTEFQPRGPGWLSQLAASTANFHIECWQIAARTQIEPPHLGMTVA
                     MVLSGSVILRVNSSPPLTLPKGETVLIPACVNSVLWSNPLDLPAWLVVCSPPVQPR"
     gene            1771..3264
                     /locus_tag="PKI05_05875"
     CDS             1771..3264
                     /locus_tag="PKI05_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51766.1"
                     /translation="MIDQSSRIQPLSLLIFAVTLMFTATLGLAWPNFAQEYQVLGHLV
                     GRTGPGEDYPVAAELRGSTRASKVAEEGNGWVALRLDDGRVVWVTAEDLRSDAALLAS
                     IPPLRPPLTAGPTAAETNGKKPVQTGAPLSSTGGDQIPQTGGPSAAPQSVIDANWRPV
                     SAGASKMGVSPPTAFESKTLPQTQQVSRADPPTSPAAQNPDDLLDQAELELSTILTKD
                     PTQWRFDSIQDKVASLAASRLTPAQIARMDALAAKLATARQIADLSLKAHAYSQPMPG
                     PVVLPYSAAGQPPGSPVRHPTGNLGTSPPGTFSTNALTVVPPPSTRITPESRMAGTRG
                     NTVAIATNQPSVFPPSTPSDLGGIGTTAVFPASFEAPSTGELPQSATPSRPAEMSPEA
                     APGTSRPGPGLSLREEIARELNELRRERFDAVGRLVKAQVRRPSDPPYLVVDESGAIV
                     CYVKPAPGTLLRTYVGHWVGISGHKARLPDGRGLLITAESLRLLDPP"
     gene            3646..>4034
                     /locus_tag="PKI05_05880"
     CDS             3646..>4034
                     /locus_tag="PKI05_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOA51767.1"
                     /translation="MGETAKLILGGQEYELPIRIGTEGNHVIDISKLYQMTGHITLDE
                     GFANTGGTTSSITYIDGELGILRYRGYRIEELAERCDFLEVAYLLIYGELPTAEQLEE
                     FRDNIRQHTLLHEGMKQIFAGFPPDAHP"
BASE COUNT          853 a         1220 c         1091 g          870 t
ORIGIN      
        1 aggtgccgtt atggatgtga ctgaccccga accactcccc ccaggccacc cactttggac
       61 aaccgccaac gtcattatca cgccccatgt tggggggcag tccgccacgc gggccgatga
      121 tgtcgtccgt atcatgtgcg aaaatctccg gcgctggcac gaggggcgcc ccctcgtcaa
      181 cttgatcacg gataaacgct tgggatttcc caagcgcgat ggttcaacgc ctttgtggat
      241 tgacatttgc gaccaaattc cctaaaagcc catcccataa tcttccctct cccgttcagc
      301 gggagagggg tcgcaggtga gggtggctcg aatcgcagcc ggttttctcg atccactccg
      361 acggccgctt accctcactt tagccctccc ccggagggag aggggacttt tttgggatag
      421 gctctaggta ggcttcggca acttgcccag tcatcgcagg cagcggcaaa gacaagtttt
      481 gacaagtgcc tcaggcctag ggaaactggt agaaccagtt cgcgttttgt gggaagatgg
      541 ggctgctttt cccgacgacc aaagctggaa aagcaagctc aatttggtgg gaatcatcgg
      601 gatgggacca ctccttttcg agccatattt tcgtccacaa atctggggtg ggcgccggct
      661 tgcgaacgtt ttgggtaaac tgctcccccc gaacggacgt tacggtgaat cctgggaggt
      721 gagtgcccac cctcttcacg tcagccgatt gcggggtcag agcagctccg aatcaactct
      781 cgcatcactc tgggaaaaat ctaaaatgtc tctctggggg gaatcatacc ccagtcccgt
      841 ggagttcccc tggttggtca agtttctcga ctgtgatgat ttcctttctg tccaggtcca
      901 ccccgatagc caactcgcag cggagttagt tcctaccgaa cgcggcaaag atgaagtatg
      961 ggttatcgtc tcggctgatg cgggggccaa agtctatctg ggtcttcgcc ccgatgcgac
     1021 accgtcacgc ctgcgccaag ccatccagcg cggtcatccg gaagattgtc taaacgtcta
     1081 ttcaccgcaa ccgggtgaca tctttcacat tcccccgggg acagtccatt ccgcaggcgg
     1141 gggggttctc atcgctgaaa tccaaacctc tagtgacgcc acttttcggc tttatgactg
     1201 gaatcggctc gatgaacgtg gccaaccccg accattgcac attgagcagg cttttcgtgc
     1261 catccattgg cagcgagagg tgcgtgcccc gctgcgtacg gaattccagc cccgtggacc
     1321 agggtggctt tcccagcttg ctgcaagcac cgccaatttc cacattgaat gttggcaaat
     1381 cgcggcgagg acacagattg agccccccca cttgggaatg acggtcgcca tggtactctc
     1441 aggctccgtc atactccgcg tgaatagctc acccccgctc actctgccaa agggtgaaac
     1501 agtcctcatc cctgcttgcg tcaactcggt tctatggtcc aacccgctgg acctaccggc
     1561 atggctcgtc gtgtgttctc ctccggtaca accccgatga ggtaagcggc cagcgtcttc
     1621 acttggaggt ggttgaccca ctcttttgac cttgcctacc ctgagttggg acgctataag
     1681 gcttatccga tgtgggaata cccgcccgcg gtgctccgcg caaatgttgc tttttgagaa
     1741 agcgtgcctt atccaagagg agtggcactc gtgatcgatc aatccagtcg gatacagcca
     1801 ttatcactgc tgatctttgc agtgacgttg atgttcaccg caactttggg actggcctgg
     1861 cccaactttg ctcaggagta tcaggtcctt ggccaccttg ttggtcgaac tggccccggt
     1921 gaagattacc cggttgccgc ggagttgcgg gggtcaaccc gggcgagcaa ggtggccgaa
     1981 gagggtaacg gttgggtcgc cctccgcctt gacgacggac gtgttgtgtg ggtcaccgca
     2041 gaagatctcc gtagcgacgc ggcacttttg gcatccatcc cacccctgcg cccgccccta
     2101 acggcgggac ccaccgctgc ggaaaccaac ggcaagaagc ccgtccaaac cggggcccct
     2161 ctctcatcga ccggtggtga ccagatacct caaacagggg gaccttcggc agcgccgcaa
     2221 tcggtcattg acgcaaattg gcgacccgtc agtgccgggg cttctaaaat gggagtttcc
     2281 cccccaacgg ccttcgaatc aaagactttg ccacaaaccc agcaggtcag ccgtgctgat
     2341 ccaccaacgt cgccagccgc tcaaaaccca gatgaccttt tggatcaggc ggaacttgag
     2401 ttgtcaacca tcttgacgaa agacccaacc caatggcgtt ttgattccat ccaagacaag
     2461 gtggcttcgc ttgcggcaag tcgtttgacc ccggcccaaa tcgcgcgaat ggatgcccta
     2521 gcggccaaat tggccacagc caggcaaatt gcggacttat cactcaaggc tcacgcctac
     2581 agccagccca tgccggggcc tgttgtacta ccgtactcag ccgccggcca acccccaggc
     2641 tcacccgtgc ggcatccgac aggtaacctc ggaacgtcac cccctggaac gttctccact
     2701 aacgcattga cggttgtccc cccaccatcc accagaatca cgccggaaag ccgaatggcg
     2761 ggcacccggg gcaataccgt ggctattgct acaaatcagc cctccgtatt cccaccttct
     2821 acgcccagcg acttgggagg gataggtacc accgcggtgt ttcccgcctc gtttgaagcg
     2881 ccttccactg gtgaattacc gcagtccgcc accccctcgc gtcccgcgga gatgtcacca
     2941 gaagcagccc cgggcacatc ccgtccgggc cccggcctat cgcttcgaga agagattgct
     3001 cgcgagctca acgagctccg cagggaacgt ttcgatgccg tgggacgatt ggttaaggcc
     3061 caagtccggc gaccaagcga cccaccatat ctggtagttg atgaatcggg agccatcgtt
     3121 tgctacgtta agccagcccc gggaacgctt ttgcgcacat acgtcggcca ttgggtcggg
     3181 ataagcggac acaaggcccg tttgcccgat ggtcgtggac tactcattac agccgagtcc
     3241 cttcgtctcc ttgatccacc gtagggaggt agaatccaac aggaagggta cgatcccggc
     3301 tgtccaaatc gagtgcgttt ttccccgctc ttcggttaat tgtgtcagcc acttgtcttt
     3361 ccgcatgtcc gacggctggt catttaacgc tctcgagcta cggggatgat gaaagtgatc
     3421 acgacctgcc gcggtatctc gggtccacca acatagggaa agaatcggca aaatggccga
     3481 tttctggcgt tttgggcctg cgtgagtctg taatagcgga aaccattgcc gaacccctca
     3541 gtttttccgt tgcatcatag cgagtagaat aagaggtttt gggacatacg ttaatggtgg
     3601 tacctgtagc ctgaacctat ttgttgcgcc acacttggag aaacaatggg cgagactgca
     3661 aagctgattc tcggcggaca agagtacgag cttcccattc gcattggaac ggagggaaat
     3721 cacgttattg atatctcgaa actctaccag atgacgggcc acatcacgtt ggatgaaggc
     3781 ttcgctaata ctgggggaac aacgagcagc atcacttata tcgacggcga actcggtatc
     3841 ctccgctacc gaggataccg aattgaggag ctggccgagc ggtgcgactt tctcgaagtc
     3901 gcgtacttac taatttacgg cgaactgccg acggcggagc agttggaaga gttccgtgac
     3961 aatattcggc agcacaccct ccttcacgag ggaatgaaac agatctttgc cggcttcccc
     4021 ccggacgccc atcc
//
LOCUS       DAOPTR010000244         4025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_17947_length_4025_cov_3.396725, whole genome shotgun sequence.
ACCESSION   DAOPTR010000244 DAOPTR010000000
VERSION     DAOPTR010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17947_length_4025_cov_3.396725"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..>384)
                     /locus_tag="PKI05_05885"
     CDS             complement(7..>384)
                     /locus_tag="PKI05_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HOA51768.1"
                     /translation="WYKTLNKPSWNPPSQVFGPVWTTLYILMGVAAWLVWRSADARST
                     WLPLALFGIQLALNAAWSWIFFKMQRPDWAFVEIVILWLAIVATLVGFFRHSQLAGWL
                     LVPYFGWVSFAAILNFTIWRLNT"
     assembly_gap    385..394
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(828..2162)
                     /locus_tag="PKI05_05890"
     CDS             complement(828..2162)
                     /locus_tag="PKI05_05890"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HOA51769.1"
                     /translation="MISGDGLMYLRVLPEVESVITAAELTLHTVKDLAEMAKKKGISG
                     WRSMRKQELIEALLNVARREARQRKAKQKSVTSKTSKGGRIKGQSSSDNGKVKRGQPA
                     DSSKASSSAKSVDQKSSRTRSNSASLATSEVSGGTRRKVKAAPPSPEVQKQVEELRRE
                     LIDAKDLANSSREGSLGATKDRLVLMVRDPYWLQAYWEIARQTVERARAALGQNWHLA
                     RPVLRLWEIRRDGTSHVERIHLRDIEIHGRVNNWYINVDHPPRSFEVDIGYVSPEGKF
                     FCLTRSNIVTTPLASAAGPDLDWAILADEYERVYALSGGYDEATDTSELREVLEDRLR
                     RPLGGSVIQRLGMGMEGQTEHRSRFPFHVDAELIVFGHIQAGYQVTIRGEPVPVGPDG
                     RFSLRLPLPDRRHVLPVVGQSPDGAEQRTIVLAVERNTKVMEPLLKENDPQA"
     gene            2756..3061
                     /locus_tag="PKI05_05895"
     CDS             2756..3061
                     /locus_tag="PKI05_05895"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOA51770.1"
                     /translation="MGHRQKRSTKDTRATWVMGYQISSQIITIALELVLPSLMGFFAD
                     RKWHTFPALTLVGVLIGFSAGTLHFVQFAKGLNHSRAKAAKNEQSRQFGEPKEPPHE"
     gene            3079..3591
                     /locus_tag="PKI05_05900"
     CDS             3079..3591
                     /locus_tag="PKI05_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51771.1"
                     /translation="MSGHQVKQINPPFLSTSCVDFARGTVVLAGVVGVISLIFAAVIF
                     LALRNPSGELLRLVLAVVVSGFGGMAGLAVLCFSRGTTWELWAPLAATPVRTAATLLS
                     LALLLVLPAGLVTTSFLLYLIVFYISILGCETILALQQLRKNTASIGSAVRGDGAKRR
                     CGMINHSESR"
     gene            3624..>4025
                     /locus_tag="PKI05_05905"
     CDS             3624..>4025
                     /locus_tag="PKI05_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51772.1"
                     /translation="MGLDIAVAGEHVKDATAFHLPFGIHLDLPLIDLGTIQVPGGTFP
                     FSLFGAGREVIEIPLRIQLTKFMVIELAVAVLMFLIFIPMAWHLRGGRPPRGRLMTFF
                     DAILCYIRNHVARPAIGEHEAGRFLPFLWNVF"
BASE COUNT          892 a         1130 c         1054 g          939 t
ORIGIN      
        1 acccgttcac gtgttcagcc gccagattgt gaagttcaaa atggcagcaa agctgaccca
       61 accgaagtag ggcaccagaa gccatccggc aagctgcgaa tgccgaaaga agccgacaag
      121 cgtcgccaca atggcaagcc agagaatcac aatttccacg aacgcccaat ctggtcgctg
      181 catcttgaaa aaaatccaag accaggcggc gttcagggca agttgaatcc caaacagcgc
      241 gagcggcagc catgtggacc gcgcgtccgc agacctccat accaaccaag cggcaacgcc
      301 catcaagata tagagcgttg tccaaaccgg gccaaaaacc tgactgggcg gattccagga
      361 cggtttgttc agcgttttgt accannnnnn nnnnccactt gggaagtcgt ggctatcgac
      421 cccaacaagc ctgctcccag gcataccccc acgattaaaa caagcccaat cacttgctta
      481 gctgatccca tcatgattcc ttttgtgagg caagagtttc cttttgatcc cgcaataggc
      541 ccctgttttg gggcaatccc tgtaatttgt catttgttcc gtaccaggta ttttatgggg
      601 aaatacgcgc ccggacgcgg tgtgaagcca agaaaccctc cccagttgcc ctccagagag
      661 gaaatgacaa agcctcgatg atcgcgcgaa gagcaatcag aaatgcagga ataggattcc
      721 caccaacacc tttttcgctg aaagacaaaa acgatgcggc aaggatcagg gcaaggcttg
      781 ggaatacaca gggggcacac taagacacaa ccgatcaccg ggagcgctca tgcctgggga
      841 tcattttctt tcaacaaagg ttccatcact ttggtgttac gctccacagc gagaacgatt
      901 gtccgttgtt cggccccatc agggctttgc cccacaacgg gcaacacgtg tcggcgatca
      961 ggcaacggca atcgcaggga aaaccggcca tccggcccca caggcacggg ttcaccccga
     1021 attgtcacct gataacctgc ctggatgtgc ccaaaaacga tcaactcggc atcgacatga
     1081 aaaggaaacc gagagcgatg ctccgtttgc ccctccatcc ccatgcccaa ccgttgaata
     1141 accgatccgc ccaagggccg ccgcagccgg tcctccaaca cctcacgcag ttcacttgtg
     1201 tcggtggctt catcgtatcc gccactgagg gcatataccc gctcgtactc atccgcgagg
     1261 atcgcccagt caagatcagg cccggcggct gatgccagcg gtgtcgtcac aatgttactt
     1321 cgggtgagac agaagaactt accctcgggg ctcacgtagc cgatatcgac ttcaaaagac
     1381 cgcggcggat ggtcaacatt gatataccag ttattcacac ggccgtggat ttcgatgtca
     1441 cgaagatgga ttcgctccac atgactggta ccatcacggc ggatctccca cagccgcagg
     1501 accggccgcg ccaaatgcca attctgcccc agcgccgcgc gggcccgttc caccgtttga
     1561 cgagcgatct cccaataggc ctgcaaccag taggggtcgc ggaccatgag aaccaaacgg
     1621 tctttggtcg cccccaagct accttcgcgg gaactgttcg cgaggtcttt tgcgtcaatg
     1681 agttcgcgcc gtaattcctc gacttgtttt tgcacttccg ggctaggcgg tgcagccttg
     1741 acttttcgac gagttccacc cgagacttcg gacgtggcga gagaggcact atttgatcgc
     1801 gttcgagacg atttttggtc aacggacttc gctgaagaag aagctttcga cgagtctgcc
     1861 ggttgcccgc gttttacctt cccattgtca ctactacttt gtcccttgat cctgccccct
     1921 ttactcgttt tactggttac cgatttctgt ttggcttttc gctgcctagc ctccctcctt
     1981 gctacgttta ggagggcttc aataagctcc tgtttcctca tggagcgcca accggaaatt
     2041 ccttttttct tggccatctc ggccaagtct ttcacggtgt gtagtgtcaa ttccgcggcg
     2101 gtgatcacag actcaacctc cggcaaaacg cgcaaataca tcaggccatc gcccgatatc
     2161 acattccctt acgctccgtg tcatcgcatc gccgctaggt cttgacgtct ggccggctgg
     2221 tgggccaaaa catacgcagc catcgaggac acgagacgct ggtctaggag gtcatccgtg
     2281 ccctcattct cagtcgcacc acaggggaag gttctgcccg aaataaaggt gactcgatac
     2341 ttagcagaca gcgccggcga tcgctcgtcc ccagggaccg caccgcttgt tgtcgctccc
     2401 tgctatggct atgctactga ggtttattgt acactgtgcg cctatctttc gcaaccgtgg
     2461 cgccattggg agtattttca ttgggccagc gatcacccaa aaggcctctt gcattttcgc
     2521 ttcagcgagg tttttcgcat catcacaaaa gatacatatt aattgacgaa taggagctta
     2581 atagagttgt cttaatgtac cgggctaacc cactgaggtg ggttatttgc gcatctaccg
     2641 aacgattggc gcgagcaata tagctgaact gggatatttg gagagaagaa ggcaaaatta
     2701 atcggtagga gcgatttccc ctgcgtcatt ccgtcggcag aagcgggaga gttcgttggg
     2761 acatcgacaa aaacgttcca caaaggacac gcgggcaacc tgggtgatgg gttaccaaat
     2821 aagctctcag attatcacca tcgccttgga actcgtctta cccagcctga tgggtttttt
     2881 cgcagatcga aagtggcata cctttccggc gcttacattg gtgggggttc tcatcggttt
     2941 ttcggcgggg acgctacatt ttgtgcagtt tgccaaagga ttgaaccact ctcgcgcgaa
     3001 ggcggccaaa aacgaacagt ccaggcaatt cggagagcct aaggaaccgc cccacgaata
     3061 gcataatggg taacctccat gtcgggtcac caggtcaagc aaatcaatcc gccctttttg
     3121 agcacatcct gtgtggactt tgcgcggggt accgttgtgc tagccggggt ggtgggggta
     3181 atttcactga tttttgcggc cgtgattttt cttgctctga ggaacccaag tggcgaactc
     3241 ttgcggctag tgcttgcggt agtggtcagc ggctttggcg gtatggccgg tctggccgtt
     3301 ctttgtttca gccgaggtac gacctgggag ctttgggcac ctctcgcggc cacgcctgtt
     3361 cggaccgcgg ctacactgct atctctggcg cttctgctcg tcctgccagc aggactagtc
     3421 accaccagtt ttctgctgta tcttatagtg ttttacatct cgattctggg atgcgagacg
     3481 attctagcgc tacagcagct cagaaagaac actgcttcga taggctcggc cgtacgtggc
     3541 gacggtgcga aacgccgatg tggaatgatc aaccattcgg agtcccgcta gcgaggtaag
     3601 ctctaagtgg aggccggcga aagatgggac tggatatcgc ggtcgctggt gaacacgtca
     3661 aggatgcgac ggcttttcat ctgccctttg gcatccactt ggacttgccg ttgatcgact
     3721 tggggacaat ccaagttcct ggtggtacat ttcccttcag ccttttcggg gcaggtcgcg
     3781 aagtcatcga aattccgcta cggattcaac tcacgaagtt catggtcatc gagttggctg
     3841 tggcggttct catgtttctg atctttattc cgatggcatg gcacctgcga ggaggacgac
     3901 cgccgcgcgg gcgactgatg actttttttg acgcaatcct ttgttatata cgcaatcatg
     3961 tggcgaggcc cgccattggc gagcacgagg ccggccgttt tctgccattt ttgtggaatg
     4021 ttttc
//
LOCUS       DAOPTR010000245         4021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_17970_length_4021_cov_3.612204, whole genome shotgun sequence.
ACCESSION   DAOPTR010000245 DAOPTR010000000
VERSION     DAOPTR010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17970_length_4021_cov_3.612204"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /gene="metK"
                     /locus_tag="PKI05_05910"
     CDS             complement(<1..792)
                     /gene="metK"
                     /locus_tag="PKI05_05910"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOA51773.1"
                     /translation="MVNGRFLFTSESVSMGHPDKLADQISDGILDALIAEDPTSRVAC
                     ETLVATGMAIVAGEITTKAAINYPDVVRQVIRDVGYTSDSMGFNYETCAVLVSLRRQS
                     PDIAMGVNADTARGKPIGAGDQGMMFGYAINHTPELMPLPISLAHRILQRLTEIRQRG
                     EVGWLRPDSKSQVTVEFDGPVPVRVDTVVVSTQHSDDITQKEIRDFVIEKVVNPILPP
                     DLVNGEIKYYVNPTGRFVTGGPHGDTGVTGRKIIVDTYGGWAKHGG"
     gene            1176..2234
                     /locus_tag="PKI05_05915"
     CDS             1176..2234
                     /locus_tag="PKI05_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOA51774.1"
                     /translation="MVSAAKSSEQGSYTFSRRNMLLASGAVGLGLVAGKSPAFSALLA
                     EGAQGTAQWPVTCRDVMLKRTGEPDCWAAMAAYNVDGVESDITDDLRLPSLYQGDRVF
                     SVAEPQSRQELKAALEAAKKRITAFCMHNRFEERPEFELEWCKKVAVAAKELGVPAIR
                     IDVVPRRMPRTEFLPFAVNLLRKIVDATEDTGVKFAVENHGNTTNDPTFLDALFEGVG
                     SPRLGLTLDTGNFYWFGHPLSRLYEIYERFAHRAYHTHCKSIQYPADMRDKERPMGWE
                     YQKYHCPIDQGDIDFAKVIGILRKAGYSNDLCIENEGLGRLSAEEAVQTVRKEAQLLR
                     NLQSAITQEGRFMPVARA"
     gene            2411..3064
                     /locus_tag="PKI05_05920"
     CDS             2411..3064
                     /locus_tag="PKI05_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51775.1"
                     /translation="MWCNRYQQDIPAFRGPDGQWVCLRCGSGSRSTETGATEAEHLIA
                     QVGSSSSFETPSVSPNEKTESDTPRYDAWELDEELAHLKRLLSIHQVKPGELVTRFDA
                     YHALAKPAHLRKSQVHHDLLRAERLAEAEHGPAWWLFLCSVTIFGCGAILLGWGLALN
                     HERLLIWGIPIFVAGLLVLIASILLPILERRSSRLSPHLPMKRVKTNMETLQAGRDV"
     gene            complement(3101..>4021)
                     /locus_tag="PKI05_05925"
     CDS             complement(3101..>4021)
                     /locus_tag="PKI05_05925"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOA51776.1"
                     /translation="PHDRVSSGSAGGYFDRHKLRPCGPSVHISQTTVIVSGVGGGVIS
                     YQDVVWDTGYPGVPPGYCYYRVPVAYCYPRTVPVYYYQPRLIYFDPAAPLLRDLAAVT
                     NRYIGTTRPQPSSSGVAQGSTATAQADHVNENRGPSGLAVAEPIKFRPSNEQQRALAS
                     RFLTFGDQQFSAGQYRDAYFKYKEAEKAAPDVAESLFRQGFALIATKQYELAFQAFRR
                     GLRIDPTWPKKPFSVQRLYHERELDFREHLANLAAECEKKNHDPMLAFLLGVMLFRSG
                     RAEDAANFFEIARSSPVSADWAALFTNDEP"
BASE COUNT          926 a         1106 c         1094 g          895 t
ORIGIN      
        1 gccgccatgc tttgcccagc cgccataggt gtccacgatg atttttcggc cagtcacacc
       61 tgtatcgcca tgcggcccac cggtgacgaa cctccccgtg gggttgacgt aatacttgat
      121 ttcgccattg accaggtcgg gcggaaggat cggattaacc accttctcga tcacaaaatc
      181 gcgaatctct ttttgggtaa tatcatcact atgttgcgtt gacaccacga cggtatccac
      241 ccgaacgggc accgggccat cgaactcgac ggtcacttga ctcttgctat ccgggcgaag
      301 ccaacccacc tcaccccgct gtcgaatttc cgtcaatcgc tgaaggattc gatgggccag
      361 ggaaatcggc aagggcatca attcgggggt gtggttaatt gcgtacccga acatcattcc
      421 ctgatcgccg gccccgatgg gcttcccgcg agccgtgtcg gcattcacac ccatggcgat
      481 gtcagggctc tgccgccgca gcgagacgag cacagcgcag gtctcatagt taaagcccat
      541 gctgtcgctg gtgtatccga catcacgaat gacctggcga accacatcgg ggtaattgat
      601 ggccgccttc gtggtaatct ctccagccac aatcgccatg cctgtggcaa ccagggtctc
      661 gcaggctacc cggctggtgg gatcttccgc gatgagagca tccaaaatgc catccgaaat
      721 ctgatctgcc aacttgtccg gatggcccat gctgactgac tcacttgtaa acaagaaccg
      781 accattaacc acggagactg cctcctgaat taaaacgcct cagaaagaat cttttcaact
      841 gagtcccagc gacagcttaa accaatgatt ataggttgct cgcccatgcc tcagcaaggt
      901 taggccaaac cggggtcttt taagccttgc ccagcaggca agcggtgagg cagtaaacta
      961 ttgtcataca gggtagtgcc ccatttgaac ggcgattaca gattcttcgg cgctcacgga
     1021 tggacactct acaatgtaac cacccctttg cttgattttc cgatcctttc gagtcagaat
     1081 ttggtgtatc gttcgcgata tcttcgatgt gttggcacgg aggcttgggc caagcgcaat
     1141 tctctccgga gacaaacacc atgaggactt cccaaatggt ttcggcagca aagagctcag
     1201 agcaagggtc gtacactttc agtcgccgca acatgctttt ggcttcaggg gccgtgggat
     1261 tgggcttggt ggccgggaaa agccctgcat tctctgccct tctagcggaa ggggctcaag
     1321 ggacagcgca gtggcccgtc acgtgccgcg atgtcatgct caagaggaca ggcgagcccg
     1381 attgctgggc tgccatggcc gcatacaacg tggatggtgt cgagtcggac attacggacg
     1441 atcttcgctt gccgtcgtta taccaagggg accgcgtttt cagcgttgcc gagccacagt
     1501 cgagacagga actcaaggca gccctcgaag cggctaagaa acgaatcacc gccttctgta
     1561 tgcataatcg ttttgaagaa cggcctgagt ttgaactcga atggtgcaaa aaagtcgctg
     1621 tagcggcaaa ggaattgggg gtcccggcca ttcgcattga tgttgttccc cgtcggatgc
     1681 cgcgaacaga gttccttccc tttgccgtga acctgcttcg aaaaattgtg gatgccacgg
     1741 aggacaccgg cgtgaagttt gccgtcgaaa atcacggcaa taccaccaat gatccaacgt
     1801 ttttggatgc cctgtttgaa ggcgtgggtt caccacggtt gggcttaacg ttggatacgg
     1861 gcaatttcta ttggtttggc catcccctca gtcgccttta cgagatatac gaacggtttg
     1921 cccatcgtgc ataccacacc cattgtaaga gcattcaata tccagccgac atgagagaca
     1981 aggaacgtcc tatgggctgg gaataccaaa agtatcactg ccccatcgac caaggcgaca
     2041 tcgacttcgc caaagtcatt ggaatcctga ggaaggccgg ttacagcaac gacttgtgta
     2101 tcgagaacga agggctggga cgactcagcg cggaagaagc cgttcagacc gttcgcaaag
     2161 aggctcagct cttacggaat cttcagtctg ccatcactca agaagggcgt tttatgccag
     2221 tggcgcgggc gtgagaagtt gctcgccgcc cacaacctgt ttttcctggc cttcccatct
     2281 agcgcgaatt cgcgtttacg ccggatgcgt aaactagtga tgtgcgcttt ttctggtgga
     2341 atggctgttt cctgtagaat cggcaaaata acaaaagttt tccggtccgt ctgtcgatac
     2401 aggaaacgcc atgtggtgta accgttacca gcaagacata ccggcgttca gaggacctga
     2461 cggacagtgg gtttgcctcc gttgcggctc aggaagtcgc tccacggaaa ccggggctac
     2521 ggaggcggaa catttgatcg cccaggttgg ttcttcttcg agtttcgaaa cgccctccgt
     2581 ctcgccgaat gagaaaacgg aatcggacac accacgttat gatgcctggg aattggatga
     2641 ggaacttgcc catctcaaaa ggcttctcag catccatcag gttaaaccag gtgaattggt
     2701 cacccgattt gatgcctatc atgctttggc aaagccagcc cacttacgga aatcgcaagt
     2761 ccatcacgat ttattgcgcg ctgagcgact cgctgaagcc gaacacgggc ccgcgtggtg
     2821 gctctttctt tgtagcgtca cgatcttcgg ctgtggtgct attctccttg ggtgggggct
     2881 ggctttaaac cacgaacgcc tattgatttg gggaatccca atctttgtcg caggactgct
     2941 cgtccttatt gcctcgatct tgttgcccat tttggagcgg cggtcatcgc gtctatcacc
     3001 tcatcttccc atgaaacgcg tgaaaaccaa catggagaca cttcaggcgg ggcgagacgt
     3061 ctgatctcag tttcccacga tggaagacgg caaacccgcg ttatggttcg tcgttagtga
     3121 aaagggccgc ccaatcggca gataccggag acgagcgggc aatctcaaaa aagttagccg
     3181 catcctctgc gcgaccactg cgaaatagca taaccccaag caaaaaagcc agcattgggt
     3241 cgtggttttt cttttcacac tccgctgcca agttagcaag gtgttctcgg aagtcgagtt
     3301 cccgttcgtg atacagacgc tgaaccgaaa atggcttctt cggccaggtg gggtcaattc
     3361 tcaaaccacg gcggaatgcc tgaaaggcca gctcatattg tttcgtagcg attaaagcaa
     3421 atccctgccg gaaaagtgat tccgccacat cgggggcggc cttttccgcc tccttgtatt
     3481 tgaaataggc atcccgatat tgcccggccg aaaactgctg atctccaaac gtcaggaacc
     3541 gactggccag tgcccgttgc tgttcgttgc ttggtcggaa cttgatcggc tccgcgacgg
     3601 cgagccccga cggaccgcgg ttttcgttaa catgatccgc ttgtgcagtc gcggtggaac
     3661 cctgggcgac gcccgaagat gacggttggg gccgtgtggt cccgatatag cggttggtca
     3721 cggccgcgag atcccggagg agcggcgcgg cgggatcgaa gtagatcaat cgcggctggt
     3781 agtagtaaac agggacggtc cgaggatagc aataggctac ggggacccga tagtaacagt
     3841 aaccgggggg aacaccagga tacccagtgt cccaaacaac atcctgatat gagataacgc
     3901 caccgcccac gcccgaaacg ataacagtcg tttgagaaat atgcaccgaa ggcccacacg
     3961 gccggagctt gtgtcgatca aagtaacctc cagcacttcc agacgacacg cggtcgtgag
     4021 g
//
LOCUS       DAOPTR010000246         4000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_18071_length_4000_cov_4.174398, whole genome shotgun sequence.
ACCESSION   DAOPTR010000246 DAOPTR010000000
VERSION     DAOPTR010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18071_length_4000_cov_4.174398"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1985)
                     /locus_tag="PKI05_05930"
     CDS             complement(<1..1985)
                     /locus_tag="PKI05_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51777.1"
                     /translation="MKYFLTILITLVTCWISSPTWGETIWQEGENPASHKMNRHPWWY
                     DQVKKEHLSGGDWISNFSEEKEGTVEYSVSLKDGGDYVLWLRINPVRSRVSLSVDGSP
                     WRELDLSAATQVVNIAADNKIDLRFIGWVNAGRLTLSRGDHTLAFRFHSELQNHGGLD
                     AFVLTNEPFSPSGLARPGTAETRADVVTEIGTWPFEPSVDPIRAGALLDLRSLNEQTA
                     GEKGFIRLSEDGMSFLRGDGQPIRFWAVVDYGWRMEPDVMRRHLRWLAKLGVNMVRVH
                     AQLCVEKEGAKLTDVDEKVIDQIQRYVAECKNHGIYVTISPYWSHTSKIPASWGLDSY
                     TGQSGPEGLIFFEEKLQDAYKTWVKELYTRPNPYTGIPLSQDPTVAIIQVQNEDSILF
                     WTSQNIREPFAGKLRRLFGRFLVEKYGSLEKAQDAWGDARHKQDDFASGEVGMEIIWR
                     MTSQAPKPNAAWAKRLADQLEFMSRLQHQFYADMAKFYRETLGCKQLLNATNWRTADP
                     VLLEDCERWTYTAMDVAALNRYTGVIHVGPMNGWRIDPNHYYISQSCLLNPEEFPGAV
                     KQTVGQPMILTEVAWVHPALYQTEGPFLAAVYMSLTGVDAFYWFSYGDEFWTADPVWP
                     WWKVGGLNSLKKWEGSVPTQAGMFPAAALAYRLSYIRA"
     gene            2351..3316
                     /locus_tag="PKI05_05935"
     CDS             2351..3316
                     /locus_tag="PKI05_05935"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOA51778.1"
                     /translation="MGEARIVSFINYKGGVGKTTTTYHIGCSLAEHHGHRVLLVDIDP
                     QSNLSLLCMSYDRYNQFRTQQGTIRDLYRRFRQESRLGTRDFIVREPIQLNNGPLRNL
                     DLLPCDLDMLGEDIGGILPTVSAARTGNAFQVLEQVAERVLREWDFLRLALDEVRSEY
                     DDILIDCPPNLYLMTQNALAASDWYVITVVPEHLSSVGVPILIRKIRDIRERIRRVAA
                     ILGREVSFARFAGIIAVKMRRAGQHQDVLNRLREDLSSYIGEGSLADPVFATATTELI
                     GYSEAAEQNLPVWHARGNNPAMAAERREYEAITEEYLRRCHSGEL"
     gene            3401..3919
                     /locus_tag="PKI05_05940"
     CDS             3401..3919
                     /locus_tag="PKI05_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51779.1"
                     /translation="MAKRQSASTLEVLEELSGVFNLLPTEEFRNEVTTSVQQIIGFLQ
                     DLRVAIEKIPTREQMIDRRIPESLDRVRDFLKPFESAGRARSSRQAQRSVKKVVDLEE
                     FKDHLMTLPIGQIEGALRDSGLTLEQLKEVLTRLGGSSRGARSKEQVIQRIVDEIRTR
                     RELSGLRDGATP"
BASE COUNT          917 a         1047 c         1106 g          930 t
ORIGIN      
        1 gctcgaatgt agctcaagcg gtaggccagc gctgccgcag gaaacattcc cgcttgagtc
       61 ggtaccgaac cttcccattt cttaagcgag ttcagtcccc cgaccttcca ccacggccag
      121 acgggatcgg ccgtccagaa ttcatcccca tagctaaacc agtaaaaggc atcgaccccc
      181 gtcaaagaca tatacacggc agccaggaac ggcccctctg tctgataaag ggccggatgg
      241 acccaggcca cttcggtcag gatcatcggc tggccgacag tctgtttgac ggcgcccgga
      301 aattcctccg gattgagtaa acacgattgg ctgatgtaat aatggttggg atcgattcgc
      361 cagccgttca tgggccccac atgaatgacg ccggtgtagc ggttcaaggc cgccacgtcc
      421 atggccgtgt aggtccagcg ctcacagtct tcaaggagca cagggtcggc cgtccgccaa
      481 tttgttgcgt tgagaagttg cttgcagccc agcgtctcgc gatagaattt ggccatgtcc
      541 gcgtaaaact gatgttgaag tcggctcatg aactcgagtt gatcggcaag gcgcttggcc
      601 caggcagcgt tgggctttgg ggcctgcgat gtcatccgcc aaatgatttc catgccgact
      661 tcgcctgagg caaaatcgtc ttgtttgtga cgggcatccc cccaagcgtc ttgggccttt
      721 tccagcgaac cgtatttctc gacaaggaat cggccgaaca gtcgccgcag ttttccggcg
      781 aacggctcac ggatattctg tgatgtccag aagaggatac tgtcctcatt ctgcacctgg
      841 ataatagcga ccgtagggtc ttgagaaagg ggaatgcccg tgtaagggtt ggggcgtgtg
      901 tataactctt tcacccaagt cttgtaggca tcttgaagtt tttcctcaaa gaaaatcaag
      961 ccctctggac cggactgccc tgtgtaactg tccagtcccc aactggccgg gattttgctg
     1021 gtgtgagacc aatagggaga aatcgtgaca tagattccgt ggtttttgca ctcggcaacg
     1081 tatcgctgaa tttgatcgat gactttttcg tccacatcgg tgagtttggc cccttccttc
     1141 tccacgcaga gttgggcgtg cacgcggacc atgtttaccc ccaatttcgc tagccaccgc
     1201 aagtgacgac gcattacatc gggttccatg cgccagccat aatccacgac cgcccagaag
     1261 cgaatcggtt ggccgtctcc gcggagaaaa ctcattccgt cttcagaaag ccggatgaat
     1321 cccttttctc cagccgtttg ttcgttcaaa gagcgaagat cgaggagcgc ccccgcgcgg
     1381 attggatcca cactcggttc aaagggccag gttccaatct ctgtaacgac atcggctcga
     1441 gtctccgccg taccgggacg tgccaggccg ctcggtgaaa atggttcatt cgtgagcaca
     1501 aaggcatcta agcccccatg attttgaagt tcactgtgga agcgaaacgc cagcgtgtgg
     1561 tctccgcgag ataacgtcaa ccttcccgca ttaacccaac caataaaacg gaggtcaatt
     1621 ttgttgtccg ccgcaatgtt aacaacttgc gtggccgcag agaggtccaa ctctcgccac
     1681 ggcgaaccat cgacgctcaa agaaacgcga cttctcacag gattgatgcg gagccagagg
     1741 acgtaatcgc cgccatcctt caggctcacc gaatactcca ccgttccctc tttttcttcg
     1801 gaaaaattgc taatccaatc cccgcccgag agatgttcct ttttgacctg atcgtaccac
     1861 caggggtgcc gattcatctt gtggctggca gggttttcac cctcctgcca gatggtttca
     1921 ccccaggtcg gcgaagaaat ccagcaagta accagcgtga ttaggatagt caggaaatac
     1981 ttcatgattt gccgcctcat tccgcaatca tgcattgcaa ggactaccgt agattcacgc
     2041 ggatcttccc tacggcgtta tctgatgtgt gaacgttcgg tgctgcccag ctcaaaggtt
     2101 ctccgtaaag ttgagcaagg ctgagaacat tgcttttccg gggatacctt aattcaatgt
     2161 cggccattgt aaagaacttg aggacctcaa ggaagtacac cgcggaccgc acggctgaaa
     2221 tgcacagttg accgcccaaa aaagccccgt tacgctggtt gcatctaggg gctttggaaa
     2281 gccatcacca ggtgaccaca acgcacgaac gacgttaaag ttcatgctgt ctgattcgag
     2341 gagctgagaa atgggcgagg cgcggatcgt ctcctttatc aactacaaag gcggagtggg
     2401 caagacgacc acgacttatc acatcgggtg tagtttagcc gagcatcatg gtcatcgcgt
     2461 gttgctggtc gatattgatc ctcagtcgaa tctttcgctg ctttgtatga gctatgaccg
     2521 gtacaatcaa tttcggaccc aacagggcac aattcgcgat ctttatcgtc gatttcggca
     2581 ggagagccgc ctgggtacac gggatttcat cgtccgcgag ccgattcagt tgaacaacgg
     2641 tccgctgagg aatctcgacc tgctcccgtg tgatctggat atgctgggcg aagatattgg
     2701 gggaatactg ccgactgttt ctgccgcgcg gacggggaac gcatttcagg tcttggaaca
     2761 ggtggcggag cgcgtgttgc gagaatggga ctttttgcgt ctggcacttg atgaagtccg
     2821 cagcgagtat gacgatatcc tcatcgattg cccgcccaat ctctatttga tgacccagaa
     2881 tgccctggcc gcctctgact ggtatgtcat taccgtggtt ccggaacacc tttcgtcggt
     2941 tggggtgccc atcctcattc gtaagatacg agatatccgc gagcggattc ggcgagtggc
     3001 ggccatcctt gggagagagg tttcgtttgc ccggtttgcc ggtattattg ctgttaaaat
     3061 gcgtcgggca ggccagcatc aggacgtgct caaccggctg cgtgaggatc tatcgagtta
     3121 tattggcgag ggtagtttgg ctgatccagt tttcgctaca gccaccacag aattgatcgg
     3181 ctacagcgag gcagcggagc agaatctccc cgtgtggcat gcgcggggaa acaatccagc
     3241 catggcggcg gaacggcggg aatacgaagc cattaccgaa gaatacctga gacgctgtca
     3301 tagcggtgag ctatgagaca gtggcaatga aataaaagga cgcccttttc cagggcgctt
     3361 cgtaggagtg tttcccaatg tcaatgtgaa aggatggatt atggccaaaa ggcagtcagc
     3421 ttcgacgtta gaggtgcttg aagagcttag tggcgttttt aacttgttac cgactgagga
     3481 gttccggaac gaagtcacta catcggtgca gcagattata gggtttttgc aggatttgcg
     3541 tgttgcgatt gaaaagatcc ccacccggga acagatgata gataggcgta ttcctgagtc
     3601 acttgatcgc gtcagggatt tcttgaagcc cttcgagtcg gccggcagag ccagatcgtc
     3661 gcgtcaagcg cagcggagtg tcaaaaaagt agtcgatctc gaggagttca aagaccatct
     3721 gatgaccctt cccattgggc agattgaagg cgcactgcgg gattcaggat tgacattgga
     3781 gcaactgaaa gaggtgttaa cgcgtctcgg gggttctagt cgtggtgcta ggagtaaaga
     3841 gcaggttatc cagcggatcg tggatgagat tcgtactcgg cgggaactga gtggcttacg
     3901 ggacggggcc accccttaaa accagtaaga tcaatacctg tttgaccagg gaaatatgca
     3961 atcacggtgg ggacatcgca ccattgcgtg tccccaccgt
//
LOCUS       DAOPTR010000247         3998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_18078_length_3998_cov_3.882577, whole genome shotgun sequence.
ACCESSION   DAOPTR010000247 DAOPTR010000000
VERSION     DAOPTR010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18078_length_3998_cov_3.882577"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..2217
                     /locus_tag="PKI05_05945"
     CDS             205..2217
                     /locus_tag="PKI05_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009094241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOA51780.1"
                     /translation="MDLINNDAVVGALFTAANYGESDGSGTVAAASVELSGGVTGGDV
                     IATANDVVAAINSSGASAVVTASLASGDNGYGVVSPFQEYAYYGSVEANNRLQFLGPE
                     GSRKIRFVSNPGEELSVDLTTDPRVEGFASAIVQGVNANSTLEITARYKGEAYDGVQI
                     IFNDSTDNTVVWDAESKTLTFNVDISGGFTAQDAVDLLNNDSYAGKYFRASNFGTSDG
                     SGALSATMTGTTSGGVVSEGTVIVHLATTSDGIVTTTAQDLIDYFNDPANAGLLSGLG
                     ISVSNVGGSDGSGRLAATEADLEFATSGTVLQDAQASGVTYAVNGVNARLQLTAVAPG
                     AEWNGVRLVFEDTATAGNETVSWDAATKTLTVGIESGVSTADQVIAAINNDAVVSKLF
                     TAARATGSNGSGAVTTSDFAQLSGGVQVVGTVEGVPLLGNADQATTGLVFRAVEYGSD
                     AFVSIKALNGTVFEVTDRDGNVTTRNSGTDVNVLVNGIAAVGKGLRASINTAALDLAF
                     TISETLPDGTLTNFRIVGGGAQFQLGPDVVSNQQARLGIQSVNTAKLGGVSGRLFELR
                     SGGPKSLDRDVIGAAAVVEEVISQITTLRGRLGAFQRTTLETNINSLNDTLENLTAAE
                     SAIRDADFAAESAALTRAQILVQSGVSVLAIANQNPQAVLALLRGG"
     gene            complement(2357..2881)
                     /locus_tag="PKI05_05950"
     CDS             complement(2357..2881)
                     /locus_tag="PKI05_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51781.1"
                     /translation="MPNSFCDSPRVPVTCPTPVGSTIWASLFPALLVIVAVLFLAWHF
                     LAWRKVGAKPDLDGAEWNFRRRQFLRRLQVSGMLLILGVLLFVGQFLFSGTEHPGWAI
                     AYWLVVAVITFWMILAAVADMLAGISYFGRLKTQADIERIRLSAIINRFYRIHGNGEE
                     KPKESSAKPEKPPS"
     gene            complement(2930..3208)
                     /locus_tag="PKI05_05955"
     CDS             complement(2930..3208)
                     /locus_tag="PKI05_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOA51782.1"
                     /translation="MAHVVCKPCYGCKYTDCVVVCPVECFYEGEMMVYIHPDECIDCE
                     ACVPECPVEAIYLADNVPEEWKEYIQLNAEMAPQCPKITEKKKPLCES"
     gene            3613..3834
                     /locus_tag="PKI05_05960"
     CDS             3613..3834
                     /locus_tag="PKI05_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51783.1"
                     /translation="MADLNGNQTPTRFLFDPDFCEKRCPICTRARRGVRWARWLRRLE
                     LFLTFGGCPWGRARAKKYGVPPDQPQPAS"
BASE COUNT          873 a          894 c         1338 g          893 t
ORIGIN      
        1 actcggcagt gggaggcggc gagtggggtg ttggatggta cggggacgaa caatgcgata
       61 gagatcacgg cgaatgtgaa gggggcggag tttaatgggg tgacggtgaa tttgacgatt
      121 ggggcggcgc ggagtgtggc gtatgatcac aatacgaaga cattgagtat tacggcgttg
      181 gcgacggata cggcgcagga cattgtggat ttgatcaaca atgatgcggt ggtgggggcg
      241 ttgtttacgg cggcgaatta tggggagtcg gatgggagtg ggacggtagc ggcggcgagt
      301 gtggagttga gtgggggcgt gacggggggc gatgtgattg cgacggcgaa cgatgtggtg
      361 gcggcgatca acagttctgg ggcgagtgcg gtggtgacgg cgagtttagc gagtggggac
      421 aacgggtatg gggtggtgag tccgtttcag gagtatgcgt attatgggag tgtggaggcg
      481 aacaatcggt tacagttttt gggtccggag gggagtcgga agattcggtt tgtgtcgaat
      541 cctggggagg agttgagtgt ggatttgacg acggacccgc gggtggaggg gtttgcgtcg
      601 gcgattgtgc agggggtgaa tgcgaacagt acgttggaga ttacggcgcg gtacaagggg
      661 gaggcgtatg atggggtgca gattatattc aatgacagta cggacaacac ggtggtgtgg
      721 gatgcggaga gcaagacgtt gacgtttaat gtggacatta gtggggggtt tacggcgcag
      781 gatgcggtgg atttgttgaa caatgacagt tatgcgggga agtattttcg ggcgtcgaat
      841 tttgggacga gtgatgggag tggggcattg agtgcgacga tgacggggac gacgagtggg
      901 ggtgtagtga gtgaggggac ggtgatcgtg catttagcga cgacgtcgga cgggattgtg
      961 acgacgacgg cgcaggattt gatagattac tttaatgatc cggcgaatgc gggtttgttg
     1021 agtgggttag ggatcagtgt gagcaatgta gggggtagtg atgggagtgg gcggttagcg
     1081 gcgacggagg cggatttgga gtttgcgaca tctgggacgg tgttgcagga tgcgcaggcg
     1141 agtggggtga cgtatgcggt gaatggggtg aatgcgcggt tgcagttgac ggcggtggcg
     1201 ccgggggcgg agtggaatgg ggtgcggttg gtgtttgagg acacggcgac ggcggggaat
     1261 gagacggtga gttgggatgc ggcgacgaag acgctgacgg tggggattga gagtggggtg
     1321 agcacggcgg accaggtgat tgcggcgatt aacaacgatg cggtggtgag caagttgttt
     1381 acggccgccc gggccaccgg gagtaacggc agtggtgccg tgaccacctc agacttcgct
     1441 caactctccg gcggtgtgca ggtcgtcggt actgtcgagg gtgtcccctt gctaggaaac
     1501 gctgatcagg cgacaactgg actggtgttc cgagcggttg aatacggatc agatgcattt
     1561 gtgtcgatca aagcactcaa cggaacagtc tttgaagtga cagaccgcga tgggaatgtg
     1621 acgactcgca acagcgggac cgatgtcaac gttcttgtta acggtatcgc ggcggtcggg
     1681 aaaggattaa gggcctcgat caatacggct gcgctggacc tggcattcac gatctctgag
     1741 acactccctg atggaaccct cacgaatttc cgtatcgttg gtggtggtgc ccagttccag
     1801 ttggggccgg atgttgtttc caaccaacag gcacggctag gcattcagag cgtgaatacc
     1861 gcgaagctcg gtggggtgtc aggccggctg tttgagcttc gatccggtgg cccaaagtcc
     1921 ctagatcggg atgtgattgg cgctgccgcg gttgtggaag aggtgatttc gcagatcaca
     1981 acccttcgtg gtcggttggg tgccttccag cgaacaaccc tggagaccaa tatcaattcc
     2041 ctcaatgaca ccctggaaaa cctgacggct gccgaaagcg ccatccggga cgccgatttc
     2101 gccgcggaga gtgctgcctt gacacgcgcg cagatccttg tccagtcggg tgtctctgtg
     2161 ctggcgatcg ccaatcagaa cccgcaggcc gtgttggctc tcttgcgggg tggctgattg
     2221 aggaggcggc aagttcgctc cttcgcactg ataaaaaacc gcaatctagg acgcatcgtc
     2281 aacggattgc ggtttttatt ttttggccac atgatagggg cctgttttgg caatagggat
     2341 tgccgcgttt ggcagcctac gacggcggct tttctggttt tgctgaagac tcctttggtt
     2401 tttcctcgcc gttgccgtga attcggtaaa atctgttgat gatggccgaa agacgaatcc
     2461 gttcgatatc ggcctgggtt ttgagtcggc cgaaatacga gattcctgcc agcatgtcgg
     2521 ccaccgccgc caagatcatc caaaaggtga tgaccgctac caccaaccag taagcgatgg
     2581 cccaccctgg gtgctcggtt cccgagaaaa ggaattgccc gacaaacagc agaaccccca
     2641 aaatgaggag catgccgctg acctggaggc ggcggaggaa ttgccgtcgt cgaaagttcc
     2701 attcggcccc atccaggtcc ggcttagctc ccaccttccg ccatgccaga aaatgccacg
     2761 cgagaaacaa aacggccaca atcaccaaaa gtgccggaaa caaactcgcc cagatagtac
     2821 ttcctaccgg ggtgggacac gtaaccggaa ctcttgggct atcacaaaac gaattcggca
     2881 tggtgggtac gttctgcccg ttttacgcag aacgagaatc aagtggctac tagctttcgc
     2941 agagaggttt cttcttctct gtgatcttgg gacactgtgg tgccatttca gcgttgagct
     3001 ggatatactc tttccactcc tcgggcacgt tatccgcaag atagatagct tccacaggac
     3061 attcgggaac acaggcttcg cagtcgatgc attcgtcggg atggatatag accatcattt
     3121 cgccttcgta gaaacattcc accgggcaaa cgaccacgca atctgtgtat ttgcacccgt
     3181 aacatggttt gcaaacgacg tgtgccatac gtcctccttt tcagggttta gtaaaagcgt
     3241 ttgcgagtca ataaattgtt tgccagcttc aaaaattgtc aacgaaatgg gtggcgaaaa
     3301 cgcatctaca ttgagatgcc gacgattaac gcctatcagt tgtctaaact ttcggaactc
     3361 gccccggcct accccacttt gaccccgagg atgatacccc gcctactagt agaaaggaga
     3421 cccggggctt ttcgctatcg gatttttgtc atcgtagagc gacagtattt ctgtgtcaag
     3481 taagcaagcg ccgggaatat gtggccgggg caccacaacc tccacgtgag actttgccca
     3541 gcaattcgct agaattcccg atggtggaag ttatcatcgc acgtcccgcc atttgaagga
     3601 aggcgttaag ccatggctga cttaaacgga aaccaaacgc cgacgcgttt tctgtttgat
     3661 cctgactttt gtgaaaaacg gtgcccaatc tgcacacgcg cacggcgggg tgtccgctgg
     3721 gcacgatggc tgcggaggct cgagcttttc ttgacctttg gcggttgtcc ctgggggcga
     3781 gcgcgggcca agaaatacgg cgtgccacct gaccaacctc agcccgcttc ctaaggatac
     3841 aattcacgga agccatcggt cgatcgccgg tgagcgtgta tttctcaaga cggaaacaac
     3901 aaggggcttc agtggttaag tgagtggagc atgggaagta aacaacgtga cctattccta
     3961 ccaacaacgc ccgaagaagt tgcagccccg ggttggga
//
LOCUS       DAOPTR010000248         3984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_18148_length_3984_cov_3.466276, whole genome shotgun sequence.
ACCESSION   DAOPTR010000248 DAOPTR010000000
VERSION     DAOPTR010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18148_length_3984_cov_3.466276"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2017)
                     /locus_tag="PKI05_05965"
     CDS             complement(<1..2017)
                     /locus_tag="PKI05_05965"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOA51784.1"
                     /translation="MNVPLRSRWSSARRWLTLGVALLVGLLSYHLVFAEEATSAAPAS
                     PSLGGQLSVFVWWIIGLGGSICGLYFAWRFFKEMLNSEKGNERMEEIASYVREGANAY
                     LKQQYTVVACFFVVIVILLAIAAFGLHAQSRWVPFAFLTGGFFSGLAGWLGMKTATLA
                     SSRTAQGCRKSLNDGLRVAFRSGAVMGLTVVGFGLLDICLWFAILYWVISAFGYHMTL
                     EQITVTMLCFGMGASSQALFARVGGGIFTKAADVGADLVGKVEKGIPEDDPRNPAVIA
                     DNVGDNVGDVAGMGADLYESYCGSILATAALGVAAFATGELLPTRILEQVQKGAIALE
                     SAKLNYQLAALFLPIVLAGVGIALSVIGIYAVRSEEDATQRTLLKALGRGINLSTLLI
                     AVATFVFTYLLMPDFWTVAVSVLVGLIAGWLIGKWTEYSTSDEYQPTKFIASQSQTGP
                     ATVIIGGFAEGMKSTWFPVIVVAVATLLAFGFAAQFHFNDLTWFAMGLYGVGIAAVGM
                     LSTLGITLATDAYGPIADNAGGNAEMSHLEPQVRQRTDALDSLGNTTAATGKGFAIGS
                     AALTALALLAAYVEEVRVGFDRWAHSALEQAEPGLYKLGDKFLAEVTGEKGPGGKPII
                     HGYLVFGSAITDPAVAEDWNKLDASREPQKIPEGIIERFEDEVKGIKR"
     gene            3227..>3984
                     /locus_tag="PKI05_05970"
     CDS             3227..>3984
                     /locus_tag="PKI05_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOA51785.1"
                     /translation="MDRRTFLKLSGAVAAQAALWSPQVGRAEAESASAHGLGNPFLQE
                     GRWFKAAFHVHTTTSDGDVDVPTRLNQYRKLGYQVVAITDHWKTNDLSAFAEEDFLPI
                     VGLEMHPQTGTGAPAHHFVVLNVPHPFELDRKLAAQAMIDQVLSVGGKVIYAHPYWTT
                     HSLNEMLEVAGYCALEVFNGGCHVENDTGYGSVHWDQVLNKGRLIGGIATDDVHNSKL
                     INIGWTMIKAPALNAAAIMEAIEKGCYYASAGPT"
BASE COUNT          931 a         1209 c         1038 g          806 t
ORIGIN      
        1 tccgcttgat gcctttcact tcatcctcga accgctcgat gattccctcg gggatttttt
       61 ggggctcgcg agaggcatcc agtttgttcc agtcctcggc gacagcggga tcggtgatgg
      121 cactgccgaa gacgagatag ccatgaataa tcggtttacc gccagggcct ttctcgcccg
      181 tcacttcggc caagaatttg tcgcccagct tataaagccc cggctcggct tgttcaaggg
      241 cagaatgggc ccagcggtcg aaaccaacgc ggacctcttc cacataagcc gccaaaagtg
      301 ccagcgccgt cagggccgcc gaaccgatgg cgaaaccctt tccagtggcc gccgtggtgt
      361 tccccaggct gtcgagggcg tccgttcgct gccggacttg gggttccaag tgggacatct
      421 cggcgttacc accggcattg tcggcaattg gaccgtaagc gtcggtggcc agcgtaatgc
      481 caagtgtgct cagcataccc acggccgcga taccgacacc gtacagcccc atggcaaacc
      541 acgtcaggtc attgaagtgg aactgagccg caaaaccaaa ggcaagaagg gtagccaccg
      601 ccacgacgat gaccgggaac cacgtgctct tcatcccttc cgcaaagcca ccgatgatca
      661 ccgtggccgg acccgtctgc gattggctgg caatgaactt cgtcggctgg tattcatcgc
      721 tggtggaata ctcggtccac ttgccgatga gccaacccgc aatcaaaccg acgagcacgc
      781 tgaccgccac agtccagaaa tcgggcatga gaagataggt aaagacaaac gtggccacgg
      841 caatgagcaa cgtggacaga ttgatcccgc ggcccaaggc cttgagcaat gtcctctggg
      901 tcgcatcttc ctctgaccga acggcataaa taccgatcac ggacagggca atcccaacac
      961 ccgccaggac gatcgggagg aacaaagcgg caagttggta attgagtttg gccgattcga
     1021 gggcaattgc gcctttttga acctgttcca ggattcgagt cgggagcaac tcgccggtag
     1081 cgaaggctgc cacaccaagg gccgccgtgg cgagaatcga accgcagtac gattcgtaca
     1141 ggtcagcacc catacctgcc acgtcgccga cgttgtcacc cacattgtcg gcaatcacgg
     1201 cggggttacg gggatcatct tccggaatac ccttttctac cttacccacc aggtcagccc
     1261 cgacatcggc ggccttcgtg aagataccac cgccaacacg ggcaaagagc gcttggctac
     1321 tggcccccat cccgaagcaa agcatcgtga cggtgatctg ttccagcgtc atgtgatatc
     1381 caaatgccga gatgacccag tagaggatgg caaaccacag gcagatgtcg agaagcccaa
     1441 agccgaccac agtcaggccc atcacggcac cgctgcgaaa cgccacccgc aacccatcat
     1501 tgagcgactt gcggcagccc tgggctgtgc gactactggc gagcgtggcg gtcttcatcc
     1561 ccaaccaacc ggccaagcca gagaagaacc cacccgtcag gaatgcgaag ggcacccacc
     1621 ggctttgggc atgcagacca aatgcggcaa tcgccaacag gatgacaatc accacaaaga
     1681 agcacgccac caccgtgtat tgctgtttca gataggcgtt ggcaccttcc cggacatagc
     1741 tggcgatctc ctccattcgc tcgtttcctt tttcggaatt gagcatttcc ttaaagaacc
     1801 gccaggcaaa atacaaaccg cagattgaac cgcccaaccc gataatccac cacacgaaca
     1861 cactgagctg gccacccagc gagggactag ctggggcagc actcgttgct tcttccgcaa
     1921 acaccaggtg gtaactgagg aggcccacca aaagggcaac tcccaaggtc agccatcgtc
     1981 gggctgaaga ccatcgactc ctcaacggca cattcatacg taacctcgct tgtcacaaat
     2041 cccaaaccgc gtttgaccag tctccgacaa atttcccctg tacaaccaaa ggggcctctt
     2101 ctcggcgatt aacgtctctt ggcaggattc cgcgacgacc gaataactta agtctttcgt
     2161 acgcggacca agacccggcc gttcacccaa aaacagagaa ccaccacctt tggtgatgac
     2221 tcctctgcgt ccctgcgcgt cacaacggtt tccaagccgg cctatttcac agggacatac
     2281 accagagatg ccgaaggcca gggatggtta acgacacgtt ccgcttatgt ttcccccggt
     2341 cccgccctct gcgctcaacg tcgagggctg gacagatcct tcggctgccc aaagtctgga
     2401 cgaccagacg taaacaaagc agtctaacga agctaggggg ttcatgtcaa caatagcatg
     2461 cctttgattg tcggagcccc gcttttcgca tcacccgcaa agtagccacc tattgacaca
     2521 catcccctaa tttacctgaa tgaaatatca attctttctg ataaaaacga ctgaatcggc
     2581 gggactcgct ttctcaacag ccaccaaacg atggggattt ccttaatacc gaagactgca
     2641 tcccatgcct cccgcatttc gagggaggga cgccatcacg tctgacgtcc tgctaaaaac
     2701 ctatccccca agatcccccc tcccgttccg cgggagaagg accagagggt gagcgtggat
     2761 cgaatcgctg ctggttttct cgatggactc cgggggttgc ttaccctcac cccaaccctc
     2821 tcccggaggg agagggaatt tgggatagac tctgattgat gatccattgc cgagcccggc
     2881 tacggcggcc cccggccttc atccacgacg cgtgaggggt gtcccaggcg gacagagaca
     2941 agacctgcct ctacgaatca gtgaaacacg catagatcat caacacacat cccagctcac
     3001 agcggggctt gccatcattt cttgcttctt gcttagtgct catgatgatt ctttccttgc
     3061 ggctcgtcgt ctccgtcacg ggttgggctc aaggtgtctt ccgtgaccat gagagattct
     3121 gtctgctact tgcacgtctt aatctcgctg ccttatattc tggaatatgc caacgtggtt
     3181 tttcggaact cgtttacgcc ctcaacactg ataaggagtt agcatcgtgg atcgacgaac
     3241 ctttctcaaa ttgtccggag cggtggcggc ccaggccgct ttgtggtccc ctcaagtggg
     3301 tcgagccgag gcagaatcgg cgtcggcaca tgggctgggc aatccatttc tccaagaggg
     3361 acgctggttc aaggcagcct tccatgtcca cacgacgacc tccgatggcg atgtggacgt
     3421 tccaacgcgg ctgaatcaat atcgtaagct gggttaccag gtggttgcga tcacggatca
     3481 ctggaaaacg aacgacctca gtgcatttgc tgaggaagat ttcctcccca tcgtcggttt
     3541 ggaaatgcac ccgcaaacgg gaacgggtgc cccggcgcat cattttgtgg ttctcaacgt
     3601 gccgcacccg ttcgagctgg atcgcaagct cgctgcgcag gcgatgatcg accaggtgct
     3661 ttccgtgggt ggcaaggtca tctatgccca cccctattgg acaactcact cgttgaacga
     3721 aatgctagag gtagccggtt actgtgcctt agaagtcttc aatggtggtt gccacgtgga
     3781 aaacgacaca ggctatggtt ccgtccattg ggaccaagtt ttgaacaaag ggcggctcat
     3841 cggtgggatt gccacggatg atgtccacaa ttccaaattg atcaacattg gctggacaat
     3901 gatcaaggcc cctgcactca atgcagcagc catcatggaa gcgatcgaaa aaggctgtta
     3961 ctatgcctcg gcgggaccga ccat
//
LOCUS       DAOPTR010000249         3982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_18160_length_3982_cov_3.740769, whole genome shotgun sequence.
ACCESSION   DAOPTR010000249 DAOPTR010000000
VERSION     DAOPTR010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18160_length_3982_cov_3.740769"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            368..790
                     /gene="rpsP"
                     /locus_tag="PKI05_05975"
     CDS             368..790
                     /gene="rpsP"
                     /locus_tag="PKI05_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOA51786.1"
                     /translation="MAVRIRMKRCGRKHRPFFRICATDARAPRDGRVIEVLGTYDPLI
                     PLTDARVTLNLERVKYWLSVGAQPSEKVKVLLKKYGPNGTHLEKQREAIEKLRQLQAA
                     RKAAAQHAAVLAEQKKAAKKALEDAARAAAEEAEAPAE"
     gene            794..1549
                     /gene="trmD"
                     /locus_tag="PKI05_05980"
     CDS             794..1549
                     /gene="trmD"
                     /locus_tag="PKI05_05980"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOA51787.1"
                     /translation="MRIDVLTLFPEIFSGYLGQSVLKKAIDRGLVQVLLHNIRDWSHD
                     KKHHKVDDRPFGGGPGMVLMAPPVVECVEAVQAQAEPGRLVMLTPQGRRLTQPIVEEL
                     ARHQRLLLLCGRYEGFDERIRLILKPDEISIGDYVLNGGEVAAMVMIDAVIRLIPGVL
                     GDEESSCLDSFSGPERLLDYPQYTRPRCYRGYEVPAVLLSGNHQAVRRWRLQEQRRLT
                     AQRRADLLQHGLSSEQCEHIVETPADPPSASGQ"
     gene            1655..2002
                     /gene="rplS"
                     /locus_tag="PKI05_05985"
                     /pseudo
     CDS             1655..2002
                     /gene="rplS"
                     /locus_tag="PKI05_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329361.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            2304..2684
                     /locus_tag="PKI05_05990"
     CDS             2304..2684
                     /locus_tag="PKI05_05990"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOA51788.1"
                     /translation="MASADDPRAQLGHAGEQAAMDFLIKRGHTILARNVRYPEGELDI
                     VTRDGQCLVFVEVRTRRSEKHGECWEGISRAKQRRLIRAAHRFRREGRLFSVPCRFDV
                     ITVLWPEGCHEPIVTHFQRAFDAD"
     gene            complement(2741..3568)
                     /gene="folP"
                     /locus_tag="PKI05_05995"
     CDS             complement(2741..3568)
                     /gene="folP"
                     /locus_tag="PKI05_05995"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOA51789.1"
                     /translation="MVDRWTIRNGTLDLTRRPLLMGILNVTPDSFSDGGRYFDHQAAI
                     EHGEQLAAEGADILDIGGESTRPGAEPVPLEEELRRVIPVVEALAPRLSIPISIDTSK
                     AMVARQAIIAGAAIVNDVTALRGDPDMVDVVRETGAGVCLMHMKGTPRTMQQEAVYED
                     VVSEVASFLLSRCRELESAGIPSDRLAIDPGLGFAKTPHQNWQIIQGLQQLRSLGYPL
                     VIGHSRKRFIREVVGNDFRSVLWGTVGVGLLLAAGGVDILRVHDVKAMREALALWRR"
     gene            complement(3575..>3982)
                     /locus_tag="PKI05_06000"
     CDS             complement(3575..>3982)
                     /locus_tag="PKI05_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51790.1"
                     /translation="FLVTVTVAGDSPHAVQILAWVYFLTFAVSVLAYTGYAFWMSGLS
                     LRSVLGTLHAIWLGLLLMIVAVWATRVGFTLLRFQSDYLLLFSEIVAGMIAYFIGTRR
                     ISLEYLIGSLKGFWPESQQGLPEREDRAESQEV"
BASE COUNT          903 a         1084 c         1157 g          838 t
ORIGIN      
        1 gtgccgactt aaacggatta accgatttga gagactttaa cggcacccca cctattagcg
       61 gtatatcatc gtatgttggg acactgccga ggcagtctct tgtttccggc acgcgggaat
      121 cgccactgtc tgcggcaggt agcgatattg gttggcactc cgcggcagtc tccccattgc
      181 aaaatagggt gaattttggc acattagaat tcttgcccca tgagtgaatc caccttcggg
      241 tggaggattg gcggcttggg ggttggaaac tcggtcgatc atcaaactga aggtatcacg
      301 ttaacgccgt aaaagaggat tttttgccga accgcgagta tttcggcgaa aaagggagaa
      361 agactacgtg gccgttcgca ttcgtatgaa aaggtgtggg cgaaagcacc gcccgttttt
      421 taggatttgc gccaccgatg cccgtgcgcc gcgggatggg cgtgtcattg aggtcctggg
      481 gacttatgat cctttgattc cactgaccga cgctcgggta acgctgaacc tggaacgggt
      541 caagtactgg ctcagcgtgg gtgcccagcc gtcggagaag gtaaaagtcc tgctcaagaa
      601 atacgggccc aatggcaccc atttagaaaa gcagcgcgag gccatagaaa agcttcgcca
      661 attgcaagcg gcgcgaaagg cagcggcgca acacgcggcc gtgctggccg aacagaaaaa
      721 ggccgctaaa aaggccttgg aagatgccgc acgggccgct gccgaagaag ccgaggcccc
      781 agccgagtga tccatgcgta ttgatgttct aacgcttttt cccgagatat tttccggcta
      841 tcttgggcaa agcgttctga aaaaggccat cgatcgcgga ctggtgcagg tccttttgca
      901 caatatccgc gactggagtc acgacaaaaa acatcacaag gttgacgacc ggccgtttgg
      961 tggtggacca gggatggtac tcatggcacc gcctgtcgtt gagtgcgtcg aggcagtcca
     1021 ggcacaagca gaacccggcc gcctggtcat gctgacaccc caaggccgtc gattaactca
     1081 gccgattgtg gaggaactcg cgcgacacca aaggctgcta ctgttgtgtg gacgctacga
     1141 ggggtttgac gagcggattc ggctgatttt aaagccggat gagatttcga tcggcgatta
     1201 cgtcctcaac gggggcgaag tcgccgctat ggtgatgatt gatgcggtaa tccggttgat
     1261 tccgggtgtc ctgggcgacg aggagagcag ttgcttagat tcgttttccg gcccggagcg
     1321 actgctcgat tatcctcaat acacgcggcc aaggtgttac cggggatatg aggtcccggc
     1381 cgtgttgttg agcggaaatc accaggctgt ccgccgctgg cggttacagg aacagaggcg
     1441 gctgacggcc caacgccgtg ccgatttact gcaacacggt ttgtcaagcg aacagtgcga
     1501 acatattgtg gaaacaccgg ctgacccacc atcggcaagt ggacagtgac gtttcggcac
     1561 cggagcgaat tcggcagcat cttaaaattg ctcagactgt ggtggggttg aaagagaaaa
     1621 cgaaaatcca cttataacca attcgaggct ggccatgaat aagggtgaat tgatcaaggc
     1681 tgttgagcaa acctgcttga agaccgacat cccctatttt gaagtcggtg atacggtaga
     1741 tgttcattgc cgtatcatcg aaggcgacaa cgaacggatc cagattttca acggcgtcgt
     1801 cattgcccga agtggttcgg gaacacggga aatgttcact gttcggcgga tcgtgcaggg
     1861 cgagggtgtg gagcgaaaat tccccctcca ctcgccgcgg atcgccaaga ttgaggtgaa
     1921 gcgagcggcg atcgtccgtc gagccaagct gtactttctc cgcgaacggg tggggaaggc
     1981 cgtccggttg cgcgagcgcc gtcctgacca gatgcagaaa cgtctcaatc gcgagaaggc
     2041 ctattgggcc aagcgggggg gacagcctca agaggcctaa gcccttcagc agccacaacc
     2101 tggcgatcgg catcacagtt tcgccggcaa ttgattgctt ttgggttgtc taaaacgtga
     2161 ccatcattaa gcggccagtt cgatcgtatg acgattcggg ccggccgtct ttcttttcgt
     2221 ctttgtggcg gttatttcgc cgtctggtag aattccttgt tcgccggcga tcgcgaactc
     2281 tgcttgcggc taaccagtgc gcggtggctt ctgcggatga cccgcgggca cagcttggtc
     2341 atgcaggcga acaggccgcc atggacttct tgatcaaacg tggccacacc atcctcgccc
     2401 gaaatgtgcg ttacccggag ggtgaactag acatcgtaac ccgtgacggt cagtgccttg
     2461 ttttcgtcga ggttcgtact cggcgatcgg aaaaacatgg ggaatgctgg gagggcatta
     2521 gtcgagccaa gcaacggcgt ctcatacgtg cggcgcatcg gtttcgtcgg gagggccggc
     2581 ttttctccgt tccctgccgc tttgatgtca tcacggtact ttggccagag ggctgtcatg
     2641 agccaattgt cacgcacttc caacgggcct tcgacgctga ttgattaaac cgagaagagt
     2701 tgtctcccaa tggtattacc gtgaggccta cggcttgatg tcacctccgc cataaggcaa
     2761 gggcctctcg catggccttc acatcgtgaa cacgcaagat gtccactccc cctgccgcca
     2821 ggagtaaccc tacaccgacc gtgccccaaa ggaccgaccg gaaatcgttg cccacaacct
     2881 cgcggataaa tcttttgcgg gaatgaccta tgaccagcgg ataacccaaa ctacgcagtt
     2941 gttgcaatcc ttgaatgatt tgccaattct ggtgcggcgt tttcgcaaat cctaagccgg
     3001 ggtcaatggc caagcgatct gagggaattc cggccgattc caattctcgg catcgcgaca
     3061 gcaggaaact ggcaacctcg ctaacgacgt cctcgtaaac agcttcctgc tgcatggttc
     3121 ggggcgtacc tttcatgtgc ataaggcata ccccggcacc ggtttcccgg acgacatcga
     3181 ccatgtcggg gtctcctcga agggccgtga catcgttgac gatagccgcc cccgcgataa
     3241 tggcctgtcg agctaccatc gccttggagg tatcaatgga gatgggaatc gaaagacgcg
     3301 gagcgagggc ctcaaccact ggaatgacac gcctgagttc ttcctcgaga ggcacaggct
     3361 cggccccggg ccgtgtgctt tcgccgccaa tatccaagat gtccgcccct tccgcggcaa
     3421 gttgttcgcc gtgttcgatg gccgcctgat ggtcaaagta ccgcccgccg tcggagaaac
     3481 tgtcaggcgt cacattcagg atccccataa ggagtggccg tcgtgtgagg tcaagcgtcc
     3541 cgttacggat tgtccagcga tcgaccatgg aacgttatac ctcctgcgac tccgcccggt
     3601 cttcgcgttc cggcaggccc tgctgggatt ctggccagaa ccctttcagg ctgccgatca
     3661 ggtactccag ggaaattcgc cgtgtcccga taaagtaggc aatcatgcct gctacaattt
     3721 cgctgaaaag gaggagataa tccgattgaa aacgcaacag cgtaaagccc acccgcgtcg
     3781 cccacacagc tacaatcatt agcagcaaac ctagccaaat ggcatgcagt gtgccaagca
     3841 ccgacctgag agaaagcccg ctcatccaga aagcgtatcc tgtataagcc agaacggata
     3901 cggcgaacgt caggaaatag acccaagcca agatttggac ggcgtggggg gagtcccccg
     3961 cgacagtcac cgtcaccaga aa
//
LOCUS       DAOPTR010000250         3974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_18205_length_3974_cov_3.196479, whole genome shotgun sequence.
ACCESSION   DAOPTR010000250 DAOPTR010000000
VERSION     DAOPTR010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18205_length_3974_cov_3.196479"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..641
                     /locus_tag="PKI05_06005"
     CDS             165..641
                     /locus_tag="PKI05_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51791.1"
                     /translation="MDREESQSTFLRHCALVEATAERLAAEGVGWTTFFRELLGPEGL
                     VRQLFPTPQQLSEFERTDTYQKVQEILAKLRRLQEGRSAPDEPLTVITIRVPRSLHEA
                     LAEEAHALKTSINRLCISKLAQLIDTRFVPPMRHLDREASHEEQELTQPPSRKTRK"
     gene            complement(658..1061)
                     /gene="rnpB"
                     /locus_tag="PKI05_06010"
     ncRNA           complement(658..1061)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKI05_06010"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1121..2152)
                     /locus_tag="PKI05_06015"
     CDS             complement(1121..2152)
                     /locus_tag="PKI05_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOA51792.1"
                     /translation="MGTNPDKRLGINSASSYNWRQLGYRYYSLSFYLNRIFGGKTWKV
                     SVDGGFHCPNADGTISHAGCLFCNILSFSPSRRRSGLTIREQLLQAATHLRERRGIQK
                     IIAYFQPGTNTYGPPDKLERLYREALSVPDVVGLAIGTRPDCLSEEVVKLLATLSQET
                     WLTVEIGVQSSHNATLRRINRGHSWEDAVNAIQRCANEGIRVTVHLMFGLPGETYQQM
                     LETAEKVAELPIHGVKIHNLYVAKDTGLAKLYEAGQYEPLSQDVYVNMVCDALERLPP
                     HIVIERLTAETDPGYLIAPDWCRRKGETISLIGKQLELRNTYQGCRFSEGPTQPDGLS
                     SSPNKCGST"
     gene            2262..2777
                     /locus_tag="PKI05_06020"
     CDS             2262..2777
                     /locus_tag="PKI05_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOA51793.1"
                     /translation="MYGELIPLGGGDPIPLLKKRLIVGRRESCDIVLRFSNVSAQHCE
                     LFVMSGYWYVRDLNSTNGIKVNGTRVREKRLDPGAVLSIAKHQYEIRYNPIELGAVGP
                     PPPDTLEQDIFTRSLLERAGLVKSKPVPEPEETEKTEPPSGDDGKRYDILEGFRKSEF
                     KKSWTEPQSPK"
     gene            2864..>3974
                     /gene="rsgA"
                     /locus_tag="PKI05_06025"
     CDS             2864..>3974
                     /gene="rsgA"
                     /locus_tag="PKI05_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011119119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOA51794.1"
                     /translation="MGKKKKIRVDFRKNRTSKPRQKDLTDRVVSRDEVLDEDLTREER
                     IGGRGELTRRRTVIGQETDDKSGVATPLPVILEPRHPDSRLGRVIAVQGLICQVEDAE
                     GQAYRCVIRRLLKRLATDQRNVVVVGDQVHFHPLPGSDGEGVIETILPRRGALARTSK
                     GKHHVLVANVDQAIIVSSAAMPRLKPHLIDRMIVAAEKSRIEPVICINKIDLVDPADL
                     EPLVGLYSQLGYRVLLVSAITGFGLPALIRTLKDKVSVLSGQSGVGKSSLLNAIDPSL
                     RIPVAPVSEDTEKGRHTTTTARLYRFHFGGYVADTPGIRQFQLWDLTPDEVVNYFRDL
                     RPYVNGCRFPNCLHLEEADCAVKDAVADGLIDERRY"
BASE COUNT          924 a         1073 c         1097 g          880 t
ORIGIN      
        1 ttgccacagt tgttcattgc tgttcgcata atgggttgtc aagtcttttc cggggacgcc
       61 tagacgaatc cgttatctcg tcaagaatac cccagaaaac ggtcgggaaa tccccggttc
      121 ggtcatcggc tgaatttcag accctgagtt tccggaggtg tgagatggat cgggaagaat
      181 cacaaagcac ctttctgagg cattgcgcgc tcgtagaggc tactgccgag cgacttgccg
      241 ccgaaggggt aggatggacc accttctttc gggagctgct tggaccggaa ggacttgttc
      301 gacagctttt ccccacaccc cagcaactta gcgaattcga acggacagat acctaccaaa
      361 aggtccagga aatccttgcc aagctccgcc gactccagga aggccgttcc gcaccggacg
      421 aaccattgac tgtgattacc attagggtcc cgcggagcct gcacgaggca ctggcagagg
      481 aagctcacgc tttgaagaca agtatcaacc gcctttgtat ttcaaaactg gcccaattga
      541 tcgacacgcg atttgtgccg ccaatgcgcc acttggaccg agaggcatcc cacgaagaac
      601 aagagcttac gcagccgcct tctcgtaaaa cacggaaatg aaaaaatgaa agacgaggac
      661 aagggcggac ggtaagccgg gttctgttct tcgagaaact cgaagtgatg gccatttatc
      721 taggaggccg attgctcgac ccctcaagcg gcctacccgg aagtgatata gcggtcggga
      781 tcacccgcag ggcctcactt gcgcgaggcc cactccttcc tgctcggcct tgctcccggt
      841 ggggtttgcc tagccaggcc ggtcacccga cctgctggtg agctcttacc tcaccctttc
      901 acccttaccg cgcggaaacc cgcgaggcgg tctcgtttct gtggcacttt ccctagcctt
      961 tcggccggtg ggtgttaccc accaccgtga tcccgtggag cccggacttt cctctcgccg
     1021 tcacttctga aatgacggcc agcggccatc tcgtccgctc tgtcctcgtc cacatgaatc
     1081 atatgaccaa gacccgatgg aagaaaggag tgtgcctcgc ttacgtactt ccgcacttat
     1141 ttgggctcga agaaaggccg tccggctgag tcggcccctc gctgaaccgg catccttgat
     1201 atgtattacg gagttctagt tgtttcccga tgagcgagat cgtttcaccc ttccgacgac
     1261 accagtctgg tgctatgaga taacccggat ctgtctccgc cgtcagtcgt tcaatcacga
     1321 tgtgcggtgg aagcctctcg agggcatcgc ataccatgtt cacatagacg tcttgggaaa
     1381 gcggctcata ttggccagcc tcgtaaagct tagccaatcc tgtatctttt gcgacgtaaa
     1441 gattgtgtat ttttacgccg tggatcggca gttccgcaac cttttcggcc gtctccagca
     1501 tctgttggta agtctcaccc ggaaggccaa acatgaggtg aacggtaaca cgaattccct
     1561 cattcgcaca acgctgaatg gcgttcacag cgtcttccca ggagtggccc cgattaatcc
     1621 gccgaagtgt agcattatgc gaagattgaa ccccgatttc cacggttaac caggtttcct
     1681 gactgagtgt tgcaagcagc ttgaccactt cttccgagag gcagtcggga cgggtcccaa
     1741 tcgccaaacc aaccacgtca ggcacggaga gcgcctcccg gtatagccgc tcgagcttat
     1801 caggaggacc gtaggtgttc gttcccggtt gaaagtacgc gatgatcttc tgaattccac
     1861 gtcgctctcg caggtgagtg gcggcttgca gcagttgttc cctgatggtt aggccagacc
     1921 gccgtcggct cgggctaaag ctgaggatat tgcagaagag gcaaccagcg tgactgatgg
     1981 ttccgtccgc attggggcag tggaaaccgc cgtccacgct gactttccaa gtttttcccc
     2041 cgaaaatacg attgagatag aagctgagac tataataacg gtagccgagc tgccgccaat
     2101 tataagagga ggcggaattg attccgagtc ttttgtccgg gttcgtgccc atttgttgac
     2161 ggcaaagtta accaagctta cactgaaaga cgacgttgta aacactgtca tcctaagagg
     2221 aagaatattt tgcgacaagg aactgagaac aggcagaact catgtacggt gagctgatcc
     2281 cgctgggtgg gggagacccc atacctctct tgaagaaacg tctcattgtg gggcggcgag
     2341 aaagttgcga tatcgtcctg cggttctcca acgtgtccgc gcaacattgc gaacttttcg
     2401 taatgtccgg atactggtac gtgcgggacc tcaatagcac gaatggtatc aaggtcaacg
     2461 ggactcgggt tcgggagaaa cgcctggatc ccggtgccgt attgtcaata gcgaagcacc
     2521 aatacgaaat tcgatataat ccgattgagc tgggcgccgt cggccctcca ccgccggaca
     2581 cgctagaaca ggacatattc acacgttccc tcctggaaag ggctggcctc gtcaaatcga
     2641 agccggtccc ggagccagag gaaacagaaa agaccgaacc gccctcaggg gacgatggga
     2701 aacggtacga cattttggag ggcttccgga agtccgaatt caaaaagtcc tggacggagc
     2761 ctcaatcacc gaaatgacaa ggccaagaaa cccacgaaac ccgccgaata acagggagag
     2821 tttccgcatg ctcctgcccg caagcttttg ccgggtgttg tgaatgggaa aaaagaaaaa
     2881 aatacgtgtc gatttcagaa aaaatcggac ttcaaaacct cgccagaagg atctcactga
     2941 tcgtgttgtc agccgtgatg aggtccttga cgaagatttg acacgcgagg agcggatcgg
     3001 cggacgaggg gagttgaccc gacgaaggac cgtcatcggc caggagaccg atgataaatc
     3061 cggagtcgcg actcccctgc cggtgattct tgagccacgt cacccggata gtcgccttgg
     3121 tcgcgtgatt gccgttcagg gtcttatatg ccaggttgag gacgccgaag ggcaggctta
     3181 tcgttgtgta atccgtcgcc tcttaaaacg cctggcaacg gatcaaagga acgtggtcgt
     3241 cgttggtgac caagttcatt ttcacccgtt accgggctca gatggcgaag gagtgatcga
     3301 aaccattctg ccgcggcgcg gcgcccttgc gcggacttcc aagggcaagc accatgtcct
     3361 tgtggcaaat gtcgatcagg caatcatcgt tagcagcgct gcgatgccgc ggttgaagcc
     3421 acatcttatc gaccgcatga ttgtggccgc cgaaaaatct aggatcgagc cggttatctg
     3481 catcaataag attgatctgg ttgatccagc cgacttggaa ccgctggtcg gactttacag
     3541 tcaattggga taccgcgtgc tgctggtcag tgcgatcacg gggtttggtc ttcctgcact
     3601 gatccgaaca ctcaaggaca aagttagtgt cctctccgga caaagcgggg tgggaaaatc
     3661 ctcattgctc aacgcgattg atccatcgct tcgaattcct gtcgcgccgg tgagtgaaga
     3721 cacggaaaaa gggcgacaca caacgacgac tgctcggttg tatcggttcc atttcggagg
     3781 atacgtggca gacactcctg gtatccggca atttcagttg tgggatttga cacctgacga
     3841 ggtggtcaat tattttcggg acctaagacc ttatgtgaat ggatgccggt ttcccaactg
     3901 ccttcatttg gaagaggcag attgcgccgt gaaggacgcc gtggctgacg gtttgattga
     3961 tgaacgccgc tacg
//
LOCUS       DAOPTR010000251         3974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_18206_length_3974_cov_2.928553, whole genome shotgun sequence.
ACCESSION   DAOPTR010000251 DAOPTR010000000
VERSION     DAOPTR010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18206_length_3974_cov_2.928553"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(421..2019)
                     /locus_tag="PKI05_06030"
     CDS             complement(421..2019)
                     /locus_tag="PKI05_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOA51795.1"
                     /translation="MIRTNQAVHGTSVMRHSVTTWISRRVERVLRATALVAIAVVCSA
                     FSFCWAEQNPQGKERPNIVIILADDLGYGSVECYGAPADLVRTPNIDRLAREGRRFTD
                     ANTPSSVCTPTRYALLTGRYCWRTDLKHEVASVFSPLLIEPGRINLASMLKKYGYETA
                     AIGKWHLGYGTGPRTDYTKELRPGPLEIGFDYHFAVPSNHGDITGVYVENHHVYGLRS
                     DKLNPTNLGTNFNKRPYLGLDAPQRVDEEVMPVLTTKAEQWIESRSGNKPFFLYFTPV
                     AVHNPVTPSEKTKGTSKAGPYGDWIHELDLSVGRILDVLDRKGFIPNTLVIFTSDNGG
                     VNKPTIPSESTDALKAGLPICGPFRGGKHDVWEGGFRVPFIVRWPGHVTPGTVCDEMM
                     SLTDVLATVAAIMGENLPPPDVGAEDSYNMLPAWLGQPYSSPIRPDMIVHSADGNFAI
                     RRGKWKWIEGTYHPDTRPGALRLRADQFHPQLYDLSTDIGETHDVATEHPEVVAELSR
                     LLNHYRDCGHSRQVTPPDQVRREN"
     gene            2398..3840
                     /locus_tag="PKI05_06035"
     CDS             2398..3840
                     /locus_tag="PKI05_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome B6"
                     /protein_id="HOA51796.1"
                     /translation="MKAVSIRKWACLFAPVVAAALVGVWTSGHLRSAEPTRNVETVAP
                     VGATSYARVDTDEPFPAVVQRMQAQKEQILSRHRQLLEERYDLSNRPLPGAVMSRGKP
                     IQAGPRVKLPPGLTWEQLAEMSPEVIKQKGLWPKGFLPLPHPNHDEGGMLFPQHHIDE
                     IKRQEGRDLTRFDLDYDLPEHFLPEYPPAIYLTTRPDLGDVSRGQVITLQNYFQLFNG
                     ILNPKQLEGLRLLVTPFPQQQFNATDDRRSLRPSQGVTCFDCHVNGHTNGTTHLLGDI
                     RPQAFRPRLDTPSLRGVNVQRLFGSKRALKTIEDFTEFEQRAAYFDGDPVIATKKGVN
                     VLERGSQVHFMAEFMAILDFPPAPKLDVFGRLDPTKATHQELRGEQVFFGKGRCAECH
                     PAPYYTDNLMHDFHTERFFHPHVVNGHVMGGDGPIKTFPLRGIKDSPPYHHDGRLLTL
                     EDAIEFHNLVLGTRLTAREKEDLAAFLRCL"
BASE COUNT          889 a         1111 c         1098 g          876 t
ORIGIN      
        1 aatgaccaag ccgagatccc tagcgaaaac acaactgccc aagcgactcc caaaacaagc
       61 tcaaaccaat gccttcgaca cagcgacttc atatctcgca ctcccatctt gccccagtga
      121 gggccacgac tgtttatgac agcttcgtta acggactggc taccacagct tcgtaaccga
      181 cgctacgcga gtcggaaatg cccttttctt cgggctattg ccagtctaat caaacacccc
      241 ggacgcggtc aacgtgcaat gttaggtcta cttgtcccga cccgtagccg aacgaactac
      301 agagggttct acggtgttca ccccgaatct atgaaattag cggggtcagt gcccgaatgt
      361 gggaatctat atcgcagatc gccgggtcgc aataagctta cagacctcgc caagattacc
      421 ctaattctcg cggcggactt gatccggtgg ggttacctgc cgactatggc cacaatcgcg
      481 atagtggttc agcagacggg atagctcggc cacgacctca gggtgttccg ttgccacgtc
      541 gtgtgtttcc ccgatgtccg tggagagatc ataaagctgt gggtgaaatt ggtcggcccg
      601 taaccgcaag gcgccaggac gggtgtccgg atgatatgtg ccctcaatcc atttccactt
      661 tccccggcgg atggcgaagt taccatcggc actgtgaacg atcatgtcag gacggatggg
      721 cgaggaatac ggctgcccga gccaggcggg tagcatgttg tagctgtcct cggcaccgac
      781 gtcgggcgga ggtaggtttt cacccataat ggctgccacc gtggccaaaa catcggtgag
      841 actcatcatc tcgtcgcaca ccgtaccggg cgttacatga ccaggccaac ggacgataaa
      901 aggtacccgg aaaccacctt cccacacatc gtgctttccg ccgcgaaacg gcccacagat
      961 cggcagtccc gccttgagcg catccgtgga ttcactcggg atggtgggtt tgttcactcc
     1021 gccgttgtcg ctggtgaaga taacgagcgt gtttggtatg aagcctttac gatccaacac
     1081 atccaggatt cgccccacac taagatcgag ttcgtggatc caatcaccat agggaccagc
     1141 cttacttgtg cctttcgtct tttccgaagg tgtcacaggg ttatggaccg ccaccggcgt
     1201 gaagtagaga aagaacggct tgttgcccga gcggctctcg atccattgct cggcctttgt
     1261 cgtaagaacc ggcatcacct cttcatcgac tcgctgtggg gcgtcgagac cgaggtacgg
     1321 tcgcttgttg aaatttgtgc ccagattcgt cggattgagt ttatcggatc ggagcccata
     1381 cacgtggtgg ttttccacgt aaacacctgt gatatcccca tgattcgaag gcacggcgaa
     1441 gtgatagtcg aagccgattt cgagtgggcc aggacgcaac tctttggtgt agtcggtgcg
     1501 ggggcctgtc ccataaccca agtgccactt cccaatggcg gccgtctcgt atccgtattt
     1561 cttcagcatc gatgccagat tgattcgccc tggttcaatg agaagtggtg aaaatacact
     1621 ggcgacctcg tgcttgaggt ccgtgcgcca gcaataccga ccggtaagca acgcatagcg
     1681 agttggcgta caaacggagg aaggagtgtt cgcatcggta aatcgccgac cttctcgtgc
     1741 cagacgatcg atgttcggcg ttcgcacaag atcagcaggg gctccgtaac attccacact
     1801 gccgtaaccc aaatcgtcgg ctaggataat cacgatgttg gggcgttcct tgccttgtgg
     1861 gttttgctct gcccaacaga aagaaaaggc actgcagacg actgcaatcg ccacaagcgc
     1921 tgtggctctc aaaaccctct ccactcttct tgaaatccac gttgtcacgc tatgtcgcat
     1981 gacagacgtg ccatgcaccg cctgattggt gcggatcatg acaaacctcc ccatatctga
     2041 gctaaactgc gatgaactag caattctttc gcactggcgc atttgtagtc acgtccacta
     2101 cgtctgtcaa tcgccactat cgccggtaaa atcgcttgca ctcgtggtgg gcggcatcaa
     2161 tttgggaggt tgtatcgcga acacagccat gctgcccgga gacgccagtg ccaaagccaa
     2221 tcttctatct tcgtcgggca attcgtggac aatccacttg ttttttgagg tgtggccgcc
     2281 tagaatttgg gcagtcggga aagcgggaac gcgcgtatgg gtgatcctaa cgacgtgcgg
     2341 tccggacgcc ttgggggaaa agacgaactc ttgttaggga agaggaggga cctcacggtg
     2401 aaagctgtgt ctattagaaa atgggcgtgt cttttcgcgc ccgtcgttgc ggctgcgttg
     2461 gtaggcgtgt ggaccagtgg gcatcttcgc tcggcggaac caacaaggaa tgtggagacc
     2521 gtggctccgg tcggtgcaac cagttatgcg cgggtcgata ccgatgagcc ctttcccgcg
     2581 gtggtgcagc ggatgcaagc ccaaaaagag caaattctat cgcgtcaccg acaactgcta
     2641 gaggaacgtt atgacttgag caatcgcccg ttgccgggag ccgttatgtc gcgtggtaaa
     2701 ccgattcagg cggggccacg ggtgaaactc cctcctgggc tgacctggga gcagttggcc
     2761 gaaatgagcc ccgaggtaat caaacaaaaa ggcctttggc caaagggctt tctgcccttg
     2821 ccacatccga atcatgatga aggtggaatg ctcttcccac agcatcacat cgacgagatt
     2881 aagcggcagg aggggcgaga cctgactcgc tttgatctgg attacgattt gccggaacac
     2941 tttctgcccg aatatccgcc agcgatttat ttgacgactc ggccggacct gggcgatgtt
     3001 tctcgcggcc aggtgatcac gttgcagaac tatttccaac ttttcaatgg cattttgaac
     3061 ccaaagcagt tggaagggct tcggctcctg gtcacgccgt ttccgcagca acagtttaac
     3121 gccacggacg accggcgaag cctgagaccg agccaagggg taacctgttt cgattgccat
     3181 gtcaatggtc acaccaatgg aacgacgcac ctattgggcg acatccgacc tcaagccttt
     3241 cgacctcggc tcgatacgcc ctcgctacga ggggtgaacg tccaacggtt gtttggctct
     3301 aaacgggctt tgaagacaat cgaagacttt accgagtttg agcagcgagc cgcctatttt
     3361 gacggtgatc cggtcatcgc gacgaaaaaa ggcgtcaacg ttctcgagcg gggaagccag
     3421 gtccatttca tggcagaatt tatggcgatt ttggactttc cgccggcacc gaagttagac
     3481 gtctttggtc gattggaccc caccaaggcc acccaccaag agcttcgcgg agagcaggtc
     3541 ttttttggta aagggcgatg tgccgaatgc cacccggccc cctactacac agacaacctg
     3601 atgcacgatt tccataccga gcgattcttc catccacacg tggtgaacgg ccatgtgatg
     3661 ggcggggatg gcccaattaa gacattccca ctccgcggga tcaaggattc gccgccttat
     3721 caccacgatg ggcgactcct cacattggaa gatgccatcg aatttcacaa ccttgtcctg
     3781 ggcacccgct tgactgccag agagaaagag gacttggccg ctttcctgcg gtgcttgtga
     3841 gaacgagcaa ttggccgagg cggtttgact gaccagccct agggcgaaca cattaagtcg
     3901 cccttgatgt acggcggggt tgtgcccgac acgtgcccca tcaaaaccgt aggggcaggc
     3961 ccctgtgcct gccc
//
LOCUS       DAOPTR010000252         3972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_18217_length_3972_cov_2.649477, whole genome shotgun sequence.
ACCESSION   DAOPTR010000252 DAOPTR010000000
VERSION     DAOPTR010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18217_length_3972_cov_2.649477"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /locus_tag="PKI05_06040"
     CDS             <1..1316
                     /locus_tag="PKI05_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOA51797.1"
                     /translation="LETPNLDRMAREGAHCVNAFVATSLCSPSRASILSGLYMYHHQV
                     VDNQRPVPPGTVFFPQILQQAGFQTAFIGKWHMGHDDDSPQPGFDYWASFRGQGEYFD
                     PILNINGNRRQIKGYNTDVLTDLAIDWLKRGRDPSKPFFLYVGFKAVHYPFTPAPRHQ
                     GRYHGKRIDYPETMANTEQNYRSQPKWVRERRFSIHGIDHMETGQFDKDPVPNFDELY
                     WNYCETVFSLDEDIGRILDAVAAQGLDQNTVIIYMSDNGFALGEHGFYDKRDAFEVSI
                     RVPLLVYAPGLIGPGTKIEAMVQNIDIAPTVLELCGVALPATKPFDGRSFLPLLLGQQ
                     VPWRDHILYEYYWEWNFPATPTMFAIRTDRYKYVYYHGVWDYDSFHDLQTDPHERYNL
                     IDVPAYQELIQKLKQQLFDELESSGALMMPIRPPAGERLDQRKLE"
     gene            complement(1389..2570)
                     /locus_tag="PKI05_06045"
     CDS             complement(1389..2570)
                     /locus_tag="PKI05_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA51798.1"
                     /translation="MTAGTSWLLLSRGLHAQEPVTKKIRIGVVGGRFGATFQWHEHPQ
                     CVVAAVSDLRPERRSALQKAYQCDRVYDSLEEMLKEATDLDAIAIFTEAPNHVKHTVA
                     AMNHGKHVVCAVPAAMTLEECQTLLDTVKKTGLTFMMAETSYWQQHTISARKFVEQGE
                     FGEIFAMYSMYRHAGLESLWFEDGKPTWRHGFPPMHYPTHCTAHCIGVTGERLVAVSC
                     TGWGDDSPLLKGNPYNNPFWNETAEFVTDKGHSFRVEVWWAGAHVGCERAEWYGTKMS
                     FICENRLGTQATLIFAGEQKELDSGGFVRAASRVEPYEIPQWWATEMLPEPLRHDSGH
                     EGSHTFITHEFIDALICERRPAVDVYEAIAYTAPGIVAHQSALRDGERLTIPDFGKAP
                     A"
     gene            2883..>3972
                     /locus_tag="PKI05_06050"
     CDS             2883..>3972
                     /locus_tag="PKI05_06050"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOA51799.1"
                     /translation="MAKAQGLPPRSARKPEDCWDLSSLFPSDEAWEAALHAWEEAIPG
                     YERFRGKLSQGANVIAECLEFDYRMDREGERLGTYAFLKTAEDTTNPHYQRMRGRFLS
                     IATRANQAASFIRPELLAIPRRKMEQFLKDAALRSYEVPLRRILRWRPHTLGASEERI
                     LAMQTQMAETPSQIFHQLNDGDLRFGYVKDEKGRARELTHGNFLSFLQSPDRAVREAA
                     FETFYEKYAEHQNTLAATLSGSVQKDVYYARVRNFPSAREAALFPDNIPTQVYDQLIE
                     TVRRYLPSLHRYYDLRRRKLRLRQLCVYDTYVPLVPRLRLRTSWDQAVALVLKALAPL
                     GDEYVSILEKGFQSRWCDRYENRGKQSGA"
BASE COUNT          915 a         1071 c         1072 g          914 t
ORIGIN      
        1 tcttggagac gcctaaccta gatcggatgg cccgtgaagg tgcacattgt gttaacgcat
       61 ttgttgcaac atcattatgc tctccgagcc gggcttcgat tttgtcgggc ctgtatatgt
      121 accatcatca ggttgtcgac aaccaacggc ctgtgcctcc ggggaccgtg ttctttcctc
      181 agatccttca gcaagccgga tttcagacgg ccttcattgg aaagtggcac atggggcatg
      241 atgacgattc gccccaacct gggtttgact actgggccag ttttcgcggg caaggggaat
      301 attttgatcc gattctgaac atcaatggaa atcgaagaca aatcaagggg tataacaccg
      361 acgtgctgac cgacttggcc atcgactggt tgaagcgagg acgtgaccct tccaagccgt
      421 tcttccttta cgtgggcttc aaggcagtcc attacccctt cacaccggcg cctcggcacc
      481 aggggagata tcacgggaag aggatcgact atcccgaaac gatggccaac actgagcaga
      541 attaccgctc gcagcccaaa tgggtccgag aacgacgatt tagcattcac ggtatcgatc
      601 acatggagac cggtcaattt gacaaggacc ctgtgccgaa ttttgacgaa ctctattgga
      661 attactgtga gacggtgttt tctctggacg aagacatcgg ccgaattctt gatgccgtcg
      721 ccgctcaagg cttggatcaa aataccgtta tcatttacat gtccgataat ggctttgccc
      781 taggagaaca cggcttctac gacaagcggg atgcctttga ggtctcgatc cgcgttccgc
      841 ttttggtcta tgcacctggt cttatcggtc ccgggacgaa gattgaggcg atggttcaaa
      901 atatcgacat tgctcctaca gtcctagaac tttgtggggt ggctctgccc gccacaaagc
      961 ccttcgacgg tcggtcgttc ttgccgctgc tgttaggtca gcaggtaccg tggcgggacc
     1021 acattctcta cgagtattat tgggagtgga attttccggc tacgcccacg atgttcgcaa
     1081 tccgcaccga ccgttacaag tacgtctatt accacggcgt ttgggattat gacagttttc
     1141 atgatcttca gacggacccc cacgagcgat acaacctcat cgatgtgcca gcctatcaag
     1201 aattgatcca gaaactcaaa cagcaactct ttgacgagtt ggaatcctcc ggggcgctca
     1261 tgatgccgat aaggcccccg gctggggaac gtctcgacca acggaagctc gagtgaatct
     1321 tgtaccgcgg gtgtagctac gaactgttcg gcaaaaaccc gtgtcggcca actgctgagg
     1381 cgggtccgtc aagccggggc ctttccaaag tctggaatcg tgagccgttc gccgtcgcgc
     1441 aaggccgatt gatgtgccac aatccccggg gctgtgtagg cgatagcttc gtacacatcc
     1501 actgcgggtc gccgttcgca aatcagagca tcaatgaatt catgtgtgat gaacgtgtga
     1561 gatccctcgt gccctgaatc gtgccgcaaa ggttcgggaa gcatctcggt tgcccaccat
     1621 tggggaatct cgtaaggctc aacgcgcgat gcagcacgga cgaatccgcc gctatcgagt
     1681 tccttctgct cgccggcaaa gatgagggtc gcctgggttc ccaggcggtt ttcgcagatg
     1741 aagctcatct ttgtgccata ccattcggcc cgttcgcacc ccacatgggc ccctgcccac
     1801 cagacctcga cacgaaaaga gtgtcccttg tcggtcacga actcggccgt ttcattccag
     1861 aaggggttat tgtagggatt acccttaagg agtggtgaat catcacccca gcccgtgcag
     1921 gagacggcca caaggcgttc gcccgtcacg ccaatacaat gcgccgtgca atgggtagga
     1981 tagtgcatgg gaggaaaacc atgtcgccat gtcggcttgc catcttcaaa ccaaagcgat
     2041 tcaagtcccg catggcgata cattgaatac atggcaaaga tttcaccaaa ctccccctgc
     2101 tcgacgaatt tacgtgccga aatcgtatgc tgctgccagt agcttgtttc ggccatcatg
     2161 aaggtcaggc cggttttctt gacggtatcg agcagggtct gacactcttc cagggtcatc
     2221 gcggctggca cggcacaaac cacgtgtttg ccgtggttca tggcagcgac agtatgcttc
     2281 acatgattgg gggcctcggt aaaaatggca attgcgtcga ggtccgtggc ttctttgagc
     2341 atctcttcca gcgaatcata gacgcgatcg cattggtagg ccttctgaag ggccgaccgc
     2401 cgctctggcc gcaaatccga aacagcagcc acaacgcatt gcgggtgttc gtgccactgg
     2461 aatgtcgccc caaatcgccc accgaccacg ccgatgcgaa tcttcttcgt taccggttcc
     2521 tgagcatgca agccgcggct aagtaacaac caggaggtcc cggccgtcat tttaaggaat
     2581 gaccgcctcg attgctcatc tttgtgcacc aacccaattg acgtacgtct tgagttcttt
     2641 ctggtcataa aaagaccctc tacgaacaat gtgttgctat ggcttaatgt cccgtcgcgg
     2701 ttcggcttct tcgtaatact ggaccgaaaa gcggctttct cggtccgaag tgaccccgcg
     2761 ttggcgctac ggcacaaaca aaccataatc cgcaaaagga cgccgtacga gccccaatta
     2821 tgggagatcg gcagcacgag atcaagaaca aggtgcgata caaactcagg agaggatgtt
     2881 ccatggcgaa agctcaaggt ttacctccgc gaagtgctcg aaaaccggaa gattgctggg
     2941 atttgtcaag tctttttccg agtgatgagg cctgggaagc agcgctccat gcatgggagg
     3001 aagcaattcc cgggtatgag agatttcgtg gaaagctgag ccaaggtgcg aatgtcatcg
     3061 ccgaatgctt ggaattcgat tatcggatgg atcgtgaagg cgaacggctg gggacttacg
     3121 ccttcctcaa gaccgccgag gacacgacta atccgcacta tcagaggatg cgcggtcggt
     3181 tcttgagcat cgcaacccgg gcgaaccagg ctgccagttt tattcggcca gagctgttgg
     3241 ccatccctcg gaggaaaatg gagcaatttc tcaaggatgc cgcgcttcgt tcatacgaag
     3301 tgccgttgcg acgaattctc cgctggagac ctcacacgct gggggcatca gaagaacgaa
     3361 ttctggcgat gcaaacacaa atggctgaga ccccctctca gattttccat caactcaatg
     3421 atggtgattt gcgatttggc tacgttaagg atgagaaggg ccgggctcgc gaactcacgc
     3481 acggaaattt cctaagtttc cttcagtcgc cagatcgagc tgttcgagaa gcagcgtttg
     3541 agacatttta cgaaaaatac gccgagcacc aaaacacgct tgcggccacg ctatcggggt
     3601 ccgtgcagaa agacgtctat tacgcaaggg ttcgtaattt tccctcggcc cgtgaggccg
     3661 cattgtttcc tgataatatc cccacacagg tgtatgacca gttaatagag accgtccgtc
     3721 ggtacctgcc atcgttgcat cgttactacg acctccgccg gcggaagcta cgtctgcgtc
     3781 agttatgtgt ttatgacaca tatgttcccc tggtccctag gctaagatta cggacaagct
     3841 gggaccaagc ggttgccctt gtactaaagg cccttgcgcc ccttggtgat gagtatgtga
     3901 gtattctaga aaaggggttc cagtcacggt ggtgcgatcg ctacgagaac cgtgggaagc
     3961 agagtggggc at
//
LOCUS       DAOPTR010000253         3965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_18251_length_3965_cov_3.789003, whole genome shotgun sequence.
ACCESSION   DAOPTR010000253 DAOPTR010000000
VERSION     DAOPTR010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18251_length_3965_cov_3.789003"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PKI05_06055"
     CDS             <1..524
                     /locus_tag="PKI05_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51800.1"
                     /translation="WIVCSCGRQQVCLLKSQSGNPEGPYDMEGPPSLHGSTAAILDSG
                     SPTESRTDGYMFAYDPSLFVEDDGTIYLVFGPGWIAKLHEDLGRGLAETPRLLEIEGP
                     RLYAGTGGCALRKEAGKYILTALTEWNDTVEYVADSLYGPYRNPRLVLAQSRHVGFFK
                     SIDGEWTAVVSSY"
     gene            544..1713
                     /locus_tag="PKI05_06060"
     CDS             544..1713
                     /locus_tag="PKI05_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51801.1"
                     /translation="MNISAKLIAALTVLAGLYAVADAQSPGKKDTLRVFYFGNSLTAS
                     SMPELHEELGRSAGKVWKCDAFLGAGWQSWQHRNELWRAMGRPLDAATQSASSRGDLT
                     IDESLVKSAPMKPKAFLSGEWDAIVIQIFGSRLHYVTDEMWGMKFNGPIDVGDVAAAS
                     DIIRIFLKKNPQGRVYIYTVWPNMPSGKVPPDDQLPDWAKEMKKRFGSIRSAEFPDRA
                     GFDYAAQWEKPYQGDFDRPWIGHISRTRDYTNQVFEGIKRNFPELWDQRRLACIPAGE
                     LFFELDKRMQAGHMPGITTIKDFYTDVQHIRAGLPAYSIAALFYTVLFEEEPDSLDFT
                     IYNDPQKYGPDPHHDRGELLPITPERAKVVNETIWALVQRHPFTHLAQHSEAEKR"
     gene            1974..2579
                     /locus_tag="PKI05_06065"
     CDS             1974..2579
                     /locus_tag="PKI05_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51802.1"
                     /translation="MTSLTRIHTWAAVFMVGVVCGVLLSTHWPQTPIRAVATDRGQNF
                     AVATGPVDAGVEAFFFLDFLTGQLRGVVLSNQTRNFQTTYEANVFADLTTVIQLKNAE
                     IQKLNAQAARSGGPTRPEIQIPQSPNYLLVTGLADIRRGPSVGVRPGQTMVYVTETNT
                     GIVLAYMVPWSPERHSANQPFSTPLQLWAAEQFSTVVLRTE"
     gene            2704..2931
                     /gene="thiS"
                     /locus_tag="PKI05_06070"
     CDS             2704..2931
                     /gene="thiS"
                     /locus_tag="PKI05_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOA51803.1"
                     /translation="MANHPDSQTIRILINGEWREVASGITLADLLKELKLADKPVAVE
                     RNLQVVPKARHPETPLADGDRLEIVTLVGGG"
     gene            2939..3781
                     /locus_tag="PKI05_06075"
     CDS             2939..3781
                     /locus_tag="PKI05_06075"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011120018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HOA51804.1"
                     /translation="MATHDFDSGLRIGSHVFKSRLIVGTGKYSSFEVMQQALELSGTE
                     CITVAVRRERLFDQQGRNILDFIDTKRYTILPNTAGCFTAEDAVRVARLGREILTQLG
                     NPGADWVKLEVLGDKGTLLPDPIATVQATEILVKDGFQVLVYTTDDPIVAKRLKEAGA
                     TSVMPAGSPIGSGQGILNPNNIKICLEYLKENDPDYPVIVDAGVGTASDVTVAFELGV
                     DGVLLNTGIAHAKDPLRMAVAMKYAAEAGRLAYLAGRIPKKLYATASSPLEGTIAPSH
                     EAGK"
BASE COUNT          868 a         1055 c         1175 g          867 t
ORIGIN      
        1 attggattgt ctgttcctgc ggccggcaac aggtgtgttt gctgaaaagt caaagcggaa
       61 acccggaggg gccctacgac atggagggtc ccccatccct gcacggctcg actgccgcca
      121 ttttggattc cggcagccca acggagtcgc gcacggacgg ctacatgttc gcctacgacc
      181 ccagcctgtt cgtggaggat gatggcacta tttacctcgt cttcggtccc ggttggattg
      241 ccaagctgca cgaagacctt ggccggggtt tggcagaaac gccgcggtta ctggagatcg
      301 aaggccctcg cctctacgct ggaacaggtg gctgcgcgct gcgaaaagaa gctggtaagt
      361 acatacttac cgcattgacc gagtggaatg atacggtgga atacgtcgcc gactcgctgt
      421 atggacctta ccgcaatcct cgcctggtac tggcgcagtc cagacatgta ggtttcttta
      481 agtccataga cggcgagtgg accgcagtcg ttagcagtta ctgaccgaca aggagtaaag
      541 gatatgaaca tttccgcgaa gctaattgct gctctaaccg ttctcgccgg cctatatgca
      601 gttgccgatg cgcagtcgcc aggcaagaaa gacactttgc gtgtgtttta tttcggcaac
      661 agcttaaccg ccagctccat gcctgagttg catgaggaac tgggacgcag cgcgggcaag
      721 gtctggaagt gtgatgcctt cctgggtgcg ggctggcaat cgtggcaaca ccgtaacgaa
      781 ctgtggcgag ccatgggacg gccactcgat gctgccacgc aaagtgcttc cagccggggc
      841 gacctgacta tcgacgagag tctcgtcaag tctgcaccga tgaaaccaaa ggcgtttctc
      901 agcggtgagt gggatgcgat tgtcatccaa atctttggtt ccaggctcca ttatgtcacg
      961 gacgaaatgt gggggatgaa gttcaacgga cccatagacg tgggcgatgt ggcggccgcc
     1021 agcgacatca tccgtatctt tctcaagaaa aatccgcaag gcagggtata tatctacacc
     1081 gtctggccga acatgccgag cggcaaagtc ccacccgacg accaattgcc ggactgggcg
     1141 aaagaaatga aaaaacgatt cggcagcatc cgttccgccg aatttccgga ccgtgccgga
     1201 ttcgattacg ccgcgcagtg ggaaaaacca tatcaaggcg atttcgacag gccctggatc
     1261 ggacatatct ctcggacccg cgattacacc aaccaggtgt tcgagggcat caaacggaac
     1321 tttccagaac tctgggacca gcgtcggctg gcgtgcattc cggccggcga gttgttcttc
     1381 gaactggata agcgaatgca ggctggccat atgccaggca tcacgacgat caaggatttc
     1441 tataccgacg tccagcacat ccgtgccggt ctgcccgcat atagtatcgc cgctctgttt
     1501 tacacggtgc tgtttgaaga agagcccgac tcgcttgatt tcaccatcta caatgacccg
     1561 cagaaatatg ggcctgaccc gcaccacgac cggggcgagt tgctgcccat cacgcccgag
     1621 cgggccaagg tggtcaacga gacgatctgg gcgctggtcc aacggcaccc gttcacgcat
     1681 ctcgctcaac attccgaagc agagaagcgt tgaaatagcg ggtgcgtgcc tgaatcgact
     1741 cccctcctgt tgtttccaac ggcaaagcat gtcacgcgat cagtcgggat ttgcgtcttt
     1801 tggttcccat agtgatgttg ctcgctgttt gaaaaaggga acattcctct ttgtgttagc
     1861 caaaacacgt ctggcgagag catgccagaa acgataccat aaacctatca aaggctgttg
     1921 tttgaggtgg tggtgaactg tgtcggtgcc tggaagctcg cggagtctgg acaatgacgt
     1981 ctttaacgcg cattcacact tgggcggctg tgtttatggt tggggtggtg tgtggagtac
     2041 tgctcagcac gcactggccg caaacaccga ttcgggcggt agccacggat cgtgggcaga
     2101 acttcgccgt ggccaccggg ccggtggacg ccggggtgga ggcctttttc ttcctggact
     2161 ttctcaccgg gcaactgcgg ggggtggtgc tctccaatca aacacggaat ttccaaacga
     2221 cttatgaggc caacgtgttt gccgacctga caacggtcat tcaattgaag aatgcagaga
     2281 ttcagaagct caacgctcaa gccgctcgaa gtgggggacc gacccggccg gagattcaaa
     2341 taccgcagtc accgaattat ttgttggtaa ccggcttggc cgatatccga cgtggtccgt
     2401 cagtgggtgt tcgaccggga caaaccatgg tttatgtcac ggagacgaac accggtatcg
     2461 ttctcgccta catggtgccc tggtcaccgg aacggcattc ggctaatcaa ccgttctcga
     2521 cacccctaca actgtgggca gccgagcaat tcagcacggt tgtgttgcga accgagtgat
     2581 tcggtgcttg tgtgcgatga tttgaaagcc cacgtcccgg ggttgatctc ggggcgtggc
     2641 tttgtcgtag tatgaggtta gggggcgcgt tgagattgaa tcacacaatg gtcgggtgtc
     2701 cctatggcaa atcatccgga ttctcagaca atccgaattt tgattaacgg cgagtggcgg
     2761 gaggtggctt ccggcatcac cttggccgat ctattgaagg aactcaaatt ggccgataag
     2821 cctgtggctg tggaaaggaa tcttcaggtt gtgcccaagg cgaggcaccc cgaaacaccg
     2881 ttggctgacg gagaccgact tgagattgta accctggtgg gtggaggttg attttggcat
     2941 ggccacgcat gattttgatt cgggccttcg gatcggctcg catgttttta aaagtcggct
     3001 gatcgttgga acggggaaat acagttcctt tgaggtgatg caacaagccc tggagctttc
     3061 ggggacggag tgcatcaccg tggccgtgcg gcgggaacga ctgttcgacc agcaagggcg
     3121 caacatcctc gatttcattg acaccaagcg atacaccatc ctccccaata cggcgggatg
     3181 ctttacggcg gaagacgcgg tgcgggtcgc ccgtttgggc cgggaaattc tgacccaatt
     3241 gggaaatcct ggggcggact gggtaaagct ggaggttctg ggcgacaaag gaacgcttct
     3301 tcccgatccg atcgccacgg tccaggccac cgagattttg gttaaggatg gctttcaagt
     3361 cctcgtttat accacggatg atccgattgt ggccaagcga ttgaaagaag cgggggcgac
     3421 gagtgtcatg ccggcgggaa gtcccatcgg ctccggccaa gggattctca acccaaacaa
     3481 tatcaaaatc tgtctggagt atttgaagga aaacgatccc gactatccgg tgatcgtgga
     3541 tgctggcgtg ggaacggcca gtgacgtaac ggtggctttt gagcttggcg ttgatggggt
     3601 ccttctcaat acgggtattg cccatgccaa ggacccactg cggatggccg tcgccatgaa
     3661 atacgctgcc gaggccggac gattggcgta cctggcaggc cgaatcccga aaaagctgta
     3721 cgcaacggcc agcagtccct tggaagggac gatcgctcca tcccatgagg cgggcaaata
     3781 ggggtttgat tgcaatttcg cctttacagg gagggacgcc gtcctccgcg tccgccgtgt
     3841 ttctccttag agcatatccc acaacctccc gtcacccgtt gagcgggaga ggagtcaagg
     3901 gtgagggtgc ctcgaatcgc tgccggtttt ctcaatcgat tccgacgttc gcttgccctc
     3961 acccc
//
LOCUS       DAOPTR010000254         3964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_18253_length_3964_cov_3.322589, whole genome shotgun sequence.
ACCESSION   DAOPTR010000254 DAOPTR010000000
VERSION     DAOPTR010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18253_length_3964_cov_3.322589"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(254..1627)
                     /locus_tag="PKI05_06080"
     CDS             complement(254..1627)
                     /locus_tag="PKI05_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOA51805.1"
                     /translation="MESQQLVPRNVPRVETRHRRIVTPIPVPESIPILEQLRRNEPRS
                     MSGQPPVVWYRAEGVYVFDRWGNQWLDWSSGVLVANAGHNHPKIQKAILDQVQHGLIH
                     NYCFPSEIRARCVEALARVAPPGLSKVFLLTTGAETLECAIKLCRTWGQHIGGRKKIG
                     IVSFHNAFHGRTLGAQMIGGIPALKKWIVNLDPDMIQVPFPDGFRGVDTSFEGFLRAL
                     DSQGYTPDRVAGVITETYQGGNASFAPAEYMKKLRAWCTEHQALLVMDEVQAGFGRTG
                     TYWGFEHYGIVPDLICCGKGISSGLPISAVIGRPEVLDLYPPGSMTSTHTGNPVCCAA
                     VLANLQVIEEEKLVERARQLGDILHAELRRLRVRFLDHIGMVHGRGLVASLHMVKPGT
                     IEPWAELASRVVMRCVEKGLMLFAPVGYAGASVKIAPPLVISEDALREGITVLEEALE
                     EVIQEIT"
     gene            complement(2455..3723)
                     /locus_tag="PKI05_06085"
     CDS             complement(2455..3723)
                     /locus_tag="PKI05_06085"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA51806.1"
                     /translation="MPSHVFGQTAPSEAVCLGIIGTGRQTFNVNIPQFQNQPDCRIVA
                     LCDVDRWRLEQAKARIDEFARKQYQITTGAATYVDYHDLLAREDIDAVLIATPDHWHG
                     VMAVDAMRAGKDVALEKPIIRTFSEGKRLAETAARLNRIFRVDSEFRVGAPARRAYSV
                     LRENLLGKILRVKVGVPQTDVACPPQPEMPVPEELDYARWQGAGVTQAPPIPYTLNGV
                     HPRHDLKGRPGWMRRLNYCDGMITNWGTHLLNGALWCLGLDRSWPTEIEGTGIYPPKD
                     SFWNVLLQFRVIYRFENGLEIEYHTERPYMRFEGEKGWIEADFNKFQASDDALVKKSF
                     PLTDSPPPRFTSEKRDFLDSVKSREPTWEPADVGHHVTSICLMGHIVVFLGEKLRWDP
                     DRESFVDNPEANKYLSEPIVAPNPRFQKPA"
BASE COUNT          911 a         1121 c         1016 g          916 t
ORIGIN      
        1 agacggcaag atctgatcat gtgtgatcat cccaccgcct acgatgacaa cttccagttt
       61 ttatgaaaac tctgacatct gttcggctcc ttgatcgttc aaagatctct tgcggttacc
      121 acctcattcg ttggctgatg gtgcccacga tatagcgcta aagagtagct cttcccatac
      181 gcttttgcga gtttctccta tatccccaaa cctatggcag taggggaact ttaccgaaga
      241 gatggcgatt gtcctacgtg atctcctgaa tcacttcttc cagggcttct tccaaaacag
      301 tgatgccttc gcgcagagcg tcttcggaaa tcaccaatgg cggggcgatt ttgaccgagg
      361 cccccgcata gccgactggt gcaaacagca ttagcccctt ctccacgcaa cgcatcacta
      421 cccgggaagc caattcggcc caaggttcaa tcgtccccgg cttgaccatg tggagggaag
      481 cgaccaaacc ccgaccatgg accattccga tatgatcgag aaaccggacg cgcagtcgtc
      541 gcaattccgc gtgcaagata tcacccagtt ggcgggctcg ttcaactagt ttttcttctt
      601 cgatcacttg cagatttgcc aacaccgcgg cgcaacaaac gggattcccc gtgtgcgtgg
      661 aggtcattga tcctggcgga taaaggtcca acacttccgg acggccaatg actgctgaaa
      721 tgggcaaccc gctggaaatc ccttttccgc aacaaatgag atccggaaca attccgtagt
      781 gctcaaatcc ccaataggtc ccggtgcgac cgaagcccgc ctggacttcg tccatgacca
      841 ataatgcttg atgttcggtg caccatgcgc ggagtttttt catatattcg gcgggggcga
      901 aactggcatt gccgccttgg taggtctccg taatcacgcc ggccacccga tcgggcgtat
      961 agccctgtga atcgagagcc ctgagaaatc cctcaaagga tgtgtccacc ccacgaaaac
     1021 cgtcggggaa agggacttga atcatgtcag ggtcgaggtt gacgatccac tttttcaagg
     1081 ctgggatgcc gccgatcatc tgggctccca gagttcgacc gtggaaagcg ttatggaatg
     1141 agacaatgcc gatctttttc cgacccccaa tgtgttgtcc ccatgtgcgg caaagcttga
     1201 tggcacattc cagggtttct gcaccggtgg ttaaaaggaa gaccttgctc aagccgggag
     1261 gtgcaacccg ggccaacgcc tcgacgcatc gggcccgaat ttccgaagga aaacagtaat
     1321 tatggatcag tccatgctgc acctggtcca aaatagcctt ttggattttg gggtgattgt
     1381 gcccagcatt cgcgaccaac acgcccgagg accagtccag ccattggttt ccccaacggt
     1441 cgaatacata caccccttct gcccgatacc acacgaccgg cggttggcca ctcatagatc
     1501 gaggctcatt acgacggagt tgttctaaga tcggaatcga ttccggtact ggaatgggag
     1561 tcacaatccg gcgatgccga gtctccacac gtggcacatt gcgaggcact agctgttggg
     1621 attccatagg tcctctcacc tctctttcga ttcactttac gagaacaaaa acactttacg
     1681 aaccctcaaa tctagtcagt tcttaacgcg atcttctggc aaccttttgg cttataagag
     1741 cctatcccat aatctcccct ctcccgttca gcgggagagg ggtcgggggt gagggtggct
     1801 cgaatcgctg ccggttttct cagtcgattc cgacgttcgc ttgccctcac cccagccctc
     1861 tcccagaggg agagggggac tttttgggat agactctacg gcggaatcgc tctgcggatc
     1921 tcgtcgccga ccgaggcccg acgtacctaa ggggccaaaa taaaaaaaca ctccacctca
     1981 gaccgggcct ggtatttcag gccaagcggt ccaagacgga gtgctctttt ctaggctgac
     2041 agtgccgcta cggatgccag cgagctacta aattgtcatt cggtgcgggc cgcacaaact
     2101 cgttcccccc gctagaaccg aaaatctaaa agcgcccatc gaagggggaa acttgccgca
     2161 ccgattgcct atattttaag aagctcacct ctgctttcaa gaccgggatg caaaagtggc
     2221 aagacctcat tcttcaaggc ttggacctct ggccgagaac cgcttaagag cctatcccat
     2281 aatccctgct cgcctgttgg gcgggagaaa agtcgggggt gagggtggct cgaaccgctg
     2341 ccggttttct caatcgattc gacgttcgct tgccctcacc ccaaccctct cccggaggga
     2401 gagggggagt ttttgggata gactctcatc ccccagtccg tagatcccga accgtcacgc
     2461 gggtttttgg aaacgtggat ttggtgctac gatcggctct gacagatact tgttggcctc
     2521 tggattgtcc acgaatgatt cgcggtccgg gtcccagcga agcttttcgc cgagaaagac
     2581 gacaatatga cccatcagac atattgacgt cacatgatgg ccgacatcgg ctggctccca
     2641 cgttggttca cgacttttaa cagaatccag gaagtcgcgt ttttcgctag tgaatcgggg
     2701 tggcggtgaa tcggtcaagg gaaatgattt cttcaccaag gcatcgtctg aggcttgaaa
     2761 tttgttaaaa tcggcttcaa tccagccttt ttcaccttca aaccgcatgt acgggcgctc
     2821 tgtgtgatat tcaatttcta aaccattctc aaaacggtag atcacccgaa actgtaacag
     2881 gacattccaa aagctgtcct ttggcggata gattcccgtt ccttcgattt cagttggcca
     2941 gctcctgtca agtccaagac accatagcgc cccgttgagc aagtgcgtgc cccaattggt
     3001 aatcatccca tcgcagtagt tgaggcgacg catccagccc gggcgaccct tgagatcgtg
     3061 tcgcgggtgg acaccgttta aggtgtacgg gatgggtggg gcctgagtca caccagcacc
     3121 ttgccagcga gcgtaatcga gctcctcggg tacaggcatt tcaggttggg gaggacacgc
     3181 aacatcggtt tgcggcacgc cgactttgac gcgaagaatc ttacccagca ggttctcgcg
     3241 cagaacagag tatgcccggc gtgcgggggc acccactcga aattcgctgt caacacgaaa
     3301 tatccgattc aagcgtgcgg cggtctcggc gagtcgtttt ccctccgaaa acgttcgaat
     3361 aatcggcttt tccaacgcca catctttgcc ggctctcatg gcgtcaacag ccatcacacc
     3421 atgccaatga tctggtgtgg caattaggac ggcatcgata tcctcccgtg caagcagatc
     3481 atgataatcc acgtaagtgg cagcacctgt tgtgatctga tattgctttc tggcgaattc
     3541 gtcgatgcgg gccttggcct gttccaaccg ccagcgatcg acatcgcaga gcgccacaat
     3601 ccggcaatcg ggttgattct gaaactgggg aatattcaca ttaaaagtct gtcggccagt
     3661 tccgataatt cccagacaaa ctgcctcgct gggtgccgtc tggccgaaaa cgtggctcgg
     3721 cacgaaagtg gggatcagtg gcaacgctgc tccggaccat tttaagaaga ctcgtcgcga
     3781 aacttttggt cgcttggctg ccataggttt cttctcccaa aaccaaacaa aatcgaattc
     3841 accaagcacg gtttttccaa catgcactac gactagggac agcggggcat aatctgcttc
     3901 caggccgcaa gtgttcgctc aagtagctcc tttgccggaa cggttaagcc gtagcactga
     3961 acgt
//
LOCUS       DAOPTR010000255         3958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_18284_length_3958_cov_3.684089, whole genome shotgun sequence.
ACCESSION   DAOPTR010000255 DAOPTR010000000
VERSION     DAOPTR010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18284_length_3958_cov_3.684089"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PKI05_06090"
     CDS             complement(<1..585)
                     /locus_tag="PKI05_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51807.1"
                     /translation="MKTVSQTKSRPTVSVEQRGFTDEYGNWVALKDPWTAAFLAWLLP
                     GLGHWYQGRWAKAILFFVCITGLFVYGCYLGSRRDLGYARVVYASFRPGDMRLYYLCQ
                     VGVGLPALPALVQSARVRQGKAPLWNYFMAPPRLGTDDGDPPTLSRLCYELHSYFDLG
                     SLYTAIAGLLNLLVVFDAFSGPIFPEDSARREEKA"
     gene            complement(782..1930)
                     /gene="nadA"
                     /locus_tag="PKI05_06095"
     CDS             complement(782..1930)
                     /gene="nadA"
                     /locus_tag="PKI05_06095"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOA51808.1"
                     /translation="MSTTLERQTAGALPFAAYKGLSQEELTARIQAVREKMGPRLLIL
                     GHHYQRDEVIELSDLQGDSYRLSALAAENKDCRAIIFCGVHFMAETADILANRPERVK
                     ERRGERIPVVLPDMKAGCSMADMAAIDQVENCWNQLGQVIDLSEVIPVTYVNSAASLK
                     AFCGRHGGTVCTSANADVALQWALSRGRRVLFFPDQHLGRNTALRMGIPLEEMPVWDP
                     QAEELGGQTKEAIQKSRVILWKGYCSVHQIFLPSHVDYYREHYPGIRILVHPECMREV
                     VEKADVVGSTGKIIRAVEEAPPGTKWAIGTELHLVNRLKKKHPEQEIYFLAPVVCMCS
                     TMFRIDLPHLCWAAENLAAGTPVNVIRVDDATAADALVALERMLQLKG"
     gene            complement(1962..2429)
                     /locus_tag="PKI05_06100"
     CDS             complement(1962..2429)
                     /locus_tag="PKI05_06100"
                     /inference="COORDINATES: protein motif:HMM:NF016102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HOA51809.1"
                     /translation="MAKIIVIIFILGITELATLLWLGSYAGWLLALGEILVTLLLGLG
                     IIRWVGVFSFTGTPRLPPIDGFLIVAPIDALVLLVAGVLLMLPGLVTDCLGLLLVFLP
                     TRRLLLRMALWYLGRKLFPSVFSQSDEFESPNPEGDIEVSSDEIINVEFYRIL"
     gene            complement(2773..>3958)
                     /locus_tag="PKI05_06105"
     CDS             complement(2773..>3958)
                     /locus_tag="PKI05_06105"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOA51810.1"
                     /translation="GEELPQEIRKPVTINAQNYPSLQAAFDAVSQNGGLLTIPAGDYV
                     ITEPLVVHGSEVRIVGRGPATRIINHNTEGKPAIIVRPADRETNPRARLWRVEFAYLR
                     IQGDPKAIDAKSTEPTSGPGLVAEGVNEILLHALSIDHNGGHGIALIDCYEDPRICDC
                     IITYNRGAGIFLKACHDIVVNANQLEENQQALVCIDSFNLCMNGNNLDDHLGEGIIVE
                     NTYGSVVSGNMIEECQGPAIVLDRDCYGITLSSNVLADNFGGAIDLRDAWGCTVSANT
                     FTIDPVFAVRIGPNSGRITITGNNFSDSYIGQQTRRTDKANLATGIVLEGTTDIVISG
                     NIFTGLADPAVTARGGCRRLAITGNIIRDVAQSRAEDRKALDLGDATDVVLANNILPE
                     AQ"
BASE COUNT          973 a         1128 c         1009 g          848 t
ORIGIN      
        1 ggccttttcc tcacgacggg cggaatcttc cgggaagatc ggcccggaga aggcgtcaaa
       61 aacgaccaac aggttgagca agccggcaat ggccgtgtaa aggctcccta aatcgaaata
      121 ggagtgaagc tcataacata agcgagaaag ggtcggcgga tcgccatcat ccgtgcccaa
      181 gcgcggtggc gccataaagt aattccataa cggggccttt ccctgccgga cacgtgccga
      241 ttgaacgagt gccggaagtg ccggaagtcc caccccaact tgacacaagt aataaagccg
      301 catgtcacca ggacgaaagg aggcatagac aacacgggca tagcccaagt cgcggcgact
      361 ccctaagtaa cacccgtaaa caaataagcc cgtaatacaa acaaaaaaga ggatggcctt
      421 ggcccaccgc ccttggtacc aatgccccag accagggagc agccaggcca agaatgcagc
      481 ggtccaaggg tctttcaaag ccacccagtt tccatattca tccgtaaaac cgcgctgctc
      541 aaccgaaacg gttggccgcg attttgtctg cgacactgtt ttcatgattg ttcttgctat
      601 ccactaaacc aggagaattc gcattgggca ggaagaaagg gaagaaggtc cagcgatcct
      661 taacctttga gactacccga cagggtgctt ttttgcaacc caagttgtca agtttcctaa
      721 cgttatgacc aagggcagaa cataccgcaa aagatacaag gcaataccac cttttgcgca
      781 tctagccctt gagttgcaac attcgttcca gggcaaccag agcgtcagca gccgttgcat
      841 catccacacg aatcacgttg accggcgtcc cggctgccaa attctctgcg gcccaacaaa
      901 ggtggggcag gtcgatccgg aacatggttg agcacatgca caccaccggg gcgaggaaat
      961 agatttcttg ttccgggtgc ttcttcttca ggcgattgac caggtggagc tctgtcccga
     1021 tggcccattt tgtgcccggt ggggcctcct ccacggcacg aataatcttt cccgtcgatc
     1081 ccactacgtc cgccttttcc acgacctccc gcatacattc gggatggacc aggatacgga
     1141 tgccagggta gtgctctcga taatagtcaa catgcgaggg caaaaagatt tggtgtacgc
     1201 tacaataccc cttccaaagg atgacacgac tcttttggat ggcctctttc gtttgtccgc
     1261 ccagttcctc ggcttgaggg tcccatacgg gcatttcttc caaggggatg cccatcctca
     1321 gcgctgtatt tcgccctaaa tgctgatccg ggaaaaacag cacccgtcgg ccacgcgata
     1381 gcgcccattg taaggctacg tcggcgttgg ccgaggtaca caccgtcccc ccatggcgcc
     1441 cacaaaatgc cttaagcgac gcggcagaat tgacgtaggt caccgggatc acctcgctta
     1501 ggtcgatgac ctgccccagt tggttccagc agttttcgac ttggtcgatc gcggccatgt
     1561 cggccattga gcaaccggcc ttcatgtcgg ggaggacgac gggaatcctt tcgccacgcc
     1621 gctctttgac gcgttccgga cgattggcca gaatgtcggc ggtttctgcc ataaaatgga
     1681 cgccacagaa gatgatcgcc cggcagtcct tgttttcggc tgccagggcg ctcaagcggt
     1741 aactgtctcc ctgcaggtca gatagttcaa tgacctcatc ccgctgataa tgatgcccca
     1801 ggatcaaaag tcgcgggccc attttttcac ggacggcctg aatacgggcc gttaattcct
     1861 cctgggaaag acctttatag gcagcgaacg gaagagctcc cgctgtttgg cgttccagtg
     1921 ttgtgctcat gaactctcca agctttccaa aaactcgatg actacaaaat acggtagaat
     1981 tcaacgttaa taatttcgtc ggagctcacc tcgatgtccc cctcgggatt aggagactca
     2041 aactcgtcgg actgagagaa cacgctagga aacagttttc ttcctaaata ccaaagcgcc
     2101 atgcgcagta gtaaccgccg cgtaggtaga aaaactagta gaaggcccag gcagtcagtg
     2161 actaagcctg gcaacatgag caatacacca gccactagca aaaccaaagc atcaatcggc
     2221 gcgacaatta gaaacccatc tattgggggc agtcgaggag tcccagtaaa ggaaaaaacg
     2281 cccacccaac ggatgatacc cagtcccagg aggagcgtca ccaggatttc cccgagtgca
     2341 agtaaccatc cggcgtaact tcctagccaa agaagggtgg ccaattcggt aataccgaga
     2401 atgaagataa taacaatgat cttcgccatc acccgaatat tccttttcag cctatcttgc
     2461 caagggccaa tagcaaggcc ccacttgtgg cggaacgttt catcaccgcc cggtagctgc
     2521 cttgatttat tctaccgctt tgaataaggc cgggccacga gcacccgcta attggtaccg
     2581 gcggctagta tacaaattct gcacacaggt gaataccacc aagttgaacc aggcgcaatc
     2641 acaggagaca cgcggaccag cgaatctcct gtccccacag gcccttacac atcacagggc
     2701 agaagatcgc agacgttgac gatacctcca agcccgttag gggcaaagcc tcccttcgct
     2761 atttcgccga gcctattgcg cttccggtaa gatgttatta gcgagaacga catctgtggc
     2821 gtcacccaga tcaagggcct ttcgatcctc cgcccgcgac tgagcgacgt cgcgaataat
     2881 atttcccgtg atcgccagac gacgacaacc accgcgggcg gtaaccgccg ggtcggccaa
     2941 gcccgtaaaa atattgcccg aaatcacgat atccgttgtt ccctcaagga caatgccggt
     3001 ggccaggttt gccttatctg tgcggcgagt ttgctgaccg atatagctgt cggagaaatt
     3061 gtttcccgta atcgtaatcc gcccactgtt gggaccaatt ctgaccgcaa aaaccggatc
     3121 aatggtgaac gtgtttgcgg acaccgtgca gccccatgcg tcgcggagat caatcgcccc
     3181 tccgaaatta tctgcaagga cgttggaact caacgtaata ccgtagcaat cccggtccaa
     3241 gacaatggcc ggtccctggc attcctcgat catatttccc gagacgaccg aaccatacgt
     3301 gttttcgacg ataatcccct cgcccaaatg gtcgtcgagg ttgttgccgt tcatacaaag
     3361 gttgaagcta tcgatacata caagggcctg ctggttttcc tccaattgat tggcattgac
     3421 aacgatgtcg tgacacgcct taagaaagat gcccgcacct ctgttgtacg tgataatgca
     3481 gtcgcagatc ctagggtctt cgtagcaatc gatcaaggca atcccatgac cgccgttgtg
     3541 atcgatgctc agtgcgtgaa ggaggatttc attgacccct tcggcaacca gccctggccc
     3601 actggtcggt tcggtacttt ttgcgtcgat cgccttagga tcgccttgga ttcgcaaata
     3661 cgcaaactca acccgccaaa gccttgcccg cggattggtc tcacgatcgg cgggtcgaac
     3721 gatgattgca ggcttgcctt ctgtgttgtg gttaatgatt cgggtggcag ggcccctgcc
     3781 aacgatgcgg acctccgaac catggacgac aagcggttct gttatgacgt agtcacccgc
     3841 tgggattgtg agaagcccgc cattttgcga aacggcgtcg aaggccgcct gaagactggg
     3901 gtagttctga gcattgatcg taacgggttt gcggatttcc tggggtaatt cctctcct
//
LOCUS       DAOPTR010000256         3943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_18358_length_3943_cov_3.702932, whole genome shotgun sequence.
ACCESSION   DAOPTR010000256 DAOPTR010000000
VERSION     DAOPTR010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18358_length_3943_cov_3.702932"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            285..1718
                     /locus_tag="PKI05_06110"
     CDS             285..1718
                     /locus_tag="PKI05_06110"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA51811.1"
                     /translation="MNSVTRWLPRVGLAAALGLLSGMSLLASETAQRPNVLIITTDQQ
                     RVDAASVVGNKWLKTPHLDALAAQGVYFTKSYCSHPLCSPSRASLHAGRFPHEIGVDH
                     NNMSITPSVTISGQVFQEAGYDTGYAGKWHIPTVYPTDGIPGFTVLNNTTRQGKLAHD
                     VDDATMQVAIDFIKQKRDKPFYLVVSFINPHDICLLAGEDSPLLEQVWRKYGPPAGAE
                     LPPLPASFTLPENEPAWLLQRRPKHPQWDETHWRRYIYAYYRMVEDVDQQIGAVLKAL
                     KETGQEENTLVIFTSDHGEGLGSRRWTGKMMFFDPEAAVPLIVSWKGKTPAGRIDKEH
                     LVSTVDVLPTICDYAGIKPPENVRGQSLRPIIENPAAPWRSFIVSEIAHTPALGRSFM
                     VRTDRYKYFVIPVGQEKPLEMLFDMETDPHELKNLASDPNHSETLKEHRALLDQWKQT
                     TEEAKYPVLPNPQAARPRAQRAKQAAP"
     gene            1863..3428
                     /locus_tag="PKI05_06115"
     CDS             1863..3428
                     /locus_tag="PKI05_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA51812.1"
                     /translation="MKPLFRGQQGPTVFWASMLVAIGCSYQWGQAGWATEKRPNILLI
                     EVDSMDGRVMGCMGYPAMVRATPNLDALAKRGVLFRNAYTNNPICCPSRASMWSGKYT
                     FRIEAWNNYKGLEPGTPTFRDELEKAGYLTQTFGKLDYLSGAHSVRARVTAWLRSANI
                     ERPQYRIGPPRVIEEDLVRVHAGDWRNADRAVAWLKDNAKGDKPFWLYLGLSLPHPAF
                     QTSRYYLRMIDEAGVDVPPLDQYDHSVMRYQRLVKNWEHGYSDEMIKLVRRVYFAMIA
                     ETDAIVGHVLQALRDCGLEDSTYVIFISDHGENAMEHRLWYKMNHYESSARVPMIIAG
                     PGVRQGVVEEKLVSLIDIYPTLMDMANWPKPQDLDGHSLMPELLGQPSSRPDWVLSEY
                     HDTACNTGSFMIRRGPWKYIAYPGYEPMLFNLDEDPHEIRNLAPQLPDKVLEMDQLLR
                     SIVDYEAVDAKAKAYDRASFAAWREEHKQKGDYEQLMAIIFSGYDGVNAAKAKPWTPE
                     DEAKVERWLKGEQ"
     gene            3462..>3943
                     /locus_tag="PKI05_06120"
     CDS             3462..>3943
                     /locus_tag="PKI05_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51813.1"
                     /translation="MTSEKNGLVATVRRLVPWRLLFWIALCSVGPLCLSGQIGFGAEQ
                     GSAGDPVEIIEAMVDAQATIRYLLAHQRENGAFGPMGHQHTDLAWNYPAARALILLEV
                     EIPRVEDCFRNGQDALYKHPGSHNTQFAWDIYQRAQLAQCLGHRGEDGLGLAGAWKLQ
                     "
BASE COUNT          889 a         1049 c         1131 g          874 t
ORIGIN      
        1 gatcgtcatt ctccatgtcc cccattttag cttaagacca cttatcgggc tacgatgatg
       61 cggcaactct ttctccctga ggatgcccat ttgcccgtcc gtaggcggct cctagaggtc
      121 gcaggaattg actctgtacc gatccgtatt aaattgaagg ggcagagcgt ggacagtttc
      181 ctggcgtggc atcgcatgtc gtagtgtgct gcggaaactg tttatccgct ggcgacgtgt
      241 ttcgatggtc atacagtatt tcttttcgtg gggaggtttc agccatgaac tcggtcacac
      301 gttggcttcc gcgcgttggc ttggccgcgg ccttgggact gttatcgggt atgtcgttac
      361 ttgcatctga aaccgcgcag cggccaaatg tgctgattat cacaaccgat cagcaacgtg
      421 ttgacgcggc aagcgtggtg ggaaacaaat ggctcaagac gcctcatcta gatgccctcg
      481 cagctcaagg tgtttacttc acaaagtctt attgttcgca tccgttgtgt agtccgtcgc
      541 gggcgagcct tcacgcaggg cgatttcccc acgaaattgg cgtggaccac aacaatatgt
      601 caataactcc gtccgtcacg atttccggtc aggtttttca agaggcgggc tacgacacag
      661 gttacgccgg taagtggcat ataccgacgg tttacccgac cgatggcatt ccgggtttta
      721 ccgtgctgaa taataccacg cggcagggaa aactagcgca tgacgtggat gacgcgacaa
      781 tgcaggttgc catcgacttc atcaagcaga aacgggataa accgttttat ctggttgttt
      841 cgtttatcaa tcctcatgac atttgtctgc tggcgggcga ggatagcccc ctccttgagc
      901 aggtttggcg gaagtacggt cccccggcgg gagccgagct tccaccgctt cccgcaagtt
      961 ttaccctccc agaaaatgaa ccagcgtggt tgctccagcg acgtcccaaa catcctcaat
     1021 gggatgaaac gcactggcgg cgatatattt acgcctatta tcgtatggtc gaggatgttg
     1081 atcagcagat cggagccgtc ctaaaggcac tgaaggaaac tgggcaagaa gagaacacgc
     1141 tggtcatctt cacaagcgac cacggggagg gcttgggttc ccgtcgctgg acgggcaaga
     1201 tgatgttctt tgacccagaa gcggctgtgc ccctcatcgt cagttggaag gggaagaccc
     1261 ccgcgggccg gatcgacaag gagcatttgg tctccaccgt cgatgtcctt ccaacgatct
     1321 gtgattatgc gggaatcaaa ccgccggaga acgtccgtgg acaaagcctc cgaccaatca
     1381 ttgagaatcc tgccgcacca tggcggtcgt ttattgtctc ggagattgcc cacacaccgg
     1441 cgttgggacg gagcttcatg gtgcgaacgg accgctacaa atattttgtg atccctgttg
     1501 ggcaggagaa gccgttggag atgctcttcg acatggaaac agatcctcac gaattgaaga
     1561 acttggcttc cgatccgaac cactccgaga cgctcaaaga gcaccgcgcc ttgctcgacc
     1621 aatggaagca aaccacagaa gaagcgaagt atcccgtcct acccaacccc caagcggcac
     1681 gccccagggc acaacgagcc aagcaggctg caccataaga gggtgtttgt caccgtcaag
     1741 cgatacgggc acgcaacggc cacgttgtgc ggcgcatggc gttggacgag ctgatttggt
     1801 aacgtgaagc catcttaacg atgcttggct tagcgaatct gttcatgtta ggagtcaaaa
     1861 tcatgaagcc gttgttccgc ggtcaacaag ggccaactgt gttttgggcg tcaatgttgg
     1921 ttgcaattgg ttgttcttat caatgggggc aagcgggatg ggctacggag aaacggccaa
     1981 acatcctcct tatcgaggtg gatagcatgg atggccgagt catgggatgc atgggttatc
     2041 ccgccatggt gcgggcaacg ccaaacctcg atgccttggc caaacggggg gttcttttcc
     2101 gcaatgcgta caccaacaac cccatttgct gtccttcgcg ggccagcatg tggagtggta
     2161 aatacacgtt tcgcatcgag gcctggaata attacaaggg gcttgaaccc gggacgccga
     2221 cgttccgcga cgaattggaa aaggcgggct atctgacaca aaccttcggg aaattggatt
     2281 acctgtccgg cgcccattcg gtgcgggcca gggtgacggc gtggcttcgc tcggccaaca
     2341 tcgaacggcc acagtatcgc atcgggccgc cgcgggtcat tgaggaagac ctcgtgcggg
     2401 ttcacgccgg agactggcga aatgcggatc gcgcggtggc ctggctaaag gacaacgcaa
     2461 aaggcgataa gcccttttgg ttgtacttag gcttgagttt gcctcaccct gcctttcaaa
     2521 cgtctcgtta ttatctgcgg atgattgatg aggcgggagt cgatgtgcct cccctcgatc
     2581 aatatgacca ttccgtcatg cgctaccagc ggttagtcaa aaactgggag catggttact
     2641 ccgacgagat gatcaagctc gtccggaggg tctatttcgc catgatcgcc gagactgacg
     2701 ccattgtcgg gcacgtgctg caagccctcc gtgactgcgg tctggaagac tcgacctacg
     2761 tgatcttcat cagtgatcat ggcgagaacg cgatggaaca ccgactctgg tacaaaatga
     2821 atcactatga gtcgtccgct cgtgtcccca tgattatcgc cggacccggt gtgagacaag
     2881 gcgtcgtgga agaaaagctg gtttcgctga ttgacattta cccgacgtta atggacatgg
     2941 ccaattggcc caagccgcag gacctggatg ggcattccct catgccggag ctattgggcc
     3001 agcccagctc ccggccggac tgggtattga gcgaatacca cgacaccgca tgcaacacag
     3061 gcagcttcat gatccgccgt ggtccctgga agtacatcgc ctatcccggt tatgaaccaa
     3121 tgctcttcaa ccttgatgaa gacccccatg agattcggaa tctcgcgccg caattgcctg
     3181 acaaggtgct tgagatggac caactgctgc gctccatcgt ggattacgag gctgtcgatg
     3241 ccaaggccaa ggcatacgac cgtgctagtt ttgccgcgtg gcgtgaggaa cataagcaga
     3301 agggtgacta cgagcagctc atggccataa tcttcagcgg ttacgacggt gtgaacgccg
     3361 cgaaagccaa gccctggacg cccgaggatg aggccaaagt cgaacgctgg ctcaaaggcg
     3421 aacaatgatc ataagtgacg aaatcgtgcg ggaatatcat tatgactagc gaaaagaatg
     3481 gattagttgc cacagtgcgg cgccttgtgc cgtggcgctt gctcttttgg atagctcttt
     3541 gcagtgtggg gcctctctgc ctgtctggac agatcggttt cggtgccgaa caaggttccg
     3601 cgggtgaccc ggtggaaatc atcgaggcga tggttgatgc ccaagcaacc atccgctatt
     3661 tgctcgcgca tcaaagggaa aacggtgcct ttgggccgat gggacatcag cacaccgacc
     3721 tggcatggaa ttaccccgcc gcgcgtgctt tgatcctgct ggaggttgaa atcccgcggg
     3781 tggaggattg tttccgcaat ggacaggatg ccctttacaa acaccctggc tcacataata
     3841 cccaatttgc ctgggatatc tatcaacgtg cgcaactggc acaatgcttg ggtcatcgtg
     3901 gggaagacgg actgggcctg gccggggcgt ggaagcttca ata
//
LOCUS       DAOPTR010000257         3941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_18373_length_3941_cov_4.622748, whole genome shotgun sequence.
ACCESSION   DAOPTR010000257 DAOPTR010000000
VERSION     DAOPTR010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18373_length_3941_cov_4.622748"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PKI05_06125"
     CDS             <1..205
                     /locus_tag="PKI05_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HOA51814.1"
                     /translation="QLVTVGIGLDDWEWGVTLWARNPQFLKDIVYRMRFDEASARYAE
                     FGPFYSGYQATPQQILSQCRVCR"
     gene            296..1216
                     /locus_tag="PKI05_06130"
     CDS             296..1216
                     /locus_tag="PKI05_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA51815.1"
                     /translation="MLLRFLIDRLIWLAITLWVVFTVSFFLMRAVPGGPLDRERRLDP
                     QIEKNLAARYHLDEPVWQQYLRELGHVVQFDLGYSYRMADFTVNEVIAQGLPISAALG
                     LAAMGLALTLGFAAGVASAAFRNSVLDRGLMVLATVGLAVPNFILAGVLIMLLVFVLP
                     CFPAAGWGRPSHLVLPAVCLGLPYAASIARLTRTGLLEVLTQDYIRTARAKGLNQVTI
                     IFKHALPGALAPVVSFLGPAVAGIITGSPVVEKIFAIPGLGMHFVEAALQRDYTLSMG
                     LVLLFTLLLYGANVLVDLAYTILDPRVELQ"
     gene            complement(1231..2268)
                     /locus_tag="PKI05_06135"
     CDS             complement(1231..2268)
                     /locus_tag="PKI05_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOA51816.1"
                     /translation="MAPAVKPIPLNRAIAPFFVGVDLGGTSTKIGLVDDLGRPVCQPQ
                     EFPTAVEDVPEQLVARVAEVINQMIQTSGLESTEIARVGLGCPGILDLHTGVMVNPTN
                     FPGWGGFPIREALADRSGMPVVMANDASAAAYGELWIGSGRGFRSLVLLTLGTGVGCG
                     AIIGDVIIEGEHGHGTECGHILIDPSDTAPVCSCGQRGHLEAFASATAVVRRAREKIA
                     AGEAPLLAEKVSNGEKLSPLLLAKLAEAGDQPSLDLILETARYLGLGIVTLLHTFDPS
                     GLLLGGAMTFGGDKSPVGRRFLERIREEVRRRAFAALANKVAIRFAALGGDAGFIGAA
                     GIARLHHRRYR"
     gene            complement(2460..3494)
                     /locus_tag="PKI05_06140"
     CDS             complement(2460..3494)
                     /locus_tag="PKI05_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51817.1"
                     /translation="MKNTERDGQINAGYDSFLDTVTNIVGILIILVMVVGVRAQHLPF
                     TLKPDTSHAESAEEQARRARAEELARRSTALLSLRRDILELSATVEMVEAERLARERE
                     RQRLALAAALLKKELDEKTVALSQEERRREQLAEAIAACERDIAASQQRLAELSSTTE
                     QVQIVNHPTPLSVPVDFEEIHFVIRQGKIAYIPLSSLLDELMSDARRKIPRLFEVPEI
                     TGTVGPVGGFKLEYSIVRRTIGEAEIAGVPGAGAYAQLRRWILIPEGNVGEPVAQALE
                     ESSEFRRRIAGYRPDRTTVTIWCYPDGFADFRRLKDFLHERGYSVAGRPLPDGFPITG
                     SPGGTKSSAQ"
     gene            complement(3561..>3941)
                     /locus_tag="PKI05_06145"
     CDS             complement(3561..>3941)
                     /locus_tag="PKI05_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51818.1"
                     /translation="VEITTGVTLRPLAEQRGRNWGLPPHAASAIPVTRPIRIDCFPDR
                     VVLIPEQGLDHIRVFYLTPSPAKAVDELVKAIREYVETWGIAGNNMYWRPVLSVRVAP
                     GAEAQFQILQILLKDSGLTVEQTR"
BASE COUNT          747 a         1067 c         1276 g          851 t
ORIGIN      
        1 ccaattggtg acggtgggca tcgggttgga cgactgggaa tggggagtaa cgctttgggc
       61 caggaatcca cagtttttga aggatatcgt ctatcgcatg cggtttgacg aggccagcgc
      121 ccgttacgct gagttcggac cgttctacag cggctatcag gccacaccgc agcagattct
      181 ctctcaatgt cgggtttgcc gttgattgct agcggcagcg caggacaata ggaagtagtt
      241 aagggaacca caacctgtcg tctgtcggcg cctgaggcat tggggtgaac cgaggatgtt
      301 actccggttt ttgattgatc gcttgatctg gttggccatc acgctgtggg tggtcttcac
      361 cgtaagcttt tttctgatgc gggcagtgcc ggggggaccg ttggatcggg aacgtcgtct
      421 ggatccgcaa atcgaaaaga atttggcggc gcgctaccat ttggatgagc cggtttggca
      481 gcagtatttg cgagaacttg gccacgtcgt gcaattcgac ttgggctata gctaccgcat
      541 ggcggacttc acggtcaacg aagtcattgc ccaagggtta cctatttcgg cggccttagg
      601 gttagcggcc atgggattgg cgctgacatt gggtttcgcg gcgggggttg cctcagcggc
      661 ctttcgcaat agtgtcctgg atcgtggcct gatggtctta gccacggtgg gactggccgt
      721 tcccaatttc attttggcgg gcgtgctcat catgcttttg gtgtttgtgc taccgtgttt
      781 tccggcggcg ggttggggcc gaccgtcgca cttggtgttg cccgccgtgt gcttgggctt
      841 gccctatgcc gcgtcgatag cccgcttgac gcgcacggga ctattggagg tcctcacgca
      901 ggattacatc cgcacagcgc gggccaaggg gctgaatcag gtgacgatca tttttaagca
      961 tgccctgccg ggggcgttgg caccggtggt ctctttcctc gggccggctg tggctggaat
     1021 tatcaccggt tcgccggtgg tggagaagat ctttgccatc cctgggttgg ggatgcattt
     1081 tgtggaagcg gctcttcagc gagactatac gctttctatg ggattggttc tcctctttac
     1141 gctgttgctt tatggggcca acgtcctggt cgatttggcc tacaccattc tcgacccgcg
     1201 tgtcgaattg cagtaatcct tggggatgat tcatcggtaa cgtcggtggt gcaatcgggc
     1261 gatgccggcc gccccgatga agccggcgtc tccgcccagc gcggcgaagc ggatggcaac
     1321 cttgtttgcc agggcggcaa aggcccggcg gcgaacttct tcccgaatgc gttccaggaa
     1381 gcggcgtccg acgggagact tgtcgccacc aaacgtcatc gcaccaccca agaggaggcc
     1441 tgatgggtca aatgtgtgga ggagcgtgac gatccccaag ccgaggtagc gggcggtttc
     1501 aaggatcaga tcgagcgacg gttggtcgcc tgcctcggcc aacttggcca gcaaaagggg
     1561 cgacagtttt tcgccgttgg acaccttttc ggcaaggagg ggagcctctc cggcggcaat
     1621 tttttctcgt gcacggcgaa cgacggccgt ggcactggcg aaggcctcga ggtgtccccg
     1681 ttggccgcag gaacagacgg gggctgtgtc ggagggatcg atcaggatat gaccgcattc
     1741 cgtaccgtgg ccgtgttcac cctcaatgat gacatcgccg ataatagcac cgcagccgac
     1801 gccagtcccc agcgtcagga ggacgaggct gcggaagccg cggccagagc cgatccacag
     1861 ttcgccgtac gcagcggcgg aggcatcgtt ggccatgacg acgggcatcc ccgaacgatc
     1921 agcaagggcc tcgcggatgg gaaatccacc ccaaccgggg aaattggtgg ggttgaccat
     1981 cacgcctgtg tgaaggtcca aaatacccgg gcaacctagt ccgacacgag cgatttctgt
     2041 ggattccagt ccggaggtct ggatcatctg gttgatgacc tcggccacac gggcgacaag
     2101 ctgttccgga acatcttcga cagcggtggg aaactcctgg ggctggcaca ccggacgacc
     2161 caggtcatcg accaggccga tcttggtgct ggtgccgcct aaatccacac caacaaagaa
     2221 aggggcgatc gcccgattga gcgggatcgg tttgacagcc ggcgccatga gaccctccgc
     2281 ataggattga cttgaggcac ttcgccaatt ggatcactat ctaggaaaaa taaaaggccc
     2341 gaattcgtca agcgcaaagc ctgaacgacg gggaatccca ccggttgacc gccggtggcc
     2401 cacgtcccgg agtggaatcg taacgattat tgcagttatc cggaatggct tcggaccacc
     2461 tattgtgcgg aggatttggt cccgccgggc gagcctgtga tggggaagcc gtcgggcagg
     2521 ggccggcctg cgacggaata gccccgctcg tggagaaaat ctttgagacg gcggaagtcg
     2581 gcgaagccgt cgggatagca ccagatggtg acggtcgtcc tgtccggacg atatccggcg
     2641 atacgccgac ggaattcgga tgattcttct agggcttggg cgacgggttc accgacgttg
     2701 ccttccggga tgagaatcca gcgtcgcaat tgggcgtatg ccccggcacc gggaacaccg
     2761 gcgatctcgg cctcgccgat ggtccgacgg acgatggagt actccagttt gaatccgccg
     2821 accggtccca cggtcccggt gatttcgggg acctcgaaga ggcgggggat ttttcggcgg
     2881 gcgtccgaca tcagctcatc gaggagcgat gagagcggaa tgtacgcgat ttttccctgc
     2941 cgtatgacga agtgaatctc ctcgaaatcg acggggacgc tcagcggcgt ggggtgattg
     3001 acaatctgca cctgttcggt tgtggaggac aactcggcaa ggcgctgttg ggatgcggcg
     3061 atatctcgct cacatgcagc gatggcctca gccaattgtt cacggcgtcg ctcttcctgc
     3121 gagagggcga cagtcttttc gtccagttcc tttttcaaaa gggcggcggc cagggccagt
     3181 cgctggcgct cacgttcgcg ggcgagccgt tctgcctcca ccatttcgac agttgccgaa
     3241 agttcaagga tatcgcgccg gagggaaaga agggccgtcg atcgccgggc aagctcttcg
     3301 gcgcgggccc gccgggcctg ttcttcggcg gattctgcgt gggacgtgtc cggcttcagg
     3361 gtgaagggca ggtgctgcgc gcggacgccg acgaccatca ccaaaataat cagaatcccg
     3421 acaatgttgg tgacggtgtc gagaaaggaa tcgtagccgg cgttgatctg tccgtctcgt
     3481 tccgtgtttt tcatcgtgga aaaagttttc ctggacaata cgccatattc attgatcgca
     3541 caatcgtgct cgctcgatcg ttatctggtt tgctccacgg ttagcccact gtcttttaaa
     3601 aggatttgca gtatttggaa ttgggcctcg gcccctgggg cgacacggac ggaaaggacc
     3661 ggtcgccagt acatgttgtt gccagcgata ccccatgttt ccacgtactc ccgaatggcc
     3721 ttaactagtt catccaccgc ttttgccgga gacggggtca ggtagaagac tcggatatgg
     3781 tcgagacctt gttccgggat aaggacaact cggtcgggga aacagtcaat gcggatggga
     3841 cgggtcaccg ggatggccga agcggcatgg gggggaaggc cccagtttcg gccccgttgt
     3901 tccgccaacg ggcgtagagt gacgcccgtc gtgatctcca c
//
LOCUS       DAOPTR010000258         3939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_18387_length_3939_cov_3.026519, whole genome shotgun sequence.
ACCESSION   DAOPTR010000258 DAOPTR010000000
VERSION     DAOPTR010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18387_length_3939_cov_3.026519"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="PKI05_06150"
     CDS             complement(<1..821)
                     /locus_tag="PKI05_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA51819.1"
                     /translation="MNPQRQVSVGVNSGARLQLDLLKTKASMVVAVVLGATLWWFLSG
                     SAKAAQPVGNQPTYSSPSPLAVIDRGGNGANQSAIASIHHVATATRDASKSRHPNIIL
                     ILADDLGYGELGCYGQQKIKTPNLDRMAAEGMRFTQFYAGAPVCAPSRCVLMTGKHLG
                     HAAIRDNSEVQPEGQWPLPAEEITIAEILKGCGYATAAIGKWGLGPPGSSGDPNAQGF
                     DLFFGYNCQRHAHNHYPTWLYRNREKVALRNPEFPAHQRFPEDADPNDPSAYARY"
     gene            complement(848..2875)
                     /locus_tag="PKI05_06155"
     CDS             complement(848..2875)
                     /locus_tag="PKI05_06155"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOA51820.1"
                     /translation="MNMRMWPWVMVLTAGCLVLGFHRCLAAESTGAVSPNEQAIQEVE
                     AGQRDTANAAWWGFYPEDSTQCLQAALRCKAKRVIIPKMASPWVVDKLELIGNKEIIF
                     EPGVEVVAKRGAFRGKGDCLLTAVNQENLTLVGNGATLRMWRDDYAQPPYEKAEWRHC
                     LSLRGCRNVIIDGLKLCESGGDGIYLGAGKNGEPNEKIVIRNVVCDRNYRQGISVITA
                     RDLLIENVVLSNTAGTPPEAGIDFEPNRATEVLINCVMRNCLIENNRGYAIHLYAGAM
                     NTESQPISIRLENCRTKGTNAGSLSIVTRNRAGEAVRGFFEVSTCHFEDSENAGVIIR
                     SKPADGFQVRLQNCEIVQLTENPKWPAPVTFVSRPGDENDVGGVVLDNLVVRENTDRP
                     LIHFNKGGGVCLKNVVGTATIYRGADRKTVSVDEDLILQWYPCSPIRQLPIVATDRLA
                     VWPTPSTQESLLDVPRHRLRQIGNYVFYAKKGDHVTFEMAYDQVGKNPGGRLPVRVFG
                     PAGNTVHTSELRFQEQTRLEFTIPEMGVYRVRAEPGPNAVRLVRTSHPVAILGERGII
                     HLIGTTGRFYLLVPPGKSLGLEVSGDPPGECVAACLFEGTSQPLWKITGCEDAKACLV
                     DPATVERVLCLELARPSQGVLEDEYIVIRGVPAVLSCWPDVLFVDSGEGRE"
     gene            complement(3133..>3939)
                     /locus_tag="PKI05_06160"
     CDS             complement(3133..>3939)
                     /locus_tag="PKI05_06160"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51821.1"
                     /translation="SGPRQPPQDTPPVPSGLDWDAWLGPAAERPYHPQYVSGWGRWWD
                     FGTGCLGGGGSHSLHLTFKALGLRQLWNPPHNSEKRIRVESEIPEKAPHGFPRWQFVR
                     FYIPARGDQPPATIYWYNADEAELHRRGVWKKLEDIAGRDLEWKDNSWTPRSGTLIVG
                     EKGVVHTNAHNSVCQILPADKFPQQAGRPKRYPHVAGHEYEWLNACRGQGSCLSQFDH
                     SGPVIELLLVANVAGRFDEPIEFDPVECRIIGNAEADALLRPPYRTGWSL"
BASE COUNT          877 a         1183 c          997 g          882 t
ORIGIN      
        1 atgtatcgtg cgtacgcgct gggatcgttc ggatcagcat cttccggaaa acgctggtgc
       61 gcgggaaatt ccggattgcg aagggcaacc ttctcccgat ttcgataaag ccacgtcgga
      121 taatgattgt gtgcgtgacg ctggcaattg tagccaaaga aaaggtcaaa tccctgtgcg
      181 ttgggatcac cgcttgagcc gggaggtcca agtccccact tgccaatggc agccgtcgca
      241 tagccacaac cttttaggat ttccgcaatg gtgatctcct cggcgggcaa cggccattgc
      301 ccctcgggct ggacttccga attatcacga atcgcagcat gtccaagatg tttgcctgtc
      361 atgagcacac aacgcgaagg cgcacaaacc ggtgcaccgg cgtaaaactg ggtaaaccgc
      421 attccctccg cagccatacg atcgagatta ggggttttga tcttctgctg tccgtaacaa
      481 cctaactcac cgtatccgag gtcatccgcg agaataagga tgatattggg atgacgtgac
      541 ttcgatgcgt cgcgcgttgc cgtagctacg tgatgaatac tggcgatcgc cgactggttc
      601 gcgccgtttc cgcccctgtc aatgaccgcc aaaggtgacg gactcgagta cgtaggctga
      661 tttcctacgg gctgggcagc ctttgctgaa cctgacagga accaccacag agtagctccg
      721 aggacaactg ctacgaccat cgacgccttt gtcttaagga ggtccaattg tagccgggca
      781 cctgagttta ctcccacgct gacttgcctt tgcgggttca tgataggctc ccttccctct
      841 tttttcgtta ctcacgtcct tcacccgaat cgacaaaaag cacgtcgggc cagcaactta
      901 agacggccgg gacaccccga atcacgatgt attcatcttc aagcacaccc tgcgacggtc
      961 gggcaagttc caaacagaga acacgctcca cagtcgctgg gtccaccagg catgctttgg
     1021 catcctcgca tccggtgatt ttccacaagg gttgcgaggt cccttcgaac aggcaggcgg
     1081 ccacacattc ccccggcgga tctccgctca cttcaagccc taatgacttg cccggcggca
     1141 caaggagata aaaccggcct gtggtgccga tcaaatggat aatccccctt tctccaagga
     1201 tggccacagg atggctggtt cgcaccaatc ggacggcatt aggccctggt tccgcccgca
     1261 cccgataaac acccatttcc ggtatggtaa actccagcct tgtctgctcc tgaaagcgga
     1321 gctctgacgt atgcactgta ttaccggccg ggccaaaaac ccgcaccgga agccgtcccc
     1381 caggattctt gccaacttgg tcataggcca tttcaaaggt cacatggtcc ccttttttgg
     1441 cataaaacac gtaattccca atctgccgaa gcctatgcct gggtacatcc agcagcgact
     1501 cttgcgtgga gggcgttggc cagaccgcga gtcggtccgt cgccacgatg ggcaactgac
     1561 gaatcggact acagggatac cattgcaaga taagatcctc gtcgacgctc acggtctttc
     1621 tgtctgctcc gcggtaaatg gtggccgttc caacgacatt ctttaggcag actccaccac
     1681 ctttgttgaa atggatcagc ggacggtcag tgttctcacg aaccacgagg ttgtctagca
     1741 caactccccc gacatcgttc tcatcccccg ggcgagaaac aaaagtgacc ggcgccggcc
     1801 attttgggtt ttctgttaac tgcacgattt cacaattctg gaggcggacc tgaaagccgt
     1861 cggccggttt agagcgaatt atgacgcccg cattctcgga atcttcaaaa tgacaagtgc
     1921 tgacttcgaa aaaaccacga actgcttcgc ccgccctatt gcgagtgaca atgctcaacg
     1981 acccagcgtt cgtgcccttt gtgcggcaat tttccagccg gatcgaaata ggctgcgatt
     2041 cggtattcat tgcgcctgca tacaaatgaa tggcataccc ccggttgttt tcaatcaagc
     2101 agtttctcat cacacagttg atgaggactt ccgtggcgcg atttggttcg aaatcaatac
     2161 ctgcctccgg tggtgtcccg gcggtattgc tgagaacgac gttttcgatg agcaaatccc
     2221 gggccgtaat gacgctaatc ccttggcgat agttcctatc gcaaacgaca tttcggataa
     2281 cgatcttctc gttgggttcg ccattcttcc ccgcgccgag atagatgccg tcaccgccgc
     2341 tctcacacaa tttgagaccg tcgataatca cattccgaca tcccctgagg ctcaagcagt
     2401 gccgccactc cgctttttcg tagggcggtt gggcgtaatc atcccgccac attcgcagcg
     2461 tggccccatt tcctaccagc gtgaggtttt cttgattgac tgctgtcaac aagcaatcac
     2521 ctttgccgcg gaaggcccct cgtttggcaa cgacctcaac acccggctca aagatgatct
     2581 ccttgttacc gataagctcg agtttatcga ccacccaagg gctggccatc ttgggaataa
     2641 tcactctttt tgccttgcat cgcagggctg cctgaagaca ttgggtagaa tcttcgggat
     2701 agaagcccca ccaggctgca ttagccgtat cgcgctggcc cgcctcaact tcttgtattg
     2761 cctgttcatt ggggctcacc gcccctgttg actccgcagc caaacaacga tgaaaaccca
     2821 gtacaaggca accggcggtg agaaccatca cccaaggcca catcctcata ttcatgtcga
     2881 tctcctccgg cagaaaacgc ccgatcaacg acgtaggttt cgaccacaaa gggtacaaaa
     2941 gcccgtgttt cctgtgcaaa gctgcactcg cttcacgggc ccctcggtca gacgctgagg
     3001 ccttacgtat tctaactttc tccaaaatgc tcggccacaa cgtcgttatc tgggcaacgt
     3061 gccaaatgaa agccggcacc accgtcggcc gaagcagcgc gttggattcg gcaaaacttt
     3121 atccaaaatc acctacagcg accagccagt tcgatacggg ggacggagta aggcgtccgc
     3181 ctcggcattg ccgataatgc gacattccac ggggtcaaac tcaatcggtt cgtcaaagcg
     3241 accggccacg ttcgccacca gcaacaattc gatgactggc cccgagtgat cgaactggga
     3301 caagcagctc ccttgtcctc ggcaggcatt aagccactcg tattcgtggc ccgccacatg
     3361 gggataacgc ttcggtctgc ccgcctgctg ggggaatttg tccgccggca ggatttgaca
     3421 gacggaatta tgagcattgg tatgcacgac gcccttttct cccacgatca aggtccccga
     3481 acgtggcgtc cagctattgt ccttccattc gagatcgcgg cccgcaatat cttccaattt
     3541 tttccagact cctcgacgat gtaactcagc ttcgtcggcg ttgtaccaat agattgtcgc
     3601 cgggggttgg tcaccccttg ccgggatgta aaaccggaca aattgccacc taggaaaacc
     3661 gtgtggtgcc ttttccggaa tttcgctttc cacgcgaatt cgcttttcgg aattatgggg
     3721 cggattccaa agttgacgta ggcccaaggc tttgaaggtc agatgaagcg aatgcgaacc
     3781 cccaccgccc aaacagcccg tgccaaagtc ccaccacctt ccccagcctg aaacgtactg
     3841 gggatggtat ggcctttcgg cagccggtcc aagccaggca tcccagtcca agcccgaggg
     3901 aacgggcggc gtgtcctggg gaggttgcct cgggccgct
//
LOCUS       DAOPTR010000259         3938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_18394_length_3938_cov_3.117177, whole genome shotgun sequence.
ACCESSION   DAOPTR010000259 DAOPTR010000000
VERSION     DAOPTR010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18394_length_3938_cov_3.117177"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="PKI05_06165"
     CDS             complement(<1..1141)
                     /locus_tag="PKI05_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOA51822.1"
                     /translation="MAPLPGFLAALRTAIVAAIFVFVVVVLLVWNALHQVNKDSLDSP
                     QYAELQKKLSLDPQNEELKQQIRELDLQLRQAYFRSRTFNRWGTMLLIAGLIVFAVAA
                     RIASGLRRPLPRPTATEIWDESFDRATRLRRFAILGMAGLLVAVACVFWAVGRSVIKD
                     TLAVREAAEVPPREVAAVVTSPTESTVVQNGSRGMGSSQQGKAIFTEEYRRYWPRFRG
                     PDGSGISPHPNPPVSWNGKTGEGVLWKVAVDLPGNNSPVVWANRVFLTGATEERRCVY
                     CFDADSGKLLWQQDIPATPESENKKPKVMKDTGFAAPTVATDGQRVYAIFANGDLAAL
                     DFEGKLLWTKGFGVPDNAYGHASSLCTYGDLVIVQLDQGTPDDDLS"
     gene            complement(1301..2650)
                     /locus_tag="PKI05_06170"
     CDS             complement(1301..2650)
                     /locus_tag="PKI05_06170"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOA51823.1"
                     /translation="MSRFSIGLLAIILALVWTYASRAVERFPPPDFTSHQLPETPQPP
                     PPAPWWETIDVVLLVTALVLATYFALAKRSRNATLVLTGFSLFWFGFHRQGCVCPIGA
                     IQDVAQGLFDSAHAIPWSILVFFSLPLVFALFFGRVFCAAVCPLGAIQEIAAIRPHKL
                     PDWLEQMLGLFPWVYLGLAVLYAATGTAYVICEYDPFVAFFRRSGATSMLVFGGALLA
                     IGIFVGRPYCRFLCPYGVILGLFAKLARWHVRIPPTECIRCRLCEDACPYNAIVAPVP
                     PLTRSERAPARRRLLLAMAVLPIWLALGGGIGYALHPIMAQLDPQVRLARWVRSKNID
                     PNNKFAVDAVTAFHNTGASEASLYQAAAERLRKFGVGSAIFGLWVGLVFGLKWVHTST
                     RPSRQEYLPDPRRCVACGRCFWYCPVEQVRRGWIIEDELLQLPKERAPLVSSVANDE"
     assembly_gap    2753..2762
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2812..3399)
                     /locus_tag="PKI05_06175"
     CDS             complement(2812..3399)
                     /locus_tag="PKI05_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOA51824.1"
                     /translation="MNNSQPAQRIPRRELLREGVQMAAFLGVGGLVGFVAGRKATSEP
                     LVWQIDPYKCIACGNCATHCVLDPSAVKCVNSFPICGYCDLCTGYFPPEPAALNTGAE
                     NQLCPTGAIIRKLVEEPYYEYTIDEELCIACGKCVIGCAAFGNGSLYLQVRHDRCLNC
                     HECSIAIACPSQAFVRVPASEPYLLKHKYKPAAPA"
BASE COUNT          971 a         1187 c         1000 g          770 t
ORIGIN      
        1 ttgaaagatc atcgtcgggg gtcccttgat ccaattggac gatgaccagg tcaccgtagg
       61 tgcacaaaga actggcatgg ccataggcgt tatcgggaac tccaaatccc ttagtccaca
      121 gcaacttgcc ctcaaaatcc agggcggcca agtcgccgtt ggcaaaaatc gcatacactc
      181 gttgaccatc cgtcgccacc gtgggggcgg cgaagcccgt gtctttcatg accttgggtt
      241 ttttgttctc actttccggc gttgccggaa tatcctgctg ccaaagcagc tttccggaat
      301 cggcatcgaa gcaataaacg cagcgtctct cctccgtggc ccccgttaaa aacacacgat
      361 tggcccaaac cactggggaa ttgttgccgg ggagatccac tgccactttc cagaggacac
      421 cctcgccggt cttgccgttc cagctcacag gtggattggg atgaggcgaa atgcctgacc
      481 catctggccc ccggaatcgt ggccagtaac gtcgatactc ttcggtaaaa atggccttcc
      541 cctgttgcga gctccccata cctcgggaac cattctggac aacagttgac tcggtcgggg
      601 aggtgaccac ggcggccacc tcgcgtgggg gaacctctgc ggcttcccgc actgccagcg
      661 tatcctttat caccgatcgc cccacggccc aaaagacaca tgcgacagcg actaggagac
      721 cagccatccc caaaatggca aaccgccgga gccgcgtggc gcgatcgaag gattcatccc
      781 atatctccgt tgccgtggga cgtggcaggg gacggcgtaa tccagaagct attcgggccg
      841 ctaccgcaaa gacaatcaga cccgcgatga gaagcatggt tccccagcga ttgaacgtcc
      901 gactgcggaa ataggcttga cgaagctgca aatcgagctc tcggatttgc tgtttcagct
      961 cctcgttttg ggggtccagg ctcaactttt tctgcaattc cgcatattgt ggtgaatcga
     1021 gagaatcttt gttgacctga tgcagagcgt tccagacaag gagcacgact acaacaaaca
     1081 cgaagatggc tgccacaatt gccgtccgga gtgctgccaa aaaacccggc agaggagcca
     1141 ccaacgctcc tggagtctcc accctcaagt ttgcgggggc caactgagct tcacccttat
     1201 ctggtacatt tgactcttca cgcctttgac tcatcatgac cacccggtat ccaatgcctc
     1261 acgtgatgaa tttccaaaca aaaacggatg caccaccctc tcattcgtca tttgccacgg
     1321 atgaaaccaa tggcgctcgc tctttaggca gttgtaacaa ttcgtcctca atgatccatc
     1381 cgcgcctgac ttgctccacg gggcagtacc aaaaacaccg gccgcaggca acgcagcggc
     1441 gcgggtcggg aagatactct tggcggctgg gacgcgtaga ggtgtgaacc catttcagac
     1501 caaacaccaa gccaacccag agcccaaaaa tcgcgctccc cacgccaaat tttcgcagtc
     1561 gctccgcagc ggcttgatac agtgatgctt cacttgcgcc cgtgttatga aaggccgtca
     1621 ccgcatccac ggcaaacttg ttgttgggat caatgttttt agaccgtacc caccgggcca
     1681 agcggacctg aggatcaagt tgggccatta tcggatgaag agcgtatccg atcccaccgc
     1741 ccagtgccaa ccagatgggc aagaccgcca tggccaaaag caagcggcgt cgggccgggg
     1801 cacgttccga tcgggtcaaa gggggaacgg gcgccacaat ggcgttatag gggcatgcat
     1861 cttcacacaa acggcagcga atacactccg tcggaggaat ccgcacgtgc caccgggcca
     1921 atttggcgaa aaggccaagg atcactccgt acggacaaag aaaacggcag tatggccgcc
     1981 ctacgaagat cccgatcgcc aatagcgcac ccccaaaaac aagcatcgag gtggcaccac
     2041 tgcggcgaaa gaaggccaca aatggatcgt attcgcagat cacataggcc gtcccggtcg
     2101 ccgcatagag cacggccaat cccaggtaaa cccaagggaa caaccccaac atctgctcta
     2161 gccaatcggg gagcttgtgg ggacggatcg cggcaatctc ttgaatcgct cccagcgggc
     2221 agacggcagc gcaaaatacc cgtccaaaaa acagggcaaa tacaagaggc aaactaaaga
     2281 atactaagat gctccaggga atggcgtgtg cagaatcaaa caacccctgg gccacatctt
     2341 ggatcgcccc aattggacag acacatccct gccgatgaaa gccaaaccaa aacaatgaaa
     2401 aacctgtcaa aacgagtgtg gcattccgtg atcgcttggc caaagcaaaa taagtcgcca
     2461 aaaccaaagc cgtgacaaga agaacgacat caatcgtctc ccaccacgga gcgggcggtg
     2521 gcggttgcgg tgtctccggc aactgatgag acgtaaagtc agggggcgga aatcgttcca
     2581 ccgcacggct ggcgtatgtc caaacaagag caagtatgat ggccaacagc ccaatcgaaa
     2641 agcgactcat tctccgcctt ggccgcgggg ctttggcaca acacggctcc aggtgcttat
     2701 gcaattcttg atcactcaca gtcgtcatga atttcagtct ttcgccttaa ccnnnnnnnn
     2761 nntaacctat atcgcctgac ctgcctaccc gaaggaacac agttcgacac atcaggcagg
     2821 agcggcgggc ttgtacttat gtttcagaag atatggctca gaggcgggca ctcggacaaa
     2881 ggcttgcgac ggacaggcaa tcgcaatcga gcattcgtgg caattgaggc atcgatcatg
     2941 ccggacttga agatacagtg acccattgcc aaatgccgca caaccgatca cacatttccc
     3001 acaggcgatg caaagctctt catcgatggt gtactcatag tagggttcct ccaccaattt
     3061 acggataata gcgcccgttg ggcacagttg gttttcggca cccgtattca gtgccgccgg
     3121 ctcaggggga aagtaaccag tacacaaatc gcagtagccg cagatgggaa aactgttcac
     3181 gcacttcacc gccgacggat ccagcacaca atgtgtggcg caattgccac aggcgatgca
     3241 tttatagggg tcaatttgcc acaccagggg ttcggacgtg gccttcctac ccgccacaaa
     3301 accgaccaag ccacccacac ccaagaaagc ggccatctga acaccttccc gcaaaagttc
     3361 ccgtcgaggg atgcgttgtg ccggttgact gttattcacg ggttgtggct ccttctttct
     3421 cagaaatcac cgaagtgagt cagggtgacc gtcctccatc gtgagcctca cttgtcatgc
     3481 ccactggaaa tgcctgtctc ccctacttgt tttgtgggcc gaagggcaca gcctgccccc
     3541 caaggacaat gatcgcagcg tgccagatca cccgacgggc aacgacccgt tcgttgccct
     3601 aagaaaaaca ccatcccagc taggcccccc aaaaccaaag cccgaagtcc cgcacggact
     3661 agttcacgtc gggaaattcg aagtgttacc acctctctcg tcatcgctgt aaccattctt
     3721 ttgtttttgg ggcatcgagg gtctaaaccg ttccgttcac acctcaccta actcaatgcg
     3781 cctcatccgg cgttccagcg gacgccgctc cgctactcgg cgtcttgtcg tttggctctt
     3841 ccggccgctt gggtcgaaat gcctcgagga cctttctcgc gggatcaaga atcacgatcc
     3901 attcttcgcc ttcccctcga tcctcgacta cctgaaag
//
LOCUS       DAOPTR010000260         3928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_18449_length_3928_cov_3.178415, whole genome shotgun sequence.
ACCESSION   DAOPTR010000260 DAOPTR010000000
VERSION     DAOPTR010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18449_length_3928_cov_3.178415"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PKI05_06180"
     CDS             <1..580
                     /locus_tag="PKI05_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51825.1"
                     /translation="GPVPQSLAEVMKLTDSGPAYSEVNTADAMTLTDDDTPTRRHLQL
                     RMGCRTEILASAVYEELPFLRREDGRLVGRTLPKAQGVFALTTRPGTYGGTRIRLVPE
                     VHFGEPRIQPIAKQGMIRWESQRERVVLDDLIVETELQPGDLLVLTCLPNCPGSLGNR
                     FFTVTSQGKMHQKIIIIRLSQTQASSLFTANE"
     gene            complement(735..1718)
                     /locus_tag="PKI05_06185"
     CDS             complement(735..1718)
                     /locus_tag="PKI05_06185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HOA51826.1"
                     /translation="MIVTTKELFKHAYGKYAIGAYNINNLEQTVGLFRGNLGKIKKNE
                     DPVDYSVAAPFILQISRGARSYTDKRFLEAMIRAAETVFPEAIFAVHLDHGTEEVCYD
                     AIDSGFYSSVMIDASHEPFQKNVEITKRVVDYAHKKGVVVEAELGQLGGVEEDIEGSV
                     HLTDPEEAAKFVELTGCDSLAVAIGTSHGAYKFPGAQGVRLDRLAQIQKALPEGYPLV
                     MHGASSVPKEWVDRINAAGGALKQTSGVNEDDYAKAAALGVCKINIDTDGRLVWCAVY
                     RETFRDKPEDFDPRTPGKVFMAAYAEYIIHKNKKLGSAGQLKEVAAFVRGG"
     gene            2155..>3928
                     /locus_tag="PKI05_06190"
     CDS             2155..>3928
                     /locus_tag="PKI05_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51827.1"
                     /translation="MSRTSETDHFLTNGPALGSVVFLSWMLIIDVSLTVASEEPPAVI
                     QQSTASVTLIPNPDFREGDDSPSAWKLSNGAGHWLDRDILEIEGRGQGSSYWYTNVSL
                     QPRSVYLFRMVARQIGGGSGCVITGPATVNHDYRLTEAWREYSHIFRMPDEPISKSLF
                     RLGQWEMSGRVQFDKVELLPVLPIHARNNECVLGQGESIRDGIYSFEGTFDGIGSNDH
                     RVLHRATARFNTNRWVFSEDEEVVYKFQLPETKFLSGQLSFAVNYATGGTMLAEVSPD
                     GTSWTEAARQDGVGVQQTELPASLFPASAIFVRLRPVGTAASMQVDRIEFRGKIDRHL
                     SDFQGITTYGVWRPLADSISHPAIDLHITGASGSMSRSLAVHPRDVPEPKLDASVIDS
                     EKKTHPIAVVPATSSAQNSGGWTITLPQLGPGQHLCRINLSSQGQTLGVLEIPWEVNQ
                     YYRADYGELLSSQPDAVIWWCEADWKIPRDRPPPSGFPSPSSMVRLTSARNDYESVQV
                     VISPQADGRLKSVEVGLLRHPSGATIAGDDIKVSYVYYHYVHTPTDKEGVRDWWPDAL
                     PPCDSPLSLVKGQNQPLWITVHVTE"
BASE COUNT          857 a         1155 c         1014 g          902 t
ORIGIN      
        1 aggaccggtg cctcaatcgc tggccgaagt aatgaagctc actgacagcg gaccagccta
       61 cagcgaggta aacacggccg atgccatgac gctaacggac gacgataccc ccacccggcg
      121 tcatctccag ttgcgtatgg ggtgccgcac cgagattctc gcctccgccg tttacgagga
      181 actccctttt ctccgccgcg aggatggccg gctcgtagga cgcacccttc caaaggccca
      241 aggcgtgttc gccctgacca cgcgcccggg aacatacgga ggcacccgaa tccgacttgt
      301 ccccgaagtc cattttggcg agcccaggat tcagcctatc gccaagcagg gcatgatccg
      361 atgggaatcg caacgggagc gcgttgtact ggatgatctt atcgtcgaaa cggaactcca
      421 acccggagac ttgcttgttt taacgtgcct tcccaactgc ccaggcagtc tgggaaaccg
      481 attctttacg gtgacctccc aagggaagat gcatcaaaag attattatta tccgcctgtc
      541 ccaaacacag gcttcgtcac tctttacagc gaacgaatag gattcgtctt ccccgtcaag
      601 aacgcaactc gggtagaacg agactagcta tcttacgaac cggcgacaaa aaggccgcgc
      661 tccggacgct gccgaatcgc ggcacgttct ttcttagcac cgtttaccgg acaacgagtc
      721 agcgagtttt tgcttcaacc accgcgaacg aaagcggcaa cttccttgag ctggcccgcc
      781 gaccctagtt tcttattctt gtggatgatg tactccgcat aggcggccat gaagaccttg
      841 cccggtgtcc tcgggtcaaa atcttcaggc ttatcgcgga atgtctcgcg atagacggca
      901 caccatacga gcctgccatc agtatcgatg ttaattttgc acacgcccag cgcggccgcc
      961 ttcgcgtagt cgtcttcatt cacgccgctc gtctgcttta gtgctccgcc ggcggcattg
     1021 atgcgatcca cccattcctt tggaacgcta ctggcaccgt gcatgacaag gggataaccc
     1081 tccgggaggg ccttttgaat ctgggccaac cgatccagcc gcaccccctg agcgcccgga
     1141 aacttgtagg ccccgtgcga ggtaccaatg gccaccgcca gcgaatcgca tcccgtcagc
     1201 tccacaaact tcgcggcttc ctctggatcg gtgaggtgaa cactgccctc gatatcctcc
     1261 tcgacacctc ccaattgacc gagttcggcc tccaccacaa cccccttctt gtgtgcgtag
     1321 tcaacaacac gtttcgtgat ttccacattc ttctggaaag gctcgtgaga ggcgtcgatc
     1381 atgaccgaac tgtaaaatcc gctgtcgatc gcatcatagc acacctcttc ggtaccgtgg
     1441 tcaagatgca cagcgaagat ggcctcgggg aaaacagtct ccgccgcacg gatcatagct
     1501 tccaggaatc gcttgtcagt ataggaccgt gccccgcgag aaatctggag gataaagggg
     1561 gcagccacac tgtagtcaac agggtcctca ttctttttga tcttccccaa atttccgcgg
     1621 aatagaccca cggtctgctc taggttattg atgttatacg ccccgatggc gtactttcca
     1681 taagcgtgct tgaaaagttc tttcgtggta acgatcatga gacgtctcct cgtgctagag
     1741 ccaaaaaacc ggcaatcaca ctctgttcaa ctctcttgaa taaccacgcg cgcggaccga
     1801 tggaacatcg aacggcaaac cggtcacaca gtagaatctc aagctgcctc ggatcaatgg
     1861 cgtgcaacaa ctgtggctaa ccatgagagt tgatacctac tcgtaattta tcgttttttt
     1921 gcgatgttga ccagactttc gcatcagaat tgtggcacag tcgataggaa tgtggcacaa
     1981 ctgtggcgca aactagctcg attgtttctc gtccacgccg gcgatacttt ggcaaacttg
     2041 caaacgccca tcgcagtgtg gacgttgcga tgttgatgtt agtttatcat aatgctggca
     2101 tttcggccga tcgcaatccg accaattgtc gcctgactct ttggaggaac tgacatgagc
     2161 aggacgtctg agaccgacca tttccttacg aatgggcccg ccctcggtag tgtcgttttt
     2221 ctttcctgga tgttgattat tgatgtttct ctgaccgtgg cctccgagga accccccgcg
     2281 gtcattcaac agtcaaccgc aagtgtcacg ctaattccaa accccgattt ccgagaggga
     2341 gacgactcgc catcagcgtg gaagttgtct aacggtgccg gtcactggtt agatcgggat
     2401 atcctggaaa tcgaaggccg cggtcagggt tcctcttact ggtacacaaa tgtgtcgctg
     2461 cagcctcgct cggtctatct ttttcgaatg gtcgcccgcc aaatcggtgg tgggtccggc
     2521 tgcgtgataa cgggccccgc tacggttaac catgactacc ggttgaccga ggcctggcgc
     2581 gagtacagcc atattttccg catgccggac gaaccaattt caaagagtct tttcaggctc
     2641 ggtcagtggg agatgtcagg acgtgttcag tttgacaaag tcgaacttct gcccgttctg
     2701 cctattcatg cccgcaataa cgaatgtgtc ttaggccagg gagaaagcat tcgcgacgga
     2761 atctactcgt ttgaaggaac atttgatggt attggcagca acgaccatcg ggtccttcat
     2821 cgggccacgg ccaggttcaa cacaaatcgc tgggtctttt cggaagatga ggaagtggtt
     2881 tacaaattcc agctaccaga aacaaagttc ctgtcgggcc agctcagttt cgctgtaaat
     2941 tacgcgacgg gtggcaccat gttggccgag gtcagccccg acgggacatc atggacggaa
     3001 gccgcacgac aagacggcgt cggtgtccag cagacagaac ttccggcctc tctcttcccg
     3061 gcatcggcca tctttgtcag gcttcgtccc gttggcacgg ctgcctcgat gcaagtcgat
     3121 cgtatcgagt tccgcggtaa gattgaccgt cacctctccg acttccaggg catcaccacc
     3181 tacggggttt ggcgtcccct cgccgacagc atttctcatc cggccattga tctccatatt
     3241 accggtgcga gcggctcgat gtcccggtct cttgctgtgc acccacgaga tgtgcccgag
     3301 cccaagttgg atgcctcagt catcgattct gagaagaaga cgcatcctat cgctgtcgtc
     3361 ccggcaacat cttcggccca gaactcaggc ggctggacga ttacattgcc gcaacttggt
     3421 ccgggccaac atctttgccg tatcaatctt tcgagccagg gccaaacact tggtgtcctc
     3481 gagattccat gggaagtcaa tcagtactac cgcgccgact acggggaact actcagcagc
     3541 caacccgacg cggtaatttg gtggtgtgaa gccgactgga agattccccg cgatcggccg
     3601 ccgccaagtg gtttcccgag cccgagttcg atggttcgcc ttacctcggc tcgaaacgat
     3661 tatgaatccg tccaggtggt gatttcgcct caagctgacg gacgactgaa atctgtggaa
     3721 gttgggctct tgaggcatcc gtctggcgcc acaattgccg gtgatgatat caaggtctct
     3781 tacgtgtatt accattacgt tcacacgccg accgacaagg aaggcgttcg cgactggtgg
     3841 cccgacgcct tacctccctg tgactccccg ctttctctgg tcaagggtca gaatcaacca
     3901 ctgtggatta ccgttcatgt gaccgagg
//
LOCUS       DAOPTR010000261         3925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_18468_length_3925_cov_3.487339, whole genome shotgun sequence.
ACCESSION   DAOPTR010000261 DAOPTR010000000
VERSION     DAOPTR010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18468_length_3925_cov_3.487339"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1403)
                     /locus_tag="PKI05_06195"
     CDS             complement(<1..1403)
                     /locus_tag="PKI05_06195"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HOA51828.1"
                     /translation="MKTRRTLTGILVLGLMALGSQLRAADLEIELIPELDLFGNQIEV
                     IQGYQWGGEMALAFGIYDTGASVVTLSAADRAFFELQGSVVPIKVPGGAQADAIDGVV
                     IGDVSMPMTFWADGLHAVSINWSSVSWEMEFTYDLSGGATVSGVQVFVGTEEGSPQLP
                     NIAGTPIHLGTLTGSTERPAAKIDMLGYEFDFGEFFSDDPFLGPLFQGIVFYMPDLAF
                     VPSAMRLAGIAGETTDPVRIPLSLFGPTNHPDPGDETTVAPNPVQAQVSLQEALMTVA
                     DALFLFDTGASVSVITTALAEQLGLNLNSPETTLDIVGAAGAPITVPGFTLDSLSIPI
                     DSDFDGLTDGVLRFLNAPVFVLDLVEGLDGILGMNLWNTSKEMLYDPFDPLGPSLQVT
                     FLTAAREIPSDEEWELLQLLAALDPMFGQLLGIPSGPLSFPRFEMRGAGGDLGGGGEE
                     VIPEPGSLTILVLGGGLL"
     gene            1856..3709
                     /locus_tag="PKI05_06200"
     CDS             1856..3709
                     /locus_tag="PKI05_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOA51829.1"
                     /translation="MSNNPQSLLPPDDQQGSSSKTSQPASETNQGRDSQETEARPNRP
                     RILIGSQRDPAAYRPRPGRPLTPFRSKPTVPGQAGGKEDTGSPGDGLAGGSPQSPLPA
                     VTTQSEVKTETQRTSQEQETESASLPEPKMETAQPAEALSPSSPPLVSSDARLFDLPE
                     EDEKERELDSELAKLQAEARDVALVLKKEPADQSLFNRRLGLPPDLEKEYAEALGDVP
                     VEELIQETSAKFKVGQLEPESRHVGKVLAVRGENVFVELGLREQGIVPLRSFREPPEV
                     GQSVEVRVVRFVPNEGIYELVLPAAAADVADWADLSEGMIVEARVTGVNQGGLECEVN
                     RIRGFIPISQIDIFRVEKPEEYVGQVFTCVVLEADAQRKNLVLSRRAILEREREQAQR
                     LLWDSLAPGQVREGVVRKLMDFGAFIDLGGVDGLLHVSQLAWGRVKHPSEILREGQTV
                     RVRIDKVDRENRRISLGYRDLISNPWEDAEKKYVPNSVWRGTVTRLMDFGAFVELEPG
                     VEGLVHISELALKRVSRVSDVVKEGDVVDVMVLSFDPQERRISLSIRAALKLKEAQTQ
                     EQAETAEAGTPAEAAAPEPPPAPPKKRKPTGPLKGGLGSAPSGGGIFGLKW"
     gene            3713..>3925
                     /locus_tag="PKI05_06205"
     CDS             3713..>3925
                     /locus_tag="PKI05_06205"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665013.1"
                     /note="catalyzes the radical-mediated insertion of two
                     sulfur atoms into an acyl carrier protein (ACP) bound to
                     an octanoyl group to produce a lipoyl group; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOA51830.1"
                     /translation="MDAEHSRDAVPIIEGRVGITAAPACATTVASSGLPRWLKRRIPK
                     GNGNHFTARLIESLGLATVCEHARCPN"
BASE COUNT          851 a         1082 c         1206 g          786 t
ORIGIN      
        1 agcaggccgc cgccaagcac gaggatggtc aaactgccag gttcggggat gacttcttcg
       61 ccaccgcctc ccagatcgcc gcctgcaccc cgcatctcaa accgcgggaa ggacaacgga
      121 ccactcggaa tccccaacaa ttgtccaaac atggggtcca aagcggcaag cagttgtagc
      181 aactcccact cctcatcgga cgggatctcc cgcgctgccg tgaggaatgt cacctggagc
      241 gaaggcccca ggggatcgaa ggggtcgtag agcatctcct tggaggtgtt ccaaagattc
      301 atgccgagga tgccgtccag gccttccacg agatcgagga caaaaacggg tgcgttgaga
      361 aaccgcagga ccccatcggt aagcccatca aaatcactgt caatcgggat tgataagcta
      421 tccagcgtga aacccggcac ggtaatcggc gccccggcag caccaacgat gtccagtgtc
      481 gtttcaggcg aattcaagtt tagcccgagt tgttcggcca aagccgttgt gataacactc
      541 accgacgccc ctgtatcaaa gaggaacaag gcatcggcta ctgtcatgag ggcctcttgg
      601 agggagacct gggcttgcac gggattgggt gccaccgtcg tttcgtcgcc cggatcaggg
      661 tgatttgttg gtccaaacag cgagagcgga atccgtaccg ggtcggtcgt ttcgcccgct
      721 atcccagcca gtcgcattgc cgaagggaca aatgccaggt ccggcatata gaaaacgatg
      781 ccctggaaga gtggtcccaa gaaaggatcg tcagaaaaga attcgccgaa gtcgaactca
      841 taacccaaca tgtcaatctt ggctgcggga cgctccgttg agccagttag ggtgcccagg
      901 tgaatgggtg tccccgcaat gttggggagt tgcggacttc cttcttcggt tccgacaaac
      961 acctgaacgc ccgaaacggt agccccaccc gataagtcat aagtgaattc catttcccaa
     1021 gagactgacg accagttaat ggagacggcg tgcagtccgt cggcccaaaa ggtcatcggc
     1081 atcgaaacgt cgccaatgac aacaccgtcg atggcgtcgg cttgtgcacc tcctgggact
     1141 ttaatgggca caacggaccc ttgcaactcg aagaaagccc gatccgcagc cgagagagtt
     1201 accacgcttg cgcccgtgtc atagatgcca aaggcgagcg ccatttcccc gccccactga
     1261 taaccttgga tgacctcaat ttgattcccg aagaggtcca actcaggaat aagttcaatt
     1321 tcgaggtcgg ccgcgcggag ttgcgagccc agggccatta agcccaggac aaggataccc
     1381 gtgagcgttc gccgcgtctt catggcaaag ttcccccgat gcgatcgcca accattcccc
     1441 cgaggagttt ccctggcgcc tgtaacagcc ctcccaaatt ccgcccggca ttataatctc
     1501 cccaaattca attgcaagca ctcgaaatga ttttctacaa gcgtaggagt gtcatatccc
     1561 ctcttctggg ggtggcctta tcctggcaga atctcttcga gaaggcttca aggggtgttt
     1621 cctcttgtaa gaggcggctc cccctttttg ccggggcagc tttgccggtg ggcgaattgg
     1681 cgatgtcgca cggcgcttgg gcctgccgca tcgctgcctg gagcaaatac ccgcagggcg
     1741 gtagaataaa gtgtttggca ttggccccgg accgtggcct ttggcagtaa agggccgcgg
     1801 gccatggtgg ctttgcgatt tggtgaccac cagttgtgat gtgagttttg gtccgatgag
     1861 taacaatccg cagtccttgc ttcctcctga tgaccagcag ggttcctcca gtaagacttc
     1921 tcagccggcg agtgagacga accaaggaag ggattcccag gaaacagaag cccgccccaa
     1981 tcgtccgcga atcctgattg gttcacaacg agacccggca gcctatcggc ctcgcccagg
     2041 gcggccgctg acgccgtttc gaagcaaacc cacggttccc gggcaggcag gaggtaagga
     2101 ggatacaggc tcccctggcg atggtctcgc cggtggttcc ccacagtctc ccctcccggc
     2161 ggtgacgacc caatcagagg tcaagacgga gacccagaga acatcgcagg agcaggagac
     2221 cgagtcggcg tctttgccgg agccgaaaat ggagacggcg caacccgcgg aggcattgtc
     2281 gccgagtagt ccgccgcttg tctcttccga tgccaggtta ttcgatcttc ccgaggagga
     2341 tgagaaggaa cgcgagttgg attcggaatt ggccaagttg caggccgagg cccgggatgt
     2401 ggcgcttgtc ctaaagaagg agccagccga tcagagcctt tttaatcgtc ggctaggatt
     2461 gccgccggat ttggagaagg agtatgccga ggccctgggt gatgtaccgg tggaggagtt
     2521 gatccaggag acatcggcga aattcaaagt tgggcagtta gagcctgagt cgcggcacgt
     2581 gggcaaggtc ttggcggtcc gtggtgagaa cgtctttgtg gagttggggc tacgggaaca
     2641 gggaatcgtt ccactgcggt catttcgtga accgccggag gtcggtcaat cggtggaggt
     2701 ccgcgtggta cgatttgtcc ccaatgaggg gatttacgag cttgtcctgc ccgcggcagc
     2761 ggccgatgtt gccgattggg ccgatctgtc ggaaggaatg atcgtcgagg cccgcgtgac
     2821 cggtgtcaat caagggggct tggagtgcga agtcaaccgt atccggggat tcattcctat
     2881 cagccaaatt gatatcttcc gggtggaaaa gcccgaagaa tacgtgggcc aagtcttcac
     2941 ctgtgtcgtt ctggaggccg atgcccagag gaaaaatctg gtgctgagtc gccgggctat
     3001 tttggagcgt gaacgggaac aggctcaacg gttgctatgg gattctctcg caccaggcca
     3061 ggtccgcgag ggcgttgtgc ggaagctgat ggactttggg gccttcattg acttaggcgg
     3121 cgttgatggt ttgctccacg tcagtcaatt ggcgtgggga cgtgtcaagc atccaagcga
     3181 gattctccgc gaagggcaaa ccgtgagggt ccgtatcgac aaggtcgatc gtgaaaatcg
     3241 ccgcatcagt ttgggttacc gtgatttgat ttcgaacccc tgggaagacg ccgagaaaaa
     3301 gtatgtgccg aattctgttt ggcggggaac ggtcacgcgg ctgatggatt ttggggcctt
     3361 cgtcgagctt gagccgggcg tggagggcct ggtgcatatc tcggaactgg cactcaagcg
     3421 ggtgagccgt gtctcggacg tggtcaaaga gggcgatgtc gtggatgtga tggtgctttc
     3481 ctttgatccg caggaacgac ggattagcct ttctatccgt gcggcgctga aactgaaaga
     3541 ggcccagacg caagagcagg ccgagactgc tgaagcgggg accccggcgg aggcggcagc
     3601 tccggaacct ccgcccgctc cccccaagaa gcgcaaaccc accgggccgc tcaagggagg
     3661 cctgggaagt gcaccttccg gaggaggtat ctttggtttg aagtggtgat ggatggatgc
     3721 cgaacattcg cgtgacgctg ttcccataat cgaaggacga gtcgggatca cggccgcacc
     3781 ggcctgtgcc acgacggttg catcgagcgg tcttccgcga tggctcaaac gccgtattcc
     3841 aaaaggaaac ggcaatcatt tcactgcccg gctgattgag tcgctcggcc tggctaccgt
     3901 ctgcgaacac gcccgatgcc ccaat
//
LOCUS       DAOPTR010000262         3914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_18542_length_3914_cov_4.017880, whole genome shotgun sequence.
ACCESSION   DAOPTR010000262 DAOPTR010000000
VERSION     DAOPTR010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18542_length_3914_cov_4.017880"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(260..616)
                     /locus_tag="PKI05_06210"
     CDS             complement(260..616)
                     /locus_tag="PKI05_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51831.1"
                     /translation="MSLDQLDEVSKKIATLIYQSVPIDEWVEANLIWKMSPNKKNSAS
                     RTQYLLPDGTVDKSKGLDVTTNMKLYDLLVKHRDLSEEMGQPWWFQITVTVNRSGTFK
                     MDFEYRENYRPEDLAL"
     gene            complement(629..1117)
                     /locus_tag="PKI05_06215"
     CDS             complement(629..1117)
                     /locus_tag="PKI05_06215"
                     /inference="COORDINATES: protein motif:HMM:NF025298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HOA51832.1"
                     /translation="MASRRPSVVRATAGRRGNWTAELRHPRPNAMYVLDNGHVYRTDA
                     LGRVESVEGRLSLDRLDRNTSAQIRAGREGGTGYDGGHLIGHGVGGAGDGINLVAQMS
                     QVNRGEYRVLEGQWAEAMRAGQTVDVRVVPVWSDASSTVPSVLQVEYWIDGVRGSQLF
                     VN"
     gene            complement(1189..1581)
                     /locus_tag="PKI05_06220"
     CDS             complement(1189..1581)
                     /locus_tag="PKI05_06220"
                     /inference="COORDINATES: protein motif:HMM:NF016515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HOA51833.1"
                     /translation="MSLYKLDQLINKIASLVHEASPIPDWTRVCYVAKLPPDLQTSIN
                     QFFFKREDASVVADATKVDIQLRSKLTQLLIEHRKVTEELGQPWWYQIEVCVDRDGSF
                     RTRFEYTETFDERRFMFDRIDVPKAMEE"
     gene            complement(1600..3552)
                     /locus_tag="PKI05_06225"
     CDS             complement(1600..3552)
                     /locus_tag="PKI05_06225"
                     /inference="COORDINATES: protein motif:HMM:NF025298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HOA51834.1"
                     /translation="MPAQVWYGEVNAGEHYDALEASAATLQNEQHTSDDRASAYYPPH
                     QVVVVGGDRVSVFGESEQAAEAPVGATGAPAKRLASFADVQSGAELGRGGTGSDSLGP
                     DESATDRDMVLTTNTLRWVEMPWGGEWELIDDDSYQSPWVLVEVPVDWDQGQEAPIPV
                     EPPIEPPMAPPTELVPVWPSGPVDPETGQPTLQPVEVIEGRIQWPPGTTFVRVPWEPP
                     AYGSPGSGGSGSENTPDVLAISRNEDGGYSVRNAAGHMVATVTPQAAVQVEAQGLSVQ
                     QFVEAAFNHPGVRTALTENQSAPVSLGIRVSEDGSTYNVELTVGGAPGSGVSYPVLDS
                     DLNPVQGPPQYGSANPSPGNGDYPLTEELRAELDSLVNSPPDPEWPAERSALREWLDE
                     RFQEIESAIQAILSPEERVRTAETVLGLVPGIGLTFSIYEFIFGTSPLSGDDASRAIA
                     LLGIISGGYGDDALRLVRGGLRSSLADHIADLTNIARSEGRDLVRATPGSRGNWTADL
                     RSPRPNAMYVLDNGHVYRTDALGRVESVEGRLSLDRLDRNTSAQIRAGREGGTGYDGG
                     HLIGHGVGGAGDGINLVAQMSEVNRGRYRVMEREWAQHIADGHTVDVRVTPIWNDSNP
                     SVPSNMVVESWINGVPQPPIEYPNIR"
BASE COUNT          823 a         1248 c         1004 g          839 t
ORIGIN      
        1 tggaacacgc gaagttgaga tcaaaaccag tgcggtcagg ctactgagac cggccaggat
       61 gttcatgagt gcggccaacc aaagacgctt tggtcgcttg aattgtggtg aagtcacata
      121 cgctccctaa tgagcccaga cggcaaaaat ccgaggttca ctcaccagga atctcaatat
      181 ctttcatgga ctcttctttg ccaaaatcat cacaacgctg gaaactaaaa atccaatcat
      241 aagcacgctt acattcatct cacaaagcca aatcctctgg acgataattt tcgcgatact
      301 cgaaatccat cttgaacgtc cctgatcgat taacagtcac ggtgatctga aaccaccatg
      361 gctgccccat ttcttcagac agatcgcgat gctttacaag gagatcatat aacttcatat
      421 tggtagtaac atcgagtccc ttagatttat cgaccgtccc atctggaagc aagtattgag
      481 tgcgagaagc agaatttttc ttgtttggcg acattttcca aatcaaatta gcttccaccc
      541 attcatcaat tggcactgat tgatagatta aagtcgcgat tttcttgctt acttcatcca
      601 gttgatccaa tgacattgca gagcaccctc agtttacgaa tagttgacta ccacgaacac
      661 catcaatcca atactcgacc tgcaagacag aaggtactgt tgaactcgcg tcagaccaaa
      721 ccgggacaac tctgacatcg accgtctgcc ctgccctcat cgcctctgcc cattgccctt
      781 ccagcacacg gtattcacca cgattgacct gtgacatctg cgcaaccaga ttgatcccat
      841 ctccggctcc gccaaccccg tgaccgatga gatgccctcc atcgtagccc gtaccccctt
      901 cacggcccgc gcgaatctgt gcactggtgt tccggtcgag cctgtccagg ctgagccgcc
      961 cctccaccga ctccacgcgc cccagcgcat cggtccgata gacatgcccg ttgtccagaa
     1021 cgtacatcgc attcggccgg ggatggcgga gttccgcagt ccagttgccg cgcctgccgg
     1081 ccgtcgcccg gaccacactc ggccgacgag atgccaacca gtgaagcttc gatcaacatc
     1141 ttcagcccct gagcccacgc ggtcacgggt tgagcacgta cgttcgcgct actcctccat
     1201 cgccttcggc acatcgattc gatcgaacat gaagcgccgt tcatcgaacg tttcagtgta
     1261 ctcaaagcga gtccggaaac tgccgtcacg gtccacgcag acttcgatct ggtaccacca
     1321 gggttgcccc agttcctcgg ttaccttgcg atgctcgatc agcagctggg tgagctttga
     1381 tcgcaactga atatcgactt tcgtagcatc agccacgacc gatgcatcct ctcgcttgaa
     1441 gaagaactgg ttaatgctgg tttgcaggtc gggaggcagt tttgcaacat aacaaacccg
     1501 agtccagtct ggtattggtg acgcctcgtg cacgagagat gcaattttat tgatcaattg
     1561 atccaattta tataatgaca caatattccc tttttatatt catcggatat tggggtattc
     1621 gattggaggc tgcggaactc cgttaatcca agattcgacc accatatttg atggcacaga
     1681 tggatttgaa tcattccaaa ttggcgtaac ccttacatcg acagtatgcc catccgctat
     1741 atgttgtgcc cattcccgct ccatcacacg atatcggcca cgattgacct ctgacatctg
     1801 cgcgaccaga ttgatcccat ctccggctcc gccaaccccg tgaccgatga gatgccctcc
     1861 atcgtagccc gtaccccctt cacggcccgc gcgaatctgt gcactggtgt tccggtcgag
     1921 cctgtccagg ctgagccgcc cctccaccga ctccacgcgc cccagcgcat cggtccgata
     1981 gacatgcccg ttgtccagaa cgtacatcgc attcggccgg ggagaacgaa gatccgcagt
     2041 ccagttgccg cggctgccgg gcgtcgcccg caccagatcg cgtccttccg agcgcgcgat
     2101 gttcgtcaga tccgcaatgt gatctgccag ggaactcctg agcccgcccc gaacgagacg
     2161 cagggcatca tcaccgtagc cgccggatat gatcccgagc agagctatcg cccggctggc
     2221 atcgtccccg ctcaatggag atgtgccgaa gatgaactcg taaatcgaga acgttaaccc
     2281 aatgccaggc accagcccga ggacggtctc ggccgtgcgg actcgttcct ccggggaaag
     2341 gatggcctga atcgcgctct cgatttcttg aaacctctcg tccagccatt cccgcaaggc
     2401 tgaacgctcc gctggccact ccgggtccgg cggggaattc accaacgaat cgagttctgc
     2461 gcgcagttct tcagtaagcg ggtagtcacc attcccaggg cttgggttgg cgctaccgta
     2521 ttgcggcgga ccttgaacag ggttcaaatc gctatcgaga accggatacg agactcctga
     2581 accgggcgcg ccgccgaccg tcagctcaac gttatacgtg gagccatcct cgctaacccg
     2641 gattccaagc gaaaccgggg cggattgatt ttccgtaagc gccgttcgaa cccctgggtg
     2701 attgaaggct gcttccacga attgctggac gctcagtccc tgggcctcga cctgaacagc
     2761 ggcctggggg gtgacggtcg ccaccatgtg cccggcagca ttgcgaaccg aatagccgcc
     2821 atcctcgtta cgactgatgg ccaggacgtc aggggtattt tcactgcctg atcctcccga
     2881 tcccggtgat ccgtatgccg gcggctccca aggcacccga acgaacgtcg tgccgggagg
     2941 ccattggatc cgtccttcta tgacttcgac cggctgcaag gtgggttgac cggtctcggg
     3001 atcgactggg ccggacggcc acaccggaac cagttccgta gggggagcca taggaggttc
     3061 gatagggggc tctaccggta tcggagcctc ttgtccctgg tcccaatcca caggcacttc
     3121 gacaagcacc cacggcgact gatagctgtc gtcgtcaatc aactcccact cgccgcccca
     3181 cggcatctcc acccagcgaa gggtgttggt ggtcaagacc atgtcccggt cagtcgcaga
     3241 ttcatcgggg ccaagcgaat ccgatcccgt tccgcccctc cccaactcag cgccggactg
     3301 aacgtcggca aacgaggcca atcgcttggc gggcgcgccc gtcgcgccga ccggtgcttc
     3361 agcggcttgc tccgactcac cgaacacgct tacccggtca ccgcccacga ccacgacctg
     3421 atgcggcggg tagtacgccg aggcgcgatc atcgctggtg tgttgttcgt tctgcagcgt
     3481 tgccgcggac gcctccagcg cgtcgtagtg ctcgccggcg ttgacttcgc cgtaccagac
     3541 ttgggccggc atcgttgcaa ccgtctccgc ttggcccccg gctcgacccc gcgaagccgg
     3601 gagctgtcgg cgcgagcggc tcgcgctggc atcccccgtg tacagatccg gcgcttcgcc
     3661 gtacagggcg tctatcgacg ggcccactcg tcctactgta ctcatggctt ccccctggcc
     3721 gcgtttcgtg cttgcgcacg ccggcccatc tcggttcttc ttcttggttc ttgttcgcgc
     3781 ccttcgcgct aagcgaacac ggtgtgagcg agcggatctc cagaatccgc gtcgatccca
     3841 caaaacgtgc gcctgaactt atccgaatgg aaaccgaggg ggattttcaa catgtcacgg
     3901 ggaaaacatg tgaa
//
LOCUS       DAOPTR010000263         3914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_18544_length_3914_cov_3.259912, whole genome shotgun sequence.
ACCESSION   DAOPTR010000263 DAOPTR010000000
VERSION     DAOPTR010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18544_length_3914_cov_3.259912"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="PKI05_06230"
     CDS             <1..1182
                     /locus_tag="PKI05_06230"
                     /inference="COORDINATES: protein motif:HMM:NF016356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HOA51835.1"
                     /translation="VPLSLWERVGVRASDRRSPSRKPAAIRVTLTPAPSPAERARGDY
                     GIGSKPILPAGKSPLDYWQRPHEMQKDKRPCWDSDKPIRVAISACLLGQKVRYDGGHK
                     HDPFLTETLGQYVEWIPVCPEVEIGLGIPRPTLRLERRDDQICLVMPKENRDLTHTMQ
                     TFARRRVKALAEADICGYVLKKNSPSCGMERVKVYKPEGDSAGTHVGTFAGVLLEMMP
                     YLPIEEEGRLHDPRLRENWVTRIFAFHRLKNLWSQRWRLNDLVAFHTAHKYLLLAHSP
                     EHYKRLGQLVAHGKSLSREELRTRYEAEFMTGLKRLATPAKNANVLLHMLGFFKKVLP
                     DDVRAEIRGLIDDYRKGSVPLVVPVTLLAHYARRLNITYLLDQVYLYPHPKELALRSH
                     V"
     gene            complement(1362..>3914)
                     /locus_tag="PKI05_06235"
     CDS             complement(1362..>3914)
                     /locus_tag="PKI05_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51836.1"
                     /translation="TTVQDLVLNRIYEVRVSDNDAGRTITIAPVQTGNVRPGVWLVPN
                     DKKLPTSVTIPVAVFVDEEQPARQSLWEPRLRAQIAEASRFLEWFCRVRFQVVSAGSW
                     TSDNNRTTAEAVLDDFRAKVTSKEARLLIGFSSQITVPADGRFHALPRLLDTHIVLPD
                     VQATFSAQAQLMVLIHALGHFLGAVDTLQEASVMNPDYLIKATEARQPDYFDPLNVLI
                     MNIVAEDMMFRGIRQADQFSTGTKDYLSALYRFIGQRTRRRDVQIFLDALERPVPARE
                     RFLIQWWDGQQTSAPEILQWGSPDAQPEAAGRPLFDEANPVRWILDTSLQDDVTRAEA
                     WLELWAGDRLPGRVERANPAGIYEKEQLPAHLEVTPYTRVDWPSGPARATIRVLTSQV
                     KRIVWKQVTDELIAGQAFLRDGRSVPYRLVQWNGNEVRLLTSDGIQTIPLSELAELHL
                     PAVDPWEEILHQRASLVPDGKGTIVQLETNDGLIATTSLERFIIRSHGDKGLPENWYH
                     CIQPVWSLDGLWVPHRSVVWRRFFSAHEVPLYVISPASYREMCSLGGRWGFHVNRNLN
                     GTIMASGSLVFGWGFGVPSGSELVFPLAPLVSGFETKVGLDKLVGDGGCIRAAVAIKD
                     KLEHSIYESPLIVGSARVFQVTSPTTMDNDDQAQLILRIDEAHEDRPPAADPWNVRDV
                     ADWGEPILLLDRTALATALRQRRPRFIWAWSGWEPHAENGPPRIIQDLDDTSPNYPVF
                     RWLHVFDKEYAIQQTLTVPTNGRILAVFICRPRNTPEYRAEVFANGELLTSSPVPERS
                     GSNLPPPIEADVSQFAGQKTQLRILLRSEQQAGEIKAEWHAIELWKDDSSRR"
BASE COUNT          906 a         1125 c         1034 g          849 t
ORIGIN      
        1 gtccccctct ccctctggga gagggttggg gtgagggcaa gcgatcgtcg gagtccatcg
       61 agaaaaccgg cagcgattcg agtcaccctc acccccgccc cctctcccgc cgaacgggcg
      121 aggggagatt atgggatagg ctctaagccg attcttcccg cgggaaagtc tccgcttgac
      181 tactggcaac gaccacacga aatgcaaaaa gacaagcgac catgctggga ttccgataaa
      241 ccgattcggg tggccatttc tgcttgctta ttggggcaga aggtccgcta tgatggcggg
      301 cacaaacacg atccctttct gaccgagacg ctgggtcaat acgtggagtg gattcccgtc
      361 tgtcccgagg tggagatcgg tctcggtatc cctagaccga cccttcgtct ggagcgccgt
      421 gatgaccaga tatgcctcgt catgcccaaa gaaaatcgcg acctgacgca cactatgcag
      481 acctttgccc ggcgtcgcgt gaaggcgctt gcggaagcgg acatttgcgg ctacgtcttg
      541 aagaagaatt cgcccagttg tgggatggag cgtgtcaagg tttataagcc cgagggagac
      601 tcggccggga ctcatgtcgg gacctttgcc ggtgtgttac tagaaatgat gccgtatctc
      661 cctattgagg aagaaggccg cctacatgat ccccgacttc gcgaaaattg ggttacaaga
      721 atttttgcct ttcatcgcct caaaaacctt tggtcacagc gatggagatt gaatgacctc
      781 gtggcctttc acaccgccca taagtatctg ctcctggcac attcccctga gcattacaag
      841 agattaggac aattggtagc ccacggaaaa tcgctttcgc gagaggagtt acggacccgc
      901 tacgaagccg agttcatgac aggcctcaag cgcttggcaa ccccggcgaa aaatgccaac
      961 gtcctccttc acatgctggg gtttttcaag aaagtcttac cggatgacgt tcgtgccgaa
     1021 atccgtggac tgatcgatga ctatcgcaag ggaagcgttc ctctggtggt ccccgttacc
     1081 cttcttgccc actatgcccg gcgactaaac atcacgtatc ttttggacca ggtttacctc
     1141 tacccccatc caaaagaact ggccttaaga agccacgtgt gaattctttc cgacagggta
     1201 atgattcccg caagttggct gtaaactgca gcacgtgcga aaaccggcaa cagaagcgtg
     1261 acggccatcg attttggact gcgatgtcgt tctgggccaa aactctggag aatatgcgcc
     1321 tgacaaatct gtagcggcac atccctgtca ccgccgctgc gttatcgacg agacgaatcg
     1381 tccttccaca gttcgatggc gtgccactcg gccttgattt cgccggcctg ttgttcactt
     1441 cgcaacaata tcctcaattg cgtcttttga ccggcaaatt gtgagacatc ggcctcaatt
     1501 ggcggcggca aattcgatcc actcctttct gggaccggac tgctggttag caactcacca
     1561 ttggcaaaga cctcggcacg atactccggc gtattacgcg gacggcaaat aaataccgcc
     1621 agaattcggc cattagtggg aacggttagc gtctgctgaa tggcatactc cttgtcgaaa
     1681 acatgaagcc aacggaacac gggatagttt ggcgaggtgt catccaaatc ttgaattatc
     1741 ctcggcggcc cgttttcagc gtgtggttcc caacccgacc aagcccaaat aaatcgaggt
     1801 cttcgttggc gcagggccgt cgcgagggct gttcgatcaa gcaataggat aggttcaccc
     1861 cagtctgcca catcacggac attccatggg tccgccgcgg gaggtcggtc ctcgtgcgct
     1921 tcatcgatcc gtagaattag ttgggcttgg tcgtcattgt ccatagtcgt ggggcttgtt
     1981 acttggaaga cgcgagcaga acccacaata agaggtgact cgtagatgga gtgctctaat
     2041 ttgtccttga tagcaacagc cgcgcggatg cagccgccgt cgccgaccag tttatccagt
     2101 ccaacctttg tctcaaatcc gctgaccagc ggtgccaaag gaaaaacgag ttcggaaccg
     2161 gaaggcacgc caaatcccca gccaaacacc aatgaccctg aggccatgat ggtcccgttc
     2221 agattgcgat tgacgtgaaa tccccatcga ccaccgagtg agcacatctc gcggtaagag
     2281 gcaggagata tgacgtacag tggcacttcg tgagccgaaa aaaaccgccg ccacaccacc
     2341 gagcgatggg gtacccaaag cccatcgagg ctccacaccg gctggatgca gtgataccaa
     2401 ttctctggaa ggcccttgtc cccatgagaa cggatgatga atcgttccaa cgatgtggta
     2461 gctatcaagc catcgtttgt ctcaagctga acaattgtgc ctttgccatc gggcaccaag
     2521 ctagcccttt ggtgcaagat ttcttcccac ggatcaaccg cgggaagatg aagctccgcc
     2581 aactcagaca gtgggatcgt ctgaattcca tcactcgtga gcagccgtac ttcattcccg
     2641 ttccattgca cgaggcggta tggcacgctc ctgccatcac gcaagaacgc ctgaccggca
     2701 atcagctcat ccgtaacctg cttccagacg attcgtttca cctgagaagt caacacgcga
     2761 atcgtcgccc gagccggtcc ggaaggccaa tcgacacgag tataaggcgt gacttccaga
     2821 tgggccggga gctgttcctt ttcgtagatc ccagccgggt tggccctctc aaccctccca
     2881 ggaaggcgat cgccggccca aagttccagc caggcttccg cacgggttac gtcatcctga
     2941 agcgatgtat cgagaatcca gcgcacggga ttggcttcgt cgaacaatgg ccgacccgca
     3001 gcttctggtt gcgcatcggg acttccccat tgcaatatct ccggagcgct cgtttgttgt
     3061 ccgtcccacc actggattaa gaaccgttcc cgtgccggaa ccggtctctc taaagcgtcc
     3121 aagaagattt ggacgtcccg tctccgcgtc ctttggccaa tgaaacgata aagtgccgac
     3181 agataatctt tcgttcctgt tgagaattga tccgcctgcc tgatgccacg gaacatcata
     3241 tcctcggcca cgatattcat aatcaggaca ttaagcggat caaaatagtc cggttggcgg
     3301 gcctccgtcg cctttattag gtagtccgga ttcataaccg aggcttcctg aagggtgtcc
     3361 accgcgccga gaaagtgtcc caaggcatgg atcagcacca tcaactgagc ctgggcggaa
     3421 aacgtcgcct ggacatcggg caacacgatg tgtgtgtcga gcagccgagg taaggcatgg
     3481 aaacgaccgt ccgcgggtac cgtgatttgg ctactaaacc caatgaggag cctcgcttcc
     3541 tttgaagtga ccttcgctcg aaaatcatca aggactgctt cagcggtcgt gcgattattg
     3601 tccgaagtcc agctccccgc actcacaact tggaaccgaa cacgacaaaa ccactccaga
     3661 aagcgcgaag cttcggcgat ctgggcgcga agccgaggct cccacagcga ctgccgggcg
     3721 ggctgctctt cgtcaacgaa aaccgcgacg ggaatcgtca cggatgttgg cagcttcttg
     3781 tcgttgggga ccaaccaaac gccgggacga acattacctg tttgcaccgg tgcgatcgtt
     3841 atggtccttc ccgcgtcatt atccgaaacg cggacttcat aaatccgatt gagcaccagg
     3901 tcttgaaccg ttgt
//
LOCUS       DAOPTR010000264         3910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_18566_length_3910_cov_4.102983, whole genome shotgun sequence.
ACCESSION   DAOPTR010000264 DAOPTR010000000
VERSION     DAOPTR010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18566_length_3910_cov_4.102983"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(661..2793)
                     /locus_tag="PKI05_06240"
     CDS             complement(661..2793)
                     /locus_tag="PKI05_06240"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOA51837.1"
                     /translation="MFRCHLHSKALLYVSILFLGGVATTGLIFAQPRPEWKPLGPLPG
                     DAMLADYFQVEVGKLQAACLADIKTLEDWQARCEEYRRQLREMLGLDPWPKKTDLKAT
                     ITGMLERDDFRVEKLHFQSMPGLYVTANLYLPKNITKPVPAVLYLCGHAQVKIDNVSY
                     GNKAHYHFHAVWFARHGYACLVLDSLQLGEIEGIHHGTYRYGMWWWNNRGYTPAGVEA
                     WNCVRALDYLQSRPEIDGERIGVTGRSGGGAYSWWIAAIDPRIKAAVPVAGITDLQNH
                     VIDGCVEGHCDCMYIVNTYRWDYPMVAALVAPRPLLIDNGDHDPIFPEDGVRRVYEQA
                     RKIYQLYGAEDKIAIFITTAGHDDIPTIQEAAFRWLDRHLMGKERDTYDPVEKVLTPQ
                     ELKVFETLPGDQLNTTIHEHFVPAAPVVFPQNQDEWKRMQTEWLKGIREKCFRGWPGD
                     LPPATLRDAVSVVRDGVRLTRAKLDVQDKITLPVYRLELAAGSVRRAIVEVLDQTDWQ
                     EWLKGLKAVFADDLTDDLVTDEDKALAGDESAWKEIKARLEAERGTALVLLMPRGVGP
                     TRWNAEAKKQTQIERRFMLICQTSDGMRVLDILRGLQAIRSSGNLPEKSMRVRAKGKM
                     GVLACYAAMFETELALELDQIPLTHRDGPYILNISKVLEVPQAVFLAAQTNPARLTTD
                     RPEAWQPALEAAQRLGWPESQLRIIKRE"
     gene            3033..>3910
                     /locus_tag="PKI05_06245"
     CDS             3033..>3910
                     /locus_tag="PKI05_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51838.1"
                     /translation="MKMLNLRESVSGCGCCLSRRRFLASCAACAAGAAISQPMGRKIL
                     ASEAKPMRIRILYALHGPVQEVPDWPNIGFDFRPVMERIEKTLKGACPNMEFVSAMAN
                     GPEAAAKIVEEDKANPPDGYIVYQMNCWNRVVQTVADTGKPVLYLDFQFAGSGGFLVY
                     TAAFLRQNRPNVAFVGSSNMEDHVAAVKCFEIIKSGGSVADFVAAVSKTRKDRTPAPG
                     DMTCKPDELKCLSVEETLRQLREKKILAVGGGWGGPRFFEAIQKELGVPVISIGFEEL
                     NEAWKQADKDQAKEIA"
BASE COUNT          860 a         1085 c         1088 g          877 t
ORIGIN      
        1 gaagcggctc ctcatgttcg gagtgtgttt tcggaccttc attgtgtgtt ctcctttcgt
       61 gaaagctacc aaactcacca aagaccgttc ccaatacttt ctgcacctct tgctttttgt
      121 tgcgagcgtc atttgcgagt ttgctccaac gcatccggcg ttggccctcg atagttccgc
      181 tcgcatggtc cctggaagag ctcttccaca tttgaaccac acttgaagat acgaatgctg
      241 cgttaagatc aagttaggaa acggttaaga aatggttaaa aatgcgagca ttaattgaga
      301 ctcaatatca tgacaccccg ctcacgaacc cggcgactca accacctagg tcgcgagact
      361 ttctctgtgc tgtgcgaagg tccttgacaa aaaccggtct tttcgctgac aacactccgc
      421 cctcatcgct tcgctcagtc tgcctagcgc gcgggcgagg gctgtgatga cgaggacgcg
      481 gcgtaagttc cagatgagtg gcagtggcac tctgcaaaga aagcaccgca atagcagcgt
      541 ggcagccctc ggcgaccgct ttgaccggtc gcgggcggtg atattgggca gctcggttgc
      601 ctggcttgta aggcgatccc ctgatctcaa ggcgtcatac gacgcggcgc ggagcgcggc
      661 tcactcccgc tttatgattc gcaactgcga ttcgggccag cctaagcgct gggcagcctc
      721 cagtgcaggt tgccaggctt caggtcggtc ggttgtcagg cgggcaggat tggtctgcgc
      781 cgccaaaaat acggcctgcg gcacctccaa cacttttgaa atgttgagga tataggggcc
      841 atcacggtgt gtgagaggga tttgatctag ttccaaggcg agctctgttt caaacatcgc
      901 ggcatagcag gcaagaaccc ccatcttgcc cttggcgcga accctcatgg acttttctgg
      961 cagattgccc gaggagcgaa tggcctgcaa tccgcgaagg atatccagta cccgcatccc
     1021 atcgctggtc tggcaaatga gcatgaaccg gcgttcgatc tgtgtctgtt ttttagcttc
     1081 ggcgttccat cgagtggggc ccacgccgcg tggcatcagc aggacgaggg cggttccccg
     1141 ttctgcctca agccttgcct tgatctcttt ccatgcgctt tcgtcaccgg caagggcctt
     1201 atcctcatcc gtgacgaggt cgtcggtcag atcatcggca aagaccgcct tgaggccctt
     1261 gagccactcc tgccaatctg tctgatcaag gacctccaca attgcacggc gtacggagcc
     1321 ggccgccagt tccaagcgat agacgggcag ggtgatcttg tcctggacat cgagttttgc
     1381 tcgcgttaac cgaacaccat cccggaccac actcacggca tcacgcaacg tggcgggagg
     1441 aagatcgccg ggccagcccc ggaagcactt ttcgcgaatt cccttcagcc attccgtttg
     1501 catgcgcttc cattcatcct ggttttgcgg gaagacgacc ggggccgctg gaacgaaatg
     1561 ttcgtggatc gttgtgttga gctgatctcc cggcaatgtc tcaaaaactt tcagttcctg
     1621 aggcgtgagg accttctcca ccgggtcgta cgtatcgcgt tccttgccca tcaaatggcg
     1681 atccaaccag cgaaaggcag cctcctggat agtcgggatg tcatcgtggc cggcagtggt
     1741 gatgaagatg gcgatcttat cttcagcgcc ataaagctgg taaatctttc gcgcctgttc
     1801 ataaacccgt cgcacaccgt cttcgggaaa aatgggatca tggtccccat tgtcgataag
     1861 gagcggtcgg ggcgcgacca aggccgccac cattgggtag tcccatcggt aggtgttgac
     1921 gatgtacatg cagtcgcaat gtccctccac acacccgtcg ataacgtgat tttgaaggtc
     1981 tgtaatgccc gccacgggga cggccgcctt aatgcgggga tcgatcgccg cgatccacca
     2041 actgtaggca ccaccaccgc tgcggcccgt gacaccgatc cgttccccgt caatttctgg
     2101 gcgggattgc aggtaatcca atgcccggac acagttccac gcttccaccc cggccggtgt
     2161 gtacccgcga ttattccacc accacatccc gtaacgatag gttccgtgat gaattccctc
     2221 gatttcgccc aattgcaggc tatctagcac cagacaagcg tatccgtgac gggcaaacca
     2281 cacggcgtgg aagtgataat gggccttatt accgtaactg acgttgtcaa tcttgacttg
     2341 ggcatgacca cacagataaa ggacagccgg tacgggcttg gtgatattct tgggcaaata
     2401 gagattggcc gtgacataaa gcccgggcat ggattgaaaa tgcaacttct ccacgcggaa
     2461 atcatctcgc tccaacatgc cggtgatcgt ggctttcaaa tctgtctttt tcggccaggg
     2521 atcaaggcca agcatttccc gcaactggcg tcgatactcc tcacagcggg cttgccagtc
     2581 ttcgagggtc ttgatgtcgg ccagacacgc tgcctggagc ttgccgactt ccacctgaaa
     2641 gtaatccgcc aacatggcat cgccgggcaa gggaccaagg ggtttccatt ccgggcgcgg
     2701 ctgagcaaag atcaatcctg tcgtggctac gccgcccaaa aagaggatgc ttacatacag
     2761 gagcgccttg gaatgaagat gacaccgaaa catgatttcg acctcccgct gacctaccag
     2821 ttaacctgtc gccgatcaca aaaccacaga tattgaaaca ggttcacagg gccgaagcaa
     2881 gtcggttgcg ttgaatagtt gactaaagca atcaagccaa tcatcttaat gcttgagttt
     2941 accggcagga tgggataccg tgatgatgtg tttacggtag agcttccgtt tgggttcccg
     3001 ttggtcaagt tccaagtatc gagggtggta agatgaagat gctgaatctt cgagaatcag
     3061 tttcgggttg cgggtgctgc ctttcacggc gtcgtttctt ggcgagctgc gctgcctgtg
     3121 cggccggggc ggcgatatct caaccgatgg ggcgaaagat tctggccagt gaggccaagc
     3181 cgatgcgaat tcgcatcctt tatgcccttc acggtccggt ccaggaggtg cctgactggc
     3241 cgaacattgg cttcgacttc cgcccggtga tggaacggat cgaaaagaca cttaagggtg
     3301 cctgcccgaa tatggaattt gtctccgcga tggccaatgg gcccgaagcg gcagctaaga
     3361 tcgtggagga agataaggct aatccgccgg acggctacat tgtctatcaa atgaattgct
     3421 ggaaccgtgt tgtgcaaact gtggccgaca cgggcaaacc cgtcctttac ctcgacttcc
     3481 aatttgcagg cagcggcgga ttcctggtct atacggcggc cttcctgcgg caaaataggc
     3541 caaatgtggc ttttgtcggc tcatccaaca tggaagatca cgtagcggcc gtcaagtgtt
     3601 ttgagatcat caaatcgggc gggtcagtcg ccgactttgt ggcggccgtg agcaagacgc
     3661 gtaaagacag aacacctgct cccggcgaca tgacctgcaa gccggatgaa ctgaagtgtc
     3721 tctcggtcga ggaaaccctc cggcaacttc gggagaagaa gattctagcc gtcggcggtg
     3781 gatggggagg ccctcgcttc tttgaagcca tccaaaaaga attgggagtt cccgtaatca
     3841 gcattgggtt tgaagaactt aacgaggcgt ggaagcaggc tgacaaagat caggccaagg
     3901 aaatcgcaca
//
LOCUS       DAOPTR010000265         3881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_18755_length_3881_cov_3.145321, whole genome shotgun sequence.
ACCESSION   DAOPTR010000265 DAOPTR010000000
VERSION     DAOPTR010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18755_length_3881_cov_3.145321"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PKI05_06250"
     CDS             <1..572
                     /locus_tag="PKI05_06250"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOA51839.1"
                     /translation="AYLMPVLSETGNTPLAYRLLLNDTCRAWGFSIKHGATSIWERWD
                     GWTPEKGFQDPGMNSFAHYSFGAVGRWMFQYVAGIDTDGPGFRRLILKPHVAEGLDWV
                     EARYGSICGDIRSRWWYEGDNKTWHWRVVIPPNTTATIYVPSKNPQQVKVMAGKEVAK
                     FVRQEDDYAIFQAPAGNYEFSVLPPDQKD"
     gene            578..1396
                     /gene="proC"
                     /locus_tag="PKI05_06255"
     CDS             578..1396
                     /gene="proC"
                     /locus_tag="PKI05_06255"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOA51840.1"
                     /translation="MLNKRIGMIGAGQMALALAAGFVRKGLVPPEYILASDPLENARQ
                     RFQEVTGARVTDNNLEVVRTKDIIILAIKPQVISKVFEELQGRISPEKLVISIVAGVT
                     LETLQRALGENARIIRAMPNTPALVGSGITAYAVGKNIQPADVQTVEAILSAVGVTVQ
                     VEERLMNAVTAVSGSGPAFVYLFIEALVDAGVRHGLPRELARQLVVHTVRGASEMVIQ
                     SGEHPALLRDRVTSPGGTTIAGLAALETNGFRGAVFAAIEAAVQRAEELSRGSR"
     gene            1492..>3881
                     /locus_tag="PKI05_06260"
     CDS             1492..>3881
                     /locus_tag="PKI05_06260"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOA51841.1"
                     /translation="MSSVEYSHNGPRNVIICLICAIALVFGATGFGQEVCRQSARAIP
                     IVADVDVAVIGGSTGAVAAAVSAANEGARVFLAAPQPYLGDDMTATLRLWLEPDEKPT
                     TDLARAVYSDPLRPAPEANRARYGFRYQLDVPSDPKHRDTSPPSMLTDGKFMHAPTES
                     VQYNSDATITADLETPQVVDRVNLIFFWRPSGEPGVGFKVAGIEVSISDNGKEWVPIG
                     KADVTPPQAASGDPPLSVRVDVGQKKARFVKVFVRKAPDVERMLLGELEILGPAQEPV
                     EREQAKYLPPRPLHVKQLLDKVLLDANVQYLYSCYPTDVVRDASGRVCGVVIANRAGR
                     QVVLAKAIIDATDRATVARLAGAKFSKYPSGTHVFTRVVIGGEPVTGEGLEHRVIDPP
                     FVGQFPNRARTSSGIFKIIEYKLRLPMPDDSWRSWAIADQKARSLTYHPEQQFTSDFL
                     FEVPPDSVVAQEEAKGPWNDIASLPLGAFAPANVQGVWILGGCAAVSREQAEKLLRPV
                     HLMELGERLGREIARIASSTPKPESPVVAVNPIQGNPIAGDVGEFLYGLRPVKKYPTI
                     PQPETTIPVLGKYDVVVIGGGTGGAAAGIGAARQGAKTLVVEYLTTLGGVGTAGAISS
                     YYWGNRTGFTATVDEGRASWVIEQKAEWWRSEILKAGGEIWMNCLGCGAYKDNGRVKG
                     AIVVTPFGRGVVLADVVIDSTGNADIAAAAGAETMYVDDTEPAMQGTGLPPRELGASS
                     RNTDFTITDETDLMDVWHVFIYSKLKYSSAFDQGRLVDTRERRRIVGEFTMTLLDQV"
BASE COUNT          853 a         1000 c         1153 g          875 t
ORIGIN      
        1 ctgcatattt gatgccggtg ctctcagaaa ccgggaacac gccgttggct taccgcctgt
       61 tattaaatga cacgtgtcgg gcctggggat tctcgatcaa gcatggtgcg acatcgattt
      121 gggaacgctg ggacggttgg acaccggaaa aaggatttca ggaccctggc atgaactcct
      181 ttgctcatta ttcgtttggg gctgtcgggc gatggatgtt ccaatatgtc gccggtatcg
      241 acacagatgg cccgggattt cgacggttga ttctgaagcc gcacgtagcc gagggtttgg
      301 attgggtcga ggcccgctat ggttccattt gcggcgatat tcgcagccgg tggtggtatg
      361 agggcgataa taagacgtgg cattggcgag ttgtcatccc acctaatacc actgccacca
      421 tctatgtgcc ttccaagaat cctcaacagg tgaaagtcat ggccggtaag gaagttgcca
      481 aatttgtccg acaagaagac gattatgcca tctttcaggc tcctgctggg aattatgagt
      541 tcagcgtctt gccgccagac caaaaggact gagtcacatg ttgaataaga gaatcggcat
      601 gattggcgcg gggcaaatgg ctctggcctt ggcagcaggg tttgtccgga agggtctcgt
      661 tccgccggag tacattctgg cttccgaccc tctggagaac gcccggcaac gattccagga
      721 agtgactgga gcacgagtga cggataacaa tcttgaggtc gtgcgaacca aagatatcat
      781 tatcctggcc atcaagcccc aagtcatttc aaaggttttt gaggaactac aaggccggat
      841 aagtccggaa aaactggtga tttccatcgt ggcaggcgtg actttggaga cccttcagcg
      901 ggcgttagga gaaaacgccc ggatcatccg ggcgatgccg aatacacccg cgctcgtagg
      961 atctggtatt accgcctacg ccgttggaaa gaacattcaa ccggcagacg tgcaaaccgt
     1021 cgaggcaatc ctcagtgcgg ttggcgtcac cgtccaagtg gaagagcgac tgatgaacgc
     1081 tgtgacggcc gtttccgggt ctggaccggc ctttgtgtat ttgtttattg aagcattagt
     1141 ggatgcaggc gtccgacatg gtttacctcg tgagctggct cgccaattgg ttgtccatac
     1201 ggttcgtggg gccagcgaga tggtgatcca gtcgggggag catcctgccc tgttgcgtga
     1261 ccgggtgaca agccccgggg gaaccaccat tgcggggcta gcagcactgg agacaaacgg
     1321 cttccgcggg gcagtgttcg ccgcaatcga ggccgctgtg caacgggctg aagaattgtc
     1381 tcgaggttcg cgctagcgat agtattagaa tgagttgtgc aaaaactgcg acgagcgata
     1441 atcactacaa gaaaaggcca ttcgtcctgt gaacgctgaa aggagggtgt tatgtcttcc
     1501 gtcgaatatt cgcataacgg acctcggaat gtaatcatct gcctgatttg cgctatcgct
     1561 ttggtcttcg gcgcaactgg ttttgggcaa gaagtctgtc gtcagtcggc gcgggccatt
     1621 ccgatcgtcg ctgacgtgga tgttgccgtc atcgggggaa gtacaggtgc ggtagcggcg
     1681 gcagtcagtg cggcaaatga aggcgcccgc gtctttttgg ccgcaccgca gccctacctg
     1741 ggcgatgata tgacggcaac cctccgtttg tggctggaac ccgacgaaaa gccgacaacg
     1801 gacttggcga gggcggtgta ttccgaccca ttgcgtccgg ctcccgaagc caaccgtgca
     1861 cgttacggat tccgatatca gttggatgtc cccagcgatc caaagcatcg agacaccagc
     1921 ccgccgtcga tgctcaccga tgggaaattc atgcacgccc ccaccgaaag tgtgcaatac
     1981 aacagcgatg ccacgattac ggccgacttg gaaacaccgc aggtggtcga tcgcgtaaat
     2041 ctcatcttct tctggcgacc atcgggtgaa ccgggtgtgg gattcaaggt cgccggtatt
     2101 gaggtgagca ttagtgacaa cgggaaagag tgggtaccca tcggcaaagc cgatgttacg
     2161 cctccgcagg ctgccagtgg tgatccaccg ctttccgtca gggtcgatgt tggccaaaaa
     2221 aaggctcgct ttgtgaaagt cttcgtccgc aaagcacctg acgtggaacg aatgctcctc
     2281 ggggaactgg agattctcgg cccagcccaa gagccggttg aaagggagca agccaagtac
     2341 cttcccccgc gtccgctcca tgtcaaacaa ctcttggaca aggtgctttt ggatgccaac
     2401 gtccaatacc tctacagttg ttatcccacg gatgtggtac gcgatgcgtc tggccgggtt
     2461 tgcggcgttg tcattgcaaa ccgcgccggt cgtcaggtcg tcttggccaa ggctattatc
     2521 gacgccactg accgggctac agtcgcgcgt ctggcaggag caaagttctc taaatacccc
     2581 agtgggacgc atgttttcac tcgggtggtt atcggcggag aacccgtaac cggggaagga
     2641 ttggaacatc gtgtcatcga tcccccattc gtaggtcaat ttcccaatcg tgcccgcacg
     2701 tcgagcggga tatttaaaat catcgagtat aaactccgtc taccaatgcc tgacgattcc
     2761 tggcggagct gggccattgc cgatcagaag gcgcgatcac tcacgtacca ccccgagcaa
     2821 caatttacat ctgacttctt gttcgaagtt ccccccgatt ccgtggtcgc tcaagaggag
     2881 gcaaaaggcc cttggaatga tattgcttca ctgccattgg gtgcttttgc ccctgcaaac
     2941 gtacaggggg tgtggatttt ggggggatgt gccgctgtgt cgcgagagca ggcagaaaag
     3001 cttttgcggc cagttcacct tatggaattg ggcgagcgat taggccgaga gatagctcga
     3061 attgcatctt ctacacccaa acctgaatcg cccgttgtgg ctgtgaaccc aatccagggg
     3121 aatcctattg ccggcgacgt gggcgagttt ctctacggcc ttcgtcctgt taagaaatac
     3181 ccaacgattc cccaacctga gaccacgatt cccgttttgg gtaaatatga cgtggtcgtc
     3241 attgggggtg gaacgggcgg tgccgccgcc ggtattggcg ctgctcgcca aggggccaaa
     3301 accttggttg ttgaatattt gaccacactc ggaggcgtcg gtaccgctgg ggctatctcg
     3361 tcctattact ggggaaatcg taccggcttc acagccacgg ttgatgaggg acgagccagt
     3421 tgggtcattg agcaaaaggc ggaatggtgg aggagcgaga tccttaaggc cggtggtgaa
     3481 atttggatga attgcctagg gtgcggagct tacaaggaca acgggcgagt taaaggtgcc
     3541 attgtggtaa cacccttcgg gcgtggtgtg gttttggctg atgtcgttat cgactccacc
     3601 gggaacgcgg acatcgccgc ggcagccggg gccgaaacca tgtatgtcga tgataccgaa
     3661 cccgcgatgc aagggaccgg cttaccgccc cgtgagttgg gtgctagttc acggaacacc
     3721 gatttcacca tcacggatga gactgatctg atggatgtct ggcacgtgtt tatctattca
     3781 aagctcaaat acagcagcgc ctttgaccag ggccgcctgg ttgatactcg tgaacgccgg
     3841 cggatcgttg gtgaattcac aatgaccctt ctcgatcaag t
//
LOCUS       DAOPTR010000266         3878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_18769_length_3878_cov_3.123725, whole genome shotgun sequence.
ACCESSION   DAOPTR010000266 DAOPTR010000000
VERSION     DAOPTR010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18769_length_3878_cov_3.123725"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PKI05_06265"
     CDS             <1..734
                     /locus_tag="PKI05_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOA51842.1"
                     /translation="NTAQEKLLQAEIIDVIAQARQKWTLRRYFPLLLRGAWVTTYLSV
                     LSMLLAMILGLPIALARLFGSLPIRWLAVAYVEFFRGIPILLLLFFLYYGLPTIADQY
                     GLPFTFRLSPITAAILGFGLNYAAYEAEIYRAGIRSIPSGQWEAAASLGMGRVLTFRR
                     IILPQAIRVILPPMTNDFIALFKDTSIVSIVAVVELTKQYQILAKSSLKYLEIGLVTA
                     LLYLILSVPLGALSRHLEARWGKGQ"
     gene            731..1285
                     /locus_tag="PKI05_06270"
     CDS             731..1285
                     /locus_tag="PKI05_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOA51843.1"
                     /translation="MNKDEFSIEVEDLCKRFGHRTILHKVAFRVDRGETVAIIGPSGA
                     GKSTLLRCLNGLCNFDGGTIRVGSLVLRSGRRPAPFATLRQIRQRLGMIFQDFQLFPH
                     LTVLQNVTEAPIHVLGVARRDAERQAQALLDRVGLADHIHAFPQQLSGGQKQRVAIAR
                     ALAMNPFGLLCDEITSALDPELKA"
     gene            1286..1489
                     /locus_tag="PKI05_06275"
     CDS             1286..1489
                     /locus_tag="PKI05_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51844.1"
                     /translation="MLAVLEDLRRDGMTLILVTHEMAFAQRVADRIIVLVDGEIVEDG
                     PPARVFRTPEHPRTTRFLEQVLL"
     gene            1509..2645
                     /locus_tag="PKI05_06280"
     CDS             1509..2645
                     /locus_tag="PKI05_06280"
                     /EC_number="1.17.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009095663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin/ferredoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOA51845.1"
                     /translation="MRKNSTRAVRVGSVTFGGGNPIVVQSMCATKTQDVAATVAQVEQ
                     LRQAGAGIVRIAVDSPADAQALVAIREQTSANLSVDLQENYRLVTQVAPYVNKVRYNP
                     GHLYHHQTNVPWQDKVKFIADVAAQYGCALRIGVNCGSIDPAKREKFPSEDKISPMLE
                     SAWEHCELLDRLGFSQYCVSLKESDPQMVIEANRRFAAARPDVPLHLGVTEAGMPPEG
                     IRKTRAALIPLLAEGIGDTIRVSLTLPNERKHEEVLAGLEIVADVAAGRVTVELWTRD
                     GLNIVSCPSCSRVENAAFVQLTEKVRELTAYAASYPITIAVMGCRVNGPGETDDADLG
                     LWCGPKYVNLKKGPVTLGSFTYDEILPRLKQELDALIAERFPVT"
     gene            2659..2880
                     /locus_tag="PKI05_06285"
     CDS             2659..2880
                     /locus_tag="PKI05_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51846.1"
                     /translation="MTVCLFTDFAYVPRWCSLAIVVRPFLCNRGADTDGDITPTEGGL
                     PYGPVEVTIIGGDRDEGYVTIKVFGPFGR"
     gene            complement(3380..>3878)
                     /locus_tag="PKI05_06290"
     CDS             complement(3380..>3878)
                     /locus_tag="PKI05_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51847.1"
                     /translation="SKVLNSFGHPVPVVAGQLQRSGSQARAKVLETQFTDEADVYRID
                     ITSAYNVPALKSLVREFRYERQGRGRLIVTDRVEMSSPQSFEEALITYAPWRRVADGI
                     WIVGEGRGAVRVELTMEGQSFVASEEEIDEDVRYNKKPVRLAAKVKEPIASGTVVIRI
                     EPVEK"
BASE COUNT          739 a         1095 c         1168 g          876 t
ORIGIN      
        1 ggaacaccgc tcaggaaaag ctgctccaag cggagatcat tgacgtcatt gcccaggccc
       61 gccaaaagtg gacgttgagg cgatacttcc cgctcttgtt gcgaggagcc tgggtgacaa
      121 cctatctgtc ggtgctgagc atgctgctgg ccatgatcct gggcctgccg atcgccctgg
      181 cccgactttt tggttcgtta ccgatccgtt ggctggccgt tgcgtatgtg gagtttttcc
      241 gcggtattcc catcttgctc ctgctgttct tcttgtacta cggattgccc acgattgccg
      301 accagtacgg gctgccgttt acctttcgtc tgtcacccat cacggcggcc attttgggat
      361 ttgggcttaa ttacgccgct tacgaggccg agatttatcg tgcaggaatt cggtccatcc
      421 cttcgggaca gtgggaggcg gcggcctcgc tggggatggg gcgcgtgtta accttccgcc
      481 ggatcatcct tccccaagca attcgcgtga ttctcccccc gatgacgaac gactttattg
      541 cgctttttaa ggatacgagc atcgtcagca ttgtcgccgt agtagaatta accaagcagt
      601 atcaaatttt agcgaaatcc agtctaaaat atttggagat cggcttggtg acagccctgc
      661 tgtacctgat cctttcggtt ccgttgggtg ctctttcccg gcatctcgaa gctcggtggg
      721 gaaaaggcca gtgaataaag acgagttcag catcgaagtt gaagacctgt gcaagcggtt
      781 cggtcatcgg acgattctgc acaaggtggc gtttcgcgtg gatcggggcg aaacggtggc
      841 catcatcggc ccaagtgggg ctgggaaatc gaccctccta cgttgcctca acggcttgtg
      901 caattttgac ggcgggacaa tccgcgtggg ctcgcttgtt ttgcgttccg ggcgccgacc
      961 ggcccccttc gctacgcttc gtcagattcg ccagcgcctg ggcatgatct ttcaggattt
     1021 ccagttgttt ccccatctga cggtcctcca gaatgtcacc gaggccccca ttcatgtgct
     1081 gggcgtggcc cggcgggatg cagagcggca agctcaggcc ctcctcgacc gcgtggggtt
     1141 ggcggatcat attcatgcct ttccgcaaca gctttccggg ggacaaaagc agcgggtagc
     1201 cattgcccgc gccttggcga tgaatccctt tggcctgctc tgtgacgaga tcaccagcgc
     1261 cctcgatccg gaacttaagg cgtaggtgct ggcggtcctc gaggaccttc gccgcgatgg
     1321 aatgactctc attttggtaa cacacgaaat ggcgtttgcc cagcgggttg ccgatcgtat
     1381 catcgttctg gttgacggcg agattgtgga agatggtcca ccggctcgcg tcttcaggac
     1441 tcccgaacat ccccgcacaa ctcgctttct ggaacaggtg ttgctttagc cccgtgtgaa
     1501 aggtctctat gcggaagaat tcgacccgcg ccgtgcgtgt cggctctgtg acgttcggcg
     1561 gcggaaatcc gatcgtggtt cagagtatgt gtgccacgaa gacgcaagat gttgcggcga
     1621 cagtggccca ggttgaacaa ttgcgccagg cgggcgccgg tattgtgcgg atcgccgtgg
     1681 atagcccggc ggatgcgcag gccttggtgg ccattcggga gcagacctcg gccaacctgt
     1741 ccgtggacct acaagagaat taccgtctcg tcacccaggt ggcaccgtat gtcaataagg
     1801 tccgctacaa tccgggccac ctctatcatc accaaacgaa tgtcccgtgg caggataagg
     1861 tgaaattcat cgccgatgtt gcggcccagt atggctgtgc ccttcgcatt ggggtgaact
     1921 gtggatcaat cgatccggca aaacgcgaga agtttccgtc cgaggacaag atctctccga
     1981 tgcttgaatc ggcatgggaa cactgtgagc ttctcgatcg gctagggttt tcacagtatt
     2041 gcgtgtcgct caaagaatcg gatcctcaga tggtcatcga ggcgaaccgg cggtttgctg
     2101 cggcccgacc ggatgtgccg ttacacctcg gtgtgacgga ggccggcatg ccgccggaag
     2161 gaatccgcaa gacgcgtgcc gcgttgattc cccttttggc agaaggaatc ggtgacacca
     2221 ttcgcgtctc tttgacgtta cccaacgaac gaaagcatga ggaagtcctg gccggtttgg
     2281 agatcgtggc cgatgtggct gccggacggg tcacggtgga gctttggaca cgggatgggc
     2341 tgaacatcgt cagttgtccg agttgttccc gcgtcgaaaa tgccgccttt gtgcagttga
     2401 ccgaaaaggt ccgcgaattg acggcttatg cggcgagcta tcccatcacg attgccgtca
     2461 tgggctgccg cgtgaacggt cccggcgaga ccgacgacgc cgatctggga ctgtggtgtg
     2521 gtccgaagta tgtgaatctg aaaaaaggcc cggtcacgct agggagcttc acatacgatg
     2581 aaatcctgcc acgactaaag caggaactgg acgccctgat cgccgagcgg ttccccgtga
     2641 cataacccgg ccagtgggat gaccgtgtgt ctctttaccg atttcgccta tgtgccgcgg
     2701 tggtgcagcc ttgcgattgt tgttcggcct tttctgtgca accgcggtgc cgacacggat
     2761 ggggatataa ccccaaccga gggtggattg ccttacgggc ccgttgaggt tacgatcata
     2821 ggtggcgatc gtgatgaagg ctacgtgacc atcaaggttt ttggcccatt tggccgatga
     2881 acttggatgc cggcacaggc cgcgcacgat tgcgaaaaaa gcactcgacc gagcgtggtc
     2941 agtgatggat cgacggtggg ctgataccgt gcccgatggg agccgtcccc gaacaagtat
     3001 ccagcacgag cgtggagcca tcgtgtgaga aacctcgggt ttctacggtg gatgacgcgg
     3061 ttgccggatc acggcgcccc cgtgaaagtc gggcctacct gttcgccatc actccgctcg
     3121 cgagtgcttt ttttatgcgc aggcccagtg ccgagcggtc ccgacccgcg ctatctttcg
     3181 gagcgggcac tctcggcaac ggacgcgatg attgcgctta cagcctctct gaaaaagtcc
     3241 ccctctccct ctgggagagg gttggggtga gggcaagcga acgtcggaat tgattgagaa
     3301 aaccggcagc gattcgagct accctcaccc ccgacccctc tcccgccgaa cgtgcgaggg
     3361 gaaatcatgg gataggctct tatttctcca ccggttcgat gcggatgaca accgttccgc
     3421 tggcgatcgg ttccttgacc ttggcagcta aacggaccgg tttcttgttg tatcgcacgt
     3481 cctcgtcgat ctcttcttcg ctggccacga aggactggcc ttccatggtc agctcaacgc
     3541 ggacggcacc gcggccttcg cccacaatcc aaatcccgtc ggcaactcgt cgccaagggg
     3601 cgtaggtgat gagggcctcc tcaaaagatt gcggtgacga catctcaacg cgatcggtaa
     3661 caatcaggcg accacgtccc tggcgttcgt atcggaactc gcggaccagg cttttcaggg
     3721 ccgggacatt gtaggccgac gtgatgtcga tccgataaac atcggcttca tcggtgaatt
     3781 gcgtctcgag gaccttggcc ctggcttggc ttcccgatcg ttggagttgc ccggcaacca
     3841 cggggacggg atggccgaaa gagttgagca ccttgctc
//
LOCUS       DAOPTR010000267         3862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_18883_length_3862_cov_3.580772, whole genome shotgun sequence.
ACCESSION   DAOPTR010000267 DAOPTR010000000
VERSION     DAOPTR010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18883_length_3862_cov_3.580772"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..988
                     /locus_tag="PKI05_06295"
     CDS             104..988
                     /locus_tag="PKI05_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA51848.1"
                     /translation="MIAQATTEGTISSPSSKDPVLLEVEQLTVAFGRQVVLRGINVQI
                     RRGETVAILGESGCGKTVFLKTLVALQRPTKGRVLFDGRDLFQLRPHELAEQRIRMGF
                     VFQQAALFDSMTIGENIAFPLRQHTRLTEDEIYEVVKARLLEVGLPENVLRKKPGEIS
                     GGMRKRVGLARALALEPELMLYDEPTTGLDPIMSDVINELILRTRRQHPVTSVIVTHD
                     MRTATKVADRIIMLYPLTRLKPGESQILYDGPASEIESAADDRVIQFVRGEARERLME
                     MAREESENAVEIGGAAHG"
     gene            981..2159
                     /locus_tag="PKI05_06300"
     CDS             981..2159
                     /locus_tag="PKI05_06300"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOA51849.1"
                     /translation="MDERLMQFRIGVMVLASILIAAILIVTFGEWRWPFPAEYTVYIR
                     FPEAPGVAERSPLRKSGIVIGYVRSVELTPEGNVLVTARVERKYTLRHNEVCRLNRAL
                     LGDSILEFSLSPDPNAPKDIVKDGDVIKGVVPPDPIIVLTNLQERLVTAINSVNETSQ
                     ALGDTVRSVNRILTENEQQLKELLSNANQTVTAAQQTVTNANEIIGDPELKGRLKEAL
                     SRVPQMVDEIRATLDETNQTIRTARANLENLRGVTEPLAQNGPAIVQRLDNSLTNLER
                     VSAQLAEFSEALNREDTTLGKLVRDPTLYQKLDRTMTNIQSLTQDLRPVISDLRVFSD
                     KIARHPEMLGVRGALERQAGTKGLPNLPSFTNQGQVETYPIEVPASNQRFSVLPGTLR
                     "
     gene            complement(2268..3158)
                     /locus_tag="PKI05_06305"
     CDS             complement(2268..3158)
                     /locus_tag="PKI05_06305"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOA51850.1"
                     /translation="MDPVEREEKLRTVLVALDRKEFLRALSLADQWVTESPSDPVAYS
                     MRAYTLLNLQRSRDALEDARRARELSPDDVTVLSLFAQAAAAEHRLGWAQEAWEKAVA
                     QSGGSPQILREYARFMVHYRGPRLALEAAQRAQQADPKNSVGWSLLAAAQLRMHQYQE
                     AENSLRQALALDPNNVQAQSLMAKLLLLKGKQDQALALSQLLEDDAEARPIAEWIRRE
                     IKKRDLERILVERALDMSAHRPDPGVFLRFRLMWWIVAYIGALALALFLDSLIEGQVF
                     FIFAAVFFVVHVLIWLLLFH"
     gene            complement(3165..>3862)
                     /locus_tag="PKI05_06310"
     CDS             complement(3165..>3862)
                     /locus_tag="PKI05_06310"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HOA51851.1"
                     /translation="LDFFSPRQLYRYTIVKVYESDGGGHRFVSIGFPGFVGCLTGMND
                     AGLCLAVHEVHFTNDGSPICSPHGIPYAMMTRQIMEHCSNVEEAIAHLRKSPRTTLLN
                     IALCDPKRAVVAEITPKTVAVREAERGLLACTNHFCTPELKVFVISRRYGELLEAYNI
                     PKIDVATVQKKLDDVNLGFLSIQSVVFEPEDLVMHLSAGFPPVTKRPYHRLEMRTFLQ
                     SEAKASSPTISSR"
BASE COUNT          918 a         1020 c         1118 g          806 t
ORIGIN      
        1 attgtcaaac gtcctcaagc ttcgtgtacc gggagacctt tcggaggaac gaaatgcggt
       61 attggcaggg gcgttatggc ttaaccccca ttgattgtgt ttcatgatcg ctcaggcaac
      121 gacggaagga acgattagta gcccatcatc taaggacccg gtgttgctcg aggtcgagca
      181 actgaccgtt gccttcggac ggcaggtggt cctgcgcggg ataaatgtac aaatccgtcg
      241 cggggaaacc gtggccatcc tgggagaaag cggctgcgga aagacggttt ttctcaagac
      301 actggtggca ctgcagcggc cgacgaaggg gcgtgtccta tttgatgggc gagacctgtt
      361 tcaattacgg ccacatgagt tggccgaaca gaggattcgc atggggtttg tgtttcaaca
      421 agcggccttg tttgacagca tgacgattgg tgagaacata gcctttccgt tgcgtcagca
      481 cacccgattg acagaggacg agatttacga ggtggtcaag gcccgcttgc tcgaagtggg
      541 actccccgaa aacgtgctgc ggaagaagcc gggagaaatt tccggcggca tgcggaaacg
      601 ggtggggttg gctcgggcac tcgcgctgga gccggagctt atgctatacg acgagccaac
      661 tactggcctc gaccctatca tgagcgatgt gatcaatgaa ctcattcttc ggacgcgacg
      721 tcagcacccc gttaccagcg tgatcgtaac ccacgacatg aggacggcca cgaaggttgc
      781 cgatcgaatc atcatgcttt atcctctgac gcgacttaaa ccgggagaat ctcaaatact
      841 ctacgatggt ccggcgagcg aaattgagtc ggcggcagat gaccgtgtca ttcagtttgt
      901 gcggggcgaa gcgagggaac ggctgatgga aatggcccgc gaggaatcgg aaaacgcggt
      961 tgaaatcgga ggggccgccc atggatgagc gtttgatgca atttcgcatc ggcgtgatgg
     1021 tccttgccag cattctgatt gcggccatcc tcattgtcac gttcggggag tggcgctggc
     1081 catttcccgc ggaatacacg gtttatattc gcttcccaga ggcccctggt gtggccgagc
     1141 gatccccgtt gcgaaaaagc ggcatagtaa tcggctacgt gagaagcgtc gagctaacac
     1201 cggaaggaaa tgtcctggtg accgcccgcg tggaacgaaa atacacgttg cggcacaacg
     1261 aggtgtgtcg tctgaatcga gccctcctgg gggattccat tctggagttt tccctctcgc
     1321 ccgatccaaa cgctcctaaa gacatcgtca aggacggcga tgtgataaag ggggttgttc
     1381 ccccggaccc catcatcgtg ctgaccaatc tccaggagcg cctggtgacg gcaatcaatt
     1441 ccgtgaacga gacatcgcaa gccctaggcg ataccgttcg atcggtcaac cgaatcctca
     1501 cggagaacga acagcagctt aaagaactgc taagtaatgc caatcagacg gtgacagcgg
     1561 ctcaacagac ggtgaccaac gccaatgaaa tcatcggcga tcccgaattg aaaggtcgct
     1621 taaaggaggc cctgagccga gttccacaaa tggtcgatga gattcgcgcc accttagacg
     1681 aaacgaatca gaccattcga acggcacgag cgaaccttga gaatctgcgt ggcgttacag
     1741 aaccgcttgc ccaaaatggt cccgccattg tgcaacgcct tgataacagc ctgacgaacc
     1801 tggagcgcgt cagtgcccaa ttggccgagt tcagcgaggc cctcaatcgg gaggatacga
     1861 ctttgggtaa gcttgtccgg gaccccacgc tttatcaaaa gcttgatcgg actatgacga
     1921 acatccaatc gttaacgcaa gaccttcgac cggtaatcag cgacctgcgg gtattcagcg
     1981 ataaaattgc tcgtcatccc gaaatgctgg gcgtccgtgg tgccctggag cgtcaagccg
     2041 gaacaaaagg ccttccgaat cttccgtcct ttaccaatca agggcaggtc gagacatacc
     2101 cgatagaggt tcccgcgagc aaccagcggt tctccgttct tcctgggacg ttacgctgac
     2161 cgtgtggact ttcctggttg gtgatttcta ggtggccatt ccgcgtgcaa aggggccctt
     2221 cgtctggttg tggcgagcta tcggccttgg cagggagggg aattgcttca gtgaaagagg
     2281 agcagccaga tcagcacgtg gacgacgaaa aacaccgccg caaaaatgaa gaagacttgg
     2341 ccctcgatca aagagtcgag gaataatgct agggccaagg cacctatgta agcaacgatc
     2401 caccacatca ggcgaaaccg aagaaaaacg ccagggtcgg gacggtgagc gctcatgtct
     2461 agggcacgtt cgacaagaat ccgctccaag tcacgcttct taatttcacg gcgaatccac
     2521 tcggcgatgg ggcgggcctc ggcatcgtcc tcgagaagtt gactcaaggc cagagcctga
     2581 tcctgcttcc ccttgagcag caggagctta gccatgagcg attgggcctg gacattgttc
     2641 gggtctaaag ccagggcctg acgtagggag ttttcagcct cctgatactg atgcatccgt
     2701 aattgtgccg ctgccagaag actccaccct accgagtttt ttggatcggc ttgttgcgca
     2761 cgttgagctg cctctaaggc tagccggggg ccgcgataat ggaccatgaa acgggcatat
     2821 tcgcgaagga tttgcgggga accaccactt tgggcgacgg ccttttccca tgcttcttgg
     2881 gcccaaccca ggcggtgttc ggccgcggcg gcttgggcaa aaaggcttag gaccgtgaca
     2941 tcgtcgggcg ataattcccg agcacggcgc gcatcctcaa gggcatcgcg ggaacgctgg
     3001 aggttaagta acgtgtaggc ccgcatactg taggcaacag gatcggacgg cgattctgtc
     3061 acccattggt cggcgaggct gagcgcgcga agaaattcct tgcgatcaag tgcgacaagc
     3121 accgtccgca gtttctcttc ccgctcaacc gggtccatag cccattacct tgagctaata
     3181 gtcggagatg aggccttggc ctctgactga aggaacgttc tcatctccaa tcggtgatag
     3241 ggtcgcttcg tcacaggagg aaaccccgct gataggtgca tcacaaggtc ttctggctcg
     3301 aagacgacgg actgaattga caagaagccc aagttgacat cgtccaattt cttctgaact
     3361 gtagcgacat caattttggg aatgttatat gcctcaagca attctccgta gcgtcgcgaa
     3421 atcacaaaca ctttaagttc aggtgtgcaa aagtgattgg tgcacgctag caatccccgc
     3481 tcggcctcgc ggactgcgac ggtttttggc gtgatttcgg caacgacggc tcgcttaggg
     3541 tcgcaaaggg cgatgttcaa aagggttgta cgagggcttt tcctaagatg ggcgatggcc
     3601 tcttcaacgt tggaacaatg ctccatgatt tgccgcgtca tcatggcgta gggtattcca
     3661 tggggggaac atatcggtga accgtcgtta gtaaagtgta cttcatgcac cgccagacac
     3721 agaccagcgt cattcatgcc ggttaaacac ccaacaaaac cgggaaagcc aatcgagacg
     3781 aaacgatgcc ccccgccgtc ggactcatag accttcacaa tcgtgtaccg gtaaagctgg
     3841 cgtggcgaaa agaaatcgag gt
//
LOCUS       DAOPTR010000268         3857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_18915_length_3857_cov_3.340347, whole genome shotgun sequence.
ACCESSION   DAOPTR010000268 DAOPTR010000000
VERSION     DAOPTR010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18915_length_3857_cov_3.340347"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PKI05_06315"
     CDS             complement(<1..319)
                     /locus_tag="PKI05_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51852.1"
                     /translation="MMDYLSIAAFMLIVGFGGDPPKGSPLVPPAAQSTDFDTAYRCDY
                     SESFDQNYDNWPDYWIRHTGPRYPLYVKAEIVGEPSPEAARCLRVFLDGGSFAATSPK
                     FPVS"
     gene            519..1301
                     /locus_tag="PKI05_06320"
     CDS             519..1301
                     /locus_tag="PKI05_06320"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOA51853.1"
                     /translation="MLRIRLAADLGPIDLPFQQALDHIREINLEAVELDLLKLMPVED
                     FTRTAVREIRKNLADRNLRLAAVRFRTLRGFDDPLGLEQRIAQAKKSMELTYQLGGNL
                     LLIRLGCLPKDRSSTDWQVLEEVVKEFCRFGERAGAIVVAEAERSPPEELAALLNGVP
                     EGGFGVDINPGELAVHGFDPVSAIETLGSRVVTFHATDATMRPGGHGYELAPLGSGGI
                     DYPPILSALEATGFSGFLILRPCGERDPLSEVRRAKEFIYSL"
     gene            1375..2655
                     /locus_tag="PKI05_06325"
     CDS             1375..2655
                     /locus_tag="PKI05_06325"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOA51854.1"
                     /translation="MAKLDAHDACSAPIRQAGSQGRCHSSQRIGLTFSLYARNAVFFV
                     ILMLMIDTWLVSGIVYPIRVAGPSMMPSLDGPRFEIACPQCGYPLCWCAATQSFANCL
                     ERARCPNCGTRLARLMTSGNQQPQPNVRAGNPVVILKGLWRAWKRWDTVAFYNAGEHG
                     GLTVKRIVGLPGERTEIRDGVLWADGRLQPRPWEVQRSMCVPVNLPRNREDSVIHLDA
                     ASSLSQWEVEATHNTVTTYCHFHPALDQYEKPTRNLILRFSLERFSLGTRLHIRSRFG
                     SGHVEATLTLPSEFSVVCFDESPDGTSTKREQISGKLPPLIGEGEFAVSLVDQHFLLI
                     VNDRVITQMFYAARPLAKPIQRPFEVIVERGSVAIHNLGLWKVSCYDDEHRWADPLPG
                     YYVLGDDPHVSLDSRHFGIVAPDRVIGRVIKWPF"
     gene            2687..3733
                     /locus_tag="PKI05_06330"
     CDS             2687..3733
                     /locus_tag="PKI05_06330"
                     /inference="COORDINATES: protein motif:HMM:NF016382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HOA51855.1"
                     /translation="MASQMKRTRNSLAGVNLPDLEAALRRLIAAIPAGHVVSCGTLAE
                     VLGDFRAARWIGSWLGDHQHDPGCCCHRVVRAGGRWGPSPLSQEDREKLLLKEGVLLT
                     SSGADPRCLMDAAQLRALCPEVATKSPLLELQALQVRLCREIRLCPLEREPQSVGGVD
                     VSYRGDMGVAAYCRIAWPSGEKLWDTHIALPTRFPYIVGYLAFRELPLLQAVLDKAEQ
                     EGQLAEVIMVDGSGLLHPRGMGIASHLGLLLNRPTIGVTKTLLCGKCETEDLSPGQSA
                     PIVYRDQVLGAVLRSRTGHAQPVFVCPGHRIDVASAVKIVSPLFGAHRLPEPIYWADR
                     ISRSLARRFIPQNR"
BASE COUNT          765 a         1049 c         1140 g          903 t
ORIGIN      
        1 aagaaacagg aaatttgggt gatgttgcgg caaaggaacc cccgtcgaga aacaccctta
       61 gacaacgcgc cgcctcgggc gaaggctctc caacgatctc ggccttgacg tacagcggat
      121 atctcggccc agtatgacgt atccagtaat cgggccagtt gtcgtagttt tgatcaaaac
      181 tctctgaata gtcgcaacgg taagccgtat caaaatcggt cgattgcgcc gctgggggca
      241 ccaagggaga acccttgggt ggatcccctc caaaccctac aatgagcatg aaagccgcaa
      301 tcgacaggta atccatcata ttgtgtgtcc gtccgggagc gcggccccgc ggttttcgag
      361 cgttgatcac tctccacacc gtggtgtgtg aatccttacg tggggtgcgt tgccacgttg
      421 ggcaaatttg tatcatcgga ctttgctgcg ggtggtctaa agttctcctg gaagcgcttc
      481 ggcttaagca ttgaaaaacg tttaggagga tcaaaacggt gctgcgaatt cgacttgccg
      541 cagacttggg ccccattgac ctaccatttc agcaagctct tgaccacatc cgcgagatca
      601 atttggaggc ggtggaactc gatctattaa agcttatgcc agtggaagat tttaccagaa
      661 cggccgtccg tgaaatccga aaaaacttag ccgaccggaa tcttcgactc gcggcagtac
      721 gattccgaac gttgcgcggc tttgatgatc ccctggggtt ggaacagcgc atcgcgcagg
      781 ccaagaagtc tatggaactg acgtaccaat tgggggggaa tctcctcctg attcggttgg
      841 gatgtctgcc gaaggatcgg tcttctaccg attggcaagt gctggaggaa gtcgtcaagg
      901 agttttgccg gtttggtgaa cgagcgggag cgatcgttgt ggcagaagcc gagcgctcgc
      961 cgccggagga gttggccgcc ctccttaacg gggttccaga ggggggcttc ggggtagata
     1021 tcaatcccgg agagctggca gttcatggtt ttgacccggt ttctgcgatc gagactttgg
     1081 gctcacgggt ggtcacattc cacgctacag atgccacgat gcgaccgggg ggacatggct
     1141 acgagttagc acctttgggg agtggtggca ttgattatcc gcctatccta agtgccttgg
     1201 aggctacagg tttttcgggg ttcctcattc tcagaccatg cggagagcgg gatcccctca
     1261 gcgaggtacg gcgggccaag gagtttatct acagcctttg agtgtaaccg cccatttctt
     1321 tagttctgcc tctttttgcc aagaacgtga ggtaagcgtg tgacggcgcc gccgatggca
     1381 aagttggatg ctcacgacgc ttgttccgcc ccgatccgac aggccggaag ccagggccgt
     1441 tgccattcgt ctcagcggat tggacttacc ttctcgctgt atgcgcggaa tgctgtgttt
     1501 tttgtgattc tcatgctcat gattgacacc tggctggtgt cggggattgt ctatcccatt
     1561 cgcgtcgccg gtccctccat gatgccctca ctggatggcc caaggtttga gatagcgtgt
     1621 ccgcaatgcg gctatcccct gtgttggtgt gcggcaaccc agtcattcgc taattgcctc
     1681 gaacgcgccc gatgtcccaa ctgcgggacc cggctagccc gactgatgac cagcggaaac
     1741 cagcagccgc agccgaacgt tcgggcgggc aatcccgtcg tgatcttgaa agggctttgg
     1801 cgagcttgga agcgctggga caccgtggct ttttataacg ctggcgagca cggcggtctt
     1861 accgtgaaaa ggattgtggg gcttcccggg gaaaggactg agattcgcga cggcgttctt
     1921 tgggcggacg gccgcttgca accccgccct tgggaggttc agcggagcat gtgtgtgccg
     1981 gtcaatctcc cccgcaatag ggaggattct gtgattcacc tcgatgcggc atcgtcgttg
     2041 tcccaatggg aggtggaggc aacccacaac acggtaacga catattgcca ttttcatccg
     2101 gccttggatc agtacgaaaa accaacgcgg aaccttatcc ttcgtttttc cctggaaaga
     2161 ttttcactgg gaacacggct tcacatccgc tcgcgcttcg gctccggcca tgtcgaggcc
     2221 acactgactc tgccatccga gttttcggtt gtgtgttttg atgagagccc tgacggtacc
     2281 agcacgaaac gcgaacaaat ctcggggaag ctgccaccgc tgatcggtga aggtgaattt
     2341 gcagtctccc tcgttgacca acattttctc ctgatcgtta atgatcgtgt gattacgcaa
     2401 atgttctatg cggcccgtcc cctcgccaag cccattcaac ggccttttga agtaatagta
     2461 gagcgcggtt cggtcgcaat tcacaatctc ggcctttgga aggtaagctg ttacgatgac
     2521 gaacaccgct gggcggatcc tttgcccggt tattacgtgt tgggcgatga tccccatgtc
     2581 tcgctggata gtcgccattt cgggattgtc gcccccgatc gcgttattgg ccgagtgatc
     2641 aaatggccgt tctaaaggtc gcttacccat atgcggacat tggcccatgg caagccaaat
     2701 gaaacggaca aggaactcgc tggctggcgt taatttgcct gaccttgagg cggccttgcg
     2761 acgtctcatc gccgcgattc ccgccggtca tgtcgtaagt tgcggaacgc ttgcggaggt
     2821 tttgggagat ttccgcgctg cccggtggat tggctcctgg ttgggcgatc atcagcatga
     2881 cccaggttgc tgctgccatc gcgtggtacg agccggaggc cgatggggcc cttccccact
     2941 ttcccaggaa gatcgggaaa agctcctcct gaaggaaggc gttctgttaa catcgtcggg
     3001 agcggatcca aggtgtttga tggatgccgc gcaactacgt gcgttatgtc cagaagtcgc
     3061 aacgaagtca ccgcttttgg agcttcaggc cttgcaagtc aggctctgcc gcgaaattcg
     3121 actttgcccg ctggaacgtg agccgcaatc ggtaggcggt gttgatgtgt cgtatcgagg
     3181 ggacatgggt gtggcggcgt attgtcgcat cgcttggccg agcggcgaga aactctggga
     3241 tacgcacatc gcactgccaa cgcgattccc ttatatcgtg ggctatctcg cctttcgtga
     3301 gctgccactc ctccaggccg ttctggataa ggccgagcaa gaggggcaat tggcggaggt
     3361 catcatggtc gatggcagtg gccttcttca cccaaggggc atgggcatcg ccagtcatct
     3421 cggcctcctc ctcaatcgtc ccaccatcgg cgtcacgaaa acattgctgt gcgggaaatg
     3481 tgaaacggaa gacctatcgc ccggccaaag tgccccgatc gtttacaggg atcaagtcct
     3541 gggggccgtg ctccggagtc ggacgggcca cgcccagccg gtgttcgtct gtcccggtca
     3601 tcgcattgac gtggcgagtg ccgtgaagat cgtaagtccc ttgttcgggg cccaccgcct
     3661 tcccgagccc atctactggg ctgataggat ttcacgaagc ctggcgcggc ggtttattcc
     3721 gcagaatcgt taagacacat aagagtctat cccataatct cttcttaccc gttccgcggg
     3781 agaggggtcg ggggagaggg tgcctcgaat tgctgccggt tttctcaatc gatgccgacg
     3841 ttcgcttgcc ctcaccc
//
LOCUS       DAOPTR010000269         3848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_18973_length_3848_cov_3.096494, whole genome shotgun sequence.
ACCESSION   DAOPTR010000269 DAOPTR010000000
VERSION     DAOPTR010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18973_length_3848_cov_3.096494"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1929)
                     /locus_tag="PKI05_06335"
     CDS             complement(<1..1929)
                     /locus_tag="PKI05_06335"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOA51856.1"
                     /translation="MSALIFHNVRASLLVLLFLAPFAAGADSGPIRLPPIDQDTAGLP
                     GENPFTAQGLEMRIARRGSDEVPSPLPLDVIGPNVRSTRIEQAARQADLLTFKGYELA
                     GRGAFFAARAQFRAALFVLAQALDTEHRTSEHSQMLDAAWVALKEAEDFLPRPGKQVT
                     SASVPGIIARHDSSVLKDHTFGVLTPLEAYQIYLNFAQDLFSRAVAAEITGSMALRGL
                     GKIYAETSAANGLLQGDSEAQAVVFYQAALNVWPENYMAANDLGVLLARGGRWDDAIS
                     VLEYARRIRNDPVIVRNLVKVYRQQGLADRAAKLLAEAGSLSREPEATDNDVVRSVAW
                     VPPEVLDQADRSLAKGPLGESQFSSSNPVTPSGSHPTIPTKSNTGRLGLLPLLTMGPA
                     DSVAGQSGAARGQNGVVNTPTEFVPGSSAGTQEWPISLGPTADPLTTGVTLPNPASPC
                     PRCAVDGSICDCSKWGGWQRARMIAWEKYAQGEYVGPARLAHVPEYRLRVDDELQIVY
                     RITREETSRPYRLNVGDQLRIESATDPTLNRDVLIQPDGTVTLLLLGQVKATGLTVTQ
                     LRDRLEQLYRRYYRAPAITVTPILVNSKLEDLRATVDRRSGEGGQSQNVRITPEGTIA
                     LPGLGSVKAQGLTLSELQA"
     gene            2927..>3848
                     /locus_tag="PKI05_06340"
     CDS             2927..>3848
                     /locus_tag="PKI05_06340"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOA51857.1"
                     /translation="MNHSSEGNYIWKECVEYAAKTDIGLRRSNNQDAHAEDLAGSLEA
                     FRARGHLFIVADGMGAHAAGETASKKAVEAIPLMYRKLKDLPVKDALREAVLEANRQI
                     FECAQANDNFRGMGTTISALVMSSEGAFVAQVGDSRVYRCRKHSIEQLSRDHSLVWEL
                     RSLPEEIRPQYIPKNIITRSLGPQTDVEVDVEGPFPIEADDRYVLCTDGLSGQLSDEE
                     IGQITTCLPPDEATQALVDLANLRGGPDNITVTVVHVKSAPLCRLSQGNGQSVVSRKA
                     HPPRWLLLAFVVSLLVVLMAPAIPTTSGRAW"
BASE COUNT          846 a         1103 c         1152 g          747 t
ORIGIN      
        1 ggcctgaagt tcgctcaacg tcagcccttg ggccttcacc gagcccaaac cggggagcgc
       61 gattgttccc tccggcgtga tacggacatt ttggctttgg ccaccctcac cagaacggcg
      121 atcgacagtc gcccgcaggt cctccagctt actgttaaca aggataggcg tcaccgtaat
      181 ggccggggca cgataatagc ggcgataaag ttgctcgagg cgatctcgca gttgtgttac
      241 cgtaagtccg gtggccttga cctgacccaa aagaaggagc gtcaccgtcc catccggttg
      301 gatgaggacg tcgcggttca atgtgggatc agtggctgac tcgatgcgca gttgatctcc
      361 cacgttcaag cggtacggtc gcgaggtctc ctcccgcgtg atgcgataga cgatttgcag
      421 ttcgtcgtcc acgcggagcc ggtattccgg gacatgggca agccgcgcgg ggcccacgta
      481 ttctccctgg gcgtatttct cccaagcgat catccgtgcc cgctgccagc ctccccactt
      541 ggagcaatca caaatggaac cgtccaccgc gcagcgcgga cacggcgaag cgggattcgg
      601 caaggtaaca cccgtggtca gagggtccgc cgtcgggccg agggagatag gccactcttg
      661 tgttcccgcc gaagaacccg gcacaaactc ggtcggggta ttgacgactc cattctggcc
      721 ccgagctgcg ccggattggc cggcgacaga gtcggcgggg cccatggtca acaatggtag
      781 caaccccaat cggcctgtat tgcttttcgt gggaatggtg gggtgggatc cgctgggcgt
      841 caccgggttt gaagatgaaa actgcgactc gccgagaggc cccttcgcta ggctgcgatc
      901 cgcctgatca agcacctcgg gcggaaccca agccacactc cggacaacgt cgttatcggt
      961 cgcctcgggt tctcgcgaca gagaaccagc ctccgcaagt agcttcgcgg cacggtcggc
     1021 cagtccttgt tgtcgataaa cttttactag attgcgcaca atgacaggat cgttccgaat
     1081 ccgacgggca tactccaata cggaaatggc gtcgtcccat cggccacccc gggccaacag
     1141 gacgccaagg tcgttggcag ccatgtagtt ctccggccag acattgagag ctgcctggta
     1201 aaagaccacc gcttgggcct cggaatcacc ctgaagcaga ccgtttgccg cgctcgtctc
     1261 cgcatagatt ttgcccaacc cgcgaagggc catcgagccg gtgatttccg cggctaccgc
     1321 gcgagaaaac aagtcctgag caaagttcag atagatctga tacgcctcca ggggggtcag
     1381 caccccgaag gtatggtctt taagcacaga ggaatcatgt cgggcaatga tccccggaac
     1441 ggatgcggat gtcacctgct ttcctggccg cgggaggaag tcctcagcct cctttagggc
     1501 aacccaggcg gcatccagca tctgggagtg ctccgacgtc cggtgttcgg tatcgagggc
     1561 ctgggcaagg acgaagaggg cagcgcgaaa ctgggcccga gcggcaaaaa aggcccctcg
     1621 tccggccagc tcgtagccct tgaacgtcaa caaatcggcc tgtcgggcgg cctgctcaat
     1681 ccgagtggag cggacgttcg gtccaattac gtcaagcgga agcggcgagg gtacctcatc
     1741 tgacccgcgg cgggcaatcc gcatctcaag cccttgagcc gtgaaaggat tctcgcccgg
     1801 caagccggca gtatcttggt caatgggggg cagtcgaata ggaccggaat cggcaccggc
     1861 agcaaacggg gctaaaaaaa gcagaaccaa aagacttgcc cgcacgttat gaaagatgag
     1921 agcggacatc tttgaaaccg ctgcctggag tatcatcgtt gagttctttc ttgctactgc
     1981 gcggcgcatc acataaatcg atcacaaccg gctcctgaca atctgttctt acaatcccat
     2041 cgtccgcgag agcgagcatc tccgaccaaa gcgatgagcg cgttttcctc gacagaacga
     2101 tcaaagacct ggatttcgta atacccgaca agctcgacac aaccgggcgc cgagtcgata
     2161 tcaaagctgc ttgtgtctcg gcacgttcat gaaacgcggc agagcttacc aacgtccgac
     2221 aactttagtg ctgcgaaaga gaaatggggc cagcagtttt cttgcctgct gccgccggta
     2281 aaggttggcc tgccgatagc tgcggactaa aatgaggatc agtaagattg ggcaaaataa
     2341 ccaaatcccg gatattgaac aagatacaga aagcgtggcg tccgataatc tcaatgtgtg
     2401 tcggatgttg cgggagacgt tacccgtttg ggctgtgcag taggtctggc tgaacccagc
     2461 caaaggagag tcaaggttac gacggcggtc ctcgatttcc taaaagcgtc gggcggtcgt
     2521 ggcggttcgg gcctagcacg aaggtctttg aggcggcaca actggatcta ggcattcccg
     2581 gcttggacag gggcgcacaa tggcgcatca atcggcagcg ccgcggacac gatggcgcgc
     2641 aaccgggtag attttgggga acatggccag tccgtccaaa cccggcggtg ggcaatgacg
     2701 cggtgttgtg gccgtggcat agatggcggg cccccactgc aagataggag ggtgttttct
     2761 gacacagatt tcaactggcc agtttgcggt ggcaatttaa atgacaatcg gacgcgatgg
     2821 cacggttatc ggcaagcgta cgatccgggg gccaaaaata ttgacaatgc cgcaattcgg
     2881 gtggtcaaat gaatgaacgt tttccatgcc ttgaacggcc aatgtgatga atcattcatc
     2941 tgagggcaat tacatttgga aggaatgcgt cgaatacgca gccaagaccg acatcgggct
     3001 gcggcggtcg aacaaccaag acgcccatgc cgaggacctc gccggtagcc tggaggcatt
     3061 tcgcgctcgt ggacacctgt tcatcgttgc ggacggcatg ggtgctcacg cagcgggaga
     3121 aacggccagc aagaaagcgg tcgaagccat cccgctgatg taccgcaagt tgaaggactt
     3181 gcccgtcaag gacgccctgc gagaggctgt cttagaagcc aatcggcaga tctttgagtg
     3241 tgcccaagcc aacgacaact ttcgcgggat gggcacgacg atcagcgcgc ttgtcatgtc
     3301 ttccgagggt gccttcgtgg cccaagtggg agacagccgt gtctatcgct gccgcaagca
     3361 ctccattgag caactctccc gagaccatag tctcgtctgg gagcttcgca gtctgcccga
     3421 agaaattcga cctcagtata tcccgaaaaa catcatcacg cgttcccttg gcccgcagac
     3481 ggacgttgag gtggatgtgg aaggcccctt tcccatcgag gccgacgacc gttatgtgct
     3541 gtgtaccgat ggcctatccg ggcaattgag cgacgaggag atcggacaga tcactacgtg
     3601 ccttccaccc gacgaggcca cacaagcctt agtggatctg gccaacctcc gcggtgggcc
     3661 ggataacatt acggtcacgg tggttcacgt caagtctgct ccattatgtc ggctaagcca
     3721 aggaaacggg caatcggtgg tctcgaggaa ggcccatccg ccgcgctggc tcctacttgc
     3781 ctttgtcgtg tcgttgctcg tcgtgctcat ggctccggcg atccccacca cgagcggacg
     3841 tgcgtggc
//
LOCUS       DAOPTR010000270         3847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_18980_length_3847_cov_4.277954, whole genome shotgun sequence.
ACCESSION   DAOPTR010000270 DAOPTR010000000
VERSION     DAOPTR010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18980_length_3847_cov_4.277954"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..852)
                     /locus_tag="PKI05_06345"
     CDS             complement(61..852)
                     /locus_tag="PKI05_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA51858.1"
                     /translation="MSDQGCRLSQRERKLVAENVTKSFRHVATTIQVLRGVSFSLLTG
                     EFVAIMGPSGSGKSTLLHVLAGLTPPDSGRVSLDGYEIGELSDREMTALRRRKIGIVF
                     QQFNLIPALTLEENVLLPLLAERVDRSRLEKARQIIRDLGLWERRHHRPDALSGGEQQ
                     RTAIARALAIDPTLILADEPTGNLDSIAGQQFCHLLKTLAKEQSRTILVVTHEPAVAI
                     WTDRVLVLKDGQIVAEFPTESCNSVRSLAARYEESLQMGQLENTL"
     gene            complement(882..1949)
                     /locus_tag="PKI05_06350"
     CDS             complement(882..1949)
                     /locus_tag="PKI05_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007330089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOA51859.1"
                     /translation="MRVITSTELGSKKLGDDYINTARCMERDGFTLVELLVVIAIIGL
                     LIALLLPAVQAAREAARRAQCCNHLRQLGLAMQNYHNAYQMLPNTGFFGPTYPNDYSP
                     LAKLLPFVEQQNLHNLVDFRITLGHVGREDLPECLWSAARTPVSLFLCPSDGEKTIHD
                     LTLPSGRVIPVAGANYAMNQGSGLDGMFNPGFGATDGLCWVGAEIRLDDVLDGTSNTL
                     VFTESLRGPGTSLSASDRPNHQIYRARAVPTLIDVVEQQGLEALYPQVTGWDGTRLMF
                     WLRGCSPTGPVMNGRLLPNSRIPDLVGGSEKVTAARSWHPGGVNACFCDGSVRFIAQT
                     ISATSWHAFWTRAGGEVVSVP"
     gene            2650..3177
                     /locus_tag="PKI05_06355"
     CDS             2650..3177
                     /locus_tag="PKI05_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HOA51860.1"
                     /translation="MPNVLRMRTYAGAATIGSFFVVSLTGIIMFFHINIGWIKMAHEW
                     MSWVFVAAAVCHAVVHWRSLLGYFRNRAALAVIIPIVIIGLLSLSPVHGPASKHPLAQ
                     VMFRLEHASLESVATLTDRDVSEVVRRLKASGITIDTTNQTITEIATRNGRRPTEVLL
                     LLFSEEKPIKAVRHK"
     gene            complement(3381..>3847)
                     /locus_tag="PKI05_06360"
     CDS             complement(3381..>3847)
                     /locus_tag="PKI05_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51861.1"
                     /translation="AKLLEGYQILSELGLPLYITGITIPSAGEQGEEIQAELVRNLYR
                     LWFSVAMMAGITWWNLADGTAVQGENEARGGLMTEDLQPKPAYRELEQLIHQEWETRA
                     NGQTDQEGTYRVRGFHGRYLVRVGKESTWVVRTLTVDQKIPATLEVLWVENK"
BASE COUNT          935 a         1065 c          941 g          906 t
ORIGIN      
        1 ccgcaacacg tccggggtag tgcaacgcgt cggccaaagc aagtcttgca ctgacgggca
       61 tcatagcgtg ttttccagtt gtcccatctg aaggctctct tcatatcgtg ctgccagaga
      121 gcgcacggaa ttgcagcttt ctgtaggaaa ctcggcaacg atttggccgt ctttcagaac
      181 caagacacgg tctgtccaga ttgccacggc tggctcatgc gtcaccacca ggatggtgcg
      241 ggactgttct ttcgcgagcg tcttaagcag atgacagaac tgttgaccgg ctattgaatc
      301 aaggtttccc gttggttcat cggctaggat aagggtcggg tcaatagcca gggctcttgc
      361 gatcgctgtc cgctgttgtt cgccgccact caaagcatcg ggtcgatgat ggcgccgctc
      421 ccaaagcccc aagtcacgaa tgatctgacg cgctttctcc aacctactcc ggtcaacccg
      481 ctccgccaga aggggcaaaa gcacattctc ttccaaagtc agcgcgggaa tcagattaaa
      541 ttgttgaaaa acgatcccaa tcttgcgacg tcgaagggct gtcatttctc gatccgaaag
      601 ctcgccgatt tcgtaaccgt ccaagctaac acgccctgaa tccggtggtg tgagtccagc
      661 aagaacatgc aataacgtgc ttttgcccga accacttggc cccatgatgg ccacaaattc
      721 ccccgtcaaa agcgaaaaag agacaccacg gagaacctga atcgtcgtgg cgacgtgccg
      781 gaagctcttc gttacgttct ccgcaacaag cttgcgttct ctctgactca gacgacaccc
      841 ctgatcactc atttcaattc ctttgccgga aaagccgcat gttagggtac actcaccact
      901 tcaccgccag cacgagtcca aaaggcatgc caagaggttg ctgaaatggt ttgggcgata
      961 aagcgtacgc ttccgtcaca gaaacaggcg ttaaccccac caggatgcca actcctggct
     1021 gctgtcactt tttcggaacc accaaccaga tctgggatgc gggagttggg taacaaccgc
     1081 ccgttcatga cgggaccagt tggagagcat ccgcgcagcc aaaacattag tcgagtccca
     1141 tcccaacccg ttacctgcgg atacaacgct tccaaaccct gctgctcaac cacgtcaatc
     1201 aacgtgggaa cagctctagc tcggtaaatt tgatgattgg gccggtcact ggccgataag
     1261 gatgttcctg gcccgcgcaa tgattcagtg aacacaagag tgttactcgt gccgtcaaga
     1321 acatcgtcca gtcggatttc ggctcccacc caacaaagac catccgtggc accaaagcct
     1381 ggattaaaca taccgtctag gccactgccc tgattcatag catagtttgc cccggccacg
     1441 ggaatgacac gaccactagg caacgttaga tcatgaatcg tcttttcgcc gtcactcgga
     1501 caaaggaaga gagaaaccgg agttcgggcg gcggaccaga ggcattctgg aagatcttcc
     1561 cggcctacat gtcccaatgt aattcggaaa tcgaccaagt tatgaagatt ttgctgctca
     1621 acgaatggaa gaagcttcgc caacggtgaa tagtcatttg ggtatgtggg accaaaaaag
     1681 cctgtattcg gcaacatttg ataggcgtta tgatagtttt gcatcgctag ccccaattgc
     1741 cgtaaatggt tacagcattg ggcacggcga gcagcttcac gagctgcttg aactgccgga
     1801 agaagaagtg ctatcagcaa cccaatgatg gcaatgacta cgagcaattc caccaaggtg
     1861 aagccgtctc gctccatgca ccgggcggtg ttgatataat catcccccag ttttttcgat
     1921 ccgagctcgg tggaagtgat cactctcata cagtgtctcc cgagattccg tccctggccg
     1981 gggtaaaagt ttgtcatacg aatgctcatg caattcggat gccacagctc aaactacgct
     2041 ccaatagcgt tcgaaggctg ttttcatcgc tccgcaaacg aagttcccag aaaaacctca
     2101 tcagtgtctt gttggttcgc gcgacactgt atgttgcgca caacgacata attacccacg
     2161 ccgaaaagaa acaacccacc agatacttga cacctatttc ctgactggca gttttatcgg
     2221 aggagttgct ggacacatca acgtacttcg ccaagacacc agactgagct attgtggagc
     2281 tcctctagtc aataaaggca aacctgctaa ctgcgttggc gactgtcgaa ctcctagaca
     2341 aaatactctc cctgaggagc ggtcccgaaa ccaacgagga ctcttgggtg ttcatacttg
     2401 tgggatcggt ctcagacaca cattggggaa cgccaaacta ggcgggctgc tgggcatcat
     2461 gctatgccga atggattctt cagacgtgtg gcacgagact tgcattagta gtattgatcc
     2521 gactccatcg gttgacccgt tgcccagcat aacggtgcag cgattgcagg caagtcatgt
     2581 aaagtcaatt tcttgaatac gttggataaa ggaattgccg gatttttttg gacaaaggaa
     2641 gttgatacca tgcctaacgt cttacggatg cgaacgtatg ctggtgcagc aaccattggc
     2701 tcgttttttg tcgtatccct gacaggcatc atcatgttct tccacatcaa tatcggatgg
     2761 atcaagatgg cccacgagtg gatgagctgg gtcttcgtgg ccgcagccgt atgtcacgcc
     2821 gtggttcatt ggagatcgct tctagggtac tttcgcaacc gggcggcact cgccgtgatt
     2881 attcctatcg tcatcatagg tttgctttct ctgtctcccg tgcacggccc agcctcgaaa
     2941 cacccgctgg ctcaagtgat gttccgtttg gagcacgcat ccctcgagtc agtggcgacg
     3001 ctgacggatc gggatgtatc cgaggtagta cgtcgcttga aagcgtcggg aattacgatc
     3061 gacaccacca accagacaat tacggaaatt gccacaagaa atggcagacg gccaacggaa
     3121 gtcttgcttc tactattttc agaagaaaaa cccattaaag ccgtacggca taaatgatac
     3181 cggtcccgct atatggcgag ttcgaagaat tgtctcttta tatctttatc acacggatga
     3241 ggccacaata gaggaaaacc gaggcgtcta attttcctca aaagagtttt catatctcgc
     3301 aagccataat aatccgtcgc gtttactcct ggtcgcgtct tgcgtggctc cgttgtccat
     3361 tcgtttgaca aaccggccag ttacttgttt tccacccata ggacttccag tgttgcgggt
     3421 atcttttgat cgactgtcaa cgtccgcacg acccaagtgc tttccttccc cacacgaacc
     3481 aagtaccgcc catggaaccc ccgcacacga taggtgccct cttggtcggt ttggccgttt
     3541 gcccgtgttt cccactcctg gtgaataagc tgttcaagct cccggtaggc cggttttggc
     3601 tgcaagtcct ccgtcatcaa gccgccgcga gcttcatttt ctccctgcac ggcggtgcca
     3661 tcggcgagat tccaccaggt gatcccggcc atcattgcca cgctaaacca tagccgataa
     3721 agatttcgca ccagttccgc ttggatctcc tcgccctgtt caccagcgga cgggatcgtg
     3781 atccccgtaa tataaagcgg caaaccaagt tcactaagga tttggtaacc ttctaggagt
     3841 ttggcag
//
LOCUS       DAOPTR010000271         3844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19004_length_3844_cov_4.040644, whole genome shotgun sequence.
ACCESSION   DAOPTR010000271 DAOPTR010000000
VERSION     DAOPTR010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19004_length_3844_cov_4.040644"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /gene="gatB"
                     /locus_tag="PKI05_06365"
     CDS             <1..1083
                     /gene="gatB"
                     /locus_tag="PKI05_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOA51862.1"
                     /translation="GKSMHDEKAGRADSRIDLNRAGTPLLEIVTQPDIRSPAEAKAFL
                     TELKLLLNYLEVSDCNMQEGSLRVDANINLHIPTEDGQYVATPIVEVKNMNSFRAVER
                     ALTYEAQRQYEAFLETGHRLGDVPKQTRGWDEDAQLTRPQRSKEESSDYRYFPEPDLV
                     PVTVTDAELEALRQQLGELPADIRRRLQETYHLNDYDSDVIVNQGRAFVDYFCQLADT
                     CGDGKMAANWAQQDVLRTLNERDWTITQFPISAAELARLLCRVKSGELDTAKARMVFG
                     HMVDHQATVDEAIQSLGIVQVDETELRQLCAELLASNPKVVAEVKSGKDKAIGSLVGQ
                     AKKRNPHVNPARVKEICLELIRQETT"
     gene            1181..1960
                     /locus_tag="PKI05_06370"
     CDS             1181..1960
                     /locus_tag="PKI05_06370"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOA51863.1"
                     /translation="MTQIVTLITDFGWESVYVAQMKAVLLSINRRLTIVDVTHEIPPQ
                     DVQAGALVLRHVVPSFPPGSCHVAVVDPGVGTQRPCLCADMGGRYVVAPDNGLITYLA
                     EVLPVNVMVRLENPAYRLPEVSATFHGRDVMAPAAAWLSLGVTLEQLGPVHHAPVLLP
                     EGWKPLVTERQIRGKVVLVDRFGNLITNIEKTHLSPDRTVTVSIGEYVIPGLVRTYGH
                     RTSGEVVALINSQGYVEVAVVNGSAAEVTGLGVGTPVVITW"
     gene            1967..3328
                     /gene="bioA"
                     /locus_tag="PKI05_06375"
     CDS             1967..3328
                     /gene="bioA"
                     /locus_tag="PKI05_06375"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HOA51864.1"
                     /translation="MLDEREKQQLRQWDRTIYWHAFTQMAEYEPFIIERAEGCRLIDI
                     DGREYIDGVASLWCNVHGHRHPKLDAAIREQLDRVAHVTSLGCSNPTTIRLAKRLTEI
                     APPGLRHVFFADSGAGAVEVALKVAFQYWRQIPRPQPQKTVFLALGDAYHGDTLGAVS
                     VGGVDRFNAVFQPLMFPTIRLPCPETYRTPEGVPPSELTEYYLNQVEQVLRREHERIV
                     AMIIEPLVQCAAGIVMHPLGYLRGVRELTRKYNVLLIADEVAVGFGRTGRMFACEHEG
                     VTPDLLCLGKGITGGYLPVAATLVTDEVYNAFLGDYASLRTFFHGHTYGGNPLGAAVA
                     LANLDVFEEERTLDNLPPKIERLRAHLERIANRPHVGHVRQCGLIAGIELVQDKATKK
                     PYPWTERRGWQVCLAVREEGIFLRPLGDVIVIFPPLAISLEEIDQIGFAVERAIIRVT
                     EQD"
     gene            complement(3495..>3844)
                     /locus_tag="PKI05_06380"
     CDS             complement(3495..>3844)
                     /locus_tag="PKI05_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51865.1"
                     /translation="RDPVPQRMHIPPMEDQWSCSLFASQDPVAIDSVAFDFLYAEGTP
                     FPRKGGVDDYLHEAALADQPPSGTFYDPNHAEPTQRLASLGVHEHWNNVKEKKYSRNL
                     GASEGIELLPVTL"
BASE COUNT          807 a         1050 c         1162 g          825 t
ORIGIN      
        1 ggaaagagta tgcacgacga gaaggccggg cgggctgata gccggatcga cctcaatcgc
       61 gccgggacac ctcttttgga aatcgtcacc cagcccgaca tccgttcccc tgccgaagcc
      121 aaggcgttct tgaccgagtt aaagctcctt cttaactatc tggaggtctc cgactgcaac
      181 atgcaggagg ggagcctccg cgtggatgcc aacatcaatc tgcatattcc cacggaggat
      241 gggcagtatg tggccacgcc gattgtggaa gtcaagaaca tgaacagctt tcgggctgtg
      301 gaacgggcat tgacctacga ggctcagcgg caatatgaag catttcttga gaccgggcac
      361 cgactgggag atgttcccaa gcaaacacgg ggttgggatg aggatgctca attgacccgg
      421 ccccagcgat ccaaggagga gtccagtgat taccgctatt tcccggaacc tgacctggtc
      481 cccgtcacgg tgaccgatgc tgaacttgag gccctgcggc agcagttggg cgagcttccg
      541 gccgacatcc gccgacgatt acaggaaacc tatcatctga acgactacga tagtgatgtc
      601 attgtgaatc aagggcgggc atttgtcgat tacttttgcc agttggcgga tacctgtggg
      661 gatggcaaga tggccgccaa ttgggcccag caggatgtct tgcggacgct gaacgagcgg
      721 gactggacga tcacccaatt tcccatttcc gcggcggaac tggcaagact tctttgtcgt
      781 gtcaaaagcg gtgaactcga taccgctaag gcgcgcatgg tcttcggcca tatggtggac
      841 caccaagcca ccgtggatga ggctatccag tcgctgggga ttgtccaggt tgacgaaaca
      901 gagttgcgcc agttgtgcgc agagctcctc gcttccaatc ccaaagtcgt ggcagaagta
      961 aagtccggca aagacaaggc cattggcagt ctcgtagggc aagctaaaaa acgtaacccg
     1021 catgtcaatc ctgcaagggt gaaagaaatc tgtttggagt tgatccggca ggaaacgacg
     1081 tgaaccgccg tagccctgga cgaccaccac ggtgcttccc ctcggttaag ctgatcggct
     1141 ttgtgtggac ctccactcgc atgggcagca tgggttgcat atgacgcaga ttgttaccct
     1201 catcaccgat ttcgggtggg aaagtgttta cgtcgcccaa atgaaggcag tattgctttc
     1261 catcaatcgg cgtctcacca ttgtggacgt cacgcacgag atcccacccc aggacgttca
     1321 ggccggcgcc ttggttctac ggcacgttgt gcctagcttt cctcccggga gttgccatgt
     1381 ggcagtggtc gatcccggtg tggggacaca gcggccatgc ctttgtgccg atatgggagg
     1441 acgctatgtt gtcgctccgg ataatgggct catcacctat cttgccgaag tgctgcctgt
     1501 taacgtgatg gtccgcctag aaaacccggc ttatcgcctg cccgaggtaa gtgccacatt
     1561 tcatggcagg gacgtaatgg ccccggccgc cgcgtggctg agccttggcg tgacgctgga
     1621 acagttggga cccgtccatc acgcccctgt gcttttgccc gagggatgga agccactcgt
     1681 gacggagcgg cagattcggg ggaaggtcgt tttggtcgat cgatttggaa acctcatcac
     1741 caatatcgaa aagacacatc tgtcgccgga ccgaactgtg accgtgagca ttggggagta
     1801 tgttattccg gggttggttc ggacttacgg ccatcgcaca tcaggggaag tagtggccct
     1861 gattaactct caaggctatg tggaggttgc ggtcgtcaac ggcagcgcgg ccgaggtaac
     1921 gggtttaggt gtgggaactc ctgtggtgat tacttggtaa gggacgatgt tggacgaacg
     1981 agaaaaacag caactccgcc agtgggatcg gaccatctat tggcacgcct tcacacagat
     2041 ggcggaatac gagcctttta tcatcgaacg cgcggaaggt tgccgtctca ttgatatcga
     2101 cggtcgggaa tacatcgatg gtgtggccag tctgtggtgt aacgtccatg gccatcgtca
     2161 tccaaaactg gacgccgcaa tccgcgagca acttgatcgt gtggcccacg tcacatcatt
     2221 gggatgttcc aacccgacga cgatccgtct ggccaaacgt ctgacggaga ttgcgccgcc
     2281 tggtttgcgt cacgtgtttt ttgcggacag cggtgctggg gcggtggaag tggccctcaa
     2341 ggttgccttt cagtattggc ggcaaattcc tcgaccgcag ccccaaaaga ccgtgttttt
     2401 ggctctcggc gatgcctacc acggggacac gcttggggcg gtgagtgtag gtggtgtgga
     2461 tcgtttcaat gccgtctttc agccgctcat gtttcccaca atccgccttc cctgccccga
     2521 aacctaccgg acacctgaag gcgttccccc gagcgagcta accgagtatt acctcaacca
     2581 agtcgagcag gtcctgcggc gggaacatga gcggatcgtg gcgatgatca ttgaaccact
     2641 ggtccagtgt gcggcgggaa tcgtgatgca tccgctgggt tatctgcgag gagtccgcga
     2701 gctaacacgc aaatacaatg ttctcttgat cgctgatgaa gtagcggtgg gttttggtcg
     2761 cactggccgg atgtttgcct gcgagcacga aggtgtcacg cccgacttgt tatgccttgg
     2821 aaaaggcatc accggagggt atctgccggt cgctgcgacg ctggtcacgg acgaggtgta
     2881 taacgccttt ctcggtgact acgcctcatt acgaacgttt tttcacgggc acacctacgg
     2941 gggcaatccc cttggcgcgg cggtggccct ggcaaacttg gacgtcttcg aggaagaaag
     3001 gacactggat aaccttccgc cgaagattga gcgactaagg gcacacctcg agcggatcgc
     3061 caatcgcccc cacgttggtc atgtccgcca atgcggactg attgccggaa tcgagttagt
     3121 ccaggacaaa gcaactaaaa aaccctaccc gtggacggaa cgccgtggct ggcaggtgtg
     3181 cctggccgtc cgcgaggagg gtatattcct gcggccgctt ggtgatgtga ttgtgatctt
     3241 tccccccctg gctattagcc ttgaggagat cgatcagatt ggctttgccg tggaacgggc
     3301 cattattcgc gtcacagaac aggattaaga ccccctcacc ctctgggaga gggttggggt
     3361 gagggcaacc ggctgtcgga gtcgatcaag aaaaccggca ttttcgagcc accctctccc
     3421 gccgaacgcg tgaggggaga ttatgggata ggctctcatc gagccgccgg cgccctcggc
     3481 ctcggttcca agcgtcaaag tgtgacgggc aaaagctcga tcccttcgct ggcccccaaa
     3541 ttgcgcgagt actttttctc cttgacgttg ttccagtgtt cgtgaacgcc gaggctggcg
     3601 agacgctgcg tcggttcagc atgattgggg tcgtaaaatg tccccgaggg cggctgatcg
     3661 gccaaggccg cctcgtggag gtaatcgtcc acgccgcctt tccgcgggaa gggcgtgcct
     3721 tcggcataga gaaagtcaaa cgccacggaa tcgatggcca ccggatcttg cgaggcaaac
     3781 aagctgcacg accactgatc ctccatcggt gggatatgca tccgttgcgg caccggatcg
     3841 cgcg
//
LOCUS       DAOPTR010000272         3832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19086_length_3832_cov_3.803018, whole genome shotgun sequence.
ACCESSION   DAOPTR010000272 DAOPTR010000000
VERSION     DAOPTR010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19086_length_3832_cov_3.803018"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PKI05_06385"
     CDS             <1..226
                     /locus_tag="PKI05_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910851.1"
                     /note="catalyzes the formation of oxalozcetate and
                     L-glutamate from L-aspartate and 2-oxoglutarate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOA51866.1"
                     /translation="YVRCPREVAGRRFETAEELAHFLIEEHSIVTVPWDDAGPHLRFS
                     VTYLAASEEEEDALMAETERRLMSLNPKFD"
     gene            311..1126
                     /locus_tag="PKI05_06390"
     CDS             311..1126
                     /locus_tag="PKI05_06390"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOA51867.1"
                     /translation="MVGEIRYLVFDVESVADPNLVARLRYPNERLSPEEAVERYRQEL
                     IEKYETDFIPYTYQIPVSIVVAKVNSQLRLVDLVALDEPLFRPHIMTERFWRGWEAYK
                     QPTLVTFNGRAFDIPLLELAAFRYGISIPGWFQVNSKSFEQPRNRYNTQYHLDLCELL
                     TNYGMTRFTGGLNLAANLLGKPGKMDVAGYMVQDLYREGRLAEINDYCRCDVLDTYFI
                     FLRTRVLVGQISLEEEHRIVAEAKQWLEQRAATDRACRIYLDHWGDWPNPWVE"
     gene            complement(1180..1839)
                     /locus_tag="PKI05_06395"
     CDS             complement(1180..1839)
                     /locus_tag="PKI05_06395"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOA51868.1"
                     /translation="MLPRVLETEVMDTLEEALEYDSMDHSEVNQRFVDDFVRAGGHPG
                     VILDLGCGTALIPILLAEKLPSSRIIAVDYSQNMLLIGRQHLIDKNLSSAVALLRADA
                     KRLPFAEAMFSQVVSNSLIHHLADPKIAFSEAYRVTRPGGLLFFRDLARPTDRATLNR
                     LVQLYAGNANAHQQQLLADSLAAALTIEEVSELVADFALLEHSLTMTSDRHWTWIARK
                     R"
     gene            complement(2033..3316)
                     /locus_tag="PKI05_06400"
     CDS             complement(2033..3316)
                     /locus_tag="PKI05_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51869.1"
                     /translation="MNLKWSKQLRWLTFGCCLCAFLGYLPIAQGNEEGKGSAMTYGQV
                     RKYLAQHTKILELVNEEGGRVLVCPEWQGRVMTSSCDGLQGLSFGFINKEFIDAGQPN
                     PHFNNYGGEDRFWLSPEGGPFSLWFKHGVDQVLDNWFTPPALNEGAWTVISKPRDPFF
                     RMFRKMELVNTAGTQFRLEVTRDVRLLSENDLQRLFGGDVATAVRSRKVKSVGYETVN
                     RVVNAGEPFDKAKGLVSIWILGMFNAGPQTVIIVPYKQGDEATLGPVVKSDYFGPIPP
                     ERLKILPEAILFLGDGNYRSKIGTSQKRAKNVAGSMDFQNNVLTLVQFTMPEDPSQFL
                     YMNNMWGIPQAEPYVGDVANSYNDGPAGPDKPGLGPFYEIESLSPALELKTGEKLEHA
                     HRTLHIQGDYETLRSIAQKVLGVDLNVVRQTMFGQ"
BASE COUNT          855 a         1047 c         1015 g          915 t
ORIGIN      
        1 gtacgtacga tgtccccgag aggtggcagg ccggcggttc gagacggccg aagagcttgc
       61 ccattttttg atcgaggagc actcgatcgt taccgtacca tgggatgacg ccgggccgca
      121 cttgagattc tcggttacct atctagcggc aagtgaggag gaggaagatg cgctgatggc
      181 cgagaccgaa cgacggctca tgtcacttaa tccaaagttt gactgaaaag gctttcgcca
      241 aggcgagacc ccaatatttg ggtgctctgc ctggctcgag tgcgccatct tcattgcgag
      301 aggcggtgtg atggtgggtg aaattcggta cctcgtgttt gatgtcgaga gtgtggccga
      361 cccgaatctg gtggcgaggc ttcgttatcc aaacgaaagg ctatcaccag aggaggcggt
      421 ggaacgatat cgccaagagc ttatcgagaa atacgagact gattttattc cctacacgta
      481 ccaaatcccg gtttccattg ttgttgccaa ggtgaatagc cagttgcgtc tagtcgattt
      541 ggtggcgctc gacgaacccc tctttcgacc tcacattatg acagaacgct tttggcgagg
      601 ttgggaggcg tacaagcaac ccacgttggt gacgttcaat ggtcgggctt ttgatatccc
      661 tctcctcgaa ctggcagcct ttcgttacgg catcagcatc ccggggtggt ttcaggtcaa
      721 ttcgaaatcg tttgaacagc cccgtaaccg gtacaacacg cagtaccatc ttgacctgtg
      781 cgagctgctt acgaattacg gcatgacgcg atttacgggc ggattgaacc tggccgccaa
      841 cctcctcggc aagccgggta agatggatgt agcaggctac atggtccagg acctttaccg
      901 cgaaggacgg ttggccgaga ttaacgacta ctgccgttgc gacgtgctcg atacctattt
      961 cattttcttg cgaactcggg ttcttgtcgg gcaaatcagt ctggaagagg agcaccgcat
     1021 tgtggccgag gccaaacagt ggcttgagca gcgcgccgct accgaccgcg cctgccgtat
     1081 ctacctcgat cattggggcg attggcccaa tccctgggtg gagtgaaaaa cggcctcgta
     1141 aggcacatgc tagggaacct ctagtcaccc cgcttgagat tatctttttc gggcaatcca
     1201 cgtccagtgc cgatcactgg tcatggtaag gctatgctcc aagagcgcga aatccgcaac
     1261 caactccgac acttcctcaa tggtcaatgc cgccgcgagc gaatcggcca atagttgctg
     1321 ctgatgcgcg ttcgcattgc ctgcataaag ttgaaccaaa cggttcagag tggccctgtc
     1381 ggtgggccgt gctaagtcgc ggaagaacaa caaaccacca ggccttgtga ctcggtaggc
     1441 ttcgctgaaa gcaattttgg gatccgcaag atggtggatg aggctatttg acacaacttg
     1501 ggaaaacatt gcctcggcga agggaaggcg tttggcatcg gcacggagta gcgccacggc
     1561 ggatgaaaga ttcttgtcaa taagatgctg tcgtccgata agcagcatat tttgggaata
     1621 gtccacggca ataatccggg acgagggaag cttctcggcc agcaatatgg gaatcagcgc
     1681 cgtaccacag cctaagtcga ggatcacacc gggatggccg cccgctcgga caaaatcatc
     1741 aacaaaccgc tggttgacct cgctgtgatc catgctgtcg tactccagcg cctcttccag
     1801 cgtgtccatc acttccgttt ctaaaacacg gggcaacatg gcagatgcgt ggtgagttct
     1861 ttggattgct gtttcttcga tactacgggt tctgctaaac gtggctagtt ccgacgttta
     1921 ataagagctt tatcgaggca acgtcacaaa caacgatacc gccgacgcac cggccttagg
     1981 cggcacgtcg gcggaaggac ctcatcgaac tgatttatgt cttgacaaag actcactgcc
     2041 caaacatcgt ctggcgaacc acgttcagat cgacgccgag aaccttttgg gcgatcgatc
     2101 tcagcgtttc gtagtcaccc tggatatgga gcgtccgatg tgcgtgctcc aatttttcgc
     2161 cggtcttaag ctcaagtgcc ggagaaagtg actcgatttc gtagaagggc cctaatcccg
     2221 gcttgtcggg cccggcagga ccatcgttat agctgttcgc tacgtctccg acgtagggtt
     2281 ctgcctgcgg gatcccccac atgttgttca tatacaagaa ctgggatgga tcctctggca
     2341 tggtgaattg caccaacgtg aggacgttgt tctggaaatc catcgatcct gccacattct
     2401 tcgctcgttt ttgcgacgta ccaatcttgg agcgataatt cccgtcgccc aagaagagga
     2461 tcgcttccgg caggattttc aggcgttccg gcgggattgg gccaaagtag tctgacttga
     2521 cgaccggtcc caaggttgcc tcatcgccct gcttgtacgg gacaataatg accgtttgcg
     2581 gcccagcgtt gaacattccc aagatccaaa tcgacacaag gccttttgcc ttgtcgaacg
     2641 gctcaccggc gttgaccacg cgattgactg tctcatatcc gaccgattta actttgcgcg
     2701 atcggacggc cgttgcaaca tctccgccga aaagccgttg gagatcgttc tcactcaaga
     2761 gacggacatc gcgggtcact tccagtcgaa actgggttcc cgccgtattg accagttcca
     2821 tcttgcggaa catgcggaag aacggatccc gcggcttaga aatgacggtc caagcgcctt
     2881 cattgagcgc cggcggggtg aaccaattat cgagaacctg atcaacgccg tgcttgaacc
     2941 agaggctgaa cggcccccct tcgggcgaga gccagaatct gtcttcgcct ccatagttgt
     3001 taaagtgggg attcggctgg cctgcatcga taaactcctt gttgataaat ccgaaactga
     3061 gtccctgcag tccatcgcag gaagacgtca tcacacgtcc ttgccattcg ggacagacca
     3121 aaacccttcc accttcctcg ttgacaagct ctaaaatctt agtatgctgt gccaaatatt
     3181 tgcgaacttg cccgtaagtc atcgccgatc ctttcccttc ctcgttcccc tgcgcgatgg
     3241 gcaagtatcc cagaaacgcg cacaaacaac aaccaaaggt caaccaccgg agctgttttg
     3301 accatttcag attcatacat cacctcatgc tgctaaaact gaagagctgc catctcgcgt
     3361 tgccacccaa ttgcgaccga gcccaaacga cttgctattt tgatctcttt tcacggctga
     3421 gtcgagatac cctggcctag cagcatatat ccctgtgccg atcaattcat ttacgccggc
     3481 acatgtttcg cttttggcac gtgcttgatg ggttactggc agcacgagtt tcagtatatt
     3541 cgcgttatgg actcactttg tggctcgaag gggacgatgc gttctctcgt ccaacttcca
     3601 ccggtgtagt ttgagggcct tcgggtacgg ctcagtcgcg ctcggcacaa aaggcctagc
     3661 acgaacagga gtctcttgat tgcccctctt actttccgcc tttgggtggg ccttcaaatt
     3721 tgcggagaca gtaggtattt gacatgcgct gtagtgttct cctaaactgt ctaacgtttc
     3781 cccgagtctt tcgtttggac aaccttcgac acatcttcag tcgatcgcgt cc
//
LOCUS       DAOPTR010000273         3817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19196_length_3817_cov_3.687135, whole genome shotgun sequence.
ACCESSION   DAOPTR010000273 DAOPTR010000000
VERSION     DAOPTR010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19196_length_3817_cov_3.687135"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1704
                     /locus_tag="PKI05_06405"
     CDS             <1..1704
                     /locus_tag="PKI05_06405"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HOA51870.1"
                     /translation="ANGDDTYISDDEDGVTFNTALTPGIDAVITVIASAAGKLDAWID
                     FNGDGDWDDAGERIFAGRDLVAGSNALTFRVPAEAVLGRTYARFRLSSTGVDAPTGFA
                     PDGEVEDYAVDITESLVQVVKQGRRVIVNGGAGNDQFELSTSSGSHSLLVNGTLYTYA
                     VADVDEITIDGQGGSDSVVFRGTTANESFVITPTTVSMTRTGFTASASNVESIQVVGN
                     GGTDSAQIYDSPADDQLVIQPNHAVMTGPGYSIDVLDVDYVHGYASTGNDKAVLHGTP
                     GNDVLTASETEARLSATGYIARAKFFKNVHVISDGAGNDVGTVTGTTGNDTIETTPSS
                     AKLTATAFQITVDQFDSVTILGNGGDDIAQLRDSAGDDWFIARPGESSLTGPGYTVVV
                     RNVPTVHAYASTGRDTAQLYDSVGNDTVVMRPDYTRITGPNYLARAKGFDYVHAYSVN
                     GFDTVKMYGSPGDDSLTVDPTFARLKGSGFYNRAKFFERIEVYGGGGYDTGVVYDSAG
                     ADHLYATSVEALLQNPDLPYYVYLTQMEKVKVRGNNPPNTKHIEGIVDWLIATGFWTD
                     I"
     gene            1977..3032
                     /locus_tag="PKI05_06410"
     CDS             1977..3032
                     /locus_tag="PKI05_06410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOA51871.1"
                     /translation="MDGSPESSNFPPATTTISVVIPVHNEVGNVAALQKELVPILESL
                     SMPWEICFVDDGSTDGTSLLLDQISHTFPNTRVIHLTRKFGQSAAIQAGLDHTHGEIV
                     VTMDGDLQNDPHDIPHLIETLQKGYDIVHGWRKHRHDHWLTRRVPSLMANWLIRKITG
                     VEIPDLGCAIRAMRRWVADCLELLGDAHRYLPIFAHSLGAKSTVVVVNHRPRQSGKSK
                     YGLSRLFRVLCDIPVVLFLTRFRSRPMRFLVTLGVINAMLVATALLLSLLTLESRSLT
                     ILAIVLATWAITTGAITTLGLGLVAELLERARTNRHPAYIVRSMSGIEHESAVIPLSR
                     ENFRSIPSHAPVKRTGT"
     gene            complement(3151..>3817)
                     /locus_tag="PKI05_06415"
     CDS             complement(3151..>3817)
                     /locus_tag="PKI05_06415"
                     /inference="COORDINATES: protein motif:HMM:NF027224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HOA51872.1"
                     /translation="TNHDISYARSSDLLHWETIDGKPIELPITPRTPGVVVDPVPVKK
                     GLINVAHYVGFDTQHRPIVTYHKYDEQGYSQIYNSRWENGRWVIRQATRWEYRWEFSG
                     GGTIQVEISSSRIHLLPDGRLAQRFSHARYGSGIWIFDPASLSVVATCPAPREIPSEL
                     AELESDFPGMEVRFARDAGKPPAGEEYVLRWETLAANRDRPRAGPLPPPTTLRLYRLQ
                     RDP"
BASE COUNT          843 a         1066 c         1069 g          839 t
ORIGIN      
        1 gccaacggag acgatacata catctccgac gacgaagatg gcgtcacttt caatacggcg
       61 ttgacaccgg gcatcgatgc cgtcatcacg gtgatagctt ctgccgccgg caaactggac
      121 gcctggatcg atttcaacgg cgatggtgat tgggatgatg ctggcgaacg gattttcgct
      181 ggacgcgatc ttgtcgccgg gtccaacgcc ctgacgttcc gcgtgcctgc ggaagcggtg
      241 ctcggcagga cctatgcacg tttccggcta agcagcacag gcgtcgacgc accgaccggc
      301 ttcgccccgg atggtgaggt cgaggattac gccgtggata ttacggagtc gctcgtccag
      361 gttgtcaaac agggacgcag ggtcatcgta aacggcggtg ctggtaacga ccagttcgag
      421 ttgagcacct caagcgggtc ccacagccta ttggtaaacg gaaccctcta tacctacgct
      481 gtggctgatg tggatgaaat cacgattgac ggccaaggcg gttctgattc tgttgtcttc
      541 cgcggtacga ccgccaatga gtccttcgtg atcactccga ccactgtcag catgacgcgg
      601 acgggcttca cggcatccgc aagcaacgtc gaaagcatcc aggtcgtggg caacggcggc
      661 accgactcgg cgcagattta cgatagcccg gctgatgacc aattggtgat tcaacccaac
      721 cacgccgtca tgacaggtcc tggttatagc atcgacgtcc tcgatgtgga ttatgtgcac
      781 ggatacgcca gcacgggtaa cgacaaggcg gtgctgcacg gcacaccggg caacgacgtg
      841 ttgacggcaa gcgaaacgga ggcccgcttg tcggccaccg gttacatcgc ccgggccaaa
      901 ttcttcaaga acgtccacgt gatttctgac ggagcgggga acgacgtggg aactgtcacc
      961 gggaccacgg gcaacgatac catcgagacg acaccgtcat ctgccaaact cacggcaaca
     1021 gctttccaga ttaccgtcga tcagttcgac tccgtgacaa tccttggcaa tggcggggac
     1081 gatattgccc agttgcgcga ttcggccggt gatgattggt tcatcgcacg acccggcgag
     1141 agcagtttga cgggacctgg ctatacggtt gttgtgcgga acgtcccgac cgtccacgct
     1201 tacgcctcaa caggtaggga taccgcacag ctttatgact cggtgggcaa tgacacggtc
     1261 gtcatgagac cggattacac ccgcatcacc ggaccgaatt acctagccag ggcgaaagga
     1321 ttcgactacg tccacgctta ctcggtgaac ggttttgaca ccgtcaagat gtacggatcg
     1381 ccaggcgacg acagcctgac cgtggatccg acatttgccc gtttgaaggg ttccggattc
     1441 tacaacaggg cgaaattctt cgaacgcatt gaagtctatg gcggtggtgg ctatgacacg
     1501 ggtgtggtgt atgattccgc tggtgcggac cacctgtatg caaccagcgt cgaggccctg
     1561 ctccagaatc cggacctccc gtactacgtc tatctgactc agatggagaa ggtcaaggtc
     1621 cgaggtaaca acccgcccaa cacaaagcac attgagggca tcgtggactg gctgattgct
     1681 accggattct ggaccgacat ctgattgttc gaaagaactt aattagggag gttaagtcct
     1741 tcaccttcac gaactcggta acagttctcc actgagacag gcgcgacgaa acggttttag
     1801 cggttgccgg tcttaccacc gacagccgct tgttttttgc ctatgttgcg cggttttcca
     1861 ggttggtgtc acggctaatt tgaacttgaa agtcactcta cggtgtcaat atacttctgc
     1921 ttctcatgga cgtcttttgg catccgggca aaataccggg aagatgggtt ttgatcatgg
     1981 atggaagccc agaaagcagc aattttcctc cagcgacgac gacaatttcc gtagtgattc
     2041 ccgttcacaa cgaggtcggg aacgtggcgg cgttgcagaa agagctcgtt cccatcctcg
     2101 aaagcctgtc catgccctgg gaaatctgct tcgttgacga cggctccaca gacgggacta
     2161 gcctcctgct cgatcaaatc agtcatacat ttcccaacac ccgggtgatt cacctcacga
     2221 ggaaatttgg acaatccgca gccatccaag ccggccttga ccacacgcat ggtgagattg
     2281 tcgtgaccat ggatggcgat ttgcaaaatg atcctcacga tattccccat ctcattgaaa
     2341 cgctgcaaaa aggctatgac attgtccacg gctggcggaa gcaccgccac gaccactggc
     2401 ttacccgtcg tgtgccttcg ttaatggcca actggctcat ccgcaagata accggcgtgg
     2461 agattccgga cctgggttgt gctatccgcg ccatgcgacg atgggtcgcc gactgtttag
     2521 aacttttggg tgacgcacat cgctacctac ccatttttgc ccacagtttg ggtgctaaaa
     2581 gcaccgttgt ggtggtcaat catcgcccaa gacagtcggg gaaaagcaaa tacggcttaa
     2641 gccggttatt ccgcgtttta tgcgatattc ctgtggtgct tttcttgacg cgattccggt
     2701 caaggccgat gcgattcctt gtcactttgg gtgtgatcaa tgccatgctg gtggcaacgg
     2761 ccctccttct ttcactactg acgttagaaa gccgttcctt gacgattctc gccattgtgc
     2821 tggccacttg ggccatcacg acgggcgcca ttaccaccct gggacttggg ctagtagccg
     2881 agctcctgga gcgtgctcga acaaatcggc atcctgccta catcgtgcgt tccatgtcgg
     2941 gaatagaaca cgagtccgca gtcatccccc tgtcgaggga gaatttccgg tcgattccgt
     3001 ctcacgcccc ggttaagcgc accgggacat aatgcgccca cgacacggga gatgtgttca
     3061 cgtttcatcg cagccttcct cttgtagcga caagctggga gcgatcggcg acctcagacc
     3121 tgtgggtttg aattaggcat caacatttca tcacgggtct cgttgcaagc gatagagccg
     3181 caatgtggtg ggtggtggaa gtgggccggc ccgaggtcga tcgcggttgg cggccaacgt
     3241 ttcccagcgg agaacgtatt cctcgcctgc aggcggcttc ccggcatcgc gagcaaatcg
     3301 cacctccatc cctggaaaat ccgattccaa ttcggccaat tctgagggaa tttcccgagg
     3361 ggccgggcag gttgccacga ccgacagact ggctgggtcg aagatccaga taccggaacc
     3421 ataacgggcg tgcgaaaacc tctgggcaag gcggccatcg ggcagtaagt gaatgcggct
     3481 ggacgatatc tcgacctgaa tggttccccc tcccgaaaac tcccaacgat actcccatcg
     3541 agtcgcttgg cgaatgaccc accggccatt ctcccatcgg gaattataga tttggctgta
     3601 accttgctcg tcatacttgt gatacgtgac gatagggcga tgctgggtat cgaaaccgac
     3661 ataatgagcc acgttgataa gccccttctt gacaggaaca ggatcgacaa cgacgccagg
     3721 cgtccgcggt gtgataggaa gctcaatagg tttaccgtca atggtctccc agtgaaggag
     3781 atcggaactt ctcgcgtagc taatatcgtg gttggtt
//
LOCUS       DAOPTR010000274         3817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19199_length_3817_cov_2.852738, whole genome shotgun sequence.
ACCESSION   DAOPTR010000274 DAOPTR010000000
VERSION     DAOPTR010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19199_length_3817_cov_2.852738"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1810)
                     /locus_tag="PKI05_06420"
     CDS             complement(<1..1810)
                     /locus_tag="PKI05_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOA51873.1"
                     /translation="MFRPVDRNPSFPKLEEGIIRFWKENRIYERSLDWRRGAPSFVFY
                     EGPPTANGLPHPGHCLTRAIKDLFPRYKTMRGYYCERRAGWDTHGLPVEVEVCKELGI
                     HSKKDIEEFGVEPFIRRCIDSVFRYTREWEQLTERLGFWIQLDNAYVTYHQSYVESVW
                     WALKQLFEKGLLYQGYKIVWWWAQGGTALSAAEVGQGYRSVADPSVYVRFPLRDEPDA
                     DLLVWTTTPWTLPSNQFAAVRPDLDYCYVLFEGEKRAVVIAQALVETVGKKVNKRYQI
                     LKTVRGASLVGKRYIPPFDYYYKRYGEQQGRLRGGGQQHIAWRVVAADFVTIDTGTGV
                     VHEAPAFGEVDYEVLLAEQARFVEGEGPQLICAVAPDGTFTAEASDYAGRWVKDCDRD
                     IIRQLRQSGVLFHFEQYVHDYPFCWRAENDPLIQYPRKSWFIRTTAFKEKMLENNRRI
                     GWHPENIRDGRFGNFLETNVDWALSRERFWGTPLPIWVCEKTGYMEAIGSYAELLAKP
                     GVSGMEVWEEAKRADPTLSEHLKVHKPYIDAIVYDSPVCPGSKMRRVPDVIDCWFDSG
                     AMPFAQWGYPHVPGSPEKLAQQFPADFISEAIDQTRG"
     gene            complement(1957..2808)
                     /locus_tag="PKI05_06425"
     CDS             complement(1957..2808)
                     /locus_tag="PKI05_06425"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOA51874.1"
                     /translation="MSETLVEGIQAAVQFIRQKWDKEAYAGIILGTGLGTLAQEIKAD
                     AVIRYEDIPHFPRSTTISHTGQFVCGTLEGLPIVAMEGRFHVYEGYTFQQVTFPVRVM
                     KALGANLLIVSNACGGMNPHYRLGDIVVIDDHINLMNGNPLIGPNDDSLGPRFPDMSQ
                     PYDQTLIDVALAIARREDFVAHRGVYVAVTGPNLETRAEYRFLRGIGADVVGMSTVPE
                     VIVAVHAGMRVLGLSVVTDMCLPDALKPAVVEEILKVAAEAEPKLRKIVLGVLRFEAE
                     KQQRRPG"
     gene            complement(2893..3252)
                     /locus_tag="PKI05_06430"
     CDS             complement(2893..3252)
                     /locus_tag="PKI05_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51875.1"
                     /translation="MNYEESPPEFDDEPVDDLDSDQEDGELPPPPPVSPEDDFVEFDE
                     DDFDDDFDDDFEFEPEYEAEIEREFGDIDQGEEIPEETEAEEEPEEPEKVLQEPGEFP
                     EGDLTDDEFTESDEEEF"
     gene            3528..>3817
                     /locus_tag="PKI05_06435"
     CDS             3528..>3817
                     /locus_tag="PKI05_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51876.1"
                     /translation="MKQLTRRRFVQLAGASLAAGILNSNRALPASLQVPGERPVRDSA
                     IRVLNPRNRVPVSLIIDDSTCLVNLAHFAMPQFAEVWPGRAEYQKPWKEFPR"
BASE COUNT          825 a         1087 c         1042 g          863 t
ORIGIN      
        1 aaccgcgggt ttgatcgatg gcctcactga taaaatcggc cgggaactgc tgggcgagtt
       61 tttccgggga ccccggtaca tgggggtatc cccactgggc aaagggcatg gcaccggaat
      121 cgaaccagca gtcgatgacg tcgggaacac gacgcatctt gctcccgggg cagaccgggg
      181 agtcgtaaac aattgcatcg atgtagggtt tgtggacctt gagatgctcg gagagggtgg
      241 gatcggcccg ttttgcctcc tcccaaacct ccatgccgct gacaccaggc ttggcaagga
      301 gttcggcata agaaccgatg gcctccatgt accctgtttt ttcgcagacc caaatgggga
      361 ggggtgttcc ccagaagcgt tcgcgcgaca aggcccaatc gacgttcgtt tcgagaaagt
      421 ttccgaaccg accgtcccgg atgttttcgg gatgccagcc aatccgccga ttgttttcga
      481 gcattttttc tttgaacgct gtggtgcgta taaaccagct cttccgggga tattgaatga
      541 ggggatcgtt ttcggcccgc caacagaagg gatagtcgtg gacatactgc tcgaaatgga
      601 aaaggacacc cgactgccgg agttgacgga taatgtcgcg atcgcaatct ttgacccagc
      661 gaccagcgta atcggaggct tcggcggtga acgtgccgtc gggggctacg gcacaaatca
      721 actggggacc ttcaccctcc acaaagcggg cctgttccgc caaaagaact tcatagtcca
      781 cctccccaaa agcaggggcc tcgtgaacga cgcccgttcc ggtgtcgatg gttacgaagt
      841 cggctgccac cacccgccag gcaatatgtt gttgaccgcc gccacggagg cgtccctgtt
      901 gttctccata gcgcttgtag tagtagtcga acggagggat gtatcgctta cctaccaacg
      961 acgcgccgcg cactgtcttg aggatttgat agcgcttgtt gaccttttta cccaccgtct
     1021 caacgagggc ctgggcaata acgacggctc gtttttcgcc ttcaaaaagg acataacagt
     1081 agtccaaatc cggtcggacg gcggcaaatt gattgctggg caacgtccaa ggagtggttg
     1141 tccaaaccag aaggtcggcg tccggctcat cccgaagtgg gaacctgaca tagacactag
     1201 ggtccgcaac cgagcggtag ccttggccga cttcagccgc tgaaagtgcc gttccccctt
     1261 gggcccacca ccagacaatc ttgtagccct gatagagcag ccccttttcg aagagttgct
     1321 taagtgccca ccagacgctt tcaacataac tttgatgata cgtcacgtaa gcgttgtcaa
     1381 gctgaatcca gaaaccaagg cgctcggtga gttgttccca ctcacgggtg tagcggaaca
     1441 cactgtcaat acagcggcga atgaacggtt cgacaccgaa ctcctcgatg tcttttttag
     1501 agtgaatgcc caactcttta cagacctcga cttccactgg taggccgtgg gtgtcccagc
     1561 cggcgcggcg ttcgcaataa tacccccgca tcgtcttgta acgcggaaag aggtctttga
     1621 tcgcccgcgt caaacaatgc ccgggatggg ggaggccatt cgccgtgggt ggtccttcgt
     1681 agaacacaaa cgagggcgcg ccccgtcgcc agtcgagcga acgctcgtaa atccggtttt
     1741 ccttccaaaa acggatgatg ccttcttcga gtttgggaaa ggatggattc cgatcaacag
     1801 gccgaaacat aagacttctc tttcgaatga accgcttcag ttagttcttt cgcacccacc
     1861 tttaaggggt ccggaggtgt acttcgccgt taagcagctt taaggtctgg tggactcgct
     1921 accaggctta tttcgagtca tggacactgc ctacggttag cccggtcggc gctgctgttt
     1981 ttctgcctca aagcgaagga cccccagcac aatcttgcgc aactttggtt ccgcctcggc
     2041 tgcaacctta aggatctcct ccacaacggc tggcttaagg gcatcgggga ggcacatatc
     2101 ggtgaccacg gacaacccca agaccctcat tccggcatgg acggcgacaa tgacttcggg
     2161 gacggtggac atccccacga catcggcacc gatcccccgc aggaagcggt actcagcacg
     2221 ggtctcgagg ttggggcccg tcacggcgac atacaccccg cggtgcgcga caaaatcctc
     2281 ccgccgagcg atcgccaggg caacgtcgat taatgtttga tcgtaaggtt gcgacatatc
     2341 gggaaatcgt ggccccaatg agtcgtcatt tggaccgatg agcgggttcc cgttcatcaa
     2401 attgatatgg tcgtcgatga cgacgatgtc gccaaggcgg taatgggggt tcatcccgcc
     2461 gcaggcattg gaaacgatca aaagattggc ccccaatgcc ttcatgacgc gaacggggaa
     2521 tgtcacctgt tggaacgtgt aaccctcata gacgtggaat cgcccttcca tggccacgat
     2581 gggtaagccc tccagggtac cgcatacaaa ttggcccgta tgacttatgg tcgtcgagcg
     2641 agggaaatgc ggaatgtcct catagcggat gacggcgtcg gctttgatct cttgagccaa
     2701 cgtcccgaga cccgtcccca gaatgatccc ggcgtatgcc tccttatccc acttctgccg
     2761 gataaattgc acggcggcct gaattccttc aacaagtgtt tcactcatag ccgatacgtt
     2821 ggttcacctc atcttgagat ctgtcaagcc aaaatcaaac tgctttccag tctttagtcc
     2881 ggtagattct ccctagaact cctcctcgtc tgattctgtg aactcgtcat ccgttaagtc
     2941 gccctccgga aactcgcccg gctcttggag gactttttct ggttcttccg gttcttcttc
     3001 agcctcagtt tcttcaggta tctcctcgcc ttggtcgata tcgccgaact cgcgctcgat
     3061 ttcggcctcg tactctggct caaactcgaa atcatcgtca aagtcatcgt cgaagtcgtc
     3121 ctcatcgaat tcgacaaagt catcctccgg cgaaaccggc gggggcggtg gcaactcccc
     3181 gtcctcctgg tcagagtcca ggtcgtcgac cggttcatcg tcaaattcgg gaggtgattc
     3241 ttcgtaattc atcggttatc ctccactttg aggtttgccg tgtatgccct ctggtgccgc
     3301 taggttggct ctctgacaat caatgaatcc aaaagacgcc cgagattgag cgtagcccga
     3361 tgcagcaaaa atacttagtt tactgcctaa gatgggctat tggtagagtg cgcggacgaa
     3421 cgacgactat ggcgatttca ggactacggc gcccctggca agggtcgttg cggtaccggg
     3481 gaccatcggc tagtgttctt ggatgaaaag ttgaggaggt ccacgccatg aaacaactca
     3541 cgcgacgccg tttcgttcag cttgccggtg cctcgctggc ggcgggaatc ctaaacagca
     3601 atcgagcgct tcccgcctcc ctccaggtcc cgggcgaaag accggtcagg gacagtgcca
     3661 ttcgggttct caatccccga aatcgggtac cggtttcgtt aatcattgat gactccactt
     3721 gcttggtgaa tctggcccac tttgcgatgc cacagtttgc cgaggtgtgg ccaggccggg
     3781 cggaatatca gaaaccctgg aaggagtttc ctcgtga
//
LOCUS       DAOPTR010000275         3814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19211_length_3814_cov_4.251929, whole genome shotgun sequence.
ACCESSION   DAOPTR010000275 DAOPTR010000000
VERSION     DAOPTR010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19211_length_3814_cov_4.251929"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1438)
                     /locus_tag="PKI05_06440"
     CDS             complement(<1..1438)
                     /locus_tag="PKI05_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA51877.1"
                     /translation="MARLSRRTFIKSSALASTAFSFAISGTRSSGQIIGANETINIGV
                     AGIRGRGSSHIDAFCAMPNVRVTYLIDPDSRLFAGRIKQVETRGGNTPKCVQDVRKAL
                     DDKDLHAISIATCNHWHSLMTIWACQAGKDVYVEKPCSHNVFEGRKCVEAARKYGRIV
                     QHGTQSRASAGWARQVAAIASGKYGKLLVSKGYASKPRWTIGFKPVKDPPPELDYNLW
                     LGPAPQLPYHENLVHYNWHWFWPTGNGEIGNQGVHQMDIARWAIPGATLPDWVISAGG
                     RFVDEPQHNWKDQGETPNMQITVMGFGDTILLFEVRGLVGKAGPGGKKFDPIVDNEFY
                     LEEGVIRNGKFYPKGKDQPEDLIKVDVKMGPGDQFENFIACMRSRKVEELHADIEVAH
                     YSSALCHLGNISYRLGKEVSFAEAAKGLPDHELVAAAWKMLEENIQGALGVELTKLTC
                     ILGPKLAFDPKKEKFVNNPEADKLLTRDY"
     assembly_gap    2304..2403
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2563..3813)
                     /gene="hydF"
                     /locus_tag="PKI05_06445"
     CDS             complement(2563..3813)
                     /gene="hydF"
                     /locus_tag="PKI05_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOA51878.1"
                     /translation="MTGYRPAPKSFRLHIGIFGRRNAGKSALLNMLTRQEVSIVSDVA
                     GTTTDPVEKPMEMLPLGPVLFIDTAGVDDEGALGEMRVQKTRQVFDRTDLALLVCDAG
                     RWEAFEEQLLQEFRQRRVPVIVVFNKMDLALPNEALCRRFEQEKIPFVFTSALRREGL
                     EAVREALIRAAPEDFINSPPLIRDLIEPGDLVVLVIPIDKEAPKGRLIQPQVMTLREI
                     LDHGAMALVVRDTELAQVFQQVPRKPSLVVTDSQAFQHVANVTPQDVSMTSFSILMAR
                     QKGDLNEFVRGALAIESLRTGDHVLVAEACSHHPITDDIGREKIPRWLQQHVGGPLRF
                     TTVQGHDFPEDLSNYRLVIHCGACMWNRREVLSRIERCRRAQVPISNYGVTIAYTLGI
                     LDRALEPFAEARELFRRLIPQEVS"
BASE COUNT          867 a          998 c         1047 g          802 t
ORIGIN      
        1 gatagtctcg ggttaacagt ttgtcggcct cagggttgtt aacaaatttt tctttcttgg
       61 gatcgaaggc cagctttggc cccaggatgc atgtgagctt cgtcagctcg acacccagtg
      121 caccttggat gttctcctcg agcatcttcc aagcggcggc cacaagttca tggtcgggca
      181 gccccttggc ggcctcggca aaggagacct ccttcccaag ccggtaggag atattgccca
      241 agtgacacaa ggccgacgaa taatgggcaa cctcaatatc ggcatggagt tcctcgactt
      301 tgcggctcct catgcaggcg atgaagttct cgaactggtc acccggcccc attttgacat
      361 ccaccttgat gaggtcttcc ggttgatcct tccctttggg gtagaactta ccgttgcgga
      421 tcacaccttc ctcgaggtag aactcgttat caacgatggg gtcgaacttc ttgccgcctg
      481 ggccggcctt tcccacaagc cctcgcacct cgaagagaag gatggtatca ccaaagccca
      541 tcacggtgat ctgcatattg ggcgtttcgc cctggtcttt ccaattgtgc tgaggttcat
      601 cgacgaagcg gccgccggcg ctgatgaccc aatcgggcaa ggtggcaccg gggatggccc
      661 aacgggcgat atccatttgg tggacgcctt ggttgccgat ctcgccgtta cccgttggcc
      721 agaaccagtg ccagttgtag tggacaaggt tttcatggta aggcaactgc ggcgcgggac
      781 ctagccacaa gttatagtcg agttctggcg gaggatcttt cacaggcttg aagccaatcg
      841 tccatcgcgg cttgctggca taaccctttg acacgaggag tttgccatac tttccgctgg
      901 caatcgccgc cacctggcgt gcccagccgg cactggctcg gctttgcgta ccgtgctgaa
      961 cgatgcggcc atacttgcgg gcagcctcca cacacttacg cccctcaaaa acattgtggc
     1021 tgcacggctt ttcgacgtac acatctttcc ccgcctggca ggcccagatc gtcatcaaag
     1081 agtgccagtg attgcaggtg gcaattgaaa tcgcatgcaa gtctttatca tcgagggcct
     1141 tgcgaacatc ctgaacgcat ttcggcgtgt ttccgccgcg ggtctcgacc tgcttaatgc
     1201 gacctgcaaa gaggcggctg tcaggatcga tcagatacgt gacccgaaca ttgggcatcg
     1261 cacagaaggc atcaatgtga ctagacccac ggccacggat tcctgctacc ccaatattga
     1321 tcgtctcatt ggcaccgatg atctgcccgg agcttcgcgt gccggagatg gcgaagctaa
     1381 aggcggtact ggccaaagcc gatgatttaa tgaacgtacg acgactcaaa cgtgccatag
     1441 tcaacctccc ctcgcacgat gcgagttatt tgggaaacag ggaaccaaac ggtcaaacac
     1501 agtttatatt gaacaccatc gtggcgggcc atacggtgag ggacttttca tccgatcctc
     1561 gatgaccccc gtcacgaatc cggtaactca tgcccgctaa gtattgaacc aagcggccat
     1621 ctgtttgtcc agtcacttgt ggcatttcaa agaattattt gccgaatgga gacaacgtcg
     1681 gtgttacctc gaatttggat caagcggctc tgcgacacag tctggtgtgg tgaacgcctc
     1741 tgattctgct tacccactac atggattggg gaaagggccc cgggattgat caagtaacaa
     1801 acgatgggcg cctgtcagtt gcgtcatatc ttcccgaggg ccgtcacggg aaacacagcc
     1861 ggaggatcgt gcgcgtgatg cgtaagctgc gattgatctt ggcaaggcaa gttgagagcg
     1921 ataatctgtt tggcgatatc tagggtaaag tgacactcaa ggaggccttc gctcgatgcg
     1981 gtacaggaac gataaagatg gagagcacaa ctgaggtgaa ccttgcacat gcgcgagaga
     2041 gaaaactgaa aaaatcgcgc gtgacggtga ttgaaatttt ttcaagatgc aatataacac
     2101 tagtgaaagc agtgcatgaa ttggtggcgc gatgaagcat tgcctaagta agaagtgcgt
     2161 ggctgggtag ttgtctgtgt gccagggcgg gaaaggtggc caagtgatgg cttcgatggt
     2221 cgaagccaac ccgccggcac ctattggacg gaggtctgaa ccgtggctga aaagaaggct
     2281 gccaagaaga cggcgaccaa gaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnaccaaga aggccgctaa gaagaagtgc tgattgggtt tcagcggctt tcaccgggga
     2461 aatgaaggaa acggacgtcc gactgacccc ggcaggttcc ggaccaagga caacgacgag
     2521 aaagggccgt gatgcaggat ggatcacggc cctagttttt tctcagctca cttcttgggg
     2581 aatcaaacgt cggaatagct cacgagcttc tgcgaatggt tccagggccc ggtctaaaat
     2641 ccccaacgtg taagcaatcg ttacgccata gttggaaatg ggaacctggg cacgacggca
     2701 ccgctcaatg cggctcaata cctcgcggcg attccacatg catgccccgc agtggatgac
     2761 gagccgataa tttgacaagt cctcaggaaa atcgtgtccc tggaccgtgg tgaatcggag
     2821 gggaccacca acatgttgct gtaaccagcg gggaatcttc tcgcgaccga tgtcatccgt
     2881 gatgggatga tggctacagg cctcagccac caacacatga tcaccggtcc gcagggattc
     2941 aatcgccaaa gcgccgcgga cgaattcatt caagtcccct ttctgtcggg ccatcaggat
     3001 agaaaaggat gtcatgctca catcttgggg cgtaacgttg gcgacatgct ggaaggcctg
     3061 actgtccgtc accacaagcg aaggctttcg cggtacttgt tggaagacct gcgccaattc
     3121 tgtatcgcgg acaaccaatg ccatggctcc gtgatcgagg atttcccgca acgtcataac
     3181 ctggggctgg atcagtcgtc ccttgggagc ctctttgtca atcggaatga cgaggacgac
     3241 cagatcgccc ggctcgatca ggtcacgaat cagcggggga ctattgataa agtcctcggg
     3301 tgcggcgcgg atcaaggctt cgcgcacggc ctccaagcct tcccggcgaa gggcggatgt
     3361 gaatacgaag gggatttttt cctgctcgaa ccgccgacat aaagcttcgt tgggcagagc
     3421 gaggtccatc ttattgaaga ccacaatgac tgggaccctg cgttgtcgga attcctggag
     3481 aagctgttcc tcgaaggcct cccagcggcc ggcatcgcaa acaagcaaag ccaagtcggt
     3541 acgatcgaag acctggcgcg tcttctgcac gcgcatttca cctaaagccc cttcgtcatc
     3601 aactcctgcc gtatcgataa aaagcacggg acctaaaggc agcatttcca tgggcttttc
     3661 gacaggatcg gtcgtcgtcc cggccacgtc cgagacgatc gaaacttcct gccgggtgag
     3721 catattgagt aaagcggatt tgcccgcgtt gcgtcggcca aaaatgccaa tgtggagacg
     3781 aaaacttttg ggtgcagggc gataaccggt cacg
//
LOCUS       DAOPTR010000276         3810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19239_length_3810_cov_2.781092, whole genome shotgun sequence.
ACCESSION   DAOPTR010000276 DAOPTR010000000
VERSION     DAOPTR010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19239_length_3810_cov_2.781092"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..1324)
                     /locus_tag="PKI05_06450"
     CDS             complement(26..1324)
                     /locus_tag="PKI05_06450"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOA51879.1"
                     /translation="MEIHRVVASVSVLGLFCLFPAQTALPEDGQPPVAALTITQPKAG
                     GVVSGNVSIVVQVMVPPGAAEPTRMLASLGGVPWVELTKDGDKWQATLNSTLVPNGPQ
                     LLKVVTNNRKVQASCTVNVDNPLHCWFADLHSHTSYSDGTYLPSDAHAYARNVAKLDV
                     FSLTDHLESVDANEWQDIRETAWKANELGKFVAIVGLEWTKAWGHLCIYDPPTYRWPT
                     DPQAFYQAAVEANVVLKFNHPGDGTKSHDGLAYSELGDKVVQLMEVRNPVEEQAFQRA
                     LRLGWHIAPEGSTDTHGPNWGNTNTWTGILAPGLSVRNILDALANRRCYSTRDRNCRF
                     QFSINGSPMGTIITTPIQKAKLTIQLNDPDDNDVITKVELFQDGELVDVFEPNQKDVL
                     LEKTLTPSEGKHYIWAKVTQADGNLLWGAPIWFIASGKAE"
     gene            2128..3459
                     /gene="tatC"
                     /locus_tag="PKI05_06455"
     CDS             2128..3459
                     /gene="tatC"
                     /locus_tag="PKI05_06455"
                     /inference="COORDINATES: protein motif:HMM:NF013097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOA51880.1"
                     /translation="MTFGEHLEELRQCLFRAIWGLAIGVVIGLIFGNRVVALIQTPLE
                     KALTKYYVGQSHREVTKQIQRLSEQGVELPFSAEAAAKFVIDHQVLAEEFLISPRELV
                     TQFELSLKEMTRRWMADKGSLEQALNVLQKMSASPDSKELEVTDKAVITEAVQILRRH
                     IGPGTLSEASEAFGGLIDLADKLAAGEQLSAEDSHLLRNGLQRMIGQLASAIESVQNT
                     NQALAGLVLATRQEPGSFEQSELMHVFLWRPAKEDPRIRAKSLSAHEAFSIYMKASFL
                     LGAIISSPWVFFQIWSFVAAGLYRHERRFVYIFLPFSLALFLAGATMAFVYVFEPVLT
                     FLFQFNEWLGIQLEPRISEWLSFVLILPLGFGIGFQLPLVMLFLERIGIFTLQDYASQ
                     WRIAVVVIFVISAILTPPDPSSQLLMAIPLCLLYFGGMALCKFFPRKKENR"
BASE COUNT          869 a          965 c         1025 g          951 t
ORIGIN      
        1 gagggggact ttttgggata cactcttatt ccgccttgcc agacgctatg aaccagattg
       61 gcgcgcccca caaaagattc ccatcggctt gagtcacctt ggcccaaata tagtgcttcc
      121 cttccgaggg cgtgagggtc ttttccagga gcacatcctt ttgatttggc tcgaaaacat
      181 cgactagttc gccatcttga aacagctcga ccttggtaat gacgtcgttg tcatcaggat
      241 cattaagttg gatagtcaac ttggcctttt ggataggagt tgtgatgatt gtgcccattg
      301 gcgacccgtt gatgctaaat tggaaccgac agttgcggtc gcgcgtggag taacatcggc
      361 ggttggctag ggcatccaag atatttcgaa cagaaagacc aggtgccaga atgccggtcc
      421 aggtattggt atttccccaa tttggaccgt gggtatcagt ggaaccttct ggcgcgatat
      481 gccagcctaa ccgcaatgca cgctgaaagg cctgctcctc tacagggttt cgaacttcca
      541 tgagctgtac aaccttatcg cccaattcgg aataggccag accgtcgtga ctctttgtgc
      601 cgtcacccgg atggttgaat ttcaaaacga cattggcctc aactgccgct tggtaaaagg
      661 cctggggatc cgttggccaa cggtaggtcg ggggatcgta aatacataga tgtccccatg
      721 ccttggtcca ttccagaccg acgattgcca caaattttcc cagctcattg gccttccagg
      781 ctgtctcgcg gatgtcttgc cattcgttgg catctaccga ttcaaggtgg tctgtcaagg
      841 aaaacacatc gagcttggcg acgtttcggg cataagcatg ggcatcgctc gggagatagg
      901 tgccgtccga atatgatgta tggctgtgaa gatcggcgaa ccaacagtgg agaggattat
      961 cgacgttgac ggtgcaactg gcttgaacct tgcgattgtt cgtgacaacc ttgaggagtt
     1021 gaggtccgtt gggaacgagt gtgctattaa gagttgcctg ccacttgtcg ccatctttag
     1081 ttaactcgac ccaaggcact ccgccgaggg aggcaagcat tcttgtgggc tcggctgcgc
     1141 ctgggggcac catgacctgc acgacaattg atacgtttcc ggacacaaca cctcctgctt
     1201 ttggttgagt gatggtgagg gcggctacag gcggttgtcc atcttcgggt agcgccgtct
     1261 gcgccgggaa caaacagaaa agacctaaca ccgaaacact cgctactacc cgatgaattt
     1321 ccatgggttt ctcctgtctt cttctggaac ctccctacag gtgcacaatt cgcttcgagc
     1381 aatattctca tgtgcaatgg gtgcgaaatc aatacggttg acttgtttgc aggatttcta
     1441 ggaaacgctt gatatctggt ggattgccga caccgaaatg ttggagatac agtgctccgt
     1501 ggtatctatt cgttcgtacg ccagctgggg ttgagccact tttgacgctg taacacgcca
     1561 gaaaacggca ggtaatggag gaatgcttgg gccacgcagt accggaagtg aatccagagc
     1621 ccacgtgcat ttggaatacg aaaatggggc acaatacatg caagtcgcag cgaaactaca
     1681 aaacggcaaa gtatatatct cagagcctat atcccaaaat tccctctccc tttgggagag
     1741 ggttggggtg agggtaaggc atcgtcggag tcgatcgaga aaaccagcag cgattcgagc
     1801 caccctcacc ccggccccct cacgccgaac gggagaaggg aggttgtggg ataggcgcta
     1861 aatacgcaaa acgcacattt gaagccagcc gtgctcgcaa gctttatccg aaatgtgcgc
     1921 cgtggcttcg aaaaaagtgc gtgaacccct gggacgccag cgattgcccg tgtcagtagg
     1981 cgctgataag atagcaaaga tgcgcccacc gtgggcgagc cccgcgaacg acaacgtcgt
     2041 acggatcact ttgaatagtc gttgtgctca atgatacgtc tattaccacc ccccaaatcg
     2101 gacgaagatc tctttcgtga atcaacgatg acattcggcg agcacttgga ggagctccgc
     2161 caatgtcttt ttcgtgctat ttggggactg gcaatcggtg ttgtgattgg tttgatcttt
     2221 ggcaaccggg tcgtggcgtt aattcagacc cccctggaaa aggcccttac taaatactac
     2281 gtgggacaat cacaccgaga ggtgactaag cagattcaac gattgtcaga acagggggtc
     2341 gagttgccct tcagtgcaga agccgcagcg aagtttgtca tcgatcatca ggtattggcc
     2401 gaggagttct tgatttcgcc gcgtgagctt gtcactcaat tcgaattgtc gctcaaggaa
     2461 atgacgcgac gatggatggc agacaagggc agtttggagc aggcactgaa tgtgctgcag
     2521 aagatgtcag cgagtccgga ctcgaaagag ttagaagtaa ctgacaaggc ggtgattacc
     2581 gaggcggtgc aaattcttcg gcgtcatatc ggtccaggta cattaagtga ggcctctgag
     2641 gcgtttggtg gattgatcga tctagctgat aagttagcag cgggagaaca gctttcggca
     2701 gaagatagcc acctgttgag aaacggcctc cagcgcatga tcgggcaatt ggcgtcagcc
     2761 atcgaatccg tccaaaatac caaccaagcc ttagcgggat tggtgttggc aactcggcag
     2821 gaacccggtt ccttcgagca aagcgaactc atgcacgtct ttttgtggcg accggccaaa
     2881 gaagatccac ggattcgggc gaaaagcctg agtgcacacg aggctttttc tatctatatg
     2941 aaggcttcct tccttctggg tgccattatc tccagccctt gggtgttctt ccagatttgg
     3001 tcatttgtgg cggcaggtct ctaccggcac gagcggcgtt ttgtctatat cttcctgccc
     3061 ttcagtctgg ccctatttct ggctggggca acaatggcgt tcgtctatgt gtttgagcct
     3121 gtcttgacgt tcctcttcca atttaacgag tggttaggga tccaactgga accccgaatc
     3181 agcgaatggt tgtcatttgt cttgatcctc ccattgggct tcggaatcgg gtttcaattg
     3241 ccgctcgtta tgctctttct agaacgaatt ggcatcttca ctttgcaaga ttatgcttct
     3301 cagtggcgga tcgctgtggt ggtcatcttt gtcatctcgg ccattttgac gccgcccgac
     3361 ccctctagcc aactcctgat ggccatacca ctatgcctgc tctactttgg cggcatggcc
     3421 ctatgtaagt ttttcccccg caagaaagaa aaccgctgag agagcagcag ctttctatct
     3481 gaccgtggcc cgttgacgcg aatcttgcca aagtttccct gaagattcag ccctgtgcgg
     3541 cagccggcta ggcctaccaa atccaattgc gtgtggcttt ctggtgcaaa tccaacgtag
     3601 ataagaagta tagtaggtgc ctcgccactg gccggcattg gctccgaggt tttcgaaccg
     3661 tgttgcaaag tttatctatc tatggtgtgc ttaccaccca caccttgtcg atggcctcac
     3721 tcagatcgcc gcccgtttca acctgaatcg agagcgtcgt atcattgggc tctgcgacaa
     3781 actcgatttc gtatcgagat tcatcctcag
//
LOCUS       DAOPTR010000277         3808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19252_length_3808_cov_3.471090, whole genome shotgun sequence.
ACCESSION   DAOPTR010000277 DAOPTR010000000
VERSION     DAOPTR010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19252_length_3808_cov_3.471090"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PKI05_06460"
     CDS             <1..262
                     /locus_tag="PKI05_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51881.1"
                     /translation="EGANTLPIAKPLVETATIPHELVDQAGDEAEASPFPLPLPADED
                     PRLALVLGGRARRSAPPMPFWIWIIIALGLVTAVVLAIIQFV"
     gene            409..873
                     /locus_tag="PKI05_06465"
     CDS             409..873
                     /locus_tag="PKI05_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOA51882.1"
                     /translation="MKERHKILIADDNPTNVELLEAYLADYDCEIAVASDGLETLAMA
                     RKIHPDLILYIMMPKMSGFEVCQKLKSDPATKDIMILMVTALNELGDIERAVAAGCDD
                     FLSKPIHKLELLKRVENMLKLRQVTDELARLRAYIDAMEETRPGQRGPAKGY"
     gene            complement(887..960)
                     /locus_tag="PKI05_06470"
     tRNA            complement(887..960)
                     /locus_tag="PKI05_06470"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(924..926),aa:His,seq:gtg)
     gene            complement(1365..2249)
                     /locus_tag="PKI05_06475"
     CDS             complement(1365..2249)
                     /locus_tag="PKI05_06475"
                     /inference="COORDINATES: protein motif:HMM:NF026067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4465 domain-containing protein"
                     /protein_id="HOA51883.1"
                     /translation="MSSLAQTPLARSCLWLPSLFVLGLLVPLNSIQAGTVDFEDVGAS
                     LPANSYWNGAPNDGHNTFTSNGFIFHNNYTLAWDVWDGFAYSNKTDTTTRGYLNQYSA
                     ITGSGADNSATYAVSFVNLWGDLPRIEVPEGVSLVSAQVTNTTYAYYSMLEGDQFAKK
                     FGGPSGNDPDWFKLIIYGKDLANSLLGTVQFYLADFRFEDNAQDYIVNTWQTVDLTPI
                     STARILEFELDSSDVGPWGMNTPACFALDNLVFQTPGSNGEVIPEPTSVVLLGLSTVA
                     IGGVSVLRRWRRREKDQV"
     gene            complement(2322..3191)
                     /locus_tag="PKI05_06480"
     CDS             complement(2322..3191)
                     /locus_tag="PKI05_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOA51884.1"
                     /translation="MDRRSALLRNRCGERTLRLFSRVIRKGFTLVELLVVIAIIGLLT
                     ALLLPAVQAAREAARRSACTNNLRQMGIGLHNYHQALGCFPPGGIEHRAMINPQTKKP
                     YGPAGRQLAWSLFLLPYVEQQPLYQKVDTSKPFDAPENAEAAATILPLYICPSATTQR
                     ELRQGRGPCDYGGIYGERITSPNNPPKGMMLYDQALTVADVIDGTSNTLIVSEDSDFR
                     DGQWINGLNVFDQAYAINKAPAFENDIRSKHPGGANGLFTDASVRFLAETMDLSVLAA
                     ICTRAGGEVVTGL"
     regulatory      complement(3351..3599)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          893 a         1039 c         1046 g          830 t
ORIGIN      
        1 ggaaggggcg aacacactgc cgatcgccaa accccttgtc gaaacggcga ccatccccca
       61 tgaacttgtc gatcaagcgg gggatgaggc ggaagcgtct ccctttccct taccactgcc
      121 tgcggatgaa gacccacgat tggccttggt gcttggcgga cgggcccgac gcagcgcacc
      181 cccgatgccc ttttggattt ggattatcat tgccttgggg cttgtgacgg ccgtcgtttt
      241 ggccatcatt cagttcgtgt gatggcgaat cctccgctgg ttcgtgattc gccttttgcc
      301 gtcgagaatg aggttctata attcggcaaa gcaggcggtt ttcaagattc cctagaactt
      361 tcggcgatgt ttttggaccg ggcttaactt cgacttggac aagtcagtat gaaagaaaga
      421 cataagattc tcatcgcaga tgataacccg acgaatgtcg agctattgga ggcctatctg
      481 gccgactacg attgcgagat tgccgtggcc agcgacgggc tggagaccct tgccatggcc
      541 cgaaagattc accccgacct catcctctat atcatgatgc ccaagatgag cgggtttgag
      601 gtgtgccaga agctgaagag cgatcctgcc accaaggata tcatgatcct tatggtgaca
      661 gcactcaatg agttggggga catcgaacgg gcggttgcgg cgggctgcga tgatttcttg
      721 agcaagccga ttcacaagct ggaactactc aaacgcgttg agaatatgct aaaactgcga
      781 caagtgaccg atgaattggc acggcttcgg gcctatatcg acgccatgga agaaacccgc
      841 ccaggccaac gcggtccagc caaagggtac taacacttgc cctaagggtg gctgacggga
      901 ttcgaacccg cgacacccag atccacagtc tggtgctcta accagctgag ctacagccac
      961 catatcaggg atatttgccg ccccgcgatg accgtgacgg ataaggctgt ggtccgtcgc
     1021 ggaagtcaac gaccaggagc tagccgtcaa tcacgggacc agtcatcaat acttttagtc
     1081 tatccggcct ctcctgcgcg gtcaacgatg ggtggcactt gaccggcgtg cttgcgtaac
     1141 taacgtttgt aaaaggattt caggtcattt gctgcccaag ggcttgggct acactcatgc
     1201 cttagaccaa tggtattcca aaacaaccca gaccccgggg aggggaaccc ggggtctggc
     1261 gacgacgtcg cagggacgtc ctcggagggg tttttcggaa attgtgtcgg gctaattgcg
     1321 attcccaaat tcaatcaggt catgtaaggt actcaggcat cggctcacac ctgatctttt
     1381 tcacggcgac gccaccttcg gagcacggat acgccgccta tggcgaccgt cgaaaggcca
     1441 agaagaacta cgctcgtcgg ctctgggatg acttcaccgt tactgcccgg cgtctggaag
     1501 actaaattat ccaaggcaaa acacgccggg gtattcatac cccaagggcc cacatctgag
     1561 gaatcgagct caaattccaa aatgcgggca gtgctgatag gtgtcaaatc gaccgtttgc
     1621 caggtattca cgatgtagtc ttgcgcgtta tcttcaaagc ggaaatcagc gaggtagaac
     1681 tgaaccgtcc cgagtaagct attggcaagg tcctttccgt aaatgatcag tttgaaccag
     1741 tcgggatcgt tgcctgaggg gccaccgaat ttcttggcaa attgatcgcc ttcgagcatc
     1801 gagtagtagg cataggttgt atttgtcacc tgggcggaaa cgagggacac accttccggg
     1861 acttcgattc tgggcaagtc accccacaaa ttgacgaagc tcacggcgta ggtcgcagag
     1921 ttgtcggccc ccgatcccgt aatggccgaa tactggttca gatacccacg agttgttgtg
     1981 tcggtcttgt tggaataggc aaatccatcc cacacgtccc acgccagggt gtaattattg
     2041 tgaaagatga atccattcga agtgaacgta ttgtgaccgt cattgggggc gccgttccag
     2101 taactgttgg ccggaaggga ggccccaaca tcctcaaaat cgaccgtacc ggcctggatc
     2161 gagttcaagg gaaccagtag tcccaaaacg aacaggctag gcagccagag gcacgatctg
     2221 gccaagggag tttgtgcaag cgaactcatg atgctcctcc tttgttgaaa cgtaaaccaa
     2281 caaaacacgc caacatagtg ttcctgcttc gataaaccgg cttacagccc ggtgaccact
     2341 tcgccacccg cacgtgtgca gatggcggcc aaaacgctca aatccatcgt ctctgccaag
     2401 aaacggaccg aggcatccgt aaacaggccg ttggcaccgc ctggatgctt gctgcggata
     2461 tcgttttcaa acgcgggtgc cttattaatg gcataggcct gatcaaaaac gtttaggccg
     2521 ttgatccatt gaccatcgcg aaagtcggag tcctcggaga caatgagcgt atttgatgtt
     2581 ccatcgatga catctgccac agtaagcgcc tgatcataga gcatcatccc tttgggggga
     2641 ttgttgggac tggtgatgcg ctcgccatag atacctccgt aatcgcatgg tccgcgaccc
     2701 tgccgcagct ctcgttgcgt tgtggcactt gggcaaatat agaggggcaa gatcgtggcg
     2761 gcggcctcgg cattctcggg tgcgtcgaac ggtttactcg tatcaacttt ctggtagagt
     2821 ggctgttgtt caacatacgg gaggagaaat agcgaccagg ctagttgccg ccctgccggt
     2881 ccatagggtt ttttggtttg tggattgatc atggcacgat gttcaatccc gcccggcgga
     2941 aaacatccta aagcctgatg gtagttatgc aggccgatgc ccatctgccg caaattattt
     3001 gtgcaggccg agcggcgggc ggcttcacgg gcggcttgaa cggccggcaa aagaagcgct
     3061 gtcagcagcc caatgatggc aattaccacc agcaactcga caagtgtgaa gcctttccgt
     3121 atcacgcgag aaaatagccg taacgtcctc tcgccgcacc tattccgaag cagggcagag
     3181 cgacggtcca tcgcaaacct cctgccgcta tcgtgcagaa agcaacccta gcgctactgg
     3241 gaacatactg tccccacgcg tggccgaaac ttaatgtggc tgatgagttg caacctgctc
     3301 gatagcctca agtaaggtcc agaactccgc agcctccgtc ccacgcagag gactgacagc
     3361 catgcgttgg taggtcttct gacttccgag ctggactcac ccggccttct cgtcgcaacg
     3421 attcctcatg ggattagacc attaggaacc tgctggctgc cgcgacaatg gccaaggaga
     3481 ctgggcaagt ctcgtggcgc tcggttacag cggcggggac cgttccggat tctcaccgga
     3541 ttccctgttc tccgatcagg ctagctgccc gaccggacac caacgcccaa cacggaccat
     3601 cctagcgttg ctacctgccc gtgtcaactg cccaagattc caaaatcgaa acgcccagct
     3661 tccttggatt caaaatgatg acaagatcag tacaattaac ggagctggga gaaagagcat
     3721 cgcaaggatg gcccttcgtg ggcgtttgtt ttaacccgta ttgcagtcgt cccagggcgc
     3781 ctcaaacaaa ggggggagat tatggatc
//
LOCUS       DAOPTR010000278         3806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19267_length_3806_cov_3.977073, whole genome shotgun sequence.
ACCESSION   DAOPTR010000278 DAOPTR010000000
VERSION     DAOPTR010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19267_length_3806_cov_3.977073"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="PKI05_06485"
     CDS             complement(<1..839)
                     /locus_tag="PKI05_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA51885.1"
                     /translation="MRGAPIATGFLDRLRRWPALTLTLSQRERELWDRLLAATLLFWC
                     IACYTTCAEDSSPRSPLRWAADAEGGAPYIFQDPNNPQQVIGFEVDLAKALAEKLGRP
                     VEWVQYDYMSLVAGLQRGDFDFAMNGLEVTPDRAKVVRFSRPYYVYTLQLVVRANETR
                     FSNLDELLALGGTVGTLEETAAQRLLEGMGFQPKLYGNQTEPYLDLELGRIDAVLMDL
                     PIAVYFAKPNPKLKFLGEPLGEGYYAIAFRPEDEELAVAVDQALTELIAEGQIRAIYE
                     KWGL"
     gene            complement(1283..2959)
                     /locus_tag="PKI05_06490"
     CDS             complement(1283..2959)
                     /locus_tag="PKI05_06490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HOA51886.1"
                     /translation="MLPALLAMFPLLLVFVLLVGWQWPARRTMPLCYVATLIIGAFYW
                     QMTWSRILAATVEGLIIALMLLYIIFGALLLLAMLKHSGAVSAIRDGFRKISPDRRIQ
                     AVIVGWAFGSFIEGASGFGTPAAVAGPLLVILGFPPLAAATATVTIQSTPVSFGAVGT
                     PILVGVNKGLQDQPTVLTYLAEQVSGQGLSPAAAFEQLLYSIGCRVAIIHGLIGLLIP
                     FFVVCLLTRFFGARRSWREGLEVWKFCLFSGLAFCVPYVTFAWVLGPKFPSLLGGPVA
                     LILTTLAARAGWFQPKKPWDFAESPTENPGHRAVGEAVTASWNAGVWWAWLPYVVVAG
                     LLVLEQWNPIKNTLARANWEWTIAASEVTSQAIVARWNILASPGTAFFLAVLFTIVLF
                     AIPRESLQKAFGEAGQKTFAAALALVFAVPAVRIFIQSNVNGAGLASMPQELAAAVSQ
                     CVGGVWPAFAAGIGALGAFIAGSNTISNMTFALFQFEVALRSSLDPLWIVALQAVGGA
                     AGNMVCVHNVVAAAATVGIIGQEGSIIRKTMIPTIYYLIAAGILGLAFCH"
     gene            complement(2992..3222)
                     /locus_tag="PKI05_06495"
     CDS             complement(2992..3222)
                     /locus_tag="PKI05_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51887.1"
                     /translation="MGRHKVVPYAWLENLLATTTRDNVTVGASDYTIGAGRYGAGTGA
                     CPYRDGCNAGTSLRTPGSSNYHDKALSVISLA"
     gene            complement(3612..>3806)
                     /locus_tag="PKI05_06500"
     CDS             complement(3612..>3806)
                     /locus_tag="PKI05_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51888.1"
                     /translation="LMPDRPLREVTRPFLRVLPTERLVSVLQLMYQHGQKLALVIDGK
                     DQAVGVVWEQELRRFCLTSS"
BASE COUNT          904 a         1157 c         1078 g          667 t
ORIGIN      
        1 aggccccatt tctcgtagat cgcccgtatt tgaccctccg cgattaattc cgtcagggcc
       61 tgatcgaccg ccaccgcgag ctcttcatct tcgggacgga aggcaatcgc ataatatcct
      121 tcccccaggg gctccccgag gaacttgagc ttaggatttg gcttagcgaa atagacggca
      181 atcggcagat ccatgagaac ggcatcaatc cgccccaatt ccaggtcgag ataaggctcc
      241 gtctgattgc cgtacagctt gggttggaag cccatcccct ccaggaggcg ctgggccgcc
      301 gtctcctcca gcgtgccgac cgttccgccc agggcgagaa gttcgtccaa attgctgaag
      361 cgggtttcat tagccctgac cacaagctga agggtataaa catagtacgg tcgcgagaac
      421 ctcacaactt ttgcccgatc tggcgtcacc tccagaccgt tcatggcaaa atcaaagtcg
      481 ccccgctgaa gacccgccac aagactcata tagtcgtact gaacccactc cacggggcgg
      541 ccaagttttt ccgcaagggc cttcgccaga tcgacctcaa aaccgatcac ctgctgcgga
      601 ttattggggt cctgaaagat atacggggca ccgccttcag catccgccgc ccagcgcagc
      661 ggcgaccgag gtgatgagtc ctcggcacat gttgtgtagc aggcgataca ccaaaaaagc
      721 aaagtcgccg ctaagagcct atcccaaagt tccctctccc gctgggagag ggttagggtg
      781 agggcaggcc atcgtcggag tcgatcgaga aaaccagtgg cgattggagc ccccctcacc
      841 cccggtccct ctcccggcga acgggagagg ggtgattgag ggataggctc taaagtccca
      901 acgcgtataa cccaggacgt tatccgatcg ctcaatcttg atttcgaaga catgaaccga
      961 ctgcggttgt ttgcgaaatt tgcacggtcc gcgtggtgtt ccaggacaaa gtcagcggcc
     1021 gacggttctc tttagacgct cgagaaaccc cacattgtcc caaggatatc ctgtcggtac
     1081 gtcttctcgc aagttgcctt gagtgacatg gtggacgcga aaaaatgcgt ttttccagaa
     1141 tgtcggacgc gggggaccgc gtaactcgca tacggcagga cgcgaggacg gcgtccctcc
     1201 caaacgacag gacgccgggg acggcgtccc tcccgcgtga aaaaacacgt ttgacgccag
     1261 tgagcgcgtc cctccctagg ggtcagtggc aaaaagcgag tcccaagatg ccggcagcta
     1321 tgagatagta gatcgttgga atcatggttt tccggatgat gctcccctcc tggccgataa
     1381 tccccaccgt ggccgcagca gccaccacgt tgtggacgca caccatattt ccggcggccc
     1441 cgcccacggc ttgaagggcc acaatccaaa gggggtccaa actgcttcgt agggcgactt
     1501 caaattgaaa gagggcaaac gtcatgttgc tgatggtgtt gctcccggcg atgaaagccc
     1561 caagtgcccc gatccctgcg gcaaaggctg gccaaacccc tccgacgcat tgggacacag
     1621 cggcggccag ctcttgcggc atgctggcca atcctgcccc gttaacattt gactggatga
     1681 aaatccgcac ggcagggaca gcaaacacca gggccagtgc ggcagcaaac gtcttttgcc
     1741 cggcttctcc aaacgctttc tgtaagcttt cgcggggaat ggcgaacagc acgatggtga
     1801 acagaacggc gaggaaaaag gccgtccccg gcgatgcgag gatattccag cgtgccacga
     1861 tcgcctggct ggtgacttct gaggcggcaa ttgtccattc ccagttggcc cgcgcaaggg
     1921 tgttttttat cggattccac tgttccaaga ccaggagtcc cgccacaaca acatagggaa
     1981 gccatgccca ccaaacgccc gcgttccacg acgccgtgac ggcctcaccc acggcccgat
     2041 gccccggatt ctccgtcggg ctttcggcaa aatcccatgg tttcttgggt tggaaccagc
     2101 cggcgcgggc ggccagggtg gtcaagatga gggcgaccgg tcctcccagg agcgaaggaa
     2161 atttggggcc taggacccag gcaaaggtca catacggaac gcaaaacgcc agaccggaaa
     2221 agaggcagaa cttccacacc tcaaggcctt cccgccatga gcgacgggcc ccaaaaaagc
     2281 gggtcaacag gcagaccacg aaaaacggga tgaggagccc aatcagcccg tgaatgatgg
     2341 caacgcggca cccaatgctg tagagcaact gctcaaaagc cgcggcgggg gacaggcctt
     2401 ggcccgacac ttgctcggcg aggtaggtaa gcacggtcgg ttggtcctgc aggcccttgt
     2461 tgacaccaac gaggatggga gtgcccaccg caccaaagga aaccggcgtg ctttggatcg
     2521 tgaccgtggc cgttgcggcc gccaagggcg gaaacccgag gatcaccaag aggggaccgg
     2581 ccacagccgc cggtgtgcca aatccggagg ccccttcaat aaaactaccg aatgcccaac
     2641 cgacaatcac cgcttgaatg cgacgatctg gagagatttt ccgaaagcca tcgcgaatgg
     2701 ccgagacggc ccccgaatgc ttcagcatgg ccagcaacag caaggcccca aagatgatat
     2761 aaaggagcat cagggcaata attaggccct ccaccgtggc cgccagaatc cgcgaccagg
     2821 tcatctgcca ataaaacgcc ccgatgatga gtgtcgcgac atagcacagc ggcatggtac
     2881 gccgggccgg ccattgccaa cccacgagca gcacaaaaac cagaagcagg ggaaacatcg
     2941 ccaaaagtgc aggtaacacg ctcgtcatca cctcttccaa aagaccaaga atcatgcaag
     3001 cgatatgacg ctgagggcct tatcatgata attcgacgaa ccgggcgttc tcaacgaggt
     3061 ccccgcgttg cagccatccc ggtaggggca ggcccctgtg cctgccccat aacgtccagc
     3121 cccaatcgtg taatcgctag cccccaccgt gacgttgtcg cgtgttgttg tcgccagcag
     3181 gttttccagc catgcgtagg ggacgacctt gtgcctgccc attcgtgaag agtctatgcc
     3241 aaaaagtccc cctctccctc cgggagaggg ttggggtgag ggcaagcgat cgtcggagtt
     3301 gatcgagaaa accggcagcg attcgagcta ccctcacccc cgcccctctc ccgccgaacg
     3361 ggcgagggga gattatggga ttggctcaaa agcaaaaaag agcgaccaca agggttgcgc
     3421 ctgccagcga tggaggacca tggcccctcg tggccgcgcc aatctatagg gttatgggcg
     3481 ctacccattg ggtatcccca acaaggattg gaatacgcac agcgatagga ccaggcagac
     3541 gagatgacgc atggtctatc gaaaaccatc acccgcttac gccggcggct cgggatgaca
     3601 atcggcacag gtcatgacga agtcaggcag aaacggcgca actcttgttc ccaaacgact
     3661 cccaccgctt ggtccttgcc gtcgatcaca agggccaatt tttgaccgtg ttgatacatc
     3721 aattgcaaca cgctcaccag tcgctcggtg ggaaggactc gcaaaaaagg tcgcgtcacc
     3781 tcgcgcaggg gacgatcggg catcaa
//
LOCUS       DAOPTR010000279         3801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19289_length_3801_cov_3.392952, whole genome shotgun sequence.
ACCESSION   DAOPTR010000279 DAOPTR010000000
VERSION     DAOPTR010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19289_length_3801_cov_3.392952"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PKI05_06505"
     CDS             complement(<1..325)
                     /locus_tag="PKI05_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51889.1"
                     /translation="MRRPSSKPATISRQLPRMNYVVGMLLLSGLAITAVGCAKFDLRR
                     NIPWQETSDKPVRSVVAFWTEAVIERTSGPPYRGFGGRLLFYPADMSHPVKGQGTVVV
                     YDFDED"
     gene            complement(386..>3801)
                     /locus_tag="PKI05_06510"
     CDS             complement(386..>3801)
                     /locus_tag="PKI05_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOA51890.1"
                     /translation="SGSPANLPAGQAQQSGTGPSSSGRSFPVQMTYASAEMDRDTSQM
                     GSSLTIEEIPLWNLKATQLEAALQSMLGSTLEPINVVPQAARAEYQMALHTGEKLGLV
                     VDRINHRIRVTGPMWSLMPARQLIQALDSPASSAGQTVRLIPLANARSSTVHQAAQAI
                     QAANSTQQEAENRLPQDLVVTQVEPSQPPTPQPSVTTTQEQPQGQQPSAPQPGVTQPP
                     AVQAPPAEPPAPGEAAPGGAAPEEGGGLVGPVQIEFLEGLDVLVVRGNRRDVERVMQI
                     IRQIEEISVETEPEIRIALLRHVDCEDLLAILTQVYQSVYAARRGSITMIALVKPNAI
                     LLVGRQEAVNTVLELIAKLDRPVAPESEFKVFPLRHASATTIEQRLTTFYANRGGLGT
                     KILITSDFRSNSVIVKASPRDMLEIEQLIAKLDTPTAEAVNEIKVFTLKNSLASELAP
                     ILQQAISAQAAGALRQTATGQLTVQAGAQAQQEIRSTRLRFVTVDTEGKQLLESGIMT
                     DVRITADTRANALVVAAPAESMPLIEALITELDRLPSAEAQIKVFTIVNSDAQALADM
                     LQTLFGQAVTAAAGAAAQPAYRTGAQEGESSLIPLRFAVDVRTNSIIASGSAGDLAVV
                     EAILLRLDESEVIKRKTQVFRLKNAPANDVANAINSYLTNERQVRQLNVNVFSTFEFL
                     SKEVVIVPETVSNSLIVSATPEYFDEIVEIITQLDERPPMVLIQVLIAEVQLSNTDEF
                     GIELGIQDPILFDRSVAGVPGFLFNSTGALGNNTTAPGSDIVGAQAISNFGVGRGNST
                     LGFGGMVLSASSDAVSVLIRALRQKRRLDVLSRPQVMALDNQAAFIQVGQQVPTVTGT
                     SVTQYAQVNSVSYTNTGLILLVRPRISPDGQVVMEINAEKSDVGPESEGIPISISAAG
                     QIIRSPRINTTRAQTTISAGDGQTVILGGLITKSRSMTNRRIPGLSDVPILRHLFRYD
                     LGDASRTELLIFLTPHVVRTDEDVERARQIEAARMHWCLADVVALHEDLAPSPTSQSP
                     TPAVPPGPAPQGIQPSQEAIPTPPPSLPNNQGQSQPRSTPGNANWNAVQQPVFFDTPM
                     ESPVDSKWQADVGTSHISDQPGRDNGAVVDENSQLAWPTPAYPSSGTRR"
BASE COUNT          763 a         1016 c         1142 g          880 t
ORIGIN      
        1 ggtcttcatc gaaatcatag acaacgaccg ttccttgacc cttgaccggg tggctcatgt
       61 ccgcggggta aaaaagcagg cgaccaccaa aaccccgata tggtgggccg gacgttcgtt
      121 caatcacagc ttccgtccaa aaagccacga cagagcgaac gggtttgtcg gacgtctctt
      181 gccaggggat attccttcgt aagtcgaatt tggcgcaccc gactgcggta atcgctaggc
      241 cactcagtaa taacatgcca acaacgtaat tcatacgtgg caactgccgg gagattgttg
      301 cgggtttact gctgggacga cgcatagtaa ggctcctcac gggtgtctct cgcctcttgg
      361 ttttagttcg tttgttcacc ctgacttagc gccgtgttcc agaagaagga taggcaggtg
      421 ttggccaggc cagttgactg ttctcgtcga ccacggcccc attatctcgc cctggctgat
      481 cggaaatgtg tgatgtacca acgtcggctt gccacttgct gtcgacaggg ctttccatgg
      541 gagtgtcaaa gaacaccggt tgctgaacgg cgttccaatt cgcattgccg ggcgttgacc
      601 ggggttgtga ttgcccctgg ttattgggca gggacggcgg gggcgttggg atcgcctctt
      661 ggctaggctg gatcccctgg ggggctgggc cgggtggtac ggctggggtc ggcgactggg
      721 acgtgggtga aggtgccaga tcttcgtgga gtgccacgac gtcggccagg caccagtgca
      781 ttcgtgctgc ttcgatctgc cgcgcccgtt ccacgtcctc gtccgttcga accacgtgtg
      841 gtgtaaggaa gatcagtaac tcggttcgac tcgcatcgcc caggtcatat cggaagagat
      901 ggcggagaat cggaacgtca cttaggccag gaatccggcg gttggtcatt gaacggctct
      961 tagtgatcaa gccaccgagg atgacggttt gaccatctcc cgcgctgatt gtcgtctgtg
     1021 ctcgggtcgt gttgatccgt ggcgatcgaa tgatctgccc ggcagcggaa attgagatgg
     1081 gaattccttc ggactcggga ccgacatccg atttttctgc gttaatttcc atgacgactt
     1141 ggccatccgg gctaatccgc gggcggacca gaagaatcaa ccccgtgttg gtgtagctca
     1201 cactgttgac ttgagcatat tgagttacgg atgttcccgt gacggtgggt acctgttgac
     1261 cgacttgaat gaatgctgcc tgattatcga gggccatgac ttgggggcgg ctgagcacgt
     1321 cgagacgccg cttctgcctc agggcacgga tcaggacact tactgcgtcg ctggaggccg
     1381 acaagaccat tccaccgaag ccaagagtgg aattgcctcg tcctacgccg aaattagaga
     1441 tggcttgggc ccccacaatg tcggaacctg gtgccgtggt gttatttccc aacgcgcctg
     1501 tactgttgaa cagaaaaccg gggactcctg ctacacttcg atcgaacagg ataggatcct
     1561 ggatacccag ttcaattcca aattcgtcgg tattgctgag ttgaacttca gcaatgagca
     1621 cttggatcaa gaccataggg ggacgttcgt caagttgcgt gatgatctcc acaatctcgt
     1681 cgaagtactc tggggtggca ctcacaatca ggctattact cacggtttcc ggaacaatga
     1741 ctacttcctt gctgaggaat tcaaaggtac taaagacgtt cacgttcaat tggcggactt
     1801 gccgctcatt cgtcaagtaa gagttgatgg cattggctac atcgtttgcc ggggcatttt
     1861 ttagccgaaa gacttgtgtc ttccgcttaa tcacttccga ctcgtccagg cgcaggagga
     1921 tggcttcgac taccgccaaa tcgccggcgg aaccgctagc gatgatgctg ttcgtgcgga
     1981 catcaacagc gaatcgaagg gggatcaggg agctttctcc ctcctgggcg cctgtgcggt
     2041 aggccggttg ggcggcggca ccggctgcgg ccgtaacagc ctgcccaaag agggtttgca
     2101 gcatgtctgc caaggcctgg gcgtcactat tgacgatggt gaagaccttg atttgcgctt
     2161 cggcggaagg caaacggtca agttccgtaa tcagggcctc aattaggggc atgctttctg
     2221 ccggggcagc cacgacaagg gcgttggccc gagtgtcggc ggtgatccgc acgtcggtca
     2281 taattcccga ctcgagcaat tgtttgcctt cggtgtccac ggtgacaaag cgtaaccggg
     2341 tagaacggat ctcctgctgg gcttgcgcgc ccgcctggac ggttaactgc cccgtcgccg
     2401 tttgcctgag tgcaccggct gcctgagccg aaatggcttg ttgtaggatg ggagccaatt
     2461 cgctggctag agagttcttc aacgtgaaga ccttaatctc attgaccgcc tcggctgttg
     2521 gggtatcgag cttggcgatc aactgctcga tctcgagcat gtcacgggga gatgccttca
     2581 cgatgacaga attcgagcga aaatctgagg tgatcaaaat ctttgtcccc aatccacccc
     2641 gattagcata aaaagtggtc aatcgctgtt cgattgtggt tgccgacgca tggcgcaatg
     2701 ggaaaacttt gaattcactt tccggtgcca cgggacgatc gagcttggcg atcagctcaa
     2761 gcacggtgtt tacggcttcc tgacgaccaa caagcaagat agcgttgggt ttcacgaggg
     2821 caatcatcgt gatgctgcct cgccgtgccg cataaacgct ttgatagacc tgtgtgagga
     2881 tcgcgagtag gtcttcacaa tcgacgtgac gcagcaaggc gatgcggatc tcaggctcgg
     2941 tctcgacgct gatttcttcg atctgacgaa taatctgcat gacccgttcc acgtcccgcc
     3001 gattcccacg aaccacaagg acgtccaggc cctccagaaa ctcaatctga accgggccca
     3061 caagtccacc accttcctcg ggtgcggcac ctccaggggc ggcttccccg ggggcgggag
     3121 gttcggccgg aggggcttgc accgcaggtg gctgggtgac gcccggttgc ggagcagacg
     3181 gctgctggcc ttgtggctgt tcttgggtgg tagtaactga cggttgtggc gtcggtggct
     3241 gacttggttc aacctgagtg acaacgaggt cctgaggcaa acggttttcc gcttcctgtt
     3301 gtgtgctatt agccgcctga atagcctgcg ctgcctgatg gacagtgctg gaacgcgcgt
     3361 tagccaaagg aatgagcctc acggtctgcc ctgcgcttga ggccgggcta tctagagcct
     3421 gaatgagctg acgggctggc atcagtgacc acatggggcc ggtcacccga atacgatggt
     3481 ttatacgatc cactacaaga cctagctttt ccccagtgtg aagagccatt tggtactccg
     3541 cccgtgcggc ttgggggaca acattgatcg gttcgagggt ggagcctagc attgactgca
     3601 aggccgcttc gagctgagtt gccttgaggt tccaaagcgg tatctcttca attgtgagcg
     3661 aactgcccat ctgagatgtg tcgcggtcca tctctgctga agcgtaagtc atttgcaccg
     3721 ggaagcttcg tccgctggaa ctcggtccgg tacctgactg ctgggcctgt cccgcgggta
     3781 gattcgccgg cgaaccggaa c
//
LOCUS       DAOPTR010000280         3800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19298_length_3800_cov_3.010948, whole genome shotgun sequence.
ACCESSION   DAOPTR010000280 DAOPTR010000000
VERSION     DAOPTR010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19298_length_3800_cov_3.010948"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /locus_tag="PKI05_06515"
     CDS             <1..1434
                     /locus_tag="PKI05_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOA51891.1"
                     /translation="ELLEVLLAEEKPSEELIWRVAKLATCSLQMTPVFLGSAFKNKGV
                     QPLLDAIVRILPSPLEVEVTAIPPGEDQSPLLLTADPEAPAVAMAFKLMEDPYGALTF
                     LRVYQGTLRKGSSYYNQRTGKRERFGRIVRMHADKREDIEAATAGDIVAVLGIDAISG
                     DTYCESYPMCHLERMHVPEPVIKMSITPVERGSAERLAKALARFRREDPTLQVTIDEE
                     SGETLIAGMGELHLDIYVERLRREYGVDVLVSAPKVNYREAPTQPASFNVRHRKQTGG
                     AGQYAHIVGRMEPLPEDCEETFVFEECIFGGHIPKQFIPAIEKGLRSSLEKGPLAGYP
                     IVRLKVIVEDGSYHEVDSSDLAFQICAQQVMREAFPQTKPALLEPIMKIEIECPQTAQ
                     GNVVGDLISRRGVVLGTTSLGNLVRIEGEVPLAETFGYSTTLRSLTQGQGTFTLEFAC
                     YRQVPPSIQEQIVFQRKRNRMPAGASV"
     gene            complement(1528..2802)
                     /gene="larA"
                     /locus_tag="PKI05_06520"
     CDS             complement(1528..2802)
                     /gene="larA"
                     /locus_tag="PKI05_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HOA51892.1"
                     /translation="MRVTLEYGRTGLNVELPDANVVKVLRYRPAEVLPDPRATIRELL
                     WRPIGTPPLPEIAVGRRSACIAICDITRPVPNEVILSELLPLLEESGIPRERITILIA
                     TGLHRPNLGDGLVEIIGKSIAENYRVENHNGQALSEHEFLGESPRGVPVWIDRRYIQA
                     DLKITIGLIEPHFMAGYSGGRKLICPGLAALETVRAWHSPKFLEHPNARAGVLEGNPV
                     HEENTWIGRRAGCDFIVNVVLDEHRKIVHAVAGDMEEAFLDGVRFVEKVVWDTVPEPV
                     DVVITTAAGYPLDATFYQSIKGMVAAEPIVKPGGTVILAAEISEGIGSPEFRRIFEEN
                     SSLDEFLDRITKGDYFRMDQWQLEEMAKVRRKARIVVVTGGLTSDQLSRLFVESAPTV
                     EDAVADCLRRYGPQATIAVMPKGPYLVADVAR"
     gene            complement(2889..3110)
                     /locus_tag="PKI05_06525"
     CDS             complement(2889..3110)
                     /locus_tag="PKI05_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic protein"
                     /protein_id="HOA51893.1"
                     /translation="MTMDKSLRIRKALVKVRSVLTRAERIKRLQQLEQFGEDRSPLGL
                     PKVRVPKISMKKKKKKAKEEAASGQAAKK"
     gene            complement(3426..>3800)
                     /locus_tag="PKI05_06530"
     CDS             complement(3426..>3800)
                     /locus_tag="PKI05_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51894.1"
                     /translation="AQLGKLVGEELLPDSSLPKTYTGPSRIILPTVRGLLTRGESLRL
                     KVILLGFENPDATLLWRSQGEGDYRTVPLSHVARGVYQTQIPGEQLPEIVEYFVEVQD
                     KTRIMRYPATAPTFGLTTVVAQ"
BASE COUNT          821 a         1063 c         1085 g          831 t
ORIGIN      
        1 gaactattgg aagtccttct ggccgaggaa aagccttccg aggagcttat ctggcgtgtg
       61 gccaagctag caacgtgttc cctccagatg acgccggtgt tcctgggttc ggctttcaag
      121 aacaaaggtg tccaaccttt gctcgacgct attgtgagga ttctgccgtc gccgctggag
      181 gtggaagtca cggccattcc tcccggcgag gaccaatcgc ctttgttgct aacagccgac
      241 ccggaggccc cggccgttgc catggccttc aaattgatgg aggaccctta tggggccttg
      301 acctttttgc gggtctatca gggaacgctt cgcaagggga gttcctatta caaccagcgg
      361 actggtaaac gcgagcggtt cggtcgaatc gtccgcatgc atgccgacaa gcgggaggac
      421 atcgaggcgg ctaccgcagg cgacattgtg gcagttctgg gcatcgatgc gataagcggt
      481 gatacctatt gcgagtcgta tccgatgtgc cacttggagc ggatgcacgt ccccgaaccc
      541 gtcatcaaga tgtccatcac gccggtggaa cgcggctcgg cggaacgcct ggccaaggct
      601 ttggcccgct ttcggcgcga agatccgacg ctccaggtga ccatcgacga ggaaagcggt
      661 gagacgctca ttgccggcat gggcgagttg cacctggata tttacgtgga gcgccttcgc
      721 cgcgaatacg gcgttgacgt gctcgtcagt gccccgaagg tgaattaccg tgaggccccc
      781 acgcaaccgg ccagtttcaa cgtccgacac cgaaagcaaa caggcggtgc tggccagtat
      841 gcgcatattg tcggtcgcat ggaaccgctt cccgaagact gcgaagagac attcgtcttc
      901 gaggaatgca tttttggggg acacatcccc aagcaattca tccctgccat tgaaaagggg
      961 ctgcgaagtt ccctggaaaa aggacctctg gccggttacc ccatcgttcg ccttaaggtc
     1021 atcgtagaag atggctcata ccatgaggtt gatagctctg atctggcgtt tcagatttgt
     1081 gcccagcaag tgatgcgaga ggccttcccg cagaccaaac cggccctttt ggagccgatc
     1141 atgaaaattg agatcgaatg cccgcagacg gcacagggca acgtcgttgg tgacttgatc
     1201 agcaggcgcg gcgttgtcct cggaacgacg tcactgggaa acctggtgcg gattgaaggc
     1261 gaggtgcctc tggccgagac gttcggctac tcgaccacct taagaagcct tacccagggc
     1321 caagggacat tcacgctgga gtttgcctgt taccggcagg taccgccgtc cattcaagag
     1381 cagatcgtct tccagcggaa acggaaccga atgccggcag gggcttcggt ctgaaagatt
     1441 ctcgaagatt ctcgagcgcc gagatggttc cgctgggtgg aacgttcgcc acggtgattt
     1501 caaccggctt ctttggcagc aataaaatca gcgggcgacg tccgccacaa gataaggtcc
     1561 ctttggcatg actgcgatgg tagcttgtgg tccataacgc cggaggcaat ctgcgaccgc
     1621 gtcttcaacc gttggggcac tttccacaaa gagtcgggaa agttgatctg atgtcagccc
     1681 accggtcacg accacgatgc gggccttccg ccgcaccttg gccatctctt ccaactgcca
     1741 ctggtccatg cggaagtaat cacccttggt aatgcggtcg aggaactcgt cgagggaact
     1801 gttttcctca aagatacggc ggaattctgg gctaccgatt ccttccgaaa tttccgccgc
     1861 aaggataacg gtgccgcccg gcttgacgat tggctccgcc gccaccatcc ccttgatcga
     1921 ctggtaaaac gtggcgtcca ggggatagcc tgccgccgtt gtgatgacga cgtccaccgg
     1981 ttccgggacg gtatcccaga cgaccttttc tacaaagcga acaccgtcca gaaaggcctc
     2041 ttccatgtcg ccggcaacgg catggacaat ttttcgatgc tcatccagga ccacgttaac
     2101 gatgaaatca cacccggcgc gacggccaat ccacgtgttt tcctcgtgga cggggtttcc
     2161 ttcgagaacg cctgcccggg cgttagggtg ctccaaaaat ttgggagaat gccaggcgcg
     2221 aacggtttcc aaggcggcca atccgggaca aatcaactta cgcccaccgg agtagcccgc
     2281 cataaagtgc ggctcaatca gtccgattgt gatcttcaaa tctgcctgaa tgtaccggcg
     2341 atcgatccaa acagggacgc cgcggggact ttccccaaga aactcatgtt cagagagcgc
     2401 ctggccattg tgattttcta cccggtagtt ctcagcaatc gacttgccga tgatctcgac
     2461 cagtccgtcc ccgagattcg gtcggtgcaa ccccgtcgca atcagaatcg tgatcctctc
     2521 acggggtatg ccagactcct ccaataaagg cagtagctcg gagaggatca cttcgttggg
     2581 aacagggcgc gtgatgtcac aaatggcaat gcaggcagaa cgtcgcccca cggcgatttc
     2641 cggcaacggc ggagttccga ttggtcgcca caaaagctcc cggatggtag cacgtgggtc
     2701 aggcagcact tctgcgggtc gatagcggag gaccttgacg acgtttgcgt cgggcaattc
     2761 gacgttcaga cccgtccggc catactccaa agtcacgcgc atagacaaac acctcgcgct
     2821 ggtgacgcgt taaaatcgcc ccgcagccga tgaaggacct gacccgggag gccgaaaacg
     2881 gcgacccgtt acttctttgc cgcctgaccg ctagccgcct cttccttagc cttcttcttt
     2941 ttcttcttca tggagatttt tggcacgcgg acctttggca agccaagggg ggagcgatcc
     3001 tcgccgaact gttcaagctg ctgaagacgc ttaatccttt cggcacgggt caaaacgctc
     3061 cgcaccttga ccaaggcctt tcgaatgcgg agacttttgt ccattgtcat gggtttgtac
     3121 tccttatttg ggtatttcca ggctattgtc cgttggcgcc agtacggcaa tattgagtga
     3181 ccaattggcg ctcggtggca ttataatttt ggactgctag cgaactggcg cttatggcgt
     3241 caggtgagcg aaattgccaa aacttcaatt ttataatgtt catccgccga cggcaacgaa
     3301 ggccgaatat ttgtgactgt acgttctgtt aaaatcggcg tgtgggtgtt cccaagaggc
     3361 atcctgtcaa aaaattgctc tgtgggtttg atgcatcgtg cgaatgaaat ggttagaaag
     3421 ggtgcctact gtgccaccac agtggtgagt ccaaatgtcg gcgccgtggc gggataacgc
     3481 ataatccggg ttttgtcctg cacctccaca aagtactcga caatctcagg gagttgctcg
     3541 ccagggattt gcgtttgata aacgcctcgt gctacgtgag agagcggtac tgtgcggtaa
     3601 tcaccctccc cctgggaacg ccacaatagg gtggcatcag gattctcaaa acccaggagg
     3661 atgactttca gccgaagtga ttccccgcgt gtaagtagac cccgaactgt ggggaggatg
     3721 atcctgcttg ggcccgtgta agtcttgggc agactgctat cggggagcaa ttcctcgccg
     3781 accagtttgc caagctgggc
//
LOCUS       DAOPTR010000281         3792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19339_length_3792_cov_3.645973, whole genome shotgun sequence.
ACCESSION   DAOPTR010000281 DAOPTR010000000
VERSION     DAOPTR010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19339_length_3792_cov_3.645973"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1904
                     /gene="speA"
                     /locus_tag="PKI05_06535"
     CDS             <1..1904
                     /gene="speA"
                     /locus_tag="PKI05_06535"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HOA51895.1"
                     /translation="TRRWTVADAEELYEVQSWGKGYFSVNAAGHVCVHPEKDPQRSID
                     LKHLVDRLIVRGIQTPVLIRFGGILKHRLGEIKQAFEEAIREHNYKGRYHLVYPIKVN
                     QQRQVVEEILRYGAAYQFGLEAGSKPELLAVIALADNTTPIVCNGFKDARFIETAMLA
                     QKMGRNIFLVVEKYTDLELILHFAKEVGVRPNIGIRVKLAAMGSGKWQSSGGHRSKFG
                     LTVSEILRALRELKEHDMADCFKLLHFHLGSQITNIRHIKTALTEGARVFADLYKQGA
                     GLQYLDVGGGLGVDYDGSQTTFESSVNYTLQEYANDVVYHIQQVCDDAGVPHPTIFSE
                     SGRAVVAYNSVLVFNVLGVADRGENGIPEQIPEDAEQPLHDLRQTYDNLNVRNLLESF
                     HDAQEAMENAINLFNNGYMSLEQRAFAENLYWAICRKIFRLMQHLDYVPEELEVLDSL
                     LSETYFCNFSLFQSLPDSWAIKQLFPIMPIHRLTEQPTQRGILADITCDSDGKIDRFI
                     DRHDVKRTLPLHVFRGEPYFIGVFLVGAYQEILGDLHNLFGDTNAVHVGLDEDGDVVV
                     ETVINGDTVREVLSYVEFDPNTLVVRLRNSLEEAIRRGRIDDRQAGRLLRFYESGLEG
                     YTYLENMPE"
     gene            1995..2765
                     /locus_tag="PKI05_06540"
     CDS             1995..2765
                     /locus_tag="PKI05_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOA51896.1"
                     /translation="MKDSIFGLANKTALVTGASRGIGRAIALAFAQAGAEVFICSRKA
                     ESIEAAAKAIREQTGARVEWMAADVSQRQAVDQLAAEALRRMGKIDILVNNAGWNIPQ
                     AIDEIQDKDWDYLIELNLTNCMRLTRAIVPDMKKRRWGRVIHISSMMAMGSTPKRNAY
                     SATKAALVGMARASALDLGPWNITVNCIAPGPIATEMPMTLLSKEQQEQMTQRTALGR
                     WGQPEEIAWPTLFLASEAARYITGQVIVVDGGCLARII"
     gene            complement(2871..>3792)
                     /locus_tag="PKI05_06545"
     CDS             complement(2871..>3792)
                     /locus_tag="PKI05_06545"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HOA51897.1"
                     /translation="LGKSYQEEIRNPDLSFADPAEALAKVVPQLKQQADILVLLAYAA
                     RDEAIALVREFPEFAVVAISDGPAEPPGAPQFVPDTQTMLVFVGEKGMAAAVVGIYER
                     NNPPMAYQRVILDSRYPDSPAMKEMMATYQAQLQALGLEGLEIRPAPHPRREVQGEFV
                     GSEKCAACHEESYRVWKRSGHSRAWETLVRLDPPRNFDPECISCHVTGWHPTRYFPYL
                     SGFLSEEKTPHLINVGCESCHGPGGEHVAAEMGSDLERQRRAAEAMIVTKEQAESSEA
                     HSCRNCHDLDNSPDFDFSTYWPLVEHYEKE"
BASE COUNT          827 a         1009 c         1118 g          838 t
ORIGIN      
        1 agacacggcg ctggacggtt gccgatgccg aggaactcta cgaggtccag agctggggca
       61 aggggtattt ctccgtgaac gcggcggggc acgtatgtgt ccatccggag aaggatcccc
      121 agcgatcgat cgatttgaaa catttggtcg atcgcctgat cgttcgtggg atccaaacgc
      181 ccgtgcttat ccgtttcggc ggtatcttga agcaccgttt gggcgagatc aagcaggcct
      241 ttgaggaggc catccgagaa cacaactaca aaggccgcta tcacttggtc tatccaatta
      301 aggtcaatca gcagcggcag gtcgtcgagg agattctccg ttacggggcg gcttatcagt
      361 ttgggcttga agccggcagc aaaccggaac tcttggccgt cattgccctg gccgacaaca
      421 caactcccat cgtttgcaac ggcttcaaag atgcccgttt catcgagaca gccatgctgg
      481 cccaaaagat gggacgcaac atcttcttgg ttgtcgaaaa gtacaccgat ttggagctaa
      541 ttctccattt tgcaaaagag gtgggagttc ggcctaacat cgggattcgt gtcaaactgg
      601 cggccatggg gtccggcaag tggcaatcct caggggggca ccgctcgaag tttgggctta
      661 cggtcagcga aattctccgg gctttgcgcg agctcaaaga gcacgacatg gccgattgtt
      721 tcaagttgtt gcatttccac ctgggcagcc agatcacaaa tattcggcac atcaagacgg
      781 ccctcacgga aggtgccagg gtcttcgcgg acctctacaa gcaaggtgct gggttgcagt
      841 acctcgatgt cggtggcggc cttggcgtcg attacgacgg ctcgcaaacg acgttcgaat
      901 ccagcgttaa ctacaccttg caggaatatg ccaacgacgt tgtgtatcac attcagcagg
      961 tctgcgatga tgcaggcgtg ccccatccga cgattttttc cgaaagtggt cgggccgttg
     1021 tggcctacaa cagtgtgctg gtgttcaatg tgcttggcgt tgccgaccgg ggtgaaaacg
     1081 gcatcccaga gcagattccc gaggatgccg aacagcctct ccatgatctc cgacaaactt
     1141 acgacaactt gaatgtacga aatctcttgg agagcttcca cgacgcccag gaggccatgg
     1201 aaaatgcgat caatctgttc aacaacggtt acatgtcgtt ggagcaacgg gcatttgcag
     1261 agaatcttta ctgggcaatt tgccgcaaga tcttccgcct catgcaacat ttggattatg
     1321 tcccggagga actcgaagtt ctcgattccc tattgagtga gacatatttc tgtaactttt
     1381 cactgtttca atcgttgccc gatagttggg cgattaagca gctctttccg atcatgccta
     1441 tccatcgtct cacagagcag cccacacagc gggggatttt ggccgatatt acgtgcgact
     1501 ccgatgggaa gattgaccgc ttcattgatc gccacgatgt gaagcggact ttgcccctcc
     1561 atgtatttcg cggtgagccc tacttcattg gcgtatttct ggtaggggct taccaggaaa
     1621 tcctcggcga ccttcacaat ctgtttgggg atacgaatgc ggttcacgtg ggactggacg
     1681 aagacggcga cgtggtggtg gagaccgtca tcaacggtga taccgtgcgc gaggtcctat
     1741 cctacgtgga gttcgatccc aacacgttgg tcgtgcgttt gcgaaatagc cttgaagagg
     1801 ccattcggcg gggacgcatc gacgatcgtc aggccgggcg gctgctccgc ttctatgagt
     1861 cggggctcga aggctacacg tacttggaga acatgcccga gtgatggcgg cgccacgtcg
     1921 aaaccgcgtt acgcagcgaa gattttgagc gaatcctagg caggcttcat ttgattgcac
     1981 gtgacggtga cagcatgaaa gatagcatct ttggcttggc gaataagacg gcacttgtga
     2041 cgggagcaag tcgagggatt gggcgtgcca ttgccttggc ttttgctcag gccggggcgg
     2101 aagtcttcat ttgcagtcgc aaggcggaga gcattgaggc cgcggcaaag gcgattcgtg
     2161 agcaaacggg ggctcgggtg gagtggatgg ccgccgacgt ttcgcagcgc caggccgtgg
     2221 accaactggc cgccgaggcc cttcggagga tgggcaaaat tgacattctg gtcaacaatg
     2281 cgggctggaa tattccccag gccattgacg aaatccaaga caaggactgg gattacctga
     2341 tcgaactgaa tctgaccaat tgcatgcggc tgacgcgggc cattgttccc gacatgaaaa
     2401 agcgccgctg gggtcgcgtg atccatattt cgtccatgat ggcgatggga agcacgccca
     2461 aacgcaatgc gtattctgcc acgaaggcgg cgttggtggg catggctcgg gcaagtgccc
     2521 ttgatcttgg cccgtggaat attacggtca actgtatcgc tcccggtccc attgccacgg
     2581 aaatgccaat gacgctcctc agcaaggagc agcaggagca gatgacccag cgaactgcct
     2641 tgggccgttg gggacagccc gaggagatcg cctggccgac gcttttcctg gcaagtgaag
     2701 cggcacgcta cattactggt caggtgattg ttgtcgatgg cggctgcttg gcacgaatca
     2761 tctgatcatc taaaaaatcc ataaaggtcc ggcgacaggc taccggttag gccagaatga
     2821 accagtgcca gaccgccaca tttccacaag gctacatcac ctcgacgccg tcactctttt
     2881 tcgtagtgtt cgactagggg ccagtatgtg gaaaagtcaa aatcggggct gttatcgagg
     2941 tcatgacagt ttcggcacga gtgggcctcc gagctttcgg cttgttcctt ggtaacgatc
     3001 attgcctcgg ctgcccgtcg ttgccgctcg aggtcacttc ccatctcggc ggccacgtgt
     3061 tctccgcctg gcccatggca cgattcacaa cccacgttga tcaggtgtgg tgtcttttcc
     3121 tcggaaagga aacccgaaag gtaagggaag taccgcgtgg gatgccagcc cgtcacgtga
     3181 cagctaatgc attcgggatc gaagttgcgt ggcggatcaa gtctcacaag cgtttcccat
     3241 gcccgagagt ggcctgaccg tttccaaacg cgataggatt cctcgtgaca tgcagcacac
     3301 ttttcagacc cgacgaactc gccttgcact tcccgtcgcg gatgcggagc cggacgtatc
     3361 tcaagtccct ccagcccaag ggcctgaagt tgtgcctggt aggtggccat catctctttc
     3421 atggcgggcg agtcgggata acgagagtcg agaatgaccc gctggtaggc catcggcggg
     3481 ttgttacgct cgtaaatgcc aacgacggcc gccgccatcc ccttctcacc gacgaagacg
     3541 agcatcgttt gggtatcggg aacaaattgc ggggcacccg gcggttctgc cggaccatca
     3601 gaaatggcta ccacggcgaa ctcgggaaat tcgcggacta gggcgatggc ctcatcccga
     3661 gccgcatatg ccaacaggac aaggatgtcg gcctgttgct ttaactgggg gacaaccttg
     3721 gcgagggcct cggcaggatc ggcaaaggat aggtcgggat tgcgaatctc ctcttggtag
     3781 ctctttccga gg
//
LOCUS       DAOPTR010000282         3784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19389_length_3784_cov_3.560204, whole genome shotgun sequence.
ACCESSION   DAOPTR010000282 DAOPTR010000000
VERSION     DAOPTR010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19389_length_3784_cov_3.560204"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(327..965)
                     /locus_tag="PKI05_06550"
     CDS             complement(327..965)
                     /locus_tag="PKI05_06550"
                     /inference="COORDINATES: protein motif:HMM:NF024879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HOA51898.1"
                     /translation="MLPSIRFTFWLWGIGLIAAFCSGCTSTHADRGALFGGLLGAGTG
                     AIVGNAAGNTLAGTAIGAGVGALAGSAIGDSLDEMEARNRALIAQQMGRDIPRGQVTF
                     EDVIAMTRANVDEELIINHIRINGMSRIPSSDDIIRLQQEGVSVKVIKAMQEAPIRRS
                     EPSDRPIIVREPAPPPVIIYERDPWWWGPPRWHRPYYYHPRPGVSWGITFHN"
     gene            complement(1263..2615)
                     /locus_tag="PKI05_06555"
     CDS             complement(1263..2615)
                     /locus_tag="PKI05_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA51899.1"
                     /translation="MKDLSLHGNRRQFLTGTSALALGAAVAGQVPAVHAGEDNTIRLA
                     LIGCGGRGAGAVGDALSTRDMGPIKLWAMADIDQERMEACYKALREKFGDDIDVSPER
                     RFTGFDGYKKAIDLLRPGDIAMCTTRAYIRPLHVEYAVSRGINVFMEKPFAPDPVGLR
                     RLLKAGEEAEKKGVKIAAGLQCRHSPARAALIEKINSGELGELICVRANRLGGRSWLG
                     NVGPKANSFLEQLKFGRIHLFWVGSGHFADYLIHQIDECCWLMGSWPVSCHGMGGREV
                     GSTDRGQNLDIYSLEYTFADGRKAFCGFRRALNAHNEFATFVHCSKKAGQFSGDVHAA
                     TVHIFKDQRIDKNNIEWSPTPDQYSPWVYEWRDFITSIRTDRPHNECKRAVYSDFATL
                     MGRAAAHYNRIVTWDEVFNSQFQFCNNLDQLNYDSPPPVTADENGYFPAPVAGQWTEL
                     "
     gene            complement(3377..3574)
                     /locus_tag="PKI05_06560"
     CDS             complement(3377..3574)
                     /locus_tag="PKI05_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51900.1"
                     /translation="MTLLDWLRKLGILRYGAEATVFHNAKERPLSFQEDDVLNSQKDV
                     VRFGKKDELTNKPEGRKAKTT"
BASE COUNT          841 a         1144 c          966 g          833 t
ORIGIN      
        1 gggcatgttt caaccgtttt ctaaaatctc ctacccccct ttagactggg gatcgcgtga
       61 aacggatggc ttttcctcgg gctgcgcacc cggcttctcc ccgggtaact ctatccggcg
      121 cgtgaacaat cttttaactt accggcaagc acggcaatgt caagggactc cgcctcccgc
      181 caagatcttt ggcactgacc ggtgtacttt ggtccaattc aagccggatt gacactcccg
      241 ccttgccggg atcggcggca agccggattt tctgcatcct tcctcgaacg gccaccactc
      301 ggaaaagctc tgggcgactc gatcgattaa ttatggaaag tgatccccca actcaccccg
      361 ggtcgggggt ggtagtagta ggggcgatgc caacggggcg gtccccacca ccaggggtca
      421 cgttcataga tgattaccgg cggtggggcc ggctctcgaa caataatcgg acggtccgac
      481 ggctcagagc ggcgtatggg ggcctcctgc atggctttga tgaccttgac gctgaccccc
      541 tcctgttgca ggcgaataat gtcatccgaa gaggggattc ggctcatccc gttgatccga
      601 atatggttga tgatcaactc ttcgtccacg tttgcccgcg tcatggcaat cacgtcttca
      661 aaggtgacct gccctctggg gatatcacgg cccatttgtt gggcgatcaa ggcccgattg
      721 cgagcctcca tttcgtcaag gctgtcgcca atggccgagc cagcgagggc ccccactccg
      781 gcgccaatcg ctgtaccggc gagagtgttt ccggccgcgt ttccgacaat ggcgcccgtc
      841 cctgcaccca gtagcccacc aaaaagggca cctcgatctg cgtgcgtgct ggtgcaaccc
      901 gagcagaagg cggcaattaa cccaattccc cagagccaaa acgtgaacct aatggatggc
      961 aacattatca tccgtgaact ccttggctgg gcacgtcctc cctaaccgcc gaggttttga
     1021 aataagccgg gaaattgaca ttctctatcc cataccttat cggctacttt tttcgcccga
     1081 ccaaatccat tcgatgaaaa tgtcaatttg cgcagaataa aaagccatcg cccaagcgtc
     1141 aagcgaaaat gggcgacgtt gtcacaacct aggcaagggc agcacaacag gcgggccgca
     1201 caacttgcga tgcttcagac ccgccggatg cgattatcag aagccgtttt cccaccaatc
     1261 aatcaaagct ctgtccactg gcccgctacc ggagccggga agtacccgtt ttcgtcggca
     1321 gtgacagggg gtggcgagtc gtaattcaac tgatccaagt tattgcaaaa ctggaactga
     1381 gagttaaaga cctcgtccca ggtcacgatg cggttgtagt gagcagccgc ccggcccatg
     1441 agcgtggcaa agtccgaata aaccgcccgc ttgcactcat tgtgtgggcg atccgtacgg
     1501 atacttgtga tgaagtcacg ccattcatag acccaggggc tatattggtc gggcgtcggg
     1561 gaccattcga tattgttctt gtcgatccgc tgatccttga agatatgcac cgttgccgcg
     1621 tgtacatctc ccgagaattg acccgccttt ttcgagcaat ggacaaacgt ggcaaactca
     1681 ttatgagcgt tcaaagcacg ccggaaaccg caaaaggcct tgcgcccatc ggcgaacgtg
     1741 tactccagcg aatagatatc caagttttgg ccacggtcgg tactccccac ctcgcgcccg
     1801 cccatcccgt ggcaagagac cggccaggaa cccatgagcc agcagcactc gtcgatctga
     1861 tggattaagt aatcggcgaa atgccccgac ccgacccaaa aaagatgaat ccgtccgaat
     1921 ttgagctgtt cgagaaaact attggccttg ggaccaacgt tgcccaacca ggaccgccct
     1981 cccagccgat tggcgcggac acagatcaat tcgcctaact cgcccgagtt aatcttttca
     2041 atcaaagccg ctcgggccgg tgaatggcgg cactgcaaac cggccgcgat cttgacgcct
     2101 tttttctcag cttcttcccc agccttaagc aaccgtcgaa gtccgaccgg gtcgggtgcg
     2161 aatggctttt ccataaacac gttaataccc cgcgacacgg catactccac atgaagtggc
     2221 cggatgtatg cccgcgtcgt acacatagcg atgtcgcccg gccggagaag gtcaatcgcc
     2281 ttcttgtacc catcgaatcc cgtaaaacgg cgctccggcg agacgtcgat atcgtcacca
     2341 aacttctcgc gcaaggcttt gtaacatgct tccatgcgct cctggtcgat gtcggccatc
     2401 gcccaaagct taatcggtcc catgtcgcgg gtacttaaag cgtcccccac cgctcctgca
     2461 ccacggcccc cacaaccaat aagggccaat cggatggtat tgtcttcccc cgcgtgaaca
     2521 gcaggcacct ggcccgcaac tgcagccccc aaggctaaag cacttgtgcc tgtcaaaaac
     2581 tgtcgtcggt tgccgtgaag ggataggtct ttcatcgcta aaacctttct gcacaaatca
     2641 cgcttgttaa cagggtcctc aatccacggg tacccgttgg atcccataac aacgggtcaa
     2701 cgtccaccgt taggtaacgc ttgtggcccc tttctagact atcccactag ctcacgctaa
     2761 gaacctcatt gcgcaatcgc aatcctcgcg cacaataacg gcgcagcagc ttggcgtata
     2821 gccatattgt cagactgccc cttgtccgtc aagcatctgg ggctattgcg gagtggaagg
     2881 tgtacacttc catggaaaaa acgagatgat cgcctctgtc aggttcgcag aattttgccg
     2941 gatgatcgaa gtcgctaaga tgagtggaga ttcggcccgc aatttactgc tgctcttgat
     3001 cgtgttcttt cagaacactc ccacgctacg tgaggggccc cagccaactc ggttttcacg
     3061 ctttctccgt tcagcggccc gatgctaagt ggcggtgacg ttagcgtttc tcctccgttt
     3121 cgcatggatc cacgtcccgc tcatgcaatc gttacttcgc acgaagcctg gggacgggaa
     3181 aacgtggtgg aactttgatc gggcggcctc cacagccctc gtgctgaggg aaattcgcag
     3241 gtcgagcccg gctattcacc gacgcgcgaa aagctctgac agccaaacgc aagtcatcgc
     3301 cgccataccg ctcacaaaaa gccgacagaa gttggcagca acgtcggatt taacaaaatg
     3361 ttcaattcgc gtgttgttag gtcgttttgg cttttcggcc ttccggcttg ttagtcagct
     3421 catccttttt gccgaaacgc acgacgtcct tctgcgagtt gagaacgtcg tcttcttgaa
     3481 aacttaaagg tctttctttt gcgttatgaa acacggtggc ttcggcacca tatcgcaaaa
     3541 taccgagctt acgcaaccaa tcgagtaacg tcattttcgt tctcctggaa caagtctgta
     3601 acgttttctc cgtgtgctcc cgtgagactg ccgctcgccg agggttaaga gcctatctcg
     3661 aaaggtcccc ctctccctcc gggagaacgt tcgggtgagg gcaacggaac gtcggaattg
     3721 atcgaggaca ccggcagtga ttcgagccac cctcaccccc gacccctctc ccgccgaacg
     3781 ggcg
//
LOCUS       DAOPTR010000283         3779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19424_length_3779_cov_3.885875, whole genome shotgun sequence.
ACCESSION   DAOPTR010000283 DAOPTR010000000
VERSION     DAOPTR010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19424_length_3779_cov_3.885875"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..1536
                     /locus_tag="PKI05_06565"
     CDS             169..1536
                     /locus_tag="PKI05_06565"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOA51901.1"
                     /translation="MRGLVYVYLMTYLGAVVSLFYPLVGLFIYIHYAILKPESLWYWS
                     VGAQGPGRYSFIVGVGMLVGWAIRGFGDWRLGRSTAVAGCLVGFLAWGSVGYFFCWEP
                     ALAKVFIISLLKIVLPFLLGISLLQTTNHVRWLAWVIVITQGYLALDFNRSYYQGINR
                     IVLEAYGGFDNNCIAAAMVVGTLLAFFLGIAESRWELKTLAFAACGLMAHTIMFSFSR
                     GGLLGLIAGGTAAFLLIPKTFKTTLIYGLAIILALRLAGPEVRQRFMTTFVDPEQRDR
                     STRSRVEQWKACLQTIRDNPFMGVGVNHWPLQSQRLGLPKMHAHSLWLETGAETGIPG
                     LLLLSGFYGLTIFRLWTLIRQCPHLDLWSQNMARGVVAALIGFAVSVSFISVMWLEIP
                     YYTALLGAALLRVHTPTWMGRHEVLAEPTLEAVAAPLDSPADASFPAFRDHSGYGSGS
                     RPRFA"
     gene            1589..2530
                     /locus_tag="PKI05_06570"
     CDS             1589..2530
                     /locus_tag="PKI05_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA51902.1"
                     /translation="MEQRQADSPQRSPLVSDELGWEWSSSDTRRLGPMFREMVQELWQ
                     YRELLGQMVLRDIRIRYKQAVMGFGWALLMPLLIVGAGCLVKIALARASGDGVDLPRV
                     AGMSVKALGWAFFAGALGFAANSLVSNMTLVTKVYFPRELFPLSAVLTQLFDTLVGSI
                     FLSVLLVVFAHIGASWNLLWVPVLVFILAAQTAAVALLFSCANVFFRDVKYIVQVLLT
                     FGIFFTPVFYEPELFGATGSQLMMLNPLSPVLEGLRLAVVEQHNLLRPLAADLPGAAF
                     WIWHPGYLLYAGLVAVVGFFVSWLIFHRSEFVFAEYI"
     gene            2594..>3779
                     /locus_tag="PKI05_06575"
     CDS             2594..>3779
                     /locus_tag="PKI05_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA51903.1"
                     /translation="MSSDVLVEVQHLSKKFRRGESYDSLRDLIPGLVRRLGFSRGQSQ
                     PTDRWFWALRDVSFQVRRGEVLGIIGANGAGKSTLLKILARILRPTEGYVRVNGRLRA
                     LIEVAAGFHPDLTGRENIFLNGAILGMRMAEIKARFDEIVEFAGIGPFLDTPVKRYSS
                     GMFARLGFAVAAHLEPDILLVDEVLAVGDAAFQAKCLGKMQHVAGQGRTVIFVSHNMR
                     AVMQLCDRVILLEHGRITQEGPAEQVVDAYLRQSLAQYQSWVCELPGLPDAPFEVHRL
                     RLVTEDGRPAPVVPRSQPIVIEITGEVRAADRRLIVAIDIKTIDDLTLFRTHSFEQDQ
                     SYAILGRPGPFCLSCRIPAELLPAGKYNVGLVLAERGVREFFHHRQVLQFEVYQDRPI
                     RGS"
BASE COUNT          658 a          989 c         1138 g          994 t
ORIGIN      
        1 atgggcggac ttatcaacag gggaaaaaga tcggttttcg ggatgccctc caggccgtgt
       61 ggtgcgtcct tcgctactgg tggagcgatt gagacaattc ttcaccggac gaagcaagca
      121 taaaggatgc gacttgcgaa cgaacttgac atcttctgag gcctagcgat gcgcggtttg
      181 gtgtacgtct atttgatgac ctatctgggg gccgtcgtat cgctttttta tcccttggtg
      241 ggccttttta tctacatcca ctatgcgatt ttgaagccag agtctctttg gtactggtca
      301 gttggggcac aaggaccggg gcgctatagc tttattgtgg gggtggggat gctggtgggt
      361 tgggccatcc gtggttttgg ggattggcgg ttgggacgat ccacggccgt ggcagggtgc
      421 ctggtgggat ttttggcctg gggctctgtg ggatatttct tctgctggga accggcactt
      481 gccaaggtct ttatcatttc gctactcaaa atcgttctac cattcctgct ggggatcagt
      541 ttgcttcaga caaccaacca tgttcgttgg ctggcatggg ttattgtgat cacacagggt
      601 tatttggccc tggattttaa tcgcagctac taccaaggga tcaaccggat tgtgctggag
      661 gcttatggcg ggtttgacaa caattgcatc gcggcagcca tggtggtcgg gacgttgctg
      721 gcgtttttcc tcggtatagc ggagtcccgc tgggaactga agaccctggc ctttgcggcc
      781 tgcggcttga tggcccatac cattatgttt tccttttccc gcgggggact cctgggactg
      841 atcgcagggg gaacggccgc cttcctattg attcccaaga cgtttaagac gaccctcatc
      901 tatggattgg caattatttt ggctcttcgc ttggccgggc cagaggtccg tcagcgattc
      961 atgaccacct ttgtggatcc ggagcagcgt gaccggtcca cgcgaagccg ggtcgaacaa
     1021 tggaaggcgt gtctccagac cattcgggac aaccctttca tgggcgtcgg ggtcaatcac
     1081 tggcctctgc aatcgcagcg cttggggtta cccaagatgc acgcccattc cctttggttg
     1141 gagacgggcg cggagacggg gattccaggc ctgctcttgt taagcggatt ctatggcttg
     1201 accatctttc gcctttggac actgatccga cagtgtcccc atttggacct ttggtcccag
     1261 aacatggcac ggggggtcgt tgccgcctta attggctttg ccgtgtcggt gagctttatc
     1321 agcgtcatgt ggctggaaat cccctattat actgccctat tgggcgcggc cttgctacga
     1381 gtccacacac ccacgtggat gggccgtcat gaggttttgg cagagccaac cctggaagct
     1441 gtggcagccc cgcttgatag ccccgccgac gcctcgttcc ctgccttccg tgaccattcc
     1501 ggttatggtt ccggatcaag gccgcgattt gcctgaacaa gaaagcctcc catagtcctc
     1561 gatgtcctgt aacccagcag accctcccat ggaacaacgg caagctgata gtccccagag
     1621 atcaccgctg gtatccgacg aattgggttg ggagtggagt tcgtcggata cgcgtcgtct
     1681 cgggccgatg ttccgcgaga tggttcagga attatggcag taccgggaac ttctcggaca
     1741 aatggtcctg agggatattc gtatccgtta taaacaggcg gtgatggggt ttggctgggc
     1801 gttactgatg cccctgttga ttgtcggtgc aggttgtctg gtgaagattg ccctcgcccg
     1861 ggccagtggc gatggggttg atttgccgcg tgtggcgggg atgtccgtca aggcccttgg
     1921 ctgggcgttt tttgcgggtg ccctgggatt tgcggccaac agtttggtct cgaacatgac
     1981 gcttgtgacc aaggtctatt ttccccggga attgtttccc ttgtccgcgg tcctcacgca
     2041 actttttgat accctcgtgg gcagtatctt cttgtcggtc ctgctggtgg tgtttgccca
     2101 cattggagcc agttggaatc tgctttgggt tcccgtgctt gtgttcatat tggcggccca
     2161 aacggcggcc gtcgcgcttt tgtttagttg tgccaacgtg ttctttcggg atgtgaagta
     2221 catcgtgcag gtgcttttga cttttgggat cttcttcacg ccagtttttt acgaaccgga
     2281 actattcggt gcgacgggca gccaactgat gatgctcaat cccctttcgc ccgttttgga
     2341 aggccttcgc cttgccgtgg tagaacagca caatctgctg cgaccgcttg ctgcggactt
     2401 gcccggtgcc gcattttgga tttggcatcc ggggtatctt ctctatgcgg gccttgtggc
     2461 tgttgtgggc ttttttgttt cctggttgat tttccaccgg agtgagttcg tctttgcgga
     2521 atatatttga gatggcctgc cgttgcccgg ccgttatcgt tggtccggcc cgaagagtca
     2581 tccatggcta gcaatgtcat ctgacgtctt ggtagaagtt cagcatctaa gcaagaagtt
     2641 tcgccgcggc gaatcctatg acagcctccg cgacctgatt ccgggattgg tccgtcggtt
     2701 agggttctcc cgtggtcagt ctcagcccac tgatcgttgg ttctgggccc tccgcgatgt
     2761 ctcctttcag gtccggcgag gcgaagtgtt aggcattatt ggggcaaatg gggcgggaaa
     2821 aagcaccctc ctgaagattc ttgcccgcat ccttcggccg accgaaggat atgttcgcgt
     2881 gaacggtcgg ctccgagcct tgatcgaagt tgcggcggga tttcatccag atttgacggg
     2941 acgcgagaat atcttcctca acggggcaat ccttggcatg cgtatggccg agatcaaggc
     3001 gagattcgat gaaatcgtgg aatttgccgg gattggacca tttctcgaca cgcccgtaaa
     3061 gcgctacagc tccgggatgt tcgcccgatt aggctttgcc gtggccgccc atcttgagcc
     3121 ggatattcta ctggtcgatg aggtgctggc ggtgggcgac gcggcctttc aggcaaagtg
     3181 cctggggaag atgcaacacg tcgcgggcca ggggcggaca gtcatttttg tcagtcacaa
     3241 catgcgggcg gtcatgcagt tgtgtgatcg cgtcatcctc ttggagcacg gacggatcac
     3301 ccaggaaggg cctgccgaac aggtcgtgga tgcctatctc cgccagagcc tggcccagta
     3361 tcaatcgtgg gtgtgtgaac ttccgggcct gcccgacgcc ccgttcgagg tccatcggct
     3421 tcgattggtc accgaggatg gtcggcccgc cccggttgtt cctcgcagtc agccgatcgt
     3481 catcgagata acaggagagg tccgtgccgc ggatcgccgc ttaatcgttg ctatcgacat
     3541 taagaccatc gacgatttga cactttttcg gacgcatagt tttgaacaag atcaatccta
     3601 cgcaatcttg ggccggcccg gaccgttctg cctgtcctgt cggattcctg cggagctgtt
     3661 gcccgcggga aaatacaacg tcggtctggt ccttgccgaa aggggagtcc gtgagttctt
     3721 tcaccaccgg caagtcctcc agttcgaagt gtatcaggat cgccccatcc ggggatccg
//
LOCUS       DAOPTR010000284         3778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19432_length_3778_cov_4.033038, whole genome shotgun sequence.
ACCESSION   DAOPTR010000284 DAOPTR010000000
VERSION     DAOPTR010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19432_length_3778_cov_4.033038"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /gene="rpsM"
                     /locus_tag="PKI05_06580"
     CDS             <1..213
                     /gene="rpsM"
                     /locus_tag="PKI05_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOA51904.1"
                     /translation="TLLDKDYTVEGQLRRQIAQNINRLKDINCYRGIRHRRGLPVRGQ
                     RTRTNARTRKGPRKTVAGKKGVKDLR"
     gene            279..665
                     /gene="rpsK"
                     /locus_tag="PKI05_06585"
     CDS             279..665
                     /gene="rpsK"
                     /locus_tag="PKI05_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOA51905.1"
                     /translation="MAKIGPKRKKIRRHVTQAIVHIKTTFNNTMVTVTDLKGETLCWA
                     SAGTCGFKGSRKSTPYAGQMAAQQAAEKAIKMGVKEVEVKVKGPGAGRESAITALQTA
                     GLVVKSIEDVTPLPHNGCRPKKRRRV"
     gene            751..1377
                     /gene="rpsD"
                     /locus_tag="PKI05_06590"
     CDS             751..1377
                     /gene="rpsD"
                     /locus_tag="PKI05_06590"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOA51906.1"
                     /translation="MGRYTGPVCRLCRREGMKLFLKGTRCDTDKCAFERHKATPGMQG
                     VLRGKPTDYALHLREKQKLKRFYGIYERQFRRYFEMATRSPTNTGEVLMSLLERRLDN
                     IVYRLGFALSRAQARQLVAHGHILVNGRKVTIPSYLVSPGDVVSVKNRPRSLDAVQRN
                     LAERQPMVPDFLNLITGPVPEGHVLRNPTLEDVSLPIQPQLVIEFCSR"
     gene            1424..2416
                     /locus_tag="PKI05_06595"
     CDS             1424..2416
                     /locus_tag="PKI05_06595"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOA51907.1"
                     /translation="MRVQWRGLELPSQVVCDRSTLTDRYGKFIAEPFERGFGVTIGNS
                     LRRILLSSLEGSAVTKVKIQGALHEITSLPGVVEDVTDIILNVKALVVRNHSSQPRTL
                     RINRCTKGVVTAADIEPDDQVTIINKDRVIATLTDDVPFVMEMVVENGRGYVPASEHT
                     PQISEIGVIPVDAMFSPVVKVRYEVEETRVGQKTNYDKLTMEIWTDGSITPEMALVEA
                     AKILRKHLNPFVQYTELGPKVFADSRSVLSQDSTLDSKLSVSLADLKFSTRTLTSLES
                     AGIHTVRDLVAMTEQQLLDLRNFGETSLAEVKDRLATLGLRLGMRTSPSPGGVL"
     gene            2497..3159
                     /locus_tag="PKI05_06600"
     CDS             2497..3159
                     /locus_tag="PKI05_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L17 family ribosomal protein"
                     /protein_id="HOA51908.1"
                     /translation="MRHRKKGRYLGRSPSHRRALLRNLASSLFLTEGDFLFEHEEPYV
                     KGRIITTLAKAKEVRPLVERCITIAKRVLPLLEEAKRMEPTAPRNSEAWRRWRQSDAW
                     YEWNRKMAPVVAARRRILRMLGNNKEAVRVLFEKIAPRFVDRPGGYTRIVRLAKRRVG
                     DAGVKAFIEFVGQNDRVKQRKTIRPEFSVETPKPSEPAPVKEATIDTPEAANVSQENP
                     SN"
     gene            3545..>3778
                     /locus_tag="PKI05_06605"
     CDS             3545..>3778
                     /locus_tag="PKI05_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51909.1"
                     /translation="MTTRVAAHFERMCALLVILGMASGCTHLRMPASSPLVPAQMSSD
                     SVALDIIFAHVPPSDDVLNQIWTELDEQFLPQET"
BASE COUNT          891 a         1017 c         1083 g          787 t
ORIGIN      
        1 acgttgctgg acaaagacta caccgttgag ggccagttgc ggcgtcagat cgcccaaaat
       61 atcaatcggc tcaaagacat caactgctat cgtgggattc ggcaccgtcg tggccttcct
      121 gttcgggggc agcgaacacg gacaaacgcc cgaacacgaa aaggcccgcg aaagacggtc
      181 gctggcaaga agggcgtgaa agacctgcgg taagctggat ttcgtcgcga tatttatcag
      241 ggttataagg gtcggaaaag actgcgaggt tttggaaagt ggctaagatc ggtccaaagc
      301 gaaaaaaaat ccgaaggcac gtcacccagg ccatcgtcca catcaagaca accttcaaca
      361 acacgatggt cactgttacc gacctgaagg gtgagacgct gtgctgggcg agcgcgggaa
      421 cgtgtggctt caagggaagc cgcaaaagta ccccgtacgc cgggcaaatg gctgctcagc
      481 aggcggccga gaaggccatc aaaatggggg tcaaagaggt agaagtcaag gtgaaaggac
      541 caggcgctgg tcgagaaagc gccattacgg ccctccagac ggcgggtctt gttgtcaagt
      601 cgattgagga tgtcaccccg ctgccgcata acgggtgccg tcccaagaag cggcgtcggg
      661 tgtgatggga aacacgctga aaaatgatgg gttgaggttg tctaccaact gagtcagctt
      721 tcatcgtcaa gacaaggact gcgttaaaac atgggtcgat atactggacc ggtttgccga
      781 ctctgtcgcc gcgagggaat gaagttgttc ctcaagggca ctcgctgcga tacggacaag
      841 tgtgccttcg aaaggcataa ggccacaccc gggatgcagg gtgtcctgcg agggaaaccc
      901 accgattacg ccctccacct gcgagaaaag caaaagctca agaggttcta cggcatctat
      961 gagcggcaat tccggcgtta tttcgagatg gcaactcggt cgccgactaa caccggcgag
     1021 gtgctcatgt cgctgctgga gcggcgtctg gacaacatcg tttaccgttt gggattcgct
     1081 ttgtcacggg cacaggcccg gcaattggtg gcgcacggcc acatcttagt caacggccgc
     1141 aaggtcacca ttcccagtta cttggtgagc cccggcgatg tcgttagcgt caagaatcgg
     1201 ccgcggagtt tggatgcggt tcagaggaat ctggccgaga gacaacccat ggtgcccgat
     1261 ttcctgaact tgattactgg cccggttccg gaggggcatg tgcttcggaa cccaacgttg
     1321 gaggatgtgt cactcccgat tcaacctcaa cttgtgatcg agttctgctc gcggtaagaa
     1381 agacaaaggg taacccaagg tttaagtcta ccggaggcct tccatgcggg tacagtggcg
     1441 cgggctcgag ttgccgagcc aggtggtttg cgaccgatcc actctcaccg accgatacgg
     1501 aaagtttatt gccgaaccat ttgaacgcgg cttcggcgta accatcggta atagcttgcg
     1561 tcgcatcttg ctttccagtt tggaaggcag cgccgtgacc aaggtgaaga tccagggggc
     1621 cttgcacgaa atcacgtccc ttcccggggt cgtggaggat gtgaccgata tcatcctgaa
     1681 cgtcaaggcc ctggtggtgc gaaaccacag ttcccagcca aggacactgc gtattaaccg
     1741 gtgcaccaag ggggtggtca ctgccgccga tatcgaacct gacgaccagg taacaatcat
     1801 caataaggac cgcgtcatcg caaccctgac cgatgacgta cctttcgtga tggaaatggt
     1861 ggtagagaat ggtcgtgggt atgtaccggc cagtgaacac acaccccaga tttccgagat
     1921 cggtgtgatt ccggtcgatg ccatgttcag ccccgtggtc aaagttcggt acgaggtcga
     1981 agaaacccgt gtcggacaga agaccaatta cgacaaattg acgatggaaa tttggacgga
     2041 tgggtcaatc acccccgaga tggccctcgt agaggccgcc aagatcctcc gtaagcacct
     2101 caatccgttt gtccagtaca ccgagcttgg tcccaaggtc ttcgccgaca gtcgatccgt
     2161 cctgagccag gattcaacgc tcgacagcaa actgagtgtc tccctggccg atttgaagtt
     2221 ttccacaaga acgctgacga gcctggaaag tgccggtatc cataccgtgc gcgacctggt
     2281 ggcgatgacc gaacagcaac tcctggattt gcggaatttc ggggagacgt cgctggccga
     2341 agttaaggat cgcctggcca cattggggct gcgattgggg atgcgaacat cgccatctcc
     2401 gggtggcgtt ctctaatgtg tcgaggattc aacctgtcgt actttgaaaa tagagagatt
     2461 agttttgcga aataaccgtc cggttaggat gtaaccatgc ggcatcgaaa aaaaggtcga
     2521 tatttaggcc gaagtccaag tcaccgtcgt gccctgctgc gaaatttggc ttcctcatta
     2581 tttctgacgg aaggagattt cctctttgaa catgaggaac cttatgtaaa aggcaggatt
     2641 atcaccacct tggctaaagc caaggaggtc aggcccctgg tggagcggtg catcacgatt
     2701 gccaagcgag tcctcccgct tttggaagag gccaaacgca tggagccgac tgcccctcgt
     2761 aactccgagg cttggcggcg gtggcgacaa agcgacgcct ggtatgaatg gaaccgcaag
     2821 atggcgccgg tcgtggctgc gcgacgccgg atcttgcgga tgttgggaaa caacaaggaa
     2881 gccgttcggg tgctgttcga aaagatcgcg ccacgcttcg ttgaccgtcc cggtggttat
     2941 acccgaatcg ttcgcctagc caagcggcgg gtcggggatg caggtgtgaa ggcgttcatt
     3001 gagttcgtgg gccaaaacga tcgcgtcaag caacgaaaga ctatccgtcc agaattcagc
     3061 gtcgaaacac ccaaaccgtc ggaacccgcc cctgtgaaag aggccacgat cgatacccca
     3121 gaagctgcga acgtttccca agagaatcca tcaaactgaa cgctgcgaac tctttctgcc
     3181 ctccccttcc caccccgccg gtttttcgaa accggcgggt tttttgttcg accgggccac
     3241 attaatcacg ggtttctcta gcacggaaga ttggaggcac atccattggg cgaggggtaa
     3301 acccattttt tattgcacgc catccccctc acccaccaga ccacccaact ggggagagtc
     3361 gcattgattt ttttgggaat tcttggtact gtcctgtccg cgctccatgg actagcgctg
     3421 acaggattct ttccatttgg cacagtactg tggacgagcc cccttctggg gagactcgaa
     3481 aagtcttcaa atgcggttac cgactggtga ttaccaaagg attgattgga agacctcgca
     3541 cgggatgaca acgagagttg ccgcccattt tgaacggatg tgtgccctcc tggtgattct
     3601 tgggatggcc agtggttgca cgcatctccg catgcccgcg agtagtcctt tggtcccagc
     3661 ccagatgtcc agcgacagcg tcgctctcga catcatcttt gcccacgtcc ctccctccga
     3721 tgacgtgcta aaccaaatct ggaccgagct cgacgaacaa ttcctgcccc aggaaact
//
LOCUS       DAOPTR010000285         3778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19434_length_3778_cov_3.502015, whole genome shotgun sequence.
ACCESSION   DAOPTR010000285 DAOPTR010000000
VERSION     DAOPTR010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19434_length_3778_cov_3.502015"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PKI05_06610"
     CDS             complement(<1..221)
                     /locus_tag="PKI05_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOA51910.1"
                     /translation="MKVLMLVGDYVEDYEVMVPFQALQMVGLTVHAVCPGKKAGEQVR
                     TAVHDFDGAQTYCELRGHNFTLNADFDQVR"
     gene            666..1679
                     /locus_tag="PKI05_06615"
     CDS             666..1679
                     /locus_tag="PKI05_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOA51911.1"
                     /translation="MARPVTMFTGQWADLPLETLLQKVKEFGYDGVELACWGDHFEVQ
                     KALQDDEYCAKKRELLNKYGMQVFAISNHLVGQAVLDNIDSRHKAILPDYVWGDGDPA
                     GVNQRAADELKDTARAAQKLGVSIVNGFTGSSIWHLLYSFPPVPPDMIEEGYRLLAER
                     FIPILDVFAECGVKFALEVHPTEIAFDLYTAERTLEALKGHEAFGFNFDPSHLIWQGV
                     DPAQFIRYFPDRIFHVHMKDAIVKLDGKAGILASHLNFGDWRRGWDFRSLGHGSVNFE
                     EIIRALNDIGYEGPLSVEWEDSGMDREFGAKEACQFVRKINFAPSRVAFDAAFDKAEQ
                     KKA"
     gene            1965..2597
                     /locus_tag="PKI05_06620"
     CDS             1965..2597
                     /locus_tag="PKI05_06620"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOA51912.1"
                     /translation="MLIYCIRHGESTYNSEGRVQGQSDVPLSDLGRRQGEATAEALAK
                     VGLEIIYSSPLRRAYETAEMVARRTGCDIRIDPRLMEIHAGIFQDRLRHELIREFPED
                     FRRWTSEDPDFVIPGGESRRQLAERGVAAFRDIAQSKFERVAVVSHGRLLVVTLKALL
                     VDSSHLIPPALQNASITVVEADPKTGQFRLVDVDQTDHLIGVGLSGRGDL"
     gene            2689..>3778
                     /locus_tag="PKI05_06625"
     CDS             2689..>3778
                     /locus_tag="PKI05_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOA51913.1"
                     /translation="MLLMRCAPLSVCILAIAGALVMGTSAFAETNVPAKPNIIFILAD
                     DLGYGDLGCYGQKLIETPEIDRMAAEGMRFTQFYAGATVCAPSRCVLMVGQHTGHCSV
                     RGNAGANNRSIQSLREDDITISEILKKQGYATALIGKWGLGEVEEPGHPLRKGFDYFF
                     GYLNQGHAHNYYPEFLWRNWGKVMLRNEVVATKADYGSDFKPGYATKRVDYSHDLFAE
                     DALRWVEENRNSPFFLYLAFTIPHANNEGTRGTGDGAEVPDYGIYADRDWPNQDKGQA
                     AMITRMDRDVGRLLNKLRELGLAEKTLVLFSSDNGPHNEAGHNPKRFQPSGPLRGMKR
                     DLYEGGIRVPLIAWWPGTIQAGTVTDHIA"
BASE COUNT          858 a         1030 c         1055 g          835 t
ORIGIN      
        1 cgaacttgat cgaaatcggc attcagcgtg aaattgtgac cgcgcagctc gcagtacgtt
       61 tgggctccat caaaatcgtg gacggcggtc cgaacctgct caccggcctt ctttcccgga
      121 caaacggcat gcaccgttag cccgaccatt tgcagggcct ggaacgggac catgacttca
      181 taatcctcaa cgtaatcgcc caccaacatg agcactttca tgacggaaaa cctcctccaa
      241 aaacctttct ttaaaaggtc cagcaatctt cccgagcaac caaagctgta gcaacgtatt
      301 ctatcgaatc gcctttcccg aaaaagggtt ccatcgctca atacggcttt ttcttggtag
      361 caagccgggc aaggaacgag tcgtgtcacc ggggcaaaac ccgcccggtc tggagcgacg
      421 aggccatctt taggttgctg aaaaacccta agggtggtgc aacatcgggt agcggggcaa
      481 tctttaccga ggccatgaga acgcaatgga cgaaaaaacg ggctcgcgat catgtggggg
      541 tgttgaactc cccacaaaat atgcacaaaa tacgaaccaa ggtttgtgtc agtcgcttgc
      601 tggcggccga acggtttcca attgtccagc atccgtggtc cttttgaaat gataaggagt
      661 caatcatggc gcgaccggtg acaatgttta cgggtcagtg ggccgacttg cccctcgaaa
      721 ctctcctcca aaaggtgaaa gagttcggtt acgacggggt cgagctggca tgctggggtg
      781 atcactttga ggtgcagaag gcgctgcaag acgacgagta ctgcgcgaaa aaacgagagc
      841 tgcttaataa gtacggcatg caggtcttcg ccattagtaa ccacctcgtg ggacaggccg
      901 tgctggacaa catcgattct cgtcataagg ccattctgcc cgattacgtc tggggtgacg
      961 gcgacccggc aggtgtgaat caacgggctg cggacgaact gaaagacacg gctcgcgccg
     1021 cccaaaagct cggcgtcagc attgttaatg ggtttacggg atcgagtatt tggcatctcc
     1081 tgtattcatt cccgcccgtc ccgccggaca tgatcgagga aggctaccgc ctgttggccg
     1141 aacggttcat ccccatcttg gatgtgtttg ccgaatgcgg tgtgaaattc gctctagaag
     1201 tgcatcccac ggaaatcgcc ttcgacctgt acaccgctga aaggacgcta gaggctctga
     1261 aaggtcacga ggctttcggg ttcaacttcg atccgagcca cttgatctgg cagggtgttg
     1321 accccgctca atttatccgc tacttcccgg atcgtatctt ccacgtccat atgaaagacg
     1381 ccatcgtcaa actcgatggg aaggctggca tcctggcaag ccatctcaat tttggtgatt
     1441 ggcgacgggg ctgggacttc cggtcgctcg gccacggtag cgtcaacttc gaggagatta
     1501 tccgagcatt gaacgacatc ggttacgaag ggccactctc tgtggaatgg gaggacagcg
     1561 gcatggaccg cgaatttggc gccaaagaag cctgccagtt cgtgcggaag atcaatttcg
     1621 ctccgagccg ggtagcattc gatgctgcct ttgacaaggc agaacaaaaa aaggcgtgat
     1681 cggataagcg cttcgactgt gtcgtgtgga aaaaaaggat tattgagggt tcttaaagtc
     1741 tatcccacaa cctcccctct cccgttcagc gggagagggg tcgggggtgg gggtggttcg
     1801 aatcgctgcc ggttttgtcg cttgactccg acgatcgttt gccctcaccc caaccctctc
     1861 ccagagggag agggaatttt gggataggtt cttaattgac acggtcggtt gccggccgtg
     1921 ccaattttat gcgcagacgc ttcttgccgt aatccgttca aaccatgctg atctactgta
     1981 ttcgtcacgg agaaagtacg tacaactccg agggacgtgt tcaaggccaa tcggatgtcc
     2041 ccctctctga cttaggacgc cgccaaggcg aggcgacagc agaggccctg gcaaaagtcg
     2101 ggctggagat catctattcc agtcccctgc gacgggctta cgaaacggca gagatggtgg
     2161 cccggcgtac gggctgcgac atccggattg accctcgtct gatggagatt catgcgggaa
     2221 tcttccaaga tcgcctacgg catgaactga tccgggagtt ccccgaggac ttccgacgct
     2281 ggacctccga agaccccgat ttcgtcatcc ccggtggcga atcgcgacga cagcttgcgg
     2341 aacgcggcgt tgcagcgttt cgggacatcg cccagtcgaa atttgagcgt gtggctgtcg
     2401 tttcgcacgg acgcctcctt gttgtgacgt taaaggcact tttggtagat agctctcact
     2461 taatcccccc ggcgctgcaa aatgcgtcga ttaccgtcgt cgaggcagat cccaaaacgg
     2521 gtcaattccg cctggtggat gtggatcaga ccgaccacct cattggcgtc gggctttcag
     2581 gtcgcggcga cttatgacaa gggttgcgat accttctcca aaagaacaaa atatttgctg
     2641 gtaaccttgt ttttggtctc cacaaccatc catcggggag gaacaggtat gttgctgatg
     2701 cgctgtgctc ctttatccgt gtgcattctc gccatcgcgg gtgcccttgt aatgggaacc
     2761 tccgcattcg ccgaaaccaa cgtgccggcc aaacctaata tcattttcat cttggcagac
     2821 gacctgggtt acggcgatct tggctgttac gggcagaagt tgattgaaac accagaaatt
     2881 gatcgcatgg ctgctgaagg gatgcgattt acccagttct acgccggggc caccgtatgt
     2941 gcgccgtcgc ggtgtgttct catggtcgga cagcacaccg gtcactgttc cgtccgcggc
     3001 aatgccggag cgaacaaccg ctcgattcaa tcgctgcgcg aggatgacat cacgattagt
     3061 gaaatcctga aaaagcaagg atacgcaacg gccctcattg gaaaatgggg attgggcgaa
     3121 gtcgaagaac ctgggcatcc tctccgcaag ggtttcgatt acttctttgg atatctcaac
     3181 cagggacacg cgcataatta ttatcccgag tttttgtggc ggaactgggg taaggtcatg
     3241 cttcgcaatg aggtagtggc caccaaggcc gattacggct cggacttcaa acccggttac
     3301 gccaccaagc gggtcgatta ttctcatgac ctgtttgcag aagacgccct tcgctgggtg
     3361 gaagaaaacc ggaactctcc cttcttcctc tatctcgcct ttacaattcc ccatgccaac
     3421 aacgagggga cacggggaac cggcgatggg gccgaagtgc ccgattacgg catctatgcc
     3481 gatcgagatt ggcccaatca agacaaaggc caggcggcca tgatcacccg catggaccgc
     3541 gacgttgggc gactcctcaa taagttacgg gagttgggac tggccgagaa aacactggtc
     3601 ttgttttctt cggacaacgg tccccacaat gaggcgggcc acaatcccaa gcgattccaa
     3661 ccgtcggggc cacttcgcgg gatgaagcgc gacctttacg agggtggtat ccgcgtcccc
     3721 ttgatcgcct ggtggcccgg gacaattcag gcgggcacag tcactgacca tatcgctt
//
LOCUS       DAOPTR010000286         3776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19444_length_3776_cov_3.317657, whole genome shotgun sequence.
ACCESSION   DAOPTR010000286 DAOPTR010000000
VERSION     DAOPTR010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19444_length_3776_cov_3.317657"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PKI05_06630"
     CDS             complement(<1..462)
                     /locus_tag="PKI05_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HOA51914.1"
                     /translation="MAQSELNVTGSRTATDGTLPEGAIPSLARDVDPVETQEWLDALE
                     AVIRREGPERAVYLLERLKEKAFRTGVPWVSSATTPYINTIPPEAEPEYPGDRALERR
                     IKSLIRWNALAMVVRANRVSSGIGGHISTFASAATLYEVGFNHFFRGKQGPN"
     gene            complement(755..1657)
                     /locus_tag="PKI05_06635"
     CDS             complement(755..1657)
                     /locus_tag="PKI05_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOA51915.1"
                     /translation="MPLVVVGSVALDSVETPHERRENVVGGSAVYFGLAARFFTPVRL
                     VGVVGEDWPPEHTQLLQDHGIDTSGLMTIPGAKTFRWRGRYLPNMNDRETLEVQLNVM
                     SQFDPVLPEAFRRCKFLFLANGSTKMQMRVLDQCVGPDLVVADTMDHWIRNEPEELRE
                     LMQRIDGLVLNDAEAKLLAQDENLVRAGKKILEFGPTFVVIKKGEHGAMFFSRHEMYV
                     LPAYPTSAVVDPTGAGDSFAGGMMGYLAEKNDFSPRTLKEAMAYGVVVASFTVEDFSI
                     DRLLKIRREDIQLRLDEYRKMLMF"
     gene            complement(2459..>3776)
                     /locus_tag="PKI05_06640"
     CDS             complement(2459..>3776)
                     /locus_tag="PKI05_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOA51916.1"
                     /translation="PRARSKAYSLLRSILNAAIELLKKVRDGRLRLDRTMDVSVTNLR
                     GKRHIIHLLGPNLKTLEEIQRRNRRDFRIMMSRRESLENRLAARRRLMKRRFRAVRLV
                     EELDLRTQKLQPLLDRLTRISQRMDEIVRNLKSLEGTPETPWAKELRRELRYLMRMTL
                     ESPTTLRKHLAAINSYAQEYERAKRYLSAGNLRLVVSIAKRYRNRGLSFLDLIQEGNT
                     GLMRAVDKFEYARGFKFSTYATWWIRQAITRAIADQSRTIRMPVHMIDLCGRIHATAR
                     KLAQETGVIPTLDETAQALRMSVEDVRSILRMSHQPLSLDQPVGEQEESYLGEFLADY
                     REDDPLYEMNFELLKSSIQDALSDLTYREREIIRLRYGLADGYAYTLEEVGKIFGITR
                     ERVRQIEAKAIRKLRHPVRSRKLAGFVDALVARGQTTPDPALDREL"
BASE COUNT          832 a         1081 c         1032 g          831 t
ORIGIN      
        1 attaggacct tgcttgcccc ggaagaagtg attgaacccc acctcgtaaa gcgtggctgc
       61 cgaggcaaaa gtcgaaatat gcccgccaat tcccgagcta acccgattgg cgcggacgac
      121 catggccaag gcgttccagc gaattaggct cttgatacgg cgctccaggg cccgatcgcc
      181 tggatattcg ggttcagcct ctgggggaat cgtattgatg tacggtgttg ttgccgagga
      241 gacccacggg acgccggtgc ggaaagcctt ctctttcagt cgttccagca gatacacagc
      301 ccgctccggg ccttcacgcc gaatgacagc ctccaaggcg tctagccatt cctgggtttc
      361 cactgggtca acatcacggg ccaacgaagg aatagcccct tctggtaagg tcccatcggt
      421 agcagtacgc gatccggtca cgttcaactc gctttgggcc atactcactt gcctcgtttt
      481 catttcacaa gaatacccag tccatcttgc ccaatcagcc tacactacgg caatgatcct
      541 gacgaccgcc gatcaaccca atcccgagca ccggatggaa gattcatcgg tcggcttcat
      601 ggcgatgttt gaaacaccat gaattatacg aactcggcca aaaacacttt ttttccctac
      661 cggaacggat gcatcgtttc tactcggcga atgcttccac agtgggcgga acgagattcg
      721 ccacggtgtg ggtgttttcg gcaaaagcgc ttttctaaaa catgagcatc tttcgatatt
      781 catccaatcg cagttggata tcttcccgcc gaatcttcaa aaggcggtcg atactgaagt
      841 cctccacggt gaagcttgcg acgactacgc cgtacgccat agcttctttg agtgtccgtg
      901 gggaaaagtc gttcttttcg gcgaggtagc ccatcatacc gccagcaaaa ctgtcccctg
      961 cacccgttgg atccactact gccgacgttg gatatgctgg gaggacgtac atctcgtgcc
     1021 gactgaaaaa catggctccg tgttcgcctt tcttgataac aacgaacgtc gggccgaact
     1081 cgagaatctt ctttcccgcg cggacaagat tctcatcctg agctaacaat ttggcctcag
     1141 catcgtttag gaccaggcca tcgatccgtt gcatcaattc ccgaagttct tctggttcgt
     1201 tacgtatcca gtggtccatg gtatcggcca cgaccagatc gggcccaacg cattgatcca
     1261 ggacccgcat ctgcatcttc gttgagccgt tggccaggaa aagaaacttg caacgtcgga
     1321 aggcctcggg gaggactgga tcgaattgcg acatgacgtt gagctggacc tcgagggtct
     1381 cgcggtcgtt catgttcggc aggtaacggc cccgccagcg gaatgtcttg gcgccgggaa
     1441 tcgtcatcag tcccgacgtg tcaatgccgt gatcctgaag cagttgcgta tgttccgggg
     1501 gccagtcttc ccctacgaca ccgacgagcc gaaccggcgt aaaaaatcgg gcagccaaac
     1561 caaaataaac agccgatcct cccaccacgt tttcgcgtcg ttcgtgggga gtctccacac
     1621 tgtctaaagc gaccgagcca accacgacaa gcggcatgga acgaggtctc ctaactcaca
     1681 ggtctcatct tgatttggac aatcaccggg ttcaaaaccc ttttgggtat gggacgcctc
     1741 gcgccccgca ctgtctttgt aagaatactg gtttttgccg tggttcgcta gcgtcggcca
     1801 cgtggcggat taggtgctgc gaattggaag aggagcggtc gggaatttct ccgataattg
     1861 gagatgtcac cgatagcgtc aaaatgatag gacggcctat cgtgagccac cggcgcgatg
     1921 accaagcaat tgctcgcaac ttcgatcaat taagagccta tcccaaaaag tccccctctc
     1981 cctccgggag agggttgggg tgagggcaag cgaacgtcgg gatcgatgga gaaaacgggc
     2041 agcgattcga ggcaccttca cccccgacac ctctcccgcc gaacggataa gaagagatta
     2101 tgggataggc tctaagtacg acattgatct tgggcaagcc aggaagggcg agagacctcg
     2161 caccttccac cccgaaaacc ctattgcagg tcactgcaag ttctgccagc gagatagaac
     2221 aggaatattt cagggcttaa cgacgaccac aacccattga gctcccacaa attgcggaca
     2281 tcaagaaagc tgcggcgtgc gaggactttt ttcctacggc atggcccagc gatcgtacga
     2341 acacggaggc cgcgaggatt ccgcagaagg ggcgaggcct cattcagcgg ccgccttgcc
     2401 aagtccacaa ctcatgcgag attctgtggg agggaccaag tcccctcggt ctcgccactt
     2461 acaactcccg gtcgagggcc ggatcgggcg tggtttgtcc gcgggcgacg agggcatcga
     2521 cgaagcccgc cagttttcga gatcgcaccg gatgccgcag ttttcggatg gccttggcct
     2581 cgatctgccg gacccgctcc cgcgtgatac cgaagatctt gcccacttct tccagcgtgt
     2641 aggcataacc atcggccagc ccataacgca accggatgat ttcccgctcc cgatacgtca
     2701 ggtccgaaag ggcatcctga atggacgact taagcagttc aaagttcatc tcgtagaggg
     2761 ggtcgtcttc gcggtaatct gccaggaact cgcccaaata gctctcttcc tgttcaccaa
     2821 ccggttgatc gagtgacaat ggctgatggc tcatacgaag gatactgcga acatcttcga
     2881 ccgacatcct cagcgcttgg gcggtctcgt cgagggtcgg aatcacaccc gtctcctggg
     2941 ccagcttgcg agccgtggcg tggatgcgcc cacagagatc gatcatatgc acgggcatcc
     3001 ggatggtgcg actttggtcg gcaattgccc gggtgatcgc ctgtcgaatc caccatgtgg
     3061 cataggttga gaatttgaag ccgcgggcat actcgaattt atccaccgcc cgcatcaatc
     3121 ccgtgttacc ttcctggata aggtcgagaa agctcaatcc tcggttgcga taccgtttgg
     3181 cgatggagac gacgagccgg aggtttcctg ccgaaagata ccgcttcgcc ctttcgtatt
     3241 cctgggcata ggagttaata gcggcaagat gtttccgcaa tgtggtgggg ctttccaagg
     3301 tcatccgcat cagatagcgg agttcccgcc gcagttcctt ggcccagggg gtttctggcg
     3361 tgccttccaa ggatttcaga ttgcggacaa tttcatccat tctttgcgaa atccttgtca
     3421 gccgatccag caatggctga agcttttgcg tccgcagatc gagttcttcc accagtcgga
     3481 ctgcccgaaa ccgacgcttc atcagccgtc gccgagccgc taaacggttt tccagcgact
     3541 cgcggcgact catcatgatg cggaaatcac gtcggtttcg ccgttgaatc tcctcgagag
     3601 tctttaagtt tgggccgaga agatggatga tatggcgttt accccgcaga ttggtcaccg
     3661 agacatccat ggttcggtcg agccgcagac gaccgtctcg gacctttttc agcagttcga
     3721 tcgcggcgtt gaggatggat ctgagcaagg agtaggcctt ggagcgggcg cggggg
//
LOCUS       DAOPTR010000287         3772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19470_length_3772_cov_3.452515, whole genome shotgun sequence.
ACCESSION   DAOPTR010000287 DAOPTR010000000
VERSION     DAOPTR010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19470_length_3772_cov_3.452515"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            744..1448
                     /locus_tag="PKI05_06645"
     CDS             744..1448
                     /locus_tag="PKI05_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51917.1"
                     /translation="MAIFVVCPGCRTRFTVSDQFAGRSGPCPKCKTIIQIPKLNEQVV
                     IEEPEQFADGGRGISGQLTTKPLARVETAIKPVHILIAVGAFVIVLVVTLVLGQLDIF
                     HQQVWLHAIGLVLVGVPAAAMSYEFLRHQDDLKPLRGRNLIIRSLACAVGYLIIWGLF
                     NWLVANFVTEELWTWAIVLPPAFAAGGFVAYAAMELDYSAGILHFTFFSLIALVLRWA
                     AGFGWIWNLPQPVYPT"
     gene            complement(1495..3588)
                     /locus_tag="PKI05_06650"
     CDS             complement(1495..3588)
                     /locus_tag="PKI05_06650"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOA51918.1"
                     /translation="MRASIQEVILGPLNPQQREAVQHGEQPLLIVAGAGTGKTATIVH
                     RVAWLVAHGVSPQSIMLLTFTRRAAEEMIRRASSLLSRLGLSRESTHTSHIWGGTFHA
                     IGLRFLRQLGHHIGLPANFTVLDRSDAEDLMNVVCSELKFESMDRRFPKKGTCLDIYS
                     RMVNTGEPLETVLESTFPWCRSWHSELAELFEAYCERKEEYATLDYDDLLMQWLKLLS
                     HSQIAEHIRKRISFVLVDEYQDTNPIQAQILYALSPSGQGVTVVGDDAQSIYSFRGAT
                     VKNILDFPKHYPSARIVTLEQNYRSTMPILRAANAVMASAKERFTKNLWSEQRAGCLP
                     RLVTCDDEYEQADLIIREVLAHHELGTDLRRQAVLFRASHHSLPLEAELTRREIPYRK
                     YGGLKFLEAAHIKDLLAFLRLAENPRDVVAGVRVLCLLPGIGPAKAQKLLEQVASSPQ
                     PSFDPWKEINPSRDSADIWPSFVSVMEDVASRNLPVAAQIHRVRIFYEPMLPVLYDNP
                     IPRAKDLEQLEQIASRYKDRQTFLAQIAIDPPNSTQDFAGKPDLNDDFLILSTIHSAK
                     GLEWDVVYIINATDGHIPSDMATGSEAEIEEERRLFYVAMTRARRVLYVYTPVRYYVS
                     PSFADSRYGMGLPSRFLTPEVVECFERYHGDVLDGEMQANYQQYPNDLPSGIGKPIFA
                     RRSQKAGGDISMESG"
BASE COUNT          847 a          956 c         1061 g          908 t
ORIGIN      
        1 cctatcggcg gtgatcccgg tttcttggtc gggcgtccaa agccctcggt tctgggcctc
       61 cgagggagca gcaaaccata tgatgaccaa cagaccgatg gccagttggg agaaatgttg
      121 catgcgtcca accgagagcg ccgagcgagt taccttcatt gttttttacc tcatgcggat
      181 tgttattgtt gacgtgaaat atctgatctc cgtattcgcc aaacaccctc gcaggtttgg
      241 agtgctcatc aaactgtgcc tatcgtacga ttcgagcaca gcctatgcaa gcgttctagc
      301 ttggtagcga ggcgtttgtg atgtaaccct cacgcatggg aagcagtttt acgcccggat
      361 tcgcatccga tttggacaaa ttccatagct gccaaacgat ttggccattc agttttgctc
      421 gcggattacc atccgatttg aagaaatacg acgcactttg taccaatttt ggagggaggt
      481 gtgccgttac ttcgcgtgtg gagcctattg gtgatccatc atgttcgccc accacgagcg
      541 cgcgtgagtt tcaaaggtaa gcacatcaga tggttacaac gaagtgggcc ggttgtgctg
      601 ggccaccacg aagaaagtga tcttgatttt gttcgggctc gtcgacgctc aagcgcgcct
      661 agccggcggt tgacaaggga aacacctctg ccaaactaaa agagacagca ggatggtcat
      721 ttcttgagga catgagggtt cttatggcta ttttcgttgt ctgtccggga tgtcgcactc
      781 gtttcactgt cagtgatcaa tttgctgggc gcagcggccc ttgtcccaaa tgtaagacta
      841 ttatccaaat cccgaaactg aacgagcagg tcgtcattga ggaaccggag cagtttgccg
      901 atggaggccg gggtatctct gggcaactaa caacaaagcc gctcgcccga gttgagacgg
      961 cgattaaacc cgttcacatc ttgattgccg tcggggcgtt tgtgattgtc ttggtcgtta
     1021 cgcttgtctt gggccagttg gatatcttcc atcagcaagt gtggcttcat gctatcggac
     1081 tagtcctcgt gggagttcct gcggcggcga tgagttacga gttcctccgt catcaagatg
     1141 atctaaagcc gcttcggggg agaaacttga tcatccgttc tctggcgtgt gcggtgggtt
     1201 atctcatcat ttggggattg tttaactggc ttgttgccaa ttttgtgacc gaagagctgt
     1261 ggacgtgggc gattgtctta ccgcccgcct ttgctgctgg aggattcgtt gcttacgcgg
     1321 ccatggaact ggactattcc gccgggattt tgcattttac gtttttctcg ctgatcgcac
     1381 tcgttctacg ttgggcggca ggctttgggt ggatttggaa cttgccgcaa cccgtctatc
     1441 ccacgtagtc acccggcgag ttggctgatg gaaggcactc ttgccctgac catctcagcc
     1501 cgattccatc gagatgtcgc ctccggcctt ctgcgatctg cgagcaaaaa tgggtttgcc
     1561 aatgcccgat ggcagatcat tcggatactg ttggtaattt gcttgcatct cgccgtcaag
     1621 cacatcgccg tggtatcgct cgaaacactc gacgacctca ggtgtcagga atcggcttgg
     1681 aaggcccatg ccatagcggc tgtcggcaaa agatggggat acgtagtacc gaaccggggt
     1741 atagacgtaa agaacgcgtc tggctcgcgt catagcaaca tagaataggc gtcgctcttc
     1801 ttcgatttcc gcctccgagc cggtagccat gtcggaaggg atgtggccgt ctgtcgcatt
     1861 gatgatgtaa acgacgtccc attcgaggcc tttggctgaa tgaatcgtac ttaggataag
     1921 gaagtcgtca ttcaaatcgg gcttgccggc gaagtcttgc gtggaattcg gcggatcaat
     1981 ggcaatctgg gcaaggaatg tctggcggtc cttatatcgg gaggcgatct gctccaactg
     2041 ctccaaatcc ttggcgcgag ggatcggatt gtcgtacaac acgggtaaca ttggctcgta
     2101 aaagatccgc acccgatgga tttgggccgc aacggggaga ttgcgactcg caacgtcctc
     2161 catgacggag acaaacgatg gccagatatc ggcagaatca cgggatggat tgatctcttt
     2221 ccaaggatcg aaggaaggtt gcggagaaga tgccacctgc tccagcaact tctgagcctt
     2281 agccgggcct atgccgggaa gcaaacaaag gaccctcacg ccggcgacca catcgcgggg
     2341 gttttcggca agccggagaa atgccagaag gtccttgatg tgggcagcct ccaggaactt
     2401 aagtccccca tatttgcgat agggaatctc ccggcgtgtt aactcggctt ctagggggag
     2461 gctgtggtgc gaggcccgaa agagcacggc ctggcgtcgc aaatcagtgc ccagctcatg
     2521 atgagcgagg acttcgcgga tgatcaaatc ggcttgctca tattcgtcat cacaggtaac
     2581 caggcgcggt aaacaacccg cccgctgttc tgaccatagg ttctttgtga agcgttcctt
     2641 cgccgacgcc atgacggcat tggccgccct caggatgggc atcgtgctgc ggtaattctg
     2701 ttcaagggtg acaattctcg ctgagggata atgcttggga aagtcgagaa tgttcttgac
     2761 tgtggcacca cgaaaagaat aaatcgactg agcgtcatcg ccaacaacag tgacgccttg
     2821 tccagaagga gataaggcgt aaagaatctg ggcctgaatt ggattagtat cttggtattc
     2881 atcgacgagg acaaacgaaa tccgtttgcg gatatgttct gcgatttggc tgtgggacaa
     2941 tagctttaac cattgcatga gcaaatcgtc gtagtccaag gttgcgtatt cttcctttcg
     3001 ttcacaatac gcctcgaaaa gctcggccag ttcggagtgc caactgcggc accaaggaaa
     3061 agtactctcc aacaccgttt ccaatggttc gcccgtattc accatgcggc tgtaaatgtc
     3121 gagacaggtc cctttcttcg ggaatcgcct gtccatgctt tcgaatttca actcggaaca
     3181 aaccacgttc atcaaatctt ctgcgtcgga ccgatcaagc acggtgaagt tggcgggcag
     3241 accgatatgg tgccccagtt gtctgaggaa tcggaggcca atggcatgga atgtccctcc
     3301 ccagatgtgg gacgtatggg ttgattcccg cgaaagaccc aaacggctga ggagtgaact
     3361 ggctcgtcgg atcatttcct cagcggcgcg gcgcgtgaat gtcaggagca taatcgactg
     3421 tggggagacc ccgtgggcaa cgagccaggc tacccgatgg acaatggtgg cagtcttccc
     3481 cgtacccgct ccagcgacga tcaaaagggg ctgctcacca tgctggacgg cctcccgctg
     3541 ttggggattc aaaggcccca agatgacctc ctgaattgac gctcgcatag ccttcctctt
     3601 tgagtgtgac gaggcgattc gccatttgct aaccaagcgg ggagaccttc acctgccaac
     3661 gcccggatgg agaatataga atcattataa agcttgcgca gtgtggtcaa aagcccaaaa
     3721 ggagcggcaa agtgccattg tttcttacaa ttgaaacaac atgcgatgaa ac
//
LOCUS       DAOPTR010000288         3761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19549_length_3761_cov_3.407717, whole genome shotgun sequence.
ACCESSION   DAOPTR010000288 DAOPTR010000000
VERSION     DAOPTR010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19549_length_3761_cov_3.407717"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..918
                     /locus_tag="PKI05_06655"
     CDS             52..918
                     /locus_tag="PKI05_06655"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOA51919.1"
                     /translation="MRRREFLAAAGAGVALSTFPLRWTHGAFSAPKKILYFTRSAGFE
                     HSVVARKGGELAFSEKILIQWGKEVNVEVVCSKDGGVFDGDLDQFDAFAFYATGDWTK
                     PVDRPNEPPATESGVKRLLEAIAAGKGVIGFHAANDAFHSRGNEISPFIKMLGGEFAS
                     HGRQQEATMRVVSPKFPGCEKLGDSFRMMEEWYAMKNLADDLHVILVQETEGMQDACY
                     QRPPYPATWARMHGKGRVFYTSLGHREDVWESSIMKQISLGGLAWVLGEVNFDPVPNI
                     KEVTPGANQLPK"
     gene            1039..2010
                     /locus_tag="PKI05_06660"
     CDS             1039..2010
                     /locus_tag="PKI05_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOA51920.1"
                     /translation="MSFAALSRRRFGQIVAGAASVAVFTCEPLQAKRIPEVTSPLEAF
                     RFCLNTATIRAQRLPLAEEIDLAAKVGYHGIEPWTREIEEYISSGGKLADLRKRLQDR
                     GLSVESAIGFPTWMVDDDTQRANGFEQMKREMDWIAQLGGTRIAAPPAGAYNSPPIDL
                     RRVAERYAQLLDLGHSMGVVPQLEIWGASANLSRLSEAAFVLVECGHPDASALFDVFH
                     IYRSGSPVAGLRVFNGRNLHVFHMNDYPADPPREKIRDSDRVFPGDGVAPLSTILQIL
                     AEIGFQGTLSLELFNPEYAKMTAEEVAQTGLAKMKNAVLTAFQKESS"
     gene            complement(2108..>3761)
                     /locus_tag="PKI05_06665"
     CDS             complement(2108..>3761)
                     /locus_tag="PKI05_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HOA51921.1"
                     /translation="TGLACTVFQRMNGQGDMLRVCTNGPGTDGGRAIGTYIPATNPDG
                     TPNPVVSTLLKGQAYVGKAVVVNDWYITKYEPIFNDKKEVIGCLYVGIRRDDVPEVRQ
                     GIMDITVGKTGYVYVLQGTGEKRGTYIISQGGKRDGECIWEAKDADGHYFIQSIVEKA
                     LKLKPGECAFERYPWKNADDKTARWKVAAVTYFEPWDWVIGAGAYEDDFQDALGRVND
                     ALDSMLWWTLVGAAVVVLGCCGFTYYATRRSLGGIHKAVDMLKDIAEGEGDLTKRLPA
                     TGNDEIAQLAKWFNVFIGKLQEMIHQVVVSADQFTEGARVVAESSQTVAQGAQTQAAS
                     VEQMTASIEELVRSVDSIRQLASEADQLARRSSQSAEHGGVAVEKSISAMNAIRESSK
                     RIGDIIQVISEIAGQTNLLALNAAIEAARAGEHGMGFAVVADEVRKLAERSNRAAQEI
                     AQLIRESTRLVEEGAQMSDTLGESLREILEHIKETAAKVTAIAEKTTEQAAAAQEVRN
                     AVQGIAQVAEQSAAGSEEMASSSEELGAQADALRQLVAGFKT"
BASE COUNT          771 a         1089 c         1026 g          875 t
ORIGIN      
        1 tccgatacgc gtcttccctg tcatgcgttt ttcgagaaag gagttagcat catgagacgt
       61 cgcgagtttc tggctgcagc gggtgccggt gttgccctct cgaccttccc cttgaggtgg
      121 acccacgggg cgttcagtgc acccaagaaa atcctctatt tcacgcggtc ggcgggtttt
      181 gaacattccg tagtcgcccg caaaggtggc gagttggcct tctccgaaaa gatcctcatc
      241 caatggggca aggaagttaa cgtcgaggtt gtctgcagca aggatggcgg ggtcttcgat
      301 ggcgatttgg atcagtttga cgcctttgca ttttacgcca caggtgattg gaccaagccg
      361 gttgatcggc ctaacgagcc gccagcgacg gaatccgggg tcaagcggct tctagaagcg
      421 attgccgcag gcaagggcgt catcggattc cacgcagcca atgatgcctt ccatagccgc
      481 ggcaacgaga tttccccgtt catcaagatg cttggcggcg aattcgcctc tcacggtcgc
      541 cagcaggaag ctaccatgag ggttgtttct cccaagttcc ccggctgcga gaaactcggc
      601 gactccttcc gcatgatgga agaatggtat gccatgaaaa acctcgcgga tgatctgcac
      661 gttatcctgg ttcaagagac tgaaggcatg caggacgcct gctatcagcg cccaccttat
      721 cctgccactt gggcgaggat gcacggcaag ggacgggtct tctacacctc attaggccat
      781 cgcgaagatg tttgggaaag ctccatcatg aagcaaatca gcctgggtgg cctggcctgg
      841 gtattaggcg aagtgaattt cgaccccgtt cccaacatca aggaagtgac acctggagca
      901 aaccaacttc ccaagtgatc tcagttagtt agacgcattt gagtgtggta gtcggagcag
      961 ggggcgatgc agttgcgccc cctgttctgt ccgatgtggc ggtttttcgg ataggaattg
     1021 tgacttggag taaagattgt gagcttcgca gcgctctctc gacgacgatt tggccagatc
     1081 gtggctggcg ccgccagtgt tgcggtcttc acgtgtgagc ccctccaggc taagcgaatt
     1141 cctgaagtaa cgtcgcctct tgaggcattt cgcttttgcc taaatacggc cacgatccgg
     1201 gcgcagcgtt tgccattggc tgaagagatc gatttggccg ccaaggtggg ctatcacggc
     1261 attgaacctt ggactcggga aattgaggag tacatttcct cgggcggcaa actcgccgat
     1321 cttcgcaaga gactccaaga tcgcgggtta agtgtggaaa gcgccatcgg ttttcccacg
     1381 tggatggtcg atgacgacac ccaacgggcc aatggcttcg agcagatgaa gcgcgagatg
     1441 gattggatcg ctcaattggg gggaacacgg attgcggcgc caccggcagg ggcctataac
     1501 tcaccgccta ttgacctccg tcgtgtggct gaacgttatg cccaattgct ggatttgggt
     1561 cactccatgg gcgttgtccc tcaattggag atttggggtg catcagccaa cttgagtcgc
     1621 ttgagcgagg cggcttttgt attggtggaa tgcggtcacc cggatgcctc cgcccttttt
     1681 gatgtatttc acatctaccg aagcgggtcg cccgtggctg ggctacgggt gttcaacggt
     1741 cggaacctcc acgtgttcca catgaacgat tatcctgccg atccaccacg tgagaagatc
     1801 cgcgactccg atcgtgtctt cccgggcgac ggtgtggccc ccctgtccac gattctgcaa
     1861 atcctggccg agattggctt tcaaggcaca ctttctttgg agcttttcaa ccccgaatat
     1921 gccaaaatga cggccgagga ggtggcgcag accggtcttg cgaagatgaa aaatgccgtt
     1981 cttacggcgt ttcaaaagga aagtagctga actaatcgat tcgcaccgca tagaagggtc
     2041 accctggcgg gaaacaatgc ccagccaggg tgacgtatta cactcggcga gtttgaaagg
     2101 tctcgaatca ggtcttaaat ccagcgacga gttgacggag ggcgtcggcc tgggctccca
     2161 actcctcgct gctcgaggcc atctcttcgc tacctgctgc cgactgctcg gcaacctggg
     2221 caatgccctg gaccgcgttg cggacttctt gggccgccgc cgcctgttcc gtagtcttct
     2281 ccgcaattgc cgtgaccttg gccgctgtct ctttgatatg ctccagaatc tcgcgcagcg
     2341 attctccgag ggtatcgctc atttgggcac cctcttcgac aagtcgggtc gattcacgaa
     2401 tcaattgagc gatttcctgg gcggcacggt ttgaacgttc ggccagcttg cggacctcat
     2461 cggcaaccac ggcaaagccc attccgtgtt cgcccgcacg tgccgcctca atcgcggcat
     2521 taagagccaa taggttggtc tgtccggcaa tctcggaaat cacctgaatg atatcgccga
     2581 tgcgttttga actttcgcga atggcgttca tggcggagat tgacttttcg acagccacac
     2641 cgccgtgttc cgccgattgg ctacttcggc gggcgagctg atcggcctcg ctcgccagtt
     2701 gacgaatcga atccaccgat cttaccagtt cctcgatcga ggccgtcatc tgctcgacgc
     2761 tagccgcctg cgtctgagcc ccttgggcga ccgtctgcga gctttctgca acgacgcggg
     2821 caccttctgt aaactgatcg gcactcacga cgacctggtg gatcatctcc tgaagctttc
     2881 cgataaacac attaaaccac ttggcgagct gagcaatctc gtcattgcca gtggcgggaa
     2941 gacgttttgt gagatcacct tcaccctcgg caatgtcttt caacatatcc acggctttgt
     3001 ggatcccgcc gaggctccgc ctggtggcgt agtaagtgaa accacagcac cccagtacca
     3061 caacggccgc acccacaagc gtccaccaca gcatgctgtc cagggcatca ttaacccgcc
     3121 ccagagcatc ctggaaatca tcctcataag ccccggcacc gatcacccag tcccacggtt
     3181 caaagtatgt cactgcggca accttccaac gagccgtttt gtcgtcagcg tttttccagg
     3241 gatagcgttc aaacgcgcac tcacccggct tcaatttgag tgccttctcg acgattgact
     3301 ggatgaaata atggccgtcg gcgtccttgg cctcccagat gcattctccg tcgcgctttc
     3361 cgccttggga aatgatatat gtgcctctct tttcacccgt cccctgcaac acataaacgt
     3421 agcccgtctt cccgacagtg atatccatga tgccctgacg cacttcgggc acgtcgtccc
     3481 gacgaatccc cacatacaag caaccgataa cttctttctt gtcgttgaaa atcggttcgt
     3541 acttcgtgat gtaccagtca ttgaccacaa ccgctttacc tacgtacgcc tgccctttga
     3601 gaagcgtact gaccacaggg tttggcgtac cgtcagggtt tgttgccggg atataggtac
     3661 cgatggcacg gccaccatcc gtgcccggac cgtttgtgca gacgcggagc atatcgccct
     3721 ggccattcat tctttgaaag acagtgcacg caagtcccgt c
//
LOCUS       DAOPTR010000289         3757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19580_length_3757_cov_3.676121, whole genome shotgun sequence.
ACCESSION   DAOPTR010000289 DAOPTR010000000
VERSION     DAOPTR010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19580_length_3757_cov_3.676121"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..1066
                     /locus_tag="PKI05_06670"
     CDS             35..1066
                     /locus_tag="PKI05_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOA51922.1"
                     /translation="MASDFRLKERLPYITDRLVETYRTHGSTSHLGHCPLPNYEAVIQ
                     ATEWMKEILFPGYRRRDGLHMGNVTYYVGDLIDKLHDLLTQQIARALRHEANHSADCD
                     PVRLADFEALGQAKTLAFLEQLPELRKILALDVQAAYDGDPACKSLDEVILCYPGFEA
                     VTVYRLAHLLHELQVPLVPRMMTEWAHSRTGIDIHPGAKIGTYFFIDHGTGVVIGETC
                     EIGNHVKLYQGVTLGALSFPTDAEGRLVRGTKRHPTIEDRVVIYANATVLGGRTVVGH
                     DSVIGSSVWLTRSVPPHSTVTLERPNLRIFGERCEDLKPEASLADAPRTSAKATAGGN
                     GAAPGTTGH"
     gene            complement(1090..>3757)
                     /locus_tag="PKI05_06675"
     CDS             complement(1090..>3757)
                     /locus_tag="PKI05_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51923.1"
                     /translation="RQGHLPLWNPYLNFGAPLAAENSCAAFYPGIVILMLPIPFSAAY
                     LVFLIVHLCLAAWGIARLARRCGATPVAASLTTLSYPLSGYVLFQIYNPIFLISAAWL
                     PWAIHALFDIFFERNRLTRQSTDNDHATKGNTIHSWLHFLPPVQARSAIRLGVFLALM
                     ILGGDPHTAYHCAVLAFATWIVLIFSALGRRSNGERNRQPLARVLRRGLLLIGAALVT
                     LLLAAIQIIPTWTYTRLSERALLKPAASDSGNRLTERQDDKTAKSTEQNTLAEIARSR
                     QVSQWQRVIYDFSTPAYRWIEFVWPEFGGRPLPWNTRWIGAIVSEGRWWTPSLYMGLL
                     PFLLAILTMRFWPARRSFSSTGSDARYCESSQRNSPNMPPEAFRIWFSWVTVFSLVAA
                     LGWYGPAGVYGELWAWITGHSSNGTVSPPVGGLYWVVTQILPGYGLFRYPSKWLVLTT
                     LGLSVLASLAIDHLDTKYLRRLTRLLILILAWATCFLLVALFASHLWEYASQYVPQDP
                     VFGFFEPGRARWETVTGLVLLLALLLTWLCLVRWVPIRHLPIVIVLLTGLDLVLHNSW
                     MIFPVHVGKETQMALHSLPPFRSWQKWCDYPREWQTPSCTRIQELFDWERETSAIRWG
                     MLTGRAPLESYGTLVPGDYFVFLAILHEYMNRQKLKIPPQETLALLGALTTDAFLESN
                     QQEKTSRPDSPTARESHYCFLTHNVTKFPSELSARLNVWQKTEWVFFPNGRPRGPLDT
                     FVVESIEPCGLAILEQIPPKAAQTQERTTKEDCHVKAFQPGKVVIYASLQEPGIVVLR
                     EQFMPGWRVHVVSQDGSTSWDARPLRINRIMMGVALPPGKWQITWTYYDSGFFLGMAL
                     SAVAWLGIALMVVLPYLMRISRRLLCQRQGSR"
BASE COUNT          923 a         1021 c         1074 g          739 t
ORIGIN      
        1 gcaccaaacc cgtacttcaa accgaggatg agacatggct tctgactttc gacttaaaga
       61 aagactccct tatatcaccg atcgactggt agagacctat cggacacacg gatcaaccag
      121 ccacctggga cattgcccct tgcctaatta tgaggctgta atccaggcga ccgagtggat
      181 gaaagaaatc ctgttcccag gctatcggcg tcgtgacggt cttcacatgg gcaacgtgac
      241 ctactatgtc ggcgacctga tcgacaaact tcacgacctc ttaacccaac agattgcccg
      301 cgctcttcgg cacgaagcca accattccgc cgattgtgat cctgtccggc tggccgattt
      361 cgaagccttg ggccaggcca agacgttggc cttccttgag caacttccgg aactgcggaa
      421 gattctggcg cttgatgtgc aggccgccta tgatggcgac cccgcgtgca aatcgttgga
      481 cgaagtcatc ctttgctatc cgggatttga ggccgtcaca gtgtaccggc ttgcccatct
      541 cctccacgag cttcaggttc cgttggtgcc ccgcatgatg acagagtggg cacacagccg
      601 gaccgggatt gatatccacc cgggagcgaa gatcggtacc tatttcttca tcgatcacgg
      661 aactggggtt gtcatcggcg agacgtgcga gattggaaac cacgtcaagc tttaccaagg
      721 cgtgacactt ggggccttga gttttcccac ggatgcggag ggccgactcg tccgcgggac
      781 taaacggcat cccaccatcg aggatcgcgt tgtgatctac gccaatgcca cggtcttggg
      841 gggacgcact gtggttggac acgattcggt gatcgggtcg agtgtctggt tgacccggtc
      901 tgtgccgccc cattcgacgg tcacgttgga acgcccaaac ctgcggatct ttggggaacg
      961 ttgtgaggac ctcaagcccg aggccagtct cgctgatgct ccgcgcacgt cggccaaggc
     1021 cacagcgggg ggcaacgggg cagcgcccgg cacgaccggc cattaatact ccggggctgt
     1081 ccggattagt caacgacttc cttggcgttg acataagagg cggcggctta tccgcatgag
     1141 gtaggggaga actaccatca aggcgattcc gagccaagcc acggcggaga gggccatccc
     1201 taagaaaaac cccgaatcgt aataggtcca ggtgatttgc cattttcccg gcggaagggc
     1261 aacacccatc atgattcggt tgatccgcaa tggacgggca tcccacgagg tggaaccatc
     1321 ctgcgaaacc acgtgaaccc gccagccggg catgaactgc tcgcgtaaga caacaattcc
     1381 cggctcttga agagaggcgt agatgaccac cttgccaggt tgaaatgctt tgacgtgaca
     1441 atcctccttg gtcgttcttt cttgagtttg tgcggccttg ggagggatct gctcgagaat
     1501 cgccaagcca cacggttcaa tggattccac tacgaaggtg tctaaaggac cacgagggcg
     1561 tccgttggga aaaaagaccc attctgtttt ctgccagaca ttcaggcgag cagacagttc
     1621 ggaaggaaac ttcgtgacgt tgtgtgtcag aaaacaatag tgactttcac gagccgtcgg
     1681 cgagtctggg cgacttgttt tctcttgttg gttcgactcg agaaaggcgt cggttgtcaa
     1741 agcgccaaga agtgcgagtg tttcctgagg cgggatcttc aacttctggc gattcatgta
     1801 ctcatgaagg atcgccagga atacgaaata atcaccggga acaagtgttc cgtaactctc
     1861 caagggggcg cgccccgtca acatgcccca gcgaatggcc gatgtttcgc gctcccaatc
     1921 gaaaagctcc tgaatgcggg tacaagacgg ggtttgccac tcccgtgggt agtcacacca
     1981 tttctgccaa gatcgaaatg gcggaagaga atgcaacgcc atttgcgttt ccttgcccac
     2041 atgtacgggg aagatcatcc aactgttgtg gaggacgagg tccagtcccg taaggagtac
     2101 gatgacgatt ggcaaatgac ggattggcac ccacctcacc aaacacagcc aggtgaggag
     2161 aagggccagc aggaggacca aacctgtgac ggtctcccat cttgcccttc cgggttcgaa
     2221 aaaaccaaac accggatctt gcggaacgta ttgcgaggcg tactcccaca aatgggaggc
     2281 aaagagggcg acaagaagaa aacacgtggc ccaggcaagg atcagtatca aaagacgcgt
     2341 cagccgtcgc aggtacttcg tgtcaagatg gtcgatcgca agtgaagcca ggacggaaag
     2401 accaagcgtt gtcaggacga gccatttgga cgggtaacgg aataacccgt acccaggcaa
     2461 aatctgagtg actacccaat acagcccacc gacaggaggc gaaaccgtcc cgttactact
     2521 atgacccgtg atccatgccc acagctcgcc atagacgccc gcgggaccat accagcccaa
     2581 ggcagccacc aggctgaaca cagtgaccca ggaaaaccaa atgcggaacg cctcgggagg
     2641 catattcggc gaattgcgct gtgacgattc gcaatatctt gcgtccgaac ctgttgacga
     2701 gaatgatctc cgcgctggcc aaaaccgcat ggtgaggatc gcgagcaaaa agggcaatag
     2761 tcccatgtac agagacggtg tccaccagcg gccttccgaa acaatcgctc cgatccatcg
     2821 cgtattccag ggtagggggc gaccaccgaa ctctggccag acaaattcga tccaacgata
     2881 ggccggcgtg ctaaaatcgt agataactcg ttgccactgg gacacttgcc ggctacgggc
     2941 gatctctgca agtgtgtttt gctctgtgct tttggcagtc ttgtcgtcct gacgttccgt
     3001 gagacgattt cccgaatcac tagccgcggg cttgagaagg gcccgttcgc taaggcgggt
     3061 gtaggtccac gtcggaataa tctggattgc cgcgagtaga agagtgacca acgccgcgcc
     3121 gattagcagg aggccgcgcc ttaagaccct tgccaacggc tggcggttcc tttccccgtt
     3181 ggaacggcgt ccgagtgcac tgaaaataag aacaatccag gttgcgaagg caagcacggc
     3241 gcaatggtaa gcggtgtgtg gatcgccccc taggatcatg agggctagaa aaacacccaa
     3301 tcgaatggct gatcgagctt ggaccggggg caaaaagtgg agccatgagt ggattgtatt
     3361 tcctttcgtg gcgtggtcgt tatccgttga ttgccgggtg agtctgttcc tctcaaaaaa
     3421 tatgtcaaac agggcatgga tggcccaagg gagccaggcc gcgctaatca aaaaaatcgg
     3481 gttgtatatt tggaacagga cgtaacctga aagcggatat gatagcgttg taagggaagc
     3541 cgccacgggg gtggcaccac accgccgagc caaccgcgca atgccccacg cagctaggca
     3601 aagatggacg atgagaaaaa ccaggtaagc ggccgaaaaa gggatcggca acattagaat
     3661 cacaattccc ggataaaagg cggcacaact gttctccgcc gctagaggag caccgaaatt
     3721 aagataggga ttccacaacg gcaaatgacc ttggcgg
//
LOCUS       DAOPTR010000290         3755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19591_length_3755_cov_3.502703, whole genome shotgun sequence.
ACCESSION   DAOPTR010000290 DAOPTR010000000
VERSION     DAOPTR010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19591_length_3755_cov_3.502703"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="PKI05_06680"
     CDS             complement(<1..665)
                     /locus_tag="PKI05_06680"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA51924.1"
                     /translation="MSRCVNVCALVTCAMVASFVLNRSSGAEEGQIRKLGLIGLDTSH
                     VIAFTDIINAKDAEGVLAEFEVVAGYPNGMPDNPSSWNRVEEYTSKLRDKGIKIFDTI
                     EEMLPHVDAVLIESVDGRPHLEQARKVISARKPIFIDKPMAASLCDVLLIFRLAEEAK
                     VPVFSSSSLRFSSGFQAVRKGEAGFGTVKRCVAWSPLHIEPHHPDLFWYGIHGVETLY
                     TIMG"
     gene            complement(724..1683)
                     /locus_tag="PKI05_06685"
     CDS             complement(724..1683)
                     /locus_tag="PKI05_06685"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOA51925.1"
                     /translation="MSLNATMTRRRFVETSSALVAGLSWRVPAWGAETSATIKKAMIG
                     KPTEETLKQLKAAGFHGIESNDRHADLKDAESARKLAESLGMRIHSVLYGWANFNTDS
                     VNSDIESVARSLETAAAYGADALLLVPCRIGGMPMPEAWEFDIEFDEKTGHVLRVVKG
                     DNSPYRTYIEAHNHAIDTSKEAVKKLIPVAEKTKVIIALENVWNNLWVKPAIFAHFVK
                     SFDHPMVQAYFDIGNHVKYAPPVEWIRALGKTIVKLHVKDFLLNPDGRGGKFVHPRDG
                     SVDWPAVRAELNKIGYTGWMTIEDSGLSLEEFSQRLDMIIAGK"
     gene            complement(1789..>3755)
                     /locus_tag="PKI05_06690"
     CDS             complement(1789..>3755)
                     /locus_tag="PKI05_06690"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOA51926.1"
                     /translation="GEGGIIEEVRGDVFGYSERLLRLVRNQPHLDLFLNTHATGVEME
                     SPKRIKAVIALDVNTGERYRFPAKTFIDCTGDGVIGVWAGAEYRHGPEPRSMYNESRA
                     PEQGEVNKGTMGGTLRYMTEQLAQPVEFKAPDWAHKFHDCSDFNPGRHPQLQFGGWQW
                     VIEYGGELDTYHDAEVIRDELLRIIWGMWDHAKNHCPKLKEEAKNCKLTWVSYVVGKR
                     ESRRLIGDYIMTEHDIANQTLFPDRVAYGGWGIDIHPPKGFYDPGPPAIFSHKVKFSI
                     PFRSLYTKDVDNLMMAGRCISVTHAALGATRVMITCGLQGQATGTAAGFCKMHDTTPR
                     GVYERYITDLQQQLLKDGCYIIDLPNQDPRDLARSAKVSASSVAPNEVYSVASFLPVH
                     KLDHDRAVMFTWQGGRLDAIKVYLNSERSDSVRLTGHLCVATGLGEFNGAEEIAQIEA
                     EVLPGKREWVSFGVNRDLPKGYYYLWLPKTSGVSWSLFAQRPAGTSRAYRGAGAWTPM
                     PDCYAFQLVPGETPQLPLAGEDSIAYFAPENVIDGFARAIRGRPHSWRPDPKLAWPQW
                     VALEFPQSVSFNTVHVSFQSASMRADDFDIEVWRNDQWVRIVEVRGNTERRRVLTFPR
                     VTAQKVRLVIHRAQPDMGVCEIRVYDESEQ"
BASE COUNT          867 a         1092 c          952 g          844 t
ORIGIN      
        1 cccatgatcg tgtaaagggt ttccacgccg tggatgccat accagaagag atctggatgg
       61 tgcggctcga tgtgcaacgg gctccaggcc acgcagcgtt tgaccgttcc gaagcccgct
      121 tcgcctttgc gaactgcttg gaaacctgag ctaaatcgta aggaggaact ggaaaacaca
      181 ggaaccttgg cttcctctgc cagccggaag atcagcaata cgtcgcaaag ggatgccgcc
      241 ataggtttgt cgatgaagat gggcttgcgg gcggagatga cttttcgcgc ctgctccagg
      301 tggggacgac catcaacact ttcgatcagg accgcgtcta catgcggcaa catttcttca
      361 atcgtgtcaa atattttgat gcctttgtcg cgaagcttgc tcgtgtactc ctcaacacga
      421 ttccaactcg aggggttatc aggcatacca ttggggtaac cggccacaac ttcgaactct
      481 gcgagcacgc cctcggcgtc cttggcgttg atgatgtccg taaaagcgat gacgtgcgag
      541 gtatcaagcc caataagccc taatttccga atctgaccct cctccgcacc tgaggatcgg
      601 ttgagcacga aagacgcaac cattgcgcac gtgaccaatg cgcaaacatt tacgcaccga
      661 gacatcgttg tatctccttt aagcacaaca aacggtgttc attcacacag tgcgaagtct
      721 atgttatttc cccgcgataa tcatgtctaa gcgttgacta aactcttcca gggaaagacc
      781 actatcctcg atggtcatcc agcccgtgta gccgatcttg ttcaattccg cccgcacggc
      841 cggccaatca acgcttccat cccgcgggtg gacaaatttg ccgccacgac catcgggatt
      901 caacaggaaa tctttcacat gcaacttgac aatagtcttt cccagtgcgc gaatccactc
      961 cacgggcgga gcatacttca catggttacc gatgtcgaaa tatgcctgaa ccatggggtg
     1021 gtcaaacgac ttgacaaagt gggcaaaaat cgcgggtttc acccacaggt tgttccaaac
     1081 attttccaag gcgatgataa ccttggtttt ctcagcgaca ggaatgagct ttttcactgc
     1141 ctccttcgag gtgtcaattg cgtggttgtg ggcctcgatg taggtacgat agggactgtt
     1201 gtcacctttg acaacgcgaa ggacgtgccc cgtcttctca tcaaactcga tgtcgaactc
     1261 ccaagcctcc ggcatcggca ttccgccaat gcgacaagga accagaagaa gggcatccgc
     1321 cccataggcg gcagcggtct ccaaagagcg ggccacgctt tcgatatcgg aattcacact
     1381 gtccgtgttg aagttggccc agccgtagag gaccgaatga atcctcatgc caagcgactc
     1441 ggccagtttg cgggcagatt cggcatcttt cagatcggca tgtcgatcat tcgattcgat
     1501 cccgtgaaac cccgccgctt ttaactgctt gagggtttcc tcggtgggct tgccaatcat
     1561 ggcctttttg atggttgcag aagtttccgc accccacgct ggcacccgcc aggaaagtcc
     1621 ggctaccaag gccgaagacg tttcaacaaa acgacgacgc gtcattgtgg cgttaaggct
     1681 catagctttt ctcccttttt gcaaacgcca aaggttttct aaacccgaat aggcaagacg
     1741 gctgcccaga atttacgttt cacaagccct cagccagtgt tcacatgttc attgttccga
     1801 ttcatcataa acgcgaattt cacacactcc catgtcaggt tgtgcccgat gaatgaccag
     1861 ccgcaccttt tgtgcggtca ccctcgggaa ggtcaaaacc cgacggcgtt cggtgttacc
     1921 ccgaacctcg acgatgcgca cccattggtc gtttctccag acctctatat cgaagtcatc
     1981 agcccgcatg ctcgctgact ggaacgacac atgtaccgta ttgaacgaaa ccgactgcgg
     2041 gaattccagc gcaacccatt gaggccaggc gagcttagga tcaggccgcc atgaatgtgg
     2101 cctacccctg attgcccgcg cgaaaccatc gatcacgttc tccggagcga aataggcgat
     2161 cgagtcctcg ccggcgaggg gcaactgcgg tgtttcgcct gggacaagct ggaaggcata
     2221 gcagtctggc ataggtgtcc atgcgccggc cccgcgataa gcccgagatg tgcccgccgg
     2281 acgttgtgca aagagactcc acgagacccc ggaagtctta ggtagccaca ggtagtaata
     2341 acccttggga agatcacgat tcacaccaaa ggagacccac tccctcttcc ccggcaagac
     2401 ctctgcctcg atctgggcaa tctcctcggc accattgaac tcgcctaagc cagttgccac
     2461 gcagaggtgt cccgtaagcc gaacactgtc agatcgctcg gagttcagat acaccttgat
     2521 cgcatccagt cgtccgccct gccaggtgaa catcaccgca cggtcgtgat cgagtttatg
     2581 aacgggcaag aagctcgcca ccgaatagac ctcgttggga gccacgctgg aggcggaaac
     2641 ctttgccgag cgagccaagt cacgcggatc ttggttagga agatcgatga tataacatcc
     2701 atctttcaaa agttgctgct gcaaatcggt gatatagcgt tcgtaaacgc cacgtggggt
     2761 tgtatcgtgc atcttgcaaa aaccggcagc cgtccccgtg gcctgacctt gaagaccgca
     2821 ggtaatcatc acgcgggtgg cccctagcgc tgcatgcgtt accgaaatgc accgcccggc
     2881 catcatgagg ttgtcaacat ccttcgtata aagactccga aatggaatag aaaacttgac
     2941 tttatgggag aagatcgccg gtggccccgg atcatagaaa cctttggggg gatggatatc
     3001 aatcccccac cccccataag caacgcggtc cgggaagagc gtttgattgg cgatgtcgtg
     3061 ctccgtcatt atgtaatcgc cgataagacg tcgcgattcc cgttttccga ccacataact
     3121 cacccaggtt aacttgcaat tcttagcttc ctccttcagc ttggggcagt ggtttttggc
     3181 atgatcccac ataccccaga tgatgcggag aagctcgtcg cgaatgacct ccgcatcatg
     3241 atatgtgtcc aattccccac cgtattcgat cacccattgc cacccgccaa actgcaattg
     3301 gggatgcctt cccggattga aatcggagca atcgtggaac ttatgcgccc aatcaggggc
     3361 tttgaattcg actggctggg ccagctgttc ggtcatgtag cgaagtgttc cccccatcgt
     3421 acctttattg acctcacctt gttctggcgc ccgcgactcg ttgtacattg agcgcggctc
     3481 tgggccgtga cggtactccg cccccgccca aacaccgatc accccgtctc cggtgcagtc
     3541 gatgaaggtt ttggccggaa aacgatagcg ctcgcctgtg ttaacgtcta gggcgatcac
     3601 cgctttgatc cgtttgggcg attccatttc caccccggtg gcatgagtgt tcaagaaaag
     3661 atccaagtgt ggttgattcc tcactagccg gaggagacgc tctgaatagc cgaagacatc
     3721 tccacggact tcctcgataa taccgccctc cccgg
//
LOCUS       DAOPTR010000291         3744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19678_length_3744_cov_3.035782, whole genome shotgun sequence.
ACCESSION   DAOPTR010000291 DAOPTR010000000
VERSION     DAOPTR010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19678_length_3744_cov_3.035782"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /gene="pth"
                     /locus_tag="PKI05_06695"
     CDS             <1..480
                     /gene="pth"
                     /locus_tag="PKI05_06695"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOA51927.1"
                     /translation="RRWAAEVRPRRQFSGETVDATINGIKVLLLSPTTYMNRSGTSLR
                     AACDFYKLKPQDVLVICDDMNLPLGRLRVRPRGSSGGHKGLADIIRAMGTEEIPRLRV
                     GIGKPPPGQDSVRFVLSVFGKEEEAVIREAYRKAADAVETWVTEGIDVCMTRFNGGN"
     gene            562..993
                     /gene="rpsF"
                     /locus_tag="PKI05_06700"
     CDS             562..993
                     /gene="rpsF"
                     /locus_tag="PKI05_06700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOA51928.1"
                     /translation="MAQNFYEGLYILDSNRFARDPDGVVQQITDALTGCGAEILVSRL
                     WEERRLAYPIKKQRRGTYWLIYFKMESTRLGEVNRRLQLVSDILRFMHIKIDPRIIDV
                     LVEHARGGLAMTRPSADVTVDVEDLDEEIDSEVEDLDAVEE"
     gene            1083..1526
                     /locus_tag="PKI05_06705"
     CDS             1083..1526
                     /locus_tag="PKI05_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOA51929.1"
                     /translation="MASFNRVILVGNLTRDPNVRYTPSGTAVCEIGLAVNERRKNQSG
                     EWVDEVVYVDVTLWGRTAEVAGEYLTKGSPLLIEGRLRMDTWEKDGEKRSKLRVVAER
                     MQMLGTRGAQNDSGARPGGTKPPVPGPVEGVLDETGFPPEDDVPF"
     gene            1595..2146
                     /gene="rplI"
                     /locus_tag="PKI05_06710"
     CDS             1595..2146
                     /gene="rplI"
                     /locus_tag="PKI05_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOA51930.1"
                     /translation="MMATGKGTKKNRKHGLRGLPRGPHGGVQLLLVQNVEHLGKAGDV
                     VEVKRGYAVNYLIPQGLATIATEHHKRMVEKHKAKLLEIERARISTMKQLAENISQMS
                     ISIEANANEEGHLYGSVGADDISRALKAQNININPDQVKLQGPLKELGLYTVQIHLGH
                     DIYADLKVWVVPMVGDTRPGVSR"
     gene            2426..3640
                     /locus_tag="PKI05_06715"
     CDS             2426..3640
                     /locus_tag="PKI05_06715"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOA51931.1"
                     /translation="MILQAKKKMAISVLWAVLILCSTPVLLAEDERCLPFVSPAQVGM
                     KAEVLAKIPVRLQEFVEAREIAGAVTLVARNGSIVHWEAVGWADIEKQIPMRKDTLFQ
                     IASMTKPITATALMVLVDRGKVALDDPVSRYLPEFKEITVKGQRLTRPLTIKHLLTHT
                     SGLTGSQRTEATLQATVEAIVKGNLAFQPGERWNYSPGLSVCGRIIEVVSGMPYEQFL
                     EKEIFQPLEMVDTTFVPNEHQRERLAKAYQRTADGQLLPGGGASWLIEDAGVRAPNPS
                     GGLFSTAGDLVRFYQMILNGGELCGRRILSPEAVRLMTTVQTDDLQTGFTPGNGGGLG
                     WCIVRQPQGVTEMLSPGTFGHGGAFGTQGWIDPQRQMIFILLVQRTNFPNADASAIRE
                     AFQRLAVEALEL"
BASE COUNT          845 a          941 c         1142 g          816 t
ORIGIN      
        1 cgccgctggg ccgcagaagt acgaccgcgt cggcagtttt ccggcgaaac ggttgatgct
       61 acgataaatg ggatcaaggt gcttttgctc agccccacga cgtatatgaa ccgaagtggg
      121 acgagcctgc gggcggcttg tgatttttat aagctcaagc cgcaagatgt cttggtgatt
      181 tgtgacgata tgaaccttcc gttgggaagg ctacgggtgc gaccgcgagg gtcctccggg
      241 ggacacaaag gtttggcgga tatcatccgc gcgatgggaa cggaagagat tccgcgacta
      301 cgcgtcggca tcggcaagcc gcctcccgga caggattccg ttcggttcgt cctttccgta
      361 ttcggaaagg aggaagaggc cgtcatccgt gaggcctatc gaaaggcggc ggatgccgtg
      421 gaaacatggg tcaccgaagg aatagacgtt tgcatgacac ggttcaacgg tgggaattga
      481 gcttctgagt tggaaataaa aaaagcgctg tcctggcaag atacaaaaat cgaagcggta
      541 tttggtggaa ggagcgttca tttggcacag aacttttacg aaggcctgta tatcctcgat
      601 tccaacaggt ttgcccgtga tcctgacggg gttgtccaac agattacgga cgcacttacc
      661 ggctgcggcg cagaaatcct ggtgagccgg ctgtgggaag agcggcgctt ggcttacccg
      721 atcaagaaac agcgccgtgg gacgtattgg ttgatctact tcaagatgga aagcactcgc
      781 ttgggggagg ttaatcgccg cctgcaactg gtttcagata tcttacggtt catgcatatc
      841 aagattgatc cacggattat cgatgtgctg gtggaacacg cccgcggtgg cttagcaatg
      901 acacgtccgt cggctgatgt gacagtggat gttgaggatt tggacgagga aattgattcc
      961 gaggtcgaag acctagatgc tgttgaggag tgatcttact tctctgacaa tccgcggcat
     1021 atgcggcgga ttttgtgtgg ggaagtgggc accctggcga ttcgagtgag gggggaacgg
     1081 ccatggcgag cttcaatcgc gtcattttgg tgggaaactt gacacgcgac cctaatgttc
     1141 gatacacgcc cagcggcacc gccgtttgcg aaattggttt ggccgtcaat gaacggcgaa
     1201 aaaaccagag tggcgaatgg gtcgatgagg tcgtttacgt ggatgtgaca ctctggggac
     1261 ggactgcgga agtcgctggt gaatatctca ccaaaggctc gccactactg attgaggggc
     1321 gactccgcat ggacacgtgg gaaaaagacg gcgagaaacg gtccaaattg cgggtcgtag
     1381 ccgagagaat gcaaatgctg ggaacccggg gggcgcagaa cgacagcggg gcgcggcctg
     1441 gcggcactaa gcccccggtg ccaggcccgg ttgagggtgt cttggatgag acaggttttc
     1501 cgccggagga tgatgtgcca ttttgatgtt tctccgcgcg atatgagcct atagcctaag
     1561 cctaagagag tctttattct tcttgagagg atcgatgatg gcgaccggaa aaggaacgaa
     1621 gaaaaatcgt aagcatggcc ttcgtggcct tcctcgaggg ccccatggtg gtgtccagct
     1681 ccttctcgtc cagaatgtag agcacttagg taaggcgggg gatgtcgtcg aagtcaagcg
     1741 cgggtacgcc gttaattatt tgatcccgca aggtctcgcc accatcgcga ctgagcacca
     1801 caaacgcatg gtggaaaaac acaaggccaa attgcttgag atcgaacgtg cccgcatctc
     1861 cacgatgaag caattggccg agaatatctc gcagatgagc atctccatcg aggctaatgc
     1921 caacgaggaa ggacaccttt acgggtcggt cggggcggac gacatttccc gggcccttaa
     1981 agcccagaat atcaacatca accccgacca ggttaagctt cagggaccgc tcaaggaact
     2041 tgggctttat acggtccaga tacacctggg acacgacatc tatgcggatc tcaaggtttg
     2101 ggtggttccc atggtaggcg acacccgtcc cggtgtgtcc cggtgaaatt tttcattgcg
     2161 gcacctgatt cattggttag agtattccgc ccgtcacaac gacgggcttt tttgtgcgca
     2221 aatttggcct aaacacgcct gcaaagccct ccccgggtct tgcattctcg gtaccgcatg
     2281 ctaagttgtg gactgtcgat ggacgaggtt catgtggagc ttcgctcggc gagatcctga
     2341 aggtgcagcg ctaacgcgca cttccccccg cggagtgccg tatctgacaa gaccccaaga
     2401 cggcgaaaga aacaaggact ttgacatgat cctacaagcc aaaaagaaaa tggcaatctc
     2461 agtgctatgg gcggtcctca tcctttgcag tactcccgtg cttcttgcgg aagatgaaag
     2521 atgtcttccc tttgtctcgc cggcacaagt cggcatgaag gccgaggtcc tcgccaaaat
     2581 tcccgtccgt ttgcaggagt ttgtcgaggc gcgcgagatc gcaggggccg ttacccttgt
     2641 tgcgcggaat ggctccattg tccattggga agcagtgggt tgggcggata tcgagaagca
     2701 gattcctatg cgaaaagata ctcttttcca aatcgcctcg atgaccaagc caatcacggc
     2761 gaccgctctt atggtattgg tagatcgggg gaaggtggcc ttggatgatc ctgtttcgcg
     2821 gtatttgccc gagttcaagg aaattaccgt caaggggcag cggttaacaa gacctctgac
     2881 gatcaagcat ctgctaaccc acacctcggg actcacagga agccaacgaa cggaggcgac
     2941 gcttcaggcc acggtggagg cgattgtcaa agggaacctt gcttttcaac cgggggagcg
     3001 atggaactat agtcccggtc tgagtgtgtg cgggcggatt atcgaggtcg tctccggaat
     3061 gccctacgag caattcttgg aaaaagaaat ctttcagccc ttggaaatgg tagataccac
     3121 gttcgttccc aatgaacatc agcgtgagcg actggccaag gcctatcaaa ggacggcgga
     3181 tggtcaactt ctgccaggtg gcggcgcctc ttggctcatc gaggacgcag gcgttcgtgc
     3241 ccccaacccg tctggggggc tgttctcaac ggcaggtgac ttggtgcgtt tttaccaaat
     3301 gatccttaac ggtggggaac tgtgtggtcg gcggattctc tcgcccgaag cggtacggct
     3361 tatgacgacg gttcaaaccg atgacttgca gacgggtttt acccctggca acggcggggg
     3421 attgggctgg tgtattgtgc ggcagcctca aggggtcaca gaaatgcttt cgcccggcac
     3481 gttcggtcat ggcggggcct ttgggacgca gggatggatc gacccgcagc ggcagatgat
     3541 ctttatcttg ctcgtccagc gaaccaattt tcccaacgct gatgcttcgg ccatcagaga
     3601 agcctttcag cgcctcgcag tagaggcact agagctttga cccgtggcac ctgcaccgaa
     3661 cgcaactaag agtctatccc acaacctctc ctcgcccgtt cggcgggaga ggggccgggg
     3721 gtgagggtgg ctcgaatcgc tgcc
//
LOCUS       DAOPTR010000292         3738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19715_length_3738_cov_3.581320, whole genome shotgun sequence.
ACCESSION   DAOPTR010000292 DAOPTR010000000
VERSION     DAOPTR010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19715_length_3738_cov_3.581320"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="PKI05_06720"
     CDS             complement(<1..831)
                     /locus_tag="PKI05_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51932.1"
                     /translation="MRSRAYACGLWLSVALGLIALQKQVVAQFESMPLAGAWRFQIDR
                     DNVGIEQKWWTNRLLDEIQLPGLLQAQGYGDPPGPQSQWLAGIGLKRADDPLFAPYFK
                     GKEFLSPFFLTPPRHYVGAAWYQRDVVIPETWKDCHITLTLERVHWESRVWIDDREVG
                     RQNSLAVPHVYDVSKYLSPGKHQITIRVDNSYLIPVGKSAHSVSDETQGNWNGIVGRM
                     ELQATPPVWIEDVQVFPNAKERLVDVKVTIGNMTGRAGQGQLRLSAHTLSREAVSPSE
                     R"
     gene            1092..1499
                     /gene="gcvH"
                     /locus_tag="PKI05_06725"
     CDS             1092..1499
                     /gene="gcvH"
                     /locus_tag="PKI05_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOA51933.1"
                     /translation="MNPDELKYTKTHEWVAIEKTSDGKTLAVVGITDFAVQSLADLVF
                     IELPPVGKEVKAGEPLGQVESVKAVSDIYSPVTGRVVDANSHVAEHLEDLTADPFGRG
                     WLAKLEVTDPTSLSGLLDRASYEAQCAAEAEEH"
     gene            1731..3092
                     /gene="gcvPA"
                     /locus_tag="PKI05_06730"
     CDS             1731..3092
                     /gene="gcvPA"
                     /locus_tag="PKI05_06730"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOA51934.1"
                     /translation="MAYLPQTEDEVRAMLAAIGVPDIDSLFAPIPSDVRLNRPLQLPP
                     ALTELELTQHLEEVASQNQGTHQFTCFLGGGAYDHFIPAVVDFVASRSEFYTSYTPYQ
                     PEVSQGNLQVFFEYQTLITQLTGMDVSNASLYDGASALAEAVLMALGSQPGRRRVIVP
                     ENVHPEYRTVLATYLRHTSAEIVLVPTPKGIVEPEALSQALDAGSVACVILQQPNFFG
                     CVESAGRISEITHQAGAILIAVVDPISLGMLRRPGDYGADIVVAEGQPLGLPMAWGGP
                     YLGILACRETFLRRLPGRLVGQTVDRRGNRCWVLTLQTREQHIRREKATSNICTNQGL
                     MALRATVYLAAMGPGGLRRVAEACLQKAHRLYRQLIEKTGLKPLFSAPFFKEFALQAN
                     SADEVVELLEGAQHEGFFAGLPLGRWYPQYADALLIAVTEKRTEQEMDHLVETLSNLR
                     RRG"
     gene            3145..>3738
                     /locus_tag="PKI05_06735"
     CDS             3145..>3738
                     /locus_tag="PKI05_06735"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121465.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOA51935.1"
                     /translation="MRNRQATQLIFELSQPGTGEEYIPAVNFTEDDIRSFLPAEELAT
                     ELPPLPEVSEPVLIRHFINLSQQNMSVDTHCYPLGSCTMKYNPKRNDRLAQLPGFQNI
                     HPYQPVETIQGLLKVLYSLQEMLAEIAGMDAVTLQPAAGAHGELTALLIAAAYFRHRG
                     EHRNTVLIPDVAHGTNPASAAMAGFRTVEVKSNAHGLV"
BASE COUNT          805 a         1057 c         1049 g          827 t
ORIGIN      
        1 cctttcagaa ggagaaaccg cctcccggct cagagtgtgc gcactcagcc gcaactggcc
       61 ttgcccggcg cgacccgtca tgttgccaat agtgactttc acgtccacca aacgttcctt
      121 cgcattggga aagacttgca cgtcttcaat ccacacaggc ggtgttgcct gcagctccat
      181 cctgccgacg atgccgttcc agtttccctg ggtctcatcc gagacactgt gggcgctctt
      241 tcccacggga ataaggtagc tattgtccac gcgaatcgtg atctggtgct tccccggcga
      301 caagtatttc gacacgtcat agacgtgcgg tacggcaagg gaattctgac ggccgacttc
      361 tcgatcatca atccaaacgc ggctttccca gtggacgcgc tccagcgtga gggtaatgtg
      421 gcaatctttc caggtttcgg gaattaccac gtcgcgctgg taccatgccg cgcccacata
      481 atggcgcggt ggtgtcaaaa agaagggcga gagaaattct ttccctttga agtatggggc
      541 aaagagggga tcgtcggccc gcttcaaacc gattcccgcc agccactgac tttgtggacc
      601 cggcggatcg ccgtagccct gagcttgtaa aagccctggg agctgaatct catctagtag
      661 gcgattggtc caccactttt gctcaatacc gacgttatcc cggtcaatct gaaagcgcca
      721 tgcgcccgct aagggcatgg attcaaattg ggcaacgacc tgtttttgca gggcgataag
      781 cccaagcgcg acagaaagcc acaaaccaca tgcataagct cggctccgca tccttaagtc
      841 tcctcacgat gaaccacgtt ctacgaatcg ccaagagtta tgcaatctaa tcaaatcgtt
      901 aggaaaaagc caccatccgg caagaatgtc ccgtgacaat ccgggggaac tactgtgaca
      961 atcatctgtg ttataaaatg cattattccg atttgccggg cttgcatggt gttctcaagt
     1021 tgccaattgc gagcggcact cggaggctat cttcaccagg gcaagatttt ccaatcgagt
     1081 gggggtttcg cgtgaatccg gacgaattga agtacaccaa gacacacgaa tgggtcgcca
     1141 ttgagaaaac atccgatggc aagacgctcg cggtggtggg gatcaccgat ttcgccgtgc
     1201 aatctttggc ggacctcgtc ttcatcgagc tacctccggt gggcaaggag gttaaggcgg
     1261 gggagccact cggtcaggtc gaatcggtca aggccgtcag cgatatttac agccctgtga
     1321 ccgggcgcgt cgttgatgcc aattcccacg ttgcggagca cctcgaggac cttacggcgg
     1381 atccgtttgg acgcggttgg ctggccaaac tcgaggtcac cgatccgaca agcctatcag
     1441 gccttcttga ccgggccagt tatgaggccc aatgtgcggc cgaggccgaa gaacactaag
     1501 cgtggtcacg tgcgggcaga acatcttgcc ggacttgcga gggttttcca aggctttgat
     1561 tttttcgggg cacgatgggg aatacaaagg gcgggtatta cgcatggctc ggcgaccaag
     1621 gtggtcggtg agtatgattc tttgacaaat gatgttcggt tgttggccga tcgttatctc
     1681 gcggcagatg gcgatggccg tcgctgccgt ctttttaggg ttaatgagac atggcgtatt
     1741 taccgcagac cgaggatgaa gttcgggcaa tgcttgcggc aattggcgtg cccgatatcg
     1801 acagcctctt cgctcctatc ccgtccgatg tgcgacttaa tcgcccgttg cagcttccac
     1861 cggcgctcac tgagctggaa ttgacgcaac acttggagga agttgcctcc cagaatcaag
     1921 ggacgcatca attcacgtgt tttctgggag gtggagctta tgaccacttt atcccggccg
     1981 tggtcgattt tgtggcctcg cggagcgaat tctacacgtc ttataccccc tatcaaccgg
     2041 aggtcagtca aggtaacctt caggtctttt tcgaatacca aacactgatc actcaactga
     2101 cggggatgga cgtttccaat gccagcctct atgacggggc cagcgccttg gccgaagccg
     2161 tgctcatggc ccttgggagc cagccgggac gacggcgggt gatcgtcccc gaaaatgtcc
     2221 atcccgaata ccgcacagtc ctcgcgacgt acctgcgtca cacgtcggcc gagatcgtcc
     2281 tcgtgccgac gccaaaggga atcgtggaac cggaagccct ttcccaggcg ttggacgcgg
     2341 gctcggtggc atgcgttatc ctgcagcagc cgaacttctt tggctgcgtg gaatcggcag
     2401 gtcgcatttc cgagatcacg catcaagcgg gggccatcct gatcgctgtg gttgatccga
     2461 tcagcttggg catgcttcgc cgaccaggcg actacggggc cgacattgtg gtggccgaag
     2521 gacagccact cggtttgccc atggcatggg gaggacccta tttaggaatt ctcgcatgtc
     2581 gcgaaacatt tctccgccgc ctgcccggac ggcttgtcgg ccaaacggtt gatcgccgcg
     2641 gcaatcgctg ttgggtcctc actctacaaa ctcgcgaaca gcatatccgc cgagaaaagg
     2701 cgacaagtaa catctgcacc aatcaagggc tgatggccct gcgggcaacc gtgtatttgg
     2761 cggccatggg gcctggcggt cttcggcgtg tggccgaggc ctgtttgcaa aaggcccatc
     2821 gattgtatcg ccaattaatc gagaagacgg ggctcaagcc gttgttttcg gcaccctttt
     2881 tcaaggagtt cgccctgcag gccaattccg ccgacgaggt tgttgaactt cttgaagggg
     2941 cgcaacatga aggattcttt gccggtctgc ccctgggacg gtggtatcct caatatgcag
     3001 acgccttgct catcgccgtc accgaaaaac gcaccgaaca ggaaatggat cacctggtcg
     3061 aaactctgag taatctgcgg aggagaggct gacaccgaaa gcgacgcgat gttgtcatcc
     3121 attgtatagt tgtgcaggtc aatcatgcga aaccgacaag cgacgcaact gatcttcgag
     3181 ctttctcagc ccggaacggg cgaagagtac atccccgcag ttaatttcac cgaggatgat
     3241 atccgatcat tcctcccggc cgaggagttg gctaccgagt tgccccccct gcccgaagtc
     3301 agcgaacctg tgctcattcg gcatttcatc aatctttccc agcagaacat gtcggtcgat
     3361 acgcattgtt atccgctggg ctcctgtact atgaagtaca accccaagcg caacgaccga
     3421 ctagcccagt tgccgggttt tcagaatatt catccttatc agccggtcga aactatccag
     3481 ggcctcttga aggtcctgta cagtcttcag gaaatgcttg ccgaaattgc cgggatggac
     3541 gcggtaacgc ttcaaccggc agcgggggcc catggggaac ttaccgccct gttgatcgcg
     3601 gcggcctatt tccggcaccg tggcgaacac cgcaatacag tcctgatccc tgatgtggcc
     3661 cacggtacca atcccgccag tgctgcgatg gccggatttc gcaccgtgga ggtcaaaagc
     3721 aacgctcatg ggttggtc
//
LOCUS       DAOPTR010000293         3738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19716_length_3738_cov_3.081998, whole genome shotgun sequence.
ACCESSION   DAOPTR010000293 DAOPTR010000000
VERSION     DAOPTR010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19716_length_3738_cov_3.081998"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1587)
                     /locus_tag="PKI05_06740"
     CDS             complement(<1..1587)
                     /locus_tag="PKI05_06740"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOA51936.1"
                     /translation="MAGLVFLSIVVFLPAFGALVLMFFPSHAERAIRRTTLGVTLVVF
                     LLTLRLVLPGLHAASQNPLEQVLHDVRFAAGEPGYQHAYAVPWISSFHIDYFLGVDGI
                     SLPLVLLTSFLSVLAAAASWSVTKHVKAYSILFLLLETGMLGVFLALDFFLFYVFWEV
                     MLLPMYFLIGVWGGPRREYAAIKFFLFTLVGSVLMLIAILLLYFASDVRQLPENARRE
                     SFLSQEVMVKSLAPSEKPLHTFNLIALAKLGQMPDSPFQEPVLGGKSVAWWAFLLLLI
                     GFLIKVPAVPVHTWLPDAHVEAPTPISMILAGILLKMGGYGIIRICYPICPQGGYELA
                     WFVCLLGIVSMVYGALAAMAQQDFKRLVAYSSVSHMGYVVLGLGAWSLMPGLYDPQYW
                     KMGMNGAMFQMIAHGISSAGMFFVVGVLYDRVHHRDLNQFGGIFGRMPVYTALSIVIF
                     FAALGLPGLCGFVGEILVIFAAFSYSRALAVIGASVMVLTAGYILWTIRRVYLGAEYR
                     GPHAEALSPLTTREFLIAATL"
     gene            complement(1651..>3738)
                     /gene="nuoL"
                     /locus_tag="PKI05_06745"
     CDS             complement(1651..>3738)
                     /gene="nuoL"
                     /locus_tag="PKI05_06745"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOA51937.1"
                     /translation="PIRPLSGDWYTLAEFGSIKLALGYYIDALTLAMYVMVSFIATLI
                     HIYSFGYMHEELHEVNDPQVILGDGTTLRRPGRFARFFQYLSLFGFSMLGLVIANNLL
                     MTFVFWELVGICSYFLIGFYVERSSATSAGNKAFIVNRVGDFGMVLGLAALLTSCGTL
                     HFDHYQTTNGAHYAGLFFYAKAIQHPASELEPAEGSRSEDSQSNPETPPVTLSGPKGL
                     TYGLLVLAGLALLCGCLGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVYLVGR
                     IYPLLTPEVLIVVAVIGTITLFMAATIALTATDIKKVLAYSTISQLGYMMLGLGTGGW
                     VAGLFHLFTHAFFKALLFLGSGSVIHACGTNEMPQMGGLLRKMPWTAITMLIGCLAIA
                     GAGIPLWAGFSGYHSKDAILAQVYLFHTTNRFLGWIYYLALVSAALTAFYMFRLWFLT
                     FIGQPRNEHIFGHAHESPKSMTVPLVVLAFFAVVAGWSVPGTHWSLSALLEQARVTPL
                     LENSQVPLFGNVVIPHEHESHEAQIHQLVSLEAFTAGILGFAIAVVFYGARWLDPAKV
                     CQRFFGLYRFLLNKWYFDELYSRLFVRPVTWVASVAAWIDRAVIDWTVDHAAVAVRSL
                     AHLDDWLDRILVDGAVNRLAAAIYGMGEWFRQWQTGRIRNYILWIVVGLVSLFILGSI
                     YFGLGMHLVVGAS"
BASE COUNT         1067 a         1009 c          974 g          688 t
ORIGIN      
        1 caaagtggcg gcaatgagaa attccctggt cgtaaggggg ctgagagcct cggcgtgagg
       61 tccacgatat tccgcaccca gataaaccct ccggattgtc caaaggatgt agcccgctgt
      121 caaaaccata acggaggccc caatcacggc caatgctcgg ctgtagctga acgcggcaaa
      181 gataacgagg atttcaccga caaagccgca aagaccgggc aagccaagag ccgcaaagaa
      241 aatcacaatg gaaagggccg tgtaaacggg catccgaccg aagatgcctc cgaattgatt
      301 taggtcgcgg tgatgcaccc ggtcgtaaag aacgcccacc acaaagaaca tgccggccga
      361 cgaaattccg tgggcgatca tctggaacat ggccccgttc attcccatct tccaatactg
      421 tggatcataa aggcccggca tcaggctcca agcccctaag ccaaggacta cataacccat
      481 atggctgaca gagctatagg caaccagccg tttgaaatcc tgctgggcca tagctgccaa
      541 ggccccataa accatgctca cgattcccag aagacacacg aaccaggcta gctcgtagcc
      601 accttgagga cagatggggt agcagatacg gataatccca tacccaccca ttttcagcag
      661 aattcctgcc agaatcatgg agatcggcgt gggggcctcc acgtgggcat caggaagcca
      721 tgtgtgaact ggcacggcgg ggaccttgat caagaaacca atgagaagca gcaagaaggc
      781 ccaccaggct accgatttgc cccctaaaac aggctcctga aagggcgaat caggcatttg
      841 ccccagtttg gccaaggcaa tcaagttgaa cgtgtggagg ggtttttcgc ttggtgcgag
      901 ggacttgacc atgacttcct ggctaaggaa actttctcgg cgggcatttt cgggaagctg
      961 acgcacatcg cttgcaaagt agagcaaaag gatcgcaatc aacatcaaca cgctgcccac
     1021 cagggtgaaa aggaagaact tgatggccgc atattccctt cgcggtcccc cccacacacc
     1081 gattaaaaag tacatgggaa gaagcatgac ctcccaaaag acgtaaaaca gaaagaaatc
     1141 cagtgccaga aaaacaccca gcatccccgt ttctaaaaga agaaagagga tgctgtaagc
     1201 cttcacgtgt ttggtgacag accagctagc cgccgctgcc aggacagaaa ggaaagatgt
     1261 cagcaggaca agtggcaaac tgattccatc aactcctagg aaatagtcta tatggaaaga
     1321 actgatccag gggaccgcat aagcgtgttg ataacctggt tcgccggccg caaaacggac
     1381 atcgtgaagg acctgttcta atggattctg agacgccgcg tgtaggcccg gcaaaaccaa
     1441 tcgtaatgtc aacaagaaca ccaccagggt tacgccaagg gttgttcttc gaatggcacg
     1501 ctcggcgtgc gaggggaaga acatcaacac caaggcgccg aatgcgggca aaaacaccac
     1561 aatacttaag aaaaccaaac cagccaccat tgttgacaac ccgttatgtg cctttgatga
     1621 cctatttcca ccaagtgatg ccgtaaagga tcaggaagcc cccacgacaa ggtgcattcc
     1681 caacccaaag taaattgatc ccaaaataaa taggcttacc aagccaacaa cgatccaaag
     1741 gatatagttc cgaatgcggc ctgtttgcca ttggcgaaac cattcgccca ttccgtagat
     1801 ggccgcggca agccgattaa ccgcgccatc gacgagaata cggtccagcc agtcgtcaag
     1861 atgtgctagc gaacgcacag ccacggccgc atgatcgacc gtccagtcga taacggctcg
     1921 gtcaatccag gcagcaaccg atgccaccca tgtcacggga cgcacaaaca gccggctata
     1981 aagttcgtca aagtaccact tgttcagaag gaaacgatac agcccgaaga atcgctgaca
     2041 gaccttggcc ggatcaagcc agcgggcgcc gtaaaagacc actgcaattg caaaacctaa
     2101 gatacctgca gtaaaggcct ccaaggagac cagctgatgg atttgggctt cgtgcgattc
     2161 atgttcgtgc gggatgacga cgtttccaaa aagtgggacc tgtgagtttt ccaaaagcgg
     2221 tgtgacccgg gcttgttcta gcagagccga taggctccag tgtgtccctg gaacactcca
     2281 gcctgctacc accgcaaaga acgccaggac gacaagagga acagtcatac tttttggcga
     2341 ttcgtgggca tgtccaaaga tatgttcatt acggggttga ccaataaatg tcaaaaacca
     2401 tagccgaaac atgtaaaaag cggtcaaagc ggcagaaacc agggccaggt agtagatcca
     2461 accgaggaac cggttcgttg tgtgaaatag gtaaacctgg gcgaggatgg catccttgga
     2521 atggtaaccg ctgaatccgg cccaaagcgg gataccagct ccggcgatcg ccaagcatcc
     2581 aatgagcatc gtaatcgccg tccacggcat cttgcggaga agcccgccca tttgcggcat
     2641 ctcatttgtg ccgcaggcat ggattaccga tcctgatccg agaaagagca gggccttgaa
     2701 gaatgcatgg gtgaacaagt gaaataaccc cgcgacccat ccacctgttc caagacccag
     2761 catcatgtaa ccaagctgac taatggtcga ataggctagg accttcttaa tatcggtcgc
     2821 cgtcagcgca atggtggccg ccatgaataa tgtaatagtc ccgatgacag caacgacgat
     2881 caggacctct ggagtcaata agggatagat tctccccaca agatagacgc ctgccgcaac
     2941 cattgtggcc gcatgaataa gggcgctaac gggtgtcggg ccttccatag cgtcgggaag
     3001 ccagacatgt aggggaaact gggcgctttt gcctagacat ccgcacaaaa gggccaatcc
     3061 ggccaagacc aaaagcccat acgttagtcc ctttgggccg cttaacgtga ctgggggtgt
     3121 ctctgggttc gattgcgaat cctcgcttct tgacccctcc gccggttcta gctcagatgc
     3181 cggatgctgg atcgccttag cgtaaaaaaa aagtccagcg tagtgagctc catttgttgt
     3241 ttggtagtga tcgaaatgga gtgtgccgca gctcgtcaac aaagcggcta agcccaaaac
     3301 catcccaaag tcgccgacgc gattgacgat aaaggccttg ttcccggccg aggttgcgct
     3361 ggatcgctcg acataaaagc cgatcaaaaa gtatgaacaa atgccgacca gttcccagaa
     3421 gacaaaggtc atcaagagat tattggcgat caccagcccc agcatgctga acccgaatag
     3481 cgagaggtac tgaaagaacc gcgcgaatcg gccgggacgc cgtaacgtcg tgccgtcgcc
     3541 taaaatgacc tggggatcgt tgacctcgtg gagttcctcg tgcatgtacc caaaggagta
     3601 gatgtgaatc aaggtagcga taaagctcac catgacatac atcgcaaggg tcagggcatc
     3661 gatgtaatag cccagggcta acttgatgct cccaaattcc gccagcgtgt accaatcgcc
     3721 actgagtggc cgaatggg
//
LOCUS       DAOPTR010000294         3735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19739_length_3735_cov_3.798370, whole genome shotgun sequence.
ACCESSION   DAOPTR010000294 DAOPTR010000000
VERSION     DAOPTR010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19739_length_3735_cov_3.798370"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2479)
                     /locus_tag="PKI05_06750"
     CDS             complement(<1..2479)
                     /locus_tag="PKI05_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51938.1"
                     /translation="MVSIVVALAVWHSVGGVTRSPAQEPPLRNLLRLPPPDDEGGQTS
                     SPGASSETPGPLAKPTTEAPTSETVAESIPRITSEEITLRIQRVQADGELPDAVKSQV
                     VAAYERAIQELAAAEKFRNQAEQFRQQREAIQEEVKRLEAELAEVSATPFLPPSEGMT
                     LEEIQAAVAEAEKRLKAARLQRDAIDEEVTKRNAARQTATQRAEAAQKRLEAAREALA
                     ASPDPNQPPALTEAQRILRQTELEAARWELEAIKEELAYYDRADETLLPLRRRVAAQR
                     FRTVETVWNQWQQILAQRRQATAVSRVEQARARIAQLPEPLRALAEEVEKKAGEVADL
                     TTRVNQTHVQLTTVKQQLEELQQQFKATKERMEMVGMTGALGLWLRAQRNKLPETHTL
                     WQRIRKRQDDIRATQWTLLELSDAHSQMPPLEEELTRWRNRLLPLISEDHPEWETNLR
                     EVLAEKYTLLDEEVQLYSRWLNDLVDLDNTERAIVRTVEEYRRYIDEHILWIPNTRPV
                     RLSQLSDDLRLTLGVLRRLVSPTTLWDAGHALQIDLGTWPALWVLAGLVLAVWRLALF
                     QERRHLERLAKKVSQGAYHGFWPTLQVLALTVLGTGWWPAVLAFLAWRLGSPINATTM
                     TVAMSHSFWSTAEFLIVFGTLRRLCLPLGLGEAHFRWPSELTLFVRRQLRWFIPAGMV
                     LVYFCTVIHAMGDQRWEAGPGRIVFLLMLLLYAVLGHIMLRPKGSLLQAVRAHHPQSW
                     LYRGRTLWRLLAVGVPVGLMALTLAGYSYTAVQLMQRFRGSLMALLLVGVGEELFNRW
                     LIVRKQRIGWERFRQRMASEPQIVGEAK"
     gene            complement(3292..>3735)
                     /locus_tag="PKI05_06755"
     CDS             complement(3292..>3735)
                     /locus_tag="PKI05_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOA51939.1"
                     /translation="ALNAAIEAARAGEHGMGFAVVADEVRKLAERSNQAAGEITKLIK
                     ESTARVEEGAELSQKTEEALRRIVESVEATSQKISEIASAAVQQATSAEEVSKAMQNV
                     AQVVEQSAAGSEELASSSEELSAQAAALRELVGRFKVRAASVSVG"
BASE COUNT          728 a         1190 c         1045 g          772 t
ORIGIN      
        1 ttttggcctc accgacgatt tggggctcac tggccatgcg ctgccggaac cgttcccagc
       61 cgatccgttg cttgcggacg atcaaccagc ggttgaacag ctcttcgccc actcctacca
      121 agaggagggc catcaaactg ccgcgaaaac gctgcatgag ttgcaccgcc gtatagctgt
      181 aaccggcaag tgtgagggcc atcaggccaa cgggcacgcc caccgcgagc aaccgccaaa
      241 gtgttcgccc ccgataaagc cagctctggg gatgatgggc acgcacggcc tgaaggagcg
      301 atcctttggg ccgaagcatt atgtggccca gaaccgcgta aaggaggagc atcagcaaga
      361 acacaatccg ccccggaccc gcctcccacc gctggtcacc catggcgtgg atcaccgtgc
      421 aaaaatagac caacaccatg cccgccggta tgaaccaccg caattgacgg cgaacaaaca
      481 gggtgagctc gctcggccac cgaaagtggg cctcgcccaa tcccaaggga agacataacc
      541 gccgaagcgt tccaaagact atcaagaatt cggccgtgct ccaaaaactg tgtgacatgg
      601 ccaccgtcat cgtggtggcg ttgataggcg agcccaatcg ccaggccaag aaggccaaaa
      661 cggcgggcca ccatccggtc cccaagactg ttagggcaag gacctggagt gttggccaaa
      721 aaccgtgata ggccccctga gagaccttct tggcgagcct ttccagatga cgcctttctt
      781 gaaagagtgc caaccgccat acggccaaga ccaaccctgc caaaacccac aaggccggcc
      841 atgtccctaa gtcgatttgc agggcatgcc ccgcatccca aagcgttgtg ggcgagacca
      901 aacgacgaag gacccccaat gtcagacgca gatcgtctga aagctgagag agacgcacag
      961 gccgcgtgtt gggaatccag aggatgtgct catcaatata gcgtcgatac tcctcgacgg
     1021 tcctgacaat cgcgcgctcc gtgttatcca aatccaccag atcgttgagc caccggctgt
     1081 agagttgaac ttcttcatcc agcaatgtgt atttctcggc gagaacctct cgcaagtttg
     1141 tttcccactc gggatggtcc tccgaaatga gcgggagaag ccgattccgc caccgagtta
     1201 attcctcctc caaaggaggc atttggctgt gagcgtcact caattccaag agcgtccact
     1261 gtgtcgcccg aatgtcatcc tgtcgtttgc ggattcgttg ccagagggta tgggtttccg
     1321 gaagtttgtt cctctgtgcc ctcagccaca gccccaaggc ccccgtcatg ccgaccatct
     1381 ccatacgctc tttggtggcc ttgaactgtt gctgaagctc ttccaactgt tgtttgaccg
     1441 tggtgagctg aacgtgcgtt tgattgaccc gcgttgtcag gtcggcaact tctccggcct
     1501 ttttctccac ctcctcggcc agtgcccgca gcggttccgg aagttgggca atccgtgccc
     1561 gggcctgctc cacccgggag accgccgtgg cctgtcgccg ttgggcaagg atttgttgcc
     1621 actgattcca aaccgtttcc accgtacgga agcgctgggc cgccacccgt ctccgcagcg
     1681 gcaagagggt ttcgtcggct cgatcataat aggccagttc ctccttaatg gcctccagct
     1741 cccaccgggc cgcttcgagt tcagtttgac gcagaatccg ttgggcctcc gtcaaagccg
     1801 gtggctggtt cgggtcggga gaggcagcca aggcctcgcg ggctgcttct aaccgttttt
     1861 gggcggcctc cgcacgctga gtcgccgttt gccgggcggc atttcttttg gtcacttcct
     1921 catctatggc gtcgcgttgc agccgggccg ctttaagccg cttttccgcc tccgccactg
     1981 ccgcctgaat ttcctctaac gtcatcccct cggatggtgg gaggaacggg gtggcactca
     2041 cctcggccag ctctgcctcg agtcttttga cctcctcttg gatggcctcc cgctgctggc
     2101 ggaactgctc ggcctgattt cgaaacttct cggctgccgc caattcttgg atggcccgct
     2161 cgtaggcggc caccacctgt gacttaaccg cgtcgggcaa ctcaccgtct gcctggaccc
     2221 gctggatgcg cagcgtgatc tcctcgctcg tgatgcgggg gatactttcc gccacggttt
     2281 ccgacgtcgg cgcctccgtg gtgggcttgg ccagtggccc aggagtttcc gatgatgccc
     2341 cgggagacga tgtctgccca ccttcgtcgt ctggaggagg cagccgcagg agattcctca
     2401 agggtggttc ctgagcggga cttctcgtca caccccccac ggaatgccat acggccagcg
     2461 ccacgacaat actcaccacc cacagtgggc agctcgttct taggacgctc caaatggttc
     2521 ctagcgcacg gttcattggt tatggctgcg gatgatcggc cagcttgctg gacgtgtctg
     2581 gttcgttgcc aagccagcac gccaccaaag ttcttccttt tcacgtggta aaaaacggat
     2641 tcctgttata gactttcccg ttggaaacag gggaaacacc aggcccaaat ggcattgtgg
     2701 ctgtctcggg ccgaggatgc aatgtcaatt taggaatccc cgaggcctac ctgtggaaag
     2761 agtaactgac atccgcgcct gatggtctgt cgatgatcgt cctaactcgc cgaaggttca
     2821 aggctacctg cacccgtctg tggcaggaga agatgctaat gtgactgtgc tcacggatga
     2881 accagcttga aggtatagct ggcgagtcag tccaagaaac gaaaagcggg gcgtccttgc
     2941 cccggaccat tgcaacgtgg aggcgatcac ttcaagctca cgcggacgac ttacagtcta
     3001 tcccaaaaag tccccctctc cctccgggag agggttgggg tgagggtaag ccatcgtcgc
     3061 agtccatcga gaaaaccagc agcgattcga gccaccctca ccccccgccc ctttcccgcc
     3121 gagcgggaga ggggaggcgg tggggcggac tccaaaatgg gccgccacac ctcggcccga
     3181 accacttttt ggcaaaataa agaaccacgg gcacggtggg gcgcgtgccc gtggtctcgc
     3241 gggagaaggt gacgatgaga gactccgtta ctttacatcc gttgccctct ttcaaccgac
     3301 agaaaccgat gctgcccgca ccttgaagcg tccaactaat tcgcggaggg ctgccgcctg
     3361 ggcggacagc tcttcgctgc tggaagccaa ctcctcgctg ccagccgccg actgctcgac
     3421 gacctgggca acgttttgca tggccttgct aacctcttcc gcactggtgg cctgttgcac
     3481 cgctgcgctg gcaatttcgc tgatcttctg acttgtcgct tccacgctct ccacgatccg
     3541 ccgcagggcc tcctccgttt tctggctcag ttcggcacct tcctccaccc gggcagtgga
     3601 ctccttgatg agcttggtga tttcgccggc tgcctggttg gacctctcgg ccaacttgcg
     3661 gacctcatcg gccaccacgg cgaagcccat accgtgctca cccgcccgag ctgcctcgat
     3721 cgccgcgttg agggc
//
LOCUS       DAOPTR010000295         3728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19787_length_3728_cov_3.196025, whole genome shotgun sequence.
ACCESSION   DAOPTR010000295 DAOPTR010000000
VERSION     DAOPTR010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19787_length_3728_cov_3.196025"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /locus_tag="PKI05_06760"
     CDS             complement(<1..1228)
                     /locus_tag="PKI05_06760"
                     /inference="COORDINATES: protein motif:HMM:NF019219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HOA51940.1"
                     /translation="MKTFQPLVLRILSVTLLLLVPALLARGEEAIEPFDSGQPFTVNN
                     RIDEILAKKWKSLGIQPAQICSDGVFVRRVYLDVIGTLPTAEEAQRFIDDTSRNKRQR
                     LIEELLDRPEYADYWTMRWGDILRIKAEFPINLWPNAVQAYNRWLWSALATNKPYDQF
                     AMELLTSSGSNFRVGAVNFYRATQDRSPKGIAQAVALTFMGERAEKWPPERLEGMAAF
                     FSQISFKPTLEWKEEIVTWDPFHATESGDPPPQEAVFPDGVKIKLPADRDPRKVFAEW
                     LVSPKNPWFAKNIVNRIWYWLFGRGIIHEPDDIRTDNPPVVPELLSYLESELVQSKWD
                     LKHIYRLILNSNTYQLSCVPRSQHPERDTYFACYPLRRLEAEVLIDAICQITGTSEDY
                     WSMVPEPYTVVPQGERA"
     gene            complement(1505..3133)
                     /locus_tag="PKI05_06765"
     CDS             complement(1505..3133)
                     /locus_tag="PKI05_06765"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008700565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOA51941.1"
                     /translation="METLVALCKRRGFLFQSSEIYGGLNGFWDYGPLGVELKRNIREA
                     WWWDNVLSHNELITPLGAPSPYQMVGLDSTIIMHPQVWKCSGHYDLFHDYMVDCRETK
                     KRYRYDQVQGRWVSYKGQRIFVATEAVGDEALAETEHKALKYFGLRPKDSEELVWESP
                     IVSLTTVEDLSLVLAPDAKTLGTLTPPREFNLMFKTYVGALSGEENAAFLRPETAQGI
                     FVNFKNVLDSTRVKIPFGIAQIGKSFRNEITPRYFTFRSREFEQMEIEFFCRPETSRA
                     WYEYWRKRRYQWYLDLGLAGERLRLRDHSPEELSHYSCGTADVEYAFPFLAPGDFGEL
                     EGIAHRGDFDLRSHMEGKLIRKGNELVVELDANGQPKYRGSGRDLTYFDEETRERFIP
                     HVIEPSAGADRAALAFLCEAYAEDTVPDEKGNPTKRVVLRLHPRLAPTKVAVFPLVKK
                     DGMPEIAQSIYAALKPHVNCFYDEKGAVGRRYRRQDEVGTPFCITVDSQTLADNTVTV
                     RDRDSLRQWRIKVDDCVEVIKNLVSGKERPPELA"
BASE COUNT          853 a         1049 c          990 g          836 t
ORIGIN      
        1 cggccctttc cccttgggga accacggtat agggttccgg caccatgctc cagtagtcct
       61 cactggtgcc ggtgatttga caaatggcgt cgatgagcac ctcggcttcc agccgccgca
      121 aggggtagca ggcaaaatag gtgtctctct cgggatgttg gctgcgagga acgcacgaca
      181 gttggtaggt gttggaattg aggatcaaac gataaatatg cttgaggtcc catttcgact
      241 ggaccaattc cgattctagg taggacaaca attcgggaac aacaggagga ttatccgttc
      301 gaatgtcatc tggctcgtgg ataatacccc tgccaaaaag ccaataccaa atacggttga
      361 cgatgttttt tgcaaaccac gggtttttgg gcgagacgag ccactcagcg aaaaccttcc
      421 gtgggtctcg gtcagccggt agcttgatct tcacaccgtc ggggaaaacg gcctcctggg
      481 gtggtgggtc accgctttct gtggcatgga agggatccca cgtgacaatt tcttctttcc
      541 attccagcgt gggcttgaaa ctgatttggg aaaagaaggc cgccatccct tcaagccgct
      601 ccggcggcca cttttccgcg cgctctccca taaacgtcaa agccacggcc tgggctatgc
      661 ccttgggaga acgatcctgt gtcgcccgat aaaagttcac tgcccctacc cgaaaattgc
      721 tcccgctgga ggtgagcaac tccatggcga actggtcgta aggcttattt gttgccaagg
      781 ccgaccacaa ccagcgattg taggcctgaa cggcattggg ccagaggtta atgggaaatt
      841 ccgccttgat ccggagaata tccccccacc tcatggtcca gtagtctgcg tactccggac
      901 gatcgagcaa ctcttcgatc agacgctgcc gtttgttgcg cgaagtgtcg tcaatgaatc
      961 gctgggcctc ctcggccgtg ggcaaggttc caataacatc cagatacact cggcgaacaa
     1021 aaaccccgtc ggaacagatc tgcgccggct ggatgcccaa agacttccac tttttcgcca
     1081 aaatctcgtc aatgcgatta ttgaccgtaa aaggttggcc actatcaaaa ggctcaatcg
     1141 cctcctctcc ccgagccaaa agcgccggca caaggagaag gagcgttacg ctcaatatgc
     1201 gcaagaccaa tggttggaat gttttcatga gattcacaaa acgttgattg caaaaaacaa
     1261 ggctggcaca tcaccgacga gaggcgacct tcacctcaaa agaataatgt cacgcggggc
     1321 caaaaaggca cgctttcttg tcagaaatcc ccgatggtcc ccgcgccggt cgggggtctc
     1381 ttgaatttga cgctgatcag gcaacgaaga gcccgacatg ctggcctagc cggagcctgt
     1441 ccaagaggcc ataagaaaac tctggacctc tcgtcgtttc cccgagcccc tgtcgtgcta
     1501 gagattacgc cagctcgggt ggccgttctt tgccggagac gagattctta atgacctcga
     1561 cgcagtcatc gactttaatt cgccactgtc ggagggaatc gcgatcccgc actgttacgg
     1621 tattgtctgc caatgtctgg ctgtcaacag tgatgcaaaa tggcgtaccg acctcatctt
     1681 gcctgcgata ccgcctcccc acagccccct tctcgtcata gaagcaattc acgtggggct
     1741 taagggcagc atagatgctt tgggcaattt cgggcatgcc atcttttttc acgagtggaa
     1801 agaccgccac tttggtcggt gccaaacgag gatggagcct taacaccacc ctttttgtgg
     1861 ggttcccctt ctcgtcgggc accgtatcct cagcatacgc ctcacagagg aacgcaagcg
     1921 ccgcgcggtc tgccccggcc gacggttcaa tgacatgggg aataaatcgc tccctagtct
     1981 cttcgtcaaa ataggtaaga tctcgaccac tcccccgata cttgggctga ccattggcgt
     2041 caagttcgac taccagctcg tttcccttgc gaatcagttt gccttccatg tggctgcgga
     2101 ggtcgaaatc gccacgatgg gcaattcctt ccaattcgcc gaaatcacct ggggccagaa
     2161 aaggaaacgc atattcaaca tcggcggtgc cgcacgaata gtggctcaat tcttccgggc
     2221 tgtggtcccg caggcggagg cgctccccgg ccaaccccag gtcgagatac cattgatacc
     2281 gccgcttccg ccaatactcg taccacgctc gagaggtctc cggtcggcag aagaactcga
     2341 tctccatttg ctcaaattct cgggagcgaa aggtaaagta tcgaggcgta atttcattgc
     2401 ggaaactttt cccaatttgg gcaattccaa aggggatctt tactctcgta ctgtcaagca
     2461 cgttcttgaa attaacaaaa atcccttggg cggtctctgg gcggaggaat gccgcatttt
     2521 cttcaccgct aagcgcgccc acatacgtct taaacatgag attgaattcc cgcggcggag
     2581 tcagcgtgcc caatgtcttc gcatcagggg ccagcacaag cgagaggtcc tccaccgttg
     2641 tcagggacac gatgggtgat tcccagacaa gctcctcgga gtccttggga cgcaacccaa
     2701 agtatttcag ggccttatgt tcggtctcgg cgagggcctc atcgcccacg gcctccgtgg
     2761 cgacaaagat ccgttgcccc ttgtacgaaa cccagcgtcc ctgaacctga tcgtaacggt
     2821 accgtttctt ggtctcccga cagtcaacca tgtagtcgtg gaagaggtca taatgaccag
     2881 aacacttcca cacttgagga tgcatgataa tcgtcgagtc gagccccacc atttgatagg
     2941 gagatggcgc ccccagcgga gtgattaact cattgtgcga gaggacgttg tcccaccacc
     3001 aggcctcacg gatattccgt ttgagttcca ctcccaaagg gccgtaatcc caaaatccgt
     3061 tgaggccacc ataaatctcg ctcgattgga agagaaagcc tcgcctcttg caaagggcaa
     3121 caagggtctc catctgcatg actaaacggc tcctgggata tacctctcgt gagacttcgg
     3181 ttgcttggtt ggcgttaacg tcttccgaga atttcttggt cggttacaac gaccaatcga
     3241 ttgcgggagc ggctcaaccc caatcacgcg attctaggct tcgcgcatgg tagcaagttg
     3301 tgctaagatg gcgaaacgtc tagtttactc gctagtgtcc gaaccgtcca cgagacccat
     3361 cctctggagg ccggtttggg ggggtcactg cggccccgtt gccgtgcagg gaaagacgtg
     3421 cgaaatatgt tttgagggtt ccgcatgata cggagttacg ggggcatgcg ccattagaaa
     3481 gcgagagccc cgtaaaaagg gtgtaaccga gctgatcatc ttttctcgag ggttcctttt
     3541 ttggtgatta tcgcggccct ggatttggta caatggacaa ggagcctatc ccaaatttcc
     3601 ctctccctct gggagagggt tggggtgagg gcaagcaatc gtcggaatcg atcgagaaga
     3661 ccaatagcga tttgagccac cctcaccccc ggcccctctc ccgccgaacg ggcgagggga
     3721 ggttgtgg
//
LOCUS       DAOPTR010000296         3725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19804_length_3725_cov_4.205995, whole genome shotgun sequence.
ACCESSION   DAOPTR010000296 DAOPTR010000000
VERSION     DAOPTR010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19804_length_3725_cov_4.205995"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="PKI05_06770"
     CDS             <1..820
                     /locus_tag="PKI05_06770"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HOA51942.1"
                     /translation="TWRGRGRVVVGQVNGRAVRVGDFPIAIDYRSFARRANAKEVLAV
                     RDMIRAKLPDRKIILGVDRLDYTKGIPHRLEAYREALRRHPDLHGRTTLVQVVVPSRT
                     GVPQYRRLRARIERLVGTINGEFTRPGWVPIHYIFRSLSPEELLGYYRAADIALITPL
                     KDGMNLIAKEYCTTNVDNDGVLILSEFAGAASQLQNGAILVNPFHITEVADAIYFACT
                     MPISERKRRMKKLRQVIRKQDIFWWVDSFLRSAFSKHLSEFPQLEEYMPLTSTS"
     gene            complement(941..2167)
                     /locus_tag="PKI05_06775"
     CDS             complement(941..2167)
                     /locus_tag="PKI05_06775"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOA51943.1"
                     /translation="MVQIVRHVALCSVLLFAANGKAFGQNTAQPVVSDEQIPDGSLDP
                     FLGKPDLQKQILFSGERFPNIVVTKKGTVLAVWGSQSVRCRRSEDGGQTWEKEIAIAV
                     PGFHGGGVTVDETNGHIFVFVQAQHPPAPLTVYRSRDDGKTWEQTPVTIHPDQKGHTP
                     SMHMAEHGITLCYGPAKGRLIRPARVYDRPRGYNTAIYSDDGGATWHTSAPFPDEGTG
                     EGAIAELTDGRLYYSSRKHHFENIADFSWKRFFAWSYDGGETWTHLAVSDVLPDGPRY
                     RGEERRGSNYNGHFGMMAGLTRLPVRGRDILIYSNSDHEGHERIRLTVWAGFDGGQSW
                     PIKRLVHEGMSAYSSLAAGRPGTPSEGWIYLFFEHGDGKQQYAGRTVARFNLAWLLAG
                     EKTADGDIPTWLAERR"
     gene            complement(2349..2513)
                     /locus_tag="PKI05_06780"
     CDS             complement(2349..2513)
                     /locus_tag="PKI05_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51944.1"
                     /translation="MTIHAGRVILLKDARNGRVTIPSHIGRVAVPGDQISGRRGRRPS
                     LLFTDATRSL"
     gene            complement(2960..>3725)
                     /locus_tag="PKI05_06785"
     CDS             complement(2960..>3725)
                     /locus_tag="PKI05_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51945.1"
                     /translation="YADGYANVNYNAYLDMQFQFIATHPALFGLGGMHIWRSGYCDEE
                     RLRWCARLFRHYALEGNTNRLSADPYMLTHIENPDFVEGLKHWEVSPARENSVFATAQ
                     KGFGKFIGRYYRGPDSFLVTRRDPEKPNVVRQVIRNLEPGRLYSVKMFTGDYGAFQSG
                     NSTRELHAVSLQIKGAELLSGPQYRYQQAFPTRERLGNFTEQKPFWLNLHWLVFRATA
                     PTAELVISDWKTPQNPGGPQDQTLLYTYVEVKPYLD"
BASE COUNT          839 a         1030 c         1027 g          829 t
ORIGIN      
        1 cacatggcgc ggtcgggggc gtgttgtcgt tgggcaggtg aacggtcgcg ccgtgcgggt
       61 tggcgatttt cccatcgcca ttgactatcg atcgtttgca cggagagcca acgcgaaaga
      121 ggttctagct gtgcgggaca tgatccgcgc caagttgccc gaccgaaaaa tcattttggg
      181 tgtcgatcgt ctggattaca cgaaaggaat tccccatcgt ctggaggcgt atcgggaggc
      241 gctgcggaga catcccgacc ttcatggcag aacgactctt gttcaagtgg tggttcccag
      301 ccgaacggga gtcccccagt atcgacggct gcgcgcccgc attgaacgat tagtcggcac
      361 cattaacggg gaatttaccc gtcctggatg ggttccgatc cactacatct ttcgctcgct
      421 gagtcccgag gagcttttag gatattaccg agcagccgac atcgcgttga tcacgcctct
      481 taaggatgga atgaacttga ttgcaaaaga gtactgcaca actaacgtcg ataacgatgg
      541 cgttttgatt ctcagcgaat tcgcaggtgc ggcatcgcaa ctacaaaatg gggcgattct
      601 cgtcaatcct tttcacatca ccgaagtggc tgatgcgatt tatttcgcgt gcacgatgcc
      661 catttcggaa cgcaagcgac ggatgaaaaa actccgtcaa gtcattcgta aacaagatat
      721 cttctggtgg gtcgattcat ttctccgcag tgcgttttcc aagcacctga gcgaatttcc
      781 ccagcttgaa gaatatatgc cgttgacctc aacatcatag gcagagggcc cgtggtagcg
      841 cccaggaatg ccacgctacc accgcgtcgc caacttgcaa aaaatcaaca gcgcggtgtg
      901 tgacgagtat gaaccggctc ttaggtcccg atatgttttg ctaccggcgt tcagccaacc
      961 atgtggggat gtccccgtca gctgtctttt cccctgccag cagccatgcc aaattgaacc
     1021 gggcgacggt tcgcccggcg tattgctgtt ttccatcacc gtgttcgaag aagagataaa
     1081 tccagccttc gctgggtgtg cccgggcgac cggcggcaag cgacgaatag gccgacattc
     1141 cctcgtggac aagacgcttg atcggccagg attggcctcc atcaaaaccc gcccaaaccg
     1201 taagccgaat ccgttcatgc ccctcgtggt cggagttgct gtagatcaat atgtcacgcc
     1261 ctcggactgg taaccgagtt agacctgcca tcataccaaa gtgaccgttg taatttgagc
     1321 cgcgacgttc ttccccgcga tagcgcggac cgtcaggcag cacgtcgctg accgccaagt
     1381 gtgtccaagt ttctccaccg tcgtagctcc aggcgaaaaa acgcttccaa gagaaatcgg
     1441 caatattttc aaagtgatgt ttgcgtgagc tgtaatacag gcggccgtcg gtcagctcgg
     1501 cgatcgcacc ttcccccgtg ccctcatcgg gaaaaggggc acttgtgtgc caagtggcac
     1561 cgccgtcatc gctgtaaatg gcggtgttat agccccgggg tcgatcatag actcttgcgg
     1621 ggcggattag cctccctttg gccggaccat agcacagggt gatcccgtgc tcggccatgt
     1681 gcatggacgg cgtatggcct ttctggtccg gatgaatcgt cacgggagtt tgctcccacg
     1741 tttttccgtc atcacgactt cggtaaacgg ttaatggtgc tgggggatgc tgagcctgaa
     1801 caaaaacaaa gatgtgacca tttgtttcat cgacagttac accaccccca tgaaatcctg
     1861 ggactgcgat tgcaatttcc ttctcccagg tttgcccacc atcctcgctt cgacgacaac
     1921 gaacagactg acttccccat acggcgagaa ctgtcccttt tttggtgaca acaatattcg
     1981 gaaatcgctc tccgctgaac aaaatctgtt tttgaagatc cggctttccc aaaaaaggat
     2041 cgagagagcc atcggggatc tgctcgtccg acacaactgg ctgagccgta ttctgtccga
     2101 acgctttgcc attagcagcg aaaagcagga cactgcaaag cgcgacatgc cgaacaatct
     2161 gaaccataac agttctcata tgttcgacct caagtgaccc aaacgatgcg actctcccga
     2221 ccatagccca ccgacgcggc agtttacaat agacacgtgg aatctgtgcg caagcatagg
     2281 ccttcagcag tatcatcgta accgttcaca gcgcagatcg ccacttcttg ggacgcgaga
     2341 gggctgcatc ataaggacct ggtggcgtcg gtgaagagga gggagggacg gcgtcctcgc
     2401 cgtccgctga tttggtcgcc ggggacggcg actctcccga tgtggctcgg gatagtgacc
     2461 cggccattac gggcatcttt cagaaggatc acccgtcccg cgtgaatggt cacgtataat
     2521 gtcatccttc ttgctggcac aagaatccga cagagggggt tcagcggaag acgcggcagt
     2581 aatggcccgg aacaacgtgt agcggacttg gccacggtcg gttgtgacgt gatgcggaaa
     2641 atgacgcagc ggcaccgcac gtcaaggaat agggtggtca cggcgtgtga ccgaagctca
     2701 ctcgtcggac gggctcgggc ttaagagggc cgatctgccc cgcgactctg gtgcggctat
     2761 ctggagccta tcccaaagtt ccctctccct ctgggagagg gttggggtga gggcaagcga
     2821 tcgttggagt cgatcgagaa aacgccagcg atttgagcca ccctcacccc cgacccctct
     2881 cccgctgaac gagagagggg aggtcgtggg ataggttctt attacgcagg cagaggttcc
     2941 actcatttcg ctacccaccc tagtcgaggt agggcttcac ctcgacgtat gtgtacaaca
     3001 gcgtttggtc ttgcggtcca cctggattct gcggggtttt ccaatcggag atcaccagct
     3061 ccgcagtggg agccgtcgcc cgaaacacaa gccagtgcaa attaagccaa aacggctttt
     3121 gctcagtgaa gttgcccaag cgttcccgtg ttggaaaggc ctgttgataa cggtactgcg
     3181 gtccggaaag taattctgcg cctttgatct gaagggacac ggcgtgtaac tcgcgagtag
     3241 agttcccact ttggaaagcc ccgtagtccc ctgtgaacat ttttaccgag taaagccgtc
     3301 ctggttcgag gttccgaata acctgccgaa ccacgttggg cttttccggg tcgcgccgcg
     3361 tgacgagaaa gctgtccgga ccacggtagt atcgaccaat aaacttgcca aatccctttt
     3421 gagcagtagc aaaaacggaa ttttcgcggg cgggtgaaac ctcccaatgc ttcaaacctt
     3481 ccacgaagtc gggattctcg atatgtgtga gcatgtatgg atcagcgctc aggcgatttg
     3541 tgtttccttc caatgcgtag tgacggaaga gacgagcaca ccatcgcagt cgttcctcgt
     3601 cgcagtaccc acttcgccag atatgcatac cgcctagacc gaataaggcc ggatgggtgg
     3661 cgataaactg aaactgcata tccaggtagg cgttatagtt gacgttcgca tagccatcgg
     3721 catac
//
LOCUS       DAOPTR010000297         3721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19827_length_3721_cov_3.291326, whole genome shotgun sequence.
ACCESSION   DAOPTR010000297 DAOPTR010000000
VERSION     DAOPTR010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19827_length_3721_cov_3.291326"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PKI05_06790"
     CDS             complement(<1..293)
                     /locus_tag="PKI05_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51946.1"
                     /translation="MVENLLEVHWWHWCLFSVLIVVLLILDLVVFHRREHRPTLRESA
                     ILSAFWIGLALVFNTLVWWWGWQLHGNSEAGVKFLTGFVVEKSLSVDNLFVFAV"
     gene            complement(422..1498)
                     /locus_tag="PKI05_06795"
     CDS             complement(422..1498)
                     /locus_tag="PKI05_06795"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOA51947.1"
                     /translation="MATQAIEGIFTPNMVPLDERGRINEPELRRYLDWLIAKGVHGLY
                     PNGSTSEFVRFTPEERRRIIEITVDQVAGRVPILAGAAEANVQETIKACEYYARLGVR
                     AVAIVCPFYYRVSPDNIYSYFKEIAKHSPVDVTIYNIPWLASPLDVPTIVRIAEECPR
                     VVAIKDSSGDLPQMMRMITAIRARRPDFSFLTGWDAVLFPMLLLGCNGGTNATSGVVP
                     EITRAIFEAVKAGRIDEARRWQYRLLVYNDALFGAGDFPEGFRIGVEARGFRMGVGRQ
                     PQSEASRERLTRLRRQLEELLVEDRLESIPDGAMASSRATSEMTRSGVARVSGATEEN
                     VREQVERVVREVVHVLAERNVAVN"
     gene            1648..>3721
                     /locus_tag="PKI05_06800"
     CDS             1648..>3721
                     /locus_tag="PKI05_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51948.1"
                     /translation="MPLSAQMHLILPILGIIAYLTLFFAWPLLSISQVFQRPFRRYEL
                     FQLLLTPEELLKSWLGSHGEFSIFDRLPIFCAAAIILAAGIAWGVAISRVMGVKLSLL
                     EIVSIALPVGLNVISTLVLLGGLFGLLRQRWLLAAIVFLPPTLYIIPWQSLLRLVLKP
                     DVIVQRLRPIFPVQRAMTVQSLPEKILSWVCIGATLTAIVIILLAASLPPVDFDVREY
                     HLQCPKEFFQQGRITFLPHNVYGNMPLGLEMHALLGMLLLNDFWYGALAGKVVMATFA
                     LIGAAALLHWARSRGASSPWVAPAIFISVPWVISVSTAGLNDIGLAVYIFTAFCAASK
                     LVSILTDADQLYPALERGADWATITRENQQPERNPLNKGCPIRRESWAWVIIAGYLAG
                     AAAACKYTGLLFGGFLIGMFLLVTLAHRIPVRSLLLMLGLYGLGIILGAGGWYIKNLV
                     FSGNPVYPLAYEWLGGTSWNPEKAARWARIHSPHDFSVRMLLVDLGRVFFTSEWQSPL
                     ITPLAFLGLLRVSKQRLFEKATAVMAIVLFLLWWCLTHRIDRFWLPMLPFLCVLATVG
                     FDWTDRLWWRWFVTGICLIAMICNGLVITSGAAADNRYFAPLAQLRNDPGRIGADHAL
                     LNELFARDHAEGKKKKLLAVGEAAVFDLEMPVLYATCFDDQPWEILTKGKTPKEVRET
                     LHREDVGYL"
BASE COUNT          833 a         1090 c          926 g          872 t
ORIGIN      
        1 acggcaaaca caaaaagatt atcaaccgag agggacttct ctacgacgaa acctgtgaga
       61 aacttcacgc cggcctcaga gttgccatgg agctgccacc cccaccacca aacaagtgta
      121 ttgaacacca gtgcgagccc gatccaaaac gcggagagga tcgccgattc ccgcagagta
      181 ggcctgtgtt cccgccggtg gaaaacaacc agatcgagaa tcaacaagac gacaatcagg
      241 actgagaata agcaccaatg ccaccaatga acttccagca gattctcaac caagaggcac
      301 tcctttctcc gacccaacac tccaaatccg tagctttgga aatggccaac cactcggaga
      361 gtggggcgca ggccttcgcg ccctcggatc atggatcatc acaaaagacc tacatcatcc
      421 ttcagttgac ggcaacattc cgttccgcca aaacgtgaac aacctcccgc acaaccctct
      481 ccacttgttc ccgaacgttt tcctccgtgg cccctgaaac ccgggcaaca cctgatcgtg
      541 tcatctccga tgtggcgcgg gaactcgcca tcgcgccgtc cggaatcgat tccaaacggt
      601 cctccaccag gagctcctcc aattgtcgcc gcaatcgggt tagccgctcc cgactcgcct
      661 cagactgcgg ctgacgcccc acacccatgc gaaagccgcg ggcttccact ccgattcgga
      721 atccttctgg aaaatcaccc gccccaaaaa gggcatcgtt gtacacaagc aatcggtact
      781 gccaccgtct cgcctcgtcg attcgtcccg ccttcaccgc ctcgaaaatg gcccgggtaa
      841 tctccggtac caccccactc gtggcattcg ttccgccatt gcaacccaaa agtaacatag
      901 gaaacagaac agcatcccaa cctgtcaaaa aggagaaatc tggacgacgc gcccgaatgg
      961 ccgtgatcat tcgcatcatt tgcggaagat cacccgacga atccttaatc gccacaactc
     1021 gcggacattc ttcggcaatc ctaacgatcg tcggtacatc caggggcgag gccaaccatg
     1081 ggatgttgta aatcgtcaca tcgaccggac tatgtttggc aatctccttg aagtagctgt
     1141 aaatattgtc cggactgacg cgataataga acgggcagac aatagccacc gcccgtacac
     1201 caagcctcgc atagtattcg caagccttga tcgtttcttg cacattggct tcggcggcac
     1261 ccgccaagat ggggactcgc cccgccacct gatccaccgt gatctcaatg atccggcgac
     1321 gctcctcagg ggtaaaccga acaaactcac tggtggaacc gttgggataa agcccatgaa
     1381 cgcctttcgc aatcaaccaa tccaaataac gccgcaactc cggttcgttg atccgtcccc
     1441 gctcgtcgag gggaaccatg ttcggtgtaa aaatgccttc aattgcctgg gttgccatac
     1501 gacaaggacg ccttatgctg gaaacggagg gaaatcggta gccaactttg catgctccgg
     1561 atggtcaaaa tgaaactccg caatcatcgg tcgggttcaa aggacatcag gcaggggact
     1621 aagccctcag aattaccgac cgcaggcatg ccgttgtctg cgcaaatgca tctgattttg
     1681 cccatcctag gaatcatagc ctatttaacc ctttttttcg cctggcccct cctcagcatt
     1741 tcgcaggtgt ttcaaagacc tttccgacgg tacgagctgt tccaattgtt gctaacgccc
     1801 gaagaactcc ttaagtcatg gctggggagc cacggagaat tctctatctt cgacagactc
     1861 cccattttct gcgccgcagc cataattctc gccgccggta tagcttgggg agtcgctatt
     1921 agccgggtta tgggagtgaa gcttagcctt ctggaaatcg taagtatcgc cctaccggtt
     1981 ggacttaacg tcatttcaac gctcgtcctg ctcgggggcc tttttggttt actccggcag
     2041 cgatggcttc ttgcggcaat cgtgtttctt cccccgacgc tctacattat cccgtggcag
     2101 tcgttgctga ggctggttct caaacccgac gtaattgtgc aacgcctccg ccctattttt
     2161 cccgttcaac gcgccatgac tgtacagtcc ctgccagaaa aaattctttc gtgggtctgt
     2221 atcggggcaa ccttaaccgc aattgtgatc atccttttag ctgcgtcgct cccgcctgtg
     2281 gattttgatg ttcgcgaata ccatctccaa tgtccgaaag agtttttcca gcagggtcgt
     2341 atcacttttt tgccgcacaa cgtttacgga aacatgccac tgggtctgga aatgcacgcc
     2401 ctgctcggga tgctccttct taacgatttt tggtacgggg ccttggcggg caaggttgtc
     2461 atggcaacat tcgcgttaat tggggcggca gccttactcc actgggcgcg atcaagaggt
     2521 gcgagttccc cttgggtagc accagccatt tttatctcag tcccctgggt catctcggtc
     2581 tccacggccg ggctgaacga cattgggctc gccgtttaca tattcacggc tttttgtgcc
     2641 gcttcaaagc tcgtctcgat cctgaccgac gcggatcaac tctatccggc actcgaacga
     2701 ggggctgatt gggccactat cacgagggag aatcaacagc ctgaaaggaa cccattgaac
     2761 aagggatgtc ccatccgccg ggaaagctgg gcctgggtga tcattgccgg atacctggct
     2821 ggcgcggccg ccgcgtgcaa atacacgggc ttgctattcg gaggcttttt gatcgggatg
     2881 tttctgttgg taacgttggc ccatcgcatc cccgtacggt cgctcttgct gatgcttggc
     2941 ctctacggat tgggaataat cctcggtgca ggcggctggt acattaagaa cctcgttttc
     3001 agtggtaacc cggtttaccc ccttgcttac gagtggctgg gcggcacgtc atggaatccc
     3061 gaaaaggccg cacgctgggc acgaatccac agcccccacg atttttccgt tcggatgtta
     3121 ctggtcgatc tcggccgggt ttttttcacc agcgaatggc aaagtcccct tatcacacca
     3181 ctggcattct tgggtctctt gagggtcagt aaacaaagac ttttcgaaaa agccacagcg
     3241 gtcatggcga tagtcctttt tttactttgg tggtgcttga cccaccgaat tgaccggttt
     3301 tggcttccta tgttgccctt tttgtgtgtt ttggcaactg tcggatttga ctggacggac
     3361 cgactatggt ggcgttggtt tgttaccggg atttgtctta tcgccatgat ttgcaatggg
     3421 ttggtaataa cgagcggagc tgcggccgat aatcgctatt ttgccccgct tgcgcagttg
     3481 cgcaacgacc caggtcgtat tggtgcggat catgcccttc tgaacgagct tttcgcaaga
     3541 gatcacgctg agggcaagaa gaagaaactt ttggccgttg gcgaggccgc cgttttcgat
     3601 ttggaaatgc cagtccttta cgccacctgc ttcgacgatc aaccgtggga gattctgacc
     3661 aaaggcaaga ccccaaaaga ggttagggag acgctccacc gagaagacgt cggctacctc
     3721 t
//
LOCUS       DAOPTR010000298         3715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19872_length_3715_cov_2.765847, whole genome shotgun sequence.
ACCESSION   DAOPTR010000298 DAOPTR010000000
VERSION     DAOPTR010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19872_length_3715_cov_2.765847"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..2061
                     /locus_tag="PKI05_06805"
     CDS             175..2061
                     /locus_tag="PKI05_06805"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOA51949.1"
                     /translation="MGRKLVFGTLVCVVIIMADLPMRCAPSGFGELFASDLRQAWESP
                     APHEVQLGGEIGRRIRCTIEENLLKLDLDRDFLQAFRVRNKKDGYVGLGKLLDSIVRL
                     AAHTKDARLIEMKDRIVGELCQLQEPDGYLGIMEPQSRMWKLWDIHEMSYLVYALASN
                     HRFFKEQRSLECGKRLADYIIRNWEAHPEAKPGGGSITVYMAVTGLENAFLLLAEETG
                     ETRYRDFVVNFRQLPEWDARIVVGRHGDIEGHIYAYLCRSIAQLRLSSVYPEERLWRA
                     SRQALDFLLHKEGLVITGECGDHECWHDTQAGTINLGETCATAYLIRWLDELLRREKK
                     SLYGDLMERAIYNGLFAAQSPDGRSIRYYTPFDGPRKYFPSDTYCCPNNYRRIIAELP
                     NFVAYATPEGIAVNLYTEGRVTLHLPSGQRVAVIQRTDYPSDGRVTIALDLGAEESFE
                     TRLRIPKFCDHARIDLPGGESLEAKGGDWAVLKRVWKPGDVVTLNLPMGLRFVKGRRA
                     QVGRVAIMYGPLVFCLNRKDHPDLKDVDLRLLTIVPESLTGPFRDDSVRPGGLACEVK
                     AWSPGAWYPQAQPAYTLRLTEFPDPSGEAIYFHVPNPYDVKFVDDELITPVDVEDISS
                     VAQR"
     gene            complement(2310..3323)
                     /locus_tag="PKI05_06810"
     CDS             complement(2310..3323)
                     /locus_tag="PKI05_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51950.1"
                     /translation="MARYGVGMLAFLAFCILPIGSIWGQSACQPACAPACQPACQPAV
                     TCVQKTIMVPQWVVEMRKVRCIERVPEEREITVNVVKWVPETKMVERTRTQWVLEART
                     KEVKCVTYQPVWKTEVCEYIVMVPKIEKVQGVRKVCKMVPVTETRKVCVDEGHWEERP
                     CEPVCGICRHCHRRVRVVQSAACCPPANACQPACAAPKPVCVCKVWVPNPVVKEIQVT
                     CMKPQWVEEPCEYTKCTWVPEKRTREVKRCVWERQESVKQVTYSVCVPRTVVEKVPVT
                     VCKKVCEPVVKKCTVMVPRCVEKEVPVCVRKMVPTTITVPVRPCGLHHRVALRRAAPC
                     CDP"
BASE COUNT          795 a         1027 c         1020 g          873 t
ORIGIN      
        1 ccttttacgt atcgtccttt caaccgccca tttctcaatt cccggtgtcg cccgggggct
       61 acccgaccga gggatatctt taacgctata ataacagcca agttgacacg cacatttttg
      121 cacaattggc atggcaggaa actcggcgga gcgactcacg agaggagatg cgagatgggt
      181 cgtaaactcg tttttgggac gttggtgtgt gtagtgatca ttatggcgga cctaccgatg
      241 cgatgcgcgc cgtcggggtt cggcgagctt ttcgcgagcg acctcagaca agcttgggaa
      301 tcgccggccc ctcacgaggt gcagcttggt ggtgaaatcg ggcggagaat tcgctgcacc
      361 atcgaggaaa atctgctgaa actcgatctg gaccgggatt ttttacaggc gttccgagtt
      421 cgtaacaaga aggatggata tgtcggcctg ggaaagctgc tcgatagcat tgtgcgatta
      481 gccgctcata caaaagatgc ccgtctgatc gagatgaagg atcgcatcgt tggtgagttg
      541 tgccaattgc aggagccgga tggttacctt gggattatgg aaccccagtc ccgcatgtgg
      601 aagttgtggg acatccacga aatgtcgtac cttgtgtatg ccctcgcctc gaaccatcgt
      661 tttttcaagg agcaacgatc tttggaatgt gggaaacgtt tggcagatta catcatccga
      721 aattgggaag ctcatccgga ggcgaaaccc ggcggtggtt caataacggt ctatatggct
      781 gttactggat tggaaaacgc ctttttactg ttggcggagg aaacgggcga aacgcgttac
      841 cgcgattttg tggtcaattt ccggcagctt cctgaatggg atgcgcggat tgtggttggg
      901 cgccatggtg acatcgaggg gcacatttac gcctaccttt gccgcagtat tgcccaactt
      961 cgactcagtt ctgtttatcc cgaggagcgg ctttggcggg caagtcgcca ggctttggat
     1021 ttcctcctgc acaaagaagg acttgttatt accggtgaat gcggagacca tgagtgctgg
     1081 cacgatacgc aggccggtac gatcaacttg ggggagacct gtgcaacggc gtatttgatc
     1141 cgctggctag acgagctgtt gcgtcgggaa aagaaaagcc tttatggcga cttaatggag
     1201 cgggccattt acaacggtct ttttgccgct cagtcgcccg atggccggtc aattcgctac
     1261 tatacacctt ttgatggtcc tcggaagtat ttcccgtcgg atacgtattg ctgtcccaac
     1321 aattaccggc ggattatcgc ggaattgccg aattttgtgg cctatgcgac tccggagggg
     1381 attgcggtta atctctacac agagggacga gtgacgcttc atcttccgag tgggcagcgg
     1441 gtcgccgtca tccagaggac agactatccc tccgatggtc gcgttaccat cgcgctggat
     1501 ctgggggcgg aagagagttt cgaaacaaga ctccgcattc caaagttctg tgatcatgcc
     1561 aggatcgacc tcccaggagg cgaatcgctc gaagctaaag gtggagactg ggcggttctc
     1621 aaacgagtct ggaagccggg agatgtggtc accctaaatc tgccgatggg gcttcggttt
     1681 gtcaaagggc gacgtgccca agtgggccgt gtcgcgatca tgtatggtcc cctggtgttt
     1741 tgcctcaatc gaaaggacca ccctgacttg aaagacgtag atttgcggct actgacaatt
     1801 gttcccgaaa gtttgactgg tccttttcgt gatgactcag taagacctgg cggattggcg
     1861 tgtgaggtca aagcgtggtc ccccggagcg tggtaccccc aggcgcaacc ggcttacact
     1921 ctgcgactaa ccgagtttcc cgatccctcc ggggaggcaa tctacttcca tgtgccaaac
     1981 ccatacgacg tcaaattcgt ggacgatgaa ttgatcacgc cggttgatgt cgaggatatt
     2041 tcgtccgtag cccagcgttg aaaaatgatc ttggcaactg tgtgtttcat gtgtctcgca
     2101 gagcctttgt ccgtctcgga gccaccgacg aaatctatac ctggcgaagg ggcacaacaa
     2161 acaaaaaacg ccgactcaca cgagtcggcg ccagtgccca tcggttctaa gacgggggtg
     2221 accacgctag gtcaaacgct cctttgacga tcataaggcg atcacaattg cgcgaaaacc
     2281 ttcgtcaaaa acaagcgttt gcctttccct tatggatcgc agcatggggc tgcccgccgc
     2341 agggcaacgc ggtgatgtaa gccgcacggc cgaacgggaa ccgtgatggt cgtgggaacc
     2401 atcttgcgaa cgcaaacggg gacttccttt tccacgcaac ggggaaccat gaccgtgcat
     2461 ttcttcacaa caggctcaca aacctttttg cacacggtga caggaacttt ttcgaccacc
     2521 gtacgaggaa cacatacgct ataggtcacc tgcttgacgg attcctgtcg ctcccaaacg
     2581 cagcgtttga cttccctggt tctcttttcg ggtacccatg tgcatttggt gtattcgcag
     2641 ggttcctcca cccactgggg cttcatgcag gtaacctgga tttccttgac aacaggattt
     2701 ggtacccaca ctttgcagac acaaacgggc ttgggtgcgg cacaggccgg ctgacacgcg
     2761 ttggctggcg ggcaacatgc cgcagactgg accaccctga cccgtcggtg gcaatgtcgg
     2821 caaatgccgc agaccggctc gcaaggccgc tcttcccagt ggccctcgtc cacgcagact
     2881 ttgcgggttt ccgtcacggg taccatcttg cagaccttgc gaactccctg gaccttttcg
     2941 atcttgggca ccatcacgat gtactcacag acctcggtct tccagaccgg ctggtaggtg
     3001 acgcatttca cttccttcgt gcgtgcctcg aggacccatt gtgtcctggt ccgctcgacc
     3061 atcttggttt cggggaccca tttcaccacg ttcaccgtga tttcccgctc ttccggaacc
     3121 cgttcgatac atcgaacctt acgcatttcg accacccatt gcgggaccat gatcgtcttt
     3181 tggacgcagg tcaccgcggg ttggcaagcc ggctgacagg ccggtgcaca cgcgggttga
     3241 cacgccgatt gaccccagat tgagcctatc ggcaaaatgc agaacgcgag aaacgccagc
     3301 atcccaacgc cgtaccgagc catgactcac tcctcctcaa agctagggtt acagtgtggg
     3361 taacatcacc cgatcggaac cctcccgatc taccctgatt gtgtgtcacc cgccgccttc
     3421 gaccttcccc gagccaaagc ttgcgaaaca atgccgcaca ggggaatatt gcttagccgt
     3481 cgccccctcg tcaaatacct caccccaatc gatacatccc attctgtcga taaatttttc
     3541 cccctttgtc cagcttgatg ttatcggagg gtcaagggcg ggtcaagccc ccgcaacgca
     3601 ttacgggccc cggtttttcc ccagcacggc cttcacttga agtccgccat tctattcgcc
     3661 atgctggttc gcgcgagtcg ttttttgtct ttacagtcgc agccaaaaga ggtgc
//
LOCUS       DAOPTR010000299         3712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_19890_length_3712_cov_2.800656, whole genome shotgun sequence.
ACCESSION   DAOPTR010000299 DAOPTR010000000
VERSION     DAOPTR010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19890_length_3712_cov_2.800656"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="PKI05_06815"
     CDS             <1..973
                     /locus_tag="PKI05_06815"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOA51951.1"
                     /translation="DALGPLFVAARQADPASRNWLLSAADQIVAREHGAGRQLSQEVI
                     LKLLEDTQNPPQGRWWAYQWLARQDPAQARQILIRFLNDPASELRRAAIEAELQALGD
                     IQNVPVNDPKNVEIVRKLKQLFDAARDQDQVENLAEMLRKRGVSVDLAGHYGYLRTWW
                     IVGPFDNQQDVGFDAIYPPEGQSPPDFSATYQGKHGPVTWKKVTTPDEHGTFDLNKVL
                     GEEKGVVAYAVAALVSQTEQAAEIRAATPNAIKIWCNGQPLGAFHVYHSGFEDDQYRV
                     VCRLQPGVNWIMIKVCQNEQTQDWAQYWTFRLRVCDELGKGLGRQPD"
     gene            1011..2276
                     /locus_tag="PKI05_06820"
     CDS             1011..2276
                     /locus_tag="PKI05_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOA51952.1"
                     /translation="MIKVRMHIYLAALLVGACCGTWELAQGEDWPAFRGGLAAGVLCG
                     GEDIPPVLSPENQLLWKAPLPGRGPSSPIVVKDKVIVTAAVGYRQNELHVLCFDAKTG
                     KQEWDFRFWATGSTVCNPFGGVAASTPVSDGRRIVALFSSNDLVCLDMEGRPLWFRGL
                     GFERPYLRNDVGMASSPVIEGDVVIVQAESLLDAIVCGLDLKTGQTVWLIDRPKQALW
                     CTPLVVRCPSPSERTPETFVLLQSRKDFQAVCPYTGEVAFAYDHWCDTVSSAAAYGDL
                     VALPAAGIHALRLSSSPARFEQLWISDRLRCGNTSPLVWNNKIYLVKSPNIFACADGK
                     TGDILRQVRLGGSFWASPLLVGRYIYAVNYEGTVFVMALDDAGLPSLAGQWDLESGVL
                     ASPASDGRQLYFRTNQFLYAFRLGDSGKE"
     gene            2379..3668
                     /gene="aroA"
                     /locus_tag="PKI05_06825"
     CDS             2379..3668
                     /gene="aroA"
                     /locus_tag="PKI05_06825"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOA51953.1"
                     /translation="MMAEAGDFVEIPLSGPVNATVQPPGSKSITNRALICAALADGPS
                     LLQGALDSEDTQVMVRALQSLGVAVRHDTKQREIAVEGTRGVIPARQADLFLANSGTS
                     LRFLTAICTLGEGQFRLDGTPRMRERPIQDLVDALNRLGANVRTELPTGCPPVIVEAH
                     GLPGGETEICGEISSQFFSGLLMAAPYSRCDVTLLVRGDLVSKPYVEMTLAVMKAFGV
                     TGENEAFQRLSVRSGQMYRGRTYAIEPDASAATYFFAAAAVTQGRITVTGLGENSLQG
                     DVRFVDCLAAMGCEVTMGDDQITVEGRPLRGISVDMNAISDTVPTLAVVALFAEGPTL
                     IRGVKHIRHKETDRIHALALELRRLGAQVEEFEDGLRIIPASYHPAEIHTYQDHRMAM
                     SFAIAGLRIPGIKILDPGCTAKTYPEFFEDLRAIIRP"
BASE COUNT          729 a         1072 c         1103 g          808 t
ORIGIN      
        1 cgatgccttg gggcccttat ttgtggctgc ccgccaggct gatcccgcat cccgcaattg
       61 gctcctttcg gcggcggatc agattgtagc gagggaacac ggcgctgggc gacaactctc
      121 gcaggaagtg atccttaaac tgctcgagga tacgcaaaat cctccgcaag gtcgctggtg
      181 ggcgtaccaa tggctcgctc ggcaagaccc cgcccaggct cggcagatcc tgatccgatt
      241 cctcaatgat cccgccagcg agttgcgacg tgcggccatt gaggcggagc ttcaagcgct
      301 aggtgatatt caaaacgttc cggtaaatga tcccaagaac gtcgagattg tacggaagct
      361 caaacaactg tttgacgctg cccgggacca agatcaagtt gaaaacctcg ccgaaatgct
      421 gcgcaaacgg ggcgtgtccg tggacctggc cggtcattac ggatacctgc gcacgtggtg
      481 gattgtcggg ccgtttgata atcagcaaga cgttggtttt gacgccatct atccgccgga
      541 ggggcaatcc ccgccagact tctccgccac gtatcaggga aaacatgggc cagtaacctg
      601 gaaaaaagtg acgacccctg acgaacatgg aacctttgat ctcaacaaag tcctcggcga
      661 ggaaaagggt gttgttgcct acgcggtagc cgccctcgtt tctcagacgg aacaagccgc
      721 ggaaattcgc gcggcaacgc cgaacgccat caaaatctgg tgcaacggcc aacccttggg
      781 ggccttccat gtgtatcact ccggttttga ggacgatcaa taccgcgtgg tctgccgcct
      841 gcaacctggc gtcaattgga tcatgatcaa agtctgtcag aacgaacaga cgcaagattg
      901 ggcacaatat tggacgtttc ggctgcgggt ctgcgacgag ctcggcaagg gacttggtcg
      961 tcaacccgat tgagcggcct gctttttgaa catggtggga aggttccgca atgatcaagg
     1021 ttcggatgca tatctacctt gcggccttgc tggtcggcgc ctgctgtggc acgtgggaat
     1081 tggcccaggg ggaggactgg ccggcgtttc gtggcggtct agcggccggc gtgctctgcg
     1141 ggggtgaaga catcccgccg gtactctccc ccgagaacca acttttgtgg aaggccccac
     1201 tgccgggccg cggtccgtcc tcgcccatcg tggtgaagga taaggtgatt gtcacggctg
     1261 ctgtgggtta tcgtcaaaac gagttgcacg tcctctgctt cgatgcaaaa acgggcaagc
     1321 aggagtggga ttttcgcttt tgggcgactg gttccaccgt gtgcaatccc ttcggcggtg
     1381 tggcggcctc gacgccggtc tcagatggtc ggcggattgt ggccctcttt tcgtctaatg
     1441 acctcgtctg cctcgatatg gagggacgtc ccctctggtt ccgtgggctg ggatttgaac
     1501 gcccctacct ccgcaatgat gtgggaatgg cctcctctcc agtgattgag ggtgatgtgg
     1561 ttatcgtcca ggcagaaagc ctcctcgatg ccattgtttg cggactggat ctaaagacag
     1621 gacagaccgt ttggctgatt gatcgtccca agcaagccct ttggtgcacg ccgttggtcg
     1681 tgcgctgccc atcgccctcc gagcgaaccc ctgagacttt cgtccttctc caatcacgaa
     1741 aggactttca agccgtctgc ccttacaccg gcgaggtggc ctttgcctat gatcattggt
     1801 gcgacaccgt atcctccgca gcggcttatg gtgatttggt tgccttgccc gcggcgggca
     1861 tccacgcctt gcggctatct tcttccccgg cgaggttcga gcaactttgg atttccgatc
     1921 gcctccgctg tggaaatacc agccctcttg tctggaacaa caagatatac ctggtcaagt
     1981 cccccaatat cttcgcctgt gctgatggaa agaccggcga cattctgcgg caagtccgcc
     2041 tagggggtag cttttgggcc tctccgcttt tggttggccg atacatctac gccgtcaatt
     2101 acgagggtac ggtgttcgtc atggcactgg atgacgcggg gcttcccagc ctcgccgggc
     2161 aatgggacct cgaatcaggc gtgctggcct cacccgccag cgatggacgg caactctatt
     2221 tccggacaaa tcagttctta tacgctttcc gattggggga ttccggaaaa gaataaagac
     2281 acgggctact gggcaatgtt ctcggatgcc gattggtttt tggcaacaga tctcccggga
     2341 tcttcaacga ttgttgacca aacattgcta gagaaagcat gatggccgaa gcaggcgatt
     2401 tcgtcgaaat ccccttgtct ggaccggtta acgccaccgt tcagccaccg ggctccaaga
     2461 gtatcaccaa ccgtgcgctg atctgtgcgg cgttggccga cgggccgtcc ctcttgcaag
     2521 gggcgttgga tagtgaagat acgcaggtga tggtgcgggc attgcagagc ttaggcgtag
     2581 cggtccgcca cgataccaag cagcgggaaa tcgcggtgga aggaacccgc ggcgtgatcc
     2641 ccgctcgcca agccgatctc tttctcgcca acagtggcac cagccttcgc tttttgacgg
     2701 ctatctgcac cttgggagag ggtcaatttc gactggatgg gacgccccgc atgagagaac
     2761 ggcccatcca ggaccttgtg gatgccctta atcggctggg ggctaacgtg cgaacagagc
     2821 ttcccacggg atgtccgccc gtcattgtcg aagcccatgg tctgccggga ggagagacgg
     2881 agatttgtgg ggaaatctct agtcaattct tcagtggact gttgatggcc gcgccttact
     2941 cccgctgcga tgtgacgctc ctcgtgcggg gtgatttggt ttcaaagccc tacgtggaaa
     3001 tgacgctcgc cgtgatgaag gcctttgggg tcaccggcga aaatgaggcc tttcagcggc
     3061 tttccgtgcg atctggtcaa atgtatcgcg gccgaaccta cgccattgag cccgatgcct
     3121 cggcagccac gtacttcttc gccgccgcgg ccgtcaccca gggccggatc accgtaaccg
     3181 gcctgggcga gaatagcctt cagggtgatg tccgctttgt cgattgcctg gccgcgatgg
     3241 gctgcgaggt gacaatgggc gatgaccaga tcactgtgga aggccgcccc ctccgcggca
     3301 tctccgtgga tatgaacgcc atcagcgata ccgtgcctac tcttgccgtc gtggccctct
     3361 tcgctgaggg gccgacgctc attcgcgggg taaagcacat ccgccacaaa gagaccgacc
     3421 gcatccacgc cctggccctt gaactgcgac gcctgggggc ccaggtggaa gaattcgagg
     3481 acggactgag aatcatcccc gcctcctatc atccggcgga aatccatacc taccaggatc
     3541 accgcatggc catgagcttc gccatcgctg gattacgcat tcccgggata aaaatcctcg
     3601 atccgggttg cacggccaag acgtatcccg agtttttcga ggacctgcgg gcgatcatcc
     3661 ggccctagcg atgtcacgaa cggccggggg cacggtattc ctcgggatag at
//
LOCUS       DAOPTR010000300         3684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_20065_length_3684_cov_3.466244, whole genome shotgun sequence.
ACCESSION   DAOPTR010000300 DAOPTR010000000
VERSION     DAOPTR010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20065_length_3684_cov_3.466244"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1044)
                     /locus_tag="PKI05_06830"
     CDS             complement(40..1044)
                     /locus_tag="PKI05_06830"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOA51954.1"
                     /translation="MMNMKNLLVALIFICLPNCALAENWATKMFETLEHDFGVVARGA
                     KAEYRFVFKNLYMEDVHVQSARSSCGCTLVRVENPSLKTYETGAIVATVNSRAFVGAR
                     SATITVVFDQPYYAEVQLRVRSYIRSDIVFDPESVNFGTIDCGSPAERTITIRYSGSR
                     SWRITDVVSPLPYLTATLSEPSILGSETVYRLTVKLGEEAPPGTLADHLILKTNDRTS
                     DAVPLRVEGTIVSPISVTPSTLFLGRMKPGEKVSKQLVVRSKQPIRISKVDCEPCNGV
                     LEADLSRAGNPATVHVIPITYTAGEELGQIVANIRIHTDAPSVNESSLLARAVAVVQK
                     "
     gene            1198..2032
                     /locus_tag="PKI05_06835"
     CDS             join(1198..1557,1559..2032)
                     /locus_tag="PKI05_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909840.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HOA51955.1"
                     /translation="MGARAERISANWRIPDALWKRMEPLLPVRKRRRRYPGRKPLAWR
                     QVIDGIFYVLRTGCQWKAAPREFGSGSSLHRYFQKLVACGVFARLWKIALEEYDELRG
                     IQWEWQCLDGATSKAPLGGEKTGPNPTDRAKSGTKRSVLTDGAGVPLGVAVGGANTHD
                     KRLVKETLESVPVERPKPTPRKKQNLCADKGYDYPDVRQLLHDWGYTAHIKSRGEEEC
                     EGKEIPGYRARRWVVERTQSWLNRFRRLLIRWEKKVENYLALLHFACAWITFRAAGIL
                     G"
     gene            2392..2718
                     /gene="trxA"
                     /locus_tag="PKI05_06840"
     CDS             2392..2718
                     /gene="trxA"
                     /locus_tag="PKI05_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOA51956.1"
                     /translation="MGKAISLTEQSFDQEVLQSDVPVLVDFWAPWCGPCRMIAPTIEQ
                     LAEEFAGQAKVCKLNVDESTAIAARYSVTSIPTIIIFKNGQVVDRYVGAQSKERLRDA
                     LVEATR"
     gene            complement(2807..>3684)
                     /locus_tag="PKI05_06845"
     CDS             complement(2807..>3684)
                     /locus_tag="PKI05_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA51957.1"
                     /translation="IAKCQELAKTFSRTKRIFQAGTQRRSVPNFIYAVELARSGKLGK
                     IHTLYASVYEPQLDNSWLPREQTPPRDVVDWNLWLGPAPWRPYNQKYVQGGWRGYFDF
                     DSGARLLDWGAHTVDLCQWAIGADNTVPVEYEPMGDKIVCTYASGVKLILEFLKTPFG
                     DRSPKFITELGTCPVRFVGEEGWVETGDNGGIEVHPDTLKKEVPEAITRVRGLDVSAH
                     ARNFFDCVKSRAKTVTNEDVMRHSHVACHAAAIAWILDRKLRFDPKTEKFIGDEEANL
                     LCARPERNPFTEHGV"
BASE COUNT          831 a          950 c         1086 g          817 t
ORIGIN      
        1 gattgggtcc aagtctggaa ttaggtctaa agcaagtcgc tacttttgga ccacagctac
       61 ggccctcgcc aagagtgagg attcatttac ggagggggcg tccgtatgaa tccggatgtt
      121 ggccacaatc tggcccaact cctcaccggc ggtatatgtg atagggatga catgcacggt
      181 ggccggattg ccggcacgtg agagatcggc ctccagaacg ccgttgcacg gttcgcaatc
      241 gaccttcgaa atgcgaatcg gttgttttga acgcacaacc agttgtttgc tgactttttc
      301 gccgggtttc attcgtccga gaaagagggt gctgggtgtc acagaaatgg gcgacacaat
      361 ggtcccttca actcgcaagg ggaccgcatc tgacgtccga tcgtttgtct tcagaatcag
      421 gtggtcggcc agcgtacctg gcggtgcctc ttctcccaac ttcacggtga gacgataaac
      481 tgtctccgaa ccaagaatcg atggttcgga cagtgtggcc gtcaaatacg gtagcggcga
      541 aactacatcg gtgatccgcc aacttcggct tccagagtac ctgatggtaa tagtccgctc
      601 agcgggactt ccacagtcaa tggttccaaa attgaccgat tccgggtcaa aaacaatatc
      661 gctccggatg tagctcctta cccggagctg aacttcggcg tagtagggct ggtcaaaaac
      721 cacagtgatc gtagcgctac gggccccaac gaatgcgcgg gagttgacgg ttgccacaat
      781 ggcaccagtc tcgtaagtct tgagggaagg attctcgaca cgaaccaacg tgcagccgca
      841 cgaagatcgg gcactctgga cgtggacgtc ctccatgtag agattcttga aaacgaagcg
      901 gtactccgct ttggccccgc gagcaaccac tccaaagtca tgctcgaggg tttcaaacat
      961 tttggtcgcc caattctccg caagggcgca attaggcaag cagatgaata tcagcgcgac
     1021 aaggaggttc ttcatgttca tcatgaacgt gtcctttcgg agggctaatt caattatacg
     1081 ccagtgaaag tttctcgccg gggcttagag cctatcccaa aatgacgttt acggaaggag
     1141 gactttgggt tatactatca gacgcagtgg tagttaggta aggcaaggag gtctgggatg
     1201 ggtgcaagag cggagcgaat ctcggcgaat tggagaattc ccgatgcttt gtggaagcgc
     1261 atggaacctc tgcttccggt acggaagcga cgacgccggt atccggggcg taagccattg
     1321 gcatggcgac aagtgatcga tgggattttc tatgtgctgc gtacaggctg ccagtggaaa
     1381 gcggcgcccc gcgagtttgg ttccggcagt agtttgcacc gttactttca gaagcttgtg
     1441 gcgtgtgggg tgtttgctcg tctctggaag atagcactgg aggagtacga tgagttgcgg
     1501 ggcatccagt gggaatggca gtgtctcgac ggggcgacga gcaaggcccc gctgggaggg
     1561 ggaaaaaacg gggccgaatc ccacggatcg ggccaagtcg ggcacgaagc gatccgtgct
     1621 taccgatggc gccggtgtcc ccctgggggt ggcggtcggt ggtgccaaca cgcacgacaa
     1681 gcggttggtt aaggagacgt tggagagtgt tccggtggag cgacccaaac ccacgcctcg
     1741 caagaaacag aacctatgtg ccgacaaggg atatgactat cctgacgtgc gacagttgct
     1801 ccacgactgg ggttatacgg cccacatcaa gtcgcgaggc gaggaggaat gcgaggggaa
     1861 ggagattcct ggctaccgtg cgcggcgttg ggtggtcgag cggacgcaat cctggcttaa
     1921 tcgatttcgc aggcttctga ttcgctggga aaagaaggtg gaaaactacc ttgccctcct
     1981 tcatttcgcc tgcgcgtgga tcactttccg cgccgccggc attttgggat aggctcttag
     2041 tactgtccga aggttttgct gtcagaaacg ccaaggggga tcgcgtaggt gagactacgg
     2101 taggtcaaag caattcattg tctgacgggg gcaccgagtc cggccccgtt cgaaaaaaca
     2161 tagattggaa aaaacctgaa atggccaacg aggggtgcga aaaatcagag gtctaagacc
     2221 cttttggggc ggggtttacc gattatgatt gattttcggc gaaaaggctt ggggagttgc
     2281 ccccgttgac cgaggcttta taatcgcaag aggtatggtt taagtatttg cgacacagta
     2341 acatcgaatt gaactaggga gcttggcccc caaattagga gcaactgccc gatgggaaaa
     2401 gccatttcat tgactgagca gagtttcgat caagaagtct tgcaatcgga cgttccggtt
     2461 ctcgtcgatt tctgggcacc ctggtgcggg ccctgccgga tgatcgcccc gactatcgaa
     2521 caattggctg aggagtttgc cggccaggca aaggtctgca agcttaatgt tgacgaaagc
     2581 accgccattg ctgcgcggta cagcgttacc agcatcccga cgatcattat tttcaagaac
     2641 ggccaagtcg ttgaccgtta tgtgggtgcg cagtccaaag agcgactacg ggatgctctt
     2701 gtcgaggcca cgcgttgacg gtctttgcca tacaccccct gtgcgcgaac tgccagggag
     2761 taatcccggt tctgtcgtcg ttgccagacc catcgtggga ttgtcactag accccgtgct
     2821 ctgtgaaggg gtttcgttcc gggcgtgcgc agaggagatt ggcctcctcg tcgccgatga
     2881 atttttccgt cttaggatcg aaacggagtt tccggtctag aatccaagcg atcgcggcgg
     2941 cgtggcacgc aacgtgcgaa tgtcgcatga catcctcatt ggtgaccgtc ttggcacgcg
     3001 atttgacaca atcaaagaag tttcgcgcat gtgcggagac gtccaggcct cgaacgcgag
     3061 tgatggcttc gggtacttct tttttcaagg tgtcagggtg gacctcgatc ccgccattgt
     3121 cacccgtttc cacccaacct tcctcgccga caaatcggac cggacacgtg cccaactctg
     3181 taataaactt cggagaacga tcaccaaatg gtgttttcag gaactccagg atcagtttga
     3241 cgccactggc ataggtgcaa acaattttgt cacccattgg ctcatattcc accggcacgg
     3301 tattgtcggc cccgatcgcc cattggcaca aatcgacggt gtgtgcgccc cagtccaaca
     3361 gtcgggcacc ggagtcgaaa tcgaaatagc cccgccaacc gccctggaca tacttttggt
     3421 tgtacggccg ccaaggtgcc gggcccagcc aaaggttcca atcgaccaca tcacggggcg
     3481 gagtctgttc ccgcggcagc caactattgt ccagttgggg ttcgtaaacc gaggcataca
     3541 acgtatggat ctttcctagc ttgccgctgc gagccaactc aacagcataa ataaagttgg
     3601 ggacgctccg tcgctgcgtg ccggcctgaa agattcgctt tgtccgcgaa aaagtcttgg
     3661 ccagttcctg gcactttgca atcg
//
LOCUS       DAOPTR010000301         3682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_20076_length_3682_cov_4.322029, whole genome shotgun sequence.
ACCESSION   DAOPTR010000301 DAOPTR010000000
VERSION     DAOPTR010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20076_length_3682_cov_4.322029"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(303..719)
                     /locus_tag="PKI05_06850"
     CDS             complement(303..719)
                     /locus_tag="PKI05_06850"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOA51958.1"
                     /translation="MTLVWDTTMSTGIPRIDAQHQELIRKLNGLLHAMQKGRGKEEIE
                     KLLDFLAEYVIKHFSDEEAEMERVNCPAAIANRIAHQHFIKRFTEFRERFKKEGASPR
                     IVIDVQKTLMDWVIMHIRGVDTKLAASVTRKAEART"
     gene            complement(1039..1455)
                     /locus_tag="PKI05_06855"
     CDS             complement(1039..1455)
                     /locus_tag="PKI05_06855"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HOA51959.1"
                     /translation="MAVAWDQSLSTGVAKIDRQHQELIANINSLLEAMRSGRGRDEIG
                     KLIAFLSDYVVKHFADEEAEMDRVKCPVAVANRIAHQKFMTRFKEIQERFNKDGATPK
                     LVIDVQQAVLDWFIAHIRGIDTKLASSAKAKVGVGS"
     gene            1752..3314
                     /locus_tag="PKI05_06860"
     CDS             1752..3314
                     /locus_tag="PKI05_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51960.1"
                     /translation="MVRRFRLRFHKVWLSLLLIAGGCAATAGHLGGPFSPGAPQVTQS
                     SPGASESPNPNSLTTATPAAPGSASPDISKPASPGSAQSASSAGSAGPGSSSRESAAG
                     NNIVSVDLLVSELEAEGLLDAESKAQLTADLCRTPPHLWEGIVAAFRASMQLRKQKSD
                     QGQLQSSDVFIEDKDVRPAAGHTSTGSLPPQDSRASSQTGLTSVVQGAIPDDRARLPR
                     LASMDRFSDIRKEQASSQMDTQVLSEPEARPDSPIASQIARSLPARPSPPGGQETKSD
                     AFKSPSPSSEEAAGTWDYWVTRAVRDLEDQQTRSTREEIQLRLLRLALIRREGALEPI
                     PGLSPSLQDFWGQTLFGLLLLEDTQLHSDQQLRLVEARRHLENGIRRLAEECPLEVTG
                     LAFVTSVQSWGVYDAFEQYEFLPGQKVLLYAEIENLASESTAKGYRTAWRSSYQILDA
                     TGKQIASYEYAPNEEYCRRPRRDFFIGCELSFPTDAPPGRYTLRLTVVDLIKQRVGQG
                     TVDFTLRSRQAK"
     gene            complement(3460..>3682)
                     /locus_tag="PKI05_06865"
     CDS             complement(3460..>3682)
                     /locus_tag="PKI05_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOA51961.1"
                     /translation="DIVMPGTDGLYALDGILALNPNAKVVVVSALNQTKLISDAIRKG
                     AQDFIAKPFLPEQLLDTVSSCLCESSVPA"
BASE COUNT          844 a         1044 c          925 g          869 t
ORIGIN      
        1 gatgctccat cacaagaccg atcgccgagc cgccgttact ttaatcctac caaatcttcc
       61 cattcatcca agatgaatgt gcggttccat ccacctgcta acgttagcca gattaacaaa
      121 aataccgctt acagaagagc ggtaaacagc gcaaaatctg acagtcccgg cgacgttgcg
      181 gctgccgaga ggaggaggac gtgaacgtcc tcgcaatcag caaacaccgg gggctatcct
      241 gctgacgagt gcgtactcga ttggctgttt cggctcgtcg aacgataacg gtatcctgat
      301 gttcaggtcc gagcttcggc ttttcgtgtt acgctggcag cgagcttcgt atccactccc
      361 cgaatgtgca tgatcaccca gtccatcaag gtcttttgaa cgtcgatcac gatccgggga
      421 ctagctcctt cttttttgaa ccgttctcta aactcggtaa atcgtttgat gaaatgttga
      481 tgcgcaatgc gattggcgat ggctgccgga caattgaccc gttccatctc tgcttcttcg
      541 tcggagaagt gcttaatcac gtactcggcc agaaagtcga ggagtttctc gatttcttcc
      601 ttccctcggc ctttttgcat agcgtgcaat agcccgttta gcttgcggat caactcttgg
      661 tgctgagcat cgattctcgg aatgcccgtt gacattgtcg tgtcccaaac aagcgtcata
      721 gcaaaaactc ctcaatagtc tggaaaacga tctttctctt tccctaaaac ctgcgagtga
      781 accgacccac cgaagcttgt cggactcttg cactcacaag caaaacactc tcgcactgaa
      841 ccacccctgg ctccttgttc taaagccagg tttgagttgg tgataactcg tttaaaatgc
      901 gtgccgggcc aggattcgtg gtgcaacata caccgaagcg tcgttcttgg tgcgaaatcg
      961 cacacttacg cggcaacgga tcgaatttgg aaatttctgg ctcttttcgg aaatcgccaa
     1021 aggaatccaa gttgtggatc aagaaccaac tcccacctta gccttcgcgc tactagcaag
     1081 cttcgtgtca ataccgcgaa tgtgggcaat gaaccagtca agcactgctt gctggacgtc
     1141 tatgaccaat ttgggcgtgg caccatcctt gttgaagcgt tcctggatct ctttaaatcg
     1201 tgtcatgaac ttttgatgtg cgatccggtt ggccacggcc acggggcatt tgacgcggtc
     1261 catctccgcc tcttcatcgg cgaagtgctt gacgacgtag tcacttaaga acgcgatcaa
     1321 ctttccgatc tcatctctgc cgcggccact ccgcattgct tcgagaagcg aattgatgtt
     1381 cgcaataagc tcctggtgct ggcgatcgat ttttgccacg ccggtagata aactttggtc
     1441 ccaagcgacg gccatagcgg gtccccctat gttcttagtt ttttgccaat atcaccaaac
     1501 cacacaaacc tctcacgtac accatctacc ctaacttagc tcgcaaattg ccctcgtgag
     1561 gcgaaacacg gcgcggaacc atcctaatga gtggcaatgc accccactgc cttgtgcata
     1621 ggccgaaacc caatcttctc ggcgaacttg cccctttttc tgtcgtttgg taacatccac
     1681 cgccccaaaa ccacaaaacc ggaggaaaac ccgtctctcc tcgggcacgg cggttggaac
     1741 caagaggttt tatggtcagg cgttttcgct tgcgatttca caaagtctgg ttgagtctgc
     1801 tcctgatagc aggggggtgt gccgccactg cgggacacct cggtggtcct ttttcgccag
     1861 gagcgcctca agttacgcag tcttcaccgg gcgcatcaga aagtcctaac cccaattcac
     1921 tgacaactgc caccccagcg gccccgggtt cggcctcacc agacatttcc aaaccggcat
     1981 cgccaggttc agcccaatca gcatcctctg cgggttcagc aggtcccggc tcaagttcgc
     2041 gggaatcagc cgctggtaac aacattgttt cggttgatct gctggtatct gagcttgagg
     2101 ctgaaggtct gctcgatgcc gaatcgaagg cccaacttac ggcggacctc tgtcgtactc
     2161 cgccacacct ttgggaagga attgtggctg catttcgcgc cagtatgcag ttgaggaaac
     2221 aaaaaagtga ccagggccag ctccagtcga gcgatgtatt catcgaggac aaagacgtcc
     2281 gcccagccgc cggccacacc tcgaccggat cattgccgcc ccaagactcc cgagcaagct
     2341 cgcaaacagg gttaacatct gtcgtccagg gggccatccc tgatgatcgc gcgaggcttc
     2401 ctaggcttgc atctatggat cgtttctcgg acattcgcaa agagcaagcc tcatcgcaga
     2461 tggacaccca agtcttgtcc gaacccgaag ctaggcctga ttcgccgata gcttcccaaa
     2521 ttgcccgtag cttgccggct cgcccatcgc cgccaggtgg tcaagaaacg aagtctgatg
     2581 catttaaatc gccgtctcct tctagtgaag aagcggccgg aacgtgggac tactgggtaa
     2641 ccagagcagt tcgggacctc gaggatcaac aaacacgcag cacaagggaa gaaatacagc
     2701 ttcgtctctt gcgcttggcg ttaatccgtc gtgagggtgc tctagaaccc ataccgggct
     2761 tgagcccatc tttgcaggat ttctggggtc aaacactttt tggactcctg ttacttgaag
     2821 atactcagtt gcattcggac caacagctcc ggttggtaga ggcacgccgt cacttagaaa
     2881 acggcattcg acgcttggca gaggaatgcc cgctcgaggt gaccgggttg gcgtttgtca
     2941 cttccgtcca gagctggggg gtgtacgacg ccttcgaaca gtatgagttt ttgcccggtc
     3001 aaaaggtcct cttgtacgcc gagatcgaga atcttgccag cgaatccacg gcgaagggct
     3061 accgaacggc ttggcgaagt agttaccaaa tcctcgatgc cacaggcaag cagatcgcta
     3121 gttatgagta tgctcccaac gaagagtact gccggcgacc acgtcgggat tttttcattg
     3181 gatgtgaatt gtcctttccc accgacgcac cgcctggccg gtacacgctg cggctcaccg
     3241 ttgtcgattt gatcaagcaa cgagtagggc aggggacggt ggactttact ttgcgatctc
     3301 gccaggcgaa atgacggcct acgcttcctg ccgtaagaat gatcgctgat caccaacttg
     3361 accgtcagtg agctgtccgc tttaacgcgt cggaaagcaa tcatgcgtca cgaaacgggg
     3421 aaccgtttcc ttcggtgatt tattttctag ataaaaggct catgccggaa cggacgattc
     3481 gcaaagacaa ctgctcacag tatccagcaa ttgctcgggc aagaacggtt tcgcaatgaa
     3541 gtcctgcgca cctttccgga tggcatccga gatgagtttc gtttgattga gcgcactgac
     3601 aaccactact ttagcgttgg gattgagggc caaaataccg tctaacgcat aaagaccgtc
     3661 cgtgcccggc atgacaatgt cc
//
LOCUS       DAOPTR010000302         3679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_20111_length_3679_cov_3.213024, whole genome shotgun sequence.
ACCESSION   DAOPTR010000302 DAOPTR010000000
VERSION     DAOPTR010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20111_length_3679_cov_3.213024"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PKI05_06870"
     CDS             complement(<1..778)
                     /locus_tag="PKI05_06870"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA51962.1"
                     /translation="MRPGLSKLRYRMRWDRGPEAFRMGIDLLAAALLITICLSGAVFA
                     ADGLDTPNIVVILADDLGYGDVSSYNPDRGKIPTPNIDRLAKEGMRFTDGHSSSGVCS
                     PSRYTLLTGRYHWRTWLQAGIVGVFGEPLIVPDRMTIGTLAKARGYRTACFGKWHLGW
                     DWPIPAEEMKYFRVARNQKETPPPTKEQVEAWQRVFSQRIPGGPTERGFDEYFSTDVP
                     NWPPFCFIKNDRTVGIPSVLLPDRFLGAEPRMASLPGPALP"
     gene            complement(1086..1406)
                     /locus_tag="PKI05_06875"
     CDS             complement(1086..1406)
                     /locus_tag="PKI05_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51963.1"
                     /translation="MLPFTNGFGNVLDVRQRSCGRVETRRIVWRLKAQRLPHAQRDSP
                     FTRRVWKVIPLGSSPRRATRCPALGNHEASIANALETTARPHTSRMVVVKPAALGYFL
                     LQAN"
     gene            1664..1795
                     /locus_tag="PKI05_06880"
     CDS             1664..1795
                     /locus_tag="PKI05_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51964.1"
                     /translation="MTADKGLPRKSRRIVRRPATRFPHLLKRMRQRLLDRPKKGRAT"
     gene            1861..>3679
                     /locus_tag="PKI05_06885"
     CDS             1861..>3679
                     /locus_tag="PKI05_06885"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HOA51965.1"
                     /translation="MGYRTLRECVKDLLATRQMIRIDVPVDPDLEAAEIQRRVFRAGG
                     PALYFASVRGSRFPMVSNLFGTMERIRYIFRDTLDQLSKLVSLGLDPSDWLRRPRLFL
                     KVPWWGWKAWPKVVSGGPCLKHEIRLQQLPKVRSWPNDGGAYITLPIVYTEDPTQPGF
                     AHSNLGMYRIQITGGKYDPECEVGLHYQIHRGIGIHHARAIEAKQKLRVNVFVGGAPA
                     MTVAAVMPLPEGMSELFFAGLLGGHRIPMIRRKGELPIYAEADFVLTGYIEPKKLLPE
                     GPFGDHLGYYSLVHDFPVMHVEHVYHRPNPIWPFTVVGRPPQEDSMFAQLIHELVGPV
                     IPKAIPGVRAVHAVDAAGVHPLLLAIGSERYTPYDERKRPQELLTLANALLGHGQLSL
                     TKYLFIVAGEDNPDLDVHDVDDFFRHVLERVDWRRDVHFQTCTTVDTLDYTGGALNQG
                     SKVTIAAVGKPRRTLPVALDSRIKIPEDLGFSDPRVMLPGILVIQGPPYRRNEKGEDE
                     SIRQFCARYSRHDAVNLFPLIVIVDDSEFAARTLNNFLWTTFTRSDPAGDIHGIEEFV
                     SKKHWGCHGSLVIDARAKPEHPPPLVEDPEVVRRVEALAA"
BASE COUNT          805 a         1055 c         1024 g          795 t
ORIGIN      
        1 cgggcaaagc gggtcccggg aggctggcca tccgtggttc ggcgccgaga aaacggtcgg
       61 gaagcagcac gcttgggatt cccaccgtcc gatcgttttt gatgaaacaa aacggcggcc
      121 agttcggcac atcggtgcta aagtactcgt caaatccccg ttccgttggg ccacccggta
      181 ttcgttggga aaacaccctc tgccaagctt ctacctgttc cttggttggt ggtggcgttt
      241 ctttctggtt gcgggcaaca cgaaaatact tcatctcctc ggcagggata ggccaatccc
      301 agcccaaatg ccacttacca aaacaggccg tgcggtaccc acgggccttg gccaacgtgc
      361 cgatcgtcat gcgatcggga acgatcaacg gctctccaaa cacccccaca atcccggctt
      421 gcagccacgt acgccaatga tagcgccccg tcagcagcgt gtaccgagag ggagaacaca
      481 cgccactgct agaatgccca tctgtaaacc gcatcccctc tttggcaagc cgatcgatgt
      541 tgggggtcgg aatctttcct cggtccgggt tatagctgga cacatctccg tagccgaggt
      601 cgtccgcaag aatcaccaca atatttggag tatcgagccc atcggcggca aagacggccc
      661 cggacaaaca gatcgttatc aggagagcgg cggcgagaag gtcaatgccc attcggaagg
      721 cctcgggccc tctatcccac cgcatacgat accgcaactt cgacaaaccc ggcctcatgg
      781 cgctacctcc ccagaaatgc ctattccaaa atggtcaaga aattcgccta ttctgtttca
      841 agcttctctc accacacaaa ggcccaccgc atctcatcac gcagcggaac caacatgatt
      901 tgggccgcgt ttacgtaatc ccgctgacgg ccctcttgaa ggagcccccc cgggccgaat
      961 ggtaattccc ctcaaccttt accgaaaaca ccgatctgct tgaggcatgg ttcagtgttg
     1021 ccaacagcat atccaaacgg atacgcaaaa taaagagacc ggggttttcg cctgtggttg
     1081 atccttcagt tggcctgtaa caaaaagtat cccaacgccg ccggctttac gacgaccata
     1141 cgcgatgtat ggggacgcgc cgtggtctcc agtgcgttag cgattgatgc ctcatgattt
     1201 cccagggcag gacaacgcgt ggctctccgg ggtgaggaac ctaagggaat cactttccat
     1261 acccgacgtg tgaacgggct atcgcgctga gcgtgcggca aacgctgggc ttttaaccgc
     1321 caaacaattc gccgcgtttc cacgcgcccg cacgaccgct gtctcacatc cagcacgttc
     1381 ccgaacccat ttgtgaaggg caacatgggg ttttctcctt agatcgggac ggagattttc
     1441 gtggccgcac ggcgaagtgt ttgatgagtt gtatctcact cgcttcgccg cgcatgaatg
     1501 tgtccgtccc agtacaataa aagtgcaggg tagcagggga gaattggggt ccaagaatga
     1561 tggttttctg aacacatccg acgatcatgg gccaacggaa accctgcgaa cgaggtgtgc
     1621 gtccaaacat ctgtggagca tgttttttgc aagggtcacc atcatgacgg cagacaaagg
     1681 attaccacga aagtcacggc gaattgtccg tcgtccggca acgcgatttc cccatctttt
     1741 gaaacgcatg cggcagcgat tgttggaccg ccccaagaag ggacgagcaa cctgaggcaa
     1801 cgcgtgagcg tgtgatggtg cgttgagcaa cttggcggca acttcggcaa aggatgatcc
     1861 atgggatatc gaacactgcg cgagtgtgtc aaagatttgc tagcaacccg gcagatgatc
     1921 cgcatcgacg tgccagttga ccctgatctt gaggccgccg agatccagcg gcgcgtcttc
     1981 cgtgcggggg ggccggcgct gtacttcgcc tcggtgcgag ggtcgcgctt ccccatggtg
     2041 agtaaccttt ttgggacgat ggagcgaatc cgctatatct ttcgcgatac gctggatcag
     2101 ttgtccaaac tcgtgtcgct gggcttggat ccctccgact ggttgcggcg accaaggctt
     2161 tttctgaagg tgccgtggtg gggatggaag gcctggccga aggtcgttag cggcggccct
     2221 tgcttgaaac atgaaatccg attgcagcaa cttcccaagg tccgttcctg gcccaatgac
     2281 ggcggggcgt acatcaccct cccgatcgtt tacacagagg accccacgca accaggtttc
     2341 gcccattcga atctggggat gtacaggatt caaatcaccg ggggaaaata tgacccggag
     2401 tgtgaggtcg gattgcacta tcagattcac cgcggaattg gcattcatca tgcgcgtgcc
     2461 attgaagcca agcagaaact ccgcgtcaat gtcttcgtgg gtggggcacc tgccatgacg
     2521 gttgccgccg tcatgccact tcccgaaggg atgagcgagc tattcttcgc tgggcttttg
     2581 ggcggacatc gtattcccat gattcgccgg aaaggggaac tcccgatcta cgccgaggcc
     2641 gatttcgttc tcaccggcta tattgaaccc aagaaactac tgcccgaggg acctttcggt
     2701 gaccacctgg ggtattacag ccttgtgcac gattttccgg tgatgcatgt agagcacgtg
     2761 taccatcgcc caaatcccat ttggccgttt acggtcgtgg ggcgaccacc ccaagaggat
     2821 tcgatgtttg cccagttgat tcacgagctt gtcggtcccg ttattcctaa agccatccca
     2881 ggtgtgcggg ccgtccatgc cgtcgacgcc gccggggtcc atcctctctt gttggccatc
     2941 ggtagtgagc gatatacgcc atacgacgaa cgaaaacggc cacaagaatt gttgacattg
     3001 gccaatgccc tgttaggaca cggccagttg tcattgacca agtacttgtt tattgtcgcc
     3061 ggcgaggata accccgatct ggacgtccac gatgtggatg acttcttccg acatgtttta
     3121 gaacgggtgg attggcggcg ggatgtgcat ttccaaacct gcacgacggt ggatacgctg
     3181 gattacacag gcggggcatt gaaccagggc tcaaaagtga ccattgccgc agtgggaaaa
     3241 ccgcgacgca cgcttcccgt cgcgctcgat tcacgaatca agattcccga agacctgggg
     3301 ttttccgatc ctcgggtgat gttgcccggg atcctggtga ttcaaggtcc tccctaccgc
     3361 cgcaacgaaa aaggggaaga cgaatcgatc cggcagtttt gtgcacggta ttctcggcac
     3421 gatgcggtca atcttttccc tctcattgtg atcgtggatg atagcgagtt tgccgcccga
     3481 acgctgaaca actttctctg gacaaccttc acccgaagcg atcccgccgg ggatattcac
     3541 ggcattgaag aattcgtatc gaaaaaacat tggggatgcc acggttcgtt ggtcattgac
     3601 gcgcgggcca agccggaaca cccgccgcct ctggtcgagg atcccgaagt tgtccgccgt
     3661 gtcgaggcgc tggccgccc
//
LOCUS       DAOPTR010000303         3668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_20188_length_3668_cov_4.229172, whole genome shotgun sequence.
ACCESSION   DAOPTR010000303 DAOPTR010000000
VERSION     DAOPTR010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20188_length_3668_cov_4.229172"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="PKI05_06890"
     CDS             complement(<1..1319)
                     /locus_tag="PKI05_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51966.1"
                     /translation="MSESQPQSPDSFFGPLLAKITCPHCWHRFATPDTLWISEHPDLI
                     GDRLLGPYIPQRFLPNRFTPQGKAIDSRGMETSRLACPNCHLEIPRGAYHLRSLFLSV
                     LGAPASGKSYFLTSMVWRLRRVLPEEFAVTFTDLDAIHNQRLQEYENALFLNEDEDHP
                     VELPKTETTGDLYNTVLFEDSPIQYIKPFLFAVQPMGRHPRVKARSQISRMLVIYDNA
                     GESFLPGGDSAQNPVTRHLALSAAWFFVFDPTQDLRFRKEVSQFSNDPQVRPDGPRGR
                     SVTQRQEAVLHEAWSRVLQHRDSQSSTRAIPLIVVCAKADCWGPLIPGWPLPSPYRRS
                     TQGVAGVDMDVLADVSNKVRELLNRLSPEIVTAAESLTSDVIYLPVSATGCSPETDEN
                     GQIVGFLPKKLNPIWVEVPLIYFMVRWLPTLVFRLRGVYGRRALQPD"
     gene            complement(1517..2983)
                     /locus_tag="PKI05_06895"
     CDS             complement(1517..2983)
                     /locus_tag="PKI05_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOA51967.1"
                     /translation="MKRVTIDPITRLEGHGKIEIFLNDQGEVANAYLQIPELRGFERF
                     CVGRPVEEMPNLTARICGVCPEAHHMAATKALDAVFHVDPPATAKKLRELFYSIFFAT
                     DHTTHFYALGGPDFVVGPDAPKAERNILGVINKVGLEIGGQVIKMRHVGHNLIQLMGG
                     RRVHPNWGLPGGVSRGITEEERREIEAAGREAVEFAKFSLQLFDKIVLKNSEYVALIT
                     GDVYVQKTYYMGTVDANNHVNFYDGMIRVVGPDGKEHAKYHPKDYRDYVAERVEPWSY
                     LKFPYLKKVGWKGFVDGPDSGVYQATPLSRLNASDGMATPLAQKEWQRFYEVLGGRPV
                     HHTLATHWARLIELLYATERWVELASDPSITGKDFRVLPKEKPTEGVGCVEAPRGTLT
                     HHYVTDERGLVVKANLIVGTTNNNASICMAVRRAAQKLIKGGQIREGLLNRVEMAFRS
                     YDPCFGCATHSLPGQMPLEVVIRNSSGEVVERLSQYLD"
     gene            complement(3042..>3668)
                     /locus_tag="PKI05_06900"
     CDS             complement(3042..>3668)
                     /locus_tag="PKI05_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-nonreducing hydrogenase"
                     /protein_id="HOA51968.1"
                     /translation="PSTENPQHIVPQHETEVPEGKLYLPVFYDTVFTLDQVVPVDYYL
                     PGCPPEAERIWDAIVAILEGKLPPKGSVIGANTTVCDECPRKRTEKKIKKFYRTWEII
                     PDEETCLLEQGIICCGVATRAGCGARCPRVNSPCIGCYGPNTDVIDYGARLMAALASV
                     IDSNDPQEIEKIIQDGIPDPVGTFYRFSLAHSLLRRKRKLVAAAMGGN"
BASE COUNT          842 a         1058 c         1000 g          768 t
ORIGIN      
        1 tcgtcaggct gcaaagcccg acgtccgtaa acgcctcgca aacgaaatac aagcgtgggc
       61 agccagcgaa ccataaagta gatcagcgga acttccaccc agatgggatt gagcttcttg
      121 ggcaaaaacc ccacgatctg gccgttctca tccgtttcgg gtgaacaccc tgtggcactg
      181 acgggcagat aaatgacatc ggatgtcagc gactccgcgg cggtgacaat ttccggtgaa
      241 agacggttaa gaagttcccg caccttattg gacacatcag ccagtacatc catatcgaca
      301 ccggccaccc cctgggtgct ccgtcggtac ggcgaaggca acggccagcc gggaataagt
      361 ggtccccaac agtcggcctt ggcacagacg acgatcaagg ggatggcgcg ggtactggat
      421 tggctatccc tatgttgcag gacacggctc cacgcttcgt ggaggaccgc ctcttgtcgc
      481 tgagtcacac tgcgtccccg cggcccatcc ggccgaacct ggggatcatt tgaaaactgg
      541 ctcacttcct tgcgaaaacg taaatcctgg gtgggatcaa agacaaagaa ccacgcggcg
      601 gaaagggcaa gatgtcgcgt cacggggttt tgggcggagt ccccgccggg aaggaaactc
      661 tcgcccgcat tgtcgtaaat gaccagcatg cggctgatct gcgaccgggc cttcactcgc
      721 ggatgccttc ccatgggttg cacggcaaag agaaacggct tgatatattg gatcggcgaa
      781 tcctcaaaga ggaccgtatt gtacaagtcg cccgttgttt ccgtcttggg cagttctacc
      841 gggtgatctt catcctcgtt caaaaacagg gcgttttcat attcctgaag ccgttgatta
      901 tgaatggcat ccagatcggt aaaggtcacg gcgaactctt caggcaacac tctccgcagg
      961 cgccacacca tactggtgag gaaataggat ttgcccgaag ctggtgcccc caagaccgac
     1021 aaaaacagcg accgcaggtg atacgcccct cgggggattt ccaaatggca gttgggacaa
     1081 gcaagtcgcg aggtctccat cccccggctg tcaatggcct ttccctgggg tgtgaaacga
     1141 ttgggaagaa accgttgtgg gatgtaaggg ccaagcaggc gatccccaat caaatctgga
     1201 tgttcggaaa tccataacgt gtcaggagtg gcaaagcggt gccagcaatg cggacacgtg
     1261 atctttgcga ggagcggccc aaaaaaggag tcaggacttt gcggttgcga ttcggacatg
     1321 taccacctcc gggaatgccc ctcttattag tgtccgttcc ccatcgaggg cggtcaatga
     1381 ccaaatctcc gggaccgtat caccatttcc agatgtgccc cccaaaaaag aaaaaaagcg
     1441 aggagatcat ttatcccctc gcaggttaaa ggacactccg tcatggagcg gtgggatgat
     1501 gccgggtgat accccgtcag tccagatact ggcttagacg ctccaccacc tcgccggaag
     1561 aattgcggat gacgacttcc agcggcattt ggccgggcag gctatgtgtt gcacagccga
     1621 agcatgggtc ataggaacgg aaggccatct ccacgcggtt gagcaaacct tcgcggatct
     1681 ggccaccctt gatgagcttt tgggccgccc ggcgcactgc catgcagatg gaggcattgt
     1741 tgttggtcgt cccgacaatc aggtttgctt tgaccaccag gccacgctca tcggtcacat
     1801 agtgatgggt aagggtaccg cgaggggcct ccacacaccc gacgccttcg gtgggctttt
     1861 cttttggcag tacgcggaaa tccttccccg tgatgcttgg atcggaggcc aattccaccc
     1921 aacgctcggt ggcgtacagc aattcgatca agcgggccca gtgcgtggcc agcgtatgat
     1981 gcaccggacg accaccaagc acttcgtaaa accgctgcca ttccttctga gccagaggag
     2041 tggccatgcc atcggaagcg ttaagccgtg acagcggtgt ggcctgatag acaccggaat
     2101 ctggaccatc gacgaagccc ttccaaccga ccttcttgag gtacgggaac ttcaaatagc
     2161 tccaaggctc gacgcgctcg gccacatagt ctcgataatc tttgggatga tacttggcgt
     2221 gttccttccc gtccggcccg accacgcgaa tcatgccgtc ataaaaattc acgtggttat
     2281 tggcatctac ggtgcccatg tagtaggtct tttgaacata aacatcgccc gtgatgaggg
     2341 cgacgtactc actatttttc agcacgatct tatcgaagag ctggaggctg aacttggcga
     2401 actcgacggc ttcacgtccg gcggcctcga tctcacgtcg ttcctcttct gtgatccctc
     2461 ggctgacacc accgggaagg ccccagttgg gatgaacgcg gcgaccgccc atcaactgga
     2521 tgaggttatg gccaacatgc cgcatcttga tgacctgacc accgatttcc aagcctacct
     2581 tgttaatcac gccaaggatg tttcgttcgg ccttgggggc atctggccca acaacgaaat
     2641 cgggaccacc caaagcgtaa aagtgggtcg tgtggtcggt ggcgaaaaag atgctgtaaa
     2701 acaactcgcg aagtttcttg gccgtggccg gaggatcaac gtggaacacc gcatccaggg
     2761 ccttggtggc ggccatgtgg tgggcttccg gacagacgcc gcaaatccgg gccgtcaggt
     2821 tgggcatctc ttccactggc cgacctacgc aaaaccgttc aaatccccgc aattccggaa
     2881 tctgcaaata cgcgttggcc acctcgcctt ggtcattcag aaagatctcg atctttccgt
     2941 gaccttccaa ccgggtgatg ggatcaatcg tcacgcgttt catagttccc tcaagtcgcc
     3001 ttcaataagg atcgtactga gtgttgttga ccaagttaac cttagttacc ccccatggcg
     3061 gcggcgacca gctttcgttt acgccgcaac aaactatggg ccaaactaaa gcggtaaaaa
     3121 gtccccacgg gatcagggat accatcttga atgatcttct cgatctcctg ggggtcgtta
     3181 gaatcaataa ccgatgccaa ggccgccatc agacgtgcgc cgtaatcaat gacatccgtg
     3241 ttcggtccat agcacccaat gcacggcgaa ttaacacgtg gacagcgtgc cccgcaaccc
     3301 gcccgcgtgg caacaccaca acagataatc ccctgctcca agaggcacgt ttcctcgtcc
     3361 ggaatgattt cccacgttcg gtagaacttc ttgatcttct tttccgtccg cttccgggga
     3421 cactcatcgc acacggtggt gttggcccca atcacggacc ccttcggcgg gagctttcct
     3481 tccaagatgg ccacaatcgc gtcccaaatc cgttccgcct ctggtgggca accggggagg
     3541 tagtaatcca ccggcacaac ctgatccaac gtaaagactg tgtcatagaa gaccggcaaa
     3601 tagagcttcc cttccgggac ttccgtctca tgctggggaa caatgtgctg cggattctcg
     3661 gtggaagg
//
LOCUS       DAOPTR010000304         3664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_20218_length_3664_cov_2.743973, whole genome shotgun sequence.
ACCESSION   DAOPTR010000304 DAOPTR010000000
VERSION     DAOPTR010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20218_length_3664_cov_2.743973"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..420)
                     /gene="hypA"
                     /locus_tag="PKI05_06905"
     CDS             complement(46..420)
                     /gene="hypA"
                     /locus_tag="PKI05_06905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOA51969.1"
                     /translation="MHEVALVESLLESVHEQLREAGIVGRVRRLDVIIGPLSGVVPEA
                     FRFAFKVLSPESLGPGCELVIHTRPARCQCQSCGAVSEIAELTYVCPVCGSHDIRLEG
                     GRELMLQSIEVDENDPCGDPPA"
     gene            complement(456..899)
                     /locus_tag="PKI05_06910"
     CDS             complement(456..899)
                     /locus_tag="PKI05_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOA51970.1"
                     /translation="MVSPEMLRRYSYFAKVSEETLKEIAMLAEEKSYPAGMRLFYVGD
                     PADHLNIIVEGEVDIQYELGNGEKRTVDTLVPGDILVWSALVEPYRTTAIGTTTKPTK
                     VIAIKAKPLRELCDKDSMLGYQLMREIAKLLASRLESARVQLAAT"
     gene            complement(1001..1528)
                     /locus_tag="PKI05_06915"
     CDS             complement(1001..1528)
                     /locus_tag="PKI05_06915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOA51971.1"
                     /translation="MMKRTLVLGLGNDILTDDAVGLRVARELKSILAGRPEITVDEDC
                     WGGLRLMERLAVFDRAIIIDAIQSGGQPGTVRILSPKDIPTQHSASAHDVNLPTALAF
                     GRKMGVHLPEDHEIVLVAIEALDILTFGERCTPAVEAAIPQAVRLVLDLLAGWDNSLP
                     DRNSGLGFGQSTREL"
     gene            complement(1564..2694)
                     /locus_tag="PKI05_06920"
     CDS             complement(1564..2694)
                     /locus_tag="PKI05_06920"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOA51972.1"
                     /translation="MTTTNTTRTLDGVGLTRTWGGAVGRERLQVLCDTYNRLLNQRRR
                     LNRTEHLWLIRELGRGGQGVVYLSERRGADGFTLPLALKFFAPDPYPNDAAYEEAMAR
                     IGWVSGRVSRIAHDNLLDVDNWLDCDGIRVMVMEWVDGYDLARLLTKRMYQLLEQRVK
                     QIRWQYINEVIVTAGPVQPRLKPGVAVAIVRDCLAGLAALHRGRIVHGDIKPSNIMLK
                     RTGTAKLVDVGSAVDLDNPPPMRGCTPLYAAPEVLESDGAEISPRSDLASLGYVLIEM
                     LSGQPPFPANLPRAQLLEAKRLLPQRLHTILPPECVASEALMNFCKMLIAPDPARRFS
                     NAESADLDNNGAAAFLRQLVKGDLASEYATEIRHWLSDLHGL"
     gene            3144..>3664
                     /locus_tag="PKI05_06925"
     CDS             3144..>3664
                     /locus_tag="PKI05_06925"
                     /inference="COORDINATES: protein motif:HMM:NF022135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HOA51973.1"
                     /translation="MLKMGQAERWASRTGIFLRIARSGGEAVIRWSILAIFLAIAAPV
                     QAHFLVLLPSQDVVDTPEQATLVFDIQFTHPMEQGPVMAMGTPSRFFVSHGGQQIDLI
                     TLLQPRSLQGQPAFVARYRLNSPGDYVFAIEPAPYWEKMEKKWIVHYTKVVVNFMGDE
                     GDWAKPVGLPVEI"
BASE COUNT          818 a         1099 c          953 g          794 t
ORIGIN      
        1 ccaaccctct cccagaggga gagggggact ttttggggat aggctctaag ccggtgggtc
       61 accgcaaggg tcattttcat ccacttcaat ggattggagc atcagctcac gcccaccttc
      121 caagcgtata tcatgactgc cgcagaccgg gcaaacgtag gtcagctccg caatctccga
      181 gaccgcccca catgattggc actggcatcg agccggtcga gtgtggatta ccaactcgca
      241 gccgggtccg agactctcgg gcgaaagaac tttaaaggca aagcgaaagg cctccggcac
      301 aacacccgac aggggcccaa taataacatc taaccgtcgg actcgcccga caatccccgc
      361 ctcccgaagt tgctcgtgca cagactccag gagggactcc accaacgcga cttcatgcat
      421 gtcggtttgc gaattcttac actaagagcc acgcttcagg tggcggccag ttggacccgc
      481 gcggactcaa gacgactggc caagagtttc gcgatttctc gcatcaactg gtaaccgagc
      541 atcgagtcct tgtcgcacaa ctcccgtaag ggcttggctt tgatggcgat gaccttcgtc
      601 ggttttgttg tcgtcccaat cgcggtcgtt cggtacggtt caaccaacgc tgaccagacg
      661 aggatgtctc caggcacgag cgtatcgacg gttcgtttct ccccgttacc cagctcgtat
      721 tggatgtcca cttctccctc aacaataatg ttgagatggt ccgcgggatc accgacgtaa
      781 aatagccgca tccccgcagg atacgacttt tcttccgcca gcatggcgat ttccttgagc
      841 gtctcctcgc taactttggc gaaatacgaa taacgacgca acatttcggg cgaaaccatg
      901 gtcaaactcc tcccagtctt ggtgaccgtt agcagggcct atcgcaccca tgatcccaac
      961 agcctgtcgc tttagggcgc tgatcctggc caagcaacag tcacagttcc cgcgtgctct
     1021 gtccaaaccc gagacccgaa ttgcggtcag gcaatgagtt gtcccacccg gcgaggagat
     1081 ccaacacaag ccgcaccgcc tggggaatgg ctgcctccac cgcgggagtg caccgttccc
     1141 caaaagtcag tatatccagt gcttcaatgg cgacaaggac aatctcatga tcttctggga
     1201 ggtgtacccc catttttcgc ccaaaggcca aggctgtcgg caagtttacg tcgtgggcag
     1261 aagcactgtg ttgggtaggg atgtcttttg gcgagagaat tcggactgtc cccggctgcc
     1321 cgcctgattg aatcgcatcg atgataatcg cccgatcaaa cacagccagc cgctccatca
     1381 atcgcaggcc gccccagcaa tcttcatcca cggtaatctc aggacgcccg gccaagatcg
     1441 atttcaattc cctcgccaca cgcagcccca cggcgtcatc cgtgaggata tcgtttccca
     1501 atccgaggac caaggtgcgt ttcatcattg ttaggcgaag atcacggcaa gggggcgacc
     1561 agattacagg ccatgcaaat cagaaagcca atgccgaatt tccgtggcgt attcggaagc
     1621 caggtcaccc ttcacaagtt gtcgtaagaa ggccgctgcg ccattattgt ccaaatcggc
     1681 cgactcggca ttggagaacc gccgggccgg gtccggtgcg atcaacatct tgcagaagtt
     1741 catcagggcc tcactggcca cacactctgg cggtaaaatg gtgtgtaatc tctgcggtaa
     1801 aagacgcttc gcctccaaaa gttgagcccg tggcaaattg gcaggaaacg gcggctggcc
     1861 cgacaacatc tcgatcaaga cgtaacccaa acttgccaaa tccgagcggg gtgatatctc
     1921 ggccccatcg ctctccaaca cctcaggggc cgcatataag ggcgtgcatc cgcgcatcgg
     1981 tggcggattg tctaaatcca cagccgaacc cacatcaacc aatttggccg tcccggttcg
     2041 cttgagcatg atgttcgaag gcttaatgtc accatgcacg attcgccctc gatggagcgc
     2101 cgcgagcccc gccaaacagt ccctgacgat ggcgacggcc acgccgggtt tgagccgtgg
     2161 ctgcaccggg cccgccgtaa cgatcacctc gttgatatat tgccagcgga tctgtttcac
     2221 acgttgttcc agaagttggt acatccgttt ggtcaagagg cgggctaaat cgtagccatc
     2281 gacccattcc atgaccatca cgcggatgcc gtcgcagtcc aaccagttat ccacatcgag
     2341 gagattatca tgagcgatcc gagaaacacg gccagagacc caaccgatcc tggccatggc
     2401 ctcctcgtac gcggcatcat tgggataggg atcaggggca aagaacttta acgcgagggg
     2461 caacgtaaat ccgtccgccc cccgtcgctc gcttagatag acaacccctt gtcccccgcg
     2521 gccaagttcg cggatcaacc atagatgctc ggtgcgattg agccgtcgcc gttgattgag
     2581 caggcggttg tatgtgtcac aaaggacctg gagtcgttct cgaccaactg caccgcccca
     2641 agtccgtgtc aatccaaccc cgtcaagggt tctcgtcgta ttagtcgtgg tcatgtatat
     2701 caaatctcgt gaaaaatgat cgaatcagac acctccttgt gttatcacaa tcatacgaaa
     2761 aaccttgcct catcgtaaag attgcaatgc acgtttcgcc agaaggactg gccctgctcc
     2821 acctatgtgt gtgacatcag tttgactact ccggtgttac ggccggctaa tggtttctta
     2881 gagcgagaca cgcggaaaag gcgcttcggt tcgctctttg tcccaacgct gaccagttga
     2941 tggccactgc gcgaaagcat cccgctgcct tcgcggtcgc cacaaaagcg tggcccgtcc
     3001 agctccagat gatgcctcgt tctcaatccc ctttcaggtc atggacttcc cttttctttg
     3061 ggtgctacaa taacagaatt ggtaacactg accttgcgtc cgttttcata cctttgccat
     3121 tgtgtggtgg ggcgtgcgtt tccatgctca agatgggtca agccgaacga tgggcatcac
     3181 ggaccggcat ctttttgagg atcgcccggt ctggagggga agccgtcatc cgatggagca
     3241 tcctggccat cttcctggca atcgccgcac cggtgcaggc ccattttctt gtcctgctcc
     3301 cctcccagga tgtggtggat acgcccgagc aggccaccct cgtgtttgac atccagttta
     3361 ctcatcccat ggaacaagga cccgtgatgg ccatgggaac gccttcccgt ttttttgtaa
     3421 gccatggggg tcagcagatc gatctgatca ccctattgca gccccggtcg cttcaaggcc
     3481 agcctgcctt tgtcgcccgc tatcgactga acagtcccgg cgattacgtg tttgccatcg
     3541 agccggcacc ctattgggaa aagatggaga agaaatggat tgtccactac accaaagtcg
     3601 tggtcaactt tatgggagac gaaggagatt gggcaaaacc cgtgggtttg ccggtcgaaa
     3661 ttca
//
LOCUS       DAOPTR010000305         3659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_20256_length_3659_cov_4.120699, whole genome shotgun sequence.
ACCESSION   DAOPTR010000305 DAOPTR010000000
VERSION     DAOPTR010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20256_length_3659_cov_4.120699"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="PKI05_06930"
     CDS             complement(<1..800)
                     /locus_tag="PKI05_06930"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOA51974.1"
                     /translation="MAKHIFVTGGVVSSLGKGLTSASIGMLLEHRGLSVRMQKMDPYI
                     NVDPGTMSPYQHGEVYVLDDGSETDLDLGHYERFTNSPLTKDSNYTTGKIYLSVITKE
                     RRGEYLGQTVQVIPHITNEIKRAITALGGPGVDVVITEIGGTVGDIESLPFLEAIRQL
                     SLDLGRENCLYIHLTLVPYLRAAGEIKTKPTQHSVGQLRQIGIQPDILICRTEKPLGR
                     DARAKIALFCNVPVECVIEERDKDFSIYEVPLSLVENNLDELIVRKLGL"
     gene            complement(942..1700)
                     /gene="kdsB"
                     /locus_tag="PKI05_06935"
     CDS             complement(942..1700)
                     /gene="kdsB"
                     /locus_tag="PKI05_06935"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOA51975.1"
                     /translation="MKSWIVIPARLASTRLPRKMLLSETGKPLIQHTYEAASQARRPA
                     GICVACDDEEIARVVRSFGGDARLTSPHAQSGTDRVAEIARSMPDVEIFVNVQGDEPE
                     ISGEAVDLAVELLERHPQASVSTVAAPLRNREALHDPACVKVVCDKDGYALYFSRSVI
                     PYPREWSEEYLTASPPCFLQHMGLYAYRRSFLLSVSNLEVPYLERLEKLEQLRFLYHG
                     AKIVVGLVDSPTCGIDTPEDYRRFVERYRARYTR"
     gene            complement(1782..2807)
                     /locus_tag="PKI05_06940"
     CDS             complement(1782..2807)
                     /locus_tag="PKI05_06940"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOA51976.1"
                     /translation="MRPTSKGFRWFLYSAGVLLLLLQTEPLLAQKWAEAMFDHTSHNF
                     GMVARGAKVEHVFPLKNIYVEDVHIASVRTSCMCTQPTITKETLKTFETGAIVATVDT
                     RGFLGRKDATITVTFDKPYPAEVQLHTYVYIRSDVVFEPGAVLFNAIPQGTSAHRTVQ
                     LSYAGRNDWRIVRAVPSADFLEVEVNETGRGAGEVRYQLVVTLKPQTPPGYLQEHIEL
                     FTNDTVADAQKIVLAVEGSIVPQVSIQPSQLALGSVTPGQVVTRNLVIQARKPFRVVS
                     IKGPDERFSFVLPETTKSVQVIPVIFKAGDRAEPIKGEIQIATDLAGFETLRVSVFGT
                     FAGTDAQ"
     gene            complement(3136..>3659)
                     /locus_tag="PKI05_06945"
     CDS             complement(3136..>3659)
                     /locus_tag="PKI05_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOA51977.1"
                     /translation="TTFANFDAVIQLAADVRRAESLGDVKLVLITNASMFHREVVQRG
                     LAILDENNGEIWAKLDAGTPEFFAKVARTRIPFERILENITQAAQIRPIVIQSLFMRW
                     LGSPPEEAEIEAYCMRLNEITRRGGHIKGIQIYTVARPPAESFISPLNKDEIDAIASR
                     VAEKTGLAVATFL"
BASE COUNT          833 a         1047 c          985 g          794 t
ORIGIN      
        1 aggccaagct tccgcacgat cagctcatcc agattgtttt ccacaaggct aagggggacc
       61 tcataaatag agaaatcctt gtcgcgttct tcaatgacgc actccacggg gacattgcag
      121 aagagggcga tcttagcacg ggcatcccgg cccaagggct tttcggtccg acaaatgagg
      181 atgtccggct ggataccgat ctggcgaagc tgtccgaccg aatgttgcgt gggcttggtc
      241 ttaatctctc cggctgcgcg aagatagggg accaaggtca aatggatata caggcaattc
      301 tcgcggccta agtcgaggga taactggcgg atggcttcca aaaaggggag gctttcaatg
      361 tcgcccacgg tcccgccaat ctctgtgatg acgacatcga cgcccggtcc acccagggcc
      421 gtgatggccc gcttaatctc gtttgtaatg tgcggaatga cttgaaccgt ctgccccaga
      481 tactcgccac gacgttcttt cgtgatgaca gaaagataaa tcttgcccgt cgtgtagttg
      541 ctatccttcg tcagagggct gttggtaaag cgctcgtaat ggccgaggtc gagatcggtc
      601 tcgctgccgt catcgagaac gtaaacctcc ccgtgctgat agggactcat tgtccctgga
      661 tccacgttga tgtacgggtc catcttctgc atccgcacgg aaagtccccg atgctccaaa
      721 agcatcccga tcgatgcact cgtcagccct ttgcccaagg aactgaccac accaccggtg
      781 acaaaaatgt gcttggccat ttgtgcgaat ctcgctcata aaacaaaaag aaccgcatcc
      841 tcaggttttt tgatgcggcc gaacatcggc aaggatcaga cggtgcgtcc gcagggcctg
      901 ccatgacaac cgcgcagacg ccacctagag actcaacctc tctaccgagt gtaccgcgcc
      961 cggtatcgct cgacaaaccg tcggtagtct tcgggggtat ctatcccgca ggtgggggaa
     1021 tcaaccaggc ccaccacgat cttggccccg tggtacaaga agcggagctg ttccagtttt
     1081 tcgagacgct ccaaataggg aacttcgaga ttcgagacac tgaggaggaa cgaccgtcga
     1141 taggcgtaca atcccatgtg ctgcaaaaaa cacggcggcg aggcggtgag atattcctcg
     1201 ctccattccc tcggatacgg aatgacacta cggctgaaat atagggcgta gccgtccttg
     1261 tcgcacacaa cctttacgca ggccggatca tgcaatgctt cccgattccg caaaggtgcg
     1321 gccactgtgg aaacgctggc ctggggatgc ctttccagga gttccaccgc cagatcaacc
     1381 gcctcgccgg aaatctcggg ttcatcgcct tgaacgttga caaatatctc cacgtcgggc
     1441 atagaccgag caatctctgc cacgcggtcc gtcccgcttt gggcgtgggg acttgtcaag
     1501 cgggcgtcgc caccgaagga gcgaacgacg cgggcaatct cctcatcatc gcaagccaca
     1561 cagattccgg ccggtcgtcg ggcttgggag gctgcctcat acgtgtgctg aatgagcggt
     1621 ttgcccgtct cggaaagaag catctttcgc ggcaaacgcg tggaagccaa acgcgcaggg
     1681 atgacaatcc aacttttcat tgacattcac ctggtgctct acgaacgatc attagtgcag
     1741 gaagagtgag cgagtcccaa cgcaccgaac gactctatct gtcattgcgc atcggtgccg
     1801 gcaaacgtcc caaaaacgga gacacgaagc gtctcgaaac cggctaaatc ggtcgcgatc
     1861 tggatctctc ccttgatggg ttccgcacgg tcacctgcct taaaaatgac cggaatgact
     1921 tgcacgctct tggtcgtttc gggcaggaca aacgaaaagc gttcatcggg ccccttaatg
     1981 gacacaaccc gaaacggctt gcgggcttga atcaccagat tccgggtgac aacctgaccg
     2041 ggcgtgacgc tacccaatgc gagttgcgaa ggctgaatcg agacctgggg cacgatggaa
     2101 ccttccacgg ccaggacaat cttttgggca tcggccacag tatcgttcgt aaatagctcg
     2161 atgtgctctt ggagatagcc cggcggcgtc tgcggcttaa gcgtcactac aagctgatag
     2221 cggacctctc cggccccacg acccgtttca ttgacttcta cctccagaaa atccgcactt
     2281 ggcactgccc gcacgattcg ccagtcgttt cgccctgcgt agctgagctg gaccgtcctg
     2341 tgcgcgctcg tgccttgggg tatcgcattg aagagcacgg ccccaggttc aaagaccacg
     2401 tcgctgcgga tgtaaacgta cgtatgcaac tggacttccg ccggataagg cttatcaaaa
     2461 gtcaccgtga tggtggcatc cttgcgtccc agaaaccctc gcgtgtccac cgtggcgaca
     2521 atggcccccg tttcaaaggt cttaagtgtc tccttagtaa ttgtgggttg cgtgcacatg
     2581 caggatgtcc gaacggaggc gatgtggaca tcctcgacat aaatgttttt caaagggaaa
     2641 acatgctcaa ctttggcacc ccgggcgacc atcccgaagt tgtggctcgt atgatcgaac
     2701 atcgcctccg cccacttctg ggccagtaac ggttctgttt gcagaagtaa tagcaataca
     2761 ccagccgagt aaagaaacca ccgaaatcct ttcgatgtgg gtctcatcgt gatgaaactc
     2821 cctaaacacg ccggatgcat caccatccct gctcgttgtt agcggtgtgc tgccgcaagg
     2881 agggctcgta gatcgttcgg accggaatct cagaaaactg accggttggc gcaagttggg
     2941 ttatctttaa cggcaaaaga ataacgtctt ttactacgct tggcaatgca gattggcaat
     3001 ctttgatcga ccgcagccgt cttcgttagc ccccgatgca ggtcggcgcc tttctcacat
     3061 tgcccaactt actcatattg cccaggcctc gcatcgtttt gcggcctagg ctatatgaac
     3121 cgacactagc ggggtttaaa ggaacgttgc cacagcgagg cccgtcttct cagcaacgcg
     3181 gctggcaatc gcatcgattt catccttatt cagtggggag ataaacgatt ccgccggggg
     3241 acgtgccacg gtgtagattt gaatcccctt aatgtgcccg cctcgccgag taatttcgtt
     3301 taaacgcatg caataggcct cgatttccgc ttcttctggc ggggagccaa gccacctcat
     3361 gaacagcgat tggatgacga tcggccgaat ctgggcagcc tgggtgatat tttcgagaat
     3421 tcgttcaaag ggaattcgcg tccgcgcaac cttggcaaaa aactccggcg tgccggcatc
     3481 caacttggcc cagatctcgc cgttgttttc gtcgagaatg gccaaacccc gttggaccac
     3541 ctctcggtga aacatggagg cattcgtgat gaggacaagt ttcacatcgc ccagtgactc
     3601 ggcacgacgg acgtccgccg caagctgaat cacggcatcg aaattggcga acgtcgttg
//
LOCUS       DAOPTR010000306         3659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_20258_length_3659_cov_3.336293, whole genome shotgun sequence.
ACCESSION   DAOPTR010000306 DAOPTR010000000
VERSION     DAOPTR010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20258_length_3659_cov_3.336293"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PKI05_06950"
     CDS             complement(<1..815)
                     /locus_tag="PKI05_06950"
                     /inference="COORDINATES: protein motif:HMM:NF016240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HOA51978.1"
                     /translation="MMEENHSIEQPTHISQEAERRWRPLPPIERRILGVLVEKAKTTP
                     SAYPMTLNAIVTGCNQKSNRDPVMELNEEVVDRALENLRQMGAVGRVQGLGRVDKYRH
                     YLYEWLNVEKEELSIMAELLLRGPQTEGELRAHVSRMDPIPDLPTLRQHLDRLTKKGL
                     IVHLTPPGRGHTVAHNLYPEPELARLKQRYAALAVTDTAFADSEPPSSSLSAPAATAT
                     MARAGGDTVGRSAETSPPPQAHEIDALRSEIAALKETLTRVVGDVERIKRELGL"
     gene            complement(838..3096)
                     /locus_tag="PKI05_06955"
     CDS             complement(838..3096)
                     /locus_tag="PKI05_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOA51979.1"
                     /translation="MHEDLLGKLTPAQREAVTHVEGPLLILAGPGSGKTRVVTFRIAY
                     LLRLGIPDKNILGLTFTNKAADEMRTRVEALVPGARVWLGTFHRFAAQTLRQYAQFVG
                     LEPNFTIYDKENSLRALKAVINDANIDLGSYSVESVAHAISTAKSALILPTDYLPREG
                     SPLGELVRRIYPLYQQRLIQSNAVDFDDLLMHLAILLRQHPEIRKRLDDRYRFVMVDE
                     YQDTNLVQYAIARAISVDHPNLAVTGDPDQSIYGWRGANLNNILEFEKDFPNVRVVRL
                     EQNYRSTKRILAVAARLIAHNVKRKEKDLFTENAEGRPVRLMTYANQEDEARDIARQI
                     AQAVATGQRRYRDFALFYRINALSRSFEIAFREFGIPYQIVNGVEFFQRKEIKDIIAY
                     LQLINNPRDDVAFLRIINTPPRSIGETSVKRLRQFAAEQRLSLLEAARLADRIGEFST
                     RTVGAFRGFVNMMDRLAAAADGPVEELLGLVMTESGYRAMLEKSSREEDLERLANIEE
                     LLTAARQFDERHQEEANLERFLEETALVSDTDKMDDRSDRVTLMTLHASKGLEFPVVY
                     LVAVEQGILPHERSRNNDDELEEERRLMFVGITRAKEELQISLAEYRDFRGQRQLTIP
                     SQFLMELPRSELEIVQSPRAVGRVSTFETATGSWARVVSSRFENGNRSQSGSSTSAIP
                     PRTPSTSASRRGPLTPDDFVLDMRVVHPRYGVGRIVALSGSGESRKATVDFGGSIGRM
                     KFVLSQSPLKPK"
     gene            complement(3191..>3659)
                     /locus_tag="PKI05_06960"
     CDS             complement(3191..>3659)
                     /locus_tag="PKI05_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOA51980.1"
                     /translation="FVEACPDDLPSMLSYKKFAAEKSLLNTPPVFAVYMVKLVTDWLI
                     REFGDLKRVADYNEEKANLIYDVIDRCGGFYQVHAAPGSRSTMNIAFRLPTQELEKEF
                     LKQAEARNLYNLQGHRSVGGCRASIYNAMPREGVVALRDFMLEFFEENRHRLA"
BASE COUNT          738 a         1044 c         1065 g          812 t
ORIGIN      
        1 agccccaatt cgcgtttgat tcgctctacg tctcccacca cacgggttaa cgtctccttg
       61 agggctgcga tctcactccg gagggcatcg atctcatgcg cctgcggggg cggagacgtt
      121 tccgcggacc ggcccactgt gtcgccacct gctcgcgcca tcgtcgccgt ggcggccgga
      181 gccgacaacg aagatgacgg cggctcagaa tcggcaaagg cagtgtccgt cacggccagt
      241 gctgcgtacc gctgcttcag ccgagccaac tcgggctccg gatagagatt gtgggctacg
      301 gtgtgacctc gcccgggcgg tgtcagatgc acaatgagcc cctttttcgt caggcgatcc
      361 aggtgctgtc tcaaggtggg taaatcagga atggggtcca tccgcgacac atgggcacgc
      421 agttcccctt ccgtttgtgg acctcttaga aggagctcgg ccatgatgga aagttcttct
      481 ttttccacat tgagccactc gtaaagataa tggcgatact tatcgacacg acccaggcct
      541 tggacccggc cgacagcccc catttgccgg aggttttcca gggcacgatc gacaacctcc
      601 tcgttcaact ccataacagg atcacgatta cttttttgat tgcagcctgt cacgatggca
      661 ttgagtgtca tgggataagc cgacggggtc gtcttggcct tttcgacgag cactccgagg
      721 atacggcgtt cgatgggagg cagggggcgc caccgccgtt ccgcctcttg cgatatgtga
      781 gtcggttgct cgatgctatg gttttcctcc atcatcagcg atatcctttt ctcacaatca
      841 cttgggtttc aagggacttt gcgagaggac aaatttcatc cgcccaatgc taccgccaaa
      901 atcgacggtg gccttccgcg attccccgga accactcagg gccacaattc ggccgacgcc
      961 gtaccgggga tgcacaacac gcatgtcgag tacgaagtca tccggcgtga gtggtccccg
     1021 gcgtgaagcc gaagtcgatg gggttcgcgg cgggatggcc gacgttgacg aaccactttg
     1081 cgaccggttg ccattctcaa accgtgagga gacgacgcgt gcccacgagc cggttgccgt
     1141 ctcaaaagtc gagactcgcc ccaccgcccg gggcgactgt acaatctcca attcggatcg
     1201 cggcagttcc atgaggaatt ggctgggaat ggtcaactgc cgttggccac ggaaatcgcg
     1261 gtactcggcc aggctgattt gcaactcttc cttggcccgc gtgattccca cgaacatgag
     1321 tcgtcgttct tcttccagtt cgtcatcgtt attgcggctg cgttcgtgcg gcagaattcc
     1381 ctgttccacg gcgacgagat agaccacggg aaactccagt cctttggagg catgcagggt
     1441 catcagcgtc acgcggtcgg atcggtcatc cattttatcg gtgtcgctga ccagggccgt
     1501 ttcttcgaga aagcgttcca aattggcctc ctcttggtgg cgttcgtcaa actggcgagc
     1561 ggcggtgagg agttcctcaa tgttggccag gcgctcaagg tcttcctcgc gtgaggattt
     1621 ctccaacatc gcgcggtagc ccgactccgt catgaccaga ccgagcaatt cttccactgg
     1681 gccatcggcg gctgctgcaa gacgatccat catattgaca aagccgcgaa aggcccccac
     1741 ggtccgggtg ctgaactcgc cgatccgatc tgccaggcga gcggcctcta gcaaggataa
     1801 ccgctgctcg gcggcaaatt gtcgtagccg cttgacgctc gtctcaccga tcgaccgcgg
     1861 cggtgtgttg atgatccgca aaaaggccac gtcatcccgc ggattattaa tcaattggag
     1921 ataggcaatg atgtccttga tctctttccg ctggaagaat tccacaccgt tgacaatctg
     1981 ataggggata ccgaattctc gaaaggcaat ttcaaagctc cgggacaggg cattgatccg
     2041 gtaaaacagc gcaaaatccc ggtaacggcg ctgacctgtt gccacagcct gggcaatctg
     2101 tcgggcgata tcgcgggctt catcctcctg attggcgtag gtcattaggc ggacggggcg
     2161 accttccgca ttttcggtga aaaggtcctt ttccttgcgt ttaacgttgt gagcgataag
     2221 ccgtgcggcg actgccaaga tccgctttgt gctgcgatag ttctgctcga gccgcaccac
     2281 gcgcacgttg gggaaatcct tctcgaattc gagaatgttg ttcagattgg caccgcgcca
     2341 gccgtaaatc gattggtcgg gatcgcccgt cacggctagg ttaggatgat cgacggagat
     2401 ggcccgcgca atggcatatt gcaccagatt tgtgtcctga tactcatcga ccatgacgaa
     2461 gcggtagcga tcgtcgagtc gcttacggat ttccggatgc tgacgaagga ggatggccag
     2521 atgcatcaac aggtcgtcaa agtccacggc attggactgg atgagccgct gttgataaag
     2581 cggatagatc cgtcgcacga gctcaccgag aggcgaacct tctcgcggca ggtagtccgt
     2641 tggtaagatg agggcactct tggccgtgct gatggcgtgg gccaccgatt ccacggaata
     2701 gcttcccagg tcaatattgg cgtcgttgat gacggccttg agagcgcgaa gactattttc
     2761 cttgtcgtaa atggtgaaat tcggttccag gccgacaaat tgggcatact gacgcagcgt
     2821 ttgcgcggcg aagcggtgaa acgtcccgag ccacacgcgg gcaccgggga caagggcctc
     2881 cacgcgggtg cgcatttcgt cggcggcttt gttggtaaag gtcagcccca ggatgttttt
     2941 gtcggggatt cccagccgca acaaataggc gatgcggaag gttacgacgc gcgttttgcc
     3001 gctgcctggt ccggccaaga tgaggagcgg cccctcgaca tgggttactg cctctcgctg
     3061 ggccggcgtc agtttgccca acaaatcttc gtgcatgtgg cgaccttcct tgagaagatt
     3121 ttatcctttc cggaatcgtg gacgatcccg gtgcccaatg ttttcccagc ccggaagctg
     3181 tcgggccaaa tcacgcgagc cgatgacgat tctcttcgaa gaattcgagc atgaaatcgc
     3241 ggagggcgac caccccttcc cgcggcatcg cgttgtaaat ggatgcccga caaccaccca
     3301 ccgaccggtg cccctgcaaa ttgtagaggt tgcgggcttc ggcctgtttg aggaattcct
     3361 tttccaattc ctgggtggga aggcggaaag cgatgttcat cgtggaacgg cttcccggtg
     3421 cggcgtggac ctgatagaac cccccgcagc ggtcaatgac gtcataaatc agattggcct
     3481 tttcctcgtt atagtctgcc acccgcttca gatccccaaa ttcgcggatt agccagtcgg
     3541 tcaccagctt gaccatatac acggcgaaaa ccggcggcgt attgagcagc gacttctccg
     3601 cggcgaattt cttgtaactt agcatcgagg ggaggtcatc cgggcaggct tcgacaaag
//
LOCUS       DAOPTR010000307         3659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_20261_length_3659_cov_2.950888, whole genome shotgun sequence.
ACCESSION   DAOPTR010000307 DAOPTR010000000
VERSION     DAOPTR010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20261_length_3659_cov_2.950888"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PKI05_06965"
     CDS             <1..290
                     /locus_tag="PKI05_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOA51981.1"
                     /translation="YMATILRNPGPIYSARYDKVPLRVVANSERKFPKQWIAENGHDV
                     TDDFIRYAKPLIGEDMVNLPMVDGRQRMTRLQPIYAEKKLPPYVPQADRGK"
     gene            373..1242
                     /locus_tag="PKI05_06970"
     CDS             373..1242
                     /locus_tag="PKI05_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOA51982.1"
                     /translation="MISLPRQKDFLVGIDSDGCAFDTMELKHKECFIPNIIYYYNLQG
                     VSKYAREVAEFVNLYSKSRGINRFPALIETLERLRRRPEVKARGVKVVVPESLRKWMQ
                     SEKKLGNPALEQAVQATNDPELKQALEWSRAVNRSVEWMVHGVPPFPFVRESLEKLSK
                     VADIIVVSATPEEALKREWAEHGLDKYVRAILGQESGTKKDFLGLSSQYPSGHSLMVG
                     DAPGDQKAAEANGALFFPINPGEEEKSWQEFFEKGIDRFLNGSFSGDYQKELLAHFDQ
                     CLPIDPPWVEEAD"
     gene            1308..2765
                     /gene="gnd"
                     /locus_tag="PKI05_06975"
     CDS             1308..2765
                     /gene="gnd"
                     /locus_tag="PKI05_06975"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HOA51983.1"
                     /translation="MNGQCDIAVIGLEVMGRNLALNIESRGYRVAVYNRTVEKMEKFI
                     EGPAKGKNIVGCRSLEELVAALAKPRKVMMMVKAGPAVDALIDQLIPLLEPGDILIDG
                     GNTHFADTERRTAYVESKGLLYIGTGVSGGEEGALKGPSLMPGGSEKAWPIVKPIFQA
                     IAAKVGPNKDIPCCDWVGPRGAGHYVKMVHNGIEYGDMQLIAEAYWLLKHGLGATNEE
                     LYDIFARWNEGELNSYLIEITRDIFSVVDKETGQYLVDLILDKAGAKGTGKWMSQLAL
                     DLGVPSTLVTEAVHARNLSALKEERVRASKILSGPPIQRYQGDRQEFIEQIRQALYAS
                     KIVSYAQGFVQLREAAKEHQWPLNFGSIAMLWRGGCIIRAVFLERIKEAFDENANLEN
                     LLLAPYFKQAVEKAQAAWRNVVCFALSNGIPIPAFATALTYYDGYRSERLPANLLQAQ
                     RDYFGAHTYERVDKPGQGPFHTDWLSERRPPKNIG"
     gene            2834..3618
                     /locus_tag="PKI05_06980"
                     /pseudo
     CDS             2834..3618
                     /locus_tag="PKI05_06980"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
BASE COUNT          868 a          904 c         1060 g          827 t
ORIGIN      
        1 gctatatggc gaccattctg cggaacccag gcccaatcta ttccgcacgc tacgataagg
       61 ttccgcttcg tgtggtggcc aatagcgaac gaaagttccc caaacagtgg atcgccgaga
      121 atggccacga tgtgacggat gactttatcc gttatgcgaa acccttaatc ggcgaggata
      181 tggtgaattt accgatggtt gacggtcgcc agcggatgac gcgactccag cccatttacg
      241 cggaaaagaa acttccgccc tatgtgccgc aggcagaccg cgggaagtga ataggatgtg
      301 tggagcggat ttccgccggt gtgatgacaa tcacgtgttt gccaacagtt gtcgttgatc
      361 gtggagtgcc ccatgatttc attgccccga cagaaagatt tcctggtagg tattgactcc
      421 gatggttgcg ctttcgatac gatggagctc aagcacaagg agtgtttcat cccaaacatc
      481 atttattact ataacctcca aggcgtgagc aaatacgcca gagaggttgc cgagtttgtc
      541 aatctctatt ccaagagtcg cggcattaat cgttttccgg cactgattga gacccttgag
      601 cgtctccggc ggcgtccgga agtaaaagca cgcggagtca aagttgtcgt cccggaaagc
      661 cttagaaaat ggatgcaaag cgaaaagaag cttggcaatc ctgccctgga gcaggctgtt
      721 caagcgacaa acgatcccga gctcaagcag gcactggagt ggtctcgggc ggtcaatcgt
      781 tcggtagaat ggatggttca tggagtgccg ccgttcccat ttgtccgcga gagccttgaa
      841 aaattatcca aggttgccga tatcatcgtg gtttcagcaa ctcccgagga agcgcttaaa
      901 agggaatggg ctgaacatgg attggataaa tatgttcggg caattctggg tcaggaaagc
      961 ggcacgaaga aggatttcct gggccttagc tctcagtatc cgagtggcca cagtttgatg
     1021 gttggggacg ccccgggcga ccaaaaggca gcggaagcca acggtgccct tttcttcccg
     1081 atcaaccccg gcgaagagga aaagagctgg caagaattct tcgaaaaagg tatcgaccgg
     1141 ttcctcaacg gaagtttttc cggcgattat caaaaggagc ttctcgcaca tttcgatcaa
     1201 tgtctgccca tcgatccgcc ttgggtcgag gaagccgact gattcaactt agggtccaat
     1261 ttcagaaacc atcgatttag gccaatcttg tgatgtgagg tgcgaacatg aacggtcaat
     1321 gtgatattgc ggtgatcggc ctggaggtta tggggcgtaa cttggcgctc aacatcgaaa
     1381 gccgcggcta tcgagttgcg gtctataacc gcactgtgga gaaaatggag aaattcatcg
     1441 aggggcctgc caagggaaag aacattgttg gttgtcgctc cctcgaagag cttgtcgcag
     1501 cccttgccaa gccgcggaag gtcatgatga tggtcaaggc gggaccggct gtcgatgcct
     1561 tgattgacca gttgatccca cttctggagc cgggtgatat cttaatcgac ggtgggaata
     1621 cccatttcgc cgacaccgaa cggcggacgg cgtacgtgga atcgaagggc ttgctctata
     1681 tcggaacggg tgtttccggc ggcgaggaag gtgccctcaa aggaccaagt ttgatgccgg
     1741 gcggcagtga aaaggcctgg cccatcgtca agcctatctt tcaggccatc gcggccaagg
     1801 ttggcccgaa taaggatatc ccctgctgtg attgggtggg gccccgtgga gccggccact
     1861 acgtcaaaat ggtccacaac ggtatcgaat acggggatat gcaactgatt gccgaggcgt
     1921 actggctcct caaacacggg ctgggggcta caaacgaaga attgtacgat attttcgctc
     1981 gctggaacga gggggaactg aatagctatc tgatcgaaat cacacgcgac atcttcagtg
     2041 tggtggataa agaaacgggt cagtacctcg tggacttgat cttggacaag gctggtgcca
     2101 agggaacagg caagtggatg agccagcttg ctctcgacct cggggttcca agtacgcttg
     2161 tcaccgaggc agtccatgca cgaaacctct cggccttaaa agaggaacga gtgcgtgcct
     2221 ctaagatttt gagtggtcca ccgatccagc gctatcaagg cgaccgacaa gagtttatcg
     2281 aacaaattcg tcaggccctt tatgcctcca agattgtcag ctatgcccaa ggatttgtgc
     2341 agttacgaga agcagccaaa gagcaccaat ggccgctcaa ttttggcagc atcgccatgt
     2401 tgtggcgggg cggctgtatc attcgggccg tgttcttgga acgaattaaa gaggcatttg
     2461 atgaaaatgc caatctcgaa aacctgctcc tggccccgta cttcaagcag gctgtcgaaa
     2521 aggcccaagc tgcgtggcgc aacgtcgtgt gctttgccct cagtaacggt attcctatcc
     2581 ccgcctttgc cacagcgttg acgtattatg atggttatcg ttcggaacgc ttgcctgcca
     2641 atctgttaca ggcgcagcga gactacttcg gagcgcatac atacgagcga gtggataagc
     2701 cggggcaagg accgttccac accgattggc tgtcagagcg acggccaccg aagaatatcg
     2761 gctaattttg cccaggttgc caaatcgtgg cataaaacgt gatgactggc gtgctaatgg
     2821 cgaggacata accatgacaa ctgcagaata tctggaggat ttgttcgggc ttcacggcca
     2881 agttgccgtg gtgattgggg gtgccggaga attgggcggt gccttgtgcg aggggatcct
     2941 gcgagcggga gcccatgtgg tcattgccga ccttcttgaa gaagcgtgcc gccgccgtct
     3001 ggagcaactc cgcgaattca gcgacaaggt cagttactgt cttgtcgatg tgacaaagcg
     3061 tgagtcgctg gagaacctac tcgagtttgc caagaagatc aagggacgcg tcgagattct
     3121 ggtcaattgt gccggaatca acgcgggaag ctcgtttctc gaggctagtc ccgaaatgtg
     3181 ggataaaatc atggcggtca atctcaaagg ggtctttgaa gcgtgccagg ttttcattgc
     3241 ccacatgttg gaagcaggtg gcgggtcgat cctcaatatc ggctctgtca cgtcaacatt
     3301 gccgctttcc cgtgtctttg cttatgccgc ctccaagcgg cggtggtcaa cctcaccaag
     3361 aatatcgcta atgagttcgg agataagggt attagggtca atgcaatttg cccgggcttc
     3421 ttccctgcag aacagaatcg ccgacttctc gacgaggaac gggtccgcaa tatcattaac
     3481 ggcacgccca tgcatcgctt tggagagccg catgagctga tcggggccgc cctgttgctt
     3541 ctgtcaccaa aagccggaag ttacattacc ggtgccgcgt tccacgtcga cggtggcttt
     3601 actgctgcgt ggttctaatt cagcggaggt gattttacgc gggacggctc aagctgaaa
//
LOCUS       DAOPTR010000308         3633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_20469_length_3633_cov_3.652320, whole genome shotgun sequence.
ACCESSION   DAOPTR010000308 DAOPTR010000000
VERSION     DAOPTR010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20469_length_3633_cov_3.652320"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /locus_tag="PKI05_06985"
     CDS             <1..1377
                     /locus_tag="PKI05_06985"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOA51984.1"
                     /translation="TLNEKPIRADVLGWYVYRYSDDFGRTWSERYRLPMRVTACDRTN
                     DWKGSVQLFWGICKPQVAERRVLFSFTKLGKYMLELGEGWVFCSGNILEEREPTRLQW
                     ELFPPGDRGIRNDQFGSVQEEHNLVWLGENTWYCVYRTTLGYPAQTYSFDGGKTWEVP
                     SLMTYEPGGRPMKNPRACPKLYRTSEGRLLFWFHNHSGTDFFGRNPAWLSAGTVKNGR
                     VYWSQPEILLYDPDPQVRFSYPDFFEDEGRMFVTETQKTVARVHEIDREFLNVLFQQG
                     TLASLPKVAPLVEIRSESPEGPKPLTRTIDLAEDSGFSVEMWIRLDHSQPGRILVDNR
                     DATNRGFVISLGEENSYQVELNDGLHSFAWSSDPGTIQVGKRQHVVIIVDSGPKLMMF
                     VVDGQLCDGGEHRQYGWARWTDDLRQVTGQSIWINPALKDCIHSLRIFGRPLRVSEAV
                     SLYRAGSH"
     gene            1636..2337
                     /locus_tag="PKI05_06990"
     CDS             1636..2337
                     /locus_tag="PKI05_06990"
                     /inference="COORDINATES: protein motif:HMM:NF020771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HOA51985.1"
                     /translation="MNDSPQGSKGRRSPKKRGDARQRLLEAGIEAFARFGPDEVGIRR
                     LAAAAGVNSAALSYYFGGKQQYYRAVLRYLMRWVGQSIRQAAMTARAGLAASPDACHA
                     RQLLRIFMREVVQTVLANPQAEAVAAIIWRELLRRSSGFDIVYEQMIRPVHEIITELV
                     AAAMGVTSDDREAVLLAHSLWGQVAIFRLGFHVLRRRLKIRGRQLSNEWVETITSTVD
                     LVVDRLLSSPPETRR"
     gene            2389..3456
                     /locus_tag="PKI05_06995"
     CDS             2389..3456
                     /locus_tag="PKI05_06995"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOA51986.1"
                     /translation="MKKRLILIVIIVAIVALSLWSLQRYARPTVDQGLHVQAWWTAIM
                     NGGTPEYRELHGNVEIREVRIGFKVPGRLARLYVDEGDTVGRGSLLAELETAEFLDAV
                     EQAEAALAARRAELLALENGSRPEEIEKAKSLAEAARVAVRNAEVSLRRAKDLAPKGA
                     VSQETLDNAQAAYDQAVANYQAALATQRLVEVGPRQEDIDRAKALVRQAEAVLKEAQR
                     RLADTRLISPIHGVVQVRIYEVGDYVGTGAPVFSIARQDEVWVRTYVAERELDHIRPG
                     TVVEVLTDGGNRFSGQVGFISSVAEFTPKNVETREVRTTLMYRVRVLVKDPSGKLRHG
                     MPVTVRIRRDETTEASTGRQR"
     gene            3453..>3633
                     /locus_tag="PKI05_07000"
     CDS             3453..>3633
                     /locus_tag="PKI05_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011118321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOA51987.1"
                     /translation="MIRFDHVTKRFSPKQRPALRDVTVEILAGAITGLVGPDGAGKTT
                     FLRLAAGLLMATEGRI"
BASE COUNT          788 a          917 c         1117 g          811 t
ORIGIN      
        1 acgctcaacg aaaagcccat ccgtgcagat gtgttgggtt ggtatgttta tcggtattca
       61 gatgactttg ggcgcacgtg gtccgagcga tatcgccttc cgatgcgcgt cacggcgtgc
      121 gaccgcacga acgactggaa ggggagcgtc caattatttt ggggaatctg caaaccgcaa
      181 gtggccgagc gaagggtcct gttttccttc acaaaattgg gaaagtatat gttggaattg
      241 ggcgaaggtt gggtcttctg ctctggtaac atcttggagg aacgtgagcc gacccggttg
      301 caatgggaat tatttccacc tggtgaccgc gggatccgta atgatcagtt tggctccgtt
      361 caggaagagc acaatcttgt atggctcggc gaaaacacat ggtactgtgt ttaccgaacc
      421 acgctgggct atcccgccca gacctatagc ttcgatggcg gcaagacatg ggaggtgccg
      481 tcgctcatga cttatgaacc agggggacgg cccatgaaaa acccccgggc ctgtcctaaa
      541 ctttatcgaa cgagcgaagg aagactctta ttctggttcc ataaccacag tggcacagac
      601 ttcttcggcc ggaatccagc gtggttgtcg gcgggcactg tcaaaaacgg tcgtgtctat
      661 tggtctcagc cggagatttt gctttacgat ccggacccgc aggtgagatt cagctatccc
      721 gatttctttg aagatgaagg tcgcatgttc gttaccgaga cgcaaaagac agtggcccgg
      781 gtgcacgaaa tcgaccgcga gtttttgaac gttctgtttc aacagggcac tcttgcctca
      841 ctccctaagg ttgccccact tgtggaaatc cgatccgaaa gcccagaggg ccccaaaccc
      901 ctgacgagaa ccattgacct ggccgaggat tcaggtttca gcgtggaaat gtggatcaga
      961 ttggaccatt ctcagccggg cagaattctt gtggataatc gtgatgccac gaatcggggc
     1021 tttgtgatct cgttaggtga ggagaattct tatcaagtag agctgaacga cggattacat
     1081 tcgtttgcct ggtcgtctga tccggggacc atacaggtcg gaaagcggca acatgtggtc
     1141 atcatcgtgg attccggtcc caagttgatg atgttcgttg tggatgggca gttatgcgat
     1201 ggaggagagc accgccaata cggatgggcc cgctggacgg atgacctgcg tcaagtcacc
     1261 gggcaatcga tttggattaa cccagcgctg aaagactgca tccattcact aaggatcttc
     1321 ggccgaccat tgcgggtatc cgaagcggtg agtctctacc gcgctggaag ccactgatgg
     1381 cctcaaatca gcgcccgctt atactgcctg actcgtagca agttgagtct gccgctgatc
     1441 gccactctgt ttgtcggcga atcttctgtg ccggtctcct agactaaggg ctcgcatcat
     1501 atagggaggg acctagaacc gttgtgtgtg atcgatcgtt ttaatcgatt gattaactca
     1561 attggttgac taatcgcctg atcagtggta tgatacagaa ggaggggtga ggtcgttttg
     1621 tgacggagaa ttgctatgaa tgatagcccg caaggctcca agggcagacg atcgccgaag
     1681 aaaagagggg atgcccgcca gcggctgctg gaggcaggga tcgaggcgtt cgcgcgattc
     1741 gggcccgacg aggtgggaat tcgtcgccta gcggcagcag ccggggtcaa cagcgccgct
     1801 ctctcttact acttcggcgg gaaacagcag tactatcgcg cggttctgcg gtacctcatg
     1861 cgatgggttg gtcagtccat tcggcaagca gcgatgaccg cgcgggctgg ccttgctgca
     1921 tctccagacg catgtcacgc ccgacagctc ttgcgtattt ttatgcgaga ggtcgtgcag
     1981 acggtgctcg ccaacccgca agcggaagcc gtcgcagcca tcatttggag agaattattg
     2041 cgccggtcga gtggctttga catcgtgtat gaacaaatga tccggccagt gcatgaaatc
     2101 attaccgagt tggtggcagc ggcgatgggg gtgacttccg acgaccgaga ggctgtcctt
     2161 ttggctcata gcttatgggg acaggtcgcc atttttcggc tgggttttca cgttttgcga
     2221 cgtcggttga agattcgcgg ccgacagttg tccaacgagt gggtggagac gatcacaagc
     2281 actgtggatc ttgtggtgga tcgccttctc tcatcccctc ctgagacgcg gcgatgaagg
     2341 tgtgtaatac gaacgcaaga acgcagttga aggcaaagca gtcccgttat gaaaaaacgc
     2401 ctgattctga ttgtgattat tgttgcaatt gtggctctgt cactgtggag cctccagcgg
     2461 tatgctcggc caacggttga tcagggattg catgtccagg catggtggac ggccatcatg
     2521 aacgggggaa cgcctgaata cagggagctt cacggaaacg tagagattcg tgaagtgcgg
     2581 attggattca aggttcctgg tcgccttgcc cggctttacg tggatgaggg ggatacggtt
     2641 ggacgaggtt cgctgcttgc cgagctcgaa acggctgagt tcctcgatgc cgttgagcag
     2701 gcagaggccg cgctggccgc acgtcgggct gaactcctgg ctttggagaa cggttcccga
     2761 ccagaagaaa ttgaaaaggc taagtcgctt gcagaggctg cacgggtggc agtccgaaat
     2821 gcggaagtgt cacttcgccg ggccaaagac ctcgcgccca agggggccgt ttctcaagaa
     2881 acgctcgata atgcccaggc ggcgtatgat caagctgtag ccaattatca agccgctttg
     2941 gccactcagc ggctcgtgga agtaggaccc cggcaagaag acatcgatcg cgccaaggcc
     3001 ctcgtccgtc aggcggaagc ggtactcaaa gaggcccaac ggcgattggc cgatacgcgg
     3061 ctgatttcgc cgattcacgg cgtcgtccag gtgcggatct acgaagtagg cgattacgtc
     3121 ggtacaggtg cgcccgtctt ctcgattgcc cggcaagatg aagtctgggt acggacgtac
     3181 gtggcggaac gagaattgga tcacattcgc ccgggaacgg ttgtagaggt cctcaccgat
     3241 ggaggcaacc ggttttcggg gcaggtagga tttatctcct cggtggccga attcacccct
     3301 aagaatgtcg agactcgcga agttcgcacc acgttgatgt atcgggtgcg cgtgctggta
     3361 aaagacccct cgggtaaact ccgccatggc atgcccgtga cagtccgcat ccgccgagac
     3421 gagacgaccg aggcaagcac cgggaggcaa cggtgattcg gtttgaccac gtgaccaagc
     3481 gtttctcgcc caagcaaagg ccggcgctcc gggatgtgac ggtggaaatc cttgctggtg
     3541 ctattaccgg cttggtgggg ccggacgggg ccggcaaaac gacgtttctt cgtctcgcgg
     3601 ccggactcct tatggccaca gaaggtcgga ttt
//
LOCUS       DAOPTR010000309         3632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_20474_length_3632_cov_4.327928, whole genome shotgun sequence.
ACCESSION   DAOPTR010000309 DAOPTR010000000
VERSION     DAOPTR010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20474_length_3632_cov_4.327928"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PKI05_07005"
     CDS             <1..699
                     /locus_tag="PKI05_07005"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOA51988.1"
                     /translation="EIEREAKTSSSSLSFWRIVRAGGTVGFLIILLSIVAVALAVEHL
                     LTLRRDLFMPLGLIEEVSAKLSAKDWNGAAEACRAHPSVLAHILISGISEHGAGWNEM
                     EKVMEETLAEQTARWNRKVEYLSVIGNIAPMLGLLGTVIGMILAFRTVAETQGAARAA
                     DLAEAIYLALVTTVEGLIVAIPSLAAYAFFRNRVDELMAEVAATVQKIFAPLKRRRAT
                     LAPAPAPPSAGASH"
     gene            699..1127
                     /locus_tag="PKI05_07010"
     CDS             699..1127
                     /locus_tag="PKI05_07010"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOA51989.1"
                     /translation="MPMHAVRGTHQPKVRLNLTPMIDVTFQLILFFLLAGHMAQQESQ
                     SDLALPYARTGEKLVEGDTRRLVINVLANGQIVLSGESLSVERLVQVLASEAGAGRRV
                     EVRIRTDKETPYKFISPILLTCARAGLWNVSFAVISKEEE"
     gene            1159..1605
                     /locus_tag="PKI05_07015"
     CDS             1159..1605
                     /locus_tag="PKI05_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOA51990.1"
                     /translation="MKRPVAYHHASHLDLPMTPMIDVVFQLIIFFLCTTRFTPLEHVL
                     PMHLDLPGAKGAVTKVSPDLQDLTEIVIILEAHQDTVGISVNRRPVASMAELRSTLMT
                     LGKIRLDVPIVIDPSPEVRISRVIEVYDLCREIGFVNIQFATPADR"
     gene            1602..>3632
                     /locus_tag="PKI05_07020"
     CDS             1602..>3632
                     /locus_tag="PKI05_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51991.1"
                     /translation="MKCLLPFLLLLAIPNAWGQTERLYYPRQREDYIFLQGLRQRHLY
                     HLAECYCKDKLLRGDLSLPERAILTSELIVTLNEWGMETEDSNERQSRWQEAISVAEA
                     FYRRFPTSDWRFIVALMDLKYRITVASAIREEMQLGTAYTEAISDASFLDEIRSILRK
                     TINALKDREAELQEVFRRPKSGSAKDDGSVFQEQQWLSLEKHLQFELARGFAELARTY
                     PAKSEDHTAGMVEAARRLEVLSQLPLDHPLGVPSRLALARILRELGDGARSTDILKGL
                     AEEKLSLDERFALRVELIRLALATQQKEELDKLLAMGRLIEGKTAPQLDNAFLEAFLE
                     KWKNARANNSDEARAWEAKIREILNGLRQNGPAFWARRAELLVTQQIAGNQVHQNIDM
                     NVYLAENAYRAKRWEEALAAFDRARKAAEQQGNPVRALQLGLAAAAIEHERERHQEAW
                     RRYQDLYRSFSDQEGAKDAAELAIFHVGQLAKENPTEFLPLYQQELENYLRMWPQSEF
                     ACQAALYLGKIHNHFGHRIDAANYLLLSLESWLQHIAAPEPQKREAAPIVPDTPSQSQ
                     TSQYSVIQTVSELETVTARILQDESQRPDTRQRLRELAQRLATWAKQARAHAPEEDWS
                     LRVASIAAQLLLKGENDPHSADAVLKQFLPEPLDTIDHLDVTVKARSITLKLE"
BASE COUNT          825 a          978 c         1010 g          819 t
ORIGIN      
        1 gaaatagagc gggaggccaa gactagttct tcttcgcttt cgttctggcg gattgtgcga
       61 gccggtggaa cggtaggatt cctgattatt ttgctgtcga ttgtggcagt ggctttggcg
      121 gttgagcatc tcttgacgtt gcggcgggac cttttcatgc cgctcggctt gatcgaggag
      181 gtctctgcca agctttcggc aaaagattgg aatggtgccg ctgaggcgtg ccgagcccac
      241 ccctcggtgc tggcacatat ccttatatcg gggatctcgg agcatggggc aggctggaac
      301 gagatggaaa aggtcatgga agagaccctg gccgaacaga ccgcccgttg gaaccggaag
      361 gttgaatacc tctccgtcat cgggaacatc gcaccgatgc tcggactttt aggcacggtc
      421 atcggcatga ttctggcgtt ccgcacagtt gccgaaacgc agggtgcggc gagggcggcg
      481 gacttagcgg aggctattta tctcgccttg gtgaccacgg tggaaggcct cattgtggcc
      541 attccgtcgc tggcggccta cgcatttttc cgtaatcggg tggatgaact tatggcggaa
      601 gtggcggcta cagtccagaa aatctttgcc ccactcaaac ggcgtcgtgc cacgctggcg
      661 ccggcccccg ctcccccctc ggcaggagca agtcattgat gcccatgcat gctgttcgag
      721 gaacacacca acccaaggtg cgtttgaacc tcacgccgat gatcgacgtg acatttcagc
      781 tcattttgtt tttcctcctt gctggacata tggcccagca ggaaagccaa agcgacctgg
      841 cactgcctta tgcccgaacc ggtgaaaagc ttgtagaagg cgacaccagg cggttggtga
      901 tcaacgtcct tgcgaacggc caaatcgtcc tatccggaga atccctctcg gtggaacggc
      961 tggttcaagt tcttgcatcc gaggccggcg ccggacgaag agtagaggtg cgaattcgta
     1021 ctgacaaaga gacgccgtac aagtttattt cgccaatttt gctgacatgc gcccgggcag
     1081 gcctttggaa tgtgtccttc gcagtgatct caaaagaaga agagtaatta ccggttggcc
     1141 cgtgaaattc gatcaatcat gaagcgaccc gtcgcgtacc atcatgcttc ccacctggat
     1201 ttgcccatga cgccgatgat cgacgtcgtg ttccagctca ttatcttttt cctttgcacc
     1261 actcgattca cccccttgga gcatgttctt cccatgcatc ttgacttacc cggggccaaa
     1321 ggggcggtga ccaaggtcag tcccgatctg caagacttga cggagatcgt catcatttta
     1381 gaagctcacc aggacacggt gggaatcagt gtcaatcgtc gtccggttgc cagcatggcg
     1441 gaattgcgtt ccacactgat gacacttggt aaaattcggt tagacgttcc catcgtgatc
     1501 gatccctctc ctgaggtccg catttcccga gtcattgagg tttatgacct gtgccgagaa
     1561 atcgggttcg tcaatatcca atttgcgacc ccagccgaca gatgaaatgc ttgcttcctt
     1621 ttctcctttt gctggccatt cccaatgcgt gggggcagac agagcggttg tactacccgc
     1681 ggcaacggga ggattacatt ttccttcaag gtctccggca gcgtcatctc taccatttgg
     1741 ccgagtgtta ctgcaaggac aagctgctaa ggggcgatct ttcccttcct gagcgggcca
     1801 ttctcacatc tgaattgatc gtgaccctga acgagtgggg catggaaacc gaagactcga
     1861 acgaacgtca atcccgttgg caggaggcca ttagcgttgc cgaagcgttt tatcgccgtt
     1921 ttcctacatc cgattggcga tttatcgtag cgctcatgga tttgaaatat cggataacag
     1981 tggccagcgc gatccgcgag gaaatgcaac tgggaactgc ttataccgaa gccatttcag
     2041 acgcgtcttt ccttgacgaa atccgttcga ttttacggaa gacgatcaac gccctaaaag
     2101 accgcgaagc cgaattgcag gaggtattcc gtcgtcccaa gagtgggtcg gcaaaggatg
     2161 atggctccgt ctttcaggag caacaatggt taagtctgga aaaacacctg cagttcgaat
     2221 tagctcgcgg atttgcagag ttagcgcgaa cctatcccgc gaagagtgag gaccataccg
     2281 cgggcatggt ggaggcggct cgacggcttg aagtcctttc gcagttgccg ctcgaccatc
     2341 cgctgggtgt cccatcccgt ttggcgctag cgaggattct tcgcgagttg ggcgacggcg
     2401 cgaggagcac tgatatctta aagggccttg cggaagagaa gttatcgctt gacgagcgat
     2461 ttgccctccg agtcgaactt attcgtctgg ccctggcgac tcagcaaaag gaggagttgg
     2521 ataaacttct cgccatgggt cggctcattg agggaaagac cgccccacaa ctcgataacg
     2581 catttcttga ggcttttctc gaaaaatgga aaaacgctcg cgcgaacaac tcggatgaag
     2641 cccgcgcctg ggaggccaag attcgagaga ttctcaatgg cttgcggcaa aatggtcccg
     2701 cgttttgggc gcgacgggcc gagttgttgg tgacgcagca gattgcgggg aaccaagttc
     2761 accagaacat cgacatgaat gtctatcttg ccgaaaatgc ctatcgtgcc aagcggtggg
     2821 aggaagccct ggccgctttc gaccgggcgc gcaaggctgc cgagcaacag ggaaaccccg
     2881 ttcgcgctct gcagttaggc ctggccgcgg cggccattga acatgagagg gaacggcatc
     2941 aagaggcttg gcggcgctac caggaccttt atcggagttt ctcggatcaa gaaggggcta
     3001 aggacgcagc agagctcgct atttttcatg tggggcaatt ggccaaagag aatcccaccg
     3061 agtttctgcc gctttatcaa caggagctcg agaactatct tcggatgtgg ccccaatcgg
     3121 agttcgcgtg tcaggctgct ctttatctgg gcaaaattca taaccatttt ggccaccgga
     3181 tcgatgcagc caactatctt ctgttatccc tagaatcttg gttacaacac attgcggccc
     3241 ccgaacccca gaaacgagaa gcagcgccga tcgtcccgga cactccatcg caatcgcaga
     3301 caagccaata ttctgtcatt caaaccgttt cggaattgga aaccgtcacg gcgcgaattc
     3361 ttcaggatga gtctcagcgg cccgacacaa gacagcgact acgagaactc gcccaacgtt
     3421 tagcaacctg ggcgaaacag gcaagagcac acgcacctga agaggattgg agcctgcggg
     3481 tggcaagcat cgccgcgcag ttgctgctaa aaggcgaaaa cgatcctcac tcggccgacg
     3541 cagttctcaa acaatttttg cccgaaccac tcgataccat tgatcacctc gatgtcacgg
     3601 tcaaggcaag atccattacc cttaaattag aa
//
LOCUS       DAOPTR010000310         3631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_20481_length_3631_cov_2.795861, whole genome shotgun sequence.
ACCESSION   DAOPTR010000310 DAOPTR010000000
VERSION     DAOPTR010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20481_length_3631_cov_2.795861"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PKI05_07025"
     CDS             complement(<1..614)
                     /locus_tag="PKI05_07025"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOA51992.1"
                     /translation="MIIKLCQIIWTFAQIGLVSFGGPAASIAIMQRTIVDRRKWVTQS
                     EFLDFVAVSHMIPGPIAVQLSLHLGYRRGGLLGALAAVFGFVVPAAFITWAVAVFYQR
                     SHALPALEACLAGVRPAVLAVILISLWDIAGRLTWNKWRFVLLAAVVGLNLVGIDAIV
                     IMALTLAYSAFYLSVTGRKSRGQGKAVETNSEKGQPPAQGPNLFG"
     gene            802..1647
                     /locus_tag="PKI05_07030"
     CDS             802..1647
                     /locus_tag="PKI05_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOA51993.1"
                     /translation="MKSANDRREGDVEYEFGIPIPGRILPEDQWARTALKKLPPEGPI
                     DWAQVFGRTAPLILDIGCGNGRFTLASAIRRPEFDHVGIDILPVVIRYATRRANQRGL
                     WNVRFAVCGGHEFLDRYIAPHSVQEIHVYHPQPYKDPRKQHKRLLSPEFLALVFRSLF
                     PDGLFVLQTDNAAYWRYVRPLMDKFFNFHDQEGPWPDMPEGRTRREIYARLKGLPVFR
                     GYGTPRTDLSDTQAMELAKQLPPPVFSAAEKADPLAVAGRPRRSTARSFRQKRAEKWR
                     KRRNS"
     gene            complement(1727..3136)
                     /locus_tag="PKI05_07035"
     CDS             complement(1727..3136)
                     /locus_tag="PKI05_07035"
                     /inference="COORDINATES: protein motif:HMM:NF014398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HOA51994.1"
                     /translation="MSEAKGRQENSLMWIGWAVFFVLLIITFALGLLVSSIVTRRKEA
                     IPLPLPRPIARWESDSRKWADAFPREYDRYRMMMESSTKTKYGGAFPRDYLEETPANV
                     ILFAGYAFAKDYLQARGHVYAIHDVTHTKRVNEKTPATCWTCKSTDVPRILAEMSPAE
                     FYKQPFSAFKDQITHPIGCLDCHDPTTMRLTITRPALREALQRQGRDLENIPHQEMRT
                     LVCAQCHVEYYFKGEGKYLTFPWDKGFRIEEIESYYDEAEFSDWTHAISQAPMVKLQH
                     PDYEVYLTGIHAYRNIACADCHMPYHSEGGVKFTDHYIQSPLLNIANSCAVCHRWSEE
                     EIRQRVESIQDKTKEVRQRVEETLCKAHFEVAAARQAGATDDELQNPRQLIRKAQLRW
                     DFVAASNGMGFHAPGECLRILAAALDLGSQARTELARILATRGVVKPIIYPDISTKAA
                     AQQVEQSFVSGNPPNLLPK"
     gene            complement(3236..>3631)
                     /locus_tag="PKI05_07040"
     CDS             complement(3236..>3631)
                     /locus_tag="PKI05_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51995.1"
                     /translation="SWRHSSHAEVAHCNDCHVPHDSFVNHWLFKARDGLWHATVFTMR
                     WEPQVIHLSERAVPVVESNCRRCHERLVACTSLREHTAGDLRCWDCHGDVPHGETRGL
                     ASSPGYLDPSLPPVGPRSKGVTIGGRPPR"
BASE COUNT          820 a          991 c          996 g          824 t
ORIGIN      
        1 ccaaacaggt tggggccttg ggcgggaggc tggccttttt ccgaattcgt ttccacggcc
       61 ttgccctggc cacgtgattt acgacccgtc acacttaagt agaacgcact gtacgcaagg
      121 gtgagtgcca tgataacaat ggcatcaatt cccacgagat ttaagccaac gacggccgca
      181 agcagtacaa atcgccactt gttccaagtg agccttcccg cgatgtccca aagcgaaatt
      241 aggatcacag cgagaacggc aggccgaacg cccgccagac aagcctccag tgcaggcagg
      301 gcatggctac gctgatagaa aacggcaacc gcccaggtga tgaatgcagc cggcacaaca
      361 aagccaaaca ctgcggccag tgctcctagc agtccgccac gccggtagcc aagatggaga
      421 gaaagctgaa ccgcaatggg acccggaatc atgtggctga cggccacaaa gtcaagaaat
      481 tccgattgcg tcacccattt acggcgatcg acgatggtcc gctgcattat cgcaatggaa
      541 gcagccggac caccgaagct gacgaggcca atttgcgcga acgtccatat gatttgacaa
      601 agcttgatga tcattttggc tcggcacgtg atgccgtact cgagttcggt agtcaacgtt
      661 tggaaaaagc ctccggaggt tgtgttttgc ctggtctttg cgatgaagta ttgattttcc
      721 cggcgcgtga ttcggcctgg gacaatggca gcaattgtag gacatcattg gcgcatcgcc
      781 aatgggatgg agtgacccct tatgaaaagt gccaatgacc gtcgcgaagg tgacgtggaa
      841 tacgagtttg ggataccgat ccctggcagg attttgcccg aggaccagtg ggcaagaacc
      901 gccctcaaga aactgccccc agagggaccc atcgattggg cacaagtgtt tgggcgtacc
      961 gcacctctga tcctcgatat tggatgcggc aacggccggt ttaccttggc cagtgcgatc
     1021 cgccggccgg agtttgacca cgttggtatt gatattttgc cggtggtaat tcgatatgct
     1081 acacgtcgcg caaatcagcg aggattatgg aacgttcgtt ttgctgtgtg cggtggccat
     1141 gagtttctcg atcgttacat tgcgccacat agcgtgcagg aaattcacgt ttaccatccc
     1201 cagccctaca aggatccaag aaagcaacac aaacgactcc tctcgccaga atttctcgcc
     1261 cttgtctttc gctccttatt cccagatggt ttgtttgtgc tacagacaga caatgccgcc
     1321 tattggcggt atgttcgccc actgatggac aagttcttca acttccatga tcaagaagga
     1381 ccctggccgg acatgcccga ggggcggacc cgacgtgaaa tctatgcgcg actcaaaggg
     1441 ttgccggtgt ttcggggata cggtaccccg cgcacagacc tgagcgacac ccaggccatg
     1501 gagttagcca aacagcttcc tcctcccgtc ttttctgctg cggaaaaagc cgatcctctg
     1561 gccgttgccg gtcggccgag acgatccacg gcccggtcgt ttcgtcaaaa aagagcggaa
     1621 aagtggcgaa aacggcgtaa ttcgtaacct gtcgtgcatc aaacacctcg cagcctgcga
     1681 ccaagccagg aggttaatgt ttcgcctagc caagtcgcgc gtgcgatcat ttcggcaata
     1741 agttgggcgg attgccgctt acgaagcttt gttccacctg ttgggcggcg gctttagtgg
     1801 agatgtcagg atagatgatg ggtttaacca ctccgcgcgt ggcaaggatc cgagccaatt
     1861 ctgttcgtgc ctggctcccc aggtctaagg cggcggccag gattcgtaaa cactcgcccg
     1921 gtgcatggaa acccatcccg ttgctcgctg ccacaaaatc ccatcgaagt tgggctttac
     1981 ggatcaattg ccgcggattt tgcagctcgt cgtctgtggc cccagcctgc cgggcggcgg
     2041 cgacctcgaa atgggccttg caaagggtct cctcgacccg ctgacgtacc tctttcgtct
     2101 tgtcttgaat gctttccacc cgctgacgaa tctcttcttc actccaacgg tggcacacgg
     2161 cgcaggaatt ggcgatgttg agcaggggac tctggatata atggtcggtg aatttcacgc
     2221 cgccttcgct gtgataaggc atgtggcagt cggcacaggc tatgtttcgg taggcatgaa
     2281 tcccagtgag atacacttcg taatcgggat gttgtaactt gaccatcggc gcctggctaa
     2341 tcgcatgcgt ccagtcgctg aactcggcct catcgtaata agattcgatc tcttcgatgc
     2401 ggaagccttt atcccacgga aatgttaagt atttaccctc gcctttgaag tagtactcca
     2461 catggcactg ggcacacacc agcgttcgca tttcctgatg ggggatattt tccagatcac
     2521 gtccttgtct ttgtagcgcc tcgcgaagcg cgggtcgcgt aatggtcaac cgcatggtgg
     2581 ttgggtcatg acaatcgagg cagccaatgg gatgagtgat ttgatctttg aaagccgaga
     2641 acggctgctt gtaaaattcc gctggagaca tctccgccaa aatccggggg acgtccgtgc
     2701 ttttgcaggt ccaacatgtt gccggcgtct tttcattgac tcgctttgtg tgagtcacgt
     2761 cgtggatagc gtagacatgt ccgcgggctt gaagatagtc tttggcaaag gcgtaaccgg
     2821 cgaagaggat cacattggct ggagtctcct ccaagtaatc gcgggggaag gcacccccgt
     2881 atttcgtttt cgtagaagat tccatcatca tccgatagcg atcgtattct cgcggaaaag
     2941 catcggccca tttgcgactg tccgactccc aacgcgcgat gggacgcggt aggggcaaag
     3001 ggatggcttc tttgcgccgt gtcacaatgg atgagacaag tagtcccaag gcaaaggtga
     3061 taataagcag gacaaagaac accgcccaac caatccacat caagctgttt tcttgacgac
     3121 cctttgcttc gctcatggtt ttacatccct cgcagcgaaa tgtgtcttct cgcagttaat
     3181 aaatcacgga ttcttcgtca attgaggcaa gtttacgctc tttcttgcaa aaagcctatc
     3241 gcggaggtcg cccgccgatt gtgacgcctt tcgatcgtgg acccaccgga ggcaagctgg
     3301 gatcgaggta ccccggggaa cttgccagtc cgcgtgtttc cccatggggc acatccccat
     3361 ggcagtccca acagcgaaga tcgcctgcgg tgtgttcacg aagtgatgtg caggcgacaa
     3421 gccgctcatg acaacgccgg caatttgatt ccaccacagg cactgctcgc tcagacagat
     3481 ggatgacctg gggttcccac cgcatggtaa aaaccgttgc gtgccacagg ccgtcccgtg
     3541 ctttgaatag ccaatgattt acaaacgaat cgtgaggaac gtggcaatcg ttacaatggg
     3601 ccacctccgc atggctgctg tgacgccaac t
//
LOCUS       DAOPTR010000311         3629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_20492_length_3629_cov_3.478456, whole genome shotgun sequence.
ACCESSION   DAOPTR010000311 DAOPTR010000000
VERSION     DAOPTR010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20492_length_3629_cov_3.478456"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PKI05_07045"
     CDS             <1..474
                     /locus_tag="PKI05_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA51996.1"
                     /translation="PLAGGRLEIRTQRPTPDGIMTKVTVVDAQYGLILAQYLYDPQGR
                     LVASAVSYDHRREPVTGLVLARTVEINVPASGMVLRLNLGNARINEPIPNPSLTWNMP
                     VYPGYPMVNLADPRNLSGISVVACPSRGTAQGPVAPFTGVEGGSGQGLTWLPIPQ"
     gene            612..1508
                     /gene="folD"
                     /locus_tag="PKI05_07050"
     CDS             612..1508
                     /gene="folD"
                     /locus_tag="PKI05_07050"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007331358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOA51997.1"
                     /translation="MSAQLIDGKAIAQAIREEIRLGVERLRQEHGVVPGLATVLVGDN
                     PASVSYVTAKNKACAELGMASFQHTLPADITEEKLLALIDQLNRDASVHGILVQLPLP
                     KGICESRVIEAIDPRKDVDGFHPVNVGRLMLGDPVFPPCTPAGIQALIIRSGTPIEGA
                     EVVVVGRSNIVGKPVAVMLLQKQRGANATVTVCHTQTRDLAFHTRRADILIVAAGRPQ
                     AITGDMIRPGATVIDVGVNRVGYTESGKVKLVGDVDFDSAVKVAGKITPVPGGVGPMT
                     ITMLMRNTLRAARLHLGLPLDG"
     gene            1719..1943
                     /gene="infA"
                     /locus_tag="PKI05_07055"
     CDS             1719..1943
                     /gene="infA"
                     /locus_tag="PKI05_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOA51998.1"
                     /translation="MAKEKEKGIEVPGVVTEALANTNFRVQLDTGHTILAYVAGRMRK
                     NFIRIVPGDRVRVEISPYDPTKGRITYRER"
     gene            2361..3443
                     /locus_tag="PKI05_07060"
     CDS             2361..3443
                     /locus_tag="PKI05_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOA51999.1"
                     /translation="MSHLPADERTAISADEFLALHRQVAALISLGLPIERHLAGWNRR
                     QGRRLKRATRQVISSLQKGQTLSEALSQVDKDLAPVYRAVLEAGMASGRPGMALQELT
                     ECGQRILGIRDLLLSSLYYPLIVFLVAWAVVILYLLVPFPVLHEVIISWNGESAVFDW
                     LNQARATMHYWGPIGPVVILLLFLLWRFQLRKSKLLIPWRAAARLGWFPGVRRLIQWQ
                     QNVVLSDMLALMTRQGVPLDRAVRLAGAIFADPKMERESGAMADAIAAQDWQRVLEVR
                     GARRIPMSIRLAIVEGVRSGRLEDALMRVWQHWRRQSWRQDVWIRTIAVPLVVVAFGC
                     VCVLIVALTFWWPWLQVMFQMFSFRP"
BASE COUNT          704 a          894 c         1146 g          885 t
ORIGIN      
        1 ccgttggcag gagggcggtt ggaaatccga acccagcggc ccacacccga tggtattatg
       61 accaaggtaa cggtggtgga tgcccaatac gggttgatcc tggcccaata tttgtatgat
      121 cctcaggggc gattggtggc aagtgctgtc agttacgatc accggcggga acccgtgacg
      181 gggcttgtgc tagcgcgaac ggtcgagatc aatgtgccgg cgtccgggat ggtccttcgc
      241 cttaacctgg gtaacgcccg cattaacgaa ccgatcccca atccttctct gacatggaat
      301 atgccggttt atccgggtta tccgatggtc aaccttgccg atccgcgaaa cttgtccggg
      361 atcagcgttg tcgcttgccc ttctcgtggt accgctcagg gaccggtcgc cccattcacc
      421 ggcgtggagg gcggttcggg ccaagggctg acatggcttc ccattccaca gtaggataat
      481 ggaactgttg gcgtctaaaa gatgcccgga tccgtcggtg tgtgggaagc gtttatctgc
      541 gtggtcgtct ggctcatagg ataccttccc cggttaaccc taggttggtg tttagcatca
      601 ggagggaaat cgtgtcggcc cagttgattg acggcaaagc aattgctcaa gccatccgtg
      661 aggagattcg cttgggagta gaacgccttc ggcaggaaca cggagtcgtt cccggattgg
      721 ccaccgtttt ggtgggtgac aacccggctt cggtgtcgta cgtcacggcc aagaacaagg
      781 cgtgtgccga attgggcatg gcttcgtttc aacatacgct gccggcggat attacggagg
      841 aaaagcttct cgcgttgatc gatcaactta atcgagatgc ctcggttcac ggcatccttg
      901 tccagttgcc tttgcccaag gggatttgtg aatctcgggt gatcgaggcc attgatcctc
      961 ggaaggacgt ggatggtttt caccccgtca acgttgggcg gttgatgctg ggcgatcccg
     1021 ttttcccacc atgtacaccg gcgggcatcc aggcgttaat cattcgatcg ggaacgccca
     1081 ttgagggggc ggaggtcgtc gtcgtggggc ggagtaatat cgttggcaag cctgttgcag
     1141 tcatgcttct gcagaagcaa cgtggggcaa atgccacggt gacggtgtgc cacacccaaa
     1201 cgcgggattt ggcattccac acacggcggg ctgatatcct tattgtggcg gcgggacggc
     1261 cgcaggcgat tacgggcgat atgattcgac ctggggccac ggtgattgac gttggtgtga
     1321 accgagtggg ttacacagag tccggcaagg tcaagctggt gggagatgtg gatttcgatt
     1381 cggccgtgaa ggtggcggga aagatcacgc ccgttcccgg cggtgttggc cccatgacga
     1441 ttaccatgtt gatgcgaaac acgcttcgtg ccgcgcggtt acacctggga ttgccattgg
     1501 acggatgaac cggacgccac ggtgcacatg cctccaggaa ttcctcaatg gaggcccacc
     1561 ctgtcgtggc cggaccgttg gacacttgga gtcacgctgt ccacgtggta ggatgaaaca
     1621 atgagagcaa cgtgtccctc ctctgttttc actcccaagc ggtggtcgtt tgggcggtag
     1681 gaaatttgtt cgatgctgtc tttgggtgga aggtggttat ggctaaggaa aaggaaaaag
     1741 gtatcgaagt tccaggtgtt gtgacggagg ctttggcgaa caccaatttt cgagtgcaac
     1801 ttgataccgg tcacaccatt cttgcctatg tggccggacg catgaggaaa aactttatcc
     1861 gtattgtgcc gggcgatcgt gtgcgggtag aaatttctcc ttacgatcct accaaggggc
     1921 gaatcaccta ccgtgagcgc taaacggtgg ctatctcgtt tttgtctagt gcctcggtct
     1981 attggcctgt tcgcgctacg gccgaatctc tttccaggaa cgtaaagacc gttggtctgc
     2041 cggccgtata gcctcggtaa gtttccgtat aagactttct ttcggtatca cgccacccaa
     2101 tgactgtgga caagttgccc caggcggaga aaacgcttct gcgtggattc ccttaatgag
     2161 gaaaaggcat agcgacaaag aacgtcccac ggcggggtgc agtataccgt gctcgttcat
     2221 cctatttggc cctcccctcc ggatgattgg gtaaaaccag tttggcgttc gttgacaccc
     2281 gccgagtgcg tgtctatact acgtcatggt cttgcgattc cctggcgggt ttgtcgtggg
     2341 cgaactcgtg gagctaaaac gtgtctcatc tccctgctga tgagcggacg gcgatttcgg
     2401 cggatgagtt cttagcattg catcgccagg ttgcggctct catctcgctg ggtctaccta
     2461 tcgaacgcca cttggcaggc tggaatcgtc gccaggggcg tcgcttgaag cgagcaactc
     2521 gtcaagtaat aagtagcctg caaaaaggcc agacgcttag tgaagcgctc tctcaggtgg
     2581 ataaggacct cgcaccggtt tatcgggcgg tgctggaagc cggtatggcg agcggacgac
     2641 cgggaatggc ccttcaggaa ttaacagagt gtgggcaacg gattttggga attcgtgatt
     2701 tgctgctttc gtccctttat tatccgttaa ttgtgtttct tgtggcctgg gccgtggtca
     2761 tcctctacct gcttgtgccg tttccggttc tccatgaggt catcatctcc tggaatggcg
     2821 aaagcgcggt ttttgactgg ctcaatcaag cccgcgcgac gatgcattat tggggaccga
     2881 tcggcccggt ggtgatcttg ttgctctttt tgttgtggcg atttcaattg agaaagtcga
     2941 agctgctgat tccatggcgg gccgcggctc gattggggtg gttcccgggt gtgcgacggc
     3001 ttatccaatg gcagcaaaac gttgtcctct ccgatatgtt ggccttaatg actcggcagg
     3061 gggtgccgct ggatcgtgcc gtgcgtttgg cgggggcgat ttttgcggac ccaaaaatgg
     3121 agcgggaatc aggcgcgatg gcggacgcca ttgccgctca agactggcag cgggttcttg
     3181 aagtcagagg tgcgcggcgg atccccatgt ccatccgctt ggccattgtt gagggggtgc
     3241 gttcaggaag acttgaagac gccctgatgc gtgtctggca gcattggagg cggcaaagct
     3301 ggcgtcagga cgtttggatt aggacaatcg ctgtgccact ggtggtcgtg gccttcggtt
     3361 gcgtgtgcgt gctgattgtt gctttaacgt tttggtggcc gtggcttcaa gtgatgtttc
     3421 agatgttcag cttccgtccg tgacgcggtg agcttcggga aacggccagg ttgtctcttc
     3481 aggcaagaaa ggtctttagt tgggagggaa ggccatggcg aaagctcgtt tctgcgaaac
     3541 gtgtggcgat cccatcgagg aagattggtg gatgaaaagc gaatacatga tggaagcggg
     3601 gcaaatagcg cgggccgccg atagtgtga
//
LOCUS       DAOPTR010000312         3624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_20532_length_3624_cov_3.468759, whole genome shotgun sequence.
ACCESSION   DAOPTR010000312 DAOPTR010000000
VERSION     DAOPTR010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20532_length_3624_cov_3.468759"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..530
                     /locus_tag="PKI05_07065"
     CDS             18..530
                     /locus_tag="PKI05_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOA52000.1"
                     /translation="MEFDAALVPFTLFLMALVGLSIGLIVLGHLCGPHRANPVKAMPY
                     ESGVDPFHDARRRFHVRFHLVAIAFLVFDVELLFLYPWVVSLRQSSSWQSKAAALSSC
                     TETTGRSDAGVVVGLGSHESPSEQSGELATGALLQRSLQGILWPGLVFFVLLGLGYAY
                     DWRRGVFDWK"
     gene            583..1203
                     /locus_tag="PKI05_07070"
     CDS             583..1203
                     /locus_tag="PKI05_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOA52001.1"
                     /translation="MTDLPDNVVITKLDALVNWCRKNSLWPMPFATACCGIELMATGA
                     SRHDIARFGAEVFRFSPRQCDLMIVAGRVVMKMMPVLQRIWLQMHEPKWCISMGACAS
                     TGGVFDTYAVVQGIDRFIPVDMYVPGCPPRPEQLLQAIIDLQEKIQREGSAWGKDVER
                     RPHQPPRALLADPKNPRLLVAGPPIFAQDEAANKWDTGKRGESPDS"
     gene            1304..1801
                     /locus_tag="PKI05_07075"
     CDS             1304..1801
                     /locus_tag="PKI05_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOA52002.1"
                     /translation="MVSSETIQAIQARFPSAEITEFRKQFRVCVPREIVHDVLRFLKD
                     ECGFTLIIDITCVDYLYYPKAKDRFGVVYIVGRPENADRLIVKTFVNDPEPTLPTVTD
                     LWEGARWLEREVWDMFGIRFEGHANLRRILLPEDFQDHPLRKDYPLQGRGERHNIPVV
                     TRDMG"
     gene            1872..3092
                     /gene="nuoD"
                     /locus_tag="PKI05_07080"
     CDS             1872..3092
                     /gene="nuoD"
                     /locus_tag="PKI05_07080"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HOA52003.1"
                     /translation="MPLQRSELSQATDEVQEYLWTMNFGPQHPATHTTLRLILKLEGE
                     RVVDADCDIGYLHSGFEKLAEHLNYNQYVTITDRMNYVSPMANNVAWHLAVEKLMGLE
                     VPPRCQYLRVIVAELARIADHLVCNAAVGLDTGAFTFFMYAFNPREMIYDIFEALCGA
                     RFTNSYTRVGGLMYDASPKAIELIREFLRKMPRALDDMDRLLTRNRVFVDRTKGIGVL
                     TKEEAINRGVTGPIARASGVTRDLRKDEPYLAYSDFDFQVCCAQGGDCYARYLVRMAE
                     MRESLKIIEQAVENLPSGPVHVDVGQRAIIPDKWQVHSTIEGLITHFEIIMQNRGFEV
                     PCEEVYCATESPNGELGFYIVGDGSPYAYRARCRPPSFVHFALFPHLIRGHMLGDVVA
                     VLGSLNIIAAELDR"
     gene            3123..>3624
                     /gene="nuoE"
                     /locus_tag="PKI05_07085"
     CDS             3123..>3624
                     /gene="nuoE"
                     /locus_tag="PKI05_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOA52004.1"
                     /translation="MSQESKAQTVEGRGGALLPPEVIASIREYFPRYPNTRAVVLPAL
                     HLIQETLGYVPKSAVLELANVLGIPASDIEDTLSFYQFFKQDRPLGKYRIWVCRSLSC
                     ALCGGENLLDYLRERLNIEPGETTADGRFTLEAAECLGACEYAPAVLINETLYPTMTR
                     EKLESVL"
BASE COUNT          768 a          892 c         1072 g          892 t
ORIGIN      
        1 cttaaagcga ggaagcgatg gagttcgatg cggcactcgt tcctttcacg ctgttcttga
       61 tggccctggt gggtttgagt atcggtctca tcgtcttagg ccatttgtgt ggaccgcatc
      121 gggcgaatcc tgtcaaggcc atgccttacg aaagtggcgt ggatcctttc cacgatgccc
      181 ggcgtcgctt tcatgtgcgg tttcatctgg tcgctatagc gtttctggtc tttgatgtgg
      241 aattactttt tctttatcca tgggtcgtat cgctgaggca aagtagttcc tggcaaagta
      301 aggctgcggc cttgagttcc tgcacggaaa caaccgggcg gtccgacgcg ggagttgtgg
      361 tgggcttggg cagccacgaa agtccgtctg agcaatctgg cgagttggcg accggggcac
      421 tgctccaacg ctcgctccaa ggcatcctct ggccgggatt ggtgttcttc gtcctgcttg
      481 gtctgggata tgcctacgac tggcgacgag gggtatttga ttggaagtga ttggaagtga
      541 tggggggcaa catcctaatg gtggatgacc tcaatgacaa gaatgactga cttgcccgac
      601 aatgtcgtca ttaccaagct cgatgccttg gtgaattggt gtcgcaaaaa cagtctttgg
      661 cccatgccgt ttgcgacggc gtgttgcgga atcgaactaa tggccacggg tgccagccga
      721 cacgatattg cccgctttgg ggccgaggtc ttccgtttta gccccaggca gtgcgatctt
      781 atgatcgtgg cgggtcgggt tgtgatgaag atgatgccgg tcctgcaaag gatctggctg
      841 cagatgcatg agccgaagtg gtgtatttcc atgggggcct gtgcatcgac cggcggcgtc
      901 ttcgacacct atgccgtggt tcaagggatt gaccgattca ttcccgtgga tatgtacgtg
      961 cctggttgcc caccgcgtcc ggagcagctc cttcaggcga ttattgacct tcaggaaaag
     1021 atccaacgcg aaggttcggc ttgggggaaa gatgtggaga ggagaccaca tcagccaccg
     1081 cgggcacttt tggccgatcc caaaaaccct cgtttgcttg ttgccggtcc accgattttt
     1141 gctcaagatg aagcagcgaa taagtgggat accggcaaac gtggggagtc tcccgactcg
     1201 taggaatcgc ggcaatcacc gccatgacgt cttagccgtc ggtcgaattg ggtgtacaag
     1261 aggattgttt ggaatcggtg ttatctacgg aagtgactga aacatggtgt catccgagac
     1321 aatccaggcg atccaggcca ggtttccatc ggccgagatc acggagtttc gcaagcaatt
     1381 tcgggtttgc gtgccccgtg aaatcgttca tgatgtgctc cgctttttga aggacgagtg
     1441 cggctttacc ctgatcattg acattacgtg tgttgattat ctgtattacc cgaaggccaa
     1501 agatcggttc ggcgtggttt acattgtggg ccgtcctgaa aacgccgatc gattgatcgt
     1561 gaagacattc gttaatgatc cggaaccaac tctgcccaca gtgaccgatc tttgggaagg
     1621 cgcccgttgg ctggagaggg aagtctggga tatgtttggt attcgctttg aaggtcacgc
     1681 caatttgcgg cgtatcttgc tgcccgaaga ctttcaagat caccctctcc gaaaggatta
     1741 tccgcttcag ggacggggtg aacggcacaa cattcctgtt gtcacgcggg atatgggatg
     1801 aaggggcgtg tgtttccttc gggaaaagat taggttaaga ggacttcagg aaaaaggaaa
     1861 gccggaaaaa catgccgctt caacgaagcg aactctcaca ggcgacagac gaggtccagg
     1921 aatacctgtg gacgatgaat ttcggtccac agcatccggc cacgcatacc acgctccgcc
     1981 tcattctgaa attggaaggc gaacgcgtcg ttgatgccga ctgtgacatc ggttaccttc
     2041 attccggatt tgagaagctg gcggaacacc tgaattacaa tcagtatgtc acaatcaccg
     2101 atcggatgaa ttacgtatcg cccatggcca ataacgtggc ctggcacctt gcggtagaaa
     2161 agctcatggg gctggaggtc ccgccgcgat gtcagtattt gcgtgtcatt gtggcggagt
     2221 tggcgagaat tgccgatcac ctcgtctgta atgcagccgt tgggctggac acgggggcgt
     2281 ttaccttttt catgtacgca tttaatcccc gtgaaatgat ttacgacatt ttcgaggccc
     2341 tttgcggggc acggttcaca aacagctata cccgtgtagg tggcttaatg tacgatgcct
     2401 cgcccaaggc catcgaattg attcgcgagt tccttcgcaa gatgcctcgg gcattggatg
     2461 atatggaccg actgctcacg cgcaatcggg tatttgtgga tagaaccaaa gggatcgggg
     2521 ttctgaccaa ggaggaggcg atcaaccggg gcgttacggg gcccatcgcg cgtgccagtg
     2581 gggtgacccg cgatttgcgc aaggatgaac cttacctagc gtactctgat tttgacttcc
     2641 aagtgtgttg cgcccagggc ggggattgtt acgcccgtta tttggtccgc atggccgaga
     2701 tgcgagaaag cctgaagatc atcgagcaag ctgtggagaa cttaccgtct ggtccggttc
     2761 atgtggatgt cggtcagcgg gccattatcc cggacaagtg gcaagttcac tccacgattg
     2821 agggattgat cacgcatttc gagatcatca tgcaaaaccg cggtttcgaa gtgccgtgtg
     2881 aagaagttta ctgtgcgacc gaatcaccca acggggaatt gggtttttac atcgtcggtg
     2941 acggttcgcc ctatgcctat cgggcacggt gtcggccgcc ttcgtttgtc cactttgcac
     3001 tctttccgca tctgattcgg ggccacatgt tgggagatgt tgttgctgtc ctggggagtt
     3061 tgaatattat cgcggcggaa ttggatcggt gaacaagatt gccctggata cggggtgaat
     3121 caatgtcgca agaatcaaag gcacaaacgg ttgaaggtag ggggggcgcc ttgcttcccc
     3181 cggaggtcat tgcgtcaatt cgcgagtatt tcccccgtta tccgaacacg cgtgccgttg
     3241 tcctgccagc ccttcacctg atccaagaga cccttgggta cgtccccaaa agcgccgtgc
     3301 tggagttggc caacgttttg ggaattccag cctcggacat tgaagacacg ctgtcgttct
     3361 accaattctt caaacaggat cggccgctcg gaaagtaccg catttgggta tgtcgttctc
     3421 tgagttgtgc cttgtgtggc ggggaaaacc tgctcgatta tttgcgcgaa cgactcaata
     3481 tcgaacctgg cgaaacaact gcggacgggc gtttcacctt ggaagcggcg gagtgccttg
     3541 gggcctgcga atacgccccg gccgtcctta ttaatgaaac gctctatccc acaatgacgc
     3601 gggagaaact ggaatcagtc ctcg
//
LOCUS       DAOPTR010000313         3613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_20606_length_3613_cov_3.705734, whole genome shotgun sequence.
ACCESSION   DAOPTR010000313 DAOPTR010000000
VERSION     DAOPTR010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20606_length_3613_cov_3.705734"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PKI05_07090"
     CDS             complement(<1..621)
                     /locus_tag="PKI05_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52005.1"
                     /translation="MKLGRREQIMILIVGTVAAIVVGQLIWRLVATPFTARQKALATL
                     TSNVHTRERQLKELEKAKQKLEEWRARSLPADPVLARRLYQNWLLECSQKAQLENIQV
                     DSGEVRRERDLFYRLPFTVRAQGSLESLTRFLHSFYSRNYLHAIQRIAVTPLKDGKKL
                     DLLFTIEAAALDDAVAKDSLPTGELVEAKLDDVKEYTKTIVGRNVFV"
     gene            complement(626..2188)
                     /locus_tag="PKI05_07095"
     CDS             complement(626..2188)
                     /locus_tag="PKI05_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52006.1"
                     /translation="MAYWLAIEWNNEELRAVAAVRRRDRLVIEHAFAVPLTDAVRPSD
                     NGSPESREQTAENIGKRLAAALAARGIRRPTTFVAISRSLVELRQMSVPPVSDEELPD
                     LVRFQALREFTHVNEDWLIDYLLLDEDPSQPRNALVAALAPELFQDIQKTCQIAKVQP
                     ERIVLRPCATASLANRCVPPKPDEIRLLVDLLGEEVDLSVVVGNKLVFMRTARVTGDP
                     LKEADALVVLVGEIRRTMMAAQNQLGGGRANHLVLCGQTPAYQALGQTLRERLDIETT
                     CFDPFSHFDLGPEVAESLPDHPGRFTPLLGVLADEQEKVAPAVDFLHPHRRPEPPSQR
                     NRYALAGLGGLLIVVLAGMYGWLQLQELDSRIAGLRNRQAVLDRQLKQAAETRKLADA
                     IQAWSGGHVNWLEELRRLNEKIPPAEEVMLGKLTMASGARGGEISLEGFAARSESIEK
                     MEQALDDQQHRVISKGKAEDDSQKPYKLRFGTTVVIVPPSSASTSRPSSQRPATGPAA
                     SRTTPNAPLAGR"
     gene            complement(2195..>3613)
                     /locus_tag="PKI05_07100"
     CDS             complement(2195..>3613)
                     /locus_tag="PKI05_07100"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOA52007.1"
                     /translation="AARFFVSMDEEWQNDNGGWFNNPSWFAGVVVQEGADPRETSLFS
                     IIGPDWSDYGASAIRYGLENESAKLNINSLLLADKTVENGGRQLLMALPGMTEDIADA
                     ILDWLDADDEPREFGAELEYYTTLPTPYTPKNGPLETIEELLLVRGVTPELLFGADRN
                     RNGVIDPGETIPEVFANLSADDPVAYRGWSAYLTLFSMELNVRPDGSPKIDINQSDLE
                     KLYDEIEAEFGPELATFIVAYRQNGPYTGEEQGEPVPLNASLDFSRQAKVKFDTVLDL
                     IGAKVRVQFAGEQEPRVLDAVVPEDPVALRVILPVIMANLTATSSKVIPGRININLAP
                     PSILYGIPNLDPGVADLILEARPSDPTFIDDENYYYETWLLTEGLVTLEEMKALMPFV
                     TCGGSVFKAQVVGYFAGGGPSCRVEAIFDATVRPARLLFWRDMSHLGRGFQVDVLGTP
                     SSSYGGAVIPGGSESGATLPGS"
BASE COUNT          803 a         1087 c          972 g          751 t
ORIGIN      
        1 cacaaagacg ttccgtccaa caatcgtttt cgtgtattct ttcacgtcat ccagttttgc
       61 ctcgaccaac tcgccggtgg gaagactatc cttggccacc gcatcgtcga gagccgccgc
      121 ctcgatggtg aagagcagat cgagcttttt gccgtctttt aaaggagtga cggcgatccg
      181 ctgaatggca tggagatagt tccgggaata aaaactatgc aaaaaccgtg tcaacgattc
      241 cagcgatcct tgggcacgga ccgtaaaggg aagccgataa aacaaatccc gttcccgtcg
      301 gacttccccg ctatcgactt gaatgttttc cagttgggcc ttttgggaac attccagcaa
      361 ccagttttga tacaagcggc gggccagcac cgggtcggca ggaagggagc gcgcacgcca
      421 ctcctcgagc ttttgcttgg ccttttccag ttccttgagt tgccgttccc tcgtatggac
      481 gttgctggtg agcgttgcca gggccttttg gcgggctgtg aagggcgtgg ccaccaaacg
      541 ccaaatcagt tgccccacga cgattgccgc cacggtgccc acaatcaaga tcataatctg
      601 ttctcggcgt ccgagtttca tggcgtcacc tcccggcaag tggagcgttc ggagtggtac
      661 gagaggcggc ggggcccgtg gctgggcgct gcgatgaagg ccgcgatgtt gaagccgacg
      721 acggcggaac aatgaccacc gttgtgccaa aacgaagttt ataaggtttt tgggaatcgt
      781 cctcggcttt gcccttggag atcacccggt gctgttgatc atccagggcc tgttccattt
      841 tttcgatgct ttcgctccgg gcagcgaaac cttccaggct gatttcaccc ccgcgtgccc
      901 ccgaagccat cgtcaatttg ccgagcatca cctcctcggc tggtggaatc ttctcattaa
      961 ggcgccgcag ttcttccagc caattgacgt gcccgccgct ccaggcttga atggcgtcgg
     1021 ctaacttgcg ggtttccgcc gcttgtttca actgccgatc gagcaccgcc tgacgatttc
     1081 gcagccctgc aatgcggcta tcgagttcct gaagttgtaa ccagccatac atgccggcca
     1141 agacgacgat caaaaggccc ccaagccccg ccagggcata acgattccgc tgtgacggcg
     1201 gttccggacg ccggtgcggg tgaaggaaat ccaccgcggg agccaccttc tcctgttcgt
     1261 cggccagaac acccaacagc ggggtaaagc gtcctggatg atcgggcaaa ctctcggcta
     1321 cttccgggcc caaatcgaaa tgggaaaatg gatcaaagca ggtcgtctca atatcaagac
     1381 gctctcgtag tgtttggccc aaagcttgat acgccggtgt ttggccgcac aagaccaaat
     1441 ggttggcccg accgccgccc aattgatttt gggcggccat catagtccgc cgaatctcgc
     1501 ccacaaggac caccagcgcg tcggcctcct ttagcggatc gcccgttacc cgcgcagtcc
     1561 gcataaagac caatttgttc cccacaacca cggagagatc gacttcctct cccaaaagat
     1621 cgaccaggag gcgaatctcg tcgggcttcg gcggcacgca gcgattggcc aaactggcgg
     1681 tcgcacaagg acggagaaca atccgctcag gctggacctt ggcgatttgg catgtctttt
     1741 ggatatcctg gaacagctcc ggggcgaggg ctgccaccaa ggcatttcga ggctgcgagg
     1801 ggtcttcgtc aagaaggaga taatcgatca gccagtcttc attaacatgg gtgaactcgc
     1861 ggagggcctg aaatcggacc aaatcaggga gctcttcgtc ggacacggga ggaaccgaca
     1921 tttggcggag ctcgaccagg gaacggctaa tggccacgaa ggtggtcgga cggcgaatgc
     1981 cccgagcagc caaggccgcc gccaagcgtt tgccgatatt ctccgcagtc tgttcccgcg
     2041 attcggggga gccgttgtcg gaggggcgaa cggcgtcggt aagggggacg gcgaaggcgt
     2101 gctcgataac aagacgatcc cgccgccgca ccgccgccac ggcccgcaat tcttcgttat
     2161 tccactcaat agctaaccag taggccatac cttatcaact tcccggtaaa gtcgccccac
     2221 tttccgaccc ccctgggatg accgctccgc cgtaggaact tgagggggtc cccaaaacat
     2281 ctacttgaaa tccccgaccc aaatggctca tatctctcca gaataatagc cgggccggac
     2341 gaacggtagc atcaaaaatg gcctccacgc gacagctagg ccctcccccg gcaaaatagc
     2401 caacgacctg ggctttaaac acactaccgc cgcatgtcac aaagggcatt aaggccttca
     2461 tctcttccaa agtcaccaac ccctccgtga gaagccaggt ttcataataa taattctcat
     2521 catcaataaa cgtgggatcg gaagggcgag cctcaagaat caaatccgca acacccggat
     2581 ctaggttcgg tatcccgtaa aggattgacg gcggtgccaa attgatgttg attcgacctg
     2641 ggatgacctt actcgatgtg gccgtcaaat tggccatgat caccggcaaa atcactcgga
     2701 gcgccaccgg gtcttccggc acgacggcat ccaacacccg cggctcctgc tccccggcaa
     2761 attgcacacg gaccttggcc ccgatgaggt ccagaaccgt atcaaacttg accttagcct
     2821 ggcgggaaaa atcgaggctt gcgttcaagg ggacgggttc gccttgttcc tctccggtat
     2881 agggcccgtt ctggcgatac gccacaataa acgtcgccaa ttccgggcca aattcggcct
     2941 cgatttcgtc gtacagtttc tccaggtcac tctggttgat gtcgatcttg ggagaaccat
     3001 cagggcgaac gttcagttcc atgctgaaaa gcgtcaaata agccgaccac ccacggtacg
     3061 ccacgggatc atccgcgctg agattggcaa agacctcggg gatcgtctct cccggatcga
     3121 tgacgccatt gcggttacgg tcggccccaa acaatagctc cggcgtcaca ccacgtacaa
     3181 gcagcagttc ctcgatggtt tccagtggcc cattcttagg cgtgtaaggg gtgggaagcg
     3241 ttgtgtaata ctccagctcg gccccaaatt cccgcggttc gtcatcggca tcaagccaat
     3301 cgaggatcgc atccgctata tcctccgtca tccccggtaa agccatgagc aactggcgac
     3361 caccattttc cacagttttg tcggccaaaa ggagactgtt gatattcagc ttggccgatt
     3421 cgttctccag gccatagcga atcgccgagg ccccatagtc gctccaatcg ggcccgatga
     3481 ttgaaaatag gctggtttcc cggggatcag ccccttcctg gacaaccact cctgcaaacc
     3541 acgacgggtt gttaaaccaa ccgccgttgt cgttttgcca ttcctcgtcc atggagacaa
     3601 aaaacctagc agc
//
LOCUS       DAOPTR010000314         3595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_20727_length_3595_cov_3.243785, whole genome shotgun sequence.
ACCESSION   DAOPTR010000314 DAOPTR010000000
VERSION     DAOPTR010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20727_length_3595_cov_3.243785"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2218
                     /gene="fliD"
                     /locus_tag="PKI05_07105"
     CDS             <1..2218
                     /gene="fliD"
                     /locus_tag="PKI05_07105"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOA52008.1"
                     /translation="AINRNMRINVLAEVVGDHIRLTDRTGMTASNLRVQDLGNGRAAA
                     SLGLAGIDVAESTADGSDIVWLTTDTLLSDLNDGLGISASSTLPDISFQLRDGTTGTI
                     DFDPKPENGEERQTEKTLGDIIARINAAAPGKLQAELSADGKRLVLRDLTEGTNEFKL
                     EALYGSQALHDLGLDVAAEGDTITGQRLIGSLKSPALSQLNGGQGIGTLGLIRVTDRM
                     GQQAVVDLSTADTLEDVIRAINAAEIGVTASVNSARNGIVIQDTSGGTGHLIIESLDD
                     NQSAEKLHIAVNDAVSSKDSGDLHLRVISVNSRLADWNGGQGVSLGRFTVTDSKGVTG
                     TIDLAAIQAQTVGDVIAAINRTAANVIASINETGDGILIRDLANGSGKLVINDLGGTT
                     AKDLGLLRESTLRDFNGDSDYVIDGSLTYRIELDKNDTLTTLTEKINGLKAGVKASIL
                     YDGSTRPYRLSLVGERGGNVAQLVLDDSQLGIDLQRITQARDAQLALGYVGSAGAVVL
                     SSRTSTFSGIVPGLELDVLQGTNTPVNVQVQTSDADLVASVQTFVDNFNKFWKRYKEL
                     TKYDTETQKGEVLASDPTAIRLGSEVSYLLSSRFAGAGKIQSLAEIGISINQDGSLSF
                     DSSVLQAKYAEDPEAVKKFFTDATFGFAKKYDKLSESLAGENVSLISQRLNALSQKYE
                     DNSEKIKWWNEKLERRRQQLLLYFYRLENTIAKLKANLDTLSALQPISYDWLRKSK"
     gene            2294..2839
                     /locus_tag="PKI05_07110"
     CDS             2294..2839
                     /locus_tag="PKI05_07110"
                     /inference="COORDINATES: protein motif:HMM:NF014604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HOA52009.1"
                     /translation="MSASVGDSYREGEILTAPPQRLHLMLIEGAIRYTERTRHFWREN
                     QNDAAFESLLRAQQIVSEILAGIKISDEFPLARKVAAIYTFIYRALVQAGLTREEKYL
                     DDALRVLHIQRETWEEVCRRLGGSSPSTDDIRISEAAASVAAKETIRSAPAAPHASPP
                     LTNLRTPDYPQEYATGFSWEA"
     gene            2877..>3595
                     /locus_tag="PKI05_07115"
     CDS             2877..>3595
                     /locus_tag="PKI05_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA52010.1"
                     /translation="MPKDVTLIRVGHSPDPDDAFMFFALAKEKIPTGRYRFTHELADI
                     ETLNRRALQGELELTAISFHAYAFVRKHYILCHCGASMGDGYGPIVVARSSMNVNDLR
                     QTPIAVPGRWTSAFLALRIFLQDEFPFVIVPFDEIPRAVASGSYQGQPIGAGLLIHEG
                     QLTFGQQGLKLIVDLGAWWKEQTGLPLPLGGNAIRRDLGQQVIADVHHLLRESIDYAL
                     SHRDEALAYALNFARDLDRPLA"
BASE COUNT          797 a         1030 c         1034 g          734 t
ORIGIN      
        1 ggccatcaat aggaacatgc ggatcaacgt cttggccgag gtggtgggcg accatattcg
       61 cctgaccgac cgcacgggga tgacggcctc caatctccgc gtgcaggact tgggcaatgg
      121 gcgagcggcg gcctccttgg gtctggcggg gatcgacgtg gccgaatcca ctgccgacgg
      181 aagcgacatc gtttggctaa ccaccgacac actcttgagc gacctcaacg acggactggg
      241 tatttccgcc agttcaacgt tgcccgacat ttccttccaa ttgcgagatg ggacgaccgg
      301 gacgatcgac tttgatccta agccagaaaa tggtgaagag cgacaaacag agaagacgct
      361 gggagatatc atcgctcgca tcaatgcggc ggccccgggt aagcttcaag cggagctgtc
      421 ggccgacgga aaacgacttg tcctccgcga tcttacagaa ggaactaatg agttcaaatt
      481 ggaggccctc tacggttctc aggcccttca tgacctgggg cttgatgtgg cggccgaggg
      541 cgacaccatc accggtcagc ggctgatcgg ttcgctcaaa tcaccggccc tttcccaact
      601 caacggcggt cagggaatag gaacgttggg actcatccgt gtgaccgacc gcatgggaca
      661 acaggccgtg gtcgatctct ccactgccga caccctggaa gatgtcatcc gggcgattaa
      721 cgcggcagag atcggcgtca cagccagcgt gaattcggcc cgcaatggca tcgtgatcca
      781 ggataccagc ggcgggacgg gtcatctgat catcgagagc cttgacgaca accaatcggc
      841 agagaagttg cacatcgctg tgaacgatgc ggtatcctcc aaggacagcg gcgaccttca
      901 cctgcgagtg atttccgtca attcccgcct ggccgattgg aacggtgggc aaggcgtatc
      961 cctcgggcgt tttaccgtca ccgacagcaa gggtgttacc gggacgatcg atctggcagc
     1021 gattcaggcc cagacggtgg gcgacgtgat cgcggccatc aatcgtacag cggccaacgt
     1081 catcgccagc attaacgaga cgggcgacgg catcctcatt cgggatttgg ccaacggttc
     1141 gggaaagctg gtgattaacg acctgggagg aaccacggcc aaagacttgg ggctacttcg
     1201 cgaaagcaca ctacgcgact tcaacggtga cagcgattac gtcattgacg gttccctgac
     1261 ctaccgcatt gaattggaca aaaacgatac actgaccacc cttacggaga agatcaacgg
     1321 cctcaaggct ggtgtcaagg cgagtatcct ctacgatggg tcaacccggc cgtaccgcct
     1381 gtccttagtc ggcgagcgcg gggggaatgt ggcccaactc gtcctcgatg attcgcagtt
     1441 ggggatcgac ctccagcgga tcacccaggc gcgggacgca caattggccc tgggatacgt
     1501 tgggtccgcc ggggcggtgg tgctttcctc acgaacgagc accttctcgg ggattgtgcc
     1561 gggattggag ctggacgtgc tccaggggac gaacacaccg gtcaatgtcc aggttcaaac
     1621 gagtgatgcc gatctcgtgg cgagtgtgca gacattcgtc gataatttca acaagttttg
     1681 gaagcgctac aaggaactga ccaaatacga cacggagacg caaaaaggcg aggttctggc
     1741 ctcggatccc acggccatcc ggctaggcag cgaggtctcg tacctcctca gttcgcgttt
     1801 tgctggggcg gggaagattc agtcgctggc ggagattggc atttcgatca atcaagatgg
     1861 gtccctcagc tttgatagct cggtcttgca ggcaaaatac gctgaggatc cggaggccgt
     1921 taaaaagttt tttacagatg ccacgtttgg ctttgccaaa aaatatgaca aattgagcga
     1981 gagcctggca ggtgaaaatg tatcgctcat ttctcagcgg ctcaacgccc tttcccaaaa
     2041 gtacgaagat aactctgaga agattaaatg gtggaatgag aagctagaac gacgcaggca
     2101 gcagcttttg ctgtatttct atcggttgga gaacacgatc gccaagttga aggcgaattt
     2161 ggacacgttg tcggcgctcc agcccatttc ctatgattgg ctccgcaaat ccaagtgaga
     2221 ttgatggagc cacttagacc caacatcaat cccgcatgat aaccttggag atttgccgga
     2281 agggactctg cctatgtctg cttcagtggg tgatagctac cgcgaaggcg agattctcac
     2341 ggcgccacct cagcggctgc atctgatgct catcgaaggt gccatccgtt ataccgaacg
     2401 cacgaggcac ttctggcgag agaaccaaaa tgacgcggcc tttgagtcgc tccttcgtgc
     2461 ccagcaaatc gtcagcgaaa tcctcgcagg catcaagatt tcggatgagt ttcccctcgc
     2521 acggaaagtt gcggccattt acaccttcat ctatcgcgcg ctcgttcagg ccggtttgac
     2581 ccgcgaagag aagtacctcg acgacgccct ccgggtcctt cacatccagc gtgaaacgtg
     2641 ggaagaggtc tgccgtcgcc ttggtgggtc ctcgccttca acggatgata tccggatcag
     2701 cgaggccgcg gcttctgtag cggcaaagga aacgatccgc agcgccccgg ccgcccctca
     2761 cgccagcccg cccttgacaa acctccgcac ccccgattat ccccaggaat atgccacagg
     2821 gttttcctgg gaggcctagc aggccacacc ctcttgccgc gtgagaggat ttcctgatgc
     2881 cgaaagatgt cacactgatt cgggtaggac atagtcccga tccggatgac gccttcatgt
     2941 ttttcgccct agcgaaggag aagattccga cagggcgcta tcgttttacc cacgagttgg
     3001 ctgatatcga gacgcttaat cgccgggcgc tccaaggcga actggaattg accgccatca
     3061 gcttccatgc ctacgccttt gtccgcaagc attacatcct ttgccactgc ggggcaagca
     3121 tgggggatgg ctacggcccc atcgtagtag cccgatcgtc gatgaacgtg aatgatctgc
     3181 ggcaaacccc gatcgccgtt cccggacgct ggaccagtgc cttcttggcc cttcgaatct
     3241 tcctgcaaga tgagtttccc tttgtgatcg tcccctttga tgagattccc cgcgccgtgg
     3301 caagcggtag ctatcaggga caacccatcg gtgctggact gctaatccac gaaggccaac
     3361 tgacctttgg ccagcagggg ctgaagctga tcgtcgatct cggggcgtgg tggaaggaac
     3421 aaaccggact gccgttgccg ctcggcggaa atgcgatccg ccgcgacctt ggacagcagg
     3481 tgatcgccga tgtccatcac ctgctccgcg aaagcatcga ctacgcgttg tcccaccgcg
     3541 acgaggccct tgcttatgcc ctaaattttg ctcgtgactt ggacaggcct cttgc
//
LOCUS       DAOPTR010000315         3593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_20742_length_3593_cov_3.200961, whole genome shotgun sequence.
ACCESSION   DAOPTR010000315 DAOPTR010000000
VERSION     DAOPTR010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20742_length_3593_cov_3.200961"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PKI05_07120"
     CDS             <1..251
                     /locus_tag="PKI05_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52011.1"
                     /translation="LVFGIGLAFLIVGVLVFISSVFYVAIAGLIIGSAVAGWIGSSAR
                     RSQVADRFNLDGAPAGPQAPADRRTETTPSSDDGIDIG"
     gene            549..1592
                     /locus_tag="PKI05_07125"
     CDS             549..1592
                     /locus_tag="PKI05_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52012.1"
                     /translation="MGTGLKRATKSIPATVGQGLPSHWRTLYICDRSRTGAWLVESFA
                     AESPAEVEVHEAPGRTAGLARLRDEIFDVIFIVHDPPELDAIDLTVAYRTGGTDEALI
                     VLGRQSEQELAVPCYEAGADGYVCVSTATSHVLKWTALRAVQRCHLTRQNRRFQQSEL
                     QRLQREREEALRVLLHQKHLISDSSSEPSTRCCGPLRTTSQRPSDDSSCDHLSQFELP
                     EPPDLPAKLIAHYRELLRAYIIMGSGHLGIELRELADVFATAGLSPKEVLHLHLYVAA
                     ELIRGLGSRSTRHVITRTNLLIMEVLLQLAEAYRSRYRDASRPPIQLFFPQFEQCMRD
                     ITASRPTIPDESC"
     gene            complement(1624..2598)
                     /locus_tag="PKI05_07130"
     CDS             complement(1624..2598)
                     /locus_tag="PKI05_07130"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOA52013.1"
                     /translation="MLVRLFVVRGKANKGAVVIRLPAVIGRSRDADLTIVHPMISRRH
                     CQLFESEGLVKVRDLGSLNGTYLGGEQIQEAFLPPGSVLTIGPLVFRVEYEVSPAAIS
                     REPSVVDSHPAAPSDEMLVMEESHTVGSDSRQTGGFPTPVQAGVSGGSAEPATEPLPS
                     HFPETPVVIHESADGREPLEGGIAPPDGAMPDARAWQQVIQGDGDHSEESSEEAASGE
                     KTERNCSINTHGPYQGETRQGDQKPKGEKTPWADAPPPFTVVQKPADNASSPILLDSG
                     ADTNDDSSRRGGPAGSVFQSLGRLSSSQNVSSHTAQGARPRGWWFFGR"
     gene            3300..>3593
                     /locus_tag="PKI05_07135"
     CDS             3300..>3593
                     /locus_tag="PKI05_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52014.1"
                     /translation="MNSFASRRINHCVRLRALCAVFAGVVIASFGWQNDVSGEEAGRW
                     HVGKPIVTYWAGPPLTEAVAAQLAEGGWNVVWGTEKDLETAAKHGLRLQLQDPL"
BASE COUNT          759 a         1074 c         1007 g          753 t
ORIGIN      
        1 acctggtttt tgggatcggg ttggcctttt tgatcgtcgg cgtcctggtc ttcataagct
       61 cagttttcta cgtcgccata gcgggattga ttattggcag cgcggtggcg ggttggattg
      121 gtagctccgc tcgccgttca caagtggcag accgcttcaa ccttgatggt gcccccgccg
      181 gcccgcaagc tccagcggac cgtcgtaccg agaccacgcc atcgagcgac gacggtattg
      241 atattggcta gatagccgcg agaaggtgcg gcctcggtcc gcctctcgat ggaagccgcc
      301 caaaatggga atccgtttgc gccctaaaac gaccttagcg ttttgcgtca cggcgataac
      361 tggcttggcg tagcgtgaag agttgaaaag ctcgtcaccc ccccaaacag cctatgtcca
      421 agcttcacat tcgtgacgta gcaaaccggt cttgacgtga aggaaacaat tcggctactc
      481 tcaggacgcc ttgtaggaat ggggtattgg gtatcgtatt ttgcaaccca ccagtgaaac
      541 gccaaccgat gggcaccgga ctcaaacgag cgacgaaatc aatacccgca accgtgggac
      601 aggggctgcc ttcccactgg cggacgctct acatttgcga tcgctcccgc acgggcgcct
      661 ggctggtgga aagcttcgct gcggagtccc ccgcagaagt tgaggtccac gaagcgccag
      721 ggcgaacggc aggcctggcc cgtcttcgcg atgagatctt cgacgtaatt ttcatcgtcc
      781 atgatccccc tgagctggac gcgatcgacc tgaccgtcgc ttatcggaca ggcggaaccg
      841 acgaggccct gatcgtcctt gggcggcaaa gtgagcaaga attggccgtc ccctgttacg
      901 aagccggggc tgacggctat gtctgcgtca gtacagcaac cagccacgtc cttaagtgga
      961 cggcactgcg ggcagtccaa cgttgtcatc tcactcgcca aaatcgtcga ttccagcaaa
     1021 gtgaattgca acgtctgcag cgcgagcgtg aagaggccct acgggtcctc cttcatcaaa
     1081 aacatttaat ctccgactca tcctccgagc catcgacgcg gtgttgcggg ccccttcgaa
     1141 ccacgtccca gcggccgtca gatgattcgt cttgcgatca tctctcccaa tttgagttgc
     1201 cagagcctcc cgacttgccc gccaaattga tcgcccacta tagggaactt ttgcgggcat
     1261 acatcatcat gggttcagga catctcggca tcgaactgcg ggaactggcc gatgtctttg
     1321 caacagcggg gctgtccccc aaggaagtgc tccaccttca tctctatgtg gctgccgaat
     1381 tgatccgagg gcttgggtcg cggagcaccc gccatgtgat cacccggacg aacctcctga
     1441 ttatggaagt cctcctccag ttggcagagg cctaccggtc acgttatcgg gacgcctcgc
     1501 gaccgcccat acaactcttc tttccgcaat tcgagcagtg catgcgagac attacggcat
     1561 cacggccaac catccccgat gagtcctgct agcgccaggt cgcacgcgcc accaacgtcc
     1621 aaactaacgt ccaaagaacc accatccccg cggcctagcg ccttgcgccg tgtggctgga
     1681 aacgttttga gaagatgaca acctgccgag ggactgaaag acacttcccg ccggaccacc
     1741 gcgtcgggac gagtcatcgt ttgtgtctgc tcctgaatct aacaagatcg gcgaggaagc
     1801 gttgtctgcc ggcttttgga caaccgtgaa cgggggtggc gcatccgccc agggtgtctt
     1861 ctcacccttc ggcttctggt ccccttgcct cgtctccccc tgatacgggc catgcgtgtt
     1921 gatcgagcaa ttccgctcgg ttttctcgcc tgaggccgct tcctccgatg attcctcact
     1981 gtgatcgcca tcaccctgga tcacttgttg ccacgccctc gcatctggca tggcaccatc
     2041 gggtggagcg attccccctt cgagtggctc acgcccatcg gctgactcgt ggataacgac
     2101 cggcgtctct ggaaaatggc tgggaagagg ctccgtggcc ggttcggcac tcccgccgga
     2161 gacccccgct tggaccggcg tggggaaccc acccgtttgt cgggaatcac ttcccaccgt
     2221 atgggattcc tccatgacaa gcatctcgtc ggagggcgca gccggatggc tatcaaccac
     2281 ggacggttct cggctgatcg ctgccgggga aacttcgtac tccacgcgaa agacgagtgg
     2341 tccaatggtc aggacactcc ccggggggag aaacgcctcc tgaatctgct caccacccaa
     2401 ataagtacca ttcaaagagc ccaaatcccg aactttcacc aggccttcgg attcaaagag
     2461 ttggcaatgg cggcggctga tcatggggtg aacgatcgtc aaatcggcat cgcggctgcg
     2521 accgatcacc gctggtaacc ttatgaccac cgcaccctta ttggctttgc cgcggacaac
     2581 gaaaagccga acaagcattg gggaggcttc tctcgtttat tggggacgtc gctgggcagg
     2641 taattaaagg caaccgaatt tattctaccc accgccgtca aatccctcaa tgggattttt
     2701 ccgccgccat cccgtttctc aggacacaat ccctacggca aaagccagag aagcaagtaa
     2761 cttgctggga gtaggcactt agagcctatc ccaaagactg cctctccctc cgggagaggg
     2821 ttggggtgag ggcaagcgaa cgtcggagtc gatcgagaac accggcagcg attcgagcca
     2881 ccctcacccc aggcccctct ccggctgaac ccacgagggg agattatggg ataggcaatt
     2941 gggttgacaa ggcggcgtgg agaggtgcgc acctcaggcc aaggcccaca tcccaggaaa
     3001 ttttacctgg caagaaaatg ccgccggcgg agtccttggt cctatagcat gggctggaag
     3061 accatgccgc tgactgtctt gccgttaggc cctagcgtct gaatcaagtc tcacaaggaa
     3121 gtgacaagca cataactaag ttcttacgaa tcagtgcatc ttgacgcacc tttgagtggg
     3181 gacggatttg gtttatgact gtgcgatgct tgacgcgatc atgggggagc gttacacata
     3241 ggccaaccgg acgtaactct tttccaaaaa catgcccgtt ttctatgtga ggcactacta
     3301 tgaacagttt tgcaagccgc agaatcaatc attgcgtccg ccttagggcg ctatgtgcag
     3361 tattcgccgg tgtggttatc gcctcttttg gatggcaaaa cgacgtttcc ggggaagagg
     3421 caggccggtg gcacgtcggc aaaccgatcg tcacctactg ggcaggtccc ccgttgaccg
     3481 aggccgtggc ggcacagctt gccgaaggtg gttggaatgt tgtctggggc acggaaaagg
     3541 acttggagac ggcggccaag cacggactac gcttgcaact tcaggacccg ctt
//
LOCUS       DAOPTR010000316         3590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_20768_length_3590_cov_4.358982, whole genome shotgun sequence.
ACCESSION   DAOPTR010000316 DAOPTR010000000
VERSION     DAOPTR010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20768_length_3590_cov_4.358982"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1922)
                     /locus_tag="PKI05_07140"
     CDS             complement(48..1922)
                     /locus_tag="PKI05_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOA52015.1"
                     /translation="MSLRWLAGGTLLVVFCLGFVWAFWFPHEPPADLTFCNLTEIKTI
                     DPALVTGQPEGRVVWALFEGLTTYDPKTLAPQPGVAESWTISQDKRTYTFHLRKEARW
                     SDGKPLTAEDFVWSFRRLLHPETASEYAYELWHVVNAEKYTTGRVTPGDPVEIELLEK
                     PPGAPPFASGILLRGRLVGIEPANSALGGQTTGEPVYLVEIDGRIERFQKPSREGLPP
                     PDTAARPYRWILYDFEHVGIKALDRHTLQMTLTHPVPYFLEIIGFFPMMPVPRHCIEQ
                     YGYPNWTKPENLVCNGPFILKYRRIRDRIRLVKNLHYWDRENVHLNIVDVLAAESAIT
                     GLNLYMTGAADWIPVVPNEVVSELLRQKRPDFQPSPYLAVEYYVVNTQRPPLNDPRVR
                     RALALAINKRELIDRILQTGQQPALSFVPPSISDYIAYQPPQMPDYDPETARQLLASA
                     GYPNGRNFPKLEILYNTLESHQAIAELIQAQWKRTLGIDVRLQNQDWARYLASRRQGE
                     FWIARAGWIADYLDPNTFLEMMTTDNPNNHGRWSQPEYDRLVALAQVEPDETRRFAYF
                     RQAEEILLQEMPVLPLYFAVTRAMVRPYVRGYYHNVLDIHPLKGMSIDHAQKASLLGR
                     "
     gene            complement(2016..2636)
                     /gene="rdgB"
                     /locus_tag="PKI05_07145"
     CDS             complement(2016..2636)
                     /gene="rdgB"
                     /locus_tag="PKI05_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOA52016.1"
                     /translation="MEKPIGVIGTGNHAKLIELADLLTPAGVTWKFLKDFPHVGEVEE
                     TGCTFSENARLKAVTYAVRLGEWVLAEDAGLVVDALGGQPGIHSARFAGEPRDDARNI
                     QKLLELMKDIPPERRSARFVCYMALADPRGEIQAEAEGHCCGRITFEPRGTQGFGYDP
                     VFEIPEYHRTLAELGLAVKGCLSHRARAARAILPQIIRLVDSGKWR"
     gene            2978..>3590
                     /locus_tag="PKI05_07150"
     CDS             2978..>3590
                     /locus_tag="PKI05_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52017.1"
                     /translation="MLRTLCWVMVVGVTMGTASLLAQDAILNHFYGQGVHKFFARDYV
                     GAFEDFNTAIENGSDDPRCFYYRGLTYLHLGREEEAKSDFEKGAQLEMQAARKFFDVS
                     KALERIQGRQRVMIEQYRTKARLAVMVELERQRKARYEELRREEARVLDRAPPAVGIP
                     EAAPAPPSAPPAAPPAAPSEPAVAEQPPAAQPPAPAAPPAAPPA"
BASE COUNT          804 a          977 c         1011 g          798 t
ORIGIN      
        1 gcatccacaa ttgccgcgtt ggatgaaacc acggaatggg cttcgcctca tcggcctagc
       61 aggctcgctt tttgagcgtg atcgatactc atccccttca aaggatgaat gtccaacacg
      121 ttgtggtaat acccgcggac ataggggcgg accatcgccc gcgtcacggc aaaatagagc
      181 ggtaggacgg gcatttcctg gagaaggatt tcttcagcct gcctgaaata tgcaaaccgc
      241 cgcgtttcat ctggttcgac ttgggccagg gccacaaggc gatcatattc aggctggctc
      301 cagcggccgt gattgttagg gttatctgtg gtcatcatct ccaagaaggt attgggatcg
      361 agatagtcgg cgatccaacc tgctcgcgcg atccaaaact ccccctggcg tcggctggcc
      421 aggtacctgg cccagtcctg gttttgcaat cggacgtcga tccccagcgt cctcttccat
      481 tgtgcttgaa taagttcggc aatcgcttga tgcgactcaa gcgtattgta gaggatttct
      541 agttttggaa agttacgccc gttcggatag ccggccgaag caagcaactg gcgagcggtc
      601 tcgggatcgt aatctggcat ttgcgggggc tgataagcga tgtaatcgct gattgagggc
      661 ggaacgaaac tcaacgcagg ttgttgaccc gtttgcaaaa ttcggtcaat cagttcccgc
      721 ttgttaatgg ccagggccag tgcccgccga acgcgcgggt cgtttagcgg cggtcgctgt
      781 gtgttgacaa catagtattc cacagccaaa tagggtgacg gttgaaaatc gggtcgcttc
      841 tggcgaagga gttcactcac cacttcattg ggaaccacgg gaatccaatc agccgctccg
      901 gtcatgtaga gattgaggcc tgtaatggcc gactcggctg ctaggacatc cacaatgtta
      961 aggtgaacgt tctcacgatc ccagtaatgg agattcttta cgaggcgaat tcgatcacga
     1021 attcggcggt acttgaggat gaacggaccg ttgcaaacga ggttttccgg cttcgtccaa
     1081 ttgggataac catattgttc gatgcaatgc cgggggaccg gcatcatcgg gaagaagccg
     1141 atgatctcca aaaaatacgg taccggatgc gtcagcgtca tttgaagggt gtgacgatcc
     1201 aaggccttaa ttcctacatg ctcaaagtca tagagaatcc accgatacgg gcgggcagcg
     1261 gtgtcaggag ggggaaggcc ttcgcgagat ggtttttgaa aacgttcgat tcgcccatcg
     1321 atttcgacca gataaacggg ctcacctgtt gtctggccgc ccagggcaga atttgccggt
     1381 tcaatgccga ccaaccgtcc ccgcaacaaa atccctgagg cgaacggcgg agcgccggga
     1441 ggtttttcca gaagttcgat ctccacagga tcgcccgggg tgacgcggcc tgtggtgtac
     1501 ttttccgcgt taacaacatg ccacaattca taggcgtact cggaggccgt ttcgggatgg
     1561 agcagtcggc gaaaagacca gacaaagtcc tcggccgtga gaggctttcc atcagaccag
     1621 cgcgcctctt tgcggaggtg aaacgtataa gtcctcttgt cctgcgaaat cgtccaactc
     1681 tccgcgacgc ccggttgagg ggctagcgtt tttggatcat aggtcgttag tccctcaaaa
     1741 agtgcccaga ccacgcgacc ttccggttgg ccggttacca aggccggatc aatcgttttg
     1801 atttccgtaa ggttgcagaa cgtcagatcg gccggtggct cgtgcggaaa ccaaaaggcc
     1861 cacacgaacc ccaggcaaaa gacaactagt agtgtgcctc cggccagcca acgaagcgac
     1921 attccaccag ccttttctaa ggacccaaac aacgatgcaa tgagagacta aagtacggac
     1981 ccaatgttag ctcgaaacct gtgaaaaacg tgccattatc tccattttcc agaatcgacc
     2041 aatcggataa tttgcgggag aattgccctg gcggctcgcg cccgatgact gagacacccc
     2101 tttaccgcca gccccaattc tgccagtgtg cggtgatact caggtatttc aaaaacgggg
     2161 tcgtagccaa atccctgagt tccgcgcggt tcgaatgtga tccggccgca acagtgtcct
     2221 tcggcctcgg cctgaatctc cccccgcgga tcggccaggg ccatgtagca aacgaaccgg
     2281 gcggaccgcc tttccggggg aatgtctttc atgagttcca gcaacttttg gatattgcgg
     2341 gcatcatcgc ggggttcgcc ggcaaagcgg gcggaatgaa tccccggttg gccaccgagg
     2401 gcgtccacga ccaggcctgc gtcctccgcc aaaacccact ctcccagacg cacggcgtac
     2461 gtcaccgctt taagccgggc attttcggaa aatgtacacc cagtttcttc aacctcgcct
     2521 acatggggaa aatccttcag gaacttccag gtcactccag ccggggtgag caaatcagct
     2581 agttcgatca gtttggcatg gtttccggtt ccgatgaccc ctattggttt ttccatgcgt
     2641 ggttccttgt cacagccatc cgttttttgg taccgggcca cttgcaattc ccgcgaagtc
     2701 taattatttt aatcgcattc cctgttttgc taaattaacg cggcctatgg catcagcttg
     2761 aaattgccgg ttttcccaat tagtatgggc aagagtaaag gttaaggtag gtcttttccg
     2821 tgccaacgta acaggctggc tcaatggtgc gcggagtgcc cataaccggc ctgtggcagg
     2881 aaagagaata aacgatacgg cggaggctgt cgcacacgga ggttcgattg cacttgggtc
     2941 acgcaaaagg ttatgacagt cgctgggagc tgactccatg ttgcggacac tttgctgggt
     3001 aatggttgtc ggcgtcacta tgggaactgc ctcgctcttg gcacaggacg ccattctcaa
     3061 ccacttttac ggtcagggcg ttcataaatt tttcgcccgc gattatgtag gcgccttcga
     3121 ggatttcaac acagcaattg agaatgggag cgatgatccg cggtgtttct attaccgggg
     3181 tttgacgtat ctccatcttg gacgggagga ggaagccaag agtgactttg aaaaaggcgc
     3241 tcagttggag atgcaggcag caaggaagtt cttcgatgtc agcaaggccc tggagcggat
     3301 tcaagggcgt caacgagtga tgatcgaaca ataccgcaca aaggcgcggt tggccgttat
     3361 ggtggaattg gaacgtcagc gcaaggcccg gtatgaggag cttcgccgtg aagaggcccg
     3421 cgttttagat cgggcgccac cggctgtggg cattccggaa gctgctccag ccccgccgtc
     3481 tgccccaccg gccgctcccc ccgcagcacc atctgaacca gcggtagcgg aacaaccgcc
     3541 tgcggctcaa ccgccggctc ctgcggcgcc acctgctgca ccaccggccg
//
LOCUS       DAOPTR010000317         3585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_20798_length_3585_cov_3.977337, whole genome shotgun sequence.
ACCESSION   DAOPTR010000317 DAOPTR010000000
VERSION     DAOPTR010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20798_length_3585_cov_3.977337"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PKI05_07155"
     CDS             <1..490
                     /locus_tag="PKI05_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HOA52018.1"
                     /translation="EVEASTIEPYTNKQHHGFIGHSYLGSWVNLGAGTCNSDLKNTYG
                     LVTMEYNGQRVPTGMQFMGCIIGDYTKTAVNTGIFTGKVIGVCCMVYGFVTTNVPSFT
                     NYARLFGQMTEVPVEVAIAGQQRMFLRRNVVQRPCDIQLIRDMYELTRHERRLGNEPL
                     VL"
     gene            complement(557..1837)
                     /locus_tag="PKI05_07160"
     CDS             complement(557..1837)
                     /locus_tag="PKI05_07160"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOA52019.1"
                     /translation="MVRNSSLGADSKEIPVHAGAGEPLLQLSPPNGLSPFRRIGIVGA
                     GLMGSSIAAWHLAKGCQVVITDKSPAALETVRQRVADELTRSGAVDKATCETRLADLQ
                     ISDRLDDLATCDCVIESIVEDANAKTQLFRQLESQLGPETPILSNTSTIPIGKLAMGL
                     KHPERVCGLHFLHPVRSRDLVEVIPHSATHRDIGKRVVEHALAVGKTPVITADIPGFV
                     VNRLLFPYLNEGLQLIAEGFLPNEVDQAAAEIGFLIGPIRIMDEIGLDTTWAAGRVLW
                     EAFPERISPSPILVTMLKHNRLGRKNNRGFFDYSSFSASEVEPSLDPETHKLIAPWMA
                     RPPLPQKLPVGMRLVLAMAFEAALILADNVADDPRVVDACSVLGLGFPRPLGGPCFWM
                     QSVGFSQLKEIVEFTCPSAIAPRVLKVLEEVLRE"
     gene            complement(2214..>3585)
                     /locus_tag="PKI05_07165"
     CDS             complement(2214..>3585)
                     /locus_tag="PKI05_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOA52020.1"
                     /translation="VGFVSGLCAVVVAGWVVLGTFGVHTAAAQVQPGSPPATTIPGLP
                     GIPGLPGTTPQKPEEKPVYPTVPSYLELSADDSLAKQAANVQRMLKEGNFTAVANGQD
                     VFRDYYEKYFFPSWTKATNRSKLQQLRSQLIVTELGNYARAGGPPRDLLVQIAFNTLS
                     GYVKNDELDPAVRVAALLAIGDLNERERPTGGGMQPPVPLASALPFLVSILKDPQQLD
                     ALRLAALVGIERHCGAGIADTNFRDSEVIPLLVQLASDKNVPEGKDPKVHEWLRVRAI
                     DVIAITGQAGPNGQNIQMLLSFVTDDKESPKVRLAAARALGSFDYSQVQGVNLLTIPQ
                     GLGKMVLDLCVADLQTVSQTGNWTKLETRLLAQYVSSAQQGLRGVRAAFAGKPEDQLI
                     SKLNRSLDEVTKALNDSLNARNPTMELVRLLPTALSNLNTALQGFAAADSGAPSANEG
                     AQAGSP"
BASE COUNT          836 a         1029 c          975 g          745 t
ORIGIN      
        1 agaggtcgag gcctccacca tcgagcccta caccaacaag cagcaccatg gttttattgg
       61 ccacagctat ctggggagct gggtcaacct aggggcagga acctgcaata gtgaccttaa
      121 gaatacctac gggctggtga caatggagta caacggccaa cgtgtcccca ccggaatgca
      181 gttcatgggg tgtattatcg gcgattacac gaaaacagcg gtcaacacgg gcatcttcac
      241 gggcaaagtc atcggcgttt gttgcatggt ttatggcttt gtcacaacta atgtccccag
      301 tttcaccaat tacgcccgac tctttggtca gatgaccgag gtccccgtgg aagtcgccat
      361 tgccggtcaa cagcggatgt tcctccgtcg caatgtcgtt caacggccat gcgacatcca
      421 gcttatccgc gatatgtatg aactgacccg tcacgaacgc cgattgggca acgaaccgct
      481 cgtcttgtga agaacaaaga tgagtcccca ccttgggccc gggaaagaac cttcgccatc
      541 tagcgggcgc gtgatactac tcccgcagca cttcttcaag gactttcagc actcgcggag
      601 ctattgccga gggacatgtg aattccacaa tctccttcaa ctgagaaaaa ccgacggatt
      661 gcatccagaa gcacggcccg cccaggggtc gcggaaagcc taacccaagg acggaacagg
      721 cgtcaacgac gcgcggatcg tcggcgacgt tgtcagccaa aatgagggcc gcctcaaagg
      781 ccattgccag gacaaggcgc atgcccacgg gtaacttctg cggtaatggt ggacgggcca
      841 tccatggggc aatgagtttg tgagtttctg gatcaagtga tggctcgact tcgctcgcgg
      901 aaaaggacga atagtcgaag aacccgcggt tattttttcg gcccaatcgg ttgtgcttca
      961 gcatggtgac gagaattggt gaagggctga tcctctccgg aaatgcctcc caaaggactc
     1021 gaccggcagc ccaggtggta tccaaaccga tttcgtccat aatccgaata ggcccgatca
     1081 aaaagccaat ttccgcagcc gcctggtcaa cctcattagg aaggaacccc tcggctatca
     1141 gttgtaggcc ttcgttaagg tagggaaaaa gaagacggtt gaccacgaag cccgggatat
     1201 ctgctgtgat aacgggagtt ttaccaacag ccagagcgtg ctccacaacc cgtttgccaa
     1261 tgtccctgtg tgtggccgaa tgggggatga cctcaacgag gtcccttgaa cggaccgggt
     1321 gcaagaaatg aagtccgcag acacgttctg ggtgtttcag ccccattgcc agtttgccga
     1381 tgggaatggt ggaggtattc gacaggatcg gcgtctctgg cccgagttgc gactcaagct
     1441 gcctgaatag ttgcgtcttg gcgtttgcgt cctcgacgat ggattcgatg acacaatcgc
     1501 acgtggccag gtcatccaag cggtcagaaa tctgaaggtc ggcaagccgg gtttcacagg
     1561 ttgctttatc gaccgccccc gatctcgtca actcatcagc cacccgttgc ctgacggttt
     1621 ccaaggccgc tggagacttg tcggtgatca ccacttggca tccctttgca aggtgccacg
     1681 cggcaatgct gctgcccatc agaccggcgc caacgatgcc aattcgtcga aagggcgaga
     1741 gaccattcgg cggcgacaat tgcagaagag gttccccggc acccgcgtgg acagggattt
     1801 ccttgctatc ggccccaaga cttgaattac gcaccatgtc cagcgccttg gcacggcacc
     1861 aggctgccaa cttctcgcaa taagctaaac aatcttccgc attgggtttg ggaatatcca
     1921 tctcgtggga ttgtctagaa actgggccga aggatgcctc gcccctccaa ccaggtgaaa
     1981 gcctcccacc atggtccgtc cacgcaggaa ctctcacagc atttcgcaga tacaggctat
     2041 tctatcagag gaaggtccct cggaatcagc tcggcgcaac caagtcgcgg tggaaaagga
     2101 tgaaagtcca cgggccgaat acaaaccaac aaagcccccc actgcctttt accacccgtg
     2161 atcggagatc attgagcagg ggccctccgt ggtaagcaaa acaccgggca cgactagggt
     2221 gaacccgctt gcgccccttc gttggcggac ggtgcgcctg aatcggccgc agcaaaacct
     2281 tggagggccg tattcaggtt gctaagcgcg gtgggaagca agcgtaccaa ttccatcgta
     2341 ggatttctgg cattcagcga gtcattgagg gccttggtaa cttcatcgag tgagcgattc
     2401 agtttgctga tcaactgatc ctcgggttta ccggcaaatg ctgcccggac accacgcaag
     2461 ccctgttggg ccgagctgac gtactgggcg agtaatcgcg tttccagttt cgtccaattg
     2521 ccagtctgag acaccgtttg aagatcagcc acacaaagat cgagcaccat cttgcccagg
     2581 ccctgcggga tggtcaacaa attgacccct tggacctgcg agtaatcgaa actgcccaat
     2641 gcccgtgccg ctgccaatcg caccttcggc gattctttat cgtcggtgac aaaactaagc
     2701 agcatctgga tgttctgtcc attcggaccg gcctgcccag taatcgcgat gacatcgata
     2761 gcacggaccc gcagccattc gtggactttc ggatctttac cttccggaac gttcttgtca
     2821 ctggctagtt ggacaagaag tgggatcact tccgaatcgc ggaaatttgt atcggcaatc
     2881 ccggcgccac aatgacgctc aatccccacc aaggcggcca atcggagggc atccagctgt
     2941 tgcggatcct tcaatatcga taccaggaaa ggcaaggcac tggcaagagg caccggaggt
     3001 tgcatcccac cgcctgtcgg tcgctcacgc tcgttgagat cgccgatcgc caaaagggcg
     3061 gcaacccgca cggcaggatc gagctcgtcg ttcttgacgt aaccactcaa cgtgttgaat
     3121 gcgatttgga cgagcaaatc acgcggcgga ccaccggccc gggcgtagtt tcccaactcc
     3181 gtaacaatca attgcgatcg aagttgttgc agtttcgacc gatttgtcgc ctttgtccaa
     3241 ctcgggaaga agtacttttc gtaatagtca cggaagacat cttgaccatt ggccaccgcc
     3301 gtgaagtttc cttccttgag catccgctga acgttggccg cttgcttggc caaagaatca
     3361 tccgcggaaa gctcaagata gctcggcacc gtggggtaga cgggcttttc ctcaggtttt
     3421 tgcggggtag tccccggcaa accgggtatt cctggcaaac cgggaatggt tgttgcaggt
     3481 ggcgaaccgg gctgcacttg tgctgcggca gtgtgcaccc cgaacgttcc caaaaccacc
     3541 cagcctgcca caacgacggc acaaagaccg ctcacaaatc caaca
//
LOCUS       DAOPTR010000318         3582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_20817_length_3582_cov_3.258577, whole genome shotgun sequence.
ACCESSION   DAOPTR010000318 DAOPTR010000000
VERSION     DAOPTR010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20817_length_3582_cov_3.258577"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PKI05_07170"
     CDS             <1..295
                     /locus_tag="PKI05_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52021.1"
                     /translation="TLLPSGRTSDEEGPPQILPRSGSHEREWAAACKGGPRPMSHFGY
                     SGPLTEFLMLGNVATLFPETELEFDPIETRIINHAEADAALRRVYRRGWELSS"
     gene            333..1508
                     /locus_tag="PKI05_07175"
     CDS             333..1508
                     /locus_tag="PKI05_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOA52022.1"
                     /translation="MPRVFVVLACTLLLFTLTSLFANEEAWTLISSRNGDLEPPNGGD
                     QQTCLVVADFDNDGLLDFAVGERTKTPSVVWYQSTKQGWRRRVIEPSPLKPEAGGVAA
                     DVDKDGDVDLILGHDYSGNEICWWENPCPEFDKPWVRRAIKASGPRKHHDQTFGDFDG
                     DGRPELVSWNQGGKQLLMFPLPDHPRELKEWPSKTIFTWKQGQEYEGFPNTPVDIDGD
                     GTLDIVGGGCWFKYRGNGQFEPQVIDPTMRFTQCAAGQLIPGGFGEVVFSPGDADGEA
                     KWYQWTGKKWEAHVLGSVIHGHTCEIGDINLDGHLDILIGEMGSPGAGDRARIIVWYG
                     DGRGNFEQKVIRQGQGIHEGRLADLDGDGDLDVIVKPYHHNAPLVEILLNPIRSKGE"
     gene            complement(1684..2115)
                     /gene="rpsI"
                     /locus_tag="PKI05_07180"
     CDS             complement(1684..2115)
                     /gene="rpsI"
                     /locus_tag="PKI05_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOA52023.1"
                     /translation="MAKVVKKLVVPGKGTNDVVAVGRRKATAVARARVRPGTGIIAVN
                     GRPMEEYFPNLLYQNVALQPLIMSGRRESVDVVVRVAGGGISGQADAVKLAIARALVK
                     MDPELHKPFKDAGLLTADARKKERKKYGLHGARRGTQFSKR"
     gene            complement(2182..2652)
                     /gene="rplM"
                     /locus_tag="PKI05_07185"
     CDS             complement(2182..2652)
                     /gene="rplM"
                     /locus_tag="PKI05_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOA52024.1"
                     /translation="MKKSYLAKPGEIKPKWYVVDATDKVVGRLASDIAMILMGKHRPT
                     YTPHVDTGDFVIVTNAEKVVFTGKKWEQKKYTWYTGYPGLRSETAAERREHKPEMILW
                     DAVRRMLPKNRLARKMIRKLKIYAGPDHPHQSQQPIPIELGVKGKGKFRRLITV"
     gene            complement(2916..>3582)
                     /locus_tag="PKI05_07190"
     CDS             complement(2916..>3582)
                     /locus_tag="PKI05_07190"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HOA52025.1"
                     /translation="HYDVRPELLLISFPEKNGNVLEQDLLQKVASAAERHPWIRKVQS
                     LHKVYPGTISASVVWRQPVLMVRIPEGLLPVDAEGVLLPSRDFTPVEAAQYPRLVGLD
                     VLPAGPAGQSWGDARVVQAAELAALLFEDWKKYGFRHIELISTQITHPPEYEFVIVTH
                     SGSRIVWGHAPSVATTQEASADEKLARIEAYYRQHGSFDGIDGLQEIDVRPLGGLRVR
                     GLN"
BASE COUNT          800 a          990 c          974 g          818 t
ORIGIN      
        1 taccctccta ccatcaggaa gaacttcgga cgaagaaggg cctccgcaaa tcttgccgcg
       61 atcgggaagc cacgagcgag aatgggcggc tgcttgtaag ggtgggcccc gtcccatgtc
      121 gcattttgga tactcggggc ctctcaccga gttcttgatg ttaggaaacg ttgccacatt
      181 attcccggag acggaactgg agttcgaccc tatcgaaacg cggatcatca atcacgcgga
      241 agccgacgcc gcgttgagaa gggtatatcg tcggggttgg gaattgagtt cctgatgaat
      301 gtaagtcacc aattgctaag gggagagttg ccatgccacg agtgtttgtc gtattagctt
      361 gcacattgct cctgtttacc ttgacatctc tctttgcaaa tgaggaagcg tggacgctca
      421 taagcagtcg gaacggtgat ctcgagcctc ctaatggggg tgaccaacaa acatgcctgg
      481 ttgtagccga ttttgacaac gacggtctgc tcgattttgc cgtcggagag cgcacgaaga
      541 caccgtctgt cgtctggtat caatcgacga agcaaggctg gcgccgccgt gttattgagc
      601 cttcaccact caagccggag gccggaggtg tggcggccga tgtcgataag gatggagacg
      661 tcgacctcat cttgggtcac gattacagcg gcaatgagat ttgttggtgg gaaaatccgt
      721 gccccgaatt tgataaacct tgggtacggc gcgctattaa ggcgtcgggt ccgcggaagc
      781 atcatgatca gacatttggc gatttcgacg gtgacggacg ccccgaactg gtgagctgga
      841 accaaggtgg caagcaactt cttatgttcc ccctcccgga tcatccaagg gaacttaaag
      901 aatggccatc caagacgatc tttacctgga agcaaggcca ggaatacgag ggttttccca
      961 acacgcctgt tgatatcgat ggtgacggaa ctctggacat cgtgggcggc gggtgctggt
     1021 tcaagtaccg ggggaacggc cagtttgaac cccaagtgat tgacccgacc atgcggttca
     1081 cccagtgtgc cgcaggccaa ctcattcctg gtggttttgg agaagtggtc ttttcgcctg
     1141 gcgatgctga tggggaggca aagtggtatc aatggacggg aaaaaagtgg gaagcccacg
     1201 tcctaggctc ggtcattcat ggtcacacct gtgagatcgg cgacatcaac ctggacggac
     1261 acctggacat cctcattgga gaaatgggat caccaggggc gggcgaccga gcacgaatca
     1321 tcgtttggta tggagatggt cggggaaact ttgaacaaaa ggttattaga cagggtcagg
     1381 gaattcacga aggccgtctt gccgaccttg acggggacgg tgatttggac gtgatcgtaa
     1441 agccttatca ccataatgcc ccgctggttg agatcttgct caacccaatt cggagcaagg
     1501 gggaatagca ctggctgcct tgggagttcg aacacctttc tgagaaaaac gaaatcggct
     1561 gtgccaaccg aagtcgacac agccgattat cgaaggaggt gggctatgct tctatttcgt
     1621 tacgcaaccc atcgccaact ggttcgcgga gttgcaaaat ttttgaggca atgattcggg
     1681 aggttatcgc ttggagaact gcgtcccacg gcgggcaccg tgaaggccgt acttcttccg
     1741 ttcctttttg cgggcatcgg ctgtcaaaag ccctgcatct ttgaacggct tgtgcagctc
     1801 tggatccatt ttaaccagag cacgcgcgat tgccaacttc acggcgtctg cctgtccaga
     1861 aatgccacca ccagccacgc ggacaaccac atccacagac tcgcgtcgcc cgctcataat
     1921 taacggctgg agagcaacgt tttggtacag caaattggga aaatattcct ccatcggccg
     1981 cccgttcacg gcgatgatgc cggttcccgg ccgaacccgt gccctagcca cagccgttgc
     2041 cttccggcgt cccacggcaa ccacgtcgtt tgtccccttt cccgggacga ccagtttctt
     2101 tacgactttt gccataatag ttactaagct cgtcctgttg gtgatctttc aaatcgtttc
     2161 ggaaatcaac caaaaacctt ctcacaccgt gatcaggcgt cggaacttcc ccttaccctt
     2221 gactcccagc tcgattggga tcggctgttg gctttggtgc ggatgatcag gacctgcgta
     2281 aatcttgagc tttctgatca tctttctggc caacctgttt ttgggaagca tccgccgaac
     2341 agcatcccag agaatcatct cgggcttatg ttcgcgacgt tcagcagcgg tctcagaccg
     2401 cagaccagga taacctgtgt accaggtgta ctttttttgt tcccactttt tgcccgtaaa
     2461 gacaaccttc tcggcgttcg tcacaattac aaaatcaccg gtgtccacgt gaggggtgta
     2521 cgtgggacga tgtttgccca tcaggatcat ggcaatatcg gacgctaaac gaccgactac
     2581 cttgtcggtg gcgtccacaa cgtaccactt cggcttgatt tcaccgggtt tggccaaata
     2641 actctttttc aaagctagcc tctttctcat gaagtatccc cgatgctaca ctgatttggt
     2701 cgaccatcgg gcgatcaatc cccgctgcct gaccgcccaa aacaaccctg cgatcaaggc
     2761 agccttttct tgcgcgacat caaagactcc aatttatccc ggattggatt tggcgacaag
     2821 ggcgggggaa cctccaaaaa ggctagaaag cggccaaccg cgtacgaggt tctcttcctc
     2881 cggcttttcg ccgctcgcca ctccaggaag cggcgctaat tcaagccacg aacgcgcaat
     2941 cctcccaaag gccggacatc gatctcttgt aggccgtcga ttccatcgaa cgagccgtgt
     3001 tgccggtagt acgcctcgat gcgggccaat ttctcgtcgg ccgaggcctc ttgagtcgtc
     3061 gctaccgagg gggcatgtcc ccaaacgatg cgactgcctg agtgtgtcac gattacgaat
     3121 tcgtactccg gcggatgagt gatctgggtg gatatcaatt cgatgtggcg aaacccatat
     3181 ttcttccaat cctcgaagag gagcgccgcc aactccgcag cctgaaccac acgggcgtcc
     3241 ccccaacttt gcccggcggg cccagccggt aacacgtcca accccaccag ccgcgggtac
     3301 tgtgccgcct ctacgggcgt aaaatcgcga gacggcagta gcaccccttc tgcatcaacg
     3361 ggcaaaagcc cctcgggtat tctgaccatg aggaccggtt gccgccacac cacacttgcg
     3421 gagatcgtcc caggataaac cttgtggaga ctctgtactt ttcgaatcca tgggtggcgt
     3481 tccgcggccg acgcgacttt ttgcaagagg tcctgctcca ggacgttacc attcttttcc
     3541 gggaacgaga ttaggagtaa ttctggccgg acgtcataat ga
//
LOCUS       DAOPTR010000319         3582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_20818_length_3582_cov_2.819960, whole genome shotgun sequence.
ACCESSION   DAOPTR010000319 DAOPTR010000000
VERSION     DAOPTR010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20818_length_3582_cov_2.819960"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            348..2282
                     /locus_tag="PKI05_07195"
     CDS             348..2282
                     /locus_tag="PKI05_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007335391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HOA52026.1"
                     /translation="MDRQQVRTTWEKACDELIRRRESAGYWRGCLCSSPLATATAISA
                     LAVYSRSLESKGGSFSPTMAESIQSGLRYLLATQRPDGGWGDTERSLSNIATTYLVRA
                     AIELGGKAMVPEDALRRADQYIERQNSENGLRQRYGRDQTFVAPILTNVALAKLCSWR
                     SVPQLPFELAAFPQSLYRFLGLPVVSYAVPALVAVGLARFHLGPKGNTVVACLRRAVT
                     PKCLAVVETMQPQSGGFLEAVPLTAFVTMSLSAIGLAGHSIVQKAVRFLLSLQRSDGS
                     WPVDVDLATWVTTLATNAIAAGEYPGWMNLVSLDWILGCQHRDFHPFTGADPGGWGWT
                     DHSGSVPDADDTSGALLALANFWQKGDAETRRKILPSALLGLDWLVRLQNKDGGWPTF
                     CRGWGKLPFDRSAVDLTAHAVRAFHTWYNLLMGQPPVDLAGGRIVFDELWPDAAARRL
                     GRLENIDRALLPRLKRSISKGVAFLRQKQEHDGRWLPLWFGNERLPNEENPVYGTSRC
                     LMAYVALNQQHNPAAEKAIAWLLQAQNRDGSWGRSASEEPPRRDDETSPDAPGSIEET
                     SLAISALASFRDNPAVPAALERGLSWLTWNIENGNWRIATPIGLYFARLWYYEDLYPI
                     VFSVEALGRYLRHCDQEEKT"
     gene            2360..>3582
                     /locus_tag="PKI05_07200"
     CDS             2360..>3582
                     /locus_tag="PKI05_07200"
                     /inference="COORDINATES: protein motif:HMM:NF014078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HOA52027.1"
                     /translation="MGEELVRSQETTVQDLGSGESIKVVPQDRSCREQIRREAIRLAE
                     QLNHSELLTRDKIELTAKIVLQNLGLPQSYLGWTMVAVGSAFWRSQVMRVPYHRRLLL
                     LPHCMRNTSLCAGSYNAEGLQCASCGGCPLGWLRDVALSLGYRVLIAEGSPIVLRLIL
                     SGEADALLGVACLNSLERSLERVLMAGVPSMAVPLIVGGCRETVADEDWILEMIETPY
                     IDAESSGRSYLPLMRLAAQLVESELDGLAPRIFPTTEQREDAKVLSITEDIAYQYVKD
                     GGKYYRPFIVLSVYDALSGGKHAHRNHGDAATTMPTAVRRVALAVEVFHKASTIHDDI
                     EDDDSYRYGRPTLHKRWGIPVAINVGDYLIGLGYALLAETQDEMGKDVVADLVTLFAR
                     AHRNLCTGQGSELWLA"
BASE COUNT          781 a          963 c         1029 g          809 t
ORIGIN      
        1 attgtaatca aatccacggg caaactctcc gattttgccg atcgttcctg cctgggggtc
       61 aaacctctag tggaaggccc caaaagacaa ccattcgaac cagcaccccg ccaagaatcc
      121 gtcatttgcc aaggcggaaa gtcacttccc acaaaatgtc caatttaccg gagagcgaaa
      181 ctcggcaaaa gaaagagatc ctcgaaatca ctggccgaat gatgccgcag taatgagaat
      241 aaagaggagt tatgcctgcg tcacgcggga aacataaaac caaccgctgg ttaaaaaagg
      301 cgcactgaac aacgaggacg tcaactattt cttgggaatt ttcagctatg gatcgccagc
      361 aggtgcgaac aacgtgggaa aaggcctgcg atgaattgat ccgccgccgc gaatccgcag
      421 gatactggcg gggatgtctt tgttcctcgc cgctggcaac cgcgacggcc attagtgcgc
      481 ttgctgtgta ttccaggtcc cttgaaagca aaggtgggtc tttttccccc acaatggcgg
      541 aatccattca atccggccta aggtatctcc ttgctaccca gcggccagat gggggctggg
      601 gtgataccga gcggtcgctc agcaatatcg ccaccacata cctcgtccgc gccgcaattg
      661 agcttggcgg aaaggcgatg gtgccggagg acgccctacg tcgtgctgac cagtacattg
      721 agcgtcagaa ctcagaaaac ggcctaaggc agcgatatgg tcgtgatcag acatttgtgg
      781 cccctatctt aaccaatgtg gccctggcaa aattgtgttc gtggaggagc gtaccccagc
      841 tcccctttga attagcggcc tttccccaat ccctttatcg ttttctgggg ctgccggtgg
      901 tcagttacgc cgtgcccgcc cttgtcgccg ttgggttggc gagatttcac ctcggaccaa
      961 aggggaacac tgtcgtggct tgcctgcgac gggcggtcac accaaaatgc cttgcagtgg
     1021 tcgaaactat gcagccccaa agtggcgggt ttctcgaagc cgttcccctg accgcctttg
     1081 tcaccatgtc gctcagtgcg atcggtttgg ccggtcactc tattgtccag aaggccgttc
     1141 gctttctcct gtctttgcag cggagcgacg gaagctggcc cgtggatgtt gaccttgcta
     1201 cctgggttac cacactagcc accaatgcca ttgcagcagg ggaatatccg ggctggatga
     1261 atttggtttc cttggattgg attttgggtt gtcaacaccg cgattttcat ccgtttactg
     1321 gtgcagaccc cggtggctgg ggctggaccg atcattccgg cagtgtcccc gatgctgatg
     1381 atacgtcggg cgcgctactc gcactggcaa atttctggca gaagggagat gctgagacac
     1441 ggcgcaagat actaccctcg gctttgttgg gattagactg gctcgttcga cttcaaaaca
     1501 aagacggcgg ttggcctacg ttttgccggg gatggggaaa gttaccattc gatcgaagtg
     1561 ccgtggactt gaccgcgcat gctgtgcgag cgttccacac atggtacaat cttttgatgg
     1621 gtcaacctcc cgttgacctc gccggagggc gtatcgtttt cgacgagctc tggccagatg
     1681 ccgccgcgcg aagactgggc cgcctggaaa acatcgatcg cgcactcttg ccgcgtctga
     1741 aaagaagcat cagtaaaggc gtggcctttc ttaggcaaaa gcaggaacat gatggccgtt
     1801 ggttaccctt gtggtttggg aacgaacgtc tgcccaacga ggaaaatccg gtctacggaa
     1861 caagtcgctg tctgatggct tacgtcgccc ttaaccaaca gcacaaccca gcggctgaaa
     1921 aggctatcgc gtggctgttg caggcccaaa accgggatgg aagttgggga agatctgcaa
     1981 gcgaagaacc gccaaggcgc gacgacgaga caagcccaga tgccccagga tcgatcgagg
     2041 agacatcgct ggccatctcg gcactggcca gtttcagaga caatcctgca gtgcccgcgg
     2101 cgctggagcg gggattgagt tggctgactt ggaacattga aaacggcaat tggcgtatcg
     2161 cgacgcccat cggcctttat ttcgcacggc tgtggtatta cgaagatttg taccccattg
     2221 tgttttccgt cgaggcactc gggagatacc tccggcattg cgatcaagaa gagaaaacgt
     2281 gaatatgagc tgccctgctt ttacaatagc cattgaggcc gttcaaccac attgattctg
     2341 tgcaggtggc tacgcaatca tgggcgaaga gctggtacgc agtcaagaaa cgacagtaca
     2401 ggacctgggt tcaggcgagt ccatcaaagt ggtgccccag gatcgctcct gccgggaaca
     2461 gattcgccgg gaggccatcc gccttgcgga acagttgaat cactctgagc ttctcacccg
     2521 ggataagatc gaattgacgg ccaagatagt tctccaaaac cttgggttgc ctcaatctta
     2581 cctgggctgg acgatggtgg cggtgggatc ggccttctgg cggtcacaag tgatgcgggt
     2641 tccgtatcat cgccgcctcc tgcttttgcc ccattgcatg agaaacacca gcttgtgtgc
     2701 aggcagctat aacgctgagg gtttacagtg cgcatcgtgc ggcgggtgtc cgctgggttg
     2761 gctccgcgat gtggctttgt ccctcgggta tcgcgtgctg attgccgaag gttcaccaat
     2821 tgtgctgaga ctcatcctct cgggtgaagc cgatgcttta ctgggcgttg cctgcctcaa
     2881 ttccctggaa cggtcgctgg agcgagtctt gatggcggga gtaccgagca tggccgttcc
     2941 gctcattgtc ggcggttgtc gcgaaacagt cgctgatgag gattggatct tagaaatgat
     3001 tgagacgccc tacattgacg ccgagtcttc agggcgatcc tatttgcctc tcatgcgatt
     3061 ggccgcacag cttgtcgaaa gtgagctcga tggtcttgca ccgcgaatct tccccaccac
     3121 cgagcaacgg gaggacgcca aggtcttgtc gatcaccgaa gacattgcct atcagtacgt
     3181 taaagatggg ggaaagtatt accgtccgtt tatcgtgttg agcgtctatg atgccttgag
     3241 cggtggaaag cacgctcatc gaaaccatgg cgacgccgcc accacgatgc ccacggcggt
     3301 tcgtcgcgtt gccctcgctg tggaggtatt tcacaaagca tccacgattc acgatgacat
     3361 tgaggacgac gattcctatc ggtacgggcg tcccacgtta cacaagcgat ggggtattcc
     3421 cgtggccatt aatgtgggtg attatctgat cgggcttggg tacgcccttc tcgcagagac
     3481 gcaagatgaa atgggaaaag atgtcgtggc cgacctcgtc accctctttg cccgtgccca
     3541 caggaatctt tgtactggtc aaggctcaga gttgtggcta gc
//
LOCUS       DAOPTR010000320         3575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_20863_length_3575_cov_3.313352, whole genome shotgun sequence.
ACCESSION   DAOPTR010000320 DAOPTR010000000
VERSION     DAOPTR010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20863_length_3575_cov_3.313352"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1738
                     /locus_tag="PKI05_07205"
     CDS             <1..1738
                     /locus_tag="PKI05_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HOA52028.1"
                     /translation="RLSDAFPETGTVPGREPVVQEAGGQSANVSGSDVEVLGEYEPTT
                     ITHRRNRARLLESEEREDAVLAEAGKAAAKLCRLAFELGKATTAVRMAELALEGLFEM
                     TAVDGGAVLLVPRDFSGVATGSDLRVVASKTTSRWPYHRVSNFLASTVLREGEAVLAR
                     NVLDDSAIGSRDSRGEIHATSVICAPIRQGKRAVGVIHLYSTDPNRGPTPEDLEFTLA
                     VADTLAVALTNLNRQEELAENLTQVKTENVQLRERLGVQSELFGRSEAIRKINEEIAR
                     AAATKATVLIRGESGVGKELVARAVHFSSSRRAGPFVCINCAALSEELLASELFGHEK
                     GAFTGAFERKIGKFELAHQGTLMLDEIGEMSPGIQAKLLRVLEGHAFERVGGSQPIKV
                     DVRVIAATNRDLEQEVAQGRFRRDLFFRLRVLEIVVPPLRKRIEDIPELAYYFLAKFR
                     EETGRKIKGFTKQAMERLTSYRWPGNVRELKNVVERAVVLCKGEYIDVDDLLLSTLPT
                     TGDTAEALLQDRGYRPCSLEELERAHILATLRHTNWNKSRSAHILGIERSTLERKIRR
                     YGIDELARKNPE"
     gene            complement(1909..2817)
                     /locus_tag="PKI05_07210"
     CDS             complement(1909..2817)
                     /locus_tag="PKI05_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOA52029.1"
                     /translation="MASSILSRYLDPEVLARIARQRFEPHGLILGNLAGSHRSALSGF
                     AVEFVGHREYVLGDDPKYIDWRVYFNRGRYVVKQYAMETNLVCHFILDSSASMRYGEG
                     EQQKLQYAARAVVTLGYAILRQGDKVSLSTFDDEIRGFVPPSHSLAQVVRMTEHLDMT
                     EATRKTRMAECLYELAGRFHRREILIVLSDFFTDLGELEKAVQQIRAHRHEVIFLQIL
                     HHDEIAFEFNGPTKFVGLEQPDELITRPEELRRHYLRAFEQFQGRLVDLCQRNRVEHV
                     LVDTSRNLADVLADYLHFRNRIRRLR"
     gene            complement(2899..>3575)
                     /locus_tag="PKI05_07215"
     CDS             complement(2899..>3575)
                     /locus_tag="PKI05_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOA52030.1"
                     /translation="ADEINRTPPKTQAAMLEAMQERQVTAGGRVYRLPDPFFVLATQN
                     PLEQEGTYPLPEAQLDRFLLYIKVDYPSAAEEWEIARRVTGGQQGTIEPLMTGEELIQ
                     YQKLVTRIPISDQVLGYAWALVRASRPGSMESPDFVTRWVAWGAGPCGLLTLVTCAKA
                     RAVLHGRCHATIGDVQALLKPALRHRIAGNYAAQANNLTSDRLIEMLIEAVPPDQKYE
                     RPQCLT"
BASE COUNT          782 a          915 c         1100 g          778 t
ORIGIN      
        1 gcgattgagc gatgcctttc cggagactgg caccgttccc gggcgtgaac cggtggtgca
       61 ggaggccgga gggcagtctg cgaatgtgtc aggtagcgac gtagaggtcc tcggtgaata
      121 tgagcccacc acgatcacgc atcgccgaaa tcgtgcccgg cttctcgaat ccgaagaacg
      181 tgaagacgcc gttctagccg aggcaggcaa ggcggctgcg aagctgtgtc ggctggcatt
      241 cgaattggga aaggcgacca cagccgtgcg gatggccgag ctggctctgg aaggcttatt
      301 cgagatgacg gctgtcgatg gcggtgccgt tctcttggtg ccgcgggatt tctcaggggt
      361 ggcgacggga agtgatcttc gcgtggtggc atcgaagacg acctcgcgat ggccctatca
      421 ccgcgtttcg aatttcttgg caagcacggt ccttcgtgag ggcgaggccg ttttggccag
      481 aaatgtcctt gacgatagtg ctatcggaag tcgagatagc cgcggcgaaa tccatgccac
      541 gagtgtcata tgtgccccga ttcggcaggg taaacgggct gtcggggtaa tccaccttta
      601 ttcgacggac cccaatcggg ggcccactcc ggaagacctg gagttcacat tagccgtggc
      661 cgacacgttg gctgttgcct taacgaattt gaatcggcag gaagagctgg ccgagaacct
      721 cactcaggtc aagacggaga acgttcagtt acgggagcgg ctgggcgttc aaagcgaact
      781 ttttgggcga agtgaggcca tccgaaagat caatgaggaa atcgcacgag cggccgccac
      841 caaggccacc gtgctgatcc gcggggaaag tggtgtgggt aaagaactgg tggcccgggc
      901 ggtccatttc tcaagctcgc gtcgcgccgg tccctttgtg tgtatcaatt gcgcggcgct
      961 ttctgaggaa cttcttgcca gtgaactatt tggacatgag aagggtgcct tcactggggc
     1021 ttttgaaaga aagatcggga agtttgagct ggctcaccag gggacgctga tgctcgatga
     1081 aatcggggaa atgagtcccg gaattcaggc caagttactc cgcgttctcg aggggcacgc
     1141 cttcgagcgg gtcggcggta gccagccgat caaggtagat gttcgggtga ttgcagctac
     1201 gaatcgggat ttagagcaag aggttgctca ggggcggttt cggcgagatt tgttctttcg
     1261 gcttcgcgtg ttggaaatcg ttgtcccacc tttgcgaaaa cggatcgaag acattcctga
     1321 gctggcgtac tatttcttgg ccaagttccg cgaggagacc gggcggaaga tcaaaggctt
     1381 taccaagcag gccatggaac ggctgacctc ttatcgttgg cctggaaatg tcagggagtt
     1441 gaaaaacgtc gtcgagcggg ccgtggtcct gtgcaagggt gaatatattg acgtcgatga
     1501 tctgctgctt tcgaccttgc ccacaacggg agataccgcg gaggccttgc ttcaagaccg
     1561 cggctaccgt ccctgttcgc ttgaggagtt agagcgggca cacatcctgg cgaccttgcg
     1621 gcatacaaat tggaacaaga gccgttccgc ccacattctc ggcattgaac ggtcaaccct
     1681 cgaacggaag atccgccgtt acggaattga tgaattggct cggaagaatc cggagtgagt
     1741 tttccgaaaa ctcggcgagt tgggaactta ttcgggacgc ctgatgcaaa acgggggcaa
     1801 ccatattttt caaggatgaa ggccaactcg gcatgtttta tcgaagactg tgtgccacgg
     1861 ttcacggcga tggagcgtcg tgtcacgttg gggaaatcac tcccccaatc aacgaagacg
     1921 tctaattcgg ttacgaaagt gcagataatc ggccaggaca tcagcgagat tcctgctggt
     1981 atcgacgaga acgtgttcga cacggtttcg ctggcagaga tcgacaaggc gtccttggaa
     2041 ctgctcaaat gcccggaggt agtgccttcg cagctcctcg ggtcgagtga taagctcatc
     2101 tggctgttct agcccgacaa actttgtggg tccgttgaac tcgaaggcga tttcgtcgtg
     2161 gtggaggatt tggagaaaga ttacctcatg gcgatgcgca cggatttgct gaacggcttt
     2221 ttccaattca ccaaggtccg tgaagaaatc gctaaggacg atcaggatct cgcgccggtg
     2281 aaatcgtcct gccagttcgt agaggcactc ggccattctg gtctttcgag tggcttcggt
     2341 catgtccaga tgttctgtca tcctcacaac ctgggcaagc gaatggctag gcgggacgaa
     2401 cccccgaatc tcatcgtcga aggtcgagag tgatactttg tcaccctggc ggaggatcgc
     2461 ataccccagt gtgaccacag cgcgggccgc atattgaagt ttttgctgct cgccttcgcc
     2521 atagcgcatg gaagcactgc tgtcgagaat gaaatgacag actaaattcg tttccatggc
     2581 gtattgtttg acgacgtagc ggccacggtt gaaatagacc cgccaatcga tgtacttggg
     2641 atcatcaccc aagacgtatt cccgatgccc tacaaactcg acggcgaacc cggaaagcgc
     2701 ggagcgatgc gaaccagcaa ggtttccgag gatcagaccg tgcggttcaa acctttgacg
     2761 ggcgatgcgg gctagcactt cgggatcgag gtagcgggaa aggatcgagc ttgccatgtc
     2821 aaattcgtat tgacctcgtt taactcacgt tacttgggaa gaggcatgcg ccatgaatca
     2881 acggtaactt gtgggtcatc acgtcaaaca ctgtggtctt tcatactttt ggtcaggggg
     2941 gaccgcctcg atcagcattt cgatcaggcg atcactggtg aggttatttg cctgagcggc
     3001 atagttgccg gcgatacggt gacgcaaagc gggcttgagg agggcttgga catcgccgat
     3061 ggtggcatga catcggccgt gcaagacggc gcgggccttg gcacaagtca caagggtcaa
     3121 cagaccacat ggacccgccc cccaagccac ccagcgggtc acgaaatcgg ggctttccat
     3181 cgatccggga cgagaggcac gaaccaaggc ccaggcataa cccagtactt ggtcggaaat
     3241 cggaatgcgg gtcacaagtt tctgatactg aatcagctcc tcaccggtca tcaatggctc
     3301 aattgttccc tgttgtcctc ccgtaacacg gcgggcgatt tcccattctt cggcagcgga
     3361 cggataatcc accttaatgt aaagcagaaa acggtcgagc tgtgcctccg gaaggggata
     3421 ggtcccctct tgctcgaggg ggttctgtgt ggccagcaca aagaacgggt cgggaaggcg
     3481 gtacacgcgg ccacctgcgg tcacctgccg ctcttgcatg gcttccaaca tcgcggcctg
     3541 cgtcttgggt ggtgttcggt tgatctcgtc ggcca
//
LOCUS       DAOPTR010000321         3566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_20948_length_3566_cov_2.604101, whole genome shotgun sequence.
ACCESSION   DAOPTR010000321 DAOPTR010000000
VERSION     DAOPTR010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20948_length_3566_cov_2.604101"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PKI05_07220"
     CDS             complement(<1..564)
                     /locus_tag="PKI05_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52031.1"
                     /translation="MDFQTTLVSIWREACRHIEITESAAAIAQVLSAQMRFQTLVVRR
                     LDLSQMQLQTVAVAPKSPPGLGPRTTPLSRVSMSRLLDWFSRRVIWNIPQDGAFDGLD
                     AHELFGEIHATMLIGGLLAGQTPAGILAVVEEPNRAAFSEEDVRLVQQLLEPFAVALE
                     NDQRLREMATLREAAEAEKRSLLAKLGR"
     gene            847..1950
                     /locus_tag="PKI05_07225"
     CDS             847..1950
                     /locus_tag="PKI05_07225"
                     /inference="COORDINATES: protein motif:HMM:NF008106.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOA52032.1"
                     /translation="MHAEGKCNSTENKRWCATVLFGVILVTAVLAVTGCARRGNALVL
                     LNVSYDPTRELYQEINEAFAKDWFEKTGETVEIRQSHGGSGKQAHAVMEGLEADVVTL
                     ALAYDIDSIATRTQLIDQNWQDRLPHRSCPYVSTIVFLVRKGNPKNIRDWNDLTRPDV
                     EVVTPNPKTSGGARWNYLAAWGYALRRSLGDLKKAKDLNSPEVAQAQKEAKEFVRQIF
                     ARVKVMDTGARAATNTFAQRGIGDVLIAWENEALLTVKELGADKFEIVVPSVSILAEP
                     PVAVVDQVVDRRGTRQVAEAYLQFLFGIEAQRIIARHYYRPTLPEVAQEFAGQFPQLE
                     LFTIDDVFGGWQTAQKEHFDDGGLFDQIMAATR"
     gene            1960..2820
                     /gene="cysT"
                     /locus_tag="PKI05_07230"
     CDS             1960..2820
                     /gene="cysT"
                     /locus_tag="PKI05_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HOA52033.1"
                     /translation="MTGRIDQYGFRGLFRDDVLPGFWLTFGITIAYLGVMVLLPFAAL
                     VAFVTGSADGWQVLQESRVWAAVRLSVGGALVAALIDAVLGVLTAWVLVRYRFPGRQL
                     LDALVDLPFALPTAVSGIALTTLFAPKGWLGAWLQPLGIQVAYTQLGILVALVFIGLP
                     FVVRAVQPAIEDLDPEWEEAAASLGASRWATFWRVIFPSLWPSVLTGFAMAFARGVGE
                     YGSVIFIAGNLPHRTEIAPLLIVIKLEQYDYHGAAVIATIMLLVSLGVLLAVGAFQNW
                     SQPWQKAATH"
     gene            2832..>3566
                     /locus_tag="PKI05_07235"
     CDS             2832..>3566
                     /locus_tag="PKI05_07235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit"
                     /protein_id="HOA52034.1"
                     /translation="MVSCELTDRGLQNGPGVLDRAHDDLPSVPVRHQTPAATGSLSRL
                     LLTGIVVAFLGTFLVLPLIVVFVEAFRHGVTVYLAAITDRDALAAIRLTLFVAVAAIT
                     INTAFGLAAAWCLGKFRFPGRQLIIALIDLPISVSPVVVGLLLVLVYGRHGWLGSFLE
                     GHGLRIIFATPGIVLATAFVTLPLVVRELIPTMDAQGREQEEAARLLGANGWQVFLYV
                     TLPSIRWALLYGVILCTARAVGEFGAV"
BASE COUNT          679 a          912 c         1102 g          873 t
ORIGIN      
        1 ccgaccgagt ttcgccagca gtgaccgctt ttctgcctcg gcagcttctc gcagggtggc
       61 catctcgcgc aatcgttggt cattttccag agcaacggca aacggttcga gaagttgctg
      121 gaccagacga acatcttcct cggagaaggc cgcccggttg ggttcctcca cgaccgctag
      181 gatccccgcg ggcgtttgtc cagccaacaa gccaccgatg agcatcgtgg cgtggatttc
      241 gccaaacagt tcgtgggcat cgagcccatc aaatgcccca tcttggggga tattccatat
      301 aacgcgccga gaaaaccaat caagaagccg ggacatggat accctggaga ggggtgttgt
      361 tcgggggccc aagcccggtg ggctcttcgg agccacggct acggtttgca actgcatctg
      421 gctgaggtcg agtcgccgca cgacgagtgt ctgaaaccgc atttgggcgg acaaaacttg
      481 ggcgatggcc gccgccgact ccgtgatctc gatatgccgg caggcttccc gccagatgct
      541 cacaagcgtt gtctgaaagt ccatggaaag gggccctttc gcaatcgaat ctcgctcgac
      601 tcggggaatc taacagcgtg cccattatat ttcacggcga gccgttattc tagacggatc
      661 cccgccgtga tatttcacgg ctagatcgcc gttcttctct ggtggatgca taaattctta
      721 cctaaaaaca acttgcgatc ttgctgggaa cacggcacgg tgtttgcagt cagtaagccg
      781 tctttgctcc gggctaggtt gtaggccaag caacaaatat gcctaactaa attggtgttt
      841 tacgccatgc atgccgaggg gaagtgcaac tcgacggaga acaaacggtg gtgtgccacg
      901 gttctattcg gcgtaatcct cgtgacagcg gtgctggctg taacgggatg cgctcgacgc
      961 ggcaatgcgc tggtcctttt gaatgtgtcc tatgacccaa ctcgggagct ttatcaggaa
     1021 atcaacgagg ccttcgccaa ggattggttt gaaaaaacgg gcgagacggt ggagattcgg
     1081 cagtctcatg gtggttctgg caaacaagca cacgcggtga tggaggggct cgaggcggat
     1141 gtcgtaaccc tagccttggc ttatgacatc gacagtatcg ccacgcggac gcaactgatc
     1201 gaccaaaact ggcaggaccg tctgccccat cgaagctgtc cctatgtttc gacgatcgtt
     1261 tttctcgtgc gaaagggaaa tcctaaaaac atccgggact ggaacgacct gacgcgaccg
     1321 gatgtggaag tcgtaacgcc gaatcctaag acctcaggcg gggcgcgctg gaattatctc
     1381 gcagcctggg ggtacgccct aagacgatcg cttggcgatc tgaaaaaggc caaggacttg
     1441 aattcgcccg aggttgccca ggcccagaaa gaagccaagg agtttgttcg gcaaatcttc
     1501 gcccgggtta aggtcatgga taccggagcc agggccgcaa cgaacacgtt cgcccaaagg
     1561 ggtatcggcg acgtgctgat agcctgggag aatgaggccc tgctgacggt caaggaactg
     1621 ggggcggaca agtttgagat tgtcgtgccc agtgtcagca tccttgccga accgccagta
     1681 gccgtcgtgg atcaagttgt cgatcgccgg ggaacgaggc aggtggcaga ggcttatctt
     1741 cagttccttt ttgggatcga ggcacagcgg atcattgcgc gacattatta tcgaccgact
     1801 ctgcctgagg tagcccagga gttcgcaggg caattccctc aattggaact attcacaatt
     1861 gatgatgtct ttgggggttg gcaaaccgct cagaaagagc actttgatga tggcggtctt
     1921 tttgaccaaa tcatggcagc gacacggtga gataaggtga tgaccggtag gatcgatcaa
     1981 tacggctttc gcggcctttt tcgggatgat gtcttgccgg gcttttggct gacattcggg
     2041 atcactattg cctacctggg agtgatggtc cttctgccct ttgccgcttt ggtcgctttt
     2101 gtgacgggta gtgccgacgg ctggcaagtc ttgcaagagt caagagtctg ggcagccgtc
     2161 cgcttgagtg tgggtggcgc tctggtggct gccttgattg atgccgtttt gggcgtgctg
     2221 acggcctggg tccttgtgcg gtataggttt ccagggcggc aattgctaga tgccttggtc
     2281 gatttgccgt ttgcccttcc cacggccgtc tcggggattg cactgaccac gttgtttgcc
     2341 ccgaaggggt ggctgggagc ttggctacaa cccttgggga ttcaggtggc ctatacgcaa
     2401 cttgggattc tcgtcgcgct ggtgttcatc ggtttaccct ttgtggttcg agccgttcag
     2461 ccggccatcg aggatttgga cccggaatgg gaagaagctg cggccagttt gggtgcttca
     2521 agatgggcaa ccttttggcg ggtcatattt cctagccttt ggccctccgt gttgacgggg
     2581 tttgcgatgg cctttgcacg tggggtgggc gaatatgggt cggtgatctt cattgctggg
     2641 aatctccccc atcgcacaga gatcgctccg cttttgatcg tgattaagtt ggagcagtac
     2701 gactatcacg gggcggccgt gattgcgacg atcatgttgt tggtttccct tggggtgctg
     2761 cttgccgtgg gagccttcca gaattggagt cagccatggc aaaaggccgc aacacattga
     2821 gcagataaac catggtttca tgcgaattga cagatcgagg gttgcaaaac ggtccgggtg
     2881 tcctggatag ggcccacgat gacttaccga gtgtgcctgt tcgtcaccaa acaccggccg
     2941 ccacggggtc tttgtcgcgg ctattgttga cgggcatcgt cgttgcgttc ttgggaacct
     3001 ttttggtatt gccgttgatc gtggtctttg tggaggcctt tcgtcacggt gtcaccgtct
     3061 atcttgccgc gatcaccgat cgcgatgccc ttgcggccat taggttgacg ctgttcgtag
     3121 ccgtagcggc gatcacaatc aacacggcct ttggattggc ggcagcctgg tgtttgggga
     3181 agtttcgttt tcccgggcgg caattgatca tcgcccttat tgatttgccg atttctgttt
     3241 cgcccgtagt ggttgggctg ctattggtcc tcgtttatgg tcgtcacggc tggttgggta
     3301 gtttcttgga agggcacggc ctgcggatca tttttgctac gccgggcatt gtcttggcta
     3361 cggccttcgt cacgttgccg cttgtagttc gggaattgat ccccacgatg gatgcccaag
     3421 gacgcgaaca ggaggaggcc gcccggctcc ttggtgccaa tggatggcag gtctttctgt
     3481 acgtcacgct gcccagcatt cggtgggctt tgctgtacgg ggtgattttg tgcacggcgc
     3541 gggccgtggg cgaatttggg gccgtc
//
LOCUS       DAOPTR010000322         3565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_20958_length_3565_cov_3.295726, whole genome shotgun sequence.
ACCESSION   DAOPTR010000322 DAOPTR010000000
VERSION     DAOPTR010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20958_length_3565_cov_3.295726"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PKI05_07240"
     CDS             complement(<1..255)
                     /locus_tag="PKI05_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52035.1"
                     /translation="MLIAEGYDTRDPEGYKTPPPMIALSFKSPLIDLCSSGRHYGVKV
                     DDISLLRLITWVDAMCPYQGEEEIREIPDPEFQGIDWLAIR"
     gene            complement(510..1538)
                     /locus_tag="PKI05_07245"
     CDS             complement(510..1538)
                     /locus_tag="PKI05_07245"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOA52036.1"
                     /translation="MGILTGWLDLVFPPVCVVCGKPVAIETGLAEWRPLLEERVRRHP
                     DRAYDLLWLGGKPPQPGVSNFLCPHCLAILSFSGWISCPKCGGQVDDESPSPDGCDWC
                     RRERFAFKGVVSLGPYAGPLGEVILRMKHPSGERLAHCMARLFCCERGEKLRELAIDC
                     VVPIPLFARQRRRRRINSPEILAETIAKYLGVSLETTLLRRVRDTRPQRTLKPRERWK
                     NVRDAFSVEVSIHDVWRSKLATFWHHAIIRQGPTADTSHDQDEHAKLTSAVRANRGPY
                     HPRLRDRRVLLVDDVLTTGATCHAAAEALLRAGAREVFVAVLARGQGDDDSVAAAMPL
                     HETEFRPW"
     gene            complement(1895..2785)
                     /locus_tag="PKI05_07250"
     CDS             complement(1895..2785)
                     /locus_tag="PKI05_07250"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOA52037.1"
                     /translation="MAHMTRRGILVGGLATGVGLVTRRLTWAHGLAEVSSQKAIRLGL
                     VTYLWGRDWDLPTLLANCEKTGMEGVELRTEHAHGVEPEIDAAKRAEVKKRFADSPVV
                     LVGLGSNEAFHYPNPEQLRRAIERTKAFIRLSHDVGGLGVKVKPNDLPKEVPQEKTIE
                     QIGKALNELAAFAADYGQKLRLEVHGQCAPLPIMRKIMDIATHPNVGVCWNSNAEDLQ
                     GEGLEYNFNLVKHRLADTVHVRELNIGDYPYQKLFDLLVANGYKGWILLECRTNPPDR
                     VAALIEQRKVWEEMMAKARG"
     gene            2935..>3565
                     /locus_tag="PKI05_07255"
     CDS             2935..>3565
                     /locus_tag="PKI05_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52038.1"
                     /translation="MQNRNVVQHLPGTVFGNFVFRDGITRSAVILALGLLIWVICLDQ
                     VTWSAESARLTERQLIVSNDAQLRLAIRQARPGITIRIQGGQYPGELWIENLHGRPDA
                     PIVIEAADPQNPPVFVGGQTGLQIARGSYFTLRYLKFRGQKVNGINIDDGGDIDSPTH
                     HIRLEHLVVEDVGPQGNFDGIKLSGVTDFVVTNCRIEGWGGQAIDMVGCH"
BASE COUNT          804 a         1049 c          939 g          773 t
ORIGIN      
        1 gcggatggcc agccaatcaa tgccttgaaa ctcaggatcg ggaatttcgc ggatttcttc
       61 ttccccttga tagggacaca ttgcatccac ccaggtaatg agtcgcaaaa ggctgatgtc
      121 atcaaccttg accccgtagt gacgaccgct tgagcaaaga tcaatgagcg gacttttgaa
      181 ggaaagagcg atcatcggcg gcggggtttt gtaaccctcg ggatcgcggg tatcatagcc
      241 ttccgcgatt aacatgccag caatcccgaa ccctgggggg accacatggg gtttgcgata
      301 aggttgcccc aagtcggctg accaatgggc aaaaagtatg gttcggcgaa tcccagaaaa
      361 cccggtcgcg cggtcaaatc taagaccttg accgcctacc cttcgtaata tgccgcggcg
      421 tcgcaacgcc tgagccccat cgtaagaagg ggtgcttttt gatcctctgt gacggtgata
      481 tccgcttcct ccagtcgacc tgcgggcaat caccaggggc gaaactcggt ctcgtgcaga
      541 ggcatggccg ccgccaccga atcgtcatcc ccttggcccc gtgccagaac ggcgacgaag
      601 acctcacgcg cccctgcccg caaaagggcc tcggccgcgg cgtgacatgt cgccccggtc
      661 gtcaaaacat catcaactag gaggacccga cgatcccgca accgaggatg gtatggccca
      721 cggttagcgc gaaccgcgga tgttaacttt gcgtgctcgt cctgatcgtg gctcgtatca
      781 gcagtgggcc cctgtcggat aatcgcgtgg tgccaaaatg tcgccaactt cgacctccat
      841 acgtcgtgga tacttacttc cacggaaaag gcgtcgcgga catttttcca ccgctctcgg
      901 ggtttcaatg tccgttgcgg gcgggtgtcg cggacccgtc gaagaagagt ggtttccaat
      961 gatacgccaa ggtacttagc gatggtttcg gccaggattt ccgggctgtt aatacgacga
     1021 cggcgtcgtt gtcgcgcaaa gagaggaata ggaactacac agtcgatcgc aagctcacgg
     1081 agtttctcgc ctctttcgca gcaaaaaagc cgcgccatgc aatgcgctag ccgttcgccg
     1141 ctgggatgct tcatcctgag aatgacctcg cccaatggcc ccgcatatgg ccctaacgaa
     1201 acgacacctt taaacgcaaa gcgttcccgc cggcaccagt cgcaaccgtc aggcgagggc
     1261 gattcgtcgt ccacctggcc accacacttg ggacaactta tccaacccga aaacgaaaga
     1321 atcgccaaac aatgtggaca gaggaaattg ctcacgccgg gctgtggtgg ttttccgcca
     1381 agccaaagca gatcataagc ccgatcggga tgacgccgca ccctctcctc gagcaaaggc
     1441 cgccactcgg ccaagcccgt ttcgatagct acgggcttcc cacagacaac gcacaccggc
     1501 ggaaagacga gatcaagcca gcccgtcaat attcccaagc gaaacgatct ttcactgcct
     1561 ctgtgtggct gttgcatatg ccaaatggag aaagcggaca tagagcctat ccctaatagt
     1621 ccccctctcc ctccgggaga gggctggggt gagggcaagc gaacgtcgga atctatcgag
     1681 caaacccaca gcgattcaag ccaccctcac ccccgacccc ctcccgccga aaaagcgagg
     1741 ggagagtgtg ggataggctc ttaccccatc atctcgggca aacccatccg gtgtatcact
     1801 cgcgccggcc tgatattggc cgcgtctgct ttgtcattct attactgaac aacgactgtt
     1861 gccatgcttg gaagacaatg tccgcgcaaa tcgtctatcc gcgggccttg gccatcatct
     1921 cttcccaaac ttttcgctgt tcgattagag cggcgacgcg atcgggcgga ttcgttcggc
     1981 actccagaag aatccaaccc ttgtagccgt ttgctaccag gaggtcaaac agcttctgat
     2041 atgggtaatc tcctatattc agttcccgca cgtggacggt atccgccaga cggtgcttaa
     2101 cgagattaaa gttgtactcc aacccctcgc cttgcaaatc ttcggcgttc gagttccagc
     2161 aaacgccgac attgggatgg gtggcaatgt ccatgatctt tctcatgatg ggcaacggcg
     2221 cgcactggcc gtggacctcc aaccgcagct tctgcccata atctgccgca aaggccgcca
     2281 attcgttcaa ggctttacca atctgctcaa tagtcttttc ctggggtacc tcttttggca
     2341 gatcgttggg cttcaccttg acgccaagcc ccccgacatc atggctcaag cggatgaaag
     2401 ccttcgtccg ttcgatagca cgtctgagtt gctcagggtt gggataatgg aaggcctcgt
     2461 tgcttcccag gcctaccaga accacgggac tgtccgcaaa gcgtttttta acctcggcac
     2521 gcttggctgc gtcgatttct ggctcgacac cgtgggcgtg ttccgtgcga agctcaacac
     2581 cctccatgcc cgttttctca cagttagcaa ggagcgtagg caaatcccag tcacgtcccc
     2641 acagatatgt taccaaccca aggcggatcg ccttttgaga agatacctca gccaaaccat
     2701 gtgcccaggt caaccgccga gtaaccaaac cgactcccgt tgccagacct cccacaagaa
     2761 tccctcgccg tgtcatgtgt gccatggacg attcctcctc aaatcatgtg acagtgtatc
     2821 gcctcgacga taagatattg tacactgagt ttgcttgaca atcccatcgt ccgcaaaccc
     2881 cgttatcgct tgtgagcgtc gtcatcgccc aatgacacat gagggaggaa agccatgcaa
     2941 aacaggaacg ttgtgcaaca tctaccggga acggtgtttg gaaatttcgt tttccgcgac
     3001 gggattaccc gaagtgccgt cattttggcc ttgggtttat tgatttgggt aatttgcctg
     3061 gaccaggtaa cgtggtctgc tgagtcggcc cgtcttacgg agcgtcaact gatcgttagc
     3121 aatgacgcgc aactccggct ggcgattcga caggcacggc caggaatcac cattcgcatt
     3181 caagggggac aatatcctgg tgagttatgg atcgaaaacc tgcacggacg gcctgatgcg
     3241 cccatcgtta tcgaagctgc cgatccgcag aatccgcccg tttttgtcgg tgggcaaacc
     3301 ggtttgcaga ttgccagggg cagttatttt acccttcgtt atctcaagtt tcgtggccaa
     3361 aaggtcaacg ggataaatat cgacgacggc ggcgatatcg actcgccgac tcaccacatc
     3421 cgacttgagc atcttgtcgt ggaggatgtt ggtccgcaag gcaatttcga tggtatcaaa
     3481 ctatccggcg tgacggattt cgtcgtcacc aactgtcgca ttgaaggctg gggcggacag
     3541 gcgatcgata tggtcggctg tcatc
//
LOCUS       DAOPTR010000323         3561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_20984_length_3561_cov_3.582145, whole genome shotgun sequence.
ACCESSION   DAOPTR010000323 DAOPTR010000000
VERSION     DAOPTR010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20984_length_3561_cov_3.582145"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="PKI05_07260"
     CDS             complement(<1..551)
                     /locus_tag="PKI05_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52039.1"
                     /translation="MPSMFEEFLQAARSIFEQAQHEGRLIRNPDTARLRAMALEEPEA
                     RETCYGNVAVESEPTSRAAMFTKNNIDTRFGKEEYDLLEKAKRELSKKELVSIDVQVG
                     DGTEGVTARLIVPRDFAHVAFAGLKLFKPTSTDEPTYQVIMFTDEAFESNKKKKLPDK
                     DITIRLAHAPDGRMVKFVRNSNY"
     gene            1191..2246
                     /locus_tag="PKI05_07265"
     CDS             1191..2246
                     /locus_tag="PKI05_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOA52040.1"
                     /translation="MTVCVGRCGRKPRWGFTLVELLVIIAIIGILIALLLPAVQAARE
                     AARRSQCTNNLKQLALAMQNYHDVYTRFPARQAGSGHQNTGSNDTHRGRLSGWVAVCP
                     YMEQQSLYDQINALPHQAPWNNTSWYNVNIPALNCPSDPASTPPTGTARGTVTYCMNG
                     GDSPIDSFNNPPSSTSIRGVFHVFQWSSMAEISDGTSNTAVISERLRPRVQNSLGNVV
                     VLSLGTTPLTCRAAYDSSQRKYITGSFTTGRVPGFGWADGAASFAGFTTILPPNSASC
                     ANAADHWERGIYTPSSRHPGGVNVAFADGSVRFVSETVDTGNLGTDFPSVTSGGMSPY
                     GVWGALGTKSGGETASL"
     gene            2431..2901
                     /locus_tag="PKI05_07270"
     CDS             2431..2901
                     /locus_tag="PKI05_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52041.1"
                     /translation="MTKTRNVFSVTCLALVGLALLFFALPGCGGKRSVPGRPKTVPVK
                     VTVLYRGAPVAEANVQFLPEGSGNAATGVTDANGIARLTTFDQNDGAVPGKYRVTIRK
                     SIQVGGSNPADPDAPPVPATYREELPAKYAAPETSGLTAEVSESQKEFTFELTD"
     gene            complement(2923..3195)
                     /locus_tag="PKI05_07275"
     CDS             complement(2923..3195)
                     /locus_tag="PKI05_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52042.1"
                     /translation="MLHRLLRGPSGNGPWKCNITDQQKMGQEHRPTDAFLAVTREGRT
                     PRCPMWYEPPGQTGETCHLQFAAARDSRWATAVYVFTTDPTAEFAA"
     gene            3235..>3561
                     /locus_tag="PKI05_07280"
     CDS             3235..>3561
                     /locus_tag="PKI05_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOA52043.1"
                     /translation="MTREPILKPSVSTDEDERLLRPQRMEEMVGQRDVYERLQIAIDA
                     ALKRDEPLGHILFDGPPGLGKTTFALCIPRHLGVSIQIASGATLKAPKDLIPYLTNAE
                     ERSVLFI"
BASE COUNT          717 a         1019 c         1011 g          814 t
ORIGIN      
        1 aagtaattgc tattccgaac gaacttaacc atccgcccat ccggggcatg cgccaaacga
       61 atggtaatgt ccttatcagg aagcttcttc tttttgttac tctcaaatgc ttcatcggtg
      121 aacatgatga cctgataggt cggctcgtcc gtgctcgttg gcttgaaaag cttcagcccg
      181 gcaaaggcga cgtgggcaaa atctcgcggg acgatgaggc gggcggtcac gccttccgtt
      241 ccatctccga cctgcacgtc gatgctcacc aattcttttt ttgaaagctc ccgcttggcc
      301 ttttccaaca ggtcatactc ttccttgcca aatctcgtat cgatattgtt ctttgtgaac
      361 atggcagccc gggaagttgg ctccgattcc acggccacgt tgccgtagca cgtctcacgg
      421 gcctctggct cctcaagggc cattgcccgg agtcgggccg tgtctggatt gcgaatcaac
      481 cggccctcgt gctgagcctg ctcaaaaatc gaacgcgctg cctggaggaa ttcttcaaac
      541 atcgatggca tctcccgaaa acaccttcga ctgtgagtac agtcacataa acccgttggg
      601 acgctgtatg taagcgcgca ctatggatct ttcctgccct tggcacatcc ttttcacgtt
      661 atcccgatag ccgccgcgcc acaatccccc cgacccggcg ttatcatgcc caggagtcgg
      721 cagcagcagg ttttgacggg agcgccgagt gagaattttc cgctggcggc acctgcaaca
      781 tcggagacca agatgcgtgt ccccccgttt ttgaggtggg accggcaatc gcctgcacgg
      841 gccaggggcc gtggaggccg cgtgattgaa atccgtttcg gacccaaacg ggcaatcaca
      901 agagttgccc ccagcgtcaa ggtacagcca gcgcatacaa atcaccggcg cgccgtcgct
      961 tgacaatagt gccccgggga cctaagatgc aatatggaag ggaacgataa ctgcaccctc
     1021 agtttctccc gcctttcacg tgaaggggct cgtcttggtc gtgatcttgt ttttgtgatc
     1081 ccgagacggt tcaggggtat cggttcagtt ttagcattgg cttggtggaa gttctcgcac
     1141 tgtgtttcga gatgcttcct tgttgttcct gtttggagga ggtttgtgag atgacggtat
     1201 gtgtcggtcg ttgtgggcgt aagcctcgtt ggggcttcac gctggttgaa ctgttggtca
     1261 ttattgccat tatcgggatc ttgatcgcct tgctgttgcc ggcggttcag gctgcccggg
     1321 aagccgcacg aaggagccag tgcaccaata acttgaagca acttgctttg gcgatgcaga
     1381 attaccatga tgtgtacacc cgttttcccg cccgccaggc tggatcggga catcagaata
     1441 cgggcagcaa tgatacccat cggggtcggc tgagcggttg ggtggccgtc tgcccctaca
     1501 tggaacagca gtcgctctat gatcagatca atgccttgcc ccaccaggcg ccatggaata
     1561 acacctcctg gtacaacgtg aatatcccgg ccctgaattg ccccagtgat cccgcctcga
     1621 cacccccgac cggcactgcc cggggcactg tcacttactg catgaacggc ggggattcac
     1681 ccatcgacag cttcaataac ccgccgagta gcacgagcat ccgcggtgtg ttccatgtct
     1741 ttcaatggtc gagcatggct gagatcagcg acgggacgag caatacggcg gtgatctctg
     1801 aacggcttcg tccgcgtgtt caaaactcgt tgggaaatgt cgtggtactg tccctgggaa
     1861 caaccccgct gacctgccga gcagcttatg actcctcgca aaggaagtac atcaccggca
     1921 gttttacaac gggtagggtc cccggtttcg gttgggcgga tggagcggcc tcgtttgccg
     1981 ggtttaccac cattctcccg cctaattccg cttcctgcgc gaatgccgcc gaccactggg
     2041 agcggggcat ttacacgcca agcagccgtc acccaggcgg tgtcaatgtc gcctttgccg
     2101 atggttccgt ccgctttgtc tctgaaacag tggatactgg aaacctgggc acagactttc
     2161 cctcggtcac ttccggtggt atgagcccct acggtgtttg gggtgccctt ggcaccaaga
     2221 gcggcggcga aacggcaagc ctgtaaaatc ggggtgtacc cgtaacgaca ttggttcgtt
     2281 gaggtttcag ccgatcggga tttcaggtta tcagtgaaac cccctctccg gtttgattga
     2341 gagggggttt ccgtcgttaa ccgttcaggg ggaccagctc cctgacaata tttcaacaaa
     2401 cggtaggtga gaagggagtt ttgagagacc atgacaaaaa ctcgcaatgt cttttcggtg
     2461 acgtgcttgg ctctggtggg acttgcactg ttgtttttcg ctttgcccgg ttgtggtggc
     2521 aagcgatcgg tccccggtcg ccccaagacc gtccccgtca aagttacggt cctctatcgt
     2581 ggtgctcccg tagcagaagc gaacgttcag tttctgccag agggcagtgg caacgcagcc
     2641 actggcgtta ctgacgccaa cgggatcgcc cggttgacca catttgatca aaacgatggc
     2701 gccgttcccg gcaaatatcg tgtgaccatc cgcaagtcaa tccaagtcgg tggttcgaat
     2761 ccggccgatc cggatgcccc gcctgttccc gcaacgtacc gcgaggagct gcccgccaag
     2821 tatgctgctc cggagacatc tggattaacg gccgaggtca gcgaatccca gaaggagttc
     2881 acgttcgagc tgacggactg actgataccg aaggccagcc gattaagcgg cgaattcagc
     2941 cgtggggtcc gtcgtgaata cgtacacggc ggtcgcccac ctcgaatcac gagccgccgc
     3001 aaattggagg tgacatgtct cgccagtttg gcccggcggc tcgtaccaca ttggacatcg
     3061 cggcgtgcgt ccctctcggg tcacggccaa aaaggcatcc gtaggccgat gctcttggcc
     3121 catcttctgc tggtctgtta tattgcattt ccaggggcca ttgccgcttg gtccccgcag
     3181 aagccggtgg agcatggcgg agcctggatc agaaagacgg attgacgtgc ggaaatgact
     3241 cgcgaaccca ttttgaagcc gtccgttagt acggatgagg atgagcggct cctccgtcca
     3301 caacggatgg aggaaatggt cggtcagcgc gatgtgtacg aacgattgca gattgcgatt
     3361 gacgcagccc tcaaacggga cgaaccgctg ggacacatcc tctttgatgg accgcccggg
     3421 ttgggcaaaa ccacctttgc tttgtgtatt ccgcggcatc tgggggtcag cattcagatc
     3481 gccagcggag ccacgctcaa ggccccgaag gacttgatcc cctatctgac gaatgccgaa
     3541 gagcgatccg tccttttcat c
//
LOCUS       DAOPTR010000324         3560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_20990_length_3560_cov_3.373466, whole genome shotgun sequence.
ACCESSION   DAOPTR010000324 DAOPTR010000000
VERSION     DAOPTR010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20990_length_3560_cov_3.373466"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /locus_tag="PKI05_07285"
     CDS             <1..1449
                     /locus_tag="PKI05_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52044.1"
                     /translation="REASECVLTLLESFFDLNLMNDAKEFLERIPLESSGRARAELRL
                     GQVLWTQYVKLAASQDARPSPEESDAILQEARRYLEQGLQKIRSGGGEVDYLVEYSVL
                     LLAQLLVNTGETDQAITLLEDATLGPLTLIRQGHAAVENAKLREEVMKVALRAYVGSQ
                     NLDKAFEVQEQLEKLVAGSGDAEATKRLTAVYVGLGRQLEEQLARLRSEGKTAEMEKV
                     LEGFTKFLEAIQERSASVDFRALNWVAETFLGLAKGLDPGGPQVPEQAKEYYKKAVET
                     YIQLIKRCEAEPSFGPEDAPLFLRVRLAAALRGWRLFDKAMEAILFVIDKQGKENRLD
                     AQLEAAQIYQEWAEQPGKEQYYEYAIRGGHEKNGRYLIWGWSGIARRVASDYNRFLDV
                     FHQARYNLAYCRFKLAQKATGAKRDQLLKEAELDIQRTYLLYPSLGGKEWYAKYDKLL
                     RTIQQLRGEKNPQGLKAFESRPRATPASSKAG"
     gene            1512..2534
                     /locus_tag="PKI05_07290"
     CDS             1512..2534
                     /locus_tag="PKI05_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOA52045.1"
                     /translation="MQAFVKTVFLVVAVTFPALIVSAADVVQQGNQVVAGKITQMTRT
                     EVVIETTSGPRTIPVNQIQSISWDGEPATLKNIRMAMQVSQYEDVITALERIKLDTVT
                     RPEIRQDIEFYRAAATAYLALRGTGSIDEAGKLVADFALNYPNNYHVWEATRLVADLL
                     VAKGTVDKAVEYYASLSQAPWPEYKAQAQTAIGWAYLGAGKIDEAEKAFDEVLVIQLS
                     GDDTPKVLATIGKARCLAEKGQTEQAVALIQDLLKRPDLTENAEVMGRAYVTLGLAYR
                     KANDAKRAIIAFLHTDLIYFRHSPSHIEALQNLLELWPQVGHPERAAEVAEVLKKQYG
                     REIGTK"
     gene            2746..>3560
                     /locus_tag="PKI05_07295"
     CDS             2746..>3560
                     /locus_tag="PKI05_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOA52046.1"
                     /translation="MRQGLFSTWTGAVLGSFLSLFVLLAVVPQLPGWLQASATAEEAA
                     DDQLDEPAPAAEAPSEGQVAAPEKSVLAWFFESLGWTYTLAFLTISFSFVALLVMNLV
                     ACRREAVMPQALIEGFETLFNEKRFQEAYELAKNDESFLGQVLSAGLAKLSAGYPQAL
                     EAMQEVGEEQAMRMEHRLSYVGLIGTISPMVGLLGTVDGMVRSFSVIANSTTTPKPSE
                     LARGISTALITTLVGLTIAIPAVIVYGIFRNRIARLVLEVGIVSEGLMSRFSSV"
BASE COUNT          864 a          916 c         1022 g          758 t
ORIGIN      
        1 cgagaggcga gcgaatgtgt gttgacgctc ctcgaaagct ttttcgacct gaacctaatg
       61 aatgatgcta aggaatttct cgagcgcatt cccttagaat cctcgggccg cgcgcgggct
      121 gaacttcgac tgggccaagt gctatggacg caatatgtca aattagcggc cagccaagat
      181 gcgcgcccta gtccggagga gagcgacgcg attctccagg aggcacggcg ctacctggaa
      241 caaggactcc aaaaaatccg gtcgggaggc ggtgaggttg attacctcgt cgagtattcc
      301 gtgttgctct tggcccagtt gcttgtgaat acgggtgaaa cagaccaggc cattaccctg
      361 ttggaagatg cgactctagg ccctctgact ttgatccggc agggacatgc ggctgtggaa
      421 aatgccaagc tccgagaaga ggtcatgaag gtggctctgc gggcgtatgt gggaagtcag
      481 aatctggaca aggcctttga ggttcaagag cagctcgaaa agctcgtcgc gggcagcgga
      541 gatgccgagg cgaccaaacg actaacggcc gtctatgtgg gtctgggacg tcaattggaa
      601 gagcagcttg cccggctccg ctccgaagga aaaaccgcag aaatggagaa agtgctggag
      661 ggcttcacga agtttttgga agcaattcag gaacggtccg cgagtgttga cttccgcgcc
      721 ctcaattggg tggccgagac ctttttgggc ctggcgaaag gattggatcc tggtggtcct
      781 caagtaccag aacaagccaa ggagtattac aaaaaggccg tcgaaacata tatccagctt
      841 atcaaacgtt gtgaagcaga acctagcttt gggccggaag atgcgccgtt gttcctccgc
      901 gttcgcctgg ccgcggcatt acggggatgg cgactgttcg acaaggccat ggaagccatt
      961 ctgttcgtta tcgacaaaca gggcaaagag aatcgtttgg atgcccaact ggaggccgct
     1021 caaatttatc aggaatgggc ggaacagccg gggaaagaac agtactacga atacgccatc
     1081 cgtggtgggc acgagaagaa cggtcggtac ctgatttggg gttggagtgg tatcgcgcga
     1141 cgggtcgcaa gcgactacaa tcgctttctg gacgtttttc accaggcccg ctacaacctg
     1201 gcttattgcc gattcaaatt ggcacaaaaa gcaaccggtg ctaaacgcga tcaactcctt
     1261 aaggaagctg agcttgacat ccaaaggacc taccttctgt accccagcct cggtggaaaa
     1321 gagtggtacg ccaagtacga taagttactg cgaacgattc agcagcttcg cggtgaaaag
     1381 aacccgcaag gtttgaaggc ctttgagagt cggccgcggg caacgccagc atcgtcaaaa
     1441 gccggatgat caggtgaagg taccaacctg gttgtcttag agttgtgaaa taagctaaga
     1501 aggagttttt gatgcaagct tttgtaaaaa ctgtgttcct cgtggtcgct gtaacatttc
     1561 ccgcgctgat cgtttccgcg gcagatgtgg ttcaacaagg gaaccaggtt gtcgctggaa
     1621 aaatcaccca aatgaccagg acagaagtcg tcatcgagac gaccagcggc cctcgtacaa
     1681 tccctgtcaa ccagattcaa agtatctcct gggatggcga gccggcaacc ctgaagaaca
     1741 tccggatggc gatgcaagtc agccagtacg aagacgtgat caccgcactc gagcgtatca
     1801 agctagacac cgtgacccga ccggaaatcc gccaggacat cgagttctat cgagcagcgg
     1861 ccacagctta tttggcgctt cgtggtacgg gatcgattga cgaggcagga aagctcgtgg
     1921 ccgactttgc ccttaactac ccgaacaact accatgtctg ggaggccaca aggctcgtgg
     1981 ccgatttgct ggtcgccaag ggaacggtgg acaaggctgt tgagtactat gctagtctca
     2041 gtcaggcccc ctggccggaa tacaaggccc aggcacaaac agccatcggc tgggcgtatc
     2101 tcggagcagg caagattgac gaagccgaga aggccttcga tgaagtgttg gtcattcagc
     2161 tctccggcga cgatactcca aaggtccttg ccaccatcgg taaggcgcgg tgtctcgctg
     2221 aaaagggaca aacggaacaa gccgtggcct taatccagga cctgttgaag cggcccgacc
     2281 tcacggaaaa cgccgaggtg atgggccggg cttacgtgac gttgggatta gcctaccgca
     2341 aggccaatga tgcaaaacgt gccattattg cctttttgca cacagacctc atttatttcc
     2401 ggcacagtcc atcgcatatc gaagcacttc agaacttgct cgaattgtgg cctcaagtcg
     2461 gtcatcccga gcgggccgcg gaggtggccg aagtccttaa gaagcaatat gggcgggaaa
     2521 ttggcacaaa gtgacgtccc acattgggca agggcaaagg ttttcgcaaa taatccaccc
     2581 tttttgcttg aagaccgcca tttaccacaa gcgaaaagtc agattaaact gaaaaggatg
     2641 ggcaccttaa ttctgagaaa gctagtagct caacacagtc caccaaaagg acatttcctg
     2701 gaaacttagc agaggcgttt ttttcacagt ctaacggagg tactcatgcg gcagggtctg
     2761 ttttcaacct ggaccggagc ggttctcggg tcatttttga gcctgttcgt tctgctagcc
     2821 gttgtgcccc agcttccggg ctggcttcag gcgtcagcga cggccgaaga ggcagccgac
     2881 gaccaactgg acgaaccagc ccccgcggca gaagcccctt cagaaggcca agttgccgcc
     2941 ccggaaaaat ctgtcctcgc atggttcttc gaatcattgg gttggaccta tacgctagcg
     3001 ttcctcacca tctcgttctc cttcgtggca cttttggtca tgaacctcgt ggcttgtcgc
     3061 cgggaggccg tcatgcccca ggcactcatc gagggattcg aaacactgtt caacgaaaag
     3121 cgtttccagg aagcctacga acttgccaag aatgacgagt ctttcctggg acaggttttg
     3181 tcggcgggtt tggcaaagct gtcggcaggt tacccgcagg ccctcgaagc catgcaagag
     3241 gttggcgagg agcaagccat gcggatggaa caccggctca gttacgtggg actgatcggt
     3301 acgattagcc ccatggtagg acttctggga actgtggatg gaatggttcg atcattcagt
     3361 gtgatcgcca atagtaccac gacacccaag ccttccgaac tggctcgcgg tatttcgacg
     3421 gccttgatca cgaccttagt gggtctaacc atcgccattc cggcagtcat cgtttatgga
     3481 attttccgga atcggattgc ccgacttgtg cttgaggtcg gtatcgtcag cgaaggtctg
     3541 atgtcgcggt tctcaagcgt
//
LOCUS       DAOPTR010000325         3557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_21018_length_3557_cov_3.177898, whole genome shotgun sequence.
ACCESSION   DAOPTR010000325 DAOPTR010000000
VERSION     DAOPTR010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21018_length_3557_cov_3.177898"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="PKI05_07300"
     CDS             <1..596
                     /locus_tag="PKI05_07300"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOA52047.1"
                     /translation="YPSFGEHAYDFRAHPEYASGTMKAIWDNIRAVDLLESLPEVDRD
                     RIAVIGHSLGGHNALFTAAFEQRIRAVICSCGFTGFQDYYKGKIAPWAQDRYMPRIST
                     VYGNDPEKVPFDFYEVLAALAPRPVFVNAPLRDANFDCDGVKKILIAIEPVYALHNVR
                     DRIKAVHPDCEHSFPAEIRQAAYQWLEEQFGLAKSTN"
     gene            960..2813
                     /locus_tag="PKI05_07305"
     CDS             960..2813
                     /locus_tag="PKI05_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52048.1"
                     /translation="MGQAEEQYLGPWQVRATADGSRVLVLCKDAAKIAIIDPNTQQIV
                     GWIATPAQPSDFVISPDGQTLFVSAGVADGIVVVLDNSGKRLKEFRVGHSPCGLSLSP
                     DGRFLFVCNRFKNDVTVIDLLKGQTIACPKVIREPVCSAITPDGTKLFVANLLPYDRC
                     DSYDVAASVTCINTQSFETTHIRLLNGSTDVHGLTVSPDGRWVFVVHVLARYQMPTTQ
                     LERGWMNTNALTIIDAAALKRVNTVLLDDVDLGAALPWSVVCSRDGSTVLVSHASTDE
                     VTVVNVPGVLEKLASLPATQEEAKGLGRIYDGSQTSLSAVDVPNDLSFLVGLKERMPL
                     YGTGPYDYLASQNEKIIKGARGLAAVGNKVFVATYFGDCVTVIDLSASKYRRLSYIAL
                     GPTPKLTQERLGEMHFHDASLCFQHWQSCATCHPDGRIDGLNWDLLNDGIGTPKNNKS
                     LLFAHVTPPSMWEGVRATAEDAVRSGIRHIQFAVRPEEDAQAIDAYLKALKPVPSPYL
                     VNGELSESAKRGRELFFSDRLGCRHCHPEPLYTDLKMHNVKSRGKLDRRDEFDTPTLI
                     EIWRTAPYLHDGRYLTLEELFREGQHGQTVGDVAGLSDTELHDLCEFLLSL"
     gene            complement(2965..>3557)
                     /locus_tag="PKI05_07310"
     CDS             complement(2965..>3557)
                     /locus_tag="PKI05_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOA52049.1"
                     /translation="EPRTGSPLPGVIRISWLRWWNFGRALLHALRQGSVSPWNLPPTA
                     SEEERLLISLKGTALRKNDSAFKHIAIAAPTQESFTWLRQAVLPLTSCVSWVHRAIGR
                     NQPGTPPDLLIIDIGTDGCTIEDSLNVLRANLWPDLSPAVPCILLMNFPRWQQVWPLL
                     ARGTTRVISKPVFLPELEWVLAELAQTRDVTCRSPR"
BASE COUNT          808 a          889 c         1028 g          832 t
ORIGIN      
        1 actatccctc ttttggcgaa catgcttatg atttcagggc tcacccggaa tacgccagcg
       61 gaaccatgaa ggcgatttgg gacaacatcc gggcggttga ccttttggaa tctcttcccg
      121 aagtagatcg tgatcgaatc gccgtgattg ggcattctct gggcggacac aatgccttat
      181 tcaccgccgc atttgagcaa cggattcggg ctgtgatttg cagttgcggt ttcacaggct
      241 ttcaggatta ctacaaaggg aagatcgcac cgtgggcaca ggaccgttat atgccgcgga
      301 ttagcaccgt ctatggaaat gatccggaaa aggttccttt tgacttctac gaggtgttgg
      361 ctgccttggc cccgcggccc gtatttgtca atgctccttt gcgcgatgcc aacttcgatt
      421 gcgatggggt gaagaagatt cttatcgcga tagaaccagt ctatgcactc cataatgtcc
      481 gcgatcgaat aaaagccgtg caccctgatt gcgaacacag ctttccggcg gagatccgcc
      541 aagcagccta ccagtggttg gaagaacaat ttggcctggc gaagtccaca aattaggcca
      601 gagggggcgg ccgcgtcata gctgggagca aacttggtca tcgacaaggg tactcccggc
      661 gactatactc tgtggtacgt tgcgtgaccg ggggagctgc aaacttcttg gggtgtttcc
      721 aaaggtaatg catagggcag aagcccacgg ggaagccgca caaatgagga gtggaattaa
      781 tagcagggcg cccttcaacg taaacagtga agattgtctg gattttggcg aataagggaa
      841 gacgtatgct cgcaaagaat gatctctcgt accctgttat acgaagatta tcaagtgagg
      901 ttgtgcgtcg tgtaggactg ttgtttgtat gggttatcct tttccctttc gcagggagca
      961 tgggccaggc ggaggaacaa tatcttggcc cctggcaagt gcgggcgacg gccgacggat
     1021 cacgggtcct cgtgttgtgc aaagacgccg ccaagattgc gatcatcgac ccgaatacgc
     1081 aacagatcgt gggttggatt gcgacaccgg cgcagccaag tgattttgtg atcagtcccg
     1141 atggtcaaac cctgtttgtt tccgcgggag tggctgacgg aatcgttgtg gttttggaca
     1201 attccggcaa gcgtttgaaa gagtttcgcg ttggtcactc tccttgtggg ctctcgttgt
     1261 caccggatgg tcgcttcctg tttgtctgca accgatttaa gaacgacgtc acggtcatcg
     1321 acctgctgaa agggcaaacg atcgcttgcc ccaaggtgat tcgagaaccg gtttgcagtg
     1381 cgattactcc cgatggtacg aaactcttcg tggcgaatct cctgccatat gaccgctgcg
     1441 atagctatga tgtggcagct tctgtcacgt gcatcaatac gcaatcattt gaaacgaccc
     1501 acattcgact cctcaatggt tcaaccgatg tgcacggttt gacggtttca cccgatggca
     1561 ggtgggtctt cgtcgttcat gtgcttgctc ggtatcaaat gccgacgacg caactcgaac
     1621 ggggatggat gaacactaac gcgctgacga tcattgatgc agccgcactg aaacgagtga
     1681 acaccgtcct tttggatgat gtggatttgg gagcagcact gccctggtcg gtggtgtgtt
     1741 cacgggatgg ctctacggtc cttgtgtcgc atgcgtccac agatgaggta acggttgtta
     1801 atgtgccagg ggtattggaa aaacttgcct cactgccggc gacacaggaa gaagccaagg
     1861 gtttggggcg gatttacgac ggctctcaaa cctccctcag tgcggtggat gttccaaatg
     1921 acttatcctt ccttgtgggt cttaaagaac gtatgccact ttatggaaca ggaccgtatg
     1981 attatctggc cagtcagaat gaaaagatta tcaagggggc aagaggcctg gccgcagtgg
     2041 gtaataaggt gtttgttgcg acctatttcg gggactgtgt gacggtcatc gatttgtctg
     2101 cttcaaaata tcgtcgttta agctacatcg cgcttggacc tacgccaaaa ctgacccaag
     2161 agcggttggg cgaaatgcac tttcacgatg ccagcctgtg ctttcagcac tggcaaagct
     2221 gcgcgacatg ccatcccgac ggtcgaattg acggcttaaa ttgggacttg ctcaacgatg
     2281 ggatcggaac accaaagaat aacaagagcc tgctatttgc ccatgttacg cccccgtcaa
     2341 tgtgggaagg tgtgcgggca acggcggaag atgccgtgcg ctcgggaatc cgtcacattc
     2401 agtttgccgt tcgtcccgag gaggatgccc aggccatcga cgcttatctc aaggctctca
     2461 aacccgtacc cagtccgtat cttgttaacg gtgaactcag tgaatcggcg aaacggggac
     2521 gtgaattatt cttcagtgac cgcctaggct gtcggcattg ccatcccgaa ccgctttaca
     2581 ccgacttgaa aatgcacaat gtgaaaagcc gggggaaatt ggatcgccgc gatgagttcg
     2641 acacacccac gttgatcgag atttggcgga cggcccctta cctccatgac ggtcgttatc
     2701 tgacactgga ggagttgttc cgggaaggac aacatggtca gacggtgggc gatgtggcgg
     2761 ggctttcgga taccgaatta cacgatttgt gtgaatttct gctgtcacta tgacgctgag
     2821 caagtatgtc caggtagccg gcaaccgcct ctgctgtcag agcatggcat gcgtcgtggg
     2881 atcaagacgc cgcggtgtcg gggcgagggc gattgactcc cagccatagt tagcagattg
     2941 gctatttgag acgaagttgt cccgtcagcg tggagaccga catgtgacgt ctcgcgtctg
     3001 agcgagttct gccaggaccc attcaagttc cggcaagaaa acgggcttgc tgataaccct
     3061 tgtcgtgcct cgggcaagaa gcggccaaac ttgctgccag cggggaaaat tcattagcaa
     3121 aatgcatggc accgcgggtg aaagatctgg ccaaagattt gcccgcaaga cgttgagcga
     3181 gtcttcgatc gtgcatccgt cagtcccgat gtcgataatc aacagatccg gcggggtgcc
     3241 gggctgattc cttccgattg cgcggtgcac ccaggatacg caagacgtca atggcaaaac
     3301 ggcttgtcgc agccaggtga agctctcttg tgtgggagcg gcaatagcga tgtgcttaaa
     3361 agcggaatcg tttttccgca aagctgtgcc tttcaaacta atcagcaatc gctcctcttc
     3421 tgaggccgtg ggcggtaggt tccacgggga gacactacct tgccgcaacg catgcagcag
     3481 cgctcgaccg aagttccacc agcgaagcca agagatgcga atgacccccg gtagcggtga
     3541 acccgtccga ggttcgc
//
LOCUS       DAOPTR010000326         3548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_21087_length_3548_cov_3.461494, whole genome shotgun sequence.
ACCESSION   DAOPTR010000326 DAOPTR010000000
VERSION     DAOPTR010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21087_length_3548_cov_3.461494"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="PKI05_07315"
     CDS             <1..1183
                     /locus_tag="PKI05_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BREX system Lon protease-like protein BrxL"
                     /protein_id="HOA52050.1"
                     /translation="MPKEVVTTLKTYCESGTFARGKDALTGMASVAMFGNTNQSVDVM
                     VRSSHLFQPMPAVIREDMAFLDRLHFYVPGWEIPKMRVEFFTDHYGLVVDYFAEALRE
                     LRKHNFTEAIDRFFSLGSHLNARDVKAVRKTVSGLIKIIYPHGEFSRNELAELLELAM
                     EGRRRVKEQLKKLGAFEYHQTSFSYMDNETREERFVGVPEQGGQDIISPDPLAPGSVY
                     TASVDDQGRVGLYRLEVSCSSGTGKLRVAGGIDGTMRESIQRAFAYLIAQKSKIGLEQ
                     QLDSTDFHVEGIDLLGNHVPCEAGIGFIVAVYSALKKQSVLAGLLILGDLSIQGNIKS
                     LRSLVEALQVGMDNGARRALIPLENKRNFLEVPGDIVERVDPIFFSDPMTAAVKALGI
                     A"
     gene            1617..>3548
                     /locus_tag="PKI05_07320"
     CDS             1617..>3548
                     /locus_tag="PKI05_07320"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOA52051.1"
                     /translation="MKNRYEKHTRWARGLLAGVLWLQMCVGFAEAEAGELVGKVTDPQ
                     GKPIAGARLYLVDTIRGIERSSITDPTGHYRIASLSPGEYLWQSFCVGYRTLRGRLSL
                     PESNQPLTHDLELPSAQEYRLENEKLLLVIDENGTVRELRNKQVDASVNYVSGQAPGF
                     WHIVFRRGECWENVVLAEAQQYQIEKPAQNRLVLRVDRLRFRQETFPVTIQFSIQLHE
                     DELSWAATVENRAELEITEFYFPELGGVRSLGGKSGKEYLYWPNGLGERMDGFASRTL
                     RMTYPWPASMAWFTVCNGQEGIYCGAHDDSFLSSELQVGPNPLQANEPTLGFVKFPFI
                     RPGETWTSKPYVVACYKGTWHTAADKYRRWMHTWRKTLEKPAWVQRTTGMFLVILKQQ
                     YGDIMWHYDEIPLLYEEAQRNGINMVALFGWTQGGHDNQYPQFLPDPKMGGAEVLRRA
                     LQQVRASGGHVVCYMQGRLLDAQSEYVQSPEGQKVVARNLWGGPYVEEYNKYYHSSLL
                     RLYSRKLQMIANPIHPEWRTTLLEKAQEVIQHGPNGILFDQIGGLYAYPAFDTPPEIK
                     PTLAFPEGQQKLLAFLRTQLKQKQSDFGLLSEHLTDVYSQYVDMLHGCGAGYAPGPTS
                     FPAMARYVLPDVILTSRNPM"
BASE COUNT          779 a          935 c         1033 g          801 t
ORIGIN      
        1 aatgcccaag gaggtggtaa cgaccctcaa aacctactgt gagtccggga cgtttgcccg
       61 aggtaaggac gcgttgacgg gcatggcctc cgtagcgatg tttggaaata ccaatcagtc
      121 ggtagatgtc atggtccgct cttcgcatct gttccagccg atgccggccg tgattcgtga
      181 ggacatggcc ttcctggatc ggttgcactt ctacgtacct gggtgggaaa ttccgaagat
      241 gcgagtggag ttctttacgg accactatgg attagtcgtt gactacttcg ccgaggccct
      301 tcgagaactg cgcaagcaca acttcacgga ggccatcgac cggttcttct ccctgggttc
      361 acacctgaac gcacgggacg tcaaggcggt gcgaaagact gtgtcgggat tgataaagat
      421 catctacccc cacggcgagt tttcacggaa tgagctggct gagctccttg aactggccat
      481 ggagggaagg cgtcgcgtca aggaacagct caagaagctc ggggcatttg agtaccacca
      541 gacgtcgttc tcctacatgg acaacgagac gcgggaggag cgattcgtcg gtgtaccgga
      601 gcagggcgga caggacatta tttcgcctga tcctctcgct cctggctcgg tttacacagc
      661 ctccgtggac gaccagggaa gagtcggttt gtaccgtctc gaggtgagct gttcatctgg
      721 cacggggaag cttcgagttg ctgggggcat cgacggcacg atgagggaat cgatccagcg
      781 agcctttgcg tatctgatcg cgcagaaaag caagataggc ctggagcagc aactggactc
      841 caccgatttc cacgtcgagg gcatcgacct actcggcaat catgttccat gcgaggcagg
      901 gattgggttc attgtggctg tgtactcggc cctcaagaag cagtcggtgc tggcgggact
      961 gctgattctg ggtgacctca gcattcaggg gaacatcaag tcgctgcggt cgcttgtgga
     1021 agctcttcag gtgggaatgg acaatggagc tcgtcgcgca ctcatcccgt tggagaacaa
     1081 gcgaaacttc ttagaggtac ccggtgacat cgtcgaacgc gttgacccaa tcttcttctc
     1141 ggatcctatg actgcggccg tgaaggccct tggcatagca tgatgataga atgcaacatt
     1201 cgatagccct gtgtagcgag agctcgcgcg ggccagtccg ccggccttgg aatccttatt
     1261 gaccgtccgg accaagaagg ggctggtgta ggccacaagc gagaatctcc cgatagaaga
     1321 acccggcgtc caaaagcaac aacatgaacg cccgccgcgc ctgcttggcc tggcgaaaaa
     1381 tgtggagtgc ggtgttgcga tcctcggccg aaaggtggat actaccatcc gtggcttggt
     1441 ctccagggtg ggttgtggct ttacaataac cgtctacctt agagccaagc ctgttgctat
     1501 ttccaggtca gttttcggcc cccctgctgg ggcgctatgc tcccggccag gaccgatttt
     1561 gttttccagg acttttggag gaaaccttgg ttccaaacct ctgtgtggag gtcggcatga
     1621 aaaacaggta tgaaaaacac acgagatggg ctcgtggact cctcgccgga gtgctttggc
     1681 tgcaaatgtg cgttggtttt gccgaggcgg aagccggaga gttggtcgga aaagttactg
     1741 atcctcaggg caagccgatc gccggggccc ggctttactt ggtggacaca attcgtggga
     1801 tcgagcgatc gtctattacc gatccaacgg gacactatcg aattgcctcc ctttcccccg
     1861 gggaatacct ttggcaaagt ttttgcgtag ggtatcgtac tctgcgtggt cggctctccc
     1921 tgccagaatc gaaccaaccc ctgacccacg atttggagct tccgtcggcc caagagtatc
     1981 ggctcgaaaa cgagaagctt ctgcttgtga tcgacgaaaa cggcacggta cgtgaacttc
     2041 gcaacaagca ggtggatgcg agtgtcaatt atgtcagcgg ccaagctccc ggattttggc
     2101 acatcgtgtt tcgacgggga gagtgttggg agaatgtggt cttggccgag gcccaacagt
     2161 atcagatcga gaagccggct cagaatcggt tggtccttcg ggtggaccgg ttgcgcttca
     2221 ggcaggagac atttccagtg accattcagt ttagcatcca attgcacgag gatgaactga
     2281 gttgggccgc cacagtggag aatcgtgcag agctggaaat tacggagttt tattttcccg
     2341 agttgggcgg cgtccggtct ttgggtggaa agagtgggaa ggagtatctt tattggccga
     2401 atggactagg ggaacggatg gacggattcg cttcgcgtac tctgcgaatg acctatcctt
     2461 ggcctgcttc catggcttgg tttacagtgt gcaacgggca ggagggaatc tattgcgggg
     2521 cccacgacga cagtttttta agtagtgaac ttcaagtggg gccgaatcct cttcaggcca
     2581 atgagcccac tttggggttt gtgaagtttc cgtttatccg acctggtgaa acctggacca
     2641 gcaaacccta tgtcgtcgct tgttacaagg ggacttggca taccgccgcg gacaagtatc
     2701 gccgttggat gcacacctgg cgcaaaacgc tcgaaaaacc tgcctgggtc caacgcacga
     2761 ccggcatgtt tctggtcatt ctcaaacagc aatatggcga catcatgtgg cactatgacg
     2821 aaatccctct tttatatgaa gaggctcagc gcaatgggat caatatggtg gcccttttcg
     2881 ggtggaccca aggcggccat gataaccaat atcctcagtt tctgcccgat cccaaaatgg
     2941 gaggggcaga ggtcttgcgt cgcgcgcttc agcaggtgcg agccagcggc ggccatgtcg
     3001 tctgctatat gcagggtcgg ctcctggacg cgcaaagcga gtatgtccaa agcccagagg
     3061 gtcaaaaagt ggtggcccgc aatctctggg gcggtcccta tgtcgaggag tataataagt
     3121 actaccatag tagtcttctg cggctttact ccagaaagct ccagatgatt gccaatccga
     3181 tccatccgga atggcggacc acactcctgg aaaaagccca agaggtcatc caacacggcc
     3241 cgaacggaat ccttttcgat caaatcggcg gtctgtacgc ctatcccgcc ttcgacaccc
     3301 cgccggagat caaacctact ctcgcctttc ccgaaggaca acaaaagttg ctcgccttcc
     3361 ttcgtaccca gctcaagcag aagcaatcgg atttcggtct gctctccgaa cacctgaccg
     3421 atgtctattc gcaatatgtc gatatgcttc atggatgtgg cgcggggtac gcacctggac
     3481 cgacgtcttt cccagcaatg gcgcgatacg tcttgccaga tgtcatcctt acctctcgca
     3541 accccatg
//
LOCUS       DAOPTR010000327         3537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_21167_length_3537_cov_2.660827, whole genome shotgun sequence.
ACCESSION   DAOPTR010000327 DAOPTR010000000
VERSION     DAOPTR010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21167_length_3537_cov_2.660827"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PKI05_07325"
     CDS             complement(<1..388)
                     /locus_tag="PKI05_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52052.1"
                     /translation="MRSTPIVAITWVVAWLAPILIVFVSYGQEPQEELLWPQGAPLAK
                     GDRPEDKPRLIIYLPSSDKAVGTGVVVCPGGGYGALAMDHEGHQIAQWFNSFGVAAFI
                     LDYRHRNKGYMHPAPMLDVQRAIRLVR"
     gene            625..1479
                     /locus_tag="PKI05_07330"
     CDS             625..1479
                     /locus_tag="PKI05_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52053.1"
                     /translation="MKSLQQNVAREKLLASFAAAVLMVFLGTTQAFANGLPAGIAFFP
                     GMAPIDPVCGPLLTVAIAIIERPFFARAGLTPWALIHSLRANFASYLLGIVVTLIYVA
                     TVDAVIRAGFLNQDVFILLLPIVLISVTIWFEERLAVRVLAPTRKLRTRWIIAANVVS
                     NLALFAIMMCFGWIFSILGFHVYELIFWLEQWQAVFIVAYLLIFFGGISLALGVPLWK
                     LLPQSLQRKWGIPLSVPETGFDNQVHETAPFEKASVASQAVSGEEIGNRVAEQPSPKA
                     QEPVRAGP"
     gene            1649..3289
                     /gene="serA"
                     /locus_tag="PKI05_07335"
     CDS             1649..3289
                     /gene="serA"
                     /locus_tag="PKI05_07335"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOA52054.1"
                     /translation="MVKVVVLDPVSPEGLKLLEAAGTIEYEVRTGLKGEDLRKTLAEF
                     DGAICRSGVKITADALEGNKRLRVIVRAGVGTDNIDTAAATRQGIVVMNTPGGNTIAT
                     AEHTMALMLALSRNVFPAYKSLLEGRWERHQFVGTELCGKTLGLIGLGRVGQAVAERA
                     LAFKMRVLGYDPFLPEHRARELGVQMCHSIDEMLPEVDYLSVHTPLNDQTRNLIDRPQ
                     IQKMKKGVRLINCARGGIYNEEALLEGLQSGHIAGVALDVFTKEPCTASPLFGQPGVL
                     CTPHLGASTEEAQTNVAIEACQLLVDFFTTGAVRNSVNMAPLDPKTLEGLRGFLDLTY
                     RLGLLTAQLDREAPTRCRIRYQGEVANRDTRLLSAAFAAGLLQHALEQAVNLVNAPVL
                     MKQRGIEVTEERTTDIGDFASQITVEVFRNDKSVVAGGTLFGKSLPRLIQKGPYRLES
                     PLEGTMLIMYHRDVPGVIGRVGGICGRHNVNIAFLSLGRAENKPGGEAIGILALDNYP
                     PAEAIGEILKLDPIHRAIVVSLPPRGELPSWLGVGLPQ"
BASE COUNT          733 a          910 c         1045 g          849 t
ORIGIN      
        1 cgcgaacgag ccgaatggcc cgctggacgt ccagcattgg ggcagggtgc atgtaaccct
       61 tatttcggtg acgatagtcc aagatgaatg ccgccacgcc aaaggaattg aaccattggg
      121 cgatctggtg cccctcgtgg tccatggcca gcgccccgta acccccgccg ggacaaacca
      181 ccacacccgt tcccacggcc ttgtcagaag acggcaggta aatgattaac cgcggcttgt
      241 cctcgggacg gtcgcctttg gccaaagggg cgccctgggg ccagagcaat tcttcctgag
      301 gctcttggcc gtatgataca aagacgataa gaatcggggc caaccatgcc acaacccaag
      361 tgatggcaac aataggtgtg cttctcatga acctgtttct ccttgctgct tgccaatgga
      421 acttgcactt gaactgcgtg atcaacaaat aacgtatcac cgtccgttcc agaatgccac
      481 accctaggat tgtccgaggt gaagtcactc tcgcaaaaaa acctattcga ctgtaaacgt
      541 cgcgaaggta tgtgataaaa tagcgggcgg tgacttcgat cgtggcaaaa ttgcacttca
      601 tgccgactag cgcggagatg gggaatgaag tctcttcagc agaatgtggc ccgcgagaaa
      661 ctcttggcta gttttgcggc cgcagttttg atggtttttc ttggaacaac gcaggccttt
      721 gccaacggcc taccggcggg gattgctttc tttccgggga tggcgccgat tgatccggtg
      781 tgtgggcctt tgctgaccgt cgcgattgcg atcatcgagc ggccattttt cgctcgagca
      841 gggttgactc cttgggcact tattcactcc ctgcgggcga attttgccag ctatttgctt
      901 ggcattgtgg tcaccctgat ttatgttgcg acggtcgacg cggtcatacg tgcgggcttc
      961 ctcaatcagg atgtcttcat tcttttgctt cccatagtgt tgattagcgt cactatttgg
     1021 ttcgaagaaa gactcgcggt tcgcgtcttg gcaccgacac gcaaactccg cacgcgctgg
     1081 atcattgcgg ccaatgtggt aagcaacctt gctttattcg cgatcatgat gtgttttggc
     1141 tggatctttt caatcttagg gtttcacgtt tatgaactta tcttttggct agaacaatgg
     1201 caggctgtat ttatcgtcgc gtatcttttg attttcttcg ggggcatttc cttggccttg
     1261 ggcgttcccc tttggaagct tttacctcag agccttcagc gaaaatgggg tatccccctc
     1321 tctgtgccgg agacggggtt tgacaatcag gtgcacgaaa ccgcaccttt tgaaaaggcc
     1381 tctgtggctt ctcaggcggt gtccggcgaa gagattggaa atcgcgttgc ggagcagcca
     1441 tcccctaaag cgcaggagcc tgttcgcgcg ggtccttgac gccccgagag actcgtcgta
     1501 aaaggaatgg gttatgcccg ggatgaggag gacaccgttg gtccgcgtgc gcggtactgc
     1561 gacgccgtaa cgagtttccg tgcgccgctt ggtgccttca cggttatggg ctcctttttg
     1621 cattgtgttt gaaattgagg gtctgaccat ggtaaaggtg gttgttttgg atccggtttc
     1681 gcccgagggt ctcaagctat tggaagcggc cggtacgatc gaatacgagg taagaacggg
     1741 tcttaagggc gaagaccttc gcaagacgct ggcggaattc gacggcgcga tctgccgaag
     1801 tggtgtcaag attacggcgg atgctttgga ggggaacaag cgtctgaggg tgattgtgcg
     1861 ggcgggtgtc gggacggata acatcgatac cgcggcggct acgcgacagg gtatcgttgt
     1921 catgaacacg cccggcggga acacgatcgc cacggccgag catacgatgg ccctcatgct
     1981 ggccttgtcc cgtaatgtct ttcccgctta caagagtcta ttggaaggga gatgggagcg
     2041 gcatcagttt gtgggtacgg agttgtgcgg aaaaacgctt ggtctgatcg gactaggacg
     2101 tgtcgggcag gccgttgccg agcgggcctt ggccttcaag atgcgggtcc tcggctacga
     2161 cccgttcctt ccagaacatc gtgcccgcga attgggtgta cagatgtgcc acagtatcga
     2221 tgaaatgctg ccagaggttg actacctcag cgttcataca ccgctaaatg atcagacgcg
     2281 aaatctcatt gaccgcccgc aaatccagaa gatgaaaaaa ggcgtgcgtc tcattaactg
     2341 cgcacggggt ggcatttaca acgaagaggc actcctggaa ggtttacagt cgggacatat
     2401 cgccggtgtg gcgctcgacg tgttcaccaa agagccgtgc acagcaagtc cattgtttgg
     2461 gcagcccggg gtgctctgca cgccgcacct gggagccagc accgaagagg ctcagacgaa
     2521 cgttgccatc gaggcctgcc agcttttggt ggacttcttc accacggggg cggttcgcaa
     2581 ttcggtcaat atggcccccc tcgatccgaa gactctggaa gggttgcgcg gctttttgga
     2641 ccttacttac cgactgggct tactcacggc tcaactagat cgggaagcgc cgacgcgttg
     2701 ccgtattcgt tatcaagggg aagttgcaaa tcgggatacg cgattgttat cggcggcgtt
     2761 tgcggcggga ctcctccagc atgccttgga acaggctgtt aatctggtta atgcccccgt
     2821 gctgatgaag cagcggggta ttgaggtgac tgaggaacgg acgactgaca tcggtgattt
     2881 cgcatcgcag attacggtgg aggtcttccg taatgacaag tcagtcgttg ccggtgggac
     2941 gctgtttggg aagtcattgc cacggttgat ccaaaaaggg ccctatcgac tagaaagccc
     3001 cctcgaaggg acaatgctca ttatgtacca tcgggacgtt cccggcgtca ttgggcgagt
     3061 tggcggaatt tgcggacgcc ataacgtcaa tatcgccttt ctttcgctgg gacgtgccga
     3121 aaacaagccg ggcggcgagg ctatcggaat ccttgccctc gataactacc cacctgctga
     3181 ggccatcgga gaaatcctca aacttgaccc aatccatagg gctattgtgg tcagcctacc
     3241 gccgagggga gagcttccct catggttggg ggttggccta ccgcagtgac gtatcggtat
     3301 aaacgcgtag tactgttggc gacgagggag ccgagtccgc ttcctcgtcg ttttttctgg
     3361 acgtttccta tggtggttcc gtgtaagcta ttcttgctat agcacgtgta tctttgggca
     3421 gagaacgctg gatgagcgga cacatgctcg ccgattaccc ttacatggag cgatctttac
     3481 ccgcatcgtg ctagcgatga cacggactgc tgccgaggtt aaaagaaacc ttgcgtg
//
LOCUS       DAOPTR010000328         3533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_21186_length_3533_cov_3.500000, whole genome shotgun sequence.
ACCESSION   DAOPTR010000328 DAOPTR010000000
VERSION     DAOPTR010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21186_length_3533_cov_3.500000"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PKI05_07340"
     CDS             <1..233
                     /locus_tag="PKI05_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52055.1"
                     /translation="LTGLLPIGLLQVYHAYANGFWWGRSAAFYEMPLIQALGNFRIIP
                     DTIIIVVGALPLLYFLLRTFPRLRTAQGSEQG"
     gene            772..1917
                     /locus_tag="PKI05_07345"
     CDS             772..1917
                     /locus_tag="PKI05_07345"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOA52056.1"
                     /translation="MQLWHSLSQLRWILLSCLGALSVTVLLEPSETCPGSAMARAEAT
                     PRAEPAVELSTDYRDEGPGYICEIRNGRTFYGDTSRRGVPFAKDPSVVRFGDRYLMYY
                     SIPPFAKDRPNNGWAVGIAESRDLGRWKKVGEILPMQECDAKGLAAPCAKVLGGRLHL
                     FYQTYGNGPRDAICVAVSDDGLRFTPHPSNPIFRPSGQWTVGRAIDADVISFKGRLFL
                     AYATRDPSMKIQMLGMATADPESDLGPTAWHQCGEGPILRPELPWEQDCIEAPSMVVR
                     DDKLIMFYAGAYNNRPQQIGVAASDDGLHWKRLFTEPLIPVGPPDAWNASESGHPGVF
                     VDEDGQTYVFYQGNNDQGRTWWITWDRVTWQGTIPYVIPAERQVSQK"
     gene            complement(2029..>3533)
                     /locus_tag="PKI05_07350"
     CDS             complement(2029..>3533)
                     /locus_tag="PKI05_07350"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOA52057.1"
                     /translation="SGVHNKRSVVIMWRTLLYIPTHVGQMPVFGFGLLFWAWVILTIG
                     LVWWQVRRHGWTNELWSTVVLYGIVGVLIVLVLPRVTVDIPGLVDTKSQPVRGLPIRG
                     YGSMLLLGVASAVGLAIYRAKRRGLSVEWVSNLALWCIIPGMIGARAFHVIEYWPIHY
                     GPLWKTAGPIAGLMAIANVAQGGLVVYGSVIGGLLGIALYAWQQRTRFLSILDLLTPC
                     FLIGLAFGRIGCFLNGCCFGGVCELPWTVRFPAGSFAYLSQAEQGQLALYGIKFKEDP
                     HHPQRLPVILEVAAQSAAARAGVKPGRHIAAINGLPLAGTEGESPTSRLVVQLIALYG
                     DPVVLEGGRGCVCSHGPNVIDISKISRSGVSSNATHLPASHSIMIRIDTAEGDHYQWE
                     TTEGLPARSLAVHPSQLYSAANAAILCLFVLAYEPFRKREGELFAWFLILYPVTRFLL
                     EIIRADEPGSYGGLTIAQVVSLGLLVCGIATWIYLWRFTPGEQPNQAKSP"
BASE COUNT          765 a          987 c         1091 g          690 t
ORIGIN      
        1 tcctcaccgg gttactgcct atcggtttgc tccaggttta tcatgcctat gcgaacggct
       61 tttggtgggg acgaagtgcc gccttttacg agatgccctt gatccaggcc ctcggtaact
      121 tccgcatcat ccccgacaca atcattatcg ttgtcggtgc cctgccgttg ctgtactttt
      181 tgctgcggac gtttccacgg ttacgcacgg ctcaggggtc ggagcagggt tgatcgcccg
      241 cggatagaga ccaccttctt gggaaaactc agagccagtt ggccacgatg gtcgatgggc
      301 ctccagcagg gggccatcaa ttgctagggg caaccccccc gtcgttgccc attgttgaac
      361 ccggaaggac gtgcaccgtc gtctccaagg tggctcagtt ataatgatct gctacggcgc
      421 aaccggtgtg atccgaggca catcgaatct tcgcgtgccc cgtggtgttt gattggcacg
      481 gttgcgaggg gtgccagttc ggccttaggg tggcaccggg cgaggcggtc aggagagggg
      541 ccgcaatcgt gacggccaca gtcggtcgta agagcctatc ccaaaaagtc cccctctccc
      601 tccgggagag ggttggggtg agggcaggga gggacgcggt cccccgcgtc cgtcatttag
      661 agcctatccc aaaaggtccc cctctcccac cgaacgggcg agggggagat tatgggatgg
      721 gttctaagtg tgtgaccacc caccagccgt gaatttcatt gtgaggagaa tatgcaactt
      781 tggcattcat tgtctcagct tcgttggatc ctgctgagct gcttgggtgc gttaagcgtt
      841 acagtgctgc ttgagccttc agaaacttgt ccgggtagcg ccatggctag ggccgaggca
      901 acacccaggg ccgaaccggc ggtggaatta tcgaccgatt accgcgacga ggggccggga
      961 tatatctgcg agatccggaa cggtcggacg ttctacggag atacgtcccg gcggggagtg
     1021 ccttttgcca aggatccgtc tgtggtgcga tttggggacc ggtatttaat gtattattcg
     1081 atcccgccgt tcgctaagga ccgtcctaat aacggctggg ccgtgggaat tgccgaaagc
     1141 cgcgatcttg gaaggtggaa gaaggtgggc gagatcctgc ccatgcagga atgcgatgct
     1201 aagggcttgg cggcaccgtg cgcgaaggtc ttgggcggtc ggctgcatct cttctatcag
     1261 acgtatggga acggcccgcg ggacgcgatc tgcgttgccg tgtccgatga tggcctgcga
     1321 tttacgccgc accccagcaa tccgatcttt cgccctagcg gccagtggac tgttggccgg
     1381 gcgatcgacg ccgatgtgat ctcgtttaag gggcgtctct ttttggccta tgccacgcgg
     1441 gacccctcga tgaagattca aatgctgggt atggcgacgg ccgatcccga atcggatctt
     1501 gggcccaccg cttggcacca atgcggtgag ggaccgatcc tccgaccaga gcttccctgg
     1561 gaacaagatt gcatcgaggc gcccagcatg gtggtccgag atgacaagct catcatgttt
     1621 tatgccgggg cgtacaacaa ccggccgcag caaatcgggg tggcggccag cgacgatggt
     1681 ctccactgga aacgattatt caccgagccg ttgattccgg tcggtccgcc cgacgcgtgg
     1741 aatgcttcag agtctggcca tccgggtgtc tttgttgacg aggatgggca aacgtatgtg
     1801 ttctatcagg gcaataatga tcaggggcgg acgtggtgga ttacctggga tcgcgtcacg
     1861 tggcagggga cgatccccta tgtgataccg gcggagcgac aggtctccca aaagtgagtg
     1921 tctaaaccgg gatgtgcact ccaaaccaca gcacgcgatg gcgcaatacg aaggaacgtc
     1981 gcactggctc gatcagggcc aggacgcgat cgccttagcg aagcgacatt aaggactctt
     2041 ggcctggttc ggttgctctc ctggtgtgaa tcgccagaga tagatccacg tggcaatccc
     2101 gcacacaagc agtcccaggc tcacgacctg ggcaatcgtc aaaccaccgt agctgcccgg
     2161 ctcatcagcc cgaatgattt ccaaaaggaa tcgggtcacg gggtagagga tcagaaacca
     2221 ggcaaaaagt tcgccctcgc gcttacggaa cggctcgtac gccaggacaa agaggcacaa
     2281 gatggcggca ttggcggcgc tatagagctg gctggggtgg acggccaggc tgcgggcggg
     2341 caggccttcg gtggtttccc actggtagtg atcaccctcg gccgtgtcga tacggatcat
     2401 gatggagtgg gaagctggca aatgggttgc gttcgaggag acaccggagc gcgatatctt
     2461 ggagatgtca atcacatttg gcccatgtga acagacgcag ccgcgtcctc cttccaagac
     2521 gacgggatca ccgtagaggg cgatgagttg cacgacgagg cgtgaggtgg gcgattctcc
     2581 ctccgtccct gccaggggaa ggccgttaat cgccgcgata tggcgacccg gcttcactcc
     2641 cgcccgagcg gcagccgatt gggccgcgac ctccaggatc accggaagtc gctggggatg
     2701 atgagggtct tccttaaact tgatgccata gagggcgagt tgcccctgtt ccgcttgact
     2761 gagataggca aacgatccgg ccgggaagcg aacggtccaa ggaagctcgc acaccccgcc
     2821 aaaacaacat ccattgagaa aacagccaat tcgtccgaaa gccaggccga tcaaaaagca
     2881 tggagtcaaa agatcgagga ttgacaaaaa ccgtgttcgt tgttgccagg cgtaaagggc
     2941 aataccgagc aatccgccaa tcaccgagcc ataaacgacc agtccgcctt gggccacatt
     3001 ggcgatggcc atcaaaccgg cgatgggccc cgcggtcttc cagaggggac cataatggat
     3061 cggccaatat tcaatcacgt ggaaggcccg ggcaccgatc atccccggga tgatgcacca
     3121 cagggccaga ttgctcaccc attccacaga cagaccacga cgtttggcac ggtagatggc
     3181 caatcccacg gcactggcga cgcccagcag aagcatagag ccatagccac ggatgggcag
     3241 gccccgcaca ggttggcttt tggtatcgac aagccctggg atatcgaccg tgactcgcgg
     3301 aagaaccaag acaatgagca cgcccacgat gccgtaaagg accaccgtgc tccacagttc
     3361 gttggtccag ccgtgacgac ggacctgcca ccagacaaga ccgattgtga ggataaccca
     3421 ggcccagaaa agtagcccga acccaaaaac gggcatttgg ccgacgtgag ttggaatgta
     3481 aaggagtgtt cgccacatga tcacgacact ccttttgttg tgaacacccg aaa
//
LOCUS       DAOPTR010000329         3532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_21193_length_3532_cov_3.574346, whole genome shotgun sequence.
ACCESSION   DAOPTR010000329 DAOPTR010000000
VERSION     DAOPTR010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21193_length_3532_cov_3.574346"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="PKI05_07355"
     CDS             <1..733
                     /locus_tag="PKI05_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOA52058.1"
                     /translation="GKQSGAFSAGTYDGAPYILMNYQPDVFDHVFTLAHEAGHSMHSY
                     YSAHHQPYHLYQYTIFVAEVASTFNEQLLHRYLMGQAKSDEERAYYINHEIDEIRGTI
                     FRQTMFAEFEKMIHEHVEKGDALTVETFRTMYRSLLNDYFGPDVKVPDLLELECFRIP
                     HFYNAFYVYKYATGLSAAIALADRVLQGGKNELDAYLAFLSAGCSKDPLDLLRDAGVD
                     MERPDPVAAAMERFDTLVDELEKIL"
     gene            777..1955
                     /locus_tag="PKI05_07360"
     CDS             777..1955
                     /locus_tag="PKI05_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOA52059.1"
                     /translation="MIAQESSVSLSTWDEFRSLMPICGRYVYFDHAAVAPLPGPTRQA
                     IESWLLEATEDGDTRWPEWAQRLEAFRRDIAEFIGALPEEIALVRNTSHGISIVAEGF
                     PWQAGDNVVIPAQEFPANQYPWLALESFGVEVRRVPPKHGVIDLKALEEACDARTRIL
                     SVSWVDYLSGFRIAPAELAALAHRYGAYFFLDAIQGLGVSPIDVHQVEIDFLAADGHK
                     WLLGPEGAGVFYCRAPLWEMLRPTSIGWNSVEHPFEFDRIDPNWKKSAARFEGGSQNI
                     IGFLGLHASLSLLRRYGPAAISRRVLDLTNTICEVLRSLGAILLSRRDEGVASGIVTF
                     SIPDLDVIRLREALLRRRIVLSVRQKALRVSPHAYNNEHDVAAFGEALREAVDECRAS
                     "
     gene            1988..3349
                     /gene="mgtE"
                     /locus_tag="PKI05_07365"
     CDS             1988..3349
                     /gene="mgtE"
                     /locus_tag="PKI05_07365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOA52060.1"
                     /translation="MSNEAHTIAKRIIEALDRRDHEELVRLLQKVPEGELPQRLAQLT
                     EADQRRLLEAIPAEVASSLLQELPESQVTRLLEELAPSQAAAILDHLPSDEQADLLGA
                     LPDEEASKLLEIMNPEEASDARRLLQFAPDTAGGLMITEYLAYTETCTVEDIVEDLRI
                     NAARYRSYDVQYFYTVDAEGRLTGVLRVRDLLLSEPDTQIATIAVRQPVSVATDTSLD
                     KLEHLFAHYQFYGLPVVDRDGRLVGLVRRYDVEEAIAARANRNFLKVVGISGGEELRS
                     MPLRDRIKGRTSWLTVNLILDLIAASVIPFFEETISAVIALVFFLPIISDMGGNTGIQ
                     SIGVTLRELAAGLITPRDAWRTFWRELPVAVINGLVVGFLLAGVALVWRGNPYLGILI
                     GSALTLNTVIAACLGAVLPLGLRRIGIDPAVASGPILTTIADLTGFFMVLGGAAVILP
                     YLT"
BASE COUNT          738 a          898 c         1022 g          874 t
ORIGIN      
        1 tgggaagcag agtggggcat ttagcgctgg gacgtacgat ggggcaccct atatcctgat
       61 gaactatcaa cccgatgtgt ttgatcatgt ctttacctta gctcacgaag ccgggcattc
      121 gatgcatagc tactattcgg cccatcatca gccatatcac ctttaccagt acacgatatt
      181 tgttgctgaa gttgccagca catttaatga gcagttgctg catcgctatc tgatggggca
      241 ggccaaatcg gatgaggagc gggcctatta catcaaccat gagatcgacg aaatccgcgg
      301 gaccatcttt cgtcaaacaa tgtttgccga gtttgaaaag atgattcatg agcacgttga
      361 aaaaggtgat gcgctgacgg tcgaaacttt ccggacgatg tatcgctcgc ttctcaatga
      421 ttattttggg ccagatgtga aagtgcctga ccttcttgaa ctggaatgct tccgcatccc
      481 gcatttttac aacgcatttt atgtctataa gtacgccaca ggcctgtccg ctgccattgc
      541 gttggccgat cgcgtccttc aaggtggaaa gaatgaattg gacgcctacc tcgcgtttct
      601 ttccgcgggt tgttctaagg atcctctcga tcttttgcgg gacgcagggg tggatatgga
      661 acgccccgac ccggtggcgg ccgccatgga gcggttcgat acgctggttg acgaacttga
      721 aaagattctg tgacaggctg ggatcggcat ttttgaagcg agaaacgagc gtcgacatga
      781 tagcgcaaga gtcgtctgtt tcgctttcaa cttgggatga gtttcgttct ttgatgccca
      841 tctgtggccg gtatgtgtat tttgatcacg cggctgtggc acccctgcca ggtccgactc
      901 gacaagccat cgaatcgtgg cttctggagg caacagaaga tggtgacacc cgttggccag
      961 aatgggccca gcgcctcgag gcctttcgcc gtgacatagc cgagttcatc ggtgcactgc
     1021 ccgaagagat tgccctggtc cgcaatacga gccatgggat atcaatcgtg gctgaaggtt
     1081 ttccctggca agcgggcgac aatgtagtca tcccggccca agagtttcct gccaaccaat
     1141 atccgtggct tgccctggaa agtttcgggg ttgaagtgcg acgtgtcccg cccaagcatg
     1201 gtgttatcga cttgaaggcc ctcgaggagg cctgtgatgc ccggacgcgc attttgtctg
     1261 tgagctgggt ggattatctt tcgggttttc gcattgcccc ggcggagcta gccgcattgg
     1321 cccaccgata tggggcttat tttttcctgg atgcgattca gggtttaggg gtgtccccga
     1381 tagatgtcca ccaggtggag atcgacttcc ttgcggcaga tgggcataag tggctgctcg
     1441 ggccggaggg ggcaggggtc ttttattgtc gcgctccgct gtgggaaatg cttcgtccta
     1501 caagcattgg atggaacagc gtcgaacatc cttttgagtt cgacagaatc gacccaaact
     1561 ggaaaaaatc tgctgcccga tttgagggtg gatcgcaaaa catcattgga tttttaggat
     1621 tgcacgcttc tctgtcgcta cttcgacgtt atggcccagc ggcaatcagc cgacgggtcc
     1681 tcgacctcac gaacacaatc tgcgaagtgt tgcgaagtct cggtgccatt cttctcagcc
     1741 ggcgtgatga aggcgttgcc tcgggaatcg tgacattctc aattcctgat ctcgatgtta
     1801 ttcgtctccg ggaggctctg ctgcgacggc gtattgttct gagtgtccgt cagaaagccc
     1861 tccgtgtaag tccacatgcc tacaacaatg agcatgacgt tgcagcattt ggcgaggcac
     1921 tccgcgaggc ggtggatgaa tgccgggcca gttgatcgag aaagattttt aaggacgtga
     1981 atcttttatg tcgaacgaag ctcacactat cgcaaaaaga attattgaag cgcttgaccg
     2041 gcgcgatcac gaggagttgg ttcgccttct tcagaaggtt ccggagggtg aactaccgca
     2101 aaggctcgcc cagttgaccg aggctgatca aaggcggttg ctggaagcga ttccggccga
     2161 ggttgcgtcc agccttcttc aggaactgcc agagagtcaa gtaacacggc tgcttgagga
     2221 gctagcgcca tcgcaggcgg cggccatact cgatcatctt ccgagtgatg aacaggccga
     2281 cttgctgggg gcgctgccgg acgaagaggc cagcaaactg ttagaaatta tgaatccaga
     2341 agaagcctct gacgccaggc gacttcttca atttgccccg gataccgccg gcggtctcat
     2401 gatcacggag tacctggcat ataccgaaac ttgtaccgtg gaagatattg tcgaagacct
     2461 aaggatcaat gctgctcgtt atcgtagtta cgacgttcag tatttctata cggtcgatgc
     2521 tgagggtagg cttaccggcg tcctgcgggt gcgagatctt ttgctttctg aacctgatac
     2581 acagattgcg actatcgccg tccgccagcc ggtgagtgtc gccacggata cttctttgga
     2641 taagctcgag catctctttg cccattacca gttttacggg ctcccggttg tggaccgaga
     2701 cggccgactg gtgggattgg ttaggcgtta cgacgtggag gaagcgattg ccgccagggc
     2761 gaatcggaac tttctcaagg tggtggggat atctggtggg gaagaattgc ggtccatgcc
     2821 cctccgcgac cgtatcaagg gccgcacctc atggttgaca gttaacctca ttttggatct
     2881 tatcgcagca agcgtgatcc cgtttttcga agagacaatc agtgccgtta tcgccttagt
     2941 tttctttcta cccattatct cggatatggg cggaaacacg gggatccaga gcattggcgt
     3001 cacgctccgc gagctggcag caggtttgat cacccctcgg gatgcttggc gaacattctg
     3061 gcgagagctt ccggtcgctg tgattaacgg cttggtcgtc ggattcctcc tcgccggcgt
     3121 ggcgttggta tggcgaggga atccttactt gggaatcctc atcggctcgg ccctgacgtt
     3181 aaacacggtt attgccgcat gtctgggggc cgttttgcca cttggcttgc gccgtattgg
     3241 aatcgatccg gcggtcgcct caggccccat cctaaccacc attgcggacc tgacaggctt
     3301 tttcatggtc cttggtggcg cggccgttat cttaccgtac ctaacataaa accggtggcg
     3361 ggcaccaccg gttctgcggt ttacgacaga gccgaaaaaa gttcgcgtcg agcaatcacg
     3421 gaccgattgg acttaggtca cgttgcagga agtcccgcgg cccacggagg gtgtaatatg
     3481 ggtaagtaat ggcggccgtc ggtggaccag gctggatatt ctggccagga tc
//
LOCUS       DAOPTR010000330         3504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_21414_length_3504_cov_2.978255, whole genome shotgun sequence.
ACCESSION   DAOPTR010000330 DAOPTR010000000
VERSION     DAOPTR010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21414_length_3504_cov_2.978255"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(376..1332)
                     /locus_tag="PKI05_07370"
     CDS             complement(376..1332)
                     /locus_tag="PKI05_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52061.1"
                     /translation="MTQLERENYALESKIWELVSQLEQKQAELDACRSELERLKSRAG
                     ATSQKANPSSHPRELYAPTWPEGGDMLAPAPSVGSSTEPFALPEVSVTPPSRETSPER
                     ALPGSTSHRPPEPSPEEKPSPEENSVTRPLLLAPSNSDAEPTPDILPQETTRRASPLD
                     SSRVSSPASAIPISSPSSRGEKADQVGPMTERIILPPKFVTGRDYDGEDGDDGIAVLV
                     QPRDSRGEVILEPAAISVVVVDPAMTGPAARVARWDLQPEEVRSYAVRRGDVLGFLLE
                     LPWPNRLPEHEDLKVFVRYYAPDGRYLEAQSDVRVSLARSGS"
     gene            2076..2149
                     /locus_tag="PKI05_07375"
     tRNA            2076..2149
                     /locus_tag="PKI05_07375"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2110..2112,aa:Arg,seq:acg)
     gene            2729..>3504
                     /locus_tag="PKI05_07380"
     CDS             2729..>3504
                     /locus_tag="PKI05_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52062.1"
                     /translation="MVRCTALALSLPQQLSLRLLAIWSILLCASMGMVSPHILAGTFP
                     NLFTLNRVEADPNKDYWLSEENGPWMIMACSFTGDQAEQQARELVLELRKRYKLPAYM
                     YEKTFDLKAEGGSNPRRYRYQHGERIREVAVLVGDFPSVDDPRAQETLQKIKCYRPAA
                     LESRPEKSTSLTLAALRSIQQSLLTDDNEKKQKGPMRHAFLTTNPLLPDEYFAPKGVD
                     KFILALNEGYEYNLLNCPGRYSVQVAHFTGEVIIDQREIQA"
BASE COUNT          787 a          960 c          991 g          766 t
ORIGIN      
        1 gctcgaatcg ctgccggatt tctcgttcga ctccgacgat cgcttgccct caccctgacc
       61 ctctcccgga gggagagggg gaattttggg atagactcta ggcccaatag accaaatcag
      121 cccggtggcc agatgactgt cgccaaaatc gaaatctacg attattatgc ccaaagctgg
      181 gcggagttcg ttccggctgg tacacagcaa aattccctcg acggccaatc tttctcacaa
      241 tgtattgaaa gaattttgtc catcggggcc gtcaggcggc gggtgtcaag tcgatcgcca
      301 caaagtgctt ggtacccgat gatgcaagca tgtctcgtcg cgcgtcaaaa gagcgggcga
      361 ctatcgggca cggcgttacg aacccgatcg ggcgaggctg acgcggacgt cgctttgggc
      421 ctcgaggtag cgaccatcgg gggcgtagta tcgcacaaag accttcagat cctcatgctc
      481 gggaagacga ttaggccagg gtaattccaa taagaatccc aacacatcac cgcgacgaac
      541 ggcgtagctc ctgacctctt ccggttgcaa atcccatcgc gcgacacggg ctgctggccc
      601 tgtcatagcc gggtcaacaa caaccaccga gatcgcagcc ggttccagaa tgacttcgcc
      661 gcgggagtcg cggggctgga ccaacacggc aataccgtca tcaccgtctt cgccgtcata
      721 gtcgcgcccc gtgacgaact tcggcggaag tataatccgc tcggtcatgg ggccaacctg
      781 gtctgctttc tcgcctcggc tggagggact cgaaatagga atcgcggagg ccggggaaga
      841 cacacggctt gaatccagcg gcgacgctcg gcgggtggtt tcttggggga ggatgtcagg
      901 cgtcggctcc gcgtccgaat tcgacggggc cagcaataga ggacgggtca cgctgttttc
      961 ctcgggtgac ggtttttcct cgggtgatgg ctctggtgga cgatgtgaag tgctgcctgg
     1021 caaggcacgt tccggggatg tctcgcggct ggggggcgtc acactgacct cgggtaaagc
     1081 gaatggttca gttgaggacc caaccgatgg cgcgggggca agcatgtcgc cgccttcagg
     1141 ccaagtgggg gcatacagtt cccgtgggtg tgaagaagga ttagcctttt gggatgttgc
     1201 cccggcccgc gacttgagtc gctccaactc gctccggcat gcgtcgagtt cggcctgttt
     1261 ctgctccagt tggctgacca attcccagat cttgctctct aaggcgtaat tctcccgttc
     1321 aagctgagtc acaatttgag gtgtgcgtca ccctatggcg agggcaaccg ccgctaggca
     1381 caaaaggaca atgaatttcc ataatgaggc catgtgaagg tgtttagccg tcacggtgag
     1441 ggaccaagca tttcgatcta tcgcggaatg accctattac ctggaccgcg aaaataacgc
     1501 aagatcaatc tagacattgc cgtaagatag gcaaaatata ccccaccaat gtggcatagg
     1561 ttacggcata tctcgccggt gtcttttgac gaacggtgcg ctatttttcc cgcttgtcct
     1621 gaccatgctg ggcagtttta ataacctcaa cccgcgcgac gagaggcaac aagagttacc
     1681 cgacaccgct tcgcggcggg gataaatttc tcgggcacct gttgacacaa taccgcacca
     1741 aggggttaac gggttggcaa tagctccacc gccgcgaatg gaacacggag gggcgccgct
     1801 atcggcggta actcgagcgt tttctcgcgt gtaccgacag tgggggcacc gatggacatt
     1861 ttccgcctcg cagggggacc tgataaacgg gtgccctctt tccgggattg tcgaaaaccc
     1921 ttaaaatgct tatgaataac aaccgattcc gccgcgacgg gcgggaatga cataaagcgg
     1981 aaggtggatg gccaccattc gggttgtctg ccctcttgcc aaaaaaccgt tatgttcccg
     2041 ctgtactcgg ccgagtgcca gcggaaatag agaatgcgcc tctagctcaa ttggatagag
     2101 catcggtcta cggaaccgaa ggctgcaggt tcgagtcctg cgaggcgcgt ttttttgttt
     2161 cttggtccgg tcttgccggt cgaagcctcc caaaggttaa tctgtcgaac gagaaaggcg
     2221 caaattgtgg aggttgcgcg agatatttaa gttggggtag ttcttcccga gttgctttgc
     2281 tcggcttgtg acaattttgc tgttgatttt cacgtctgac ggtctgagcc tccgcaccgc
     2341 agaaaagaac gtctaaagga cagtctgcca ggccaattcg cgtgctaatt tgccgttcca
     2401 gaacagcgcc agacccattt attccactcg tgaatgggca tttatttgcc tgccaagccg
     2461 gcgcgaccgc ctgatctcat ccacataact acgacccacg cctgctgggc agcgaagggg
     2521 cgatccatcg tgggagtcgc agcgttggag gactaaatcg cagcggacat gatcgaggac
     2581 gcttgcgagg ccgaaaagtc ctcttttcct aaggcacaca atttgctaaa ctccgccgct
     2641 catgccgaag ttgtcggcta atagtgcaat ctgggcgtca ttcaatccgg caggacgctt
     2701 ggaaagagtt caccataagg agccaaggat ggttcgttgc acagcacttg cactttccct
     2761 cccgcagcag ctttctctga gacttctggc gatatggtcg atcctgttat gtgcgtcgat
     2821 gggcatggta tcaccgcaca tcctggccgg gacgtttccc aatctgttca ccttgaaccg
     2881 tgtcgaggct gatccgaaca aggattactg gctatcagaa gaaaatggcc catggatgat
     2941 catggcgtgt agtttcaccg gtgatcaggc ggaacaacaa gcccgagaat tggtcttgga
     3001 attgagaaaa cgctacaagc ttccagccta catgtacgaa aaaacgtttg acctcaaagc
     3061 tgaaggcgga tctaaccccc gtcgctatcg gtatcaacat ggcgagagaa tccgcgaggt
     3121 cgccgtcctc gttggagact ttccctcggt cgatgatccc cgtgcccagg aaaccctaca
     3181 aaagattaaa tgctatcggc cggcggcgct cgaatcgcgt cctgagaaat ccacctccct
     3241 aacactggcc gcgctgcgct caatccagca gtcgctgctt acggatgata acgaaaagaa
     3301 gcaaaagggc ccgatgcggc acgctttttt gaccacaaat ccccttttgc cggacgagta
     3361 ttttgccccg aaaggcgtgg acaagtttat cctggccctc aatgaaggtt acgaatacaa
     3421 cctgctgaac tgtcctggcc ggtattcagt gcaggttgcc cactttacgg gagaagtcat
     3481 aatcgatcag cgagagatcc aggc
//
LOCUS       DAOPTR010000331         3493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_21496_length_3493_cov_3.673647, whole genome shotgun sequence.
ACCESSION   DAOPTR010000331 DAOPTR010000000
VERSION     DAOPTR010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21496_length_3493_cov_3.673647"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PKI05_07385"
     CDS             complement(<1..463)
                     /locus_tag="PKI05_07385"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA52063.1"
                     /translation="MSKISRRTWLMGSALAASQGVFAGGVSGEPIPGFEQVQVETDPN
                     KPWEPYTDRKVRLGIVGHGYCKFGLAFDLQYHPNVELVAVSDLIPERCAEMAKLAKCE
                     KTYPSLEEMVKDDSIEAIFCATDAPSHARHAILCLEHGKHVATAVPAFYGDI"
     gene            complement(661..2028)
                     /locus_tag="PKI05_07390"
     CDS             complement(661..2028)
                     /locus_tag="PKI05_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA52064.1"
                     /translation="MRRLRFSRRRMLAAASALAVPYWFTHSRVMGQGGEEKNDRPRIA
                     AIGVGGRGTAILKSAARFGDVVAVCDADLQHAEAASQLFDGKPAVYQDYRKLLEERQD
                     IDVVLNGTPDHWHTAINVMACRLGKDVYTEKPLGLTIEEGKIFRQVVAETRRIVQVGT
                     QQRSVAHFRLACELVRNGRIGKLQRVIVTLPFWNTKGGPFKPQSVPPHLNWDLWQGQA
                     PERPYYPERVHFNFRWWSEYAGGIITDWGQHHMDIAYWGMDMENSGPLWVEGEAIFPN
                     EGKPDCYDNPDRFFVKMLFPGDIELLYFVAHDERYRATITSEEDVQLFNQAQLDLPET
                     SRNGIMFIGEKGRIFVNRGGAYGKAVEELKENPLPANAIRLYESDDHMANFFDCVKSR
                     KQPASTVAVAHRVLTACHVANIAIRLKRRIYWDATKEEIIGDAEAAQSIYVKRPQRPP
                     YTIEV"
     gene            complement(2405..>3493)
                     /locus_tag="PKI05_07395"
     CDS             complement(2405..>3493)
                     /locus_tag="PKI05_07395"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOA52065.1"
                     /translation="YNYNLAECLVPFSMISIWKHDIPYLKSKNCLGINLETLANWQIY
                     GPHIYLSIRLAYDPQADADAIMDDYFEKFYGPRAGPLMKQYWLDIDRAFVDLNCHAGG
                     FYAVHLVYTPDFLKHCRDLLERAAAAAEGEPACLARVRMAAEGLENAAQYETIRQAMN
                     RGDFVTALQTYENLLARSKKNVEEGWGNHYTVAYLERFLGPQVRAGAAVTASPNRLIS
                     VLPDKWRMTYDPDDQGVALGYNKKDYDDSGWHEVLTYSKPLDAQGLPDRQTILWYRTR
                     FSVPKTAKRLSLFFTEVDGDATVFVNGQEVGTSPKKRVPFEVDITSAITSGENVVAVR
                     VDHSQITELFLGGILRPVLLVERAAEKK"
BASE COUNT          748 a         1007 c          940 g          798 t
ORIGIN      
        1 cgatatcacc gtaaaaggcg ggcacggctg tagccacatg cttgccgtgt tccaggcaaa
       61 gaatggcatg ccgggcatgg gagggtgcgt cggttgcgca aaagatcgct tcaatggaat
      121 cgtccttgac catttcttcc aaagagggat aggtcttttc gcactttgcc agctttgcca
      181 tttcggcaca ccgttcgggg atgagatcac ttacggctac caattcgacg ttgggatgat
      241 actgcaaatc aaaggcgagg ccgaatttac aataaccgtg ccccacaatt cccaggcgaa
      301 ctttgcgatc tgtgtaaggt tcccatggtt tgttggggtc tgtttcgact tggacctgct
      361 caaagccagg gatgggctcg cctgaaaccc ctcccgcaaa cacgccctgt gaagccgcca
      421 gggctgaacc catcaaccag gttcgccgag aaattttaga catgatatat tctccttatt
      481 cctgggggcc cgtagattct ttatcttctg ccacgcctta ttatccgcgt gccaaactgt
      541 gcttgttccc aaaacccatc gccgtcaccg ctgcccggcc ggtgcagcat ttccccggag
      601 gcccacgtca ccggctcaca ttggtgatcg gcaattccga gtcgtttccg agtcgtgcgg
      661 ctagacttcg attgtataag gtggccgttg cggccttttt acatatatgg attgggcggc
      721 ttcggcgtcc cctatgatct cttccttggt ggcgtcccag tagatccgtc gcttgaggcg
      781 gatggcaata ttcgccacgt gacatgccgt caaaacccga tgggccacgg ccactgtgga
      841 ggccggttgc ttgcggcttt tcacacaatc aaaaaagttc gccatgtgat cgtcgctttc
      901 gtaaagacga atggcgttgg cgggaagtgg attttctttc agttcttcga ccgccttgcc
      961 gtaggcgcca ccacggttga caaaaatacg gcctttctcg ccgataaaca taatgccgtt
     1021 gcgcgaagtc tccggcaggt ccagttgggc ctgattgaag agctggacgt cttcctcgct
     1081 ggtgatggtc gcccgataac gctcgtcgtg cgccacgaag taaagcaact cgatatctcc
     1141 gggaaagagc atcttcacaa agaagcgatc gggattgtcg taacagtcgg gtttgccctc
     1201 gttggggaaa atggcttcgc cttccaccca gaggggaccg gagttttcca tgtccatgcc
     1261 ccaataggca atatccatat gatgctgccc ccagtcggta ataatgccgc cggcatactc
     1321 ggaccaccag cggaaattga aatgaactcg ctcgggatag taaggccgtt cgggggcttg
     1381 tccctgccat aaatcccagt tgaggtgagg tgggacggac tgcggcttga acggtccacc
     1441 cttggtgttc caaaagggga gtgtgacaat cactcgctgc aacttgccga tgcggccatt
     1501 tcgaaccaac tcacaggcca gccgaaagtg cgccacgctc cgctgttggg tacccacctg
     1561 gacgatccgc cgcgtttccg ccacaacctg gcggaaaatt ttcccttctt cgatggtcag
     1621 accgaggggt ttttcagtat agacatcctt gccaaggcgg cacgccatca cattgatggc
     1681 cgtgtgccag tggtctggcg tcccattaag gacgacatcg atgtcttgac gttcctcgag
     1741 gagcttgcgg tagtcctgat agacggccgg tttgccgtca aagagctggc ttgccgcctc
     1801 ggcgtgctgc aagtcggcat cgcacacagc cacgacatcc ccgaaacgtg ccgccgactt
     1861 gaggatggcc gtcccacgtc ccccaacgcc aatcgcggcg atgcgggggc ggtcgttttt
     1921 ctcctctcca ccctgaccca tgacacgtga atgggtgaac cagtaaggca ccgccaaggc
     1981 ggaggcggcg gctaacatcc gacgacggct aaaacgaaga cgccgcatat catggttcct
     2041 ccttcatcaa cggaacgcgg tttgcgcgcc cctatcgctg cttatgtcgc caaggccatg
     2101 gaccaaaaga cttcttttgc ccaccaggcg tgctttagca tagtgcgctt tattaccggt
     2161 tgtaaagcga ggcaaagcac gtggcacgat tcgctcctgg gtgtaggagc ctaatctccc
     2221 ctcacccggt cggcgggaga cgggtcgggc gtgaaagtag cttgaatcgc ttccggtttt
     2281 ctcaatcaat tccgacgttc gcttgccctc accccaaccc tctcccggag ggagaggggg
     2341 acttttgggg ataggctcta cgtagcatca agcatcaact gctgggcgat atcgcaagca
     2401 aggtctattt tttctccgcc gctctttcca ccagtagcac gggtcgcaga atgccaccca
     2461 aaaaaagttc agtaatttgc gagtgatcca cgcgcaccgc caccacgttc tcgcccgatg
     2521 tgattgcaga agtgatatcg acctcaaagg ggactcgctt tttcgggctt gttccaacct
     2581 cttggccatt aacgaagacc gtagcgtcac catcgacctc agtgaaaaag aggctcaaac
     2641 gcttggctgt cttcggcacg gaaaagcggg tgcgatacca caaaatagtc tgcctgtcgg
     2701 gcaaaccctg agcatccagc ggcttgctgt aggtgaggac ctcatgccat cccgaatcgt
     2761 cgtagtcttt cttgttgtag cccagcgcga cgccttgatc gtcgggatca taggtcatcc
     2821 gccacttgtc cggcaacacg gagatcagtc ggttgggaga agccgtgacc gctgccccgg
     2881 ccctgacctg cggcccgaga aaccgctcca agtacgcaac cgtgtaatgg ttgccccaac
     2941 cttcttcaac gttcttctta cttcgggcga ggaggttttc gtacgtctgc aaggccgtca
     3001 caaaatcacc tcggttcatg gcctggcgaa ttgtctcgta ttgtgccgca ttttccagac
     3061 cttcagccgc catgcgaact cgtgccaagc aagcgggctc cccttctgct gcggcagccg
     3121 cccgttccag caagtcccga cagtgcttga ggaagtctgg ggtatagacc aggtggaccg
     3181 cataaaaccc cccggcgtgg caattcagat ccacaaacgc acgatcaatg tccaaccaat
     3241 actgtttcat caaggggccc gcccgaggtc catagaactt ctcgaagtaa tcgtccatga
     3301 tggcgtcggc atcggcttga ggatcatagg ccagccgaat gctgagatag atgtgcggac
     3361 cgtaaatctg ccagttggcc agtgtctcaa gattgatgcc caagcaattt ttgcttttca
     3421 gatacgggat gtcatgtttc cagatggaaa tcatcgaaaa cggcacaaga cattcggcca
     3481 agttgtagtt gta
//
LOCUS       DAOPTR010000332         3487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_21535_length_3487_cov_3.900058, whole genome shotgun sequence.
ACCESSION   DAOPTR010000332 DAOPTR010000000
VERSION     DAOPTR010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21535_length_3487_cov_3.900058"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2681)
                     /locus_tag="PKI05_07400"
     CDS             complement(<1..2681)
                     /locus_tag="PKI05_07400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOA52066.1"
                     /translation="MDAYVSDDMYQEMLADFLDESTQLLDRLGENLLTLDEWVKNHAP
                     ESREQFDAEILNEMFRAAHSLKGLSAMLNLSNINQLTHKLENLFDAVRKGQMVVDDEA
                     VELMFQATDRLRAMVDLLRNQNSDAIDAQDVVTAIAAMLEGRQPGPSVAETRAMEALS
                     QAETALAEAMSDSAPDSSAVSQASPLPTDNARFQEEAAARFQDVHDEADLSSGYIALF
                     IDELAQGLDVLTETLLEAEGQGTSTQLENLLCTAHRIKGSAASVGFHRASKLAHLMED
                     ILQGLRETGRPLTRELADILLKCTDGFRSFLEQLRQGQSQYDADFVSLAADLLACASS
                     LKNPPAAAVSLTVPTPSPRETNQPLGVWPSAELLQTARNLTSGPSLVGRVEFQTNLDL
                     SGLKARLIYEKLCHQGNVVWFSVPSEQLDQIEKLDYVEFIIETSQPEESITRAIRVGG
                     VKQIHIQPLSKDSGAVNYKAGSSAKPSEMVPKQPVAEGKEKPTVSDGGGDQEKSRPSG
                     EAVKAVETLRVDIERLDQLMNLAGQLVITRARLTELNDKLKGFFQSNRASQVLRGLRD
                     LVGRIAGMSSLGSSSGGTSRLESTLADRLEKEFEALAHELEALDQARSTVNYLAEAVH
                     QLDRVTDGIQRAVMETRMVPIGPLFNRFKRVVRDVTRLNGKNIQLVINGEKTELDKRM
                     IDELGDPLIHLVRNAADHGIESPEVRRQLGKPEQGTITLNAFHRGNSIIIQVSDDGKG
                     LDTERILKKALERNLITPGQAERMSRQEIFQLIFLPGLSTAEKVTEVSGRGMGMDIVK
                     AKIEELSGTIDLDSVPGSGTTVTIKLPLTLAILPSLLVQVDGDVFAMPIEAVNEIVAV
                     PSEQLLTVHGLRTARIRERVISVVELSEIFGGTLSRS"
     gene            3067..>3487
                     /locus_tag="PKI05_07405"
     CDS             3067..>3487
                     /locus_tag="PKI05_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOA52067.1"
                     /translation="MSDSERTQEVRGPIEPTETGQGFRIPVADRVNRLPPYLFARINR
                     LLYQKRRAGADVIDLGMGNPSDSPEDIVIEKLASAARDPDNHGYTESRGILNLRREVA
                     SKYFKQYGVRLDPESEIIVCLGSKDGFSHLCLALLGPG"
BASE COUNT          752 a          952 c          980 g          803 t
ORIGIN      
        1 tgcgaccggc tcaaagtccc accaaagatt tccgagagct ccacaacaga gatgacccgt
       61 tcgcggatgc gggcggtcct cagtccatgc acggtgagga gctgctcaga cggcacggcc
      121 acaatctcgt tgacggcctc gatgggcatg gcgaagacat cgccatcgac ctggaccagc
      181 aagcttggca agatagcaag cgtcaacggc aacttgatgg taacagtcgt acccgagcca
      241 ggcacactgt cgaggtcgat cgtgccgctc aattcttcga tcttggcctt aacaatatcc
      301 atgcccatgc cgcggccgga gacttcggtt actttctccg ccgtactcag gccgggaagg
      361 aaaatcagct ggaagatttc ctggcgcgac atcctctcgg cctgaccggg cgtgatcaag
      421 ttccgctcca gggccttctt gaggattcgc tcggtatcca gtccctttcc gtcatcactc
      481 acctgaatga tgatcgaatt cccgcggtgg aaggcgttga gtgtgattgt gccctgctcg
      541 ggcttgccca attgacggcg gacctcgggc gactcgatgc cgtgatcggc cgcattgcgg
      601 acaaggtgaa tcaatgggtc gcccaactcg tcgatcatcc gcttgtcgag ttccgtcttt
      661 tcgccattga tcaccaactg aatattcttg ccgttgagtc gggttacatc ccgaacgaca
      721 cgcttgaaac gattgaacag gggaccgatg gggaccatcc gggtttccat gaccgcccgc
      781 tgaatcccat ccgtgacgcg atccaattga tgaacggctt cagcgagata gttaacggta
      841 cttctcgcct ggtcaagggc ctcgagttcg tgggccaagg cttcgaattc cttttcaagg
      901 cgatcagcaa gcgtactctc gaggcggctc gtcccacccg aggaagaccc cagagatgac
      961 atgcccgcga ttctcccaac aaggtcgcgg agaccccgca gcacctgcga cgccctgttg
     1021 ctttggaaga atcccttgag cttgtcgttc aactccgtca gtcgtgccct tgtaatcacc
     1081 aactggcccg caagattcat cagttgatcg agacgctcga tatcgacacg cagcgtttcg
     1141 acggccttga cggcctcacc tgacggacgg ctcttctctt gatcaccgcc gccatcgcta
     1201 accgtcggct tttccttgcc ttcggccacc ggctgttttg gaaccatttc ggacggtttg
     1261 gccgaactcc ccgctttgta gttaaccgcg ccgctatcct tcgataacgg ctggatatga
     1321 atctgtttga cgccgccgac tcgaatagcg cgggtgattg attcctcagg ctggctggtt
     1381 tctatgataa attccacgta gtcgagtttc tcaatctggt cgagctgttc cgagggtacc
     1441 gaaaaccaca cgacgtttcc ttgatggcag agcttttcgt aaatcagccg ggctttgagt
     1501 ccacttagat ccagattggt ctgaaactcg acacgtccga cgaggctagg accgctggtc
     1561 aaattgcgcg ccgtttggag caattcggcg ctcggccaga cgccaagtgg ctgatttgtt
     1621 tcgcgaggtg atggcgtcgg cactgtaagc gacaccgccg cggcaggggg attcttcagc
     1681 gagctagcgc aagccaggag atcggcggca agactaacaa aatccgcgtc gtattgggac
     1741 tggccctgtc gcagttgctc caagaatgag cggaagccgt cggtgcattt gagaaggatg
     1801 tcggctaatt cgcgggtcaa ggggcgtccc gtttcgcgca gtccctgaag aatatcttcc
     1861 atgagatggg caagtttgga cgcacgatga aagcctacgg atgccgccga ccccttaatt
     1921 cggtgagccg tacacaagag attctcgagc tgtgttgacg tcccctggcc ttccgcctcc
     1981 agaagagtct cagtaaggac gtccagacct tgagccaatt cgtcaataaa taaggcgata
     2041 tacccagagg aaaggtcagc ttcgtcgtgg acgtcttgaa atcgtgcggc ggcctcttcc
     2101 tgaaaacggg cgttatcagt gggcaacggg cttgcctggc tcaccgcaga agaatctggg
     2161 gccgagtcgc tcatggcctc ggccaaagcg gtttccgcct gagaaagcgc ctccatagcc
     2221 cgggtttcag caacggaagg gcccggctgg cgaccttcca gcatggctgc aatggccgtg
     2281 acaacgtcct gggcgtcaat cgcatcggag ttctgattgc gaagcagatc caccatggcc
     2341 cgcaagcggt cggtggcctg gaacatcaac tccacggcct cgtcatcaac gaccatttgt
     2401 cccttgcgaa ccgcgtcaaa gaggttttcc aacttgtggg tcaactgatt gatattggaa
     2461 agattgagca tggcggacag tcccttaaga ctgtgcgccg cgcggaacat ttcgttcaga
     2521 atttcagcgt caaactgttc gcggctctca ggggcgtggt tcttgaccca ctcgtccaag
     2581 gtcagaagat tctcgccgag gcgatccaaa agctgagtcg actcgtcgag gaaatccgcc
     2641 agcatttcct gatacatgtc gtcactgacg taggcgtcca taggctggtc tcacctaatc
     2701 cattggggaa agttactccg ttgggaagcg tcttcacagc tggtgagaat cactcggtgg
     2761 gaatttggat aaaggcgtgg ggctccgctt atttccgtcg ccactcaaca atagctcata
     2821 tttgggggga tgtcagaatt acctgcggaa agcacaagga tgcgaagact gtcccgatgg
     2881 agacaaggaa cggatttctg ggtaattccg agcgcttttt cgaaaaacaa ggaaattggg
     2941 agacttgggc ttttcctaag cgggcttcgg tcggtaaact tagcaaaaga tcgtggttgc
     3001 gattgcgcca cgctcatatt caatattttt gttgtatatt tctttgttgc catagaagaa
     3061 tttgacatga gtgactctga gcgtactcag gaagttcggg ggcccatcga gccaacagaa
     3121 actggccagg gatttcgtat tccggttgcc gatcgggtta atcggttgcc gccctatttg
     3181 tttgcgcgaa tcaatcgcct gctgtatcaa aaacgacgcg ctggggccga tgtgattgac
     3241 cttgggatgg gaaacccgag tgattcgccg gaggatattg ttatcgaaaa gttggccagt
     3301 gccgcccgtg acccggacaa tcacggttac acggagtccc gcgggattct taatcttcgg
     3361 cgggaggtgg cgtcaaagta cttcaagcaa tatggggtgc ggttagaccc ggaatcagag
     3421 attatcgtct gcctcggctc caaggacgga tttagccatt tatgcttggc attattaggt
     3481 ccaggcg
//
LOCUS       DAOPTR010000333         3487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_21537_length_3487_cov_3.084499, whole genome shotgun sequence.
ACCESSION   DAOPTR010000333 DAOPTR010000000
VERSION     DAOPTR010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21537_length_3487_cov_3.084499"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="PKI05_07410"
     CDS             <1..926
                     /locus_tag="PKI05_07410"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4432 family protein"
                     /protein_id="HOA52068.1"
                     /translation="LGWQAPVHGPVHPKWVNLWEPSGLGWLDGFDELLVRCGLESNGA
                     PEFDDKGILRYPLHGKIANRPACRVEVSMDSETGNITVRGEVYETRLFFTKLKLATEI
                     RTTVGACSLTVTDTVTNVAAVPGEMELLYHINFGPPVLEPGSKVTLPIQKLAPRDPIA
                     AAEIDRWDQYGPESPGKPETAYFVQPLGDGRGQTLALLHNEAGTNGVRVDFNIKELPC
                     FTLWKNEQPVCDGYVTGLEPGVNFPNRRSFEKAKGRVVTLQPGEARRFTVEITALVTS
                     DAVSTAREEILRLQTQAPPEVLRQPCLDWSP"
     gene            complement(1019..1513)
                     /gene="smpB"
                     /locus_tag="PKI05_07415"
     CDS             complement(1019..1513)
                     /gene="smpB"
                     /locus_tag="PKI05_07415"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOA52069.1"
                     /translation="MAKSKKPVDAEHDNERVVTHNRRAKHQYDVLETLECGIALRGSE
                     VKSLRNGKVSIEEAFARVKDGEVWLYNCDIAEFPQANRFNHEPKRPRKLLLHRREIKR
                     FASQASQKGLTLIPLKIYFKRGLAKVLLGLCRGRKEHDKREIIKKEESQREIRRMMQR
                     YQNR"
     gene            complement(1592..2482)
                     /gene="rfbA"
                     /locus_tag="PKI05_07420"
     CDS             complement(1592..2482)
                     /gene="rfbA"
                     /locus_tag="PKI05_07420"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOA52070.1"
                     /translation="MTLYCKKGIVLAGGAGTRLHPITRAISKQLLPIYDKPMVYYPLS
                     TLMLAGIRDILLISTPEDTPLFQRLLGDGSELGISIRYAVQPRPEGLAQAFIIGREFI
                     GSDHVAMVLGDNVFYGQGFQAMLIRAASRPVGATVFAYLVKDPQRYGVVELDKDGRPV
                     RIVEKPKEPRSNYAVTGLYFYDNDVVAIAASLKPSPRGELEITDVNQAYLERGRLHVE
                     ILGRGFAWLDTGTHESLLEASNFVETIEHRQGFKIACLEEVAYRKGFIDAAQLEKLAH
                     RYNNSYGQYLLDLLRQPGMR"
     gene            complement(2569..>3487)
                     /gene="rfbB"
                     /locus_tag="PKI05_07425"
     CDS             complement(2569..>3487)
                     /gene="rfbB"
                     /locus_tag="PKI05_07425"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOA52071.1"
                     /translation="PRHIFIQGDICDGVTVRRVLEEYRPWAIINFAAESHVDRSIDGP
                     AEFVQTNVVGTFRLLEAAREYWSQLPPSEKERFRFLHVSTDEVYGSLGPTGYFTEETP
                     YAPNSPYSASKAAADHFARAYHHTYGLPVLVTNCSNNYGPYQFPEKLIPLMILNALEG
                     KPLPVYGDGLNVRDWLYVEDHCRALRMVLHRARPGSEYNIGGNCEKTNLEIVHAICSL
                     VDRLRPDLPHRPCRQLITFVKDRPGHDRRYAIDASKIRRELGWEPQETFETGLERTVR
                     WYLENTTWVERVTSGKYRRERLGLGQTIS"
BASE COUNT          789 a         1024 c          918 g          756 t
ORIGIN      
        1 aactcggctg gcaggcgccg gtccatgggc cagttcatcc taaatgggtc aatctttggg
       61 aacccagcgg gttgggctgg ctggacggct tcgatgaact cctagtccgg tgcggtctgg
      121 aaagtaacgg cgcacccgaa tttgacgaca agggcatcct gcgctatccg cttcacggca
      181 agatcgccaa tcgcccagcc tgccgcgtcg aagtctctat ggatagcgag acgggcaata
      241 tcaccgtccg cggcgaggtc tatgaaaccc ggttattctt cacaaaattg aagttggcta
      301 ccgagattcg cacaactgtt ggagcatgct ctttaacggt gaccgacact gtgacgaacg
      361 tcgccgctgt gcccggcgag atggaactcc tttatcacat caattttggg ccaccggtct
      421 tggagccggg cagcaaagtc accttgccca tccaaaagct ggcgccacgt gaccccatcg
      481 ccgcggccga gattgaccgc tgggatcagt atgggccaga gtcgccagga aaaccggaaa
      541 cggcctactt cgtccagccg cttggggatg gccgagggca gacgctggcc ctgctccaca
      601 acgaagccgg gaccaatggc gtccgcgtcg atttcaatat taaggagcta ccttgcttca
      661 cactttggaa gaatgagcaa cccgtgtgcg atggttacgt cacaggccta gaacccggcg
      721 tcaactttcc caatcgcagg tcatttgaga aggccaaagg ccgcgttgtt acactccagc
      781 caggagaggc acggcgtttc acggtggaga tcacagcgct tgtcacgtcc gatgcggtat
      841 ccaccgcccg ggaggaaatc ctccgtctgc aaacacaggc cccgcccgaa gtgcttcggc
      901 aaccgtgcct agattggtcg ccgtgaaaac aaccagggac atgccaccac ggtgtacttt
      961 tttctcggtc cacgcgccgt gtatggattg gccctgaggc tgcgttagcc aggacggatc
     1021 agcgattttg gtagcgctgc atcatccgcc ggatttcccg ttgagattcc tcttttttga
     1081 tgatctcgcg tttgtcgtgc tccttccgac cacggcagag accgaggagc accttggcca
     1141 atccccgttt gaaatagatt tttagtggga tgagcgtgag ccccttttgt gaggcctgcg
     1201 acgcgaatcg cttaatttcg cgtcgatgaa gaagtaactt gcgcggccgt tttggctcat
     1261 gattgaaccg attggcctgc ggaaactccg cgatgtcgca attgtatagc cagacctccc
     1321 cgtccttcac gcgggcaaag gcctcttcaa tcgagacctt tccgttacga aggctcttca
     1381 cttcgctccc acgcaaggca ataccgcact ccagcgtttc caagacatca tactgatgct
     1441 tggcccgccg attatgggtc accacacgct cattgtcatg ctcggcatca accggcttct
     1501 tgcttttcgc cataacgttt cgcggatact tccttgaagg caacctatct cgccaaggtt
     1561 accattgtct cgcacatgtt gcgaccaacc tttacctcat ccccggctgc cgcaaaagat
     1621 ccaacagata ctgcccgtaa ctgttattgt aacgatgtgc cagcttttcc aactgggcag
     1681 cgtcgatgaa tcctttgcgg tacgccacct cctctagaca ggcaattttg aacccctgcc
     1741 gatgttcgat ggtttccaca aaattggacg cctccaagag cgattcatgc gttcctgtat
     1801 ccaaccaggc aaatccccgc ccgagaatct cgacgtgaag ccgccctctc tctaagtacg
     1861 cctgattgac atccgtgatc tcaagctcac cccggggact gggtttgagg ctagcggcaa
     1921 tcgccacgac atcattatca tagaaataca ggccggtcac ggcgtagttc gagcggggct
     1981 ccttcggctt ttccacaatc cgcacagggc ggccatcctt gtccaattcc acaacgccgt
     2041 accgctgtgg gtccttcaca agataggcaa acacggtcgc gcctacaggt cgagaagcag
     2101 cgcggatcaa catggcctgg aatccctggc cataaaagac attatcgccc aacaccatgg
     2161 ctacgtgatc tgagccgatg aattcccgac cgatgataaa cgcctgggcg aggccctccg
     2221 gtcgcggttg aacggcgtaa cggatggaga ttcccagttc ggaaccatcc cccaagagcc
     2281 gctgaaataa gggggtgtcc tcgggcgtgg aaatcagcag gatatcgcga attcccgcca
     2341 gcatgagggt ggagagagga taatacacca tcggcttgtc gtaaatcggc aggagctgct
     2401 tgctaatcgc ccgagtgatg ggatgcaacc gcgtccccgc ccctcccgcc aagacgattc
     2461 cttttttgca atagagtgtc acgctttaga cctcccagaa cattggtcgt gtcatgcgat
     2521 cggtcgctac tgttacttgc gaacttaccg aaaacgcgat cgctgccgtt aagagatcgt
     2581 ttgccctaat cccaatcgct cgcggcgata tttccccgac gttacccgct ctacccaagt
     2641 tgtgttttcc agataccatc tgacggtccg ttccaaaccc gtctcgaatg tctcttgcgg
     2701 ctcccatccc aattcacgtc ggatcttact ggcatcaatg gcgtagcgtc gatcgtgacc
     2761 gggccgatcc ttgacgaacg tgatcaattg acggcacggg cgatggggga gatccggccg
     2821 caaacgatcc acaagcgaac aaatggcatg gacaatctca aggttagtct tttcgcaatt
     2881 tccgccgatg ttgtattcac ttcccggtcg ggcacggtga agaaccatcc gcaaggcccg
     2941 gcagtgatct tccacataaa gccagtcgcg aacgttaaga ccgtctccat aaaccggcaa
     3001 aggcttgccc tccaaggcgt taaggatcat gaggggaata agcttttcgg ggaactggta
     3061 tggcccatag ttgttggagc aattcgtaac aaggaccggc aagccataag tgtgatgata
     3121 cgcccgagcg aaatgatcag ccgcggcctt ggatgcggaa tagggtgaat tgggggcgta
     3181 gggcgtctct tcggtgaagt aacccgtcgg accaagcgat ccataaactt catcggtgga
     3241 aacgtgaaga aagcgaaaac gctctttctc cgagggaggc aattggctcc agtactccct
     3301 ggccgcttcc agaagccgaa acgtgcccac tacattcgtc tgaacgaact ccgccggccc
     3361 gtcaatggaa cgatccacat gcgactcggc cgcaaaattg ataatagccc agggacgata
     3421 ctcctctaaa acccggcgga ccgtcacacc gtcacagata tcgccttgaa tgaagatatg
     3481 ccgggga
//
LOCUS       DAOPTR010000334         3477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_21621_length_3477_cov_2.387200, whole genome shotgun sequence.
ACCESSION   DAOPTR010000334 DAOPTR010000000
VERSION     DAOPTR010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21621_length_3477_cov_2.387200"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PKI05_07430"
     CDS             complement(<1..255)
                     /locus_tag="PKI05_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52072.1"
                     /translation="MDRDLLRRGKSLSRQRHAGRMFVATLSLAVFFTIVSSQVALSNS
                     ADVLRFDLPELPSPEIASIANRVYLVLEKQAHFLLDQLKPW"
     gene            complement(367..1359)
                     /locus_tag="PKI05_07435"
     CDS             complement(367..1359)
                     /locus_tag="PKI05_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HOA52073.1"
                     /translation="MKAAYIDHPGPPEVIRYDDLPVPQLKETEVLVRVGAVSVNPIDT
                     YIRAGTVKMPLPKPYILGCDVAGTVEAVGARVTRFKPGDRVWGSNQGLLGRQGTFAEY
                     AAVDECWLYPTPATVSDREAAAIALVGITAYLGLFRDANLRPNETVFVHGGTGGVGSC
                     VVQMAKAVGARVITTAGNEEKADLCRQLGADVVINYKQENEAEAIRRFAPEGVNVWFE
                     TLREQNLEMAVSLLAMRGRLVLIAGRQSKPKFPVGSFYVKDCKAFGFAMFNAPPEEQQ
                     RAAEQINQWLKNGKLRPVIGRVLRLSQAVEAHRLQEQNTLGGAGNLVGKIVLEP"
     gene            complement(1409..1906)
                     /locus_tag="PKI05_07440"
     CDS             complement(1409..1906)
                     /locus_tag="PKI05_07440"
                     /inference="COORDINATES: protein motif:HMM:NF025559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HOA52074.1"
                     /translation="MNQPDKLEQLRQQLLDSYKNRALIYYHIYDELARILGPDQAEEI
                     LKRAIYRRGQERGREYARFAPNNLAAVKETFLAHLPDDGRLFEPEILTDTPDALDIKF
                     HRCPLREAWVEAGLPDELVARLCRIAAEVDYGLFREAGFGFFADTWQPGGEGCCCLHI
                     RPGGA"
     gene            2114..2884
                     /locus_tag="PKI05_07445"
     CDS             2114..2884
                     /locus_tag="PKI05_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52075.1"
                     /translation="MASIDSALSAELLPMPSPEIVAQLRRNISTIHRSYRPRQGQKFP
                     IDIPEVMDLFPGGQLPYGSIMEWFTETHGTEAESLSLRIGWSACLRNRFLVIVDPCFE
                     IYPPGLSQLGIDLNRVVFVHPATKSDMLWTIDQALRCSAVGAVWSYLESLDPINHRRY
                     QLASECGGTVGIFVRPLRARKESSWAEFRLLVQPVAFPAGEIGSQCRRIQVTLLRAPG
                     LSEPIVREVWIDDPADRLLDIQRVEPFQRRYQYQKNRA"
     gene            2940..>3477
                     /locus_tag="PKI05_07450"
     CDS             2940..>3477
                     /locus_tag="PKI05_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52076.1"
                     /translation="MAEICLRYTPHVGLVEDPHSDSLFLELTDIQHLIPDERRFVIQL
                     LMDCHREGYFPTKIAVADTPGAAWAMTHFGTGPRATEISSAWILPPSESKKWIASLPV
                     EALRIPDLVAEWCHELGLHRIGDLIHFPMAELRKRFGEILVLRLEQLLGSRQEILSPY
                     LPQPQFEEHLWLEYPARSW"
BASE COUNT          778 a          947 c          915 g          837 t
ORIGIN      
        1 ccaaggtttt agctgatcca gcaaaaaatg agcctgcttt tcgaggacca gataaacccg
       61 attagcaatc gaggcaattt ctggtgatgg taactccggc aagtcaaagc gcaagacgtc
      121 ggcactgttt gagagagcaa cctgtgatga gacgatggtg aaaaagacgg ccagcgataa
      181 cgttgccacg aacatacgtc cggcatgccg ttgacgcgat aaagatttgc cccgccgaag
      241 taggtctcgg tccatgctgc aaatcgtaat tcaaaaaggg acacgtaggg ataaaccact
      301 cggtaacacc accgatcatt ccgacagtcc cattgtgccc caagcgaact aaggggacaa
      361 tcccttttac ggttccagga caatcttccc gacgaggttc ccggcaccgc cgagtgtgtt
      421 ttgttcttgc aaccgatggg cctccactgc ctgtgagagt cgaagaaccc tgccgatcac
      481 agggcgaagt tttccatttt tcaaccattg attgatctgc tcggcggcgc gctgctgttc
      541 ctctggcggt gcattgaaca tggcgaagcc gaaggccttg cagtctttca catagaaaga
      601 tcccaccgga aattttggct tggattgacg cccggctatt aagacgagac gtccgcgcat
      661 cgctagtagc gataccgcca tttccagatt ctgctcgcgg agggtttcaa accaaacatt
      721 gaccccctct ggggcaaagc gacggatagc ttcggcctcg ttttcctgct tgtaattgat
      781 caccacatcc gcccctaatt ggcggcacaa atcggctttt tcctcgtttc cggcggtggt
      841 aatcaccctg gccccgacgg ccttggccat ttgaaccaca caggaaccga cgccgcccgt
      901 cccgccgtgc acaaatacgg tctcattggg tctaaggtta gcatcgcgga atagtcccaa
      961 ataggccgtg atgcccacaa gggcaatggc tgcagcttct cgatccgaga ccgttgctgg
     1021 ggttggatac agccagcatt catccaccgc cgcatattcc gcgaacgtgc cctgccgccc
     1081 caaaagtcct tggtttgatc cccagacacg gtcacccggc ttgaaccggg taacacgggc
     1141 accgacggct tccactgtgc ctgccacatc acagccaaga atgtagggct ttggtagggg
     1201 cattttcact gttcccgccc ggatatatgt gtcaatgggg ttgacggaaa cggctccgac
     1261 tcgtaccaga acctctgttt ccttaagttg aggcacgggc aaatcgtcgt aacgaattac
     1321 ttcaggaggt ccaggatggt cgatataggc tgctttcatc gtcgacatcc tttcatcgag
     1381 tgaatgcaaa ggacttagct ttccacagtc atgcaccgcc gggacgaatg tgcaggcaac
     1441 agcatccctc gccgccgggt tgccaagtgt cggcgaaaaa gccgaatcca gcttcgcgga
     1501 atagtccgta atcgacttcg gctgcaatcc gacagagtcg ggcaacaagc tcgtcaggta
     1561 ggccggcctc tacccaggcc tcgcggaggg gacaacggtg aaatttgata tccagtgcgt
     1621 cgggcgtgtc cgtcagtatc tccggttcaa aaagtcggcc atcgtccggg aggtgggcta
     1681 ggaatgtctc tttgacggcc gcgagattat tgggggcaaa tcgagcgtat tcccttcccc
     1741 gctcctgacc ccgacgataa atcgcccgct tgagaatctc ttcggcctgg tccggaccca
     1801 atatacgagc cagttcgtcg tagatgtgat agtaaatgag tgcccgattc ttgtaggaat
     1861 caagtaactg ctgacggagt tgctccagtt tgtccggctg attcatggca gtgatcctga
     1921 tctgggaaag gccttttgag taattttgtt ctggtgaaat tataggctga cgcatcggca
     1981 gaaggccact ggaagtgcat cactccggac ggatggcagg ccgtctcctg tcctataagt
     2041 gttcctatga acacgtaaaa gcgtcttgac gatgttagga tacaaatgta cactacatgg
     2101 ggaggtaacg aagatggcca gcatcgatag cgctctctct gccgaacttt tacccatgcc
     2161 gtctccggaa atcgtggcac aattacggag gaacatctcc actatccacc gatcttaccg
     2221 accgcgccag gggcagaaat tccctatcga cattcctgag gtcatggacc tctttcctgg
     2281 gggacaatta ccctatggtt ccattatgga atggtttaca gaaacccatg gaacagaggc
     2341 cgaatccctt tcgttgcgaa tcggatggag tgcctgtctg cggaaccgat ttttggtgat
     2401 tgttgatcca tgtttcgaga tttatcctcc cggtctttcc cagttgggta tcgacctcaa
     2461 tcgagttgtc tttgttcacc ctgcaacaaa atcggacatg ttgtggacca ttgatcaagc
     2521 gcttcgatgt tcggcggtag gggctgtgtg gtcctatctc gaatcgcttg atccgatcaa
     2581 tcatcgtcgt tatcaacttg cttcagaatg tgggggaaca gtgggaattt ttgtgcgacc
     2641 tctcagggcg aggaaagagt cttcgtgggc agagtttcgc cttttggttc agccggtagc
     2701 ttttcccgcc ggagaaatcg gttcccaatg tcgacggatt caagtcacct tgttgcgagc
     2761 tcctggactt tcggagccca ttgtacggga ggtatggatc gatgacccag cggatcgctt
     2821 gcttgacatt cagcgggtgg aacctttcca acgccgctac cagtaccaaa aaaacagggc
     2881 ataatcacgc ttcttcaccc ggtcttcaag aagatgatgt tcggaagcgt caggaccgct
     2941 tggccgaaat atgcctccgc tacactcccc atgtgggttt ggttgaggat ccccattcag
     3001 attctctttt tcttgaatta acggatattc agcacctcat ccccgatgaa cggcggttcg
     3061 ttatccagct gctgatggac tgccatcggg aaggttattt ccccacgaaa atagccgttg
     3121 ccgatacccc tggcgctgcc tgggccatga cgcattttgg caccggccca agggcaacgg
     3181 agatttcctc ggcatggatt cttcccccgt cggagagcaa aaagtggatt gcatccctgc
     3241 ccgtcgaggc cctgcggatc cctgatttag tggccgagtg gtgtcacgag ttgggcctgc
     3301 atcgtattgg cgatcttatc catttcccga tggcagaatt gcgaaaacgc tttggggaga
     3361 tccttgtcct acgtctggaa caacttctgg gaagccggca agaaatcctc tcgccctatc
     3421 ttccccagcc acaatttgag gaacatctct ggttggaata tcccgcccga tcttggg
//
LOCUS       DAOPTR010000335         3473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_21644_length_3473_cov_3.371562, whole genome shotgun sequence.
ACCESSION   DAOPTR010000335 DAOPTR010000000
VERSION     DAOPTR010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21644_length_3473_cov_3.371562"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="PKI05_07455"
     CDS             <1..981
                     /locus_tag="PKI05_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOA52077.1"
                     /translation="NPWQTVSLWHACRVAKETLLGENPPEKYPVTILGRGSRLIGGTV
                     SVEVDRESIAQFLLNGFFPICDVTDRPERRRASGFREIGLPFESDTAVTRHLAAFLQS
                     HGSSKGQPARPTHVLLNGGVFKAKPFQERLLQVLTHWFSESPSPQLLSGVHDLDHGVA
                     KGAAYYGWAKDHGGVRIRGGTARAYYVGIETAGPAVPGIPRPLRLLCVVPRGMEEGTA
                     TDVPSDEIGLIVGEKVIFRFFSSSTRKDDRPGDVHESWSPDEIEETDSLEGCLPADEG
                     VQGDYVPVRLESRVNELGMLELWCLSTTDDKKWKLEFSVREDADAEVSPT"
     gene            1020..>3473
                     /locus_tag="PKI05_07460"
     CDS             1020..>3473
                     /locus_tag="PKI05_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOA52078.1"
                     /translation="MANIDEIEAKPPRFVVGMDLGTTNFAVCYVDTAESPWQIRTLSI
                     PQLVAPGVVESREILPSFHYEPAPGEFDHALLRLPFGPAQPQYIVGVLARDHGANVPG
                     RLISSAKSWLCHSGVDRTAPLLPWHGAPDVTRLSPVEVSARYLGHIVQVWNHQFPNYP
                     LAEQDFVLTIPASFDEIARQLTVKAAALAGLNRITLIEEPQAAFYSWIYRHAQSWPQV
                     IKPGQKILVCDIGGGTTDLTLIRVRRAEDGTFRFHRVAVGEHLILGGDNLDLALAHHI
                     ESKLGEDEKLDPQTWSVLVRKCQRLKEELLGPQAPERLSVSIPGRGTRLIGGARRVEL
                     ERAEVVRLLVDGFFPIVELNDKPNVRRIGFQEFGLPYAPDPAVTRYLAAFLTAHRYVA
                     LDETNPAYDPARPDLILFNGGLFFSEVIRQRVLDVVTYWFAKEDKSWQPIAIENPRLD
                     LAVAEGAAYYGMVRRGEGVRISAGLARSYYLGVDVPHPNVVAASVARQGKARFSWTTE
                     EEANVSGASPSAAQIEEASTRECLGFGMCVIPSGVEPGQEVSLKEKTFRLRVGEPVEF
                     PVYYSGTRLTDQPGDLIPVYREELTPLPPIRTVLKTGKRSSASELDVTIHAQLSEIGT
                     LELWCAQVEGRGTWRLEFDVRSAVQTDLRPHEGLGESEGVLDEETASRCLALIDHVFS
                     AEGRDPPENLLKRMSQVTEMNRKDWPPTFLRRIWQRLIENEVGRRRSAAHEARWLNLT
                     GFALRPGFGLAADDWRVSETWRILHGKLFHPTPACRAEWWVLWRRIAGGLTAGHQQAL
                     ANPLLASFRSFHKQQTTGTG"
BASE COUNT          691 a          976 c         1046 g          760 t
ORIGIN      
        1 aacccctggc aaacggtttc gttgtggcat gcctgccgtg tcgctaagga gactttactg
       61 ggcgagaatc ccccggagaa ataccctgtc acgatcctcg gccggggcag ccgattgatt
      121 ggcgggaccg tttccgtcga ggtggaccgg gagagtatcg cccaatttct actcaatggt
      181 ttcttcccca tctgtgatgt gacggatcgc ccggaacgga ggcgggcctc cggcttccgc
      241 gagatcggtc ttccctttga atcggatacc gctgtgacgc gccatttggc ggcgtttctc
      301 cagtcgcatg gctcgtccaa aggccagccg gcacgaccaa cgcacgtctt actgaatgga
      361 ggggtcttca aggccaaacc cttccaggaa cgtttgctac aagtcctcac tcactggttt
      421 tcggaaagcc catcgccaca attgcttagc ggagtgcatg atttggatca cggtgtggcc
      481 aaaggggccg cttattacgg ttgggccaag gatcatgggg gcgtccgcat tcgcggtgga
      541 acggcacgtg cctactacgt gggcatcgaa acggccggcc cggcagttcc cggtattccg
      601 cgccccttac gtttgctctg tgtggtcccg cggggcatgg aggaaggcac cgcgacggat
      661 gttccctcgg atgaaatcgg ccttattgtc ggtgaaaaag tcatctttcg attctttagt
      721 tcttccacac gaaaagatga tcggccggga gacgtccacg aatcgtggtc tccggacgag
      781 attgaagaga cggattcgct tgagggttgt ctgcccgccg atgaaggtgt ccagggagat
      841 tacgtccccg tgcggttgga aagccgagtt aacgagttgg gaatgcttga gttgtggtgc
      901 ttaagcacga cggacgacaa gaagtggaaa ctcgaattca gcgtgcggga agacgcagac
      961 gctgaagtct ccccaaccta atagtcttcc actctcgatg gggtcgatca gaagatgcca
     1021 tggcaaacat agatgagatc gaagcaaaac ccccgcgctt cgttgtgggg atggacctcg
     1081 gaacgaccaa tttcgccgtt tgctacgtcg ataccgcgga atctccttgg caaattcgga
     1141 ccttatccat cccacaatta gttgcaccgg gcgtggtgga atcgcgggaa atcctgccat
     1201 cgtttcacta tgaaccggca cctggcgaat tcgatcacgc tctgctccgt ctcccttttg
     1261 ggccggccca gccccaatat atcgtcggtg tcctggcacg ggaccacggc gccaacgtac
     1321 cggggcggct catttcctcc gctaaatcgt ggttgtgcca ttccggagtg gatcgcacgg
     1381 caccgcttct tccctggcac ggcgccccgg atgtcacacg tttgtcccct gtggaggtct
     1441 cggcacgcta cctcggacac attgtgcagg tctggaacca ccaatttccg aactatccgt
     1501 tggcggagca agattttgtc cttaccattc cagcctcatt tgacgagatc gcgcgacagc
     1561 taaccgtcaa ggcggcggca ctggctggtt tgaatcggat cacactcatc gaagaacctc
     1621 aggcagcctt ctattcgtgg atttaccgcc acgcccagag ttggccgcaa gtcattaagc
     1681 ccggccaaaa gattctggtg tgtgatatcg gcggtggaac aacggacctg acgctcattc
     1741 gggtacgtcg cgcggaggat ggcacatttc ggtttcatcg tgtggcggtg ggtgaacact
     1801 tgatccttgg tggtgacaac ctcgatctgg ctttggcgca ccacatcgag agcaaactcg
     1861 gggaagacga aaagctcgat ccccagacat ggtcggtcct ggtgcggaag tgtcagcggc
     1921 tcaaagagga gttactaggt ccgcaggctc cggagcgatt aagtgtcagt atccccggtc
     1981 ggggaacgcg tctgatcggt ggcgccaggc gtgtggaact ggaacgcgcg gaagttgtca
     2041 gattgttggt tgatggattt ttccctatcg tcgagttgaa tgacaagccc aacgttcgcc
     2101 gtattggttt tcaagagttt ggccttcctt atgcacccga tccagccgta acacgttatc
     2161 tagcggcatt tttgaccgct catcgctacg tcgctttgga tgagacaaac cccgcctacg
     2221 atcccgcccg cccagatttg atcttgttca atgggggcct gttcttctcc gaagtaattc
     2281 gccaacgggt cctggacgtg gtaacgtatt ggttcgcgaa ggaggacaag tcgtggcagc
     2341 cgattgcgat tgagaacccg cggcttgacc tcgcggtggc cgagggagcc gcctactatg
     2401 gcatggtccg tcgcggcgaa ggcgtgcgga tttcggctgg tttggcccgc agctattatc
     2461 tcggagtcga tgtcccccat ccgaacgtcg tggcggcatc ggtcgcccgg cagggaaagg
     2521 cacgtttctc gtggacgacg gaagaagagg ccaacgtgtc cggagcatcg ccatcggctg
     2581 cgcagattga ggaagcctcc actcgtgaat gtctcggctt tgggatgtgt gtcattccgt
     2641 cgggcgttga gccgggtcaa gaagtttctc tcaaagaaaa gacatttcgc ctccgtgttg
     2701 gcgagccggt ggagtttccc gtgtattatt cgggcacgcg cctcaccgat cagccgggcg
     2761 atcttattcc cgtgtaccgg gaagagctaa caccactgcc gcctatcagg accgtcctga
     2821 aaaccggaaa acggtcctct gccagtgagc tcgacgtaac aatccacgcc cagctttcag
     2881 aaatcggaac gctggaattg tggtgcgccc aagttgaagg acgaggaacc tggcggctgg
     2941 agttcgacgt ccgttcggcg gtacagacgg atctccgacc gcacgaagga ctgggagaaa
     3001 gcgagggtgt gctggatgag gagacggcat ctcgttgctt agctttgatt gaccacgtct
     3061 tttctgcgga aggacgcgat ccgccagaga acctgctcaa gcggatgagc caggtgacgg
     3121 aaatgaaccg caaggattgg ccgccaacct tccttcgtcg catttggcaa aggctcatcg
     3181 agaatgaggt gggacggcgg cgaagtgccg cccacgaggc acggtggctc aatctcacgg
     3241 gttttgctct tcggcccggg ttcggcctgg cggcggatga ctggcgggtg tccgagacct
     3301 ggcgcatcct tcacggcaag ttgttccacc ctacccccgc gtgccgggcc gagtggtggg
     3361 tcctttggcg ccgcatcgcc ggtgggttaa ccgcgggcca tcaacaggcc ctggcgaacc
     3421 cactcttggc ctctttccga tccttccaca aacagcagac caccgggacc gga
//
LOCUS       DAOPTR010000336         3471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_21658_length_3471_cov_3.724532, whole genome shotgun sequence.
ACCESSION   DAOPTR010000336 DAOPTR010000000
VERSION     DAOPTR010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21658_length_3471_cov_3.724532"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1466
                     /locus_tag="PKI05_07465"
     CDS             <1..1466
                     /locus_tag="PKI05_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOA52079.1"
                     /translation="GFNPIVWCRYVATAEYVAQYLPAVLPSGVQVTCVTGRLGDEERQ
                     VKIKEIDAQRPRVLVATDCLSEGINLQEKFNAVLHYDLPWNPNRLEQREGRVDRFGQP
                     APQVKAVRFFGRNNPIDGVVIDVLLDKARRIHDTLGTYVPVPEEAESVTQAILEALFF
                     RERRGSTRQGLLDLGVPEVEELHRRWERDAERERVNRTRFAQRALKPEEVQRELEATD
                     SVLGDPDAVREFILTAAQRLNLTITPDRRPDVFRVAVGRDVLTTLPEAISRALPESKT
                     GQWLISFTSPTPEGAEYVGRNHRFVSALARYLMELALSRGTARDTDRAMPAVVCRCGA
                     IRTGAVSRLTTILLLRVRYLVDQPNREPLLAEEILVTGFAHTLPSPSGRAAGGEGSKP
                     WQWLRDDDALRLLAAQPDANITLPEKQELVQTSLGLWNALQSDLQQRIRDRATELESS
                     YKRVRKAISLAVRNLRVTPQFPPDHLGLLVLQPVVKS"
     gene            1463..>3471
                     /locus_tag="PKI05_07470"
     CDS             1463..>3471
                     /locus_tag="PKI05_07470"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOA52080.1"
                     /translation="MTISSIRIEGGLLGPDILEQLLTGELPGQKPGDFGLPGKRNLTD
                     EIAAVFADARALWGVFQNRLQRLSETDMATSVTRDAWVIPFLGLLGYELRFNQKAYEV
                     DGLTFAISHRAYYPASSGKGARDERTPPSPSGRGAGGEGEGDDNAPPVHIVGARQELG
                     RLPPSGRPRLAPHSLVQEYLNRTEHLWGIVTNGLVLRLLRDSTYVRRQAYIEFDLRQI
                     FEGQLFNDFVVLYRLLHRTRLPRGMADAKDCLLEKYYQHGLEQGGRVREGLRDGVEEC
                     LKRLAEGFLSHPANEDLRRRASPDYTGNDRITPEEFYRQLLVLVYRFLFLLVSEDRGL
                     LSSDPLYREHYSVGRLRRLLDRQSASTEHDDLWCSLRVLWLVLVKDQPHQALGNKPLA
                     AALGLPILNGQLFDRLDLDACWITNRDLLEAFWYLAYYRPEPNAPPRRVNYAALDVEE
                     LGSVYESLLDYHPVITSDGRGRLRFDLHPGLERKSTGSYYTPAELVAELIRSALDPVI
                     AERLKAAKTPEEKDRALLSIRVCDPACGSGHFLLAAARRLGKELARIRTGEEEPAPER
                     VREAIRDVVSHCIYGVDKNPLAVDLCRVALWLESHTGDKPLTFLDHRIRCGDSLVGVF
                     DLSVLRRGIPDEAFAPVTGDDKGFARTVAKRNREECRGYRDLIHW"
BASE COUNT          633 a         1120 c         1022 g          696 t
ORIGIN      
        1 aaggttttaa cccgatcgtt tggtgccgct atgtcgccac cgccgagtat gtagcccaat
       61 atttgcccgc agtccttcct tctggcgtcc aagtcacatg cgtcacaggc cggctgggcg
      121 acgaggagcg gcaggtgaag atcaaggaaa tcgacgccca acggccgcgg gtgctcgtgg
      181 cgacggactg cctttcggag gggatcaacc tccaagagaa gttcaacgcc gtccttcatt
      241 acgatctgcc ttggaatccg aaccgcttgg agcagcgcga aggccgcgtg gatcgctttg
      301 ggcagccggc cccgcaggtc aaggcggtcc ggttcttcgg ccgtaacaac ccaatcgacg
      361 gcgtggttat tgatgtcctg ctcgacaaag cccgcagaat tcacgacact ctgggtacct
      421 atgttcctgt gccggaagag gcggaaagcg tcacccaggc cattctcgag gcactgttct
      481 tccgtgagcg tcgcggctct acgagacaag gactgctgga ccttggagtt cccgaagtag
      541 aggagctcca tcgtcgctgg gaacgtgacg ccgagcgaga gagggtcaac cgcacgcgct
      601 ttgctcagcg agcccttaaa ccagaggaag tccagcgcga gcttgaagcg accgatagcg
      661 tcctgggcga tccggatgcg gtgcgggagt ttatcctaac ggcagcccag cgactcaatt
      721 tgacgattac gcccgatagg cgtccggatg tatttcgcgt ggcggttggc cgggacgttc
      781 taaccacgct gccagaggcc ataagccgtg cccttcccga gtccaaaacc ggtcagtggc
      841 tcatcagttt tacctcgccc acgcccgagg gcgccgaata cgtcggccgc aatcaccgct
      901 tcgtctccgc cttggcacgg tatcttatgg agctggcact cagccgtgga acggcgcgag
      961 atacagatag ggcgatgccc gcggttgtct gccgttgcgg ggccatccgc actggcgccg
     1021 tctcgcgttt gaccacgatc ttgctcctgc gggttcgcta ccttgtcgat cagcccaacc
     1081 gggagccgct ccttgccgag gagatcttgg tcactgggtt cgcccacact ctcccctctc
     1141 cctctgggag agcggccggg ggtgagggca gcaagccctg gcagtggctc cgcgacgacg
     1201 atgccttgcg gctactggct gcccagcccg atgccaacat cacgttgccc gaaaagcaag
     1261 agctcgttca gacctcgctg ggcctctgga atgccttgca atccgacctc cagcagcgga
     1321 tcagggaccg cgcaaccgag ttggaaagca gctataagcg ggtgcggaag gccatctcgc
     1381 tggctgtccg taatttgagg gtaactccgc aatttcctcc agaccatctt ggattacttg
     1441 tattgcagcc ggtggtgaag tcatgacgat ttcctccata cgcatcgaag gtggcttact
     1501 tgggccggat atcctggaac aactgctgac cggagagctt cccggccaga agcctgggga
     1561 cttcggcttg cccggaaagc gaaacctaac cgacgaaatc gcggcggtct ttgccgatgc
     1621 ccgagccctc tggggcgtgt tccaaaatcg cctccagcgg ctttccgaaa cggacatggc
     1681 caccagtgtt acccgcgatg cctgggtcat cccgtttctt ggtcttctcg gctacgagct
     1741 tcgcttcaac cagaaggcct acgaagtgga cggattgacc ttcgccattt cgcaccgcgc
     1801 ctattacccc gcttcatccg gcaaaggagc cagggatgag cgtactcccc cctctccctc
     1861 tgggagaggg gcagggggtg agggcgaggg cgatgacaac gctccgccag tccacatcgt
     1921 cggcgcccgg caagagctcg gccgtcttcc tccctcgggc cgaccccgcc tggcgccgca
     1981 ttccctggtt caagaatatc tcaaccgcac cgagcacctc tgggggatcg tgaccaacgg
     2041 gctggtgctc cgcttgctcc gcgattccac ctacgtccgc cgccaggcgt atatcgagtt
     2101 cgatcttcga caaattttcg agggccagct attcaacgat tttgtcgtcc tctaccgcct
     2161 gcttcaccgc acccgcttgc cgcgcggcat ggccgacgcc aaggactgcc tgctggagaa
     2221 gtattaccaa catgggctgg agcagggcgg gcgggtccgc gaaggtctcc gcgatggcgt
     2281 cgaagaatgt ctcaagcgac tggccgaagg ctttctcagc catcccgcca acgaagacct
     2341 gcggcgtcgc gcctcgcccg attataccgg gaatgatcgc atcacccccg aagagttcta
     2401 tcgccagttg ctggtactgg tctatcgttt cctctttctc ttggtttcgg aggaccgcgg
     2461 gctattaagc tccgatccgc tctaccgcga gcattacagt gtcggtcggc tccgccgcct
     2521 tttggaccga cagagtgcct cgaccgaaca cgatgacctt tggtgctccc tccgcgtcct
     2581 ttggcttgtc ctggtcaagg accagccgca tcaggccctc ggcaacaaac cgctggccgc
     2641 cgccttgggg ttacccatcc tcaacgggca actgtttgat cgcctggacc tggacgcctg
     2701 ctggatcact aaccgcgatc tcctcgaggc cttctggtac ctggcctact accgcccgga
     2761 acccaacgcc ccgccgcggc gggtcaacta tgccgcgcta gacgtggaag agctaggctc
     2821 ggtctatgaa agcctgctcg actatcaccc ggtcatcacc tctgacggcc gcggccggct
     2881 caggttcgac ttgcatcccg gcttagagcg caagagcact ggttcatact acacccccgc
     2941 ggaactggtg gccgagctga tccgctccgc attggatccg gtgatcgcgg aacgtctgaa
     3001 ggccgcgaag acacccgagg aaaaagatag ggccctcctt tccatccgtg tttgtgaccc
     3061 cgcctgcggt tcggggcatt ttttgcttgc cgccgcccgt cgcctgggaa aggaacttgc
     3121 ccgcatccgc accggcgaag aggagcccgc cccggaacgc gtccgcgagg ccattcgcga
     3181 tgttgtttcg cactgcatct atggtgtgga caaaaatccg ctggccgtgg acctctgccg
     3241 tgtggccctc tggctggaaa gccacactgg cgacaagccg ctgacgttcc tcgaccaccg
     3301 catccgctgt ggcgactcgc tggtcggagt ttttgacctg tccgtgctcc ggcggggtat
     3361 tcccgacgaa gcctttgctc ccgtgaccgg cgacgataag gggtttgccc gcaccgtggc
     3421 caaacgcaat cgggaggagt gtcgcggcta ccgagacctg atccattgga a
//
LOCUS       DAOPTR010000337         3458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_21749_length_3458_cov_3.103144, whole genome shotgun sequence.
ACCESSION   DAOPTR010000337 DAOPTR010000000
VERSION     DAOPTR010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21749_length_3458_cov_3.103144"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1386
                     /locus_tag="PKI05_07475"
     CDS             172..1386
                     /locus_tag="PKI05_07475"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOA52081.1"
                     /translation="MSPTLEVSDMHLGRVIGRLLSAWHLGLAYGVLAVALLPGAALRG
                     GEAAQPVVEASVVEQVDVFISGEKGYHTYRIPSLLRSPDGSLLAFCEGRKHGLGDSGD
                     IDLVLRRLAPGQSTFGDLVVVWDDGPNTCGNPCPVVDQQTGVIWLWMTHNLGEDHERD
                     IVARKSRGTRTVWLAKSVDHGQTWTQPVNMTEHVKLPEWSWYATGPGCGIQTRSGRLV
                     IPCDHIADDGTWGSHVILSDDHGQTWRLGGVAKPKTNECEVVELSDGRLLLNMRNYNR
                     QFPCRAVAESSDGGITWSEVRYDQQLVEPVCQASIRRVDGITIGGRPLIAFSNPADTK
                     ERRRMTVRFSPDDGQTWPWSVVVWEGPAAYSCLASLPDGTVLLLYERGNKHAYERITL
                     ARIVIRVKSPGS"
     gene            1479..2927
                     /locus_tag="PKI05_07480"
     CDS             1479..2927
                     /locus_tag="PKI05_07480"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOA52082.1"
                     /translation="MLQRWFQLREHGTNVPREFLAGLTTFLTMAYIIVVQPQVLSGKM
                     FGNETGLDFGAVTTATCVSAAIATLIMGIYARYPIALAPGMGENFVFVLSLIPAAQVW
                     LEQEIQAGRIQAGTTTAWSIALGVIFYSGVLFLVLSLLGIRERLLEAVSPSMRNAIAA
                     GIGLFIALLGLQGASVIVGAPGQIVKLSPRLTSPDVIVFFVGLMVTAAFYARRIPGAI
                     VIGIFTATLLAVGLRQVIPLLPGLAETEIVRQSQLMTRFAPAEHLFSGPPSMAPTFAK
                     MDLIHALALPMIPYILIFLFMDVFDTLGTLVGVSEQAGFIRDNRIPRAKQALSADAVG
                     TVVGAVCGTSTVTSFIESAAGIEQGGRTGLTAVIVAILFLVAPCCYPLIEMVGSYPPI
                     TAAALLIVGSMMMKNVLKIEWDQATEAIPAFVTMTGIVFSYSIADGLALGFIVYPLIK
                     FLAGKGREVPWLMYGLAVVLLAYLVFAKAHMG"
     gene            complement(3137..>3458)
                     /locus_tag="PKI05_07485"
     CDS             complement(3137..>3458)
                     /locus_tag="PKI05_07485"
                     /inference="COORDINATES: protein motif:HMM:NF020317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HOA52083.1"
                     /translation="KSGVTLSLFTRSKAMATFVMLGKYSPEALSQISAERTEKFVKTI
                     TELGGSVRAMFATLGEVDLVLIVDLPGIEQAAKASVVVSKDTGIHFSTHPAIAVKDFD
                     QLVG"
BASE COUNT          675 a          908 c         1037 g          838 t
ORIGIN      
        1 cttgccctca ccccaaccct ctcccggagg gagaggggga cttcttggga tagaccctaa
       61 aggcaacgtg ccgcgccggt tgcaaacgcg tcgccttttc tccacaatgc ttcagggaat
      121 cggcgagatt atcgaaatcg ctttgatcgt ctgcacgatg tgtcccgtgt gatgagccca
      181 acgctggagg tttctgatat gcacctgggg agagtgatag gccgcttgct ctcggcatgg
      241 cacctcggat tggcctacgg tgttcttgcc gtagctcttt taccgggagc agcgttgcga
      301 ggaggcgaag ccgcacaacc cgttgtggaa gcctccgtgg tggagcaagt agatgtcttc
      361 atttctggcg agaaaggcta tcacacctac cggattccct cattgctccg cagcccggat
      421 ggtagcctct tggccttttg cgaaggccga aagcacgggc taggcgattc gggcgacatc
      481 gaccttgttt tgcgacggct ggccccgggt caatcaactt ttggggacct cgtcgttgtc
      541 tgggacgacg gtcccaacac ttgcggaaac ccctgtcccg tggtggatca gcaaaccggt
      601 gtcatttggc tttggatgac gcacaatttg ggcgaggatc atgagcgaga catcgtcgcc
      661 cgcaaaagcc gtggcacacg cacggtgtgg cttgccaaga gtgttgacca cggccagaca
      721 tggacccagc ctgtcaacat gacggagcac gtcaagttgc ctgaatggtc gtggtacgcc
      781 accggacccg gttgtgggat tcaaactcgc agcggtcgtt tggtcatccc ctgcgatcat
      841 attgcggacg acggcacgtg gggttcccac gtcatcttga gcgatgatca tgggcaaacg
      901 tggcgtctgg gcggagtggc taagcccaag acgaatgaat gcgaggtcgt cgagctttcg
      961 gatggccgac ttctcctcaa tatgcgaaat tacaaccggc agtttccttg tcgcgctgtg
     1021 gccgagagtt ctgatggggg gatcacctgg tccgaggtac ggtatgacca gcagcttgtg
     1081 gaaccggtgt gtcaagccag tatccgccgc gtggacggaa ttactatcgg agggcgaccg
     1141 ttgatcgcct tttccaatcc ggcggacacc aaggaacgcc gcagaatgac ggtccgcttt
     1201 agcccggacg atggtcagac ctggccatgg tcagtcgttg tttgggaagg tccggcagct
     1261 tacagttgtc tggccagttt gccggatgga actgtccttc tcctttatga acgtgggaac
     1321 aaacatgctt atgagcggat caccttggcc cgaattgtga ttcgggtaaa atcgccaggc
     1381 tcatgaacat cgagatggcc tgcggtaaat gtgatctggg gaagcgaaga ccttcttggg
     1441 gggcagcata agtatgtcag ttgaccggag gttgaggcat gttacaacgt tggtttcagc
     1501 ttcgagaaca cggaacgaat gtaccaagag aattcctggc aggtctgacg acctttctca
     1561 cgatggccta catcatcgtg gtgcaaccac aggtcctctc cgggaagatg tttggaaatg
     1621 agacggggct ggattttggg gccgtgacaa cggccacgtg cgtttccgcg gccatcgcca
     1681 cgttgatcat gggaatctat gcccggtatc cgattgccct ggcgccgggc atgggagaga
     1741 atttcgtctt tgttctctcg ttaataccgg ccgcccaggt ttggttggag caggagattc
     1801 aagcggggag aattcaggcg ggaacgacca cggcttggtc tattgctttg ggggtcattt
     1861 tctattccgg ggttttgttt ctcgttctat cgctactcgg aattagagaa cgattactcg
     1921 aggccgttag tcccagcatg agaaatgcca ttgcggcggg gattggcctt tttatcgccc
     1981 tgttggggct gcaaggggca tcggtgattg tgggggctcc ggggcagatc gtcaaattga
     2041 gtccgcgatt gaccagtccg gatgtgatcg tcttttttgt gggtctaatg gtgacggcgg
     2101 cattctatgc ccggcgcatt cccggggcga ttgttattgg tatttttacg gcaactcttc
     2161 tggctgtggg attgcggcag gtgattccac tactaccggg actggccgaa acggagatcg
     2221 tccggcaatc gcagcttatg acccgattcg ccccggcaga acatttgttt tccggtccac
     2281 cctcgatggc cccaacattt gcgaaaatgg atttgatcca tgcccttgct ttgccaatga
     2341 tcccctacat tttgattttc ctattcatgg atgtgtttga cactctagga accttagtgg
     2401 gtgttagcga acaggccgga ttcattcgcg ataaccgcat cccccgggcg aaacaggcac
     2461 tgtccgcaga tgccgtggga actgtggtgg gcgccgtgtg tggaactagt accgtcacaa
     2521 gctttatcga aagtgcggcg gggatcgagc agggcggaag aacaggcctt acggcggtga
     2581 tcgtggccat cctctttctt gtggccccct gttgttatcc cttgatcgag atggtcggca
     2641 gttatccgcc gattaccgct gccgcccttt tgatcgttgg gtcaatgatg atgaagaatg
     2701 ttctcaaaat cgagtgggat caggccaccg aggcgattcc cgccttcgtc accatgacgg
     2761 gaattgtctt cagctattcc atcgctgacg gcttggccct ggggttcatt gtttatcccc
     2821 tcatcaagtt ccttgccggc aagggacgcg aagtcccttg gctgatgtat ggcctggctg
     2881 tggttctgtt ggcctatctt gtgttcgcca aggcacacat gggttaatgc tcggacgatg
     2941 ccgggttggt gatccccgga tgcttgcccc catagtccac gggtggcggc aatggttatc
     3001 gtttggaagc aatgggcgaa gctggcaagt taccggctgg cgacataacg ccctgttccc
     3061 cgacgtacgg cttcgtcaaa tgctcgtagg cctccgcggg gtcatgttct ggggccaaaa
     3121 aggactagcg gcacggttat ccaaccagtt gatcgaagtc cttcacggcg atggcgggat
     3181 gggtgctgaa gtggatgcct gtgtctttac tgacgaccac tgaggcctta gcggcctgtt
     3241 cgattcccgg aaggtccacg atcaggacga gatccacctc tcccagcgtt gcgaacatcg
     3301 cccgaacgct gcctcccagt tccgtgatcg tcttcacgaa cttttccgtg cgttcggcgc
     3361 tgatctgact caaggcctcc ggggaatact tgccgagcat gacgaaggtg gccatagcct
     3421 tactccttgt gaaaagagac aacgtcacac cagacttc
//
LOCUS       DAOPTR010000338         3456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_21770_length_3456_cov_2.798883, whole genome shotgun sequence.
ACCESSION   DAOPTR010000338 DAOPTR010000000
VERSION     DAOPTR010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21770_length_3456_cov_2.798883"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PKI05_07490"
     CDS             <1..181
                     /locus_tag="PKI05_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52084.1"
                     /translation="EIYLYLREDRPSCFPWEQVVEKPTSPQDTPGPSEVTDAVISLMD
                     TTQVPPGQPLYDQGQ"
     gene            268..1158
                     /locus_tag="PKI05_07495"
     CDS             268..1158
                     /locus_tag="PKI05_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008663627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOA52085.1"
                     /translation="MHRQGENRPEQEVVAHDLLEDLPVRRLTHKNFTIEGYSRAAFQT
                     YWRIPEFRLGFDLGGQPWSFMGTPSWFVSHTHIDHFVALPAYVSRRRMMKMEPPTIYL
                     PEPCVPLVERLLHLVSRLDRGRLPCQLVPVTPGQEFELSRELVVSSLATQHTVPSLGF
                     IVWERRKKLKPEFTGLPGEKIRDLRLSGVEVTNEIRFPRVAYLGDTRIDVLDEYPDLY
                     RAEVLIMEVTFVSPRHRGEKIRKFGHVHIDDIVARRDRFQNEVIIASHFSIRYTRKLV
                     ERLVRKALPDLLDGRLQVWL"
     gene            complement(1250..3157)
                     /locus_tag="PKI05_07500"
     CDS             complement(1250..3157)
                     /locus_tag="PKI05_07500"
                     /inference="COORDINATES: protein motif:HMM:NF027585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4914 family protein"
                     /protein_id="HOA52086.1"
                     /translation="MAIDLESEIGSRLAGPPALLEVLREAPRVLIPEKTSELFDLAVG
                     GPDQDYFEVAYDVPGMGSVVEAIVVRCRNGVAVNYPDPYMRRRDPDCLVVADDRPTDK
                     PRFCERFSIPFEQARAEILGWLKRQQLVVVPFFAGGKRWGFPAALVAPMNASFFAAGL
                     ALLQEMIPAGELRQDFSPQAVVYLAPPFRHTLCEGKQVVIHHRGPPVYEVFSLNLYPG
                     PSAKKGVYGVLLSLGEEQGWVTLHGSTVQVMTPYDNIMTIMHEGASGGGKSEMLEYPH
                     REPDGRLLLGQNVVTGQKRFISLIQGCTLFPVTDDMALCHPELQNTRGRKLVVSDAET
                     AWFVRVDHIREYGTDPYLERICTMTKEPVVFLNIHAVPRATCLIWEHVEDAPGKPCPN
                     PRVILPRHLVPNVINGPVAVDVRSFGVRTPPCTKEHPSYGILGMMHVLPPALAWLWRM
                     VSPRGHANPSITESDSLQSEGIGSYWPFATGCRVDQANILLRQMLETPETRFVLVPNQ
                     HIGAWKVGFMPEWLVREYLARRGGARFRPEQLKPARCPLLGYALSFMQIEGTQLPSWF
                     LEVDTQPEVGPEAYDQGAEILYRFFHKALTELAGMPGLDPLGRRIIRACLERAPLSTY
                     EALIPFNPYAS"
BASE COUNT          775 a         1067 c          907 g          707 t
ORIGIN      
        1 cgagatctat ctctacttac gggaggatcg gccaagttgc ttcccctggg agcaagtcgt
       61 cgagaaaccg acatcccccc aagacacacc ggggccaagc gaggtcacag atgccgtgat
      121 ctctctgatg gacaccacac aagtgccccc tgggcagcct ctctacgatc agggacaata
      181 acgtttcaat cggtcccggt gggaagacct ggaagcttca tggccattag gtgggagtct
      241 ttgcggtttg tgactgctgg aaatgagatg catcgacagg gtgaaaatcg tccggagcag
      301 gaagtcgttg cccatgacct cctggaagac cttcctgtac ggcggttgac ccacaagaat
      361 ttcacgattg agggctattc gcgggcggca ttccagacgt attggcggat tcccgagttc
      421 cgtctaggat ttgacctcgg cggccagcct tggtccttta tggggacgcc atcctggttt
      481 gtgtcgcaca cccacattga tcactttgtt gccctcccag cctatgtgtc ccggcggcgg
      541 atgatgaaaa tggagccacc caccatctac ctgccggaac cttgcgtccc tttggtggaa
      601 cgactcctcc atctggtcag ccgcctggat cggggacgcc ttccctgcca gcttgtgccc
      661 gtgacacctg gtcaagagtt cgagctttcc agggaactgg ttgtcagcag cctggccacc
      721 cagcatacgg tcccatcctt ggggttcatt gtctgggaac gaagaaagaa actcaaaccg
      781 gagtttaccg gccttccggg cgaaaaaatc cgggacctac ggctaagcgg cgtggaggtc
      841 accaacgaaa tccgtttccc ccgcgtggct tacctgggcg atacacggat cgatgtcctc
      901 gatgagtatc cggaccttta tcgcgccgag gtcctcatca tggaagtcac gtttgtctcg
      961 cctcgtcatc ggggagaaaa gatccgcaaa ttcggtcatg tccacatcga cgacatcgtc
     1021 gcccgtcgag accgcttcca gaatgaagtc atcatcgctt cccacttcag catccgctac
     1081 acccggaagc tcgttgaacg gttggtccgc aaggccctac ccgacttgct cgacggtcgc
     1141 cttcaagtct ggctttaatc tttgataacg agcgatcagc atggcttcgt tcaccgccaa
     1201 gagccgctgc ttccaagaca gattccaccg ccgcccagtc aaaggaccat cagcttgcgt
     1261 agggattgaa cgggattagg gcctcgtatg tgctcagcgg tgcccgttcc aaacaggcgc
     1321 gaatgattcg ccgaccgagg ggatccaatc ccggcattcc cgccaattcc gtcagtgcct
     1381 tatggaaaaa gcggtaaagg atctctgccc cctggtcgta ggcctctggt ccaacctccg
     1441 gctgggtatc gacttccaaa aaccaactgg gaagttgcgt gccctcgatc tgcatgaagg
     1501 aaagggcata gcccaacaac ggacaccgag cgggtttgag ctgctcgggc cgaaagcgtg
     1561 cgccgccacg ccgggccagg tattcccgga ccagccattc gggcatgaat cctaccttcc
     1621 atgcgccaat gtgctgattg ggaaccagga caaacctcgt ttctggcgtc tccaacatct
     1681 gacggagaag gatgttcgcc tgatcgacgc ggcaacctgt ggcaaatggc cagtatgagc
     1741 caattccttc gctttgcagc gaatcgcttt ctgtaatgct ggggttggca tggccccggg
     1801 gagaaaccat ccgccacaac cacgccaagg ccggtggcaa gacatgcatc atccccaaga
     1861 tcccgtagct gggatgctct tttgtgcacg gcggcgtccg aaccccaaag cttctcacat
     1921 ccaccgccac cggtccattg atcacattgg gaaccaaatg gcgaggcaga atgacgcggg
     1981 gattggggca cggcttaccg ggggcatctt ccacatgttc ccagatcaaa caggtggccc
     2041 gcggcacggc atgaatgttc aagaacacca ccggttcttt ggtcatcgtg caaattcgtt
     2101 ccagataggg gtccgtgccg tattctcgaa tatggtccac ccgaacgaac caagcggttt
     2161 cggcatctga aacgacgagc tttcgccccc gggtattctg gagttccgga tggcacaacg
     2221 ccatgtcgtc cgtgacagga aagagggtac acccttgaat caacgaaata aaccgcttct
     2281 gtcccgttac gacattctgg ccaagtaaca accgtccatc gggttcacgg tggggatatt
     2341 ccagcatctc gctcttcccg ccaccgcttg cgccctcgtg catgatcgtc atgatgttat
     2401 catagggcgt catgacctgg acggtggagc catgcaacgt gacccaacct tgctcttcgc
     2461 ccaacgagag cagcacccca tagacgccct ttttggcgct agggccggga tacaagttga
     2521 gcgagaaaac ctcgtacacc gggggaccgc ggtggtggat caccacctgc tttccttcac
     2581 acaaagtgtg tcgaaatggc ggcgctaaat aaaccaccgc ctgtggtgaa aaatcttgcc
     2641 gcaactcccc ggcgggaatc atctcctgca acaaggccag gccagcggcg aagaaacttg
     2701 cgttcatcgg cgctaccaac gcggcgggaa agccccatcg ctttccaccc gcaaaaaagg
     2761 gaacgaccac caactgctgc cgcttgagcc aaccgagaat ctccgcccgg gcctgctcga
     2821 agggtataga gaatctctca caaaaacgcg gtttgtcggt cgggcgatcg tccgccacga
     2881 ccaagcaatc ggggtcccgc cgtcgcatat aggggtcggg ataattgaca gccacgccat
     2941 tgcggcaacg gaccacaatg gcctccacca cggaacccat ccccggcacg tcgtaagcta
     3001 cttcaaaata gtcttgatcc ggaccaccca ccgcgaggtc gaacagctcg ctcgtcttct
     3061 cgggtatcaa aacgcgcgga gcctcgcgca gcacctcgag cagcgccggc ggtccggcca
     3121 accgactccc gatttcactc tccaagtcaa tcgccatacg cgttcctcga tacgtaacgt
     3181 ctatcccaag gcatgttacg gaactgtcgc ttcaaagcaa accgcacacc ggcaacgtcg
     3241 cccgcatcgg caacgcttga actattcgcc aattaaccca accggtcaac ctcatccgga
     3301 cgagtgtggg gcgtcgtact atcttggtca taccacctca gatatattcg attttaacca
     3361 tttgtgggca ctccaggacg acaccaccca aatttattag tacctatggt ccatcataat
     3421 ccccccatag atgctaatac cgctacgagg tcctag
//
LOCUS       DAOPTR010000339         3455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_21774_length_3455_cov_3.206471, whole genome shotgun sequence.
ACCESSION   DAOPTR010000339 DAOPTR010000000
VERSION     DAOPTR010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21774_length_3455_cov_3.206471"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PKI05_07505"
     CDS             complement(<1..476)
                     /locus_tag="PKI05_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52087.1"
                     /translation="MIPGWFGFYNRHGVSGSSPVGRGDLLPAFKVFLAAIVIISFLAQ
                     SASEVRAEASPQSNRPVSLQVPQSVPLQPDQWTSPEGLSSALQILLLLTVLSLAPAII
                     LMTTSFIRVVVVLSLLRQALGTQTLPPSQVITGLAIFITLFVMSPVWMRVYDEAVV"
     gene            complement(593..1441)
                     /locus_tag="PKI05_07510"
     CDS             complement(593..1441)
                     /locus_tag="PKI05_07510"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOA52088.1"
                     /translation="MPGLTWHRYLFLFFVAVIVTHPGVCRAQEATGRPWSGSGFVESQ
                     ATNGGMSFYQSANTNLSGFPAGDQVRQGEAGGDWPAAVPSNTPGFAFAGRQELTSPGR
                     EHPRTSATTPADGPVSDPVSPPQAGASSRASETSIPLRHPDAKGTSQTSQAKSPTAFG
                     SATAPWMTTISALAIVLGVLFLFYWGMKRLGPKTNQILPAEAVELLGSTALSPRQRLC
                     LIRVGRKLVLVAMSSEGVEPLTEIDDPVEVDRLVGICASQRNGSATQVFRQVFSQYID
                     NAPRTG"
     gene            complement(1609..2304)
                     /gene="fliN"
                     /locus_tag="PKI05_07515"
     CDS             complement(1609..2304)
                     /gene="fliN"
                     /locus_tag="PKI05_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HOA52089.1"
                     /translation="MSGDQLSQAEIENLLKAATQGAKPGGAGSQAGGSGTTESATGMP
                     SSSDSGGALSQDEIEKLLQQAGRGTGTVTPPDPKEKGSPSTAERSEQQPHAEIHPADL
                     DYLFHQAEQALASLENEGLPGLPPGVSAFQFTEFTGAPPSTQAATLDLLKEVELDVRI
                     ELGQTRMYLEDVLRLRKGTVVTLDKLAGDPVDIYVNGRLVARGEVLVLNDNFCIRVAE
                     LIAGKEPHVKDDA"
     gene            complement(2334..2975)
                     /locus_tag="PKI05_07520"
     CDS             complement(2334..2975)
                     /locus_tag="PKI05_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HOA52090.1"
                     /translation="MVNSSAPNAKATPNSDPGNQSSTESRPSNGKGFGGHANRLKLIG
                     FVLGLVTVEAVAAYFILPTSSDAKSPAQEGVAETAGIPGAPSPISVPETQLDPTQGLG
                     NTPGPDDVEVDLGQFSITAYQPTSQTTLRIDFHLWGTVDRSLDQEFQAAWAKNINRLR
                     DQIIVIVRSADLSDLTDAGLGLIKRKILEKVNHTLGKPYLRTVVFSEYSFLEQ"
BASE COUNT          778 a          972 c          957 g          748 t
ORIGIN      
        1 tgcacaacgg cttcatcata gacccgcatc caaacgggcg acatgacgaa gagcgtgata
       61 aaaatcgcta ggcccgtgat gacttggctt ggcggcagtg tctgcgtccc caaggcttgt
      121 cgcagaaggc tgaggacgac caccacgcgg ataaatgatg tcgtcatcag aatgatggcg
      181 ggcgccagac taaggactgt cagaaggagc aaaatctgga gggcactcga gaggccctcc
      241 gggctggtcc actggtcggg ttgcaatggt acagattgag gaacttgcaa cgagacgggg
      301 cgatttgatt gaggcgatgc ctcggccctg acctctgatg ctgactgggc caaaaaggag
      361 atgataacga ttgctgcgag aaaaacctta aaggcgggca ggaggtcgcc acgtcccacg
      421 ggcgaggaac cacttacgcc gtgccggtta tagaaaccga accaccccgg aatcatcgca
      481 tgcttcccat ccttgggtat ctcatgctct ccacacaatt ccaaacgtct ttgtcagtaa
      541 aacccttgtg tgacaggtac agtccgccct ggagcgcctg tgccgccaat gcctagccag
      601 tcctcggggc gttatcaata tactgggaaa aaacttgacg aaagacttgc gtggcactgc
      661 cgttccgttg cgacgcgcag atacccacca gtcgatcgac ttccacaggg tcatcgatct
      721 cggttagggg ttcaacaccc tccgaactca tcgccaccaa cacaagtttt cggccaacgc
      781 gaatgagaca caacctctgc cgcggcgata gcgcagttga ccccagtaat tcgacggctt
      841 cagcagggag tatctgattg gtctttggcc ctagtcgctt catgccccaa taaaaaagga
      901 acaatacacc aaggacaatg gcaagggcac taattgtggt catccaaggt gccgttgcag
      961 atccaaaagc ggttggggat ttggcctgcg acgtctggga agtccccttt gcgtcggggt
     1021 gccgcaaagg aatcgacgtc tctgacgcgc gtgagcttgc accagcctgc ggaggtgaca
     1081 cgggatcgga cacagggccg tctgctggag ttgtcgcgga ggtacgtgga tgttctctgc
     1141 caggtgaagt caattcctgt cttcccgcga aggcaaagcc cggcgtgttg gagggaacag
     1201 ccgccggcca atctccgcct gcctccccct gcctcacctg atcaccggcg ggaaagccgc
     1261 tcaagttcgt gttggccgat tggtaaaaac tcattccccc attcgtggcc tgggattcca
     1321 caaaccccga ccctgaccaa ggtcgtcccg tcgcctcttg agcgcgacac accccaggat
     1381 gcgtgacaat tacggcaacg aaaaacaaga acaggtaacg atgccaggtc aacccgggca
     1441 agaaagacgg catccttatg aggggctttg aggaagaaac gggttccctt cggtatgcct
     1501 tggcaacacc atgtcgaggc cccttggaat aagaggccca aaaggcaccg ttgtcgttaa
     1561 ccagcatccg tgctgccacg gaaaaacccc ttgtcgtctt atcgaggatt aagcgtcatc
     1621 tttcacatgc ggttctttcc cggcgatgag ctccgccacg cgaatgcaga agttgtcgtt
     1681 gagaacgagg acttcgccgc gggcaacaag ccgtccgttg acataaatat ccacagggtc
     1741 acccgccagc ttgtccaacg tcaccacagt tcccttccgc agacgcagca cgtcctccaa
     1801 atacatccgc gtttgtccca attcgatgcg aacatcaagt tcgacctctt tcaaaaggtc
     1861 cagggtagcg gcctgggttg aaggtggggc accggtgaac tccgtgaact ggaaggccga
     1921 aactcccgga ggaagccccg ggagaccctc gttttccagc gatgccaggg cctgctcggc
     1981 ctgatgaaag agatagtcaa ggtcggccgg gtgaatctcg gcgtgcggct gttgttcaga
     2041 tctctcagcc gttgaaggac tacccttttc tttgggatcg ggaggggtga ccgttccggt
     2101 cccacgtccg gcctgctgga gtaatttctc gatctcgtcc tgactgagtg ctcccccgga
     2161 atcactgctg gaaggcattc ccgtggccga ttccgtggtg cccgaaccgc cggcttggga
     2221 gccggctccc cctggctttg ctccctgcgt cgccgccttg agcaagtttt caatttcggc
     2281 ctgtgacagt tgatcgccag acatgggact cccttccctt gccaacttag ccgtcactgt
     2341 tctaagaagg aatactcact gaacacaacc gttcgcagat agggctttcc tagcgtatgg
     2401 ttaactttct ctaaaatctt ccttttgatc aaccccaaac ctgcatccgt gaggtccgat
     2461 aggtcggcac tgcggacaat cacgatgatt tggtcgcgca gccggttgat atttttcgcc
     2521 caagccgctt ggaattcctg atccagagat ctgtcaaccg tcccccataa gtggaagtcg
     2581 atccggagtg tggtttggga cgttggctgg taggccgtta tgctaaattg acccaggtcc
     2641 acttcgacat cgtcaggacc tggggtatta cctaacccct gagtgggatc caactgagtt
     2701 tcgggaaccg aaatcggcga cggcgccccg ggaatccctg cggtttctgc caccccctcc
     2761 tgagcaggag attttgcgtc actggaggtt ggcaaaatga agtacgcagc cacggcctcc
     2821 acggtgacta gcccgaggac aaatccaata agtttcagac ggtttgcgtg accgccaaag
     2881 cctttcccat ttgatgggcg gctttcagta gagctttgat taccgggatc actgtttggg
     2941 gtagccttcg cattaggtgc agaactattt accatgctcg ccctttccga attgcgcgga
     3001 aactgaatag tcgctgtgac aagccaaagc gggggtgacc aagtaccacc gcctcaattc
     3061 aaatataggt catgcttttg cgcggggtaa aatgcgcatg ccgggaaata tgcaccgctt
     3121 aaggaggttt cacatacatg agaagcagca taaattagag aactcaatct ggctgggtac
     3181 ctttcaacgg ggcacagtga accgggctat tgatcgggct accggcccca tcgtcgagaa
     3241 aaggtttggc tttgtgggga ttcttacggc gaaagacgat cgccatcagg gcgtggaaga
     3301 gcccttcaag tctcttgcca gttcgttgag gagggaaact tcgacccttg cattttgtgg
     3361 ccgcttttcg ggatcgggat cggtccaaag cggctcattc gcagctgcta ttgaaatacg
     3421 gattcgctca ggctcgatgc ccgccgctat caagc
//
LOCUS       DAOPTR010000340         3446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_21842_length_3446_cov_4.189325, whole genome shotgun sequence.
ACCESSION   DAOPTR010000340 DAOPTR010000000
VERSION     DAOPTR010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21842_length_3446_cov_4.189325"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(396..1883)
                     /locus_tag="PKI05_07525"
     CDS             complement(396..1883)
                     /locus_tag="PKI05_07525"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOA52091.1"
                     /translation="MTLRTKQTMSGVRRGLVGWLFLVASGISAWASAADLIHPQRLTP
                     YGLEVAWFQQVQVDPARGRVTEWLLDRGTLFVVTNTAVLQAFDAETGRSLWATPVGRR
                     ELLTLKPTAGEHLVAVVNGSTIYVLNRYTGVILWSRQTEGAVGAGPVLAPTHVYVPLI
                     RGKIEAYRLEWANPETALAARTAEFPQDQATSPATAGMTTQSSEQSNGAESVTPIAMK
                     PVAKEEFRLSTQPRPGHFVMSWGRALVEPRLTFADRNRERLAWPTDKGLLFVGELDVR
                     SELRFSVQYFFQISGGDIVAPPCIRPGDPSNPADIQRMLYVAATNGFVYALREWDGKL
                     LWRFSSGEPIKESPALIGDDLFVTTQTGGMYRLNAVSGDLIWFARDLRRFVSASDQRV
                     YAVDGLNNLVIVDLQTGSRVGQLDVSRYPLRYANSETDRIYLASDTGLIQCIREARLP
                     QPIKHVVPITPLKEQKPAKPAAAQPAETPAQPQPPPQPAGENPFQ"
     gene            complement(2113..2907)
                     /locus_tag="PKI05_07530"
     CDS             complement(2113..2907)
                     /locus_tag="PKI05_07530"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOA52092.1"
                     /translation="MYICTCQRASYIKGGNAMGPNAVIGEFAGQIAAELDEFCRSTVE
                     RVCGTEYPDDMFRLADRLGMEIIVDGGESHRARCLRIQRGEKSQWLLIVRDEPRWERM
                     QWAIAHELGESLAGQAIMKLGWDVEDLQPVHREWLANEIAKRLLLPFPEFLSEGAALE
                     WDLYRLKEQYPNASHEVIARRMLDAPRPIVITIFDNGRLYWRKSNSLIASPPLTQQEK
                     ACWRAAHETGQSVRYQNAVESIVCWTIHENGWQREILRTESPWLEE"
     gene            complement(3051..>3446)
                     /locus_tag="PKI05_07535"
     CDS             complement(3051..>3446)
                     /locus_tag="PKI05_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52093.1"
                     /translation="KGQERVKELNARFAEWYYIISDDVYRKIHLGIGDIVKKKEKKEE
                     ETKTSDSSTPSPTEESKKAEATSSGDTSQAAKESPQETKPVESTPSPEAPAGASPPAP
                     PSEETAPATGAAEQQPQGGDSSPEPMPMQ"
BASE COUNT          733 a         1021 c          926 g          766 t
ORIGIN      
        1 aatgttacag aatacacgcc cgctgatacc cgcaaataag gacctatccc ataatctcct
       61 ctcgcccgtt cggcgggaga ggggtcgggg gttaggggtg gctcgaatcg ctgcggcttt
      121 tctcgattga cgccgacgat cgcttgccct caccccaacc ctctcccgga gggagagggg
      181 gtactttttg ggataggttc taaatccctt gccgcgtgaa gccgtggccc gattcgccct
      241 tggacgcaag gcccaggctc ggtcgctttt tttacgccac tcccacttcc taccaagttt
      301 gctccgcgca caaaaaatat gtcgctttcg ggtgaacgaa acccgttggc gagcaagaac
      361 ccagagatag taggcggatc aatgatgacg cctcatcatt gaaaaggatt ttcccccgcg
      421 ggttgcggag gcggttgagg ctgtgcggga gtttcggccg gttgcgctgc agctggtttt
      481 gcgggtttct gttccttaag cggagtgatc ggcaccacgt gcttgatcgg ctgcggcaag
      541 cgagcctcgc gaatacactg gatcaggcca gtgtcgctgg ccaggtaaat ccgatccgtt
      601 tcggagttcg catatcgcag cggatagcgt gacacatcca actgcccgac cctcgaacct
      661 gtttgaagat cgacaatgac aaggttgttg agaccgtcca cggcgtatac acgttgatcg
      721 ctggccgaga caaaacgtcg gagatccctg gcaaaccaaa tcagatcacc agaaaccgca
      781 ttgaggcgat acattccacc cgtctgggtc gtgacgaaca ggtcgtcgcc gatcagagca
      841 ggtgactcct tgatgggttc acctgaggaa aaccgccaca gcagtttgcc gtcccattcc
      901 cgcaaggcat aaacgaatcc gtttgtagcg gccacataga gcattcgctg gatgtctgcc
      961 ggattggaag ggtcacctgg acgaatacat ggtggcgcca caatatcacc gccgctaatt
     1021 tgaaagaaat actgcacaga aaaccgcaac tcggagcgca catccagttc gcccacaaag
     1081 agcaaacctt tgtccgtagg ccacgccaac cgttcccgat tacggtccgc aaacgtcagc
     1141 cttggctcga cgagtgcccg gccccatgac atcacgaaat ggccaggccg tggttgggtc
     1201 gataatcgaa actcttcctt ggccacgggc ttcatagcta tcggcgttac ggattcagca
     1261 ccgttagact gttcggaaga ttgggtcgtc atgcccgcgg tcgccggaga tgttgcctga
     1321 tcttgaggga attccgcggt cctggcggcg agggccgtct ccggattggc ccactccaaa
     1381 cggtacgctt caatcttgcc gcggataagg ggcacgtaaa catgcgtcgg cgcgaggacc
     1441 ggccccgctc cgactgcccc ctcggtttgg cgactccaaa ggatgacccc tgtataccgg
     1501 ttgagtacat aaatcgtgct gccgttgacc actgcgacga gatgctcccc tgccgtcggt
     1561 ttcagggtca gcaactcgcg tcgccccacc ggcgtagccc agagggatcg cccagtctcg
     1621 gcatcgaagg cctgtaacac ggcagtgttc gtgacgacaa acagggttcc tcgatcgaga
     1681 agccattccg tcacacgtcc ccgagcagga tcaacctgga cctgctgaaa ccaagcaact
     1741 tccaagccgt aaggtgttaa tcgctgggga tgaatcaaat cggcggcgct tgcccaagcc
     1801 gaaatcccac tagcgacaag gaaaagccat ccgacaagcc ccctgcgaac gccggacatc
     1861 gtctgttttg ttcgtagcgt catggttctg cctcgaatca ctgagcatga taatgaccac
     1921 cacgagcatc cgtgccctac agcttttcgg ccgcgtcaga atatataggc ggcattccgc
     1981 aacgggatcg cccccatgct gggaatatcg ctatcttcta tgataatcgc ccccaatcca
     2041 aaagaaactc atttccgcca cagtttgccg agtttatcgc gagaagcatg accgtcgctt
     2101 caccggggcg ggtcattcct ccaaccaggg cgattccgtc cgcagaattt cccgctgcca
     2161 accgttctcg tggatggtcc agcaaacgat cgattccacg gcgttttgat aacgaacgga
     2221 ctgtcccgtt tcgtgcgcag ctcgccaaca ggccttttcc tgctgcgtga gaggcggtga
     2281 tgcgatgagg ctattgctct ttcgccagta caacctgcca ttgtcaaaaa tcgtgatgac
     2341 aattggccgg ggcgcatcca acatgcggcg cgcgatgact tcgtgactgg cattgggata
     2401 ctgctctttc agccggtaca agtcccattc aagcgcggca ccctcggaca aaaattccgg
     2461 aaagggcaag aggagccgct tggcgatctc gttcgccagc cactctcgat ggacgggctg
     2521 aagatcctca acgtcccagc ccaacttcat gatggcctgg ccagcgagtg attctcctaa
     2581 ctcgtgggcg atggcccatt gcattcgttc ccagcgcggt tcgtctcgga caatcaagag
     2641 ccattgcgat ttctcccctc gttggattcg cagacatctc gcccgatggc tctccccgcc
     2701 atcaacgata atttccatgc ccagccggtc ggctagccga aacatatcgt ctgggtattc
     2761 tgtcccacag acgcgttcaa ccgtcgaacg acagaactcg tccaactctg cggcaatttg
     2821 accagcaaat tccccaatga cggcgtttgg tcccatggca ttcccccctt tgatgtaact
     2881 ggcccgctga catgtacaaa tatacactat tatggcacct ggtggtcaac cgtcaagttg
     2941 cggggggcgg gtatgtatga gcgggataca agccctaaaa aacaaaaaag gcgttggggc
     3001 gatgccaacg ccttttttgt gatgcgatcg acgcagacag aatcttagca ttactgcata
     3061 ggcatgggct ccggtgaact gtccccgcct tgtggctgtt gttccgcagc acccgtcgca
     3121 ggtgccgttt cctccgacgg tggtgcaggc ggagaagcac cagccggtgc ctctggggat
     3181 ggtgtggact cgaccggttt ggtctcttgt ggcgattctt tcgccgcttg agacgtgtcg
     3241 cctgaggagg ttgcctcggc cttctttgat tcctcagtag gcgacggagt acttgagtcc
     3301 gatgtcttcg tctcttcctc cttcttttcc tttttcttca cgatgtcacc gatccccagg
     3361 tgaatcttcc gatagacgtc gtcggaaatg atatagtacc attcggcaaa tcgagcattt
     3421 agctccttaa cccgctcttg cccctt
//
LOCUS       DAOPTR010000341         3444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_21858_length_3444_cov_3.793744, whole genome shotgun sequence.
ACCESSION   DAOPTR010000341 DAOPTR010000000
VERSION     DAOPTR010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21858_length_3444_cov_3.793744"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1166
                     /locus_tag="PKI05_07540"
     CDS             147..1166
                     /locus_tag="PKI05_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52094.1"
                     /translation="MAEHGNRRQFLNYGLLGAMGAAAASSLEERILLAALQEGAPEKA
                     AQTPVDDRPMPCGRIRNLTISRMLIGGNLIGGWAHSRDLLYVSRLFKAYNTEERIFQT
                     LALCESVGINTVQLDPACINVFHRYLKERGGKMQALVCIHPDADAGKVRSQIEDLLNK
                     GATFLYTHGEVTDRHTMAGRLDILAKTMELVKEAGVPAGIGSHSLETPMQCEAQNVGA
                     EFYVKTFHPDNYWSATPPEKRQEWCWYLPRGQEPGTYHDNIWCLDAEKTAEFFKTVKK
                     PWFAFKVLAAGAIHPRVGFSYALRNGADFLIVGMFDFQVAEDVTIFKDAYYKLGQQRQ
                     REWYA"
     gene            1356..2039
                     /locus_tag="PKI05_07545"
     CDS             1356..2039
                     /locus_tag="PKI05_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOA52095.1"
                     /translation="MALVTLRIVDGVDRGRVYEDIPTPITVGREEGNVVRLSDERVSR
                     FHLKIQEDRNRLVLTDLASTNGTRVNGEPVQVAQIRPGDIITIGRTVILVGSREQIAQ
                     RLAAIRKKKEELGETGDIEISPEVVSPWFSPSLERELDWSEQSNVLRPIHELVPPDLP
                     EDLSPAQLAQLTELLQYFHIRLRRLVQAASMDHASESVTLEQRHWQGLLDLYDRVAHY
                     LRMIGEPPT"
     gene            2227..>3444
                     /gene="dnaE"
                     /locus_tag="PKI05_07550"
     CDS             2227..>3444
                     /gene="dnaE"
                     /locus_tag="PKI05_07550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOA52096.1"
                     /translation="MATPPFVHLHCHSHYSLLDGAGTVDRLLDRARDLCMTALALTDH
                     GNLFGALEFYQKAKDRGIKPIVGYEAYVAPGSRFQREAASMKEASYHLTLLAQDKRGF
                     QNLLRLASAAYLEGFYYRPRIDKELLAQHHEGLICLSGCPSGELSRTLLAKEDPDIAR
                     ACEVADWFRSLFGDRYFIEIQDNGTEIQRLAMKGAIAVAKKMNIPLVATSDVHYVMPE
                     DADAQDVLLCINTGKFRSDTNRMKMDGREYYLRSPEQMYQVFSGLEEAVRRTQDIASM
                     VDLELELGRRHFPIYTPPDGKTAEQYLRELCIEGLKERYRHDPRRFRNGELAPEVMER
                     LEKELYVINTLGFANYFLIVWDFVRFARSKGIQATARGSGVGSLVSYALKLSHVCPIE
                     YGLLFERFLDLNRR"
BASE COUNT          764 a          928 c          978 g          774 t
ORIGIN      
        1 tgacagcgca aattgtcaaa aattacaatc gagctgaagc gaagaattga aacaacagcc
       61 gggcaagagc ctctggcaga gtcaggtttt atttctctcc gttttcaatt tgggcggtcc
      121 aattaatcaa atcgataggt gcaacgatgg cagagcacgg taatcggcgt cagtttttga
      181 actatggact attaggagcc atgggcgctg ccgcggcttc cagtttagag gaacgaatcc
      241 ttcttgccgc ccttcaggag ggtgccccgg aaaaggcggc ccagaccccg gtagatgacc
      301 gcccgatgcc ttgcggtcgt atccgcaatc tcaccattag tcggatgtta attggaggca
      361 atctcatcgg cggttgggcg catagccggg atttgcttta tgtctcccga ttattcaagg
      421 cctataacac tgaggaacgc atattccaga cgttggcctt gtgtgagtcc gtagggatca
      481 acacagtgca gctcgatccg gcttgcatca atgtctttca tcgctacttg aaagagcgag
      541 gcggtaagat gcaggccctg gtgtgcatcc accccgatgc agacgcggga aaagtccggt
      601 cgcaaatcga agatttgctc aacaaaggcg ccacgttttt gtacacgcat ggtgaggtga
      661 ccgaccggca caccatggct gggcggctcg atatcctggc caagaccatg gaactcgtca
      721 aggaggccgg tgtgcccgcc ggcatcggta gccactcgct ggaaacgccc atgcaatgcg
      781 aggcgcaaaa tgtcggggcc gagttctacg tgaagacctt ccatcccgac aactactggt
      841 cagctacgcc gcccgaaaaa cggcaagagt ggtgctggta cttgccgcgg ggacaggaac
      901 ctgggaccta ccatgacaac atatggtgtc tggatgcgga aaagacggca gagtttttca
      961 aaaccgttaa gaagccatgg tttgcgttca aggtcctggc ggcgggagcc atccatcccc
     1021 gcgttggctt ctcctatgct ctgcggaatg gtgccgattt cctgatcgta gggatgttcg
     1081 attttcaggt ggcggaagac gttacgatct tcaaagacgc ctactacaag ttaggtcaac
     1141 aacggcaacg cgaatggtac gcctgatgcc gtggcggcgc tcgcagccga atcgtcccgg
     1201 atggccgggt tgattgcctg gcttcccgca aagcggtaaa acaacaagag agaggatatc
     1261 ttgccgaccg tcggcaattc tcaattcctt acctcgatcg gtaagacggt ctgggtttgc
     1321 acctctacta gcgctagcac gccaggtgcc taacaatggc cttggttact ttgcgtatcg
     1381 tcgatggcgt tgaccgcggt cgtgtgtacg aggatattcc aacccccatc acggttggcc
     1441 gcgaggaagg gaacgtggtg cgcctcagcg acgaacgggt cagtcggttt cacctcaaga
     1501 ttcaggaaga tcgcaatcgc ctggttttga ccgatctggc aagcaccaac gggacgcggg
     1561 tcaacgggga accggtgcag gttgcccaaa tccgccccgg cgatattatc accatcggac
     1621 gaaccgtcat cctggtgggt tcgcgcgaac agatcgctca acgtttggca gctatccgca
     1681 agaagaagga agaattggga gagaccggtg atattgaaat cagtccggag gtcgtatcgc
     1741 cgtggttttc tccgtcgttg gagcgggaat tggactggag cgagcaatcc aacgtcctcc
     1801 gtccgatcca tgagcttgtc cccccggatc tgcccgagga tctgtcaccg gcccaactcg
     1861 cccaattgac cgagcttctc cagtattttc atatccggtt gcgacgcctt gtccaggcgg
     1921 ctagcatgga tcatgcctct gaaagcgtga ccctcgagca gcgacactgg cagggccttc
     1981 tcgatcttta tgatcgcgtg gcccattatt tgcggatgat cggcgaacca ccgacctaag
     2041 cttcttcgaa atgcgattct ggaatcacca tgttgaacga ctgcccggtg ggttgtcaac
     2101 ccgtgccctt tttggggacc gttgccaccc cggagggcgc tcttttaggg ggcgagagtc
     2161 gtgctacaat cgcctaaaca tgcctgaggc atgatgctca ggagcttttc aagggaggaa
     2221 atcagcatgg ccacgccgcc atttgttcac cttcattgtc atagtcatta cagtctgctc
     2281 gatggtgctg gaaccgttga tcgccttttg gatcgggcac gtgatctttg catgacggcc
     2341 ctggcgctga ccgaccatgg taacctcttt ggtgccctcg agttttatca aaaggccaaa
     2401 gatcgcggga tcaagccgat cgtcggatac gaagcctacg tggccccggg cagtcgcttt
     2461 cagcgcgaag ccgctagcat gaaggaggcc agttatcacc tcacactgct ggctcaggat
     2521 aaacgcggtt ttcaaaacct gctgcgactt gcttccgccg cttatctgga aggtttttat
     2581 tatcgcccac ggattgacaa agagcttttg gcacagcatc acgagggcct catctgtctg
     2641 agcggttgtc cttctggaga attgagtcga accctgcttg ccaaggaaga ccccgacatc
     2701 gcacgggcat gtgaggtggc ggattggttc cgcagcctct tcggtgaccg atatttcatc
     2761 gagatccaag ataatggcac ggaaatccag cggttggcga tgaaaggggc cattgccgtg
     2821 gccaagaaga tgaacatccc gctggtcgcc acaagcgacg ttcattacgt catgcccgag
     2881 gatgccgacg cccaagatgt cctactctgc atcaacaccg gcaaattccg cagcgacacc
     2941 aaccggatga agatggatgg gcgagagtac tacctgcgct cgccggagca gatgtaccaa
     3001 gttttttcgg gcttggaaga ggctgtgcga aggacgcaag acattgcgtc catggttgat
     3061 ttggaattgg aactggggcg ccgtcacttt ccaatttaca ccccaccaga cggcaagact
     3121 gccgagcaat acctgcggga attgtgtatt gagggcctca aagaacgcta ccgtcacgac
     3181 ccgcggcgat tccgcaatgg cgaactggcg ccggaggtca tggagcggct ggaaaaagaa
     3241 ctttacgtca ttaacactct aggctttgct aattattttc tgattgtttg ggatttcgtt
     3301 cgctttgccc gatcgaaggg gattcaggcc acggcccgcg ggtcgggtgt cggctcgctc
     3361 gtgagctacg cactcaaatt gagccacgtt tgccccattg aatacggcct tctctttgaa
     3421 cggtttctgg atctcaatcg ccgt
//
LOCUS       DAOPTR010000342         3444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_21859_length_3444_cov_3.572735, whole genome shotgun sequence.
ACCESSION   DAOPTR010000342 DAOPTR010000000
VERSION     DAOPTR010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21859_length_3444_cov_3.572735"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1136)
                     /locus_tag="PKI05_07555"
     CDS             complement(135..1136)
                     /locus_tag="PKI05_07555"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOA52097.1"
                     /translation="MNRWAGGFLVIVAVALVVLGVTPFAGGNWIVPWRATGPESLVAR
                     QILFELRIPRVLIGFVAGAALAVGGVAFQAMFRNPLATPYTLGVASGASLGAVISFQC
                     GLAFSTWGFSTTAVFAFLGAMATLLVVYAVAMWRAELSVATMLLAGVAMSFFNSSLIL
                     ILQYLGDPVQVFHAFRWMLGGLEGTIGYDDLKVLLPVVLLSSVVVFALRRELNLLALG
                     DEWATARGVPLMKIKALLLFVTGSMVASVVALCGPIGFVCLIAPHITRLLVGPEHGRL
                     IPAAALLGGAFLVVCDTVARTVLAPTEIPVGVITALLGGPFFLWLLFTRPLSWEWLS"
     gene            complement(1133..1927)
                     /locus_tag="PKI05_07560"
     CDS             complement(1133..1927)
                     /locus_tag="PKI05_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA52098.1"
                     /translation="MIPIVSIKNLSLRLSGQEILRDISFDLQAGDYVCLVGPNGSGKT
                     SVLRCVTRVYSRWLGEITVLGKPVRSFSQRELARHLAYLPQADGIAPPYTVEDFVLMA
                     RYPFVNPFRPPSAEDREAVEMALRMTELWSLRHRVVRTLSGGERQKVYLAGAIAQQAK
                     VLLLDEPTTFLDVPHQEDVRSLLHRINRQSGVTILAATHDLNAAVLSANRILALREGR
                     LLFSGSPEEFMTTEVVRQVFGFAPQFVRHPQVPLPMIVPSPGGGAT"
     gene            2148..2729
                     /locus_tag="PKI05_07565"
     CDS             2148..2729
                     /locus_tag="PKI05_07565"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOA52099.1"
                     /translation="MTNPADKVSFTELVAAAGRGDEEALTRLLEYCEPKFRIIARVSL
                     GPLLRPQLDTMDLVQSMKRMLIPGLRAGLYELDSPDQLVALAATIIRRKVALYWRRQK
                     KQAVLRLTEQALTGEEDALVEHRIQDWENEELLARLLESLTEEERQLFRMQLEGLTIV
                     EIARHLGCRPGPLRARLSRLRKKLRSLAGISWS"
     gene            complement(2798..3313)
                     /gene="rimI"
                     /locus_tag="PKI05_07570"
     CDS             complement(2798..3313)
                     /gene="rimI"
                     /locus_tag="PKI05_07570"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOA52100.1"
                     /translation="MSVKSQSGVRTLVRWMLREDLQAVLKIEHESFEFPWTESEFLAC
                     IQRPNCIAMVAVVQEKVVGYMIYEMSPQSFQILNVAVDAEYRRNGVGRALVQRLINKL
                     SPQRRHKISLEVRETNLPAQLFFKALGFQAKAILHDHYPDTAEDAYLMTYEIAPQVNP
                     GFRNRLAKFFR"
BASE COUNT          850 a         1038 c          907 g          649 t
ORIGIN      
        1 cccagcgtga ccggccttgg tacgaattgt aacggtttgt cggccaagtt ccagcgtcgc
       61 aacaggctac accaatgacg gggaaccacc cactgccctg gccgcatggg ctcgtagtcg
      121 cgaatgccga ggcgttacga aagccattcc caggaaagtg gccgcgtgaa aagcaaccaa
      181 aggaagaaag gtccaccgag cagggccgtg atgacaccga cgggaatctc cgtaggagca
      241 aggacggttc gcgccactgt gtcgcagact accaaaaatg caccccccag cagggccgcc
      301 gccggaatga gccgaccatg ctctggtccc accaaaagac gtgtgatgtg tggagcgatc
      361 aaacacacga acccaatcgg accgcacaac gccaccacgg acgcaaccat gctgcccgtc
      421 acaaacaaaa gcagggcctt gatcttcata agcggaacac cgcgagccgt tgcccactcg
      481 tcgcccagtg cgagcagatt cagctcccgc cgcaaagcga acacaacaac cgagctgagc
      541 aggacgacag gtaacagcac cttaagatcg tcgtagccga tggtgccttc caaacctccg
      601 agcatccagc gaaaggcatg gaacacctgc accggatcgc ccaggtattg cagaatgaga
      661 atcaaactgc tgttgaaaaa gctcatcgcc acaccggcca acagcatggt cgccaccgac
      721 agctcagccc gccacatggc cacggcataa acaaccagaa gcgtcgccat agcccccaag
      781 aaggcaaaaa cggcagtggt tgaaaaaccc caggttgaaa aggccaatcc gcactgaaaa
      841 ctgatcacag cccccagcga cgccccgctg gccactccca aggtgtatgg tgtggctaag
      901 ggattccgaa acatcgcctg aaaagccaca ccgcccacgg ccagagccgc accggccaca
      961 aatccgatca atacccgagg aatccgcaac tcaaagagga tttgtcgcgc caccaggctt
     1021 tccggtcccg ttgccctcca ggggacaatc caattgccgc cggcaaaagg tgtcacgccc
     1081 aacaccacta gggcaacagc cacgatgact aggaaaccgc ccgcccatcg gttcacgtgg
     1141 cacctccccc gggtgacggg acaatcatcg gcagtggcac ctggggatgg cggacaaatt
     1201 gcggcgcaaa tccaaagacc tgtcgcacca cctcggtggt catgaattct tcgggcgaac
     1261 cggaaaagag aagcctccct tcccgcaacg ccaaaatcct attggccgaa aggacggccg
     1321 cattcaagtc gtgtgttgcc gccaagattg tgacgcccga ctggcgatta atacggtgga
     1381 gcagggaacg cacatcctcc tgatgcggca catcgagaaa cgttgttggt tcgtccaaga
     1441 ggaggacctt tgcctgttgg gcaatggccc cggccagata caccttctgg cgttccccac
     1501 cgctcagcgt tctcaccaca cgatgccgca acgaccacag ttcggtcatc cgcagggcca
     1561 tctcgaccgc ctctcgatct tctgcactcg gtggccgaaa ggggttgacg aatggatacc
     1621 gggccatcag aacaaaatct tccaccgtat agggcggggc aattccatca gcttgaggta
     1681 aataagccaa atgacgcgcc aactcccttt gtgaaaaact ccgaactggt ttacccaaaa
     1741 ccgttatttc ccccagccat cgcgaataga cccgcgtcac acaccgcagc acactcgttt
     1801 tccccgaacc attggggcca acgaggcaaa cgtaatcccc cgcttgtaag tcaaaagaaa
     1861 tatcgcggag aatttcctgc cctgataggc gtaagctcag attttttatg gagacgatcg
     1921 gtatcacgat ggtaacggca ttcctgttgc tggggtaaaa ttgaccctgg cttttgccac
     1981 gggaacacgg gcagccgggt gaaatgggtg tcaccttcga ttatacttaa agcaaaagaa
     2041 gcgttgggtt gtgtcaggtg gttgccctgg cgacgcgctc tcgaagtacg tctttcgatg
     2101 cgcgtttaat agggcaatcg gcgccacgcg atgggaaggg ttcggttgtg accaatcctg
     2161 ctgacaaggt atccttcaca gagctcgtcg ctgctgccgg acgaggtgat gaagaggccc
     2221 taacccgcct gttggaatac tgtgagccca agttccgcat catagcccgg gtctcgctcg
     2281 gtcctttgct tcgtccgcaa ctcgatacca tggaccttgt tcaatcgatg aagcggatgc
     2341 tcattcctgg tttgcgagcc ggactttacg aattagattc ccctgaccaa ctggtggcct
     2401 tggcggccac gatcatccgg cgaaaggtgg ccctgtactg gcgacgtcaa aagaaacagg
     2461 ctgtgctccg cctcacagaa caagcactca cgggcgaaga agatgccctc gttgagcacc
     2521 gcatccaaga ctgggaaaat gaagagctcc tcgcacgact gctggaatcc ttaacggagg
     2581 aagaacgcca acttttccga atgcaactag aagggctgac aatcgtagaa attgcccgcc
     2641 acttgggttg tcgccctgga ccactccgcg cccgattgag ccgtttacgg aagaagctgc
     2701 ggagcctggc cggcatttct tggagttgat ccctggcgat ccctcatcgg ccgtgaaatc
     2761 gctactctcc gtttagcgcg cttccgaagt ttctcagtta tcgaaaaaac ttggccaaac
     2821 gattccggaa gccggggttg acctgggggg caatttcata ggtcatcaaa taggcgtcct
     2881 cggcggtgtc agggtaatga tcgtggagga tggccttggc ctgaaatccc agggccttga
     2941 aaaacaactg cgccgggaga ttagtttccc gaacctctag actgatctta tgtcgccgct
     3001 gcggcgaaag cttgttgatc aatcgctgca ccaaggcccg gccaacaccg ttccggcggt
     3061 attcggcatc gacggccaca ttgagaatct ggaacgattg tgggctcatc tcgtaaatca
     3121 tgtagcccac aaccttttcc tgaaccaccg cgaccatggc aatgcaattc ggtcgctgaa
     3181 tgcacgccaa gaattcagac tcagtccaag gaaactcgaa gctttcgtgc tcaatcttga
     3241 ggacagcctg gaggtcctcc ctgagcatcc agcggacgag ggttcgcacc ccgctctgag
     3301 atttaacact catggcaaac cttccttgtt agccagagtc aagatacctc ccagcagccg
     3361 acgcattatg gggcttaccc ctcctgccgt ttgagctgaa gtttggtcgt cgccttgccc
     3421 caaccatagg caatagatta gcac
//
LOCUS       DAOPTR010000343         3443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_21867_length_3443_cov_2.710153, whole genome shotgun sequence.
ACCESSION   DAOPTR010000343 DAOPTR010000000
VERSION     DAOPTR010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21867_length_3443_cov_2.710153"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="PKI05_07575"
     CDS             complement(<1..831)
                     /locus_tag="PKI05_07575"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOA52101.1"
                     /translation="MPLAAIVGLLVITGGWDVRGSNQSLRAEEKLGWIERADPRLDAL
                     IDSDAVVEKLAEGFDWSEGPVWVPRDNCLLFSDVPQNVVYRWREGQGIDVFLKPSGYT
                     GSVARGGESGSNGLALDREGRLLLCQHGDRRVARWENGRFVTLADRYQGRPLNSPNDL
                     VVKSNGDIYFTDPPYGMTAESRRDPNALPFCGVYRISRTGELTLLVSDMTRPNGIAFS
                     PDEKTLYVAQSDPERPLWMAFPLKEDGTLEEGKVFFDAKPWLEAGLKGLPDGMKVDVH
                     G"
     gene            complement(880..1086)
                     /locus_tag="PKI05_07580"
     CDS             complement(880..1086)
                     /locus_tag="PKI05_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52102.1"
                     /translation="MQDVGTQVALALIPLPGYFAQFPGSTISYYQPVAYASRKGYDFP
                     QNVELVLEHEGVFHVDRQYLVGLA"
     gene            complement(1262..2872)
                     /locus_tag="PKI05_07585"
     CDS             complement(1262..2872)
                     /locus_tag="PKI05_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52103.1"
                     /translation="MRNWSLPVLLTLLLFVRPHLALSDLPAIESVTTTPNRVIVVNGR
                     PFLPIMAWLQPPDNFAILKKCGINTTAGYWPGSGGTKDVADYMTRVREAGLYGVMPYH
                     PALHNHPNLLAYIHDDEPDLPRQVSDAKVTPGEGLRINARTPLWRMVDGDESSWSVLD
                     PLENATWTVTLNQPETVQRVAIWVTVSPGLSLPEEVVLSAEDKEVGRFSLEPKRGKQE
                     FALPSPVTAQEWRFTVTKVKPGEQVWGSISEIAAYTAEGKNVFLSPPRWVPRAMPEET
                     LKVYRAVKAADPSRRMFMTFTGYFHPHFGKWSDEQRQNLYPAYIQATDVVGFDIYPIY
                     GWNKPEWIHLVYEATEQLTALGGHRPVYAWIETSKGGQYTGDLERQKDVRPEHIRAEV
                     WMALCGGATAIGYFTHIWKPSYRQFGVPEENQVALRAINDQLTRLAPAILSKRNTREL
                     RLSISPEVRIAAFSTEYEEFMYVFAVNYDSGLKTAEVTFHIPSVDDSQSATVEVIDED
                     RSLPLRDGQFTDTFAPLAVHIYRLRRPR"
BASE COUNT          766 a          941 c          932 g          804 t
ORIGIN      
        1 tccgtggaca tcaaccttca tcccgtcggg cagtcctttc aatcctgctt ccagccacgg
       61 cttggcatcg aaaaagacct tcccttcttc caatgttcca tcttccttca acggaaatgc
      121 catccaaaga ggacgctccg gatcggattg ggccacgtaa agcgtcttct cgtcggggga
      181 aaacgcaatg ccattcggcc gggtcatgtc gctgacaagg agcgtcagtt cgcccgttcg
      241 actaatgcga tataccccac agaaaggtaa ggcgttggga tcgcgccgtg attccgccgt
      301 cattccgtaa ggtggatctg taaagtagat gtcgccattt gacttgacta ccaaatcatt
      361 ggggctattg agtggccgcc cttgatatcg gtcggcaagc gtgacaaagc ggccattttc
      421 ccaccgggca acccgacgat cgccgtgctg acacaagagg agtctgcctt cgcgatccag
      481 ggccagtccg ttggaccccg attcgccccc gcgtgcgacg ctcccggtat aacctgacgg
      541 tttgagaaaa acgtcgatgc cctgcccttc cctccagcga tatacaacgt tttgtggcac
      601 gtcggagaaa agcaaacagt tgtctcgagg aacccacaca gggccctctg accagtcgaa
      661 gccttctgcc aatttctcga ccacggcatc cgagtcaatc agggcatcca aacgcgggtc
      721 agcgcgttca atccaaccaa gtttctcttc agctcgcaaa ctttgattgc tacctcgaac
      781 gtcccatccg ccagtgatga caagaagccc gacgatcgct gctaggggca tagtccctcg
      841 gtttcgccat ggtattggta gcatcgcaca cctccggctc tacgcaagcc cgacaagata
      901 ctgacgatcg acgtgaaaca ctccttcgtg ctccaggaca agttcaacat tttgcggaaa
      961 atcataaccc ttccgacttg cgtaagcaac cggttggtag taggaaattg ttgaccccgg
     1021 aaactgggca aaatatccag gaagaggaat gagggccaag gctacttgcg tgccgacgtc
     1081 ttgcatcggc tacgggtatt cggtggacga tatttttgag cccacgcgtc ccaactccag
     1141 gcggggccgc aatcctattg ccgcaaaagg acgtggtctc attgactacc attttatgcg
     1201 gctgggaatt gcttccaaca aaatcccgat ttgcgtactc cacttccccg tccgctgtgg
     1261 cttatcttgg tctgcggagg cggtaaatat gcaccgccag aggggcaaag gtatcggtga
     1321 attgtccatc ccgtaggggc aagctacgat cctcgtcaat gacttccaca gttgctgatt
     1381 gcgagtcatc tacagaaggg atgtgaaacg tcacttccgc cgtcttgaga cctgaatcgt
     1441 aattgactgc gaacacatac atgaactctt catattccgt ggaaaaggcg gcgatgcgaa
     1501 cttcgggtga aatgcttagt cgaagttctc ttgtgtttcg cttgctcaga atagcgggtg
     1561 ccagacgggt cagttggtca ttgatggccc gaagtgccac ttggttttct tccgggacgc
     1621 caaattgccg ataggatggc ttccaaatat gggtgaaata gccgatggct gtcgcgccac
     1681 cgcaaagggc catccatact tccgcgcgaa tgtgttcggg tcgcacatct ttctgtcgtt
     1741 cgaggtcccc cgtgtattgc ccgcctttac tcgtctcaat ccaggcataa actgggcgat
     1801 gaccccccaa ggctgtgagt tgttctgttg cttcatagac caaatggatc cactctggct
     1861 tgttccatcc gtagatggga tagatgtcaa accccacgac gtccgtggcc tggatgtaag
     1921 caggatacag attctgtcgt tgttcgtcgc tccatttgcc aaaatgggga tgaaagtagc
     1981 cggtgaacgt catgaacata cgccgcgagg gatcagcagc cttgacggcg cgatagacct
     2041 tcagcgtctc ctcaggcatc gcccggggaa cccaacgtgg cgggctgagg aagacgtttt
     2101 tgccttcggc cgtgtaggcc gcaatctcac tgatcgaacc ccaaacctgc tcgccaggct
     2161 taactttggt caccgtaaaa cgccattcct gggcggttac cggggaagga agcgcaaatt
     2221 cctgcttacc ccgttttggc tcaagggaga atcgccctac ctctttgtcc tcggcggaaa
     2281 gaacaacttc ttcgggcaaa ctcaggcccg gtgaaaccgt gacccagatg gccacacgtt
     2341 gcaccgtctc tggctggttg agtgtgactg tccatgtggc gttctccagg ggatccaaga
     2401 ccgaccagga actttcgtcc ccatcaacca ttctccacaa aggtgtgcga gcgttgatcc
     2461 ggaggccttc ccccggggtg accttcgcat cgctcacctg tcgcgggaga tcgggctcgt
     2521 catcgtggat ataagctaaa agattcggat ggttgtgaag tgccggatga taaggcatca
     2581 cgccgtagag gccggcttct cgaactctcg tcatataatc cgcgacgtct tttgttccac
     2641 cggaccccgg ccagtaaccg gctgtcgtgt tgattccaca ttttttcaga attgcgaaat
     2701 tatccggcgg ctggagccag gccatgatgg gtagaaatgg gcgtccgttc acgacgatga
     2761 cgcgattcgg ggtggtggta acagactcaa tggcgggaag gtcgctcaag gccagatgag
     2821 gacgaacgaa caataacaac gttagcagaa ccggcagcga ccaatttctc atacggcatt
     2881 ctccttatgg aagtgcaatc cgactcattg attggcacat ctgatgtttg gggtaagctt
     2941 agcccacggc gaacctctcg tcacgatgtc aaagctctca taacttggat ctgagcatgt
     3001 aacgacgaga attgtaccct gcggcgtgcc gattcggaac acgaggacga caaccttggc
     3061 aagcctcggg gacaactacc aggacatctt tcaggccgag cttgccgctg gtggacgtga
     3121 ggattacatt gacgattttg ccaacgttcg tgtttgcccg ttattggtgg ttacatccgt
     3181 tgtatttgca cgacccaacg gagggttcga gatgggaata agacggtcac cggcaaatct
     3241 tatgattggg aacaagtggt tgtctggatt tgctgtgcac ggggttccgc gcctggtgtg
     3301 gcaaatgctc gtactgtttt cttggccgcc gcggtgttag gggggtacgg ttcttcacaa
     3361 gcctgggcgg cagagccggg agtcgatgcc acaacgactc tgaaggtgca attgtcaaca
     3421 atggatttga tcattgtgtt cgg
//
LOCUS       DAOPTR010000344         3439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_21894_length_3439_cov_3.628842, whole genome shotgun sequence.
ACCESSION   DAOPTR010000344 DAOPTR010000000
VERSION     DAOPTR010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21894_length_3439_cov_3.628842"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PKI05_07590"
     CDS             complement(<1..276)
                     /locus_tag="PKI05_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52104.1"
                     /translation="MNNKPNFASILDESPTEIDRPKPIPTGTYLCRVQGSPVYDKSSK
                     KGTPFVQFTLKPISAEDDVDEEDLAEMGGLDNKTLRLTFYLTEDAVYR"
     gene            complement(294..1130)
                     /locus_tag="PKI05_07595"
     CDS             complement(294..1130)
                     /locus_tag="PKI05_07595"
                     /inference="COORDINATES: protein motif:HMM:NF024870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOA52105.1"
                     /translation="MPSLAQHQSNQFTKVLLIGDAKSGKTGSLVSLVKAGYKLRILDF
                     DNLLDILKFKIMEECPDKIENVEFVTVRDAYKAGASGSQIDGKPKAWINAIKLLDNWK
                     YDDIDYGRPADWGPDTILIVDSLSRLCDAAYDFHESIIPRGKSGDYDGRAVYGNAQDD
                     VEKVLAMLTSRGFATNLIVIAHGSYIDLPDGSTKIFPQGIGQKLSPKIPQYFPTYIRY
                     KNKSGKRTIQTASDAMIDLANPRPDKVDKDLPIETGLATLFEALRDAPAEKPKSVTLV
                     RK"
     gene            complement(1130..1381)
                     /locus_tag="PKI05_07600"
     CDS             complement(1130..1381)
                     /locus_tag="PKI05_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52106.1"
                     /translation="MATQTKAQESIDTLKNVNKHLTEVFKRVDEKDAALNYVANDLRQ
                     LAAKCGELKVEAFVSDRYQNILLRARLNELATKLEGAKS"
     gene            complement(1597..2592)
                     /locus_tag="PKI05_07605"
     CDS             complement(1597..2592)
                     /locus_tag="PKI05_07605"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOA52107.1"
                     /translation="MPDQTLDESLLTTGSASPFLPGTNIQYAYDSTSLGLLKTCPRLY
                     QYTMIDGYVAKGESIHLRFGIEYHQALQDYDIARATGISHEDAVHDAISDLVRRTHDW
                     SVDETAKPGKYKNRTTVVSLVLDYLDHYVDDPAETYIKSDGRPAVELSFRFELDWGPS
                     YLGGTDSVTHPYLLAGHLDRVVTFNDQLFVMDHKTTVTTPSQYYFDQYEPHNQMTLYT
                     LAGQMVLGAPIKGVIVRAAQISLEKENRFVSGFTFRTADQLDEWLADLHLHLENAERY
                     AIANYWPMNDVSCNNYGGCKFRGVCSKSPSVREIYLKSDFIKLEPEDRWNPIKSR"
     gene            complement(2824..3048)
                     /locus_tag="PKI05_07610"
     CDS             complement(2824..3048)
                     /locus_tag="PKI05_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52108.1"
                     /translation="MREELIREILDKALEQELGMVVTCDNLHQTTLRFHAVTKNNPKY
                     KELMICAGSKPDQMLITKRTVELDDAREPE"
     gene            complement(3045..>3439)
                     /locus_tag="PKI05_07615"
     CDS             complement(3045..>3439)
                     /locus_tag="PKI05_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52109.1"
                     /translation="AKYPLRDRYPPEGTSNLYQEPLMALTDSIAAYDDCFQAFERAAK
                     SKKGIRILFDSKKTANYFRLRMNYARVLQRREAVRMYERTDPRFGKSEFDKFRLKIVE
                     AAEQTGEWWVYIDPFGMEREIMEVEELE"
BASE COUNT          734 a          784 c          954 g          967 t
ORIGIN      
        1 gcgatagaca gcatcctcgg tcaggtagaa cgtcaggcgc agggtcttgt tgtcgagccc
       61 acccatctcg gcgaggtctt cttcatcaac atcatcctcg gcagagattg gcttgagggt
      121 gaactgaaca aacggggtgc ctttcttgga agacttgtcg tagactggcg aaccctgaac
      181 gcggcagaga taggtgccgg tgggaatggg cttgggccgg tcgatttcgg tgggcgactc
      241 gtcgaggatg gatgcgaagt tgggcttgtt gttcatgggt tttcctttga ggtttattta
      301 cggaccagcg tgacagactt aggtttctct gcgggcgcgt cacgaagcgc ttcgaagaga
      361 gtcgccaaac cagtttcgat aggaaggtcc ttatcgactt tatctggtct gggattggca
      421 agatcgatca tggcatccga cgcggtttgg attgtgcgtt tgccagattt gtttttgtag
      481 cggatgtaag ttgggaagta ctggggaatc ttcggagaga gtttctggcc aataccctgc
      541 ggaaaaattt tggtgctgcc gtctggcagg tcaatgtagc tgccgtgggc gatgacgatt
      601 aggttggtag cgaagcctcg gctggtgagc attgcgagaa ctttttccac gtcgtcttgg
      661 gcattcccat aaacggcgcg tccatcgtaa tctccagact ttccacgagg aataatactt
      721 tcatgaaagt cataagcggc atcacacagt ctgctgagag agtccacgat aagtattgtg
      781 tcgggacccc agtctgcggg tcggccatag tcaatgtcat cgtatttcca gttgtccaga
      841 agcttaatag cattgatcca tgcttttggt tttccatcga tctggctccc actggctccg
      901 gctttatagg catcacggac ggtgacgaac tcgacgttct cgattttgtc gggacattct
      961 tccatgatct tgaacttaag aatgtcgagc aggttatcga agtcgaggat gcggagtttg
     1021 tatccggctt tgacgaggga tacgagcgag ccagttttgc cagacttggc atcaccgatc
     1081 aggaggactt tggtgaattg gttcgactgg tgttgagcta gggatggcat tagcttttgg
     1141 ctccttcaag cttggtggcg agttcgttca aacgggccct gagaagaatg ttttgataac
     1201 gatccgatac aaaggcctcc actttaagtt ccccgcactt agcagcaagc tgacgaagat
     1261 cgttggcaac ataattcaat gccgcatctt tttcatcaac cctcttaaac acttccgtca
     1321 ggtgcttgtt cacgttcttc aacgtgtcta tcgactcctg tgctttggtt tgcgttgcca
     1381 ttggctttct ccatttccgc gacaaattcg gtgtggattt cattaaggac ttcaacgatc
     1441 gctttgcgtt cggagaagcc tcgggtttgg tcgtctttta tgcggagtgc gaatttccaa
     1501 agaacctgtg aacagaggtc aaggccaacc ataaagcctg ctctggcact ggcgcttggg
     1561 aattctatcg gcttctcagg ggattcagtc atgacatcac ctcgatttaa tgggattcca
     1621 gcgatcttcc ggctcaagtt ttatgaaatc tgacttgaga taaatctcgc gaacacttgg
     1681 tgattttgaa cacacgcctc taaatttaca accaccgtag ttattacaac ttacgtcgtt
     1741 cattggccaa tagttggcga tggcatagcg ttcggcattc tccaagtgca ggtgaagatc
     1801 agccagccac tcgtcaagct ggtccgcggt tcgaaaggtg aaacctgaaa caaagcgatt
     1861 ttccttctcc aaggaaatct gcgcggcacg gacgatcaca cctttgatcg gggcaccgag
     1921 gaccatctgc ccggcgaggg tatagagggt catctggttg tggggttcgt attggtcgaa
     1981 gtagtattgg cttggggttg tgacggtggt tttgtggtcc atcacgaaaa gttggtcgtt
     2041 gaaggtgacg acacgatcta ggtggccggc taggaggtag gggtgcgtta cagaatctgt
     2101 accacctaga tagctcggcc cccaatcaag ctcgaaccga aagcttaatt ccactgcggg
     2161 ccttccgtcg gatttgatgt aggtctcggc aggatcgtcc acgtaatggt caaggtaatc
     2221 cagaacaagt gaaacgacag tcgtgcggtt tttatacttt cccggtttag ctgtctcatc
     2281 gacactccaa tcatgcgtcc ttcgaaccaa gtcggatatt gcatcgtgaa ctgcgtcttc
     2341 atgggagata cctgtggcgc gggcgatatc gtagtcttgc agagcttggt ggtactcgat
     2401 gccaaatcgc aggtggatgg attcgccttt ggcgacataa ccatcgatca ttgtatactg
     2461 atacaatcgc gggcaagttt taagtagtcc aaggcttgta ctatcataag catattgtat
     2521 gttggttccg gggagaaagg gactggcgga gccagtggtt aggagggatt cgtctagggt
     2581 ttggtcgggc atggctttag aatctcctat caaccttaac ctcagtcttt ggcttagtca
     2641 gtttcatcat cacttgggta atgtcgactg actggggttt ggctggtttc tcgcctttgg
     2701 ccttacgggc tcgagagtgt cgatgatatt tgattatgtc atcgatatct tcggaagtca
     2761 agtcgatcgc aggtttggcg tttatgtctt cgatccggcg catcaattca tcaagatcac
     2821 tcatcattca ggctcccttg catcatcaag ttcgacggta cgtttggtga taagcatttg
     2881 atcaggtttt gacccggcac agatcattag ctctttgtac ttggggttgt tcttggttac
     2941 ggcatggaat cgtaaggttg tctgatgtag gttgtcgcag gttacgacca tccccagttc
     3001 ctgttccaag gccttgtcga ggatttcgcg gatgagttct tctctcattc gagttcttcg
     3061 acctccatga tttcccgctc catgccgaat gggtcaatgt agacccacca ttcaccggtt
     3121 tgctccgcgg cttcgacaat cttgaggcgg aacttgtcga actcggattt accaaagcgc
     3181 ggatcggtgc gttcgtacat gcggacggct tcacggcgtt gaaggacacg agcgtagttc
     3241 atccgcaggc gaaagtagtt ggcggtcttt ttgctatcga agaggatgcg gatgcctttt
     3301 ttggatttgg ccgcgcgctc gaatgcttgg aagcaatcgt cgtaggcggc gatggagtca
     3361 gtcaacgcca tcagtggctc ctgatatagg ttagacgttc cttcgggcgg gtatcgatca
     3421 cgtagtggat attttgctc
//
LOCUS       DAOPTR010000345         3438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_21903_length_3438_cov_3.137748, whole genome shotgun sequence.
ACCESSION   DAOPTR010000345 DAOPTR010000000
VERSION     DAOPTR010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21903_length_3438_cov_3.137748"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..1330
                     /locus_tag="PKI05_07620"
     CDS             173..1330
                     /locus_tag="PKI05_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52110.1"
                     /translation="MITAIAILVMVGGGVPSAIAWSGTTYYEAEIIGKVMSPANSDQP
                     AVVYGDLKVKNSKWKWQTLSTGKFYKVSPKSTRTRQALNNQVGKGVRILGSYNSSFLQ
                     RGGLLTATYVEAKEIKEGPPQLTSFGVFRNPGQAVGIEIKHPVDFVARLAVLRDTMIG
                     MNGELSAPFLSMSPEQVQKFVADVDKDPKKKGIKTALNNEWRANWKTQNFVERDLDAY
                     NAALATARSVSFATGWAGTGLDALLDSVPGKRKDIEKAMLGNVKGGPAPDQEGAAVML
                     ARISSLDIDGFKLYWVEGPLFDTSQGLAQGIVSANERDGQTWRAQGMNPKRAEALDGQ
                     VCWVSGSRVIVRDPKTGAIVDKYFAVDKTMTYQETVSAFNLIEQLAKQGGI"
     gene            1330..2175
                     /locus_tag="PKI05_07625"
     CDS             1330..2175
                     /locus_tag="PKI05_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HOA52111.1"
                     /translation="MDKKKIAFRWIAASVFWFALIGCFFFSWYSMRLVADATSYAKGR
                     AITPEMVAEERVARETARGFVVDWGTFSVKNPNDYQTRIGAWLNKTIPVPQGEQKTTS
                     AAVIGIDRIEQGLWRTSLVIQGERWSQSKGAFATDAPVFAWNPFIQRVEVTVRVDKGK
                     GEIVGLPVIVPLDPGGWVEPEKLIEKESPSEDFVGFAKQALDLYFRGDDLSNFTLPNT
                     NIRSLGEWEMKDCKITAYEPTERGARAVIQAVVTTPGIDNLRQTIIVEAEKKDRWLLS
                     RLGGI"
     gene            2563..>3438
                     /locus_tag="PKI05_07630"
     CDS             2563..>3438
                     /locus_tag="PKI05_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52112.1"
                     /translation="MRQFPIKAFVDNIIFDQNRGIHAFYRLPSLPYRFFDQQRKQMVV
                     GILEEMLSGFAGHGQILLLWEEMNVDEGEYLNSCLRGAKQNEESIRHARSARAMLSQG
                     ARRLRRYLVLELKRSQGIANFKQLIEQFREIASTVGTTAPALSVPSETIAAAREIEEG
                     LAAKLKRYGIFRGDFLDLDFIIRRAVDRAGVLPIPLPERSAGRMTPGTIAAFTNGAKV
                     QEKLDFLEIKDTKKTHTQSFLHFVDYPRQMPSGGRAVFNPDSLGFPFDVVMHFEVLPP
                     HAAARKVDTKRRLLRA"
BASE COUNT          871 a          781 c          978 g          808 t
ORIGIN      
        1 acgatgatac tcagatccca ggtaatcccg gccattattg cgatgttggc gggggccttt
       61 ttgatgactg ttttggaccc cgatgtcatg aaagctattg gggaagggat taaagggttg
      121 ctcgaagggt cgaaaggagc gtgatccagt gagaaccaaa cgactaagat gcttgattac
      181 ggcaattgcc attctggtga tggtgggggg cggtgttccc tccgctattg cttggtcggg
      241 gactacatac tacgaggcag agataattgg taaagtcatg tcccccgcaa acagcgatca
      301 accagccgtc gtttacgggg acttaaaggt taaaaactct aaatggaaat ggcaaacgct
      361 ttcgacgggc aagttttaca aggtcagccc aaaatcaacg cgcacccggc aagcccttaa
      421 taatcaggtt ggaaaaggag tgcgaatcct gggttcgtat aactcgtcgt ttctccaacg
      481 aggcgggcta ttgaccgcga cttatgttga ggctaaagaa ataaaggaag gaccgcctca
      541 acttacgtcg ttcggggtgt ttcgtaaccc tggacaagcc gtgggaatag aaatcaagca
      601 cccggtagat tttgtggcga ggttagccgt gttaagagac acaatgatag ggatgaatgg
      661 ggaactctcc gcgccgtttc tatccatgtc gcccgagcaa gtgcaaaaat tcgttgctga
      721 tgtggataaa gaccctaaga agaaaggtat caaaaccgcg ctgaacaacg aatggagagc
      781 aaactggaaa acccagaatt tcgtagagcg cgaccttgat gcttataacg cggcgttagc
      841 cacggcgcgg tcggtttcat tcgcgaccgg atgggcggga accggattgg atgccttgct
      901 cgattctgtt cctggcaaac gcaaagacat agaaaaagca atgttgggta atgtaaaggg
      961 cggtcccgcg cctgatcaag agggtgccgc cgttatgctg gcgagaatct caagccttga
     1021 tatagacgga ttcaagctgt attgggtgga aggacctctt ttcgacacct cccaggggtt
     1081 agcccaaggc attgtgtctg ctaacgaaag ggacgggcaa acctggagag cgcaagggat
     1141 gaacccaaaa cgtgccgagg ctttagacgg ccaggtgtgt tgggtttccg gctcgcgggt
     1201 gattgtgaga gacccgaaaa ctggggcaat agtggacaag tattttgcgg tcgacaagac
     1261 tatgacctac caagaaacgg tttcagcatt caaccttatt gaacaactag ccaaacaagg
     1321 aggcatttga tggacaagaa gaaaatcgcg tttaggtgga tcgctgcctc ggtgttttgg
     1381 ttcgcattga tcggttgttt ctttttctcg tggtactcaa tgcggttagt ggccgatgcg
     1441 acatcatatg ctaaaggtcg cgccattacc cccgaaatgg ttgccgagga aagggttgcg
     1501 agggaaactg cacgggggtt cgtggtcgat tggggcacgt tttccgtcaa aaaccctaac
     1561 gattatcaga ccagaatcgg ggcctggctg aataaaacaa ttcccgtgcc tcaaggagaa
     1621 cagaaaacga cctccgccgc cgttatcggc atcgaccgca ttgaacaagg gctatggcgg
     1681 acatctctgg tgattcaggg cgaacgatgg tcgcagagta agggggcctt tgcgaccgac
     1741 gctccggttt ttgcatggaa cccgttcatc caacgggttg aggtcacggt gcgggtagat
     1801 aaagggaaag gagaaatcgt agggctgccc gttattgttc cattagaccc agggggatgg
     1861 gtcgaacccg aaaaattaat tgaaaaggaa tctccgtctg aggattttgt tggtttcgcc
     1921 aagcaagccc tcgatttgta ttttcgagga gatgatttga gtaatttcac gttaccgaat
     1981 accaacattc ggtcgctagg cgagtgggaa atgaaagatt gcaaaatcac cgcttatgaa
     2041 ccgaccgaac ggggtgcgcg agccgtaata caggccgtcg taacaacacc aggaattgat
     2101 aacttgcggc aaacgatcat tgttgaggct gagaaaaagg atcgttggct gttatcgcgt
     2161 ttagggggga tttgacatcg cctattcaag ctacaaaaac ctgttcagaa tcaagtggaa
     2221 aatccatgcg ataggcgaca agccgttgcc tcgcccgatc cctttggacg ggcttttcgt
     2281 tttcttgttg gttttattcc ctagcatgtt catcgggaaa ccgttagcgg ctgttgctga
     2341 gactaacgag gtattggccg gggtgctagt cgctttcctt gttacttggg catttttgaa
     2401 aatcgatcca caaggcaggt cgttgccgag ttttctcgga tcgctcatcc tatatgcgat
     2461 acgccctaaa acaatggttt tctcgggaga aattctcact cgacaaaagg aacaaagagt
     2521 ccattggtca tcgttacatt tttcagggga aggagcggaa caatgcggca attcccgata
     2581 aaagcgtttg tagacaatat cattttcgac caaaacagag gaatccatgc cttctatcgc
     2641 ttaccgtctc tgccgtaccg ttttttcgac cagcaacgga aacagatggt cgtaggcatc
     2701 ctggaggaga tgttgtccgg gttcgccggg cacggtcaaa tactgttgct gtgggaagaa
     2761 atgaacgtcg atgagggcga atacttgaat tcgtgcttgc gtggtgcgaa acaaaacgag
     2821 gaaagcatcc ggcacgcccg gtctgctcgc gccatgttat cccagggcgc acggcggtta
     2881 cgccgttact tggtcctcga actaaaacga tcccaaggga ttgcgaattt caaacaactc
     2941 attgagcaat tccgggaaat agctagcacg gttggcacta cggccccggc tctctctgtg
     3001 ccgtcggaaa caatcgcggc ggctcgtgag attgaagagg gcttggcggc gaaattgaaa
     3061 cggtacggga ttttccgggg ggatttcctc gacctcgatt tcatcatccg ccgggcggtt
     3121 gatagggcgg gggtcttgcc gataccgttg cctgagcgct cggcgggtcg tatgaccccc
     3181 ggaaccattg cggcgttcac caacggggct aaggtgcagg aaaaacttga tttcttggag
     3241 atcaaagata caaagaaaac ccatacacaa tcgttcctcc atttcgtgga ttatccgcgg
     3301 cagatgccaa gtggtgggcg cgctgtcttt aatcccgatt cgttggggtt cccatttgat
     3361 gttgtaatgc atttcgaggt gttgccgcct cacgcagcgg cgaggaaagt ggacaccaaa
     3421 cgtcgattac tacgagcg
//
LOCUS       DAOPTR010000346         3434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_21938_length_3434_cov_3.141758, whole genome shotgun sequence.
ACCESSION   DAOPTR010000346 DAOPTR010000000
VERSION     DAOPTR010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21938_length_3434_cov_3.141758"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            345..1346
                     /locus_tag="PKI05_07635"
     CDS             345..1346
                     /locus_tag="PKI05_07635"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOA52113.1"
                     /translation="MHEFGERVFHVLPRQVNMTVAAALREWLPGVSWSRIKKLIEGRY
                     IQVSGNLCLDPARRLKLQDVVKLLPRPGQAPPSPRDVAVVYLDEHIVVVDKPAGMTTN
                     RHREELDASARRRQLQPTLDEVLPHIIARMEGKKKRHKGVPPPVRAVHRLDRETSGLI
                     VFARTSEAERGLMEQFRKHTTTRRYLAIIPGYMEAQTIQSYLVRDRGDGRRGSTKKPG
                     EGRLAITHVKPVERIGDYTLVECRLETGRTHQIRIHLAEAGHPVCGEKLYNRPLFGKP
                     IEDNSGAPRLCLAAVELGFIHPVTGEPMNFEIPLPRDLMDFVYELRRRARLCQKKES"
     gene            1414..2328
                     /locus_tag="PKI05_07640"
     CDS             1414..2328
                     /locus_tag="PKI05_07640"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOA52114.1"
                     /translation="MPILGAHQSIAGGFEKAVERAVATGCECLQIFTKSCTRWKSPPL
                     SEEQIAAFQRALQRSGLRHPVGHDGYLINLASPDPKLWRRSLESLEDEYRRATQLGLE
                     ALIIHPGASIHNDTDAALRRIATALREVLDRNPESKTWILLETLAGQGSQVGRSFDEL
                     ATIFELMDDTQRLGVCWDTCHMLAADYPIDLPAGWDRTVRWFERQIGLDRLKAIHLND
                     SKTARGSGVDRHEHIGLGYVGRETFRRILQDPRVNHLPMYIETPKGKASDGRDWDVIN
                     LTLLRELAAERQDLLGSSPSDDRPRSLL"
     gene            complement(2552..2782)
                     /locus_tag="PKI05_07645"
     CDS             complement(2552..2782)
                     /locus_tag="PKI05_07645"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOA52115.1"
                     /translation="MPPVRAVRKAVRRSQCSNNLKQTKLGLHIFHDTYRRSPAVCYDP
                     QFQEYGGQRYRWFYLPDVENVLYLTHIRCPLR"
     gene            complement(2954..>3434)
                     /locus_tag="PKI05_07650"
     CDS             complement(2954..>3434)
                     /locus_tag="PKI05_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52116.1"
                     /translation="TMRIAAVLTLVAVTIAIAGCGQKKGVSFKTVPVTIKLTYKGQPL
                     DGANVQMYPLDPNGRGATGKTDANGAAVMRTFGDAGAMAEGAIPGKYKIIVTKVDVPQ
                     VSENINYEEYSKQQAEQNPYASAPKPLLPTKYSSPQTTPFECEVKDGPNEFSFELTD"
BASE COUNT          733 a          990 c          959 g          752 t
ORIGIN      
        1 ccctgggtct aagagcctat cccaaagttc cctctccctc tgggagaggg ttagggtgag
       61 ggcaagcgcc cgccggagtc catcgagaaa accgcagcga ttcgagccac cctccccccg
      121 actcttctcc cgccaaacgg gtaaggggag cattatggga taagctctta atggaaatgc
      181 cgccccggga tgtcaatttt tcgccaaatt gttatcctta gcattagatt tattgcgtag
      241 aattctttcc cagagactcc acatcacagg ttttctccaa tcgggcattg gcctgcctaa
      301 agacgttgca ccttgtctga actagttgag atcattgaat catcatgcac gaattcggtg
      361 aacgagtctt tcatgttttg ccgcggcaag tcaacatgac ggtggctgcc gcgctgcggg
      421 aatggttgcc gggtgtttcc tggtcgcgga tcaagaaact catcgagggc cgatacatcc
      481 aggtaagtgg caatttgtgc cttgatccgg caaggcggct gaagcttcag gacgtggtca
      541 agctcctgcc gcgcccagga caggcgcccc ccagcccgcg ggatgtcgcc gtggtttacc
      601 tcgatgaaca cattgtggtg gtggataagc cggcggggat gaccaccaat cgacatcgcg
      661 aggagctcga cgcctcagct cgacggcgac aactccagcc caccctcgac gaggtcttgc
      721 cgcatatcat cgcgcggatg gaaggcaaaa agaagcgaca caaaggcgtt ccaccaccgg
      781 tgcgtgccgt gcaccgcttg gaccgtgaaa ccagtggctt aattgtcttt gcgcgcacca
      841 gcgaggcaga acgcggtctc atggagcaat tccgcaaaca taccaccacc cgccgttatt
      901 tggcgatcat ccccggttac atggaagccc agacgattca gtcgtacctt gtccgtgatc
      961 gcggtgacgg acgaaggggc agcaccaaaa agccgggcga gggccgtttg gcaatcaccc
     1021 acgtcaagcc cgtcgagcga atcggcgatt acaccctggt ggaatgccgt cttgaaaccg
     1081 ggcggaccca ccaaatccgc attcatctgg cagaggccgg ccaccccgtt tgtggtgaaa
     1141 agctttacaa tcggcccctt tttggcaagc cgatcgagga caacagtggc gcaccgcgtc
     1201 tgtgtcttgc cgccgtagag ttgggattca ttcatcccgt aacaggagaa cccatgaact
     1261 tcgagatacc tttgccccgg gacttgatgg attttgtgta cgaacttcgg cgtcgggcaa
     1321 gactgtgcca gaaaaaggaa tcgtaacgat catgggtctg aggggggttg ggttggcccc
     1381 ccttctagtt gcctcgccat cttgagttga gccatgccga ttcttggtgc tcaccaatcg
     1441 attgccggcg gcttcgaaaa agcggtggag cgcgccgtcg cgaccggttg cgagtgcctg
     1501 caaatcttca cgaaaagttg tacccgatgg aaatcgccac ccctttcgga agaacaaata
     1561 gctgcctttc aacgcgcact acaacgcagt ggcttgcggc atcctgtagg acacgacggc
     1621 tatttgatca atttggcaag tcccgacccc aagctgtggc gcaggtcgct ggaatcgctc
     1681 gaggacgaat accggcgggc aacgcaattg ggcttagagg cccttatcat ccatcccggt
     1741 gcgtccattc ataatgacac tgatgctgcc ctccgccgca tcgccactgc cctgcgggaa
     1801 gttctcgaca ggaacccgga gtcgaagacg tggatacttc tggaaaccct ggcaggtcag
     1861 gggagccaag tgggccgttc ttttgatgaa ttggccacca tctttgagct gatggatgac
     1921 acccagcgac taggagtgtg ctgggacacg tgccacatgt tggcggcaga ttatccgatc
     1981 gatttgcccg caggttggga ccgtacggtc cgttggttcg agcgacagat cggactggat
     2041 cgcctcaagg ccatccatct caacgacagc aaaacagccc gtggcagtgg tgttgatcgc
     2101 cacgagcaca tcggcttggg ctatgtgggc cgcgagacct tccggcggat tttgcaggac
     2161 ccccgggtga atcatctgcc catgtatatc gagaccccaa aagggaaagc ctctgatggc
     2221 cgagattggg atgttatcaa tttgacactc ctgcgtgaac tggccgccga gcgtcaggat
     2281 ctattgggct catcacctag cgacgatcga ccccgctcgc tgctgtgacg cccaacaagc
     2341 ccgccgagct gcccccgtct ttgacccaat gcgtgatctg atctttggag acgcaatcgg
     2401 caagatcaaa caccgcggca gtctagctgc tggccacagc aaggccgagt tccaggttga
     2461 ccgcggatcc tgccaaattc cgacgcacgg atcaatcggt cctgcggcac caaggtcacg
     2521 tttgcccctt tgccgggctg cccgtgatac gttatcttaa tggacaccgg atatgtgtta
     2581 ggtacagcac gttttccaca tccggcagat aaaaccatcg ataccgttgc ccaccgtact
     2641 cctgaaattg ggggtcgtaa cacactgcgg gcgaacgcct gtatgtatcg tggaaaatgt
     2701 gcaatcccaa tttagtctgt tttaaattgt tcgaacattg cgagcgcctt accgctttcc
     2761 gtaccgctcg gaccggcggc aacaatatgg tgatgaggat gccgaaaagc gcgatacgat
     2821 aacccgaccg cgctggcctc tagagcctcc gctcagtgtc ccccaggtgc gcacctgccg
     2881 cgcaagccaa tcgcaggaag gcctttcaaa ccgaggtccc acccgtttgg cctgtgtcat
     2941 ccaaacgtgg cgatcaatct gtcagttcaa aactaaactc gtttgggccg tctttgactt
     3001 cacactcgaa gggtgtggtc tggggtgacg aatacttggt cggcaacagc ggcttggggg
     3061 ccgaggcgta gggattctgc tcggcctgtt gcttgctgta ttcttcgtaa ttgatgttct
     3121 cacttacttg ggggacatcc actttggtca cgataatctt gtatttgcca gggatggctc
     3181 cttccgccat ggctccagca tcgccaaagg tcctcatcac cgctgcaccg ttggcatctg
     3241 ttttacccgt ggcaccgcgc ccgttgggat ccaatggata catctggacg ttggcgccgt
     3301 cgagcggctg gcccttgtag gtcagcttaa tggtcaccgg tacggtcttg aaggagacgc
     3361 cctttttctg cccgcaccct gcgatggcaa tggttacggc caccaaggtc aaaacagcag
     3421 cgattctcat ggtt
//
LOCUS       DAOPTR010000347         3430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_21969_length_3430_cov_3.857778, whole genome shotgun sequence.
ACCESSION   DAOPTR010000347 DAOPTR010000000
VERSION     DAOPTR010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21969_length_3430_cov_3.857778"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..677
                     /gene="flgC"
                     /locus_tag="PKI05_07655"
     CDS             255..677
                     /gene="flgC"
                     /locus_tag="PKI05_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOA52117.1"
                     /translation="MFSALDISTSALVAQRTRMTAIAGNIANISTTHNELGQPEPYKP
                     RYVVFQADTSIGQYGASGVKVAAVRVESDRPPRYKYEPGHPDAIQSGPMKGYVAYPDI
                     NMMTEMTDALEAARAYEANLGTIEITKDLTQQTLRILA"
     gene            677..973
                     /gene="fliE"
                     /locus_tag="PKI05_07660"
     CDS             677..973
                     /gene="fliE"
                     /locus_tag="PKI05_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOA52118.1"
                     /translation="MNLIRPISPSSVTPVGPTGPGRVTQSGEFKDFLLRSINEVNSMQ
                     QEADRAVERLITGEDVTPAEVLTAVQKADIAFKLMMQIRNKLVQAYQELQNIRV"
     gene            1058..2698
                     /locus_tag="PKI05_07665"
     CDS             1058..2698
                     /locus_tag="PKI05_07665"
                     /inference="COORDINATES: protein motif:HMM:NF013664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52119.1"
                     /translation="MDFINKTWDQLKDVFLSLTPGTRVTTVLLLVVVLVSLGYLFTYS
                     GSGGSTYLMNGAHFSPTDLPAMEAAFAAKGLNDYRIEGTRIRVPLGKQDKYVAALAEG
                     NALPYNYLDILEKTINESSAFESRYKLEERTKIALQKELSRVISQMAGVQNAVVMFSR
                     LNQGGLRRETITTASVAIKPIGGQELSEQVVRSIRYYVAKSIGGIKPEEVVVTNMDTG
                     ANYYVTNDEPLQAAEGTYALKRSWETYFAEKVKTALGYVPGVLVSCNVELDPTKYQRQ
                     EQVKIDPKAVPLDVTETTKTRQSTSDPGGRVGYVAQGNTPRSLSGGNTPTPSETEEET
                     QRTEKNTVGQTVIQQETAGLLPKRVTVSVAIPASYFRRIWEQSQPTQANNPQGPTPQE
                     LDKIRTEEMTKIRNHVMALLPVVENVQDPSQLVTVTDFVDMPLPPPQPVSIVEQTFDF
                     LRVYWPTLGLMGLVVFSLIILRSAVRATPSAPAGVAPAVAAAAEETAAQEEQKKSEKK
                     KWRTSETGKSLRDEVAELVADDPETAANILKTWIGNPI"
     gene            2721..>3430
                     /locus_tag="PKI05_07670"
     CDS             2721..>3430
                     /locus_tag="PKI05_07670"
                     /inference="COORDINATES: protein motif:HMM:NF026191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52120.1"
                     /translation="MAHPGLRKAAIFIDCLDAKTADLVLDRLPREYADQVRSTWMALG
                     RIPAEEREQVIREFMQTTAQPSRETTLTSTPPQWEGTELILQSSMNAPAKKNEDTEGS
                     PPFRLLREAEADKLVGILQGERPQTIALVLAHLPPHQAGAVLVRFPPAMQVDIIRCLI
                     SLEETDPEVLREVDRALESKLSQLVAIQRRRIAGITAVNGIVHQSQEHVSQQILDNLA
                     NFAPDLAERLSPIPKIDF"
BASE COUNT          885 a          920 c          946 g          679 t
ORIGIN      
        1 ggcctatcct tcggcacgac gggaatgaca tttcgctgga acaacaagtg agtgagatgg
       61 tcaagaacca gattttgcac aatacggcga tcacgattat gaccaaccag tttcgtttac
      121 tcttgacggc aatcagtgaa catgtgtaat gattcgaccc catatcgttt tagaggtggt
      181 catcgcctag cctggcgtac gctcaggcaa ggtgagtacg ccattggctt gaatatctac
      241 tgatgaggtg agatatgttt tcggcattgg acatcagtac gagcgcgctg gttgcgcagc
      301 ggacacgcat gaccgccatt gccgggaata ttgccaacat cagcaccaca cacaatgagc
      361 tgggccaacc ggaaccgtac aaaccaaggt acgtggtctt tcaagccgac accagcatcg
      421 gacaatatgg ggccagcggg gtcaaggtgg cagcagtgcg cgttgaaagt gatcgcccac
      481 cgcgctacaa gtatgagccg ggacacccgg atgcgatcca gtcagggccg atgaagggct
      541 acgtcgccta tccagatatc aacatgatga cggagatgac agacgccctt gaggctgccc
      601 gagcctacga agccaatctt ggcaccattg agatcactaa agacctgacg cagcagacgt
      661 tgaggattct ggcctgatga acctgattcg tcccatcagt ccaagtagtg tcactcctgt
      721 tgggccgact ggacccggtc gggtaactca gtcgggtgag ttcaaggact tcctcctccg
      781 ctcgatcaat gaggtcaact ccatgcaaca ggaggccgac cgggcagtgg agcgtctcat
      841 cacgggggag gatgtcacgc ctgcggaggt cttaactgcc gtgcagaagg ccgacatcgc
      901 cttcaagcta atgatgcaga ttcgaaataa actggtgcag gcttatcagg aactccagaa
      961 catccgggtc tgaaattcgt ggcaaagagg aatgagagtg ccattagtga agtgatacca
     1021 ctcggttaac gacgattagt gatatggatg tccaaggatg gacttcatca ataagacctg
     1081 ggatcaactg aaagatgtct tcctctccct gacgccggga acaagggtga cgaccgtcct
     1141 acttctcgtc gttgtccttg tgagtctcgg gtatcttttc acctacagcg gaagcggtgg
     1201 atcgacctat ctcatgaacg gcgcccactt ctcgccaacc gatctcccag ctatggaggc
     1261 tgcgtttgcc gccaagggtc taaacgacta tcgaatcgag ggcaccagaa tccgcgttcc
     1321 cctgggaaag caagataaat acgtggcagc cttggctgaa ggaaatgccc tgccttataa
     1381 ctacctggat attctggaaa agacaatcaa tgagtccagt gccttcgaaa gccgctataa
     1441 gctggaggag cggacgaaga tcgccctgca aaaggaactc tcgcgggtta tcagccaaat
     1501 ggcaggcgtg cagaatgccg ttgtaatgtt ctcacgactt aaccaaggcg gtttacggcg
     1561 agaaaccatc accacggcga gtgtcgccat taagccgatc ggggggcagg agctttcgga
     1621 acaagtcgtg cgatccattc gttattacgt cgccaagagt atcggtggca tcaaacccga
     1681 agaggttgtc gtcaccaata tggacacagg ggccaactac tatgtgacca acgacgaacc
     1741 tctccaagct gctgagggga cctacgcttt gaaacgttcg tgggagacct actttgcgga
     1801 aaaggtcaaa accgccttgg gatacgtccc cggagtcctg gtaagttgta atgtcgaact
     1861 tgatcccaca aagtaccaac gccaggagca ggtcaaaatc gacccaaagg ccgttccgtt
     1921 ggacgtgacg gaaactacaa agacccggca atccacaagc gacccaggag gacgtgtggg
     1981 ctacgtcgcc caaggaaaca caccacgatc tctcagtgga ggcaataccc caacaccgag
     2041 cgaaacagaa gaagaaaccc aacggacgga aaagaacacc gttggacaga cggtgattca
     2101 gcaggagacg gcgggccttc ttcccaagcg ggtgaccgtt tctgttgcca tcccggccag
     2161 ttacttccgc cgtatctggg agcaatcaca gccgacacaa gccaacaacc cacaggggcc
     2221 gacgccacag gagctcgaca aaatacgaac ggaagagatg accaaaatca gaaaccacgt
     2281 catggccctg ttgcccgtcg tcgaaaacgt acaggacccc tctcaattgg tcactgtcac
     2341 tgattttgtc gatatgccat taccgccgcc acagccagtc agcattgtgg agcaaacgtt
     2401 tgactttctc cgggtttact ggccaacgct gggtctgatg ggcctggtgg tctttagttt
     2461 gattatcttg cggtcggcgg tgcgcgcaac cccctcggct ccggcagggg tggctcccgc
     2521 ggtagccgca gcggcagagg aaacagcagc tcaagaggaa caaaagaagt cagaaaagaa
     2581 gaagtggcga acatcggaga cgggaaaatc actccgcgac gaagtggcag agctggtggc
     2641 cgatgatccc gaaacggctg ccaacattct caagacgtgg atcggcaatc ccatttaatc
     2701 agaggacctg aaagcaagat atggcccacc ctggattgcg caaagctgcg atttttatcg
     2761 attgcctaga cgccaagacc gcggacttag tactggatcg ccttccgcgg gagtacgcgg
     2821 atcaagtccg aagtacttgg atggccctcg gacgaatccc ggctgaagag cgggaacagg
     2881 tcattcgaga gttcatgcaa acaacggccc aaccatcgcg cgaaacaacg ttaaccagca
     2941 cgcctcccca gtgggaaggc acagaattga tactccagtc gtcaatgaat gcaccggcaa
     3001 agaagaacga agacactgag ggatcaccac cctttcggct attgcgggag gccgaggccg
     3061 ataaactcgt ggggattctt cagggtgaac gccctcagac gattgccttg gtcttggcgc
     3121 acttgccgcc acaccaggcc ggggccgtgc tggtccggtt tcctcccgcg atgcaggtgg
     3181 acatcatcag gtgcttgatc agcctcgaag aaacagatcc tgaggtcctt cgagaagtcg
     3241 atcgtgcctt ggagtccaag ctatctcagt tggtggccat tcaacgacga cggattgccg
     3301 ggatcactgc ggtcaatggg attgtgcatc aatctcagga gcatgttagt cagcagattc
     3361 tggataatct cgcaaacttt gctcccgatt tggcggagcg actctcgccg attccaaaga
     3421 tcgatttcaa
//
LOCUS       DAOPTR010000348         3423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22033_length_3423_cov_3.641924, whole genome shotgun sequence.
ACCESSION   DAOPTR010000348 DAOPTR010000000
VERSION     DAOPTR010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22033_length_3423_cov_3.641924"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..1044
                     /locus_tag="PKI05_07675"
     CDS             244..1044
                     /locus_tag="PKI05_07675"
                     /inference="COORDINATES: protein motif:HMM:NF025186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOA52121.1"
                     /translation="MPHTISVKEKDAGPMGVPQVQSDLDILDCLRNAGPMTVAELAER
                     FSVTPTAIRQRLNRLHAEGLIERQASRAGRGRPHYVYGITGKGRQTAGANYTDLAIAL
                     WGAIRSIDDPKVRAHVLTAAAHEMARMYWRQVKGLTVSERMAEVGRLLSQKRIPFSVE
                     ATGETGSGSNLQATSRPSVASPGDATASRGTRLPILVGQACPYPDLAEIDGQICQWER
                     LWLSELLGIEVHLTQCRREGGSECRFQAAENICSDSDNRWERVQAAST"
     gene            1118..1720
                     /locus_tag="PKI05_07680"
     CDS             1118..1720
                     /locus_tag="PKI05_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOA52122.1"
                     /translation="MTQPWIEGRFEEGLVITTVEQAINWARQGSLWPLTFGLACCAIE
                     MMATGAGRYDIDRFGAGAFRGTPRQADLMIVAGTVTFKMASRVRRLYEMMPDPKYVIA
                     MGACTLGGGPYFEWGYHVVKGVDLIVPVDVYVPGCPPRPEALLEGLMRLQEKIASRKI
                     TKPPASGFEERLPLPQHTGYVDVPESALVFHHQKVKKGSA"
     gene            1741..2556
                     /gene="sufC"
                     /locus_tag="PKI05_07685"
     CDS             1741..2556
                     /gene="sufC"
                     /locus_tag="PKI05_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOA52123.1"
                     /translation="MSPETLSVIDLHVSVEGKPILKGVNLTIRRGEIHALMGPNGSGK
                     TTLGLAIMGHPRYEITRGRIELDGEDITQLEPHERARRGLFMAFQRPVAIPGVRLADF
                     LRHAVSNVRNPARKEGEELIPMREFRKELKEKMDLLRMDAEFARRYVNDGFSGGEMKR
                     AEILQMAMLRPKFAILDETDSGLDVDAVRLASQSIAEIGGRQMGLLIITHHERLLEHN
                     PPDVTHVLLGGRIVETGGPELAERLHREGYDQLRQKYPEAAAEEAALAGQGKQ"
     gene            2675..>3423
                     /gene="sufB"
                     /locus_tag="PKI05_07690"
     CDS             2675..>3423
                     /gene="sufB"
                     /locus_tag="PKI05_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOA52124.1"
                     /translation="MMAEKVDVIGEINKYDFRVDERYVFKAQKGLNRDIVAEISAMKN
                     EPQWMRDFRLKALEIFESKPMPTWGGNIDIDFQDIYYYIKPTERPGHSWEELPEEIKK
                     TFDRLGIPEAEKRFLAGVKAQYESEVVYGSLREDLAKRGVIFVDMDTAVREYPDLVRQ
                     YFSTVIPPTDNKFAALNSAVWSGGSFIYVPPGVNVEFPLQAYFRINAAQMGQFERTLI
                     IVDEGASCHYVEGCTAPMYSSESLHAAVVEV"
BASE COUNT          769 a          837 c         1081 g          736 t
ORIGIN      
        1 ttcgcttatg gcaaccaaat ttgccgttca aggtgtccgg tctgggctcc acatgcaacg
       61 aggagccgaa aagtcggctt tttgacggat tttgaaggta gaacagcgct tatttataca
      121 attagacttg tctaaattgc gtcaatccct tacgatgtta acgaggcact tcctcgggtg
      181 accacgaaaa aggcggtcca agtaccacga tggggtggtg cgacaccacc gacgatttag
      241 tcggtgcctc acacgatttc ggtgaaggaa aaggacgccg ggccgatggg tgttccccaa
      301 gtgcaaagcg acttggatat actggactgt ttgcgaaacg caggccctat gacggtggcg
      361 gagctcgcgg aacggttttc cgtcaccccc accgcgatcc gccaacgact caatcgtttg
      421 catgccgaag ggctgatcga gcgccaggcc tcacgcgcag gacgtggtcg gccgcattat
      481 gtctacggga tcactgggaa gggacgacaa acggccgggg ccaactatac ggacctagca
      541 atcgccttgt ggggggcgat tcgatcgatt gatgacccca aggtacgagc acacgttctg
      601 actgccgccg cccacgaaat ggcccgcatg tactggcggc aagtcaaggg actgaccgtt
      661 tcggagcgta tggccgaggt tggccgcctc ctgtcgcaaa aacggatacc tttttcggtt
      721 gaagcaacgg gcgaaacggg gagtggctcg aatctccaag ctacgtcccg tccgagtgtg
      781 gcaagccctg gcgatgcaac cgcgtcccgt ggaactcgat tacctatcct ggttggccag
      841 gcctgcccat atccggactt ggcggagatt gacggtcaga tctgccagtg ggagcgactt
      901 tggttgagtg agttgttggg gattgaggtt catctgacgc aatgccgacg ggagggtgga
      961 agcgaatgtc ggtttcaggc tgcggaaaac atttgctcgg atagtgacaa tcgctgggaa
     1021 cgtgtacagg cggcgtcgac ttgaggtata gtgaatgatt ggtttatttg acgttggagc
     1081 gcggcggagc ctagggaacc cgagacggta acgagacatg acgcagcctt ggattgaagg
     1141 acggttcgaa gaagggcttg tcatcacaac ggttgaacag gcaattaact gggcccggca
     1201 agggagcctt tggcccttga cattcggcct ggcctgttgc gccatcgaaa tgatggcaac
     1261 tggggcaggc cgctacgata ttgatcggtt tggggcgggg gcctttcgtg ggacgccgcg
     1321 ccaggctgac ttgatgattg tggcggggac ggtgacgttc aagatggcca gccgtgtccg
     1381 gcggctctac gaaatgatgc ccgacccaaa atatgtcatc gccatggggg catgtacact
     1441 tggtggggga ccgtactttg agtggggata ccacgtagta aaaggtgtgg acctgattgt
     1501 gccggtggat gtttatgttc cgggttgtcc gccacgtccc gaagcccttt tggagggatt
     1561 gatgcggctt caggagaaga tcgcgtcgcg gaagatcacg aagccgcccg cgagcggttt
     1621 tgaggaaagg cttccgctgc cgcagcatac aggttacgtg gatgttccgg agagcgcact
     1681 ggtcttccat catcaaaaag tcaaaaaagg ttcagcatag atcttttggg aaaggcaaag
     1741 atgagtccgg aaaccctttc tgttatcgat ttgcatgtca gtgttgaggg aaagcccatc
     1801 ctgaaagggg tgaatttgac cattcgccgc ggtgagatcc acgccctgat ggggcccaat
     1861 ggttcgggca agacgacgct tggcctagcc attatgggcc acccgcggta cgagatcacc
     1921 cgcggccgca tcgagttgga tggtgaagac atcactcagt tggaaccgca tgaacgcgcg
     1981 cggcgtgggt tattcatggc atttcagcgt cccgtggcga taccgggtgt gcggttggcc
     2041 gatttcctgc gtcatgcggt gtcgaacgtg cgtaatccgg cccgtaagga aggtgaggag
     2101 ctcattccga tgcgggagtt ccgcaaggaa ttgaaggaga aaatggattt gctgcggatg
     2161 gatgcggagt ttgcccgccg ctacgtcaac gacggttttt ccggcggtga gatgaaacga
     2221 gccgaaatcc tgcaaatggc gatgttgcgt cccaagttcg ccatcttgga cgaaaccgac
     2281 agcggtttgg acgtcgatgc agttcggctg gccagccaaa gcattgccga gatcggtggg
     2341 cggcagatgg ggctcttgat catcacccac catgaacgtc tattggaaca caaccctccc
     2401 gatgtaacgc atgtcctttt gggtgggcga attgtcgaga cgggcggtcc agagttggcg
     2461 gaacgactcc atcgtgaggg ttacgatcaa cttcgccaga aataccctga ggccgctgcg
     2521 gaagaagcgg cattggcggg ccagggaaaa cagtgacttc ccgtgatgtt aacaacccat
     2581 gagcgtgtga tgcgacggtc cggctggatg ccagtacgac atcggaggga ccggccttga
     2641 gaagcaggcc gggagctagg ctttcggaga gataatgatg gctgaaaaag tcgatgtgat
     2701 cggcgaaatc aataagtacg actttcgggt cgatgagcga tatgtcttca aggcccaaaa
     2761 aggcctcaat cgcgacattg tggccgaaat ttcggccatg aagaacgaac cgcagtggat
     2821 gcgggatttc cgcctgaagg ccctcgaaat ctttgaatcg aaacccatgc caacctgggg
     2881 cggaaatatc gacatcgatt tccaagatat ctactactac attaagccga cagagcggcc
     2941 aggacattcg tgggaagaat tgccggaaga gatcaagaaa acatttgacc gactcggcat
     3001 tccggaggcc gaaaagcgat ttttggcggg ggtcaaggcc caatacgaaa gcgaggtcgt
     3061 ctatgggtcg ttgcgcgaag acctggccaa gcgcggtgtt atcttcgtgg atatggatac
     3121 tgccgtccgc gagtacccgg atttggtgcg gcaatacttt tcgacggtca ttccgcctac
     3181 ggataacaaa ttcgcagcgt tgaattcggc ggtgtggtct ggtggctcgt tcatttacgt
     3241 cccgccgggg gtgaatgtgg aatttccact tcaagcctat tttcggatca acgccgcgca
     3301 gatggggcag tttgagcgaa cgttaatcat cgtcgatgaa ggggcctctt gccactacgt
     3361 ggaaggttgt acggccccga tgtacagttc ggaaagtctc cacgccgcgg tggtcgaggt
     3421 gat
//
LOCUS       DAOPTR010000349         3422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22044_length_3422_cov_3.696763, whole genome shotgun sequence.
ACCESSION   DAOPTR010000349 DAOPTR010000000
VERSION     DAOPTR010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22044_length_3422_cov_3.696763"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..1376)
                     /locus_tag="PKI05_07695"
     CDS             complement(234..1376)
                     /locus_tag="PKI05_07695"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOA52125.1"
                     /translation="MPIFTRYVTSELLKIFLTALLIMTLLLALGIGAREGISRGLPPL
                     LVMRMIPYLLPEILGITIPIAVLLAVSQVLGRMAGSNEIIALRAAGVSPSLVVRPTVI
                     FAFFVSLFTVWTYDLAATWGRPGARRVVVESVEQIAYGMLRTRKSYSSQKFSLVVKDV
                     VGKRLISPIITVQVQPESPAITLTAEEAELFTDRKAGALTIVCRKPVARIEGEATVTL
                     PETYWYPIPFETSYEPLHRDWLAMSEIPAAIRKLEGDARQLKLLLTEGGASEEQRRRW
                     NEQLQNLELQIRKLQTEPYRRWANGFSSLCFVLLGCPLAMYRRSENFLAIFFLSFLPI
                     VIVYYPLLMLANGLTSSGILPAVGFWIANLGIAIPGVVLLYYYCET"
     assembly_gap    1799..1898
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1899..>3422
                     /locus_tag="PKI05_07700"
     CDS             <1899..>3422
                     /locus_tag="PKI05_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52126.1"
                     /translation="LEALKSRGYYDTAVEYLDMLASDPTCPAELKEVIDYEAGVALVA
                     AAQTARNPQKRDQALKESLERLRKFIQEHPQHALADAARAQLGQGLVEQAKALLQGIE
                     TKKPDEVSRLRGEARKLIEQAKQHFDAYEKSAYERAKQFQGKVIDARNEQLAREREQA
                     YAEYLKARMFTIGAIYQLAQTYGKEEKQRAELLKQAAGEYEKFYEKYRDFSAGFFARF
                     YQGICAQELGQDNVAIDIFKETLTVPGDLPEIQRLRTEALARLIQIYRKQNKHQDIVS
                     AAVSWEEAVRPDVRNTDWGLEILYHGALSAIELAKSAEKNKEGRTAQQQRALAQRFLK
                     IVASVPGQYRLDAQSKLGELLGEERTIEDSPVVDFADGKARGEAAWGRFLAAMAQLDK
                     GEGDRTQIRQEANRARDQAISFYQKAIALYKDQPIEELNAIRYQLIYLFWDAGALEKS
                     AVLSEFLARNYPQNAGSQKAVEIAVKVYRNLFAQARKDGQDGSFELEKMRQLTEYALS
                     "
BASE COUNT          854 a          863 c          900 g          705 t
ORIGIN      
        1 gggagggcaa ccggaggtca tccttagaaa cggggctaag aaaattgccg accaactagg
       61 cattgaaaag attcagattt cgatctccca ttgtcgcaca catgccgttg cgtttgcggt
      121 ggccgtggga gactaattgg aaaaattggg caagttgatc ttgtcgtatt tcacctcgtc
      181 ggataaccgg tcacagtttg cagtgaccga gccgatcatt gatcttttct cgactatgtt
      241 tcgcagtagt agtaaaggag gacgacgccc gggatcgcga tccccaggtt ggcaatccaa
      301 aagccaacgg ccggcaggat tccagagctt gttaagccgt tggccaacat caaaaggggg
      361 tagtaaacaa tcacgatggg aagaaagcta agaaagaaaa tcgccaggaa attctcgctg
      421 cgacgataca tcgctaatgg gcaacccaaa agtacaaaac aaaggctgct aaacccgttg
      481 gcccaccttc ggtagggttc ggtctgcaac ttgcggatct gtaattccaa gttctggagt
      541 tgttcattcc atcgtctgcg ctgctcctct gatgcgcccc cctccgtgag aagcaatttc
      601 aactgccgtg catcgccttc caatttgcgg atagcagccg gaatctctga cattgccaac
      661 caatcacgat ggagaggctc gtaagaggtc tcaaagggga tcgggtacca gtaggtttcc
      721 ggcagggtaa cggtggcctc tccttcaatt cgggcgacag gctttcggca aacaattgtc
      781 aaggcacccg ccttccgatc cgtaaacaat tctgcctcct cggccgtcag cgtgatcgca
      841 gggctttcag gctgaacctg aacagtgata atggggctga tgagcctttt gcccacgacg
      901 tctttgacaa cgagggaaaa cttctggctg ctataactct ttctcgttcg aagcatgcca
      961 taagcgatct gctcaacgga ttccacaacg acccgccggg cacctggtcg gccccacgtg
     1021 gccgccagat cataggtcca gaccgtaaat agactcacaa aaaaggcaaa aatcaccgtc
     1081 ggtctgacaa caagcgaagg cgacacgcca gcggcccgta gcgcgatgat ctcattgctt
     1141 ccggccattc gccccaagac ttggctgaca gccaagagga cagcaatggg aatcgtgatc
     1201 ccgagaatct ccggaaggag gtagggaatc atccgcatca cgaggagggg cggcaatccc
     1261 cggctgatgc cttccctggc cccaatcccc aacgccagta acagcgtcat aatcagcaac
     1321 gctgtcaaga aaatcttcaa gagttccgag gttacatacc gtgtaaaaat aggcatccct
     1381 ggattcctaa cggtatccaa gacgtttctc gaccacacaa aatggccata taggttacca
     1441 ttctctccga aaaacgtaaa tcccaatcgc gtctgcaatt agccgttatt gcacgatcgg
     1501 aacatatttc gactccaggg atgagtttca ggatatcgcc cgcgacactg tggatcgacg
     1561 atgagagatt agactgtgcg gaagtaatag cccccttaga ctactggact ggagggctga
     1621 cagtttgtaa aagataggta acggtacatc atcccttgcg gaaaagggga gtgacccttt
     1681 gctggttccg gatggaggca atacgttcga tgatttggcc gcgattgccg gttgctttgg
     1741 cgcttgttgg cagttttgca tactcgtctc tcctggctca gacttccggt acacctcann
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt ggaagccctt aaatctcgtg
     1921 gatactacga cacggcagtc gagtacctgg atatgttggc ttccgatccc acgtgtcccg
     1981 ccgagctgaa ggaggtcatt gattacgaag ccggggtggc tttggtagcg gcggcccaaa
     2041 cggcccgcaa tccgcagaaa agggatcaag ccctcaaaga gtcgcttgag cggctccgca
     2101 aatttatcca ggagcatcct cagcacgcac tggccgacgc agcccgtgcc caattgggcc
     2161 agggattggt ggagcaggca aaggccctcc ttcagggtat tgagacgaaa aaaccggacg
     2221 aagtcagtcg tttgcgtggg gaggcccgca aactgatcga gcaagccaag cagcatttcg
     2281 acgcttacga aaagagtgct tatgaacggg ccaaacaatt ccaaggtaaa gtcattgacg
     2341 cacggaatga acaattggcc cgagaacgcg aacaggccta cgccgagtat ctcaaggccc
     2401 gcatgttcac gattggggct atttaccaac tggcccagac gtacggtaag gaggagaagc
     2461 agcgtgcgga actcctcaag caagccgcag gcgaatacga aaaattttac gaaaaatatc
     2521 gtgacttctc ggctgggttt tttgctcgat tttaccaggg tatctgtgcc caggagcttg
     2581 ggcaggacaa cgtggccatt gacatcttca aggaaaccct cacggttccc ggtgatttgc
     2641 cggagattca gcggctccgc accgaggccc tggcacggtt gatccagatt tatcgaaagc
     2701 aaaacaagca tcaagatatc gtttctgctg cggtgtcgtg ggaggaagcg gtccgcccgg
     2761 atgtgcgaaa caccgattgg gggttagaga tcctctatca cggcgccctt tctgccattg
     2821 aactcgccaa gagtgctgaa aagaacaagg aaggccgaac agctcaacaa cagcgggcac
     2881 tggcccagcg attcctcaag atcgttgcca gtgttccggg acagtaccgg ttagatgccc
     2941 aatccaaact gggagaatta ttgggcgagg agagaaccat cgaggattcc cccgtcgtcg
     3001 attttgccga tggcaaagcg cgcggcgagg cggcgtgggg acgttttctg gccgccatgg
     3061 cacaactgga taagggtgaa ggcgatcgta cccaaattcg tcaggaggcc aatcgggcaa
     3121 gagaccaagc cattagcttt taccaaaagg ctatcgcttt gtataaggat cagccgatcg
     3181 aagaattgaa cgctatcagg taccagctca tttacctatt ctgggatgca ggagccctcg
     3241 aaaagtcggc tgtcttaagt gagttcttag cccgcaacta tccacaaaat gcgggatccc
     3301 aaaaggccgt cgagattgct gtcaaggttt atcgcaatct ttttgctcaa gcgcggaaag
     3361 atgggcaaga cgggtccttt gaattggaga aaatgaggca attgacggag tacgcactct
     3421 cc
//
LOCUS       DAOPTR010000350         3418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22074_length_3418_cov_3.082664, whole genome shotgun sequence.
ACCESSION   DAOPTR010000350 DAOPTR010000000
VERSION     DAOPTR010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22074_length_3418_cov_3.082664"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="PKI05_07705"
     CDS             <1..1071
                     /locus_tag="PKI05_07705"
                     /inference="COORDINATES: protein motif:HMM:NF019606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HOA52127.1"
                     /translation="GLVDWPDIVIGGHEIRDVSLAEESRAFASVNRTFERQHVDLIAD
                     ALESIEQQIRYGTVFGADQMIAQLASPNVPRDRSAREAIERIAADLKDFKSRHNLRSL
                     IVINVASTEPPVDPSQFPAKWSELEQLLAEQPDPGLPTSTLYAIAAFETGCPYINFTP
                     SLGPALPALDELAQHNNVPYMGCDGKTGETLMKSVLAPMFRARNLRVLSWVGHNIFGN
                     LDGKILDDPRHKSSKVQSKDRVVSGVLGYHPQTLVSIEYIEGLGDWKTAWDHVHFQGF
                     LGVPMVLQFIWQGCDSILAAPLVLDLCRLTERAHRIGIVGLMPFLASFFKSPYDVKEA
                     DFFKQFQMLEQWIESVSHSHPS"
     gene            complement(1097..1894)
                     /gene="lptB"
                     /locus_tag="PKI05_07710"
     CDS             complement(1097..1894)
                     /gene="lptB"
                     /locus_tag="PKI05_07710"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOA52128.1"
                     /translation="MRLFTGRAVQPIAQNGPHIRAVPGAILAACGLVKTFGKRRVVDG
                     VSFEVWPGEIVGLLGPNGAGKTTSFRMTCGLIRPDAGQVFLQGQDVTHWPMHRRAREG
                     GMGYLAQESSVFRKLTVEENLLGVMELLGFDRKLRLRRCRELLEQFDIVQLAKAKAGG
                     LSGGEKRRLEIARALVSNPRIILLDEPFTGIDPVTVDAIQEIIRNLRAQGISILITDH
                     QVRETLQVTDRSYVIRAGRVLCHGTPEEVIANPEARKSYFGEDMQLN"
     gene            complement(2146..>3418)
                     /locus_tag="PKI05_07715"
     CDS             complement(2146..>3418)
                     /locus_tag="PKI05_07715"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOA52129.1"
                     /translation="TNFIKRLVGLPNETVRIYRGDVYIRPAGSDQFAIARKPPEKILA
                     LLQPVYMDDFQSPELKAAGWPDRWQPEVGQQGLWEIGENPRQYRVTAPEDMEVWLRYQ
                     HIVPSWEHWQAILEGNPIDAKQVSPQLISDFMAYNTNQSDESDYGPHTLRTVRIPPRG
                     DQLGLHWVSDLAVECEADMESDGGEIILELGAGKNRYQARLNLADGVARFIGMGLRSF
                     SEEAPIHIHGRGHYQLRFANVDHQLVLWVNGRPITVEYEAVWSGPTLDDRDRPARIGV
                     RGGTVTIRHVRLFRDVYYIAHDRRSGLLMHDFDWERSPYRSLIPSPFAMDQFRQMVAE
                     VMSDPTQWEFFQYANHLEFALGPDQFFVLGDNSPQSEDGRFWTKKSYVQRELLVGKAL
                     AVIWPHSLDRIPGTKIPIRFFPNFWRMKVVR"
BASE COUNT          784 a          942 c          888 g          804 t
ORIGIN      
        1 ggacttgttg attggccgga catcgtcatc ggaggccacg aaatccgcga tgtatctttg
       61 gcagaggaat cccgggcatt tgcgtcggtc aatcgcacgt ttgagcgaca acacgtggat
      121 ttgattgccg acgcattgga gagtattgaa cagcagattc gttatggaac ggtctttggc
      181 gccgatcaga tgattgccca actcgcttcg cccaatgtgc cacgtgatcg ctctgccagg
      241 gaggccattg agcggattgc cgcggatttg aaggacttca aaagtaggca caacctccga
      301 tcgcttatcg tgatcaatgt cgcgtcaacg gaaccaccgg ttgatccgtc gcagtttccg
      361 gcaaaatggt cggaactgga acaattgctg gccgagcaac ccgaccccgg tctgccgaca
      421 agtaccctat atgcgattgc tgcgttcgaa acggggtgtc cttatatcaa cttcacgccg
      481 tctctaggac cagctttgcc ggcactggac gagcttgccc aacacaacaa tgttccctac
      541 atgggctgcg acggcaagac gggcgaaacg ttgatgaaaa gtgttcttgc gccgatgttt
      601 cgggcaagaa atttaagggt tttgagttgg gttgggcaca atatctttgg gaatctcgac
      661 ggcaaaattc tggatgatcc gcgacacaag tcaagtaagg tccagagcaa ggaccgcgtt
      721 gtttccggtg tactggggta ccacccgcaa acgttagtca gcatcgaata cattgagggc
      781 cttggtgatt ggaagacggc atgggaccac gtgcactttc agggatttct cggagtgccc
      841 atggtcttgc agttcatttg gcaaggttgt gactcaattc tggctgcccc actcgtgctt
      901 gatctttgtc gtctcacgga aagggcacac cggatcggca ttgtggggct aatgcctttc
      961 ctcgccagct ttttcaaaag cccctacgac gtcaaagaag ccgatttctt caagcaattt
     1021 caaatgcttg aacagtggat tgaatctgtc agccattcgc acccctcatg atttcgccag
     1081 ttggggctgg acagagttag tttaattgca tgtcttctcc gaagtaggat ttcctggcct
     1141 caggattggc gatcacttct tcgggagtcc cgtgacacaa gacccttccg gcccgaatga
     1201 cgtagcttcg atcggtcacc tggagcgttt ctcgcacttg gtgatccgta atcagaatag
     1261 atattccttg agcgcggaga ttgcgaatga tctcctgaat ggcatccacc gtcactggat
     1321 cgatcccggt aaagggttca tcgaggagga tgatacgggg attagagacg agtgcccggg
     1381 caatctcaag tcgtcgcttt tcaccgcccg acaacccgcc agctttggcc tttgccaatt
     1441 gcacaatatc aaattgttct aaaagttcac gacagcgtct taaccgcagt ttgcgatcaa
     1501 accccagcaa ttccatcacc ccaagaaggt tttcttcgac cgttagctta cggaagacgc
     1561 ttgactcctg agcaagataa cccataccgc cttcgcgcgc ccgccgatgc atgggccagt
     1621 gagtcacgtc ttgtccttgg agaaagacct ggccggcgtc gggccgaatc agcccgcacg
     1681 tcatgcggaa acttgtggtt tttccggcac cattcggtcc aagaagaccg acgatctcgc
     1741 ctggccacac ctcaaaactt acgccatcga ccacgcgccg ttttccgaag gtcttgacca
     1801 atccgcaggc tgccaagatg gcaccgggaa ccgcccggat atggggacca ttttgagcga
     1861 tgggttggac cgcacgtcct gtaaacagcc tcatatgtcg ctccgtctga ttagaaagct
     1921 ctccgtcctt cgtccacacc aaataagaac ctatcccata atcccccctc gcccatttgg
     1981 cgggagaggg ggtcgggggt gagggtggat ggaatcgctg ccggttttct caatcgattc
     2041 cgacgttcgc ttcccccgcc ccaaccctct cccagaggga gaggaggatt ctttgggata
     2101 gtctctaacc tgctacggta actgcttccc ctgataatca agtgattatc gtacgacctt
     2161 catcctccaa aaattcggaa agaagcgaat gggaatcttt gttcctggaa tccggtccag
     2221 ggaatgtggc cagatcacgg ccaaagcttt cccaacaagg agttccctct gcacgtagga
     2281 ctttttcgtc cagaatcggc catcttcgct ttgcgggcta ttatcaccga ggacaaagaa
     2341 ttgatctggt ccaagagcga actccagatg attggcgtac tggaagaatt cccattgcgt
     2401 tggatccgac atgacctcag ctaccatctg gcgaaactgg tccatggcga aaggactggg
     2461 gatcaacgag cgatacggcg accgctccca gtcaaaatcg tgcataagga ggccggaccg
     2521 gcgatcatgt gcgatgtagt acacatcacg aaagagacgg acgtggcgga tcgtgacggt
     2581 accacctcgg acgcctatac gagccgggcg gtcacggtca tccagtgtcg gccctgacca
     2641 gactgcctca tattccacag tgataggccg accattgacc caaagtacca attggtgatc
     2701 gacattggca aacctgagtt ggtaatgtcc gcgtccgtgg atgtgaatag gtgcctcttc
     2761 agaaaagctt cggagcccca tgccaatgaa cctcgcaacg ccatctgcca ggttcaaccg
     2821 tgcctgatag cgattttttc ctgctcctag ctccaagata atctctccgc catcgctttc
     2881 catatcggct tcacactcaa ctgctagatc gctgacccag tgcagcccaa gttgatcacc
     2941 acgcggcgga atacgaacgg ttcgaagagt atgagggccg tagtcgctct cgtcactttg
     3001 gtttgtatta taggccataa agtcgctaat caattgcgga gagacctgtt tggcatcaat
     3061 gggatttccc tcaaggatcg cttgccagtg ttcccacgaa gggacaatat gctggtagcg
     3121 aagccacact tccatgtcct caggggccgt cactcgatat tgccgcggat tttccccaat
     3181 ttcccaaaga ccttgttgac ccacctccgg ttgccaccga tcaggccatc cggctgcctt
     3241 gagctcaggt gattgaaagt catccatata gaccggctgc aatagggcca gaatcttttc
     3301 cggcggtttt ctggcgatcg caaactggtc actgcccgcc ggccggatat agacatcccc
     3361 ccggtaaatg cggacggtct cattgggcaa gccaacaagc cgtttgatga aatttgtt
//
LOCUS       DAOPTR010000351         3416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22089_length_3416_cov_3.927700, whole genome shotgun sequence.
ACCESSION   DAOPTR010000351 DAOPTR010000000
VERSION     DAOPTR010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22089_length_3416_cov_3.927700"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="PKI05_07720"
     CDS             complement(<1..1071)
                     /locus_tag="PKI05_07720"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOA52130.1"
                     /translation="MVWRWSLVEWVTLVMACAGLSSVVCHAEERPYRRISLATYQDKM
                     EAAWIGQMVGVGWGGPTEFRAVGRILREDELPAWKPEMVNQFNQDDLYVEMTFLRTLE
                     EYGWEVDIRQAGIDFANSRYRLWHANRAGRDNLRSGIAPPDSGHPQFNKHADDIDYQI
                     EADFSGIIAPGLPNTVIKLGNIFGRLMNYGDGLYGGQFVGGMYAEAFFENDPLKIVEA
                     GLRCIPAESQYAECIRDVIAWWKENPQDWQATWQKIEEKYNRNPRYRRASCMKELGNI
                     DAKINGAYIVMGLLFGKGDIDQTVIISTRCGQDSDCNPSNAAGILFTTIGAKQLPERF
                     TSKLNREAVFSHTAYNFDRLVEV"
     gene            complement(1528..1851)
                     /gene="rpmB"
                     /locus_tag="PKI05_07725"
     CDS             complement(1528..1851)
                     /gene="rpmB"
                     /locus_tag="PKI05_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOA52131.1"
                     /translation="MSQKCEICGKTPAIGKRVTTRGKAKYLGGVGTKITGISPRQFRP
                     NLQRIRITDENGAPRRVRVCVQCIRSGRVTKRIKNRPFALPADAAALLAAAREKKAQK
                     KGKKS"
     gene            2744..3142
                     /locus_tag="PKI05_07730"
     CDS             2744..3142
                     /locus_tag="PKI05_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOA52132.1"
                     /translation="MSDFNLPEPGAEQQEEKTAQQPTPEAQAQQPAGPPRVTVQIDDS
                     KIVASYANFCRVTGTPEEVIIDFGLNPQPIGIPSQPIAVSQRIVMNFYTAKRMLHALQ
                     LTIQRHEATFGVLETDIQKRVRPMPPRPNA"
BASE COUNT          724 a         1045 c          905 g          742 t
ORIGIN      
        1 gacctcaacc aaccggtcga aattgtatgc cgtatgactg aacaccgcct cgcgattgag
       61 cttgctcgta aaccgttcag gcagttgctt ggccccgatc gtggtaaaga gaattcccgc
      121 cgcgttcgag ggattgcaat cggaatcctg accacaccgg gtggaaatga tcaccgtttg
      181 gtcaatatcg ccctttccaa aaagcaaccc catcacgatg taggcaccat tgatcttggc
      241 gtcgatgttc cccaattcct tcatgcagga cgcacgccgg tatcgcggat tgcgattata
      301 tttctcctcg atcttctgcc aagtggcctg ccaatcttgc ggattctcct tccaccaggc
      361 gatgacatcg cggatacact cggcatattg gctttccgcg gggatgcacc gcagaccggc
      421 ctcgacgatc tttaaggggt cattttcaaa aaaggcctcc gcatacattc cgcccacaaa
      481 ttgcccgccg tacagcccat ccccgtagtt catcagccgt ccgaagatat tgcccagctt
      541 aatcaccgtg ttgggcaatc ccggggcgat gatgcccgaa aaatcggcct caatctgata
      601 gtcgatgtcg tccgcatgct tattgaactg aggatggcca gaatctggcg gggcaatgcc
      661 cgatcggaga ttgtcccgtc ccgcccgatt ggcatgccac agccgatagc gactgttggc
      721 aaaatcgatc cccgcctgcc gaatgtccac ctcccagccg tactcttcca gagttcgcaa
      781 aaaggtcatc tcgacataca aatcatcctg attgaactgg ttgaccattt cgggcttcca
      841 cgccgggagc tcgtcctccc gcaggatccg ccccaccgcg cgaaattctg tggggccacc
      901 ccaccccaca ccgaccattt ggccaatcca ggctgcctcc attttgtctt gatacgtcgc
      961 cagcgaaatt cgccgatacg gcctttcctc cgcatgacag accacgctcg aaagccccgc
     1021 gcacgccatc actagggtta cccattcgac caacgaccat cgccacacca tggaaagtcc
     1081 tcccttggtt tgcacaggct caccttgcag acacaacccc caccgagcgg ctgacttcgg
     1141 caggaccgcc gatttccttc cgacggcggt ctattatatg cccctcgggc agccaccgca
     1201 aacgactcag tggtaggcaa tcccctagag gcccagccca ctttgacgat ccgcccggaa
     1261 cggatgaacc cagcggcgtg gccggccgag agggcacgtc acgcttcctt caacaccgcg
     1321 atggaaaaag cggaggccta aaggcgacta tctgagaagg aaaccgcggc agtcgccaag
     1381 caaacgctgc tttcgcgaca agctatgttt ccgcgatgcg gagaacgaga gagatgttca
     1441 cttgtacata tcagcccaga aaaaactagc caatccgggc tagatcagac accccggttc
     1501 ttttcagaag cggcgaacac cgctcgccta cgatttcttg ccctttttct gggctttctt
     1561 ttcccgggca gcggccagaa gggcggcggc atcggcaggg agtgcaaacg ggcggttctt
     1621 gatccgcttg gtaacccgcc cactgcgaat acactgcacg caaacccgta cccgccgtgg
     1681 ggcaccattc tcatccgtga tacggatccg ctggagattc ggtcggaact gacgcggcga
     1741 gataccagtg atctttgtac ccacacctcc gaggtacttg gctttacccc ttgttgtgac
     1801 ccgctttcca atcgctggcg tttttccgca gatttcgcac ttttgactca tcgttatcag
     1861 tcccagctta ttcaagggtg gcgctgatct cgcgcctatc tcttcctttt gtcctcttgt
     1921 cctctggcaa acagacgatg gcagggaaaa cactcaatat actgaatccc cctgcgatga
     1981 caagtggagc gggttggatc gagcaccaac cgtaggagct gagggccggg cccccgtgcc
     2041 cgccctagaa aaccggacgc tacgccaacg attgccaacc cgcggtccct cccgggattc
     2101 tgggggtaac cacggggggt tgcccctacg gttagaatcg agtctaagcc cctaggttgg
     2161 cgatagacag cttttccctc gttcgcctgg ctgcggaaaa agattcgtgg gttactttac
     2221 cttgacgtgg agcctacgcg ggaaagcagg gccctccacc cggcgcgcgg agccggtaat
     2281 ctactgagtg ttgctctgga agtgttgcgg aagaatagct cccgctgtaa acagcaaaga
     2341 ggttacacaa tggcatcgcc ctataataac gcaccttaac cccaccaaag ggggggcaag
     2401 agtgctgttg tgcgtcactt attcagcaac cctgccacat tgctatggcg ttatgattat
     2461 gtcaaattga tgatatcggc aggttgtgca gcatctcgga gttggctcat ctgtactgcc
     2521 gatctggagc ggttccgggc cgcttcgtcg tttcgtgatc agcacattct cttgggtatg
     2581 ctatattcca ctaggggttg acacctgaac tggtcgtttt ggccccagca gctcaaagca
     2641 cgttttttga gcgatgggga tgcaggcaag ggtcggccag cggttggtcg caccctatgg
     2701 ataggtcgtg tttgagggaa ttttttgacg aaaggaatga gctgtgtcgg acttcaatct
     2761 tcctgaaccc ggtgccgagc aacaagagga aaagacggct caacagccga ccccagaggc
     2821 tcaggcccag caacccgcgg gaccgcctcg cgtgaccgtg cagatcgacg atagcaaaat
     2881 cgtcgccagc tacgccaatt tctgtcgcgt tacggggaca ccggaggagg tcatcatcga
     2941 ctttggcctc aacccgcaac cgattggcat cccgtctcag cccatcgcgg tgagccagcg
     3001 gatcgtgatg aacttttaca ccgccaagcg gatgctccac gctttacaac tgaccatcca
     3061 gcggcacgag gccactttcg gcgtgctgga gacagacatc caaaagcgtg tgcggcctat
     3121 gcccccgcgg cctaacgcgt gaacaaccag atgccaagag ccggcgatct tgtgtgggtc
     3181 ctggacacag cgtcccatta ggtcgtggca gaaagaccaa ttcgcccgcc gttttctgat
     3241 ggtgggcgtt ttattttcgt cccggcttag ggtctacccc gtagtcttcc ctcgcccgtt
     3301 cggcgggaga ggggtcgggg gtgagggtgc ctcgaattgc tcgcggtttt ctcaatcgat
     3361 tccgacgttc gcttgccctc accccaaccc tctcccggag ggagaggggg actttt
//
LOCUS       DAOPTR010000352         3404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22185_length_3404_cov_3.062108, whole genome shotgun sequence.
ACCESSION   DAOPTR010000352 DAOPTR010000000
VERSION     DAOPTR010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22185_length_3404_cov_3.062108"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="PKI05_07735"
     CDS             <1..1165
                     /locus_tag="PKI05_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA52133.1"
                     /translation="IGMRYQGTVITLIAQNHGNVVAIADVDRNVREQARASFGSTPAI
                     YEDYRELLDRKDVDVVLIGAPDHWHAKMVIDACRAGKDVYVEKPLTLTIDEGKVIRRV
                     VEETKRVVQVGSWQRSDARYRLAVELARAGRLGKLQRVTCVAGPNPTGGPFPVRPVPA
                     ALNWNLWLGQAPEVPYTEERCHYTFRWWFEYAGGKITDWGAHDIDIAQWAIGELPVEV
                     KGHGKLPNIPNGYNVPIDFGAVVRYPSGVELIVQEEGRVGVLFEGEKGRLFVNRGTIS
                     GSVVDELKDHPFPKDQYKLYDFDDPNRPDRVGKLDAIVNHMANFFDCIRARRLPISDY
                     ESQHRSATTCHLVNLAIRLGRPLKWDAEKEIFVGDDEANQHLSRPQRKGFEVA"
     gene            1193..2191
                     /locus_tag="PKI05_07740"
     CDS             1193..2191
                     /locus_tag="PKI05_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOA52134.1"
                     /translation="MHHPSPRSDRWSHCAGPNRRAFLRTVLKGASAITGVTTLLSWEK
                     WAVAVEPSTADRFSLRYLLASCLYGYADLEEILPEVKKTGASAIDLWPKVHGNQREQL
                     DLLGPDRFEELLRRHGVQLGCLTRYDLGPFALADEIRLAARLKCPLIVTGARGPTGLK
                     DADLRQAIREFVEKMKAILALAEESGVTIAIENHANSLIESPDSMRWLAEAWPSRTLS
                     IAFAPYHLPQDTDLLARLIHDLGDKIAVFYMWQHGKGCMTAMPLEDELLQLPGKGPLD
                     FRPLLAALRDIKYSGYSSIFMHPYPRGRPIRENISLVTSEVLQAKSYLEQCFAELG"
     gene            2229..2804
                     /locus_tag="PKI05_07745"
     CDS             2229..2804
                     /locus_tag="PKI05_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007339880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOA52135.1"
                     /translation="MEKVLIIVGDATETMDTLYPYYRLIEAGFQPVVAAPEKRRYQMV
                     MHEVKPGWTITKEWEGYTIEADIAFRDVKPEEYLGIFFSGGRAPEYIRYDEDLVRITR
                     HFFETNKPIACVCHGVEIPAYAGCVKGRRMATVPKCRFDLEVCGGIFVDAPCVVDGNL
                     VSGRTYHDHGHYIGAWIKLLEAARDAKSIDR"
     gene            2853..>3404
                     /locus_tag="PKI05_07750"
     CDS             2853..>3404
                     /locus_tag="PKI05_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008666778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA52136.1"
                     /translation="MHRIRALFSAESASANCRSIVRINPYYWRMPLIVSLGALGVFIW
                     GARSAWAEVGSPQRPNIVFILADDLGYGDLPCYGRDDLSTPAIDRLAREGVRFTQAYA
                     NGPECTPTRAAFLTGRYQQRIGGLECAIGIGDVGRYDDAMRLALAGKLGLPTEETTIV
                     SLLQNAGYDTVLFGKWHLGYASKF"
BASE COUNT          767 a          839 c          985 g          813 t
ORIGIN      
        1 gatcggcatg cgatatcaag ggacggtcat cacgttgatt gcccagaatc acggcaacgt
       61 tgttgccatt gctgatgtcg atcgaaacgt acgtgagcaa gcccgggcca gttttggcag
      121 tacgccggcc atctatgagg attaccgaga gcttttggac agaaaagatg ttgacgttgt
      181 tctcatcggc gctcccgacc actggcacgc caagatggtc attgacgcat gccgggccgg
      241 caaagatgtg tatgtcgaga aaccgttaac cctgaccatt gacgaaggaa aagtcattcg
      301 ccgagtcgtc gaagaaacaa aacgggtggt ccaggttggc tcctggcagc gaagtgatgc
      361 gcgatatcga ctcgctgtgg aactggcacg ggcaggtcga ttgggcaaac tccagcgggt
      421 gacgtgtgtg gctgggccta atcctacggg aggaccgttc cctgttcggc ccgtgccggc
      481 cgcgctcaat tggaatctgt ggcttggcca agctccagaa gtcccctaca ccgaggaacg
      541 ctgccattac accttccgct ggtggtttga atatgccggc ggcaagatta cggactgggg
      601 ggctcatgac atcgacatcg cccaatgggc tattggtgag cttcccgtgg aggtcaaagg
      661 gcacggcaaa ctgccgaata tcccgaatgg ttacaacgtt ccgatagatt tcggcgccgt
      721 tgtccgctac ccaagtggag ttgagctgat cgttcaggaa gaaggccgcg tgggcgtttt
      781 gttcgaggga gaaaagggac ggctatttgt taatcgcgga acaatttcag ggagtgttgt
      841 tgatgagcta aaggatcatc ctttcccaaa agaccaatac aagctttatg acttcgatga
      901 tcctaaccgt ccggaccgcg tgggaaaact ggatgcgatt gtgaaccaca tggcgaactt
      961 tttcgattgc atccgggcgc gtcggcttcc tatctcggat tatgaaagcc aacatcgtag
     1021 cgcgacgacg tgccatctcg tcaatttagc cattcgactt ggccgacccc tgaagtggga
     1081 tgccgaaaag gaaattttcg tcggagacga cgaagccaat cagcacttat ctcggcctca
     1141 acgcaaagga ttcgaagtcg cctgacatgc tttcgtctga aagggaggga ttatgcatca
     1201 tccgtcgccc cgaagtgaca ggtggtcaca ttgtgcaggg ccaaaccgac gcgcgtttct
     1261 gcggacggtt ttgaagggtg catccgccat taccggcgtc acgacgcttt tgtcctggga
     1321 aaaatgggca gtcgcggtgg agccatcgac tgccgaccgg ttttcgttgc gatatctttt
     1381 ggcctcctgt ctttatggat atgccgatct cgaagagatt ctgcccgaag tgaaaaaaac
     1441 gggggcctcg gccattgacc tttggcccaa ggtccatggt aatcagcggg agcagctcga
     1501 tttgctgggg ccagatcgct ttgaagagtt gctgaggcgt catggcgttc agctaggatg
     1561 tttgactcgc tatgatcttg gtccgttcgc tctcgcagac gaaattcggt tggcagcgcg
     1621 tctcaagtgt ccgctaattg tgacgggcgc tcgcggcccg accgggttga aagatgctga
     1681 ccttcgtcag gccatccgtg agtttgtcga aaaaatgaaa gcaatcttgg cactcgccga
     1741 agagagcggt gtcacgattg ccattgagaa ccacgccaac agcctgatcg agtcacccga
     1801 ttctatgcga tggttggccg aagcgtggcc ttcgcgcacg ctgtccattg cttttgcccc
     1861 ttaccatttg cctcaagaca cagatttgct cgcgcggctg attcatgacc tgggcgacaa
     1921 gattgccgtg ttttacatgt ggcaacatgg caaaggttgt atgacggcga tgccgctgga
     1981 ggacgagctg ttacaactgc cgggcaaggg tcccctggat tttaggccgt tgctcgcagc
     2041 cctccgcgat attaagtatt ctggctacag ttcgattttt atgcacccct atccgcgggg
     2101 aagacctatc cgagagaata tttcactggt cactagcgaa gttttgcagg cgaaaagcta
     2161 cttagagcag tgctttgctg agcttggctg aaaggcttca aaacagttaa ggtagagagg
     2221 aaaccgctat ggaaaaggtt ctcatcattg ttggtgacgc caccgaaacc atggatacac
     2281 tttatccata ttaccggctg attgaggccg gatttcaacc tgttgtcgcc gcaccagaaa
     2341 agcgccgtta ccaaatggtg atgcacgagg taaagccagg atggacgatc accaaggaat
     2401 gggagggata tacgatcgag gccgacattg cgttccgaga tgtcaagccg gaagagtatt
     2461 tgggaatttt cttttctggc ggtcgtgcgc cggaatacat ccgttacgat gaagatttgg
     2521 tgcggatcac gcggcatttc ttcgagacca ataagcccat tgcctgtgtc tgtcacggcg
     2581 tagagatccc ggcttatgcg gggtgcgtga aaggccgacg tatggcgacc gttcctaagt
     2641 gtcgattcga tctggaagtc tgtggtggga tttttgtcga tgcgccgtgt gttgtcgatg
     2701 gaaatcttgt cagtggccga acatatcatg accacggaca ctacatcggt gcctggatca
     2761 aacttttaga agcggcccga gacgcaaaat ctattgatcg ctaggctggt tagaaacact
     2821 ttctaagaaa cacgaaatac ggggagacgc tcatgcatcg gattcgagca cttttctcgg
     2881 cggaaagcgc atccgcaaat tgccgttcta ttgttcgaat caatccctac tattggcgaa
     2941 tgcctttgat cgtttccttg ggtgctctcg gggtcttcat atggggtgca cgatcggcat
     3001 gggccgaagt gggaagccct caacgaccca acattgtctt catcttggcc gatgatctgg
     3061 gttatgggga cctgccatgt tatggccgag atgatctttc cactcctgcc atcgatcggc
     3121 ttgcgagaga aggtgtccgc ttcacgcagg cttacgccaa cggacctgag tgcacgccca
     3181 caagggcggc tttcctgacg gggagatatc agcagcgcat cggcggactt gagtgtgcta
     3241 ttgggattgg cgacgtcggg cgttatgacg atgctatgcg actagctttg gctgggaagc
     3301 tagggttacc cactgaggaa acaaccatcg tctccctatt gcaaaatgcc ggatatgata
     3361 cggttttgtt cggaaaatgg catctcgggt atgcgagcaa gttt
//
LOCUS       DAOPTR010000353         3402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22207_length_3402_cov_3.442187, whole genome shotgun sequence.
ACCESSION   DAOPTR010000353 DAOPTR010000000
VERSION     DAOPTR010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22207_length_3402_cov_3.442187"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1959
                     /locus_tag="PKI05_07755"
     CDS             <1..1959
                     /locus_tag="PKI05_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52137.1"
                     /translation="RCFNPAGLKLRTYYNNWEWGHRYVLNLYPGASYTRYYRSLGTTR
                     EYYVPNQGKDPESVNPRYRIRGNGIWEFRPDLAPAGFSDAVHESINVIATPEKIVRPL
                     EAGQVGHVIFKVQGANIITSQIIRAKGFTKSSADRIEVSISTTVGRTWQKVCEIQSVG
                     EFQIDCHLVDEVSGSYEVLVKFSLLAAENVEHVGISDIQIETRTMLNSKTQPLLRLGQ
                     NTVFVKLGEPTDTIVVWPDLQGENYRPYVVAESNISTEPTHKGWHAVMFAQKPNEEAF
                     TIYRIAAPRPLTRVIYGGRFYNRVPKGSISLSHSWDGGTTWELDWQLTDTSQPWDVVH
                     YVTLTNIPPGTRDVLLKYSMQSPQVGPTACGIYSVRMEVQHQNNGPRFRPLDVTFAWD
                     EIQADRTRVRRTHRQRIDSVPTRYTINVGGVDHPVVESLRIALAEDSPGATYGYSDES
                     PGLVNRVLDVWQECGRNLLQGKPYTLSAEPTGAWGADDPRRQKLTDGVVGSNYAGGTA
                     MKYAAGFDEKSSPVDITVDMGELKTITALGIHLTAGWPWWDALKGEVRDEIEAFTSRD
                     GQTFESQGTFQLNLLRRDIPINYMLPDDETLRAWNYILPLQSPVTAQFVRFRVTARRS
                     LGITEVQAFDRLERRDFWMRILLPDEKP"
     gene            complement(2027..3283)
                     /locus_tag="PKI05_07760"
     CDS             complement(2027..3283)
                     /locus_tag="PKI05_07760"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOA52138.1"
                     /translation="MMHKRVGCILLAITTALVVLFLNANQNGFALSEETTPLSQQYVI
                     LSEEELLDRIYGGWVGMLIGGLEGLPHEFKYVEKPSPTLPEFTFLANGARSDDDNDIE
                     WLHLWFMHQKGTLKIPYDELPAIWKANMNQGVWVANKRARELMDVGIVPPLTGSIEHN
                     PHAWYNLAGQFCVESYGLIAPGMPQTAADIGLHYARISVSQEPLQATQFWTTLISRAF
                     VLSLSTRELLEDALSAVDQRSDMAHAIRDAIAAHQKYPTDWKAARNEIHQRWYKARGW
                     NANSVIVNGAAVCLALLYGDGDFYQTLRYAMAIGYDADCNAATAGTVVGVRWGFRRIS
                     EHPQFRMPDRYQNRTRPQLPQEMRVSEQANILLQLARKVIVEQGGEVMQKDGQIYYRI
                     RQQEPKLLEPLPEVVKRPTPKDKAPS"
BASE COUNT          781 a          876 c          931 g          814 t
ORIGIN      
        1 cgctgtttta atcccgcggg gcttaagctc cgcacctatt acaataactg ggaatgggga
       61 caccggtatg tcctcaacct gtatcctggt gctagctata cgcggtacta tcggagcctc
      121 gggaccacgc gggagtacta tgttcctaat caaggaaagg accccgaatc tgttaacccg
      181 cgctatcgaa ttcgtggaaa cggcatctgg gaatttcgac cagacctggc acccgcaggc
      241 ttttcggacg cggttcatga atctattaac gtgattgcta cgcctgaaaa gattgttcgt
      301 ccgcttgagg ctggccaggt tggtcacgtg atcttcaagg tccaaggggc caacatcatc
      361 acttctcaga tcattcgggc aaagggcttc acaaagagct cagcggacag aatcgaagtt
      421 tctatcagta ccacagtagg gcgaacttgg caaaaagtgt gtgaaattca gtcggtcggt
      481 gaatttcaga ttgattgtca tctcgtggat gaggtatctg gcagttacga ggtgttggtc
      541 aagttttcct tgttggccgc ggaaaacgtc gagcatgttg gcatctccga catccagatc
      601 gagacccgaa ccatgctcaa cagcaagacc cagcccctat tacggttggg gcagaatacc
      661 gtatttgtta aattggggga gccgacagat acgatcgtcg tatggcctga tttgcagggt
      721 gagaattatc gtccgtacgt cgtcgccgaa agcaacatct ccaccgagcc gacccacaag
      781 gggtggcatg ctgtgatgtt tgcgcagaag ccgaatgagg aagcctttac aatttaccgc
      841 attgccgctc ctcgcccatt aacccgcgtg atttatggcg gccggtttta taatcgcgtt
      901 cccaaaggca gcatcagtct ttcgcactct tgggacggag gaaccacctg ggagttggat
      961 tggcaactga cggacacatc gcaaccttgg gacgtcgttc actatgtaac cttgactaac
     1021 attccaccgg gcacacggga tgtgttgcta aaatactcaa tgcaaagccc ccaagtcggc
     1081 cccacggcgt gtgggattta tagcgttcgc atggaagttc agcatcaaaa caacggacca
     1141 cgttttcgac cactcgatgt gacattcgct tgggacgaaa tacaagccga tcgaacgcgt
     1201 gtgcggcgaa ctcatcgtca gcggatagac agcgtaccca cgcgctacac gatcaatgtc
     1261 ggtggtgtgg atcatcccgt ggtggagagc cttcgtatcg ccttggcgga ggattctcct
     1321 ggggcaacat acggttactc ggatgagagc cccggtttgg taaaccgcgt acttgacgtg
     1381 tggcaagaat gtggtcgcaa tttactacaa ggcaagccgt acacgttaag tgcggaacca
     1441 acgggtgctt ggggtgccga tgatccccgg cggcagaaac taaccgatgg cgttgttggc
     1501 tcaaactatg caggcggcac ggccatgaag tatgcggcgg gtttcgacga gaagtcttcg
     1561 cccgttgaca ttacggttga catgggagaa ttaaaaacaa ttaccgcgtt ggggattcac
     1621 ctcacggctg gctggccgtg gtgggatgcg cttaaagggg aagtccgcga tgaaatcgag
     1681 gcttttacct cgcgcgatgg tcagactttc gaatcgcagg ggaccttcca attgaatctg
     1741 cttcgtcggg atatcccgat caactacatg ctgcccgacg atgaaactct ccgggcttgg
     1801 aattatattc taccgcttca atcacccgtc accgcccagt ttgtgcggtt tcgtgtcaca
     1861 gcacgacgga gcttaggcat cacggaagtg caggcctttg atcgcttgga acgccgtgac
     1921 ttttggatgc gaattctttt gccggacgaa aagccctaat tcacccgccg aaggtcttgg
     1981 gcctgtcttt tccggttgct cacaaagttc attgagtgcc tgtatcctat gacggcgcct
     2041 tatccttggg ggtcggcctt ttaacaactt ctggaagcgg ctcaagaagc ttgggctctt
     2101 gctgacggat gcgatagtaa atttggccgt ctttttgcat cacttcacca ccttgctcaa
     2161 cgattacctt cctggcaagt tgcaagagga tgtttgcctg ttcgctgacg cgcatttctt
     2221 gcgggagctg aggccgggtt cggttctgat accgatcggg catgcggaac tgaggatgct
     2281 ctgaaatacg gcggaatccc cagcggaccc ccacgacggt tcccgcggta gctgcattgc
     2341 aatctgcatc gtacccgata gccatggcgt aacgcagggt ttgatagaag tcaccatctc
     2401 catagagaag tgcaaggcat acggctgcgc cgttcacaat gacagaattg gcattccaac
     2461 cacgcgcttt ataccaccgc tgatggatct cgttgcgtgc tgctttccaa tcggtagggt
     2521 acttttgatg ggcggcaatg gcatcccgaa tcgcgtgggc catgtcgctc cgctgatcga
     2581 cggccgataa ggcgtcctcc agcaattccc tggtggataa cgacaagacg aatgcgcgac
     2641 ttattagtgt cgtccaaaat tgggtggcct gaagtggctc ttgtgacacg gaaatccggg
     2701 catagtgtaa cccaatgtca gcggccgtct ggggcattcc gggagcgatc aagccgtatg
     2761 attccacaca gaactgaccc gccaagttat accatgcatg cgggttatgc tcgatcgacc
     2821 cggtcaaggg tgggacgatt cctacgtcca taagctcccg cgcccttttg ttagccaccc
     2881 aaactccctg gttcatattg gccttccaaa ttgccgggag ttcgtcatag ggaatcttta
     2941 gagtcccttt ttggtgcatg aaccagaggt ggagccattc gatatcgttg tcatcatccg
     3001 agcgagcccc attggcgaga aaagtgaact cgggaagcgt cgggctgggt ttttctacat
     3061 acttgaactc gtgaggaagg ccttctaggc ctccgataag catacctacc catccgccgt
     3121 aaatgcggtc gagaagctct tcttccgaca aaatcacata ctgttgcgaa agcggtgtag
     3181 tctcttcaga caaggcgaac ccgttttggt tggcgttaag gaataacacc accagggcag
     3241 tagttatcgc gagcaagatg caaccgactc gcttgtgcat catggatacc tccttcgaat
     3301 ttctcatagg gcatgcgcga ctagcaagtg ttaggatagg gaaatgaatg ggtattgtca
     3361 aagtgctagc actcggtgat gaggcgggat catcaccata tc
//
LOCUS       DAOPTR010000354         3400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22219_length_3400_cov_3.310314, whole genome shotgun sequence.
ACCESSION   DAOPTR010000354 DAOPTR010000000
VERSION     DAOPTR010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22219_length_3400_cov_3.310314"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1421
                     /locus_tag="PKI05_07765"
     CDS             <1..1421
                     /locus_tag="PKI05_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA52139.1"
                     /translation="GANEKVGIAIIGVRGRGGEHIDAFLSDPRVQILYIVDADEKVGQ
                     GRVEQIYKAKGFRPKFIRDFRQALDDAAVDAISTATPNHWHALCGVWAMQAGKDAYIE
                     KPICHNIAEGAALVAAAKKYKRICQVGTQCRSNPAIRNAVEFLRSGGIGEVKFARGLC
                     YKRRKSIGPKGDYPIPPEVDFNLWSGPAPFTTPRCTRPNFHYDWHWQRLYGNGDLGNQ
                     GPHQTDIARWGLGINSHPISVISYGGRLGYQAERNDDNYVDAGDTANTEVSIYDYGDK
                     CIVFETRGLSVDNSADEEINVLFESKTGNKIGVIFYGTDGYLVQQTYTRCVVFDKNLK
                     PVKTFGGSGNHFANFIDACLRRDPKILNSDAWEGHLSAALSHLGNISYYLGENNKVSP
                     EEAKRILAGVKSLDDNIATLERTLRHLQDNGVDLKKYPISMGPLLKFDPEREVFPDSP
                     EATALVAREYREPFVCPKADKV"
     gene            1540..2466
                     /locus_tag="PKI05_07770"
     CDS             1540..2466
                     /locus_tag="PKI05_07770"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOA52140.1"
                     /translation="MRTRWVIAGTLLLIAGCVGVFIFLVSTPGLRLMSSKNPPRSTEA
                     FAVEKSAFGGNGSSSVSKDDPVNPAEQSEEKIVSGPCRPVFLEEPEAFRLARQRMVQF
                     QLQNRDIIDADVLYVMGRIPRHEFVPENVIHEAYDDHPLPIGHGQTISQPYIVALMTQ
                     LAKPRKESRALDIGTGSGYQAAVLAELCREVYSIEIIEPLAKSAAERLKRLGYTNVVV
                     RAGDGYRGWPEKAPFDVIIVAAAPDHVPKPLVDQLALGGRLVIPVGRGQQDLLVIEKQ
                     PDGSIRQTSVAPVLFVPMTGEAQQLGKSSSNP"
     gene            2638..>3400
                     /locus_tag="PKI05_07775"
     CDS             2638..>3400
                     /locus_tag="PKI05_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52141.1"
                     /translation="MWRSQQLVLNFFLAVVLVVIGHFNSGSLVWGQNIVNGGFEQDAD
                     NDGLPDEWVTAGRSSIRQTLTRVLEPGRGYVACLKCTHFEGGFPDSHVMLAQLNRVGV
                     TRGQWYRLRLWARAEDLEFGALSISLVNRKIWQDVGLRETFSPRADWKPYEFHFRATR
                     DLAPEDSRFQIYFQGTGTLFLDDIVLEPVSGFRPQRLPQLPTKWLKNLLVDSSFECGA
                     SDWGTVALGITSWAGNLFHRVGTWDSTKAYHGTHSW"
BASE COUNT          750 a          883 c          978 g          789 t
ORIGIN      
        1 aaggtgccaa cgagaaagtt ggcatcgcaa ttattggtgt gcgtggtcgc gggggagagc
       61 atattgacgc cttcctcagt gacccccgcg tccaaatcct ctatattgtg gatgccgatg
      121 aaaaagtcgg ccaagggcgg gttgagcaga tttataaggc taagggtttc cggcccaagt
      181 tcattcggga tttccgacag gcactggacg atgccgccgt ggatgccatc agtacggcaa
      241 ctcctaacca ttggcacgcc ttgtgtggtg tttgggccat gcaggcaggc aaagatgcct
      301 atattgagaa gcctatttgc cacaacatcg ctgaaggggc cgccctcgtt gccgcggcca
      361 agaagtacaa gcgaatctgc caagtgggaa cgcagtgccg ctcgaatccc gcgatccgca
      421 acgcggtgga gttcctccgg tctggcggga tcggtgaggt aaagttcgcc cgcggccttt
      481 gctacaaacg gcggaagtcg atcggtccga agggtgatta tccgattccg cccgaggtcg
      541 atttcaattt gtggagcggc ccggcacctt tcaccacgcc gcgatgcacg cggcccaact
      601 tccactatga ttggcactgg cagcgcttat acgggaatgg tgatttgggc aatcaggggc
      661 cgcatcaaac ggatatcgcg cgatggggtt tgggcatcaa ttcccatccc attagtgtga
      721 tctcctacgg tggtcggttg ggttatcaag ccgaacgaaa cgatgacaac tatgtcgatg
      781 ccggtgatac cgcaaatacg gaggtgtcta tctacgatta cggcgataag tgcatcgtct
      841 ttgaaacgcg tgggttgtcc gtggacaatt cggccgatga agagatcaac gtcctcttcg
      901 agagcaagac tggcaacaag attggcgtca ttttctatgg cacggacggc tatcttgtcc
      961 agcaaacgta cacgcggtgc gtggtattcg acaagaacct aaagccggtc aagacatttg
     1021 gtggctccgg caatcacttc gccaacttta ttgatgcgtg cctccgtcgg gacccgaaga
     1081 tcctcaattc ggatgcctgg gaaggacacc tctccgcagc cctcagtcac ttgggtaaca
     1141 tctcttacta tttgggagag aacaacaaag tctcacccga agaggccaaa cgaatcctgg
     1201 ccggtgttaa aagtctcgat gacaacatcg cgaccttaga acggacgctc cgtcatctcc
     1261 aggataacgg cgtcgatctg aagaagtacc cgatctcgat gggaccgttg ctcaagttcg
     1321 atcccgagcg ggaagtcttc ccagattcgc cagaggctac ggccttggtc gctcgggagt
     1381 atcgagagcc ctttgtctgc cccaaagccg acaaggtgta acacaatcca ctttgttcgt
     1441 ggggtgacaa taggcgataa aggctgtgat attgtcgcca tcgcgagagg atttggaccg
     1501 gtttccgcaa gcttgggcca aacaatcggg aggtgagcta tgcggacgcg gtgggttatt
     1561 gccggaacgt tgctcctcat tgcgggctgt gtaggtgttt tcattttttt ggtgagcacc
     1621 cccggcttga ggttgatgtc ttccaaaaat ccccctcgat cgaccgaggc atttgccgtg
     1681 gaaaagtcgg cgttcggggg aaacggtagc agttcggttt cgaaggatga tcccgtgaac
     1741 cctgccgaac agagtgagga aaagatcgta agcggtccgt gtcggccggt atttctagag
     1801 gaaccggagg cctttcgctt ggcccgacag cggatggttc aattccaact tcagaaccgt
     1861 gacatcatcg acgccgatgt tttgtacgtg atgggacgga ttccacgtca cgaattcgtt
     1921 ccggaaaacg tgatccacga ggcgtatgac gaccatcctt tgccgatcgg gcatggccag
     1981 acgatatcac agccgtacat cgtcgccctt atgacgcagt tggccaaacc gcggaaagag
     2041 agccgtgcct tagatatcgg tacgggatcg ggataccaag ccgcggttct cgccgaattg
     2101 tgccgcgagg tgtacagcat cgagatcatc gaacctttgg ccaaatccgc tgcggaacga
     2161 ctcaagcggt tgggttacac caatgtcgtt gtccgggcgg gcgacggcta tcgcggctgg
     2221 ccggaaaagg ctccttttga cgtaatcatc gtagcagccg ccccggatca tgtccccaaa
     2281 cccttggtgg atcaactcgc cttgggcgga cgattggtca tacctgttgg ccgcggtcag
     2341 caagatctgc tggtaattga gaagcaacct gacggttcca ttcgtcaaac cagtgttgca
     2401 cccgtattat ttgtaccgat gacgggcgag gcacagcagc tgggcaaaag ttcaagcaat
     2461 ccgtaaggtg tcctcatttt gtctggcgaa gattaagcgg ctctcatctc gttagtctct
     2521 gcgagcaaag tgcgccgcag ccatcaccga cactctccct ctgtataggc ccctctgtac
     2581 aatagggggc aggtaccaaa ccactcgagc ccgccagtaa tcatagaggt atgtcccatg
     2641 tggaggtctc agcagctcgt acttaacttt ttcctcgctg ttgtcttagt cgttattggg
     2701 catttcaact ctggctcact ggtttggggg caaaacattg tcaatggcgg atttgaacaa
     2761 gatgcggata atgatggtct gccggatgaa tgggtaaccg ccggtcggag ttcaatccgg
     2821 caaacgttga cgcgggttct cgagccagga aggggctacg ttgcctgttt gaagtgtacg
     2881 cattttgagg gcggttttcc tgatagccat gtgatgttgg cacagcttaa tcgggttggt
     2941 gtcacgcgcg gccagtggta ccgtctgcga ctttgggccc gcgctgagga cctcgagttc
     3001 ggggcccttt cgatttcact tgttaatcgg aagatttggc aagacgtagg ccttcgcgag
     3061 acgttttcgc cccgagccga ctggaaaccg tacgaattcc atttccgggc tactcgtgat
     3121 ctcgccccgg aagatagtcg ttttcaaatc tattttcagg gaacaggaac tctatttctg
     3181 gatgatatcg tcctcgagcc agtctccggg tttcgtcccc aaagactccc tcaattaccg
     3241 acgaaatggc taaagaatct tttggttgac agcagcttcg agtgtggcgc ctcagactgg
     3301 gggacggtag ccctggggat aacatcctgg gcaggaaacc tattccaccg cgtggggacg
     3361 tgggactcga caaaggctta ccatggcact catagttggc
//
LOCUS       DAOPTR010000355         3397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22259_length_3397_cov_4.076302, whole genome shotgun sequence.
ACCESSION   DAOPTR010000355 DAOPTR010000000
VERSION     DAOPTR010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22259_length_3397_cov_4.076302"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PKI05_07780"
     CDS             complement(<1..489)
                     /locus_tag="PKI05_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOA52142.1"
                     /translation="MPLGLDGGVYIGIAYSMGYPDQDPLAWIEISLKELEDAGLRRQC
                     RTRECSQGRMIRFGERLLLNFGSNDYLGLAADTRLIQAVSEAIHREGWGSGASPLICG
                     RSISHAALESALARFEGTEAALVFPTGFAANSGTIAALVGPGDAVYSDKKNHASLLDG
                     CRL"
     gene            complement(503..847)
                     /locus_tag="PKI05_07785"
     CDS             complement(503..847)
                     /locus_tag="PKI05_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOA52143.1"
                     /translation="MSPKTVFKKIIDREIPAEIVYEDQECLAFKDINPQAPIHVIVIP
                     KKEIPNLEELREEDEALIGHLFIAMQRIAQQMGISTGYRVVLNNGPDAGQEVMHMHFH
                     LLAGRKFDWPPG"
     gene            complement(918..1202)
                     /locus_tag="PKI05_07790"
     CDS             complement(918..1202)
                     /locus_tag="PKI05_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HOA52144.1"
                     /translation="MIDPQLLSLLACPETRQPLSLGDSALLSQINQAIAVGKLRNRAN
                     CQVTQPLEAILVRQDGKVGYPVWDGIPTLLIDEGIPLGQLEMGGVENTSA"
     gene            1479..1940
                     /locus_tag="PKI05_07795"
     CDS             1479..1940
                     /locus_tag="PKI05_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOA52145.1"
                     /translation="MCGSELQAKPKEKATGQERPGRILLVDDDRAAIEPLHIQLQIEG
                     FEVLLTETGQQGLDLARSWHPDVIVLDLCLPDVHGLEICQALMTTSATADIPIIILSG
                     MEGRDLPRRCRAAGGMYYVAKPYDPNVLLLLISQAAADHRLWHDAGSQNSE"
     gene            2395..>3397
                     /locus_tag="PKI05_07800"
     CDS             2395..>3397
                     /locus_tag="PKI05_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA52146.1"
                     /translation="MALRSSRREFLQASAVAGVGFWVAGGVRAEESKSPNEQIQFACI
                     GMGGKGQSDSADAGNHGVVVAICDVDDRTLEGASKRFPNAKKYFDFRKMLEEMGDKID
                     AVTVSTPDHTHAAAALMAMRMGKHCFCQKPLTRCIYEARLMGQVAREKKVQTQMGNQG
                     TANPALRRAAALLKKNILGTVKEVHVWTNRPIWPQGGERAAEQPVPPYLHWEEWIGPA
                     KFRPYAPGYHPFSWRGWWDFGTGALGDMACHTLNMPYAGLDLRNPVSVQAETSGHNRD
                     SFPKWSIITYEFAANDWRPALTLKWYDGGKLPPKELFTEFEDPKAPTASGALIIGEKG
                     "
BASE COUNT          781 a          956 c          884 g          776 t
ORIGIN      
        1 gaggcggcag ccatcgagca aacttgcgtg gtttttcttg tcgctgtaaa cggcatcacc
       61 tggccctacc aatgcggcaa ttgtgcctga attggccgca aaacccgtcg ggaaaacaag
      121 agccgcttcc gtaccctcaa accgggccag ggcggattct aacgccgcgt ggcttatgga
      181 acggccacaa atcagcgggc tggcaccgct cccccaccct tcgcgatgaa tcgcttcgga
      241 gaccgcctga atgagccgcg tatctgcagc tagccccaag taatcgttcg atccaaaatt
      301 gaggagcagc cgttcgccga atcgaatcat tcggccttgg ctgcattcac gcgtccgaca
      361 ttgccgacgc aaaccagcat cttcaagctc tttcaagctg atttcaatcc aagcgagtgg
      421 gtcttgatca gggtaaccca tggaatatgc aataccgata tagaccccac cgtcaagccc
      481 caatggcatc ccgcaagacc aatcacccag gtggccagtc gaacttgcgc ccagccagca
      541 ggtgaaaatg catgtgcatc acctcctgcc cggcatctgg cccattatta aggacgaccc
      601 gataccccgt gctgatcccc atttgttggg caattcgttg catcgcgatg aagaggtgtc
      661 ctatcagtgc ttcatcctct tcccttaatt cttccaagtt gggaatctcc tttttaggga
      721 tgacaatgac atgaatcggt gcctggggat taatatcttt gaaggccaaa cactcctggt
      781 cctcataaac gatttctgcc ggaatctcgc gatcgatgat cttcttgaag actgttttcg
      841 gcgacatggc acactctcca agcgtctaaa acccagcctc cctgaactgc caacaccaga
      901 accactgttc gaagcactta cgcggacgta ttctcgacac cacccatttc gagctgaccg
      961 agtggaattc cttcgtcgat gagcaacgtc ggaatcccat cccagacagg ataacccact
     1021 ttcccatctt gcctaaccag aatcgcttcc aagggctgcg tcacctggca attggcacga
     1081 ttacgcaatt tgcctaccgc aatggcttga ttgatttgac taagaagagc gctatctccc
     1141 aaagaaaggg gctgcctagt ttcaggacag gccaggagtg aaagcaattg tgggtctatc
     1201 atctgtcgat cgttggcttg aagttcctga cagctgtcaa aagacgcaac gtgaacaacc
     1261 ggacatcgat tgtctcaata gcccaagaaa atggcaacgt tctacgatta accgtcgtcc
     1321 ttccggaata ttcatcatat accacattat ctcctgagaa cacgagggcg ctattttgca
     1381 ttttcttttt ttccgtttgc caaggccaat ttcttgacct atgctctcaa tgctaagatg
     1441 tagcttgctt gccggtattt atggaaattt cgccacaaat gtgcggatca gaacttcaag
     1501 ccaaaccgaa agaaaaggca accgggcagg aacgacctgg tcggattctg ctggtggatg
     1561 atgatcgagc cgcaattgag ccgcttcata tccaactgca gattgaaggg tttgaagtcc
     1621 tgttaaccga gaccggccag caagggctgg atctcgcccg gtcgtggcac cccgacgtga
     1681 ttgtcctcga cctttgtctg cccgacgtgc atggtctaga aatctgtcag gcattaatga
     1741 cgacctctgc tacggccgac atccccatca ttattctgag cggtatggaa ggtagagacc
     1801 taccccgccg gtgccgcgcc gcaggtggaa tgtactatgt ggcgaagcca tatgacccca
     1861 acgtccttct cttactgatc agccaggccg cggctgacca ccgactgtgg catgacgcag
     1921 gcagtcaaaa ctctgagtga tttgcattct cataagttcc tccgccacgg cgagtaaccc
     1981 ttaatcactc gtttcgtcgc cttccatcac catatccctt gagaaacgcg catcgctgcg
     2041 tccctcaatc acacgagcca ccagtacaaa ctagcgatgc ccgtttctcc taggggttaa
     2101 aaattcagag atatgatcaa gagacacatc cgtcctacaa tggggggtca agatgttgcg
     2161 gacatcgtac cgatatcatc gccttatcag tgattgttag gtgccgctcg taattcttcg
     2221 gcgttcacat tcgcagcgcg ggcaggtgtc ccctcgaagt catttaacct ccctggttcc
     2281 gcggaacttg caggagcctt ttgaatgagt tatcctatcg cgtaattggt caagacttgg
     2341 cagaccaggt cttccggtcc atacaaacat acgtttactt gaggaggatt cgttatggca
     2401 ttgcgttcaa gtcgtcgtga gttcttgcaa gcgtcagccg ttgccggggt cgggttctgg
     2461 gtcgccggtg gtgtccgcgc tgaggagagc aagtcgccga acgagcagat tcagtttgcc
     2521 tgcattggta tgggcggtaa aggccagagt gattcggcgg acgctggcaa tcatggggtg
     2581 gtcgttgcca tttgcgacgt cgatgatcgg acgcttgagg gagcatccaa gcgatttccg
     2641 aacgccaaga agtattttga ctttcggaag atgctcgagg aaatgggcga taagatcgac
     2701 gcggtgaccg tcagcacgcc agaccatacg cacgcggcag cggccctcat ggccatgcgg
     2761 atgggcaagc actgcttttg ccagaagccc ttgacccgtt gcatctacga agcccgtttg
     2821 atgggtcaag tcgcccgtga aaagaaggtg cagacccaga tgggcaacca agggacggcg
     2881 aatccggctt tgcggcgggc cgcagccctc ttgaagaaaa acattctggg aaccgtcaaa
     2941 gaggttcatg tctggaccaa ccggcccatt tggccgcaag gtggggaacg cgctgcggaa
     3001 cagcccgttc caccttacct ccactgggaa gagtggattg gacccgcgaa gttccgtccc
     3061 tatgctccgg gttatcaccc gttctcgtgg cgcggttggt gggacttcgg caccggtgcg
     3121 ttgggcgaca tggcctgcca cacgctgaac atgccgtatg ccggtttgga tctccgcaat
     3181 ccggtgtcgg tccaggccga aacctcgggc cataatcggg atagcttccc caagtggtcg
     3241 atcatcacct acgaatttgc ggccaacgat tggcgtcccg ctttgaccct gaaatggtat
     3301 gacggtggca agttgccgcc caaggagctg ttcactgagt tcgaagaccc caaggccccg
     3361 accgcaagcg gtgccctgat catcggtgag aagggta
//
LOCUS       DAOPTR010000356         3396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22273_length_3396_cov_3.410057, whole genome shotgun sequence.
ACCESSION   DAOPTR010000356 DAOPTR010000000
VERSION     DAOPTR010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22273_length_3396_cov_3.410057"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="PKI05_07805"
     CDS             <1..1050
                     /locus_tag="PKI05_07805"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOA52147.1"
                     /translation="GDETSNRMQPLELVATPGASTIEQVSRFLQCRPQDLIKTLIYLA
                     DGQPVAVLIRGDHEANENKIRRAFQAKEIVLADPKTIERVTGAPVGFAGPIGLKENIP
                     IIADYDIQYVRNGVTGANQADAHYRGVNLGRDFQVTRFADLRCAVDNDPCPRCSGILR
                     LRPAIEIGHVFKLGVKYSEALNARFLDADEQLKPIIMGCYGIGVNRILAALIETTHDE
                     NGIVWPVALAPYEVVIVPVNVTDEQTRQVATALHDELSKEEIDVILDDRDVRAGVKFK
                     DADLIGFPLRIVVGERGLAQNSVEVKWRWDKNSQMLPLDSVVREVAQWIREERKTGQR
                     FKSAVYGNRSGSPTR"
     gene            complement(1089..1880)
                     /gene="dapB"
                     /locus_tag="PKI05_07810"
     CDS             complement(1089..1880)
                     /gene="dapB"
                     /locus_tag="PKI05_07810"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOA52148.1"
                     /translation="MEKIRLAISGAAGRMGQRVIALAMNEPDFEIVAAIDRADHPRLG
                     QDAGVVAGVSPLGVSLKSSLETPADVMIDFSHPSATDKVIELCRLHRMALVFATTGIS
                     PEQEKALVELSRQVPIVRAPNMSLAVNLAMKLCQIAAHSLREHDADVEIIERHHRFKE
                     DSPSGTALQFGKTIAEIMGQTIHRHGREGRPGRRPHEEIGYHAVRVGDNPGEHTIIFG
                     MLGETLEITVRASNRDAYAKGALAAARFIVKQPAGLYGMQQVLQL"
     gene            complement(1945..3345)
                     /gene="thrC"
                     /locus_tag="PKI05_07815"
     CDS             complement(1945..3345)
                     /gene="thrC"
                     /locus_tag="PKI05_07815"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOA52149.1"
                     /translation="MNPTDYAFQRCIFPDCGATFGVEETHIGCTRCGGLLEVAYEWDR
                     LPVPSSLREFERLWGQRHDPFAFSGVWRFHSLLPFSPREKCVTVGEGQTLLHSSDRVA
                     RYVGLNPGRLFLQYEGMNPSGSFKDNGMAAAFTHARLIGATRAACASTGNTSASLALY
                     CAVTGLMKAIIFIGSGKIAYGKLSQALDYGALTVQIAGDFDDAMKRVQEVSRSLGIYL
                     VNSVNPFRLEGQKTIMFRVLEGLRWEVPDWIAVPGGNLGNSSAFGKAFRELKELGLIP
                     RVPRLAVINATGANTLYRLYEQQGVRWNQGRVDKTQVGEFYQVMDQEQRRASTIASAI
                     EINRPVNLMKCLQALETCQGVVREVTDQEILDAKAQVGAGGLGCEPASAASVAGVKRL
                     VAEGVIGRDERVVCVLTGHQLKDPTATVAYHTTDQEMFNKVLGTRGVHRATFANRAVT
                     VANDLNEIIKAIQIYG"
BASE COUNT          780 a         1019 c          897 g          700 t
ORIGIN      
        1 ggggatgaaa cgtccaaccg catgcaacca ctcgagctgg tggcaacccc tggggcaagc
       61 acaattgagc aggtaagtcg cttcttgcaa tgtcggcccc aggacctcat caagacattg
      121 atttacctcg ccgacgggca accggtcgcc gtactcattc ggggcgatca cgaagccaac
      181 gaaaacaaga tccgccgggc ctttcaggcc aaagaaatcg tcctggcgga tcccaaaacc
      241 atcgaacgcg ttacaggggc cccggtgggc tttgccggac caatcggcct gaaagagaat
      301 attccgatta ttgccgatta cgacattcaa tatgtccgca acggcgtgac gggggccaac
      361 caggccgacg ctcactaccg tggggtcaat ctagggcggg actttcaagt gacccggttc
      421 gcggatttac gatgcgccgt ggataacgat ccgtgtccgc ggtgtagtgg cattctgcga
      481 ctgcggcctg ccatcgagat aggccacgtc ttcaagttgg gcgtgaaata cagcgaggcc
      541 cttaacgccc ggtttctcga tgcggacgaa caactcaaac cgattatcat gggctgctac
      601 gggatcggag tgaatcggat tcttgcggcc ttgatcgaga ccacccacga cgagaatggc
      661 atcgtctggc cggtggcgtt ggccccctac gaggtcgtca ttgtccccgt gaacgtgacc
      721 gacgaacaga cccgacaggt tgcaaccgcg ctgcacgacg agctatccaa agaggaaatt
      781 gatgtcatcc tggatgatcg cgatgttcgg gctggggtga agtttaagga cgccgacctt
      841 attggtttcc cgctgcggat tgttgttggc gagcgaggtt tggctcaaaa cagcgttgaa
      901 gtcaagtggc ggtgggataa aaacagccaa atgcttcccc tggacagcgt cgttcgcgaa
      961 gttgctcagt ggattcgcga agagcgcaag accggccaac gatttaagtc cgcggtctac
     1021 ggcaatcgaa gtggctcacc gacacggtga attatcttgc gggtaccgcc gcagataacc
     1081 gcgatcaact atagttgcaa gacttgctgc atcccataca ggcccgcagg ctgttttact
     1141 atgaagcggg cagcagcgag ggcacccttg gcgtaggcat cacggttgct ggcgcggacc
     1201 gtaatctcca gcgtttcgcc caacattcca aaaatgattg tgtgttcccc ggggttgtct
     1261 cccacgcgca cggcatgata accgatctcc tcatgcggtc gccgcccggg tcggccctca
     1321 cgtccatgcc ggtgaatcgt ttgccccata atttcggcga tcgtctttcc aaattgcagg
     1381 gccgtaccac ttggcgaatc ctctttaaaa cgatggtgac gctcgatgat ttccacatca
     1441 gcgtcatgct cgcgcaaact atgtgccgca atctgacaga gcttcatcgc aagattcacc
     1501 gcaagactca tgttgggagc acgaacgatg ggcacctgac ggctcaactc taccaaggcc
     1561 ttttcttgct ccgggctgat tcccgtggtg gcaaagacga gcgccattcg atgaagacgg
     1621 cacaactcga tgaccttatc ggtggctgac ggatgcgaga aatcgatcat cacgtcagca
     1681 ggcgtctcca gcgaactttt cagcgaaacc cccaacggag aaaccccggc caccacgcca
     1741 gcgtcttgcc ccagtcgcgg atgatccgcg cggtcaatag cggcaacgat ctcaaaatcc
     1801 ggttcattca tcgccagggc aatgacgcgc tgccccatac gaccggcagc gccggaaatg
     1861 gccaaacgta tcttctccat taggtgtcaa cctccttgtg atcaacaata cgacgcccca
     1921 caaatgacgt tagggatcag tttttcaacc atatatctga atggctttga tgatctcgtt
     1981 cagatcattg gcaaccgtca ccgcccgatt ggcgaatgtg gcccgatgaa caccgcgcgt
     2041 acccaggacc ttgttgaaca tctcctgatc ggtggtatga taggcgacgg tggccgtggg
     2101 atccttcagt tgatgtcccg tgagtacgca cacaacccgc tcatcgcgac cgatgacccc
     2161 ctcggcgacg agccgcttca ccccggcaac gctggccgca ctggccggtt cacatcccaa
     2221 cccacctgca cccacctggg ccttggcatc cagaatctct tgatcggtca cctcgcggac
     2281 gaccccctgg caggtttcaa gggcctggag acatttcatt agattaaccg gtcggttaat
     2341 ttcgatggcg ctggcaatcg ttgacgcccg ccgctgctcc tggtccatta cctggtagaa
     2401 ctccccgacc tgggtcttat cgactcgccc ctggttccac ctgacaccct gctgttcata
     2461 aaggcggtac agcgtgttgg ctcctgtcgc gttaatgacg gccaatcttg ggacgcgcgg
     2521 gatcaacccc aattccttaa gttcccgaaa cgccttacca aaagcactac tattacccaa
     2581 attgccacca gggacggcaa tccagtccgg gacctcccag cgtagtccct ccaggacccg
     2641 gaacatgatt gtcttttgcc cttctaaccg aaacggattc acactgttaa cgaggtaaat
     2701 gcccaaggaa cggctgacct cctggacgcg cttcatcgcg tcatcaaaat ccccggcaat
     2761 ttgaaccgtc agtgccccat aatccagtgc ctgggagagc tttccgtagg caatcttccc
     2821 ggaaccaata aagatgatgg ctttcatcag gccggtgacc gcacaataaa gcgccaaaga
     2881 ggcgctggtg ttcccggtgg aggcacatgc tgcccgcgtg gccccgatta atctggcatg
     2941 ggtaaatgcg gcagccattc cgttgtcctt gaaactcccc gacggattca tcccctcata
     3001 ctgcagaaac agccgtccag ggttgaggcc gacatatcgg gcaacacgat cagaagaatg
     3061 caagagcgtc tggccttctc cgacggtcac gcatttctct ctcggggaaa atggcaaaag
     3121 actgtggaat cgccacacac cgctaaaggc aaacgggtca tgcctctggc cccacagccg
     3181 ttcaaactcg cgtaaagagg acggaactgg cagcctatcc cattcgtagg ctacctcaag
     3241 caacccgcca catcgcgtgc aaccgatatg ggtttcctca acgccaaacg ttgccccgca
     3301 atctgggaaa atacaccgct gaaaggcata atccgtcgga ttgatggcta ggctcctttc
     3361 cgatggctta gcaaaagcaa agaagcgcga tccaga
//
LOCUS       DAOPTR010000357         3393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22290_length_3393_cov_4.269323, whole genome shotgun sequence.
ACCESSION   DAOPTR010000357 DAOPTR010000000
VERSION     DAOPTR010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22290_length_3393_cov_4.269323"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PKI05_07820"
     CDS             complement(<1..727)
                     /locus_tag="PKI05_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52150.1"
                     /translation="MVKKLLIGGGIVVLLGTVLLGRDLVSYVRTSANYVQESVVDAVP
                     MEFQIRRARKMLDDLLPEIRKNMHVIAREEVEVERLERQIAQAREQLEKEKQVILRMK
                     DDLAGGRPTLVYSGREYTAQQVRTDLANRFERFKTNEATLRNLEQIREARLKSLQAAR
                     DKMEGMLAAKRQLEVEVENLQARLQMVQAAQTTSEYQIDDSTLGRVKDLIADLKAKLD
                     VAERLVQSQGFYQGEIPVEDPNQK"
     gene            1291..2763
                     /locus_tag="PKI05_07825"
     CDS             1291..2763
                     /locus_tag="PKI05_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOA52151.1"
                     /translation="MPQHSPKSLTSCQDPSSSQAGNGLADLGKVTQPRRELSLFDSIC
                     IIVGIIIGAGIYETTPKIAGCLPNTQWLLAVWIIGGLLSFLGALCYAELATAFPHSGG
                     DYVFLTRAYGRHLGFLFAWCELWIVRPGSIGMMAFVFARYAREIVPINVAGSQQLSLL
                     TYALGAIGVLTIINILGVRQGKWTQNFLTTLKILSLILIFAVGMFLLSPAKSPLADSP
                     ADKSPPLNLYLAMILVLFTYGGWNEMAYVAAEVKNPTRNILRALVLGTLSVTAIYVLV
                     TLAFVRGLSFAGVQQSSVVASDLLRLAFGEWGGKLIAVIICISTLGVINGQIFTGARV
                     YYAMGRDHSVFGLLGNWDPQRSTPIWSLVVQGIITMGLVAGFGWYQDGFNNLLNFTTP
                     IFWTFFFLVGVSLFVLRAREPHVPRPYRVILFPVVPIAFCCSCCFMIYASVTYAIQNK
                     TYEAIWAIAFLVLGFLVALIFVRSRQEVRESAVTAITPEV"
     gene            3185..>3393
                     /locus_tag="PKI05_07830"
     CDS             3185..>3393
                     /locus_tag="PKI05_07830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOA52152.1"
                     /translation="MSEINRRDFVRTGITAAAGAALGLQPTFTVKAGNPENFDTSKIL
                     NYNPDMEYRRCGKTNLMISAVCLGG"
BASE COUNT          662 a          967 c          907 g          857 t
ORIGIN      
        1 ctttttggtt gggatcctca acgggaattt ctccttgata gaatccctgc gactggacta
       61 accgctccgc gacatccaat ttcgccttga ggtcagcgat caagtctttg actcgcccca
      121 gggtgctatc atcaatttga tactcacttg tcgtctgagc cgcctggacc atttgcaatc
      181 gggcctgaag attctccacc tccacttcga gctgtcgttt ggcggccagc atcccttcca
      241 ttttatcccg agccgcctgg agactcttca gtcttgcctc gcgaatctgt tccagatttc
      301 tcagcgtcgc ttcgttggtt ttgaatcgtt caaaacgatt ggccagatcg gtccgcacct
      361 gctgggcagt gtactcccgt ccggaataca ccagtgtggg cctcccaccg gcgagatcat
      421 ccttcatccg caggatcact tgcttttcct tttccaattg ctcacgggcc tgggcaattt
      481 gccgctccag ccgttccact tccacctctt cccgggcaat gacatgcatg ttcttgcgaa
      541 tttcgggcag aagatcgtca agcatcttgc gggcacgacg aatttgaaac tccatgggca
      601 ccgcatcgac aacactctcc tggacatagt tggccgaggt acgaacgtag ctcaccaagt
      661 cccgaccgag taaaacggtc cccaacagaa cgacaatacc accaccgata agcagcttct
      721 tcaccatgat cgtgaccctc ctttttctcg tgaccctgag gccagggctt aacacacctg
      781 caatcgacag caccaacaag cgagcttttc agctcctgct atccgtatat tcgcacaagc
      841 cgtgagattc tttgggacgt tttcttttta tgccgacatg cccacgggca gaaaaagcca
      901 aaatgcatct caatgagacg tccgcatgtc ggtgctgcta cgccgagagt ggggtcgccg
      961 cccccgggcg tgcgactttt gggggggccg ctagtgcgag gggtaaagag cctatcccac
     1021 aacctccccc cacctgttcg gcggaagagg ggcctggggt gagggtggct cgaatcgctg
     1081 ccggttttgc tcaatcgatc ccgacgttcg cttgccctca ccccaaccct ctcccagagg
     1141 gagagggcga ctttttggga tagactttaa gctcgatcgc ccatcatgca cacgttgcag
     1201 ggatgatcgg gttgattaca atgggcatca tccacgtcct tgatccgatc tatccctatt
     1261 gtccataacc cgaacggagt gcccatcgag atgccgcagc attcgcccaa atcattgact
     1321 tcctgccaag acccctcctc ctcgcaggct ggcaatggct tagccgactt ggggaaagtg
     1381 acccaaccac gccgcgagtt gagtttgttc gattcaatct gcatcatcgt ggggattatc
     1441 atcggggcag gaatttacga aacgacccca aaaatcgcgg gctgcttgcc caatacgcaa
     1501 tggcttctcg ccgtgtggat catcggcggc ctgctctcgt tcctcggggc cttgtgctac
     1561 gcggagctgg caaccgcgtt tccgcattct ggaggggact atgtcttttt gacccgggct
     1621 tatggtcggc atctgggatt tctcttcgcc tggtgcgagt tgtggattgt tcgcccggga
     1681 tccattggca tgatggcgtt tgtcttcgcc cgttatgccc gggaaattgt ccccatcaat
     1741 gtcgccggta gtcagcaact atctttgttg acttatgcgc tgggggccat cggtgtcctg
     1801 acgattatca acatcttagg agttcgccaa gggaaatgga cacaaaactt cttgacaacc
     1861 ctcaagatcc tcagcttaat ccttattttt gcggtcggta tgtttcttct gtccccggcc
     1921 aaaagtcccc tggcggattc gccggcagac aagagcccgc cgttaaatct gtacctggcc
     1981 atgatcttgg tgcttttcac ctatggcggg tggaacgaaa tggcttatgt ggctgcagag
     2041 gtcaagaatc ccacacggaa tatcttgcgg gcattggttt tgggcacgct cagcgtcacc
     2101 gctatctatg ttctggttac ccttgccttt gtgcggggcc tgagtttcgc gggcgttcag
     2161 cagtcaagcg ttgtggctag cgatctccta cgactggcat ttggcgaatg gggagggaaa
     2221 ctgatcgcgg tcatcatctg catttcgact ttgggagtca ttaacggaca gattttcaca
     2281 ggggcgcggg tttactacgc catgggccgc gaccacagcg tctttggcct gctcggcaac
     2341 tgggacccgc agcggagcac gcccatttgg tctctcgtcg ttcaggggat catcaccatg
     2401 ggccttgtcg cgggtttcgg ctggtatcag gacggcttca ataacctgct caattttacc
     2461 acgccgatct tttggacgtt tttcttccta gtgggcgtgt cgctctttgt cctccgcgcc
     2521 cgtgaacccc acgtcccccg gccgtatcgc gtgatcctct tcccggtggt tccgattgcc
     2581 ttctgttgtt cgtgctgctt tatgatctat gcgagtgtga catacgccat ccagaacaag
     2641 acttacgagg ccatttgggc gatcgccttt ttggtgctcg gctttctggt agccctgatc
     2701 tttgtacgat cgcgccagga ggtgcgcgaa agtgctgtta cggctatcac tcccgaggtg
     2761 taaatgccac gggcgtacgg tcgggttcac ggcggagaag cgtcagttgt ctcagcccat
     2821 attctcccct cgcccgttcg gcgggagagg ggtcagggat gatggtacct cgaatcgttg
     2881 ccggttttct cgattaattt cgacgttcgc ttgccctcac cccaaccctc tcccagaggg
     2941 agaaggggac tttttgggat aggctcttaa aaaaataacg gcccaatccg cgctaaggca
     3001 taactatcgc tcataaccga tctagaaaat ccaacgtgcc gtcgatggcg tgaagtgaag
     3061 ttctgttgcc aagcgggttg cggcgcatca taatgatgac cagttgcgga tgctctggtg
     3121 ccgaggataa cttggttggg agagcgtggt ttcgcggaaa tccttttttc ggggagacaa
     3181 agccatgtca gagatcaatc ggcgggattt tgttcggaca gggatcacgg ccgcggcggg
     3241 agcggccctc ggccttcagc cgactttcac agttaaggca ggcaaccctg agaacttcga
     3301 cacaagcaag atcctcaatt acaacccgga catggagtat cgccgttgtg gcaagaccaa
     3361 cttgatgatc tcggcggttt gtctcggtgg gca
//
LOCUS       DAOPTR010000358         3382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22386_length_3382_cov_3.178239, whole genome shotgun sequence.
ACCESSION   DAOPTR010000358 DAOPTR010000000
VERSION     DAOPTR010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22386_length_3382_cov_3.178239"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            476..2269
                     /locus_tag="PKI05_07835"
     CDS             476..2269
                     /locus_tag="PKI05_07835"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOA52153.1"
                     /translation="MELPKRPKDDRFTVPGKYRELEQSVRQKAASQDIPALIVYAFDH
                     RTSLGPFVMVQNWMIPGAPRALASAMYAAGFHKLRVVMQQWTPNVRPSQARIDGQVPQ
                     LLLISSMQIHSARAYELIRDAWTLGDERPLIIAGGAKAIYEPWDFFGLSEDGSVGADV
                     VCTGEEYVLMEFLDRLLDYKLPGEHIRAAFERAREQGVFNDIPGLVFRPDPPRGHPEY
                     LVDTGVQRLVQDLDEFPLPFDALSLFEPPHKRTTLSPEPIPVDQLGKYAKVLAVVTTH
                     GCKFRCPYCPIPAYNQESFRYRSPERIVEEFAGIHSRTGIRYFFGTDDNFFNNRQTVE
                     EVFSAMAKATVNNKPFRDAIIFATEGTEFDVHKNIDLIPLGREAGLRSIWFGIEDLTA
                     DLVKKGQSADKTRVVFRELIKHGIAPMPMMMHHDNQPLWSWGSLRGLINQVGFLRKVG
                     AITCQITLLTPSVGSKGFEKPFEDGIVLKKVAGKAVEDYHYDGNRAIATAHPSPLRRQ
                     LNVLAGYLAFYNPLNLLRALPRLDPLWKERIFTQIQGMIGVAKSIWNAREWFYDLAVG
                     PIEYHTAPPQPKYPLVTPVVRIQESRVPAMG"
     gene            2279..2995
                     /locus_tag="PKI05_07840"
     CDS             2279..2995
                     /locus_tag="PKI05_07840"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOA52154.1"
                     /translation="MRRDGLPGYALAVLLVVLMTVPTAAALGYFVLLESAPSLVQQSA
                     YVAGKVVMTLPAIWFFFVEGGRLRWSPPRAHDIRDGIAFGLLVLAGMLGIYFVFLKPA
                     GILAFAAPAIQQKVMGFGLVTPARYVAFAGMISLLHSALEEYYWRWFVFGRLRDPIGP
                     LWAAIIASAAFMGHHVVILGRYFGWLSAGQILFSLAVFFGGVIWCAMYHRSRSLYGPW
                     ISHALIDAAIFVVGYDLALS"
     gene            complement(3020..>3382)
                     /locus_tag="PKI05_07845"
     CDS             complement(3020..>3382)
                     /locus_tag="PKI05_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4976 domain-containing protein"
                     /protein_id="HOA52155.1"
                     /translation="VDIYPTLCELCNLPIPEGLEGTSLVPLFEDPNRPWKKAAFSQYP
                     RSGVMGYSMRTDRYRYTEWRPRKGGEPVGVELYDHAEDPQENINLANRPEYQNLINEL
                     KKQLEAGWQGALPPKPAE"
BASE COUNT          664 a          920 c          985 g          813 t
ORIGIN      
        1 gccctcaccc caaccctctc ccggagggag agggggactt tttgggatag actctgaggc
       61 cataatttct cgcttaaaaa gtgtgaacta ggctttggtt ctgcattttc gattttcgcc
      121 atccgagaat atctataatc acctggcggg tggtcttcgt tgtccagttg atcggcttaa
      181 ggggcgagcg tggcaaagct gacaacggtg gacaccaaag gcgacaaagt tactttctct
      241 gccaagtgac ggaagaaagc cctataacca ttttggggga aatgacgccc ggacctttgg
      301 gtcgatgtgc tttttttgta ccttctaagg ctagtaattg gccgggtaga gcgtataatt
      361 tgggtcaacg catccgcaat gggattgagc caccctgccc gtgcggagcg gtgggccatt
      421 cctgaatggt ctgatttgaa actttgcgtg tggctgcgat ttggaggtta ttgctgtgga
      481 attgcccaag agacctaagg acgatcgatt caccgtccct ggaaaatatc gagagttaga
      541 acaaagtgtt cgacaaaaag ccgcttctca ggacattcct gcgctcattg tttacgcctt
      601 tgatcaccgg acgagcctcg ggccgttcgt gatggttcag aattggatga tccctggcgc
      661 gccgcgggcg ttggcttctg ccatgtatgc ggcgggattc cacaaattac gggttgtcat
      721 gcagcagtgg acgcctaacg tccgtcccag ccaggcccgc atcgacggcc aagtcccgca
      781 actgctgctg atttccagca tgcaaattca cagcgcgcgg gcgtatgagc tcatccgcga
      841 cgcttggaca ctcggagacg aacgaccttt gattatcgct gggggtgcca aggccattta
      901 cgagccgtgg gatttcttcg gtctatccga agacggctcc gtaggggcag acgtggtctg
      961 caccggggaa gagtacgtct tgatggaatt cctcgaccga cttttggact acaagctccc
     1021 gggcgagcac atccgggctg cttttgagcg ggcacgcgag caaggcgttt tcaacgacat
     1081 cccgggattg gtcttccggc ccgacccgcc gcgtgggcat ccagagtacc tcgtcgatac
     1141 aggcgtccaa cgactggtcc aagatttgga tgagttcccg ctgcccttcg atgccttgtc
     1201 gctctttgaa ccgccgcaca agcgaacaac gctgtcgccc gaaccgatcc ccgtggatca
     1261 actgggaaaa tacgccaaag tgttggccgt cgtgaccacg cacggctgca agttccgttg
     1321 tccctattgt cccattccgg catacaatca ggagtcgttc cgttatcgga gccccgaacg
     1381 gatcgtggag gagtttgcgg gcattcattc gcggacgggg attcgctatt tcttcgggac
     1441 ggatgacaac ttcttcaaca atcgccagac ggtcgaggaa gtcttttcgg ccatggcgaa
     1501 ggccaccgtc aacaacaagc cgttccgtga tgcgatcatc ttcgccaccg agggaacaga
     1561 attcgatgtc cacaagaaca ttgacctgat tccgttggga cgggaggctg ggttgcggag
     1621 catctggttt ggcatcgagg acctgacggc cgatctcgtg aagaaaggac aatcggccga
     1681 taagaccagg gtcgtgttcc gcgaactcat caagcatggc atcgccccca tgccgatgat
     1741 gatgcaccac gacaaccaac cattgtggag ttggggtagc ctgcggggtc tcatcaatca
     1801 ggtgggcttt ctccgtaaag tgggtgcaat cacctgccag atcaccttgt tgacgccttc
     1861 tgtgggaagc aagggcttcg aaaagccgtt tgaagatggg atcgttttga aaaaggtggc
     1921 tggcaaagcg gtggaagatt atcactacga cggcaaccgg gccatcgcca cggcacatcc
     1981 tagccccttg cgacggcagt tgaacgtcct tgcaggctac ctggcatttt ataacccctt
     2041 gaatctgctg cgtgccttgc cgaggctcga tcccctctgg aaggagcgga ttttcactca
     2101 gattcagggg atgatcgggg tggccaaatc gatctggaat gcgcgggagt ggttctacga
     2161 tctagccgtg ggaccgattg agtatcacac ggcaccgccg cagcccaaat acccacttgt
     2221 cacccctgtg gtgagaatac aagaaagccg tgttcctgcc atgggatgag cttggcccgt
     2281 gcgccgcgat ggcttgcctg gttatgccct ggcggtcctg cttgtggtgc tgatgacggt
     2341 cccgaccgcc gcagcgctag ggtattttgt cctgctagaa tccgccccat cgctggttca
     2401 gcaaagcgcc tacgtcgccg gcaaagttgt gatgaccttg ccggcgatct ggttcttttt
     2461 tgttgaagga gggcgattac gatggagccc tccgcgggcg catgacatcc gagacgggat
     2521 tgccttcggg ttgctcgtcc tggccggaat gttagggatt tacttcgttt ttcttaaacc
     2581 ggcgggcatc ctcgcttttg cggcaccggc cattcagcaa aaagtgatgg gctttgggct
     2641 tgtgacaccg gctcggtatg tggcattcgc cgggatgatt tccctgctgc attccgccct
     2701 ggaggaatac tactggcgct ggtttgtctt tggcaggctg cgggacccaa tcggaccgct
     2761 ttgggcagcc atcatcgctt cggcggcttt catgggccat cacgtcgtca ttctcggacg
     2821 ctacttcggc tggctgagcg ccggtcagat cctcttttcg cttgccgtct tttttggcgg
     2881 ggtcatctgg tgtgccatgt atcaccggtc acgatccctt tacggaccgt ggatcagcca
     2941 cgctttgatt gacgcggcga tcttcgtggt cggttacgac ttggccctaa gctgaggccc
     3001 ctgcccgggt ttttgctgtt cattccgccg gttttggcgg gagagcacct tgccaaccag
     3061 cttccaactg ctttttcagc tcgttgatca ggttctgata ctcgggacgg ttggcgagat
     3121 taatgttttc ctgagggtcc tcggcgtggt cgtacaattc gacgcccacc ggctcgccac
     3181 cttttcgggg acgccattcg gtgtaacggt agcgatccgt tcgcatggag tagcccatta
     3241 cgcccgagcg gggatattga ctgaaggccg cctttttcca gggccgattc gggtcctcga
     3301 aaaggggaac caaacttgtt ccctccagtc cctcggggat aggcaagttg cacaattcgc
     3361 acaatgtggg ataaatatca ac
//
LOCUS       DAOPTR010000359         3381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22391_length_3381_cov_3.469333, whole genome shotgun sequence.
ACCESSION   DAOPTR010000359 DAOPTR010000000
VERSION     DAOPTR010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22391_length_3381_cov_3.469333"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="PKI05_07850"
     CDS             <1..841
                     /locus_tag="PKI05_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52156.1"
                     /translation="ILTAWFWLAIIALLIIAYYGVYAFAFGLNDEERPIPAWRLLAGW
                     ISAGIFIVIAILFSNGLSLMAAPERWKFIWFSNQVAGAATGTGHNFGDASLWHRWPMV
                     FGMALMTTAVWSLVDAVFFDKRGTLEYRRWLAKFSLLIGIVGAVVYASAGTGYVFGTW
                     PKSVFSYMWSDWRLPLTLFTGASPGLVVATLLWIRYRGVSQGPVLLASLAQVIVLSTN
                     AVSRQLVQNIELTPLVSLNQLATKPDWGPMALFLVSFAIGVGIIAWMLQQVVRAERSQ
                     VEQ"
     gene            1555..>3381
                     /locus_tag="PKI05_07855"
     CDS             1555..>3381
                     /locus_tag="PKI05_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52157.1"
                     /translation="MNARVRIYQGMVAFCLAFLILNCPALGQGVGQGTDNGPVANADQ
                     LRRQCDDLLARARQAIAEGNLNGAEQLLHQAEQLGVNYPPLYLGDTPAKVRKALESAR
                     QAQPAGQTMPQSPFDARGLGQTPDPKTQAKNYVRNGRMALARGNLSEAAQWCQMAAGL
                     PATFAPEEDSPSKLAEDIRRAGGQVAGATPGAVTSLPPVGQVTQVAPPANASQKSPAQ
                     QQSDQWLLEARRALALGDVRTARRYVEQVRALGLTYGRYDDSPDRVEALIQTHENLLS
                     QKAERGQTEGWRRLYAKLLLEEADGLLRWGDLDEAERLAQLAAQQGVTFSPFEMKPET
                     ILERAANERRNRGLNPALAAQKQRAMQLLAQARSSLAMSDVSTAETLAHQILALNLPE
                     SVFSPNEDSPARLISDIQQAKQAGAVRPASAQAVVPASGPYSGNVAAAAVYDPTRDWT
                     RNVPASTSQPAPTSRGMMLVQQGEDALRRGDYAAAIDYFRQATAYLHELDPATAQRVQ
                     DVLQQDAARTAATNVMSGNVPAAPDPLAQQQIAARQLATEVAQRESRALALRDSNPQQ
                     GMQMLQDTRKMVETAAVDPSTRDQLLRRLDRQIADLQQYIDQN"
BASE COUNT          725 a          964 c          968 g          724 t
ORIGIN      
        1 cattctcacg gcttggttct ggttggcgat catcgctctg ttaatcattg cctattacgg
       61 cgtttacgcg tttgcgttcg gactgaacga cgaggaaaga ccaatacccg cctggcgttt
      121 gctagccggg tggatttcag caggaatatt cattgtcatt gccattttgt tttccaatgg
      181 gctgagtctc atggccgcac cggagcggtg gaagtttatt tggttcagca accaggtcgc
      241 cggcgctgct acgggaaccg gccacaactt cggtgatgcc tcattgtggc accgctggcc
      301 catggtgttc ggaatggcac ttatgacgac ggcagtttgg tcgctggtcg atgcggtatt
      361 tttcgacaag cgagggacgt tggaatatcg tcgctggctt gctaagttca gtctcctaat
      421 tggtattgtt ggggctgtgg tgtacgcaag tgcagggacg ggctacgtct tcgggacgtg
      481 gccgaaatcc gttttttctt acatgtggag tgactggcga ttaccgctca cgctgttcac
      541 gggtgcctca ccgggcctgg ttgtggccac acttttgtgg attcggtacc gtggggtttc
      601 ccaaggacct gtgctgttag ccagtctggc ccaagttatt gtcctctcca ccaacgccgt
      661 cagtcgccag cttgtccaaa acatagaatt gacgccacta gtgtctttaa accagttggc
      721 aaccaagccg gattggggac caatggccct gtttctcgta agtttcgcga tcggggttgg
      781 gatcatcgct tggatgttgc aacaagttgt gcgggctgaa cgatcacaag tcgaacagta
      841 atccaacgac ggttcaagca ataaccacaa gcacgttgtt tgggtggcta accgcggttc
      901 tggtctctct gactatttcc ccacggccga ctcgagtgtt ttctcggctc cccgttcgac
      961 acgtttagca cctttcctgc gaggtatttc tctttcttcg tcttgcctgt cgtctcctct
     1021 gtcttgtcat tcggaaggca tcggccgggt aaaacccgac acttcctgga atcttttcat
     1081 ttttggtgtt gacggcaggg ctagaagacc gttattcttc gccaccattt ttgtgaaaag
     1141 tcgcattggt tggtcacgga taattaggga aacgtggaag actcaggccg cattcaccgc
     1201 gaatgaccaa caccggggag ctgtgagaag aggttagttc agggggaaca agacaacgcc
     1261 gcacgtgaaa acgaagttcg agagaaacca gacgattcga tcttgaccca acatgtgacc
     1321 gtcggtatta atcccgcacg tagacttgcc gacgcggtgg agaataccca atcctcacca
     1381 aaggtcattg ctccagaggg atggagctgg tgagtgattg cggctggtag ggaatcgcat
     1441 ggaggcgata ccgctatgcc gtttttcaat cgacgcatat cagggtctca ggattccatg
     1501 aagacaggaa caagtaacgg acggcttgtt ggtgatctcg aaaggagaac tgggttgaac
     1561 gcgcgtgtac gaatctacca gggaatggtg gccttctgtc tggcgttcct cattttgaat
     1621 tgtccagcct taggccaagg agtcggccaa ggtacagaca acggccctgt ggcaaacgct
     1681 gaccaactcc ggcggcaatg cgatgatctt ttggctcggg cccggcaggc cattgccgaa
     1741 ggaaacctca atggcgccga gcaactcctt caccaggcag aacagttggg tgtgaattat
     1801 ccgccgcttt atctgggtga cacacctgcc aaggtccgca aggccttgga aagtgcccgc
     1861 caagcacagc ctgccgggca aaccatgccg caaagcccct ttgatgcccg cggactcggt
     1921 caaacgcccg atccaaagac gcaggccaag aactatgtgc gaaatgggcg aatggcacta
     1981 gcccgcggaa atctgagtga ggcggctcaa tggtgccaaa tggcggccgg attgcctgcg
     2041 accttcgccc cggaagaaga ttcgcccagc aaattggcag aggacatccg gcgtgcaggt
     2101 ggacaagttg ccggggcaac acctggtgcc gtaacctctc ttcctcctgt aggtcaggtg
     2161 acacaggtcg cccccccggc gaacgccagc caaaagtcgc ctgcccagca gcaaagcgac
     2221 caatggcttc tcgaggcacg tcgggcctta gccctcggtg atgtgcggac ggctcgccgc
     2281 tatgtggagc aagttcgtgc tctgggattg acctacggtc gatacgatga ttctcccgat
     2341 cgggttgaag cgctcattca gacgcacgag aatctccttt cgcaaaaggc ggaacgaggc
     2401 caaacagagg gttggcgacg cctctatgcc aagttgctcc ttgaggaggc cgacggcctg
     2461 ttgcgttggg gtgacttaga cgaggccgaa cgcctggccc aacttgcggc ccaacagggt
     2521 gtgaccttca gcccgttcga aatgaagccg gagacgattt tggaacgagc cgccaatgaa
     2581 cgtcgaaacc gggggctgaa tccggcccta gcggctcaga agcagcgggc catgcaactc
     2641 ctggcacaag cgcggtcgtc cctggccatg tctgacgtga gcactgccga aactcttgcc
     2701 caccaaatcc tggccctcaa tctcccggaa agtgtgtttt ctcctaacga agattcacca
     2761 gcccgcttga tcagcgacat ccaacaggcc aagcaagccg gtgccgttcg tccagccagt
     2821 gctcaagcgg tggtccccgc ctccggacca tacagcggta acgtcgcagc agctgcggtg
     2881 tatgatccca cgcgggactg gacccgaaat gttccggcct ccacatctca gcctgcccca
     2941 accagccgcg gaatgatgct ggtccaacaa ggggaagatg ccctccgtcg cggagactat
     3001 gccgctgcca tcgactactt ccgccaagct acggcttacc tccacgagtt ggaccccgcg
     3061 accgcgcaac gtgtgcagga cgtcctccag caagacgcgg cccggacggc ggccacgaac
     3121 gtcatgtcgg gcaacgttcc ggcggccccg gatccgcttg cccaacagca aatcgcagcc
     3181 cgacaactgg ctacggaagt cgcgcaaagg gaatcgcggg ctttagccct gcgtgactcc
     3241 aaccctcaac aggggatgca gatgttgcaa gacacacgta agatggtcga gaccgcggcg
     3301 gttgatccgt cgacccgcga ccagctcctc cgccgactcg accgtcaaat cgccgatctg
     3361 caacagtaca tcgatcagaa t
//
LOCUS       DAOPTR010000360         3376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22427_length_3376_cov_3.349895, whole genome shotgun sequence.
ACCESSION   DAOPTR010000360 DAOPTR010000000
VERSION     DAOPTR010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22427_length_3376_cov_3.349895"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="PKI05_07860"
     CDS             <1..924
                     /locus_tag="PKI05_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52158.1"
                     /translation="WFMKPEIKLVNPFGGLDKVRMNPPASSPDLLEPAGPVDPQVCFI
                     SIQSRDGRPIALLANYSLHYVGGVGPGHISADYFGVFADRIQELLKADRLEPPFVGIL
                     SNGASGDVNNISFQKPRPAKKPYEQMRYVAYDLAQKVFEAHQDLQWQDWVPLGMLQQE
                     LVLKVRKPTPEQLKRAKEILAGAPPLPERPHEETYARRLVQLAESPDTVSVPLQAMRI
                     GEVGITAIPFEVFAEIGLEIKEKSPFRPTFTISHANGSYGYLPTPRQHELGGYETWLG
                     TCKVEEQASVKITETVLGMLRALRGESTNNP"
     gene            complement(956..2935)
                     /locus_tag="PKI05_07865"
     CDS             complement(956..2935)
                     /locus_tag="PKI05_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOA52159.1"
                     /translation="MLRLLWTSQGVSHRLGSRAWVWVVVAATVCFAFSSLCQAEESHL
                     LAYWSFDESDQRVAHDMSGNDFHLLLQSARRGSGISGQALVLKGSDSVMLAEAFDEFF
                     VLSDDYAVEAWVKHDQKTPQIYLSKWTGTGMGSAWWMGFFEGCVQWAEYNDQFQVRLK
                     GPDIADGRWHQVVGVRSGKQLSLFVDGELITQRVTPGSPAGHNAAPLKIGGFGAGRRS
                     SWPLVGSIDEVRIYCRALSAQEINERFKLISSGQQGPVLQPIANGLPARTIIRLDVPS
                     LIEAGKSRSFSLVLVSNRPFSQDTLTARFSIKDNGGRLLKEWQLPISPKAGSRVSNHP
                     VELPALPEGTYDLEAAIGSQGASGKIQVKNLEPTVAYNLRIKNERAKKSAFYRGIVSA
                     YSGMRYKPDGTPDVEAMLGHLSHLGVNVYTYLIAPRSSQELAALGEFCQKARSAGIEV
                     WVYLVPPSEAPIDRDKPIRERRYPPFDMDYVRWAEAVAQVSLDHPNLTLWMIDDFDGN
                     LGFFTREYTQQIYKTSKQINPRLLFGVCVYHESLANFVKAGYLDYVDALLWGYQHSSA
                     RYPECGLDATTLPLEIHDYLQTGKIAIPCIYFTPHSSWTEGRPTKAYLEEAMKTAFEQ
                     AGIVWVFTTPRPGTMQEEVVRAFTQRHPLPKWPGQ"
     gene            complement(3015..>3376)
                     /locus_tag="PKI05_07870"
     CDS             complement(3015..>3376)
                     /locus_tag="PKI05_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52160.1"
                     /translation="LFINEVPANGKTTCLISPIRTLPVVSIKIGQPSLELNGQRLIFP
                     VGLTSGGYLEFDGQNPARVYDERGKLTQTVDVEGTVPVLNAGNNTIHFTCQPEVPPGV
                     HPRVKVTVITMGDPLSP"
BASE COUNT          770 a          938 c          933 g          735 t
ORIGIN      
        1 tggttcatga aaccggaaat taaattggtc aatccctttg gtgggctaga taaggtgcgg
       61 atgaatccgc cggcaagtag tcccgatctc ctcgagcccg ccgggcctgt ggacccccaa
      121 gtctgcttta tctcgattca gtcgcgtgac gggcggccaa tcgcactctt ggccaattac
      181 tcgctgcatt atgtgggcgg cgtcggaccg gggcacattt ccgccgatta ctttggtgtc
      241 tttgccgacc gaattcagga gttgttgaaa gccgatcgac tcgaaccgcc ctttgtgggc
      301 atcctttcaa atggagcaag cggtgatgtg aacaacattt ccttccagaa accccggcct
      361 gcaaaaaaac catacgagca gatgcgctat gtggcctacg acttagctca gaaggtgttc
      421 gaggcacacc aggatcttca gtggcaggat tgggttccct tggggatgct gcagcaagag
      481 cttgtcctca aagttcgcaa accaacgccg gagcaactga agcgggcaaa agagatcctg
      541 gccggggcgc cgcctttgcc ggagcggcct cacgaagaga cctatgcccg ccggctggtc
      601 caattggcag aatcacccga tacggtctcc gttccgctgc aagccatgcg aattggggaa
      661 gttggcatca cggccatacc ttttgaggta tttgccgaaa tcggcctgga gattaaagag
      721 aaaagcccct tccgccccac gtttacaatc tcccatgcca acggctcgta cggatatctt
      781 cctaccccac ggcaacacga actgggcggt tacgagacct ggttgggaac atgcaaagtg
      841 gaagagcaag catccgtcaa aatcaccgaa acggtcctcg gcatgttgcg ggcacttcgc
      901 ggagaatcca cgaacaatcc ttgatcacgg cttgtctgtg gtgaccaggc tgagtttact
      961 ggccgggcca tttcggtaac ggatgccttt gcgtaaaggc gcggaccact tcctcttgca
     1021 tggtgccggg gcgaggggtc gtgaagaccc aaacgatccc cgcctgttca aaggccgtct
     1081 tcatggcctc ttccagatag gccttcgttg ggcggccctc agtccacgag gaatgcggcg
     1141 tgaaatagat gcacggaatc gcaattttgc ccgtttgcag gtaatcgtgg atttcaaggg
     1201 gtaacgtggt tgcgtctagc ccacactcag ggtaacgggc ggaactatgt tgataacccc
     1261 ataacagggc atccacataa tccaaatagc cagctttgac aaagtttgcc aggctttcgt
     1321 gatacacaca aacgccgaac aggaggcggg ggttgatttg tttcgacgtt ttgtaaattt
     1381 gctgggtgta ttcacgcgtg aaaaacccta aattgccgtc gaaatcgtcg atcatccaaa
     1441 gggtcaggtt ggggtgatca agcgagactt gggcgaccgc ctcggcccag cgcacgtaat
     1501 ccatgtcaaa cgggggataa cgcctctcgc ggataggttt gtcccgatcg ataggggcct
     1561 ctgacggtgg cacgagatac acccagactt cgatcccagc cgatcgtgcc ttctggcaaa
     1621 attcccctag agcggccaac tcttgtgagg accgcggtgc gatcaaatac gtgtaaacgt
     1681 taactcctaa gtgactcaaa tgaccgagca tggcctccac gtcgggcgta ccatccggtt
     1741 tgtagcgcat accggagtaa gccgagacta tcccgcgata aaaggcgctt ttttttgccc
     1801 gctcgttttt gatgcgaaga ttgtaggcga cggtgggctc caggttcttc acctgaatct
     1861 tgccggaagc tccctgagaa cctatcgcgg cttcgagatc gtaagtccct tcgggcagcg
     1921 ccggcagttc taccggatga ttactgacac gggaaccggc cttgggcgag atgggtaatt
     1981 gccattcctt gagcagtctg ccgccattgt ccttgataga gaatcgggca gtaagtgtat
     2041 cttgcgaaaa aggccgattg ctgaccagaa ccagcgagaa gctgcgactc tttcctgctt
     2101 caatgagaga gggaacgtca agccgaatga tcgttcgggc cggtaaacca ttagcgatgg
     2161 gttggagtac gggaccctgt tgaccgctgg agatgagctt gaacctctcg ttgatttctt
     2221 gggcggagag tgcccgacaa tagatgcgga cctcatcgat ggacccgacc aggggccaag
     2281 agctccgccg accggcgccg aagccgccga tcttcaaggg ggccgcatta tgccctgctg
     2341 gactcccggg agtaacccgc tgggtgatca gctcaccatc gacaaagagc gacaactgct
     2401 tgccagaccg cacaccgacc acctggtgcc agcgaccgtc ggcgatgtct gggcctttca
     2461 atctcacctg gaattgatcg ttgtactccg cccattgcac acatccttca aagaaaccca
     2521 tccaccaagc ggaacccatt cctgtacccg tccacttgga caggtagatt tggggtgtct
     2581 tctgatcgtg tttcacccat gcttccactg cgtaatcgtc agagagaaca aagaattcgt
     2641 cgaaggcttc ggcaagcatc acggaatcgc tccctttcaa gacgagagcc tgaccgctga
     2701 tgccactccc gcgccgtgcg gattggagca aaaggtggaa gtcgttgccc gacatatcgt
     2761 gggcaacccg ctggtcggat tcatcgaagc tccaataggc caggaggtgg ctttcctcgg
     2821 cctggcacag tgaagaaaag gcaaagcaga ccgtggccgc cacaacgacc caaacccatg
     2881 cccgtgatcc aagccggtga ctcacccctt gcgatgtcca caggaggcga agcataggaa
     2941 acctctcact ttgctctttc ggaacgtcaa atctctgcta ccaacgacga accggaatat
     3001 gcttggccaa tcatttacgg tgacaaagga tcgcccatcg tgatcacggt gacctttacc
     3061 cgtgggtgca cgcccggcgg gacttccggc tggcaggtga aatgaatcgt gttattccct
     3121 gcgttgagca cgggaacggt gccttccaca tcgactgttt gcgtgagctt tccgcgttca
     3181 tcataaacgc gggctggatt ctgaccatcg aattccagat atccaccgct ggtcaggcca
     3241 acaggaaata tcaggcgttg cccatttagt tccagcgacg gttgcccaat cttgatggaa
     3301 accacgggca aggttcggat aggactgatc agacacgtcg tttttccgtt tgcgggcact
     3361 tcattgatga agagat
//
LOCUS       DAOPTR010000361         3371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22485_length_3371_cov_3.012364, whole genome shotgun sequence.
ACCESSION   DAOPTR010000361 DAOPTR010000000
VERSION     DAOPTR010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22485_length_3371_cov_3.012364"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1066)
                     /locus_tag="PKI05_07875"
     CDS             complement(170..1066)
                     /locus_tag="PKI05_07875"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HOA52161.1"
                     /translation="MTPEERQAAVERILRSPSYRVAYQDLDFLTDPKLRATRMELELL
                     KPELAFEREKINSTVVVFGSTRIVDEPEARQLVEKAEADLKADPENPKLRRAYERARS
                     LLEKSRYYELARRFAYLVSQSCANNGQCDYVICTGGGPGIMEAANRGAYEAGAKSIGL
                     NIRLPMEQIPNPYITPELCFQFRYFALRKFHFLLRAKALVVFPGGFGTLDELTDALTL
                     RQTGRMQPIPIIIFGREYWDRVLNFQALADEGVIDDEDLKLFEFVETAEEAWEKICRF
                     HHLSMLEAKPVYWENSGGIDPH"
     gene            complement(1288..>3371)
                     /locus_tag="PKI05_07880"
     CDS             complement(1288..>3371)
                     /locus_tag="PKI05_07880"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52162.1"
                     /translation="RTKLYREIRWFGRSLLLENRLFADRPIVFLKTRRFLCQMLHEYI
                     AYFYSAADLHGGGVYVLKKPGESLEIEDLTTGHFGRGIFQSLALSYDAKTVYFAFAPI
                     PAGDRQRPLRPDWSVLTAGPLPPEFAFESPDRPCFHLFAIDLTNRTIRQLTDGRYDDT
                     MPWPLPDGDLVFMSTRRGGYCRCNNPWEPIQTYTLHRLNPKTGAIRTISYHETNEWHP
                     AVLNDGRIAYCRWDYVDRSAAHFHGIWVTSPDGTNARSVFGNYTMEISTCFQPRPVPG
                     SRKIAFIAGAHHAVVGGSLVLFDPDKARYDPETGEDVFDSLEVLTPEIPFPETPSEWP
                     TGYVVSPWPLSEDFYIISFGHDRLPGMGSGNRSESGVGIYYFDRWGNLELLYEDPGWA
                     CLDPVPVIPRRPPPVVQETFDPDLGQEAEVVVYDVRQSHYPLPPDRPIRELRVFQLLP
                     KATHPVNNPRIGHANAENARALLGTVPVEEDGSAYFRVPADRPLYFQIVDDRGRAVQG
                     MRSVTYFRPGERRGCIGCHEPPNSSVPTRDILALRREPSALTPGPDGSLPFSFPRLVQ
                     PVLDQFCIRCHSPNAGPQFAAPDLMGLPQGEFTRSYVNLRPYLRWYEWGGGSISQIVT
                     RPGQLGADASPLLNILENPTHREAVTLDRESLLRLTLWLDANVPFYGTYDNDQRAQQR
                     AGQLIAMPELQ"
BASE COUNT          765 a          990 c          944 g          672 t
ORIGIN      
        1 cggcagttga ttatgtccca cggcgacttc cggcctaagg agcccccgtg gcgccgtttg
       61 tccggcgaga ggccgcgtgc cctcccttcc ttatactcat ttggaaaggc gaaaaggcca
      121 acatactggc cgcggtctgg ggggtcactg gacctactat ttgggatcat cagtgggggt
      181 caataccccc cgaattctcc caatagaccg gcttagcctc gagcatcgac aagtggtgga
      241 agcgacaaat cttttcccaa gcctcctcgg ctgtctcaac aaattcaaaa agttttagat
      301 cctcatcatc aatgacaccc tcatcggcaa gggcctggaa attcagcact cggtcccagt
      361 attcccgccc aaaaatgatg atcgggatcg gctgcatccg accggtttgc cggagcgtca
      421 aggcatcggt cagttcgtcg agggttccaa agccgccggg gaagacgacc agggccttgg
      481 cccgcaggag aaaatgaaat tttcgtaagg caaaatagcg gaattggaag cacaactctg
      541 gcgtaatgta cggattgggg atttgctcca tgggcagccg gatattcagc ccgatcgatt
      601 ttgctccggc ctcataggcc cctcggttgg cggcctccat aattccgggc cccccgcccg
      661 tgcaaatcac atagtcgcat tgcccgttgt ttgcgcagga ttgcgaaacg agatatgcga
      721 accgtctggc cagctcgtag taacggcttt tttccaggag gcttcttgcc cgctcgtaag
      781 ctcgccgcaa tttcggattt tccggatctg ctttaaggtc cgcctccgcc ttttcgacaa
      841 gctgtcgggc ctccggctca tcgacgattc gcgtacttcc gaacactacg acggtggaat
      901 tgatcttctc ccgctcgaac gccaattccg gcttaagcaa ctccaattcc atccgtgtgg
      961 cgcgcaactt gggatcggtc aggaaatcca agtcctgata cgccacccga tagctgggcg
     1021 acctcaaaat tcgctcgacg gctgcctgac gctcttccgg agtcatagtt tcccactcct
     1081 catcgtcaac ttgtcaattg ccccctccga tcaccacaat ctcgattcac ctactgcaaa
     1141 ttacccagaa atggcgattt ctccaaggcg aatccaccaa gctgcgtggg gtatcaaggc
     1201 taagcagaaa aagaaagaaa agccgcgggt gccatgcccc ctagtagtgg gtcatggcgt
     1261 cgaccagggc gacggacccc aacacaacta ctgcaattcc ggcatggcaa tcaactgccc
     1321 ggcgcgctgc tgcgcccttt ggtcgttgtc atatgtccca taaaacggga cgttggcatc
     1381 gagccaaagg gtcaatcgca aaagcgactc cctatcaagg gttacggcct cgcgatgagt
     1441 cgggttctcg aggatattca gcaacgggct ggcgtctgcc cccagttggc cgggccgcgt
     1501 gacaatctgc gagatgctgc ctccgcccca ttcgtaccag cgtaaatacg gccgaaggtt
     1561 cacatacgac cgcgtaaatt ccccctgcgg aagccccatc aagtcggggg cggcaaactg
     1621 gggaccagca ttcggcgagt ggcatcggat gcagaattga tcaagcacag gctgaaccag
     1681 ccgggggaag gaaaacggca gcgaaccatc cgggccgggc gtcaaggcag agggttcacg
     1741 tctcagtgcc aagatgtccc gggtcggaac cgaactattg ggcggctcgt gacatccgat
     1801 gcacccccgc cgttcgcctg gtcggaagta tgtcaccgag cgcatcccct gaaccgcccg
     1861 gcctcgatca tcgacaattt gaaaataaag gggacgatcg gccggaacgc gaaagtatgc
     1921 cgaaccgtcc tcttccaccg ggacagtccc caagagggca cgggcattct ctgcgttggc
     1981 gtggccgatc cgcggattat tgacgggatg ggtcgccttg ggcaaaagct gaaagacgcg
     2041 aagctcgcga attgggcgat ccggcggcag cgggtaatga ctctgacgca catcatacac
     2101 caccacttcg gcctcctgcc ctaaatcggg atcaaacgtt tcctgcacca ccggcggcgg
     2161 cctgcgggga atcacaggta cgggatccaa gcaggcccat cctgggtcct cgtaaaggag
     2221 ttccagattc ccccagcgat cgaaatagta gattcccacc ccagactctg aacgatttcc
     2281 cgatcccatg ccgggcagcc ggtcatgacc gaacgaaatg atatagaagt cctctgacag
     2341 cggccaagga ctgaccacgt aaccggtggg ccactcgctg ggcgtctcgg gaaacggtat
     2401 ctctggtgtc aagacttcca gcgagtcaaa cacgtcttcg ccggtttcgg gatcataccg
     2461 ggccttgtcg ggatcaaaaa gcacaagtga tccgccgacc acagcgtggt gcgccccggc
     2521 aatgaaggcg atcttccgag accccggaac tggccgcggc tggaagcacg tgctgatctc
     2581 catggtgtaa ttgccgaaaa cgctgcgggc attggtgcca tccggtgatg tgacccaaat
     2641 gccgtggaaa tgggccgccg aacgatcgac atagtcccag cgacagtaag cgattcgccc
     2701 gtcgttgagg accgccggat gccactcgtt ggtttcgtga taggaaattg tgcggatggc
     2761 accggttttg gggttgagac gatgtaacgt gtacgtttga ataggttccc aaggattgtt
     2821 acaccgacag tagccaccgc gccgtgtgga cataaagacg aggtccccat ccggcagtgg
     2881 ccagggcatc gtgtcgtcat agcgaccatc ggtaagctga cggatcgtgc gatttgtcaa
     2941 atcgatggcg aacaagtgaa agcacggccg gtccggcgat tcaaaggcaa attctggcgg
     3001 cagcggtccg gcggtaagca cgctccaatc cggccgaagc ggtcgctgtc gatcgcccgc
     3061 ggggatcggc gcaaaggcaa agtacactgt tttcgcatca taggaaagtg cgagcgattg
     3121 gaagataccc cggccaaagt gacccgttgt tagatcctca atctccaagc tctctcccgg
     3181 tttcttcaga acgtacaccc ctccgccatg aaggtccgcg gcactgtaaa agtaggcgat
     3241 gtactcgtga agcatttggc agaggaaccg ccgtgtcttc aaaaagacaa tcggtcggtc
     3301 ggcaaaaagc cggttctcca agagaagact gcgtccgaac cagcgaatct ctcgataaag
     3361 tttggtccgt t
//
LOCUS       DAOPTR010000362         3367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22521_length_3367_cov_2.611715, whole genome shotgun sequence.
ACCESSION   DAOPTR010000362 DAOPTR010000000
VERSION     DAOPTR010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22521_length_3367_cov_2.611715"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(729..802)
                     /locus_tag="PKI05_07885"
     tRNA            complement(729..802)
                     /locus_tag="PKI05_07885"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(766..768),aa:Met,seq:cat)
     gene            1369..3117
                     /locus_tag="PKI05_07890"
     CDS             1369..3117
                     /locus_tag="PKI05_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOA52163.1"
                     /translation="MHSVGRFLVLLSSFITLWHASLTMGQAVPSIWYEDMGTARRVAA
                     ETNRLLLIHFWAEWCRPCRQMEAEVFSRPDVLSALATNYVAVKVNFDRAPELVREFRI
                     ESIPTDVILTPNGELLQKFVGGSSAERYIERISQVAYTYQQRRTMLARTNTPSATPPL
                     TPSYATSPWSSPSPQGTAAATRPVDQPPTMAMNAGVGLPQSGPGGMPPSSQGSYFSGQ
                     PLASAPAPVGPGQIPAGPSGFGQPQNQVLPQPPAGLSPWSNSSQLATTSTPLAPPPDT
                     SFQASPSPPQSIQGYATSSPPSSANVGPSQSFGPPPQVAQGPSSSPPMMEAQPPANSM
                     ASTILARQSPKPKVEIPPGNPPLALEGYCPVSLTDKQRWVLGNPRWGARHEGRTYLFA
                     GPEEQQKFLANPDRYAPVLSGMDVVQLVEATQLTEGRREHGAWFGGRVYLFSSEESYQ
                     KFSLDPYRYINALPQAVARLSQQIQGEASLTTSPGNVAGTNVPPQQPPVGMGQLPVGN
                     PPLRPTVSSPMAPAISPPTSPPVDTVGGVPTASATSWAPPPTVNIQLPPSVQAVANGT
                     ASPDQPRWPVAQRPRW"
BASE COUNT          705 a         1016 c          921 g          725 t
ORIGIN      
        1 tttgcgattg gccgcgcggc cggtgtatcc gattcgcttc gcgccgtttg gtcccgtccc
       61 cgtccgctta ggagaccgcc gccattatat cccggcgggc cgatgcccca ccagcctagg
      121 aaactggccc aatccttgtt cggacacggt cttagccggc gtgtcgtaaa ccgtcagaaa
      181 gcccccgcag caggatgaga accgtccgag gattgtgttc ctaataaggc atgtgaggcc
      241 ttagcgccaa tgagaacgag gttgagtgcc catttcaggc gggcacactt ttttaatggt
      301 ccccggcaaa cggcgggatg gcgtaaccgc accctagggg ggtacattat ggcccaaatt
      361 gctaaatcag cctaaaccgg cgattccagc gacgagggtc tctgttcgca gcgatgataa
      421 ttgccaaatg atcaggtggg actctccaag ccccaagccg tcaggccttg ccatttttgt
      481 cactaccgcc tgacacaact gacagagatc ctgtgacggg cttaaagcca tcccataatc
      541 tcccttcacc cgttcggcgg gacagggatc ggggatgaga gtggctggaa tccctgccgg
      601 ttttctcgat cgactccgac ggttccttac cctcacccca accctctccc ggagggagag
      661 ggggactttt taggatagac cctgaagtat caattacgcg cacacgatat tgtgtggccc
      721 gctgtcattg ccggcgcagg gactcgaacc ccggacacgc ggattatgat tccgctgctc
      781 taaccaactg agctacgccg gcaggtcttg gccgaccgag ggcctttact gaaacataac
      841 ttctcgacca gatgcggaca agggggcgga atggcgtaac ctcccccctt ttgacttacc
      901 aactctgttc gagtcgaact caccgagctt aaatcgccgt gaccccgagt tcgggaaacc
      961 ttttcgcatt caggcgcaca aggtagcggg acttatgaac atccacttca taccagctaa
     1021 cgccctcacc ggcggttggt cctcacctgc tgtcccattt cgtctggcaa aacggggcaa
     1081 tcaggaaaag atagcagtga aaaggatact tcaccggtcc acaaggaaaa gcgtcagccg
     1141 cgcctgttgc aactgcaata aaacggtttt ccggcaaatt cggaacattt tcacaaaatt
     1201 gactgacccc atcagtccga taaaaaaagg gcggtcatta ggtgtgtgat gccctttttg
     1261 gctgttggag tttagggagc ttgtgtgggc aatttaagtt gacgatgctc gccacagttt
     1321 tgggcgtcgt tggtcgtttc gtcactccaa caaacgagga ttgttccgat gcatagcgtc
     1381 ggccggtttc tggtgctatt aagttcgttt ataacactct ggcatgcgtc ccttaccatg
     1441 ggccaggcgg tcccctcaat ttggtatgag gatatgggca cggcccgacg tgttgccgct
     1501 gagacgaatc ggctcctttt aattcatttt tgggcggaat ggtgtcgtcc ctgtcgtcaa
     1561 atggaggcgg aggtcttttc tcgcccggat gtcctatctg ccctggccac aaattatgtg
     1621 gcggtgaagg tcaatttcga tcgggctcca gaacttgtgc gggagttccg aatcgaatcg
     1681 atcccgaccg atgtcattct tacgcccaat ggagaactcc tgcaaaagtt tgtgggtggg
     1741 tcctcggccg agcgctacat cgaacggatc agccaggttg cgtacacata tcaacaacgg
     1801 cgtacaatgc tcgcaaggac aaataccccg tcggcaacac cgccgcttac gcccagctac
     1861 gctacctccc cttggtcgtc accgtcaccc cagggtactg cagcagccac gcggccggtg
     1921 gatcaacccc cgacaatggc catgaacgcc ggtgtcgggt tgccgcagtc tggaccgggt
     1981 ggtatgcctc ctagtagcca aggtagttac ttcagtgggc aaccattggc ctctgccccc
     2041 gctccggtgg ggcccggtca gattcctgct ggtccttctg gctttggcca gcctcaaaac
     2101 caagttctcc cccagccgcc agcaggtttg tccccatgga gtaacagtag ccagcttgcc
     2161 acgacaagca caccccttgc cccacctccg gacacatcgt tccaggcgtc gcccagtcct
     2221 cctcaaagca tacaaggcta cgcgacttct tcgccaccat cgtcggccaa tgtgggaccg
     2281 tcacaaagtt ttggccctcc accccaggtg gcccaagggc catcgtcatc gccgccaatg
     2341 atggaagcac agccgcccgc caattccatg gcaagcacca tcctggcacg tcagtcacca
     2401 aaacctaagg tcgaaatccc acccggcaat ccacccctgg cgctcgaggg atattgcccg
     2461 gtgagtttga ccgacaagca acgctgggtc ttaggaaatc ctcgatgggg ggcacggcac
     2521 gaaggtcgca cctatctctt tgccgggccc gaggagcaac aaaagttcct tgccaacccg
     2581 gatcgttatg ccccagtgct ttcgggtatg gacgtggtcc aactcgttga ggccacccaa
     2641 ttgacggaag gccggcgcga acatggggca tggtttggtg gacgcgttta cctctttagc
     2701 agcgaagagt cctatcagaa gtttagcctc gatccctatc ggtacatcaa cgcactgccc
     2761 caggctgttg cccgtctatc acagcagatt cagggagagg cgtctttgac aacctcgccg
     2821 gggaacgtag cgggtaccaa tgttcctccg cagcagcccc ctgtggggat gggtcaactt
     2881 cctgtgggaa atcccccgtt aagaccgact gtctcatctc cgatggcacc cgcaatcagc
     2941 cccccgacaa gtccacctgt tgatacagtg ggtggggtac cgacggcaag tgcgacttcg
     3001 tgggccccgc cgccgacggt caacattcag cttccgccgt ccgttcaggc cgtggccaat
     3061 ggaacggctt cacccgacca gccccgctgg ccggtggcac aacgcccgcg ttggtgatgg
     3121 aaaagcactc tcggaaaaag cgaccatctt tgcagcgaag cgtgccgttt acgtgagggc
     3181 cgtcggtagc gacgaccact tggccgctct accgaaggag gctaagccgg ttcttgagag
     3241 gggctcactg gctgcgggcg gcgaggctgg aagagccttc cgcttgtgcg gcttttctcc
     3301 cttcgatccg gcatttttgc aaggatagta aagcgaacgc cgtgccgtac tgttgatgat
     3361 agtcgta
//
LOCUS       DAOPTR010000363         3362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22566_length_3362_cov_3.702449, whole genome shotgun sequence.
ACCESSION   DAOPTR010000363 DAOPTR010000000
VERSION     DAOPTR010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22566_length_3362_cov_3.702449"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PKI05_07895"
     CDS             complement(<1..393)
                     /locus_tag="PKI05_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52164.1"
                     /translation="MFTSDRIRLLLACLVAGGGLLGSIRATAEEEEVVGKRPYEMDWA
                     NRFEDTRPPLIDFEDLEGWTLEARDAVADFRRSREQQIWGKYVGKLTYRGVGPKPVVI
                     IRPPQAVAFRGPVDCVNVWIYGNNWAWVP"
     gene            392..604
                     /locus_tag="PKI05_07900"
     CDS             392..604
                     /locus_tag="PKI05_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52165.1"
                     /translation="MALLPNAKANFIFAGMLLLVGRLAKAPPAKHNGFRPAPIMAMPL
                     GVGERLYTVKYEETLSCDIIGDEISA"
     gene            571..2274
                     /locus_tag="PKI05_07905"
     CDS             571..2274
                     /locus_tag="PKI05_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52166.1"
                     /translation="MRHHWRRNLSVMIIVIMSNLAVFFLGARTAIAQQASYPERWVWV
                     FGWGLGRDSDVTEVLNLLDRAGRSGYTGAAVSFGLDSLCKWDDARFRRLEVVKEACAR
                     NHLELIPSIFSVGYGGGILGHNRYLAEGLPVKDALFEVHGQEATLIPDPPVQIQNGGF
                     EEYSGQRFTGWRFHDQPGEVSFVDTQVKHSGQASLRMENFRANQHGHGRVMQEVKVHP
                     YRCYKMTLWVKTEDLQPTSAFMVQVLADNRALAPIGFNLSPTMDWTKLVLVFNSHRFE
                     VVRVYAGVWGGRSGKFWIDDWSIEEMGPINVIRRPGTPVSVKSEDGSVVYEEGRDYQR
                     LEDPNYSPYRVDRPAPPLRLLPGSRIKDGQRLRVSWYHAQKIHESQVTVCMAEPELYE
                     IFDHEAALLAKHVNPKKVILSMDEIRMGGTCEACRGRDMGQLLGECITKQVQILRKYM
                     PDLEVYIWSDMLDPNHNAHGDYYLVEGDFTGSWNHVPKDLVIAVWGGEPRPKSVQFFA
                     EQGFSQLIACYYDAPNLEGVKGWLNVVQGVPKVKGFMYTPWRKSYDLLEEFGRLVSGK
                     Q"
     gene            complement(2612..3196)
                     /locus_tag="PKI05_07910"
     CDS             complement(2612..3196)
                     /locus_tag="PKI05_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOA52167.1"
                     /translation="MVRTASTMMLPLGAPAPNFSLPDTEGRIVRLSDFRGAPALLVAF
                     MCNHCPYVKHVADHLAALAKEYQAKGVAVVGINSNDVENYPEDSPEKMREEVALRGYT
                     FPYLFDETQEVAKRYGAACTPDFFLFDKEQRLVYRGQLDDSRPNNGIPVTGKDLRAAL
                     DAVLAGQPVSPDQKPSLGCNIKWKPGNAPDYFGK"
BASE COUNT          745 a          937 c          979 g          701 t
ORIGIN      
        1 ggggacccaa gcccagttgt ttccgtaaat ccaaacgttc acgcaatcga cgggaccacg
       61 aaacgcgaca gcttgtgggg gacggatgat gacaacgggt tttggcccga cacctcggta
      121 ggttaacttg cccacgtatt tgccccagat ttgctgctcg cgtgaacgac gaaaatcagc
      181 cactgcatca cgggcttcca atgtccagcc ctccaagtcc tcaaagtcga tcaatggtgg
      241 ccgggtgtcc tcaaaacgat tggcccaatc catctcgtaa ggacgtttcc ccactacttc
      301 ttcctcttcg gcagttgctc tgatcgaacc gagaaggccc cctccggcga ccaggcatgc
      361 caacagcaat cgaatacgat ccgaggtgaa catggcgctg ctcccgaacg caaaggccaa
      421 tttcatcttc gcgggcatgc ttttactggt gggacgactt gctaaagcac caccggccaa
      481 gcacaatggc ttccggccag cccccattat ggcaatgcca ctgggtgttg gggagaggct
      541 ttacacggtc aagtatgagg agacactgtc atgcgacatc attggagacg aaatctcagc
      601 gtgatgatca tcgtgatcat gtccaactta gctgtctttt tcttgggagc cagaactgcg
      661 atcgcccaac aggcatccta cccggagcgc tgggtatggg tcttcggctg gggactaggc
      721 cgcgattcgg acgtgacaga ggtgctgaac ctcctggatc gcgcggggcg aagcggctac
      781 accggggcgg cggtttcctt tggcctggat tccctttgca agtgggacga cgcgcgtttc
      841 cggcggctgg aagttgtgaa ggaagcctgt gcccgcaatc acctggagct tatcccctcg
      901 atcttttccg tgggctatgg tggcggcatt cttggtcaca atcgttattt ggcggagggg
      961 cttcccgtca aggatgcttt atttgaggta cacgggcaag aagcgacact catccccgat
     1021 ccacccgtgc aaattcaaaa tggcggtttc gaagagtatt ctggacagcg cttcaccggc
     1081 tggcgttttc acgatcaacc gggggaagtg agcttcgtcg atacacaggt caagcacagt
     1141 ggccaagcgt cgcttcgcat ggagaacttt cgggcgaatc aacatggcca cggtcgcgtc
     1201 atgcaggagg tcaaggtcca tccctatcgg tgttacaaga tgacgctttg ggtaaaaacg
     1261 gaggacctcc aaccgaccag tgcgtttatg gtccaggtgc tggcagacaa ccgtgcgttg
     1321 gcccccattg gatttaatct ctcgccaacc atggactgga cgaagctcgt cttggtcttc
     1381 aatagccatc gtttcgaggt ggttcgagtt tacgcagggg tctggggcgg acgaagtggc
     1441 aagttctgga tcgacgattg gtccatcgaa gagatgggcc ccatcaacgt gatccggcgg
     1501 cccggtacac cggtctcggt caagagcgaa gacggctcgg ttgtgtacga agaaggtcga
     1561 gattatcagc gactcgaaga ccccaattac agcccatatc gggtggaccg cccggcgcca
     1621 ccgttgcggt tgcttcccgg aagtcgcatc aaagatgggc aacgactccg cgtgagttgg
     1681 tatcacgccc agaagattca cgaatctcag gtcacggtgt gcatggcaga accggaattg
     1741 tacgaaatct tcgatcacga agctgcactg ctcgcaaaac atgtcaaccc gaaaaaggtc
     1801 atcctcagca tggacgagat ccgcatgggt gggacctgtg aggcctgtcg tggtcgggac
     1861 atgggccagc ttttgggcga atgcatcaca aagcaggtgc aaatcctgcg gaaatacatg
     1921 cccgatttgg aggtttacat ctggtcagac atgctagacc ccaaccacaa tgcccacggc
     1981 gattactacc tcgtcgaggg cgacttcacg gggagctgga accacgtgcc gaaggacctg
     2041 gtcatcgccg tttggggtgg cgagccgcga cccaagagcg tgcaattctt cgccgaacag
     2101 gggttctcgc aactgatcgc ctgctactac gacgccccaa acctggaagg tgtgaaaggt
     2161 tggctaaacg tcgtccaggg cgtaccgaaa gtgaaaggct tcatgtacac cccgtggaga
     2221 aaaagctacg atctgctcga ggaattcgga cggctagttt cggggaagca atagacaaag
     2281 acagggcgga cgcgacagaa tgtgtccccc tagggtagcc gaggcgggga actgcgcgcc
     2341 tccaaaataa cggacgcaga ggaccgcgtc cctcccagac ggtgtccctg gcaaaccaaa
     2401 ctgcggtagt agagggtcat gtcccatcgt ggtcaagcca aaggcccgct aagagcctat
     2461 cccataacct cccctcgccc gctcggcggg agaggggccg ggggtgaggg tgactcgaat
     2521 cgctgctggt tttctcactc gactccgacg atcgcttgcc ctcaccccaa ccctctccca
     2581 gagggagaga gggacttttt gggacgggct cttatttgcc gaaataatcg ggtgcgttgc
     2641 cgggcttcca cttaatgtta caacccaaag agggcttttg atcgggcgac accggttgac
     2701 cggccagtac ggcgtctaag gcggcgcgga ggtctttgcc ggtcactggg atgccgttgt
     2761 tcggtcggct atcgtcaagc tgtccccgat agacgagccg ttgctctttg tcgaaaagga
     2821 aaaagtccgg cgtgcaggcc gccccataac gcttggccac ctcttgggtc tcgtcgaaca
     2881 gataggggaa ggtgtaaccc cgcagcgcga cctcctcccg catcttttcg ggcgagtcct
     2941 ccgggtagtt ttccacgtcg ttcgagttta tgccaacaac ggccacgcct ttggcttggt
     3001 attccttggc cagtgcggcc aagtgatcgg ccacgtgttt gacataggga cagtgattgc
     3061 acatgaaggc aacgagcaag gccggcgctc cacgaaagtc ggaaagccga acgatccggc
     3121 cttctgtgtc gggcaaagaa aaattgggag caggagcacc caagggaagc atcatcgtgc
     3181 tggcagtgcg aaccatcgta tgttcctccg ttttaatcga ctacggactt gaactcttcg
     3241 ttttgcagag ggtagaggcg tcaaacgccc caacaacctt gctttgccaa ttctacactg
     3301 ccaggaaaga gctaccttct aaacgccgag ccggttttca acagcctgcg ttgatgcgct
     3361 cg
//
LOCUS       DAOPTR010000364         3361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22577_length_3361_cov_2.722626, whole genome shotgun sequence.
ACCESSION   DAOPTR010000364 DAOPTR010000000
VERSION     DAOPTR010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22577_length_3361_cov_2.722626"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(254..853)
                     /locus_tag="PKI05_07915"
     CDS             complement(254..853)
                     /locus_tag="PKI05_07915"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOA52168.1"
                     /translation="MKRTIILASQSPRRRQLLEEAGYSFVVIPPDDLAECGICSGESP
                     PQFAARLAYQKAANVASRIREGLILACDTVVSCDGQILGKPRDEDDARKMLHLLRGRE
                     HSVFTGVCLWDYPHSSPKIQVAETRLVMAELTDEEIEEYLASGLWEGKAGAFGYQDRL
                     GWLRIIEGTESNVVGLPLECLERMLREYQQPRATDSSGL"
     gene            1190..2644
                     /locus_tag="PKI05_07920"
     CDS             1190..2644
                     /locus_tag="PKI05_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOA52169.1"
                     /translation="MRAALYCCFAVVLTAVIGGGPIYGGNLAAKPNFVVINIDDLGYA
                     DIGPFGSKINRTPNLDRMAAEGRKLTSFYAAPVCSPSRAALMTGSYAKRALPIPHVLF
                     PANDVGLSPDEVTLAEVLKTAGYTTALIGKWHLGDQPPFLPCQQGFDYYFGLPYSNDM
                     GPAEDGVKSSLGEPLPQRKPGQVGQPPLPLMRNNTVIKRVLPDDQQALVEMYTEEAVG
                     FIKRNADKPFFLYLAHNAVHFPIYPGKRWAGRSPHGIYSDWVEEVDWSVGQVLDMIRS
                     LGLSEKTLVLFTSDNGGTQRAVNRPLRGFKGSTWEGGVRVPTIVWWPGKIPAGTECDA
                     IMGMFDVLPTFAYLAGAPLPQDRKLDGMNVWDHLTGAAGDRPAHEVFYYYRGLKLEAV
                     RYLQWKYELPGVRGKEQKAKLYDLKADIGETTDVAASHPEVVKKIESLIQAAESDLGL
                     DSMGPGCRPLGRVEDAQPLIGYDGKVRPGFEPKE"
     gene            2723..>3361
                     /locus_tag="PKI05_07925"
     CDS             2723..>3361
                     /locus_tag="PKI05_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52170.1"
                     /translation="MLRAVAGIVCIGLLTMGQMTQGWAKETVLYVAPNGNDSWSGRIS
                     QPNPAGNDGPLATLTGARNAIRRLKGEGQTGPFRVLIASGRYSLKEPLVLEPQDSGTS
                     ESPIVYEAASKEKPRFSGGRQITGRRKYDDRLWVTTVPDVADGKWYFEQLWVNGRRAT
                     RARSPNKFYYYVQKKIDVYQDADSGKIVETVNRAFQARPEDIKPLLTLTRQQL"
BASE COUNT          765 a          860 c          970 g          766 t
ORIGIN      
        1 ccctgggctg gccggtttag tgccgattgc agctgagagc ccatcccgaa aagtccccct
       61 ctccctccgg gagagggttg gggtgagggc aagtgatcgt cggagtcgat cgagaacacc
      121 ggcagcgatt cgatccaacc ccaaccccgg acccctctcc cgccgaacgc gtgaagggag
      181 attatgggat aagcgctaag cgatataaag ggttggtttt tattgcaata attccccggt
      241 ttggccaacg gcgttaaaga ccgctcgaat cggttgcccg gggctgttgg tattctcgca
      301 acatcctttc taaacactcc agtggtaggc caacgacgtt gctttcggtg ccttcgataa
      361 ttcgcagcca gcccaaacga tcttgatagc cgaaagctcc cgctttgcct tcccaaaggc
      421 cggaagccag gtactcctcg atctcctcgt cggttaactc ggccatgacg agccgtgttt
      481 cagcgacttg gatcttgggc gaagaatgtg ggtaatccca caggcaaaca ccggtaaaga
      541 ccgaatgttc tcttccgcgt agcaaatgaa gcatcttccg cgcgtcatct tcatctcgcg
      601 gtttacccag gatttgcccg tcacaactta cgacggtgtc acaagcaagg atcagtccct
      661 cccggatgcg agaagcaaca tttgcggcct tttgataggc caagcgtgcg gcgaattgtg
      721 gcggactttc gccgctacaa atcccgcact cagccaagtc gtccggaggt atcaccacaa
      781 aagaataacc tgcctcctcc agcaactgac gccgccgcgg tgattggctt gcgaggataa
      841 tggtccgttt caaaggagac gtcatgcctt ttaactggta gtaagtggtt aagacaggct
      901 cttactcggc aactcttcaa tttacaatag tatgccacgt ttggagggcc ggccagcatc
      961 ctcgagagat tgacaagcct tcatggcacc accccgggcc gttgctaacg cggtgtgcct
     1021 tggcgacaat gtaatacacc aaacgagatg tgtttcctcc aaaaaccatc tcgccaactt
     1081 gggtaccctg cgtagtgacc gtttccatgg ttggccgcgc cgtggcggtc atgacttgga
     1141 aaccgtctcc ggttttgatg ttttagtgct atttggggag gataacacca tgagagctgc
     1201 cctgtattgt tgttttgctg ttgtgttaac tgcggtcatc ggaggtgggc ccatttacgg
     1261 cggcaatctc gcggcgaaac ccaacttcgt cgtgatcaac attgacgacc tggggtatgc
     1321 tgacattggt ccgtttggat caaaaatcaa tcgaacgccg aacctcgatc ggatggcagc
     1381 ggaagggcgc aaattgactt cgttttacgc tgcgccggtg tgttctcctt cacgtgctgc
     1441 gctcatgacg gggagttatg ccaagcgggc actaccgatt ccgcatgtct tgtttcctgc
     1501 caacgacgta ggactgtctc ccgatgaggt aacgttggcg gaagtcttga aaacagcggg
     1561 atacacaacg gccctgatcg gcaaatggca tctcggtgac cagccaccat ttttgccgtg
     1621 tcaacaaggt ttcgattatt acttcggttt gccttactca aacgatatgg gccctgctga
     1681 agatggtgtc aagagtagtt tgggagaacc gcttcctcaa aggaaaccgg ggcaagtagg
     1741 tcagccgcca ttgccgctga tgcgcaataa tacggtcatc aaacgggtcc tgccggatga
     1801 ccaacaggcc ctagttgaga tgtacactga ggaagcagtc ggttttatca agaggaatgc
     1861 ggacaagccg tttttccttt acttagccca caatgcggtg cattttccca tttatcccgg
     1921 gaagagatgg gccggacggt ccccgcatgg gatttattcc gactgggtgg aagaggtgga
     1981 ttggagtgtg ggccaggtgc tcgacatgat tcgctcgctt ggactgagcg aaaagacttt
     2041 ggtgctgttt acgagtgaca acggcgggac acagcgagcg gtgaacagac cgttaagagg
     2101 gttcaagggc agcacgtggg aaggtggcgt tcgtgttccc accatcgtgt ggtggccagg
     2161 caaaattcct gcgggaaccg aatgtgatgc gatcatgggg atgttcgacg ttttgcctac
     2221 gttcgcgtac ctggctggtg cccctcttcc ccaggatcga aagctcgacg gcatgaacgt
     2281 gtgggaccac ttgacgggcg ctgccggtga tcgtcccgcc catgaggtgt tttactacta
     2341 ccgaggtttg aagctggagg ccgttcgcta cttgcaatgg aaatatgagc taccaggggt
     2401 acggggcaag gagcaaaagg cgaagcttta tgacctgaag gccgatattg gcgaaaccac
     2461 ggacgttgcc gcgtcgcatc cggaagtcgt taagaagatt gaatcgctga ttcaagccgc
     2521 cgagagcgac cttggccttg atagcatggg acccggctgc cgtcctcttg ggcgggtgga
     2581 agatgcccag cctttgatcg gttacgatgg aaaagttcgg cccggtttcg agcccaagga
     2641 atgagcatct gcgccaaccg gcagatggat cccgcagttg tgcaagactg tttgacatcg
     2701 cggagttgga ggaacacgtg ccatgttacg ggctgtggct ggaattgtgt gcattggcct
     2761 gttgacaatg ggtcaaatga cccagggatg ggccaaagaa acagtactat atgttgcgcc
     2821 caacggaaac gattcctggt cggggcggat aagtcagccc aatccagcag gtaacgatgg
     2881 tccgttggcg actttgaccg gcgcgagaaa cgcgatccgt cgtttgaaag gagaagggca
     2941 aacgggtcca tttcgtgtct tgattgcttc gggtcggtac tccctaaagg aaccgttggt
     3001 tcttgagccc caagattctg gcacgagcga gtcgcccatc gtctatgagg cggcaagcaa
     3061 ggaaaagccg aggttttcag gaggcaggca gataacgggt cggaggaaat atgacgacag
     3121 gctttgggtc acgaccgtcc ccgatgtggc tgacggcaaa tggtactttg agcaactttg
     3181 ggttaacggc agacgagcga cccgtgctcg aagccccaac aagttctatt actatgtgca
     3241 aaaaaagatc gatgtctatc aggacgccga ttcaggtaag attgttgaaa ccgttaaccg
     3301 ggcgtttcag gcaagaccgg aagatatcaa gccacttttg accctcactc gtcaacaact
     3361 t
//
LOCUS       DAOPTR010000365         3356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22616_length_3356_cov_3.127840, whole genome shotgun sequence.
ACCESSION   DAOPTR010000365 DAOPTR010000000
VERSION     DAOPTR010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22616_length_3356_cov_3.127840"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PKI05_07930"
     CDS             <1..406
                     /locus_tag="PKI05_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HOA52171.1"
                     /translation="AAVEEGILPGGGVALLRAAMQCKPDGLTTDEKVGYEIVLRACRS
                     PIMQIASNAGQDGSIVCEKVSEMKGNMGFNALTGQYEDLVKAGVIDPTKVVRVALQNA
                     ASVATLMLTSDALVAEKPKEEKSGGGHTPPEY"
     gene            774..2672
                     /locus_tag="PKI05_07935"
     CDS             774..2672
                     /locus_tag="PKI05_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52172.1"
                     /translation="MQPPLECRPLQIVHGLTPQRVVADSVAQILQQPTAQPISDEGMA
                     YYLRRGRGGIVCNVAFQDYLRSEANWQTLIEGVDLCARLKMPVWIYDEKGYPSGAAGG
                     LVLEGNPEFEALVLAWEPRQTPAFFLRRAFEHTHAANNYHAVRRYINLMDDRATAKFI
                     QVTHEAYYKRLARHFGSTIVAFFTDEPSLMAVDLGAIPEPARSRVPIEDAPDPNVKPL
                     PTVPWCYDLENVYRSKWGEDLRAQQKSLFVGNDPEDRRIRRQFWSIVAELVADRYFGA
                     LRRWCQGHNVASSGHNLHEESILHHVPLYGNGLKAIAQMDVPGLDMLSSNPEVVIHSG
                     WLTAAMPVSGALLTGCRRVMTEVSDFSQKMSGAGPVGLPEMMATAAWQAAWGVTEFTL
                     YYNPEDRSVEENWQYGQFVGRLNAILREAMPRRRVALYYPIYDLWAEYLPVAGPLHQS
                     VRSEKVQQLIGSFMRLGQTMQRRQIPFVLIDHEYLQRSRVSSDGRLCVGNLELESVVM
                     PAGVELPHEAQAVIEQFQQAGGRVIRDATDAPLTAAKLVEAIRPLYHIEPANDRIVFG
                     EFEREGRTILILANVSREPYKGVLRAKQHKTWTALDPITGEIRAVTATEQGIPVHLDG
                     YKAILISQ"
     gene            complement(2808..3203)
                     /locus_tag="PKI05_07940"
     CDS             complement(2808..3203)
                     /locus_tag="PKI05_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOA52173.1"
                     /translation="MRHQHGAKRMAQAQPEYLRGDRYYSPPVDILEKGDEILLIADVP
                     GTKSEDIDIQFEDGMLTVHAKVNPRYPEGVEFLQQEYGVGDYLRTFQVSEAIDASKIS
                     AEYENGVLTLHLPKAASLRRRKIEVTAKG"
     gene            complement(3251..3343)
                     /locus_tag="PKI05_07945"
     CDS             complement(3251..3343)
                     /locus_tag="PKI05_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HOA52174.1"
                     /translation="MQADLQNGVLTITLPKAETVRPRKIQVNVR"
BASE COUNT          720 a          913 c          950 g          773 t
ORIGIN      
        1 agcggccgtc gaggaaggca tcctcccagg cggtggagtc gcgctcctca gggctgctat
       61 gcagtgcaaa cccgatgggt tgaccaccga tgaaaaggtg ggttatgaaa tcgtcctgcg
      121 ggcatgccgc tcaccgatca tgcagattgc gtccaacgct ggccaggacg gtagcatcgt
      181 ctgcgagaaa gtcagtgaaa tgaagggcaa catgggcttc aacgctttga cgggtcagta
      241 cgaagacctc gtcaaggccg gtgtcatcga cccgaccaag gtggttcggg tggccctgca
      301 aaacgccgcc agtgtggcaa ctctaatgtt gaccagtgat gccctggtgg ccgaaaagcc
      361 caaggaagaa aaatccggtg gtgggcacac accgcctgag tattaatcca ctgcccctta
      421 acgcgtgctg ctcaactcgg atgggcagca cgcaatcgct gaagtcgccc ccttcccctc
      481 ccttcgagag cgtccccaac cccgctgggg acgctcgttt ttttcttcgg gagcttcaag
      541 gcacgttcct tcttacctaa aatagcggca tgttgtcgca acggcttttt caccacagtg
      601 gctttatttc cggagatcac ttcatgagga cgagcatcca aggagatcga tgggcgagga
      661 agactcgagt acgactcttg accgttttct cggtcatcac ctgcctcggc tgcggagcgg
      721 ccgctcttgg cgcggaggat tcgcctttgc caaagtcttg gatggaggca tggatgcaac
      781 caccactgga atgccgtccg ctacaaattg ttcatggctt aacaccccag cgcgtggtgg
      841 ccgacagcgt cgcacagatt ctgcaacagc ccacggcgca gccaatatcg gatgaaggga
      901 tggcctatta ccttcggcga ggccgcggag gaattgtgtg caacgtggcc tttcaggatt
      961 accttcggtc ggaggccaac tggcagacgt tgatcgaagg cgttgatctt tgtgcccgtt
     1021 tgaagatgcc tgtttggatc tacgacgaaa agggttaccc aagtggtgcg gcgggaggac
     1081 tggttctcga aggcaatccc gagtttgaag cccttgtctt ggcgtgggaa ccgcggcaga
     1141 cgcccgcgtt tttcctccgc cgggcattcg aacataccca tgccgcgaat aactatcatg
     1201 cggttcgccg ctatatcaac ctcatggatg atcgtgccac cgctaagttt attcaggtga
     1261 cgcacgaagc ctattacaag cgattggccc gccatttcgg ttcaaccatt gtggcctttt
     1321 ttacggatga accttccctc atggccgttg acctgggggc aattccggag cctgcgcgca
     1381 gtcgagttcc catagaggat gcacccgacc ctaacgtaaa gcccttaccg accgtgccgt
     1441 ggtgttatga tctagaaaac gtttatcgga gcaagtgggg tgaagacctt cgggcccaac
     1501 aaaagagcct ttttgtcggt aatgatccag aagatcgtcg catccgccgc cagttttggt
     1561 cgattgttgc agagttagtt gcggaccgct actttggcgc tcttcgccgg tggtgtcaag
     1621 gccataacgt cgcctcgtca ggccacaatt tgcatgagga atcgatcctc catcatgtcc
     1681 ccctttatgg gaatggtctg aaggccatcg cacaaatgga tgtccccggg ctggacatgt
     1741 taagctcgaa tcctgaggtc gtcattcaca gtggatggct gacggcggcc atgccggtat
     1801 ctggggccct gttgacagga tgccgtcgcg tgatgacgga agtcagcgat ttttctcaga
     1861 agatgtcagg ggcgggaccg gtagggcttc ccgaaatgat ggcaacagcg gcatggcaag
     1921 cggcgtgggg tgtcacggag tttacgcttt actataaccc ggaggaccgc tccgtggagg
     1981 agaattggca gtacggccaa ttcgtggggc gccttaatgc gatcctccgc gaggccatgc
     2041 cccggcgcag ggttgctctt tactacccca tttatgacct gtgggccgag tatcttcccg
     2101 tggcaggtcc tctccaccag tcggttcgca gtgagaaagt ccagcaactg attggctctt
     2161 ttatgcgcct cggacagacc atgcagcggc ggcagattcc gttcgttttg attgaccatg
     2221 aatacctaca gcgatcacgg gtaagttccg acggccgcct ctgtgtgggc aatctggaac
     2281 tggaaagcgt tgtcatgccg gccggggtgg agctgcccca tgaggcgcaa gctgtgattg
     2341 agcaattcca acaagcaggt ggccgcgtca tccgagatgc tacggatgcc ccgctaacgg
     2401 cagctaagtt agtcgaggcc atccggccct tatatcacat agaaccggca aatgatcgga
     2461 tcgtctttgg agagtttgag agggaagggc gaacgatttt gatccttgcc aatgtttcgc
     2521 gggaacccta caaaggtgtt ctgcgagcaa aacaacacaa gacctggacg gcgttggatc
     2581 cgattacagg tgaaatacgg gcagtcaccg cgacagagca ggggatcccc gttcaccttg
     2641 atggctacaa ggccatcctg ataagtcaat aaggcaattt cttggcatat gcttcccgtc
     2701 acgcccgtca aaagggaaaa ccgaggtggc gagccaacct cggtttgtct ttcgttgatc
     2761 tatgttgtga caacgccccg aaccggccgt caaatttatt gagtttatta ccctttcgcg
     2821 gtgacctcaa tctttcgacg acggaggcta gccgccttgg ggaggtgtag ggtcaagacg
     2881 ccattctcat attcagccga tatcttggag gcgtcgattg cctcgctgac ctggaaggtc
     2941 cgcaagtaat ccccaacacc gtactcctgt tgcaagaact caactccttc agggtagcgt
     3001 gggttgacct tggcgtggac ggtcaacata ccgtcttcga actgaatatc aatgtcttct
     3061 gacttggttc cggggacatc cgcaattagc aggatttcat cccctttttc caaaatgtca
     3121 acaggtgggc tgtaatatcg gtcaccacgc aaatattcgg gctgggcctg ggccatacgt
     3181 tttgcaccat gttggtgtct catcgctatc tctccttctc actaagcgat tgacacgaca
     3241 tccaccggcg ttatcgcaca ttgacctgga ttttgcgcgg ccgaacagtt tccgcctttg
     3301 gcaaagtgat ggtcaacacg ccattttgga gatcggcctg aatgcgatcg acatcg
//
LOCUS       DAOPTR010000366         3355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22628_length_3355_cov_3.317273, whole genome shotgun sequence.
ACCESSION   DAOPTR010000366 DAOPTR010000000
VERSION     DAOPTR010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22628_length_3355_cov_3.317273"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PKI05_07950"
     CDS             <1..241
                     /locus_tag="PKI05_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOA52175.1"
                     /translation="DTYPYIELEEQDVMIGHEASVSKIGEEQLFYLCSRGLTEAEAST
                     MIVAGFIEPLVKELPMEYAVEMNRLIALQMEGSVG"
     gene            398..1720
                     /gene="sufD"
                     /locus_tag="PKI05_07955"
     CDS             398..1720
                     /gene="sufD"
                     /locus_tag="PKI05_07955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOA52176.1"
                     /translation="MMMPSLNLDTSFTDEGFHNVAFHRPQPNWLKELRQRAYRTFEDL
                     PMPNRSMEEWRRTELRHFRLLQFQAWSPHPEQAPEIPSQLIPRLDTDGLIITLNGRKL
                     ESRLDPALGQQGVVLNCLEDSILNGWEELIRKHLFSILDWKYDKFAAMHAAFWTGGIF
                     VYVPKDIRVNKPLLHVAALMPGQADVGHTLIILDEGAEATVVSETVSVDPSASSLYCG
                     GIEIILRPGARLRYVNMQNWNQATFHFAHQKAVLAERASLEWTLGALGGRLAKVNQHL
                     VLDGPESEGIVNGVMFAENRQHLAYHTLQHHRTPKTKSDLLYKGALQDQSHLVWRGMI
                     RVEPGAVKADGYQRNDNLMLSETARTDCIPGLEILADDVRCTHGATAGRVDEEEIFYC
                     QTRGLTRREATRLVVTGFFQQVLDRITIDSVREALTDIIQHRVREYRG"
     gene            1770..2090
                     /locus_tag="PKI05_07960"
     CDS             1770..2090
                     /locus_tag="PKI05_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HOA52177.1"
                     /translation="MGELIRVCRVSDLPDPGKAVFDVSGRMIALFHVSGTFWALDDMC
                     THDGGPLAEGELHGYTIVCPRHGAQFDIRTGQAKTMPAVRATTAHKVVVKGDEVYVEL
                     NEES"
     gene            2127..2444
                     /locus_tag="PKI05_07965"
     CDS             2127..2444
                     /locus_tag="PKI05_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HOA52178.1"
                     /translation="MAIREETVWEALKQVRDPEIGIDLVNLGLIYEILITEHEGKSDI
                     HVKMTLTSPACPSGPQMIQEVKDVLRQLGDEVGQVNVEVVLSPPWTPDRMSEEARDEL
                     GFY"
     gene            complement(2507..>3355)
                     /locus_tag="PKI05_07970"
     CDS             complement(2507..>3355)
                     /locus_tag="PKI05_07970"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOA52179.1"
                     /translation="NWLGAKIDEEDFQKNRYDVVTRCIERGINYIDACTGQEVMAYAR
                     ALKGRRDKMYLGYSWYQRESRFAEWRSVEKLKESLDMGLKEAGLEYVDLWRITLHEQS
                     SRHSEAEIEAVIAALDWAKKTGRARFTGISSHDRPHIKMLIEKYPEQLEVICTPYTAK
                     TKVVQDETGLWAAMQKHDVGWFGIKPFASGSLFKGDSSPNSPYREEDDRLARLAIRYI
                     LCNPAITAPIPGMITPEQVDNVALAVKERRELDLEEKALLDREMEQAWANLPYHYQWL
                     KDWEYV"
BASE COUNT          739 a          885 c          963 g          768 t
ORIGIN      
        1 cgatacgtat ccttatattg aacttgagga acaagatgtg atgattgggc acgaggcaag
       61 tgtgtccaag atcggggaag agcagctctt ttatctgtgc agccgtggcc tgacggaggc
      121 cgaggccagc acaatgatcg ttgcggggtt catcgagccc ttggtcaagg aattgcccat
      181 ggagtacgcc gtcgaaatga accggttgat tgccctccag atggaaggga gtgtcggctg
      241 atcgtcttac ggcgacgttt ggagtaatcc ctcacggggc agtgtgggaa cgcgatgtgg
      301 cagaggggac acgggtgttt ggcggaagca gccaagtgtt acaaataact ttccaaaaga
      361 atgggttcta tcccagcagg accgtttagt caaagcgatg atgatgccat ctcttaacct
      421 tgacaccagc ttcaccgacg aaggattcca caacgttgcg ttccatcgac cacaacccaa
      481 ctggctcaaa gagcttcggc agcgggccta tcgcacgttt gaagatttgc cgatgccgaa
      541 tcgctccatg gaggaatggc gacgaacgga actgcggcac tttcgcctgc ttcaatttca
      601 agcgtggtct ccccatccgg agcaagctcc tgagattcct tcccaactta tacctcgact
      661 tgatacggac gggttgatca tcacgctcaa tgggcgaaag cttgaaagcc ggctcgatcc
      721 cgcgcttggg caacagggtg ttgtgctgaa ttgtctcgag gactcgattc ttaacggttg
      781 ggaggagttg atccgtaaac acctcttctc catcctggat tggaaatacg acaagtttgc
      841 cgccatgcat gctgcctttt ggacaggcgg tatattcgtc tatgtgccca aagacataag
      901 ggtgaataaa cctctcctgc atgtcgcagc cctgatgcct ggtcaagctg atgtgggaca
      961 cacactgatc atcctggatg agggcgctga agccacggtg gtcagcgaga cggtcagtgt
     1021 cgatccctcg gcaagtagcc tatattgcgg cggaattgag atcatcctcc gtccgggggc
     1081 acggttacgc tacgtcaaca tgcaaaattg gaatcaggca accttccact ttgcgcatca
     1141 aaaggccgta ctggcggagc gggcctcgct ggagtggacc ctaggtgccc ttggtgggcg
     1201 attggccaag gttaatcagc atctcgtcct ggatggtccc gaaagtgaag gcatcgtcaa
     1261 tggcgtgatg tttgcggaga atcgccaaca tttggcatat catacgctgc aacatcaccg
     1321 gacgccgaag actaagagtg atctccttta caagggcgcc ctccaagatc agtcccatct
     1381 cgtttggcgg ggcatgattc gcgtggaacc aggggctgtg aaagccgacg gttaccaacg
     1441 gaacgacaat ttgatgctca gcgagaccgc gaggacggat tgcatccccg gattggagat
     1501 tttggccgac gatgtgcgat gcacacacgg ggcgacagcc ggtcgcgtgg atgaagagga
     1561 gattttctat tgccaaacgc gcggtcttac tcggcgtgag gccacccggt tggtcgtcac
     1621 cggcttcttc cagcaggtct tggatcggat caccatcgac agtgtccggg aagccctgac
     1681 ggatatcatc cagcatcgtg tgcgggaata ccggggctaa gccgtaaaat ccaatactgt
     1741 ggcgctcggt ggcagaaaag gaagacaaaa tgggcgagtt gattcgtgtt tgtcgtgttt
     1801 cggacttgcc tgacccggga aaggccgttt tcgatgtaag tggtcgaatg atcgccctgt
     1861 tccatgtttc cgggacattt tgggcccttg atgatatgtg cacgcacgac ggtggaccac
     1921 tggcggaagg cgaacttcac ggttatacga ttgtttgtcc tcgccatggg gcgcagtttg
     1981 atattcgcac cggccaggcc aaaacgatgc cggccgtcag ggccacgact gcccataagg
     2041 ttgtcgtcaa aggcgatgag gtgtatgtcg agctgaacga ggagagttga aaggctggct
     2101 tgcaagggga atctggagaa ccttccgtgg ctattcgaga agagaccgtt tgggaagcac
     2161 tcaaacaggt tcgtgatccg gaaattggga tcgacctggt aaacctgggg ttgatctacg
     2221 agatcttgat cacagagcac gagggcaagt cggatatcca cgtaaaaatg accttgacca
     2281 gtcccgcgtg tccctctggt ccacagatga ttcaggaagt gaaagacgtc ctccgccaac
     2341 tcggggacga ggtcggccag gtcaacgtcg aggtagtctt atcgccgcca tggacgccgg
     2401 accgcatgtc tgaagaagcc cgtgacgagc tgggcttcta ttgacatgcc agatcgcggc
     2461 aatggcctgc ggttacgaaa tgacaggcct tgaaacagcc ggcgtattac acatattccc
     2521 agtctttaag ccactgatag tgatagggca ggttcgccca ggcctgttcc atttcgcggt
     2581 cgagaagtgc cttctcttcc aggtctagct cgcgccgttc cttgacggcc aatgctacat
     2641 tatcgacttg ttccggcgtg atcatgcctg ggatcggtgc cgtgatcgcc ggattgcaca
     2701 agatatagcg aatcgccagc cgggccaggc ggtcgtcctc ctcgcggtaa ggactgttgg
     2761 gcgagctgtc gcccttgaac agcgagccgc tggcgaaagg cttaatccca aaccacccca
     2821 catcgtgctt ttgcatcgcg gcccacagcc ctgtctcatc ttgaacgacc tttgtcttgg
     2881 ccgtgtaggg cgtgcagatt acttccaatt gttcgggata cttttcgatc agcattttga
     2941 tgtgcgggcg gtcgtgagac gatatgccgg tgaaacgtgc ccggcctgtc tttttggccc
     3001 aatcaagggc ggcaatgacg gcctcgatct cggcttcact gtgccggctg ctttgttcgt
     3061 ggagggtgat acgccacaaa tcgacgtact ccaagcccgc ctccttcagt cccatatcca
     3121 ggctttcctt aagtttctcg acacttcgcc attcggcgaa tcgggattcc cgctgatacc
     3181 aggaatagcc gaggtacatc ttatcccgac gacctttgag agcacgggca taggccatca
     3241 cctcttgccc tgtgcatgcg tcaatgtaat tgatcccgcg ctcgatacaa cgggtcacca
     3301 catcataacg gttcttttgg aaatcctctt cgtcgatctt ggcccccaac cagtt
//
LOCUS       DAOPTR010000367         3353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22646_length_3353_cov_3.614008, whole genome shotgun sequence.
ACCESSION   DAOPTR010000367 DAOPTR010000000
VERSION     DAOPTR010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22646_length_3353_cov_3.614008"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..1879)
                     /locus_tag="PKI05_07975"
     CDS             complement(62..1879)
                     /locus_tag="PKI05_07975"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007332958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOA52180.1"
                     /translation="MRYLSFSSRLGRSLKMMVGSLAVLLIGLVGFSLGDPHPEAGPNR
                     AEGKWASPSPVQQVSISSVTAKTQAMLTAQVRSRSQRPNIVIILADDMGYSDLGCYGG
                     EIETPHLDQLAAHGLRFTQCYNTARCWPSRAAIMTGYYAQQVRRDTVPGVPSGGRGTR
                     PAWAPLLSEILKQHGYRCYHSGKWHIDSQPWNTGFDHGYVVNDHDRFFGPKNHAEDGK
                     PLPQPKPEDNFYLTTEIARFAIKYLKEHAQQHGEQPFFLHLCFTSPHFPLQAPQDVIA
                     HYMDRYRVGWNVIQEERGKRLLQMGLVKNRPPAMEIEVGPPYHFPEALKILGPYEVNR
                     PFPWNQLTQEQQAFQVAKMAIHAAMVHIMDQAIGEVLEQLKAMKALDNTLIMFASDNG
                     ASAEIMVRGDGHDPKAPPGSAQTFLCLGPGWSSASNTPFRRHKTWVHEGGISTPLIVH
                     WPAGIKSQGELRHTPIHFIDVVPTVLDILGISLPQEWNGQPRPPFPGVSFAPLFVQDG
                     SVSHDYLWFLHEGNRAIRVGDWKLVAAHDEPWELYNLAEDRGETENLIQKEPEKAKQL
                     EELWNRLWEQFQADARRDIPADQEKPSPAQKTPRKSAGQ"
     gene            2138..>3353
                     /locus_tag="PKI05_07980"
     CDS             2138..>3353
                     /locus_tag="PKI05_07980"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA52181.1"
                     /translation="MKNAQRNDRTGKLGRREFLQTSSVLGGGILLASPLARSVHAAGS
                     DIIKIALIGAGGRGTGAASQLLNADKGVKLIAVADAFENKATSAAQRLKKQFPDQVDV
                     PPDRIFTGLLCHQGALAADPDLVVLATPPGFRPMQYRAAIEAGKHVFMEKPCCVDAPG
                     YRSLMETNKLADEKGLFVAVGLQRRHEPRYIETIKQLQSGAIGKIHFIRVYWNGSGIW
                     NRPREPGMTEMQYQVHNWYHFCWLSGDNIVEQHIHNLDVANWVLGDQHPVEANGMGGC
                     TARYLGEFKGTGQIFDHHFVEFTYADGTKVFSQCRHMKNCFNAVTEFVHGEAGIAECS
                     GRIEKGGQTVWKYDGPRPNGHQQEQIDLIAALRAGKRYNEGYIGANSSMTAVLGREAN
                     YSGKVITWDELVA"
BASE COUNT          733 a          950 c          966 g          704 t
ORIGIN      
        1 aacaagcgtg cccaactgca tgcgaactgc tttcccgcta cggacatcga tcgcaaccgg
       61 tctactgacc tgccgatttt cgcggtgttt tctgggccgg cgagggtttc tcttggtccg
      121 cagggatgtc ccgccgggcg tcagcttgaa actgctccca gaggcggttc catagttctt
      181 caagttgctt ggccttttcc ggctcctttt ggatgaggtt ttcggtttct ccccgatctt
      241 cggcgaggtt gtaaagctcc cagggttcgt cgtgggccgc caccaatttc cagtcgccca
      301 cccgaatggc ccgatttccc tcgtgcagga accagagata atcgtgcgag acacttccat
      361 cttgaacaaa gaggggagcg aaacttacac ccggaaacgg cggccgcggc tgtccgttcc
      421 actcctgcgg caagctaatc cccaagatgt ccaaaaccgt gggaacaaca tcaatgaagt
      481 ggatcggcgt gtgacgcaac tcgccctgac tcttgatacc ggcaggccag tggacaatca
      541 gcggcgtgga aatcccccct tcgtgaaccc aggttttgtg acgcctgaac ggcgtgttgc
      601 tagcactcga ccagccgggg ccaaggcaga ggaacgtctg ggcgctgccc ggcggggcct
      661 tgggatcatg gccatcaccc cgcaccataa tctcggcact ggcaccgttg tcggaggcaa
      721 acatgatgag cgtattgtcg agggccttca tcgccttcag ctgctcgagc acctccccaa
      781 tggcctgatc catgatatgg accatggcgg cgtggatcgc cattttggcc acctggaagg
      841 cctgctgctc ttgggtaagc tgattccagg ggaaggggcg attgacctcg tatggcccga
      901 ggatttttaa ggcctccggg aaatggtagg gcggtccgac ttcaatttcc atggcgggcg
      961 gacggttctt gaccaagccc atctgcaaaa gacgctttcc ccgctcctcc tggatgacat
     1021 tccaaccaac acggtatcga tccatgtagt gcgcaattac gtcctgcggc gcctgcaacg
     1081 ggaaatgggg tgatgtaaaa cacaaatgaa gaaagaacgg ctgctcgccg tgctgctggg
     1141 catgttcttt caagtattta atggcaaagc gggcaatctc tgttgtcaag taaaaattgt
     1201 cttccggctt tggctggggc aaaggtttgc catcttcggc atggttcttg ggaccgaaga
     1261 accgatcgtg gtcgttgacc acataaccat gatcaaaacc ggtgttccat ggctggctgt
     1321 cgatgtgcca ttttcccgag tggtaacacc gatatccgtg ttgtttgagg atttcggaaa
     1381 ggagcggtgc ccaggccggc cgggtcccac gtccgccact gggaacgcct ggcacggtgt
     1441 cccgtcgaac ctgctgggcg tagtagcccg tcatgatagc agcccgcgag ggccagcatc
     1501 gggccgtgtt gtaacactgc gtgaagcgca acccgtgggc agccaattga tccaagtgcg
     1561 gcgtttcgat ttcaccgccg tagcaaccca gatcggaata acccatgtca tcggccagga
     1621 taatgacgat attcggtcgc tgcgacctcg agcgcacctg ggccgtcaac atggcctgcg
     1681 tctttgccgt cacagacgag attgaaactt gttgcacggg tgagggggac gcccattttc
     1741 cctcggcacg attgggcccc gcttccggat gcggatcgcc taagctgaag ccgacgagcc
     1801 caatcagtaa tactgccaac gatcccacca tcatcttcaa ggagcggcca agccgagagc
     1861 tgaaactgag atagcgcatc gcgttgcctc ccacaattag gattgtacct tctgaaacgt
     1921 cgggcctttc gagcaggcgt tgcgccggat gtcaatccat gcaactatcc cgcaggtgtc
     1981 ataaccgtct ggcaaccatt ataatgtacc gcggccatac aaccagcgtt ggggcgatga
     2041 ccttggctgc ccgtcgtgcg ccgaagggca tttgcgctat catgttgagg gagatttgca
     2101 gccattccac ctcgatcctc taagaaaggc ccgaactatg aagaacgcac aaagaaacga
     2161 tcgcacaggc aagttgggtc ggcgggaatt cttgcagaca tcgagtgtct tgggtggggg
     2221 aatcctgctc gcttcgccgc ttgcccgcag tgtccacgcg gcgggaagcg acatcatcaa
     2281 aatcgccctg atcggtgccg gtggtcgggg aaccggcgcg gcatcgcaac ttttgaatgc
     2341 tgataagggc gtcaaactca tcgctgtggc cgatgccttc gaaaacaagg cgacaagtgc
     2401 agcacagcga ttgaagaagc agtttcctga tcaggtcgat gtcccaccgg accggatctt
     2461 caccggtttg ctttgccacc agggggcctt ggctgctgat cccgatttgg tcgtcctggc
     2521 tactccgccg ggattccgac ccatgcagta tcgagcggcg atcgaggccg gcaagcatgt
     2581 ctttatggaa aagccttgct gcgtcgatgc accgggttac cgatcgttga tggaaacaaa
     2641 caaattggct gacgaaaaag gccttttcgt ggccgtcggc ctacagcgcc gccatgagcc
     2701 gcgctacatc gagacgatca agcaattaca gtcaggggct atcggcaaga ttcatttcat
     2761 ccgcgtgtac tggaacggca gtggtatttg gaatcgaccg cgcgagccgg gcatgacgga
     2821 aatgcagtac caggtgcaca actggtatca cttctgctgg ttaagcggcg acaacatcgt
     2881 ggaacaacat atccacaatt tggacgtggc caattgggtc ctgggcgacc agcacccagt
     2941 cgaggccaac ggcatgggtg ggtgcaccgc ccgttatctg ggcgaattca aaggcaccgg
     3001 acaaatcttt gaccaccact tcgttgaatt cacctatgcc gacggcacta aggtctttag
     3061 ccagtgccgc cacatgaaga attgcttcaa cgccgtgacg gaatttgtcc acggagaggc
     3121 aggcatcgcc gagtgcagtg gccgaatcga aaaaggtggc caaaccgttt ggaaatacga
     3181 tgggccgcgt cccaacggac atcaacagga gcaaatcgac ctgatcgcag ccctgcgggc
     3241 tggaaaacgc tacaacgagg gctacatcgg ggccaatagt agcatgacgg cggtcctcgg
     3301 acgcgaggcc aattactccg gcaaagtcat cacctgggat gaattggtcg ccc
//
LOCUS       DAOPTR010000368         3353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22647_length_3353_cov_3.476956, whole genome shotgun sequence.
ACCESSION   DAOPTR010000368 DAOPTR010000000
VERSION     DAOPTR010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22647_length_3353_cov_3.476956"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PKI05_07985"
     CDS             complement(<1..358)
                     /locus_tag="PKI05_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOA52182.1"
                     /translation="MDTSLSRRMFLKSQAAVAAGAVAAGVLSAEETPPQGAPLRIGVI
                     GVGGRGTYLLRLLLEQGAEVPAVCDIRPARLEIAGKLIAEMRDGQRPATYGDTPTDYR
                     RMLERDDLEAVLIATPM"
     gene            complement(507..1649)
                     /locus_tag="PKI05_07990"
     CDS             complement(507..1649)
                     /locus_tag="PKI05_07990"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOA52183.1"
                     /translation="MRAIFLLGISFTLLPIFVHAADTPETIVAGLQAHNRALHIKDGW
                     IRDPFIVLAPDGYYYLTGTTALPGDARERNDILNTGLGPQSIVGWKVRLWKSPDLIHW
                     EYCGEPFSLKDGVWAEVQPQRFRQVPESAWRLWAPELHWLGNRWALVHTSPSPVRASN
                     LSLTEGPEIKGPWKNPMGEKIGHRHDPSLFRDDDGTWWLVWGATEIAPLKPDFSDFAQ
                     QPIKIGPTEPDGTLGTMGHEGCFIKKFGRKYVLFGTGWSTRQMRKGSYNLYYATADRI
                     EGPYSVRRFVGRFLGHGTLFQDKAGHWWCTAFYNANVPPLSREEARGKDLSETAQTIN
                     PQGVTIVPIFIKTLPDGDVFIRAKDPDYANPGPDELQQFDYWEPQE"
     gene            1892..>3353
                     /locus_tag="PKI05_07995"
     CDS             1892..>3353
                     /locus_tag="PKI05_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008669321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOA52184.1"
                     /translation="MNFWAMPSMKANKLALSWLAWMLTIVALVATSSATETSQQRRNI
                     LLICVDDLKPVLGCYGDPVAKTPHMDRLAARGMRFELAFCNQAVCAPSRNNLLLGSRS
                     TSIGIYGLSENFRRAVPDAVTMPQYFMKYGWRTEAVGKVFRTGHGNHDDAASWSVPSV
                     IEKVVEYVDPQNSAGGQLTREEAYFTNQMLDRIGSLPRGAAWEKLDVADQAYADGRIA
                     DEGIKRLRAAKERRQKEGTPFFLALGFVKPHLPFTAPKKYWDLYSPTEFKLASYQEPP
                     KGAPPVANKRGGEILNYDPLTMENLKTDEIQRTLIHGYYAATSFADAQIGRVLDELDR
                     LGLAESTIVVLWGDNGFHLGDHGIWTKHTNYEQAVRIPLIIVAPGVTPPGSVTRQVAE
                     NVDIFPTLAELAGLPRPTGPQPIDGVSLVPVLRNPDTRVDDHAYHCFPRGAVMGRAIR
                     TERYRLVEWKKIGAPAETAEYELYDYQTDPEERENLI"
BASE COUNT          763 a          936 c          916 g          738 t
ORIGIN      
        1 gcatgggcgt agcgatgagc accgcctcca agtcatcacg ctctagcata cggcgataat
       61 cggtgggcgt gtcgccataa gtcgctggtc gctgcccatc tcgcatttcg gcgataagtt
      121 tcccggcaat ctccagtcgt gccggacgaa tatcgcagac tgccgggacc tcggcaccct
      181 gttccaaaag caatcgcaaa aggtatgttc cccgaccacc cacaccaata acgcctattc
      241 gcaagggagc accttgcggc ggggtttctt cagccgaaag gacacctgct gcaactgccc
      301 cggcggcaac tgcggcctga ctcttgagga acatgcggcg gctaagagat gtgtccattt
      361 ggggcaacct ccggaggctt aatcgttggg ccttgctcac ttttgcccct attatgctta
      421 ttcctccggg caaagaaaag cttccaaggc tcgacacaaa gccctggtgg tctggtctgg
      481 actgctgtcc cgactttctc gaaagactac tcctgaggtt cccaataatc gaactgttgc
      541 aattcatccg ggccagggtt ggcgtaatcg gggtctttcg cccgaatgaa cacgtcccca
      601 tcgggaagtg tctttataaa aatgggcaca atcgtcacgc cttggggatt gattgtttgt
      661 gccgtctcgg agagatcctt gccacgggct tcttcgcgcg aaaggggggg cacatttgca
      721 ttataaaagg cggtacacca ccaatggccg gccttgtctt gaaagagggt gccatgtccc
      781 aaaaaccgcc ccacgaagcg gcgaacactg tatggcccct cgatacgatc cgctgtggca
      841 tagtataggt tataacttcc cttacgcatc tgccgggtgg accagcccgt accgaagagc
      901 acatacttgc gtccgaactt cttgatgaag catccttcat gtcccatcgt gcccagagtt
      961 ccgtccggtt cggtggggcc gatcttaata ggctgttggg cgaaatcact aaagtcaggt
     1021 ttaagcggag caatttccgt agccccccag acaagccacc atgtgccatc atcatctcga
     1081 aagagcgacg gatcgtggcg atgaccaatc ttttcgccca tggggttctt ccatggcccc
     1141 ttgatttctg ggccttccgt gagggaaaga ttggaggcac gaacaggcga cggactggtg
     1201 tggaccaacg cccaacgatt gcccagccag tgcaattccg gcgcccacag acgccaagcc
     1261 gattccggca cttgtcggaa gcgttgtggc tgcacctcgg cccaaacccc gtctttcaaa
     1321 ctgaatggct ctccgcagta ctcccagtgg atcaggtccg gactcttcca aagacgcacc
     1381 ttccatccca caatgctttg ggggcccagt cccgtgttca gaatgtcgtt tctttcgcgg
     1441 gcatcgccag gcagggccgt ggtgcccgtc agatagtagt aaccatccgg cgcgagaaca
     1501 ataaaagggt cccgaatcca gccatcctta atgtgaaggg cccgattgtg ggcctgcaga
     1561 ccggcaacga tcgtttccgg tgtgtcggcc gcatggacga atattggcag cagcgtaaag
     1621 ctgattccca gcagaaaaat agcgcgcatc gtaacctccc caagtttaaa cgagattctt
     1681 gcaaacaaca ggctgacgcc acgcccctga aggcctgcgc gtccatcatg atattggcac
     1741 tgtagaagac ctgccgacat gtggcaaggg gcttgcgcac ttgtacattc gatttgcgca
     1801 aaagtccatt gctgacacca gcgcgcttga ccacaatgag gggtgttggt cgtcgtgagc
     1861 acactatttc tatggggagg agacaacgac tatgaatttc tgggcaatgc cttcaatgaa
     1921 agcgaacaaa ctggctcttt cttggttggc atggatgttg acaattgtcg ctttggtcgc
     1981 gacctcttcg gcaacggaaa caagtcagca gcgccgcaac atattgctga tctgcgtcga
     2041 tgacctcaag ccggtccttg ggtgttatgg tgatcccgtg gctaagacgc cgcacatgga
     2101 tcggctggcg gctcgtggca tgcgatttga gctggctttt tgcaatcagg ccgtgtgtgc
     2161 cccatcccgc aataacttgc tgttaggctc gcgttctaca tccattggaa tctatggctt
     2221 gagcgaaaac tttcgccgag cagtacccga tgccgttacg atgcctcagt atttcatgaa
     2281 gtacggctgg cggaccgaag cggttggtaa ggttttccgt accggtcacg ggaaccacga
     2341 cgatgcagca tcttggagcg tgcccagcgt cattgaaaag gtggtggaat atgtggatcc
     2401 tcagaattcg gcaggaggac aactgacgcg agaggaggcc tattttacca atcaaatgct
     2461 ggaccgaatc ggcagtttgc cccgcggtgc tgcctgggag aaacttgatg tcgccgatca
     2521 agcctatgcc gatgggcgaa tcgccgacga gggtatcaag cgattacgag cagccaaaga
     2581 acgccgccag aaggagggta caccattttt cctagccttg ggcttcgtta agccgcatct
     2641 gccttttaca gcaccaaaga aatactggga tctctacagt ccgactgaat tcaagctggc
     2701 ctcttatcaa gagcccccca agggggcgcc gcctgtggca aataaacgcg gtggggaaat
     2761 cttgaactac gatcccctta ctatggaaaa cctgaagacc gatgagattc agcgcacgct
     2821 gatccacggc tattatgcgg ccacaagttt tgcggacgcc caaattggtc gggtgcttga
     2881 tgaacttgac cgactaggac tggcagaatc aacgatcgtt gtcctgtggg gtgataacgg
     2941 tttccatttg ggcgatcacg gtatctggac caaacatacc aattatgagc aggcggtccg
     3001 aattccgttg atcatcgttg ctccgggtgt gacaccgcca ggatcggtaa cacggcaagt
     3061 ggctgaaaac gtcgatatct ttccgacact ggccgagctg gccggactac cgcgccccac
     3121 cggcccccag ccaattgacg gcgtcagcct ggtcccggtt cttcgcaatc ccgatactcg
     3181 cgtggacgat cacgcttacc actgttttcc gcgaggtgcc gtgatgggtc gtgccattcg
     3241 tacagaacgt tatcgactgg tggagtggaa aaagatcggt gccccagcag aaacggcaga
     3301 atatgagctc tacgactacc agacagaccc cgaagaacga gaaaacctca ttg
//
LOCUS       DAOPTR010000369         3351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22674_length_3351_cov_2.859830, whole genome shotgun sequence.
ACCESSION   DAOPTR010000369 DAOPTR010000000
VERSION     DAOPTR010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22674_length_3351_cov_2.859830"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="PKI05_08000"
     CDS             <1..1072
                     /locus_tag="PKI05_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52185.1"
                     /translation="KKLTFRDRGGLDTEKGISVGDEWIYRSYIVGGTEAAGIWTFENL
                     RPEDFPGDTIPVEMNIGVFRTHKGDIERGILGSLLIRNPENGLTVELQVFESKEFSIL
                     ELQIPKTIRKFSSVRFVQRRAKAPDGSIQYSPAQPLNPELLSRTEFDLFKDFVVDGKV
                     EVWLQCLESGQYFGMAKPDLYIRAGNRSFFANLFKGYLGIWLQMVVLVGFGVTLSTFL
                     GTPVTLVSTLGVMIAGMAHQFMVQIVTGELYPEVETPWRGGPLESLIRLVLQEGVMTD
                     LPQTAGTVIARAVDVVFLLWMDLMAQIIPPMERLSLINFVSYGYDVSLSLLAVNAITV
                     VGYLIPLVIVGHFFLRSREVAA"
     gene            1069..3240
                     /locus_tag="PKI05_08005"
     CDS             1069..3240
                     /locus_tag="PKI05_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52186.1"
                     /translation="MTSRQLFIRKIIYLIIMAVLLGVLAYLGTPAAPSRDPQKASPGG
                     VLAQVRELEGLTPTQLGQLDPGGEAIKLATLGMRGVAVHILWMKAEDYKRRKDWSHLS
                     ATLEQMVKLEPHFITVWRHQGWNLSYNVSVEFDDYRERFRWVIRGINFLKEGVRYNER
                     EPILYGDVGWFYSQKIGRADESKQFRRMFMDPTDPFDIHGGRPPRDNWLVGKDWYAEA
                     ERLVDQGAILRTTPLVFHSHRPMCQMNYAEAIEKEGTFGDTAIAAWQTAWHEFVNEFG
                     HRELPSGFDDYPIIRLYEEDILRTQAAELAKKIEELEPGLRNKLLEERRRELTPEERE
                     AYDTPEHDRTVGQAELAFSASIKMKLTHEQVARRISDPKARRQALELARKAMALERQA
                     DIIAHNQQIVNYDYWKRHAEVEQQPEAVAARELMYRSEQAYYNNDHVTAMELFFEAAK
                     KWREVIEKNPDIQYDDHTINDLVEFVDRYMKVLDQLDAIFPEDFALSDIIRRRVRRRP
                     EYQVAQDKLASARKAISKNDFSAARADLEDAIKQYRGMMEFIKCLKLLADRATLNDIM
                     AVIEAYDDLLAKLGDVFPRNFELAELIRLQCLHTPEMRAIKRSVTQAEYLLMQGTGPQ
                     QLAAARQQLEQAVPLAATVLKKFPHLIRLSDRRTAMELAELDLRYRQVLARLGESLPQ
                     DYPLQEFSQKVVPILQKEASEQEGASPSSTSPAASTTDKKT"
BASE COUNT          729 a          917 c          982 g          723 t
ORIGIN      
        1 caaaaaactg acgttccgtg atcgcggtgg ccttgatacg gaaaaaggca tcagcgtggg
       61 cgatgagtgg atttaccgca gctatatcgt aggcggcacc gaagcggcgg gcatctggac
      121 gtttgagaat cttcgtcctg aggacttccc tggtgacacc atccccgtgg aaatgaacat
      181 cggtgttttt cgtacccaca aaggcgacat cgagcgggga atcttgggca gtctgttaat
      241 tcgcaaccca gaaaacggac tgactgtcga actccaggtc tttgagtcga aggagttcag
      301 catcctcgag ttacaaatcc ccaaaacgat ccgcaagttt tccagcgtgc gatttgttca
      361 gcggcgggcg aaagcgcccg atggttccat ccagtatagc ccagcccaac cgctcaatcc
      421 ggaactcctt tcccggacgg agttcgactt gttcaaagac tttgtcgtcg atggcaaggt
      481 ggaggtgtgg ctccagtgcc tagaaagtgg ccagtatttt gggatggcca agcccgactt
      541 gtatattcga gcgggaaacc gttcgttttt tgccaacctc tttaagggct atttgggcat
      601 ctggttgcaa atggtcgtcc tcgtgggctt tggcgtcact ttaagcacct tcttgggaac
      661 accggtcaca ctggtctcca ccttgggggt gatgattgcc gggatggctc atcagttcat
      721 ggtgcagatt gtcaccgggg aactttatcc ggaagtggaa accccttggc ggggaggacc
      781 acttgagtca ttgattcgat tagttcttca agagggggtg atgacggatt tgccccagac
      841 cgccgggact gtcattgctc gggcagtgga cgttgttttc ctcctatgga tggacctgat
      901 ggcccagatc attccgccga tggagcggtt aagcctgatc aactttgttt cttacgggta
      961 tgacgtcagt ttgagtctcc tggcagtcaa tgcgatcacg gtcgtggggt atttgatccc
     1021 cctggtgatt gttggccact tcttcctgcg atctcgtgag gtggcggcat gacgtcgcgt
     1081 cagctattta tccgaaagat catttacttg attatcatgg cggttttgct gggtgttctg
     1141 gcctatctgg gcacacccgc tgcaccgtcg cgcgacccgc aaaaggccag tcccggtggc
     1201 gtgctggccc aagtccgcga gctagagggc ctcacgccca cccaattggg acagcttgat
     1261 cccggtggcg aggccatcaa gttggccacg ctgggcatgc gcggcgttgc cgtgcacatc
     1321 ttatggatga aggccgagga ttacaaacgc cgcaaggact ggtcgcatct ctcggccacg
     1381 ttggagcaga tggtcaaact tgagccccac ttcatcactg tttggcgaca tcagggctgg
     1441 aatctgtctt acaacgtctc ggttgagttc gacgactatc gggaacgctt ccgctgggtc
     1501 atccgcggga tcaatttcct caaggagggt gtccgttata acgaacgcga gcccatcttg
     1561 tatggtgacg ttggctggtt ttatagccag aagattggcc gggcggatga aagcaagcaa
     1621 ttccgccgca tgttcatgga ccccacagac cccttcgata tccatggggg acggccgccg
     1681 cgagacaact ggttagtagg gaaagattgg tatgccgaag ccgagcgcct ggtcgatcaa
     1741 ggggccattt tgcggaccac gccgctggtc ttccattcgc accgccccat gtgccagatg
     1801 aactacgcgg aagccattga gaaagagggg acctttggcg ataccgccat cgcggcatgg
     1861 caaacggcgt ggcatgaatt cgtcaacgag ttcggccacc gagagctgcc gagcggcttc
     1921 gatgattatc ccatcattcg gctgtacgaa gaggacattc tcaggactca agcggctgag
     1981 ctggccaaga agatcgagga attggagcct ggcctccgca acaagctgtt ggaggagcga
     2041 cgtcgtgaat tgactcccga ggagcgcgag gcgtacgaca ccccggaaca cgatcgaacg
     2101 gtagggcagg cggagttggc gttctcggct agcatcaaaa tgaagctgac acatgaacag
     2161 gtggcgcggc ggatttccga ccccaaagcg cggcggcagg ccctcgagct tgcccgcaag
     2221 gcgatggcct tagaacggca ggccgacatc attgctcata accaacagat cgtcaattac
     2281 gattactgga aacgccatgc cgaggttgaa caacagcccg aggccgtggc cgcacgggaa
     2341 ctcatgtatc gttctgagca ggcctattac aacaacgacc acgtgacggc catggagctg
     2401 ttctttgagg cagccaagaa gtggcgggaa gtcatcgaaa agaatcctga catccaatat
     2461 gacgatcaca cgattaacga tctggtggaa ttcgtggatc gctacatgaa ggtcctcgac
     2521 cagttggacg ccatcttccc cgaagatttt gccctttcgg acatcatccg gcggcgcgtg
     2581 cgtcggcgtc ctgagtatca ggtggcccag gataagctcg cctcggcccg gaaggccatc
     2641 agcaagaatg acttctcggc ggcccgcgcc gacctcgaag atgccatcaa gcaataccgc
     2701 ggcatgatgg agttcatcaa gtgcctgaag ctccttgccg atcgagccac cttgaatgac
     2761 atcatggccg tgattgaagc ctacgatgat cttctggcca aattgggaga cgtgttcccg
     2821 cggaatttcg aactggcaga gctgatccgg ttgcagtgtc tgcacacgcc agaaatgcgg
     2881 gcgatcaagc ggtccgtcac gcaggcggag tacctcctga tgcaggggac aggtccgcag
     2941 caattggcag cggcacgtca gcagctcgaa caagccgtgc ctttggcggc taccgtgctt
     3001 aaaaaattcc cgcacctcat acgcttatcc gatcgccgga cggcgatgga actggcagag
     3061 cttgatctgc gataccgtca agtcctggca agattgggcg aatctctgcc ccaggattac
     3121 ccactccagg aattcagcca aaaggtcgtg ccgatcctcc agaaggaagc ctccgagcag
     3181 gaaggtgcat cgccatcgtc cacgtccccg gccgcttcca ccaccgacaa gaagacatga
     3241 cacaccccgt ggaggttgat ctgccctcca aggcgtgaca cggctttcac tgcgtgggct
     3301 ggttcattcg tacagccgcc ggtgcaagct ggcggtgtgc tgtgttcgct g
//
LOCUS       DAOPTR010000370         3349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22689_length_3349_cov_3.149970, whole genome shotgun sequence.
ACCESSION   DAOPTR010000370 DAOPTR010000000
VERSION     DAOPTR010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22689_length_3349_cov_3.149970"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="PKI05_08010"
     CDS             complement(<1..681)
                     /locus_tag="PKI05_08010"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA52187.1"
                     /translation="MTRKSQKEYRLSRRDLLAKSAKSVVAVALASQFVGCPKQEGAPQ
                     IIPESAKGANERIGVGIIGCGRRNGQLVTGKGGQGKPPKEAQIVAVADVNLRRAQQWA
                     EKYKCPAFQDYRKLLEMKEVDVVIYATPEHSHYLPCIHACQAGKDIYGEQPLSHTIRE
                     GQVMIQAVRKYKRVFQVGEQQRSHPATRKAVELILNGRIGKVHTVIGYNYPSPWDAEF
                     PAEQVPPDL"
     gene            complement(790..2154)
                     /locus_tag="PKI05_08015"
     CDS             complement(790..2154)
                     /locus_tag="PKI05_08015"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOA52188.1"
                     /translation="MSHFTTDRRAFLAKLGVLGASAWASGLAFGNVDVPKDEEKLAID
                     GGKPVREKMLAARPYGPQFYDDREKTELIEVLESRQPFRWWHGNSKVLQFEQAYAAHL
                     GVKYALGVTSGTTALVTAMAALEVGPGDEVILPAWTWYADYDAIVLAGALPVFAEVDE
                     SFNIDPNDIEAKITPQTKAVIVCHLQGTPADLEPIMAIAKKHGVRVLEDFAQCVGGKY
                     KGRYVGTMGDIGINSFQLSKTITSGEGGAVVTNDATLFERALRFHDVGSLRSPYTDLL
                     KGGLLAAFAGCNFRMSEFTGAVLKAQLEKLETICAKVRANARFVREGLSDLPGIKFRK
                     SPDIDGDIGVGVYLDLGNRARRDRFLRALRAEGVSAVGPVGSVILPTDKRIENKVTVH
                     PEWPSFRSQRGREIQYGAACCPRTIDIINRFGGVIIDPNFTEDELKDIVHAMRKVYLA
                     TRDV"
     gene            complement(2251..>3349)
                     /locus_tag="PKI05_08020"
     CDS             complement(2251..>3349)
                     /locus_tag="PKI05_08020"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOA52189.1"
                     /translation="YWNGPQTALLKKRFQEVCPLKYVQTCSSGTAAIHIAVASAGIGL
                     GDEVITSPITDIGTVIGIIYQQAVPVFADLGPGTYNLDPTDVERRITPRTKAIIAVHL
                     TGNPCDMDRLKDIAKRHNLVLIEDCCQAWGAKYRGRPIGTIGDFACFSLQDSKHVTCG
                     DGGIVATSDERFGPNIQRFGDKGYDRTRGLGLEVFATNYRMSEPQAAVAAAQLTRLEE
                     VASRRYQLGTLLGELLADVPGIIPHQVHPDDRPVYWFYMFRIKPEALRCNRSQFTRAL
                     QAEGVQASGGYIAKPLYKEPVFQNHAFFAGRWPVKEMGLTTMDFTKHRCPEAEAILET
                     CIRVPIKEFMDEEYIRQVGAAIRKVARYYSA"
BASE COUNT          756 a         1017 c          806 g          770 t
ORIGIN      
        1 aagatctggt ggcacctgtt cagccggaaa ttctgcgtcc catgggcttg gataattata
       61 accaatcacc gtgtgcacct tgccgatgcg accgttcaaa atcagttcga cggcctttcg
      121 cgtagcggga tgcgaacgtt gctgctcacc gacctgaaag actcgcttat actttcgcac
      181 cgcttggatc atcacctgac cttctcggat ggtgtgactg agcggttgct cgccgtagat
      241 atccttgccg gcctgacacg catggataca cggcaaatag tgggaatgct ccggcgtcgc
      301 gtaaatgaca acatcgactt ccttcatctc caggagtttt ctgtagtcct gaaacgcggg
      361 acatttgtat ttttcagccc attgttgtgc ccgtcttaaa ttgacatctg ccaccgcgac
      421 aatttgagct tccttggggg gtttcccctg tcccccctta ccggtcacca attgtccatt
      481 tcttcgtcca cacccgataa tgcccacgcc aatccgttca tttgctccct tggcggattc
      541 cgggatgatc tgcggtgcac cctcttgctt gggacacccc acaaattgcg aggctaacgc
      601 tacggcgacg accgactttg ccgactttgc caacaaatcc cggcgcgata aacgatattc
      661 tttctgagat ttcctagtca tggcagagct ccactatcat gccagcggat acaagcacaa
      721 ccctcgtttc ccccaacttt atctgctaaa acactgcacg agaaataatc aggatcacct
      781 ctgatacctt cagacatcgc gcgtcgccag atacaccttg cgcatcgcgt gaacaatgtc
      841 ttttagctca tcctcggtga aattcgggtc tatgatgacg ccaccaaagc gattgatgat
      901 gtcaatcgtt ctcgggcaac atgcggcccc gtattggatt tcgcgtcctc gctggcttcg
      961 gaaggagggc cactcgggat ggacggtaac cttgttttca attcgcttgt ccgtcggcag
     1021 gatgaccgat cccaccggtc ccaccgctga aactccttcc gcacgcaagg cacgcaaaaa
     1081 ccgatcgcgt cgcgcccgat tacccaagtc gaggtacact ccaacaccga tatcgccatc
     1141 gatatccggg gacttgcgga acttaattcc cggcagatcg ctcaggcctt cgcggacaaa
     1201 acgtgcattt gcccgaacct tcgcgcaaat agtctcaagc ttttccaact gggccttaag
     1261 caccgccccg gtaaactcgc tcatacggaa attgcaacca gcaaaggctg ccaatagtcc
     1321 ccccttaagc agatcggtgt atggtgaacg caacgaacct acatcgtgga accgcaaagc
     1381 cctttcaaac agcgtcgcat cgtttgtcac gacggcaccg ccctcgccag aggtaatcgt
     1441 cttgctaagt tgaaaactgt tgatgccgat atcgcccatc gtgcccacat atcgcccctt
     1501 gtattttcca ccgacacact gggcgaagtc ttcgagaaca cgaaccccgt gcttcttggc
     1561 aatcgccatg atcggctcaa gatcggccgg tgtcccttgg aggtggcaga caatgacggc
     1621 ctttgtctgc ggagtgatct tagcctcgat gtcgtttggg tcaatattga acgactcatc
     1681 cacttccgca aataccggaa gtgccccagc caggacgatc gcatcgtaat cggcatacca
     1741 ggtccatgcg ggcaaaatga cctcgtcacc cggtcccact tcgagcgcgg ccatggcggt
     1801 gacaagcgcc gttgtcccgg aggtcactcc caaagcatac ttgacaccca agtgagcggc
     1861 ataggcctgt tcaaactgca gaacctttga attcccatgc caccaccgga acggctgtcg
     1921 cgattcaaga acttctatga gttccgtctt ttctcgatcg tcatagaatt gcggaccgta
     1981 tggtcgtgcg gccaacatct tttcccgcac gggttttccg ccgtcgatgg ccagcttttc
     2041 ctcatccttg ggtacatcca cattcccgaa tgccaacccg ctggcccacg cagacgcccc
     2101 caaaactccg agtttcgcca aaaaagcgcg acggtccgtt gtaaagtgag acatcgccgt
     2161 accctcccaa aaaatgcacc tgaacccttg taggatttgc gggactctcc caaacacggg
     2221 ttcagagccg aggactacga aacgtttcta ttatgccgag taatacctcg caacctttcg
     2281 aatggctgct cccacctgcc gaatgtactc ttcatccatg aactccttga tcggtacccg
     2341 aatgcacgtc tcaaggatgg cctccgcttc gggacaccga tgttttgtga aatccatcgt
     2401 cgtcagaccc atttccttaa caggccagcg tccggcgaag aacgcatggt tttggaatac
     2461 gggttctttg taaagcggct tcgcaatata tccgcccgat gcctgcactc cttctgcctg
     2521 caacgcccgc gtaaactgag accgattgca acgaagggct tccggtttga tgcggaacat
     2581 gtaaaaccaa tacacgggtc gatcatccgg atggacctgg tggggaatga ttcctggcac
     2641 atcagccagc aactcgccca aaagggtccc cagttgatag cgccgcgaag cgacctcttc
     2701 caagcgcgtt agttgagcgg ctgccacagc ggcctggggc tcactcatgc gataattggt
     2761 cgcaaacact tctaacccga gtccccgcgt ccgatcatat cccttgtcac caaaccgttg
     2821 gatattgggc ccaaatcgtt cgtcacttgt ggcgacaatc cccccatcgc cgcaggtcac
     2881 gtgtttactg tcctgtagcg agaagcacgc gaaatcaccg atggttccga tcggccgacc
     2941 acggtatttg gccccccaag cttggcaaca gtcctcgatc agaaccagat tatgtcgctt
     3001 ggcaatgtct ttaagccgat ccatatcaca agggttaccg gtcaagtgaa ccgcgatgat
     3061 cgccttggta cgaggcgtaa ttcgccgctc cacatccgtt ggatcgagat tatatgtccc
     3121 cgggccaagg tcggcgaaaa ccggcacagc ttgctgatag atgatcccga tcacagttcc
     3181 gatgtcagtg atgggagatg tgatcacttc atcaccgagg ccaattccag ccgatgccac
     3241 ggcaatgtgg atggctgccg tgcccgaaga acaggtctgg acatacttca gcggacaaac
     3301 ctcttggaac cgcttcttga gaagggccgt ttgggggcca ttccagtag
//
LOCUS       DAOPTR010000371         3349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22692_length_3349_cov_3.074985, whole genome shotgun sequence.
ACCESSION   DAOPTR010000371 DAOPTR010000000
VERSION     DAOPTR010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22692_length_3349_cov_3.074985"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PKI05_08025"
     CDS             <1..440
                     /locus_tag="PKI05_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOA52190.1"
                     /translation="ITGLLGVMERRFQERAFQVAWEAANQRNPRPALLDNGNPTTGGG
                     YTVMVDCTADWCLTCKTLEATVLNTERVKAVIRKNNVILLKADWTHAAPEVTQFLDWL
                     GFRQVPVLAIFPAEKPNQPIVFTGGYTQKDVIDALNRAGPSRN"
     gene            complement(458..1192)
                     /locus_tag="PKI05_08030"
     CDS             complement(458..1192)
                     /locus_tag="PKI05_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52191.1"
                     /translation="MVPTLFLIALAGTAVDVGWKPTPDGRIEYIIQVTPEEVQNLNIG
                     DIIAASDVPPGLPPIAAYRIVVGRGPLPRELPDHPSTTEPRETIASDPRQDNDPSKVT
                     RVAQPAVGVFPADAEQLPAIPTEAGPTSGSSQPSDPAKARNASSEDAVPSNAAENAPD
                     RPQPENLSNDSSSQSHVLPVVAAILFALCLASVALAAWIATDYRRKYRELVKNVDSSL
                     LPSNDSQESPAPSEDTNTPKVQQSPS"
     gene            1423..2361
                     /locus_tag="PKI05_08035"
     CDS             1423..2361
                     /locus_tag="PKI05_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52192.1"
                     /translation="MYFSRAYLVSVGVFWLGAMGWLVMEKIVPTLLVGNPPRYSDIVD
                     SYRQQKAIGWTIQWRDREIGWALIRTEVLGDDLTEVKNVLQFRELPIHEIFPLIGQVL
                     AAELPVSRLRLRVETNLLFDPLKRLSSFQTRLKTPNSQEPLVVVRGSIDKGKLQLSIQ
                     SGVFVYDTELPFQSNTLLTDSFSPQATLPNLREGQKWTIEVYSPIQLPQQAVGARPLE
                     VVHAEVQGQEMILWQQELHKCWVVVYKSDPAESLNSRREPRGRMWVDSKGRVLKQEAR
                     VGGGKIVLVRLGEKETARLVRGLDAESWPTANKGIK"
     gene            2358..3248
                     /locus_tag="PKI05_08040"
     CDS             2358..3248
                     /locus_tag="PKI05_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA52193.1"
                     /translation="MIVFSDVTKRFGPKTAVEHLNLSIPRGELFAFLGPNGAGKTTTI
                     RMLVGLLRPNEGTVAICGFNTATQPREAARLIGYVPDQPYLYDKLSGREFLEFVGQLR
                     GLPLPVLKDRIDREIERFGMSEFVDDLTETYSHGMRQRLVFASAVLHDPSVLIVDEPM
                     VGLDPKSAKLVKDLLKSKAAEGASVFMSTHTLSIAEEIADRIGIIHKGRLIFAGSVEE
                     LRALRASDHASLEEIFLELTDEAATELSQSDPAWASKDPPSHSDPVEQPHSDWKNSEI
                     LAKTETKEVLGRAVEERSNT"
BASE COUNT          755 a          801 c         1002 g          791 t
ORIGIN      
        1 gaatcacggg cctgcttggt gtgatggagc ggcggtttca ggaacgtgcc ttccaggtgg
       61 cttgggaagc tgcgaaccag aggaatcctc ggccagcttt gcttgacaat ggaaatccga
      121 ccaccggcgg cggttacaca gtgatggtgg attgcacagc cgattggtgt cttacctgta
      181 aaaccttgga ggccacggtc cttaacactg aaagagtaaa ggccgtgatt cgcaaaaata
      241 atgttatcct tttgaaagcc gattggaccc atgccgcccc cgaggtgacc caatttctgg
      301 attggctggg cttccgacaa gttcctgtct tggccatatt tcccgcagaa aagcccaacc
      361 aacctatcgt ctttaccggc ggctacaccc agaaggacgt gattgacgca ctcaataggg
      421 ctggtccatc acggaactaa ctcaccgtgt gttgatttca tgagggcgat tgctgcactt
      481 tgggcgtgtt ggtgtcctcg gatggggcag gggattcctg ggaatcgttt gagggcagga
      541 gacttgaatc gacgttcttg acaagttctc gatacttcct gcgatagtcg gtagcgatcc
      601 atgccgcaag ggcaacagaa gccaaacaga gggcgaagag gatcgcggcc actaccggga
      661 ggacgtgcga ctgggatgaa gagtcgttgc ttagattctc gggttgtggc cgatcaggtg
      721 cattctcggc ggcatttgag ggtaccgcgt cttctgatga cgcattgcgt gctttggccg
      781 ggtcactcgg ttgtgagctg ccgctggtcg gaccagcctc ggtgggaatg gctgggagct
      841 gttccgcgtc ggctgggaag acacctacgg ccggttgcgc cacacgggtg actttgcttg
      901 gatcattgtc ttgtcgcgga tcactggcga tagtttcacg aggttctgtt gtggaggggt
      961 gatcgggcaa ttcccgcggc aaaggacctc ggccgacaac gatccgatag gcggcgatcg
     1021 gaggcaatcc agggggcaca tcgctggcgg cgatgatgtc tcctatattc agattttgca
     1081 cttcttcggg ggtcacttga ataatgtatt caatccgccc gtcgggcgtc ggcttccacc
     1141 cgacgtcaac cgcggtccct gccaaagcga tgaggaatag cgtgggaacc atacgtgcag
     1201 tgatcggttt atcctaaaag ccaggcctgc cctttggcat ctcaggcgtc tgtaacaacg
     1261 gggcaaatct cgcccgggca acgacaacca ctttggattg tgattcattg tcacgtttga
     1321 tgcaaccgca cgaccttcgg gtaattgttt cctcatctta caagtttggt aaaatgatca
     1381 agtggggtca tgggtttcgg atgggagaag gtgccttttc atatgtattt cagccgtgcg
     1441 tatttagtaa gtgttggggt cttctggttg ggagccatgg gttggctggt catggagaaa
     1501 attgtgccaa ccctgttagt gggaaaccct ccccgttact ctgacattgt cgatagttac
     1561 cgccaacaaa aggcgatcgg atggaccatc caatggaggg atagggagat cggctgggcg
     1621 ctgatacgga cggaggttct cggggatgac ctcacggagg taaaaaacgt cttgcagttc
     1681 cgcgagttac cgatccacga gatatttcct ttgattggcc aggtcctggc cgctgaattg
     1741 ccggtgagcc ggttgcgctt gcgcgtggaa actaacctct tgttcgatcc cttgaaaagg
     1801 ctttcatcat tccaaacgcg tctcaaaacg cctaattccc aggagcccct tgttgtcgtg
     1861 cggggcagca ttgacaaagg aaaattgcag ctttccattc aatcgggcgt atttgtgtat
     1921 gatacggaac tgcctttcca atcgaacacg ctgctaacgg attcgttttc gcctcaagca
     1981 actttgccta atttgagaga gggtcagaag tggaccatag aggtgtacag cccgatccag
     2041 cttccccaac aagccgtggg ggcgagaccc ttggaagttg tccacgcaga ggtacagggt
     2101 caggagatga ttctctggca gcaagaactg cacaaatgct gggtcgttgt gtataagagc
     2161 gaccctgcag agtcgctaaa ctcccgccgt gagccgcggg gtcgcatgtg ggtggactca
     2221 aaagggcggg tattgaaaca agaggctagg gtaggcgggg ggaagattgt gcttgttcgt
     2281 ttgggagaaa aagaaacggc aagattggtt cgtgggctgg atgcagagtc gtggccgacc
     2341 gcaaataagg gaatcaaatg atcgtctttt ctgacgttac aaaacggttt ggaccaaaaa
     2401 cggcagtgga acatttgaac cttagcattc cgcgggggga actgtttgcg tttttgggac
     2461 ctaatggggc aggtaagacg acgaccattc gtatgctcgt cggtttgctt cgtcccaatg
     2521 aaggcaccgt ggcgatttgt ggttttaata cggcaacgca gcctcgtgag gccgcccggc
     2581 tgattggcta cgttcctgac caaccgtatc tttacgacaa gttatccgga cgcgagtttc
     2641 ttgagttcgt gggtcagctt cggggacttc ccctgccggt actcaaggat cgtattgacc
     2701 gggagattga gcgttttggt atgagcgagt ttgtggatga tctgaccgag acgtactccc
     2761 acgggatgcg gcagcggctt gtctttgctt cggccgtctt gcatgatccg agcgtcctca
     2821 tcgttgatga accgatggtt ggcctcgatc cgaagagtgc caagctggtg aaggaccttt
     2881 taaagagcaa ggccgcggaa ggcgcatcgg tgtttatgtc cacccatacc ttgagtattg
     2941 cggaagaaat tgctgaccgg atcgggatca ttcacaaggg aagactcatt tttgccggct
     3001 cagtagagga attacgggct ctgcgagcaa gtgaccacgc ctcgttagaa gagatctttt
     3061 tggaacttac agacgaagcc gcgacagaat tgtcacaaag tgacccagcg tgggcgtcca
     3121 aggacccacc cagtcattcc gacccggtgg agcaaccaca ttcagattgg aaaaacagcg
     3181 aaatccttgc gaagaccgaa acaaaggaag tgcttgggcg cgccgttgag gaacgctcaa
     3241 acacgtgaaa tccccaacaa gtgaggaaac tggcaaagac tctgccgggg tccgtttgga
     3301 aatcaatcga tgactatgac gacgggtaac gtggaaggca tttgtattt
//
LOCUS       DAOPTR010000372         3334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22810_length_3334_cov_3.550473, whole genome shotgun sequence.
ACCESSION   DAOPTR010000372 DAOPTR010000000
VERSION     DAOPTR010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22810_length_3334_cov_3.550473"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="PKI05_08045"
     CDS             <1..726
                     /locus_tag="PKI05_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52194.1"
                     /translation="KNRGLIYAMFETSVFHDDLYAMEKLYAANVFGHIVYAEGEYCHP
                     HPIGAPSLPSFRDWRKNGCPMWYPTHATAYYVGVTHKPLVSVSCQGTPDFDPSRRIPN
                     AIGNIFRSEVGLFRTEEGGLCRIIICRSQGEYLEAGRIRGEYGGFNQKFSGDETGKKR
                     YEEALAQGLSLKKPGLPPNVRPGGHGGSHGYLAHDFIDAILRNRKPAVDIIDALHMTV
                     PGYYAHLSALKDGETMRIPTYSL"
     gene            complement(1173..3137)
                     /locus_tag="PKI05_08050"
     CDS             complement(1173..3137)
                     /locus_tag="PKI05_08050"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOA52195.1"
                     /translation="MHVFKSLLTSMVVVSVISSANGEGLSQPGNPSPSPNAVRAGRFH
                     VEPPTLLNLGFEWAIEGDANRNATVDVRFRQVGEIEWRQALPLVRIGGERVFREREGL
                     DYTVPDGFAGSILNLQPGTEYECQFLMRDPDGVEGQAVHTVRVKTRSEPQPFPGGRVL
                     HVYPPDYQGERQEPSFSSIMQAYYGAGLGDWNVVWEHRVQPGDTILVHAGLYKPDRRN
                     YVDPLCTPFDGTTYLTLKATPDRPITIKAAGDGEVIFDGGGNHRLFDVMASAYHIFEG
                     LTFRNTDVAIFAGQKGVTGAVGLTVKNCRFENVGFGVWTESPYSREFYIADNLFIGRE
                     DRFRLIGWTGPLWPSAGRYGSHELVSYYAIKVYGPGHVITHNAIAYFHDGIGISTYGT
                     PENDPDLRASSIDIYNNDIHMTNDDFIETDGAVHNVRVFRNRGVNAAQGGYSAQPSFG
                     GPVYFYRNILYHVPTGVSFKFSANPAGLFVFHNTIIGEQVGMGPSSNVHWRNNLFVGT
                     DAPGRAIMSWNNATESYSSDYNGYRPNRGVSAQYRWRVPTAAQPQVFATLAEFRSATA
                     QEKHGIEVDYDVFENLRPPDPSNRHAVYHAMDLNFRLNPHGAAVDAGVIIPTVNEDFT
                     GNGPDLGALELGQSEPHYGPRWLTWQPFYR"
BASE COUNT          756 a          913 c          894 g          771 t
ORIGIN      
        1 aaaaaccgtg ggctcatcta cgcgatgttc gagacctccg tctttcacga tgacctgtat
       61 gcgatggaga agctctacgc ggccaatgtc tttgggcaca tagtttacgc ggaaggagaa
      121 tactgccacc ctcacccgat cggtgctcct tcactgccca gttttcgcga ctggcgaaag
      181 aacggctgtc ccatgtggta ccccacgcat gctacggcgt attatgtggg agtgacccat
      241 aaacctttgg taagtgtttc ctgccagggg acgcccgact ttgacccatc gcggcgcatc
      301 cccaacgcca ttggcaacat ctttcggtcg gaggtcggtt tatttcggac ggaagaaggt
      361 ggcctgtgtc ggatcatcat ctgccgatcg caaggagaat atcttgaggc tggtcggatt
      421 cgcggtgaat atggcgggtt caaccagaag ttcagcggtg atgagacagg gaaaaagcgc
      481 tacgaagagg ccctcgctca gggtttatca ttgaagaaac cgggattacc gcccaacgtt
      541 cggccaggcg gccacggagg ttcgcacggt tacttagccc atgatttcat tgacgccatc
      601 ttgcgtaatc ggaaacctgc cgtggatatt atcgacgccc tgcatatgac cgtccccgga
      661 tactatgcgc atctttcagc cctcaaagac ggggaaacca tgcgcattcc gacatattcg
      721 ctctagtatc ggtcgtggtg cgacgttgta aatgtaaaag cagtgacaaa ccggcacggt
      781 aagtttttcc aattcactcc acaaccgaca aggccttcgg cggttggtgg gtgcagtaga
      841 ccggcctctt ttggaaacac caacttgaac ctgtcgccgc acgttttagg tcactgagcc
      901 ttgcagaggc gtccaatagg tccgtcatcc caacgatgct atcaagacat gggctgagcc
      961 ctgattgtac cccaccatcg gctgtcacac gtccacggca tcaacggcct cgcggcgaag
     1021 cgttcgccgt tggatatcgc gcttacaagc attgatgggc aagtcgattg tttaagccgc
     1081 gatccgctct tctatggtat ccgatacgtc tttttaaagg catattccac aaatttatca
     1141 gattcgagcg gaatcccagg ggcaaccctg cgttaccggt agaacggttg ccaggtaagc
     1201 catcggggtc cgtagtgggg ttcgctttgt cccaattcta aggcccccag atctgggccg
     1261 tttccggtaa agtcctcgtt gacggtggga atgattacgc cggcatcgac tgcggcaccg
     1321 tgcggattaa ggcgaaagtt gagatccatc gcgtggtaca ccgcatgacg gtttgaagga
     1381 tcaggtggcc tgagattttc gaagacatca taatccactt caatcccgtg cttctcctgg
     1441 gctgttgccg agcggaactc agccagggtg gcaaaaacct ggggttgagc cgctgtcggc
     1501 acacgccacc tatactgggc ggaaacgccg cggtttggtc ggtagccgtt gtagtcggag
     1561 ctataacttt ccgttgcgtt gttccacgac ataatggcgc gacctggtgc atccgtgcca
     1621 acaaacagat tgttgcgcca atggacattt gacgagggcc ccatgcccac ctgctcacca
     1681 ataatcgtgt tatgaaacac aaatagaccg gcagggtttg ccgagaactt aaaggaaacc
     1741 cctgtcggca cgtggtacaa gatatttcgg tagaaataca ccggaccgcc aaacgacggc
     1801 tgcgcgctgt aacctccttg agccgcattg acgccacggt ttctaaagac gcgcacgttg
     1861 tgtacagccc catcggtttc gataaaatcg tcattcgtca tgtggatgtc gttgttgtaa
     1921 atgtcgatag aggatgccct gaggtctgga tcgttttccg gcgtgccata tgttgaaatg
     1981 ccgatgccat catggaaata agcgatggcg ttgtgtgtaa tcacgtgtcc cgggccatac
     2041 accttaattg cgtaatagct caccaactca tgcgaaccat agcgtcctgc acttggccac
     2101 aacggtcccg tccagccaat gagccgaaag cgatcttcgc gaccgatgaa gaggttgtcg
     2161 gcgatgtaga attcgcgtga ataagggctt tcggtccaaa caccaaagcc cacattctca
     2221 aatcggcagt ttttcaccgt cagacctacg gcccctgtta cgcctttttg tcccgcaaaa
     2281 atggctacat cggtattccg gaacgtgagc ccttcgaaga tgtgatacgc ggaggccatc
     2341 acgtcgaaca agcgatggtt accgcctcca tcgaagatga cctccccgtc accggcagcc
     2401 ttaatggtaa tgggtcggtc tggcgttgct ttgagcgtaa gataggttgt gccgtcgaag
     2461 ggggtgcaca gcgggtccac gtagtttcgc cgatcgggct tgtatagccc cgcatgaaca
     2521 agaatcgtgt cgcctggctg gacacggtgt tcccacacca cattccaatc acctaatccg
     2581 gcgccgtagt aggcttgcat aatgctggaa aaactgggtt cctgtcgttc cccttggtaa
     2641 tccggcggat aaacatggag cactcgtcca ccgggaaacg gctgcggttc ggatcgggtc
     2701 ttgacacgaa cggtgtgaac ggcttgtccc tccacaccgt cggggtctcg cataaggaac
     2761 tggcactcgt attcggtgcc tggttgcaaa ttcaagatgc tgcctgcaaa gccatcgggt
     2821 acagtgtagt ccaggccttc tcgctcgcgg aacaccctct caccaccgat gcggacaagg
     2881 ggcagcgctt gtcgccattc gatttccccg acttgtcgga agcgaacatc cacggtcgcg
     2941 tttcgattgg catctccttc aattgcccat tcaaagccga gattaagcag agtgggcggc
     3001 tccacgtgaa aacgacccgc ccgcacggca ttgggcgaag gactagggtt gccaggctgc
     3061 gagaggcctt caccgttggc gctgctaata accgagacca caaccataga cgttaggagt
     3121 gacttgaaca cgtgcattac tagaactccc ttagcgaccc gtttatacga tctgaactgc
     3181 ttcccccata gtttgttttc tttcctcatg agagtcaaat aaaattggtg atcgcatcta
     3241 aacatagtcc acccggtaga gggacttggc ccgttgaggt aagtcacgcg attgtaagac
     3301 cacttgttga tgaagtcccc gccaactaac cgtt
//
LOCUS       DAOPTR010000373         3329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22850_length_3329_cov_3.498167, whole genome shotgun sequence.
ACCESSION   DAOPTR010000373 DAOPTR010000000
VERSION     DAOPTR010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22850_length_3329_cov_3.498167"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..442
                     /locus_tag="PKI05_08055"
     CDS             56..442
                     /locus_tag="PKI05_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOA52196.1"
                     /translation="MKIVIAIVRPFVAEKILEALKFAPVEACTVREVKGFGRQKNYLE
                     EYRGGDYALAFIPKVQITIWVDDLRTEEIIRLIVSNARTGRMGDGKVFVLSADFTGTE
                     SFDLARAGEHEENEELPEDGFTGFFG"
     gene            557..1180
                     /locus_tag="PKI05_08060"
     CDS             557..1180
                     /locus_tag="PKI05_08060"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOA52197.1"
                     /translation="MSGKSSEIRNYFSRLIDELNRLNLRELEDFSQVLFDAWLHRRLV
                     FVCGNGGSAATASHLAQDLAKGTIRDADLLSYAVPRFKAISLVANISWLTALGNDLGY
                     EQVFVQQLANYAQPGDVLIAISGSGNSPNILAAVEWANDHELMTLGMTGFDGGRLRAM
                     QKKGIHVPLNDMEMVESIHSCICHWLVNDLRQRIYEAIVSSATRESK"
     gene            complement(1246..2079)
                     /locus_tag="PKI05_08065"
     CDS             complement(1246..2079)
                     /locus_tag="PKI05_08065"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HOA52198.1"
                     /translation="MSLDTTAMNHPKEVHKRDPLFRFQMFTSLSLLVLFLIVTLSGAI
                     LYSSPRGRVANWGQWTVLGWDKESWAAFHINATICFVVFSILHLLFNWKMLLGYLKRG
                     TLGLVRGRLELLAALVFAGLLYAGTRYHVPPFSQFVAWREAFKDYWERRSPPAPIPHL
                     EEFTLERISQLAGASEEEILQFVRSLGVEDVKSSSTLAQIAAQIGQSPRQVFDAMAQR
                     FPSLQAIQPPSPEQRRGLRRPDGSALEGPGAFGPGSGRGGGRGMGQGLRRGRMSDPNF
                     E"
     gene            2458..>3329
                     /locus_tag="PKI05_08070"
     CDS             2458..>3329
                     /locus_tag="PKI05_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA52199.1"
                     /translation="MVRLKWLRFVLAAVGLASINMGDAAAQEDSPPNIVFIYADDLGY
                     GDIGCYGATRIPTPNLDRLAKEGLLFRDAHSPAATCTPSRFALMTGQYAFRKPGTGVL
                     PGNADLIIEPGQLTLPLMLKRAGYITSVVGKWHLGLGRNGCDWNGTIAPGPLEIGFDY
                     AFIIPATGDRVPCVYVENHHVVGLDPNDPIQVSYGEKIGDEPTGREHPELLKYRFSHG
                     HDGTIINGISRIGFMKGGKSARWVDEDMADVITQKAVSFIESHKDQRFFLYFATHDIH
                     VPRLPHPRFVGKSQ"
BASE COUNT          805 a          846 c          900 g          778 t
ORIGIN      
        1 gtctggccgg gtaggaccgc gtcgctctgg ttaggaacca tagcgaaaga agcgcatgaa
       61 aatcgtcatc gcaattgttc gaccgttcgt tgccgagaag attctcgaag cgctgaagtt
      121 cgcgccggtc gaggcgtgca cggtccggga agtcaagggt tttggtcgtc agaaaaacta
      181 tttggaggaa tatcgcggcg gcgattatgc cctggccttc attcccaagg tgcaaatcac
      241 aatttgggtc gacgacctgc ggaccgaaga gatcattcgc ctcattgtca gcaatgcccg
      301 aacaggacga atgggtgatg gaaaggtctt tgtcctttcg gcagatttta cgggtaccga
      361 atcgtttgac ttagcgcgtg ctggggaaca tgaagaaaac gaagaactgc ccgaagatgg
      421 gtttactggc tttttcgggt aagatttttc aacgatcatt tgtagaatac gcatctcgcc
      481 ggcgggtata taccatcaat gagaccggag attccgcgaa agggccggta gatgatgctt
      541 agaaggagtg cgatacgtgt ccggcaaaag ctcagaaatt cgcaactatt tttcgcgact
      601 tattgacgaa ctgaaccgcc tcaatctgcg agaactggaa gacttttctc aggtgctgtt
      661 cgatgcctgg ctgcaccggc ggttggtgtt cgtctgcggg aatggaggtt ccgcggccac
      721 ggcaagtcac ctggcacaag accttgccaa aggcacgatc cgcgatgccg atctactctc
      781 gtatgctgtg ccacggttta aggccataag tctcgttgcg aacatcagtt ggctcactgc
      841 gttgggcaac gatctaggct atgagcaagt ttttgttcag caactggcga actacgctca
      901 gcccggtgat gttcttattg cgatcagtgg ctcaggcaat agccccaaca tcttggcggc
      961 agtcgagtgg gcaaacgacc atgagcttat gacgcttggc atgacggggt tcgatggagg
     1021 acgattgcgt gccatgcaga aaaaggggat tcacgtcccg ctaaacgata tggaaatggt
     1081 tgagagtatc cactcgtgca tttgccattg gttggtcaac gacctgcgtc agcgcattta
     1141 cgaggccata gtctcttcag ccacgcggga atccaagtaa tcctttcaca aagtggtacc
     1201 actgcgccgg gcaatggtca caagaaggac ggctgttccc ggaagtcact caaagttggg
     1261 atcactcatt cgaccacggc ggaggccctg ccccattccg cgtccacccc cacggccaga
     1321 ccctgggcca aaggctccgg gcccctccag agcactgcca tcaggcctac ggagtcctcg
     1381 tctctgttcc ggcgagggag gttgaatggc ctggagtgag ggaaatcgct gcgccatagc
     1441 atcgaatacc tgacgaggag actgtccgat ttgtgcagcg atctgggcaa gcgtgcttga
     1501 acttttcaca tcctcgacac caagcgatcg tacgaactgg aggatttcct cctcagacgc
     1561 cccggctaat tgggagattc gctcaagtgt gaactcttcc aaatgtggga ttggtgcggg
     1621 cggtgaccgc ctttcccagt agtccttgaa cgcctctcgc caggccacaa actgagaaaa
     1681 aggcggcaca tggtaacgcg tacccgcata aaggagtccg gcgaaaacga gagcggccaa
     1741 cagttcaaga cgaccacgaa cgagcccgag agtgccacgc ttgagatagc cgagtaacat
     1801 cttccaattg aaaaggaggt gaagaatact gaacaccacg aagcagatgg ttgcattgat
     1861 atggaaggcc gcccaagact ccttgtccca acccaatacc gtccactgtc cccaattggc
     1921 aacccgtcca cggggggaac tataaagaat ggcaccggac agggtaacga ttaggaacaa
     1981 tacaagaagc gacagagagg taaacatttg gaatcgaaag agcgggtcgc gcttgtgaac
     2041 ttctttaggg tgattcatgg ccgtcgtgtc caaagacata gcagagtcct tttccagttt
     2101 ggtgttttct gatcaacaga gccccttccc cgcatcattg tagcaaacga gataccacat
     2161 caagtcgaat cgtaagttat gatttaatat tatgttatga gtacccgatt aagtacggtc
     2221 ttctgcctat gcaacaattg cgcaccgctc aacctaccag tggctactct tgtcatagat
     2281 tcatcgggtg aaaatagaca gccacatctt gctcgcgggg ctccgcacga cagacgaccc
     2341 aagtacgagg caggttgaac agtcgtctct ttctcattac attcggtgta ctgttagcta
     2401 tcacatgtgg attcctgcaa tcgattcgaa ttcacgactt cttgaaagga aggcgctatg
     2461 gttcgattga aatggcttcg atttgtcctg gcggcggttg gattggcatc tatcaacatg
     2521 ggagacgcag cggcacagga agatagtccc ccaaacatcg tcttcatcta tgccgatgat
     2581 ttgggatacg gcgacatcgg ctgttatggg gccacgcgga ttcctacacc caatttggat
     2641 cgtcttgcca aagaggggct tctctttcgg gatgcccatt cgcctgccgc aacctgcacg
     2701 ccatctcgtt ttgccctgat gaccggacaa tatgcctttc gcaaaccggg caccggtgtt
     2761 cttccgggga acgccgacct gatcatcgaa cctggccagt tgactcttcc tctgatgctc
     2821 aagcgagcag gttacataac ttctgtcgtg ggcaaatggc acctgggact cggtcggaat
     2881 ggttgtgatt ggaatgggac aatcgcgcct gggccattgg agattggctt cgattatgca
     2941 ttcattattc cggctactgg tgatcgtgtt ccctgcgtgt atgttgaaaa ccatcacgta
     3001 gttggcctcg atcccaacga tccgattcag gtcagttacg gtgaaaagat cggtgatgaa
     3061 cccacagggc gcgaacatcc ggagcttctc aagtatcgtt tcagccacgg acacgatggt
     3121 accattatca atggcatcag tcgaatcggt tttatgaaag gtggcaagag tgctcgttgg
     3181 gttgatgaag acatggccga cgttatcaca caaaaggccg tgtcatttat tgaatcgcac
     3241 aaagatcagc gattcttctt gtatttcgcc acgcacgata ttcatgtccc ccgtttacct
     3301 catcccaggt ttgtgggaaa gagccaatg
//
LOCUS       DAOPTR010000374         3328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22856_length_3328_cov_3.947449, whole genome shotgun sequence.
ACCESSION   DAOPTR010000374 DAOPTR010000000
VERSION     DAOPTR010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22856_length_3328_cov_3.947449"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="PKI05_08075"
     CDS             <1..1097
                     /locus_tag="PKI05_08075"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA52200.1"
                     /translation="IRPARLEIAGKLIAEMRDGQQPATYGDTPTDYRRMLERDDLEAV
                     LIATPMQWHAVMAVDALRAGKHVLSEVAATMTVQECWDLVNAVEETGKIYMLAENCCF
                     WRPVLAIGQMIAAGLFGQLTFAECGYVHDCRALAFEADGTLTWRGEIKRDHRGNWYST
                     HSLGPVAKWLGINKGDRLVSLVAAETGRAAMRYYVEQRFPEGHPARQIDFRAGDSTTV
                     LIKTAKEVLIDVRYDVMSARPHPTTVYYTIQGTKAVYDSRIDSVWIEGRSKAKAWEPA
                     GPYVEEFDNPLWKEHAEKAQKTGHSGGDYFVIQQFAEAVRKGGPIPVDVYDAAAWSSV
                     MPLSEQSLNEGGKPVEIPDFTRGKWEQIAS"
     gene            1118..2530
                     /locus_tag="PKI05_08080"
     CDS             1118..2530
                     /locus_tag="PKI05_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52201.1"
                     /translation="MKFRRQFFVIMAVMATGWFSAVVSRGEESLPYVRISPRDPRYFE
                     LSDGKPYIPIGLNMIRPPTDSLDGMEVWFSKLSAEGGNFVRIWLSSPFFDVEHERSGV
                     FDEERAKRIDGLLALAKKYGIRLKLCTEHFRHLGEGTQAWAAKPQHLIANGGPAKDTA
                     DFFLGEAGRAQYKRKLAWYAARYGSDPIIFGWELWNEMDAVRVNVWEPWTAEMLPELH
                     RLFPQNLAMQSLGSYDHENKRERYRRLCELPGNDVLQVHRYLDLGAAWKICHGPVAVF
                     AAEAIKDLRSFGIQKPILLAESGAVEPSHSGPFKLYEKDKDGIILHDVLFAPFFAGAA
                     GPGHIWHWDVYVDKNNLWWHFGRFAEVVRDLDPPAEHFQPVELEHPRLYVYALKGTGK
                     TLIWCRDRENAWQTELAEGKPPATLHGIKLELPPDFRVQESKTVRFYDPWKNAWEVGK
                     TESPLLLPDFRRSLVIRIEY"
     gene            2745..>3328
                     /locus_tag="PKI05_08085"
     CDS             2745..>3328
                     /locus_tag="PKI05_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52202.1"
                     /translation="MTISVFWKVCLVWGIILVTFYSGIGALDQRFASVSVAEEQASQP
                     RSAAPWSVLQRVLNNIFASEDAFPVLPQRLEESEASSAPVYEAGTYDRGELRIVNQVP
                     VLILRGKPEEIAIQESKLTSDAIANLVIAPQEFLRQLNYPGGWEGLIARGRNFWRYVP
                     ENYRRELVTMAEAAGLNHDELFAIGRKFNFRWEPA"
BASE COUNT          743 a          865 c          996 g          724 t
ORIGIN      
        1 atattcgtcc ggcacgacta gagattgccg gcaaacttat cgccgaaatg cgggatgggc
       61 agcaaccagc gacttatggc gacacgccca ccgattatcg ccggatgcta gagcgcgatg
      121 acctggaggc ggtgcttatc gctacgccca tgcaatggca cgccgtgatg gcggtggatg
      181 cccttcgggc agggaagcac gtgctcagcg aagtggccgc caccatgacg gtccaggagt
      241 gttgggacct cgtcaatgcc gtggaagaaa cgggcaagat ttatatgctg gccgaaaatt
      301 gctgcttctg gcgacctgtg ctcgccatcg gtcagatgat tgcggcgggc ctctttggac
      361 agctcacgtt cgcggaatgc ggatatgtcc acgactgccg ggccttggcc ttcgaggccg
      421 atggcacgct cacttggcgg ggagaaatca agcgcgacca cagagggaac tggtattcca
      481 cgcactccct tggtccggtg gccaagtggc tgggcattaa caaaggcgat cgtctagtca
      541 gcttggtggc tgctgaaacg ggccgggcgg caatgcggta ttatgtggag cagcggtttc
      601 ccgagggaca tcctgctcgg caaatcgact ttcgggcggg ggactcgacc acagtgctta
      661 tcaaaacagc gaaggaagtg ctgatcgatg tgagatacga cgtcatgagt gctcggccgc
      721 accctaccac cgtttattac acaatccagg gaacaaaggc ggtttacgat tcgcggatcg
      781 actccgtctg gatagaaggg cgctccaagg ccaaggcctg ggaacctgcc gggccatatg
      841 tcgaagagtt tgataatcct ctgtggaaag aacatgcgga aaaagcccaa aagaccggcc
      901 acagtggcgg cgattacttt gtcattcagc agttcgcgga ggccgtccgc aaaggcgggc
      961 cgatcccagt cgatgtctat gacgccgccg cttggagcag cgttatgccg ctttctgagc
     1021 agtctctcaa tgaaggcggc aaacccgtag aaatccccga ctttacccgt ggaaaatggg
     1081 agcagatcgc aagctagcgg acgaggagaa tcccacgatg aagttccgaa ggcagttttt
     1141 tgtcatcatg gctgttatgg cgacagggtg gttctctgcc gtcgtgtccc gaggagaaga
     1201 gtcgctgccc tatgttcgca ttagcccgcg ggatccccgc tactttgagc tttccgacgg
     1261 caagccctac atccccatcg ggctgaatat gattcgcccg ccgaccgata gtctggacgg
     1321 gatggaagtg tggttttcca agctctccgc cgagggaggc aatttcgtgc ggatttggct
     1381 aagcagtccg ttttttgatg tggaacatga acgaagcggg gtattcgatg aagagcgagc
     1441 caaacgtatc gacggcctgc tggccctggc caaaaagtat gggattcgcc tcaagttgtg
     1501 tacggaacac tttcggcacc tcggtgaagg gacgcaggct tgggcggcta aaccgcagca
     1561 cctaatcgcc aacggtgggc cggccaaaga tacggccgac tttttcctgg gcgaagcggg
     1621 gcgtgcccaa tataaacgaa agctggcctg gtacgccgct cgctatggca gtgatccgat
     1681 catcttcggg tgggaattat ggaatgagat ggacgccgtc agggtcaacg tatgggaacc
     1741 gtggacggcc gagatgcttc cggagcttca ccgccttttc ccgcaaaacc tggccatgca
     1801 aagtctggga agttacgatc acgaaaacaa acgtgaacgc taccggcggc tatgtgaact
     1861 tcccggcaac gatgtcctgc aagtgcatcg ctatcttgac ctcggggcag cctggaaaat
     1921 ctgtcacggc ccagtcgccg tcttcgctgc ggaggcgatc aaagacctcc gctcgtttgg
     1981 cattcaaaag ccgatccttc tcgcagaaag cggggctgtc gagccaagcc acagcggtcc
     2041 cttcaaactt tatgaaaagg acaaagacgg tatcatcctt cacgacgttc tttttgcccc
     2101 gttttttgcg ggcgccgcag gaccaggcca catttggcac tgggatgttt atgttgataa
     2161 aaacaatctg tggtggcatt ttggccgatt tgccgaggtc gtccgcgatc ttgatccccc
     2221 agccgagcac tttcaaccgg tggagctgga acacccgcgg ctctacgtct acgccctcaa
     2281 gggcactgga aaaacactca tctggtgtcg agatcgggag aacgcctggc aaacggaact
     2341 ggccgaggga aaaccgccgg caacccttca cgggataaag ctggaacttc cgcctgactt
     2401 tcgcgtccaa gagagcaaaa ccgtgcggtt ttatgatccg tggaaaaacg cctgggaagt
     2461 gggcaaaacc gagtcgcccc ttctcctccc ggacttccgc agatcactgg tgattcgcat
     2521 tgagtattga gtggttttgg gtaagatttt cacgaatggg ttgagacgga gagtcccacc
     2581 cggcggcatt gtatattgca cggcctattt gccgcattga aaaatctcgt ctaggatgga
     2641 caatgcaggg cgcggcatac ggttttcggt ctcgaagtaa gggttttgtg tgacactact
     2701 ctaagagcaa gggtcagcga atgatgtcca gcaagggagc tcgagtgacg atttctgtgt
     2761 tttggaaggt ttgtcttgtc tggggaatta tcctggttac cttctattcg ggaatcggag
     2821 cgcttgacca gcgtttcgcg tcggtgtctg ttgccgaaga gcaagcaagc cagcctcggt
     2881 cggcagcacc ctggtcagtc ttgcagcgtg tgctgaataa catctttgcc tcggaagacg
     2941 cttttccggt actaccgcaa agacttgaag agtctgaggc ttcgtcggcc ccagtttatg
     3001 aagcgggaac atatgaccgg ggcgagttgc gtatcgtgaa tcaggttccg gtcttgattt
     3061 tgcgtggaaa accagaagaa attgccatcc aggagtcaaa actaacatcc gacgccatag
     3121 cgaatttagt cattgcccct caagagttcc tccgccagtt gaactatccc gggggctggg
     3181 aggggcttat cgcgcgagga cggaatttct ggagatatgt gccggagaac tatcggcggg
     3241 aactggtgac tatggccgag gcggctggtt tgaatcacga cgagcttttt gccatcgggc
     3301 gtaagttcaa ttttcgatgg gaaccagc
//
LOCUS       DAOPTR010000375         3327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22864_length_3327_cov_3.143643, whole genome shotgun sequence.
ACCESSION   DAOPTR010000375 DAOPTR010000000
VERSION     DAOPTR010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22864_length_3327_cov_3.143643"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..902)
                     /locus_tag="PKI05_08090"
     CDS             complement(195..902)
                     /locus_tag="PKI05_08090"
                     /inference="COORDINATES: protein motif:HMM:NF020771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HOA52203.1"
                     /translation="MSVEVDDTKSRLLTAAGELFAERGFEGATARQICERAGVNLAAI
                     NYHFKSKEQLYIEAVKAAAPVFHAELAELEVELDQIQEERRRDAAAALLKKYIYLLMH
                     RMVGIHVPDWKVKLIMREMLEPTGGCQEFFRQHVQRGFEILMRIVNLLLPEDTPEHRR
                     VQIGLSIVSQCLHYRMARDVIQFLIPRRDDPAFSPDSLAEHIVEFVFSALGLSPPFVR
                     QWERKDCEYREGAHLSG"
     gene            complement(954..1199)
                     /locus_tag="PKI05_08095"
     CDS             complement(954..1199)
                     /locus_tag="PKI05_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52204.1"
                     /translation="MLSLRVGVFNCREEQARRSQRKKRGRPLEDGLREMGSFDCARHQ
                     PVRWIAKESPALRVDELMRRTCSSIFFRRIIVFKSFV"
     gene            1269..>3327
                     /locus_tag="PKI05_08100"
     CDS             1269..>3327
                     /locus_tag="PKI05_08100"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOA52205.1"
                     /translation="MKIAVASPTLSVHYFVVISAGLMMTPLNYGHAHANEQSSAVVGR
                     QFVFESAPFKSCHASTIAETEAGLAVAWFGGDREGAENVGIWLCRLTQDGWSAPVEIA
                     TGVQEDGRRYPCWNPVLFQPKEGPLVLFYKVGPRPSSWWGMMMSSQDGGRTWGPPKRL
                     PDGFLGPIKNKPIELADGTWLCPSSTETPESPSRWQIHFEFTRDRGVTWEKRIPPQGA
                     EPLDAIQPSVLRLSNDRLVAVGRTRQRRVFFTASYDNGQTWTPVQKTTVPNPNAGIDA
                     VTLRDGRQLLVYNHATTGRSPLNVALSHDGVNWVPVLVLEDQPGEFSYPSVIQTRDGL
                     VHIVYTWKRQRICHVIIDPEKLTLEHQEPTSQPQAGHGSAPRKEKTPNVIIVITDDQG
                     LGDLSCQGNPILWTPHLDRLAAEGVRLTDFHVAPVCTPTRGQLMTCQDALRNGARSVP
                     AGTNMIWRHVPTMAEIFRASGYRTGHFGKWHLGDHYPDRPIDRGFEKSVWFGGWGVSS
                     DVEFDNDCLKIRYRDGDQVSVADRYCTDFWFDEALRWMEECRKSQQPFFCYIATNAPH
                     GPLWAQDKDAAPYRSRVPAAVANFFGMIANIDENMGRLENWLIDSGLREDTIVIFMTD
                     NGGTAGRKVFNAGLREGKGSYYEGGHRVPCFIRWPNGGIQGPRFISQPTQVQDILPTL
                     VDLC"
BASE COUNT          742 a          935 c          898 g          752 t
ORIGIN      
        1 aatgacaaca aatggagcca cgacattgaa aatgacctcg aaccaagatc gttccgcaac
       61 ggcccgataa gacatgcaac caccctttcc gcttattcct cggcagaaac ctttcggtct
      121 tcaccgaatg atgtttatct ccatgtccag taattgggtt ttcggtctct cacactggcg
      181 attgaccgcg tccatcatcc ggacagatgg gcaccttctc tatattcgca atccttacgt
      241 tcccattgcc gaacgaaagg cggcgacaat cctagggcgg agaagacaaa ctccacgata
      301 tgctctgcca aactgtccgg gctaaacgcc ggatcatctc gacgcggaat caaaaactgg
      361 atcacatcgc gtgccatgcg ataatgaagg cactgcgaaa cgatgctcaa accgatctga
      421 acccggcgat gctccggcgt gtcctctggc aaaaggaggt tcacaattct cattaagatc
      481 tcaaaccctc gctggacatg ctgccgaaag aactcctgac acccccctgt cggctccagc
      541 atttcccgca taatcagctt gaccttccag tcagggacgt gaatcccgac catacggtgc
      601 attaagagat agatgtattt tttgaggagc gctgccgccg cgtcccgccg cctttcttcc
      661 tggatctgat cgagttcgac ttctagctca gccagctccg cgtgaaacac cggtgcggcc
      721 gccttgacgg cctcaatgta aagctgttcc ttcgacttga agtggtaatt gatcgcggcg
      781 agattgacgc ccgccctttc gcaaatttgg cgggcggtgg ccccctcaaa ccctcgctcg
      841 gcaaatagtt ccccggcagc cgtcaacagc cgagacttcg tgtcatccac ttccacagac
      901 attatgggcc ctcccacagt atcactacct ggcctttcaa acaaacaatt gtattaaaca
      961 aacgatttaa agacaatgat gcggcggaaa aaaatcgaag agcaggtacg acgcatcagc
     1021 tcatcgacac gcagggcggg tgattctttc gcaatccagc ggaccggttg atggcgcgca
     1081 caatcaaacg aacccatttc ccgcaatccg tcttccaggg gacgacctct tttctttcgc
     1141 tggctccgcc gcgcttgctc ttcccgacaa ttgaagaccc caacgcgaag cgatagcatg
     1201 gtgggtaccg ctggatttgg agctcttgcg gggcaccccg aaaaagacga gctgagggag
     1261 gtttacccat gaagattgcc gtggcgtcgc cgacgttgtc cgttcactac ttcgtagtca
     1321 tcagcgcggg gttgatgatg acaccgttaa actacggcca tgctcacgcc aatgagcagt
     1381 cttcggcggt tgtcggtcgc cagttcgttt tcgagtcggc accgtttaag agctgccatg
     1441 cttccacgat tgccgaaacc gaggctgggt tggctgttgc ctggtttggc ggggaccgcg
     1501 agggagcaga gaacgtcggc atctggctgt gccgactcac acaagatggc tggagtgctc
     1561 cggtcgaaat tgcaactggt gttcaagaag atggtcgccg ctacccctgt tggaatcccg
     1621 ttctgtttca accgaaggag ggaccactcg tcctctttta caaggttggt cctcggccta
     1681 gcagttggtg ggggatgatg atgagtagtc aggacggagg acgtacctgg ggcccgccga
     1741 aacgactgcc cgacggtttt ctgggcccta ttaagaataa gcccatcgag ttggctgatg
     1801 gcacatggtt atgcccttcc agcacagaga caccggagtc gcccagccgt tggcaaatcc
     1861 attttgagtt cacccgagat cggggcgtaa cttgggaaaa gcgcatcccc cctcagggag
     1921 ctgaacccct ggatgccatt cagccttccg tattgcgact atcaaatgat cgtcttgtag
     1981 ccgtggggag gacacggcag cgacgggtct ttttcacggc ttcgtatgac aatggtcaga
     2041 catggacgcc cgtccaaaag accaccgtgc caaatcctaa cgccgggatc gatgccgtta
     2101 cgcttcgcga tggacggcag cttttggttt ataaccacgc gacaacaggg cgatcgccgc
     2161 ttaatgtcgc gctgtctcat gatggagtca actgggtgcc agtattagtg ctggaggacc
     2221 aacccggcga atttagctat ccttcggtga ttcagacccg cgatggtttg gtccacatcg
     2281 tttatacttg gaaacgtcaa cgcatttgcc atgtgatcat cgaccctgaa aagttgacgc
     2341 tcgagcatca agaaccaacg tcgcagcccc aagctggcca cggatcggcc ccccgtaaag
     2401 agaaaacacc gaacgtgatt atcgtcatca ccgatgatca ggggttaggc gatctttctt
     2461 gccagggaaa cccgattctt tggactcccc acctcgaccg gcttgccgct gagggtgttc
     2521 gcttaaccga ttttcacgtg gctccggtgt gcacaccgac gcgggggcaa ctgatgacgt
     2581 gtcaggatgc ccttcgcaat ggtgcccgta gtgtccccgc tggtaccaac atgatttggc
     2641 gtcacgtgcc gacaatggcc gagatattcc gggccagcgg ctatcggaca ggacatttcg
     2701 gtaagtggca cctcggggac cattatcctg atcggcctat agatcgtggt tttgaaaaat
     2761 cggtttggtt tgggggatgg ggtgtttctt ccgatgtgga gttcgacaac gattgtctca
     2821 aaattcggta ccgtgatggt gatcaggttt ccgtggccga ccgttattgc accgactttt
     2881 ggttcgatga ggcccttcgc tggatggagg agtgccgaaa atcacaacaa cctttctttt
     2941 gttacatcgc cacaaatgcc ccgcatgggc cgctctgggc ccaagacaag gatgcggctc
     3001 cgtatcgcag ccgcgtgcct gccgcagtgg ccaacttttt tggcatgatc gccaacatcg
     3061 acgagaacat ggggcggctc gagaattggc tcatcgactc cgggctacgc gaggacacca
     3121 tcgtgatctt catgacagac aatggtggga cggcaggacg caaggttttc aacgcaggtt
     3181 tgcgggaagg aaaaggcagt tactacgagg gtggtcatcg cgtcccctgc tttatccgtt
     3241 ggcctaacgg aggaattcaa ggccctcgct tcatcagtca gccgacacag gtccaggata
     3301 tcctgccaac tttggtggat ctgtgcg
//
LOCUS       DAOPTR010000376         3319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22934_length_3319_cov_3.735907, whole genome shotgun sequence.
ACCESSION   DAOPTR010000376 DAOPTR010000000
VERSION     DAOPTR010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22934_length_3319_cov_3.735907"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="PKI05_08105"
     CDS             complement(<1..1197)
                     /locus_tag="PKI05_08105"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOA52206.1"
                     /translation="MVLAMVMACVAMPTFIEAEDVKIMTVSTPDAGLGIGHYPANQPP
                     LMPSHLAKLRVGAVRAEGWLLTQLKLEADGFIGHLHELSRFLADEGNAWLNPAGEGDR
                     SFWEELPYWLKGYGDLAYLLGDEKMIAEARRWIEATLASQREDGYFGPRRNLEMVNTP
                     REKKPDHWPNMVMLHALQSYHEFTGDPRVLELMKRYFRWQLSQPDEDFLLPFWQNQRA
                     SDNLVSVYWLYNRSPEPWLLELGAKIHRCMARWDTGVVNWHGVNMAQCFRAPGIYYQQ
                     AGDRKLLQMAYANYEEMREKYGQVPGGLYGADENCRPGHTDPRQAAETCAMVEMMYSC
                     ELLTAISGDVIWADRCEDVAFNSLPASMTADLKALRYLTAPNLAVSDGKNHAPGFQNA
                     GPMLLFD"
     gene            1649..2848
                     /locus_tag="PKI05_08110"
     CDS             1649..2848
                     /locus_tag="PKI05_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HOA52207.1"
                     /translation="MNRLVCFTGLLMILFVAWSLSENRRAMNWRLIISGLVMQWIIAL
                     IMLKTGVGQAAFFGGRILIERLQACVNQGTSFVFGPEAQHHQVAFVVLPLIIFVSSLF
                     AVLFYLGILQWLVKIMAKVMVWVMDVSGAESLAAAANIYVGMTEAPLLIRPYLSTMTR
                     SELMAMMSSGMATIAGTVMAAYVAMGVDAGHIMTASLMSAPASLVVAKIMVPELELSP
                     TKGIVRVAVPREDVNLLDAACRGASDGLMLTLNVAAMLIAFIALIAFVNWILGLFGDV
                     GNQPLTLERITGYLFVPFAWAMGIEWKDIPTIARLLGLRTVLNEFIAYQELTSIKETL
                     SPRSFIIASYAICGFANFGSIGVMIGGIGRLVPERRSELAILGFRSMVAGTLACYMTA
                     CIAGILL"
     gene            complement(2896..>3319)
                     /locus_tag="PKI05_08115"
     CDS             complement(2896..>3319)
                     /locus_tag="PKI05_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52208.1"
                     /translation="KVSFKAFPVKVTITYHGRPLEGADVRLHPLDPNGRGAQGKTDAS
                     GVATMRSFGEGGAMAEGAMAGKYKVLVSKSVSEVEIPATPEGPDYDRMAAQEARADPY
                     ASSSKPLLPLKYSSWEQSPFECEVTEAGPNEFTFDIPD"
BASE COUNT          696 a          951 c          884 g          788 t
ORIGIN      
        1 gtcaaaaagc aacattggtc cagcgttctg gaatcccggt gcatggttct ttccgtccga
       61 caccgccaag ttaggagcgg tcaggtaccg caaagcctta aggtccgccg tcatcgaggc
      121 cggcagcgag ttaaaggcca catcttcaca ccgatcggcc cagatgacat cgccggaaat
      181 cgcagtcagc agctcgcatg aatacatcat ctccaccatg gcacaggtct cggccgcctg
      241 acgaggatcg gtatgccctg gccgacaatt ctcatccgct ccatacagac cacccgggac
      301 ctggccgtac ttttctctca tctcctcgta attcgcataa gccatttgca ggagtttgcg
      361 gtcgcctgcc tgctgatagt agatccccgg tgcgcggaag cattgagcca tattcacccc
      421 gtgccaattc accacgcccg tgtcccatcg cgccatacag cgatgaatct tggctcccaa
      481 ctccagcagc catggttcgg gcgaacgatt gtaaagccaa tagacgctga cgaggttgtc
      541 gctcgcccgc tggttttgcc aaaacggaag cagaaaatct tcatccggct gcgacaattg
      601 ccagcgaaaa tagcgtttca ttagttccag gacgcgagga tcacctgtaa actcatgata
      661 tgattgcaac gcatgaagca tgaccatgtt gggccagtga tcgggctttt tctcccgcgg
      721 agtgttgacc atctcaagat tcctccgcgg accgaaatag ccgtcttccc gttggctagc
      781 aagtgtggcc tcaatccacc ggcgggcctc ggcaatcatc ttctcatcgc ccagcagata
      841 ggccaggtcg ccgtaaccct tcagccaata gggcaactcc tcccagaagc ttcgatcccc
      901 ttcccctgcg gggttgagcc aggcattacc ctcgtccgca agaaaacgac tcaactcatg
      961 aagatgaccg ataaagccat cggcttcgag cttcaattgc gttagaagcc acccttccgc
     1021 gcgaacagcc ccaacacgta gttttgctag atggcttggc ataagtggcg gctggttggc
     1081 cggataatgc ccaataccga ggcccgcatc gggcgtggaa acagtcatga tcttcacgtc
     1141 ttccgcctct ataaacgttg gcatggccac acatgccatt accatggcca gtaccatccc
     1201 catgttcctt agcgcgcttc gccaatagct catgagcatg agtcattccc ccgttaaata
     1261 agagtgaccg aatcgacgaa gtccgaacat gcaaattgct tcgattctca acgggtacat
     1321 tgaggacaac acgggctccc cgggaaagcc aaaggttatc tgaggtagcc tgacagcctt
     1381 tcgccgaggt tgattcaatt tgggcgctcc gcgggcagtt gtcaagccgc gcaacgcctg
     1441 aaccacgcat cctttgaaag atggcggctg acgcttatat taccgttctc ggcatgcggc
     1501 aatgaaggaa tcatattcct gaattggtct tacattgact catgcaatga gcttttgccc
     1561 actggtggtt ttctgggttg agaaaacgga cacacggctt gattctcacg aacatggata
     1621 aagtgcgtca gtaaatggag gcttaccgat gaatcgattg gtttgtttca ccgggcttct
     1681 gatgatcctc tttgtggcgt ggagtctttc cgaaaatcgc cgggcgatga actggcggct
     1741 tatcatcagt ggactagtca tgcagtggat cattgccctc attatgttaa aaacgggggt
     1801 cggacaggcg gccttctttg gtggccgtat cctgatcgaa cggctccaag cgtgcgtcaa
     1861 ccagggcacg agctttgtct tcggtcccga ggcccagcat catcaagtag cctttgtcgt
     1921 cttgccacta attatctttg tttcgtccct cttcgcagtg ctgttttatc ttggaatcct
     1981 ccaatggttg gtgaaaatca tggccaaggt gatggtctgg gtcatggatg tctccggtgc
     2041 agaatccctt gccgcagcgg cgaatattta cgtcggcatg acggaagcgc cgctcctcat
     2101 ccggccatat cttagcacca tgacccgttc cgaattaatg gccatgatga gcagcggcat
     2161 ggccaccatc gcagggaccg tcatggcggc ttacgtggcg atgggcgttg atgctggaca
     2221 cattatgacg gcctcactga tgtcggcacc ggcctcgctc gtcgtcgcca agatcatggt
     2281 ccccgagctt gagctctcac cgacaaaagg gatcgttcgc gtggccgtgc ccagggaaga
     2341 tgtcaacctc cttgacgccg cttgccgcgg ggcgagcgat ggtctgatgc tgacactcaa
     2401 cgtggcggcc atgctcatcg cgttcattgc cctcattgcc tttgtcaatt ggatcttggg
     2461 gctgttcggg gatgtgggaa accaaccact gacgctggaa cggatcacgg gttatctgtt
     2521 tgtgcccttc gcctgggcca tgggaatcga gtggaaagat atcccgacca ttgcgagact
     2581 cctcggtctg cggaccgtcc tcaacgaatt catcgcttac caagagctga caagtatcaa
     2641 ggagacgctt tcgccccggt cattcatcat tgcgtcgtac gccatctgtg gttttgccaa
     2701 ctttggttcg attggtgtga tgattggggg aattggccgc ctcgttcccg aacggcgttc
     2761 ggaattggcc atactcggat ttcggtcgat ggtggccggg acactcgcct gctatatgac
     2821 ggcctgtatc gccgggattc ttctataggc aatttgccac caagacaaac gatccgtacc
     2881 gtaggtctgc gtcggtcagt ctggaatgtc gaaggtgaac tcgttaggtc ctgcctctgt
     2941 gacctcacat tcaaaggggc tttgttccca cgagctgtat ttgagaggga gcagtggctt
     3001 agaactagag gcgtatgggt cggcccttgc ttcctgggcc gccatgcggt cgtagtccgg
     3061 cccttccggc gtcgcaggga tttcgacctc ggagacggac ttggacacta acactttgta
     3121 cttgccggcc atcgctccct cggccatggc tccgccctca ccaaaacttc tcatcgttgc
     3181 cactcccgaa gcgtccgtct ttccctgagc gcctcggcca tttggatcta agggatggag
     3241 acgtacatcg gccccctcca ggggccgccc atggtaggta atcgtaacct tcacggggaa
     3301 ggccttgaag gagactttt
//
LOCUS       DAOPTR010000377         3319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22939_length_3319_cov_3.061581, whole genome shotgun sequence.
ACCESSION   DAOPTR010000377 DAOPTR010000000
VERSION     DAOPTR010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22939_length_3319_cov_3.061581"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PKI05_08120"
     CDS             <1..321
                     /locus_tag="PKI05_08120"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOA52209.1"
                     /translation="KSGFHQGVDIACPEGTPVYSVIAGRVVSARSSGVYGKCIVIQSG
                     EMSTYYGHLSGYLVAEGDKVEQGQVIGLSGNTGVSTGPHLHFEVRMNQTPCDPGVWLS
                     PPAQ"
     gene            337..1644
                     /locus_tag="PKI05_08125"
     CDS             337..1644
                     /locus_tag="PKI05_08125"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HOA52210.1"
                     /translation="MNSFVSIDLLKPHPKNKEYFSPPDAGEYEAVKQSIATHGIRDPI
                     KILPDYTVVAGHVRLAIARELGIERVPVQVWDVSGEDAEYLLVADNEERRVCRDPVKK
                     AKRAKFLKEYWGIRRGGNRRSKPQNAGLKNLNDVAETLGESSDSLQRLLKLNDLIPPL
                     QELVSGGQLGQTAAYSLAFLPPEEQETLLTTLGETGVCGLSTKEAQDLRREFDSLRRE
                     KEALETRLAEAEKKLAGAGREVVYKTDPVLEMEIEETRKNAAEILKEKERLETQLVTI
                     LREKEKREIEARTATDEAANLRRMLDHARAELKKEKERPKPPQWSPEHIEFQNLIQEA
                     TREAAGLATALSHVLERHRERLVAAARVRGTSSESLRDMAEVLGDSLLFSAFDSSMNV
                     VLSKIAEIQGVLEPGRPKMHVIRSEEKRGGSADDTSKPAKTKA"
     gene            1610..2014
                     /locus_tag="PKI05_08130"
     CDS             1610..2014
                     /locus_tag="PKI05_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52211.1"
                     /translation="MTQANQRKQKRKKLSKAEELLALLELSDGAAPTKEVAVALYSED
                     TTDIRQKVRRLARTLRSWGYRVYGFGGIYKLCTQPQELAVVFERNTKLSRGAVLSADD
                     VAEGIRDLGDPEMAASARNNLKTMLMELTEVL"
     gene            2035..>3319
                     /locus_tag="PKI05_08135"
     CDS             2035..>3319
                     /locus_tag="PKI05_08135"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52212.1"
                     /translation="MRLIAITSPATDAGKTFIATGLAEGLARRGIKTLFVDLDLAVGD
                     SIRVFGLADEARQPHPTVASWRDFPDPWRSSLTGPGGVVVIPRPENNGESVAEDGLTE
                     LIAQAESTGIEAVVMDLGADVRAPFWDGIIERADVSFLVLDTDGKALDRARRFLAASM
                     QPPEGWVLVINQRTPRGGYRQDEMVRNLGAASVTRSVVTIPFIPEKKRPTIRLPVDIP
                     SAGELAEIAMGRPLVELKGGKTSKKSFLARIFSRKASPEIQNDEEPFVTPFRENKGFE
                     GISSPMPTEAISNRKALLLGRAQSLAATLENLGWDTTFYEHDDYDVALADPATFKRVP
                     TEKQTLIIGAGMDLFFSSDQLAHESAIPVANENAVVAILQERFGGALIRRQSSEHSIA
                     LSIAKKEAAGVVVGFYSGAQGYQGKTVLALNTAALL"
BASE COUNT          857 a          775 c         1005 g          682 t
ORIGIN      
        1 aaatcggggt ttcaccaagg cgtcgacata gcttgccctg aaggaacccc ggtttacagc
       61 gttattgcgg gtcgggtagt cagtgcgcgt tcgtccggcg tctacggaaa atgcatcgtt
      121 atccaatcgg gcgagatgtc aacttactac gggcatttaa gcgggtatct ggtcgccgag
      181 ggggataagg tcgagcaagg tcaggtaatc gggttatcgg gtaatacagg ggtgtctacc
      241 gggccgcatc ttcatttcga ggtccgcatg aaccagaccc cttgcgaccc cggtgtctgg
      301 ttgtcgccgc cagcacaata ggaaaggaga atcggtttga atagttttgt ttccattgat
      361 ttgttgaaac cgcacccgaa aaacaaagaa tacttttccc cgccggatgc gggggaatac
      421 gaggcggtca aacaaagcat cgccacccac ggtatccgcg accctattaa aatcctgccc
      481 gattacactg tggtcgccgg gcatgtcagg ctcgcgattg ctcgggaatt gggcatcgaa
      541 agggttccag tccaagtatg ggacgtgtcc ggggaggatg ccgagtattt gctggtagcg
      601 gataacgagg aacgtcgagt atgccgtgat ccagttaaga aagcaaaaag agcgaagttt
      661 ttgaaggagt attggggtat taggcgagga ggtaacagga gatcaaaacc gcaaaatgcg
      721 ggtttgaaaa acctaaatga cgtggccgaa acccttggcg aaagtagtga ttcactacaa
      781 cgtctcctca agctcaacga ccttattcct cccttgcagg aactcgtgtc cggcggccaa
      841 cttgggcaga ccgccgccta ttctctcgcg ttcctcccgc cggaggaaca ggagaccttg
      901 ctcacaaccc tcggagaaac gggagtgtgc ggcctttcaa ccaaagaggc ccaggatttg
      961 cgcagggaat ttgattcctt acgccgcgaa aaagaagcgc tggaaacccg cttggccgag
     1021 gcggaaaaga aacttgccgg ggcggggcga gaagtggttt acaagaccga ccctgtcttg
     1081 gaaatggaaa tcgaggaaac ccggaaaaac gcggcggaaa tcctgaaaga aaaggagcgg
     1141 ttggaaaccc aactcgtgac aatccttcgt gaaaaggaga agcgtgaaat cgaggcccgt
     1201 accgcgaccg atgaggcggc caatcttcgc cggatgctgg atcacgctcg ggccgaactg
     1261 aaaaaggaaa aggaacggcc caaaccgcct caatggtcac cggaacacat tgaattccaa
     1321 aacctgatac aagaggcgac cagagaggcc gcaggtttag cgaccgcgtt gtcccatgtg
     1381 ctcgaacgac accgagaacg gttggtcgcc gccgcgaggg ttcgcgggac ctcgtcggaa
     1441 tccttgcggg atatggccga ggttctgggg gattcattgt tgttcagcgc atttgattca
     1501 agtatgaatg tggtcttatc caagatagct gagatccagg gagtgctgga accggggaga
     1561 cccaaaatgc acgttattcg aagtgaagaa aaacggggag gaagcgcaga tgacacaagc
     1621 aaaccagcga aaacaaaagc gtaaaaagct atcgaaagca gaggaactgt tggcgttatt
     1681 ggaattatcg gacggggccg cgccgaccaa agaagtggcg gtggcgttgt atagcgagga
     1741 taccaccgac atacgccaga aagtgcgtag gttggcgcgg acgttgcgtt cgtggggata
     1801 ccgggtctac gggttcgggg gtatttacaa actctgtacc caaccccagg aactcgcggt
     1861 agtgttcgaa cgaaatacga aattatcgcg tggggccgtg ctttctgcgg acgatgtggc
     1921 ggagggcatc cgggatttgg gcgacccgga gatggccgcg tcagcgagga ataacttgaa
     1981 aaccatgtta atggaattga ccgaggtatt atagagcgga ggttgtaaca tattatgcga
     2041 ttgatagcga ttacttcccc cgcaacggac gcgggtaaaa cattcatagc gaccggactt
     2101 gcagaggggt tggcacggcg aggcattaag acattgtttg tcgaccttga ccttgcggtc
     2161 ggtgattcca ttagggtatt cggtcttgcg gacgaggcgc gacaaccaca tccaacggta
     2221 gcatcgtggc gggattttcc tgacccgtgg agatcatcgt taacggggcc ggggggagtg
     2281 gtggtcattc ctcgaccgga aaacaacggg gaaagcgtag ccgaggatgg gttgacagaa
     2341 cttattgccc aggccgaaag tacagggatt gaggcggtcg tgatggatct gggggccgac
     2401 gtgcgcgccc ctttctggga cgggatcata gagagggcgg atgtctcgtt tttggttctg
     2461 gataccgacg ggaaagcatt ggatcgggcg cgacgttttc tggctgcgtc catgcaacca
     2521 ccggagggat gggtcttggt catcaatcaa cgtaccccac gcggagggta tcgccaggat
     2581 gaaatggtta gaaatctcgg cgcggccagc gtcacgcgct cggtagttac gattccgttt
     2641 atccctgaaa aaaagagacc taccattcgg ttgcccgtgg atatacctag tgctggtgaa
     2701 ctggctgaaa tagcgatggg gcgaccactt gtggaattga aaggaggtaa aacctcaaaa
     2761 aagagctttt tggcacggat ctttagtcgc aaggcaagtc ctgagataca gaatgatgaa
     2821 gaacccttcg ttaccccgtt cagagagaat aaagggttcg agggaatctc atcaccaatg
     2881 cctacagaag caatcagtaa cagaaaagca ttgttgttgg gtagagcgca atcgctcgcc
     2941 gcaacattgg aaaacttggg ctgggacacc acattctacg aacacgatga ctacgatgtc
     3001 gcacttgcgg acccggcaac attcaagagg gttccgacag aaaaacaaac tcttattatt
     3061 ggggcgggga tggatttgtt tttttcctcc gaccaattag cacatgaatc agcaatcccg
     3121 gtggctaacg aaaatgcggt cgtagcgatt ttgcaggaaa gattcggcgg ggcgttaatt
     3181 cgtcgccaat caagtgaaca tagtatcgcc ctcagcatag ccaagaaaga ggccgccgga
     3241 gttgttgtcg ggttctattc cggggcgcaa ggataccagg ggaaaacggt cctggccctc
     3301 aatacggcgg cgttacttg
//
LOCUS       DAOPTR010000378         3318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22946_length_3318_cov_4.262335, whole genome shotgun sequence.
ACCESSION   DAOPTR010000378 DAOPTR010000000
VERSION     DAOPTR010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22946_length_3318_cov_4.262335"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..1574
                     /locus_tag="PKI05_08140"
     CDS             186..1574
                     /locus_tag="PKI05_08140"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA52213.1"
                     /translation="MLLILLLLIFASGAVSAVALANSWENIGGATKKPKNVLILFSDD
                     QRFDTIRALGNQEIETPNLDRLVHEGFAFTHAFIMGSMQGAVCVPSRAMFLSGQTLWR
                     VPENLSGVPTWPEVFKNHGFVTFGTGKWHNGRESFARSFTQGAAIFFGGMSDHNQVPI
                     FDYDPTGKYAQANVKTGNKFSSELFADACIEFLRGFKGDRPFMAYVAFTAPHDPRTPP
                     DEYRKKYDPADVSVPPNFLPEHPFDNGELRVRDELLTSFPRHPDEIRRHIAEYYGMIT
                     HLDAQIGRILAALAEAGHQDDTLIVFAGDNGLAIGQHGLMGKQNLYEHSQRVPLIIAG
                     AGIPHGRSDALVYLIDVYPTVSELLNVPVPDGVEGQSLVPIIYGKKTSVREAVFGAYR
                     EFQRSVRTDRYKYIRYRVKEETHEQLFDLQSDPWEKHNLADDPAAQEVKRQLQQRLEE
                     YRRELGDPVTEF"
     gene            1644..>2314
                     /locus_tag="PKI05_08145"
     CDS             1644..>2314
                     /locus_tag="PKI05_08145"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOA52214.1"
                     /translation="MEAMVLSDLAHFDLIVPRKIIFGWGRRTALREELPRLGKRVLVI
                     SGSRTLEKAGLFDELQQLVMEGGLDWQIVARIHSEPTVTEVDHVSDAVRALGVRQGDI
                     ILAVGGGSAIDLAKAVAAMVTQDARAPIRDYLENIGRNLKLIQPALPVVAVPTTAGTG
                     AEATRNAVICSHDPPVKKSLRDDRIMPYLAVVDPELTVSNSRAITAAAGMDAITQLIE
                     SYVSVR"
     assembly_gap    2315..2324
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2325..2853
                     /locus_tag="PKI05_08150"
     CDS             <2325..2853
                     /locus_tag="PKI05_08150"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOA52215.1"
                     /translation="RRRPIPQILAPQALRIAFQCLPNAFTNPMDRTAREGMAFAALIS
                     GICLANSGLGMAHGVAAALGALGEVPHGIACAVMLPVALQVNRSVCPSELAYLARIAF
                     DQKFANDEEAIDYLISEVRNLQAKLEIPDRLSKLGIHRELIPAIVNESRGSSMSGNPK
                     QLKDDELMEVLEQIL"
BASE COUNT          754 a          851 c          927 g          776 t
ORIGIN      
        1 ttccccgagt ctttcgtttg gacaaccttc gacacatctt cagtcgatcg cgtccacggc
       61 gataagttag ggagcttgtg agcagcgtcc tggtttggct ttttggggga ggaacgagga
      121 tgacagggtt ttccagcaga aaagttatat ttttcatgtg ttatgaaagg cgccagacga
      181 ataggttgct ccttatttta ttgctgctga tcttcgcatc aggtgctgtg tccgcagtgg
      241 cgttggcgaa ttcgtgggaa aatattggcg gggccacgaa gaagccgaag aacgtcctaa
      301 tcttgttttc cgatgatcag cgattcgaca caattcgcgc gttgggcaat caagagatcg
      361 aaacgccaaa tcttgatcgg cttgttcacg aaggctttgc cttcacgcat gccttcatca
      421 tgggaagtat gcagggggcc gtgtgtgtcc ctagtcgggc gatgttcctc agcgggcaga
      481 cattatggcg ggtaccggag aacctatccg gcgttcccac atggccggag gtcttcaaaa
      541 accatggttt tgttacgttc ggcacgggaa aatggcacaa tggtcgcgag tcgtttgctc
      601 ggagctttac ccagggtgcg gccatctttt tcggcggaat gagcgatcac aatcaggtgc
      661 ctatctttga ctatgatccc acgggaaagt atgcccaggc gaacgttaaa actggcaaca
      721 agttctccag tgagcttttc gccgacgcgt gtatcgagtt tctgcgaggg tttaaggggg
      781 atcggccgtt catggcctat gtggcgttca cagcccccca tgacccgcgg actccacctg
      841 acgaatatcg gaaaaagtac gacccggccg acgtctcagt cccgcccaat tttctccctg
      901 aacatccatt tgacaacggt gaattgcgcg ttcgcgatga gctactgacg tctttccccc
      961 gtcatcctga tgagattcga cgccatatcg ctgagtacta tgggatgatt actcatctgg
     1021 atgcccagat cggccgcatt ttggcggccc ttgctgaagc cggacaccag gacgataccc
     1081 tgatcgtgtt tgcgggtgac aatggacttg ctatagggca acatggcctg atgggcaagc
     1141 agaacctcta tgagcacagc cagcgggttc cgctgataat agcgggggcg ggcattcccc
     1201 acggacgaag cgatgccctt gtctatttga tcgacgtgta tcctacggtg tcggagttgc
     1261 taaatgtgcc agtccctgac ggagttgaag ggcaatcgtt ggtacctatt atctatggca
     1321 agaagaccag tgttcgcgag gcggtgtttg gggcctatcg ggaatttcaa cgatcggtcc
     1381 gaacagacag atataagtac atccgctatc gcgtcaagga ggaaacccat gagcaactat
     1441 tcgatttgca atcggaccca tgggaaaagc acaatctagc cgacgatccc gccgcgcagg
     1501 aagtcaaacg tcaattgcaa caacgtttgg aagagtatcg ccgcgaactc ggtgacccag
     1561 tgacggaatt ctagacattt cggattgccg cgcgaaaaca aatcggttga ttgaccgttg
     1621 atggtgacga agatctactt gtcatggaag ccatggtgct atccgatttg gcccattttg
     1681 acctaatcgt gccccgcaag attatcttcg gctgggggcg acgaacggcg ctccgggagg
     1741 aattgccacg cctgggaaaa cgggttctag tcatttccgg ttctagaacc cttgaaaagg
     1801 ccggtctctt tgatgaatta cagcaactgg taatggaggg tggtctcgat tggcaaatcg
     1861 ttgcccgaat ccattctgag ccaacagtca cagaggtgga ccacgtgtcc gatgccgtgc
     1921 gggctttggg ggtacgccaa ggcgacatca ttctggccgt gggaggtggt tccgccattg
     1981 atttggccaa ggccgtggcg gcgatggtca cacaagatgc gagggctcct attcgagact
     2041 acctcgagaa tattggccgc aacctcaagc tcatccagcc ggctctaccg gtcgtggccg
     2101 ttcccacaac ggcgggcacc ggtgcggaag ccacccgaaa tgctgtcatt tgctcgcacg
     2161 atccacccgt caaaaaaagc ttgcgcgatg atcgaatcat gccatacctg gcagtggtgg
     2221 acccggaact taccgttagc aattctcggg cgatcacggc ggcggcaggc atggatgcca
     2281 tcacccaact aatcgaaagt tacgtcagtg ttcgnnnnnn nnnntcggcg gagaccgatt
     2341 ccccaaatct tggcacccca ggcgctacga attgctttcc aatgtttgcc caatgccttt
     2401 acaaatccaa tggacagaac ggcacgcgaa ggtatggcct ttgccgcctt gatctcggga
     2461 atctgcctgg caaattctgg cctaggaatg gcgcacggtg ttgcggcagc cctgggtgcc
     2521 ctgggcgaag ttccgcatgg aattgcgtgt gctgtgatgc tgccagttgc cttacaggtt
     2581 aaccgaagtg tatgtccctc agaacttgcg tatcttgcta gaattgcctt tgatcagaag
     2641 ttcgccaacg atgaggaggc gatcgattat ctgatcagtg aggtccggaa cctccaggca
     2701 aaactggaaa tccctgaccg gctgagcaag ttgggcattc accgcgagtt gattccggcc
     2761 attgtcaacg aatcccgcgg aagtagtatg agcgggaacc ccaagcagct caaggatgat
     2821 gaacttatgg aagttctcga acagattctt tagttcgcct gtgctccatt ggtgtggggt
     2881 aaacgtcccc tcaaaaggca aacggttggg cgggcttgtt tccttaaggc caccttgcga
     2941 cggcaggttt caaagcttat actttcataa ccacgcaaag gcagtgtccg cggcagcgcg
     3001 atcaccccct tgaaaaaccg gtgaaaattg tatcacttaa aagatactag ctgctaaaag
     3061 gggccatcca ctagtaaacc aggcagctga gactgctcct cagtgtgacg ttgtgcgtta
     3121 gaaccaccca tcgtgcaacg gattcagcaa acgtggaagg acaatacgat gtctcatgat
     3181 catcccacgc ctccggaggt caatcgaaaa ctggtcatcg ctttagtcgt tgtctttctc
     3241 ggctacggcc tgtcattggg gttgggctgg ccgcaggctg cgacgcgaat gattgtggaa
     3301 gcggagtccc atgcctcg
//
LOCUS       DAOPTR010000379         3318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22948_length_3318_cov_3.605884, whole genome shotgun sequence.
ACCESSION   DAOPTR010000379 DAOPTR010000000
VERSION     DAOPTR010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22948_length_3318_cov_3.605884"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1824)
                     /locus_tag="PKI05_08155"
     CDS             complement(<1..1824)
                     /locus_tag="PKI05_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52216.1"
                     /translation="MRSVRDTKVVIAAVVFVFLSAKVLPAEPAKRGPAADGAPAQTSS
                     GSDPMQVAAKEDPAVAAILATNPLTPRERIRAADVLLSLNRPDLAKQMLANVLQSPLD
                     PPTLLALDREFGSATFFRFASHPELDPEGTNLAELVFDAVRNTRCDAERLAMLVNYLC
                     EAPDNCWYRAAKHLIKAESAAVKPLCDALIDPAWRASWPRVYYVLLHFRPASDEALLS
                     LLFTLPEEKQAGIIGGIAALGVTRATPFLLCLAMGPEGARGEQQLAERAIEQLGESLP
                     TPRQAARLLRAEVEAILIESVPLDHEVLTLLRWDASAKQIVAKRVSFEMWRRERAAYL
                     AQHAARLTPDDGELRDLAVCLQLEKEGYLARVEWDSAEIKRSNLSAATAPNIRAMADT
                     VKQILEPGKPTAEDVNRWLNQCLTREWYHASWAVACWMKEYADAGVLVGRDVPALVRC
                     LDAPDPELRFLATDVALSLAAGGSFRGASRVTKELAYFASSEGIAGVLVADPVLRRAA
                     QMTSYLKTIGYARVDTAADGGTLLEMARQSPDYEVILVSAAIQRPGLSLTLQRLRAEP
                     RLARVPVIVYADTDWLPLADSTVRTIPVALSVATPKDPQDLA"
     gene            complement(2059..>3318)
                     /gene="argH"
                     /locus_tag="PKI05_08160"
     CDS             complement(2059..>3318)
                     /gene="argH"
                     /locus_tag="PKI05_08160"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOA52217.1"
                     /translation="MLATVGLLTRDEAAQIVKALEEIREEIDAGNFEFRVGWEDIHMH
                     IERVLIERLGDVGRKLHTARSRNDQVATDFRLWVRDAIDTILPLVRKLQAAFVNRCEA
                     DFDCVLPGYTHLRRAQPVLAPHYWLAYCEKFERDYQRLLDCRKRVNICPLGSAALAGT
                     SLPIDRHMTARELQFDEPSPNSIDATSDRDFAVEFVFDLALLAVHLSHWAEEWIVWSS
                     EEFGFLKLPEGFCTGSSIMPQKINPDVLELIRGKTARVLGHLQQLFVLLKGLPLAYHR
                     DLQEDKPGVFDAYDTVVGCLEVAIPLVELTELNRSRIEESLERGHLDATTLMEYLISR
                     GIPQRTAHSLVGRIVREAMDRQCRLADLPLEVFRMADPSLDANVYSVLGVKNAVQAFR
                     SYGSTCPEEVRKQVERWRERLARDLGA"
BASE COUNT          765 a         1037 c          881 g          635 t
ORIGIN      
        1 tgccaaatct tggggatcct tcggtgtggc gacactcagg gccaccggaa tcgtccgcac
       61 cgtactatcc gccaggggta accaatctgt atcggcataa acgatcaccg ggacgcgagc
      121 caatcgtggc tccgcccgca gtcgctgcaa agtgaggctg agcccaggtc gctggatcgc
      181 cgcggaaacg aggatgactt catagtcagg gctttgccgt gccatctcca agagagtacc
      241 tccgtcggcc gcggtatcca cacgggcata gccgatcgtc ttgaggtaag acgtcatttg
      301 ggcggcacgt ctcaggacag gatcagccac aagaacgccg gctattccct ccgaggaggc
      361 aaaataggcg agttccttgg tgacgcggct ggccccccga aaacttccgc ctgctgctag
      421 gctcaaagct acatctgtcg caagaaagcg gagttctgga tcaggggcgt ccaggcaacg
      481 aaccagcgcc ggaacgtctc ggcccaccaa cacgcccgcg tccgcatact ccttcatcca
      541 gcacgccacg gcccatgaag catggtacca ttctcttgtc agacactgat taagccatcg
      601 gttaacatcc tcagccgtcg gtttccctgg ctccaagatt tgctttaccg tatcggccat
      661 cgcccgaatg ttgggcgccg ttgccgcaga caggttcgac cgctttatct cggccgaatc
      721 ccattctaca cgcgcgaggt aaccctcttt ctccaactgg aggcaaaccg ccagatcacg
      781 gagctcgcca tcgtccggtg ttaatctcgc agcatgttgg gcgagatagg ccgctcgctc
      841 tcgacgccac atctcgaacg acacccgttt cgcaacgatc tgctttgcgg acgcatccca
      901 ccgtaacagt gtgagcacct catgatccaa cggaaccgac tcgatgagta tcgcctcgac
      961 ttccgcccgc aaaagccggg cagcttgccg cggtgtggga agcgactcac ccaactgctc
     1021 tattgccctt tccgccagtt gctgctctcc cctcgctccc tccggaccca tggccaagca
     1081 gaggaggaac ggggtcgcac gggtcacgcc caaagcggca atgcccccaa tgatccccgc
     1141 ctgtttttcc tcgggcaacg tgaaaagtaa tgacaaaagg gcctcatctg aagcggggcg
     1201 aaaatgcagc aaaacataat aaactcgagg ccaagaggcg cgccacgctg ggtcaattaa
     1261 ggcatcacag agcggcttca cggccgcgga ctcggccttg attaagtgct tggccgcccg
     1321 ataccaacaa ttgtcaggtg cctcgcacag ataattcacc agcatggcca accgttcggc
     1381 atcgcaccgt gtgttacgga cggcatcgaa cacgagctcg gcgaggttcg tgccctcggg
     1441 atccaactca ggatggctcg caaagcggaa gaacgtcgcg gaaccgaact cccgatcaag
     1501 cgccaacagc gtgggggggt caagagggct ttgtaaaacg tttgccagca tctgcttggc
     1561 caaatccggg cggttaagac tgagcaacac gtcggctgcc cgaattcttt cccgcggtgt
     1621 caatggattg gtcgccaaaa tcgccgccac agcgggatcc tctttagccg cgacctgcat
     1681 cggatccgat ccggaagacg tttgcgccgg ggcgccatct gcagccgggc cacgcttggc
     1741 tggttccgcc ggcaaaacct tagcagacaa gaaaacaaag accaccgccg cgataaccac
     1801 cttggtatct cggactgacc tcatgacgcg gtcctcttgg tattgcttca cgtgcgcaaa
     1861 cacatagtca ctgagcgtgg aggcccccat ccaccggcaa aagccttggc tttcgaaaac
     1921 ctagcatata ggtccaactg atacggccag aacggccccc tagtacccca tgataattaa
     1981 cgggacaacg attgtcctcg aaaatccaga tatgcctatt aagggtggtg tattaggcaa
     2041 ccgtgtgggg gctacgcact aggccccaag gtctcgcgcc agccgctccc gccacctttc
     2101 aacctgcttg cgaacctcct ccggacacgt tgacccgtaa ctacggaatg cttgcacggc
     2161 gtttttcacc cccaaaacgg aatagacatt tgcatccagc gaaggatcag ccatccggaa
     2221 cacctcgagg ggaaggtccg ccaaacggca ctggcgatcc atggcctcgc gaacgattcg
     2281 ccccaccagc gaatgcgccg ttctctgcgg aatcccgcga gaaatgaggt actccatgag
     2341 cgttgtcgcg tctaagtgtc cccgctccag ggactcctca atgcgggagc ggttcaactc
     2401 ggtcagctcc acaaggggaa tggccacctc caagcaaccc acgaccgtgt cgtaggcgtc
     2461 aaagaccccc ggtttatcct cctgtaaatc ccgatgatac gccaaaggta gccccttcag
     2521 aaggacaaac aactgctgca agtggcccaa gacacgggct gtttttcccc gaatcagttc
     2581 caaaacatcg ggattgatct tttgcggcat gatggaggaa cccgtgcaaa atccttcagg
     2641 caatttcaga aaaccgaatt cctccgagga ccacacaatc cattcctcgg cccaatggct
     2701 gaggtgcact gccagcaagg ccagatcaaa aacaaattcg acagcaaaat cgcggtcgct
     2761 ggttgcatcg atgctgttgg gtgacggctc gtcaaactgc aattcgcggg ccgtcatgtg
     2821 ccgatcaatg ggcaagctag tccctgccaa tgcggcagag ccgagcgggc aaatgttgac
     2881 gcgcttccgg caatccaaaa gacgctgata atcccgctcg aatttctcgc aataagccag
     2941 ccaatagtgg ggtgccagga cgggttgagc ccgacgcagg tgggtatatc ccggcaagac
     3001 acagtcaaag tcggcttcac agcgattgac aaacgcggcc tggagcttgc gaacgagagg
     3061 gaggatcgta tcgatcgcat ctcggaccca gagccggaaa tcggtggcaa cttgatcatt
     3121 acggcttcga gccgtatgga gcttccgccc cacatcgccc aatcgttcga tcaagacccg
     3181 ctcgatgtgc atgtggatgt cttcccaccc cacgcggaac tcaaagtttc ccgcgtcgat
     3241 ttcctctcga atctcttcaa gggccttgac gatttgagcg gcttcatccc gcgtcagcaa
     3301 tccaacggtg gctaacat
//
LOCUS       DAOPTR010000380         3314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22983_length_3314_cov_3.747162, whole genome shotgun sequence.
ACCESSION   DAOPTR010000380 DAOPTR010000000
VERSION     DAOPTR010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22983_length_3314_cov_3.747162"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(316..720)
                     /locus_tag="PKI05_08165"
     CDS             complement(316..720)
                     /locus_tag="PKI05_08165"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOA52218.1"
                     /translation="MYRVTREIDFCYGHRLLQYEGKCRYLHGHNGRLLVTFETDSLDK
                     LGMVLDFADIKRVLQGWIDENLDHRMILHREDPVVPVLQSMGEPLYLMAENPTAENIA
                     RHIFDYVRAAGLPVVETQLWETPRCSAIYRPA"
     gene            complement(802..1497)
                     /locus_tag="PKI05_08170"
     CDS             complement(802..1497)
                     /locus_tag="PKI05_08170"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HOA52219.1"
                     /translation="MRRESITNRDGVAVLLSGGLDSTITLMMVAEKSRSVFPLYIKCG
                     LTWENEELEAAKKICNWFDDKVAPLVQIDLPVAPIYGAHWSLTGQKVPSADSPDEAVF
                     LPGRNLLLLTVAGLWCRQHGVTRLAIGVLDSNPFADAAEGFFTKLEDLLNVYGPPEVR
                     IIRPLAGMSKTAIMHWERTLPLEETFSCIAPIDGRHCGRCNKCAERQRAFQEAGIPDR
                     TLYAFSKIEHGHS"
     gene            1777..2346
                     /locus_tag="PKI05_08175"
     CDS             1777..2346
                     /locus_tag="PKI05_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOA52220.1"
                     /translation="MVLSEIDRHLLERCLERKPRAWEDFVDRFLGLVIHVINHTARSR
                     SLRMTPELRDDLCSEVFLTLLKDDFAVLRRFRGQCSLASYLCVVVRRVVVKALLQRMG
                     DSHLQESSSVPPPKGLTTSSEEERINNREEAERLLSLLEGNEATVVRMFHLEGRSYQE
                     ISEATGIPTNSIGPILSRARSKLRRITTA"
     gene            complement(2414..>3314)
                     /locus_tag="PKI05_08180"
     CDS             complement(2414..>3314)
                     /locus_tag="PKI05_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52221.1"
                     /translation="SGMPYSGQSVSSSTYSAPQISPMPPTPPWVIAGTNDLASRPSGK
                     TTGTSGVAQSSKPGPLTIVNPTVVARTPTAPVDRPLPQNKLEISRSESIPDIAVAPWL
                     PSVATSNYTPLPGKTPSISTHVAQGNPSTATFATPEPGPAVTVWAEDSAESRVTPAGF
                     TVPSAKDATVANSPTAPVASVTANDVVPALNGYCPVELVENEAWVKGQARFAVEYQGK
                     TYFCGGATQKRKFQTNPERYVPVCNGRDPVLLVDQGVLSEGKIEHCAVYDGRLYMFSS
                     TASLARFRLNPQHYARTALQFQP"
BASE COUNT          749 a          914 c          892 g          759 t
ORIGIN      
        1 tttagcatga cgtgaacaac aaaaccagct tcaccgggaa ggacgctctt caacggcacc
       61 gagccacaat gtccaaagat gatccagcta gtaaggattt ctttagttac ccggcgtcca
      121 caggacggcg gtaagatagc ccaaatgggg tatcgagtga caaaacgtta tcccctcaag
      181 gatacagaat ccactaagtc gtgacgagat cggtttgacg cgtcggacac ctcttaaatg
      241 tgtacgagca tacatctata gcggggtaat gacgcgctct ttaggtgtct tcgaggggcc
      301 ccggcgtctc aaccatcatg ccgggcggta gatggctgaa catctgggtg tttcccacag
      361 ttgggtctcc acgacgggca agcccgcagc ccgcacgtag tcaaagatat gccgggcgat
      421 attctctgcg gtggggtttt cggccatcaa atagagtggc tcgcccatgg actgtagcac
      481 aggcacgacc gggtcctctc ggtgaaggat catccgatga tccagattct cgtcgatcca
      541 cccttggaga accctcttaa tatccgcgaa gtcgagaacc atccccagtt tgtcaagaga
      601 atccgtctca aaagttacaa gaagtcgccc attatgaccg tgcaggtaac ggcacttgcc
      661 ctcgtattgg agcaaccgat gaccataaca aaaatcaatc tcgcgtgtta cgcggtacat
      721 acttttccct ttggcgtgag tgcctccctg atttgcgcgt gtatgacgtt tatggtgcct
      781 aagcccagag aaaactctta actagctatg cccatgttca atcttgctaa atgcatacag
      841 tgtccgatcc ggtatcccgg cctcctgaaa tgcacgctgc cgctccgcgc atttattgca
      901 acgcccacaa tgtcggccgt caatcggcgc aatgcatgag aacgtctcct caagtggtaa
      961 tgtccgctcc cagtgcatga tggcggtctt gctcatgccg gccaacggcc gaatgatccg
     1021 cacttcaggc ggaccataaa cgtttaggag atcttccagc ttcgtgaaaa agccttccgc
     1081 tgcatcagcg aagggattgc tatcaagtac cccgatggcc aaacgagtca caccgtgctg
     1141 ccggcaccag agaccggcca cggtgaggag taggagattc cgtccgggca agaagaccgc
     1201 ttcatcaggc gaatcggcgc tgggaacctt ttgtccggtt aaactccaat gtgccccgta
     1261 gattggggcg accggcaggt caatctggac aagtggagca accttgtcgt caaaccagtt
     1321 gcaaattttc ttggccgcct caagctcctc attttcccaa gtgaggccac acttgatgta
     1381 cagagggaat accgacctgc tcttttccgc caccatcatc agagtgatag tgctatccaa
     1441 gccgccgctg aggaggacgg caacgccgtc gcgatttgtg attgattctc gtctcatgac
     1501 gtcagtactg gccagacttt ggaggctttt ttgtcccgca gccaatcttc aggcattata
     1561 agcccgttgc caaagcgaga ccgataatcg atagcgcgag accgcaaggc cgattaccac
     1621 tgttacccta tcatctccca cttgcggcat ttttctcttg cttacatctg tggggggcat
     1681 ttaaaatcca tataggatga gatggccacc cggcggggtg gacacttttg gtacatgaat
     1741 cggcgggcca ggtaaaaaca catcgggaga acaagcgtgg tcctctccga gattgatcgc
     1801 catctcctgg aacgctgtct cgaacgcaag ccgcgggcat gggaagactt tgtcgatcgt
     1861 tttctgggcc tcgtaatcca cgtcatcaat catacggccc gcagccgaag tctgcgaatg
     1921 acgcccgagc tccgcgacga cctctgctct gaagtctttt tgaccctcct gaaggacgat
     1981 tttgccgttc ttcgacgttt tcgagggcag tgcagccttg ccagttatct ttgcgtcgtt
     2041 gtccgaaggg ttgtcgtcaa ggcccttttg cagcggatgg gtgattcaca ccttcaagaa
     2101 tcctccagcg ttccaccgcc aaaaggattg acgacatcat cggaagaaga acgcatcaat
     2161 aaccgtgagg aagccgaaag actactcagc ctcctcgagg gaaatgaggc caccgtggtc
     2221 cgcatgttcc acctggaagg ccgaagctat caggaaatca gtgaggctac aggcattccg
     2281 accaacagca tcggccccat tttgagccgg gcccgcagca aactccgccg aatcaccacg
     2341 gcgtagcctc atcaagaata ccgatagtca aagcggccgt tgtacgatct ctccttgcga
     2401 tttcttactc ttcttatggc tggaactgaa gggcagtgcg agcgtaatgc tggggattta
     2461 gtcggaaacg ggcaagggac gcagtgcttg agaacatgta tagtcgccca tcgtaaaccg
     2521 cgcagtgctc gattttgcct tccgatagga caccttgatc cacgagtagg acagggtctc
     2581 ggccattgca tacggggacg tatcgttccg ggttcgtttg gaatttccgt ttttgtgtgg
     2641 ccccaccaca aaagtacgtc ttgccttgat attccaccgc aaaccgagcc tgccccttaa
     2701 cccatgcctc gttttccacg agctccaccg ggcaataacc gtttaacgcg gggaccacat
     2761 cattggccgt gactgaagcg accggtgctg tcggagaatt tgccactgtc gcatccttcg
     2821 cgcttgggac cgtgaagcct gcaggtgtga ccctactttc tgccgagtcc tcagcccaaa
     2881 cggtgacggc cggacctggt tcaggcgtcg caaaggtggc ggtacttggg tttccttggg
     2941 caacgtgcgt ggaaatgctg ggtgtttttc caggcagtgg cgtgtagttg cttgttgcca
     3001 cactgggaag ccacggcgct acggcaatgt ccggtatcga ctcgctacga gagatttcca
     3061 atttgttttg aggcaaagga cggtccaccg gggcagttgg ggtacgtgca accacggtgg
     3121 ggttaacgat ggtcaacggc cctggttttg acgactgggc aacaccgctc gtcccggttg
     3181 tctttcccga aggccgcgac gccaaatcat ttgttccggc aataacccaa ggtggtgtgg
     3241 gcggcattgg ggaaatttgt ggagcactgt aggttgagga actcacagat tggccggagt
     3301 acggcatgcc tgaa
//
LOCUS       DAOPTR010000381         3313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_22991_length_3313_cov_3.646102, whole genome shotgun sequence.
ACCESSION   DAOPTR010000381 DAOPTR010000000
VERSION     DAOPTR010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22991_length_3313_cov_3.646102"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..392
                     /locus_tag="PKI05_08185"
     CDS             180..392
                     /locus_tag="PKI05_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52222.1"
                     /translation="MEPVAIQGKSIRIVFPDISPRSWARYDAAEKCPAGHMLGGKKMW
                     NVADLRAWASLGFPDRATFERKRGDL"
     gene            389..829
                     /locus_tag="PKI05_08190"
     CDS             389..829
                     /locus_tag="PKI05_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52223.1"
                     /translation="MSYTPYLTCEAHGRQIATALPPDCPMKEQASYRALTSLLDEGFK
                     LIDPQTPETIAHGLWLVRRGQDTCYSCIGRLVKHPTIKHIRDDQQAKERYAATVGRPS
                     KSVEHLPPINDKAKARDQSPKPDWMKPNVAPVQPANKVEWRAPA"
     gene            811..1866
                     /locus_tag="PKI05_08195"
     CDS             811..1866
                     /locus_tag="PKI05_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOA52224.1"
                     /translation="MEGTGMRRPEPPAIIETTAIVYRLADIQERQQEWIWQGYIPKGC
                     FSIGYGDPNAGKTTAACHLSARITSGQDWPDGQPNPKPRSVLFLTGEDSVSRTLLPRF
                     RAAGGDVNKLVVWDRVRFNYEDGEKSERLPDLLADVGKLKDIITSCPDMALVVVDTLS
                     CFLAVKDSNQAQQVRAALLPLRDLCEQTGVTLLGLGHMNKSSGQKAQYRASGSISYIG
                     AARSALLFTPDRDDPETMVMAVSKCNLARRAPSLKYRIVPWNKDPEVAAVEWCGTSSY
                     SADQLLEEQKVNTKKKAIIEWLKTILKDGPLLSDTIMRMAAEEGFKERTVWNAKDDAG
                     VKARKQSFSGPWEWYIP"
     gene            2172..2372
                     /locus_tag="PKI05_08200"
     CDS             2172..2372
                     /locus_tag="PKI05_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52225.1"
                     /translation="MLLRQVRREVGLARGVAMNERVTITVEADTGRLPGVPLAVRVRR
                     LLKAMLRSFGLRAVEVREGHVR"
     gene            2603..3100
                     /locus_tag="PKI05_08205"
     CDS             2603..3100
                     /locus_tag="PKI05_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52226.1"
                     /translation="MQKPQRKTQKPGADFSLATGSQIAALFKVSPSAVSGWVRKHGCP
                     RRDDGLYDVAAVIAWKTERDTALVGGDSDALERWRAARAEAEELRVRRLKNELVEVQA
                     VRDEVTRLCTVVREHARELTDDIARELGVTGDQIKVLDDARRRYLDRCATDMESHYTK
                     GKVLI"
     gene            3100..3312
                     /locus_tag="PKI05_08210"
     CDS             3100..3312
                     /locus_tag="PKI05_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52227.1"
                     /translation="MARSIRQTMAVPPPVTAEVVASPRCPSCGSQAIRVVFSGGVLSC
                     DCMLCEKTFTDRPYHKEVKTDEQNHV"
BASE COUNT          745 a          989 c         1040 g          539 t
ORIGIN      
        1 ccgacattcg gcacgatctc ccaacgcgaa cacatccccg tccaccgctt gcggtacgcg
       61 gccgcgagac tgggcgtcaa gcccgtaggc aaagcgggcg gcacctacgt attcaccgag
      121 gcggacgcgg cggcaatcgt ccagaccgct cggaggattc agcgggggga gcaattctaa
      181 tggagcccgt cgccatccaa ggcaagtcca tacggatcgt attccccgac atcagtcccc
      241 gctcatgggc ccggtacgac gctgcggaga agtgccccgc cggccacatg ctaggcggaa
      301 agaagatgtg gaacgttgca gacctgaggg cgtgggccag cctgggcttt ccagaccgcg
      361 cgacgttcga gcgaaagcgg ggtgacttgt gagttacacc ccatacttga cctgcgaagc
      421 acacgggcga cagatcgcga ccgcgcttcc gcccgactgc ccgatgaaag aacaggcgtc
      481 ataccgcgcg ttgacgtccc tgctggacga ggggttcaag ctgatcgacc cgcagacccc
      541 ggagacgatc gcgcacggct tgtggctcgt gcggcgcggg caggatacgt gctattcgtg
      601 catcggccga ctcgttaagc atccgacgat aaagcacatt cgcgacgacc agcaggcgaa
      661 ggaacggtat gcggccaccg ttgggcgacc gtcgaaatcg gtggaacatt taccaccgat
      721 taacgacaag gccaaagctc gcgaccagtc cccgaagccc gattggatga agccaaacgt
      781 tgccccggtt cagccggcga acaaggttga atggagggca ccggcatgag gcgaccagaa
      841 ccgccggcca tcatcgaaac aacagccatc gtctatcgtc tcgcggacat tcaggagcgg
      901 cagcaagaat ggatctggca aggctacatt cccaagggct gcttcagcat cggctacggc
      961 gaccccaacg cgggcaaaac aacagcggct tgccatttat cggcacgcat cacgagcggg
     1021 caggactggc cagacggaca gcccaacccc aagcccagat cggtgctctt tttgaccggc
     1081 gaagacagcg taagcagaac actgcttccg aggttccgag cggcgggcgg cgacgtgaac
     1141 aagctcgtgg tatgggaccg cgtgcggttc aactacgagg acggagaaaa gagcgaacgt
     1201 ctgcccgatc tgctggccga cgtgggcaag ctcaaggaca tcattacgag ctgcccagat
     1261 atggccctgg ttgttgtgga cacgttgtcc tgcttcctgg ccgtcaaaga cagcaaccaa
     1321 gcccagcaag tgagagcggc actgctgccc ctacgtgacc tgtgcgagca aaccggggtt
     1381 acgctcctgg ggctcggcca catgaacaag tcctccggcc aaaaagccca atatagggcg
     1441 agcgggtcaa tcagttacat cggcgcggca cgctcagccc tgctgttcac acccgaccgc
     1501 gacgaccccg agacgatggt tatggccgtt tcgaagtgca acctggctcg gcgggcaccg
     1561 tccctgaagt atcgcatcgt gccatggaat aaagaccccg aggttgcggc cgtcgagtgg
     1621 tgcggcacat cgagctattc ggctgaccag ttgcttgagg agcaaaaggt caacaccaag
     1681 aaaaaagcga ttatcgaatg gctgaaaacc atcctgaagg acggcccgct actctcggac
     1741 accatcatga ggatggccgc cgaggaagga ttcaaggaaa gaaccgtctg gaatgccaag
     1801 gacgacgcgg gggtcaaggc gaggaaacag agcttcagcg gaccttggga atggtacata
     1861 ccgtaggctt acaaaacatt gcagccttgg gatattaata ggaatcttcg acatatagat
     1921 tgcagccttg ggggcacatt gcagccttcg aagatcacat ccgaaaacgc caaggctgcg
     1981 aaggctgcaa acatttcaaa ggcaaggtac atgaaacagc cgaccgccag atacgcgacc
     2041 gtctaccagt tgtcgagcta cacgcgggag cgggcggacc gcaccaaggc ggacctgaga
     2101 accgatcccc cgacgtgccc caaatgctcc aagcggttcg tgataatcca cctggacaat
     2161 gacggccgtc gatgctcctg cgtcaagtgc ggcgagaagt gggattggcc cgaggtgtcg
     2221 caatgaatga acgcgtgaca atcaccgtag aggccgacac gggccggctg cccggcgtgc
     2281 ccctggctgt ccgcgtgcga cggctactca aggcaatgct gcgatccttc gggctccgcg
     2341 ccgtcgaggt gagggaggga catgtgcgct agtaccagcc tgcggggctg cttacgtctc
     2401 cttgccccgc gaccactacc agccaggcgg atggggcttg gccgcgttcg ggtgcgacgg
     2461 tacgggaacg cgggggctgg tggaggttta cggcgggtcc ctctggaggg gcatacgtct
     2521 atcgcaggtc ggagcagccc gacgtgtccc gaaatttcac cccccatttt gtcagtttac
     2581 ttaaaacgtg gagcgcgaat ccatgcagaa accccaacga aagacgcaaa agccgggtgc
     2641 ggacttttca ctcgccaccg gctcgcaaat cgcagcgttg ttcaaggtga gcccatcggc
     2701 cgttagcggc tgggtacgca agcacggctg cccccgtcga gacgatgggc tgtatgacgt
     2761 ggcggcggtt atcgcctgga agaccgagcg cgataccgca ctggtcggcg gcgactcgga
     2821 cgccctggag cggtggaggg ctgcgagggc cgaggccgag gaattgcggg tgcggcggct
     2881 caaaaacgaa ctggtcgagg tgcaggccgt ccgcgacgaa gtgacccgcc tctgtaccgt
     2941 ggtccgcgag cacgcgcgag aactcaccga cgacatcgcc cgagagctcg gcgtgaccgg
     3001 cgaccaaatc aaggtgctcg acgatgcccg gcggcgttac ctggaccggt gcgccaccga
     3061 catggagagc cattacacga aagggaaggt gcttatctga tggcccgaag cattcgacag
     3121 acaatggccg tccccccgcc cgttacggcg gaagtggtcg cgagcccgcg ttgccccagt
     3181 tgcggaagcc aagctatccg cgttgtcttc tctggtggcg ttctctcgtg cgattgcatg
     3241 ttgtgcgaga agacgtttac cgaccgaccc taccacaaag aggtaaagac cgatgagcag
     3301 aaccatgttt agg
//
LOCUS       DAOPTR010000382         3310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_23023_length_3310_cov_3.100461, whole genome shotgun sequence.
ACCESSION   DAOPTR010000382 DAOPTR010000000
VERSION     DAOPTR010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23023_length_3310_cov_3.100461"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            723..2426
                     /locus_tag="PKI05_08215"
     CDS             723..2426
                     /locus_tag="PKI05_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HOA52228.1"
                     /translation="MLRIMALRQIYRHGTRWAEILSVLSKYGLADWIDRLGLEVARDL
                     IKGPTGEAIARLPWTTRIRLALTELGPTFIKLGQLLSTRPDVVGVALARELEELQEHV
                     TADPPEVVKEIISRELGRPLEEVFAEFHEEPLASASIGQVHRARLKTGEHVVIKVQHP
                     DIEGKLLADLEILSGLAFLAERIPDFQIYRPMSVVAELRRTLVREVDFVRELRNLQEF
                     RAHFEDSPDVRIPRAFPELSTKRLLVLEYLDGCKLTEQDRLKELGIDFDEVARRGAQA
                     YLKMIFEDGLYHADPHPGNIMVFPDETIGLLDFGMVGRLDDRLRERVQELLTALVSQD
                     SERLAIIVAEVGATPISLDRAALALDLADFVAYYGHMPIEQFDLAGALTEMIEILRRY
                     RIVLPARIALLLKTLIVLEGTAQLLSPRFHLLEVIRQYRRKVLWHVFSPRIALRRAQR
                     TYWELQSLLEVLPQALRDVVQQLRNGTFDIHLDHRGLEPSVNRLVVGLMTSALFLGSA
                     LMLAHRVPPLLSDLPLLGGWLSENMGSISAPGFVGISLSAIWGWRLWRAIRRSGRLDR
                     H"
     gene            complement(2516..2749)
                     /locus_tag="PKI05_08220"
     CDS             complement(2516..2749)
                     /locus_tag="PKI05_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52229.1"
                     /translation="MASKALSGVSDQMAQHLEIDEQLALIRDLESRHEDLFRKLEELE
                     HRVAEVLTQWAKPLSRGDASSSLAEQVAPQSKN"
BASE COUNT          675 a          883 c          946 g          806 t
ORIGIN      
        1 cccgcaactg tctttacggt acgtcgtcga tctccaagct accgacgctc gcctcggccc
       61 gcatgacaag taaacttgcc accgggcctt gcctcgtgcg ctcggcacaa agatgccacc
      121 aaagatcaca accaaattgt caaagaacaa aacacggagc gacgactact cacttcgcca
      181 tcactccgac gcaaaccaac tggggtcaca tttccgaaaa catgtcccct gtttgtttgc
      241 gtcttacgtg tttgccaccc ctctaagttc aatttatggg cagtccacac accctgtcaa
      301 tcccatccct ttcgcagttt tttaggtcgc ttgtcgtcca gggccaattt ccattctatc
      361 ggtcgccccg ccccaggcaa gcccgcccga tttgattttc ttgggggctt gtcacttctg
      421 cctgccgcca tttcgcactg cccattttca tttgatccat cggcggacag ggcgccaagg
      481 ggggacatcg acccattttt gccccccggt cgttcaggtc atccgtccac ctgattggtc
      541 gataaagacc attcctggcc cgctttccct aaaatatcgt cccaatcggt cagaaagtcc
      601 agtccgggcg gtgccgaaag ctcccaatct gtgttcccat gctacgcggc gcggtatgct
      661 ttttgcagga aagaagtgcg cggaactgtg tcacttggcc tccttgagca gttaggccga
      721 atatgcttcg gatcatggcg ctgcggcaga tctaccggca cgggacccgt tgggccgaaa
      781 tcctttccgt gctaagcaaa tacgggctag cagattggat tgaccggctc ggcttggagg
      841 ttgcgcgtga cctcatcaag ggccctacgg gggaggcgat cgcgcgtctc ccttggacga
      901 cacgcatccg cctggcccta acggaacttg ggccaacatt tatcaagctt ggccagctct
      961 tgagtacccg tcctgatgtg gtcggtgtgg ccctcgcccg tgagttggaa gaactgcaag
     1021 agcatgtcac ggctgatccg cctgaggtag tgaaggagat catcagccgt gagctcggcc
     1081 gtcctttgga agaggttttt gctgaatttc acgaagaacc attggcctcg gcctcgatcg
     1141 gacaggtgca tcgggcgaga ttgaaaaccg gtgagcacgt ggtaatcaag gtccagcatc
     1201 ccgacatcga aggaaaacta ttggccgatc tggaaatcct cagcggttta gcgtttctgg
     1261 ctgagagaat tcccgatttc cagatctatc ggccaatgtc ggtcgtcgca gagttgcgaa
     1321 ggacgctggt ccgggaggtg gatttcgttc gggaacttcg caatctgcag gaatttcggg
     1381 ctcattttga ggattcgccg gacgtccgta tcccgcgggc attcccggag ttgagcacga
     1441 aaaggcttct tgtcctggag taccttgatg gttgcaagtt gacagagcag gaccggctta
     1501 aagaattggg cattgacttt gacgaagttg cccgccgtgg ggcacaagcg tatctcaaga
     1561 tgattttcga agatggcctc tatcacgccg atccccatcc tggaaacatt atggtctttc
     1621 ccgatgaaac gatcggtctt ttggattttg ggatggtagg gcgactggac gatcgtctcc
     1681 gggagcgggt gcaagagttg ctcaccgccc ttgtcagtca ggacagtgaa cgtttggcga
     1741 tcatcgtggc agaggtcggg gccacgccga tttcgctgga ccgggcggct ttggcacttg
     1801 atctggccga cttcgtggcc tattacggac acatgccaat cgagcaattc gaccttgccg
     1861 gcgctttgac ggagatgatc gaaatcctcc gtcgttatcg gattgtgtta ccggcgagga
     1921 ttgcccttct cctaaagact ctcatcgtgt tggaggggac ggcacaactg ctaagcccca
     1981 ggtttcatct cttggaggtc atccgccagt atcgccgcaa ggtcctttgg catgtgttct
     2041 caccacgaat cgcccttcgt cgagcgcagc gaacctattg ggaattgcag agccttcttg
     2101 aggtcctccc ccaggcctta cgagacgtcg tccaacaatt gcggaatgga acgtttgaca
     2161 ttcatcttga ccatcgcggt ttagaaccat ccgtcaatcg ccttgtggtg ggactaatga
     2221 cgagtgccct gtttctcggc tccgctttga tgctggccca tcgagttccg ccgcttctga
     2281 gcgatttgcc gctgctcggc ggatggttaa gcgagaatat gggatcgatt tcggcgcccg
     2341 gcttcgtcgg tatcagcttg agtgccatct ggggatggcg gttatggcgg gcgatcaggc
     2401 gatcgggaag gctcgatcga cattgacggc gacgctaggt ccagctttcc tgcaggtgat
     2461 accaggcgtg cgaaaaaccg agggataatc ttcggcaagt cgcccggggc cggtcttaat
     2521 tcttgctttg cggagccact tgctccgcta atgaactgga cgcatcgccc ctggaaagcg
     2581 gtttagccca ttgcgtgagg acctccgcca cacgatgttc gagttcttcc agttttcgaa
     2641 agaggtcttc gtggcgactt tcaagatcgc ggatcagggc aagctgctca tcaatttcga
     2701 ggtgctgcgc catttggtca gaaacgccgc tgagagcttt ggaagccata tcttcggttg
     2761 ctcgtgcgta gccaggaaaa cggcaaagga atcaacgctg tcggttacgc tttgtcgccg
     2821 tgaggttggg aggactggga ataagtttcg gacagcacat ggccgaacca tttgggtaaa
     2881 gccagatttt cgtcctgcga cgattgcgaa ggaggaataa tatagcaaag atgggaagga
     2941 tgtgcggata gtcccgtgtc cgattttggg gggaccgttt ttcgtcagcc cgaggtatct
     3001 ccctttagaa tatccccccg attagcaatg cgcacccgct ggacaggagg ataaaaagcc
     3061 gttacgatca gactttgccg atgagactcg aggaggccgt tggcttggcc ggagttgttt
     3121 ggctcatggc cggtctggac tgcggttgcc gagtatttcc cgcgaagtga ctttcccgaa
     3181 agttttgcca ggtcaagaag cggggactag tcggtcgatc cataaaattt attaggggca
     3241 atccctagtg gggcaggcga gaactgttgt agcgaagggt ttttaagtag gtttgggttt
     3301 ttgtcgtttg
//
LOCUS       DAOPTR010000383         3308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_23041_length_3308_cov_4.110667, whole genome shotgun sequence.
ACCESSION   DAOPTR010000383 DAOPTR010000000
VERSION     DAOPTR010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23041_length_3308_cov_4.110667"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="PKI05_08225"
     CDS             complement(<1..865)
                     /locus_tag="PKI05_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOA52230.1"
                     /translation="MATTSVIYVISDDPRLGEQVEQAVRDLFSVERRASFDALERLGP
                     GYCTVCPAVVAHLTKKTVNGFPAKELLQKLGQLERIAPVITIGDENLTKEVTGQANGA
                     GRVNFPAAFRPEQLRQAVAELSPVEWELAELFHYRPHAVLRGGHRQWVTFTPSVFEMV
                     EELRVAAAHDVSLLLIGETGAGKTFLARTIHEISPRAGKRFLTVACGALPPDLIESEL
                     FGYVKGAFTGADTDKPGKFAAVDDGTLLLDEIDVLAPEHQAKLLRVIETGEYEPVGSN
                     ETAFSRARLIVA"
     gene            1220..1651
                     /locus_tag="PKI05_08230"
     CDS             1220..1651
                     /locus_tag="PKI05_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52231.1"
                     /translation="MTSLMLSASLLIAMAGAPGDVMPVTQEVVVAEEVPVSGHIAATG
                     HRPTATGHGWLVDWFGPMPQTCYQPRFGCYPGNPRTIHRYPAFHGYYYRAPYNYRHLF
                     EYPWHAQPYESEPLRAQSAEVLIDPTASTSTLQPVPEQALQ"
     gene            2061..2615
                     /locus_tag="PKI05_08235"
     CDS             2061..2615
                     /locus_tag="PKI05_08235"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOA52232.1"
                     /translation="MNSLQGYLLVASPYLADPNFVGSVVLIIQHTENGAFGVILNRIH
                     PKKIKDLWKEVSESPCRYDRHLNIGGPVSGPLMALHDDPSLAELEVIPGVYFSVQRDN
                     IEQLMAAQGHEARVFLGHSGWAGGQLEGELAQGSWLLAPATKEIVFSDDERHLWQKVA
                     FAVGRAILADAIRVKHRPEFPWLN"
     gene            complement(2801..3115)
                     /locus_tag="PKI05_08240"
     CDS             complement(2801..3115)
                     /locus_tag="PKI05_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52233.1"
                     /translation="MTSSHDPDKPSSSRGWPQVLGRLFLYARSVGSNLHTHAEAIGPL
                     ANGMGLALVIGGAIWLLASRHPLALAMVFLGTALYLATRFPGGPEKPSPPAGKQPKPR
                     QG"
BASE COUNT          717 a          996 c          917 g          678 t
ORIGIN      
        1 aagccacaat caggcgcgcc cgcgagaacg ccgtctcgtt gcttcccaca ggctcgtatt
       61 ccccggtctc gatcacccgg agtaacttgg cctggtgttc cggggcaagg acgtcgattt
      121 cgtccaagag taacgtgccg tcatccacgg cagcaaactt gcctggctta tccgtatccg
      181 ccccggtgaa cgcccctttg acgtagccaa acagttcgct ctcgatcagg tccggtggca
      241 gggcaccaca ggctaccgtg agaaagcgtt ttcctgcccg cggcgaaatc tcgtgaatcg
      301 ttcttgccaa aaaggtcttt cccgctcctg tttccccaat cagcagcagg ctgacgtcat
      361 gtgccgccgc tacgcgaagt tcttccacca tctcgaagac ggaaggcgtg aaggtcaccc
      421 attggcgatg ccctccccgc aggacagcat ggggacggta atgaaagagt tcggccagtt
      481 cccattccac gggcgagagt tccgccaccg cctggcgaag ctgttctgga cgaaaggccg
      541 ccggaaaatt cacccggcca gccccgttag cctgcccggt cacttccttc gtgagattct
      601 cgtcgccaat ggtgataaca ggagcaatcc gctcaagttg ccccagcttc tggagcagct
      661 ctttggccgg gaaaccattc accgtcttct tcgtgaggtg agcaacaacg gccgggcaaa
      721 cggtgcaata accgggtccc aaacgctcca gcgcatcaaa cgaagctcgt cgctctacgg
      781 agaacaagtc gcgaacagcc tgctcgacct gttcgccgag acgcgggtcg tcgctgatga
      841 cgtaaataac agaggtcgtt gccatactca tctcacacgg cctcgataat tcacgataca
      901 cttttgcgga aaagaacctt ggacgagttg acttccgaaa acagtggcca attcgcaagg
      961 aaaggctgtt ctgatgtgac ccacatcacg tttacccaga ttatctatgt tgcctatact
     1021 agttaaggaa ccgaaatact caagtcgggt ccgccagtgt tccgaggaaa atcagctctt
     1081 ccgcctgtcc gtgcggtccg gtcgtgcaga gtttagcgat caagactgga tttatcagct
     1141 ttgctgggca accgcgccga tagcaaaaaa gaggaaccct atctcttggc ccatcaagtt
     1201 ttattgtgag gaccaaatca tgacgtccct gatgctgtcg gcatcactct taatcgccat
     1261 ggcaggagca cccggcgatg ttatgccggt tacccaagaa gttgtggtcg cggaagaagt
     1321 tcccgtctca gggcacatcg ctgcgacggg ccacaggccg acagccacgg ggcatggctg
     1381 gctcgtcgac tggtttgggc ccatgccgca aacctgttac cagcctagat tcggatgcta
     1441 ccccggtaat ccgcggacga tccaccgtta tcccgccttc cacggctact actaccgggc
     1501 gccgtacaac tatcgacacc tcttcgagta cccctggcac gcccaaccct acgagtccga
     1561 gccattgcgt gcccaaagtg ccgaggtctt gattgacccg actgcaagca catccacgct
     1621 ccaacccgtg ccggaacaag ccctacaata gagtccacca acccggccac gacgagccgc
     1681 ttagcactca caacgggctt tcggccagtg taaaccaccg ctacggccgt tcggctagtt
     1741 acgctatatc ctctgtagcc atccgcgaaa ttcatggctt tccggcgtga ctgcgaaaac
     1801 cgccgtcacg aggtcggcca gcgcgggcga ctatcccccc tgaaggctgg gacgcttttc
     1861 cttcaacgat gcttctcggt ccccccgggt cggtccatta tcgcgcccac cggtaggaac
     1921 ccctctttac gcggtcactc tggcatccaa gaataggaag ccccaaatgc ttggtcgtgt
     1981 cgtgcgtata tattactagt ggaggtagtt tggcttggaa gcacagggta acgggaacac
     2041 ctaacgggag gacgacaaag atgaactcgc tgcaaggata tctgctcgtg gcgtccccct
     2101 atttggcgga tccgaacttc gtcggttcgg tggtgttgat tatccagcac accgaaaacg
     2161 gggcgtttgg cgttattttg aaccggattc atcccaaaaa gattaaggat ctatggaaag
     2221 aggtgtccga aagcccctgc cggtacgatc ggcatttgaa catcggtggc cccgtcagtg
     2281 gccccttgat ggccctgcac gatgatccct ccctggcaga actagaggtc atccctgggg
     2341 tatatttcag cgttcaacgg gacaatattg agcaactcat ggcggctcag ggccatgagg
     2401 cccgagtgtt cttgggccac tcgggctggg caggaggaca actcgaaggc gaactggcgc
     2461 aggggtcttg gttgttggcc ccggccacca aggaaatcgt tttctcggac gacgaacggc
     2521 atctctggca aaaggtcgcc ttcgctgtgg ggcgggccat tctggcggat gcgatccgcg
     2581 tcaagcaccg tccggaattc ccgtggttga actgaaaaag ccttcccagg cggcttgtcg
     2641 cccactggcc gcagcgtctt ttcttgggag accttgcact agtgctccgc cttaaaacat
     2701 gtcggtgacg ctagcaaaag agtgaaggcc cagatcaaag ccgcaacggc gcggcttctc
     2761 agaatgattc ccacggccgt ttgcgcgacg ccatcctggt ttatccctgt ctcggctttg
     2821 gctgtttacc ggcaggaggg gacggcttct ccggtccacc gggaaaacgg gtggccagat
     2881 ataaggcggt tcccaagaaa accatagcca gggccagcgg atgacggctc gccaaaagcc
     2941 agattgcacc accaatgact agcgccaatc ccatcccgtt ggccagtggg ccaatagcct
     3001 cggcgtgtgt gtgcaaattt gagccgacgc ttcgggcata cagaaagagc cgtcccaaga
     3061 cttggggcca accacgggat gacgacggct tgtcagggtc gtgtgagctt gtcatcgaac
     3121 atcgccgaac gctcggcgct tagagcctat ccccaaaaag accccctcgg cctccgggag
     3181 agggttgggg tgagggcaag cgaacgtcgg agttgatcga gaaaaccggc agcgattcgc
     3241 gcaatcctct ccacggccct tctcccgccg aacgcgtggg gggagattat gggataggct
     3301 cttagtcg
//
LOCUS       DAOPTR010000384         3307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_23051_length_3307_cov_2.444649, whole genome shotgun sequence.
ACCESSION   DAOPTR010000384 DAOPTR010000000
VERSION     DAOPTR010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23051_length_3307_cov_2.444649"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PKI05_08245"
     CDS             complement(<1..202)
                     /locus_tag="PKI05_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008695132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HOA52234.1"
                     /translation="MPGLPQSSSVQERRQQLLELIRTRRFATLEELAAALGVSESTVR
                     RDLEALEQQGDARRIHGGALYTG"
     gene            631..2193
                     /locus_tag="PKI05_08250"
     CDS             631..2193
                     /locus_tag="PKI05_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52235.1"
                     /translation="MNRPQPSSSAQRPQPTLRVLGRLPYVAPSSEDSAGVSESEPSLN
                     HTPLALSEPVRRGLIYGVATLLVLGGLWYIVSRKTDNTELASKSPDKSWEVQMPRPDA
                     PEAPAWSPHNSGEMQAQSAQAQESSVPVGSFAGPQTPAKSPLGFDLPPGNSWGQPTAT
                     ENAFAQPAETAGVPYSPQTANAGAPYSAFEAQTAFNGWNGGASNGFANPQSMSAFPQG
                     VVTTPPWVNAAGQPSAGQPGGFTAERLPLNQGETALQPTSPSGPVVMRNPYVVSGNTP
                     GATLPSGLDQAGYTATSTPTGMATGPYGTPSLGPAAPTPNAWSASGYPTMPYEAVASR
                     PRNDWQTPAVSNVQQPTSGYPANTQPAMSGWNQAGYGVTSLVPAAAIQDASASSAGYP
                     SATYPQSDGSMSTTQQLPGWKPSPPVPSFPSWNVPPSHATPSPSYPGSTNPYPPANRP
                     NPQPDWGQDPGVVPATYANSVNADSRLPQWSYPSTQNPSATSVPNTNTTSPQYPSTGQ
                     TGYGLYPSTTLR"
     gene            2294..2902
                     /locus_tag="PKI05_08255"
     CDS             2294..2902
                     /locus_tag="PKI05_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52236.1"
                     /translation="MMSQQTPSAVRLQDAFLRLQDKPHGLVVKEPATTGPSANAHDTE
                     ETCAEAATSFRPALEVDQFAPPPLVSQLLESISPILAGCLRQIRQRLPKSERVVGLIS
                     PERGHGCTTTAILLASRLSRERATALVIDASPDRALADSLSLEIESGWEQAMLRRRPL
                     REVIIRSNSDGFDILPGQPGSLASKVTGVQPTDWPELLKAYD"
     gene            3011..3193
                     /locus_tag="PKI05_08260"
     CDS             3011..3193
                     /locus_tag="PKI05_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52237.1"
                     /translation="MGTSVPFVSLCSSFVMVQRCDSGTARFSLPAGAAEILGVIETFV
                     QPTAESQVLVNPKEAA"
BASE COUNT          702 a         1045 c          885 g          675 t
ORIGIN      
        1 ttcccgtata gagggcaccg ccatgaatcc ttcgcgcatc accctgctgt tctagggcct
       61 ccaagtcacg ccgcactgtc gattcggaga caccgagggc tgccgccaac tcctcgagcg
      121 tggcaaaccg ccgcgtgcga attagctcga gcagttgctg tcgccgctct tgaacgctcg
      181 aggattgggg caaaccgggc ataattggca aaaatctacc agtattatgc aaaaattatt
      241 gctaagtagt gaagatcgga ctaaggtatc tacagattat aggatagcct tctgtcgcat
      301 atccgtcaag agagtgacgc ctatttggcg tcttttccga cgatgacgcc aggacgtggc
      361 ccaaacctgc gccgcagtga gatagatatc tgtcggtctc tgacccgatt ttagcatcga
      421 cgctgttgcg ttgcgtcatc gtacgccccg agatttgcca aaaccggcat atccggaaaa
      481 accctcaagg tttaccgaac ctcgtccgat tctaacgggc agtggccggg gagtcgcctt
      541 tcggcgatcc cggtgatcgt atggtcagtg ccctacgcaa aaagctgaaa ctttcaacga
      601 cgttttacgt cccaaattcg gagaactgtc atgaaccgac ctcagccctc ttcttcggca
      661 caacgaccgc agcccacgtt gcgggttctc ggtcggttgc cctacgtggc cccttcctct
      721 gaggattccg ccggtgtctc ggaaagcgag ccctcgctca accatacgcc tcttgccctc
      781 agtgagccgg tccggcgggg ccttatttac ggtgtcgcca ccctgctcgt attgggcggg
      841 ctctggtaca tcgtgagccg aaagacggac aacaccgagc ttgccagcaa aagccctgac
      901 aaatcctggg aggtgcagat gccgcgacca gatgcaccgg aagcacccgc gtggagtccc
      961 cataactcag gcgaaatgca agcccagtct gctcaggccc aggagtcctc ggttcccgtc
     1021 gggtcctttg ccggcccgca aactccggca aagtctcccc tcggcttcga tctcccaccc
     1081 ggcaatagtt ggggccaacc gacggcaaca gaaaatgcat tcgcccaacc cgcggagacg
     1141 gcgggcgtcc cctatagtcc tcaaaccgcg aatgccggcg ccccctactc cgcgttcgaa
     1201 gcccagacag catttaatgg atggaacgga ggagcgagca atggctttgc caatcctcag
     1261 tccatgtccg ctttccctca gggagtggtc accactccgc cttgggtcaa cgcggcgggc
     1321 caaccgtccg caggccaacc aggcggtttt acggccgaac gccttcccct gaatcagggt
     1381 gagaccgcac tacagcccac aagtcccagt ggtccggttg tcatgagaaa tccttacgtc
     1441 gttagtggaa acacccctgg ggcaactctt cccagcggac tggaccaggc cggttacacc
     1501 gcaacaagca cgcccactgg aatggccacc ggaccctatg ggacgccttc cctaggtccc
     1561 gcagcgccga cgccgaatgc ctggtccgcc tcgggatacc caacgatgcc ttacgaggcc
     1621 gtcgcctcgc gaccgagaaa tgactggcaa acccccgccg tgtcgaacgt tcagcaaccc
     1681 acctccggat atcctgccaa cacccagccc gctatgagtg gttggaacca agccggttac
     1741 ggagtcacga gcctcgttcc cgctgcggcg attcaggatg cctcggcttc cagcgcaggg
     1801 tatccgtcgg ccacttatcc gcaatcggat ggaagtatgt caacgacgca acagttacca
     1861 ggatggaaac cgtcacctcc agtgccaagt ttcccttctt ggaacgttcc gccaagccac
     1921 gcaacgccat cccccagtta cccggggtca accaacccct atcctccggc gaatcggcca
     1981 aatccgcagc ccgactgggg ccaggatcct ggcgtggtcc ctgccacgta tgccaactcg
     2041 gtaaatgcgg acagccgttt accgcaatgg tcgtaccctt cgacacagaa cccctcggcg
     2101 acttctgtac cgaacaccaa caccacgtca ccccagtatc cttcgacagg ccaaactggg
     2161 tatggcctat acccctcaac gacacttcgt tgattgggga tttggatgat tcgttccgag
     2221 gatgtcgcca tcgtggcatt tccgcggatg tccaccggga acgtacttgg ggtccttggg
     2281 aggtaggaat cacatgatga gccaacaaac gccgtctgct gtgcgccttc aagacgcctt
     2341 tctccgcttg caagacaagc cacacgggct cgttgttaag gagccggcca ccactgggcc
     2401 atcggccaat gctcatgaca ccgaggaaac ttgtgccgag gcagctacga gctttcgccc
     2461 cgcattagag gttgaccaat tcgctccccc tccgttggtc agccaactct tggagagcat
     2521 ttcgcccata ctggcaggtt gcctccgtca gatacgccaa agacttccta aatccgagcg
     2581 cgtcgtgggc cttatttcac ctgaaagagg ccatggctgc acaactacag cgatcctgct
     2641 cgcgtctcga ttatcaaggg aacgagcaac tgctctggtg attgacgcaa gcccggaccg
     2701 ggcgctggcc gactccctat cgctggagat agagagcggc tgggagcagg ccatgctgcg
     2761 tcggcgcccc ctgagagaag tgatcatccg ttccaatagt gacggctttg atattttgcc
     2821 aggccaaccg ggatcgctgg cttcaaaggt tacgggcgtc caacccaccg attggccaga
     2881 gctcttaaag gcctacgatt gaatcctcat cgattttggt tcgtcttttg cccgaaccgc
     2941 gtattcaccc accggaacta atctgtcaga gaatgatggc gaagaaagcg actcggtggt
     3001 gcgttcgggg atgggaactt cggtaccatt tgtctccctt tgctcatcct ttgtcatggt
     3061 acaaaggtgc gacagcggga ccgcgcggtt ttcgctcccg gccggggctg ccgagattct
     3121 cggtgtgatt gaaacctttg tccagccgac ggccgagtct caagtcttgg tcaatcccaa
     3181 agaggccgcg taaacgggtg tcagcgagca gaacagaccg cctgaaggtc gaaccatgta
     3241 cgggaactac tggcattttc gtgaaccacc gtttcggaat cagttccggc gggcattctt
     3301 ttttgcg
//
LOCUS       DAOPTR010000385         3304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_23082_length_3304_cov_3.620806, whole genome shotgun sequence.
ACCESSION   DAOPTR010000385 DAOPTR010000000
VERSION     DAOPTR010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23082_length_3304_cov_3.620806"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PKI05_08265"
     CDS             <1..451
                     /locus_tag="PKI05_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009095758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetylesterase"
                     /protein_id="HOA52238.1"
                     /translation="RNEDGRARGAIASVYQNLSYEEVKPLLPEIYRAVVELAPSDEMF
                     GDGIRLAGLKVLAQHRIREGMSLCLDIMELDRWGKQDRVMGGLEALSLYGAAAKPLLP
                     RLRQLEKELLAHWEARSFQPAVERLRALIAKIENSNETVTLRSLQDP"
     gene            801..2606
                     /locus_tag="PKI05_08270"
     CDS             801..2606
                     /locus_tag="PKI05_08270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOA52239.1"
                     /translation="MLESVATGGASSAKEEKYSLLLEVAFIIFAVVLSLLGIESLPII
                     GEEPRRAEVAREMLATNDWLIPREQGEIYCSRPPLQNWLIAVIAIIRGGFDAVTVRLP
                     SALATCATALVCFAFARHHLGRMAAITSGVAFLTMGQTLTIGQMGETDALFAFFVGSA
                     LLIWYHGYATPDKRTLFWALAGLLTGLGALTKALQAPVYYLMCTVTFLLIEKNYRLIL
                     SRAYVLSYIIILVVIAAWTVPYYLAVGPQYTWQIWCGQVESRLATEGLFGHILKYPLE
                     TFVCMLPWSLFFLAFLDHRLRKSLTNWQPLVRYLVVSLLITFPTVWFIPEAKNRYFLP
                     LYPLAAVLASVPWQYWVTGCTSERVIVPRLVWRLLQAFSAMILIALLAVWPIWLGAVK
                     IELITQSTLSLDKLFIVTMLATLMVIFIHAAIRINDSRSWMCVLLSLGITLGHGHRLI
                     IVPIQCGILYSPEPAIKQLAWDVPNPERLVSIGPIVARFRYFYPHLVKLVKEEEITQG
                     PPSGVDHFCLEGEWVRRARPRKKETDRPRMAIAYRGGRVIVPKLPFAWDVVAIIPLGR
                     TLTRDPQPVAVIGRIRQGGALLSSPLRVEDGKEIE"
     gene            2947..3282
                     /locus_tag="PKI05_08275"
     CDS             2947..3282
                     /locus_tag="PKI05_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52240.1"
                     /translation="MTLNYAQNMMVATRTIRWRPNWWGWGTLAVLALAFGVGLPALHN
                     YQSQRIVERIRARAEAAEKRLAAAKAAGHEEEIGKALTDLVKNYDLYLRHRPGETAAQ
                     IALALAAGQ"
BASE COUNT          691 a          859 c          953 g          801 t
ORIGIN      
        1 tcgcaatgaa gatggtcggg cccgaggtgc gatcgcctcc gtttaccaga atctctctta
       61 cgaggaagtg aagcctctct tgcccgagat ttatcgcgct gttgtcgagc tagcaccaag
      121 cgatgaaatg ttcggtgatg gaattcgctt ggccgggctg aaagtgcttg ctcaacaccg
      181 tatccgcgag ggcatgtcgc tttgtcttga tatcatggaa cttgatcgct ggggaaaaca
      241 agatcgagtc atggggggcc tagaagctct cagcctctat ggggcggcag ccaagccgct
      301 gctaccgcga ttgcgccaac tcgagaaaga actgctggct cactgggagg ctcgcagttt
      361 tcagccggcc gtggaacgac tccgagcgct tatcgcgaaa atcgaaaaca gcaacgagac
      421 tgtcaccctg cgtagcctgc aagacccgtg agctaactcc gaaccgttgc cgttttcgcg
      481 ggactttcac aagtctttcg ccgagaaatc cggcaggtag tatgctgtcg atcaaaggat
      541 aagagagtgc taggcggtcc ttttacccgc cattccttgt ccgaaacggt gcacgcgccg
      601 tgagcggatt cgagcgaaat gtcttcctct gccggctcac ggcccccgcg ccggctgtac
      661 cctccaagaa cggcccaatc ggtgtttaag cgaaaaaggg gccaacgata taattcgaga
      721 cgcggggcgg tcaggcgctg cccggtgaaa gaaggaaaag ggaggttcat gatcatctcg
      781 gcgagaggtt ggtcagccgt atgttggaaa gtgttgccac gggaggagct tcttcggcga
      841 aggaagaaaa gtattccctc ctcctcgaag tagcctttat catcttcgcg gttgttcttt
      901 cgttattagg gatcgagtct cttcccatca taggtgaaga gcctcggcgg gccgaggtcg
      961 cgcgcgaaat gctggcgaca aatgactggc tgatccctcg cgaacaaggg gagatttatt
     1021 gctctcggcc accgcttcag aattggctta ttgcggtcat cgccatcatt cgcgggggat
     1081 tcgacgcggt caccgtgcga ctgccgagcg cgcttgcgac atgtgcgacc gccttggtgt
     1141 gcttcgcctt tgcccgacat catcttggcc gaatggccgc tataacgtcg ggtgtggctt
     1201 ttctgacgat gggtcagaca ctgacgattg gccaaatggg cgagaccgat gccctctttg
     1261 cttttttcgt tggctccgcg cttttgatct ggtaccacgg ctacgccaca cctgacaaac
     1321 ggacgttgtt ctgggctttg gctgggttgc ttacgggcct aggtgcgctg acaaaggccc
     1381 ttcaagctcc agtatattac ttaatgtgta ctgtcacgtt tcttctgatc gagaagaact
     1441 atcgactcat tttatcgcgg gcgtatgttc tgtcctatat aatcattttg gtagttattg
     1501 ccgcctggac agtcccctat tatcttgcgg tgggaccaca atatacctgg caaatttggt
     1561 gtggtcaggt agaaagccga cttgccacgg aggggctctt cggccacatc ctgaaatatc
     1621 ccttagaaac attcgtgtgc atgttgccgt ggtcgctatt ctttctggcg ttcttggatc
     1681 acagattacg gaagtccttg accaactggc agcccttggt tcgttacctg gttgtcagtt
     1741 tgctcataac ctttcccacg gtttggttca ttccagaggc gaagaaccgc tattttctgc
     1801 cgctttatcc cttggccgca gtgcttgctt cagttccgtg gcaatactgg gtgacgggat
     1861 gcacatccga gagggtaatc gtaccgcggc tagtctggcg attactccaa gcgttctcag
     1921 ccatgatcct gattgcgctt ctggcagtgt ggccgatttg gctcggcgcc gtaaagatcg
     1981 aattaatcac ccaaagtacc cttagccttg ataaactgtt tatagtcaca atgctggcga
     2041 ctttaatggt aatatttatt catgcagcta tacggataaa tgatagccgg tcgtggatgt
     2101 gtgttctatt gtctttgggt atcacgctcg gtcacggaca tcgtcttatc atcgtaccga
     2161 tccaatgtgg aatcttatat tcgccggagc ctgctatcaa gcaactcgcg tgggatgttc
     2221 ccaatccaga gcgcctggtc agtattggtc ccattgtggc ccgatttcgt tacttttatc
     2281 cccatctggt gaagctcgtg aaagaggaag aaatcactca gggtccacca tcaggggtgg
     2341 atcatttttg tttggagggt gagtgggtcc gcagagcacg gccgagaaag aaggagacgg
     2401 accggccccg gatggcgatt gcataccgcg gtggccgcgt gattgttccg aaactcccgt
     2461 tcgcctggga cgtggtagcc atcatcccct tggggcggac gttgacacgt gacccacaac
     2521 cggtggccgt cattggcagg attcggcagg ggggagcgct gttgtcgtct ccactgcggg
     2581 tcgaggacgg caaggagatt gagtagtccc aatcagagca aacattgcac tggttcggga
     2641 cggccattgc caaaacgcga ccgagctctg gcattagaag ccggttttcc cgcaaggaac
     2701 tcgggtctaa caaaccactt atcacttttt ccagggttgc cgccggtcta tactcatggt
     2761 acgcgctggg ggcgctcata tttcccatgg aaatcccatg gaaaaaaggt ttcccgttgt
     2821 ccccgcgagg tgatctgcaa agcttgcggg acttgcgtgc ctgtgaggaa agagggtgtc
     2881 ttggtattgt gggtaaaatg agaactatgg agatatggcc aagtttagga aaatggttag
     2941 agtaaagtga ctctaaacta tgcccaaaac atgatggtgg caacgcgaac aatccggtgg
     3001 cggccgaact ggtggggctg ggggaccctt gcggtcctcg cgctggcgtt tggcgtgggc
     3061 ttgccggccc tccacaacta tcagtcgcag cggatcgtcg agcggattcg cgcccgtgcc
     3121 gaggcagccg aaaaacggtt agctgcggcc aaggcggctg gtcacgagga ggaaatcggc
     3181 aaggccctca ccgatcttgt taagaactac gacctgtacc ttcggcatcg acccggcgaa
     3241 acagcagctc aaatcgcctt ggccttggcc gccggccagt aagcggtctg ccgcgcgcgg
     3301 atca
//
LOCUS       DAOPTR010000386         3298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_23130_length_3298_cov_3.787851, whole genome shotgun sequence.
ACCESSION   DAOPTR010000386 DAOPTR010000000
VERSION     DAOPTR010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23130_length_3298_cov_3.787851"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="PKI05_08280"
     CDS             <1..984
                     /locus_tag="PKI05_08280"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOA52241.1"
                     /translation="IGYHLIKALSEGDHEIRCLARKTSRIEPLRPFGVQFVQGDIRDA
                     DSITGALSDVQIVFHLAGTTRALRREEFFRVNEHGTRHLLDACLRCKAPPVVVFVSSL
                     AAAGPVVGRSLAPKVEESPCHPVSLYGISKRAGEKVAEKYAKDLPITIVRPGIVFGEW
                     DKDCLEMFRPIARTGIHLVPGYRRKYYSLVYVGDLANLLIRAAEQGERLPANGQSGQP
                     GQGYYFAAYEETPSYAQLGKMMGEALGRRTLVIPVAPPVVWTVALFGEITGQLRKRPV
                     LFTWDKAREALAGSWICSAAKARRQLGFETPIALLDRLRQTVAWYRQAQWL"
     gene            complement(1026..1340)
                     /locus_tag="PKI05_08285"
     CDS             complement(1026..1340)
                     /locus_tag="PKI05_08285"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOA52242.1"
                     /translation="MDQLRQRRRTPRYAFPAVHWIAPIKGDERPTPADFIPVRCRDIS
                     RSGFSFWLEEPPDFRELVARIQHGTEILYVKAEVIHVTRPADGSTPGVVVGCRFEERI
                     SG"
     gene            complement(1563..2699)
                     /locus_tag="PKI05_08290"
     CDS             complement(1563..2699)
                     /locus_tag="PKI05_08290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOA52243.1"
                     /translation="MSRDRKVIVLTPPGRSAIATVRIEGVDALLDVCRYLSTRKGNPV
                     NITPAARPILARLKLPLGGSEEVVLHAASGECVEIHCHGSPLLVEQIVDLFVQMGYAR
                     ESVEAWIDRTESDIIAAEARRSLCEVLTFRGVQILIDQYRGALKGRVEVVQHLIQTKN
                     MEGTIELLDELLRWGELGLHLVKPWKVALVGPANVGKSSLLNRLVGYDRAIVDATPGT
                     TRDFVTAVTAIDGWPSELCDTAGLRQPQDPIEEEGVRRTLAVADAADLVVLVFSCADP
                     WSKDYDSWRERWPRSLVVYNKCDLPAAQGDRPAGLEVSAVSGHGLAELEQEVVNRLVP
                     VVPSPGTAVPFTDRQIRLLRQTRDAVLRGDYSEAEKHISTLLGH"
BASE COUNT          768 a          977 c          858 g          695 t
ORIGIN      
        1 attggctacc atctcataaa ggccctttca gaaggggatc atgagatccg ctgcctagca
       61 agaaagacct cgcgcatcga accgttgcgc ccattcgggg tccaattcgt ccaaggcgat
      121 attcgagatg ctgatagcat aacgggcgcg ctttccgacg tacagatcgt ttttcacctg
      181 gcgggaacga ccagggcgtt gcgtcgcgag gagtttttca gggtcaatga acacggcaca
      241 agacatcttt tagatgcctg cttgcggtgc aaggcaccac ccgttgtcgt atttgtctcc
      301 tcgctggctg ccgccggtcc cgtcgttggc cgatctttgg cgccgaaagt ggaagagtca
      361 ccctgtcatc ccgtctccct ctacggaatc agcaaacgcg cgggagaaaa ggtggcggaa
      421 aaatacgcca aggatttgcc catcacaatc gtccgccctg gaatcgtctt tggcgagtgg
      481 gacaaggact gtctggaaat gttccgcccg atcgccagga ccggcatcca tttggtgcct
      541 ggttatcgac gtaagtacta ttccctcgtt tacgtcgggg acctagccaa tctcctcatc
      601 agagccgccg aacaggggga acgcctcccc gctaatgggc aatctggtca acctggtcag
      661 ggatactact ttgcagccta cgaggaaaca ccctcgtacg cccaactagg aaaaatgatg
      721 ggcgaagcgt tgggcagaag aactctggtg attcccgtgg ccccgccggt ggtttggacc
      781 gttgcgctat ttggcgaaat aacaggccaa cttcgaaaac ggcctgttct tttcacatgg
      841 gacaaagcgc gggaggccct tgccgggtcg tggatctgct cagccgccaa ggcgcgccgt
      901 caattaggct tcgagacccc tattgccttg cttgaccggc tccggcaaac cgtggcctgg
      961 tatcgccagg cccaatggct ctaaagacat cagaagggat cagaacaagg gacacacgtc
     1021 ggcaattaac ccgaaatccg ttcctcgaat cggcatccga cgaccactcc aggtgttgac
     1081 ccgtccgccg ggcgagtcac gtgaatgacc tccgccttga cataaagaat ctcggtcccg
     1141 tgttgaatcc tagcgacaag ctcccggaaa tcaggcggtt cctcgagcca aaacgaaaag
     1201 cctgatcgag aaatatcacg gcaccggacg ggtataaaat ccgccggtgt cggccgttcg
     1261 tctcccttaa ttggggcgat ccaatggaca gccgggaaag cgtatcgcgg ggtacgacgg
     1321 cgttggcgaa gctgatccat gtgcgacacc atgcagaacg ccaaaaagta ctgtccctct
     1381 ccgaacactc ccatcatcca cccacggtca aattcgtgcc tcgaaaaaaa tatatcttgc
     1441 acttcgcaga gcggcataaa aagcaaatat aacttttctg gtcatctttg tcgtgttctc
     1501 cgctgggttg gatcccgtct tgaacagcgg ctaaggcaaa ccacaagacc cgagcggcag
     1561 gatcaatgcc caaggagcgt gctgatgtgt ttttccgcct ctgagtaatc gcctcgaaga
     1621 acggcatctc gggtctgtcg gagaaggcgt atttgccgat cggtaaaggg aacagcagtc
     1681 ccgggtgacg gaaccaccgg gacaaggcgg ttaacaacct cttgttctaa ctcggctagt
     1741 ccatgaccgg aaaccgccga gacttctagc cctgctggcc ggtctccttg agcagcagga
     1801 agatcacact tgttgtaaac cacgaggctc cttggccatc gctcccgcca ggagtcataa
     1861 tccttcgacc atggatccgc gcaactgaaa accaacacta ccaaatccgc cgcgtccgcg
     1921 actgccaagg tcctccgaac tccctcctcc tcgataggat cttgaggttg gcgcaagccc
     1981 gcggtgtcac acagctcgct tggccaacca tcaatcgccg tgacggccgt tacaaaatct
     2041 cgggttgtcc ccggagtcgc atcgacgatt gcgcgatcgt aaccaaccaa acgattcaga
     2101 agactacttt tgccaacatt tgcgggtcca acaagggcga ccttccacgg cttgaccagg
     2161 tgcagcccca actctcccca ccgcaacaac tcatcaagga gctcgatcgt tccctccatg
     2221 tttttggttt ggatcagatg ctgaacgact tcgacccgcc ctttcagtgc tccgcggtac
     2281 tggtcaataa ggatttggac accacgaaaa gtcagaacct cgcatagact tcgccgagcc
     2341 tccgcagcaa tgatgtccga ttccgtccga tcaatccacg cctcgacact ctcccgcgca
     2401 tagcccattt ggacgaacag atccacaatc tgctccacca gcaggggact tccatggcag
     2461 tggatttcta cacattctcc gctggctgca tgcaggacaa cctcctcgct gccccctaaa
     2521 ggtagtttca atctggccag aatgggtctt gcggccggtg tgatgtttac cggatttccc
     2581 ttacgggtgc tcaagtaacg acagacatcc aataaggcat cgactccctc gatgcgaacc
     2641 gtagcgatgg cacttcggcc tggcggggtg aggacgataa ctttccgatc acgactcatt
     2701 tcagaggtgg aaaaccatac taaaaaatga acccgttgtt gtgaccgtgt gattcttaag
     2761 ttactacggc aagaaaaagc caaccgtcgc gaacgtgggt aactcctacc ccatggtcca
     2821 gcgaccgacc agcgaacgca aagcatcgaa taccccgccg agccaccggg ccatcacacg
     2881 acatttatcg tagtggcact agatgacttt gggctagtac tcatggggaa aaggccagaa
     2941 ttggccgtga ctggcgttct agctgcctca gcaaacgaat cgaaacttga gggccggtgt
     3001 tatatgccgc gttctttatt tcttgagcat tcactcccat ttgccgatgt cgatagaata
     3061 gtaggggtag gctgcgcaag aaaaaagccg tgcccggcca gatgcgtcaa cgggtcgagc
     3121 aatcctactc gtttttcggc ccaccgtggc cagttgtagc cccttgtgag gagtgatcgg
     3181 gtgacacata ggggctacaa caccctaacg tctgaccgag cacggccagg attccgttcc
     3241 cctctcgtta gccgtcccta gcccgaaggg gtccatcccc gtttcgccgg cgaatgcg
//
LOCUS       DAOPTR010000387         3298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_23136_length_3298_cov_2.790626, whole genome shotgun sequence.
ACCESSION   DAOPTR010000387 DAOPTR010000000
VERSION     DAOPTR010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23136_length_3298_cov_2.790626"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..1232)
                     /locus_tag="PKI05_08295"
     CDS             complement(186..1232)
                     /locus_tag="PKI05_08295"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOA52244.1"
                     /translation="MRDDQWQALLELVSQGSTVPRIGLLVDSPWVPGWYGITIREYLV
                     SDRLWLQANLKAVEEFPDVWFLPGFWSEYGMCTEPSAFGGRCRFGVNDFPHIEPMMRS
                     WEEVRSLTPPDCTRDGLLPFVIERLKLTEDEMGKRGHRVRFAVSRGPLNIASYLLGQS
                     EFLLGLKAEKELAHQLLRMVTDFIKQWVRYQKEVFPSIDGLLILDDLIGFIGPKDFEE
                     FVIPYFTEIFAAINVPVRMLHNDAYGLITARHLEKMGANIFHFSCDHTFQQVRELCGP
                     NVVLMGNIKPRDVLALGTVDDLRREIQEAWHTAGDRTRILWSAGGAVPPNVSSEKIRV
                     FCEELRKLAGASSV"
     gene            complement(1509..2492)
                     /locus_tag="PKI05_08300"
     CDS             complement(1509..2492)
                     /locus_tag="PKI05_08300"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HOA52245.1"
                     /translation="MKAPDSSINVQKTFSLPVTNWTCRLVWTLWGVALVLMALGHTVT
                     TYGAGMVIPDWPTTYGHLPFLLPPKEWFEHWDIFLAYSHRLVAIISGLLSLVVAAAFW
                     RTRQNLHKWLATAAVFLVCLQVALGGLRVLTGDLLLVTIHACTGPVYFGLVTVLAVIA
                     GSHRKRPSLDEPSPVKTRKRASVVSIFVASLAYLQLVLIAQLRHVSPQLIPTAPLVIV
                     WAQVIIWGILGAVILGICLFLPGSCRAALGRMGLWLISGLYMVNVLLGILAWIVNFNL
                     PPRFAHGVLTVEYTVVQAGAAQAVLTTAFVLVTSLLFATSLVLVVSNWLRE"
     gene            complement(2508..>3298)
                     /locus_tag="PKI05_08305"
     CDS             complement(2508..>3298)
                     /locus_tag="PKI05_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOA52246.1"
                     /translation="RKGVFKYTSTVLGAKPTTEDLEKILRNGMPGTGMPSYKLLPDRD
                     IRRLVEYVKYLSMRGETELYLLRLVLDENELLPLNKQSVLEDGVLAVAESWQLPQSDP
                     DAWVVKPRPPILTTDGLKAAVERGRELYRDRRAKCATCHGPEGRGDGEQWDLYDDWNT
                     PKKGVTEQQTRDLARRFTLPIQRLRPRNFHQGIFHGGDAPEDIYLRIYLGIKGTPMPA
                     AGPAPGVPGVLTPDEIWDLTFYVLSLAGKLPQEITKNPSDGASP"
BASE COUNT          812 a          969 c          815 g          702 t
ORIGIN      
        1 tgatactccg gtccaataga actgccaatc atttggcaga aagcctggcc cgcgacacca
       61 atcaataccc accccatgag ccaggttttc caccaatgag cgaattgctg tctcagcata
      121 cccagtttgc ccaatcccgt tggacgctca gaggtaccct cttgggttat tgtcgttttg
      181 gcgacttaca ccgaactggc acctgcaagc ttcctcaatt cttcgcagaa tacgcggatc
      241 ttttcactgg acacgttcgg cggcactgcc ccacccgctg accaaagaat gcgtgtccgg
      301 tctccggccg tgtgccaggc ttcctgaatc tcccgccgaa gatcgtccac ggtgccaagg
      361 gctaggacat cacggggctt gatattcccc atcaagacca cattgggacc gcacagctcc
      421 cgcacctgct gaaaggtatg gtcgcacgaa aaatggaata tgttggcccc catcttttca
      481 agatgacggg ccgtgatcaa accataggcg tcgttatgca gcatgcggac ggggacatta
      541 atggccgcga atatctcggt aaagtagggg atgacaaact cctcaaagtc cttcggacca
      601 atgaacccga tgagatcatc aaggatcaat aatccatcaa tgctgggaaa tacttctttt
      661 tggtaccgca cccactgttt gatgaagtcc gttaccatcc gcagcagttg atgggcgagc
      721 tccttttcag ccttcagccc caagaggaac tccgactggc caaggaggta ggacgcaata
      781 ttcaacggtc ctcggctgac ggcaaaacgg acacggtggc cccttttgcc catttcatct
      841 tcggtcaatt tcaatctctc aataacaaaa ggcagcagcc cgtcccgtgt gcagtccggc
      901 ggcgtgagac ttcgaacttc ctcccaagac ctcatcatcg gttcgatatg ggggaagtcg
      961 ttgacaccaa aacgacatcg gccaccaaac gccgaaggct ccgtgcacat cccgtattcc
     1021 gaccagaacc cagggagaaa ccacacatcg ggaaactctt ccactgcctt caaattggcc
     1081 tgcaaccaaa ggcggtcgct gacgagatac tcccgaatgg tgattccata ccagccgggg
     1141 acccacggag agtcaacaag gagcccgata cgtggaaccg ttgacccctg cgaaaccaat
     1201 tccaacagcg cttgccattg atcatcacgc actggttcgc tccttaatct gggacccaag
     1261 gaaacgcaag ctaccttttt atgatactat ttctcacatc tcttcggaat gctccgagcc
     1321 gggattctaa tcctctcttc ttactcatac cgcaggatca ccggctgtca ttcaaccgtt
     1381 tcctgcacca gagacgaacc acagagtgcc ggtcatcccc ccgtctgtgc cgactttttg
     1441 cccgtcatca tcctcggcct ttgtcacgaa aaatcttcgt gtgaagcccc attttatgcg
     1501 gaggttagct attcacgcag ccagttcgaa acaactagga caaggctggt ggcaaaaaga
     1561 agagaagtga ccagaacaaa ggccgttgtt agcactgcct gtgccgcacc agcttgcact
     1621 accgtgtatt cgacggtgag taccccatgg gcaaaccgtg gcggcaggtt aaagttcaca
     1681 atccacgcga gaatccccag caatacattg accatataaa gccccgaaat gagccacagg
     1741 cccattcgtc ccagggcagc acgacaagac cctggcagga aaaggcatat tcccagaatc
     1801 acagcgccga gaattcccca aatgatcacc tgggcccaca caatgaccaa cggcgccgtt
     1861 ggtatcagtt ggggtgacac atggcggagt tgggcaataa gaacgagttg gaggtacgcc
     1921 aagctcgcca cgaagatcga gaccaccgat gcccttttgc gcgtcttgac cggcgacggc
     1981 tcatcgagtg aaggccgttt cctgtgagaa ccggcaatga cggcgagaac tgttacaaga
     2041 ccgaaataca ctggtcctgt acacgcgtgg atggtgacca agaggagatc acccgtgagg
     2101 acccgtaatc cccccaaagc gacttggaga cacactaaaa agaccgccgc agtggctaac
     2161 catttatgga gattttggcg agtcctccaa aaagccgccg ccacaacgag ggagagtaac
     2221 ccactgatga tggccacgag tcgatggctg taggcaagga atatatccca atgctcgaac
     2281 cattctttgg gcggaaggag aaatggcagg tgtccatagg tggtgggcca gtccgggatg
     2341 accatgccgg ccccgtaagt agtcacggta tgccccagcg ccatcaaaac cagcgcaaca
     2401 ccccacaggg tccaaaccag acggcaggtc cagtttgtga ccggcaacga aaacgttttt
     2461 tgaacattga tcgagctatc aggtgctttc atcacatacc cgcaacgtta tggtgatgcg
     2521 ccatccgacg gattcttggt aatctcttga ggtaatttgc ccgccagcga aaggacgtaa
     2581 aacgtcaaat cccaaatctc atcgggtgtc aacactcctg gaacacctgg cgccggccct
     2641 gccgcaggca tgggcgtacc tttgattccc aagtaaatcc tcaagtaaat atcctcgggt
     2701 gcgtcgcccc cgtggaaaat cccttggtgg aagttccgtg gtcgcaacct ctgaattggc
     2761 aaagtgaaac ggcgtgccaa gtctcgggtc tgttgctctg ttactccctt tttgggtgtg
     2821 ttccagtcgt cgtaaagatc ccattgctcg ccatcaccac gcccctctgg accatgacat
     2881 gtcgcacatt tggcccggcg gtctcgatac agttcccggc cccgctcaac ggcggctttc
     2941 aaaccatcgg tcgttaagat aggaggccga ggtttcacca cccacgcgtc aggatcactt
     3001 tgtggcaatt gccaggattc ggccaccgcc agcaccccgt cttcgaggac tgactgtttg
     3061 ttgaggggta acagttcgtt ttcgtcgagc acaagccgta agagatagag ctctgtttcc
     3121 cctcgcatac tcagatactt gacatactcc accaggcgac ggatgtcacg atcaggcaac
     3181 agtttgtagg agggcatccc cgtacccggc atgccgttac gcagaatctt ttctaagtct
     3241 tccgtcgtcg gtttggcgcc caataccgtt gacgtatact tgaacacacc tttgcgaa
//
LOCUS       DAOPTR010000388         3289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_23222_length_3289_cov_3.286333, whole genome shotgun sequence.
ACCESSION   DAOPTR010000388 DAOPTR010000000
VERSION     DAOPTR010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23222_length_3289_cov_3.286333"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1337)
                     /locus_tag="PKI05_08310"
     CDS             complement(99..1337)
                     /locus_tag="PKI05_08310"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HOA52247.1"
                     /translation="MLVVLRDRRFLLIFAILGWFALVSASLGRCGESSATELSGTRGD
                     RVEPRQAYQLLRVTPDGHGLLARHEGKTILVVSGTPEEMGKAHGSLLAPQIRYLVDRV
                     LYGVGAVDSIQTGIWFFELMDEIHRRTQPFIPERYFVEMDALARAAGLSQRDARYANL
                     FPERFHCSGVAVRGKASQNGRVIHARVLDYMRDINLQGCATIQVFVPEGRNAWISAGY
                     SGLIGTVTAMNEKGLAVGEMGGRGEGDWDGMPMTLLLREIMERAGTVQEAIEILKATP
                     RTCEYYYVLSDKTGELAAIRAVPQEVLVLGPGEQHPLLPHVPEDTVLISADERAKALS
                     QRLQLFYGKIDVATMIEIIKRPVAMSSNLHNAIMLPELLEMWVADAGQTTPACDEPYV
                     RVNLREILDFYRHVVSSRGG"
     gene            complement(1442..3175)
                     /locus_tag="PKI05_08315"
     CDS             complement(1442..3175)
                     /locus_tag="PKI05_08315"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase/BAAT C-terminal
                     domain-containing protein"
                     /protein_id="HOA52248.1"
                     /translation="MKQRRDVFPIAVLGCLLVWGFSGLSALALEQDLLNLQPGQAVAV
                     DETFDGKSFTYEVVDVQTKEHCLVVRLRYPSPVVSELPQNNVIPVEYYLPKGIQANGP
                     RRPAVICLHILDGSLELVRMLASVLASHGVPAVVFQLPYYGPRGDSDGPYRILADPQH
                     FVSVLEQAFLEVKRAVDFLASRPEVNPQHIGVAGISLGAIIAASAAERDPRIYKAGLI
                     LAGGNLRQIVETAREAEPLRRFIADLDADQQASIWKILDTVDPLSQADLLKERAQQGR
                     VLMINAAEDEVIPRACTEQLAAALGIADRVFWLNDLGHYTALAALPRILELTAEFFAA
                     DLPADVRVKETPSAEMTPVQLIAATLQRLTEFYLREPTEGACHIGQIRAHVNGDEYTF
                     LFIRGAGESFRLEGRASDFGQVMLGQRDYPWMMARTGLVFVGQPDAGGKPQSPLSFVN
                     NALLERARFAVLAVSGLSASPPALEALIRVKEEQQSDGRTIIVSPVDDTNQIAVRLKY
                     ARNQRFPNRLEVEGVGREIRVEIETWEIDVPAPVRLFDPPSDKPQKVVSRYDLYRMFG
                     AVINFLAEKAP"
BASE COUNT          773 a         1000 c          816 g          700 t
ORIGIN      
        1 gcccgacccc gtggagtttg aagtccgtag cggggatggg acatgccgtc tgtcctggga
       61 aaagcaatca cttgtggccg tgacaacctg agctcttatt aaccgccccg ggaagaaacc
      121 acgtgccgat agaagtcgag aatctcccga aggttgaccc gcacgtaggg ttcgtcgcaa
      181 gcgggtgttg tttgtcccgc atcggccacc cacatttcga gaagttccgg caacatgatg
      241 gcattgtgga ggttcgacga catggccacc ggtcgtttaa tgatttcgat catggtagcc
      301 acgtcgatct tgccataaaa aagctgaagg cgttggctaa gcgccttcgc ccgctcgtca
      361 gccgaaatga gaacagtgtc ttcgggcaca tgcggcaata aaggatgttg ttctccaggc
      421 cccagaacca gcacctcttg cggcaccgcc cttatcgctg ccagttcacc agtcttgtca
      481 cttaggacat agtagtattc acatgtccgc ggcgtggcct tcaaaatttc aatggcctcc
      541 tgaaccgttc cggcgcgttc catgatttca cgaaggagca gcgtcatcgg catgccgtcc
      601 caatcacctt cgccacggcc tcccatctcg ccgacggcaa ggcctttttc attcatggcc
      661 gtgactgtgc cgattaagcc cgaatacccg gcacttatcc aggcgttacg tccctcggga
      721 acaaatactt gaatcgtggc gcatccctgg agattgatgt cgcgcatgta atcaagaacg
      781 cgggcgtgaa tgacgcgacc attctggctt gccttacccc gaacggccac gccactacag
      841 tggaaccgct cggggaaaag attggcgtat cgtgcgtcgc gttgggacaa gcccgcagct
      901 cgggccaggg catccatctc cacaaaatac cgctccggta taaacggctg cgttcttcga
      961 tgaatttcgt ccattagctc aaaaaaccaa atgcccgttt ggatcgagtc aactgctccc
     1021 accccgtaca aaacgcggtc caccaaatac ctgatctgcg gcgccaaaag ggatccatgg
     1081 gccttgccca tttcttccgg tgttccgctc acgaccagga ttgtcttgcc ctcatgtctc
     1141 gccaaaaggc catggccatc tggcgtcacg cggaggagtt gataggcttg cctaggctct
     1201 acccggtccc cacgagtacc gcttaactct gttgccgagc tttccccaca tcgacccaat
     1261 gaggccgaca caagggcaaa ccaacccaaa atggcaaaaa tgagcaaaaa tctacgatcg
     1321 cgtaaaacga ccagcacggt tttcggtctc cttggtctgg gcccaaaaag aactagccaa
     1381 ttggttctcg agcataaatc cttatcgcat caacatgtta tcgttaccta tccgatgtcc
     1441 tttaaggggc tttctctgcc aaaaaattga tgaccgcccc gaacatgcgg taaagatcgt
     1501 accgtgaaac gaccttttgc ggcttgtcag agggaggatc aaaaaggcga accggtgccg
     1561 gaacgtcgat ttcccaagtt tcaatctcca cacgaatctc tcggccaact ccctcgactt
     1621 ccagccggtt tggaaaacgt tggtttcgcg catacttcaa gcggaccgcg atttgattgg
     1681 tatcgtcgac gggcgaaaca atgatcgttc gtccatcgga ttgctgttct tccttcacgc
     1741 gaatgagcgc ttcgagggct ggcggactgg cggacaatcc cgacacagcc aggactgcaa
     1801 atcttgcccg ttctaacaac gcgttgttta caaagctcaa gggcgattgg ggcttaccac
     1861 cggcatcggg ttgtccaacg aagaccaagc cggtcctagc catcatccaa ggataatcgc
     1921 gctgtcccaa cataacctgc ccgaaatcac ttgcgcgacc ttccagccga aagctctctc
     1981 ccgccccccg gatgaatagg aatgtgtatt catcaccatt tacgtgggcc ctaatttgcc
     2041 caatgtgaca agccccctcg gtaggctctc ggagatagaa ctcggtcaat ctctgaagtg
     2101 ttgccgcgat taactgtacc ggcgtcatct ccgcggatgg tgtctccttg acccgcacat
     2161 ccgcaggcag atcggcagca aagaactccg ccgttagctc taaaattcgg ggtaaagccg
     2221 ccaaggccgt gtagtgtcct aaatcgttca accaaaacac gcgatcggca atgcctaatg
     2281 ccgccgccaa ttgctccgta cacgcgcggg gaatcacctc atcctctgcg gcattgatca
     2341 ttaggacccg cccctgctgt gcccgctctt tgagcaagtc tgcctgcgaa agcgggtcca
     2401 ccgtatcgag gattttccaa atcgatgcct gctgatcggc gtccaaatct gcgatgaaac
     2461 gcctcagagg ttcggcttct cgcgcggttt cgactatttg acgcaaattt ccccccgcta
     2521 agattaaacc cgccttataa atccgcggat cccgctccgc cgccgatgcg gcgataatgg
     2581 ccccaagact gatccccgca accccgatgt gctgaggatt aacctcgggg cgagaagcca
     2641 gaaaatccac cgcacgcttg acctcaagaa atgcctgctc caacacgctt acgaaatgct
     2701 gaggatcggc gagaatccga tacggcccgt cactgtcccc gcgtggtcca taatagggca
     2761 actgaaacac aaccgccggg actccgtggg atgccaagac cgaggccagc atccggacca
     2821 attccaggct tccgtccaat atgtggaggc aaatcacggc cgggcgtcgt ggtccattcg
     2881 cctgaatacc cttaggaaga taatattcaa ccgggatgac gttgttttga ggtaactcag
     2941 aaacaaccgg tgacgggtat cgcaggcgaa cgaccaggca atgttccttc gtctgaacgt
     3001 caacgacctc gtaggtgaat gacttgccat cgaatgtctc atccaccgcc actgcttgac
     3061 ctggctggag gttgagcaaa tcctgctcaa gcgctagcgc agacaggccc gagaaccccc
     3121 acaccaacag gcatcccagc accgcaattg gaaagacgtc ccgtcgctgt ttcattgtgt
     3181 catgcacctc aaaccaccat cggctcaatc cgctaggccg cacctcgtcc caattaaaaa
     3241 caaatcctcg ccggccacgt gcaacagggt gtcaggttaa ccgagattc
//
LOCUS       DAOPTR010000389         3286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_23255_length_3286_cov_2.418756, whole genome shotgun sequence.
ACCESSION   DAOPTR010000389 DAOPTR010000000
VERSION     DAOPTR010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23255_length_3286_cov_2.418756"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="PKI05_08320"
     CDS             complement(<1..1200)
                     /locus_tag="PKI05_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52249.1"
                     /translation="MSPKTCLATVILVAITLALSCGVRAASPTSVEFRGEFQLPQIAF
                     ATDEILHAVMETASPQPQMVVEFQIDESLGPQAYRIERLKAPFGFRIVGGDATGAMYG
                     GVDVAEAIRIGTLNELAEGVRRPFIERRGIKFNIPLDVRTPSYSDNGDSFQANIPEVW
                     STAFWHEFLDEMARHRYNVLTLWNLHPFPSLVRVPEFPEIALDDVWRTRLRLDDTFSM
                     SGRDMVRPEMLQDVEIVRRMTIDEKIAFWRHVMQYAQDRGIEVYWFTWNIFVWGTDGK
                     YGITADRNNPVTIRYFRASVRELVRTYPLLAGIGITAGENMGRDGEGLTKEQWLWQTY
                     GEGIRDALAEQPGKQFRLIHRFHMTKLGEILQEFRNYPGPFDFSFKYSIAHMYSIPNP
                     PFIKSALE"
     gene            complement(1401..>3286)
                     /locus_tag="PKI05_08325"
     CDS             complement(1401..>3286)
                     /locus_tag="PKI05_08325"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HOA52250.1"
                     /translation="IPHEFQGAINNPLKGFRSYKKNAYGLIERRYIKWSDIEVCAGDT
                     VERIIAYTNKITEIDGKRFEDLNVKLVPRVYLDWNGTPGRQYWPADLQTFDYDSPAFQ
                     ERLRKLVEKLGQAWDNDPRIFAVQMGLIGYFGEHHNPAPTPQQRRLLVEAFQKAFKNK
                     PILVRHTDPEFMEAGFGIYYDTFGNLDREPPHGSKKQFPWQATHVYPDIWKLAPIEGE
                     VEYNWQNTRPSAKPDETFGRTPNETMKVPAFRRYMIDKIRRYHASYLGWISGYDDTDP
                     EVVAGASELQKVFGYRFVLDSVSYPLRIEPGDELVVKFTVRNTGSAPFYLDWPVAVGL
                     LNPVTKRPVWFAPLKGVDLRRWLPGEDWDSGAFAYRLPPRVYSEEGHAAVPEHIAPGR
                     YIVALAILDRQGGMLPSARFAVENYFRGGWHPFGYIGVGQSPGEPTLEGISFDSPAFD
                     DSLHYVVPEELRRVRTPPIPAIKPVTPWTPDPSRELINPWRYWTLDSGDTFLDKEVHH
                     DGPVEGPAGRRVIQVKGAFGPWATLNYEFPPNTPLLEGSYRLSFLVRGTPKESIEIEL
                     VENGRTVAKVGQVQLEAQWRSHTIEFKLAKKLDEKGSLRFRLPRNVEGSIELTDTHLK
                     RLE"
BASE COUNT          754 a          919 c          850 g          763 t
ORIGIN      
        1 ttctagtgcc gatttgatga agggcggatt cgggatggaa tacatgtggg cgatcgaata
       61 cttgaaacta aaatcaaacg gccctggata gttccgaaat tcctggagaa tctcacccaa
      121 cttggtcata tgaaaccgat ggatgagccg gaactgcttg cccggctgtt ctgccagggc
      181 atcgcggatt ccttcgccgt atgtttgcca cagccattgt tctttggtca agccttcgcc
      241 atcacggccc atgttctccc ctgccgtgat ccctataccg gccagaaggg gataggtccg
      301 caccagttcc ctgacacttg cgcgaaagta tcgaatggtg accggattat tgcggtccgc
      361 tgtgattccg tacttcccat ctgtacccca aacaaagata ttccaggtga accaatacac
      421 ctcgataccc cggtcctggg cgtactgcat aacatgacgc caaaaagcga ttttttcgtc
      481 aatcgtcatg cggcggacga tttccacgtc ctgaagcatt tcgggacgca ccatatcccg
      541 cccgctcatc gaaaacgtat cgtccaatcg caagcgggtt cgccatacat cgtccaaggc
      601 aatctcggga aactccggta cccgcacaag ggatggaaat gggtgaaggt tccaaagagt
      661 gagcacgttg tagcgatggc gcgccatctc gtcgaggaat tcatgccaaa atgcggtgct
      721 ccaaacctcc gggatattcg cttgaaaact gtcaccattg tcggaataac tgggcgtccg
      781 cacatcaagc ggaatgttga atttgatacc ccgacgttca atgaagggcc gacgcacgcc
      841 ctcggccaat tcgttcagcg tgccgatgcg aatggcctcg gccacatcca cgccgccgta
      901 catcgcgccg gtggcgtccc caccgacaat gcgaaaaccg aagggagctt tcagtcgttc
      961 gatgcgatag gcttgaggac caagcgactc atcaatctga aactcaacca ccatctgtgg
     1021 ttgaggcgat gccgtctcca tcactgcgtg caggatctca tcagttgcaa atgcaatttg
     1081 tggtagctga aactcacccc ggaactccac acttgttggg ctggcggctc ggaccccgca
     1141 agaaagcgcc aaagtgattg ccacgagtat cacggtggct agacaagttt ttggagacat
     1201 ctttacgctc ctccccacag tgccaatcgg ttttctcgtt ctgaacgctt gaaaacgacc
     1261 atattcatag agccggtaaa ttggaggact tccctcgaat ccttgcgagg gtattcctga
     1321 atcagcgtct cggttcgcaa taaatgactc ggcgatcaac tccttcccgg tcattcagac
     1381 ggataataga cacttagctc ttattctagg cgtttcaagt gggtgtcagt cagctcgatg
     1441 gagccttcga cgttccgcgg taagcggaat cgtaaggacc ctttttcatc cagctttttc
     1501 gccagtttga attcgatcgt gtgtgagcgc cattgtgctt caagctgaac ctggcccacc
     1561 tttgccacag ttcgtccgtt ttccactagc tcaatctcaa tagattcctt tggagttccg
     1621 cggacgagga aggacaaacg gtagcttcct tccaacagtg gcgtgttagg cggaaattca
     1681 tagttaaggg tagcccatgg tccgaaagcc cctttcactt ggataacgcg tcgtccggct
     1741 ggcccttcta ccgggccgtc gtgatgtacc tccttgtcca aaaaggtgtc gcccgaatcc
     1801 agcgtccaat accgccaggg attgatcagc tcgcgacttg gatcaggcgt ccacggcgtg
     1861 accggtttta tagcgggtat cggtggcgtg cgaacacgac ggagttcctc ggggaccaca
     1921 taatgaaggc tgtcgtcgaa tgcggggctg tcaaaagaaa taccctcaag agtgggttca
     1981 cccggagatt gaccaacgcc aatataacca aacggatgcc aaccaccccg aaaatagttt
     2041 tccacggcga atcgagcact gggaagcatg ccaccctggc ggtcaaggat ggcaagcgcc
     2101 acgatataac gtcccggtgc gatgtgctcc ggtaccgctg catggccttc ctcggaatag
     2161 accctgggcg gcaagcgata agcgaacgca ccgctatccc agtcttctcc gggcagccat
     2221 ctgcggagat cgaccccctt cagcggggca aaccagaccg ggcgtttcgt aaccgggttt
     2281 aagagtccga cagccacagg ccaatcaaga taaaatggag cgcttccagt gtttcgtaca
     2341 gtgaatttga cgacaagttc atcgccaggt tctatcctga gcggatagct cacggaatcc
     2401 aggacaaacc ggtagccaaa gaccttttga agctctgacg caccggccac cacctccgga
     2461 tcggtgtcgt cgtacccgct aatccagccg agataacttg cgtgatagcg gcgaatcttg
     2521 tcgatcatgt atcgccgaaa agctggtact ttcattgtct cgttcggcgt acgtccgaag
     2581 gtctcgtccg gtttcgcact cgggcgagtg ttttgccaat tgtactcaac ctcgccctcg
     2641 atcggagcga gtttccagat gtcagggtaa acgtgggtgg cttgccaagg gaactgcttc
     2701 tttgagccat gtggcggttc gcgatcgaga ttgccaaagg tgtcgtagta gataccaaac
     2761 ccagcttcca taaactctgg gtccgtatgc cgaacgagga taggcttatt cttgaacgcc
     2821 ttctgaaaag cctctaccag gaggcgacgc tgctgagggg ttggcgcggg attgtgatgc
     2881 tcgccaaagt acccgattag ccccatctgt acggcaaaga tacgcggatc attatcccat
     2941 gcctgcccca gtttttccac caatttcctt aaccgttcct gaaaagcagg actgtcgtag
     3001 tcaaacgtct gaagatcggc cggccagtac tgccttcccg gagttccgtt ccaatcaaga
     3061 taaacgcgcg gcacaagttt gacgttaagg tcttcgaatc gcttgccgtc gatttcggtg
     3121 atcttgttcg tgtaggcaat gatgcgctcc accgtatccc cagcgcacac ctcaatgtca
     3181 ctccacttga tatatctccg ctcaattagc ccgtaggcgt tcttcttgta ggagcgaaag
     3241 cctttgaggg ggttgttaat cgcgccttgg aactcgtgag ggatca
//
LOCUS       DAOPTR010000390         3284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_23276_length_3284_cov_3.748529, whole genome shotgun sequence.
ACCESSION   DAOPTR010000390 DAOPTR010000000
VERSION     DAOPTR010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23276_length_3284_cov_3.748529"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..1163
                     /locus_tag="PKI05_08330"
     CDS             105..1163
                     /locus_tag="PKI05_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOA52251.1"
                     /translation="MGNQPLDFEPGANWDVISDALGRTVEANLPLPTTLRALAADLDD
                     RRTASALKRIAARIERGESLDAIFSKEKSTKSRPVGQLLHAASLTPQPSETLNRFVAI
                     MEDYTANLRNLRTALSYPFLCLTLALFGAFVISAGWLLPNARDIVAMEEELSLTQPVR
                     LRIELMWAGNLVSLLIGIHVWGIGLLIILGPVRMSRFSLTYQLPMLGRYYAYLNLSLF
                     AKVMAHLTQSGVELSKALEAVAGLVSYPRLRRALKEAAAKAAESATFDEIVRTDQRFP
                     ATLRTFVTQSGSKLSLSDRFLAAADFFEDRARALWHAAPVLLMVFAILGLIMLWMTYA
                     IPLMMWIYWIQLLSGLAG"
     gene            1169..2410
                     /locus_tag="PKI05_08335"
     CDS             1169..2410
                     /locus_tag="PKI05_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52252.1"
                     /translation="MFYEKVAAMAGLCVLGLVLLDLSRTRNLPLSRQLSWGRVWLRIF
                     GWACLFVGGFFVGNVFLAGYSLVIILVVAAAVSGRTPSWGGQEIWVTIAAGIRHQLPI
                     PSLVEAAVEESGQAARYRQLLARLRAGMPLEELVRRLRIGRPERTALALATKTGRFDE
                     AIEKAREYRSAMWDVEELFTGRMLLLLLLLQGVPAGAFMFARISGHFGLILKDFGLDA
                     QTAATRFGLGVPAWFFSPGLLTYLWTAVSFGIVVGILLYWLGGRGILADFWPWSRCSL
                     RSRSLILMTLGQGVRSGLSFTEIFDVLVNHPPTLGIRGRLLRTLEAMFRGDDPWDAMR
                     KSGILSPADKDLLAASQQLGELPLSLETLARLHRDRYSAALRWWAVIGYLAIVTLFVL
                     GAMIAYACCLGTLLAIIFCLM"
BASE COUNT          654 a          848 c          971 g          811 t
ORIGIN      
        1 tgcggttggg ggaagccgcc tttggaaaat gatacccacg atgaatgatg tcctgtatgt
       61 ttggctgacg ccggtgtaat gttaggagac acgtggcggt agtcatgggt aatcaaccat
      121 tggatttcga accgggtgcc aactgggacg tgatctctga cgccctcggt cgaacagttg
      181 aggcgaactt accgcttccc acgacactgc gggccttggc cgccgacctg gacgacagaa
      241 ggacggcgtc agcgctcaag aggattgctg cccgaatcga gcggggcgaa tcactggacg
      301 ccattttttc caaggagaaa tcgacgaaat ccaggccggt ggggcaactc cttcatgcag
      361 cctctttgac cccccaacct tccgagacgc taaaccgatt cgtggccatc atggaggatt
      421 acacggccaa cctccggaat cttcggacgg ccctcagtta tccgtttctc tgtctcacgc
      481 tggcgctctt tggcgcattt gttatcagtg cagggtggtt gcttcccaac gcaagagata
      541 tcgtcgctat ggaagaggag ctttccctaa cccagcctgt caggctgagg attgaattga
      601 tgtgggcagg taatttggtt tctctcttaa tcggcattca tgtatggggc atcggccttc
      661 tgatcatcct cgggccggta cggatgtcac gtttctcgct gacttatcag ttacccatgt
      721 tggggaggta ctacgcctat ctgaacttaa gcctttttgc caaagtgatg gcccatttga
      781 cgcagtcggg cgtggaattg tcaaaggccc tggaagccgt tgcaggactc gtttcatacc
      841 cccgcctgcg gcgtgctttg aaagaggcag cggcgaaggc tgccgaaagc gcaacgttcg
      901 acgagatcgt gcgcaccgat caacgttttc cggcaacgtt acggacgttc gtaacgcaat
      961 caggaagcaa gctttcgttg tcggaccgct ttctggcagc ggcggacttt tttgaggacc
     1021 gggcacgcgc gctctggcat gccgcaccgg ttttattgat ggtgtttgcc atcctgggat
     1081 taattatgct ttggatgacc tacgccattc ctctcatgat gtggatttac tggatccagc
     1141 tgctttctgg cctagcggga tgacggtcat gttttatgag aaagtggcgg cgatggcggg
     1201 attgtgtgtg ttgggattgg tattgctaga cctgtcccgc acgcgcaatc taccgctctc
     1261 gagacaattg agctggggaa gggtatggct ccgcatattc ggatgggcat gtttatttgt
     1321 ggggggattt ttcgtcggca acgttttttt agcaggatat tcactggtta tcatcctggt
     1381 ggttgcggca gcggtttcgg gacggacgcc gtcttgggga ggacaggaaa tctgggtgac
     1441 gattgcagcc gggattcgtc atcaattgcc gattccatca cttgtcgaag cggccgtgga
     1501 agaatctggc caagccgcac ggtaccgcca attactggcg agacttcgcg ccggaatgcc
     1561 cctggaagag ctggttcgga ggttgcggat agggcgtcct gaacgaactg ccttggctct
     1621 cgccaccaag acgggacgat ttgatgaggc cattgagaag gctcgagaat atcgctcagc
     1681 aatgtgggat gtggaagagc tgtttacagg gcggatgctc cttctgctat tgctgttgca
     1741 gggggttccg gcgggtgcat tcatgttcgc aagaatatcg ggacatttcg gactcattct
     1801 gaaagatttt gggttggatg cccagacagc ggcaacccgt tttggcttgg gggttcccgc
     1861 gtggtttttc tcgccggggt tgttgacata cctttggacg gcggtcagct ttggtattgt
     1921 ggttggtatt ttgctttact ggcttggtgg tcgcggtatc ctggctgact tttggccatg
     1981 gtcgcggtgt tcgcttcgat cgcgaagtct gatcctcatg acattagggc agggggttcg
     2041 gtcagggttg tcttttacgg aaatcttcga tgtcctcgtc aatcatcctc ccacattggg
     2101 aattcgcgga cgactgttga ggacactgga ggccatgttt cgcggtgatg acccctggga
     2161 tgccatgcgg aaaagcggta tcctttcgcc ggcggacaag gaccttctgg ccgcgtcaca
     2221 acagcttgga gagttgccac tgagcttgga aacgttggcc aggttgcacc gcgatcgcta
     2281 cagcgcggcc cttcgttggt gggccgtcat cggctacttg gcgatcgtca cgttattcgt
     2341 cttaggagca atgattgcgt acgcatgttg ccttgggacg cttttggcaa ttattttctg
     2401 cctaatgtga gtagcttgac agccttgcgg gacggaaatg ttgctgagag tctatcccaa
     2461 agagtccccc ctctccctcc gggagagggt tggggtgagg gcaagcgaac gtgagaatcg
     2521 attgagaaaa ccggcagcgg ttcgaagcac cctcaccccc cgacccctct cccgccaaac
     2581 gggcgagggg agattatggg ataagctctt aacccgaagc aaaacgagcc gtgacgaccg
     2641 atacactgcc gatcaatgtc ccaccatgta tcgcgaccac cacggcacac agacgtccga
     2701 ttcagtggcc ccgcattggt acgggtaacc caccgtggtt gctcgtcgtc gaagatcgga
     2761 ccaattctga cggtctcaaa aagaaaaccg cccgagtcca agatggaccc cggcggttga
     2821 ccaatcgacc tctcgcgata ttgcgactgg atgcgtcgct gcgagacgaa cgagggtcgg
     2881 gcgtgcactt gcacaagcct ttgacttgac cctggttcac gctaaagggt tggcacctcc
     2941 gtcggaatat gcggcgagag gtccgattgt ctgcttcacc aacgattctt cctgcgtgga
     3001 ttgttatcgg ttacaatggc cgaaaaatcc actttcactt tttcacaggc cgacaactca
     3061 tcacatgaac ttgttggcca atcatcaata ttctacctta ttgccacgtg taagccaggg
     3121 caagaaacgt gctttttgaa gcgccggctg gcacaagtgt tgcacatcaa tccttcggca
     3181 ccagccagat attgcgatag gtcaccctat tgccgtgggc ctgcaaatgg atcggccgcg
     3241 gggccgggcc ttcttgctgg cgtcccggcg tggcccccgg caat
//
LOCUS       DAOPTR010000391         3282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_23301_length_3282_cov_2.913542, whole genome shotgun sequence.
ACCESSION   DAOPTR010000391 DAOPTR010000000
VERSION     DAOPTR010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23301_length_3282_cov_2.913542"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..1245)
                     /locus_tag="PKI05_08340"
     CDS             complement(226..1245)
                     /locus_tag="PKI05_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOA52253.1"
                     /translation="MVTQNRPQVLLSGFADESANHKTAIEQFVAFAALGLSYYSIRFI
                     DVGQGVKNVMKITPEELDKVIQLQQEYGLKVSSLGSPIGKIKLKDVEDGTKNVYIPFE
                     RYLQEDVARACELAQALGTKLIRGFSFYHPKGEDPRPYVTEVVDRLGQIADKCQQAGL
                     IFGLEVEANLVGQNGFLLAEIYDKLKHPALVLVFDGANLVVQGYTPGEIFEQYQAMKP
                     GLGWMHIKDYRRPQRDVKGGHVDEDMLKNFVPANMGDSGHEVILRDFATILPELEQKL
                     QAQGVPGVFLDLEPHVKGGGQFGGFSGPDGMGVALRGLCRLLDYVGISYHLRDFDDVR
                     AARGF"
     gene            1363..1644
                     /locus_tag="PKI05_08345"
     CDS             1363..1644
                     /locus_tag="PKI05_08345"
                     /inference="COORDINATES: protein motif:HMM:NF016245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HOA52254.1"
                     /translation="MGCFAWIHNPPFSVIHGQLCAFAKFKQWATAAAVPLNPTRREAK
                     VMQEVITAVVFAVFSTLYMRQPVNWNYAIAGLCLVVAAFFMFRGITHGA"
     gene            2167..>3282
                     /locus_tag="PKI05_08350"
     CDS             2167..>3282
                     /locus_tag="PKI05_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOA52255.1"
                     /translation="METLKTIGVLVVLLVVGYLGYKAVTNPPVTPPNETPTSQSPTVA
                     DATNVNIPIPELPPGMENQSADRSAKTSSLVSSPIVPPASSGGQVPTASLTARPNSSS
                     QEVAAQISNSGDLEGGLSSGQKSPDLSVIPPQPVVGSSFSARPSTEGAVPPIASPMGD
                     VRSGALATSGPAPEAESVSSQVMSQNAVTPPSSSSAVRPEFANFLEVACQKLDRGEFA
                     QVHQVLSAWYADPRLTPEEDAQLTDLLDQVAGTVVYSRQHILEPPYTVQPGDTLPQIA
                     ERFAVPWELLAKINGITQPDQLSPGMQLKVVRGPFEAVIRLQRRELVLMVQGRYAGRF
                     SIGLGRDAKGCEGVYQVREKTLNPRYYSDQGVIEANDP"
BASE COUNT          713 a          955 c          881 g          733 t
ORIGIN      
        1 ccacctcaac ccccagcccc cgcggagaac ccctttgcca cctgacctta gcgtgttgag
       61 atagggcccc aagggcaacc gcgaggccaa acaagtcact cacggcatga gtcgtaaaaa
      121 taaccgcagc cttcgcaggc tgcggtttct tttttggggc tacttccagg gagctagggt
      181 tttcgcacgg aaacctccac ctgcggacaa tgcaggacga aaacttcaaa atcccctggc
      241 ggctcggaca tcatcgaaat cccgcaaatg ataggatatg cccacatagt ccagaagccg
      301 gcacagaccc cggagagcca cgcccattcc gtcaggtccg ctaaatccgc cgaactgacc
      361 gccgcctttg acgtgcggtt ccaggtcgag gaagaccccc ggtacaccct gggcttgcag
      421 tttctgctct agttcaggaa ggatcgtggc aaagtcgcga agaatgacct catggccgga
      481 atcacccatg ttggccggta caaagttctt cagcatgtct tcgtcaacat ggccgccttt
      541 gacatccctt tggggacggc ggtaatcttt aatgtgcatc cacccgagac cgggcttcat
      601 cgcctggtat tgctcaaaaa tctctcccgg ggtgtaaccc tggaccacca ggttggcacc
      661 atcaaaaacc aaaaccagcg cggggtgctt gagcttgtcg tagatctcag ccaggagaaa
      721 gccgttttgt cccaccaaat tggcctccac ttccaggcca aagataagac cggcctgctg
      781 gcacttgtcg gcaatttgcc ccaaacgatc gaccacctcc gtgacgtagg ggcgagggtc
      841 ttccccttta ggatgataaa aggaaaagcc gcggatgagc tttgtcccca aagcctgggc
      901 taactcgcat gcccgcgcca cgtcctcctg gagataccgc tcgaacggga tatagacgtt
      961 tttcgtcccg tcctcgacat ccttcagctt aatcttgccg atgggtgaac cgagcgatga
     1021 gaccttcaac ccgtactctt gctgcagttg gatgaccttg tcgagttcct cgggagtaat
     1081 cttcatgacg ttcttgacac cctgacccac gtcaatgaag cggatactgt aataagagag
     1141 tcctaacgcc gcgaatgcaa caaactgctc gatggcggtc ttgtggttgg cagattcgtc
     1201 tgcaaatccc gaaagtagca cctggggacg attttgggtc accatttcac gcacctcatc
     1261 agttcgtttc tgggtggcca atataaggca gtcaacgtcc agccccggca ttctgcgttt
     1321 cccacggaaa acacttcctt tgccggataa gtccccagta ttgtgggttg ttttgcctgg
     1381 attcacaacc ccccgttttc tgttatccat ggtcagttgt gcgcctttgc caaattcaaa
     1441 cagtgggcaa ccgcggcggc tgttcctctc aatccaacga gacgtgaggc aaaggtgatg
     1501 caagaggtga tcaccgcggt cgtgttcgcg gttttttcca ccctttatat gaggcagccg
     1561 gttaactgga attacgccat cgcgggatta tgcctggtgg tggcggcctt tttcatgttc
     1621 cgaggaatta cccacggagc atgaccgaca tcaaagcctc cttaataaac ggggccaaat
     1681 gacgcaagcc tatgcttaga gcttatccca aaaagtcccc ctctccctcc gggagagggt
     1741 ggggggtgag ggcgagcgat cgtcggagcc gatcgagaaa accggaagcg attcgagtca
     1801 ccctcacccc caacatctct cctgcctaac gggtgaaaag agattatggg ataggctctt
     1861 attaggacta tccccggagt ccgtttcctt cctctcgaca tagatttggt caattgtaga
     1921 tatgccggct ggcaaggcac caccaacggg ggttattctt ggcattgggc tgcgcgcaat
     1981 tgctcttgga gaaaatctct attctgctta tcatgagtcc catcctctag gatggaagga
     2041 ctccgggtaa ttacgatagc cgcacgtccg gccgcaattg aggccgcgct gaccagtgag
     2101 tgactcaatg gccgagccac agtgtgggtt tgaatgtggc cctctgtggc gtcagggagg
     2161 acaggtatgg agacgcttaa aaccattggt gtgctggtgg ttctcctggt ggtgggttac
     2221 cttggctata aggccgtcac aaatccacca gtaacgccgc ctaacgagac ccccacttct
     2281 cagtcgccca cggtggcgga tgctaccaat gtgaacatcc ccattcccga actcccaccg
     2341 ggcatggaaa accaaagtgc ggaccggtcc gcaaagacct cgtccctggt cagttcgccg
     2401 atcgttccac ctgccagcag tggcggccaa gtcccgacgg cctcgctgac ggccaggccg
     2461 aattcctcgt cccaggaggt ggcggctcag atatccaaca gcggtgattt ggaaggaggg
     2521 ttgtcctccg gccaaaagtc tccggatctg agcgtcatcc caccgcagcc ggttgtcggt
     2581 agttcattta gtgctaggcc ttcaacggaa ggggccgttc cgccaattgc ctctcctatg
     2641 ggtgatgttc gcagtggggc cctcgccacc tcgggacccg ctccggaagc cgaaagcgta
     2701 tcctcccaag tgatgtcgca aaatgctgtt acaccgccgt cttcatcttc tgcagtccgc
     2761 ccagagtttg ccaacttcct tgaagtggcc tgccaaaaac tggatcgtgg cgaatttgcc
     2821 caggtgcatc aggttttgtc agcctggtat gccgatcccc gattaacccc cgaggaagat
     2881 gcccaattga ccgatctgct ggatcaagtt gcagggacgg tcgtttactc gcggcagcac
     2941 attcttgaac ccccttacac cgtccagccc ggtgataccc taccgcaaat tgccgagcga
     3001 tttgccgttc cttgggagct tctcgccaag atcaatggga ttacccagcc cgaccagttg
     3061 tcaccgggga tgcaactcaa ggtagttcgc ggtccgtttg aggctgtcat ccggctccaa
     3121 cggcgtgaac tggtgttgat ggtccagggc cggtacgccg ggcggttttc gatcggtctg
     3181 gggcgcgacg ccaagggttg cgaaggtgtg tatcaggtac gtgagaaaac cttgaatccg
     3241 agatactaca gtgatcaagg ggttatcgag gccaacgatc ct
//
LOCUS       DAOPTR010000392         3281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_23311_length_3281_cov_3.744575, whole genome shotgun sequence.
ACCESSION   DAOPTR010000392 DAOPTR010000000
VERSION     DAOPTR010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23311_length_3281_cov_3.744575"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PKI05_08355"
     CDS             complement(<1..310)
                     /locus_tag="PKI05_08355"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911191.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOA52256.1"
                     /translation="MIADKNPFNAAYDAETLIGKCRLYRLTTLEDLGLVRLSDLPYSI
                     RILLECVLRNCDGYQVTEEDVRNLANWASHSGKTEVEIPFKPARVLLQDFTGVPAIVD
                     L"
     gene            complement(488..1273)
                     /locus_tag="PKI05_08360"
     CDS             complement(488..1273)
                     /locus_tag="PKI05_08360"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOA52257.1"
                     /translation="MSQSQSWISFPGGTPEILYECKECLVVNKPRGILVQAPPGIPSL
                     AEMLRDFLATRQQPRTGIYLGVPHRLDRPVTGAILFTRHSRATRRITEQFESRMIRKV
                     YWAVVEGEVEPNVGIWRDFVRKIPNEPQAEIVPESHPDARHAVLNYRVLQRWENMTWL
                     EIHLETGRMHQIRLQASSRRHAVIGDWQYGSTIDLMPEETDPRSKPIALHARELTFLH
                     PMTFQTISVTAPLPSYWSRLSPLFLQYTSLSTSASGSETNLID"
     gene            1846..>3281
                     /gene="dnaK"
                     /locus_tag="PKI05_08365"
     CDS             1846..>3281
                     /gene="dnaK"
                     /locus_tag="PKI05_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOA52258.1"
                     /translation="MAQEKIIGIDLGTTNSVVAVMEGKEVKVIPNAEGNRLTPSVVAF
                     TDRGEILVGEPAKRQAITNPKRTVYSIKRFMGRRREEVKAEEAIVPYEIVGDVGEYVK
                     VKIGDRTYTPPEISAMVLRKLKEAAEAYLGHRVNKAVITVPAYFNDAQRQATKDAGQI
                     AGLEVVRIINEPTAASLAYGLDNKEAEKIVVFDLGGGTFDVSILEVAEGVFRVISTNG
                     DTHLGGDDFDRALVDYVAEEFKKEHGIDLRKDPMALQRLQEACEKAKKELSTLQQTDI
                     NLPFIAFDASGAKHLHMTITRSKFEQLVHHLIERCRGPVEQALRDARLTPKDIDEVVL
                     VGGSTRIPKVQELVREMFGKEPHKGVNPDEVVAVGVAIQGAVLAGEVKDVLLLDVTPL
                     SLGIETLGGVMTKIVERNTTIPCTRKQIFSTAEDNQTAVTIKVYQGEREMAADNRLLG
                     MFNLEGIPPAPRGVPQIEVTFDIDANGI"
BASE COUNT          777 a          836 c          963 g          705 t
ORIGIN      
        1 cgagatcaac aatggcgggc acgcctgtga agtcctgaag cagcacccgg gccggtttga
       61 aggggatttc gacctctgtt ttgccgctgt gactggccca atttgccaag ttgcgaacat
      121 cttcctcagt gacctggtag ccgtcacagt tcctcaacac gcattccaac aagattcgta
      181 tcgaataggg caagtcactc aagcgaacaa ggccaagatc ctcgagtgtt gtcagacgat
      241 agagacgaca cttcccaatc agtgtttcgg cgtcgtatgc ggcgttgaac ggatttttgt
      301 ctgctatcat ggacctagct cttcggaata attgagatga acgacaaata cctaggatgg
      361 gctacgaaag ctattaaacg gagcaacctc ctgccattga tgaccgttcc ggtatcctac
      421 tttccatggg acctcgtggg tagggcgggg agaaaccgaa gccaacgggt gaccgcgttg
      481 aaccccttca atcaatgaga ttcgtctctg aaccactggc ggaagtggac agagacgtgt
      541 actgcaaaaa aagcggcgaa aggcgactcc agtaagaggg caacggtgcc gtgacggaaa
      601 tggtttgaaa ggtcatgggg tgaagaaatg tcaactcgcg ggcatgcaaa gcgatgggtt
      661 ttgatcgggg atcggtttcc tccggcatta ggtcgatcgt actgccatac tgccaatcac
      721 caatgacagc gtggcgccga gaagaggctt ggaggcgaat ttgatgcatc cggccggtct
      781 ccagatggat ttccagccag gtcatgtttt cccagcgttg aaggactcga taattgagta
      841 cggcatgacg ggcatcggga tgggattccg ggacgatctc ggcctgcggt tcattgggga
      901 ttttacgcac aaaatcccgc caaatgccga cgttgggttc gacttcgccc tccactacgg
      961 cccaatagac ctttcgaatc atacgagatt caaattgttc tgtaatgcgt cgcgtggccc
     1021 gactgtgccg agtgaaaagg atggcgcccg tcacaggacg atcaagccga tggggaacac
     1081 caagataaat gccggtccgc ggctgttggc gcgttgccag aaagtcgcgg agcatttccg
     1141 ccaacgaggg aatccccggt ggggcttgga cgagaatccc ccggggtttg ttgactacga
     1201 ggcactcttt gcactcgtaa aggatctccg gcgttccccc gggaaaactg atccagcttt
     1261 gggattgcga catcgtgctc tgctgtccag gaatcatgtc taagatgata aatatcctaa
     1321 tttaacggac taagtgactc ccggtgggag atttcgtggc gagctagtgc gaagctatta
     1381 tcagcctacg ggcacccacc gacccatgcg ccacggcgag tcgggtggga gctcaatcgg
     1441 ccggcgatca gggtttctcc attggtttcg ccatcgccta ttccggcacc ccgcaaatta
     1501 ggcagggcgc tccgccaaat ttaggtggta ttgagccgcc cctatcttca tactgccaaa
     1561 ttggcagata ctcctcaatc gggcatagtt ccggctgcgg accataacac gtgcgtgctt
     1621 ataagtatcg gcaaatagtc aacttatgaa gcgtccctcc gctttttcgc tttccggcat
     1681 atcatttgct catgcggtat taacgtaagt ttttaccgaa ctcgtgatcg cgtcaatggg
     1741 ttgatgcatc tcggggattt ctaccaatac gaacatctcg cggaacgtgg ttttggtggg
     1801 taacggtccg caacaaggag agaatgcaga cagggagcta ttcacatggc acaagagaaa
     1861 attatcggta tcgacctggg aacgacaaac tcggttgtgg cggtgatgga ggggaaggaa
     1921 gtcaaagtca tccccaacgc cgaaggaaac cgactcacgc cgagtgtcgt cgcctttacg
     1981 gatcgcggtg agattctggt gggtgagccg gcaaaacggc aggccatcac caatcccaaa
     2041 cgcaccgtct attcgattaa acggttcatg gggcgtcggc gagaagaggt caaggctgaa
     2101 gaggccattg tcccctacga aatcgtcggg gatgtgggtg aatacgtcaa agtgaagatt
     2161 ggtgatcgga catacacgcc gcccgagatc tcggccatgg tcctccgtaa actgaaagag
     2221 gcggccgagg cctatttggg ccatcgggtg aataaggcgg tcattacggt accggcctat
     2281 ttcaacgatg cccaacggca ggccacgaaa gatgctggtc aaatcgccgg gttggaagtg
     2341 gtccgaatca ttaacgaacc gacggcggca tctctggcct acgggctgga caacaaagag
     2401 gccgagaaaa tcgtcgtgtt cgacttggga ggtggcacgt tcgacgtatc catccttgag
     2461 gtcgccgagg gtgtcttccg cgtcatcagc accaatggtg atacgcattt gggtggggac
     2521 gacttcgacc gcgccctcgt cgattatgtg gcggaggagt ttaagaagga acacggcatc
     2581 gacctgcgga aggatcccat ggcactgcaa cggctgcaag aggcctgcga aaaggctaag
     2641 aaggagctga gcaccttgca gcaaacagat atcaatttgc ctttcatcgc cttcgatgcc
     2701 agcggggcga agcacctcca tatgacgatc acgcgctcga agttcgaaca actcgtacac
     2761 catctcatcg agcgatgccg tggtccggtg gaacaggccc tgcgggatgc tcggttgacc
     2821 cccaaggaca tcgacgaagt cgtgctcgtg ggcggttcaa cgcggattcc caaggttcag
     2881 gaactagtcc gcgagatgtt tggaaaggag ccgcacaagg gcgtcaaccc ggacgaggtg
     2941 gtggccgttg gggtggccat tcagggggct gttctagctg gcgaggtcaa ggatgtcttg
     3001 ttgcttgacg tcacgccgct gtcgctgggt attgaaaccc tgggtggcgt gatgaccaag
     3061 atcgtcgagc ggaacacaac gattccttgt acccgcaagc aaatctttag cacggccgag
     3121 gacaatcaaa cggcagtgac gatcaaggtc tatcagggtg aacgagagat ggccgccgac
     3181 aaccggttac tgggcatgtt caatttggaa gggattcctc ctgccccgcg cggtgtgcct
     3241 cagatcgagg tcacattcga tatcgatgct aatggtatct t
//
LOCUS       DAOPTR010000393         3281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_23314_length_3281_cov_3.112213, whole genome shotgun sequence.
ACCESSION   DAOPTR010000393 DAOPTR010000000
VERSION     DAOPTR010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23314_length_3281_cov_3.112213"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PKI05_08370"
     CDS             complement(<1..376)
                     /locus_tag="PKI05_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52259.1"
                     /translation="MTPSPQKGTSLRPPSGLEFILGEVSSPPSFCVVYGDDDYLRTLV
                     LHKLRRHWLPDVEDEFCLREFDGNEVNWTAVEKELTTVSMFSSRPRVIIIEGADRFVA
                     EHRGELEKLLSGSPLRNVLMLSV"
     gene            complement(384..1043)
                     /gene="eda"
                     /locus_tag="PKI05_08375"
     CDS             complement(384..1043)
                     /gene="eda"
                     /locus_tag="PKI05_08375"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOA52260.1"
                     /translation="MMDNPHLSSILSCGICAVIRANSGAVLGDVAEALAEGGVTAIEV
                     TFTVPRAHRVLEAVADRLGDRIVLGAGTVLDPETARIAILSGAEFIVSPTLNVNVIEM
                     CKRYSKLVFPGAFTPTEILTAWQAGADIVKVFPSDVTGPGYLKAIHGPLPYIRLMPTG
                     GVTLETAADFIRAGACVLGVGSALVSSRAIAAKDFDQIRELARQYVNIVREARQGNTT
                     K"
     gene            1339..1578
                     /locus_tag="PKI05_08380"
     CDS             1339..1578
                     /locus_tag="PKI05_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52261.1"
                     /translation="MSRLLHSLILIVPGLFVVIVLVVLGTWWLQRLRRALYQDSSDSS
                     DELAQQFFKMRRDGQISEEEYKVIRANLSSQRRTS"
     gene            1857..3161
                     /gene="clpX"
                     /locus_tag="PKI05_08385"
     CDS             1857..3161
                     /gene="clpX"
                     /locus_tag="PKI05_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOA52262.1"
                     /translation="MPTGRDFTSGRRGPTSGKKNAFCSFCRKSYRDVGPLVEGPGDVY
                     ICGECIDLCQSILDQERKRKGTARPLFARVPTPREIVAKLDEFVVGQEEAKKVLAVAV
                     YTHYRRLMHHGTSDVVLDKSNILLIGPTGSGKTLLAQTLARLLDVPFAIGDATTLTEA
                     GYVGEDVENLLLKLLHAADFDIEAAQRGIVYIDEIDKIRKTSHNVSITRDVSGEGVQQ
                     ALLKMLEGTISNVPPQGGRKHPEQQYIQVDTTNILFICGGTFVGLEDIIARRMGKRSI
                     GFASSVPSSRRDREGGEANRDEYLLAHVTSEDLVEFGLIPEFIGRLPVVTALRPLDVD
                     AMVRVLVEPKNALVKQYEELFAMENAELEFTEEALRLIAQKAYEKGTGARGLRAIVEG
                     IMLDALYQLPDQPPGFRYVVDVDVVEGRKPLLGRPIARQKTA"
BASE COUNT          721 a          845 c          974 g          741 t
ORIGIN      
        1 tgacggataa catcagcaca tttcgaagtg gggaaccgga caatagtttt tccaactctc
       61 ctcgatgttc cgcgacgaag cggtcggcac cttcaatgat gatgacccta ggccgtgagc
      121 tgaacatgct cacggttgtc aactctttct cgaccgctgt ccaattgacc tcattcccgt
      181 cgaattcgcg gagacaaaat tcatcctcca catcgggcag ccaatgacgc cgaagtttat
      241 gcaagacgag cgtacgaagg taatcatcat ccccgtaaac gacgcaaaac gatggcggag
      301 atgagacttc gccgagaatg aattccagcc ccgacggcgg tcgcaaactc gttcccttct
      361 gcggactggg tgtcatgacg gcctcatttt gtggtgttgc cctgtcgcgc ttcgcgcaca
      421 atgttgacgt attggcgggc cagctcacgg atctgatcga agtccttagc ggcaatcgcc
      481 cgggaactca ccaaggcact accaaccccc agaacacaag cccccgcccg aatgaagtca
      541 gcagcggttt ctagtgtgac accccctgtc ggcataagtc gaatataagg cagggggccg
      601 tgtattgcct tcaagtaacc tggtcctgtg acgtcggacg ggaacacctt gacgatatcg
      661 gccccagcct gccaagcggt taggatttcg gttggtgtga acgccccggg aaaaaccaat
      721 ttgctgtagc gtttgcacat ttcgattacg ttgacattca gtgtggggct gacgataaac
      781 tcggctcccg aaagaatagc aatacgcgct gtctctgggt ctaggacggt tcccgcccca
      841 aggacgattc ggtcgcccag tcgatcggcg actgcttcga gcacgcgatg agcacgtggg
      901 acggtgaacg tcacttcgat tgccgtgacg ccgccctcgg cgagggcctc ggcgacgtcc
      961 cccagcacgg caccactatt ggcacggatg acggcgcaga ttccgcaaga aagaatactc
     1021 gaaaggtgtg ggttatccat catataaagc tcatcagcaa cttggttaaa ctgaggtact
     1081 tgcgacgctc ctcctgagct tattgcgatt cgcggggggt gtcaactcgg tcgccggttc
     1141 gaacatgcgg aagggtcata accgccgaaa gtcctcctgt cgctggacga tcgtcctaaa
     1201 ccgcgcgata atgatgacgg accgccgagg ctgagatgcc cggtcgtcac gtctcgacgg
     1261 cggttcgttc ctcatgcgaa tgtcacgttg agcggagtct gacacccatt tggaccgttt
     1321 gaggagagac ttggcgggat gtcacgtctg ctgcactcgt taatccttat tgtaccagga
     1381 ctttttgttg ttattgttct cgttgtgctc ggaacgtggt ggcttcagcg tctgagacgt
     1441 gccttatacc aggactcaag tgacagtagt gacgaactgg cgcagcagtt ctttaagatg
     1501 cgcagggatg gtcagattag cgaagaggag tacaaggtaa tccgggcgaa cttgtcgtca
     1561 caacgacgca cgtcgtgatg aagcgaaaac tgggcttcgg ggctatgtgg tagaatttag
     1621 gtaaaagacg aaggttgcgt gaaactcttc gggcgatgcg tcgcgtaatg attagcgaaa
     1681 gggaggacac acgccgcgtt gaacggcggt ttttgtcccg atatcgccga tgaccaatca
     1741 atgtcagggg aatgactgag gattgccagc ggcgatcatc tccgtagggg agggcagggg
     1801 ggctggcggc ttggacttcg acgtttcaac tttttgtgac acccagggtt tgaagcatgc
     1861 cgacaggacg ggacttcaca tcaggacgcc gtggtccaac gagtggcaag aaaaacgcct
     1921 tttgttcgtt ttgccggaaa agctaccgtg atgtaggacc gttggtcgaa ggaccagggg
     1981 atgtttacat ctgcggggag tgtatagacc tttgtcaatc cattcttgat caggaaagaa
     2041 agcgcaaggg aacagcccgc ccgctttttg cgcgagtgcc aacccctcgc gagatcgtcg
     2101 ctaaacttga cgagtttgtt gttggccaag aggaggccaa gaaggtcctg gccgtcgccg
     2161 tgtacacgca ttaccggcgg cttatgcacc acgggacttc ggatgtcgtg ttggacaagt
     2221 caaatatcct cctgattggt cccacggggt cgggaaagac gcttttggcg cagacgcttg
     2281 cccggctgct ggatgttccc tttgctattg gcgatgcgac aaccctaaca gaggcgggtt
     2341 acgttggaga agacgttgaa aacttgctcc tcaagttgct gcatgcggcg gactttgaca
     2401 ttgaggcggc acagcgcgga atcgtctata tcgacgaaat tgacaagatt cgcaagacga
     2461 gtcacaacgt ctccattacc cgggacgtct ctggtgaggg ggttcagcag gccttgctta
     2521 agatgctcga agggactatt tccaatgtcc caccgcaagg cggacgaaag caccccgaac
     2581 agcaatacat ccaggttgat accacgaata tcctctttat ttgtgggggt actttcgttg
     2641 ggctggaaga cattatcgcc aggcgaatgg gaaaacggtc gattggattt gcctcatcgg
     2701 taccgtcatc ccgtcgggat cgcgaaggtg gcgaggcgaa tcgggatgag tacctcttgg
     2761 cccacgtgac gagcgaagac cttgtcgagt ttggcctgat tcccgagttt attggccgat
     2821 tgcctgtggt gacggcgctt cgcccccttg atgttgacgc catggtgcgt gtcttggtcg
     2881 agccaaagaa tgctttggtc aagcagtacg aagagctttt cgccatggaa aacgcggagt
     2941 tggaattcac cgaggaggcg ttgcgcttaa ttgcccaaaa ggcctacgag aagggcaccg
     3001 gtgcccgagg tctgcgggcg attgtggagg gtattatgct cgatgccctc tatcaattgc
     3061 ccgaccaacc gccgggcttc cgctatgtgg tcgatgtgga tgttgtggaa gggagaaaac
     3121 ccctgttggg tcggcccatt gcccggcaaa agacggcctg agaggccttt tgacgcgatc
     3181 acaggtcgtt tcgtttctaa ggttctgctt aaagcctatc ccctagtctc ccctcgcccg
     3241 ttcggcggga gagggggcgg gggtgagggt ggcttgaatc g
//
LOCUS       DAOPTR010000394         3275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_23375_length_3275_cov_3.207143, whole genome shotgun sequence.
ACCESSION   DAOPTR010000394 DAOPTR010000000
VERSION     DAOPTR010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23375_length_3275_cov_3.207143"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PKI05_08390"
     CDS             <1..327
                     /locus_tag="PKI05_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52263.1"
                     /translation="FGRPPRDNGLESERNNRITAGQRLHLLNSSHIARKIEDGRGLEW
                     FFASSGNPRAKVTRLYLTVLSRYPTEKELAVIAETAKAQRSLSRQAGVDLVWALINSS
                     EFLYRH"
     gene            343..1746
                     /locus_tag="PKI05_08395"
     CDS             343..1746
                     /locus_tag="PKI05_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HOA52264.1"
                     /translation="MSDFRLAHVILNPADQAGPREKLTRRTLLARGLFGVLGLYLGHR
                     GFTSPSAWAFDPLLPADKPGKAKAVIQIWLWGGPSHLDTFDPKPDAGNDYTGPFTNPI
                     STNVPGIRINELLTELAKVADKYSLIRGMTHGVNAHETAAYLVQTGRMPGDRIVAPAV
                     GAVVSYFKGQPPEYQSLIPPYIVLTEPQGRFSEAGFLGPKYKPFATGGDPSKTPFTVE
                     GIVAPGMTQSRQRERRELLAKLDILRQTVSDHPQIQELKQAEEQAYDLILGSSGKAFD
                     PSEEPQELRQKYGTTTFGQSCLVARRLVERGVRYVTINSKGWDTHKEHFQAMRRKLPD
                     LDRGLATLIQDLADRGLLESTLIWCCGEFGRTPKIAWEPPWNGGRHHYGKAFSVLVAG
                     GGFKGGQVVGATDKTGSEVVERPVYPTDLIGSIYTLLGIDPATTVRHPQGQMVRIVPT
                     PEEGAPSAGLLKEIMTV"
     gene            1866..>3275
                     /locus_tag="PKI05_08400"
     CDS             1866..>3275
                     /locus_tag="PKI05_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HOA52265.1"
                     /translation="MSRMKRHQTLLSLGILVAFWSFWTPVQGQQVPSSMRLPHVGYVY
                     FAGGQAGTEFDVTIGGQYLDGISEVLISRLDPLGSMESGVPRGTFVKLSKPLTQKQVN
                     DLSEKLKDIQARVREEMRRRRASVGQYRELFVKFAAEAGITEEMLKELDEFRKNRNDP
                     KRQPAPQIAERATIHIVLPKDTEPGLYLIRLRTRTGVSEPLRFQVTPWQEVLENEPND
                     HGQVHTVTEGLPVVLNGQIMSGDVDRFQISAKKGQHLVVAVAARELIPYLADGVPGWF
                     QATLRVLDDSGREVAYVDDFWFHPDPALSFEVPRDGDYIIEIKDAIYRGRQDFVYRIV
                     VGEIPFVTSIFPLGGRLGEKCQVTLNGWNLPQREWTLAPLKGDPGIRQILLPQWAAMP
                     RSVSFAVGQWPEVLEQEPNDGRANAQKLITGVVVNGHIDKPDDRDVFVIEGRAGQNIV
                     AEVLARRLYSPVDSILRIID"
BASE COUNT          712 a          865 c          980 g          718 t
ORIGIN      
        1 tttgggcgac cgccacggga taacgggctg gagtccgaac gaaataaccg gatcacagca
       61 ggacagcggt tacacttgct caattccagt catattgccc gaaaaattga agacggtcga
      121 ggtttggagt ggttctttgc atcttcggga aatccgcggg ccaaggttac gcgtctgtac
      181 ctgacggttc tctcacgata cccaacggaa aaggagttgg ccgtcattgc ggagacggcg
      241 aaggcccagc gcagtctttc ccgccaggcg ggggtggacc ttgtctgggc cttaatcaat
      301 agttctgaat tcctttatcg ccactaaata cggaggcggt gtatgagcga ctttcgactc
      361 gcccatgtaa tcctcaaccc agcagatcag gctggacctc gcgagaagct gacccggcgg
      421 accctcctgg cacggggtct gttcggcgtt ttgggactgt atctaggcca tcggggtttc
      481 acgtcaccat cagcatgggc gtttgatccg ctccttcccg cggataaacc cggcaaggcc
      541 aaagcggtga ttcaaatctg gctgtggggc ggcccatcgc atctcgacac gtttgatccc
      601 aagcccgatg cggggaacga ttacacgggt ccctttacga accctatctc gacgaacgtg
      661 ccaggcatcc ggatcaacga gctcctcacg gaattggcaa aggttgccga caagtatagt
      721 ctgatccggg ggatgaccca cggcgttaat gcccatgaga cggcggccta tttggtgcag
      781 actggccgca tgccgggcga ccgcattgtt gccccggcgg ttggagcggt cgtcagttac
      841 ttcaagggac aaccgcccga atatcaaagc cttattccgc cttatatcgt ccttacggaa
      901 ccgcaaggtc ggttttcgga ggccgggttc cttggcccga aatataagcc ttttgcgacc
      961 ggaggcgatc cctcgaaaac tccgttcacg gtagagggga tcgtggcccc tggcatgaca
     1021 cagagccgtc aacgggaacg gcgcgagctc ttggcgaaac tcgatatctt gcggcagacg
     1081 gtcagcgatc acccccagat tcaagagcta aagcaagcgg aagagcaggc ctatgatctc
     1141 attttgggtt cgagcggtaa ggcgtttgac ccgagtgaag aaccgcaaga gctcaggcaa
     1201 aagtacggga caacgacatt tggccaatcc tgcttggtgg cacgccgcct agtcgagcgt
     1261 ggcgtccgtt atgtcaccat taattccaaa ggttgggata cgcataaaga acacttccaa
     1321 gcgatgcgcc gcaagctacc cgatctcgac cgcgggctgg caacgctgat ccaagatttg
     1381 gcagatcggg ggctgttgga aagcaccttg atttggtgtt gtggcgagtt tggacggacc
     1441 cccaagatcg cctgggaacc gccctggaac ggaggacggc atcactatgg caaggccttt
     1501 tccgtcctcg tggctggcgg aggcttcaag ggtgggcagg tggtcggggc aaccgacaag
     1561 acgggttccg aggtcgttga acggcctgtc tatccgacgg atctgatcgg gagcatctac
     1621 acgttgctgg gaatcgatcc ggcaacaacg gttcgacatc cccagggcca gatggtacgg
     1681 attgtgccaa caccggaaga aggtgctccc tcggccgggt tgcttaaaga gattatgaca
     1741 gtttaacagt ttaacccatc gagcaatccg atccgttggg tgaagtgcgg tcaatagtgg
     1801 gttggaatcc ggcgcgtagg gacgagtgcc ggaaaaaatc gtgttttcgt tgattaggtg
     1861 cgatcatgtc tcggatgaaa cgacatcaga ctcttctttc gttgggcatt ttggtcgcct
     1921 tttggagttt ttggacgcca gttcaggggc aacaggttcc aagcagcatg cgattgcccc
     1981 atgtggggta cgtttatttt gccggggggc aggccgggac cgagtttgat gtcaccattg
     2041 gtgggcagta tctcgacggg atttcggaag tcctcatttc ccgccttgac ccactcggtt
     2101 caatggaaag cggggtaccc cggggaactt ttgtcaagct gtccaagccg ctgacacaaa
     2161 aacaggtcaa cgatctttcg gagaagctta aggacatcca agcccgggtc cgcgaagaga
     2221 tgcgacgtcg gcgagcatct gtaggacaat accgggagct ctttgtgaag tttgctgctg
     2281 aggcggggat tacggaggag atgctcaagg aacttgatga attccgaaaa aaccgcaatg
     2341 atcccaagcg gcagccggca ccgcaaattg ccgaacgggc aaccattcac atcgtccttc
     2401 cgaaagacac cgagccgggg ctgtatctca ttcggcttcg cacccgaaca ggcgtgtctg
     2461 aaccgctgcg gtttcaggta accccgtggc aggaagtcct tgagaacgag ccgaacgatc
     2521 atggccaggt gcacacggtt acagagggct tgccggtcgt tctcaatggt cagatcatgt
     2581 ccggtgacgt ggatcggttt caaatctcgg cgaaaaaggg gcaacatctt gtggtggccg
     2641 tggccgctcg cgagttaatc ccctatcttg ccgacggtgt gccgggatgg tttcaggcga
     2701 ccctccgtgt gctcgatgat tccggtcggg aagtggccta tgtcgatgat ttttggttcc
     2761 atcccgatcc ggcgctgagc tttgaggtcc cacgagatgg ggattacatc attgaaatca
     2821 aagatgccat ctatcgtggc cgtcaggatt ttgtgtaccg gattgttgtg ggagagattc
     2881 cctttgtcac cagtattttc ccactcgggg gccgtttagg ggagaaatgt caagtcactc
     2941 tgaacgggtg gaacctgcct cagcgggagt ggacacttgc gccgctcaag ggcgatcccg
     3001 gcataagaca aattcttctt ccccaatggg cagcgatgcc tcgctcggtg tctttcgccg
     3061 tgggccaatg gcctgaagtt ttggagcaag agcccaatga cgggcgcgct aacgcccaaa
     3121 aactgatcac cggcgtggtg gtcaatggac atattgacaa acccgacgat cgcgacgttt
     3181 tcgtaatcga ggggcgcgct ggtcaaaaca tcgtggctga agtccttgcc cggcgtctct
     3241 attcaccggt ggattcgatc ctccgcatta tcgac
//
LOCUS       DAOPTR010000395         3270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_23426_length_3270_cov_2.818663, whole genome shotgun sequence.
ACCESSION   DAOPTR010000395 DAOPTR010000000
VERSION     DAOPTR010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23426_length_3270_cov_2.818663"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PKI05_08405"
     CDS             complement(<1..585)
                     /locus_tag="PKI05_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007336872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOA52266.1"
                     /translation="MKQTVHWLRGMTQFFRGKGSGHLAGKERVSSRQSQEGQQSRARM
                     GKNRPDSQEGAPGREENTRASNWLREVVEAISIALILAFLFRAFEAEAFVIPTGSMAP
                     TLMGRHKDLICERCGFSFQTGASEEVDKLTNRPTGRFVLSATCPNCRYTMYVGPNKDD
                     GPPTPSYKGDRILVSKYEYHLSEPRRWDVIVFKYP"
     gene            complement(840..2426)
                     /locus_tag="PKI05_08410"
     CDS             complement(840..2426)
                     /locus_tag="PKI05_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52267.1"
                     /translation="MELHSRDALMCRCCANRRRFLKSCAGCGVAALAASSLRLAPAES
                     QESSAIPASSDRVRVRLVFACWALVQDRPTWPHIGHDMRPEIERVTNALREGCPEIEF
                     LPVAAHSVEDADKLLAQHDADKIDGYLVCNMNNWIQVFHRIAASGHPMLLADFIYAGS
                     GGFLVYGSQLRRQYRNFSLVASSDISDLIAAAKCFTILKSGGVEQFVAACDRIRQERT
                     PKPRIQKPAEDRLTLKSVGQCLEEVKNSGIISIGGGNKGVAEAIQQQLGIPVHFVSFE
                     EMAEAAEAADKAQVDELAEKWRKEAQKVLIDEPEETLRLSAANYLAQKALLNKYRAEA
                     ITINCLGGFYSGKLKAYPCLGFVELLNSGFVGACEADLPSTVTMVMVNHLAGRPGYIS
                     DPVIDTSKREIIYAHCVATTKPFGPEGPANPYEILTHSEDRRGASVRSFLPVGYMTST
                     MEIFPDRKQILFHQGVAAENVVIDRACRTKLGCEVWGDLDKLLLGWSNGWHRVTFYGD
                     LKDAVKELAAALQFNLVEEA"
     gene            complement(2731..>3270)
                     /locus_tag="PKI05_08415"
     CDS             complement(2731..>3270)
                     /locus_tag="PKI05_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOA52268.1"
                     /translation="DNTALLVIDVQERLIPHITDHKRIVWNIRRLIDAATILGVRVVG
                     TEQYPKGLGPTVPVLAERLGTLPEKLMFSCRECWNLFNELKAQGMRNLLVTGIETHVC
                     VQQTVLDFLAQGWGVYVAADAVGSRHRIDYEIALRRMEVSGAVLTTTEAALFEWCVRA
                     GTDQFKKISRLVQESLPDE"
BASE COUNT          764 a          952 c          835 g          719 t
ORIGIN      
        1 cgggtatttg aagacgatta catcccagcg gcgtggctct gaaaggtggt actcgtattt
       61 tgacacaaga atgcgatcgc ccttgtacga cggagtcggt ggaccatcgt ccttgttcgg
      121 accaacatac atcgtgtagc gacaattagg gcacgtggca ctcaagacga aacgtcccgt
      181 gggtcgattc gtgagcttgt caacctcttc gctagcaccc gtttgaaaac tgaagccgca
      241 gcgttcgcag atcaagtcct tatggcgccc catcagtgtt ggtgccatgg accccgttgg
      301 aatcacgaat gcctccgcct cgaatgcccg gaagaggaac gccaaaatta gagcgatcga
      361 gatcgcctca accacctcgc gaagccagtt ggaggcccgt gtgttttcct cgcgcccggg
      421 agctccctct tgggaatccg gccggttttt gcccattctt gcccgtgact gttgtccttc
      481 ttgagattga cgagaactaa cccgctcttt cccagccaaa tggcccgaac ctttacctct
      541 aaagaactgc gtcatcccgc gcagccaatg caccgtttgc ttcatttcgc ttacactttc
      601 aaaatcgatg tatcaattac ggccaaaaaa cgaacgtacc tggcaaaatg ctccaggaag
      661 gccatgggat cgccacggca gaacctaaat gttaatgtaa caaatctctg ccaacctaag
      721 cagatcacat ttggtggcag ctttcttctg cttggggccc cgcgacacct tttagatgtc
      781 aagctactga accgaagatg tgggccactt tgagtgcccg tttagtgggg taacgcgtat
      841 caagcctcct ccaccaggtt aaactggagg gcagccgcca actccttgac ggcgtccttc
      901 agatcaccat agaaggtgac gcgatgccac ccgttgctcc accccaataa cagcttgtcc
      961 aaatcccccc aaacttcgca ccccaatttg gtgcggcatg ctcggtcaat gaccacattt
     1021 tccgccgcga caccctggtg gaacaaaatc tgtttgcgat cggggaagat ttccatcgtg
     1081 gacgtcatgt accccacggg gaggaaggat cgtactgagg ccccccgtcg atcctccgag
     1141 tgagtcagga tttcgtaggg attggcaggc ccttctggtc caaacggctt cgtcgtggcc
     1201 acacagtgag cgtaaatgat ctcccgcttg gaagtgtcaa tgacggggtc agagatgtac
     1261 cccgggcgcc cggccaagtg attaaccatg accattgtga cggtcgaagg gaggtcggct
     1321 tcgcaagcgc caacaaatcc gctattgagc agctcgacaa acccaagaca cgggtaggcc
     1381 ttgagttttc cgctataaaa cccacctaag caattgatag taatggcctc ggcacgatac
     1441 ttgttcaaca gagccttttg tgccaagtag ttggccgccg agagccttag ggtttcctcg
     1501 ggctcgtcaa tcaagacctt ctgggcttct ttgcgccatt tttcggccaa ctcatcgact
     1561 tgggccttgt ccgccgcttc ggcagcctcg gccatttctt cgaagctgac gaaatggacc
     1621 gggattccga gttgttgctg aatggcctct gcaacaccct tatttccgcc cccaatcgag
     1681 ataataccgc tgttcttgac ctcctccaaa cactggccga ccgatttgag tgtcaaacga
     1741 tcctcggctg gtttttgaat ccgcggcttc ggtgtccttt cctggcgaat tcgatcacaa
     1801 gcggctacaa actgttcgac tcctcctgac ttgaggatgg tgaagcattt tgcggccgcg
     1861 atcaagtcgc tgatgtcgga agaagcaacc agagagaagt ttcggtattg gcgccgcagt
     1921 tgggacccat agaccaaaaa acctccgctt ccggcgtata taaaatcagc cagcagcatg
     1981 gggtgcccgg atgccgcaat tctgtgaaag acctggatcc agttgttcat gttgcaaacc
     2041 agatagccgt cgatcttgtc ggcatcgtgt tgggccaaga gcttgtcggc atcttccaca
     2101 ctatgggcag ccaccggcag aaactcgatc tcagggcacc cttcccgcag cgcattggtc
     2161 acacgctcaa tctccggtct catgtcgtgt ccaatatggg gccaggtggg ccgatcttga
     2221 actaatgccc agcaggcaaa aaccagtcgc acccgaaccc gatcagaaga cgcaggaatt
     2281 gccgaacttt cctgcgattc agcaggagca agtcggagcg aagacgccgc cagcgcggct
     2341 acgccgcatc ccgcgcaact tttcaagaag cgacgacggt tcgcgcagca acgacacatg
     2401 agagcatcgc gagaatgcaa ttccatgatg ggacctccct tactgatggt tgtcggaact
     2461 caaccaacgc ccaaaatatg agagacgtgt tcgcggcagg cggccaagct gcaaagatct
     2521 ccactgtaca acgaccaccc acatcgataa tcctcaccca gtacaaccag tctacacact
     2581 tcagggtggc atgactgtca agtgacttgg ccatattttg tcaaaatttt tggagaaacg
     2641 caagcaagag aaaattgggc gcgtttcaaa aaagtcaccc ccaaaaggca accccacatg
     2701 ccccggcgag aaacttgggc cgccgcaggg ctactcgtct gggaggctct cctggaccaa
     2761 gcggctaatt tttttgaact gatcggtacc ggctcggaca caccactcaa aaagggccgc
     2821 ctcagtcgtc gtcagcacgg cgccggatac ttccatccgc cgaagggcga tttcgtaatc
     2881 tatccgatgc cttgacccaa cggcgtcggc ggcaacatag acaccccatc cctgtgcgag
     2941 gaaatcgagg acggtctgct gaacgcatac gtgggtttca atgccggtta ccaagagatt
     3001 ccgcataccc tgtgccttca actcgttgaa gagattccag cattcgcggc aactaaacat
     3061 cagtttctcc ggtagcgtcc caagacgttc ggccaagacg ggcacagtcg gccccagccc
     3121 cttggggtat tgttcggtgc ccacgactcg cacaccgaga atcgtggcgg cgtcgatcag
     3181 tcggcgaata ttccagacta tacgcttgtg atcggtaatg tgagggatta gccgttcttg
     3241 gacgtcaatc accagtagtg ccgtattgtc
//
LOCUS       DAOPTR010000396         3263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_23487_length_3263_cov_2.994389, whole genome shotgun sequence.
ACCESSION   DAOPTR010000396 DAOPTR010000000
VERSION     DAOPTR010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23487_length_3263_cov_2.994389"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..863
                     /gene="dapA"
                     /locus_tag="PKI05_08420"
     CDS             3..863
                     /gene="dapA"
                     /locus_tag="PKI05_08420"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOA52269.1"
                     /translation="MSVALITPFRDGQVDYARLEEQVEFQIQAGVTCLCPVGTTGESP
                     TLSHPEHREVISAVIKAANGRVKVMPGTGSNSTEEALELTRFAAQVGADAVLVVSPYY
                     NRPPQEGLYQHFKSLAQAVDIPICIYNIPGRTGRNIEPETFFRLAEFPNIAMVKEASG
                     SLDQVSQLTTQTDLTVLSGDDSLTLPILSVGGRGVISVIGNIVPQDMIAMIRAFEEGN
                     IPEAIRWHKKLFPLARDMLGLSTNPIPIKAAMRLLGRDSGELRLPLTPLSSDQENRLR
                     KVLADYGLLQ"
     gene            complement(906..1511)
                     /locus_tag="PKI05_08425"
     CDS             complement(906..1511)
                     /locus_tag="PKI05_08425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOA52270.1"
                     /translation="MFPHLRVLLVDHHDSFVYNLGRYFELLGCRIAIVRPTEVPESSN
                     ILRLFDLVVLSPGPCGPQNVVTSVELVHNLADQMPILGVCLGHQIIAEAFGGRIIRAA
                     EPVHGRATPVWHDGQHEFAGIPNPFPAGRYHSLIADRDSISEEVEISAWTDDGIPMAI
                     RHSRYPVVGWQFHPESVLTPLGLDLLRNLLLSWNQSLRFAR"
     gene            complement(1573..2484)
                     /locus_tag="PKI05_08430"
     CDS             complement(1573..2484)
                     /locus_tag="PKI05_08430"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOA52271.1"
                     /translation="MTTVLVVDDSPIDSQLVGSLLKKRFDWKIEYAESGTEALARMKQ
                     NVPDLVITDISMPEMDGLELVQHIRKMFPEVPVILTTAYGTDMLAIEALERGAASYVP
                     KSQLAARLAETADSVLRIAEQNRERERLFRCLEYIEFEYVLDNEAELIQPLVSVVQQT
                     LAGMEFADFAGRLQLGIALKEALLNALFHGNLEISKSEIEAVSDHLLEDSELSLVAQR
                     RQDPRYRDRKIYVLVRITKEEARFVVRDDGPGFDVHSVVRKKDESPELEAQRGLSLIQ
                     TFMDEVNFNEKGNEITLIKRREKLSKA"
BASE COUNT          736 a          911 c          873 g          743 t
ORIGIN      
        1 ggctgtcggt tgccttgatt acgccatttc gcgatgggca agtggactac gccagactcg
       61 aggagcaagt cgagtttcag attcaggccg gcgtgacgtg tctttgcccg gtcgggacaa
      121 ccggtgaatc acccacgctg tcacatcccg agcaccgcga ggttatctcg gctgtgatca
      181 aggcggcaaa tggccgggtc aaggtcatgc cggggacagg ctccaatagt accgaagaag
      241 ccctcgaact cacgcgcttc gccgctcagg tgggggccga cgccgttctg gtggtgtctc
      301 cctactacaa ccgacctccg caagagggcc tgtatcagca ctttaaatcg cttgcccagg
      361 cggttgacat tcccatttgt atttacaaca ttcccggtcg aactggacga aacatcgaac
      421 cggagacctt cttccgcttg gccgagtttc ccaatatcgc catggtcaaa gaggcaagcg
      481 gctctttgga tcaggtctca caattgacga cccagacgga cttgacggtc ctcagtggcg
      541 atgacagcct gaccctgccg atcctctccg tgggtggacg cggcgttatt tcggtgatcg
      601 ggaatatcgt gccgcaggat atgattgcga tgattcgggc ctttgaggaa ggcaacatcc
      661 cagaagccat ccgttggcac aaaaagctgt tccccttggc tcgtgacatg ctggggttgt
      721 ccacaaatcc catccctatc aaagcagcca tgcgactgct agggcgagac agtggagaat
      781 tacgtctgcc gttgacaccg ctttcttctg accaggagaa ccggctgcgc aaggtcctcg
      841 ccgattacgg tttgcttcaa tgagcaggtg agacattcgt ctccaaggga cacccttttt
      901 aagtgtcacc gcgcaaatct gagcgattga ttccacgaaa gtaacaggtt tcgtaaaaga
      961 tcaagtccca acggagtcag aaccgactcc gggtgaaatt gccaacccac caccgggtag
     1021 cggctgtgac gtatggccat gggtatgcca tcgtctgtcc atgccgagat ttccacttcc
     1081 tcgctgatcg aatctcgatc ggcgatcaac gagtgataac gtcccgccgg aaaagggttt
     1141 ggaattcccg caaattcgtg ttgtccgtcg tgccaaacgg gtgtcgccct gccatgcacg
     1201 ggttctgcgg ctcggataat cctcccgccg aaggcctcgg caatgatttg atgtcccaag
     1261 caaacaccga gaatcggcat ttgatctgcg agattgtgaa caagctccac ggaggtcacc
     1321 acgttctgag gaccacaggg acccggcgag aggacgacaa ggtcaaagag tcgcaaaatg
     1381 ttcgatgatt cgggtacttc cgtgggtcgc acaatcgcaa ttcggcagcc gagtaactca
     1441 aaatatcgcc ccaagttgta cacaaagcta tcgtgatgat cgacaagtag cacccgcaag
     1501 tgcgggaaca ttaggccatc ccctggcccc gtcacatttg acctgtaatg tggaaacacg
     1561 actcggaaat gattaggcct tggacagctt ctcgcgccgt ttgatcagag taatttcgtt
     1621 ccccttttca ttgaaattga cttcatccat gaacgtttga attaacgaca ggccgcgttg
     1681 tgcctccagc tcgggggatt catctttttt gcggacaacg ctgtgcacat cgaagccggg
     1741 cccatcgtcc cggacgacaa accgtgcctc ctcttttgtg atgcgaacca ggacatagat
     1801 cttgcggtca cgataccgag gatcctggcg ccgctgtgct accaaactca gttcgctgtc
     1861 ttccagcagg tgatccgaga cggcctcaat ctcactcttg ctgatttcca ggttgccgtg
     1921 aaacaacgcg ttcagcagcg cttccttgag ggcaatcccc aattgaaggc gaccggcaaa
     1981 atcggcaaat tccatcccag ccagggtctg ttgaaccacc gacacgaggg gctggattag
     2041 ctctgcttca ttgtccaaaa cgtactcgaa ctcaatgtat tccaaacaac ggaaaagacg
     2101 ctcccgctct cgattctgct ctgcaatccg gagaacggag tcggccgttt cggcaagccg
     2161 cgcggccaat tgggatttcg gaacgtaact ggctgcacca cgttccaggg cttcgattgc
     2221 caacatgtcc gtgccatagg ctgtggtcaa aatgacgggg acttcgggaa acattttccg
     2281 aatatgctgg accagttcca gaccgtccat ctcgggcatg ctgatatccg tgataacgag
     2341 atcgggcaca ttttgtttca tccgcgcgag cgcttctgtc cccgattcag catattcgat
     2401 cttccagtcg aatcgcttct tgagcagcga accaaccaat tgggaatcga tcggagagtc
     2461 gtccacaaca agcactgtgg tcataaaaac gcctcctatt aggtgcccta tgccgtctgt
     2521 atgttataac gaatcaacga ggcgtttact accatcacca gcgatttcac attttcccca
     2581 accatggtat cggtagggca ctctgcggct gaattctcga cctggggcgt gcggaggaac
     2641 acccggtgaa gaatcgtctc accactcaga ttaccacggt ttggattgtt tgaaagacac
     2701 tgtggctaac cggatttcgc ccgatctcga tccttgaaag aagccttgtg cagtcttaga
     2761 cttaaaagaa aggggtgccg cagcgctcag aacgtgttgg tgttttgcgt ggctcaggat
     2821 tttcgtcacg acgcgggttg ttgctacggc aagagttaaa agatgtccga taacgacgtg
     2881 gatgaccatc agcgttggaa ttcccgcccg gttgggggcc tttgcgtctg tctgttcttg
     2941 atggtccgct gaaccgacag ggcgcctccg gccgtgggtg ggttcgggga gtcaccggag
     3001 gcgcaacatg ggtcggttcg attctcaggt gcgcgcaact tcaattgcgt ctttctttcg
     3061 ccctatcgtt gcggccttta cgatcctctc ccggcacaaa aaccgactta ggccacgaca
     3121 cggaacattt ggcgacctcc cacccggccc ggcgaaatct ttttggagca tcccgcactc
     3181 aggtcccgta ttaggtgagg aaccctcgac aaaagaagat cgattgaccg cccaattggc
     3241 aagaagaagg tgagggctcg ggg
//
LOCUS       DAOPTR010000397         3262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_23493_length_3262_cov_3.327409, whole genome shotgun sequence.
ACCESSION   DAOPTR010000397 DAOPTR010000000
VERSION     DAOPTR010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23493_length_3262_cov_3.327409"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3110)
                     /locus_tag="PKI05_08435"
     CDS             complement(<1..3110)
                     /locus_tag="PKI05_08435"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HOA52272.1"
                     /translation="MGRLNSWRLTVYGQQVPGTPPDLVAVIPNEGSLIEEGAELLVAP
                     RELILRFNEGQQLDPRTLGAIQFVRAGGDDSFAEGNEVVVPIGYIGIGDKPNEVIVRF
                     AENLPDDKYHLVIKGTGANPLMNLDSPPLPFRYDPDLKTGRDLVMEFDINVGPQVLAV
                     VPQPVYRGDFEITAPAGNQITDGQTFRVGDGTYQVTFEFDTGNGVRAGNVPIVYASGD
                     SASLIAQKIGTAILNQGLNLTVLVNGAVVTLRGSQVSVDRQSSPLRVKSLYQERNVIE
                     VYFNNDKLDRNAAQDPRFYQLIFTRETLTPSDDLVVTPASVTYDAAKNLVRLVFAQDL
                     AHYGTGAARLRIGSAYQPIQTWNMSEGADAASSFYAATVIGGFGTGTVGQSVIISGSI
                     VTPIAYNLEWPGAIDEPGHRDLPNYDSILIEDHYLTGESSPDGSPQIPTIPFYFPKAS
                     WNLITEAQKQRAREIFDIYGYYLGIQFYETENSGLAIITGDLALMGLQSAPGGVAGVA
                     GMGVAVMDYAENWGASEFGGGWFQVAMHEIGHLLGYGHSYDLEPFVIMRSSEDAQSAV
                     GSFEPVFPGMADIIHGRHMFRPDNMDIDMYRFDLNERGLVDIEVLAERLNNSSLLDAT
                     ITLYREVQTLQKYVVIENGVPVEKEFTQTTYEVVARNDDFYSEDSAISVYLPAGRYYI
                     GVSASGNTQYDPTLDNSGIGGVTQGEYKLRVTYKPGGVDPNNPDTFREPNLANPSRLI
                     DSTGVYFDGDLDGIPGGDYNFWFTVQPESQTIFVDKLSGSSAPTGSIDNPFSTIAAAL
                     AVAQPGTIVRIVGNNFANDDPSNPATLQDNIPYEIGRRLVDNAPLSDGVRMDVPKGVT
                     VMIDAGAMIKLFRSNIMVGSIAEGIDRSGGALQVLGTPFHSVYFTSYYDESLGRDPEP
                     TNSTTPLSGDWGGIVFKNDLDYQFIRAYDPASGLPPRQVLEDQGIFLNYLSHADIRYG
                     GGEVIVNGVRSVYNPVHMVEARPTVVFSRITKSADAAMSADPNSFADTHYQSWDPNQP
                     YTVEYDR"
BASE COUNT          724 a          900 c          906 g          732 t
ORIGIN      
        1 cggtcatatt ccacggtgta gggttgattg ggatcccaac tttgataatg cgtgtcggca
       61 aagctgttgg gatcggccga catggcggca tcggcgctct ttgtgatacg gctgaagacc
      121 accgtcggcc gggcttcaac catgtgaacg ggattataga ccgagcgcac gccattgacg
      181 atgacttcgc caccgccata ccgtatgtcc gcatggctca ggtaattcaa gaaaatgcct
      241 tggtcttcga ggacctggcg tggcggcaag cccgaggccg ggtcataagc gcggatgaac
      301 tgataatcga gatcattctt gaagacgata ccgccccaat cgccggagag cggcgtcgta
      361 ctgttagtcg gctcaggatc ccgaccaagg ctttcgtcgt agtaggacgt gaagtacacc
      421 gagtggaacg gtgtgccgag gacttgcaaa gcaccgccgc tccggtcgat cccttcggca
      481 atgcttccca ccatgatgtt cgaacggaac agcttgatca tggccccggc gtcgatcata
      541 acggtgacgc ccttgggcac gtccatccgc accccgtccg atagcggcgc attatcgacc
      601 aggcggcggc cgatctcgta cgggatgttg tcctgaaggg tggctgggtt gctaggatcg
      661 tcgttggcaa aattgttgcc cacaatccgc acgatagtgc cgggctgagc gacggccaag
      721 gcggctgcga tggtgctgaa aggattgtca atcgaaccag tgggggctga agaccctgag
      781 agcttatcga caaagattgt ctgcgactct ggctgcaccg tgaaccagaa gttgtaatca
      841 ccaccgggga tgccatccaa gtcaccatcg aaatagacac cggtcgaatc gatcagccgt
      901 gacgggttgg cgaggttcgg ttcacggaac gtatcgggat tgttcggatc tacaccgcct
      961 ggtttatagg tcacgcggag tttgtattca ccttgtgtta cccccccaat gcccgagttg
     1021 tccagtgtcg ggtcgtactg tgtgtttcca ctggcactga cgccaatgta ataccgccct
     1081 gctgggagat aaacactgat agccgaatcc tcgctgtaga agtcgtcatt ccgagccacg
     1141 acctcatacg tggtctgcgt gaattctttc tccactggga cgccgttttc tatcaccacg
     1201 tacttctgca gcgtctggac ttcgcgataa agagtgatcg tcgcgtcgag aaggctcgag
     1261 ttgttaagtc gctctgccag gacctcgatg tccacgagac cgcgctcgtt caggtcgaac
     1321 cggtacatgt cgatgtccat gttgtccggc cggaacatgt ggcgcccgtg gatgatgtcg
     1381 gccatgccgg ggaagaccgg ctcaaagctg ccgacagcgc tttgggcgtc ctcagagctg
     1441 cgcatgatga caaagggctc gaggtcgtaa ctgtgaccgt aaccgaggag gtggccaatt
     1501 tcgtgcatgg cgacctggaa ccatccaccg ccaaattcgc tggcgcccca gttttcggcg
     1561 tagtccatga cggcaacgcc cattccagcc actccggcaa cgccacccgg agccgattga
     1621 agccccatca aggccaagtc gcctgtaatg atggccaacc cagagttctc tgtttcatag
     1681 aactgaatgc ccaaatagta tccgtaaatg tcaaagatct ctcgggcgcg ttgcttttgg
     1741 gcctccgtaa tgaggttcca gcttgctttc gggaagtaaa aaggtattgt cggaatctgc
     1801 ggggaaccgt ccgggctaga ttctcccgtc agatagtggt cttcgataag gatgctgtca
     1861 taattgggaa ggtctcgatg ccctggctcg tcaatggcac caggccactc aaggttgtaa
     1921 gcaatgggtg ttacgatgga cccagagatg ataacgctct gtccgaccgt tccggtgcca
     1981 aacccgccaa tgaccgtcgc agcgtaaaaa ctagatgcgg catcggcccc ttctgacata
     2041 ttccatgttt ggatgggttg atacgcgctg ccaatgcgga ggcgtgctgc gcccgttccg
     2101 taatgcgcaa gatcttgcgc aaacaccagc cggacaagat tcttcgcggc atcgtaagtg
     2161 accgacgctg gtgtaacgac aagatcgtcg cttggagtga gtgtttctcg agtgaagatc
     2221 aactgataga agcgcggatc ttgagcagca tttcgatcaa gcttgtcgtt gttgaaatag
     2281 acctcgatga cattccgctc ctgatacagg ctcttgaccc gcaatggact gctctgacga
     2341 tcgacagaga cctgacttcc acgtaatgtg acaactgcgc cgttgaccaa aaccgtcagg
     2401 tttagccctt gatttaaaat agcggttcca atcttctgcg caatgagcga tgcggaatca
     2461 ccgcttgcat atacgatagg tacattgccc gcgcgtacac cattccccgt gtcgaattca
     2521 aaggtgacct ggtaggttcc atcgcccaca cggaacgttt gaccatcggt gatctggttt
     2581 cccgcaggcg cggtaatctc gaagtcacca cggtaaacgg gttgcggaac gacggccaac
     2641 acttgaggtc cgacattgat gtcgaactcc atcactaggt cacgacctgt cttaaggtcg
     2701 gggtcataac ggaatggcaa aggcggcgaa tcgagattca ttaacgggtt ggcacccgtc
     2761 cctttgatca caagatggta cttgtcgtcc ggcaggtttt cggcgaaacg gacaatgact
     2821 tcattcggct tatcaccaat cccgatatag ccgatgggaa cgacaacctc atttccttcg
     2881 gcaaaggaat catcaccacc agctctcacg aactgaatgg cgccaagggt ccgcgggtca
     2941 agttgctgtc cctcgttaaa ccgcagaatc aactcgcgag gagcaacgag tagttcagct
     3001 ccttcctcga tcaggctccc ttcgttgggg atcactgcca cgagatccgg cggcgttcca
     3061 ggaacctgtt gtccgtaaac ggtcaagcgc caggaattga gccttcccac aacgccaccc
     3121 actcggtccc gaacgatcag tgtccattcg cctcccgacg tttctcccca gttacggttt
     3181 gttgtaaacg tccactgaac ccctgtatcg aatggattgt gaatgtgtgg agtcgcaagg
     3241 actgattgtg taccggaagg cg
//
LOCUS       DAOPTR010000398         3258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_23536_length_3258_cov_2.700905, whole genome shotgun sequence.
ACCESSION   DAOPTR010000398 DAOPTR010000000
VERSION     DAOPTR010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23536_length_3258_cov_2.700905"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2112)
                     /locus_tag="PKI05_08440"
     CDS             complement(<1..2112)
                     /locus_tag="PKI05_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52273.1"
                     /translation="MTIRRNAPSRKGVVLLLVLALLSVFGLIVLAYVVLTGHTRRGAE
                     AAKRFQEQYDPPEQTLMQVAMEVIRGSNNPRSSIWCHSLLEDIYGDRFQEGSISGANS
                     LNATAGGQLWVFPANFSNAEQWVGNVLTFVSIIPPDVAAKVRPLAGISTRIVDYDAVT
                     NLLHVVAPQSPSVTDVQEALKSGARISYIINGRPFSGTGVGYGGQDSLNLFASWPDRQ
                     DPSRLVSFPIALLVNGSSRKFQQYVGQPLAYSYLGPDNTPGTDDDWPGRDGIVGVNPG
                     PDGLLGTTDDLPPDDSYGPDGIPMTPDDGTVFFWPLSANEDYDAPDYQNMFLAGLSYD
                     SNNNRVFVSEPSFHRPTLLKYLFNKGVFNNAPDLKRLFCLRPQRLFHPNFDGSNPDYR
                     DIPDDSNPTPWDVDNDGDGVPDSIWVDVGLPVRQLPDGRRYKPLAAILCVDLDGRLNL
                     NAHGSPRSPVQTSLPREIVAVPAAPAKLGIGPAEIEVPWVVASRYASDASDSVPGKPG
                     LDPLADWQMAQFPREYLLFPVDLFGATGLALDCLGQPLFFPAFTNPSWQKADNPYELD
                     LLGTAAYCASVDSPFTPAELERLLRPFDADSRDLPKRSSLNLPLHDRFSVTTHSFDVP
                     SVPRTLPPRILKLFLEQRAKDIGLVQQQVAVYPELQEFLAFLDRGALNGEDWLSFIQV
                     HVYLEQRMFNLAPISRDDINTI"
     gene            complement(2240..>3258)
                     /locus_tag="PKI05_08445"
     CDS             complement(2240..>3258)
                     /locus_tag="PKI05_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52274.1"
                     /translation="VASVGRNVLADVRTREWLDATRWMNVDGSPVVPHRDAPLPIGEA
                     FCIDPLYIAAVINQAGGLPAPELGRFPYDPTPTVLSMRRVSVNVAVNPTQPQLVLSNR
                     RFWERLCVAEDDLVFDSTALSRPRLVFRTDAGKIMAVPTLPGETPAGNPLYPDYQGMY
                     SWMVTVVPQVPGVPSVSQSYLVSAVIFRNRNLLWPVDPAADMPGERVAIANFLGGADL
                     RLSVPNSAVGTRDPDEYLRIPEGQYLLLAGRVLVNVNPPQFQNVFLWYRVIAASELSQ
                     NGSFYQRDLTVAGPDWNPGWCLDIDGDGNSREAWAILCTNVYGVYTEVMGAEALSRWS
                     WPVR"
BASE COUNT          807 a          902 c          891 g          658 t
ORIGIN      
        1 aattgtatta atatcgtccc gcgatatggg cgcgagatta aacatgcgct gctcgaggta
       61 aacgtgcacc tgaatgaagg aaagccaatc ttctccgttg agtgccccgc ggtcgagaaa
      121 tgcaagaaac tcctgaagct caggataaac cgctacttgt tgttgtacta acccaatatc
      181 cttggctctt tgttccaaaa atagtttcaa aatccgcgga ggcaatgtac gagggacgga
      241 tggaacatcg aacgaatgcg tcgtcactga aaaacgatcg tgcaagggta agtttagtga
      301 tgaccgcttc ggaagatccc gagaatcggc gtcaaaaggt cgcagcagtc gctccaactc
      361 tgctggtgtg aaaggcgaat caacacttgc gcagtaggcc gcagtaccca gaaggtctaa
      421 ctcataagga ttgtcagcct tttgccagct cgggttagta aacgcaggga agaaaagtgg
      481 ctgccccaga cagtccaagg ctagccctgt ggcgccaaac aaatcgacgg gaaacaacaa
      541 atactctcga ggaaactgtg ccatttgcca atcagcaagt ggatctaggc caggttttcc
      601 cggcacgctg tcagaagcat cagaagcata acgtgacgca acaacccaag gaacctcaat
      661 ttctgccggg ccaataccaa gctttgccgg agcggcgggt accgcgacga tctcgcgcgg
      721 aagtgaagtc tgaactggag aacggggact accgtgagcg ttgaggttaa gacgcccatc
      781 caagtccacg cagaggatgg ccgccagtgg cttgtagcga cgcccatcgg gcagttgtcg
      841 gaccggtaaa cccacgtcca cccagatgct atccggaacg ccatcaccgt cattatcgac
      901 gtcccacgga gtgggattgc tatcatcagg gatgtcgcgg tagtctgggt tgctgccatc
      961 gaaattcggg tgaaaaagtc tttgcgggcg caagcagaat agacgcttaa ggtctggtgc
     1021 gttattaaac actcccttgt tgaacaaata cttcaggagt gtcgggcgat ggaatgacgg
     1081 ctcagacacg aacacccggt tgttgtttga atcataactc agcccagcca aaaacatatt
     1141 ctggtaatcc ggcgcgtcat agtcttcgtt ggccgataat ggccaaaaga acacggtacc
     1201 gtcgtcgggg gtcatgggaa ttccgtctgg accgtaacta tcgtcggggg gcaaatcgtc
     1261 agttgtgcca agaagtccat ctggccccgg gttaacacca actatgccgt ccctgccagg
     1321 ccagtcatcg tctgttcccg gggtattgtc tggtcccaaa taagaataag caagtggctg
     1381 accgacgtat tgctgaaatt tgcgacttga cccgttcact aataacgcga tcggaaatga
     1441 aacgagtctg gatgggtctt ggcgatcggg ccagctcgcg aaaagattaa ggctgtcttg
     1501 gccaccgtag cctacgccag ttccagaaaa tgggcgacca ttgatgatgt aactgattcg
     1561 ggctccactc tttaaggcct cttgtacgtc agtaacggac ggtgattgtg gtgccacgac
     1621 gtgaagcaaa ttggttaccg catcatagtc cacaattcgt gtgctgattc cggcgagcgg
     1681 tcggactttg gcggcaacat cgggggggat tatacttaca aacgtgagga cgttgcccac
     1741 ccactgttcc gcattactaa aattagcagg gaaaacccag agttgtccgc cagcggtggc
     1801 gttaagggaa ttcgcgccgc taattgatcc ttcctgaaac cgatccccgt agatatcctc
     1861 cagcagacta tggcaccaaa tggacgagcg aggattgttc gaccctcgga tcacctccat
     1921 ggcgacctgc atgagagtct gctcaggcgg gtcatactgc tcctggaagc gtttggccgc
     1981 ctcggctccc cgccgcgtat gaccggtcaa aaccacatag gccaagacga tcaagccaaa
     2041 gaccgagagc aacgctagaa ccagcagaag tacaacacct ttccgcgatg gagcgttcct
     2101 tcggattgtc atggttcacc ctactacatc tcttgaggcc gcggtcttgc ccaacgggcc
     2161 gcggcaagga ttgtcttcgt caggtcgagc cacttctgcc tcaaatcatc tttagatcta
     2221 aacgtctgtc gcgcggagat taacgaacgg gccaggacca gcgggacagg gcctcggcgc
     2281 ccatcacctc ggtataaacc ccataaacgt tcgtacacaa aatggcccac gcctcacggc
     2341 tgttgccatc gccatcgata tctaggcacc agccaggatt ccaatccggg cctgccactg
     2401 tcaggtccct ttggtaaaaa ctgccgtttt gcgacagttc gctcgccgca atgacacggt
     2461 accacaaaaa gacgttctgg aactgcggcg gattgacatt gaccagtacc cgtccggcca
     2521 aaaggagata ctgtccctcg gggatgcgga ggtactcgtc gggatcacga gttcccaccg
     2581 cggagttggg aaccgacaat cgcaggtcag caccccctag aaaattggca atggcaacac
     2641 gttcccccgg catgtcggcc gcgggatcaa ctggccaaag cagatttcga ttgcgaaaga
     2701 tcactgccga aaccaaatag ctttggctaa cgctgggaac gccgggtact tgagggacca
     2761 ccgttaccat ccaggagtac atcccctgat agtcgggata aagcggattc ccggcgggcg
     2821 tctcacccgg cagcgtcgga actgccataa tctttccagc atctgtccga aagacaagcc
     2881 ttggccggga taacgcggtg ctatcgaaga ctagatcgtc ttccgcaaca cacagccgtt
     2941 cccaaaaccg ccgattactc aggaccaact ggggctgagt gggattgacc gcgacattca
     3001 ccgagactcg tcgcatactc aagaccgttg gcgtcggatc ataggggaaa cgccccaatt
     3061 caggcgccgg caacccaccg gcctgattga tcacggcggc gatgtacagc gggtcgatgc
     3121 agaaggcctc gccaatgggc agcggtgcat cgcggtgcgg caccacaggt gaaccatcga
     3181 cgttcatcca acgggtggcg tcaagccatt cacgggtgcg gacgtcggcc aggacgttgc
     3241 ggccaacgct ggccacgc
//
LOCUS       DAOPTR010000399         3251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_23603_length_3251_cov_3.194931, whole genome shotgun sequence.
ACCESSION   DAOPTR010000399 DAOPTR010000000
VERSION     DAOPTR010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23603_length_3251_cov_3.194931"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="PKI05_08450"
     CDS             <1..788
                     /locus_tag="PKI05_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52275.1"
                     /translation="GLAGEVTFLQAGPEVSLSQAINMGIEAANTPLIHVLPAGSCVHA
                     GWAESAMPHFQDSRIGAVIPVTLELQHPDRILAAGMGYDSAGRLIYLQRGISLRDTQL
                     CHRTQVLPHFSGAIFRAASLHALHGADESVGERWVFADLGLRLLRAGAVTVLEPHCWV
                     SSDPATEPASLGYAAARDAEAFFWRWCDRRSTTLARHLALITRETLAAVPERQVWPSV
                     AGRIAGFTQRRHNGRPRPKAFSQDTKPTKSVASDLGIPMARAG"
     gene            complement(809..1873)
                     /gene="fliM"
                     /locus_tag="PKI05_08455"
     CDS             complement(809..1873)
                     /gene="fliM"
                     /locus_tag="PKI05_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HOA52276.1"
                     /translation="MSSDEVLTQAEIESLLASVGKGKSAPSPTPSPQPAAPSPKTPAA
                     PPSAPPRKSREKIIPYDFKRPERVGKEQMRSLQTLHEGFSRNFAASLSAMLRSMVEVK
                     LTSVDQLTYSEFVFSLDNPTCFNLIKAEPLEGHIILDLNPTILYPMIDRLLGGGREPT
                     PITRRPLSEIERRLVGRITNLFLDELKKAWENILPLQMEVVRVESNPQLVQIVPPNEV
                     VVLVSFELALGEIRGMANLCIPYNTIERISNKLSANSWASYGKRQTTPDAAERIGRLL
                     RTSTVQVVVELAKTHITTEDLIGLRVGDIITTRKDIHTPLVVKVEGIPKFLARPGIFK
                     GHKAICIEEIVTDPTQALTD"
     gene            complement(2175..>3251)
                     /locus_tag="PKI05_08460"
     CDS             complement(2175..>3251)
                     /locus_tag="PKI05_08460"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOA52277.1"
                     /translation="FGLTEAQIDRLKLTGPRIEAMAKALEEVALLPEPIGEVIWSSVR
                     PNGLLVQKVRVPLGVIFFIYESRPNVTADAAALCVKSGNAVILRGGKEAANSNRAIVD
                     IMLEAGADVGLPVDAIQLVATPDREAVGHLLNLPEYIDLTIPRGGESLIRRVTAEAKM
                     PVMKHFAGNCHVYIDASADPDMAERIVINSKCQRMGVCNAAESLVFHRAVADTLLPRI
                     LQALSERGIEIRGDEETCARFPAAKPAIEDDYFAEYLGPIISVKVVDDLDAAIEHINH
                     YSSKHTEAIVTRDLLAAQEFAQRIDSSAVMINASTRFNDGGEFGLGAEIGISTDKFHA
                     RGPCGINELTSYKYVVIGNGQVRE"
BASE COUNT          631 a         1012 c          925 g          683 t
ORIGIN      
        1 acggattggc gggggaagta acctttctcc aggcgggacc ggaagtcagt ctctcgcagg
       61 caatcaacat ggggattgag gcggctaata ccccgttgat ccatgtcctg cctgccggca
      121 gttgtgttca tgccggctgg gcggagtcag ccatgcccca cttccaagat tcccgaattg
      181 gggccgtcat ccccgtaaca ttggagctgc agcatcccga tcgcatcttg gcggcgggaa
      241 tgggttacga cagcgccggc cgattgattt atctccaacg cggcatctcc ctgcgtgata
      301 ctcaattgtg tcaccggacc caagtgctcc cgcatttctc gggggcgatt ttccgagcgg
      361 ccagcctcca cgcactccac ggggccgacg agtccgtcgg ggagcgttgg gtgtttgccg
      421 atctcgggtt gcggctgttg cgggctgggg cggtcacggt gctggaaccg cattgctggg
      481 tatccagtga cccggcgacg gaaccagcct cgctgggata tgcggcagct cgagacgcag
      541 aggccttctt ctggcgctgg tgcgaccgcc gcagcacgac gctcgcgcgg cacctggcgc
      601 ttatcacccg cgagaccttg gccgccgtcc cggagcgtca ggtgtggccc tccgtggcgg
      661 gtcgcatcgc cggattcacg cagcgccgcc acaatggtcg gccccgccca aaggccttct
      721 cacaggatac gaagccgacc aagtccgtag cctcggacct gggaatcccc atggcccgcg
      781 cgggttgaat tcgccccttc gctgagactc agtcggtaag cgcctgggtg ggatcggtga
      841 cgatctcctc gatgcaaatc gccttgtgcc ccttgaaaat ccccggccga gccaaaaact
      901 tgggaatccc ttccacctta acgaccaacg gcgtgtgaat atccttgcgc gtggtgatga
      961 tgtcgcccac gcggagacca atcaggtcct cggtggtgat gtgcgtcttg gccagctcca
     1021 ccaccacttg aacggtcgag gtccgcaaaa gtcgcccaat ccgttcggcg gcatcaggcg
     1081 tggtctgtcg cttgccatag gaggcccaac tgttggccga aagcttgttg gaaatccgtt
     1141 caatggtgtt gtaggggata cacaaattgg ccatgccacg gatttcccct agggccaatt
     1201 caaagctgac gagcaccaca acctcgttgg gcgggacaat ttggacaagc tgaggattac
     1261 tctcgacgcg gacgacctcc atttgcagcg gcaggatgtt ttcccatgcc tttttcaatt
     1321 catcgagaaa caggtttgtg atccgcccca ccagtcgtcg ttcgatctcg gaaagcggcc
     1381 gccgcgtgat cggggtcggc tctcggccac cgcccagcag ccgatcgatc atcgggtaaa
     1441 ggatggtggg attcaaatcc aggatgatat ggccttccag cggctcggcc ttaatgaggt
     1501 tgaagcacgt gggattgtcc aaactgaaca cgaactcgct gtaggtcaat tgatccacgc
     1561 tagtcagctt cacctcgacc atggatcgga gcatggccga gaggctggcc gcgaagtttc
     1621 ggctgaaccc ctcgtgcagc gtctggagcg agcgcatttg ctcttttccc acgcgctccg
     1681 gacgcttgaa atcataggga atgatcttct cccgactttt gcgcggcggt gcagagggcg
     1741 gcgccgccgg ggtcttagga ctgggggccg ctggttgcgg cgacggcgtg ggagaaggtg
     1801 cactcttgcc cttgcctacc gatgcgagca gactttcaat ttccgcctgt gtcagcactt
     1861 cgtcggatga catgcgatcc tccgcaactc aggcaaccgt gccactgtct ccgaactcac
     1921 tcatccccgg tgcatcctcc caaggatatt ttatttggag cctatcccat aatttccccc
     1981 tcacccgttc ggcgggcgag gggtctgggg tgaggctggc tcgaaccgct gcccggtttt
     2041 cccgatcgac tccgaccctc gcttgccctc accccagccc tctcccgggg ggagaggggg
     2101 acttttcgaa acagactccc atccgttcgc tcggcgagac atcgccagcg tgggcaaaca
     2161 ccgattactg cccctcattc ccgaacttga ccgttaccta tcaccacgta cttgtagcta
     2221 gtcaactcgt tgattccgca tggaccacgg gcgtgaaact tatctgtact gattccgatc
     2281 tccgcgccaa ggccaaactc accgccatca ttgaatcgcg tgctggcatt gatcatgacg
     2341 gcggaactgt caatccgctg cgcgaattct tgggcggcca ggaggtcccg cgtcacaatg
     2401 gcctccgtgt gcttggaact gtaatggttg atgtgctcaa tggcggcatc gagatcgtca
     2461 accaccttta ccgatatgat gggacccagg tattccgcaa aatagtcgtc ctcgatggcg
     2521 ggtttggcgg caggaaatcg ggcacaggtc tcctcatcgc cacggatttc gatcccccgt
     2581 tcgctcaggg cctgcaagat ccgcggtagc aacgtgtcgg ccactgcccg atgaaagacc
     2641 aacgattccg cggcattgca cacacccatc cgctggcatt tggaattgat gacaattcgc
     2701 tccgccatgt ccggatcggc cgaggcatca atatagacgt ggcaattccc cgcgaaatgc
     2761 ttcatgacgg gcatcttggc ctcggcagtc acgcgacgga tcaggctttc tccaccgcgc
     2821 ggaatggtca aatcgatgta ctccgggaga ttcaacaggt gccccaccgc ttcccgatcg
     2881 ggagttgcca caagttgaat cgcatcgacg ggcaaaccta cgtcggcccc ggcttcaagc
     2941 atgatatcga caatcgcccg gtttgagttg gcagcttctt ttcccccgcg gagaatcacg
     3001 gcgttgccac tcttcacaca aagggccgcc gcatcggccg tgacgttcgg ccgcgattca
     3061 taaatgaaaa agattacccc caacggtacg cgaacttttt gaaccaacaa accgttggga
     3121 cggaccgacg accaaatcac ctcgccaatt ggctctggga ggagggccac ttcctccagg
     3181 gccttagcca tcgcctcgat ccgtggcccc gtcagtttca gccgatcaat ctgagcctcc
     3241 gtcaaaccaa a
//
LOCUS       DAOPTR010000400         3240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_23699_length_3240_cov_2.976766, whole genome shotgun sequence.
ACCESSION   DAOPTR010000400 DAOPTR010000000
VERSION     DAOPTR010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23699_length_3240_cov_2.976766"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PKI05_08465"
     CDS             complement(<1..259)
                     /locus_tag="PKI05_08465"
                     /inference="COORDINATES: protein motif:HMM:NF021882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52278.1"
                     /translation="MIRNPEETIAAIATPKGFGARLIIRISGPETWQAVHRVITICDN
                     DLPVVPAEPVIRAASLRLPQLQGTVPCELYYWPPGRSYTGQA"
     gene            complement(269..2788)
                     /locus_tag="PKI05_08470"
     CDS             complement(269..2788)
                     /locus_tag="PKI05_08470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family insertase periplasmic-domain
                     containing protein"
                     /protein_id="HOA52279.1"
                     /translation="MLGQDNSRIWARYFAFLAISMAIIVVNIYLSSLFSPQPNKVQPP
                     PAEKVVADNASGEIKGTQPSSEPKTEEGPKQPSASEEETAVSQSTASRQEEKKEPDES
                     PRSLPKPPTRYLTLGSLDPAGPYRMLITLSNRGASVVSIEMSSLRYCDTEQPGGYLGA
                     IAIDPAAGEDGVRVDVVGPGTPAAEAGLLPGDVITEIENQRVYDLRSMEAILSRCRPN
                     QSVKIRVRRGEKEMVLDARLRRPPLRLIRREAGAPFSFEMALALANKTRLADLRAPFE
                     ERMAEVKDPMEKELLYLQWLHAEIPELGMRSENWHILADTSDPRRPETWGNTEIDSIP
                     QPRESVRFGIYHERLGLVVLKTYTLTKVPADKQTDPDNPSYHFDLTIDVINVGKDAQE
                     IAYELDGPNGLPTEGAWYARKMSNSWSAVGLRDVAVRMGKGAVQLVACPAIVKDKWGV
                     PWKDASETITALGVDAQYFSVIMLPKRSRLDEPWFDEARPIRVGPVEPDYQFLTNVSC
                     RVHSIPTVLQPGGQLTHEYTIFAGPKRQKLLATYGLHGLIQYGWFWFVADPMTKLLHL
                     FHNYLVFNYGLAIILLTVIVRLAMFPISRKQALNVQKMQELQPEIKKIQEKYKDLQER
                     NRATQELFRQHNYNPFSGCLVLFIQLPIFIGLYKALAIDVELRQAPLISESIRWCSNL
                     AAPDMLFDWSFLWPEWFNRGQGMFALGPYFNILPILTIAIFLWQQKKMMPPPADEQAA
                     MQQKIMNFMMIFMGILFYKVPSGLCIYFIASSLWGMAERQILLKTTQKCPDRGADQRK
                     RRKQWSRVKIQLPQWLVQLLKAADKNQAEKLARRQRSTITK"
     gene            complement(3055..>3240)
                     /locus_tag="PKI05_08475"
     CDS             complement(3055..>3240)
                     /locus_tag="PKI05_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52280.1"
                     /translation="SHLVMNGHALWWPDHGIGLLVQIWAVNRAGSLDRGLMFPQARRL
                     FVPLFLSKGTPCREHTG"
BASE COUNT          735 a          877 c          864 g          764 t
ORIGIN      
        1 cggcctggcc ggtgtagctt cggcccggtg gccagtagta gagctcacac ggcactgttc
       61 cctggagttg gggcaaacgc aaagacgcgg cccgaataac cggctctgcg ggcaccaccg
      121 ggaggtcatt atcacaaatc gtgatgaccc ggtggacggc ttgccaggtc tcgggacctg
      181 aaattcttat gatcagccgt gcaccgaatc ccttcggggt tgcaatagct gcaatcgtct
      241 cctcgggatt gcggatcata gccgaggctc atttggtgat tgtggagcgc tgccgtcgcg
      301 ctagcttttc cgcctgattc ttatcggctg ccttaagcaa ttggaccagc cattgtggta
      361 gctggatttt gacgcggctc cattgcttcc gtcgctttcg ttgatcagca ccacgatctg
      421 gacacttttg cgtcgttttg agaaggattt gccgctctgc cattccccaa aggctggagg
      481 cgatgaaata gatacagaga ccactgggta ccttatagaa aagaatcccc ataaagatca
      541 tcatgaaatt catgattttc tgctgcatag cagcttgttc atcggcgggc gggggcatca
      601 tcttcttttg ctgccaaaga aaaatggcga tagtcaagat cggtaagatg ttgaaatatg
      661 gcccaagggc aaacatcccc tgaccgcgat tgaaccattc cggccacagg aaactccaat
      721 cgaacagcat gtcaggcgcc gccaaattcg agcaccagcg aatcgactca gaaatcagtg
      781 gtgcctgacg gagttcgacg tcaatggcca gtgccttata cagtccaatg aaaataggaa
      841 gctggataaa aaggaccagg cacccggaga acgggttgta gttgtgttgt cggaaaagct
      901 cttgtgtcgc tcggttccgt tcctgaaggt ccttgtattt ctcctggatt ttcttgatct
      961 ccggttgaag ttcctgcatt ttctgaacgt taagggcctg ttttcggctg atcgggaaca
     1021 tcgccagcct gacgatcacc gtcagtaaaa taatggccag cccatagttg aagaccaagt
     1081 aattgtggaa cagatgcagc aatttcgtca tagggtctgc cacgaaccag aaccagccat
     1141 actggattaa tccgtgaagg ccgtacgtgg caagcaattt ctgtcgtttc ggtcccgcga
     1201 atatggtata ttcgtgggtc aattggccac ccggctggag aaccgtaggt attgaatgaa
     1261 cacgacacga gacgttagtt aagaactgat aatcaggttc aaccgggccc actcgaatag
     1321 gacgggcctc atcgaaccag ggttcgtcaa gacgtgacct cttgggaagc atgatcaccg
     1381 agaaatactg ggcatctacg cccaaggcgg tgattgtttc gctcgcatct ttccaaggga
     1441 cgccccattt gtccttaaca atagccggac atgcgaccaa ttgaacggca cctttaccca
     1501 ttcgaaccgc cacatcccgt aggccaacgg cgctccaact gttgctcatt ttgcgggcat
     1561 accaagctcc ctccgtgggc agaccgttag gcccgtccaa ttcatatgct atttcttgag
     1621 catctttccc cacgttaatg acgtcgattg tcaggtcgaa atggtagctt gggttatcgg
     1681 ggtcggtttg cttgtccgcc gggacttttg tcaatgtgta tgtttttaag acaactagcc
     1741 ccagtcgctc atggtaaatg ccaaatcgca cagattctcg cggttgaggg atcgagtcaa
     1801 tttctgtgtt accccatgtt tcagggcgac gtggatcact cgtgtcggcg aggatatgcc
     1861 agttttccga acgcattccc agctcgggaa tctcggcatg cagccactgc aaataaagga
     1921 gctccttttc catggggtct ttgacttccg ccatgcgttc ttcaaaaggc gcacgcaggt
     1981 cggcaagtcg cgtcttgttt gctagggcga gggccatctc aaaggagaaa ggcgcaccgg
     2041 cctcgcgtcg aatcagtctc agtggtggac gccgcaacct agcatcgagg accatctctt
     2101 tttcgccgcg ccggacgcgg atttttacgg actgattggg ccggcagcgt gaaagaatgg
     2161 cctccatgct ccgcaagtcg tacaccctct gattttcgat ttcggtgatc acgtcgccag
     2221 gtagcaatcc agcctcggca gcgggggttc ccggaccgac cacatccact cgaacgccgt
     2281 cctcaccagc tgcaggatcg atggcaatgg ccccaagata accaccgggc tgctcggtat
     2341 cgcagtatcg gaggctactc atctcgatgg agaccacaga cgctccacgg ttcgacagtg
     2401 tgatcaacat ccgatatggt cccgccggat cgagcgaacc cagcgtgagg taccgagtag
     2461 gcggtttggg gagtgaacga ggcgattcgt cgggctcttt cttttcttct tgccgagagg
     2521 ccgtggattg cgaaacagcc gtttcttctt ccgaggcact cggttgcttg ggaccctcct
     2581 cggttttggg ctctgagctc ggttgagtgc cttttatttc gcccgacgca ttgtccgcga
     2641 cgaccttctc cgccgggggc ggctgtacct tgttcggttg gggcgagaag agggaagaca
     2701 gataaatatt gacgacgata atcgccatag agatggccaa gaatgcgaaa tatctcgccc
     2761 aaattcgact attatcctgt ccaagcatcg cagcctttca aatctcaaaa aacgagttgc
     2821 attcaaactt tgataggtca gaccgggtaa ccgctatccg aggaatggcc ctccccagtg
     2881 gcaaatcata ccaccccaag gtggcaattt ctcaggaggc ggtccaccac gtttgccggt
     2941 aattgttgcc ctattttccc cactgtggcc aataagtgtt attgtgaatg attcccgaaa
     3001 ttacgcaagg tccaaagagg gacggcggcg gggactgtac cagcggcaca ccaatcatcc
     3061 ggtgtgttcc cggcaaggcg tccctttact cagaaatagc ggcacgaaca gccgtcgggc
     3121 ttgtgggaac atcaggcccc gatctagact gccggctcga tttaccgccc aaatctggac
     3181 cagcaagcca atcccatgat ctggccacca cagcgcgtgg ccattcataa ccaaatgact
//
LOCUS       DAOPTR010000401         3233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_23771_length_3233_cov_2.744179, whole genome shotgun sequence.
ACCESSION   DAOPTR010000401 DAOPTR010000000
VERSION     DAOPTR010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23771_length_3233_cov_2.744179"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..1348)
                     /locus_tag="PKI05_08480"
     CDS             complement(266..1348)
                     /locus_tag="PKI05_08480"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOA52281.1"
                     /translation="MKGSNRMVRRAFTLVELLVVIAIIGILIALLLPAVQAAREAARR
                     SQCTNNLKQIGLALHNFHDVYGVFPVGQPDDDNDNYSWSAYILPFMEQKSMYDTLVGG
                     GAALVYIPGGDNLKVHGAIREIPGVSPALPSGHPVRNTDSYNWWTQVKSDHGNPNHAN
                     NVGAKSVINSYICPSDVLPKQDNNGYGKSNYCCSLGSDEPWVNQYMSGGLSWSEPSAS
                     LQNGMFRLAQTNDYHFVTTFSEIIDGTSNVIAVGEVSESPNISRTQTDRMFPLWAGGN
                     NDWAGQWRISSWARVAGPYAVINCKDTMPIGSVTLADYAFGSKHPGGANFLLGDGSVR
                     FLSETIDGTLYGRLADIRDGNPVTMP"
     gene            complement(1794..3125)
                     /locus_tag="PKI05_08485"
     CDS             complement(1794..3125)
                     /locus_tag="PKI05_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HOA52282.1"
                     /translation="MLGHLSAWCVRWIASVGCAIAVFISSMTSADDWPQWRGPNRDGI
                     WRETGITRQFSGPEVKLRWRTKIAGGYSGPTVADGRVFVTDRLTEPEEVERVHAFDWQ
                     TGQCLWTFSYPCVYRNVTYRTGPRASVTVHEGLAYALGTMGDLHCLDAETGTVVWKKR
                     AGQDWEVRVPTWGVAAAPLVEGDLLIVQIGLEDGCVVALDRKTGELRWKALPDQASYS
                     APITITQAGRRVVVCWTGERLAGLDALTGKLLWDVPFPHRRWIDGIITPVWDSSTNRL
                     FISCFDCGSLMLHVDSNDLHVQKIWRRQGQNEINTDSLHILMGNPVIKENWIFGVDSY
                     GQFRCLDARSGDRIWEDLTLTSQLRWGTLHMVQQADVVWMFNDRGELMITELTPQGVH
                     VISKARLLAPTRGQLSRGPGVTWSHPAFAHRHVFNRNDEELVCADLSEGAQ"
BASE COUNT          773 a          938 c          831 g          691 t
ORIGIN      
        1 gctgccccgc tcttggaaca accgattgcc gtagcaagca ctcccgccac agccagaacg
       61 agaaacacac gcaaacattg cattgctaga ctcataggga cgcctcctga aatcgacatc
      121 acatcccgcg cacgaacgcc gccacagaca ccaaattatt atgatacaag gcggctaccg
      181 ttgccaacga cagccgcctt attaaccacc cgtgatcgcc aaacttagga ttacggtttt
      241 ggcgacttgt aatcccctat gcaaactacg gcatggttac gggattgcca tcccggatat
      301 cggctagccg gccatagagg gttccgtcaa tcgtttcaga caggaaccgg acagagccat
      361 cgccgagcag gaagttcgca ccgcccgggt gcttgctccc gaaagcgtaa tcggccaggg
      421 tcacagagcc aatgggcatc gtatctttgc agttaatgac tgcgtaagga ccagcgacac
      481 gtgcccaaga gctaattcgc cactggccag cccagtcatt gttgccacca gcccaaaggg
      541 gaaacatgcg gtccgtctgg gttcgcgaga tattggggct ttcagaaacc tcaccaacgg
      601 cgatgacgtt gctcgtaccg tcaatgatct ctgagaaggt tgtcacgaaa tggtagtcat
      661 ttgtctgggc caatcggaac atcccgtttt gaagagacgc cgatggctcg ctccagctaa
      721 gtcctccact catgtattga ttaacccatg gctcatcgct gccgaggcta cagcagtaat
      781 tggactttcc gtaaccgttg ttgtcttgct tcggcaaaac atcgctgggg caaatgtagc
      841 tgttgataac gctcttggca ccaacattgt tggcgtggtt agggttgcca tgatcacttt
      901 tcacctgggt ccaccagttg tacgagtcgg tattccgcac agggtggccg ctcggtaggg
      961 cgggcgagac accgggaatt tccctaatcg caccatggac tttaaggttg tcgccgccgg
     1021 ggatgtaaac gagggccgcg ccgccgccca caagggtatc atacatcgac ttctgttcca
     1081 taaacggaag gatgtacgcg ctccaggagt agttgtcatt gtcatcatcc ggctgaccta
     1141 ccggaaacac cccataaaca tcgtggaaat tgtgcaaggc caagccaatt tgcttgagat
     1201 tgtttgtgca ctgtgaccgt cgcgcagcct ctcgcgcagc ctggaccgcc ggtaaaagca
     1261 aggcaatcaa aattccgatg atggcaatca ccactaacaa ttcgacaagc gtaaacgctc
     1321 gtctcaccat gcgatttgaa cctttcatga aacacctcca aagagtgagc aaacgagacc
     1381 aaactgaaca tttgaacact aacgatccaa ccaacaccct gcgttgcctg ccctgaattg
     1441 cacaaaaaat caacgcctca gcctcggtgc caagggtggg gaactactcg cggcaccgcc
     1501 ccttgccgac gcgcacacaa aatgggatgg atcggaagaa tcccttttcg aaacctatct
     1561 tccgacctgc tggccagccg cacgtcttcc cggccgttaa cctccgagaa gagccgcatg
     1621 tcatgcgcct tctatttgga ttatagtata tgccgcctta ttggtgaaca atctgaagcg
     1681 aaaaaagttc cgccgggttc cttttttagc caccggacat actcggtact ggggactaaa
     1741 aaggcaagga tgacttggag gacgttcggc attgcaaggg aaaaacactc gctttattgg
     1801 gccccttcgg acaaatcggc gcacacaagt tcctcgtcat ttcggttaaa aacgtgcctg
     1861 tgggcaaatg ccggatgcga ccacgtgacg cccgggcccc gacttagttg cccacgggtg
     1921 ggggccagta gccgtgcctt agaaatgacg tggacacctt gtggcgttaa ttctgtgatc
     1981 attagctcgc ctcgatcgtt gaacatccag accacatccg cctgctgcac catgtgtaac
     2041 gttccccacc gaagttgcga ggtaagtgtc aagtcctccc agatccgatc gccactcctg
     2101 gcgtccaagc agcgaaattg tccatagctg tcaacgccaa aaatccaatt ttctttaata
     2161 accggatttc ccatgagtat gtgtaaagaa tcagtgttaa tttcgttttg gccctggcgt
     2221 cgccaaattt tctgcacatg caagtcatta gaatcaacat gtaacatgag tgacccgcaa
     2281 tcaaaacaac tgataaatag gcggttcgtg cttgagtccc agacaggtgt gatgatgcca
     2341 tcaatccatc gtcgatgagg gaaaggtacg tcccacagta gttttcccgt aagggcatcg
     2401 agtcccgcaa ggcgttcgcc ggtccagcac acaacgacgc gccgtccggc ctgggtgatc
     2461 gtgatcgggg cagaatacga ggcctgatct gggagggcct tccagcgaag ttcgcccgtc
     2521 tttcggtcga gcgcgacgac gcagccatct tcgagaccaa tttggacgat gagaaggtcg
     2581 ccttccacga gaggcgcagc cgcgacaccc cacgtgggga cacgaacttc ccaatcttga
     2641 ccagcccgct ttttccagac cactgtgccc gtttcggcgt ccagacagtg caggtcaccc
     2701 atggtcccca gggcgtaggc tagcccttca tgaacagtca cggaggccct ggggcccgtc
     2761 cggtacgtga cgtttcgata gacacaagga tagctgaagg tccacagaca ctgccccgtt
     2821 tgccagtcga aggcatgcac acgttcgacc tcttccggct cggtgagtcg atcggtgaca
     2881 aagactcggc catcggccac cgttggacca ctatacccgc ccgcgatctt ggtccgccat
     2941 cgcaatttaa cctccggtcc agaaaactgt cgggtgatgc ccgtttctcg ccagatgcca
     3001 tctcggtttg gcccccgcca ctgcggccaa tcatcggcgg aggtcattga agatatgaaa
     3061 acggcgatag cgcagcccac cgaggcgatc cagcgaacgc accaggccga gaggtgcccc
     3121 aacacagtgt gtgttctttt aaagtaagtt ttcacggtgg acggcctccc acagtcggat
     3181 ggaaagatcg aatgacacca cgcgtgctgc ttgcccatcg tgcaatcgtt gtg
//
LOCUS       DAOPTR010000402         3218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_23897_length_3218_cov_3.667404, whole genome shotgun sequence.
ACCESSION   DAOPTR010000402 DAOPTR010000000
VERSION     DAOPTR010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23897_length_3218_cov_3.667404"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..580
                     /locus_tag="PKI05_08490"
     CDS             74..580
                     /locus_tag="PKI05_08490"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOA52283.1"
                     /translation="MRTSVFVACVAMVACSSQLWAVESKQSPAPRVIDVALHDGGLLL
                     GQVVTPEGLPVPNAAVTVVSGDQVLGTATTDAQGRFAFRGLKGGVYRLNAANGTGMYR
                     LWAARTAPPGAQDSTLIVSGQDLARGQVLPRVRTWMRNPWIVGGAIATAIAVPVAVAE
                     HDEEPSSP"
     gene            complement(774..1751)
                     /locus_tag="PKI05_08495"
     CDS             complement(774..1751)
                     /locus_tag="PKI05_08495"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOA52284.1"
                     /translation="MSRMTRRDWLNTTALGVGLWSMGALAGRKPVSGEEAARPARRFQ
                     AALVCSAIGVRADQRQAIEYAARFGFEAVEPQPDFLSRLSQEELADLRQEMQEKGLTW
                     SAAGLPVDFRGDIDAFTRSLRRFPQFCETLQRAGVDRVGTWISPSHNERTYLENFRLH
                     AKRLRAVANIAGGYGLRLGLEYVGPKKSWSAGRYPFIHTMREMQELIAEIDHPCVGLT
                     LDSWHWYTAGETVEDLRRLPGHRVVIVHINDAPSGIPVEEQIDSVRELPCATGVIDLK
                     GFLGALVEIGFDGPVYIEPFKKELRQLPPEEALQLTAEAMQKAIALVGN"
     gene            complement(1902..>3218)
                     /gene="rimO"
                     /locus_tag="PKI05_08500"
     CDS             complement(1902..>3218)
                     /gene="rimO"
                     /locus_tag="PKI05_08500"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOA52285.1"
                     /translation="SNALPIVSTDPEVQASPARSSAKPLGTCSLIALGCPKNLVDGER
                     MLGLLRDAGWQLTTDPQEADVVVVNTCGFLRSAREESFAVIEEMLKLKRKGKIRGVIV
                     TGCLVEKDREALLDRFPEVDQLVNLFARDEITQAAARIQAGLVEQRTVFQPASVVPLD
                     DRRRLRVTPRHLAYLKISEGCDRLCTFCLIPHIRGRHVSKPLESIVEEARELVAEGVR
                     ELILVAQDLTYYGLDLAGRPLLAELLRRLRELPGLRWLRLMYLYPMHITDELVEVLRE
                     GEPVLPYLDLPLQHISDRILKRMKRRVNRRETEALLAKLRESIPNLVLRTTMIVGFPG
                     ETEAEFQELVEFVKTQRFERLGVFEYSREPGTPAARLQDHLPRRVIRARREHLMAVQQ
                     EIAFEYAQGQVGRQMTILLDQPYPERRHVFIGRTYADAQRSTAWCM"
BASE COUNT          657 a         1001 c          916 g          644 t
ORIGIN      
        1 tgtcgatttt gcagcgtttc ccgtaccccc gggcaagggt tgcttcatct cgtacggagg
       61 cggagtaaga aagatgagaa cttcagtttt tgttgcgtgt gtggcaatgg tggcctgctc
      121 ttcccagctt tgggcggtgg agagcaaaca atccccggcc ccgcgggtca ttgacgtagc
      181 cctccatgat ggtgggttac ttttaggaca agtggtgacg cccgagggtc tgccggtccc
      241 gaatgccgcc gtgaccgtgg tcagtggtga tcaggtcctt ggcacggcca cgacagatgc
      301 ccagggacgc tttgccttcc gcggcctgaa gggcggtgtc tatcgtctga atgcggccaa
      361 cgggaccggg atgtatcgcc tatgggcggc acgcacagct cccccaggcg cccaggattc
      421 cacgttgatc gtctcgggtc aagaccttgc ccgcggtcag gtcctgccgc gtgtccggac
      481 atggatgcgg aatccctgga ttgtgggtgg ggccatcgca acggccattg ccgtcccggt
      541 tgccgttgcc gagcacgacg aagaaccttc tagcccgtga ccggagaatc gccgagcggg
      601 ggtcaggcac gccttcaaat aatgggaagc aggaagtgcc tagtaccacg cagtaacctc
      661 cgtattccct tgccgacacc tcccgatcct tgcagatgat ggccctgttg gttgccccac
      721 agggccatcg tcttttcttg gccgcacggt cggttcgcaa gacctttgaa atgtcagttt
      781 ccgactaagg caatcgcttt ctgcatagcc tcggccgtaa gttgcaaggc ctcctctggg
      841 ggcaattgtc gtagttcttt tttgaacggc tcaatgtaaa ccgggccgtc gaaaccgatt
      901 tccaccagtg cccccaagaa ccctttcagg tcaatgacgc ccgtggcaca ggggagctcg
      961 cggaccgagt cgatctgttc ctccaccgga attccactcg gggcatcatt gatatgaacg
     1021 atgacgacac gatggccagg gagcctgcga aggtcctcca ccgtttcccc tgctgtgtac
     1081 caatgccagg aatcgagcgt caggccaacg cagggatggt caatttcggc aatcaattcc
     1141 tgcatttccc gcatggtgtg aatgaacggg tacctgccgg ccgaccacga tttcttcgga
     1201 ccaacatact ccaggccaag ccgcagccca taacccccgg caatattagc caccgcgcga
     1261 agtcgtttgg cgtgcaagcg gaagttttcc agatacgtcc gttcgttgtg gctgggcgaa
     1321 atccacgtcc ccacccgatc cacgccggcg cgttgcaggg tttcgcagaa ttgggggaaa
     1381 cgtcgtaagg agcgggtgaa cgcgtcaata tcaccgcgaa aatcgacagg aagcccggcc
     1441 gccgaccagg tcagcccttt ttcttgcatt tcctggcgga ggtcggccag ctcctcctgg
     1501 gaaagtcggc ttaggaaatc aggctgcggt tcgacggctt caaagccgaa gcgggctgca
     1561 tattcaatcg cctgacgctg atccgcccgc acaccaatgg ccgaacacac aagcgccgcc
     1621 tgaaaccgtc gcgcgggcct cgccgcttcc tccccactta ccggctttcg tccggccaga
     1681 gcacccatcg accacagtcc gacaccgagg gccgtcgtgt ttaaccagtc acgccgtgtc
     1741 atgcgactca tcgcaaacct cccaccaggt cataagaatc taaagccatc gaagacatca
     1801 tcccgcaggt tgttcatcaa gaggtgccgc caccaggtcg tagccctggg ctgccacgat
     1861 ttcgcacgtg acaaactgtc ccggctgaag gtgttttccc gtcacataca ccacgctgtc
     1921 gatctctggg catcggcgta tgtcctaccg atgaagacat gccttcgctc tggatacggc
     1981 tggtccagga ggatggtcat ctgacgtccg acctggccct gggcatactc aaaggcgatc
     2041 tcctgctgga cggccatcag gtgctcccgc cgtgcccgga tgacccgacg cggcagatga
     2101 tcctgaagcc gcgccgctgg tgtccccggt tcacgagaat actcaaaaac ccccagtctt
     2161 tcaaaccgct gggtcttgac gaactccact aactcttgaa attctgcctc cgtttcgccg
     2221 ggaaagccca caatcatcgt cgtccgtagg accaggttcg ggatcgactc ccgcagtttc
     2281 gctaacaggg cctcggtctc ccggcgattg acccgccgct tcatccgctt caagatgcgg
     2341 tcgctgatgt gttgcagggg cagatcgaga tacgggagaa ccggctcacc ctcgcggagg
     2401 acttccacaa gttcgtcggt gatgtgcatg ggatacagat acataaggcg aagccaccgc
     2461 aggcccggca attcccgcaa acgccgcaaa agctcggcca gcaagggacg acccgccaag
     2521 tcgaggccgt agtatgtcaa gtcctgcgct accaggatca attcccgcac gccctcggca
     2581 accaattccc gggcctcctc cacaatactc tcgagcggct tactcacatg gcgtccccgg
     2641 atgtggggaa tcaggcagaa tgtgcaaagg cggtcacacc cttcggaaat cttcaggtag
     2701 gccaaatgcc ggggtgttac ccgcaagcga cgacgatcat ccaggggaac gacggaagcc
     2761 ggttggaaga ccgttcgttg ttcgaccaga cctgcttgaa tccgcgctgc ggcctgcgtg
     2821 atttcatcgc gggcaaagag attgaccagt tgatccacct ccgggaagcg atccagaagg
     2881 gcctcccggt ctttctccac cagacagcca gtcacaatga cgccacgaat cttgcccttc
     2941 cgcttcagct tgagcatctc ctcaatgacg gcgaacgatt cctcacgggc cgagcggagg
     3001 aaaccgcagg tgttaacaac gaccacgtcg gcctcttggg ggtctgtggt cagctgccaa
     3061 ccggcgtcgc gcaaaaggcc caacatccgc tcgccatcga caaggttctt gggacagccg
     3121 agggcaatga ggctgcatgt ccccaggggt tttgccgagc ttctcgcagg ggaggcctgc
     3181 acttcgggat cggtcgaaac aatgggtaaa gcgttgga
//
LOCUS       DAOPTR010000403         3216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_23912_length_3216_cov_4.286935, whole genome shotgun sequence.
ACCESSION   DAOPTR010000403 DAOPTR010000000
VERSION     DAOPTR010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23912_length_3216_cov_4.286935"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PKI05_08505"
     CDS             <1..499
                     /locus_tag="PKI05_08505"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOA52286.1"
                     /translation="PPNAPPHGGMFPQRNRIISGMSVGVVIVEAGENSGALITARHAV
                     EQDREVFAVPGRADSPNARGCNRLLRDGAKWVESPQDVLDGLGPLVRPVERPDGTVVR
                     RPLELKLSPLEQQVLQTIRDDPTAIDEVIAVTGLAASQVLATLSVLEMRGVIRRVGGT
                     NVVRR"
     gene            complement(709..2325)
                     /locus_tag="PKI05_08510"
     CDS             complement(709..2325)
                     /locus_tag="PKI05_08510"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOA52287.1"
                     /translation="MTVFSRRQQVLWFLALISLLVPYLGLMIIGGYALWQNGWFWIYG
                     SVTAGVTALVCGLAFYLHKRRDLQALEPLKFECPQDWPPREQAAWSKVEHLAQQATRG
                     EIPVESIDDFRALLVRLVREIAQHYHPEHDDAYLDTPLPQLLKIVEKVCQELRQVVQN
                     HVPGAHILTLRDWRTIKEAYDFFEPLYRLIYWAYRIGSIPVSPVSALWREIRGFVAGQ
                     ALNLSVKELKAELVSYVIHRMGFYLIELYSGRIILDDKEFAEVRRQAEQRELQTAEPE
                     VPDTPPEFIRIVIVGQLKAGKSSLINALRGTWQAQTDVLPCTDQVQCYLMRPLAEGDV
                     NDPRPVLPGLLVLVDTPGYDSLGDGRNFAPSLDEVKKADMVLLVCSARTAGRKADHEF
                     LQAVRQWFQAHPHWIMPPLIVVGSFIDELRPRHEWNPPYRWRDVKEGESLSPKEQALQ
                     LFLEALRRDLELASGDPVIPVCTHPERTWNIAEGLIPTVAAYWEKARAVRDNRHREAY
                     QKTPYRLLWRQVKNATGSTIRLALKKIGIR"
     gene            complement(2337..>3216)
                     /locus_tag="PKI05_08515"
     CDS             complement(2337..>3216)
                     /locus_tag="PKI05_08515"
                     /inference="COORDINATES: protein motif:HMM:NF016983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HOA52288.1"
                     /translation="MVVMRVMDHAQGSVRAVVEDLRRRKPDWPIIVVQTCLHEGYTWR
                     EPRHILPYPFDKEPLPSEVPPDLTRSLLAQRAWFRGETIRYVAIDFTLPEDGFEPQFY
                     GLQQLWRAIEEALPLGLWSFLKEGGPLSQSLAEPYAQAAEQTILAHTVLAAGAAAVPV
                     AAIDIPLIVAIQFHMAQRIAKIYGQSLTGDRWLDLMGTLGWSFLTRLGVRFISRELLK
                     LLPWVGVPVAATFMGASTYALGRMLAWYFAEIKHGHLPDQHALRRMYEKEWERARDRL
                     GGVFSSSSKEGPEKDR"
BASE COUNT          669 a          998 c          939 g          610 t
ORIGIN      
        1 cccacctaat gccccgcccc acggcggtat gttcccgcaa cgcaatcgca tcatcagcgg
       61 catgtccgtg ggcgttgtca tcgtagaggc cggggaaaac agcggggccc tgatcacggc
      121 acgccatgcc gttgagcaag accgcgaggt ctttgccgtt cctggtcggg ccgactcgcc
      181 caatgcccgc ggatgcaacc gccttctccg cgacggggcg aaatgggtcg aatcaccgca
      241 ggacgtcctc gacggcctag gcccgctcgt ccggcccgtg gaacgtcccg acggcaccgt
      301 ggttcgacga cccctcgaat tgaaactcag tccgttggaa cagcaggtcc tgcaaacaat
      361 ccgcgacgac ccaacggcca tcgacgaggt gattgccgtc acagggctag cggcctcgca
      421 agtgctggca accctcagcg tgttggagat gcgtggtgtg atccggcgcg ttggggggac
      481 caacgtcgtc cgccgctgaa tcgacaggat gggcacggca acaaagggcg gccgtgggcg
      541 ctctgcgctt ctggggcagg cacgggggcc tgcccctaca cgtttcaccg ggagggtcat
      601 cgtcctcgat gaccaaatcg ctgcaccgcg caaacgtccg ccgcgtccac acgcacgagg
      661 acgccgaccg tccctccccc ggctctcaaa gggaaaaaca ccccatcgtc agcggatccc
      721 gatctttttc agggccaatc gtatggtgct gcccgttgca tttttcacct gccgccaaag
      781 caagcgatat ggtgtctttt gataggcctc tcgatggcgg ttgtcgcgga cggcccgcgc
      841 cttctcccag tacgcggcga cggttgggat caagccctcc gcgatgttcc aggtcctctc
      901 gggatgcgtg cacacaggga tcaccggatc gcccgacgct agctccagat cgcggcgaag
      961 ggcttccagg aaaagctgaa gggcttgttc ctttggcgaa aggctttccc cctcttttac
     1021 atctctccag cggtaggggg gattccactc gtgccgaggc cgaagctcgt cgataaacga
     1081 cccgaccaca atcaggggcg gcatgatcca atggggatgg gcttgaaacc attgccgaac
     1141 cgcctgcaag aactcgtgat ctgctttgcg gcctgccgtc cgcgccgagc acaccaagag
     1201 gaccatgtcg gcctttttga cttcatccaa ggacggcgca aaattccgac catcgcccag
     1261 gctatcgtaa cccggtgtgt ccaccagtac gagcagaccc ggcaggaccg gtcgaggatc
     1321 gttgacatcc ccttctgcga gcggtctcat caaatagcac tggacctgat cggtacaagg
     1381 aaggacatcg gtttgggcct gccatgttcc gcggagggca ttgattaggc tggatttgcc
     1441 tgccttcaat tggcccacaa tcacgatccg aataaattcc ggcggggtat cgggcacttc
     1501 tggttcggcg gtctgcagct ctctttgctc ggcctgcctt cgcacctcgg cgaattcttt
     1561 gtcgtcgaga ataattcgcc cggagtatag ctcaatcaag taaaagccca tcctgtggat
     1621 cacgtaagag accaattccg cttttagctc tttgaccgag aggttaaggg cctgtcccgc
     1681 cacaaagccg cggatttctc gccacaacgc gctaactggc gaaacgggga tgctgccgat
     1741 gcggtacgcc caatagatca gccgataaag cggctcgaag aaatcgtacg cctccttgat
     1801 cgtccgccag tcgcggagag tgaggatatg cgcgccgggc acgtgattct gcacgacctg
     1861 gcgcagttcc tgacaaacct tctcgacgat cttgaggagc tgcggcaggg gagtgtcgag
     1921 ataggcgtcg tcgtgctccg gatggtaatg ctgggcaatc tcgcggacaa gtctgaccag
     1981 caatgcccga aagtcatcga tcgactccac ggggatttca ccccgggtag cttgttgggc
     2041 aagatgttca accttgctcc aagccgcttg ctcccgcggg ggccaatctt ggggacattc
     2101 gaatttgagc ggttctagtg cctggaggtc ccgcctttta tgcaggtaga atgctagacc
     2161 acagacaagt gcggtaacgc ctgcggtaac gcttccgtag atccagaacc acccgttttg
     2221 ccagagggcg tatccgccga taatcatcag cccaaggtag gggacgagca aactgatgag
     2281 agcgaggaac cagaggactt gttgccggcg tgagaacacg gtcattcctc ccttgatcaa
     2341 cggtcctttt ctgggccttc cttggaggac gaagaaaaaa caccgcccag gcggtcgcgc
     2401 gctcgctccc attccttttc atacattctg cgtagggcat gctggtccgg caaatggccg
     2461 tgtttgattt cggcaaaata ccaggccagc atccgcccca gggcataggt cgatgcaccc
     2521 ataaacgtgg ccgccacggg cacacccacc cagggtagga gtttcaacag ctcccgcgaa
     2581 atgaatcgca ctcccagccg cgtgaggaat gaccagccca atgtccccat cagatcaagc
     2641 cagcgatcac cagtgagcga ctgcccgtaa atcttggcga tccgctgggc catgtggaac
     2701 tgaattgcca caatcaaggg gatatcaata gcggcaacag ggacggccgc ggccccggct
     2761 gccaataccg tatgggccaa gatggtttgc tcggccgcct gggcataggg ctctgctaac
     2821 gactgcgaca ggggaccacc ctccttcagg aagctccata agccgagggg cagtgcctcc
     2881 tcaatcgccc gccacagttg ttgcagtccg taaaactgcg gctcaaaacc gtcctcgggc
     2941 aaagtgaagt cgatggccac gtagcggatc gtctcacccc gaaaccacgc ccgctgggcc
     3001 aggagagagc gcgtcaaatc ggggggcacc tcggagggca gcggttcctt gtcaaacgga
     3061 tagggcaaaa tatgccgggg ctcccgccag gtgtaacctt cgtgcaagca cgtctggacg
     3121 acgatgatcg gccagtcggg ctttcggcgg cgaaggtctt ccaccacggc acgcaccgaa
     3181 ccctgggcat gatccatgac ccgcatgacc accatc
//
LOCUS       DAOPTR010000404         3212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_23955_length_3212_cov_3.905923, whole genome shotgun sequence.
ACCESSION   DAOPTR010000404 DAOPTR010000000
VERSION     DAOPTR010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23955_length_3212_cov_3.905923"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PKI05_08520"
     CDS             <1..352
                     /locus_tag="PKI05_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52289.1"
                     /translation="LASRETKLTLAGLRDTVKEILGENFGRVIDGNELTSPTGHQMFH
                     VHVAGQVSDVPIHWIYYVMTTPDGKNHAVVFTVEEKTLERFGNGDRELVDGFEFLQPA
                     EVPRISQGPPTPIR"
     gene            557..629
                     /locus_tag="PKI05_08525"
     tRNA            557..629
                     /locus_tag="PKI05_08525"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:590..592,aa:Cys,seq:gca)
     gene            1030..>3212
                     /locus_tag="PKI05_08530"
     CDS             1030..>3212
                     /locus_tag="PKI05_08530"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOA52290.1"
                     /translation="MSRARWVLLTCTMLLGFLTGSALIVFQKDAAERRSFLDQVKSGS
                     SIDIADIRGLRGSPEDLKTQAYQHTRRKLAKLKERWPGCRFVYLLGRRPDGQVFFFAD
                     SEDPEGATASLPGEPYHEASDRIRCSFDHGEPFTEGPLADRWGLWVSAIVPIVDPETN
                     SVAAVLGADIDGWQWYKDVVAGALPYVVALFIAVCVLESSILLLVSWRIRKCKTRSRT
                     SKNVAVSCNTMRGLIVAIGVLGVIFLGVVVWETCSWTKYLVEMSAAEKTRTLARFNRE
                     LRDYFASYVRPEFLKRLGPDEFLPEVLSTSYVARHVFEGVNEERDSYVRFTARNPRNP
                     LNRPTPGEAWLLEYFEKNPSADSWSGRLKFFEGGQPYYVVAYARRFEESCLRCHGRPE
                     DAPSGLIKRYGDQAGFHNRVGDVSLELMAVPMDRAMSRASQQVIYYLLGAMGVCGLFI
                     GGVIGLVVLHRVQERRLQQELHKGREFLAATLRSIGDAVISCDCHGRIVSLNSVAEKL
                     TGWSNEEALGVSVEDLFSIFDSRTGERLPNLVHQVISCGQALELAGHIKLMTRDGRSY
                     DIAESCAPIRDLLGQVIGAVLVFRDVNEQYRQQDKLRESQDQLRAITDSVQDAIIMID
                     PDGKITFWNPAAEKIFGYTAREVMGLSVHEFLCLDEDRQRFLRSYPQFVENGEGPAVG
                     RTLELRARRKDGQIITVELSLGAVKLQSGWHAVAVVRDITQRKRN"
BASE COUNT          695 a          742 c          957 g          818 t
ORIGIN      
        1 gttggcttct cgggaaacga aacttacact tgctggcctc cgtgacacgg tgaaggaaat
       61 tcttggtgag aacttcgggc gagttatcga cggtaacgag ctcacaagtc cgacaggcca
      121 ccagatgttt catgtgcacg tagctggcca ggtttcggat gttcccatcc attggatcta
      181 ctacgtgatg acgacgcctg acggtaaaaa tcatgccgtc gtcttcacgg tggaagagaa
      241 gacgctggag cggtttggaa atggtgaccg ggaattagta gatggttttg agttcttgca
      301 accggccgag gttccgagaa tttcccaagg cccgccgacg ccaattcgat gaagttatct
      361 ctagaaggtg ggagttgtcc cccaagttcg ccttcctgcc aaacgtgaca gtagatgact
      421 tagcaaagga gcacgttgag ttggtcaatg tcgatttccc gcgcgtgctc gagcgttttg
      481 cgatgcctct tgccttgcta gggatgacgg gacataataa ggttgaagtt gcataaaagt
      541 gaagtgtcga aatgttggcg ccgtagccaa gcggctaagg cagcggattg caaatccgcc
      601 attcgtcggt tcgaatccga ccggcgcctc ttagcaggaa gagtcaatct ctctcgatct
      661 tcctcgtgaa tgtggcttct ctgatttggc cacggcttta agggcgaaag actagtcttt
      721 tcgcaaagaa gaccgcggtc cagtctcagg acccgacgat tcggtttact cggaaaacgt
      781 gttactggtc gggtaatcct cacttttgga atccgcattc tccaggggca cgcgcggtta
      841 ttgcgctcca acgtgtctgg aaatgcaact gttcttgtgg aaaccagttg gtagtatccc
      901 accgggtacc gggcggcgcc ctatagtttc ttgcatggaa cctttgacag ctcgcagtct
      961 gtgttgccaa tcctgcgaga gtggtgatcg gtgagagtgc ttggggtgat tactggaaga
     1021 tcgggtgcca tgtcacgggc tcgatgggtg ttactgactt gcacgatgct attaggcttc
     1081 ctcaccggaa gtgccttgat cgtctttcag aaggacgctg cggaaagacg ttcttttctc
     1141 gatcaggtca aaagcggcag ttccatcgat attgccgaca ttcggggcct tcggggatca
     1201 cctgaggatc tcaagacaca ggcctaccag catacgagaa gaaaacttgc caagctgaaa
     1261 gaacgatggc cgggttgtcg gtttgtttat ctcttggggc gaagacccga cggccaagtg
     1321 ttcttcttcg ccgacagcga ggatcccgag ggggctaccg catcactacc cggcgaacct
     1381 taccacgagg cttccgaccg aatacgttgc tcctttgatc acggagagcc gtttactgag
     1441 gggccactag cagaccgttg gggcttgtgg gtctctgcga ttgtgcccat tgtggatccg
     1501 gagactaatt ccgtcgcggc cgtccttggg gccgatattg acggctggca gtggtacaag
     1561 gatgtcgtgg ctggggcttt accctacgtg gttgcgctgt tcattgctgt ttgtgtcctg
     1621 gagtcgagta ttttgctact tgtttcctgg agaattcgaa aatgcaaaac acggtcacgc
     1681 acgtcgaaga atgtggcggt ttcctgtaat accatgagag gcctcattgt ggcgattggt
     1741 gttcttgggg taatattcct cggcgtggtt gtgtgggaaa cctgcagctg gacgaaatat
     1801 cttgtggaaa tgtccgcggc cgaaaaaacc agaacgttag cgcggtttaa cagagaattg
     1861 cgcgactact ttgcctctta cgttcgtccc gaattcctga aacggcttgg accagatgag
     1921 tttcttcccg aagttttatc tacctcctat gttgcgcgtc atgtttttga aggggtgaac
     1981 gaagagcgag atagctatgt tagattcact gcaaggaatc cccgcaatcc gttgaatcgg
     2041 ccgacccccg gtgaggcgtg gctgctggag tattttgaga aaaatccaag tgctgattca
     2101 tggtcgggaa gacttaagtt ttttgagggc ggacaaccgt attatgtcgt cgcctatgcg
     2161 cgacggtttg aggaaagctg cctccggtgc catgggcggc cggaggatgc tccttcgggg
     2221 ctcatcaagc gatacggtga ccaggctggc tttcataaca gagtgggtga tgtgtctttg
     2281 gagttgatgg ctgtgcccat ggatcgggcc atgtctcgcg cttcgcagca agtgatttac
     2341 tatttgcttg gggccatggg agtgtgtggg ctatttattg gaggtgtcat tggtctagtc
     2401 gttttgcata gggtccagga gagacggctt caacaggaac tccataaagg tcgtgagttt
     2461 ttggctgcta cgctacgatc cattggagac gctgtcatct catgcgactg ccacgggcga
     2521 atcgtgtcgc tcaattctgt tgcggaaaaa cttacaggtt ggtcgaacga agaggccctg
     2581 ggagtatccg tcgaagatct cttctccatt ttcgacagtc gtacgggcga gcggcttccg
     2641 aatcttgttc atcaggtgat tagctgtggc caagcattgg aattggctgg tcatatcaag
     2701 ctgatgacac gagatgggcg ttcgtatgat attgcggaaa gttgtgctcc gatccgtgac
     2761 cttctgggcc aggttatcgg ggccgttcta gtgttccgag atgtcaatga gcagtaccgg
     2821 cagcaagata agctccgcga gagccaggac caattgcggg cgatcacgga ttctgttcaa
     2881 gatgcgatca ttatgattga cccggatggt aagatcacct tctggaatcc ggcggcagaa
     2941 aagattttcg ggtacacggc gagagaggtc atgggactca gcgtccatga atttctctgc
     3001 ttggatgagg atcgccaaag attcttgcgt agttatccac agtttgtaga aaacggggag
     3061 ggcccggccg tcggtcggac attggaactg cgagcgcgtc gcaaggatgg acagattatc
     3121 acggtggagc tgtcactagg ggcagtgaaa cttcaaagtg gttggcatgc cgtggcagtt
     3181 gttcgtgata ttacccagcg aaagcggaac ga
//
LOCUS       DAOPTR010000405         3212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_23956_length_3212_cov_3.731391, whole genome shotgun sequence.
ACCESSION   DAOPTR010000405 DAOPTR010000000
VERSION     DAOPTR010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23956_length_3212_cov_3.731391"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2085)
                     /gene="pilM"
                     /locus_tag="PKI05_08535"
     CDS             complement(<1..2085)
                     /gene="pilM"
                     /locus_tag="PKI05_08535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOA52291.1"
                     /translation="MPKSEACWGIDIGNASLKALRLRPGESPSFVVADAFDYIEYPML
                     LTQPAADPPSLIETALRQFLSRNEVRGHKVAISVSGATGLARFIKLPPVELSKLPDVV
                     RYEAKQQIPYDLNSVVWRYQRLDAGGSESGFTLGSTVGIFALKRDQVEAAIQPFANVG
                     IEVDVIQLSPLAIFNYARFDAMPDLPPPDEYDPDNPPPSIVVISMGTDSTDLVITNGY
                     RVWQRNIPIGGSHFTKALSKELKLTFSKAEHIKKNPTTHSDPKAVFQAMRSVFTELLS
                     EIQRSISYYNTIDRAVEISRGIALGNPMKLQGLRAFLEKGLGFPLEHVDQFERLQGPD
                     VLQSPVFRENLSCFGVTYGLALQGIGRAYMEINMLPDEIVQERFIRAKKPWALAAATT
                     LLIGCTVSFAAQSLALSRVSPERFAAAERTAEQVLKTVQQYQSELASVESEFNRIKSI
                     GQNLTSSIEGRLLWLELLSAINQCLPQYRPQAGAPADVVDISAVTDTEKQQQILEQRD
                     EIHITSLEAIRTEQLETWWQRMGGWYRKPGETDTEPAETAQATAATGTATPGSPGAMM
                     GSMTSTYGMSGMYGPESETGGYGPTGGGLFASVPARTPPPSDVQGPSGSGYVIRLTGY
                     HFHNSDVAGPNQGAQYVRNTLIRNLEEGKFWINGKWVTAKELGIGYPILVNPGRIEVI
                     RVADPNYRPPAGT"
     gene            complement(2460..>3212)
                     /locus_tag="PKI05_08540"
     CDS             complement(2460..>3212)
                     /locus_tag="PKI05_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52292.1"
                     /translation="GSFFLFCEDPRSALVVRAAQNREADQLRGTAVRLHRCIIRGDGD
                     LFRSTYCQPVRVTITESFVSVAGRLLDMLGTERSPASDAGVHLELQRLTAWTAKGLIR
                     QQQLTYQPYLQPIRVESEHCIFVASPNSALIEQHVPNVDGALRLLTWFGQRNFYEQFS
                     QFWLVVTGAQHSVPLQLSFDQWKAYWQTEHEQEPAWGAIPWQAPLPLDVPPHLHRPSD
                     FAIMDPSWDDIASDIANRAGCPVDQLPFVPSR"
BASE COUNT          715 a          950 c          807 g          740 t
ORIGIN      
        1 cgtcccggcc ggcggccgat aatttggatc ggccacacgg ataacctcga tccgcccagg
       61 gttgaccaag atgggatagc caatccccaa ttccttggct gttacccact ttccgttgat
      121 ccagaatttt ccttcttcga gattccgaat cagggtattc cttacatatt gcgcaccctg
      181 attgggtcca gcgacatcgc tattgtggaa gtgataaccc gtaagtcgta tcacatatcc
      241 gcttcccgaa ggcccttgca catcggaggg aggtggtgtg cgggctggta cgctcgcaaa
      301 tagtccccct cccgtaggcc cgtagccgcc tgtttcgctt tccggtccgt acattccgga
      361 cattccatat gtggaagtca tcgaccccat catcgctccc ggactgcccg gtgttgctgt
      421 tcccgtggcc gccgtcgctt gagctgtctc cgcaggctcg gtgtctgttt ctcccggctt
      481 gcgataccat ccgcccattc gctgccacca cgtttcaagt tgttctgttc ggatggcttc
      541 aaggctagtg atgtggatct cgtcccgttg ttccaagatc tgctgttgct tttcggtatc
      601 ggtaaccgcc gaaatgtcca cgacgtccgc gggagcccca gcttgaggac gatactgtgg
      661 taagcattgg ttaatggcag aaagcaattc aagccagagg agacgacctt caatactact
      721 cgtcaggttt tgaccaatgc tcttgatcct attgaactcc gactctaccg aggcgagctc
      781 cgactggtat tgttgtaccg tcttcaaaac ctgctctgcc gttcgctcgg ctgccgcaaa
      841 tcgttccggt gaaacccggc tcaaagcaag agactgcgca gcgaaactga ccgtgcatcc
      901 aatcaggaga gttgtagccg ctgccaatgc ccacggcttc ttagcccgaa tgaatcgttc
      961 ttgtacgatt tcatccggta acatgttgat ttccatgtag gcccgaccga ttccctgtag
     1021 ggccagccca taagtaacgc cgaaacacga caggttttct cggaacacag gagattgcaa
     1081 gacatcgggc ccctgcaagc gctcaaactg atccacgtgc tccaggggaa atcccagccc
     1141 tttttcgaga aaagcccgca acccctgaag cttcatgggg ttacccaagg caattccccg
     1201 cgaaatctca acagcccgat ctatcgtatt gtagtaactg atcgaccgct ggatttcgga
     1261 gagcaactcc gtaaataccg agcgcattgc ctgaaacact gccttggggt ctgaatgggt
     1321 cgtgggattc ttcttaatgt gctccgcctt ggagaacgtc agctttagct ccttactcaa
     1381 ggcctttgta aaatggcttc caccaatcgg gatattccgc tgccacaccc gatagccatt
     1441 ggtgattacc aagtcggtgc tgtccgttcc catggaaatc acaacaatgg acggtggggg
     1501 gttatccggg tcgtattcgt ccgggggcgg taaatctggc atcgcgtcga agcgagcata
     1561 gttgaaaatc gccaatgggc tcaactggat cacatcgact tcgatgccaa cgttggcgaa
     1621 aggctgaata gccgcttcca cctggtcccg cttgagtgcg aaaatcccca ccgtggatcc
     1681 caaggtgaag ccactctcgc tgccccccgc atccagtcgc tggtagcgcc acacgacgct
     1741 atttaagtca tacggaattt gctgcttggc ttcatatctg acgacatccg gcagcttgct
     1801 aagctccaca gggggtagct taataaaacg tgcaagcccc gtcgctcccg agacagagat
     1861 tgcgacctta tggccacgga cctcatttcg tgacaaaaac tggcgaaggg ctgtttcgat
     1921 caaactcgga ggatccgccg ccggttgcgt gagaagcatc ggatactcga tgtagtcaaa
     1981 ggcgtcggca acgacaaagc ttgggctttc gccaggtcgc aaccgtagcg ctttaagcga
     2041 tgcattcccg atatctattc cccagcaggc ttctgacttt ggcatagacg acacctcatt
     2101 tatgggaccg ctcggccacc aagccctttt cctattgctc agaagaccct gttacccatg
     2161 tcccctgatt agacggtccg tctctccctg ctaccgaaaa gctccccgat ctgtccaaaa
     2221 aaattactat cattccaccc cttaatgtat ccccgcgcac ccccaggtgt caacttgcaa
     2281 tccgcttccg aagcgatttc ccgcaacgtt tgcccctacc ggctttccgg ccgccattcg
     2341 caccatcgaa tgtgacgtca cagctgttca ctgaaggaag aatcgccgac aagtttattg
     2401 tgcacccaca attaatgtct tttaatctcc ctaccggtct tccgttgaag aaaacggcgc
     2461 tatcgtgacg ggacgaaagg cagttggtcc acgggacaac cggcacgatt cgcaatgtcg
     2521 ctcgcgatgt catcccacga gggatccata atggcaaaat cgctgggtcg atggaggtga
     2581 ggagggacgt ccagtgggag aggggcctgc cacggtatgg caccccacgc gggctcctgc
     2641 tcgtgctcag tctgccaata agccttccac tgatcaaacg acagttggag ggggacagaa
     2701 tgctgagctc ctgtcaccac cagccagaat tggctaaatt gctcatagaa attacgctgc
     2761 ccgaaccatg tcaataagcg gagggctcca tccacattcg gcacgtgctg ctcaatcaag
     2821 gcactattgg gactggcgac gaagatgcag tgttcacttt ccacccggat cggctgaaga
     2881 tagggctgat aagttaactg ctgctgccgg attagtcctt ttgccgtcca agcggtcaga
     2941 cgttgcaatt ctaggtgcac gcccgcatcg gacgcaggcg accgttccgt tcccagcatg
     3001 tcgagcaacc ggcctgccac ggagacaaag ctttccgtaa tcgtcacccg taccggctgg
     3061 caatatgtgc tgcggaaaag atcgccatct ccgcggataa tgcaccgatg caaccgaacc
     3121 gcagtcccgc gcagctgatc tgcttctctg ttttgtgcag cccgaacaac gagcgcactc
     3181 cgtgggtctt cacaaaagag aaaaaaacta cc
//
LOCUS       DAOPTR010000406         3209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_23980_length_3209_cov_3.309448, whole genome shotgun sequence.
ACCESSION   DAOPTR010000406 DAOPTR010000000
VERSION     DAOPTR010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23980_length_3209_cov_3.309448"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="PKI05_08545"
     CDS             complement(<1..785)
                     /locus_tag="PKI05_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52293.1"
                     /translation="MLPNAIPLDTRVLILCAGEGERWGEYLGKPKQLAPIFGVPLLVR
                     TAGQVQHDFGVSPVIVTHDPRLHIPGCDSFCPEQRRWVVETFLSTACLWSSRTVVLLG
                     NVLYTDAAITICRAASGGLVIFGRQGASIFTGKRYGELFGLSFTRDYSDFVTHCLIQV
                     IGDATRGGRGKLWEFYFLAQREPPSGRPPIEFCEIHDYTEDFDSPEEYHYLKRLCEWA
                     LSSAPALRLLAILWSSVFCAPYASWHRWKQWWHDRRNTRRTTLP"
     gene            complement(1016..2137)
                     /gene="mnmA"
                     /locus_tag="PKI05_08550"
     CDS             complement(1016..2137)
                     /gene="mnmA"
                     /locus_tag="PKI05_08550"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOA52294.1"
                     /translation="MARVLVAMSGGVDSSVSAHLLKEQGHEVIGVFMRHGIRSPLHCV
                     NEGRNPTLPVLPGRKQGCCSAEDAEDARRVADRLGIPFYVVNFEEEFRQIVHYFVDEY
                     TRGRTPNPCIVCNNRLKFGKLFDYADALQADFIATGHYAQIIPTEDKNYGLFCGRDQS
                     KDQSYVLFGLRREDLPRLLFPIGNYTKAEIRKIATELGLRVANKPDSQEICFVPDKDH
                     ARFVKQYRPNVDTSGEIVTTRGEVVGHHGGLERFTIGQRKGLGIAFGEPRYVVRIEAD
                     TRRVVVGTREELARWDLTASGANWLIDPPAEPFPCQVKIRYRTPAVPATVTILSSDRF
                     LVRFHKPCYGVAPGQAAVCYQGTRVLGGGWIDSSPASNG"
     gene            complement(2366..>3209)
                     /locus_tag="PKI05_08555"
     CDS             complement(2366..>3209)
                     /locus_tag="PKI05_08555"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOA52295.1"
                     /translation="SGWGNRLSAAFWVKDNWCPSSRALGEKRNKLEKNRTISGMVTAW
                     RKTQLAIKRGIDIIGAAVALIILSPVMAATALAVWITMGRPIIFRQVRPGYRGRLFVL
                     YKFRTMRNAWSADGQPLPDAERLTPLGRWLRRFSLDELPQLWNVLKGDMSFVGPRPLL
                     VEYLPRYTPEQARRHEMRPGITGLAQVKGRAALKFSQRLAYDVYYVDHFNLLLDLKII
                     LLTLWKVPMGLLFDGAGQDVKVVDDIGLHPGQETLTWKLSATGETQLKLSDQTADTAK
                     RSAA"
BASE COUNT          730 a          903 c          867 g          709 t
ORIGIN      
        1 ggcagcgtgg ttctacgcgt gttccgtcgg tcatgccacc actgtttcca acgatgccaa
       61 ctcgcatatg gcgcgcagaa taccgacgac catagaattg ccaaaaggcg aagggccggt
      121 gcggatgaga gtgcccattc gcataatcgc ttcaaatagt ggtattcttc aggggaatca
      181 aaatcttccg tatagtcgtg gatctcacag aattcgatcg gcggacggcc cgatggaggc
      241 tccctttgag cgagaaagta gaattcccat agctttccgc ggccccctct tgtggcatcg
      301 ccgataactt ggatcaagca atgggtcacg aaatcggaat aatcccgcgt aaaggacaaa
      361 ccaaataatt cgccgtaacg tttgcctgta aagatactgg ctccctggcg cccgaagatc
      421 accaacccac cgcttgccgc ccgacatatg gtgatcgcag catccgtgta taaaacgttg
      481 cccaggagga cgaccgttct acttgaccat aggcacgccg tgctaaggaa cgtctcaacg
      541 acccagcggc gctgttccgg acaaaatgag tcacaacctg gaatgtgcaa gcgcggatca
      601 tgcgtcacaa tgacgggcga gactccgaag tcgtgttgaa cctgacctgc ggtgcggacg
      661 agcaagggaa cgccaaaaat cggggcgagt tgcttaggtt tgcccagata ctcgccccac
      721 cgttctcctt caccggcaca gaggattaag acgcgcgtat ctagtggaat ggcgttcggc
      781 aacacgatcg ttccatcctt gccttcattt ccattcggta tatcaagggc aagcctctca
      841 taaattttcg gatgtaatgt ggctgagtag tgcgaaattg cttgttcaag ggtactttga
      901 ttattgtcgg cgatagataa gattaatcga gattcgtgtc aagggcgaaa tgaatgtttt
      961 tccgctcccc ttttggttcc catcatgtgg ggtgttagct gtctgccagc gttattcacc
     1021 catttgatgc gggggacgaa tcgatccatc cgccaccaag aacacgagtg ccctggtagc
     1081 agaccgccgc ctgaccgggg gccactccgt agcacggttt gtgaaacctg acgagaaacc
     1141 gatccgacga gagtatggtg accgtagccg ggacggccgg ggttcgataa cggattttca
     1201 cctggcaggg gaatggctcg gcgggaggat caatcagcca gtttgccccg gaggctgtca
     1261 agtcccatct ggccaattcc tcccgtgttc ccaccaccac ccggcgggta tcggcttcga
     1321 tgcggacgac ataccgtggc tctccgaagg cgatacccag cccttttctt tgaccgatgg
     1381 taaaccgttc caatccgccg tggtggccaa cgacctcgcc tcttgtcgtg acgatttcac
     1441 ccgacgtgtc cacgttcggc cgatactgtt tgacaaatcg ggcatggtcc ttgtcgggaa
     1501 cgaagcaaat ttcttggctg tccggtttgt tggccacacg aaggccgagt tcggtagcga
     1561 tcttccgaat ctcggccttg gtgtagtttc cgatggggaa aagcagtcgg ggcaggtctt
     1621 ctcgccgcag cccaaatagg acatacgact gatccttgct ttgatcccgt ccacagaaca
     1681 atccgtagtt cttgtcctcg gtggggataa tctgagcgta atggcccgtt gcgatgaaat
     1741 cggcctgcaa ggcgtcggca tagtcgaaga gcttgccgaa ttttaagcga ttattacaaa
     1801 caatgcaggg attaggcgtt cggcctcgcg tatattcatc gacaaagtaa tgcacaattt
     1861 gccgaaactc ttcctcaaag ttcaccacgt agaaaggaat cccgagccga tcagcgacac
     1921 ggcgggcgtc ttcggcatct tctgcgctac aacaaccttg tttccgcccg gggagcacgg
     1981 gaagtgtggg attccgcccc tcgtttacgc aatgaagcgg gctacgaatg ccatggcgca
     2041 taaagacgcc gatgacctcg tgcccctgct ctttgagaag atgggccgag acgctgctat
     2101 cgacaccgcc cgacatcgcg accaaaaccc gggccatcaa gcattttttc ctggagagag
     2161 ccttccaaaa atgcgattcc aacctcaccc caatcctgtg agaaagacac tccatcccct
     2221 actgagattg tacgcgacga aggtaaaaga cagccaccga cacgaagcaa cagaccgccc
     2281 ttacaatttc ggcaatgcgg cgtcaagggt aggacgcaga cacactacca tgccgagacg
     2341 tgttagcggt gcccccagct caccctcagg cggcggatcg ttttgctgtg tcagccgttt
     2401 gatcagataa tttcagttgc gtctcgcccg ttgccgaaag cttccaggtc agtgtttcct
     2461 gacccggatg gagaccgatg tcatcgacaa ctttaacgtc ctgcccggcc ccatcaaaca
     2521 aaagacccat ggggaccttc cacagcgtga gtagaatgat ttttaggtcc agtagcaggt
     2581 taaagtggtc cacgtaatag acgtcgtagg ccaatcgttg gctgaactta agtgcggccc
     2641 gacctttgac ttgggccagg ccggtgattc ctggtcgcat ctcatggcgt cgggcttgtt
     2701 ccggggtgta gcgggggaga tattcgacga ggagcggtcg tggcccgaca aagctcatat
     2761 cgcccttgag gacattccat aactggggaa gttcatcgag actaaagcgc cgcagccaac
     2821 ggcccagtgg tgtgagacgc tcggcatcag gcagcggttg accatccgcc gaccaggcgt
     2881 ttcgcatggt ccgaaactta taaaggacaa aaagcctgcc tcggtagccc ggccgaacct
     2941 ggcgaaaaat gatcggccgg cccatggtta tccaaacggc tagcgctgtg gcggccatca
     3001 cgggtgaaag gatgatcaat gccacggctg ccccgatgat gtcgattccg cgcttaatcg
     3061 ccagttgcgt ttttcgccat gcggtgacca ttccgcttat tgtcctgttt ttctccaatt
     3121 tgttccgttt ctcgcccagc gcccgagaag atgggcacca gttgtctttc acccagaacg
     3181 ccgcactcaa gcggtttccc caccccgag
//
LOCUS       DAOPTR010000407         3187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_24212_length_3187_cov_3.191890, whole genome shotgun sequence.
ACCESSION   DAOPTR010000407 DAOPTR010000000
VERSION     DAOPTR010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24212_length_3187_cov_3.191890"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            324..674
                     /locus_tag="PKI05_08560"
     CDS             324..674
                     /locus_tag="PKI05_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52296.1"
                     /translation="MSRPDDEAFNLDAILGAGAPEVEGMPGAPPVSPDLAPPAAEAPS
                     EAPAEAKPPKPSLFARLRRIDIFTVMLGLSLLAIIIAVVMLAIELSRYQWDTSARSAR
                     QTVRWVTVTSEHLV"
     gene            complement(836..2098)
                     /locus_tag="PKI05_08565"
     CDS             complement(836..2098)
                     /locus_tag="PKI05_08565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOA52297.1"
                     /translation="MLNSEIVITGIGVVSPIGIGREEFAQSLSHRKSGVGPIGLFDSS
                     GLPVHSAAEVRDFDPRQFIPNRKSVKVMARDSQMGVAASILAYKDARLDSAGVDPERI
                     AVVLGADRICGALEDSKPTYEACLVDGQFHFDRWAPLGMPATFPLVFLKVLPNMIASH
                     ISIAIDARGPNNTIHHGDLSALLAIIEGASLLERGLADVVLVGGASSQMNPFDWARFV
                     SLGRFSRAVDNGRASPRPFDKHRDGEVRGESAAVLVLERVNHAKARGATIWGTIRGWG
                     RCYAPLDGDLNSFPSLSRAISLALQDAAIESKDVAQVNAHGLGTEEDDIRESHGIRRV
                     LSDVPVTAPKSYFGNTGAAGGAVELAASLVLGKDRLVPPTLHYDEADPRCPINVVRGA
                     PQPLASPFLVAVNWTLAGQAAALVVEVA"
BASE COUNT          688 a          906 c          893 g          700 t
ORIGIN      
        1 cggatcggtg cttcttgttc cgtcctcagg ggcaagaata cgaaaaagct gttgatcaga
       61 tcatcgagat gtggcgaaga gcggcaggtg atccgatgag aaaattggtc ggcaaagact
      121 gttcaaattc gcgggaagag ggttaaccag ttcgttttcg cttttccgag gagggtataa
      181 aatcttcttg ggagaaaaaa agttgcagag acccagcgcg gcaatctata ctcgaaagta
      241 ggcctgaacc cgttgcaagg atgtggttgt ccgatcacac gctgcttgtt tgtgtctgcc
      301 tttgagattt aggagggctg gtggtgtccc gtcccgacga tgaagccttt aacttagacg
      361 ccattttagg ggcaggcgcc cctgaggtag aaggcatgcc gggcgccccg ccggtaagcc
      421 cggatttggc tccacccgcg gccgaggctc cttcggaggc tcctgcggag gccaagccgc
      481 ccaagccatc gttgtttgcg cgattgcgca gaatcgacat ttttacggtt atgctcggcc
      541 tgtcgctgtt ggccatcatc attgccgtgg tcatgctggc aattgagttg agccggtatc
      601 aatgggatac ctccgcccgg tcggcccgcc agacagttcg atgggtaacg gtgacttctg
      661 agcaccttgt ctaggtgaag cgaatacttg ttgattgtct gggactaatt ggaatctgct
      721 gcctgctagg tccgcttcgc atgcttcctg tgatcgccct accagcttgg atgggccagc
      781 ttccccctat cgttgaggcg acggtgtcgg acctatcatg cgccgtttgt cagacctacg
      841 ccacttccac aaccagggcg gctgcctgac cggccaaggt ccaattgaca gccaccagaa
      901 aaggcgaagc gagcggctgc ggggcgcctc gaacgacgtt gatagggcat ctcggatcag
      961 cttcatcata gtgcaaggta ggcgggacaa gccggtcttt gccgagaacg aggcttgcag
     1021 ccaattcaac ggcaccacct gccgctccag tgttaccaaa gtaactcttg ggcgcggtga
     1081 ccgggacatc ggagaggacc ctgcgaatcc catggctttc ccggatatca tcttcctcgg
     1141 tccccaatcc gtgagcgttc acctgagcca cgtccttgga ttcaatggca gcatcttgta
     1201 aagccaggga gatggcccgg gaaagtgagg gaaaggaatt caagtcaccg tcgagtggtg
     1261 cgtaacaacg tccccagccg cgaattgttc cccaaatggt ggccccgcgt gcctttgcat
     1321 gatttacgcg ttcgaggacg agaaccgcgg ccgattcccc gcgtacctca ccgtcgcggt
     1381 gtttgtcaaa aggtcgtgga ctcgcccggc cgttgtccac agcccgcgaa aaccgcccca
     1441 gactgacgaa tctcgcccaa tcaaatgggt tcatttggga gcttgcacca cccacgagga
     1501 ccacgtccgc tagcccgcgt tcaagtaatg aggcgccttc gatgatggcc agcaatgccg
     1561 acagatcgcc gtgatgaatc gtgttattgg gcccgcgcgc atcgatcgca attgagatgt
     1621 gcgaggcaat catgttggga aggaccttca gaaacactaa cggaaaggtt gcaggcatac
     1681 cgagcggtgc ccagcgatcg aagtgaaatt gaccatccac caggcatgcc tcgtaggtcg
     1741 gtttgctgtc ctcgagggct ccacatattc ggtcggcccc gaggactacc gcgatccgct
     1801 ccggatccac gccggctgaa tccaacctcg cgtccttgta tgccaaaatg gaggccgcca
     1861 cacccatttg ggagtctcgc gccatcactt tgacactttt gcggttgggg atgaactgtc
     1921 gcggatcgaa gtctcggact tcggcagcgg aatggacagg caagccggac gaatcaaata
     1981 gcccaatcgg tcccaccccg gatttccggt gcgaaagtga ctgcgcaaat tcctcccgtc
     2041 cgataccaat cggactaacg accccgatgc cggtgataac aatttcagaa tttaacattg
     2101 tcaaaaatca acaggaatcg ttttgggaat cctcaatcac gattgtccct acccactaca
     2161 tcggcaccac cggcccacac tcaaagtcac aagttggtgc gcaaatgacg tcactatgcg
     2221 accaccgcaa atgcgtttcg gtaggttacc ctcggaaccc ctcattttta ggccaaaacc
     2281 ttaagtataa ctcaatcacc ccggtgaaca atcgcaccga gctcggtccg agccttttga
     2341 gccttttccg aacttcactt taggccggga ctctcaagac cggcttagga agccggtttg
     2401 ccaggggcga gaactgacgc cttatgggag agaatcttgt cgagctaacg ccggccacgg
     2461 cttgaggccc cgctggcttg cctccgcctg agtcaaagaa tacgaccagt gaaggcggca
     2521 acaaaagcac aaacatggca tttattgcca cgagactagt ttttgttagg cccctcttgt
     2581 gtagcggcca tctaagcgcc tatcccagaa tctcccctct cccgttcgcc gggagagggg
     2641 acgggggtga gggtggctcg aatcgctgcc ggttttctcg atcaactccg acgatcggtt
     2701 gccctcaccc caaccctctc ccagggggag agggggactt tttggaatag acgctaaacg
     2761 tggtaggcac cctaagcgtt tcgagctgtc tggtggggca cagtgttaaa gtttgccaat
     2821 tattacggtc gcggcatttg tgaggccgat aacagttgta ggttctgcaa tgtgacgtac
     2881 tccacctgaa gtaggacggg aacgatgcga acaagcaagg cattcatact gctctctgcc
     2941 atctttggac tggcgaccat tattggttgt cggatgtgtg ccagtcccca cgattcctgc
     3001 ggacccgtgg tctcggcggg atcctctggc cggggttgta cctcagcccg ggcggggtcc
     3061 gccttcgctg ttacggctta ctcggatcat acggacgaaa acagcccggg atatgcttct
     3121 gccccgcacg tggtcgggct tgattccgat cgcagaaccg tatcttcttc gaaaggtccc
     3181 ctaccca
//
LOCUS       DAOPTR010000408         3186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_24223_length_3186_cov_2.941552, whole genome shotgun sequence.
ACCESSION   DAOPTR010000408 DAOPTR010000000
VERSION     DAOPTR010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24223_length_3186_cov_2.941552"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="PKI05_08570"
     CDS             <1..1215
                     /locus_tag="PKI05_08570"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOA52298.1"
                     /translation="SFFASGNNLFVFFIAKDRYDTWQVRGGLLATQRQVGQFLLSLGL
                     QDANREFSLKDLSDTAWQQPAVNLTNELVAGSSADLTADFPELIIVPDGILWYLPFEA
                     LHVKVNGEVLPLIAKHRIRYAVTSSLGVSFQPTSSVPQPRTLIYLGRLNPQEDAAVLE
                     KRAQLLLQSVPGAVVLSTSAVTAPPTLLGKLFDELIVLDDLQGTYTPYAWAPLSWGRT
                     GVTLADWLNLPYRAPQLVILPGYHTPTESALRKSEGTPTGQEIALALTALQGMGSKTL
                     VVSRWRTGGGTMTSILKEFLQEYPQTSAAAAFQRALLLASSEPLDPNDEPRVQKKAPE
                     KQIVKSHPFLWANMFLVDSGSFAEPAENALPKTEQKEPEEPKMPEEKQDNEKAKDDAK
                     ENDAPAAPDAAQ"
     gene            1582..>3186
                     /locus_tag="PKI05_08575"
     CDS             1582..>3186
                     /locus_tag="PKI05_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOA52299.1"
                     /translation="MLRACDRRLFQGWSGYLVFICVSAWLASGVQYRFVFGEESALQS
                     AQENQGADGRSDLDRAVELKLSARTFSDLGEVIRLCEEALKKGLSQEDTEFAKQLLAA
                     TRIQRGMMIGQTLLGGLTVDRNWPAFRTLALEDLEKGLAAAPDQPEAWLVVSQLQSLP
                     GGDREKALAAASKVVELATDNPRLVARALILRAELVEDKEKALADLDKVVELMPDDPD
                     PLRLRGQTKLQVNKFEEALADFDAALKLEPNHVPTLESKIDALLELKRFDDALKVCDE
                     IEKLAPLSSEAIFHRARIAAVQKEFEKALELLNKAAELDPKNPAILMIRAAVYQELKK
                     TEEALKDVNAALALRPGNPALLRLRAALLAGSGKFSEAIADLEQVRKAQSPEDQVLLN
                     LQLGLLYSAQKYYGRAVEHFTKVLEVEPKNVAALRGRGDALLSMGRQAEAIKDYEKAL
                     EVDPKDSGVLNNLAWVLATSPDEKLRDGKRALELAKQACELTDYKEAHILSTLAAAYA
                     EIGDMEKAIEWSRKAVELGEGEEKEAL"
BASE COUNT          740 a          840 c          913 g          693 t
ORIGIN      
        1 agtttttttg cctcgggaaa caatctgttc gttttcttta tcgccaagga ccgttacgac
       61 acctggcaag tgcgcggtgg gttactggcg actcaaagac aggttggtca gttcctactt
      121 tccttggggc tgcaagacgc aaaccgcgag ttttccctca aagatttgtc tgacaccgct
      181 tggcagcaac cggccgtcaa tttgaccaac gagcttgtgg ccggctcaag tgccgatttg
      241 acggcggact ttcccgaatt gattatcgtc ccagatggta ttctctggta tttgcctttc
      301 gaggccctgc atgtcaaggt aaacggcgaa gtcctaccac tgatcgccaa gcaccgcatc
      361 cgatatgctg tcacatcgtc gctgggcgta tcgttccaac cgacctctag tgtcccacag
      421 ccgaggacat tgatctattt ggggcgactc aatcctcagg aagacgccgc cgtattggaa
      481 aagcgagcac aactcctgct tcagagcgtt ccgggagccg tggtcttatc cacgagcgct
      541 gtcacggccc cgcccacatt gcttggcaaa ttgttcgatg agctgattgt gcttgacgat
      601 ttacaaggaa catacacgcc atacgcctgg gcgccactat cttggggacg aacgggggta
      661 acccttgcgg actggttgaa tctgccgtat cgagcacccc aattggttat tctccctggt
      721 taccatacac caacggaaag cgctttgcgg aagagcgaag gaacacctac aggacaagaa
      781 attgcgctcg ccctgaccgc attgcagggc atggggtcaa agacgctggt cgtcagtcgg
      841 tggcgaacgg gcggtggaac catgacatcg attctcaaag agttcctcca ggagtatccc
      901 caaacatctg ccgcggcagc atttcaacgg gcactgcttc tggcatcgag cgaacccctc
      961 gatcccaacg atgaaccgcg agtgcagaaa aaggctcccg aaaagcagat cgttaagagt
     1021 cacccattct tgtgggcaaa catgttcttg gtggattcgg ggtcattcgc cgaacccgca
     1081 gagaatgccc tccccaaaac agaacaaaaa gaacccgagg aacccaagat gccggaagag
     1141 aagcaggata acgagaaagc gaaggacgac gcaaaggaga atgatgctcc ggcagcccca
     1201 gatgctgccc agtagggttg attgcaccgt gcatctctga ggtcgcccgg acctattccg
     1261 ctttcacttc cggtaaactg tgtgatcgta aaccgtgtaa ccggagagca aacccatctt
     1321 gatccacgtt ctgccggtaa cttttttcgt tttcagggca tcttgtggcc ggagaggtac
     1381 cgagtgttcc gaggattttc tgtctcttgg atcgcgagat acttggcgga aggggtttcg
     1441 ttttccgttc ctatagccaa gacttgcgtc attgcgacat aatggggagt aagctgtgcg
     1501 acaaggagaa agtcagtgtt gtttggggat ggtcccgccc atgtgacggc gttttgtcga
     1561 gtaaccattg aggagactga tatgttgcgt gcctgtgatc ggcggttgtt tcaaggttgg
     1621 tcgggatacc ttgttttcat ttgcgtctct gcttggctgg cttctggcgt gcagtatcgg
     1681 ttcgtttttg gagaggaatc cgcccttcag tccgctcaag agaatcaggg cgcggatgga
     1741 cgtagcgatc tcgaccgagc cgttgagctc aagctatcag cccgcacatt cagtgatctc
     1801 ggggaagtca tccggctttg tgaagaggcc ttgaagaagg ggctcagcca ggaagacacc
     1861 gagtttgcaa agcaactttt ggcggcaacg cgtatccagc ggggcatgat gattggccaa
     1921 accctcctcg gcgggctgac cgttgaccgg aattggccag catttcgcac gctagcgctc
     1981 gaagaccttg aaaaaggcct ggccgccgct ccggatcaac cagaggcctg gttggttgtc
     2041 agccagctcc aaagtttgcc cggcggtgac cgggagaagg ccctcgccgc tgcctcgaag
     2101 gtcgtcgaat tggctaccga caatccacgg ctcgtggcac gagcgttaat tcttcgggcg
     2161 gagttggtcg aggataagga gaaggccttg gccgaccttg acaaggtcgt tgagcttatg
     2221 ccggatgacc ccgatcccct gcgattacga ggccaaacca agctgcaagt gaacaagttc
     2281 gaggaggccc tcgccgactt cgatgcagca cttaagctgg aaccaaacca cgtcccgacg
     2341 ctcgaatcca aaattgatgc tcttctcgag cttaagcggt tcgacgacgc cctaaaggtg
     2401 tgtgatgaga tcgaaaagct ggcgcccttg tcgtcggaag caatttttca ccgggcccga
     2461 attgccgcgg tccaaaaaga gtttgaaaag gcgttggaac ttctcaacaa ggcggcggaa
     2521 ctggatccca agaaccccgc catcctgatg atccgtgctg cggtgtatca ggagctcaag
     2581 aaaaccgagg aggcccttaa agatgtcaat gcagctttag ccctccgacc aggaaatccg
     2641 gctctccttc ggttacgtgc cgccctttta gcaggcagcg gtaagttcag cgaagccatt
     2701 gccgatttgg aacaggtgcg aaaggcccaa tcgccggagg atcaggtact cctgaatctc
     2761 cagttgggtc ttctctattc agcccagaag tattacggac gggcagtgga gcatttcacc
     2821 aaagtgctcg aggtcgaacc caagaatgtc gctgcgttgc ggggacgcgg tgacgcactt
     2881 ttaagcatgg gccggcaggc agaagcgatc aaggattacg aaaaggcttt ggaagtcgat
     2941 cccaaagaca gcggtgtcct gaacaatctg gcatgggtgt tggcgacatc gcccgacgaa
     3001 aaactgcggg acggtaaacg ggccctcgag ttggccaaac aggcatgcga attgactgac
     3061 tacaaggagg cacacattct cagcaccttg gcggcggctt acgctgagat cggtgatatg
     3121 gaaaaggcca tcgaatggtc caggaaggct gtcgagcttg gcgaaggtga ggagaaagaa
     3181 gccctt
//
LOCUS       DAOPTR010000409         3183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_24250_length_3183_cov_3.376918, whole genome shotgun sequence.
ACCESSION   DAOPTR010000409 DAOPTR010000000
VERSION     DAOPTR010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24250_length_3183_cov_3.376918"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..557
                     /locus_tag="PKI05_08580"
     CDS             117..557
                     /locus_tag="PKI05_08580"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOA52300.1"
                     /translation="MPRRAARHIPEAEAEMTPMIDMTFQLIAFFMILINFSEGDQNQL
                     IRLPASELAKPPDTAPAWPITLQMTSKGTVFYGVDELTLENVQPVLEIEKELIRRRGG
                     DFRNTTIIIRADRFAKTGRVQELIRICQSTGFEKFTLRAKQESP"
     gene            559..1053
                     /locus_tag="PKI05_08585"
     CDS             559..1053
                     /locus_tag="PKI05_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOA52301.1"
                     /translation="MKFRGSGKHVGGKIELMMTPMIDVVFQLLTFFIMTFKIVAPEGD
                     FFVKMPLSAPSAGVPSDLQLPPIKVRMTAGPGGELAGLYLSERRLANFNELREQIRAL
                     VGDSGGPGSAGDAEVELDCDYDLRYEYVIRAITAVSGYVSPDGQIVRLIEKIKFAPPR
                     SEGI"
     gene            complement(1292..2230)
                     /gene="xerD"
                     /locus_tag="PKI05_08590"
     CDS             complement(1292..2230)
                     /gene="xerD"
                     /locus_tag="PKI05_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOA52302.1"
                     /translation="MSPARRRLKPHVASPTRATAAWEDSFVHYLSAECHLSENTVAAY
                     RRDLRRFLEWLNARDLKGLTVKDLSDYASWLHEKGLAASSLARHIVSLRLFLRYLQLE
                     GVLTTNQAELLASPQVWERIPKVLTPQQVDALLDSPKPFDPCYRRNRAILEFLYATGC
                     RVSEVANLRMEDVHVEERYCLCRGKGNKERVVPLGERAIMRFQEYLEHERPKLAKKNG
                     VDPPWAFLSYRGRRIRRERIWELLKLYALRAGLPGDISPHVLRHSFATHLLAGGADLR
                     LVQEMLGHASIATTQIYTHVDFARLKAIHRRFHPRA"
     gene            complement(2439..3134)
                     /locus_tag="PKI05_08595"
     CDS             complement(2439..3134)
                     /locus_tag="PKI05_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52303.1"
                     /translation="MGFLKKFIGSVLREGSQTPSRSSFEHLSEEELEAHLRIAKYGNF
                     TLTDAVRPSYDLQVVPCEGFRHDVYRSDNGQTVIPVLMASVSKEKLFDTFIELLDPLG
                     EEVDVVLETSHHRDRPGHHDLYREHIDMPVLKSILWEFEDVLLNDGCTGIAVLNADIP
                     LEVQFDEHKLLICYGSDLRPFERILKSHGIPNNEQIRFITEAEHVHSSTEHYSRRFEE
                     LKTYLGMDEGCYV"
BASE COUNT          763 a          892 c          826 g          702 t
ORIGIN      
        1 ctgatgtcac gattctcaag cgtggccccg aagaaatgac cgtcggaaac attaacgcca
       61 ttgatcatcg acagacaaca tttggcgttg ttttctaaat cgcagaggtg cagattatgc
      121 cccgtcgtgc tgcccggcac atacctgagg cggaagctga aatgacgccc atgattgata
      181 tgacgtttca gcttatcgcc ttcttcatga ttctgatcaa cttttcggaa ggcgatcaga
      241 atcagttgat ccgcctgcct gccagtgaac tggccaaacc gccggacaca gctcccgcct
      301 ggcccatcac gctgcaaatg acatcaaagg gcaccgtttt ttacggcgtc gatgagctaa
      361 ccttggaaaa cgtccaacct gtgcttgaga tcgaaaaaga actcatccgc cgtcgcggcg
      421 gtgactttcg aaataccact atcatcattc gcgcggaccg ctttgccaaa accggccggg
      481 ttcaggaatt aatccgcatc tgtcaaagta ccgggtttga aaaattcaca ctccgggcaa
      541 aacaggagag tccatagcat gaagtttcgt gggtctggaa aacacgtggg gggcaaaatc
      601 gagctgatga tgacgccgat gatcgacgtc gtcttccagc ttctcacgtt cttcattatg
      661 acgtttaaaa tcgtggcccc ggaaggagac ttcttcgtta agatgccgct ttcggcaccc
      721 agcgcgggtg ttccgtcgga tttgcagttg cctccgatca aagtccgcat gacggccgga
      781 cctggaggtg aattggccgg actttacctc tccgaacggc gtctcgcaaa ttttaacgaa
      841 ttacgcgagc aaatccgggc cttggtgggc gatagcggtg gcccaggaag tgctggcgat
      901 gccgaagtcg aactggattg cgactacgac ctccggtatg agtacgtgat tcgggcaatc
      961 acagcggtct cgggatatgt tagccccgat ggtcaaatcg tgcggctcat cgagaagatc
     1021 aaatttgccc caccccggtc agagggcatt tagagtctat cctaaaagag tctatcctaa
     1081 aatcccccct ctccctccgg gagagggttg gggtgagggc aagcgcacgt cggaatctat
     1141 cgagaaaacc ggcagtgatt cgagccaccc ttacccctgc cccctctccc gccgaacgcg
     1201 tgaggggaga ttatgggatg ggctcttaaa cattgtgtgt gagcattgcc ccacaagctc
     1261 tcaagcactc gtccacgcca ccggcgacgg ttcaggcccg cggatgaaac cgccgatgga
     1321 tagccttgag tcgtgcgaaa tctacgtgag tatagatttg ggttgtggcg atggaggcgt
     1381 gccccagcat ctcttggaca agccgaaggt cggcgccacc cgccagaaga tgcgttgcaa
     1441 agctatggcg caacacatgc ggactgatgt cgccgggaag ccccgctctc agggcataaa
     1501 gcttcagtag ttcccaaatc ctttcccgcc gaatccgacg tccgcgatac gacaaaaaag
     1561 cccagggcgg gtccacgccg tttttcttcg ccaacttcgg tcgctcgtgc tccaggtact
     1621 cctgaaaacg catgatcgca cgctccccta ggggaacgac tcgctccttg ttacctttgc
     1681 cccgacagag acaatatcgt tcctcgacgt gaacgtcttc catccgcaga ttggccacct
     1741 cgctcacccg acatcccgtg gcgtataaaa actccaagat agcacggtta cgccgataac
     1801 agggatcgaa cggcttggga ctatcgagca aggcatcgac ctgctgagga gttaagacct
     1861 taggaattcg ctcccaaacc tgcggactcg ccagtagctc ggcctgattt gtggttaaaa
     1921 cgccttccag ttgaagatac cgcaggaaca gacgtagtga tacgatgtgc cgcgctaacg
     1981 agctggcagc caatcccttc tcatggagcc aactcgcata atccgacaag tccttgacag
     2041 tcaggccctt gaggtctcgc gcgttaagcc attccaggaa acggcgaaga tcacgacgat
     2101 aggccgcaac tgtgttctct gagagatggc actcggccga caaataatga acaaagctat
     2161 cctcccacgc agcggttgcc ctggtgggag aggcgacatg gggcttcagc cgacgccgtg
     2221 cgggactcat atcctaccca atggttaagg gcaacatttc ttctctatag gcaacttcgg
     2281 caacccggct gtgaaaagtt gattgcgccc gagctccgac atctcaccca aactttcggc
     2341 acagataagg cccaagagta tcggatggaa tggcaatgcc gaagtagtcc ccggctaccc
     2401 gtcagatcgg aatagacatc attcagctgg tcgatctgtc aaacgtagca accttcatcc
     2461 atccctaggt aggttttcag ttcttcaaag cgacgggagt aatgctctgt cgaggaatgg
     2521 acgtgttcgg cttccgtgat gaaacgaatt tgctcattgt tggggattcc gtgacttttc
     2581 agaattctct caaatggccg caaatccgag ccgtaacaga taagcagctt atgttcatca
     2641 aattggacct ccaatgggat atcggcgttt aacacggcaa tgcccgtgca gccatcatta
     2701 agaaggacat cttcgaactc ccagaggatg cttttcaaga cgggcatgtc gatgtgctcc
     2761 cgatagaggt catgatgtcc cggccgatcc cggtgatggc tagtctcaag cactacatcg
     2821 acctcctcac caaggggatc caacagctcg ataaaggtat cgaacagctt ctccttggag
     2881 acagaggcca tcaagaccgg gataaccgtt tgcccgttgt cactgcgata aacgtcgtgg
     2941 cggaaccctt cacaaggaac cacttggaga tcatacgagg gccgtacggc atcagtcagg
     3001 gtaaagtttc catatttggc aattctcaag tgggcctcaa gctcttcctc actgagatgc
     3061 tcgaagctac tccgcgacgg cgtctgagac ccttctcgca acacgctgcc aatgaatttc
     3121 ttgaggaagc ccatcgttgt gccagaccct tttgttgcag ttaccgtgac gtgaccttcc
     3181 cga
//
LOCUS       DAOPTR010000410         3181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_24271_length_3181_cov_3.264875, whole genome shotgun sequence.
ACCESSION   DAOPTR010000410 DAOPTR010000000
VERSION     DAOPTR010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24271_length_3181_cov_3.264875"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /gene="uxaC"
                     /locus_tag="PKI05_08600"
     CDS             complement(<1..1406)
                     /gene="uxaC"
                     /locus_tag="PKI05_08600"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOA52304.1"
                     /translation="MADVFITENFMLENEMAVRLYHEVARDLPIVDYHSHLSPRLIAE
                     DYTFPNLTVLWLRGDHYKWRVMRANGVPEYFCTGNASDYEVFMKWAETVPKLLRNPLY
                     HWTHMELKKPFGISDRLLCPETAADIWRICNEKLTQPEFSCRGLLKQMNVVLVCTTDD
                     PTDDLKYHAQLAAETDFPVRVLPTFRPDRAMILANPESFHLWVDRLSEVSGVEVKDFS
                     TLIEALRKRHDYFHEMGCRVSDHGIEQFYAEDAKESELNQIFRDAYNGRLVSTNERAK
                     FKSAVLYHCAVMNYEKGWTQQFHVGALRNNNTRMFKQVGPDTGFDAINDVSMARNMVR
                     FFDRLDQEGKLTRTIVYDINPAFNEVFASILACFQDGSEPGKMQFGAAWWFLDQRDGI
                     LRQLETVSNFGLLSRFVGMLTDSRSFLSFVRHDYFRRILCNLLGSEMERGLLPKDFDL
                     VAKLVRDVCYNNAVRYFG"
     gene            complement(1617..2492)
                     /locus_tag="PKI05_08605"
     CDS             complement(1617..2492)
                     /locus_tag="PKI05_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52305.1"
                     /translation="MESTQQGSERDIILFTRPWVVCFDPRRVDEGWTLRYCPDFSDMP
                     MQSQVEDVSPVATIFALHHPASRGSWRPPLIDVTTRSTDVCCRYEKTEDFAAEITLWM
                     RMATRLPGGEQHQAGSQDGNSLLRGGRISLALQTEDDIDPRLLFVHNVHQAVEKEECE
                     IVISPDDLLRRSLLPGVFFDAQKEDMGTSRLTVTRYRCAASDWSLILYFYPAGVRRIS
                     PLCFEGRAAQNNQVLPQSCEEVWAITTVSGRLEKGVTAVFHGGFLLVPPSMRPSELSQ
                     QIRDLVLNESPLFEG"
     gene            complement(2583..>3181)
                     /locus_tag="PKI05_08610"
     CDS             complement(2583..>3181)
                     /locus_tag="PKI05_08610"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA52306.1"
                     /translation="EKTSGHGHLVDWGIHLIDATRMILGLTTPQKVVAAGGIYQLKGI
                     ITTPDTLTVHFEFDRCPVVWRHRLWGAEEYSPDVNNGIFFYGTKATVFVTDGRWVVVP
                     AQKNSERKEYTPKTDAGVEHMRNFLTAVRERKQPACPIEEGYRSTATVKLAMIAYESD
                     SIVRWDESQGDIQDNPTARALLKREYRAPWRHPADQEA"
BASE COUNT          759 a          927 c          784 g          711 t
ORIGIN      
        1 aatccaaagt atcgcacggc gttattgtag caaacgtctc gtacgagttt tgccacaaga
       61 tcgaagtctt tgggtaacaa gccgcgttcc atttcactcc ccaagagatt gcaaaggatc
      121 cttcggaaat agtcgtggcg gacaaacgag aggaacgacc ggctgtcggt cagcatcccc
      181 acgaagcggc tgagcaggcc gaaattggaa accgtctcta attggcggag aataccatct
      241 cgctgatcaa gaaaccacca tgccgcccca aattgcatct ttcccggctc agaaccgtcc
      301 tgaaagcacg ccaaaatgct ggcgaagacc tcattgaaag ccggattgat atcatagaca
      361 atcgtccttg tcagtttccc ttcctgatcg agacgatcga agaaccgcac catatttcgg
      421 gccatgctga cgtcattgat ggcgtcaaac ccggtatccg gtccaacctg tttgaacatc
      481 cgtgtgttgt tgttccgcaa ggccccgacg tggaattgct gcgtccaccc cttctcgtag
      541 ttcatgacgg cacaatgata cagcacggcc gacttgaatt tggcccgctc attcgtgctg
      601 accaagcggc cgttataagc gtcacgaaag atttgattca actcggactc tttcgcatct
      661 tcggcataaa actgctcaat gccgtgatcc gaaacacgac aacccatctc gtggaaataa
      721 tcatgccgct tacggagggc ttcgatcagc gtggaaaaat ccttcacctc cacgccggag
      781 acttcactca accgatcaac ccacaaatgg aaactctccg ggttggccaa gatcatcgcc
      841 cggtccggtc gaaatgtcgg cagcacgcgg accggaaaat ccgtctcggc cgctaattgg
      901 gcatggtact tgagatcatc cgtcggatca tcagtcgtgc agaccagcac aacattcatc
      961 tgcttgagaa ggccccgaca ggaaaactca ggctgagtta gcttttcatt acaaatccgc
     1021 cagatatcgg cggccgtctc cgggcagaga agccgatcgc ttattccgaa gggctttttc
     1081 agctccatgt gggtccaatg gtaaagcgga ttgcggagca gttttgggac ggtctctgcc
     1141 cacttcatga acacctcata atcgcttgca ttgcccgtgc aaaagtattc ggggacgccg
     1201 tttgcccgca tgacgcgcca cttgtagtgg tcaccacgaa gccatagcac tgtcaaatta
     1261 gggaatgtgt aatcctcggc aatcaaccgc ggtgacaagt gggaatgata gtccacaatc
     1321 ggaaggtccc gcgcaacttc atggtaaagc ctgacggcca tttcgttttc cagcatgaag
     1381 ttttcggtta tgaacacgtc agccacatcg ttctccttct accaaggaaa ctcaaacctg
     1441 ataccgttaa tttcctgctt gccccgagac ttcagatttg cactcccacc ccctaaagct
     1501 gcccaccgat tgccagcaag cagcaagtat ccacccaatc agcaaaacgg ctgtgcgttc
     1561 tctcgacgca aaacaatgcg atcccatgct accgggagat gccgagaatt tccaggttat
     1621 ccctcaaaca gtggggattc atttagcaca agatcgcgga tttgctggga aagctctgat
     1681 ggtcgcattg acggtggcac caaaagaaag ccaccgtgaa aaactgctgt gacacctttt
     1741 tcgagccgcc ccgaaacggt cgtgatagcc cagacctctt cgcacgattg cggcagtact
     1801 tggttgtttt gggcagcacg accttcgaaa caaagcggcg atatcctccg cacgcccgcg
     1861 gggtaaaaat agaggatcag tgaccagtcc gaggccgcac aacggtatcg cgtgacagtc
     1921 aatctagacg tgcccatgtc ctctttctgc gcgtcaaaaa acacccctgg caagagtgaa
     1981 cgccgcagaa ggtcatctgg cgagataacg atctcacatt cctctttttc cacagcttga
     2041 tgaacgttgt ggacaaatag aagacgaggg tcgatgtcgt cttcggtctg caacgccaga
     2101 ctgatgcggc ctcctctcag aagggaattt ccatcctggc ttcccgcttg gtgttgctca
     2161 ccccctggca gccgagtagc catcctcatc catagcgtta tctcggccgc aaaatcctcg
     2221 gtcttttcgt aacggcaaca tacatctgtg ctccttgtcg tgacatctat taacggtggg
     2281 cgccaagatc cgcgtgacgc cggatggtga agggcaaaga tggtcgctac tggagaaaca
     2341 tcctcgacct ggctctgcat cggcatgtcc gagaaatcgg gacaataccg cagcgtccat
     2401 ccttcatcca cacggcgggg atcgaaacac acgacccacg gccgcgtaaa gaggataata
     2461 tcccgctcgc tgccttgctg ggtgctttcc atgaccttta tccctcctgg tcaacgagac
     2521 gactcgagag cgcccgcctc gaagtgcggg ttactctgac tgcgaagaat cgtgggccaa
     2581 gactacgcct cttggtcggc aggatgtcgc caaggggcgc ggtattcccg tttcagcaac
     2641 gcccgagcag ttggattatc ctgaatatcc ccttgcgact catcccaacg cacaatgctg
     2701 tccgactcgt aagcaatcat cgccaacttg accgtggccg tcgagcggta gccttcttca
     2761 atggggcagg ccggctgctt ccgttctcgg acggcggtta ggaagttccg catatgttca
     2821 acgccggcgt cggtcttcgg cgtatattct ttacgctcgc tgttcttctg cgccgggacg
     2881 accacccacc gaccgtcggt tacaaatacc gtggccttgg ttccgtaaaa gaaaatccca
     2941 ttattgacat cgggtgagta ctcctcggcc ccccacaatc ggtgccgcca aaccacgggg
     3001 catcgatcaa attcgaaatg aacggtcaag gtgtctggag tggtgataat ccccttgagc
     3061 tgataaatcc ctccagctgc cactaccttt tgaggtgtcg ttagccccaa gatcatccgc
     3121 gtggcatcga tcaggtgaat cccccaatca acaagatggc catgccctga tgtcttttcc
     3181 a
//
LOCUS       DAOPTR010000411         3181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_24272_length_3181_cov_3.206654, whole genome shotgun sequence.
ACCESSION   DAOPTR010000411 DAOPTR010000000
VERSION     DAOPTR010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24272_length_3181_cov_3.206654"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1481)
                     /gene="argS"
                     /locus_tag="PKI05_08615"
     CDS             complement(<1..1481)
                     /gene="argS"
                     /locus_tag="PKI05_08615"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOA52307.1"
                     /translation="MNVLSELRKRFSAILTDYVESPEEFLKLIHPSQDDRFGDYQADL
                     VMPLAKRLGRKPRELAEELVNRLDVSDMAHPPEIAGPGFINFRLRDEYLEQLLERLVQ
                     DPRLGVPLAEKPETIIIDYSAPNVAKPMHVGHIRSTVIGDALYRTLRFLGHRVISDNH
                     IGDWGTQFGMIIYGYRHFLNAEAYQQNPVQELARLYRLVRQLIDYHESVQKQAEIKRQ
                     LEEKEAALADLRNQLQNMTEAERKKITRKLRQLESEVAQLQDDLSEIRTKIEAVECDE
                     TLRRAAMEHTDIGQKVLQETAKLHAGDPENLKLWREFLPHSLDEMNRIYKRLGIQFDF
                     TLGESFYHDRLGALVDQLLKLGIAQESEGAICIFFPDIEAPMIIRKQDGAFLYATTDL
                     ATLCYRMETWKPDTILYVVDFRQSLHFQQLFAVARKLGFDQVKLVHVSFGTVLGEDGR
                     PFRTREGDTVGLESLLDEAVRRAAEIVNANDDAKSNGPELSPE"
     gene            complement(1583..2002)
                     /gene="rsfS"
                     /locus_tag="PKI05_08620"
     CDS             complement(1583..2002)
                     /gene="rsfS"
                     /locus_tag="PKI05_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOA52308.1"
                     /translation="MNESENLSPALRDKLSSLEKALLAARTADEHRGQDIVILDMREL
                     TTFFDFFVIATGTSRRQLHAMSEHIDEALAQCGARRLGIEGFGESRWILLDYGDVVVH
                     LFDEETREFYALEDLWAHAGRIPFTPRSSLPDAGNGV"
     gene            2285..2920
                     /locus_tag="PKI05_08625"
     CDS             2285..2920
                     /locus_tag="PKI05_08625"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOA52309.1"
                     /translation="MMSRYDNVYVLDVMSEDRPGIVAAVSAAVEELGGNIDACSQTVL
                     GGYFTLIMIVSLPQRMSPAELAERVRQEGGNWDLQVLARAASPSLPSLTKPAHDRFVI
                     TAFGTDQPGIVRRFSQYLASKDINIVDLYGDRSGNEFVLIGQLEVPTKWDIRLLQADL
                     EQMGKDLGFTVKLQHENVFVATNQLRLFHASERWPTLGGTAQVPAQTAKTR"
BASE COUNT          747 a          871 c          852 g          711 t
ORIGIN      
        1 tcctcgggag aaagctccgg accattggac ttcgcatcgt cgttagcgtt gacgatttcg
       61 gccgcacggc ggacggcctc gtcaaggaga gactccagtc caaccgtgtc accctcccgc
      121 gtgcgaaacg gccgtccatc ctcacctaag acggtgccaa aactgacgtg cacaagtttc
      181 acctggtcga accctagttt ccttgccacg gcaaataatt gctgaaaatg gagactttgg
      241 cggaagtcca caacatagag gatggtatcc ggcttccatg tttccatacg atagcaaagc
      301 gtggccaagt cggtcgttgc gtaaaggaag gccccgtcct gtttgcggat gatcatgggg
      361 gcttcaatat cggggaagaa aatacagata gccccctcac tttcttgggc aataccaagt
      421 ttgagtaact gatcgaccag ggccccaagc cgatcatggt aaaaactttc gccaagcgtg
      481 aaatcgaact gaatcccgag ccgcttatag atgcgattca tttcatcgag gctgtgtggc
      541 aggaattccc gccataactt gaggttttcg ggatcgcccg catggagctt ggccgtctct
      601 tgaagcacct tctgcccgat gtccgtatgt tccatcgccg cgcggcgcaa ggtctcgtcg
      661 cactccacgg cctcaatctt ggtgcgaatc tcactcagat catcttgtaa ctgagcaacc
      721 tccgactcga gctgccggag tttcctcgtg atcttctttc gttctgcctc cgtcatgttc
      781 tgcaactgat ttcggagatc agcgagagca gcctcttttt cctcaagctg ccgtttgatt
      841 tctgcctgct tttgaacact ttcgtgataa tcgatcaact ggcggacgag gcgatagagg
      901 cgtgccagtt cctgcaccgg gttttgctga taggcttcgg cattcaagaa atgacggtat
      961 ccgtaaatga tcattccgaa ctgtgtgccc caatctccga tgtgattgtc actgatgacg
     1021 cgatgcccaa gaaagcgaag tgttcggtag agtgcgtccc cgatgaccgt ggaacgaata
     1081 tgaccgacgt gcatgggctt ggccacgtta ggagccgagt aatcaatgat gatggtctct
     1141 ggcttctcgg ccaggggaac gcccagcctg ggatcctgta caagtctttc gaggagttgc
     1201 tccaaatact catctcgaag ccgaaaatta atgaagcccg gcccggcaat ttccggtgga
     1261 tgggccatgt ctgagacatc caaacggtta accagttcct ccgccagttc ccggggcttt
     1321 cggcccaatc gcttcgctag cggcatgact aggtcggcct ggtaatcgcc aaagcgatca
     1381 tcttgactgg ggtgaatcag tttcaggaac tcctctgggg attccacgta gtccgtaaga
     1441 atggccgaaa aacgcttccg taattcactc aatacgttca tcgactaatc cttatctcgg
     1501 ttgtatcctc caagaacgat cctaagagac aaagcaagtg tgacacgcag gggatgagaa
     1561 tggcaaggac cctgagggcc ggtcaaacgc cgttccccgc gtctgggagc gaagatctgg
     1621 gagtgaaggg gatccgcccg gcgtgtgccc acaagtcttc caaggcgtaa aattcccgtg
     1681 tctcttcatc aaagaggtga acaacgacat cgccgtaatc caggaggatc cagcggcttt
     1741 caccgaaccc ctcgattccc aatcgccgcg caccgcactg agcgagggcc tcgtcaatgt
     1801 gttcgctcat ggcgtgaagc tgacggcggc tcgtcccggt agctatcaca aaaaagtcaa
     1861 aaaacgtagt taattcccgc atgtccaaaa tgacaatatc ctgcccacgg tgttcgtcgg
     1921 ccgttcgggc ggccaaaagg gccttttcca gggaggacag tttgtctcgc agggctggtg
     1981 acaggttttc tgattcgttc acgtatccac tctccatagc caaaaaggtg gaaccgctcc
     2041 agttatttta gttagaatcc ctccgggcgc gaacaactcg acagccgcaa tcgtctgacg
     2101 tagcatagtc ccaatttgtg taaaataggg gtaagaagct catgtggctc cacaacaggg
     2161 cccattatta tgttatgccc atgcttgcca tcagagggct tccatattta acgagtaaac
     2221 gtgactaccg ttcccgccgc cgataacggg gcgaagcaga ttctgttcga gaggacaaga
     2281 gcctatgatg agtcgatatg acaacgtgta tgtcttggac gtcatgtcgg aagaccgacc
     2341 cggcatcgtg gccgccgtca gcgcggccgt cgaggaattg gggggcaata tcgacgcgtg
     2401 cagtcagacc gtgcttggcg ggtactttac cctcattatg attgtcagcc tgccccaacg
     2461 gatgtcgccg gcggaactgg cagaacgtgt gcgacaggaa ggcgggaact gggaccttca
     2521 ggtcctggcc cgcgcggcat cgccatcatt gccttctttg accaaaccag cacacgaccg
     2581 atttgtaatc acggccttcg gaacggacca gcccggcatt gtcaggcggt ttagtcaata
     2641 cctcgccagc aaagacatca atatcgtaga cttgtacggt gatcgctccg gaaacgagtt
     2701 cgtcctgatt gggcaacttg aggtccctac aaaatgggat attcggctcc tccaggcgga
     2761 cttggaacaa atgggaaagg acctgggctt tacggtcaag ctgcagcacg aaaatgtctt
     2821 cgtcgctacc aaccagttgc ggctgtttca tgccagtgaa cgatggccga ctcttggtgg
     2881 taccgcacaa gtccctgcac agacggcaaa gacacggtga gacgctaacg ggtacacgag
     2941 aaaagggctc tccaggacgt gagatttgac cgcggcgcaa aaacgcggcc gttaagaact
     3001 aattacaggg gctggaaaaa gaaagaggcg actacttgta agtagtcgcc tcataaagtt
     3061 tgctgttgtg ccggaactgt gcccacctat cggttcaaga gtcgctccga accacttccg
     3121 gcgcagtgac catcagagca acctggcaag ccgctccacc agatctaccg ttcggcaact
     3181 g
//
LOCUS       DAOPTR010000412         3172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_24350_length_3172_cov_3.659609, whole genome shotgun sequence.
ACCESSION   DAOPTR010000412 DAOPTR010000000
VERSION     DAOPTR010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24350_length_3172_cov_3.659609"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PKI05_08630"
     CDS             complement(<1..664)
                     /locus_tag="PKI05_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOA52310.1"
                     /translation="MTTLDIWPDDHPRDFGTETTCAGHPTLRGTVIVVSLLIALAIGE
                     GCRSMLELPDRAKQSGYDPQEPAEEGWLFRRLLGQQEGSEHNSPSASQTKTPPAASSP
                     GNKGSSSSETQLTSGTLPEDNANSLSQLPPVDQDTVIVPPVQSEEEETEDFILFELPS
                     RFFKMFKPKQDESLARSYYEKALDLYRQRRYEEAAPLFKKAARHWQRTILEEDALFLA
                     GES"
     gene            complement(636..1430)
                     /gene="recO"
                     /locus_tag="PKI05_08635"
     CDS             complement(636..1430)
                     /gene="recO"
                     /locus_tag="PKI05_08635"
                     /inference="COORDINATES: protein motif:HMM:NF014607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOA52311.1"
                     /translation="MSAEQATALVLRTYDFSESSLVVTLFTREFGKIRALAKGAKRPK
                     NPFDFALDLLALCRIVFLHKSTEALHLLTEAKLLRRFRVERSGLEGWYAALYASDLVN
                     AMTHDEDAQPMLFDLLEDSLNRFQTRTRLKDHLLRFEWRLLSLTGHGPSLDACVSCGE
                     EIKNIAATTQNSDDTQRKTSRSEVFFALTEGGVLCPRCRAGKRAVVSVHAEALATLRL
                     LGEDSDKEVHIPPSIYGELRGLLNQYLCHLLGQRPRMHDYLGYLAG"
     gene            complement(1445..1849)
                     /locus_tag="PKI05_08640"
     CDS             complement(1445..1849)
                     /locus_tag="PKI05_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOA52312.1"
                     /translation="MPVQMQLSRIIMSEISDEQYIFLKEVDGNRTFPIVIGAFEAQSI
                     YRAVRNVSLPRPMTHDLIINIFHELQVEPQDILINELRNHTYFARLRVRHNGSLVEID
                     ARPSDAIAVAVRYNPPLPIYVAEEVLAEALGG"
     gene            complement(2073..2783)
                     /locus_tag="PKI05_08645"
     CDS             complement(2073..2783)
                     /locus_tag="PKI05_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HOA52313.1"
                     /translation="MTEVQKVQPAFRSERIVPRRRARIGWYPGADYQIIQAQAVLSLL
                     FGVLSILCFLTVYVFWIPLAGLGFGWVARKEIAKAPKERLGLGMAKTGMLLSLVFGIT
                     GCTWVMYWSLAAAPPGYKLITFAELQPNPDEPKLIPQFARDLEGKKVFIWGYMIPGEK
                     QYGITDFVMVGQVSHCQFCQAQLLPTQMIEVHLTHGIKLDYTTKKIGVGGVFTCSPDA
                     LKQQFGGIVYRIEADVVR"
     gene            complement(3054..>3172)
                     /locus_tag="PKI05_08650"
     CDS             complement(3054..>3172)
                     /locus_tag="PKI05_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA52314.1"
                     /translation="PASVPLAFGIAVAVGIIFGLYPARRAANMDPIEALRHE"
BASE COUNT          712 a          892 c          872 g          696 t
ORIGIN      
        1 aggattctcc cgccagaaag agggcatctt cttcgaggat ggtccgttgc caatggcgag
       61 ccgctttttt gaagagggga gccgcctctt cgtaccgccg ctgccggtat agatcgagcg
      121 ccttctcgta gtaagaccga gccagggact cgtcctgctt gggcttgaac atcttgaaga
      181 accggctggg aagttcaaag aggatgaagt cctccgtctc ttcttcctcg ctttgaaccg
      241 gcggcacaat cacggtgtcc tgatcgaccg ggggaagctg cgagagagaa ttagcattat
      301 cttccggaag ggtcccagag gttaactgag tttctgatga agaagaccct ttgttcccgg
      361 gggaactggc tgccggagga gtctttgtct gggacgcgga gggagagttg tgctctgatc
      421 cctcctgctg tcctaggaga cggcgaaaaa gccaaccttc ttcggccggc tcctgcggat
      481 cgtatccgct ttgtttggcg cggtcgggga gctccaacat gctacggcat ccctcgccga
      541 tcgccagggc aatcaatagg ctcacgacga tgacggtacc ccgaagggtt gggtgtcccg
      601 cgcacgtcgt ttccgtgccg aagtctcgcg ggtgatcatc cggccaaata tccaaggtag
      661 tcatgcatcc gtggcctttg gcccaaaaga tgacagagat actggtttag caatcctcgt
      721 aattctccat aaatcgatgg cggaatatgc acttctttgt cggagtcttc ccccaatagc
      781 cgcagtgtgg caagcgcctc ggcgtgaacc gagacgactg cccgcttgcc ggctcggcat
      841 cggggacaga gcacgcctcc ctctgtcaag gcgaagaaca cctccgaccg ggaagtcttt
      901 ctttgcgtat catcgctgtt ttgagtcgta gccgcgatgt tcttgatctc ttcaccacaa
      961 ctcacgcacg catccaacga ggggccgtgt cccgtcagcg acagcaatcg ccactcaaat
     1021 cgcagcagat gatctttcag tcttgttcgc gtttgaaagc ggtttaggga atcctccaaa
     1081 aggtcaaaca gcatgggctg agcgtcctca tcgtgggtca tggcattgac cagatcagac
     1141 gcatataacg ccgcatacca gccttccagc ccactccgtt ctacccgaaa gcgacgcaat
     1201 agcttcgcct cggtgaggag atggagggcc tcggtcgatt tgtggaggaa cactatccga
     1261 caaagggcga ggaggtcaag ggcaaagtcg aaggggttct tgggtctttt agctcctttg
     1321 gccaaagccc gtattttgcc aaattcccgc gtaaacaggg tgaccaccaa actgctttcg
     1381 ctgaagtcgt atgttcgcag gaccaaggcg gttgcttgtt cggcactcat gggggcggtc
     1441 ccacttagcc gccgagggcc tcggccagga cctcttctgc gacgtagatt ggaagcggag
     1501 gattgtaccg aactgccacg gcaatggcat cagaaggtcg ggcgtcaatc tccaccaagg
     1561 agccgttgtg gcggacgcgt agcctggcga aatacgtatg attgcggagc tcgttgatca
     1621 agatgtcttg cggctcgacc tgcaattcgt ggaagatatt gataatcaaa tcgtgcgtca
     1681 tcggcctggg gaggctcaca ttacggacgg cccgatagat gctctgcgct tcaaatgccc
     1741 caatgacgat aggaaaagtc cgattcccat ccacctcttt gaggaagatg tattgctcgt
     1801 cgctgatttc gctcatgata attcgggaaa gctgcatctg gacgggcatc gagtgcctcc
     1861 gctgtcctgc aaacgaccgc acatgccttc ggggacgtct gttgcgctga actttgcttt
     1921 caaccccagt tttccacctt acttttttat tctatcgaag gaacggtatc gctcaccaga
     1981 cgcgggttgg aggatcggcg aaagtttgtc tcggcttgtg gtgctgccaa ttcttgccgc
     2041 acgatgggca cctacgcagt ataggcaaac ctttaccgaa ccacgtctgc ctcaatgcga
     2101 tagacgatcc ctccgaattg ctgcttgaga gcgtctggac tacaggtaaa aacaccgccc
     2161 acgccgatct tcttcgtcgt gtagtccagt ttgatgccgt gcgtcaagtg gacttcgatc
     2221 atctgggtag gaagtaattg ggcctgacaa aactgacagt gcgatacttg tcccaccatc
     2281 acgaaatctg taatgccgta ttgcttttcc ccggggatca tgtatcccca gataaagacc
     2341 ttcttacctt ctaggtcccg ggcgaattgg ggaatcagct tgggctcgtc agggttcggc
     2401 tgcagctcgg caaaggtgat caatttgtat cccggcgggg ccgccgccaa agaccaatac
     2461 atgacccagg tgcagcctgt gatcccaaac accaacgaca aaagcatccc cgtcttggcc
     2521 atccccagtc cgagacgttc tttgggtgct ttggcgatct cttttcgtgc cacccaaccg
     2581 aaacccagtc cggccagggg aatccaaaaa acatagacag tcagaaaaca gaggatagaa
     2641 aggacgccga agaggaggct caacacggcc tgggcttgaa tgatctgata gtccgcccca
     2701 gggtaccacc cgatccgcgc acgacggcgt ggaacgattc gctcacttcg aaacgcaggc
     2761 tggacttttt gcacttcggt catgacgatg aatcccgaaa aacctcgcat tccgcggaaa
     2821 tacgctcgtg tattccgacg gagcttaaac ctccgcgggt agcatggcca ttaagaacct
     2881 cgccttatgg cactggacag cgaaaacgtg cttcccgggg ggaataaacc caccataaaa
     2941 cctgatcgca acgggccacc gtagtctttc acctttcccc attattgccg attcgcggaa
     3001 agagttcgag gaaattgcaa tggggcaagc ggaaagaccc acggacggcg ccatcactcg
     3061 tggcgcaggg cctcaatggg atccatattt gccgcacgtc gggcaggata cagcccgaag
     3121 atgatcccca ccgccactgc aatcccaaac gccaggggca cggacgccgg aa
//
LOCUS       DAOPTR010000413         3153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_24541_length_3153_cov_3.258877, whole genome shotgun sequence.
ACCESSION   DAOPTR010000413 DAOPTR010000000
VERSION     DAOPTR010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24541_length_3153_cov_3.258877"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PKI05_08655"
     CDS             complement(<1..649)
                     /locus_tag="PKI05_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA52315.1"
                     /translation="MAKELRIGMLGCGFMGRAHSNAWLQVSHFFDREYKPVLKACYGR
                     EEDRQQLETFAKRWGYESIETDWRRLIERPDIDLVDVCVPNHMHYDCVIAAAQAGKII
                     VCEKPLAMNAQQAEEMVAAVEKAGVPNMVSFNYRRVPAIALAKQIIEEGRIGRPFHYR
                     ATYNQDYTISTKVPQGGLALWRLDARIAGSGVTGDLLAHSIDTAEWLNGPIKKVVA"
     gene            complement(719..1789)
                     /locus_tag="PKI05_08660"
     CDS             complement(719..1789)
                     /locus_tag="PKI05_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009095387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOA52316.1"
                     /translation="MATRNNFPKLHNAAWPGVVGKGDGGEPPIDLDTMLDLTAAAEVD
                     GIKFEGFDLFLYLPHIDIDSTVKSDDAIKQLADKATKRNLKIGTVVAPIWPPTGGGSA
                     MGDESERRKFLDQVRKGCRVAMVLRELGVRPYGCVRIDSACSVSDWLSDPEANQRRIA
                     ETFRQACDIAADHGEKLAAEGEICWGGMHSWRRMIQLLEMVDRPNVLGFQADMAHTLL
                     YLLGYNAPEDAILPQDFDWKDSERFYAAYKQLTDALRPWTIDLHIAQNDATVHGSGSH
                     DKTGRHCLATDPNGKLDIPRAAGFWLRDENGNVTKRMRHICWDGCMFPNAVMMNPQTW
                     NDILRVMIQVRDQHGWYEEESA"
     gene            2100..>3153
                     /locus_tag="PKI05_08665"
     CDS             2100..>3153
                     /locus_tag="PKI05_08665"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOA52317.1"
                     /translation="MKRRSGFTLVEILVATAISLIMLGAVVAVFASVMQSINQARAVL
                     ETSDRLRSAAELLRADLKGLTVIPVPPRDPANNEGYFEYTEGPIGQNGQIRPFAGAHP
                     LAVVNLDDLDAAGNPYQDSTVGDNDDILMFTTRSTGRPFVGRCLVSGQPTTIESDVAE
                     VAWFVRGNRLYRRLRIVAPKAMALCDVNANGRLDPAEWIPANDTGAVNFNRYYDVAVH
                     FDRNAGGWVFSTLGDLTKPENRYAHLIGDPLNPASFPFHPHRAGWQPLGLPTLAESAS
                     AGAATTWLTRGLLPALSLTSLAAPLDKWDLWTNPYPVAEVDPQTGLLRTVGQLYRESE
                     DLVLTNVVAFDVKIWDP"
BASE COUNT          690 a          904 c          828 g          731 t
ORIGIN      
        1 aggcaacaac cttcttgatt gggccgttga gccactcagc cgtatcgatc gagtgtgcaa
       61 gcaagtcgcc ggtcacgccc gacccagcaa tgcgagcatc gagtcgccaa agggccaaac
      121 cgccctgcgg gacctttgtg gagatcgtgt aatcctggtt ataggtcgcc cgatagtgga
      181 aaggccgccc gatacggcct tcttcaataa tttgcttcgc caaggcaatc gctgggacgc
      241 ggcggtaatt gaaggagacc atatttggga cgccagcctt ctcaacggcc gcgaccatct
      301 cctcggcttg ctgggcattc atagccagtg gcttctcaca gacgataatc ttaccagcct
      361 gggccgccgc tataacgcag tcatagtgca tgtggttggg tacgcacaca tcgacgaggt
      421 caatgtcggg tcgttctatc aaccgccgcc agtcggtttc gatcgactcg tagccccagc
      481 gtttggcaaa ggtctctaat tgctgacggt cttcctcccg gccatagcag gctttcaaaa
      541 cgggcttgta ttctcgatca aagaaatggg atacctggag ccaggcgttc gaatgagccc
      601 tgcccatgaa cccacaaccc aacatgccga tccgaagttc ctttgccatg attcgttctc
      661 ctttaccaag tgactcgtga tataccgata tgcccaacaa aaatccggta aaacaacatc
      721 acgcggattc ttcttcatac caaccgtgct gatcgcgcac ctggatcatc acccgcaaga
      781 tgtcattcca ggtttgagga ttcatcataa ccgcattggg aaacatacaa ccatcccagc
      841 agatatgtcg catccgtttc gttacattac cgttttcatc tcttagccag aaccctgctg
      901 cccgggggat gtccaatttt ccattgggat cagtcgccaa acaatgccga ccggtcttat
      961 cgtgtgaacc cgatccatgg accgtcgcat cattttgggc aatatgtagg tcgattgtcc
     1021 aggggcgcag cgcatccgtt aactgcttat acgcggcgta aaaccgttcc gaatccttcc
     1081 agtcgaaatc ttggggcagg atagcgtctt cgggcgcgtt gtaaccgaga aggtacaaca
     1141 gtgtgtgggc catatcggcc tgaaagccca gcacgttggg acgatccacc atttccagaa
     1201 gctggatcat ccgacgccaa ctgtgcatgc caccccaaca gatttcgccc tctgcggcca
     1261 gtttttcgcc atgatcggcc gcgatatcgc aggcctggcg aaatgtctcc gcaatccgcc
     1321 gctgattggc ctcggggtca ctcagccaat cgctgacact gcaagccgaa tcaatccgca
     1381 cacacccgta agggcgaacg cccagctctc gcaagaccat ggccacccga caacctttgc
     1441 ggacctgatc gaggaacttt cgacgttcgc tctcgtcccc catggcggaa ccaccacctg
     1501 tcgggggcca aataggtgca acgacagttc cgatcttcag gtttcgtttt gtcgccttgt
     1561 ccgccagttg cttgatggca tcatcgctct tgaccgtgga gtcgatatcg atatgcggta
     1621 aatagaggaa gagatcgaat ccctcaaact tgatcccatc gacctccgcg gctgccgtca
     1681 agtccagcat cgtgtcgaga tcgatgggag gttcgccacc atcgcccttt ccaacgaccc
     1741 ctggccaggc tgcgttgtgc agtttaggaa agttgttacg cgttgccatc gctcgccgtc
     1801 ctttctttgc tccaggtaat caccgggatc tctcatgacc atcggcgtac tttccttttg
     1861 gtcaagtcgc gtacgattct aagccttcct acgaaaggtt acaagtcacc gcaattgact
     1921 tttgcgattc cagagtgtcg aatactaata aggggaaggc aaagccgctg aagatggttt
     1981 ccgggctttt ccaacgggcc agcacgactc ttgagtgata ggagacatac cacatggctc
     2041 aaggtttgat aggcccgtta cacacagcgt ccctaaatct caaccggcga ggctctttca
     2101 tgaaaagacg atccggtttc accctcgtag aaatcttggt tgccacagcg atttcgctca
     2161 ttatgctagg agctgtggta gcggtctttg cctcggtcat gcaaagtatc aatcaagcga
     2221 gggctgttct ggaaacgtca gatcggctgc gatcggcggc ggagctgctt cgggcggacc
     2281 tcaaagggtt gaccgtgatc cccgtgccac cgcgcgaccc ggcaaataac gaggggtatt
     2341 tcgaatacac ggaagggccg attgggcaaa atggtcagat ccgaccattt gcgggggccc
     2401 atcccttggc cgttgtgaat cttgatgact tggacgccgc cggaaacccc taccaagatt
     2461 cgaccgttgg cgataacgat gatattctca tgttcaccac gcgttccacc ggccgaccat
     2521 ttgtggggcg ttgtttggtg agtggccagc ccacgactat tgaatcggat gtcgccgaag
     2581 ttgcctggtt cgtccgcgga aaccgtttgt atcgccggtt gcgaattgtc gcccctaaag
     2641 cgatggcgct atgtgatgtc aacgcaaatg gacgtcttga tcccgcagaa tggatccctg
     2701 ctaacgatac cggagccgtg aatttcaacc gctattacga tgtggcggtc catttcgaca
     2761 ggaatgccgg cggttgggtc ttttccacgc tgggcgatct cacaaaaccc gagaatcgtt
     2821 acgcccactt gataggtgat ccattgaatc ctgcaagctt tccattccat ccccaccggg
     2881 cgggatggca gcccttgggt ttgccaacgt tagcggaaag tgcttccgca ggggcggcga
     2941 cgacgtggct aacgaggggc ctattgccgg ctctcagctt aacatcattg gcggcacctc
     3001 tggataagtg ggacctctgg acgaatccct atcccgttgc ggaggtggat ccccagacag
     3061 ggcttttgcg aacggtcggg caactttacc gcgagtcgga agaccttgtc ttaacgaacg
     3121 tcgtggcttt tgacgtgaag atctgggatc cca
//
LOCUS       DAOPTR010000414         3147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_24601_length_3147_cov_3.221216, whole genome shotgun sequence.
ACCESSION   DAOPTR010000414 DAOPTR010000000
VERSION     DAOPTR010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24601_length_3147_cov_3.221216"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..1368)
                     /locus_tag="PKI05_08670"
     CDS             complement(118..1368)
                     /locus_tag="PKI05_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOA52318.1"
                     /translation="MKYVYRDRFTRQLIYAEKQTNIGIAGPGTIDGQGTNEAFDARAN
                     PGDRDRPYILRFAECTGVRVREVTLLESARWCAHFLGCDDVLIDGVTILSSKRENRDG
                     LDIDSCHRVRVANCRVETGDDAIVLKATAHRPCKNVTVVNCHLSSQAAAFKLGTESNG
                     GFEDIVMSNCTIYDTGGAGISLLMVDGGVLDRVSISNVVMDNVASPIFIRLGNRARPL
                     PDEEPPGQGMMRNIVISGIQARGAGVLGCAISGIPGACIEHLTLRDIRIQSAGGGTVE
                     NGRATVPEREAAYPSPRIFGPLPAYGLFIRHVRHLTLDGVELSFDTARGEERPALVLE
                     DVHDAYLTKLRAAAVSAMPTVRMVNCQGVLLHGCRAEQVSTPFLSVEGESTRDIAVVG
                     NDFSGTQTAMIVDPLVPESAVRHR"
     gene            complement(1365..1688)
                     /locus_tag="PKI05_08675"
     CDS             complement(1365..1688)
                     /locus_tag="PKI05_08675"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOA52319.1"
                     /translation="MIRHTWVAFLLVVVNTANLGARECLPNVFDVRDFGAVGDGITLD
                     TKAIQAAIDHCAAEGGGRVLLTGKRVYLSGTVVLKSGVTLWIDSGTVLRASLDKDDYP
                     DLRRS"
     gene            complement(1730..>3147)
                     /locus_tag="PKI05_08680"
     CDS             complement(1730..>3147)
                     /locus_tag="PKI05_08680"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 140 protein"
                     /protein_id="HOA52320.1"
                     /translation="LGWLLADAIGVLCFALPRATGLEPLRVADNQRFLMTAGGKPFFW
                     LADTAWQLIHDLDEAEMRRYFADRREKGFTVIQTVVLVELRSDKPNAFGHFPIEPRRP
                     DRPIVEPGPNNDYWDDVERVLRLAEENNLYVALLPTWGKWVISNWQDGIVDGFFTTEN
                     AEIYGRFIGNRFRDHNNIIWVLGGDKASPTDEAKAIWRAMARGIAIGVTGEEDYSKVL
                     MTYHTSGPGSTAWFLNKEPWLDIHGLQSGHGRWALNWMMVEYAYTMRPTLPVIDLETS
                     YPGFRHGRPPTVATDDDARRAAYWAVFAGAAGHTYGHHSIWQMHSPKYPGVAGPKEYW
                     YEALDAPSAFQMGYLRRLIESRPFLTQRPDLSLLAFEQTKPWEMCLALRGDGYALVYT
                     PTGRTLEIALDKLGFANVKAAWFDPRTGNTSEIGQFPARGRRSFDPPGEPGEGNDWVL
                     ILDDAARTFPISDLKPGPKKP"
BASE COUNT          721 a          964 c          821 g          641 t
ORIGIN      
        1 cataaaacaa tcggccacga tggctgtgca cgcactgtcg ctggcatacc aaaccaagcg
       61 cttcgctcaa gcaaagaaaa gagtctatcg tccgcgcccc ttgttcatct ttcgcggcta
      121 ccggtgtcga acggcggact ccggaacaag cggatcgacg atcatcgccg tttgcgtgcc
      181 ggaaaagtcg ttaccaacaa cggcaatgtc gcgagttgat tcaccttcga cgcttaaaaa
      241 cggagtacta acttgctcgg cgcgacaacc atgcaagagg accccctggc aattgaccat
      301 ccggactgtt ggcattgcgc tgaccgcagc agcacggagc tttgtcaaat acgcatcatg
      361 cacatcctcg aggaccaatg ccggtcgctc ttcaccccgg gcagtgtcaa agctcagctc
      421 cacgccatca agtgtcagat ggcggacatg gcgaataaac agcccatacg ctggcaaagg
      481 accgaaaatc cgaggcgacg gataagcggc ctcgcgttca ggaacagtgg cccggccgtt
      541 ttcaaccgtt ccaccacctg cggactggat acggatgtcg cgcagggtta ggtgctcaat
      601 acaagcccca ggaatacccg atatggcaca gccgagcaca ccggcgccac gggcttggat
      661 tccgctgatc acaatattcc gcatcatgcc ttgccccggc ggttcttcat cgggcaaagg
      721 tcgggcacga ttgccgagac ggataaagat gggcgacgca acgttgtcca tcaccacatt
      781 cgagatcgac accctatcta gcactccccc gtcgaccatt agcaaggaga taccggctcc
      841 gcccgtgtca tagattgtgc aattcgacat gacgatatcc tcgaagcccc cattcgactc
      901 cgtgcccagc ttgaaggcgg cagcttggct tgacaggtgg caattaacga cggtcacatt
      961 tttgcacggg cggtgggccg tggccttcaa gacgatcgcg tcgtcgcctg tttcaacccg
     1021 gcaattcgcc acacgcaccc gatggcaact gtcgatgtcc agtccgtcgc gattctcacg
     1081 cttgctggaa aggatggtga caccatcgat caaaacatca tcgcatccca agaaatgggc
     1141 acaccaacgg gccgattcaa gcagcgtaac ctcacgcacg cgaactcccg tgcactcggc
     1201 aaaacgaagg atgtaagggc ggtctcggtc gcccgggttc gcccgcgcat caaaggcttc
     1261 attggtcccc tggccgtcaa ttgttcccgg tcccgcaatc ccaatgttcg tttgtttctc
     1321 cgcgtaaatg agttgccgag taaaccgatc ccggtataca tatttcaact gcggcgtaag
     1381 tcgggatagt catctttatc gagactggcg cgcaacacgg tgccgctgtc gatccacaaa
     1441 gtgacaccac ttttcaatac caccgttccc gataaataaa cccgttttcc cgttagcagg
     1501 acgcgtccac caccctcggc cgcgcaatgg tcgatagccg cttgaattgc ttttgtgtcc
     1561 agcgtgatac catcacccac ggcaccaaag tcacggacat caaagacgtt aggaagacac
     1621 tcgcgtgcac caaggtttgc cgtattcacg acaacgagaa ggaacgcgac ccacgtgtga
     1681 cgaatcatgg tacaccaccc gtgacgattg gcggaagcta agaaaggtct cacggttttt
     1741 tcggtccagg ttttaggtcc gagatgggga aggtccgtgc tgcatcgtcc aggatcagca
     1801 cccaatcgtt gccctcgcct ggctctcctg gtgggtcgaa cgaccggcga ccgcgggccg
     1861 gaaattgccc tatctcgctc gtgttgcctg tgcggggatc aaaccacgca gctttgacat
     1921 tcgcaaagcc gagcttgtcc agggcaattt ccagggttcg accggtcggt gtgtacacga
     1981 gggcgtagcc atccccgcgt aacgccaaac acatttccca aggcttcgtc tgttcaaagg
     2041 cgagcaagga aaggtcaggc cgttgtgtga gaaatggtcg ggactcgatc aaccgccgca
     2101 agtatcccat ttggaatgcg cttggggcat cgagggcttc gtaccaatac tccttgggtc
     2161 cggcgacacc tggatatttg ggcgagtgca tttgccaaat actatggtgg ccgtacgtgt
     2221 gcccggccgc gcccgcgaaa accgcccaat aagcggcccg ccgcgcgtca tcgtcggtgg
     2281 ccaccgtggg ggggcgacca tgtcgaaaac ccgggtaact tgtttccaaa tcaatcaccg
     2341 gcaacgtggg tctcatggtg taggcgtact cgaccatcat ccaattcagc gcccagcggc
     2401 catgcccgga ttgcaaccca tggatgtcaa gccacggttc tttgttcaaa aaccaggccg
     2461 tgctacctgg accactggtg tgatatgtca tcaacacttt cgagtagtct tcctcacccg
     2521 tcacaccgat ggcgataccc cgggccatgg cccgccagat cgccttggct tcgtccgtgg
     2581 gcgaggcctt atcgcccccc aaaacccaaa tgatgttgtt gtgatcgcgg aatcgattgc
     2641 caataaaccg gccataaatt tctgcgttct ccgtcgtgaa aaacccatcg acgatgccat
     2701 cctgccaatt ggaaatgacc catttacccc acgttggcag cagagcgaca tacaggttgt
     2761 tctcctctgc cagccgcaga acgcgctcta cgtcatccca ataatcgttg ttcggccctg
     2821 gttcgacaat cggtcgatcc ggtcgtctcg gttcaatagg aaaatgccca aaagcgttgg
     2881 gcttatcgct ccgcaactct acgagcacca cggtttggat caccgtaaaa cccttttccc
     2941 ggcggtcggc gaagtagcgc cgcatttcgg cctcatccaa gtcgtggatc agttgccatg
     3001 ccgtgtccgc cagccaaaag aagggcttac caccggcagt catcagaaac cgctggttat
     3061 cggcaacgcg caatggttct aacccagtcg ccctgggcaa cgcgaagcac aaaacgccaa
     3121 tagcgtcagc taataaccac ccaagcg
//
LOCUS       DAOPTR010000415         3141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_24655_length_3141_cov_3.670123, whole genome shotgun sequence.
ACCESSION   DAOPTR010000415 DAOPTR010000000
VERSION     DAOPTR010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24655_length_3141_cov_3.670123"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="PKI05_08685"
     CDS             <1..1125
                     /locus_tag="PKI05_08685"
                     /inference="COORDINATES: protein motif:HMM:NF039904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOA52321.1"
                     /translation="VVTHPDNVTEKMKDRLRPLTDSDKLRYWRRTLRMAALCHDIGHL
                     PFSHAAEKELLPEEWDHERLTRELIASEEMQEIWNRITPPLRVEDVVKLAVGPKKAKH
                     LTFNDWETILSEIIVGDAFGVDRMDYLLRDSHHIGVAYGKFDHYRLIDTLRILPMPSC
                     GDDNESSEELALGVEEGGIHSAEALLLARYFMYSQVYCHPVRRIYDIHLTDFLREWLP
                     DARFPTDLQQHLRMTDNEVTAALLDAAHDQKKRGHVHARRIVARDHFKLIYQRNPSDV
                     EINPEAGKAIFEALCDKFGSENFRHDRYHQKSGSSDFPVKMRDGRIVSSLAISQALNT
                     IPVVSVDYVFAERAVFNEAEKYLRQHRDKIIRPKKEEDNG"
     gene            1118..1666
                     /locus_tag="PKI05_08690"
     CDS             1118..1666
                     /locus_tag="PKI05_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52322.1"
                     /translation="MDRLKKAALLTRLIKRLREEESWCGETHVQKATFFLQRLMQVPL
                     GFDFILYKHGPFSFDLRDELTALRADDLVRLEPQGPYGPRIAATERSKYIEELYSKTL
                     AEYGGRIDFVAKSLGRKGVADLERLATALYITELAGSGASVNERANQMTALKPHIPPE
                     IARDAIEQVDRIIREAQRYLPA"
     gene            1663..2970
                     /locus_tag="PKI05_08695"
     CDS             1663..2970
                     /locus_tag="PKI05_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HOA52323.1"
                     /translation="MRGTDVVNDRRLIEDYLPIEAISREASREKSVRKGHISTLHLWW
                     ARRPLVACRAAVYAALVPADRWVKEVNLKNPPPHPEKAERMKNGAKLGLNRGAAAEFV
                     TRLCKYPGDPKVIAEAQRHILEAHAERLSVEIGKPVTVEDIVEGRAPRPKVLDMFAGG
                     GAIPLEALRLGCEAYALDLNPVAHIIELCTLVYPQKYGKPDPNTRGMTRPGQAGGLSS
                     TWGGLAKEVRYWGNWVLQKVKAEIGDLYPLIPERGRTGVPPVPPVPPVIQGELFQTRR
                     HLPHWQIGGSVYFITFSTKKIELPEVCRMTILDACRFWDGKKIRLHLVCVMPDHVHAL
                     IEPLEETPGVYHDLSKVLHSIKSFTAHEINKMMRRSGPLWEEESWDRIIRDEKDYYEK
                     WQYIVNNPVKAGLSASPEQYAFLYANEKLAWEITGPAGTGDNT"
BASE COUNT          771 a          835 c          880 g          655 t
ORIGIN      
        1 gttgtcacgc acccagacaa cgttacagaa aaaatgaaag accgtcttcg gccgctcact
       61 gattctgata agttgcgata ctggcgccgt acgcttcgta tggccgccct atgtcacgac
      121 attggccact tgccgttttc ccatgctgcg gaaaaagagc tgctccccga agaatgggat
      181 cacgaaagac ttacgcggga gcttatcgct agcgaagaga tgcaggagat ttggaatagg
      241 attacgccac ctcttcgagt tgaagatgtg gtgaaacttg cggtgggacc caagaaagca
      301 aaacacctta cgttcaatga ttgggagacc attctttcgg aaatcatcgt cggtgatgcg
      361 tttggggtcg atcgcatgga ctatctcttg cgggattccc accatattgg cgtcgcatac
      421 ggcaagttcg accattatcg gctgattgac accctccgta ttcttcccat gccatcatgc
      481 ggagacgaca atgaaagctc agaggaattg gctcttgggg ttgaagaagg cggaattcat
      541 tccgctgagg ccttattgct cgcccgttat ttcatgtatt cccaggtcta ctgtcatcca
      601 gttcgtagaa tctatgacat ccatttgacc gacttcctta gggaatggct tcctgatgcc
      661 cgattcccca ccgatctaca gcaacactta agaatgacag acaacgaagt caccgctgcg
      721 ctattggatg ccgcgcatga ccaaaagaaa cggggacacg tacatgcgcg tcggatcgtt
      781 gcacgcgatc atttcaagct tatctatcag cgaaacccca gcgatgtcga aataaatccg
      841 gaggcaggaa aagccatctt cgaagcttta tgcgacaaat ttggaagcga gaatttccgg
      901 cacgaccgat atcaccagaa aagcggcagt tctgatttcc cggtaaagat gcgcgacggg
      961 cgaattgttt catcgttggc catctcgcaa gcgctgaaca cgatacccgt cgtttccgtc
     1021 gactacgtgt tcgccgaacg agcggtattc aacgaagcgg aaaagtatct gagacaacat
     1081 cgagacaaga tcatacgacc gaagaaggag gaagacaatg gataggctaa agaaagccgc
     1141 gttgcttacg cgcttaatta agaggctgcg cgaggaggaa agctggtgtg gcgaaaccca
     1201 tgtccagaag gccacgttct tcttacagcg tctcatgcaa gttcctttgg ggttcgattt
     1261 cattctgtac aagcatgggc ccttctcgtt tgacctgaga gacgaactca ccgcattgcg
     1321 tgcggatgat ttggtgaggc ttgaaccgca ggggccttat ggcccgcgga ttgcagccac
     1381 ggagcgcagc aaatatatcg aggagctcta ttctaagacc ctggcggagt atggcggccg
     1441 catcgatttc gtggcgaaga gcctgggccg gaagggagtc gcggatctcg aacgattggc
     1501 taccgcactc tacatcactg agcttgcggg cagtggcgct tcggtaaacg agagggccaa
     1561 tcagatgacg gctctcaagc cacacatacc accagagatc gccagagacg caatcgagca
     1621 ggttgatcga atcattaggg aagcccagag gtacttgccc gcatgagagg gaccgatgtt
     1681 gtgaacgacc gccgtctcat tgaagactac ctgcccatcg aggccattag cagagaggcc
     1741 tcgcgggaga agtccgtccg caaggggcac atctcgacgc tgcacctctg gtgggcgcgg
     1801 cgaccgctcg tggcctgccg ggcagcggtg tacgcggccc tggtgccggc cgatcgctgg
     1861 gtcaaggagg tgaacctcaa gaacccgcca ccccacccag agaaggccga gcggatgaag
     1921 aacggtgcca agctgggcct caatcgcggg gcagcggctg agttcgtcac gcgcttgtgc
     1981 aagtatccag gcgacccgaa ggtgatcgcc gaggcgcagc gacacatcct ggaggcccac
     2041 gcggaacggc tttcggtgga aatcggcaaa ccggtcacgg tggaggacat cgtcgagggg
     2101 cgcgcgcccc ggcccaaggt gctcgacatg ttcgccggag gcggtgccat cccgctggag
     2161 gcgctgcggc tcggctgcga ggcctacgcc ctcgacctca accccgtggc ccacatcatc
     2221 gagctctgca cgctcgtcta tccccagaag tacggcaagc ccgacccgaa cacgagaggt
     2281 atgacccggc caggacaggc cggaggcctg tcatccacct ggggtggctt ggccaaggaa
     2341 gtccgctact ggggcaactg ggtgctgcaa aaggtgaagg cagagatagg cgatctctac
     2401 ccgctgattc ctgaaagagg gaggacaggc gtcccgcctg tcccgcctgt cccgcctgtc
     2461 attcagggag agttgttcca gacacgcaga catcttcctc actggcaaat cgggggttcc
     2521 gtttatttca ttaccttttc aacgaagaaa atcgaattgc cagaagtctg ccgaatgaca
     2581 atcctggatg cctgtcgctt ctgggacggt aaaaaaatca gactccacct cgtatgtgtc
     2641 atgccggatc atgtccatgc cctgattgaa cctttagagg aaacgcccgg cgtttaccat
     2701 gacctctcca aagttcttca cagcatcaag agctttactg cccatgaaat aaacaaaatg
     2761 atgcgccgtt ccggcccgtt gtgggaagaa gagagttggg atcggattat acgagacgag
     2821 aaagattatt acgagaaatg gcaatacatt gtgaataatc cggtcaaggc cggattatct
     2881 gctagtccgg aacaatatgc cttcctctat gcaaacgaaa agcttgcctg ggaaataaca
     2941 gggccagctg ggacaggcga caatacttag gaacgtgagc agcttccatg aatgatggga
     3001 taatttgggc cttgaactgg gggttattgg ggtaatccat gccgcaaata ttcgacaata
     3061 ttgacaacta ccttttgtcc gcgataaccc aagctatggg gctgtcccaa tgcgcagatt
     3121 tttgcgtcgg atatttcaac c
//
LOCUS       DAOPTR010000416         3136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_24709_length_3136_cov_3.556962, whole genome shotgun sequence.
ACCESSION   DAOPTR010000416 DAOPTR010000000
VERSION     DAOPTR010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24709_length_3136_cov_3.556962"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2685)
                     /locus_tag="PKI05_08700"
     CDS             complement(<1..2685)
                     /locus_tag="PKI05_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52324.1"
                     /translation="MKALGLHKRTQDIMGFLAPRKTGEPVPTVVPFLLALLMMLPQLT
                     GAIWAEIGTQSPGSSGQSTGVEVDQGGSTPRQATEVVGWQLTPTDTLRKQNLQQQVRQ
                     MAKRLVAERLNEQILQLEENGLTNLPLYSELREMQSHLDALVETHMKEVLLSLEQLIQ
                     APPEKQPALFQIAREKSRNVVVQLLIERQKILRRLRIAEIEERVIRLIERETSILEKT
                     ERSLAESAVRQAASAVALKEDQQDIYTLFRGMKQSLEEIRTWGGRVGAEATAALQSLT
                     ESRVDGLFEQSIQQLSENQFAQAAQVERQIITSLEALLERVRRFQGEMESDSRDAARR
                     MIEALAERQAELWEKTLNAEANIQEVQALTENQLQLQKEIEHAAKKLPQGQESLNQAA
                     EAARQATETLFQGQTQQATEHQQSVIDHLAEALTELQRESPAAEFNALPNSEAVRSQL
                     ADLRQAKAELQQIREEQATVSQTARQGELEEAAKGESRLSQKINDVPTKKSLPEDVTE
                     AIRKAAETVGQSAQALSELSKTQTKPSGQEGQGEFSNQAHPDHPPEAGKDDQNTTASS
                     GENRPQVEKPPKAEGSHEDHAVRMATRRAEQAIDEAISAVEMAIADAERARLAAELVD
                     AVQSMVGSEGQTPDRVNLARQRAAELAQLTQQQLDQVRSTKKAVEAELPLARDPASQA
                     LQQLEKVASQAIEAAALQQEAMGREDLAKQIRSAGDMKKAIALAKEAAEHSYESNSSP
                     AKNQSIPLQEKVTEAAMEALAELARLPSLPEDVNPRGRDAARAALDSAKSASQEAAKR
                     LATPPNGENDGAADLQRKAASNLATAAEQIHGLMESLASHVADKFGQHAQKAENLSEM
                     AVPVHPEATSNLHMAENAAHQGSTEAGQRLGAVTQGHQG"
     gene            complement(2682..>3136)
                     /locus_tag="PKI05_08705"
     CDS             complement(2682..>3136)
                     /locus_tag="PKI05_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52325.1"
                     /translation="DYPPQIAVRTVTRLVLPTAQPTIFVEARDDLGLREVRALGKVVQ
                     ADGEEGGQYEWVLWQGNGPPTRTLNEKYKVDLSQLKAKKGDKIELTVVAEDERGSDRP
                     GAVTQADAIFLEVTDLAGILAVMAEADRESAAQLQEMIDRQLDVGGEQ"
BASE COUNT          524 a          916 c          860 g          836 t
ORIGIN      
        1 cccctgatgt ccttgagtca ctgcaccgag acgttgtccc gcttcagtgc tgccctgatg
       61 agcggcgttt tccgccatgt ggagattgga ggtcgcctcg ggatgaacgg gcaccgccat
      121 ttcggacaga ttctctgcct tttgcgcatg ttgcccaaat ttgtcggcca cgtgggaagc
      181 caacgactcc atcaaaccat ggatttgttc ggcggctgtt gccagatttg acgctgcctt
      241 tctttgcaag tctgccgccc cgtcattctc cccattcggc ggtgttgcga gacgttttgc
      301 cgcttcctgg gacgcgctct tggccgagtc aagtgctgcc ctggccgcat cacgcccgcg
      361 gggattcaca tcctcgggga gactgggcaa tcgagccaac tccgccaggg cttccatcgc
      421 ggcctcagtc accttctctt gaaggggaat actttggttc ttcgcaggtg atgagttgga
      481 ttcgtatgaa tgttctgcgg cctccttggc caaagcaatg gcctttttca tgtcaccggc
      541 cgaacggatt tgtttagcga ggtcctcacg tcccatggcc tcttgttgca gggccgcggc
      601 ttcgatggcc tgtgatgcaa ccttttccag ttgttgaagg gcctgacttg ccggatcacg
      661 ggccaacggt agttccgcct caacagcctt cttggtcgat cgaacttggt cgagctgttg
      721 ctgagtcagt tgtgccaatt ctgctgcccg ttgccgggca agatttactc gatcgggggt
      781 ctgtccctcg ctgccgacca tggattgcac cgcatcaact agttccgcgg ccaagcgtgc
      841 ccgttctgcg tcggctatcg ccatttcaac ggcagaaatg gcctcgtcga tagcctgttc
      901 tgcccggcga gtagccatgc gaaccgcgtg atcctcgtgc gatccctctg ctttgggcgg
      961 cttctcgacc tgtgggcgat tctctccaga cgaggctgtc gtgttttgat catccttgcc
     1021 cgcctctggg ggatgatccg gatgtgcctg attggaaaat tccccctggc cctcctggcc
     1081 acttggcttc gtttgggtct tggacagctc ggatagtgcc tgagccgatt gtcccacggt
     1141 ctcggcggct ttcctgatcg cctcggtaac atcctcgggc agcgattttt tcgtcggaac
     1201 atcattgatt ttctgactca gccggctctc cccctttgca gcctcctcca gttcgccttg
     1261 ccgcgccgtc tgactgaccg tggcttgttc ttcacggatt tgctggagtt cagccttggc
     1321 ctgccgtaaa tcggccaatt gggaacggac cgcttcggaa tttggcaggg cgttaaactc
     1381 ggccgcgggt gattcccgtt gaagctccgt tagggcttct gccaggtgat caatgaccga
     1441 ttgttgatgc tcagtagcct gttgcgtttg accttgaaag agcgtttcag tcgcttgacg
     1501 tgccgcttcg gcagcctggt ttaatgattc ctgtccttgc ggcagcttct tggcggcgtg
     1561 ttcaatctcc ttttgcaact ggagttgatt ctcggtcaag gcttgaacct cttgtatgtt
     1621 ggcttccgca tttagggtct tttcccacag ttcggcttgg cgctctgcca aagcctcaat
     1681 cattcgccgc gccgcgtcgc gggaatcact ttccatttcg ccctggaagc ggcgaactcg
     1741 ctccaggaga gcctccagcg aggtaataat ctggcgctcg acttgagcgg cttgcgcgaa
     1801 ttggttctcc gagagctgtt gaatcgattg ctcaaaaaga ccgtcaacgc gagattctgt
     1861 aagtgactgg agagcggctg tggcttccgc accgacccgt ccaccccatg ttcggatttc
     1921 ctccagagat tgtttcatgc cgcgaaaaag cgtgtaaata tcctgttgat cttccttgag
     1981 tgctacggct gaggcggctt gtcgcacggc actttcggcc agtgaccttt cggttttttc
     2041 caaaatactt gtctcccgtt caatcaaccg tatgactcgc tcctcaattt cggcgatccg
     2101 caggcggcgc aagattttct gccgctcaat cagtaactga accactacat tgcgactctt
     2161 ctctctggcg atctggaaaa gcgcgggctg tttttcgggc ggtgcttgaa taagttgttc
     2221 cagagacagt aggacttcct tcatatgcgt ctcaaccagc gcgtcgagat gcgactgcat
     2281 ttcccgcagc tcggaatata gcggcaaatt tgtcagccca ttttcctcga gttgcagaat
     2341 ttgctcgttg agacgttcgg caacaagccg tttggccatc tggcggacct gttgctggag
     2401 gttctgcttt cggagagtgt ctgtgggtgt caactgccac ccgaccactt ctgttgcctg
     2461 tcggggggtg ctcccgcctt gatccacttc cacccctgtc gattgtccgg acgacccagg
     2521 gctttgcgtc ccgatttctg cccagatggc tccggtgagt tggggcaaca tcataaggag
     2581 ggcaagtaga aacgggacaa ccgtcggaac tggctccccc gtcttgcgtg gtgctaggaa
     2641 gcccatgata tcctgggtcc gtttgtgtaa gcccaaggct ttcattgttc ccctccaacg
     2701 tcaagctggc gatcgatcat ctcttggagc tgagccgcgg attctcgatc ggcctcggcc
     2761 atcacggcca aaataccggc caggtctgtt acttctagga agatggcgtc ggcttgggtc
     2821 acggcgcccg gccggtcgct tccacgttca tcttcagcca ccacggtcag ttcgatttta
     2881 tcaccttttt tggcttttaa ctgacttaaa tcgactttat atttctcgtt gagcgttcgc
     2941 gtgggcgggc cgttcccctg ccacaggacc cactcatact gtcctccttc ctcgccatcc
     3001 gcttgaacga ccttgccaag cgcgcggact tctcgcaggc caagatcgtc ccgcgcttcc
     3061 acaaagatgg ttggttgtgc ggtgggcaaa acaagccgag tgacggtacg gactgcaatc
     3121 tggggtggat aatcgg
//
LOCUS       DAOPTR010000417         3133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_24746_length_3133_cov_3.320338, whole genome shotgun sequence.
ACCESSION   DAOPTR010000417 DAOPTR010000000
VERSION     DAOPTR010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24746_length_3133_cov_3.320338"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            578..2650
                     /locus_tag="PKI05_08710"
     CDS             578..2650
                     /locus_tag="PKI05_08710"
                     /inference="COORDINATES: protein motif:HMM:NF022752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HOA52326.1"
                     /translation="MFVRWYGPALVVLGFVWWASSLASPASEGGTPPGPATHIVEDGG
                     QWVLENAILKVTLNPQTGAWEVLDKRVGHTWASFVEDPKRQNVLRNIKTLDDETLSCE
                     TTIFWRDNKPIAGTVTLRLPRDSADLFMTVDMPDRDVAFSNQRFWTPLFYHSPDGAIA
                     IADYCNGHLYPVAEEQLPRRWFNTSRLDMPWVGVCDVKTGIGYMIIAETSDDGYLELR
                     KVEKDGQSAWVPWIGWSPSMGKFSYPRKLIWHFSDQGSYVALAKRYRTYAQQLGLIVP
                     FSEKLKANPELRRLFGAPDVWGNANLQFAQEAKNAGIRRLLVQGRMPPDQMKQVNELG
                     YLTSEYDNYTDVLPVENEDQIDRSHDLVPQNVVLKSDGERMKAWLTFDKKTQYMKRCP
                     ALWLRTAQRDIPKISSTYPFLGRFIDVTTAEDLYECYDPAHMLTKAQKRQCGVDLLSY
                     VRSLKLVVGGEHGIWWAVPYVDYFEGVMSCNAYFSWPAGHLIPPKSKDEEFTSPWGNK
                     LPPWSEYERWGIGHRERAPLWELVFHDCVVTTWYWGDSSDFLLQAAPEITPKKDAFNV
                     LYGTIPLLWADRRKGSWLNNREVFLRTYRNTCILHEVIAGTEMLNHEFLTDSRDVQRT
                     TFSDGTVAIVNFGSQPYELHVGDKSYLLPQNGFYVKGPRIEQIRELVNGRVVTTINSQ
                     DYQYREEE"
     gene            complement(2882..>3133)
                     /locus_tag="PKI05_08715"
     CDS             complement(2882..>3133)
                     /locus_tag="PKI05_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52327.1"
                     /translation="APAPQPTAPSSSGGTLPMAPTDGQNVSPPAAPAPPPEKAPAELP
                     TSPAPPQLPPAPTTRWGDQDVPPMVVPTVGELPEINISG"
BASE COUNT          646 a          859 c          930 g          698 t
ORIGIN      
        1 tgtcttcctt tttgcctgaa taagaatttg ccttgccagt ttcgaaccta tcccaaagtt
       61 ccctctcccc ctgtgagatg gtggggtgag ggcaagcgac cgtcggagtc aatcgaaaaa
      121 accggcagtg attcgagcca ccctcacccc cggcctctct cccgccgagc gggagagggg
      181 aggttgtagg ataatctctt cgtcaacctt caacgattcg gaatcatgtg ttccccttgc
      241 ttccgggcgg gggactgaaa cgtcgcgcgt cgaaaaaacc cttacgggtt acgggcgttg
      301 atagttctaa ccttgaagat gtgagagata gtcacgtcaa atctgggcag cagctaacgg
      361 tcgctctgtg taaacgtggt acccggttgc cgacacagac cggataaggt atcacagtgt
      421 tcagccctac gggcgttgtg tgacaattgg ccgaggtgtt gccggccgct tagtgcttgc
      481 aaaacctgcc tgatgatcca ccgagttcga tggttgcttc ccttgacgcg atgtgaggtg
      541 tgccatggta tttcgcgctt gctctcggca gaaacccatg tttgtgcgtt ggtatggacc
      601 ggcgttggtt gtgttggggt tcgtctggtg ggcatcctca ttagcctctc ccgcttccga
      661 aggggggacg cccccgggac cggccacgca cattgtcgag gacggtggcc aatgggtcct
      721 ggagaatgcg attctcaagg taaccctcaa tccgcaaacc ggcgcttggg aggtgctcga
      781 caagcgagtt ggccacactt gggcgtcgtt tgttgaggac cccaaacggc aaaacgtcct
      841 tcggaacatc aagacactcg atgacgaaac gctctcctgt gaaaccacca ttttttggcg
      901 ggataacaag ccaattgccg ggaccgtgac gcttcggctt ccccgcgatt cggccgatct
      961 gttcatgacg gtcgatatgc cggaccgcga cgtggcattt tccaaccagc gattctggac
     1021 gcccctcttt tatcacagtc ccgacggggc gattgcgatt gccgattact gcaatggtca
     1081 cctctatccg gtggccgagg agcaactccc ccgccggtgg ttcaacacgt cgcggttgga
     1141 tatgccctgg gtgggtgttt gcgacgtcaa gactgggatt gggtacatga tcatagcgga
     1201 gaccagtgac gacggttatc tggagttgcg caaggtggag aaagacggcc aatccgcttg
     1261 ggtaccttgg attggctggt cccccagcat gggcaagttt tcttatcccc gcaagctgat
     1321 ttggcacttc tcggatcaag ggagttatgt ggccctggcc aagcgctatc ggacgtatgc
     1381 ccaacaactg ggcctcattg taccgttctc agaaaagctc aaagcgaatc ccgagttgag
     1441 gcgtctcttc ggcgcccccg atgtgtgggg gaacgccaac ctgcaatttg cccaggaggc
     1501 gaaaaacgcc ggcatccgcc ggttacttgt gcaaggtcga atgccgcctg accaaatgaa
     1561 acaggtcaac gagttggggt atctgacgag cgaatacgat aactacaccg atgtgttgcc
     1621 cgtagagaac gaggatcaga tcgaccgatc tcacgatctt gtcccgcaaa atgtcgtcct
     1681 caagtccgac ggagagcgga tgaaggcctg gctgaccttc gacaagaaaa ctcagtacat
     1741 gaagcggtgt cccgcgctat ggctgcgcac cgcgcagcgg gacatcccca aaatctcgtc
     1801 cacttatccc ttcctgggcc gcttcattga tgtcaccacc gccgaggacc tttatgagtg
     1861 ctacgatccc gcgcacatgc tcaccaaggc ccaaaaacgc caatgcggtg tggatctgct
     1921 gagttatgtc cgttctctga agctcgtggt gggcggggaa catggtatct ggtgggcggt
     1981 gccctatgtc gattacttcg agggcgtcat gagctgcaac gcctatttct cctggcccgc
     2041 cgggcatctc attccaccga agagcaaaga tgaggaattc actagtccct ggggcaacaa
     2101 gctaccgccg tggagtgagt atgagcgatg ggggattggc catcgagagc gggcaccgct
     2161 ttgggagctt gtcttccatg actgcgtcgt cacgacctgg tactggggtg attcgagcga
     2221 ttttctgctg caagcggccc cggaaatcac ccccaagaaa gatgccttca acgtgttgta
     2281 cggtaccatc ccgctgctgt gggcggatcg ccgcaaaggg tcttggctca acaaccgtga
     2341 agtcttcttg agaacgtatc gcaatacgtg catcctccac gaggtcatcg ccgggacgga
     2401 gatgctcaat cacgagttcc tcaccgatag tcgcgacgtg cagcggacga ccttttcgga
     2461 cggaactgtg gccatcgtca atttcgggag ccaaccgtat gagctccatg tgggcgataa
     2521 gagctatctc ctaccccaga atggctttta cgtgaaaggc ccccgaatcg aacagatccg
     2581 tgaacttgtt aacggtcgtg tcgttacgac catcaactct caagactatc agtaccggga
     2641 agaagaatag cggctccaag atgttttgcc gtaagcggta ggaaaacacc ctcgcgtccc
     2701 gggatatcac gactcctggt ttgccggact attgcggcgc tcgatgtcaa ctggacatcc
     2761 aagcctgtct gagcgcgtgt cggccttgta gcgtaattaa cccattcgga gcacggccag
     2821 attgaaggtt gctcgggtga gccgggttgc cttgcggtgg ccggctgtca gtgagaggat
     2881 ttcacccgga gatattgatt tcggggagtt cccccaccgt aggaacgacc atcggtggta
     2941 cgtcctgatc accccagcga gttgtcggtg ccggaggcaa ctggggcggt gcgggcgagg
     3001 taggcaattc ggccggtgcc ttctcaggtg gtggagccgg tgcggctggc ggtgacacgt
     3061 tttgcccatc cgtcggagcc attggaagtg ttccacctga ggaggagggt gccgtcggtt
     3121 gcggagccgg tgc
//
LOCUS       DAOPTR010000418         3131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_24777_length_3131_cov_3.456112, whole genome shotgun sequence.
ACCESSION   DAOPTR010000418 DAOPTR010000000
VERSION     DAOPTR010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24777_length_3131_cov_3.456112"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1898
                     /gene="ftsH"
                     /locus_tag="PKI05_08720"
     CDS             <1..1898
                     /gene="ftsH"
                     /locus_tag="PKI05_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011120515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOA52328.1"
                     /translation="LVEQGPPEKNPQAAVEVTRKIGNEIYRIKYSNPHDLVVRPHEVV
                     GKVTKEILSPDSKKTKAEDVTFRSSRLGFENDNNALLNLLLDRGFKGVRGGEPPTFWE
                     QYGPHIVLLLIFVLVMLFLLRRVGGPGSAIAFGRSRGRLYAQEDIGVTFNDVAGVDEA
                     VEEVREVVEFLKNPQKFRALGGRIPKGVLLVGPPGTGKTLLAKAIAGEAGVPFFSLSG
                     SDFVEMFVGVGAARVRDMFQQAQARAPCIVFIDELDALGKMRGTSIVGGHEEREQTLN
                     ALLVEMDGFNSNSGVIVLAATNRPETLDPALLRPGRFDRHILVDRPDVRGREAILKVH
                     CRNVKLDPSVNISEIAALTPGFVGADLANLVNEAALLAARKNKPTVTMEEFTEAVERV
                     TAGLEKRQRVMSEEEKRRVAYHESAHALVAYCLPNTDPVHKVSIIPRGIAGLGYTLQR
                     PEGDRYLLTQSELESRIQVLLAGNVAEELVFQDISTGAQNDLQRATDIARSMVTEFGM
                     SRLGRLCLRDDTPSHLGIYNDWTRPRWYSEATAEEIDREIRRILDEALERTRRILETR
                     RQALEALAQRLMEKEVIDKDELREIVEANSPGPVVVPGTEEVKPRREAPASAPRDAST
                     ASPEATP"
     gene            complement(2191..3126)
                     /locus_tag="PKI05_08725"
     CDS             complement(2191..3126)
                     /locus_tag="PKI05_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOA52329.1"
                     /translation="MGGLAILVDVLIPRKPLEIITSVYFGLIVGLFLSYVAGLALTPV
                     FESLQVDEQVRNVVQLIVSAILCYTCISFLFQTKDDFRFIIPYVEFTKEVRGRRPYIL
                     DTSVIIDGRIADLVETNLFDSRLIMPRFVLTELQAIADSADRTKRARGRRGLDILNRL
                     RNNPDVDFVIWDRDLPEFSGQSVDLKLITLAKHLEGRLVTNDYNLNKVARVHGVRVIN
                     INDLANALKPRFLPGEQIQVRLVRPGEEEQQGVGYLDDGTMVVVEDGREYVGRTVVLN
                     VTSVLQTSAGRMVFGRYDRTVEHNHEAREDRVARS"
BASE COUNT          677 a          913 c          908 g          633 t
ORIGIN      
        1 gtctggttga acaaggccca ccggagaaaa acccccaggc tgccgttgag gtgacacgga
       61 aaatcggcaa cgaaatttac cggattaagt attctaatcc ccacgatcta gtagttcgcc
      121 cccacgaggt tgttgggaaa gtcactaagg aaatcctttc ccccgactcg aaaaagacca
      181 aagccgagga cgtcacattc cgctcctccc ggctcggttt tgaaaatgat aacaatgccc
      241 ttttgaatct cctcctagac agagggttta aaggcgtccg cggtggcgag cctccgacct
      301 tttgggagca gtatggcccg catatcgtcc ttttgctgat ttttgtcctt gtcatgctgt
      361 ttttgctgcg acgggtgggc ggaccgggtt ctgccatcgc cttcggtcga agtcgcggac
      421 gtctctatgc ccaagaagat attggggtca ccttcaatga cgtggctggg gtagatgagg
      481 cggtcgaaga agtccgcgaa gttgtggagt ttctcaagaa tccccagaag ttccgcgccc
      541 taggtgggcg gattcccaag ggagtcctcc tcgtcgggcc accgggaacg ggcaagaccc
      601 tcttggccaa ggctatcgca ggcgaagcag gggtcccatt cttcagcctg tcgggttcag
      661 actttgtgga aatgttcgtc ggtgtcggag cagcgagggt ccgcgacatg ttccagcagg
      721 cccaggcccg ggcaccctgc atcgtcttca tcgacgagtt ggatgccctc ggcaagatgc
      781 ggggaacaag cattgtcggc gggcatgaag aacgcgaaca gaccctcaat gccttgctcg
      841 tggaaatgga cggcttcaac tccaacagtg gcgtgatcgt cctggcggcc accaaccgtc
      901 cggaaaccct cgaccccgcc ttgctccgtc ccggtcgctt cgatcgtcat atcttggtcg
      961 atcgccctga cgtgcgaggc cgcgaggcca tcctcaaggt tcactgtcgc aatgtgaagc
     1021 tcgacccctc ggtgaacatt agcgagattg cggccctcac gcccggattt gtcggggctg
     1081 atctggccaa cctggtcaac gaggcggctc ttctcgctgc tcggaagaat aaacccaccg
     1141 tgacgatgga agagtttacc gaggccgtgg agcgggtcac cgccggtctc gaaaaacgtc
     1201 agcgggtgat gagcgaggag gagaaacggc gggtggccta ccatgaaagc gcccacgcct
     1261 tggtggctta ttgcctgcct aacactgatc cggtccacaa ggtttccata atcccgcggg
     1321 gcatcgccgg ccttgggtac acactccagc gcccggaggg tgatcgttac ctcctcaccc
     1381 aaagcgaatt ggaaagccgc attcaggtcc ttttggccgg caacgtggcc gaggagcttg
     1441 tttttcagga catttccacg ggggcgcaga atgatctcca gcgggccacc gacatcgccc
     1501 gcagtatggt cacggagttt ggcatgagtc ggttagggcg gctatgtctt cgtgacgaca
     1561 cgccttcgca cttgggtatt tacaacgatt ggacgcgacc ccgctggtac agcgaggcca
     1621 ccgccgagga aatcgaccgc gagattcggc gcattcttga tgaggccctg gagaggacgc
     1681 ggcggatttt ggaaacccgt cgtcaggcct tggaggccct ggcccaacgc ctgatggaaa
     1741 aggaagtcat tgacaaagac gaactacggg aaattgtcga ggccaattct cccggccctg
     1801 tcgtcgtgcc gggtacggaa gaagttaaac cgcgccgcga agccccagct tcggcaccgc
     1861 gagacgcaag caccgcctca cccgaggcaa cgccctgaaa ttaagagcct atcccaaaaa
     1921 ctccccctct cctcttggga gagggttggg gtgagggcag ggagggacgc ggtcccccgc
     1981 gtccgtcatt tagcgcctat cccaaagttc cctccctccg ggagaggatt gcggtgaggg
     2041 caagcgtatg gcggaatcga tcgagagaat ccgcagcaat tcgagccacc atcacccccg
     2101 cccgctctcc cgccgaacgg gcgaggggag attatgggat aggctcttac cgaacacgat
     2161 ggcgctgccg cgcggttatc atatcttcga tcacgagcgg gcaactcggt cctcgcgggc
     2221 ttcgtggttg tgttcgacag tgcggtcgta gcgtccgaaa accatccgcc cagcactggt
     2281 ttggaggacg ctcgtcacat tgaggacgac cgtgcggccc acgtattctc gaccatcctc
     2341 cacaacgacc atcgtgccgt cgtcaagata acccacaccc tgttgctctt cctcccctgg
     2401 gcgaaccaaa cggacttgaa tttgttcacc cggcagaaac cgcggtttga gggcgttggc
     2461 taggtcgttg atattgatca cccgaacgcc gtggactcgg gccaccttat tgaggttgta
     2521 atcgtttgtc acaaggcgac cttccaggtg ctttgccagg gtgattagtt tcaggtccac
     2581 cgactgccca ctaaactctg gcagatcacg gtcccaaatg acaaaatcga catccggatt
     2641 gttacggaga cgattgagga tatccaggcc acgtcggccc ctcgcccgct tggtacggtc
     2701 ggcgctgtcg gcaatcgcct gcaattctgt taggacgaac cgcggcatga tgagtcggct
     2761 atcgaaaagg ttggtttcca ccaggtcagc gattcggcca tcaataatca cgctcgtgtc
     2821 cagaatgtaa ggccgccgcc ctctcacctc cttggtaaac tccacgtagg ggatgataaa
     2881 gcgaaaatcg tccttggtct gaaacaaaaa gctgatgcac gtgtaacaca agatggccga
     2941 gacaatcaac tggacgacat tgcggacttg ctcgtccact tgtaggctct caaagacggg
     3001 cgtcaaggcc aagcccgcta cgtagctgag gaacaagccc acgatcaagc caaaatagac
     3061 cgaggtgatg atctctaggg gcttccgggg aatcagaacg tccacaagga tggcaagtcc
     3121 gccgatggcc a
//
LOCUS       DAOPTR010000419         3129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_24794_length_3129_cov_3.574821, whole genome shotgun sequence.
ACCESSION   DAOPTR010000419 DAOPTR010000000
VERSION     DAOPTR010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24794_length_3129_cov_3.574821"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PKI05_08730"
     CDS             <1..427
                     /locus_tag="PKI05_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOA52330.1"
                     /translation="GVMLAKGDVATSLRPGMHASTFGGNPIAAAAGLATIEMIEEENL
                     LARAQHISRIFQERLVAMQQGCSIIREVRIVGLMIGIELTLEGAPVVQECLNRGLLIN
                     CTHNTVIRLLPAMVMTDEQVHEGCDILEDVLKKYAAGKL"
     gene            488..1423
                     /gene="argF"
                     /locus_tag="PKI05_08735"
     CDS             488..1423
                     /gene="argF"
                     /locus_tag="PKI05_08735"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOA52331.1"
                     /translation="MMKHRTCRHILTVEDLSTAEIERIFSISEDLKAKYAEGIREPLL
                     PHRVMALIFEKPSLRTRVSFEAAMTHLGGNAIFLGDDAGFGKRESIADFARVLSQYVD
                     VIVFRGKAHQTVEELAKYSECPVINGLTDYAHPCQALADLFTIRELAGKLEGHTLAWV
                     GDGNNVARSLAEGCAKLGMRFVMATPEGYRFEADFLPRLRAKYPKFEFEVFDRPEDAV
                     RDALVVYTDVWTSMGQEAEAEKRRRDFHAYQVNGRLMAHTQKGAVFMHCLPAHRGEEV
                     TDEVIDGPQSVVIQQAANRMHVQKGILAWLLASHS"
     gene            1514..2263
                     /gene="rph"
                     /locus_tag="PKI05_08740"
     CDS             1514..2263
                     /gene="rph"
                     /locus_tag="PKI05_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOA52332.1"
                     /translation="MDQSGVLVRADGRAWNELRPITIERRFTSAAPGSVLITAGRTRV
                     LCTASVDPQVPPWLVGQGTGWITAEYSMLPGSTSPRKQRERSRVEGRSAEIQRLIGRS
                     LRAVTDLSALGERTITVDCDVLQADGGTRTLSITGGFIALVDALRMILPELSDPSRLP
                     LRDSVAAVSVALWGENLILDPDQREDNEAAVDCNIVMTGSGKFVEVQGTAERVPFDRF
                     LLERMLDLAANGITQLTRLQREALGEDWPWT"
     gene            2312..3082
                     /locus_tag="PKI05_08745"
     CDS             2312..3082
                     /locus_tag="PKI05_08745"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOA52333.1"
                     /translation="MMKSSLWLSAGDGVPAKLALWGLVIVSIGLACRPMPPVGNDSQN
                     ASRQRQRREFHEVKYTDKSPEEWLTLLGHRNSQVRDQAIDALLQYGPEQIKPLIKIAA
                     DKSNPRARLSAIRALGAFGPKAAEASAVLVEALEDQSWDGRDAAAEALGLIKQATPEV
                     LAALVRALHDPDERVRMASARALGRCRTSDPAAIEGLTQALGESDMNVQLAAIEALAE
                     IGPAAKASLGELQRIAETGPTLVRASAQEAIKRINSSP"
BASE COUNT          663 a          767 c         1013 g          686 t
ORIGIN      
        1 tggtgtgatg ttggcgaaag gggacgtcgc caccagtttg cgaccgggaa tgcacgcctc
       61 cacgtttggt ggcaatccga tcgcggccgc cgcgggactg gcgaccatcg agatgatcga
      121 ggaggagaac ctccttgctc gtgcacagca tattagccga atctttcagg aacgtttggt
      181 ggccatgcag caaggctgct cgatcatccg ggaagttcgg attgtgggac ttatgatcgg
      241 gatcgagcta acattggagg gcgcacctgt cgtgcaggag tgtctcaacc ggggcctact
      301 catcaactgc acgcacaata cggtgatccg attgttgccg gccatggtga tgacggatga
      361 gcaggtccac gagggatgcg atattttgga ggatgtgctg aaaaaatacg ccgcgggaaa
      421 gttgtgattc atcattctgc ggcaggcagt tttcatagat taggataaac gggaatctga
      481 gggcggtatg atgaagcatc ggacttgtcg ccacattctg actgtggaag atctttcaac
      541 cgctgagatt gaacgaatct tttcgatcag tgaggacctc aaggctaaat acgctgaggg
      601 tattcgggag ccgttgttac cgcatcgcgt aatggccctg atttttgaga agccgtcctt
      661 gcggacgcga gtcagtttcg aggccgccat gactcacctg ggggggaatg ccattttctt
      721 gggcgatgat gcgggttttg gcaagcgaga gtcgatcgcc gattttgccc gtgtcttgag
      781 ccaatacgtg gatgtgatcg tttttcgcgg taaggcccat cagacggttg aggaattggc
      841 caaatattcg gaatgcccgg tcatcaatgg cctaactgac tatgctcatc cgtgtcaggc
      901 gttggcagat ttgttcacaa ttcgcgaact ggcgggcaaa ctggaagggc acaccctggc
      961 atgggttggc gatggcaata acgttgcgcg aagcctggcc gagggctgcg ccaaattggg
     1021 catgcggttt gtgatggcga cacccgaagg ttaccgcttt gaggcggact ttctgccgcg
     1081 attgcgggcc aagtatccga agtttgaatt tgaggtgttc gatcgcccgg aagacgctgt
     1141 ccgcgatgcc ttagtagttt atacggatgt atggaccagt atgggacaag aagccgaagc
     1201 cgagaagcga cgtcgtgatt ttcacgccta tcaggtcaat gggcgtctca tggctcacac
     1261 ccaaaaaggt gccgtcttca tgcactgttt gccggctcac cgaggggaag aggttaccga
     1321 cgaggtgatt gatgggccac aaagcgtggt gattcaacag gcggctaacc gaatgcatgt
     1381 gcaaaagggt atcttggcgt ggctattggc ttcgcattcc tgagatgcga aggctttttt
     1441 acaaacggac tttggcgagc agttcgctcg tcacgctgtg ccgggtggtc agtacggaga
     1501 ggggatgtga gcgatggacc aatcgggtgt gttggttcga gcagacggtc gggcgtggaa
     1561 tgagctgcgg cccattacga ttgagcgacg atttacgtct gccgcaccgg ggagtgtcct
     1621 tatcacggca gggcgaacgc gggtcctgtg tacggccagc gtggatcccc aggttccccc
     1681 atggctagtg gggcagggga cgggctggat caccgctgaa tacagcatgt tgcccggcag
     1741 tacctctccg cgaaagcaac gagaacgatc ccgtgtggaa ggccgttcgg ccgagattca
     1801 gcgactgatc gggcgaagtc tgcgggccgt gacggacttg agcgcccttg gtgagcgaac
     1861 gatcaccgtg gattgtgacg tccttcaggc cgacgggggc acgcggacgt tgagcatcac
     1921 gggcgggttc atcgccttag tggatgccct gcggatgatc ctgccggaac ttagcgatcc
     1981 gtcgcgattg ccccttcgag atagtgttgc tgccgtcagt gtggccctct ggggggaaaa
     2041 ccttatcctc gatccggacc agcgggagga taatgaggcg gcagtcgatt gcaacatcgt
     2101 gatgacaggt agcggaaaat ttgtggaggt ccagggtacg gccgagcgcg ttccctttga
     2161 tcgtttcctg ctcgaacgaa tgctcgattt ggccgcgaac gggatcactc agttgacacg
     2221 tcttcaacga gaggcgctgg gggaggattg gccctggacc tagcaagcga ttccaatcgg
     2281 tgaaggactt tctgccgagt tgaaagagct tatgatgaaa tcatctttgt ggttatctgc
     2341 gggcgacggg gttccggcga agttggccct ttggggactg gttatcgtaa gtatcggtct
     2401 ggcatgtcgg cccatgcctc ccgtgggaaa cgactctcaa aatgcttcac gccagagaca
     2461 gcggagagaa ttccacgagg tcaagtacac ggacaagagc ccggaggaat ggctgacgct
     2521 tttagggcac cgtaactcgc aggtccgcga tcaggccatt gatgcccttt tgcaatatgg
     2581 gcccgaacaa atcaaaccgc tgataaagat cgcagccgac aaatcgaatc caagggcccg
     2641 gctttctgcg atcagggcct tgggggcatt tggaccaaag gccgctgagg cgtcggcggt
     2701 tcttgttgaa gcattggaag atcagtcttg ggatgggcgg gatgcagcgg cggaagccct
     2761 aggactgatc aagcaggcaa ccccggaagt cctggccgca ttggtccgtg cgcttcacga
     2821 tcccgatgag cgggttcgga tggcttcggc tagggcgtta ggacggtgcc gaacaagtga
     2881 tcccgcggca atcgagggac tgacgcaggc cctcggtgag tcagatatga acgtgcaact
     2941 ggcggctatc gaggcgctgg cggaaattgg cccggctgcc aaagcgtcgc ttggcgaatt
     3001 gcaaaggatt gcagaaacag gccccacgct ggttcgggct tcggcccaag aggccatcaa
     3061 gcgcatcaat tcgtcccctt aaagcctatt ccatagtttc cccttatgtg ttctgaggga
     3121 gaggggtcg
//
LOCUS       DAOPTR010000420         3123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_24861_length_3123_cov_3.772490, whole genome shotgun sequence.
ACCESSION   DAOPTR010000420 DAOPTR010000000
VERSION     DAOPTR010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24861_length_3123_cov_3.772490"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PKI05_08750"
     CDS             <1..834
                     /locus_tag="PKI05_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52334.1"
                     /translation="IDVDGELPRQLVMKKQWYSFLLWGRLSYDPQLSNDHFRKILAAR
                     FPEVPSDQLFAAWVEASQVFPYITRFFWGDIDLRWFPEACTRHPAPKGFYTVKDFILG
                     ETMPGSGVLSIREWLSQGEKAGETAGVTPLQIAETLETHSREALRLLKELKPKEKPRR
                     ELQRTLGDLEAMAYLGQYYSAKIRGAVALAEFDRDGKPEQQEAAVKHLEIALAHWKHY
                     ADIAHSQYKPQLLNRIGFVNLHEIAGYVEKDIAIAKQWQPGTLKDIPPGPRTADVPFR
                     P"
     gene            complement(1762..2781)
                     /locus_tag="PKI05_08755"
     CDS             complement(1762..2781)
                     /locus_tag="PKI05_08755"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOA52335.1"
                     /translation="MPADHADRLSRRDFVCQASAFAGAAVLGSVVSSEQARSVESPSV
                     GPLPRRTLGRTGESITIFTLGTAPCGNARPVDEIAEVVNAAIDLGVTSIDTAPAYVRA
                     EEGVGKVMKTRRKEVFLATKVPADSVEEGQRSLEKSLRLLQTDYVDLLYYHSVGNRKV
                     EGALDPDGAFTWLVKQKQAGKCRFIGISGHNLPGRFAQFLETGEVDVLLVVLSYVDRH
                     TYNFEEKVLPIARKHNVGIIAMKVLGGADPKKGGYGNPQSVGRLASARPDRVETAIRY
                     ALSLPGVTSVNLGINSVEQLKQDLVYFQRFEPLSEAEMDQLLEEGRELAAAWGAHFGP
                     VVEEA"
BASE COUNT          661 a          895 c          857 g          710 t
ORIGIN      
        1 attgacgtcg atggtgagct tccgcgacaa ctcgtgatga agaagcagtg gtacagcttt
       61 ttgctttggg ggcgactgag ctacgatccc cagctttcta acgaccattt ccgtaagatt
      121 ttggctgcca gatttccgga ggtgccttcc gatcagttgt ttgccgcttg ggtcgaagcg
      181 tcgcaggtgt tcccctatat cacgcgattc ttctgggggg atattgacct gcggtggttt
      241 cccgaggcct gcacacggca ccccgcccca aagggctttt acacggtcaa ggactttatc
      301 cttggggaga ccatgccggg gagcggtgtg ctgagtattc gcgagtggct ctctcagggc
      361 gaaaaggctg gagagaccgc aggggtcaca cccttgcaga ttgccgaaac tctggaaacg
      421 cattcacgag aagcgttgag attgttaaag gaattgaaac caaaggaaaa gccccggcgc
      481 gaacttcaac ggacattggg cgatttggag gccatggcgt atctgggcca gtactattcc
      541 gcaaagatta ggggagcggt ggccttggcg gagttcgacc gtgatggcaa acccgaacag
      601 caagaagccg ctgtaaagca tctcgaaata gccctggcgc actggaagca ctatgcggat
      661 attgcccaca gtcagtacaa accccaattg ctcaaccgga tcggatttgt aaatcttcac
      721 gagattgcgg ggtatgttga aaaggacatc gccattgcca aacaatggca gcctgggacg
      781 cttaaggaca tccctcccgg accccgtaca gccgatgttc ctttccgccc ctaggtgacc
      841 cagtagagcc gatccaggca gattttttgg cggaccctgt ccgctttctc ttaaggttcc
      901 cgaggcgaaa cgtgccgggg cgtgctacgc cctcgggcgt tttctttttg ccacacagcg
      961 ttgttgggtt gttgctcagg ctccgcacac gtcgaacttg tttgattggc atcagctcgc
     1021 acaagttcat aattaaacga cacttacgcc tattctgatg aggcccagat gtcgcggcgc
     1081 ggtgattgcg acactccgtc acgcggggga gttttttcga aggatcgcgt ccgtcggttc
     1141 ggctgctgac ccgttgatca aggccgcgtg cgaccatggc ttttcgtatt cgcccaagtt
     1201 tcctctgggt cattctgctt gcattgactg ccctcggggc tttcgccgcg gtcgtaccaa
     1261 catcctctcc aagggcgacg ggctacaatg agccagactc tctttggcgt aagacgaagg
     1321 acggctggga aatcgcctgg tggcttcagc cgccgaaaac tttccacaaa ccggtgatcc
     1381 atcccctcgc cgtggcggca tgtcaattac tggtcgtgtc gttactggga ctgatcaacc
     1441 accatcagac cagccgcgat cggcaatcct cgcgcgtcct ccaaggtcaa tccgctggag
     1501 tgaggagaat agacaaaacc gcagcaccca cgactttccc gtcgtttgtg tcccgtggtg
     1561 atgcgcccaa gcgagactct gagagccttt cacaccaacg gggtttggat cgcatttaac
     1621 gccgcggatc tcttcttcgc gagctccgcc acgtggaccg ctttgtgagg gggcaagggt
     1681 atggtggaca tccttccgcg gccgcgcggc actcctcgcc cgtactactg gcactcgccg
     1741 gcgttggagt cgtgatcagc cctaggcctc ctcaaccact gggccgaaat gggctcccca
     1801 agcggcggcc aattcgcgtc cttcttccaa tagttggtcc atctcggcct cgctcagcgg
     1861 ctcaaagcgt tggaaataga ccaaatcttg tttcagttgt tctacgctat tgatccccaa
     1921 attaacgctt gtcacccctg gcagcgaaag ggcgtaccgg atcgctgttt caacgcggtc
     1981 cggcctggcc gacgcaagtc gtcccacgga ctgtgggtta ccgtagccgc cctttttggg
     2041 gtccgcaccg ccgaggacct tcatagcgat gatccccacg ttatgtttgc gggctatagg
     2101 gagaaccttc tcctcgaagt tgtaggtgtg gcgatcaaca taactgagaa cgaccagcag
     2161 aacatccacc tctcccgtct cgagaaactg cgcaaatcgg cccggcagat tatgcccgga
     2221 gatcccaata aaccgacatt tcccagcctg cttctgtttt accaaccacg tgaatgcccc
     2281 gtccggatcg agggcccctt ccaccttgcg attaccaaca ctgtgatagt aaagcaaatc
     2341 tacgtagtcg gtttgcagga ggcgaaggga tttttccagg gagcgctgac cttcctccac
     2401 agagtcggcc gggactttcg tggccagaaa aacttccttg cgacgcgtct tcataacctt
     2461 tccgacacct tcttccgcgc gaacgtacgc gggcgcagtg tcgatcgaag tcacgccaag
     2521 gtcaatggcc gcgttgacga cctcggcaat ttcgtccacg ggtcgggcat ttccacacgg
     2581 ggcggtgcca agggtgaaga tggtgatgga ctctccggta cgtcctagcg ttcgccgagg
     2641 aagtggtccc acggaaggag attccacaga ccgggcctgc tccgacgata ctacactgcc
     2701 taatacggcg gcaccggcga acgcactcgc ttggcaaacg aaatcacgac gcgaaagacg
     2761 atcagcatga tcagcgggca tggctaactc ctgatacttt gtgttgtcaa tgcaagtaac
     2821 gaccttccct cctaatcgcc acacaaccgt aaaatatccg gggcttgggg accctcgtac
     2881 cattacggct atgtggccag gcatttaagt cacccataat tgtttcgttt tctaacccaa
     2941 agtcaaaaga gtgacaacag ggagccggag cgagtgatcg tcttacatat tctgcctctt
     3001 caatgatggc cagcaggcgc gctcatcgcg taattcagtt tgcatgagtt gccaatgaat
     3061 tgcatcggct cttaattgat ggcataatcg gggcgggttc cctggtgttt tgggctgaca
     3121 gtc
//
LOCUS       DAOPTR010000421         3119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_24904_length_3119_cov_3.244778, whole genome shotgun sequence.
ACCESSION   DAOPTR010000421 DAOPTR010000000
VERSION     DAOPTR010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24904_length_3119_cov_3.244778"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            375..2672
                     /locus_tag="PKI05_08760"
     CDS             375..2672
                     /locus_tag="PKI05_08760"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOA52336.1"
                     /translation="MMSELIRVSHPTALSPAVTAAMMTPTPIQRETLHGPPKPIDVFH
                     AVRRRWWLILLLGTIGAVAVMIFAWRTIPVQYTATAWLQVSLQRPRIIFDTREDDPLL
                     RNRQTQATLVTSNRVLGAALRQPGISQLPYIRNQIDPMMWLRQKIRVRFADQAEILEI
                     SMTGEDPEQIAAIVNAVKDAYMAEVGDVNRTYALERKRLLEQNYAQTLELVKKRDLRY
                     RELANAVGSADSEVARRKAITALETLADHRRRVNEIRQQLGGLDRKITLLQTRLKYLE
                     EHRPQSSEEERRERDEAVIRQIVEAGLRADPWISSAQARLSALEARWFEERQRVVQWE
                     KSPTILRLQEEMQALREAIENRRNELIPGLTQYAREQLEKGRAKAAPTEAELIQAQIA
                     EAELERAVLEDELKVALEQFQKEAAEAEQLGKYSADLEAEKAEVDRLKAVAAKMGDEL
                     ERWNVELAAPPRVRVLEEASTPRVSNINDKYRLVTFLGLMTFLGTAAVVVLLDFLLRP
                     VSSAYQISYGLGLPVLGDLPLIQRGPFSRITSNGQMPEHVKTILLESVDQLRTVLLHR
                     AKRDGVKSVLVTSALAREGKTTLASQLAVSMARAGRRVILVDGDLRRPRLHRVLRRDL
                     HPGLAELARNEATPQEIVQTADLEGLYLVTAGHCDAAALEAVSQGRLDGFLNSLRDTF
                     DMVLIDSSPLLTTADSMILAHLADGVVLSVVQDRTRLPRLFEAVQRLRAVDLPLIGIV
                     IHGTAPSRYRSYYRSYTIDVEVRKS"
     gene            2769..>3119
                     /locus_tag="PKI05_08765"
     CDS             2769..>3119
                     /locus_tag="PKI05_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA52337.1"
                     /translation="MKNSWIVVLGLTLFLSRTVFGQAQPQPLNVVFFLVDDLGWTDVG
                     CFGSTFYETPNIDRLAAEGMRFLDAYAACQVCSPTRAAIQTGKWPQRAAITDYIGAPQ
                     PADWNRYTRLLPAAY"
BASE COUNT          671 a          811 c          972 g          665 t
ORIGIN      
        1 atcccaaaaa gtccccctct cccttcggga gagggttggg gtgagggcaa gcgaatgtcg
       61 gaatcgattg agaaaaccgg cagcgattcg aggcaccctc acccccgacc cctctcccgc
      121 cgaacgcgtt aggggagatt atgggatagg ctttttagct ggcgcgtctt tgccctgcgc
      181 gtggaatttc gaggggtttt tagtcaaaat ggggagaggt atacggttga taaggacggt
      241 aatggacgta tgggcagggg gttgaagatt gccgtgagat taggccgggg gcactgcgtc
      301 gtagaacggg gtaactgtgt gcacgttaaa cattcctgcg ctttgaaaag agacgacgat
      361 tgccctgaga tggaatgatg tccgagttga tacgggtttc tcacccaaca gcactttcgc
      421 ccgccgtaac ggcggcgatg atgacgccaa cgcctatcca acgggagacg ttgcatgggc
      481 caccgaagcc gattgacgtt ttccacgcgg tacgacggcg gtggtggctg attcttttgc
      541 tggggacgat tggagctgtg gccgtcatga ttttcgcctg gcgcaccatt cccgtccagt
      601 acacagccac ggcatggttg caagtctcct tgcagcgtcc gcggattatt tttgacacgc
      661 gcgaggatga tcccctcctt aggaatcgtc aaacgcaagc caccttagtc acgagcaacc
      721 gagtcttggg ggctgccctg cgtcagccgg ggattagcca acttccgtat atccgcaacc
      781 aaattgatcc catgatgtgg ctacggcaaa agatccgcgt gcgatttgcc gatcaggcgg
      841 agatcctgga gatcagtatg acgggagagg accccgagca gatcgcagcc atcgtcaacg
      901 cggtcaagga cgcctacatg gccgaggtgg gggacgtcaa ccgaacatac gccctggagc
      961 gcaaacgcct gttggagcaa aactacgccc aaacgttgga gttggtcaaa aagcgggacc
     1021 tccgttatcg tgaattggcc aacgcagtgg ggagtgccga ttctgaagtc gcccggcgga
     1081 aggcgatcac ggcgctggaa acgctggcgg accatcggcg gcgcgtgaat gagattcgac
     1141 agcaactggg aggtttggat cgcaagatca cgctcttaca gacacggtta aagtacctgg
     1201 aagagcaccg tccgcaaagc tcggaagaag aacgccgtga gcgcgatgag gcggtcattc
     1261 ggcagattgt ggaggccggc ctccgcgcgg atccttggat ttcctcggcc caggcgaggc
     1321 tatcggcatt ggaggcccgc tggtttgagg aacgacagag ggtcgttcaa tgggagaagt
     1381 caccgacgat tctccgcctc caagaggaga tgcaagccct ccgcgaagca attgagaacc
     1441 gtcgcaacga attaatcccg gggttaaccc aatacgcgcg ggagcagtta gagaaagggc
     1501 gggcaaaagc cgcccccacg gaggccgaac ttatccaggc ccaaattgcg gaagctgaat
     1561 tagaacgggc tgtgctcgag gatgaactaa aagtggccct ggaacagttt cagaaggagg
     1621 ctgccgaggc cgaacaactt ggcaaatact cggccgatct ggaggccgaa aaagcggagg
     1681 tggatcgtct caaggccgtg gcggcaaaaa tgggagatga gttggagcga tggaacgtgg
     1741 agttggcggc gccgcctcgg gttcgtgtgt tggaagaggc aagcacgcct cgggtcagca
     1801 atatcaatga caagtaccgc ctggtaacct tcctggggtt gatgacattt ttgggaacag
     1861 ctgccgtcgt agttttgcta gattttcttc tccgccctgt tagctccgcc tatcaaatca
     1921 gctacggtct cggtttgcct gtgctgggcg atctgccact cattcagcgc ggtcctttct
     1981 cccgcatcac ttcaaatggc caaatgccgg agcatgttaa gacgatcttg ttggagtccg
     2041 ttgatcagct tcgcacggtg ctcctccatc gggcaaaacg ggatggtgtg aagtcggtct
     2101 tggtgacaag tgccttggct cgggagggca agacgaccct tgccagtcag ttggcggtta
     2161 gcatggctcg ggcggggcga cgcgttatct tggtggatgg tgacctacgg cggccacgtt
     2221 tgcatcgtgt gcttcggcga gatctgcatc ccggactggc ggaattggcc cgaaatgagg
     2281 cgacgcccca agagattgtc caaacggctg accttgaggg gctctacttg gtgacggctg
     2341 ggcattgcga tgccgctgcc ctggaggcgg tctcccaggg aaggctcgat ggctttctca
     2401 attccctccg cgacacattt gatatggttt tgatcgattc cagtccgctc ctgacaaccg
     2461 cggattccat gattctggcc cacctggcag atggggttgt cctttccgtg gtccaagacc
     2521 gaactcgctt gccacggctc ttcgaggccg tccaacgatt gcgggccgtc gatctgcccc
     2581 taatcgggat tgtaatccat ggcacggcgc ccagtcggta ccgcagttat taccggtcgt
     2641 acacaattga tgtagaggtt cgaaagtcgt gagccgactt tcagctcatg ggtcaaacaa
     2701 tgaacgggat gtccgggacg gtatcgcgga gtttcttgga agagttaggc tggttgggga
     2761 ggttcatcat gaagaacagt tggattgttg ttctgggact gacgctcttt ttgagcagga
     2821 cagttttcgg ccaggcgcag ccccaacctt tgaacgtcgt gtttttcctg gtagatgacc
     2881 taggttggac ggatgtgggc tgtttcggga gcacatttta cgaaacgccc aacattgatc
     2941 ggctggcggc ggaagggatg cgatttttgg acgcttacgc ggcctgccag gtatgttccc
     3001 cgacaagagc cgctattcag accggtaaat ggccacaacg ggcggccatt accgattaca
     3061 taggggcccc ccaaccagcg gactggaacc gatacaccag gcttcttccg gcagcctat
//
LOCUS       DAOPTR010000422         3115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_24941_length_3115_cov_4.159477, whole genome shotgun sequence.
ACCESSION   DAOPTR010000422 DAOPTR010000000
VERSION     DAOPTR010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24941_length_3115_cov_4.159477"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="PKI05_08770"
     CDS             <1..676
                     /locus_tag="PKI05_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52338.1"
                     /translation="GKFDPNRDPGDFAPQSPIRQEVDRLAFFVGPVHVRFEGDEKNSR
                     MLDLSKFIDRKKGIVRSMTGEISLDFRNGVCTVNTPKCQGCTGFLKQGGSRFSFDDMT
                     IVSENDYATIMVVTMDNRPLRDSEKVLVQVGTTARMTGWTERDAEFESGGQKLRGKQI
                     VDTGRPPWRLAENRIKMTVKNSGLGRAVVLDVDGYPRQDLPLQRQGDGLTLEMPRDCL
                     YVILMK"
     gene            764..2284
                     /locus_tag="PKI05_08775"
     CDS             764..2284
                     /locus_tag="PKI05_08775"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOA52339.1"
                     /translation="MKRHNERAANHEALRHSRRGFLAITAQLAAFGFVGCGRVTACAE
                     DSVSLFDGRSMEGWRASENKDSWRVEGGCLVADGPRSHLFYEGPVCDHNFRNFDLTLE
                     VLTKPGANSGVFIHTQYQETGSPSAGYEVQINNSYPMVGDNPEFRRTGGLFAVQDVFK
                     AFLPDDQWFQMRILVIGKRIRVWVNEFLMVDYVEPESPPRMKNRVGRRLSRGTIALQA
                     HDPNSRIHFRNIRIRPLPDQADPFADPRPDVSPYGITEGLIDRLSMSAIPATDFHIHL
                     RGGMTVEKAIERQAVTGINAGVLENHGRGWPLDNNEKLAAFLESVAGRPVFVGIQVND
                     RDWAEVIDPKLLDQLDYVLADTMIMPMPDDNSPPVKLWQTDQYTIDDPEAWMERYMKH
                     NLRVLSEPITILANPTWLPKPIEHLYDKLWTEERMRLLIETAVKRGIALEINAGKYPP
                     EKFIRLARELGAKFTLGTNNFDDRPISLARCFDMIDRVQLQGRNFLLLRPKDKQVG"
     gene            2506..3093
                     /locus_tag="PKI05_08780"
     CDS             2506..3093
                     /locus_tag="PKI05_08780"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOA52340.1"
                     /translation="MVKRAPIARICVFFFITVMGVAADLTTKAWIFNRLGMPGEKPVW
                     WVIPDVFGFQTSLNEGALFGMGQGMTRWFAALSVVFAVGIVVWFFRGGAGHSWWMTTS
                     LAAITAGIFGNLYDRLGLHGLRWHEADGLHQVGDPVYAVRDWILVMIGRWPWPNFNIA
                     DSLLVCGVTALVVYTLFFSELACPSEEKARVEETK"
BASE COUNT          673 a          805 c          911 g          726 t
ORIGIN      
        1 tggcaaattc gatcccaacc gcgaccccgg ggatttcgcg ccccagtcac cgatcagaca
       61 ggaagtcgat cgcctggcat tctttgtcgg accagtccat gtcagattcg aaggcgatga
      121 aaaaaatagc cgaatgttgg atctatccaa gtttattgat cgcaaaaagg gaatcgtgcg
      181 gagtatgacg ggcgaaatct cgctcgattt tcgcaacggc gtgtgcaccg ttaacactcc
      241 caagtgccag ggttgcacgg gtttcctgaa acagggaggt agtcgcttct cgtttgatga
      301 catgacaatc gtgtcggaaa acgattacgc cacgatcatg gtggtgacga tggacaaccg
      361 tcccttgcgt gattccgaaa aggtccttgt ccaagtaggt actacggcac gaatgacggg
      421 ctggacggag cgcgacgcag agtttgaatc gggcggccaa aagctccgcg gcaaacagat
      481 cgtcgatacc ggtcggccac cgtggcgatt ggcagaaaac cgaattaaga tgaccgtcaa
      541 gaattccgga ttagggcggg ctgtggtgct cgatgtggac ggttatcccc gtcaggacct
      601 tcctttgcaa cggcaaggag atggtctgac tctggaaatg ccacgggatt gcttgtatgt
      661 catcttgatg aagtgagaaa gattgtttgt cgttcatata ggccgggcac agctcggata
      721 gatattttcg tcttacccca ccagcatata aaggaaccat cctgtgaaac ggcataacga
      781 acgagctgca aatcacgagg cgttgcggca ttcacgacgg gggtttctgg cgatcacggc
      841 ccaactggct gccttcggat tcgtcggctg tggcagagtc acggcctgcg ccgaagattc
      901 ggtcagcttg ttcgatggtc gctcgatgga aggatggcgg gcatcggaaa acaaagacag
      961 ttggcgggtg gaaggaggct gtcttgtggc cgacggccca cggagccacc tgttttacga
     1021 aggccccgtg tgcgaccaca acttccgcaa tttcgatctc acgcttgaag tcctgaccaa
     1081 gcccggggcc aattccggcg tttttattca cacgcagtat caagagaccg gttccccaag
     1141 cgccggttat gaggttcaga tcaataattc ttatcccatg gtgggcgaca atccggaatt
     1201 tcgccgcacg gggggactgt ttgcggtcca agacgttttt aaggcctttt tgccggatga
     1261 tcagtggttc cagatgcgga tcctggtaat cggcaagcgg attcgtgtct gggtcaatga
     1321 gttcttgatg gtggactatg tcgaaccaga atccccgcct cggatgaaaa atcgtgtggg
     1381 gcgacggctt tcccgcggca cgattgccct gcaagcccac gatcccaata gtcgcattca
     1441 ctttcgtaac attcgtatcc gccccttgcc ggatcaagca gacccctttg cggacccccg
     1501 accggatgtc tcaccctacg gcataacgga gggcctcatt gaccgcctgt ccatgtcggc
     1561 cattccggct accgatttcc atatccacct ccggggcggg atgaccgtgg agaaggccat
     1621 cgagcgacag gccgtcaccg gcatcaatgc tggggtgctg gaaaaccacg ggcgcggttg
     1681 gcccctggac aataatgaaa aacttgcggc tttcttggag agtgtggcag gacgccccgt
     1741 attcgtgggg atccaggtca acgaccgtga ttgggcggag gtgatcgatc ctaaacttct
     1801 cgatcagctc gattacgtgc ttgccgatac catgatcatg cccatgccgg atgacaacag
     1861 tccaccggtc aaattgtggc agacggatca atataccatc gacgatcccg aggcgtggat
     1921 ggaacggtac atgaagcaca atctgcgggt cctctcggag ccgattacca tcctcgccaa
     1981 tccgacctgg ctgcccaaac ccatcgaaca tctttatgac aagctttgga ccgaggaacg
     2041 gatgcggcta ctcattgaga cggctgtcaa acggggtatc gccctcgaga tcaatgcggg
     2101 caagtatcct cccgagaagt ttatccgcct ggcgcgggag ctaggggcca aatttacctt
     2161 gggaaccaat aacttcgacg atcgtccgat ctcacttgcc cgctgctttg acatgatcga
     2221 ccgtgtgcag ttacagggtc gaaacttcct tttgttgcgg ccgaaggaca aacaagtggg
     2281 ataaccgacg cgggcctttg gtggttcccg gcctggcagc gtctgcgcgg gcacttttgg
     2341 gaagcgctgg tgcgattgtc ccaatgttgc ttagggaatg ttccattttg ccaggaaatc
     2401 ccctttacat tttcttggtg cttttttaga accatcaaga gatcgttgtg ttgaggtcca
     2461 gccgcgagga taaggatttg gggaagcgtt agaaaggaat gatcagtggt caagcgagca
     2521 ccaatcgccc gcatctgcgt ttttttcttt atcacggtga tgggcgtggc ggcagacctt
     2581 acgacaaagg cgtggatctt taatcgtctg ggaatgcccg gcgagaaacc cgtctggtgg
     2641 gtgattcccg atgttttcgg ctttcaaaca agccttaatg agggggcgct ctttgggatg
     2701 ggtcaaggca tgacgcgttg gtttgccgcc ctctctgtgg tgttcgccgt ggggattgtc
     2761 gtgtggtttt tccggggggg tgccggccac agttggtgga tgacgacgag ccttgcggcc
     2821 attaccgccg ggattttcgg aaacctctac gaccgtcttg gtcttcatgg cctaagatgg
     2881 catgaggccg atgggctcca ccaggtgggt gaccccgttt atgccgtccg ggattggatc
     2941 ctagtaatga tcggtcgttg gccctggccg aacttcaaca ttgccgacag ccttttggtc
     3001 tgcggcgtca cggcgttggt ggtatatact ttgtttttct cggagctagc atgccccagc
     3061 gaggaaaaag cacgggtcga ggaaacaaag tagtcactct tgtaagtacg tttcc
//
LOCUS       DAOPTR010000423         3113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_24963_length_3113_cov_4.115762, whole genome shotgun sequence.
ACCESSION   DAOPTR010000423 DAOPTR010000000
VERSION     DAOPTR010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24963_length_3113_cov_4.115762"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="PKI05_08785"
     CDS             <1..1287
                     /locus_tag="PKI05_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009095261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HOA52341.1"
                     /translation="PYFAIPPKTFWQGWAQSSALVGCLMGAAISGALSDRLGRRRLLI
                     TAGFLFTLSAIWTAIAWDYTSFNLARITGGIGIGLASNLSPMYIAEIAPAEIRGVFVS
                     INQLTIVIGILAAQITNLLIAQPVPQDIPADQITAFIRESWNGQFGWRWMFGAETFPA
                     VGFFLLMFFVPESPRWLVKSGYDTLAERILARVGGPDYARHAVADIRQTLSAEAIARV
                     RLGDLLERRLLRVVALGVFLAVFQQWCGINVIFNYAQEVFQAAGYAVSDVLVNIVFTG
                     IVNLVFTFVAIFTVDRWGRRFLMLFGAGGLALIFGLLGLGYYFHSTGWHMLVLVLAAI
                     GCYAMSLAPITWVVISEIFPNRIRGLAMSVAVLSLWAACTALTLTFPILNRNLGPHGT
                     FWLYAGVCAVGFLTILATLPETKGKTLEEIERELVR"
     gene            complement(1297..2325)
                     /locus_tag="PKI05_08790"
     CDS             complement(1297..2325)
                     /locus_tag="PKI05_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOA52342.1"
                     /translation="MTSITLTRVTKWSSFQHGWVTGILVCLLIASSALGRDIFVNNES
                     GDDSFTGGIAEAGQRVDGPVRTIARALALAQPGDRIVLAKTNTPYRESLTLAGKRLSK
                     DNLGDFILEGNGAILDGSAPVPPEAWQVDKDSWQRFKRAVFRFTPEGKSYQQLFLGDR
                     PAVRVYADRLSAVPPQLEPMQWALFQGSIYFCVERDRHPADYPLRYAKLPVGITLHHV
                     EGVTIRDLVIQGFQMDGINLANTARDVRLERVVLRGNGRTGLTVGGACRGTLIDSLVG
                     DNGFAQILTLPNSEIHVQKCQVLGNTAPAWVDEGGRFFLAGELRKGGLDDIDAEAGTP
                     QTSDPGQN"
     gene            2597..>3113
                     /locus_tag="PKI05_08795"
     CDS             2597..>3113
                     /locus_tag="PKI05_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HOA52343.1"
                     /translation="MNELHEECGVAAVYHLPGRPVSPLCPGGPMSAPRLIPRLLLDIQ
                     NRGQLAAGITRYCEGDSQLLLTYKNVGTVAEVFSLAQRTRFDDLMTRFSGSAALGHVR
                     YATCGKDDPSYAQPFERHHIKKHKWFSFAFNGQLANYLELRERLLRDEDNHLIRDTDT
                     EVIMHVLCRELN"
BASE COUNT          630 a          910 c          860 g          713 t
ORIGIN      
        1 ccttattttg ccatcccacc gaagaccttt tggcaaggtt gggcccagag cagtgctttg
       61 gtcggatgcc tcatgggagc ggccatttcc ggggccctca gtgaccgctt ggggcggcgg
      121 cgtctcttga ttacggccgg attcctcttc accctttcgg caatctggac ggccatcgct
      181 tgggactata cctcgtttaa tcttgctcga atcaccggtg ggattgggat cggcctggcc
      241 tccaatcttt cgcccatgta catcgcggag attgccccag ccgagattcg cggcgtgttc
      301 gtgtccatca accagttgac aatcgttatt ggtattctcg cggcccagat cacgaattta
      361 ctcattgccc agcctgtacc ccaggatatc ccggcagatc agatcactgc gttcatccgc
      421 gagtcgtgga acgggcagtt tggatggcgc tggatgtttg gagccgaaac atttccggct
      481 gtgggattct tcctcctcat gttctttgtc ccggaaagcc ctcgctggct ggtgaaatcc
      541 ggctacgaca ccttggccga aagaatcctc gcccgagtgg gcggccccga ttacgcccgc
      601 cacgccgtgg ccgacattcg ccagacgctc agtgcggaag ccatagctcg ggtgcggctc
      661 ggcgaccttt tggagcggcg tttgcttcgg gttgtggctc ttggggtctt tttggctgtg
      721 tttcagcagt ggtgcggcat caatgtgatc ttcaattatg cccaagaggt cttccaggct
      781 gccggttacg ccgtcagtga cgtccttgtg aacattgttt tcactgggat tgtcaacctg
      841 gtgtttacgt ttgtggccat tttcacagtg gatcgttggg gccgacgctt tctcatgctc
      901 tttggggccg ggggcctggc tcttatcttt ggcctccttg ggctgggtta ctatttccac
      961 agtacgggct ggcacatgct ggtgctggtc cttgcggcga tcggctgcta cgccatgtcc
     1021 ttggccccga ttacgtgggt tgtcatctcg gaaatcttcc ccaatcggat tcgtggactg
     1081 gccatgtcgg tggctgtcct gtccttgtgg gcagcctgca cagccctgac gttaacgttc
     1141 cctatcctca accgcaatct gggtccccac gggacgtttt ggctttatgc gggcgtctgc
     1201 gcggttggtt ttctcacaat actggcgaca ttgccagaaa ccaagggtaa gaccttggag
     1261 gagatcgagc gcgaacttgt aagatgaggc caacgatcaa ttttggccgg gatccgaggt
     1321 ctgcggtgtg ccagcctccg cgtcgatgtc gtcaagacca cccttccgaa gttcgcccgc
     1381 aaggaaaaac cggccaccct catcgaccca ggccggggcc gtatttccca gaacctgaca
     1441 cttttggaca tggatttccg aattgggaag cgtcaggatt tgtgcaaagc cgttatcacc
     1501 aacaagtgaa tcgatgagtg tcccccggca cgcaccaccc accgtgagac ccgttcgccc
     1561 gttgccccgc aagaccaccc tttccagacg gacatcccgt gcggtattgg ccaaattgat
     1621 tccatccatt tgaaagccct ggataaccag gtcgcggatc gtcacccctt ccacatgatg
     1681 aagtgtgatc cccacaggca acttcgcata acgcagggga tagtcggcgg ggtgccgatc
     1741 tcgctccacg caaaaataga tgctcccttg gaagagggcc cactgcatgg gttccaattg
     1801 cggcggaacc gccgataaac gatcggcata gacgcgcacg gccggacgat caccgaggaa
     1861 aagctgctga tagctttttc cttcgggcgt gaatcgaaaa acagcccgtt tgaatcgctg
     1921 ccaggaatcc ttgtccactt gccaggcctc gggcggcacg ggagccgagc cgtccaagat
     1981 cgccccatta ccttccagga taaaatcccc aaggttgtcc ttgctcaaac gtttcccggc
     2041 cagagtcagc gactcccgat acggggtgtt ggttttcgca agcactattc gatcgcccgg
     2101 ttgtgccaag gcaagggcgc gggcaatcgt gcgaacaggt ccatccaccc tctgtcccgc
     2161 ctcggcaatc cccccggtaa aagaatcatc gcccgattcg ttgttgacaa aaatgtcacg
     2221 tcccaaggca gaggaggcaa tgagcaaaca aacgagaatt cccgtcaccc aaccgtgttg
     2281 gaaggaagac catttcgtaa cccgcgtcaa cgttatagac gtcatatctc tcgttccaat
     2341 atgggttaca cgtcttggaa atgtcgtcac caaattccaa cggattacag gaaagaatct
     2401 tttggtccag tgtatcatca ccaaaatcga atcgccaccg aatcgggagc ggccaggaca
     2461 gagcgtggcc cccacccggc tcgttgtagc gaatgccttc aaaccgtaaa ataagagagg
     2521 aagtgctacc tatccccggg gtcgggcggg ttgtctgccg acaatgcccg gaactctctt
     2581 tgaggcggga acatcaatga acgaacttca cgaggagtgc ggcgttgccg cagtctatca
     2641 tctgccgggt cgtccggtga gtcccctttg tcccggcggc ccaatgtcgg cgccgcgact
     2701 catcccgcgg ttgttgctcg acattcaaaa ccgaggccaa cttgctgcag gaatcactcg
     2761 ctactgtgag ggggactcgc agctcctttt gacctataag aacgtgggga cggtcgccga
     2821 ggtcttttcc ctcgcgcagc ggacgcggtt cgacgatcta atgacaaggt tctcggggtc
     2881 ggcagccctg ggacacgttc gctacgcaac ctgtgggaag gacgacccta gctacgcaca
     2941 accctttgaa cggcaccaca ttaagaagca caaatggttc agtttcgcct tcaacggaca
     3001 actggctaac tacctagagt tgcgcgaacg gctactgcgg gacgaggata accatctgat
     3061 ccgcgacacc gacacggaag ttattatgca cgtgctgtgt cgggagctta acc
//
LOCUS       DAOPTR010000424         3106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_25051_length_3106_cov_3.679122, whole genome shotgun sequence.
ACCESSION   DAOPTR010000424 DAOPTR010000000
VERSION     DAOPTR010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25051_length_3106_cov_3.679122"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PKI05_08800"
     CDS             complement(<1..382)
                     /locus_tag="PKI05_08800"
                     /inference="COORDINATES: protein motif:HMM:NF017346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HOA52344.1"
                     /translation="MMRFQGIAVSSGVAIGKAVVIGQDVFSIPSQYVAADAVEQEISR
                     FHQAVETAAKAIERNRDLVTQELGPKSGAIFQAHLEILRDQKIHADIERLIREKQYSP
                     ERACTEVLRRYADVFRRLPNPEFAE"
     gene            complement(441..797)
                     /locus_tag="PKI05_08805"
     CDS             complement(441..797)
                     /locus_tag="PKI05_08805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOA52345.1"
                     /translation="MSLTSGNSAVKRRWVTIRNRAGLHTRAATLVRETVLKHRSRVFL
                     IKPENNGECLDGAPRADATSVIEMLSLCALEGTPLVLEAEGEDADRVLDELQNLVNCK
                     FWEDDFAPESEEANHA"
     gene            complement(990..1469)
                     /locus_tag="PKI05_08810"
     CDS             complement(990..1469)
                     /locus_tag="PKI05_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOA52346.1"
                     /translation="MKFRDFVVKDAIVTDLDPYLTKEEIIRKLCESLVTAGALKREQL
                     DDIVKALIKREELGTTGIGMGVAVPHTKHPSVDRLVATIGISPEGVDFDALDGERVQS
                     LFLLVSPTDLPGDHLRALEYITRQLHNSTFARFLKQAKTIDEVWTLLEEADNGKYTH"
     gene            2121..3062
                     /locus_tag="PKI05_08815"
     CDS             2121..3062
                     /locus_tag="PKI05_08815"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOA52347.1"
                     /translation="MPDKAFLGHEQAFSKKREPGPFDPFFKAVWRGAATILPPLITFV
                     IIIWIVTTINQYIFSPLTRAVRDLIFVATADIVTSSERSPEIVRDGKTYVRFKGGKYV
                     PIEVVNYIREEIGEDKLPATATEVYQLFIEERYLKPHLVLPFLIAILVLLLYLVGKIV
                     TVRIGRITWAQIESLFSSLPFVRSVYGAVKQVSDFILAQRSMEFSRVVAVEWPRRGMW
                     MIGLVTSESFSQLQTKTGQEMLSVFIPTSPAPMTGFTVNVPISDTFDLGISIDQAIQF
                     IVSCGVVVPIPEAHGVTLDQQKHPTASPSNPADSDSE"
BASE COUNT          674 a          886 c          782 g          764 t
ORIGIN      
        1 gttcagcaaa ctccggattg gggagccgcc ggaagacatc tgcataccgc cgtaagacct
       61 cggtgcatgc cctctccggg gaatactgtt tttcgcggat cagtctctcg atatctgcat
      121 ggatcttttg gtcccgaagg atttccagat gggcctgaaa aatggccccc gatttcggcc
      181 ccaactcctg cgtgacaaga tcacggtttc gctcgatggc tttcgccgcc gtctcgaccg
      241 cctggtggaa acggcttatc tcttgctcaa ccgcgtccgc cgcgacgtat tggctcggga
      301 tggagaagac gtcttgtccg ataacaacgg ccttgccaat ggcgacccct gaagaaacag
      361 caataccctg aaaccgcatc attgcttgat ctcagacttc ccgtggggga caaatccccg
      421 atgccgcctt gacatgccgt ttacgcatga tttgcctctt ccgactctgg ggcaaagtcg
      481 tcctcccaaa acttgcaatt cacgagattc tgcaattcat cgagtaccct gtctgcgtct
      541 tctccttcgg cctccagaac aagaggggtc ccttccaggg cacagaggct tagcatctcg
      601 atcacgcttg tcgcgtcggc tcgcggtgcg ccgtccaaac actccccgtt attttccggt
      661 ttgattaaga aaacgcggct gcggtgcttg agaacggtct cacgcaccag ggtagctgcc
      721 cgtgtgtgca gccccgcccg gttacgaatc gtaacccaac gcctttttac ggctgaattt
      781 cccgaagtca aactcatgtc ttcctttgat tcgtgccaac aaggtcgttg tcggccacta
      841 cgaacttccg actcactggg aaaaatgtcg tgaaatcacg aggacatcaa accaacaagt
      901 ctcagcggtt tgttttctcg aggtcacacc ccggatggca caaactgttt ctcgggtccc
      961 gcagcggagc cggtgggagg cacgtccagt tagtgcgtgt acttgccgtt gtccgcttcc
     1021 tcaaggagtg tccacacctc gtcgatcgtt ttggcttgtt tcaagaatcg cgcaaacgtg
     1081 gagttgtgaa gctgtctcgt gatgtactcc aaggccctca ggtgatcacc tggaaggtcc
     1141 gttggtgaca ccaaaaggaa gagcgactgg acgcgctcgc catccagggc atcgaagtcc
     1201 accccctcgg ggctgatacc gatggttgcc acgagccggt ccacgctggg gtgcttcgta
     1261 tgggggacgg ccacccccat gccgattcca gtcgtgccaa gttcttcccg cttgatgagc
     1321 gcttttacaa tgtcgtcaag ctgttcgcgc ttcagcgctc cagccgtcac aagtgattcg
     1381 cacagcttgc gaataatctc ttccttggtg agatagggat ccaaatccgt gacaatcgcg
     1441 tccttcacca caaagtcgcg gaatttcatg cggtaaattc cctttcctga aaccaacggg
     1501 ccagcggaat cacgcaatgg atattccgcc ggctctcaca acacctggac atatcggccg
     1561 aagcatctcg ctaaggcctc ttgtcgaact tcagttatcc ttgctcgtgt tttctttcaa
     1621 accactcgaa atcgcccgat cacctgttcc cacatctgcc ggatggtgtg cttcaagttc
     1681 ggtcggcact atccatcagg gtgcccggta acatgccaag ccactcgggc ggcccacaag
     1741 ctcataggct catcgaacta ccggcctcaa aaatcacaca acaattctac cttttaagaa
     1801 acccgccgga ggccgaaact ctcagtttat cccaggcatc attcgacgcc aatggtatgc
     1861 cgctccacgg atgcagtgcc ataccccacc gatgtggggt gccgtccaca taaaggacgt
     1921 gagcctatca atcaccgata ggccttcgtg ggcctggagc agatcacaca ctgacggtcg
     1981 tactctcgtt taccccggtt cggcattctt tagtacaatg tatctcgtta tcataaggga
     2041 taagcatcat tagttaaacg ttcgcgctgt gcgggcaatc tgtaccgacg gaaccatccc
     2101 aacatttttt cggagtttat atgccggata aagcattcct cggacatgaa caggcgtttt
     2161 cgaaaaaacg ggaaccaggg ccgttcgatc ctttttttaa ggcagtttgg cgcggagctg
     2221 ccacaatatt gccaccgttg atcacatttg tgatcattat ttggatcgtc acaacgatca
     2281 atcaatacat tttttcgccc cttacaaggg ctgttcgcga cctaattttc gtcgccacgg
     2341 ccgacattgt gacctcttcc gagagaagcc ccgagattgt ccgcgacgga aagacatatg
     2401 tccgcttcaa gggggggaag tatgtaccga tagaggtggt caactatatt cgggaagaga
     2461 taggcgagga caagctcccg gcaaccgcca cggaggtcta tcaattattc attgaggagc
     2521 gatatcttaa accgcatctt gtcctgccat ttttgatcgc aatcttggta ttgctgcttt
     2581 acctggttgg caagattgtc acggtacgaa tcgggcggat cacctgggcg caaatcgagt
     2641 cgcttttttc ttcacttcct tttgttcgga gcgtctatgg agctgtcaag caagttagtg
     2701 attttattct tgcacaacga tccatggaat ttagccgggt cgtggccgtg gaatggccac
     2761 gccgcggaat gtggatgatc ggccttgtca cgagcgagag cttctcccag cttcagacga
     2821 agaccggcca agagatgcta agtgtcttca ttccgacttc tcctgctccg atgacgggtt
     2881 ttaccgtcaa tgtgccgatc agtgatactt tcgatctggg catctcgatc gatcaggcga
     2941 ttcaattcat cgtgagttgt ggggttgttg ttcccatccc cgaggctcac ggtgtcacac
     3001 ttgatcaaca aaagcacccg accgcgtctc cgtcgaatcc cgcggatagc gattccgagt
     3061 aggacgttcc acatgaggtg cgagcatccg tggccgtgac tcggtg
//
LOCUS       DAOPTR010000425         3098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_25120_length_3098_cov_3.028262, whole genome shotgun sequence.
ACCESSION   DAOPTR010000425 DAOPTR010000000
VERSION     DAOPTR010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25120_length_3098_cov_3.028262"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PKI05_08820"
     CDS             complement(<1..298)
                     /locus_tag="PKI05_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52348.1"
                     /translation="MALEAPIVDETNPAPVDDPMQDPFGDSTQVKSAPLPQHLAPSSP
                     PQMLSQGNRPEPLEVLPTPPPLISQAAPTQRGPVTTEVLSQGPGEASKSTETRQT"
     gene            complement(675..2165)
                     /gene="guaB"
                     /locus_tag="PKI05_08825"
     CDS             complement(675..2165)
                     /gene="guaB"
                     /locus_tag="PKI05_08825"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOA52349.1"
                     /translation="MFAHEKIIGHAITFDDVLLVPGYSEVVPAEVDVSTQLTPRIRLR
                     IPILSSPMDTVTESAMAIALAQEGGIGIIHKNMSIARQTAEVEKVKRTANGIIDNPAT
                     LPPTASAAEARELMDQLNVSGIPIVLPDRKLVGIVTRRDLRFLGNLNVPVSEVMTKEN
                     LVTATGTITLSEAEKILMGKKVEKLLLVDEDFRLTGLITIKDIDNQRRFPNACKDEYG
                     RLRVGAAVGVHDYQRAESLIKTGVDVLCVDSAHGHSANVLETVRELKRQFSGHNVDII
                     AGNVATYEGARAVIEAGADAVKVGIGPGSICTTRVVTGVGVPQITAIMAAVEAAKATG
                     TPVIADGGIRYSGDITKAIAAGAHAVMLGGLLAGLDESPGQRILYRGRVYKAYRGMGS
                     LGAMVHGSSERYRQGDAHQPEKLVPEGVEGRVPYRGPLGPFVYQLVGGLKAGMGYCGA
                     RNIEELRTKARFIQVSVAGVRESHPHDITITQEAPNYTVDYAGEEH"
     gene            complement(2420..>3098)
                     /locus_tag="PKI05_08830"
     CDS             complement(2420..>3098)
                     /locus_tag="PKI05_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52350.1"
                     /translation="VTVRVEMLVEVAGKRFRPEPQTVRQGFHGELLTSDSKIKDLGST
                     EVIVQGRSITCQVLEVETVNAGVRTLSKITYSPTVAPYVIQRDSIRTELAGNRILESS
                     HMEVTILDVPCRVGLRVLSAAQTRTVQTFPRGSVVTLSLVSGDVPGGVVCQTTKETDD
                     TRKLVRRTTLELVDFGLRPGLRYSGGLRWRLLPREVYKLPQEWIQPGLLPETQGITWP
                     AIQANEG"
BASE COUNT          650 a          890 c          855 g          703 t
ORIGIN      
        1 gggtctgccg ggtttcagtt gatttcgatg cctcgcccgg accctgtgaa aggacctccg
       61 tcgtgactgg tccacgctgc gttggcgccg cttgagatat caaaggcggc ggggtgggca
      121 atacctccag tggttcaggt cgattgccct ggcttagcat ctgcggtggc gaagatggcg
      181 ccaggtgctg gggaagtggg gcagacttca cctgcgtcga gtccccaaac gggtcctgca
      241 tggggtcatc gacgggtgct gggtttgttt catcaacaat gggggcctct aatgccactg
      301 caagactctc tgtctccgag tcagcggatg tggagtttgt gatcgccggt agttggggtg
      361 ttggtgaaag ctgcatcacg ggagaaagag gcgttgtcac ggagttgacc ggagacggtt
      421 gcggtggaag ccactgtaac ccgtcggaac ttgcagtcga tgccacggct tcgtgcgaag
      481 taagaacaac ttgcttagaa gacgatggga aagttgctat ggcttgtcgt ctttctccgg
      541 ttggcttccg tcgggctgtc cattcagagg ccccaatctc gaccaagggg cacaagcact
      601 gaaaaacgat gatcaaacca acacaccgag aaactacgcg gaagaagggt gacttccgcg
      661 aaaaggggtt tgaatcagtg ttcctcgccg gcgtaatcaa cggtgtagtt gggcgcttcc
      721 tgcgtgatcg tgatgtcatg gggatgactc tccctaaccc cagcaaccga gacctgaatg
      781 aaccgggctt ttgtgcgaag ctcctcgata tttcgggcac cgcaataacc catccctgct
      841 ttcaggccac caaccaactg ataaacaaac ggtccaaggg gaccccgata aggcacccgg
      901 ccttcgacgc cttcgggaac caatttctct ggctggtggg catctccctg ccgatatcgt
      961 tcgcttgatc catgaaccat ggctcccagg gaacccattc cccgatatgc cttgtacacc
     1021 ctcccgcgat ataggatcct ctggcctgga ctttcatcga gccctgccaa gagccctccg
     1081 agcatcactg catgcgcccc ggcggcaatc gctttcgtga tatcccctga ataccgaatt
     1141 cctccgtcag cgatcactgg ggttcccgtt gccttggcag cctcaacagc cgccataatc
     1201 gcagtgattt gagggactcc cacgcccgtt accactcgtg tggtgcaaat tgatccagga
     1261 ccaatcccca ccttgacggc atcggcccca gcctcgatca ccgcccgggc gccttcgtag
     1321 gtggcaacgt tgcccgcgat tatatccacg ttatggccgc taaattgacg cttgagctca
     1381 cggactgtct cgagcacgtt ggccgaatga ccgtgagcac tatcgacaca taggacgtca
     1441 actcccgtct taattaaact ctctgctcgc tggtagtcat ggacacccac ggccgccccc
     1501 acgcggagcc gcccatattc atccttgcaa gcgttgggga aacgccgctg gttgtcgatg
     1561 tccttgatcg ttataagtcc cgtcagtcta aaatcttcgt caaccagcag aagtttctcc
     1621 acctttttcc ccattaggat tttttcagct tcgctgagcg ttatagttcc cgtggccgtt
     1681 acaaggttct ccttcgtcat aacttccgag accggcacat tgaggttgcc gagaaacctg
     1741 aggtctcggc gggtgacaat cccgacaagc ttccggtcgg gaagcacaat cggaataccc
     1801 gagacattga gttggtccat caactctcga gcctctgccg ccgaggcggt aggcgggagc
     1861 gtggccgggt tatcaatgat cccgttcgcc gttcgtttga ccttctccac ctctgccgtt
     1921 tgacgcgcaa ttgacatgtt cttgtggatg atgccgatcc ccccttcctg ggcaagcgca
     1981 atcgccatag cgctttcagt cacggtgtcc atgggcgagc tcaagatggg gatgcgaaga
     2041 cggatcctcg gcgtcagttg cgtgctgacg tcaacttctg ccggaaccac ttcactgtag
     2101 ccagggacga gcaagacatc gtcaaaagtg attgcatgac cgatgatctt ttcatgggca
     2161 aacatgggtg gcctccattg acccgtgcgt gtcgtgtgtg ttttctgggg aatgttttag
     2221 tattgaacaa gcaaatcgag atgacaagga cgtcggggtg aaacagagcg taggacagac
     2281 aacacgtagt aggcaagcgg cgtgtgaatc tccactccgg ccaataggag agatgctggg
     2341 aaacaccctc cgctggcgca aactaccgtc cgtggtttgc ttggcccccg gtgaagcggg
     2401 tcattggcaa tcggtggggc tatccttcat tggcctgtat ggcaggccag gtgatacctt
     2461 gggtttctgg cagtaacccg ggttggatcc actcctgggg aagcttgtaa acttcgcgcg
     2521 gcaataaacg ccaacgtagg ccgccgctgt atctgagccc tgggcgaaga ccaaaatcca
     2581 ccagctccag tgtcgtgcgc cgcacgagct ttcttgtgtc gtctgtctcc ttagtcgtct
     2641 gacataccac accgccgggc acatccccgg acactaaaga gagcgtgacg accgagccgc
     2701 gcggaaatgt ttggactgtc cgagtctgag ctgctgaaag gacccgtagt ccgacccgac
     2761 agggaacatc caagatcgtc acctccatat ggcttgattc gaggatccga tttcctgcca
     2821 actcggtgcg aatcgaatcc cgttggatta cgtaaggagc aaccgtcggt gaatacgtaa
     2881 tcttgctgag agtccgaacg cctgcgttga cggtttcgac ttccaagact tggcatgtaa
     2941 tcgatcgacc ttgaacgatc acctccgtcg atccaaggtc cttgattttt gaatcggagg
     3001 tcagcagttc cccatgaaag ccctgccgga cagtctgagg ctctggccga aatcttttcc
     3061 ccgccacctc cacaagcatt tccacgcgaa cggtgacc
//
LOCUS       DAOPTR010000426         3082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_25276_length_3082_cov_3.423852, whole genome shotgun sequence.
ACCESSION   DAOPTR010000426 DAOPTR010000000
VERSION     DAOPTR010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25276_length_3082_cov_3.423852"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1086..2657
                     /locus_tag="PKI05_08835"
     CDS             1086..2657
                     /locus_tag="PKI05_08835"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOA52351.1"
                     /translation="MNEANPKTQTRRIVIFDTTLRDGEQSPGCSMNLQEKLEVAHALA
                     QLGVDVIEAGFPIASAGAFEAVKPIAESVRGPVICGLARCRPEDIDRAWEALRFAESP
                     RIHVFLATSAIHREHKLRMTKEQIIDVAVAGVRRAVGYCPNVEFSPEDAVRTEIDFLC
                     QVVEAAIDAGATTINIPDTVGYATPGQMFRLITTLRERVPNIGKAVISVHCHNDLGMA
                     VANSLAAVEAGAGQVECTINGIGERAGNCALEEVVMALRVRQDIYHADTGIVSQRLVP
                     TSRLVAGITGMVVQRNKAIVGQNAFAHEAGIHQDGILKEPKTYEIMRPEDVGFSRSDL
                     VLGKHSGRAALAARAKALGYQLTPEQLDRVFREFKILADKKKEVYDGDLAALIEQETR
                     VVTDTWSLESYQLTVGTQRVPEVTMTLRRADNELRTLTLAAGDGPVDAVFLAIEKITG
                     ITVACRDFRVHSVTVGKDALGEVQVQVEYNGEIFRGRGVSTDSLEASAKAFLNAINRV
                     LAKNHRPKVVSEETS"
     gene            2940..3024
                     /locus_tag="PKI05_08840"
     tRNA            2940..3024
                     /locus_tag="PKI05_08840"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2973..2975,aa:Leu,seq:taa)
BASE COUNT          763 a          815 c          887 g          617 t
ORIGIN      
        1 cccaaaaagt ccccctctcc ctccgggaga gggttggggt gagggcaagc gaacgccagg
       61 ctcgattgag aaaaccggca gcgattcaag tcaccctaac ccccgacccc tctcccgccg
      121 aatgggagag gggagattat gggataggcc cttcgcccct cccataaagt ctacccactt
      181 ttgagacccc aactcgccgg gaaacagggg gttggacgag aattgttcgg gccgcagcaa
      241 ttgtggcgaa ttaataacgg aggtccgttt cggagaatcg accctgcaga aatggggcct
      301 cacagatgca agtcgcgtca aaaccacaaa ataggcaagg cgtgcatttt accccaggaa
      361 gcgaacatgt gaaggcgacc gcgctcgcaa gctcaaacca ggactgtgca ccatagccgc
      421 aaaaaagtaa ctaaaagttg gtaaactccg agccaatccc gccttgacga gatgaaacct
      481 aggggataaa gttattgaaa gttgtggaag acatgaggga aacaatgcac ctgatcaatt
      541 gcgtgaacct gctgttgggc ttgttgttat tgcctcgcca atgaggctgg ccctacggtt
      601 cgcgcagtag attaggtaaa aatggactat cgaacccagg gccaagcaaa ccctgggttt
      661 tttgtttgac actaagagtc tatcccaaaa agtccccctc tccctgtggg agagggttgg
      721 ggtgagggca agggactgtc ggagtccagc aagaaaaccg gcagtgattc gagccaccct
      781 cacccccgac ccctctcccg cccaacgggt gaggggagat tatgagataa gctttaagcg
      841 gcaagatggc ctccagcgta attgaccgct aagactcgca gaaggtgacc gtcaggcctg
      901 caaacgtttt aggcagtacg gaaaaattat ggacctcgtg atccgcaaaa gcaccataat
      961 acagatggaa aaacggcgat tggctcccac tcgggcatgg ttgttattgc gacgacccta
     1021 cctaccgcgt ttgtgaaggt gttactttga gttcggagtc tcttggcgaa tcagggtcga
     1081 agaccatgaa cgaagcaaat ccaaaaacgc aaacacgcag aattgttatc ttcgatacga
     1141 cgctccgcga cggggaacaa tcaccgggct gcagcatgaa cctccaagaa aagttggagg
     1201 tggctcacgc cttagcacaa ctgggcgtgg atgttatcga ggccggtttc ccgattgcct
     1261 ccgccggcgc tttcgaggcc gtcaagccga ttgccgagtc agtgcgtggt cctgtcattt
     1321 gcggccttgc ccggtgccgt ccggaagaca tcgaccgcgc ttgggaagcc ctccgctttg
     1381 ccgaatcccc gcgcattcat gtcttcctcg cgacgagcgc catccaccgg gaacataagc
     1441 ttcgcatgac gaaagaacag atcatcgacg tggccgtggc tggggtgcga cgagccgttg
     1501 gatactgccc caacgttgag ttctcgccgg aggatgcagt ccgcacggag atcgatttcc
     1561 tctgccaggt ggtcgaggcc gccattgacg ccggtgccac cacgattaat atccccgaca
     1621 ccgtagggta cgccaccccc ggccaaatgt tccggttgat cacaacactt cgggaacgag
     1681 ttcctaacat cggaaaggcg gtcatcagcg ttcattgcca taacgacctg ggcatggctg
     1741 ttgccaatag tctagctgcc gtggaggcgg gagcgggaca ggtcgagtgt acgatcaatg
     1801 gcatcggcga gcgggcagga aattgcgcac tcgaagaagt tgtcatggcc ctccgggtcc
     1861 gccaagacat ttaccatgcc gacaccggga tcgtgagcca acggcttgtc ccaaccagcc
     1921 gactggtggc aggaattacc ggcatggtgg tccaacggaa taaagccatc gtcgggcaaa
     1981 acgcctttgc ccacgaggcc ggtattcatc aagacgggat cctcaaggag ccaaagactt
     2041 acgagatcat gcggcctgag gatgtgggct tttcgcgcag cgacctggtg ctcggcaagc
     2101 acagcggacg tgccgcactc gctgctcgcg ccaaggccct cggttatcaa ttgaccccgg
     2161 aacagttgga tcgcgtgttt cgtgaattca aaatccttgc cgacaagaaa aaagaggttt
     2221 acgatggtga cctggcggcc ctcatcgagc aggaaacgcg ggtcgttacg gacacatggt
     2281 ctttagaatc ctaccaactg acggtaggca cccagcgtgt tcccgaggtc acgatgacac
     2341 tccgccgggc cgacaacgag ctgagaaccc tcacactggc ggccggcgac ggacccgtcg
     2401 atgcggtgtt cttggctatc gagaagatca cgggcatcac agtcgcctgt cgggattttc
     2461 gcgtccacag tgtaactgtc ggcaaagacg ccctgggtga ggttcaggtc caggtcgagt
     2521 ataatgggga aatcttccga ggacgcggtg tgtccacgga tagcctggag gcaagtgcga
     2581 aagcattttt gaatgctatt aaccgcgtac ttgcgaagaa ccaccgacca aaggtcgtct
     2641 cggaagagac ttcataaaag agagaaaagt atcataattt gggtattgca tttgccacga
     2701 tcaaccaggg acttcctaac acatagggaa gtacctgacg gagcgtgtat tactgtacgg
     2761 aagaacagtt ccttttagag taaggtctct gacaggcagg gaaccgcctg aggagcgcgg
     2821 aaggattata gcggagagat aatttacgta ccatgaagcg ttatatgggt accacgaact
     2881 ttggtgcctt taggtcgtaa gtactgacca agttcctgag atatggtgtt cattgaggtg
     2941 gccgagtggc ggaatggcag acgcacagga cttaaaatcc tgtggggagt tatccccgtg
     3001 cgggttcgaa tcccgcctcg gctattgggg tatttgcact agtcggcaac atagcgcaaa
     3061 acgcggaaaa aacggggttt tc
//
LOCUS       DAOPTR010000427         3077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_25325_length_3077_cov_2.914626, whole genome shotgun sequence.
ACCESSION   DAOPTR010000427 DAOPTR010000000
VERSION     DAOPTR010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25325_length_3077_cov_2.914626"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PKI05_08845"
     CDS             complement(<1..499)
                     /locus_tag="PKI05_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HOA52352.1"
                     /translation="MTPGGSPRQTLTFLRERFAAAGIRPKTDLGQNFLIDYNLLRVLV
                     ETAEVSSQDVVLEVGTGTGSLTNMLAERAAYVVSVEIDPRLHALARQLVGEKPNVCLL
                     CQDALKNKNAIHPGIIERIRQALDVDPQRRLKLVANLPYCVATPVISNLLGLPNPPVS
                     MTATIQ"
     gene            complement(813..1415)
                     /locus_tag="PKI05_08850"
     CDS             complement(813..1415)
                     /locus_tag="PKI05_08850"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOA52353.1"
                     /translation="MFSGIVEALGTVVDVISEPPGVRLIVKEPRIAAETRVADSIAVN
                     GCCLTVIDVQGETFAFQAGPETLSRTNLGDLKPGSRVNLERSLKVGDRLGGHFVTGHI
                     DDVGFLAERQDFGEWSRFWFRIPRRLAKQMAPKGSIAVDGVSLTIVDSEPDRFSVALI
                     PYTLAVTTLGPMKVGDKVNLETDILAKYVERQLEAQHWVS"
     gene            1764..2516
                     /locus_tag="PKI05_08855"
     CDS             1764..2516
                     /locus_tag="PKI05_08855"
                     /inference="COORDINATES: protein motif:HMM:NF024878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOA52354.1"
                     /translation="MAVGKSGCPVKTILTVLEKCLATQARSPVPDAWQVEIATMLAPI
                     GCVAMPDEIVKKAFRGEPLKENEVATYQTHPRIAHDLISKIPRLEQVARIVAYQEKLY
                     NGGGFPADLVEGDDIPLGARIIKLAFDWDSLISGGLSPELALAEITDRRGWYDPKVVN
                     ALRRILNVHQVCVVREMKISDLVDRLVLADNVYSVNGTLLCSKGQEVTPAIRFRLRNY
                     AVNVGIARPIRSFVPTVGAVNELSALSEPNSE"
     gene            complement(2662..>3077)
                     /locus_tag="PKI05_08860"
     CDS             complement(2662..>3077)
                     /locus_tag="PKI05_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylgalactosamine-6-sulfatase"
                     /protein_id="HOA52355.1"
                     /translation="KSSVFSAMDLVPSLLEIAGIAAPSDVRFDGENLASALLGKSSDS
                     RKAPLFWRRPPDRKFFAGKGPYPDLAVRVGDWKLLCEYDGSKPELYNLANDPGETQNV
                     ADKHPDVVRRLTELVLGWNQSMPQDNGPTLGQSGP"
BASE COUNT          682 a          932 c          815 g          648 t
ORIGIN      
        1 gctggatcgt cgccgtcatc gacacgggag gattagggag gccaagcaga ttgctgatta
       61 ccggcgttgc cacacaatag ggaagattgg ccaccaactt caatcggcgc tgaggatcca
      121 catcgagggc ctgccggatc cgctcaatga tcccgggatg aatcgcgttt ttgttcttca
      181 aagcgtcctg gcacaaaaga cagacattcg gcttctctcc gaccaactgg cgggctaagg
      241 cgtgcagcct gggatcgatt tcgaccgaca cgacgtaagc cgctcgttcg gccagcatgt
      301 tggtcagact gccagtcccc gtcccaacct ccagcaccac atcctgcgag gacacttcgg
      361 ccgtctcaac tagaacccgg agcagattat agtcgatcaa gaaattttga ccgaggtcgg
      421 tcttcggccg aatgccagcc gccgcaaacc gctccctgag aaatgtcagc gtttgtcggg
      481 gagaaccacc tggtgtcatt ccgctactgc gtccttcacc acccatacgg cttttgcact
      541 cgccagcgat tatcgctttg caacttgcca gtaaacggca gggaatcgct tgaagacctc
      601 attcgtggct ccgacccatt ctggaaaaac ccaccacgcc cgcctttttc gttagagcct
      661 atcccataat tcccctgaca tggtcgtcgg gagaggggtc ggggctgacg gtgcctcgaa
      721 tcgctgccgg ttttctcaac cgattccgac gatcgcttgc cctcacccca accctttccc
      781 aaagggagag ggggaatttt tgggatagac tcttagctta cccaatgctg ggcctccagt
      841 tgacgctcca catatttggc caggatgtcg gtctcgagat tgaccttgtc gccgactttc
      901 atggggccaa gcgtcgtaac ggccaaggtg tacggaataa gggccacact aaagcggtcc
      961 ggttcgctat cgacaatcgt cagactaaca ccatccacag caatggagcc cttgggggcc
     1021 atctgttttg caagacgccg agggatgcgg aaccagaacc gcgaccactc gccgaagtcc
     1081 tgtcgctccg ccaaaaaacc gacgtcgtca atgtggcccg tcacaaaatg tccacctaac
     1141 cgatccccta ccttaaggga ccgctccaaa ttgactctac ttccgggttt aagatcgcca
     1201 agattcgttc gagaaagcgt ttcaggaccc gcttgaaagg caaaggtctc cccctgcacg
     1261 tcgataactg tcaggcaaca gccattgacc gcaatgctat cggccacacg ggtctccgca
     1321 gcgatcctcg gctctttcac aatgagtcgc acacccgggg gctccgaaat cacatcaaca
     1381 actgtcccca aggcctcgac gatcccagaa aacatggttc gctctcctct caggccgtgt
     1441 tggaaatccc tacgatgcta ttttctgggg gtgttatccc caccaccctc gtaccgatgg
     1501 gtatctctag actatctttg atgctaaggt ctgcttgcag aattgaccag ccgcaccgcg
     1561 cgatcgtcgc atttcaggcc gggaaaagcg ctccgtaggg ctccgattta attacgcagt
     1621 ccacctagtt ctcgggccct cagcggggaa aactggtttg ggcctcgagg acggccaaac
     1681 cttcgtgaat ggcagaaatc agatctcact ctggacaagg ctttgtcaaa aagcgaaaga
     1741 cctggccctc gttgatggcc ttcatggcgg tcggcaaatc cggctgcccg gtcaagacga
     1801 ttctcaccgt attagaaaaa tgcttagcca cccaggcgag gagccccgtc cctgacgcct
     1861 ggcaagttga gatcgccacg atgttggccc ccattggttg tgtggccatg cctgacgaaa
     1921 tcgtcaaaaa agccttccgc ggcgaacctc tgaaggagaa tgaagtcgcg acctaccaga
     1981 cccatccacg aattgcccac gacttgattt caaaaattcc gcgactggaa caggtggcgc
     2041 ggattgtggc ctaccaggaa aagctttaca acggcggggg ttttccggcg gacctcgtgg
     2101 aaggcgacga catccccttg ggcgcacgca tcatcaaact ggcgtttgac tgggactcgc
     2161 tcattagtgg cggtctcagc ccggaactgg ccctcgctga gatcaccgat cgccgtggtt
     2221 ggtatgatcc caaggtcgtc aatgcgctgc gaaggatcct caatgtccac caggtttgcg
     2281 ttgtccgaga aatgaagatt tctgaccttg tcgatcgact ggtcctcgcc gataacgttt
     2341 actccgtcaa cggcacactg ttgtgcagca agggacaaga ggttaccccg gccattcgtt
     2401 ttcgtctccg aaattacgcg gtcaacgtgg gcatcgcgag acccattcgc tcgttcgttc
     2461 ccactgttgg agcggtgaat gagctgagcg cgctatccga acccaacagc gagtaatcgc
     2521 gcctttcctt tctgcgagcc cccaccaatg ccgcgtgcgt tgctcggtac ccaaaagaaa
     2581 aaagcctgtc aagcagacac ttgcagagcg gtgacgcacg ggcagatggg ccctaactat
     2641 ggcctatgtt caccccctgc atcagggacc ggattggccc agcgtcggac cgttgtcttg
     2701 gggcatactt tgattccagc caagcacgag ctccgttaac cgcctgacca cgtcggggtg
     2761 cttgtcggcc acattctgtg tttcaccggg atcgtttgcc aggttgtaaa gctccggctt
     2821 gctgccatcg tactcacaca ggagcttcca atctccaacg cgaacggcca ggtcggggta
     2881 aggccctttt ccggcgaaga acttgcgatc gggcggacgt cgccagaaga gcggggcctt
     2941 gcgggaatcg ctgctcttcc caagcaacgc cgacgcaagg ttttcaccgt caaaccggac
     3001 gtcggacgga gcggcgatcc cggcaatctc caacaacgaa gggaccaggt ccatcgccga
     3061 aaaaacagag gacttgt
//
LOCUS       DAOPTR010000428         3074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_25367_length_3074_cov_3.349453, whole genome shotgun sequence.
ACCESSION   DAOPTR010000428 DAOPTR010000000
VERSION     DAOPTR010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25367_length_3074_cov_3.349453"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PKI05_08865"
     CDS             <1..363
                     /locus_tag="PKI05_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HOA52356.1"
                     /translation="VEHQAVTTKGQCGYGDVGGVVGATYREQLVELREAMPHTWFLVP
                     GFGAQGATPRDVAAAFDAAGLGAIVNNSRGILFAYRRREFERFGPARWQEAVEAATRD
                     MIEQLRAETPAGRLVGSL"
     gene            complement(500..1087)
                     /locus_tag="PKI05_08870"
     CDS             complement(500..1087)
                     /locus_tag="PKI05_08870"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOA52357.1"
                     /translation="MTTIVLIRPGATNYDLEGRVRGRLDIPMNTQGYQEVAREVETIQ
                     QTGIHLDALYYAPCRPAIETADAIARALDVKAKELDALTNLDYGLWEGMRIEEIRTRQ
                     PKIYRQWQEIPDCICPPEGETIEEAETRVTECLQRLLRRHKNQAVGLVVAEPLASIVK
                     RLITQTPLGDLWKAESRHGQIEILRWDPKPIETAS"
     gene            complement(1084..1794)
                     /gene="rpe"
                     /locus_tag="PKI05_08875"
     CDS             complement(1084..1794)
                     /gene="rpe"
                     /locus_tag="PKI05_08875"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOA52358.1"
                     /translation="MSVHSPLYTDWTLGHVMIGPSLLAADFRYLAREIERLEAAGARF
                     LHLDIMDGHFVPNLSFGIPIVQAVRRCTNLTLDVHLMISNPEAHLERFRDAGADYLTV
                     HIEVAPDPRPLFEQIHRLGACAGVSLNPPTPVETVLPYLNDCELVLVMSVMPGFGGQT
                     FQPEVLAKIRRLRQHSPKGLMISVDGGVNGDTIGHCAAAGATMFIVGTGLLGHNNYHQ
                     RYQELASMVKKSQEKVEL"
     gene            complement(2074..>3074)
                     /locus_tag="PKI05_08880"
     CDS             complement(2074..>3074)
                     /locus_tag="PKI05_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52359.1"
                     /translation="ESVALLNLGFPPEAIKPVKPGQAIIVSKEGIRFEQFGNAPHCAH
                     CFFEWIYFSNVASTLDGRSVYLARKALGEELARLETLPVDDGRTIVVPVPDTSKAAAD
                     AMAFALGVPCLEGLIRNRYTGRTFIDSHMTREERANAKYTPLREVLEGKRVLLVEDSI
                     VRSTTMRVLVHRMRALGRPAEIHIRVACPPIISPCFYGIDMSTMSELFAPRYITNGEL
                     TEAIQARMAKDLGADSLRYLPVEAVARAIGIDPKDLCQACITGSYPTPWGQKLAEAAR
                     ARSLRGETGRTYDAIDTTSERCSRPSPGCDRGLSPAGISSTEGDGPASYGDTSGNR"
BASE COUNT          636 a          904 c          861 g          673 t
ORIGIN      
        1 gtggaacacc aggccgttac gaccaagggg cagtgcggct acggcgatgt cggcggtgtt
       61 gtgggtgcca cctaccgaga acaactggtc gagctccgcg aggccatgcc ccacacatgg
      121 tttcttgtac cgggttttgg ggcacaaggt gcaacacccc gcgacgtggc ggcagccttt
      181 gatgcggccg gtttaggggc gattgttaat aattcccgtg gcatcctctt cgcctatcgg
      241 cggcgggaat ttgaacgctt tggccccgcc cgatggcaag aggccgtcga agccgccacc
      301 cgggatatga tcgaacaact gcgggcagaa acgccggccg gtcggttggt gggtagcttg
      361 tgacagacga tcacaaccga tgacccgatg aaataggtgc tattgggccg tgaccccaaa
      421 cgtccagacc gggcaacggg gtagcctgct cctgcttttg acggatcgtt aagaaggccc
      481 cgatactttc tctggccggt tacgaggctg tttcgattgg cttggggtcc cagcggagga
      541 tttcgatctg gccgtgccgg gactccgcct tccagagatc acctagggga gtctgggtaa
      601 tcagtcgctt aacgatgctt gccagcggct ctgccacaac caatcccacc gcctgattct
      661 tgtggcgacg caagaggcgt tgcaggcatt ccgtaacccg cgtttcggct tcctcgatcg
      721 tctccccttc aggcgggcag atacaatcag ggatttcctg ccactgacgg taaatcttgg
      781 gttgccgtgt gcggatttcc tcaatccgca tcccttccca tagaccgtaa tcgaggttcg
      841 tcagggcgtc caattccttg gccttaacgt ctaacgccct cgcaatagcg tcggccgtct
      901 caatggccgg ccggcaagga gcgtaataga gggcgtccaa atggatgccc gtctgctgga
      961 tggtttccac ctcccgggca acttcctgat aaccctgggt attcatcggg atatcgagcc
     1021 gcccgcggac acgaccctct aggtcgtagt tggtcgcccc ggggcgaatc aggacgatag
     1081 tcgtcatagt tcaacctttt cctgcgattt cttcaccatc gatgcgagct cttggtatcg
     1141 ctggtggtag ttattgtggc ctagcaagcc tgtgccgacg atgaacatcg tcgctcccgc
     1201 cgcggcacaa tgaccgatgg tatctccatt cactccgcca tctactgaaa tcataagtcc
     1261 cttggggcta tgttggcgaa gtcggcgaat tttggccaga acttccggct gaaacgtttg
     1321 tcccccaaaa cccggcataa cactcataac cagtacaagc tcacaatcgt tcaagtaggg
     1381 caataccgtt tctaccgggg tgggcggatt aagcgataca ccggcacacg cacccaagcg
     1441 gtggatttgt tcgaacagcg gtcgcggatc gggagccacc tcgatgtgaa cggttaggta
     1501 gtcggccccg gcatcgcgaa accgctcaag atgggcctcc ggattcgaaa tcatcaaatg
     1561 cacgtcaagc gtcaaattag tgcaccgccg gacggcctgg acgattggga taccaaagct
     1621 caggtttggc acaaagtggc cgtccatgat gtccaagtgc aaaaagcgtg cccccgccgc
     1681 ctctaagcgt tcgatttccc gggctaaata acggaaatcg gccgccagga gtgacggccc
     1741 gatcatgaca tgcccaagcg tccagtccgt atatagtggc gaatgcacac tcatcccaga
     1801 aggaattgcc ttatcatctc acaagacgag tgaccccaat ttttccccag tgtgagatca
     1861 gttcattacc aaggcgaaag tttacctgat ctcaccattt atggtcaaag ggagtcgttt
     1921 cgatttcctt attctcagcc tatttgttca agacccgcga tatttaccaa taaaccacta
     1981 gtttatgcga cgcgaatccg tttgtcattc acccgcttcg tcgatgatgt tatccccata
     2041 aagttgtatc cgggcaaaac cccgccttta gattcacctg ttgccggacg tgtccccata
     2101 gctagctggt ccatcccctt ctgtcgagga aattcccgca ggcgacagac cgcggtcaca
     2161 acccggggat ggccttgaac acctctcgct ggtggtgtcg atcgcgtcat aggtccggcc
     2221 ggtctctccg cgaagcgacc ttgcccgggc tgcctcggct agtttttgcc cccaaggtgt
     2281 tgggtacgaa cccgtaatgc aggcctggca caagtccttg ggatcgattc cgatcgcccg
     2341 ggccacggcc tccacaggca gataccgcag cgagtcggcc ccgaggtcct tcgccatccg
     2401 agcttggatc gcttctgtta actcgccatt tgtgatgtaa cggggggcga acaactcgga
     2461 catggtggac atgtcgatcc cgtagaaaca cggcgagatg atcggcggac atgccaccct
     2521 gatatggatt tctgccggac ggcctaacgc ccgcatgcgg tggacgagaa cccgcatcgt
     2581 tgtggatcgc acaatcgagt cctccaccaa gaggacccgc ttcccttcca gaacttcgcg
     2641 gagcggcgtg tattttgcgt ttgcccgctc ttcccgtgtc atatggctgt cgataaatgt
     2701 ccgtcccgtg taccggttcc ggatcaagcc ctccaagcaa gggacaccca aggcaaatgc
     2761 catggcgtcg gcggctgcct tgctggtatc ggggacgggc acgacgatcg tccggccgtc
     2821 atcgacgggg agtgtctcga gccgggccag ttcttcgccc agagccttgc gggcgaggta
     2881 aacacttcgg ccatccagag tgctcgccac attggaaaaa tagatccact caaaaaagca
     2941 gtgggcacaa tggggggcgt tcccaaactg ctcaaagcgg atgccttcct tggagacgat
     3001 gatggcttga ccgggtttga cgggtttgat ggcctccggc gggaatccca ggttcaaaag
     3061 cgcgacactt tcgc
//
LOCUS       DAOPTR010000429         3073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_25374_length_3073_cov_3.769384, whole genome shotgun sequence.
ACCESSION   DAOPTR010000429 DAOPTR010000000
VERSION     DAOPTR010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25374_length_3073_cov_3.769384"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PKI05_08885"
     CDS             complement(<1..299)
                     /locus_tag="PKI05_08885"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOA52360.1"
                     /translation="MNQEEIKALAEFFRLSPAEFEERFVRTIGKAKSLIEQPNGDCVF
                     LHPLTRKCLVYELRPRQCRSFPFWSSILKTKRTWEEICRTCPGCGQGPLHSLEEI"
     gene            complement(444..>3073)
                     /locus_tag="PKI05_08890"
     CDS             complement(444..>3073)
                     /locus_tag="PKI05_08890"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOA52361.1"
                     /translation="RALRQYEDLPKTPQVQVKGCGYHIDNVLSNGCLDFGKLLVGSEG
                     TLALVLEMTFETLPTPPARSLTLLLFPQLEPAFDAAIEILRFRPTICDLMDRRHLSLA
                     RESDEHLEQLVPDNTEAAILVEIEGQTLRETYNELRTILDSLRNYHRFEFSARWANSE
                     DEIASFCRLARITQPVARRASHRFRPLPVADNISIEPERMATCVSQLQAILRKHDVAS
                     SVYCHVGDGVIYLQPFFDTQDRHLFRKMKQVADEVYGYVLSVGGGIGPTLGCGLARTP
                     YMKQQWGHLYPIFEKIKKAFDPLNILNPGKIIETPDTGDIWKLARGRLFVGFEAPSSE
                     DDGNEDGAGFQRTGVHTTQGATSLPETQSRGERQDDIPAPAASTAGVIDFGDRAAAEI
                     DSQHYSYSPPSELTLNAPPMAQQDHIPGRLDDRSENLPRILESQLDWQPDLVANAVSR
                     CSSCGDCRTQDHRVRMCPLFRALPWEECAPRSKSSLMRAVLTETLPLQSLTLQDFREI
                     CDLCVHCHSCRIECSAQVDIPLLASEAKAAYTLAHGSAFADWLLNRIDGLASLLSRFA
                     PLVNWGLANRQVRWLLEKLTGISQVRKLPRLHAVSFLHRAARKKLHRLDRLPGRKVVY
                     FVDVYANWFDPALAEALVAILAHHGISVFVPLQQRSAGTAAIASGEMNLARRHARLNV
                     AILAEAVRQGCEIVTTEPAAALTLIHEYPQLLQDEDSQLVAKHTHDAGEFLLGLHRDG
                     QLRTDFQNLPLRLGYHLPCRLRALGVGQPGRELLELIPGVTVEGLPNTCSGMAGMFGI
                     KAKNFRTSLRLGWSIISHLRDPAIQAASTECSACRIQLEQGTTKPTLHPIKVLAAAYG
                     LWPGGLQRLLRPGSSFVLS"
BASE COUNT          710 a          835 c          865 g          663 t
ORIGIN      
        1 tcgatctcct ctaatgaatg cagaggccct tggccgcatc ccgggcaggt ccggcagatt
       61 tcctcccaag tgcgctttgt tttcaaaatg gatgaccaaa aggggaaact ccggcattgt
      121 cgtgggcgga gttcatagac caagcatttt cgggtcaggg ggtggagaaa gacgcaatca
      181 ccgttgggct gttcgatgag acttttcgct ttcccaatgg tgcggacaaa ccgctcttcg
      241 aattccgccg gggagagtct gaagaactcc gcaagggcct tgatctcttc ttggttcaca
      301 tagacatatc ccggctgacc ggaacaacag tacccacaac cggtacattc aaaccgcagg
      361 ccttcatggt accagggcaa ggctgttttt ttgctgacac gttgcatagt caaagatttt
      421 tctaagcggg aactaccttg gacttaggac aacacaaatg atgaccccgg tcgcaacaat
      481 cgctgtaacc cacccggcca caaaccgtag gccgccgcca agaccttaat ggggtgaagc
      541 gtcggcttgg ttgtgccctg ctcaagctga atccgacatg cgctgcattc cgtgctggcg
      601 gcctggatgg cgggatcgcg gaggtgactg atgatcgacc agccaagtcg caggctggtg
      661 cgaaagtttt tcgccttgat tccaaacatc ccagccatcc ctgagcacgt gttgggaagt
      721 ccctcgacgg tcacgccggg gatcaactcg agcaattcac gtcctggttg gcccacccca
      781 agggctcgca aacgacatgg caagtggtag ccaagccgaa ggggtagatt ctgaaaatcc
      841 gtccgcagtt gtccatcacg atgaagaccc agcaggaatt ctccggcatc gtgggtgtgc
      901 ttggcaacca gttgggagtc ctcgtcttgg agaagctggg gatactcgtg aatcagcgtc
      961 agggcggcgg ccggttccgt cgttacaatt tcacatccct gccgtacggc ctcggcgagg
     1021 attgccacgt tcagccgcgc gtgccgtcgc gccagattca tttcgccgga ggcaatcgcg
     1081 gccgttcctg ccgacctctg ctgtagtggg acgaagacgg aaatcccatg atgggccagg
     1141 attgcaacaa gggcctcggc aagtgctgga tcaaaccagt tcgcataaac atccacaaag
     1201 taaacgacct tccttccggg aagccggtca aggcggtgca gttttttgcg agcagcgcgg
     1261 tggagaaaac tgacagcatg tagccgcggt aactttcgga cttgggaaat accggtcaac
     1321 ttctcgagca gccagcggac ttgccggttc gccagacccc aattgacaag cggggcaaat
     1381 cgggagagca gggaggccaa tccgtcaatc cgattcagca gccaatcagc gaaggcgctt
     1441 ccgtgggcca gcgtgtatgc cgccttggct tcactggcca gcagaggaat atccacctgg
     1501 gcagagcact caattcggca cgaatggcag tgcacacaga gatcacaaat ctcgcgaaaa
     1561 tcctgcaacg ttaatgactg gaggggaaga gtctcagtga gtactgcccg catcagactg
     1621 gactttgatc gtggggcaca ctcttcccaa ggcaatgccc gaaacaaggg acacatcctt
     1681 accctgtggt cctgcgtccg gcaatcgcca cagctcgagc accgcgacac ggcgtttgca
     1741 accaagtctg gttgccaatc caattgcgat tccaaaatcc tgggcaaatt ctctgaacgg
     1801 tcatccagtc gccctgggat gtgatcctgt tgcgccatgg gcggcgcatt tagagttaac
     1861 tcggacgggg ggctatagga ataatgttga gagtctatct cggccgcggc acgatcccca
     1921 aagtctatta cgccagctgt tgatgcggcc ggcgcaggga tatcatcttg tcgctcacca
     1981 cgggattgcg tttctgggag agatgtcgca ccttgggtcg tgtgaactcc ggtccgctgg
     2041 aagcccgcgc catcttcatt gccgtcgtct tccgaactag gagcctcaaa cccaacaaac
     2101 aaacggcctc ttgccaattt ccaaatgtcg ccggtatcag gggtctcaat gatttttcct
     2161 ggattaagaa tgttaagcgg gtcgaaggcc tttttgattt tctcgaaaat gggataaaga
     2221 tgcccccact gttgcttcat gtagggtgtt cgagctaagc cgcacccaag agtcgggcca
     2281 atcccgcctc cgacactcaa aacgtaacca tagacttcgt ccgccacctg tttcattttc
     2341 cggaagaggt gacggtcttg tgtatcaaaa aatggctgga ggtagatgac gccatcaccc
     2401 acgtggcagt aaacggaact ggcaacatcg tgctttcgca ggattgcttg aagctgggaa
     2461 acacaggttg ccatcctttc cggttctatg gagatattgt cggcgacggg cagaggtcga
     2521 aatcggtgac ttgcacgacg ggctaccggt tgcgtaatgc gagcgaggcg gcagaagctg
     2581 gcaatctcat cttcgctatt ggcccagcgt gcggaaaact caaaacgatg gtaatttcgc
     2641 agggaatcta agatagtgcg cagctcattg tatgtctcgc gcaaggtctg cccttcgatc
     2701 tctaccaaaa tggccgcctc ggtgttatca gggaccaact gctcgaggtg ttcatcggat
     2761 tcccgagcca gactcaaatg ccgccgatcc atgaggtcgc atatggtggg ccgaaagcgg
     2821 aggatctcaa tcgcagcatc gaatgccggc tctagctgcg ggaataataa gagtgttaag
     2881 gaacgggcag gtggtgtagg cagcgtttcg aaggtcatct ccaagacaag ggccagcgta
     2941 ccttcggagc ccacaaggag tttgccaaag tcgaggcaac cgttagaaag aacgttgtct
     3001 atgtggtagc cgcatccctt tacctgcacc tgtggcgtct ttggcaaatc ctcatattgc
     3061 cgaagagctc ggg
//
LOCUS       DAOPTR010000430         3065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_25483_length_3065_cov_3.288372, whole genome shotgun sequence.
ACCESSION   DAOPTR010000430 DAOPTR010000000
VERSION     DAOPTR010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25483_length_3065_cov_3.288372"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..1311)
                     /locus_tag="PKI05_08895"
     CDS             complement(73..1311)
                     /locus_tag="PKI05_08895"
                     /inference="COORDINATES: protein motif:HMM:NF017666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HOA52362.1"
                     /translation="MDVALQRLPMELIDDHPDNPRLAFRQDVIDAIAANLNGQYPQKH
                     AIHVRPAGGRFQIVSGHHRIRAARQAGLKEVWAWVEPLDDEQALMELVLSNNQGELSP
                     LEIGLHCLNAVPSGDKGRGKLGGLREYARRIGRDHTTVLRYRHAAEVLLAAEKMVSQG
                     TIFCDKAKHLATIHRLPQDRWAEFVDWVAQANPTVAEVEERVAKALRPATSPQPFQDP
                     QPEEPSIVPHRDTLWEPSLPEPEEPRPLPHVAFNSGDSEWYTPWEFIERAVAVMGGVD
                     LDPASTPVANEVVKAKKFYTAEDNGLAHVWRGRVFLNPPYAQPLVQQFADKLVQHVQE
                     GHVTEAVVLVNNATETRWFQALLAAAQAVCFPAGRVKFWHPEKIAAPLQGQAVIYFGP
                     QVDRFQQAFADLGRVCHVAR"
     gene            complement(1327..1635)
                     /locus_tag="PKI05_08900"
     CDS             complement(1327..1635)
                     /locus_tag="PKI05_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52363.1"
                     /translation="MRVDYRISEFTWVSEWVCFEHTGYARWKAEQWWRRRSPDPVPET
                     AERAVEIAEAGGVAWTEKIVVRTVAGEKYDRIVGYRLGPMPEPVYSTAAECDLDDVPF
                     "
     gene            1672..1941
                     /locus_tag="PKI05_08905"
     CDS             1672..1941
                     /locus_tag="PKI05_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52364.1"
                     /translation="MDAVTDVKDLVLGIGDLAREHPGGAGPGFVLLAFRRRELVAALG
                     ARGIASGDDRVTVGAFLGRNTLSHSKSQVFHGDYDTASSARWRIG"
     gene            2114..2515
                     /locus_tag="PKI05_08910"
     CDS             2114..2515
                     /locus_tag="PKI05_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52365.1"
                     /translation="MWRLSFVLVGMLALSANEVWAQWSSRPNILGGYDVSGPQGQFWS
                     SSRNIFGGLDWHGPGGFWSSRPNILGGYNVSGPQGQFWSSSRNIFGGLDWHGPGGFWS
                     SRPNILGGYNVSGPQGQFWSSSRNIFGGLDW"
     gene            complement(2542..>3065)
                     /locus_tag="PKI05_08915"
     CDS             complement(2542..>3065)
                     /locus_tag="PKI05_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA52366.1"
                     /translation="PKGIVYGPPGVGKTSFGASADRPLIIDCENGAAHVQCHRTPYLA
                     SWPEIQPWLDGLAAGGHEYQTVVIDSIDWMFRRIEEHVAGVDGTPTGMKQTLNRSHGG
                     YGNGRQVLKNYVYQYLLPILDRLVNSGVAVLLLAHAARRSLTTIDGIELEKYAPEIHP
                     DLANTMIEWSDFR"
BASE COUNT          526 a          902 c          998 g          639 t
ORIGIN      
        1 tcgatcttgg gccgtttctt ctggttgaag catccctgct tcgtgcaatc ccatcggagt
       61 ggattggccc cgttatcgcg ccacatggca caccctcccc aaatctgcga aggcttgctg
      121 gaaccggtcc acctgagggc caaagtagat cacggcctgg ccttgcaagg gggcggcgat
      181 cttctccgga tgccagaact tcacccggcc ggcgggaaag cagaccgctt gggcggcggc
      241 caagagcgcc tggaaccagc gggtttcggt ggcgttattg accagcacca ccgcttcggt
      301 cacgtgcccc tcctggacgt gttggacgag tttgtcggcg aactgctgca ccagcggctg
      361 tgcgtagggc ggattgagaa acacccgccc acgccaaacg tgtgcaagcc cattgtcttc
      421 ggcagtgtag aactttttcg ccttcacgac ttcattggca accggagttg atgccggatc
      481 aaggtcgacg ccgcccatga cggccacggc ccgctcgatg aactcccacg gcgtgtacca
      541 ctcgctgtcg ccgctgttga aggcgacgtg gggcagaggg cgtggctcct cgggctccgg
      601 aaggctgggc tcccagaggg tgtcccggtg cggcactatg gatggttcct ctggttgagg
      661 gtcttggaag ggttgtgggc ttgtggcagg acggagagcc ttggccacac gctcttccac
      721 ctcggccacc gtagggttgg cctgggcgac ccaatcgacg aattccgccc acctgtcttg
      781 gggcagcctg tggatggtgg ctagatgttt ggctttgtcg cagaaaatgg tgccctggga
      841 caccattttt tcagcagcaa ggagcacctc cgcagcatgg cgatagcgca aaaccgtggt
      901 gtggtctctg ccgatgcgtc tagcgtactc tcgaaggccg cctaatttac ctctcccctt
      961 gtctccggac ggcaccgcgt tcaaacaatg caagccgatc tccaagggcg acagttcccc
     1021 ctggttgttg gacaatacca gctccatcag ggcttgctca tcatccagcg gttccaccca
     1081 ggcccagact tctttcagac cagcttgcct tgcggcacgg atacgatggt ggccggaaac
     1141 aatctgaaac cgcccccccg caggtcggac gtggatggcg tgcttctgtg gatactggcc
     1201 attgagattc gcggcgatgg cgtcaatcac gtcctggcga aacgccaggc gcgggttgtc
     1261 ggggtggtcg tctatcagct ccatgggcaa gcgttgcagg gctacatcca ttgcgttacc
     1321 tcacaatcag aacggaacgt cgtccaggtc acactcagcg gcggtgctat acaccggctc
     1381 cggcatcggg cccagcctgt agcccacgat gcggtcgtac ttctcgcccg ctaccgtgcg
     1441 cacgacgatc ttctcggtcc aggccacgcc gcccgcctcg gcgatttcca cagcccgttc
     1501 ggccgtctcc ggcaccgggt cgggggaacg gcgccgccac cactgttcgg ccttccagcg
     1561 ggcgtagccg gtgtgctcaa agcagaccca ctcggagacc caagtaaact cgctgatgcg
     1621 gtagtccacc cgcatggtct tgggggcgtc cggcggggca tcccgtttgg tgtggacgct
     1681 gtaacggacg tcaaagacct cgtactcggt atcggtgacc tggccagaga gcaccccggc
     1741 ggtgctggcc cgggcttcgt gcttctggcg ttccggcggc gggagcttgt agccgcactc
     1801 ggggcacgtg gcatagccag cggcgatgac cgcgtgacag tcggggcatt ccttggccgg
     1861 aatacgctgt cacattccaa gtctcaagtg ttccacggcg attacgacac ggcttcctca
     1921 gcgcgctggc gaatcggttg aagaggccct tgggcaaatg ccacgctatc tggggacagg
     1981 tcttgggcgg acatacaaaa cacatctacc acccggtggc ggggcttgac tttgcgtttt
     2041 ttttgttatg ctgaacttaa gttgggtctc gtggaaagac gttccaagtt tcctgaccga
     2101 agggagaagt aagatgtggc ggttgagctt tgtgttggtg ggcatgttgg cccttagtgc
     2161 caacgaggtt tgggcacagt ggagcagccg gccgaacatt ttaggcggct atgacgtgtc
     2221 tgggccgcag gggcagttct ggtctagcag ccggaacatc tttggcgggt tggactggca
     2281 cggaccgggt ggcttttgga gcagccggcc gaacattttg ggcggctata atgtgtctgg
     2341 gccgcagggg cagttctggt ctagcagccg gaacatcttt ggcgggttgg actggcacgg
     2401 accgggtggc ttttggagca gccggccgaa cattttaggc ggctataatg tgtctgggcc
     2461 gcaggggcag ttctggtcga gcagccggaa catcttcggg ggcttggact ggtgaacacc
     2521 tgctaccgag gctcttagtt gttacctgaa gtccgaccat tcgatcatcg tgttggccag
     2581 gtcggggtgg atctcggggg cgtacttctc cagttcgatg ccatcgatcg tggtgagcga
     2641 acggcgggcg gcgtgggcca acaagagcac ggccacgccc gagttgacta gccggtctaa
     2701 gatgggcaag aggtactggt agacgtagtt cttgagtacc tgccggccat tgccgtagcc
     2761 gccgtggctg cgattgagcg tctgcttcat gccggtgggc gtgccgtcca cgccggccac
     2821 gtgctcctca attcgccgga acatccaatc aatcgagtcg atcacgacgg tctggtactc
     2881 gtggccgccg gcggccaggc catcgagcca gggctggatc tccggccaac tggccaggta
     2941 gggcgtgcgg tggcactgca cgtgggctgc accgttttcg cagtcgatga tgagcgggcg
     3001 gtcggccgag gcgccaaacg acgtcttgcc gacgcccggg ggtccgtaca cgatcccctt
     3061 cgggg
//
LOCUS       DAOPTR010000431         3062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_25529_length_3062_cov_3.087130, whole genome shotgun sequence.
ACCESSION   DAOPTR010000431 DAOPTR010000000
VERSION     DAOPTR010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25529_length_3062_cov_3.087130"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="PKI05_08920"
     CDS             complement(<1..1070)
                     /locus_tag="PKI05_08920"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOA52367.1"
                     /translation="MAVTRNVEEDSSGEGLPTIVKRLEFPDAVSLVELSNGLTIIVQE
                     NHSVPVATVRCYVKYTGSIYEAEYLGAGLSHVLEHVVSGGTTTKRTEREIAAMIDRFG
                     GKSNAYTSTNFTCYYIDCPSKNVNEAIELIADAMQRVAFEPSEFERELKVVKQELADG
                     EVNRSRVLWKLLNQTLYLQHPARHPVIGYLDVLNRTSNEAIINFYKSRYVPNNQIFVV
                     VGDVTVDEIVKSIAKQYQGSKRAPETYLPLQEEPPQLTPRRVVREMEGDVVDFVIAWP
                     TVKLSDPDLYPLDVAADLLSRGDSSRLVRRLQREQPLALQVDAVSATPEYVRGWFGVM
                     ARCLPDRFEEVRNIIFQEITRLR"
     gene            complement(1567..2460)
                     /gene="thyX"
                     /locus_tag="PKI05_08925"
     CDS             complement(1567..2460)
                     /gene="thyX"
                     /locus_tag="PKI05_08925"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOA52368.1"
                     /translation="MSTNISVVEQLRWRKLPVLDDGFVCLVDVMGDDSAIVQAARVSY
                     GAGTKKVSDDRTLIRYLMRHRHTTPFEMAEVKLLVRVPMDIWRQWIRHRTASVNEYST
                     RYSIAIDTAHRTPPDKWRKQSTTNRQGSEGFLDLQTGEELSREERELQEKARQVYQHR
                     LDLGVAREQARKDLPLSTYTEAYWKIDLHNLLHFLELRMDRHAQWEIAQYARVIGYEI
                     VAPLFPMTWEAFVDYRLQGLVLSRLDCEVIARLVAQGSLPASERAFLEAQDPAWRGLT
                     RCRERDECWEKLKRLGIVKDP"
     gene            complement(2535..2837)
                     /locus_tag="PKI05_08930"
     CDS             complement(2535..2837)
                     /locus_tag="PKI05_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007333356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52369.1"
                     /translation="MRMKDNKPSRSAVNRTPYQEGIIRRYYENRDNLMIQKLGDLVGE
                     LYLSEGKARARIWKRITAALRNLSVPEARIEHLVKSDNPEFVAKILEELQTRGQVD"
BASE COUNT          709 a          878 c          743 g          732 t
ORIGIN      
        1 cgtagccggg taatctcctg gaaaatgatg ttgcggactt cctcaaaacg atcgggcagg
       61 cagcgagcca taacaccaaa ccacccccga acatattccg gggttgcact cactgcatcg
      121 acctgcaaag cgagcggttg ctctcgctga agccgccgaa ccaatcgcga cgaatcaccc
      181 cgagatagca aatccgccgc aacatcgagc ggatacagat cgggatcgga gagtttcacc
      241 gtcggccagg caatcacgaa gtcaacgaca tcaccttcca tctcgcgcac tacccgccgc
      301 ggcgtcaatt gtggtggttc ctcttgaagt ggcaggtacg tctcgggggc tcgcttactc
      361 ccctggtact gcttggcgat acttttcaca atttcgtcaa ccgtaacgtc gccgacaacc
      421 acgaaaattt gattgttagg aacgtaacga gacttataaa aattgatgat cgcctcgttg
      481 ctcgttcggt ttaacacatc gagataaccg ataacgggat gtcgggcggg atgctggagg
      541 tacaaggttt gattcaagag tttccacaac acacgagacc gattgacctc gccgtcggca
      601 agttcctgct taaccacctt aagctcacgt tcgaattcgg aaggctcaaa cgccacgcgc
      661 tgcatcgcgt cagctatcag ctcgatcgcc tcgttgacgt tcttagacgg gcagtcaatg
      721 taatagcaag taaaatttgt tgaggtgtag gcgttgctct tgccgccaaa acggtcgatc
      781 atggcagcaa tttcgcgctc ggtgcgcttg gtggttgtcc caccagagac aacatgctcc
      841 aagacgtgac taagaccagc accgagatat tccgcttcgt aaatgctgcc agtgtatttc
      901 acataacagc gaacggtcgc caccgggacc gaatgatttt cctgaacaat gatcgtcaag
      961 ccgttcgaga gttcaacgag ggagaccgcg tccggaaact ccagccgctt gacaatagtc
     1021 ggcaaccctt ctcccgagga atcctcctcc acgttccttg tcactgccat acccatcgag
     1081 cctaacgaca taagccaccc caacaaacaa aaaacaaacg ctccatgtaa gtgatgattt
     1141 gtcgctctcc tacgcattgc ggaacactcc ttctgatcat tattccgttc cggccagtgg
     1201 ttgctgtgac aatcggatca tcagagacga attcacgccc gtcgtaagtg tttcccttgg
     1261 atgcatacag aggaaccgcc aactgtgcag caaaaaacgg agcaacacca cttccggcct
     1321 ctatcactgg accacattca tgtagcttca ctattatggc aattctttgc agatggtcca
     1381 gggggcaata cgttggccga gtaactccgg cggaaagtta cattatctgc caatggcggg
     1441 agaaagatga gaatttaata tatcacgcta ctgcggacta gtgatcgggc agtgccaggg
     1501 atggggcaat gccacagcag ggccatcaga gaccctcttt tgtaacttct tcgtcgagtt
     1561 tataggctat ggatccttca cgatccctag gcgtttgagt ttctcccaac actcgtctcg
     1621 ctcgcggcaa cgtgttagtc cccgccaggc tgggtcctga gcttcgagaa aggctctctc
     1681 actcgctgga agagagcctt gagcgaccag gcgagcaatc acctcgcagt ccagacgcga
     1741 tagcaccagc ccttgcaatc tatagtccac aaatgcttcc catgtcattg ggaatagcgg
     1801 agcgacgatt tcgtagccga tcacacgcgc atattgggcg atttcccatt gcgcgtgacg
     1861 atccatccgc aattcaagaa agtggagcag attgtgcaga tcgatcttcc agtaagcctc
     1921 ggtataggtc gaaagcggca agtctttcct tgcctgttcc cgtgcgactc ctaaatcgag
     1981 gcgatgttgg tagacctgcc gggccttttc ctgcaactct ctttcctcgc ggctaagctc
     2041 ttctcccgtt tgtaggtcca aaaacccttc gctgccctga cggttggttg tggactgctt
     2101 tcgccactta tccggaggcg ttctgtgggc ggtatcgatg gcgatcgaat accgtgtgct
     2161 gtattcattc acagaggccg tgcgatggcg gatccactga cgccaaatgt ccataggtac
     2221 ccgcacaagg agcttgacct cggccatttc gaacggggtc gtgtggcggt gacgcatcaa
     2281 atagcggatc aacgtccgat cgtcggaaac tttcttggtc cccgcgccgt agctaacacg
     2341 ggccgcctgg acaatcgctg agtcatcgcc catgacatcc accaggcaca caaagccgtc
     2401 gtccagcacg ggcagctttc gccatcgcaa ttgttcaaca acggaaatat ttgtggacat
     2461 actcccaaat cttcactttt ctggacctag cagtcctgaa tgggcaaccg cgacacaagt
     2521 ggggatataa ccttttaatc cacttggcca cgggtttgaa gttcctccag aatcttggcg
     2581 acaaactccg ggttatcgga cttcaccagg tgctcgattc tcgcctctgg gacgcttaga
     2641 tttcgaagtg ccgcggtaat ccgcttccag atacgcgctc ttgcctttcc ctcactcaga
     2701 tagagttcgc caaccagatc tcctagcttc tggatcatca gattgtcccg gttctcgtaa
     2761 taccgccgga tgatcccttc ttggtaaggc gtacgattta cagcagagcg cgacggcttg
     2821 ttgtccttca tcctcatatt gcttcccatc ttaaacaatg ctggtctcaa acctgcagtt
     2881 cgttactgcc acgatccata ctagccatta tcgtcaaacc gccgcggcat cctttgtgag
     2941 caattcttgg ggaagtcacc gtcaccccaa ccacctgccg attctcctct gtgttcatta
     3001 tgtcttttgg tcgtcaactc ttagagtagg ccccctagga tcgctattgt gtctggcgat
     3061 ga
//
LOCUS       DAOPTR010000432         3058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_25573_length_3058_cov_2.593740, whole genome shotgun sequence.
ACCESSION   DAOPTR010000432 DAOPTR010000000
VERSION     DAOPTR010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25573_length_3058_cov_2.593740"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(378..641)
                     /locus_tag="PKI05_08935"
     CDS             complement(378..641)
                     /locus_tag="PKI05_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52370.1"
                     /translation="MAIALTLLRKHLRNGQSVILWDSERKPLVEMVYCTVKGEKFHSP
                     VVISWDASRIPKTRAVRQIIMDIEDEYPGYYLDVIMDNLVTTA"
     gene            complement(1112..2077)
                     /locus_tag="PKI05_08940"
     CDS             complement(1112..2077)
                     /locus_tag="PKI05_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52371.1"
                     /translation="MRCDDGEYERTGTIPLAIPERGKSAHWLLVNAYSDEDIAKAVEG
                     RSHIRALLIDIGPDVLRTLRDDKPTVSDEGLAIVTKLPDLESLCITGKGELTGDFLAY
                     VSALCNLQVLDLSGFPAITDESLAHLKELHGLRVLSMRNCGVHGPGLAHLVTLPALES
                     LDLSGNPLDDLNKSELSRFQSLQALFLDDTPVRDADLRHIARLERLRALGLGNTGISN
                     QGLRYIANLKDLEFLNLSGTGITDEGLHWLSKLATLQKLDLSATRISDNGIPQLTSLR
                     NLQSLDLRGTEVTDFGRKQLKRIAEFRTVELDMPVFWMAAEELSE"
     gene            complement(2241..>3058)
                     /locus_tag="PKI05_08945"
     CDS             complement(2241..>3058)
                     /locus_tag="PKI05_08945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOA52372.1"
                     /translation="AVDQILAEENVDNGADETVQWTVTDHLNTVRDIAKFDPQTGATT
                     VVNHLVYDAFGNVTSDSNPGVDSLFLFTGRPFDSDTRLQNNLNRWYDASVGRWLSEDP
                     IGFAGGDGNLYRYVGNRSLTAVDPEGLTLWYRGTLTYRVIQPIRTISVVLPAKGSSGL
                     SKERIQKLVEDATREWAKNDGKIIFHIDKRCPAGTIPGPGQRIRVAQQFEGELRIFVK
                     LSRRFFGLFGDWTAVIVTDPRKEDDQWSLGTVLFLAVYQKEFYGECGPCCEKT"
BASE COUNT          757 a          866 c          702 g          733 t
ORIGIN      
        1 tggtaaatac aaggcgacta tagctgacta agaatgcaga gtaatcaaat aataaaacat
       61 gccctatcgc cggattactc tccgacgtga ccttgtcgaa ggcgtcgtag atgaggtggt
      121 tgaccacgat cgtcgcccct tgcctccgcc acatctgggt tgtcgggtct tgcgaccacg
      181 ccggaaaccg tcagcgggtc gaagatcgca atgtccggca cggtattcaa cgtggtcggt
      241 cagagttgat cgaacatgcc ccaacgattc cctcagcggt tctatcatca aggaggtcat
      301 ctcccccata tccacggttg acgtaccttc ctgtagctta tgtcgaatga gggattagtc
      361 cacatgtaac cggtgtgtta tgcggtggtc accaagttgt ccatgatgac atctaagtaa
      421 taacctggat actcatcctc aatatccatt atgatctggc ggacagcgcg agtcttcgga
      481 attcgagacg catcccagct aatcaccacg ggcgagtgaa acttttcccc ttttacggtg
      541 caatacacca tttccacaag tggcttcctt tcgctatccc acaggatgac actttgccca
      601 ttgcgcagat gctttcgaag aagtgtaagc gcaatagcca tcctacattc ctcttggaat
      661 tcgtgaacca atcgattcaa tgcataactt tcgaataggt ttaaaggacg cgcctgaatc
      721 tccagaagga gggtcgttcc cgtgctgaat agatgccaac gagttaattg tgcgtcgacg
      781 cgcgcaacgg ttctgcctga tcgcgatacg atgtcttgac gaaacgccgc actacctagc
      841 gtctctaaca gttcttcttg ttcttcctgc gtaagggcgg gcgccggaaa cggggctata
      901 tcgtaagtag tgctcacggc ccgtgggtaa aaaacggcga ccccaatgac aacaccccct
      961 acaggtgctg caatgattac gcccagtaag acgcgcttga ctgccgtatt gctcattcac
     1021 acctcacttc ttcgaaacac aaacattccc aactgcccca gctctttggc atctattgaa
     1081 gagtgccgaa taagcgagtt gttgacacac actactcgga tagttcttct gcggccatcc
     1141 aaaaaaccgg catgtctaac tccacggtcc taaattcagc tatccgctta agctgtttac
     1201 gcccgaaatc ggtgacctca gtccctcgca aatcgaggga ttgtaagttt ctcaggctag
     1261 taagctgcgg aattccgttg tcagatatac gcgtagcgct caaatctaac ttctgaaggg
     1321 ttgcgagttt gcttaaccaa tgcaagccct catccgttat gcctgtgccg gataaattta
     1381 aaaattccag atctttcaaa ttcgctatat atcgcaagcc ttggttacta atgccagtat
     1441 ttccgagtcc caacgcgcga agccgctcaa ggcgagcgat atgccgcaag tccgcatcac
     1501 gcacgggcgt gtcgtccaaa aaaagagcct gcaagctctg aaacctacta agttcggatt
     1561 tgttcagatc gtccagcgga ttccctgaga gatcaagcga ttccaaagcc ggtaacgtca
     1621 ccaagtgtgc taacccgggg ccgtgaacgc cgcaattccg catggaaaga acacggagcc
     1681 cgtgcaattc tttcaaatgt gcaagagatt catcagtaat ggctggaaaa ccactgagat
     1741 cgagtacctg cagattacat aaagcagaga cgtacgccag aaagtcaccg gtcaactccc
     1801 ctttccccgt tatacatagg gattccagat ctggaagttt tgtgacaatc gccagtcctt
     1861 catcgctaac tgtgggctta tcgtctcgca aggttcgtag aacatcaggg ccgatgtcga
     1921 tgagcaaagc acggatgtgt gaccggccct ccaccgcctt cgcgatgtcc tcatcggaat
     1981 aggcattgac aagaagccaa tgtgcggact tacctctttc cggaatcgcc agcggtatcg
     2041 tgccagtccg ttcatactcg ccgtcgtcac atcgcaagca gccgcaaccc atgcaagcga
     2101 tgaatccgac agagagtgta atccctaaaa atccctttcc agtccgtgtg cacatgggat
     2161 cgcaattctc ttccttcaaa tcttcctgaa cttcagatgt caacgactaa acggcaaaaa
     2221 acggccatcc taccacgagc ctaggttttc tcgcaacacg gtccacattc gccgtagaat
     2281 tctttttgat aaacagctag aaataacacc gtacccaaag accactgatc gtcttccttg
     2341 cgtggatcag tcacaatcac ggcagtccaa tccccaaata acccaaagaa tcgacggctc
     2401 agctttacga aaatccggag ctcgccctca aattgttgtg caacccggat acgttgacca
     2461 ggcccaggta tcgtgccggc cggacatcgt ttatcgatgt ggaaaataat ttttccgtcg
     2521 ttttttgccc attctcgggt tgcatcctcg acgagcttct gtattctctc cttcgagagg
     2581 ccagaggagc ccttcgccgg aagaaccaca cttatcgttc ttatgggctg gatcactcgg
     2641 tatgtcagcg tacctcgata ccatagcgtt aacccttccg ggtcaacggc tgttaagctt
     2701 ctattcccca cataccgata caaattccca tccccgccgg cgaagccgat cgggtcctcg
     2761 ctcagccagc ggccgaccga agcatcgtac cagcggttga ggttgttctg aagtcgcgtg
     2821 tcactatcaa atggtctacc cgtgaataag aagagactat ccacccccgg attactatcc
     2881 gacgtcacgt tgccgaaggc gtcatagacc aggtggttga ccacggtggt ggcccccgtc
     2941 tgcgggtcga acttggcgat atcccgcacc gtgttcaagt ggtccgtcac ggtccactgt
     3001 actgtttcat cagccccatt gtcaacattt tcctcggcca aaatctggtc caccgccg
//
LOCUS       DAOPTR010000433         3055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_25622_length_3055_cov_3.605000, whole genome shotgun sequence.
ACCESSION   DAOPTR010000433 DAOPTR010000000
VERSION     DAOPTR010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25622_length_3055_cov_3.605000"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PKI05_08950"
     CDS             <1..369
                     /locus_tag="PKI05_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52373.1"
                     /translation="AQRILDFFRRLPQSHVGQVIYTQYEQLLANRGEVERVIRFLGCD
                     PGTFPWDRFENLPVRGSSFYRGNRQELHWQPVEKGKDFQPVGRWKHWPWWVRWQFRRI
                     AGREFEALGYTWSAGTESIR"
     gene            379..1332
                     /locus_tag="PKI05_08955"
     CDS             379..1332
                     /locus_tag="PKI05_08955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOA52374.1"
                     /translation="MAETSGPPREFPRISVIIPVFNGERFLGEAIKSALSQTLPPYEI
                     LVIDDGSTDRSAQLAESFGPPVRVIRQENRGEAAARNRGIDAAQGDWIAFLDCDDVWK
                     PEKLALQAALLSPAVNCVHTNFFYFGASQGTVDVSDVPPATRYSLGYVARRNPFRISS
                     LLVRRDLDIRFPEWTQDGEDLIYFLELARKCEIRLVREFVTGYRVHAKGQSSRPDMLV
                     RRFHALQQFLEGVADLPVAEKDLVQREYLALMAEQATLAKYRRNWQEYWALRRFLASW
                     KGPLPAEAKPVLREFIFPPLVYRVKDWFDALTRCDADSGSG"
     gene            1339..2547
                     /locus_tag="PKI05_08960"
     CDS             1339..2547
                     /locus_tag="PKI05_08960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA52375.1"
                     /translation="MALQTPAREVIAHESRQLRIFIAAHSGQQGGAELCLDVFLAHLP
                     RARYKATVLFGCEGPMVQAAAEKGYPTQVVPFVWWFGYEPCSWYWRNLLKSPFRVARL
                     ARLLRQESFDVVYTNSAVIFEAALAARIAHCPHLWHVHEILRPQSWRSWLPIRTICRL
                     IDRWSDLIVFESQAARNIFASLHQPKTKTDAVSNPVRFLTDIEPSHDPAVIRNTLGLP
                     QDALIVVWIGQFIPRKNPQLAIEGFARMRSPRPAILLMVGDGPLRATVESLVPEELRP
                     KLRFLGFQRDVQPIILASDVLLLTSVEESFGLVIVEAAACGRPAIATACGGPEEIVRD
                     GETGYLVPCQDPDALGRALTRLASQPELARQMGQRARTWALERFHPQNFSSLMVAALE
                     SLVATQQQRS"
     gene            2549..>3055
                     /locus_tag="PKI05_08965"
     CDS             2549..>3055
                     /locus_tag="PKI05_08965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOA52376.1"
                     /translation="MSRPLVSAIIPVFNGAQYLAKAIDSVLAQTYSPIEVIAIDDGST
                     DESPEILAGYGDKICCIRQVNRGVGAARNRGILAARGEYVAFLDQDDWWYPQKIERQL
                     AALANSDVGFVHTHTVYFDDVTQTFCPPLDPEAKPEEYVGWCFEKLLFGNAICNSSVL
                     VRRELFAQV"
BASE COUNT          590 a          828 c          886 g          751 t
ORIGIN      
        1 gcccagcgaa ttctcgattt ctttcggcga ctcccacaat cccatgtggg ccaggtgata
       61 tacacacagt atgaacaact cctggccaat cgtggcgagg tcgaacgggt catcaggttt
      121 ctgggctgcg atcctggtac atttccttgg gatcggtttg agaatctgcc ggtgcgggga
      181 tcgagctttt atcgtggcaa tcgccaggag cttcactggc aacccgttga aaaaggaaag
      241 gattttcagc ctgttggccg ttggaaacat tggccctggt gggttcgctg gcaattccgt
      301 cggattgccg gacgggaatt cgaggcctta gggtacactt ggtcagcggg gaccgagtca
      361 attcggtagg ccggtgctat ggcagaaact tcaggaccgc cccgtgaatt tccgcggatc
      421 agcgtaatca tccccgtttt taacggcgag cgctttttgg gcgaggcgat taagtcagct
      481 ctttcccaga cgttgccgcc ctatgaaatc ctggtgattg acgacggatc gacggaccga
      541 tcggcccaac ttgcggaatc gtttggaccc ccagttcgcg tgatccggca agagaaccga
      601 ggcgaggctg ccgctcgcaa tcggggaatt gacgctgccc agggagattg gatcgccttc
      661 ctcgattgtg atgacgtctg gaagcccgaa aagctcgcgt tacaagcggc gctcctttcc
      721 ccggcggtta attgcgtcca taccaacttc ttctattttg gagccagcca agggaccgtg
      781 gatgtgagtg atgtcccacc ggccacacgg tattcgctgg gttatgtggc acggcgaaac
      841 ccttttcgca tctcgagcct gctggtacgt cgcgatctgg atattcgctt cccagaatgg
      901 acacaagatg gcgaagacct gatctatttt ttggaattag caagaaagtg cgagattcga
      961 ctggttcggg agtttgtgac gggctatcgc gtccatgcaa agggtcaaag ttcgcggccg
     1021 gacatgttgg ttcgccgctt ccatgccctc cagcagtttc tggagggcgt tgccgatctc
     1081 cccgttgcgg agaaagacct cgttcaacgt gaatacttag ctttgatggc agaacaggca
     1141 accctggcaa aataccgtcg caactggcag gagtattggg cacttcgccg ttttttggca
     1201 tcgtggaagg gcccacttcc cgcggaagcc aaaccggttc tcagggaatt catctttccg
     1261 ccacttgtct atcgagtcaa agattggttc gatgctctca ctcgctgtga tgccgattcg
     1321 ggctcggggt gatcgttaat ggcattacag acgccagcaa gagaagtgat tgctcatgag
     1381 agtcgtcagc ttcgcatttt cattgcggcg cattccggcc agcagggtgg ggcggagttg
     1441 tgtttggatg ttttcctcgc acatcttccg cgtgctcgct ataaagccac ggtcttgttt
     1501 ggttgtgaag gccccatggt gcaggccgcc gcggagaaag gctatccgac ccaagtggtt
     1561 ccctttgttt ggtggttcgg atatgaaccc tgctcctggt attggcggaa tcttctcaag
     1621 tcaccttttc gggtcgcacg cttggcgaga ttgcttcgcc aggagtcctt tgatgtggtc
     1681 tatactaatt ctgccgtcat cttcgaagcg gccttggctg cgcggatagc tcactgtccc
     1741 cacctctggc atgtccatga aatcttacgg cctcaatcgt ggcgaagttg gcttccgatt
     1801 cgcacgatct gtcgcctgat tgatcggtgg tcggatctta tcgtgttcga atcgcaggca
     1861 gccaggaaca tttttgcgag cctgcatcag cccaagacaa aaacagacgc cgtttcgaat
     1921 cccgtgcgtt ttcttacgga tatcgaacca tctcacgatc ccgcagtcat ccgtaatacg
     1981 ttgggactgc cgcaggatgc cttgattgtc gtttggattg ggcaattcat tccccggaag
     2041 aatccccagc ttgccatcga ggggtttgcc cgaatgcgca gtccacggcc tgcaattctt
     2101 ctcatggtag gcgacggtcc cctacgcgct accgttgagt cgctcgtccc tgaggagttg
     2161 aggccaaagc tccggtttct tggtttccag cgggatgttc agccgattat ccttgccagc
     2221 gacgtgctct tactcacctc tgtggaggag tcgtttggac tggtcattgt ggaggcggcg
     2281 gcttgtggaa ggccagccat cgctacggct tgtggtggtc ccgaagagat cgttcgcgat
     2341 ggagagaccg gttacctggt cccttgccag gatccggatg cccttggacg agccctgaca
     2401 agactggctt cccaaccgga attggcgcgc caaatgggcc agcgtgcccg aacctgggcg
     2461 ctcgagcgat tccatcccca gaacttttct tctctgatgg tggccgccct tgagagtctt
     2521 gtcgccacac aacagcaacg gtcgtgatat gagtaggcct cttgtcagcg cgattatccc
     2581 ggttttcaac ggggcccagt atctcgccaa agcgatcgac agtgtgctcg ctcagaccta
     2641 ttctccgatc gaagtgatcg ccatcgacga tggatcgacc gacgaatcgc cggagatcct
     2701 ggcgggttac ggagacaaga tttgctgtat ccgccaggtg aaccgcgggg tgggcgcggc
     2761 gcgcaatcgg ggaatcttgg ccgctcgcgg tgaatatgta gccttcctcg atcaggatga
     2821 ctggtggtat ccccaaaaga ttgagcggca attggccgct ctggcgaact ctgacgttgg
     2881 atttgttcac acccacaccg tgtacttcga cgacgtgaca cagacgttct gcccgcccct
     2941 tgatccggag gccaaaccgg aggagtatgt cggctggtgt tttgagaagc tcctgtttgg
     3001 caatgccatt tgcaactcca gtgtccttgt gcggcgggaa ctcttcgcgc aggta
//
LOCUS       DAOPTR010000434         3050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_25681_length_3050_cov_3.851419, whole genome shotgun sequence.
ACCESSION   DAOPTR010000434 DAOPTR010000000
VERSION     DAOPTR010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25681_length_3050_cov_3.851419"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="PKI05_08970"
     CDS             complement(<1..1179)
                     /locus_tag="PKI05_08970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA52377.1"
                     /translation="MKLPPEGAVWLVRLRWFVALCVFAATFLGYFVFHVLATPIPLIL
                     VGVLMLVYNGILALVVREDSPIRGLRPEDSIFAQIILDFAALTLVLYFSDFSHNPFIL
                     YYIFHIIIAGMYLRGIRPYLVAGGTTLVIGAIIWAEYVGWLPRFPLRFGGQELPPLHG
                     LQALLLFLAMASSLGLATYFTTSIRRYVDRAHAELRQKEKMLGIGQLVAGIAHQIANP
                     LDGVQNCLRRIGEAVKNDARLTEYVQMMAEALERIERTAKRVQAFAQPRGVSLEKTDV
                     NAAVEGTLAMLGTRIGNNVRVEKDLRQVPAVLGDKYTIQEVVFNLCTNALAAMPNGGT
                     LTLRTRTLGTQDDDQMNSVAIEVSDTGVGIPRANLEKIFEPFFTTRAESGGTGLGLGL
                     C"
     gene            1470..>3050
                     /locus_tag="PKI05_08975"
     CDS             1470..>3050
                     /locus_tag="PKI05_08975"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOA52378.1"
                     /translation="MRVSRLSRLWVCWPLLGLLVLAVGCPQSSVDSSAFRVKTASQEP
                     STSNSNTSEGAAAPAESASTGPVTQQTVTTPPPTPEQNQQVPSPVATPNPPVSENASS
                     QPSPTPPSASHGTDQAAVNTESPPPASPPSPPATDLLALVNPATHCLMGQWTREGKGF
                     RSPAQAYTTLVFPFDPPGQFRLLIVARRIEGYESLNVTLPVGGSQAMVVLDGFGRRIS
                     GLSMVSGRTADANETRFFGFSFLPNMPVPIQFFCSPNRIAVQAGSRWIIDWRGSPGVL
                     SLDSRFWKHIPPNRIAISVYSPNTVFVIERAELTPLPEGLGLPEETLVPGRPGSSLAG
                     GPGSRPPFGPPWGRFDGPPESPAESPSVQESASPPASQTASEPPPSNVPVMHFHEEPP
                     EAVTQWKDSVGLIEYPLGSGTGFVAKENLIATNAHVIEGAFAEDLEISFTGAQEGRYR
                     VQKILFEDPARDLAILYVTCPQKPVTLAQNSELRTGDKVAIISNPSLGKTDIVVRNAI
                     VAGQVAARVHTKGYDFYQV"
BASE COUNT          698 a          892 c          804 g          656 t
ORIGIN      
        1 gcaaaggcct aatcccagac cagtcccccc cgactctgcc cgcgtcgtga agaacggctc
       61 gaagatcttt tccaaattgg cccgcggaat ccccacaccc gtatcactga cctcgatggc
      121 cacggagttc atctggtcgt catcttgggt tccaagggtc cgcgtgcgaa gggtcaacgt
      181 tcccccattt ggcatggccg ccaaggcgtt cgtgcataga ttgaagacaa cctcctggat
      241 ggtgtattta tctcccaaaa ccgccgggac ctggcgcaaa tctttctcta cacgaacatt
      301 gttaccgatt cgcgtcccca gcatagccag ggtcccttcc acggccgcgt taacgtccgt
      361 tttctccaaa gagacacccc ggggctgggc gaaggcttga acccgctttg ccgtccgttc
      421 gatcctttcc agggcctccg ccatcatctg gacgtattct gtcaaccgtg catcgttctt
      481 gacggcttcc ccaatacgac gcaagcagtt ttgaacaccg tcaagcgggt ttgctatctg
      541 atgagcgatc ccggccacaa gctgcccaat gcccaacatc ttctccttct gccgcaactc
      601 agcatgggca cgatccacat accgacggat cgatgtggta aagtatgttg ccaaacccaa
      661 actgctggcc atggctaaga acaacaaaag ggcctgcaaa ccgtgcagtg ggggcaattc
      721 ctgaccacca aatcgaagag gaaatcgcgg cagccagccc acgtattccg cccagatgat
      781 ggccccgatg accaaggttg ttcccccagc aacgaggtag gggcgaatcc ctcttaagta
      841 catgccggcg ataatgatat ggaaaatgta gtaaagaatg aacggattat gcgaaaaatc
      901 ggagaaataa aggacaagcg ttaacgcagc aaaatccaaa atgatctgag cgaaaatgct
      961 gtcctcaggc cgtaggcctc gaatagggct gtcttctcgc acgacaaggg ccagaatccc
     1021 attgtaaacc agcatcagaa cgcccacgag aatcaggggt atgggcgtgg ccaacacgtg
     1081 aaaaacaaaa taccccaaga aggttgccgc aaagacgcac aatgcgacga accaccgcag
     1141 tcgtacaagc caaacggcac cctcgggtgg aagtttcatc gttgagaatt cctattgata
     1201 ctttttttca ctctaggcag ggaaggacgt tccggcaagg ctcggcaatc cggcaaaatt
     1261 gccttcagcc acaaaggttg ccgcagggca cggagcgcgc gtccgccggt ccaaggctac
     1321 cccttctcgc gcaaattgcg gacactggtg ttttggtcac ttcgcgggct tttccccgta
     1381 gcgcgttgca tttgctttgg cagaattgta gaattcgatc atcgaatacc ctaatccaat
     1441 caagaggtct tccagggcag gagttgacca tgagagtcag cagattgtct cgtctctggg
     1501 tttgttggcc cctgttaggg cttcttgtgt tggcagtcgg atgtccccaa tcgagtgttg
     1561 attcgtcggc gtttcgggta aagaccgctt ctcaggaacc atcaacgtct aattccaaca
     1621 cttccgaggg ggcagcggct ccggcagaat ctgcgagcac tggtcccgtg acacagcaga
     1681 ctgtgacaac ccccccgcca actcccgaac aaaaccaaca ggttcccagt cccgtagcca
     1741 caccgaatcc accggtttca gagaacgcct ccagccaacc cagtccgaca ccgccttcag
     1801 caagccatgg gaccgatcaa gcggccgtaa acacggaatc tccgccaccg gcatcgcccc
     1861 ctagccctcc agccacagat ttgttggcac ttgtcaatcc ggcaacacac tgtttgatgg
     1921 gtcagtggac gcgagagggc aaagggtttc gttcgccggc ccaggcttac acgacgttgg
     1981 tttttccttt tgacccgcca ggtcagttcc gtctgttaat tgtggcacgg cggatcgagg
     2041 gctacgaatc gctcaatgtc acgcttcctg taggtgggtc acaggcgatg gtcgtcctgg
     2101 acggtttcgg gagaaggatc agcggcctgt cgatggtgtc gggaagaaca gctgatgcca
     2161 atgagacgcg gttctttggc ttttccttcc tgcccaatat gccggtgccc attcagttct
     2221 tctgttctcc gaaccgcatt gctgtgcaag ccggaagtcg ttggatcatc gactggcggg
     2281 ggagtccggg ggtcttaagt ttggatagtc gcttttggaa acacattccc cccaaccgta
     2341 ttgccattag tgtttatagc ccgaatacag tgttcgtgat cgagcgtgct gaacttacgc
     2401 cgcttccaga gggcttaggg ctacccgagg aaacgttggt gccggggcga cctggttcgt
     2461 cacttgccgg aggccccggc tcccgtccac cgtttggccc tccatggggc cgtttcgatg
     2521 ggcctccaga atcccctgca gaatcccctt cagtccaaga gtcagcctcc ccaccagcgt
     2581 cgcaaacggc cagcgaaccg ccaccgtcca acgtcccagt gatgcatttc cacgaagaac
     2641 cgccagaagc ggtgacccag tggaaggact ctgtcggctt aattgagtat ccgctcgggt
     2701 cgggtacggg attcgtcgct aaagagaatc tgattgcgac aaacgcccac gtcatcgaag
     2761 gagcgtttgc cgaagaccta gaaatttcat tcactggtgc gcaggaagga cgttatcgcg
     2821 tgcagaaaat cctttttgaa gacccggcga gggacttggc gatcctttat gtgacttgtc
     2881 cgcaaaagcc ggtcaccttg gcacaaaaca gcgagctgcg aacgggcgac aaagttgcaa
     2941 ttattagcaa tccttccttg ggcaagacgg acatcgttgt tcgtaatgct atcgtcgctg
     3001 gccaggtcgc cgcgcgagtt cataccaagg gctacgactt ttaccaggtc
//
LOCUS       DAOPTR010000435         3047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_25711_length_3047_cov_3.931150, whole genome shotgun sequence.
ACCESSION   DAOPTR010000435 DAOPTR010000000
VERSION     DAOPTR010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25711_length_3047_cov_3.931150"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(238..1956)
                     /locus_tag="PKI05_08980"
     CDS             complement(238..1956)
                     /locus_tag="PKI05_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOA52379.1"
                     /translation="MAVKRRVDYTDLLIRKGIISADQLEEAKQLAAQSGMKLPDAIIR
                     LGYASGEEVMQVMAEQHGLEYIDLNRISIPPNVVELVPESVARENVVIPIAEDDGQLR
                     VAVCDPDDFATFDKLRFILNRRIEICLAPREMILEAINRYYGQTSGESQDSVLSEVTD
                     TAIDFTQVETDMGSIQDADETSAPVVRLVHLIISEAVQLRASDIHIEPFEDRVRIRYR
                     IDGYLIERDNAPRRLLGAILSRIKILARMDIAERRRPQDGRIKMNIGEKQLDLRVSVL
                     PSTWGQAVVMRILDKDNIKIGLRQLGFSEEDYQKFRQLIKRPNGIILVTGPTGSGKTT
                     TLYAALNELNTPDRKIITAEDPVEYYLPGINQVEIRHEIGLDFARVIRAMLRQAPNVI
                     LVGEMRDLETAQMGIQASLTGHLVFSTLHTNDAPSSITRLIDMGVPAYLVASSVVAIM
                     AQRLVRVVCEQCKQPYQPSDAVLEMAGITPEMAATATFYRGKGCSRCNGTGYRGRMGI
                     FELMIMSPKIREMTFRQAPVADIRRVAIQEGMRTLYMDGLQKVLRGRTTLEELLSVAR
                     PEEELR"
     gene            2274..>3047
                     /locus_tag="PKI05_08985"
     CDS             2274..>3047
                     /locus_tag="PKI05_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52380.1"
                     /translation="MAYLLGLLLALHLTSLVVMGDTANTTVSFDEKKLLPPWPDESPL
                     LEGPELTLPEIETPTTTSWVWISSQEMEPGELSGPTFEGFLGNGGPADDPSAEPTGDS
                     LLIPEDQVFAPDAVSVRGATVATSGGVRCCSEDPSGCRPRRGIVRGLFADLQNAGRWQ
                     RWRSEPGSAGVFLGYGWGTELVNNWLEERDGVWGGLRLGWDCSEQYGVETRLSFGTLD
                     LWDHPDAVAAGRGLGLNGARDRWLRAVEWDAVLLYYPTEN"
BASE COUNT          607 a          880 c          859 g          701 t
ORIGIN      
        1 atgtgtaagt cgcttgcttt tcgagcaacg acggtattga gcagcttgtc aatctgtaat
       61 tgcgacatac tgtccgtcca aaccccgatg ctttgaagac cagaaacgct aactatttac
      121 cccatctccc gcataattat cgcgattgat gtcctcacca aatgggcctt accggctaac
      181 agaaccccag tggcatccca atagcaacaa ccgggaaagg cgtcgccgtt ggaatactca
      241 gcggagctcc tcttccggtc gggcaactga caacaactct tcgagagttg tccttccccg
      301 caacaccttt tgcagaccgt ccatgtagag ggtcctcata ccctcctgga ttgctacccg
      361 ccggatgtcg gcgacgggtg cctgacggaa ggtcatttcc cgaatctttg gcgacatgat
      421 catcaattca aaaatgccca tgcgtccccg gtagcccgtc ccgttgcacc ggctgcatcc
      481 tttgccacga tagaacgttg ccgtggcagc catctcgggg gtaatccctg ccatttccaa
      541 aacggcgtcg cttggctggt aaggctgctt gcactgttcg cacaccaccc gaacgaggcg
      601 ctgcgccatg atcgccacga cgctgctcgc cacgaggtag gcggggacgc ccatatcgat
      661 caggcgtgta atagaactgg gcgcatcgtt cgtatgtagc gtactaaaaa cgaggtgtcc
      721 cgtcaaagag gcttggattc ccatctgcgc agtttccagg tcacgcatct cgcctaccag
      781 gatcacgttc ggggcttgtc gcaacatcgc ccgaatgacc cgggcgaagt ccagcccgat
      841 ctcgtgccgg atctcaacct ggttgatccc ggggaggtag tactccacgg gatcctctgc
      901 ggtgatgatc ttgcgatccg gtgtgtttag ctcgttcaag gccgcataga gggttgtcgt
      961 cttccccgaa ccggtgggtc ccgtcaccag gatgatgccg ttcgggcgct taataagctg
     1021 gcgaaacttt tggtaatctt cttcagagaa gcccaactgc cgcagaccga ttttgatatt
     1081 gtctttgtcc aaaatccgca tcacgacggc ctgcccccac gtgctgggga ggacacttac
     1141 ccggaggtca agctgctttt ctccgatatt catcttgatc cgaccgtctt gcggccggcg
     1201 acgctcggcg atatccattc gggccaaaat cttgatacgc gatagaatgg cccccagcag
     1261 ccggcggggg gcattatccc gctcaatgag gtatccgtcg atccgatatc ggatgcggac
     1321 acggtcttcg aacggctcga tatgaatgtc cgacgcccgc aattggacgg cttccgagat
     1381 gatgagatgg accagccgga ccaccggggc gctggtttca tccgcatcct ggatgctgcc
     1441 catgtcggtt tcgacttggg tgaagtcgat cgccgtgtcg gtaacttcgc tcaacacgga
     1501 gtcttggctt tcgccggagg tctggccgta gtaacggttg atcgcctcca agatcatttc
     1561 gcgaggtgcc aggcagattt cgatgcggcg gttcaggata aaccggagtt tgtcaaaagt
     1621 ggcgaagtca tcgggatcac acactgccac ccgcaattgg ccatcatctt cggcgattgg
     1681 gatgaccacg ttttcccggg caaccgactc gggtaccaac tccaccacgt tcggaggaat
     1741 agagatacgg ttgagatcga tatactccag gccatgctgt tccgccatga cctgcatcac
     1801 ttcctcccct gaggcgtacc ccagtcggat aatcgcatca gggagtttca tcccagactg
     1861 ggcggccaac tgcttggctt cctccaattg gtcggcgcta atgatccctt tgcggatgag
     1921 gagatccgtg taatcgacgc ggcgtttgac ggccatggaa tccgcctccc atggttggac
     1981 aaggatttgt tttgctcacc tataacatat ccccaattcc acagtacccc tgatcgcgtc
     2041 acctgtcaac aacgcatgtt gacagaatgc aatattttcc cgataatccc ccggcttttg
     2101 actgccgttc cgtttcccgt tggacgctca gcggaatttc ggttgtgcac caagtgccat
     2161 ccaatcatct cccggccgcg cgtttgaagc gctgggcacg gaagcgatta tgtgttccgt
     2221 tcccaagtgc gatcgtgctg tttcttcgca ggtcgagcag acgatggccg tggatggctt
     2281 atttgcttgg gctactgctc gcactccatc ttaccagtct tgtggtgatg ggagataccg
     2341 ccaacaccac ggtttcattc gatgagaaga aacttctccc gccctggcct gacgaatctc
     2401 ccttgttaga gggacccgag ctgactttgc cagaaatcga gacaccgaca acaacaagtt
     2461 gggtctggat ttctagccag gagatggaac cgggcgaact ttcgggcccg accttcgagg
     2521 gctttcttgg caacggcgga ccggccgatg atccttccgc cgaacccaca ggggacagcc
     2581 tcctgattcc tgaggatcaa gtgttcgccc cggatgccgt atcggttcgt ggtgcgaccg
     2641 tcgcgacctc tgggggcgtt aggtgttgct cggaagatcc ctctggatgc cgacctcgtc
     2701 gggggattgt gcgcggtctg ttcgcagacc tgcaaaacgc cggacgctgg caacgctggc
     2761 gcagtgaacc gggcagcgcc ggtgtgtttc tgggctacgg ttgggggact gaactggtca
     2821 acaactggct ggaggagcga gacggcgttt ggggcggact gcgacttggt tgggattgtt
     2881 ccgagcagta tggggtcgag acacgcctct cctttggaac tctggacctt tgggatcatc
     2941 ccgatgcggt ggccgcaggg agagggcttg gacttaacgg cgctcgggac cgctggcttc
     3001 gggcggtgga gtgggacgca gtattacttt actaccccac agagaat
//
LOCUS       DAOPTR010000436         3043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_25746_length_3043_cov_3.369143, whole genome shotgun sequence.
ACCESSION   DAOPTR010000436 DAOPTR010000000
VERSION     DAOPTR010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25746_length_3043_cov_3.369143"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1846)
                     /locus_tag="PKI05_08990"
     CDS             complement(80..1846)
                     /locus_tag="PKI05_08990"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HOA52381.1"
                     /translation="MVDAVAGSAPRSDSVEPPTTEPLPWQERLTRGGVKPAVNDVLLL
                     SYPDDPDTLNPITSSDTVSEAFQSRVYETLAEPRFENPDEWDPVLAESWDFDEEKLEF
                     TIRLRKGVKWHPMKLPNGQPLPDAELTARDVIFSFDCVMNPNVEAAHIRSYYEDPSAT
                     DEEGRRMIEYKAVDKYTVKVRWKKPYFLAKEFTLNGFAIIPRHVYSVDAEGKPISFDF
                     SSKEFADGFNNHWANKMMCGTGPMIFKEWVSQQRLVLVRNPDYWGEPYYFSGVTYRCI
                     SNSNTNMQMLLQNELDFSGIADKDLFLQAKEHPNVKAGKVCLVDYPYPGYRYIGYNLD
                     KPVFKDREFRWALAYAIPVDTIIEKVFRGLAGRTTGPFLPGSSSYDETLPPIPYDLNK
                     AREMLEKAGWVDTDQDGVRDKVIDGQKVPARFELMIYTDAPSYRQIAEIVKEECRKIG
                     IDVQIAPTKWNLMLQKLRKREYEAAMLGWAMSWKQDPFQLWHSSQADAPDSSNHVAYR
                     NPEVDRLIEELRVTMNEEKQHELYKKIHRLIYEDQPYTFLFSDRATGGYDARIQNVKF
                     YKIRPCYDGREWYSNRPRAVGP"
     gene            2364..>3043
                     /locus_tag="PKI05_08995"
     CDS             2364..>3043
                     /locus_tag="PKI05_08995"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOA52382.1"
                     /translation="MSHPSSGEGIIQAEVLAVEPAHRKSVFWRVLKVLIGLLVIVTLF
                     VSLLLNMFFIGTLGLDGMGSKKVREKYFSHSRFARDKVVIMPLEGLILDGDGFMKRQI
                     DQVIEDETVKAVVLRVNSPGGTVSGSHAIYHYLKKMCEKRKIPLVVSMGAIAASGGYY
                     VSMAVGDTPKSIYAEPTTWTGSIGVIIPHYDAAELAQKIGIKEDSVASHPLKGMGSML
                     RPMTEEERA"
BASE COUNT          707 a          793 c          823 g          720 t
ORIGIN      
        1 cgaaaattgg accatcccgg ttgtcttcag aaacccacgg cggacatagc aaaacatgac
       61 ccttcctgta cgggcacact cacggcccaa cagcccgcgg ccggttggag taccattcgc
      121 gaccgtcgta gcagggtcgg attttgtaga acttcacatt ttggatgcgt gcatcgtatc
      181 cgccggttgc ccgatctgag aacaaaaacg tatagggctg atcttcataa atcagccgat
      241 gaatcttctt atacaattcg tgctgttttt cctcgttcat cgtgacgcgc agctcttcga
      301 tcaaacgatc aacctcgggg ttgcgatagg ccacatgatt tgaactgtcg ggcgcatccg
      361 cctggctgga atgccacagt tggaagggat cctgtttcca cgacatggcc cacccgagca
      421 tggcggcctc atactcccgc ttgcggagtt tctggagcat caggttccat ttcgtggggg
      481 caatctgaac atcaatccca atcttgcggc actcttcctt cacgatctcc gcgatctggc
      541 gatagctggg agcatcggta taaatcatga gctcgaaacg ggcagggacc ttttggccat
      601 caatcacctt gtcccgcaca ccgtcttgat cggtgtcaac ccaaccggcc ttttccagca
      661 tctcccgggc cttgttcaaa tcgtacggga tgggcgggag ggtctcatcg taggagctac
      721 tccccggaag gaatggtccc gttgtccgac cggccaatcc ccgaaacacc ttctcgataa
      781 tggtatcaac ggggatggca taggccaaag cccagcgaaa ctcccggtcc ttaaacacgg
      841 gtttgtcaag gttgtagcca atgtaccgat aaccgggata tggatagtca accagacaaa
      901 ccttgccagc cttcacgttg ggatgttcct tggcttggag gaagaggtcc ttgtcggcga
      961 tgcccgaaaa gtcaagttca ttctgaagaa gcatctgcat gttggtgttg gagttggaaa
     1021 tgcaccggta tgtcactccg ctaaagtagt acggctcacc ccagtaatcc ggatttcgca
     1081 cgagcactag tcgttgttgc gacacccatt ccttgaagat catgggaccg gtgccacaca
     1141 tcatcttgtt ggcccagtga ttgttgaagc cgtcggcaaa ctctttggac gagaaatcga
     1201 acgagatagg ctttccctcg gcatccaccg agtacacgtg ccgagggatg atggcgaatc
     1261 cgttcagggt aaactctttg gccagaaaat acggcttttt ccagcggact ttgacggtgt
     1321 atttgtccac ggccttgtat tcaatcatcc ggcggccctc ttcgtccgta gccgagggat
     1381 cctcgtaata gctgcggata tgggcagctt caacgttggg attcataaca cagtcaaagc
     1441 tgaagatcac atcgcgggcc gtcagctcgg catcggggag cggttgacca ttggggagct
     1501 tcatcggatg ccatttgacg ccttttcgca aacggatcgt gaactccagt ttctcctcat
     1561 caaaatccca actttccgcc aaaacagggt cccactcatc gggattctca aaccttggct
     1621 cggcaagggt ctcgtacacc cgcgattgaa aggcctcgga taccgtgtcc gatgaggtaa
     1681 ttgggttgag ggtatccgga tcgtcgggat aactcaaaag gagcacgtca ttaacggcgg
     1741 gtttgactcc ccctcgagtc aatcgttctt gccatggaag aggttcggta gtcggtggtt
     1801 caaccgaatc gctgcggggt gcggaaccgg cgaccgcatc aaccatgctt gtgtccgatt
     1861 tcgtgcaacc cgaaaaagcg gctaagatca ctaaaatcgg cgcgactttc actaatgaca
     1921 gcctctccat atattactcc ctttccacgc agccgctgga tcacgggatg gagttaactg
     1981 aactcgacaa aatgagtcct gtaagctccg tcagcgacgg gcgaccattc aaagtcatta
     2041 ccacagcgaa cgggaacggt atcaccctcc cgttgggatt cgattcctgg tatctgaaac
     2101 aaggtgcagc cagattaaaa ctccaccgtt tgtggcatgt taagatattg ggatgatgcg
     2161 gtcaataacg accggcgacc gttcggcgag ttggtgagtt ggttaatcct gcaaagcaac
     2221 gtaaaataac gcaggttctg attcaacggt gggaaagcgg gtatttttca catcacggcg
     2281 gcacggcggt agccgtacgt gtgtggcgtc atcggcagtt ctccgaagtg tcggaccttt
     2341 ttacttaaat gttggagcag catatgagtc acccatcttc tggagaaggc attatccaag
     2401 cggaagtgtt ggccgttgag ccggctcacc ggaaatctgt cttctggcgc gttctcaagg
     2461 tcttgatcgg cttgctcgtg atcgtgaccc tatttgtgtc cctgttgctc aacatgttct
     2521 ttatagggac gttagggctg gatggcatgg gcagtaagaa agttcgggag aaatattttt
     2581 cccactcccg atttgcccgg gataaggtcg ttatcatgcc gctggaaggc ttgatcctgg
     2641 atggggacgg ctttatgaag cggcaaattg accaggtgat cgaagacgaa acggtcaagg
     2701 ccgttgtcct tcgggtgaac tccccggggg gaaccgtgtc gggatcgcat gccatttatc
     2761 actacctcaa aaagatgtgt gaaaaacgaa aaatcccctt ggtggtcagt atgggggcaa
     2821 tcgctgccag tgggggctac tatgtgtcga tggccgtcgg cgatacgcca aaatccattt
     2881 atgccgagcc cacaacgtgg acagggtcca tcggtgtgat catcccccat tatgacgcgg
     2941 cagaattggc acagaaaatc ggtatcaaag aagattctgt ggccagccac cccttaaaag
     3001 ggatgggcag catgctacgt ccgatgacag aagaggagcg agc
//
LOCUS       DAOPTR010000437         3041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_25775_length_3041_cov_3.790355, whole genome shotgun sequence.
ACCESSION   DAOPTR010000437 DAOPTR010000000
VERSION     DAOPTR010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25775_length_3041_cov_3.790355"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2261)
                     /locus_tag="PKI05_09000"
     CDS             complement(<1..2261)
                     /locus_tag="PKI05_09000"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOA52383.1"
                     /translation="MKAFHLPCQVNLIRVGTLGSVLFLLATLSTGDSVYGNDNVPTGP
                     VVDVQMTEEMLVADFLRQAELRESKAHRGSAQHPSEDARGGCDGIKNGQWGFHTNREP
                     QPWWQVDLGREYPITKVMIFNRCDTVAHRASRLHVLVSGDGQSWTKVYEHDGTVFYGQ
                     TDGKPLVVDLPGVAARFVKIQLPDTEYLHLDEVEVYSSEDQEQANIALGKRADQSSLS
                     QWSVYHPEVPQEGPIFPLEKVLERGLALAEELLEMPSLVEPARGIVEREKAYLKELAS
                     RVARSKLSSEERRQLYCDACLAVRRMSWQNPLLDFRDLVFVKRKPPIFHHMSDQHYGW
                     WCQGDGGLFILEDWKSEHPRLRCLTASSPSGSVLRPDISYDGRRILFAYARYYPGLHQ
                     LANKLDKNNVPEDAFFHIYEINVDGTGLRRLTYGKYDDMDARYLPDGRIVFLSTRRGR
                     QTQLLPRPVNYPGESANPDCYVRCGGGPERPVAIYTLHVMDGNGENIRPISPFESFEW
                     HPSVDFSGRILYARWDYVDRHNQPFMGLWATLPDGTGVQAIYGNFTWNPLSIFEARAV
                     PGSHKIVFTASAHHSITGGSLVLLDPRRGFDDEAPLTRLTPEVCFPESEGWPLHYYAN
                     PYPLSETFMLVSWSDQPLADHAGRMSDVGSGIYLYDTFGNLNLIYRDPTIGCETPLPL
                     KPRAKPVVASAETTETSAEEGYFVVSDVETGMTGIARGTVKRLRLVGIPVKTHPTMNY
                     PELGLTREDPGKFV"
     gene            2617..>3041
                     /locus_tag="PKI05_09005"
     CDS             2617..>3041
                     /locus_tag="PKI05_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52384.1"
                     /translation="MVMYRVVVIETPEDWNPSSLDDVPPQPPEPKVQLGEYPELFPAV
                     QAAIEHNRQSQNQRDRRWAVVCEVGQGGKTWRGLRICTPLRYKIASIWWPAGWEPLSP
                     FDVPLCVCRTQGTLQEDVLTYDQALATMKALNLQAMDRA"
BASE COUNT          703 a          860 c          822 g          656 t
ORIGIN      
        1 acaaacttgc ccgggtcctc gcgggttaac cctaactctg ggtaattcat cgtcggatgc
       61 gtcttgactg ggatgccgac aagccgcaga cgctttactg tccctcgcgc gatccccgtc
      121 atgcctgttt ccacatcact aaccacgaaa tatccctctt cagcacttgt ctctgtggtc
      181 tcggcagacg ccacgaccgg ttttgctcgt ggttttaatg gcaggggagt ttcacatcct
      241 atggttggat cccgataaat aaggttgaga ttcccaaagg tgtcatacag gtaaatacca
      301 cttccgacat cggacattcg acctgcgtga tcggcaaggg gctggtcact ccaagagacc
      361 agcatgaagg tttcgctcag gggatacgga ttggcgtaat aatggagggg ccagccttca
      421 ctttcgggaa aacacacttc tggggtcaag cgggtcaagg gtgcctcgtc gtcaaaaccc
      481 cgacgggggt ctaaaaggac cagacttccc cccgtgatgg aatgatgggc tgaggccgta
      541 aagacgatct tgtgcgaacc cggaacggcc cgggcctcga agatactcaa gggattccaa
      601 gtaaagtttc cataaattgc ttgcacaccg gttccatcag gcaaggttgc ccaaagcccc
      661 atgaatggct gattgtgacg atcgacatag tcccatctgg catagaggat tcgcccggaa
      721 aaatcaacgc tcggatgcca ttcaaagctt tcaaacggcg aaatcggacg gatgttttcg
      781 ccgtttccat ccatgacgtg aagtgtgtaa atggcgacgg gccgttcggg accacctcca
      841 caacgaacat aacaatccgg gttcgcactt tcccccggat agttcaccgg ccgaggcaga
      901 agctgggtct ggcgtccgcg tcgggtggaa aggaagacga tccgcccatc gggcagataa
      961 cgagcatcca tgtcatcata tttgccgtaa gtcagccgac gcaaacctgt tccatctacg
     1021 ttgatttcgt agatgtgaaa gaaggcatct tcggggacgt tgtttttgtc caatttgttg
     1081 gccaattgat gcagaccagg ataataccgc gcgtaggcga agagaatacg ccgtccatcg
     1141 taagagatgt caggacgcag cacggatccc gaaggtgacg aagccgtgag acaacgcagg
     1201 cgagggtgtt cgcttttcca gtcctcaagg atgaacaggc cgccatcgcc ttggcaccac
     1261 cacccgtaat gctgatccga catgtgatgg aagataggtg gttttcgctt gacgaacaca
     1321 agatccctga aatcgaggag cggattttgc caactcattc tccgaacggc caagcaggca
     1381 tcgcagtaga gctgccgcct ttcctccgaa gaaagctttg aacgggcaac acgcgatgcg
     1441 agctctttca ggtaagcctt ttcacgctcg acgatccctc gggcgggttc aaccaaggag
     1501 ggcatttcca ggagttcctc ggccaaggcc agacctcgct cgagaacctt ttccaacgga
     1561 aaaatcggcc cttcctgtgg cacctccgga tgatacaccg accactgact gagactgctt
     1621 tggtcagccc gtttgcccag ggctatgttt gcttgttctt gatcctccga ggaataaacc
     1681 tctacctcat ccaagtgcaa atactcagta tcggggagtt ggattttgac aaatcgcgcg
     1741 gccactcccg gaagatcgac aaccaagggt tttccgtcgg tttggccgta aaacaccgtc
     1801 ccgtcatgct catagacctt cgtccaggat tgaccgtcgc ccgagacgag cacatggaga
     1861 cggcttgccc ggtgggctac ggtgtcacag cgattgaaga tcatgacctt ggtgatgggg
     1921 tactccctgc caaggtcaac ctgccaccac ggttggggct cgcgattggt gtggaagccc
     1981 cactggccat tcttgatccc gtcacaacca ccccgggcgt cttctgaggg atgttgcgcc
     2041 gagccgcgat gcgcctttga ttcgcgcaat tccgcctgac gaagaaaatc ggcgaccagc
     2101 atctcctcgg tcatctggac atcgacaacg gggcccgtcg gcacgttatc gttgccgtac
     2161 acggaatccc cagtagaaag agtcgccaga aggaaaagca cgctcccgag ggtacctacg
     2221 cggatgaggt tgacttgaca gggcaaatgg aacgctttca ttttggaccc tccgagcctt
     2281 ccagcgaatg cctggcctac aattaattgg aagctcgcag cctatatgtc cagaatcggc
     2341 cgtaccccaa cggatccggc attactacct ccggagtgtg caacctccgc ttaaacatcc
     2401 gtcccacacc tgcagcgata aacaccaaga ctgcttgaca tattacctga caggagcgaa
     2461 aagcacaact tggcaggtgc ctttccccgg ggagtcagtc ctttgttcgg ggactttatc
     2521 aatgcctgga aagcctatac catgaacgaa gcgaccaagc gtgatgccgc ctgaacgctg
     2581 cgacgtattg ttcaaatctt tcccgaggtt atctcaatgg tcatgtatcg cgtggtggtc
     2641 atagaaactc cggaagattg gaatccttct agccttgatg atgtgccacc tcagccaccg
     2701 gagccgaaag tccagctcgg agaatacccg gagctattcc cggcggttca ggcggcgatt
     2761 gagcacaatc ggcaatccca aaatcagcgc gaccgccgtt gggccgtggt ctgcgaggtg
     2821 ggacaaggcg ggaaaacgtg gcgcggcttg cggatttgta cgccgctgcg atacaagatc
     2881 gcctccatct ggtggccggc cgggtgggaa cccctgtcgc cttttgacgt ccccctttgc
     2941 gtttgccgga cccaaggtac cctgcaagag gatgtcctta catacgatca ggccttggcc
     3001 acaatgaagg cccttaacct tcaggccatg gaccgtgcta g
//
LOCUS       DAOPTR010000438         3033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_25872_length_3033_cov_4.023842, whole genome shotgun sequence.
ACCESSION   DAOPTR010000438 DAOPTR010000000
VERSION     DAOPTR010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25872_length_3033_cov_4.023842"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..177
                     /locus_tag="PKI05_09010"
     CDS             <1..177
                     /locus_tag="PKI05_09010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOA52385.1"
                     /translation="LVRRCILLGSRPGDLVLDPFSGSGTTGVVAAELRRQAVLIELNP
                     GYARQSRNRITASA"
     gene            863..2926
                     /gene="tkt"
                     /locus_tag="PKI05_09015"
     CDS             863..2926
                     /gene="tkt"
                     /locus_tag="PKI05_09015"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOA52386.1"
                     /translation="MEQSDRSIEQLAINTIRTLSMDAVEAAKSGHPGTPMALAPVGYV
                     IFNDFLRFDPSRPLWINRDRFVLSCGHASMLIYSLLHLAQVEQVDEHGRPTGELAVPL
                     EHIRQFRQLLSRCPGHPEYRHTSGVEVTTGPLGQGVATSVGMAIAGKWLAARYNRPGY
                     DLFDYGIYVLCSDGDLMEGVSSEAASLAGHLKLGNLCWIYDDNKITIEGHTNLAFSED
                     VAGRFRAYGWRVLQVDDVNDLPALRAAIKEFNPAGAQPTLIIVKSIIAYGAPNKQNTH
                     EAHGAPLGEEEIRLAKQFYGWPVEEKFRVPAEVVEHFRQGIGTRGRRLREQWEKKFAE
                     YEKQYPQEADEIRRILNGELPDGWDSEIPTFATDAKGLATRASSGKVLNAVAKRIPWM
                     IGGSADLAPSNNTLLTFPDAGHFSAENYAGRNLHFGIREHAMGAVANGLALCGLRPYA
                     GTFFVFSDYMRPAIRLAALMELPILYIFTHDSIGVGEDGPTHQPVEHLAALRAIPGLI
                     VVRPADANEVAEAYRLALQVRRNPVALVLTRQNLSTIDRTQYAAASGLRCGAYILADA
                     EGGKPDVILIGTGSEVSVCLAARELLSAQGIAARVVSMPSWELFENQPEEYRHSVLPP
                     EVTTRVAVEAGVGLGWERYVGPHGAFVGMRSFGASAPAGVLMKYFGITAENVANVAKA
                     LIKQD"
BASE COUNT          609 a          794 c          950 g          680 t
ORIGIN      
        1 ctcgtccgtc gatgtattct tttgggctct cggcctggag acttagtgct cgatcctttt
       61 tcaggctcag gaacgactgg ggtggttgcc gcagagttgc ggcggcaagc cgttctgatc
      121 gaacttaatc ccggttacgc ccggcaatcg cgaaacagga tcaccgcctc ggcgtagccc
      181 tgcggtggga gggacgcggt cttccgcgtc cgccattttt cttgagcatc gagtacaaat
      241 gctccaggtg atgatcgaag gcaatcgtct tcgggacatt agtgatctcc ccctcgatct
      301 gatcgtttag gctctccggt tgcacccctt tttgagttct tcctgagagt atccctgctg
      361 ggtcagcttt ctctgtcaaa agggtcagaa atcggtgctt ttgccgccgg gcccagaagg
      421 gtgtgaacga tcagaatccg cgtcaagctt ttccgaactt cacgagacga cgttcggtgc
      481 ccagacatgg cggacgtcgc cgggtctgcc attctacgcc agaaaagggg gaacgatttc
      541 gcggcgacgg gggtcggtag gccttggaga agttatggcg tgggattcgc gcgtcgaaaa
      601 gcataggttt cactaggaga acttggcgcc taggaggtaa ccgcgaaggc cctttcaaat
      661 tacgcatctc ggctgtgcca ccacgttgac ggtcactcgt gggcgaccta aattaaacgt
      721 tcacgtattt ggaagatgaa gccgccaatg aagagaaaac tgatcagttc accggtgttt
      781 tgtccaaaac ggggaagttg gccattttga tttctcgttg tgtcaatcag caagtcggtt
      841 agtacacaag ggagttactg tggtggaaca gtctgatcgt tcgattgagc aactggcgat
      901 taacacgatt cgcacgcttt ccatggatgc cgtggaggcg gcgaaaagcg gtcacccggg
      961 gacgccgatg gccttggcgc cggtggggta tgtgattttc aacgactttt tgcgatttga
     1021 tccgtcgcgt cccttatgga tcaatcggga ccgctttgtc ctctcgtgtg gtcacgcgtc
     1081 gatgttgatt tactcgctgc tgcacctcgc ccaggtggaa caggtcgatg agcatggtcg
     1141 gccaacgggg gaactggccg tgcctttgga acacatccgg caattccggc aactgctcag
     1201 tcgttgtccg ggtcacccgg agtaccggca tacctccggg gtggaggtga caaccggccc
     1261 actcggccaa ggagtggcga caagcgtggg catggcgatt gcgggaaagt ggctggcagc
     1321 tcggtataac cgtccgggtt acgatctgtt tgattacggc atttacgtcc tttgcagcga
     1381 tggtgacttg atggaaggcg tttccagcga ggccgcctcg cttgccgggc atttgaaact
     1441 tggtaatctt tgctggattt acgacgacaa caagatcacc attgaaggcc acacaaattt
     1501 ggccttcagc gaagacgtgg cagggcggtt tcgtgcctac ggctggcgag tgctccaggt
     1561 tgacgatgtc aatgacctac cggcgctacg tgcggcaatc aaagagttca atcctgccgg
     1621 tgcgcagcca acgctcatta ttgtcaagag catcattgcc tacggggccc ccaataagca
     1681 aaacacgcac gaggcccatg gggcgcccct tggtgaggaa gaaatccggc tggccaagca
     1741 gttttacggt tggccggtgg aagaaaaatt ccgcgttccc gccgaggtcg tggagcattt
     1801 ccgtcaaggg attggcacac ggggccgaag gcttcgcgaa caatgggaaa agaaattcgc
     1861 ggaatacgaa aaacagtatc cccaagaggc cgacgagatt cgtcgcatcc tcaacggtga
     1921 gctacccgac gggtgggata gcgagattcc caccttcgcc acagatgcta agggattggc
     1981 gacgcgggcg tcctctggga aagtcctaaa tgccgttgcg aaacgcattc cgtggatgat
     2041 cggcggctcg gcggacttgg ctccgtccaa caacacgctc ctgacttttc cggatgccgg
     2101 acacttttcg gcggagaatt atgccgggcg gaaccttcac ttcggcatcc gtgagcatgc
     2161 catgggggct gtggcgaatg gtttggcgtt gtgtggcttg cgtccgtatg ccggcacgtt
     2221 ctttgtgttc agcgattaca tgcgtccggc catccggttg gcggcgctga tggagttgcc
     2281 gattctctat atctttaccc acgattcgat tggtgtgggg gaagacggcc cgacacacca
     2341 gcctgtggaa cacctggcgg ccttgcgggc cattccgggt ttgattgtgg ttcgaccggc
     2401 ggatgcgaac gaggtcgctg aggcgtatcg gctggccttg caagttcgac gaaacccggt
     2461 ggctttggtg ctcacgcgac agaatctttc caccatcgat cgaactcagt atgcggcggc
     2521 cagtgggctt cgatgtgggg cctatatcct ggcggatgcc gagggcggga agcccgacgt
     2581 gattctgatc ggaaccggaa gcgaagtctc tgtatgtctg gccgcgcgcg agttgctctc
     2641 cgcccagggc atcgccgccc gggtcgtaag tatgccaagc tgggagttgt tcgaaaacca
     2701 accggaggag taccgtcatt cggtgttgcc accagaggtt actacccggg ttgcagtgga
     2761 agccggcgta gggttaggct gggagcgcta cgtagggcca catggggcct ttgtaggcat
     2821 gcggagtttt ggggcctctg caccagccgg ggtgctcatg aagtactttg gcatcacggc
     2881 cgaaaacgtg gctaacgtgg ccaaggcctt gatcaagcag gattgaccag agacaagcag
     2941 gcgtgattgg gcgactaaga gcctatccca taatctcccc tcacgcgttc ggcgggagaa
     3001 gggccgtgga gaggattgcg cgaatcgctg ccg
//
LOCUS       DAOPTR010000439         3027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_25942_length_3027_cov_3.767160, whole genome shotgun sequence.
ACCESSION   DAOPTR010000439 DAOPTR010000000
VERSION     DAOPTR010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25942_length_3027_cov_3.767160"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /locus_tag="PKI05_09020"
     CDS             <1..1240
                     /locus_tag="PKI05_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52387.1"
                     /translation="IWELLNEQHASEMWYAQIIPYLKSIDPYQKPVATNWERPQLEGI
                     DINAPHWYQNENELDSDLVTAQRIARDKRFGKPVVYGEQGNSRGQQDRTAEGIGGVWD
                     PGSARRMRVRLWTALFNEAALIFWETSYAKDGHYMNLWIGPEERQYVKALQTFSAGLE
                     GNVRPREVLLSGRDAKDVRGYGLASPSCQAVYLHHFACQQCKDEGKPPAQHRWDHKRG
                     TVSDLAVELESFKTLSAYWYCPQTGKILQRFRISAGRREYAVPPFEVDIALLATEKPA
                     PDSDRDDLANDEDPDDDNDGVPDDQDAFPLEREEWADVDGDRIGDNFDADIDGDGVGD
                     DRNGNGIPDYQEMDADGDGVPKANAIPWDAFPHDPHEWRDTDGDGIGDNADDDDDADG
                     FTDQEERKARTDPLNPLSFP"
     gene            complement(1429..>3027)
                     /locus_tag="PKI05_09025"
     CDS             complement(1429..>3027)
                     /locus_tag="PKI05_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52388.1"
                     /translation="AHQAFPQNRIARMYLGEGIPWTRNWEAVPEAPEWAVHQRVALEG
                     LADIIEWWLDHRQQPDGAFGGGWGDDCEMWRFWVPGLIGFDDPKICAGQKRFSEALFQ
                     QPHMAAGYMSRMTDVEHSAEDSTDTILPMMLLEPDNLVWQQRVLRLAELMENLWTGRN
                     ERGFRQFKSTYFTVDRVHPDPQRACDTPYHVRAVQPVFLYWLRTGDQRVGRVLCEWTD
                     TWVDAAARSENGKPAGVLPATIHWPDGSIGGLSPHWWDPRNHSEPTLYQWPSAMGYLT
                     DALLLTFHMTGDEKYLRPIHSMAELALRYSGRNLPKDIEPGSEAWCALQMGFLRQTLA
                     KYRFLTGDGTWDRLLEGRQAPWLHYALTGDEQPLVAALADTATAFSYNFPGYTSEVRY
                     TDRVLRLPAVFQGDVVLAEPKFPVQSPDTALLYSAVTGDPGGLGTFPLNAVRWLTRPR
                     EIAVLVRMAAPDGLEATLFHFGRSPRAMGAELNMLKPGDYTLKIIARDHVLKEEAFTV
                     SGPRTRIAFEIPSRQSCRLVIRRN"
BASE COUNT          693 a          855 c          851 g          628 t
ORIGIN      
        1 tatttgggaa ctccttaacg aacaacatgc ctccgaaatg tggtacgccc agataattcc
       61 ctatttgaaa tcgatcgacc catatcaaaa accggtggcc acaaattggg agcgcccaca
      121 attggaggga atcgatatca atgcgccaca ttggtatcag aacgaaaacg agcttgactc
      181 ggacctggtc actgcccaac gcattgctcg tgacaaacgc tttggcaaac cagtcgttta
      241 cggtgaacag ggtaatagcc gaggccaaca ggatagaaca gccgagggga tcggtggggt
      301 ctgggaccca gggtcagccc ggcggatgcg cgtccgttta tggacggccc ttttcaacga
      361 agctgcgctc atcttttggg aaacttctta cgcaaaagac gggcattaca tgaatctctg
      421 gattggtccc gaagaaaggc agtacgtcaa ggccctgcaa accttttccg cggggctgga
      481 ggggaatgtc cggccgcgtg aggtcttgct gagtgggcgc gacgctaagg atgtgcgagg
      541 gtatggtttg gcctcgccga gttgccaggc ggtttacttg catcactttg cctgtcagca
      601 atgtaaagat gaagggaagc cgcctgctca gcaccgttgg gaccacaagc gagggacggt
      661 aagtgatttg gccgtagagt tagagtcgtt caagactctg tccgcgtatt ggtattgtcc
      721 ccagacagga aagatacttc agcgcttcag aatttcagca gggagaagag aatatgcggt
      781 ccctcccttc gaggtggata ttgccttgtt agccacggaa aagcccgcgc ccgattctga
      841 tagggacgac ctagccaatg atgaggatcc cgatgatgac aacgatggcg tccccgatga
      901 ccaagatgcc ttccccctgg aacgagaaga gtgggccgat gttgacggag accgcatcgg
      961 ggataatttt gatgccgata tcgatgggga tggtgtaggg gatgatcgca acgggaacgg
     1021 gattcccgat tatcaagaga tggacgccga cggcgacggt gttcctaagg ccaatgccat
     1081 cccatgggat gcttttccgc acgatcctca cgagtggcgc gacaccgacg gcgatgggat
     1141 aggcgacaat gccgacgacg atgacgacgc cgacggattc accgatcaag aagaacgcaa
     1201 ggcccgtacc gacccattga atcctcttag ctttccatag tccttagccg ttgccccact
     1261 ccaaccagtg gagaaaggca gacgcgactc ggcatggctg aatatgagcc tgctaaaaca
     1321 ggcggtgttt agtttgccat ccatgttgcc acttccctat tgggctgctt ttgcaatgca
     1381 ccctcgtgtc tttctgtgtc tgcgatgagt tcaagtgccg ggatcgtttc agttacgtct
     1441 aatgactaac cggcacgatt gccgcgaagg aatctcgaag gcaattcggg tgcgcggacc
     1501 ggaaaccgtg aacgcttcct ctttcaaaac atgatcccgg gctatgattt ttagcgtgta
     1561 gtcgccgggc ttaagcatat tcagttctgc ccccatcgcc cgcggtgatc gaccaaagtg
     1621 aaacaatgtg gcctccaaac cgtcaggagc tgccatcctg accaaaaccg caatctccct
     1681 ggggcgcgtt agccagcgca ccgcattaag agggaaagtt cccaaccctc ccggatcgcc
     1741 cgtcacggcg gaataaagca atgcagtatc cggggattgg accggaaatt ttggttcggc
     1801 caataccacg tcgccttgaa acacggcggg gagcctcaat acgcggtcgg tgtagcggac
     1861 ttcgctggtg taaccgggaa aattgtagga aaaggccgtg gccgtgtccg ccagggcggc
     1921 cacaagtggc tgctcatccc ccgtaagtgc gtagtgaagc cagggtgcct gtctcccttc
     1981 cagcaagcga tcccaagtcc cgtcaccggt gaggaagcgg tacttggcca gtgtctgccg
     2041 caaaaatccc atttgcaggg cacaccaggc ctcgcttccc ggctcgatgt ccttgggaag
     2101 gtttcgccca gaataccgca gggccaactc cgccatggag tgaataggac gcaggtattt
     2161 ttcgtctcct gtcatgtgga atgtgagcag caaagcatcg gtgaggtaac ccatcgcact
     2221 gggccactgg taaagcgtcg gctcactgtg attgcgcgga tcccaccaat gcggtgacag
     2281 gcccccgatg gacccgtccg gccaatggat ggttgcgggc aagactccag ccggctttcc
     2341 attttctgat cgtgctgcgg catccaccca tgtgtccgtc cactcacaca aaactcgccc
     2401 gacccgctga tcacccgtcc gcaaccagta caaaaaaaca ggctgcactg cgcgaacgtg
     2461 atacggagtg tcgcatgccc gctgaggatc aggatgaacg cgatctaccg tgaaataggt
     2521 acttttgaac tgccgaaatc cccgctcatt gcggccggtc cagaggtttt ccatcaactc
     2581 cgctaggcga aggacccgct gctgccacac caggttgtcc ggctccaaaa gcatcattgg
     2641 caagattgtg tcggttgagt cctcggcact atgttcgacg tcggtcattc tggacatgta
     2701 cccggccgcc atgtgcggtt gctgaaaaag ggcttccgaa aagcgcttct ggccggcaca
     2761 gatcttgggg tcatcaaatc caatcaaccc ggggacccaa aagcgccaca tttcgcagtc
     2821 atcaccccaa ccgccgccaa aggccccatc cggctgctgc cgatgatcga gccaccactc
     2881 gatgatatcg gctaggcctt ccagtgccac ccgctgatgg accgcccact ccggagcctc
     2941 aggcactgct tcccaatttc gtgtccatgg aattccctcg ccgagataca tccgcgcaat
     3001 gcggttttgt ggaaaggcct ggtgggc
//
LOCUS       DAOPTR010000440         3024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_25986_length_3024_cov_3.869990, whole genome shotgun sequence.
ACCESSION   DAOPTR010000440 DAOPTR010000000
VERSION     DAOPTR010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25986_length_3024_cov_3.869990"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PKI05_09030"
     CDS             complement(<1..710)
                     /locus_tag="PKI05_09030"
                     /inference="COORDINATES: protein motif:HMM:NF014048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HOA52389.1"
                     /translation="MPVEVAMWRLGEKLSPVGFSSIDRESRLEELLADDISIVDPNLL
                     LIGRQVPTPYGQFVDLLAIDSDGNLVVIELKRNRTPREVVAQLLDYGSWVRSLKADDV
                     AEIFGDFLTKYYPQHVNTSLDSAFCERFGAEEMPEGINESHELMLVAGELDASSERII
                     TYLAEEYGVAINAVFFRYFRDGDREYLSRAWLIDPRDVDVKVEEKRTNLPWNNEYYVS
                     FGHKDDGTSRHWEDAQKYG"
     gene            917..1294
                     /locus_tag="PKI05_09035"
     CDS             917..1294
                     /locus_tag="PKI05_09035"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1937 family protein"
                     /protein_id="HOA52390.1"
                     /translation="MIYLASPYSHPDAAVRQQRFESACRAAAELIRQGYVVFSPIAHS
                     HGIAQHGLPLDWGYWERHDRRLLAACDELWVLMLDGWEDSRGVQAEVAIAQAMGKPVR
                     FVSTEYHNPASAYQYCCSNPDCH"
     gene            complement(1423..2082)
                     /locus_tag="PKI05_09040"
     CDS             complement(1423..2082)
                     /locus_tag="PKI05_09040"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HOA52391.1"
                     /translation="MCGIFGFLSTNGRGPDLACLRRIAAETETRGRHAFGLAWIDVEG
                     ELRTFKRPGAATACLDDLDACRHAVAVVGHCRWATHGDPQDNRNNHPHPAGRGWLVHN
                     GVVRNYAELLRRYGLRPRTACDSEVLGLLMMRRAGPLVERAAWAVAQTEGPLALLGLW
                     GDPVRLLVVRRGNPLCFSETPGGLYFASLPAGLPGKVFSVADNHVSVIQSKRITIRHL
                     R"
     gene            complement(2116..2967)
                     /locus_tag="PKI05_09045"
     CDS             complement(2116..2967)
                     /locus_tag="PKI05_09045"
                     /inference="COORDINATES: protein motif:HMM:NF023645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidoligase family protein"
                     /protein_id="HOA52392.1"
                     /translation="MNAHDLTFGIEIETIAPDSAVRNDRLRIGPYKRGIQVPYLPAGW
                     KAEADGSIDNSNGGHRCEIVSPVLRGEEGIRQVIEVLRTLEAKGHRVNVSCGVHVHVG
                     WNRDWPSEALARLVTIVAYLEKGLYAITGTKNRERGRYCGGVRKYGSDKDAKPALDRD
                     RYHALNLTNLARGTKDTVEFRVFSGSLNPTKVVGWIQVCLGLVERALTAKRMPTWNPK
                     APTGGWKKSGPGQSETERLLGYLAWGKGYARIHGGKQYGWISDAIPQDQIKAEFRRLA
                     KKYDAMP"
BASE COUNT          536 a          910 c          966 g          612 t
ORIGIN      
        1 ccgtatttct gtgcatcctc ccagtggcgg ctggtaccgt catccttgtg tccaaaggaa
       61 acgtaatact cgttgttcca cgggagattt gttcgtttct cttcaacctt gacgtctacg
      121 tcccgggggt caatcagcca cgcacggctg aggtactcgc gatctccgtc ccggaaatat
      181 cggaagaaga cagcgttgat ggcgacccca tattcctcgg ccagataggt gatgatccgc
      241 tcgctactcg cgtccagttc accagcaacc aacatgagct cgtgcgactc gttgatgcct
      301 tctggcatct cctctgcacc gaagcgttcg cagaatgccg agtccaggga tgtgttgaca
      361 tgctggggat agtacttcgt caggaagtcg ccaaagattt cggcaacatc atccgctttc
      421 aagctgcgca cccaggaacc gtagtccaac aactgggcga caacctcccg tggcgtgcga
      481 ttacgcttca gttcaatgac cacgagattg ccgtcggaat cgatcgccag gaggtcaacg
      541 aactgaccat agggcgtggg aacctgtcgc ccgatgagca gcaagtttgg gtcaacgatg
      601 gaaatatcgt cagcaagcaa ctcctccaac cggctttcgc gatcgatcga cgagaagccg
      661 actggactga gtttctcgcc cagccgccac attgcgactt cgacaggcat gaatctctct
      721 ccttcaatac gcgaggaacg ccttcttcgg aggcagaagt tttgccacct accgacagtc
      781 tagcctctct gtctgctgga aacaattcct tcgtgaatca tggcccatgc ccctggtgtc
      841 accggaacga actccgggac cgtgagcgcc ggtgtcgcgc ggtcgaagag ggcatctcgg
      901 aaaggaaaga gcaacgatga tttatctcgc gtcgccctac tcgcatcccg acgcggccgt
      961 gcgtcaacag cgcttcgagt ccgcttgccg tgcggcggcg gaactcatcc ggcaagggta
     1021 tgtggtgttc tcgccgattg cccacagtca cgggattgcc cagcacggct tgcccttgga
     1081 ttggggctac tgggagcggc acgatcggcg gctcttggcc gcttgcgacg agctttgggt
     1141 gcttatgctc gatggctggg aggacagtcg gggcgtgcag gccgaagtgg ccatcgccca
     1201 agcgatgggg aaaccggttc gctttgtcag caccgaatat cataacccgg ccagtgctta
     1261 ccagtattgc tgctctaacc cagactgcca ttagccgttt taggggcata ggatcgtttt
     1321 agacgcgatc gcgctactgg cgcgagaaga aataggtaaa gagtgttgcg tgcgttttgt
     1381 gggccaaaat tggcgatttg gtggcgtgct acagagaata ggctagcgaa gatggcggat
     1441 ggtgatcctt ttcgactgaa tcacactcac atggttgtca gcaaccgaga acaccttgcc
     1501 tggaagccct gcgggcaggc tggcgaagta gagtccgccg ggcgtttcgg agaagcagag
     1561 cgggttgccc cggcggacga ccaacagccg cacgggatcg ccccacaggc cgagcaaggc
     1621 caaggggcct tccgtttggg ccacggccca ggcggcccgc tccacgagcg gcccggcccg
     1681 gcgcatcatg agcagtccca gcacctcgct gtcgcaggcg gtcctgggcc gcaggccgta
     1741 gcggcgcaga agctcggcgt agttgcggac gacgccgttg tggaccagcc agccgcggcc
     1801 ggccgggtgg ggatggttgt tgcggttgtc ctggggatcg ccatgggtgg cccagcggca
     1861 atggcccacc acggccaccg cgtggcgaca agcgtccagg tcgtccaggc aggccgtggc
     1921 tgcgcccggg cgcttgaagg tccgaagctc cccctcgacg tcgatccacg ccaggccgaa
     1981 ggcgtggcgg ccacgggtct ccgtctcggc ggcgatccgg cggaggcagg ccagatcggg
     2041 gccgcgtccg ttggtactca agaatccaaa gatgccgcac atggtggatg gcctttctta
     2101 ctggcggttt gcgttttagg gcatggcgtc gtacttcttg gccaggcggc ggaactcggc
     2161 cttgatctgg tcttggggga tggcgtccga aatccatccg tactgcttgc ccccgtgaat
     2221 ccgggcgtag ccttttcccc aggccaagta gcccaggagg cgttccgtct cgctctggcc
     2281 cgggcccgac ttcttccagc cccctgtggg ggccttgggg ttccaggtcg gcatccgctt
     2341 ggccgtcagg gcccgctcga ccagccccag gcagacctga atccagccga cgaccttggt
     2401 ggggttgagc gagccggaga agacgcggaa ctcgaccgtg tccttggtgc cgcgggcgag
     2461 gttggtgagg ttcaaggcgt ggtagcgatc gcggtccagg gcgggcttgg cgtccttgtc
     2521 gctgccgtac ttccgtaccc cgccgcaata ccggccccgc tcgcggttct tggtgccggt
     2581 gatggcgtaa aggcccttct ccaggtaggc gacgatggtg accaagcggg ccagggcctc
     2641 gctgggccaa tcgcggttcc aaccgacgtg gacgtggacg ccgcagctca cgttgacccg
     2701 atggcctttg gcttccagcg tgcggaggac ctcgatcacc tggcggatgc cttcttcgcc
     2761 gcggaggacg gggctaacga tttcgcacct gtgacccccg ttgctgttgt cgatcgagcc
     2821 gtcggcctcg gccttccagc cggcgggaag gtagggaact tggattccgc gcttatacgg
     2881 gccgatgcgg aggcggtcgt tgcggacggc gctatccggg gcgatcgttt cgatctcgat
     2941 tccgaaggtc aggtcgtggg cgttcatggc ttttccctcg ctagtgtgat gttcgtgcgt
     3001 acatcacact taagccatga actc
//
LOCUS       DAOPTR010000441         3023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_26000_length_3023_cov_3.273922, whole genome shotgun sequence.
ACCESSION   DAOPTR010000441 DAOPTR010000000
VERSION     DAOPTR010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26000_length_3023_cov_3.273922"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="PKI05_09050"
     CDS             complement(<1..889)
                     /locus_tag="PKI05_09050"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA52393.1"
                     /translation="MSRGKQASGGDLSRRDFLKTGAVAAGGMAFGTALHRVVHASGSD
                     IIKVGMIGCGGRCSGAAAQALNTGPDVRLVAMTDVFESKVQAALQNLKQQFPDKVVVD
                     QDHCFTGLDGYKKVIEASDVVLIACASKYHPMYSEEAIKAGKHVFVEKPHGIDPVGVR
                     RMHAACDLAKEKGLSIVSGLQSRFDIGWQECMKRIHDGIIGDIVAVQAMFLRGPYQVM
                     TRKPGQSEVEFQYWNWYHFCWLSGDDVPQSLVHNMDRVSWALKEEMPEWCFGLGGRSA
                     SFGEGLGDMYDHQTVVYEYK"
     gene            complement(981..1859)
                     /locus_tag="PKI05_09055"
     CDS             complement(981..1859)
                     /locus_tag="PKI05_09055"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOA52394.1"
                     /translation="MQISFHTDAFNSAYWNFERCLKWAEANDVHFIECGVIDGVSWIH
                     GLGYQPHVALYEDPLLLRRKMENYGVRFSQIDAAYPLSGRDGPLYGVPYVLKAIPWAK
                     HAGCPRIATTDGLHQPPGISDEEAMALMKRSYEQIIEVAEAYEITINIEVHGYFTTNP
                     DRLAEMLEFCQSPFLRMNLDTGNSFIAGRDPVEFCRRFREKINHVHVKDVSSSLAAAV
                     RGQETGIAVSHCAIGDGVNAENIRQVLMILRDYGYNGPLSMECEGQGGPMIERSLRWL
                     RATLQELGIPEEKSVP"
     gene            complement(1974..2744)
                     /locus_tag="PKI05_09060"
     CDS             complement(1974..2744)
                     /locus_tag="PKI05_09060"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOA52395.1"
                     /translation="MMCRWYSVLALVLSLMPFRAIDTFGEEQGTVGQSQEKAPVRCLI
                     VTGEDYPGHKWRETTPVLKSALERQGQISADVAEDLNVLRTDQPYQYDVIVMHFKNYD
                     PNVPGREGFQKLAAFVRQGGGLVLVHFACGAFQEFKDDFVQIAGRVWDPQLRGHDPHG
                     TFTVKIVDPQHPITHGMKDFETVDELYTCLAGEVAIHILATAKSKVDGKDYPMAFVLE
                     VERGRTFHCVLGHDVKAFASPEVQELLRKGCLWATRRL"
BASE COUNT          731 a          925 c          707 g          660 t
ORIGIN      
        1 tcttgtactc gtaaacaacc gtctggtggt cgtacatatc gccaagccct tcgccaaagg
       61 aggccgaccg accaccgagg ccaaagcacc attcgggcat ttcttctttc aaggcccaac
      121 tgacacgatc catattgtgt accagagact gtggcacatc atcaccggaa agccaacaga
      181 agtgatacca gttccaatat tggaactcga cttctgattg tccgggtttg cgggtcatga
      241 cctgatacgg cccacgaagg aacatggcct gcaccgcgac gatatcgcca atgatgccat
      301 catggatgcg cttcatgcat tcctgccagc caatgtcaaa ccggctttgc aggcccgaca
      361 caatgctaag acccttttcc ttcgctagat cacaagccgc atgcattcgg cgaacaccca
      421 ccggatcaat accatggggc ttctcgacga agacgtgttt gcccgccttg atggcctctt
      481 ccgagtacat tggatgatac ttggaggcac aagcaatcaa gaccacgtca gaggcctcga
      541 tgaccttttt gtaaccgtcc agaccagtga aacaatgatc ttgatcgacg accactttat
      601 cagggaattg ctgcttgaga ttctgaagcg ccgcctggac tttgctttcg aacacatccg
      661 tcatggcgac cagccgcaca tcgggccctg tattgagtgc ctgagcggcc gccccgctac
      721 agcgaccgcc acagccaatc atccccacct taataatgtc cgagccactc gcgtggacca
      781 cacgatggag tgccgttcca aaggccatcc cccccgcggc cacggcccct gtcttcaaga
      841 aatcgcgacg cgacaaatca ccaccagacg cctgcttgcc acgactcata acatggtccc
      901 tccaaatcaa ctgagccaac caaacggacg atcttcacgg atatacaaca tcaccaattt
      961 gccccggatg accaccttcc ttacgggacg gacttttctt cgggaattcc caattcttgc
     1021 aaagtcgccc gcaaccagcg aagcgatcgc tcgatcatag gaccgccttg cccttcgcat
     1081 tccatactca gcgggccgtt gtaaccgtaa tcccgcaaga tcatcaagac ctggcgaatg
     1141 ttctcggcgt tgacgccatc gccgatcgca caatgactta cggcaatgcc ggtttcctgt
     1201 ccgcggacgg ctgcggccaa tgaagaactg acatctttca cgtgaacgtg gttgatcttc
     1261 tcacgaaagc gtcggcagaa ttccacggga tcacgtccgg cgatgaacga attccccgtg
     1321 tcaagattca tccgcaggaa aggactttga cagaactcca acatctcggc caagcgatca
     1381 ggattggtcg taaaatagcc gtggacctca atgttgatcg tgatttcata ggcctcagca
     1441 acctcaatga tctgctcgta acttcttttc ataagggcca tggcctcttc gtcactgatg
     1501 ccgggaggtt gatgcaaccc atcagtggtg gcaattcggg ggcacccagc atgctttgcc
     1561 cagggaatcg cttttaagac atagggaaca ccataaagcg gtccatcccg accggaaaga
     1621 ggataggcag catcaatttg tgagaaccga acgccgtaat tctccatttt tcgccgcagg
     1681 agcaatgggt cttcatacag ggcaacgtgc ggctgatagc ccaacccatg aatccaactc
     1741 acgccatcaa tcacgccaca ttcaatgaaa tggacatcat tagcctccgc ccatttcaaa
     1801 caccgttcaa aattccaata agcggagtta aaggcatcgg tatggaaact gatctgcatc
     1861 gcatcgactc ccttgtctct tgtcaagcac caaagctttt cgcggcctcc cgtcattttg
     1921 gagacggcca acaccagctc ttaggctatt ccctgggtgt gaccccatct aggtcacagg
     1981 cggcgtgttg cccaaaggca acccttccgc aaaagctcct ggacttccgg cgacgcaaag
     2041 gctttcacat catgccccag cacacagtgg aaggtgcgtc ctctttctac ctccagcacg
     2101 aaggccatgg ggtagtcctt tccatccact ttcgacttcg ctgtggcaag gatgtggatt
     2161 gccacctcac cggccaaaca tgtatagagt tcgtccaccg tctcgaagtc tttcatgccg
     2221 tgcgtgatgg gatgttgggg gtcaacgatc ttcaccgtaa acgttccgtg gggatcgtga
     2281 ccacgaagtt gcggatccca cacacgacca gcaatttgaa caaagtcatc tttgaattcc
     2341 tggaaagcgc cgcaggcaaa atgcacaagg accagcccac ccccctgccg aacaaacgcg
     2401 gccagcttct ggaacccttc gcgacctggc acattcggat cgtagttctt aaagtgcata
     2461 acgattacgt cgtattgata cggttggtcc gtccgcaaga cattcaggtc ttcggcaacg
     2521 tcagcgctga tctgcccctg acgttccaaa gcagacttta agaccggcgt tgtctcccgc
     2581 catttgtgtc cgggataatc ctcgcccgta acaatgagac aacgaacagg ggccttctcc
     2641 tgagattgtc ccacagttcc ctgttcctcg ccgaacgtgt ctatcgccct aaaaggcatc
     2701 aggcttaaca ccagggccaa cacactgtac caacgacaca tcatggccac gatcccttct
     2761 tgagcaagtc acacgacggc gccctaatgc gagggactcc cgatctcttg cataacgcgc
     2821 tcgaagtatt atctctctcg aaacacttcg accgatagca tagccgacgc ttttgccccg
     2881 tcaagcatgc gttccagttt ttcagcaaat cgagtcaccg caaaaaaata acccgcaggc
     2941 tttcctgcgg gttaccctaa gcatagtctc ccctcgcccg ttcggcggga gaggggtcgg
     3001 gggtgagggt ggctcgaatc gct
//
LOCUS       DAOPTR010000442         3020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_26047_length_3020_cov_2.968634, whole genome shotgun sequence.
ACCESSION   DAOPTR010000442 DAOPTR010000000
VERSION     DAOPTR010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26047_length_3020_cov_2.968634"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1731)
                     /locus_tag="PKI05_09065"
     CDS             complement(<1..1731)
                     /locus_tag="PKI05_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOA52396.1"
                     /translation="MKEALEELQQAILAHVRSEKYQPVKPRVIAKALKIPAEEAHLVK
                     KAVKALVKAGFLEFGSGHLVYLAGTRKSAKPIEQVSGIIGVFRRKEAGHGFVRPKPQP
                     GQTEPLEEIFIPPEWSSDAATGDLVRVRLAKRKRGRDLGPAGQIVEILERQSSRFVGT
                     YFEADGQGYVQVDGTVFAQPIYVGDPGAHGVQPDDKVVIEMLRFPNHFRQGEGVIVEV
                     LGPLGKPGVDTLTIMREFNLPEEFPPEVLEEARQVAQAFEETIPPDRYDATAETTITI
                     DPEDARDFDDAVSLERLADGSWRLGVHIADVAYFVPPGSLLDHEARNRGTSVYLPDRV
                     IPMLPELISNGLASLQPGKLRLTKSVYMEFTPEGIKTDVKIERSVIRSDRRLTYEQVD
                     AFFEDPAAYKRAWGTKVHDLLLRMRELARILRQRRRDRGALELVMPEVKVDLDRRGRV
                     VGAHLVPNTESHQIIEEFMLAANESVAETLRDRGWLFLRRIHRPPTARKLRQLQDFVE
                     ALAFPSRDLEDRFGLQSLLEMTKGKPQQYAVHYAVLRSLQRAIYSPLEEGHYALASEC
                     YCHFTSPIRRY"
     gene            complement(1977..2396)
                     /locus_tag="PKI05_09070"
     CDS             complement(1977..2396)
                     /locus_tag="PKI05_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOA52397.1"
                     /translation="MCHEISQQPLTDATTSLSQLRQLVAEFIRARAWEPFHTPKNLVM
                     ALAIEAAELMEHFQWLTPEQAEEIKTDQAKRSEVADELADVLCYVMALANRLEIDLSA
                     VVHAKMLKNARKYPVEQFRGYFGPEDPRWPGRTSTEH"
BASE COUNT          656 a          860 c          844 g          660 t
ORIGIN      
        1 atagcgccgg atgggcgacg tgaagtgaca ataacattcg ctggcaaggg catagtgtcc
       61 ttcctccagc gggctgtaga tggcccgttg aagggaccgc aaaaccgcat aatgcacggc
      121 atattgttgg ggcttaccct tagtcatttc cagtaaagat tgcagaccga agcgatcctc
      181 caggtcgcgg gaaggaaacg ctagggcctc aacaaaatct tgcaattgcc gcaatttgcg
      241 ggcggtaggt ggtcgatgaa tccgtcgcag gaataaccaa ccgcggtcgc ggagcgtctc
      301 cgccacgctc tcgttcgccg ccagcatgaa ctcctcgatg atctggtggc tttcggtatt
      361 gggcacaaga tgggcaccaa cgacccgacc gcgacggtcc aaatcgacct tgacctctgg
      421 catgaccaat tccaaggcac cgcggtcacg tcggcgttgg cgcaagattc gggcaagctc
      481 ccgcatccgc aagaggagat cgtggacctt cgttccccac gcccgcttgt aggccgccgg
      541 gtcctcaaag aaggcgtcca cctgttcata ggttagccgc cgatcgctgc ggatgaccga
      601 tcgctcgatt ttgacatccg tcttgatccc ttctggggta aactccatat aaacggactt
      661 cgtcagtcgc agtttcccgg gttgcaaact ggccaagccg tttgatatta gttccggcaa
      721 catggggatc acacgatccg ggagatagac gctcgtcccg cggtttcgtg cctcgtgatc
      781 taacagggat ccgggcggca cgaagtaggc cacgtcggca atatggaccc ccaaccgcca
      841 actaccatcc gccaggcgtt cgagcgacac ggcgtcgtcg aagtcgcggg catcttctgg
      901 gtcaatggtg atggttgttt ccgccgtggc gtcgtaccgg tccgggggaa ttgtctcttc
      961 gaaggcctgg gcaacctgcc gggcttcttc taggacttca ggcggaaact cctcgggaag
     1021 attaaattcc cgcatgattg tcagcgtatc gaccccaggt ttccctagcg gcccaagaac
     1081 ctcgacaatg actccctcac cttgccgaaa atgattgggg aagcggagca tttcgataac
     1141 cactttgtca tcaggctgca cgccatgggc acctgggtcc cccacataaa tcggctgggc
     1201 gaacaccgtc ccgtcaacct ggacataccc ttgcccatcg gcctcaaagt aggtccccac
     1261 aaagcgagag ctttgtcgct ccaggatttc cacgatctgt ccggcaggac caagatcacg
     1321 gccacgcttc cgcttggcca gacgcaccct cacgagatcg cccgtcgcgg catcgctcga
     1381 ccattccggc ggtataaaaa tctcttccaa tggttctgtt tgtcccggtt ggggcttggg
     1441 gcggacgaag ccgtgaccgg cttccttacg acgaaaaacc ccaatgatgc ccgagacttg
     1501 ctcgatcggc ttggctgact tccgagtgcc cgctaaatac accaagtggc cagaaccaaa
     1561 ctccaagaag cctgccttta ccaacgcttt gacggctttc ttgacaagat gggcctcctc
     1621 agccgggatt ttcagcgcct tggcgatgac gcgaggtttc accggctggt atttctctga
     1681 acgcacatgc gccaggattg cctgttgtaa ttcttccaac gcttctttca tctaatcaca
     1741 ctttctcaaa aaactctgcg caattgctca cagagactgg aagagcgcag actcccaaag
     1801 aagtcgccca ccttaagtct cttaaagaag gacactccaa gacagggcga gacaatcgca
     1861 atgcgagggc acatctggcc gccagcctcg tggacagcct gggggattcc ttggaaaagt
     1921 cccttttcaa cgtttgacat ctgctttcct taaatatacg ctcgtcaagt gataattcaa
     1981 tgttcggttg atgtacgccc tggccaccgg gggtcctccg ggccaaagta ccctcgaaat
     2041 tgttccaccg ggtatttcct ggcgttcttg agcatcttgg cgtgcaccac ggcggagaga
     2101 tcaatttcaa gacggttggc caaggccatg acatagcaca gaacgtctgc cagttcgtcg
     2161 gccacctcgg aacgtttcgc ttgatccgtc ttgatttcct cagcctgttc gggtgtcaac
     2221 cactgaaagt gctccatcaa ctcggcggct tcaatggcca aggccatcac caagtttttt
     2281 ggggtgtgaa aaggctccca cgccctcgcg cggatgaatt ccgccacaag ctgccgaagc
     2341 tggctaagag aagtcgtagc atccgtcaac ggttgttgag atatttcatg gcacatcgca
     2401 acgtccacgc ctaagatggt gcgactctag gatactcacc tcccctgcat cataatggaa
     2461 agacgaagcg gtgtccgcat gccctgcacg aacctacctt catgagatga cttgcgggcg
     2521 tttagggcgg atgaagacgc aaagtctcgc atcaaggaat ctcgccgcgt ttggtggccc
     2581 atagaagata accttggtat gttcggccgg ttgtttggga atcctctctg agtgttggcg
     2641 atctgtgctg gctgacggta aactaggaga cttggggagt atgcagagtg tgactccccc
     2701 gtctttccgg tggatatggc ggcggaaaag gtggaacaac gggcgcaaaa ggagctgagg
     2761 gagaccctcg gggccacggt gacataggcc aatcggtctg caaatggggt acagggagcc
     2821 gttgatcgga gcgatcgctt gtggctctgc cggagtgttt tgtggtcgta cggtgtggtt
     2881 gggttggccc caatcgggcg caacccacgg ggacagagtc tggtcgcgat gcaatgagca
     2941 cggcttaaag cctatcttaa aaggtcccct ctccctccgg gagagggttg gggtgagggc
     3001 aagcgaacgt cggaatcgat
//
LOCUS       DAOPTR010000443         3018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_26066_length_3018_cov_3.519406, whole genome shotgun sequence.
ACCESSION   DAOPTR010000443 DAOPTR010000000
VERSION     DAOPTR010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26066_length_3018_cov_3.519406"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            748..2346
                     /locus_tag="PKI05_09075"
     CDS             748..2346
                     /locus_tag="PKI05_09075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA52398.1"
                     /translation="MAITEVDLSGRNRARRQDCETPITVTCRVAQDTTSQPSEAKTYL
                     GERQETVANLSQKCQKLSVIVPVYNERWSLAEVIRRVLEAPVTLEKEVIIVDDGSTDG
                     SYEVALELAAQDRRIQVIRHQRNAGKGAALRAGIEAVSGDVVIIQDADFEYDPTDYPT
                     LLEPILQGKADAVYGSRFVGHCRRVLFFWHTLANKFLTLVSNMVNDLNLTDMETGYKA
                     IRTDVLRSLRLTANTFTIEPELTCRLAQCGARIYEVPISYTGRTYEEGKKIRAWDGLK
                     ALAAIFYYRFIDTQYTHDEALATLQSVKKAKAYQRWLIDMVRPFLGKRILEAGAGIGN
                     LSTLLLNAERLILVDRNPRYCEILRQRFSGRANISVELADLADIELYQHHRLDGLDTV
                     FCANVLEHIADDQRVLSHFYQVLEEGGRCILIVPAGPKLFGSLDEAVGHFRRYSEHDL
                     VQKVRAAGFEVVHCRRFNRLGSLAWFISSRVLRRRTLSPHAMKWFSRLVPLAKLLDWL
                     LPVRGMSLVVVGQKPRQQRSKLAA"
     gene            2426..>3018
                     /locus_tag="PKI05_09080"
     CDS             2426..>3018
                     /locus_tag="PKI05_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52399.1"
                     /translation="MRLSLGLCVLGGVLVSASGATLVLAALKITGMVPSLGGVALPHR
                     VGWPMLTLLVGMGLLAAGWPSPKSPLLQRWQDFIRSFSQLWNHGNLIWRIVITALIGH
                     LAISLAYFLGAPVDLHETFVERANLQGTLLSIRAGQRYVHWEFLARLWKETIPPRAAV
                     AYRGHWEGPLVAYELYPRRLYLLPEDGRRLAANWHNHR"
BASE COUNT          657 a          889 c          820 g          652 t
ORIGIN      
        1 tcgattccga cgttcgcttg ccctcacccc aaccctctcc cggagggaga ggggagactc
       61 ttcgggatag actctaaata cgaagaaaca ccgcacgcgt cacccaccag acaacgcaag
      121 cccgcgtgtc cgtcccctgc gtccagcccg ccatggctgg gtcataattc ccccaaagat
      181 aacagggtcc accccagaca tcaacgcgag ggcctaccca ccgttacgca ttcactgacg
      241 aagctcacga aggccgaaga agcctcaagc ctacaaattg ccatatcctc ggaacacgtg
      301 accttaggtt tacaagatcc ctctctccag ctcatccgcc aacaaatcac ctatgttcgc
      361 ccgaagcatc gtaaagcccc ctgtggttgc cccgtttggc gcagggaggg tcgccgtccc
      421 cggcggccaa atcaccgggc aacacggacg cccgtgggaa acaaccgatc aacacggtga
      481 tcgaaaatac cacggccaag atgcctgtgc cacgcacagg gcgcagggca ggcacagggg
      541 cctgccccta cggttttgtt gcggcgcacg taggggcacc cccccgtggt cgccccgctt
      601 ggcgcaggga gggtcgccgt cgtcggcgac caaatcacct ttgctcttga cttttcgctg
      661 tttgtcaaat caaaaggatg tcttataatc aacttcgatg ggacggaacg gtggaaatcg
      721 tacggttaca gcaagggagg gatcccaatg gccatcacgg aagtcgatct atccggccga
      781 aatcgcgcca ggcggcaaga ttgtgaaaca cccataacag tcacatgtcg tgtcgcccag
      841 gacacgactt cgcaaccgtc agaggcaaag acctatttgg gagagcggca agagacggtt
      901 gccaacttgt cgcagaaatg ccaaaaactt tccgtgattg ttcccgttta taatgaacgc
      961 tggtccctgg ctgaggtcat tcgccgcgtt ttagaggccc ctgtaacact tgagaaagaa
     1021 gtcatcatcg tcgacgacgg atcgaccgac ggttcttacg aagtggctct cgaattggcc
     1081 gcgcaagatc gccgaatcca ggttattcgc catcagcgaa acgccggcaa aggggcagcc
     1141 ctccgggcag gcatagaggc cgtctctggc gacgtcgtta tcatccagga cgccgatttt
     1201 gaatacgatc cgaccgatta tcccacgctc ctggaaccga ttctccaggg aaaggccgac
     1261 gccgtttatg gctcccgttt tgtcggccat tgtcgccgcg tccttttctt ttggcacacc
     1321 ttggcgaaca agtttctaac ccttgtttcc aatatggtca acgacctcaa cttgaccgac
     1381 atggagacgg gctacaaggc gatcaggacg gatgtccttc gcagcctgcg attgacggcc
     1441 aataccttta ccattgagcc tgagctgacg tgccgtctcg cccagtgtgg ggcgaggatc
     1501 tacgaagtgc ctatttccta tacaggtcga acatacgaag aggggaaaaa gattcgtgct
     1561 tgggacggcc ttaaggccct ggccgccatc ttctattacc gcttcatcga cacccagtac
     1621 acccatgatg aagccctggc gacacttcag tccgtgaaaa aggccaaggc gtatcaacgg
     1681 tggctcattg atatggtccg cccatttctt gggaagcgaa tcctggaggc cggggccggg
     1741 atcggcaatt tatcaacact ccttctcaat gccgaacggt tgatcctggt agatcggaac
     1801 cctcgctatt gcgaaatact ccggcaacga ttctccggtc gggcgaacat ctccgtcgag
     1861 ctcgcggact tggccgacat tgaactgtat caacaccatc gcctagacgg gctagacacc
     1921 gtcttttgcg caaatgttct ggagcatatt gctgacgatc aacgggtctt gtcgcacttc
     1981 taccaggttc ttgaggaagg cggacggtgc atcttgatcg tacccgccgg accaaagctc
     2041 ttcggctccc tggacgaggc tgtggggcac ttccgccgat acagcgaaca cgatttggtg
     2101 caaaaagtcc gcgcggcggg gtttgaggtc gtccactgcc gacggttcaa ccgattgggg
     2161 agcctcgcgt ggtttatttc atccagggtt ttgcgacggc gaaccttaag ccctcacgcg
     2221 atgaagtggt tttcccgcct cgttcccctg gccaaactgc tggattggct acttcccgta
     2281 cggggaatgt cgctggttgt tgtcggccaa aagccacgtc aacaaaggtc gaaactggcg
     2341 gcataaagca gatgccctat catggtgaat gttagtcctt gtcatggcaa acgtaatgat
     2401 ccacgctgac aaaggacaaa agaggatgag gctttccctt gggctatgcg tcctcggtgg
     2461 tgttttggtg agtgcttccg gcgcgacgct ggttctcgcg gcgctgaaaa taacgggcat
     2521 ggtcccttca ctcggtggtg tcgcgctgcc ccatcgcgtg ggttggccga tgctgaccct
     2581 cctggtcgga atgggcttgc ttgccgccgg ctggccttcg ccgaaaagtc ccctacttca
     2641 acgttggcaa gacttcattc ggtccttttc tcaactatgg aatcacggga atctaatttg
     2701 gcggattgta atcaccgctc taatagggca tttggcgatt tccttggcct attttctcgg
     2761 agcgccggtg gacttacacg aaacttttgt ggaacgggca aatctccagg gaacgctcct
     2821 cagcattcgc gcgggacagc gctacgttca ctgggagttc ctggcacgct tatggaagga
     2881 gacaatacct ccacgggccg ccgtggccta ccgaggacat tgggaaggac cactcgtcgc
     2941 ttacgagctt tacccccgca ggttgtattt gctccccgaa gatgggcgcc gcctcgctgc
     3001 caattggcac aaccaccg
//
LOCUS       DAOPTR010000444         3018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_26068_length_3018_cov_3.152211, whole genome shotgun sequence.
ACCESSION   DAOPTR010000444 DAOPTR010000000
VERSION     DAOPTR010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26068_length_3018_cov_3.152211"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..1285
                     /locus_tag="PKI05_09085"
     CDS             212..1285
                     /locus_tag="PKI05_09085"
                     /inference="COORDINATES: protein motif:HMM:NF027641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HOA52400.1"
                     /translation="MITRRGFLRQTSVLGTASLVPNLLTTGWGLEPNHVPLSREKVGR
                     HILAFYYPWYGNPAASGGSGRWSHWQDVDEQAKTIGSSTHYPELGPYDSHDRKVITQH
                     CRWAVEAGLTGWIASWWGHRSFENQALPRILDISGEHKLAVTIYYETIPGQPKTVDNA
                     VTDIVRLLEQHANHPAWLTVDGKPVIFIYGRAVGEIGVAAWGEVIQKVNAAYPRGAIF
                     QGDQFSAAAAKMFDGLHTYNTAGQLRGKSLEEVKSWCAERYPQWVKLARGAGKISSLT
                     VIPGYDDTKIRKPGLKVERYDGQSYVIQWEAAIAADPDWVLITSWNEWHEGSEIEPSL
                     EHGRKYLELTRQMTARFAPSART"
     gene            complement(1302..2900)
                     /gene="crtI"
                     /locus_tag="PKI05_09090"
     CDS             complement(1302..2900)
                     /gene="crtI"
                     /locus_tag="PKI05_09090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HOA52401.1"
                     /translation="MEVNLWLTTFLAGRRSKLGDPYGLHQVVIIGAGPGGLGAALLLA
                     KAGLDVTVLERLSVIGGRCSPITINGYRFDRGPTFLHYPCALKSIFRAVGKELEEEVP
                     MVRLDPMYRLFLGERGYLDATADIAQMEAHIAKFQPRDCKGFEQFLKDNDGKFQRVIR
                     TLQRPFDGLRDLFSSEILNLLPVLRPFTSLQQDMRKFFSDPDLQVAFTFQSKYLGMAP
                     DRCPSMFSILSYLEYAYGIFHPIGGCNAVPQKLAKLSKELGAKFHLNQEVQEIRFEGR
                     RPVAVKTQDAEFPCDLLVINADFARAMERLVPNVLRRRWSDEKLRSKKYSCSTFMLYL
                     GIEGQVENLLHHNIYIPPHYREYLDDVDVRHVLSDAPAFYVQNATLTDPTLAPPGHST
                     LYILVPVTHQHPNVNWDQERARYREVVLDNLTRIGIPDLRSRIRAELVWTPADWEAWQ
                     IYRGATFNLAHSLDQMLIFRPHNRFEELERTYLVGGGTHPGSGLPTIFESARISAKLI
                     LEDLGRDARFLAPGPMEPPLIQLT"
BASE COUNT          707 a          796 c          865 g          650 t
ORIGIN      
        1 catcgtcctt ccggtacctg cggaacgcgt caaggtctat gccctggacg aaacgggtcg
       61 gcgcaagggg agcgtgccga tacaaccctc gggtcaggcg gcgtttgaaa ttggccccaa
      121 gtatcaaacg ttgtggtacg aggtggaagt ccaaccgtga cccattctga gcaacatctg
      181 aagatgatca gccgacaaga ggagccttac tatgatcacg cgccgtggat ttcttagaca
      241 gaccagcgtg ttggggacag cgagcctggt accgaacttg cttaccaccg gctggggtct
      301 tgaacctaac cacgtcccgc tttcccgtga aaaggtcgga cgccatattt tggcattcta
      361 ctatccctgg tatggaaatc ccgccgcttc gggtggaagt ggtcgctgga gtcactggca
      421 agacgtcgat gaacaagcca agacgatcgg ttctagcact cactatccgg agctgggacc
      481 ttatgattcg catgaccgca aggtcattac gcagcactgc cgctgggcgg tcgaggctgg
      541 tctcacggga tggattgcga gttggtgggg acacaggtct ttcgagaatc aggccttgcc
      601 acgaattctc gatatctcgg gcgaacacaa actggccgtg acgatctact acgaaacgat
      661 cccaggacag cccaagaccg tggataatgc cgtgaccgac atcgttcgct tgttggagca
      721 acatgccaac catccggcct ggctgacggt ggacggcaaa cccgtcattt tcatttatgg
      781 ccgggccgta ggagaaattg gtgtagccgc gtggggtgaa gttatccaaa aggtcaatgc
      841 cgcttatccc cgcggggcca ttttccaggg tgatcagttt tctgcggcag cagccaagat
      901 gtttgacggc cttcacactt acaatacggc gggtcagctg cgaggtaagt cgctggagga
      961 ggtcaaatcg tggtgtgccg agaggtatcc ccaatgggtg aagctcgccc gcggtgcagg
     1021 gaagatcagt agcctgaccg tcattcccgg ctacgatgac accaagatcc gcaaacccgg
     1081 cttaaaggta gaacgttacg acggccaaag ttatgtcatc cagtgggagg cggccattgc
     1141 ggccgatccc gattgggtct tgatcacctc gtggaacgaa tggcacgagg ggagtgaaat
     1201 tgaaccgtcc ttggaacacg gcagaaagta tcttgagtta acacgacaga tgaccgctcg
     1261 atttgctccg tcggcgagaa cctagcgcgc cgtccgcatg gctaggtcaa ctgaatcagt
     1321 ggtggttcca tgggcccagg ggcgaggaat ctggcgtcgc ggccaaggtc ttccaggatg
     1381 agtttggccg agatcctcgc cgactcgaaa atagtgggca aaccgctgcc ggggtgggtt
     1441 ccgcctccga caagataggt tctctccagt tcctcaaatc gattatgcgg gcggaagatg
     1501 agcatttgat caagtgaatg cgcgagattg aatgtggcac cacggtagat ttgccacgcc
     1561 tcccaatctg ccggtgtcca gaccaactca gcacggatgc gagatcgcag atcggggatg
     1621 cctattcgtg tcagattatc aagcacaacc tctcgatagc gtgctcgttc ttgatcccag
     1681 ttgacgttgg gatgctgatg cgtaacagga accagaatat acagggtgct atgtcccggc
     1741 ggcgctaacg tgggatctgt caacgtggca ttttgtacgt agaatgccgg cgcatcggag
     1801 aggacgtgcc tcacgtccac gtcatcaagg tattcgcgat agtggggagg gatgtagatg
     1861 ttgtgatgaa ggaggttctc cacctggccc tcgattccca ggtagagcat gaatgtcgag
     1921 caggagtact tctttgatcg cagcttttca tcagaccaac gtcgccgcag aacgttgggc
     1981 acgagccgtt ccatggcccg ggcaaaatct gcgttaatca ccaagagatc acacggaaat
     2041 tctgcgtcct gcgttttgac ggccaccggc ctgcggccct cgaagcgaat ttcctgcacc
     2101 tcctggttga ggtggaactt tgcaccgagc tctttcgata acttcgcaag cttctgggga
     2161 acggcattgc atccaccgat cgggtgaaaa atgccgtacg catattcgag ataagaaagg
     2221 atcgaaaaca tgctggggca gcgatcaggt gccattccca gatatttgga ttgaaatgta
     2281 aaggcaacct gtaaatccgg atcgctgaaa aactttctca tgtcctgctg caaggaagtg
     2341 aacggtcgca aaaccggcaa gagattcaga atctcggaag agaaaagatc tctcaaaccg
     2401 tcaaaggggc gctggagcgt gcgaatgacc cgctggaatt tgccgtcgtt gtctttgagg
     2461 aactgctcga atcctttgca atcccgcggt tgaaacttgg cgatatgggc ttccatctgc
     2521 gctatgtccg cggtggcatc gaggtatcct cgttccccta ggaaaaggcg gtacatggga
     2581 tcgagccgga ccatgggaac ctcttcttcc agttcttttc ccacggcccg aaagatgctc
     2641 ttgagtgcac acggataatg gagaaacgtt ggcccacgat cgaaacgata accattgatg
     2701 gtaatcgggg aacaccgccc accaatgaca ctgagccgct cgagtaccgt cacatccaaa
     2761 ccggccttgg ccagcagaag ggccgctccc aaacctccgg gaccagcgcc gatgatgaca
     2821 acctggtgca atccataggg gtctcccaat ttggatcgtc tgccggccag aaaggttgta
     2881 agccataaat taacttccat gacccacgcc ggcgtgctgc taatacttta catatccttg
     2941 aaggcatcca caattgccgc gttggatgaa accacggaat gggcttcgcc tcatcgggcc
     3001 agcagggttg ccttttga
//
LOCUS       DAOPTR010000445         3018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_26074_length_3018_cov_2.484306, whole genome shotgun sequence.
ACCESSION   DAOPTR010000445 DAOPTR010000000
VERSION     DAOPTR010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26074_length_3018_cov_2.484306"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1784
                     /locus_tag="PKI05_09095"
     CDS             <1..1784
                     /locus_tag="PKI05_09095"
                     /inference="COORDINATES: protein motif:HMM:NF016563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HOA52402.1"
                     /translation="SLQEVTTATSREGETVNGAVFSCVDIPPGETREVTYVLVWYLPG
                     QPAGTGAWGCKGRRYEAWWKNAQEVAAELEQRLPELEAASRMYVDSLYESNLPYWLID
                     RIASQVAILRSPTVFWCREGYFGGWEGCNVNEGCCHGNCNHVWHYAQAHARLFPEIAK
                     QMREQEFTYQKPDGAIPHRQPDSFPAFDGQCGAVLNSYREHLMSSDRSWLDSHWPAIR
                     RAMDYVIQRWDPDQDGLLSGPQWNTLDGELGGNSSWLGSLYLAALDAAEKMARLEGDN
                     TSAKKYQRIARLGAAKQDELLFNGQYYIQISDPTPHEDYGEGCAIDQVLGQWWAHQLD
                     LGWVYPPEHVTSALRTLFALNFRGRMEGLPQLPRKFVADEDGGMQMFVWPEGAKKPSQ
                     VIKYASEVMTGFEYAAAAAMIQAGLLKEGLTVCRAIALRYDGRKRVFPGAGNFNNWGY
                     SGNPFGDDECGKFYARAMSVWSILLACQGLIYDGPDQRIGFTPVWQPEDHVSFFTAAE
                     GWGVYRQSRGDGEQKHSLSVRFGQLAVKTFEGATPAGTTPKTVQCVLEKREIPCVLRC
                     HNGRFSCVLEGPVVVRQGQSLDIRITW"
     gene            1835..>3018
                     /locus_tag="PKI05_09100"
     CDS             1835..>3018
                     /locus_tag="PKI05_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52403.1"
                     /translation="MWTRQYPDNRRQICHLFGIWQGMLIVLVTFGITTVAVGESAGDG
                     QVVFAVIRGQQEGKNLLAEGTWGKYEQGFEEHEGVIVCENKDKPGYRAGAVQSIVLNQ
                     KEPRPLVAVAESAAQDVDGARDNNYSVYLDIEYNDGTPLWGQAAAFDVGTHDWQERRV
                     VIFPEKPIRRLSYYLLFRGHRGKAMFRNPRLYEISTPGTALFDGIPCTKAVGDYRGLV
                     VRDVAKNSDFVALQQSALGIECVWTVTEKDNVRTYDLILRSRSDEDRALTVAFVEPVK
                     CTSSGDKPILWLQSPRRSEAVSATREYATTVRTRAGATQRLGRWPLAAVATDTQGYGV
                     GIDMIYPAVFRVGYNAAYEQLFVAYDIALTKEKPDAHLRFVVFTFSPHGGFRAAWARY
                     IE"
BASE COUNT          679 a          736 c          918 g          685 t
ORIGIN      
        1 agtcactcca ggaggtaaca actgcgacct cgcgggaagg ggagacggtt aatggtgcgg
       61 tgtttagttg cgtggatatt cccccggggg agacgcgtga agtgacgtat gtcttagtgt
      121 ggtatttacc cggccaacct gccggcaccg gggcctgggg gtgcaaaggg cggcgctatg
      181 aggcttggtg gaagaatgcc caggaggtag cagccgagtt agaacagcgc ctgcctgaac
      241 ttgaggcagc atcgagaatg tatgtggact ctctttatga atcaaatcta ccctattggc
      301 ttatcgaccg gattgcctcc caagtggcca tcttgcggtc accgactgtc ttttggtgcc
      361 gagaaggtta tttcggtggg tgggaaggtt gcaacgttaa cgaaggatgc tgtcacggta
      421 attgcaatca cgtctggcat tacgcccagg cccatgcccg attgttcccc gaaatcgcaa
      481 aacaaatgcg ggagcaggaa ttcacctatc agaagccgga cggggcgatc ccccatcggc
      541 agccagattc tttccccgcg tttgatgggc aatgcggtgc cgtgcttaac tcgtatcgag
      601 aacatctgat gtcgtcggac aggagttggc tagactcaca ctggcctgcg attcgtaggg
      661 ccatggacta cgtgattcaa cgctgggacc cggatcagga cggactgttg tcagggccac
      721 aatggaacac acttgacggc gagttaggcg ggaattcatc ctggctggga agtctctatc
      781 tggcggctct tgacgctgcc gaaaaaatgg cccgacttga gggtgacaac acgtctgcca
      841 agaagtacca gcggatagcc agattaggcg cggccaaaca agatgaacta ctctttaatg
      901 ggcaatacta cattcagatt tccgatccaa cgccccacga ggactacggg gaaggatgtg
      961 ccatcgacca ggtgcttgga caatggtggg ctcatcagct tgacttgggc tgggtttatc
     1021 cgccagagca tgtgacgtcg gcgctaagga ctctcttcgc gctcaatttt cgaggccgca
     1081 tggaaggttt gccccaattg ccgcgaaaat tcgtggctga cgaggacggg gggatgcaga
     1141 tgttcgtctg gccagagggg gccaaaaaac cgagtcaagt cattaagtat gccagtgagg
     1201 tcatgacggg ttttgagtac gcggcggctg ccgctatgat ccaggcggga ttgctcaaag
     1261 aagggctgac ggtctgtcgc gcgattgccc ttcgttacga tggtcggaaa cgggtcttcc
     1321 ctggtgcagg caattttaac aattgggggt attcgggcaa tccctttggt gatgacgagt
     1381 gtggcaagtt ctatgcccgg gcgatgagcg tgtggtcaat cctcctggcg tgtcaaggtt
     1441 tgatttacga tgggccagat caacggattg ggtttactcc ggtctggcag ccggaagacc
     1501 acgtatcatt tttcacggcc gcagagggtt ggggggttta tcgccaatcg cggggtgatg
     1561 gtgagcagaa gcatagcctg agtgttcgct tcggccaact cgccgtcaag acatttgagg
     1621 gggcgacacc tgccggaacc acaccgaaaa cagttcagtg tgtcttggaa aaacgcgaaa
     1681 tcccatgtgt gctgcggtgt cacaatggcc gattttcctg cgttcttgag ggacccgtcg
     1741 ttgtccggca gggacaaagc ttagatattc gtattacttg gtaggaatgc aacctttgta
     1801 ccttcaatat gccagtggac gaaaggacat agagatgtgg accagacaat atcctgataa
     1861 ccgacggcaa atatgtcatt tgttcggaat ttggcagggt atgctgatag ttttagtgac
     1921 ctttggaata accaccgtcg ccgtgggcga aagtgctggc gatgggcaag tcgtttttgc
     1981 agtgattcgg ggccagcagg aagggaagaa tctcctcgcc gaaggaactt ggggaaaata
     2041 cgaacaagga tttgaagagc acgagggtgt tatcgtctgc gaaaacaagg ataagccggg
     2101 atatcgtgcc ggggcggttc agtcgattgt cttgaaccaa aaagaaccac ggcctctggt
     2161 cgccgtggcc gaaagtgcgg cccaggacgt ggacggtgcc cgagataaca attactccgt
     2221 atatcttgat atcgagtaca acgacggaac acccctttgg ggccaggcgg cggcgtttga
     2281 tgtgggcacc cacgactggc aagagcgaag ggtcgtcatc tttccggaaa agcccatccg
     2341 acgattgtcc tattatttgc ttttccgcgg gcatcggggt aaagcgatgt ttcgaaatcc
     2401 gcgtttgtac gaaataagca cgccgggaac tgccctcttt gacggaattc cctgcaccaa
     2461 ggccgttggg gattaccggg ggttggtcgt tcgtgatgtg gcgaaaaact cagactttgt
     2521 ggcccttcaa cagtcggcct tggggattga gtgcgtctgg accgtcaccg aaaaagataa
     2581 cgtcagaacc tatgacctta tcctgcggag tcgatctgac gaagatcgag ccctaacggt
     2641 tgcctttgtc gagccggtca aatgcacgtc gtccggtgac aaaccaattc tttggcttca
     2701 aagcccccgc cgcagcgagg ctgtttcggc aacccgcgag tacgcgacga ccgtccgaac
     2761 acgcgcgggg gctactcagc ggctgggacg atggccccta gcagccgtgg caactgacac
     2821 ccaggggtac ggtgttggga ttgacatgat ttatccagcg gtcttccgcg tgggctataa
     2881 cgccgcttac gaacaactat tcgttgcgta cgatatcgct ctaaccaaag agaagccaga
     2941 tgcgcatttg cgcttcgtgg ttttcacttt ttctccgcat ggaggctttc gggcggcttg
     3001 ggctcgctat atagagat
//
LOCUS       DAOPTR010000446         3016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_26093_length_3016_cov_3.444107, whole genome shotgun sequence.
ACCESSION   DAOPTR010000446 DAOPTR010000000
VERSION     DAOPTR010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26093_length_3016_cov_3.444107"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(321..1337)
                     /locus_tag="PKI05_09105"
     CDS             complement(321..1337)
                     /locus_tag="PKI05_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52404.1"
                     /translation="MFECKIRVRVVLLCLVTLQGVISWLTIHADEKTQSDNQLLEQVC
                     ACLLDLEKSYHRIKITGVERYEAGPVGEYVESLVTFWNRDAMRRLDREIIGPVNDRRI
                     GTVQAAVIKPRLAFEVQKAPKAENYVVTAMGPTPIPFELEADFRWYGKFILAPVSVGE
                     FALSKLLHSPDVLLADVSWREFDNSKCLVLDLRRDAPQGFVKGEVIVDPKLSFAVRRW
                     ELEMRGGYRGVCELEYTTRFGQIPIVEWGIIEWKKASDGSVVERYEWALKDVAFDELS
                     DEVFSMHAFGLPESRPIYRLPWLWAVHVILFAALVSALILVRYFRRRQWVRDEADEGP
                     ASSI"
     gene            complement(1735..2694)
                     /locus_tag="PKI05_09110"
     CDS             complement(1735..2694)
                     /locus_tag="PKI05_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HOA52405.1"
                     /translation="MANREQLTFASARFDVYRRPTRRDRFLQQMGKAMPWDSLIGLIA
                     PFYPKEGQPGRPVTPLVLMIKIYFLQIWFNLSDPEAEELMHDSLAVQRFLNLDLGRDR
                     PPDETTINRFRNLIKEHKLGEKILKTVNEHLERAGITIKKGTIVDATVISAPTSTKNE
                     RQSRDPEMGSTKKGDQWYFGAKLHVGVDSRTKVIHSVSLTPANVHDSQMLSELLHGGE
                     RRVYGDKAYVGQKEAIRKKAPRARRNAPLGEKELRSNRWKSRIRSRVEHVFGVIKHIF
                     GWVKVRYRGIEKNLQYAYGVAAAVNVYIHRRVLSEKTAALSPA"
     gene            complement(2757..>3016)
                     /locus_tag="PKI05_09115"
     CDS             complement(2757..>3016)
                     /locus_tag="PKI05_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52406.1"
                     /translation="TSPRAVALPNADRLATEQVFGADARVGHLSRDGTAEPIVTAAEE
                     SGSQQQGADSEDSSAWWWLNPWSYPGPRHIIKEPLHNSHVL"
BASE COUNT          704 a          940 c          663 g          709 t
ORIGIN      
        1 ttgtgggtgt gtcgtgtcaa cccccacgaa agtgtcgttt taagtcaccc cgtgggctga
       61 tgtgtacaat tgtaccgacc ccggcacctc agatgctccc cacaatcagt ctccaggtat
      121 ttcgccttac gctgtttcgg ataagagacc tccaaacaac cgccacgtac taagcgattc
      181 tgaatccgaa caaaggaatc ttcgcgctgt tgaagcggga actcattatg atacgccgca
      241 agtcctggtt cactgagaac tgggtatgtc ttacgtctgg aatctctgct agtgggcaag
      301 cattcactcc ggcttcccta ttagatagaa ctggctggtc cttcgtcagc ctcatcccgc
      361 acccactgac ggcgccgaaa atatcggacc aaaatcaagg cacttacaag cgccgcgaac
      421 aagatcacgt gaacggccca gagccaaggg agcctataaa ttggtctaga ctcaggcaat
      481 ccaaacgcat gcatgctgaa tacctcatcc gacaattcgt caaatgcgac atctttgagt
      541 gcccattcgt accgctcgac gacactgcca tccgaggcct ttttccattc gattatcccc
      601 cactccacaa ttgggatttg accgaatcgg gtggtgtact ccagctcaca caccccacga
      661 tagccgcctc gcatttcaag ctcccagcga cgcactgcaa aacttaactt cgggtcaacg
      721 ataacctctc cttttacgaa accctgaggt gcgtctcgac gcaagtccaa tacaagacat
      781 tttgaattat caaactccct ccaactgaca tctgccaaca gtacatccgg actgtgtaac
      841 agttttgata gcgcaaattc gccgacagag acgggagcca gtataaactt gccataccaa
      901 cgaaaatcgg cctccagctc gaatgggatc ggtgttggac ccatcgcggt cacgacatag
      961 ttttccgcct ttggagcctt ttgcacctcg aatgcaaggc gcggtttgat cacggcggcc
     1021 tgcaccgtgc cgattcgtct gtcattcacc ggtccaatga tttctcggtc aagccgtcgc
     1081 atggcgtccc gattccaaaa agtcacaaga gactccacat attctcccac tggaccggct
     1141 tcgtaccgct caacacccgt gattttgatg cgatggtaac ttttttccag atccaagagg
     1201 catgcgcaaa cctgttccag tagctggttg tcactctgcg ttttttcgtc ggcgtgtata
     1261 gtcagccaac ttattacccc ttgcagcgtg acaaggcata acaacacaac tcttacccga
     1321 atcttacatt cgaacatagt tgtccaccac ctggccacca agcccgccat ttaggttttc
     1381 agcatcccga aagatacaag acatacacgg actatcgtcc agtgatgcgc tcgttacctg
     1441 acgctcaata aataaggtcg cacgaagtcc ccccagcacg taaggtacag atacagttca
     1501 caggacagtt ctccccattg gcagtgtcgc accgggtgcc aggttcacat gaccagttac
     1561 acccggtaca tgatttagga acctctgcac aattaccaaa taggttgcga atatctcgat
     1621 ttgccattgt gcgtgatact ggaggattag attctattac cagataactt tctatcttgc
     1681 ccagttggcc tgatttatcg cctccggcca atctgggcgc gcaaggggag cgccctaagc
     1741 cggggaaagt gctgccgtct tttcactcaa gaccctccgg tggatgtaaa catttacggc
     1801 cgctgctacc ccataggcat actgaagatt cttctctatc ccccgatacc ggaccttcac
     1861 ccatccaaag atgtgcttga tcaccccaaa cacgtgctcc acccgactcc gtatccgact
     1921 cttccagcga ttgctccgca actccttctc ccccagagga gcgttccgcc tcgcccgcgg
     1981 ggccttcttc cgaatcgcct ccttttgccc cacatacgcc ttgtccccat acacccgccg
     2041 ctctccccca tgcaaaagct cggacaacat ctgactatca tgcacattcg ccggcgtcaa
     2101 actcaccgaa tggatcacct tcgtccgact atccactccc acgtgcaatt tggccccaaa
     2161 gtaccactgg tcccccttct tcgtgctccc catctccggg tcacgactct gcctctcgtt
     2221 cttcgtcgag gtcggggctg agatcactgt ggcgtccaca atagtaccct tcttaatcgt
     2281 gatccctgct cgctccaaat gctcgttgac agttttcaga atcttctcgc ccaacttgtg
     2341 ctccttgatc aaattccgaa accggttgat cgtggtctca tccggtggac gatcacgccc
     2401 caggtccagg ttcaaaaaac gctgcacagc caacgaatcg tgcatcaact cctcggcctc
     2461 ggggtcagaa aggttaaacc atatctggag aaagtagatc ttgatcatga gtaccaaagg
     2521 cgtcaccggt cgtcccggct gcccttcctt gggatagaac ggcgcgatga gaccgataag
     2581 actgtcccac ggcatcgctt ttcccatttg ctgcaaaaac cggtcacgcc gggttggacg
     2641 acgatagaca tcaaaccttg ccgaggcaaa agtcagttgc tcgcgattgg ccatctgggc
     2701 aattccccgc aattaaggca tcctccgtcc ttaccttcgc aaaaaactcc actccattat
     2761 aatacgtggg agttatgcag aggttcctta ataatgtgcc gaggacctgg atatgaccaa
     2821 ggattgagcc accaccacgc gctgctgtcc tccgaatccg cgccctgttg ctgtgaacca
     2881 gattcctccg cggccgtcac gatcggttca gccgtaccgt ccctggagag atggcctacc
     2941 cttgcatccg ccccaaaaac ctgctcggtt gcaaggcggt ccgcgttggg taaggcaacc
     3001 gcccgtggac ttgtag
//
LOCUS       DAOPTR010000447         3008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_26191_length_3008_cov_3.333898, whole genome shotgun sequence.
ACCESSION   DAOPTR010000447 DAOPTR010000000
VERSION     DAOPTR010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26191_length_3008_cov_3.333898"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..646
                     /locus_tag="PKI05_09120"
     CDS             107..646
                     /locus_tag="PKI05_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2617 family protein"
                     /protein_id="HOA52407.1"
                     /translation="MLSVRPRVTELAFVLYGRPLHPELFEIRATRHIARGDYQARLDI
                     TNTGHIITWNYEGLTWTEVATAAHHPLPAKRRLMFHNIKGELRDEVIGRGGVRYEWEA
                     QLEHVSPEVFWVFQHELNREAPQQGLFFRFDPSGRMPLGAMSFLYFETRAKSLLVQAF
                     HTFPDDCAVVKSKTVIRLP"
     gene            complement(724..1539)
                     /locus_tag="PKI05_09125"
     CDS             complement(724..1539)
                     /locus_tag="PKI05_09125"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOA52408.1"
                     /translation="MRIVSLPDKLSEGNTFSMAVEDQGQGRTILLVHAFPFDHRVWEA
                     QIKGLSTRFRVLAPDLRGFGRSTVTPGVVTMEQMADDLAAMLDALAIDEPVVLCGLSM
                     GGYVAFQFVRRHRQRLRGLILCDTRAASDSQEARENRLRTAERVITEGTAFLADQMLP
                     RLCCERTFRHQPEIVTKLRTMILEAPPEGVAAAARGMAQRPDSTPLLGQIDCPTMVVV
                     GQFDTISLPEEMEAMARAIRGASFAVVPDAGHMAPLEQGEHTTQLIAEFASRL"
     gene            complement(1715..>3008)
                     /locus_tag="PKI05_09130"
     CDS             complement(1715..>3008)
                     /locus_tag="PKI05_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen debranching enzyme"
                     /protein_id="HOA52409.1"
                     /translation="TLSFKGLENRVYYMLENGGSRYKNYTGCGNTLNGNHPIVREMIF
                     HCLRYWVHNYHIDGFRFDLASILSRDRAGNLVPNPPVVETIAEDPLLADTKIIAEAWD
                     AAGAYQVGTFASLCWPDTFAHCRWAEWNGRYRDDVRRFWRGDPGMVPAFATRIAGSSD
                     LYQPGGRYPYHSINFITSHDGFTLNDLVSYNHKHNEENGEDNRDGENNNNSYNYGVEG
                     PTWHKGINLIRLRQIKNMMATLLLSQGVPMILFGDECRRTQRGNNNAYCQDNSISWFN
                     WKLVHRHRELWRFVQALIAFRRREPTVRRKDFLTGLPVRPGGFPDVSWFNPAGGPVDW
                     TNAGRSLTCFLAAFPHREDPNRPNHHILLMFHAAPEPTRFVIPAMARGLAWRLFIDTS
                     RMSPNDIYPDLDGPPPPVDWTLNVPGRTLICYVAPDLL"
BASE COUNT          680 a          826 c          834 g          668 t
ORIGIN      
        1 atcaggcaat tagcaaagtg acagcggcgc cttgtcgcag gccttcgtga cacagtgggt
       61 catatttccg agagtgagtc tcggcttaag aaggaagggt acagccgtgc tgagtgttcg
      121 tccgcgtgtt accgaattag cctttgtcct gtatggacgg ccgctccacc cggagctttt
      181 cgaaatccga gccacgcggc acattgcgcg cggggattat caggcccgac tcgacatcac
      241 caacacgggc cacattatca cttggaatta tgaagggctc acgtggactg aggttgccac
      301 tgccgcccat catccgttgc ccgcaaagcg gcggcttatg tttcataaca tcaaagggga
      361 gcttcgggac gaggtaatag gacgcggtgg agtccgttat gagtgggagg cccaactcga
      421 gcacgtctcg cccgaggttt tctgggtctt tcagcatgaa ctcaatcggg aagcgcctca
      481 gcaaggcctt ttcttccgct ttgatccaag cgggcgcatg cccttggggg cgatgagctt
      541 tctttacttt gaaacgcggg ccaagagtct cttggtgcag gcgtttcaca catttcccga
      601 tgactgtgcc gtggtcaaaa gcaagactgt aattcggcta ccttgaagag tctcggcgtt
      661 tcgatctgct cagagcaccg cagctaatta gcttgttcaa aacaattgcg acgttgatcg
      721 tctctacaat cgcgaggcaa attcggcgat aagctgcgtc gtatgctcgc cctgttcaag
      781 aggggccatg tgacctgcat cgggcacaac agcgaatgat gccccccgaa tggccctagc
      841 cattgcctcc atttcttctg gcagggagat cgtatcaaat tgccccacaa ccaccatggt
      901 cggacaatcg atctgaccca aaagcggcgt ggagtcagga cgctgcgcca ttcctcgtgc
      961 tgccgcagcc actccctctg gtggtgcttc taggatcatc gtgcggagtt tcgtcacaat
     1021 ctcaggctga tgacgaaagg tccgctcgca acacaggcga ggaagcattt ggtcggcgag
     1081 gaaggcggtt ccctcggtga taactcgttc cgccgtacgc aaccgatttt ctcgagcttc
     1141 ttgggagtct gacgcagctc gcgtgtcgca cagaatgagt ccgcgaagcc gctgtcgatg
     1201 acgtcgcaca aattggaaag ctacgtagcc tcccatcgac agaccgcaaa gcacaaccgg
     1261 ttcatcaatg gccagggcgt cgagcattgc cgcgaggtca tccgccatct gttccattgt
     1321 gaccacaccc ggcgtcaccg tacttcggcc aaatccccgc aagtccggag cgagcacccg
     1381 aaagcgtgtg gataagccct tgatttgggc ctcccaaacc cgatggtcga aaggaaaagc
     1441 gtggaccaag agaatcgtcc gaccttggcc ttggtcttcc acggccatgg aaaacgtgtt
     1501 cccttcggaa agtttgtccg gcaagcttac gattcgcata gttgtttacc cctaaagagg
     1561 gtgaacccat gcactcactg cttcgggcac gcaatcaaaa acggtccctc tagaccgcac
     1621 gtctggacca cacgatgggc agtttacctt tgacgggcag agtatttagc ccgcattgtt
     1681 attttatggc ccggtctttc gataacataa atcattacaa aagatcgggc gcaacatagc
     1741 agattaacgt acggccggga acattgaggg tccaatcaac aggcggcggc ggaccgtcga
     1801 gatcgggata gatgtcgttg ggcgacatgc ggctggtgtc gatgaacaag cgccacgcta
     1861 gaccccgcgc catggccgga ataacaaatc gcgtgggttc cggagctgcg tggaacatca
     1921 gcaagatatg atggttgggg cgattgggat cttcgcggtg ggggaacgcc gctaggaaac
     1981 atgtgaggct tcgtcccgca ttggtccaat cgacaggccc gcctgcgggg ttaaaccaac
     2041 tgacatcggg aaatcccccg gggcggacag gaagaccggt gaggaagtcc ttgcgtcgca
     2101 ccgttggttc ccgcctgcgg aaggcgatca aggcctgaac aaaccgccac aattcccggt
     2161 ggcgatggac caatttccaa ttgaaccagg aaatagaatt gtcctggcaa taggcgttgt
     2221 tgttgccccg ttgggtacgt cggcactcat cgccaaagag aatcatgggc accccttggc
     2281 ttaataacaa tgtagccatc atattcttga tttgccgcaa tcggatcaaa ttgatccctt
     2341 tgtgccaagt gggaccttca acgccataat tgtaactgtt attgttgttt tcgccgtctc
     2401 ggttatcctc accattctcc tcgttgtgtt tgtggttata gctgaccaga tcattgagcg
     2461 taaaaccatc gtgggaagta ataaaattga tgctgtgata cggatagcgg ccacctggct
     2521 gatacaaatc gctggaaccc gcgatccgtg tcgcgaacgc cggaaccatc ccggggtcac
     2581 ctcgccagaa tcgccgaacg tcgtcccgat agcggccgtt ccattctgcc catcggcaat
     2641 gggcaaacgt atcgggccag cagagggatg cgaacgtccc cacctgatac gcccccgcag
     2701 catcccaggc ttcggcaata atcttggtgt cggccagtag cggatcctcg gcgattgtct
     2761 caaccacagg aggattgggg acaagattcc ccgcccgatc gcggctgagg attgatgcca
     2821 ggtcgaaacg gaaaccatca atgtgataat tgtggaccca ataacgcaag cagtggaaga
     2881 tcatctcgcg gacgatgggg tggtttccgt taagcgtgtt tccgcaaccg gtgtagtttt
     2941 tgtaacggct gccgccattt tctagcatgt aatagacgcg gttctccaat cctttgaagc
     3001 tcagtgtc
//
LOCUS       DAOPTR010000448         3002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_26260_length_3002_cov_4.386495, whole genome shotgun sequence.
ACCESSION   DAOPTR010000448 DAOPTR010000000
VERSION     DAOPTR010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26260_length_3002_cov_4.386495"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PKI05_09135"
     CDS             complement(<1..373)
                     /locus_tag="PKI05_09135"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52410.1"
                     /translation="MMNPFWKNDEQLFAVFRQELSTAIVGEVLDQMGLNAQFLPGCIR
                     PLDPRMVVVGRAMPVLFADCFETFSPSGLNPVFAKPFGLMLEALDDLRPMEVFVATGG
                     RSELAMWSELMTVRAVRLGAAG"
     gene            725..1636
                     /locus_tag="PKI05_09140"
     CDS             725..1636
                     /locus_tag="PKI05_09140"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HOA52411.1"
                     /translation="MSALILSTWHFGRIANVTGWPILENGGSALDAVERACAAVEEDP
                     EVTSVGYGGFPDASGEVTLDALIMVAPSRCGAVACLRGFIPAISVARRVMEKTSHVLL
                     VGPKAEEFAAAEGFTRRDLLTDRARRAWEEWKGRQNRQGQTAAEASVASTTADSRSNH
                     DTIGVLAQDAAGNLAGGCSTSGLAFKLPGRVGDSPIIGHGLYVDPDVGAAVGTGHGEL
                     IMRVCGTFLIVEEMRQGKLPKDAIRTALQRIADVCHPLPKQQAAFIALRKDGLWAAGA
                     LRPAFQVAIRSPTEDDLLAPQIVFWPE"
     gene            complement(1963..2661)
                     /locus_tag="PKI05_09145"
     CDS             complement(1963..2661)
                     /locus_tag="PKI05_09145"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOA52412.1"
                     /translation="MKTHLWLSVHALLVGLALAYAVKAEEGWITIPIEKGLPGWKLKG
                     QPEQSKWQVGTAAVDPNDPKSLICQPGGHELVNVGSRGVDIFTEQTFGDAIIELEVMV
                     PQGSNSGIYVMGEYEIQILDSYGKQTLGMGDMGAVYSMAVPKVNACKKPGEWQKYVIE
                     YRAPKFDATGTKIANGRLVKVILNDQVIHENLELKSQTPGGVTGKEAPRGPLMFQGNH
                     GPVAYRNIRIKPLN"
     gene            complement(2759..>3002)
                     /locus_tag="PKI05_09150"
     CDS             complement(2759..>3002)
                     /locus_tag="PKI05_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4976 domain-containing protein"
                     /protein_id="HOA52413.1"
                     /translation="HYHPGGATPYSAVRDGDWKLIEFFEDHHVELYNLKDDPQESQDL
                     ASQLPEKAESLRRQLHRWREEVGAQLPTPNPDYQPE"
BASE COUNT          621 a          896 c          856 g          629 t
ORIGIN      
        1 ccccggccgc gcccagtcgg acggcccgca ccgtcatcaa ttcactccac atggccagtt
       61 cgcttcggcc accggttgct acaaacacct ccatgggacg caaatcatcc agggcctcca
      121 gcatcaagcc aaagggttta gcaaaaacgg gattcaagcc gctgggcgaa aacgtctcaa
      181 aacagtcggc aaagaggacc ggcatcgccc gaccgaccac caccatccgc ggatcgagcg
      241 gccgaataca gcccggcaaa aactgcgcgt tgaggcccat ctggtcgagc acctcgccca
      301 cgatggccgt ggaaagctct tgccgaaaca ccgcaaagag ctgctcgtcg tttttccaga
      361 aaggattcat catggttgtc tccagaaaag atcctggcac acaccccgca actgcggcgc
      421 cacttgcgac caacgctcaa gagtgagagg gtagtcgcta acataagttt gcgcaaaata
      481 ttcagtttgt caattttccc caagcccgta atccggcaaa agccccaacc ttcggcccgc
      541 acaagtgcgc cacggctacc gcagcgcatg gcccagcaga atcgctacga atcgtcgttg
      601 gacactgccg ccaaaatgat cgcgaaaagc cccctgctgg cgtctggacg tagtgttggt
      661 cactaccgcg ttctgcgtca aaatagcata atcatctgaa accatccttc tgtgcgaggt
      721 gacaatgtcg gccttgattc tcagtacatg gcattttggt cggatcgcca acgtgacggg
      781 ctggcccatc ttggaaaacg gtggctctgc cctggatgcc gtagaaaggg cctgcgcggc
      841 ggttgaggaa gacccagaag tgaccagcgt cggttacggc ggcttcccgg atgcgtcggg
      901 cgaggtgacc ttagacgctc tcatcatggt ggccccttct cgctgtgggg cggtcgcctg
      961 tctgcggggg ttcattccgg cgatctccgt ggcccgacgt gtcatggaaa agacctcgca
     1021 cgtcctcctc gtcggcccta aggcggaaga attcgccgcc gcggaggggt tcacgcgtcg
     1081 agatttgctg accgatcggg caaggcgtgc ctgggaggaa tggaaggggc gacagaatcg
     1141 ccagggacaa accgccgccg aggcaagcgt tgcctcaacc accgcagatt cacgtagcaa
     1201 tcacgacacc atcggtgtct tggcacagga cgcggcgggg aacctggccg gggggtgctc
     1261 caccagtggc ttggccttca aactgccggg tcgggtgggc gactcgccca tcatcgggca
     1321 cgggctttat gtggatccgg acgtcggcgc cgccgtggga accggtcacg gcgaactgat
     1381 catgcgggtc tgcgggacat tccttatcgt ggaggaaatg cgccaaggaa agctgcccaa
     1441 ggacgccatc aggactgccc ttcagcgaat tgccgacgtt tgtcacccct tgcccaagca
     1501 gcaggcggct tttatcgcat tgcgaaaaga tggcctttgg gccgccggtg cgctgcggcc
     1561 ggcgtttcag gtcgccattc gcagtcccac ggaggacgat ctgttggccc cgcagatcgt
     1621 gttttggccg gagtgaacgg cgtttctcga agacttagag cgtatcccaa aacctcccct
     1681 cgcccgttcg ccgggagaga ggccgggggt gagggtggct cgaatcgctg ctggttttct
     1741 cgatcgactc cgacgatcgc ttgccctcac cctaaccctc tcccagaggg agagggaact
     1801 ttgggatggg ctctgaatca aagccccttc ggtctcgctc gccgaagggg ccttcttatt
     1861 gagtttgcta tcgaagcggc tcatcgaaac gatacaaaaa cagcgcgcca ccccgggcca
     1921 gcgtgatttc gtaaccgatt acttgtagcc ttcgattggt cttcaattta gcggcttaat
     1981 acggatgttt cgataagcca ccggtccgtg gttgccctgg aacatcaggg gcccacgggg
     2041 tgcctcttta ccggtgacac cgccgggcgt ttgtgacttg agttcaagat tctcgtggat
     2101 gacctgatcg ttgaggatga ccttcacgag acggccgttc gcaatttttg tacccgtggc
     2161 gtcgaacttg ggagcacggt actcgatgac gtacttttgc cattcccccg gctttttgca
     2221 ggcgttgacc tttgggacgg ccatgctata aaccgcgccc atatcgccca tgcccaaggt
     2281 ttgcttacca tagctgtcga ggatttgaat ctcgtattca cccatgacgt agattccgga
     2341 atttgatccc tggggaacca tgacctccag ctcgatgatc gcatctccga aggtctgctc
     2401 ggtgaagatg tccacaccgc gtgatccaac gtttacaagc tcatgcccgc cgggctgaca
     2461 gataagtgac ttgggatcat tggggtccac ggcggccgtc ccgacttgcc acttgctttg
     2521 ctccggttga cccttcagct tccaacccgg gagacccttt tcgatgggaa tcgtgatcca
     2581 gccttcttcc gcctttaccg cataggccag ggccaagccg acgagcaatg cgtgcacgct
     2641 caaccaaaga tgcgtcttca tgtgctcact cctttcataa aaggtacgaa caaactcgcg
     2701 aaagctcgaa atgtctccgt ccaactggga gacacgaggt tcattgcggc aggaagcgtt
     2761 attccggttg atagtcggga ttgggggtgg gtaattgggc accaacctcc tcacgccagc
     2821 gatgcaattg cctgcgaagt gactccgcct tctctggcaa ctggctggcc aagtcctggg
     2881 actcctgggg atcatccttc agattataaa gctccacgtg gtgatcttcg aaaaattcga
     2941 tgagtttcca atcgccatcc cgcacggcgc tgtagggcgt ggcaccgcca ggatgataat
     3001 gc
//
LOCUS       DAOPTR010000449         2993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_26357_length_2993_cov_4.146698, whole genome shotgun sequence.
ACCESSION   DAOPTR010000449 DAOPTR010000000
VERSION     DAOPTR010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26357_length_2993_cov_4.146698"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="PKI05_09155"
     CDS             <1..1287
                     /locus_tag="PKI05_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52414.1"
                     /translation="IELLVRLLESLFPEQADAFRDVIENLKNLWSAMWERETRRPVPE
                     ELLYPPVPTPPPRPETPLSPWERLINRIQEIWEKSVDWIRERWNKAITWVGERLEDGL
                     SFIADAKEWFGKPLEERTEDLKNAFATVALRIMDFAAAMPEILGQVATGIAIFSQTLV
                     GKVPGLAQAINTYRNLVETQNGIVAGTVAVLELLEANSGELASSLRGLMVPLKIVSAA
                     FGDALAPVLEALWPVFKALGIFILTVIEGVSTVWNALVGTIGGLFDAIANLEIFGRKP
                     FKSLQGIADFFNKLKVDTDALSKAKKELTELTFDQAKALKDATEKMREFGANVPTIFK
                     RALRVFQSSEGIPALASGGYVPATPGGRIVRLAEGGQGEYVIPESKMGWFGGPTIVVN
                     VDLGNAHIYGVDDLDDRIKRNVGEAIRDAAAVLTGA"
     gene            1303..1659
                     /locus_tag="PKI05_09160"
     CDS             1303..1659
                     /locus_tag="PKI05_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52415.1"
                     /translation="MSAIATFDGIPIHKINEIETSIKEMADVQRTPIGLERRDVAGPP
                     KRVWRVTTSPLHITLITPLEDHLWEIWWGYGEWWCWDLPKNTTVEARIDAGSWKKSTI
                     RGLYDHRILSFTVIER"
     gene            1913..2221
                     /locus_tag="PKI05_09165"
     CDS             1913..2221
                     /locus_tag="PKI05_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52416.1"
                     /translation="MDQRTDLIVRIKLQDENGDALKIEGASFLFAAGPINKTSVEGGG
                     IVIADEEGGVIEVVITHDDVQDAPAHYRYELLMTDVNGARYIPLKGLLIVTPSITARR
                     "
     gene            2226..>2588
                     /locus_tag="PKI05_09170"
     CDS             2226..>2588
                     /locus_tag="PKI05_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52417.1"
                     /translation="MADITLGQFYNAIAQGAREATLDALKGTDFATQTTLAAVLAKLS
                     DDPATESTLDDILSKLSSDPSTATLQTAIKNAVDAVANKVATETTLSAAKGVLDTIAG
                     KDFATQTTLAAVLAKLSDD"
     assembly_gap    2589..2688
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2689..>2993
                     /locus_tag="PKI05_09175"
     CDS             <2689..>2993
                     /locus_tag="PKI05_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52418.1"
                     /translation="GVLDTIAGKDFATQTTLAEILSKLSDPATQATLLDVLNKLDDLT
                     AVKNLLTEGDAKVQLKGSLPPSMMELAVNDWSELPDPTETPKGATAQLIGTFDVRQNT
                     "
BASE COUNT          709 a          722 c          831 g          631 t
ORIGIN      
        1 atcgaactac tcgtaaggct gcttgagagt ctgtttcctg agcaagctga tgcctttcgt
       61 gatgttatcg aaaacctgaa gaacctctgg tccgccatgt gggagagaga aactcggagg
      121 ccggtaccgg aagaattgct ttatccgccg gtacccactc caccacccag gcctgaaaca
      181 cccctcagcc catgggagag gcttatcaat cgcatccaag aaatctggga aaagtccgtc
      241 gattggatac gtgaacgttg gaacaaagcg ataacgtggg taggggaacg cctagaagac
      301 ggcctttctt ttattgctga tgccaaggag tggttcggaa agccgcttga agagcgtaca
      361 gaggacctca agaacgcatt cgccacagtc gctcttcgca tcatggactt tgccgcagca
      421 atgcccgaaa tcctcggcca agtggcgacc ggcatcgcta tctttagtca gaccctggtc
      481 ggaaaggtgc caggcttggc ccaggccatc aacacgtatc ggaacttagt ggaaacccaa
      541 aacggaatcg tggcgggcac tgtggctgtg ctcgaactcc tggaggcaaa ctctggggag
      601 ctggcgagtt ctctgcgggg gttgatggta ccgcttaaaa tagtttccgc ggcgtttggg
      661 gatgcccttg ctcccgtgct tgaggctctg tggcctgtgt tcaaggcact gggcatcttc
      721 attctgaccg tgatagaagg tgtcagtacg gtctggaatg ccttagttgg cacgattggt
      781 ggcctatttg atgcgattgc gaacctcgaa atcttcggcc ggaagccgtt caaatccctc
      841 caaggcatcg ctgacttctt taacaagctc aaggtcgata cggacgctct ctccaaggcc
      901 aaaaaggaac ttaccgaact cacgtttgac caagccaaag cgctcaaaga cgcgacagag
      961 aaaatgcgcg aattcggtgc gaacgttcct acgatcttca aacgcgccct gcgtgttttc
     1021 cagtcctcag aaggcatccc ggctcttgca tcgggcggtt atgtacccgc cactcccggt
     1081 ggacgcattg tacgcctcgc tgaaggcgga cagggcgaat acgtgatccc tgaaagcaag
     1141 atggggtggt tcggtggccc caccatcgtt gtgaacgtgg atctaggtaa cgcccatatt
     1201 tacggcgtgg atgacttgga tgaccgaatc aagcgcaacg ttggcgaagc aattcgtgat
     1261 gctgccgcag tgcttaccgg agcgtaaggc aggtggttac tggtgagtgc aattgccaca
     1321 ttcgatggca tcccgatcca caagatcaat gagatcgaga cttcgataaa agagatggcc
     1381 gatgtgcagc ggacgcccat tggattggaa cgtcgtgacg tcgctggtcc acccaaacgg
     1441 gtgtggcgcg tcacgacatc cccgctacac attactctca taaccccact agaagaccac
     1501 ctgtgggaga tctggtgggg ttatggtgag tggtggtgct gggaccttcc taaaaacacc
     1561 actgtggaag cccgaataga tgcgggtagt tggaagaagt caacgattcg ggggctttac
     1621 gaccatagaa ttctgtcttt caccgttatc gaacgttagg aggtgggcgc acgttgggta
     1681 ttgagtatcg acctagcgat acgtggcctg ctgaggcggg tcaagacgaa tccactccgc
     1741 taggcaatga atccaccgta ataggatatg aagatgacag tgcgttaaaa ctaatagaaa
     1801 tgctgacagg gcgcccgcca attaatcgaa tcgttcgggt gcgagaaatc acagtaacgc
     1861 taccaacgaa aatcgtaatt gaagcggtgg tgaggtagct gtggacattc gtatggacca
     1921 gcgaacggac ctcatcgttc gtatcaagct gcaagacgag aatggggacg cactcaagat
     1981 cgagggtgcg tccttcttat ttgcggctgg cccaatcaac aaaacgagtg tcgaaggcgg
     2041 cggcattgtg attgcggatg aggagggggg cgtaattgag gttgtcatta ctcatgacga
     2101 cgtgcaagac gccccggctc actaccgtta tgagctgcta atgactgatg tgaatggtgc
     2161 acgatacatc cccctaaagg gtttgcttat cgtgactccg agcatcacgg caaggaggta
     2221 gtgcaatggc cgacattacg cttggacagt tttacaacgc gattgcacaa ggtgcccgag
     2281 aggccacatt ggatgccctc aagggcacgg atttcgctac acagacgacg ttggcagctg
     2341 tgttggcgaa gctgtcggac gatcctgcta cagaaagcac gttggacgac attttgtcga
     2401 agttatcgag cgatccgtcc acggccacgc ttcagacggc gatcaagaat gctgtagatg
     2461 cagttgcgaa taaggtcgca accgagacaa cattatctgc cgctaagggc gtgcttgata
     2521 ccatcgcagg gaaggatttc gctacacaga cgacgttggc agctgtgttg gcgaagctgt
     2581 cggacgatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg cgtgcttgat
     2701 accatcgcag ggaaggattt cgctacacag acgacgttgg cagagatcct ttcgaagttg
     2761 tctgacccag ctactcaggc gactctgctt gatgtgttga ataagttgga cgaccttacg
     2821 gcagtcaaga atctgctcac agaaggcgac gcgaaagttc aactaaaggg cagtttaccg
     2881 ccgtcaatga tggaactggc cgtcaacgat tggtctgagc taccggaccc aacggagacc
     2941 ccaaaaggtg ccactgctca gctcattggt acgtttgacg tgcgccagaa cac
//
LOCUS       DAOPTR010000450         2993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_26366_length_2993_cov_2.733833, whole genome shotgun sequence.
ACCESSION   DAOPTR010000450 DAOPTR010000000
VERSION     DAOPTR010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26366_length_2993_cov_2.733833"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            254..913
                     /locus_tag="PKI05_09180"
     CDS             254..913
                     /locus_tag="PKI05_09180"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOA52419.1"
                     /translation="MSLLAYLAVGACLCGCRTGFLSVQKKARQELPPGIVLPREKIEK
                     WRELVNSAKDSGSAATATARLQEAFQGEDDPQLRAELVRLAGSLPPEAAWPIVELGLR
                     DENPDVQVQACRMVAKAKMAQAVGSLAKLAEESSNQDVRQAAIKALGQFKDPVAIQAL
                     AKALRDRDPAIQYLAIHSLRTVTGQDFGYDVAQWLAFLEGRQNPPQPTRAMATRPTQR
                     R"
     gene            1411..2700
                     /gene="eno"
                     /locus_tag="PKI05_09185"
     CDS             1411..2700
                     /gene="eno"
                     /locus_tag="PKI05_09185"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008695490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOA52420.1"
                     /translation="MSSTIVDILGRQILDSRGNPTVEVEVLLEDGSLGRAAVPSGAST
                     GTREALELRDGDPSKYLGKGVSQAVQNVNEKIAEEICGMDALDQAAIDYAMIELDGTE
                     NKSNLGANAILGVSLAVAHAAAQSSGLPLYRYLGGALARVVPAPMMNILNGGAHADND
                     VDIQEFMVMPLGFETFSDAIRCGCEIFHNLRKVLKDRKLSTNVGDEGGFAPDLKSNEE
                     ALECIKEAIDRAGYQLGEQVFLALDLAASELAVEGKKGMYMIGGKEVPAKAVIDMLES
                     WLEKYSICSIEDACSEDDWDGWALLTERLGKKVQIVGDDVFVTNTKIFAKGIESGIAN
                     SILIKVNQIGTLTETFECIDMARRAGYTYVISHRSGETEDTTIADIAVAAGGGQIKTG
                     SASRTDRIAKYNQLLRIEEMLGSTAIYGGKLFPKQWK"
     gene            complement(2786..>2993)
                     /locus_tag="PKI05_09190"
     CDS             complement(2786..>2993)
                     /locus_tag="PKI05_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52421.1"
                     /translation="LRPKIENENLFFCWICHNPSYNACTSPKLGRWTYARSYVWLQVT
                     ISGALMCKKQNKAPLAGTPRPILA"
BASE COUNT          693 a          715 c          907 g          678 t
ORIGIN      
        1 tcgaatcgtt gccggttttc tcgattaatt tcgacgttcg cttgccctca ccccagccct
       61 ctcccgggag agagggggtc tttggggtaa gctctaagtt acgcgccgga ccgcggcatg
      121 cgaggaatcc tgtttgatag cttcaatcgc ccctaaaaga tcgaacagcg acaatcggtc
      181 ccgaaaaagt accatgaaca cgcaaagatc agcgagccga actcatcacc aaactaaatg
      241 ccaaatcggt ttgatgtcgt tattggcata cctcgcggta ggggcgtgct tgtgcggctg
      301 tcggacaggt ttcctatcgg ttcagaagaa ggcccggcag gaactcccgc cgggtatcgt
      361 gctaccccgg gaaaagatag agaaatggag ggaactggtc aattcagcaa aggattcagg
      421 gtctgcggca acggcaacgg ctcgtttaca agaggccttc caaggtgaag atgaccccca
      481 gttaagggca gaactggtcc gattggccgg ttcgttgcca cctgaagccg cctggccgat
      541 tgtcgagctt ggactccgcg acgaaaaccc cgacgtccaa gttcaggcct gccgaatggt
      601 tgctaaggcg aagatggctc aggccgtggg ctccctcgcg aaactggcgg aggaatcaag
      661 taaccaggat gtccgccaag cggcgattaa ggccctcggg caattcaagg atccggtggc
      721 cattcaagcc ttagcaaagg ccctccgaga tcgtgacccc gccattcaat acctggccat
      781 tcactcgctt cggacggtca cggggcagga ttttggatac gacgttgccc aatggttggc
      841 gtttttggag ggccggcaaa acccgcccca acctactcgg gccatggcta cccgaccgac
      901 acagcgaagg tgatccggtt ttggggagat tgtgccagca ggatagtcct gaaatctggt
      961 ggcgtgctta caaaaagcaa cgcttttgaa tgtggatcgg ccacaaagaa atatcgcact
     1021 ggtcagttaa aattggcgat tcacgctctt tcgtgtagtc ccttccatga cctgagcaat
     1081 catctcgccg gacaatcatt aaagggctgt tttggtcatt cgacatagga aacggtgttg
     1141 gcactgattt tctcagggca aatcggtaaa ctgggtgttt ttggcttggt aggtcccttt
     1201 ttggcccacg tggttgtgga aatgtggtcc tagtcattgg cggatgaaaa gcgcaaaatg
     1261 gagggttatc gaccgccctg tggtcggtgg tcgcaaccat gccatctgga gtcttggatt
     1321 cagaccacgt ctttaggcct cggaccccga aggtgaaggg aacgtcggtt cacgattggt
     1381 agtacctgct ttcaagagag gaagtgaaac atgagttcaa cgattgttga catcctcgga
     1441 cgacaaatcc tagatagccg cggtaacccc acggtggaag tcgaagtgct tctggaagac
     1501 ggctcacttg gtcgggcagc cgttccgagt ggtgccagca cggggacgcg cgaagccctg
     1561 gagttgcggg atggcgatcc atcaaagtat ctcgggaagg gggtctcgca ggccgttcaa
     1621 aatgttaatg aaaaaattgc agaagagatt tgtggcatgg atgcgctcga tcaagccgcc
     1681 attgattatg cgatgatcga gctggatggc acagagaata agagcaatct cggggccaac
     1741 gccattcttg gtgtttcgtt ggccgtggct catgcggccg cgcagagcag cggcttgccc
     1801 ctgtatcgtt atcttggtgg cgcgcttgcc cgggttgtgc cggcacctat gatgaacatc
     1861 cttaatggtg gggcccatgc cgacaatgat gtggatatcc aagagttcat ggtcatgcct
     1921 cttggatttg agacattttc cgatgccatc cgctgcggtt gcgaaatctt ccataacctg
     1981 cgaaaggtcc ttaaggatcg aaaactgtcg acaaatgtgg gtgatgaggg tggctttgcg
     2041 ccggacctga aaagcaatga agaggccctg gagtgcatca aggaggcaat tgatcgggca
     2101 ggctaccaat tgggcgagca agtctttttg gccctagact tggctgcgag cgagttggcc
     2161 gtcgagggga agaagggcat gtacatgatc gggggcaaag aggtgccggc caaggccgtg
     2221 atcgacatgc tggaatcctg gctggagaag tattcgatct gctcgattga agacgcttgt
     2281 tctgaggacg attgggacgg ctgggccttg ctcacggagc ggctcggaaa gaaggtgcaa
     2341 atcgttggtg atgacgtctt cgtaacgaac acaaaaatct ttgcaaaggg tattgagtcg
     2401 ggtattgcca acagcattct gattaaggtt aaccagatcg gaacgctgac ggagacgttc
     2461 gagtgcatcg atatggcccg tcgcgccggt tatacgtatg tcatcagtca ccggagtggc
     2521 gagactgagg atactacgat cgcagacatc gcggtggcag cgggtggcgg gcagatcaag
     2581 acgggttcgg cttcgcggac tgaccgtatc gcaaagtata atcaactgtt gcggatcgaa
     2641 gagatgttag gttccactgc gatctacggt ggcaagctct ttccgaaaca gtggaaatag
     2701 cttacatgcg gcaaggtttt ccgcgaaacg ttcagggtgt tttccctgtc acgcagccat
     2761 cgtggtaagt gagggggcga aattgtcagg cgagaatcgg tcgaggcgtt cctgcgaggg
     2821 gcgccttgtt ttgttttttg cacataagtg cgccggagat ggtgacttgg agccagacgt
     2881 atgatctggc gtacgtccac cgaccgagtt ttggcgaggt acacgcattg tagctgggat
     2941 tatgacaaat ccaacagaag aaaagattct cgttctcgat tttgggtcgc aat
//
LOCUS       DAOPTR010000451         2991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_26383_length_2991_cov_3.038488, whole genome shotgun sequence.
ACCESSION   DAOPTR010000451 DAOPTR010000000
VERSION     DAOPTR010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26383_length_2991_cov_3.038488"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1562
                     /gene="priA"
                     /locus_tag="PKI05_09195"
     CDS             <1..1562
                     /gene="priA"
                     /locus_tag="PKI05_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOA52422.1"
                     /translation="KTILIHGVTGSGKTEVYIQAIEEVVRRGCQAIVLVPEISLTPQT
                     VERFRSRFHNVAVLHSHLTDAERAAQWEWIASGAVEVVVGARSAVFSPTPRLGLIILD
                     EEHESSFKQESAPRYHAREVAEQRARIEGIPLVLGSATPSLESWYRAQQGEAILVQLP
                     RRIANLPLPDVKTIDLREPKRARETRGVITRQLHAAIEEVLKDGGQVILLLNRRGFAT
                     HVQCPSCGVVLRCPHCDISLTHHKMANRVVCHYCDYEVETPRTCPQCDSPRIRFSGTG
                     TERLEAEIKARFPHATCLRMDADSMRSRLAYERAFAEFRRGRIQILVGTQIIAKGLDF
                     PNVRLVGVVNADTALHFPDFRASERTFQLVTQVAGRTGRGPHGGQVLVQTFTPEHPAI
                     QAAIRHDYETFAQQELKVREAFGYPPFWRLARIVVSSNSSETAEACSREFAASVHTQA
                     EVAGLTDKIRIVGPAPAPLAKLRGKYRYHLQVMARDYPQLLSVISTAQKTCRAPSGVT
                     WTIDVDPIDML"
     gene            1730..>2991
                     /locus_tag="PKI05_09200"
     CDS             1730..>2991
                     /locus_tag="PKI05_09200"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOA52423.1"
                     /translation="MSRVRTKMPQRRLNRALVETSLEVKCLFLFGVFLVLVIAVSVLL
                     YWRVTGEVVTRQNPDTARLLVDQVMLTKHYAAMEPNKDFVDYLNGVIKSRSQQGYRWR
                     FVRPPGRLMNQSEDTGPPLDAMESELIQRFMAMTEEERSHPDAQLYAERLVDGGNTYQ
                     YYQPIWAEQSCVPCHIALEGAGYSPTSGGIMTPIREGDLMAILQVTIPNGPTQAAVMK
                     YWSMLLAVAIVTAFLAMIAFYITIRYVIVRPLRHLRAVSEAITEGNTALRAHIHTRDE
                     FELLANAFNRMLSHLVAVQDELRQVNAELDAKLDEVAQLNMQLYEMNRVKSDFMATMS
                     HELRTPLNSILGFSEVLASIESLDEKQRRYVQNIQKSGRILLEMINNVLDLAKMEAGR
                     MEIRPTEFSIEQVVQAQCDMARPLTEKK"
BASE COUNT          684 a          805 c          853 g          649 t
ORIGIN      
        1 acaaaacgat tctaatccac ggggtaacag ggagcgggaa gactgaggta tatattcaag
       61 cgattgagga ggtcgttcgc cgcggttgtc aggcgattgt cctagtcccg gagatcagtt
      121 tgacacccca gacggtagaa cgctttcgaa gtcgcttcca caacgtggcg gtccttcaca
      181 gccacttaac agatgccgag cgggccgcac aatgggaatg gatcgcctca ggggcggtgg
      241 aggttgtggt cggggcacga agtgcggtat tttcgccgac cccgcggttg ggactgatta
      301 tcctcgatga agaacacgag tcgtcgttca agcaggagtc agctcctcgt tatcatgccc
      361 gggaagtggc agaacagcgt gccagaatcg agggaattcc cctcgtgcta ggatcggcga
      421 ccccctcttt ggaatcttgg tatcgggcac agcagggtga ggcgattctt gttcaactcc
      481 ctcgccggat cgccaacctc ccccttccgg acgtgaagac gattgatctg cgggagccca
      541 aacgggcaag ggagacccgc ggggtgatta cccgtcagtt gcatgcggcc atcgaagaag
      601 tactcaagga cggaggccag gtcatccttt tgctgaacag acggggcttt gccacgcatg
      661 tccaatgtcc ctcctgcggc gtggtgttgc gttgccccca ctgcgatatt agcctcaccc
      721 accataagat ggcaaatcgc gtggtttgtc attattgtga ctatgaggtg gaaacaccgc
      781 ggacatgccc tcaatgcgac tcgccgcgaa tccgttttag tggaacgggg acagaacggc
      841 ttgaggcgga gatcaaagcc cgctttcccc atgcgacctg tctgcggatg gacgccgatt
      901 ccatgagaag ccgcctggcg tatgaaaggg cgtttgccga gtttcgccga ggacgcatcc
      961 agattttggt gggcacacaa atcatcgcaa aagggctgga tttcccaaac gtccggcttg
     1021 tgggggtcgt caacgcggat acggccttac acttccctga ctttcgcgcg tctgaacgta
     1081 cctttcaact ggtcacccag gtggcgggcc gaaccggtcg gggcccccat ggtgggcagg
     1141 tcttggtaca gacattcacg cccgagcacc ccgccattca agcggccatc cgccatgatt
     1201 acgagacgtt tgctcagcag gagctgaagg tccgcgaagc ctttggctat cccccgtttt
     1261 ggcggttggc acgcattgtg gtcagttcga atagcagcga gacggcggag gcatgctcgc
     1321 gtgaattcgc ggcgtcggtc cacacccagg ccgaggtagc cggtctgacc gacaaaatac
     1381 gaatcgtcgg ccccgcgccg gcccctttgg caaagctccg cggcaagtat cggtaccacc
     1441 tccaagtcat ggcacgtgac tatccacaac tgctgagcgt cattagcacg gcccaaaaga
     1501 cgtgccgtgc gccatctggt gtaacctgga caatcgacgt tgacccgatc gacatgcttt
     1561 gagaggcgtt gaacgctcat gttcgtcggg aaatcgcgtc taatcgaggt ggtgcttggg
     1621 atttcgctcc tgtcccaacc cacgctttac tgtttcgcat tctcccataa ttgaataaga
     1681 tgaagaaaac cgatcaacaa cggcgattac cagccccata aggaatgtgt tgtctcgggt
     1741 cagaacgaag atgccccaac gacgattgaa tcgtgctctc gtcgaaacca gcctcgaggt
     1801 gaaatgcctc ttcctttttg gggtattttt ggttcttgtg atcgccgtca gtgttctcct
     1861 ctattggcgg gtcactgggg aagtggtcac gcggcaaaac ccggatacag cacggctgct
     1921 tgtggaccag gtcatgctca ccaagcacta tgcggcgatg gagccaaaca aagatttcgt
     1981 agactatttg aacggcgtca tcaagagccg ttcacaacaa gggtatcggt ggcgttttgt
     2041 gcgtccgccg ggcagactga tgaatcaatc ggaagataca ggaccgcccc tggacgccat
     2101 ggaatccgag ctcattcaac ggttcatggc catgactgag gaggaacggt cgcaccctga
     2161 tgcccaactc tatgctgagc gattagtcga tggcggaaac acctatcaat actatcagcc
     2221 gatttgggca gaacagtcgt gtgttccctg ccacatcgct ttagagggtg cgggctacag
     2281 cccaacgtcc ggaggcatta tgacgcccat tcgtgaagga gacctgatgg ccatcttgca
     2341 ggtcacgatc cccaatgggc cgacacaggc tgccgtcatg aagtattgga gcatgctcct
     2401 ggctgtcgcg attgtcacgg cttttttagc aatgatcgcg ttttatatca caatccgcta
     2461 tgtcattgta cgaccgttgc ggcacctgcg ggctgtaagc gaggcgatca ctgagggcaa
     2521 tacggcatta cgggcgcata ttcacacgcg agatgaattc gagcttttgg caaatgcttt
     2581 caaccgcatg ttaagtcact tggtcgcggt tcaggacgag ctccgtcagg tgaacgccga
     2641 actggatgcg aagctcgacg aggtagctca attaaacatg caattgtacg aaatgaatcg
     2701 cgtgaaaagc gatttcatgg ccaccatgag ccatgaactg aggacacctc ttaatagcat
     2761 tttgggattc agcgaagtcc tggcctcgat tgagtctctc gatgagaaac aacggcggta
     2821 tgtgcagaac attcaaaagt cgggtcgtat ccttctggaa atgatcaaca atgtcctgga
     2881 ccttgcgaag atggaagcag ggcgcatgga gattcgccca accgagtttt ccatcgagca
     2941 agtcgtccag gcacaatgcg acatggcacg gccattaaca gaaaagaaga a
//
LOCUS       DAOPTR010000452         2968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_26640_length_2968_cov_3.760041, whole genome shotgun sequence.
ACCESSION   DAOPTR010000452 DAOPTR010000000
VERSION     DAOPTR010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26640_length_2968_cov_3.760041"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PKI05_09205"
     CDS             complement(<1..356)
                     /locus_tag="PKI05_09205"
                     /inference="COORDINATES: protein motif:HMM:NF021236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated Cmr3 family
                     protein"
                     /protein_id="HOA52424.1"
                     /translation="MNQSILSAKTRLVALRLDPLDVLFFRDGRPFHEASRGESGLPRP
                     QTLAGALRTHLLRRAGCDGDGFRKLGEYLRAGQSLEQAITSVCGAGWIARVEVRGPWL
                     CEEVNGTLEILVSIPA"
     gene            complement(356..>2968)
                     /gene="cas10"
                     /locus_tag="PKI05_09210"
     CDS             complement(356..>2968)
                     /gene="cas10"
                     /locus_tag="PKI05_09210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR-associated protein Cas10/Cmr2"
                     /protein_id="HOA52425.1"
                     /translation="LVVAYSHLPPDRTLAIDGSESRACRYASIAAGREVGAEEVRANA
                     NQLGRNLERFPMPSEQKVGRLRIGPKDKRLTIIHPLSGQSSELEVGTLEEEKVAAAIK
                     ELLDGIDSPKRRFLALWRLLPERLARIGAWYRLLPADLRNPDHTIWHYLDAAAGLKMA
                     ISEGDEGALLSFALGPVQSFIEAARSVRDLWAGSMLLSWLTFQGMLPILEAYGPTAIL
                     FPSLREIPLVDWWLIEQGLNKVDVPRSEWIRLPCLPNRFLALVPCESGGEQAEDLAER
                     CQLAVRDAWRGLAKSVREEIERSLAKLGEDFSGWVARWDTQIEGFFSVTTAVLPLRQC
                     NEAMIARLFGASNFDEAFPKLAAVRRLAEAIPPGDRPGYDQTSAGRWQAQVEMSARLM
                     EALRAVRHIPPSTRDMPVPGKCSLMGTFEQMGPAKLEESRRFWETMAQCVHIGLDRIR
                     KRERLCGVAIVKRFAPHFLAQKLKIDERLLRTPDTATVAATRWLKEACDRGYELDPEA
                     IRERYDVWSGQWLFWTRKDQGQDEDEDAVPDDVWEEIQKARKDKSLGPPPTYYAILMM
                     DGDWMGRWLSGEMAPPIENVLHPETLSYFRQLNDPGAEAALRAPRPLGLVDHAVMSQA
                     LTNFALFVVPKIVKEHHGTLIYAGGDDVLAMLPASTAVACARTLRLAFSGDTRVNGGA
                     RPGYYRLGNRELLMMGPRATSSCGIAVIHYKEDLRFALQQARRAEKAAKRAGRNLLQI
                     AICRRSGEHTSVLCPWEFVPTVERWVQAFLRGASDGWTYRLRAELKTLAAVSREAVRA
                     ELSRLVERSEQSTKDLLSESENISAGQMLVRQFDDYLSQLLAPPRSLDEEQAMEGFVH
                     LCQAASFLARGRTE"
BASE COUNT          642 a          955 c          770 g          601 t
ORIGIN      
        1 tttgcaggga tcgagaccag aatttcaagt gtgccgttga cctcttcgca aagccaggga
       61 ccacggactt cgaccctcgc aatccagccg gcgccacaga cggatgtgat tgcctgctcc
      121 agagattggc cagcccgcag gtactccccc aatttacgga aaccgtcccc atcacaccct
      181 gcgcgacgta agagatgagt tcgtagggcc cccgccagcg tctgaggtcg gggcaaccct
      241 gactccccgc gtgaggcctc gtgaaacggc cggccatcgc ggaaaaaaag cacgtccagc
      301 ggatcgagtc gcaaggccac cagccgagtt ttggcagata aaatcgattg gttcatcact
      361 cggttctccc ccgggccaaa aatgacgccg cttggcaaag gtggacgaag ccctccatag
      421 cctgctcctc atcgaggcta cgcggcggcg ccaaaagctg tgacaaataa tcgtcgaact
      481 gcctgacaag catttggccg gccgagatat tttcgctttc gctcagcagg tccttcgtgc
      541 tttgttcaga acgttcaact aggcgtgaca actcggcccg cacagcttcc cgagataccg
      601 cagcaagggt cttcagctcc gcacgcaacc ggtaagtcca gccatccgag gcgcccctca
      661 aaaaggcttg aacccagcgt tccacagtgg gtacgaactc ccaggggcaa aggaccgagg
      721 tgtgttcgcc cgaccggcga cagatggcaa tctgaagcag gttgcggccg gcacgtttcg
      781 cggccttctc ggcccgtcgg gcctgttgaa gggcaaatcg caggtcttct ttgtaatgga
      841 ttacggctat tccgcacgag ctagttgccc ttgggcccat catcaacaat tcgcggttcc
      901 ccagcctgta gtaacctggc cgggcaccac cgttgacccg tgtgtcaccg ctaaaggcaa
      961 gccgcaaggt gcgggcgcag gccaccgctg tcgaggccgg tagcatggcc aggacgtcat
     1021 cgccacctgc gtagatcagc gtcccgtgat gctctttaac aatcttcggc accacgaaaa
     1081 gcgcaaaatt ggtcagggct tgactcatca ctgcgtggtc caccaagccc aagggacgcg
     1141 gtgccctcaa agccgcttcc gcccctgggt cgttcaactg ccggaaatag gatagcgtct
     1201 cggggtgtaa cacgttttcg atcggtggtg ccatctcccc agacaaccaa cgtcccatcc
     1261 agtcgccgtc catcatcaaa atcgcgtaat aggtcggcgg tggaccgagc gacttatctt
     1321 ttcttgcttt ctggatttcc tcccacacat cgtccgggac cgcgtcctcg tcttcgtcct
     1381 ggccttggtc ttttcttgtc cagaacagcc attggccact ccacacgtca taacgttcac
     1441 ggattgcctc gggatcaagt tcatagccgc ggtcgcatgc ttccttcagc caccgggtcg
     1501 ccgccaccgt agctgtgtcg ggcgtacgca acaaacgttc gtcgattttc agcttttggg
     1561 ctagaaaatg cggtgcaaat cgcttaacga tggctacccc acaaaggcgt tctcgcttgc
     1621 gaattcgatc caacccgatg tggacgcact gggccatggt ttcccaaaat cggcgcgatt
     1681 cttccaactt cgctggtccc atctgctcga aggtgcccat caaactacat ttgcctggca
     1741 caggcatgtc ccgtgtcgaa ggcggaatat ggcgaacggc gcgcagagcc tccatgagtc
     1801 gcgccgacat ttcaacctgg gcctgccatc gaccggccga agtttggtca taaccggggc
     1861 gatctcccgg aggaatagct tcggccaacc gacgtacggc ggccaacttc ggaaatgcct
     1921 catcgaaatt ggacgcaccg aataagcggg cgatcattgc ctcattgcat tggcgaagcg
     1981 ggagcaccgc cgtcgttacg gaaaagaaac cttcgatctg tgtgtcccat cgtgccaccc
     2041 agcccgaaaa gtcttcccct aacttcgcca gcgatctttc gatttcctcg cgaacagatt
     2101 tggccaaacc tcgccatgcg tcccgcaccg ccaattggca ccgttcggcc aagtcttcag
     2161 cttgttcccc tcccgattcg cagggaacca aggccaagaa tcgattcggc aagcaaggca
     2221 agcgaatcca ttccgaacgt ggcacatcca ccttattcag gccctgctcg atgagccacc
     2281 agtccacgag aggaatttcg cgtagagaag gaaaaaggat tgccgtcggt ccatacgcct
     2341 ccaggatcgg caacatccct tgaaaggtga gccatgatag cagcatgctt cctgcccaaa
     2401 ggtcccgcac ggaccgagcg gcctcaataa aggattgaac cggccctaag gcgaacgaaa
     2461 gcaaagcccc ctcatccccc tcagatatcg ccatctttag ccctgccgct gcatctaggt
     2521 aatgccaaat cgtgtgatcg gggtttctta ggtctgcagg cagcaagcgg taccatgccc
     2581 caattcgtgc cagacgctcg ggcaggagcc gccacaacgc cagaaaccgt cgcttcggcg
     2641 aatcaatccc gtcgaggagc tccttgatcg ccgccgcaac tttctcctcc tcaagtgtcc
     2701 cgacttctaa ctcggaactc tgaccactca gagggtgaat gatcgtcaac cgcttatctt
     2761 tcgggccaat cctcagcctc cccacctttt gctcggaagg catcggaaac cgctccaggt
     2821 tccggccgag ctgattagcg ttggcacgaa cctcctccgc tcctacctcc cgccctgccg
     2881 caatgctggc atagcgacat gcccgcgatt ctgatccatc aattgccaac gttctgtccg
     2941 gtggaagatg cgaataggcc acgaccaa
//
LOCUS       DAOPTR010000453         2967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_26647_length_2967_cov_4.021978, whole genome shotgun sequence.
ACCESSION   DAOPTR010000453 DAOPTR010000000
VERSION     DAOPTR010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26647_length_2967_cov_4.021978"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /gene="truB"
                     /locus_tag="PKI05_09215"
     CDS             complement(<1..753)
                     /gene="truB"
                     /locus_tag="PKI05_09215"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOA52426.1"
                     /translation="MEGHTRTICGVFCLNKPAGITSRDAVNLVQRSLRRVRLGHAGTL
                     DPLATGVLVVCAGLATRLIPFIQELPKLYRATFLLGRSSPTDDVDGEVTVFEDAPVVT
                     REQVESTLPEFVGTVLQSPPIYSAVKIEGRRAYEWARKGAPREPKPRPVEIHSLKLLR
                     YEYPELELEIHCGSGVYVRSIGRDLAQRLGTAAVMSRLTRLAIGHFVIDQAVDPHEIG
                     PHNWSEYLMPPAVAVKHLRQIQLSEDHIAQLVH"
     gene            1009..1944
                     /locus_tag="PKI05_09220"
     CDS             1009..1944
                     /locus_tag="PKI05_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HOA52427.1"
                     /translation="MARPLSKLAKDWWDYTTLDPELLQDAAKLTVRQVERLSRPGFKV
                     VMYDTLEEFYLAEALEYIHAWKQSTADNPCGICGPIGPTEQLPLVARLVNELELDVRD
                     GHFWGMDEWVDELTGEPVPMDHPLSFARADMELCFKRINPRLRMPKEHIRFPIGDLEA
                     YSRSFDEIRCVIMQGGQGDVKHWAFNDPPKRQGRWKDAPPPPSEYRKLKTRITDLHPA
                     TIMQNARTSGGGQVHLVPTKAATVGPVETWKAEKVSIWHAGMHDNPFGMRLTALMISK
                     GIADSAVPMSLLADHPNVQFNFYRPALAEVKTEMH"
     gene            2041..>2967
                     /locus_tag="PKI05_09225"
     CDS             2041..>2967
                     /locus_tag="PKI05_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52428.1"
                     /translation="MSTIFSATILIAFGLWAANSEQQTVIRNGDWVVTDHETVADQHI
                     VLNGSLILEKGGQLTLRNCHLEIIGEKSREHIVDWRGGKLVTLKTVLGGTDRTGTAVH
                     TVFHLYDGIWEAEDTTVEFSYGISFSDTARGILRAKRLRAGRRPDAVIASGLADITLE
                     DSVFPVALGIYTHQGGDVTLALPSKRPLDASFDSTNLTPGVKWKLTLVRTEVPQWFVF
                     LRNIGPSNPPCVVTLQEADDTIISLLGHNLAGDIFLTPNLSEPLRLGSLTLKRGSAEI
                     RVPMWALYFSGDKTDVNIRGKTHICELMVRAGK"
BASE COUNT          690 a          775 c          858 g          644 t
ORIGIN      
        1 gtgaacgagt tgtgcgatat ggtcctcgga aagttgaatc tggcgaagat gctttacggc
       61 cacggcaggc ggcataagat attccgacca attgtgggga ccaatctcat gcggatcaac
      121 cgcctggtcg atcacaaaat gcccgatggc caaccgcgtt agccgcgaca tgaccgccgc
      181 cgtccccaac cgctgagcca aatcccgccc aatcgaccgg acatacacgc ccgagccgca
      241 gtggatctcc aattccaact ccggatactc gtaccggagg agcttcaagc tatgaatctc
      301 caccggtcgt ggttttggtt cgcggggggc accttttcgg gcccattcgt aggctcgtcg
      361 gccttcgatc ttgacggcgg aatagatggg tgggctttga agcaccgtcc cgacgaattc
      421 aggcagcgtg gattccactt gttcacgcgt aacaacgggg gcatcttcga aaacggtgac
      481 ctccccgtcg acgtcgtcag tgggactcga tcgccccagc aagaatgtag cgcgatacag
      541 cttgggcaat tcctgaatga aggggatcaa acgtgttgcc aagcccgcac agacgaccaa
      601 cacgcctgta gcaagaggat caagtgtgcc ggcatgaccc aatcgcaccc ggcgtaaaga
      661 tcgctgcacg aggttcactg cgtcgcgaga tgtgatgccc gccggcttgt tgaggcaaaa
      721 aactccacaa atcgtgcgag tgtgtccttc catcgtcaaa cggcgttaac catacgagag
      781 tggaaacaac cgaggaaagt tcggaaaaac aagattctcc cgaggggaat atgctcgttc
      841 caaagtttca tgataggaga tttttccgga gcgataatgc ccccggcttg gcggttgtgc
      901 cctggcaaac gacggcacgt cattgacggg aagagaattt tcctgataat ggggctaaaa
      961 atcgtccggc acgaagatgg tcgaaactct tctcgaaagg agtgcatcat ggcaaggcct
     1021 cttagcaaat tggcgaaaga ctggtgggat tacacgacgc tcgatccgga gctgctccaa
     1081 gatgcggcaa agttgacggt gcggcaggtc gaacgccttt cgcgacctgg cttcaaggtt
     1141 gttatgtatg acaccttgga agaattctat ttggcagaag cgcttgagta tattcacgcc
     1201 tggaagcagt ccaccgctga taacccctgc ggcatctgtg gcccgattgg accgacagaa
     1261 caactgccac tggtcgcccg cttggtcaat gaacttgagc ttgacgtgcg cgatggccac
     1321 ttctggggta tggacgagtg ggttgatgaa ctcacagggg agccggtccc gatggatcat
     1381 cctttgagct ttgcccgggc cgatatggag ctgtgtttca agcgaatcaa tccccggttg
     1441 cggatgccca aagagcatat ccgctttccg atcggggacc tggaggcgta ttcgcgaagt
     1501 ttcgacgaga ttcgctgcgt gatcatgcag gggggacagg gggatgtcaa acactgggcg
     1561 ttcaacgatc cgcccaagcg tcaaggccgt tggaaggacg ccccgccacc gccttcagaa
     1621 taccggaaat tgaagacacg aatcactgat ttgcatcctg ccacgattat gcaaaatgcg
     1681 cggacgtcgg gaggtggtca agtccatctc gtgcccacca aggcggccac cgtgggaccg
     1741 gtggaaacgt ggaaggccga aaaggtctcc atctggcacg cggggatgca tgacaacccc
     1801 ttcgggatgc ggctcacggc cctgatgatc tccaagggga ttgccgactc tgccgtgcct
     1861 atgtcgttat tggctgacca tcccaatgtc cagttcaact tttaccgtcc ggccttggcg
     1921 gaagtaaaaa ccgagatgca ctgaggtgct cggctcgcgg atctatcacg caaaacatcg
     1981 ggctacccga ggcgggaggt attttgcaca aaatgttgag ggtcaaggtg tcgaacgacg
     2041 atgagcacaa ttttttctgc cacaattcta atagcattcg gtctttgggc tgcgaattct
     2101 gagcagcaaa ccgttattcg caacggtgat tgggttgtca cagaccatga gactgtggcc
     2161 gatcagcaca ttgtgttgaa cggcagtttg attctcgaaa agggtggaca gttaacgttg
     2221 aggaactgcc acctcgagat cattggcgaa aagtctcggg agcatatcgt tgattggcga
     2281 ggtggaaaac tcgtaacctt gaagaccgtc ctcggtggca cggatcgtac gggaacagcc
     2341 gtgcacaccg tgttccattt gtacgacggc atttgggaag ccgaggatac gaccgtggag
     2401 ttttcctacg gcatcagctt ctcagatact gcccggggaa ttcttcgcgc gaaacgattg
     2461 cgggcagggc ggcgaccaga tgccgtgatt gcctcaggct tggccgacat cacgctggag
     2521 gacagcgtct ttccggtggc gctgggtatt tatacgcatc aaggagggga tgttaccctc
     2581 gcattaccgt cgaaacggcc ccttgacgct tctttcgaca gcacaaacct gactccgggc
     2641 gtgaaatgga aattgacttt ggtgcgaacg gaagtgcccc aatggtttgt gttcctgcga
     2701 aatattggcc ctagtaaccc gccttgtgtc gtcactttgc aagaggccga tgatacgatc
     2761 atctctttgt tggggcacaa tcttgcggga gatatttttc ttacaccgaa cttgtcggaa
     2821 ccactgcgcc tcggttcgct tacattaaaa cggggctcgg cagaaatcag agtgcccatg
     2881 tgggcgcttt atttttctgg ggacaaaacc gacgtgaaca tccgcggaaa aacgcatatc
     2941 tgcgaactga tggtgcgcgc gggaaag
//
LOCUS       DAOPTR010000454         2966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_26660_length_2966_cov_3.751975, whole genome shotgun sequence.
ACCESSION   DAOPTR010000454 DAOPTR010000000
VERSION     DAOPTR010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26660_length_2966_cov_3.751975"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PKI05_09230"
     CDS             complement(<1..412)
                     /locus_tag="PKI05_09230"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOA52429.1"
                     /translation="MSKTTARLCVLAAVILWSSSGFFTKSHFFDDWPVEWRGAMFGFW
                     RAVAATCVLLPAVRGVRFRPMLIPMVISFAGMNITFLSAMVQTTAANAIWLESTAPFW
                     VLLASVLIFRERLRKREFVPFIAAFLGVGLILGFE"
     gene            complement(541..>2966)
                     /locus_tag="PKI05_09235"
     CDS             complement(541..>2966)
                     /locus_tag="PKI05_09235"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOA52430.1"
                     /translation="SSSSSVFKIVCFGFVHVIPIYGARGAESQYIPYLSPQWMEMLDY
                     LVRRAESLGMFVDMTTGTGWCFGGPNLPDWANDARAQYDAASGAVTLVPAMQVKRAAP
                     GGEGPMLNPFSPRAMDLYLERFSAAFDSSRVARPRAQYHDSFEYQSNWSADLFEEFRS
                     CRGYDLKEHLSLFFDESRSADEERARLKCDYRWTLAELHLELIRRWVAWAHHHGMLTR
                     NQAHGAPGNLLDLYAASDIPETEMFGAPELPIPGFRRDPAMVRAADDDPRICLLASSA
                     AHVAHPRGQQLVSAETCTWLREHWHTSLAQIKLQLDLFFLTGVNQLLFHGTCYSPKDA
                     PWPGWFFYASIKMDWRNAFWRDVPVLTQYVARCQSVLQAGQHDNDVLLYWPIFDLWMD
                     PKGMTIPLTVHRRAWMAEQHVGEVANRLLQRGFAFDFISDRMIAELKASSGMIEAPGG
                     VYRALLIPACQYMPEETLGHLADLAEAGVAVIFERQLPKDVPGLSDLPARRARFAEHR
                     GRLEQARTIAEDIEKALIAAGAIREPMADSGLRFVRRKIGQDTWYFVANHTPQPVDGW
                     MPLGISGQGVTQFDPMTGHSGCLACRQRDGMMEVYLQLEPGCSRFVRVTREASSGPTW
                     RYLRPKGEPTVLTGTWSVEFIEGGPTLPKSYQTDKLASWTEAPDTAAQAFAGTARYTL
                     EFTAPTETGTEWLLDLGDVRESARVRLNGRDMGTLIALPFRVCLGDALKPGHNRLEVE
                     VTNLSANRIRDMDLRKVDWKIMKDINIVNVNYQKFDASQWPIEPSGLLGPVRLIPLEH
                     FDPTGSGNP"
BASE COUNT          686 a          907 c          823 g          550 t
ORIGIN      
        1 gctcaaaacc gagaatcagc cccacaccca aaaaagccgc aatgaaaggc acgaattcgc
       61 gcttccgcag cctttctcga aagatcagaa ccgatgccag caagacccaa aaaggggccg
      121 tgctctctaa ccaaatcgcg ttggctgccg tcgtttgcac catcgcacta agaaaggtga
      181 tgttcattcc ggcgaaactg atgaccatcg ggatgagcat gggacgaaag cggacaccac
      241 ggacggcggg cagcaaaaca cacgtggcag ctaccgcccg ccaaaaccca aacatggctc
      301 cgcgccattc gacaggccaa tcatcaaaga agtgcgattt cgtgaaaaag ccactgctac
      361 tccagaggat cactgcggcc aaaacacaga gtcgcgccgt tgtcttgctc atcgtcgtgt
      421 gcacgatttc cccaaccagc gaaaggcaaa ccctcgcaac ctatgaggcc cttaaatgcc
      481 gttcccataa ggcaaatgcc ccttgtcatg cgaaccgaaa cagccagagg ctttgcctcg
      541 tcacgggttc ccggaccccg tgggatcgaa gtgttccaag gggatcagcc gcaccggacc
      601 cagcaggccc gatggctcga tgggccattg cgaggcatcg aatttctgat agttcacgtt
      661 cacaatgttg atatccttca tgatcttcca atcgactttc cgcaggtcca tatctcggat
      721 ccggttcgcc gacaggttag taacctccac ctccaggcgg ttgtgtcctg gttttagggc
      781 atctcctaag cacacgcgaa atggcagggc aatgagcgtc cccatatccc tgccgttaag
      841 gcgaactcga gcactctcgc ggacatcccc caaatccagg agccactccg tgccggtctc
      901 cgtcggggca gtgaactcca gcgtataacg cgccgtcccc gcaaaggcct gggcggccgt
      961 gtccggagcc tccgtccacg aggcgagctt atccgtctga tacgactttg gcagcgttgg
     1021 cccaccttca ataaactcca ctgaccacgt cccagtgaga acggtcggtt cgcctttggg
     1081 gcggaggtaa cgccacgttg ggccggaact ggcttcacgt gtcactcgga cgaacctcga
     1141 gcatcccggc tccaactgaa gatagacctc catcatcccg tcgcgctggc ggcatgccaa
     1201 gcatcccgag tggccggtca tgggatcgaa ttgcgtaacc ccctggccgg aaataccgag
     1261 cggcatccaa ccatcgacgg gctggggcgt gtgattggcc acaaagtacc acgtatcctg
     1321 cccgatcttg cgccgcacaa atcggaggcc ggaatcggcc attggttccc gaatagcccc
     1381 cgccgcgatc agggcctttt cgatgtcctc cgcaatggtc cgcgcttgct caagccgtcc
     1441 gcggtgctct gcaaatcggg cccggcgtgc gggaaggtcg cttagtcccg gcacatcctt
     1501 gggcaattgt cgctcgaaga tgacggccac ccccgcctcg gccaaatccg ccagatgccc
     1561 caaagtttct tcgggcatat actgacaggc ggggatgagg agcgctcgat aaaccccgcc
     1621 cggggcctcg atcatcccgc tgctcgcttt cagctctgcg atcatgcggt cagaaataaa
     1681 atcgaaggca aatccccgtt ggaggagccg gttggcaacc tcgcccacgt gttgttctgc
     1741 catccaggca cggcggtgaa ccgtcagcgg aatcgtcatc cccttcgggt ccatccacaa
     1801 gtcgaaaata ggccagtaga ggagcacgtc attgtcatgt tgcccggctt gcaggaccga
     1861 ctggcatcgt gctacatatt gcgttagaac cggaacgtca cgccaaaagg cgtttcgcca
     1921 gtccattttg atcgaggcgt agaagaacca gccgggccat ggggcatcct tgggggaata
     1981 acatgtgcca tgaaacaata gttgattaac gccagtcaaa aagaacaaat ccaactgcaa
     2041 tttgatctgg gccaggctgg tgtgccaatg ctcgcggagc cacgtgcagg tttcggcgct
     2101 gaccaactgc tggcctcggg gatgcgccac gtgggccgcc gatgacgcca aaaggcaaat
     2161 gcgtggatcg tcatcggcag cgcggaccat ggcgggatca cgacggaagc cggggatcgg
     2221 aagctccggt gcgccgaaca tttccgtctc gggaatgtcg gaggcggcat acaagtccag
     2281 cagatttccc ggcgcgccgt gggcttgatt ccgcgttaac atgccgtggt ggtgtgccca
     2341 agccacccac cggcgaatta actcgaggtg caactcggcc aatgtccagc ggtaatcgca
     2401 ctttagccgt gcccgctcct catcagcgga acgagactca tcgaagaaga gcgataggtg
     2461 ctctttcaga tcataaccgc ggcaagagcg aaactcctcg aagagatccg cggaccaatt
     2521 agactgatac tcgaatgagt catgatactg cgcacgaggt cgagccacac gtgagctgtc
     2581 gaacgcagcc gaaaagcgtt ctagatacaa gtccatggcg cggggtgaga agggattgag
     2641 catgggacct tcgccgccgg gcgccgcccg cttgacctgc attgccggta ccagcgtaac
     2701 tgcccctgat gcggcgtcgt attgggccct ggcatcgttg gcccaatcgg gaaggtttgg
     2761 cccgccaaag caccaacccg tccctgtggt catatccaca aacattccca acgactccgc
     2821 tcgtcgaaca agataatcga gcatctccat ccactgtggc gagagatagg ggatatattg
     2881 ggactccgcc ccgcgggcac cgtaaatggg tatgacatgg acgaagccaa aacaaacgat
     2941 cttaaagacc gatgaggaag aactga
//
LOCUS       DAOPTR010000455         2962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_26702_length_2962_cov_4.405229, whole genome shotgun sequence.
ACCESSION   DAOPTR010000455 DAOPTR010000000
VERSION     DAOPTR010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26702_length_2962_cov_4.405229"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PKI05_09240"
     CDS             <1..324
                     /locus_tag="PKI05_09240"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOA52431.1"
                     /translation="AVRQRIMDTFTPGEESEQALVALAEAYQAVRRYASAAEIYLQAA
                     QKSDHPEPLLLAAAENYYRAGQPGQALGITEQILASNPQNHRARDLQGRLQGVTVASQ
                     SPIFR"
     gene            498..2231
                     /locus_tag="PKI05_09245"
     CDS             498..2231
                     /locus_tag="PKI05_09245"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HOA52432.1"
                     /translation="MSHPSDSRDDHAPGEGVPPPGLDERAADGAGAIQSQQPALSKPA
                     EQPSQPAAKDVPPKRKRPVVLRAPLAKSARVVEEAQIAAMLPTEVATPITPHTPRSRD
                     RYRFFASCLISAVAHAAVLIALSLWMLNLPQGLGPTSLFATWAGQESLEGPEAELVLQ
                     SESQELTPKETELALQQSRINVLESSPLEENNSDEPTAQVADVGDPARPWGVQGATLM
                     QSVERPTGGGLEGRSPEMRQKLAQSGGGTAQSEEAVERGLRWIAAHQDQDGGWSFDLK
                     KGSCRGQCRNPGTEPNTAAATAMALLPFLGAGYTHREGIYEDTLHRGLYYLLRRGRVT
                     ENGLDFQEGMKNGMYAQGLAALVLCEAYAMTKDPALKDPAQQAIRFIESAQDKKGGGW
                     RYRPGEPGDTTVTGWQLMALKSAEMAGLKVDRSVHYAAQRFLDSVASDDGSQYGYLDK
                     TPRRSTTAIGLLCRMYGGWVRGNPPLVRGIGHLASWGPSATDIYYNYYATQVLRHWGG
                     SDWKRWNDAMREYLINMQDTKGHESGSWYFDDEHSRVGGRLYTTAMAVMILEVYYRYM
                     PLYGKQVFPEL"
     gene            complement(2226..>2962)
                     /locus_tag="PKI05_09250"
     CDS             complement(2226..>2962)
                     /locus_tag="PKI05_09250"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52433.1"
                     /translation="LTEDDAHREDSPIARVFYNLQKRHAGHWHHVTSQDELIAQGDSR
                     PAFLQKGAWSHASAGLIEQLWVIVTRDDLWRLREQLSRRSQQSNEPWFAERYIEGREF
                     NLSLIAGERGPIVFPPAEIDFSAFPPEKLKIVGYSAKWDADSFEYVATPRRFVFPAED
                     QPLLLQLEALARQCWQVFGLRGYARIDFRVDAAGQPWILEINTNPCLSPDAGFAAAAA
                     EAGWSFDRVTEAILADALRAAGQTAS"
BASE COUNT          639 a          915 c          870 g          538 t
ORIGIN      
        1 gccgtccgcc agcggatcat ggataccttc acaccggggg aggagtcaga acaggcccta
       61 gtagcgttgg ccgaggccta ccaggccgtc cggcgctacg cttcggcggc tgaaatctac
      121 ctccaggccg cccaaaagag tgatcacccg gaaccgctcc ttctcgccgc agccgagaat
      181 tactaccggg ccggccagcc tgggcaggcc ctgggcatca cagaacaaat cttggcctcc
      241 aatccccaaa accatcgtgc ccgcgattta caaggtcgcc tccagggtgt caccgtagcc
      301 tcccaatcgc ccatctttcg gtgaacgcac ctggccgaaa atagccgacc atatcgttcc
      361 ggttgcacag agcaccacct tgggccgaga taccccttac cagcccgcga tgcccgggat
      421 aatcacgggg tataattcca aaggttacct gctttgccta ttctgccccg tccctgaagg
      481 aatagtcgaa ctcgctcatg tctcacccgt cggatagccg cgacgatcat gcaccagggg
      541 aaggcgttcc gccccctggc ctggatgaac gtgcggcaga tggggcggga gcgatacaat
      601 cccaacaacc ggctttatcc aaacctgcgg aacaaccctc ccaacccgcc gcgaaggacg
      661 tgccgccaaa gcggaagcga ccggtagtgc ttcgggcacc gctggctaag tctgcccgcg
      721 tggtggaaga ggcccaaatc gcggccatgc tcccgactga ggtggcgacg ccaatcacgc
      781 cgcacacacc acgtagtcgg gatcgctacc gctttttcgc cagttgcttg atcagtgccg
      841 tggcccacgc tgccgtgctc atcgcgcttt cgttatggat gttgaacctg ccgcagggac
      901 tggggcccac cagtcttttt gccacctggg caggacaaga atccctggaa ggccccgagg
      961 cagagcttgt cctccagtcg gaatcgcaag agttgacccc aaaggagacc gagctggccc
     1021 ttcaacagtc gcggatcaac gtcttggaga gctcccctct cgaagagaac aattccgatg
     1081 aacccactgc gcaggtggcg gacgtcggtg atcctgcgcg gccatgggga gttcaagggg
     1141 ccaccttgat gcagtctgtc gagcgcccaa caggtggcgg cttggaaggc cgatctccag
     1201 agatgcgaca aaaactggcc caaagtggcg gaggaacagc ccagagtgag gaggccgtgg
     1261 aacgcggtct tcgctggatt gccgcccatc aggaccaaga tggcggctgg agcttcgacc
     1321 tgaaaaaagg aagttgccgg ggccagtgtc gaaatccagg gacagaacct aatacggcgg
     1381 cggccacggc catggccttg ttaccctttc tcggagcggg ctatacccat cgggaaggaa
     1441 tctatgagga cacccttcac cgcggactgt actaccttct gcgtcgtgga cgtgtcacag
     1501 agaatggcct ggactttcaa gagggcatga aaaatgggat gtatgcccag ggtttggcgg
     1561 ctttggtcct ttgcgaggcg tatgccatga ccaaagatcc ggcccttaaa gacccagcgc
     1621 agcaggcaat ccggttcatt gaatccgctc aagacaaaaa aggcggcggc tggcgctatc
     1681 ggccaggcga acccggtgat acgacggtca ccggctggca attgatggcc ctgaaaagcg
     1741 ccgagatggc cggcttgaag gtcgatcgct ccgtgcatta cgcggcgcag cgatttctcg
     1801 attccgtcgc cagtgacgac ggttcccagt acggttatct cgacaaaacg ccgcggagga
     1861 gcaccacggc gattggtctg ctctgtcgta tgtacggtgg ctgggtccgg gggaatccgc
     1921 ccctggtccg cggcatcggc catctcgcct catggggacc atcggccacc gacatctatt
     1981 acaactacta cgcgacgcag gtacttcgtc attggggtgg ctcagactgg aaacgctgga
     2041 acgacgccat gcgcgagtat ctcatcaaca tgcaggacac caagggacat gaatcaggaa
     2101 gctggtactt cgacgatgaa cactcgcggg tcggcggccg actttataca accgccatgg
     2161 ccgtcatgat cctcgaagtg tactaccggt acatgcccct ctacggcaag caggtcttcc
     2221 cggagttatg aggccgtttg ccccgcggcc cgcaaggcgt cggcgaggat ggcctccgtg
     2281 acccgatcga atgaccagcc ggcctcggcg gccgccgcag caaacccggc atcaggactc
     2341 aggcaagggt tggtgttgat ctccaaaatc caaggctggc ctgccgcatc cacccgaaaa
     2401 tcgattcgcg cataaccccg aagaccaaag acttgccagc actgccgagc tagggcctcg
     2461 agctgcaata gcagcggttg gtcctccgcg gggaagacga aacgccgggg cgtcgccaca
     2521 tactcaaatg aatcggcatc ccacttcgcg ctgtagccga cgatcttcaa tttctcgggt
     2581 ggaaaggcag agaagtcgat ctcggcggga ggaaacacga taggcccccg ttcacccgca
     2641 atgagcgaga gattaaactc tcgtccctcg atgtagcgct ccgcaaacca cggttcgttc
     2701 gactgttgag agcgtcggga aagctgctct cgtagccgcc acaaatcgtc cctcgtgaca
     2761 atcacccaaa gttgctcaat cagccccgcc gaggcgtgcg accaggcgcc cttctgcaga
     2821 aacgccgggc gtgaatctcc ttgggcgata agctcgtcct ggcttgttac gtgatgccag
     2881 tgaccggcgt ggcgcttttg caggttatag aaaacacggg cgatgggact gtcctcacga
     2941 tgggcgtcgt cttccgtgag tt
//
LOCUS       DAOPTR010000456         2960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_26743_length_2960_cov_4.006196, whole genome shotgun sequence.
ACCESSION   DAOPTR010000456 DAOPTR010000000
VERSION     DAOPTR010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26743_length_2960_cov_4.006196"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..1489
                     /locus_tag="PKI05_09255"
     CDS             224..1489
                     /locus_tag="PKI05_09255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOA52434.1"
                     /translation="MDGWPEVVITGVGIVSPIGIGCEAFWESLCAGRSGITKIEALRE
                     QAWASSIGGVVTNFDAKDRVRPRKALKVMSRDIQMGFVAADLAVEMSGIKSSGVEPER
                     LGIVFGADLIPCELDEIQNAIRRCIVDGHFDFSLWGQYAMSDLYPLWLLKYLPNMPAC
                     HIGIAHDARGPNNTLTLAEVSGLASLIEAVRVIQRGEADVMIAGGTGSRINPSIWVRE
                     GAYELSKRVDDPQHACRPFDAARDGMIHGEGAGAVVLERRDHAVARGARILAQVLGYA
                     CTFEPRRLHHPVQGTAIRQAIRLSLRMADLAPRDVGHVNAHGVSTVDDDRAEAKAIHS
                     ELGDVPVTALKSFFGNLAAGTGIVEMAGSLLAFAGKKIPFTLNYETPDPECPIDVVAK
                     APRPLKIPTAVILNHSRIGQAVSLVLAAG"
     gene            1571..2107
                     /gene="purE"
                     /locus_tag="PKI05_09260"
     CDS             1571..2107
                     /gene="purE"
                     /locus_tag="PKI05_09260"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOA52435.1"
                     /translation="MSRPVVGILMGSDSDWQYIKPAASALHEFGVPFEVHVMSAHRTP
                     HQVAEYAAQAETRGLKVILAAAGGAAHLAGVVAAHTTLPVIGIPVPTGLAGGLDSLLS
                     MVQMPGDIPVATVGVGGGGARNAGLLAISILALSDETLAQRLKTFRQRLSEKVKSRDS
                     QLQTKLAEEFGGSGETVG"
     gene            2195..>2960
                     /gene="mtnA"
                     /locus_tag="PKI05_09265"
     CDS             2195..>2960
                     /gene="mtnA"
                     /locus_tag="PKI05_09265"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOA52436.1"
                     /translation="MNESKPFSTLEWIGDIDGYLKILDQTQLPGKLVYIDCQDEHQVW
                     HAIKSLQVRGAPAIGIAAAYGVCLGLKALASPTEDSLFDRLEAVTAYLASSRPTAVNL
                     FWALDRMKRHAAACRGIMSHQQIRQALLEEARAIHEEDRAICRGIGDWGSTLLSDGMG
                     ILTHCNAGALATSELGTALAAIYTAVASGKRLSVFAAETRPLWQGARLTTWELSMHGI
                     PVTLICDNMAAQVMREGKVQAVIVGADRIAANGDTAN"
BASE COUNT          620 a          784 c          899 g          657 t
ORIGIN      
        1 tatgggctat tttggcccgg aatctcaaaa ctcctcgcgt gaatcagctt ttccgcggaa
       61 aattgtttac cttgtcgtta acccaattct tgggtagtat cacgatttta gaccgaaaaa
      121 ccgtcgtcat atgggcatgg gcaagcgtgt ccggtgacag aacgacctgg gtagtatttc
      181 atagcacgtt cgcggatggc aggtgccgca taacaagcac agcatggacg gttggcctga
      241 agtcgtcata accggtgttg gtattgtcag tcctattggc atcggctgtg aggccttctg
      301 ggagtctctt tgcgcggggc gatcaggcat caccaaaatt gaggcccttc gagagcaagc
      361 ttgggcatcc agcattggcg gggttgttac caattttgat gctaaagacc gcgtccgtcc
      421 ccgaaaagcc ctcaaggtta tgagtcgcga catccaaatg ggattcgtcg cggcggatct
      481 ggcggtcgag atgtcgggga tcaagtcgag tggtgtggag cccgagcgac tgggcattgt
      541 ttttggcgcc gatctcatcc cgtgcgaatt ggacgagatt caaaacgcga ttcgccgctg
      601 tatcgtggat gggcactttg acttctccct gtggggccag tacgcgatgt ccgatcttta
      661 tcctctatgg ctcctaaaat atttgcccaa tatgccggcg tgtcatattg gcattgccca
      721 cgatgcgcgg gggccaaaca atacactcac gctcgccgag gtctcgggcc tggcctcgct
      781 gatcgaagcg gtgcgggtca tccagcgggg tgaagccgat gtcatgattg cgggcgggac
      841 tgggtcgagg atcaatcctt ccatttgggt ccgcgaaggg gcctacgagt tgtcaaaacg
      901 agttgatgat ccgcagcatg cctgccgtcc gttcgatgca gctcgcgacg gcatgatcca
      961 cggcgaaggg gctggtgcag tggtcctcga acgccgtgac catgccgtag cccgaggggc
     1021 caggatcctg gcgcaagttc tcggctacgc ctgtacgttt gagccgcgac ggcttcatca
     1081 ccctgtccag ggtacggcca tccggcaggc aattcggtta tcgttaagaa tggctgattt
     1141 ggcgcctcgt gacgtgggac acgtcaatgc ccacggagtt agcaccgtgg acgacgacag
     1201 ggccgaggcc aaggcgatcc acagcgaact cggtgatgtc cctgtgacgg cattaaagag
     1261 cttctttgga aatttggccg ccggaacagg catcgtggaa atggcgggca gtcttttggc
     1321 ctttgcggga aagaagatac ccttcaccct gaactacgaa acgcctgatc cagagtgtcc
     1381 gattgacgtc gtggctaagg cacccagacc gttgaaaatc cccacggccg tgatcctcaa
     1441 tcactcacgg atcggacagg cagtctcgtt agtgctcgcc gccggctgat cctgaaaaac
     1501 tgattggcct gcgattttga tccagaaccc gaaaaagact cagccaagca agtgaggaga
     1561 gtgaaaaact atgtcccgac cggttgttgg catccttatg ggtagcgaca gtgactggca
     1621 gtatatcaag ccagcggcga gtgctttgca cgaatttggg gttccctttg aagttcacgt
     1681 gatgagcgcg catcgcacgc cacaccaagt tgcggagtac gcagcccagg cagaaactcg
     1741 cggtctgaag gtcattctgg cggctgcggg gggagctgcc catctggccg gtgttgtcgc
     1801 ggctcatacg accttgccgg tcattgggat ccccgtgccc acgggcttgg cggggggatt
     1861 ggactcgctg ttgtccatgg tccagatgcc gggcgacatc ccggtggcaa ccgttggagt
     1921 aggtggcggt ggcgcacgca acgccggcct tctggcgatt tcaatcttgg ctctcagtga
     1981 cgagacattg gcccaacggc ttaagacctt caggcagcgg ctcagcgaaa aagtcaagtc
     2041 gcgggatagc cagttgcaaa cgaaacttgc agaagagttt ggtggtagcg gggaaaccgt
     2101 tggttaggtt ctcttcgcag agttcgaccg ccctgagccg tcaattaggt gcttcacctc
     2161 aatatattac cattttggtc agaagatctt ggcaatgaac gaatcgaaac cgttttccac
     2221 actcgagtgg attggcgata tcgatgggta tctgaagatt ctcgatcaga cacagttacc
     2281 cggcaagttg gtgtacatcg actgtcagga cgaacaccag gtctggcatg cgatcaagtc
     2341 tctgcaagtt cggggggccc cggccattgg gatcgccgcg gcttatgggg tatgtctcgg
     2401 attgaaagct ttggcctccc ccactgagga cagccttttc gaccgattag aggccgtcac
     2461 agcttacctt gcgtcttccc gaccaaccgc ggtcaattta ttctgggctt tagaccgcat
     2521 gaagcgccat gccgcggcct gccgaggaat catgtcgcat cagcagatac gccaagccct
     2581 tcttgaagag gctagggcca ttcacgagga agaccgggcg atttgccgcg gtatcggcga
     2641 ttgggggagc acacttctgt cggacggaat ggggattttg acccactgca acgccggtgc
     2701 gctggccaca tccgaactgg ggacggcctt ggccgccatc tatacggccg ttgcctcggg
     2761 aaaaaggttg tcggtgttcg cggctgaaac tcgtccgctg tggcagggtg cacggctgac
     2821 aacctgggaa ctgagcatgc atggcattcc cgtgacgctt atttgcgata acatggcggc
     2881 tcaggtcatg cgggagggaa aagtccaagc ggtcattgtt ggagccgacc gaatcgctgc
     2941 caatggtgat accgccaaca
//
LOCUS       DAOPTR010000457         2959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_26763_length_2959_cov_2.632231, whole genome shotgun sequence.
ACCESSION   DAOPTR010000457 DAOPTR010000000
VERSION     DAOPTR010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26763_length_2959_cov_2.632231"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..1406
                     /gene="wecB"
                     /locus_tag="PKI05_09270"
     CDS             288..1406
                     /gene="wecB"
                     /locus_tag="PKI05_09270"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOA52437.1"
                     /translation="MVRKLACVVGARPNFMKMAPILRALRSYPDIRPILIHTGQHYDP
                     QLSDVFFQDLEMPPPDIHLNVGSGSHAQQIARILEKMEEVLADGAPDGGRFSRIIVVG
                     DVNSTLAAALAAAKLEIPVAHVEAGLRSFDRSMPEEINRLVTDTLADMLFVSEPSGVE
                     NLRQEGHSNDQIHLVGNVMIDTLFALLPRAQGLDTLARLGLRPKEYAVVTLHRPANVD
                     QRETLGGLLKVLADVALQLPIVFPIHPRTQKRIESFGLQHLLDNHKSSRGLNVTPPLG
                     YLEFLSLTSQAKVVITDSGGLQEESTALGIPCLTVRPNTERPITVTEGTSTLIGNDPD
                     KLRTCLEEVLNGRYKTGRCPDLWDGHAAERIAAILAHN"
     gene            1713..2708
                     /locus_tag="PKI05_09275"
     CDS             1713..2708
                     /locus_tag="PKI05_09275"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HOA52438.1"
                     /translation="MNRLPLLGCVMCLCLQTATFESVAWQIPVSGPDPTVIAVDSNGA
                     LSYYAFVTGRGIPVLHSTDLKSWQPIGRVFEQAVPDWARREVPGATGIWAPDISYHEG
                     RYYLYYAVSTFGSQRSVIGLAVNRTLDRESPDYRWVDCGKVIESSPNGCDYNAIDPAL
                     FVDNDGRWYLFFGSFWSGIKAIELIPGEGKPKPDAKIVSIAGRPHHPTHAIEAPFVVF
                     REGFYYLFASWDRCCDGADSDYKVVVGRAKQVLGPYVDAQGKPMLDGGGTLILAGNDR
                     WRGPGHNCVLHTETGDWIIHHTYDMQNLDKHRILQVRPLSWTEDGWPRVGQPLSN"
     gene            2779..>2959
                     /locus_tag="PKI05_09280"
     CDS             2779..>2959
                     /locus_tag="PKI05_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52439.1"
                     /translation="MIARIVSVTIIVCALLTTGQGRAVGQALSGKPSVRNVILLVSDD
                     HRYDFLGFMAQSPSFL"
BASE COUNT          626 a          751 c          814 g          768 t
ORIGIN      
        1 tttcaagtgt tttaggtttt ttcggtttgt ccctcatagc gcgggccgat tcaatttcag
       61 tggcaagttt gtactaagaa taattgatac acttgataca aacgttattt gtgcatttga
      121 acactctgtc agctaagata gcaatagaag acggtacata gatcgcattt ttccgaggtg
      181 tgctaaagac gacgctttcc gtgcgaaaag gagtattcgc gagttttcga gcgaggtaaa
      241 cgtgcgttga gagtttttaa taaaaggcgt gacagggcca aattcctatg gtgcgaaagc
      301 tagcttgtgt tgtcggcgct aggcccaatt ttatgaagat ggccccgatt ctccgggcac
      361 tgcggagtta tccggacatt cggccaattc ttatccacac aggtcagcac tatgatccgc
      421 agctcagtga tgtgtttttc caagatttag agatgccgcc gccggatatt catctaaacg
      481 ttggttccgg gtcacatgcg cagcagattg cccgcatctt ggaaaagatg gaagaggtcc
      541 ttgctgacgg cgcgcctgac gggggccgct ttagccgtat catcgtggtg ggtgatgtca
      601 attccactct tgccgcggcc ctagcagcgg ccaagctgga aatccccgtt gcccatgtcg
      661 aggcggggct gcgaagcttt gaccgcagca tgcctgaaga aattaaccgt cttgtcaccg
      721 acacgttggc agacatgttg ttcgtttctg agccttctgg tgttgagaat cttcgccagg
      781 aagggcattc caatgatcag atccatctcg tgggaaacgt gatgatcgac acccttttcg
      841 cgctcttgcc tcgcgcccag gggctcgaca cgctcgcccg gttgggtttg cgtcccaaag
      901 agtatgcggt cgtgacgttg caccgcccgg caaatgtcga tcaacgtgaa acgctgggcg
      961 ggcttctaaa ggtcctggct gatgtggctt tacagctgcc gatagtcttt cctattcatc
     1021 cccgaacgca aaagcgcatt gagtctttcg gccttcaaca tttgctcgat aaccacaagt
     1081 cctcccgagg attgaacgtc acgccgccac taggctacct cgagtttctc tcgctgacat
     1141 ctcaagccaa ggtggtgatt accgactcgg gcgggcttca agaggaaagc accgcgctcg
     1201 gcattccctg cttaacggtg aggccaaata ccgagcgccc catcaccgtc acggaaggga
     1261 ctagcacact gataggaaac gatcctgata aacttcggac gtgtttggag gaggttttga
     1321 acggtcgcta caagaccggt cgctgtccag acttgtggga cggccatgcg gcggagcgga
     1381 ttgcggctat tctcgcccac aactaaggtt agccctatga gccaaacccg ctcaacatcc
     1441 ctcgccatca aaaccaggcg aaaaactgcg acgttgtctc tttcgcgact cgcctgacgt
     1501 tttctgtctt tcttttccat tttcgtctcc gtttcctgat tcattctgct atgcggacct
     1561 cgaaatgagg tccgtttttt tgttttacgc tgagtcttgc accgattgca ctaggcgatg
     1621 atgtgcggaa taatctattg gaaatggcac cttttttctg gggtcaccgt aatgcgacgg
     1681 ttatttcctg tgagcttgag gataaaaaga caatgaatcg tttgccccta ttaggctgcg
     1741 taatgtgcct atgtcttcag acggcgacct ttgagtccgt ggcctggcag attccggtgt
     1801 ctggaccgga tcccacggtg atcgctgtgg attccaatgg ggctttgtcc tattacgcct
     1861 ttgtcacggg gcgcggaatc ccggttcttc actcgaccga cttgaagtct tggcaaccaa
     1921 ttgggcgtgt ttttgagcaa gcagtcccgg actgggctcg gcgagaggtc cccggtgcta
     1981 cgggaatttg ggcccccgac ataagctatc atgaggggcg ttactatctg tattacgccg
     2041 tgtcaacatt tggaagccaa cgatctgtga tcggccttgc tgtcaacaga accctcgatc
     2101 gagaaagccc tgactatcgg tgggttgatt gtggtaaggt catcgagtcg tccccgaacg
     2161 gatgcgacta taatgccatc gaccccgcct tgtttgttga caatgacggt agatggtatc
     2221 tgtttttcgg ctcgttttgg tcggggatca aagccattga attgatccca ggcgagggca
     2281 aacccaagcc agacgcaaag attgtttcga tcgctggacg gccgcaccac cctacccacg
     2341 cgattgaagc tccgtttgtg gtgttccgtg aaggattcta ttaccttttc gcgtcctggg
     2401 atcgctgttg tgatggtgcc gatagcgact acaaggtggt tgtaggacgg gcgaagcagg
     2461 tgttggggcc ttatgtggat gcccagggaa aaccgatgct tgacggaggt ggaacactga
     2521 tcttggctgg aaacgaccgt tggcgtggcc cggggcacaa ttgcgtcctc cacaccgaga
     2581 caggcgactg gatcattcac catacctacg acatgcaaaa cctggataaa catcgaatct
     2641 tgcaagttcg gcctctttcg tggacggaag acggttggcc gcgtgtcggt cagcctcttt
     2701 caaactaaat taggcaggtt aggcgggctg ccttaggccc gtggtatttg tgcaacgcgt
     2761 tttcgtggag tcgttcccat gatagcgaga attgtcagcg tcaccatcat tgtttgcgct
     2821 ttattgacaa cggggcaggg acgagccgtt ggccaggcgc tatcaggaaa gccatctgtg
     2881 cgaaatgtga tccttttggt gagcgacgat catcgctacg actttttggg attcatggcc
     2941 cagtctccgt ctttcttgg
//
LOCUS       DAOPTR010000458         2958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_26775_length_2958_cov_3.012401, whole genome shotgun sequence.
ACCESSION   DAOPTR010000458 DAOPTR010000000
VERSION     DAOPTR010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26775_length_2958_cov_3.012401"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PKI05_09285"
     CDS             complement(<1..261)
                     /locus_tag="PKI05_09285"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOA52440.1"
                     /translation="MVNVVSNNRAIAAVELSSVGVGYEVEDAMLKSASVQLLIARTIC
                     SGKYLIIVGGLVSDVQAALQAGLEAARDSVIDQLLIPNVHESV"
     gene            complement(325..1662)
                     /locus_tag="PKI05_09290"
     CDS             complement(325..1662)
                     /locus_tag="PKI05_09290"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOA52441.1"
                     /translation="MVQVTVDQVFEAGVVGAGGAGFPTYVKLSGRADTVLINAAECEP
                     LLHKDKEILRHFDDDVLAGLRIAMELVGASRGVIGIKGKYADVIEQLRPKLSPDMEIV
                     PLPDAYPSGDEFILVYEALGRVIPPGGIPLHVGAVVINVETARNVAMASRQPVVEKFL
                     TIAGAVQEPVTVRVPIGVTLAECVELAGGPTVSNPQYMVGGVMMGYLEQNHHALVDKT
                     TGGVIVLPEDHVVIRRRQQDWKQISRIGRAACDQCSFCTELCPRWLLGHPIEPHRAMR
                     SLGFNLVGEANVQGTVFCCECNLCSLYSCPEDLDPKNVCVQNKRRILAEKRRWENPPF
                     LPERAERLLKNRRAPTKRLMKKLGLTLFPNVGYLVERTVTPKMVGIKLKQHVGVPCEP
                     VVRVGDKVVRGQVVGMPPETDGKRALGAPVHASIDGVVTRIQDGVVWIERQAS"
     gene            2325..>2958
                     /locus_tag="PKI05_09295"
     CDS             2325..>2958
                     /locus_tag="PKI05_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52442.1"
                     /translation="MPKYALILVCVILGVFADTSFSQVCNIKVVTDANPDYYDLDSLI
                     HSATGAWKTPEEKCWALFYWVHISRRQTSPMIFHGVEVTDPIRQFNDFGYTMCSTVAG
                     INCGLWHHMGFPVRFWDVTLHTVSECFYNERWHMYDNSMSAIYTLCDGKTVAGVEDLG
                     REGACEASEGRYETGHIVKYHCLTATSPNGFLTGADCARDLDQEARCFNPA"
BASE COUNT          678 a          853 c          750 g          677 t
ORIGIN      
        1 cacggactca tggacattcg ggatgagcag ttggtcaatg accgaatccc tagctgcttc
       61 caagcccgcc tgtaaagccg cctgaacgtc gcttactaac ccaccaacga tgatgaggta
      121 ttttcccgag cagatcgttc gcgcaatgag gagctgaacg gacgccgatt tgagcatggc
      181 atcttccacc tcgtagccga cgcctacact ggacagctca actgccgcga tcgctcgatt
      241 gttggaaacc acattaacca taagcaccac ctagattgag ttaatgactt cctgcgaaac
      301 cagaatccgc tagttaacgc tcactcacga ggcctgtcgc tcgatccaca ccacaccatc
      361 ctgaattcgt gtcaccacac catcaatcga tgcatggacg ggtgcaccaa gcgcccgttt
      421 accgtcggtc tctgggggca ttcccaccac ctgaccgcga acgaccttgt ctcccaccct
      481 tacaaccggc tcgcacggca cgcccacgtg ttgtttaagc ttgatgccga ccatcttcgg
      541 ggtaacggtc ctttctacca ggtaacccac atttgggaac aacgtaagcc cgagcttttt
      601 catcaatcgc ttggtcgggg ccctgcgatt ttttaggagg cgttcagcgc gctctggaag
      661 aaagggagga ttctcccagc ggcgtttttc ggcgaggatg cggcgcttgt tttggacaca
      721 aacattcttg ggatcaaggt cttccggaca agaatatagg ctacaaagat tgcactcaca
      781 gcagaacacc gtcccttgca cattggcttc gccaaccaga ttaaatccga ggcttctcat
      841 cgcacgatgt ggctcaatgg gatgacccaa gagccatcgc ggacataatt ccgtacagaa
      901 actgcactga tcacaggcgg cccggccaat ccgcgaaatc tgtttccaat cctgttgcct
      961 tcgccgtatc accacgtgat cttccggcag cacgatcacg ccgccggtgg ttttatctac
     1021 caacgcgtgg tgattttgtt ctaggtaacc catcatcacg ccgcccacca tatactgcgg
     1081 gttggagacg gtcggacctc cggcaagctc aacgcactcc gccaaggtca cgccaatagg
     1141 tacacggacc gtcacaggtt cttggacggc cccagcgatc gtcaggaatt tttccaccac
     1201 aggctgacga ctagccattg ccacgttccg agccgtctcc acattgataa ccacggcccc
     1261 gacgtgaagg gggataccac ccgggggaat cacccgacct agggcctcat agaccaagat
     1321 aaactcatcg ccggaaggat atgcatcagg taggggcacg atttccatat ccggggacag
     1381 cttcggccgg agctgctcga tgacatcggc gtacttgccc ttaatcccaa tgacgcctcg
     1441 cgatgccccc actaactcca tggcgattcg caaaccagcc aagacatcgt cgtcaaaatg
     1501 acgcaggatc tccttgtcct tatgtaaaag tggttcacac tctgccgcgt taatcaaaac
     1561 agtatcggcg cgacctgata atttgacata cgtcggaaag ccggcaccac ccgcaccgac
     1621 caccccggct tcgaaaacct gatcgacggt aacttgaacc attaccaaaa tcccttacat
     1681 caagtcctgg caaaaaagac gcgaagcgtg ctggaccgcg gtcgcctgtc cattggacgc
     1741 cccttcaaat gcctgaatat gaccggcaaa gtctatcttc tcgacaatca tagctcatat
     1801 cacgtgtgtt ctaaagtcca tcagagcgag cgcattttac cgcaaacgcc gtaaccaccg
     1861 gcgccgatac tcaatgtttg tattttcgaa ggaccttttc ccagattgag atgggttgtt
     1921 gccattcaag gagttgagga gtatcccaat gtgctgatgc ccaaggagtg gtcgagtcag
     1981 agatgcgaca gatctgcgtg ccaccgtgtt atggcggcca tgacggcccg ggcttctcat
     2041 taccgcgggc aggcccaagg cctatctctg ccgtttgcgg acgaccttcc cagtaggggc
     2101 aacccttgcc gttacccttt gtcgaatctg gcggtccgtc aggtatctgg accaacgcgt
     2161 cgtcccgtcg gttgcggttg tgttttggga cgcagcggtt tatgccagac ggaaacagta
     2221 taaaccggtt caaactgtga acaaaaactt gacggtgttg caggcgatgc tgacaatggc
     2281 tctcgtctgc gacgtcgtat tcagtcgtat tgaggagagt cgctatgcca aagtatgcct
     2341 tgatcctcgt ttgtgtgatt cttggggttt ttgccgatac ttcgttctct caggtctgta
     2401 acatcaaggt ggtaaccgat gctaatccag actactacga cctcgacagt ctgatccaca
     2461 gtgccaccgg cgcttggaaa acgccggagg agaaatgctg ggcattgttt tactgggtgc
     2521 atatttcgcg ccgtcagacg tcacccatga tctttcacgg cgtggaagtt accgatccca
     2581 ttcgtcagtt caatgacttt ggctacacca tgtgtagtac ggtggcgggc atcaattgtg
     2641 gattgtggca ccacatggga tttcccgtgc gattttggga tgtcacgctc cacactgttt
     2701 ccgaatgctt ctacaacgag cgttggcaca tgtacgacaa ttccatgtcg gccatctaca
     2761 cgctctgcga tggaaagacc gtggcagggg tcgaggatct gggcagggaa ggggcctgcg
     2821 aggcttcgga gggtcgttat gaaaccggcc atattgtcaa gtaccattgc ctcaccgcaa
     2881 ccagtcctaa tggcttttta acaggggcag actgtgcccg agatttggat caggaggcac
     2941 gctgttttaa tcccgcgg
//
LOCUS       DAOPTR010000459         2957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_26788_length_2957_cov_4.813232, whole genome shotgun sequence.
ACCESSION   DAOPTR010000459 DAOPTR010000000
VERSION     DAOPTR010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26788_length_2957_cov_4.813232"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..2890)
                     /locus_tag="PKI05_09300"
     CDS             complement(44..2890)
                     /locus_tag="PKI05_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOA52443.1"
                     /translation="MKTLAIDIETYCEAELDKTGQYAYAQDPTFEILLFAYAFDDDDV
                     RVVDLAQGETIPDEVLRALQDPDVLKTAWNAAFERTCLSVYLGTPMPADQWACTMVHS
                     YYVGLPGSLEKAARSLKLGVQKDSAGAALIRYFSKPCRATKSNGGRTRNLPEHDLERW
                     EQFKAYCRQDVEVERTIRKRLETVPVPDKERRLWAVDQKINDRGVAIDKDVVRQAIKL
                     NETLQERLIAEAEILTGLDNPNSVSQLKNWLQQAEGLEIESLNKKAIPKVIAQAESAV
                     TQRVLEIRQQLAKTSIKKYQAMERALCPDGRIRGLLQFYGANRTGRWAGRLVQVQNLP
                     RNSMPDLHTARELLKAGHLEAIELLWDDPADVLSQLIRTAIIPSPGRQFIVADFSAIE
                     ARVVAWLAGEQWRLDVFRDHGKIYEASASQMFGVPIEKIDKGSELRQKGKIAELALGY
                     GGGVGALKAMGALEMGIEEEELQELVQRWRAANPAIVQLWRDLGQAAIEAVRDQNIVT
                     LQHGITFWVEGGILRVKLPSGRVLHYVRPRIEQDPKFGTDIVTYEGYEQGRWGRLKTY
                     GGKLTENCLAGDTLVLTTRGWVPIKDVRPTDLLWDGVAWVRHQGVVEKGKQPTLTLDG
                     VRLTADHLVLTTGGWRNASSCKGYYRQAVTLPHCGSVRRFRRQEVTLASPLRLRQDAD
                     HACYRVPKGQVEVVRLQKTGTHKRIAEDTRHVETPGLCCVAINDRPLSPPDPPCLEEL
                     RRSGNSSLQSLANVRGVLGRHGAVVQTGADPRSDRRQRQLHSGQLPLGRHQGTKPQST
                     EQPLHPNALGADDRLGGVREVGHRGDDPAVSPQRGGSYGPFIPAPGRYEPVYDIVNAG
                     PRQRFVVLGDSGPFIVHNCTQAVARDCLAEAILRLDAAGYDIAFHVHDEVVIDAHPNT
                     DPEKVAAIMSAPIGWAPDLPLAADAFEAEYYRKD"
BASE COUNT          547 a          905 c          828 g          677 t
ORIGIN      
        1 aacaccttaa acggatcaat gggcgtagtc ctctgcacga tcatcaatcc tttctgtaat
       61 actccgcttc gaaggcatca gctgccaacg gcaggtccgg cgcccacccg atgggggcgc
      121 tcatgatcgc cgccaccttt tctgggtcgg tgttgggatg ggcgtcgatc accacctcgt
      181 catgaacatg gaaagcgatg tcgtagccag cggcgtccaa ccgcaggatc gcttcggcga
      241 gacagtctcg ggctacggcc tgggtacagt tgtgaacgat gaagggcccg gagtccccca
      301 gtacaacgaa gcgctgacga gggcccgcat tcacgatgtc gtaaaccggt tcgtaacgtc
      361 cgggggctgg aataaaaggt ccataggaac ccccgcgttg aggcgataca gcagggtcgt
      421 caccccgatg cccgacctct ctgacgcctc cgagacggtc atccgccccc aaggcgtttg
      481 gatgtagcgg ttgttccgtc gattgcggtt ttgttccttg gtggcgaccc aacggcaatt
      541 gtccggagtg tagttgccgt tgacgtcgat ccgatcgagg gtcagccccg gtctgtacga
      601 cggccccatg tcggcccaaa acccctcgaa cgtttgccag gctttgcaga cttgaattcc
      661 ccgaccgccg tagttcttcc aggcatggtg ggtcgggagg tgacagcggt cgatcattga
      721 tcgccacaca gcataggccg ggtgtttcga catgccgtgt gtcctccgcg atgcgcttat
      781 gagttcccgt cttttgcagc cgcacgactt ccacttgccc tttaggaact cggtagcagg
      841 catgatcagc gtcttgccgc aatcgcaacg gactcgccaa agtgacttct tgccgtctga
      901 acctgcgtac gcttccgcag tgaggaagtg tgaccgcttg ccggtaatat cccttgcacg
      961 atgatgcatt tctccaacct ccagtagtca agaccaagtg gtctgctgta agtctgactc
     1021 catcgagtgt cagagtaggt tgcttgccct tctcaacaac gccttggtgt ctgacccaag
     1081 cgacgccatc ccacagtaga tcagttgggc ggacatcctt tatggggacc cacccacgcg
     1141 tcgtaaggac gagcgtgtca ccggcgagac aattctcagt gagtttcccg ccgtaggttt
     1201 tgagtcggcc ccaacggcct tgttcgtagc cctcatacgt cacaatgtct gtcccgaact
     1261 tagggtcctg ctcaatccgt ggtctcacgt agtgcagcac ccgcccgctg gggagcttca
     1321 cgcgcaaaat gccgccttcg acccaaaaag taatcccgtg ctgcagtgtc acgatatttt
     1381 ggtcacgcac cgcctcaatt gccgcctgcc ccaggtcccg ccacagctgc acgatcgcag
     1441 ggtttgccgc ccgccatctc tggacgagct cttgcagctc ctcctcctct atccccattt
     1501 caagcgcccc catggctttt agcgcaccta cgccgccgcc gtaacccaac gcgagctcgg
     1561 cgattttccc tttctgccgt aactcgctac ccttgtcgat cttttcgata ggcaccccga
     1621 acatttggct tgcgctagcc tcataaattt taccgtggtc gcgaaaaacg tcgaggcgcc
     1681 actgttcacc ggcaagccat gccaccactc tggcttcgat ggctgagaag tcggccacaa
     1741 tgaattggcg cccgggcgag gggataatgg cggtgcggat gagctgagag agcacatccg
     1801 caggatcgtc ccaaaggagc tcgatagcct caaggtggcc ggctttaagg agctctcttg
     1861 cggtgtgtag atcgggcatg gaatttctcg gcaggttctg gacctgcacg agacgcccgg
     1921 cccaacgccc ggttcggttg gcgccgtaga actggagcag cccccggatc cgcccgtctg
     1981 ggcatagcgc ccgttccatg gcttggtatt ttttgatcga tgtcttggcg agctgctgtc
     2041 taatctcaag cacccgctga gtgacggcgc tttcggcctg cgctatgacc ttcgggatag
     2101 ccttcttgtt gaggctctca atttcgagcc cttcggcctg ttgaagccaa ttttttagct
     2161 gggagacgct gttagggtta tcgagcccgg tcaggatttc agcctcggcg atcaaccgct
     2221 cttggagtgt ttcgttgagc ttaattgcct gtctgacgac atctttgtcg atggcgacgc
     2281 cccggtcatt gatcttctgg tcgacggccc acagccgccg ttctttatcc ggcaccggaa
     2341 cggtctcaag tctcttccgg atcgtccgct cgacctcgac atcttggcgg cagtaagcct
     2401 taaattgctc ccagcgctcc aggtcatgct ccggcaagtt gcgtgtccgg ccgccgttgc
     2461 ttttggtcgc ccggcacggc tttgaaaaat agcggatcag cgccgcaccg gcagagtcct
     2521 tttgcacgcc aagtttcaaa ctccgtgcgg ctttttcgag actgccgggt aatccgacat
     2581 agtaggagtg caccatcgtg cacgcccatt ggtcggctgg catgggcgtg ccgagataga
     2641 cgctcagaca cgtccgctcg aaagcggcgt tccaggcggt cttgagtacg tcggggtcct
     2701 gcagcgcacg gagcacctcg tcggggatgg tctcgccttg cgctaggtcc acgacccgca
     2761 cgtcgtcgtc gtcaaaagcg taggcgaaaa gcagaatttc aaaggtaggg tcttgagcgt
     2821 aggcgtattg ccccgtctta tccaactctg cctcacagta ggtctcgatg tcaatcgcca
     2881 gtgttttcac ggtgatacca ccccctaggc cgcggcacac tatgtggaga cgtcataggg
     2941 ccgcggcctg agttttt
//
LOCUS       DAOPTR010000460         2932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_27073_length_2932_cov_2.779284, whole genome shotgun sequence.
ACCESSION   DAOPTR010000460 DAOPTR010000000
VERSION     DAOPTR010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27073_length_2932_cov_2.779284"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PKI05_09305"
     CDS             <1..230
                     /locus_tag="PKI05_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52444.1"
                     /translation="RRRSIARRDDKKPAEDREGSQKEKFAAEKATDHPPGNRGFRKAV
                     RNPKKPFPGWTWVVRGGGYPKSRKQAGRAGE"
     gene            392..625
                     /locus_tag="PKI05_09310"
     CDS             392..625
                     /locus_tag="PKI05_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52445.1"
                     /translation="MLGTAATALGQTKGVKGQLLKGRAETGSGFIDPRAGYGGRTWGI
                     RERPHGGELTLAPIAQVVRFSGPLIASLPEHLQ"
     gene            747..2036
                     /locus_tag="PKI05_09315"
     CDS             747..2036
                     /locus_tag="PKI05_09315"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOA52446.1"
                     /translation="MSFDYIVSQKLAIDGGPRAVTNKLPGWPQFSEEAIRAVEAVLRS
                     GRVNYWTGPHGMAFEKRFAEWQGSRFAIAVSSGTAALHVALSALGIGPGDEVIVPSYT
                     FIATSFAVLQAGAIPRFADVNLEDHCISVESAEKLVNERTRAIIPVHLYGNVCDMDKI
                     NDFAKRHNLFVIEDNAEAFGGEYKGKKTGTLSHIAACSFCQNKTFTTGGEGGMVTTDD
                     EDLAWEARSFRDHGYDVKERLNLLELEQKLPYIHTRLGWNYRMTEMQAVIGLAELDRM
                     DTWNMPRRRRNAHILMDALMPLPQIKFGPIDTEERRNGFYVCAFSLDIENMGCDIQQF
                     VKAATAEGAPVWKVFWPQCHTERAYRERRGFGRTGFPFTSKEYTNPESVDYSNVEVPN
                     ALWHETHTFTCFAFPTCSEEDMQQIAAALVKVIKAYS"
     gene            2111..>2932
                     /locus_tag="PKI05_09320"
     CDS             2111..>2932
                     /locus_tag="PKI05_09320"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA52447.1"
                     /translation="MGEKVRFGVIGAGGIAFRKTIPAMLRARNLRVVAVMDTANVEKV
                     AEAFRIRRAYTTAEDLLADPEVDAVYIASPVHCHLEQIELAAAARKHILCEKPLTLDV
                     AQTRRAIDVCRRKKVFLQEGYMMKFHGAHQVIRENISQGRLGKVVYLRAQLSCWYPPI
                     PGAWRQDPKTGGGGALIDMATHLLNLLEFFVGPIRRLAALTGNLVHPYRSEDASTILA
                     EFRSGAHATIDCFWCIPDEASRTRLEIYGSAGAIFTEGTIGQSVGGTMEGIFGLGS"
BASE COUNT          638 a          739 c          940 g          615 t
ORIGIN      
        1 ctcgccgcag gtccattgcc agacgggatg ataaaaaacc ggcggaagac cgtgaaggaa
       61 gtcaaaaaga aaagtttgcg gcagaaaaag ccacggacca tcctcccgga aatcggggtt
      121 tccgaaaggc ggttcgcaat ccgaaaaaac cgtttccagg ttggacatgg gttgtccggg
      181 gtggtggata tcctaagagt aggaagcaag cagggcgggc tggtgagtga tgccagcgag
      241 gtgggtttcc gcagaagggg caggaaaggc ggggccggga cagagaaggg gcaagtgggc
      301 cgtgcgccgg cgtgccgaga ggggtgatgt gtggaggaaa gagggctttc ggggcaggct
      361 ttgaaaaagg ggtaaatagc gaagggctgc gttgttaggc actgccgcca cggccctggg
      421 gcagactaag ggggtgaagg ggcaacttct taaggggcga gccgaaacgg gcagcgggtt
      481 tattgacccg agggcaggct acgggggcag gacctgggga atcagggaac ggcctcatgg
      541 gggcgagtta actctcgccc ccatcgccca ggttgtgcgc ttttctggac ctcttatcgc
      601 ttcgcttccg gaacacttgc aatgaccatc tctgtgggct aaaacatctc ttgccgttgg
      661 ctagtcgtga ggcagggagg accgatggaa gaaagcgaca gggtcgtttc gggaagtggt
      721 cttgtgctaa cgcatgaggt gctgtgatgt cgttcgatta cattgtctca caaaagttgg
      781 cgattgatgg tggtccgcgg gcggtgacga acaagctccc tggttggcca cagttctcag
      841 aagaagccat tcgcgccgta gaggcggtcc tccgatccgg gcgcgtcaat tactggacgg
      901 gtccccatgg tatggccttt gaaaagcgct tcgcagaatg gcagggaagt cgctttgcca
      961 tcgccgtttc ctccgggacc gccgccttgc acgtggcctt gtccgccctt ggcattgggc
     1021 caggtgatga ggtcatcgtt cccagttaca cctttatcgc caccagtttt gccgtcttac
     1081 aggccggggc catcccacga ttcgccgatg tcaacctcga agatcactgc atcagcgtgg
     1141 aatcggcaga aaagcttgtc aacgagcgga cgcgggcgat cattcccgtc catttgtacg
     1201 gcaatgtgtg cgacatggat aagatcaacg attttgccaa acgacacaat ctgttcgtga
     1261 tcgaagacaa tgccgaggcc tttgggggcg agtacaaggg caaaaagacg gggactttga
     1321 gccacatcgc ggcttgtagc ttctgccaaa acaagacctt taccacaggc ggcgaagggg
     1381 ggatggtaac gacggatgac gaagacctgg cctgggaggc tcgcagtttt cgggatcacg
     1441 gttacgacgt gaaagagcgt ctcaatctcc tggagctgga gcaaaaactc ccttacatcc
     1501 acacgcgatt gggctggaat tatcgcatga cggagatgca ggccgtcatc ggattggccg
     1561 agctggatcg gatggatact tggaacatgc cgcggcgacg tcgcaacgcc cacatcctca
     1621 tggatgccct catgccgcta ccgcagatca agtttggtcc catcgacacg gaagaaaggc
     1681 ggaacggctt ttacgtgtgt gccttctcgt tggacattga gaatatgggg tgcgacatcc
     1741 agcagtttgt gaaggcggca acggcggaag gtgcccccgt gtggaaggtc ttttggcccc
     1801 agtgtcacac agaacgggca taccgtgagc ggcggggctt cgggcgaacc ggttttccgt
     1861 ttacatcaaa ggaatatacc aaccccgaga gcgtcgatta ctccaacgtc gaggtcccca
     1921 acgccttgtg gcacgaaacc cataccttca cctgttttgc ctttcccacg tgttccgagg
     1981 aagatatgca acagattgcg gcggccttgg tgaaggtgat caaggcgtac agctagcgat
     2041 ccaagggcta ggacgggctt ttctgggcga gttgaatcaa aacatcgttt tagaaaagag
     2101 gagccgtatc atgggcgaga aggtccgttt tggggtcatc ggcgccggtg ggattgcttt
     2161 tcgcaagacg attcctgcca tgctccgcgc gcggaatctg cgggtggtgg ccgtgatgga
     2221 tacggcgaac gtcgagaagg tggccgaggc gtttcgcatt cgtcgagcct acactacagc
     2281 cgaggatttg ttggctgatc ccgaggtgga tgctgtgtac attgcctctc ccgtacactg
     2341 ccacctggag cagattgagc tggcagcagc cgcccgtaag cacatcttgt gtgaaaagcc
     2401 gctcaccctg gatgtcgcgc agacacgaag ggccattgat gtctgccgac gaaaaaaggt
     2461 cttccttcaa gaaggctata tgatgaagtt ccacggagct catcaggtca ttcgggaaaa
     2521 tatttcgcag gggcggttgg gaaaggtcgt ttacctgcgg gcacaactgt cctgttggta
     2581 tccgccgatc cccggggcat ggcggcagga ccccaaaacc ggcgggggtg gagccttgat
     2641 tgatatggcg acgcatctgc ttaatttgct ggagttcttt gtcggaccga ttcgtcggtt
     2701 ggcggccctc accggaaact tagtacaccc ctaccgctcc gaggacgcct caaccatttt
     2761 ggcggagttt cgctccgggg cgcacgctac cattgattgt ttttggtgta ttcccgacga
     2821 ggccagccga acacggttgg agatttacgg ctcggccggg gccatcttta cagaaggtac
     2881 gattggtcaa agtgtcgggg gaacgatgga gggcatcttc ggcctcggtt cg
//
LOCUS       DAOPTR010000461         2928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_27125_length_2928_cov_3.134702, whole genome shotgun sequence.
ACCESSION   DAOPTR010000461 DAOPTR010000000
VERSION     DAOPTR010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27125_length_2928_cov_3.134702"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PKI05_09325"
     CDS             <1..256
                     /locus_tag="PKI05_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52448.1"
                     /translation="DVPQSLRRYGDMYAEWIGGIKNGNPDQPSCPFSYAGPLTEAYLL
                     GNIALKLGRTIQWDAKARKITNIPEANQLLKSDYRQGWGA"
     gene            391..1827
                     /locus_tag="PKI05_09330"
     CDS             391..1827
                     /locus_tag="PKI05_09330"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOA52449.1"
                     /translation="MRACLASIVVLSCVSFGLTQGAEPIALHPDNPHYFLWRGRPTVL
                     ITSGEHYGAVLNADFDFIRYLDALAKDGLNHTRTFSGTYREVPSSFGITDNPLAPKPF
                     RYVCPWARSDQPGYYDGGNRFDLKKWNPEYFSRLHAFMQAARDRGIVVEFNLFCPFYN
                     DELWAANPMNVKNNVNGIGACPREEVYTLKHADLTEVQLDFVRKVVSELREYDNLYYE
                     VCNEPYFGGVTEAWQWKIADTIVETEKTWGVRHLISMNIANGRAKVEKLHPAVKILNF
                     HYCVPPDTVALNYHWNVVIGENETGFRGKDDILYRSEGWDFILAGGALYNNLDYSFTP
                     AHPDGTFLDYKSPGGGSPTLRRQLSVLKKFVEALPFWQMKPDSGTIKGVEPDLTASCL
                     SKKGAVYAIYLHVPLPNKPKNLAEHLRKNVTAKVALDLPTGRWRVRWLDTKSGAWVLD
                     ESRQHDGGLLTLTSPVFDDDIALRIDRD"
     gene            complement(1903..>2928)
                     /locus_tag="PKI05_09335"
     CDS             complement(1903..>2928)
                     /locus_tag="PKI05_09335"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOA52450.1"
                     /translation="FSAASVLIGLTLVTVAAPGKAMSVEKQQAIEARFGRRELPREDQ
                     VKRVPDIVLDLIPENPLQEAVGALDGSSKGNGMLAIMVFALVLGAATIKAETRGEPWI
                     KVLDSLYAVSMIIVDWAMTLAPFGVACLMFVAGARLGIALVVPLLGFLLVVVGGLLLQ
                     MFVVYPAALWISGVRPSQFFRNLGPVIPTAFGTASSSVTLPTAIQVAERNLLIPSHIA
                     RFILTVGATGNQNGTALYEGVVVLFLAQVFGADLTIWQQLGVVLFAILAGIGTAGIPG
                     GSIPMIMIILASIGVPPESVALILGLDRFLDMCRTVVNVTGDLTIAQCVAAWSGHVPK
                     GTDDGTT"
BASE COUNT          690 a          791 c          801 g          646 t
ORIGIN      
        1 tgatgtgcca caatcgctgc gtcgctacgg tgatatgtac gcagaatgga tcggcggaat
       61 caagaacggc aaccccgatc agcccagttg tccgttcagt tatgccggcc cattgacgga
      121 ggcgtatctg ttggggaata ttgccctcaa actagggcga acgatccaat gggatgcgaa
      181 agcccgaaaa atcacgaata tccctgaagc gaaccaattg ctgaagagtg attatcggca
      241 gggttggggc gcctaaacat aaccttcgtc cccgaagtgg cttgggttat cgagcatacg
      301 ctgggtttgc gacaaatgtt tcgtgatgac tggcagttca gaattgccag ttacggtgct
      361 agtttcggcc ttataaaggg aaccgccatt atgagagctt gcctggcctc tatcgttgtt
      421 ctatcttgtg tatcctttgg actaacgcag ggtgccgagc cgatcgcttt gcatccagac
      481 aatccgcact attttctttg gcgaggtcgg ccgactgtcc tcattacgtc tggcgagcac
      541 tacggggccg tcctcaatgc tgatttcgac ttcattcggt atctagatgc cctggcaaaa
      601 gacgggttga atcatacccg taccttttcg ggtacctacc gagaggttcc ctcttccttt
      661 ggaatcaccg acaatccgct ggcgccaaag ccgtttcggt atgtctgtcc ctgggcgagg
      721 agtgaccagc caggctatta tgacggcggc aatcggtttg acttgaaaaa atggaatccc
      781 gaatactttt cgcgactgca cgccttcatg caggcggccc gggaccgtgg gatcgttgtc
      841 gaattcaacc tgttttgccc cttctacaac gacgagcttt gggctgccaa tcccatgaac
      901 gtgaaaaaca atgtgaacgg catcggtgcc tgcccacggg aagaggttta tacactcaag
      961 cacgctgatc tgactgaggt gcaactggac tttgtgcgga aagtggtttc cgagctgcgg
     1021 gagtacgaca acctctatta tgaggtgtgc aatgaaccat actttggcgg tgtgaccgag
     1081 gcgtggcagt ggaaaattgc tgatacgatc gtcgaaacag aaaaaacgtg gggcgtccga
     1141 catctgattt cgatgaatat tgcgaatggc cgtgccaaag tcgaaaagct ccatccggcc
     1201 gttaagattc tcaattttca ctattgtgtg ccgccggaca ccgttgcgct caactatcac
     1261 tggaacgtgg tcatcgggga aaacgagacg ggattccggg ggaaagatga tattctgtac
     1321 cggagcgaag gatgggattt tatcctggcc ggaggtgcac tctacaacaa tttggattat
     1381 tcgtttaccc cggctcaccc cgacggaacg tttctcgatt acaaatctcc tggtggtggt
     1441 agtcccactc ttcgacggca attgtcggtt ctgaagaagt tcgtcgaagc attgcctttt
     1501 tggcagatga agccggactc gggaacaatc aagggcgtgg aaccggacct aaccgcaagt
     1561 tgcttgtcga aaaagggggc tgtctatgcc atctatcttc atgttccgct tcccaacaaa
     1621 cccaagaatc tggcggaaca ccttcgcaag aatgtcacgg caaaggttgc actggatctt
     1681 ccgacgggac gttggagagt tcgctggctt gacaccaaga gcggggcatg ggtccttgac
     1741 gaatcccggc agcatgatgg aggattgttg accctgacct ccccggtctt cgacgacgac
     1801 atcgcactgc ggatcgatag ggattgagct cttcgagttg actatcacgc cttggaccac
     1861 ggttgtccgg ccccatttcg tcgcaagcgg ggcccggcga ggctaggttg tgccgtcgtc
     1921 cgttcctttt ggaacgtgac cgctccaggc tgccacgcac tgcgcgatgg tcaaatcacc
     1981 cgtgacgttg accacagtcc gacacatgtc caggaagcgg tccagtccca agatgagggc
     2041 cacgctttcc ggcggcacac cgatgctcgc caggattatc atgatcatgg ggatggaacc
     2101 gccgggaatg cctgcggttc cgatgccggc caaaatggca aacagaacga cgcccaattg
     2161 ttgccatatt gtcaagtctg caccaaacac ttgagcgaga aagaggacga ccactccctc
     2221 gtacaaagct gtgccgtttt ggttgccggt tgcaccgacc gtgagaataa accgggcaat
     2281 atgggatgga atgaggaggt ttctctccgc cacctggatg gccgtcggta gcgtgacgct
     2341 cgatgacgcc gtaccaaagg ccgtcggtat gacgggccct agattgcgga agaactggct
     2401 cggacggaca ccactgatcc acagtgcggc gggatagacg acaaacattt gcaggagcag
     2461 cccgcccacc actacaagga ggaaacccaa caacggtacc acaagggcaa ttcctaatcg
     2521 ggcccctgcc acaaacatca agcatgctac gccaaaaggt gccaatgtca tcgcccaatc
     2581 gacgatgatc atggaaacag catagaggct atccaggacc ttgatccagg gctcaccccg
     2641 cgtttccgcc ttgattgttg ctgccccaag gacgagtgca aagaccataa tcgcgagcat
     2701 accattacct ttggaagacc catccagggc tcccacagcc tcttgaagag gattttccgg
     2761 gataagatca agcacgatat ccggcacacg ttttacttgg tcttcacgcg gcaattcccg
     2821 tcgcccaaaa cgagcctcaa tggcctgttg cttctccacg gacatcgcct tgcccggtgc
     2881 cgcgaccgtg acaagggtta aaccgatcag gacagaagcc gccgagaa
//
LOCUS       DAOPTR010000462         2927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_27135_length_2927_cov_3.211699, whole genome shotgun sequence.
ACCESSION   DAOPTR010000462 DAOPTR010000000
VERSION     DAOPTR010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27135_length_2927_cov_3.211699"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1366)
                     /locus_tag="PKI05_09340"
     CDS             complement(<1..1366)
                     /locus_tag="PKI05_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52451.1"
                     /translation="MIFRKSDFPVCRVMPSFGRTLLSVVLFSLIRHGGNLPFLERPIF
                     AESPPTGIDNPCTNGSFEVLGPNGFPVDWGPVGREVSVSEDAHTGQRSLRLVRSADTP
                     TPETGLNRTKLISELKGGVDFWYKAVSAKGAKLNVYVIPVNDQGIERTGSPRATFTVP
                     EDHIGDGQWHHARLKYDYSDNPKVKSVHFAARIVGTSGEILLDDIQYVAEVGPVPHIK
                     KVMLEENSEAPGQRGTIRVLVTNLGDEPAKDVKVTVTLPGKTTFRPVEMQLPILRPDE
                     IKPLYCPLAVVRNEPLTVHAKVVAGELEDTRRLELTPALRVRSFGPTMPVVTVGQTVV
                     LECELENIGAITLSGVLVTFETPRGASRQVVKSLAPTRRTVVRHTFSVDAETMQLPVR
                     VIVQSQGIAIAEGSDGQWTAESRIVVVKAISPPTPVGRFSVVPRPIPILENEHFRLVS
                     YPCEQ"
     gene            complement(1412..>2927)
                     /locus_tag="PKI05_09345"
     CDS             complement(1412..>2927)
                     /locus_tag="PKI05_09345"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOA52452.1"
                     /translation="LADAFVDYIARCSFLLQQGEPVTDVCYYYGDRGFNFVMEKSIDP
                     ELGPGYDYTVTSSEALLTRAAVKNGRIVFAPDQEGVALLVLPPGSELTPEVAEKLESL
                     IREGAIVVGTKPQHSPSLTGFPSCDEKVKAIGDRIWGENPPPKGERSYGKGKVFWGQP
                     LREVLQRLEIPPAVVCDAAADASVDFVHRRTASAEIYFFWNRLPRWEHLLVRLRAKEK
                     LPEIWNPVSGQMTPALAYRKTQQGLEMPLDLPPRGSLFVVLSDTTAAQSAGPGLDPVV
                     SVTRDGRELVFAPASGEAPVSVQPNGQGLPLLSAAESGTYKLRFQSGRELAISVEAPQ
                     VLQVPGPWQVEFPPGWGAPERITLERLISWTDHPNPGVKYFSGVATYRTTFRYEPVSE
                     PRRVVLNLGEIRFVGEIALNGKVLGILWTPPYELDVTGVIKEGENELVVRVANVWSNR
                     LTGDAREPQFGTFTRTNIRNALAWRVPWKDAPLHPSGLLGPVTVKTVKLPTQTP"
BASE COUNT          627 a          854 c          830 g          616 t
ORIGIN      
        1 gttgttcgca gggatacgac acaagccgga agtgctcatt ctccaagatg gggattggcc
       61 gaggcacgac tgaaaatcgg cccacgggcg tcgggggaga gatcgcctta acgacaacga
      121 tccgcgattc ggcggtccac tgcccgtcgg aaccttcggc gatggctatc ccctgacttt
      181 ggacgatgac ccgcactggc aattgcatgg tctcagcatc taccgaaaag gtgtgccgca
      241 ccacagtccg ccgcgttggg gcaagggatt taaccacctg acggcttgcg ccccgcgggg
      301 tttcaaaggt aacgagcacg ccactgagag tgatggcccc aatattctcc agctcgcact
      361 ccaggaccac cgtttgccca acggtcacca cgggcatggt ggggccgaag ctcctcacgc
      421 ggagagcggg cgttagctct aaccgacggg tgtcctcaag ttccccggcc accaccttgg
      481 cgtgaaccgt cagcggttcg tttcgcacca cggccagcgg acaatagagt ggcttgattt
      541 catccggccg caatatgggc agttgcattt ccacaggccg aaacgtggtc ttgcccggaa
      601 gagtcacggt aactttcaca tccttggcgg gctcatcacc gagattcgtc accaagacgc
      661 ggatggtgcc acgttgcccc ggggcctcgg aattctcttc cagcatcacc tttttgatat
      721 ggggaacagg cccgacttcc gccacatact ggatgtcatc aaggaggatt tccccggacg
      781 taccgacaat ccgagctgca aagtggacgc tttttacctt aggattgtcg gaatagtcgt
      841 atttcaagcg agcatggtgc cactggccat cgccgatgtg atcctccggc acggtaaacg
      901 tggctctcgg tgaaccggtg cgttctatcc cctggtcgtt cactgggatg acgtaaacat
      961 tcagcttggc accctttgcc gagacggcct tataccagaa gtccacgccg cctttgagct
     1021 cggagattaa cttcgtccga ttgaggccgg tctctggggt tggtgtgtca gcggatcgta
     1081 ctaagcgtaa tgaccgctgg cctgtgtgag catcttcgga aacactgacc tcgcgtccca
     1141 ccggtcccca atcgaccgga aatccgtttg ggccaagcac ctcgaacgaa ccattcgtgc
     1201 agggattgtc gatcccggtg ggaggacttt ccgcgaagat aggtctttcc aaaaagggca
     1261 aatttccccc gtgacggatc aggctaaaga ggaccacact caataacgtc cgcccaaacg
     1321 acggcatcac ccggcacacg ggaaaatcgc tttttcgaaa gatcatcgtt ggtctccaga
     1381 gttaagggag tttggaatcc gccgattttt cctacggcgt ttgtgtggga agtttcaccg
     1441 tctttaccgt gacaggcccg agtaacccgg agggatgtaa tggggcgtcc ttccagggga
     1501 cacgccaagc caatgcgttc ctgatgttgg ttcgtgtgaa tgtgccgaat tgaggttcgc
     1561 gggcatcgcc cgtcagacgg ttagaccaca cattggccac ccgcaccact aattcgtttt
     1621 ccccctcttt gatgaccccg gtgacatcca gttcataagg gggtgtccac aggatgccga
     1681 gaactttgcc gttaagcgcg atctctccaa caaagcggat ctcgcccaaa ttgaggacca
     1741 cccgccttgg ctccgaaaca ggctcgtaac gaaatgtagt ccggtaggta gccactcccg
     1801 aaaagtactt cacgccggga ttagggtgat ccgtccagga gataaggcgt tctaaggtta
     1861 tgcgttccgg ggcaccccag ccgggaggaa attccacctg ccacggtccc ggaacttgga
     1921 gaacctgggg ggcttccacc gagatggcga gctcccttcc cgattggaaa cgcagtttgt
     1981 aggtccccga ttcagcggca gagagaagag gcaggccctg gccattgggt tggacactca
     2041 caggggcttc gccgctagcc ggggcaaata ccaactcccg cccatcgcgc gtgacactta
     2101 ccacgggatc gagccccggc ccagcggact gcgctgccgt tgtgtcactg aggacgacaa
     2161 aaagacttcc ccgcggaggt aaatcaaggg gcatctcgag cccctgttgg gttttgcgat
     2221 atgccaaggc cggtgtcatt tgcccgctca cagggttcca gatttccggt aacttttcct
     2281 tagcacggag tcgcacaagg aggtgttccc agcgcggtaa acgattccag aagaaataaa
     2341 tctctgctga ggccgtcctg cgatgaacga aatccaccga ggcgtccgcg gcggcatcgc
     2401 aaacaactgc tggtggaatt tccaaccgtt ggagcacctc tcgcagcggt tgtccccaaa
     2461 agaccttccc tttgccgtaa ctgcgttcac ccttgggagg tgggttctca ccccagatgc
     2521 gatcgccgat ggccttaacc ttttcatcgc atgaaggaaa gccggttagg gaggggctat
     2581 gctgaggttt tgttcctaca acaatggctc cctctcggat gagcgattcc aacttctcgg
     2641 ccacctcggg ggtcagttcg ctacccggcg gaagcacaag tagggccacg ccttcttggt
     2701 caggggcgaa cacgattcgg ccgtttttca ccgccgcccg cgtgagaagg gcctcactgc
     2761 tggttaccgt gtagtcgtac ccggggccta attcaggatc gatcgatttc tccataacga
     2821 agttgaaccc acgatcgccg taatagtagc aaacatctgt gacaggctcg ccctgctgaa
     2881 ggaggaagct acaacgggcg atgtaatcga cgaaggcgtc ggcaagt
//
LOCUS       DAOPTR010000463         2923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_27187_length_2923_cov_4.109833, whole genome shotgun sequence.
ACCESSION   DAOPTR010000463 DAOPTR010000000
VERSION     DAOPTR010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27187_length_2923_cov_4.109833"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1671)
                     /locus_tag="PKI05_09350"
     CDS             complement(<1..1671)
                     /locus_tag="PKI05_09350"
                     /inference="COORDINATES: protein motif:HMM:NF027814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOA52453.1"
                     /translation="MMLRTRFLWIIFGLLCWLATPCVAWDDVKQIPLSTLDLSHMSQG
                     WGRPVPKGNVVKQALRIAGKDYQEGVGTHAVSVLHLALDGQVLRFTAEVGIDESAGRR
                     GSVRFQIYADRRLVFDSDVMHGSDPAKPVDVNLEGVKELLLFVDGTEDGIDFDHADWA
                     NAVFYYRGQPPHTVAAPEEPREILTPKPGPAPQLNHAGRYGCRPGRPFIYRIPCTGER
                     PIKFSAMNLPSTLHLDPATGIITGHAPRQRGEYEVQLIAENQHGKDVGKWTIVVGDTL
                     ALTPPMGWNSWYIHYHRVSDADMRAAADAMIDSGMADFGYCYVNIDDCWMVKPGSDDP
                     KLGGPPRDEAGAVRPNGKFPNMKALTDYIHSKGLKAGIYISPGPLTCAGYVGSYQHEE
                     IDAKKFAEWGFDFLKYDWCSYGRVVQAKTREDFMKPYRLMGDILKRLDRDIVYNLCQY
                     GMDSVWEWGAEVGGNCWRTTGDLGLAGGSLNRGIYQVGLKNAKLWEYARPGHWNDPDY
                     LLIGWVGDARVTGEGRPTPLTPNEQYSHMSMWCLMAAPLIFSGDMTRLD"
     gene            1927..2550
                     /locus_tag="PKI05_09355"
     CDS             1927..2550
                     /locus_tag="PKI05_09355"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOA52454.1"
                     /translation="MKNDIEFIYFDLGRVIINFDNDRMCQQMAQVSGVPCERVREILF
                     ATGLQRQFELGEITPDEFYDKYCHLLGRSVDRNALTLAANDIFWLNLPMLPVVAQLQA
                     AGWPLGILSNTCSPHFEWCRSHYAILQKGFRCYALSCDLHALKPDHTIYEKAARLAGV
                     APEKIFFTDDIPAHTEAARAFGYDAVPFQSPRQLVKELRDRGIRFNY"
     gene            2722..2793
                     /locus_tag="PKI05_09360"
     tRNA            2722..2793
                     /locus_tag="PKI05_09360"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2754..2756,aa:Gly,seq:ccc)
BASE COUNT          628 a          835 c          777 g          683 t
ORIGIN      
        1 atcgagcctc gtcatgtcgc ccgagaaaat caagggagct gccatgaggc accacattga
       61 catgtgcgag tattgttcgt tgggcgtcag cggtgtgggg cgtccctcac cggtgaccct
      121 ggcgtcacca acccatccga tgagcaaata atcggggtcg ttccagtgtc cggggcgtgc
      181 atactcccaa agcttggcgt tcttgagccc cacctggtag atgccacgat tgagcgagcc
      241 gcccgccagg cccaaatcgc ccgtggtccg ccagcagtta ccaccgacct ccgcgcccca
      301 ctcccacaca gagtccattc catactggca caagttgtac acaatatctc ggtccagcct
      361 tttgaggata tcgcccatca accggtacgg tttcataaag tcctctcggg tcttggcctg
      421 gacaacacgt ccataggaac accaatcata cttgaggaaa tcgaagcccc attcggcgaa
      481 cttcttggcg tcaatttctt cgtgttgata acttccgaca tatcctgcac aagtgagcgg
      541 tcccggcgaa atgtagatgc ctgctttcaa ccccttggag tgaatgtaat ccgttagggc
      601 cttcatattg ggaaactttc cgttgggtcg gactgcccca gcctcatccc ggggaggccc
      661 acccagtttg ggatcatccg aacccggctt aaccatccag cagtcgtcga tgttgacgta
      721 gcaataaccg aagtcggcca tgcccgagtc aatcatggca tccgccgctg cccgcatgtc
      781 ggcatcactc acccgatgat aatgaatgta ccagctattc catcccatag gcggggtgag
      841 agctaaggtg tcgccgacca caatcgtcca cttccccacg tccttgccgt gttggttttc
      901 cgcaatgagt tgcacttcat actcaccgcg ctgccgaggt gcatgcccag tgataatgcc
      961 ggttgctgga tccaagtgca gtgtggacgg caaattcatc gcggaaaatt tgatcggccg
     1021 ctctcccgta cagggaatcc gataaatgaa cggacgaccg ggacggcatc cgtatctccc
     1081 cgcgtggttg agctggggcg ctgggcccgg cttgggcgtc aatatctcgc gtggctcctc
     1141 gggcgccgca actgtgtgag gaggttggcc ccgatagtaa aacacagcat tagcccaatc
     1201 cgcatgatca aaatcaatgc cgtcctccgt tccatccaca aaaaggagaa gctctttgac
     1261 accttccaga ttgacatcga ccggcttcgc gggatcgctt ccatgcatca catcactgtc
     1321 gaaaaccagt cgcctgtcag cgtaaatttg aaatctaaca cttcccctgc gtcctgccga
     1381 ctcgtcgatc ccaacttccg cagtgaatcg caagacttgg ccatcgagcg ccagatggag
     1441 taccgacacg gcatgagtgc cgacgccctc ctggtaatct ttgcccgcga tgcgaagggc
     1501 ttgcttcacg acatttccct ttggcaccgg ccgtccccat ccttgggaca tatgtgaaag
     1561 gtcaagtgtc gaaagcggga tttgcttaac gtcatcccat gccacacacg gggtggcaag
     1621 ccaacataat aaaccaaaaa tgatccagag aaatcgcgtt cgcagcatca tcgttcctcc
     1681 aaatacggag gttgcttcga caacggttta ttcgccgcta ttgacaacga agcgcgtttt
     1741 tgcaaggttc acggaatcgc cgaactaact acatcggttg tccgtaatcg ttgtaatcgc
     1801 cagggctaga atccgcaagg gtggcgtcct tggcaagacc acagtagagg agactgtggg
     1861 cttcgcgcaa ttcgcaaaag ggtggtctcg ttacaactca actgttatca gtgacagcgc
     1921 gacgtgatga aaaacgacat cgagttcatc tatttcgatt tgggccgtgt cattatcaat
     1981 tttgacaatg accggatgtg ccagcagatg gcgcaggttt ctggtgtgcc atgcgagcgc
     2041 gttcgggaaa tcctgttcgc cacgggattg cagcggcaat ttgaacttgg cgaaatcaca
     2101 cctgatgagt tctatgacaa atattgtcac ttgctgggac gaagtgtcga tcgaaatgcg
     2161 ttgactttgg ctgccaatga tattttctgg ctcaatcttc cgatgttgcc tgttgtggcc
     2221 cagctccagg ccgcaggctg gccactgggg attctctcca acacctgttc gccccacttt
     2281 gaatggtgcc gcagtcacta cgccatcctt caaaaagggt ttcgctgtta cgcgctgagt
     2341 tgtgatcttc acgcccttaa gccggatcac acaatttatg aaaaagccgc ccgtctggcg
     2401 ggtgttgcac ctgaaaagat cttttttacg gacgacatcc ccgcccatac ggaagcggca
     2461 cgtgcctttg ggtatgatgc ggtgccgttt caatcgcctc gccagttggt caaagagctc
     2521 cgcgatcggg ggattcgttt taactactga tcggaccgag ctgataccga tgtcaggtta
     2581 tcttgccggg agccctccac cgggccgacc cgtgggtggt catccccggc ctctggtgaa
     2641 aggtcccgaa cctgggcatt gacacgcagg cgaccaaacc gtacgctggt aatagttagc
     2701 ctggcaaaag ccaggaggat tgcgggagta gctcaggggt agagcgctag cttcccaagc
     2761 tgggggtcgc gggttcgaat cccgtctccc gctttccatg tttatgtggg ttttctcttc
     2821 tgctgtggca tagttacgtt caccagtgcc ggctaggagg gggtaggctt ccacctgcca
     2881 ttagggtgcg ggtgctcttc tgtgcggtct cgtcgtggtg ttc
//
LOCUS       DAOPTR010000464         2917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_27267_length_2917_cov_3.831586, whole genome shotgun sequence.
ACCESSION   DAOPTR010000464 DAOPTR010000000
VERSION     DAOPTR010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27267_length_2917_cov_3.831586"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PKI05_09365"
     CDS             complement(<1..329)
                     /locus_tag="PKI05_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52455.1"
                     /translation="MMHRRQFLQAATLIGLGTLPTWNRRTAQALETSPVKIQQPRHGA
                     VLNRRHGRMLDEGLEILVTGHAPPGRVTVNGVTAERNGEQFSAKVILDQRDIDIRAIH
                     EEGQHED"
     gene            739..1377
                     /locus_tag="PKI05_09370"
     CDS             739..1377
                     /locus_tag="PKI05_09370"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOA52456.1"
                     /translation="MAKLRFTRLLILVASCAVMMCSLLSIPGSDGIAEEERPSLPEHR
                     LTPQPFPIAVIDVATVLENLPQFRSEQMVLRADVERARSHFQERQNTLVQQQQELQKV
                     SPDTPDYKEKIARLQQEISQLQTEFQLQQQEFFNRERQLLLGAYKQIEGAVDQIARER
                     GFVLVLRTSREPSSNNPQELLSYLGREVVWADATIDITKDVVAKIRSTPSKD"
     gene            complement(1426..2337)
                     /locus_tag="PKI05_09375"
     CDS             complement(1426..2337)
                     /locus_tag="PKI05_09375"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HOA52457.1"
                     /translation="MTTNRILPTILAGVILWYVAGSAVVLGEDGAQSEAPKDPAVAKQ
                     SKQQIDEMELRGGKNFIPTKVYSAEEDAAILEMFQGLRVADVCDAMDQVGLRNVGVMS
                     PEIRPAWKDPVNLSHRFVGVAITVRYVPHNMVVPSQLSPEEFNQWSGKWYANLSPEPF
                     VELIRPGTVVVIDDANGADVGSIGSNNIMGWKKRGCVAVVTDATARDLDEIALERIPL
                     YFRGPGRGIRPGRNLIESVNRPVEVGGVLVRPGDIVLGDADGVIVVPREYAEQVAAYA
                     RATLEKDKAARRNLYRALGIPDDVSVQ"
     gene            complement(2438..>2917)
                     /locus_tag="PKI05_09380"
     CDS             complement(2438..>2917)
                     /locus_tag="PKI05_09380"
                     /inference="COORDINATES: protein motif:HMM:NF036831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52458.1"
                     /translation="RATRPKEPPSPDFVKNWLAWGAGPRAAQNIVLTAKARAILHGRF
                     AVTADDIRAMVFPVLRHRIFTNFNADAEGVDVDQILEKILEVVPEPTYGEKLPVSSRS
                     PRKAVAKSPSPPEPPPASAPVTETPLVEPPPAAPIATPPVARPVPIVPPAGEKGGTK"
BASE COUNT          630 a          855 c          793 g          639 t
ORIGIN      
        1 ctgtcctcgt gctgaccttc ctcgtgaatg gcgcggatat cgatatcccg ttggtcgaga
       61 ataaccttcg ccgagaattg ttctccgttt cgttccgcag tgaccccgtt gaccgtcaca
      121 cgaccaggtg gtgcatgacc ggtgaccaag atttctagcc cttcgtccag catccgtcca
      181 tgacggcggt tgaggaccgc cccgtgccgc ggctgttgaa tcttcacagg actggtttcc
      241 agggcttggg cggtcctgcg gttccatgtc ggcagtgttc ctagaccaat aagagttgcc
      301 gcttgcaaga actgacgccg atgcatcatg atgctcctta actctggagg ccatgccacc
      361 gcgccgtgcc ctagtccggc tgcgcgaacc gctctcgttt gtccgacagt ttagcttctt
      421 gttatcggga gtcgcaatca cccggctagg gatttgcgaa gggaatgtct cccgaagcga
      481 gcaggcgccg attctcttgg atacctgtaa ggtcacaacc atttccagat atgttcacct
      541 aacgcgttag ggcaatttca cgttttaaac cggtgccgtt ggccaacatg ggtgacggaa
      601 ctcattacaa tgctagtatt gcggcgaagc gtcagtccgt gcccccagta agtggcgacg
      661 aaaaagcgga cggcaccggc tgattattcc aaggacagct tctaatagca cagcacactt
      721 ctgatgagga gataacccat ggctaagctg cgttttactc ggctactgat actggttgcg
      781 tcgtgcgcgg ttatgatgtg ttcgctctta tcaataccgg gttccgatgg catagctgag
      841 gaggagagac cgagcctgcc cgaacatcgc ttaacgcccc agcctttccc gatcgccgtg
      901 atcgacgtgg caacggtcct ggagaatctg ccacagttcc gcagcgagca aatggtgctt
      961 cgcgcggacg tagaacgggc caggtcccat tttcaggagc ggcagaatac ccttgtgcag
     1021 cagcaacagg agctccaaaa ggtctcgcct gatacgcccg actacaaaga aaaaatagct
     1081 cggcttcagc aggagatttc ccaacttcaa acggagtttc agttgcaaca acaggagttt
     1141 ttcaaccggg agcgccaact tttgctgggg gcttacaagc agatcgaagg ggccgtcgac
     1201 cagatcgcac gggagcgcgg gtttgtcctc gtccttcgaa caagtcgaga accctccagt
     1261 aataacccgc aagaattgtt gagttatctg ggaagggagg tcgtctgggc cgacgccacc
     1321 atcgacatca caaaggatgt ggttgccaag attcgcagca cgccatcgaa agactaattt
     1381 gccaaaagtc acgacttttg ctgggatcgc atattcgccg agcccctatt gcactgaaac
     1441 gtcgtctggg atgcccaacg cgcgatagag gttccttcgt gccgctttat ccttttccag
     1501 tgtcgctcgg gcataggcag cgacttgttc ggcatattcc cgcggaacca caatgacacc
     1561 atcggcatcg ccgagaacaa tatccccggg ccggacgagc acgcctccca cttccacagg
     1621 tcggttgacc gattcaatga ggtttcgccc gggtcgaatc ccacggcctg gccctcgaaa
     1681 atacaagggg atgcgttcga gtgcaatttc gtccaaatca cgggccgtag catctgtcac
     1741 cacggctaca cagccgcgct tcttccagcc catgatgtta ttcgacccaa tcgaacccac
     1801 atccgctcca ttggcatcgt ctataaccac cacagttccg ggacgaatga gctcgacaaa
     1861 tggctcaggc gataggttcg cgtaccactt gccagaccat tggttgaact cttccgggga
     1921 aagctgcgat ggcactacca tattgtgggg cacataacgg actgttatag ccacgccgac
     1981 aaaacgatga ctcaaattga ccgggtcttt ccaggccggc cgaatctcgg gactcatgac
     2041 gcccacgttt cgcagcccca cctgatccat ggcatcacag acatcggcga cacgaaggcc
     2101 ctgaaacatc tcgagaatgg cggcatcttc ctcggccgaa tacaccttgg tggggataaa
     2161 attctttccg ccacgaagct ccatctcgtc aatctgttgt ttcgattgtt tcgcgaccgc
     2221 tgggtccttc ggggcctcgc tctgagcccc atcctctccc aagacgacgg cggatcccgc
     2281 cacgtaccag aggatgacac cggcgaggat agtcggcagt attctgttcg tagtcatgct
     2341 tgttacctcc caaagacggg gcccattttg gcggaccatt cgagccccca aacggacggc
     2401 tacaacggta cggtatgcca aagtcattct gttgcactca cttagtgcca cctttctctc
     2461 ctgccggggg aacgatcggg accggcctgg ctaccggcgg tgtggcgatg ggcgccgccg
     2521 gtggtggttc gactaatggc gtttctgtaa caggggcact tgctggcgga ggctctggcg
     2581 gtgacgggga cttcgccacc gccttccgtg gacttcgaga cgataccggc agtttctcgc
     2641 cgtaggtggg ctcgggaaca acctccagga tcttctccaa aatctgatcc acatctacgc
     2701 cttcggcatc cgcattgaag ttggtaaaaa tgcggtggcg caacacggga aagaccatgg
     2761 cccgaatatc atcggcggtg acggcaaacc gtccgtgcaa aatagcacgg gccttagccg
     2821 tgaggacaat gttctgtgcg gctctcggcc cggcccccca agccaaccaa ttcttgacaa
     2881 aatcggggct tggcggttct tttggccggg tggcacg
//
LOCUS       DAOPTR010000465         2916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_27284_length_2916_cov_3.538972, whole genome shotgun sequence.
ACCESSION   DAOPTR010000465 DAOPTR010000000
VERSION     DAOPTR010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27284_length_2916_cov_3.538972"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PKI05_09385"
     CDS             <1..391
                     /locus_tag="PKI05_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52459.1"
                     /translation="PGEDDSVIRLPCTGNETVLDAIAQVGGISQVSSKRIWIARPSPA
                     GTGCEQILPVDWNAITRGGNTATNYQVMPGDRIFIAHDDMIAFHAWIDKTVAPVERLF
                     GFTGLGTSTVRSLQTMGRGYNRTRSGW"
     gene            504..1208
                     /locus_tag="PKI05_09390"
     CDS             504..1208
                     /locus_tag="PKI05_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52460.1"
                     /translation="MKIAVILIVSAVVLAASAWAQIPEPPVGVGTYVPNTPQQPTGVL
                     ESSTTLPGEVSGLPGTPIPDVAPLPPRTSANRTGTSSKTARSSGIPLPPTAAASVGIA
                     RGTGGWVSGQAALSGGPFPAYMTGYGAGQTAAGFGGGTSVPLEKPFGDYSPAPSVSPY
                     INLYRPEGAFGAAGNYYSLVRPMVQQQQLNRQLSRRLNQMQSALSTGPRSVGRTGAAG
                     GYFMNYYGFYPGLGSR"
     gene            1287..1610
                     /locus_tag="PKI05_09395"
     CDS             1287..1610
                     /locus_tag="PKI05_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52461.1"
                     /translation="MTSKDIPYQPAPPLWTQLPDCESPAEPLPQKGADLVSNDVPNSR
                     SRVVSVLELWLEWNEAYQTLTQRMYEEKTNFHQLEMMADQLDALRLKAVEETRRLLVA
                     LQPCQ"
     gene            complement(2216..2288)
                     /locus_tag="PKI05_09400"
     tRNA            complement(2216..2288)
                     /locus_tag="PKI05_09400"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2253..2255),aa:Ala,seq:cgc)
BASE COUNT          646 a          857 c          787 g          626 t
ORIGIN      
        1 gccgggagag gacgacagcg tgatccgcct gccttgcacc gggaacgaga cggtgttaga
       61 cgccattgcc caagtgggtg ggatttccca ggtctccagc aaacggattt ggattgcccg
      121 gccttccccg gccgggacgg gctgcgaaca gattctcccc gtggattgga acgccatcac
      181 gcggggcggc aatacagcga ccaattacca ggtgatgcct ggcgaccgta tcttcattgc
      241 ccatgatgac atgattgcct tccacgcctg gatcgacaag accgtggccc cggtggagcg
      301 gctatttggg ttcacggggt tgggaacctc caccgtccgt tccctccaaa ccatgggccg
      361 tgggtataac cgcacccgga gcggctggtg atggtcgtca actcgcggat gtggccgtac
      421 aattactttg gccacctcaa gccctcgacc attgcccgat ggagatggaa accaattcgc
      481 ggatttataa acgggagtcg gttatgaaga ttgccgttat cctgattgtc agtgccgtcg
      541 tacttgcggc gtcagcctgg gctcagattc cagaaccgcc tgttggtgta ggtacgtacg
      601 ttccaaatac cccacaacaa ccaaccgggg tacttgaaag tagcacgacg ctgcccggtg
      661 aagtcagcgg attaccggga acaccaatac ccgacgttgc gcctttgccg ccgcgaactt
      721 cggcaaatcg gacgggtact tcctccaaga ccgcgcgttc ctcaggaata cctttgcctc
      781 cgactgccgc cgcttcagtc gggatagcgc ggggcacggg cggttgggtc tcgggccagg
      841 cggccctcag tggtggtccg tttccagctt acatgacggg ctacggggca ggtcaaaccg
      901 ctgccggttt tggcggcggc acatccgtcc ctctggagaa accatttggc gattattctc
      961 cagcaccgtc cgttagcccc tacatcaatc tgtatcgacc cgagggggct tttggggctg
     1021 ctggtaacta ttactctcta gtgcgtccca tggtgcaaca acagcagctc aatcgtcagc
     1081 tttctcggcg gctcaatcaa atgcagtccg ccctgtcaac tggcccccgc tccgttgggc
     1141 gcacgggcgc tgccggcggt tacttcatga actattatgg tttctaccct ggcctgggct
     1201 cgcggtaatt ggttttgtag gaccgcgggg ttatcccggc ttcctttgag tccaaagaat
     1261 cacggatgat tgaggggaaa cacggcatga cgtccaagga cataccctac caaccggctc
     1321 cgcctctctg gactcagctt ccggattgcg agtcgccggc tgaaccgctg cctcaaaagg
     1381 gggcagatct ggtatcaaac gatgtgccca atagcagaag ccgcgtggta tcagtcctcg
     1441 agttgtggct tgagtggaac gaagcctacc aaacattgac gcagcggatg tacgaggaaa
     1501 agaccaattt tcaccaactc gagatgatgg ccgaccaact tgacgcctta cgtctgaagg
     1561 ctgtggagga aacgcgcaga ctgctggttg cgttgcaacc ttgccagtag cgttggagct
     1621 ctaatccttg aatcggatca ccgtgctcgg tctgaagaca tccaaccaaa actagccgta
     1681 ccgggacatt ccgcagttcc cagtgatgag cccttacctt tgaggacctt cggcagatta
     1741 tccctccggt ggagggtacg tttacccaaa cgattggcag aaacgaaccg aacgcccgaa
     1801 ggcctgggcc aatgcaaact ttcgaacgac tttcaacacg atcatcaagc gggctgtgaa
     1861 agcttgtcgc aagctcttat acaaccttcg cgagcgtctt ggtagcgaat ctccccctaa
     1921 cgatggcgac gaagtggttt tcccacgggc ctagtaccgc gccttgtcgg taaattgatc
     1981 tccgaggaaa gacttttgat gtgaccccca ttttggacac aactgggtta gccgcgcgag
     2041 gcatcccggg gagcgaccca ccccagatca aattcgaagc cggcctggcg tcaaaaccga
     2101 catcgccaaa tgcagcgaaa ctgacgacat tcacctgcct ggccgcaagc gacgtcaaga
     2161 tgcaccggtg aacttttatc tgagattagt tatagtttga tgcaaatgcc aaaattggag
     2221 cggaggggac ttgaaccccc gacctctaga gtgcgattct agcgctctcc caactgagct
     2281 accgccccag agtatcttct ggtagttatt ccccgcgact ccccacattt atgaggatag
     2341 cactttgctc gcttccttgc cttgcatacc gtattatcga gcagcccgcg aaagaaccag
     2401 attccagctt tgggcccgcg gacacccaac ctcccaacga aaaatgggca cgcgggactc
     2461 gaacggattt ccgcaaatgg cgatttaacc gcaatttacg acgataccaa aaaatccttg
     2521 gccatgccag cggccatact gccgaccaaa acaatcccag acccgtccga cttagcttcc
     2581 ttggtggata tcatccgtgc gctacctgtc gaggcccggg aacaactacg ccggcttttc
     2641 gaccaattat aatggtccat gcgggcttgt ctagggcggt gaagggcgag aaggctagga
     2701 caacgaattt gtttcacctc aaatgggccg acatcatgtt ccctcttcgc gcccatgggg
     2761 gctagtccat gtggattagc ttcctgacaa agcttctacc gcccacaaac accacgaagc
     2821 caccaaccct gaatcttgga cgggcatttg tggcgccctt gggacgggtc tgttacccca
     2881 catcagtctg ggtcaagagt tgtccaacct aaagga
//
LOCUS       DAOPTR010000466         2913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_27328_length_2913_cov_3.637159, whole genome shotgun sequence.
ACCESSION   DAOPTR010000466 DAOPTR010000000
VERSION     DAOPTR010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27328_length_2913_cov_3.637159"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PKI05_09405"
     CDS             <1..189
                     /locus_tag="PKI05_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HOA52462.1"
                     /translation="QAGTPGRDGLGLLRQNALEASNVEPVRELIDLITTQRAFEMNSQ
                     AVQVGDQMMQLVSNLRRY"
     gene            209..1264
                     /gene="flgA"
                     /locus_tag="PKI05_09410"
     CDS             209..1264
                     /gene="flgA"
                     /locus_tag="PKI05_09410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HOA52463.1"
                     /translation="MKRWCIPAVILGAFLLWAGASASAATFRLRSDFTATRTPITLRD
                     LVAIEDCDPETRDALGSLPLFPAPPIGEARFVNRDEIRDLLILRGIDMRMHTWTGAFQ
                     IRIVTSPTAPTPPEREKNSSQLQTRAQERLTAALEDYWRRTFPESPKCRFEFVLSPED
                     VKWFSAADASLVVNRVQALSADTWLVDVLTQVGQEKNVTQIKVKCGPPSQVVVAARPL
                     SRDVIIGRSDVALSERAGPVDSEIATQIEEVVGKQLTLAISAGKPIPRSALRDPVVIR
                     RGDLIQVTVRASGVTVRTAARAKDDGVVGHLIPIETLDGKGKTIMARVVGPKQVEIYA
                     MSPEAEASKNLLSRGGL"
     gene            1303..1971
                     /locus_tag="PKI05_09415"
     CDS             1303..1971
                     /locus_tag="PKI05_09415"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HOA52464.1"
                     /translation="MSVAQHGLLAITVLLCAVFHQVALGQTSSLLGGSQGTPPTLDRL
                     SWTYQPPPQQKVLRLHDILTVLVDEKTQVIAEGQMDRRKKADGKFVLSDWVLLKKWSL
                     IPDPQSLGDPTIGGQMENKYRAESELESREAMKLRVACEIVDIRPNGNLVIEGRRTIQ
                     VNNDIWEIYLTGTVWPQSILPDNTVQSENVADLRVIKREVGHVRDGYRRGWLLKWLDE
                     HQPF"
     gene            2017..>2913
                     /locus_tag="PKI05_09420"
     CDS             2017..>2913
                     /locus_tag="PKI05_09420"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOA52465.1"
                     /translation="MTMRHRFIGAIVCLCVFMTGGALWAAVPLKTICRVKGQEDNNLH
                     GLGLVVGLKGTGDGSSFLPTIRSMAVALEMLGNPLARDAQTELKDAKNVALVLVTATV
                     PPAGARQGDRIDCQVHSVGSCKSLAGGRLFLTPLVGPDPRDRRVYALAEGSITIESTD
                     TPTVGRITGGCRLEADFFNPFVKDGRVTLVIDPPYADFQVAQDIVELINSQMTVQSGG
                     MMLARAINQVNVEVLIPRQYADDPVLFVSQLMELPLMDVAVGPRVVVNERTGSIIISG
                     DVEIGPVVVTHGNMVIQAGGAQF"
BASE COUNT          646 a          785 c          847 g          635 t
ORIGIN      
        1 caggccggaa caccggggcg cgatgggctg gggctgctcc gccagaatgc cctggaggcc
       61 tctaacgtgg agccggtgag ggagttgatc gaccttatca ccacgcaacg ggctttcgag
      121 atgaactcac aagccgttca ggttggcgat cagatgatgc aattggtttc caacctgcgg
      181 cggtactaag tgttctgagg atcatgccat gaaacggtgg tgcataccag cggtgatcct
      241 tggggccttt ttgctgtggg ccggagcgtc tgcgtcggcg gccacgtttc gcctccgctc
      301 ggatttcacc gcgacccgaa ctcccatcac cctgagagac ctggtggcaa ttgaggattg
      361 cgaccctgaa acacgtgacg ctctgggttc tttaccgctg tttcctgccc caccgatcgg
      421 agaagcgcgt tttgttaacc gcgatgaaat tcgtgatcta ttgattctgc gcggtattga
      481 catgcggatg cacacctgga cgggtgcctt ccaaatcagg attgtcacaa gccctacagc
      541 gccgacaccg cctgagaggg aaaaaaatag ctctcaactt cagactcgtg cccaagaacg
      601 attgacggca gccctagaag actactggcg ccgcacgttt cccgagtcgc caaagtgccg
      661 gtttgaattc gtgttgtcgc ccgaggacgt aaagtggttt tccgccgccg atgcgtccct
      721 cgtggtcaac cgcgttcaag ccttgtcggc cgatacgtgg ctggtcgatg tgctaacgca
      781 ggttggtcag gaaaagaacg taacccaaat caaagtcaag tgcggtcctc cgtcgcaggt
      841 tgtggttgcc gcaaggccac tttcccgcga tgtcatcatt gggcggtccg acgtggcctt
      901 gagtgagcga gcaggacctg ttgactccga aatcgcgaca caaatcgaag aggtcgtggg
      961 caaacaactg accttggcaa tctcggcggg aaaaccgatt ccccgctcgg ccctccgaga
     1021 tccagtcgtt atccgtcggg gtgacctgat tcaggttact gtgcgggcct ccggcgtgac
     1081 ggttcgcacg gcagcacggg cgaaagacga tggggtggtc ggtcatctca ttcccatcga
     1141 aacgttggat gggaagggta agacaatcat ggcccgggtc gttggcccca aacaagtcga
     1201 aatctacgcc atgagcccag aagctgaggc atctaaaaac ctactatcac gtggtggact
     1261 atgacccttg atccgaaata tctaaggcaa aagaagacaa gcgtgtcggt agcgcagcat
     1321 gggctccttg ccataacggt gttactgtgt gctgtattcc atcaggttgc ccttggccaa
     1381 acaagtagcc tgctgggcgg ctcgcaggga acaccgccca cgctggatcg tctgtcctgg
     1441 acatatcagc ccccgccaca acaaaaagtc cttcgcttgc acgacatttt gaccgtcctg
     1501 gtggatgaaa agacccaagt catcgctgag ggtcaaatgg accgacgaaa aaaggccgac
     1561 ggaaagttcg tgttgtcaga ctgggttctg ctcaaaaaat ggtccttgat ccccgatccc
     1621 cagagtttgg gagatcccac tattggcggg caaatggaga acaagtaccg cgccgaatct
     1681 gaacttgaaa gtcgcgaggc catgaagctt cgtgtggctt gtgagattgt tgatatccgt
     1741 ccgaacggca acctcgtgat tgagggccga cggacaatcc aggttaataa cgacatttgg
     1801 gagatttacc taaccgggac ggtttggcca cagtccattt tgcccgacaa cactgtgcaa
     1861 agcgaaaacg ttgctgatct tcgggttatc aagcgagaag ttggccacgt gagggatggt
     1921 tatcggcgcg gctggctcct caaatggctg gacgagcatc agcccttcta ggagaccacc
     1981 aggttatttt gctaacaaag ggagcatgga cgcaccatga caatgcgaca tcgctttatc
     2041 ggcgccatcg tttgcctctg cgtgttcatg acgggcggcg ccctctgggc ggcggtcccg
     2101 ctcaagacaa tttgtcgcgt caaaggccaa gaggataaca accttcacgg tctgggactt
     2161 gtggtgggct taaagggtac tggagacggc agtagctttc tccccaccat ccgcagcatg
     2221 gccgtggcct tggaaatgtt gggaaatccc cttgctcgcg atgcccaaac cgaattgaag
     2281 gatgccaaaa atgtggccct tgtcttagtc acggcaacag tccctccggc gggcgcgcgg
     2341 caaggcgatc gaatcgattg ccaggtccac tcagtgggat cgtgcaaaag tctggcaggg
     2401 ggacgactat tcctgacacc gctcgtcggg cccgaccctc gggatcgccg tgtttatgcc
     2461 ctggctgagg gttccattac cattgaaagc actgacacac caaccgtggg aagaattacc
     2521 ggtggctgcc ggttggaggc agatttcttc aatccgtttg tcaaagatgg ccgcgtgaca
     2581 ctggtcattg atccccccta cgccgacttt caggttgccc aggacatcgt tgagttgatc
     2641 aattcccaaa tgacggtcca aagtggtggg atgatgctgg ctcgggcgat caatcaagtc
     2701 aacgtggagg ttttgattcc ccggcaatat gccgatgatc ctgtcctgtt tgtgtcgcaa
     2761 ctcatggaat tacccctcat ggatgtggcc gtcggtccga gggtcgtggt caatgaacgg
     2821 acaggcagca tcattatcag cggcgatgtg gaaattggcc ccgtggttgt gacccacgga
     2881 aacatggtga tccaggccgg aggtgcccaa ttt
//
LOCUS       DAOPTR010000467         2912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_27341_length_2912_cov_3.505425, whole genome shotgun sequence.
ACCESSION   DAOPTR010000467 DAOPTR010000000
VERSION     DAOPTR010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27341_length_2912_cov_3.505425"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="PKI05_09425"
     CDS             <1..753
                     /locus_tag="PKI05_09425"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HOA52466.1"
                     /translation="CYALSDIEAERIKDFCERGGTVIADFCCGLFDQHGKGRKRGALD
                     DLFGVSHDGSETARDFFGDRLWVETDQDAAYGYKRYRELFATISCQLEKGYAVAERRL
                     PVGTVRKVGRGSAVYLNLSPQRYLQYREEGTASDELRQPFLQPIHNAGIRPWIWVTSE
                     GRRPANTEVCYWKKGDRVYALVVQSAPVTGSMRGGGGAEQLKVQTISIEVEFARNVRD
                     VRNERTGESLGDGNRFRFDFPLAEAVFLSFLE"
     gene            complement(993..1415)
                     /locus_tag="PKI05_09430"
     CDS             complement(993..1415)
                     /locus_tag="PKI05_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007340654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOA52467.1"
                     /translation="MKSYTQYLTFNIPARMAFVNITKQVEEAVKASGVREGLCLVNAM
                     HITASVFINDDEPGLHEDYKRWLEKLAPYDPSPQTYHHNRTGEDNGDAHHKRQIMGRE
                     VVVAITDGKLDFGPWEQIFYGEFDGRRPKRVLIKIIGE"
     gene            complement(1544..>2912)
                     /locus_tag="PKI05_09435"
     CDS             complement(1544..>2912)
                     /locus_tag="PKI05_09435"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOA52468.1"
                     /translation="LIYRFIAGLGIGGQFGLGTAIVAETLPDRARPKALGFLQAFSAF
                     GNISAGVVGIIFAQLVLAGYIQGQSWRWMFGVGILPAFLIVFVMFWLKEPERWKQAVA
                     EGKISKQKAGSLKELFGDPRWRVRAIVGIILAAAGVIGLWGIGVFGVDLVQSIFRTNI
                     TAEYRAKGAAKEDQQFVALVFKTPGAVEKVSEAGILPRDMIGQDPNDKDAQYLLAAAL
                     TLASRKERVTLEGALKLLDQPWTDDGSPLAKLDRKRQAQTPEDRARRKEFVEKAPVEQ
                     TSLEDHLTRLKERAKNVGGYVQRWGSIQLLLFNIGAFFGIYAFSMVTDKLGRKKTFAL
                     FFLFAMVVTAFVFLTMRKWHHVLWMAPLMGFAQLSVFGGYAIYFPELFPTRLRSTGTS
                     MCYNIGRYVSAFGPFLQGYLSGVVFAGFAEPMRVAGATMCLFFLLGIVVLPFAPETKD
                     QPLPE"
BASE COUNT          655 a          836 c          815 g          606 t
ORIGIN      
        1 tgctacgcat tgtcggatat tgaggccgag cgaattaaag acttttgcga acggggtgga
       61 acggtcatcg ccgacttctg ttgtggactt tttgaccaac acggcaaggg gcgtaaacgg
      121 ggagccttgg acgatctgtt tggggtttct catgatggca gcgaaaccgc ccgggatttc
      181 tttggtgatc ggctgtgggt ggaaacagat caagatgcgg cctacggcta caaacggtac
      241 cgggaacttt tcgcgacaat ctcgtgccaa cttgagaagg gctacgccgt agccgaaaga
      301 cgtttgccag tcgggactgt acgaaaggtc gggcgcggct cggcggtgta tctcaatctt
      361 tcaccccagc gatatcttca gtatcgtgaa gagggcacgg ccagcgacga gctccgccaa
      421 ccgtttttgc agcctatcca caatgccggc attcggccgt ggatttgggt gaccagcgag
      481 ggccgccgac cggccaacac cgaagtttgc tactggaaaa agggggaccg ggtgtatgcc
      541 cttgttgtgc agagtgcgcc tgtcaccgga agcatgaggg gtggcggcgg ggccgaacag
      601 ctcaaagtgc aaacgatctc tattgaagtg gagttcgctc gcaatgttcg agatgtgaga
      661 aatgagcgaa ccggggaatc gttaggtgac ggcaatcgct tccggttcga cttccccttg
      721 gcagaagcgg tcttcttgag ctttttggaa tagcccgaag aacgaaaggg acggctggaa
      781 aactcccgaa aatgggaggg acggcgtcct cgccgtcccg tgtgcaccgg cgctacagac
      841 aaacggggca accacagggg gttgccccta cggttgccga acccattgaa acctgtaggg
      901 gcaggcccct gtgcctgccc caaaacacaa ccaatccccg agccaattgc tcgccgcgga
      961 cggcaaccct cccgaggcta ttacggcgcc atttactcgc caatgatctt gatgaggacc
     1021 cgcttggggc ggcggccatc gaattcgccg taaaaaattt gctcccaagg gccgaaatcg
     1081 agcttgccgt cggtgatggc gacgactacc tcgcgtccca tgatttgacg tttgtggtgc
     1141 gcgtcgccat tgtcttcgcc cgtccgattg tgatgatacg tttgcggcga cggatcgtaa
     1201 ggcgcgagct tttccagcca gcgtttgtaa tcctcgtgga ggcccggctc atcatcattg
     1261 ataaacacgc tggccgtgat gtgcatggca ttgaccaggc agaggccttc tcgcacacca
     1321 ctggccttga cggcctcctc aacttgcttg gtgatattca caaacgccat gcgggcggga
     1381 atgttgaacg tcaaatactg cgtataactc ttcatggaac atcctccttg ccgccgagcg
     1441 agccatctcc ggttgtcgca tcaatccacg aacacccttg ttcgatctcc acggtccggc
     1501 gcatgatccg gtccttccgc ggaaaccaca cccaagcgtc gccttattct ggcaagggct
     1561 ggtcttttgt ttcgggtgca aaaggcagaa ccacgatacc cagcaggaaa aagagacaca
     1621 tcgtggcccc ggcgacgcgc atcggttcgg caaaaccggc gaatacaacg ccgcttaggt
     1681 atccctgcaa gaatggacca aaggccgaga cataccgccc gatgttgtaa cacatcgagg
     1741 ttcccgtgct ccgcagccgt gtcgggaaca attccgggaa gtaaatggcg tagccgccga
     1801 agacggaaag ctgggcaaag cccatgagcg gggccatcca cagaacgtga tgccattttc
     1861 gcatcgtcaa gaagacaaag gccgtcacga ccatcgcaaa aaggaagaac aaggcgaatg
     1921 tttttttacg ccctagtttg tcagtcacca tgctgaacgc gtagatgcca aagaaggccc
     1981 cgatattgaa gagcagcagt tggatgcttc cccacctctg cacgtaacca ccgacgttct
     2041 tggctcgctc ctttaggcgg gtcaaatggt cctccaaact tgtttgttcg actggcgctt
     2101 tttcgacgaa ttcctttcgc cgcgcacggt cctcgggcgt ttgggcctga cgcttgcggt
     2161 cgagcttcgc caggggtgaa ccatcatcgg tccagggttg gtcgagcagc ttcagggcac
     2221 cttccaacgt gacgcgttct ttgcgggatg ccaaggtcaa agcggctgcc aacaagtact
     2281 gcgcgtcctt gtcgttcggg tcttgcccaa tcatatcgcg aggaagaatt cctgcctcgg
     2341 agaccttctc caccgcaccc ggtgttttaa agaccaaagc gacaaattgt tggtcctctt
     2401 tggcggcgcc ttttgcgcgg tattccgctg tgatattggt gcggaagatt gattggacaa
     2461 ggtccactcc gaaaacgcca atgccccaca aaccgatgac gccggccgcc gccaagataa
     2521 tgcccacaat ggcgcgtacc cgccagcgag gatccccaaa aagctctttc agcgagcctg
     2581 ccttctgctt agagattttt ccctcggcca cagcttgttt ccaacgttcg ggttccttta
     2641 gccagaacat gacaaaaaca atcaggaatg ccggcaaaat cccgacacca aacatccacc
     2701 gccagctttg gccttgaatg tatccggcca acacaagctg ggcaaagata attcctacca
     2761 cgccggcaga aatgttccca aacgccgaga acgcctgcag aaatcccaag gccttgggtc
     2821 gcgcgcggtc tggtaacgtc tcggcgacga tggccgtgcc taagccgaac tggcccccaa
     2881 tgcccagtcc tgcgataaac cgatagatca gg
//
LOCUS       DAOPTR010000468         2911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_27359_length_2911_cov_2.893207, whole genome shotgun sequence.
ACCESSION   DAOPTR010000468 DAOPTR010000000
VERSION     DAOPTR010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27359_length_2911_cov_2.893207"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PKI05_09440"
     CDS             <1..546
                     /locus_tag="PKI05_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52469.1"
                     /translation="YLDSAQTDQYGGRYGYLPGHAATPTMTAEALLCRQYLGWPREHR
                     GMKAGAYYLLENLPSAKKLDSSQPWFYYIYYATQVMRNMGGTFWQKWNDAMKATLLPT
                     QRKTGSLAGSWDPVERYGPHGGRIYVTSLAICTLEVYYRHMPLYDSVPVIASLPHSGG
                     QADTALGDNPTGKEKREVLTP"
     gene            complement(757..1062)
                     /locus_tag="PKI05_09445"
     CDS             complement(757..1062)
                     /locus_tag="PKI05_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52470.1"
                     /translation="MPSIDQELGATKTGGFWHRQGHAAPQQSESGWEAVPSEHEVPQQ
                     DEVHESEGASTAPGEQISHRPVNAHVQREVELNSQITAASSAQGIPSLFSMKAYPMA"
     gene            1334..1750
                     /locus_tag="PKI05_09450"
     CDS             1334..1750
                     /locus_tag="PKI05_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52471.1"
                     /translation="MNRRLFVTAICAVSLLGLAGWIAFGSGQSEKQSRKTGVCRCPNC
                     TCDHCTPERCCCEGGGPCDCNCECCQTAACCQDNCQAGNGCAKECNCPGCQCAEGGTC
                     TCPDCKCAEGGPCTCEGCPCGAPASQPVACCGKTAA"
     gene            2428..>2911
                     /locus_tag="PKI05_09455"
     CDS             2428..>2911
                     /locus_tag="PKI05_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52472.1"
                     /translation="MGKGTPPAVIVVSETASWLERLAALEVRRYLYLRTGQLLPIQHG
                     LPNGNADVILLARSDRLLESDLLPKETLELYRDSLRERIQRAGKHGYLLWTLPSQGRH
                     VLLLVSSQDVGLLYAAYRLAEHYGVRFYLEGDVIPDMRLAWSLAQIDEVRRPLFEHRG
                     I"
BASE COUNT          608 a          820 c          829 g          654 t
ORIGIN      
        1 tacctggata gtgcgcaaac cgaccaatac gggggccgat atggttatct tccgggtcac
       61 gctgccacgc caacaatgac ggccgaggcc ctgctttgcc gacaatacct gggttggccg
      121 agggaacacc gtggcatgaa ggctggggcc tactacctcc tcgaaaacct accctcggct
      181 aagaaattgg actcctctca gccgtggttt tattacatct actacgccac ccaggtgatg
      241 cgcaacatgg gcgggacgtt ttggcagaaa tggaatgacg ccatgaaggc cacgctcctc
      301 cccacgcaac ggaagacggg atcactggca ggaagttggg atcctgtgga acgttacggt
      361 ccgcatggcg ggaggatcta tgtcacgtcg ttggccattt gcaccctgga agtctattac
      421 cggcacatgc cgctgtacga ttccgtccct gttattgctt ccctccccca cagtggggga
      481 caggccgaca cagctttggg cgacaatccc acgggaaaag agaagcgcga agttctcacg
      541 ccttgatggt ttcccggcca caacagtacg gagctttccg gcgacatggg gcaggtctgg
      601 aggcccgccc ctatgaaggt ttggaatacc cgcacctgac taaaagtggg ttgggtcatt
      661 tgagcgcagc gaaataatgt tgccaccgcc agacctaatt gctataattc agtccagaaa
      721 cgtccgatcc ttgaataagc gaatcgtagc cggttatcag gccatagggt aagccttcat
      781 gctgaacaga ctaggtatcc cctgtgcact actggcagcc gtgatttggc tatttagctc
      841 gacctcacgc tgcacgtgcg cattaaccgg gcgatgcgag atttgctccc ctggcgcggt
      901 ggaagcacct tcggactcgt gcacctcgtc ctgctgtggc acctcgtgct ctgaagggac
      961 cgcctcccaa cccgattcgg actgttgcgg tgccgcgtgc ccttgtctgt gccagaagcc
     1021 gcctgtcttg gttgccccga gctcttggtc aatcgatggc atcgctcagc cctgcctgtg
     1081 ggacagcgaa ccgttgaccg ttcaacccag gacaggcaag gtggtggctt ctttggatac
     1141 gggcgtcaca ttatccttag ctcttctatg tcggtggctg aaataatcac cgggcgtggc
     1201 gtcctctttg gtccttcgat tttcttctga agaggcgtcc tctctgcatt tccgggtttc
     1261 ccaggtgaag tgctggctaa ggttttggtt tttgaccatt tcaatgaacc taaataagaa
     1321 aaggagatga aaaatgaatc gacgtttgtt tgtaacggcc atttgtgcgg tatctttgct
     1381 gggcttggcc ggctggatcg cttttggcag tggccaatcc gaaaagcagt cccgaaaaac
     1441 cggcgtgtgc cgttgtccca actgtacgtg tgaccactgc acgcccgaac gttgctgctg
     1501 tgaagggggt ggcccctgcg actgcaactg cgaatgctgc cagacggctg cgtgctgcca
     1561 agacaactgt caggcaggca acgggtgcgc caaggagtgc aactgccccg gttgtcagtg
     1621 tgccgaaggc ggcacctgca catgccccga ttgcaagtgc gccgagggcg gtccctgcac
     1681 atgcgaggga tgtccctgtg gggcgccggc ctctcaaccg gtggcttgct gcggcaagac
     1741 cgctgcatga ttgaccatca ccgcaagtag ggacgaacac ggatcagtct tgcggtgtaa
     1801 atcgctgaag cgtgcgacga gtaccctaaa ggtgtcgttg cgaacgtcaa caggccttcg
     1861 aacggcgtca gccgttcgaa ggttttttat tgtgcaacgt ttaggcaaag agtctatccc
     1921 aaaaagtcct cctctccctc cgggagaggg ttgggtgagg gcaagcaatc gtgggagtcg
     1981 atcgagaaaa ccggcagcga ttcgagcgac ccccactctc gacccctctc ccgctgaacg
     2041 catgaaggga gataggctct aagggccaag tgcccgaggg gcaaacatac gccatgtgcc
     2101 ttttttgaca ccagagcacg ctggcggttc cgagttggag atgacccgcg ctcatgtacc
     2161 agcgaattgg tcttcgacaa cttcgaaggc caccccagtc catctcttgc ttgggacccc
     2221 taatccctcc aaaatagagc caccaaaaca ttcccaaaaa cgcaacaagg gaggaatatg
     2281 atgtccgcga cgtcggtcgc caacggggtc attgtggcaa ttttcgaaag ccaaaaaccg
     2341 cccaggatat tttccggctg gaggggatta ctcttgatcg gcctttcatg ttgccttttc
     2401 gggcggggtc tcgccgcgga gccgtcaatg gggaaaggca ctccgcccgc agtcattgtc
     2461 gttagcgaaa ccgcaagctg gctggaaaga ctggctgcct tggaggttcg gcgctacctc
     2521 tatcttcgca caggccagtt gctccctatc caacatggcc tgcccaatgg taatgccgat
     2581 gtgattctgc tcgcacggag tgatcgtctg ctggaatccg atctgctccc aaaggagacg
     2641 cttgagctct atcgagatag cctccgggaa cgaattcaac gagccggaaa acatgggtat
     2701 cttctttgga cactgccctc gcagggccga catgtcctgc ttctggtgag ctcccaggat
     2761 gttggccttc tttatgccgc ctatcgcctg gccgagcact acggcgtgcg attttatttg
     2821 gagggtgatg ttattcccga catgcggctt gcatggtcat tggcccaaat tgacgaagtt
     2881 cgtcggccac tttttgagca ccgcgggatt c
//
LOCUS       DAOPTR010000469         2910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_27370_length_2910_cov_3.242732, whole genome shotgun sequence.
ACCESSION   DAOPTR010000469 DAOPTR010000000
VERSION     DAOPTR010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27370_length_2910_cov_3.242732"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="PKI05_09460"
     CDS             complement(<1..1088)
                     /locus_tag="PKI05_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOA52473.1"
                     /translation="MKVRVNPFAKLLGFLWVFSVLALGGVTLAQDGHSNSPGTGPDPQ
                     IYQACVKKGIDFLINRGQLPDGSFSPEAGVGITGIAVFALLQHGLPVDHPAIRSGLGY
                     IERYIQPDGGIYSPDSFYKNYETCIAIMCLQAANKDGRYQDVIARAERFAKDIQWDES
                     EDISPSDYAYGGAGYGRHRRPDLSNTAFLIDALKSLGRGPDDPAIQKALIFVSRCQNL
                     ETEHNTTPFANKNPDGGFYYTWAAGGESMAGKTPTGGLRSYGSMTYAGLKSMIYAGVK
                     ADDPRVQAAKRWIAEHYDLESNPGLGQQGLYYYYHTFAKTMSALGEPYFVDASGKRHD
                     WRAELVAALVKRQREDGSWINMADRWMEG"
     gene            complement(1341..2723)
                     /locus_tag="PKI05_09465"
     CDS             complement(1341..2723)
                     /locus_tag="PKI05_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOA52474.1"
                     /translation="MSKATLLLVDDDRHLLNAMAVWLREQGYKVDTGASVQDARRELT
                     QRGYDVAIIDIRLPDGDGFEVLRFAKERYPEMPVILITGYGTVDLAVEAIRAGAFDFL
                     TKPLIDEELQLSIERALNQRRVIEENKNLKCQLDMRYGLDNIVGRDHRMIRIYDMIEA
                     VADTKATVLITGESGTGKSLIARAIHRRSGRRDKPFVEVACGALPETLLESELFGHVA
                     GSFTGATSDKIGKFKQADGGTIFLDEIGTASPALQVKLLRVLQDYQFEPVGGTQTITV
                     DVRVILATNEDLQKAVQMGRFRQDLYYRINVINIELPPLRERVTDIPLLAEHFLRRIC
                     EEMGRPVKTLSEEALAALQAYRWPGNVRELQNVLERAVLLGRHQTITLEDLPPEVAAA
                     LSPSYANADTAPLKQALQLPEKQIILQMLELHNWNRQETAKALGINRTTLYKKMRRLG
                     IQVPQGSTHT"
BASE COUNT          619 a          800 c          845 g          646 t
ORIGIN      
        1 ccctccatcc agcggtcggc catattgatc caagaaccgt cttcgcgctg gcgtttgacc
       61 aaggccgcga cgagttccgc gcgccagtcg tgtcgcttgc cggaggcatc gacaaagtag
      121 ggttccccta gggcgctcat cgtcttggcg aaggtgtgat agtagtagta gaggccttgc
      181 tggcccaagc cagggttgga ctcgaggtca taatgctcgg cgatccaacg tttggcggct
      241 tgaacgcggg ggtcatcggc cttgacgccg gcatagatca tacttttcag acctgcatag
      301 gtcatcgaac cataactgcg gagaccaccc gtgggggtct ttcccgccat actttcgccc
      361 ccggcagccc atgtgtagta aaacccgccg tcgggatttt tattggcgaa gggggtggtg
      421 ttgtgttcag tctcgagatt ttggcaccgc gaaacgaaga tcaaggcctt ttggatcgcc
      481 ggatcgtctg gtcctcgacc gagtgacttg agggcatcga tcaaaaaggc cgtgttggag
      541 aggtcgggac gccgatgacg accatatccg gcaccgccgt aagcgtaatc actgggagaa
      601 atgtcctcgc tttcgtccca ttggatgtcc ttggcaaagc gctcggcccg ggcgatcaca
      661 tcttggtagc gaccatcctt gttggccgct tgaagacaca taatggcgat acacgtttcg
      721 tagtttttgt agaagctgtc gggggagtag attccaccgt cgggctggat gtaccgttcg
      781 atgtagccca ggccagagcg aatggccggg tgatccaccg gcaatccgtg ctgaaggagg
      841 gcaaacacgg cgatccccgt gatccccact ccggcctccg gggagaacga accgtcgggc
      901 aattgtccgc ggttgataag gaaatcaatg ccttttttga cacaggcctg gtaaatctgc
      961 ggatcggggc cagtgccggg ggaattacta tgtccgtcct gcgccaacgt gacaccgcct
     1021 agtgccaata ccgaaaagac ccaaagaaaa ccaagcaatt tagcaaacgg gttaacccta
     1081 actttcatag cacaaactcc tgggcaaaaa atcctgcgcg agctgactgc cgtcgagtaa
     1141 gcgagacgaa caggtcaatc aaagcaaagt ccgtgccgag acacagtccc ttttccagcg
     1201 gtgggaaaat atgtgggatt gtttgggcgg tttccaacat catagtcgcg ggaaggcggc
     1261 cgcggcaagc gcgacagtcg tttggcgcaa catggtgtgt cgaaaaatgc gacatgatta
     1321 gggcatcgcc ttcagtacca tcaggtgtga gtagaacctt gcggcacctg gataccgagt
     1381 cgcctcattt tcttgtagag cgtcgtgcgg ttgatgccga gggccttggc ggtctcctgt
     1441 cgattccagt tgtgaagctc cagcatttgc aaaatgattt gcttttcggg caactggagt
     1501 gcctgcttaa gcggtgcggt gtcggcgttg gcataacttg gcgaaagagc cgccgctacc
     1561 tcaggcggaa ggtcttccag tgtgatggtc tgatgtcttc ccaataggac ggccctttcc
     1621 aacacgtttt gcagttcgcg gacgttgccc ggccagcggt aggcttggag ggcagccagg
     1681 gcctcctcgc tgagcgtttt cacggggcgt cccatctctt cgcaaatacg gcgaaggaag
     1741 tgttcggcca aaagtgggat gtcggtgacc cgttctcgca acggtggcaa ttcgatattg
     1801 atcacgttga tgcggtaata aaggtcttgg cgaaatcgcc ccatttgaac ggccttttgg
     1861 aggtcctcgt tggtcgccag gatcacccgc acatctacgg taatcgtttg cgtgccaccg
     1921 accggctcga attgatagtc ttgcaagacc cgcaagagct tgacctgcaa ggcgggactg
     1981 gccgtgccga tctcgtcgag gaaaatcgtg ccgccgtcgg cctgcttgaa tttgccgatc
     2041 ttgtccgacg tcgcacctgt aaacgacccg gccacatgtc cgaagagttc actttctaaa
     2101 agcgtttcgg ggagggcacc gcaggccact tcgacgaaag gcttgtctcg ccgaccgctc
     2161 cgccggtgga tggcgcgggc aatcagggac ttaccagtgc cgctttcccc cgtgatcaag
     2221 acagtggcct tagtatccgc cacagcctcg atcatatcgt agatgcggat catgcgatgg
     2281 tcgcgaccca cgatgttatc gagaccatag cgcatgtcta gttggcactt gagatttttg
     2341 ttttcttcga tgacgcggcg ctgatttaat gcccgctcga tggacagttg caattcctcg
     2401 tcgatgagcg gctttgttag gaagtcaaag gcccccgccc gaatggcctc caccgccaga
     2461 tccaccgtgc cgtaccctgt gatgaggatg actggcatct cgggataacg ttccttggca
     2521 aaacggagga cctcaaaccc gtcgccatcc ggcaaacgga tgtcaataat ggccacgtcg
     2581 tagccacgct gggtcagttc gcggcgggcg tcctggacac tggcccccgt gtcaaccttg
     2641 tagccttgtt cgcggagcca gacggccatc gcatttagga gatgccggtc atcgtccact
     2701 aaaagcaacg tggccttgct catcttggtg acctttgttg ctcaaaacca actttccgat
     2761 tggcgaggtg ctgcggaaaa gagttgctcc ctcccttgcc taccgtaaaa accccgttgg
     2821 taacgcgatc ccgcccatgg caagggaggc cgtcgaaaag tatctacgtc agaaacggcg
     2881 gcttgtcaac atgagaaaaa taggaaacat
//
LOCUS       DAOPTR010000470         2891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_27582_length_2891_cov_3.232017, whole genome shotgun sequence.
ACCESSION   DAOPTR010000470 DAOPTR010000000
VERSION     DAOPTR010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27582_length_2891_cov_3.232017"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PKI05_09470"
     CDS             complement(<1..385)
                     /locus_tag="PKI05_09470"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HOA52475.1"
                     /translation="MKLQTKVVGFSVIGVLLTATVTTGLVAINKSRFGEQVRTEIDAL
                     GKDTCRHIALNVREMLEVYHKELIKRLDQGMEAAKEMVARSGDFTIKEEKVTWQAKNQ
                     FTGQVQNVELGKMYLGDAWLGQVKDL"
     gene            939..1934
                     /gene="fliG"
                     /locus_tag="PKI05_09475"
     CDS             939..1934
                     /gene="fliG"
                     /locus_tag="PKI05_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOA52476.1"
                     /translation="MPGDIRKAAILLMSLPREQAAQVLARLDPKQVEAVTIEIARLGT
                     VTPDEQEQALKEFSQANPAGLTGGKGGLEVAKELIEQALGKAANATLETIKQSIEALP
                     FAFLQKVDSQNLITFLIDEHPQTIALVLSHLNPSQAAEIIRGLPPERQVAVVKRIAMM
                     GQTSPDIIHEVEKGLESRMASVMSQRFENAGGVPAVAEILNVIDRATERHILEALAQD
                     DPDLVNEIRRLMFVFEDITKLSDRDIQTVLKHVDNSQWAMALKGASEELKEKILKNMS
                     SRAATLLKEEMEYLGPVRASNVEQVQQQIVDIIRRLEDAGEITVSLNQEEERLII"
     gene            2099..>2891
                     /locus_tag="PKI05_09480"
     CDS             2099..>2891
                     /locus_tag="PKI05_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOA52477.1"
                     /translation="MMRQYMEAKRACPDALLLFRMGDFYELFYEDAKIAAQTLNLTLT
                     SRDKSDNAAPMAGFPHHQLDSYLAKLLAAGFRVAVCDQVEDPRTAKGLVRREITRIVT
                     AGTVTEEGLLDPKEPNYLAAVVPQDPAGIAWIDLSTGRFYAGIFPASRLADELVRLRP
                     AEVLWPEDATQSVPAIDGMVVTKRPTWAFAAAHTQPILQRHFKGQALEGFGFESQGND
                     LPALCAAGAILDYLNETQRTTLAHIERLLRYRVGDFMEIDESTWRS"
BASE COUNT          682 a          839 c          720 g          650 t
ORIGIN      
        1 gcaaatcctt gacttgaccc agccacgcat cacccaaata catttttccc aattcaacat
       61 tttgcacctg tcccgtgaat tgattctttg cctgccaagt gaccttttcc tctttgatcg
      121 tgaaatctcc agaccgagcc accatttcct tcgccgcttc catgccctgg tcaagccgct
      181 tgattaattc cttgtggtaa acttccagca tttccctcac attaagggca atgtggcgac
      241 acgtgtcctt tccaagggca tcaatctccg ttcgcacctg ctctccaaac cttgacttgt
      301 tgattgcgac caatccggtt gtcacggtcg ccgttagcaa aacaccaatc acagaaaatc
      361 cgacaacttt cgtttgcagc ttcatagtct ttcgtgtctc cctcaaaaaa cccataatct
      421 gctttttact atcaaggctt gttcccccat ttcatgtcgt tacaccgaac catcttgcga
      481 aaccggttca acgggtcgaa acgttcggtc cgccccgttt cgggaagtcg gaacacccgc
      541 ttccctgcgg aaccatagcg tgtgcgcagc gcaacacagt cgcagaatta cgaaattttc
      601 accaggagcg ctcgccagta attccgaaag gtccggattt gtcaaaaaga acgcccattt
      661 tcccccaatt tctctctgtc ggatatgttt cccaatttcg ctcaccgtta tccccgattc
      721 ggtacgataa ttcgaatagg cgaccaaagt ggtcacgaat atcctttcgc cgtagtgggc
      781 cacccttcgt cgcccaaaaa acagcgcgtt tatcccaagg atcaaatgca gaaggcgact
      841 cagaagctgc tgtttttggg gaaaagactc aattcccaag agatgtgatc caacgccttt
      901 gacaaattta agcaaaattc gcgagaaccg agttccttat gccgggtgac attcgaaaag
      961 ccgcgatcct cctaatgagc ttgccgcgag agcaggcggc ccaggtcttg gcacggctcg
     1021 atccgaaaca ggtcgaggcc gtcaccattg aaatcgcccg gctgggaaca gtcaccccgg
     1081 atgagcagga gcaggcccta aaagagtttt cccaagccaa cccggcaggc ttgaccggtg
     1141 gaaaaggtgg cctggaggtc gccaaggaac ttattgaaca ggccctcggc aaggccgcta
     1201 atgcaaccct ggagacgatt aagcagtcaa tcgaggccct tccttttgca tttttacaaa
     1261 aagttgacag ccaaaaccta atcacgttct taattgacga gcacccgcag acaattgctt
     1321 tggtactctc tcacctcaat cccagtcagg cggccgagat tattcgaggg ctccccccgg
     1381 aacgccaagt ggccgtggtc aaacgcatcg ccatgatggg tcagaccagc cctgatatta
     1441 tccacgaggt ggaaaaaggg ctggagtccc gcatggcgag tgtgatgagt caacgatttg
     1501 aaaacgccgg tggtgtcccc gccgtggccg aaatcctcaa tgtcatcgac cgggcaaccg
     1561 aacgccacat cctggaggcc ctcgcccagg acgatcccga cctcgtcaat gagatccgcc
     1621 gattgatgtt cgtctttgag gacatcacca agctcagcga tcgcgacatc caaaccgtgc
     1681 tcaagcatgt ggacaactcg cagtgggcca tggccctcaa aggcgcaagt gaagagctta
     1741 aggagaagat actcaagaac atgtcaagcc gggctgcaac cctgcttaag gaggaaatgg
     1801 aatatctcgg cccagtccgt gcgtcaaatg tggagcaggt ccagcaacag atcgtggaca
     1861 tcattcgtcg cctcgaagac gccggcgaga tcaccgtcag tctcaatcag gaagaagaac
     1921 gactgatcat ctgatcgcat ctaattcgtc tcagcgatgg gccaagaatg tgtactccgt
     1981 ggggccttct tgtgccacgg tctttatcat aatcatatag aagattgtgg ccctttttca
     2041 taataacgtg tctgatgcgg ttttcgcgtt tgccctgatg agtcaagact taacccccat
     2101 gatgcggcag tatatggagg cgaagcgggc gtgtcccgac gcgctcctcc tgttccgcat
     2161 gggtgacttc tacgagctgt tttatgaaga cgcgaagatt gctgcccaga ccctcaacct
     2221 tacgctcacc agtcgcgaca aaagtgacaa cgccgcgcct atggcggggt ttccgcatca
     2281 tcaactggat tcctacctcg ctaaattgct ggcggcgggg tttcgcgtcg ccgtttgcga
     2341 tcaggtagaa gacccgagaa cggccaaggg tctagtccgg cgcgagatca cgcggattgt
     2401 tacggcagga actgtcaccg aagaggggct gctcgacccc aaggaaccga actatctcgc
     2461 tgccgttgtc ccgcaagacc cggcagggat tgcctggatc gacctttcca cggggcggtt
     2521 ctatgccggg atattccccg ctagtcggct cgctgatgaa ctggtccgtc tgcgaccggc
     2581 cgaagtcctt tggccggagg atgctacgca aagcgtgcct gccattgacg gcatggttgt
     2641 cacaaaacgg cctacgtggg cctttgctgc cgcccacacc cagcctatcc tgcagcgaca
     2701 tttcaaggga caagccctcg aaggctttgg ctttgagtcc caagggaacg atctgcccgc
     2761 cctttgtgct gctggggcca tcttagatta tctcaatgag acgcaacgga ccaccctggc
     2821 acatatcgaa cggttactcc gctaccgagt cggagatttt atggagatcg acgaatcgac
     2881 ctggcggagt c
//
LOCUS       DAOPTR010000471         2890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_27593_length_2890_cov_3.516755, whole genome shotgun sequence.
ACCESSION   DAOPTR010000471 DAOPTR010000000
VERSION     DAOPTR010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27593_length_2890_cov_3.516755"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            243..1055
                     /locus_tag="PKI05_09485"
     CDS             243..1055
                     /locus_tag="PKI05_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52478.1"
                     /translation="MKYRVVVMAAGALLMMGMVAETASAGLLSRRPGLFQRLACRRVS
                     PAVAALQQERAKLEQAKQEAEEAKAAAEAAKKEAEAVKEAVEKALAAVKEEAAKALAQ
                     IRQETEAALKAKAEAEAASAKAKADVAAAEKAKAEAADLTAKAKALMEQAQAEMAKVE
                     AAKADLEKARQALQAEQQKLEAAKEDLAKQQAAVEEARKQAEALKAEAEKIKAEATKP
                     AEPTGQEAAPPSNESAAPAQSTQSAENASGAEAGSAGASAQGSPDTSTAATQ"
     gene            1476..2333
                     /locus_tag="PKI05_09490"
     CDS             1476..2333
                     /locus_tag="PKI05_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOA52479.1"
                     /translation="MKLVTYKGPLGPRVGILREGKVLDLQQNEPELPTDIIALLRMGE
                     RAKPLLERVLQRGTVLETEVKRLAPIPHPQKVICVGLNYSDHARETGATPPPEPVIFN
                     KFPTAIIGPEEPIRLPRESANVDYEAELVVVIGRAGRRIPENEALDYVAGYMCGNDVS
                     ARDWQMQKPGGQWLLGKSFDTFAPTGPYLVTRDEISNPHALGVRLILNGQVMQQSNTN
                     QFIFNIPQLIAYVSQVCTLEVGDLLFTGTPGGVGFARKPPVFLRDGDVVEVEIEGIGT
                     LKNVVVADR"
     gene            complement(2467..>2890)
                     /locus_tag="PKI05_09495"
     CDS             complement(2467..>2890)
                     /locus_tag="PKI05_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase"
                     /protein_id="HOA52480.1"
                     /translation="DGYIWVMGRIDDVLNVSGHRLSTIEVESALVSHPAVAEAAAVGR
                     PHEVKGEAICAFVTLAEGYEPSEQLRQELKNHVRKEIGALAVPDDIRFTTALPKTRSG
                     KIMRRLLRDIAAGRETVGDTSTLEDYSVLARLRAGEEG"
BASE COUNT          647 a          781 c          882 g          580 t
ORIGIN      
        1 gggaaagtta gggcataccg ccgggcaatt ccgcgtgcgt catgttttcc ccagagtggg
       61 ggcaataacc cagcccctgt ttatttgccg acccacatat tcgataattt tggcagccta
      121 cgttctgatg gtaagtagcc tgcatctgag gttaactaag tagtgcgaag ccccttggtg
      181 atgttgaggg tggtcctcac agatggcttc gccggggttt tgattgaaaa ggaggacacc
      241 gcatgaagta cagagttgtc gtcatggcgg cgggggccct tctcatgatg ggaatggtcg
      301 ccgagaccgc ctccgcagga ctgttgagcc gacgccccgg cctgtttcag cggttggcgt
      361 gccggcgggt cagtccagcc gtagccgctc ttcagcaaga gcgggctaag ttggagcaag
      421 caaagcagga ggctgaagaa gctaaggcag cggcagaggc cgccaagaag gaggccgagg
      481 ccgtgaaaga agccgtcgaa aaggcattgg ctgccgtgaa ggaagaggcc gccaaggcgt
      541 tggcccagat tcgccaggag acggaagcag ccctgaaggc gaaggccgag gccgaggctg
      601 cttccgcaaa ggccaaagcc gacgtggctg cggcagaaaa ggcgaaggcg gaagctgctg
      661 atctcaccgc gaaggcaaaa gcgctgatgg aacaggccca ggccgaaatg gcaaaggttg
      721 aggccgccaa ggcagatttg gagaaggccc gccaggcact ccaggcagag cagcaaaagt
      781 tagaagctgc taaggaggac ttggcgaagc agcaggccgc tgtcgaggag gcgcgcaaac
      841 aggccgaggc cttgaaagcg gaagccgaaa agatcaaggc tgaagccaca aagccagccg
      901 aaccgacggg tcaggaggct gcccctccat cgaatgaatc agcggcacca gcccagtcaa
      961 cccaatcggc cgaaaatgcc tccggcgccg aagctggctc tgcgggggct tcggcgcagg
     1021 gatcgcccga cacgtcgacg gcggctaccc agtgaagcat ctttgatcta aaccgtcggc
     1081 atcgggacta tcccgcagga ccggtctgga acacgctgtt tcagaccggt tttttgttgg
     1141 tgccgtcgat cgtgagcgaa agattactgc aacttgtgat tgtgcacgtg tcgcaggagg
     1201 actgcagaat gtcagacgag ccgttatact catttgatta ctcaacggat ggatgcctca
     1261 ccattaagag cctatcttat aatctcccct ctcccgttcg gcgggagagg ggtcgggggt
     1321 gagggtggct cgattcgctg ccggttttct cgatcgattc cgacgatcgc ttgccctcac
     1381 cccaaccttc tcctggaggg agagggggac tttctgggat agactctgag tgaaatgatc
     1441 cggatattgg taaccgaaac cagggagaag atgtcatgaa attggtcacg tacaaaggtc
     1501 ccctcggtcc gcgcgtgggg attctccgcg aagggaaggt gctcgatctt cagcaaaatg
     1561 aaccggaatt gccaacggac ataattgctt tgcttcgtat gggcgaacgg gcaaagccgc
     1621 tcctggaaag ggtacttcaa cgaggtaccg ttttggagac cgaggtaaaa cggttggccc
     1681 ccattcctca tccacaaaag gttatttgcg ttgggttaaa ttacagtgat cacgcacggg
     1741 aaacaggggc gacgcccccg ccggaacccg tgatcttcaa taagttcccc acggcgatca
     1801 tcggcccaga agaacccatc cgtttgcccc gagaatcggc caacgtcgat tacgaagcgg
     1861 aactggtggt ggtaattggc cgggccggac ggcgcatccc cgagaacgag gccctcgatt
     1921 acgtggccgg ttatatgtgc ggcaatgatg tgtcggctcg agattggcag atgcagaagc
     1981 ccggaggaca gtggttgctc ggaaaatcgt tcgacacctt tgcgcccacc ggtccctatt
     2041 tggtgacacg tgacgaaatt agcaatccgc acgcgctggg agtgcgactg attctcaatg
     2101 gccaggttat gcaacagtcc aacacaaacc aattcatctt taatatcccg cagttgatcg
     2161 cctatgtctc ccaagtctgc acgttggaag taggagacct tctcttcacc ggtacgcccg
     2221 gcggtgttgg ttttgcccgc aagccaccgg ttttcctgcg ggatggcgac gtcgtcgaag
     2281 tcgagatcga agggatcggt accttgaaaa atgtggtcgt tgcagatcgg tgaaacctgg
     2341 cgagcgattg ccggcaattg gggacctgct tcttcttttt ccaaataaca aaaaacgacc
     2401 cgtgatcgct agaccacggg tcgtttgttc gttcacctcg taaaaggtgt taagctgtgg
     2461 tgggcgttac ccttcctcgc cggcacggag ccgtgctagg acgctgtaat cctccagcgt
     2521 gctggtatcg ccaacggttt cgcgtcctgc agcgatatca cgcaagagcc gccgcatgat
     2581 cttaccactt cgcgtcttgg gtaaggccgt ggtgaagcgg atatcgtcgg gcacggccag
     2641 ggcaccgatt tctttccgca cgtgattctt caactcttgc ctaagttgct cactcggttc
     2701 atacccctca gcgagggtca cgaaggcgca aatggcctcg cctttgactt cgtggggacg
     2761 tcccacagcg gcagcttctg ccacggcggg gtgactgacc agggcgcttt cgacttcaat
     2821 ggtgctgagt cggtgacccg acacattcag cacatcgtcg atgcggccca tgacccagat
     2881 gtagccgtcc
//
LOCUS       DAOPTR010000472         2878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_27760_length_2878_cov_3.369819, whole genome shotgun sequence.
ACCESSION   DAOPTR010000472 DAOPTR010000000
VERSION     DAOPTR010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27760_length_2878_cov_3.369819"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /gene="prmC"
                     /locus_tag="PKI05_09500"
     CDS             <1..707
                     /gene="prmC"
                     /locus_tag="PKI05_09500"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOA52481.1"
                     /translation="GLEVLDQIGGRRRAEGVPVAYLVGEREFFSLKFRVTPDVLIPRP
                     ETEFVLVTLLERARQSGQGGWRICDVGTGSGVLAICAAKHLPDSQILAIDKSPQALAV
                     AKENAIRHHVADRIEWLESDLLEAVPSEMTFDFIISNPPYVSEAEFANLPTTVRNYEP
                     YQALVAGPRGTEVIERLIPQAAEKLVTGGYLIFEISPMICQRVCELVEADGRFRLGPV
                     VRDLARLPRVIQAQRI"
     gene            complement(747..1748)
                     /locus_tag="PKI05_09505"
     CDS             complement(747..1748)
                     /locus_tag="PKI05_09505"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOA52482.1"
                     /translation="MSGRIPLRALSELVSDEENDTFALLSSKEELTTRDGKPYWMVTF
                     RDAQVEFTFPIWCDSPWAAACREQWAVGTFYKLRVVRRETRYGPQLEIHRIRETVDQD
                     RADGFDPLMCLPQSRFPAEELFQRLEALVQKEIKSPSVLQLLVMILHTFRESILTLPA
                     ARSHHHAYVGGWLEHTLSVTETCVFLADKYIQLYPDMKPPLDRDLVIAGAVLHDIGKL
                     RELEFQITTTEYSPEGALLGHIVLGRDFVRDFAPKAGLDSEQQLRLEHLIIAHQRLAE
                     WGSPRPPMTPEALIVHFADDLDAKFHMFWKALQHDSKEGPLTSDRNPLRQAIFRGLK"
     gene            complement(2012..>2878)
                     /locus_tag="PKI05_09510"
     CDS             complement(2012..>2878)
                     /locus_tag="PKI05_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52483.1"
                     /translation="PPPVYRLNPQPPFDLFAGEQLALVGRYRKSGRAEVVLKGRIDDE
                     QKEFRFPVDFVARSGDESKAFVEKLWAIRRVGDILDEIDLHGKNPELIDELTRLAMKH
                     GIVTPYTSFIANENVPIHDLTQSRRETESRLRALEAAEGYGGFAQRSFKGRMQQAQAA
                     DAASALRSFGGLGAEFAAPSAAAPGGAGMMGGVAASAPALAPAEEEIARAAAGETMRY
                     VGNRVFYKRQGQWIDGTLKPEQQQNPIRIKQFSEEYFNLVRQYGRQVSQYLVFDEPTL
                     VNINNQAYLIEP"
BASE COUNT          634 a          794 c          816 g          634 t
ORIGIN      
        1 tcggcctgga ggtgctcgac cagattggcg gtcgtcgccg agccgaaggc gtgccggtgg
       61 cttatctggt tggggagagg gagtttttct ccctgaagtt tcgggtcaca cccgatgttc
      121 tcattccgcg accggagacc gagtttgtcc tcgttacctt gcttgagcga gcgaggcaat
      181 ctggccaggg cgggtggcgg atttgcgatg tgggaactgg ctccggggtc cttgccattt
      241 gtgcggccaa gcatttgccg gacagtcaaa tcctggccat cgacaagagt ccccaggccc
      301 tggcagtggc caaggaaaat gcgatccgtc atcacgtggc cgatcgaatc gaatggctgg
      361 aaagcgatct cctcgaggcg gttccgtctg agatgacttt cgatttcatc atttccaacc
      421 cgccctatgt cagcgaggca gaatttgcga atctgccgac caccgtgcgc aactatgaac
      481 cttatcaagc cttggttgcc ggaccgcgag gtacggaggt tattgagcgg ctgattcctc
      541 aagcagcgga aaagctggta acagggggct atttgatctt tgagatttct cctatgatct
      601 gccagcgggt atgcgaattg gtggaagccg acgggcgatt ccggttgggg cctgttgtcc
      661 gtgatctggc gcgtctgccc cgcgtgatcc aagcccagcg gatataagta gaatagaacc
      721 cgccgccaag gatatggggc tcagagttac tttaaacccc gaaaaatggc ctgccgcagg
      781 ggattgcgat cgctggtcag aggaccttct tttgagtcgt gctgaagtgc cttccaaaac
      841 atgtggaatt tcgcgtcgag gtcatcggcg aagtggacga tgagggcctc cggcgtcatg
      901 ggcggccgtg gagaacccca ttccgctagc ctttgatggg cgatgatcaa gtgttccaga
      961 cgaagttgct gctctgaatc aaggcccgcc ttgggcgcga agtcacggac aaaatcccgt
     1021 ccaaggacga tatgtccgag caacgccccc tctggcgaat actctgttgt ggtgatctga
     1081 aactccaact ctcgcagttt cccgatgtcg tggagaacgg cacccgcaat gacaaggtcg
     1141 cggtcaagag gcggtttcat gtcgggataa agttggatgt atttatcggc gagaaacaca
     1201 catgtctcgg tgacggacaa ggtgtgttcc aaccaacccc caacataggc atgatggtga
     1261 ctccgtgcgg ccgggagagt aagaatcgac tcgcgaaacg tatggaggat catcacgagg
     1321 agctggagca cggacggcga tttgatttct ttttgaacaa gtgcctcgag acgttgaaag
     1381 agttcttcag cgggaaaacg cgattgaggt aggcacatca ggggatcaaa accgtccgct
     1441 cggtcttgat ctacggtttc tcggatgcga tggatttcga gttgaggacc gtatctcgtc
     1501 tcgcgacgaa caacccgtag tttataaaac gtccccactg cccattgctc gcgacacgcc
     1561 gcagcccagg gagaatcgca ccagatcggg aaagtaaact ccacctgggc gtcacggaag
     1621 gtgaccatcc agtacggctt gccatcgcgg gtggtcagtt cctctttgct actgagcagg
     1681 gcaaaggtat cgttttcctc atcgctaact aattcactta atgccctgag cgggatgcgt
     1741 cccgacatag ttgtcacctc atcaattcac accatacccc tttttctcat gatgcctgcg
     1801 ataagttcca aatcagccgc aaatgtacgc aagaagaaag ccgttgccaa gaggaccgag
     1861 aagacagttt cacgttttcg cattttcttt atcgacaatc acgacaccgg ctgcgcataa
     1921 aaagggacgc gccgaagcac gtccccacaa ctagtctggc ccgagcaacg ttgtccccaa
     1981 ggaacgtgac cgatgctcct gacctgttat gttaaggttc aatcaagtaa gcctggttgt
     2041 tgatattgac gagcgtcggt tcgtcgaaca caaggtattg tgagacttgc cgcccgtact
     2101 gacgcaccag gttgaaatac tcctcgctga attgcttgat gcggatgggg ttctgctgct
     2161 gttctggctt tagtgtgcca tcgatccatt gcccttggcg tttgtagaag acacggttac
     2221 caacgtaccg catcgtttcc cctgcggcgg cacgagcaat ttcctcctca gcgggcgcca
     2281 acgcgggggc actggccgcc acaccaccca tcataccggc gccaccaggt gccgctgcgg
     2341 agggggcggc gaactcagcc ccgaggccgc cgaatgagcg aagcgccgag gccgcatccg
     2401 ctgcttgggc ttgttgcatt cgccccttga atgaccgctg ggcgaaaccg ccatatccct
     2461 cggcggcttc taaggccctg agtcgtgatt ccgtctcacg gcgcgactga gtcaaatcgt
     2521 gaatcggcac attctcgttg gcaatgaagg atgtgtaggg cgtcacaata ccatgcttca
     2581 ttgccaatcg agtcagttcg tcaatcaact ccgggttctt cccgtgtagg tcaatctcgt
     2641 caaggatatc acccacgcgc cgaatggccc aaagcttttc cacgaaggcc ttcgactcat
     2701 ctccgctccg ggcgacaaaa tcgacgggaa aacggaactc cttttgctcg tcgtcaatcc
     2761 ggcccttgag aacgacctca gcgcgacccg acttgcgata gcgtcccacc aaggcaagct
     2821 gctcaccagc aaataaatcg aacggcggtt gcggattcaa acgatagacg ggcggagg
//
LOCUS       DAOPTR010000473         2878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_27765_length_2878_cov_2.727949, whole genome shotgun sequence.
ACCESSION   DAOPTR010000473 DAOPTR010000000
VERSION     DAOPTR010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27765_length_2878_cov_2.727949"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..1324
                     /locus_tag="PKI05_09515"
     CDS             194..1324
                     /locus_tag="PKI05_09515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA52484.1"
                     /translation="MKTVHVIPTLDRAGAEKQMVYLATGLKARGVDVHVCVLTRDGPY
                     REDLERAGIPITLIGKRGKIDLFALRHLQWVLTEMRPDIVQTWLFAANVYGRFAARRA
                     RVPHIIASERCVDLWKKPWHFWIDRRLARVTDRIIVNSSGVRDFYVQRGISPEKIEVI
                     PNGIVVPQRDTTPRQSILAELGLPQDARLIGLVGRLWPQKGIRDAIWAADLLKRIRND
                     VHLLIIGDGPEREALIRYRNSIEIQDRVHFLGHRNDVLRIMPHFDALWNTSRYEGQSN
                     AILEAMTLKVPVVASDIPGNRDLVVNEVTGFLVPLGPHFRAGVARFTKKILEDPDLAR
                     RLGEAGYQRVLTEFTVEQMVIRHEKVYQELLAGSPARDFRIA"
     gene            1346..>2878
                     /gene="asnB"
                     /locus_tag="PKI05_09520"
     CDS             1346..>2878
                     /gene="asnB"
                     /locus_tag="PKI05_09520"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOA52485.1"
                     /translation="MCGIAGGVWYEPTAAISAATLQRMTEVLQHRGPDDQGFYLKEIA
                     ADGHAPACGVALGHRRLSIIDLAGGHQPMSNEDETLWIVFNGEIYNFRALRQELEAAG
                     HRFRSRSDTEVLLHLYEEKGLHFLEFVNGMFGLALWDEPKRRLILARDRLGEKPLVYY
                     ADSQRLLFASELKALMEVPGVPREIDPFAVDAYFLYQYVPHPWTIYRGIKKIPPATMA
                     IYEPKNLTLHRYWDPDFSRVENGMALEEIKEELRSRLEKAVTMRLEADVPLGAFLSGG
                     IDSTIVVGLMQKNSREKVRTFSIGFPVAEYDETHYARIAAEAFGTEHREHRVNPDAIG
                     VLPSLVWHYDEPFADSSAIPTWYVAEKTRQHVTVALTGDGGDELFCGYTRYWATAFAE
                     GIASLPRWIRWIFNERLWRWAPRTRQRSFFGRVHRFTESLPLATPQRYIDWIAIFNRA
                     RRLALYTPEFQEQLTAVPEDFFLEIYSRFASRDIVSAISLADLFSYLPCDLLVKVDIA
                     AMA"
BASE COUNT          625 a          750 c          817 g          686 t
ORIGIN      
        1 ctcgcacaga acggtgggtg aggcgagacc cacttagcta ccaagacggc aagacgcatc
       61 actaccgtac gcggggataa tgtaattgac tatttccaga aacgatcaac ctgtaaatgt
      121 cagtcgccca gcctgattgg cgtgatcatc aacaagctac tagcgttgca gaatacgctt
      181 gatattgaag ccgatgaaaa ctgtccacgt cattcccacc ctggatcgtg ctggggccga
      241 aaagcagatg gtctatctag ccacggggct gaaggcccgg ggcgtggatg tccatgtctg
      301 cgtcttgacc cgtgatggtc cgtatcgaga agaccttgaa cgggctggga ttcccatcac
      361 gttaattggc aagcgaggca agattgattt gttcgctttg aggcatctgc aatgggttct
      421 cacagaaatg cgacccgaca ttgtgcaaac gtggcttttc gcagccaacg tttacggtcg
      481 gttcgcagca cggcgggctc gcgttcccca tatcattgcc agcgaacggt gtgtggacct
      541 ttggaagaaa ccatggcatt tttggattga tcgccgtctg gcacgagtca ccgatcgcat
      601 cattgtgaac agttccggcg tccgggactt ttatgttcag cgtgggattt caccagagaa
      661 gatcgaagtt attcccaatg ggatcgtagt tccccagcgg gataccacgc cgcggcagtc
      721 gatcctcgca gaattggggc tgccgcagga tgcccggttg atcggattgg ttggacggct
      781 ttggccgcag aaaggtatcc gcgacgcgat ttgggctgcg gaccttttga aacggattcg
      841 caatgacgtc catctcctta tcattggcga tggcccggag cgcgaggcct tgattcggta
      901 tcgaaacagt attgagattc aagatcgggt acactttttg ggacaccgga atgacgtttt
      961 gcgaatcatg ccccattttg acgccctatg gaatacgagc cgttatgagg gacaatcgaa
     1021 cgcgatcctg gaggccatga ccctgaaggt ccccgtggtg gcatcggaca tcccagggaa
     1081 tcgggacctg gtggtaaacg aggtgacggg ctttcttgtg ccattgggtc cccattttcg
     1141 ggccggagtt gcccgattta cgaaaaaaat cctcgaagat cccgacctgg cccgccgcct
     1201 gggtgaagca ggctatcagc gtgtgctcac agaatttacc gtggaacaga tggtaatccg
     1261 gcacgaaaag gtgtatcagg aattgttggc cggctctccg gcgagggatt tcagaatcgc
     1321 atgaatgtaa gagcgaggtg actccatgtg tggaattgcc ggtggcgttt ggtatgaacc
     1381 caccgctgcg ataagtgctg caacgcttca gcgaatgacc gaggtgttgc agcatcgcgg
     1441 tccagatgat caaggctttt acctgaaaga gattgcagcc gacggtcatg ctccggcttg
     1501 tggcgtggcc ctagggcacc gacgattgtc catcatcgac ctggccggtg gtcatcagcc
     1561 gatgtccaac gaggatgaga cactttggat tgtctttaat ggtgaaatct ataatttccg
     1621 ggcgttacgg caagagttgg aagccgccgg tcatcgtttt cgcagccgtt ccgatacgga
     1681 agtcctgcta catctttacg aggaaaaggg acttcatttt ctcgaattcg tcaatggaat
     1741 gttcggatta gccctgtggg acgagcccaa gcggcgtctc attttggcgc gggatcgctt
     1801 gggggaaaag ccccttgtct attatgcgga ttcccaacgg ctactcttcg ccagcgaact
     1861 aaaggccctc atggaagtac ccggtgttcc ccgggaaatt gatccttttg ccgtcgatgc
     1921 gtactttctc tatcagtacg tgccccatcc gtggacaatc tatcggggaa tcaagaagat
     1981 tcctcccgca acgatggcga tctacgagcc aaaaaatctt accctccatc gctattggga
     2041 tccggacttc agccgcgtcg aaaacggaat ggcgcttgaa gaaatcaagg aagaactgcg
     2101 ctcgcgattg gaaaaggccg tcacgatgcg gttggaggcc gatgtgccgc tcggggcgtt
     2161 cctctccggg ggcatcgact cgacgattgt agtgggctta atgcaaaaga actcccggga
     2221 gaaggtccgg accttttcca ttggctttcc tgtggccgaa tatgacgaga cccattacgc
     2281 gcggattgcg gccgaggcct ttgggactga acatcgagag catcgcgtca atccagatgc
     2341 gatcggagta ttgccttccc ttgtttggca ctacgatgag ccgtttgcgg acagttccgc
     2401 cattcctacg tggtatgtgg cagaaaaaac ccgacaacat gtgacagtgg ccttgacggg
     2461 tgacggcggc gatgagctgt tctgcggtta cacgcggtat tgggcaaccg ccttcgcaga
     2521 aggcatcgcg tccttgccgc gttggattcg ttggatcttt aatgagcgac tatggcgttg
     2581 ggcaccccga acgcgtcagc gatcattctt tggtcgagtc caccgcttta cagaatctct
     2641 gccccttgca acaccacaac gctacattga ctggattgct atcttcaacc gagcccgccg
     2701 cctggccctt tacaccccag agtttcaaga gcagttgacg gccgtgccgg aggatttttt
     2761 tctggaaatc tactctcggt tcgcttcgcg ggacattgtt tctgccatta gcctggccga
     2821 tctcttctcc tatttaccct gcgacctcct agttaaggtt gatatcgcag cgatggcc
//
LOCUS       DAOPTR010000474         2877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_27777_length_2877_cov_3.818923, whole genome shotgun sequence.
ACCESSION   DAOPTR010000474 DAOPTR010000000
VERSION     DAOPTR010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27777_length_2877_cov_3.818923"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(315..1253)
                     /locus_tag="PKI05_09525"
     CDS             complement(315..1253)
                     /locus_tag="PKI05_09525"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HOA52486.1"
                     /translation="MASAVFGALVLVTLVWWAVVFTSRQTRQMLWPRYRFRQLPWRIR
                     DLLLVWLLFLLALAVSTVVSPLSTAKEGARVYQNEPQRHGLDSPGRDEDETTQGQHWV
                     LVLIQSDRSLKTVLLCLLGTGILGPIAEEILFRGFLQGWSHRVEWEHRRTRLNIAPIR
                     RQLPLGAASVALVAVLFATVHYRPDEPSPSVDVIRVILIRQTLAYSLFLPVALAIVAL
                     KDKPRGLRLIYDRRWFFCDITLGAGWFLAWIVPVYFVQGALKTILPWKAMADPCTMIV
                     IGTILGTLFARTGRLAASIVFHVLLNITSLSLAFLM"
     gene            complement(1264..1890)
                     /gene="pgsA"
                     /locus_tag="PKI05_09530"
     CDS             complement(1264..1890)
                     /gene="pgsA"
                     /locus_tag="PKI05_09530"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOA52487.1"
                     /translation="MSKMIAGTSPRWFNLPNCLTLLRILLAVALFVTLSIHERVGYGV
                     SIGLFIVAAATDWLDGFLARRLNLVTALGRILDPFADKLIICGTFVFLAATPKMIETP
                     WGLHPWIVVVVISRELLITGLRSFLEGQTVDFSAKFSGKLKMGFQCITAVVAMMYLAD
                     TGYNTHLSYLLWLALVVSTYLTLLTTVYSGIAYIMIGWKKVRDLEAAG"
     gene            2157..>2877
                     /locus_tag="PKI05_09535"
     CDS             2157..>2877
                     /locus_tag="PKI05_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOA52488.1"
                     /translation="MPQWKGLERTIRANEPLAMHTWLGVGGPAEFYAEPRNADELADI
                     VRCAYEQGIPIRVLGSGSNILIRDEGVPGLVIQLSHPYFADVTIDGNIVSAGGGASLS
                     RVITTAVHEGLAGLEVLTAIPGTIGGALKGNVGAHGGDIGQWVKEVTVLDYTGQLSVK
                     DRTEVVFGYRRSSLDDLIILQAKFELEPEDPHELARRLQKLWIVRKAAQPMGHQASVR
                     VFRDPRGTTAAEVVEAAGLKGT"
BASE COUNT          769 a          744 c          769 g          595 t
ORIGIN      
        1 gcgtgaaatt ttgagatcaa cccacaacca acgataccga agcccaaggc catctcacgt
       61 ctccttcttc aatttaaacc aatttctcgc tttggttttt ccagtaaatc tcgacaaacg
      121 cccattagtg ctctgttgcg gcgaggattt cgggctgaat caatctgaaa aaggactgac
      181 gcgattatac ggaatcgccg cctcccgtta aagtcactca ccctccgttt cagtgctttt
      241 cgagccggtt cccaattggc caatgttgaa acgttcgaac gcattcgtct atttcctaac
      301 gcaaatcccg cgaatcacat cagaaaagcc aaagataggc tcgtgatgtt caagagaaca
      361 tgaaagacga tactggctgc tagccgcccc gttcgggcaa agagtgtccc aaggattgtg
      421 ccgatgacga tcatggtaca tggatcggcc atggccttcc aaggcaggat cgtcttgaga
      481 gcaccttgca cgaagtacac tgggacgatc cacgccaaaa accaaccggc tccaagggtt
      541 atgtcgcaaa agaaccaccg ccgatcgtaa atcaaccgta gtcctcgcgg cttgtcttta
      601 agtgcgacaa tggccagggc aaccggcagg aacagcgaat aagcgagtgt ctgtcgaata
      661 aggatcacgc gaatcacatc aacactcggg gagggctcgt ccggccgata atggactgtt
      721 gcgaaaagaa ccgcaaccag tgcaacggat gctgcgccca aaggtaattg gcgtcgaatg
      781 ggtgctatat tcaagcgggt gcggcgatgt tcccattcca cacggtggct ccatccctgg
      841 aggaaacccc gaaagaggat ttcctccgca atcggcccga ggattccggt gcccagcaaa
      901 cacaagagga ccgttttcag cgagcggtcg gattggatca gaaccaagac ccagtgttgg
      961 ccctgggtgg tttcgtcttc gtcacggccc ggcgaatcta ggccgtgtcg ttggggctca
     1021 ttctggtaaa cgcgcgctcc ttcttttgcc gtggaaagag gggagacgac agttgaaaca
     1081 gcgagagcca aaaggaacaa aagccaaacc agtaaaagat ctcgaatacg ccagggcaat
     1141 tgtcgaaagc ggtagcgagg ccaaagcatc tggcgggtct ggcgggacgt gaaaacaaca
     1201 gcccaccaaa ccaaggtgac tagcaccaac gcaccaaata ctgctgaagc catacaaaga
     1261 gattcatccg gcagcttcga gatcccgaac cttcttccaa ccaatcatga tataggcgat
     1321 accggaataa accgttgtca ggagcgtcaa ataagttgac acaaccagcg ccaaccatag
     1381 aagataggac aagtgcgtgt tgtaaccggt atctgccaaa tacatcatgg ctacaacggc
     1441 cgtgatgcat tgaaagccca ttttaagttt tcccgagaat ttggctgaaa aatccaccgt
     1501 ttgcccttcc agaaagctcc tcagaccggt gatgaggagt tcacgactga tgacaacgac
     1561 cacgatccat ggatgaagtc cccagggagt ttcgatcatc ttgggggttg cggccaagaa
     1621 aacgaatgtt ccgcagatga tcaacttgtc ggcaaatgga tcaagaattc gaccaagtgc
     1681 cgttacgagg ttcaaacggc gtgccaaaaa accgtcgagc cagtccgttg cagcggccac
     1741 aataaataac ccgatggaca cgccataccc aactcgctcg tggatggaca gcgtaacaaa
     1801 aagagcgaca gctagaagga tcctcagtaa tgttagacaa ttaggcaaat tgaaccagcg
     1861 aggagatgtc ccagcaatca tcttgctcat gactcgattc tagatggatt acgcctgaaa
     1921 tacctggcaa taaccgggcc gctctaacgg accagccaag gtagatgtgt ccttgcgaca
     1981 ttaacgacga aataccctct tatcgtaacc gaaaccaagc ggccgtaaca ctccgtgagg
     2041 aggtgtaacg tttttaggcg ggattcggcg cgagattctt cgagtcgcca gacggcgagc
     2101 acttcggtaa aatcataggc tgtgggcaag gattgtaccg gattgaggag ctgacaatgc
     2161 cgcaatggaa aggtctggaa cgaaccattc gggccaatga accactagcc atgcacacgt
     2221 ggcttggggt tggaggtccc gcagagtttt atgctgagcc tcgtaacgct gatgaactgg
     2281 ctgacatagt gcgatgtgcc tacgaacagg ggatccccat tcgagtcttg ggaagcggtt
     2341 caaatatact gatccgggac gaaggcgttc cggggttggt catccaactg agccatccct
     2401 attttgctga tgtcacaatt gacggaaata tcgtttctgc tggtggtggt gctagtctct
     2461 cacgggtaat cacaacggct gttcacgagg gtttggccgg cctggaagtg ttgactgcta
     2521 tccctggtac cattggtggt gctctcaaag gaaatgtggg agcccatgga ggggatatcg
     2581 gccaatgggt gaaggaagta accgtgctgg attatacagg ccaactctcc gttaaagacc
     2641 gcacggaagt cgttttcgga taccggcgaa gcagtcttga tgatctcatc atcctacagg
     2701 ccaagtttga acttgaaccg gaggacccgc atgagctggc tcgccgcctc caaaaactgt
     2761 ggattgtaag aaaggctgcc cagcccatgg ggcaccaagc gtcagtgcgg gtctttcgag
     2821 acccccgcgg caccacggcg gccgaggttg tcgaggcagc gggccttaaa ggtaccc
//
LOCUS       DAOPTR010000475         2874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_27817_length_2874_cov_3.159986, whole genome shotgun sequence.
ACCESSION   DAOPTR010000475 DAOPTR010000000
VERSION     DAOPTR010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27817_length_2874_cov_3.159986"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(719..1102)
                     /locus_tag="PKI05_09540"
     CDS             complement(719..1102)
                     /locus_tag="PKI05_09540"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOA52489.1"
                     /translation="MGLSSRGLLGSRSSSTYARSRTSSRYGSRSQTQRDVLRSTVRVA
                     FNPPVENSEQIAAELAARLSRIPGLKNAPQAANVQVDLEGRTAILKGTVPTANDRLVL
                     ERLLLLEPGVDKVQNELVVAGSNPQ"
     gene            complement(1906..>2874)
                     /locus_tag="PKI05_09545"
     CDS             complement(1906..>2874)
                     /locus_tag="PKI05_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52490.1"
                     /translation="HVKPAKQIQQEMAVIIAHGGRYFAWDNPTPESGLRPERYRMMAD
                     VVVPFLRSRQAWCLGTTALPDVALFHGAAAHYFLNEVTPVSFPRNNPPLLTACEALRR
                     LHLTPEMISDRRLENGDIRAKLLIFEDTPLLTDENRQGLRRYLENGGRVLLTGRAVSQ
                     SKLWDSGTLSAIEHRNIGRGEVYFLPSVLFAGEEGSTSQSSVQILDRMLPSKERRLVT
                     DAPSTVEVTLRRRGNEMIVHFVNIAAGKRARDPATNPFVALRVEELPPAPACRVSVAL
                     PGQPQSIVLQPQAAEIRMWKHESGRVEFEVPGFHVHQMAVIKLKGE"
BASE COUNT          662 a          879 c          729 g          604 t
ORIGIN      
        1 ccagtgcgga agtttgtctt caccaacgag cagctccaac aagtttactc cgagtcgaaa
       61 cttggcccct cttataactt gctcatcccc tgggaggagt tctcgcccaa gtcacgtcgg
      121 gtcagcttaa tcgtccgctt tatcccggag aatggcccgt cggtcgttag tgaacaagtc
      181 aaggtcttct tgcccgggtt ggatgaatcg atgctggcag gcaactcccg tgcggcaagt
      241 gacaggcaac cgtcgccaaa ttctaactcg gcagaccctt ccttggatgc cgcagatggc
      301 tcacgaacgg ccgtcacaca gtggcctata agtcctacaa gctatcagga atctgtgaaa
      361 caggcacagc ctgaaagtag gatgcaagat aaaggagtat cctcatcctc cggagtgacc
      421 acgcccgaga agaaacgtcg catggagacg ctcaccttga acatatcgcc gcggttagga
      481 aacggggtac cgtcccgcct gggccacggg gatacttctc tctcaaccgg tgggaatcat
      541 gaaggggctc agtcccaaca gaccagcccg gttggccacg cctcgccaaa taccccgggt
      601 gcttcagctt ctgcacaaac gcaaagccaa gcgaacctga gctctctgaa tatccagacc
      661 cctgaacagg cgtacctgac caccgtgtct gccaaaattc cccgtagccc cggggaactt
      721 attgcggatt tgaaccggca accaccagct cgttttgcac cttatcgacc cctggttcca
      781 gaaggaggag tctctcaagg actaaccgat cgttcgccgt cggcactgtc cccttaagaa
      841 tggccgtccg accttcgagg tcaacttgaa cgttcgcagc ctgaggtgca ttcttcaagc
      901 cggggatgcg ggacaagcgt gccgccaatt ccgcggcaat ctgctccgag ttttcgacag
      961 gcgggttaaa ggccacccgg acagtggaac gaagaacatc gcgctgggtt tgactccgtg
     1021 acccataacg ggaactcgtc cgcgatcgtg cgtaagtgga actgctcctc gaccccagaa
     1081 gccctcgact acttaagccc atcaagctgc tactccctaa ggtcgttcgc ccggtaaggc
     1141 cggtggtcgt ccttcctgta agcccagtcg tcctcccaga actagagcct tgcagggcac
     1201 tcaaggggtt gactatgtct gcgctatccg cccccacgaa cgaactagac ccgaagaact
     1261 gttcggcttg attgactgat tgaaaattca ggaactggcc gctggacatc gtcgaaagcc
     1321 cactggagcc taacgagcta ctccgacttg tgcctgaaat ccgggtacta ctttgcccgg
     1381 tgagactcga cccaaaactc tgcgtcccaa aaagtccact ggaatagctc gtttgcgctc
     1441 tagacgtttc catggcgcca aatagagcga gagccaaccc cgccacgacc gccagaaacc
     1501 gtgcccggag cctggtcatt ttaaaaccct cctcaccccc gtgtgtccgg tgtaacgaat
     1561 cactgcttta atttgctccg cgagatgatc gccgtccgaa gacgttatcg gcacccccag
     1621 cccatcagcc tgcaaaatca ttcgccatta ttataaacgc tccgcacctt gaaaaatccc
     1681 accgaaatcg taggctcgtg gcggatttct ccccatatcg agcggaaacc accccatttg
     1741 ctagaataat gccccctttt tgacgtatgg cgcggaatga gccttcttcc tagcccatgt
     1801 gtcaacaatc cgcgcgtcaa taaatttcgt gctcaaggat agtttgacta tcagttgcat
     1861 tacccctgcc ggacttaatt ccaaggctcg ccaaactcgg tgtaatcatt cccctttgag
     1921 cttgatgaca gccatctggt ggacgtgaaa tccaggcact tcaaattcca cccggccaga
     1981 ttcatgtttc cacattcgga tttccgctgc ttgcggctga aggactatgg actgaggctg
     2041 gccaggcaac gccacggaaa cccgacacgc cggagcaggg gggagctcct ccacccgaag
     2101 ggccacaaaa ggattcgtcg cagggtctct agcccgcttc ccggcggcga tgttcacaaa
     2161 atggacaatc atttcgtttc cccgccggcg aagggtgacc tccaccgtgc ttggggcatc
     2221 ggtcactaac cggcgttcct tggaaggtaa catccggtcg agaatttgga cagacgactg
     2281 acttgtggag ccctcttctc ccgcaaaaag gacactcggg agaaaataca cctcaccgcg
     2341 gccgatattg cggtgttcta tcgccgataa cgttccactg tcccataatt tcgattgcga
     2401 gactgctcgc cccgtgagca gcacccgccc accgttttcc aggtatcgtc gtaagccctg
     2461 acgattctcg tccgtcagta aaggtgtatc ttcaaagatc agcaacttgg cgcgtatgtc
     2521 gccgttttcc aaacgccgat cggaaatcat ttctggggtt aagtgcaaac ggcggagagc
     2581 ctcgcaggcc gtcagaaggg gcggattgtt tcgcggaaag ctcacgggag tcacctcgtt
     2641 caagaagtag tgcgccgcgg ccccgtgaaa caacgccacg tccggcaaag cagttgtccc
     2701 gagacaccac gcctgacgag accgaagaaa cgggaccacc acgtcagcca tcattcggta
     2761 gcgttcaggc ctcagccccg actcaggtgt agggttgtcc caagcgaaat atcggccacc
     2821 atgagcaatg atcacggcca tttcctgctg gatctgtttc gctggcttga catg
//
LOCUS       DAOPTR010000476         2873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_27840_length_2873_cov_2.910930, whole genome shotgun sequence.
ACCESSION   DAOPTR010000476 DAOPTR010000000
VERSION     DAOPTR010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27840_length_2873_cov_2.910930"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PKI05_09550"
     CDS             <1..492
                     /locus_tag="PKI05_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52491.1"
                     /translation="PKRHRGLATTLMMATFDAGLLLGGPVAGITLRLAGQLGWPRYPT
                     MFLVMAGGLLLLAVYFAGTTAANPNFRSRRRRRSHGSQPLPIPREISQQPPVGKISHS
                     TSVATITGHRKAEEELDVKKPVSVTVDQKLAQATVLPALEASSSPTRPILSPAAETVG
                     NPV"
     gene            complement(664..1437)
                     /locus_tag="PKI05_09555"
     CDS             complement(664..1437)
                     /locus_tag="PKI05_09555"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOA52492.1"
                     /translation="MATCLVTGGAGFIGSHIVEALVDRGHKVRVLDNLSTGYLRNLDS
                     VRDQIDFIEGDILDAALVEQVIRGVDWVFHQAALASVPRSVERPLETHAACATGTLIL
                     LDAARKTQVQKFIYAGSSSAYGDQPFSAKRECDLPDPISPYGAAKLAGEYYCRAFYAT
                     YGLPTVVIRYFNVFGPRQDPNSPYSAVIPLFITAILKGNRPVVYGDGLQSRDFTYVAN
                     VVHANLLAAEKEGVADGFSTRRGDGALRCYSSWLPSMRC"
     gene            1735..>2873
                     /locus_tag="PKI05_09560"
     CDS             1735..>2873
                     /locus_tag="PKI05_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HOA52493.1"
                     /translation="MKADELREKYLTFFESKGCVRRPSDVLVPRWDPSVLFTPAGMNQ
                     FKDHFLGRCKLEFTRATTCQKCLRTGDIENVGRTAYHHTFFEMLGNFSFGDYFKREAI
                     LWAWEFLTDKKWLGLDPEKISATVYLDDDEAANIWIQEVGLSPEKVTRMGEDENFWPA
                     NAPSQGPDGVCGPCSEIYYETPWGKVEIWNLVFTQFNRVGPPPNNLRPLPSKNIDTGM
                     GLERTAAVLQGVESNLHIDIIRPIVEAAADVCHVKYDPASDFGRRLRRIADHIRACTF
                     AIHENVTPSPNKQGYVIRRLIRRAVLDGHRMGVREPFFHKLVPVVAEMMKNPYPELQE
                     TIPRVTAVIKGEEENFFGVLETGLDRIERLFEEMERSGRGVVSGA"
BASE COUNT          655 a          808 c          798 g          612 t
ORIGIN      
        1 cccaaacgtc accgcggcct agcaactacg ctcatgatgg cgaccttcga tgccggtctc
       61 ttgctgggcg gcccggtggc cgggatcacg ttacgcctgg ccggacaact cggctggccc
      121 cgctatccca cgatgttttt ggtgatggcc ggggggctcc tcctgcttgc cgtttatttt
      181 gcgggaacca cagccgccaa ccctaatttc cggtcccgac ggcggagacg gtcacacggc
      241 tcacaaccct tgccaattcc acgggaaatc tctcaacagc cgcctgtggg aaagatatcc
      301 cattcaacct ctgttgccac gattaccggc catcgcaagg ctgaggagga gttggacgtg
      361 aaaaagccgg tcagcgtgac tgtggatcaa aaactcgccc aggccacggt acttcccgcg
      421 ttggaagcca gcagtagccc tacgcggcct attctcagcc cggctgccga aaccgtcggt
      481 aaccccgtgt aactttcggt taacagcaga tttgcctgta ataatccacg gaacgcctca
      541 ggccctcgag aaagtcaacc tgcggctcat agcctaggaa cttccgcgcg gcactgatgt
      601 ccgccatgct ttcgcggata tcaccaggtc gaggcggggc aaattcgggc tcaactgacg
      661 tgcctaacac ctcattgagg gcagccaaga gctgtaacag cgtaatgctc cgtccccccg
      721 ccgcgttgaa aacccgtcgg caaccccctc tttttcggcg gctagtaaat tggcgtgtac
      781 gacgttggca acataggtga agtcgcggga ctgaagtcca tcgccgtaca ccaccgggcg
      841 atttcctttc aaaatcgccg tgatgaaaag cggaatgacc gcggagtatg ggctgttggg
      901 atcttgacgg ggtccaaaga cgttgaaata gcggatgacc accgttggta aaccataagt
      961 ggcatagaat gcccgacaat agtactcgcc ggccagtttt gcggcgccat acggcgaaat
     1021 tgggtcaggc aggtcgcatt cgcgtttggc actaaagggt tgatccccat aggcactgct
     1081 ggacccggcg taaatgaact tttgaacctg ggtcttgcgg gccgcatcca agagaatgag
     1141 cgttcccgtg gcacatgcgg catgggtttc caaagggcgc tccacactac ggggcacact
     1201 tgctagggcc gcctgatgga atacccaatc cacgccccga ataacttgtt ccacaagtgc
     1261 cgcatcgagg atatcgccct cgatgaagtc aatttgatct ctgacactgt cgagattcct
     1321 gagataacct gtactgaggt tatccaaaac ccggacctta tgccctcgat ctaccagggc
     1381 ctcaacaatg tgcgatccga taaaacccgc accaccggta actaaacagg tcgccatcgc
     1441 cgtcgtcctc aatttcaagc acatcaaacc gggccaaatg tcccgatgca ttaacgtagc
     1501 atacacctca ttcgttgtgc gattccttcg ttgtggaagt tttctgtgag aaaatggccg
     1561 cgaaggcaac tgaggcaacg gcgctcaatc ttttcgaccg gccaaaaagg gggtaagata
     1621 aggactttgc cactcgtaat gaagcccatc ggcgaaccgc gatggcggtc cgccaagtct
     1681 taacgattgt gccaatctta gcactttccc gaatcaaaag gatgacacat ttttatgaag
     1741 gcggacgaat taagagaaaa atacttgaca ttttttgagt ccaagggttg tgttcggcgg
     1801 ccgagtgatg tccttgtccc ccggtgggac ccgtccgttc tgttcacccc ggccggcatg
     1861 aaccagttca aggaccattt ccttggtcgg tgcaagctgg agtttacccg ggcgacaacc
     1921 tgccaaaagt gcctgcgcac gggcgatatc gagaatgtcg gccgaaccgc ctaccatcac
     1981 acattcttcg aaatgctggg caatttcagc tttggagatt atttcaaacg ggaggccatc
     2041 ctctgggcct gggaattcct aacggacaag aagtggcttg gtctcgaccc agagaagatc
     2101 tcggccactg tgtaccttga cgatgacgag gcggccaaca tttggattca ggaagtgggc
     2161 ctgtcacctg aaaaggtcac gcggatgggc gaagatgaaa acttttggcc ggccaacgcc
     2221 cccagccagg ggcccgacgg cgtttgcgga ccgtgcagcg agatttacta cgaaaccccg
     2281 tggggaaagg tcgagatttg gaatctggtg ttcacccaat tcaatcgggt gggaccaccc
     2341 cccaataatc tcaggccgct gcccagcaag aatatcgaca ccggcatggg gctagaacgt
     2401 acggcagccg tcttgcaagg ggtcgagtcg aatttacaca ttgacattat tcgtccgatc
     2461 gtcgaggcag ccgccgacgt atgccacgtc aagtatgatc cggccagcga ttttggacgg
     2521 cggttgcgac ggattgctga tcacattcgc gcctgtacgt ttgccattca tgagaacgtg
     2581 accccttcgc ccaataaaca gggttacgtg attcgccgtt tgattcggcg tgccgtgctg
     2641 gatggtcacc gcatgggtgt ccgcgagccg ttcttccaca agctcgtgcc tgtcgtggcc
     2701 gaaatgatga agaatcccta tccggaattg caggagacca ttccgcgggt caccgcagtc
     2761 atcaaagggg aggaagagaa tttctttggc gttctggaaa cgggcctgga ccgaattgaa
     2821 cgtcttttcg aggagatgga acgcagtggt cggggggtgg tctcgggggc cga
//
LOCUS       DAOPTR010000477         2870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_27861_length_2870_cov_4.378686, whole genome shotgun sequence.
ACCESSION   DAOPTR010000477 DAOPTR010000000
VERSION     DAOPTR010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27861_length_2870_cov_4.378686"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PKI05_09565"
     CDS             <1..814
                     /locus_tag="PKI05_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52494.1"
                     /translation="RVAVTGSLKFDGTEFDRNNPRTQRFRDLARIGPDEVVFMAGSTQ
                     KEEELLALQVFQELRDAFPQLRLIIVPRHPERFDEVAKMLSQSGLPWQRRSQLIDPSE
                     TIRNRAQDPDHFYREVTPQQDHSVHSSTGFPRAPVLLVDTVGELTAWWGTAHIALVGG
                     SFGSRGGQNMLEPAGYGAAVCFGPNTWNFRDIVAHLLNAKAAVVVHNFEELRQFVQRC
                     LEYPDYAATLGENARKLILAHRGATQRTADCLQKLLGQSPASATPLRLKRSA"
     gene            complement(827..1267)
                     /locus_tag="PKI05_09570"
     CDS             complement(827..1267)
                     /locus_tag="PKI05_09570"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOA52495.1"
                     /translation="MLREHEIEIRVRYPECDPMGLLHHSRVFVYFEMGRTELLRASGG
                     NYRQLEEQGLFVVVVEARCRYYRPARYDDLLRLRTRVTRMSGYKIEHEYHLFRGEELL
                     CRGQVTLAVVDRTGQVRPIPEAWQLEFSKEAGESANLRSEVQSE"
     gene            complement(1313..1939)
                     /locus_tag="PKI05_09575"
     CDS             complement(1313..1939)
                     /locus_tag="PKI05_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52496.1"
                     /translation="MAVRNLACMSLVLVAILGCSQSADHEAFRVKQSAVQPATPESKV
                     PPLASHISSEGEVTTQVGPPNLPPPERSGESFLSPPPMPQVEPGFTHSPVATPPMPSA
                     PIHQQAGTDVQVDLSAAVALPMTGPEGTMMGFSVDYRLKGVAGPPQSSYVWVIEGRPG
                     KVYRLPVKLDAQGNLMFIIQEWRPEDGPFRCRIEDYTGRIVSPVVDMR"
     gene            2164..>2870
                     /locus_tag="PKI05_09580"
     CDS             2164..>2870
                     /locus_tag="PKI05_09580"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOA52497.1"
                     /translation="MRSVRLLILMIGGCTLCLPNLYPVLGEEKPVQPLTGLVGWWPFD
                     ENGGNTAADLCPTPHPATLEGQAKFVAGRIGNAISLDGKGSRLRVPGFKGITGTSPRT
                     VSAWIKTSTASGQIVAWGSEEPGKMWIFGFVRSRIGVTPRGGYLYVKEPLHDGNWHHV
                     AVVLEEGSPPNLHDHARIFVDGELAEIHDIGLLDLWPIETGDTLDVTIGQGFNGLIDD
                     LRIYASVLTEEEIRSLF"
BASE COUNT          638 a          793 c          796 g          643 t
ORIGIN      
        1 ccgcgtcgca gtcaccggat ccttgaaatt cgatggcacg gaattcgacc gtaataaccc
       61 acggacgcaa cggttccgcg acctggctag gattggcccc gatgaagtcg tatttatggc
      121 gggtagcact caaaaagagg aggaactcct ggcacttcag gtctttcagg agttgcgtga
      181 cgcttttccc cagttgcggc tgattatcgt gccaaggcat cctgaacgtt ttgatgaagt
      241 tgctaagatg cttagccaaa gcggcctacc ctggcaacgt cggtcacaac tgatagaccc
      301 gagcgagact atccgaaatc gcgcacaaga cccagaccat ttttaccgcg aagtcacgcc
      361 acagcaggat cattcagtcc actcgtcaac cggtttcccc cgggcaccgg ttcttcttgt
      421 cgatacggtg ggagagctta cagcctggtg gggaacggcc cacatcgccc tggttggtgg
      481 aagcttcggc tctcgtggtg ggcaaaacat gctggaaccc gcaggttatg gcgccgctgt
      541 ttgctttggt cctaacacgt ggaatttccg cgacattgtg gcccacttgc tcaacgccaa
      601 ggcggccgtg gtcgtccaca actttgagga actccgccaa tttgtccaga gatgcttgga
      661 atatccggac tacgccgcca cactcgggga gaatgcgagg aagttaatcc ttgcgcaccg
      721 cggtgccact caaagaaccg ccgattgcct tcaaaagctt cttggccaga gcccggcttc
      781 agccacccct ttgcgattga aacgatcagc ttgacgggtc aataaattat tcgctttgaa
      841 cctctgaacg gagattggca gactctccag cctctttcga gaattcgagt tgccaggcct
      901 cgggaatggg ccggacctgg cccgttcgat caacgacggc caacgtcact tggcctcggc
      961 agaggagttc ctcaccgcgg aaaagatggt actcgtgctc gatcttatag ccgctcatgc
     1021 gagtcacgcg tgttctcaat cgcaacagat cgtcataacg tgccggacga tagtagcgac
     1081 atcgtgcctc gactacaacc acaaatagtc cctgttcttc taactggcgg taattgccgc
     1141 ctgatgcccg tagtaactcc gttcggccca tctcgaaata gacaaagacc cggctgtgat
     1201 gcaacagtcc catcgggtcg cattcgggat agcgaacacg aatttcaatc tcgtgttctc
     1261 gtagcatgaa atcctcctgc cattcctgtg gggccaaccc aagatgccgg gcctaccgca
     1321 tgtcaaccac cggcgaaaca atccgacccg tgtaatcctc gatccggcaa cggaatggtc
     1381 cgtcttccgg tcgccactct tggatgataa acatgaggtt gccttgtgca tccagtttca
     1441 ccggtaatcg atagaccttt cccggcctac cttcgatgac ccaaacgtag gaactttgcg
     1501 gagggcccgc tacgcctttc agacggtaat ccacactgaa gcccatcatt gtcccctccg
     1561 gcccagtcat aggaagggcc acggccgctg agagatccac ctggacgtcg gttccagctt
     1621 gttggtgaat tggcgccgag ggcatcggtg gcgtcgcaac cggcgaatgt gtgaaaccag
     1681 gctcgacctg cggcatcgga ggaggcgaga gaaagctttc accggatcgc tccggaggag
     1741 gcaggttcgg cggccccacc tgggtcgtta cctctccctc ggacgaaatg tgactcgcca
     1801 agggcggtac ctttgattcg ggtgtcgccg gttgaacggc cgattgtttt acacgaaagg
     1861 cttcatgatc tgccgattgc gaacagccca gaattgccac tagcacgagc gacatacagg
     1921 ctagattgcg taccgccatg actgcgtctc cttgtggcca tgtcggcatg ccaggacacg
     1981 ccaacaaacc cagcttcgcc gaagcaattg cgtcttcaga tcagtgtaaa cctagaatca
     2041 tttgcccgcc aagaggctct ttgggtgcgc aacgtcgctt gcaatccgat gtcgcagggg
     2101 ccaaaatgga caacgtgccg gcagacttga gaaaacacaa tcggagtcac tggggaggaa
     2161 catatgcgaa gtgtgcgact attgattttg atgattgggg gatgcacact ttgtttaccg
     2221 aatttgtatc ccgtcttggg agaggagaaa cccgtccaac cgctgactgg gttggtcggc
     2281 tggtggcctt tcgatgaaaa cggaggcaat acagcggccg atctttgtcc cacgccacac
     2341 cccgccactc tggagggcca ggccaaattt gtcgcaggtc gcattggtaa tgctatatcc
     2401 cttgatggga aaggaagccg tctccgtgtt ccgggtttca agggaatcac aggaacctcc
     2461 ccacgaacgg tctctgcgtg gatcaagacg agtacggctt cgggacaaat cgtcgcctgg
     2521 gggagtgaag aacctggcaa gatgtggatt ttcgggtttg tccgatcccg gattggtgtt
     2581 acgccgcggg gtggctatct gtatgtgaag gagcctctcc acgacgggaa ttggcaccat
     2641 gtggctgtgg ttttggaaga agggtcaccg cctaatcttc atgaccacgc cagaatcttc
     2701 gtagatggtg agttggctga gattcatgac atcggtttgc tggatctgtg gcctattgag
     2761 acgggcgaca ccttggatgt aaccatcggt caagggttta atggcctgat tgatgatctt
     2821 cgcatttatg ccagtgtatt gaccgaagaa gagatacgca gcctgtttaa
//
LOCUS       DAOPTR010000478         2868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_27888_length_2868_cov_3.185567, whole genome shotgun sequence.
ACCESSION   DAOPTR010000478 DAOPTR010000000
VERSION     DAOPTR010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27888_length_2868_cov_3.185567"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1935
                     /locus_tag="PKI05_09585"
     CDS             <1..1935
                     /locus_tag="PKI05_09585"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOA52498.1"
                     /translation="FAGFGGIRYPVAVTFSLVTGVCWAVGFVVIAVGHLNILTSAFGA
                     MLIGLGDYGVHFVAHYLDRKKRQPNVRDTLIETAETVGPGIATGAVTTAVAFFAGAFT
                     DFLGVAELGIIAGGGILLCWLADMTILPAFLCLIDRDGLRSQKSALLDLTPWFRPIAH
                     FPKAVTLGVGAVVTLLAMGLPHLRYDYNLLHLQPKGLESVEYQNKLVEHMSRSSYFAL
                     SIAANPEEAKRRKAEFLKLPTVDHVEDLVSVLPDDVAVKRTLITQLAAELKDWSCLDT
                     NIPSREVLDQGLAGLQMALQHQPHAESAQLQMIVNHLRTTIRSLPAEEYTRRLSYLTQ
                     TIQREILPAFASLALLTQQLDPPQLDDLPQPLVERFVGKNGRHLLQVYCKGDFWDPVT
                     MRQFVADIRSVDPAATGNPIQIYEACHQMNRAYIEAAIYAFVAILLTLYLDFRSIKDV
                     LLSLLPLILGMISLFGLMGWFDLPLNPANMITLPLILGIGIDDGVHILHDFRRQGNRY
                     RMPEGATLTAVTINSLTTLVGFGALMASPHCGLESLGRVLTFGMAFCLVTALLMPGFL
                     RWASGLLRGNRDSTEWETELQHILKGVTAGQHPTSEPHQAATNPPRVVAPLSAAEEQP
                     QSEQSQRAAISVKIRPRRAA"
     gene            complement(1965..2300)
                     /locus_tag="PKI05_09590"
     CDS             complement(1965..2300)
                     /locus_tag="PKI05_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52499.1"
                     /translation="MMRLAILGIITGVMIFCVVGLATVLTLAAVVSGMGDVTAGIVLR
                     YVALGIGVFLAVDTVVLLLWLVFLTVTAELALNQVIAAKKDQDKQMDSTTVAMENLAD
                     SEWEETSSG"
     gene            2550..>2868
                     /locus_tag="PKI05_09595"
     CDS             2550..>2868
                     /locus_tag="PKI05_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52500.1"
                     /translation="MSLTRATQSRCLLLTTSLALAVWIGVLSDCQAEEVVLLDGRVLK
                     GKLGIVTGLVDVPRMVDPESGGPIPLIVFVDDDLRRTFISKRRIREIRPGSGPEIVER
                     FRLR"
BASE COUNT          615 a          824 c          709 g          720 t
ORIGIN      
        1 tttgcaggct tcggtggaat tcgctatcct gttgctgtca cattttcctt ggtgaccggt
       61 gtgtgttggg ccgtgggatt tgtcgtaatc gccgtgggac atctcaatat cctaacgagt
      121 gccttcgggg cgatgctcat cggccttggt gattacggcg tccattttgt tgctcattac
      181 ctcgacagaa aaaaacggca accgaacgtc cgagatacct tgatcgaaac ggcggaaacc
      241 gttgggccag gcatcgccac aggcgccgtg accacggcag tcgctttttt tgcgggtgcc
      301 ttcaccgatt tcttgggtgt cgccgagcta gggattatcg cgggtggagg aatcctcctt
      361 tgctggctag ccgatatgac gatacttccg gcctttctct gtctgatcga tcgcgacgga
      421 ctgcgttccc aaaagtctgc ccttcttgat ctaacaccgt ggtttcgccc tatcgcccac
      481 ttccccaagg cggtcacctt gggtgttgga gcggtcgtca ccctattggc aatggggtta
      541 cctcatctcc gttatgacta caacctctta cacctccagc ccaagggact cgaaagcgtg
      601 gagtaccaaa acaagctcgt cgagcatatg agccgaagta gttattttgc cctttctatt
      661 gctgctaacc ccgaagaggc caaacgccgc aaggccgagt ttctcaagct ccccaccgtt
      721 gatcacgtgg aagatcttgt ttccgtcctt cccgatgatg tcgcggtgaa acgcacactc
      781 atcacacaac tggctgctga actcaaggac tggtcctgtt tggacaccaa catcccttcg
      841 cgagaagtcc tcgatcaagg cctagcgggg ttgcaaatgg ccttgcaaca tcaaccccat
      901 gcggagagtg cccagcttca gatgatagtc aatcaccttc gcaccaccat ccggagtttg
      961 ccggcggagg aatacacacg ccgcctttcg tatttgacgc agacaattca gagggaaata
     1021 cttcccgctt tcgccagcct cgctcttctc acccaacagt tggatccacc ccaacttgat
     1081 gacctgcctc aaccgcttgt ggaacgtttt gtcggaaaaa atggccgaca tcttctgcaa
     1141 gtgtactgta agggtgattt ttgggatcca gtgacgatgc gacagttcgt cgcggacata
     1201 cgcagcgttg atcccgcggc aacgggaaat cctatccaga tttatgaggc ctgccatcag
     1261 atgaatcgag cctacatcga agctgcgatt tacgctttcg tcgccatcct tcttaccttg
     1321 tatcttgatt ttcgcagcat caaggatgtt ctcctgtcct tgcttcctct tatcttggga
     1381 atgatcagcc tttttggact aatgggttgg ttcgacctgc cacttaaccc cgccaatatg
     1441 attacattgc cccttatact agggatcggc atcgacgatg gggtgcacat cctgcacgat
     1501 tttcgccgcc aaggaaatcg ctatcgcatg ccggaagggg ccacgctgac cgcagtgacg
     1561 atcaacagtc taaccaccct cgttggtttt ggggccctca tggcctcgcc tcattgtgga
     1621 ctggaaagcc ttgggcgggt cctgacgttt ggcatggcct tttgccttgt cactgcccta
     1681 cttatgcccg gatttctcag atgggcatcc ggcttgcttc gaggaaatcg cgattccacc
     1741 gaatgggaaa cagagctgca acacatcctc aaaggagtta cggccggaca acatccaact
     1801 tccgagccac atcaagcggc tactaaccca ccacgagtag tggctccatt atctgctgct
     1861 gaggagcaac cccaatccga gcagtcccaa cgcgccgcga tctctgttaa aatccgtcca
     1921 agacgggcag cttaagacgc ggagtcattc ggtgattcca atcatcagcc ggacgaggtc
     1981 tcttcccatt ccgagtctgc caaattctcc attgcgacgg tcgtcgaatc catttgttta
     2041 tcctggtctt ttttcgcggc gattacctga tttaaagcca attctgccgt gaccgtcaaa
     2101 aacaccagcc acaagagaag aaccaccgta tccaccgcca ggaagacccc gattcccaaa
     2161 gccacgtatc gtagcactat gccggctgtg acgtcaccca ttcccgatac aacagcggcc
     2221 agtgttagga ccgtcgccaa tcctacaacg cagaaaatca tcaccccggt gataatccct
     2281 aaaattgcta atctcatcat gatctccgca aaaacgagtg cacaacccaa caagatggtt
     2341 tgatgcaggg caggccaatc tggccccaat ccttgtcttc ccggtgaatc atgacttatt
     2401 ttctcatttt gccgcaatca tgcaaatcgg ccctgtttga taattttgcc gcgccgccgc
     2461 acgacttgtt ttggcggcct ccgtctttcg agataataag attgaggttg aagtaggttc
     2521 tcaaatcgcc ttattggatt agaactggca tgtccttgac ccgcgctacc caatctcgct
     2581 gcctcttgct gacaacttcc ctggcgctcg ccgtctggat cggtgttttg tcggattgcc
     2641 aggccgagga agtggtgctt ctcgatggcc gtgtcttgaa aggcaagctg gggatcgtga
     2701 cggggctcgt ggatgttccc cgcatggtgg accctgagag tggagggccg atcccgctga
     2761 ttgtctttgt cgacgatgac ctccgccgaa cgtttatctc taagaggcgg attcgcgaga
     2821 ttcggccagg cagtgggcct gaaattgttg agcgttttcg attacgtc
//
LOCUS       DAOPTR010000479         2864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_27928_length_2864_cov_3.593094, whole genome shotgun sequence.
ACCESSION   DAOPTR010000479 DAOPTR010000000
VERSION     DAOPTR010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27928_length_2864_cov_3.593094"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PKI05_09600"
     CDS             complement(<1..482)
                     /locus_tag="PKI05_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HOA52501.1"
                     /translation="MEQHDRRSIICVTIARGRHRHVIAEHRHLAEHGAKLVEIRLDYI
                     NGPVNVKRLLAERPCPVIMTCRRERDGGRFNGAEQQRQLILRTAIAEGVDYIDLEEDI
                     AAEIPRFGQTKRIISYHNFRETPADLQALHERMSQLDPDIIKIATLANHPQDNLRMFR
                     L"
     gene            complement(773..2050)
                     /gene="rho"
                     /locus_tag="PKI05_09605"
     CDS             complement(773..2050)
                     /gene="rho"
                     /locus_tag="PKI05_09605"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOA52502.1"
                     /translation="MGRKRRNGAMRSKNGQNGSAAEVNASRNGGSASQDTKTTVTAVE
                     TLDSTELHEGGGVLELHPSGYGFLRDPAANYERRMSDPFVPGSMIEKYGLRQGVYIRG
                     LVQPGKKGQGPRLREIHDVDGLPPQEYVNVKNFDELTPINPEEWLRLETGPEPLTTRV
                     MDLLTPIGKGQRALIVAPPRTGKTVLLQHISQAIATNYPEMKLIILLIDERPEEVTDF
                     RRVCKNAEVVASSLDCEIESHVRLSQLIVERCRRLAEMGKDVFLLMDSITRLARAFNK
                     WVGNTGRTMTGGIDVKAMDVPKKLFATARAFEEGGSLTIVATALIDTGSRMDELIFQE
                     FKGTGNMELVLDRRLADRRIWPAIDITQSGTRREEKLLPPDVLRAVTILRRMLSSMNP
                     VDAMEQLTAKLAKYKSNREFIELITGAHANVSD"
BASE COUNT          593 a          874 c          737 g          660 t
ORIGIN      
        1 aggcgaaaca tccgcagatt gtcctgagga tgattagcca gcgtggcaat cttgatgatg
       61 tcggggtcga gctggctcat ccgctcatga agggcctgca agtccgccgg ggtctcccga
      121 aagttgtggt agctgataat ccgtttcgtt tggccaaacc gtgggatctc cgccgcgatg
      181 tcctcttcca ggtcgatgta atcgaccccc tcggcgatgg ccgtccgcag gatgagttgc
      241 cgctgctgct cggccccgtt gaaccgaccg ccatctcgct cccgacgaca cgtcatgatg
      301 acggggcacg gcctttcggc gagaagccgc ttgacgttga ccgggccgtt aatgtaatcc
      361 agccgaatct caaccagttt cgccccatgt tccgccaaat gacgatgctc cgctatgacg
      421 tggcgatgac ggccccgtgc gatggtgaca caaatgatac tccggcgatc atgttgttcc
      481 ataataggcc ctcataggat cagacgaaca caacgtggac cgcgttggct tcgatacgtc
      541 gcttctcccc gtgcgtgcta cgacgcttgt gcctttccag actctcagat ggccatgctc
      601 ttttctcgcc catcgaccgg cgaacggctc atctcattta tggtacactt ggcgcggacc
      661 gttcaccacc tgactcggga gaaggatgcc tcaccaggaa gccgtcatga tcgtatttgc
      721 gaacgagaga tgcacgctag actcgatgcc aggtcgagga ctttccctca catcaatcac
      781 tgacgttggc gtgcgcaccc gtgatcagct cgatgaattc ccgattggac ttgtacttgg
      841 ccaacttggc cgtcaactgt tccatggcat ccacgggatt catgctggac agcatccggc
      901 ggaggatcgt aacggcccgc agcacatccg gcggcaagag cttttcctca cgccgcgtgc
      961 ccgattgggt aatatcaatc gccggccaga tacggcggtc cgccaaccgg cgatcaagga
     1021 ccagctccat gttgcccgtg cctttgaatt cctgaaagat cagttcgtcc atgcggctac
     1081 ccgtgtcgat caaggccgtg gcgacaatgg tcagtgagcc gccttcctcg aaagcccggg
     1141 ccgtggcgaa caacttcttg ggcacatcca tggccttgac gtcaatacca ccggtcatcg
     1201 tccgaccggt gtttcctacc catttgttga acgctcgggc caaccgcgtg atcgaatcca
     1261 tgagcaggaa gacatccttc cccatttctg ccaaacggcg gcaccgctcc acaattaact
     1321 gagacagtcg cacgtggctt tcgatttcgc aatccaggct gctggccaca acttccgcgt
     1381 ttttgcacac ccgacggaaa tcggtcacct cctcggggcg ttcgtcgatc agcaagatga
     1441 tcaacttcat ttcgggataa ttcgtggcga tggcctgact gatgtgctgc aagagcaccg
     1501 tcttgccggt tcgcggcggg gccacgatca aggccctttg ccccttacca attggcgtga
     1561 gaaggtccat cacgcgggtg gtgagcggtt ccggtcccgt ctccagtcgc agccactcct
     1621 cgggattgat gggcgttaac tcatcgaagt tcttgacgtt gacatattcc tgcgggggta
     1681 ggccatccac gtcgtgaatc tcgcggagtc gtggtccctg tcccttcttg cccggctgga
     1741 ccagacctcg aatatagacc ccttgccgga gcccatactt ctcaatcatg ctacccggca
     1801 cgaagggatc actcatccgc cgctcgtagt tggctgccgg atcacgaagg aacccgtagc
     1861 cactgggatg gagttccagc acgccaccac cctcgtggag ttccgtgctg tcaagcgttt
     1921 ctaccgcagt gaccgtagtc ttggtgtctt gcgatgccga accgccgttg cgagaagcat
     1981 ttacctcagc ggcagagcca ttttgtccgt tcttcgaccg catagcaccg ttacgccgtt
     2041 ttcgacccat tgaatcaccg aaagaaaaag gatttttctc ccctacaaag cttgttaaaa
     2101 gagcaactga cgcatgtctg ccaagaaaat cgttttcctt ttagcgcagc ctcatgccga
     2161 cgaaatcatc aagaaaggcc cccactaccg tcggcgaaag gctctcttaa aaggaatgag
     2221 cacgcaacgc cattgacggt ctcgtcagac caagcgcttg atagctgctt tcaccaaatc
     2281 caataacgat tcttgtaagc ccaccggtag ccgcgttgtg cgtttcgtcg ttcgcagcga
     2341 tgtgttgatc gaccggaagt cgcccagcga aatgaacgaa tcaacaccga gctgtctttg
     2401 tcgagccgcc tcgatcgctc aagttcttga gaaacgccta cggcgcttca gccgtgtttc
     2461 tcacaaagag agagggaaaa atttcagaaa cccctgattt cccaatggct acccgacgct
     2521 tagagcctta ccactacaat catatcgtaa ggccccaccg tgtcaagaca gcttccgccc
     2581 tttacacaag tttaacaaaa cttttctttg gtgcgctttc tcggcgactg aatcgtttgt
     2641 gacggctgtc ccgattgcgc cagccatccc ccagccccga tatccgagga cggacgggcg
     2701 caacctagtt ccggctacct tcattatcga ctcaacggtt tcgctagatt tatttttcgt
     2761 cggccgctgc cctgccgcgg aaccaactag ttcctctcat tgttcacccg gtggatagag
     2821 aatcttttgt tgctcctccg gtgtgaaacg gctgttggcg accg
//
LOCUS       DAOPTR010000480         2864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_27930_length_2864_cov_3.446778, whole genome shotgun sequence.
ACCESSION   DAOPTR010000480 DAOPTR010000000
VERSION     DAOPTR010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27930_length_2864_cov_3.446778"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            444..983
                     /gene="ilvN"
                     /locus_tag="PKI05_09610"
     CDS             444..983
                     /gene="ilvN"
                     /locus_tag="PKI05_09610"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOA52503.1"
                     /translation="MRHILTALVQNVPGVLSHIAGMLASRGYNIDSLAVGETEDPRLS
                     RMTFVVVGDDAVIEQVRKQLEKIVTVVRVDDVSSRDYVERDLMLIKVAAPHEKRGEIR
                     ELVEIFRGRIVDVAPQSMIIEISGQEKKVESFIELMRPFGILELVRTGRIAMVRGETT
                     LKFDENNNGQEYLVSHLAR"
     gene            1031..2035
                     /gene="ilvC"
                     /locus_tag="PKI05_09615"
     CDS             1031..2035
                     /gene="ilvC"
                     /locus_tag="PKI05_09615"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007339869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOA52504.1"
                     /translation="MPAKIYYDEDADLSLLKGKTVAIIGYGSQGHAHAQNLRDSGVRV
                     IVAELPGTANYKLAVEHGFQPMSAEEASKQADIITILLPDEIQPDVFRSSIRPNLRAG
                     NVIVCSHGFNVHYGQFDIPDGVAAVLVAPKGPGHLVRSEFVKGGGVPCLVANGPNGTE
                     ETFKLALAYAKGIGGTRGGVIETTFAEETETDLFGEQVVLCGGVSELICAAFETLVEA
                     GYQPELAYFECMHELKLIVDLMYQGGLSYMRYSISNTAEYGDYTRGPRIINQQTRAEM
                     KKILAEIQSGAFAKEWILENKANAPMFKALRKKARAHMIEEVGRKLRKMMKWIDAKEV
                     "
     gene            2267..>2864
                     /locus_tag="PKI05_09620"
     CDS             2267..>2864
                     /locus_tag="PKI05_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HOA52505.1"
                     /translation="MSDSSADFSRFHCPTPLATHETIQLAHGGGGRLMRQLINEVFLP
                     AFRREGDTEGLRQPPHDSAEIRLGDGTRMAFTTDAFVVSPLFFPGGDIGKLAVFGTVN
                     DLAMSGAKPLWLSASFIIEEGLPMEILSQIVRSMREAADEASVRIIAGDTKVVDRNKG
                     DGLYISTTGLGWIPEDVHISPAHIRPGDKVILSGDIGRH"
BASE COUNT          667 a          725 c          832 g          640 t
ORIGIN      
        1 atcaaacaac cgaataacct ccggcgtgtc ctggacgctg gcttcctctg cgacggccat
       61 taaccctaca caacagagga ccgataacaa gccaatcacc cccagccggc agaccaacgg
      121 ccttgcgtaa cttgccttaa agagccatct tgccatgtgc taagtccttt tcttgaaacg
      181 ggcagatttc ggtatgtcgt aaataaagca tttcgtatta tgatgccaaa gttcggaggc
      241 aaaatcaaaa tgatgcccta aaacccggcc gaccgaacgg taacacgcga tccaagcgaa
      301 ccttacgctc agatgacatg tccgacttga gggggactct ctgaaaccgg cagacgggct
      361 ccctgacgcc ggacggcttg tcattcccct taaggagcgt taggatagtc attttacgca
      421 gaaagcagga gtcatcagcg gttatgagac acatcctgac agcacttgtg cagaacgtgc
      481 ctggggtact ctcgcatatt gcggggatgc tcgcctcgcg cggatataac attgacagct
      541 tagccgtcgg tgaaaccgaa gacccgcggc tttctcggat gacattcgtg gttgtgggtg
      601 acgatgccgt tatcgagcag gtacgaaagc aactggaaaa gatcgtcacg gtcgttcgcg
      661 tggacgacgt aagcagccgc gattacgtcg agcgagactt gatgctgatt aaggttgcgg
      721 ccccgcacga aaagcgaggc gaaatccgcg agctcgtgga gatcttccgg ggacgcatcg
      781 tggatgtggc tccgcaaagc atgattatcg aaatatcggg ccaggaaaag aaggtggaat
      841 ccttcatcga gttgatgcgc cccttcggca ttctagaact tgttcggacc ggacgaatcg
      901 ccatggtaag gggcgaaacg acgttaaagt tcgacgaaaa taacaacggt caagagtact
      961 tggtgagtca cctcgcgcgc taagcgctaa gagtcgggat aacagtccaa gataaacagg
     1021 agtttccgtc atgccggcga agatttatta cgatgaagat gccgatttgt cgcttttaaa
     1081 agggaaaacc gtggccatca tcgggtatgg ctcccaggga catgcccacg cccagaattt
     1141 gcgtgacagc ggtgttcgtg tcattgtggc cgagctgccc gggacggcca actacaagct
     1201 cgccgttgag cacgggttcc agcccatgtc ggcagaggag gcaagcaagc aggccgatat
     1261 catcaccatt ttgctgccgg atgaaattca gcccgatgtg ttccgcagtt caatccgacc
     1321 gaacctacgg gcggggaatg tgattgtgtg ttcccacggc ttcaatgttc attacgggca
     1381 gttcgatatt ccagatggcg tggcggccgt cctcgtggca cccaaggggc cggggcatct
     1441 cgtgcgatca gaattcgtca aaggcggtgg tgtcccctgt ttggtcgcca atggtccgaa
     1501 cggcaccgag gagacattca agctcgcttt ggcttatgcc aaaggtatcg gcgggacccg
     1561 cggcggcgtg atcgagacca cctttgccga ggaaaccgag actgatttgt tcggcgagca
     1621 agtcgtcctc tgcggtggcg ttagcgaatt gatttgcgcc gcttttgaaa cgcttgtgga
     1681 ggctggttat cagccggagt tggcctattt cgaatgcatg cacgagttga agctaattgt
     1741 ggatctaatg tatcaaggtg gtctgagcta catgcggtat agtatctcca ataccgcaga
     1801 gtacggtgac tacacgcgag gaccgcgtat tatcaatcag caaacgcggg cggaaatgaa
     1861 aaagattctg gccgagattc aatccggggc ctttgccaag gagtggattc tggaaaacaa
     1921 ggcgaacgcc cccatgttta aagctctacg gaaaaaggcc cgcgcccata tgattgagga
     1981 ggttggccgg aagctgcgaa aaatgatgaa gtggatcgac gccaaagagg tttgatctcc
     2041 accgtttgat tcattggtct gtcgctcgcc caaacttgct ccctagcagg tttgggtttt
     2101 ttctttcttg tggaccgagg gccatgccac ggtcgaggac atagcggttt ggccaccgat
     2161 tcgcctataa tgttgtggtg cctgtggatg aaagcctggt cttcaggaaa ttgcttctta
     2221 gatgctttat ttgcgaagaa cacttcaaat acgatagggt tgatctatgt cagattcgtc
     2281 agccgatttc agccgttttc attgtcctac gcctcttgcc acgcatgaga cgatccaact
     2341 ggcccacggc ggtggtgggc gactaatgcg gcaactcatc aacgaggtgt tcttgcccgc
     2401 ttttcgccgt gagggtgaca cggaggggct gcggcaaccg cctcatgaca gtgccgaaat
     2461 ccgtctgggg gatggcactc gcatggcctt cacaacggat gccttcgtgg tgagccccct
     2521 gttctttccc gggggtgaca ttggcaaact ggcggtgttt ggaaccgtca acgacctggc
     2581 aatgtcgggg gcgaaaccat tatggttgag tgccagcttt atcatcgagg aaggattgcc
     2641 catggagatc ctctcgcaga tcgtcaggtc catgcgcgag gccgccgatg aagcgagcgt
     2701 ccggatcatt gccggggata cgaaggttgt cgatcggaat aagggggacg gactttatat
     2761 cagcacaacc ggcctaggat ggattccgga ggatgttcac atttcgccgg cacatattcg
     2821 accgggggac aaggtcatcc tctccggcga tattgggcgc cacg
//
LOCUS       DAOPTR010000481         2858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_27996_length_2858_cov_2.445951, whole genome shotgun sequence.
ACCESSION   DAOPTR010000481 DAOPTR010000000
VERSION     DAOPTR010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27996_length_2858_cov_2.445951"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PKI05_09625"
     CDS             complement(<1..231)
                     /locus_tag="PKI05_09625"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012500.2"
                     /note="catalyzes the decarboxylation of diaminopimelate
                     into lysine; pyridoxal phosphate(PLP)-binding; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOA52506.1"
                     /translation="MAEAGYLVTEIRAVKKQADNLFYLVDAGFNNLARPVMYGAYHPI
                     SVVPRDRNFARPEYDVIVGGPLCESGDIFTQEE"
     gene            complement(238..1047)
                     /locus_tag="PKI05_09630"
                     /pseudo
     CDS             complement(238..1047)
                     /locus_tag="PKI05_09630"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="catalyzes the decarboxylation of diaminopimelate
                     into lysine; pyridoxal phosphate(PLP)-binding; internal
                     stop; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
     gene            complement(1103..1903)
                     /locus_tag="PKI05_09635"
     CDS             complement(1103..1903)
                     /locus_tag="PKI05_09635"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOA52507.1"
                     /translation="MERMLLVAVDIGNSRIKCGIFYPEGWRRKSPEVNCVFSAGLYDP
                     AWRDFADPLFAAVAEVPRVTWWIASVNRPVTTDFLEILRAARPKDEVMLVSSSDLPLE
                     VAVPHPDRVGIDRLAAAVGANTLRDPNRPAIVVGVGTAVTVNAISKEGVFLGGAISCG
                     AAMSARALHEFTDLLPLIPTEWQTAPESIGKETPSALAAGIFWGLVGTVRELIERQTE
                     VLQGPPEVFLTGGGAKPLVGHLGDDVRFVENLTLIGIAAAARAVLDKG"
     gene            complement(2165..2858)
                     /gene="cgtA"
                     /locus_tag="PKI05_09640"
                     /pseudo
     CDS             complement(2165..2858)
                     /gene="cgtA"
                     /locus_tag="PKI05_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910836.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
BASE COUNT          642 a          871 c          724 g          621 t
ORIGIN      
        1 ttcctcttgc gtaaagatgt cgcccgactc gcacaagggg ccaccgacga ttacgtcgta
       61 ctctggcctt gcgaaattcc gatcacgtgg gacgacgcta atggggtggt aagccccgta
      121 catcacaggg cgggcaaggt tgttaaaacc ggcatcaacc agataaaaga gattatcggc
      181 ctgtttcttc accgccctaa tctctgtcac taagtagccc gcttcggcca caagataccg
      241 cccaggttca atctccaatc gtacaaggtg tccgaatgcc gactccaact tcctgcgagt
      301 tgtgttccaa agctcaaaat agcgttccag atcaatccgc tggtccgaat cacgataggg
      361 gattggaagc ccgccgcctg cgctgatggt ccgcacactt ggtcccacgg caaaagcgac
      421 ttcttccatc gcgctacaca ccttggccag gtgctcaaag tcacttcccg atcagatgtg
      481 catgtgaagc cccgtgacgc tcaggccaag ttctttggcc tgtgccagtg cctcgccaag
      541 ttctttgaac catatcccgt gtttggaaaa ctcaccaccg gtgttggtct ttcggctatg
      601 accgtgccca aaaccgggat tgatccgcaa agtgatctcg cgccctggcg cccgctcgcc
      661 caactgctgg atcatgtcgc gggaaccaca gttcacgtgg attccttttt ccacaaccag
      721 ctcgagagaa tcccgatcga aaatatcggc ggtatagaca attggcgggg gatcacccac
      781 ggctgcgtaa ccggcgacta acgcacgatg aatttccccg gcactaacag catcaaccag
      841 tgcccccaat tgccgcagca aggacaggat ggcgagattt gaacaggcct tttgggcgta
      901 gcggacaaca tcaaacttgc gaagttcgtt gaaccgaaga cgaatcgttt ccgcatcata
      961 cacgtagagc ggtgtcccga attgctcggc aaggctaacc acatcgattc cggcaatgtc
     1021 gcaacgtagt gtttttcttt cagccatatg tgttaccttt tccatgatgt cgtatgataa
     1081 cttcgctgcc acatgtgact tctcatcctt tgtctaacac cgcccgggcc gctgccgcaa
     1141 taccaataag tgtcaagttc tcaacgaaac ggacatcgtc acccaggtgt ccgaccaagg
     1201 gtttggctcc ccctcctgtc aaaaaaacct caggaggacc ctgaaggacc tcggtctggc
     1261 gttcgataag ctcccgcact gtgcccacca atccccagaa gataccggcg gccaaggccg
     1321 agggcgtctc tttcccgatg ctctcaggcg ctgtttgcca ttctgttgga atgagtggca
     1381 ataaatcggt gaattcatgc aaggccctgg cagacatcgc agcaccgcag gaaatggctc
     1441 cgcccagaaa gacgccctcc ttcgaaatag cattgaccgt aacggcggtc cctaccccga
     1501 ccacaatggc cggacgattt gggtctcgca gtgtattcgc cccaaccgcc gcggccagcc
     1561 gatcaatccc tacccgatca ggatggggaa cggcaacctc caggggcaaa tctgatgacg
     1621 acaccaacat gacttcgtcc ttgggcctag ccgctcgaag gatttccaga aaatccgtcg
     1681 ttaccgggcg attgacactg gcaatccacc atgtaacgcg cggcacttcc gcaacggcgg
     1741 cgaacagagg atctgcgaaa tcgcgccacg ctggatcgta aagcccagct gaaaagacac
     1801 agttgacctc gggagacttc cttctccagc cttcgggata gaatatccca cacttgattc
     1861 ggctgtttcc aatgtcgact gcgaccaaca acatccgttc catgaggccg taccatgcga
     1921 tgaatcctgg caaatcgtcc gagataatcc tcccattatg gagattcttc gcagttcact
     1981 gcaatgtccg tggataagtt gctaccagcc tcgccggcgt cctttctttg cccacaactg
     2041 taacagctaa atttgcttac aaagagcctc tggcgcaagc ttgagatggg cgtgcaagtg
     2101 agactcctcg gcggaggcga ggtgcaagaa gttagcgttc gctcccacgc cgtgacgctc
     2161 gagactcttg cagcgtcttc caaatcgtct ccaccagtac gtgaaggttc tcgcccgtca
     2221 ctgcggaaac tgtgaggata ggtttgttga tcttctcctc aagcttttgg gctaccgagc
     2281 ggacactcgg caagtcggcc ttcgtgacga ccacaatttc gggacgctga accagtgctg
     2341 gatcgaactt ttccatctcg tctcgaatgc tgaaatagtt cgaaatgggg tccgagccgt
     2401 cgtaaggttc cggctcaatg agatgcagta ggataactgc ccgctggatg tggcggggga
     2461 actcgtggcc caaacctgcg ccgcgatgcg caccttcaat cagtcccgga atatcggcca
     2521 acacgaaact gcgctcgccg tcaaccacaa cccgaccgag atggggaaac ctcgtggtga
     2581 acgggtaggc tgcaatcttc ggacgtgccc gactcaaccg gctcaggagt gtgctcttgc
     2641 cggcattagg tttacccacc aatcccacat cagcgatcag ccgcaattcg agacgcaacc
     2701 gtcgcttttc gccttcggta ccgggtgtgc actcccgcgg tgcccgattg gttggcgact
     2761 tgaacgccgc attccccttt ccccctttcc cgccccttgc caccacgacc ttttcgcctg
     2821 gtcgctggag gtccttaagg aggaggttat gctcggca
//
LOCUS       DAOPTR010000482         2855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_28017_length_2855_cov_3.897500, whole genome shotgun sequence.
ACCESSION   DAOPTR010000482 DAOPTR010000000
VERSION     DAOPTR010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28017_length_2855_cov_3.897500"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PKI05_09645"
     CDS             <1..573
                     /locus_tag="PKI05_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52508.1"
                     /translation="EGVVAKYVFNQIAPFMLTSETGIMPLWRGRILHLRGNLVGEEGA
                     AHYYQLARPPERLIDSGQLAADEQLMYTIAKLNASYWLGLIAVDQGNRKSAEDYLINR
                     TAMVLPDQPWHSGVLYALGRLNEKLGDYSQAITTYRSEFQLPNRFGNLVRARLIEELT
                     GAKPLEISPEPTTNKADKASQPDKTSGKQE"
     gene            952..1176
                     /gene="rpmE"
                     /locus_tag="PKI05_09650"
     CDS             952..1176
                     /gene="rpmE"
                     /locus_tag="PKI05_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOA52509.1"
                     /translation="MKEGIHPKYVECKVTCGCGNSFVTRSIKPELKIDICNVCHPFFT
                     GKLKYVDTTGRIEKFKSKFAAGYSSVGKKK"
     gene            1326..2399
                     /gene="prfA"
                     /locus_tag="PKI05_09655"
     CDS             1326..2399
                     /gene="prfA"
                     /locus_tag="PKI05_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOA52510.1"
                     /translation="MRKELDEKLARFEELERLLHDPAVLSDPSRMATIMREHGSLAKV
                     AVKYRKFKEINQQILELRQWIEGEDPELRDLAEEELPRLRQEREALWDELLELALGGE
                     DANRTRCIMEIRAGTGGEEAALFARDLFEMYRRYAESKGWKLEILGMNPTELGGFKEI
                     SFGLEGEEVFRRLQYESGGHRVQRVPETEAKGRIHTSLATVAVLPEPEDIEIDIKPED
                     YRRDTYHASGPGGQHVNKTSSAIRLTHYATGIVVQCQDEKSQHKNFAKALRVLKTRVY
                     EYYRRAKEAERAQERRSKIGSGDRSERIRTYNFPENRVTDHRINLTLYQLDQIMEGNL
                     DPLIEALLDYERNELRLTFGSLD"
     gene            2667..>2855
                     /locus_tag="PKI05_09660"
     CDS             2667..>2855
                     /locus_tag="PKI05_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008700192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOA52511.1"
                     /translation="MTQAEVWTIGRLLRWTTEFLQQRGSETPRLDAEVLLAHAHGWER
                     IQLYTHYEDVPGEEVRSRF"
BASE COUNT          670 a          719 c          873 g          593 t
ORIGIN      
        1 gagggcgtcg ttgccaagta cgtttttaac caaatagcgc ccttcatgct gacatccgaa
       61 acagggatca tgccactgtg gaggggaaga atcctccatt tgcgcgggaa tctcgtgggg
      121 gaagaagggg cggcccatta ctatcagttg gcccgtcctc cggaacgcct gattgacagt
      181 gggcagcttg cagcggacga gcagcttatg tacacgatcg cgaaactgaa tgcctcgtat
      241 tggctcgggt tgattgccgt ggatcaggga aatcgaaaaa gtgcggaaga ttacctgatc
      301 aatcggactg caatggtctt accggatcag ccctggcact cgggggtcct ttatgccttg
      361 gggcgactca atgagaagct gggggactac tcgcaggcaa tcacgaccta ccggagtgaa
      421 tttcaactcc cgaaccggtt tggaaatctc gtgcgtgctc gactgatcga agagcttacc
      481 ggcgcaaagc ctctcgaaat cagcccagag cccaccacca acaaggcgga taaggcgtca
      541 caaccggaca agacgtccgg gaagcaagaa taggtcgagg tgggagttta accccaatcg
      601 caggcagtat gcctgtttga ggttgggcgg ttcggcccta gaggctttcg gaagagatga
      661 ccatcgcccg caaggccggg gctttatggt gccaaattcg ctgggtgaag ccccgtttcg
      721 gcagcagtgg cttattgtgg cctttctggc aaaaaggtaa aatcaagttt ttgggacgtc
      781 gccgggcggc cttgtctagg gggctgtcgt ctcgtaagag aatgcggccg agtaagatga
      841 gtgggccagc cccgccttgc agatactggc gagggtacca tcgcgaaacg ttcgttggga
      901 aacgtcctcc caactgtagt aataatgaca cacctgagga ggttgagttg aatgaaagag
      961 ggaattcacc ccaaatatgt tgagtgcaag gtgacatgcg gttgcggtaa cagttttgtg
     1021 acccgtagca tcaagccgga gttgaagatc gacatttgca acgtgtgtca cccctttttc
     1081 accggcaagc tgaagtacgt cgatacgaca ggtcgcatcg agaaatttaa gtcgaaattt
     1141 gcggcaggct attcgagcgt tggtaagaag aaatgatgag cgggaaggga ccatcgtttt
     1201 tgaccgcgtg ggtcgagagt tgcggccggt gatagggttc gtgcttggtt gcgaagaggg
     1261 gtctcttgtg ctcattggac cgatgggtgg gacgtatcgt cccattctgc tgacgttcta
     1321 cttgtatgcg aaaagagctt gacgagaaac ttgcgcgatt tgaggaattg gagcggctcc
     1381 tccatgatcc ggcggtcttg tctgatccaa gccggatggc caccatcatg cgggaacacg
     1441 gttcgttggc caaggtagcc gtcaagtatc gcaagttcaa ggagatcaat cagcagattc
     1501 ttgagcttcg ccaatggatc gaaggggaag atcctgaatt gcgggattta gccgaagagg
     1561 agttgccacg cctccggcag gaacgggaag ccctttggga tgagttactc gagttggcct
     1621 tgggcgggga agatgccaat cgtacccggt gcatcatgga gattcgagcc ggaacggggg
     1681 gtgaggaggc ggccttattt gcccgtgacc tcttcgagat gtaccggcga tatgcggagt
     1741 ccaaggggtg gaagctcgaa atcctcggca tgaatcctac cgaacttggt ggcttcaagg
     1801 aaattagttt tggtctggaa ggagaagagg tttttcgcag gctccagtac gagagcggcg
     1861 ggcatcgggt gcaacgggtt ccggaaaccg aggccaaggg ccgtattcac acctcgctgg
     1921 ccaccgtagc cgtcctgccg gagccggaag acatcgagat cgacatcaag ccggaagact
     1981 atcgccgaga cacgtatcac gccagcggac ccggcgggca gcacgtgaac aagacctcct
     2041 cggcaattcg cctcacgcat tatgcgacgg gcatcgttgt tcagtgtcag gatgaaaaaa
     2101 gtcagcacaa aaattttgct aaggccctgc gggtgctcaa gacccgagtt tatgaatact
     2161 accgacgggc caaagaggcc gagcgggccc aagaacggcg gagcaagatc ggctcggggg
     2221 accgcagtga acggattcgc acatacaatt ttccggagaa tcgcgtgacg gaccaccgga
     2281 tcaatctgac gctttaccag ttggatcaga tcatggaggg aaaccttgat ccactcatcg
     2341 aagccctgct cgattacgag cgtaatgagc tccgtctgac cttcggttcc ctggattgat
     2401 aagctaccaa tagggggcga ttcttgccct ctttgtaccg acgccgcaca ataaaaccct
     2461 atcccaaaaa gcccccctct cccttcggga gagggttggg gtgagggcaa gcgaacgtca
     2521 gaatcgattg agaaaaccgg cagcgattcg agccaccgtc actcccgacc cctcttcccc
     2581 cgagcgggta agggagatta cgggatacgc tctaacgcag gttctgtggt ggccaagcgt
     2641 gtgctggaag atgaaagacg ccgagcatga cgcaagctga ggtttggacc attggccggc
     2701 tcctccgctg gacgaccgag tttcttcagc agcgcggttc agaaaccccg cgactggacg
     2761 ccgaagtcct tcttgcccac gctcatggtt gggagcggat tcaactgtac acccactacg
     2821 aagatgtccc cggcgaggag gtccgtagtc gcttt
//
LOCUS       DAOPTR010000483         2848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_28099_length_2848_cov_5.424633, whole genome shotgun sequence.
ACCESSION   DAOPTR010000483 DAOPTR010000000
VERSION     DAOPTR010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28099_length_2848_cov_5.424633"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="PKI05_09665"
     CDS             complement(<1..1055)
                     /locus_tag="PKI05_09665"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOA52512.1"
                     /translation="MASITSRKDGIRQIQFVTPEGDRKTISLGKCSKRAAEQFARFLE
                     RLLECRRLGVVLDSETTRWLSELPTDMYARLVRLGLVPARAKTIAPALEDFLQGYLET
                     RPDVKPASKLAWQQTMRHLVAHFGADKPIDDITPADAETFRSALLARGLSPATVAKRL
                     SFARQFFQYAVKARLLAENPFTGVKCPKGDPKARQRYISPETTQRLIEACPNVAWRTV
                     VALCRYGGLRCPSEVLSLKWEHVNWERGEIRVPQPKVERHGKPYRVIPIFAPLRPYLE
                     EAWEAAEPGAEYVVPFDVYRQAANHPESWLRSNMRTQFARIIRKAGLTAWPRPFHNLR
                     ASLATDLTASFPSHVVAT"
     gene            complement(1173..1754)
                     /locus_tag="PKI05_09670"
     CDS             complement(1173..1754)
                     /locus_tag="PKI05_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52513.1"
                     /translation="MNPIELLYRPGVAVDWVDGELKLRIRKGALTAEEIQTLQENKPL
                     VGGWLLLRRLWDAGYSLHLERRSAGDGHFIIPRGQRSPDVDIKALFALYDAYHDSAVD
                     LLLQACQLAQIAPEQWHINAHEIVLNDLTEANPPKPTDDEDKPRPICRCGSTEYVDHP
                     IHGGQSLRRDCARCGRFIGFVLWYGVQRPANGQ"
     gene            complement(1751..>2848)
                     /locus_tag="PKI05_09675"
     CDS             complement(1751..>2848)
                     /locus_tag="PKI05_09675"
                     /inference="COORDINATES: protein motif:HMM:NF023726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3631 domain-containing protein"
                     /protein_id="HOA52514.1"
                     /translation="EYGPLTLLADEFDAYGQENEDLRCVFNAGHTRSQAYVIRTVPVG
                     DRFEIRRFSCWGPKAVGMIGEPNRTIVDRAIKITMTRKPSSEGVERLDRPAREALAVI
                     ARKLMALSQDGTLTTALSRAAIAVPRALNDRAADNWRPLLAIAEAAGGEWPALAHRAA
                     VAISCDTEPADIREVFARDVLSIVADYGECQEIRAKEIAELLANMEDSPWPSYRRGVA
                     ISPEQVGRLLRSFGVTCTRVKRGSTYAVGTIREKLLPYTPETTATTATVDVSDELNAT
                     YGVAVSQNPTATPETYCNHVSDELNATYDDGCSSGSRKGGLKHNLVNPTPNHPQDNLF
                     DVSPISGNDASPTGVPTDGSDDDGEWLEITL"
BASE COUNT          536 a          821 c          858 g          633 t
ORIGIN      
        1 gtcgcaacga cgtgggaggg aaaacttgcc gttaggtcgg tggctaggct ggcccggagg
       61 ttgtggaagg gtcggggcca tgctgttagg cccgccttgc ggatgatgcg ggcaaattgg
      121 gtccgcatgt tgctccgaag ccagctttct gggtgattag ccgcttgccg gtacacgtcg
      181 aaggggacga cgtattccgc tcccggttca gcagcttccc atgcttcctc aagatagggc
      241 cgtaaagggg cgaagatcgg gataacccga tacggcttgc cgtggcgctc aaccttgggt
      301 tgcgggacac ggatttcacc gcgttcccaa ttgacgtgct cccacttgag agacaagact
      361 tccgatgggc agcgtaggcc gccgtatctg cacaaagcca ccacggtacg ccaagccaca
      421 ttggggcagg cttcgatgag tcgttgcgtg gtttccgggg agatgtagcg ttgccgggcc
      481 ttggggtcac ccttggggca tttgacgcca gtaaacgggt tttcggccaa gagacgggcc
      541 ttcacggcat actggaagaa ttgacgcgca aagctcaacc gcttggccac cgtggcaggg
      601 gataggccgc gggccagtaa ggcagatcga aacgtctcag cgtccgccgg ggtgatgtca
      661 tcgatcggct tatccgcccc aaagtgtgcc actaggtggc gcatcgtttg ttgccaggcc
      721 agcttggagg ccggcttgac gtcaggtcgg gtctccaaat agccttgcag aaagtcctcc
      781 agggcagggg cgattgtctt tgcccttgct gggactagtc ctagccgcac caatcgggcg
      841 tacatgtccg tgggtaactc cgaaagccac cgcgtcgttt cgctgtccag gaccacgcct
      901 agccgtcggc attccagtag cctttcgaga aaccgggcaa actgttctgc tgcccgctta
      961 gagcactttc ccaacgaaat ggttttgcgg tccccttcgg gagtgacgaa ttggatttgg
     1021 cggatgccat ctttgcgtga tgtgatacta gccatatgtc accctttaac ctaacccaaa
     1081 gaaacccatg ttttgtctct ggaagctacc ctctcgacgt gaaataggcc tttctgtagc
     1141 gtgcctattt taggcgaagc cgcgggcggc ctctattggc catttgcggg ccgctggaca
     1201 ccataccaca gcacgaagcc gatgaagcgt ccacaccggg cacagtcccg ccgcaaggat
     1261 tgaccgccgt gaatcgggtg gtcaacgtac tccgtcgacc cacaacggca gatcggccgc
     1321 ggcttgtcct cgtcatcggt cggcttgggc gggttggctt ccgtcaagtc gttcagcaca
     1381 atctcatggg cgttgatatg ccactgttcc ggggcaatct gggccaattg acatgcctga
     1441 agtaaaaggt caaccgcact atcgtggtag gcgtcgtaaa gggcaaagag ggcctttatg
     1501 tccacgtccg ggctgcgttg tccccgtggg atgatgaaat gcccatcgcc ggcgcttcgc
     1561 cgctccaagt ggagagaata accggcatcc caaagccgcc ggagtaatag ccaaccgccg
     1621 acaagaggct tgttttcctg gagggtttga atctcttcag ccgtcaacgc ccctttgcgg
     1681 atgcgtaatt tcagctcccc atccacccaa tccacggcca ccccgggccg atagagtaac
     1741 tcgatcgggt tcataaggtg atctccaacc attcgccgtc atcgtcgctt ccatccgtgg
     1801 gaacaccggt cgggctggcg tcattgccac ttatggggga tacgtcgaat aagttatcct
     1861 gggggtggtt tggcgtgggg ttgacgagat tgtgctttag accccccttc ctactgccac
     1921 tactgcaacc gtcgtcataa gttgcgttta attcatcact tacgtggttg cagtaggttt
     1981 caggggtggc agttgggttc tgacttactg caaccccata agtggcgttc aattcgtcac
     2041 ttacgtcaac ggttgcagta gtggcagtag tttccggggt gtagggcaat agtttctctc
     2101 ggatggttcc aacggcgtat gtgctgcctc gtttaaccct ggtgcatgtc acgccgaagg
     2161 agcgcaacag ccttcccact tgttcggggg agattgccac cccacggcga taactcggcc
     2221 agggggagtc ttccatgttg gcaagcagct cggcaatttc ttttgctcgg atttcttgac
     2281 actcgccata gtcggccaca attgacagaa cgtccctggc gaacacttcc cggatgtccg
     2341 caggttctgt gtcgcaagag atggccaccg ccgcccgatg ggccagcgcc ggccactctc
     2401 cgcctgcggc ctcggcaatg gccaagaggg gtcgccagtt atcggccgcc cggtcgttta
     2461 gggcccgcgg gacagcgatc gccgcccggc tgagcgcggt ggttagtgtg ccgtcttggc
     2521 ttaacgccat gagcttgcgg gcgatgacgg ccaatgcctc gcgggccggt cggtcgaggc
     2581 gctccacccc ttcgctgctc ggcttgcggg tcatcgtgat cttgatcgct cgatcgacga
     2641 tcgttcggtt cggttcgccg atcatgccaa cggccttcgg cccccagcaa gagaatcggc
     2701 gaatctcaaa acgatcgccc accgggacgg tccgaatcac gtaggcttga ctacgggtat
     2761 gtccggcatt gaagacgcag cggaggtcct cgttttcctg gccataggcg tcgaattcgt
     2821 cggcaagtag cgtcaagggg ccgtattc
//
LOCUS       DAOPTR010000484         2848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_28103_length_2848_cov_3.104547, whole genome shotgun sequence.
ACCESSION   DAOPTR010000484 DAOPTR010000000
VERSION     DAOPTR010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28103_length_2848_cov_3.104547"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..230)
                     /locus_tag="PKI05_09680"
     CDS             complement(42..230)
                     /locus_tag="PKI05_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52515.1"
                     /translation="MLHVISCAAVLIFIRDHQVRRRSCMRLAADEVGTLGATDEQLGI
                     DIIGSGNAGDPKRVANDS"
     gene            complement(785..2038)
                     /locus_tag="PKI05_09685"
     CDS             complement(785..2038)
                     /locus_tag="PKI05_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52516.1"
                     /translation="MSQSKIVKKDNVGKILDQAMDAGGGILRLTPTWVPRSFLHPGRR
                     IKLATEDLYAYGAHRGGIDERWFASTTEAANEGRNWDEGLSWVRFNGERFLLRDAVEE
                     GQGRIVGPAIWEKYHRWPVYSKFFDNAGPIPHHMHQTHEFAKLTGQEGKPEAYYFPPQ
                     LNAAENHFDYTFMGLEPGTTKEDVKRCLENWNKGDNGILDLSRAYRLKPGTGWLIPAG
                     VLHAPGSRCTYEPQWGSDVFGMFQSIVEGRYVPWDLLVKDVPKDKHHDLDFIVSQLDW
                     EKNVDPYFKEHNYLEPIVDEENSGDGYTDRWIVYGLVDGQQLFSAKELTVMPGAKCVL
                     RDPGASGWITVQGCGKIGSLWLQTPIMIRFGEEPDDEVFITYEAAKAGVVVENTGTEP
                     LVSLRYFGPDVHPRVPNVGDYKKAR"
     gene            2350..>2848
                     /locus_tag="PKI05_09690"
     CDS             2350..>2848
                     /locus_tag="PKI05_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52517.1"
                     /translation="MGNVWVCQYCGCRIIVRSAKPAVIKVCPRCGQGSHSSQNAIPPE
                     QSSESFPPLVTESYPGRDRRSKARRRRPFFGSSYGFGLAIAGGMVVIGAAVLAIWLAG
                     GFSGGDGARQPRSSSQPKSHGETIPPPLEWTSRQVFVAKTLDQVKEAVVKIEIPLEGG
                     AAIETG"
BASE COUNT          614 a          829 c          791 g          614 t
ORIGIN      
        1 ccgcggtctt gttttcggac ctcaaatgac cggtagagac gtcaggagtc attagcgaca
       61 cgtttggggt cgcctgcgtt tccgctgccg atgatgtcga tgccaagttg ctcatcagtg
      121 gcccccaatg tccccacttc atctgcggcc agcctcatgc acgaccgtcg ccgaacttgg
      181 tggtcgcgga tgaagatcag caccgccgca cacgaaatca cgtgcaacaa gaggttacgc
      241 tttaacgacc gacaagaata tccctgactg acaagtctgt gaaacacacc ctattaaagg
      301 ctaggtcaga atgggcccgg cgtcaaatga gaagcccgta atcccttttt catccccatg
      361 ggattgcagt gaccacgttt gctatatcgc ttcccaaaaa cgtccggagg ctggcaaagc
      421 ctaccggccc cactaaaggg tggaaatgac tggaagccgc ctttggttcc ttagagccta
      481 tcccataatc ttccctcacc cgttcggcgg gagaggggtc gggggtgagg gtgactcaaa
      541 ccgctgctgg ttttctcaaa cgattctgac gttcgcttgc cctcacccca accctctccc
      601 ggagggagag ggggactttt tggaataggc tcctagccgg gaaaaagacc accctaaccg
      661 gtggtcaagt ggccggacgg acacaaaaaa caaaacgcgc accatggcgt gcgggtcgcc
      721 gagaaatcga attgttacca aacttggttc acattctggc cattccttgt ttggccgccg
      781 gtggtcaacg ggccttcttg taatcaccca cgttgggaac gcggggatgg acatccgggc
      841 cgaagtagcg gaggctcacg aggggttccg taccggtgtt ttccacgacg acaccggcct
      901 tcgccgcctc ataggtgatg aagacctcgt catccggttc ctcgccaaag cgaatcatga
      961 tcggcgtttg cagccagagg cttcctatct tgccacagcc ctggacggtg atccagccac
     1021 tggcacctgg atcgcggaga acacacttgg ccccgggcat gaccgtcagc tctttggcgg
     1081 agaagagctg ttgaccatcc accaagccat agacaatcca gcggtcggtg tagccgtcgc
     1141 cagagttttc ttcatccaca atgggttcca agtagttatg ttccttgaag tacggatcga
     1201 cattcttctc ccagtcgagc tggctgacga tgaaatcgag gtcgtggtgc ttatctttgg
     1261 gcacatcttt gaccaagaga tcccacggaa catatcgccc ttccacgatg gattggaaca
     1321 tgccaaacac atccgatccc cattgaggtt catacgtgca gcgggagccg ggcgcgtgca
     1381 acacacctgc agggatcagc cagcccgtgc ccggcttgag gcgataggcc cgcgagagat
     1441 cgagaattcc gttatcccct ttgttccagt tctctaagca gcgtttgaca tcctccttgg
     1501 ttgtccctgg ttctaggccc atgaatgtgt aatcaaagtg attttccgcg gcgttgagtt
     1561 gcggcgggaa gtaataggcc tcgggcttac cctcctggcc ggtcagcttg gcgaactcgt
     1621 gtgtctgatg catgtgatga gggatgggcc cagcgttgtc gaagaacttc gaatatacag
     1681 gccagcggtg gtacttttcc caaatggcag gcccgacaat tcgcccctgg ccttcctcga
     1741 ccgcgtcgcg caggagaaac cgttcgccat taaagcggac ccagcttagg ccttcgtccc
     1801 agttgcggcc ttcattggcg gcctccgtcg tactggcgaa ccagcgttcg tcgataccac
     1861 cgcggtgagc cccatacgca tacaaatcct cagtggcaag cttgatccga cgaccgggat
     1921 ggagaaaact tcgcgggacc caagtcggtg tcaatcgcag gataccaccg cccgcatcca
     1981 tggcctgatc caaaatcttt ccgacgttgt ctttcttaac aatcttcgac tgggacattg
     2041 caggcgcgct cctccaataa gacctaccct cggcgttgag aataacgcca tcaacggaac
     2101 cgtaccgaac gacgcggacg ccacgccgtc ctaccacgcc acactgatat cgcccatttt
     2161 tatccattgc cccatcgtaa agcaagcact tgagaatctc acccgtagaa ttccgacagg
     2221 actagcgacg ctcaatggag atccatcgac ccgcgccctg ttgctcgttg ccgaccaaat
     2281 gggtggtcag aaccctggcg aggatccagc caaatcggta gtctgaatag gcagagagaa
     2341 tgtgagctca tgggcaatgt gtgggtgtgc cagtattgtg gatgccgtat catcgtccgg
     2401 tcggcgaaac cggcggtgat caaggtttgt ccccgttgcg ggcaagggag tcattcttcc
     2461 caaaatgcca taccaccgga gcaatcatcg gagtccttcc caccccttgt gacggagtcc
     2521 tacccaggac gtgatcggcg atcaaaagcc cgtcgccggc gacccttttt tggctcaagc
     2581 tacgggtttg gtcttgcgat tgccggtggc atggtcgtta tcggggcggc cgtgcttgca
     2641 atctggcttg ccggtggctt ctccggaggc gatggggcga ggcagccgcg gtcatcgtcc
     2701 cagccgaaat cgcacggcga aacgatcccg cctccgttgg aatggacctc gcggcaagtc
     2761 tttgtagcca aaacgctgga tcaagtgaaa gaggccgtgg tgaagattga gattcccttg
     2821 gagggtgggg cagctatcga gacgggga
//
LOCUS       DAOPTR010000485         2848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_28104_length_2848_cov_2.971715, whole genome shotgun sequence.
ACCESSION   DAOPTR010000485 DAOPTR010000000
VERSION     DAOPTR010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28104_length_2848_cov_2.971715"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="PKI05_09695"
     CDS             complement(<1..529)
                     /locus_tag="PKI05_09695"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOA52518.1"
                     /translation="MLAHDPARSGATPVEIRPPFVRKWYRLFPDEGLMAGVQPVVADG
                     IVYVGTLRGTLHAIDDATGEDRWVFRAGGAILHTCAAGDGKVFFGAADGRIYAVRAAD
                     GELVWSVPTNAAVWNAPLFWKGLVVIGSRDGKLYAVEADLGKLRWTAQTGGPILASPA
                     LDANSQRVYVASEDMH"
     gene            complement(804..2795)
                     /locus_tag="PKI05_09700"
     CDS             complement(804..2795)
                     /locus_tag="PKI05_09700"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOA52519.1"
                     /translation="MWLTAASVLVCTLAISPNAKFEPPQLGAARWTDGFWASRFETCW
                     DQTLDALERAMRDPANGAQLSNFEIVGQPGAEHRGNNWSDGDVYKWIEAMARVYAVTR
                     DEALDRKMDEWIEKIARTQADDGYISTQTQFNRHKERWGMRQYHELYNMGHLITAAVI
                     HYQATGKDSFLKVGRKCADYLYTTFQPRPPQLAHFGWNPSQIMGLVDLYRVTGDRRYL
                     ELSEIFINMRGSARWPGPSAFAELAKIGDPHPGDQTQDRVPLRKEEFAVGHAVTGAYL
                     YCGAADVVAETGDKELFAAISRIWKDIVQRKMYITGGTAAYHHGLSIRLDPVHEAYGA
                     AYDLPRRTAYNETCANIAHAMFSYRMLALTGEAQYADIMEQTLYNSVLSGISLDGTKF
                     FYTNPLECRQDAPLLSNDSKERWSTWKCFCCPPNIARTIAGCSQWAWGFSNDGVWIHL
                     FGTGEAQGKCAGDEVHLRQESNYPWEGKITITFVKVPSRSVTLRVRIPAWADGATCQV
                     NDEMPQLCTAGTYHTLARTWKEGDVVRVELPLSVRLLESHPWVEQNRNLVAVMRGPIV
                     YCLESVDLPQGVSIDDVVLPVDSQWQERFEPGLLGGVVALEANAIRLEKSQGNETLYR
                     PVRKGVKPLRIRLVPYYAWGNRGPGDMTVWLPASHTLAD"
BASE COUNT          607 a          880 c          734 g          627 t
ORIGIN      
        1 cgtgcatgtc ctcgctagcc acgtacacgc gctgcgagtt agcatccaag gcggggctag
       61 cgaggatcgg cccacccgtt tgcgcggtcc accggagctt gcccaagtca gcctccacag
      121 cgtaaagttt tccgtcacga ctcccaatga cgacaaggcc cttccaaaag agcggggcat
      181 tccaaacagc ggcgttggtc ggtactgacc aaacaagctc tccgtcggcc gcgcgaactg
      241 cgtagatacg cccatcagcg gctccaaaga acaccttgcc atccccagct gcgcaggtat
      301 gaaggatcgc gccgccggcg cggaagaccc aacggtcctc gcccgttgca tcgtcaatcg
      361 cgtggagggt tccccgtaac gtgccgacat aaacgatccc atcggcaacc actggctgca
      421 caccggccat cagtccctca tcgggaaaga ggcgatacca cttgcggaca aacgggggac
      481 ggatttccac cggtgtggcc ccactgcggg cagggtcgtg cgcgagcatc ggccaatccg
      541 atgaggcgtg ccgctcgtta ccccaaagtg cagcagtggc aactgcccac gagattccca
      601 ctattgctgc tgttaagaag taccctgaac gcatcattca tgccttgtct agctgccagt
      661 tctcgtcaag caagacgatc cttgctggtg acagaaaaca gtcttggtgt tcgcgggctt
      721 tctgaagctc cgctcgcatt cgttttcgat ccctcccgcg atcggttctc ctcttccgtt
      781 gcagccgtgc cttttggctc cattcaatct gccagtgtgt ggctcgccgg taaccaaacg
      841 gtcatatcac ccggcccacg gttcccccaa gcgtagtaag gaacgagccg aattcgcagt
      901 ggtttcaccc cttttcgcac cggccgatat agtgtttcat tcccttgtga cttctccaac
      961 ctgattgcgt tggcttccag cgccacgacg ccccctagta accccggctc aaaacgctcc
     1021 tgccactggc tgtcgactgg caggaccacg tcgtcgatcg aaacaccttg gggaaggtcc
     1081 acggattcca agcaataaac gatggggccg cgcatcaccg ccacaagatt gcggttttgt
     1141 tccacccagg gatgagattc taacagacga actgacaacg gtaattcaac acgcacgacg
     1201 tcgccttcct tccacgtcct ggctagcgta tggtacgtgc cggcggtaca cagttgcggc
     1261 atctcgtcat tgacctggca tgttgcccca tctgcccagg cagggatacg gacccgcaga
     1321 gtcacagagc gactaggtac tttcacaaag gtgatcgtaa tctttccttc ccacggatag
     1381 ttcgactctt gtctgaggtg tacctcatcc ccggcacact tcccttgtgc ttctcccgtt
     1441 ccgaaaaggt gaatccacac gccgtcattg gaaaaacccc atgcccattg gctgcaaccc
     1501 gcgatcgtcc gggcaatatt cgggggacag caaaaacatt tccacgtgct ccaccgctct
     1561 ttggagtcgt tactcaatag gggggcatcc tgacggcatt ctaggggatt ggtgtagaag
     1621 aactttgtcc catcaaggct gatgcccgac agcacactgt tgtaaagcgt ttgctccatg
     1681 atgtcggcat attgcgcttc gccagtcagc gccaacatgc gatagctgaa catggcatgg
     1741 gcgatgttcg cacaggtctc gttgtacgcc gtccgccgtg gcaaatcgta ggcagcacca
     1801 taggcttcgt ggacggggtc aagcctgatg gacagcccat gatgataagc agccgtaccg
     1861 ccggtgatgt acatttttcg ctgcacaatg tctttccaaa tgcggctgat cgcggcgaag
     1921 agctccttgt caccggtttc ggccacaaca tccgcagcac cgcaataaag ataagcacct
     1981 gtgacggcgt gccccaccgc gaactcctct ttgcgaagcg gaacacgatc ctgggtctga
     2041 tcgccggggt gcggatcacc aatcttagct agctcggcaa aggcagaagg cccaggccag
     2101 cgagctgatc cccgcatgtt gataaagatt tcggaaagct ccaagtatcg gcgatccccg
     2161 gtaacgcgat agagatccac cagacccatg atctgtgagg gattccaacc aaagtgggcc
     2221 aactggggtg gtcgcggctg gaatgtggtg tatagatagt cggcacactt ccggccgacc
     2281 ttgaggaaac tgtcctttcc cgttgcttga tagtgaataa ccgcggctgt gatcaagtga
     2341 cccatgttgt aaagctcatg atattgcctc atcccccaac gttccttgtg acgattgaac
     2401 tgggtttgtg tgctaatgta accatcgtcg gcctgtgtgc gggcaatctt ctcgatccat
     2461 tcatccatct tgcgatcgag agcctcgtcg cgcgttaccg cgtaaacacg ggccatggct
     2521 tcgatccatt tatagacgtc gccatcactc cagttatttc cgcgatgctc ggcccccggc
     2581 tgccccacga tctcaaagtt acttaactgg gcaccgttgg ctggatcgcg catggccctt
     2641 tctaaggcat ccagcgtctg atcccaacag gtttcaaatc ggctggccca aaaaccgtct
     2701 gtccatcggg cggccccaag ttgtggcggc tcgaacttcg cattcggcga aatagccagt
     2761 gtacaaacca atacagacgc cgctgttagc cacatcgtct ctctcctaat tcgttttttt
     2821 cctatccgcc taatcctcaa cacccgcg
//
LOCUS       DAOPTR010000486         2839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_28223_length_2839_cov_3.341595, whole genome shotgun sequence.
ACCESSION   DAOPTR010000486 DAOPTR010000000
VERSION     DAOPTR010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28223_length_2839_cov_3.341595"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1764
                     /locus_tag="PKI05_09705"
     CDS             <1..1764
                     /locus_tag="PKI05_09705"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA52520.1"
                     /translation="LGWLGGLLSVFLVLQLGHQVGLNIPWSGVIGPYTLGLSAVVTIL
                     GATIAALWPMLRAGRVPPLEALQPRHANLLHSTNPLKLLPLAALGTVFIMIHPMVVFL
                     LPLKDSFRVWIAITVGAPMAAIGFLCWTPALVVAVEWICSPVLAFVMCVPRQLCKMEL
                     ARNLPRSVATCMALSVGLGVFSAMMVWGYSMLEPFKPTDWAPDMLAAFQSGSVPKQEL
                     QTIASIPGVKRYLPAAVEQPKLAEDLTHASEGASVTRQDNVIIMGVDPDLAFGEDDPL
                     FHLDFVKGSRREIIRRLKSERACIVPDHFVTATGLDIGDTFRVVSPNDPQKTLEYKIV
                     GVVSLPGWHWMTKFSGLRRRYARSAALIFAEESVVRFDFDLKNVNFIWIDLAKNADLE
                     VVCRKLQDVADRHLGEVQPVNIQGTWSFGARMFGRSLRVTLRDEIYQRVTQRADTMIW
                     AMARLPFVTLLITLPGLMSVINASVRIRQWEFGIMRAIGFTSSLIARQIVVEGLLLAT
                     VAAFVSVLFGIVSGWCGIGLSQYTSFFGGMQTPLVIPWRQIAIGVVGTFLICGIAAVP
                     GAIVVLRREPLKLFLAGRGSE"
     gene            2378..>2839
                     /locus_tag="PKI05_09710"
     CDS             2378..>2839
                     /locus_tag="PKI05_09710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HOA52521.1"
                     /translation="MQNRCFWAHSANDGGKGVPERLRDHLERVALRAKEFASSFEAGE
                     QAQLAGILHDLGKYGDRFQQYLEGKVHRAGDHWSAGSLLALKLANKYGECPALAVLAH
                     HSGLNELPGSYKQFWNDLCNCIRRSDEVTESHLDKLWQRLCAEGVPVPTVSS"
BASE COUNT          580 a          723 c          786 g          750 t
ORIGIN      
        1 ctcggctggt tgggtggact cttgtcagtt ttcttggtgc ttcaactcgg gcaccaagtt
       61 ggtctcaata tcccatggag tggtgtcatt ggtccttaca cccttggcct ctcggcggtg
      121 gttaccattc ttggggccac gatcgcggcc ctctggccaa tgttgcgtgc aggtcgggtc
      181 ccgccactgg aggccctaca accgaggcac gccaatctgc tccattcaac gaaccctttg
      241 aaactcctcc ccctcgccgc attggggacc gttttcatca tgatacaccc tatggtcgtt
      301 tttcttttgc cattaaagga ctccttccga gtctggattg ccataaccgt aggggcaccg
      361 atggcggcga ttgggtttct ctgttggaca cccgccctag tcgtcgccgt ggagtggata
      421 tgctcgcccg ttttagcatt tgtcatgtgt gttcctcggc aactgtgcaa gatggaactc
      481 gcacgaaact tgccacgaag tgtggccacg tgcatggcgc tctccgtggg attgggagtg
      541 ttctcggcca tgatggtttg gggctactca atgttagaac ctttcaaacc cacggattgg
      601 gcaccagata tgctcgcggc gtttcagtcc gggagtgtgc caaaacagga actacaaaca
      661 atcgcttcca ttcctggggt gaagcggtat cttcctgccg cggttgaaca acctaagctt
      721 gcagaagacc ttacccacgc atcggagggc gcatctgtta ctcgtcagga caatgtcatc
      781 attatgggtg tcgatccaga tttggctttt ggcgaggacg atccgctctt tcatctcgac
      841 tttgttaagg gttcacgaag ggaaatcatc cggcgcctta agtcagagcg ggcttgcatt
      901 gtgcctgatc attttgtgac agccacggga ttggacatag gggacacttt tcgggtagta
      961 tcacctaacg atccccagaa gacactagag tacaaaattg tcggcgttgt ttctctgcct
     1021 ggatggcact ggatgaccaa gttttccggg cttcgaagac gatatgcgcg ttctgcggct
     1081 ctcatctttg ccgaagagag tgttgtccgc tttgatttcg acctaaagaa tgttaacttc
     1141 atttggattg atctagcaaa aaatgccgac ctcgaagtgg tttgtcgtaa gctccaggat
     1201 gtggccgatc gtcatttggg ggaggtccaa ccggttaaca ttcaagggac atggtctttt
     1261 ggtgctcgca tgtttggccg ttctttacgg gttacactgc gggatgagat ctaccagcgc
     1321 gtgacgcaac gggccgatac gatgatttgg gccatggctc gacttccgtt cgtgaccttg
     1381 ctcattaccc tccctgggct tatgagtgtg atcaatgcct cggtgcgaat ccgacagtgg
     1441 gaatttggta tcatgagggc cattggtttc acttcgagtc tcatcgcccg gcagatagtt
     1501 gtggaaggtc tcctgctcgc cactgtggcg gccttcgtca gcgttctttt cggaatcgta
     1561 tcagggtggt gtggaatcgg tttatcgcag tacaccagtt tcttcggcgg tatgcagacg
     1621 cccctcgtca ttccctggcg ccagatagct atcggggtcg tggggacctt cttgatctgc
     1681 ggtattgcgg ccgttcccgg ggcgatcgtc gtcctccggc gagagcctct caaactcttt
     1741 ctagctggac gtggtagtga gtaagaagcg gcgcctcgtg aatcgtttgc caagcctttc
     1801 accggagttc gattgacatc ttgttggttg cagacgtcgt tggaacactg cgcctgttga
     1861 atgtggtgta agtccagatt tgttacctgc tggaggtcat ccagacaacc gcggcacggc
     1921 tttcttatcc tcccccaagc atttggccgt ttctcggaac gatcccgtcg ctggcaattg
     1981 gcagtgccaa ttcgatgtcg gtgaaaacgc cactctgggc aatctgctat tccacctatc
     2041 acgttttggc acacaagatc cccgcctctt ggaccgccaa gggatgtccc ccacactatg
     2101 caaaatgaaa cgccgtgaaa tcaccatgga aatgatgctg ataggtgcgc gcactctccc
     2161 cccagaagat tctgcggggc tgattgtccg atggagtgtg aacagaagac gatggtcttg
     2221 tctatgacga gactacccgg aggagtccga atgttcttac gctcttgatg agacaaaaca
     2281 cgacgttaca ttgcttcatg agttgggagt tctcgatatt ctcggatcat cggtgttagc
     2341 aagtcattgt tgccaacctg ttttagtgag ggcgaatatg cagaatcgtt gtttttgggc
     2401 acacagtgct aatgacggtg gcaagggtgt ccctgagcga ttgcgagacc atctcgaacg
     2461 tgttgcactc cgtgcgaaag agtttgctag ctcttttgag gcgggtgaac aagcccaact
     2521 tgctggcata ttgcacgact tgggcaaata tggagacagg tttcaacaat atctggaagg
     2581 taaggttcac cgtgctggtg accactggtc ggcaggaagc ctcctcgccc ttaagctggc
     2641 caataagtac ggtgagtgcc cagccctagc ggtcctagct catcacagcg ggctgaatga
     2701 attgccaggg agctacaagc agttctggaa tgatctttgt aattgcatac ggcggagtga
     2761 tgaggtaacg gaatctcacc ttgacaaatt gtggcaacgg ctttgtgctg aaggagtccc
     2821 agtacccacc gtcagcagt
//
LOCUS       DAOPTR010000487         2839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_28224_length_2839_cov_3.110273, whole genome shotgun sequence.
ACCESSION   DAOPTR010000487 DAOPTR010000000
VERSION     DAOPTR010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28224_length_2839_cov_3.110273"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="PKI05_09715"
     CDS             <1..1006
                     /locus_tag="PKI05_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52522.1"
                     /translation="VYAKRLPRKWKLDEPIHEKTLVHGIFLKLASENPESPQPILVTD
                     RPGWFPDNLLGRLGMDCALFDDVVPTRINERFVEASSPATDDTESLLDRLRLTGRDRE
                     CFYQLMAAANRTRPGELFRIAKEELARQQRTASSVVPLFNDPAEQQGKLVLLYGTARR
                     IEEVTVENPDIQKRLGISRYYTIYLFTEDSQNYPVVFCVIHLPPGVTPGEGPRYAVDL
                     ALAGFFYKTWAFRPQRNLSPDAPPHTWQLAPLLIGREALKVTSRAAQNTGVMTFTLLG
                     GVLILIMVVVIVATWFTWREKKRPPFLASSQISAGGEQPAPDFEKMAGPEDRVPNDRE
                     "
     gene            1117..1854
                     /locus_tag="PKI05_09720"
     CDS             1117..1854
                     /locus_tag="PKI05_09720"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009101298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOA52523.1"
                     /translation="MIPIRDTIPSRNVPLTTYFLIFANVLIFLFQLELPEESLQRFFY
                     YFGIVPARYTHPAWAEYVGLPVDNYWPFVTSMFLHGGWMHLIGNMWTLWIFGDNVEDR
                     MRPLRFLLFYLCCGIAAGIVHFLTNPNSTIPTIGASGAISGVMGAYFLLFPHSRVITF
                     IPILFYPVFIALPAVVYLGIWFLTQFLSGVGSLLPGQQVGGIAWWAHIGGFVAGMVLC
                     PLFVERQRPCRGCYPDEYGTELGWMRW"
     gene            1880..2746
                     /locus_tag="PKI05_09725"
     CDS             1880..2746
                     /locus_tag="PKI05_09725"
                     /inference="COORDINATES: protein motif:HMM:NF014074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOA52524.1"
                     /translation="MNVIDFFWIFLMVSALQPVLRQKMIEAARQRVIARIERKRRSRV
                     IVLIHRQETMSLLGFPLMRFIDINDSEEVLRAIQLTDPDIPIDLVLHTPGGLVLAALQ
                     IARAVKMHPAKVTVFVPHYAMSGGTLIALAADEIVMCKHAVLGPVDPQINQQPAASIL
                     RVLQRKNPDEIDDQTLILADIAEKAINQVRMAVSELLAGKMPQEKAEEVARLLCEGTW
                     THDYPLTPETAQQLGLPVSTDMPVEFVQLLQLYPQPRRGQPTVEYIPTPYRYQQPKAY
                     PKTSSLFGRELG"
BASE COUNT          598 a          778 c          761 g          702 t
ORIGIN      
        1 ggtctatgcc aagcgtttgc ctcgaaaatg gaaactcgac gagcctattc atgagaaaac
       61 tttggttcat ggcatcttcc tgaaactggc ctcggagaat ccagaatccc ctcagccgat
      121 cctggtcaca gatcggccag gatggtttcc tgacaatctg ctaggccgat tgggcatgga
      181 ctgcgctctt ttcgatgatg ttgtcccaac acgcatcaac gagcggtttg tcgaagcgtc
      241 gtcacccgca acagacgaca cggagtccct cctcgatcgg ttacgcttaa cgggccgtga
      301 tcgggagtgt ttctatcagt tgatggcggc ggccaaccga acacgacctg gggagctctt
      361 ccgcattgcc aaggaagagt tggcccgaca acagcggaca gcctcctctg tggtgccgct
      421 ttttaatgat cccgcagagc agcaaggcaa actcgttctc ctctacggga cggcacggcg
      481 catcgaagaa gtgaccgtcg agaatcccga tatccaaaag cgattgggga ttagcaggta
      541 ttacaccatc tatcttttta cagaagattc tcaaaattac ccggtcgttt tttgtgtcat
      601 ccatttaccg ccgggtgtga cgccaggtga agggcctcgg tatgccgtgg atcttgccct
      661 tgcgggtttt ttctataaga cgtgggcctt tcgcccgcaa cgaaatctct cgcccgatgc
      721 accgccccat acctggcaat tggccccact gcttattggc cgcgaagctc ttaaggtcac
      781 cagtcgggca gcccagaaca ctggcgtgat gactttcacc ttgctgggag gtgtgttgat
      841 tcttatcatg gttgtggtta ttgtggcgac ctggttcacc tggcgagaga agaaacgccc
      901 accgtttctt gcatcaagtc agatttctgc gggcggggag caaccagctc ccgattttga
      961 aaagatggca gggccagagg acagggtacc caatgaccgc gagtagatca tggggccatg
     1021 gcacaaatgg cttcccagcc tgcacaccgg ttacgtaggg acacaatgaa ctttgatcgt
     1081 gggtaacttg aactctttgg atgaatcaca gcttctatga ttccgattcg agacacaatc
     1141 cccagtcgaa atgttcccct cacaacctat tttctcattt tcgccaacgt gctcattttt
     1201 ctatttcaac ttgaattgcc agaggagtcg ctgcagcggt tcttctacta ttttgggatt
     1261 gtacccgctc gatacacaca tccggcctgg gcagaatacg tggggttgcc cgtggacaat
     1321 tactggccgt tcgttaccag catgttttta cacggaggct ggatgcatct catcgggaat
     1381 atgtggaccc tctggatttt tggggataat gtggaagatc gcatgaggcc gcttcgcttc
     1441 ctactcttct acctatgttg tgggatcgcg gctggcattg tgcatttttt gacgaatccc
     1501 aattccacga tccccaccat tggtgcctcg ggggcgattt ccggtgtcat gggagcttac
     1561 tttctgcttt ttccccattc acgggtgatc acgtttatcc cgattctctt ttatcccgtc
     1621 ttcattgccc tgccggcagt ggtctatctt ggtatttggt ttctcacgca gtttcttagc
     1681 ggtgtgggga gtcttcttcc cggacaacag gtgggaggga ttgcgtggtg ggcccatatt
     1741 gggggatttg tggcgggaat ggtcctctgt cctctattcg tcgagcgcca gcgaccttgt
     1801 cggggttgtt acccagatga gtacggcacc gaattgggtt ggatgcggtg gtaagccttg
     1861 aagggaaagg gctagcggaa tgaacgtcat cgactttttc tggatcttct tgatggtctc
     1921 ggcacttcag ccagttctgc ggcaaaagat gatcgaggca gcacgccagc gggtgatcgc
     1981 ccgcatcgaa aggaaacgcc gttcccgggt tatcgtattg attcaccgcc aagagaccat
     2041 gagtctcctc ggtttccccc tgatgcgatt catcgatatt aatgattccg aggaggtctt
     2101 gcgtgccatc caactcaccg atccggatat ccctatcgat ctcgtccttc atacgcccgg
     2161 tggattggtc ttggctgcct tgcaaatagc ccgggcggta aaaatgcacc ccgccaaagt
     2221 taccgttttc gtcccccatt acgccatgtc gggtgggacc ctgatcgcct tggccgccga
     2281 tgaaatcgtg atgtgcaagc atgccgtgtt gggacctgtg gatccacaga tcaatcagca
     2341 accggcagca tccattctgc gggtccttca gcggaaaaat ccggacgaga ttgacgatca
     2401 aactctgatc cttgccgata ttgcggaaaa ggcgatcaat caggtccgca tggccgtgtc
     2461 ggaactgctt gccggcaaga tgccacaaga aaaggccgag gaagtggctc gcttgctgtg
     2521 cgaaggaact tggacccacg actatcccct cacgcccgaa acggctcagc aactgggatt
     2581 accagtcagt acggacatgc cagtcgagtt cgtgcagctt ttgcaacttt acccacagcc
     2641 ccgccgcggc cagccgacgg tggaatacat ccctactccc taccggtatc agcaacctaa
     2701 agcctatccc aaaacttcct ctctctttgg gagagagttg gggtgagggc aagtgaccgt
     2761 cggagtcgat cgagaaaacc agcagcgatt cgagtcaccc tcacccccgg cccctctccc
     2821 gccgaacggg cgaggggag
//
LOCUS       DAOPTR010000488         2839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_28225_length_2839_cov_2.997486, whole genome shotgun sequence.
ACCESSION   DAOPTR010000488 DAOPTR010000000
VERSION     DAOPTR010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28225_length_2839_cov_2.997486"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1370)
                     /locus_tag="PKI05_09730"
     CDS             complement(132..1370)
                     /locus_tag="PKI05_09730"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOA52525.1"
                     /translation="MLLVLVLCVVFQEVKLGMAGPLLSSAISGEAGEGTGLPFVQEYA
                     VGMMLGLYALRLLVAGRDLSGAASARKVAAGLCVWGTGVILVTVLLGVPVGEGSWGNV
                     RMLVEGLITFFIVSDLGWRRREVWLILVVFVLVACFHAMVVWLSYVNPSLLPFGVTVL
                     DSGGDPRFSGLFLEPSRAGLVFAIAFVLAAANVLDIELARLVRVFWLVGLLVLGVGLL
                     LTQTRGSLLAVAAGMVYLALAEKKLARVGLFVATVGSGLFLWSGSSGVEIVRIGLSRF
                     LADDSAGGGVGRLQVYSFVGEMVARYPLGSGAGALFPESGAQGIPHAHNMYLQMAVMF
                     GIPGAVLFLGFIVATVSSKGAASRSWSGVRSARTLVVLRAALLVNLVALMTEPLLITS
                     VGLWFWVIAGFMVSPSEGLH"
     gene            complement(1481..2542)
                     /locus_tag="PKI05_09735"
     CDS             complement(1481..2542)
                     /locus_tag="PKI05_09735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA52526.1"
                     /translation="MLDSFCQFRTAKPLVSVIMPVWNGEEFVLEAVKSVLNQSYSNIE
                     LIIVNDCSTDGTGAIVSSVRDSRVRILTNTQNRGIAISLLRGIENAQGDYIARIDSDD
                     VCLLSRIGRQVRFLERNPDVDVLGGSALLFGRPPVRLKFASRRDGDIKAELLFRCALI
                     HPSLMFRRSSIDEWYDPQIATAEDYDLWVRLMAGGAKFANLRTPVIRYRLHSKRTSEV
                     RAREQIVESSVAQVKAATFLLGHLTDSESFALRVLSGHHPVCPALLADATNLALQMLR
                     ENDRRLLIEPKALRRRVVYELLLMLSRNLQGIPSLDAVVRLHMLRLVLANPGSVIGAI
                     WYSLLRAPGHFARVSGSES"
     gene            complement(2546..>2839)
                     /locus_tag="PKI05_09740"
     CDS             complement(2546..>2839)
                     /locus_tag="PKI05_09740"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA52527.1"
                     /translation="KLRDFEVPLTAVPYITNYTSELASLYVENSEIVLYRNEGDLLKK
                     LRWLVGDAGKAEEIGLAGYRRALSTHTYERRFEILFDRILRGDSVAEFVKEGG"
BASE COUNT          782 a          953 c          620 g          484 t
ORIGIN      
        1 aaagacctca acgccaagat ccgcgccttc gtcaacggct ggaacgaccg ctgccacccc
       61 tacatctgga ccaacaccac cgaccaaatc ctcaagaaag ccaaccgtca aacaacttca
      121 aacacggccc attagtgcaa accctccgac ggggacacca tgaatccggc gatcacccaa
      181 aaccaaagcc ccacactcgt gatcagcaaa ggctcggtca tcaacgcaac caaattcacc
      241 agcagcgccg cacgcaaaac gaccagcgta cgcgccgacc tcactccgct ccacgatcga
      301 gaggctgcgc ccttagatga caccgttgcc acgatgaacc ctagaaacaa gaccgctccc
      361 gggataccga acattaccgc catttgcaaa tacatgttat gcgcatgcgg tattccctga
      421 gccccagatt ccgggaatag cgctccggcc ccactcccaa gtggatatcg ggcaaccatc
      481 tcccctacaa atgagtacac ttgaagacgt cccacaccgc cgcccgccga atcatcggcc
      541 aggaaccttg ataacccgat tcggacaatt tctacgccac tcgaaccgct ccaaaggaac
      601 aaaccacttc caacagtcgc aacgaacaga cccacccgag ccaacttctt ctccgccagc
      661 gctaagtaga ccattccggc cgccacagcc aaaaggctgc ctcgagtctg agttagcaga
      721 agcccgacac ccaagactaa aagcccgacc aaccaaaaca cccgaaccaa acgtgcaagc
      781 tcgatatcaa ggacattcgc cgcggctaag acgaatgcaa tcgcaaacac cagcccagcg
      841 cgagacggct caaggaaaag ccccgaaaag cgcggatctc ctccactgtc caacaccgta
      901 acaccaaacg gcaagagact cggattcacg taactcaacc agacaaccat ggcgtgaaag
      961 cacgcaacaa gcacgaaaac aacaagaatc aaccaaacct cgcgccttcg ccagcccaag
     1021 tcgctcacga tgaagaaggt gatcaatccc tcaacgagca tccggacgtt cccccatgag
     1081 ccttcaccca cgggaacacc caatagcacc gtgacgagta tgacgccagt cccccagaca
     1141 cataagccag cagccacttt ccgggctgaa gccgctccac tcaaatcacg acccgctact
     1201 agcaggcgga gcgcatacaa accaagcatc atccccactg cgtattcttg aacgaacgga
     1261 agcccagtcc cctctcccgc ctcccctgaa atcgcacttg aaagaagcgg gccagccatg
     1321 cccagcttga cctcctggaa cactacgcag agcaccagca caagaagcat tcgcttcgat
     1381 ccctccgcga agatgggcgt gtctccacta agaccgggtc cagtccacct tcccctggga
     1441 gcagtgcgca accccacgag actcttctcg cgcaccggac tcaactctct gacccgctga
     1501 ctctggcaaa atgcccaggc gcccttaaga gcgaatacca aattgctcca ataacagagc
     1561 cgggattggc caacaccaaa cgcagcatat gcaaacgaac cacagcgtcc aaactaggga
     1621 tcccctgcag gtttcgtgac agcatcaaca ggagttcata cacaacccgg cgacgcaaag
     1681 ccttgggctc gattagaagc cgccggtcat tctcccgaag catctgaagc gccaggttcg
     1741 ttgcatctgc caacaaagcg gggcaaacag gatggtgtcc agaaagcacc cgcagcgcga
     1801 aggactcgga atcagtcaga tgcccgagga gaaatgtggc cgccttcact tgagccacgc
     1861 tggactcgac gatttgctcc ctcgcacgga cttcgctggt tcgcttggaa tgtagcctgt
     1921 agcgaattac aggcgtcctg agattagcaa atttcgcgcc cccagccatc agtcgaaccc
     1981 agagatcgta gtcctctgcc gtcgcaatct gtggatcata ccactcgtcg atggacgatc
     2041 gcctgaacat gagacttggg tggattagcg cgcatcgaaa gagaagttcg gcctttatgt
     2101 cgccgtcacg acgagacgcg aacttcagcc taactggagg acgaccaaac agtaaggcgc
     2161 ttccacccag cacgtcaacg tccggattcc tctctagaaa gcgtacctgc cgcccaatgc
     2221 gcgacaacag acacacgtcg tcagaatcaa tccgcgcgat atagtccccc tgcgcgttct
     2281 cgatccctcg gagcaaacta attgcaattc ccctgttctg cgtattggtc agaatacgca
     2341 ctctagaatc gcgaaccgag gaaacgatcg caccagtgcc gtcagtggag caatcattaa
     2401 cgattatcag ctctatgttc gaatagctct gattcaacac actcttcacg gcctcaagca
     2461 cgaactcctc cccgttccac accggcatga taacacttac caacggcttt gcggtccgaa
     2521 actgacaaaa ggaatccaac acggcttatc ccccttcttt cacgaactct gccacagaat
     2581 ctccccttag gatacgatcg aagagaatct caaagcgtct ttcatacgta tgtgtcgaca
     2641 acgcccgtcg atacccggca aggccaatct cctcagcttt cccagcatcc ccaactaacc
     2701 aacgcagttt cttcagtaga tctccctcat tgcggtacag cacgatttcg gaattctcga
     2761 cgtacagtga agccagttcc gaggtgtagt tggtgatgta cggaacagcg gtcaggggga
     2821 cctcaaagtc acgcagctt
//
LOCUS       DAOPTR010000489         2837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_28255_length_2837_cov_3.538462, whole genome shotgun sequence.
ACCESSION   DAOPTR010000489 DAOPTR010000000
VERSION     DAOPTR010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28255_length_2837_cov_3.538462"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(261..>2837)
                     /locus_tag="PKI05_09745"
     CDS             complement(261..>2837)
                     /locus_tag="PKI05_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOA52528.1"
                     /translation="FEFDGNSRLTVNRITYQFSTAWQRLSINAGLGEDSATIVTGLNS
                     ENIVYTPGRVVVSTPSRSIEVSGIENLTLYASPSAVAEVNDSPADDDSVIFPQRVSIS
                     GGGFSATIVGPQTIHVYARNGGQDAITFYDSPQADQASYKNGVAILQGAGFYNRAKFF
                     ESVTFVGTPGAEDMASLVGTKGSDRLSGRLGDITLTAGGTNVRAVNFWTTIVRSAGGT
                     DTAQLDDSPQDDWVFSFAGDVSLFNMDMTVRVYGFAFTHIYSRYGGFDTAYFYDSPRD
                     DLFVGRQDFSSFRTDPYFSRVKFFDAVYAYSRSGGYDTARLYDSSGNDELTIRPLYVR
                     LEGPGFSNRVETFANVVAYSTGGGTDRVFGYGSDGKDQLTVASSATQFFLAETQILVT
                     ARGFKQEAYSLDATDEVNGAAPRVKINREESSTVIYAIDYFDPSSPTLGFQRAIDALP
                     DEGGVVILPAGVFELRQGLVLRSNVTLRGSDSGTTLRRAPLLNAFVTTEARPGDRRIT
                     VESTAGFRVGDEIALVSQLTTFDTREKTFVIVGIEGNDLILDRPIPEAGYSPTNKAEV
                     VNVFSLVSVNGTVTAPAVNAKIENVRLDGNLRENYRRWRVVLPATLSVVYAANTTIKN
                     VTVVRSPATGIMLEQGHDNLLENCTVENSRRDGISLVWETDSIVRRCTVRGSGYGMAD
                     ISDWGEGILVSGGRDIRVENCLTEYNLGKGLHPAGDLTIGGLWINNVSRYNGGNGFHY
                     CFNNFGIWAVGNELYGNGYYGIGGLGLGGDYGDRFNVVMNNVIYNNQRNGIVVNGGRD
                     NFILNNEIRSNSQLTPGRYAEIVLGPVYTTVVEGNKITPTLNAQAIDAFYVRLFNSIR
                     DL"
BASE COUNT          643 a          780 c          781 g          633 t
ORIGIN      
        1 aacgcggacg aacttagagc ctatcccaaa gttccctctc cccctgggag acggttgggg
       61 tgagggcaag cgaccgttgg agtcgatcga gaaaaccggc agcgattgga gttactctca
      121 tcctcggccc ccctcccgct gaacgggaaa gggcagatcg tgggataggc tctttgccaa
      181 tgacggaggt cgcttatcgt ggacaaaacc aaccagcgta atttgttcac tgtttcttag
      241 tttcggcgag gagattgggt ctaaaggtca cggatggaat tgaagagccg cacgtaaaag
      301 gcgtcaatgg cctgggcgtt caacgtgggc gtgatcttgt tgccctcgac cacggttgtg
      361 taaacggggc cgagaacgat ctccgcgtag cgtccgggtg ttaattgaga gttcgatctg
      421 atttcgttat tgaggatgaa attgtcccgc ccaccgttga ccacgatgcc atttcgctga
      481 ttgttgtata tcacgttatt catgacgaca ttgaatcggt caccatagtc tccgcccaga
      541 ccgaggccac caatgccgta ataaccattt ccataaagct cgttgccaac ggcccaaatt
      601 ccgaaattgt tgaagcagta atggaaaccg ttgccgccgt tgtaacggct gacattgtta
      661 atccacaaac ccccgatggt caaatcaccg gcaggatgca agcctttgcc gaggttgtat
      721 tctgtgaggc agttttccac gcggatatca cgtccgccac tgactaggat cccctctccc
      781 caatcactga tgtcggccat gccgtagccg cttcctcgaa cggtacaacg gcgaacaata
      841 ctgtcggttt cccacaccag actgatgccg tctcggcggg agttttccac ggtgcagttt
      901 tctagaaggt tgtcatgccc ctgctccagc atgatccctg tggccggaga ccttaccacg
      961 gttacgttct tgatggtggt gttggcggcg taaaccacag acagggttgc cgggaggacg
     1021 acccgccacc ttctgtaatt ctcccggagg ttcccatcca atcgcacgtt ctcgatcttg
     1081 gcgttcacag ccggcgcggt aactgttccg ttgacggaaa ccaacgaaaa gacgttgacg
     1141 acttcggcct tgttcgtggg cgaataccct gcttccggaa tgggacggtc gagaatgaga
     1201 tcgtttccct cgatgccgac aatgacaaat gtcttttccc gtgtatcgaa cgtggtgagt
     1261 tgggagacaa gagcaatttc atcgcctacc cggaatcctg ccgtggattc gacggtgatc
     1321 ctgcgatcac caggccgagc ctctgttgtt acgaaggcat taagcagcgg cgcgcgcctg
     1381 agagtggtcc cgctgtcgct cccccgcagt gtgacattcg atcgtagcac caaaccctgg
     1441 cgaagttcga atacacccgc tggcaagatc acgacaccac cctcgtcggg cagtgcgtcg
     1501 atggcacgtt gaaagcccaa cgtggggctg cttgggtcga aatagtcgat cgcgtagatg
     1561 acggttgagg actcttcccg attgattttc acgcgcggag cagccccgtt cacctcgtcc
     1621 gtggcatcca aggagtaggc ttcctgcttg aatcctcgtg ccgttaccag gatttgtgtc
     1681 tctgctagaa agaactgtgt tgccgatgac gccacggtga gctggtcctt tccgtcagac
     1741 ccgtacccaa acacccggtc ggttccgcca cccgtactat atgccacaac gttggcgaag
     1801 gtttccacac ggttggaaaa accggggcct tccaatcgca catacaacgg gcggatggtt
     1861 aactcatcat tacctgaaga atcgtagagc ctggccgtgt cataaccgcc gctgcgggaa
     1921 taagcgtaaa cggcgtcgaa aaacttgacc cgggaaaaat aggggtccgt cctgaacgaa
     1981 gagaaatcct gtcggccgac gaataggtca tcgcgcgggg aatcgtaaaa atacgctgta
     2041 tcaaagccgc cgtagcggga gtaaatgtgg gtgaaggcaa aaccatagac cctcacggtc
     2101 atgtccatat tgaacaggct gacatcgccg gcaaaggaaa agacccagtc gtcttggggg
     2161 gagtcgtcga gttgcgcggt atctgttcca cccgccgacc ggacaatcgt tgtccaaaag
     2221 ttgaccgcgc ggacattggt tccccccgcc gtgagagtaa tgtcgcccaa tcggccgctc
     2281 agacgatcag atcctttcgt gcccaccagt gaggccatgt cctcggctcc cggtgttccg
     2341 acaaaggtca ccgattcaaa gaatttggcg cggttataaa aaccggcacc ctgaaggatt
     2401 gctaccccgt ttttgtaaga cgcttgatcc gcttgagggg aatcgtaaaa ggtgatggca
     2461 tcctgcccac cattccgggc ataaacgtga atagtttgcg gtccgacgat ggttgctgaa
     2521 aagcctcccc cagatatcga aacacgctga ggaaatatga cactatcatc atcggcggga
     2581 gagtcgttga cttctgccac tgcggatgga ctggcgtaga gggtaaggtt ctcgatgccg
     2641 gaaacctcaa tggatcgtga cggcgtgcta acgaccaccc ttcccggggt gtaaacaatg
     2701 ttttcgctgt tcaaacccgt gacaattgtg gcggagtcct caccgagtcc ggcgttaatg
     2761 ctcagccgct gccaggcggt ggaaaactgg taagtgatcc tgtttaccgt gagccggctg
     2821 ttaccgtcga actcaaa
//
LOCUS       DAOPTR010000490         2837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_28256_length_2837_cov_3.389288, whole genome shotgun sequence.
ACCESSION   DAOPTR010000490 DAOPTR010000000
VERSION     DAOPTR010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28256_length_2837_cov_3.389288"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..2714
                     /locus_tag="PKI05_09750"
     CDS             78..2714
                     /locus_tag="PKI05_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52529.1"
                     /translation="MTNHQRHEPTWPFLIILVILFILSATSPRAWRIVERPDPPRPKQ
                     KPELALASAAQRTPHTTKPHESTTERSPLSPIQKDHTQPAAESALGGSSSSVTSFQPD
                     GFSQSREGFSQLDVQQPIGKNGDQSEAPLGMREPTGDLVSELSGNYAQEMAEFAAESH
                     EPCHEAEAPQTVADATDAQAHDAVAKGNAWTVPESLLVLLDSLANVEALRPWVTTVRN
                     QVERLGTAFEQDSLEVALVLDELQRCIDRSSDLQMSLGEDSQLAAQFRQVRYALIRRL
                     ALWKSVHLAGGLNAELAKTHDADWQRLSQKIAALNARLGEGSTAQAWREFLNVEAVSE
                     IARAARANPSDCSKWESQLNQVARDSLARIGRQELTPEQHALLQEPALEDYLNELRFW
                     QGQSLTGAELLRVVEAFETRGLVSDGEAVLSHYYRLSRSSEEPSRWIGQCLNYLYRNA
                     NLRIVITADLLNRLLPQRPREYRPVSETILNRPVYGSSLAETTVAFRLIPDRQRARLA
                     LIVRGEVTSLTYAQAGPATVWNDGFGRYVAEKAIEITPRGLRAEPAVIDVQNDVRLRD
                     VSTSLDSVPVLGFLTQEIARDQVARRRLEANEEARWKLAAQALREIENETESRFEELN
                     ERLARDVLGPLYSLNMSPKWVAAETTNDRIILRIHLGSAMQPGGHTLRPFALSHSLAS
                     CQIHESTVNNFLAQLHLEGQTFSIAELQTKLREKLRLPDLDSPEDNPHAHAQITFAAK
                     DAVRVEFRDGRLNVLLSIARLVSSPNRWENFQVRAIYKPEISHDRIQFVRDGVIQLVG
                     PMDMRSQIALRGVFSKTFSKERPWQVSPGFLEKNPHLADLTVTELTLEHGWLGFSIGT
                     RSAQVNQTANRLPAPSSPPYGS"
BASE COUNT          613 a          846 c          777 g          601 t
ORIGIN      
        1 acggattctt tgccggttga gttccgatcg cccttctcgc aaggagcctt tcggacacgg
       61 atcgaaggaa aggacccatg acgaatcacc agcggcatga gccaacctgg ccgttcttga
      121 ttattctcgt gattcttttc atcctcagtg ccacatcgcc ccgggcttgg cggattgtcg
      181 agcgtccaga cccacctcgg cccaaacaga aaccagagct cgcgctggct agtgctgcgc
      241 aacgtactcc gcataccaca aaaccccatg aaagcactac ggagcgctca ccgctttcac
      301 ccattcagaa agatcacaca caaccggcgg cggagtcagc gctcggcggc agttcctcct
      361 cggtcacctc gttccaacca gacggctttt cccaatcccg agagggtttt tctcaactgg
      421 atgtgcagca accaattggc aagaacggtg atcagtccga agctccgctg ggaatgcggg
      481 agcctacagg ggatttagtc tccgagttgt ccggaaatta cgcccaagag atggcagaat
      541 tcgctgccga gtcacacgag ccgtgccatg aggccgaagc accgcaaacg gttgccgacg
      601 caaccgacgc gcaagctcac gacgcggtcg caaaaggaaa tgcctggacg gttcccgagt
      661 cccttttagt gttgttggac tcgcttgcca acgtggaggc acttcgcccg tgggtgacaa
      721 ccgtccgcaa tcaggttgaa agattgggaa cggccttcga gcaggattct ctcgaggttg
      781 ccctggtcct cgacgaatta caacgatgta tcgaccgatc gtccgatctg caaatgagct
      841 tgggtgagga ttcccaactt gcggcgcaat ttaggcaagt gaggtacgcc ctaatccgcc
      901 gtctcgctct atggaagtcc gtccacctgg caggcggcct caacgccgaa ttggccaaga
      961 cccacgacgc ggattggcaa cgactttctc aaaagatcgc ggccttaaat gctcggttgg
     1021 gcgaaggaag taccgctcag gcctggcgag agttcctcaa cgtggaggcc gtcagcgaga
     1081 tcgcgcgagc tgctagggcg aatccctccg attgctcgaa atgggagagt cagcttaatc
     1141 aggttgcgcg ggattcactt gcccgaattg gtcgccagga actgaccccc gaacaacatg
     1201 cgttgctcca ggaacctgcc ctggaggact acctcaacga actccgcttc tggcaagggc
     1261 aaagtttgac gggggccgaa ctgcttcggg tcgtcgaggc ctttgaaacg cgcggtcttg
     1321 tgtccgatgg cgaagcggtc ctcagtcact attaccggct atcacggtcg tccgaggaac
     1381 catctcgctg gattgggcaa tgtttgaact atctctatcg aaatgccaac ctccgcatcg
     1441 ttatcacggc ggatttgctc aatcgtttgc tccctcagcg tccccgggaa tatcggcctg
     1501 tgtccgagac tatcctcaat cgaccggtgt atggttccag ccttgcagaa accactgtgg
     1561 ccttccgcct gatccccgat cgccaacggg caagactggc cctcatcgtc cggggagagg
     1621 tgacctcgct gacgtatgct caggcagggc cggccacggt atggaatgac ggttttggac
     1681 gttatgtggc cgaaaaggcg attgaaatca cgccgcgagg gttacgtgcg gaaccggccg
     1741 tgatcgacgt tcagaacgat gttcgcctgc gggatgtgtc aacttcgcta gattcagttc
     1801 ctgtgttggg ctttctcacg caggagatcg cccgcgatca ggtcgcccgc cgccgcctag
     1861 aagccaacga agaagccagg tggaagctgg ctgcccaggc cttacgggag atcgaaaacg
     1921 agaccgaaag ccggtttgaa gagctcaacg agcgattggc ccgcgatgtc ttggggccgc
     1981 tttatagtct gaacatgtcg ccgaaatggg tggctgccga aacgaccaac gaccgcataa
     2041 tcctgcgaat tcaccttggc tcggcgatgc aaccaggcgg tcacacactg cgaccttttg
     2101 ccctttcaca ttccttggcg agttgccaaa ttcacgagtc cactgtgaac aacttcctcg
     2161 cccagcttca tttggaaggg caaaccttct ccattgccga gttgcaaacc aaactccgcg
     2221 agaaactgcg actgcccgac cttgattcgc ccgaggacaa tccccacgcg cacgcccaaa
     2281 tcacctttgc ggctaaggat gccgttcgtg tcgagttccg cgacggtcgc ctcaatgtgc
     2341 ttctatcgat cgcgcggctg gtcagttcgc ccaaccgctg ggagaacttc caggttcgcg
     2401 ccatttataa accggaaatc agtcacgacc gtatccaatt cgtccgcgac ggggttattc
     2461 agctcgtcgg cccgatggat atgcgatcac aaattgcctt gcgaggcgtt tttagcaaaa
     2521 cgttttccaa agaacgcccc tggcaggtct cgccggggtt cctcgaaaag aatcctcacc
     2581 ttgccgattt gacagtcacc gaattgacgc tcgaacacgg ctggctggga ttctcaatcg
     2641 gcacacgctc ggctcaggtt aaccaaacgg ccaaccgtct tccggcccct tcatcaccgc
     2701 cttatggctc gtaagatcaa tcattcatgc tgctaacatg aggagtgtta aagtctatcc
     2761 cacaacctcc tctctcccgt tcggcgggag aggggtcggg ggtgagggtg gctcgaattg
     2821 ctgccggttt tctcaat
//
LOCUS       DAOPTR010000491         2837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_28257_length_2837_cov_3.167505, whole genome shotgun sequence.
ACCESSION   DAOPTR010000491 DAOPTR010000000
VERSION     DAOPTR010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28257_length_2837_cov_3.167505"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="PKI05_09755"
     CDS             <1..988
                     /locus_tag="PKI05_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOA52530.1"
                     /translation="ILNLRREVASKYFKQYGVRLDPESEIIVCLGSKDGFSHLCLALL
                     GPGDTAIVPAPSYPPHVYAVILAGANVISLDVTDPERYLSHLEFTCRNLFPRPKLVVV
                     NFPHNPTTTVVEPQFYHDLVKLAKRFGFMIVSDLAYADVTFDGYRAPSLLAVPGAFDV
                     AVELTTMSKGYNMAGWRVGFCAGNSEMIRALATVKTYYDYGMFRPIQIAAIVALRHTD
                     AAVEAQAKEYQRRRDVLCEGLERLGWPIQRPKATMFVWAKIPEPWASQMSSFDFAMKL
                     LKEADVAVSPGGGFGPAGEGYLRLALVENEQRLKQAVRQIGRCLARAGATST"
     gene            complement(1169..1357)
                     /locus_tag="PKI05_09760"
     CDS             complement(1169..1357)
                     /locus_tag="PKI05_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52531.1"
                     /translation="MLTEQQVAKSWYSLFAKGPINEKTIERAESLLRHLRPESPLHYR
                     LSKELEEIRARLMQNAKA"
     assembly_gap    1593..1692
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1984..>2837
                     /locus_tag="PKI05_09765"
     CDS             1984..>2837
                     /locus_tag="PKI05_09765"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOA52532.1"
                     /translation="MADWHRSQFVGAQPHCGPNRLTGLITKQQRLTSGTLWSAVLSLT
                     VCLLSFVGCAERNVYRATRLPLEYQAPIVQDLREIPLTQLVAQQSPSDVIASGDVLEI
                     TINTSLNTKASPVTCRVDDQGMIFVPDIGPVSVGGRRLSDAERLIAETAIARDVYRQP
                     IVNVVRKEGRTYRVTVTGAVEKPGVYHLPASQADLLTALVQAGGLTKQAEPTVEIRRG
                     QNITEVSVPLENPAENAVVEGRLTSFEESTTNTVRIDLISVSTSPASDLRYQLFDGDV
                     IYVPERKV"
BASE COUNT          571 a          786 c          734 g          646 t
ORIGIN      
        1 gattcttaat cttcggcggg aggtggcgtc aaagtacttc aagcaatatg gggtccggtt
       61 agacccggaa tcagagatca tcgtctgtct cggttccaaa gacggattta gccacttgtg
      121 cttggcatta ttaggcccag gcgacacggc aatcgtcccc gccccttctt acccgccgca
      181 tgtgtacgcc gtaattctgg ccggtgccaa tgtcattagt ctcgatgtca ccgacccgga
      241 gcgatatctt tcgcacctgg agtttacctg ccgcaatcta ttccccagac caaaattggt
      301 tgtggtcaat tttcctcaca acccaaccac caccgtcgtc gaaccgcagt tttaccacga
      361 cttggtcaaa ctcgccaagc gttttggctt tatgatcgtc agtgacctgg cctatgcgga
      421 tgtgaccttt gatggttatc gcgcaccgag cctgctcgct gttccagggg cctttgacgt
      481 tgccgtcgaa ttgacgacaa tgagcaaggg ctacaacatg gctgggtggc gggtcggttt
      541 ttgtgccggt aattcggaga tgatccgtgc cctagccacc gtcaagacct actacgacta
      601 cgggatgttt cgccccattc agattgctgc gattgtggct cttcgccata cggatgcggc
      661 tgttgaggcg caagcgaaag aatatcagcg gcgccgcgat gtcctgtgcg aaggcctgga
      721 gcgtctcggc tggccgatcc agaggcctaa ggccaccatg ttcgtttggg ccaagatccc
      781 ggagccctgg gccagtcaga tgagttcctt cgatttcgcc atgaagcttc tcaaggaggc
      841 cgacgtcgcc gttagccccg gtggcggatt cggacccgcc ggagaaggct acttacggct
      901 ggcccttgtc gagaatgaac agcgattaaa gcaagcggtt cgccaaattg gccggtgcct
      961 cgcccgggca ggtgctactt ccacgtagtg cccgtaccta gcgcgcgaag caattcgacg
     1021 ccggcattaa gccccgtatc cgaccgcgct ttgatctcat attgcgcaaa aaaagaaccg
     1081 aaatcggttc tccgcgaaaa tccaaaatgc gaaggccgat ccgctttccc ccgctacgcc
     1141 ttcttttgct cgagccgcgc caaaacaact aagccttggc gttttgcatt agccgggcgc
     1201 gaatctcttc cagctctttt gaaagccggt agtgcaaagg actttcgggc cgaagatgac
     1261 ggagcagcga ctccgcccgc tcaatcgtct tttcgttgat aggccctttg gcgaacaacg
     1321 agtaccaact cttggcgacc tgttgttctg tgagcaagtg tgacctcccg aaaaataact
     1381 acctgagacg gtcccttata acggcggtat taccgtcgga gacggcgcct tttataacat
     1441 tgtacgatcg tcatctccct tgtccatatc ctgtctatta ctatcggtac gtttggtcct
     1501 tcggcttggt ttggaaaaag cctcccattt tccgccccct tcgcctggcc gaatggggga
     1561 gttagagtct atcccaaaaa gtcccctctc ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nncccctctc ccgccgaacg ggagagagaa gattatggga taggctctta
     1741 gtcgcggggt gggcatgccc tcaggttacc gggggtcgct cacagcgctc aatttcgtag
     1801 gttacgaccg cttggtctga ttcccaagcg tgcttttttg atgttgacct atcagcggct
     1861 ttccgctatt tagcgaatcc cattgggacg gcgtgacacc ggcgcgcacc ggtgaacgcg
     1921 tcgccttcct ttctctggag cgagctccaa tactagtttg gatcaaattt aacggaatgc
     1981 cttatggcag attggcatag atcacagttt gtaggcgctc aaccgcactg tggcccaaat
     2041 cgtctgaccg ggcttatcac caaacagcaa cggctgacct ctgggacact ttggtccgcc
     2101 gtgctcagcc tgacagtgtg tcttctctca tttgtcggtt gtgccgagcg gaacgtttac
     2161 cgtgccacac gcctaccctt ggaatatcaa gcccccatcg tgcaggattt gcgcgaaatc
     2221 cccttgactc agttggtcgc tcagcagagc ccaagtgacg tgatcgcatc cggggatgtc
     2281 ctcgaaatta ctatcaacac aagcctgaac acaaaggcct caccggttac ctgtcgggtc
     2341 gatgatcagg gtatgatctt tgtcccggat attggtcccg tttccgtggg tggacgacgc
     2401 ctctcagatg ccgaaagact catcgccgaa accgcaattg ccagggatgt ctatcggcag
     2461 cctatcgtca atgtcgtgcg aaaggagggc cggacgtatc gcgtaaccgt cacgggagca
     2521 gtggagaaac ccggggtcta tcacttaccc gcctcccaag ccgacttact gacggccctt
     2581 gtccaggcgg gcggattgac aaagcaggcc gagcctaccg tggagatccg ccgtggacag
     2641 aacattaccg aggtgagtgt ccccttggaa aatcccgccg aaaacgcggt tgtggaaggc
     2701 cgactgacat ccttcgagga atcaacgact aatacagtcc gcatcgatct catctcagtg
     2761 agcacttcac cggcgtcgga tcttcgctat cagttgtttg atggggacgt gatttatgtg
     2821 ccggagcgga aagtgga
//
LOCUS       DAOPTR010000492         2833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_28304_length_2833_cov_2.834773, whole genome shotgun sequence.
ACCESSION   DAOPTR010000492 DAOPTR010000000
VERSION     DAOPTR010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28304_length_2833_cov_2.834773"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            399..647
                     /locus_tag="PKI05_09770"
     CDS             399..647
                     /locus_tag="PKI05_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008692795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52533.1"
                     /translation="MNGSARKALLALSLLLCGAMGLLTFTTGCQVDVGGQTLPSPYYM
                     KDDVQYFAPGPEFKLSREAAAIQQAKAQQQAQTATVGR"
     gene            809..1201
                     /locus_tag="PKI05_09775"
     CDS             809..1201
                     /locus_tag="PKI05_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52534.1"
                     /translation="MEFPFRLPLLVEYWSYIVLVWLGFGTLIGLLARMVFPGREPSGP
                     LGTVLIGIMGSVIGPLVVETTCGIQGFHPISPLGFVAALGGTAVILVTYRFFLLIIAD
                     SVPARDDQVEETIAPDHVVGVSHPDYYQ"
     gene            complement(1478..2713)
                     /locus_tag="PKI05_09780"
     CDS             complement(1478..2713)
                     /locus_tag="PKI05_09780"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOA52535.1"
                     /translation="MSMFSSHNRVHSLLPVLVFFSALVSLVIEPACAQESKVPGIIID
                     HSPKTSGIYIGSPSLAVLPDGTYVASHDEFGPRSTERTRARTRIFTSSDRGEHWQHVA
                     DVDGQFWSSLFVHDGSLYLLGTWSHYGNIVIRQSKDHGKTWTEPLDSNTGLLRSGQFH
                     CAPVPIVIHNGRLWRAFEDTTDPKRWGLPFRARVISAPVNADLLRADSWTLSEPLPGD
                     PAWLNQRFNGFLEGNIVVTPEGGLVNILRVDCPEGGKAALVTVSPDGKRLSFDPEKDF
                     IDFPGGAKKFTIRFDPQSKRYWSLVNAVPLKYRDRKASSVRNTLVLTSSADLRHWTIH
                     CALLHHPDPVYHGFQYPDWLLDGEDIIAVIRTAFDDEAGGAHNAHDANFLTFHRFRGF
                     RSLTWADSVLKPEEFGIDQ"
BASE COUNT          639 a          763 c          753 g          678 t
ORIGIN      
        1 ttcaggtgat tctttggcct atggtcgggg cgacgcattt gtagcagcac catcatgtcg
       61 tttcctcatc aggacgtggg gaccgcgcga attgtcaggt atctcgctta tcctactcga
      121 agatggcgat aactagcgca ctgtttcggc aggtttcccc aaggcctcca tattacgatc
      181 ttacgctttt ctcctcggga cggccaatga ccgtttaaag tccgctcaat gtaatgacga
      241 taacgattgt gacagttttg ccaacggtca tagagacgca ccaggtagcc gctgtgtggg
      301 gtcattgatg tcgggcggcg aactgttgag tgactcggcc cccgctgcat cgcaattgga
      361 cgggtgtggt tttctccact tgaacatggg gattcaaaat gaacggctca gctcgaaaag
      421 cccttttggc attgtcgttg cttctgtgtg gtgcaatggg ccttttgacg ttcacaaccg
      481 ggtgccaagt tgacgtcgga ggtcaaaccc tgcctagccc ttattacatg aaggacgacg
      541 ttcaatactt tgcccccggg ccggaattta agctctcacg ggaagctgcg gccattcaac
      601 aggcaaaggc ccaacaacag gctcagacag ccaccgtggg ccggtagtcc ggattctcta
      661 acgacatatc gggtcaattg cggaaacgcg ggggtaggga taccttcggt ttccgttttt
      721 ctttgttacc tattgcgcaa gagactgcga cctcgtgaat gtatgccagg ttgcagcatg
      781 ttgaaaaggc cttcggagat cgaaggaaat ggagtttccc tttcgtctgc ctctccttgt
      841 ggaatattgg agctacatcg tcctggtgtg gttggggttc ggcacgttga ttgggttgct
      901 agcccgaatg gtttttcccg gtcgtgaacc gagcggacct ttgggcaccg tcctcattgg
      961 gattatggga tccgtgatag gtcctctagt tgtagagacc acgtgcggta tccaaggatt
     1021 tcatcccatt agtcccctag ggtttgtcgc agccctaggt ggtacggcgg tcatcctagt
     1081 tacctatcga tttttcttat tgatcattgc tgattctgtg cctgctcgtg atgaccaggt
     1141 tgaggagacc atagcacccg atcacgtcgt cggcgtttct catccggact attaccaata
     1201 aggacccagc cgaaggtcgc agatggtcgg caaaaccttc tctcggtcgc gcactttcgc
     1261 gtccgccaaa cgatcggctt taaaaaatcg cggtacacct tggttaccgc gagccaagtt
     1321 ccgcaaactt ggccagaccc cttattgctg atttactctt ggcgaactca caacaactac
     1381 aaggcgggac acgcagtcga tcaccctact gataggccca gaggacccaa tgtcgccagc
     1441 tcgatcgccg agcaaggatc atctgcttgc accactttca ctgatcaatt ccgaactcct
     1501 ccggttttag caccgagtcc gcccacgtca gtgaacgaaa accacgaaag cggtggaagg
     1561 ttaggaagtt tgcatcatgg gcattgtggg ctccgccggc ttcgtcatcg aaggcagtgc
     1621 gaatcaccgc gatgatatcc tcgccgtcca acagccagtc gggatattga aagccgtgat
     1681 aaaccgggtc gggatgatgt aacaacgcgc aatgaatcgt ccagtgtcgc aagtcggccg
     1741 aactcgtcag aaccaacgtg ttgcgaaccg acgaggcctt tcggtctcga tactttaagg
     1801 gtaccgcatt gaccagtgac caatacctct tgctttgtgg atcaaatcga atcgtaaatt
     1861 ttttggcacc gccgggaaaa tcgatgaagt ctttttcggg atcaaacgac agtcgcttcc
     1921 catccggact caccgtcaca agggcagctt ttcctccttc aggacaatca acacgcagga
     1981 tgttgaccaa ccctccctcc ggtgtaacca caatgttccc ttcgaggaat ccgttgaacc
     2041 gctgattgag ccatgccgga tcaccgggca acggctcgct cagcgtccac gaatcggccc
     2101 gaagcaaatc cgcgttcacc ggggccgaaa tgaccctggc acggaaaggt aatccccagc
     2161 gcttggggtc ggtggtgtct tcaaaggccc gccagagccg gccgttgtga atgacaattg
     2221 gaacgggtgc gcagtggaac tgtccgcttc gtagcaggcc cgtgtttgaa tccaaaggtt
     2281 cagtccatgt tttaccatga tcctttgact ggcgaatgac gatgttgccg tagtgcgacc
     2341 atgtgccaag taggtaaagg ctcccatcat gtacaaatag cgatgaccaa aattgcccat
     2401 ctacatcggc tacatgctgc cagtgttcac ctcgatcgct ggacgtaaaa atccgtgtcc
     2461 gtgcccgcgt tcgctccgtc gaacggggac cgaactcgtc atgacttgcc acgtacgttc
     2521 catcgggcag gaccgctaaa ctgggcgagc caatgtaaat ccccgacgtc ttgggtgagt
     2581 gatcgataat aattcccgga accttggact cctgcgcgca agcgggctca atgacgagcg
     2641 acacaagggc gctgaaaaag accagtactg gaagcaaact gtggacacgg ttgtggcttg
     2701 agaacatact catggagcac caatcccttt tgctaatacc tcgttaagcg gcggaagtgt
     2761 cagaaaatcg acaggttctg gtttgtcgcc atctggagta cagaccttca ggaagcgact
     2821 tgccattttc aga
//
LOCUS       DAOPTR010000493         2832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_28316_length_2832_cov_3.329132, whole genome shotgun sequence.
ACCESSION   DAOPTR010000493 DAOPTR010000000
VERSION     DAOPTR010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28316_length_2832_cov_3.329132"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PKI05_09785"
     CDS             complement(<1..547)
                     /locus_tag="PKI05_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009094507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA52536.1"
                     /translation="MRSLSLVLSLVLSASGLGFSTGHAAEAPRPNIIIILSDDMGFSD
                     LGCYGGEIQTPNLDRLAANGLRFTQFYNTARCCPTRACLLTGVYPHQAGVGHMMEDRG
                     YDGYRGDLNRQTVTIAEVLRTAGYRNYAVGKWHVTKATKPEGPKDNWPLQRGFDRFYG
                     TITGAGSYFDPGTLTRDNTMIS"
     gene            841..1830
                     /locus_tag="PKI05_09790"
     CDS             841..1830
                     /locus_tag="PKI05_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52537.1"
                     /translation="MMTGVLSLDHLVVADWLSWLQTLIAMLPVWMKPLWIVLVGFLAG
                     FFLLWVVLQLMAQFAPRVTAIMRTTSKEATSQPLFYILLAIGVTMLLLSPIMPYNTFG
                     EDIKLLKAEGLTLIKLLAIILAVWTASVSISEEVEGRTALTVLSKPVARWKMLVGKFL
                     GVILPVIVMFLILGSLLLCAVSYKVKYDAKEVARPDPTVQECRDEMLQTVPGLGLAFL
                     EAVVMASIAAAISTRFPLIPNLLICASIYALGHLLPTVVIETADRLEFVAFVAKLLAV
                     ILPGLEFFSMETAIATGRSVSASYLGLAALYSLVYISLALLLGLLLFEDRDLA"
     gene            2147..>2832
                     /locus_tag="PKI05_09795"
     CDS             2147..>2832
                     /locus_tag="PKI05_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52538.1"
                     /translation="MSHVTEIKTFDELVGHRLVWRKLWGETPNASFFQSYEWFETYWQ
                     HFGKEQTLRVLVVHSCDEVIGLLPLVVRTERTRVGPVRVLTYPLHDWGSFYSPIGKNP
                     TATLLAGLRYVKESSRDWDILDLRWVDRFGQDRMRTPTAMRQVGFEPSEQVWMQSAIV
                     SIDESWESYWASRTSKFRNNIRRAEKRLAAMGKVEFHRYRFQGEPDEDPRWDLYEICH
                     NLSLRGWQSK"
BASE COUNT          584 a          773 c          780 g          695 t
ORIGIN      
        1 gggagatcat cgtgttatcc cgggttaagg tgccgggatc gaaataactc ccggcgcccg
       61 tgatggtccc gtaaaagcgg tcgaatccac gttgcagcgg ccaattgtct ttgggacctt
      121 ccggcttggt ggccttggtg acatgccact tgccaacggc gtaattgcga tatcccgccg
      181 tgcggagaac ctccgcaatg gtcaccgttt gtcgattgag atcgccacga taaccgtcat
      241 agccgcgatc ctccatcatg tggccaacac ccgcctgatg ggggtaaaca ccggtcaaaa
      301 ggcaggcccg cgtcggacag catcgggctg tgttgtagaa ctgggtaaaa cgcagcccat
      361 ttgcggccag gcggtccagg ttcggtgttt ggatttcacc accataacag cccaaatccg
      421 aaaaccccat gtcatcggaa aggataatga tgatattcgg tcgcggcgcc tccgcggcgt
      481 gccccgtgga gaaacccaat ccgctggctg agaggacgag cgacaaaact agcgaaagcg
      541 atctcatggt tgacctcccc aagtaactga aacggccgaa ccggcaacaa aaaatcaatt
      601 tttgattgac gcactagcga ccttttctcg cttatggttg tcattttgca catttccaag
      661 atcaaaacaa gtcaatcgct agcagaaaag cgttttccga accagaatag acgttgtggc
      721 gtagcgttgt tcgggactcc gggagaagtt tacccatcgg agggcctgat ttaggcgtcc
      781 cggcaagtgc gtcttcgttt tggaatatgg caaggcaagg tcgataactg gatctcgacg
      841 atgatgaccg gagtgttatc actggatcat ttagtcgttg ccgactggct gagttggctt
      901 caaacgttga ttgccatgct tccggtgtgg atgaagccac tctggattgt gctggtcgga
      961 ttcctcgccg gatttttcct tttgtgggtt gtgctgcaat tgatggcgca gttcgccccg
     1021 cgggtcacgg ccattatgcg aacaaccagc aaggaagcca cctcgcagcc ccttttctac
     1081 atcctcttgg cgatcggcgt gaccatgctt ctgctgtccc ccatcatgcc gtacaatacg
     1141 ttcggcgagg acatcaagct ccttaaggcg gaagggctca cgctcatcaa gcttttggcc
     1201 atcattttgg ccgtgtggac tgccagcgtc tcgatctcgg aggaagtgga gggccgaacg
     1261 gcgctcaccg tcctcagcaa gcccgtcgcc cgctggaaga tgttggtcgg caaatttctc
     1321 ggcgtgatac tcccagtcat tgtgatgttc ttgatcttgg gcagtctcct tctatgcgcc
     1381 gtgtcgtata aggtcaagta cgatgcgaag gaagttgcgc ggcctgatcc cacagttcag
     1441 gaatgccgcg atgagatgtt gcagaccgtg ccgggattgg ggcttgcctt tttggaggcc
     1501 gtcgtcatgg cctcgattgc ggcggctatt tccactcgat tcccattgat ccctaaccta
     1561 ctgatttgtg caagtatata tgccctaggc cacctgctgc ccaccgtggt aatcgaaacg
     1621 gctgatcgtc tcgagtttgt ggcgtttgtg gccaaactgt tggccgtcat cttgcctggg
     1681 ctcgagttct tcagtatgga aacggcaatt gccacggggc gaagtgtttc tgcctcctat
     1741 ttgggactgg ccgctttgta cagtcttgtt tacatcagtt tggctttgct cctcggcctg
     1801 ctgttgtttg aagaccgcga cctcgcgtag ctccagccat atatcgccac gcaccgttgc
     1861 aagcctgcgt cggaaatacc gccgatttgt ggtgagatgt ctcttcacat catgaccccg
     1921 gtgtgtgttg ccaattgtca tcatcgtttc tgtccccggg tggtccggca ctgttgatac
     1981 gccttcgcca tttgcaccaa tccgcggcta aatcgtgatt tgccgcaaat cctcggtctt
     2041 ttccgctggg cacgtcgccc catcaccata cggcactgga cttgcgatca tagatactgg
     2101 cgggctaatc ctttccagaa tgtgaccccg tcgcaaggcc acaatcatgt ctcacgttac
     2161 cgaaataaaa acttttgacg aacttgtggg acaccggctc gtgtggcgca agctgtgggg
     2221 cgagacgccg aacgccagct tcttccaaag ttacgaatgg tttgagacct actggcaaca
     2281 tttcggaaaa gagcagacgc ttcgtgtcct cgttgtgcac tcgtgtgacg aggtcatcgg
     2341 cttgttgccg cttgtggttc gtaccgagcg gacgcgcgtc ggacccgttc gcgttctaac
     2401 ctatccgctt cacgattggg gcagcttcta cagccctatt ggcaagaatc ccacagcgac
     2461 gctattagct gggctccgct acgtcaaaga atcctcccgc gactgggata tcctcgattt
     2521 gcgttgggtc gatcgctttg gtcaggatcg catgcgaacg cccaccgcta tgcgacaggt
     2581 aggtttcgag ccctccgaac aggtgtggat gcaatctgca atcgtcagca ttgatgaatc
     2641 atgggagtca tactgggcca gtcgaacgag caaattccgg aacaacatcc gccgggcaga
     2701 aaaacgtctt gcggcgatgg gaaaagttga gttccatcgt taccgtttcc agggagagcc
     2761 tgatgaggac ccccgctggg atctgtatga gatttgtcac aacctctcgc tgcgagggtg
     2821 gcaatcgaag ca
//
LOCUS       DAOPTR010000494         2831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_28323_length_2831_cov_3.141571, whole genome shotgun sequence.
ACCESSION   DAOPTR010000494 DAOPTR010000000
VERSION     DAOPTR010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28323_length_2831_cov_3.141571"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..2223)
                     /gene="ligA"
                     /locus_tag="PKI05_09800"
     CDS             complement(220..2223)
                     /gene="ligA"
                     /locus_tag="PKI05_09800"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOA52539.1"
                     /translation="MGSNVAQEIEKLRDLIRYHDYKYYVEANPEISDLEYDRLMQRLR
                     ELEAAHPELITPDSPTQRIGDRPVEGLEQVRHRIPMLSIDNTYSPQEVRSFAQRVQKL
                     LKGETVEWVVEPKIDGVAVSITYEAGQLVRGVTRGDGFVGDDVTHNIRTVRGVPLRLL
                     TEAPPAVLEVRGEVFMTNSDLVRVNEEQKARGEAPFANPRNATAGSIRLLDPRLCAQR
                     RLRFLCHGLGYSEGFEVDTHMEFLHTVEKWGIPIPPWVTCFADIEKVLAYCEEQIERL
                     HELDFEIDGLVIKVNRFDQRERLGTTAKSPRWVMAYKFEKYEAKTRLLDIRVQVGKTG
                     VVTPVADLEPVTLAGTVVSRASLHNADEIERKDIRIGDVVVVEKAGKIIPHVVRVEKH
                     ERTRPLPKFHFPTTCPECHSTLVRDEGGVYIRCPNHECPAQIKERLRYYASRDAMDIE
                     GLGEKLIDQLVDRGLVRTYADLYRLKKDQLLGLERMGEKSASKLLANIEASKNRGLAR
                     LLAGLAIRHVGAKVAALLAKHFPTIEKLSAATVDELAEINEIGPIIAQSVYDFLHSER
                     GRRIIAELKECGVKMEEEIAVSRQSQPLAGKTIVVTGTLKRYSRHEIEELIERLGGHA
                     ASSVSRKTDFVLAGENPGSKLDKARSLGIPVLSEEEFEKLIAG"
     gene            complement(2438..2821)
                     /locus_tag="PKI05_09805"
     CDS             complement(2438..2821)
                     /locus_tag="PKI05_09805"
                     /inference="COORDINATES: protein motif:HMM:NF018088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOA52540.1"
                     /translation="MKVVHYESIPQQDVTMDGAKGCKIRHLISEADGAPHFAMRQFEV
                     APGGHTPHHKHAYEHEVFVLEGSGVVLQGEREFAIGPGSVVFVPANQVHQFRATGDRP
                     LKFLCLIPHAIQGSNVCCVVACSCD"
BASE COUNT          637 a          869 c          695 g          630 t
ORIGIN      
        1 caccctctaa agtacttacc ggggcattac ggtgtcaagc aataggcgtt ttggataact
       61 ccggtttata ccgcacctac cattcaggtt ccatccccag cttacgccgc agactcgaga
      121 ctcttggctt atacacggcc aaaaaatagt ctctcaaacg cccccgttca aaaccgtaac
      181 agcctctagc gaagggtaat agggggcact ggtgtaccgc tagcccgcga tgagcttttc
      241 gaactcttct tcactgagaa ccgggatacc caacgaacgg gccttgtcca acttgctccc
      301 ggggttctct ccagccagga caaaatccgt tttccgcgag acactagacg ccgcgtggcc
      361 acctaagcgt tcaatgagct cctcaatttc gtggcgcgaa taccgcttca gcgtgcccgt
      421 caccacaatc gtcttgcctg ccaatggttg cgactgccgc gagactgcaa tctcttcctc
      481 catcttcact ccgcactcct ttaattccgc aatgatgcgg cgcccacgtt cactgtggag
      541 aaaatcgtaa acgctttggg cgatgatagg cccaatctcg ttgatctccg ccaactcgtc
      601 gaccgttgcc gccgaaagct tttcgatcgt gggaaaatgt tttgccaaaa gcgcagcaac
      661 cttagcacca acatgacgaa tggccaaccc cgccaaaagc cgtgccaagc cgcggttctt
      721 gctggcctcg atgtttgcca agagcttact ggccgacttc tcccccattc gctccaagcc
      781 caggagttgg tcctttttca accgataaag gtcggcgtac gtccgaacca gtccccgatc
      841 taccaactga tcaattagct tttcgcccag accttcaata tccatcgcat cacggctggc
      901 gtaatagcgc aatctttcct tgatctgagc gggacactcg tgattgggac accgaatata
      961 aacaccacct tcatcccgca cgagcgttga atgacattcc ggacacgtgg taggaaaatg
     1021 gaacttgggc aggggccgag tcctttcgtg cttctccacc cggaccacat gaggaatgat
     1081 cttgcctgcc ttttccacaa caactacgtc gccaatacgg atatcttttc gctcaatctc
     1141 gtcggcattg tgcaggctgg cccggcttac caccgtgccg gccagggtca cgggttccaa
     1201 atcggcaaca ggagtgacca ccccagtctt tcccacttgg acccgaatgt ctagcagccg
     1261 ggtcttcgcc tcgtacttct caaacttgta cgccatgacc cagcgaggac tttttgccgt
     1321 cgtccccaaa cgctcacgct ggtcaaagcg attgacctta atgacgagcc catcaatttc
     1381 aaaatccagc tcgtggagac gttcgatctg ttcctcgcaa taggccaaga ctttctcgat
     1441 gtcggcaaaa caggtaaccc acggaggaat gggaataccc cacttttcca ccgtgtgcag
     1501 gaactccata tgggtgtcaa cttcaaagcc ctctgagtag cccagcccat ggcagaggaa
     1561 tcgcaggcgt cgctgggcac acagacgcgg gtcgagtagt cgaatgctac cggccgtggc
     1621 gttgcgcgga ttggcaaaag gggcctcacc gcgggccttt tgctcttcgt tcactcgaac
     1681 caggtccgag ttcgtcatga atacttcgcc gcgaacctcc aacaccgcgg gcggtgcttc
     1741 agtaagaagc ctgagcggta ctccccgcac ggtgcggatg ttatgcgtca catcgtcacc
     1801 cacaaaacca tcgccgcgcg tcacgccgcg gaccaattgg ccggcctcat aggtaatcga
     1861 gacggctacg ccatcgatct ttggctcgac cacccattcc acggtttcgc ccttgagaag
     1921 cttctgcacc cgttgggcaa agctcctgac ctcttgcggg ctataggtgt tgtcgatcga
     1981 cagcatggga atccgatggc gaacctgttc gagaccttcg acaggtcgat ccccgatgcg
     2041 ttgtgtggga ctgtcagggg taatcaactc cggatgggcg gcttccaact cccgcagtcg
     2101 ctgcatgagc cggtcatatt ccagatcaga aatctctgga ttggcttcca cgtaatactt
     2161 gtaatcgtgg taccggataa ggtcgcgaag tttttcgatc tcttgggcaa cgttcgatcc
     2221 catacgagca ggcctaatct ctcacaagtt cttctcttgc cgtgaatagc tcgccgtccg
     2281 agttgggcgt aatgacatcc ttgaatttac cggccccaaa cttgcgaaga aacagggaga
     2341 agggtttcgc tgctaacttc tcaccgctgg ccgaaccgaa accttaccac aaaaaatcgt
     2401 gcgacgttta gaccgaccca ggtcaccatc ggcgcgctca gtcacaacta caggccacca
     2461 cacagcacac attggaccct tgaattgcat gggggatgag gcagaggaac ttcaacgggc
     2521 ggtcacccgt ggcgcgaaac tgatgcacct gattagccgg cacgaagacg acgctgccag
     2581 gacctatcgc gaattcacgt tctccctgaa ggacaacccc tgacccctcc agaacgaaga
     2641 cctcatgttc gtaggcgtgc ttatggtgcg gggtatgacc accgggtgcg acctcaaact
     2701 gtcgcatggc aaaatgtgga gcgccgtccg cttcgctaat gagatggcgg attttacacc
     2761 ccttcgcacc atccatcgtg acatcctgct ggggaatgct ttcgtaatgg accactttca
     2821 tcgaatctca c
//
LOCUS       DAOPTR010000495         2825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_28406_length_2825_cov_3.272563, whole genome shotgun sequence.
ACCESSION   DAOPTR010000495 DAOPTR010000000
VERSION     DAOPTR010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28406_length_2825_cov_3.272563"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2065)
                     /locus_tag="PKI05_09810"
     CDS             complement(<1..2065)
                     /locus_tag="PKI05_09810"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOA52541.1"
                     /translation="MPNRLIHEKSPYLRQHANNPVDWYPWGEEAFRRAKELDRPIFLS
                     IGYSACHWCHVMEKESFADPEVAAVLNQHFVSIKVDREEHPAVDAQYMEAVQIITGQG
                     GWPLSVFLTPDLQPFYGGTYWPREARFGMPGFLDVLRAIADAWEKRRHEVVAEADKVT
                     VLLSDISNTLPGRHLLSEESVLQKAEQVLAEEFDPRWGGFGVAPKFPHPSVIRYLLTR
                     FAFQDSQMALTTGLRDLDAMADGGMYDHVGGGFHRYSTDAMWMVPHFEKMLYDNALLA
                     RAYLDAWRLTRRERYGQIVNETLDYLLRDMHHPDGGFYASQDADSEGHEGQFYLWTYE
                     EILNVLGPAAERFAEFYDITREGSFEGKNIPNRIRSANRWTALSVGERAQLEQEMAEL
                     RQRLRETRQMRTPPDHDDKIVLAWNAFAAEVFLLAGRVFDEERYLTAGTMALEFLLSH
                     LRDERGMFCHSWCQGVRGTPAVLEDEAALAVALITGYETMARRDWLEIAVTLVEDMRA
                     RFHDAATGVFFMTPADHPHLLCRRLDYFDNPTPSGTALAAEAMLRLGHLGGEPSYLAI
                     AEGALRSLAGWMQKSPIAVGHALFLRQTMVRPWTSWMFVLPSGEVGAVHEGKVPPPVS
                     RWLSSLMSGYNPSMIVSINWLGDGSPSSATDGLPGTKSLPDRPAVNGEPTLYICQGAS
                     CGAPIV"
     gene            complement(2489..>2825)
                     /locus_tag="PKI05_09815"
     CDS             complement(2489..>2825)
                     /locus_tag="PKI05_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52542.1"
                     /translation="AYTPPPPREPPRTPPRTPPRTPPPEPPPLDPIQFVYVNGIVEVN
                     GQREVWLYSRTEDKTFKLTENQTFQVGKTQGRVLRIGIRDVEVEINGQTRTLTLGDNL
                     LGKPISATG"
BASE COUNT          593 a          847 c          809 g          576 t
ORIGIN      
        1 ccacaattgg cgctccacag cttgcacctt ggcagatgta gagggttggt tcaccgttga
       61 cggcagggcg atcgggaaga gactttgtgc cgggtagccc gtctgtggca gaacttggac
      121 taccatcccc taaccagtta attgacacga tcatggatgg gttataccca ctcattaagg
      181 aggataacca gcgcgacacc gggggcggca cctttccctc atggacggcg ccaacctccc
      241 ctgagggtag gacgaacatc caactcgtcc aggggcgaac cattgtttgt ctcaaaaaca
      301 gggcgtgacc aacggcgatc ggggattttt gcatccaacc ggcaagggaa cgcaaagccc
      361 cttcggcaat cgccagatag gagggttccc ccccaaggtg ccccaatcgc aacatggcct
      421 cggcggccag ggccgtcccg ctaggtgtag ggttatcaaa gtagtctaaa cgacgacaca
      481 aaaggtgggg atggtctgcc ggcgtcatga agaagacccc tgtcgcggca tcgtgaaaac
      541 gtgcccgcat gtcttccaca agggtcacag cgatttccag ccaatcccgc cgggccattg
      601 tctcataacc ggtaatgagg gccacggcca gggcggcttc gtcctcgagg acggccggtg
      661 tcccccggac cccctggcac cacgaatgac aaaacatgcc gcgctcatcc cgcaggtgcg
      721 aaagaaggaa ctccagggcc atggtccctg ccgtcagata acgctcctcg tcgaacaccc
      781 gtcctgccag gaggaatacc tccgccgcga acgcgttcca cgccaagaca atcttgtcat
      841 cgtggtccgg tggcgtgcgc atctgccgcg tttctcgaag ccgttggcga agctcggcca
      901 tctcttgttc gagctgagct cgctctccta ccgagagcgc agtccaacga ttggctgagc
      961 gaattcggtt ggggatgttc ttgccttcaa atgacccctc ccgcgtgatg tcgtaaaact
     1021 cggcgaagcg ctccgccgca ggccccagca cattcaggat ttcttcatag gtccaaaggt
     1081 aaaactggcc ctcgtgcccc tcactatcgg catcctgcga ggcatagaaa ccgccgtccg
     1141 gatggtgcat atcacggaga agataatcca atgtttcgtt gacaatttgg ccgtagcgct
     1201 cacgccgtgt caatcgccag gcgtccaaat acgcccgggc caagagggcg ttgtcataca
     1261 acatcttttc aaaatggggc accatccaca tggcgtcggt agaatagcga tggaacccac
     1321 cgcccacatg gtcgtacatg cccccgtcgg ccatggcatc gaggtcgcgt agccccgtgg
     1381 tcaaagccat ctgggaatct tggaatgcga aacgggtcag gaggtagcga attaccgagg
     1441 gatggggaaa ctttggggcg acgccaaagc cgccccagcg gggatcgaat tcctccgcca
     1501 aaacctgctc ggccttttga aggacacttt cctcagatag taggtgcctg ccggggaggg
     1561 tattcgagat atcgctcaat aggacagtaa ccttgtccgc ctccgcaacg acttcatgcc
     1621 gacgcttttc ccacgcatcg gcaattgccc gcaggacgtc taaaaagccg ggcattccaa
     1681 atcgtgcctc ccggggccaa tacgtcccgc cgtagaacgg ttgcaagtcg ggcgtgagga
     1741 acaccgacaa cggccagccc ccttggcccg taatgatctg cacagcctcc atgtactggg
     1801 catcgaccgc cgggtgttct tctcgatcga ctttgataga cacaaagtgc tgattgagga
     1861 cggccgcaac ttccggatcg gcaaagcttt ccttctccat aacgtggcac caatggcagg
     1921 cggaatatcc gatggagagg aagatcggtc gatccagttc ttttgcccgc cgaaaggcct
     1981 cctcacccca gggataccaa tccacgggat tattcgcgtg ctgtcgcagg tacgggctct
     2041 tttcatgaat cagccgattg ggcattggct ttcgctttcg cctccatgtt gtggtaaccc
     2101 cggatacttg ccctccgcca attttccctt ccctgttagc cttttcgggg cgattgccaa
     2161 ccggcacaac cgatccgaaa agcgctgctt cgcggcaggg ggcctgtcca gttaagacgg
     2221 ggcggcatga cctcggcggt accgaacact ctcgttggga ccagagtatc cgaaaagcca
     2281 cggcaaccgt atcaaattca tcatagtcat ttggcccgtg ggggctaggg tccgcagccg
     2341 gcaccggctc ttcttgccgc ggtaagagca accggcctga ccgattcaca ccatgccggg
     2401 tttccgtgcg tgctaagtgt gcgtcaacgt cggtatcccc taagacactt tggtgaggcg
     2461 tgagcggcga ttgctcccag aaagccgatc acccggtggc ggagatgggt tttccaagaa
     2521 gattgtcacc gagggtgagc gttcgcgtct gcccattgat ttccacttcc acatcacgaa
     2581 taccgatgcg gaggacccgt ccctgggtct tgcccacctg gaaagtctgg ttttccgtga
     2641 gcttgaacgt cttgtcttcg gtgcgagaat agagccacac ttcccgctgg ccgttgactt
     2701 ctacaatgcc gttcacatag acgaactgaa taggatcaag cggcggaggt tccggcggtg
     2761 gggtccgggg cggtgtccgc ggtggtgtgc gaggtggttc ccgcggcggt ggcggtgtgt
     2821 aggcc
//
LOCUS       DAOPTR010000496         2824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_28417_length_2824_cov_3.308053, whole genome shotgun sequence.
ACCESSION   DAOPTR010000496 DAOPTR010000000
VERSION     DAOPTR010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28417_length_2824_cov_3.308053"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /locus_tag="PKI05_09820"
     CDS             <1..1422
                     /locus_tag="PKI05_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52543.1"
                     /translation="WDEIAMQSWQLAQEALKQSDDSLTAHDAMPNSGELLSQLSDAIR
                     RVASALVELLPRSRDATSVGTLVLNPMSFPCRLPVSSAVKACEMTGENPGKAPSIVVS
                     VPSFGYSWSEHAAETEKPSQPKGSWWSRLWSRRKSDEEENKITLQTESFEVQIDKTTG
                     GIQGLYPLPYGRNLLGQRLAMRFLQSGTVGDSEDDPEAFYSRMIAETIEQRETVSGTK
                     EVHASGRLVDAGGELVARFTQVIRVCPDLPFIRLDIRLDPERLPEANPWNSYYACRFA
                     WSDESMAVRRSLGFACVETELERFESLYFVQLASSSHSLTLLTPGLPYHRMVGLRKLD
                     TLLLVRGETTREFTVGIGLDIRYPLRAALQLIQPTVAIPSVSPPPSPPSAWLLTLDVQ
                     NVIVSAIEPVVANDQVAELRILLTETEGRAGPLNLRLCRPIQSAYRTDAFGEVLEPLA
                     IKDDQIAANIGRFQTMFISVKFV"
     gene            1507..2181
                     /gene="cmk"
                     /locus_tag="PKI05_09825"
     CDS             1507..2181
                     /gene="cmk"
                     /locus_tag="PKI05_09825"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOA52544.1"
                     /translation="MVVTIDGPAASGKSTVAKMLAQQLGFRYLDTGAMYRAVALAGLR
                     KGTDWDDADQVSRLLDGLDLRITGTKVFLGKEDVTETIRTPEVTRVTSIAADSPLVRQ
                     YLIGMQRAIAKETNLITEGRDQGTVVFPDADCKFFLVASPEERARRRCRDYERLGKPT
                     AYEEVLKDILERDQRDRERPFGGLARAEDAIEINTDNLSPEEVVALIVPIIRAKSEGK
                     RDSQTK"
     gene            complement(2318..>2824)
                     /locus_tag="PKI05_09830"
     CDS             complement(2318..>2824)
                     /locus_tag="PKI05_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008670538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOA52545.1"
                     /translation="EVFEQRNAEVESRGGVPAKAVEPTPARAAVQLLGVSKAAVQSDS
                     FISAASFQETTRVLTEAALASKVDRLVGLKENVILGRLIPAGTGFRKYQRAEVRLRPE
                     AVEKLAAKPATAIAAKFALLEDIPDKETPRATPPDAGNGQNNTVLSVGEGSQDVKSSP
                     SSSSVQQE"
BASE COUNT          591 a          845 c          789 g          599 t
ORIGIN      
        1 tgggacgaaa tcgcaatgca gtcctggcaa cttgcgcaag aggctctgaa acaaagtgac
       61 gacagtctca cggctcatga cgcgatgccg aactcggggg agttgttgtc gcaactttcc
      121 gatgccatcc gtcgtgtggc ctctgccctg gttgagctat taccgcgcag ccgagatgcc
      181 acctcggtcg gcactttggt cctcaacccc atgagcttcc cctgtcgtct ccctgtgagt
      241 tccgcggtca aggcatgcga aatgaccggc gaaaatccgg ggaaggcccc gtcaatcgtg
      301 gtatcggtcc cctcgtttgg gtactcttgg agcgagcacg ccgctgaaac ggaaaagccc
      361 tcccaaccaa aaggctcctg gtggagtcgt ctttggtcac ggagaaagtc tgacgaggaa
      421 gaaaacaaga tcaccctgca aaccgaatct ttcgaggtcc aaatcgacaa aacaaccggt
      481 ggcattcaag ggctgtatcc gctgccttac ggccggaatc tcctcggcca acgcttggcg
      541 atgcgcttcc ttcagagcgg cacagtgggg gactccgaag atgatccgga ggccttttac
      601 tcccgtatga ttgccgagac gatcgagcag agggagaccg tttcggggac gaaagaggta
      661 cacgcatcgg gccgtcttgt cgatgcagga ggcgagcttg tcgcccgatt tacccaagtc
      721 atccgtgtgt gtccggattt gccattcata cgcctcgaca tccgtttaga ccccgagcgg
      781 ctgccggagg ccaacccctg gaattcatat tacgcatgcc gtttcgcctg gagcgacgag
      841 tccatggccg ttcgccgctc cctgggcttt gcgtgtgtgg aaactgaact ggagaggttc
      901 gagagccttt attttgtgca actggcaagt tcgtcccatt cattgacgct attaacaccg
      961 ggtttgcctt accatcgcat ggtcgggctc cgtaaattgg acacgttact acttgtccgc
     1021 ggcgaaacaa cacgagagtt cactgtgggc atcgggctgg atatccggta tccgctccgc
     1081 gcggccctcc aactgattca accgaccgtc gcgattccaa gtgtctctcc gccgccttcc
     1141 ccgccttcgg cctggcttct gacgcttgac gttcaaaacg tcatcgttag cgctatcgag
     1201 ccggtagtcg ccaacgacca ggttgccgag ttgcgaatcc ttcttacaga aacagaggga
     1261 cgggcagggc cgctcaacct tcggctctgt cggcctattc agtccgcgta tcggacagat
     1321 gcctttggcg aggtcctcga accccttgcc atcaaggatg accaaattgc cgcaaacatt
     1381 ggacggtttc agacgatgtt catctccgtg aaatttgtct gagcgcgatc gatagccatg
     1441 caggacccct ttcgagcatc agaaaccaat accccatgca ataggtctgg ggcaaacaca
     1501 ccgttcatgg tcgtgaccat cgacggcccg gccgcctccg ggaagagtac cgtcgccaag
     1561 atgctggccc aacaattggg atttcggtat ctcgacacag gagccatgta ccgcgctgtg
     1621 gccttggccg ggttgcgaaa aggtaccgac tgggacgatg ccgaccaggt ctctcggctt
     1681 ttggatggtc tcgaccttcg gataaccggc acgaaggtct tcctcggcaa ggaagatgtt
     1741 accgaaacca ttcgcacgcc tgaagtcaca cgtgttacct cgatcgcagc cgattcaccg
     1801 ttggtgaggc agtatcttat cggcatgcaa cgggccatcg caaaggaaac aaacctcatc
     1861 accgaaggcc gcgatcaagg taccgtagtc ttccccgatg ccgattgcaa attctttctg
     1921 gtggcatccc ctgaggaacg cgcccgccgt cgttgccgtg attatgagcg actgggtaaa
     1981 ccgaccgcgt atgaggaggt ccttaaggac atcctagaac gagatcagcg cgaccgggag
     2041 cgtccattcg gaggactggc ccgcgccgag gatgccattg agatcaatac cgacaatctt
     2101 tccccggagg aagtggtcgc cctgatcgtt cccatcatcc gggcaaaaag cgagggaaag
     2161 cgcgattcgc aaacgaaata gggcgcagtg gggcacccat gaacgtcggg tccaactgca
     2221 cctcgccgct gatcaatcgt gaattacttc gcgcgtcccc gccgcttcag ggcagaaaca
     2281 cacgccaagc cgtcgacgcg tgttggtcct ccaagcctca ttcttgttgc acggaggatg
     2341 acgacggcga cgacttcaca tcctggcttc cttcgcctac agaaaggaca gtgttattct
     2401 ggccatttcc tgcgtcgggc ggcgtcgctc gcggggtttc cttgtcgggg atgtcctcaa
     2461 gcagggcaaa cttcgcggca atcgctgttg cgggtttcgc cgccagcttt tcgactgcct
     2521 ccggtcgtaa ccgaacctcc gcccgctgat actttcgaaa accggtaccg gcgggaatga
     2581 gtcggcccag gatgacgttt tccttcaacc cgaccaaacg atcaactttg ctggcaaggg
     2641 ctgcttccgt gaggacccgg gttgtttcct ggaagctggc cgccgagata aaactatcac
     2701 tctgcacagc cgccttggaa acgccgagca actgaacggc cgcccgtgct ggggtaggtt
     2761 ccacggcctt ggccgggacg ccaccacgcg attctacttc ggcgttccgc tgttcaaaga
     2821 cttc
//
LOCUS       DAOPTR010000497         2823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_28425_length_2823_cov_3.191113, whole genome shotgun sequence.
ACCESSION   DAOPTR010000497 DAOPTR010000000
VERSION     DAOPTR010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28425_length_2823_cov_3.191113"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PKI05_09835"
     CDS             complement(<1..629)
                     /locus_tag="PKI05_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52546.1"
                     /translation="MIVGEDLPVRDPTYGIAEVHRCLAWLLSEIPQLKAALGELAEPP
                     GWEAMRKRFQSLEFCRNKGLSPPLVAVLFGPSGGGKSTWFRLLTGVNVPAGEEQRPTT
                     CACVVAVPKKLWEREFLEGVFPSCELAELQDPNQLRVSSDRVFFCPQEDPQGLPFILA
                     DVPDFNTVESQNWERAERMLARAELVLFLTYSESYSDRRTVEMLARCCR"
     gene            1384..2094
                     /locus_tag="PKI05_09840"
     CDS             1384..2094
                     /locus_tag="PKI05_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52547.1"
                     /translation="MVPLQHVVCVRGGVARPLLALFFVGLFLINGFLFLIAIAFELAF
                     AILSLAFFTAAGSCLVYYFLRKKFFVLIRSEAGTEIAISFMPSVLEGQTVDFPKALMA
                     VEVLKNLALDALHRTPPTTVAPAFEETPAYPVEDQSQVSISPRVTPPPLTPEAVSPIF
                     TGPEPPPSHEPEPEEVFTPQAPEEVAMKALREAVKTYNDGRHEQGISLLQEVVRLNPG
                     TKAARIAERHLAKLGRHA"
     gene            complement(2183..>2823)
                     /locus_tag="PKI05_09845"
     CDS             complement(2183..>2823)
                     /locus_tag="PKI05_09845"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA52548.1"
                     /translation="IVPVTRVWFNENTSPMWSSVPALLAILTNLVALLVTSLSVARER
                     ELGTFDQLLVSPLRPAEILIGKAVPAFVIAYLEGGLMVGAVIIVFGIPFRGSLVLLAM
                     AMFVFLLAIVGIGLWISSLAATQQQALLGSFTYMVPAVLLSGFATPVENIHPIIRWLS
                     DIDPLRYMVAISRMVFLEDPSLDVIVPYVAPLLPIAAFTLFSAAWLFRHRLE"
BASE COUNT          653 a          797 c          746 g          627 t
ORIGIN      
        1 aaccggcagc accgggccag catttccacc gtccgccgat ccgagtagga ctcactataa
       61 gtgaggaaca agacaagctc ggctcgggca agcatcctct ccgcccgttc ccagttctgc
      121 gactccactg tattgaaatc cggcacatca gccagaataa acggcaatcc ttgaggatcc
      181 tcctgcggac agaaaaacac gcggtcgctg cttacccgca attgattggg gtcttggagc
      241 tctgcgagct cacaggaggg aaaaacacct tccaagaatt ctctttccca gagctttttc
      301 ggtacggcca cgacgcaagc gcaggtggtc ggccgctgtt cttcgcccgc tggaacattc
      361 acccctgtca gaagacgaaa ccaggtgctt tttccacctc cacttggtcc gaaaagcacg
      421 gccacaagcg gcggagatag gcctttgttg cggcagaact ccaaggactg gaaacgtttt
      481 cgcatggcct cccagcctgg tggctcagcg agttccccaa gagcagcttt caattgggga
      541 atttcggaaa gcaaccaagc caggcaccgg tggacctcag cgatgccgta tgttgggtct
      601 cgcaccggca agtcttcccc cacgatcatg agctgaaatc cgtcttcatg ctacatagat
      661 gaaatcggcc agaatgttga cctccggaac tgccccagac acggtttctc tcccattaac
      721 aagccaaatg ggcgactatc gtgattatag gtcgcatcgc atcccccaac aaatggcggg
      781 gctacaacgg ttcttcgcga gaaagatagc caaatcgcaa gttccgtccg gggcgattca
      841 aacctcgctg aagacggtcc tggccatggg aggccaatcc cgcaagacct caaggagccg
      901 gtggtgccaa gttttaggct catgagagcg ccccacccgc gtggggcgcc actatcgtgc
      961 ccattccaaa aggcaaatgt tgacagtcct tctggccctg gttagagtag tgtttggttc
     1021 tagcggcagt tcgtcccaag tcacattttg actttagcgc aaaaggaatg gggtatggct
     1081 tcgcccggcg ggcttgaggt gttcggtgcg ttaatcacct tcgtcaactt cgtcgcagcg
     1141 tgttttttac taggactgat actattcaga agtattgcag ggggctctct cgttttacgc
     1201 tccttcgaat tgcattcgca tcctacctct attcagtcgg atgtcattca tgtggtggga
     1261 cggcgagctg gtctgatttc ttattttcta accctgctag ggctttcctc gacgtgtaca
     1321 tttcgggtaa cccaccaata tgcggagttc gtggattcca gcttgtttgg caagacacat
     1381 gcaatggttc cgctccaaca tgtagtgtgc gtacggggcg gcgtcgcacg gccgctgctt
     1441 gcgctttttt tcgtgggact tttcctcatc aacggcttcc tttttttgat cgcgattgcc
     1501 tttgaattgg cttttgctat tctctccctt gccttcttta cggcagccgg ttcgtgtcta
     1561 gtctattact ttctaagaaa gaaattcttc gtgttgattc gctctgaggc ggggacagag
     1621 atcgctattt cgttcatgcc cagtgtcctc gaaggccaaa cggttgactt tccgaaggcc
     1681 ttaatggcag tggaggtttt aaagaacctt gccctggacg cgcttcatcg aaccccgccc
     1741 acgaccgttg caccggcttt tgaggaaacc cctgcgtacc cggtcgagga tcagtcgcag
     1801 gtttccatat cgccaagggt taccccccct ccgcttaccc cggaagccgt gagccccatc
     1861 tttaccggcc cagaaccacc accgagccat gaacccgagc cggaggaggt ctttacgccc
     1921 caggcccccg aagaggtcgc tatgaaagcc ctacgagagg ccgttaagac ctacaacgat
     1981 ggccgacacg aacagggcat ttccttgctt caggaagtcg ttcgtctcaa tccgggaacc
     2041 aaagctgccc gcattgccga gagacatttg gcaaaactgg ggcgccatgc gtgagcggag
     2101 caccaagatc gttgacttct taccagcatc tgtcatcccg gtgctgttgc acgttactag
     2161 gcagagctgg tcgtggtgtt ctctattcta atcggtgacg gaacaaccat gcggcgctga
     2221 aaagcgtaaa ggctgcaatg ggtaagagag gggcaacata aggaacgatt acatcgaggc
     2281 ttggatcttc gagaaacacc atccggctaa tggccaccat gtagcgaagc gggtcaatgt
     2341 ccgaaagcca ccggatgatc ggatggatgt tttctaccgg cgtggcaaaa ccggaaagaa
     2401 ggacggccgg gaccatgtac gtgaagcttc ctaatagggc ttgttgttgc gttgcagcca
     2461 gcgaagaaat ccacaacccg atgcccacaa tcgcgaggag aaacacaaac atggccatgg
     2521 ccaggagcac gagggagcca cgaaaaggga tgccgaaaac gatgatgaca gcacctacca
     2581 tcaaacctcc ctccaaatag gcaatgacaa acgccggaac ggccttaccg ataagaatct
     2641 ccgccggtcg caaaggtgag accagcagtt ggtcgaacgt ccccagttca cgttcccgcg
     2701 ccaccgagag actcgtcacc agtagtgcca ccagattggt caaaatagcg aggagtgccg
     2761 gaacggacga ccacattggg gacgtatttt cgttgaacca gacccgagtg accgggacaa
     2821 ttt
//
LOCUS       DAOPTR010000498         2820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_28469_length_2820_cov_3.109946, whole genome shotgun sequence.
ACCESSION   DAOPTR010000498 DAOPTR010000000
VERSION     DAOPTR010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28469_length_2820_cov_3.109946"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PKI05_09850"
     CDS             <1..508
                     /locus_tag="PKI05_09850"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOA52549.1"
                     /translation="KHHAAYFRNLNNLVEGKPEAQLSLRELVIQAPAGPVFNNAAQAW
                     NHSFYWDCLSPKGGGEPAGALGAAIARDFGSFAEFRAAMSDAGVKLFGSGWAWLAADK
                     DGKLKILALSNAETPLKLGLEPVLTIDVWEHAYYIDYRHERAKYVEAFWGYVNWEFVA
                     KCYAAATG"
     gene            1404..2246
                     /locus_tag="PKI05_09855"
     CDS             1404..2246
                     /locus_tag="PKI05_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOA52550.1"
                     /translation="MDGKARLQVVKDKDGRVRVVSPPYEIAWERACDDFIRYYNMDDK
                     QSKAARETCERFVTGLQNYLAENQEAILGYLASLKRFEEEEKASPNNGAHFQRERLWE
                     RQQKLRAEVAGWLRQIDAMGEAYQRALWDLLTEEQKAKGMLKVPLCETQRLPVRIPWI
                     ATRSELLNVVVTYSLTAIGLCLILGFFTRLANLGGALFLAFVVMTQWPWPTVVPKTPE
                     IVGHALFVDKNFVEMMAMLALAALPVGRWAGLDYFLYHYVCVPLRQRWGCCCCSKAAE
                     QKKG"
     gene            2326..>2820
                     /locus_tag="PKI05_09860"
     CDS             2326..>2820
                     /locus_tag="PKI05_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA52551.1"
                     /translation="MNLTPEEREIGKANFHRALGNNLEVTRRDFLQSGLLTALAAGGS
                     VGAFYFGYESTIEKPLRVAVLGTGDEGQVLIGACNPEFIDIVAIADIRPFNIHRAFHG
                     DWSSDAALEARPGLMKVYGWKTEDEARKHVKVYGHYEELLKEEKDNIEAVIIALPLHL
                     HAPPA"
BASE COUNT          616 a          653 c          856 g          695 t
ORIGIN      
        1 aaagcatcac gccgcgtact ttaggaacct gaataacctg gtggaaggga aacctgaagc
       61 ccagttgtca cttcgcgaat tggtcatcca agcgccagca gggccggtgt tcaacaatgc
      121 tgcccaagcg tggaaccaca gtttctactg ggattgcctt tcgcctaagg gcggtggcga
      181 gccggctggc gcgcttggtg ctgcgatcgc tcgggacttt ggaagttttg cagaatttcg
      241 cgccgccatg tctgatgcag gggtgaaatt gtttggctcg gggtgggctt ggttggcggc
      301 cgacaaggac ggcaagttga agatcctggc cctcagcaac gccgagactc ccctgaagtt
      361 gggccttgag ccagtcttga ccatcgacgt gtgggagcac gcttactaca tcgattatcg
      421 ccatgagcgg gcgaaatatg tggaggcgtt ttggggctat gtcaactggg aatttgtagc
      481 gaagtgttat gctgccgcga caggctgatt ttgtgacgga ggtcgaactt cgggccctcc
      541 gttggcccga ctttcaaggc tactgataat caccccgtgt tagctggtct ttttgtgcaa
      601 atttgcggcg ccagtccgac tgggggaggt tgcctctttg gacaacctcc cctttatgtg
      661 ttcgtcgcaa tagctagccg aggcgtggag ctaccggctg cggaaatgcg acgggccaac
      721 tcaggttaac ttccggaaga acgtctgatg attttgtatt ggctctggtc cttgtgaatc
      781 ttcggtcccg ttcatttttc ttcggaaagt gcatcggcgg tatcccgcgg tgagtttcat
      841 taggcgtacc accgacggaa caaagaccga tcttgttgcg tagttgaaag tagccacact
      901 tcagaggttg accggccagt tgtggcaaat agccaccaga aggtgctccc aaaaggatct
      961 ggcgatgtgg gccgacggga attggtccgc gccgaggaag ggacgaaaaa acggcccgag
     1021 cctggaacat ttttggcccc gtttggttct ggcaaacacc cgccgttgga attaaaatga
     1081 ccctaacagg tcttgaatag ttggcccact cccgaagtgt tgcgtttgtg gtactgttta
     1141 ccgagcccac cggggatgca tttttggtct tgatcggggt ttgaccgatc gcatacggaa
     1201 ggaggttagc cctgaaccgc tcagctttta cgattggcct ttcggcagtt gtactattgg
     1261 ttttgttacg tatcacgatc ggttggcatt ttctttatga aggggtttgg aaaattgcca
     1321 atcgggatac attctctgcg gagccttttc taacccaggc caaagggccc ttcgccccta
     1381 tttttttcgc aatggtgccc gatttggatg ggaaggcacg cctccaagta gtcaaggaca
     1441 aagatggccg agtccgggtc gtatctcccc cgtatgagat tgcatgggag cgggcgtgtg
     1501 acgattttat tcgttattac aatatggacg acaaacaatc gaaggcagcg cgggaaacgt
     1561 gcgagcgttt cgtgacgggc ctgcagaatt atctggcgga aaatcaagaa gccattttgg
     1621 gctatttagc aagcttgaaa cgatttgagg aagaagagaa ggcctcaccg aataacggtg
     1681 cccattttca acgagagcgg ttatgggaac gtcagcaaaa actgcgtgct gaggtggccg
     1741 gttggctgag acagatcgac gcgatgggag aagcttacca gcgcgctttg tgggatttgc
     1801 tgacggagga acaaaaagcg aaggggatgt taaaggttcc cttgtgtgaa acgcaacgtc
     1861 tgcccgttcg catcccatgg attgcaacga gatcggaact gttgaatgtg gtggttactt
     1921 actcgctgac agcgatcggc ttgtgcctta tcctgggatt cttcactcgg ttggcaaatt
     1981 tggggggcgc gttgtttttg gcctttgtcg tgatgacgca gtggccttgg cccacggtcg
     2041 tcccaaagac tcccgaaatc gtgggccatg cgctctttgt ggataagaat tttgtggaga
     2101 tgatggccat gctggccctt gcagcactgc ccgtaggacg atgggctggt ttagattatt
     2161 tcctgtatca ttacgtatgt gttccgctgc ggcagcgctg ggggtgctgt tgctgcagca
     2221 aggcggcgga acagaaaaag gggtgatcgc gaattgttgg tcattggcgt ctgaaagatg
     2281 tggaagcgat aaacgattgg ctatcagcga taaaggatcg tcacgatgaa cttgacccct
     2341 gaagaacgtg aaattggcaa ggcgaacttt catcgggcat tgggaaacaa tttagaggtc
     2401 acccggcggg atttcctcca gtcagggctc ctaaccgccc tggccgcggg tggaagtgtg
     2461 ggagcgtttt acttcggcta tgaaagcacg attgagaagc cgttgcgggt agctgtgctt
     2521 ggaactgggg acgaaggtca ggtccttatt ggtgcctgta accccgagtt catcgacatt
     2581 gtggccattg cggacattcg tccctttaac attcatcggg ctttccacgg tgactggtcc
     2641 agtgatgcgg ccctggaagc gaggcccggc ctgatgaagg tgtacggatg gaaaacagag
     2701 gacgaggcga gaaagcatgt caaggtctat gggcattatg aagaacttct aaaggaggag
     2761 aaggataaca tcgaggcggt cattattgca ttgccgcttc acctccacgc acctcctgca
//
LOCUS       DAOPTR010000499         2807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_28652_length_2807_cov_3.323765, whole genome shotgun sequence.
ACCESSION   DAOPTR010000499 DAOPTR010000000
VERSION     DAOPTR010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28652_length_2807_cov_3.323765"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            443..1480
                     /gene="tilS"
                     /locus_tag="PKI05_09865"
     CDS             443..1480
                     /gene="tilS"
                     /locus_tag="PKI05_09865"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOA52552.1"
                     /translation="MSKNNIISCLEERFLASFPLHGWAGCRTLVAVSGGPDSVALLHL
                     LLRCGGPREQIVVGHVNHRLRGHDSELDAEFVAELCHRLGVKLVMRAVDPPSGGGDGL
                     EEAARRLRYGCLREFAEQEGTRFVLTAHTADDQAETILYRLFRGTGLHGLRGIPRSRP
                     FGPAALVRPLLNFRRQELLDYLHELGETYREDRTNDSLTFARNRVRHLVIPAALQVHP
                     AAIDGVLKLGEVAQETWGFIQRQVELAIDACVKSRAKNLVVIDRGKLARLDPFLIQGL
                     LVELWRSQQWPMRDLSRKHILAMCRFVTSSPSKPGSDTSIHLPGHIECRVDEENVILR
                     LLTSRCDEHQA"
     gene            complement(2191..2673)
                     /locus_tag="PKI05_09870"
     CDS             complement(2191..2673)
                     /locus_tag="PKI05_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52553.1"
                     /translation="MEEQLAQTPPLKPDECPPFGRMRKISEITNDISAKPGKFPTECP
                     LQEGEFTPRQWEPIVFTWKASGLCHKPLYFEHAAMERYGHNLGPILQYAEAGTHFFLT
                     IPILPYKMGLNPPDECIYPLGYYRPGSCAPWIIDPFPLSVRAGLAEAGLWTGMVFLIP
                     "
BASE COUNT          630 a          755 c          790 g          632 t
ORIGIN      
        1 ggctctaagt gaaccgcttg gccaccagcc ctggggatgc gtaccaccca aagcagggac
       61 aaaaaaccga agaccaacaa ggcagcttca accatacacc agtctcacgg tgcaacagac
      121 tctcgcttgc cgagagcatc gaccaacgaa agcctgtcaa gccatcgcgc ttaacacaaa
      181 cggcttcttc ctctgagttc ccaaatacct cggaatctca ggcctttact acccgtagta
      241 aaatacatca agtccctaaa tcacgattcg gctggttcga ggggttgtta tgcctcgaaa
      301 caagcacgct tacgatatta tgatatacgc ataatgccat agccgcaacc tgagatttcc
      361 gaccaaccgt atcttcattt tccgaaactt ctttcgattt gccagtttcc tgtcgttgtc
      421 tgccaaaagc aatttcgttc tcgtgtcgaa aaacaacata attagctgtc ttgaagagcg
      481 atttctcgcc tctttccccc tacatgggtg ggcggggtgc cggactctgg ttgccgtctc
      541 cggggggccg gatagtgtgg ctcttctcca tctgctcctg cggtgcggcg gaccacgaga
      601 gcagattgtc gtcggccatg ttaaccacag attgcgcgga cacgattcgg aactggatgc
      661 cgaatttgtg gccgaactct gccacagatt gggcgtcaaa ctcgtcatgc gagctgtgga
      721 tccacccagc gggggcgggg atggccttga agaggcggca cggcggcttc gttacggctg
      781 tctacgggaa tttgcagagc aggaaggcac gagatttgtt ctcaccgccc ataccgccga
      841 tgaccaagct gagacgattc tttaccgcct ctttcgtggt acagggttgc acgggttgag
      901 aggtattccg cggagccgcc ccttcggtcc agccgctttg gttcggccgc tcttgaactt
      961 taggcgacag gagctattgg actaccttca cgaactagga gagacttacc gggaagatcg
     1021 aacgaacgac tcgctcacct ttgcccgcaa tcgagtacgc cacttagtga ttccggcggc
     1081 tctccaggta cacccggctg ccattgatgg ggtcctaaaa ctgggcgagg tggctcagga
     1141 aacgtggggt ttcatccaac gtcaagtcga gttagctatt gacgcgtgtg ttaaatccag
     1201 agccaagaat cttgtggtca tcgaccgcgg gaaacttgcc cgcttggacc ccttcctcat
     1261 ccaggggctt ttggtcgaac tctggcggtc gcagcagtgg ccaatgcggg acctgagtcg
     1321 aaaacacatt ttggccatgt gtcgtttcgt gacatcctcc ccctcaaagc cggggagcga
     1381 cacctccatt cacctgccag ggcatattga atgccgagtc gatgaagaga acgtcatcct
     1441 gcgattgttg acctctcgat gtgatgagca tcaagcttga acagcaaggt gatggggctt
     1501 cagtaaaaga cacgctcgcc ccggcccgag ggctataggg gcagagactt cgttcgggtg
     1561 tgctcccttg taacttgcgg agatctttcc gcatcaaaaa acggtacggt aagctataaa
     1621 cctatgtgtt cgagaaacag cacagggcaa gggaaaaaag gccaagccgt ggcacggcgc
     1681 cacagaattc cggctggcct cgagcatttt cgtaatggga aggtccgtgt gccggaggct
     1741 ggctggcgac aaggcgcgat tattggtcag ggctttcgaa ttgccaagac gccaaccgag
     1801 catatatgtt cccgaagacc gggacgccgc tggagtgatt tcccgaacag atgaatgtgg
     1861 cccaaagggt tcgctacaag gagtgaaacg agctatgcca atctacgaat atgaatgtcc
     1921 aaaatgtcat aatcagttcg aatatctcgt ccggggcaat gagccggcga aatgcccgaa
     1981 gtgtggctcg acggagttaa ccaagctctt aagtacgcca tccgtctctg tgaagagtgc
     2041 ggaaagacag cagttgccac cctgcggaac tcctaatccc tgttgtagcg gccagtgtca
     2101 gtttagcgag tgattctcgc ttcgtgatgt cgctatttta cgggcaacgt ccgcgacaaa
     2161 tggcgtctgc gcgaggctaa tggcagcctt tcacgggatg aggaagacca tcccggtcca
     2221 gaggccagct tcggccagcc ctgcccggac gctcagtggg aagggatcga taatccacgg
     2281 agcgcagctc cccggcctgt agtagcctag cggatagata cactcatccg gagggttcag
     2341 acccatcttg tagggcagga taggaatggt caggaagaaa tgtgtgcccg cctcggcata
     2401 ttggaggatg ggccccaggt tatggccgta ccgctccatg gccgcgtgtt cgaaatatag
     2461 cggcttgtgg caaagaccgg aggccttcca agtaaacaca atgggttccc attgacgagg
     2521 ggtaaattct ccctcttgca gcgggcactc tgtagggaac ttgccaggtt tcgccgaaat
     2581 atcgttcgtg atctcgctaa tttttcgcat ccgtccaaaa ggtgggcact cgtcaggttt
     2641 caggggcggg gtttgagcga gttgttcctc catcggtcgg gtgtctaccg gtgcaggggg
     2701 aataccttct aagagttctt gcggagtttt ctgggtctgc cgggtttcag ttcatttcga
     2761 tgcctcgccc ggaccctgtg aaaggacctc cgtcgtgact ggtccac
//
LOCUS       DAOPTR010000500         2790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_28869_length_2790_cov_3.412797, whole genome shotgun sequence.
ACCESSION   DAOPTR010000500 DAOPTR010000000
VERSION     DAOPTR010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28869_length_2790_cov_3.412797"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            793..2667
                     /locus_tag="PKI05_09875"
     CDS             793..2667
                     /locus_tag="PKI05_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52554.1"
                     /translation="MKGSSRDGEVIRLKGRLITIGSAPTCDLRLVAKGIRPLEGFLVR
                     GRVNTVCRAYEGQFTLNGTPTSEGVIRPGDCLGVGPVRLVFIPGGQRQTDAGSEGALP
                     VGPEGVKERVSQMPRFPLRDDVSQLEANAVPDLPTDKELPGDCAERACPVKPEADYLS
                     ANEVREIIESYLREAHLGGTANLAVVQAEVNDLRQAMEETKDAVRKLQTELQSWIEKA
                     AGWDEIAGRIAPTLDETVQETRRQQSVIEELVCQVRAELERLDRMEAEVVRRQIDLAT
                     ERSRLEQTASALAFREQALADSGSVTSWTSESSQMKSPVGLGDDRLYLPGGPEPICDT
                     DSGSSSRSEPAWPEDPIGRETIAYVSEPREPTDTMPPEEGKVVDERLHCNNQAETPPK
                     CDRAVLSLPESGRATTDSDPDHEVVIQDYLARLLGKPVPRPPEKKDTFPKNADAGPPD
                     GGAPGSGEKAEAGGKLPKLAANMPRQRAITRRRVRAPEKNNNLAAMRELANLSSQAAI
                     DRYAKAKLQRLQRGKLAVILVSSLCGLVLVLLDLWVSIGQLGRSAMVLSFIVMVIYSI
                     QYGILTGRLVINSKGQLQLAERRIGREMRKLLPSNKPSLAEAGRDDEAQIEATPERVV
                     "
BASE COUNT          670 a          721 c          808 g          591 t
ORIGIN      
        1 taagtcaagt tgcccaagga ggcctttcct caccggtaca ttccgaaatc accacctcgg
       61 gtcgcttttt gcgcccttac ggaaatccgg ctatcttcgg caaacgtagc gctaaggttc
      121 accaacgcaa atatagtacg taaaccttgc gggttggttt cgtttagcct tttccgactg
      181 gtcgattttc ccttacttgt acccggattg agcaaatttg cagcatttac aggtcgttat
      241 gggctaagtc atgcaaaaac cgtgccgact agcggcaata atcaaggaat cccgataaac
      301 acggttttgc tgtttatccc ggttattctg ctgagcgtta tgggataatc gacaaaacga
      361 aacaatatat ccaggagaaa tgaaaactta aacccgcccc tccgcacgaa tttgacaccg
      421 ccctgggtgc aacctacatc tcattaggat ttctgctggg aagccgccct atctgcggac
      481 accaacctcc atgagacgca gcgaacggcc ctccagtttc aggaaatccg caatttccta
      541 gcctagtgca ccacactgaa taatggctgg ccggaacacg ttgtgacaag gaaatgggag
      601 taggtcccgt tggtaatttc gctttcccct cacaatcgcc cgcagataat cctagtcgcg
      661 ggcggcattt tgtaaatagc cgctaagggt tatccaatac cacacttaag gtcggtaaaa
      721 attagtacac tttgcttgga aaccacgtcg ttggtgagga atgttcagcc cgagcgcgaa
      781 accgagttcg ttgtgaaagg ttcaagtcgc gatggagaag tcataaggct gaaaggcagg
      841 ctcatcacga tcggatccgc cccaacctgc gacttgcgac tcgtggctaa aggcatcagg
      901 cctttggaag gttttttggt cagagggcgc gtcaatacag tgtgtcgagc ttatgaaggg
      961 cagttcacgc tcaacgggac acccacttcg gagggggtta tccgccccgg tgactgtctt
     1021 ggagtcggac cggtgcggct cgtctttatc ccgggagggc aacgccaaac ggatgccggg
     1081 tctgaaggtg ccctgccggt cggtcccgag ggagtaaagg agcgggttag ccaaatgcca
     1141 agatttcctt tacgagatga tgtgtcgcaa cttgaagcaa atgcggtgcc cgatcttccc
     1201 accgacaagg aattgccggg agactgcgcg gagcgtgctt gccccgtcaa gcctgaagcg
     1261 gattatttga gtgcaaatga ggtgcgagaa atcattgaga gctacttgag agaggcccat
     1321 ttaggcggta ctgccaattt ggcagtcgtt caggccgagg tgaacgacct ccgccaggcg
     1381 atggaggaga ccaaggacgc tgtgaggaag cttcagactg aattacagag ctggattgaa
     1441 aaggccgccg gttgggatga aatcgctggg cgaatagccc ccaccttgga tgagacggtt
     1501 caagagacgc gtcgccagca gtcggtgatc gaggagcttg tttgtcaagt tcgggctgag
     1561 cttgaacgac tggatcgcat ggaagccgag gtcgtgcggc ggcaaatcga tctggcgact
     1621 gaacgttcgc ggctggagca gacggcctcg gctttggcct tccgagaaca agcccttgcc
     1681 gattcgggct cggtaacatc gtggacaagt gaatcttccc aaatgaagtc gccggttggt
     1741 ctgggtgacg atcggttgta cctcccaggt ggcccggaac ctatttgcga caccgactct
     1801 ggcagcagta gcaggagtga accggcatgg cctgaagacc ccatcgggag ggagacaatc
     1861 gcttacgtat ccgaaccgcg agaaccgacg gataccatgc caccggagga gggcaaggta
     1921 gttgacgaaa gattgcattg caacaaccaa gcggagacac cacccaagtg tgatagggca
     1981 gttttaagcc tgcccgaatc tggaagggct acgacggatt ctgatccgga ccacgaggtc
     2041 gtcattcaag actatctcgc ccgccttttg gggaaacctg taccgcggcc acctgagaag
     2101 aaagatacct tcccgaaaaa tgccgacgcg ggcccccctg atggtggcgc acccggtagc
     2161 ggtgagaagg cggaggcggg gggaaagttg ccgaagcttg cggccaacat gccgcggcaa
     2221 cgggcgatca caagacgccg tgttcgcgca ccagagaaaa acaacaatct tgccgcgatg
     2281 cgtgaattgg cgaacctgtc atcgcaggcc gcgattgatc gatacgccaa agccaagctg
     2341 caacgccttc agcgcgggaa gctagcagta attctggttt catcgctctg tggtctggtc
     2401 ctcgttctgc tcgatctctg ggtgagcatc ggacaattgg gccggtccgc gatggttctc
     2461 agtttcatcg tcatggtgat atattcgatc caatacggca ttctaacagg ccgtcttgtc
     2521 attaattcca aggggcaact tcaactagcc gaacgtcgga tcgggcggga gatgcgtaaa
     2581 ctgcttccca gcaacaaacc ttctctggcg gaggcgggcc gagacgacga ggcccaaatt
     2641 gaggcgacac ccgaaagagt ggtttagctt cgggtagatt ggctaaaagt tttgtaagcc
     2701 atcgggtggg cgtctggcga cgggttggtt caatactgcg caaggagcac agaagcaact
     2761 cagttgcaca acgcaccgcg ggatatcttg
//
LOCUS       DAOPTR010000501         2787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_28898_length_2787_cov_3.818448, whole genome shotgun sequence.
ACCESSION   DAOPTR010000501 DAOPTR010000000
VERSION     DAOPTR010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28898_length_2787_cov_3.818448"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..1265
                     /gene="galE"
                     /locus_tag="PKI05_09880"
     CDS             276..1265
                     /gene="galE"
                     /locus_tag="PKI05_09880"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009093538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOA52555.1"
                     /translation="MQVLVVGGAGYIGSHAVKMLREQGHTPIVVDNLTRGHRKAVPSD
                     IPFYQISACEIERLCEILKKHSIECVMHFAALAYVGESVEKPLMYYENNVSATIRLLQ
                     AMETVGVQKFVFSSTCATYGEPSTIPITEACPQKPINPYGRSKLMVEQILADYAASHP
                     TFAYAALRYFNVAGASRDGTLGEDHRPETHLIPSILLTLLGRRDKFVVFGTDYPTPDG
                     TCIRDYIHVEDLCAAHILVMEKLCPGQPRIYNLGIGRGYSVKEVIDAVERVTGKRVPV
                     EHGPRRAGDPAILYADATRIRKELNWKPQITTIEEMVETAWRWHSAHPEGYVS"
     gene            1326..2261
                     /locus_tag="PKI05_09885"
     CDS             1326..2261
                     /locus_tag="PKI05_09885"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOA52556.1"
                     /translation="MKATIALVFAVLGSVIVYPVLVYGDEPPKSIEEIVAPGTEPQVI
                     GSGYGFSEGPAADGEGNVYFSDGKNDSIYVYRVGQGVELFVNDSTDANGMMFNSKGEL
                     YVCEGAARRIVAFDVKTKKKRVLASEFQGTAFNEPNDLTIDQQDGFYFTDPNYRHRGQ
                     PSLMKEDVYYVRADGTVSRVSQVCIKPNGILLTPDNKVLYLADNAAGIIYRYDVVGPG
                     QLAHETKWIELGAHPDGMTLDEFGNLYVACGRAGIKVYTKNGEYLGTISVPYASNCVF
                     GGKDFRTLFVTSADKVYAIPTKVRGILPLVLRKQE"
     gene            2432..>2787
                     /locus_tag="PKI05_09890"
     CDS             2432..>2787
                     /locus_tag="PKI05_09890"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HOA52557.1"
                     /translation="MPWLSFFSHIRQWFQSACYFFRKRVLLEDALKILFEQEEAGSQV
                     SPRDLAQTLNVPERCVDRIVEALVERDLARRSGDRIVLTEEGRREALRVVRLHRLWEK
                     YLAERTSLDPSEWHARA"
BASE COUNT          633 a          701 c          795 g          658 t
ORIGIN      
        1 agtcgatccc caaaagtccc cctctccctc ggggagaggg ttagggcgag ggcaatcgat
       61 cgccagagtc gatcgagaaa accggcagcg attcaagcca ccctcacccc cgacccctct
      121 cccgccgacc gggcgagggg agattacggg ataggctcaa agggcgattt gactcttttc
      181 gctaagcgac cgtcgcaaat caacagcaag aaaacaccac gcggaaagga acccgaaaag
      241 gaaaacaccc aaaatagatc tccgggagat tacttatgca ggtcctcgtt gttggcggtg
      301 ctgggtatat tggttcccat gctgtaaaaa tgctgcgtga gcaggggcac accccgattg
      361 tcgttgataa tcttactcgc ggccatcgga aggccgtacc gagcgatatt cccttttacc
      421 agatttccgc ctgtgagatc gaacgtcttt gcgaaattct gaagaagcac tcgattgaat
      481 gtgtgatgca ttttgccgca cttgcctatg ttggcgaatc agtggaaaaa ccgctgatgt
      541 attacgagaa caatgtttcc gcgacgattc ggctcttgca agcgatggag actgtgggcg
      601 ttcagaaatt tgttttcagt tccacctgcg ccacctatgg cgagccaagc accatcccca
      661 tcaccgaggc ttgcccccaa aaacccatta atccgtatgg tcggtccaag ctcatggtgg
      721 aacagattct ggccgactac gccgcgagcc atccaacgtt tgcttatgcc gcgcttcgat
      781 acttcaatgt agcgggggct tcacgtgacg gcactttggg cgaagatcat cgcccggaaa
      841 cgcacctcat cccttcgatt cttttgacgt tgctgggtcg tcgtgacaag ttcgttgtct
      901 ttgggactga ttaccccacg ccggatggaa cgtgcatccg ggattacatt cacgttgaag
      961 acttgtgcgc cgcccatatc ctggtgatgg agaagctttg tcccgggcaa cctcgcattt
     1021 acaatcttgg tatcgggcga gggtactccg tcaaggaggt gattgacgct gtggaacgag
     1081 tgacgggcaa gagagttccc gtcgagcacg gcccgcggcg ggcaggggat ccggcgatcc
     1141 tttacgcgga cgcaacgaga attcgaaaag aattgaactg gaagccccaa atcacaacga
     1201 ttgaagaaat ggtcgaaacc gcttggagat ggcattcggc gcatccggaa ggatatgtga
     1261 gttagttttt ttgcgtcgag gccccatata ctgccgagac gtattcgcaa ctgtggaggt
     1321 gtcatatgaa ggcgactatt gcgttggttt tcgcggtcct gggctcggtc atcgtttatc
     1381 cggtcttggt ttacggtgac gagcccccca agtccatcga agagattgtg gcgcccggaa
     1441 cagaacctca ggttatcggc tctggttacg ggttctccga agggccggca gccgacggtg
     1501 aaggtaatgt ttacttttct gatggcaaaa atgattccat ctacgtgtat cgtgtggggc
     1561 aaggtgtgga actattcgtt aacgatagca ccgacgccaa tggcatgatg ttcaatagca
     1621 aaggcgagct gtacgtctgc gagggagcag cacgtcgcat cgtggccttc gacgtcaaaa
     1681 ccaagaaaaa acgcgtcttg gcaagcgagt tccagggtac ggcgttcaac gagccgaacg
     1741 atctgacgat cgaccaacag gatggattct actttacgga tccgaattat cgtcaccgag
     1801 gtcagccctc cctgatgaag gaggatgtgt attacgtccg tgcagacggg acggtatcgc
     1861 gtgtctcgca ggtgtgtatc aaacccaatg gaatcctgct gacgccggac aacaaggtcc
     1921 tgtatctcgc tgacaatgct gctgggatca tttatcgata tgatgttgtt ggtccggggc
     1981 aattagctca cgaaacaaag tggattgagc tgggtgccca tcctgacggg atgacgctcg
     2041 atgagtttgg caatctgtat gtcgcctgcg gtcgtgccgg aatcaaagtc tataccaaga
     2101 acggtgaata cctgggcacg atatccgttc cgtatgcctc caactgtgtc tttggtggta
     2161 aggacttccg cacccttttt gtgacttcgg cggataaagt ctatgctatt cccacgaagg
     2221 tgaggggaat ccttccgctt gtcctgcgca aacaagagta actgttgtgt gacgatcgta
     2281 ggctatgtcg gaggacgatt ggtcaggtcg gtctctggac ttttttggta atattcaaga
     2341 aggcgacgaa acttttgcga cgggcgtttt cgtctcccac gccactcggt ggggatgctt
     2401 tttcccttga ttgaggctag gtcctacaaa tatgccatgg ttgtcgtttt tttcacacat
     2461 caggcaatgg tttcaatcgg cctgctactt ttttcgcaag cgggtgttgc tggaggacgc
     2521 cctgaaaatc ctttttgagc aagaagaggc aggcagtcag gtcagcccga gagacctagc
     2581 ccaaacgttg aacgtcccgg agcggtgcgt tgaccgcatt gtcgaggcgc ttgtcgaaag
     2641 agaccttgct cggcgttccg gtgaccgtat tgtgctcact gaagagggtc gtcgagaagc
     2701 actgcgggtg gtgcggttac accgactgtg ggaaaagtat ctcgccgaaa ggacgagcct
     2761 cgatccgagc gagtggcatg cccgggc
//
LOCUS       DAOPTR010000502         2782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_28963_length_2782_cov_3.239457, whole genome shotgun sequence.
ACCESSION   DAOPTR010000502 DAOPTR010000000
VERSION     DAOPTR010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28963_length_2782_cov_3.239457"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1861)
                     /locus_tag="PKI05_09895"
     CDS             complement(<1..1861)
                     /locus_tag="PKI05_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HOA52558.1"
                     /translation="MNQQNRRRPPQAYGLYDPQYEHDACGIGAVVNISGKADHRIIDL
                     GKQILLNLMHRGASVADETTGDGAGILFQIPHDFFLEEMASDKIDLPAPGEYGVAMVF
                     FPREDEVRLRCETLLESEVVTSGLTLIGRREVPTDNSCLGEIARACEPRIFQFFIHGN
                     GLRGEELERRLFITRKRLEHRVRGELGEAAADFYIPSMSSLTIVYKGMFIAPQLAAYY
                     PDLSDSRLKSALAIVHQRYSTNTFPSWKLAQPFRMIAHNGEINTLRGNVNHLAAYEKK
                     MTSPLLGQDLSRLCPIIEPGTSDSGAFDNCMELLVRAGRSAPHALMMMIPEAFGPRYH
                     ISTDKRAFYEYHAAIMEPWDGPAAIVFTDGRLVGGTLDRNGLRPCRYVVTDDGLVILA
                     SETGVIEFPPEKIRQKGRLQPGKMFLVDTKEQRIISDNEVKGKIIRQRPYRRWLEQNR
                     IELRGLFLPPTDVESEKGPLEQRLRAFGYTVEDLNMILAPMARDGQEPVGSMGNDAAL
                     PVLSDKPKLLFSYFKQLFAQVTNPPIDPFREGLVMSLMSFTGKHRNLLDETPEHCRQL
                     KLPHPILTNDDIRRLREANHPHLKVATVSILMPAKCENPGATLRETLEKIVDSA"
     gene            complement(2211..>2782)
                     /locus_tag="PKI05_09900"
     CDS             complement(2211..>2782)
                     /locus_tag="PKI05_09900"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOA52559.1"
                     /translation="TEIVVMGAEQLDAYDARTGKQIWYLPGLTGNRTITGPTIENDIV
                     FATIGMRGATFAAKIGGEGRLPPERILWRYEGNNPDSPSPVYSHGLLFLAADNGIVQC
                     LDAKTGELKWRERLGREFKASPLAAEDRIYFLDTSGRCTVVRAAPTFEVLSVNEVKDR
                     SIASPAVSDGHIFIRGRQFVYCIGRPFTR"
BASE COUNT          603 a          790 c          779 g          610 t
ORIGIN      
        1 cggctgagtc cacaatcttc tccagcgttt cgcggagggt tgccccaggg ttttcgcact
       61 tggcgggcat gaggatcgaa acggtggcga ccttgagatg cggatggttg gcctcgcgca
      121 atcgccgaat gtcatcgttc gtcaaaatgg gatgcggcag cttcagttgt cggcagtgct
      181 ccggtgtctc gtccaaaaga ttgcgatgct ttccggtgaa cgacatcaaa gacatcacca
      241 ggccttcgcg gaatggatcg atgggcggat tggtcacctg ggcgaaaagc tgcttgaaat
      301 agctaaacaa caacttgggc ttatcggaaa gcacaggcaa agctgcgtcg ttacccattg
      361 accccacggg ttcctgcccg tcccgtgcca taggcgcgag aatcatgttc aggtcttcta
      421 ccgtgtaccc aaatgcccgc aaacgttgct ccaacggtcc cttctcggat tcaacatcgg
      481 tggggggaag gaagagcccg cgcagttcaa tccggttttg ttccagccag cgccgatagg
      541 gccgttgtcg aatgatcttc cccttgacct cgttgtccga aataatccgc tgttccttcg
      601 tatccaccag gaacatcttg ccgggttgaa ggcggccctt ctgacgaatc ttctccgggg
      661 gaaactcgat gacaccggtc tcgctggcga ggatgaccag tccatcatcc gtgacaacat
      721 aacggcaagg ccgcaagccg ttgcggtcca acgtaccccc taccagtcgg ccatcggtaa
      781 agacgatcgc ggcgggccca tcccaaggct ccatgatggc ggcatgatac tcgtaaaatg
      841 cccgcttgtc ggtgctgatg tgataccgcg ggccgaaggc ctcggggatc atcatcatga
      901 gggcatgtgg cgccgatcgc cctgcccgaa ccaatagctc catgcaatta tcaaacgccc
      961 cagagtcact tgtgcccggc tcgatgatgg gacaaagcct gctcaaatcc tggcccaata
     1021 gcggcgatgt catcttcttt tcataagccg ccagatggtt cacgttgcca cgcagtgtgt
     1081 tgatctcgcc attgtgtgcg atcattcgaa aaggctgcgc caatttccaa ctggggaagg
     1141 tgttggtgct atatcgctga tgaacgatgg ccaaggccga tttcagacga gaatccgaga
     1201 gatccggata atatgcggcc aattgcgggg cgataaacat ccccttatag acgatcgtta
     1261 gggaggacat ggaagggata taaaagtcgg ccgcagcttc cccaagttcg ccccggactc
     1321 gatgttccaa gcgttttctc gtgatgaaca gccgccgctc caattcctct ccccgaagtc
     1381 cgtttccgtg gatgaagaat tgaaagattc gtggctcaca ggcccgggcg atctcgccca
     1441 aacaggaatt gtcggtaggg acttcccgcc gcccgataag cgttagtcca gaggtgacga
     1501 cctcgctttc aagtaatgtt tcgcagcgga gccggacctc gtcttcccgg gggaaaaaga
     1561 ccatagcgac gccgtactca ccaggagccg gcaggtcaat tttgtcggaa gccatttcct
     1621 ccaggaagaa gtcatgcgga atctgaaaga ggatgcccgc cccgtcgccc gtggtttcgt
     1681 ctgccaccga ggccccgcga tgcatcaggt taagcaaaat ctgtttgccg aggtcgatga
     1741 tacgatgatc cgccttaccg gatatgttga ccaccgcacc aatcccacac gcatcgtgct
     1801 cgtattgtgg gtcataaagt ccgtaagcct gtggcggtcg tcttcggttc tgttgattca
     1861 ttgccattca accttgattg cgtagtatcg tcgtttcgtg aattgtattg cgaagcagga
     1921 caaagttaac ctgactttgg cgctgttttg attgtgagag gcttttccct tgtgcgggca
     1981 acggcgtcct ggcagcgtcg gccctaatag gacccagcag tcgcacacgt gatgaggctg
     2041 atcacgtgcc gatcggacgc ctcagcagac gccccattcc tcatcattgt caaggtgcca
     2101 tgagaacttg ccaaggctgc tcttgttatg tgacggggca atttcgctca aggttcgcgg
     2161 caaagagttg ccacaagatc ggctaaaaag cctccgggga gatgaaggag ttaacgagtg
     2221 aaaggtcggc caatgcaata cacgaactgc cgaccgcgaa tgaagatgtg cccatctgac
     2281 acggcgggcg atgcgatgct ccgatccttg acctcgttca cggacaagac ctcgaaggtt
     2341 ggcgccgccc ggacgaccgt gcatcgccca gaggtatcca agaagtagat gcgatcctcg
     2401 gccgccagcg gtgatgcctt aaactcacgt cccagtcgct cacgccactt cagttcgcct
     2461 gtcttggcat cgaggcattg aacgatgccg ttgtccgcgg cgaggaacaa gagcccatgg
     2521 ctgtaaacag gcgaaggact gtcgggatta ttcccttcat agcgccaaag gattctctca
     2581 gggggcaaac gcccctcgcc gccgatcttt gccgcgaagg tggcgccccg catgccgatg
     2641 gtcgcgaaaa cgatatcgtt ctcgatggtt ggacccgtga tcgtccgatt acctgtcagg
     2701 ccggggagat accaaatttg cttgcctgtc cgtgcgtcgt aggcatccaa ttgctcggcc
     2761 cccatcacga cgatctcggt gc
//
LOCUS       DAOPTR010000503         2778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_29009_length_2778_cov_3.939772, whole genome shotgun sequence.
ACCESSION   DAOPTR010000503 DAOPTR010000000
VERSION     DAOPTR010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29009_length_2778_cov_3.939772"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="PKI05_09905"
     CDS             <1..910
                     /locus_tag="PKI05_09905"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOA52560.1"
                     /translation="PVPPAAPPKPAPSSPAPPSPRERLPLPEEVSGTELLTKDGIQLR
                     ATYYPSNRGKDAAVVILLHGWKGSRKDFARIGPFLQQQGYAVFIPDLRGHGESTQRLF
                     PSPGTVTVDKMPPAEIARMVDMDLETCKRFLLQKNNAGELNIEKLCVLGIDATATVAL
                     DWARHDWSWPIYPGLKQGQDVKALILVSPKWSVPGLNAQAALSHPAVSGKLAIYIMVG
                     KDSQKDLADAQRMYNIFARFHPKQDEQSPELQDLFFVKFPTSAQGVELLTRFEQAILQ
                     SIAFFIEQRVAKQPWPWQDRSRKKEG"
     gene            complement(969..1637)
                     /locus_tag="PKI05_09910"
     CDS             complement(969..1637)
                     /locus_tag="PKI05_09910"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOA52561.1"
                     /translation="MDRGFIVFLIVGGLSLGTVWAILSRPRESRSFSHMESLPTRPGP
                     NLAELRSPSSFPEDRLLPSSQELSDAINLTQPPAPSSDLLLSLSPPPAPSVTPFFPKP
                     QTLLKIEQRNVPEKTSKTSGGRLPSGVPSQARNARPGQSFDRLEPAQQVVRHIVADGE
                     TLPIIAQRYYRDITLWTVIYQANQDRLKSPELLPIGTELVIPPIPQDLQSTPFGAEAT
                     RETP"
     gene            complement(1904..>2778)
                     /gene="secF"
                     /locus_tag="PKI05_09915"
     CDS             complement(1904..>2778)
                     /gene="secF"
                     /locus_tag="PKI05_09915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOA52562.1"
                     /translation="DRFAGGTRVLMKFDHKVNYETIESHLKEELAKLPEPDRNAAFEI
                     YNKEYRRGDTTGYENWEIRLALPPEKGLKVVEKVQKRFESTPYFPSSNTIGGKVASST
                     RVRAVYALILSVFGMIVYLWVRFTKVSFGVAAAVALVHDVLVTLGLIALSAYLTGILG
                     FLLIDEFKIGLSVLAAFLTIIGYSVNDTIIIFDRIREVKGKAPYLTPRIVNTSINQTL
                     SRTIITSLTTLFVVVVLYIGGGAGIHAFSFTLLVGVITGTYSSIFIASPLLLWLSPQP
                     VGPASQPQSLQHVS"
BASE COUNT          680 a          745 c          750 g          603 t
ORIGIN      
        1 tcccgttcct cccgcagcac ctcccaaacc ggcaccttca tcccctgccc caccttcgcc
       61 gcgagaacgc ctcccgctac cggaggaggt aagtggaacg gaattgctca caaaggatgg
      121 gattcaactt cgagcgacgt actatccaag caatcggggt aaggatgctg cggtcgtgat
      181 tctacttcac gggtggaagg gaagtcgaaa agattttgcc cgcattgggc cgttcctaca
      241 gcaacaaggg tatgcggtct tcattccaga tttgcgggga cacggagaaa gcacccagcg
      301 gctctttcct tctccgggga cagttacggt tgacaagatg cctcctgctg agattgcccg
      361 tatggtcgat atggatctgg agacgtgcaa gcgattcttg ttacaaaaga acaacgcagg
      421 cgaactaaat attgaaaagc tgtgtgtctt gggcatcgat gcaacagcca cggtggcgct
      481 tgactgggca cggcacgatt ggagctggcc gatctacccc gggttgaaac aggggcaaga
      541 tgtcaaggcc ctgatcttgg tatcgccgaa gtggtctgta ccgggattga acgcccaggc
      601 ggcactgtcg catccggcgg tcagcggcaa gctcgcgatt tacatcatgg tgggtaaaga
      661 ctcacaaaag gatttggctg acgcacagcg gatgtacaat attttcgccc gctttcatcc
      721 caagcaggac gagcaatccc ccgagctgca ggatctgttt tttgtcaagt ttcctacatc
      781 tgcacaagga gttgagttgt tgacccgttt tgagcaagcg atccttcaat cgatagcgtt
      841 cttcatcgag cagcgggtcg ctaagcagcc ctggccctgg caggatcgtt cccggaagaa
      901 ggagggttaa cgttagcaac ggcagagcga acgaggtctg tcacctgccg gtagtgcgca
      961 gtccacggct agggagtttc gcgggttgcc tcggctccga agggagtcga ctgtaaatct
     1021 tgaggaatag gcggaatcac tagttccgtc ccaatcggaa gcaattcggg agattttaac
     1081 cggtcttgat ttgcttgata gatcaccgtc cataaggtga tgtctcgata ataacgttgc
     1141 gcgattatcg gcagtgtctc gccatcggcc acgatatggc gaactacctg ttgggccggt
     1201 tccaacctat caaatgattg acccgggcgg gcattccgcg cttgagaggg cacccccgag
     1261 ggcaggcggc caccggacgt tttggaagtc ttttccggaa cattgcgctg ctcaatcttc
     1321 agaagcgttt gtggcttggg aaagaacggt gtgacggatg gcgccggagg ggggcttagg
     1381 gagagcaata ggtccgagct gggagcagga ggttgtgtta aattgatggc atccgacaac
     1441 tcctgtgagg atgggaggag ccgatcttcg ggaaaagacg agggcgaacg cagttctgcc
     1501 aagtttggac cgggccgcgt cggcaaactt tccatgtgcg aaaaagagcg actttcccgt
     1561 ggtctgctca aaatggccca gaccgttcct aaagagagtc caccgacaat caggaagacg
     1621 ataaaaccac ggtccacgcc gctggtcctg ctttgttgtt tcccgcgagc gcagacatcc
     1681 ttgtcgtgaa cataaaaccc agtccgcaat cctgcaaaac gacccgcgct ttaccctaag
     1741 ttcttatcgg gcaactggca tccgatggca taccccttcc ccaagctcca gattctccaa
     1801 cacattagga gcttccatca ccaccggctc gaagggaaat ccgcaccgat tggcggtgag
     1861 ctcaagcgcg acaaggcgag atctttcagt ttgcccctct cggtcacgac acgtgttgta
     1921 acgattgcgg ctgacttgcg gggcctacgg gttgcggcga taaccacaac aacagtggtg
     1981 aggcaataaa gatggagctg tacgttccgg taatcacgcc gacaagtagg gtgaaactaa
     2041 aggcatggat cccggcacca ccgccgatat acaacaccac caccacaaat agggtcgtca
     2101 acgatgtgat aatcgttcga ctcaaggtct gattgatgct ggtattgaca atccgcggtg
     2161 tcaaatatgg cgccttccct tttacctcgc gaatgcggtc aaagatgata atcgtgtcgt
     2221 tcaccgaata accgatgatc gttaggaaag ctgccagcac agatagacca atcttaaact
     2281 catcgatcag caggaaaccc agaattcccg tgaggtaagc actgagggcg atcaaaccaa
     2341 gcgtaaccaa cacgtcgtgg accaaagcga cggccgctgc caccccgaaa ctgactttgg
     2401 taaaccgaac ccagagatac acaatcatac cgaatacaga aagaatcaag gcatacacgg
     2461 cacggacccg cgtgctgcta gcaactttcc caccaatggt gtttgaagag gggaaataag
     2521 gcgtcgattc aaaccgcttt tgcactttct ccacaacttt tagccccttt tccggaggca
     2581 aggctagacg tatttcccaa ttctcgtagc ccgtcgtatc accacgccgg tactccttgt
     2641 tatagatttc aaaagcggca ttccggtcag gctcaggaag cttggccagt tcctctttca
     2701 aatggctttc aatcgtctcg tagtttacct tatgatcaaa cttcatgagc actcgagtcc
     2761 cgccagcaaa gcgatccg
//
LOCUS       DAOPTR010000504         2771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_29098_length_2771_cov_3.171944, whole genome shotgun sequence.
ACCESSION   DAOPTR010000504 DAOPTR010000000
VERSION     DAOPTR010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29098_length_2771_cov_3.171944"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PKI05_09920"
     CDS             complement(<1..324)
                     /locus_tag="PKI05_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52563.1"
                     /translation="MKKRNVFLVSVGYLMVLAAICGGAVGAESAQPRRLTIVPFTRVH
                     AEDTFWAVRIKTNRERSIPHNLKWCEDTGRISNFAKAAGLMEGKFEGIYFNDSDVYKV
                     LEGASY"
     gene            complement(453..2069)
                     /locus_tag="PKI05_09925"
                     /pseudo
     CDS             complement(453..2069)
                     /locus_tag="PKI05_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650527.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
BASE COUNT          610 a          826 c          697 g          638 t
ORIGIN      
        1 ataggaagcc ccttccagga ctttgtaaac atcagagtcg ttgaaataga ttccttcaaa
       61 cttaccctcc atcaaaccag cggccttggc aaaattggaa atccgccccg tatcctcgca
      121 ccatttcaag ttgtggggga tggatcgctc tcgattggtc ttaatgcgta ccgcccaaaa
      181 cgtgtcttcc gcatggaccc gtgtgaaggg aacgattgtc agccgacgtg gttgtgctga
      241 ctcggcccca acggctcccc cacaaattgc ggcgagtacc atcagataac ccacactaac
      301 gagaaaaacg ttacgttttt tcatttcggc gactctctcc tctgcaatgc cgactttcta
      361 agttgctcgc aaccgaactg actataaaac caatgatcct attacgttcc tcaccgtacg
      421 acctatgtct gatgccatca tttgccgccg tattctagta cctgcccttc gttgagcagt
      481 cgttctcgca gcgcggcata cggcacatcc tgaacagcga tgccctgttt gatggcgagg
      541 tccgctgcgg ctgcggccga ctgaccaagg atcataaaca cgggctccat gcggattgat
      601 ccataagcca catgtgtagc cgagacgcac acgggcacga gcagattctc acagtcttcc
      661 cgcttgggga caagcgagcc gtaggcaatg gcatacggtt tgggagtggg cacaccaatg
      721 tcgccctcgt tctggacgta gccctccggc gtgatgtacc gctgcacgtt gtggctatcg
      781 atggtgtaag aacccattcc caccgagtcg ggtgtcgtct ttttgcctag caggtcgtgt
      841 tccgtcatca cgtacatgcc caccattcgg cgagcttccc gaatgtaaag ctgatagggc
      901 cagtggccat tgtccacaaa ttcgtccttc gcaagtcccc acctgttcat ctcctcttga
      961 acgtctcggg gcacccgcgg atcgttggcg ataaagtaga accagccttt ctgatatgtc
     1021 tcgtgctctt tgataatctc ccgtcgccgc tcataggttg cttcgggata gtcgtaattg
     1081 taaccaatgt tatcggtgct cattggccca tggttattcg tatccgtctt gcggttcggg
     1141 ataggatcga acttatggaa ggtctctcgc cacccggcct cgaaaatccg caacaataat
     1201 tcgtactgtt taggatcgta gccctcgggt ttagggaatg ggacacgatt cgccggcaca
     1261 tccgtcaggc ataatcgaaa acaatatgcc tggacacgat gatcgccctg accgtattcg
     1321 ccgggcggtt ccgtgctgat tcggggtaga accccactgc taggatcacc cggtatccag
     1381 tagggactga taggcttttt tagcactccg aaatgatgac ggtggtgaag aacaccgatt
     1441 tgtactccgg cccacttctc gccgtattcg gcctgcgatt cccgccccac gcgatactga
     1501 cacccggcgg ctgccatcag gtcaccttcg tacgtggcat cgatgaagac ctttgccaca
     1561 aacgtctttc cactgagcat ccgaatactg cggatgcggc tgccctcctt gatcacgcca
     1621 tgctctcgat ccagccattc gtcccggaaa gttggaatct gatactcccg aatgaattcc
     1681 tcaaacacgc cttcagccac gtggggttcg aaaatccaca tggtccgccg ctcgccatcg
     1741 atcgccggcg tcccctgtcc ccgatttccg tattcctcgg gtttttgcca tttccaggct
     1801 tctgggcgtt gatagtgttt ccagatacgg tggtaaaact cccgcgctaa accaccaata
     1861 accgccttgt tgcccgtgtc ggtccaaccc aggccactgc tagacatgcc gcccagatgt
     1921 ttttccggac agacaatggc caccgtccgc ccagctagcc gcgactcgac ggccgccatg
     1981 actccggctg atgtcccacc atacacaacg acatcgtatt cgccagaatg ctcgtctgcc
     2041 gaaagataag cgatgcccaa aatgctcatt acggaaatca atccataaac aattatgttg
     2101 cgaatcatag gaagactccc tgaagctctg ctcatcctcg accgaacatg gtcatgcctt
     2161 gcaatttcgt ccgacactta tccgggcgcg acaatacgaa aattcgtgat ccgccaggcg
     2221 aggcaaactg cttccgtatg ataccaggtt agcgcccgcg tctccacagt ttcgacagcc
     2281 agccgtcgcg gggaattgcg ttaccgctcg ccaagaccgt agtagccttt tcgagccgct
     2341 tgcgttgtat ttttttgagg gcagagtgcg aacctagaaa cgacagaacc caagaaagtt
     2401 atttcggtca ccggagacag taaccctccc gcgctccccc tcccgaggtc accgagggcg
     2461 gtgaccctcc cgatttggtc cccgcggacg gtaaccctcc cggtttggtc actccccggg
     2521 tatgaaccgt gacaagactt ctggccaatg cctgcggaac ttgacaagcg aaatgcccct
     2581 attgaagatg acgattgtgg gttccgtggc cttccggcgg gcatgacaag agcaagaacc
     2641 ttgtgaacag ttacacaaaa tcggcctagg ctgtgacacc agcggggcgg aaggcatgac
     2701 tgaaggtacg cggggcggcg agccgagaga cgaggagggc atcgcaagat ggacgacatc
     2761 gttcgggcgt t
//
LOCUS       DAOPTR010000505         2768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_29135_length_2768_cov_4.166605, whole genome shotgun sequence.
ACCESSION   DAOPTR010000505 DAOPTR010000000
VERSION     DAOPTR010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29135_length_2768_cov_4.166605"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PKI05_09930"
     CDS             <1..583
                     /locus_tag="PKI05_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52564.1"
                     /translation="EVLIAKLADWCRHNDVPLLHAAWYGCDWAELDHGQMVRKARGYS
                     FQVWLDAHRRLMDIALKYSGDDLAIELPFSGHGPLTEAALALADHVISKLGPGNPRFF
                     CQANGWSPRGDWGAPNDDVEAAFDQVWKRPIYRGQQAIQPESFDWSRMYEILRKNRAT
                     YCEVYVRSFVLPGKDELARQIEQFAKSPGVKVE"
     gene            726..2012
                     /locus_tag="PKI05_09935"
     CDS             726..2012
                     /locus_tag="PKI05_09935"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOA52565.1"
                     /translation="MAVTSRERVAMALAHQEPDYVPLDLGGSAVTGMHVSSVYLLRQA
                     LALDPPGTPVKVVEPYQMLGEIDERLQAALGVDVVPLGRRKNMFGFENKDWKPWQLWD
                     GTPVLVPGAFNTTPEPNGDLLMYPEGDKTAPPSGRMPKNGFYFDTIIRQEPIDDEHLN
                     PEDNLEEFTPIDDDELQWLACEADRLYRETDKAILANFGGTAFGDIALVPAPFLKHPK
                     GIRDVAEWYMSTVTRFDYVYRVFERQCEIGIENLKRIYEAVGDRVTAVFITGTDFGQQ
                     HGPFISLKAYRSLYKPFHKRVNDWVHEHTPWKTFIHSCGSVVAFLDDFIEAGFDILNP
                     VQCSAAGMDPATLKQKYGDRLVFWGGGIDTQKTLPFGTPEDVRREVRERMAIFGKGGG
                     FVFNSVHNIQARVPVENLLALFETVNECRGLPFPVR"
     gene            2187..2606
                     /locus_tag="PKI05_09940"
     CDS             2187..2606
                     /locus_tag="PKI05_09940"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOA52566.1"
                     /translation="MTARTRFRLGDLQLKILRILWEKGTASVAEVHASLGKEKFAYTT
                     IATMLRKMENRGLVSHQLENRRFVYFPLVSESQVVRSAASDFVERLFSGSLAAAVCHL
                     LDSCDVTPEELEELEKLIGQRKQTLQEDTKREKRKAN"
BASE COUNT          614 a          686 c          814 g          654 t
ORIGIN      
        1 tgaggtcctc atcgcaaagc tcgcggactg gtgtcgtcac aatgatgtgc cactccttca
       61 tgctgcgtgg tatggttgtg actgggcgga gttggatcac ggccagatgg ttcgcaaggc
      121 ccgggggtat tcctttcagg tgtggctcga tgcccatcgg cgactgatgg atattgcctt
      181 gaaatactcc ggcgacgacc tcgctatcga gctaccgttc tcagggcatg gtccgttgac
      241 agaggcggcg ctcgccttgg ccgaccacgt tatcagtaaa cttggtcccg gcaatcccag
      301 gtttttctgt caggctaacg gatggagccc tcgcggcgat tggggggctc cgaatgatga
      361 cgtggaagct gctttcgacc aggtttggaa aaggccgatt tatcgcggcc agcaggctat
      421 tcaaccagaa agttttgact ggtcccgtat gtacgagatc ttgcgaaaga accgggccac
      481 ttactgcgag gtctatgtga gaagcttcgt tcttccgggt aaggatgagc tggcacggca
      541 aattgaacaa tttgccaagt cacccggcgt gaaggttgag tgaccattat tactcggcgg
      601 ctttccgttc tggtttgtgg cgaaatcacg cgaggccgtg tggagtcgca acgtgggcca
      661 ttcgcgttat aatgctcctt gttggaaaat gagataccgt tgggtcctca tcaaggaggc
      721 attgcatggc tgtgacctct cgtgaacgtg tggcgatggc cctagcgcat caagaaccgg
      781 actatgttcc gctggactta ggcggaagtg ccgtgacggg catgcacgtc agcagtgtct
      841 atctccttcg tcaggccttg gcacttgatc cgcccggcac gccggtcaag gtcgtggaac
      901 catatcaaat gctcggggaa atcgatgagc ggttacaagc agccttaggg gtcgatgtgg
      961 ttcctttggg acgccgaaag aacatgttcg gcttcgaaaa taaggactgg aagccttggc
     1021 agctttggga cggcaccccg gttcttgtac cgggcgcttt caacacgacg ccggagccca
     1081 atggtgacct gcttatgtat cccgagggcg acaagacggc accgcccagt ggacgaatgc
     1141 ctaagaacgg gttttacttc gatacgatca ttcgccagga accgatcgac gacgaacatt
     1201 tgaacccgga agacaacctt gaggagttta ccccgattga cgatgacgag ctccaatggt
     1261 tggcgtgtga ggcggaccgc ctttatcgtg agacggacaa ggctattttg gcgaactttg
     1321 gcggtacggc ctttggcgat attgctcttg tgccagcacc attcctgaaa catcccaagg
     1381 gaatccgtga tgtggcggaa tggtacatga gtacggttac gcgatttgat tatgtctatc
     1441 gcgtttttga aaggcagtgt gaaatcggga tcgagaacct caaacgcatt tacgaagcgg
     1501 tgggggatcg agtaactgcc gtgttcatta cgggcacaga ctttggccag caacatgggc
     1561 cgtttatcag cctcaaagcc taccgatccc tttataaacc gttccataag cgggttaacg
     1621 actgggtaca cgaacatacg ccatggaaaa ccttcatcca ctcatgtggg tcggtggtgg
     1681 catttttgga cgattttatc gaagctgggt tcgatatctt gaatcccgtc cagtgctccg
     1741 cagccggaat ggacccagcg accttgaagc aaaaatatgg tgatcgtttg gttttctggg
     1801 gcggtggcat cgatacgcag aagaccctcc cgtttgggac ccccgaagac gtccggcgag
     1861 aagtgcggga acggatggcc atctttggta aaggcggcgg tttcgtattc aatagcgttc
     1921 acaatattca agcccgcgtc cctgttgaga atctccttgc cctttttgag actgtcaacg
     1981 agtgtcgcgg gctgcctttt cccgtgcgtt agtggacttg ttggtgaata tcggagagtg
     2041 tccaatcgct gcctgcacat ggtcgtttgg acagggtcct ggtcgtgcaa acgcagattc
     2101 gcgaccttcg aggggcttga caagttttcg cgaccgtggt agtattacca tggtagtttt
     2161 caacgccttc aaccgaggaa tggagtatga cagcacgaac cagattccgg ctgggtgacc
     2221 ttcagcttaa gattctgcga atcctttggg aaaaggggac cgcttcggtg gcggaagtgc
     2281 acgccagctt ggggaaggaa aagtttgcct acaccaccat cgccacgatg ctgcgaaaga
     2341 tggagaatcg ggggctggtg tctcaccaac tggagaatcg gagattcgtt tatttcccct
     2401 tggtcagtga atcccaggtc gtccgctcgg cggcatccga ctttgttgag cggctgtttt
     2461 ctggcagttt ggccgcggct gtttgccatc tcctcgatag ctgtgatgtt acaccggagg
     2521 agttagagga gctggaaaaa ctgattggac agcgcaaaca gacgctccaa gaagacacaa
     2581 agcgagaaaa acgcaaagcg aattagattc tatcccaaaa gttctccctc tccctttggg
     2641 agagggttgg ggtgagggaa aaggcacctc ggaatcaatt gagaaaacca gcagcgatcc
     2701 gaggcccccc atcccccacc tctctccggc cgagcaggtg aggggagatt atgagataga
     2761 cccttaat
//
LOCUS       DAOPTR010000506         2764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_29191_length_2764_cov_3.727575, whole genome shotgun sequence.
ACCESSION   DAOPTR010000506 DAOPTR010000000
VERSION     DAOPTR010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29191_length_2764_cov_3.727575"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="PKI05_09945"
     CDS             complement(<1..498)
                     /locus_tag="PKI05_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52567.1"
                     /translation="MRKWVNDRIWWRFGLAAGMLLVVILAADGCVAPATNTAPGAPVL
                     PPADESGGYYREVPGPILAGDCSEFMVTPQETAAAVGSEVILVASVRGQDQFMLTNQR
                     VEWTITPGGVGHFVDFRRGTFMDYLVGDFTRPRKITEHYVITSTSREYLRLTRGTVAP
                     NDDVIV"
     gene            complement(976..1179)
                     /locus_tag="PKI05_09950"
     CDS             complement(976..1179)
                     /locus_tag="PKI05_09950"
                     /inference="COORDINATES: protein motif:HMM:NF039796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HOA52568.1"
                     /translation="MECKKERNLTICTCSYEPCSRKGICCDCLQYHLKNRQLPGCCFP
                     RDAEATYDRSFEHFAKLVQLGRV"
     gene            complement(1493..2383)
                     /gene="xerC"
                     /locus_tag="PKI05_09955"
     CDS             complement(1493..2383)
                     /gene="xerC"
                     /locus_tag="PKI05_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOA52569.1"
                     /translation="MARFLRFLAVEKNASPLTIKSYREDLTALLEYLTEAYQGRCPSP
                     AQITTLDLRGYVAAMHEANYSFATVARRLASLRSFFRFGMREGWVKDNPAKPLRNPRK
                     PRNLPHVLSTEEVRRLLEAPPANDPMGLRDRAILETLYSAGLRISELVGLNDSDLDLR
                     AEVVRVRGKGRRERLAPLGSHAVRAIRRWLAVREIHPSFVDNPDRPLFVNRFKLRLTT
                     RSVARMLEKYLIQTGLDQRTTPHSLRHSFATHLLNAGADIRSVQELLGHKSLVTTQIY
                     THLTTANLKAVYEKAHPRAR"
     gene            complement(2479..>2764)
                     /locus_tag="PKI05_09960"
     CDS             complement(2479..>2764)
                     /locus_tag="PKI05_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52570.1"
                     /translation="LKLAAIDRRRRLIVSREINVIALIKGQERYIFLYDDDNRLEMIR
                     TLGRYAANPELSFSWYDAAVLSQKVRQEARRRALQNRFKAPQPADPEDIF"
BASE COUNT          606 a          782 c          797 g          579 t
ORIGIN      
        1 aacgatgacg tcgtcattcg gggcgaccgt tcctcgagtc agccgcaggt attcgcggga
       61 agtgctggtg atgacgtaat gttcggtgat cttgcggggc cgggtaaaat ccccaacaag
      121 atagtccatg aaggtgccac gtctaaaatc gacaaaatgc ccaacaccgc cgggcgtgat
      181 cgtccactcc acccgctggt ttgtcagcat aaactggtcc tgcccccgca cactcgccac
      241 caggatgact tcgctgccaa cggcggcagc cgtctcctga ggggtcacca tgaattcgct
      301 gcaatccccg gcaaggatgg gcccgggaac ttcgcggtag tatccgccag actcatctgc
      361 cggaggcaag acgggcgcac cgggcgcggt gttggtggca ggcgcaacac aaccgtcggc
      421 tgccaagatc acgacaagaa gcatcccggc agcgaggccg aatctccacc aaatgcggtc
      481 gttaacccat ttcctcatgg gtactgctat ggaatggccg gtctccccaa tccggtcaat
      541 gcattgttaa acctccgggg accgtcacat aaaggacaca aggcacgcgc cagcctcacc
      601 aaggccaccg tcgcggagca ggttgacccc tcatggcaac ggtcaaaggt ccacccatta
      661 gggagaatcg tacccagccc atcccagtga ttacgtcaaa tccccctggc gaatgccaac
      721 cctgccctgg tgacacacac ttttggggat gataagggat tttccaagtt tcttgaagac
      781 cggtttttgt cgctctgccc gaaagggggg atacaggcgg atcgacaatt ggtacgccgg
      841 gggacatgaa ggcgcgtgcc cctccaaatc ccgtggaaca cgtcaggcaa aaaaacgggg
      901 caaccacaag ggttgcctct gtggggatgg cctctctcgc tggttgacaa gaggggatcg
      961 cccttggcga gttacctaaa cgcggccaag ctggaccaat ttggcaaaat gctcaaaaga
     1021 ccggtcatag gtcgcctcgg catcgcgggg gaagcaacat cctggcaatt gccggttctt
     1081 aaggtggtac tgaaggcagt cgcaacatat ccctttccgg gaacaaggct cataggaaca
     1141 ggtacaaatc gtcaggttcc gttctttctt gcattccatc ggaaaactcc ttcttcacca
     1201 ggtcatcgcg ttgctagaca aacggtttcc tcttttctta cgcggctact tgccgatgat
     1261 caaggggctt cccacaccgc gttgtttgga gctcttgtca cgctcattgg caccggaaaa
     1321 gtgacaaatg aaaagaccta ccaccaaaac gttatctccc agcaaggagc gtgcggtaat
     1381 ctctcgcccg gcatgttcgt ccgcaacgcg caaagagcgg cctgcgcttt ataaaggcct
     1441 gggatagtga gtcggcaagc tccgcaaaat tgcccaccgc tagtgatgct gcctatcggg
     1501 cacgaggatg ggccttttca tagacggctt tcaggttggc tgtcgttaga tgcgtataaa
     1561 tctgggtcgt cactaggctt ttgtgaccga ggagctcttg gacgctgcgg atatcggccc
     1621 ctgcgttaag caaatgcgta gcgaagctgt gccgcagtga gtggggcgtg gtccgctgat
     1681 cgaggccggt ttggatgagg tatttttcca gcatccgcgc aacactccgc gttgtcaggc
     1741 gaagcttgaa gcgattgaca aaaaggggtc ggtcaggatt atccacaaac gaggggtgta
     1801 tctcccgtac cgccagccaa cgacggatgg cacgcacagc gtgggagccc agcggggcca
     1861 gccgctccct gcgccctttt cctcggacgc ggaccacttc cgcccgcaaa tcgagatcgg
     1921 aatcgttcag tcccacgagt tcgctaatac gcaggccagc agaatagagg gtttccagga
     1981 tggcgcgatc tcgtaggccc atcggatcgt tggcgggcgg ggcctccaag agtcgccgga
     2041 cctcttctgt cgaaaggacg tggggcaaat tcctcggctt gcgaggattg cggaggggct
     2101 tcgcggggtt gtcttttacc cagccttccc gcatcccaaa gcgaaagaag cttctcagac
     2161 tggcgagacg cctcgcgacg gtggcaaagg agtaattggc ctcgtgcatg gccgccacat
     2221 agccgcgcag gtcaagtgtg gtaatttgag cgggagacgg acaacgccct tggtaggcct
     2281 cggtcaggta ttctagcaga gctgtgaggt cttctcgata actcttgatc gtcaggggcg
     2341 aggcgttttt ctcaaccgca aggaatcgga gaaacctcgc catcgccgtc tgcatcgtca
     2401 actcgcccga tgagccttgc ggtcctctgt aatgatgatt gttcatcgag aaggtacagc
     2461 gtcaatgtct ctgccgggct aaaagatgtc ctccggatcg gcaggttgcg gggccttaaa
     2521 gcggttttgg agggcacgtc gtcgggcctc ttgacgaact ttctgactga gaaccgcggc
     2581 atcgtaccaa ctgaaactca actccggatt ggccgcataa cgtccgagcg tgcggatcat
     2641 ctccagacgg ttatcatcgt cgtaaagaaa gatgtaccgc tcctggcctt tgatcaatgc
     2701 gatgacgtta atttctcggc tcacgatcag gcgtctcctg cggtcaatag ctgcgagctt
     2761 gagt
//
LOCUS       DAOPTR010000507         2763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_29204_length_2763_cov_3.939439, whole genome shotgun sequence.
ACCESSION   DAOPTR010000507 DAOPTR010000000
VERSION     DAOPTR010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29204_length_2763_cov_3.939439"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PKI05_09965"
     CDS             complement(<1..707)
                     /locus_tag="PKI05_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOA52571.1"
                     /translation="MAEATLGADVRQIVLSNAAFGPRDVKALADAMAEDSSQYRALRD
                     AVAELELREETPASNVRLGCCLYLLGHFERAIDVLKRGDNGALAHYYLGRAYIATQKF
                     DLALESFRAARIAGYNSDECALGEVEALRLKGNAAEALQILDRLSGPVVESAEYLFQR
                     GATLACLGASRDEVIELYERAVQSDPYHVGALFGLGLENDRQGNDEVALELYQRCVSR
                     FPPHLGALINLGILYED"
     gene            complement(904..976)
                     /locus_tag="PKI05_09970"
     tRNA            complement(904..976)
                     /locus_tag="PKI05_09970"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(941..943),aa:Thr,seq:cgt)
     gene            1395..2519
                     /locus_tag="PKI05_09975"
     CDS             1395..2519
                     /locus_tag="PKI05_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HOA52572.1"
                     /translation="MSRQSIFEASTRYFLSPILPYLEDDSVSEIMINGPFEVYIERRG
                     KLEKTDARFPSEDALISAINNVAQWVGREIGPEHPVLDARLPDGSRVHAILPPTSRIG
                     TCVTIRKFTKEAMTLEDLVRLNSLSEVVKEFLEICVRIRKNILISGGTGTGKTVLLGA
                     LSRAIPFEERIVVIEDTSELRFVQPHTIYLEARHGGREGQGMTIRQLFSNSLRMRPDR
                     IIVGEVRGGEALDMIQSMLSGHSGSLSTIHASSPRDALIRLETLSLMSDVDIPVYVAR
                     AQVASAIHLVVQIARFTEDGSRKITHVTEVAGLDAENRYHLRDLFVSQLVGRNPDGSL
                     VADLKWTGIQPMFANEPAQQGLLGHVRLSKSLWAPEATDR"
     gene            2580..>2763
                     /locus_tag="PKI05_09980"
     CDS             2580..>2763
                     /locus_tag="PKI05_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA52573.1"
                     /translation="MKLRLGVIGLNDQWEKRYLPALRVLEDRFVLTAIYEPVAQRAAL
                     AARDVGITTHDSFRTLC"
BASE COUNT          604 a          802 c          735 g          622 t
ORIGIN      
        1 tcatcctcat aaaggatgcc aagatttatg agcgctccta ggtgaggcgg aaaacgcgac
       61 acgcaccgct ggtacaattc gagcgctacc tcgtcgttgc cctgtcgatc attttcaaga
      121 cccaaaccga acagggcacc cacatgatac ggatcactct gcacggcccg ttcgtagagc
      181 tcgattactt cgtctcgact cgctcccagg catgccaggg tggcaccccg ttggaaaagg
      241 tactcagcac tttctacgac cggtcccgaa aggcgatcca agatttgtaa ggcctccgca
      301 gcgttgcctt ttaggcggag ggcctcgact tcaccgaggg cgcactcgtc ggaattgtaa
      361 cccgctatgc gggccgctcg aaaactttct aaagccaggt cgaacttctg ggtcgcgata
      421 tatgcccgac caagatagta gtgagccaat gcaccgttat ctcctcgctt gaggacatcg
      481 attgctcgct caaaatgccc gagcagatac aaacagcacc ccaaacggac attgctggcc
      541 ggcgtttctt cccgcaactc gagttccgcc acggcgtccc gcagagcgcg gtactgactg
      601 gaatcttcgg ccatcgcatc ggcgagcgcc ttcacatcgc gcggaccgaa cgccgcatta
      661 ctcaacacga tctgccggac atctgcgccg agtgttgcct cagccattca atcgactccc
      721 tcatcgaaac gatgtaaaga aatgcacacg ggccaattgc aaacgcctca cccgccggtc
      781 tcttgacccc gacaaactat tactgggaac attaacgccc gccgaccggc gtgtgagtgt
      841 tgttcccgcg cgtgatccac ccgaaaaacg ctgccctact cgtcacgaat cagccgtcgc
      901 cagagccaac ggggggactc gaacccccaa cacccgcatt acgaatgcgg tgctctgccg
      961 attgagctac gctggcggca acaagaaacc tccggtttgc cggatcgctc taccatttcc
     1021 attcgtttcc ttaacgatta gcataacaga aagtggactc ttggccaagc ggtccggtta
     1081 ccgctcggaa gggtggttcg cgtctggctc gccaccaatg ggcccttcca gaagccattc
     1141 gttacagggc aacgtcgtcg aggttaattg ttagttccgc acggactttg tgcgaccgat
     1201 ggtagatggt ggcgatagga tgtcacccat aaacctggcg tcgtgtaaac ctggcgtcgt
     1261 ggtgacttcc ccatctttgg gaaatacgtg gcaaaaaggg cttaaatttg tctaaactga
     1321 acgaacacat cgccaaagat tgtattcttt cgctactaga ttaggcagtt ttcggtggct
     1381 acagggtaga agacatgtcc cgccaatcaa tcttcgaggc tagcacccgg tatttcttgt
     1441 cacccatcct cccctatttg gaggatgact ctgtcagcga gatcatgatc aatggcccct
     1501 tcgaagttta catcgagcgt cggggtaagc tcgaaaagac agacgcccga ttcccaagcg
     1561 aggatgccct catttctgcc attaacaatg ttgcccaatg ggttggccgg gagattggcc
     1621 ccgagcaccc tgtcctcgat gcccgcttgc ctgacgggtc acgggtgcac gccatccttc
     1681 cgccgacatc gcggatcggc acctgcgtca cgattcgaaa gtttaccaag gaggcgatga
     1741 cgctcgaaga cctcgtccgc ctgaactcac tctcggaagt agtcaaggaa ttcttggaga
     1801 tttgtgtccg gattcgcaag aatattctga tttccggtgg aacagggacc ggaaagacgg
     1861 tcctcctggg tgccctctcc cgcgccatcc cttttgagga acgaattgtc gtcatcgagg
     1921 atacatccga gcttcgcttc gttcagcccc acacgatcta tttggaagcc cgtcacggag
     1981 gccgagaagg ccaaggaatg acgatccgtc agttgtttag caattctctg cggatgcgtc
     2041 ctgatcggat catcgtggga gaagtccgcg gcggggaagc cctcgatatg atccagtcga
     2101 tgctcagtgg gcattccgga agcctttcta caattcacgc gagttcccct cgggatgccc
     2161 taattcgcct ggagacgtta tcgctcatgt ccgacgtgga tatcccggtt tatgttgccc
     2221 gggcacaggt cgcctcggcc atccatctcg tggtccagat tgcccgtttt accgaggatg
     2281 gttctcgcaa aatcactcat gtgacggagg tggccggtct cgacgctgaa aaccgatatc
     2341 atttgcgaga cctatttgtt tcgcaacttg tgggccgaaa tcccgacggg tcccttgtgg
     2401 ccgatttgaa atggaccggc attcaaccga tgttcgccaa cgagcctgcc cagcagggcc
     2461 tgttggggca tgtccgattg tcaaaatctt tgtgggcacc ggaagcgacc gatcgttgag
     2521 tctcttacgc aggtcacagt ggccgatggt tgtgttggcg ttgcaagaca gggaaagtca
     2581 tgaagctccg tttaggcgtc attggactga atgatcagtg ggaaaaacgg tatctcccgg
     2641 ccttgagggt cctagaggac cgttttgttt tgaccgcaat ctatgagccg gtggcccaaa
     2701 gagcagccct ggctgcgcgc gacgtcggca ttaccacgca cgacagtttt cggaccttat
     2761 gtc
//
LOCUS       DAOPTR010000508         2762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_29217_length_2762_cov_3.412634, whole genome shotgun sequence.
ACCESSION   DAOPTR010000508 DAOPTR010000000
VERSION     DAOPTR010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29217_length_2762_cov_3.412634"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            345..1499
                     /locus_tag="PKI05_09985"
     CDS             345..1499
                     /locus_tag="PKI05_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009095834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HOA52574.1"
                     /translation="MSPFLSIILGLASLGVGGEILVRGAAEIARRLRLSELVIGLTVV
                     AFATSSPELAVSISAAYRGYADVAFGNVVGSNIFNILFVLGLSALVAPLVVNRQLVLR
                     DVPIMIGVSLLTYWFAWNGRIEHHEGFILLALLVGYVALSLILARKSNRNEVSNAVQL
                     LKSDASSESQETEDPPQPRTGILGFFWDVAIFVGLLIVVGIGVAVLMLGAKLFVDGAV
                     LLARFWGVSELVIAVTVVAAGTSLPEAAASIVAGVRGRRDIAIGNVVGSNIFNILCVL
                     GFGTLVTQHGMNVAEEALASDVPIMVGVAVACLPIFITGGVVGRFDGFLLFAYYLAYT
                     ANLVLAVIHSPYLPAFRQVMLWAIIPLTALFLIVSLLHHVVRQRRLGRSG"
     gene            complement(1606..2442)
                     /locus_tag="PKI05_09990"
     CDS             complement(1606..2442)
                     /locus_tag="PKI05_09990"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOA52575.1"
                     /translation="MSRKISFWQKWATKHASGVVVAVVRAWMRTLNYRVVYHDRSADP
                     ISPECRKPHLYLFWHEYILFPLYVREHSYLTMLLSAHRDADILSEVATRMGFYFVRGS
                     TRRGGDRAIRALFAAGRSRHLAITPDGPRGPRRTMAPGAIYLASRLGTPIVAIGFGYD
                     RPWRMNSWDRFAVPRPFSRARAIVSRPIVVPPDLSREGIEEYRTKIQNLLNELTAQAE
                     DWATSGATRADEERVVKAPASLPLHLLPQRIPQIDHVTDGLLLDDNPTLTQLKRATRL
                     AG"
     gene            complement(2574..>2762)
                     /locus_tag="PKI05_09995"
     CDS             complement(2574..>2762)
                     /locus_tag="PKI05_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009565969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOA52576.1"
                     /translation="GETLACGTGACAVCVAGVLAGRLDRKVLVHLLGGDLQIEWRSDG
                     HVFMTGPATEVFTGEWPD"
BASE COUNT          549 a          728 c          794 g          691 t
ORIGIN      
        1 tttcacggtt tttcttggcg agacgcaaga gcggtcgccg gtcgtccata ttcagcacct
       61 aagtcgatct gtggtgatgc tacccttctt ggccacaacg gggtttctta cctggagaca
      121 agaaacatcg ttttcgccag gtgagccgaa gccccccggt gggtttcggc tatcgactcg
      181 gtttagcaat cgagctgtgg aagctaaaat aaaacacttt gattcgaaaa gcttacctga
      241 tacgaatttc actgttgcga caagcatgtc acgaccatta gcagtatttg gccacaggca
      301 gtcggcctaa accaattcag tggacagttc ataggcgaaa catcatgtcc ccatttcttt
      361 caatcatcct tggtctagcg tccctgggag taggtggtga gattctcgtt cgcggggcgg
      421 ccgaaatcgc taggcggttg agattatcgg agttggtcat tggtttaacg gtggtagcat
      481 ttgctacgag ctcaccggaa ttggccgttt ccatcagtgc tgcgtatcgt ggctatgcgg
      541 acgtcgcatt tgggaatgtg gtgggaagta atatcttcaa tattctgttc gtgttgggtt
      601 tatccgccct cgtggccccg ctggtcgtta atcgccagtt ggtgctgcgt gatgtcccca
      661 tcatgattgg cgtttcgctg ctaacttatt ggttcgcatg gaatggccgg atcgaacacc
      721 acgagggttt tatccttctt gcccttctcg taggatacgt cgccctttct ctaatcctag
      781 ctcgcaagag caatcggaat gaagtgtcaa atgccgttca gttgcttaaa agcgatgcgt
      841 ccagcgaaag ccaagagacg gaggacccgc ctcagccgcg aacgggcatt ttgggattct
      901 tctgggatgt ggccatcttt gtcgggttac tcatcgttgt gggcattggt gtggctgtgc
      961 taatgctcgg tgccaagctg tttgtcgatg gtgccgtact gcttgctcgc ttttgggggg
     1021 tgagtgagct tgtcattgcg gtcaccgtgg tcgctgcggg tacatcgcta cccgaagccg
     1081 ccgcctcaat cgtagcgggg gtgcgaggcc gccgcgatat cgccatcgga aatgtggtgg
     1141 gaagcaacat tttcaacatt ctgtgcgtgc taggatttgg aacgttggtg acgcaacatg
     1201 gcatgaatgt ggcggaggag gccctcgctt ctgatgttcc gattatggta ggggtcgcag
     1261 ttgcttgctt acccatcttc ataacgggag gagttgtggg acgtttcgac ggtttcttgt
     1321 tgttcgctta ctacctggcc tatacggcga acctggttct cgcggtgatc cattcgcctt
     1381 atttgccggc ttttcggcag gtcatgctct gggcaatcat cccgctgacg gccctctttt
     1441 tgatcgtgag tcttcttcac cacgttgttc gtcaaaggcg gttgggtcgt tcgggatagc
     1501 agaattgcgg atggaactta ccgaaggcac gcccatcaat tttccaagat tcgcgtacca
     1561 tgagaggggc cgtgacatgt cttcgtgggg accgtcggcg taaaatcacc cggcaagtcg
     1621 cgttgccctt ttcaattggg tgagcgttgg attatcgtcg agaagcaacc cgtcggtcac
     1681 gtgatctatc tgaggaatcc gttggggaag caaatgcagc ggtagcgatg ccggcgcctt
     1741 aacgacgcgt tcttcgtctg cccgggtggc cccggaggtg gcccagtcct cggcctgagc
     1801 agtcaattca ttgaggaggt tctgaatctt ggtgcgatat tcctcgattc cctcgcgcga
     1861 cagatcgggc ggtacaacga ttgggcgact gacaatggcc ctggcacgtg aaaatggcct
     1921 ggggacggca aatcggtccc aactgttcat tcgccatggc cgatcatatc caaacccgat
     1981 tgccacaatc ggcgttccta ggcgagatgc caggtaaata gctcccgggg ccatggttcg
     2041 tcgtggtcct cgcggtccat ccggtgtgat ggccaaatgc cgtgatcgcc cagcggcaaa
     2101 tagtgcccga atagcccggt cacctccccg ccgtgtggag ccgcggacga agtaaaaccc
     2161 catccgggtg gcgacctcgc tgaggatgtc tgcgtcccgg tgtgcggaga gtaacattgt
     2221 gaggtaacta tgttcgcgga cgtaaagagg aaaaagtatg tactcgtgcc agaacaggta
     2281 caagtgaggt ttacggcatt ctggggaaat cggatctgca cttcggtcgt gatacacaac
     2341 tcggtagttg agggttcgca tccatgccct aacgacggcc accacgacac cgctcgcatg
     2401 ctttgtggcc catttttgcc agaaagagat cttcctgctc atctcaactc catccttgag
     2461 gaccgctgga ccctatgaac cgatgttttg caaaactggg tttcactcct ggggtgctca
     2521 atatctggaa ccagtagcta ctccacgagc aacgagatcc agtgccgttg ctcctaatcg
     2581 ggccactcgc cggtgaagac ctccgtggct ggtcccgtca tgaagacgtg accgtcggat
     2641 cgccattcga tctggaggtc tccgccgagc aaatggacca agaccttacg atctaaccga
     2701 cctgcgagga cccctgcgac acaaaccgcg cacgccccgg tgccgcaggc cagcgtctct
     2761 cc
//
LOCUS       DAOPTR010000509         2761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_29228_length_2761_cov_3.301922, whole genome shotgun sequence.
ACCESSION   DAOPTR010000509 DAOPTR010000000
VERSION     DAOPTR010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29228_length_2761_cov_3.301922"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PKI05_10000"
     CDS             complement(<1..414)
                     /locus_tag="PKI05_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52577.1"
                     /translation="MRKEITVSVTSVMLVVGLIAFCLGVGAGWYGAWYQYVKRPMVMQ
                     QQENEKQQQEMEKMTRRGEVRKVSGNSITVNVTAGGADVGKTLTYAVTPTTRVQVGSQ
                     IIGGGDIGGSPVDVTKYISPGDFVHLMVKDGQIVSM"
     gene            complement(435..920)
                     /locus_tag="PKI05_10005"
     CDS             complement(435..920)
                     /locus_tag="PKI05_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52578.1"
                     /translation="MIRKLSPRDQKILVVALAALIFASGYYISWWTNVKVPRDQRAAI
                     AREDLDIKDRTRRGTVEEVSPGKITIKLTFASDRRLIGKTETYDVNYLTNVMVGPDFV
                     NEPGVDPDIVKWFVPGDYVEIIQSKKEPTRAVVLRRELRDGERPVYSLTDPMNPATPQ
                     Y"
     gene            complement(895..1254)
                     /locus_tag="PKI05_10010"
     CDS             complement(895..1254)
                     /locus_tag="PKI05_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52579.1"
                     /translation="MGIGAGIALTGVIVGLIYRRHYLKMTASRVLLLATPEPNKSSLT
                     DLRAVVESVESLIKEYENRTTNEIERFEIQTAQLSALLAEAQSNEERLAAALNQMQKI
                     LGLKEGSEAVDSEVKSA"
     gene            complement(1943..2422)
                     /locus_tag="PKI05_10015"
     CDS             complement(1943..2422)
                     /locus_tag="PKI05_10015"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HOA52580.1"
                     /translation="MLNQRGSSSAVALVVILPLLMVVCLGLSWLGHMAAVKSVCEEAA
                     RAGARQLAVCPDGDLATEILAAPREVMMSSSDINTSASFTVDVADGVRSTGGLPVRID
                     GYAYCRVVYYYPVPIPSFWKKMSQAATPIWGGAGTSAQTTVPIVGEAFFVSEREGVD"
     gene            complement(2415..>2761)
                     /locus_tag="PKI05_10020"
     CDS             complement(2415..>2761)
                     /locus_tag="PKI05_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52581.1"
                     /translation="RGEEKFDFIFVDVFDGLYPPDIFMQEQTIRAISECLNLDGALCM
                     NTVRRHAWDKKHRELRSAFASTFKHTGEAVSGEGRTLPVLPHNVVLVGTNATGVVRRP
                     FSEIPKGVRDHA"
BASE COUNT          588 a          841 c          642 g          690 t
ORIGIN      
        1 catgctgact atttgcccat ccttcaccat caaatgcacg aaatcaccag ggcttatgta
       61 cttagtaaca tcgaccggag atccgccgat atccccgccc ccaatgatct gtgagccgac
      121 ctgaaccctg gtcgtcggtg tcacggcata cgttaaagtc ttgccgacat ccgcaccccc
      181 cgccgttaca ttaacggtta tcgagttccc ggagaccttc ctaacctcgc cccggcgggt
      241 cattttctcc atttcttgtt gttgcttttc attttcttgt tgttgcatga ccataggccg
      301 ttttacatac tgataccatg caccgtacca ccccgcgccg acccccagac agaacgctat
      361 gaggccgacg accagcatta ctgacgtaac tgataccgta atttcttttc tcaagatatc
      421 attcctttcc tttgttagta ttgtggcgtc gccggattca tcgggtcggt caagctgtac
      481 accggcctct cgccgtccct gagttcccgg cgcaacacga ccgcacgggt cggttctttt
      541 ttgctctgga tgatctcgac gtaatcaccg ggtacaaacc atttcactat atccgggtcc
      601 acaccgggtt cgttgacgaa atccggcccg accatgacgt tggttaaata gttaacgtcg
      661 taggtctccg tcttgccgat taaccgccga tccgatgcga atgtgagttt aatcgttatc
      721 ttgcccggcg atacctcttc caccgtgccc cgccgggtcc tgtctttaat gtccagatct
      781 tccctagcta tcgcggcccg ttggtcgcgg ggaaccttga cgttggtcca ccaggatata
      841 tagtagcctg atgcgaagat tagagcggcc aaggcaacaa cgagaatctt ttgatcacgc
      901 ggacttaact tccgaatcaa ccgcctcgct cccctcttta agtcccagga ttttttgcat
      961 ctgatttaag gccgcagcaa gccgttcctc gttgctttgg gcctcggcta acagtgccga
     1021 caattgcgct gtctggatct caaaccgttc gatttcattt gtggtccggt tctcgtattc
     1081 cttgatcaac gactcgacgc tctcaacaac cgcacgcaag tccgttagcg aagatttgtt
     1141 gggttccggg gtcgccagca agagaacccg gcttgcggtc attttgaggt agtgccgccg
     1201 atagatcaag ccgacgataa ccccggtaag agcaatcccc gcccctattc ccaatgcaac
     1261 atccctggtt agcgttgagt ttgtcgaggc cagaactatt gttgtcgcgc acagtatagg
     1321 ccagacgcca attagaaaga taaaccacga tgtcgccccg gcttgttcct gcatgaatga
     1381 ttccggggtc gcggcgggat acctgctttt cctgtttacc attagatctt tctcctttcg
     1441 tttcgttgat cacgggacat cctcaatttc atgtctcgat gcccctgaca cggtatagct
     1501 gaaatgataa ggccactcag ttgaccctct tattaatttc atcaatgtcg gaaaagggtt
     1561 cgctacgtag catgtcaacg tacactcgac ggtttcgccc cggtctatta gagaagttac
     1621 ctcaaccccg ttctgtcccc tggggggata atggccttga ggcaaaaaat ttgaattggg
     1681 tttcatcaat cccccgttca ccaggacctg ttttgccctg gcggtcgctt cctgcccggc
     1741 ctggggattt tttcttccta gttcgatccc atagacccgt gcggcatctc gggcggccat
     1801 cgcgccgatt gccttttgcc ggtccaggtt tagccatgtg cttgcgagca acaggaagaa
     1861 caacaatatt gggatcacga ccaaccacgc gacaacctct atagatccgc gctcgttttc
     1921 caacacctct aataacacgc aattaatcca ctccttctct ctcggaaaca aaaaacgcct
     1981 cacccacaat cggaactgtc gtttgggccg atgtacccgc gccgccccag attggggtcg
     2041 ccgcttggct catctttttc cagaaagacg ggatcggcac gggatagtag tacactaccc
     2101 gacaatacgc atacccgtcg atccgcaccg gcaatccccc tgtggagcgc actccgtcgg
     2161 caacgtccac ggtaaatgat gcggaagtgt taatgtcgct tgacgacatc attacttcgc
     2221 ggggcgcggc aaggatctcg gttgccaggt ctccgtcagg acaaaccgct aattgcctcg
     2281 ccccggcccg cgccgcctct tcgcaaacgc ttttcacggc ggccatatgc cccaaccacg
     2341 acaatcccaa gcatacgacc atgagcaaag ggaggattac gaccaacgcc acggcggacg
     2401 acgagcctct ttggttaagc atggtccctc accccctttg gtatttcgct gaatggccgt
     2461 cgcacaacac ccgtcgcatt tgtaccgacc aacaccacgt tatgaggaag aaccggcaat
     2521 gtccgcccct cgccgctcac ggcctcccct gtatgtttga atgtcgacgc gaatgcggag
     2581 cgcaattccc tgtgcttctt atcccatgcg tggcggcgca cggtgttcat gcacaatgcc
     2641 ccgtcgagat tcaaacactc ggatattgcc cggattgttt gttcctgcat gaatatgtcc
     2701 ggcgggtaca acccgtcgaa cacgtcaacg aaaatgaaat cgaacttttc ctcgcctctg
     2761 a
//
LOCUS       DAOPTR010000510         2752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_29336_length_2752_cov_4.241379, whole genome shotgun sequence.
ACCESSION   DAOPTR010000510 DAOPTR010000000
VERSION     DAOPTR010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29336_length_2752_cov_4.241379"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PKI05_10025"
     CDS             complement(<1..608)
                     /locus_tag="PKI05_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA52582.1"
                     /translation="MNDEYFEGVNSPENQPLPSMQVGSSPENVQESGATEGEINICAL
                     QGQQEYTADKLEHLSDQEHVRKRPSMYIGDTGSRGLHHLVFEVVDNSIDEAMAGYATQ
                     LWVTINPDGSVTVEDNGRGIPVDVHPALGISTLEGVMTLLKFGGKFSKGAYYTSGGLH
                     GVGVTVVNFLSEWCVVEVYRGGAGWRQEYERGKPLGPVRRMG"
     gene            complement(683..985)
                     /locus_tag="PKI05_10030"
     CDS             complement(683..985)
                     /locus_tag="PKI05_10030"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOA52583.1"
                     /translation="MPREPHHISQVVADLLARTGVAQPQATRELEAAWREIVGGVLAD
                     VSRPVRIYRARLEVVVAHSTALQELTFRKGEILQQLNQRWPDRRVKDIKFRVGLLK"
     gene            complement(1033..2142)
                     /gene="dnaN"
                     /locus_tag="PKI05_10035"
     CDS             complement(1033..2142)
                     /gene="dnaN"
                     /locus_tag="PKI05_10035"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOA52584.1"
                     /translation="MKVVADREKLAHVVQLAGAICPGRGTRPILENLFLDCDDHTGTI
                     MATDLEVGIRAEVPDLTVEVPGKTLLPFRLTASVLREINEDQIRLEADGQKVLLLSSR
                     AEFQFPTPDPLEYPLVPAFQGKAYHSFTARTLREMLVRTEFAVELDSTRFNLGGVFLE
                     LAENSAIAVATDGRRLARQEGPAKQVGKHMPTDHVIIPARAVQLIQRIIGDGEEEVKV
                     AIEEGKIFVITGSSSFYARLIDARFPKWREVFPKDSARISVELPVGELAAALRQAAIV
                     VDSKHPGVWTTFTSGKLVLATRGSEYGESRVEVPISYDGEELTLRLDPRFMLEFLKVL
                     DNDATFTWKVWQPETPVLAETTDGYAYIVMPLISS"
     gene            complement(2448..>2752)
                     /locus_tag="PKI05_10040"
     CDS             complement(2448..>2752)
                     /locus_tag="PKI05_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOA52585.1"
                     /translation="PGDEECYTAGPLTIDRSRHEVRCNDQTIALTPTEFAILDLLASH
                     PGRVLSRAEIAESVHGEPFVTTDRAVDVHIAALRRKLGSHGQLIETVRGIGYRFRD"
BASE COUNT          608 a          871 c          701 g          572 t
ORIGIN      
        1 ttacccattc tgcgaacggg gcccaagggc ttgccacgtt cgtattcctg tcgccagccg
       61 gcccccccgc gataaacctc gacgacacac cactcggaca agaaattgac gaccgtcacg
      121 ccgactccgt gcagtccccc tgaggtatag tacgcgccct tgctgaactt gccgccgaat
      181 ttgagaagcg tcatcacacc ttccagcgtg gaaatgccga gggcaggatg cacatcgacg
      241 gggattcctc ggccgttatc ttcgaccgtc accgaaccgt ctgggttgat ggtgacccaa
      301 agctgcgtcg cgtacccggc catggcctcg tcaatggagt tatccacgac ttcaaagacg
      361 aggtgatgga ggccgcgcga accggtgtcc ccaatataca tgctgggacg ctttcgcacg
      421 tgctcttggt cgctaaggtg ttccagtttg tcggcggtat attcctgctg cccctgaaga
      481 gcacagatat tgatctcccc ctcggttgcc ccactttctt gcacgttttc cggtgatgag
      541 ccaacctgca tcgatggcag cggttgattc tccggactat tcacaccttc gaaatactcg
      601 tcattcattg tcacaactcg attgttttct caacgctctc catccgtgcc gaggccgaca
      661 aagaccccgg actcattctc cgtcacttca acagcccgac acgaaacttg atgtctttca
      721 ctcgtcggtc aggccatcgt tggttcagtt gctgcaaaat ctcgcctttg cgaaaggtca
      781 attcctggag ggctgtggaa tgggctacca caacctccag ccgtgcccgg taaatccgca
      841 cgggacgcga cacgtcggca aggacgcctc ccacaatctc ccgccatgct gcctcgagtt
      901 ccctcgtagc ctgaggctgc gccacacccg tccttgccag caagtcagcc actacctggc
      961 taatatggtg tggttcacgc ggcacagcgt cactcctttt cccagcgtcc tcaacccgac
     1021 catcccagct cactacgagg aaatcaacgg catcacgatg taagcgtaac cgtccgttgt
     1081 ctcggctagc acgggagtct ccggttgcca aaccttccat gtgaacgtgg catcgttatc
     1141 gagcaccttc aggaactcaa gcatgaaacg tgggtccaac cgcagcgtga gctcctctcc
     1201 gtcgtaacta atgggcacct ccacccggga ttcaccatac tctgaaccgc gggtcgctag
     1261 aacgagctta ccgctggtga aggtagtcca gacacctggg tgtttggaat ctacgacgat
     1321 tgccgcctga cgcagagccg ccgccaactc gcccaccggc aactccacag atatccttgc
     1381 cgagtccttg ggaaagacct cccgccactt gggaaaacgg gcatcaatga gccgtgcgta
     1441 aaagctcgac gaccctgtaa tgacgaaaat ctttccttct tcaatagcaa ccttgacctc
     1501 ttcttcgcca tcgccgatga tccgctggat aagttgcaca gccctcgccg ggatgatgac
     1561 gtggtcagtg ggcatatgct tccctacctg tttggccggt ccctcttgcc tggccaggcg
     1621 acgcccatcg gtcgccactg cgatggcact gttctccgcc agttctaaaa agaccccgcc
     1681 tagattgaat cgcgtgctgt caagctccac agcaaactcg gtccgcacca acatctcgcg
     1741 caaagtccgg gccgtgaacg agtggtatgc cttgccttga aatgcaggca ccagaggata
     1801 ttcgagcgga tcaggcgtgg ggaactgaaa ctcagcgcga gaactcaata acaacacttt
     1861 ctgtccgtcg gcttccaacc gaatttgatc ttcgttaatt tctcgaagga cggatgcggt
     1921 caaacggaaa ggaagcagcg tcttgccggg cacctcgacc gtcaggtcgg gaacctccgc
     1981 ccgaattccc acctccaggt ctgttgccat gatggtgccc gtgtgatcat cgcaatcgag
     2041 gaagaggttc tccaaaatcg ggcgagtacc ccggccagga caaatcgccc cagccaattg
     2101 aacaacgtgg gcaagcttct cacgatcagc gacgaccttc atggctgcct cgattccgaa
     2161 aaatgcgtcc gaaaacggtg ctatgtcatt gggaattgcg ggtctcaagg aaccagaaaa
     2221 cattatccca caaaagcccc aaatcctcga gtctccccct tagcgaggta ctggtttgaa
     2281 cccatggatg ccccgccgtt agtgggggac tgaatgcgac ttcaatggga caaagcgtac
     2341 ccgcaagagc acgtagctgg tacccgacgc gcccccgtcg caccgcattc gcagcttgtg
     2401 agcgtcgtcc tagagatggc tgccactaag accgggcggt caaattctca gtcacggaag
     2461 cggtacccaa tcccgcgaac tgtctcaatg agctgcccat gcgatccgag ctttcgacgc
     2521 aaagccgcga tatggacatc gacggcccgg tcggtcgtca caaacggttc cccgtggacc
     2581 gactcggcaa tctccgcccg cgagaggact cgtcccggat gcgaagccaa aaggtcaaga
     2641 atagcaaact cggtgggcgt caaagcaatt gtttgatcat tgcaccgcac ctcgtggcgg
     2701 gaacgatcaa tcgttaaagg ccccgccgtg taacattcct cgtcaccagg cc
//
LOCUS       DAOPTR010000511         2751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_29350_length_2751_cov_3.088279, whole genome shotgun sequence.
ACCESSION   DAOPTR010000511 DAOPTR010000000
VERSION     DAOPTR010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29350_length_2751_cov_3.088279"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..680)
                     /gene="ybeY"
                     /locus_tag="PKI05_10045"
     CDS             complement(234..680)
                     /gene="ybeY"
                     /locus_tag="PKI05_10045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOA52586.1"
                     /translation="MYRIAISKQVKSCKINRNLIRKAVRVVLQQAGIETAEISIAIVD
                     DATIAQLHGQYLGDPTPTDVLSFVLEEGDHHLEGEIVASAETAAAWAKRLAWPCENEL
                     LLYIVHGTLHLVGFDDQTPAARRKMRREEKAVLHKLGVIPPVRRRR"
     gene            complement(736..>2751)
                     /locus_tag="PKI05_10050"
     CDS             complement(736..>2751)
                     /locus_tag="PKI05_10050"
                     /inference="COORDINATES: protein motif:HMM:NF015655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HOA52587.1"
                     /translation="GIRDIEFVIQFLQLLHGADLPGVRTGNTLDAIAGLEQAGCLTHT
                     ESAYLQESYHFLRKLEHRLQIMFDLQTHTIPQGAEEIQKLAVRMGYHDTPTRSASEAF
                     EADYYRFTFRNRQIINHLLHDAFRDETETSPEVELVLDPEPSPDFIKQVLSRHRFSNP
                     EQAYRNLMSLAEERVPFLSTRRCRHFLASIAPMLLSAIATTPDPDATLVTLDQVSDSL
                     GGKGVLWELFSFNPPSLKLYVQLCAYTPFLASLLISNPGMIDSLMDSLVLDRLPSREF
                     MENSLRELCLAAEDIDPILHAFKNDHLLQIGVRDLLGKEDVEAITRTISELAETCLME
                     IVVREYEHLVLRYGQPTIQEGPDQGRRAEMVILALGKLGGQEMNYQSDLDLVFLYEGD
                     GQTVPVVDSGYVRRGEVTTNQHFFSELTQRIIRRASYLGPHGRLYQVDARLRPTGRSG
                     LLVFTLNEFRQYFESGQGQLWERQALCKARPVVVTPRMQPVINQLLHEVIHCQPWRPE
                     FAHEIAAMRKRLEATAKGLDIKRGCGGTVDVEFLVQMLQLRHGREFPQLHTTSTMAAL
                     RMLRECGVLEERDFKELFESYRYLRTLENRMQLVAPTARSRLPDNPVELSRLSHLMGA
                     ETPPAMIRTVEAYLARNRSHFERVVEKLANERIALPSSPNQETPHDN"
BASE COUNT          601 a          743 c          797 g          610 t
ORIGIN      
        1 cacttcccga aatcgcccca tgcggttgcg tttacggcag agctcctcta ggtcatggct
       61 ggggaaatcg gaaagggcac gtgttcctgt agaagcaatg cacgatatgc ccagtcccaa
      121 aacacacagc cataaaagca cttggtcctc cgacacgtca ggtagttcct catcgttaac
      181 ggaattgtga cccaaccacg gagcttggtt gccggttatc gcatggcaaa agactaacgg
      241 cgtctgcgaa ccggcggaat aacacctaac ttgtgaagaa ctgctttctc ctcgcggcgc
      301 atttttcttc gtgccgccgg ggtttggtca tcgaagccta caagatgcag tgtaccgtgc
      361 acaatgtaga gcaacaactc gttttcgcaa ggccaggcga gacgttttgc ccaggcagcc
      421 gccgtttctg cgctggcgac gatctcgccc tccaagtggt ggtcgccttc ttccaggaca
      481 aaacttaaca catcggtagg ggtcgggtct cccaggtatt gcccatgaag ctgggcaatc
      541 gtggcgtcat ccacaatggc aatactgatt tcggcggttt cgatgcccgc ctgttgaagg
      601 acgacccgca cggccttccg gatcaagttg cggttgatct tgcatgattt gacctgcttg
      661 ctaatagcaa tgcgatacaa ggtttaacct cccaaatcac aacaacacac aacgttggcc
      721 agcgttctgg cagctttagt tatcatgcgg tgtttcttgg ttgggcgatg aggggagggc
      781 gatccgctcg ttggcaagct tttccacgac acgttcgaag tgggaccgat tacgggcgag
      841 ataagcctcc acggtgcgga tcatggcagg gggtgtttcg gcccccatga ggtggctgag
      901 ccgagaaagt tccacggggt tatcgggcaa gcggctacga gcggtgggcg ccaccagttg
      961 catgcggttc tcaagtgttc ggagataccg gtaactttcg aacaattcct tgaagtctct
     1021 ttcctcaagc acgccgcatt cccgcaacat ccgcaaggcg gccatggtcg atgttgtgtg
     1081 cagttgggga aattcccttc cgtgacgcag ttgcagcatt tgaaccaaaa actcaacatc
     1141 aacagtccct ccgcaaccgc gtttgatatc taacccttta gccgtggctt ccaatcgctt
     1201 gcgcatggct gcgatctcgt gggcgaattc cggtcgccag ggctggcagt gaatgacttc
     1261 gtgcaagagt tggttgatga cgggctgcat ccggggtgtg accacaaccg gacgggcctt
     1321 acatagggcc tgccgttccc acaattgacc ctgaccactt tcaaagtact gccggaactc
     1381 gttgagggtg aataccagca agccgctccg tcccgtgggg cgaagccgtg cgtccacctg
     1441 gtaaagtctg ccatgaggac cgaggtagga agcccgacgt atgatccttt gcgtcaactc
     1501 gctaaagaaa tgttggttgg tggtcacttc ccctcgccgg acgtagcccg aatcgacaac
     1561 aggcacggtc tgcccatcgc cctcgtaaag aaacacgaga tccagatcgc tttgatagtt
     1621 catctcctga ccgcccagct ttccgagggc gaggattacc atctccgcgc ggcgtccttg
     1681 atcggggcct tcttggatgg tgggctggcc ataccgcagc accaggtgtt cgtattcccg
     1741 gacaacaatc tccatgagac aagtttcggc caattcgctg atggtccgcg taatggcctc
     1801 gacgtcctcc ttgccaagaa ggtcacgcac gccaatctgg aggaggtggt cattcttgaa
     1861 ggcgtgcaag atgggatcga tgtcttctgc ggctaggcat agctcacgaa gggagttttc
     1921 cataaactcc cggcttggca gtcggtccaa gacaaggctg tccattaggc tgtcgatcat
     1981 ccccggattg ctgatgagga gactggccaa gaatggtgtg taagcacaca actggacgta
     2041 caacttaagc gacggcggat tgaaactaaa aagctcccac agaacgcctt ttccaccgag
     2101 ggaatcgctg acttggtcaa gcgtcaccaa agtggcgtca ggatcggggg ttgtggcgat
     2161 cgccgaaagg agcatcgggg caatggaggc caggaagtgt cggcaccgcc gcgtggacag
     2221 gaacggcacg cgctcttcgg cgagcgacat gaggttgcgg taggcctgct ctggattgct
     2281 gaagcgatga cggcttaaga cctgcttgat aaagtccggg gatggctcgg gatcgagcac
     2341 caattccacc tctggggatg tctccgtttc atcgcgaaac gcatcatgga gcaggtggtt
     2401 aatgatttgt cgattgcgga aggtgaagcg atagtaatct gcctcaaagg cctcgctggc
     2461 cgacctcgta ggcgtatcgt gatagcccat ccgcacggcg agtttttgga tttcttccgc
     2521 gccctggggg atcgtgtgcg tctgtaagtc gaacatgatc tggaggcgat gttcgagttt
     2581 gcggagaaag tgatagctct cttgaagata agcgctctcc gtgtgagtca agcaaccagc
     2641 ctgttccagc ccagcaatcg catcgagcgt gttgccggtc cgcacgccgg ggagatcggc
     2701 accgtgaaga agctgcaaaa actggatgac aaattcgatg tcgcggatgc c
//
LOCUS       DAOPTR010000512         2746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_29427_length_2746_cov_3.120773, whole genome shotgun sequence.
ACCESSION   DAOPTR010000512 DAOPTR010000000
VERSION     DAOPTR010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29427_length_2746_cov_3.120773"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="PKI05_10055"
     CDS             <1..723
                     /locus_tag="PKI05_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52588.1"
                     /translation="AKRVGFVPFDYSLAGLEGDEAWREIPKRFVYPEVEFAPPPPPAP
                     PLTMEESFELMPVGHPPRDGQVNTERKGDAILVVNERASDGRRALVFRDAEGLAHAFN
                     PHWVINPNHTSGKTSLQFDIWMGSGAILFHEWRTWDTGPYRVGPSLWIQNGQLTVAGK
                     KLMDIPLEQWVRIEVSCAVGTSSDGTWTLRVTLPDGSSKVFDSLPLGNKEFRDLTWIG
                     WCSMAQQESVFYLDQVKLERQP"
     gene            893..1387
                     /gene="greA"
                     /locus_tag="PKI05_10060"
     CDS             893..1387
                     /gene="greA"
                     /locus_tag="PKI05_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007339475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOA52589.1"
                     /translation="MDERIPVTQAGYEKLCRELRELEDQLAEVTKRVAAAREEGDLRE
                     NAEYHGARETQGLVMAKINYLRDKIARCEIVDPSRIPRDRVALGCTVTVRDLDKGFVE
                     RYTLVGVGEEDYDLDRIPVNSPLAKGLLGKRVGEIAEVDVPAGLRRLEIVELAYDDES
                     VMGG"
     gene            1589..>2746
                     /locus_tag="PKI05_10065"
     CDS             1589..>2746
                     /locus_tag="PKI05_10065"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA52590.1"
                     /translation="MFTRSPRRRFLKISAALASAPFAATIVASFAESPNEKLNIAAIG
                     VRGRGAANLAGVASENIVALCDVDANNLNEAAKDFPNAKLYRDFRRMFDEIAKEIDAV
                     VVSTPDHTHAPAAAAAMQLGKDCYCEKPLTHCVYETRYLTELAIRNKLVTQLGTQIHA
                     GDNYRRVVELVKANVIGTISEVHVWSAASYSGGDRPKDTPPVPPYLDWDLWLGPAPYR
                     PYHPAYVPGRWRGWKDFGNGGLGDFFCHYSDLAFWALDLKYPTSVEAEGPEPHPESTP
                     PWLIVRYEFPARNDLPPVRLTWYDGGKRPPIQGEAKLPDWGSGVLFVGEKGMLLADYG
                     RRMLWPEEKFADLEPPPQTIPPSIGHHQEWIQACKTRGTTTCRFEYSGPLTE"
BASE COUNT          609 a          661 c          857 g          619 t
ORIGIN      
        1 gcaaagcggg ttgggtttgt gccttttgat tattctttag cgggacttga gggcgatgag
       61 gcatggcgcg aaataccgaa gcggttcgtt tatccagaag tggagtttgc cccaccccca
      121 ccgccagccc cgcccttgac gatggaagaa tcgttcgagc tgatgccggt ggggcatcca
      181 ccgcgagatg gccaagtgaa caccgagcgt aagggcgatg ccatcctggt ggttaatgag
      241 cgggcctcag atgggcgtag agccttagtt ttccgcgacg ccgagggttt ggcccatgct
      301 ttcaatcccc attgggtgat caatccaaat cacacgagcg gaaaaacatc gttacagttt
      361 gacatttgga tgggaagcgg tgccatcctc tttcatgaat ggcgaacctg ggacacaggg
      421 ccttatcgag ttggaccgtc cctttggatt caaaatggtc aattgaccgt ggcgggcaaa
      481 aagctgatgg atattcccct ggagcagtgg gtgcggattg aggtatcgtg cgcagtgggc
      541 acatcgagcg atggaacctg gacgcttcgc gtcacacttc ctgacggaag cagcaaagtg
      601 tttgatagtt tgcctttggg taacaaggag tttcgcgatc tgacgtggat cggctggtgc
      661 agcatggccc agcaagaatc ggtcttctat ctggatcagg tcaagctgga acgtcaaccg
      721 tgacaaaaca gcagggatta ttcgcggacg agcggtaggc gtgcaaccag gtatgaggag
      781 ggtgaggacg aggtgatgtc ctaccatgca ggattatttt gaacggtcag tgtcatagtg
      841 aagctttggt ttgggagaga gtatcctgga gtctttgagg gagagaaatc ggatggatga
      901 acggattccc gtaacacagg cgggttacga aaagctttgt cgagagctga gagagttgga
      961 ggatcagctt gccgaggtga cgaagcgtgt ggccgcggca agagaggagg gcgatctgcg
     1021 ggagaacgcc gaatatcacg gcgcgcggga gacacaaggg cttgtgatgg cgaagatcaa
     1081 ttatctccgc gacaagattg cccggtgcga aattgttgat ccttctcgga taccgcgaga
     1141 ccgtgttgcc ttgggatgta ctgtaactgt ccgcgatttg gataaaggtt tcgttgagcg
     1201 atacaccctt gtcggtgttg gcgaggaaga ctacgacctg gatcgaattc ccgtcaacag
     1261 cccgctggcc aagggtttgc ttgggaaacg ggtgggggag atcgccgagg ttgacgtccc
     1321 ggccggtctt cgccgcttgg agatcgtgga gctagcctac gatgatgaat cggttatggg
     1381 cggctaaaat ggggcggcga tgtcttgcga caggtgcttc tgccgagatg gcaagggttt
     1441 gtccaagagg gtccttcctc tgctgcttga gttctgccgc ttgttttcct aagataggga
     1501 cacccaattc ggttagtgga acacatccta taaactgcct catagctggg aactttggca
     1561 gactgcccgt tttttatgga gctaagctat gttcacgcgt tccccgagaa gacgctttct
     1621 gaagatttcg gcggccttgg ctagcgcccc gtttgcggcc accattgttg cgagctttgc
     1681 tgaatctcct aatgagaaac tcaacatcgc ggcgatcggt gtgcgaggcc gtggggcagc
     1741 gaatctcgcc ggtgttgcaa gcgaaaacat tgtggcgctg tgtgatgtgg acgccaataa
     1801 tctcaacgaa gcagcgaaag actttccgaa cgccaagtta taccgtgact ttcgtcgcat
     1861 gttcgatgaa atagctaaag agatcgacgc cgtggtggtg agcacgccag atcacacaca
     1921 cgccccggcg gctgctgcgg cgatgcaact tggcaaggac tgttattgtg aaaagccgct
     1981 tacgcattgt gtctatgaga cgcggtattt gacggagttg gccatccgaa ataaattggt
     2041 gacgcaattg ggaacgcaga tccacgctgg ggacaactat cgccgcgtgg tcgaattggt
     2101 caaggctaac gtaatcggga cgatttcgga agttcacgta tggagtgcgg cgagctacag
     2161 cggaggcgat cggcccaaag atacgccacc agttcctccc tatttggatt gggacctttg
     2221 gcttgggcct gccccctatc gaccatatca tcctgcttat gtgccgggcc ggtggcgtgg
     2281 ctggaaggac tttggcaacg gagggttggg cgatttcttc tgccactata gtgatttggc
     2341 cttctgggca ttggatttga agtatcccac gtccgtggag gcagaaggtc ccgaaccgca
     2401 ccctgagagc acgccgccgt ggttgattgt ccgatacgaa ttccccgcac gaaatgatct
     2461 cccacccgtc cgtttgacgt ggtacgacgg cggaaagagg cctcccatcc aaggcgaggc
     2521 caagctgccc gattggggct caggagtcct gttcgttgga gaaaaaggga tgctgttggc
     2581 ggactacgga cggcggatgc tctggcccga agaaaaattc gccgatctcg aaccgccacc
     2641 gcaaacgatt ccaccctcta tcggtcatca tcaggagtgg atacaggctt gtaagacgcg
     2701 cgggacaaca acatgccggt tcgagtactc ggggccctta acggaa
//
LOCUS       DAOPTR010000513         2744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_29463_length_2744_cov_3.036817, whole genome shotgun sequence.
ACCESSION   DAOPTR010000513 DAOPTR010000000
VERSION     DAOPTR010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29463_length_2744_cov_3.036817"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PKI05_10070"
     CDS             complement(<1..322)
                     /locus_tag="PKI05_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52591.1"
                     /translation="MLHLLFLPTPAEKTNDHAKTAFRGAMNPAVVRGNTLARRQMYRA
                     AVKNAKVLLPLLELLESRLDRQQKNISIRPDVQANKTGTLEPIPKFPLPLGEGWGEGK
                     PSSES"
     gene            321..638
                     /locus_tag="PKI05_10075"
     CDS             321..638
                     /locus_tag="PKI05_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52592.1"
                     /translation="MTKTAVLQAQQRWEYVFIQRRSELALQDELNVMGQQGWELVHVE
                     YYKDAKGIMGWIAFLKRPSAASKPPGTGAGEHEVMHEKEPTPGGVQRPMPPPDDVFEV
                     KTE"
     gene            complement(777..2213)
                     /locus_tag="PKI05_10080"
     CDS             complement(777..2213)
                     /locus_tag="PKI05_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52593.1"
                     /translation="MECQDLRQEATRQRRCDGWWKSARTSQGGERLGRVIPWRLAIFG
                     LCLSLVVAGCRQASQPVAPSPSPLEELGRLPGLRRSTHPQLSEELARIEEEGGTPEQL
                     TVPLPPREDNAASALVALFPKERVEFLVAQTSDWLRVAPDRLQKEELQAAKSLLQSFQ
                     TQRATCRAALLRPKCVFPIRFTAGFGADLSFVDQCVLAVQIEHLAAALAISEGNLDAA
                     VDALNVALRLCGVLSRTKHPLLRWQAASLRRQTLDCLPTLVSHPEITRDALGQIADTL
                     QKELAIWPSDAETWIGARALGMHAYELVRIGRLYDVLTEREIKQLRQSYDLEELVRTT
                     QEHADQDEFYYLTQMREIIKHSQKPYCERAGELEKLQAAWEHKSHDDETVVACRVLLP
                     EVPTGQKTQAEDLANVCAWLVALRAAREDIQSWDTVNPLTGRPFIVEVQDDTVYVSGI
                     GTDIGLHGAIGVPRRPPPKGSQPKREQT"
     gene            complement(2393..>2744)
                     /locus_tag="PKI05_10085"
     CDS             complement(2393..>2744)
                     /locus_tag="PKI05_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52594.1"
                     /translation="TVREEEDPTRDLPLSALYRWQSALQVPLIELLSDIEEPLSAPVL
                     KRARLLRMMKTVRAIQLRAKQPVVRQLAELLAEQLLEIMPELADVSPWPMGRRRRSSD
                     CGPTADRIFKVRFR"
BASE COUNT          585 a          793 c          794 g          572 t
ORIGIN      
        1 tcgactccga cgatggcttg ccctcacccc aaccctctcc cagagggaga gggaactttg
       61 ggataggttc taaagtgcca gttttgtttg cctggacgtc gggacgtatg gagatattct
      121 tctgctggcg gtctaaacgg ctctcaagaa gttccaaaag cggcaacaaa acctttgcat
      181 ttttgacagc ggcgcgatac atttgacggc gcgccagggt attgcctcgt acgacggccg
      241 ggttcattgc accgcggaac gccgtcttag cgtggtcatt ggttttttcg gcgggtgtgg
      301 ggaggaaaag gaggtggagc atgaccaaga ctgccgtact tcaggcgcag cagcgctggg
      361 agtatgtgtt catccagcga cgatcagagc tggcccttca ggacgaatta aacgtcatgg
      421 ggcagcaggg atgggagtta gtgcatgttg agtattacaa ggatgcgaag ggaatcatgg
      481 gctggattgc ctttttgaag cgccccagcg cggccagcaa gccaccgggc acgggtgccg
      541 gcgaacacga agtcatgcac gaaaaggagc caaccccagg tggtgtgcaa cgccccatgc
      601 caccccctga cgacgtgttt gaggtcaaaa cggagtaagc gccccacatg tgatgccttt
      661 aggccgaatc gatttggtcg ttccgtgctc gacaggagac gatacggcca gccgtcacaa
      721 cattcccacg atccgcactt gcggtggcaa ttcgggcgga gacctgggtg actggatcat
      781 gtttgctcgc gtttaggctg gcttcctttg ggcggtgggc gtcgggggac tccgatagca
      841 ccatgcaggc caatgtccgt gccgatccca ctcacgtaaa cggtgtcgtc ttgcacttct
      901 acgataaacg ggcgacctgt cagcgggtta acggtgtccc aactctggat gtcttcccgg
      961 gccgctcgca acgccaccaa ccacgcgcat acattggcga ggtcctcggc ctgggtcttc
     1021 tggcccgtgg gaacctccgg aagcaagacg cgacacgcca caaccgtttc gtcatcgtgg
     1081 gacttatgct cccaggctgc ctgaagtttt tcaagctcgc cggccctttc gcagtaaggc
     1141 ttctgggaat gcttgataat ctcgcgcatt tgggtcaagt agtaaaactc gtcttggtcg
     1201 gcatgttcct gtgtggttct cactaactct tccaaatcgt agctttggcg caattgtttg
     1261 atctcgcgct ccgtgagaac atcatacagc cgaccgatgc gaaccaactc gtaggcatgc
     1321 attcccaaag ctcgggcacc aatccaggtc tctgcatcgc tgggccaaat cgcgagctcc
     1381 ttttgcagtg tgtcggcaat ttgccctagc gcatcccgtg tgatctcggg gtgcgacacg
     1441 agcgtcggaa ggcaatcgag cgtttgtctc cgtaaggacg cggcttgcca ccgcagcagc
     1501 gggtgttttg ttcgagagag gacaccacac aggcgaaggg ccacgttgag cgcatccacg
     1561 gccgcatcca gattgccctc ggagatggcc aaagcggcgg ctaggtgctc aatctgcacg
     1621 gccaagacgc attgatcgac aaagctcaga tcggcaccaa atccggccgt aaagcggatg
     1681 ggaaagacac acttcggacg caacaaggct gcgcggcatg ttgcccgctg cgtctgaaat
     1741 gattgtaaca gcgatttagc ggcttgaagc tcttctttct gaagacgatc cggtgccacc
     1801 cgaagccaat cgcttgtctg agccaccagg aactccactc gctctttcgg aaataatgcg
     1861 acaagtgccg aggcagcatt gtcttcgcgc gggggcaaag gaacggtcaa ctgctcaggc
     1921 gtaccgcctt cttcctcaat gcgggccaat tcctcgctca attgaggatg ggtcgaacgg
     1981 cgtaggccgg gcaggcgtcc cagctcctca agtggagacg gcgaaggtgc caccggctgg
     2041 ctggcctgac ggcatcccgc gacaacgaga gaaagacaca aaccgaaaat cgccaatcgc
     2101 cacggaataa cacgcccgag acgttcaccg ccttgggacg ttctcgcgga cttccaccaa
     2161 ccatcgcacc gacgttggcg cgtcgcttcc tgccgtaagt cctgacactc catcaactcg
     2221 gctccttcaa ggtggtcccg cggatccgat gatcaccact ctcgttgtat gatatcacct
     2281 tttaggagcc gagaaagttc cgatactgcg cgcacagttt gactaaactg acacggcgcc
     2341 tttccccaaa gcgtgggcac accgcgggcc gtatcaagct gctaattgcc gattaccgaa
     2401 accggacctt gaaaatgcga tccgccgtgg ggccacagtc gctgcttcga cgacggcggc
     2461 ccattggcca cgggctgaca tcggccagtt cgggcatgat ttcaagtaat tgttctgcca
     2521 gaagttcggc caattgccgc acgacaggct gttttgcccg aagctgaatc gcccgcaccg
     2581 tcttcatcat tcgcagcaaa cgggcacgtt tgaggaccgg cgccgacagc ggttcctcga
     2641 tgtcggaaag cagttcaatc aggggaacct gcaaggcact ttgccagcga tatagtgccg
     2701 aaaggggaag gtctcgcgtc ggatcctctt cttctcggac ggtg
//
LOCUS       DAOPTR010000514         2743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_29475_length_2743_cov_3.080729, whole genome shotgun sequence.
ACCESSION   DAOPTR010000514 DAOPTR010000000
VERSION     DAOPTR010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29475_length_2743_cov_3.080729"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PKI05_10090"
     CDS             complement(<1..653)
                     /locus_tag="PKI05_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008694931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOA52595.1"
                     /translation="MRRRVVVTGMGMVTPLGADLETVWRRLLNGESGIDYITLFDASH
                     FPTKIAAEVKNWDLSDVGENPEDWKFQGRHTHFAIGAAKKAWQDSGLEESKIDPTRFG
                     IYTGSGEGQQDFDRFTQMMVAALVDGELDLVKFTKKGLEILHPIVELEQEPNMPAGHL
                     AALFNAQGPNINCLTACAASSQAIGEAVEIIRRGEADIMLSGGTHTMIHPFGVTGFCL
                     "
     gene            complement(796..1311)
                     /locus_tag="PKI05_10095"
     CDS             complement(796..1311)
                     /locus_tag="PKI05_10095"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HOA52596.1"
                     /translation="MRWYWIDRFIEFESGRYAKAVKNVSLAEEHLHDHFPGYPIMPPS
                     LIIEGVAQTAGLLVGEYNQFKMKVVLAKVPRAVFYHEAKPGDTLIYTATIDAIHPHGA
                     AVHATSHLGDKLQAELELVFAHLGDEFAGRQLFRPGTLTHMMRILRAYEVGRAADGSP
                     LVEPPDDRDAP"
     gene            complement(1406..1801)
                     /locus_tag="PKI05_10100"
     CDS             complement(1406..1801)
                     /locus_tag="PKI05_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOA52597.1"
                     /translation="MLTKEEIYQKVREVLMDALAVDEEEVRPDATLVGDLGAESIDFL
                     DIVFKLEKAFNIKIPRGELFPEDILTNPQFVKDGKFTEAGLEELRKRMPFAELDQFAR
                     NPLVQDFANLLTVADMCRFVELKLQQQGG"
     gene            complement(1925..2683)
                     /locus_tag="PKI05_10105"
     CDS             complement(1925..2683)
                     /locus_tag="PKI05_10105"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HOA52598.1"
                     /translation="MTQRFAGKVALVTGGSRGIGRACVAELAKGGAEVWFIYQSNQLA
                     AAGLAGELADQGATVHPEQADVRDFERIQQLAERIVNQCGKIDILVNSAGIVRDGLLG
                     TMTAEQWNEVLAVNLTGTFNCCRAVAQFMLSQRSGSIVNLSSTAAEFSSRGQVNYAAS
                     KGGINGFTRALAKELAPRNVRVNAVAPGMIDTDMSEAVRGLAGNKIKEIIPMRRIGTP
                     EEVAKLVAFLASDDASYITGQVIRVDGGLSLGGY"
BASE COUNT          559 a          816 c          737 g          631 t
ORIGIN      
        1 aggcaaaatc ccgtgacccc gaaggggtga atcatggtat gcgttccacc cgaaagcatg
       61 atgtcggcct ctccccgacg aatgatttcc accgcctcgc cgatggcttg gctagatgcc
      121 gcacaggccg tgaggcaatt aatgttgggg ccttgcgcat tgaacagggc ggccaagtgt
      181 cccgccggca tattgggttc ctgctctagt tccacgatgg ggtggaggat ctccaggccc
      241 ttttttgtga acttgacgag gtccaattcg ccgtcaacca aagcggccac catcatttgg
      301 gtaaagcggt cgaaatcctg ctggccttcg ccgctacctg tgtaaatgcc aaaccgcgtg
      361 ggatcgatct tcgattcctc aagcccggaa tcttgccagg cctttttggc ggcgccgatg
      421 gcgaaatggg tatggcgtcc ctggaacttc cagtcctcag ggttttcccc gacatccgaa
      481 aggtcccagt ttttcacttc ggcggcgatc ttcgtgggaa aatggctggc atcgaagagg
      541 gtgatgtagt cgatccccga ctcaccgttg agcagacgac gccaaaccgt ctccaggtcg
      601 gcgcccaacg gtgtgaccat acccattccc gtaacaacaa cccgacgtct catctgatat
      661 catactccgt aaggatagga cacttgagtg tcttttgcct cgatcgcaaa tgtgtggtgg
      721 catccctacg atggataggc aggccgtctt ggccgagccg catcgtctgg cccatgcgat
      781 tcaccccaca gaatgctatg gggcatctcg gtcatcgggc ggttccacga ggggggaacc
      841 gtcggctgcc cgccccactt cgtacgcccg caaaatccgc atcatatggg tgagtgtccc
      901 tggtcggaac aactgccgac ctgcgaactc gtcacccaaa tgggcgaaca ccaattccag
      961 ctctgcctga agtttatctc cgaggtggct ggtggcgtgg acggccgccc catgggggtg
     1021 gatcgcgtca atggtggcgg tgtagatcaa cgtgtctccc ggtttggcct cgtgataaaa
     1081 gacggcccgg gggactttgg ccaagaccac cttcatcttg aattgattgt attcccccac
     1141 caaaagaccc gccgtttggg cgacgccttc gatgatcaaa ctggggggca tgatggggta
     1201 gcctggaaaa tggtcgtgga ggtgttcctc ggccaaggag acgtttttca cagccttggc
     1261 gtaacgacca ctttcaaact cgataaatcg atcgatccaa taccagcgca tacgttttgg
     1321 cttttcctat cagcggcaaa gcaagcgacc cggcagcatc ccgcccagtc gcccgcagcc
     1381 tggatgcccc ccggcgggtc gccaatcagc cgccttgctg ttgcagctta agctcgacaa
     1441 agcggcacat atcggccacc gtgagcaggt tcgcgaagtc ctgaaccaac ggatttcggg
     1501 cgaactgatc aagctccgcg aagggcatac gcttgcgaag ttcctccaga ccggcctcgg
     1561 tgaatttgcc gtccttcaca aattgcggat tggtcaaaat gtcctcaggg aataactcac
     1621 cccgcggaat tttgatgttg aaggcctttt ccaatttgaa gacgatgtca aggaaatcga
     1681 tggattctgc gccgagatcc cccacaagag tggcatcagg gcgtacttct tcctcgtcca
     1741 ccgccaaggc gtccatgagg acttcccgta ccttttggta aatctcctcc tttgtcagca
     1801 tttggagtct ccctttgacc agtccgaagt cgcaaattgg aaaacgtttc ccgataatcg
     1861 tttcatttag ccgaaatttg tcgttttgcc tagtctaggt agtccccttt ctggggaaga
     1921 attgttaata cccccccaag ctcagtccac cgtccacacg gatcacctgg ccggtgatat
     1981 acgacgcatc atcgctggcg aggaaggcca cgagtttggc gacttcctcg ggcgtgccga
     2041 tgcgccgcat agggataatc tccttgattt tgtttcccgc caaaccgcgg acggcttcgc
     2101 tcatatcggt gtcgatcatg cccggggcca cggcattgac gcggacgttc cgcggggcca
     2161 actccttagc caatgcccgc gtgaaaccat tgatgccacc cttactggcg gcgtaattga
     2221 cctgtcctcg tgaagaaaac tcggccgcgg tgctggagag gttgacaata ctcccgctcc
     2281 gctgggacaa cataaactgg gccaccgctc gacagcagtt gaacgtcccc gtcaggttga
     2341 ccgccaaaac ctcgttccac tgttcggccg tcatagttcc cagcaggccg tctcgaacga
     2401 ttcccgccga gttgacgaga atgtcgatct ttccgcattg gttgacgata cgttctgcca
     2461 attgttgaat gcgttcaaag tctcgcacgt cggcttgctc tggatggacg gtcgcccctt
     2521 ggtcggctaa ctcgccggcc aaaccggctg ccgctagttg gttgctctgg tagatgaacc
     2581 agacctcagc cccgcccttg gccaattctg caacacatgc ccgaccgatc ccccggcttc
     2641 caccggtgac aagggcgact tttcctgcga atcgttgtgt catgttggca tatcctctcg
     2701 gcttgatccc attttcaact ggtgatttgt tcagtcagtg cga
//
LOCUS       DAOPTR010000515         2743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_29476_length_2743_cov_3.026042, whole genome shotgun sequence.
ACCESSION   DAOPTR010000515 DAOPTR010000000
VERSION     DAOPTR010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29476_length_2743_cov_3.026042"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1022)
                     /locus_tag="PKI05_10110"
     CDS             complement(105..1022)
                     /locus_tag="PKI05_10110"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOA52599.1"
                     /translation="MTQTQATDREFGFIGIGQMGRAMVENWLALGYRVRVYNRTREKI
                     LPLLEKGAVEAPTPADAVVPSGIVMSCVSDDPSLDQIFSDLEIFRRLGSDGVHVSMST
                     ISPKMARLLAERHKQHGGVYVAAPVMGRPDTVALRRQTFYLSGPTKARERVKPLLVPI
                     CRQVLELGESAEAAHVAKLASNFLIATVVESLGEAFAFVEKNGLSPQTFYETITEFLF
                     DCFIYKGYGRHLLSGQFREPLFRLQLGLKDIQLSLQSATESRTPMPILSLLRDRYLTA
                     VAQGYGDWDWTAINLEIRRQAGLAHDQGQ"
     gene            complement(1390..2208)
                     /locus_tag="PKI05_10115"
     CDS             complement(1390..2208)
                     /locus_tag="PKI05_10115"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOA52600.1"
                     /translation="MVRFSNHKDHHFLLRLPGNESLMCEQVDVGVRLARLPKHLEGMT
                     ILLLSDLHFTGKIGLDYYHQVVEHTNKLQPDLIFVTGDIVDRSDCIGDAATVLSELRA
                     QHGVFFVLGNHDLRAGPLAVRTALSQAGLTDVGGRVVRLPVDGIAFLIAGNELPWLGP
                     APDYSAAVNGDRHPPLRLLLAHTPDQFSWAVRQGVDLMLAGHTHGGQICLPWLGPIIV
                     PSAGGIRYAHGLHYRRPTAMYVTRGLAGEFPVRYFCPPELTRIILFSGNRAQKD"
     gene            complement(2456..>2743)
                     /locus_tag="PKI05_10120"
     CDS             complement(2456..>2743)
                     /locus_tag="PKI05_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52601.1"
                     /translation="GCAGRNNPNGPCGVGPPQGSAIVSGLFYRQKPADHYGFVTTSYF
                     CPHLRSGVLVGPSVRCDLSNLAAEQAKTASSSFHGSRIGLRCTPARHELAA"
BASE COUNT          652 a          746 c          803 g          542 t
ORIGIN      
        1 cccccggaaa gtgactgtcg cggcgagacc ggcttttacc tcgtgtagcg tgaaagttcc
       61 gaggtggaaa gacattggcg gcacggcggc tgttgatcaa tcggtcactg accttgatca
      121 tgggccaagc cggcctgccg gcgaatctcg agattgatgg ccgtccagtc ccaatcccca
      181 taaccttgtg caacggccgt caaataacga tcccgcaaaa gactcaagat cggcattggg
      241 gttcgtgact ctgtggccga ttgcaacgag agttggatat ccttgagtcc caattggagg
      301 cgaaagaggg gttcgcggaa ctgaccgctc aagagatgtc ggccatagcc tttgtaaata
      361 aagcaatcga agaggaattc cgtgatggtc tcgtagaatg tttgcggtga taggccattt
      421 ttctctacaa acgcaaaagc ctcacccagc gattccacga cggtggcaat gaggaaattc
      481 gaggccaact tcgccacgtg ggcagcttca gcggactccc ctaactcgag gacctggcgg
      541 caaatcggta ccagcagggg tttcacgcgt tcccgcgcct tggtggggcc ggaaaggtag
      601 aatgtctgcc gacgtagggc aacagtgtcg ggacgaccca tcacgggtgc cgccacatag
      661 acgccaccgt gctgcttgtg ccgctcggca agaagccgcg ccatcttcgg agaaatcgtg
      721 ctcatggaaa cgtgaacgcc gtctgacccc aatcgccgga agatctccag atcgctgaag
      781 atctgatcga gcgatgggtc gtcagagacg caggacatga cgatgcccga cggaacaacg
      841 gcatcggcgg gcgtgggggc ctcgacggcg cctttttcga gcagtgggag aatcttctca
      901 cgggttcggt tgtaaacgcg tacccgatac ccaagggcta gccaattttc gaccatggca
      961 cgccccattt gcccgatgcc aataaaaccg aattcccggt cggtggcctg tgtctgcgtc
     1021 atttgccgac cctccaagat tacctattca caaaaccgcc ttacatgcgc tgcgcacata
     1081 tctgcaatga taacagtaaa aggggagaag tcggagggac acgggttatg gcggagaaga
     1141 cactgaatac agcatagaac ctatcccact gcctcccctc gcccgttcgg tgggagaggg
     1201 gtcgggggag agggtggctc gaaccgctgc tggttttctc gatcgactcc gacgatggct
     1261 tgccctcacc ccaaccctct cccagaggga gagggagttt tgggatagac tcacactagg
     1321 aattaagaca accgtacaac ttcaagccgg cgcggtgaga atgcccaata gccggctata
     1381 agtgtccgat caatcctttt gtgcgcggtt gcccgagaag agaataatgc gtgtcaattc
     1441 gggtgggcaa aaatatcgga cggggaattc ccccgccagg ccccgtgtga cgtacatcgc
     1501 cgttggccgt cggtaatgaa gtccgtgtgc atagcggatg cctcccgcac ttgggacgat
     1561 gattggaccc agccagggca ggcaaatctg cccgccgtgg gtatggccag ccaacattaa
     1621 atcaacccct tgacgcactg cccaggagaa ttggtccggc gtatgagcta agagtaatcg
     1681 taaagggggg tgacggtctc cgttgaccgc cgcggaataa tcaggtgccg gtcccaacca
     1741 gggaagctca tttcccgcga taagaaatgc aataccatcc accggaaggc gaaccacgcg
     1801 tccgcctacg tcggttaacc cggcctgcga gagggcggtg cgaaccgcca agggaccggc
     1861 ccgcaggtcg tgattcccta gcacgaagaa aacaccatgt tgtgcccgca gctccgacaa
     1921 gacagtggcc gcatcgccga tgcagtcaga acgatcaaca atgtcgccag tcacgaagat
     1981 caggtccggt tgtagcttat tcgtgtgttc aacgacttga tggtaataat ccaagccaat
     2041 cttgcctgtg aaatgcaagt cggaaagaag aagaatcgtc attccttcga ggtgcttggg
     2101 aagacgggct agacggacac cgacatcgac ctgttcgcac atgagcgatt cgtttcccgg
     2161 cagacgcaaa aggaagtggt gatccttgtg gttcgaaaag cgaaccactg gggaccggtc
     2221 tgcttgggaa tctcgaggtt ctcctgtcct accccgcgac tggtgatttc tttctggaaa
     2281 aaaggatggt cgtacttggt gcagggaacc cactgtgggg agccaaacag gagattttct
     2341 ctcctcaccg aggctttgtt ggcctgcgtg actaaaaacc ttgcgagcag cgacgaagtt
     2401 tttcaaagaa gggagccacc ttcccgaggt gggtcgtcgc gagatcttgc tacggtcagg
     2461 ccgccagttc atgacgggcc ggcgtgcacc gaaggccaat tcttgagccg tgaaatgatg
     2521 aactggcggt tttcgcttgt tccgccgcca aattcgagag atcacagcgt acggaagggc
     2581 caaccagcac gccgctacgt aggtgtgggc agaaataact agtggtgaca aacccatagt
     2641 ggtcggcagg tttttgccta tagaaaagac cggaaacaat ggcagatccc tgcggaggac
     2701 caacaccaca agggccgttg ggattgttgc gcccagcgca acc
//
LOCUS       DAOPTR010000516         2732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_29637_length_2732_cov_3.031378, whole genome shotgun sequence.
ACCESSION   DAOPTR010000516 DAOPTR010000000
VERSION     DAOPTR010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29637_length_2732_cov_3.031378"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="PKI05_10125"
     CDS             complement(<1..666)
                     /locus_tag="PKI05_10125"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOA52602.1"
                     /translation="MHATLKGQDLAKLVWNELIRVVGLSLTWTTLFAIAMGADDRTFK
                     AFSLQHASPGQVESVLRSLVSPGTEIIQDTAGRRILISGPETDLQIAASVVAALDRPQ
                     ETSPQPPVAKPAVSQIKSYFVSRDRLQSMLAILQAQFANRSDVRITADERTSQILVLG
                     PQDVHASVGGLLALGPTPSQTGTTSQGRTPLSSSNPSANLPAGKAQQSGTGTSSSGRT
                     FPAQ"
     gene            complement(940..>2732)
                     /gene="mutS"
                     /locus_tag="PKI05_10130"
     CDS             complement(940..>2732)
                     /gene="mutS"
                     /locus_tag="PKI05_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOA52603.1"
                     /translation="EARREGSLLWVIDRTITGMGGRLLIDWLTNPLLDQKAIVKRQDA
                     VAEFLNRPEICSKVRQALARVFDLERLLGRATTGRCTPRDLASIARTLRQIPPLKEIL
                     AKCYGQRLGEIAQLLDPCPELEAKLSAALVEQCPLSATEGGFIREGYHAELDELRRLA
                     QGGKQWIAAYQAKEIERTGIPSLKVGYNKVFGYYIEVTNTHRDKVPADYFRKQTLKNA
                     ERYITPELKEYEEKVLSADERAKQLEYEIFVELREFLLQFRSTLQRTAALLAELDVLA
                     GWAELARERNYCRPELCEEPILEITEGRHPVLDITQPEGTFVPNDLRCGPECFIILIT
                     GPNMAGKSTYIRQTALITILAQIGCFVPAKSARIGIVDRLFARVGASDELARGRSTFM
                     VEMIETARILNSATKRSLVILDEIGRGTSTYDGMSLAWAIVEYLHNRIGCRTLFATHY
                     HELTELAEVLPGVKNFNVAVREWQDQVIFLHTIVPGAADKSYGIHVAQLAGVPRVVIE
                     RARQILEDLETKHLEGPNPFRQVTSSSSSGQGRPIQLTLFEPIDHPLMEEIRELDLTK
                     ITPLEAFEKLRKWQEQLASRPTKRRGWRST"
BASE COUNT          603 a          759 c          735 g          635 t
ORIGIN      
        1 ttgcgccggg aaagtgcgtc cgctagaact tgttccggta cctgactgct gggcctttcc
       61 cgcgggtaga ttcgccgatg gattggaact tgacaagggt gttcgtcctt gcgaagtggt
      121 acccgtttgg cttggggtgg gccccagtgc caatagacca cccacggatg cgtgaacatc
      181 ttgaggtccc aaaacaagga tctggctagt tcgctcgtcg gctgtgatcc gaacgtccga
      241 ccggttggcg aactgtgcct gaaggatcgc cagcatcgac tgcaaacggt cacgagagac
      301 aaaataactc tttatctggg aaacagccgg ttttgccacc ggcggctgcg gcgaagtctc
      361 ctggggccta tccagtgccg cgacaacact ggctgcgatt tggagatcgg tctcgggccc
      421 agaaatgagt atgcgacgcc ctgcggtgtc ttgaattatc tctgtgccgg gtgacactaa
      481 agaacgcaag actgactcga cctgcccagg ggatgcgtgc tggagactga acgccttaaa
      541 tgtgcgatcg tcagccccca tggcgatcgc aaagagggtc gtccaggtca agctgagtcc
      601 cacaacacga attaattcgt tccataccag tttggcgaga tcctggcctt tcaaagtggc
      661 gtgcatgctt gatccccaaa agtcttatcc atcctcaaca gttcaccaaa acaatagacg
      721 caacaccacc gttattgccc gtgttactga tacaatcgac aaatcttctc aggcaattga
      781 ggatgacttt tccggttagt aggggttttc ggcattcaac ccacacttac cgaagctcca
      841 aggctcaatg cgataaataa gatgtgagtg tgaggcgaat gcgacaaatg ggcaagataa
      901 gttcaaagaa aaaagcggcg agatatgaga agcccatggt taagtcgatc gccacccgcg
      961 gcgtttggta ggccgcgatg ccaattgctc ctgccacttt cgcagtttct cgaaggcttc
     1021 caggggcgta atcttcgtca aatcgagttc gcgaatctcc tccatcaaag gatggtcgat
     1081 gggctcaaaa agcgtcagtt ggatgggccg accttgtccg ctggatgatg aagaggtaac
     1141 ttgccggaac gggttggggc cttccagatg ctttgtctct aggtcttcca aaatctgcct
     1201 cgccctctcg atgaccacac ggggcacacc tgccagttgg gccacatgga tgccgtaact
     1261 tttgtctgct gctcctggca caatggtgtg caagaagatc acctgatctt gccactcccg
     1321 gactgccacg ttgaagttct tgaccccggg caacacttcg gccagttctg tcagttcgtg
     1381 ataatgtgtg gcgaaaagtg tccggcatcc aatgcgatta tggagatact caacgatggc
     1441 ccaggccagc gacatcccgt cgtaggtact ggtcccgcga ccgatctcat ccaagatcac
     1501 gagactccgc ttggtggccg aattgagaat ccgtgccgtc tcaatcattt cgaccataaa
     1561 ggtacttcga ccccgggcga gttcgtcgct tgcgccaacc cgggcaaaaa ggcggtcaac
     1621 aattccgatc cgtgcactct tggccggcac aaagcaacca atttgcgcaa gaatggttat
     1681 tagggctgtt tgacgaatat aagtgctttt tccggccatg tttggccccg tgatcaaaat
     1741 gataaagcac tcgggaccac aacggagatc attggggaca aacgtcccct ccggttgggt
     1801 aatgtctagc acaggatgcc gtccctcggt gatctccaga atgggctcct cgcacaactc
     1861 gggtcggcaa taattccgct ctcgcgcaag ctcggcccag cccgccagga cgtcgagttc
     1921 ggccaacagc gcggcagttc gttgaagagt actccgaaat tggagcaaga actctcgcaa
     1981 ttcaacaaag atttcatatt caagttgctt ggcccgctca tcggcggaaa ggaccttttc
     2041 ctcgtattcc ttcagttccg gcgtgatgta acgttcggcg ttcttgaggg tttgcttgcg
     2101 aaaatagtcg gccgggacct tatcccggtg ggtattcgtg acttcaatat agtagccgaa
     2161 gaccttattg tatcccacct tgagcgaggg aatccccgtc cgctcaattt ctttggcctg
     2221 gtaagcggcg atccattgtt tgccgccctg ggcgagacgt cttagttcgt ccagttctgc
     2281 gtggtatccc tcccggatga atcccccttc cgtcgcggag aggggacatt gttccaccaa
     2341 tgccgccgac agctttgctt ccagctcggg acatggatca agcaactgag caatttcgcc
     2401 caggcgttga ccgtagcact tggccagtat ctctttgagg ggtgggatct gccgcaaggt
     2461 tcttgcgatg ctcgctaggt ctctgggggt acaacgacct gtcgtcgcgc ggcccagtaa
     2521 ccgttccaaa tcgaataccc tcgccagtgc ctggcggacc ttgctacaaa tctccggccg
     2581 attgagaaac tcagcgacag cgtcttgccg tttgacgatg gctttctggt ccagtagtgg
     2641 attggttaac cagtcgatca aaagcctgcc gcccatcccc gtgatcgttc gatcaatgac
     2701 ccagaggagc gatccttccc gccgagcctc ca
//
LOCUS       DAOPTR010000517         2726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_29723_length_2726_cov_3.453763, whole genome shotgun sequence.
ACCESSION   DAOPTR010000517 DAOPTR010000000
VERSION     DAOPTR010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29723_length_2726_cov_3.453763"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..1226
                     /locus_tag="PKI05_10135"
     CDS             189..1226
                     /locus_tag="PKI05_10135"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOA52604.1"
                     /translation="MKAAFFTGLRQVGILETPPAQIERPQDVLVRVERVGVCGSDVHY
                     FLHGRIGDQILQYPASVGHECAGTVVDVGPKVTRVKVGDLVAIEPALVCNECDQCRAG
                     RSNTCRRLQFLGCPGEAPGAAREYYALPESNCFPLPAGLTLDHGALAEPLSIGLYAVR
                     LAQLQSGDRVAILGCGPIGLSVLTWVKLTAGCRAYVTDLLDERLEAAAQCGADWTGNA
                     AREDVATSIERREPLGVDCVFECSGDAGCVTQAIRLAKPGGRVLWVGIPPEEHVSLPA
                     HLARRKEITLQLVRRQRDCLKPVLHAMAQGQINPTWWITHRYPLNRIQEAFELVAEYR
                     DGVIKAMIELP"
     gene            1582..>2726
                     /locus_tag="PKI05_10140"
     CDS             1582..>2726
                     /locus_tag="PKI05_10140"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOA52605.1"
                     /translation="MAIGEWTTYWTISEWIIRLAMLPVIVMRKERPATCLAWLTVVFF
                     APLPGLLLYLLVGENRLGRRRLEIRKSRWAKLESVNAPPVGAHYQSDSSELWGEPARE
                     DADEADVADDSLQTGTMGKGGLDPELQPLSDLAFRLTGWPVTTENQLSFLCDPLLTID
                     RLVAEIDSANDHVHLLYFIFRDDEIGRRVADALVRAAQRGVQCRVLVDAVGSRPMLKR
                     LAPRLRGAGVEVHPALPVRLYRIPFARLDLRNHRKLAIIDGRVAYTGSQNIVEPTYGR
                     RKAGPWHDITARIVGPSVPHLQLLFLEDWFHETGQWLDEPRYFPVISEGGQTILQVLP
                     SGPDMPTELFQDLLVKAIFLARRRVILTSPYFVPDEPLTMALRLAAYR"
BASE COUNT          554 a          745 c          830 g          597 t
ORIGIN      
        1 acggtcgggg ccggcgatgc atttaccgcg gcctgggtcg cgtcgcgtct tttaggatgg
       61 agtctggcaa aacaggcgga ctttgccaat cgcgtgggcg ccctggtggc atctttgccg
      121 ggtgccatgc cgccagtgaa gcacgagtac gaaaaaatcc tccagacttt agggtgaatg
      181 gagattgtat gaaggcggcc tttttcacgg gattgcgcca ggtgggcata ctggagacgc
      241 cgccggccca aatcgagcgt ccgcaggacg tgcttgtgcg ggtggaacgc gtgggggtat
      301 gcggttctga cgtgcattat tttcttcatg gccgcattgg cgaccaaatc cttcagtatc
      361 cggcttccgt tggccatgaa tgtgcgggca cggtggtaga cgtgggaccg aaagtcactc
      421 gggtcaaggt gggtgacctg gtggcgattg agccggcgct ggtctgcaat gagtgtgacc
      481 agtgtcgggc aggccggtcg aacacctgcc gacggctgca attcctcggt tgtcctggtg
      541 aggcgcccgg agcagcgcgc gagtattacg cgctgcccga gagcaattgt tttcctctcc
      601 cggcaggttt gacactagat catggagcac tggcagagcc gctctcgatc gggctgtacg
      661 cggtgcggct cgcccagctt caaagtgggg atcgggtggc gattttgggc tgtgggccga
      721 tcggattgag tgtcttgacg tgggtaaaat tgacggctgg ctgtagggcg tacgtcaccg
      781 atctgttgga tgaacggctg gaggcagccg cacaatgcgg tgcggattgg accggcaatg
      841 cagcccgcga ggatgtggca acatctattg agcgtcggga accgttggga gtcgattgcg
      901 tgtttgagtg ttccggcgac gcgggatgtg tcacccaagc catccggctt gcaaaacccg
      961 gaggccgcgt actatgggtg ggcatcccac cggaagagca cgtttcgctc cctgcccatc
     1021 tggcacgaag aaaagaaatc accctacaat tggtccggcg acagcgggac tgtctcaaac
     1081 cggtgttgca cgcgatggcc caaggccaaa tcaatcccac gtggtggatc acccatcggt
     1141 acccgttgaa ccggattcaa gaggccttcg agcttgtggc cgagtatcgc gacggggtga
     1201 tcaaggcaat gatcgaactc ccataattgt cccgaaattt ccaaccgttg tgttgcaacc
     1261 ttgttttcgg gtagagtccc cgttaatggg aaattgcccc gattggaaag cacaaaacct
     1321 tgagctgcaa ctgaccataa ctggggcaca accgccaatt cgttggtacg gacttgtaaa
     1381 cgactgccag ccgccgccca ctgctggttg gacggatcgg cacacaagag acagttgaca
     1441 cacaactcgg aacatagcat cctcgcgacg ccgagtatat cacaatggat tggtgtaacg
     1501 ataatgagct gcctggaacc aaatcgaagc agtccttgtg aaaactcatg atttgactgc
     1561 gtatcacttt caagagcacc aatggccatc ggcgagtgga cgacatactg gacgatctca
     1621 gaatggatca tccggctagc tatgttgccg gtgatcgtca tgagaaagga acgtccggcg
     1681 acatgccttg cgtggctgac agtggtgttc tttgccccac tgccaggttt acttctctac
     1741 ctgctggtgg gcgaaaaccg gcttggccgc cgccgtttgg aaatccgcaa aagccgctgg
     1801 gcaaaactgg agagtgtcaa cgctccaccc gtgggggctc actatcagtc cgatagcagc
     1861 gaactttggg gggagccagc acgagaggac gccgacgaag ctgatgtcgc cgacgattct
     1921 ctccagaccg ggacgatggg aaaggggggc cttgatcccg aactccagcc actcagcgac
     1981 cttgcctttc gcttgacggg ttggcctgta acgactgaaa accaattgtc atttctttgt
     2041 gatccacttt tgacgataga ccgcctcgtg gcggagatcg attcggccaa cgaccacgtg
     2101 catcttttgt actttatttt ccgggacgat gaaattggac ggcgggtggc cgacgccttg
     2161 gtgcgggcag cacagcgggg cgtccaatgt cgagtgctgg ttgacgccgt cggttcgcgc
     2221 cccatgctca aacgacttgc cccccgactc cgcggagccg gtgtcgaggt ccaccctgcc
     2281 cttcctgttc gactctaccg aatccctttt gcccgtttgg accttcgtaa ccatcgcaaa
     2341 ttggctatca ttgatggaag ggtggcttac acgggatccc aaaacattgt ggaaccaacc
     2401 tatggccgtc gaaaggcggg gccctggcat gatatcacgg cccgaattgt ggggccgagc
     2461 gtcccccact tacagttact ctttctagag gattggtttc acgagacagg ccagtggctc
     2521 gatgaacccc ggtatttccc cgtcatcagt gagggcggcc aaaccattct gcaggtcctt
     2581 cccagcggtc ccgacatgcc gacggagctc tttcaggacc ttctggtgaa ggccattttc
     2641 ctcgcgcgtc ggcgggttat tttgacgtca ccgtactttg ttcccgacga gccgttgacg
     2701 atggcgttgc gattagcagc ctaccg
//
LOCUS       DAOPTR010000518         2723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_29762_length_2723_cov_3.171289, whole genome shotgun sequence.
ACCESSION   DAOPTR010000518 DAOPTR010000000
VERSION     DAOPTR010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29762_length_2723_cov_3.171289"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="PKI05_10145"
     CDS             <1..870
                     /locus_tag="PKI05_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOA52606.1"
                     /translation="LMGPGDLILHDALSHNSILQGAILSGARRRPFPHNDWRAADRLL
                     ELFRREYRRVLIVIEGVYSMDGDIPDLPHFIEVKKRHKALLMVDEAHSMGVLGETGRG
                     VGEYYHVPREDVDIWMGTLSKSFGSCGGYIAGKKSLVEYLKYTAPGFVFSVGLSPPNA
                     AAALASLRLLKAEPQRVAQLRERAALFLRLAKERGFDTGASHDSPVVPIIIGNSLHCM
                     FLSQALFKRGINVQPIVYPAVEERAARLRFFLTSRHTEEQIRYTIDVMTEEVDKLDPN
                     YRAARLGSMAGHS"
     gene            1049..1885
                     /locus_tag="PKI05_10150"
     CDS             1049..1885
                     /locus_tag="PKI05_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOA52607.1"
                     /translation="MACLSVSETTTFRWSFEEDIHRYRAAGIPAIGVWRQKLSDFGEE
                     KGAELIVEADLRVSHLFWAGGFTGSDGRSYKAAVDDAREAIQTAAELKTRCLIVYSGS
                     RGTHTFNHARRLFRDALKELTPLAQEFDIYLGVEPMHPGCGCEFTFVSDIEEFLRLVD
                     AVGSDRVKLVFDTYHLGLTPDIVAQIPRLVPHIVLVQLGDAKRPPQGEVNRCLLGEGV
                     IPVKAIVDTLLDNGYDGFFDVELIGEDVEHCDYTQLLEHARKAYEQLVGPIGPLAVSQ
                     ES"
     gene            2592..2665
                     /locus_tag="PKI05_10155"
     tRNA            2592..2665
                     /locus_tag="PKI05_10155"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2626..2628,aa:Val,seq:tac)
BASE COUNT          595 a          748 c          773 g          607 t
ORIGIN      
        1 ttgatgggac cgggcgacct gattctccat gacgcgcttt cccacaacag cattcttcaa
       61 ggggcgatcc tctccggtgc ccgtcgccgt cctttccccc acaacgattg gcgggcggct
      121 gaccggctgt tggagctttt ccggcgcgag taccgccgtg tgctgatcgt catcgagggg
      181 gtttatagca tggacgggga catcccggat ttgcctcatt tcatcgaggt caagaaacgg
      241 cacaaggccc ttcttatggt tgatgaggcc cattccatgg gggtcctcgg agagacaggc
      301 cgcggagtcg gcgagtatta ccacgttccc cgcgaggacg tggatatctg gatggggacg
      361 cttagcaagt cctttggtag ttgcgggggc tacatcgccg gaaagaaatc gttggttgaa
      421 tatctcaaat acacagcacc cggtttcgtc tttagcgttg ggctttcgcc tcccaatgcc
      481 gctgccgctt tagcctcact acgcctattg aaggcagagc cgcaacgggt ggcgcagttg
      541 cgagaacggg cggccctgtt cctccgcttg gccaaagaac gcggattcga taccggcgcc
      601 agccatgatt cgcccgtcgt cccgatcatc atcggcaact cgctccactg tatgttcctt
      661 tcgcaggcct tgttcaaacg agggatcaac gtacaaccga ttgtgtatcc ggccgtcgaa
      721 gaacgggccg cccgattgag gttcttcctc acctcacgcc ataccgaaga acaaatccgc
      781 tacacaattg acgtgatgac tgaagaagtg gacaaactcg atcccaacta ccgggccgcc
      841 cgtttgggca gtatggccgg acattcgtag tcgcactcga tatagtcatc cgtggtgacg
      901 cggccacaat ccttggggga tcggccagaa aatattgccg acccctttat ttttctctac
      961 gatttggcca tactggaaac gctttgataa ggcggttctc ggacggttgg aaaatggtca
     1021 cggttggttt agttggaggc aaacacggat ggcctgtttg tcggtctccg agacgaccac
     1081 gtttcgttgg tcctttgagg aagatattca ccgatatcgt gcggcgggca ttcccgccat
     1141 cggtgtttgg cggcaaaaac tctcggattt tggcgaagag aagggggcgg aactgatcgt
     1201 cgaggcagac cttagagtct cccatctctt ctgggcaggt ggttttacgg gcagcgatgg
     1261 ccgctcgtac aaggcagccg tggatgacgc ccgcgaagcc atccaaacgg ccgcagaact
     1321 gaaaacccgt tgtctgatcg tctatagcgg ttcgcgtggg acgcatacct tcaaccatgc
     1381 ccgacgactc tttcgcgacg cccttaaaga gctgacgccg ctagcccagg aatttgacat
     1441 ctacctcggc gtggaaccga tgcatcccgg ctgcgggtgc gaattcacgt tcgtctccga
     1501 catcgaggaa tttcttcgct tagtcgatgc ggtgggctcc gatcgagtga agcttgtgtt
     1561 cgatacctat cacctcggtc tgacgccgga catcgtggcc caaattcctc ggttggtgcc
     1621 acacatcgtt ttggtgcagc taggcgatgc caaacggccg ccgcaaggtg aggtgaatcg
     1681 ttgcctcctg ggtgaaggag tgatccccgt caaagccatt gtcgataccc tcttggacaa
     1741 cggctacgat gggtttttcg atgtggaact gatcggtgag gatgtagaac actgcgatta
     1801 tacgcaactg cttgagcacg ccagaaaggc ctacgaacaa cttgtaggcc ctatcggccc
     1861 attggctgtc tcccaagagt cgtaaccaac gcgccaaaag agagttgcgg ccggtaggcg
     1921 cagggaccta ccccattgag gtggcgcgga tcaaaaatat ggggccccat ttgtgatatg
     1981 ggggtgctgc tctatcgact atgcgtgttg caaagcctgt ggcagcaacc ccaaacgttg
     2041 tcttctcttg ggaacgaatg gatgcattat cctcctgaat tcggaggtgc gatttatcgc
     2101 cttttttagc aactagctct ctttctaatt tggggggacc ggcgacatcc tatgcgactg
     2161 aagagcctat cccgaaaagt ccccctctcc ctctgggaga gggttggggt gagggcaagc
     2221 gaacgtcgga atcgattcag aaaacccgca gcgattcgag ccaccctccc cccgacccct
     2281 ctcccgccga acaggcgagg ggaccttaca ggataggctc taagtacccc ggtcgtaacg
     2341 ccaaccactc gcctcaaacg ggaacaccgc aaaaggtctt cacgccgccg attgacgttt
     2401 tcccattgaa ggcggacaat aaaatacaaa cgtgtcgaag agagtctgac gaaggaatat
     2461 gccgagcacc gagaaaggaa atcacattcc ggatgtgagc gccgtcaact gttaacttcg
     2521 gatcgtcctt tcggggttgt gtcctgaagt gatacgaaat ggagaacggg tgacaaggac
     2581 gtcgcacatt agggcgatta gctcagttgg ttagagcgcc acccttacaa ggtggaagtc
     2641 ggccgttcga gtcggccatc gcccatcagg gtatttgcac tagtcggcaa catagcgcaa
     2701 aacgcggaaa aaacggggtt ttc
//
LOCUS       DAOPTR010000519         2721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_29798_length_2721_cov_2.478995, whole genome shotgun sequence.
ACCESSION   DAOPTR010000519 DAOPTR010000000
VERSION     DAOPTR010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29798_length_2721_cov_2.478995"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            303..2123
                     /locus_tag="PKI05_10160"
     CDS             303..2123
                     /locus_tag="PKI05_10160"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOA52608.1"
                     /translation="MVVWGRSVRLLGYSVLVGVGAGAAAVLFTLALELVEHSALGRIM
                     DYVPPPEHTADPIARFQLPRQWWAVLLVPTLGGLLCGVIVYFLEPASEGHGTDAMIRA
                     FHRLKGVIGPRVPLIKMITSIVTIGTGGSAGREGPMTQIGAGIGSSLAQYLKLGDWDR
                     RILMLAGAAGGLGAVFSTPLGGAMFSVEVLYSGTAIEFSAVAPAFVASVVAYTIFTSF
                     LGQTVLFVLPTAPDPQTGALPCAFHGAHELPFYLALALLCAAVSYIFVNMFYFGRDRI
                     FRLARLPQWLRPALGGLGLGIIVLVGYPHVMAGGYGWIQKAILASPDISLRLAISLVV
                     LKMVATTLTVGSGGSGGLFAPAPPTPPMTGAAFGMACQALFPGSAPPVSATALVGMGG
                     VLAGTFKVPLAALIMVSELTGSHDLLVPMMLVGAVEMVSLPGNVSLCREQVTAFIDSP
                     AHLGDFVVDVFKEMHVRDVCPFSTQPIVLREDTPLPQILRTVADAHQTTFPVVDRDNK
                     LVGVFSLSDLRAALVGDGAGNLVRAIDLAVPVPIVTPDDDLHTVLQIMAEKHLEELPV
                     VMSHAGQQTISLIRRDFILAAYDRRVSLIQTAAASERTPV"
     gene            complement(2367..>2721)
                     /locus_tag="PKI05_10165"
     CDS             complement(2367..>2721)
                     /locus_tag="PKI05_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52609.1"
                     /translation="RWCDLLLSRLSEFGDVSAYAVDPQRWQEFSADFKAIPTEKQRNQ
                     AWQLMRTSLLAAYAVPMFRYHMQLSQTVLVAGYILDCFPQDVLEICQDLNFYRESRFV
                     ARCRRAQELLAEVNV"
BASE COUNT          537 a          740 c          766 g          678 t
ORIGIN      
        1 tggtggtata tactttgttt ttctcggagc tagcatgccc cagcgaggaa aaagtacagg
       61 tcgaggaaat aaagtaatca atcttgtaag tacgtttcca tcacatccta agaataccat
      121 ccacgtctaa taaacagctt tgtgacttgc acaccatgtg tccaaccaac gtttcgaatg
      181 ccgttcggtt tccgtcttcg ccaaacggtg aggaggacgg aggcgggaca aaacatcgct
      241 ttggtcggtt ggtgatgatg ggtcaaagac ttcggcgcgc cttgaacggt ttttcccgtc
      301 acatggtggt ttggggcagg tcggtacgac tgctgggtta tagtgtgctt gttggcgtgg
      361 gcgccggcgc ggcggctgtg ctttttacgc tcgctcttga gcttgttgag catagtgcct
      421 taggcagaat catggactat gttccacctc cggaacacac ggcggatccc atcgcccgct
      481 ttcagcttcc ccggcaatgg tgggcagtac tgttggtccc cacgcttggg ggtttgctat
      541 gcggtgtgat cgtttatttt ctggagcctg cgtcagaggg ccatggcacg gatgccatga
      601 ttcgggcatt tcaccgactg aaaggggtca tcggacctag ggtaccgttg atcaaaatga
      661 ttacgagcat cgtgacaata gggacgggcg gttctgctgg tcgcgaaggt ccgatgacac
      721 agatcggcgc cggtatcggt tcatcactag ctcagtacct aaagttagga gactgggatc
      781 gacgtattct gatgctggcg ggggcagccg gcggattagg ggcagtcttt agcacgccgc
      841 taggtggagc gatgttctcg gtggaagttc tttattccgg gacggccatt gagttttccg
      901 ccgtggcacc agcattcgtg gcttccgtcg tggcttacac tatttttact tcgtttctgg
      961 gacagaccgt cttattcgtc ttaccaactg cccctgatcc ccagaccggg gccttgccgt
     1021 gcgcctttca cggcgcccat gaattgccct tctatctggc gcttgctttg ctctgcgcag
     1081 cagtttccta catatttgtt aacatgtttt actttggtcg agaccgtatc ttccggctcg
     1141 ctcgcttgcc gcagtggctg cgacccgccc tgggaggtct cggcttgggt atcatcgtcc
     1201 ttgtgggcta tcctcacgtc atggcaggtg gttacggatg gatccagaag gccatcctcg
     1261 caagccccga catatccctt cgtttggcaa tcagccttgt cgttctgaag atggtggcaa
     1321 cgactcttac cgtggggtcg ggaggaagtg gcggtttgtt cgctcctgcc ccccccacac
     1381 cgccaatgac aggagcggcc tttggaatgg cttgtcaagc actgtttcca ggatcagcac
     1441 cccctgtttc agccacggca cttgtgggga tgggcggtgt cctggcaggc acattcaaag
     1501 ttcctttagc agccctgatt atggtttcgg agctcacggg ttcccacgat ttgctcgtgc
     1561 caatgatgct ggtgggtgct gtggaaatgg tctctcttcc cggaaacgtt tccctttgcc
     1621 gggagcaagt cacagccttc atcgatagcc cggcccattt aggggacttt gtcgttgacg
     1681 tgttcaaaga gatgcacgtg cgtgacgtct gccctttcag cacacaaccg atcgtcctgc
     1741 gagaagacac accgttgccg cagatccttc gtactgtggc cgacgcgcat caaacgacgt
     1801 ttcctgtcgt cgatcgggac aacaagcttg tgggggtctt ttcattgagt gatcttcgcg
     1861 cggcgctcgt gggcgatgga gcaggcaacc tggtgcgggc gatcgacctc gcggtgccgg
     1921 tgcccattgt cacccctgac gatgaccttc acacggtgct ccaaattatg gccgaaaaac
     1981 acctcgaaga actgcccgtc gttatgtcgc atgcggggca gcagacaatt agcctcattc
     2041 gtcgtgattt catcttggcc gcctacgacc ggcgggtctc tttgattcaa acggccgctg
     2101 cttccgaacg tacccctgta tgaatatggt tgcgcagcga cgggagtcta ggttttcggc
     2161 tctttcataa caacccaggt cctccgcaca atggcgacca attatgaggc ttgatcgcgc
     2221 ttccaccacg tgtttcgatc cctcgaccta ccaccaacaa taactcgctc accagggtaa
     2281 cccccaatga tcactgatgg cggtgtttct gaataggagc agggatggcc atttgcggtg
     2341 tcaggctcga aaacgttgtg gcccagtcag acgttcactt ctgccagcaa ctcctgggcg
     2401 cgacggcatc gggccacaaa acgagactca cggtagaagt tcaaatcctg acatatctca
     2461 aggacgtctt gagggaagca atccaaaatg tatcccgcca caaggactgt ctgcgacaat
     2521 tgcatgtgat accggaacat gggcacggca tatgcggcca agagcgatgt ccgcatcaat
     2581 tgccaggctt ggttgcgttg cttttccgta ggaatggcct taaagtccgc gctgaactcc
     2641 tgccatcgtt gtgggtcaac ggcgtacgcc gagacatcac cgaattcact gaggcgactg
     2701 agaaggaggt cacaccaacg g
//
LOCUS       DAOPTR010000520         2720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_29809_length_2720_cov_3.508818, whole genome shotgun sequence.
ACCESSION   DAOPTR010000520 DAOPTR010000000
VERSION     DAOPTR010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29809_length_2720_cov_3.508818"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2720
                     /locus_tag="PKI05_10170"
     CDS             <1..>2720
                     /locus_tag="PKI05_10170"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOA52610.1"
                     /translation="ALPNVNQADLMGVRPYEISVEISEKTLREYGLTLQKVAQVIRQE
                     NIEVPGGTLRAHSQEVLLRGKNKGMTGKELASIPLVTQPDGRILTLGDLATIQDGFAD
                     TTAVSRINGRPGMAVVVTSTSTEDLLKTTKDVHEYVAQKKLPPGYELVTWGDISVYVR
                     DRLNLLAKNGLQGLIIVFVLLAVFLEIHLAFWVALGIPVAMLGTAIIMQWSDQTLNMM
                     SVFAFLMALGMLVDDAIVIGENVYVHRQMGKGYVQAAIDGTYEVLPSVTAAVATTVIA
                     FAPLFFVPGIMGKFIWLLPFGVVTMLLVSLMEAVFILPCHLAHDPSKWERQFGGTAEE
                     NSFAGSDAPQPVRSGRRWLGIIWRVSLTFLLVGVLLILSRGEIPWEMSWPVWVICVII
                     VALITFARALPRIEGFTNLINRYADIALQTFIKRVYMPSLKLALRFAPVTLALAIALL
                     IASLGLVVSGYVPFVPFPKLDAESIEAVVYYPAGTPEDVTNRATLAIEQAIRDVDEEY
                     RRQGRPLVKTIYRVVGEVTVGALRVGNELPTGSHSGKVEVELVEAAVRDLSSEEIVKR
                     WRERSLELGRQFPGAESVTFQSQARGPGGLPIEFRLLAPVHRMAELEEAVEKCKAYLA
                     EIPGVFDIQDDARPGKWEFQIKTTDKAKALGVSLLDLAGTVRAAYYGEEAMRLQRGRH
                     EVKVMVRYPEEERRSLARFDDIRIRAGGIERPITELAEITVARGYSQIHRLNQLRAIA
                     ITADVDEAVVNSQKVITDLKTRFLPDLLSNPRYSDIQISWEGAQESTNESISAMIRGL
                     LVALIAMFALLTLEFRSYFQPIMILIIIPFGLLGAIVGHLIMGLPLTLMSIFGMVALT
                     GVVVNDSIVLVDFINSRVREGKPIREALIESGQRRLRPVLLTSVTTIGGL"
BASE COUNT          566 a          680 c          794 g          680 t
ORIGIN      
        1 tcgcattgcc gaatgttaat caggctgacc tgatgggcgt tcgcccgtac gaaatttcag
       61 tggaaatctc tgaaaagacg ttacgcgagt acggcttgac gctgcaaaag gttgcccaag
      121 tgatccgtca ggagaacatc gaagtgccgg gtggaacgct ccgggcgcac tcacaggaag
      181 tgcttctccg gggcaagaac aaaggcatga cgggcaagga gcttgcaagc attcctctcg
      241 tcacacaacc ggacggacgt attctaacat tgggtgattt ggcaacaatt caagatggtt
      301 ttgccgacac cacggcagtg agccggatca acggacgccc gggaatggct gtcgtcgtta
      361 cgagcacctc gacagaggac ctcctcaaga cgacgaaaga tgtccacgaa tatgtagcgc
      421 agaaaaagtt gcccccgggt tatgaacttg ttacctgggg ggatatttcg gtttatgtcc
      481 gcgatcggct caatcttttg gccaagaatg gtttgcaggg gcttatcatc gtgttcgtgc
      541 tcctcgccgt atttctagaa atccatttgg cattttgggt ggcacttggc atccctgtcg
      601 ccatgctggg gacagccatc atcatgcagt ggagcgatca gaccctgaat atgatgtccg
      661 tgttcgcgtt cctcatggcc ctggggatgc tggtcgacga cgccatcgtc atcggtgaaa
      721 acgtttatgt ccaccgacag atgggcaagg gctatgtgca ggcagcaatt gacggaacat
      781 acgaagtcct gccttccgtg actgctgcgg ttgcgacaac agtgatcgcc tttgccccgt
      841 tgtttttcgt cccgggcatt atgggtaagt ttatttggct cctaccgttt ggggtcgtga
      901 cgatgcttct tgtatcgttg atggaagccg tgtttatatt gccttgccac cttgcccatg
      961 accctagcaa gtgggaacgg cagtttggag gtacggcgga ggaaaactcc ttcgctggat
     1021 cagatgcccc gcaaccggtg cgctcaggcc gacgttggct gggaatcatt tggcgagtca
     1081 gtctcacatt tctcttggta ggggtattgt tgattttgag ccgtggtgaa attccttggg
     1141 agatgagttg gcccgtttgg gtcatttgtg tcattattgt ggccttgatt actttcgccc
     1201 gtgccctgcc gagaattgaa ggctttacga accttatcaa tcgttacgcg gatattgcgt
     1261 tgcagacttt catcaagcgt gtttacatgc ccagtcttaa gttggctttg cgttttgcgc
     1321 cggtgacact ggctctggca attgcactct tgatagcttc actgggcctt gtagtctccg
     1381 gatatgttcc atttgttccc ttcccgaagc tcgatgccga atccattgag gccgtggtct
     1441 attatccggc cggtacaccg gaggatgtca cgaatcgggc gacgctcgcc atcgaacagg
     1501 cgattcgtga tgtggatgaa gaataccgcc gtcagggcag gccgcttgtc aaaacaattt
     1561 accgagtggt gggagaagtg acggtgggcg ctttgcgggt gggcaacgag ctgcccacgg
     1621 gcagtcactc gggcaaggtc gaggtggaac ttgtggaggc ggcggtgcgg gacttaagca
     1681 gcgaagagat tgtcaaacgg tggcgcgaac ggtcgttaga attgggccgt caattcccgg
     1741 gtgctgaaag cgtgacattt cagtcccaag cacgcgggcc tggtggattg cccatcgagt
     1801 ttcggctttt ggcaccggtc catcgaatgg cggaacttga ggaggcggtc gaaaaatgca
     1861 aagcgtatct ggctgagatt ccgggtgtct tcgatatcca agatgatgca cggcctggca
     1921 aatgggaatt tcagataaag acgaccgaca aggctaaggc cttgggggtt tcgctcctcg
     1981 atttggcggg aacggtccgc gcggcatatt acggcgaaga ggcgatgcga cttcaacggg
     2041 gccgtcatga ggttaaagtc atggttcgct atccggaaga ggaacgtcgc tccctagctc
     2101 gctttgatga tatccgaatt agggcagggg ggatcgaacg ccccatcacc gagctggcgg
     2161 aaatcaccgt tgccagaggc tattcgcaga ttcatcgtct caatcaactg cgggccatag
     2221 ctattacggc cgacgtggat gaagccgttg tcaattcgca aaaagtcatt accgatttga
     2281 aaacccggtt ccttcccgac ctcttgtcca atccccgcta ttcggatatc cagatctcct
     2341 gggaaggtgc ccaagagtcg accaacgagt cgatttccgc catgatccgc gggcttttgg
     2401 tggccctaat cgctatgttt gcactcttga cactcgaatt ccgctcgtat tttcagccta
     2461 tcatgattct catcatcatt ccgtttgggc tgctaggtgc gatcgtcgga cacctcataa
     2521 tgggtcttcc gctgaccctc atgagcattt ttggaatggt ggccctcaca ggtgtggtgg
     2581 tcaatgactc cattgtgctg gtggacttta tcaattcgcg ggtccgtgag ggtaaaccga
     2641 ttagggaggc cctcatcgaa tcagggcagc ggcgcttacg tcccgtcctg ttgacctcgg
     2701 tgaccaccat cggtgggctg
//
LOCUS       DAOPTR010000521         2718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_29834_length_2718_cov_4.158843, whole genome shotgun sequence.
ACCESSION   DAOPTR010000521 DAOPTR010000000
VERSION     DAOPTR010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29834_length_2718_cov_4.158843"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PKI05_10175"
     CDS             <1..411
                     /locus_tag="PKI05_10175"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOA52611.1"
                     /translation="EFTRRTHQRVPESEGAVLRCALDSIAMKFRHVLEMCEELIRRRI
                     EVVHVVGGGTKNRLLCQAAADACGRPVLAGPVEATAIGNIMMQAVAAGEVGNIAEARE
                     IVRESFPLDEYTPRDVQAWEDAYSRFLDVINTEI"
     gene            522..1544
                     /locus_tag="PKI05_10180"
     CDS             522..1544
                     /locus_tag="PKI05_10180"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA52612.1"
                     /translation="MVRVGIVGIGFMGMIHYLAYQKLKGVKVVAICEKIPERLAGDWR
                     SIKGNFGPKGRKMDLSGIAKYSEIEELLKDPNVDVVDICLPPALHPQATVAALEAGKH
                     VFCEKPIALTTADADRMVKTAQKKGKMLLIGHVLPFFAAYRFAYDAVTSGRYGACLGG
                     QFTRIISDPLWLPDFYNPNVCGGPAIDLHIHDAHFIRVLFGMPKSVHSVGRMRGEVPE
                     FFNTQFLYDDPRLVVTATSGVIMQQGRPFTNGYEIHLEKATLVFDSWMNMPVTILTED
                     GKVKRPKIPTTDPVDDFAAELAEVVRTIKTGEPSKFLDGQLARDALVLAHRQVESVKK
                     RRAVKV"
     gene            2099..2692
                     /locus_tag="PKI05_10185"
     CDS             2099..2692
                     /locus_tag="PKI05_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52613.1"
                     /translation="MGSAGWGGLSVFAGDTENAATPVVPAAEQLRSILEQGLSLEHQS
                     RWGEALTLYEDALRQFPDDKVLEQRFHEARLHYDLLRRHSDHTYLELVGRLSSSEVFQ
                     IYDEVLLKIHAHYVEAPGWRYLYEQGVHAVAIALTEPRFLAAHRLSISAEATAATARD
                     LPLVVPAQNVQSRMTAIEATTRVANWLRERIGLPPQI"
BASE COUNT          595 a          695 c          786 g          642 t
ORIGIN      
        1 gaattcactc ggcgcacgca tcaacgggta ccggaaagtg aaggggctgt cctgcgatgc
       61 gccctcgaca gcattgccat gaagttccgg cacgttttgg aaatgtgtga ggaattgatc
      121 cggcggcgaa tcgaggtcgt acacgttgtt ggtggtggta ccaagaatcg gttactctgt
      181 caggctgctg cggacgcgtg tggccgaccc gtcttggcag ggccggtgga ggccacggcc
      241 atcggcaaca tcatgatgca ggccgtcgcc gcaggtgaag ttggaaatat cgccgaggca
      301 agagagatcg tccgcgagag tttcccgctg gacgaataca ctccgcgtga tgtccaggct
      361 tgggaggatg cctattctcg ttttctggat gtgatcaaca cagagattta acgatttacc
      421 aatgacgggt tcgtgggaag tctctagggt tcgttagccg aacgaggaga cgccgttttg
      481 gggagttgct tgtgaggctg tcagcatagg agaagtgaga tatggttcgc gtgggaatcg
      541 tcggcatcgg gttcatggga atgatccact atttggccta ccaaaagctc aagggcgtca
      601 aggtagtggc catttgcgag aagataccag aacggctggc cggggactgg cgatccatca
      661 agggcaattt cggccccaag ggacggaaaa tggacctttc cgggattgcc aaatactctg
      721 aaattgagga acttctcaag gacccgaatg tcgatgtggt cgatatatgt ctccctccgg
      781 ccttgcaccc acaagcaact gtggccgctt tagaggcggg caaacacgtg ttctgtgaaa
      841 aacccatcgc actgacgacg gccgatgccg accgaatggt gaagacggcc cagaaaaagg
      901 ggaaaatgct ccttattggc cacgttttgc cattcttcgc ggcctatcga tttgcctatg
      961 atgctgtgac gagtgggcgc tacggggcgt gccttggcgg tcagttcacg cggatcattt
     1021 ccgatcctct ttggttgccc gatttctata atcccaacgt ctgcggcggt ccggcgatcg
     1081 atttgcacat ccatgatgcc catttcattc gcgtgctatt tggcatgcca aagtctgtcc
     1141 acagtgtggg gcgaatgcgg ggtgaagtgc cggagttctt taacacccag ttcctctatg
     1201 atgacccacg gttggtagtg acggccacca gtggagtcat catgcaacag ggaaggccgt
     1261 tcaccaatgg gtacgaaatc catctcgaaa aggcgacact tgtcttcgat tcctggatga
     1321 atatgccggt caccatcctc acggaggatg ggaaggttaa acgccccaag ataccgacaa
     1381 ctgatccggt tgatgatttt gccgcagagt tggcggaagt ggtacggacg atcaagaccg
     1441 gagaaccgtc aaagtttctc gatgggcaat tggcgcggga cgctctggtc ctggcccatc
     1501 gacaggtcga gtccgtgaaa aagcggcgag ctgttaaagt ctgaagccgt ttaagcgtga
     1561 tctcgcttga aacatattga aggtttcact cttaaagtgc gcatcccgct ctcctgcggg
     1621 atgtttttgt aaatggcagt ctcgccacaa ttggtcattg cggttttcgg ggcattcttt
     1681 ctgtggccct caattccggc aggccaacag tcggttattc caatcgtatc gataaaacat
     1741 tgacaaaact cggttagtgc ttttgcatgg tgccgattcg taagatatac tcattcgaac
     1801 ccgttaatcg gatgacgaat cccatcgggt ttcgaatggt gggggcgttt ttctccgcat
     1861 aagcggaaca gttgcactgg acgaaatagg tcaacaagtc gctatttcta gccatctccc
     1921 caattgatta ggtcacaaga cgtccgtccc ttaaccctgg attaggcccg ctatgcaaca
     1981 aggacgcgac agtcggctgc aaagaatcga catccgcgca cctcgtcacc agcgacgtgg
     2041 cggtacttgg ggcacctcag ttgcaataaa ggcaatcgtt tccctttttg ttttttgtgt
     2101 gggttctgcg ggctggggcg ggcttagtgt ttttgccggg gataccgaaa acgccgctac
     2161 tccggttgtt cccgcagcgg agcagctcag gtccatcctg gaacagggct taagcctcga
     2221 gcaccaatca cgttgggggg aggccctgac tctttacgag gatgccctcc ggcaattccc
     2281 ggatgacaag gtcttggagc aacggtttca tgaagcccgg ctgcattacg atcttttgcg
     2341 gcgtcacagc gaccatacgt atttggaact ggtaggtcgt ctcagttctt ctgaagtgtt
     2401 tcagatctac gacgaggtcc tgctcaaaat ccacgcccat tatgtcgaag caccgggatg
     2461 gcgctatcta tacgaacaag gcgtccacgc ggtcgcgatt gcattgacgg agccgagatt
     2521 tttggccgcc catcggcttt ccatttctgc ggaggcgact gcggcaacgg cccgagattt
     2581 acctctggtt gttcctgccc aaaatgtcca atcaaggatg acggccatcg aggccacgac
     2641 tcgggtggcg aattggttgc gcgaacgaat cggcttgcca ccgcaaatct gacggactcc
     2701 ggcccgtgcc agctcgcg
//
LOCUS       DAOPTR010000522         2707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_29982_length_2707_cov_3.046380, whole genome shotgun sequence.
ACCESSION   DAOPTR010000522 DAOPTR010000000
VERSION     DAOPTR010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29982_length_2707_cov_3.046380"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="PKI05_10190"
     CDS             <1..931
                     /locus_tag="PKI05_10190"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOA52614.1"
                     /translation="IQIGTSADLMPGESVIALGNAYGYEHTATRGIISALHRAVQVSD
                     SQYYDDLIQTDASINPGNSGGPLLNINGEMIGVNVAVRAGAQGIGFAIPVDRVVEAAA
                     ELLAQINDQKLYYGVRFRSAPPTGPLTVSEVDPNSPAAAAGLQPGDVITEVANRKVRN
                     ALDFQRALLEKSADDVLPVVVTRNGEGNQQLMLTLSKPKTATLDDEIWMMLGLRLEPI
                     PEETFRQKFQTRYRGGLLITEVRSGSPAAAQGLRPGDVLVGMHLWETISLDNVEYIIH
                     RPDFASFLPLKFFLLRGNETYYGYFSVALRSEQ"
     gene            complement(1038..>2707)
                     /locus_tag="PKI05_10195"
     CDS             complement(1038..>2707)
                     /locus_tag="PKI05_10195"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA52615.1"
                     /translation="PCRSTLITGMYSVTLGSQHLRSPIRLPKEVHCFPELLRKAGYYC
                     TNNVKQDYNFECPPGVWDESSPRAHWRNRPDGQPFFAVFNTMVTHQSRVRMSDDEFAK
                     EIAALEPVTRHDPATVPLPPYYPDTPGIRKEMARYYDMISAADRWVGKHLQELADAGL
                     KEDTIVFVFGDNGAGIPRHKRALYDTGVRVPLLIWFPKKFAHLAPAQPGSVVSEIVSF
                     VDFAPTILRLAGVEPPELMQGQAFLGTHGGSRLHAYLFRDRVDEVFELSRGVRDQQFK
                     YIRNFMPHRPYMEISEYCEPAQMVRDLRRLAAEGQLSGPAALYLRETKPIEELYDLKN
                     DPYELHNLAADPKYAQILQRMRQELRTYVLSLRDTSFLLESDMFRRAGDQTIWEMAQD
                     DEKYPLARIYAAADAVGRPEKSYQDFEAFFADADPAVRYWGVIGLMSMTELPQRAVSR
                     LYELLDDSSPEVRIASAEALCRLQKPEKAVQVLGKELRSSDPYVRLLAASTLAVINGH
                     AAGARDPILEIIGEKGTGDMWMFTQWALARCCRQLNWPIPAHVPTQY"
BASE COUNT          602 a          785 c          726 g          594 t
ORIGIN      
        1 catccaaatc gggacgtctg ccgacctcat gcctggcgag tccgtaattg ccctgggaaa
       61 cgcctatggc tatgagcata ccgccacacg tgggatcatt agcgctcttc accgggcagt
      121 gcaagtcagt gactctcagt actatgacga cttgatccaa acggacgcaa gcatcaatcc
      181 cggcaactcg ggtggacccc tgcttaacat caacggcgag atgatcgggg tcaacgtcgc
      241 cgttcgtgcc ggggcgcagg gaattggttt tgccattccc gtggatcggg ttgtcgaggc
      301 cgctgccgag ctccttgccc aaatcaacga tcaaaagctc tactacggtg tgcggttccg
      361 ctccgctcca ccaactggcc cattgaccgt gtctgaagtc gatcccaaca gcccggcagc
      421 cgccgcagga cttcaacccg gggatgtcat caccgaagtc gccaaccgca aggtccggaa
      481 cgccctggac tttcaacgag ccttgctaga aaagtctgcc gacgacgtcc taccggtcgt
      541 agtgacgcga aatggcgagg gcaaccagca acttatgctc acactgtcca aacccaagac
      601 cgcgactttg gacgacgaga tctggatgat gctcggctta cgtctggaac cgatcccgga
      661 ggaaaccttc cgccaaaaat ttcagactcg ctaccggggc ggccttctta tcaccgaggt
      721 gcgcagtggc agtcccgcgg cagctcaagg tctccgtccc ggcgatgtct tggtgggcat
      781 gcacctctgg gaaacaatct ccttggataa cgtagagtac atcattcatc gccccgactt
      841 cgccagcttt cttccgctca agttctttct ccttcgtgga aacgaaacct actacggcta
      901 cttctcggtc gccctccgct ccgagcagta acagtattca ggaattgtcg ctcgcgttac
      961 taaggctcct ttcagcgatt agtcgcccag ccggcatctc ggacttttca cggttggaaa
     1021 aacataccgg ttcaccatca atactgggtg ggaacatgcg ccggaattgg ccagtttaat
     1081 tgtcggcagc atcgtgccaa ggcccattgg gtaaacatcc acatgtcgcc ggttcctttt
     1141 tcaccaatga tttccagaat ggggtcgcgg gcaccggccg catggccatt gatgacggca
     1201 agtgttgaag cggcaagcaa gcgaacataa ggatctgacg accgtaattc tttgcctaat
     1261 acctggacag ccttttccgg tttttgaagc cggcacaggg cttcggccga agcgattctc
     1321 acttcaggtg aactgtcgtc gaggagttcg tagagccgag aaacagctct ttgaggcagc
     1381 tctgtcatgg acattaagcc aatcaccccc cagtatctaa ccgcaggatc agcatctgcg
     1441 aaaaaggctt caaaatcttg gtagctcttt tccggacgtc cgaccgcgtc ggcagccgcg
     1501 tagattcgag ccagtggata cttctcatcg tcctgagcca tttcccagat agtctgatcg
     1561 ccggcccgac ggaacatatc actctctagc aaaaagcttg tgtcgcggag agataagacg
     1621 taggttcgca actcctgccg cattcgctgt agaatctgtg cgtactttgg atcggccgcc
     1681 agattatgca gttcgtaggg gtcgttcttc aaatcgtaaa gttcttcaat cggtttggtt
     1741 tctcgcaagt acaaggcggc aggacctgaa agctggccct cggcagcgag gcggcggaga
     1801 tcacgaacca tctgcgccgg ttcgcaatac tcactgatct ccatataggg acgatgcggc
     1861 atgaagttgc gaatgtattt gaattgctga tcgcggaccc cacggctcaa ctcgaagact
     1921 tcatcaacac gatcgcggaa cagataggcg tggagtcttg agcccccatg tgtcccgaga
     1981 aaggcttgcc cctgcatcag ttccggtggt tctactcctg ctaaacgaag gatcgtcgga
     2041 gcgaagtcca caaagctcac gatctccgag acaacgctgc ccggctgcgc cggggccaaa
     2101 tgagcaaact tcttcggaaa ccagatgaga agcgggacgc ggaccccggt gtcgtacaag
     2161 gcacgcttat gccgcggtat gcccgcccca ttgtccccga acacaaaaac aatggtgtct
     2221 tctttcagac cggcatccgc tagctcttgc aggtgcttcc cgacccatct gtcagcggca
     2281 cttatcatgt cgtagtatcg ggccatctct ttacggatgc ccggcgtatc ggggtaatac
     2341 ggtggtaacg gcaccgtcgc ggggtcatgc cgtgttacgg gttccaatgc cgcgatctcc
     2401 ttggcgaact cgtcatcact catccgaacc cggctttggt gggtgaccat cgtattaaaa
     2461 acggcaaaaa atggctgacc gtctggccga ttccgccagt gagcccgcgg actggattcg
     2521 tcccaaacgc caggtgggca ttcaaagttg tagtcctgtt tgacgttgtt cgtacagtaa
     2581 tagccggcct tacgcaacaa ttcggggaaa cagtgaacct ccttcggcaa acgaataggc
     2641 gaccggaggt gttgcgaccc gagcgtgact gagtacatcc cggtgattag ggtcgaacgg
     2701 catggtg
//
LOCUS       DAOPTR010000523         2706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_29993_length_2706_cov_3.427386, whole genome shotgun sequence.
ACCESSION   DAOPTR010000523 DAOPTR010000000
VERSION     DAOPTR010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29993_length_2706_cov_3.427386"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="PKI05_10200"
     CDS             <1..595
                     /locus_tag="PKI05_10200"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HOA52616.1"
                     /translation="IGYGAGSRDIPGRANVLRLFVGKSLVPQGAMPGVETDRVWMVQT
                     NWDDASGEVIGYVVEQLWKLDVLDVFVTPIQMKKNRPAVQLTILCPGQLIPQVEQVLF
                     RETTTLGIRFWPVDRHILARRPHVVQTPYGPVQGKVSFLPDGEARFSPEFEACRKLAA
                     EKNLPLRVVYEAAEGAFRENSANHGQTAETPPKEKTP"
     gene            592..1308
                     /locus_tag="PKI05_10205"
     CDS             592..1308
                     /locus_tag="PKI05_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52617.1"
                     /translation="MTYVLLANGWTNPSVWLVLAAIILIWFSLSRAYRRRPAPRDQES
                     EFDNPEERLRSSSLGRALSSSLEQWEVRMHELTRELEGRINSKLGLLEQLIQEADRAA
                     ARLESALRTAESIRKTQAAGMLPASAHPAIEINPPAGTAEKDQTAGNVNPHIPQPQVT
                     QANLQDGITAEAPASVSSSPSTGELPDKGHPRRSVEEIYTLADYGYSPEEIAQRTGTP
                     IGEVQLILSLRKSRNSAEAN"
     gene            1369..2478
                     /locus_tag="PKI05_10210"
     CDS             1369..2478
                     /locus_tag="PKI05_10210"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOA52618.1"
                     /translation="MAAKRNRRNFLEHFVRGEILDDSPSLPSAESSQSHRPTVSKDRP
                     QGGYHLRLSRPAMGSEFQVVLNLGQYEHAVEAALEALDLLEPLENMMSFFRPAGDLGR
                     INALAATVEVPVPDALWDLLVWCRALWEETEGAFDVTAAALWQTWGFARREGRLPSES
                     EIAQARQACGWHNVQLNERDHTIRFLQSGVGLNLGSVGKGYALDRMAERLLSNGIADF
                     VLHGGLSSVLARGRSWEIDAATGQASPGWPIGLAHPLKKGAQVGRMWICDRALGTSGT
                     VRQFFYHKGKRYGHIIDPRTGYPVENALSVTVFAPTAQEADALGTAFFLLGPEKAKSY
                     CQNHPEVGGIFIISPDRLQATIESVGDSPEVLEPS"
BASE COUNT          551 a          779 c          802 g          574 t
ORIGIN      
        1 gatcggttac ggagccggtt cgcgggatat tcctggtcgg gccaacgttt tgcggctttt
       61 tgtgggtaaa tccttagtac cacaaggagc gatgccaggg gttgagacgg accgcgtgtg
      121 gatggtccag accaattggg acgatgcctc tggcgaggtg atcggctacg ttgtggaaca
      181 gctttggaag ctcgatgtgc tagatgtttt cgtcacgccc attcagatga agaagaaccg
      241 tccggccgtg caattgacaa tcctctgccc tggtcagctc atcccgcagg tggaacaggt
      301 ccttttccga gaaacgacga cactgggcat ccgcttttgg ccggtcgatc gccacatttt
      361 ggcccggcga cctcacgtcg tgcaaacacc ctatggcccg gtccagggca aggtcagctt
      421 tttgccggac ggcgaagccc gtttttcgcc ggagtttgag gcctgtcgga aacttgcggc
      481 ggaaaagaac ctcccgctgc gtgtcgtcta cgaagctgcg gaaggggcct tccgtgaaaa
      541 tagtgcgaac cacgggcaga cggccgaaac tccgcccaag gagaaaactc catgacctac
      601 gtattgctcg ccaatggttg gaccaaccca tctgtttggc tcgtactggc ggcgatcatt
      661 ctcatctggt tttccctttc ccgcgcttat cgacgacggc ctgcccctcg tgaccaggag
      721 tccgaatttg acaacccaga agaacgcctg cgtagcagct cgctgggtcg ggccttatct
      781 agctccttgg agcagtggga ggttcggatg cacgagctaa ccagagagct tgaagggcga
      841 atcaacagta agctcggact ccttgaacaa ctcatccaag aggcagatcg tgcggccgcc
      901 cggctggagt cggccctccg caccgccgag tccatccgca agacacaggc cgccgggatg
      961 ttgccggcct cggctcatcc cgccatagaa attaaccccc cggccggcac cgcggagaaa
     1021 gaccaaacgg caggtaacgt caacccccac atccctcaac ctcaggttac ccaggcgaat
     1081 ttgcaggacg gaataaccgc cgaggccccc gcaagcgttt cctcatcgcc tagcactggt
     1141 gagttgcccg acaagggcca tccacggcgg tccgtggagg aaatctatac cctggccgac
     1201 tacggttact cccctgagga aatcgcccag cgaacgggaa cgcccatcgg cgaggtacaa
     1261 ttgatattaa gcctgcggaa gagccgcaac tcggctgagg cgaattaaag ctgtcgtctc
     1321 gcttgcgcgc agtggacaca tctgaccttc ttatattgca tttgattcat ggctgccaaa
     1381 agaaaccgcc gcaatttttt ggaacatttc gttcgtggtg agatcctcga tgattcaccg
     1441 tcgctgccct cggcggaatc gtctcagagc catcggccca ccgtatcgaa ggaccggcct
     1501 cagggtgggt atcatttgag gctatctcgg ccggcgatgg gtagcgagtt tcaagtggtc
     1561 ctcaatctgg ggcagtatga gcatgctgtc gaggccgccc tggaggccct cgatttattg
     1621 gaaccgcttg agaatatgat gtctttcttt cggccggccg gcgatttagg tcgaatcaac
     1681 gccttggcgg caacggtgga agtgccggtc cccgacgcct tgtgggattt actcgtttgg
     1741 tgtcgagcgt tatgggagga aacagaaggt gccttcgatg ttacggcagc cgccttgtgg
     1801 cagacctggg gatttgcccg ccgcgaaggg aggcttcctt ccgaatcgga aattgctcaa
     1861 gcccgtcagg cctgtggttg gcacaacgtt cagctcaacg agcgagacca cacaatccga
     1921 tttcttcagt cgggcgtggg gttaaacttg ggaagcgtcg gtaaaggtta tgccctcgat
     1981 cgcatggccg aacggctcct ctccaacggt attgcagatt ttgtcctcca cggcggctta
     2041 agcagtgtct tggccagggg gcgttcgtgg gagattgatg cggcaaccgg tcaagcctca
     2101 ccgggctggc cgatcggttt ggcccatccc ctgaaaaaag gtgctcaagt gggtcgcatg
     2161 tggatctgcg atcgggcact ggggacgtcg ggaacagtcc gtcagttttt ctatcacaaa
     2221 gggaaacgat acggacacat cattgatccg cggactggtt accccgtgga gaacgccttg
     2281 agcgtcacgg tctttgcccc gacagcccag gaagccgacg ccctgggcac ggccttcttt
     2341 ctactgggac ctgagaaagc caaatcttac tgccaaaacc acccagaagt gggagggatc
     2401 ttcatcatct ctcccgatag gttgcaggcg actatcgagt cggtcgggga ttccccggaa
     2461 gttcttgagc cgtcgtagga ggctcaagct ggtagcagta tctctgatgg gaacctatcc
     2521 cataatctcc cctcgcccgt tcggcgggag aggggccggg gggggacggt ggcgcgaatc
     2581 gaggtcggtt ttctcgatcg acttcctcgg tcgcttcccc tcaccttaac cccctcccga
     2641 agggagggaa ctttgggata ggctctaaat gacggacgcg ggggaccgcg tccctccctg
     2701 ccctca
//
LOCUS       DAOPTR010000524         2705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_30012_length_2705_cov_3.673208, whole genome shotgun sequence.
ACCESSION   DAOPTR010000524 DAOPTR010000000
VERSION     DAOPTR010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30012_length_2705_cov_3.673208"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PKI05_10215"
     CDS             <1..229
                     /locus_tag="PKI05_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52619.1"
                     /translation="FGLMFTTVIALILVPTVYLMYSWVMPVSRKDEEEIEESWKKEHP
                     PAEKEESYVDTIPKVPEGALPEPFLMESPQD"
     gene            485..1507
                     /locus_tag="PKI05_10220"
     CDS             485..1507
                     /locus_tag="PKI05_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HOA52620.1"
                     /translation="MNLREERYSRFALVREMMETPEIIAQFDPAVVADAVPIVQEVKN
                     LFLTGEGSSRIFPAKNLIYENLKRGGVWHIATDGARQAHEYDLKQFAVFAASNSGKTK
                     EVISLFTKLAQEGHPRRLGLTANYGVKLEEVATRCYHLKCGSENAVAATKSVVEQALF
                     YRAMLACQENDSSFWRLKEKAAAAAREVLEMPIPQSLVDRIAQAPLVYFAGRNNGVAE
                     ELTLKTNEITRKKSDFLEGTYAVHGIEEVMDANEVMIVVEPFAAEAEKFHQTLVERVG
                     LTIVAISAEETMFPTIRIPHVPGYETILQLLAGWNLLVEVGVTMGIDLDKPVRARKIG
                     NEYLPG"
     gene            1658..2482
                     /locus_tag="PKI05_10225"
     CDS             1658..2482
                     /locus_tag="PKI05_10225"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOA52621.1"
                     /translation="MQSGVKYMRRLDDRVMASEEAADVFDQWEVGQERWMFVSPHDDD
                     AVIGAGLTIQVGMAAGAEVYVVITTDGRMGYCRIPQRRTITRIRSEEAREAYRMLGLP
                     PERLRCLGFPDCNLNAYRGRHFTTIGDPTEIEGASGLQNAFPYVLRQIRPTRVFLPTP
                     TDLHPDHRIVYEEMLISLFHAQGSIWPELGPPIVRIPVVYEFATYCDFPEPPQIRLVT
                     PVAFLERKLEAIQCFASQEQIEVLVKVQRDVGPVEYLRQVAFNFYSPEKYAALFEG"
BASE COUNT          622 a          662 c          799 g          622 t
ORIGIN      
        1 gtttggcctc atgtttacca cggtgattgc cttgattctg gtcccgacgg tgtaccttat
       61 gtacagttgg gtcatgcccg tatcgagaaa agacgaggaa gaaatcgagg agtcttggaa
      121 aaaggagcac cctcctgcag aaaaggagga gtcttacgta gatactatcc ccaaagtccc
      181 cgaaggtgcc ttgccggaac cttttctcat ggaatctcct caggattgaa ccgtcgcggc
      241 tgcggccgtt tgggtggccg ccccctttgt cttcagttag actcgacgga cgacttgctc
      301 ccacgggtgg gtctcgcagg tcgggatcga cgtcttataa tgggaacatg aatcgttaag
      361 gcagtgtcgg agagttttga aggagcgagg atagatttcg ttcgaactcg gcggtcttgt
      421 tggtcttgca tctaagggaa gcgggtgcca aaagagcccc ttttatacgc ctaggagttt
      481 cccaatgaat ctgcgcgaag aacgttatag ccgctttgct ctggttcggg agatgatgga
      541 aaccccggaa atcatcgctc agtttgatcc tgcggtcgtg gccgatgccg tgccaattgt
      601 gcaggaggtc aagaacctct ttttgacggg cgagggttcc agccggatct ttcccgcgaa
      661 gaacctgatt tacgaaaacc tgaagcgtgg cggtgtttgg cacattgcaa cggatggtgc
      721 ccgccaggca catgagtatg acctaaaaca gtttgccgtt tttgctgcaa gtaatagcgg
      781 taagacaaaa gaagtcatct ccctattcac gaagctcgct caagaagggc atccccgtcg
      841 gcttggtttg accgccaact acggcgtgaa actggaggaa gtcgcaacgc ggtgttacca
      901 cctgaagtgt ggctcggaaa atgccgtggc ggcgaccaag agcgtggtcg agcaagcact
      961 gttttatcgg gcgatgctgg cctgccaaga aaacgattct tctttctggc ggttaaaaga
     1021 gaaggctgcc gcggcggcac gcgaggttct ggaaatgcca ataccgcagt cgcttgttga
     1081 tcgcattgcc caggctccat tggtctattt tgctggccgc aataacggcg tcgccgagga
     1141 attgacactc aagactaatg agatcacgcg aaagaagagt gatttcctcg agggcactta
     1201 tgccgtccat ggaattgaag aagtcatgga tgccaacgag gtcatgattg tggtggagcc
     1261 attcgcggcg gaggccgaga agtttcatca gaccctcgtc gagcgagttg gccttacgat
     1321 cgtggcgatt tcggcagaag agactatgtt cccgacgatc cggataccgc atgtaccggg
     1381 ctacgagacg atcctgcaac ttttagcagg ttggaacctt ctcgtcgaag tgggcgtgac
     1441 catgggcatt gacctcgata agcctgtgcg agctcggaag atcggaaacg agtacttgcc
     1501 tggatgatcg ccctcagatg cggcgggttt tttaagtccc gtttgagcag ccttccaagg
     1561 agaggtaacc tgggactgag aacttggagc aataggagac gcgtaatcat ttagccattg
     1621 gcttgggtct caagtatcaa atccagaggg aactcgcatg caatctggtg tgaaatacat
     1681 gcgtcggctc gatgatcgag tgatggcgtc agaagaggct gccgacgttt ttgaccagtg
     1741 ggaagtcggg caggaacgct ggatgtttgt ctcacctcac gacgatgatg cggtgatcgg
     1801 agcaggcctg acgatccaag tagggatggc tgccggagct gaggtctatg ttgtgattac
     1861 cacagacggc cggatgggct attgccgcat tccgcagcgt cgaacaatca cgcggattcg
     1921 cagtgaggaa gcgcgagagg cctaccggat gttagggctg cctcctgagc ggttacgatg
     1981 cctcggattt cccgattgca acctcaacgc ctatcgggga cggcatttca caacgattgg
     2041 tgatccaact gaaattgaag gtgccagtgg gctgcaaaac gcctttccct atgtgttgag
     2101 gcagattagg ccgacgcgag tgttcttgcc cacccctacg gacctccatc ccgaccaccg
     2161 cattgtctat gaggaaatgc tcatcagttt gtttcacgcc caagggagta tctggccgga
     2221 gttgggtcca cccatcgtaa gaattcccgt ggtatatgag ttcgctacgt attgcgattt
     2281 ccctgagcct ccccagattc gcctggtgac gccagtcgcg tttctcgagc gtaagctgga
     2341 ggccatccag tgttttgcga gtcaagaaca gatagaggtg ttggtaaagg ttcagcggga
     2401 cgttggaccg gtggaatacc tgcgccaagt tgctttcaat ttctattccc cggaaaaata
     2461 cgcagcttta tttgagggct gaaagtgggt agtgcaacga agagaagagg tttgggtaat
     2521 ataatctgcc agtgacggga ttttacagga ccctctatta agagcctatc cgaacaagtc
     2581 cccctctccc tccgggagag ggttggggtg agggcaagcg aaaataggaa tcgatggaga
     2641 aaaccggcat cgattcgagc caccctcacc cccgacccct ctcccgccga acgggcgagg
     2701 ggaga
//
LOCUS       DAOPTR010000525         2695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_30140_length_2695_cov_3.625000, whole genome shotgun sequence.
ACCESSION   DAOPTR010000525 DAOPTR010000000
VERSION     DAOPTR010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30140_length_2695_cov_3.625000"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /locus_tag="PKI05_10230"
     CDS             complement(<1..1303)
                     /locus_tag="PKI05_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52622.1"
                     /translation="MSRKTFILCRQNAGNLVAIMAVVLLGMGMGDRATAEPIAGGVSA
                     ERAAYWAKHLPPKPVGVGAPISQREIWDVLAKDKAFRQVVAEAEEELSRPIAEVPDEL
                     YLDFSRTGNRRRYESRMSARRGRLGNLALAECLENRGRFLAELEKTIAAICQEKTWVM
                     PAHDGRLDNFYGRTVEIDLGAASTAWNMATIDYWLAEKLSPECRRLIASELDRRIFTP
                     FEQAVNTGNPRLWWITGTNNWNAVCLAGVVGAALENIPSPERRGFFVAAAEKYIQYFL
                     QGFTPDGYCSEGIGYWNYGFGHFTLLGETLVQATGGQLDFFALPGVPQIALFGYRMEV
                     APNIYPAFADCSVGSRPDSVLSAYLSRRFDLGLKELENRYLGVGAGPSSFLPEVAIYA
                     MPNSLTNRTGQAAARRHDIRDWFADAGILICRPADLSPDKLA"
     gene            complement(1379..>2695)
                     /locus_tag="PKI05_10235"
     CDS             complement(1379..>2695)
                     /locus_tag="PKI05_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOA52623.1"
                     /translation="ISVANLREKDVARMENLKEMMAGGEIVDLIPSFPALTCPVQANM
                     TTGRPPKRHGIIANGIYWRDRDEVEMWTSPNDCIEVPQLWDLLYHHERGLTSAIWFPQ
                     HAKEAGADFICTPAPIHNPDGSETMWCYTRPENLYGELRQKLGDFPLHRYWGPLANIE
                     STRWIVESAIELAKAARPNLFYIYLPHLDYAAQKDGPDSVAAGKAVLELDEVLGQLMN
                     GLRDAYDGDPCWLIAGEYAITPVERVIYPNRILREAGLLSVREERDGEYLDTTSAAWA
                     LADHQIAHVFVRDGEPEIIDRVMRLFQGLPGIDEVLAGEQRSKYHMDHPRSGEVILTA
                     QPDTWFAYYYWFEDHKAPPFARKVDIHRKPGYDPVELFFDPATRGIPLKPELVHGSHG
                     APVRERFQRTVAISSRPGVFPREPVTDTDIFHVVLKHFGMTMDGLA"
BASE COUNT          611 a          812 c          728 g          544 t
ORIGIN      
        1 cggccagttt gtcgggcgat agatccgcgg ggcgacaaat gaggatgccg gcgtcggcaa
       61 accaatcgcg gatatcatgc cgccgggcgg ccgcttgtcc tgtgcggttg gtcagcgagt
      121 tcggcatggc atagatcgct acctcaggca ggaaactact cggcccagca ccgacaccaa
      181 ggtaacgatt ctccagctcc ttcaaaccga ggtcgaagcg gcggctaaga tacgcgctta
      241 ggacgctatc tggccttgag ccaacgctgc aatcggcgaa ggcggggtag atgttcgggg
      301 caacctccat gcgatagcca aaaagggcga tttgtggcac gccggggagg gcgaagaaat
      361 cgagctggcc gccggtggcc tgaaccaacg tctcgcccaa cagcgtgaaa tgcccaaagc
      421 cgtaattcca gtaaccgatt ccttccgaac agtatccgtc cggtgtaaaa ccctgaagga
      481 aatactggat atatttttca gctgcggcca cgaagaaacc gcgacgctct ggagacggaa
      541 tattctccag ggcggctccc accacaccgg ccagacacac cgcattccaa ttgtttgttc
      601 cggtaatcca ccacaaccgc ggattgcccg tattcacggc ctgttcgaac ggtgtaaaga
      661 tacgccggtc cagttcactg gcgatgagac ggcggcattc gggcgacagc ttttctgcca
      721 accaatagtc gatggtcgcc atattccagg cggtgcttgc agcccctagg tcgatttcca
      781 cggtccggcc atagaaattg tcgagccgtc catcgtgggc gggcattacc caggtctttt
      841 cctggcagat ggcggcaatg gtcttttcca gctcggccag gaatcgcccc cggttctcga
      901 gacattcggc tagggccaaa ttcccaagcc gccctcgccg tgcggacatg cgggactcat
      961 accggcgccg attgccggtt cgggaaaagt ccagatatag ctcgtccggg acctcagcaa
     1021 tgggccggga aagttcctcc tcggcctcgg caactacctg acgaaacgct ttatccttgg
     1081 ccaggacatc ccaaatctct cgctgcgaaa ttggggcgcc aacccccaca ggcttcggcg
     1141 ggagatgctt ggcccaatag gccgccctct cggcagaaac acctccagca atcggctcgg
     1201 cggtggctcg atcgcccata cccattccca gtaagacaac cgccatgatc gccactaagt
     1261 tgccagcatt ttgacgacaa aggataaaag tttttcgtga cataatgacc tccccagaca
     1321 aaggttggaa cacgatgcgg gcaaatctca attgggccaa aacggtcagc gtcgcctctc
     1381 aggcgagacc gtccatggtc attccaaaat gcttgagcac aacgtgaaag atgtcggtgt
     1441 ctgtcacggg ttcccgggga aacacacccg gacgcgagct aatcgccacc gttcgctgga
     1501 agcgctcacg caccggggca ccatgcgacc catgcaccaa ctccggcttt aggggaatac
     1561 cgcgtgtggc tggatcaaaa aacaactcca ctggatcata acctggcttg cggtgaatat
     1621 ctacctttcg cgcaaatggc ggtgccttgt ggtcctcgaa ccaataataa tatgcgaacc
     1681 acgtatcggg ctgggccgtc aggatgacct caccacttcg cgggtgatcc atatgatact
     1741 tgctccgctg ctcacccgcc agcacttcat caattcccgg cagaccttga aacaaccgca
     1801 tcacgcggtc aatgatctcc ggttcgccat cacggacaaa aacatgggcg atctgatggt
     1861 cggccagggc ccaggccgca ctggtggtgt ccaggtactc gccgtctcgc tcctcgcgga
     1921 cagaaagtaa tcccgcttcg cgcaagatcc gattgggata aatcactcgt tcgacgggcg
     1981 tgatggcata ttctccggca atcagccaac agggatcacc atcataagca tctcgcaaac
     2041 cgttcatcaa ttgtccgagg acctcgtcaa gctcaagtac ggccttgccg gcggcaacgc
     2101 tgtccgggcc atccttctgg gcagcgtaat ctaaatgggg caagtaaata taaaacaagt
     2161 tcggtcgtgc cgctttggcc aattcaatgg cagactcgac gatccaccga gtactctcga
     2221 tattggccaa gggaccccaa taacgatgca gcggaaaatc ccccagtttc tgccgcaact
     2281 cgccatagag gttctccggg cgcgtgtagc accacatggt ttcactgccg tcggggttgt
     2341 gaatcggcgc tggcgtacaa atgaagtcgg ccccagcctc tttggcatgt tgcggaaacc
     2401 aaatggcgct ggtcaaccct cgctcatgat gataaagcaa gtcccagagt tgtggcactt
     2461 caatgcaatc gttgggcgag gtccacattt cgacctcgtc gcgatcacgc cagtagattc
     2521 cgttcgcaat gatgccatgc ctcttgggcg gtcgcccggt cgtcatgttc gcctggacgg
     2581 ggcaggtcaa agcgggaaaa ctcggaataa ggtccacaat ctcgccgcct gccatcattt
     2641 ccttgaggtt ttccatccgg gcgacatcct tttcgcgaag gttcgccacc gatat
//
LOCUS       DAOPTR010000526         2693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_30165_length_2693_cov_3.889310, whole genome shotgun sequence.
ACCESSION   DAOPTR010000526 DAOPTR010000000
VERSION     DAOPTR010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30165_length_2693_cov_3.889310"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..2573)
                     /locus_tag="PKI05_10240"
     CDS             complement(273..2573)
                     /locus_tag="PKI05_10240"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOA52624.1"
                     /translation="MTSTKSQSSTIRLNVLGMHCAGCAARVERALRAVPGVDAAQVNL
                     VTQKAVVRLADPNTSPERLIQAVVDAGYKATVVEEAVATRATATSPWEIQDQEQRLRA
                     RQVVVGSVGLVVVLAVMLFPGLGEWVTPWPAIIAATVVQVYLGWGYLASAWRQGRRGG
                     VSMDTLIAIGTWTAYVAAIAETTGGFHTVHQSSHGSGLSMYFSDAVMILTFITLGKYL
                     EIRSRFRASLAIRRLMDLSPQVALVIRGQTVAEVPIDKIQRGEILRVRPGEKIPLDGS
                     VTQGQSDVDESWLTGESLPVVKQPGDRVFAGTVNLSGTILVRVTKTADETLLSQVIRL
                     VEQAQEAKPQLARLADRVVAWFVPIVLIVAGVTFVVWGPVLSQWQLAVNGLVAVLVVA
                     CPCALGLATPTAVLVASGRAASRGILVKDARAYEAAARVNVVVLDKTGTVTLGQPQLV
                     AIEAAPGVDEDRLLAAAGSAEQTSLHPLAGAVLAEVRRRKLNLADVDSVEILPGVGLR
                     VQSGGHTIVVRTAGVSNEEVTQPAELSSRPLNEEKIAPMPRADRGRKDERTMIRVVVV
                     EDGQLLGTLGFADQVSETSREAINALRKRGFRVVLLTGDRAENAAQVAAEVGITEVFS
                     EMTPSEKHTLIRQLQAEGNVVAMVGDGINDAAALAEADVGMAMSAGADIAKESASIVL
                     VRRDLRAAVDMLDLGRATVRVIKRNLFWAFAYNVALIPLATGLFFPWTGVLIPPPAAA
                     ACMAASSVSVVLSSLMLGKDVRPARL"
BASE COUNT          598 a          851 c          704 g          540 t
ORIGIN      
        1 atttgtcgta gttgacaagt cgccccggtt cctctgccag ccattgtcgg acaaagttac
       61 tgccgatgaa acctgcacca cccgtcacaa ggattgtgtc catggttcca ctcgcttcga
      121 gagtagcctg atgtgccgcc tctcatgaac aaggcgaaga acgcgccctc agctcccgta
      181 tgatctattt taacctcgct ttggtgtctg tcactctgaa cagcagccgt tggggagatc
      241 acggcattct cttcaaaagg ggggattcca ccttataaac gggctggccg cacgtctttt
      301 cccaacatca aactgcttaa caccacggag accgaactgg cagccatgca agccgctgct
      361 gctggtggtg ggatcaagac acccgtccac gggaagaata ggccggttgc caaaggaatg
      421 agtgccacgt tgtaggcaaa ggcccagaaa agattgcgtt tgatcacgcg gacggtagcc
      481 cgacccaggt caagcatatc caccgcggcc cgcaggtcgc gccgaacaag gacaatgctg
      541 gccgactctt tggcgatgtc agctccggca ctcatcgcca tgcctacatc ggcttcggcc
      601 agggctgcgg catcattgat gccgtctccc accatcgcca ccacgtttcc ttccgcttga
      661 agttgcctga tcaaagtgtg tttttcggaa ggtgtcatct ccgaaaacac ctccgtgatg
      721 ccgacttcgg ccgctacttg ggcagcgttt tctgcgcgat cccctgtcaa taaaacgact
      781 cggaatcccc tttttcgcaa ggcgttaatg gcctcgcggc tagtctcgct gacttgatcg
      841 gcaaatccca gtgtccccag caactgccca tcttccacca cgaccacgcg gatcatcgtc
      901 ctttcatctt tacgcccacg gtccgccctc ggcatcggcg caatcttttc ctcgttcagg
      961 ggtctcgacg aaagctcggc aggctgggta acctcttcgt tggagacccc ggctgttcgc
     1021 acgacgatgg tgtgtccacc ggattgcaca cgcagtccca cgccgggcag aatttccacc
     1081 gaatccacgt cggcaagatt tagctttcgg cggcggacct ccgccaagac cgctcccgcc
     1141 aaggggtgaa ggctggtttg ttcggcacta ccggcggccg caagtaaacg atcttcgtcc
     1201 acgcccggtg ccgcttcgat cgcaacaagt tgtggctggc cgagcgtaac tgtgcccgtc
     1261 ttatctagta cgacgacatt cacgcgggct gctgcttcat aagcccgcgc atctttcacc
     1321 aagattcccc gggaagctgc ccggccactt gctacgagca ccgccgtggg cgtcgccaga
     1381 ccgagggcac acggacaagc caccacgagt acagcaacca gaccattcac ggcaagttgc
     1441 cactgactca agacaggtcc ccaaacgaca aaagtcaccc ccgcaacaat tagaacgatt
     1501 ggcacaaacc atgccacaac acgatcggcc agtcgggcca actgtggctt cgcctcctgg
     1561 gcctgctcga caagccgaat gacctgcgaa agtaaggtct catccgctgt cttcgtcacc
     1621 cgcacaagaa tcgtccccga gagattgacc gttcccgcaa agaccctgtc accaggctgc
     1681 ttgaccacag gcagggattc tccggtgagc cacgattcat caacgtcgct ttggccctgt
     1741 gttacggagc catccaaagg gatcttctcg ccagggcgaa cccgcaagat ctctccccgt
     1801 tggatcttat caatggggac ctcggccaca gtttgtcctc gaatcaccag cgcaacttga
     1861 ggggataagt ccatcaggcg gcggatcgcc aatgaagcgc ggaaacggga acgaatttcc
     1921 aggtatttcc ccaacgtgat gaatgtcaga atcatcacgg catcgctgaa atacatcgac
     1981 aagcctgatc catggctcga ctgatgaact gtgtgaaacc caccggtggt ctcggcaatc
     2041 gccgcgacgt aagccgtcca agtgccgatg gcgatgagcg tatccatact gacgccgcca
     2101 cggcgtccct gtcgccaggc cgaggcaaga tatccccatc ccaaatagac ctgcaccacc
     2161 gtggccgcga taatggccgg ccagggagtc acccattcac ccagtccagg gaagagcatc
     2221 actgccagaa ccacgaccaa gcccacggag ccaaccacga cctgacgtgc gcggagccgt
     2281 tgttcttgat cttggatctc ccatggactt gttgccgtgg cacgggtggc gaccgcctct
     2341 tcaacgacag tcgctttgta tcctgcatcc acgactgctt gaataagtcg ctccggcgag
     2401 gtgttgggat cagcgagccg aacgacggcc ttctgggtga cgagattgac ctgggcggca
     2461 tccacgccgg gaaccgcacg gagtgccctc tccacccgcg cggcacatcc cgcacaatgc
     2521 atccccaaaa cattcaggcg aattgttgac gattgcgact tggtcgacgt catggtgcaa
     2581 agtttcccgg tccaaaaaag ggcgggcaag cggcccccta tgggaaataa tagtaaaacg
     2641 tgattcaaag acgagggttt gggacttgtt caaatgtgat ctatgcccta aaa
//
LOCUS       DAOPTR010000527         2691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_30191_length_2691_cov_3.610395, whole genome shotgun sequence.
ACCESSION   DAOPTR010000527 DAOPTR010000000
VERSION     DAOPTR010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30191_length_2691_cov_3.610395"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..>2691
                     /locus_tag="PKI05_10245"
     CDS             41..>2691
                     /locus_tag="PKI05_10245"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOA52625.1"
                     /translation="MRRSIVVVCCSVFLALHSVGLMSGEELSPARVTYLRCEYRVNPL
                     GIGTTRPRLSWIIEDPRTGAAQTAYQIIVASSSEILAENRGDLWDSGKVISSEMNQIE
                     YAGKPLQSRMKCYWKVRIWDANQQATPFSEPAMWSMGLLDPQEMKARWIQASQPLVHP
                     AKGTQLDFGDCPWVWYPEENTDPREKAPAGPRYFRKVIELPSEKAIKRARLLLTADDS
                     FELFCNGQPAGRGAGYNAPQIADLTNRLQPGRNTLAIVATNASPSPAGVCGRLRVEFE
                     SGESSEWLIDSTWKAFPRAVAGWNQVDFDDSAWPFAVVVGKMGDKPWGAISAQGLDIF
                     GCGLFRKAFRIDKPIQSATLYMSALGVGEIHINGQLVNDEYFAPGWTDYRKRVYYNTY
                     DVTNLVRQGDNAIGAILAAGWYAGAIGWKSERFHFGRDPKLWVQLEIVTSDGERMTIV
                     SDRTWKYTFGPHVEAEFLAGETYDARLEIPNWSLPGLDESQWQPVQEANDVQVLLEAQ
                     RGVPVIRTGELPAKSVSEPKPGVFVFDLGQNFAGFARLKVRGPKGTAVQLRFAEMLNP
                     DGTIYTTNLRSARATDTYILKGEGEEVWEPRFTFHGFRYVEVTGYPGRPPLDAITGIA
                     INSDIPLVGSFRCSSEMVNQLYSNIVWTQRANFISVPTDCPQRDERLGWTGDAQVFIR
                     AATYNADVAAFFNKWLVDLEDAQGPEGDFPDVAPRVVALGGGTAAWADAGVICPWTIY
                     LVYHDRRVLEEHFDAMERWIAYCQKHSQNLLRPAAGYGDWLSIKADTPKDVLATAFFA
                     YSTHLTAECARALGLAEKAAYYDKLFEEIRAAFQKAYVSPDGRVKGDTQTCYVLALYF
                     NLLPEALRPKAAQYLVEDIRKRDTHLST"
BASE COUNT          593 a          703 c          772 g          623 t
ORIGIN      
        1 taaagaggtt tctttcatgt tgtcatagga ggatcgcgga atgcggcggt caattgttgt
       61 ggtctgttgc agtgttttct tggctcttca ctcggtgggt ctgatgtcag gggaggagct
      121 aagtccggca cgtgtgacct atcttagatg cgaatatcgt gtgaatcccc tcgggatagg
      181 gacaactcgc cctcgcctca gttggatcat cgaggaccct agaactgggg cagctcagac
      241 agcttatcaa atcattgtgg catcaagctc cgagattctc gccgagaatc gcggcgacct
      301 atgggattcg ggcaaagtta tttcctcgga gatgaaccag attgaatacg caggcaaacc
      361 cttgcaatct cggatgaagt gctactggaa ggtccgcatt tgggacgcca atcagcaggc
      421 gacgccgttc agtgaacctg cgatgtggtc gatggggctt ctcgacccgc aggagatgaa
      481 ggcccgttgg attcaagcct cgcaaccact tgtccaccct gccaaaggga cgcagttaga
      541 ttttggagat tgcccctggg tttggtaccc cgaggagaac accgaccccc gagaaaaagc
      601 ccctgcggga ccccgctact tccgaaaggt aatcgaatta ccctcagaga aggctatcaa
      661 acgggcacga ctgctactga cggccgacga ttcgtttgaa ttattctgca acggtcaacc
      721 ggcgggacgt ggtgcgggtt acaacgctcc gcaaatcgca gatctgacca atcgtttgca
      781 gcccggtcga aacaccttgg cgattgtcgc cacaaacgcc tcgccgtcac cggccggcgt
      841 gtgtggtcgg cttcgtgttg agttcgaatc tggtgaatcc agcgaatggc tcatcgactc
      901 aacgtggaaa gcctttcccc gggctgtggc tggctggaat caggtcgatt tcgatgattc
      961 ggcctggcca tttgccgtcg ttgtaggcaa gatgggcgac aaaccgtggg gagcaatttc
     1021 ggctcaaggg ctcgacattt tcggctgtgg attgttccgc aaggcatttc gaatcgataa
     1081 gcccatccaa agcgctacac tctatatgag tgccttggga gttggggaaa tccatatcaa
     1141 tggtcagctc gttaacgatg aatacttcgc tcccggttgg accgattatc gcaagcgagt
     1201 gtattacaat acttacgatg tgacgaatct tgtccgacag ggcgataacg caatcggcgc
     1261 catcctcgcg gccggctggt atgctggggc gatcggctgg aaaagtgaac gatttcactt
     1321 tggccgtgat ccgaaactat gggtccaact ggaaattgtc accagcgatg gcgaacgcat
     1381 gaccattgtc agcgatcgca cctggaaata caccttcggt cctcacgttg aagctgaatt
     1441 cctggcaggc gaaacctacg acgcccggct ggagattcct aactggtcac tgcccggact
     1501 cgacgagagc caatggcaac cggtacagga ggccaacgac gtccaggttc tcctcgaagc
     1561 gcagcgcggc gttccagtta taaggaccgg tgagttgcct gccaaaagtg tctccgaacc
     1621 taagccaggg gtgttcgtct ttgatctcgg tcagaatttc gcgggttttg cgcggttaaa
     1681 agtccgcggt cccaagggaa cggcagttca gctacgattt gcagagatgc tcaaccctga
     1741 tggaacgatt tacacgacaa acctccgctc ggctcgcgct accgacacct acatcctcaa
     1801 aggagagggc gaagaagttt gggagccacg gtttacattc cacggtttcc gttatgttga
     1861 agtcactggg tatcctgggc ggccaccttt ggatgcgatc acggggattg ccattaactc
     1921 ggatatccct ttggtcggga gctttcgatg ctcaagcgaa atggtcaacc agctctacag
     1981 taatattgtg tggacgcaac gggcaaactt catctctgtc ccaacagatt gcccgcagcg
     2041 agatgaaaga ctgggatgga ccggcgacgc ccaggtcttc atccgtgctg ccacttacaa
     2101 cgctgatgtg gcagccttct ttaacaagtg gttagttgat ttggaagacg cccagggacc
     2161 tgaaggcgat tttcccgatg tggcgccgcg tgtcgtggcc cttgggggtg gtacagccgc
     2221 ctgggcagac gccggggtca tttgtccttg gacgatttac ttggtttacc atgatcggcg
     2281 cgtgcttgag gaacactttg atgcgatgga gcgatggatt gcgtactgtc agaaacacag
     2341 ccagaatcta cttcgtcccg cggccggtta tggtgattgg ctttcgatca aggccgatac
     2401 gcccaaggat gtgctagcaa ccgcattttt tgcctacagt acgcacttga cggcggagtg
     2461 cgccagggca ctcggtctgg ctgaaaaagc cgcgtattac gacaaattgt ttgaagagat
     2521 tcgtgccgct tttcagaagg cctacgttag cccggatggc cgcgtgaagg gtgatacgca
     2581 gacttgctac gttctggccc tctatttcaa tctgttgccg gaggcgttgc gcccgaaggc
     2641 ggctcagtac ctcgttgaag atatccgaaa gcgcgacacg catctttcca c
//
LOCUS       DAOPTR010000528         2689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_30226_length_2689_cov_3.067198, whole genome shotgun sequence.
ACCESSION   DAOPTR010000528 DAOPTR010000000
VERSION     DAOPTR010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30226_length_2689_cov_3.067198"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..1269
                     /gene="hemC"
                     /locus_tag="PKI05_10250"
     CDS             301..1269
                     /gene="hemC"
                     /locus_tag="PKI05_10250"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOA52626.1"
                     /translation="MTSGKTLRLGTRGSLLARWQAEWVASQLRAHGVEVEIVPVLTSG
                     DRNQHWAVESLGGAGVFTKEIQLALLANEVDLAVHSMKDLPTQPVPGLAIAAIPKRGP
                     VGDVLISRTAQTFEQLPSEAIIGTGSRRRAAQILHYRKDLTISPLRGNLDTRLRKLDS
                     GLYDAIVLAQAGLERLNITGRSCEPLTPRFLLPAVGQGALAVECRLDDEETRQCLTVL
                     NDPISAAEVTAERSFLRHLEAGCLAPVAAWCRAVGSSLLLTGRVLSVDGTTKLEVSCL
                     GDIENPEELGRRVAQLLIAQGAKDLIRQAKTIPMAPPPDAKPDIDD"
     gene            1518..2279
                     /locus_tag="PKI05_10255"
     CDS             1518..2279
                     /locus_tag="PKI05_10255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HOA52627.1"
                     /translation="MPYGIYLSAEGAHAQDRRLTILANNLANVETVGFKRQLAICQAR
                     YTEPVNRGLESPGTGRWSDLSGGVVVSMTKTDFSPGPLKETRSPTDFAIRGEGFFTVQ
                     KGDEVFLTRAGNFQLNGKGQLVTQYGGEQYLVLNDAGQPIVIDGSRPWRVNPQGVVEQ
                     EGLLQNIAVVRPASKDSLVPVGENLFRPTAQPSPVPLAQRQVAQGYLEMSSVQPTLAM
                     VELLEASRLLEANLAMVQTQDQMLSNLFNRVLRAS"
     gene            2346..>2689
                     /locus_tag="PKI05_10260"
     CDS             2346..>2689
                     /locus_tag="PKI05_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HOA52628.1"
                     /translation="MSVQTLYSAATGMLAMEKKLDVIANNLANIETTAFKKGRANFED
                     LFYSHEVFPGAQDTSGEMTPVGISIGLGSRLSGIQTDFRQGAFQQTSNQLDVAIEGAG
                     FFQVMDPSGQIY"
BASE COUNT          590 a          733 c          764 g          602 t
ORIGIN      
        1 attccgagta ggacgttcca catgaggtgc gagcatccgt ggccgtgact cggtggggtg
       61 ctccggcgcg tcgtgtttca gcacaacgat cagtggatcg ccctcgcacg agaataggcc
      121 tccacgggtt tcgcatcggg caaccaccgc gtgatcccac aaatgctttt gggcaatcta
      181 gttgaacagg cgttggtgcc atacccccgg ggacttttct ttggtttata atcggatgct
      241 ttcccttcga ttgaaggttt ggtggcatcc gtgtgtctct tggacttgtg atttggaacc
      301 atgacgtccg gtaaaacgct ccgcttggga actcggggga gcctcttggc tcgatggcaa
      361 gccgaatggg tagcctcaca attgcgtgcg cacggtgttg aagtcgaaat cgttcctgtt
      421 ttgacaagcg gtgatcgaaa tcaacactgg gccgttgagt cgctaggtgg tgccggggtt
      481 ttcaccaagg aaatccagtt ggcccttctt gcgaatgagg tagatttggc tgtccacagc
      541 atgaaggact tgcccacgca gcccgttccc ggattggcga ttgccgccat tcccaagcgg
      601 ggcccagtgg gagatgttct catctcccga acggctcaaa cgttcgaaca acttccgtcg
      661 gaagcgatca taggaacggg cagtcgccgc cgggcggccc aaatcttgca ctatcgaaaa
      721 gacctgacca tttcacccct ccgcggaaac ctcgatactc gcttgcggaa attggattcg
      781 ggcttatacg acgccatcgt tttggcccag gccggtctgg agcgactcaa cattaccggc
      841 cgaagctgtg aacccctcac gccacgtttc cttctccctg ccgtgggtca gggtgcactg
      901 gcggtggagt gtcgtctcga tgacgaggag acacgccaat gtctcaccgt cctgaatgac
      961 cccatctccg ctgccgaagt caccgcagaa cggagcttct tgcggcatct tgaagcgggc
     1021 tgcctcgcac cggtggcggc ctggtgccgt gctgttggtt cgagtctgct gctgacggga
     1081 agggtcttga gcgttgatgg gacaacgaaa ctggaggttt cttgccttgg agacatagag
     1141 aatccagagg aattaggaag acgtgtggcg caactgctga ttgcgcaagg ggcaaaagac
     1201 ctgatccgtc aagccaaaac aatcccgatg gccccaccgc cagacgccaa gccggatatt
     1261 gatgactgaa tcagtcccag gggctttcgg cagtttctgc agattccccg gctgattctg
     1321 cgtctcgacg aatgatctct agatcgccag cttcttgacg agatcgccgg gttcttcgta
     1381 agttcttttc tgaaaaaggt ttatgtcacc atattcatcg tgcatcgtgc ctggcacggt
     1441 gtttgcaatg atgaggcggc cgcggaaaaa ggattttctc gaaacgtcac gcgactgaac
     1501 ggattttgac gagtaccatg ccatacggga tctatctttc ggcagaaggt gcccacgctc
     1561 aggaccgtcg cttgacaatc ttggccaaca acttggccaa tgtcgagacc gttggattta
     1621 agcgtcaatt ggccatttgt caggcccgtt acacagaacc cgtcaatcgc gggcttgagt
     1681 caccggggac cggtcgatgg tcggacctca gcgggggcgt ggtcgtttcc atgaccaaaa
     1741 ccgatttttc cccaggacct ttgaaagaga cccgcagccc caccgacttt gccattcgcg
     1801 gcgagggttt tttcacggtg cagaaaggag acgaggtctt tctcactcgg gcgggcaatt
     1861 ttcaactcaa cggcaaagga caacttgtca cccagtacgg tggcgaacag tacttggtcc
     1921 tcaacgacgc cggacaaccg attgtgattg atggatcacg gccttggcgg gtcaaccctc
     1981 aaggagtggt ggaacaggag ggactactac agaacattgc cgtggtgcgg ccagcatcaa
     2041 aggattcact tgtgccggtg ggtgagaatc tctttcggcc caccgcccag cccagccccg
     2101 tcccgctggc ccagcgtcag gtagcacaag gatacctgga gatgtcctct gtgcagccga
     2161 cgctcgccat ggtggaactc ttggaggcct cgcggctact agaagccaat ctggcaatgg
     2221 tccaaacaca agatcaaatg ctgtccaact tgttcaatcg ggtgcttcgt gcctcctaaa
     2281 gacagcgcaa ttcaagacga cttgctaaca cacagttgac ctgagcagta gaaacgaggg
     2341 aagaaatgag tgtccaaaca ctttattcgg cagcaacagg catgttggcg atggaaaaga
     2401 aactggacgt catcgccaac aacctggcca acattgaaac gacggcgttc aagaaaggcc
     2461 gggccaattt tgaagatttg ttttatagcc atgaagtctt ccccggtgcc caggatacct
     2521 cgggcgaaat gaccccagtc ggcatttcga ttggcctggg ctctcggttg tcgggcattc
     2581 aaacggactt ccgccagggg gctttccagc aaacctcgaa ccagcttgat gtggcgatcg
     2641 aaggggccgg tttcttccag gttatggatc cgtcagggca aatctacta
//
LOCUS       DAOPTR010000529         2688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_30238_length_2688_cov_3.612229, whole genome shotgun sequence.
ACCESSION   DAOPTR010000529 DAOPTR010000000
VERSION     DAOPTR010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30238_length_2688_cov_3.612229"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="PKI05_10265"
     CDS             <1..859
                     /locus_tag="PKI05_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52629.1"
                     /translation="SKTIAVFSPKGGVGKTTVAENMAAIFGMWAKNQTEKTGIPIRVA
                     LLDFNLDGSSGPYTWCPDENPKTASLWRDLDGSTVRWQDVSGAMTYHEGANVWYLAPP
                     FRPDEKLEFSLPLVEAILTGCKRYFHITIIDNGVALLGRDVVLSVFSASSDVLLVSDF
                     SYKTMRLMVDAYNNELKRLLGDTSKASLVLNRVSPKWFTTRDFVYALSKGIGTALPLK
                     GELPEDPNFRTEGGEFKGAPLVYIYPDSPFSNAVSGLCRAVSGVDIVWQTKKEKRRFR
                     LFPWVKKGK"
     gene            865..1296
                     /locus_tag="PKI05_10270"
     CDS             865..1296
                     /locus_tag="PKI05_10270"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HOA52630.1"
                     /translation="MAAQDLALLIGCLLPMSICIYTDFKNYILPNWLTAFIALGGLAT
                     ALLFNRMPDALLGAVLAGGAFVITSFFGDIGGGDIKLAFGIGMWFGWHGGASVILLGC
                     LLATVWSIPQMMKSGIRNVRVPLGAFLGIAAWAYVLAQYLT"
     gene            1311..2636
                     /locus_tag="PKI05_10275"
     CDS             1311..2636
                     /locus_tag="PKI05_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOA52631.1"
                     /translation="MSSMYAIKMGNPIQQTEADLEKVREMFFARAWSKNPDALTNRDE
                     ARRLLKDIIVEIGGSLSLVDALLRDIYGFGPLSELMDDPDVSDIWVDDYKSIWYEKDG
                     KLFEWHKTFMSEEHLKKFAVRLAASTGRKVDEARPIEDFRLLDGSRAVVFLSGVSVRG
                     TSLVIRKFTRLFTLEELAERKLFPVWMIRLFHYMVKARSNIFVAGAFGSGKNTLMNAL
                     LLRVNRSERLVFIEDPAETKVGLPDPDRPDLPIPRSVVFEPRKAGVEGEGEVSMDLIF
                     EKVLRSKPSRVICSECRDGTTAYWVLQAMNLGISGSLSSLHAEAPEEVPLRLADMLAT
                     YRTGAYSSFSSRAGAIAAAELIIYLGQINGHRRVLDICEIRRENKPDAFPEVVPLYSF
                     EYKGDGEDGEPLGELKPTGEIPKFLDKRKVAMSLSPEELEEMRRFFNAS"
BASE COUNT          630 a          594 c          799 g          665 t
ORIGIN      
        1 gtctaagacc atagcggtct tctcgccgaa aggaggggtc ggaaaaacga ccgtcgctga
       61 gaatatggca gcgatattcg ggatgtgggc caaaaatcag acagaaaaaa cagggattcc
      121 gatacgtgta gccctcttag actttaacct ggacgggtcg tccgggccgt acacttggtg
      181 ccccgacgag aatcctaaga cggcgagctt gtggcgggac ctcgacggct cgacggtgag
      241 gtggcaggac gtatccggcg cgatgaccta ccacgagggc gcgaacgtgt ggtatctcgc
      301 tccgcctttc cgtcccgacg agaaattgga attcagcttg ccgctggttg aggccatttt
      361 gacgggatgc aagcggtatt tccatataac catcatcgac aacggcgtcg cattgttggg
      421 acgtgacgta gtgttgtcgg tgttttcagc ttcctcagac gtgctgttgg tctcagattt
      481 cagctacaag acgatgcggt taatggtcga tgcctacaac aacgagttga agcgtttgtt
      541 gggagatacc agcaaagcgt cgctggtttt aaaccgggtg tcccccaagt ggttcacgac
      601 ccgagatttc gtttatgcgc tgtcgaaggg gatagggacc gcattgccgc ttaaaggaga
      661 gctgccggag gaccccaatt tcaggaccga agggggcgag ttcaagggcg cgccgctggt
      721 ctatatatat cccgattccc ctttctctaa tgctgtgtca gggttatgcc gcgccgtttc
      781 cggcgttgac atcgtatggc aaaccaaaaa ggaaaagaga cgatttagac tctttccttg
      841 ggtcaagaaa ggtaagtagc agtcatggca gctcaggact tagcgttgct tattgggtgt
      901 cttttgccga tgtcgatctg tatttacacg gattttaaaa actatatatt gccgaactgg
      961 ttaacggcct ttattgcttt aggtggcttg gcaacggcat tgttgtttaa ccggatgccg
     1021 gacgcattgt taggagccgt cctagcagga ggagcattcg tgattacctc attttttgga
     1081 gacatcggcg gaggcgacat caagctggcc tttggcatcg gcatgtggtt tgggtggcac
     1141 ggcggcgcaa gtgtgatctt attgggatgc ttgttggcaa cggtatggag tataccacaa
     1201 atgatgaaaa gcgggattag gaatgttcgt gtgccgcttg gagcatttct ggggattgcg
     1261 gcttgggcat atgtcttagc tcagtattta acttgaaaga agggattcag atgagcagca
     1321 tgtatgcgat aaagatgggt aatcccatac agcaaacaga ggcggatttg gaaaaagtac
     1381 gggaaatgtt cttcgcccgt gcatggagca aaaacccgga cgcattaacc aatcgcgacg
     1441 aagcgcgccg gttgctgaaa gacatcatcg ttgaaattgg agggtcgttg agtttagtgg
     1501 atgccttgct cagagacatt tacggcttcg gcccactgtc cgagcttatg gacgaccctg
     1561 atgtaagcga catatgggtc gatgattaca aatccatatg gtacgaaaag gacgggaagt
     1621 tgttcgagtg gcataaaact ttcatgtctg aagaacatct gaaaaaattt gccgtccgct
     1681 tggccgcctc gaccggcagg aaagtggacg aagcgcgccc catcgaggat tttaggttgt
     1741 tggacggctc cagagccgtg gtattcttgt cgggcgtgtc ggtgcgcggc acaagccttg
     1801 tcatcaggaa gttcacccgg ctgtttacgc ttgaagaatt ggccgaaaga aaactatttc
     1861 ccgtgtggat gatacgtttg ttccattata tggtcaaggc gcgaagtaat atctttgtgg
     1921 ccggggcttt tggttcagga aaaaatacgt taatgaatgc ccttttgttg cgggtcaacc
     1981 ggtcagaacg gcttgtattc atcgaggacc cggcggagac caaggtcggc ttgcccgacc
     2041 ctgatcggcc cgacctcccc ataccgcgat cagtagtctt cgagccccgg aaagcagggg
     2101 tagaaggaga aggtgaggta tcaatggact tgatcttcga gaaggttctt cgctcaaaac
     2161 ctagccgggt gatttgttcg gaatgccggg acggaactac cgcctattgg gtattgcaag
     2221 cgatgaacct tggaatatcg gggtcgttgt cgtccctcca tgcggaagcg ccggaggaag
     2281 ttccgttgcg actagctgat atgttggcga cctataggac gggggcgtat agttcttttt
     2341 cttcccgtgc cggggcaatc gcggcggcgg aactgataat ttatctaggg cagataaatg
     2401 ggcaccgacg ggtgttagac atttgcgaga tccgccggga gaacaagccc gatgcgttcc
     2461 cggaggtcgt gcctttgtat tcttttgagt acaaagggga cggtgaggac ggagaaccgt
     2521 taggagaatt gaaaccaacc ggagagatcc caaagttcct ggataaacga aaagtcgcca
     2581 tgtcgttgtc tcctgaagaa ctcgaagaaa tgaggcggtt tttcaatgca agttagcgtc
     2641 aatctcattg cggtgtggat aactgtggtc ctgtctttcc tgtgtttc
//
LOCUS       DAOPTR010000530         2687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_30254_length_2687_cov_3.082067, whole genome shotgun sequence.
ACCESSION   DAOPTR010000530 DAOPTR010000000
VERSION     DAOPTR010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30254_length_2687_cov_3.082067"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PKI05_10280"
     CDS             <1..632
                     /locus_tag="PKI05_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOA52632.1"
                     /translation="PRVGIDTVQAEDMRYARQLGYHIRLLAVAELIDGELELHVSPTL
                     VREGTPLGEVRGAYNAVRVVGDAVGRVFFHGLGAGQMPTASAVVADIIDTAVGRAAIT
                     FRTLRLWSNDGPHIGLRNPDMARSRFYLRFHVQDRPGVLAEIACILGRNQISIASVIQ
                     HQTEEETEGVVPLVIMTHTAAEGPMRKAMAEIDRLPVVRPGSVWMRVRD"
     gene            709..1926
                     /locus_tag="PKI05_10285"
     CDS             709..1926
                     /locus_tag="PKI05_10285"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOA52633.1"
                     /translation="MRKYAIVIPDGCADEPQESLGGKTPLEAADIPAMDAVARAGVVG
                     QANHVPRSLPPGSDVANLSLLGYDPVAHYTGRAPLEAAAQGIPLGPNDWAIRCNLVTI
                     IDQVMRDFTADHISTGEARELMTTLQEKLGTPQLQFFPGVSYRNLLIYRGGDKPAPFS
                     RDTVTVPPHDLTDQSVIDAYPRGPGSDLLTRLMCESAEIFANHPVNVARQKAGKLPAT
                     NIWLWGLGGAPQLPPFQQRYGVTGAMITAVDLLRGLAALFGWRRIDVPGATGYIDTDY
                     AAKGRYAIEALKEVDLVCVHIEATDEASHEGDAEAKIQALEAIDQKIVAPLWEALQNY
                     GDYRILVSPDHPTPLRLKTHAHGYVPFAIAGTGIPTSGATGYNERTAEESGIIFEEGW
                     RVMEYFLRGESPP"
     gene            2048..>2687
                     /locus_tag="PKI05_10290"
     CDS             2048..>2687
                     /locus_tag="PKI05_10290"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOA52634.1"
                     /translation="MAVLVQKFGGTSVADAEKILAAARKAIRAKLQGYQVVTVVSAMG
                     HTSDHLIELAKQIDPEPPAREMDMLLSTGEQVSVALMAMAIHRLGHRAISMTGAQMGI
                     YTDSTHTKARIRSISTDRIRQLLDEGNIVIAAGFQGITDTADITTLGRGGSDTTAVAL
                     AAALRAETCEIYTDVDGVYTTDPRLVPEARRVSRISYDEMLELATAGAGVMHN"
BASE COUNT          579 a          726 c          809 g          573 t
ORIGIN      
        1 tcccgcgggt ggggatcgac accgtccagg cggaggatat gcggtatgcc cgacagttgg
       61 gatatcacat tcgcctgttg gccgtagctg aactaatcga cggtgagctt gagttgcacg
      121 tatcgcctac attggtccgg gaggggacac cccttggtga ggtccgcggg gcatacaatg
      181 ccgtccgcgt cgtgggtgat gccgttggtc gggtcttttt ccatggcttg ggagctggcc
      241 aaatgccgac cgcctcggcg gtggtagccg atataatcga cactgcggtt ggtcgggcgg
      301 ccatcacatt ccgcacgctg cgattgtgga gcaacgacgg tccccacatt ggcttgcgta
      361 acccggatat ggcccgaagt cggttttacc ttcgcttcca tgttcaagat cggccgggtg
      421 tcctggccga gatcgcctgc atcctgggcc gaaaccagat ttccatcgca tctgtcatcc
      481 aacatcagac agaagaagag acggaaggcg ttgtgccatt ggtgatcatg actcataccg
      541 cggcggaagg ccccatgcgg aaggcgatgg ctgagattga cagattgccg gtggttcgtc
      601 cgggcagtgt gtggatgcgg gtgcgggatt gataggagtt ttgtggctgt gaacctttaa
      661 ttcctgagct tcctcgtagt aaatctacca gacaatggtt caagcgatat gagaaaatac
      721 gcaattgtta ttcccgatgg ctgcgccgac gaaccccagg aaagcctcgg gggaaagact
      781 ccccttgagg ccgctgatat tccggccatg gatgctgttg cccgcgctgg ggttgtcggc
      841 caggccaatc acgttccacg ctccctgccg ccgggctcgg atgtggccaa tctgagtctt
      901 ctcggctacg atccggttgc gcattacaca ggtcgggcac cactggaggc agccgcccag
      961 ggcattcctc tcggccccaa tgattgggcg atccgctgca acctggtcac gatcatcgat
     1021 caggtgatgc gagattttac ggccgaccac atctcaaccg gggaagcccg cgagttgatg
     1081 accacccttc aggaaaagct gggaacgccc caactccagt tctttcctgg tgtgagctat
     1141 cgtaaccttt taatctatcg aggtggggac aagccggcgc cgttttcgcg tgacaccgtg
     1201 accgttcccc ctcacgacct gaccgatcaa agcgtgattg acgcttatcc ccgtggtccc
     1261 ggcagtgact tgttgactcg gttaatgtgc gaaagcgcgg aaatctttgc caaccaccca
     1321 gtcaatgtgg cccggcaaaa ggcaggaaag ctgccggcta cgaatatctg gctgtgggga
     1381 cttgggggag ccccgcagtt accgccgttt caacaacgat atggggtcac cggggcgatg
     1441 atcacggcgg ttgaccttct tcgcggcctg gccgccttgt tcggatggcg acggattgat
     1501 gttcctggag caaccggtta catcgataca gattacgccg ccaagggacg ctacgctatt
     1561 gaagccctaa aagaagtgga cctagtatgc gttcacatcg aagccactga cgaggcctcg
     1621 cacgagggtg acgccgaggc aaaaatccaa gccctagagg cgatcgatca gaaaatcgtg
     1681 gcccccttgt gggaggccct tcaaaattat ggggattata ggattcttgt ttcacccgat
     1741 catcctacgc cactcaggtt gaaaactcac gcccacggct acgttccctt tgcgattgct
     1801 gggacgggga ttcccaccag tggcgcgacg ggctacaacg agcggacggc ggaagaatcg
     1861 gggattatct ttgaagaggg ttggcgtgtg atggagtatt tccttcgggg tgaatcgcca
     1921 ccttgagtgg ctgctcacga caggttcgtg accaccagca gggtacttga ttgggtgaaa
     1981 gagattcctt tttggaatac gcagttagaa acagttgagt caaagattgg aacaaggatt
     2041 gcgcacaatg gcagtgttag ttcaaaagtt tggtgggaca agtgtcgccg atgcagaaaa
     2101 aatcctagcg gctgcccgaa aagccattcg ggccaagctt cagggttatc aggttgtgac
     2161 cgttgtcagc gcaatgggtc acactagcga tcatctaatc gaattggcga agcaaatcga
     2221 ccctgagcct ccggcccggg aaatggatat gctgctttcg actggcgagc aagtcagcgt
     2281 tgccctcatg gccatggcaa tccaccgcct agggcatcgg gccatcagta tgacgggggc
     2341 ccagatgggc atttacacgg atagtaccca caccaaggcc cgcattcgat ccatctctac
     2401 agaccggatt cgacaactgc tcgacgaggg caatattgtt attgcggcag gattccaagg
     2461 gatcaccgac accgccgata ttacgaccct gggacgtggg ggaagcgaca ccacggcggt
     2521 tgccctagct gcggcgttac gggcggaaac ctgcgagatt tacacggatg tcgatggcgt
     2581 ctatacgacc gatccccgac tggtcccaga agcccgccga gtgagccgca tcagttatga
     2641 cgagatgctc gaattggcga cggcgggggc cggggtgatg cacaatc
//
LOCUS       DAOPTR010000531         2684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_30286_length_2684_cov_3.944085, whole genome shotgun sequence.
ACCESSION   DAOPTR010000531 DAOPTR010000000
VERSION     DAOPTR010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30286_length_2684_cov_3.944085"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1871
                     /locus_tag="PKI05_10295"
     CDS             <1..1871
                     /locus_tag="PKI05_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOA52635.1"
                     /translation="RIVKGDVPEILLDKRVVVLDLAMMVAGTKYRGQFEERIKAVMNE
                     VRRAGNVILFIDELHTLVGAGGAEGAIDASNVLKPALARGEIQCIGATTLDEYRKYIE
                     KDSALDRRFQVVMVEPNTKEETIAILKGLRDRYEKHHHVQITDDAITTAVELSSRYIT
                     GRCLPDKAIDVIDEAGARVHLKSLTRPPDLKEIDEEIERLNKEKEEAVANQDFEKAAK
                     LRDQADKLKKKKANMMHEWRERSASVDGIVDAEVVAEVVSKMTGIPLTRMTTEDSIRL
                     LKMEEELHKRVISQDEAIKSICKAVRRSRSGLKDPKRPVGCFIFAGPTGVGKTLLAKA
                     LAEFMFGDEDALIQIDMSEYMEKHNVSRLIGAPPGYVGYEEGGQLTEKIRRRPYSVIL
                     LDEIEKAHPDVFNMLLQVMEEGRLTDSFGRNVDFRNTILIMTTNAGAEAIKNEAAFGF
                     QKPDDDTSYENMKRRVIEQIEKVFRPEFLNRVDDIIVFRHLTQKDLFDVVELELGKVR
                     SRLAERGLSLVLTEEAKKLIIKRGTNLDFGARPLRRAIENYIEDPLAEELLKGEFEGK
                     DTIVVKVKDVGGKKQLVFEGVVGQPDLSSEGETSGEQQPEVAGVSGGEGSPSPSQE"
BASE COUNT          599 a          728 c          832 g          525 t
ORIGIN      
        1 aacggatcgt gaaaggtgac gtccccgaaa tcctgctcga taagcgggtc gtggtcttgg
       61 acctggccat gatggttgcc gggacaaagt accgcggtca attcgaggag cggatcaagg
      121 ccgtcatgaa tgaagtgcgt cgcgctggga acgtgatcct cttcatcgac gagttgcaca
      181 cgctggtcgg tgccggtggc gcggaaggcg ccattgacgc ctcgaacgtt ctcaagccgg
      241 ccttggcccg tggtgaaatc cagtgcatcg gtgccacgac gctcgacgaa taccgcaaat
      301 acatcgagaa ggacagcgcc ctcgatcggc ggttccaggt ggtcatggtg gagccgaaca
      361 ccaaagagga gacaatcgcc attctcaagg gcttgcgtga ccggtacgaa aagcatcacc
      421 acgtgcagat cacggacgat gccattacga cggcggtgga gctttccagc cgctacatca
      481 ctggccggtg tctgcccgac aaggccattg acgtgatcga cgaggccggt gcgagggtgc
      541 acctgaaatc cctcacccga ccgcccgacc tcaaagaaat cgatgaggaa atcgagcggc
      601 tgaataagga aaaagaggag gccgtggcca atcaagactt cgaaaaggcc gccaagctcc
      661 gcgatcaggc cgataagctc aaaaagaaga aagccaacat gatgcatgag tggcgggagc
      721 gctcggcctc cgtggacggc attgtggatg ccgaagtcgt ggccgaggtg gtctcgaaga
      781 tgacgggcat ccccctcacg cggatgacga cggaggattc catccgcctg cttaagatgg
      841 aggaggagct gcacaagcgc gtcatcagcc aggatgaggc catcaagtcc atttgcaagg
      901 cggtccgccg tagccgaagc ggtctgaaag accccaagcg gcccgtcggg tgcttcatct
      961 ttgccgggcc tacgggcgtg gggaagacct tgctggccaa ggccctcgcc gagttcatgt
     1021 tcggcgatga ggacgccctt atccaaatcg acatgagcga gtacatggag aagcacaacg
     1081 tcagccgcct catcggggcc ccgcctggct atgtgggcta cgaagaaggc ggacaactca
     1141 cggagaagat ccggcggcgt ccgtactcgg tgatcctcct cgatgagatc gagaaggccc
     1201 atccggacgt cttcaatatg ctgctccagg tcatggaaga aggccgcctg accgacagct
     1261 ttggtcgcaa tgtggacttc cgcaacacga tcctcatcat gacgaccaat gcgggcgccg
     1321 aggccatcaa gaacgaggcc gccttcggct tccagaagcc ggacgatgac accagctacg
     1381 aaaacatgaa gcgacgggtc atcgagcaga tcgaaaaggt cttccggcca gaattcctca
     1441 accgcgtcga tgacatcatc gtcttccgtc accttacgca gaaagacctc ttcgacgtgg
     1501 tcgaactgga gctcggaaag gtccgctcac ggttggccga gcgaggtttg tcgctggtcc
     1561 tcactgaaga ggccaagaag ctgatcatca agcgcggtac gaaccttgat ttcggtgcac
     1621 gtccgctgcg tcgggcgatc gagaactaca tcgaggaccc gcttgccgag gaactcctca
     1681 aaggtgagtt tgagggcaaa gacactatcg tcgtcaaggt gaaggacgtc ggcgggaaga
     1741 aacaactggt cttcgagggt gtcgtcggcc agccggactt gtcgtcggaa ggtgagacat
     1801 ccggtgagca gcagccggaa gtcgccggtg tctccggcgg cgaagggtca ccctcgccgt
     1861 cccaggaatg acggaaagac cgccgtcctc ttgaatgaga catgaacgcc gtcgcgggcc
     1921 ctggcaatgc gccgctaacc gccagggtct ttatttttga ccatggctcg tggcagagcg
     1981 ggcggcgcgt gatgctcgtc gcacgcatct tttacccctg tgtaagggcg taacccccgt
     2041 cgccaagagc cgattgaccc acctttagag gcgacacgcc agaaattgat tgccttccac
     2101 cataaagagg ggttatcatc gtaattttgg tcgttatctt agatttattg gaatgtgcac
     2161 ctccgtggcg gcatttcccg cccagggtgc ggatcacgcg atcatcggtc tctgggcaca
     2221 aggctgtttg gttttcccaa gagaattctt tgtggccctg cgcgaaccgg ggattgtctg
     2281 gcgataataa taacaataag catcgcgtgt atctacagtg attcggtctc atggtgcgtc
     2341 ccaacccacc ccggataagg acttttgtcc tgtggaagac ggctgtactt agggggtagg
     2401 aacgtgctag ggggtggatt cgcccgtgag acaatcgcag agggtggtta ttctcgacaa
     2461 ttcccaacgg tggcatgtgg cggggtaatg gcgtgttttg tcgccacagt tgcccagggt
     2521 gtgagtttgt tgttgggtgg ttaggttttc gagaaggaag cgagcgtacg gactatgtgt
     2581 attctggctt tgcagtacca agtcgccaaa gatgccccga ttttggtggc atataaccgg
     2641 gaggaggccc tggatcggcc aagttcggtg ccgcggatcc agtc
//
LOCUS       DAOPTR010000532         2675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_30434_length_2675_cov_3.122901, whole genome shotgun sequence.
ACCESSION   DAOPTR010000532 DAOPTR010000000
VERSION     DAOPTR010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30434_length_2675_cov_3.122901"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..1493)
                     /locus_tag="PKI05_10300"
     CDS             complement(18..1493)
                     /locus_tag="PKI05_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52636.1"
                     /translation="MKRFNVLACLAATVAVVNVGSSFLHGQELDFRAVRVEVDFGKKF
                     QVWDGFGFNYVEACQTRDYAANPQDYGGFSILDVTKKKEIIELVFGEEGLKVALIKMF
                     LDPFHQLRPGDPYDHETYTQNMRLFVREGLKTTRARGGDLTIITTLYGPPAYMTKQKV
                     LRGRDFDPQHKQDLVNYLVDWVRFLREKEGFPVKYISLHNEGEDWFRWTQAGLTDRDS
                     HDYNLFWPPEQVVEFVKLVDTALKQAGLEEVKVTPGETTNWYRFDHWGYADALAEDAE
                     ALRALGLITSHGFYSGTFGRWFGEHRSAGIDKLRGLRAELHAWVTSTSWGQMDAKNTK
                     EFHGNIYTAKVNGIIPWAGIQRPTKWTGGDPNPGCAFKVNEDGTYEIRRGYWMYKQLS
                     RAGQPGMVVAKATAMDSEIAVIAFASNGTPNPDAFVVINIGKQRLLKIRIVGSDAKAF
                     QVFRTTEDGKECYAPRGRLVCEENALVYEAPELSVTTFFAE"
     gene            1626..>2675
                     /locus_tag="PKI05_10305"
     CDS             1626..>2675
                     /locus_tag="PKI05_10305"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOA52637.1"
                     /translation="MRISQTQNRTPVIALLFFGCVVFAFTVAWAESPRPWERQYSGEE
                     ATGPNVIALWQFLPGQETVDNSTHGHDLRLRGQSRFAGDGVFGNCLECFASTGQRDAG
                     NGAVVRNHPSLSPTGAFTLELWIKPKPELAECDLAFLLDKKYLHYPRETPQANRDYCL
                     YLRRTGPGKFRLVAYLGFGNDSAEFTSREVVLESGRWVHIAFSYDAAGVGRFFLNGEP
                     VGRTVHPGRGAIVPGQYDLVIGDRYGSLYWGFPGWIDQVRILNTLPDWAAGQLEIEPS
                     PLARTAFLRMEPNASVTLVLRNDTNREVMNAEISWDLADHRAKQTVSKIVPREEVELK
                     VPVDTRLRPGHYSLHV"
BASE COUNT          597 a          748 c          712 g          618 t
ORIGIN      
        1 aaaagacatt cgccgggtta ttcggcgaag aacgtggtca ccgatagctc cggcgcttcg
       61 tacaccaaag cgttttcctc acagacgaga cgtcctcgag gcgcgtaaca ctctttaccg
      121 tcctcggtgg tgcgaaagac ttggaacgcc ttggcatcgc ttcctacaat ccggattttc
      181 aagagccttt gtttgccaat gttgatgacg acgaaggcat ctggattagg agtgccgttg
      241 gaggcgaagg cgatcacggc gatttcgctg tccatagcag tcgctttggc aactaccatc
      301 cccggttgac cagcccgcga cagttgtttg tacatccagt acccccggcg aatttcatat
      361 gttccgtcct cattaacttt gaaggcacag ccagggttgg ggtcaccgcc cgtccacttt
      421 gttggccttt gaataccggc ccaagggata atcccgttga ccttggccgt gtagatattg
      481 ccgtgaaact ccttggtatt cttggcgtcc atttgtcccc agcttgtgga agttacccaa
      541 gcgtggagtt ccgctcgtaa accgcggagc ttatcaatcc cagcactccg gtgttcgcca
      601 aaccaacggc cgaaggttcc gctataaaag ccgtgcgaag tgatcaatcc caatgcgcga
      661 agggcctcgg cgtcttcggc gagggcgtcg gcatagcccc aatggtcaaa gcgataccaa
      721 ttcgtggttt cccccggtgt gaccttaact tcttccagac cggcctgctt taacgcggta
      781 tcgaccaact tcacgaattc gacaacctgc tctggtggcc agaacaagtt atagtcgtgg
      841 ctgtcacgat cggttagacc agcctgagtc cagcggaacc agtcctctcc ttcgttatgt
      901 aaagagatgt atttgacagg gaagccttcc ttttcacgaa gaaagcggac ccaatcgacc
      961 aaatagttca caaggtcctg tttgtgctgg ggatcgaaat cccggccgcg gagaaccttt
     1021 tgttttgtca tgtaggctgg tgggccgtaa agcgttgtga tgatcgtcaa gtctccgccc
     1081 ctggcccgcg tggtcttgag gccctcacga acaaaaagcc gcatattctg ggtgtaagtc
     1141 tcgtgatcat acgggtcgcc cggccgaagc tggtggaacg gatccaagaa catcttgatc
     1201 aaagcaacct taagaccctc ttcgccaaag acgagctcaa tgatttcctt tttcttcgtt
     1261 acgtcgagga tcgaaaaccc gccgtaatct tgcgggtttg cagcatagtc gcgggtctgg
     1321 cacgcctcga catagttaaa tccgaagcca tcccagactt gaaatttctt gccaaagtcg
     1381 acttccacac gaacggcgcg gaaatccaat tcctgcccgt gtaggaacga gcttcctaca
     1441 ttgaccacag ctaccgtggc ggcaagacag gctaacacat taaaccgttt catggcactg
     1501 ccactcccat tatcaccttg ttgaatgcta aacgatgcaa caatatccta atgggcaatc
     1561 gctctcaatt tacaggacac aggccacaac ccagatcaca gcttgactta attagggagg
     1621 aacacatgcg aatatctcaa acgcaaaata ggactccagt cattgctttg ttgttctttg
     1681 gatgcgtcgt gttcgccttt accgtggcat gggcggagtc tccacgtccc tgggagcggc
     1741 aatattctgg cgaagaggcc acaggcccaa acgtgattgc attgtggcag tttttgcccg
     1801 gccaggaaac agtggacaat tccacacacg gccatgactt gcgactccgg ggccaatcac
     1861 gctttgccgg agacggggtc ttcggcaatt gtctagaatg ctttgcgagc acaggccaaa
     1921 gggacgccgg aaacggcgcg gtggtaagaa accatccgtc gttaagccca accggggcgt
     1981 tcactctgga attatggatc aagccgaagc cggaattggc cgagtgcgat ctggccttct
     2041 tgttggacaa aaagtacctc cactacccac gggaaacgcc ccaagcaaac cgtgattact
     2101 gcttgtacct acggcggacg gggccgggca agtttcgctt ggtggcgtat ctcggttttg
     2161 gcaatgattc ggcggaattc acttcccgcg aggttgtgct ggagtctggg cgctgggttc
     2221 atatcgcctt ttcctacgat gcggcgggcg tcggacgatt ctttctcaac ggagaacccg
     2281 ttggccgaac ggttcaccca gggcgcgggg ccatcgtgcc tggtcaatat gatttggtaa
     2341 ttggcgatcg ctacgggtct ctttactggg gctttcccgg ctggattgat caggtcagaa
     2401 tcctcaacac gctgccggac tgggcagcgg ggcaactgga gatcgagcct agccccctcg
     2461 caagaacggc cttcctccgc atggagccca acgcctctgt cacgctcgtc ctgcgcaacg
     2521 atacaaaccg ggaagtgatg aatgccgaaa tctcctggga tctggctgac caccgggcga
     2581 agcaaacggt ctccaagatt gttccacgtg aggaagtcga actgaaggtc cccgtggaca
     2641 ctcggttgcg cccaggtcac tattcgctcc acgtt
//
LOCUS       DAOPTR010000533         2675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_30436_length_2675_cov_3.053817, whole genome shotgun sequence.
ACCESSION   DAOPTR010000533 DAOPTR010000000
VERSION     DAOPTR010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30436_length_2675_cov_3.053817"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /locus_tag="PKI05_10310"
     CDS             complement(<1..1388)
                     /locus_tag="PKI05_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52638.1"
                     /translation="MDKFPQSLTQRKKQIMKYYVIHVGVIALLLSCVAPHDIATILAE
                     ERAGRTFFVDSEGGHDDADGQSPERAWKSLERVNAADLKPGDTVRFKCGGVWRGSLIP
                     VSGDEQAPVTYTSYGEGPKPLLLGSRPRNRPEDWVHVGPKLWATLPVEYRLGRERLDL
                     RNSTCRHHQEAGAQVELTHEGDADNRVVRVTCIASGSAANHIQLWGPAVAVQKGEHLV
                     FTFRARSSKPFMLPPVNLLKAGFPYSRYAQSTLPKETVGPEWATYQIPLRASESAEDG
                     GLHINLGGIIPAGAIFEFQAQTLHEATPSVPDPLDTDVGNIIFDNGAICGWKKWSLDS
                     LEKPYDFFYDGTSQRVFLFSEVVPTATHRSIELALTRHVVNQSGKHHVIYDGLAVMYG
                     AAHGFGGGGTHHIIIRNCDLAYIGGGHQYTRPDGKPVRYGNAIEFWGAAHDNLVEGCR
                     IWEVYDAALTNQG"
     gene            complement(1576..>2675)
                     /locus_tag="PKI05_10315"
     CDS             complement(1576..>2675)
                     /locus_tag="PKI05_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52639.1"
                     /translation="IAAYEASIKARKERGEPIPPNSAPPSDLRPGPAADRNRPGYCYA
                     SVIRPLEGFSIKGVLFHQGFNNCFNGSAGARMYYQVFGRMITAWRAAFRDPQLAFCII
                     ALPTAGEPQTAENFVQAMYDVGPYIREAQYRTFRNFYDAGDKAIGYVSTYDLRKSWYH
                     PQIKIPVGERAAKWALVTQYGILSGRDAEQYWLPPSITRVEAVDGTLRLYFSTEVRTK
                     DDADGKLLGFAIAGEDRRFFPADVRYYTEKSDDGQNQPREQRHILVLSSPFVPQPVHY
                     RYAWARNPMGNIVNPRGVPLATQRSDNWILEETPEKIPVPQGMSPETAQRYIGNRLRE
                     LLKQADLERRYLEAKRTLQELAPLVERGKTN"
BASE COUNT          541 a          755 c          781 g          598 t
ORIGIN      
        1 ccttgattgg tcagtgcggc gtcgtaaact tcccagattc ggcaaccctc gaccaagttg
       61 tcgtgggcgg ccccccaaaa ctcgatggca ttgccataac ggacgggctt cccatcgggg
      121 cgtgtgtact ggtgtccgcc cccaatgtac gccagatcgc aattgcggat gatgatgtga
      181 tgcgttccgc cgcccccaaa gccgtgtgcc gcaccgtaca tcacagccaa gccgtcgtaa
      241 atcacgtggt gcttaccgct ttggttgacc acgtgccgcg tcagcgccaa ttcgatgctg
      301 cgatgcgtcg cagtgggaac gacctcgcta aagaggaaga cccgttgcga agtcccgtcg
      361 taaaaaaagt cgtagggttt ctcgagcgag tcaagcgacc attttttcca accacaaatg
      421 gcaccattgt cgaaaatgat gtttcccacg tctgtgtcca aagggtcagg cacactgggc
      481 gtagcttcgt ggagggtctg agcctggaac tcaaagatgg cacccgcggg gatgatccca
      541 ccgagattaa tatgcaagcc cccgtcctcg gcagactcgc ttgcccggag cggaatttgg
      601 tacgttgccc actccggccc gactgtctcc tttggtaatg ttgattgggc atagcgggaa
      661 tatgggaagc cggctttaag aagattcacc gggggtagca taaacggttt gctgcttctg
      721 gcgcgaaagg taaaaacgag atgttcaccc ttttggaccg ccactgctgg cccccatagt
      781 tgaatgtggt ttgctgccga gcccgaggcg atgcaggtga cgcgaacgac ccggttgtcg
      841 gcgtcgccct catgcgtcaa ctctacctgg gcccctgcct cttgatggtg cctacaggtg
      901 ctattccgca aatcgagtcg ttcacgtccc aagcggtatt ccacaggcag ggtggcccaa
      961 agtttggggc cgacgtggac ccagtcttct ggccgattgc ggggccgtga cccaagaagc
     1021 aggggctttg gcccctcgcc gtaagaggtg taggttacag gggcctgctc gtcgccgctt
     1081 accgggatga gcgatccccg ccacaccccg ccgcacttga accggactgt gtcgccgggt
     1141 ttcaaatcgg cagcgttaac gcgctcaagg gatttccagg cccgttctgg agattggccg
     1201 tcggcgtcgt cgtgtccgcc ctccgagtcc acgaaaaagg tccgtccggc tcgttcttcg
     1261 gccaagatcg ttgcaatgtc atggggtgct acgcaagaaa gcaacagcgc gatgactccg
     1321 acatgtataa cgtaatactt catgatctgt ttctttctct gggtgaggga ttgagggaat
     1381 ttgtccatcc aacttccttc gggttttcgg catcacaaac gcgtgtggat gcttccggtg
     1441 tttaagctta gggtgtccgg ttcggcttcc cgtacaatgg caagctacac gtcgaaagcc
     1501 taggaaagac aatttccctg ttaccgaacc agtaacgaga ctccggcgca cgaaaacaat
     1561 tgaggttcac tctaatcagt tcgtttttcc ccgttccacc agcggggcta gttcctgaag
     1621 tgtccgtttt gcctccagat accgtcgctc caggtccgcc tgttttaaaa gttcacgaag
     1681 ccggtttcca atataacgtt gggcggtctc cggcgacatg ccttggggaa cggggatctt
     1741 ttcaggggtc tcctccaaaa tccagttgtc gctccgctgc gtggcaaggg ggacgccccg
     1801 gggattcacg atgttgccca tcggattgcg cgcccaagca tatcggtaat ggaccggctg
     1861 aggaacaaat gggctgctca gtacgaggat gtggcgctgt tctcgcggct ggttctgacc
     1921 gtcgtcagac ttttcggtgt agtacctgac atcggcgggg aaaaaccggc gatcttctcc
     1981 cgctatagcg aaacccaaaa gcttcccgtc ggcatcatcc tttgtgcgga cttccgtgct
     2041 gaaataaagc cgaagcgtgc cgtcaacggc ttccacccgg gtgatggaag gcggtagcca
     2101 atactgctcg gcgtctcgcc ccgaaagaat cccgtattga gtcaccaatg cccattttgc
     2161 agcccgttct cccacgggaa tcttgatctg aggatggtac caacttttgc ggaggtcgta
     2221 ggtgctgacg tagccaatgg ctttatctcc ggcatcgtag aagttgcgga aggtccggta
     2281 ctgtgcctcg cggatgtagg gcccaacgtc gtacatcgcc tgaacgaaat tttctgcagt
     2341 ttgcggctcg cccgccgtgg ggagcgcgat aatgcaaaag gccaactgtg ggtcgcgaaa
     2401 ggcagcacgc caggccgtga tcatcctgcc gaagacctgg taatacatcc gcgctccggc
     2461 ggacccgttg aaacaattgt tgaatccctg atgaaagagg acgcccttga tggaaaagcc
     2521 ttcaaggggg cggatcacgc ttgcataaca atagccggga cggttgcgat cggccgcggg
     2581 gccaggtcgt aagtcgctcg gtggggcgct gttcggaggg atcggctctc cgcgttcctt
     2641 ccgggcctta atggaggcct cataggcagc gatgc
//
LOCUS       DAOPTR010000534         2673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_30460_length_2673_cov_3.362490, whole genome shotgun sequence.
ACCESSION   DAOPTR010000534 DAOPTR010000000
VERSION     DAOPTR010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30460_length_2673_cov_3.362490"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..830)
                     /locus_tag="PKI05_10320"
     CDS             complement(231..830)
                     /locus_tag="PKI05_10320"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOA52640.1"
                     /translation="MCIVFHKKRAKVTYRNTDNSPQGERRGSGWLLAILALSLVLSAG
                     CVQRRLIIRSQPPGAMVYVDDYQIGPTPIATSFTYYGTRKIRLVKDGYQTLTVYERID
                     PPWYQIPPIDFFAENVLGREVRDTRVLEYQLQPQPISPTQQLLDRAETLRRQTGGSPP
                     LPAAAPIQQTQQPGMPSGAPPTEGSTAPAFIIEPPRTSY"
     gene            1107..1400
                     /locus_tag="PKI05_10325"
     CDS             1107..1400
                     /locus_tag="PKI05_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52641.1"
                     /translation="MRLMILGGSGLVGVAQLSEAVEAAEWLNAELIKAALHTATPEEE
                     GFVEYVVGLVQQGQLPAQILQSAYLWAQKRPKYKFQYFKRAIIYLAAQKGIRI"
     gene            complement(1471..1920)
                     /locus_tag="PKI05_10330"
     CDS             complement(1471..1920)
                     /locus_tag="PKI05_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009093617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOA52642.1"
                     /translation="MATKKNSSTSLASEEKQALDSPNAQAVETRRKRILIVDDDVEIV
                     ESIRVALESLGYQILIARDGNQGLALADAEDPDLVILDMMMPKRSGFLVLEKLRQTRK
                     VPVRVIMITANEGSRHKAYAEMLGVDDYIRKPFAMDRLIESVQRLLE"
     gene            complement(2108..>2673)
                     /locus_tag="PKI05_10335"
     CDS             complement(2108..>2673)
                     /locus_tag="PKI05_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52643.1"
                     /translation="MERCALVREKLPAPALTTDVMVGFPGETDEDFEATCRVVETVGF
                     AKVHIFRFSPREGTPAAEFPDQIPEKTKKDRARTLDAVAEKTRRQYINSLFGRQLQVL
                     WEDLASAPGDNSPQWLLGMSDRYIPVRALASRGKQGELDGVTVEGFDGTFLLDRDLFA
                     VTSPPEMCQNNAQVGCVGCNFDDFPTS"
BASE COUNT          570 a          724 c          748 g          631 t
ORIGIN      
        1 tctcgttatt gccggactca atccgattgc gattctcgaa gagatgggct accaagtgta
       61 ctctcgggcc ttaagcggcc tgatggactt tagtcggctt atcactgtgg acgaactgcc
      121 cacggccctt cagcgctttt tgaaatgaag taagggtgcc cgcgaggatc gttcgaatat
      181 gctcgttgag gtctgattct gcacagcgtg ctcttcgact tcactcatga ctaataggaa
      241 gtccgaggcg gttcaatgat aaaggccggc gcggtgctac cctcggtcgg tggggcaccg
      301 gatggcatcc ccggttgctg tgtctgttgg atcggtgctg ccgcgggtaa gggcggagaa
      361 ccgcctgtct gtcgccgcaa tgtctccgcc cgatcgagga gttgctgcgt gggcgagatc
      421 ggttggggtt gtaactggta ttccaggacc cgtgtgtcgc ggacctctcg tcccaggaca
      481 ttttcggcaa aaaagtcaat cgggggaatc tgataccagg gcgggtcgat ccgctcatag
      541 acggtcaagg tttgataacc atctttcaca agccggattt ttcgcgtccc gtagtacgtg
      601 aatgatgtgg caatcggcgt tggaccgatc tggtaatcat cgacatagac catggccccc
      661 ggcggttgac tgcggataat gagtcgccgt tgcacacacc cggcggaaag gacaagagag
      721 agggccaaaa tcgcgagcag ccacccactg ccgcgtcttt caccctgagg tgagttatcc
      781 gtgttccgat aggttacctt ggctcgcttt ttgtgaaaaa caatgcacat gaccacctga
      841 aaatgattcg aggactctgg ggataaccga acgtgcccag cgacggtcgg agtataactt
      901 tttttgcaaa tcccgctagc ccaaatcttg cgatacgttg ggccagtgtt gcccgtctta
      961 ctaacgattt cccttgccgt aggattcaaa aatcggtagg atgccccccg ttagcgttcg
     1021 ggagagaatg tctccgggtt agcgccccag ttgaaacgcg agatcaagaa gaaagaggtt
     1081 cccccattat gcgccggcga gagtttatga ggttgatgat actgggtggc agcgggctag
     1141 tgggagtcgc gcaactctcc gaagcggttg aagccgccga gtggctcaat gccgaactta
     1201 tcaaagcggc ccttcatacg gccacaccag aggaagaagg ctttgtggag tacgtggttg
     1261 gattggtgca acaaggacaa ctgcccgccc aaattctcca gtccgcctat ctttgggcgc
     1321 aaaagaggcc aaagtacaag ttccagtatt tcaaacgagc gattatctac ttggctgcgc
     1381 aaaagggaat ccggatttga tgatcccctc gatggcctga atcctcccaa ggggtgtgcc
     1441 gggaccgcgg tgggttatcc atccgcaggg ttattcgagg agccgctgca cgctttcgat
     1501 caaacgatcc atggcaaacg gcttgcggat gtaatcatcc acgccgagca tttccgcata
     1561 ggctttgtga cgacttcctt cgttggccgt gatcatgatg acccgcacag ggactttgcg
     1621 ggtttgccgc agcttttcca gcacaaggaa accactgcgt ttgggcatca tcatgtcgag
     1681 gatcacgagg tctgggtctt cggcatcagc tagggccagg ccttgattac cgtcgcgcgc
     1741 aattaagatt tgatagccga gcgactccag cgcgacgcga atcgactcga cgatctccac
     1801 atcgtcatcg acgatgagga tccgcttacg gcgggtttcg acggcctgtg cgtttggcga
     1861 atccaatgcc tgcttctcct cggaagccaa cgatgttgac gaattctttt ttgtggccat
     1921 acgtttgacc ctcaccttgg gtgggctaag gcctattttc ggcacctcag ggttactcac
     1981 cacccggcgg gaacctcccc ctaattgctc tgactgtgcc gaaacacctt tcttcagtta
     2041 cctgcccccg acagcgtccg cgagacatcc cgcgaaggcg gaactctttc ttctgattgc
     2101 ctgctactta tgatgtcgga aaatcgtcaa aattgcaacc gacgcatccc acttgggcgt
     2161 tattttgaca catttcggga ggtgatgtga cagcgaacaa gtcccgatcg agcaggaacg
     2221 taccgtcaaa tccctccacg gttaccccgt ccaattcacc ttgcttcccg cggctggcca
     2281 aggctcttac agggatgtag cggtcgctca ttcccaaaag ccactgagga gaattgtcac
     2341 cgggagcgct agccaggtct tcccagagta cttgaagttg acgtccaaaa agggagttga
     2401 tgtactgcct tcttgtcttc tcagcaaccg cgtcgagtgt gcgtgcccgg tcttttttcg
     2461 tcttttcggg gatttgatcg ggaaactcgg cggctggtgt tccctcgcga gggctgaaac
     2521 ggaagatgtg aaccttggcg aagcctaccg tttccacgac ccgacatgtt gcctcgaaat
     2581 cttcatctgt ttcgccagga aagccgacca tgacatctgt tgtcaaggcc ggagcaggaa
     2641 gtttctcgcg gaccagggcg cagcgctcca taa
//
LOCUS       DAOPTR010000535         2671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_30479_length_2671_cov_2.801988, whole genome shotgun sequence.
ACCESSION   DAOPTR010000535 DAOPTR010000000
VERSION     DAOPTR010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30479_length_2671_cov_2.801988"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PKI05_10340"
     CDS             complement(<1..260)
                     /locus_tag="PKI05_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52644.1"
                     /translation="MDAPLFVLILASIFTAGGLWWALPWGKARRRSVGWVLVAAGLAM
                     LSAMTPPVGSLLPRSLLTLMSIVAVGSAVAMITSSRPLYAAL"
     gene            complement(294..878)
                     /locus_tag="PKI05_10345"
     CDS             complement(294..878)
                     /locus_tag="PKI05_10345"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HOA52645.1"
                     /translation="MKPDSSEIRWVDESPLNMGERSYLPLFWDGLTTTFRHLIGPKLT
                     VQFPEERHQFPDPLIYRGVHRLNRDEQGRVKCVACFLCATACPAHCIDIVAGPSPWPD
                     REKYPVVFNIDMLRCIFCGMCEEACPVDAIELTSLYDLSGKSREEMIFDKEKLLSIYD
                     QTKDQEPPGWLRGKREIEDLIELPQDDQDLTQDK"
     gene            complement(937..2202)
                     /locus_tag="PKI05_10350"
     CDS             complement(937..2202)
                     /locus_tag="PKI05_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOA52646.1"
                     /translation="MISFAVVTCIKIAVLLAALMTAAAYLVLVERWIAAWVQDRLGPN
                     RVGIPLTNIRIFGLGQPIADGLKLIFKEEFVPGHVDRILYNVAPVVTFVAAVAVYAVV
                     PLGAVDLGISGWAPVDLTVVPAFDAALIFLFAANGAAVYGIILAGWSSNNKYSLFGAM
                     RGAAQLIAYEIPLSLALIALACYAGTLRIDEIVLQQAESGVWNIVLHPLAFVVFFVAA
                     TAEAGRLPFDLPETEQELVGGFHTEYSGMRLMTFLVSEFLHMITAAVVIVLLFLGGWH
                     LWFLTGSEPVAGIGGAIIRIVVFVAKVLAVVFFFMWMRWSWPRFRFDQLMALAWKPLI
                     SWGIVQVTILVVWYAWWEPLVVSQGWSSLPVLALINWVTFILTWVITAAIAQPHYDNS
                     PRRDLVRLAQGVPEGSPYMESPTVQPCDA"
     gene            complement(2291..>2671)
                     /locus_tag="PKI05_10355"
     CDS             complement(2291..>2671)
                     /locus_tag="PKI05_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOA52647.1"
                     /translation="IDAEQADALRRATALIVADVFPTPLSDRADLIFGMAFFAEREGS
                     YVNRQHRLQWSARAIRPPFGSRCLGSVFWELGGMSGLYQPREVLAEIARTIPYFSAAS
                     GEIPECGVDLRVNQLAEQTVSTGQ"
BASE COUNT          699 a          802 c          654 g          516 t
ORIGIN      
        1 caaagggcgg catacaacgg acgactgctc gtgatcatgg ccaccgccga gccaacggcc
       61 acaatagaca tcagtgtgag aagagagcgt ggtaacaggc tccccacggg cggagtcatg
      121 gccgaaagca tcgccagccc ggcagccaca agcacccagc ccacagaacg tctccgtgcc
      181 tttccccacg gcagcgccca ccacaggcct cccgccgtga aaatgctcgc caagatcaaa
      241 acgaagagtg gtgcgtccat aacattatcc acaaacaccg aagaaacgat cactcacttg
      301 tcctgtgtga ggtcctgatc gtcttgcgga agctctatca aatcctcgat ttcccgtttt
      361 ccccgcaacc agccgggagg ctcttgatcc ttggtctgat catagatgct caataacttc
      421 tccttgtcaa agatcatttc ctcacggctc ttgccagaaa gatcgtaaag gcttgtcagc
      481 tcaatggcat ctacgggaca ggcctcctcg cacataccgc aaaagataca ccggagcatg
      541 tcgatgttga agacgaccgg atatttctcc cgatctggcc aaggactagg accagcgaca
      601 atatcgatac agtgagcggg gcaagccgtg gcgcaaagaa aacaggcaac acatttcacg
      661 cggccctgct catcccggtt caaacggtga acaccacgat aaatcaacgg atcagggaat
      721 tgatgccgtt cctccggaaa ttgaaccgtc aacttcggcc caatgaggtg ccggaatgtt
      781 gtcgtcagtc catcccaaaa taggggcaaa tatgatcttt cccccatatt gagtggtgac
      841 tcatcaaccc agcggatttc cgagctgtct ggtttcatag gtcgttgatc tcatccaaag
      901 caaagatctc tctttcgata aagccttctt tgctttttac gcatcgcacg gctggaccgt
      961 tggggactcc atataaggtg aaccctccgg cacaccttgg gcaagacgca ccaagtcgcg
     1021 ccgcgggctg ttatcgtaat gaggctgggc aatcgcagcc gtgatgaccc aagttaagat
     1081 aaacgtcacc caattgatta gcgcgaggac cggcaacgac gaccagccct gcgacacaac
     1141 caagggctcc caccatgcgt accataccac caagatcgtc acctgaacga ttccccaaga
     1201 aatcaggggt ttccacgcaa gggccattag ttgatcaaaa cgaaatctgg gccagctcca
     1261 gcgcatccac atgaaaaaga acaccaccgc caggaccttg gccacaaaca caacgattct
     1321 tatgatcgct ccaccgattc ctgctaccgg ttcggacccg gtgaggaacc aaaggtgcca
     1381 acctccaagg aaaagcaaga caatcaccac ggccgcagtg atcatgtgga ggaattcaga
     1441 gaccagaaac gtcataagcc gcatgccgct gtattcggta tggaatccgc ccacaagctc
     1501 ttgctctgtt tctggcaagt cgaaaggcaa ccgccctgcc tcggcggtgg cagccacgaa
     1561 aaagaccaca aatgccaacg ggtgaagaac gatgttccat actccggact cagcttgttg
     1621 gaggacgatt tcatcaattc gaagtgtccc cgcataacat gccaacgcga tcagagccag
     1681 actcaaagga atctcgtaag cgatcaattg cgctgccccc cgcatcgcgc caaagaggct
     1741 gtacttgtta ttgctgctcc aaccggcgag gatgatcccg taaaccgcag ctccgttggc
     1801 ggcaaacaga aagatcaatg cggcatcaaa cgcgggaacg accgtcaaat cgacgggcgc
     1861 ccatccggag atcccaaggt ccaccgcccc aagcggcaca acggcataga cggcgacggc
     1921 tgccacgaat gtcacaaccg gtgcgacatt gtaaagaata cgatcgacgt gacctggaac
     1981 aaactcctcc ttgaaaatga gtttcaaacc gtccgcaatc ggctgtccta gcccaaaaat
     2041 ccgaatattc gttaatggga tgcctactcg gttgggacca agccggtcct gaacccaagc
     2101 ggcgatccag cgctcgacaa ggaccagata agcggcggcc gtcatcaagg cagccagcaa
     2161 cacggcaatc ttgatgcatg tcacaacggc aaagctgatc atcttcagtc ctgtttgcta
     2221 tcagggcttc ctaggtttgc catgtcactt tttcgctaca cgtccaaatt ggtaacgatt
     2281 ctcactattc ttactgccca gtcgataccg tttgctcggc caattgattc actcgaaggt
     2341 ccacgccgca ttccgggatt tcgcccgatg ctgctgagaa ataagggatg gtccgtgcga
     2401 tttcggccaa gacttcacgc ggttgataaa gcccgctcat cccgcccaat tcccaaaaaa
     2461 ctgagcccag acagcggctg ccaaatggcg gtcgaatcgc ccgcgccgac cactgaagcc
     2521 ggtgttgtcg gttcacatag gaaccttctc gctcggcaaa aaacgccata ccaaagatca
     2581 gatcggcgcg gtcgctgagg ggcgtgggaa atacgtcggc gacaatcaac gctgttgccc
     2641 gccgcagcgc gtccgcctgt tctgcgtcaa t
//
LOCUS       DAOPTR010000536         2666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_30556_length_2666_cov_2.554960, whole genome shotgun sequence.
ACCESSION   DAOPTR010000536 DAOPTR010000000
VERSION     DAOPTR010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30556_length_2666_cov_2.554960"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PKI05_10360"
     CDS             <1..338
                     /locus_tag="PKI05_10360"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOA52648.1"
                     /translation="PLSEVTGVVTFYSFFNTIPRGRHMIRVCLGTACYVRGGKQVLAA
                     LKEELGIDVGETTADRNFSLDVGRCFGACGLAPVIMIDDDVHQRVKVSQLPRLLEQYR
                     DKQDTTSKT"
     gene            434..>2666
                     /locus_tag="PKI05_10365"
     CDS             434..>2666
                     /locus_tag="PKI05_10365"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOA52649.1"
                     /translation="MAEESPKRNRVSSVNDLNAWRNQLLAEQKRQSDLGIKELRICTG
                     AGCIASGSLHTREEIVRQLQDRDLANRWKVISTGCMGPCFGGPVVMIGDVVYERVRAE
                     DAATIVVEHLGQGKIVDRLTHRRADGRHVAHLKDIDFFKRQVKIALRNCGVIDPGCIE
                     DYIARDGYQALAEVLEKRDPDAVIETLRESGLRGRGGAGFPTWRKWAFARQQEAPTKF
                     VVCNADEGDPGAFMDRSILEGDPHSVIEGMAIAAATIGAQKGFIYVRAEYPLAVQRVQ
                     VALSQAREVGLLGENILGLGFDFDIEIRMGSGAFVCGEETALMTSIEGNRGEPRPRPP
                     YPVEKGLWGHPTLINNVETLANIPIIILKGGAWYAQWGTPKSRGTKVFALAGGVKHAG
                     LVEIPIGMPLGDLIYDIGGGTASGKPFKAAQIGGPSGGCIPRQHLNVPLDYESLAELG
                     AIMGSGGLIVMDDDSCMVDVARFFLEFVQEESCGKCVPCRVGTKRMLEIMERICAGQG
                     EEDDVERLIELGEMIKETSLCGLGQTASNPVLSTIRHFGQEYVEHIRDRHCRAGVCSA
                     MVAAPCESACPAKVDIPGFVALVAEKRYAEAIRLHRERNPFAAVCSRVCFHTCEDKCR
                     RATIDAPVAIRAIKRFMVDQEITLQVPEIREDPKNAKRKIAIIGAGPAGLTCAYFLAR
                     LGYRPEVFEAEQRPGGMLVQAIPAYRLPREVVAREVRMIEQMGVEIITGIALGRDFTL
                     TELR"
BASE COUNT          548 a          666 c          829 g          623 t
ORIGIN      
        1 agccgctcag tgaggtcacg ggcgttgtga ccttttactc cttcttcaat acgatccctc
       61 gaggacgtca catgatccgt gtctgccttg gtacggcgtg ttacgtccgt gggggtaagc
      121 aggttctggc ggccctcaaa gaagagctgg gaattgatgt gggtgagacg acagcggacc
      181 ggaacttctc gctcgatgtt ggacgctgtt tcggcgcttg tggcctcgcg cccgtcatta
      241 tgattgatga cgatgtccac cagcgggtca aagtctcgca gcttccccgc cttcttgagc
      301 aatatcgcga taaacaggat acgacatcga agacttgaat gtgcttcggt gttgcgaagg
      361 ttggcaggtc ttgcttccac cagttgcctt cttttggagt gatgacattt gtttttggag
      421 tcaagcagtg accatggctg aagaatctcc aaagcgaaac cgggtgtcca gcgtcaacga
      481 tttaaatgcg tggcgcaacc agttgctcgc tgagcagaaa aggcagtcag accttgggat
      541 taaggagttg cgaatctgca ccggagcagg ctgcatagcc tccggctctc tgcacacgcg
      601 cgaggaaatc gtacgacagt tgcaggaccg agacttggca aatcgctgga aggttataag
      661 cacgggttgt atgggcccct gttttggcgg tccagttgtg atgatcggcg atgtggttta
      721 cgagcgcgtt cgcgccgagg atgccgccac gattgtggtg gaacatcttg ggcaggggaa
      781 gattgtggac cgattgacac accgtcgggc ggacggtcgc cacgtagcgc atcttaaaga
      841 catcgatttt ttcaaacggc aggtcaaaat cgccctccgc aattgtggcg tgatcgaccc
      901 tggatgcatc gaggactaca ttgcccgcga tggatatcag gccttggctg aggtacttga
      961 aaaaagagac ccggatgctg tcattgagac ccttcgtgaa tcggggttga gggggcgtgg
     1021 cggggccgga tttccaacct ggcgaaaatg ggcatttgct cggcagcaag aggcgcccac
     1081 caagttcgtg gtctgtaacg ccgatgaggg agaccccggg gcgtttatgg atcggagtat
     1141 ccttgaggga gaccctcata gtgtgatcga aggaatggcc attgcggcgg ccacaatcgg
     1201 tgcgcaaaag gggtttatct atgtcagggc ggaatatcct ctggcggttc agcgggtgca
     1261 ggtggccttg tcccaggctc gagaggttgg tcttttgggg gagaatatcc tgggtctggg
     1321 ttttgatttt gatatcgaaa ttcgcatggg ttcgggggca tttgtctgtg gcgaagagac
     1381 tgccctgatg acttcgatcg aaggtaaccg tggcgagcca aggccgaggc ccccataccc
     1441 ggtcgaaaaa ggactctggg gccaccccac gctaattaac aatgttgaaa ccctggccaa
     1501 tattcccatc attatcttga aagggggagc atggtacgcc cagtggggta cgcctaaaag
     1561 ccgcggcacg aaggtctttg ccctggctgg aggggtcaag cacgccggtt tggtcgagat
     1621 ccctatcggt atgccgctgg gcgacttgat ctacgacatt gggggcggga cggcatccgg
     1681 aaaaccgttc aaggcggctc aaattggtgg tccttcaggc ggatgtatcc ctcgccaaca
     1741 tctcaacgtt cctctcgact atgaatcgct tgccgagctc ggtgccatta tgggctcggg
     1801 aggtttgatc gtgatggatg acgatagctg catggtggat gtcgcccgct ttttcttgga
     1861 gtttgtccag gaagaatctt gcggcaagtg tgtgccgtgt cgcgttggca caaagcggat
     1921 gctcgagatc atggaaagga tatgtgccgg ccagggtgaa gaggatgacg tcgaacgcct
     1981 tatcgagtta ggggagatga tcaaggagac ttccctttgc ggcttgggac agacggcttc
     2041 gaaccccgtc ctttccacaa ttcgccattt tgggcaggaa tacgtcgaac atatccgcga
     2101 ccgccattgt cgggcgggtg tgtgttccgc catggtggcg gcaccgtgcg aaagcgcttg
     2161 ccctgccaag gtggatattc caggcttcgt ggccctggtg gctgaaaaac gctacgctga
     2221 agcgattcgg ttacaccgcg agcgaaatcc ctttgcggcc gtctgttctc gagtttgctt
     2281 ccacacctgc gaggacaaat gccgtcgtgc cacgatcgac gctcccgttg ccatccgtgc
     2341 catcaaacgg ttcatggtgg atcaagagat cacccttcag gtcccagaaa tccgagaaga
     2401 tcccaagaat gccaaacgaa aaatcgctat catcggtgct ggtccggcgg gtttgacgtg
     2461 cgcttatttt ctggcccgtc tgggatatcg tccggaagtc ttcgaggctg agcaacgccc
     2521 gggtggtatg cttgttcaag ccattcccgc ttatcgactt cctcgtgagg ttgtcgctcg
     2581 tgaggtgcgg atgatcgagc agatgggcgt cgagattatc acgggcatcg cccttggccg
     2641 tgacttcacg ttgacggaac tccgtg
//
LOCUS       DAOPTR010000537         2660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_30639_length_2660_cov_3.278311, whole genome shotgun sequence.
ACCESSION   DAOPTR010000537 DAOPTR010000000
VERSION     DAOPTR010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30639_length_2660_cov_3.278311"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(424..1290)
                     /gene="lpxA"
                     /locus_tag="PKI05_10370"
     CDS             complement(424..1290)
                     /gene="lpxA"
                     /locus_tag="PKI05_10370"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOA52650.1"
                     /translation="MGVRIHPTAVVNSFAALGTDVEIGPFCVVEADTIIGDGCRLESR
                     AVVKSGTTLGENNWVFEGAVLGGMPQHVHMPERPGRVIIGSGNVIRENVTIHRALDEE
                     DATLIGDNNLFMANVHIAHDCRVGNNTIFANNAMLAGHVTVGDRAYVSGAVAVHQFCR
                     IGAYAMVGGQAHIVKDVPPYVTVDGLSSYVVGLNQIGLRRAGFSTEAIAELKQAYRVI
                     YRSGLSWKDILEELRTKFSTGPASLFYQFLSTTTRGIIQERRTPQGVTLKLRRADDED
                     SDTAEWRVRITG"
     gene            1742..>2660
                     /locus_tag="PKI05_10375"
     CDS             1742..>2660
                     /locus_tag="PKI05_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52651.1"
                     /translation="MGSQGKPFWLLYAAYDRWSYRDTPAARFKFRWAHARFAGLARLF
                     VLFLVLLGIYHSAACAAQSFGEGATSAQHLVLAEVALSDPADATQVYAGKPYVWLGKY
                     IVSIQINGPLVDGLVLQLSAGAKNDRRGLAYEWIEQGKTIESGRTTYGGCDGFQDLAF
                     ELPARSGDSLEFRLSAASDKAAFFSHAALICHGGKTEGSLPKFTLRVNLMPITVRGPM
                     DGGFADRYPEMQAIWRDPLALSPDEVPEGSAEWAFRCAARNGRLANEMFFRASKYVQG
                     WLEQADPKTGLIPRNLTQSRDIWNPEDAAA"
BASE COUNT          550 a          729 c          786 g          595 t
ORIGIN      
        1 cggcgaaccc ggttttggat gccggggttc atgtctgggg tgccggccgt cacctcttcc
       61 tgctggagct ggacccgtca gaaaacggtg atgggcaaga acgtgtcgag tgagatcacg
      121 gccgggcgga cgagacagaa ctacctaaca acgttttggc tcggtctccg tcgccggcgc
      181 acaccggcgg gtcgcgattc cgcattgcgc cgagcgcatc cggggccctc ggcgtgagac
      241 ggcggtccag caagccgcgg gcggtccaag tgagcgaaag cggcgcacaa ggggcgcaca
      301 gtccggcaca gcatccgtgg gcggagaata gcaccgaact gcaagatacg aaatgaatta
      361 tgaccggata cggggttagg cttgatgttg cggacgaatg ccctgcgcga taccgtcgat
      421 gaatcaaccg gtgatccgca cacgccactc ggcggtgtcc gaatcttcgt cgtcagcccg
      481 gcggagtttg agggtcacgc cctgcggcgt ccgacgttct tgaataatac cccgggtggt
      541 cgttgacagg aactgataaa agaggcttgc cggaccggtg ctgaatttgg ttctcagctc
      601 ttcgagaatg tccttccaag acaggccgct gcggtagatc acccgatatg cctgttttag
      661 ctcggcgatg gcctctgttg agaaccccgc ccggcggagt ccaatctggt tcaagccgac
      721 gacgtaactg cttaatccat cgacagttac gtaaggtggt acatccttga cgatgtgggc
      781 ttggccgccc accatagcgt aggccccaat gcggcaaaac tgatggaccg ccacagcacc
      841 ggagacgtac gcccggtcgc ccaccgtcac atgcccggcc agcatggcgt tattggcgaa
      901 gatcgtgttg ttaccgacgc ggcagtcatg ggcgatgtgg acgttggcca tgaaaaggtt
      961 gttatccccg ataagggtgg catcctcttc gtccaatgct cggtggatcg ttacgttttc
     1021 tcgaatgacg tttcctgagc cgatgatgac tcggcctggt cgttcgggca tgtggacgtg
     1081 ttgcggcatt ccgccgagga cggccccttc aaacacccag ttgttttcgc caagggtcgt
     1141 cccgcttttg acgacggccc ggctttctaa acgacaacca tccccgatga tggtatcggc
     1201 ttcaacgaca caaaatggac cgatctccac atcggtccct agtgcggcaa aagagttcac
     1261 cacggcggtt gggtgaatgc gaacgcccat gatcgacctc catgtctcag aatagctttt
     1321 tgccgaaata gtcctcgacg cttgacttga cacgctagcc gcgcggggga aaaactgcac
     1381 aagccccgct tgggtgtgtc ctctactatc ttcacaagcc gacggcgcat gcgcgccgct
     1441 taacgtgtta aagtgtatcg gaagtgccga gaattgccaa tcacaattta cgggcttttc
     1501 caagacaaac gtgaggtgga gggtacccca ccggggtgtg tcgtcttttt gataatagca
     1561 gtgactatag agccaggtct tttggtgacc cgatgtaagt tccaaattgc tttgaggcaa
     1621 gcctcgtccg tcagtattcg gatcaactgt tttgacccca gatgcatgac ccggatcgat
     1681 gtgctacaac agacgccaaa tcttccggac agttaacaca aataccctac ggaggtgtgg
     1741 catgggttcc cagggcaagc cgttttggct gctttacgcg gcttacgatc gttggtcgta
     1801 tcgtgacacg cctgctgcgc ggtttaaatt ccgatgggca cacgccaggt tcgccggttt
     1861 ggcgaggttg ttcgttttat ttctcgtcct gttggggatc taccattcgg cggcatgcgc
     1921 tgcacaatcg tttggggaag gcgcaacctc ggcgcaacat ttagtgctgg cagaggtcgc
     1981 cctttccgat ccggctgacg caacccaagt ttacgcaggg aaaccctatg tttggctggg
     2041 aaaatacatc gtttccatcc agatcaacgg gcctttggtt gacggattgg tgctccaact
     2101 ctcagcaggg gccaagaatg atcggcgtgg cctggcttat gagtggattg agcaaggaaa
     2161 gaccatcgag tcaggacgga cgacttacgg gggctgtgac ggttttcaag acttggcctt
     2221 tgaacttccc gccagatccg gagattcgtt ggagtttcgt ctttcagcgg cctctgacaa
     2281 agcggccttc ttctcgcacg ccgctttaat ctgccatggg gggaagacgg agggttcact
     2341 tccaaaattc actcttcgcg taaacctgat gccgatcacg gttcgtggac caatggatgg
     2401 tggttttgct gatcggtatc cggaaatgca ggcgatttgg cgtgatccgc tagcactttc
     2461 tcccgacgag gtccccgaag gctctgccga atgggcgttt cgttgcgcag cgagaaacgg
     2521 gcggctggcc aacgagatgt tctttcgagc cagtaagtac gtgcagggct ggcttgagca
     2581 ggctgaccct aaaacgggtc taatccccag aaacctcaca caatcccgcg atatttggaa
     2641 tccggaggac gccgcggctg
//
LOCUS       DAOPTR010000538         2658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_30671_length_2658_cov_3.883596, whole genome shotgun sequence.
ACCESSION   DAOPTR010000538 DAOPTR010000000
VERSION     DAOPTR010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30671_length_2658_cov_3.883596"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PKI05_10380"
     CDS             <1..846
                     /locus_tag="PKI05_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOA52652.1"
                     /translation="WFIQVLQTEDLQQGAEWRYDVSRINELRRQDRFFRLFQPLIQLL
                     ARFNRAAFRDALPEIQRHINCAGLPRFWLAEEYLAKLELIALLLLPIYVYVAVRAVGA
                     GGILIAVVFAGLTVFLLRLRLADRAGRRLQAIKRRLPFLLDLLTLLMEAGSTFLNALA
                     QAVEEYRGHAIAEEFGRVLADIRMGKTRYEAFSAMRERLADDEITSIIGSILQGETLG
                     TPLASIFRTQADVLRIKRSQRAEMIAGEAGVNMLLPGILVMAAAVLILVGPFLMNYLY
                     FGMSL"
     gene            1243..2193
                     /locus_tag="PKI05_10385"
     CDS             1243..2193
                     /locus_tag="PKI05_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52653.1"
                     /translation="MTVGDTALDRTVGCPWCRHRFRADAVLAQGAKPTSPKGEVPLAT
                     PPTAPKGAEPAKKPARTGETPGPVGRVATPDGAAQPVSKEEARLSKPVSVGPGQPSPG
                     KTAGRDEGRGVLADKAGQASERAAGVAPGKPSPGESPAGGDTSLTMPRRRVARLILTE
                     TAEPKWTLAPDGSLPSLHLEERSDEERGKEKRRSSNPLLLILVLCSSLATSLILLFVD
                     TSPTTQGSPEEKARARQILAAEYYSHLNPNAPLAEYQRLLREAHWAHSRGDYKREKEL
                     YRQVLLLLYAERGRNTYTGLTGSRTRDQQLEELLRILLKD"
     gene            2283..>2658
                     /locus_tag="PKI05_10390"
     CDS             2283..>2658
                     /locus_tag="PKI05_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOA52654.1"
                     /translation="MGAAARYEIIGTIATGDFATVYRGRDRELGRDVAIKEIHPHFRR
                     DPKQLERYWREAQLLASLQHPHVITIYDVVKSRGWLILELMRTSLQKSIEKGPIDLDF
                     LREILIGSLEALDFLHRNGIVHG"
BASE COUNT          600 a          688 c          791 g          579 t
ORIGIN      
        1 tggtttattc aggtgcttca aactgaggat ttgcagcaag gagcggaatg gcgttacgat
       61 gtcagccgca tcaacgagct ccgccgtcag gaccggttct ttcggctttt ccagccgttg
      121 attcaactgc tcgcccgctt caatcgggcg gcctttcggg atgccttacc agagatccag
      181 cggcatatca attgtgcagg tttaccccgg ttctggctgg ccgaagaata tctggccaag
      241 ctggaattga tcgccttgct ccttttgccg atttatgttt acgtggccgt gcgtgcagtg
      301 ggggcaggtg gcattctgat tgcagtggtg tttgctggtt tgacggtatt ccttttacga
      361 ctccgcctgg cggatcgtgc agggaggcgg cttcaggcca tcaagcggcg tttgccattt
      421 cttctggatc tgctgacgct cttgatggaa gcgggatcca catttctcaa tgccctggca
      481 caagcggtcg aagaatatcg cggacatgcc attgccgaag agttcggtcg cgtgctggcc
      541 gacattcgca tgggcaaaac gcgctatgag gccttctcgg caatgagaga gcgcctggcc
      601 gatgacgaaa ttacgagtat tattgggtcg atcctacagg gtgaaacatt ggggacacct
      661 ctggcttcaa tctttcgcac ccaggctgac gtgttacgga ttaagcgatc ccagcgagca
      721 gaaatgattg ccggggaggc aggcgtcaat atgttgctgc cgggtatttt ggtcatggcc
      781 gccgccgtgt tgatcttggt aggcccgttc cttatgaact atctgtattt tggaatgtcg
      841 ttgtgagcgt tattttcatg gcatgtgagc caggggtgtg acgacttggt cagcgaaaat
      901 cagagcctat tccaaaaagt cgccctctcc ctccgggaga gggttggggt gagggcaagc
      961 gaacgtcgga atcaattgag aaaaccggca gcgattcgag ctaccctcac tcccgaccct
     1021 tctccggccg aacgggtgag gggagattat ggataggctc tcaggagatt tggttgactg
     1081 gcttttgtta aggcaatcgt agccgaatcc aacgatgact tgggcaagca atgccggatg
     1141 aaaagctgag aaggaattcc tatcgggtta acatcaaaca gagcacatcg gtcgaacctt
     1201 tcgatgccga ttaaaatcca ttgtccttta tgcggaacgg aaatgacggt cggagataca
     1261 gccctcgacc gaacggtggg ctgtccgtgg tgccgtcatc gctttcgagc ggacgctgtc
     1321 ttggctcagg gagctaaacc gacctcgcca aagggagagg ttcccctggc gactccccca
     1381 actgcaccta aaggtgccga gccagcgaag aagccagcga ggactgggga aacaccggga
     1441 ccggttggcc gggtggcgac tcctgacggg gccgcgcagc ccgtgagcaa agaagaggct
     1501 aggctgtcca aaccggtctc agtcgggcct ggacaaccaa gccccggaaa aacagccggg
     1561 cgggacgagg gacgcggtgt tcttgctgat aaggcaggtc aagcgtcaga acgagcggcc
     1621 ggcgtggcgc cggggaaacc cagccctgga gaatcacctg cggggggcga cacctcactg
     1681 acgatgccac ggcgaagagt agcccgactc atccttacag aaacggccga acccaagtgg
     1741 acgcttgccc cggacggcag tcttccgagc ttgcatctcg aggagcgcag cgacgaggag
     1801 cgagggaagg aaaagagacg atcgagtaat cctcttcttc tcattttggt gttgtgcagc
     1861 agtttagcga caagcttgat cctgcttttt gttgatacat caccgaccac gcagggaagt
     1921 cccgaagaaa aagccagggc ccgccaaatc cttgccgcag agtattattc tcacctcaat
     1981 cccaatgccc ccttggcgga atatcagcgt ctcttgcgag aggctcattg ggcacacagt
     2041 cggggtgact ataaacggga aaaagaactc taccgccaag tacttttgct actctacgca
     2101 gaacggggcc gaaataccta tactggattg acagggagtc gcacccggga tcagcaactt
     2161 gaggaattgc tgcggatcct tcttaaggat tgaatctcgg cgtcaggccg ctccctcggc
     2221 gggattagaa acgagaggcg atcaagaacg ttcgaaccac gggaggcaag tcgaggttgc
     2281 ccatgggcgc cgccgcacga tatgaaatca tcggaacaat cgccacaggt gatttcgcca
     2341 cggtttaccg ggggcgggat cgggaattgg ggcgggacgt tgccatcaag gagattcatc
     2401 cccatttccg gcgtgatccc aaacaactcg agcggtattg gcgtgaagcg cagctcctgg
     2461 cgtccttgca gcatccccat gtcattacga tttatgatgt cgtcaaatcg cggggttggc
     2521 tgatcctgga gttaatgcgg acaagtcttc aaaaaagcat tgaaaaaggt ccgattgatc
     2581 tggactttct ccgagaaatc ctcattggca gcttggaagc ccttgatttc ctccacagaa
     2641 acgggattgt ccacggtg
//
LOCUS       DAOPTR010000539         2657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_30684_length_2657_cov_3.481553, whole genome shotgun sequence.
ACCESSION   DAOPTR010000539 DAOPTR010000000
VERSION     DAOPTR010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30684_length_2657_cov_3.481553"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PKI05_10395"
     CDS             complement(<1..860)
                     /locus_tag="PKI05_10395"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOA52655.1"
                     /translation="MWPALPTWMCWTLLVVGGAIFGAAANWLADALAWQPRRISPFFP
                     TESGSRRRPIEYLPILGWLFRTGDSERHGRFFWIRPLAVELVCAVAIPLLYWWEVIQA
                     GLVPSGVPRPADGVLHAMFARHLLLIFFMLVASLIDWDEKLIPDSVTIPGTLLALILA
                     ASLSTSQLPHGIVHRAVFLGDDVMEFGKPEYSYLTFSAPYAWPKAFGGQPHGHGLSLG
                     LACWWLWCFALMPRRWYRRKGFWKSLKMMCARLYRSRVTGGLVVMGLIGSAGILFTWI
                     VGGSHWQALLS"
     gene            complement(860..1402)
                     /locus_tag="PKI05_10400"
     CDS             complement(860..1402)
                     /locus_tag="PKI05_10400"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOA52656.1"
                     /translation="MNIVLIGYRATGKSTVARLLAERLGWQWVDLDVEIERQAGKSIA
                     RIFAEDGEPVFRDWESRVVAEFCGRSGWVIAAGGGAPLRAENRKVMRANGRVVWLTAS
                     PETIHRRMTTDPTTGARRPNLTSQGGIAEIIELLSKREPIYRETADWVVDTEGKSPDA
                     VVQEILEHLRPVMDQVGVNP"
     gene            complement(1756..>2657)
                     /gene="aroE"
                     /locus_tag="PKI05_10405"
     CDS             complement(1756..>2657)
                     /gene="aroE"
                     /locus_tag="PKI05_10405"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOA52657.1"
                     /translation="PGQLSFEEMTQVYRYEDIRHDTEVFAVIADPVGHSLSPIIHNAA
                     FQKFGMNKVYIPIRVPREDLYEFIDQAPQWGIKGLSVTIPHKEAILAKLTKADSIVRG
                     IGAANTVIFEGKERLGFNTDCRAAIEAIEVALGTPVGGRSPIHGKQALVLGAGGAGKA
                     VAFGLIRAGATVTLTDGDAEKARLLAEKLDCAYVPWDDRHSVACDILANCTPVGMHPN
                     VDESPFDAKYLRSHMLVFDAVYNPENTLLIKDARARNCKVVTGVEMFVRQACMQFKLF
                     TGRDGPADLMREVLKRATSAAKW"
BASE COUNT          603 a          816 c          712 g          526 t
ORIGIN      
        1 gacaaaagag cctgccaatg cgatccaccg acaatccagg tgaaaagaat ccctgccgag
       61 ccaatcagtc ccatcaccac caggccgccc gtcacacgcg accggtagag ccgcgcacac
      121 atcattttga ggctcttcca gaaccctttt cgccggtacc aacgtcgggg catgagggca
      181 aaacaccata gccaccagca ggccaaacct aacgaaaggc catgtccatg tggctggcca
      241 ccgaaggcct tcggccatgc atagggcgca ctaaacgtaa ggtagctata ttcgggtttg
      301 ccaaattcca tgacgtcatc accgaggaaa accgcccggt gcacaattcc gtggggaagc
      361 tgtgacgtcg aaaggctagc cgccagaatt aaggcgagca aggtccccgg gatggttacg
      421 gaatccggaa tcagtttttc gtcccaatca atgagtgagg ccacaagcat aaagaagatc
      481 agcaaaaggt gccttgcaaa catggcatgc aaaacaccat cggcagggcg tggcacaccc
      541 gaggggacca atcccgcttg aatcacttcc caccaataca aaaggggtat ggcaacggcg
      601 cagacgagct ccacggccag cgggcgaatc caaaaaaagc gaccatggcg ctcactatcc
      661 cccgtacgga aaagccagcc caggatcggc aaatactcga tcggtctgcg gcgacttccg
      721 gactccgtgg ggaaaaacgg cgaaatacgg cggggttgcc aagccaaggc atcggcaagc
      781 cagttggcgg cggccccaaa gatggcacct ccaactacca gaagggtcca gcacatccag
      841 gttggcaagg caggccacat cacgggttga ctcccacctg atccatgacg ggtcggaggt
      901 gctccagaat ttcctggact acggcatccg gtgattttcc ttcggtatcc acgacccagt
      961 cggccgtctc ccggtagatg ggctcgcgct tgctgaggag ctcaatgatt tctgcgatcc
     1021 ctccctggct tgtgagatta gggcgacggg caccggttgt tggatcggtg gtcattcggc
     1081 gatgaatcgt ttcgggtgag gccgtcagcc acaccacccg cccgttggcc cgcatcactt
     1141 tgcgattctc tgcgcgcagc ggtgcaccgc caccggcagc gatgacccac ccagaccgtc
     1201 cgcagaattc cgccaccacg cggctttccc aatctcgaaa gacaggttcg ccatcctcgg
     1261 caaagattcg ggcgatagac ttaccggctt gccgttcaat ctctacatcc aaatccaccc
     1321 actgccaacc caatcgttcg gcaagaagcc gggcaaccgt acttttcccc gtggcgcgat
     1381 acccgatgag cacaatgttc ataacttggg caaagatagg aaagccgatt catcgtgtgt
     1441 caaaatctca ttttccgctt gtctccatca tactgatcgg acgagctttg cgaaagattt
     1501 gtttactgcg gcttgccgcc tgacaacacg ctcttgccag ccatggcttg aaagcctatc
     1561 tgataatctc ccctcgcccg ttcggcagga gaggggtcgg gttgagagta gctcgaatcg
     1621 ctgccggctt tcttaatcga ttcgacgttc gcttgccctc accctaaccc tctcccgaag
     1681 ggagagggcg actttttggg ataggctctt gagagcctac gatcggttgc ggcacgtgtt
     1741 cctacggagg ccgcgtcacc attttgcggc ggaggtggcg cgtttgagga cttcccgcat
     1801 caaatcggcc ggaccatccc gcccggtaaa caacttgaat tgcatgcacg cctgccgcac
     1861 aaacatctcg acgccggtga cgactttgca attccgcgcg cgggcatcct taattagcag
     1921 cgtgttttcg ggattataca ctgcgtcgaa gacaagcata tggctccgca aatacttggc
     1981 atcaaatggc gattcatcca cgttgggatg catcccgacc ggtgtgcaat tggccaggat
     2041 gtcacaagcc acagaatgtc gatcatccca cgggacatag gcacaatcga gtttctccgc
     2101 gagcaatcgg gccttttcgg catcgccgtc ggtcaatgtg acggtggctc cggcacggat
     2161 cagcccaaac gccaccgcct taccagcccc gcctgccccc aggaccaacg cttgcttgcc
     2221 gtggatgggg cttcgccctc caaccggcgt tcccaaggct acctcgatcg cctcaatggc
     2281 ggcacggcaa tcggtattga accccaatcg ttccttgccc tcgaagataa cggtattggc
     2341 agccccgatg ccccggacga tgctgtcggc cttcgtcagc ttagcgagaa tggcttcctt
     2401 gtggggaatc gtcacgctca accccttaat cccccattgc ggcgcctgat cgatgaactc
     2461 ataaaggtcc tcccgcggca cacgaatggg gatatagacc ttgttcatgc cgaacttctg
     2521 gaaggccgca ttgtggataa tcgggctgag gctatggccg acaggatcgg caatcacggc
     2581 gaagacttcc gtatcgtgcc gaatgtcttc gtagcgatag acctgggtca tctcctcaaa
     2641 gctgagttga cccgggg
//
LOCUS       DAOPTR010000540         2655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_30726_length_2655_cov_3.632692, whole genome shotgun sequence.
ACCESSION   DAOPTR010000540 DAOPTR010000000
VERSION     DAOPTR010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30726_length_2655_cov_3.632692"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PKI05_10410"
     CDS             <1..338
                     /locus_tag="PKI05_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOA52658.1"
                     /translation="NGDGRFIYPPLSASVPGKTSEPVIAKPVSSIRWEMLREGIEDYE
                     MLWLLRGLLRKKGDRLPAERRKELEDLLAVPPTITTNMTTFTKSPKPIYEHRSKVAEA
                     IEKLSAAEP"
     gene            complement(442..2139)
                     /locus_tag="PKI05_10415"
     CDS             complement(442..2139)
                     /locus_tag="PKI05_10415"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOA52659.1"
                     /translation="MTEGLCRHAASIESKTAPSDPDATTVSETTVQVRENLTGGDTQV
                     SLAHTSACPSGPNEKQTAGATSVRDQAASRDPNLTLIASSFDWQTPPFVQQPTGVRQT
                     NFWLDLPVLTAPNEEDFRAQEATPPSPESTDVSHATFLTATAPDRAGGASHPTNQPLQ
                     AIPDPALEGRSTHATNNRRICKDDTCWLSDFLYCDPCRPKDPWKLPTPQFLACRGWEF
                     GGWVSGGISVVANNPADNYNGVVTFNDRDREFQMNQLNFYLEKVTQTNGCGWDWGGRV
                     DVLYGTDARFTQAIDGLESDWNQTERFYQVALPQFYLDVAYNDWLLRVGHFYSLVGYE
                     AVPVTQNFFYSHSYSHQYGQPFTHTGLLLSRKLNQWVFTAGLQRGNDQFDDTDGRDAV
                     GFLGGIRWTSYDERFAVAFGISADEQGIDRPITIYSLAGTWNVTSRLQYVLEHTYGES
                     EDPQIGKSQWYGLTNYLYYKINPCWQTGLRVEWFRDEDGVRVAGVGDGNRAVGPYPGD
                     FYEISLGLNWSPHPNLRVRPEVRWDWFDERRPVALYPYDAGDRDDQFLLGCDFIVTF"
     gene            complement(2461..>2655)
                     /locus_tag="PKI05_10420"
     CDS             complement(2461..>2655)
                     /locus_tag="PKI05_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52660.1"
                     /translation="ALTPTLSQRERELWDSLLGRNLSLPKLLVLANSLLAPRTIRILD
                     TTVTMLVRRTTLGLKPPHTM"
BASE COUNT          583 a          706 c          778 g          588 t
ORIGIN      
        1 gaaatggaga tggtcggttc atctatccgc ctttgagtgc ctctgtgccc ggcaagacat
       61 ccgaaccggt aatcgccaag ccggtgtcca gcatccgctg ggagatgctt cgtgagggga
      121 ttgaggatta tgagatgctt tggttgctcc gcggtttact gcggaagaaa ggcgatcgcc
      181 ttcctgcgga acggcgtaaa gagctggaag atctgcttgc cgtcccgccc accatcacta
      241 ctaacatgac gacgttcact aagtcgccca aacccattta tgagcatcga agtaaggtgg
      301 ccgaagcgat cgagaaattg tcggccgcag agccatgagg tcaaagcatt tggagcccag
      361 agggccaaaa aggaatacgg cgctgaagtt gtgaaactcc agcgccgtat tcgtttattg
      421 atacgaacga ctaacttctg gctagaacgt cacgataaaa tcgcaaccca agaggaactg
      481 gtcgtcgcgg tcgccggcgt cataggggta gagggccaca ggccgccgtt cgtcaaacca
      541 atcccaacgg acctcggggc ggacccgcag gttggggtgc ggtgaccagt tcagccccag
      601 gctgatttcg tagaagtctc ccggataagg accaaccgct cggttgccgt ccccaactcc
      661 ggccacacgc acgccgtcct catcccggaa ccactccacc cgcagaccgg tctgccagca
      721 cggattgatc ttgtaataca agtagttggt caagccatac cattggctct tgccgatctg
      781 aggatcctcc gactcgccgt aagtgtgttc caggacatat tgcaaccgag acgtgacatt
      841 ccaggtcccc gccaggctgt agatggtgat gggtcggtca atcccctgct cgtcggcaga
      901 gatcccaaag gccacggcaa aacgctcgtc gtagctcgtc cagcgaatgc cgcccagaaa
      961 gccaacggca tcccgaccgt ctgtgtcatc aaactgatcg ttccctcgtt gcagtcctgc
     1021 cgtgaagacc cactgattta gcttgcggct gagtaacaag cccgtatggg taaacggttg
     1081 accatactgg tgcgagtaac tgtgggaata aaagaagttc tgggtaaccg ggacggcttc
     1141 ataaccgact agggagtaga agtgacctac ccgcaaaagc cagtcattgt aggccacgtc
     1201 gaggtaaaac tgtggcaagg ccacctgata gaagcgctcg gtctgattcc agtccgattc
     1261 cagaccatcg atggcctggg tgaagcgggc atccgtccca tagaggacat cgacacggcc
     1321 gccccaatcc cagccacagc cgttggtttg cgtgaccttt tccaaataga aattgagctg
     1381 attcatctgg aattcgcggt cacgatcgtt gaaagtcacc acgccgttgt agttgtcagc
     1441 cggattgttg gccaccacgc tgatgccacc gctgacccag cctccgaact cccatccgcg
     1501 gcaggccaag aattggggcg tgggcaactt ccaggggtcc ttcggccgac agggatcaca
     1561 gtagaggaag tcggaaagcc agcatgtgtc atctttgcag atacgccgat tgttggttgc
     1621 gtgtgttgat cggccttcga gagccggatc gggaatggct tggagcggct gattggtcgg
     1681 gtggcttgcc ccacctgcgc ggtctggggc tgttgccgta agaaaagtgg cgtgtgaaac
     1741 atcagttgac tcaggactgg gaggagtagc ctcctgtgcc cggaagtctt cttcatttgg
     1801 tgcggttagc accggtaagt ccaaccaaaa gttggtttgg cgaactccag tgggctgttg
     1861 cacgaatggc ggtgtttgcc aatcgaacga actggcgatc aatgttagat tagggtctcg
     1921 gctcgcggcc tggtcccgaa ccgacgtcgc ccctgcggtc tgcttttcgt tcgggccgga
     1981 tgggcatgcg ctggtatggg caagcgaaac ctgcgtgtca cctcccgtta agttttctcg
     2041 gacttgaacc gtggtttctg agaccgtggt tgcatcagga tcgcttgggg cggttttcga
     2101 ctctatggat gctgcatgcc ggcagagacc ttccgtcacg ggagagggcc cctccttggg
     2161 ggcttgtatt gttattatgg tcgctgatcg atatgcccgc atcagaagga taattcccag
     2221 catccccgtt gcaacgaaaa gtgcgtacct gcggatcttt ccgaccatcg cttgcctccg
     2281 gccttaacca gactccatct ttcacgcggg ccctgccttt tttgcctgtg ttgaagggtc
     2341 aagatgcgcg ggaaagcaca gggtaaccgg tatgttgtgc gggatgtttt acaaatgact
     2401 ggaatgaaga acaaaacggt tcttccgtaa aatcgtatcg ggaaagatga atacaacggt
     2461 tcacatcgta tggggcggtt tgagaccgag ggtcgttctt cggacaagca tcgtaacggt
     2521 tgtatcaagt atgcggattg tgcggggtgc gagcaggctg ttcgctaaaa cgagtagctt
     2581 gggaagacta agatttcggc ctaaaaggct atcccaaagt tccctctccc tctgggagag
     2641 ggttggggtg agggc
//
LOCUS       DAOPTR010000541         2655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_30732_length_2655_cov_2.797692, whole genome shotgun sequence.
ACCESSION   DAOPTR010000541 DAOPTR010000000
VERSION     DAOPTR010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30732_length_2655_cov_2.797692"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /gene="ruvX"
                     /locus_tag="PKI05_10425"
     CDS             complement(<1..209)
                     /gene="ruvX"
                     /locus_tag="PKI05_10425"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOA52661.1"
                     /translation="MARSFEDEHPQLPSTPEIPAKGRIAGIDFGKTRIGIALSDPGRV
                     LATPWAVYQRRGPQQDAEYFRKLVK"
     gene            complement(286..1068)
                     /gene="nagB"
                     /locus_tag="PKI05_10430"
     CDS             complement(286..1068)
                     /gene="nagB"
                     /locus_tag="PKI05_10430"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOA52662.1"
                     /translation="MEVIIKKSYEELCQAAAQAVAQVLNSKPNAVLGLATGNTLVGLY
                     RELVRMFREGEIDFSQVVTFNLDEYVGLRKEHPQSQHYFMHEHFFKYVNIAPQNIYMP
                     SGTSQNYQAFCRWYEQRIKECGGIDLQILGIGTDGHIAFNEPGSSLGSRTRIKTLSRR
                     RLEEIKDLFDEGEELPIYAITMGVGTILEAKRIILLAHGYAKADAVAAAIEGPVTSMV
                     TASALQLHRDAIAYLDDEAASKLKLYEYYVWVQEKRPAAPGM"
     gene            1364..2152
                     /locus_tag="PKI05_10435"
     CDS             1364..2152
                     /locus_tag="PKI05_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HOA52663.1"
                     /translation="MSNSTISTQRLALVTGGSRGIGAAICTKLGELGFHVFVNYVHNE
                     EAANKVAQDICEKGGTATVLPGDVGSESDRRRMLSVIREYQRLDVLVNNAGITSVGRR
                     DILEATEESCEKVFNTNLKGPFFLTQAIANEMIRWIREGRQPQGYIINVSSISAFAVS
                     TNRADYCLTKAALAMMTQLFAQRLADEKIQVFEVCPGVIASDMTAPVKEKYDRLIAEG
                     AWPIRRWGEPEDVALAVAALVQGYFPFSTGNRFLVDGGFHIRTL"
     gene            complement(2165..>2655)
                     /locus_tag="PKI05_10440"
     CDS             complement(2165..>2655)
                     /locus_tag="PKI05_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52664.1"
                     /translation="HRYVGVLMQDCAYMVAVPFFCPDVPPLKRNPVFLFSYDSFQRPN
                     PFRADIVVAIDEVIDQKLDALVVIESQFVEGGALGSAEAAPKTEEERQKKREQVRESF
                     RQRFARIADQYRDRLIELYGPEKGRAVKYAEAFEICEYGRQPSAQEIRQLFPFIGQSD
                     SK"
BASE COUNT          600 a          727 c          705 g          623 t
ORIGIN      
        1 tgcttaacca acttgcgaaa atactccgcg tcttgctgcg ggccacgtcg ctgatagacc
       61 gcccaaggtg tggctagcac ccgaccaggg tcgcttaaag cgatcccaat acgggtctta
      121 ccgaagtcga tacccgcgat gcgccctttc gcaggaatct ccggcgtgct cggtaactgg
      181 ggatgctcat cctcaaatga acgcgccatt gttgattaat tcttttggca cttggcactg
      241 tcatcgcgct gtcagatggg cccaggccct gaagaattcg cgccctcaca tgcctggggc
      301 agccggtcgt ttttcctgga cccaaacgta gtactcgtac agcttgagct tggaggcagc
      361 ttcatcgtcc aggtacgcaa tggcatcgcg gtgtaactga agggcactcg cggtcaccat
      421 gcttgttacc ggtccctcga tggctgcggc caccgcatca gccttggcgt aaccgtgagc
      481 cagcaagatg atacgcttcg cctccaagat tgttcccaca cccatggtga tggcgtagat
      541 gggaagctct tcgccttcgt cgaataggtc cttgatctct tccagccgac gccgcgacag
      601 ggtcttgatc cgcgtccgtg accccaatga actcccaggc tcattaaagg cgatatgacc
      661 atcggtgccg atacccaaga tttggaggtc aatcccaccg cactctttga ttcgttgttc
      721 gtaccaccgg caaaacgcct ggtagttctg cgatgtgccc gatggcatat agatattctg
      781 cggtgcaata ttgacgtatt tgaaaaagtg ctcatgcatg aagtaatgct gactctgagg
      841 atgttccttc cgcaggccaa cgtattcatc aaggttaaat gtgaccacct gggaaaaatc
      901 tatttcgccc tcgcgaaaca tgcgcaccaa ttcacggtac agccccacaa gcgtgttgcc
      961 cgtagccaat cccagcacgg cgttgggctt cgaattaagg acctgggcaa ccgcctgggc
     1021 cgcggcctga cataactcct cgtaactctt cttgatgatg acttccatcg tttccctctg
     1081 cgaggttcgc cacctgtttt tgtacgtcaa gacgctacgc gatatagacg tccgtaaccg
     1141 gtgcagcgac aggaaatgga ccggaacgca atgaccctct tgaaattacc gttaagaagg
     1201 tcacactcaa taacttgagt gtcggcctaa tggggaccgc tgtcaagttc tgccaatggc
     1261 ctgggccacg gtgggacaaa acagctcggg gcacgaaaac cgtgccttag gaatttggtt
     1321 gatgtgcgtt agcaatcccg tcttgaggga gtatgagatg accatgagca actccacaat
     1381 ctctacccaa cgcttggccc ttgtcacggg aggatcacgc ggcattggtg cggcgatctg
     1441 cacgaagttg ggggaattgg gcttccatgt ctttgtgaat tacgtgcaca atgaggaggc
     1501 ggcaaataag gtcgctcagg atatctgcga gaaaggcggt acagcgacag tcctgcctgg
     1561 ggatgttggc tccgaatcag atcgccgtcg gatgttgagt gtcatccgtg aatatcaacg
     1621 gctcgatgtt ttggtgaaca acgcggggat cacctcggta ggacggcgtg atatactcga
     1681 agcgactgaa gaaagttgcg aaaaggtttt caatacgaac ctcaagggtc cttttttcct
     1741 cacccaagcc attgccaacg aaatgatccg ttggattcgt gagggccgac agccacaggg
     1801 ttatatcatc aatgtgtctt caatttctgc ctttgctgta tccaccaatc gggcggacta
     1861 ttgtttgaca aaagcggcat tggcgatgat gacgcaactc ttcgcccagc ggcttgcgga
     1921 cgaaaaaatc caggtctttg aagtgtgccc cggtgtcatc gccagcgata tgacggcccc
     1981 tgtcaaagaa aaatacgacc ggctcatcgc cgaaggggcc tggcccattc gcagatgggg
     2041 agaaccggag gacgtggccc tagcggttgc cgctttagtc cagggttatt tccccttcag
     2101 cacgggcaac cggtttcttg tggatggggg ctttcacatc cggacgttgt gattgccgcc
     2161 aacattattt ggaatcggac tggccgataa atgggaataa ttggcggatc tcttgcgcac
     2221 tgggctgccg cccatattca caaatctcaa aggcctcggc atactttacg gcccgtcctt
     2281 tttcaggtcc atacagttca atgagtctat cccgatactg atcggcaatt ctggcaaacc
     2341 gctgccggaa ggactcgcgg acttgttccc gctttttctg ccgttcctcc tccgtcttgg
     2401 gcgccgcttc ggctgatccc agggcacctc cttcaacgaa ctgggattca ataaccacca
     2461 aggcatcgag cttttgatcg atcacctcgt caatcgctac cacaatgtcg gcacggaatg
     2521 gattgggacg ctgaaaactg tcgtacgaga acaaaaacac agggtttctt ttcagtggtg
     2581 ggacatctgg acagaagaac ggcacggcca ccatgtaggc acagtcttgc atcaagacac
     2641 ccacgtaacg gtggt
//
LOCUS       DAOPTR010000542         2650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_30791_length_2650_cov_3.562235, whole genome shotgun sequence.
ACCESSION   DAOPTR010000542 DAOPTR010000000
VERSION     DAOPTR010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30791_length_2650_cov_3.562235"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..2226)
                     /locus_tag="PKI05_10445"
     CDS             complement(160..2226)
                     /locus_tag="PKI05_10445"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOA52665.1"
                     /translation="MNEPQQLGPYRIVRRLGRGGMGTVYEAVHVVTNEPAAVKTLSES
                     LIEEPDFRARFELEIETLRKLNHPNIVRLIGFGEEQGILFYVMELVHGPSLDQELRAG
                     RKFHWQEVAEIGVQICRALRHAHDRGIIHRDLKPANLLRAENGTIKLSDFGIARLFGA
                     PSHTGVGSILGTIEYMAPEQAESLPIGPRADLYSLGAVLYALLVGRPPFKVTSFAEIL
                     HLHRTSQPAPVRSFVPDCPEELDRIIRELLAKDPDKRIANALLAGRRLQAMLHALTKH
                     KVAGSEDVLHATPGHLIDFGVEQGDRRSVPVDGEAPTAALPSSPEPPRPSLGETITAA
                     GSPSESPENQAAAFPVGTPSMWKPTRETDVFRYLEKESPVPDHPEDGLGSTATAEPKE
                     QKGRTEQPSPPVTSSATQTTRKFVEVPPEELDKIEPSPSERQPIFSLQTGILLAALVV
                     LAITIWYFLQPPTADQLYNRVMAKVGDGTDSEALLSAAPDIEAFLTRYPEDARAPSIR
                     EYQQEIDLLRLERRFELQSRQKASAHSSIPIERDYLQALRYAQLDPELGIEKLEALLA
                     LYDYAPTEKLPADKSRETAQGKSRRPVGHTAECLELARRRLEQLKKQLAPAITNQRDL
                     IAGRLEEANQLEATDPEKADAIRRAIIVLYGDKGWAKPYIEQAEAALQRRSQKSLPSQ
                     ISTTSP"
BASE COUNT          544 a          704 c          796 g          606 t
ORIGIN      
        1 ctgaccgttg tgggcattca tcttttctac tggaccaata tgcggatgcg ggcgccgtta
       61 atccccatgc ttgctctttt ggcaggggaa gggtatcact acctcacgtc tcgccgattg
      121 gaaaccgaac aaacctaacg gtctcgctta ttagggcgat tacggggaag tcgtcgatat
      181 ctgggaagga agggacttct gcgatctcct ttggagggca gcctcggctt gttcgatgta
      241 cggctttgcc cagcccttgt caccgtagag gacgattatc gcgcggcgga tggcgtcggc
      301 cttctctgga tcggtggctt cgagttgatt ggcttcctct aaccgtccgg cgatcaaatc
      361 gcgctggttc gtgatggccg gtgccagttg ttttttcagt tgttccagac ggcggcgggc
      421 cagttcaagg cattcggccg tatggcctac agggcgacga gatttgcctt gagctgtttc
      481 gcgcgacttg tcggccggga gcttttctgt aggtgcataa tcgtaaaggg caaggagggc
      541 ctcgagcttt tcaattccaa gttcggggtc gagttgcgcg taacggaggg cttgaaggta
      601 gtctcgttcg ataggaatcg atgaatgagc cgaggccttt tggcgactct gcaattcaaa
      661 ccgccgctcc agacggagaa ggtcgatctc ttgttgatac tcacggatag atggcgctcg
      721 ggcgtcctcc ggataccggg ttaggaaggc ctcgatatcg ggcgcagcgc tgaggagggc
      781 ctcactatcg gtgccatcac ccaccttagc catcacccga ttgtaaagct ggtcagcggt
      841 cggtggttgc agaaaatacc aaatggttat tgccagcacg acaagagcag ccagcaggat
      901 tcccgtttgg agggagaaga ttggttgacg ctcactgggg gaaggctcaa ttttatcgag
      961 ctcttcgggg ggaacttcga caaattttcg ggtggtttgg gttgcagaac tggttaccgg
     1021 cggggacggc tgttctgttc gtcccttttg ttccttgggt tcagccgtgg cagtggaacc
     1081 taaaccatct tctggatggt ctggtactgg cgattctttt tccaagtacc gaaagacatc
     1141 ggtctcacgc gtcggcttcc acatcgaggg cgttcccacg ggaaaggcag ctgcctgatt
     1201 ttccggggat tcactcggtg aacctgccgc cgtgatcgtt tctcccagcg agggcctcgg
     1261 tggctcgggc gacgaaggaa gggcagccgt cggtgcctca ccatcgacgg gcacactgcg
     1321 gcgatctcct tgctcgacac cgaaatcgat gaggtggccg ggagttgcgt gcaagacatc
     1381 ttcggagcca gccactttgt gcttggtcag ggcgtgcagc atggcctgca atcgccgacc
     1441 cgccaaaagg gcgttggcaa tcctcttgtc ggggtctttg gcaagcagtt cgcgaatgat
     1501 gcggtccaat tcttcaggac aatcgggcac aaagctccgc actggggccg gctgcgatgt
     1561 ccgatggaga tggaggatct cggcaaacga ggtaaccttg aacggcgggc gcccaacaag
     1621 caaggcgtaa agaacggcgc ccaaactgta gaggtctgcc ctcgggccga tcggcaagga
     1681 ttctgcttgt tctggggcca tatactcgat tgtgccaagg atgcttccta cgccggtgtg
     1741 gctgggtgca ccgaaaagcc gagcgatccc gaaatcggag agtttgatgg tcccgttttc
     1801 cgcccgcaac agattggctg gtttcagatc ccggtggatg atcccgcgat cgtgggcgtg
     1861 tctcaaagca cggcaaattt ggacaccgat ttcggcgact tcctgccaat gaaacttccg
     1921 cccggcacgc agttcctggt caaggcttgg cccgtgcacg agctccatga cataaaagag
     1981 aattccttgt tcttcaccaa agccaatgag gcggacgatg ttgggatgat tcaacttccg
     2041 caacgtctcg atttccaatt cgaaacgggc acgaaaatcc ggttcttcga tgagggattc
     2101 ggataaagtc ttgaccgccg caggttcgtt ggtgacgacg tgtacggcct catagacagt
     2161 tcccattccc ccgcggccga ggcgccggac aatccgatag ggcccgagtt gttgaggctc
     2221 gttcatggca ggttcacttc accctgcaag catatttcca aggcaataag atcgagagcc
     2281 cctccgtatt tagtgtaacg gcttctccag aaaaggccag aaaaaagaaa gggacgcatg
     2341 catctgcgtc cctaagctct cgaaaaaccc gattttgacc ttacaagcct atcccaaagt
     2401 tcgctctccc tctgggagag ggttggggtg agggcaagcg accgtcggag tcgatcgaga
     2461 aagcggcagc gattggtgcc gccctcaccc ccggcccctc tcccgccgaa cgggagaggg
     2521 gaggttctgg gataggctcc taacttaccg cacaaggcgg caaaagttca tgtaatcact
     2581 ccagcacctt gatcttcaca ttgcggaagt agatcggtga atcgccgtgt ttgccttgaa
     2641 gaccgatgtg
//
LOCUS       DAOPTR010000543         2649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_30814_length_2649_cov_3.185042, whole genome shotgun sequence.
ACCESSION   DAOPTR010000543 DAOPTR010000000
VERSION     DAOPTR010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30814_length_2649_cov_3.185042"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="PKI05_10450"
     CDS             complement(<1..1121)
                     /locus_tag="PKI05_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52666.1"
                     /translation="MSIFVALVGVIFSVSQTGFAGSVAFLIPAARTTPLGQEAQAALA
                     WLQGNRDCRVIYLEGPEQFVNDRGEPVPLTEFQIVWCHQGDLPTIPADLQNPGTLEAL
                     RNFVSQGGRLFLSGAAARLVTDMGLETGVMRTASPGREGFQSALIPVVRDHPIFVGLP
                     ARPGAPIFFTSGGYPAFADFYRGGGPKTGRLLARANYADENPLVEYRLGKGRVIILGW
                     RIPNYALANNVHRRALDRLTANILDYLAHDAKWVDWEESETSAISMAKAEIPSTSELA
                     ALKAAIDDLITSFGDRYREGHLFQDRWEKLKTACDDLRRRNSVDEQAIQQVRTSFLEL
                     QQEALLANPLLDFDTLLYVERKEPKLGLPANWESHSSLPP"
     gene            complement(1468..1686)
                     /locus_tag="PKI05_10455"
     CDS             complement(1468..1686)
                     /locus_tag="PKI05_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52667.1"
                     /translation="MRKLIVAILGAFLLSSVVGCVVPIYSADPDRRVQQLIYTSEGLR
                     LMLDEWERAWMLDHPDHMTPYRTHGGII"
     gene            complement(1872..2168)
                     /locus_tag="PKI05_10460"
     CDS             complement(1872..2168)
                     /locus_tag="PKI05_10460"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOA52668.1"
                     /translation="MTKKDIVRRIAEELGVSQTLTREIVQRTFDAIIDTLTTEKRIEL
                     RNFGVFRIKTRRPRRAINPRTKESLQVPEKYVVRFKPGKELKKRLKAMSKKPRP"
BASE COUNT          610 a          718 c          688 g          633 t
ORIGIN      
        1 gggggaagac tcgagtggct ttcccagttg gctggtaagc ccaatttggg ttcctttctc
       61 tcgacataaa gcagtgtatc aaagtccaaa aggggattag cgagcaaagc ctcttgttga
      121 agctcaagaa acgacgttct gacttgctgg attgcttgtt catcaacaga attgcgacgc
      181 cgtaaatcat cacaagccgt tttcagtttt tcccacctgt cttgaaaaag atggccttcc
      241 cggtagcgat cgccaaatga ggtgatcaga tcgtcgatgg ccgcttttaa ggctgccaac
      301 tcgcttgtgc tgggaatttc cgccttcgcc atagatatcg ccgatgtctc ggactcttcc
      361 cagtccaccc attttgcatc gtgagccaga taatcgagaa tattggcggt gagtcgatct
      421 aacgctcggc gatggacatt attggccagg gcataattgg ggattcgcca gcccaggata
      481 atgacgcggc cttttccaag ccggtactcg accagagggt tctcgtcggc atagttcgca
      541 cgagccaaga gtcgccctgt ttttgggcct ccgcccctgt aaaaatcggc gaatgccgga
      601 tatcccccgg acgtgaaaaa gatgggcgca cccggccggg cagggagacc cacgaagata
      661 gggtgatctc ttaccacggg gataagtgca ctttgaaagc cctcgcgccc aggcgaagcc
      721 gttcgcataa cgccggtttc tagccccata tccgtaacca accgtgcggc cgctcccgag
      781 agaaataacc tcccaccttg gctgacgaaa ttgcggaggg cctccagcgt gccagggttc
      841 tggagatcag ccggtatcgt gggcaaatct ccttggtgac accaaacgat ctggaactcg
      901 gtcaatggta cgggttctcc tcgatcgttg acgaattgct ctggaccctc gagataaatc
      961 acacgacagt cgcggttacc ctggagccag gcgagagccg cttgggcttc ttgacccagc
     1021 ggcgttgtcc gtgcggcggg gataagaaat gccacagagc cggcgaatcc ggtttggcta
     1081 acagagaaga tgacacccac cagcgcgaca aaaattgaca cacatttaat catccgcaga
     1141 cctcacatgc catcgggaat cctgaagatc ggcgctactg cgcagcgact cgcaattatc
     1201 caacgctcag tcaaatccaa gagccaattt tattaaaaga ggaggccgaa agcgacagtt
     1261 gagcgaggaa acaatgacta gaaagagcat gaacgagtat aagtgtatcg ttggggacaa
     1321 aataaaatca gccctgccgg ttgacgaccg gcagggctgt gcgtgtcact gtaccttaca
     1381 ccaaacttgg gcagaggcac ggcactcaaa tggcttcctg ccttcaccca gttgatgctg
     1441 ttgcttttgt ggcaatcaga cagagtatca gatgatacca ccgtgtgttc ggtacggggt
     1501 catgtgatca ggatgatcca gcatccaggc gcgttcccat tcgtcaagca taagccgtag
     1561 tccctcggac gtgtagatca attgttggac acggcggtca gggtcggctg agtagatggg
     1621 aacaacacaa cccacgacac tgctgagcag aaacgcaccg aggattgcca caatcagttt
     1681 tcgcattttg ccactccgtc agcataagtt gacgagccaa ccttgctcgt catccgcgaa
     1741 cgttctctcc atgtaaccgg aaacacacgc cctgttgttt cgtgtatttc tgaggactcc
     1801 caggggcttc ggggcctcat tgatctcgcg gtaattcgaa tgtcccgccc attggaattc
     1861 gccgcggttg gttagggtcg cggcttcttg gacatggcct taagcctttt ctttagttcc
     1921 tttcccggct tgaatcgcac tacatacttc tctggtacct gaagcgattc tttagtcctg
     1981 gggttgattg cccgacgggg tcttcgtgtc ttgatccgga acacgccaaa attccggagc
     2041 tcaatccttt tctcggtggt caatgtgtcg ataatggcgt caaatgttct ctgaacaatt
     2101 tctcgcgtca gggtctgtga aacacccagt tcttcggcga tacgtcgtac aatgtccttt
     2161 ttggtcacgt gaattacctc cggcgaaccc gacgagtttt ctcgatttca actaccgatc
     2221 gttgtaaatc tagttcctga cgaaactaaa atcaattacc cgtcgcgccg tagggttctg
     2281 gttcgctgac cacaagaaac ctggctttct gtggccgatt ggggacaact cttaagtgac
     2341 gccaccctgt ctcatcgggg tgttccgtct tatttgaccc cgaccgcggt acaacacggt
     2401 cgtcacaggt ctcacatcca atatcggccg aaaaatccga aaactccaac ttcccttgtg
     2461 atttctccgc cacagagatg acaggatagg caaagtgcgc aaaatgccag tggggctgcc
     2521 tttttctatg cccgaggcaa ctaggctcga tcggtttcga aattggtttc agatgtcaat
     2581 catttgtttc cgccggtcga tctcctctag cgagtgcagt ggcccttggc cgcagcctgg
     2641 gcaggtccg
//
LOCUS       DAOPTR010000544         2649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_30815_length_2649_cov_3.114109, whole genome shotgun sequence.
ACCESSION   DAOPTR010000544 DAOPTR010000000
VERSION     DAOPTR010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30815_length_2649_cov_3.114109"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PKI05_10465"
     CDS             <1..745
                     /locus_tag="PKI05_10465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOA52669.1"
                     /translation="RVLDDQGSICWQVGNYVDNGEIIPLDIVLYPIFASLGLHLRNRI
                     VWHFGHGLHASKRFSGRYEVLLWFTKGDEYTFKLDAVRVPQKYPNKKYFKGPKKGELS
                     GNPLGKNPGDVWEIPNVKANHVEKTIHPCQFPVELIERLVLAMTDKGDWVLDPFIGVG
                     TTAIAALMHGRKAIGAEIMPEYVEIAKERIRLAERGELRIRPMERSVYDPDMPDASIP
                     PQVVRLGSARTELPLFEQIARYSAQEQEE"
     gene            742..1233
                     /locus_tag="PKI05_10470"
     CDS             742..1233
                     /locus_tag="PKI05_10470"
                     /inference="COORDINATES: protein motif:HMM:NF020757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HOA52670.1"
                     /translation="MRIVYEYSHLGGAEILKVHYKEHEDEIKEIIAGVKASKTKISKE
                     KTMQGRELYSPKDMNQQFREAFAARGYAELRDTYTITIPNSDVAIPGAFKQIDFVKGK
                     VLVEVQFGKYAFMFYDMAKFQCFLTRIRRTWASRLSPATPCISKCPPAYPSENNWYTI
                     LSA"
     gene            1799..>2649
                     /locus_tag="PKI05_10475"
     CDS             1799..>2649
                     /locus_tag="PKI05_10475"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOA52671.1"
                     /translation="MMIRDKRSEIVIFGVAMLVLLLPLAGLAETIPSPLPDPDGKPAD
                     MSQPVQVFILLGQSNMIGFGKVSGGEGSLEYAVKEKGLYPYLIDDAGNWTVRYDVRYV
                     RVMGSGTGPIKVFNNEWLTVSGRTIGPEIGIGHYLGEALAAPVLLLKSCIGNRSLGWD
                     LLPPGSKQYEYKGKIYAGYKESPMSWDKGTKPEPIGWYAGMQYDGDIARAKMVLEELD
                     KYYPGAKSYEIAGFFFWQGDKDRYNEAHAVHYEENLVRLIEQLRRDFNAPNAKFVCAT
                     LGQTEKGA"
BASE COUNT          657 a          644 c          739 g          609 t
ORIGIN      
        1 gcgagtttta gacgaccaag gaagtatttg ctggcaggtg ggtaactatg tcgataacgg
       61 cgaaattatc cctcttgaca tcgttctgta tccgatcttt gcctcgttag ggttgcattt
      121 gcgcaatcgt attgtgtggc actttggaca tggcttacac gcttcgaaac ggttttcggg
      181 ccgatacgaa gtccttctgt ggttcaccaa aggcgatgag tacaccttca agctggacgc
      241 ggtgcgcgtg ccgcagaagt atcccaacaa gaaatacttc aagggaccga agaagggtga
      301 acttagtggc aaccctttgg gtaaaaatcc gggggacgtt tgggaaatcc ccaacgtcaa
      361 agcaaaccac gtggaaaaaa ccatccatcc ttgccagttt ccggtcgaat tgatcgaacg
      421 tctggtgctg gcgatgaccg acaagggtga ctgggtgttg gatccgttca taggtgtggg
      481 aacaacagca atcgctgccc tgatgcatgg ccgaaaagcc attggtgccg agatcatgcc
      541 tgaatacgta gagattgcga aagagcgcat ccgcctggca gagagagggg aattgcgcat
      601 caggccgatg gaaaggtcgg tttatgaccc cgatatgcca gacgccagta tcccacccca
      661 agtcgtacgt ctggggtcag cacgaacgga gctcccttta ttcgagcaga ttgcccggta
      721 ctctgcgcag gagcaagaag aatgaggatc gtgtacgagt attcgcacct gggtggggcg
      781 gaaatcttga aagtgcatta taaagaacat gaagacgaga ttaaggaaat cattgccggt
      841 gtgaaggcaa gcaaaactaa gatcagcaaa gagaaaacga tgcaaggaag agaactgtac
      901 tctcctaagg atatgaatca gcagtttcgt gaggcattcg cagccagggg ttatgctgag
      961 ctgcgcgaca catacacgat taccataccc aatagcgatg tagccatccc cggtgcgttt
     1021 aagcagattg atttcgtaaa agggaaggtg ctggtggaag tgcagttcgg caagtatgca
     1081 tttatgtttt acgatatggc taagttccag tgctttttaa cgagaataag gcggacgtgg
     1141 gcgtcgagat tgtcccctgc cacgccctgc ataagcaaat gtccaccggc gtatccttcg
     1201 gagaacaact ggtatacgat attgagcgct tgaagcgaca tttccctgcc gtcccggtta
     1261 aggtgatttt gattgatgca gacactaatt gagacgcctc tcaataaacc ctcaatgttc
     1321 atttggccgc atgcgagcac atagtggggg tgcctaccac acgctgtccg gttgtcctcg
     1381 cagcctgcac agcctgccag gatgctgtcc gaacaaaacg tcacaattgt ttggccgttg
     1441 ctgcttttgt tttagtttcg ctatccgtgc atgccatgac ggaccatccc attcggggct
     1501 tctacgcgct ggtaacggct ttggggaggg ccatcagcgc cattgtcgcc ggacaaatag
     1561 ttgaaccaac ataaggcttg cggagaatgc ttatggtctc gacaattgtt ggtaccactc
     1621 tttgagcctg gacaattctc ggcaaatcgg ttggttgcga ggttgacaag ccagataccc
     1681 ctattcgaga ccatggattt tcaacccctg cggccatagt gctcctcgcg ggatcacaag
     1741 agtaagaacc ccgtaaagaa tcacgtgctt tggaaacggt tactgtcggg aggcctgaat
     1801 gatgattcga gacaagcgga gcgaaatcgt catctttggt gtggcaatgc tggtgctctt
     1861 gttgccattg gccggacttg ctgaaacgat cccctcgccc cttcctgatc ccgacggcaa
     1921 gcctgcagac atgtcccaac ctgtccaggt cttcatactc ttgggacaat ccaatatgat
     1981 cggattcggc aaggtaagtg ggggcgaagg ctcgcttgaa tacgcagtga aagaaaaagg
     2041 gctttatcct tatcttattg acgacgccgg caattggacc gtgcgttacg acgtccgcta
     2101 tgtccgcgtg atgggcagtg gcacaggccc cataaaggtc ttcaacaacg agtggttgac
     2161 cgtatcgggg cgaacgatcg gcccggaaat cggtatcgga cactacttgg gagaagccct
     2221 tgctgcccct gtgctcttgc tcaaaagttg cattggcaac cgctcgctgg gctgggactt
     2281 gcttccgcca ggttccaagc aatacgagta caaggggaag atttacgctg gctataaaga
     2341 atcgcctatg agttgggata agggaacaaa gcccgagcca atcggctggt atgccggcat
     2401 gcagtacgat ggtgacatcg ctcgggcaaa aatggtgctc gaagagcttg acaagtatta
     2461 ccccggcgcg aagagctacg aaattgcggg attctttttc tggcaggggg ataaggaccg
     2521 ctacaacgag gctcatgccg ttcactatga agagaacctc gttcgcctga tcgaacagct
     2581 tcgccgggat ttcaatgccc cgaacgccaa attcgtctgt gccacgcttg gtcagaccga
     2641 gaagggcgc
//
LOCUS       DAOPTR010000545         2646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_30862_length_2646_cov_2.510228, whole genome shotgun sequence.
ACCESSION   DAOPTR010000545 DAOPTR010000000
VERSION     DAOPTR010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30862_length_2646_cov_2.510228"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PKI05_10480"
     CDS             <1..526
                     /locus_tag="PKI05_10480"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOA52672.1"
                     /translation="GHFWLSTTPDVPGSKSWDSALPRMVTWVKLRDRKAPNLRPILWF
                     NTHFDHRGKQARQESARLLRKKLLTMGEDCSLIVTGDFNAAEGSRPYQLLFGDTEGQP
                     SPIVDSFRAAHPERGEEEGTFSGFRAENTKGARIDWIGCSRDWKVLEAGIDRTARQGR
                     TPSDHFPVFAILTR"
     gene            complement(765..>2646)
                     /locus_tag="PKI05_10485"
     CDS             complement(765..>2646)
                     /locus_tag="PKI05_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HOA52673.1"
                     /translation="AVMREQLQQLQRVAKEKEKNNEPLDDATRQLMERIQDRINDWQN
                     FPANRAIVNLAIVEVTVAPDAEPGPREIRLVTTQGVTNPLPFYVGQFPEYVRKPIKAC
                     QFPVLGNEQAAERRRPPEEKEVRVTIPCVVNGQVAPGFVNAYRFEARKGQRLLIKAYT
                     RDLIPYMADAVPGWFQSMLTLYDAQGQEVAFSDGFRFHPDPVIYYEVPQDGEYVLTIS
                     DALFRGREDFVYRISIAESPFITSVFPLGRKLGESITPEVKGWNMEGAELRLPPADAG
                     SGVYFVTAVKNGLVSNRWPFAVDTLPEIMEKEPNNKPAEAEKVSLPVIINGRIDQPGD
                     LDVFCVEGKAGQTLVAEVTARRLDSPLDSVLRITDASGKPIALNDDYEDPGMGQMTYH
                     ADSYIMIPLPADGAYYVYLGDTTRKGGPEYAYRLRLSEPRPDFALRVVPSAVAVRSAS
                     ANTVRVYAIRKDGFAGDIQVSLKDPPEGFANAPVVLRANQEMVGLTIKTSLKDSDGAI
                     PLRVEGRAKVGDQEIVHEAVAAEDRMQAFLWRHLLPVADLRVRVYDPNYQPQPARTPP
                     EVPPEMVEAARKAAQEAGRAPFSKAQVAGRVRMLNLLYQNGFLTDDFYLVKMAECAAA
                     QD"
BASE COUNT          569 a          762 c          724 g          591 t
ORIGIN      
        1 tggtcacttc tggctcagca caacaccgga tgttccaggg agcaagagtt gggatagcgc
       61 cttgccccgg atggtcacct gggtcaaact gcgggaccgt aaagcgccta acctccggcc
      121 cattctttgg ttcaacacac actttgatca ccggggtaaa caggctcggc aagagtcggc
      181 ccggctcctc cgcaaaaagc tgctcacgat gggcgaagat tgctccctca ttgtgaccgg
      241 tgatttcaat gccgcggaag gaagtcggcc ctatcaattg ctattcggag acaccgaggg
      301 tcaaccctct ccgattgtcg atagctttcg ggcggctcat cctgagcggg gcgaagaaga
      361 aggtacattt tcgggctttc gggccgaaaa tactaagggg gcacgcatcg attggattgg
      421 gtgttcgcgg gattggaaag tccttgaggc tggcatcgat cggacggccc gccagggacg
      481 aaccccctcg gatcactttc cggtcttcgc catcctgacc cgttgacctg gtaatccagc
      541 ggctcggggc cggtgtctca ccactaccga aaggatcgcc gagctgcggt ttttcgactc
      601 gcaagactcc ctttggccgg aagaaaaccc gtccgggttc gattcctgac aaaactccac
      661 ccgtgagggt atgcgccctc gagggtggtt tcttcatccg aaggccgctt cttttgtgcg
      721 aattcggcct cagcgaggaa ttgctttgtc agctccgctc gatatcaatc ctgcgcggcg
      781 gcacattctg ccattttcac gaggtagaaa tcatcggtga ggaacccatt ttggtaaagg
      841 agattgagca tcctcacgcg accggcgacc tgggccttac tgaagggtgc ccggcctgct
      901 tcttgagccg ccttgcgggc agcctccacc atttccggag gcacttctgg cggagttcgt
      961 gcgggttgcg gctggtaatt ggggtcatac acccggaccc tcaaatccgc cacaggcaac
     1021 aagtgccgcc acaagaaggc ctgcatgcga tcctctgcgg cgacggcctc atggacgatt
     1081 tcctgatcgc cgactttggc ccgcccctca acccgtagtg gaatcgcccc atctgaatct
     1141 ttcaaagacg tctttatggt aagtccgacc atctcctgat tggcccttaa aacgacgggc
     1201 gcattggcaa atccttccgg tggatctttg agagacactt gaatgtcccc ggcaaagccg
     1261 tctttgcgaa tggcataaac cctgacggtg ttggcacttg cactacgcac ggccacggca
     1321 gagggaacaa cgcgcaacgc gaaatcggga cgcggctcgc tcagtcggag gcgatacgca
     1381 tattctgggc cacctttccg cgtggtgtca ccaagataga catagtaggc gccatcagca
     1441 ggtagcggga tcataatata ggaatccgcg tgatacgtca tttgccccat tccaggatcc
     1501 tcataatcgt cattcagcgc gatcggctta ccgctggcat ccgtgattcg gagaacggaa
     1561 tctaacgggg aatcgagccg ccgagccgtg acctcggcca ctaatgtttg ccccgctttc
     1621 ccctcaacac agaagacatc caaatctccc ggctgatcga ttcgcccatt gataatcaca
     1681 gggagcgaaa ctttctcggc ctccgcgggc ttgttgttgg gctctttttc cataatctcg
     1741 gggagggtat ccacggcaaa cggccagcga ttggagacaa ggccgttctt gaccgccgtc
     1801 acaaagtaaa cgccagatcc cgcatctgcg ggaggtagcc ttaattccgc cccttccata
     1861 ttccaacctt ttacctcggg ggtgatggat tcgcccaact tccgacctaa ggggaaaacg
     1921 ctcgtgataa atggggactc cgcaattgag atgcgataaa caaaatcttc tcgtccgcgg
     1981 aaaagggcat ccgaaatggt caagacgtac tcgccgtcct gcggtacttc gtaatagatg
     2041 acaggatcgg gatgaaaccg aaagccatcg ctgaacgcaa cttcctggcc ctgtgcgtca
     2101 taaagcgtga gcatcgactg gaaccagccg ggaacggcat ccgccatgta aggaattaga
     2161 tcccgtgtgt aagctttgat gaggagccgc tgacctttac gggcttcaaa ccggtaagca
     2221 ttcacaaaac ccggtgccac ctgtccattg acgacgcagg gaatggtaac cctgacttct
     2281 ttttcctcgg gcggacgacg ccgctcggcg gcctgttcat tgcccagtac tggaaactga
     2341 caggccttga tgggtttacg gacgtactcc ggaaactgac ccacataaaa aggaagggga
     2401 ttcgttaccc cttgcgttgt caccagccga atttctcgtg gcccaggttc ggcatccggg
     2461 gcaacagtca cttccacgat tgctaagttg acgatcgctc gattggctgg gaaattctgc
     2521 cagtcattga tgcgatcctg gatgcgttcc atcaactggc gggttgcgtc gtcgagaggt
     2581 tcattatttt tctctttttc cttggccacg cgctggagtt gttgaagctg ttcccgcatc
     2641 acggcc
//
LOCUS       DAOPTR010000546         2644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_30893_length_2644_cov_3.756663, whole genome shotgun sequence.
ACCESSION   DAOPTR010000546 DAOPTR010000000
VERSION     DAOPTR010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30893_length_2644_cov_3.756663"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..1040)
                     /gene="hisN"
                     /locus_tag="PKI05_10490"
     CDS             complement(180..1040)
                     /gene="hisN"
                     /locus_tag="PKI05_10490"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HOA52674.1"
                     /translation="MQTDLGNHLTLDRIFNVGAASEIQGRLDFAVEAARAAGEITLRY
                     FRRADLAVERKADASPVTLADREAEAYLREAIHKRFPQDGIVGEEWGTDEGSSGFRWI
                     IDPIDGTKSFIHGVPLYATLIGVEWEGRAVIGVDRIPATGEVIFAAKGRGAWYQIGEE
                     PPQPARVSRCENLAEAAWVTSEVINFDKIDRREAFHRLQSATRLFRTWGDGYGYLLVG
                     TGRIDFMIDPLMEIWDAAAIQPIIEEAGGIFTDWRGQPVFTSGNGIGSNGLLHNQVLD
                     VLRGRDNQMG"
     gene            1506..2468
                     /locus_tag="PKI05_10495"
     CDS             1506..2468
                     /locus_tag="PKI05_10495"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOA52675.1"
                     /translation="MTLCRFTCPECKQSFTMELPDDTDLAKVTCPQCQSILTVKVPSE
                     PPPIPEVPTDQEPPSQLVVPPPIPPTLPPEREASSGQIPLPSDILTQGHRNSEMASSP
                     ETSVVPPALAPYAPPTAPVSVAAEHACPLCGRSDPKYFVRGKMLYGHKVCRKCYLSFL
                     NRRQFAFAFDIVAYFILLLGVNFLEEFTVGFVLSATQAELIEQQLEAILLAFNVLNYV
                     LYALWFLFKDGFNGTSLGKAIMGLFVINEATGKRISFGGSAKRNWIIVLLGLVPFAWL
                     IIAAIMGKGYRIGDRMANTKVVWKKYAHSPVFAPNRAVLSSPGL"
BASE COUNT          564 a          805 c          675 g          600 t
ORIGIN      
        1 gattcgttcc agagtaacac aaacgcgccg agcctaccgt acggcggggg cctttttatc
       61 cgcggcgatc gacctgcccg tttgcgccgg cggctagcaa tccattcata cgggagggag
      121 cacgccgcca gatatgctgc aatccgcgcc cacgtctggg aagcgaacgg tcccaggaat
      181 catcccattt gattatctct tccgcggagc acgtccagaa cctgattatg gaggaggcca
      241 ttactgccga tcccgttgcc ggaggtgaag acgggctgac cccgccaatc cgtgaagatc
      301 ccgccggctt cttcgataat cggctggatg gccgccgcgt cccagatttc cataagggga
      361 tcaatcataa aatcgatccg tccggtgccc accaaaaggt aaccgtagcc gtcgccccat
      421 gtgcggaaaa ggcgtgttgc cgattgaagt cggtgaaagg cttcgcggcg gtcgatctta
      481 tcaaagttga tgacttcgct tgtcacccag gccgcctcgg ctaggttctc gcaccgcgaa
      541 acgcgggccg gttggggcgg ttcttcccca atctgatacc acgcgccgcg tcccttggcc
      601 gcaaaaatga cttcgcccgt cgccggaatc cgatcgaccc caatcaccgc ccgcccttcc
      661 cattccacgc cgatcaatgt ggcatacagc ggcacgccgt ggatgaacga cttggtgccg
      721 tcgatggggt cgataatcca acggaagccg ctacttcctt cgtccgtccc ccattcttcg
      781 ccgacaattc catcctgagg aaacctcttg tggatggcct ctcgcaggta ggcttcggcc
      841 tcccggtcgg cgagtgtgac aggcgaggca tcggccttcc gctccacagc caaatcggcc
      901 cggcgaaaat agcgcagcgt gatctctcca gctgcgcggg cagcctccac ggcaaaatcc
      961 aaacggcctt gtatctcgct ggcagctccc acgttgaata tcctatccaa tgtcaaatgg
     1021 ttgcccagat cggtttgcat tcaccaaaac cgccattatc gcagaaataa ccaaacacat
     1081 tgtaggggca ggcccctgtg cctgcccatg atgtggccgc gtccaccaac ccgctcgggc
     1141 aaccacaggg ggttgcccct acggacgcta acccgaccgg aagaatccaa ccactgtacg
     1201 ggcacgcccc tgtgcctgcc cacgatgtgg caatgtaacg ctgccagacg tataatcgtc
     1261 accggcaacc gccggtatga cgttcgtgag ccgatcatcc aacagcgagt ggtggccaat
     1321 cgattgaaca ggctctatgg taatcgtttt caatccctag gacgagcagc gtcgctcgct
     1381 gaaaccgtat tcaaagcggc cgtttcggca gtacgccacc cactgtaaac tcaacacgtg
     1441 gtaaaagatt acccaacacg attaacgttg tttctagtcg attgtctgtt ttcggagagt
     1501 aaaacatgac tctttgtcgc ttcacttgtc cggagtgcaa acagtccttt acgatggagc
     1561 ttccagatga taccgacctg gcaaaagtca cctgccctca gtgccagagc attttgaccg
     1621 ttaaggtgcc gagcgaaccc ccgccgattc cagaggtacc gacagatcag gaaccaccat
     1681 cgcagttggt cgtaccgcct ccaatcccac cgactttacc tccggaacgc gaagcatcgt
     1741 ccggccaaat tcctttgccg tcggacattc ttacccaagg ccatcgcaac tcagagatgg
     1801 ccagctcgcc cgagacaagc gtcgtcccgc cggcgctcgc accgtatgca ccgccgaccg
     1861 ccccggtctc ggtagcggcc gagcatgcct gtcccctttg cggccgctcg gatccgaaat
     1921 acttcgtccg cggtaagatg ctttatgggc acaaggtctg caggaaatgc tatctgtcgt
     1981 ttcttaaccg ccggcaattc gcctttgcgt tcgatattgt ggcctatttc atactgctgt
     2041 tgggagtgaa tttcctagag gaattcacgg tgggctttgt cctcagcgca acccaagctg
     2101 aattgattga gcaacaatta gaagcgatcc tacttgcgtt taacgttctt aactatgtct
     2161 tgtatgcgct ttggttcctc tttaaggatg gcttcaacgg tacttcgctc ggtaaagcga
     2221 tcatgggcct cttcgtaatc aacgaagcca caggcaaacg catttctttt ggaggatcgg
     2281 ccaaacgcaa ttggattatc gtcctccttg gtctcgtgcc gtttgcgtgg ctaattattg
     2341 ccgcaatcat gggaaaagga tatcgcattg gggatcggat ggctaacaca aaagtggtgt
     2401 ggaaaaaata tgctcacagc ccggtgtttg ctcccaatcg tgccgtttta tcatcccctg
     2461 ggttatgaag cacgccgcct cttcttttaa cttttgtctt gacgtcattt cggattactg
     2521 gtatttaccg acacattgac ggtccctgtg tcatggacat gccagcgccc ccttggcgcc
     2581 gctggtgcta tttatcgtcg atcaggagcc aaatcgcgtt gtccacccgt tggcgacgaa
     2641 ccct
//
LOCUS       DAOPTR010000547         2640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_30959_length_2640_cov_3.169826, whole genome shotgun sequence.
ACCESSION   DAOPTR010000547 DAOPTR010000000
VERSION     DAOPTR010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30959_length_2640_cov_3.169826"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PKI05_10500"
     CDS             <1..280
                     /locus_tag="PKI05_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52676.1"
                     /translation="PPPSNSFFDQLFNGAKSLAFSEDISNPLLMTTMATNYLWYFIPL
                     LIAVSLVYAATRQETMPFILRHAVRTGMWIIIFMGIIAVILQVLDAFT"
     gene            complement(296..1066)
                     /locus_tag="PKI05_10505"
     CDS             complement(296..1066)
                     /locus_tag="PKI05_10505"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOA52677.1"
                     /translation="MTEPSDVLVVVVTYNERETLPQLVEAVFNSAPQVDILVVDDNSP
                     DGTGKWCEEYARQNPRMHCLQRPAKLGIGSAVAEGLRWAIDQGYRYVITMDADLSHPP
                     EKIPELLAAIEGSNTTGADVVIGSRYVKGGTIQGWPWYRYLMSWGINSFSRWLLRLPV
                     RDCSGNFRCYRASVLAALNWEEFCSTGYSFFEEILFRLRQEGATFQEIPIRFVERREG
                     RSKMRLREALSAIAAIIRLALLGACSRLRKKTKPQHPR"
     gene            complement(1424..1864)
                     /locus_tag="PKI05_10510"
     CDS             complement(1424..1864)
                     /locus_tag="PKI05_10510"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52678.1"
                     /translation="MGCPGTVVFEKLESWTDRPGPGIRYYSGTAIYETEFTCTDQDLS
                     AHGTHLWLDLGLVRELAEVFVNGRSCGVVWAPPFRVDLAGAVRAGKNELRIEVVNFWP
                     NRIIGDASLPPEKRLTRTNIRKLTPDTALMPSGIFGPVQIGRLE"
     gene            complement(2068..2640)
                     /locus_tag="PKI05_10515"
                     /pseudo
     CDS             complement(2068..2640)
                     /locus_tag="PKI05_10515"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
BASE COUNT          586 a          783 c          682 g          589 t
ORIGIN      
        1 accaccgcca agcaatagtt tttttgatca actgtttaac ggagcgaaat cgttggcatt
       61 ctcggaggat atttccaatc cactccttat gacaactatg gcaacgaact atctttggta
      121 cttcattccg ctcctgatcg cagtcagtct tgtttacgca gcaacgcggc aagagacgat
      181 gcccttcatc cttcgccatg ccgtgcgaac agggatgtgg attatcatct tcatgggcat
      241 tatcgctgtc atcctacagg tccttgacgc gtttacctga acgatgggcc gagcgttacc
      301 tcgggtgctg aggtttggtc ttttttcgaa ggcgggaaca agcaccaaga agggccaacc
      361 gaatgatggc cgcaatcgcc gagagggcct cccgtaatcg cattttcgac cggccctcgc
      421 gtcgctcgac aaagcgaatt gggatctcct ggaaagtggc cccttcttgt cttaaacgga
      481 atagaatctc ctcaaaaaac gagtaccctg tggagcaaaa ctcctcccag ttcaaggccg
      541 ctaaaaccga cgcccggtaa catcgaaaat tgccgctaca atcgcgaact ggcaaccgaa
      601 ggagccaccg ggaaaagctg ttgatgcccc aactcatgag atagcgatac cacggccaac
      661 cttgaatggt tccgcctttc acatagcgcg aaccaataac gacatccgcc cctgtcgtat
      721 ttgacccttc gattgcggca agcaactcgg gaatcttctc cggaggatgg ctcaaatcgg
      781 catccatcgt aataacgtac cgatatccct gatcaatcgc ccagcgaagg ccctcggcaa
      841 ccgcgctccc aatgccaagt tttgccggcc gttgaaggca atgcatacgc gggttctggc
      901 gggcatattc ttcacaccac ttccctgtgc catccggcga gttgtcgtcc accaccagga
      961 tgtcaacctg gggggctgag ttgaagacgg cctccacaag ctgtggaagg gtctcccttt
     1021 cgttgtaagt tacaacaaca acaagtacgt cagaaggttc ggtcatcggc ttccgcggtc
     1081 tgcctcatgc catgtgttca ggtcaattcc tttcacgttc attctctgac cgacttaccg
     1141 agttgacaag ccgctggcac gtctactgag agagcactta gaagcaatct ctcccctcgc
     1201 ccattcggtg ggagatggat cgggaggagg gtggctctgg ttgctaccgg ttttttcaat
     1261 cgaccccggt gatcgcttgc cctcacccca accctctccc ggagggagag gtggactttt
     1321 tgttgagata gactcttaaa ggcttccgcg gcgtagatgc catgatgcga ccgccagcgt
     1381 aatccgcgct tggggaaacg tgccccagtg tcgcagttat cacttattcc agtcgcccaa
     1441 tctgtacggg gccgaaaatt cccgaaggca tgagcgcagt gtcgggcgtc aacttacgaa
     1501 tattggtccg tgttaatcgc ttttctggcg gcaaggaggc atcaccaatg atgcggtttg
     1561 gccagaagtt gacaacctca atgcggagct cgtttttccc cgcgcggacc gccccggcaa
     1621 gatcgacgcg gaaaggcggt gcccacacca cgccgcacga acgcccgttc acaaatactt
     1681 ctgccaactc ccgtaccaat cccaagtcca accacagatg cgtgccatgt gcggaaagat
     1741 cctgatctgt acacgtgaat tctgtctcgt aaatggccgt tccagagtag tatcgaatgc
     1801 ccggtcccgg gcggtcggtc cagctttcaa gcttttcgaa gacaaccgtt ccaggacacc
     1861 ccatcgggga tcaaatgtga cgcgccaagg ttcggggatt tctcgcaacg tgacccaccg
     1921 tgaggtgttt gattcctttc ggggtggatg ctctgcagac gattgccgaa atacgataaa
     1981 ccacgcccca cacggtggga actccaaggg cacggccgtc cgtccttctt tttctgaata
     2041 tgcttcggca aaagcccgcc gacccgtcac ggggtcccac agctctgggg cctttcccgt
     2101 cacgcgaaat accgccgtta agcgctctgg cttgggatta cgattggcca cgaagtaaat
     2161 gtccacctca cccactcggc gatggatgta gtccaaatcc gcctccgcat ctcccccgat
     2221 gaactcgcaa tccggcggta tgccggcgtc ttgtagtact tcgcgggctg tcttgtcagc
     2281 gatcacgcgc ccattgtccc acaactcggc aactaaccgc tgatgttccg cttcgtcgtc
     2341 gctcaaactg ggggattctt tgggccgcgg gcccacaatg gtcgcaccgt ctctggctaa
     2401 ctccgccacc ttgcggagaa cgggcagcga aatcactccg tggttaggca aaacgaggat
     2461 acgataactc ataccgtccg ggagcacgat gcggccggat tgaactgtgg cccgcgtcaa
     2521 caacgcctcc gccgtgataa cgtcgtagtc ataaccgggt aaaaccttgg cggggtccgt
     2581 tcgcttgtgt tgggcaaagt tgggaacgtg atcgccgtaa taataacaaa catcggcgac
//
LOCUS       DAOPTR010000548         2639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_30970_length_2639_cov_3.687307, whole genome shotgun sequence.
ACCESSION   DAOPTR010000548 DAOPTR010000000
VERSION     DAOPTR010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30970_length_2639_cov_3.687307"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="PKI05_10520"
     CDS             complement(<1..1063)
                     /locus_tag="PKI05_10520"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOA52679.1"
                     /translation="MRRNDIGVNRNTFPEEVRRVGCEDRPKMAAAPHSIDWSRLINYL
                     ESHRSFVLTTHIRPDCDAIGSQLALAEILRNRGCQVACINAFTIPPPLQFLDTEKQLM
                     RVDQPEASRCLEQAEILVILDTTAWAQLGDMGPVIRQFPRKKIVIDHHVSGDDLGAEL
                     FKNTEAEATGRIIAELAAQWGVALTPRLAMLLFAALATDTGWFRFNSVCAETFQLAAD
                     LARAGVRPDALYRQLYENERIGRFRLMGRAMSRVETDLDGRIIYSYIRSSDFSETGAL
                     PSDTEDIINTTLSVAGTQVAIMFIEHTPGQFKVSLRSRCHLDCCRLAGEFGGGGHKSA
                     AGATMTGSLDSVKARVLDAV"
     gene            complement(1124..2056)
                     /locus_tag="PKI05_10525"
     CDS             complement(1124..2056)
                     /locus_tag="PKI05_10525"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOA52680.1"
                     /translation="MKIFRRLEEIRGLEGSVVTIGNFDGVHRGHLRLLEVLRKAADDM
                     KLPAVVMTFDPHPAVILRGVEPGPINWLERKLSLLSQAGVDIVLAYPTDRRLLELSPE
                     AFFYEVLRDQLGARVIVEGEDFRFGRDRTGDTEMLRQLCEKEGIGCTVVPPVVWQGAP
                     VSSSRIRECLMCGDVVEAAQLMSRPFRTCGEVVRGAGRGRTLGFPTANLEKVRTLLPQ
                     PGIYAGAVPLGNQIYPAAISLGGNPTFDESQMKLEVYIVDFTGNLYGQEVQVDFLQRL
                     RDIKRFASAEELIAQMSDDVEKTREIVRRCDMIQ"
     gene            complement(2430..>2639)
                     /locus_tag="PKI05_10530"
     CDS             complement(2430..>2639)
                     /locus_tag="PKI05_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52681.1"
                     /translation="EHHPLQLTAHKRSGSFFNLTVPFRILTHKPMTAIMVNVSNMRNM
                     AAANFSTAVAGKVGALQALSSDSRT"
BASE COUNT          630 a          795 c          641 g          573 t
ORIGIN      
        1 gtaccgcgtc aagaacacgg gctttcacgc tatcgagtga tccggtcata gtggcaccgg
       61 ctgccgattt gtggccaccc ccaccaaact cgcccgcgag gcggcaacaa tcgagatggc
      121 accggctccg aaggctcact ttgaactggc cgggcgtatg ctcaataaac atgatggcca
      181 cctgagtgcc cgcgacgctg agtgtcgtgt taatgatgtc ttccgtgtca gacggtagcg
      241 cacccgtttc tgaaaaatcg ctggaccgta tatacgagta aatgattctc ccatccaaat
      301 ctgtttccac ccgcgacatg gcccgcccca taaggcgaaa ccgcccgatc cgctcattct
      361 cataaagctg acggtacagt gcatcagggc gaacccctgc ccgtgctaag tcggctgcca
      421 attgaaacgt ctcagcgcac acagaattaa agcggaacca cccggtgtct gtcgccaagg
      481 ccgcaaaaag aagcatcgcc agtcttgggg tcaaggcgac tccccattga gccgccaatt
      541 cggcaatgat gcgaccggtg gcctccgctt cggtattctt gaataactcg gcccctaaat
      601 cgtcaccgct aacatgatga tcaatgacaa ttttcttgcg tggaaattgc cgaatcaccg
      661 gtcccatatc gcccaactga gcccaggccg ttgtatccag gatcacgaga atctcggcct
      721 gctcgaggca acgactcgcc tcgggttggt caaccctcat taattgcttc tcggtatcca
      781 aaaactgcag gggtggcgga atcgtgaagg cattgataca agcaacctgg cagccgcgat
      841 ttcgcaagat ttcggccaag gccaattgac ttccaatggc gtcgcaatcc ggtcgaatgt
      901 gcgtggtcag aacgaatgat cggtgacttt ccaagtaatt aatgagccgg ctccaatcga
      961 tcgaatgggg cgcggcagcc attttcggac gatcttcaca acccacgcga cgaacctcct
     1021 ctggaaacgt gttacggttt accccgatgt cgtttcttct cacccctggt gcccctactt
     1081 gcgctgccgc gctgctcccc ttcccttttt aggacactcc accctactgg atcatatcgc
     1141 agcgccgtac gatctctcgt gtcttctcca catcgtccga catctgcgcg atcaattcct
     1201 cggccgaggc aaaccgcttg atatcacgca aacgctgaag aaaatcaact tggacttcct
     1261 gaccgtaaag attacctgtg aaatcgacaa tataaacttc cagcttcatc tgtgattcat
     1321 caaacgtcgg gtttcccccg agactgatgg ccgcaggata gatttgattc cctaggggca
     1381 cggcacctgc gtagatacct ggctggggca acagcgttcg cactttttcc aaattggcgg
     1441 tgggaaaacc aagtgtcctt cctcggcccg cgccccgcac cacctctcca caggttcgaa
     1501 atggtcgcga catcaattgg gcggcttcca caacatcgcc gcacatcaga cactcgcgaa
     1561 tccgagaact ggaaaccggt gccccttgcc aaacaacggg cggcacaacg gtgcacccga
     1621 tgccttcctt ttcacaaagc tgtcgcagca tctcggtgtc acccgttcgg tcgcgaccga
     1681 accggaaatc ttctccctcg acaatcaccc gcgcccctag ttgatcccgc aggacctcat
     1741 aaaagaaggc ctccggggac aattccagca ggcgacgatc tgtgggatac gctaggacga
     1801 tgtcaacccc ggcctgcgaa agtaaggata acttgcgttc taaccaattg atcggaccag
     1861 gctcaacccc acgcagaatt acggcgggat ggggatcaaa ggtcatgacc acagccggca
     1921 acttcatgtc atcggccgcc tttcgcaaga cctccagcag acgaaggtgt ccccgatgga
     1981 caccatcgaa attcccgatc gtgacgaccg agccttcgag acctctgatt tcttcgaggc
     2041 gtctgaatat tttcacaata gcctaccgtt gccagcgcta ctcgagatta tcaacgattc
     2101 acttcgttta cgaaaaccga aacacatcat tataaagatt ggccaaggaa tgcggggagg
     2161 gcatctcgcc gcatggtttt cttcacagtc cattgtgcgt gccagacgca agggtcgatt
     2221 caacgcaaaa tgtggcctgc cctttcgccc atcaaacgac ctcggggctc aagagaacaa
     2281 tcaaaacagc aaatacccgg attacccaaa ccctcccgtc atgcgattgc ctcatgcaac
     2341 aatctgaagt ctttcgagaa gaccctggca ggggccatgc cctcgcgtcc gtctgacgat
     2401 tatcaggggc tcgcttggtg cgatcggttt caagtcctac tatccgaaga taacgcttgc
     2461 aaggctccta ctttgccagc aactgctgtg gaaaagtttg cggccgccat atttctcata
     2521 ttcgaaacgt tgaccataat agccgtcatt ggcttgtgcg tgagaattcg aaagggcacg
     2581 gtgagattga agaaacttcc actacgcttg tgcgcggtca actgcaaagg gtgatgctc
//
LOCUS       DAOPTR010000549         2637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_31007_length_2637_cov_2.582881, whole genome shotgun sequence.
ACCESSION   DAOPTR010000549 DAOPTR010000000
VERSION     DAOPTR010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31007_length_2637_cov_2.582881"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="PKI05_10535"
     CDS             <1..1276
                     /locus_tag="PKI05_10535"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOA52682.1"
                     /translation="GHRCCQHNVSHGWPYFVEHLWMAAPGDGLAAVLYGPCRVRAKVG
                     KSGNEVTIIEETQYPFRDTITFTVRAATPTDFPIYFRIPGWCTNPQVEIASAGGAKIT
                     PEIPGEKAGWLVLWRMWQDGDRITLRLPMTVKLRRWERNHNSVSVDRGPLTYSLKIGE
                     RYVQAGGTEKWPAWEIYPTTDWNYGLVLRDDDPAAGFEVVERPWDGVSQPFTPETAPV
                     VLQTKGKQIPQWKLDQWGLVAPLQASPVRSSSPETTIELIPMGCARLRISAFPVIGTG
                     PDAVEWKEPSLPPHEASHCFEHDTVAALSDGLEPKSSFDRTIPRFTWWPRRGTEEWVT
                     YRFDTPRHVSEVQVYWFDDTGAGSCRVPASCRVEWWDGENWQPVRPDPLCGVEKDRYN
                     VLRFDPVTTTQLRLVVQLREGFSAGILEWRVK"
     gene            1510..2244
                     /locus_tag="PKI05_10540"
     CDS             1510..2244
                     /locus_tag="PKI05_10540"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOA52683.1"
                     /translation="MSTLQRSSRRCDNLISGFGLGVFLILGFTAVLFASGASAQNISF
                     QRVEIDAEPPKSPWLKLAGDFNGDGKLDIAIGGASMYLVWYVNPTWQKITIAEKGWQT
                     VSGAVGDIDGDGDVDILPGAQIWLENPLPKGDPTKGPWPVRRISNVRTHDALLADLDV
                     DGRLDIVARDQSGFNHNTGNKIHMWYQRDLDRWDYHVVDCPHGEGLAVADLDRDGDPD
                     VIIAGIWYENPGKAGGDWKSHQFTAQ"
     gene            2263..>2637
                     /locus_tag="PKI05_10545"
     CDS             2263..>2637
                     /locus_tag="PKI05_10545"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOA52684.1"
                     /translation="MAVGDINGDGRCDIILSPSEYKGQTYQVAWYEAPSDPASPEWAQ
                     HVIIPAIESVCHSLQVADMDGDGLLDIVTARMHQGAPPQEVMVLLNREKGRKWEKLVV
                     STRGSHDLLVADFDGDGRPDILG"
BASE COUNT          566 a          655 c          815 g          601 t
ORIGIN      
        1 cggtcaccgt tgttgccaac acaatgtttc ccacggatgg ccgtatttcg tcgagcattt
       61 atggatggct gcccccggtg atggtttggc ggcggtgttg tatggtcctt gccgtgttcg
      121 ggccaaggtt ggaaaatccg gcaacgaagt gacgatcatc gaggaaacgc aatacccgtt
      181 ccgggacacg atcaccttta ccgtgcgggc ggccactccg acggacttcc ccatttactt
      241 ccgtattcca ggctggtgca caaatcctca agtggagatt gccagcgccg gtggggccaa
      301 gattacgcca gaaataccgg gtgagaaggc cggatggtta gtattgtggc gaatgtggca
      361 ggacggagat cggattacgt tgcggctacc gatgacggtc aaacttcgtc gttgggaaag
      421 gaatcacaat agtgtctcgg tggaccgggg accgttgaca tattcgttaa agattggtga
      481 gcggtatgtt caggccgggg gaaccgaaaa atggccggcc tgggagattt accccacaac
      541 cgattggaat tacgggctgg tattacggga cgatgacccc gcggcagggt ttgaggtggt
      601 ggagcgtcct tgggatggtg tgtcgcagcc ctttacgccc gagactgccc ccgtggtgct
      661 gcagacaaag ggaaagcaaa tcccacagtg gaaactggat caatggggcc tggtagctcc
      721 gttacaagct agtcccgtgc gttcatcgtc tcccgagaca acgatagaac tgattcccat
      781 gggttgtgcc cggttacgga tcagcgcctt cccggtgatc ggcacaggcc ccgatgccgt
      841 cgaatggaag gaaccgagct tgcctccgca tgaggcctca cattgttttg aacatgacac
      901 ggtggcggcc ctcagcgatg gtttggagcc caagagttct ttcgaccgga cgattccccg
      961 ctttacctgg tggccccgcc ggggaacaga agaatgggtt acgtaccgtt tcgacactcc
     1021 tcgacacgtc tctgaggtgc aggtgtattg gttcgacgac accggcgcgg gatcgtgccg
     1081 ggtaccggct tcgtgtcgcg tggagtggtg ggatggtgaa aattggcaac ccgttcgtcc
     1141 cgatcctctt tgtggcgtgg aaaaagaccg ctacaatgtg ctgcgatttg atcctgttac
     1201 cacaactcag ttgcggctgg tggtgcagct ccgcgagggc ttctccgccg gcattttaga
     1261 atggcgagtg aagtaaccag cgatcgtatg ctgacatggg tttatcccct cagacaatga
     1321 tccgtcgtag tatcgtggtc atcattctaa aggcgtgatg ggtcctctca cctggacaaa
     1381 aatgggggac tcatttcgcc gggagaaacc ctgggtagtg gtttcaattt tgacagtaca
     1441 cgatgggaca ggtttgttcg cccgctacac cgcgctaaga tctcgtgggc gcgaaaggag
     1501 gaacggcaaa tgtccacact tcagcgatca agccgccgtt gtgataatct gatttctggg
     1561 tttggacttg gtgtgttcct tattctcggg ttcaccgcag ttctgtttgc gagtggggca
     1621 tccgcacaaa acatatcgtt tcagcgggta gaaatagatg ccgagccgcc caagagcccg
     1681 tggcttaagc tggccggcga ctttaatgga gatggcaagt tagacatcgc cattggtggg
     1741 gcatctatgt atctcgtatg gtatgtcaat cccacgtggc aaaagatcac cattgcggaa
     1801 aaaggatggc aaaccgtatc tggggcagtg ggtgacatcg atggggacgg tgatgttgat
     1861 atcctgcctg gtgcccaaat ctggttggaa aaccctctgc ccaagggaga tcccacgaag
     1921 ggcccatggc cggtccgtcg aattagcaat gttcgaaccc acgatgccct cctcgcggat
     1981 ctcgacgttg acggtcgatt ggatatcgtg gcgcgggatc agtcgggctt caatcacaat
     2041 acgggcaaca agatccatat gtggtatcag cgagatctcg atcggtggga ttatcacgtg
     2101 gtggattgtc cgcatgggga gggcttggcc gtggcagatc tcgaccgtga tggtgatccg
     2161 gatgtgatca tcgccggtat ttggtatgaa aaccctggta aggccggtgg tgactggaag
     2221 tcgcatcagt ttaccgcgca gtagacctgg cccgatagca aggtggctgt tggtgatatc
     2281 aatggggatg ggcgatgcga tatcatcctg tcgccgtcag aatacaaagg tcaaacatac
     2341 caagttgcct ggtacgaagc accttcggat cctgcctcgc cggaatgggc gcaacacgtg
     2401 attattcccg caattgaatc ggtctgtcat agcctgcagg tggccgacat ggacggtgat
     2461 ggcctgctcg atatcgtcac ggcccgaatg caccaggggg cgccacccca ggaagtgatg
     2521 gtcctgctca atagggaaaa aggccgaaaa tgggagaaac tggtcgtgtc aactcgtggg
     2581 tcacacgacc tgctcgtggc tgattttgac ggtgacggtc ggcccgatat tctgggg
//
LOCUS       DAOPTR010000550         2633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_31064_length_2633_cov_3.801396, whole genome shotgun sequence.
ACCESSION   DAOPTR010000550 DAOPTR010000000
VERSION     DAOPTR010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31064_length_2633_cov_3.801396"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1913
                     /locus_tag="PKI05_10550"
     CDS             <1..1913
                     /locus_tag="PKI05_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52685.1"
                     /translation="EHAPIIAERLSRWGVNCVRFHFFDRPAPNGIINASRDDTRELDP
                     EQLDRLDYFVAQLKERGIYTDLNLNVARSYKPGDGVRDYEYLGFAKALTYFDSRLLEL
                     QREYARQLLTHRNPYTGHEYREEPAVALVELVNENSLIEAWMDGRLRGTNTRKNPGTW
                     TDITASYAEDLTRLYNQWLQENVSQEVRQKIAKEAGVQEGQLIPRLSPDQFANASPER
                     FHTEARFYMDVERQYFVAMRKFLREELGVKQLIIGNSDHGHGHSGYPIVVGTSLLDVV
                     DGHVYWQHPSYIRDPQTGRNIGFRIPNTPMVLDPEHSTVVQLARTPVMGKPYTVSEVN
                     HPFPNQYACEGIPILAAYAAFQDWDGIFWYTLAHSEVVGTEPRVGGHFDLAFDPVKMC
                     QLPVGALAFLRGDVQPAKKMIARTYTLEQVRESIRLPKRSDLWPFFTPGFPQTLPLKH
                     AVRVASFDGPATASFPEINENPLTSDTGELSWWLGKDKTGCVVVNSPRWQAVIGHCRG
                     VSQGTDHLRLNVTNAFCAITLASVDEKPIADAERLLLTACSQVGNRGQRWNADKTSLE
                     DWGQSPTVIEPVTGTLTLMGVVAKRVSVQPLDAQGRPIGGPVAADKGDGGWLIPLGKV
                     VTTWYNVNVERN"
     gene            2024..2527
                     /locus_tag="PKI05_10555"
     CDS             2024..2527
                     /locus_tag="PKI05_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52686.1"
                     /translation="METIRDILRAYFVYVLYPAVVLGLFVYLIALLIFLVKRAKSTSG
                     AIRRAVGSVLPVVILVFLVSTGTDSQDWLRQQLTNLDTRHRIVLGVVAALILMETGKQ
                     LGRTDANSAVAAYAFFVSCLLAILLWVLMGGLLIELNWALFAFILTGGLHIMFRGLPG
                     WFDTPGK"
BASE COUNT          561 a          662 c          764 g          646 t
ORIGIN      
        1 tggaacacgc gcccattatt gcggaacggt tgtcgcggtg gggcgtcaat tgtgtgcgat
       61 ttcatttctt cgaccgtccg gcgcccaatg ggatcattaa cgcatcgcgg gatgacacgc
      121 gggaacttga tcccgaacag ctcgatcgtc tggactactt tgtggcccag ctaaaggagc
      181 ggggcattta cacagacctc aatctaaatg tcgcgcggtc ttacaagcca ggggatgggg
      241 ttcgggacta cgagtacctc ggctttgcca aggcgctcac atatttcgat tcccgattgc
      301 tcgaattgca gcgggagtat gcccggcagt tattgaccca ccgcaatccc tacactggtc
      361 atgaataccg tgaggaaccc gcggtggctc tagtggaact ggttaacgaa aactccctga
      421 ttgaggcctg gatggatggc cgtctccgtg gtaccaacac gcgcaagaac ccgggaacgt
      481 ggacggacat tacggccagt tacgccgaag acctgacccg cctatacaac caatggcttc
      541 aagagaacgt ttcgcaggaa gtgcgtcaaa aaatcgcaaa ggaggctggt gtccaggagg
      601 gccaacttat accacgtcta agtcccgatc aatttgccaa cgcctcaccg gaacgcttcc
      661 ataccgaagc ccgtttctat atggacgtcg agcggcaata cttcgtggcc atgaggaagt
      721 ttttacgaga ggaactgggc gtcaagcagt tgatcatcgg aaatagtgat catggccatg
      781 ggcattcggg ctaccccatt gtggtgggga cttccctctt ggacgtggtg gacgggcacg
      841 tgtactggca gcacccgtcg tatatccgtg atccccagac gggacgaaat attgggttcc
      901 gtattccgaa tacaccaatg gtgcttgatc ccgagcactc gacggtggtt caactggccc
      961 gaacacccgt gatgggtaaa ccttacaccg tttcagaggt taatcatccg tttcccaatc
     1021 agtatgcttg tgaaggaatt ccgattttgg cagcctacgc agcctttcag gattgggatg
     1081 gcattttttg gtacacgctg gctcattcgg aagtcgtggg gaccgagcct cgggtagggg
     1141 gacattttga tttggcattt gatcccgtga agatgtgtca gttgccggtt ggggcattgg
     1201 cgtttttgcg gggtgacgtc caaccggcga agaagatgat cgctcgaacc tacacccttg
     1261 agcaggtcag ggagagtatc cgtcttccca aacgaagcga cctctggccg ttctttacgc
     1321 cgggatttcc ccaaacgctt cccctgaaac acgcggtgcg ggtcgcgtcc tttgatgggc
     1381 cagcgacagc cagttttccc gaaatcaacg agaatccttt gacctcggac accggggaat
     1441 tatcgtggtg gttaggaaag gataagacag gctgtgttgt cgtcaattct ccgcgatggc
     1501 aggccgtcat cggtcattgc cgaggtgtgt cccagggtac cgatcatttg cggcttaacg
     1561 tgacgaacgc attttgtgcc attactctgg catcggtgga tgaaaagccc attgccgatg
     1621 ccgagcggct gcttctgacc gcttgctcgc aggttggtaa tcgggggcag agatggaatg
     1681 cagacaagac atctttagag gattggggtc agtcacctac agttatcgaa ccagtcacag
     1741 gcacgctgac cctaatgggt gttgttgcca aacgagtgag tgtccaaccg ttggacgccc
     1801 aaggccgacc cattggcggt cccgtcgctg ccgataaggg tgacggtggg tggctgatcc
     1861 cgttaggaaa agtcgtcacg acatggtata atgtgaatgt tgaaaggaat tgaaaaaagt
     1921 tctttcccga gacaagtcga aagcagcggg atgtgatgag tttcgtcata gcgagggttt
     1981 tttgccatcg cctcgtctgt cagcctgagt aagagaaaac accatggaga ccattcgtga
     2041 cattctccgc gcgtatttcg tttatgtgct gtatcccgcc gttgttctgg gactttttgt
     2101 gtaccttatc gccctgctca tttttcttgt caagcgggca aagtccacct ctggagcgat
     2161 ccgccgcgcc gtggggagcg ttctgccggt ggtgattctc gtctttctgg tatcgacagg
     2221 caccgactcg caggactggt tgcgccaaca attgaccaat ttggacacaa ggcaccggat
     2281 tgtgcttggc gttgtggcag cgcttatcct tatggaaact ggcaaacagt tgggacgcac
     2341 cgatgccaat tcggcggtgg cggcctatgc cttttttgtc tcgtgtttgc tggccatcct
     2401 gctatgggtc ctgatgggtg gactgttgat cgagctcaat tgggcccttt tcgcttttat
     2461 tttgacaggt ggactgcaca ttatgtttcg ggggttgcca ggttggtttg acacccccgg
     2521 aaagtaacgt tcgcggcgag accggctttt acctcgtgta gtgtgaaaat tacgaggtgg
     2581 gaaagacatt ggcggcacgg cggctgttga tcaatcggtc actgaccttg atc
//
LOCUS       DAOPTR010000551         2633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_31067_length_2633_cov_3.346780, whole genome shotgun sequence.
ACCESSION   DAOPTR010000551 DAOPTR010000000
VERSION     DAOPTR010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31067_length_2633_cov_3.346780"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1992)
                     /locus_tag="PKI05_10560"
     CDS             complement(85..1992)
                     /locus_tag="PKI05_10560"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOA52687.1"
                     /translation="MKKQTVLRAIAFLVAMIALGQFCPHSWAEPPSVVQTLSLKPIQP
                     GVEIDTPTEEEIANCTISAEKIGDYVGWVVKDGRGYVLRRFLDTNRDNRVDTRCYFRN
                     GLEVYRDIDSDGDGKIDQFRWFTTAGGKWGVDTNQDGIIDRWQRISAEEVADEAVRAL
                     TEKNSVRLRALLLSPNELKLLGLTGPLAQEVTNTLQQVEAQFQQSAAKAAALGKVKWV
                     QFSGHYPGLLPASPDGPSQDLLVYENAIAFAEVERGTIQVLLGTLVQVGSAWRLVTAP
                     QLLDEQSAVLAQNNVFFKPPSVEPVQALDTQQVSEKMQQLLDELEKLEAAAQQAQTLE
                     QQAALNAKRAEIIEALEAEASRPEDKAMWIRQLADFLTAFDQAGNYPDGAKRLAVLYA
                     RVVKNPLDKDLPAYVRFRQLTAEYALALQQPGVKYDEVQKQWLASLEQFLKDYPAAPD
                     AAEALLQIGIGREYTDDIDAAKAAYQEIIQRFPKEPEAAKARGALRRLTSEGQPFELK
                     GSDLQGGTVDVAAYRGKILIVHFWTSSCEPCKTDMAIMKDLVTRYGGKLAIVGVNLDT
                     RKEDLQAYLVEARLPWPQIHEPGGLESRPAIEYGIVTVPTTLLIDAQGRVVKRQVTGS
                     ELESEIRRLLQ"
     gene            2300..2373
                     /locus_tag="PKI05_10565"
     tRNA            2300..2373
                     /locus_tag="PKI05_10565"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2334..2336,aa:Ile,seq:gat)
     gene            2500..2572
                     /locus_tag="PKI05_10570"
     tRNA            2500..2572
                     /locus_tag="PKI05_10570"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2533..2535,aa:Ala,seq:tgc)
BASE COUNT          547 a          734 c          699 g          653 t
ORIGIN      
        1 gctgtgttga gcctgtcttt gtagtccgta acaatatgca cggttgatac gcagtcgtgc
       61 ggtcattctg gaccatcgtt caagttattg gagcaaacgt cgaatctcgg attcaagctc
      121 tgatcccgtc acctgacgtt tgaccacgcg tccctgggca tcaatcagca aagtggtcgg
      181 cacggtgacg attccgtatt cgattgccgg acgactttcc agtcctcccg gttcgtgaat
      241 ctgtggccaa ggaagacgtg cttcaacaag ataggcctgc aaatcttcct tccgggtgtc
      301 gagattgacc ccgacaatgg ccagcttccc gccatatcgc gtgacgaggt ctttcatgat
      361 tgccatgtcg gtcttgcacg gttcgcacga actggtccag aaatggacaa tgaggatttt
      421 tccgcggtac gctgccacat cgactgttcc cccttggaga tcgctgcctt tcagttcgaa
      481 aggctgacct tcgctcgtca accgccggag ggcaccgcgg gccttggccg cttcgggttc
      541 cttgggaaac cgctggatga tttcctggta tgccgccttg gccgcatcga tgtcatcggt
      601 gtattcacgt ccgatcccga tttgaagcaa agcctcggcg gcatcggggg cagccggata
      661 gtcttttaga aattgctcaa ggcttgccag ccattgtttc tgcacttcgt cgtactttac
      721 acctggctgt tgaagtgcca atgcatactc cgctgtcaac tgccgaaatc gcacataggc
      781 cggcaaatct ttatcgagag gattcttaac aacacgagcg taaagcactg ccaggcgttt
      841 ggccccatcg gggtagttcc cagcctggtc aaaggccgtg aggaaatcgg ccaactgtcg
      901 aatccacatg gctttatctt cgggacggct ggcctcggcc tcaagggcct cgataatctc
      961 tgcccgtttg gcattgagtg ccgcctgctg ttcaagcgtt tgtgcctgct gggccgccgc
     1021 ttccagtttc tcgagctcat ccaacaactg ctgcatcttt tcggaaactt gttgcgtgtc
     1081 caaagcctgg acgggttcca ccgacggggg cttaaagaac acgttgttct gcgccaagac
     1141 cgcgctttgc tcgtccaaaa gttgcggagc cgtcaccaat cgccatgcgg aacccacttg
     1201 gacgagcgtt cccaagagaa cctgaatcgt ccctcgttcc acctccgcga aggcgatggc
     1261 gttttcgtag accagaagat cctggctggg gccgtcgggc gaagcaggca acagtcccgg
     1321 gtagtggcca ctgaattgca cccactttac ttttcccagt gctgccgcct tggcggcaga
     1381 ttgttgaaat tgtgcctcga cctgttgcag ggtattggtt acctcttggg ccagagggcc
     1441 ggttaagccc agcaatttca attcatttgg cgaaagtaaa agtgcccgca gacgtacact
     1501 attcttttcc gtcaaagcgc ggacggcttc atcggcaacc tcctctgcgg aaatccgttg
     1561 ccaccgatcg atgataccat cctgattcgt atcgactccc catttgccac ccgccgttgt
     1621 gaaccagcgg aattgatcga tctttccatc gccgtcgctg tcaatatccc ggtaaacttc
     1681 cagtccgttt cggaagtagc accgcgtgtc cacccggttg tcccgatttg tatcaagaaa
     1741 tcgccttaag acatatccac gcccatcttt gacgacccaa ccgacataat caccaatttt
     1801 ttctgctgaa atggtacaat tggcgatctc ctcctccgtg ggagtatcga tctctacccc
     1861 gggctggatt ggtttcaagc taagtgtttg aaccacgctg gggggctctg cccacgagtg
     1921 cggacaaaac tgccccaggg caatcatagc tactagaaag gcaattgccc gaagcacggt
     1981 ttgcttcttc atgtaacacc tccgtttgtt ccagatcgac tgctattcca aacagctcag
     2041 tttttctacc agttctgggg ggaccggcag gcgaaacgcc ttggttcgcg cgccccttgc
     2101 aaacactgct aacaatcaaa gcttgaggtg ccaaaacgac ggcaccctac ggcatcgccg
     2161 gatcgggaca ggagtggttt ctcatttgtg gttcttcact cacccggtaa cctggacttt
     2221 acagtgttac gggctgcgtt acaatagtaa ttgttctgtt gagagtatgg gcaacaggct
     2281 gttagcccag gtgagacaag cgcgcatagc tcagctggtt agagcgcgtc cctgataagg
     2341 acgaggtcct tggttcgaat ccaagtgcgc gcaatgggcg agatcggtga tggtcaacgc
     2401 aggaggggtg accaattcgt atggcgaacc gaaagatccc ggtttcgcaa ggtgcaggtt
     2461 tttctaatct cggctatccg caatcattgt aatgaccgtg gggatgtagc tcagttggga
     2521 gagcgcctgc tttgcaagca ggaggtcgtc ggttcgagtc cgatcatctc caaatcctta
     2581 ttggtgggca ggtctgctgt accgcaagtg aatggcttga acttgcgcat cgt
//
LOCUS       DAOPTR010000552         2633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_31068_length_2633_cov_3.273468, whole genome shotgun sequence.
ACCESSION   DAOPTR010000552 DAOPTR010000000
VERSION     DAOPTR010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31068_length_2633_cov_3.273468"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..1405
                     /gene="pgk"
                     /locus_tag="PKI05_10575"
     CDS             14..1405
                     /gene="pgk"
                     /locus_tag="PKI05_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOA52688.1"
                     /translation="MALTKEKMEAWLRRILGVDKVTMGLLDYIAGIPRVKDLTDVPAG
                     TPVLVRGDLDCKPGAKVGEGDIRLRSMKETLLLGRDRGWIQVIFGHIGRDPQLSLEKV
                     WKRLSEIMECEIVFIRDWFAPETLKIRQEVVDAIKAAKPGDIILLENTRKYDIERVLW
                     KAKPADIPNLVEKLTNVANEFAEKIAKVYVHEAFSAGSLDTSSVVVTAAMEKVALGEY
                     AYGEFSGPLADCLNAQLVISSGLKIDKLDDLEAMISRGKIRWVFTAGSLAMGLIKAAY
                     QLDGKDFCIGVAEDPAHKDQPYYIPPERIEQAKKMIASGRAQGIQFVMPVDFVLQDGR
                     VATTLSPGDQQFDVGPATSELFERKIGEFLDWAKTLKEPAVAFHNGVFGKFEDPKFEN
                     GTRRFMAQLKRMKDAGVRVYVGGGEGGTALEKYGQPDWVTHNFTAGGTVLNALGSEPV
                     PFLVALHMAANKK"
     gene            1713..>2633
                     /locus_tag="PKI05_10580"
     CDS             1713..>2633
                     /locus_tag="PKI05_10580"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOA52689.1"
                     /translation="MASLRAELGRDLVALLLLGVVLFFGASLITFDPADPPSDLVFPP
                     HEQVTNVCGPVGATVAFHALEFLGIGAWYAVLSLSILTIVLLLRQPLTRPNTRVFGWV
                     LSIFGLTGLADCVIPNWSPGPVIGPGGYVGVALRWILEQKLGFAGTVATLFAGFSIGF
                     LLCTELSLVRTVLTAVLWPLKGIVKGLGEVASGYGLGRPTSEAKAQDSAVRVKRPGEL
                     AEVPSGQAKDAAPQGNEPADTDTSQRGPLIKTPAATKEDQPQHAETVQKPEVTDRKSG
                     ELRIRKPTRPPKEELLDVIDSSTDERRHFNY"
BASE COUNT          590 a          691 c          782 g          570 t
ORIGIN      
        1 gggagagtga agcatggcgc tgacaaaaga gaagatggaa gcctggttgc gacggattct
       61 cggtgtggac aaggtcacca tgggccttct cgactacatc gctggcattc cccgggtcaa
      121 ggacctcacg gatgttccag cggggacgcc tgtgctcgtt cgcggtgacc tcgattgcaa
      181 gcccggggca aaggttggag aaggtgacat tcggctccgc tccatgaaag aaaccctcct
      241 cctcggacgc gatcgggggt ggattcaagt gatctttgga cacatcggcc gcgatccaca
      301 actgagccta gagaaggtat ggaagcggtt gagcgagata atggagtgcg aaatcgtctt
      361 tatccgcgac tggtttgccc ccgaaaccct gaagatccga caagaggttg ttgacgccat
      421 caaggctgcc aagccgggcg acatcatctt gttggaaaac acccgcaagt atgacatcga
      481 gcgtgtcttg tggaaggcca aacccgctga catcccgaac ctcgtcgaga aacttaccaa
      541 tgttgccaac gaattcgcgg aaaagatcgc aaaggtttat gtgcatgaag ctttttccgc
      601 cggaagtttg gacacttcct cggtggttgt cactgcggcc atggaaaaag tcgccttggg
      661 cgaatatgcc tacggtgaat tctcaggccc cttggccgac tgcctcaacg cccagcttgt
      721 gatttcgagc ggtctaaaga tcgacaagct ggacgacctc gaggccatga ttagccgggg
      781 caagattcgc tgggtgttca ctgctggctc gctggcgatg ggtttgatca aggctgccta
      841 tcagcttgac ggtaaggact tctgcatcgg tgtggccgag gatcccgccc acaaagatca
      901 gccatattac attccgccag agcggattga acaggccaag aagatgatcg ccagtggcag
      961 ggcccagggc atccaatttg tgatgccggt ggattttgtc ctgcaggatg gccgtgtcgc
     1021 gacaacgctt tcgcccggtg accagcaatt cgatgtcggc cctgcaacct cagagctgtt
     1081 cgagcggaag attggcgagt tcctggattg ggcaaaaacg ctcaaggaac ccgccgtggc
     1141 cttccacaac ggtgttttcg ggaaatttga ggacccgaaa ttcgagaatg ggacccggcg
     1201 attcatggct caattgaagc ggatgaagga tgccggtgta cgggtgtacg tcggcggcgg
     1261 tgaaggcggc acagccttgg aaaaatacgg ccagccggac tgggtcaccc acaacttcac
     1321 ggcaggcgga accgtgttga acgccctcgg cagcgaaccc gtgccgttcc ttgtggcgtt
     1381 gcacatggcc gcaaataaga agtaacgatt gcttatagca caattaaccg acgtaagcac
     1441 gctggactcc aaccaccgct tcggcggtgg tttttttatg ggccatttgt gactttgtga
     1501 aaagtggaac acaagagcaa acagcgctga tggcaacgct ctcacggcct caagtggctt
     1561 tgcgaaatat gtgagatgtg acgaaaatac ttgcccggcg agttggacgg aaatgagtgt
     1621 gggctcgtac gcggccggtt cgaaacagga tgtagcaggc gtcgagttgc gacggggcta
     1681 agcgtacgtt tttgtccaag cgaccaatcg tgatggcatc acttcgagcg gaattgggcc
     1741 gtgatcttgt ggcactcctg cttttgggag tcgtcttatt ctttggcgcc agcttgatca
     1801 cattcgaccc cgccgatccc cccagcgact tggttttccc accgcacgag caggtgacaa
     1861 acgtctgcgg ccctgtgggg gcaaccgtcg cgtttcatgc cctggaattc ctcgggattg
     1921 gggcctggta cgcggtgtta tcgttgtcaa ttttgacgat tgttctgctg ttacgacaac
     1981 cgctgacgcg accgaatacg cgtgtgtttg gttgggttct ctcgatcttt gggctgacgg
     2041 gactggccga ctgtgttatc cctaactgga gtcccggacc tgtcatcggc ccaggcggtt
     2101 atgtcggggt tgcccttcgc tggatcctgg aacaaaaact aggatttgcc gggacggtcg
     2161 ccacgctgtt cgccgggttt tcgattggat ttctcctttg caccgaactg tcgctggttc
     2221 gaactgttct cacggcagtg ttgtggcctc ttaagggaat cgtgaaagga ctgggcgaag
     2281 tggccagtgg atacgggctt ggacgtccca catctgaggc taaggcacaa gattccgcgg
     2341 tacgggttaa gcggcccggc gaattagccg aagtgccgtc tggccaggcg aaagacgcgg
     2401 cacctcaagg aaacgaaccg gctgatactg atacttctca acgtggcccg ttgataaaga
     2461 cacctgccgc gacgaaagaa gaccagccgc aacatgcaga aaccgttcaa aaaccggaag
     2521 taactgatcg caagtcgggt gaacttcgaa tccgcaagcc cacacgcccg ccaaaagaag
     2581 agcttctcga tgtcatagat tcgtcaaccg acgaacgacg ccacttcaac tac
//
LOCUS       DAOPTR010000553         2626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_31153_length_2626_cov_3.760016, whole genome shotgun sequence.
ACCESSION   DAOPTR010000553 DAOPTR010000000
VERSION     DAOPTR010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31153_length_2626_cov_3.760016"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..777)
                     /locus_tag="PKI05_10585"
     CDS             complement(160..777)
                     /locus_tag="PKI05_10585"
                     /inference="COORDINATES: protein motif:HMM:NF020279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly factor Coa1 family
                     protein"
                     /protein_id="HOA52690.1"
                     /translation="MSQFEENSRSRDFGETVPPERSQRRGCLWILVFVGVLVIIGCVA
                     LCLGVFAVVLSMGKEPYQMAVRLVTQDAQVVEKLGKPIEVASWFPQGSIHVRGDQGDA
                     NLTFQIRGPQGKATVNVVARRIKGKWGLTSLDVTYPDGTRQPIDVSAEGDSELDAPQW
                     QPGAQVPTESTQPAGEEPSQSEDQGREEGPAPPSLEVKIPELPKL"
     gene            1001..2569
                     /locus_tag="PKI05_10590"
     CDS             1001..2569
                     /locus_tag="PKI05_10590"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOA52691.1"
                     /translation="MVRSDAPVPPGNSESQATEPDDRGTTGRTRGLWGFLSRLFSRTP
                     EGIESTLEWLMRVSEKWEEKAAPSPSVIVAAGLVMEVMMHFGYREAAWRIGRWLQERQ
                     LPDGSLSESRAAEPVFSATAAAARGWLAALPELNQFEGAAQKACVFLRQWVGEEGRLL
                     PPRNRAWNYEPTTLFNEPPDLSPLLWAGRRWPDTDWTTVVLRAIDYWRHHGHRRVPAE
                     QSSAIVRRAIFFLDWGHRQDAIPLVEKLDRRQTAAGAVPEQDNGSVILLATVAQAALL
                     WFRMFNETRGEQAYRFVEKRRLPDGSFPRSARGTSENDREPDPLAAKYFLEATLWRVR
                     CAYRGRIKNEEPLDAEDPRLLAVRSLVATMPQGSSLADLGCGTGRYLKFLSNWFPHHQ
                     WFGIDFVPEALRQVPRGIKTIEGNLLRIPVSDQSFHAVFSVDAVSHALLPRQAVLEMA
                     RVIRPGGYILVIDRQQKSRSLGADPWEQPVTAEQIVDWLRPVARQTKVQQFPISAGTL
                     GRTPYFAVLIQRGR"
BASE COUNT          529 a          760 c          772 g          565 t
ORIGIN      
        1 agcttgtgaa acctaccgac agccggacct ttggcgacaa cttatccaga acgggattac
       61 tcaggattgg tcgtggcggc acagcgctcc gcggtacatt gagatgtacg aagacaccct
      121 ccgccgagtc cgcggcacta atcagtagag gctgtcccct cacagcttgg gaagttcggg
      181 gatttttacc tccagtgacg gcggagccgg accttcttcc ctaccttgat cttcggactg
      241 ggatggttcc tcgcccgcgg gctgggttga ctccgtggga acctgggcgc cgggctgcca
      301 ctgaggggca tccaactccg aatcgccttc cgcactaacg tcaataggct gtcgggtgcc
      361 atcggggtac gtgacgtcga gcgaggtcaa gccccatttt cctttgattc tccgcgcgac
      421 gacattgacg gtagcctttc cttgtggtcc ccgaatctgg aacgtcaaat tggcgtctcc
      481 ttgatcccct cggacgtgga tgctcccttg cggaaaccag ctggcaacct caatcggctt
      541 ccccagtttt tccacgacct gcgcatcttg ggtgaccagg cgcaccgcca tttgataagg
      601 ttctttgccc attgaaagga ccacggcgaa cacgcccaaa caaagggcaa cgcaaccgat
      661 gatcacaagg acgccgacaa agacgagaat ccaaaggcaa ccgcggcgct gcgatctctc
      721 gggcggaact gtttccccaa aatccctgga ccgcgaattc tcctcaaact gactcattgg
      781 aaaaggctcc ttatgtgagg tggcacaact tttcttgaga ttcggcaaac cagtgcttat
      841 cataaggttc accggccccg aaaagaaagg ccattttgtc tcgggagata aaaccggttg
      901 gcaaaaggct tcacgaatgc gacacaaatg gcccgactga gggcttcata ggatggtccc
      961 cagtccccgc caattggcga ggaaactcga ggatgccgcc atggttcgat cggatgctcc
     1021 cgtaccacct ggtaattccg aatcccaggc caccgaaccg gacgatcgcg gtacaacggg
     1081 ccgaacccgg ggattatggg gctttctttc tcgccttttc agtcgcactc cagaggggat
     1141 cgaatccact ctggagtggc tcatgcgggt gtcggaaaaa tgggaggaaa aggccgcgcc
     1201 ctcgccgtcc gtcatcgtgg ccgcgggcct ggtcatggag gtgatgatgc acttcggcta
     1261 ccgcgaggct gcctggcgga tcggtcggtg gcttcaagag aggcagcttc ccgacgggtc
     1321 gctgtcggag tcccgggctg cggagccggt tttctctgct acagcggctg ccgcacgcgg
     1381 ttggctggcg gccctgccgg aactaaacca gttcgagggg gcagcccaga aggcgtgtgt
     1441 tttccttagg caatgggtgg gagaggaagg gcgcctcttg ccgccgcgta atcgcgcctg
     1501 gaactacgaa ccgacgacac tgtttaatga gccgcccgat ttgtcgcccc ttttatgggc
     1561 aggacgccgt tggcccgata cggattggac gaccgtggtt ctaagggcca tcgattactg
     1621 gcgtcatcac gggcaccgcc gtgtcccggc tgagcagagc tcggccatcg ttcgccgggc
     1681 catcttcttt ctcgattggg gccaccgcca ggatgctatt ccgttggttg aaaaactcga
     1741 tcggcggcaa accgccgcgg gggccgtacc tgaacaagac aacgggtcgg tcatcctctt
     1801 ggccaccgtt gcccaggcgg cgctcctttg gtttcgcatg ttcaatgaaa cgcggggtga
     1861 gcaggcgtac cgattcgtgg agaagaggcg actacctgat ggaagttttc cgcgaagtgc
     1921 ccggggcact tcggaaaatg atcgtgagcc tgaccccttg gcggcaaagt attttcttga
     1981 ggcgacgctt tggcgggtcc gatgtgctta tcgaggacga attaaaaacg aagaacctct
     2041 cgatgcggaa gatcctcgcc tgcttgcggt ccgttctctg gttgcgacca tgccccaagg
     2101 gagctccttg gccgatcttg ggtgtggaac cggacgttac ctaaagttcc tgtcaaattg
     2161 gtttccccat catcagtggt tcggaattga tttcgtgcct gaggcacttc gacaggtacc
     2221 acgcggaatc aaaaccatcg aaggcaacct actgcgaatt cccgtttccg atcagagttt
     2281 tcacgccgtc ttttcggtgg atgcggtttc ccatgccctc ttgccgcggc aggccgtgct
     2341 tgaaatggcc cgcgtgatcc ggccaggtgg atatatcttg gtcatcgacc gtcagcagaa
     2401 gtctcgcagt ttgggagcag acccctggga gcaacctgtg actgccgagc aaatcgtgga
     2461 ctggttacgg cccgttgccc gacaaacaaa ggtgcaacag ttccccatct cagcgggaac
     2521 ccttggccga actccttatt ttgccgtgct gattcagagg ggccgatgaa ttaagagcct
     2581 atcccaaaaa gtccccctct ccctctggga gagggttggg gtgagg
//
LOCUS       DAOPTR010000554         2625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_31177_length_2625_cov_2.466148, whole genome shotgun sequence.
ACCESSION   DAOPTR010000554 DAOPTR010000000
VERSION     DAOPTR010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31177_length_2625_cov_2.466148"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1514
                     /locus_tag="PKI05_10595"
     CDS             <1..1514
                     /locus_tag="PKI05_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52692.1"
                     /translation="NHPWVTHYVRQPEENPCFVPLDEEKRAMCEHCTRREFLGTGVLS
                     ALAASSTWNYTWASQAETAQPLGKVRVGVLFTGSPGPADRGWNADEGHFEKMQTILSD
                     VQNKLSNVELIIGQSQNPQQTAAFLDQIGEGVPVLAINLNIFALTRLVGPLLERRHPM
                     AVFSLPASGHDWMYPPRWQRAGHRVTLFATSDMNEIEHAIRLLRVIPMLKQTRILLFP
                     PARGTAPAQSPEMIKERLGAEVLAVEQAAFDEMMAAVEDDAVREEYDRWVKGAKQIVE
                     PTEEDIMKAARVSVALSRLVEKYQAQGLAVGTCMGWLPRGFPCLGFTRLRDRGIPAAC
                     EGDMDSLLTMLLFQYAFDKPGFQGNATFDTSRNVLWTAHCTGPLKLDSVNGPEHPYLL
                     RGHSEVGGSGCVPEVQYRVGETITRMKFVNLDTILVSTAKIIEVPEKSVHACRTQIVT
                     QVQDAATMVANWSSALETEDAMTLLHRVVFYGDHMESVRHLARLMGWKVIQEG"
     gene            complement(1630..>2625)
                     /locus_tag="PKI05_10600"
     CDS             complement(1630..>2625)
                     /locus_tag="PKI05_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOA52693.1"
                     /translation="AHFDWGPLDVDDPAMGDTQLTDWAIGFLQQKHERPFLLAVGYIR
                     PHLPWYVPRKYFEHFPLNKIVLPQVKEDDLDDVPPVGQRMANPKGDHAKVLATNNWEK
                     AVQGYLASIEFVDGQVGRVIEAFDKSGYADNTIVVLWGDHGWHLGEKLHWRKFTLWEE
                     ATRVPLIISAPGVTQPGSRCERTVSLLDLYPTLCDFCGLKIPDHVEGMSIRKLLENPQ
                     AEWNRPVITTHGYMNHAVRSERWRYIRYHDGTEELYDHEKDPMEWTNLANDPQYASVK
                     AEFAKWLPKVNREEGPKAGQEEKRPRAKRPSAQQTSAWPDGLRAYFAREWYGEFN"
BASE COUNT          551 a          716 c          770 g          588 t
ORIGIN      
        1 acaatcatcc gtgggtaacg cattatgtac gccagcccga ggagaatccg tgtttcgtcc
       61 cacttgatga ggagaaacga gccatgtgtg aacattgtac gcggcgggag tttttgggga
      121 ctggcgttct cagcgccttg gcggcgtcca gcacctggaa ttacacgtgg gcctcgcagg
      181 cggagacggc acaaccactc ggcaaggtgc gtgttggcgt gctatttacg ggttcaccgg
      241 gtccggctga tcggggatgg aacgccgacg agggacattt tgagaagatg cagacgattc
      301 tttctgacgt ccagaataag ctcagtaatg tagagctgat cattgggcag tcacagaatc
      361 ctcagcaaac agccgctttt ctcgatcaaa tcggggaagg cgtgccggtt ttggcgatca
      421 acctcaacat tttcgcgctc acgcggttgg tcgggccatt gctggaacgg cgccacccta
      481 tggcggtttt ctctttgcct gcgagtggtc acgattggat gtatccgccg cggtggcagc
      541 gggccggaca tcgggttacg ctattcgcta cgagtgacat gaatgagatt gagcatgcga
      601 ttcgcttgtt gcgtgtcatc ccgatgctta agcagacccg catcctgctc ttccctcctg
      661 cccgtggtac ggcacctgcc cagtcacctg aaatgatcaa ggagcgactt ggggcagaag
      721 ttctcgccgt ggagcaggcg gccttcgatg agatgatggc ggctgtagaa gatgacgctg
      781 tacgagagga atacgatcgc tgggtcaagg gtgcgaagca gatcgttgag ccgacagaag
      841 aggacatcat gaaggcggcc agggtcagtg tggcgctcag caggcttgtg gaaaaatatc
      901 aggcccaggg attggcggtt ggaacgtgca tggggtggct cccccgtggt ttcccgtgtt
      961 tggggtttac acggctgcgc gatcgtggca tacccgctgc atgcgaaggc gacatggatt
     1021 cactgttgac catgctcctc ttccaatacg ccttcgataa gcctggattc caagggaatg
     1081 cgacgtttga cacatctcgc aacgtccttt ggacggccca ttgcacgggg ccgctcaagc
     1141 tggacagtgt caatggcccc gagcatccct acctactccg cgggcactcc gaggtaggcg
     1201 gcagtggttg cgtgcccgag gtgcagtatc gtgtgggaga aaccatcacg cggatgaagt
     1261 ttgtgaatct cgatacaatc ctggtcagta cggccaagat catcgaagtg ccggaaaaat
     1321 ccgttcacgc ctgccgcacg caaattgtga cgcaagtgca ggatgccgcc accatggtcg
     1381 ccaactggag cagcgccctg gaaaccgaag acgccatgac acttctacac cgggtggtat
     1441 tctacggcga ccacatggaa agcgtccggc acttggcgcg gctcatgggc tggaaagtca
     1501 tccaagaagg ctgaactgct agacagtgaa cccgatcact gtcgatcagt ctggcggcag
     1561 ttgattgtcc acttctgaag accgggtccc gtgttcagga cccggtcttt tttgcacata
     1621 atgccaccat caattgaatt cgccgtacca ttcgcgggca aaataggccc gtagcccgtc
     1681 gggccatgcc gaggtttgtt gagccgaagg gcgtttcgcc cgcgggcgct tttcttcctg
     1741 ccctgccttg gggccttctt cccgattgac cttgggcaac cacttggcaa attccgcctt
     1801 aacggaggca tattgcggat cattggccag gttcgtccat tccatggggt ctttttcgtg
     1861 atcgtaaagc tcttccgtgc cgtcatgata ccgaatatag cgccagcgtt ccgagcgaac
     1921 agcgtgattc atgtagccat gggtagtaat gaccgggcga ttccactcgg cctgtggatt
     1981 ctccagcagc ttccggatgc tcatgccctc gacatggtcc ggaatcttta ggccacaaaa
     2041 gtcacataat gtggggtaga ggtccagcag actaactgtg cgttcgcacc gactacctgg
     2101 ttgtgtcact cccggggcgg aaataatcag agggacacgg gtggcttcct cccatagggt
     2161 gaactttcgc cagtgaagct tttcaccgag atgccatccg tggtcacccc acagtacgac
     2221 gattgtatta tccgcatagc cactcttatc gaaggcctcg atcacacggc cgacctggcc
     2281 atcaacaaac tcgatgctgg ccaagtagcc ctggacggcc ttttcccagt tgtttgttgc
     2341 caggaccttt gcgtgatctc ccttcggatt ggccatcctt tgtccgacgg gcgggacatc
     2401 gtcgagatcg tcctccttga cctgcggcaa gacgatctta ttcagaggga agtgttcaaa
     2461 atacttacgt gggacgtacc aagggagatg cggtcggata taccccacgg cgagcaagaa
     2521 aggtcgctcg tgcttctgct ggagaaatcc aatggcccaa tcggtgagtt gggtatcccc
     2581 catggccggg tcatcgacat cgagtggccc ccaatcaaaa tgggc
//
LOCUS       DAOPTR010000555         2624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_31189_length_2624_cov_2.602958, whole genome shotgun sequence.
ACCESSION   DAOPTR010000555 DAOPTR010000000
VERSION     DAOPTR010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31189_length_2624_cov_2.602958"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(304..2286)
                     /locus_tag="PKI05_10605"
     CDS             complement(304..2286)
                     /locus_tag="PKI05_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOA52694.1"
                     /translation="MMADALRIGVYVCHCGTNIAGVLDTADLARFASGLPHVHVARDY
                     KYMCSEPGQELIRQDIREYGLNRIVVAACSPHLHEATFRKVLQEAGLNPYLLAIANIR
                     EHVAWITADRESGQKRARAQIAAAVGRVARQRPLEPSSYPVTPRVLVVGGGIAGIQAA
                     LTLADAGKEVILVEREPTIGGHMAKFDKTFPTLDCAACILTPKMSAVKEHPRIRLLTY
                     SEVESVQGSAGRFKVKVRRRPRYIREDLCVGCLQCIEACVFKALKIPNEFDLGLAKRK
                     PIYLPFPQAVPPVPVIDPQTCLRLRTGKCKQTCVEACADRQAIDFEQQEWIEEYEVGA
                     IIIATGFRTFDPTPLAYYGYGRFPEVYTALEVERLLNASGPSGGEIVLRDGATPRVVG
                     IVHCVGSRDHNYHPYCSRVCCMYALKLAHLIRERCGAEVYNFYIDIRAPGKAFEEFYE
                     RTQKEGVHFVRGKVGEVERLSTNGDGLRLMVTVEDSLLGRVRKIPVDMLILAVALEPA
                     EGAEQVRQLFHISCSSEGFFLEKHPKLAPVNTASAGVFIAGACQGPKDIPDTVAQADA
                     AAAQALALIDRGVVEAEPNIAWVDENVCSGCRVCVSLCPFDAITPDEEKQVAVIDPTL
                     CKGCGTCVAACPSGAAHQDLFSTEEILAELEGILAQ"
     gene            complement(2352..>2624)
                     /locus_tag="PKI05_10610"
     CDS             complement(2352..>2624)
                     /locus_tag="PKI05_10610"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOA52695.1"
                     /translation="QHRENSYCCGAGGGRIWMKDRPGIRERPAETRVKEALSLGNVED
                     LVVACPKDLVMFQDAVKSLGAEDRLRVIDVASLLAQAISPDIKSQD"
BASE COUNT          625 a          779 c          676 g          544 t
ORIGIN      
        1 ataccctccc attgttccca atcgcgcgat gcacctttcc catcgcgcat aagaaggaaa
       61 gtgacttggg atttgactag gtactattgt aatgttcgtc gcagtgaaag aaaccaccgt
      121 tagcacgtgg ggtttgaata ggaagtgtcg gcgaaacctc gcctcgtccg cggacgggaa
      181 gaacttgccg ccaaagacag tcggctagca gaatttgaag cagtccggac gccactcaac
      241 taagaaaccg gctcgcctcc tctaaaagtg ctcgatgggg tcacaccatg cttcgacgtt
      301 gacctattga gccaagatcc cctcaagttc ggccaggatt tcctccgtgg agaacaagtc
      361 ctggtgggcc gcgcctgatg gacaagcggc tacgcacgtg ccgcaccctt tgcaaagggt
      421 tggatcaatg accgccacct gtttttcttc gtcaggcgta attgcatcaa atgggcaaag
      481 cgaaacgcaa acacggcagc cagagcagac gttctcgtcc acccaggcga tattcggttc
      541 ggcctcgaca acaccccgat caataagcgc gagggcctgg gccgctgccg catcagcctg
      601 ggccaccgtg tccggaatat ccttcggccc ttggcaggcg cctgcgatga acacgccagc
      661 gctagcggtg ttaaccggag ccaacttagg atgcttttcg aggaagaaac cttcagagga
      721 gcacgatatg tggaacagtt gcctcacttg ctcagcgccc tcagcaggtt ccagggccac
      781 agccaaaatc aacatgtcca caggaatctt gcggacacga cccaatagac tgtcttccac
      841 ggtgaccatc aagcgaagtc cgtctccatt tgtggaaagc cgctcgacct cgccaacctt
      901 gccccgcaca aaatgaacac cctccttctg ggtccgttcg taaaactcct cgaaggcctt
      961 accgggagca cggatatcaa tgtagaagtt gtaaacctcg gctccgcatc gttcgcgaat
     1021 caagtgggcc aacttcaacg cgtacataca gcagacgcga gaacaatagg gatggtaatt
     1081 gtggtcgcgg ctgccgacgc agtgaacgat tccaaccact cgcggtgtcg caccatcccg
     1141 taagacaatt tctccccccg acggtccgga agcattcagc aagcgttcca cctccagcgc
     1201 ggtgtacact tccggaaagc gtccatagcc ataatacgcg agaggcgtag gatcgaatgt
     1261 tcggaagcct gtggcaataa taatggcccc aacctcgtac tcttcgatcc attcttgttg
     1321 ttcaaagtca atcgcctgac ggtccgcaca agcctcgacg cacgtttgct tgcacttgcc
     1381 cgtccgaaga cgcaaacagg tttgcgggtc aatgacgggt acgggcggca cagcctgcgg
     1441 aaacggcaga taaatcggct tacgtttagc caggccgaga tcaaattcat tcggaatctt
     1501 gagggccttg aacacgcagg cttcgataca ttgcagacac cccacgcaaa ggtcttcccg
     1561 aatgtaacgg ggccgccggc ggaccttgac cttaaaccgc cccgctgaac cttgaacgga
     1621 ttccacttcc gaataggtca atagtcggat tcgtggatgc tccttaacag cggacatttt
     1681 cggagtcaaa atgcaagccg cacaatccaa ggtggggaat gtcttgtcga acttggccat
     1741 gtggccgcca atcgtcggct cgcgctcgac gaggatgacc tcttttccag cgtcggccag
     1801 agtcaacgcg gcctggatcc cagcgattcc acctccgacc acaaggaccc tgggcgttac
     1861 ggggtaagag ctgggctcga gtggtctttg acgggcaacc cgtcccacgg ctgcggcaat
     1921 ctgagcgcgt gcccgctttt ggccgctttc cctgtcagca gtaatccatg ccacatgctc
     1981 acggatgttc gcaatggcca ggaggtaagg atttaagccg gcttcctgaa gaacctttcg
     2041 aaatgtggcc tcgtgcaaat gaggcgaaca agcagccacc acgatgcgat tcaggccgta
     2101 ttccctaatg tcctggcgga tgagttcctg gccaggttca gaacacatgt atttgtaatc
     2161 ccgggcaacg tgcacatggg gcaagcctga cgcaaagcga gcaaggtcgg cggtatccag
     2221 gaccccggca atgttggtac cacaatggca cacgtaaaca ccaatgcgca gcgcatccgc
     2281 catcatgatc ccacctccgc tatcagtttt atttcagatg gcttgtaatt caaaaagcca
     2341 agatgaaagt ttcaatcttg acttttaatg tcaggagaaa tggcttgcgc tagcaaactg
     2401 gccacgtcaa tcacccggag tcgatcctct gcgcctagag atttcaccgc atcttgaaac
     2461 atgaccaggt cttttggaca tgccacgacc aggtcctcca cgttgcccag agaaagcgct
     2521 tccttgaccc tggtctcggc cggtcgctcc cgaattccag gacgatcctt catccagatg
     2581 cgcccaccac ctgcaccaca acagtagcta ttctctcggt gctg
//
LOCUS       DAOPTR010000556         2621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_31239_length_2621_cov_3.276695, whole genome shotgun sequence.
ACCESSION   DAOPTR010000556 DAOPTR010000000
VERSION     DAOPTR010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31239_length_2621_cov_3.276695"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PKI05_10615"
     CDS             <1..283
                     /locus_tag="PKI05_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP--guanido phosphotransferase"
                     /protein_id="HOA52696.1"
                     /translation="QRVHDRISRAYGVLKTAQIISSQETLECLSLVRMGVHLGLVKDI
                     SIPLLNELFLYTQPAHLQKVVGVEMDSADRNVERARFLKKKLGTAGPMG"
     gene            complement(335..1774)
                     /gene="purB"
                     /locus_tag="PKI05_10620"
     CDS             complement(335..1774)
                     /gene="purB"
                     /locus_tag="PKI05_10620"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOA52697.1"
                     /translation="MSKSDPYAMYDNPLVGRYASQEMSELFGPRRRIETWRRLWVILA
                     ESQAELGLPITEQQIAEMKSQISNIDWEKAAEYERRFRHDVMAHVHAFGDVCPSARGI
                     IHLGATSCYVTDNTDLILMREGLKLIARRLAAAIDHLGTFAAKYRDLPCLSFTHFQPA
                     QPTTVGRRACLWAYDFAMDLQEVEHRLSQLQARGVQGTTGTQASFLTLFNGDHEKVKK
                     LNQLVCQKMGFPGSYVITGQTYSRKIDAQILATLAQIAATAHKVATDIRLLAHRKEIE
                     EPFEKEQIGSSAMAYKRNPMRSERICGLARYVMSLEANTTLTHGVQWLERTLDDSANR
                     RLVIPQAFLAIDAILILCCNVFSGLVVYPKVIEKNLLAELPFMMTEDILMAAVQAGGD
                     RQELHERIRQHSHQVARKIKEEGGENDLIERLKADPAFAEVNFESVTDPLKYVGRSRE
                     QVDEFIRDVVQPIRERYTGARDLAAEVQV"
     gene            complement(1830..>2621)
                     /locus_tag="PKI05_10625"
     CDS             complement(1830..>2621)
                     /locus_tag="PKI05_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent phosphohydrolase"
                     /protein_id="HOA52698.1"
                     /translation="VDNMDFVLRDAYMSGYSQKAFDLERLIHYSFFTPQGVSIHERGL
                     SALVRFITVRAELFRSIYFHRTVRSIDLSLQDLFQESANLLFPGNPLDHLEEYRRLTD
                     WSLLVDVERWAESSDERRARLGHQWREWLARKIRWKMVCERTIFFGPGKAEEMSVFSD
                     EHLFEEAVRRRLPSDLRDMPLRFDPPRHVHRPGTRAPAAGQNFLYDPAQNAVRSLDAR
                     ELYRQIPLSYRICRVYALSTEHASEIAQAMDSLLSEDHVDDVTNM"
BASE COUNT          598 a          709 c          704 g          610 t
ORIGIN      
        1 ccagcgggtt cacgaccgga ttagtcgagc atatggtgtg ctaaagacgg cgcaaattat
       61 ttcctcgcaa gagaccctgg aatgcctgtc tctggtgcgg atgggcgtcc accttggttt
      121 ggtcaaggat atttccatcc cgcttctcaa tgagttgttc ttgtacaccc agccagccca
      181 tcttcaaaag gttgtcggtg tggagatgga cagcgctgac cgcaacgtag agcgggcacg
      241 gttcctgaag aagaaactcg gaaccgcggg gccaatgggg taaaggaccg cattggcagg
      301 agtctcaccc ttatcgatca caccatccct gtattcacac ctggacttcg gcggcaagat
      361 ctctcgcccc cgtataacgt tcccgaatgg gctggaccac gtctcggatg aattcgtcaa
      421 cctgttctcg ggaacgccct acgtatttca gtgggtcggt cacactttca aaattgacct
      481 cagcaaaggc tgggtcggcc ttaagacgct cgatgagatc gttttcaccc ccttcctctt
      541 tgatctttcg ggcaacctga tggctatgct gccgaattcg ctcgtgtagt tcctgccgat
      601 caccacccgc ttgcacggca gccatgagga tgtcttcagt catcatgaaa ggcaattctg
      661 ccaaaaggtt cttttcgata accttgggat aaacaaccag acccgagaag acgttgcagc
      721 agagaatgag gatggcatcg attgccaaaa acgcttgcgg gatgacgagg cggcgattgg
      781 cactatcgtc cagggttcgt tccaaccact ggacgccatg cgtgagggtg gtgttcgcct
      841 ccagactcat cacatatcgc gccaggccgc aaattcgctc actccgcatg gggtttctct
      901 tgtatgccat ggcggaagag ccaatctgtt ctttttcaaa cggttcttcg atctcttttc
      961 gatgtgccaa taggcgaata tcggtagcca ccttgtgagc agtggcggca atttgggcga
     1021 gggtcgcaag aatttgggca tcgatcttcc gagaataggt ttgtcccgtg atgacatagc
     1081 taccaggaaa tcccatcttc tggcaaacta attgattgag ctttttcacc ttctcgtgat
     1141 cgccgttgaa cagcgtaaga aaactggcct gcgttcccgt ggtcccctga acaccgcggg
     1201 cctgtaactg actgagtcgg tgttccacct cctgcaaatc catggcaaaa tcgtacgccc
     1261 aaagacaagc gcgccttccc accgtggtgg gctgtgccgg ttggaaatgg gtaaagctga
     1321 ggcagggcaa gtcccggtat ttggcggcaa acgttcccaa atgatcaatg gctgctgcca
     1381 gacgacgggc aatgagcttc aacccttcgc gcatgagaat gaggtcggtg ttatcggtga
     1441 cataacaact ggtggcaccc aagtgaataa ttccgcgggc ggaagggcaa acatcaccaa
     1501 aggcatggac gtgagccatc acgtcatgac gaaatcgccg ctcgtattcc gccgcttttt
     1561 cccaatcaat gttgctgatc tggcttttca tctcggcaat ttgctgttcg gtaatcggaa
     1621 gccccagctc ggcctgggat tcggccaaga tgacccacaa ccgtcgccag gtttcgatgc
     1681 gccgccgagg cccgaaaagt tcgctcattt cctgtgaggc atatcggccc acaaggggat
     1741 tatcgtacat ggcatatgga tcactttttg acatagcgga ttaccttctg cttggaggaa
     1801 ccgattctag cgacatgcat taccgagttt cacatattgg ttacgtcatc aacatggtct
     1861 tcgctcagta gggaatccat ggcctgtgca atctcggatg cgtgctctgt cgagagtgca
     1921 tagacacggc agatgcggta actgagcgga atctggcgat agagttccct ggcatcgagg
     1981 ctgcgaaccg cattttgggc cgggtcgtac aagaaattct gcccggcggc gggggcacgt
     2041 gttcccggac gatgaacatg ccgcggtggg tcaaagcgga gcggcatatc tcgcaaatcg
     2101 gaaggaagtc ggcgacgaac tgcttcctca aataggtgct catcgctgaa aacgctcatt
     2161 tcttctgctt tgcccggccc aaaaaagatg gttcgttcac agaccatttt ccagcggatt
     2221 ttgcgggcta accattcccg ccactggtgc cccaaacgcg cccgccgctc gtcggacgat
     2281 tcggcccagc gctcgacatc gaccaacagt gaccagtccg tcaaacgcct atattcttct
     2341 aggtgatcta aaggattgcc gggaaatagc aggttcgcac tctcctgaaa caagtcctga
     2401 agagacaggt cgatcgaccg gaccgtcctg tggaagtaga tggaccgaaa cagttccgcc
     2461 cgcactgtga tgaagcgcac cagtgccgac aatcctcgtt cgtggatcga cacaccttga
     2521 ggtgtgaaaa agctgtaatg gatgaggcgt tcgaggtcaa atgccttctg actgtatccc
     2581 gacatgtagg catctcgtag gacgaaatcc atgttatcga c
//
LOCUS       DAOPTR010000557         2618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_31279_length_2618_cov_2.720640, whole genome shotgun sequence.
ACCESSION   DAOPTR010000557 DAOPTR010000000
VERSION     DAOPTR010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31279_length_2618_cov_2.720640"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="PKI05_10630"
     CDS             complement(<1..642)
                     /locus_tag="PKI05_10630"
                     /inference="COORDINATES: protein motif:HMM:NF018484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HOA52699.1"
                     /translation="MASDNDTSPRPEVSLQPEEGWHCQHWFYRFDRMRLRTLAPGEYT
                     DGCKDFCETLDPRSPEAPLRLQTFVVMGHKADFGILMLDPDPLKLDRVHQRLLAGLLG
                     PTIEPMWSFVSMTEVSEYVPTVAHYEECLRQEGLVPDSPEYVQRTQQYARRYEIMRRQ
                     RLQPDLPNWPAFCFYPMNKRRLPEANWFLLDFEQRDRLMREHGETGLKFAGRVT"
     gene            1218..1721
                     /locus_tag="PKI05_10635"
     CDS             1218..1721
                     /locus_tag="PKI05_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOA52700.1"
                     /translation="MLNRHLADKILRLLTEERLSQRQVARLTGVSRATIAAIAKGEWH
                     ARFRERPKDESDQPPAPSRCPECGALVTPPCLACRIRAMRIRGEILPLPGEGPGPLKL
                     ELRGPHYARYLEVRAAAMARGEGEPLEDPTEDAADDDAWSDEAFWADESPSHNLPSPV
                     WRERGRG"
     gene            1904..>2618
                     /locus_tag="PKI05_10640"
     CDS             1904..>2618
                     /locus_tag="PKI05_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52701.1"
                     /translation="MTQFRYLVPLHQAQDWIRDGDLLLFRRRGLIAAAGRGEHSHAAL
                     AGWWGETLCCLEVRGRSGGRVVTLASQVEQFPGQIDVFLADPDNRWPEFDRRQTVQAM
                     LQFAGTRYGYRNFLRVALRHIPGIRLIMSPKTDDSAMDTLGPICSQACVMATRLGGGV
                     DPVPQLADELTEPADLARSPFYRYRLTLIPDDVTQPTPEPQPSSAEPPRWLRLLTLVG
                     VFLLIFVLTGRPIQGQSSCP"
BASE COUNT          545 a          844 c          707 g          522 t
ORIGIN      
        1 ggtcacccgt ccggcaaact tcaaacccgt ctcgccgtgc tcccgcatta ggcgatcgcg
       61 ctgctcaaaa tccagcagga accaattagc ctccggcaag cgacgcttgt tcatcggata
      121 aaaacaaaag gcaggccagt tgggtaaatc cggttgaagc cgctgccggc gcataatctc
      181 ataacgccgt gcatactgct gtgtgcgttg cacatattcg ggcgagtcgg gcactaggcc
      241 ctcttgacgc aagcattcct cgtagtgcgc taccgtggga acgtactctg agacctccgt
      301 catcgaaaca aacgaccaca tcggttcaat ggtggggccc agcaaacccg ccaatagccg
      361 ctgatgaaca cgatccaact tgagcggatc ggggtcgagc atcaagatcc cgaaatcggc
      421 cttatggccc atgacaacaa aggtctgaag tcgcaaaggg gcctccgggg aacgcggatc
      481 gagcgtctca caaaagtcct tgcacccgtc ggtgtactct ccgggggcca atgtacgaag
      541 tcgcatgcga tcaaaacggt aaaaccaatg ctgacagtgc caaccttcct caggttgcaa
      601 ggagacctct ggccgcggtg acgtatcgtt gtcagacgcc atgaaccaac ctctcgaacg
      661 tgtccaacca tcggtcaacc atcccgcccg ggaccccgca ccccaacaca cttcgcaaaa
      721 ggattacccg aaccaggaat tgttgcctcc cctgccccga aagacaagtc aacgactctc
      781 aagccggccg acctgcccca acgcaagctg gaagggaatg gccggccgcg ccgccgtgac
      841 ggtaaatctt gctcaatacc gaacgtcgca acatcaagaa acaccccgca ctccaaaatt
      901 gcccacccag cactcctttt gtgacatcaa tagtacatat tcatcacaat gtgattcact
      961 gtccttcttg aaaatagtcc acttcgtgtg ttatactgcc ctccgttgtg gctttgacaa
     1021 acttcgattg taagggcaga gcgtgggcag ctcgtccatt tcaggccctc cgaaaacccc
     1081 accgggacga gagccccttt gtgcgctgcc cggcaatgtt cacgacccaa gcgcggactg
     1141 acgtccgttt ttttggggcc cttgtagtgt tcatatgtac attaccaagc gacgcatgca
     1201 ccacgcggag gttacccgtg ctcaaccgtc acctcgccga taagattctc agactcctca
     1261 cggaagaaag acttagtcag cgccaggtgg cccgattgac aggcgtaagt cgtgccacca
     1321 ttgccgccat cgccaaggga gagtggcatg cccggtttcg ggagcgtccc aaggacgaaa
     1381 gtgatcaacc gcccgctccc agccgctgtc cggagtgcgg tgcccttgtt acaccgccgt
     1441 gcctggcttg ccgcatccgt gccatgagga tacggggtga gattcttcct ctgccgggcg
     1501 aaggtcccgg tcccctaaag ctcgaactcc gcggtccgca ctatgcccgc tatctagaag
     1561 tccgcgctgc cgccatggct cggggtgaag gagaacccct cgaagacccc accgaggatg
     1621 ccgccgatga tgacgcctgg tccgacgagg ccttttgggc agatgagagt ccatcccata
     1681 atctcccctc gcccgtttgg cgggagaggg gtcgggggtg agggtggctc gaattggtgc
     1741 cggttttctc aatcgattcc gacgttcgct tgccctcacc ccaaccctct cccgaaggga
     1801 gagggggact ttttgggata ggctcaaacc cagaagtgat gcctctgcgg ggcgacaggc
     1861 ccggtgactc tgttaccccc gatgttcaac ggagggccgc ctgatgaccc aatttcgtta
     1921 cctcgtgccg ctacaccaag cccaggactg gattcgcgac ggcgatttac tcctctttcg
     1981 gcgccgcggt ctgattgccg ctgcgggtcg gggggagcat tcccacgctg ctttggccgg
     2041 ttggtggggg gagaccctct gctgcctcga agtgcgggga cgttccggcg gtcgtgtcgt
     2101 cacgttggcc agccaagtcg aacaattccc agggcaaatc gatgtctttc tcgccgatcc
     2161 ggacaaccgc tggccagaat tcgaccgccg ccagacggtg caggccatgc tgcaattcgc
     2221 gggaacacgc tacggctacc ggaattttct ccgcgtggcc ctccgccaca tcccggggat
     2281 aagactcatc atgtcaccca aaacggatga ttccgccatg gacacgctgg gtcccatttg
     2341 cagccaagcc tgtgtaatgg ctacacgcct cggcggtggt gtggaccccg ttcctcaatt
     2401 ggccgacgaa ttgaccgaac cggccgacct cgcgcgcagc cccttctatc gttatcggct
     2461 cacgctcatc cctgatgatg tgacccaacc gaccccggag ccgcagccct cctccgccga
     2521 acccccgcga tggctacgac tgctgacact cgtcggtgtc tttcttctca tctttgtttt
     2581 gacgggaaga ccgatccagg gccaatcctc gtgtcccg
//
LOCUS       DAOPTR010000558         2616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_31301_length_2616_cov_3.664194, whole genome shotgun sequence.
ACCESSION   DAOPTR010000558 DAOPTR010000000
VERSION     DAOPTR010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31301_length_2616_cov_3.664194"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2472
                     /gene="dnaE"
                     /locus_tag="PKI05_10645"
     CDS             <1..2472
                     /gene="dnaE"
                     /locus_tag="PKI05_10645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOA52702.1"
                     /translation="GSGVGSLVSYALKLSHVCPIEYGLLFERFLDLNRREAPDIDIDF
                     CQERREEVLQYVKEKYGHNNVAQICTFGTMAARAAIRDVGRVLGHPVAFVNEITALVP
                     EGPKVTIQAALEKSEDLRKRYETDLRVREVIDYAKQIEGLARNVGTHAAGVVIADKPL
                     VEYVPLQHVPGKEEVITQWAMADVERAGLLKMDFLGLRNLTILSKVIGLIEQTTGEKV
                     DPYSFPLDDTKTYELLCRGETKGVFQLESGGIRELLQRMKPDNIRDIIAVNALYRPGP
                     LEGGMVDDYIDVKHGRKKPEYKHPVMKEILEETHGVMVYQEQVMQILNRLGGIELADA
                     YTCIKAISKKKLDKIAKYREQFVKGACEKGLTQKESEELFTLIEKFAGYGFNKSHSTA
                     YARIAYITAYLKAHYPLQFMAALLSSDIVNRNFTRKDSLVEHLEDCHRMGIEVIPPDI
                     NLSEVEFTVKDGKIVFGLAAIKGCGGSAAEAIVEERRRHGPFRTLFEFCERLEQGSVN
                     RSAIESLIKAGAFDKLGGHRAQLLAVLERAIQAGASAAEDRRRGQLSLFGNNSTRNPQ
                     VVDQQLPDIPEWPDHERLAKEKEVLGFYFSSHPLREYEELLGRYCTHTTADLGQLAHG
                     TEVVVGGVVSAIVTKHTRAPRNDGPTKFAMFDLEDTAGIVRCILWPEPYTRFGEYLHD
                     DAVLVALATVDKRGGGEEATLIIQELVPIAELKRRFTRGIQIRFSEAQHNEVTLQNLY
                     QIVRDHPGSCQLQLMLQLRDGRRVVMACDGMGVTLDTDLEQQIEKLLGQQAICYLAGR
                     FSLRNGNGNGNGHANGKNGSMRRSR"
BASE COUNT          615 a          710 c          750 g          541 t
ORIGIN      
        1 gggtcgggtg tcggatcgct cgttagctac gcgctcaaat tgagtcacgt ttgccccatt
       61 gaatacggcc tgctctttga acggttcctg gatctcaatc ggcgtgaggc gcccgatatc
      121 gacatcgact tctgtcaaga acgccgtgag gaagttctcc agtatgtgaa ggaaaaatac
      181 ggccacaaca acgtcgcaca gatctgcacg tttggtacga tggcggcacg ggcggcgatc
      241 cgcgatgtgg ggcgggtact cggacatcct gtggcattcg tcaatgaaat cacagccctg
      301 gtccctgaag gccccaaggt caccatccag gcagccctgg aaaaaagcga ggaccttcgc
      361 aaacggtacg aaacagactt gcgggttcgc gaggtcatcg actacgccaa gcagattgag
      421 ggtctcgccc gcaatgtggg cacccatgcc gcgggcgtgg tgatcgctga taaaccactg
      481 gttgaatacg tcccccttca gcatgtgccc ggcaaagaag aagtgatcac gcaatgggcg
      541 atggccgacg tggagcgggc aggtttgctg aagatggact tcttgggatt gcggaacctg
      601 accatcctct ccaaggtcat cggcttgatc gagcaaacca cgggcgaaaa ggtcgatccg
      661 tattcgtttc cgctggatga taccaagacc tacgaactcc tctgccgggg cgagaccaag
      721 ggcgtctttc agttggaaag cggtggcatt cgggagctgc tccagcggat gaagcccgat
      781 aacatccgcg acatcatcgc cgttaatgcc ctttaccggc cggggccgct ggagggcgga
      841 atggtcgatg actacatcga cgtcaagcat ggccgcaaga aacccgagta caaacacccc
      901 gtcatgaagg agattctgga agaaacccac ggggtgatgg tctatcaaga gcaggtcatg
      961 cagattctca atcgccttgg cggcatcgag ctggccgacg cctatacgtg catcaaggcc
     1021 atcagcaaga agaagctcga taaaattgcc aagtatcggg aacaattcgt caagggtgct
     1081 tgcgaaaaag gcctcaccca aaaggaatcc gaagaactat tcacgctcat cgagaagttt
     1141 gccggttatg gcttcaacaa gagccattct acggcctatg cccgaattgc ctacatcacg
     1201 gcttacctca aagcccacta tccgctgcag tttatggcgg cactgttgtc aagcgacatt
     1261 gtcaaccgca atttcacccg aaaggactcg ctggttgagc atttggagga ttgccaccgg
     1321 atgggtatcg aggtgattcc gcccgatatt aacctgtccg aagtggaatt caccgttaag
     1381 gacggcaaga tcgttttcgg tctggcggcg atcaaggggt gtggcggctc ggccgccgag
     1441 gccattgtgg aggagcgccg tcgccacgga ccattccgaa cgttgtttga gttttgcgaa
     1501 cggctcgagc aaggaagcgt taaccgctcg gctatcgaat cgctgatcaa ggccggggct
     1561 tttgataaat tgggcggtca cagggcacaa ctattggcgg ttttggaacg cgccattcaa
     1621 gcaggggcaa gtgctgccga agatcgtcgc cgtggacaac tgagtctctt tggtaacaac
     1681 agcacaagaa acccccaggt ggtcgatcag caacttcccg acatcccaga atggcctgat
     1741 cacgaaagac tggccaaaga gaaggaggtt ctcggctttt atttctcaag tcatcccctc
     1801 cgagaatatg aagaacttct tggccgttat tgcacgcata ccacggccga cttaggacaa
     1861 ctcgcccatg ggaccgaagt ggtcgttggc ggtgtggtgt cggccattgt taccaaacat
     1921 acacgggcac cacggaacga tggccccacc aagtttgcca tgtttgattt ggaagatacc
     1981 gcggggatcg tccgttgtat tctttggccg gaaccgtata cccgctttgg cgaatacctc
     2041 cacgacgacg ccgttctcgt tgctttggcc accgtggata aacgtggcgg gggtgaggag
     2101 gcgacgttga ttatccagga actggttccc atcgcggagt tgaagcggcg ttttacgcgg
     2161 gggatccaga tccgattttc ggaagcccaa cacaacgagg tgacactcca gaacctctac
     2221 caaatcgtgc gcgatcatcc aggctcatgt cagttgcaac tcatgcttca gttgcgcgat
     2281 ggtcgccgcg tcgtcatggc ctgtgacggc atgggcgtta ccctcgacac cgacctagaa
     2341 cagcaaatcg agaaactgct gggacagcag gccatttgct atctcgccgg gcggttttcg
     2401 ctccgcaacg gtaatggaaa tggcaatggc catgccaacg gtaaaaatgg gagcatgcgg
     2461 cgctctcgct gagagaagac atcatccacc gaaacgcggc cccatgacca gccttgctgg
     2521 atcgcccggc gggggttact caccaaccac ataaacgatc agtcgtttgg gaccgtgcac
     2581 gcccaagatt aagattttct cgatgtcggc ggtccg
//
LOCUS       DAOPTR010000559         2615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_31322_length_2615_cov_3.417969, whole genome shotgun sequence.
ACCESSION   DAOPTR010000559 DAOPTR010000000
VERSION     DAOPTR010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31322_length_2615_cov_3.417969"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(296..1552)
                     /locus_tag="PKI05_10650"
     CDS             complement(296..1552)
                     /locus_tag="PKI05_10650"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HOA52703.1"
                     /translation="MSASDRQTTPADFPLEQPRLAWFFGVLLAVVVWSYWNSLSEAAI
                     AWRGAQYSHGLFVPLIATFLLWVRRLGGTFDWQWQTTTEKWRLVAVGGVLALAWFSYI
                     ASERLGQQAAPGEAVSLTAASGLGFMAAMLYATGLLTWATFAADSWLQHSLESEKQRQ
                     NDEDKTHLRGELAGEALASGTQTALVRGRYVGLFILIVAVALRLASAYVGLDVAEMWT
                     LPLALLGAGVCTIGWRSLRWSWPAVVVLFFAFPLPFGVERTILVPLQGIAAQGGAYLL
                     QSLGLEASTEGAHFIRLGEFQLGVVEQCSGLRMTTVFITLVFVYVVVVPTPKWQIPVL
                     LLSSIPIALAVNILRITVTGTLYVYGSRELAQHVFHDWAGYLMPLAAVALLLLLRVLL
                     DNLFIKVEEQDVPLAQRLEPAKKGST"
     gene            complement(1802..>2615)
                     /locus_tag="PKI05_10655"
     CDS             complement(1802..>2615)
                     /locus_tag="PKI05_10655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOA52704.1"
                     /translation="PPENRETLVNTHAFLEGRIYIQDLSSMLAVHVLDPQPGEEILDL
                     AAAPGGKTCHVAARMKNQGRIAAVEVIPQRLYKLVANLERSGVTIVKTYLADGRTIGK
                     KTPNRFDRVLLDAPCSSEARFDARDPASWKHWNLRKIAECARKQKGLLLSALEAVRPG
                     GLVLYCTCSFAPEENEAVVDHVLQQAQGGVFVEALNIPLPNAIPGLTKWQGREYHPSL
                     ELACRILPTHEMHGFFMCCLRKTEEATDTYRRRERKWSRSPKHRPREKDAPS"
BASE COUNT          619 a          771 c          695 g          530 t
ORIGIN      
        1 caactcaagg agcctctccg ttttcctttc taattcctga gaaaggccag cgaaggctat
       61 ctgtggtgat tccatggagc tttccatcga aataagcgat aacttttcct ataaaccgta
      121 tttgacctcg atgatccctc cccaaaacaa gcggagtatc atattgtcga caaccgacag
      181 ataattgtca accaccgttg acattattgc ctttttcccc acaagcccca ttatacccca
      241 ctggcacgtg gttcggatga gcagcagagg ctattaacca ggaaggagac aaagtctacg
      301 tgctaccctt ctttgccggt tcaaggcgct gggcaagggg cacgtcttgt tcttccactt
      361 tgataaacag gttatctagc agaacacgaa ggagcagcaa caaggcaacc gcagccagcg
      421 gcattagata tccggcccaa tcgtggaaga cgtgctgggc caattcccga ctgccgtaaa
      481 cgtagagagt tcccgtcacc gtaatgcgga ggatgttcac agccaaggcg atagggatgg
      541 agctcaacag gaggacagga atttgccact ttggcgtggg caccaccacg acataaacga
      601 acaccaacgt gatgaagacc gtggtcatcc gcaagccact acactgttcc accactccca
      661 gttgaaattc acccagccga atgaaatgtg ccccctccgt ggatgcttct agtcccaaac
      721 tttgtaatag atacgcaccg ccttgggcgg ctattccctg gaggggaacg aggatcgtac
      781 gctccacacc gaacggcaaa ggaaaggcaa aaaacagaac gaccactgcc ggccagctcc
      841 agcggagcga cctccacccg atggtgcaaa cacctgcgcc caaaagagca agcggcaacg
      901 tccacatttc ggccacatca aggccgacat aggcggatgc cagtcgcaac gccacagcga
      961 caatcaggat aaacaatcct acgtagcgac ctcgcaccag ggcggtttgg gtcccgctgg
     1021 caagagcttc ccctgctaac tcccctcgaa gatgagtctt atcctcatcg ttttgccgct
     1081 gtttttcgct ttcaagcgag tgctgaagcc agctatccgc cgcaaaagtg gcccaggtga
     1141 gcaagcccgt ggcatacagc atggccgcca tgaaccccaa gcccgacgcg gcggtcagcg
     1201 aaacggcctc cccgggcgca gcttgttggc ccagcctctc cgaggcgatg tagctaaacc
     1261 atgccagggc gagcacaccc cccactgcaa caagacgcca tttctccgtg gtcgtttgcc
     1321 attgccagtc gaaggtcccc ccgagtcttc ttacccagag caaaaaggta gcaatcaggg
     1381 gaacgaaaag accatgagaa tactgggccc cccgccatgc gatggcggct tcactcaggg
     1441 aattccagta gctccagacc acaaccgcca ggaggacgcc aaagaaccaa gcgagcctcg
     1501 gctgttctaa ggggaagtcg gcaggagtgg tctgtctgtc cgatgctgac atggcttgct
     1561 taggttgccg ttctttccag tttacggatc ggtgacgtcc agcgagttgg agtagatcgc
     1621 taactcttta cggtaattca gcctccctgg ccttggcaag catctgtcgg tttttggctc
     1681 gggcaaaacg tccgacactc aaccgtgtga ctttcctcca ggctgacggc aagatagtcg
     1741 atgccaaaac ccgcggcgac ttcatgtcat ttgtgcccag cgggaccatg cgcagctagg
     1801 atcacgaggg ggcatctttt tcgcggggac ggtgcttggg agaacgcgac catttgcgtt
     1861 cgcgtcggcg ataagtgtcc gtggcttctt ccgttttccg gagacaacac ataaagaagc
     1921 catgcatttc gtgcgtggga aggattcgac aagccaattc cagcgaggga tggtattcac
     1981 gtccctgcca ctttgttaaa ccggggatcg cattcggcaa cggaatattc agcgcttcga
     2041 caaagacacc gccctgcgcc tgttgaagaa cgtgatccac aacagcctcg ttctcctccg
     2101 gcgcaaagga acacgtgcaa tagaggacaa gtccgcccgg ccgaacggcc tccagcgccg
     2161 aaagcagcaa tcccttttgc ttccgagcac attcggcgat cttgcgcagg ttccagtgtt
     2221 tccaactggc cggatcgcgg gcatcaaagc gggcctcgga cgagcaaggc gcgtcaagta
     2281 agactctgtc gaagcggtta ggggtcttct tgccgatcgt gcggccatcc gccaaatagg
     2341 ttttgacaat cgtgacgccg gaccgttcca ggttcgccac aagtttgtag aggcgttgag
     2401 gtatgacttc cacggccgca atacggcctt gattcttcat ccgggcggct acatgacagg
     2461 ttttcccgcc gggggccgcc gccaggtcga ggatctcttc gccgggttgc gggtccaaaa
     2521 cgtggaccgc cagcatgctc gacaaatcct gaatatagat ccgcccctcg agaaacgcat
     2581 gagtgttaac cagagtctct cggttttccg gcggg
//
LOCUS       DAOPTR010000560         2606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_31459_length_2606_cov_2.932183, whole genome shotgun sequence.
ACCESSION   DAOPTR010000560 DAOPTR010000000
VERSION     DAOPTR010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31459_length_2606_cov_2.932183"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1452)
                     /locus_tag="PKI05_10660"
     CDS             complement(7..1452)
                     /locus_tag="PKI05_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HOA52705.1"
                     /translation="MQINEALVKTVVEEVLHRLKGNGTPAAPPPVVKRFQGRLGLFTD
                     VDDAVAAAVEAYDMLSRRTLEERKRIIDHIRRIAIEQCVELGTMEMEETKIGRLEHKI
                     EKLRILGERTPGVEFLRSEVFSGDHGLTVIEHAPFGVIGAVTPVTHSLPTLTGNAINM
                     IAAGNTVVFNPHPSGKRVAAEGVRRYNSAIYRDLGIDNLICVITEPTIETANAIFKHR
                     DVALLCVTGGPGVVRVAMQQAKRAIVAGPGNPPVVVDETADLDRAARCIIQGAAYDNN
                     LLCIAEKEVLAVDKIFDQLMAAMERAGAVRLNASEIEALTAKAITYAGEGEHRHPVGV
                     KEFIGQDAAVLARAIGKKIPEDVELLFGETDFSNPFVHAEQMMPFVPFVRCRDVDEAI
                     EKARIAEHGFRHTAIIHSHDVVNMTKMGRALETTIFVKNGPSMAGLGLGGEGYLSYSI
                     AGPTGEGITTPLTFTRERRCAMIDSLRILGK"
     gene            complement(1609..1944)
                     /locus_tag="PKI05_10665"
     CDS             complement(1609..1944)
                     /locus_tag="PKI05_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOA52706.1"
                     /translation="MFLAKVTGSLVSTQKVDSMVGRKLLLVEPYRVDPQTRSQLVGTG
                     RTFVAVDTVGAGEGELVLIVQGSSARLTPETKNLPVDTVIIGIVDHVHVEQRSVYSKQ
                     PEDRPKNSV"
     gene            complement(1947..>2606)
                     /locus_tag="PKI05_10670"
     CDS             complement(1947..>2606)
                     /locus_tag="PKI05_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HOA52707.1"
                     /translation="HGASHRYIAVRTAQLLGREDLRIISCHLGGSSSVCAIRNGQSVF
                     TSMGMSPQSGLPNNNRVGDFDPFALPVIMKRTGMSLSQVLSVLANESGLLGLSGVSTD
                     LRDILQAANQGNWRAQLAVDVFVASIRKYLGGSLVELGGADVLVFTGGIGENQPVIRT
                     AVCRGLTELGIVLDETKNQQAQGEAEIGHETSRTRIWVIPTNEELIVARQAKALLESG
                     Q"
BASE COUNT          567 a          819 c          664 g          556 t
ORIGIN      
        1 acaacatcac ttgccgagga tgcggagact gtcgatcatg gcacagcgac gttcacgggt
       61 gaatgtcaac ggcgttgtaa tcccctcacc tgttggccca gcaatggaat acgaaaggta
      121 gccttcacca ccaagcccca gtcctgccat ggacgggcca tttttcacaa agatggttgt
      181 ctctagtgcc cgtcccatct tcgtcatatt caccacgtca tgggagtgaa taattgccgt
      241 gtgacggaag ccatgttcag caatccgcgc cttttcgatc gcttcatcga catcccgaca
      301 gcggacaaat ggcacgaagg gcatcatttg ttcggcatgg acaaagggat tcgaaaaatc
      361 ggtttccccg aagaggagct ccacatcttc agggatcttt ttgccaatcg cccgggccaa
      421 cacggccgca tcctgcccaa taaactcctt caccccaacg ggatggcgat gttcaccctc
      481 cccggcgtac gtgatggcct tggccgtcag agcctcgatc tctgaggcgt tcaaacgaac
      541 agcgcctgcc cgttccatgg ccgccataag ttgatcgaag atcttgtcca cggcgaggac
      601 ttccttttcg gcgatgcaga ggaggttatt gtcgtaagcg gcgccttgaa tgatgcaacg
      661 tgccgcccga tcgagatcgg cagtctcgtc aaccaccacg gggggatttc ccggtccggc
      721 cacgatggcc cgttttgcct gctgcatcgc cacacggaca accccaggac cgcctgtcac
      781 gcacaagagg gccacatcac gatgtttgaa aatggcgttg gccgtctcga tcgtgggctc
      841 cgtgatgacg cagatcaagt tatctattcc caaatcacgg taaatggcgg agttgtatcg
      901 acgaacaccc tccgcagcca cccgcttgcc gctgggatgg ggattgaaca ccacggtgtt
      961 tcccgccgca atcatgttga tggcattgcc cgttaaggtg ggcaacgaat gcgtcaccgg
     1021 cgtgaccgca ccaataacgc cgaagggcgc atgttcgatg accgtcaagc catgatcgcc
     1081 gctgaatacc tcactccgca agaactcgac gcccggcgtc ctttccccga ggatgcgtaa
     1141 cttctcgatc ttgtgctcga gtcggccgat cttggtctcc tccatctcca tcgtgcccaa
     1201 ctcgacgcac tgttcgatgg caattcgccg gatgtggtcg atgatgcgtt ttcgttcttc
     1261 cagcgtccgc cgcgaaagca tgtcataggc ctctaccgcg gcagccaccg cgtcgtcaac
     1321 atcggtaaat agccccagac ggccttgaaa tcgcttgaca acaggtggcg gtgcggccgg
     1381 ggttccgtta ccctttagcc gatgcaaaac ttcctccacc accgttttca caagagcctc
     1441 attgatttgc atggtcacgc tccttatgga tgtcgtcgcg tgggaatcct cgcacacttc
     1501 ccgacgtcat caaaacttaa gaattcaccc tcgcaatcat acgtcatcac cacgcacatg
     1561 catccgttca tcacacggcc ccggccatcc acaactgctt cgccaagctt ataccgagtt
     1621 ctttggccgg tcctccggct gtttgctata gacgctccgt tgctccacgt ggacgtgatc
     1681 cacaatgcca atgatcacgg tgtccacggg aaggtttttg gtttcgggag ttaagcgggc
     1741 gctcgatccc tgaacgatca gcacaagctc tccctcgccg gccccgacgg tatcgacagc
     1801 cacaaacgtg cggcccgttc ccaccaactg agatcgcgtc tgtggatcaa cacgataggg
     1861 ctcgaccagg aggagcttac gccccaccat ggaatcgacc ttctgggtgg aaaccaacga
     1921 acccgtcact tttgcgagaa acatatttat tgccctgact ccaaaagggc cttggcttgt
     1981 cgagccacaa tcaattcctc attggtggga atcacccaaa tccgggtgcg actcgtctcg
     2041 tgaccgatct cggcctctcc ctgtgcctgc tgattcttcg tctcatcaag cacgatgcca
     2101 agctcggtta aaccgcggca aacggctgtc cggataaccg gttggttctc gccaatcccg
     2161 ccggtgaaga ccaggacgtc tgcaccgccc aattcgacca gtgaaccgcc gaggtacttg
     2221 cggatcgacg ccacgaacac atcgaccgcc agttgcgcac gccagttccc ctggtttgcc
     2281 gcctgaagga tatcgcggag atcggtactg acgccactca aaccgagcaa accgctctca
     2341 ttcgccagca cagaaagcac ctgtgatagc gacatacccg tccgcttcat aatcacgggc
     2401 aaggcgaacg ggtcaaagtc gcctacgcga ttattattgg gcaaccccga ttgggggctc
     2461 attcccatgc tggtgaaaac gctttgccca ttgcgaatgg cgcacactga actggaccca
     2521 ccgagatggc aggaaatgat ccgtaggtcc tcacggccta acaactgcgc tgtccgaacg
     2581 gcaatgtagc ggtggctagc cccatg
//
LOCUS       DAOPTR010000561         2605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_31469_length_2605_cov_3.343529, whole genome shotgun sequence.
ACCESSION   DAOPTR010000561 DAOPTR010000000
VERSION     DAOPTR010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31469_length_2605_cov_3.343529"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2572)
                     /gene="nifJ"
                     /locus_tag="PKI05_10675"
     CDS             complement(<1..2572)
                     /gene="nifJ"
                     /locus_tag="PKI05_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOA52708.1"
                     /translation="MAFSEGFHPHPKLSFGPALAVGISSAAEYFDMELVRAHRERAMS
                     PEKPVLRGTAQNPDVFFQAREACNPFYDKTPAIVQKAMDKFAKLFGRQYHLFDYVGAP
                     DADRVVVIMASGTGVVEEAVDYLVERGEKVGMVKVRLYRPFDADALVRVLPKTVKRIA
                     VMDRTKEPGALGEPLFLDVVAAINERWRDHALGTELPLIIGGRYGLSSKEFNPAMALA
                     VFEELKKPQPKRRITVGINDDVTHLSLSYDPTFSIERPDVVRALFYGLGSDGTVSANR
                     SSVKIIGENTPFYAQGYFVYDSRKAGSMTISHVRFSPRPIKGSYLINKANFIACHQFH
                     FLERIDMLSAAEEGATFLLNSPFPAEEVWDHLPAEVQKQIIDKKLKFYVVDAYRVAKE
                     TGLGVRINTVMQTCFFALANVIPVEQAIKEIKDQIVKSYGKKGGSAIIEKNFAAVDKA
                     LESLREVKVPDRVTSNIHMVPPVPEDAPPFVKNVLGPMIAYRGDDLPVSALPVDGTFP
                     TGTTKYEKRSIALEIPIWDPKICIQCGLCSLVCPHAAIRMKAYDPAVLANAPEGFKSA
                     PWRGKEYPGYHVTVQVAPDDCTGCGLCVEVCPVRDKEVAKHKAIDMCFKLDHLDRERA
                     CFDFFLKIPDLDRSKVAVDTVKGSQLLLPLFEFSGACAGCGETPYVKLITQFFGDRMM
                     IANATGCSSIYGGNLPCTPYTTNAEGKGPTWNNSLFEDAAEFGFGMRLAVDQQIEYAT
                     GLLKKLAPQIGDTLVTELLNNVQETDEQIAKQRQLVAELKKKLATIPGDDAANLLASA
                     DYLIRRSIWSFGGDGWAYDIGFGGLDHVLASGRDINLLVLDTEVYSNTGGQASKATFR
                     G"
BASE COUNT          590 a          733 c          696 g          586 t
ORIGIN      
        1 ccccgcggaa ggtggctttc gacgcctggc cgcccgtgtt ggaataaacc tcggtatcta
       61 agacaagcag gttaatgtcc cgcccggacg ctaagacgtg gtctaatcca ccaaaaccga
      121 tatcataggc ccaaccatcc ccaccaaagc tccaaatact tcggcggatg agatagtcag
      181 cggaggcgag gagattcgcg gcgtcatctc cagggatggt tgccagtttc ttcttcaatt
      241 cggcgacgag ctggcgttgc ttcgcgatct gctcatcagt ttcctggaca ttattgagca
      301 actcggtaac aagcgtatcc ccaatctgcg gggccaattt cttgagaagt cctgtggcgt
      361 attcgatttg ctggtccaca gccaatcgca ttccgaagcc aaattcggct gcgtcttcaa
      421 agagagagtt gttccaggtg gggcctttgc cctcggcatt ggttgtgtac ggggtgcacg
      481 gcaagtttcc accataaatc gacgaacagc cagtggcgtt ggcgatcatc atgcgatcgc
      541 cgaagaattg cgtgatcagt ttgacatacg gtgtctcgcc gcaaccggcg caagctcctg
      601 agaactcgaa caacgggagt aagagctgcg accccttgac cgtatcgacg gccaccttgg
      661 aacgatcgag atccggaatc ttgaggaaga aatcgaaaca agctcgttcg cgatcgagat
      721 ggtccagctt aaagcacata tcgatggcct tgtgcttggc cacttctttg tcgcgaaccg
      781 gacacacctc gacacacaat ccacagccgg tgcaatcgtc cggtgccacc tgaacagtca
      841 cgtgatagcc cggatactct ttgcctcgcc acggtgcgga cttgaatcct tccggtgcgt
      901 tggccagaac ggcgggatca tatgccttca tgcgaatagc cgcatgtggg caaacgaggg
      961 aacacaaacc acactggatg caaatcttgg ggtcccaaat gggaatttcc agggcaattg
     1021 aacgtttttc gtactttgtg gtgcctgtcg ggaaggtgcc atcaacgggc agcgcgctga
     1081 caggcaaatc gtccccacgg taagcaatca tcggccccag aacgttcttc acaaatggtg
     1141 gcgcatcttc gggcaccggc gggaccatgt ggatattgga cgtgacccga tctggtactt
     1201 tcacttcccg cagtgattca agggccttgt caacggcggc aaagttcttt tcgataattg
     1261 ccgacccgcc ctttttgccg taggacttga cgatctgatc cttgatctct ttgatggcct
     1321 gttcgactgg aatgacgttg gccaaggcga agaaacacgt ctgcatcacg gtgttgatgc
     1381 gcacacccag gcccgtctcc ttcgccacac gataagcatc tacgacgtaa aactttagtt
     1441 tcttgtcgat aatctgcttt tgtacctctg ccgggagatg gtcccaaacc tcttctgcgg
     1501 ggaacgggga attgagcagg aatgtggccc cttcctccgc ggccgacagc atgtcgattc
     1561 gttccaggaa atgaaattga tggcacgcga taaagttggc cttgttgatc aggtaggacc
     1621 ccttaatcgg ccgtgggcta aagcgcacgt gggaaatcgt catcgacccg gctttccggg
     1681 agtcgtaaac gaaataccct tgggcgtaaa acggagtgtt ctcgccaatg attttcacag
     1741 aactacgatt tgcagaaacg gtcccatcgc ttcccagccc gtagaataac gcccggacca
     1801 catcgggccg ttctatggag aaggttgggt catagctcaa gcttagatgg gtgacatcgt
     1861 cattaatgcc aacggtgatg cgccgcttgg gctgaggctt tttcaattcc tcaaacacgg
     1921 ccagggccat cgcaggattg aactccttcg acgagaggcc ataccgacca ccaataatca
     1981 gcggcaactc ggttcccagg gcgtgatccc gccaccgttc gttaattgcg gccacgacgt
     2041 ccaagaatag tggctcaccc aaggcacccg gctccttggt tcggtccatc acagcgatcc
     2101 gtttgacagt tttaggaagc actcggacga gtgcgtcggc atcgaacggg cgataaaggc
     2161 gaactttcac catgcccacc ttttccccgc gttccactaa gtaatcaaca gcttcctcca
     2221 ccaccccggt tccagaagcc ataatgacga cgacccggtc tgcgtccggc gccccgacat
     2281 aatcaaagag gtgatactgt cggccaaaga gcttggcgaa cttgtccatt gccttttgga
     2341 cgatcgccgg cgtcttgtca taaaagggat tacaggcctc tcttgcctgg aaaaagacgt
     2401 ctgggttttg agccgtaccc ctgaggacgg gcttttcggg cgacattgct cgttcgcggt
     2461 gggcccggac caattccatg tcgaagtatt cggcggcgct gcttatcccc accgccaaag
     2521 cgggaccgaa actaagtttg gggtgaggat gaaatccctc ggaaaacgcc attggtaatc
     2581 cggctctccg gatcgcccgt tcaat
//
LOCUS       DAOPTR010000562         2605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_31473_length_2605_cov_3.204314, whole genome shotgun sequence.
ACCESSION   DAOPTR010000562 DAOPTR010000000
VERSION     DAOPTR010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31473_length_2605_cov_3.204314"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            629..>2605
                     /gene="ppdK"
                     /locus_tag="PKI05_10680"
     CDS             629..>2605
                     /gene="ppdK"
                     /locus_tag="PKI05_10680"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOA52709.1"
                     /translation="MSQAAKYVYYFGPEGTEGNASMVNLLGGKGANLAEMAGHPDLRL
                     PVPAGFTITTEVCTYFMQHGGKYPPGLEKQVEEALRKVEKAMGMKFGDPENPLLVSCR
                     SGARRSMPGMMETVLNVGLCTKTIPGLIKKTNNPRFVYDAYRRLIMMYSDVVMEKAEG
                     IEPPEGMGIRVQLERIMEKLKHEKGYEKDTDLTAEDLQKLCEEFKAKVKEVLGKPFPD
                     DPYEQLWGGISAVFKSWNGKRAVSYRRIEGIPDEWGTACNVQSMVFGNMGDTSATGVA
                     FTRNPATGENVFYGEWLPNAQGEDVVAGIRTPNPLNKATKTEQNRHLPSLEEAMPELY
                     RQLDEIRVRLEKHYKDMQDIEFTIQEGKLYMLQCRVGKRTGTAALNMAMDMLHEGLID
                     EETAVLRVAPKQLDELLHPVVDPNAEKKAKPIAAGLPAGPGGACGQIVFTSEDAVEWA
                     KKGKKVILVREETNPEDVEGMRAAEGILTARGGMTSHAALVARGWGKCCIVGAGKIKV
                     DVEKRCMTIDGKVLKEGDFVTLNGTRGLVYEGKLGLIDATENPRLKEFMALVEKYRKL
                     GVRTNADTPADAKVARDFGAEGIGLFRTEHMFYGEGSDEPLFILRKMILSRTAEEHRK
                     ALDELFPFLKRDIKSTLEAMDGLPVTIRLLDPPLH"
BASE COUNT          616 a          654 c          814 g          521 t
ORIGIN      
        1 ccgctagatt ccactgaatg tgatcccgca gcgggatgga gaaaagccac gcaagggcta
       61 agccgatcaa tcccaaaacg cattcggcca gcgtcccgat gagaacgaac tgccgtgaaa
      121 gttgatgttt acgccgatcg aaagctgcgg gtggttctgg cgacataata ctgaaaggcg
      181 gttcggggat tcgggactca ggctaaacaa agtcattcag ccccatgtag cccctttgaa
      241 caaagttctg agcgcaatcg taagaaaaac tcaattttcg gatttcctga ggcataggtc
      301 cagtgtcatt tgcagtaaaa gggatttgtc aatccggacc atcagcgtca tcgggaaagc
      361 cgggaagaat ctgtgtttcc aaaaggatgc gcgtcattgt gaattcacct atccttgtaa
      421 tggacattga cccatcggac ggggttcgtt gaccctctcc tggcctaggg ttaaactaca
      481 ggattctatt ttggaaagtg caccagtgtc ctgcgtcgcg tcaacgagcg ctgtgcggtg
      541 gtagtccaga ctacccgaaa ggggttgcgg tcgtgggggt gcaagcggtt tggttgtttg
      601 tgcggtaagt cacttaggag attgaacaat gtcacaagcg gcaaagtacg tctattattt
      661 cggtccggag ggaacggaag gtaacgcctc gatggtcaac ctccttggtg gcaagggggc
      721 gaatttggcg gagatggcgg ggcatcctga cctacggctc cctgtcccgg ctgggttcac
      781 gattaccacg gaagtatgta cttacttcat gcaacatggt gggaaatatc cgccgggatt
      841 ggaaaagcag gtagaggaag ccctcaggaa agtcgaaaag gcgatgggta tgaagttcgg
      901 ggacccggag aatccgttgc tggtctcctg ccgttcgggt gcccgccgtt ctatgccggg
      961 catgatggaa acagttctca acgtgggcct ctgcaccaag acgattccgg ggttgatcaa
     1021 gaagacgaat aatcctcggt tcgtgtacga cgcttaccgc cggctcatta tgatgtactc
     1081 cgacgtcgtc atggaaaagg ccgaggggat cgagcccccg gaagggatgg gcatccgggt
     1141 ccagctcgag cgaatcatgg aaaagcttaa gcacgagaaa ggctacgaga aggacacgga
     1201 tttgaccgcc gaggatttgc agaaactctg cgaagagttc aaggcgaagg tcaaggaagt
     1261 gctcggaaag ccattccccg atgatcccta tgaacagctt tggggaggta tttccgcggt
     1321 ctttaagagc tggaacggca agcgggccgt ttcctaccgt cgtattgagg gtatccccga
     1381 cgaatgggga actgcctgca acgtgcagag catggtcttc gggaacatgg gcgacacctc
     1441 ggccacggga gtggctttta cacgaaatcc ggcgaccggc gagaacgtgt tctacggcga
     1501 atggttgccg aacgcccagg gcgaggacgt ggtggcgggt atccgcacgc ccaatccact
     1561 caacaaggcg accaagaccg agcagaaccg gcacctcccc tccctggagg aagccatgcc
     1621 ggagttgtat cgccagttgg acgagatccg ggtgcggctt gagaagcact acaaggacat
     1681 gcaggacatt gagttcacca tccaagaggg caagctctac atgctccaat gccgtgtcgg
     1741 taagcgaaca gggacggctg ccctcaacat ggccatggac atgcttcacg agggcttgat
     1801 cgacgaagag acggccgttc tccgtgtcgc ccccaagcag cttgacgagc ttcttcaccc
     1861 ggtggtggac cccaacgccg agaagaaggc caagccgatc gcggccggtt tgcccgccgg
     1921 gccgggtggt gcttgtggcc aaatcgtctt caccagcgaa gacgccgtgg agtgggccaa
     1981 gaaagggaaa aaggtcatcc tcgtcaggga ggagaccaat ccggaggatg tggaaggtat
     2041 gcgggcggcg gaaggtatcc tgacggcccg tggtggtatg accagccacg cagcccttgt
     2101 ggcccgcggt tggggtaagt gctgtattgt cggtgccgga aagatcaaag tcgatgttga
     2161 aaagcggtgc atgacgattg acggcaaggt gctcaaggag ggtgattttg ttacgctcaa
     2221 cggaacgcgc ggccttgtct atgaaggtaa gctcggtctc attgacgcaa cagagaaccc
     2281 ccgcctcaag gagttcatgg cgcttgtgga gaaatatcga aagctcgggg ttcggacaaa
     2341 cgccgatacg ccagcagacg cgaaagtggc acgggacttc ggtgctgagg gtatcggtct
     2401 gttccggacc gaacacatgt tctatggaga aggttcggac gagccgctct tcatcttgcg
     2461 gaagatgatc ctatcgcgga cggccgaaga acaccgaaag gcgttggatg agttgttccc
     2521 cttcctgaag cgggacatca agagcaccct ggaggccatg gacgggttgc cggtgacaat
     2581 tcgcttgctt gacccgccac ttcac
//
LOCUS       DAOPTR010000563         2604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_31486_length_2604_cov_3.446842, whole genome shotgun sequence.
ACCESSION   DAOPTR010000563 DAOPTR010000000
VERSION     DAOPTR010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31486_length_2604_cov_3.446842"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2604
                     /locus_tag="PKI05_10685"
     CDS             <1..>2604
                     /locus_tag="PKI05_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52710.1"
                     /translation="GVAVYDEARHVWKSNQTNYGGTYRIGNVEVGDLVIAWTLIDANG
                     TQVGLGLGAWDSSVGGIVTKTIRAAESQVVDRVVAGNGVLAWVLRDRTDPNREQLNLA
                     IYDAGQRTWKQWATPSGMTQRIANIQIDGWVGWTVQKQDGQGNWVDKSACVAVYDPGR
                     GQWCLFQSPEATNRSVINFALAQGLAIWKIGYQQSSTSPYVARNVGAVSYDYETGTFQ
                     YNTTDYNPATQVELTAAGDGVAAWTAGSTVTVLAWDHLSGTWQTESVSYASDIRITGL
                     TTQGRLVAWKVESPTGYSNSYVARAVGYAAFDTLNQSWSVAEEDFGPSGLVTELFIEN
                     QFVHFTVDGVAQRRAYDAIGQMWSEDPATVISRFLLSPTAVPVNRGITTLDWSSGATN
                     GFIDYGDGWKSDSRYHIHAYRQAGIYTLTQTVWGPGGSDTSTAVIAALPARAAQDLGI
                     ISSLTLTDQVPTNSSLWYSFQVADDGAFTANVTGAGVGANTTMALCTLSGSLYSLVAT
                     GATSLAVSSVSAGTTYYLMIAGLEGNVDVALDNPPTLSLDDQVVTVRGTNSDDTAVLG
                     FDSFSGVLQAVVNDIPLCFEPGSTAVLFDGGAGNDQVRLQGTSGNETASLGTLVGNRP
                     GGIVTGTGFQLIAENCEDVTFLGGGGNDWAEIVDTPGRDFFQGGPGNSVLTGNGLRFA
                     AIDVATVHAYSRWGGDDVAEIRDSEGKDLLVAEPRWSRITSSDGSYFLRVKGFKTVRA
                     IAENGGVDTVQFRDSAGRDTFTVSPMNINDMASPAKAELVGPNYRISALGFENVEAQS
                     SGLGQDEAFLNGTRGNERVEATIGYAYLQAGGFAAKVNGFNQITVASMGGQDLAILRG
                     TPGDDTLVSQF"
BASE COUNT          540 a          727 c          778 g          559 t
ORIGIN      
        1 tcggcgttgc cgtttatgat gaagcccgac atgtctggaa atccaatcaa accaattacg
       61 gcggcaccta ccgtatcggc aatgtggaag tgggtgattt ggttatcgct tggaccttga
      121 tcgacgccaa tggcacacag gtcggcctgg ggctaggggc ttgggattcc tccgtgggcg
      181 gcattgttac caaaacgatt cgggcggccg agtcccaagt tgtcgatcgc gtggtagccg
      241 gtaacggcgt cctggcttgg gtgcttcgtg acagaaccga tcccaatcgt gaacaattga
      301 atctcgccat ttacgacgct gggcagcgaa cctggaaaca atgggcgact ccgtctggga
      361 tgacacagcg gattgccaat atccaaatcg acggctgggt cgggtggact gtccaaaagc
      421 aggacggtca aggcaattgg gtggataaat cggcctgcgt agccgtctat gatccggggc
      481 ggggacagtg gtgcctattc caatcgccgg aggccaccaa tcgctccgta atcaattttg
      541 cgcttgccca aggtctggcc atttggaaaa tcggttacca gcagtcgtca acaagcccgt
      601 atgtcgcccg caatgtggga gcggttagtt acgactacga gacgggcacg ttccagtaca
      661 acaccacgga ttacaatccg gccacacaag tggaactcac ggcggctggc gatggcgtgg
      721 ccgcctggac cgcgggcagc acggtcacgg tcttggcttg ggaccaccta agcggaacct
      781 ggcagacaga gtcggtgagt tacgccagcg atattcgtat caccggtttg accacccaag
      841 gccgactcgt ggcctggaag gtcgagtctc ccaccggtta cagcaattca tacgtggccc
      901 gagcggtggg ctatgccgcc ttcgacacgt taaaccaatc gtggtctgtt gcagaagaag
      961 attttggtcc ctcgggcctt gtgacggagc ttttcatcga gaaccagttt gtccatttca
     1021 cggtcgatgg agtggctcaa cgccgcgctt atgatgcaat cggtcagatg tggagtgagg
     1081 acccggccac ggttatttcc agatttctcc tatcgcccac cgcggtgcct gtcaatcgtg
     1141 ggatcacaac cctcgactgg tcgagtggtg ccaccaacgg attcatcgac tatggtgacg
     1201 gttggaagtc tgatagccgg tatcacatcc atgcctaccg gcaggccggt atttacacct
     1261 tgacacaaac cgtttgggga cccggcggca gtgacacctc cactgcggtc atcgcagcct
     1321 tgcctgctcg cgccgcccag gatttgggga ttatcagcag cctaacgctc acggaccagg
     1381 tacctacaaa ttcctcattg tggtacagct tccaggtcgc ggatgacggg gcctttacgg
     1441 ccaacgtcac cggtgccggt gtcggggcca ataccacgat ggccctttgc acgctaagcg
     1501 gcagcctcta ttccttggtg gccaccggcg ccacctccct agccgtgtct tcggtctcgg
     1561 ctggaacaac ttactatctc atgattgccg ggttggaagg caatgtcgat gtcgccctcg
     1621 acaatccgcc aactttgtcg ctggatgacc aggtcgtcac ggtgcggggg accaacagtg
     1681 acgacacggc ggttctgggc tttgacagct ttagcggcgt gctacaagcg gtcgtcaatg
     1741 acattccgct gtgtttcgag cctgggagta cggccgttct ctttgacggt ggagcaggaa
     1801 acgatcaggt gaggttgcaa ggaacgtcag gcaatgaaac ggccagcctg ggaacccttg
     1861 ttggcaatag acccggtgga attgtaacgg gcaccggttt ccagcttatc gcggagaact
     1921 gcgaagatgt taccttcctc ggcggtggtg gtaacgactg ggccgaaatc gtagataccc
     1981 ccggtcgaga cttcttccaa ggtggacctg gcaattcggt tctcacaggc aatggcctcc
     2041 gttttgcggc gatcgatgtc gctaccgtgc acgcctacag ccgttggggt ggcgatgacg
     2101 tggccgaaat cagggacagc gaggggaagg atctccttgt cgccgaaccc cgctggtcca
     2161 gaatcaccag ttccgatggt tcttacttcc tccgcgtgaa aggcttcaag acggtcaggg
     2221 ccatcgccga gaacggtggg gttgacacag tgcaattccg cgactccgcc ggccgcgata
     2281 cattcacggt gtcgcccatg aacattaacg acatggcatc gcccgcaaag gcagagcttg
     2341 ttggaccgaa ttaccgcatc tcggcactgg gctttgaaaa cgtggaggcc caaagttctg
     2401 gcctcggtca agacgaggcg ttcctcaatg gaacgcgagg caatgaacga gttgaggcca
     2461 ccattggtta cgcgtacctg caagccggtg gattcgcggc caaggtcaat ggtttcaacc
     2521 aaatcactgt cgcctcgatg ggcggtcagg atttggcaat ccttcggggt acccccgggg
     2581 acgatacgct tgtctctcag ttcc
//
LOCUS       DAOPTR010000564         2603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_31500_length_2603_cov_4.170330, whole genome shotgun sequence.
ACCESSION   DAOPTR010000564 DAOPTR010000000
VERSION     DAOPTR010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31500_length_2603_cov_4.170330"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PKI05_10690"
     CDS             <1..577
                     /locus_tag="PKI05_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52711.1"
                     /translation="EVAEIWWDKATQTTPPWQPRGMTLEGQAENATLQPGQELVDAFA
                     FQVPPEEFAQLYIRIPGRSVNLAEDFLFVIPKNEVQRPQDSDRAPSAGESDVESIGPM
                     SVTKQAEPPTAHADSPQAEVSPSDEDEEAIPIPGLSNAQGPASGEGGMDQEERTKLEE
                     LRKRGEALQEILDRQHENRRPGGSRPQSQRR"
     gene            658..1938
                     /locus_tag="PKI05_10695"
     CDS             658..1938
                     /locus_tag="PKI05_10695"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOA52712.1"
                     /translation="MNQIRKVVVFSVSFVFLFLWFSTRVMANENAVIWPRFRGPNGLG
                     VCDLYEFPAQIEPGNLAWQIELDGVGHASPVVAHGRVFIASANPETGEQILAAYATKD
                     GHLLWRHRLPGKTFPKHAFNSFASSTPTLDEKRLYYAWATPESLQLVAMQQENGEIVW
                     TKDLGPFVSQHGFGASPIVYKDTVILPNEQDGESFIVALECATGNIRWKTPRRSEKAA
                     YSTPTIFQKAGIKDQLILTSWAHGIASLDPETGTPLWELPIFHNRTVGSPLVVGDLIV
                     ASSGEGGIGREMFVVRAGIPETGRKPELHYEIRGSLPYVTTSVAYKGLLYLLFDKGIL
                     ICQRLNDGQSLWKERIAGEYFSSPLVLGGKLYCLSRSGMLVVVATGEKYRMLGQYDFG
                     EATHATPAVADGLLLVRTQSHLFAVKSIKSPPSK"
     gene            2127..>2603
                     /locus_tag="PKI05_10700"
     CDS             2127..>2603
                     /locus_tag="PKI05_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52713.1"
                     /translation="MMLVATAIFFFCLGWLANYRPVKQSGTPGKTVEGGTPVLLSGQL
                     RSGRPTGLPIPDAGGVVIVLPVGATPERPIPIEGLRPWDSNSAVGQINRQRLTELGGT
                     WTQTAEDGSYSVVLPRPGQYWVLAISKNLARPKEVLELTRRGIAELDYEQLSRYFER"
BASE COUNT          603 a          703 c          733 g          564 t
ORIGIN      
        1 tgaggtggca gaaatctggt gggacaaagc cactcagacg actccacctt ggcagcccag
       61 gggaatgact cttgaaggcc aagcggagaa cgcgacgctt cagcctggcc aggagttggt
      121 ggatgccttt gcttttcagg ttcctcccga ggaatttgca caactctaca tccgtatccc
      181 cggacgttcc gtaaatctgg cggaggactt tttgtttgtg ataccgaaaa acgaggtgca
      241 acggcctcaa gattcagatc gggccccctc ggcaggagag tccgatgtag agtcaatcgg
      301 gccgatgagt gtgacaaaac aagctgagcc tccaaccgct catgcggatt cgcctcaagc
      361 ggaggtgagc ccgtcagatg aggatgagga ggcaatcccc attccgggcc taagcaatgc
      421 ccagggaccg gcttccgggg agggaggaat ggaccaagaa gagcgaacca aactcgaaga
      481 actacgtaag cgcggcgaag cgctccagga gattttggat cgccaacacg aaaaccgtag
      541 gcctggtgga tcacgtcctc aatcgcaacg acgttgatcg aaaaagtagg aagagacatt
      601 tcatctcaag aggagataat cttcatagga cacgacgctc caaaggaacg gccgcccatg
      661 aatcagattc gcaaagttgt ggtcttctcc gtatcgtttg ttttcctatt cttgtggttt
      721 tccacgcggg taatggccaa cgaaaacgcg gtcatttggc cacggtttcg gggcccaaat
      781 gggttgggcg tatgcgatct gtatgagttc cccgctcaga tcgagccggg caacctcgcg
      841 tggcaaatcg agcttgacgg ggtagggcac gcctcgcctg ttgttgcgca tggtcgagtg
      901 tttatcgcct cggcaaatcc cgagacgggc gaacagattc ttgctgccta tgcgacaaag
      961 gacgggcact tactttggcg gcaccgccta cccggcaaga cattccccaa gcatgcgttc
     1021 aatagtttcg cttcatctac gcccactctg gatgaaaagc ggctttatta cgcctgggcc
     1081 actccggaaa gcctccagct cgttgcgatg caacaggaaa atggggaaat tgtttggaca
     1141 aaagacctcg gcccatttgt gtcgcaacat ggctttgggg catcacccat tgtttacaag
     1201 gatacggtca tcctgcctaa cgaacaggat ggcgagagtt ttatcgtggc tcttgaatgt
     1261 gccacaggta atatccgttg gaaaacgccg cggagaagcg aaaaggccgc atattcgacc
     1321 ccaacaatct tccagaaggc cggcatcaag gatcagctta tcctcaccag ttgggcgcac
     1381 ggcattgcga gtctggatcc cgaaacggga accccactgt gggaactgcc cattttccac
     1441 aatcgaactg ttggttcacc actcgtagtg ggtgacctca tcgtagcgag ttccggggag
     1501 ggaggaatcg gacgagaaat gttcgtcgtc cgtgccggga ttcccgaaac cggccgcaag
     1561 ccagagcttc actatgagat tcgtggttcg cttccgtacg taacaacatc tgttgcctat
     1621 aaagggttgt tgtacctttt gtttgacaaa ggcatcctca tttgccagcg tctcaacgac
     1681 ggccagagcc tttggaagga acgaatcgcg ggggagtact tcagttcgcc tctcgtcctc
     1741 ggaggaaagt tatattgtct ttcccgatcc ggcatgctcg tggtcgtggc aacgggggaa
     1801 aagtaccgaa tgctgggaca atacgatttt ggtgaagcca cgcatgcaac accggccgtt
     1861 gcggatggat tactgttggt gcgaacgcaa agccaccttt ttgccgttaa atcgatcaaa
     1921 tcaccaccgt caaagtgaac tcaggacaga acgaaaccaa acttgcggag ccatcgggga
     1981 tcagccccac gctaagggag agccctccgg ttgccccgcc cttgcgccag cctggccatg
     2041 gggcttcgtc ggcgccaaca gagaggcgcc aattatccgc tgccgatgtg atcgttgtgc
     2101 cgagaaaccg catacggtgg tactatttga tgttggtcgc gacggccatt ttcttttttt
     2161 gtcttggctg gctggccaac tatcggccgg ttaagcaatc gggtacaccg ggtaaaactg
     2221 tcgaaggagg tacgcctgtg cttctttccg gccaactccg ttctggccgc ccgaccggat
     2281 taccgatccc cgacgcggga ggcgttgtta tcgttcttcc tgtgggtgca actcccgaac
     2341 gtcccatccc tatcgaaggt cttcgcccct gggatagcaa ctccgcggtg ggccaaataa
     2401 accgtcaaag gcttaccgag ctcggcggta cgtggaccca aacggccgag gatggaagct
     2461 acagtgttgt gttaccccgg ccgggtcaat attgggtcct agcaatctcg aagaacctag
     2521 cgcgcccgaa ggaagtcctc gagctgacac ggcgtggcat tgctgaactc gattacgaac
     2581 aactttcccg gtactttgaa cga
//
LOCUS       DAOPTR010000565         2603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_31507_length_2603_cov_2.968210, whole genome shotgun sequence.
ACCESSION   DAOPTR010000565 DAOPTR010000000
VERSION     DAOPTR010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31507_length_2603_cov_2.968210"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="PKI05_10705"
     CDS             <1..799
                     /locus_tag="PKI05_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOA52714.1"
                     /translation="LVGALYPLTDQDLSECLNDGLPETLSEWIYANGLTHLKIKLNGD
                     DLRWDVDRVLAIHRVTSEAQRSRGCDTWHYSADFNERCESVDYVLEFMTRIRENSPEA
                     FDRLQYLEQPTHRDLKRFPENRMHKAAALKPVVIDESLVDFESLLLSRELGYSGVALK
                     TCKGHSEALLMAAAAQKFGLFLCVQDLTCPGYSFLHSASLAARIPGVAAIEGNARQYC
                     PSANRGWDEKYPTMFRITDGTVGTHVLDGIGLGFEPLFWSKEQIGSR"
     gene            complement(825..1343)
                     /locus_tag="PKI05_10710"
     CDS             complement(825..1343)
                     /locus_tag="PKI05_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOA52715.1"
                     /translation="MRAEFINPFIHSLYKTFSTMLNCSVQRGQLSLKTSSKTSYEISG
                     VIGLTGRAVGAVVLTLSKPVALKAASTMLLCDFSEINEDVIDAVGELANMVAGAAKAE
                     LEEYALVVSLPNVVTGKDHEIHFPSNVTPICIHFSSDWGPLALEVGLATIQNPSPEAA
                     TLTHHSEAVAAS"
     gene            1960..>2603
                     /locus_tag="PKI05_10715"
     CDS             1960..>2603
                     /locus_tag="PKI05_10715"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOA52716.1"
                     /translation="MNICIFLPNWLGDLVMAVPALRAIREATGSSAYVVGITRPNLAG
                     LLAGSQWLDEEWYFDPRGDDPRFETATLIEKLRSVHWDVGILLTNSFRSALLAVAGGI
                     RKRVGYSRHGRRLLLTHPVSVREIHGTRRYIPMVDYYLALAQAAGFPSQDRRLELPLS
                     PWGTVCADRIWRDLSLDSSDQVVAMHVAGAYGAAKSWPFSHFVELARMIATRTSA"
BASE COUNT          576 a          689 c          719 g          619 t
ORIGIN      
        1 cctggtgggg gcactttatc ctctgaccga tcaagacctg tcggaatgtc tcaacgatgg
       61 gttgccggaa acgttgtcgg agtggatcta tgcgaatggt ctcacacacc taaagatcaa
      121 gctcaatggc gacgatttgc ggtgggacgt tgatcgtgtt ttggcgattc accgagtgac
      181 gagcgaggcc caaagaagtc gcggctgtga cacttggcac tactccgccg atttcaatga
      241 gcgatgcgaa agcgtggact acgtcttgga gttcatgaca agaatccgcg agaattcgcc
      301 cgaggcgttc gaccgactcc aatacctaga acagcctacg caccgtgatc taaagagatt
      361 tccggaaaac cggatgcata aggcggcggc tttgaagccg gtggtcattg atgaatcttt
      421 ggtcgatttc gaaagcctgt tattaagtcg cgaacttgga tactcgggtg tggccctaaa
      481 aacgtgcaaa ggccacagcg aggccctcct catggcggca gcggcacaga agtttgggct
      541 gtttctgtgt gtgcaagatt tgacctgccc tggttattcc ttcttgcatt ccgcgtcgct
      601 ggctgcccgc attcccgggg ttgcggccat cgagggaaac gcccgtcagt attgtccctc
      661 cgccaatcga ggttgggacg agaaatatcc cactatgttt cggatcaccg atggaaccgt
      721 gggtacgcac gtcctcgatg gaattggctt gggctttgag ccacttttct ggagtaaaga
      781 acagatcggt tcgcgatgaa ggaattatct ttgtgaagtg ctcattaaga agcggcaact
      841 gcttcggaat gatgggtcaa agtcgcggct tctggcgagg ggttttggat cgttgccagt
      901 cctacctcta gtgcgagggg cccccaatcc gaagaaaagt ggatgcaaat cggcgtcaca
      961 ttcgaaggaa aatgaatctc atgatctttg cccgtaacaa cgttgggcaa acttacaacg
     1021 agggcatatt cttcaagctc tgcctttgcc gcaccggcaa ccatattggc cagttccccg
     1081 acggcgtcga tgacatcctc attaatttcg gaaaagtcgc agaggagcat cgttgacgcg
     1141 gcttttaagg cgaccggctt ggaaagagtt aataccacag cccccactgc gcgccccgta
     1201 aggccgataa ctccactgat ctcgtaagag gtttttgaac tggttttgag gcttaactgt
     1261 ccccgttgca ccgagcaatt gagcatcgtg gaaaatgttt tgtaaagcga atggataaaa
     1321 ggattgatga actcggcccg catagcacat cctttgttgc cactctaacg acccttcgcc
     1381 aaaaggttgg ctgttaacgc ctggctttcc ggcctcgtga taaattctcg atcgggttcc
     1441 ccaacatccc tcacatcact ccctcaagcc acgagaggga agttcggagc aatatgggtt
     1501 actcaattct agcccaaaaa gaaggagaca gcggactacc actagagcgg gggcgatttg
     1561 tgccaattac aaatgacgca aatgagcgtt tccgaagagg tctggaagcg acgttatccg
     1621 ttttgtatat tattttttga ggcctgttgc gtgtgtcgcc ggtttcgacc gtccgcctcc
     1681 ccgcggccga gacctgcagg accgcgcggc aggcttcttc attttgcaac gattcttttg
     1741 ccttttaagc agccaaaagt cttctcgcag ccgggaccga aaactaagag cactatagaa
     1801 ggacggcgta cctcgactcg ttttcctgga tggcgtagac cgctcttcca aattggtctt
     1861 ttccaaaatc ggaatcttgg ccaaaatgcc acctaaaggg cgattgatga cgagactgag
     1921 ggcagcagcc tgcccctaat tgaccgtgag gccgattcga tgaatatctg catcttcctc
     1981 cccaactggt tgggggatct ggtgatggcc gttccggcgc tgcgagccat tcgtgaggca
     2041 accggatcgt cggcatacgt tgtcggtatt acacgaccga acctggcagg tttactggcc
     2101 ggaagtcagt ggctcgacga agaatggtac ttcgatccgc gtggcgacga tccacggttt
     2161 gaaaccgcca ctctcatcga aaaactccgg tctgtccact gggatgtggg catcctttta
     2221 accaatagtt tccgctcggc gcttctggcc gtcgctgggg gaattcgcaa gcgggtcgga
     2281 tactcgcgtc atgggcgtcg cctcctgctg acccatccgg tgagtgtccg cgaaatccat
     2341 ggaaccaggc gctacatccc gatggtggac tactacctgg ccctggcaca agcggccggg
     2401 ttcccttctc aggacaggcg gcttgaactt ccattgtcgc cctggggaac ggtatgtgca
     2461 gaccggatat ggcgggatct ttcactcgat tcctctgacc aagttgtggc gatgcatgtc
     2521 gctggggcct acggtgcagc caaatcctgg ccattctcgc actttgtcga attggcccgg
     2581 atgattgcga cccgcacctc ggc
//
LOCUS       DAOPTR010000566         2600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_31546_length_2600_cov_3.596857, whole genome shotgun sequence.
ACCESSION   DAOPTR010000566 DAOPTR010000000
VERSION     DAOPTR010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31546_length_2600_cov_3.596857"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PKI05_10720"
     CDS             complement(<1..194)
                     /locus_tag="PKI05_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOA52717.1"
                     /translation="MSQGGQSNNEDAVLRGPDFSRNNGILPAIAQDAVTGEVLMLAYM
                     NEESFAETLASGRAVYFSRSR"
     gene            complement(494..>2600)
                     /locus_tag="PKI05_10725"
     CDS             complement(494..>2600)
                     /locus_tag="PKI05_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52718.1"
                     /translation="VWGQGRDKTILQAPSDPAIPFARFYPPAWDQGPTGVHTPGDTMV
                     YRVVFPRAGQWAVWMRYATDMSPWGQPGVSGRMTIALEDQPPVPMENLPNTGSFGTFR
                     WSRAAELVASAGEHRLTWKNVKGGGIHIDAFAFSDDPQWEPVEGQLPESGPHLVVVQG
                     EDVIAFQSKDGSLPGNEKACVWLAGDGGGLMHLTVLGSVQTNLGIVVRHPQYPQWIRN
                     CRIEDVLVKDCQGKQAENCGVRLFHAESAVVRGCELWGRSPLFLSGVRQCDFSGNRLV
                     SVTLWGGNAEGYINGRNETIEECIIEDNICAVPPGAQGGGPTGRRMIWFSTGRGSVSH
                     NWIAGNREDHAIFGGVAGTDQNVGEMILFEACQRIAYWGPIVTANSDNVTLPAKLAKT
                     PEEWLGNVKRESLAYDAEGNETPFYPPEEENEREPVLTQYYVTVLEGPALGQTRRIVA
                     RQGTTYHLDRPWQVVPPAGSRIVLSTAFYQNHIVKNRTVDGMTGIQLWISCIENIVSD
                     NEVARQRKPAVYLYGVCTTLASSMPMTWNRGIGPLYFNHIEGTRAEETSCGIFLTSGE
                     SAGLPLLFPRCLGNVLRHNGLVKNRTDGVLLIGNRKETKENGYTSVMGTIAEFNLVRD
                     ALCGFHVSNAVESTVLRRNHVYFWYPVSLGAKSPVAFQVDDADSTTVLELNSVEGISG
                     GLSQDIIPLLRGGERTDLP"
BASE COUNT          576 a          790 c          668 g          566 t
ORIGIN      
        1 cggctgcgac tgaaatatac agcccgaccg gatgccaatg tttcggcaaa gctctcctcg
       61 ttcatgtagg ccagcatgag gacttccccc gtaacagcgt cctgcgcgat cgctggcaag
      121 ataccgttat tacgcgaaaa atcggggccc cggagcaccg catcctcgtt attcgattga
      181 ccgccttggg acatcaccac aattcctttt ttctgacaac cacaattgtc atttcagtcg
      241 ttcagcatct ggcagaagtc ggcttcccgc ctgtcccgat attttgtccc cccgtggacg
      301 ttgaaaccat ccattttaac tgagtccggt caaaatccca ctgggaacgc gaaaagaggc
      361 aaaggcgtta ctcacccacc cggatggggc attcccgctc gaattatgca ttcggcttgg
      421 gaatggccaa tccgtttgac cgccgctttg ccagggcgaa tacgccaaat gccgaatagc
      481 atcttgaggg ctttcacggt aaatcggttc gctctccccc tcttagcagg gggataatgt
      541 cttggcttaa tcctcccgaa atcccttcca cggaattgag ctccaagacc gttgtagaat
      601 ctgcgtcatc cacctgaaat gccacgggag actttgcccc tagcgagacg ggataccaga
      661 aatagacgtg attgcgcctg aggaccgtac tttccaccgc gttgctgaca tggaaaccgc
      721 aaagggcatc gcggacaagg ttgaattctg caatggtgcc catgacggaa gtatatccat
      781 tttccttcgt ttcctttcgg ttacctatca acagaacgcc atccgttcgg tttttcacaa
      841 gtccgttgtg ccttaggacg ttacccaggc atcggggaaa gagtagcggc agtccggcgg
      901 actcgccgct ggtaagaaag atcccacagc tcgtttcctc ggcccgggtc ccctcgatgt
      961 ggttgaaata gagagggcca attccgcgat tccacgtcat gggcatggag gacgccaacg
     1021 ttgtacacac gccatacaaa taaacggctg gttttcgctg ccgcgccact tcgttatcgg
     1081 aaacgatatt ctcaatgcag gaaatccaaa gctgaatgcc cgtcataccg tccaccgtgc
     1141 gatttttaac aatgtgattt tgataaaagg ccgtggagag aacaatgcgg ctccctgccg
     1201 gtgggacaac ttgccaggga cggtccaggt gatacgtcgt gccctgccga gccacgatgc
     1261 ggcgcgtctg gcccaatgcc ggaccctcaa gcaccgtcac atagtattgg gtcagcacgg
     1321 gctcgcgttc gttctcttcc tccggaggat agaaaggcgt ttcattgcct tcggcatcat
     1381 aggccagact ctcgcgtttg acatttccca gccattcctc gggcgtcttg gcaagcttgg
     1441 cggggagcgt aacgttatcg ctgttcgctg tgacgatcgg tccccaatag gcaattcgtt
     1501 gacatgcctc gaagaggatc atttcgccga cgttctgatc ggttcccgcg acaccgccaa
     1561 agatggcgtg atcttcacga ttcccggcaa tccaattgtg cgagaccgat ccccgcccgg
     1621 tggaaaacca gatcatgcgc cgcccagtgg gaccaccacc ctgggcccca ggcgggacag
     1681 cacaaatatt gtcctcaatg atgcactcct cgatcgtctc gtttcggccg ttgatatagc
     1741 cctcggcgtt tccaccccaa agggtcacac taactaagcg attaccactg aaatcacatt
     1801 gccgaacgcc agaaagaaag agaggtgatc gtccccacag ctcgcatccc cgtaccacgg
     1861 ccgattccgc gtggaacaat cgcacaccgc agttctcggc ctgttttccc tggcaatcct
     1921 taaccaggac gtcttcgatc cggcaattac gaatccattg gggatactgc ggatgccgga
     1981 caacgattcc gagattcgtt tgcacgctcc ccaggacggt gagatgcatg agtcccccac
     2041 cgtcacctgc gagccagaca cacgcctttt cattccccgg gagacttccg tctttggact
     2101 gaaacgcgat cacgtcttca ccttgcacca cgaccagatg gggaccgctc tcgggaagct
     2161 gaccctcaac gggttcccac tggggatcgt cggaaaaggc aaacgcatcg atgtggatac
     2221 caccaccctt aacattcttc cacgtcaagc gatgttcgcc tgccgaggct accaactccg
     2281 ccgcccgact ccagcggaag gtcccgaaac tgcctgtgtt gggcaagttt tccatcggaa
     2341 caggcggttg gtcctcaagt gcgatggtca tcctgccgga aacaccgggt tgaccccatg
     2401 gcgacatgtc ggtggcgtac cgcatccaaa cggcccattg ccccgctcgt ggaaaaacca
     2461 cacgatagac catcgtgtca cccggcgtgt gaacacctgt gggcccttga tcccatgccg
     2521 gaggatagaa ccgtgcgaag gggatggccg gatccgatgg ggcctgaaga atggtcttgt
     2581 cgcgtccttg accccaaacc
//
LOCUS       DAOPTR010000567         2590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_31690_length_2590_cov_3.217357, whole genome shotgun sequence.
ACCESSION   DAOPTR010000567 DAOPTR010000000
VERSION     DAOPTR010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31690_length_2590_cov_3.217357"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1797)
                     /locus_tag="PKI05_10730"
     CDS             complement(<1..1797)
                     /locus_tag="PKI05_10730"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOA52719.1"
                     /translation="MGNLTTEYSHRGFFARYNLIILCAVTFAIPFMWIGAERALLSNK
                     NDVKSWLPDTYEETATFEWYRNHFESDMFILASWEGCTLDSPKLDLLARRLALDIQRS
                     SDDPYRFFKSVITGPQLINQLVEDQGLTREQAINRLRGFLVGQDGKQTCLVLVIDPEA
                     EARWEKHFGKNPRKKFLHAVLDSVYAAAKEICDIDPDELHLGGPPVDNVAIDVEGQRS
                     LVRLAIVCGAVGGLMAWWCLRSVVLTLLVFSTGVYSAGLSMILVWLTGTEMNAILLTM
                     PALVYVAAVSGAIHMANYYRDTLRTHEQWGLPDPAVGAPEKALKSAFLPIFLATFTTA
                     FGLASLSISELVPIQTFGIFSAAGIIVSFLFLCLYVPAMLQEWPVRNITKPRRTAFFD
                     PGRWSGWEHVGEIILRRGSWVVAGCILLMVLGAIGASQITTSVKLMRLFSPKARIIQD
                     YAWLEEKLGPLVPMEVVIKVDRDRAKLDLLDQIKLISEVQHAIQQLPDVGSVVAVPTF
                     ARPLPSRPGLVERRTWIVQLQRHREDLRDYWSEEESCELWRISARVKALTDLDYGVFV
                     NDIRQVVEPVLAKYRARSADGVTATYTGLVPLV"
     gene            complement(2257..>2590)
                     /locus_tag="PKI05_10735"
     CDS             complement(2257..>2590)
                     /locus_tag="PKI05_10735"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOA52720.1"
                     /translation="TVATFIGYVLAGHLGAIAATLGIITPCVILASLVHPWVVMARSS
                     EKIATFLNVINLVVIGLILSVCLNLGIWVFQDFKAIGFAVVAGVLLWFKVPPVLVVIA
                     AAVAGWLF"
BASE COUNT          695 a          719 c          630 g          546 t
ORIGIN      
        1 gaccagggga acgagacccg tgtaagttgc cgtgacaccg tccgcactgc gagcacggta
       61 cttggccagc accggttcga ccacctgtcg gatgtcgttt acaaagacac cgtagtcaag
      121 gtcggtgagg gccttcaccc tagcgctaat tcgccacaat tcacaacttt cttcttccga
      181 ccaatagtcg cgaaggtctt cccggtgccg ttgtaattgc acgatccacg tgcgcctttc
      241 taccaaacct ggccgagagg gcagtggtcg ggcgaacgta ggaactgcaa cgacgctacc
      301 cacatcggga agttgctgaa tcgcgtgttg aacctcactg atgagtttta tttggtcaag
      361 caaatcgagc ttcgcacggt cccgatcaac tttgatgacc acttccattg ggacaagggg
      421 tcctaacttt tcctctaacc aggcgtaatc ctggatgatc ctggcctttg gagagaaaag
      481 acgcattaat ttgaccgagg ttgtgatttg gctagcgcca atggcaccga ggaccataag
      541 caggatacac ccggcaacaa cccaggaacc ccgccgcaat atgatctcgc cgacatgttc
      601 ccatcccgac catcgtcccg gatcaaaaaa tgctgtccgc ctcggtttgg taatattgcg
      661 taccggccat tcttggagca tggccgggac gtagaggcac aggaacaaaa agctcacaat
      721 aatgccggcg gcagaaaaaa tcccgaatgt ctgaatcggg actagctcgc tgatagacaa
      781 tgaagccaat ccgaatgccg tggtgaaagt cgccaaaaat atcggcaaaa aagcactttt
      841 tagtgccttt tctggcgcgc ccacagccgg gtccggtaat ccccactgct cgtgtgttcg
      901 cagcgtgtct cgatagtagt tggccatgtg aatggcaccg gagaccgctg ccacatagac
      961 caaggctggc atggtcaaga gaatggcatt catctcggtc cccgtcagcc acacgaggat
     1021 catactaaga ccggcactgt agaccccggt ggaaaagaca aggagggtca aaaccacact
     1081 cctcaggcac caccaggcca taaggccgcc tacagcgccg cacacaatcg ctaggcggac
     1141 aagagacctt tggccctcga cgtcgatcgc aacgttatcc acagggggcc cgccgaggtg
     1201 aagttcatct ggatcaatat cacaaatttc tttggcggca gcatagacgc tatcaagaac
     1261 agcgtgaagg aactttttcc gtgggttttt accgaagtgt ttctcccagc gtgcctcggc
     1321 ctccggatca atcaccagga ccagacaggt ttgcttccca tcttgcccga caagaaaccc
     1381 tcgaagacga ttgatcgcct gctcccgagt gagaccctga tcctcgacta actgattgat
     1441 gagctggggc ccagtgatca cactcttgaa aaaacggtag ggatcatctg aggaccgctg
     1501 gatgtccaaa gctagtcgcc gggcaagcag gtctaacttg gggctatcca gcgtgcagcc
     1561 ctcccagctt gccaggataa acatatcact ctcgaagtga ttacgatacc actcaaaagt
     1621 ggcggtctcc tcataagtat cggggagcca tgacttgacg tcgttcttgt tgctaagtag
     1681 cgcccgctcc gccccgatcc acataaacgg gatcgcgaat gtaacggcac acaagatgat
     1741 aaggttatag cgggcaaaaa agccgcgatg agaatattcc gttgtcaaat ttcccatcaa
     1801 aagtaatcct tctcaatccc gggatgtatc ccccaatccg gtgtatctct tggtctctgg
     1861 aatgaagatc cggaataacg gctctcgctt tcgaccgtac ccgatgtcgt tccacaaaaa
     1921 tcagaaaact cctatgagtc ggtcaaggtt ttgagcgtct cccagatctt ggagacgagg
     1981 ccgcaggtca cagcgccttt ttcaataatt cggccgattc agcaggtaag acgggaagtg
     2041 aatgaattcg gtcggaatcg ttcctacaaa acaactacca aagcaaagcg acacgattgg
     2101 taaaaccgta atcgcttgac ggtgtccttt ttcttcgtat tcagtaaggc aaagccccac
     2161 aaaacggaag cagcacccta gcccacttca ttatgaccaa cctggtttaa ttctatgcat
     2221 caatggcgcc aactgcaacc gagtgaaatc ttcgcatcaa aacaaccatc ccgcgactgc
     2281 tgccgcgata acgaccagga ccggtgggac tttgaaccaa agcaacacgc cagcgacgac
     2341 cgcaaacccg attgccttga aatcttgaaa aacccaaatc cccaaattca ggcaaaccga
     2401 aagaatcaga ccaattacta cgagattgat gacatttaag aaagtggcaa ttttctctga
     2461 acttcgggcc ataaccaccc agggatgaac gaggcttgca agaatgacgc atggtgtaat
     2521 gatgccaagt gtagcggcaa tggcccctaa atggcctgcc aacacgtaac caatgaatgt
     2581 tgccacagtc
//
LOCUS       DAOPTR010000568         2588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_31710_length_2588_cov_3.506119, whole genome shotgun sequence.
ACCESSION   DAOPTR010000568 DAOPTR010000000
VERSION     DAOPTR010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31710_length_2588_cov_3.506119"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PKI05_10740"
     CDS             complement(<1..276)
                     /locus_tag="PKI05_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52721.1"
                     /translation="MVTQAKRLLLVLVAAATVLMATGCPKPATEQKPTRPSTTAGLEM
                     ETPSERPQAPVKEESAEPTAPSQTEPAAEPGQMAPTTEPAAEEKAAEK"
     gene            816..1778
                     /gene="fmt"
                     /locus_tag="PKI05_10745"
     CDS             816..1778
                     /gene="fmt"
                     /locus_tag="PKI05_10745"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOA52722.1"
                     /translation="MRLVMVGTGPFGVPTFEELYNTHHSVVCLVTAPLVRHGRPVPPS
                     PLRAIAAAHGTPIYDPEDINAPEARQYLEGLDADLLIVCDYGQILAPETLATARLGGI
                     NLHASLLPKYRGAAPINWAIYHGEKTTGVSVIHMTPQVDAGPIIAQGEVEIGPDETAP
                     EVEAKLAKIGAWFVRRALDNLEAGLLEALPQDPKLASKAPRLKKSDGLVDWTRPAEAI
                     RNQVRAFEPWPKTYTYWHRPGKPPLQLILGPVSVTHDVPPGVVPGTVVSAGKSGIVVA
                     AGEGGVVITQLQPAGRRMMSADEFIRGYHLQVGDRFGADASASK"
     gene            complement(2036..>2588)
                     /locus_tag="PKI05_10750"
     CDS             complement(2036..>2588)
                     /locus_tag="PKI05_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52723.1"
                     /translation="RFRSRVLACRENYRELYQAVHQYQNVNALAGAAGIAQANFAVAK
                     QFLTPPLSWSAIRRCPGMVVESSVNDGSRSVKVSESWVESETAFDREFGGVLLGYSPV
                     IHTASLGSAIPYEQVSLAAIPLWRDSPHPRDCQGWSWNHEGRGENWLFADGHVAFLPR
                     HRTNERPTVLQAVISSVSFTNSP"
BASE COUNT          528 a          765 c          727 g          568 t
ORIGIN      
        1 tttctcagcg gctttctctt cagcagcggg ttccgtcgtt ggcgccattt ggcccggctc
       61 tgcggcaggc tccgtttgcg agggcgccgt gggctccgcg gattcctctt ttaccggtgc
      121 ttgcggcctc tccgatggtg tttccatttc caagcccgcc gtcgtgctgg ggcgggttgg
      181 cttttgttct gtcgcaggct tgggacaacc ggtggccatt aggaccgtcg ctgccgccac
      241 caagaccaaa agcagccttt tcgcttgcgt taccataacg tgctcctcct ttccgaacac
      301 tcgatacgga ccagaagtca ttcccctccg cggaaccctc gtgtgaaatc aacgaactcc
      361 ccgaattctc aagctgctca aacgcgtcca cgccgttgaa gacattccgt gtcgtggcca
      421 gacgagtttg gcggagctaa aacattttcc ccgaaacgcc cccgcgatgc aactattatc
      481 tgtaaaaatt gaatcccggg gcacgacgtt tgaacccatc aggtcgcgag ccccacaagt
      541 atgagcgtca tcgtaagata ggggtgatat ttcatcatcg ggtatgtgtg ttccacttcg
      601 ggatggacgc ccccgtggct gccttttttt aatttggaag ccggatcgat caactcaggc
      661 gaaataggca aggcatcagc ccacaatggg gagtgccagt ggacaaatgg tggtcttccc
      721 cacgacaatg gtgttttttg tgtgatcctt aagtgctgac tcgtgttggt acgacgccta
      781 ttaagactcg ttgattcgtc gcgcggggcg ccggcatgcg gctggtgatg gtcggaacgg
      841 gtcccttcgg ggttcccaca ttcgaagagc tttacaatac ccaccattct gtggtgtgtt
      901 tggtcactgc gcccctcgta cgtcatggcc ggcccgtccc gccgagtccc ctccgggcaa
      961 ttgccgcggc ccatggtacg cccatttatg acccggagga cattaacgcc cccgaagccc
     1021 gccaatacct ggagggcctc gacgctgacc tccttatcgt ctgtgattat gggcaaatat
     1081 tggccccgga gacactagcg actgcccgac ttggcgggat caatttgcac gcttcgttgt
     1141 taccaaaata ccgcggcgct gctcccatca attgggcgat ttaccacgga gaaaagacca
     1201 ccggcgtcag tgtcattcac atgactccac aagtcgatgc cgggccgatc attgcccagg
     1261 gtgaggtcga aattggacca gacgaaaccg cacccgaagt cgaagccaag ctcgccaaga
     1321 ttggcgcctg gtttgtccgt cgcgccttgg acaacctcga agccggtctt ctcgaggccc
     1381 tgccccagga tccaaagttg gcttcgaagg ccccgcgtct gaaaaaaagc gatggcttgg
     1441 tggattggac ccgacctgcc gaggccatcc ggaatcaagt ccgggccttt gaaccctggc
     1501 cgaaaacgta cacatactgg catcggcccg gaaagcctcc cctacaactg atcttgggtc
     1561 ccgtgtcggt cacccatgat gtaccacccg gcgtggtgcc ggggactgtg gttagcgccg
     1621 gaaaatcggg catcgtcgtg gctgccggcg aaggcggtgt ggtcattacc cagcttcaac
     1681 ctgcgggccg tcgcatgatg tcggccgacg agttcattcg cggttatcac ttacaggtgg
     1741 gggatcgctt cggggcagat gccagtgctt caaaatgatt cagtcatcgt cgggacaaca
     1801 gcgcccgatt tctgtcggat atgacccagc taagagccta tcccataatc tcccctcgcc
     1861 cgtttggcgg gagaggggtc ggggttgagg gtggctggaa tcgctgccgg ttttctccgt
     1921 cgattccgac gtccgcttgc cctcacccca accctctccc ggagggagag ggggacttct
     1981 tgggaaaggc tttaagggga accctctcgc ggtgcagaat aatttcggca attggctagg
     2041 gagagttcgt aaagctgacc gaggagatga ccgcctgcaa taccgtgggc cgctcgttgg
     2101 ttcgatgtcg cggcaaaaac gccacgtggc catctgcaaa gagccagttt tctccacgac
     2161 cttcgtgatt ccaactccaa ccctggcagt cacggggatg aggactgtcc cgccataacg
     2221 gtatggccgc taaagacact tgctcataag ggatcgccga accaagagaa gccgtatgga
     2281 tcaccggcga gtagccgagc agtacgccgc caaactctct atcgaaagcg gtctccgatt
     2341 ccacccaaga ctcgctcact tttacggaac ggctgccatc gttaacgctg gattcgacca
     2401 ccattcccgg acagcgtcgg atagcggacc aagacaaggg cggtgtcagg aactgttttg
     2461 ccacagcgaa gtttgcctgg gcgataccag cagctcctgc caaggcatta acattttgat
     2521 actgatgaac ggcttgatac agttcccgat agttctcacg acatgcgagg accctcgagc
     2581 ggaaacga
//
LOCUS       DAOPTR010000569         2587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_31719_length_2587_cov_6.111374, whole genome shotgun sequence.
ACCESSION   DAOPTR010000569 DAOPTR010000000
VERSION     DAOPTR010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31719_length_2587_cov_6.111374"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..395
                     /locus_tag="PKI05_10755"
     CDS             213..395
                     /locus_tag="PKI05_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52724.1"
                     /translation="MTGNIDVLLEKLSPGSLAHELVSKLKDSMRDEWPEVLKASLNAR
                     LTQKVGELMHGQDQAS"
     gene            373..>1726
                     /locus_tag="PKI05_10760"
     CDS             373..>1726
                     /locus_tag="PKI05_10760"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOA52725.1"
                     /translation="MVRIKRLEIKGFRAFGAQPQMLDFSGPMAVVWGPNSQGKTSLAE
                     AIEFLLTGKTVRRELVASAKREFAEALRNAHLPLSEDVVVAAEIEDAQGQVHRIERRL
                     IRDYTGRDPCQSELTIDGNLADDLTCIGIRLSQPPLEASVLMPHTLRYVVSADPQQRT
                     EYFKAILEVSDLEEIRGAIVDAKNQLVEPISDVSPVYERCRSNPQFGHKLALLETESP
                     SRKLVENLLSEALEQILSGHSQVPPGLDARVSLAKELLKRQREQTFPLDDLALRSEPS
                     WEARPDVRRPLEAFLRAKTAVDKEVTRLLSLFEEVLKIPDITSATKPIDCPVCGTPQA
                     LTPERINAIRQKVESNKQFTKHRQCAENVLHDLTVAAQRLLTEAKDACPSAMNWQTQD
                     WSRHEPAIAELLGDRAAGIMTPWREALSDLATALGSLQDKAIALQNTVRSPRLDDLDE
                     A"
     assembly_gap    1727..1736
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1745..>2587
                     /locus_tag="PKI05_10765"
     CDS             1745..>2587
                     /locus_tag="PKI05_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52726.1"
                     /translation="MNDLFQSAEHFRNALGRYQKAVEPLLAALKQEIDRRAGTEGWQD
                     LIDLIVQREKFLQWLIERAAYREILKEVDQAIGEIDRAKAEILDDKFAELSQEIARWW
                     NLLRPDEPTSFHGLVRAGTGRRFIDLKARLATADPSASQSVLRDAVAVFSDSQLNCLG
                     LAAFLARTVREGTGFVILDDPVSASDEEHRAFFIDRVLDALIQSGIQVILLTHDERMW
                     KEVLERYSHLDLNTFIVKLEDPAKGTAVEERSDTLDAMLARATLYVGNPNPESRKIAA
                     RILRD"
BASE COUNT          590 a          723 c          751 g          513 t
ORIGIN      
        1 agtacagcct ttagaggagg cccttgtgca gttgcaggaa gcggaagagg aacggagaga
       61 ggccgaccgc gaactggcgg gggttctgaa aacacttggg tttggggggt tcgatgtcta
      121 ataagatgtc taataataag agaccacaaa taggacaaac ggacgacggg gattgcccgc
      181 aaccgagctc agacctgagc aaagcacgcg tcatgaccgg gaacatcgac gtgctgcttg
      241 aaaagctctc tccggggagc ctcgcccatg agctcgtctc caagctgaaa gactccatgc
      301 gagatgagtg gcctgaggtc ctgaaggcgt ctctgaacgc ccgtttgact cagaaggtag
      361 gggagctaat gcatggtcag gatcaagcgt cttgagatta aaggcttccg cgccttcgga
      421 gcacagcccc agatgctgga ctttagtggt ccgatggctg tcgtctgggg tccgaacagc
      481 caagggaaga cgagtctcgc cgaggctatt gagtttctac tgacaggcaa gactgtccgc
      541 cgcgaattgg tcgcaagcgc gaaacgcgag tttgccgaag ccttgcggaa tgcacatctt
      601 ccactgtctg aagacgtcgt tgtggccgcc gagattgagg atgcccaggg gcaggtgcat
      661 cgtatcgagc gtcgcctgat ccgcgactac acaggtcggg atccctgcca gagcgagttg
      721 accatcgacg gtaacctcgc tgatgacttg acatgcatcg ggattcgtct ttcgcagccg
      781 ccgctagaag cctcggtgct gatgccccac acgctgcgtt acgtcgtctc tgctgacccc
      841 cagcagcgaa cggagtactt caaggccatt ttggaggtct cagatctcga agaaatacgg
      901 ggcgcaattg ttgacgccaa gaatcagctg gtggaaccaa tcagcgacgt ttcgccagtc
      961 tatgaacgtt gccgttcaaa cccgcaattc ggccacaaac tggcattgct cgaaacagag
     1021 tcaccctcgc gcaaattagt tgagaattta ctgagtgaag cactggaaca gattctttct
     1081 ggccacagtc aggtgccccc cggactcgac gcccgagtgt cactggctaa ggaactcttg
     1141 aagcgccagc gcgaacaaac ttttcccctc gatgatcttg ccctccgttc ggagccgtcg
     1201 tgggaggcaa ggcccgatgt ccggcgaccc ctggaagcat tccttcgggc aaagaccgca
     1261 gtcgataaag aggttactcg gctcctatcg cttttcgagg aagtgctcaa gatcccggat
     1321 atcacctcgg cgacaaagcc catcgattgc ccggtctgcg gaactcccca ggcccttacc
     1381 cccgagcgga tcaacgcaat ccgccagaag gtcgaatcga acaagcagtt tacaaaacac
     1441 cggcagtgtg ccgaaaacgt cctgcacgac ctaaccgtgg cagcgcagag gttgctcacc
     1501 gaggcaaagg atgcctgccc atcggctatg aactggcaga cgcaagattg gtctcgccat
     1561 gaaccagcaa tagcagagct tctcggcgac cgagcggcgg gtattatgac tccgtggcgg
     1621 gaagccttat ccgaccttgc aacagccctc ggcagcttgc aggacaaggc tattgccctc
     1681 cagaacacgg tgcgctcccc tcgacttgac gacctcgacg aggcaannnn nnnnnngtca
     1741 ggaggtgaac gacctattcc aatctgccga gcattttcgc aacgccttgg gccgttatca
     1801 aaaggccgtc gaacccctcc ttgctgcctt gaagcaagaa atcgatcgac gggcaggaac
     1861 ggaaggatgg caggatttga ttgacttaat tgtgcagcgt gagaagtttc ttcagtggct
     1921 tattgagcgt gcggcctacc gcgagatcct gaaggaagtg gaccaggcca ttggggagat
     1981 cgatagggcc aaggccgaaa tcttggacga caagttcgcc gagctgagcc aggagattgc
     2041 ccgctggtgg aaccttctca ggcctgacga accgacatcc ttccacggcc tggtacgcgc
     2101 tggcaccggc aggcgcttta ttgatcttaa agcacgcctc gccactgcag acccttcggc
     2161 ctcgcagagc gtgctccggg atgccgtcgc ggtgttcagc gactcgcagc tcaattgctt
     2221 gggtctcgcg gccttcttgg cgaggacggt tcgagaaggg acgggcttcg ttatcctgga
     2281 cgatcccgtt tccgccagcg acgaggaaca ccgggccttc ttcatcgacc gggttctcga
     2341 cgcgctgatc caatccggga tccaggtcat cctgctcaca catgacgaac gtatgtggaa
     2401 ggaagtgctg gagcgctaca gtcatttgga tttgaacaca ttcatcgtga agctggagga
     2461 tcctgccaaa ggcacggcag ttgaggagcg gagtgatacg ctcgatgcca tgctggcacg
     2521 cgctactttg tacgtcggta atccaaaccc tgagagtcga aaaatcgcag ctcggattct
     2581 acgggac
//
LOCUS       DAOPTR010000570         2580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_31814_length_2580_cov_3.760000, whole genome shotgun sequence.
ACCESSION   DAOPTR010000570 DAOPTR010000000
VERSION     DAOPTR010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31814_length_2580_cov_3.760000"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /locus_tag="PKI05_10770"
     CDS             <1..1234
                     /locus_tag="PKI05_10770"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOA52727.1"
                     /translation="SGPALASLIDESRSWLRVARCAVLVNSLLVLTIFYLTYRCYDGS
                     LYHAFVKFGYIPLRDGSFGANPNQLGGQLAFASVLGLAIFFRSGRAPEKEGRVTPLFI
                     QGRLPCGAFSAHRESVSRILPPAAGGVVPAKSARPVRRFTFVKADWLVILASLVTAGG
                     CLMTGSRGAVLSLIIGAFVVIGGSAGLQPLHRLRDFAVIVGMSLLMGTLATTVLPVNP
                     VIRLIARFSEGDATNIATAGSRLSIWENALRAWTSNPGRILVGTGTGGADVALGELDD
                     GATWDDSGVLRRNCHNAFLEWLLSFGIVGLIPGVSFVLFLWLRASRLDHAEGATIRTG
                     LLATIFAFGMTSVVYRHLCWPLEAGLMLALLCQPENACQSVPSPNKRLTQRIDSHHPI
                     PTPHVGKTFHAGYQPSLR"
     gene            1294..2427
                     /locus_tag="PKI05_10775"
     CDS             1294..2427
                     /locus_tag="PKI05_10775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA52728.1"
                     /translation="MNILHVAPIDSNKISGIRQNAVSLAKAQNLLEGTRAGLLISSDD
                     PHPWQLPGIPTITKHTLTKGCPRSQWIERIAQAVCYPDLVVFHSTFIPFHAWLGEQLT
                     LRGIPYIICPRGGMTREALGQRAWKKWLGRRLFFDQFVAGAAAIQFLRQRELERSGDW
                     GRPMIIAGNGVWLPDENLCARPGTHQQRIFLFLGRLDIHIKGLDVLVEACQRCQSVLR
                     HHGTVIRLVGPSFRGSGEWLEKEIRARGIGDIVQLFGAVGAEERSRVLAEADLLLQPS
                     RTEGHPASVIEALAHGLPVLISPETNMLNEVLAARAGWGVSVHPLELARELVRLSLVR
                     GEVLAEAGRAARRYAVRELSWQRVAAKTAEKYRQILRVVRRAA"
BASE COUNT          487 a          697 c          775 g          621 t
ORIGIN      
        1 aagtggccct gccctcgcct ctttgattga cgagtctcga agctggttac gggtggcgcg
       61 gtgtgcagtt cttgtcaata gtcttcttgt tttgacaatc ttttacctaa cttaccgctg
      121 ttacgacggc tcgttgtatc atgccttcgt caaatttggt tatataccgc ttagggatgg
      181 aagcttcggg gccaatccga atcaacttgg gggacaactg gcctttgcca gtgtacttgg
      241 gctggcaata ttctttcggt ccgggcgtgc cccagaaaag gaggggcgag tcactcctct
      301 tttcatccaa gggcgtttgc cttgcggggc attttcagcc catcgagagt cagtgtcccg
      361 gatcttacca cctgcggcag gcggggtggt tccggctaag agtgcgcgac cggtacgccg
      421 gtttacattc gtgaaggccg actggcttgt gatcttggcc agcctggtca cggcgggcgg
      481 atgtcttatg acgggttccc ggggtgctgt cctgagcttg attatcgggg cgtttgtcgt
      541 catcggtgga agtgctggcc ttcagcccct tcacaggttg cgggattttg ccgtcattgt
      601 tgggatgagc cttctcatgg gcactttggc cacgaccgtc ctgcctgtaa accccgtcat
      661 aaggctgatc gcccgttttt cggaaggtga tgctaccaac attgccacag ccgggagtcg
      721 cttgtccatt tgggaaaacg ctttgcgggc gtggacgagc aacccaggac ggattcttgt
      781 gggcaccgga acaggtggtg ccgatgtggc cctcggcgaa ctcgatgacg gggccacttg
      841 ggacgattcc ggcgtgctgc gccgaaactg tcacaatgcg tttttggaat ggctgctgag
      901 tttcggcatt gttgggctga ttccgggcgt gtcttttgtg ttgttcttgt ggttgcgtgc
      961 gtctcggttg gaccatgcgg aaggggcaac gatacgaaca ggccttttgg caacaatctt
     1021 tgccttcgga atgacaagtg tcgtgtaccg tcacctatgt tggccgctgg aggccggttt
     1081 aatgctcgcg cttttgtgtc agccggaaaa cgcttgccaa tccgtcccca gtcccaacaa
     1141 gaggcttaca cagcggatcg attcccatca tcctattcct acaccgcatg tgggaaaaac
     1201 atttcatgcc ggttatcaac cgtcattgcg ttagccgata tacctttagc cacaccgtaa
     1261 ccatctttaa agctgcttgc tgacagccct gccatgaata tccttcacgt cgctcccatt
     1321 gattcaaaca agatcagcgg cattcgtcag aatgcagtca gtctggcgaa ggcgcaaaac
     1381 ctccttgagg gtactcgcgc cggtttgctg atcagttcgg acgacccgca tccatggcag
     1441 ctgccgggga ttccaacgat aacgaaacac accctgacca agggttgccc gcggagtcag
     1501 tggatcgaac ggatcgccca ggcagtctgt tatcccgacc ttgtagtttt ccacagtacg
     1561 ttcattccct ttcatgcgtg gcttggtgag cagttaacgt tgcgtggaat tccttacatc
     1621 atttgtcctc gcgggggaat gacccgggag gccttgggac aaagggcgtg gaagaaatgg
     1681 cttggtcgtc gtctgttttt tgatcagttt gtggccggag ctgctgccat tcagtttctt
     1741 cgacagcggg aactcgaacg atcgggagat tggggacgcc ccatgatcat cgccggaaat
     1801 ggtgtttggc ttccggatga aaatctgtgt gctcggccag ggacccatca acagcgaatc
     1861 tttcttttcc tcggccggtt ggatatccac atcaaggggt tagacgtgct ggttgaggcc
     1921 tgccagcgct gccagagcgt gttacgccat catgggacgg tgatccgtct ggtggggcca
     1981 agctttcgcg gctctggtga gtggctggaa aaggaaatcc gtgcccgggg tatcggcgac
     2041 atcgtccagt tgtttggtgc ggtgggcgca gaggaacgat ctcgggtttt ggccgaggcc
     2101 gacctgctcc tgcaaccatc ccgaacggag ggccacccgg cgtccgtgat cgaagcccta
     2161 gcccacggtt tgccggtgct gattagtccc gaaaccaaca tgctcaacga ggtccttgcc
     2221 gcgcgggcgg gatggggtgt gtccgtccat cccctagagc ttgcccgcga acttgtccgc
     2281 ctgagcctcg tccgcggaga ggtccttgcc gaggcgggtc gggcagctcg tcgctacgcc
     2341 gtgagggaac tgagttggca gcgcgtggcg gcgaaaacgg cggagaagta tcggcaaatc
     2401 ctgcgggtcg tccgtcgcgc ggcataatca cggcaggtta ccccgcgctc acttcaaaac
     2461 actcaacgac ggtcttcgcc aggattaagt gtgcaaaaag cataaaaaaa gcggccaaga
     2521 cgcaacgtct cagccgccaa tgcccactag cgacagggct gcgtgagggg aaatccccgt
//
LOCUS       DAOPTR010000571         2579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_31832_length_2579_cov_3.024960, whole genome shotgun sequence.
ACCESSION   DAOPTR010000571 DAOPTR010000000
VERSION     DAOPTR010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31832_length_2579_cov_3.024960"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..432)
                     /locus_tag="PKI05_10780"
     CDS             complement(172..432)
                     /locus_tag="PKI05_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52729.1"
                     /translation="MIHLVAAILLVSVQFLGCAMAIATRFPGTRDWASTGFHGILALL
                     AGIALAACGQSHHVWGSCLVTVSVMILLSMADFTQPTDRKSV"
     gene            770..2257
                     /gene="pap"
                     /locus_tag="PKI05_10785"
     CDS             770..2257
                     /gene="pap"
                     /locus_tag="PKI05_10785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HOA52730.1"
                     /translation="MLELVDLNQRISGEEYEKIFPDLEAQLAACQRMARQSGIPIAIV
                     FEGWDAAGKGMMINRLAQVLDPRGFRVYLTGDPSAEEQRRPWMWRFWKELPGAGEIVM
                     FDHSWYRRILDDVAQGRLSRKEYQQAVHDILEFEKQLADSGLLLIKFWLHISAKEQRR
                     RLKRLEQSPATRWKVTKSQWEQNRKYKKWQKLVETALQRTSSSQNPWTVVEANQGKFA
                     RVKVFRTIISAIKEACARREREPAPSLTPMPEPDESQFYRPTILDRVDLTKSIGREEY
                     ERELEVLHKKLYELEHRCYLAKIPVVIVYEGWDAAGKGGTIKRLTRALDPRGYEVVPV
                     GPPTTEEKAHHYLWRFWRRIPKTGHITIFDRSWYGRVLVERVEGYCSQEEWERAYREI
                     NEFERQLADFGTVIIKFWLHIDSDEQLRRFERRQSDPQKQWKITEGDWRNRAKRKHCE
                     LAVIDMLQRTSTLYAPWTILEANCKLFARIKSLRVVVERLEAATR"
BASE COUNT          641 a          638 c          755 g          545 t
ORIGIN      
        1 accgtacttc ccgcctggca gttttaacag cgcatcattt gggcgacgcg aggggggatt
       61 aaagccacaa gaggacactt catcgcacaa gaggacactt tagcccgatg aattcgcctt
      121 ttacggaagg gaaggcccaa gaggaccggg caactgggtt aagaaacttg gtcagacgga
      181 cttcctatcg gtaggctggg taaagtcggc catactgaga aggatcatca cggagacggt
      241 gacgaggcag gaaccccaca catgatggga ttggccacac gctgccagag ctattccagc
      301 caataacgct aggatgccat ggaaaccggt actcgcccag tcccgcgtac cgggaaagcg
      361 agttgcgatg gccatcgcgc agcccaaaaa ctggacactt acaagtaata tagcggccac
      421 aaggtggatc acaccgtccc tcatcgcttt aagctgccga acacccttga cttgccacgg
      481 cttggacgaa cgagagaagg atagcttaaa ccttttcgcc gataaagcct gattttcgcg
      541 atttgctggc aaaggcttat tcactttgaa tctgaacttt cccaaattgc ccaggctgac
      601 ttttcgcctc acgtgatgtg ttaggtactt catgctatgg ttacggggga ccatgaggcc
      661 gctttcccat tccgcgggtg cgcgctattt ttgatccgtt agcggaccac atctaatact
      721 cgaaagaacc gcgaggaaag aggtgtgggg aaaataggcg aggtgcgcca tgttggaact
      781 ggttgatctc aaccaacgga tttctggcga ggagtacgaa aagatattcc cagacctgga
      841 agcgcagctc gcggcgtgtc agcggatggc ccggcagtcg gggataccca tcgcgattgt
      901 ctttgagggc tgggacgcgg ctggaaaggg aatgatgatt aaccggctcg cccaggtctt
      961 ggacccccgc ggatttcgcg tttatttgac gggggatccg agcgccgagg aacaacgccg
     1021 tccgtggatg tggcggttct ggaaggagct gccaggggcg ggggaaatcg tcatgttcga
     1081 ccactcttgg taccgccgta tcctcgacga cgtggcccag gggcgacttt cgaggaagga
     1141 ataccagcag gcggttcatg atattctcga atttgaaaaa caactggccg acagtgggtt
     1201 gctcttgata aagttttggc ttcacatcag cgccaaggaa caacggcggc gtctaaagcg
     1261 gctggagcag tcgcctgcta cacggtggaa agtgaccaaa tcacagtggg agcagaaccg
     1321 caaatacaag aagtggcaaa aattggtcga gacggctctc cagcggacga gctcctctca
     1381 aaacccatgg acggtggtcg aggccaatca ggggaagttt gccagagtca aagtctttcg
     1441 cactatcata tctgcaatca aagaggcgtg tgcccggcga gagcgagagc cggcgccttc
     1501 gttgacacct atgccggaac cagacgaaag ccagttttac cggcccacga ttttggatcg
     1561 tgtcgatttg actaagtcga ttggccgaga ggagtacgaa cgggaactcg aggttcttca
     1621 taaaaaactc tatgagctgg aacaccgttg ctacttggcg aagataccgg ttgtgatcgt
     1681 ttatgagggt tgggacgctg cgggaaaggg tggcacaatc aagcgactga cccgggcgct
     1741 ggacccgagg gggtatgaag ttgtccctgt tggacctccc acgaccgaag aaaaagccca
     1801 tcattatcta tggcggtttt ggcgacgcat tcccaagacg ggacatatca cgatctttga
     1861 ccgctcctgg tacggacggg tgttggtaga acgcgttgag ggctactgct cgcaggagga
     1921 atgggaacga gcttaccggg aaattaatga atttgaacgc cagttggccg actttggcac
     1981 ggtaatcatc aagttctggc ttcatatcga ttctgatgaa caacttcggc ggtttgaaag
     2041 gcgacaatcc gatccccaga aacagtggaa gattaccgaa ggggattggc gcaatcgggc
     2101 taaacggaaa cattgtgagc tggccgtgat cgacatgctt caacgaacga gcaccctgta
     2161 cgcaccgtgg acgattctcg aagcgaactg caaactcttc gccaggatca aatcgcttcg
     2221 tgtcgtggtt gaacgtctgg aagctgccac acgttgattc aagggaggca atcattgggg
     2281 cggggcccgc ggcgccacgg ccttcgccgg tggttttttg gggagcaggg gatattcacg
     2341 gttcaaggca aatagacgat actgttgtgt cggtcgctta atggggcaac ccaccgttta
     2401 cccgcatgcg tctgaagatc gcacagaaca aaattagtca gaatgcatta gataatctcc
     2461 ctgcagttta ccccccaaca ctgaagaaag gcgttagcac caagaaaggc tgttaccgtg
     2521 ctcgaaattg tcgctagtct gttgcttgtc cttggagtgg ccgaatcaaa ggtcaacga
//
LOCUS       DAOPTR010000572         2576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_31877_length_2576_cov_2.549782, whole genome shotgun sequence.
ACCESSION   DAOPTR010000572 DAOPTR010000000
VERSION     DAOPTR010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31877_length_2576_cov_2.549782"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PKI05_10790"
     CDS             complement(<1..546)
                     /locus_tag="PKI05_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOA52731.1"
                     /translation="MSRGGSSGAAGSKVLLLCRCDVEFGDEIYVARGLSIGRTPDNTF
                     VIDDPAVSRHHCQVEWDGAGGLILRCVDPGAVLEYKGGRVRELRLEAGLRFRVGPAEF
                     ECRLGGLGAKAVPRWVGQCPYCQSTDLPAPAPNVQACPACRRQVLVFRPRFSSGVACV
                     AGEFGGVRLVRLVGEGGMSVVF"
     gene            892..2385
                     /locus_tag="PKI05_10795"
     CDS             892..2385
                     /locus_tag="PKI05_10795"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009094781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOA52732.1"
                     /translation="MALEKFQLLPEVRDFLAKKRFGGVIGGQEVFASNGETFHTRDPG
                     TGDVLAEVANMQPDDVDQAVQAAAKAFRQTNWPNLTAHERGAILHRWVDLVESKKRII
                     AQIESLDCGKIYRQAEADVQNFIDTMRYFIEMSLHIERRAVLAVPHYEAYSYRAPWGP
                     CGFIFPWNFPILLVGWGIAPALAAGNTVVIKPAEDTPLSTLFVAHLAKEAGIPDGVVN
                     VVPGLGEVTGAALARHPGLRRMSFTGSPEVGRLVGEACARNLVPVKLELGGKGAAVVF
                     DDVDVSATVEKLVQAVTFHCGQVCCTATRWIIQKTIYDRFVSEAVKRLQEIRIGHELE
                     PRVQMGPVVNEKQKKRILSYIERGRAEGAEVILEGGELELPELGGFFVKPALLAGSLK
                     NVAAQEEIFGPVAYLTSFSDEADAIEKANDTEYGLANSVWTADLQRARRVAEAMEAAN
                     SWINAHNLFPHGVPYAGIKKSGLGGGVLSEQTYFDYLRSTSVVRPVS"
BASE COUNT          548 a          723 c          766 g          539 t
ORIGIN      
        1 gaacaccacc gacatcccgc cttctcccac cagtcgcacc aagcggactc cgccaaattc
       61 cccggccacg caggccactc ccgaggaaaa ccgcgggcga aagacaagca cctggcgacg
      121 acacgccgga caggcctgga catttggcgc tggtgcgggc agatcggtgc tttggcagta
      181 gggacactgg ccaacccacc gaggcaccgc ttttgccccc agacctccaa ggcgacactc
      241 gaactcggct ggtccaaccc gaaaccgcag ccccgcttcg agccgcaact ctctcactcg
      301 gccacccttg tactctagca ctgccccggg atcaacacaa cgcaagatca ggccgcccgc
      361 cccatcccat tccacctggc agtggtggcg ggacaccgct gggtcatcga tgacaaaggt
      421 gttgtctggc gttcgaccga tcgacagccc ccgggccaca tagatctcgt ccccaaattc
      481 cacgtcgcag cgacaaagta atagcacctt gctcccggca gcacctgatg accctccgcg
      541 actcatttct gcctcctcgt ggtggcccgc ccgcgactct gaatagattg actccggcca
      601 caaggtcaaa cctgccaact tgccctaatt ctaattggct gagcttttgc atgtcaacaa
      661 actgcctgga ggagctcctt agatgccgca ttgcgtggcg acatttgcat gatggctttg
      721 caaggccttc aatgatccca ggcgggacat tgcaggcggg ccggggtagc cggttgactg
      781 actccccgtt cttctgggcg tttgatatat tagagaccga aacctaaccg gcgaatcggc
      841 gtgtgattcg ggggactcct gggactcgct tagccacgaa aggaaaagac gatggctctg
      901 gaaaaatttc aactgctccc agaggtgcgc gatttcttgg ccaagaagcg ttttggaggt
      961 gtgatcggag gccaagaggt ttttgcctcc aacggcgaga cgttccacac gcgggaccct
     1021 gggacgggag acgtcttggc cgaggtggcc aatatgcaac ctgatgacgt tgaccaagcc
     1081 gttcaggctg cggccaaggc ctttcgccaa accaattggc caaacctaac ggctcacgag
     1141 cgaggggcta ttctccatcg gtgggtggat ctggtggaaa gcaagaaacg gatcattgcc
     1201 caaatcgaat cactggattg tggtaagatc tatcggcagg ctgaggccga cgtccagaac
     1261 tttatcgaca cgatgcgata tttcattgaa atgtcgcttc atatcgagcg ccgggctgtt
     1321 ctggccgtgc cgcattacga ggcttattcc taccgtgcgc cgtggggacc gtgtgggttc
     1381 atctttccct ggaactttcc aatcctcctg gtcggttggg ggattgcccc ggcgctggcg
     1441 gctggaaata ctgtggtcat caaacccgcc gaggataccc ctctttccac attgtttgtt
     1501 gcgcacttag ccaaggaggc cggtattccc gatggcgtcg tcaacgtggt gccgggttta
     1561 ggagaagtaa cgggcgcagc gttggcgcgg catcccgggc taaggcgaat gtcattcaca
     1621 ggctcgccgg aggtcggacg gctcgtgggt gaagcctgtg cccgaaattt ggttcccgtt
     1681 aaactcgagc tgggaggtaa aggggctgcg gtggtctttg atgacgtcga tgtgtcggcc
     1741 accgttgaaa agctggtcca ggccgtgacg ttccattgcg gacaggtgtg ctgcacagcg
     1801 acccgctgga tcattcagaa aaccatctac gatcggtttg tgtccgaggc cgtcaaacga
     1861 ctacaagaaa tccgcatcgg ccacgagcta gaacctcgag ttcagatggg accggtggtt
     1921 aacgaaaagc agaaaaaacg gattttgtcg tacattgaac ggggaagagc ggagggggct
     1981 gaagtcattt tggaaggcgg ggaattggag ttgccggagt tgggtggctt cttcgtcaag
     2041 ccggcactct tggcaggttc gctgaagaac gtagcggctc aagaggagat ttttgggccc
     2101 gtcgcttatc tgacatcgtt ctctgacgag gcagatgcca tcgagaaagc gaacgatacc
     2161 gaatacggcc tggccaacag cgtctggacc gccgatttgc agcgggctcg tcgggtggca
     2221 gaggccatgg aggccgccaa tagctggatc aacgcccaca acctcttccc gcacggtgtg
     2281 ccatacgccg gtattaagaa aagcgggcta ggaggggggg tactctcgga gcaaacttac
     2341 ttcgattatc tccgcagtac ctccgttgtt cgcccggtca gttgatctca ggtccagccc
     2401 cagtccgttt aggccgttgt gggtttctta gccttgttaa aatgtgccgg gtaattaaac
     2461 tagagaatga ttcgctggat gcgcaattgc ccctttcgtg aaaggggagc gatgggggcg
     2521 tcgggacctg actctggcgg atcaaaatgc gcggattatc tcggttggat gagggg
//
LOCUS       DAOPTR010000573         2569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_32002_length_2569_cov_3.197295, whole genome shotgun sequence.
ACCESSION   DAOPTR010000573 DAOPTR010000000
VERSION     DAOPTR010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32002_length_2569_cov_3.197295"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..628
                     /locus_tag="PKI05_10800"
     CDS             32..628
                     /locus_tag="PKI05_10800"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOA52733.1"
                     /translation="MSTVTTTVHDNYFMFIKLNLKLPVSPTNRDAVTWAELELRRRQA
                     RAVELFRKYVPGCEKAFIARTAPRLNIRRARCIRCDYDVTREDVLEGRHFPDEVFVYG
                     FHDMAPRYQIKDGGTYGMPYRALCVSGLDNLWCAGMLITSDYVAHMSTRNTVCSMAQG
                     QAAGTAAALCAQQKLTMRDLPYKLLRQTLEKDDVVFES"
     gene            752..2053
                     /locus_tag="PKI05_10805"
     CDS             752..2053
                     /locus_tag="PKI05_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52734.1"
                     /translation="MWTWFEQYRMDPTTWLYVASLMTIGIYFRFSRLFSIRNLDLIGI
                     IFFAPGLLLITHGLERAGYSWLFAVSALYLIRLLVDPAFARRPLMEPNLSSEGMTFMA
                     CALMFFLVANILTDRLVESQSRPETPVTESPIWRVPAHRGPGYLPFYRAITFTPQVPA
                     SRTASEGNLENLENAETSTLIVGAAIKIVAIAAQCFIVFGLILFGFRHFDNVKTGLAA
                     ASLYLLLPYTSQLTARLDHIVPGALIVWALVMYRRPMISGMLLGAATIVFYPFFLLPL
                     WIGFYQRRGLIRFLGGYLISVGTLVVLLFFISPTWHDFGNDLAAMFGKHLLRTEGADG
                     FWEYHWPYFRIPIFAGFAVLCVSLGLWPSEKNLANLLSGTAAVLLAAQFCLIHQGGLY
                     MAWYAPALILTVFRPTLEDRTAVRAVPRYWFGALPKLPGQL"
BASE COUNT          498 a          647 c          739 g          685 t
ORIGIN      
        1 agatttccgt cggtaagcga ctgaaatcgg catgtccacc gtaacgacga cggttcatga
       61 caattacttc atgttcatta agctcaattt gaagctccct gtcagtccga cgaatcgaga
      121 tgcagtgacg tgggcagagt tagaactccg ccgacggcaa gcccgtgctg tggaactctt
      181 ccggaaatac gtcccgggtt gcgaaaaggc gtttatcgcg aggaccgcac cgcggttgaa
      241 tattcggcgt gctcgttgca tccgctgcga ttacgacgta acgcgtgagg atgtgttgga
      301 agggcgtcac ttccccgacg aggtcttcgt gtatggtttt catgacatgg cgccccgcta
      361 tcaaatcaag gatggcggga cctacggaat gccatatcgt gctctttgcg tgtcgggtct
      421 cgacaatctt tggtgtgcag ggatgttgat tacgtcggat tatgttgccc acatgtccac
      481 gcggaacaca gtttgttcca tggcccaggg gcaagccgcc ggaacggccg ctgccctttg
      541 cgctcaacaa aagttaacga tgagggactt gccttataag ctgcttcggc agacactcga
      601 aaaagacgat gttgtgttcg agagctaatg acgtcccgtc atcgggtggg ttagggcaaa
      661 gcggtcaagc gggcaaaccg tggcaggttt ttcgcaagcc ggaccggctg gaggtagtca
      721 gcaatgtccc tggactagat aatctcggac tatgtggact tggtttgaac aataccgtat
      781 ggatcccacg acgtggcttt atgtcgcgtc gctaatgaca attgggatct attttcggtt
      841 ctcgcgtctt ttttccattc gcaatcttga tctgatcggc attatcttct tcgcgccagg
      901 tttattgctc atcacgcatg ggttggagcg ggccggatat tcttggctat ttgccgtgag
      961 tgccctgtat ctcatccgtc ttttggtcga tcccgccttc gcgcgtcgtc ccttgatgga
     1021 accgaactta tcgtccgaag gcatgacgtt tatggcgtgc gcccttatgt tctttctcgt
     1081 tgccaacatc ttgaccgatc ggctggtgga gtcacaatct cgtcccgaga cgccggtgac
     1141 cgaaagccct atctggcgcg ttcctgctca ccgtggtcct ggttatctgc cgttttatcg
     1201 ggccatcaca tttacgcctc aggtcccggc ttcacgcact gcatccgagg gtaacctcga
     1261 gaatcttgag aatgctgaga catcaacgtt aattgtcgga gcggccatta agattgttgc
     1321 gattgctgcc cagtgcttca ttgtgttcgg cttgatactc tttggtttcc ggcattttga
     1381 taacgtgaaa acgggcttgg cagcggcctc cctatatttg ctattgcctt acaccagcca
     1441 gctaacagcg agattggatc atattgtccc cggtgcactt atcgtctggg ccttggtgat
     1501 gtaccgtcgc cctatgattt ccgggatgct gctgggagcc gcgacgattg tcttctatcc
     1561 cttctttctt ttgccgcttt ggatcggttt ttatcaacga cgcggcctga tacgattcct
     1621 cgggggctac cttatcagtg tgggcaccct cgtagtactt ctgttcttta tctcaccgac
     1681 gtggcatgac tttggcaatg atctggcggc tatgtttgga aaacatcttt tgcgaaccga
     1741 gggagccgat ggcttttggg aatatcattg gccgtacttc cggattccta tcttcgcggg
     1801 attcgcggtt ctttgtgtga gtttgggcct ttggccgtcg gagaagaacc tggcaaacct
     1861 gctcagtgga acggccgccg tgttactggc ggctcagttc tgtttgatcc accaaggcgg
     1921 tttgtatatg gcctggtacg cccccgcgct tattttaacg gtctttcggc cgacgcttga
     1981 ggaccgaacg gcggttcgcg ccgtgccgcg ctattggttt ggcgcgttgc cgaaacttcc
     2041 aggccagctc tgaggatgag cgcaacaccg gcgtccagga tgggccacct cgtgaaacgc
     2101 agcgacgtaa catgagagag acagtttggc cgtaaatatt tttccggaac acagttagga
     2161 tcatgcgcgc attttcagta aatcggccca aaaaaccgat gggcggcttt gacttgtgtg
     2221 tccgtccttg ctacgatgat agagggtgaa aaggcagtgt gtcgtgcact catcgccgac
     2281 gcggagcttc ggcagtggta agaggtagct aagggtaggt cgccgtgcca tggaagcgcc
     2341 gacgagagtt ggaacggagg ggcgggccag acactcggtg cacatgatcg atcccaacgg
     2401 ggagatcgcg agttgggggg cctgggtgtg ttccgtgctt ggattccgga acacaagaat
     2461 aacgttcgag gtcgtcggtt tttggcaacg tttacgttgc gtgacctttt ttagaatagg
     2521 cgtatggcga tggatgtcag acttgtagtt gcgaacggcc gctattcgg
//
LOCUS       DAOPTR010000574         2564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_32087_length_2564_cov_2.845357, whole genome shotgun sequence.
ACCESSION   DAOPTR010000574 DAOPTR010000000
VERSION     DAOPTR010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32087_length_2564_cov_2.845357"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..191)
                     /locus_tag="PKI05_10810"
     CDS             complement(24..191)
                     /locus_tag="PKI05_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52735.1"
                     /translation="MKVEHRASYAAAVDKVVEYAYDYENRWVRNRLDADGDGHTDQRR
                     LLTGGACLGGI"
     gene            complement(349..421)
                     /locus_tag="PKI05_10815"
     tRNA            complement(349..421)
                     /locus_tag="PKI05_10815"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(386..388),aa:Asn,seq:gtt)
     gene            784..1686
                     /gene="argB"
                     /locus_tag="PKI05_10820"
     CDS             784..1686
                     /gene="argB"
                     /locus_tag="PKI05_10820"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007335411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOA52736.1"
                     /translation="MQRAIEKADVLIEALRWIRTFRGKITVIKLGGSVMEDERAMSHL
                     LLDILFMETVGMQPVVVHGGGAAISRAMKKAGIQPRFVQGRRYTDPETLEIVEQVLAY
                     EINEKLAQEIEKLGGRAQPLNFRTKNVLFGKKLVLTDESGNPIDLGLVGEVTRVDSDV
                     ILDLCTRGVVPVIPSMCLDEEGTKLNVNADTAATAVARAVRAEKLVFLSDVNGVRLRK
                     DDPDSLIHSLTAAQARELIRQGVIEEGMIPKVEACLETLEHGVNKIHIIDGRLRHSLL
                     LEVYTTTGVGTEIIRDGSARNNNG"
     gene            1784..>2564
                     /locus_tag="PKI05_10825"
     CDS             1784..>2564
                     /locus_tag="PKI05_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOA52737.1"
                     /translation="MTHSSRSSAEIIALFDRYVIGNYRRFPVALVRGEGVYVWDAEGK
                     RYLDFFPGWGCNLLGHCPPRVVEAVREQVARLIHVPNTWYTELQGLWAQALSERSFGG
                     KAFFCNSGTEANEAAIKLVRLHSPKGRYKIITFEGGFHGRTMGSLSATAQPKYHEGLG
                     PLLPGFVYCPFGDLDEVAKRIDNETAAIMIEPIQGEGGVVVPPEGFLVGLRKLADEHG
                     LLLVFDEVQTGCGRTGHWFGYQYFGVTPDIMTLAKAVCGGLP"
BASE COUNT          510 a          644 c          833 g          577 t
ORIGIN      
        1 ctctcccaaa gggagagggg agtttatatg cctcccaggc aagcgccccc ggttaacagc
       61 cgccgctggt cggtatgccc gtcgccgtcg gcatccagcc ggttgcgcac ccagcggttc
      121 tcgtagtcgt aggcatactc caccaccttg tccaccgccg cggcgtagct tgcgcggtgt
      181 tcgaccttca ccaggcggtt gcggtggtcc caggtgtact gggtgatgtc ggtatcgccg
      241 ctgtcgagct ggccgttctg gttggcgtcg atgagccggt gcgtgcggtt ccgctcggcg
      301 ttttctggaa accggtgtgc ggcgtttatt cggggcagaa cagaaaaact ccccggccag
      361 ggctcgaacc tgggacctag cggttaacag ccgctcgctc tacctgctga gctaccgggg
      421 aatgagtggc gaacgaatca ccaaagcgtt ggcaaaccta acccgttcgg gtaggccgtg
      481 gggggctacc aacagcgccc catcaggtgg ccaactttca ctagcaagac ttcattcatg
      541 ttatctgcct tgttgaaatc aatcaaggtg ggtcgaactt ggagcaaacg agtctcgaaa
      601 aaccgaaccc cattgacggg gaatagccaa gtgcgaaaat aaaagttttc gatctatgat
      661 tgcggaggcg aaccgggcgc gaacgcacgg gcgggcccgg tgtcgttgtt ttggggggat
      721 ttggatacag ttgccgttgt caatgtaagc gtcgtttttt gagagtgacc gggggcccaa
      781 gcgttgcaga gagcgatcga aaaagctgac gtcttgatcg aggccttacg ctggatccgc
      841 accttccgcg gaaagatcac cgtcatcaaa ttgggcggca gtgtgatgga ggacgagcgg
      901 gcgatgagcc atctcctgct cgacatcctc tttatggaga ccgtcgggat gcagccggtg
      961 gtcgtccatg gtggtggggc ggctatcagt cgcgcgatga aaaaggccgg aatccagcct
     1021 cgttttgttc agggacgtcg ttacacggat ccggagactc ttgagatcgt tgaacaggta
     1081 ctggcctacg aaatcaacga aaaactcgct caggagattg agaaactggg tgggcgggcc
     1141 cagcccctca atttccggac gaagaacgtc ctgtttggga aaaagctagt cttgacggac
     1201 gaatcgggta atcccattga tctggggttg gtgggcgagg tcacccgggt tgatagcgac
     1261 gtcattctag atctgtgtac gcggggtgtc gtgcccgtca ttccttccat gtgtttggat
     1321 gaagagggga cgaagctcaa cgtcaatgcc gatacggcgg caacggcggt ggcgcgggcc
     1381 gtgcgtgcag agaaattggt ctttcttagt gatgtgaatg gtgttcgtct gcgaaaggat
     1441 gatccggact cgctaattca ctccctcacg gctgcgcagg ctcgggagtt aatccgccag
     1501 ggggtgattg aagagggaat gattcccaag gtggaggcgt gtttggagac gctcgagcac
     1561 ggcgtgaaca agatccacat tattgacgga cggcttcgcc attcactctt gttggaagtt
     1621 tacacgacca cgggggtggg gacggagatc attcgcgacg gatcagcccg caataacaac
     1681 ggttagccca tcccgccgca tcttgcagca tcctggcaga gagccaccaa cacttggatt
     1741 gtttggggat ttggtctttt gggagaacag aggaaggccc acagtgactc acagtagtcg
     1801 ttcgtcggcg gagattattg ccctttttga tcgctacgtc atcggcaact accgtcgttt
     1861 tccggtagcc ctggtgagag gcgagggtgt ttacgtttgg gacgcggagg ggaaacggta
     1921 tctggacttt ttccctggct ggggctgtaa cttgttggga cattgtcctc cgcgggtggt
     1981 cgaggctgtc cgcgagcagg tggcacggct catccacgtg cctaacacct ggtacacgga
     2041 gttgcagggg ctgtgggccc aggccctttc cgagcggagt tttggtggaa aggcgttttt
     2101 ctgcaattca ggtaccgagg ccaacgaggc ggcgatcaaa cttgttcgcc tgcacagtcc
     2161 caagggacgc tataagatca tcacgtttga aggtggcttt catgggcgga caatgggctc
     2221 gttgtcggcc acggcccagc ccaagtatca cgagggattg gggccgcttt tgccgggttt
     2281 tgtttactgt ccctttgggg atttggacga ggtggccaag aggattgaca acgaaacggc
     2341 cgccatcatg atcgagccca ttcagggtga gggcggtgtg gttgtcccgc ctgaggggtt
     2401 tttggtgggc ttgcgaaaac tggcggacga gcatgggttg ttgctagtct ttgatgaggt
     2461 acagaccggc tgtggcagaa caggccattg gttcggctac cagtacttcg gtgttacgcc
     2521 cgacatcatg acgctggcca aggcggtatg cggtggcctg cccg
//
LOCUS       DAOPTR010000575         2559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_32154_length_2559_cov_3.498003, whole genome shotgun sequence.
ACCESSION   DAOPTR010000575 DAOPTR010000000
VERSION     DAOPTR010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32154_length_2559_cov_3.498003"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            397..1317
                     /locus_tag="PKI05_10830"
     CDS             397..1317
                     /locus_tag="PKI05_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52738.1"
                     /translation="MDSETPASSPLGAILLVAETRAQGSLRERLRERRETRSLARNRE
                     KDPLVPQEPNDWQVGRRITRERVQTWIEQLRDRIRAEIGDSDGARDLEIPRVPGGRTD
                     SALPSTVAPPGNTWPPRPLVPTDQQLSAGVKDSSSSQLQQPEVIGPELVPPESPSAPP
                     SAMKNAHPDSAVPFSTIESPPPTSLPGTTEKPVEDNRNQDSTGLDPGGEGVTSQPGAT
                     AASKPTGLSGLFSNVAQMFGTATSPRVEKPSKDESQENQENQAGSNQTADPQNVPASS
                     KPRSTTGKTLAEIRKQILLKQSSKSAAPRQ"
     gene            complement(1409..2167)
                     /locus_tag="PKI05_10835"
     CDS             complement(1409..2167)
                     /locus_tag="PKI05_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOA52739.1"
                     /translation="MACTQHRDAVALVTGAGKRRVGSFIVRMLADRGYKVAIHYRSSQ
                     QEAESLKHELVSQGKQAEIFQADMADTASIDQMFERVLGHFGRLDVLVTAAAIWEPTR
                     FETLTPESVMEHFRVNALGAFWCCRRAGLAMVGQPEGGVIITIGDWAIERPYLNYASY
                     FRSKGCIPTMTRVLAIELSHRNPNIRVNAIMPGPVMMPSHLPEEEKVEAIRSTLLQKE
                     GKPEHIVSAVEFLIDNDYVTGVCLPVDGGRTIGG"
     gene            complement(2306..>2559)
                     /locus_tag="PKI05_10840"
     CDS             complement(2306..>2559)
                     /locus_tag="PKI05_10840"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOA52740.1"
                     /translation="VACGAAGELLDDARACGCDVIVLGEARYHTCLEAEFWGIGMVLV
                     GHYASERRGVEKLAEILRRHFPHLKVWASEQESDPMCWV"
BASE COUNT          605 a          759 c          674 g          521 t
ORIGIN      
        1 cgggatcaga gcttgaatcc gagattcgac gtttgctcca ataacttgaa cgatcgtcta
       61 gaatgacggc acggctttgt attaaccgtg ggtatcgtta gggaccacaa agacaggctc
      121 aacacagcct gtctttgctc gttgaaatgt ggtaggttgg cagagtacga tcaccgcctg
      181 ggcggaaaga ctattgtcgt gctgttggtg agcgaaacat cccggggcgg agatgagacc
      241 atgggtttta tgagtaacgt attacgccac agttcgggtg ttcttcggtc acgcctaata
      301 ctacttctgt gggtaacatc agtttggatt ttgcccatcc ccgtggcgga gtctcgagaa
      361 aggcatgccg attctagaag gatagcggtg aacgccgtgg acagcgaaac acctgcgtct
      421 tccccgctgg gtgcgattct tctggtcgct gaaacaaggg cgcaaggtag tctccgtgag
      481 cgtctccggg aacgacggga aacacgttct cttgcaagga accgcgagaa ggacccattg
      541 gtccctcaag aaccgaacga ttggcaggtc ggccgccgga taacccgcga gcgagttcaa
      601 acatggattg aacagttgcg cgacagaatt cgggcagaaa tcggcgattc cgacggtgcc
      661 cgcgatctgg aaatcccacg ggtccctggt gggagaaccg attcggcact cccaagtacc
      721 gtggcaccgc ccgggaacac ctggccgcct cgtccgcttg tgccgacaga tcagcaactg
      781 tcggcaggtg taaaggactc gtccagtagt caactgcagc aaccggaagt aatcggacct
      841 gagctcgtcc caccggaatc gccctccgcc ccaccctccg cgatgaaaaa tgcgcacccg
      901 gattctgccg ttccgtttag cacaatcgag tcccctccgc caacaagctt gccgggaact
      961 accgaaaagc cggtggaaga caatcgcaac caggacagca ccggcttaga tcccggtggt
     1021 gagggggtca cttcgcaacc cggtgcgact gctgcgagca aaccaactgg tctcagcggg
     1081 ctgttctcca atgtggcaca gatgtttgga accgccacct cgcccagggt tgaaaagccc
     1141 agcaaagacg aatcccagga gaatcaagag aatcaagccg gctctaatca aacggccgac
     1201 cctcagaacg tgcccgcgag ctcaaaacct cggtccacca cgggaaaaac gcttgccgaa
     1261 atccgaaagc agatactctt gaaacaaagt tcaaagtctg ctgccccccg ccaatgagtg
     1321 gcccgtgatc aacgtgacac aaggtaccac agtcgctcca cgggggcaaa cactgtgtct
     1381 ttgttttcgt ccggcagcag catttgtctc agccaccaat ggtccggcct ccatcgacgg
     1441 gaagacaaac gccggtgacg taatcgttgt caataaggaa ctccactgcc gaaacgatgt
     1501 gctccggttt tccttctttt tgcagcagcg tgctgcgaat ggcctcgact ttctcttcct
     1561 ctggcaaatg gcttggcatc atgacgggcc ccggcataat agcgttgacg cgaatgttgg
     1621 gattccggtg actaagctca atcgctaaga cccgcgtcat ggtgggaata caacccttcg
     1681 agcgaaaata gctcgcatag ttcagatacg gtctctcgat ggcccaatcg ccaattgtga
     1741 tgataacgcc gccttcgggc tggccgacca tggccaaacc cgcacggcga caacaccaaa
     1801 acgcccccaa tgcgttgacc cgaaagtgct ccatcacgct ttcgggcgtt agcgtctcaa
     1861 aacgcgtcgg ctcccagatc gctgctgccg taaccaggac atcgagccgc ccaaaatgcc
     1921 caagtacccg ctcgaacatc tgatcaatgg aggcggtatc cgccatgtcg gcctgaaaga
     1981 tttcggcctg cttgccttgg gataccaact catgcttgag cgattctgcc tcctgctgcg
     2041 agctgcggta atgaatagca accttgtatc cccggtcagc gagcatcctc acaatgaagc
     2101 tgccaacgcg tcgctttcca gcgccggtta ccagggcgac tgcatcacga tgttgagtac
     2161 aagccatacg gtaccactcc tagctattcc tagccgacgc ggggtgtcgt gattaatttt
     2221 attcctaatt gcggattctt ctcagtgagt accttcttac cactgcctta agagtggcaa
     2281 gaaaatttta taaatcagtt agcacttaaa cccagcacat cggatcggac tcttgctcgg
     2341 aggcccaaac cttgaggtgc gggaaatgcc ggcggagaat ctcagccagt ttctctacgc
     2401 cacgcctttc gctagcatag tggccgacca agaccatacc gatcccccag aactccgctt
     2461 ctaggcaggt atgatatctt gcctcgccga gcacaatcac gtcgcaaccg cacgcccgag
     2521 cgtcgtccag caactcgccg gcggcaccgc acgccacag
//
LOCUS       DAOPTR010000576         2558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_32168_length_2558_cov_3.729924, whole genome shotgun sequence.
ACCESSION   DAOPTR010000576 DAOPTR010000000
VERSION     DAOPTR010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32168_length_2558_cov_3.729924"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..738
                     /locus_tag="PKI05_10845"
     CDS             109..738
                     /locus_tag="PKI05_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HOA52741.1"
                     /translation="MAQPERPTGDAQPACCASRKASDLQPGGEGGSHLPRRGILGSVV
                     AWVLGLFALSPSFVAGIAAFLSPLGGKAQKGVLLRLTPLDNLPKDGTPRKFPVIAEKR
                     NAWTFSREPVGAVFLRLLPNGDVHALHVVCPHAGCAIEYRPAQGGGQEGQFVCPCHLA
                     HFDLSGKRLDAVSPSPRDMDTLRVEVRDGEVWVEFQNFQLGTADKKPVA"
     gene            1038..>2558
                     /locus_tag="PKI05_10850"
     CDS             1038..>2558
                     /locus_tag="PKI05_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HOA52742.1"
                     /translation="MTFGDWLDDRIGWRGIWRASCGGGCDARGRCWWPISLSVIFFLL
                     VQQSLTGFFLWIYYSPSSQTAWESVYFIQHHVTLGWLLRGIHFWGAQVLVGFLAISLA
                     LRIFSGFYRAPREWVFWTGLVLFAFALGSCLTGDLLRWDQEGYAATQTRVSFLMLLPK
                     VGASLYRLAAGGPEFGHHTLTRFFALHVGVLGFGMWLFALAHAALAGRASRAVETSPG
                     TYKNARPDPRFPVVVQGLVCLLALVVVLLFVFHRGLPGIGEVSAWKSPQTSFGAALGA
                     PADLDPAHFYGAARPEWSFRGLYGFSNVFPGELKILPIFVIPGIIAVLVLAMPILGRC
                     SFGHYWNMLLTVALIGGLGYFTYTSYAHDKHDEGYQAAKTAADEEAARTVELIALRGG
                     VPPAGALALLRQDPKTEGPRVFEQQCVSCHNFSTTEGKPLLGDAPSAPDLFGFASREW
                     LKGFLDPKQIVTEKYYGNTRFAAGAMVRYVEERFAKLPEEEQAAIIAALSAEARLPQ"
BASE COUNT          492 a          657 c          776 g          633 t
ORIGIN      
        1 ttcgctgagg acattatttc tgtctgcaaa ccattccccg gcaaagatcg tatctgaaca
       61 gtcgggataa taggctcgcg tttgaagaat tggagcggag gtcattgtat ggctcagcct
      121 gaacgcccaa caggtgacgc tcaaccagca tgttgtgcat ctcgcaaggc ttccgacctg
      181 caacccggtg gcgagggagg atcgcatcta ccgcggcgcg ggatactggg gagcgttgtg
      241 gcgtgggttt tggggctatt tgcactaagt ccctcttttg ttgccgggat tgctgcgttt
      301 ctttcacccc ttggcgggaa ggcacaaaaa ggggtcttgc tgcgtttgac gcccctggac
      361 aatctcccta aagatggaac gccgcggaag ttccccgtca ttgcggaaaa acgtaatgcg
      421 tggacgtttt cacgtgagcc agttggggcc gtcttcctcc gcctcctccc gaacggtgac
      481 gtccatgcct tgcacgtggt gtgcccccat gcgggatgtg ccattgaata tcggccagca
      541 caaggcggtg gccaagaagg gcaatttgtg tgtccgtgcc atttggcaca ttttgatttg
      601 tcgggcaagc ggcttgatgc agtatcgccc agcccacgag acatggacac acttcgggtg
      661 gaagtccgtg atggcgaagt gtgggtggaa tttcagaact tccaattggg cactgcggat
      721 aagaaacccg tagcgtgatg cttttgccct ggatatcttg gtcagcaaat ccggatcaat
      781 atagacaaga gctgaaagag gaataagagt ctatcccaaa aagtctccct ctcgctctgg
      841 gagagggttg gggtgagggc aagcgatcat cggagttaat cgagaaaacc ggcggagatt
      901 cgagccagcc ttacccccga ctcctctccc gccgaacggg gcgaggggag attatggggt
      961 aggcactaag cggagacaat cacgtcataa ggcaagtttc tcgacggcat gagcacttgt
     1021 aatccaaggg taccgatatg acgtttggcg attggttaga tgatcgaata gggtggcgag
     1081 ggatttggcg ggcaagttgc ggagggggct gcgatgccag gggacgttgc tggtggccaa
     1141 taagtctttc ggttatcttc tttcttctcg tgcagcagtc cctgacgggt ttcttcttgt
     1201 ggatttatta cagtccctca tcgcaaactg cctgggaaag cgtgtatttt attcagcacc
     1261 acgttacgtt gggctggttg ctgcgcggaa ttcacttttg gggggcccag gtcttggtag
     1321 gctttctggc gatttcgttg gccctgcgca tcttctcggg attctatcgg gccccgcgag
     1381 agtgggtatt ctggacggga ttggtcctct ttgcgttcgc tctaggatcc tgtctgacgg
     1441 gcgatcttct ccgttgggat caagagggat atgccgccac gcaaacacgg gtcagctttc
     1501 ttatgctgct ccccaaggtc ggggcgagcc tttatcggtt agccgcaggt ggacctgaat
     1561 tcggtcatca tacgctgaca cggtttttcg ccctccatgt cggtgtgctc ggatttggta
     1621 tgtggctatt tgccttggcg cacgcagcac tggcaggacg ggcaagccgt gcggtggaaa
     1681 catcgcctgg cacctataaa aacgctcggc cggacccccg gtttcccgtc gttgtgcaag
     1741 gattggtttg tttattggcc ctcgtcgttg tcctcttgtt tgttttccat cgtggtcttc
     1801 ctggcatcgg cgaggtctct gcctggaaat ctccccagac gagttttggg gcggccctgg
     1861 gggcacctgc cgatctcgat ccggctcact tctatggagc agcccgaccc gagtggtcgt
     1921 ttcgtggctt gtatggtttc tccaacgtat ttcctggtga actaaagatt ctcccgattt
     1981 ttgtgattcc cgggatcatt gctgtattgg tacttgcaat gcccatcctc gggaggtgct
     2041 cattcggcca ctactggaat atgctgctca cagtagcgct aatcggtggc ttggggtatt
     2101 tcacttatac ctcctacgcc catgataagc atgacgaggg ctaccaagca gcgaagacag
     2161 ccgctgatga agaggccgcg cgaactgtgg aattgattgc tctgcgggga ggtgtacccc
     2221 cggcgggggc gttggccctg ttgcgacagg accccaagac agaaggtccg cgcgtgttcg
     2281 agcagcaatg tgtcagttgc cacaacttct cgaccacgga ggggaaaccg ttactcgggg
     2341 atgccccttc ggccccggac ctatttggtt ttgcctcccg agaatggctt aaggggttcc
     2401 ttgatcccaa gcagatcgtg acggagaaat actacggaaa tacgcgtttc gcggcgggtg
     2461 ccatggtccg ctatgtcgag gagcgattcg caaagctacc tgaggaggag caagcggcca
     2521 tcattgcggc cctttcggcg gaggcacggc tgccccag
//
LOCUS       DAOPTR010000577         2554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_32229_length_2554_cov_4.019208, whole genome shotgun sequence.
ACCESSION   DAOPTR010000577 DAOPTR010000000
VERSION     DAOPTR010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32229_length_2554_cov_4.019208"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="PKI05_10855"
     CDS             <1..1067
                     /locus_tag="PKI05_10855"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOA52743.1"
                     /translation="RRFEEEFAQAVECRYAVAVANGTVALEAALEALGIGPGDEVVVT
                     CRSFVASAACVLRVGATPVFADVDRDSQNITAETIAPVLTPRTRAVIAVHLAGWPCDM
                     DPIMELASRAGLAVIEDCAQAHGARYKGQPVGSLGHIAAFSFCQDKIITTGGEGGMVT
                     TNDPQLWRKVWELKDHGKNWDLIRQPGNGTFRWVHESLGTNWRMTEMQAAIGRVALTK
                     LENWVGIRRANAAILRRRLAHLPALRIPSPPPTVEHSYYKFYAFLRPEFLKPGWDRDR
                     ILAAIQAEGIPCGAGVCPEIYREKLFQDRALGPAAPLPVAHELGQTSLMFLIHPTLEE
                     RDMIDTATAIEKVLTAATVG"
     gene            complement(1258..>2554)
                     /locus_tag="PKI05_10860"
     CDS             complement(1258..>2554)
                     /locus_tag="PKI05_10860"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4976 domain-containing protein"
                     /protein_id="HOA52744.1"
                     /translation="ESAEVPVNLDYFRCISGVDDCVGRILAGLEEYGVAEDTLIIFQS
                     DNGFYLGEHGLGDKRSAYDESLRIPLLARYPKAIVKGLVRDEMVLNLDLAPTILDFAG
                     LPIPSQMQGQSWRPLAEGKQVPWRKAWLYEYFAEKQRNSRVPDIVAVRTESAKLITYP
                     GHDDWTELFDLKNDPYETRNLYRDPAAATLRAAMEAELERLKKDVDYRVPDYVDRPDW
                     WGGSPPDPNAPPEVCLDYQLGEAGQGDTIVDRSGRGNNGVNRGTVVRQSSDGQTVRYF
                     DGNGLIEVPNSPTLNPADMSWTVEVVFRPEAADGVLVARGGQSQGYALWLREGRPVWT
                     VVVSNQVISVESAEQVTGWATLTGVITPQRQAALYVNGTLVGRKPLPDFISTDPRDSM
                     QIGADTGSPIVPAGTQRFKGEMKRVTIYRGARPPQTTGQ"
BASE COUNT          489 a          814 c          722 g          529 t
ORIGIN      
        1 cccgccgttt tgaagaggag tttgcccaag ctgtggaatg ccgctatgcc gtggcggtgg
       61 ccaacggcac ggtcgccctg gaggccgccc tggaggcctt ggggatcggt ccgggagacg
      121 aagtggtcgt cacctgccgg agttttgtgg cctctgccgc ttgcgtgctc cgggtgggtg
      181 ccacgcccgt ttttgcggac gtggatcgcg acagtcagaa catcacggcc gaaacgattg
      241 cccccgttct cacgccccgg acccgcgcgg tcattgccgt tcacctagca ggttggccat
      301 gcgatatgga ccccattatg gaattggcct cccgcgcggg actggccgtc atcgaggatt
      361 gcgcccaggc gcatggggcg cgttataagg gccagcccgt tggctcactg ggccacattg
      421 cggccttctc attttgccag gacaaaatca tcaccaccgg gggcgaaggc ggcatggtca
      481 ccacaaatga tccccaactg tggcgcaaag tatgggagct caaagatcat ggcaaaaact
      541 gggatttgat acgacagccg gggaatggga catttcgctg ggttcacgag tctttaggca
      601 cgaattggcg aatgaccgag atgcaggccg ccatcggccg ggtggccttg accaagctgg
      661 aaaactgggt cggtatccgt cgtgccaacg ccgcaatact tcggcgccgc cttgcccatc
      721 tgccggcgct gcggattccc agcccaccgc ccaccgttga gcacagctat tacaagtttt
      781 acgccttcct taggcctgaa ttcctcaagc caggctggga tcgtgaccgc atcctggccg
      841 caattcaagc cgagggcatc ccttgtgggg ccggtgtttg cccagaaatc taccgcgaaa
      901 agctcttcca agaccgcgcc cttggccccg ccgcgccgct tcctgtggcc cacgagctgg
      961 gccaaaccag cctgatgttc ctcattcatc ccacactgga agaacgcgac atgatcgaca
     1021 cagcgaccgc catcgaaaaa gttctaaccg cggccaccgt cgggtaacca cgctggatta
     1081 gtttagagcc tatcccacaa cctcccctcg cccgtttggc gggagaggga tcgggggtga
     1141 gggtgactcg aatcgctgcc ggttttctcg ctcgactccg acgatccctt gccctcaccc
     1201 taaccctctc ccggaggcag atggggactt tttggaatag gctctcaatg ccgatcctta
     1261 ctggccggtt gtttgtggcg ggcgtgcccc ccgataaatc gtcacacgtt tcatctctcc
     1321 cttgaatcgc tgggttccgg cgggaacgat cgggctgccc gtatccgccc caatctgcat
     1381 gctgtcgcgg ggatcggtcg agatgaaatc gggcagcggc tttctcccta ctaatgttcc
     1441 gttgacataa agcgccgcct gtcgctgtgg tgtgatcacc ccggtcagcg tcgcccaacc
     1501 ggtaacttgt tccgcggact ccaccgagat aacttgattg ctgacaacca cggtccagac
     1561 cggtcgccct tcgcgcaacc aaagggcgta gccctgggac tgaccgcctc gggcaacgag
     1621 caccccatcc gccgcttccg gacggaagac cacctcgacc gtccacgaca tatcggccgg
     1681 gtttagcgtt ggcgagttgg gcacctcgat caggccgttc ccatcgaaat atcgcacggt
     1741 ttgaccgtcg ctcgactgac ggacaaccgt gccacggtta accccgttat tgccccggcc
     1801 tgaacgatcg acaattgtat cgccttggcc ggcttcaccc agttggtaat ccaggcaaac
     1861 ttccggcggg gcattggggt ccggcggtga accaccccac caatcgggac gatcgacgta
     1921 atcgggaacc cgatagtcaa cgtccttctt aagtcgctct aactccgctt ccatggccgc
     1981 ccgcaatgtg gcggcagccg ggtcgcggta aagatttcgg gtttcatagg gatcgttttt
     2041 caggtcaaac agctccgtcc agtcgtcgtg acccggatag gtaatcagct tggccgactc
     2101 tgttcgaacg gcaacgatgt cgggaacccg gctatttcgc tgtttctccg caaaatattc
     2161 gtagagccat gcctttcgcc acggcacctg tttcccctcc gcgagtgggc gccagctttg
     2221 tccctgcatc tgactgggga tggggaggcc agcaaaatcc agaatcgtgg gcgccaggtc
     2281 aaggttcaga accatttcat cccgcactag gcccttcaca atggctttgg ggtagcgggc
     2341 caagaggggg atgcgcaggc tttcgtcgta cgcgcttcgc ttgtctccga ggccatgttc
     2401 gcccagataa aacccgttgt cgctctggaa gatgatcaac gtgtcctccg ccaccccgta
     2461 ttcttccaaa ccagccagaa tccgcccgac acagtcatca accccgctga tacaccggaa
     2521 atagtcgaga ttaactggca cctccgccga ttcc
//
LOCUS       DAOPTR010000578         2554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_32237_length_2554_cov_3.323729, whole genome shotgun sequence.
ACCESSION   DAOPTR010000578 DAOPTR010000000
VERSION     DAOPTR010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32237_length_2554_cov_3.323729"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            320..1612
                     /locus_tag="PKI05_10865"
     CDS             320..1612
                     /locus_tag="PKI05_10865"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOA52745.1"
                     /translation="MSAPDPQKQREFAVWVVRKLQERGYIAYWAGGCVRDQLLGRVPK
                     DYDVATNATPDEVRNIFGMKRTFAVGAAFGVITVNGPPGAGQIEVATFRTDSSYRDGR
                     HPESVSFSSPKEDALRRDFTINGLFYDPIQDRTVDYVGGVEDIKRRIIRAIGDPYQRF
                     TEDKLRMLRAIRFVAELEFDLDPRTYEAIRAMADQITQVSQERILMELERLLTAPARR
                     KGVILLRESGLLRVVLPELEPRDNAQEQQWMTNLDILAKLKEPGFPLAFAVVARGLVE
                     PQHVTSLCRRLRMSNQQSDKIQWLLEHERSLEGAEKKRWSEVQPILISPHIDDLLNWM
                     ESRSEVENRPSNSALWCRERLNWPKEQLNPPPLIRGDDLLETGMVQGPMFREILDRVR
                     KAQLDGIVTTREEALQFGLGLAKNYDLNKNTQRSGNGR"
     gene            1660..1854
                     /locus_tag="PKI05_10870"
     CDS             1660..1854
                     /locus_tag="PKI05_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52746.1"
                     /translation="MTEKRLDRRDKRRLESLRAQLQDLQRRLAGAKKQPDDPREIQQL
                     EQQIKAVQAEIDRITSQGSR"
     gene            2258..>2554
                     /locus_tag="PKI05_10875"
     CDS             2258..>2554
                     /locus_tag="PKI05_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOA52747.1"
                     /translation="MNGKSTEFVKIASLRSIAEFQEHIASLGYELPVDEQILSAAESS
                     PLAQPIEVGGYRIGNRWCVHPMEGWDGTPDGQPSKHTIRRWEHFGQSGAKLIWGG"
BASE COUNT          614 a          666 c          741 g          533 t
ORIGIN      
        1 agccatgcgc gtctgccagt tccgccacca gggcaatcgg caaaaaccta ctctgttctc
       61 gctcagatcg ggcacgcgat cttaggacgt gcctcatcga tcgccgacaa aagctcatcg
      121 cgccgttgcg gcacccattg tgatttttgc taccgcaacc aacaatcgca aatagcaaat
      181 gtattttaca cgggcaacgc agagacggga agtcaacccg actgccaacg cggcctttgg
      241 cctgacccgg gggttttatc cgcgtgttac ccggctagcc ggttttctga gaccgttaat
      301 ttttctagcg agagcattca tgtcggctcc ggatccccaa aaacaacgtg aatttgccgt
      361 ctgggtggtg cggaaactcc aggaacgggg ttacatcgcg tattgggcag gtggttgtgt
      421 ccgcgatcaa ttgttaggac gggttcccaa agattatgac gtggcaacca atgctactcc
      481 cgacgaggtc cgcaatatct ttgggatgaa gcgaaccttt gccgtgggtg ccgcgtttgg
      541 ggtgattacg gtcaacggtc cccccggggc ggggcaaatc gaggtcgcca cttttcgcac
      601 tgacagcagc taccgggacg gccggcatcc ggaaagcgtg tccttttcct cgcccaagga
      661 agatgctctc cgccgagatt tcactatcaa cgggctcttc tacgatccta tccaggatcg
      721 gacggtcgat tatgttggcg gggtcgaaga catcaagcgt cgaatcatta gggccatcgg
      781 cgatccctac cagagattca cagaagacaa gcttaggatg cttcgcgcca ttcgctttgt
      841 agcagagctc gagttcgacc ttgacccgcg cacctacgag gccattcgtg ctatggcaga
      901 ccaaatcacg caggtcagcc aggaacggat actcatggag cttgaacggc tactcacggc
      961 cccggcacgc cggaaaggcg tgatactcct gcgggagtca ggcctattgc gtgtggtctt
     1021 gccagaactc gaaccgcggg acaatgccca ggaacagcaa tggatgacga atcttgacat
     1081 cctcgccaag ttgaaggaac ccggttttcc cttggctttt gccgtggtgg cgcggggatt
     1141 ggtcgaaccg cagcacgtca catcgttgtg ccgccgctta aggatgtcga atcagcaaag
     1201 tgacaaaatc cagtggcttc ttgagcacga acgatcattg gagggggcag aaaaaaagcg
     1261 atggtccgag gtccagccga tcctgatatc gccgcacatc gacgatttgc ttaattggat
     1321 ggaatcgcgt tcggaggtcg aaaaccggcc gtccaatagc gcgctgtggt gtcgtgaaag
     1381 attaaattgg ccaaaggagc aattgaatcc gccgcctctc atccgtggcg atgatctgtt
     1441 ggaaacaggt atggttcagg ggccaatgtt tcgggaaatt cttgaccgcg ttcgtaaggc
     1501 ccaactggat ggcatcgtta caactcgtga agaggcctta caatttggcc ttgggctggc
     1561 caaaaactac gacctcaaca aaaatactca gcggtccggg aacggccgat aagcacatcg
     1621 accaccagtt tacaactgga aaacgtggag gatgaaatcg tgacggagaa aaggctcgat
     1681 cgacgtgata aacgccgttt ggaatccctg cgggcacagc ttcaagactt acagcgtcga
     1741 ctagcaggtg ccaaaaaaca accggatgat cctcgggaaa ttcagcagtt ggaacaacag
     1801 atcaaagccg tccaggcgga gatcgaccgt atcacttcgc aggggtcccg gtagggtggt
     1861 tgcgtagccc cgcccgttcc gaagatcgaa acacgagcgg agcaatttgg cctacagtgt
     1921 ggaaaaaggg tcggcggtgt gggacgatct tccggaagta cccgcgaata agtgaatctc
     1981 gagagtaccg tctgagccct gagctttgtc atgggagcaa tcgctacgaa agataacccg
     2041 caaggcaacc cagcgcgata ggtgcactcc acagaatttg caaagataat agcgtgagaa
     2101 gttggacagc ttgggctgat aaatggacaa ggtctgcggc caaaacgatc tttggccctt
     2161 ggataccggt atttgcaccg aggttgcctg ctttcgtgga aacgagatag ggcgttgacc
     2221 gagcagttgc acagtccgac ttgaataagg atgagcgatg aacggaaaat cgaccgagtt
     2281 tgtcaaaatt gcctcactgc gatccattgc ggagtttcaa gagcacatag cgtctttggg
     2341 gtatgagctt ccggtggatg agcagatcct atcggcggct gagtcatccc cgcttgcgca
     2401 gcccatcgaa gttggcgggt atcgcatcgg aaaccgctgg tgcgtccatc ctatggaagg
     2461 ttgggacggc acgcccgatg gtcagccgtc caagcacacc atacggcggt gggagcattt
     2521 cggccaatcg ggggcgaagc taatctgggg tggc
//
LOCUS       DAOPTR010000579         2554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_32240_length_2554_cov_3.118047, whole genome shotgun sequence.
ACCESSION   DAOPTR010000579 DAOPTR010000000
VERSION     DAOPTR010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32240_length_2554_cov_3.118047"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1939)
                     /gene="feoB"
                     /locus_tag="PKI05_10880"
     CDS             complement(<1..1939)
                     /gene="feoB"
                     /locus_tag="PKI05_10880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOA52748.1"
                     /translation="MKRGNSCNTCPNCVTKPTLRLRRLGVEAGSADFVVALAGNPNTG
                     KSTVFNALTGLHQHTGNWAGKTVALAEGSFEFGSRRFKIVDLPGTYSLLATSYDEEVA
                     RNFLLFGQPDVTIVVVDATRLERNLNLALQVLEITHRVILCVNLIDEAERHGLVIDTE
                     LLAAELGIPVVATSARFGRGIDTLLKAIEGVATGEIQCHPLRVGGSTPQLSEAVQDLT
                     KELERAFPHLTNLGWIAIRLLDGDERIVKAIRTGELAQLHRQNHEAVSRDSATENRPP
                     SLDEGPSEDSRERILAKASAWRWQIGPNAHQTLVEAIYAHAGRIADRAVRRKEGVSST
                     WDEKLDSILTSPIWGYPTMIALFVVVIWITVQGANYPSQVLSWFLVETLHPLLRSAAV
                     GVHLPNWLIGLFLDGGYLATAWVISVMLPPMAIFFPLFTLLEDLGYLPRVAFNLDRLF
                     RWAGGHGKQALTMSMGFGCNAAGVVACRIIDSPRERLVAILTNNFSLCNGRWPTQILL
                     ATVFLGSLVPSYLAGFVAAGGVIAVALLGVVMALVTSRLLTQTVLKGEPSTFHLELPP
                     YRPPQILHTLYTSLLTRTLVVLWRAVVFAFPAGLVIWLIANVHIGSQPLAEYLVRFLD
                     PIGLAVGLNGIILLAYVVAIPA"
     gene            complement(1958..>2554)
                     /locus_tag="PKI05_10885"
     CDS             complement(1958..>2554)
                     /locus_tag="PKI05_10885"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA domain-containing protein"
                     /protein_id="HOA52749.1"
                     /translation="VAQLGDPRYDPHGDPIPTDSGETQGRLGKPLTSMSPGEFVEVTH
                     MEDEPASAYRELIDRGLHLGARLQVETLAPTEIQVRHHGETVVLSPQAAANLNVKAIP
                     RYEFAQWEKKPSTLADLRLGEEAVVVGIAPLCRGLVRRRLLDLGFVPGTKVRAELSSL
                     GGDPTAFRVRSTLIALRKSQAASILVKRENETSPTPST"
BASE COUNT          632 a          722 c          675 g          525 t
ORIGIN      
        1 tggcaggaat tgcaaccacg taggccagca aaataattcc gttgagcccc actgccagac
       61 cgatgggatc aaggaaacga acaagatact cagccagggg ctgcgaccca atatgaacat
      121 tggcgatgag ccaaatgacc aggcctgccg ggaaggcgaa cacgacggct cgccacagca
      181 cgaccaatgt ccgggtgagc aatgaggtat agagagtgtg taagatttgc gggggacggt
      241 agggcggcaa ctccaagtgg aatgtcgatg gttccccctt caacaccgtt tgagtgagaa
      301 gtcgcgatgt aacgagtgcc atcacaacgc ccagcaaggc gacggcaata acacctccgg
      361 ccgccacaaa gccggctagg taagacggta cgaggcttcc taaaaaaacc gtggcaagga
      421 gaatctgtgt tggccatcga ccattgcaca gtgaaaagtt gttcgtcagg attgccacca
      481 ggcgttcccg tggactatca atgattcgac aggcaacgac tcctgccgca ttacagccga
      541 aacccataga cattgttaaa gcctgcttgc catgaccgcc cgcccagcgg aagagacgat
      601 ccaagttgaa tgccacccgg ggaaggtacc cgaggtcctc gagcaacgta aacaaaggga
      661 aaaagatggc cattggcggc agcatcacac tgatgaccca tgccgttgcc aggtaccccc
      721 catcaaggaa taggccaatc agccagtttg gcaaatgaac accgacggca gccgatcgca
      781 gtaagggatg gagggtttcc accaaaaacc agctcagcac ttgagacgga taattggctc
      841 cctgcacagt gatccaaatg accacgacaa atagggcaat catcgtcgga taaccccaga
      901 tggggcttgt caggatgcta tccagtttct cgtcccaagt cgaagagaca ccttccttcc
      961 ttcggacagc ccggtctgcg atccgacccg catgagcgta aatagcttca accaatgttt
     1021 gatgagcatt aggaccaatt tgccaccgcc acgctgaggc ctttgcaaga atccgttccc
     1081 gcgagtcttc ggatggtcct tcatccagtg aaggtggtcg attctcggtg gcagagtctc
     1141 ggctcactgc ttcgtgattt tgccgatgca attgggccag ttctcccgtc cgaatggcct
     1201 tgacgatgcg ctcgtcgcca tccagcagcc ttatcgcgat ccagccgagg tttgtcaaat
     1261 gggggaaagc ccgctcaagt tcttttgtca gatcctggac ggcctcgctt agttgaggtg
     1321 tactgccccc cacccgcaac gggtgacatt ggatctcccc cgtggctaca ccctcaatcg
     1381 ctttcaaaag cgtatcgatg ccgcgtccaa atcgtgctga agtggcaacg acgggaatac
     1441 ccaattctgc agcaagcagc tcggtgtcaa tgaccagtcc gtgacgctcg gcctcatcga
     1501 tcaaattgac acagagaata acgcggtgtg tgatttcaag gacttgcagg gctagattga
     1561 gattcctttc caatcttgtt gcgtccacta caacgatcgt gacgtcgggt tgtccaaaaa
     1621 gtaaaaagtt gcgagcaacc tcttcatcgt agctcgtggc aagaagggaa taggtgccag
     1681 gcagatccac aatcttgaag cgtcgagaac caaactcaaa agagccctct gccaaagcca
     1741 ccgttttacc cgcccagttg ccggtgtgct gatgcagccc tgtcaaggcg ttgaaaacag
     1801 tactcttgcc cgtgttggga tttccggcca gtgcgacgac aaagtctgcc gaccctgcct
     1861 cgacgccaag ccgccgcaac cggagcgtgg gctttgtgac acaattcgga caggtgttgc
     1921 aagaattacc acgcttcatg atatggaaat cggtacctca cgttgacgga gttggcgatg
     1981 tttcattttc ccgcttgacc aaaatggagg ccgcttggct ttttcgcaag gcgatcaagg
     2041 tacttctgac ccgaaatgcc gttgggtccc caccgaggct cgaaagttct gcccgaacct
     2101 ttgtgccggg cacgaaaccc aaatccaaga gtcgcctgcg gaccaatcca cggcacaagg
     2161 gcgcgatgcc aacaaccacg gcctcctccc ctagcctcaa atccgcaagc gtcgaaggtt
     2221 ttttctccca ctgggcaaac tcgtatctgg gaatagcctt aacattcaag ttggccgcgg
     2281 cctgaggtga caaaaccact gtttccccat gatgacgaac ctgaatttcc gtaggggcaa
     2341 gcgtttcaac ttgtagccga gcaccgaggt gtaatcctcg atcaataagc tcccggtacg
     2401 cagatgccgg ttcgtcttcc atatgggtga cttcgacgaa ctctcctggg gacattgaag
     2461 ttaatggttt cccaaggcgt ccctgagtct caccgctgtc cgtaggaatg ggatcaccgt
     2521 ggggatcata ccgagggtcc ccgagctggg caac
//
LOCUS       DAOPTR010000580         2551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_32285_length_2551_cov_3.092949, whole genome shotgun sequence.
ACCESSION   DAOPTR010000580 DAOPTR010000000
VERSION     DAOPTR010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32285_length_2551_cov_3.092949"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..2437)
                     /locus_tag="PKI05_10890"
     CDS             complement(95..2437)
                     /locus_tag="PKI05_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOA52750.1"
                     /translation="MRASQAAMLANVVRDVFYWACFSIFMTSVISQVGVSLASEPGIG
                     QIETLPGTEPLDWSGDIASRMIDAIDAFLLRQTEHTAKLRQQAWASVFSPDQWPSDWL
                     QAKRDRLAQILGVKDPRVSPVQMTILSPPGQTQPLAEGAGYDVYAVSWDVFTDLKAEG
                     ILLYPKHDQPKACVVAIPDADVSPEALIGLELGVPPASQYARRLAESGCVVVIPSLIS
                     REISKRGNVSLTHREFIYRPAYELGRHIIGYELAKILAAIDYFDSAFPTLGGNFGVIG
                     WGEGGMLALYAGALDARLGVVGVSGYFGPREHLWQAPVDRNVFGLLKDFSDAEISLLI
                     YPRSCVIEAARGPEVTIPPKLGGAPGELKTPSLEEVKNEFDRANRILQQAGKSFHLSL
                     VPSENGSGPYGTEPFLRAFLASFGTPITLSAEADPPRRIRDDPWRATRTERAIAELDR
                     HIQRLLVESPEIRKRFWAKLDTSSLERFSQSVEWYRKYFYDEIIGRFDIPLADAKPRT
                     RKVYDTLSWEGFEVVLSVFDEVFAYGILLIPKGIKPGERRPVVVCQHGLEGRPQETIE
                     GDHRAYHNFAARLADEGFVVFAPQNPYIFGDRFRTLQRKANPLGKTLFSIIVPQHQQI
                     VNWLSTLEFVDPGRIAFYGLSYGGKTAMRVPPLVPQYCAVICSADFNEWVWKNASTKS
                     RYSYVWTGEYEIFEFALGETFNYAEMATLIAPRPFMVERGHFDGVAPDETVAYEYAKV
                     RFLYNALLKIGDRTEIEWFAGPHEIHGVGTFQFLHKNLRF"
BASE COUNT          612 a          751 c          644 g          544 t
ORIGIN      
        1 cagcgattcg agccaccctc acccccgacc cctctcccac cgaacggttg aggggagatt
       61 acgggatagg ctctttgtct tcccgccagg tcttctagaa tctcaagttc ttgtgcagaa
      121 attgaaaagt tccgactccg tgaatctcat ggggtccggc gaaccattcg atttccgtcc
      181 tatcccctat cttcaagagt gcgttgtaaa gaaaacggac cttagcatac tcgtaggcaa
      241 ccgtttcgtc gggggccaca ccgtcaaagt ggccgcgctc gaccataaac ggccgcggtg
      301 cgatcagcgt agccatctcc gcataattaa acgtctcgcc caatgcgaac tcaaaaatct
      361 cgtactcgcc cgtccagacg taactatatc ggcttttcgt cgatgcgttc ttccagaccc
      421 attcgttgaa atcggccgag caaattacgg cacaatactg cggtaccaag ggcggcacgc
      481 gcatggccgt cttgcccccg taggataacc catagaatgc aatgcggcct ggatcgacaa
      541 actccaacgt gctcaaccaa ttcacaattt gctgatgttg gggcacgatg atagaaaaca
      601 atgtttttcc cagggggttg gctttccttt gcagcgtgcg aaaacgatcc ccgaagatat
      661 acgggttttg cggcgcaaaa acgacaaatc cctcatcagc gaggcgcgcg gcaaaattgt
      721 gatacgcgcg gtgatcgcct tcgatggtct cttgggggcg accctccaat ccgtgctggc
      781 aaaccacaac ggggcggcgt tcacccggtt ttattccttt tggaataagg agaatcccgt
      841 acgcaaacac ctcatcaaac acggatagaa ccacctcaaa cccttcccaa ctgagggtat
      901 catagacttt gcgagttcgc ggtttcgcat ctgccaaggg gatgtcaaac ctcccgatga
      961 tctcgtcata aaagtatttt cgataccatt ccaccgactg gctgaaccgc tcgagggaac
     1021 tcgtgtcgag tttggcccaa aagcgttttc gaatttccgg ggattcgacg agtaaccgtt
     1081 ggatatgtcg gtccaactcg gcaatggcac gctcagtgcg ggtcgctcgc cacggatcgt
     1141 cccgaattct ccgcgggggg tcggcttcgg ctgataaagt aatgggtgta ccaaatgagg
     1201 caagaaaagc acggagaaat ggttcggtac catacggccc agaaccattt tcgctcggca
     1261 ccagcgacag atggaaagac ttccctgcct gttgaaggat gcggtttgcc cggtcaaact
     1321 cgtttttgac ctcttccaac gatggggtct ttaactctcc gggtgcgcca ccaagtttgg
     1381 gcggaattgt cacctctggc cctcgagctg cctcgattac acaagatcgt gggtagatga
     1441 gaagactgat ctcggcatcc gaaaagtctt ttagcaaccc gaacacatta cgatccacag
     1501 gtgcctgcca caaatgttcg cgcggtccaa aatagccact cactcccaca accccaagac
     1561 gggcatcgag agcaccggcg tacaaggcga gcattccccc ttcgccccaa ccgatcactc
     1621 cgaagttccc acccaaggta ggaaaagcac tatcaaagta gtcaatcgca gccagaattt
     1681 tcgccagttc atagccaatg atgtgccgtc ccagctcata agctgggcga taaatgaact
     1741 cacggtgcgt caatgacaca ttgccccgtt tgctgatttc cctcgaaatc aagctcggaa
     1801 tcacaaccac acatccgctt tccgcaagcc gccgagcata ttgcgaagca ggtgggacac
     1861 cgagttccag gccgatcaag gcctcgggcg atacatcggc atcgggaatc gccacgacac
     1921 acgccttggg ttggtcatgt ttgggataga gaaggattcc ttcggccttc aggtccgtaa
     1981 acacgtccca actcacggca taaacatcgt agcccgcccc ttccgccaag ggctgggtct
     2041 gtcctggtgg gctaaggatc gtcatctgca caggcgagac ccgcgggtcc ttcacgccaa
     2101 gaatttgagc aaggcgatcc cgtttcgcct ggagccagtc gctcggccat tgatcggggg
     2161 aaaatacgga tgcccaggcc tgttgtcgca atttagcggt gtgctcagtc tggcgaagga
     2221 ggaatgcgtc gatcgcgtcg atcatccgcg aggctatgtc gcccgaccag tcgagaggct
     2281 cagtgcccgg caacgtctca atctggccga ttcccggctc ggaggccaaa gaaacaccga
     2341 cttggctgat aacactggtc atgaagatgg agaaacatgc ccaataaaac acgtccctaa
     2401 cgacgttcgc cagcatcgct gcctgactag ctctcatgat tgaacctccg ctcagtatag
     2461 tttccgtcca accaggacgt cggcttgcca tgaccgacac accatccgat ttctgccgct
     2521 aaccagtgta cgagaacccg taaggctcaa c
//
LOCUS       DAOPTR010000581         2551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_32289_length_2551_cov_2.612981, whole genome shotgun sequence.
ACCESSION   DAOPTR010000581 DAOPTR010000000
VERSION     DAOPTR010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32289_length_2551_cov_2.612981"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1067)
                     /locus_tag="PKI05_10895"
     CDS             complement(93..1067)
                     /locus_tag="PKI05_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52751.1"
                     /translation="MMARFRSSVLGNDAVQATGSPAGLVVAIVALVLFGGCGTTKWTE
                     TKRTATEQLLISDSMDQAVSRIDFRALAGKTVFLDDTFLKDVVDAQYLVSSLRQQLLA
                     HGAILKQKREEADYVVEVRSGAVGTDMHSLLVGVPQINLPSVAAAAGLPSSFPEIPLI
                     KRTKQTAVTKILLFAYNRETGRPLWQSGTVLAKSDAQDIWIFFAGPFQRGSIYEGTQL
                     AGDQLPISIPLIDPKEPRTAARLVEEAYYIEEPDPKSTRSRLTSKTETASPPDQRTDH
                     ATNLSHGQTAPASETTPPPSWSTTQMAFPGPVFPPDGPALGSPYAPQR"
     gene            1585..>2551
                     /locus_tag="PKI05_10900"
     CDS             1585..>2551
                     /locus_tag="PKI05_10900"
                     /inference="COORDINATES: protein motif:HMM:NF039401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6288 domain-containing protein"
                     /protein_id="HOA52752.1"
                     /translation="MFVVTFTRPIVVLVMLSLATAALSADIPDFTRGDRIPEGAVHDW
                     NLGPTGARGWMYCDKLVTSDARQILITKVEKGSPADGVLAVGDVILGVGGKRFSYDPR
                     TEFGKAITLAESEAGGGRLSLIRWRAGETKEVVIKLPVLGTYSPTAPYNCPKSRIILE
                     RGCAALARRIADPNYRPDPIPRSLNALALLASGNPEYLELVKMEAHRAARFKTDGFQS
                     WYYGYLMILLAEYKLATGDDSVMPGLTRLALETANGQSKVGSWGHKFARPDGGLDGYG
                     MMNSPGIPLTIGLILARKAGVDDPKVSEAIEKSARLLRFYVGKGAV"
BASE COUNT          546 a          664 c          771 g          570 t
ORIGIN      
        1 ttctttttgg ggtgtttctt ggttgagcgg gcaacccctg tcaaggattt gattttatgt
       61 ctctttgcaa tgattgcccc gaccctgggc aatcatcgct gcggggcata gggcgatcca
      121 agggcagggc catcaggcgg aaagacggga ccgggaaagg ccatttgggt cgtactccac
      181 gacggcggcg gagtcgtttc ggacgcaggt gccgtctgtc cgtgggaaag gtttgtggcg
      241 tgatccgttc gctggtccgg cggggacgcc gtttctgttt tgctggtgag ccttgatcgc
      301 gttgacttag ggtcaggttc ttcgatgtaa taggcctctt ccacaaggcg ggcggccgtc
      361 cgcggctctt tcggatcgat caggggaata ctaatcggta actggtcccc ggcgagttgt
      421 gttccctcgt agatggaacc cctttggaac ggcccagcga agaaaatcca aatatcctgg
      481 gcatcgctct tggcgaggac agtgccggat tgccacaacg gtcgcccggt ttctcgattg
      541 taggcaaata gcagaatttt cgtgacagcc gtttgcttgg tccgtttgat aaggggaatc
      601 tctgggaagc tcgaaggaag tcccgcggcg gccgccactg agggcaaatt gatttgcgga
      661 acccctacca agaggctgtg catgtccgtt cccacggcgc ccgatcggac ctcgaccacg
      721 tagtcggcct cctctcgctt ttgcttgagg atggccccgt gcgccaacag ttgttgccgt
      781 agcgaactga ccaagtactg agcatcaact acgtccttaa ggaacgtgtc gtccaaaaaa
      841 actgtcttac cggccaaggc gcggaaatcg atccgcgaga cggcctgatc catggaatcg
      901 gagatcaaaa gttgctccgt cgccgtccgc ttggtctccg tccatttggt cgtgccacac
      961 ccaccaaaga ggaccagcgc tacgatggcc acaaccaaac cggcaggcga gcccgtggcc
     1021 tgaacagcat cattaccaag aacactgctg cgaaaacgtg ccatcatggg ccgtccatgc
     1081 catccttcgg atcacgatta acgtgtcgga atatcccgtt gcctccgttt tttgcctaat
     1141 tacagccggg gaagtatagc caaacggttt cgtcgggaaa agacgatttg ccgcgggtga
     1201 taaatcgggg gaaagacgta ggagggggtg aatcgctcgt ggggtggtcc cctcaggttg
     1261 gcgggcttgt cggcgtgccg taggaggatt ggatagatgt accatttcct ctcgcccccg
     1321 agcgaagggg acgatcgtat gatcgatccg acgtacattg aatgccttgc aaaaaagttt
     1381 caagtcacac ggtcatctgc caggatcgat tgcgattttg tgttaatcta tggagaacat
     1441 caaagattcg gcgcactctg caaaaagcgg cgtatccaga attggggtgg caacctgcct
     1501 ctgttgtcca agcggcgcta tccacggtgt taggggcgat tcgttggcca agatcctttc
     1561 accaaagaac aacggagcta ggcaatgttt gtggtaacgt ttacaaggcc gatcgtcgta
     1621 ttggtcatgc tgtccttggc aacggcggcc ctatccgcag atatacccga tttcacgcga
     1681 ggtgaccgaa ttccggaagg ggccgtacac gattggaatc ttggcccaac tggtgcccgg
     1741 gggtggatgt attgtgacaa actagtgacc agcgacgctc ggcagattct cattaccaag
     1801 gttgagaagg gttctccggc tgatggcgtc ttggcggtgg gagacgtgat ccttggagtg
     1861 ggcggcaagc gattttcgta tgatccccgc acggaattcg gaaaggcgat cacgctggca
     1921 gaatctgaag cagggggcgg tcggttatca ctgatccgct ggcgtgccgg ggaaacgaag
     1981 gaggtggtga tcaagctgcc agtgctcgga acatacagcc ccacagcgcc gtacaactgt
     2041 cccaagtctc gaatcatcct cgaacgaggt tgcgcggcgc ttgctcgccg gatcgccgat
     2101 cccaattatc gcccggaccc cattccgcga agtctcaacg cgttggccct tttggcaagt
     2161 gggaatcctg aatatcttga gttggtcaaa atggaggctc acagggcggc caggtttaaa
     2221 acagatggtt tccaaagttg gtattacggc tacctgatga tcctgctcgc tgaatacaag
     2281 ctggcgacgg gggacgattc ggtgatgccg gggctcaccc gtcttgccct ggaaacagcg
     2341 aacggacaaa gcaaggtggg ttcatgggga cacaaatttg ccaggccgga tggcggactc
     2401 gacggttacg ggatgatgaa ttcgcccggt attcccctta cgattggcct tatcttggcc
     2461 cgcaaagctg gcgtggatga tcccaaagtc agcgaagcaa tcgagaaaag cgctcggcta
     2521 cttcggttct acgtgggaaa aggtgccgtc c
//
LOCUS       DAOPTR010000582         2548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_32336_length_2548_cov_3.198155, whole genome shotgun sequence.
ACCESSION   DAOPTR010000582 DAOPTR010000000
VERSION     DAOPTR010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32336_length_2548_cov_3.198155"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PKI05_10905"
     CDS             complement(<1..189)
                     /locus_tag="PKI05_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52753.1"
                     /translation="MINVFQKAQGQDARPTVASLFYRVPCFDHTRAHREKLEGLKKTV
                     SAPLQKYPFAGPMIATLCP"
     gene            206..2194
                     /locus_tag="PKI05_10910"
     CDS             206..2194
                     /locus_tag="PKI05_10910"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOA52754.1"
                     /translation="MSEKGVISAEGQTRALSAAVWERTLREVLAAACEASDAISYLRF
                     QTPHLRRVLEAQFFGFLVFAGNGRSWEWVTTIGETKYATECDWGRLADRHGPLTEGPW
                     CLQPLAEGPMGRIWIAARFDHPQTAAQVRELLVELAETLNTALHFVSERSRISEQLKR
                     QTTLLRIAQRWYQARQLRPLLSEIAQAAAEFLRADRATIFLWNRDAKMLIGRPALGVD
                     DELRIPDDAGIAGKVLRTGQPVRVSSTSPDRDEIDRRVDKQLGYRTETLICVPLRTRG
                     GRVLGVFEVLNKKDGNFTADDETTLLELAEQAATALESVRDYEEALLTRRQIVDEAAR
                     RVQLIGQSPAIVRLRRDIERLAQADLPVLILGENGTGKEVVAQMLHYLGPRRESPFVA
                     VNCAAIPESLAESELFGHEKGAFTDAHQTRPGKFELAQNGTVFLDEVAELSLAAQAKL
                     LRVLEERKIIRVGGTEAIPVYARIITATNQNLQQRVAEGRFREDLFYRLNTVILTLPP
                     LRERGDDIILLAEHFLAEFATRIRRPVPELTPEAKAFLMQYPWPGNVRELRNVMERVV
                     YLCSKTSIEPSDLGLHPEAAGVKRIGAFGPGLKLREATRRFQTEYILRTIEVSQNNVT
                     LAARQLGLHRANLYRKMRQLGIQLRRENEDSEPQPEKT"
     gene            complement(2255..>2548)
                     /locus_tag="PKI05_10915"
     CDS             complement(2255..>2548)
                     /locus_tag="PKI05_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52755.1"
                     /translation="VNLCDEPSFDRRNVILETAVQLLGRDIVVAHAKDVLTHARPRTP
                     GGSPTLDYVHYFRQLKGIGFAGPLLIHSVPEDKVPATIKFLREMAQQVGIHLE"
BASE COUNT          574 a          677 c          745 g          552 t
ORIGIN      
        1 agggcaaagt gtggctatca ttggtcccgc aaatgggtat ttttgtaacg gggcgctgac
       61 tgttttcttc aaaccctcca gcttctcccg gtgcgctctg gtatgatcaa agcagggaac
      121 acgataaaat aggcttgcta ctgtcggacg agcatcttgt ccttgagctt tttgaaagac
      181 attgatcata tcgaggagta cggttgtgag tgaaaaagga gtcatttctg ccgagggcca
      241 gacacgcgcg ctatctgccg ctgtatggga acgaacactg cgggaagtcc ttgcggctgc
      301 ctgtgaggcg tccgacgcca tcagctattt gcggtttcag accccgcatt tgcgacgcgt
      361 cctcgaagcc cagttctttg ggtttctcgt cttcgcaggc aacggacgtt cctgggaatg
      421 ggtgaccacc atcggcgaga caaaatatgc aacggagtgt gattggggaa ggctagccga
      481 tcgccacggc ccgctgacgg aaggcccctg gtgtctgcaa ccgttggcag aggggcctat
      541 gggccgcatt tggattgcgg cccgttttga tcatccccag acggcggcac aggtccgaga
      601 actcctcgtg gagttggcag agaccctaaa tacggccctt cactttgtga gtgaacgatc
      661 ccggatttca gaacaactca agcgtcaaac cacccttttg agaattgcac agcgttggta
      721 tcaagcgcgg cagcttcggc cccttttgag cgagattgcc caggccgcgg ccgagttctt
      781 gcgggcagat cgggcgacga tctttctttg gaaccgggat gccaagatgc tgattggccg
      841 ccctgcccta ggcgtagatg atgaactccg aattccggat gatgccggca ttgcggggaa
      901 ggtcttacga accggtcagc cggtccgtgt ctcaagcacc tcgcccgacc gtgatgagat
      961 cgaccgccga gtggacaagc agctaggcta ccgcaccgag acacttattt gtgttccgct
     1021 gcgtacccgc ggggggcgtg ttcttggggt cttcgaagtc ctcaataaga aagacgggaa
     1081 tttcacggca gacgacgaga ctaccttgct tgaacttgcc gaacaagcgg ccacggcctt
     1141 ggagagcgtg cgagattatg aagaagcctt attgacaagg cgacaaatcg tagatgaagc
     1201 ggcacggcgg gtgcaactga ttggacaatc gcctgcgatt gtccgtctcc gccgggatat
     1261 cgagcgattg gctcaggccg atttgcccgt actgatcttg ggcgaaaacg gtacaggcaa
     1321 ggaggtcgtg gcccagatgc tccattacct tggcccacgt cgtgaaagtc ccttcgtggc
     1381 cgtcaattgc gcagctatcc ccgaatcgct agcggaaagt gaattgtttg gccacgaaaa
     1441 gggtgccttc acggatgccc atcaaacgcg accgggcaag tttgaattgg cccaaaacgg
     1501 gacggtcttt ctcgatgagg ttgccgagtt aagtctggcg gcccaggcca aactcctgcg
     1561 ggtcctcgag gaacgaaaaa tcattcgtgt tgggggaacc gaggccattc cggtttatgc
     1621 ccgaatcatt acggctacga atcaaaacct tcaacagcgg gttgcggaag ggcgctttcg
     1681 cgaggatctt ttctatcgcc tgaatacggt catcctaact ttgccgccac ttcgggaacg
     1741 aggggatgac attatcctct tggccgagca tttcctggcc gagtttgcca caaggattcg
     1801 acgtcctgtg ccggaactca cacccgaggc caaggcattt ctcatgcagt acccctggcc
     1861 gggcaatgtg cgagaactgc ggaatgtcat ggagcgcgtc gtttacttgt gctcgaagac
     1921 gtcgattgaa ccgtccgatc ttggcttgca cccggaggca gcgggcgtga aacggattgg
     1981 ggcctttggc ccgggactta agctccgtga agcgactcgg cgattccaaa ccgagtatat
     2041 cctgcgaacg atcgaagtct cgcagaacaa cgtaaccttg gcagcccgac aattgggtct
     2101 gcaccgagct aatttgtacc gcaaaatgcg gcaattgggc atccagttgc ggcgggagaa
     2161 tgaggactcc gaaccacaac ccgaaaagac gtgattgaat ggcaacccgt atcggcgcag
     2221 ggcgggcatg gccacgaagg cgactaagcg ggtcttactc aagatggatt cccacttgct
     2281 gagccatctc ccgaagaaac tttattgtgg ctggaacttt gtcctctgga acactgtgga
     2341 tgagtaaagg tccagcaaac ccaatcccct ttaattgacg gaaatagtgg acgtagtcga
     2401 gcgtggggga gccacctgga gttcttggac gcgcatgagt taagacatct ttggcatgcg
     2461 ccacgacgat atcccgaccg agtaactgca ccgcggtttc cagaataacg ttgcggcgat
     2521 caaaagacgg ctcatcacaa agattcac
//
LOCUS       DAOPTR010000583         2546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_32360_length_2546_cov_3.468888, whole genome shotgun sequence.
ACCESSION   DAOPTR010000583 DAOPTR010000000
VERSION     DAOPTR010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32360_length_2546_cov_3.468888"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PKI05_10920"
     CDS             complement(<1..634)
                     /locus_tag="PKI05_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52756.1"
                     /translation="MIKNETTSPPADSVKHAVSPPPTTDVLRLTENVKLLLEHGVQVA
                     KATKAAAIALLWPGKAEWTRVRNLVQDHKLIVVVDTQAQAKAPQEAGLLTVALNLSAA
                     SPMERLSQTLVECVSKDLVSVTDDLVVIYGAFDAGTLDSLGVFKLSDYLGRLTIRELR
                     QLQTDIPLETLKTVINLAIEIGREGREGKPVGTMFVVGDTRKVLAHTHPLG"
     gene            1089..1610
                     /locus_tag="PKI05_10925"
     CDS             1089..1610
                     /locus_tag="PKI05_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOA52757.1"
                     /translation="MKCQKCDRPATYHITELTGPTPVEVHLCEQHAQEYLGRGSEPSV
                     EMTQVAEQMAQQLAKHMVVGQAAEEMKRLDQQACPYCGITFYKFRNQGRLGCPHDYEV
                     FREQLEVLLANIHGDTPHKGKVSHHGPQASKIRTELIRLRREMREAVENEEYERASQL
                     RDEIRRIESGEVS"
     gene            1739..>2546
                     /locus_tag="PKI05_10930"
     CDS             1739..>2546
                     /locus_tag="PKI05_10930"
                     /EC_number="2.7.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--guanido phosphotransferase"
                     /protein_id="HOA52758.1"
                     /translation="MLTVDDLMQGCGEWLQGKGRDNDIVISTRIRLARNLADFPFTSI
                     ASEAEQRQILDRVRDVIAASPFSEQLLYVPMEELSELDRQFLVERHLISRELAEGQGP
                     KAVFIDHAERFSIMVNEEDHLRIQAMQSGFEPEPTWALINELDDYLGQKLVFAYHQEW
                     GFLTACPTNVGTGMRVSVMLHLPASVIVKQIEQIFRSLEKLELVVRGLYGEGSQALGD
                     FYQVSNQVTLGRSEGELIRKMLDVIPAIVGAERKARQFLLDEGSQRVHDRI"
BASE COUNT          608 a          618 c          750 g          570 t
ORIGIN      
        1 aacctaacgg atgagtgtga gccaaaacct tccgagtatc acccaccacg aacatagtgc
       61 caacgggttt gccctcgcgg ccttctcgtc cgatttcgat ggcgagattg atcacagtct
      121 ttagcgtttc taagggaatg tccgtctgga gttggcgaag ttcccgaatc gtcagtcgcc
      181 ccaggtaatc tgagagttta aacactccca aagaatcgag cgttcctgcg tcaaaagcgc
      241 cataaatcac caccaagtcg tctgtcactg acaccaaatc tttactgaca cactcaacaa
      301 gtgtctggga aagccgttcc attggggagg ccgcagaaag attgagggcg acggtcaaga
      361 gtccggcctc ctgcggcgct tttgcctggg cttgggtgtc aacgaccacg atgagtttgt
      421 gatcttgcac cagattgcgg actcgagtcc attcggcttt gcccggccag agaagcgcga
      481 tcgctgctgc cttggtggct ttggctacct gaactccatg ttctaggaga agtttgacgt
      541 tctcggtgag tcgcaggacg tcggtagtgg gaggggggga aacggcatgt tttacgctgt
      601 cggccggcgg ggaggttgtc tcatttttga tcatagacag tcgaattatt agcaagaaga
      661 aatttagcag gacgacgggc gatcgcactc ccaagctggg cttgatactc gcgaagtaaa
      721 cgacactgcc gtgtcaagac atccctggac acagccttac cgggcacgcg acgatcaatt
      781 aacaccgcaa aatcatcata cccctttctg tccacgtgga caacggcgtt ttacactggg
      841 ttgaagaggg cttatcgaag taggaacgac attggtcgta gatcggtaag gaggataagg
      901 ccggattttg atcgatgttg acacaagctt gaggcgcgtt agaatccaag tgaattgcct
      961 gttttcccaa cggaaaaagg gttgcgtctc ggggaagtgg ggggcgctgt acattgatgg
     1021 cggttgatgc gaccattgtt cagcggtgtc tgaaaggacg tcgctgtctt tattaaaagg
     1081 gtcatattat gaaatgtcaa aaatgcgatc gccctgcgac gtaccatatt accgaattga
     1141 ccggtcccac gccggtcgag gtccatctgt gcgagcagca cgcccaggag tacttgggtc
     1201 ggggtagtga gccatccgtc gagatgacgc aagtggccga gcagatggct cagcagttgg
     1261 ccaagcacat ggtggttggt caagccgccg aggaaatgaa acggcttgac cagcaggcat
     1321 gcccctactg tggaatcact ttttataaat tccggaatca gggacggttg gggtgtccgc
     1381 acgattacga ggtgttccgc gaacaactgg aagtcctttt ggcgaacatt cacggagata
     1441 ctccccataa gggaaaagta tctcatcacg ggccgcaagc ctcgaaaatc cgcaccgagt
     1501 tgattcggct tcggcgggag atgcgggaag cggtggaaaa tgaggaatac gaacgggcat
     1561 ctcaactgcg tgacgagatt cggcgaatcg aatcaggaga ggtctcctaa agagcgtcga
     1621 tgttgccgag gataggacca tcgttttcct cgccaaggca acttgactga gcgaccttcc
     1681 ggaaaagccc aaagcctttt acagagacca gggactcacc agagatacga gtagcacaat
     1741 gttgacggtt gatgatttga tgcagggttg tggggaatgg ctacagggta agggtcggga
     1801 caatgacatc gttatttcca cccgaatccg cctggcccgt aatctggcgg actttccttt
     1861 caccagtatc gccagtgaag cagagcaacg gcagatcctt gatcgtgtga gagacgtcat
     1921 tgcggccagt ccattctctg agcaacttct ctatgttccc atggaggagc tttccgagct
     1981 agaccgccag tttcttgtgg aacgccatct catcagccgc gaattggcag aagggcaggg
     2041 gcccaaggcc gtttttatcg accatgccga gcgtttcagt atcatggtga acgaagagga
     2101 tcatcttcga attcaagcga tgcagagcgg ttttgaaccg gagccaactt gggccctgat
     2161 taacgagttg gacgattacc tcggacagaa gctggtcttt gcgtaccatc aagagtgggg
     2221 gtttctcacg gcctgcccga caaatgtggg aacggggatg cgggtaagtg tcatgctgca
     2281 tttgcccgcc tcggtcatcg ttaagcagat tgaacagatt ttccgcagcc tagagaaact
     2341 ggagttggtc gtgcggggat tgtacgggga aggctcgcag gctctaggcg atttctacca
     2401 ggttagcaac caggtgacgc tgggccgtag cgagggggag ttgattcgca agatgctgga
     2461 tgtcatcccg gcgattgttg gcgccgaaag gaaggcccga cagttcctcc ttgacgaagg
     2521 aagccagcgg gttcacgacc gaatca
//
LOCUS       DAOPTR010000584         2539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_32471_length_2539_cov_2.886876, whole genome shotgun sequence.
ACCESSION   DAOPTR010000584 DAOPTR010000000
VERSION     DAOPTR010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32471_length_2539_cov_2.886876"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(276..983)
                     /locus_tag="PKI05_10935"
     CDS             complement(276..983)
                     /locus_tag="PKI05_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA52759.1"
                     /translation="MPSLILEQITKTYVTSAEPVEVLRGIDLSVPSSDSLAIVGPSGS
                     GKSTLLNIIGGLDRPTSGRVIVDGEDITRMSELSLSKFRNQKIGFVFQDHHLLPQCSV
                     LENVLIPSLAQGRPSKEHLQWAEYLLERVGLSHRAHHRPSELSGGERQRAALARALIL
                     RPVLILADEPTGNLDSVAGRKVAELLLELQAENQAILITVTHSPFVASMMSRRLHLED
                     GRLVERPQNVSLTSNQL"
     gene            complement(1079..2059)
                     /locus_tag="PKI05_10940"
     CDS             complement(1079..2059)
                     /locus_tag="PKI05_10940"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOA52760.1"
                     /translation="MTDPHSVTRRGFLGSLSAGGIMAGPTLLHFAQVTDSEPQVASSY
                     RLIDTHVYLFQWPFRLIHSAETAELVRSLKNAGVTCAWAGSLESLLHRDIASVNARLH
                     EECRKYGDGFLVPCGTINPMLPDWTEDFRQCVENYKMRVLRLFPGYHGYTLDIPEVGR
                     LLALAHKCEIGIQITIEMEDVRAQNPLLPVKPVEVTPLGRWLEDFPALRLMLLNCHRA
                     VPPQQLAKLVTIGWVYVDLGMLEGIAGLERLTSVIPIERICFGSFAPVFYFESALLKL
                     AESHLPGGSLDAICYQNAETFSHRQPPYPRKGGFPMPYQRTCAVWLASSG"
     gene            complement(2056..>2539)
                     /locus_tag="PKI05_10945"
     CDS             complement(2056..>2539)
                     /locus_tag="PKI05_10945"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOA52761.1"
                     /translation="KLWVARRCCDSALDPIVALAHDCDAVVLQHTWIKTTGNLPGEST
                     PHDLACLARRHPDAVFIAAHSGGGNWEIGLRAFCETPNVYAELSGSDPTSGFTEMAMR
                     LLGPRRVIFGSDAPGRSFATQLGKVLGASVDDDTKRLILADNLKKLLAPILKKKGVPV
                     "
BASE COUNT          613 a          672 c          693 g          561 t
ORIGIN      
        1 gagaatgcac tttcgacacg atacgtgtcc actgtgtggc cgatagttta acgagattat
       61 ggtcagaata caacgaaccc agattgtcca agttgccctc tcgcttcatc tcgcttggca
      121 agcaaggtgt acggcgagtg gtaaccagag atctagtcgg ccggtgtact catccgctga
      181 cagacttgct ctcgcaaatc agggcatagc atcgcgtgct ggctaactat atcagaacgc
      241 tgatgttgat gtgcgctgga aaaaagtgac ttatctcata actggttgga tgtaagcgaa
      301 acgttttgcg gtctctcgac gaggcggccg tcttcaagat gtaagcggcg cgacatcatg
      361 gaagcaacga atggactatg agtaacggtg atcaatatgg cctggttttc ggcctgaagt
      421 tccagcaaca gttcggccac cttgcgtccc gcaacagaat ctaggttccc tgtcggttca
      481 tcggcgagga taaggacagg gcgaaggatc aatgcccttg ccaaggcggc ccgctgccgt
      541 tccccgcccg agagttctga tggtcgatgg tgggctcgat gcgagaggcc gactcgctcc
      601 aaaaggtatt ctgcccattg gaggtgttcc ttgcttggtc tgccttgtgc cagcgagggg
      661 atcaggacat tttctaagac ggagcactga ggaaggaggt gatgatcttg aaacacaaat
      721 ccaattttct gattcctgaa ctttgacaac gaaagttctg acatccgcgt gatgtcttct
      781 ccatccacaa tgacgcgccc actcgtcggc cgatccaacc cgccgatgat attgaggagg
      841 gtgctcttgc ccgagccgct cggtcccaca atggccaagc tgtcacttga tggcactgaa
      901 aggtcgatgc cccggaggac ctcgaccggt tccgctgatg tcacgtaagt cttggtaatt
      961 tgttccaaaa taagagatgg catgaaacac ctcagcagtg atgatgattt tgccgaacca
     1021 cacaagggtc caccatcaag tataacggac tcggcgcgcg ggcagaggct gagattggct
     1081 atccggaact ggcaagccaa accgcgcaag tcctttggta aggcatcgga aagccgcctt
     1141 tacgtggata cggcggttgc cggtgtgaga atgtttccgc attttgataa cagatggcgt
     1201 ccaaactccc cccgggcaaa tgggactcgg ccagtttgag caaggccgac tcaaaataga
     1261 acactggggc aaaagaacca aagcatattc gctcgatggg gattacactt gttagccgtt
     1321 ccaatccggc gattccttcc agcatgccta gatcgacata aacccatcct attgtaacga
     1381 gtttagccag ttgctgtggt ggaactgcac ggtggcaatt gagcaacatc agccggaggg
     1441 cgggaaaatc ctccagccaa cggcccaagg gcgttacctc aacgggcttc accggcaaga
     1501 gtggattctg ggcacgaaca tcctccatct cgatggtgat ttggatgccg atttcacact
     1561 tgtgcgctaa ggctaggagt cgtcccacct ccggtatgtc gagggtataa ccgtgatatc
     1621 ctggaaagag cctgagaacg cgcatcttgt agttttcaac acactggcga aaatcctcgg
     1681 tccagtccgg caacatggga ttgatcgtcc cacatggcac cagaaagcca tcgccatatt
     1741 tgcggcattc ctcgtgaagt cgcgcattga ccgatgcaat atccctatgc agtaacgatt
     1801 cgagacttcc cgcccatgcg caggtaaccc ctgcgttctt gagggaccta accaattccg
     1861 cggtctctgc actgtgtatt agacgaaacg gccattggaa aagataaaca tgggtatcga
     1921 tcagccgata ggaagaagca acttgtggtt cactgtccgt gacctgagca aaatgcagta
     1981 acgtcggccc cgccataatg ccgcccgcgg acaacgaacc gagaaaacca cgacgggtta
     2041 ccgagtgcgg atcggtcata cagggactcc ctttttcttc agaatgggag ccaggagttt
     2101 cttgagatta tctgccagga tgagtcgttt ggtgtcgtca tcaactgagg ctcccaagac
     2161 cttccccaac tgcgttgcga agcttcgccc aggggcgtcg cttccgaaga tgactcgcct
     2221 gggaccaaga agtcgcattg ccatttcagt aaaacccgag gtcggatcac taccacttag
     2281 ttcggcgtag acgttgggtg tttcgcaaaa ggctcgtagc ccgatctccc aattaccccc
     2341 gccactgtgg gctgcaataa agacagcgtc ggggtggcgt cgggctaagc acgccagatc
     2401 atgaggggtg gactctcccg ggagattgcc tgtggtctta atccatgtgt gctgtaggac
     2461 aacagcgtca cagtcatggg ccaaagctac aataggatca agggccgaat cgcaacagcg
     2521 gcgcgctacc cagagcttg
//
LOCUS       DAOPTR010000585         2536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_32511_length_2536_cov_3.542926, whole genome shotgun sequence.
ACCESSION   DAOPTR010000585 DAOPTR010000000
VERSION     DAOPTR010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32511_length_2536_cov_3.542926"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..1511
                     /locus_tag="PKI05_10950"
     CDS             360..1511
                     /locus_tag="PKI05_10950"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HOA52762.1"
                     /translation="MIVGVPREIYPEERRVAIVPATVPSLTKAGHVVRVETGAGMAAG
                     FSDEEYRSKGAEVVSRRDVFASEVLLGVRWDIPGEARAETLDLVRPKQVIVGSWDPLS
                     KRENLVAWAEKGVSIFALELLPRITRAQSMDILSSMATLAGYRAVILAAMALPKIFPM
                     LMTAAGTLTPAKVFVVGAGVAGLQAIATARRLGAAVSAYDVRPAVKEQVQSLGAKFIE
                     LDLETSGAEDQRGYAKAMDESFYQKQREMMFRVVAESDVVITTASVPGKKAPVLITRE
                     MVAGMARGSVIVDLAAERGGNCELTQPGKTVIENGVTILGPTNLPSEIPFHASQMFSR
                     NISTFFLHLCKTGSIPFETDDEIIRDTLVVRDGEIVHPMLVGSSAGATA"
     gene            1549..1896
                     /locus_tag="PKI05_10955"
     CDS             1549..1896
                     /locus_tag="PKI05_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOA52763.1"
                     /translation="MLNNCFLLAEQINEVAEVTQGSQLPLALALTIFVLAIFVGFEII
                     TKVPPTLHTPLMSGSNAISGITIVGAIIAAGAGHSGLATLLGTIAVVLATINVVGGFL
                     VTNRMLHMFKRKS"
     gene            1974..>2536
                     /locus_tag="PKI05_10960"
     CDS             1974..>2536
                     /locus_tag="PKI05_10960"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOA52764.1"
                     /translation="MDRGTVITLAYLLASVLFILGIKGLTHPRTAVRGNLLGALGMLI
                     AIVATLIHWSIIEYHWIILGLVVGAVIGAVLALKVKMTAMPQMVALLNGFGGGASVLV
                     AGAELTRTPPAPYSDALIATYASAVIGAVTFWGSLLAFAKLEEYRAFRQPIRYPLLQP
                     INAVLGLLALALCVIGIGQPQYIGLYWG"
BASE COUNT          544 a          630 c          743 g          619 t
ORIGIN      
        1 ttacccactt ttgacactgt aacatgccgg gaaacatgtg gtgatgcggg aatgcttcgg
       61 tcgcgcggca ctgaaaaaga atcgggaatt tgtgagcatt tcggacgatc gacgacgtga
      121 aaatgggacg taagagacgc aattcacagc aaaacgacaa aataggcaag atgcgcattt
      181 tgccgcacaa gccgtacttt ttgtcgccac cgtgcccgca agtttcaccc gaaacgtacc
      241 gtggctgtaa gaaaggcaag gaaaccccgg gactccgtac cttaactttc tgcgacatct
      301 cacctaaaat tggttttttc cttgtaatcc gttggttttc gcaaggagtt acgatccaga
      361 tgattgtcgg tgtaccgcgc gaaatttatc cggaagaacg tcgagtcgcg atcgtgccgg
      421 ctactgtccc gtcattaacg aaggcgggcc atgttgtgcg cgtggaaacg ggagctggga
      481 tggccgccgg gttctcggac gaggagtacc gctccaaggg cgcagaggtc gtttcgcggc
      541 gtgatgtttt tgcttctgag gtacttttag gagtccggtg ggatatccca ggcgaggcgc
      601 gagcggaaac actcgatttg gtgcgtccaa aacaggtgat cgtggggagt tgggaccctc
      661 tttccaaacg agagaacttg gtagcctggg cagagaaggg ggtttccatc ttcgccctgg
      721 agctactccc ccgcatcacc cgggcacaaa gcatggatat tctttcatcc atggcgaccc
      781 tggcaggata tcgtgcggtc atcttggctg cgatggcact tcccaaaata ttcccgatgc
      841 tgatgacggc cgccgggacg ttgaccccag caaaagtatt tgttgtgggt gccggcgtgg
      901 ccggtctcca ggctattgcc acagcaaggc ggctgggggc agccgtttcc gcttatgacg
      961 tccgcccggc agttaaggaa caagtgcaga gcttgggcgc caagtttatc gagttagatt
     1021 tggaaacgag cggggccgag gaccagcgcg gttacgcgaa agccatggac gagtccttct
     1081 atcaaaaaca aagagagatg atgtttcgag tggtagcgga aagtgatgtc gtaattacaa
     1141 cggcatctgt gcccggtaaa aaggcacccg tcctgatcac gcgggagatg gtggccggca
     1201 tggccagggg ttccgtcatc gttgatttgg cagcggagcg gggcggcaac tgtgaattga
     1261 cgcagccggg gaaaactgtg atcgaaaatg gagtcacgat tcttgggcca acgaatttgc
     1321 cgtccgagat tcccttccat gccagccaga tgttttcccg caatatctca acattcttcc
     1381 tgcacttgtg caaaaccggc agcatcccat ttgagacgga tgatgagatc attcgcgaca
     1441 cacttgtggt acgggatgga gagattgttc acccgatgtt agtgggcagc tccgccgggg
     1501 caaccgcgtg attggtcatc tcgaaagacg acacgggaga gttcgcttat gcttaacaat
     1561 tgttttttgc tcgctgaaca gatcaatgaa gtggccgagg tcactcaagg ctcacaactg
     1621 cccttggcct tggcattgac gattttcgtc ttggcaatct ttgtgggctt tgaaatcatt
     1681 accaaagtcc cccccacgct tcacacgccc ttaatgtccg gctccaatgc gatctccggg
     1741 atcacgattg tcggggctat catcgcggct ggggccggac actctggtct ggcaaccctt
     1801 ctggggacga ttgccgtcgt ccttgctacg atcaacgtgg tcgggggttt tctggtgacg
     1861 aaccgaatgc tccatatgtt taagcgaaag tcctagccgg gcagcaagtt ggtcgttctt
     1921 aacatcggcg cgattttgca aagacgtggt catttcgtaa aggtgaggta gctgtggatc
     1981 gcgggactgt cataaccctg gcatatcttc tggcttctgt cctcttcatt ttggggatta
     2041 aaggtcttac acatccgaga acggcagtgc ggggcaattt gctgggcgcc ctgggcatgc
     2101 taattgcgat tgtggccacg cttatccatt ggtctatcat cgaataccat tggatcattc
     2161 ttggattggt tgtgggggcg gtgataggtg ccgttctggc gttaaaagta aagatgaccg
     2221 ccatgccaca aatggtggca ctccttaatg ggtttggggg tggggcgtcg gtgctggtgg
     2281 caggggcaga attaacccgt acaccgccgg caccttatag cgatgccctg attgcaacct
     2341 acgcttctgc cgtcatcggc gccgtaactt tttggggaag ccttttggcg ttcgccaaac
     2401 tcgaggaata tagggccttt cgccagccga ttcgttatcc ccttttgcag ccaatcaatg
     2461 cggtcctagg acttttggcg ttggctttgt gtgtgattgg catcggccaa ccccagtaca
     2521 tcggtcttta ttgggg
//
LOCUS       DAOPTR010000586         2536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_32514_length_2536_cov_3.062072, whole genome shotgun sequence.
ACCESSION   DAOPTR010000586 DAOPTR010000000
VERSION     DAOPTR010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32514_length_2536_cov_3.062072"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PKI05_10965"
     CDS             complement(<1..247)
                     /locus_tag="PKI05_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52765.1"
                     /translation="MKRYSVLVIIVAMASLAFGTAAFGQAYIGFVYPAGAQQGTTTQI
                     RLGGQRIDIPVGAIVTGTGVEAKLVEYRWYLDNQQVAV"
     gene            complement(389..1756)
                     /locus_tag="PKI05_10970"
     CDS             complement(389..1756)
                     /locus_tag="PKI05_10970"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HOA52766.1"
                     /translation="MNKNNHYNEPKVSRREILQQGIYGAGGVLLGKSLLSQVRAQEAA
                     PAPSTPPKIEPKAKSVIQIFLWGGMSHNDTWDPKPKSGRDYLGEFTEVIDTNVPGIQL
                     SSLFPKLARQADKFSLIRSMTHGNNGHETAAYIMQTGHLPGERLAYPSIGAVFAYFKG
                     KEYKGIIPPYVVLTRSPGRFSEEGFLGPNFKSFATGGDPNATRFEVEGVVARGITDDR
                     QKARRDLLEKLNTFGRLVVDHPEIALAEENKKAAYELILGPGRDVFDLSKEDPKLRDA
                     YGRHTFGQSCLVARRLVEAGVPYIVINYPGGWDTHSNHFTTMRRQCPQLDEGLAMLLQ
                     DLHDRGLLETTLVWCTGEFGRTPKVDWQPPWNGGRNHYGKVFTVLVAGGGFKGGRVVG
                     ASNELAEEVAERPVYPVDLLGSIYALAGIDYTAKLPHPEGLEARVLPEPGDRVKSNGL
                     LTEII"
     gene            complement(1780..>2536)
                     /locus_tag="PKI05_10975"
     CDS             complement(1780..>2536)
                     /locus_tag="PKI05_10975"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HOA52767.1"
                     /translation="ENPWFTRCIVNRIWVWIMGRGIIHEPDDIRDDNPPSNPELLSYL
                     EKELIQSGYNLRHLYKVILTSSTYQLTSIPRKPSPEVEANFGYYVPRRLDAEILIDAL
                     NKITGATDLYTSAIPEPYTYIPRDLTAVSLADGSVISPFLTLFGRSARSTGAFNERSN
                     KPTPSQWLYMLNSSQVYNKIATSQKLGELFRSGRPVPELIEEVYLTVLSRRPSEEEMT
                     IAREYIEKSRSRGEAAMDVVWALINTEEFLYRH"
BASE COUNT          541 a          739 c          665 g          591 t
ORIGIN      
        1 tcacggccac ttgctgatta tcgaggtacc agcggtactc gacgagcttg gcttcgacac
       61 cggtacccgt aacgatggcc cccactggga tgtcgatccg ctgtccgccc aagcgaattt
      121 gcgtggtcgt cccctgctgg gccccagcgg ggtaaacgaa tccaatatat gcctgaccaa
      181 aagctgcggt gccgaacgcc aggctggcca tcgccacaat gataacaaga acactatatc
      241 ttttcataaa gtgggttccc ttctccatcg catgtcactg gagttacgaa acgccgccgc
      301 ttgtgtattt cgaggctcca agccatcgac cctcgcgggg tagaccccgt ccggcttcgt
      361 caccactgaa gctcggtggg cgatgggctt agatgatctc ggtaagcagc ccattcgatt
      421 tgacacggtc acccggctcg ggcaggacac gggcctctaa accttcgggg tgcgggagct
      481 tggccgtgta atcgattccc gccaaggcgt aaatgctccc caaaagatcg accggataga
      541 cgggccgttc ggcgacctct tccgctaatt cgttggaggc tccaacgact cgaccgccct
      601 tgaagccgcc gccggcaacc aacacagtaa atacctttcc gtaatgattt cgtccgccgt
      661 tccatggtgg ttgccaatcg accttgggcg tccgcccaaa ctcgcccgta caccagacca
      721 aggttgtctc cagtaggcct cggtcgtgca ggtcttggag cagcatggcc aagccttcat
      781 cgagctgtgg gcactgccgc cgcatggttg tgaagtgatt gctgtgcgtg tcccacccac
      841 cgggatagtt gatgacaatg tacggaacgc ctgcctcgac caatcgccgg gccacaagac
      901 agctttggcc gaaggtatga cgtccgtagg cgtcgcgcag cttgggatcc tctttgctca
      961 gatcaaacac atctcgacct ggtcctaaaa tcagctcata ggccgctttc ttattctctt
     1021 cggccaacgc gatctccgga tggtcaacga ctaatcgacc gaacgtgttc aatttttcga
     1081 gtagatcgcg gcgcgccttt tggcgatcat ctgtgatacc gcgtgccacg acgccttcaa
     1141 cttcaaaccg ggttgcattg ggatcgcccc cggtggcaaa agacttgaag ttcggcccca
     1201 ggaaaccctc ctccgaaaag cgtcccggtg accgtgtcag gaccacgtag ggcggaatga
     1261 ttcccttgta ttcctttccc ttgaagtaag caaaaaccgc cccgatgctg ggataggcca
     1321 accgctcgcc tggcaaatgg cccgtctgca tgatgtatgc agccgtttcg tgcccattgt
     1381 tgccatgcgt catgctccgg ataagtgaaa acttatccgc ctgacgtgcc aacttgggaa
     1441 agagagaact caattgaatg cccgggacgt tggtgtcaat gacctctgta aactcgccca
     1501 ggtaatccct gccggacttc ggtttggggt cccacgtgtc attatgggac atacctcccc
     1561 aaaggaagat ctgaatgacc gacttggcct ttggttcgat tttcggagga gtcgacgggg
     1621 ccggtgccgc ctcttgtgcc cgcacttgcg agagtaatga ctttccgagg agtacccccc
     1681 cggcaccata gatcccttgc tgcagtatct cccgccgaga gaccttcggc tcattgtaat
     1741 gattgttctt gttcatggct tggtcctccc tctgtatcgt caatgtcgat ataggaattc
     1801 ttcggtgtta attaatgccc agacaacatc catcgcagct tcgcctctcg atcgcgattt
     1861 ttcaatatat tctcgtgcaa tggtcatttc ctcttctgac ggacgtcgcg acaacacagt
     1921 caggtaaacc tcctcgatca gttccggcac cgggcggcca gaacggaaca actctcctaa
     1981 cttctggctg gtagcaatct tgttataaac ctggctcgaa ttcagcatat acaaccattg
     2041 ggacggcgtt ggcttattgg agcgctcatt gaaggccccc gtggaacgag ctgaccgtcc
     2101 gaatagggtc agaaaaggac tgatgacact tccgtcggcc aacgacaccg ccgtgagatc
     2161 tcgcggaatg tatgtataag gctcagggat ggcgctcgtg taaagatcgg ttgctcccgt
     2221 gatcttgttc agggcgtcga tcaggatttc cgcatcgagt cttcgcggta catagtaccc
     2281 aaaattcgcc tcgacctccg gcgacggttt gcgagggatc gacgtcaatt gatacgtgct
     2341 ggacgtgaga atcaccttgt aaaggtgcct caggttgtac ccactttgga tcaactcctt
     2401 ttccagatag gacaaaagct ccggattgct gggcgggtta tcgtcacgga tgtcatccgg
     2461 ctcgtgaata atcccgcggc ccatgatcca cacccaaatg cggttgacaa tgcagcgagt
     2521 aaaccaggga ttctct
//
LOCUS       DAOPTR010000587         2533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_32561_length_2533_cov_3.300646, whole genome shotgun sequence.
ACCESSION   DAOPTR010000587 DAOPTR010000000
VERSION     DAOPTR010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32561_length_2533_cov_3.300646"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2533
                     /locus_tag="PKI05_10980"
     CDS             <1..>2533
                     /locus_tag="PKI05_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HOA52768.1"
                     /translation="LFDNQGRVKLGDFGLARRATDEHGSLLKGTTKYMAPELISAQFG
                     PVGPASDLYSLGFTAYELICGPDFELLFPTLSTFGRDKQIAWMMWHASADQKLPPIHR
                     VLEGVPDDLAEVVDRLVQKNQQDRIPSAAVALQRLKRPAAEVPPPPVSEEASEEELAE
                     RKRKRATRMIAIAAVACSLVISALMLWPSQKQSPPPAVSVEPLSGRVENVLLDARLLI
                     VDRGALGKEEIEIRAWDEVFVNDKKSLLRDLQPGDQIKITVLRDDQGRQIRRIEALRP
                     ERFAGRIEEMSPEMGTLSVTGSPGEPKLVVRVPNTAAIRLNGNETIDGKTLGLADLAV
                     GDRVELEYQRSENELIALKVDAYRVMRSKGVVEVVDASKQSIRLALSDSGKTLTLPWA
                     PDCVVTVNGLTAIGGQLQKPTDLLPNDQVEIEHDDQIRRIDAYRVLGVAGVIQQVHFD
                     ANAIDVQLTGDGKVAQFLVPDTCTITIAGSPATIVDLRPGDQVDIQHDTPGTTTGRAL
                     KVAAIRPADQTRFALLIGVEEFDDQNIPAARGIVEGVRRLADVLIQRGAFPRNQTLVL
                     TNPSRVRVEQAVSSFLKNVPGNADVVVYYAGYAAKDQEGRVFLLCKESHLQQLATSAL
                     SVQFLADSLEQASAKSKILLVDVSPEKAKASGQAFVSAEEALKAVQGPPGMAPFRTVT
                     AIAACAEGQEAMLAGDGTVFGAALIEAYQGKADENRDGRIEAGELYGYLSRQVPQLAQ
                     DKQRPRIFLPDNRPPRLSEEAKTAIRQLAAMVLQENLDSRVVNDIYRTALKLAGREPE
                     PRVLYALIQIKMGQTADAQRVLETLKLERPRELVAYLGLAWVKLVR"
BASE COUNT          585 a          661 c          759 g          528 t
ORIGIN      
        1 gctttttgac aaccaaggcc gagtgaaact cggggatttc ggcttggcgc gccgcgccac
       61 ggacgagcat ggcagcctcc tcaaagggac tacaaagtac atggccccgg agctgatcag
      121 tgcccagttt gggcccgtgg ggcctgccag cgacctctat tcgttgggat tcacggccta
      181 cgagttgata tgcggtcccg acttcgaatt actttttccc acattaagta cctttggtcg
      241 ggataagcaa attgcctgga tgatgtggca tgcctcggct gaccaaaaac tcccgccaat
      301 ccatcgcgtc ttagaagggg tacctgacga cctagccgaa gtcgtcgatc gtctcgtgca
      361 aaagaatcag caagacagaa tcccctcggc ggcagtagcg cttcagcgac tgaagcggcc
      421 agcagccgaa gtaccgccac cccccgtttc tgaagaggcc tccgaagagg aattggctga
      481 gcggaaaagg aaacgtgcga cacggatgat tgccattgcc gcggtcgctt gttctctcgt
      541 catctcggca ttgatgctgt ggcccagcca aaagcaatcc ccacccccag ccgtcagcgt
      601 ggagcccctt tccggtcgtg tggagaatgt cctgctggac gccagattgc tcattgttga
      661 ccgaggagcc ctaggcaaag aggaaataga gattcgcgct tgggacgaag tctttgtcaa
      721 tgacaaaaag tcgttactgc gtgacctcca accgggtgac cagatcaaga taaccgtcct
      781 gcgcgacgat caggggcgac aaattcggcg gattgaagct ttgcgtcctg aacgatttgc
      841 cggacgcatt gaagagatgt ccccggaaat gggaacacta agcgttacgg gtagtccagg
      901 ggagcctaag ctcgtcgtgc gagttcccaa caccgctgca atccgcttga acggcaacga
      961 gacgattgac ggcaaaacat tgggccttgc ggatctcgcc gtgggggacc gggttgaact
     1021 ggaatatcaa cggtcggaaa acgagttaat tgccttaaag gttgacgcat atcgggtgat
     1081 gcgatccaag ggtgtcgtgg aggtcgttga tgcttccaaa caatcgatcc gacttgcctt
     1141 gagcgattct ggtaagacgc ttacattacc ctgggcccct gattgtgtcg tcacagtcaa
     1201 cggccttacg gccattggtg ggcaacttca aaagccgaca gaccttctgc ctaatgatca
     1261 ggtggaaatc gagcacgatg atcagattcg gcgcattgac gcctaccgtg tgttgggggt
     1321 ggcgggggtt atccagcaag tccatttcga cgcaaacgcc attgatgttc agttgacggg
     1381 cgatggcaag gtggcgcagt ttttggtgcc ggacacatgt accattacaa tcgcggggtc
     1441 gccggccacg attgtcgacc tacggccagg ggatcaggtg gatattcagc acgatacgcc
     1501 cggcacaacc acagggcggg cactaaaggt tgccgcgatc cgacctgctg accaaacacg
     1561 ctttgcgctg ctgattggcg tggaagagtt tgacgatcaa aacatcccgg ccgctcgtgg
     1621 tattgtggag ggtgtaagac gtttggccga cgtgctcatt cagcgggggg cgtttccgcg
     1681 gaatcaaacg cttgttctga cgaacccgag ccgcgttcgc gtggaacagg cggtcagttc
     1741 gtttttgaaa aacgtcccgg gtaatgcgga tgtcgtcgtt tattacgccg gttacgcggc
     1801 aaaagaccaa gagggccgcg tttttctttt gtgcaaagaa agccatctgc aacaacttgc
     1861 cacgagcgct ttgtccgtcc aatttcttgc cgatagcctt gaacaagcga gcgcgaaaag
     1921 caagatcctg ctagtggatg tttctccgga gaaagccaag gcatcgggac aggcgtttgt
     1981 ctcggcggag gaggccctta aagcggtgca gggaccgccg ggaatggccc cgtttcggac
     2041 agtgactgcc attgctgcgt gtgccgaagg ccaagaggcg atgctagcag gggacgggac
     2101 tgttttcggc gcagccctga tcgaggcata tcaaggaaag gccgacgaga atcgagatgg
     2161 acggatcgag gcgggagaac tttatggtta tttatcacgg caggtgcccc aattggcgca
     2221 agataagcaa aggcctcgaa ttttcctccc tgataatcga ccaccgaggc tctctgagga
     2281 ggccaagact gctattcgac aactggcagc catggtgctt caggaaaacc tcgactcccg
     2341 agttgttaac gacatttatc gcaccgcgtt gaagctagcg ggcagagaac cggagccacg
     2401 ggttctatac gcgctgatcc agataaagat gggtcaaacc gcagatgccc aacgggtgct
     2461 cgagacactg aaactggaac ggccgcgaga attggtcgct tacctggggc tggcatgggt
     2521 gaaactggtg agg
//
LOCUS       DAOPTR010000588         2533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_32566_length_2533_cov_2.848668, whole genome shotgun sequence.
ACCESSION   DAOPTR010000588 DAOPTR010000000
VERSION     DAOPTR010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32566_length_2533_cov_2.848668"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="PKI05_10985"
     CDS             <1..779
                     /locus_tag="PKI05_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOA52769.1"
                     /translation="EIGFDLVELPVFDLDNDANYKKWAKRLDELGLARTATAIRTAEE
                     NLISSDPAVRRKGIEANKRNLDCCAMLGAEIMAGPFHSALGVFSGRGPTEDEWKWAVE
                     GMREVAEYADKVGVTLAVEYLNRFECYFLNTAADGARFCRDVNHPRCKMMYDTFHSHI
                     EEKNIPQAIRALKDYLVHVHISENDRSTPGTGNVRWPETFDTLHEIGYNNTMVIEAFG
                     LALDKLVPATKIWRRMYESERQLATDGLRFMKTEVAKRWK"
     gene            1129..2019
                     /locus_tag="PKI05_10990"
     CDS             1129..2019
                     /locus_tag="PKI05_10990"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOA52770.1"
                     /translation="MFARKPRGFTLVELLVVIVIISVLVALLLPAIVGSRESARRAQC
                     QHQLSQLGQALQTYESAKGHLPGYRNSFGNVPGLSWVAVLLPNIGRQDLWKSLRMGDF
                     SANGFPTPAVGLQLAEVTCPSNPRPGEYCALGYAANCGILDGTTMPNPLPNGPPQPED
                     ASHGVFFNHDSSLIPVANQIKVRTDRIPDGAQRTIAVSENLQAVSWMSFDSSNLPNAV
                     EVEVGILWADSPGQCGRINQCLKNPPPLPRPSSMHPGGVNILFCDGHVEFQADDVDYL
                     VYQHLMTPDSKKAGLPPEQP"
     gene            complement(2289..>2533)
                     /locus_tag="PKI05_10995"
     CDS             complement(2289..>2533)
                     /locus_tag="PKI05_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52771.1"
                     /translation="DTWSNHYEKDGIDQDGVRGADTGTDGFDNNNNGLVDEFDEWEAP
                     PPYPAPCRGIQVTIRVFEPDSRQLRQVTIVQDFLPK"
BASE COUNT          535 a          694 c          716 g          588 t
ORIGIN      
        1 aggaaattgg cttcgacctc gtggaattgc cggtctttga ccttgacaac gatgccaact
       61 acaagaaatg ggctaaacgg ctggatgaac ttggattggc ccgcacggcg acggccattc
      121 gcacggcaga ggagaacctt attagcagtg acccggccgt tcgtcgtaaa gggattgagg
      181 ccaacaagcg gaacctagac tgttgcgcca tgcttggagc cgagatcatg gccggcccgt
      241 tccactcggc tttgggcgtt ttttccggac ggggacccac cgaagacgaa tggaagtggg
      301 ccgttgaagg aatgagagag gtggccgagt acgccgacaa ggtaggcgtc accttggccg
      361 tggagtacct caaccgcttc gagtgctact tcctcaacac tgccgctgac ggcgcgaggt
      421 tttgccgtga tgtcaaccat ccgcgctgta agatgatgta cgatacgttc cattcccaca
      481 tcgaagaaaa gaatatcccc caggccatcc gagcactgaa ggattacctt gtccatgtcc
      541 atatctcgga aaatgaccgt agcacccccg ggaccggcaa tgtccgctgg ccggaaacct
      601 tcgacacctt gcacgagatt ggatacaaca acacgatggt cattgaagcc tttggcctgg
      661 ccctcgataa actcgttccc gctacgaaga tttggcgaag gatgtatgaa tcggaacgcc
      721 aactggccac cgatgggctt cgattcatga aaaccgaagt cgccaaacgc tggaaatagt
      781 cgtaaagcct ttcccataat ctccccttac gcattcagcg ggagaggggg aggagacggg
      841 cgtgtctcga atcagtgccg gttttctcaa ccgcttccga cggttgcctg ccctcacccc
      901 aaccctctcc cggagggaga gggggccgtt ttggcatagg ctcctaggtg ggattttggc
      961 gaagatgctc attggaagag agtgaagcca cgggcctgcc ttctccctgg tccgccaatc
     1021 ggggtttttc ccggcgcgcg gatgtggata gaataaaagg gacgaagggc aagtctgttt
     1081 ttgcgcacta cgggccttgt tccttttcaa gaagagagga gttgtgtgat gtttgcccga
     1141 aagccccggg gttttaccct tgtcgaattg ctcgtcgtga ttgtgatcat ctcagtcctg
     1201 gtggccttgc ttcttcctgc catcgtcggc tcacgagaat ccgcccggcg tgcccagtgt
     1261 cagcatcaac tatcgcaact gggccaggcg ttgcaaacgt acgaatcggc caaggggcat
     1321 ctgccaggct accgcaatag ttttggtaat gtcccaggcc ttagctgggt ggcggtgctg
     1381 ttgccgaata tcgggcggca ggatttgtgg aagtctttaa ggatgggaga tttttcagca
     1441 aatggttttc cgacaccggc cgtcgggctc caacttgcag aggtcacatg ccctagcaat
     1501 ccccgcccgg gtgagtattg cgccctaggt tatgccgcca actgcggcat cctcgatggg
     1561 accacaatgc ctaatccttt accaaacggt cctccccaac ccgaagacgc ttcccatggt
     1621 gtcttcttta accacgactc aagtctcata cctgttgcca accagattaa ggtccggacg
     1681 gaccgtattc ccgatggtgc ccagcgaaca attgccgtga gcgagaacct tcaagctgtt
     1741 tcctggatga gcttcgatag ctccaatctt ccaaatgctg ttgaggtcga ggtggggatc
     1801 ctttgggcgg atagcccggg acaatgtgga cgaattaatc agtgtctgaa aaatccgccg
     1861 cccttgcctc gcccgtcgag tatgcacccg ggtggagtga acatcctttt ctgcgacggc
     1921 catgtggagt ttcaggcaga tgatgttgat tacttggtgt accagcacct catgacccct
     1981 gatagcaaga aggctggcct gccacctgag caaccttgaa gacttagagg ctatcccgta
     2041 tcctcccctc cctgttcggc gggagaaggg gcgggggtta gtgtgtcccg aatcgctgcc
     2101 ggttttctca atggattcgg acggttgctt accctcaccc caaccctctc ccggcgggag
     2161 agggggattt tttggaaaag agtctcagat atcttcagac gctagaagaa ctgccgcgtg
     2221 ggtccgacag atgagctcct tcgctggttg tcggttatct ttgcaattct tgattgggtc
     2281 gtgaagggct atttcggcag gaagtcctgc acaatggtga cctgacgaag ctgccggcta
     2341 tccggctcaa agacccgaat cgtgacctgg attccccggc aaggggccgg gtaaggcggg
     2401 ggtgcttccc attcgtcgaa ttcgtccacc aggccattgt tgttattatc gaatccgtca
     2461 gtaccggtat cggctccccg cacgccgtcc tggtcaatac catctttttc atagtggtta
     2521 ctccaggtgt cgt
//
LOCUS       DAOPTR010000589         2530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_32618_length_2530_cov_2.915556, whole genome shotgun sequence.
ACCESSION   DAOPTR010000589 DAOPTR010000000
VERSION     DAOPTR010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32618_length_2530_cov_2.915556"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..1446)
                     /locus_tag="PKI05_11000"
     CDS             complement(124..1446)
                     /locus_tag="PKI05_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOA52772.1"
                     /translation="MGYVELEQKIRDKTARVGVVGLGYVGLPLLRAFIRAGFSAIGFD
                     VDETKVQKLNAGQSYIGHIPSQWIREWIEMRRFEATADMGRLNEPDAILICVPTPLNE
                     SRDPDLQYIEGTTAAIARVLRPDQLVVLESTTYPGTTREVMLPVLEKTGLKCGQDFFL
                     AFSPEREDPGNPNFSAERIPKVVGGIEPRSTQLAVALYSHAVVQVIPVASAEVAEACK
                     ILENTYRAVNIAMVNELKVLFQRLGIDIWEVIDAAKTKPFGFQAFYPGPGLGGHCIPI
                     DPFYLSWLARRHGMTTRFIELAGEINTSMPEYVVRRVIDALNEHGKPLKGSKVCILGM
                     AYKKDVDDPRESPSFALMEMLLAGGAIVTYNDPHIPKLPKMRHFRVPEMTSSELTAEF
                     LASQDCVLIATDHSAYDYDFIVRHAPLVVDTRNATKNVKIGREKIRKA"
     gene            complement(1687..>2530)
                     /locus_tag="PKI05_11005"
     CDS             complement(1687..>2530)
                     /locus_tag="PKI05_11005"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOA52773.1"
                     /translation="ANNAFLLTAYNERLFVSSGRRDIYGSDHHVRWMWSTRSVNSITV
                     DGVDQTPHSAATRGEITAFYTSPEVDCVEGEAGSCYLDPKAGPQGDRRLLDRFTRSIV
                     FVKPDWIVVYDRLVARRPVTYQYWLHSPEKMEVIGNGLVARVGKVECPVQFLVPQNLK
                     FHQTNEYDPNPRPRVKIREWHLTAETVNPERSVEFLTVYHPYRVGSASPGEVKLEMQS
                     GGYVLTITLGDGKAVLLLPKTDSAKMALGPLTTVGRVLVSRVNASGKEVRRVAVEGIS
                     SPAP"
BASE COUNT          596 a          738 c          624 g          572 t
ORIGIN      
        1 caggcaaact gaccgcggag gttggcccca gcaatcctag ggagctcgcg cggccaacgt
       61 agctcgcaga ggcaaggggc aggtatattc gagaagcttc tcaagcttcc ggcttaaacc
      121 cgcttatgcc ttgcgaatct tttcgcgtcc gatcttgacg tttttcgtgg catttcgagt
      181 atcaacgacg agcggcgcgt gtcgcacgat gaagtcgtaa tcataggcag agtgatccgt
      241 ggcaataaga acgcaatcct gagaagccag gaattctgcc gttaactcgg aacttgtcat
      301 ttctggcacc cggaaatgcc gcattttcgg aagcttggga atatggggat cgttgtaagt
      361 cacaatggcc ccgcccgcga gaagcatctc catgagggca aatgaaggac tttcccgcgg
      421 atcatccaca tccttcttgt aggccatccc cagaatgcac accttgctcc ctttcaacgg
      481 cttgccatgt tcattgaggg catcgatgac ccggcggaca acgtattccg gcatggacgt
      541 attaatctcg cctgcaagct cgataaagcg ggtcgtcatt ccatgccttc tggcgagcca
      601 actgaggtaa aacggatcga tggggataca gtgaccacca agtccgggcc cggggtagaa
      661 cgcctgaaaa ccgaacggct tagtcttggc ggcatcaatc acttcccaga tgtcaatccc
      721 caatcgttgg aaaaggacct ttagttcgtt gaccatcgca atgttaactg cccgataggt
      781 gttttcgaga atcttacaag cctcagccac ctctgcagaa gccaccggga tgacttggac
      841 aacggcatgc gagtaaagcg ctacagccaa ttgcgtactc cgcggttcaa taccgccaac
      901 aaccttagga atccgctctg ctgagaaatt gggattgccg gggtcctcac gctcggggct
      961 aaatgctaag aagaaatcct ggccgcattt taagcccgtc ttttcaagaa ccggtaacat
     1021 gacctcccgc gttgtccctg gatatgtggt gctctcgagg acgacgagct gatcgggcct
     1081 caggaccctt gcaatggccg ccgtagtgcc ttcgatatat tgcaagtcag gatcgcggct
     1141 ttcattgagc ggggtcggga cgcagatcaa aatcgcgtcc ggttcattca gtctccccat
     1201 atcggcggtg gcctcaaaac gccgcatttc aatccattcg cggatccatt gggacgggat
     1261 atgtccgatg taactttgac ctgcattcag tttctgaacc tttgtttcat ccacgtcgaa
     1321 accaattgcc gagaagcccg cacggataaa tgcccgcaaa agaggcaaac cgacgtaccc
     1381 caacccaacc accccaacac gtgccgtttt gtcccggatt ttttgttcca attccacgta
     1441 tcccatgcgt aaacactcct ttccagggcc gtaaccgccg tctcacatcc cccctcaccg
     1501 atcatcgggg gtgccgtgcg atttccccga cccagtcaat gctttggtcc cacgacacta
     1561 aaaaatgtag cttaattttt tcgaaagaag aggtcgccat cccctcctaa ttaggggaac
     1621 atcctctcaa gacggccttt tcccctgatt cgagcgttcg aaacaagagg aattcaaaaa
     1681 catcaattat ggcgcaggcg atgaaattcc ctcaaccgca acacggcgga cctctttccc
     1741 actagcgttt acgcgagaaa caagcactcg ccctacggtc gtcaacggtc cgagagccat
     1801 tttggcgcta tcggtctttg gaagaagcaa tacggctttg ccatccccga gggtgatcgt
     1861 aaggacgtaa cctccgctct gcatttccaa tttgacctcc cctggcgatg ccgatcccac
     1921 gcgataaggg tgatagactg tcagaaactc gacactgcgt tccggattta ccgtttcggc
     1981 cgtcagatgc cattctcgaa tcttgacgcg aggacgagga ttaggatcgt actcattcgt
     2041 ttgatgaaac ttcaagtttt gcggaaccag gaattgcacg gggcactcca ctttacccac
     2101 cctggcaacc aacccgtttc cgatgacctc catcttctcg gggctgtgca gccaatactg
     2161 gtaggtcaca ggtcttcgcg caaccaatcg atcatagaca acgatccaat ctggcttgac
     2221 aaagacaatc gatcgcgtga accgatccaa gagacgccga tctccctgcg gccctgcctt
     2281 cgggtcgagg taacaactgc ccgcctcgcc ctcgacgcaa tcgacctcgg gcgaggtata
     2341 aaaggccgtg atttcacctc gcgttgccgc ggaatgcggg gtttggtcaa ccccatccac
     2401 cgtgatactg ttcaccgaac gggtgctcca catccatcga acgtgatggt cacttccgta
     2461 gatgtcgcgt cgtccactgg acacgaacaa cctctcattg taggccgtta acagaaaagc
     2521 gttgttggcg
//
LOCUS       DAOPTR010000590         2526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_32695_length_2526_cov_3.618373, whole genome shotgun sequence.
ACCESSION   DAOPTR010000590 DAOPTR010000000
VERSION     DAOPTR010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32695_length_2526_cov_3.618373"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PKI05_11010"
     CDS             <1..225
                     /locus_tag="PKI05_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HOA52774.1"
                     /translation="HQANLRIIKAAAETIGIPEEKLYNNLDRYGNTSSASIPICLDEA
                     VRYGPLERGDLVLLSGFGGGLSWATGILRW"
     gene            487..1278
                     /locus_tag="PKI05_11015"
     CDS             487..1278
                     /locus_tag="PKI05_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52775.1"
                     /translation="MKAWRLVALVAVIVAGMTYASETSPPSLPAFKVESRVDLGEAEK
                     AVTSSITIFSRTKVFDFLEKPAEVTIIDWRDQRVVLLNPLHHEQTELSFDTIFRFQER
                     VQARALQDKDPRQRFFANPAFTIRMDSTEKELVFASNWLTYRVKVSDTVDPALIREFL
                     RFSDVMAQLNCLLVKGSRLPHPRIKVNKTLLERNLFPEEVILINGPRNFFEWLPGRRS
                     IVRSTHHLVLELTPQDRARLTQAEEWTQTFRRVNFIDYQAKLAAE"
     gene            complement(1318..2103)
                     /locus_tag="PKI05_11020"
     CDS             complement(1318..2103)
                     /locus_tag="PKI05_11020"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOA52776.1"
                     /translation="MIRERMTNGRQLSLGRLFIGILCATFFVGSWAALVRGESQSSAQ
                     PSSPTKSAEEKKESATSPYKWRDLFDGKSLKGWKVPKFGGEGEVRVQDGCIVMERGDP
                     MTGIAWEGEFPKVNYEVEYVAKRTDGIDFFATVTFPVGDTFCSFVTGGWGGSVIGLSS
                     INFNDASDNETTQFMVFKDNQWYKFRVRVTKVKIEVWIDDKKVVDLVIGDRRISTRYE
                     VDLCKPFGFASWCSTGVIRSIRCRELTPEEVKAIEEEAEKTRL"
BASE COUNT          596 a          718 c          604 g          608 t
ORIGIN      
        1 caccaagcca acttgcgaat tattaaagcc gccgcggaaa ccattggcat tcctgaggag
       61 aaactctata acaatctcga tcgatatgga aacacatcct cggccagcat ccccatctgc
      121 ctcgacgagg ccgttcgata cggccctctc gaacgcggtg acctcgtcct cctaagcggg
      181 ttcgggggcg gtctctcttg ggccaccggc atcttgcggt ggtgatcagc accggcgtgc
      241 aaagctcttt gatctagaaa aattgtgccc tcacattgtc tgaggtccgt tccacttgct
      301 caattgagcc cgtttttgcc gcgtgcgata ccaggtccgg aaaatggctg ccacatcatt
      361 ccacatattg gaatagtctt ggccagaagc agccaagcca atatgatgaa gggctaaagt
      421 caccccaagg gtcacggcaa ttgattgttc cttaacggcc tgtaccgcat acggaggtaa
      481 cttggcttga aagcgtggcg actcgttgcg cttgtcgctg taattgttgc gggcatgaca
      541 tatgctagcg aaacatcccc gccaagtctg cccgctttta aggtcgaaag ccgggtcgat
      601 ttgggtgagg cagaaaaggc tgtcacttcg agcataacca tcttttcgcg gacaaaagtg
      661 ttcgattttc tagaaaaacc cgcggaagtg accatcatcg attggaggga ccaacgtgtc
      721 gtcctactca accccttgca tcatgagcaa accgaactga gttttgacac tatttttcga
      781 tttcaagagc gcgttcaagc ccgtgcactg caggacaagg acccacgcca acggtttttc
      841 gccaatcctg ccttcactat ccggatggac tctacagaaa aggagttggt ctttgccagt
      901 aactggttaa cctatcgagt aaaggtatcc gacacagtcg acccagccct catacgggaa
      961 tttctgcggt tctcggatgt gatggctcag ctcaattgcc tcttggtgaa ggggtctcgc
     1021 ctgcctcatc cgcggataaa ggtcaacaag acccttctgg aacgaaacct ctttccggaa
     1081 gaggtcatcc tcatcaacgg tccaagaaat tttttcgaat ggctacccgg tcgtcgttcg
     1141 atcgtgcgga gcactcatca tctggtgttg gaattaacgc cccaagatcg tgcacggctc
     1201 acgcaagccg aagagtggac gcagacattc cggcgcgtca acttcatcga ctatcaggcg
     1261 aagctcgcgg cagaataagc cgggactcgc ggtagcgctt tttggtggcc taaccgttca
     1321 caagcgggtc ttctctgcct cttcctcgat ggctttgact tcctccggtg tcaattcccg
     1381 acatcggata ctgcgaatca cgcccgtgga gcaccacgaa gcgaaaccaa acggtttgca
     1441 gaggtcaact tcataacgag tggaaatccg tcgatcgccg ataactaaat caaccacctt
     1501 tttgtcatca atccagacct caattttcac ttttgttacc cgcacgcgga acttgtacca
     1561 ctgattgtcc ttgaacacca taaactgggt tgtctcgtta tcagaggcat cgttgaagtt
     1621 aatagatgaa aggccgataa ccgacccacc ccatccccct gtgacaaagc tacagaaggt
     1681 gtcaccgacg ggaaacgtca ccgtggcaaa gaaatcgatc ccatctgtcc ttttggcgac
     1741 atactcgacc tcgtagttca ctttggggaa ttcaccctcc catgcgatac ctgtcatggg
     1801 atccccgcgc tccatgacga tacacccgtc ttggacgcga acctcaccct caccgccgaa
     1861 cttgggtacc ttccagcctt tcaatgactt accgtcgaaa aggtcccgcc acttataggg
     1921 agaggttgcc gactctttct tctcctcagc ggatttcgtc ggagaagagg gctgggcaga
     1981 agattgcgac tcacctcgca ccagtgcagc ccaagacccg acaaaaaagg tcgcgcaaag
     2041 aatgccgatg aaaagtcgtc cgaggcttaa ttgacgccca tttgtcattc tctcacgaat
     2101 catcgtcaac ctccgtcgct cttgcgttac actaatcctc ctcgggaaac taatccgtcg
     2161 cgagcctgtt cggcgacttt attatgtcac gactcacttc ttcagtgaag catgacaagc
     2221 cgatttcagt cagcaatcat cccgctgtga attaccctgg cctgccttgc gttgatccaa
     2281 tcccgcacaa gggctgttct tgcgtattct ccaaccaggt gaggcgatgg tgggcgtccc
     2341 tccatactgt aggatcacgc ggcgccttct tccttcaaat ctcatacgct ttcggtggtc
     2401 gaaatctttt gctaccaacg gaagatacgg cgtgaacgct gctccttttc gtcaaatgtg
     2461 ggaaactcta tgatgtccag tggaaacatc gtgaccacgt tctgtacgga ttgtagaagg
     2521 attcgg
//
LOCUS       DAOPTR010000591         2526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_32698_length_2526_cov_3.400648, whole genome shotgun sequence.
ACCESSION   DAOPTR010000591 DAOPTR010000000
VERSION     DAOPTR010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32698_length_2526_cov_3.400648"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="PKI05_11025"
     CDS             <1..1115
                     /locus_tag="PKI05_11025"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52777.1"
                     /translation="PPLQAELSSPWDLCVVGEWVYIAMAGCHQIWRMDTALTGIGPYA
                     GNAREDIVDGPLLPPSAYALGFASFAQPSGLATDGEWLYVADSEGSSIRAVPLQPRGE
                     VRTIVGTSRLLAARLFTFGDRDGPPDQVLLQHPIGVCYGNGFLYVADTYNNKIKRIDP
                     RTGTTMTLVGDRQAGDTNDPPRFDEPAGITFARDRLFVADTNNHAIRVIELQPTLKVT
                     TLALKGLEPPPAVEEKGTFPEDKVDLRQEPVVRLRPGGTIKLQILFEFLAGFGLNPNV
                     PTDLVIERLSDQGVDSAGDKQVVRIPPGEGPLEVSLTGLNSNVKSLRIVCTYFWCEKS
                     GRGLCRIGTVGWLVPVQWADDGEDSIVLKHRVGGGS"
     gene            complement(1160..2095)
                     /locus_tag="PKI05_11030"
     CDS             complement(1160..2095)
                     /locus_tag="PKI05_11030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOA52778.1"
                     /translation="MVQFPVSIVDLPKTGVLTDDPSPCPQMDSAACPQMRHRPAGNVQ
                     LPPGAETSQVGESPDPRLTLPAPVSLLGLPIVPWRHYEVIHVIDWLVGRGEPSYFVTA
                     NLHYARLSATIPALKAVNDHAAFIVADGMPLVWLSRWRRKPLPERITGADLIWSMCEL
                     AANRGYRVFLLGGAPGVAETAGQRLQARLRGLQIAGIAAPCLDTLTPLERTRLITQIR
                     QARPDILFAAFGQPKGELWLAEHIQELGVPVAVQIGAALDFVAGRVRRAPRFVQRMGL
                     EWAYRTLQEPRRLLPRYLADGWFFLCQLMRSSPRS"
     gene            complement(2248..>2526)
                     /locus_tag="PKI05_11035"
     CDS             complement(2248..>2526)
                     /locus_tag="PKI05_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOA52779.1"
                     /translation="ARGENIWTRDELRRVAGGLATPRMAADVDIPLTQRESEVLKQLA
                     NGLTNKEIAQILNISYETVKEHVQHILRKIGVTHRTQAAVWAVKNRLV"
BASE COUNT          524 a          734 c          762 g          506 t
ORIGIN      
        1 ggccaccctt gcaggccgaa ttgagcagcc cgtgggacct gtgcgttgta ggagagtggg
       61 tctatatcgc tatggccggt tgccaccaaa tttggcggat ggacactgca ttgacgggga
      121 tcggtcccta tgccggcaat gcccgcgaag atatcgtcga tggtccgctc ttgccgccct
      181 cggcgtacgc tctgggattt gcctcgtttg cccagccgag tgggctggcg accgatggcg
      241 agtggcttta cgtggcggac agtgagggca gttcgattcg ggctgtgcct ttacagcccc
      301 gcggggaggt tcgcaccatt gttggcacat cgcgacttct cgccgctcgt ttgttcacct
      361 tcggggatcg cgatgggccg cccgatcagg tcctcctgca acatccgatt ggcgtttgtt
      421 acggcaacgg atttctgtat gtggccgaca cttacaacaa caagatcaag cggatcgatc
      481 cccgcacggg aaccaccatg accttggtgg gtgataggca agccggtgat accaacgacc
      541 caccgcggtt cgacgaaccg gctggaatca ccttcgctag ggatcgcctt tttgtggccg
      601 acacaaacaa ccacgccatt cgtgtgatcg agcttcagcc caccctaaaa gtcacaacgc
      661 tcgcgctcaa gggcctggag ccgccgcccg ccgtcgaaga aaaaggcaca ttccccgagg
      721 acaaggtcga tcttcgccaa gagccggtcg ttagacttcg ccccgggggc acgataaagc
      781 ttcagatcct ctttgagttt ctcgcgggct ttggcctcaa tccgaatgtg cctaccgatc
      841 ttgtcatcga acggctcagt gaccaaggcg ttgattcagc cggtgataag caggtcgttc
      901 gcatcccgcc aggggaaggt ccgctggagg tgtctctcac gggtttgaac agtaacgtca
      961 agtccctgcg gattgtgtgt acctattttt ggtgcgaaaa gtctggccgg ggactttgtc
     1021 gcattggaac agtgggttgg cttgtccccg tgcagtgggc tgatgacggc gaagattcca
     1081 tcgtgctcaa gcatcgcgtg ggtggaggat cataagccaa atcatctctc gggagccgag
     1141 gtcgtaaaag agagtcgtgt caggaacgcg gcgaagaacg catcaactgg cagagaaaaa
     1201 accacccgtc ggccagatag cgcggtagca atcggcgggg ctcttggagt gtacgatagg
     1261 cccattccag ccccattcgt tgcacaaacc gcggagcacg acggacccga cctgccacaa
     1321 agtcgagggc ggccccgatt tgaaccgcga cgggaacacc caattcctga atgtgctcgg
     1381 ctagccacag ttcacccttt ggttgaccaa aagcggcgaa gagaatatcg ggtctcgctt
     1441 gtcggatttg ggtgatcagg cgagttcgct caagaggggt tagtgtatcc aaacatgggg
     1501 cggcaattcc ggcaatctga agaccgcgca accgtgcctg aaggcgctgc ccggccgttt
     1561 cggccacccc cggtgcccca cccaaaagaa aaacgcggta tcccctgttg gccgccagtt
     1621 cacacattga ccagatcagg tcggcaccgg tgattcgctc cggtaggggc tttcgccgcc
     1681 accgcgagag ccacaccagc ggcatgccgt ccgccacgat aaaagctgcg tggtcattca
     1741 cagccttgag ggccgggatc gttgccgaca accgagcata gtgtagattt gccgtgacaa
     1801 aatatgacgg ctcgccgcgc cccaccagcc aatcaatgac gtggatcact tcataatggc
     1861 gccaagggac aatcggcaat cctagaagcg agaccggcgc tgggagcgtc aatcttgggt
     1921 cgggagactc acccacctgg gatgtttctg cacccggggg cagttggaca ttaccggccg
     1981 gccgatgtct catttgaggg cacgcggccg aatccatttg agggcaggga gacggatcgt
     2041 cggtcagcac accggtcttg ggtaaatcga cgatcgacac gggaaactgc accatgacca
     2101 tcctccacac actctaaggg gccaaggagg acagaagacc ccatcgttcc ccgtagaaac
     2161 ttggcataca gactccgccc gtgcggagaa gatcggtctc gcgcgacccg aatggtgggg
     2221 caaaatcggg caaaggatgc ggggtattca gactagacgg tttttgacgg cccaaactgc
     2281 cgcctgcgtc cgatgggtga cgccgatctt ccgcaagatg tgctggacgt gttccttgac
     2341 agtctcgtag ctgatgttga ggatttgggc aatctcctta ttcgtcaggc cgttggccaa
     2401 ctgtttgagg acctcgcttt cccgctgagt gaggggaata tccacgtcgg cggccatccg
     2461 tggggtggcc agcccccccg ccacacggcg gagttcgtcc cgcgtccaga tattttcgcc
     2521 gcgggc
//
LOCUS       DAOPTR010000592         2526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_32700_length_2526_cov_3.153784, whole genome shotgun sequence.
ACCESSION   DAOPTR010000592 DAOPTR010000000
VERSION     DAOPTR010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32700_length_2526_cov_3.153784"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PKI05_11040"
     CDS             <1..602
                     /locus_tag="PKI05_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HOA52780.1"
                     /translation="AMESVLELPRSLMNYVRVPSYDDLPPGPLATTRLDPLLLELGLA
                     LPYQLTGRSEEEDEAEKEARRRQQRRGFVEDRPRVLTLAEKLELLFRYDFPDVFDIRI
                     TPVWAAGELLEYGDDFNKYITSKSLQKQEGIIFRHLLRLILLLGEFKQICPVELTPEV
                     WQAQLDELIVRFSEVCRRVDPTSTEQAMEQVESESVIDD"
     gene            819..1181
                     /locus_tag="PKI05_11045"
     CDS             819..1181
                     /locus_tag="PKI05_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52781.1"
                     /translation="MAQPDRSPHQPVETPSEAPQSPAGCLLRLYWMIGGYLIAVLCGV
                     SIVNHHGDFSLVDVIYWLAIIGILAARWVDIKRFSGTRADGQLATMRDWQTHAIVTVV
                     AGIIGWIVIHFVRWQGLW"
     gene            complement(1195..1788)
                     /locus_tag="PKI05_11050"
     CDS             complement(1195..1788)
                     /locus_tag="PKI05_11050"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOA52782.1"
                     /translation="MPLLLRDSKQQVDLLAVLFIALALAMDAFAVSLAVGLRLEQITA
                     RHTFRIAFHFGLFQFLMPILGWAIGRQFSLLISTWDHWLAFGILVVLGGKMIWQAFDE
                     ELTFTAEDPTRGWSLVGLSIATSVDALAVGVSLAVLAGSVWFPAVIIGLVAAFLSLIG
                     IHSGNWLGRRFCQWGEICGGGILIVLACRILLSHLAL"
     gene            complement(2119..>2526)
                     /locus_tag="PKI05_11055"
     CDS             complement(2119..>2526)
                     /locus_tag="PKI05_11055"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOA52783.1"
                     /translation="TCNHCPVAKAYEDRLIALQKDYQEKKVQVIALNVNNIPQDRLDQ
                     MKIRAKEKGFNFPYLYDPTQKTGHDYGAKVTPHVFVLDSERKIAYIGAIDDNMDPKQV
                     KRHYLREALDALLAGKTPETTMTKAFGCSIKYE"
BASE COUNT          610 a          691 c          661 g          564 t
ORIGIN      
        1 aggccatgga gagcgtcctg gaattaccgc gctcgctgat gaattatgtc cgtgtgccca
       61 gttatgacga tctccctccg ggaccactag cgaccacgag gctcgatccc ctcctcctgg
      121 aactggggct agcactgccg taccaattaa ccggtcgcag cgaggaggaa gatgaggcag
      181 aaaaagaggc ccgccgacgg caacagcggc gggggttcgt cgaagatcgt cctcgcgtct
      241 taacacttgc cgaaaaactg gaactccttt ttcggtacga tttccccgat gtttttgata
      301 tccgtatcac accggtttgg gcagccggtg aacttctcga atacggggac gacttcaaca
      361 agtacattac aagcaagtcc ctgcaaaagc aggagggtat cattttccgt catttgctga
      421 gactgatcct cctgctcggg gaattcaagc aaatctgccc agtggaattg actcccgaag
      481 tctggcaggc ccaattggac gagctgatcg tacgatttag cgaggtttgc cgccgcgtcg
      541 atccgacaag tacagaacaa gccatggagc aggtcgaaag cgaaagcgtg atcgacgact
      601 agccacagtt aacaacaatc aaggcgattc cccagcgtta cggaaactcc cttaggcatg
      661 ctttgttagc tcggttgttc ttgtcatacg gcctcaaagc ctttatcata ggatcaatca
      721 acgttcgaca gtgcaagggt gcagcatcta gctgcaggtg ttgcggatgg tcgggccgcc
      781 attcgttttt ttcttcgagt taatgaggaa ggaacgcgat ggcacaaccc gatcgttctc
      841 cgcaccaacc tgtggaaacc ccctcagagg cgccacagag cccggccggt tgtcttcttc
      901 gactctattg gatgatcggt ggctatctga tcgccgtctt gtgtggcgtc tcgattgtca
      961 accaccacgg tgacttttct ctggtggatg tgatttactg gttggccatt atcggcattt
     1021 tggctgcccg ctgggtggat atcaaacgat tcagcggcac ccgggccgac ggtcaattgg
     1081 ccacgatgcg ggactggcag acccacgcta tcgtaaccgt tgtcgccgga atcatcggtt
     1141 ggatcgtcat ccacttcgtt cggtggcagg gtctttggta acttgctggg tcaattacag
     1201 agccaaatgg gagaggagga ttcggcacgc caggacaatg agaatgccgc ccccgcagat
     1261 ttctccccat tgacaaaaac gtcggcccag ccaattacca ctgtgaatac cgatcaagct
     1321 gaggaaggct gcaaccagtc cgataatgac ggccggaaac catactgatc cggcaagcac
     1381 cgctagagat accccaacgg ccagggcatc gacactggtc gcaattgaca agcctacaag
     1441 tgaccacccc cgggttgggt cttcagccgt aaacgttagt tcttcatcga acgcctgcca
     1501 gatcattttg ccccccagga ctaccaggat gccaaaggct agccagtgat cccaggtgct
     1561 aatcaatagg gaaaactgtc ggccaatggc ccagccgaga ataggcatca aaaactgaaa
     1621 aagcccaaaa tgaaaggcaa tccgaaaagt gtgtcgagcg gtaatctgct ccagacgcaa
     1681 acccacagcc aaggatacgg caaaggcatc catggccagg gctaaggcta taaataaaac
     1741 ggccaataag tccacttgtt gcttggaatc tcggagtaat aacggcatca cggtacatgc
     1801 ggagaatacg gctccagccc cgactgggct attccttcca cgtataggca attatcccga
     1861 gctcgacaac gccctcaagc cgaattggca aaagtggagt cctcaatcac cagcatgcac
     1921 tcatctacgt tcacgggcgg aggaagccag ccctcggctc tgaaggcacc aagccgcccc
     1981 gctggcaaaa aataagcctc acatcgcttg ggggatgcga ggcaaaagat cattcggcca
     2041 attctgacat tctccctatc gggagaacaa cggggacagt taccggcctt tttcccggcg
     2101 cagtctgtgc cacaaagatc actcgtactt tatcgagcag ccaaaggcct ttgtcatggt
     2161 tgtctctggg gtcttgcctg ccaagagggc atcgagggct tccctcaggt aatgtcgctt
     2221 gacctgcttg ggatccatgt tgtcgtcgat ggccccaata taggcaattt tgcgctcaga
     2281 atcgagcaca aacacatggg gcgtaacctt tgcaccgtag tcgtgccctg tcttctgggt
     2341 cggatcatac aaatatggaa aattgaagcc cttctccttc gctcgaattt tcatttgatc
     2401 caatcgatct tgtgggatgt tgttaacgtt cagcgcgata acctgaacct tcttttcttg
     2461 gtagtccttt tgcagggcaa tgagccgatc ttcgtaggcc ttggccaccg ggcaatggtt
     2521 gcaggt
//
LOCUS       DAOPTR010000593         2519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_32821_length_2519_cov_3.020698, whole genome shotgun sequence.
ACCESSION   DAOPTR010000593 DAOPTR010000000
VERSION     DAOPTR010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32821_length_2519_cov_3.020698"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..1555)
                     /locus_tag="PKI05_11060"
     CDS             complement(161..1555)
                     /locus_tag="PKI05_11060"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOA52784.1"
                     /translation="MKHVWFVLALLVCLPVSAQDFEVGIGRVNITPDKPIRLSGYAAR
                     NKPSVGVLQEIWAKTLAIRDKEGHRIVIITTDLIGFDHDFSQNLFNEAKKRFGLNREE
                     ILLTCSHTHSGPVVGGNLRTMYKLTEEEAATIDEYAKQLHEKLLTAIDQALQDMQPAV
                     LRVGHGQAGFAINRRELRGDNVVLGVNPTGPMDHDVPVLTVSTPNGKLRAILFGYACH
                     NTTIGGTTGEDFYKIHGDYAGVAQAELEQALPGVMAMFTILCGADQNPNPRGAVDYVV
                     QHGKSLAAAVQQVIQGPMEEVKGPIRAAVAEAELEFAPHTREMFQEELEKALAAKDIY
                     REGRARRMLELYDKGQPMRSLALPLQVVRFDKSLTILAIGGETCVEYALRCKREYPHE
                     NLVVIGYANEVMCYIPSKHVLLGGGYEPVSSMIYYGMPGPFAENVEEVIMASIHKLLE
                     RVGLQPTGTHTAAN"
     gene            complement(1608..>2519)
                     /locus_tag="PKI05_11065"
     CDS             complement(1608..>2519)
                     /locus_tag="PKI05_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52785.1"
                     /translation="KESLNHFQVVTQVGFSAAQVDRVASNRRAWTPDGRLVTRYSSCK
                     DPQIRDLPEGLIDPLLRTVTFANADGPIVRLHYYATHPQSFYGDPRASSDVPGFARAR
                     LEKEEGVFQIYFTGCAGNVTMGKYNDGSREARDQLTERLYQAMVQAARTTKYQPLRSI
                     GWQSVPFRFVTRSDPGYREEDLRSRLANPSVDPVQRVRAACGLSFFQRAAKSLSLQAL
                     YLNDAVIVHLPGECFVEYQLYAQSLRPDAFVAVAAYGDLGPGYICIAKAFEEGGYEPT
                     ATYLAPENENILKETIATLLKVQRTKP"
BASE COUNT          587 a          741 c          622 g          569 t
ORIGIN      
        1 ccaagggcta gcgcgagcgc aatacaagac aataccgctc gcggggcgac ttggcgcctt
       61 ttcccgggta actgttggaa aaacatgcta aatcagtatg aagacaatga ttctgtccga
      121 atgattacaa cggtggtcag ccaatgctcg accgcagcac ctaattggcg gccgtgtggg
      181 tgcccgttgg ttgcaggcca acacgctcaa gaagcttgtg gatggaggcc atgatgacct
      241 cttccacatt ctccgcaaaa ggccccggca tcccatagta aatcatactc gaaactggct
      301 catacccacc accaagaagg acatgtttgg atgggatgta acacatcacc tcattggcat
      361 acccaatgac gacaaggttt tcatgaggat attcccgttt gcaacggagg gcgtactcca
      421 cacatgtttc cccaccgatc gccaagatgg tgaggctctt gtcgaaccgc accacctgca
      481 atggcaaggc taacgaccgc atcggttgcc ccttgtcata cagctccaac atgcggcggg
      541 cacggccttc tcggtagatg tcctttgcag ctaaagcctt ttccaattct tcctggaaca
      601 tttcgcgtgt gtgaggagca aactcaagct cggcttctgc gacggctgcg cggatcgggc
      661 cttttacctc ttccatgggc ccttgaatga cctgttgcac ggccgcggcc aatgacttgc
      721 catgttggac aacataatca acggcacctc gcggatttgg gttctgatca gccccgcaaa
      781 ggatcgtaaa catggccatc acacctggca gggcttgctc caattcagcc tgggcaacgc
      841 ccgcataatc cccgtgaatc ttgtagaaat cctcacccgt ggtcccacca atggtcgtgt
      901 tatggcatgc atagccaaaa aggattgccc gcagcttccc gttcggcgta ctaacggtca
      961 acaccggaac atcgtgatcc atcgggcctg tggggttcac ccccaggacc acgttatctc
     1021 ctcgcaactc tcgtcggtta atggcgaaac cggcctggcc atgacccacc ctcaacacgg
     1081 cgggttgcat gtcttgaagc gcctggtcga ttgccgtcag caacttttcg tgcaattgtt
     1141 tggcatactc atcaatcgtc gctgcctctt cctcggtcag cttgtacatg gtccgcaaat
     1201 tgccgccgac cactggacca ctgtgggtgt gggagcacgt caaaaggatt tcctcccggt
     1261 tgagaccaaa tcgcttcttg gcctcattaa acaaattctg cgagaagtcg tggtcaaatc
     1321 cgataagatc cgtcgtgatg ataacaattc gatgtccttc cttgtcccga atagccagcg
     1381 tctttgccca aatctcctgc aaaacaccca cggaaggttt attccgcgct gcgtaccccg
     1441 acaatcggat cggcttatcg ggtgtgatgt tgacccttcc gatcccgacc tcgaaatcct
     1501 gggcggacac aggaagacaa accaaaagtg ccaaaacaaa ccatacgtgt ttcatagttt
     1561 gttctcctct caacgaaccg ttgacgatgt acccccatgc gagttgatta tggttttgtc
     1621 cgctgcactt tgagcaacgt ggcaatcgtt tctttcaaga tgttttcatt ctccggagcc
     1681 aagtaggtag ccgtcggttc gtagccgccc tcctcaaagg ccttggcaat acagatgtaa
     1741 cccggcccca gatcaccata cgcggccacg gccacaaatg catctgggcg caaggactgc
     1801 gcatataact ggtactccac aaagcactcg cccggcagat ggacaatcac ggcatcattc
     1861 aagtaaaggg cttgtaggga taacgacttt gccgcccttt gaaagaatga aagcccacac
     1921 gcggcacgaa ctcgttgcac aggatcgaca cttgggttcg ccaaccggct acgcaggtct
     1981 tcttcccgat atcctggatc ggaccgagtc acaaagcgaa acggtacact ttgccagccg
     2041 atggaccgca agggttggta ctttgtggtt cgcgcggcct gcaccatcgc ttggtataac
     2101 cgctctgtta attgatcgcg ggcttcacga ctgccatcat tgtacttccc catcgtgaca
     2161 ttcccagcgc agccggtgaa ataaatctga aacaccccct cctccttttc gaggcgggcc
     2221 cgcgcaaaac ccggaacatc actactagcc cgcggatctc cgtaaaaact ctggggatgt
     2281 gtggcgtagt aatgaagccg gacaatcggc ccgtcagcgt tggcaaaggt cacggttctt
     2341 aaaagcggat caataagccc ttcgggtaga tcgcggattt gggggtcttt acatgagcta
     2401 taccgcgtca ccaaacggcc atcgggtgtc caagctcgcc gattcgaggc cacacgatcc
     2461 acctgtgcag cgctgaatcc aacctgagtc acgacctgga aatggttcag cgactcttt
//
LOCUS       DAOPTR010000594         2518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_32841_length_2518_cov_3.153471, whole genome shotgun sequence.
ACCESSION   DAOPTR010000594 DAOPTR010000000
VERSION     DAOPTR010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32841_length_2518_cov_3.153471"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="PKI05_11070"
     CDS             <1..705
                     /locus_tag="PKI05_11070"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HOA52786.1"
                     /translation="SIAETLYREGAELAYTHLPGPSNERRVRRLLEPYNPKVLVPCDV
                     QSDDDILNVFATLEENYEQLDFVVHAIAFAPPAELRNPYVETTRQGWHVAMDISAYSL
                     VAVTRAASRLMENGGAILTISYFGAEKVMPGYNLMGVCKAALEHSVRYLAWDVGPKRI
                     RVNAISAGPVRTLSSAGIAGFDEMQRHAAEKSPLRRNVELHELASTAVFLLSDASSGI
                     TGEIVHVDCGYNIVGL"
     gene            821..>2518
                     /locus_tag="PKI05_11075"
     CDS             821..>2518
                     /locus_tag="PKI05_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOA52787.1"
                     /translation="MQVLHVAGLRKFYGPEPVLDGVSFDVFAGDRAGLVGPNGTGKTT
                     LLKIIAGRESADGGECLVTSGVKLGYLEQHAEFPEGRTVWEEARTAVQHLIDLQHEAE
                     TTAARLAHTQDPTERRRLAARFDFLQQELHRLNAYHLDHRIEEVLQGLGLGPSYYDRP
                     AGQLSGGEQNRLLLAKLLLAEPDLMLLDEPSNHLDIEATQWLEEFLANHHAAMIIVSH
                     DRYFLDKVTNRTLELYRGTVDSYTGNFSAYLRQKEERLLVARRTYEKQREEIAKAEEF
                     IRRHHYGQKAAQAQDRRKKLERIELVEPPREIEPPPIRFPPAERSGDVVLRVENLGKG
                     FERPLFSGLTFDIARGERWGILGPNGCGKTTLLRCLLGEVLPDVGRVVRGYNVVIGYF
                     DQYLHCLPDDLPAMDAVLPTETVADDLIEQKRRDLLAKFGITGDRALTPVKNLSGGER
                     CRVALARLTATRANLLVLDEPTNHLDLWARDALEQALKQFDGTVLFVSHDRYFIDQVA
                     DHLIVFEADRVRVIHGNYSTYTALRAAQAEAKSLDADSRSGKDQADKNRPKPSQSEPS
                     "
BASE COUNT          542 a          783 c          703 g          490 t
ORIGIN      
        1 tccatcgccg aaaccctgta tcgggaaggg gccgagttgg catacaccca tctcccggga
       61 ccatccaacg agcggcgggt ccgccgtctt ctggaaccct acaaccccaa ggtccttgtg
      121 ccgtgtgatg ttcagtcgga tgacgacatc ctcaacgtat ttgcgacctt ggaagaaaac
      181 tacgaacaac ttgatttcgt ggtgcatgcc atcgcctttg ccccacccgc cgagcttcgt
      241 aatccttacg tcgagaccac acgtcagggc tggcatgtgg cgatggacat ttcggcctac
      301 agcttggtcg ccgtgacccg cgcggcaagc cgcttgatgg aaaacggtgg ggcaatcctc
      361 acaatcagct attttggggc agagaaggtc atgcccggat ataacctgat gggcgtgtgc
      421 aaggccgccc tggaacatag cgtccgctat ttggcttggg acgtcgggcc caagagaatt
      481 cgggtgaatg cgatcagcgc tggccccgtg cggacgctca gttccgcagg aattgcgggc
      541 ttcgacgaga tgcagcgcca tgccgcggaa aagagcccct tacgacgcaa cgtggagctc
      601 catgaattgg cctcgacagc cgtcttcctc ttgagcgacg cctcaagcgg aatcaccggc
      661 gagatcgtcc acgtcgattg tggctacaac atcgtgggat tgtgagggat ttcgcggccc
      721 atcctggccc ttccttaaaa cgtgcctttg ccgtgggctg ggcagaggac aaatggagac
      781 aacgccaaac gaacggcagt ccaagagatg ctgatgtccc atgcaggttt tgcatgttgc
      841 cggtttgaga aagttctacg gcccggagcc tgtcctcgat ggcgtctcgt ttgacgtctt
      901 cgccggcgac cgcgccggct tggtgggtcc caacggtacc ggcaagacga cactcctcaa
      961 gattattgcc gggcgggaat ccgccgatgg cggtgaatgt ctagtcacca gcggcgtcaa
     1021 actgggttac ttggaacagc acgccgagtt tcctgagggc cgcaccgtct gggaagaagc
     1081 acgcacggcg gtgcaacacc ttatcgattt gcagcacgag gcggaaacca cggccgcccg
     1141 actggcccac acccaggacc ccacggagcg ccgtcggcta gccgcccgct tcgatttcct
     1201 tcagcaggaa ttgcatcggc ttaacgccta tcacctcgac cacaggatcg aagaagtctt
     1261 acaggggtta ggactggggc cgtcctacta tgatcgtccg gccggtcaac tcagcggtgg
     1321 agagcaaaac cgattactgc tggccaaact cctcctcgcc gaacctgacc tcatgctcct
     1381 cgatgaaccc tcgaaccacc tcgatatcga agccacgcaa tggctcgagg aattcctggc
     1441 caaccaccac gcggccatga tcatcgtcag ccatgaccgc tatttcctgg ataaggtcac
     1501 caaccgaacg ctcgaactct accggggaac ggtcgattct tacacgggca acttctccgc
     1561 ctatttgcga cagaaggaag aacgcctcct cgttgcccga cgaacctacg aaaaacaacg
     1621 tgaagaaatc gccaaggcgg aagaattcat ccggcgtcat cactacgggc agaaggcggc
     1681 ccaggcccaa gaccgacgca aaaagttgga acgcatcgaa ttggtcgaac caccgcggga
     1741 aatcgaacca cctcctatcc gctttccgcc cgccgaacgt agtggcgacg tggtccttcg
     1801 ggtggaaaac ctgggcaagg gcttcgaacg cccactcttc tccggtttga cattcgacat
     1861 cgcccggggt gaacgctggg gtatcctcgg tcccaatggc tgcggcaaaa ccaccctgct
     1921 ccgctgtttg ctgggcgaag tgctccccga tgtgggccgt gttgtccgtg gctataacgt
     1981 ggtcatcggc tactttgatc agtacctcca ctgcctgccg gatgatctcc cggcgatgga
     2041 cgcggtcctt cccacggaga cggtggccga cgatctcatt gagcaaaaac ggcgcgatct
     2101 cttggcgaaa ttcggtatca caggcgaccg cgccctgacc ccggtgaaaa acctcagcgg
     2161 tggagaacgc tgtcgcgtgg ccttggcccg cctcacggca acccgcgcaa atctccttgt
     2221 cctcgacgaa cccacaaatc atctcgacct ttgggcgcgc gatgccttgg agcaagctct
     2281 caagcaattc gatggcaccg tcctttttgt gagccacgat cgttatttca tcgaccaggt
     2341 cgccgatcat ctgatcgtct ttgaggctga tcgtgtccgc gtcattcacg gaaattactc
     2401 gacgtacacc gcgttacggg cagcccaagc cgaggcaaag tccctggatg ccgattcccg
     2461 gtcgggaaag gatcaagccg acaagaatcg gcccaagccc tcccaaagcg agccctca
//
LOCUS       DAOPTR010000595         2514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_32907_length_2514_cov_3.937373, whole genome shotgun sequence.
ACCESSION   DAOPTR010000595 DAOPTR010000000
VERSION     DAOPTR010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32907_length_2514_cov_3.937373"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1534
                     /locus_tag="PKI05_11080"
     CDS             <1..1534
                     /locus_tag="PKI05_11080"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOA52788.1"
                     /translation="TYAWVVFALVAAWFERGRQRQNYVARLFTEEDRGGTPYGFRSGM
                     SSDDLQPEPHEQVILMQADRIGAACLPYLRVELSGPEWTTGPVAFDRCRGAVAAAGRV
                     FTFAGDSCWRSQPIESDWIADWLLTHLERLVDQDRSSPDPTFDHRLGEAVKAVAAVVA
                     SGQRLPPRPTPTSRGAYHDPERAKRLRAHLRELFLRSPKRPALLIALCEAAESLGTRE
                     DLQLLDEQSRHLDVQAGFSRHQTDQILQTRDRVLGREPLRSELLKKLLQRIESLGLEQ
                     LPVPEGRPLLFRRPTDGAEMVLITAGSFWRGDDHTEETSPKRRVHLSSYLIDVVPVSQ
                     ESFNLWAETQGRILRVDRGFFPVQGLPDDVPRDRPYASHVTWFAAQAYAQWAVVGGHL
                     PTEAQWEKAARGSEDDRRYPSGEVWNEEPVSPYGVRVCHLLEWTQDAFDPEAYRHNPV
                     VFDPRMESAAGAGDEALRVVRGRAPESSVASYNLATRLGMEPITGAFTAPVGFRVAVD
                     LE"
     gene            1545..>2514
                     /locus_tag="PKI05_11085"
     CDS             1545..>2514
                     /locus_tag="PKI05_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52789.1"
                     /translation="MSQTLVAKLRDLPLSQYILRGKSAIAAAQYEELRRLQEEQAAVT
                     PLESGPFRLFLSDSQESMLQEIGKDRRSLLASLNQGVDVVRVVPAKIGGGGLVIGFID
                     RGRLRIHLRHESEPPAPGDWRLLGVEVEVGMPEGPPLARLTARWSLFNRQDSDAVSGD
                     ADSVLKELRCTKKAYRAWIRSWKPPEIDAGSLVLHVSKKLQQHEASSDELLNDLFTGA
                     AKVFPVKRLSSEGRTRLLLFVPSHKVCLQLLPPDWPDAKAGHWVLHSLNRGVEKNDAV
                     VLLRVHWLSARQPYKTEGLSDDWLTQLAQAEQDYDDWKRLGWQPRHL"
BASE COUNT          482 a          711 c          825 g          496 t
ORIGIN      
        1 cacttatgcg tgggtcgttt ttgcccttgt cgcggcatgg ttcgagcggg gccgccagcg
       61 gcagaactat gttgcccgcc tgttcaccga ggaggacagg ggtgggacac catatgggtt
      121 ccgttcaggg atgtcgtcgg acgatttaca gcccgaacct cacgaacagg taatactgat
      181 gcaggcagac cggatcgggg cggcgtgtct tccctacctc cgggtagagc tatcggggcc
      241 agaatggaca acggggcccg tcgcattcga ccgctgccgg ggtgcggttg cggcggctgg
      301 ccgcgtgttc actttcgctg gcgattcgtg ttggcggagc cagcccatag aaagtgattg
      361 gatagccgat tggctgctga cgcacttgga gcgcctagtg gatcaggacc gcagcagccc
      421 tgacccgacc ttcgaccatc gacttggaga ggcggtgaag gcggtcgcgg ccgtggtggc
      481 aagtggccag cggctacctc cacggcctac gccgacttcg cgaggggctt accatgaccc
      541 tgaaagggct aagaggttgc gcgcgcacct gcgggagctc tttttacgtt cgcccaagcg
      601 cccggccctg ttgatcgccc tttgtgaggc cgccgaatct ctgggaaccc gtgaagactt
      661 acagttgctg gacgagcaat cccgccacct cgatgtccag gccgggttca gcagacatca
      721 aaccgaccaa atcctgcaaa cccgcgaccg cgtcttgggg cgagaaccgt tacggtcgga
      781 gctcctaaag aagttgcttc agcggattga atcgcttggg ctagagcagt tacccgtgcc
      841 cgagggaaga cctttgctgt tccgtcggcc cacggacgga gccgagatgg tcctgatcac
      901 ggctgggtct ttttggcgtg gcgacgacca caccgaagaa acctccccga agcgtcgggt
      961 gcatctgagc agttacctca tcgacgttgt gccagtatcc caggagtcct tcaacctctg
     1021 ggctgaaaca cagggccgta ttctgcgggt cgatcgcggc ttcttccccg tccagggtct
     1081 gcccgacgac gtaccgcggg accgcccata cgccagtcat gtcacatggt tcgcggctca
     1141 ggcgtatgcc cagtgggcgg tcgtgggagg tcacttgccg acggaggcgc aatgggagaa
     1201 agcggcgaga ggaagtgagg atgacagacg ttatccgtcg ggagaggtct ggaacgagga
     1261 gccggtttct ccttacggag ttcgcgtgtg ccatctgctt gagtggactc aggacgcatt
     1321 cgaccctgag gcctaccgcc acaaccccgt cgtattcgat ccccgtatgg aaagtgcggc
     1381 aggggcgggc gacgaggctc tacgggtggt ccgcgggcgc gcgccggaga gttccgtggc
     1441 cagttacaac ctggccacac gtctggggat ggaacccatt accggggcgt tcaccgcgcc
     1501 cgtagggttc cgcgtcgccg ttgacctcga gtaggaggta aggcatgagc cagacgctgg
     1561 tggctaagtt gagggatctg ccactttccc agtacatctt gcgcggtaaa agcgcgattg
     1621 ctgccgctca atacgaagaa ttgcggcggt tgcaggagga gcaggccgcg gtgacgcctt
     1681 tggagagtgg ccccttccgc ctcttcttgt ccgactcgca agagtcgatg cttcaggaga
     1741 tcggaaagga ccgcagaagc ttgttggcca gcctcaacca gggcgtggac gtggtccggg
     1801 tggtaccggc caagataggg ggtggcggct tggtgattgg cttcatcgac agggggcgac
     1861 tgaggatcca tctgcgacat gaatcggaac cgcctgctcc gggggattgg cgtctgctgg
     1921 gagtcgaagt cgaagtcgga atgccggaag gccccccgtt ggcccggctt acagcgcggt
     1981 ggtcgttatt taaccgtcag gattccgacg ctgtttccgg ggatgcagac agcgtgctca
     2041 aagagctccg ttgcacgaag aaagcgtatc gggcttggat caggagctgg aagccccctg
     2101 agatcgacgc gggatcgctt gtgctacatg tctcgaagaa actgcaacag cacgaagcct
     2161 cttctgacga gctactaaat gacctgttta ccggtgctgc taaggtcttc cctgtcaagc
     2221 gactctcgtc agaaggtagg acgaggttat tgcttttcgt gccatcccac aaggtctgcc
     2281 tacaactcct tcctccagac tggccggatg ccaaggcggg ccactgggtg ttgcactccc
     2341 tgaacagggg ggtggaaaaa aacgacgcgg tggtcttgct gcgcgtgcac tggctctcgg
     2401 cgcgtcagcc ctacaaaacg gaggggctaa gtgacgattg gttaactcaa ctcgcccagg
     2461 ccgaacagga ttacgatgat tggaagcggc tgggatggca accacggcac ttga
//
LOCUS       DAOPTR010000596         2513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_32933_length_2513_cov_3.779902, whole genome shotgun sequence.
ACCESSION   DAOPTR010000596 DAOPTR010000000
VERSION     DAOPTR010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32933_length_2513_cov_3.779902"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            399..1736
                     /locus_tag="PKI05_11090"
     CDS             399..1736
                     /locus_tag="PKI05_11090"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA52790.1"
                     /translation="MKSKATRRQFLGSSAAGVAAVWLGSQTAVTGQQAAPPSEIVNVG
                     LIGCGGEGRAVATAHSRLGDARIVAVCDVNSKRLADARQQFGGEKIAAYDDYRRMLDS
                     KDVDAVIVATNDHWHVLPTIHACQAGKDVYVEKPLSVFIAEGQAAVKAAEKYKRIVTI
                     GTQQRSWPHYQQAAEMIKEGKLGIITEVRVWDFDCMYPGFGNPPDSDPPPELNWDFWL
                     GPAPKVPYNPNRYNHFYWFFDYGGGWQVDWAVHHYQIVHWFLEAKGPISAAAMGGFYC
                     FENTNTEWPDSFVAICEYGPTPVAPKGFVMQYTFSGAARRQQRSHAKCFYGTKGSMII
                     DRSGFTITYEAGENRVPTEEFTVENVFKKETHVSSLQAHARVFVDCIKSRQKPPADVE
                     VGHQATIPGHLMNISWKVGRKIRWDPEKEEIIGDAEASSLLSKPYREPWKLEV"
BASE COUNT          573 a          696 c          701 g          543 t
ORIGIN      
        1 gggagataaa ctccgtgagt tgacggccaa gccgaattat caatggtttg agacagtgac
       61 tttgccccaa tatttgcaac atgttcccac caaaggctcg gtcgccttgg gacgtggtcc
      121 attgaccatt gaaccgttca aagggacgag gctgtagtcg ctccatgctc cacggggcgg
      181 aggagatgcg ccccaattct tcgtggcgag tttggcccaa tttgaccgag gcaatcggtt
      241 tgctaataat tgtggcacac cctcccagcg ggtcattttg acgaggacct cttgccacag
      301 gtcccgccag aacgttcggc cctgatgttt ggtgcggacg ttcgttgtca gcctgaaggt
      361 atgttcttta accgttgcca agaaggagga agaaccctat gaaaagcaag gcgacgcggc
      421 gtcaattcct cgggagttcg gcggctggtg tggctgccgt ctggttggga agtcaaacgg
      481 cggtgacggg tcagcaggcg gcaccgccca gtgaaatcgt caatgtcggc ttgattggct
      541 gcggaggtga agggcgggcc gtagccacgg cacattcccg gttgggcgat gcccgcattg
      601 ttgccgtctg tgacgtcaac tccaagcgtc tggccgatgc ccggcaacag ttcggtggag
      661 aaaagattgc cgcctacgat gactaccggc gcatgctgga cagtaaggat gtggatgctg
      721 tcattgtggc gaccaatgat cactggcatg tcttgccgac gattcacgcc tgccaggccg
      781 gtaaggatgt ctatgtcgag aagcccctca gtgtcttcat tgcggaaggg caggccgccg
      841 tgaaggccgc ggaaaaatac aagcggattg tgaccatcgg cacgcagcaa cggagttggc
      901 cccactacca acaggccgcc gaaatgatca aggaaggcaa attggggatc atcacggaag
      961 ttcgggtctg ggactttgat tgcatgtatc cgggctttgg caatccgccc gacagcgatc
     1021 cgccaccgga attgaattgg gacttctggt taggtcccgc gcccaaggtt ccctacaatc
     1081 ccaatcgtta caaccatttc tactggttct ttgattacgg cgggggttgg caggtggatt
     1141 gggcggttca ccactatcag atcgtccact ggttcttgga ggccaaggga ccgatctcgg
     1201 ccgcggccat gggtgggttt tattgttttg aaaacacgaa cacagagtgg cccgactcgt
     1261 tcgtggcaat ctgtgaatat ggtcccacgc ctgtggcccc gaaaggtttc gtcatgcaat
     1321 acaccttcag cggtgcggcc cgccgacagc aacggtccca cgccaagtgc ttctatggca
     1381 cgaaaggatc catgatcatc gaccgcagtg ggttcaccat cacctacgaa gctggcgaga
     1441 accgtgtgcc cacggaggag tttaccgtgg agaatgtgtt caaaaaggaa acgcacgtct
     1501 ctagcctcca agcccacgcc agggtctttg tcgattgcat caagagtcgg caaaagccgc
     1561 cggcggacgt ggaggtgggc catcaggcga cgatccccgg ccatctgatg aacatttcgt
     1621 ggaaagtagg acgcaagatt cgctgggacc ccgaaaagga agaaatcatc ggcgatgccg
     1681 aggccagcag cctattgagc aaaccctacc gagaaccgtg gaagctggag gtctgatcca
     1741 cccgtgggag aagtcagcga ctcttccggc tgattggtag aagccgtacc aaaaaaagcg
     1801 gacggcctca tgtcgaggcc gtccgatcgt tttcgtctta gtcgtctttt tagagaggac
     1861 ttctccggtg aaaccatcat ttggcggctg acgtgcccga gcacgctatc tcagtcggcc
     1921 gaaaaaaggc tgactcggtt tgccaaacgg cttttccctc gcggagggaa cagggtgaat
     1981 caactctttt cgggaccgac tgtccagatt taacgcctct cagaacaccg aaggtcctcc
     2041 cgggcagaaa gtctgtgtca ctttctcgat agaccctcct gtgccatttc accagaaaag
     2101 accacgggat cttcaccatt tgttcgggcg cccgactcca ccgccggacg cccgcgtgcc
     2161 aggatcagat cgcgctcgtg ctcggcacat aggctgtcct cccataaaga ggctaacgct
     2221 tttcgctcca gccttggcaa ccttccggtc gccacagtat acgatataca cacgcctgta
     2281 aaaaagtcaa acccaattca cgccaagggc aagcgtacca ttcgaattat ggcgtaagtc
     2341 attatcctga aataacataa gtcatatatt tctcgtcgca ttcgggtatc gaacgtaccc
     2401 attttcgaag gccatatacg aaaaacatga cctctcaacc gctacaggca ttcaccttta
     2461 cgcgaaccta cgaacgtgca tcttgcgacc tcttttcgcc tgccacacac tct
//
LOCUS       DAOPTR010000597         2507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_33042_length_2507_cov_3.001223, whole genome shotgun sequence.
ACCESSION   DAOPTR010000597 DAOPTR010000000
VERSION     DAOPTR010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33042_length_2507_cov_3.001223"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PKI05_11095"
     CDS             <1..589
                     /locus_tag="PKI05_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA52791.1"
                     /translation="GGGALMNQAIHSVDLLAWLMGPVVEIQAATGLLAHERIEVEDVA
                     LAILRFSNGALGVLEATTAAYPGHLKRIEIHGNRGSAVMEEEDIIKWDFAEEHPDDQV
                     IREKMASRISGGGGASDPAAIGHHGHARQFADVVRAIKEGTRPLIDGYEGRRSVEIIL
                     GVYQAAELGRVVKLPLPGDPDLVARKVGVANLRKV"
     gene            843..2171
                     /locus_tag="PKI05_11100"
                     /pseudo
     CDS             843..2171
                     /locus_tag="PKI05_11100"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
     gene            2270..>2507
                     /locus_tag="PKI05_11105"
     CDS             2270..>2507
                     /locus_tag="PKI05_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOA52792.1"
                     /translation="MDIELVVIGGGPGGYAAAFLAADLGMKVAIVDQEPRLGGVCLLR
                     GCIPSKALLHVTRVIAETEELKEWGVDYSKPAVEL"
BASE COUNT          552 a          644 c          763 g          548 t
ORIGIN      
        1 tggtggcggc gccctgatga accaggctat acatagtgtt gaccttttag cctggttgat
       61 ggggccggtc gtcgagattc aggccgccac cggactttta gcacatgaac gcattgaggt
      121 tgaggacgtg gccctggcta ttctcagatt cagcaacggt gcactggggg ttttggaggc
      181 caccaccgcg gcctatccgg gacacctcaa acggatcgaa atccacggta atcgtggttc
      241 ggctgtgatg gaagaagagg acattatcaa gtgggacttt gccgaggaac atcctgacga
      301 tcaggtgatt cgtgaaaaga tggcctccag gatcagtggg ggtggcgggg cgtccgaccc
      361 cgcggccatt gggcaccacg gtcacgcacg gcagtttgcc gacgtggtcc gtgccatcaa
      421 agagggcacc aggcccttga tcgacggtta cgagggtcgc cgttcggtgg aaattatctt
      481 gggggtatat caggcggcgg aacttggccg ggtcgttaaa ttgccgttgc cgggagatcc
      541 cgatcttgtg gcgagaaagg tcggagttgc gaatctgcgc aaggtttgag tcatgtcaag
      601 gagaattccc ttgacctagt gcgcaggaag ggacttcgtt gtccggccac cgcggggctt
      661 gccgcattga caataattgc aagtaagaaa cgtcaactcg tatttttcgt tcgttggcct
      721 ttggcacgag ttcgttatcc gatactgccg gaactttgca agcctctttg gtttacaaat
      781 ccgagaagta actgtgttgc gttgtggatg tgtctgccag cggatgataa ggatggatct
      841 ttatgccaat tgaatttgct cttccgaacc ttggcgaaaa tgtaacatcg ggtgacgtca
      901 tcaatgttct tgtcaaagaa ggggatgtca tcgtcggaaa ccaggcagtt atcgagttgg
      961 aaacagacaa agccgtggtg gaaatcccct gcccctatgg cggtcaagtg aaaaaagtcc
     1021 tcgtcaagaa gggggatacc gtcaaggtag gtcaaaccat tttgatcgtg gaaagcgagg
     1081 ttgcagagaa agctccggtc gtgtccccag ccaagggacc ggagtcacag gtggccgcgg
     1141 ccccgcccga agaaaaaccc agtccaccac ctgccgcccc gactccgcct cccgtagcag
     1201 cggtaacggc agcaacgaca gcaaccgctg tggctcccga accctcaccc ctagtggagg
     1261 tggacagccg tcttctcccc gctggcccgg cagcacggcg gctggcccgg gagttgggtg
     1321 tcaatttggc aacagttcag ggaaccgggc gtcatggtcg gatcacggtt gaagacgttc
     1381 aacgggccgc gcagagccgg gcagaggctc cccgtgtcac tattccgagt cccgtcgtcc
     1441 cgccaggaga gcctgggcaa gactcttggg gccctatccg ccgcgagaaa atgagcaaaa
     1501 ttcgcaagac catcgcggca cagatgacgc gatcgtggac aacgattcca cgggtcacca
     1561 atttcgacga cgccgacgta acggatttgg agaatttgcg gaagtcggtc ccgcccgatt
     1621 tcctaggtga agggttgaaa ttgactttca tgcccttcgt catgcgggca gtgagcctcg
     1681 ccctccgtca tcatcccctt ctaaatgcct cgctagatga ggaaaacgag cagatcatct
     1741 acaaggaata catcaacttg gggatcgccg tggatacccc acgtgggctg gtcgtcccag
     1801 tcgtgcgaaa cgcggatcag atgaccatac cgcagttggc acgggcactg agcgaagtgg
     1861 cacgaaaagc gcgaaacgta gagtttaccg tggacgaact gcggggtggg acatttacaa
     1921 taagcaacct cggtgctgtg ggctgaacgt acagcactcc aattatcaac tatcccgagg
     1981 ttgccatatt acttttgggt cggtcgcgtt ggttaccggt tgttcgcgac ggacagattg
     2041 tcgcccggta tatgatgccg cttagcctgt cctatgatca tcgtctggtg gatggtgcgg
     2101 cggccgcgcg attcctcaac gaaataatcg actggttaca aaaccctggg aaattgctcc
     2161 tggtcagtta gttttaagaa tggcgccaga gcttaacaga acaatttggc ggtgttgata
     2221 gcagacgcac cccattatcg gtgtgcggcg gtttggagac ggataaagca tggatatcga
     2281 gctcgttgtt attggcggtg gaccgggtgg atacgcggcg gcctttttgg cagccgattt
     2341 gggcatgaag gttgccattg tcgatcaaga accccgattg ggcggtgtct gtctgctccg
     2401 tgggtgcatc ccttcgaagg ccttgctcca tgtcacccgg gtgattgccg aaacggagga
     2461 gctaaaggaa tggggcgtcg attattccaa gcccgccgtc gagctta
//
LOCUS       DAOPTR010000598         2507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_33043_length_2507_cov_2.856036, whole genome shotgun sequence.
ACCESSION   DAOPTR010000598 DAOPTR010000000
VERSION     DAOPTR010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33043_length_2507_cov_2.856036"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="PKI05_11110"
     CDS             complement(<1..1161)
                     /locus_tag="PKI05_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52793.1"
                     /translation="MTTVHRRKTSWATKFVDRLAGWLITLGGIGTICAVVGVCVFLVW
                     VVLPLFKPARLIPIHFVAQSANESSGGSVAPSGNQTVPFDAKLLAVGLDETMTIIWSV
                     DEDGNLIVQQFADQDVLFKKDLSSHGRPRTLAVDTRSGYIAFGYDQGRIQFTRVHVAV
                     TPAQMEDVPIDIREQVTGKPPNKALSGEGECWFAQSDNVYRLDVRVEEEPDVTVGEAA
                     VDRLAFSLRAEVRNIAALTADGILHIGRTDKKVNFLTGEESVRFTGGALNLQDAGISA
                     IPDFLQISGVADSTYLIWRNGRTLRLDTRDLTNPRIAEEVSLLSPGEGEISAVSFLIG
                     KMTLIVGSTTGTVSAWFPIKPAHAETADGVHLVRAHTYRPGQGAVVALGVSHR"
     gene            complement(1234..2214)
                     /locus_tag="PKI05_11115"
     CDS             complement(1234..2214)
                     /locus_tag="PKI05_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOA52794.1"
                     /translation="MVTLKKICGALVLGILIGWWTPSVAQVQVDPQLPDYKPVQGISG
                     NLKSVGSDTMNNLMTLWAEGFRRFYPNVQIEIEGKGSSTAPPALIAGTAQFGPMSRPM
                     KSAEIDQFEARYGYKPVALVTAIDMLAVYVNKDNPLKGLTLQQVDAIFSKTRRGGYPH
                     DIRTWGQLGLTGVWSQQPISLYGRNSASGTYGFFKEHALFGGDYKDEVKEQPGSSSVV
                     QGVASDRYAIGYSGIGYKTADVRALPLAPDANSPFVPAEPDHAYTGDYPLARFLYVYV
                     NYKPGTTLDPLRAEFIKFIFSKQGQAEVIKDGYFPVPAVIAKKSLASVGL"
BASE COUNT          558 a          699 c          691 g          559 t
ORIGIN      
        1 tcggtgagaa acgcccaaag cgaccaccgc tccttggccc ggccggtacg tgtgtgctcg
       61 gacaaggtgg acgccatccg cggtctccgc gtgcgcgggt ttaatgggaa accatgctga
      121 taccgtcccc gtggtgctgc ccacaatgag cgtcattttc ccgatgagga atgataccgc
      181 actgatttcg ccttctcctg gcgaaagcaa cgagacctct tcggcaatcc gtgggttggt
      241 aaggtctcgt gtatcgagac gcaaggtacg tccgttgcgc cagatcagat aggtggagtc
      301 tgccacgccg gaaatctgaa ggaagtcggg gatcgcggaa atgcccgcat cttgaaggtt
      361 caacgcgcca ccggtaaacc gaacgctttc ctcgccggtc aaaaagttga ctttcttatc
      421 ggtccggccg atgtgcagaa tcccgtctgc cgtcagagct gcaatgttgc gcacctcggc
      481 ccggagggaa aaggcaagcc ggtccacggc ggcctcgccc acggtgacgt cgggctcttc
      541 ctcgacacga acatccaggc gatagacgtt gtcagactgc gcaaaccagc attcgccctc
      601 accgctgagg gctttgttag gaggttttcc cgtcacttgc tcgcggatgt cgataggaac
      661 atcctccatc tgtgcggggg tcaccgcaac atggacacgt gtgaattgga ttcgaccctg
      721 gtcatagccg aatgcgatat aacccgagcg cgtgtccacc gctaaagttc gaggcctacc
      781 gtgagaagaa aggtcttttt tgaagaggac atcctgatcc gcaaattgct gcacgattaa
      841 attgccgtcc tcatcgacag accagatgat cgtcatggtt tcatccagac ccacggccaa
      901 taattttgcg tcgaatggga cggtttggtt accggatgga gccaccgagc cgccgctcga
      961 ttcgttcgcg ctctgggcga caaagtgaat cgggatcaac cttgctggct taaagagggg
     1021 gagaacgacc cagacgagga aaacgcaaac gcccaccacg gcgcagatcg tcccgattcc
     1081 ccccaatgtg attaaccacc cggcgaggcg atcgacgaat tttgtagccc atgaggtctt
     1141 acgtcgatgg acggtagtca tgggagcgat cggatcctag tcaagaccag taggcccaga
     1201 tgattcagac gtatgctggt aacaacactc ggcctataga cctaccgagg ccagcgactt
     1261 cttggcgata accgccggca ccgggaagta accgtcttta ataacctcgg cctggccttg
     1321 cttactgaag atgaacttga tgaactcggc ccgcagggga tcgagcgttg tccccggctt
     1381 gtaattgacg taaacgtaca gaaatcttgc cagcggataa tcacccgtgt aggcatgatc
     1441 aggctcagcc ggtacgaagg gcgagttagc atcgggtgcc aacggcaagg ctcggacgtc
     1501 ggccgtttta tagccaatac cgctgtatcc aatggcgtaa cggtcactgg cgacaccctg
     1561 gaccaccgag gaactgcccg gctgctcttt tacctcatcc ttataatcac cgccaaagag
     1621 ggcgtgctcc ttgaagaaac cataggttcc cgaggccgaa ttacgtccat aaaggctgat
     1681 cggttgctga ctccacacac cggtcaaacc aagctgcccc caggtgcgaa tgtcatgggg
     1741 ataaccgcct cgcctggtct tggagaagat ggcatctacc tgctgtaagg tcaaaccttt
     1801 caaggggttg tccttgttca cgtaaacggc cagcatatcg atggccgtga cgagggcgac
     1861 cggtttatac ccatagcggg cctcgaattg atcgatctcg gcgctcttca tgggtcgact
     1921 catcgggcca aattgcgccg tgccggcaat cagagctggt ggggcggtcg atgaaccttt
     1981 gccttcgatt tcgatctgca cgttggggta gaatcggcgg aagccttctg cccacaacgt
     2041 catgaggttg ttcatggtgt ctgatccgac actcttcagg ttgcccgaaa tcccttgaac
     2101 gggtttgtag tccggcagtt gcgggtcaac ctgaacttgt gccaccgacg gtgtccacca
     2161 tccgataagg atgccgagca ccaaggcgcc gcaaattttt ttcaacgtga ccatacctcg
     2221 tctcctcttt atgcgtttcg cttggaaaag ccacccatct tgcgggttcg ttgccgaaat
     2281 cacgcagatc ggatttttgt gtgctcaccc atcatcgccc accgtttcag catgataccc
     2341 gagaaatgtt aaggatttgt taagagattg ttaaaaaacg gttagggcgt gcccggcgac
     2401 ttaagaaata tgttcgggta actccgcctt ttcactgctt cacattttgg cggtggaagc
     2461 cggaagtcca tccgttgacc gtgtttgtta cgccatttac agtgaga
//
LOCUS       DAOPTR010000599         2507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_33045_length_2507_cov_2.741436, whole genome shotgun sequence.
ACCESSION   DAOPTR010000599 DAOPTR010000000
VERSION     DAOPTR010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33045_length_2507_cov_2.741436"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1842
                     /locus_tag="PKI05_11120"
     CDS             <1..1842
                     /locus_tag="PKI05_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52795.1"
                     /translation="SSEINGMARLKWYEHLLLNPLNVPHETEEFTRLLHAGFTAKSSE
                     GFRFALRQARSKMDVPDASLPGDLETLWKSENNDPYKKLENILVTARKTCARTVEPLT
                     PAELNELAKQLNYVLTTNTIVGHTVNSRGSAMRMVALLHKMDRGAQWDLAEILMGVCD
                     KEFLDSLRTSAPKSGPAIAVAGVTGEVLRRIDTPAGTILVGGPGKNEYRLEEMKDVAA
                     IVDVGGDDVYLEGTVSVERPILIIIDLAGNDRYEGKLPGIQGSAIIGGSILVDCAGND
                     IYWGQDVAQGSALGGVGILVDMAGDDQYLGYRRVQGQAIGGVGILLDYGGRDDYRAAM
                     WAQGFGGPLGFGLIDDLDGDDHYYVGGRFYDSYPETPGYDGWGQGVGAGIRGAANGGI
                     GVLLEGNGDDVYEFDYFGQGGGYWLAIGLARDFAGNDQRLGATLNAYNRSNRTQQRFQ
                     RFGNGWGCHYAVGVLIDDLGNDSYDGSIMGTGFGWDLSVGYLLEMSGDDQYLARVGGV
                     QGQGAQASLGVLYDFEGKDVYRGTSQGYASSSISYHPYPTCGGNFSFLIDYGGEDEYG
                     SRVRNNAVSRRGMGGFVVDRPKQEELDAMHASAKTSISSPISNSSGQ"
     gene            complement(2031..>2507)
                     /locus_tag="PKI05_11125"
     CDS             complement(2031..>2507)
                     /locus_tag="PKI05_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52796.1"
                     /translation="GVRLHVVELPADALKEAPQALKDIFGSNAGAVIGFSENLVCMAF
                     GPNPVSILKNALDQSASEVAVDKIFEFSLAVKPLIEFVQVAASEEWGEEERQLAEQLL
                     ASAGTKDRVTTDGVLIENGSRVRIEFQEGVLKVLAQLVIKAVAQQGVGFAPGAMAP"
BASE COUNT          557 a          630 c          776 g          544 t
ORIGIN      
        1 agttccgaaa tcaatggcat ggcacgcttg aagtggtacg agcacctcct gctcaatccg
       61 ttgaacgtgc ctcatgaaac agaggaattt acgcgactgc ttcacgccgg ttttacggcc
      121 aaatcgagcg aaggcttccg ctttgcccta cgacaagcac ggtcaaaaat ggacgtgccc
      181 gacgcgagtt tacctggcga cctggaaaca ctatggaagt cggaaaacaa tgacccctac
      241 aagaagctgg agaacatctt ggtcaccgca cgaaagacgt gtgcgcggac tgttgagccc
      301 ctcacgcccg ccgagctgaa cgagttggcc aagcagctta attacgtgct gaccactaat
      361 acgattgttg gccacaccgt taacagccgc ggcagcgcca tgagaatggt cgctctgcta
      421 cacaagatgg atcggggcgc gcagtgggac ttggccgaga tcttaatggg tgtgtgcgac
      481 aaggagtttc tggattcatt gcgcacatct gctccgaaaa gcgggccggc aattgcggtt
      541 gccggggtca ccggcgaggt actgagaagg atcgacacac ccgcggggac cattctcgtg
      601 ggaggaccag gaaaaaatga atatcgcctg gaagaaatga aagatgtggc ggccatcgtc
      661 gatgtgggcg gggacgacgt ttatctggaa ggcacggtgt cggtcgaacg gccgattctg
      721 atcatcattg atttggcggg aaacgatcgc tacgagggaa agctgccggg tattcagggg
      781 tctgcgatca ttggtggttc gatcctggtg gattgtgcgg gaaatgacat ctattggggc
      841 caggatgttg cccaaggcag cgcgcttggc ggtgttggca tcctcgttga tatggccggg
      901 gatgatcagt acctagggta tcgtcgcgtc caagggcagg ccattggcgg tgtcgggatt
      961 ctcctcgatt acggtggcag ggatgactat cgggcggcga tgtgggcaca ggggtttggt
     1021 ggtccgctcg gttttggcct gatcgacgat ctcgatggag acgaccatta ttatgtgggt
     1081 ggccgattct acgactccta tcccgaaaca ccaggttacg acggctgggg ccaaggggtt
     1141 ggcgccggca tccgtggggc agccaatggt gggattggcg tcttgttgga aggcaatggc
     1201 gacgacgttt atgaattcga ctattttggt caggggggcg ggtactggct cgccattggc
     1261 ttggcgcgag attttgccgg gaatgaccaa cgactggggg cgacactcaa tgcgtataat
     1321 cgatccaatc gaacgcagca acgatttcag cggttcggca atggttgggg atgccactat
     1381 gctgtgggcg tcttgattga tgatctgggc aatgactcgt acgacggttc aatcatgggc
     1441 acgggcttcg gctgggacct ttctgtgggt tatctgctgg aaatgagcgg tgatgaccag
     1501 tacctcgccc gtgtgggtgg cgtgcaggga cagggtgcgc aagccagcct cggggtactc
     1561 tacgactttg agggaaaaga tgtttaccga ggcaccagcc aggggtacgc gtcgtcatcg
     1621 atctcctatc acccctatcc aacctgtggc gggaatttta gttttctcat tgattacggc
     1681 ggcgaggacg agtacggtag ccgtgtccgg aacaacgcgg ttagccggcg cggaatgggc
     1741 ggttttgtag tagatcggcc caagcaggaa gagttggacg ccatgcacgc cagtgctaaa
     1801 accagcatct cttcgccgat cagcaacagc agcggtcagt gaggccgatg tcgcttaatt
     1861 tgggaaggaa caggtgtttt tccatcttac agcctatctc atactcgccc ctctcccgga
     1921 gggagagggg ggacttattg ggataggctc taaataaaaa ggcctgctgg aaggaaatct
     1981 cagcaggctt cttctgtttt agagatattg gggcaaagac acacgtcgcg ttacggagcc
     2041 atcgcccccg gtgcaaaacc aactccttgt tgggcaaccg ccttgataac gagctgggcg
     2101 agaactttga ggacgccttc ctggaactcg atgcgaactc gtgaaccgtt ctcgatcaac
     2161 acgccatccg tagtgacacg gtctttggtg cccgcagacg cgaggagttg ctcggcaagt
     2221 tgccgctctt cctcgcccca ttcttcgctg gcagcgactt ggacgaattc aatcaatggc
     2281 ttgacagcca aagaaaactc gaaaatcttg tcaacggcca cctcactggc ggactgatcc
     2341 aaggcgtttt tcagaatgga cacgggattc ggcccaaaag ccatacaaac aaggttttcg
     2401 ctaaagccaa tgacggcccc ggcatttgag ccgaaaatgt ccttaagggc ctgcggtgct
     2461 tccttcagcg catcggcggg aagctcgacg acatgcagcc gaacccc
//
LOCUS       DAOPTR010000600         2504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_33085_length_2504_cov_4.360964, whole genome shotgun sequence.
ACCESSION   DAOPTR010000600 DAOPTR010000000
VERSION     DAOPTR010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33085_length_2504_cov_4.360964"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..1635)
                     /gene="groL"
                     /locus_tag="PKI05_11130"
     CDS             complement(4..1635)
                     /gene="groL"
                     /locus_tag="PKI05_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008684202.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOA52797.1"
                     /translation="MAKQLVFDQSARQALAQGVSKLSMAVKATLGPRGRNAVLDKGWG
                     SPKITKDGVTVAEDIDLEDPNENLAARLVREAASKTNDVAGDGTTTATVLAEAIFREG
                     LKMVAAGADAMALSRGIQKAVDAVVEAIKKMATPIEETNRKEIAQIATIAGNNDPSIG
                     AVLADAFMKVGKNGAITVEEGKQAETTVEFIEGMQFDRGFLSPHFVTNQDEQSCELEK
                     CYILVHEDKISNARALVPLLEAVAKTNRPLLIIAEDVEGEALATLVVNKLRGILSVCA
                     VKAPAYGERRKAMLGDIAILTGGKAIFKDLGINLESVKLSDLGQAKKVIVTADDTTII
                     GGAGSKEEIEGRCEQIRRELQTTTSDYDREKLQERLAKLSGGVAQIFVGAATETEMKE
                     RKFLIEDAKSATQAALEEGVVPGGGVALLRAAKALEKLELTGDEALGAKIVEAALEVP
                     LRQIAENAGQEGAVVVAQVRKLKKNEGYDAEKDQYVDMFEAGIIDPAKVVRVALQNAA
                     SVACLLLTTSCLVTEIPKKETDKKPGHNHDEDFDY"
BASE COUNT          513 a          723 c          639 g          629 t
ORIGIN      
        1 cgattaatag tcgaagtctt cgtcgtggtt atggccgggt ttcttgtccg tctctttctt
       61 ggggatttcc gtaacgaggc acgacgtggt taacagcaag caggctacgc ttgcggcatt
      121 ttgaagagcc acgcggacaa ccttggccgg atcaatgatg ccggcttcga acatatcgac
      181 gtactgatcc ttttccgcgt cgtacccttc attcttcttc agtttccgca cctgagcgac
      241 caccacggcc ccctcttggc cggcattctc ggcaatctgc cgtaacggca cttccagggc
      301 ggcctcgacg atcttagccc cgagtgcctc gtcaccggtc aactcgagct tttcaagggc
      361 cttggccgct cgcagcaagg ccacaccgcc accggggacg acaccttctt ccaatgcagc
      421 ctgggtagct gactttgcat cctcgatgag gaacttccgt tccttcatct ccgtctcggt
      481 cgcggcacct acgaagatct gggccacgcc acccgacaat ttagccaatc gctcttgaag
      541 tttttctcga tcgtaatcgc tggtggtcgt ttggagttca cgacggatct gctcacaacg
      601 accttcgatc tcctctttgc ttcccgcccc accaatgatg gtggtatcgt ccgccgtgac
      661 gatcaccttt ttggcttgac cgaggtcact gagtttgaca gactcgagat tgattcccag
      721 gtctttgaag atagccttgc cgccggtgag gatggcaata tcgcccaaca tagccttgcg
      781 gcgttcacca taggccggcg ccttcaccgc acacacactg agaatgccac ggagcttgtt
      841 gacaaccaac gtggctaggg cctcaccttc gacatcctca gcgatgataa gaagcggccg
      901 attcgtctta gcaacggcct cgaggagcgg aaccaatgcg cgtgcgttgg aaatcttatc
      961 ctcatgcacg aggatataac atttttccag ctcgcaactc tgctcgtcct ggtttgttac
     1021 gaagtgaggg gacaaaaagc cccggtcgaa ttgcattccc tcgatgaact cgaccgttgt
     1081 ttccgcctgc ttaccctctt ccaccgtgat ggcaccgttc ttcccaacct tcataaaggc
     1141 atcggccaac acggcgccaa ttgaagggtc gttgttcccc gcgatggtag caatctgggc
     1201 gatctccttg cggttagttt cctcaatcgg agtagccatc ttcttgatag cctcgacgac
     1261 ggcatcgacc gccttttgga tgcctcggct caaggccatg gcgtcggcac cggctgcgac
     1321 catcttgagg ccttctcgga agatcgcctc ggcaaggacc gtggcagtgg tcgttccatc
     1381 acctgccacg tcgttggtct tggaagctgc ctcgcggaca agtcttgccg ccaggttctc
     1441 gttaggatct tccagatcga tgtcttctgc gaccgtcaca ccgtctttcg tgatcttggg
     1501 ggagccccag cctttgtcta aaaccgcatt tctcccccgc ggtccgagcg tggccttaac
     1561 ggccatagac agtttgctga cgccttgggc cagcgcttgt cgtgctgact gatcgaacac
     1621 aagttgtttg gccactggaa gttcctcctt attgactctc aagagaacct taaggtatgg
     1681 ctcagcttcg aacgctgatc ttcgtgttac gactgaggta agttgtcaac ctaccacgcg
     1741 cggcctttct gacacccgtt ttctgcgacg ttttcattca gccgcgctcg cccaccattg
     1801 tgtcgagacc accggttaca aaagcggtgt tcagctaccg cccgtatgac agcccagcgg
     1861 tttgtcccgt taccctgagc aagcggtgtg ccagaatgga ttgaagatcc taagttgttt
     1921 acagatggac agttatgaag atgaccgaca ctcgatgcct atccgctgag tgattaatga
     1981 tgccaaaatg gcagaaccca ctttgaccaa accctaccgc gaggctaagg gatactttca
     2041 cgcggagtaa caactttcga ggccgcggga cgcccttttt ggaatttcct gaaaaaatgg
     2101 ctcgaagcgt ttgatttgtt ccgcgtatga cctagacttt cagtaggtcg tggtggccaa
     2161 actgattcgc cggatgccga ttggtgcaaa agttcttgga acgacgatca tctcttgaaa
     2221 agaattctta ttcatagaac gtcgggttcc gtccgccgtg atttcgccgg ccaaaaaacg
     2281 cgagtctgtg cgatttagca acttagatga agctccccca gaaggggaat aatgtttcga
     2341 ggcaatgtcc ccccgtggga cgtgtgctcg aggatatccg tatgatgact tggcgcttgg
     2401 catcgacgat cgaagaatgg gtcaaggctc tccggcttgt ttatcaagtt tatcaacaag
     2461 ctggggttat ccagtcgagt aagtaccaaa ttcgcgtcac gcct
//
LOCUS       DAOPTR010000601         2491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_33319_length_2491_cov_3.983580, whole genome shotgun sequence.
ACCESSION   DAOPTR010000601 DAOPTR010000000
VERSION     DAOPTR010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33319_length_2491_cov_3.983580"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1580
                     /locus_tag="PKI05_11135"
     CDS             <1..1580
                     /locus_tag="PKI05_11135"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA52798.1"
                     /translation="TDAISDHAVRFIREHVATARTSPFFMYVAYTAAHWPLHALEEDI
                     AKYRGRYDGGYEPIRRARFERLKQMGLISPNWELSPQVGDWDKVEHKEWEIRCMEVYA
                     AQIDRMDRGIGRILEALRETGQWENTIVFYLQDNGACQEAIGRTGTWRRPVEPSLPPI
                     ALDAIRTDVRPSQNRRGVPTLSGPFIMPGPEDTYISYGINWANVSNTPFREYKHFVHE
                     GGIATPLIVHWPAGLTRRGDLEHQPGHLIDLMATCVELAGAQYPTEFQGHKIIPMEGV
                     SLVPLFKGQPIKRKEPIFFEHEGNRAVRDGKWKLVAKENQPWELYDMEADRTEMHDLS
                     AQYPEVVARLAAAWDEWAKRANVLPLGAWRAETKDTFSTKTQFVLAPGAQLPRTEAPY
                     VVGKGLKITAEIQAVGTSGVIVAQGGSAHGYALYIQDGKPCFAVRHKNQLKTVVGSQP
                     LSQGPGVLSVTWSNDVVKILWNKTVLAEAKEIPPLTNMPQDGLQIGRDDNGLVGDYPE
                     ENAFSGKLGKVVIEVLP"
     gene            complement(1723..>2491)
                     /locus_tag="PKI05_11140"
     CDS             complement(1723..>2491)
                     /locus_tag="PKI05_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52799.1"
                     /translation="PRVGLSTAQVEKRTVLAIPLETQQLLKLRDWGYTIDTTAMPGWA
                     KPEGECDPAFANIRAGMGGIPIRYRFRVTRRSQANVVLGICESYWDRPQIRPLICLVE
                     GARPMVVDPIDRWGRHQPGTLLFTALDNDGDGWITVTIKPVPGARDQNPILNVIWVFS
                     GDQRPNLARVAKGELNEQALYYVDVGGVRDQPILESQDLSFPVELAANSSKKLTFYVA
                     CPGGSVVTPELTAWTTESLFQAAKDVWKNWSQSVTAR"
BASE COUNT          563 a          678 c          728 g          522 t
ORIGIN      
        1 acacggatgc catcagtgac catgcggtgc ggtttatccg ggaacatgtg gccactgcgc
       61 gaacgagtcc gttcttcatg tatgtggcgt atactgcggc ccactggccg ttacatgcct
      121 tggaggagga cattgccaaa tatcgtggtc gctacgatgg ggggtatgag ccgattcgcc
      181 gggcgaggtt cgaacggctg aaacaaatgg ggctaatctc tcccaactgg gaacttagcc
      241 cccaagttgg cgactgggac aaagtggagc ataaggagtg ggagattcgt tgcatggagg
      301 tctatgcggc tcagatcgac cgcatggatc gggggattgg acgaatcctt gaggccctgc
      361 gggaaaccgg tcaatgggaa aacacaattg tcttctacct acaagacaac ggtgcctgcc
      421 aggaagcgat tggccgaacg gggacatggc gtcgccccgt ggaaccttcg ttaccgccga
      481 ttgcgctgga tgcgattcgc acggatgtca gaccgtccca gaaccgccgc ggggttccca
      541 cattaagcgg ccccttcatc atgccgggac cggaggacac atacatttcg tacggcatca
      601 attgggcgaa cgtttccaac acgccgttcc gtgaatacaa gcactttgtg catgaagggg
      661 ggattgccac tccattgatt gtccattggc cggcaggcct aacgcgccgt ggcgatctgg
      721 aacaccaacc ggggcatttg atcgacctca tggccacctg tgtggagttg gccggggcgc
      781 aatacccgac cgagtttcaa ggccacaaaa tcattcccat ggaaggcgtg agccttgttc
      841 ctctcttcaa gggccaaccg atcaagcgga aggaaccgat cttttttgag cacgagggga
      901 atcgcgcggt ccgggatgga aaatggaagc tcgtcgctaa ggagaatcaa ccctgggagt
      961 tgtatgacat ggaggccgat cgcacggaaa tgcacgacct ctcggctcaa tatcccgagg
     1021 ttgtagctcg acttgcggct gcctgggacg agtgggccaa acgtgctaat gtcttgccgt
     1081 taggtgcttg gcgagccgaa accaaagaca cattctccac caagacccaa ttcgtgttgg
     1141 cgcccggcgc tcaacttcca cgaacggaag ccccttacgt tgtgggcaag gggttaaaaa
     1201 tcacggctga gattcaagcc gtgggtacgt caggtgttat tgtcgcccag ggaggctcgg
     1261 ctcacggata tgcgctttac atccaggatg gcaagccctg ctttgccgtc cgccataaga
     1321 accagttgaa aaccgtggtt ggcagtcaac ctttgtcgca gggaccaggc gtactctccg
     1381 taacttggtc caacgatgtg gtaaaaattt tgtggaataa gactgtccta gcggaagcga
     1441 aggaaattcc accgcttaca aacatgccgc aagacggcct gcaaattggt cgggatgaca
     1501 acggcttggt cggcgactat ccggaagaaa acgctttcag cggaaagctg gggaaagtgg
     1561 ttatcgaagt tctgccgtaa cctgaatgtt ggccacaatc cttccagttc gtagcgcggc
     1621 acataaccca cacaccgtgt gctatcggtg agtgtgtgcc accgacgccc acaagacgtg
     1681 acctgtgtca ggtctttcgg atgcagaaaa acgaccaatt ggttatctgg ccgtgactga
     1741 ttgcgaccag tttttccaga cgtccttggc tgcctggaac aaactttcag tagtccacgc
     1801 agtcagttcg ggtgtaacca ccgaaccacc gggacatgcg acgtaaaagg tcaacttttt
     1861 ggagctattg gctgccaact ctacggggaa acttaagtcc tggctttcca ggatgggttg
     1921 gtccctcacg ccgccgacat ccacgtaata cagtgcctgc tcgttaagct cgcccttggc
     1981 cacgcgagct aagttgggac gttgatcccc agaaaagacc caaatcacat tgaggatggg
     2041 gttttggtcg cgtgccccgg gaacgggctt gatcgttacg gtaatccagc cgtctccgtc
     2101 attgtcgagc gccgtgaata acagtgttcc cggctggtgg cggccccaac gatcgatggg
     2161 gtcaacaacc atggggcggg ccccctccac caggcagatt aaaggacgga tttgcgggcg
     2221 gtcccagtaa ctttcacaaa tccccaggac gacgttggct tggcttcgcc gtgttacccg
     2281 gaaccggtaa cgaatgggaa taccccccat tccggcgcgg atgttagcaa aagcgggatc
     2341 gcattcaccc tctggcttag cccaacccgg catcgccgtg gtgtcgatgg tgtaacccca
     2401 atcgcggagc ttcagcaatt gttgcgtctc gagggggatg gcgagaaccg tccgcttttc
     2461 gacctgggca gtagaaagcc cgacccgcgg c
//
LOCUS       DAOPTR010000602         2491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_33320_length_2491_cov_3.983580, whole genome shotgun sequence.
ACCESSION   DAOPTR010000602 DAOPTR010000000
VERSION     DAOPTR010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33320_length_2491_cov_3.983580"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="PKI05_11145"
     CDS             <1..1161
                     /locus_tag="PKI05_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52800.1"
                     /translation="IPYLNDSFYLEKALQPVTDESWALKPSILTAAALTSDPLSNYRI
                     IYLVNLPEIDRPMAQRLVEFLQSGGKLVWICGQDTQVASYNAANEAVNGQLLPAALDS
                     VMAASTEENRDSWQIAWIDVQHPALVGLNDPPSLHQSVLVYKFIKTHSPVGSGARVLA
                     RLDGAGDPLILERAVGQGTVVWVGTSVHVDWTNLPLRPFFVPFMTRLAFYLAGTEPER
                     LKVLAGQPIVIPYQGQIPPSVVEVVPPDGALLRLNLERGQTPEFRYVDTHAVGFYQFR
                     LLASVRPIQKLYAVNPDPDESDPEKISREQLEEAFSETTVLFADNPEDLAPTFALLRH
                     GRSLSELFLTMVLIGLVFETFISNWFSPKEQDQQPKLLSQYRPRQRLSRTVG"
     gene            complement(1234..>2491)
                     /locus_tag="PKI05_11150"
     CDS             complement(1234..>2491)
                     /locus_tag="PKI05_11150"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOA52801.1"
                     /translation="FWVDVFVSKDVPPGTYRGTLKVISQEGQVVAMPVNLEVWDFTLP
                     RVPSLVTALGSPAERMRAYYARQSKEGKESEQVNWATVEKQCALLAQEHRINAYPPSD
                     WLRLEREGEEFRLSDENVARLREWIDTYCVNAIQVPSPVGIVKDPQANNQALQAWARA
                     WDRAIESIDRPHVVYYIYLKDEPNDPEAYEFVRVWGKAIRATKTKVKVMVVEQTRTQN
                     PAWGDLYGAVDIWCSLFPLYDEPTARERQALGEIMWAYTALCQLAKTPWWHIDYPLLN
                     YRVPCWMSYRYDIRGLLYWGGMSHWSQVEDPWTDPRTYRPGRSNRPLIYNGEGTLVYP
                     AKPCGYDGIVPSLRLKALRDGIEDYDYFMILQRAGQGEKALKIVQQLAPSWFEWNPDP
                     QAYEAARRELAKAILSLPNEVRNPTW"
BASE COUNT          552 a          733 c          633 g          573 t
ORIGIN      
        1 atcccctatc tcaatgactc cttttatctc gaaaaggcgc ttcagcccgt cacggatgaa
       61 tcgtgggcat taaagccatc catcttgaca gccgcagcac tgacaagtga tcccctgtca
      121 aattatcgca tcatctacct ggtgaatttg ccggaaatcg acagaccgat ggcccagcga
      181 ttggtggagt tcctccagag cggtggcaag ctcgtttgga tctgcggtca ggacacacaa
      241 gtggcctcct acaatgccgc caacgaggcc gtcaatggcc agctcttacc cgcagcgctc
      301 gatagcgtta tggcggcaag cacggaagag aatcgagata gttggcagat cgcttggatc
      361 gatgttcagc atcctgctct ggtcggtttg aacgacccgc catccctcca tcaatcggtt
      421 ctcgtttaca aattcattaa gactcatagc cccgtgggaa gtggagcccg ggttctggca
      481 cgcctggacg gcgctggaga tccccttatt ctcgaaaggg cggtagggca ggggactgtt
      541 gtttgggtgg ggacgagcgt ccatgtcgat tggacgaacc ttcccttgag accctttttc
      601 gtccccttta tgacccgcct ggccttctac ctggcgggaa cggaaccgga acgattgaag
      661 gtcctggcag gacaaccgat cgtgatcccg taccagggtc agattccccc atctgttgtt
      721 gaggtcgttc cacccgacgg cgccctgtta cgactgaatt tggaacgcgg gcagacgcct
      781 gagtttcgct acgtggacac gcacgcggtc gggttttacc aatttcgact gctggcctcg
      841 gtaagaccta tccagaagct ctatgcggtc aacccagacc cggacgaaag tgatccagaa
      901 aagatttctc gggaacaact agaagaggct ttctccgaga cgacggtcct tttcgcagat
      961 aatcccgaag acctggcccc gacttttgcc ctcctccggc acgggcgaag cctgtccgaa
     1021 ttgtttttga cgatggtact tatcggcctc gttttcgaga cgtttatctc caactggttc
     1081 agccccaagg agcaggacca gcaacccaaa cttctctcgc aataccggcc ccgccagcga
     1141 ctcagccgaa ccgtgggcta aacggctacg acatcagcag gagatatcct gccgaaaaag
     1201 gtactggcgt tttactgaag aacgtctaag ttattaccaa gtcggatttc gaacctcgtt
     1261 cggcaacgag agaatcgctt tcgccaattc ccgccgggcc gcttcgtagg cttgtgggtc
     1321 gggattccat tcaaaccatg acggggcaag ctgctggaca atcttcaggg ccttttcgcc
     1381 ttggccagca cgctgcagga tcatgaaata gtcatagtct tcaatcccat cgcgcaaggc
     1441 ctttaaacgc aggctgggga caatcccgtc atagccacac ggcttcgcgg gataaaccaa
     1501 cgtcccctcg ccattgtaga taagtggccg attcgaacgt cccgggcgat aggtccgggg
     1561 gtcagtccac ggatcttcga cctgcgacca gtgactcatg ccaccccaat aaaggagccc
     1621 acgaatatcg tagcgataag acatccagca cggcacccgg taattgagga gcggataatc
     1681 aatgtgccac caaggcgttt tggcgagctg gcacagagcc gtataagccc acatgatttc
     1741 gcccagtgcc tgtcgttctc gggccgtggg ctcgtcatac agtggaaaga gcgagcacca
     1801 aatgtccacg gctccataaa ggtctcccca agccgggttt tgagtccgcg tttgctcaac
     1861 aaccattact ttgactttgg ttttcgtagc ccggatggcc tttccccaca cacgtacaaa
     1921 ctcgtacgct tccgggtcgt tcggctcatc tttcaggtag atatagtaca ccacatgcgg
     1981 tcgatcgatg gactcgatgg cacgatccca tgccctcgcc cacgcttgca acgcttgatt
     2041 gtttgcctgt gggtctttga cgattccgac cgggctcggg acctgaatgg cattgacgca
     2101 atacgtgtca atccattctc ggagccgtgc gacattctcg tccgacaagc gaaattcttc
     2161 tccctcacgt tcaagcctca accaatcact aggtgggtaa gcattgatcc gatgctcttg
     2221 ggccaagaga gcacactgtt tctctacggt ggcccaattc acctgttccg attcttttcc
     2281 ctccttggat tgtcttgcgt agtacgctct catccgttct gctggcgaac ccaatgccgt
     2341 cactagacta ggcaccctcg gcaacgtgaa atcccaaacc tccagattga ccggcatcgc
     2401 tacgacctgc ccttcttggg agattacttt caatgtgccg cgataggttc ccggtggtac
     2461 gtccttagaa acaaacacat caacccaaaa t
//
LOCUS       DAOPTR010000603         2489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_33360_length_2489_cov_3.912490, whole genome shotgun sequence.
ACCESSION   DAOPTR010000603 DAOPTR010000000
VERSION     DAOPTR010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33360_length_2489_cov_3.912490"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1497
                     /locus_tag="PKI05_11155"
     CDS             <1..1497
                     /locus_tag="PKI05_11155"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOA52802.1"
                     /translation="HPSPRRFASRVEEVEILRFATREQPADAQAWFQLGCVLANLGRI
                     EEAVPAWQKAVELDPHNSVALRNLGLEATTRNDLTTAERFYRQAIQARPDDQTLYRDL
                     AEILIASNRRSEAIALLEAMPISGVRRADVAVMLAQAYLDEDRYDDCLRLLENTPHFT
                     NWEGQDIVWRLFNRAHILRGRQRLDQGDARSALADFEAALTYPENLGVGRSNKPIEAP
                     AQYWRGRALVALGRYREARDAWVVGAELPAVPGEQDEYREKCRQALQELPPVPEDPIF
                     IHGPTYRCFKIERDLVLTGAMDDPLWQAAPVAVLNEPVEGRPGRYRTTARLLYNDRYL
                     YVGFQCEDELVWGTHTERDAPVYEEECVEVFICPTGQPRLYYEINVSPLNTVFDAFIL
                     NGRPVGGPRINFRGLKDYTCEGLVTRVFIDGKLGEPGARGWSAEYAIPFSSLVGCVPG
                     TPKPGTTWFLNLFRIDAPDPKKPEFYSWVPTTAIDFHRPWCFGMIRFD"
     gene            1561..2352
                     /locus_tag="PKI05_11160"
     CDS             1561..2352
                     /locus_tag="PKI05_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52803.1"
                     /translation="MKPTCPSCHQEIPVEDVDLDTGLGRCRFCNEIFEIPELVEHIQK
                     IQVKDTKEPKSPPVRPADTRIRLVRKEDKLLIYLPPWGLRGPTIALFLFSLFWLGFIA
                     FWTAGALGIFFQPGKAPRWENIIFSLFSIPFWLIGFGMLGGALSGMLSRRIVYLDSSW
                     LESRWECLFVKFRKQISREDVQMARVASPPVFRSTTNDQPNPTFGIEIIYQGGKISVP
                     CESEAEQDWLLAEVNHFLETVPYRPNYLTEGAADEAIKDLSVEGS"
BASE COUNT          498 a          672 c          732 g          587 t
ORIGIN      
        1 catccgtcgc cgcggcggtt tgcctctcgg gtggaagagg tcgagattct tcgttttgcc
       61 acgagggaac agcctgccga cgcccaggcg tggttccagc ttggttgtgt gctggccaat
      121 cttggacgga tcgaggaggc tgtgccagcc tggcagaagg ccgtggagct tgatccgcac
      181 aacagtgtgg cactgcggaa tttgggactc gaagcaacaa cgcggaatga cctaactact
      241 gccgaacggt tctatcgtca ggcgattcaa gctcgaccgg atgaccagac gctctatcgt
      301 gatctggcgg aaatcttaat cgcctcaaat cgccgcagtg aggccattgc cctcttggag
      361 gccatgccga tttccggcgt tcgtcgggcc gacgtcgcgg tgatgttggc ccaggcgtat
      421 ctggatgagg atcgttacga tgactgtctg cggctattgg aaaacacacc tcatttcacc
      481 aattgggagg ggcaggacat tgtctggcgg cttttcaacc gtgcccatat cttgcggggg
      541 cggcaacgac tcgatcaggg cgatgcccga tctgctctgg cggacttcga ggcggccttg
      601 acgtatcccg agaacctcgg agttgggcga agcaataaac ccattgaggc acctgcccaa
      661 tattggcgcg gtcgtgcgct tgtggcgcta ggtcggtatc gtgaagcccg cgatgcctgg
      721 gttgttggcg cggagcttcc cgcggtgccc ggcgagcaag atgaataccg cgagaaatgt
      781 cggcaggccc tgcaagaatt gccgcccgtg ccggaggacc caatctttat tcacggcccc
      841 acgtatcggt gttttaagat cgagcgtgac cttgtcctaa cgggggccat ggacgatccc
      901 ttatggcagg ccgcccctgt ggcggtgctg aacgaacccg tcgagggccg tcccggtcgc
      961 taccgcacga ccgcccgact cttatacaac gatcgctatc tgtatgtggg atttcaatgt
     1021 gaagatgaac ttgtttgggg aacgcatacc gaacgggatg cgcccgttta cgaggaagaa
     1081 tgcgttgaag tcttcatttg cccgaccgga caacctcgcc tttactacga aatcaatgtc
     1141 agcccgctca acaccgtgtt cgatgccttt attctcaacg gtcggccggt gggtggccca
     1201 cgcatcaatt tccgcggttt gaaggattac acgtgcgagg gccttgtgac gcgggtcttt
     1261 atcgatggca agctcggcga accaggggcg cggggatggt cggcggaata tgccattccg
     1321 ttctcgtcac tggtgggttg tgtgccggga acgcccaagc cggggacaac gtggttcttg
     1381 aatctcttcc gtatcgatgc ccctgatccc aagaaaccgg agttttattc gtgggtacca
     1441 acgacggcca tcgactttca ccgcccgtgg tgttttggta tgatccggtt tgattgatat
     1501 tgctaggcgg aagcggggca tgacgaagtt gcggactgtt gtttggtagg atcacgttgc
     1561 atgaagccca cctgtccgtc gtgtcaccaa gagatccccg tggaggacgt ggacctggac
     1621 acaggcttgg ggcgctgccg tttttgcaac gaaatcttcg agattccaga gcttgttgaa
     1681 catatccaga agattcaggt aaaagatacc aaagaaccca agtcaccgcc ggtgcgccct
     1741 gctgatacgc ggattcgcct ggtccgaaaa gaggataaac ttctcatcta tttgccgccc
     1801 tggggattgc ggggaccgac aattgccctg tttcttttca gcctattttg gctgggattt
     1861 atcgcctttt ggacggccgg tgccttgggc atcttttttc agcctggaaa agcacctcgt
     1921 tgggaaaaca ttattttttc gctcttttcg atccctttct ggctcatcgg ttttggcatg
     1981 ctgggtggag cactctcggg catgctttcg cggcggatcg tttacctgga tagctcgtgg
     2041 ttggaaagcc gctgggaatg cctgtttgtc aaattccgca aacaaattag tcgtgaagat
     2101 gtgcagatgg ctcgcgtggc cagcccgccg gtcttccgat cgacgacaaa tgaccaacct
     2161 aaccccacat tcggtatcga gatcatctat caaggaggca aaatcagcgt gccctgcgaa
     2221 agtgaggccg agcaggattg gctgcttgcc gaggttaacc attttctcga aacagtacct
     2281 taccgtccga actacctgac cgagggagcg gctgatgagg cgatcaagga cctttccgtg
     2341 gaagggtcat gatttctgct atcttaggcc ggccatgccc gcggccccgg tgaaatgaac
     2401 cgggcgacca ttttgacctg cccattgatc ccttcggatt gccccaagag aggtttgccc
     2461 catgaacaat gactttcccc aatcgcttg
//
LOCUS       DAOPTR010000604         2485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_33435_length_2485_cov_2.755967, whole genome shotgun sequence.
ACCESSION   DAOPTR010000604 DAOPTR010000000
VERSION     DAOPTR010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33435_length_2485_cov_2.755967"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1487
                     /locus_tag="PKI05_11165"
     CDS             <1..1487
                     /locus_tag="PKI05_11165"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA52804.1"
                     /translation="GAGVSAIRTFTRGLGVDAVLITAATSSNSPVELAAEIARDRARV
                     VMVGVTGMNIPRKPYYEKELTFIVSRSYGPGRYDLQYEEHGHDYPPGYVRWTEGRNLE
                     AFLDLVAAGAVRPEVLTTHRFPIDEAALAFELILKGKEPYLGVVLTYPTDTPAQPPGR
                     ITIRSSPPKPISKDTLGVSFIGAGSFAQSVLLPELVRLPGVQLRGIVSASGVTAKTVG
                     TKFGFAWCASQIDEILNDNETDIVFIVTRHSQHAPMVCRALEAGKAVFCEKPLAINFP
                     QLEQIAHTLEQFPGPLMVGFNRRFSPLANEMKDFLANRGPLQITYRVNAGTVPSDHWL
                     SDPSEGGRIIGEACHFVDLMSYLTGAPLVSATATPPDGADPRNETQAVFQFSDGSTGH
                     LLYTASGPPGWSKERIEVFAGRRAAVLEDFRKLELIDESGRRRRRRLWNADKGHRAML
                     AAWLAAVREGRPLISFQELFLTTAATFRVAGAQPHSESQKEDGP"
     gene            1484..2182
                     /locus_tag="PKI05_11170"
     CDS             1484..2182
                     /locus_tag="PKI05_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOA52805.1"
                     /translation="MSESQAKLPADVWVVVPAYNEAARLGNTLQDLLTVAQTVVVVDD
                     GSKDDTYDVASQYPVWLLRHVVNLGQGAALQTGITFALRQGAQYVATFDADGQHDARD
                     LLSMLEMLQSSGADYALGSRFLGRAEGIPWHRRLILRAAVLFTRLLSGAVVSDTHNGI
                     RLMTRRGAQRIHITLNRMEHASQIIDQIVASGLPFIEVPVTIRYTQASLAKGQKTSAA
                     VRMGLKLIVEKAFR"
     gene            2179..>2485
                     /locus_tag="PKI05_11175"
     CDS             2179..>2485
                     /locus_tag="PKI05_11175"
                     /inference="COORDINATES: protein motif:HMM:NF021575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HOA52806.1"
                     /translation="MNVFQLIFAPLCALLAAWNALRGWRRMTSRRAALFWTILWAGAA
                     LAIAVPDLAGLAARTLGIGRGADLVFYLAILAGLIACRYFYNRYRRLENVVTVLVREH
                     "
BASE COUNT          476 a          733 c          742 g          534 t
ORIGIN      
        1 caggtgccgg agtctcggcc attcgcacct tcacccgggg actgggagtt gatgccgttc
       61 tcattaccgc agccacatcg agtaacagcc cggttgagct cgctgccgag atcgcccgcg
      121 accgtgctcg agttgtcatg gtcggcgtca ccggaatgaa tatcccgcga aagccttact
      181 atgagaagga actaaccttt atcgtttcgc ggtcatacgg tccaggccgc tacgatctcc
      241 agtacgaaga acacggtcac gactacccac cgggttatgt ccgctggacg gaaggccgaa
      301 acttggaagc gtttctcgac ctggtggccg caggtgccgt tcggccagag gtcctcacca
      361 cgcaccgctt tcccatcgac gaggcagctc tggcattcga actcattctg aagggaaagg
      421 agccatacct aggggtcgtt ctcacctatc cgaccgacac gcccgcccaa cctccggggc
      481 gaattaccat ccgcagttcg ccgccgaaac cgatctcgaa ggacactctc ggggtttcct
      541 ttatcggggc gggaagtttc gcccaaagcg tccttttgcc cgagttggtg cgcctgccgg
      601 gtgttcagct ccgcggtatt gtcagcgcat cgggtgtgac ggccaagacg gtcgggacca
      661 aattcggctt tgcgtggtgc gcctcccaaa ttgacgagat cctcaacgat aacgagacgg
      721 atatcgtttt tatcgtgacg cgtcattcgc agcacgcgcc gatggtctgt cgcgccctag
      781 aggcgggaaa ggctgtgttt tgcgaaaaac cattggcaat caactttccc cagcttgagc
      841 aaatcgccca tacactggag caattccccg gtccgcttat ggtgggcttc aaccggcgat
      901 tctctccgtt ggccaacgaa atgaaggatt ttctggccaa tcgcggacca ttgcaaatta
      961 cctatcgggt aaatgccggc accgtcccga gcgaccattg gctgtctgac ccctccgagg
     1021 gggggcggat catcggcgag gcctgccact ttgtggacct gatgagctac ctgaccgggg
     1081 ccccgcttgt gtcggcaact gcaacccctc cagatggtgc cgatccgcgc aacgagaccc
     1141 aggccgtgtt tcaattttca gacgggtcaa cgggtcacct tttatacacc gcttcggggc
     1201 cccccggctg gagtaaggag cgaattgagg tctttgccgg aaggcgggcc gccgtcttgg
     1261 aggatttccg caagctagag ttaattgacg aatcaggccg acgtcggcgg cggcgactgt
     1321 ggaatgccga caaagggcac cgggcgatgc tcgcagcgtg gttagctgct gtgcgggaag
     1381 gacggcccct tatttcgttt caggagttat tcctcacaac ggcggccacg ttccgtgtgg
     1441 caggagcaca acctcacagt gaatcgcaaa aagaagacgg gccatgagtg agtcgcaagc
     1501 aaaacttccg gccgatgttt gggtagtggt gccggcgtat aacgaggcgg cccgcctggg
     1561 gaacactttg caagacctcc tcaccgtggc ccaaacggtt gtggtcgtcg atgacggttc
     1621 gaaggacgac acttacgacg ttgcgtcgca atacccggtg tggctcctcc ggcatgtggt
     1681 gaatttaggt cagggcgcgg ctttgcaaac ggggataacg ttcgctttgc gccaaggtgc
     1741 ccagtatgtg gccaccttcg atgccgacgg ccaacacgat gcccgcgatc tgctcagcat
     1801 gttggaaatg ttgcaatcca gcggggccga ttatgcctta ggttcgcgtt ttttgggccg
     1861 ggctgaaggc atcccctggc accgccggct gatattgcgg gcggccgtgc tctttacgcg
     1921 gcttctgtcc ggtgccgttg tcagcgatac gcacaacgga atccgactga tgacccgccg
     1981 cggcgcccag cgcattcaca ttacgcttaa ccgcatggaa cacgccagcc agattatcga
     2041 ccagattgtg gcgtcgggct tgccatttat tgaggtcccg gtcaccattc gttacacgca
     2101 agcatccctg gccaagggtc agaagacctc cgcggcggtt cgcatgggtc tcaagttaat
     2161 tgtggaaaag gccttccgat gaacgttttt caactcattt tcgctccgct ctgtgcgcta
     2221 ttggccgcgt ggaatgccct tcgcggttgg cggcggatga catcgcgccg ggcggccctc
     2281 ttttggacga tcctctgggc tggagcagcg ttagccatcg cggttccaga cctggcgggg
     2341 ctggcggctc gtacgctggg tatcggccgt ggcgccgacc tcgtgtttta cctggcaatc
     2401 ctcgccggcc tgatcgcctg tcgctacttc tacaaccgct acagaagact ggagaacgtg
     2461 gtaaccgtat tggtgcgaga gcatg
//
LOCUS       DAOPTR010000605         2483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_33471_length_2483_cov_3.324135, whole genome shotgun sequence.
ACCESSION   DAOPTR010000605 DAOPTR010000000
VERSION     DAOPTR010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33471_length_2483_cov_3.324135"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="PKI05_11180"
     CDS             complement(<1..1013)
                     /locus_tag="PKI05_11180"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA52807.1"
                     /translation="MFAGPIFTRELAICPRRKQFFIARTAYGAILLLLLCTAWLVVTG
                     TQYIRDVGDSARFGAAWFQIAAPLQVILAIFTSALVAAASICQEKDKRTFDLLLVTDL
                     RNTELVLGKLLSSLVTLFAAFVGGIPVYFFAGLLGGISYGQILRVTLVTVVALLGGGS
                     LGALIAFWREKTVQAITLTAMGVVFWLGFGELAAWIGRSEILWGLTGQTLAVAISPWR
                     AMLEAAKPLLVETPSLGVLRYDLSLFFVVNGAAVILMNAIAIAGVRHWNVVRDPHRII
                     PEELPDEQGEDLKEDSASTSGAGKGTTTEADREQRRSRLARLHAAPGRAREVWDNPVL
                     WRE"
     gene            complement(1148..2401)
                     /locus_tag="PKI05_11185"
     CDS             complement(1148..2401)
                     /locus_tag="PKI05_11185"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HOA52808.1"
                     /translation="MLNRRKFLAVSGGALSAGVASIGTISLATTEVAGDLGGDETILV
                     ALQMAGGDDGLNTVVPYRDDNYRRLRPTLALKESDVIRLQDDLGFHPALKPLMPLWEE
                     GHLAVVQGVGYPQASDDHGRAMEIWQTARPEDPLADTGWLGRAADELFEKGNTARAAA
                     PAVFLGQIPIPLTVVGRHQPAVRCQQPAELFSLHGGAASLPHYRQLIHEATDGRQPAA
                     HGLLAETVEVQRQTKEHLSRLEKVLATSDEEKQFPQTNFGRQLACLTRLVRADLGIRI
                     FCLDLGGQGPGTFDTHALQAANHGELLAELAQGLALLADVLKNDGLFDRVVIYTYSEF
                     GRTIRENGRKGTDHGAAAPVFVLGGKVKGGLVGKHPPLDVPEKGGVPHHTDFRSLYAT
                     FLDRWLHVPSERVLGRSLGHLPLFA"
BASE COUNT          544 a          783 c          708 g          448 t
ORIGIN      
        1 tgttctcgcc agagtaccgg attgtcccag acttcccggg cccgaccagg ggcagcgtgc
       61 agtcgggcca ggcgagacct gcgctgctcg cgatccgcct ccgtggtggt cccttttcct
      121 gctccgctgg ttgaagccga gtcttctttg aggtcctcgc cttgctcgtc gggcaactct
      181 tccgggatga tccgatgggg atcgcgcacc acgttccagt gtcgcacgcc cgcaatggca
      241 atggcgttca tcaggatgac agcggcccca ttcacgacga agaagagtga cagatcgtac
      301 cgcaagacgc ccagcgaagg cgtctccacg agcagtggct tagccgcctc gagcatggcc
      361 cgccagggac tgatcgccac agcgagggtt tggcccgtca atccccaaag gatttcagat
      421 cgcccaatcc acgcggccaa ttcaccgaac cctaaccaga agacgacacc catcgcggtc
      481 agggtgatcg cttggacggt tttctcccgc cagaaggcga tcagggcccc gaggcttcct
      541 ccgccaagca gggcaaccac ggtgacgagc gtcactcgca aaatctgccc gtaagagatt
      601 ccccccaaga gcccagcaaa gaaatagacg ggaatacccc ccacaaaggc ggcaaacagg
      661 gtcaccaggc tactgaggag cttcccgagc acgagctccg tattccgtag atcggtcacc
      721 aggagcagat cgaatgtccg tttgtctttc tcctgacaaa tactggccgc ggccactagg
      781 gcggaagtaa aaatcgccag aatcacttgg agcggggccg caatctgaaa ccatgccgcg
      841 ccaaatcggg cagaatcccc cacatcgcga atgtattggg tccctgtgac gacgagccag
      901 gccgtgcaca aaaggagcaa caaaatcgca ccataagccg tccgcgcgat gaaaaattgc
      961 ttgcggcgcg gacaaatggc caactcacgg gtaaaaatgg gaccggcaaa caaggggctc
     1021 tccgttaagg ttctctcaca gcaaatggac gtttcttccc atcatagatt gccgacgccg
     1081 aatctgaaag agattggcct ttttgccggc ccggccacga cggcgcgacg gcctccaccg
     1141 cccaacatca cgcgaaaagg ggaaggtgtc ccaaacttcg ccccaggaca cgttcactgg
     1201 gcacatgaag ccaccggtcg agaaacgtcg cataaaggct gcggaaatcg gtgtgatgtg
     1261 gcacgccccc tttttcgggt acatctagcg gcgggtgctt tcccaccaaa ccgcctttca
     1321 ccttgccacc tagaacgaaa acaggggcgg cggcaccgtg atcggtcccc tttcggccat
     1381 tctcgcggat ggttcgtccg aactccgagt aggtataaat caccacacga tcaaagaggc
     1441 catcgttttt gaggacgtcg gcaagcaagg ccaacccctg ggcgagttcc gccaacagtt
     1501 ccccatgatt ggctgcctga agggcgtggg tgtcgaaagt ccccggcccc tgcccgccca
     1561 agtccaggca aaagatgcga atgcctaagt ccgcccgcac caatcgggtc agacaagcaa
     1621 gttgtcggcc gaaattcgtt tgtggaaatt gcttctcctc atcggaagtg gccaagacct
     1681 tctcaagccg cgagagatgt tctttggtct gccgctgaac ctcaaccgtt tcggccagaa
     1741 ggccatgtgc cgcgggctgc cgaccgtcgg tggcctcgtg gattaactgc cggtaatggg
     1801 gcagcgaagc ggcgcccccg tggagggaaa acagctccgc cggttgctgg cagcggaccg
     1861 caggctggtg tcttcccacg acggtcaacg ggattggaat ctggccgaga aacacggcag
     1921 gggctgccgc gcgggcggtg ttacccttct caaaaagctc atccgcggca cgaccgagcc
     1981 agccggtgtc ggcaagcggg tcttcgggtc gggcggtctg ccaaatctcc attgcccggc
     2041 cgtgatcgtc cgaagcctgg ggataaccaa ccccctggac caccgcgaga tggccctcct
     2101 cccatagcgg catcaaaggc ttgagggccg gatgaaaccc caaatcgtcc tgaaggcgaa
     2161 tcacatccga ttccttcagg gccaatgttg gccgcagacg gcggtagtta tcatcccgat
     2221 agggcaccac cgtgttgaga ccatcatccc ccccagccat ctgtagggcc accagaatgg
     2281 tctcgtcccc gccgaggtct ccagcaacct cggttgtcgc cagagaaatt gttccaatgg
     2341 aagcgacgcc ggcacttaag gctccgccgg aaacggccag gaacttgcga cggttcaaca
     2401 tgggcacttt ctccttttgg gacggtcgtg attgggttgt tacgccgtag cacctagtcc
     2461 ttcctcgcga tcaagaccac tgg
//
LOCUS       DAOPTR010000606         2480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_33521_length_2480_cov_4.193814, whole genome shotgun sequence.
ACCESSION   DAOPTR010000606 DAOPTR010000000
VERSION     DAOPTR010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33521_length_2480_cov_4.193814"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..1053
                     /locus_tag="PKI05_11190"
     CDS             253..1053
                     /locus_tag="PKI05_11190"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOA52809.1"
                     /translation="MMKATMSNVFAELVLVILVAFVTEKVSAAENGPSTLGKNDPVLE
                     RIDFIYSEMVPLSWTPPSDRWRNLERSIATLKHGGTLRVVMLGDSIVNDTSRSAWEKL
                     VARLYPGAHIEKITSVRGSTGCWYYKDPNQLEEYVLKYKPDLLMIGGISHRDDIESVR
                     EVIRGVRANLEPDPEILLMTPCFGHKDPRMDPEWTFEVPKDGNDFRSRLKRLAEEENT
                     AFLDMTGPWGQYIRTCGEDIHWFKRDIVHANARGEAILGRILEKFFAP"
     gene            complement(1071..2081)
                     /locus_tag="PKI05_11195"
     CDS             complement(1071..2081)
                     /locus_tag="PKI05_11195"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOA52810.1"
                     /translation="MTRQTRGYLSILILVCFVVGCRSYPWSPESVAQGPLEGQSRSTL
                     AYDDQSKTAQAAPKKGSKAQAEEEAEYARKLAQGRSLERLGKLDEARKTYEGLIARFP
                     TRYEAYHRLAVVSDRQMRYTEAEALYAQAIRLNPTNPDLFNDLGYCYLLQGKPEKAER
                     ALLKAVGMAPASERYRNNLGLAYGLQGRYEEAMEQFRRANGEADAWYNLAYVQWMRND
                     IHLSKQSLENALAYNPSHTAAKESLAQLASGQSFFAESTGERRSGYRLAPDTESELIA
                     ANGAPSSSDQSVTQTTFEMVPGTLRLGLKDRPSTQTQLDVARSSWAKRLQQETAPLEL
                     AE"
BASE COUNT          549 a          628 c          719 g          584 t
ORIGIN      
        1 accccaaccc tctcccgaag ggagaggggg ctttttggga tagattcata ggtgtttcaa
       61 ataggaattt caagaaccca tagtacacag gtaatccgac tggcggcaga gatgtgggct
      121 tgtgcaaccg gacttgcact ggagacgcat gtgctcagaa tgtcgcgcgt gtttgggtgt
      181 cctggcgtgt tgcagaggaa aagtggtagt cttttttttc ctggtgagaa aggtggttgc
      241 gaggtgtccg atatgatgaa agccacaatg agtaacgtct ttgctgaatt ggtactcgta
      301 atattggtgg cgtttgtgac ggaaaaggtt agtgccgccg aaaatggtcc aagcacgctc
      361 ggaaagaacg atcccgtgct agagcgaatt gatttcattt atagcgagat ggttcccctg
      421 tcgtggacgc ctccttcaga ccggtggcgt aacttggaac gttcgattgc gacgctaaaa
      481 cacggtggca cccttcgcgt ggtcatgctt ggcgatagta tcgtaaatga tacgagccgt
      541 tcggcatggg aaaagctcgt ggcacgcctt tatcctgggg cccacatcga gaaaatcacg
      601 tcagtacgtg gcagcacagg gtgttggtat tacaaggacc ctaaccagct cgaagagtac
      661 gtgctcaagt ataagcccga cctgctcatg atcggaggta ttagtcaccg cgacgatatc
      721 gagtctgttc gggaagtgat tcgcggtgtc cgggcgaact tggaacctga ccccgagatt
      781 cttctcatga ccccatgttt cggccacaag gatccgcgga tggatccaga gtggacattc
      841 gaggtaccca aggatggcaa cgactttcgg tctcgactaa aaagacttgc cgaggaggaa
      901 aacacggcgt tcttggacat gactggaccc tggggccagt acattcgtac ctgcggggag
      961 gatattcatt ggttcaagcg cgacattgtc cacgcaaacg ccaggggtga ggcgattttg
     1021 ggcagaatct tggaaaagtt tttcgcaccc taacagtgcg gcctcaacat tcactcggcc
     1081 aactcaagag gggctgtttc ttgttgaagc cgtttggccc aagaggatcg cgccacatca
     1141 agttgcgttt gggtgctggg acggtccttg agaccgaggc ggagcgtgcc cgggaccatt
     1201 tcaaacgttg tctgtgtcac ggactgatca gaagaggacg gcgcaccatt ggccgcgatt
     1261 agctcagact ccgtatctgg ggccagccga tagccactgc gacgctcccc ggtgctttct
     1321 gcgaaaaagg attggccaga cgcaagttgg gcgagacttt ctttggccgc ggtgtgtgac
     1381 ggattgtaag ccagcgcatt ctccaaggat tgcttggata gatggatgtc gttacgcatc
     1441 cattgaacgt acgccaggtt gtaccaggcg tccgcctctc cattggcacg gcgaaattgc
     1501 tccatggcct cttcgtagcg gccctggagt ccatacgcca atcccaggtt attacggtac
     1561 cgttccgagg caggagccat tccgacggct ttcaagaggg ccctctccgc cttctcaggt
     1621 tttccctgga gcaagtaaca atagccaagg tcgttgaata ggtctggatt ggtcggattg
     1681 agacggatgg cttgggcgta aagtgcctcg gcctccgtat agcgcatttg gcgatcgctc
     1741 acaaccgcta gccggtggta ggcctcgtat cttgtcggga agcgggcgat taatccttca
     1801 taggtcttgc gtgcttcatc cagcttgcct agcctttcca aagaacgccc ctgcgccaat
     1861 ttgcgggcat actcggcttc ttcttccgct tgcgctttcg accccttttt aggggccgct
     1921 tgtgccgttt tcgactggtc gtcgtaagca agagttgatc ttgattggcc ttctagaggg
     1981 ccttgagcca ccgactcagg actccagggg tacgaacgac atcccacaac gaagcagacc
     2041 aggatcagaa tagaaagata tccccgagtt tggcgagtca caccgaggtt cctcatgaaa
     2101 cggtagggcg ttgatagaag ttccgcacac agaggccccg taaatcgcac cacaaagtac
     2161 tacctggccg gtagcgcgtt atcgaagaca gctaaagacg ccgggcaagg tcgccgagaa
     2221 tgtaccggtc gtattgatgg tatcgaatga tcgtcttgga taagttcaat caaaacgagg
     2281 gatttccata cgcaacccgc ccgatgtcat ttttgctcta catcagacga tggttttttc
     2341 cggggattcg acacctgtgg cacgggcggc ttacggttgg ccgcctcaat tctggcgcgc
     2401 gcggcatggc ctagatattg gcgcaacact tgatcggacc gaaggaggct ttcgcgtaat
     2461 gcgtccgctg actgggaatc
//
LOCUS       DAOPTR010000607         2474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_33645_length_2474_cov_2.381149, whole genome shotgun sequence.
ACCESSION   DAOPTR010000607 DAOPTR010000000
VERSION     DAOPTR010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33645_length_2474_cov_2.381149"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            566..1825
                     /locus_tag="PKI05_11200"
     CDS             566..1825
                     /locus_tag="PKI05_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA52811.1"
                     /translation="MKRNQFSRRFFLQSLAATTVSVPWLVQGRALGLEDAAPASERIT
                     LGHIGVGGRGRDLLRVFMTCPGAQPLAVADAYRDRRDAMAEMIKGKAYGDFRELLARD
                     DIDAVVIATPDHWHVPIAVMAARAGKHAYVEKPLGLTVQQDLICRKVFQETNRVFQYG
                     TQQRSMAHCRFGCELVRSGKIGELKRIEVVAPDGGAGGSTEPAPVPPELDYEMWIGPA
                     PMKPYTVSRCVTPGTYWIYDYSIGFLAGWGAHPLDIMIWGCDADLAGPMTFEGTGEIP
                     KEGLYDTVIHWDVTVQMGNGIVMTFKPGSDSTKFIGTEGWVDVRRSGIDAEPKSLLKL
                     ELGPDDVHLTKSPRHDQNFIEAIKAGGKAIAPIEEAVRSDIISHMCDIAIRTGRKITW
                     DPQKEEIVGDAEASKMLSRPMRAPWTL"
     gene            complement(2049..>2474)
                     /locus_tag="PKI05_11205"
     CDS             complement(2049..>2474)
                     /locus_tag="PKI05_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52812.1"
                     /translation="RCGNVEKPCIFMHPPYQGGVGYVAALLEPVQLPPEPAAALRCAV
                     GKGDGSDPGDGILYRVYVLDAQGQTTLLGEKVQREHAWADWEVDLGPWRGQQIVLKLV
                     ADVGNNNNSVGDWACWADLRIESREPQWVYTVLPVNPGR"
BASE COUNT          491 a          685 c          766 g          532 t
ORIGIN      
        1 gccggactga cgaaaatcaa ataaatagga tcaaagataa acatatttgc ctcaaaaacc
       61 tccctcggct gtcaatcctg ttcggaaatt cgacacacgt gtgtcttagc ctgtccctcg
      121 gggaaagccc ctttttactg tattctatgt tttcctgcgc ggttggccag tccgatttga
      181 ggtcgcacac gtcgcggttt acttccacgg ttggacaatc tgtagtagcg aggacgggct
      241 ctgccgctgt cggtagctgg gaggcgccgt ttgtcgcaat atgcggaggg acgcgggtcg
      301 tcgtgtccgc cgtggtgcca atggatgatc catggtcgtc gccgactacg gcagcgcgtc
      361 gccttccatt acccccatct tacgagcaag agcgtggtgg cttgcacggg caaatggggc
      421 ggtctaccat aaggtagcct tcctgaaggc ctccagatgt ccagtttgcc gaagggtctg
      481 tgaagcttcg gcaacatctt gggcgtaaag agcgccaccg gtcaagagcg tgtggattta
      541 cttgcacaat cgacgaggta cggccatgaa acgaaaccag ttctcgcggc ggtttttcct
      601 tcagtcgttg gcggcaacaa cggtcagtgt gccttggctc gttcagggac gtgcccttgg
      661 gttggaggac gcggctccgg ccagtgagcg gatcacgctg ggacatatcg gggtgggtgg
      721 acgtggtcgc gaccttttgc gggtgtttat gacctgccct ggcgcacaac ccctggccgt
      781 ggccgacgcc taccgtgatc ggagggatgc catggctgaa atgatcaagg gcaaggccta
      841 cggagatttc agggagcttc ttgcccgcga tgatatcgat gccgtggtca ttgccacgcc
      901 cgaccactgg catgtgccga ttgccgtgat ggctgcccgc gcgggaaaac acgcctatgt
      961 cgaaaagccg ctcggtctca ccgtgcagca ggatttgatc tgtcggaagg tattccagga
     1021 aacgaatcgc gtctttcagt acgggacgca gcaacggagc atggcccact gtcggtttgg
     1081 gtgtgagctt gtccgtagcg gcaagatcgg cgagctgaag cggatcgagg tcgtcgcacc
     1141 agatggcggc gctggaggtt ccaccgagcc ggcccctgtc ccgccggagt tggactacga
     1201 aatgtggatc ggtccggccc cgatgaaacc ctacaccgtc agccggtgtg tcacgccggg
     1261 gacatactgg atttacgatt actcgatcgg attcctggca ggttgggggg cccatccttt
     1321 ggacatcatg atctggggat gtgacgccga tttggcgggg cccatgacct ttgagggaac
     1381 aggtgagata cccaaggaag gtctctacga tacggttatc cactgggatg ttacggtgca
     1441 aatgggaaac ggcatcgtca tgaccttcaa gcccggtagc gactcgacga agttcatcgg
     1501 cacggaaggc tgggtggatg tccggcgatc gggaatcgat gcggaaccca agtcgcttct
     1561 caagttggaa cttggtcccg acgatgtcca cctgacgaag agtccccggc acgatcagaa
     1621 cttcatcgag gccatcaagg cgggcggtaa ggcgatcgct cccattgagg aggccgttcg
     1681 ttcagacatc attagtcaca tgtgcgatat tgctatccgc actggccgaa agatcacctg
     1741 ggacccgcag aaggaagaga ttgttggcga tgccgaggca tcgaagatgc tgtcccgtcc
     1801 gatgcgggca ccgtggacac tctaaatgaa acctacgtgt tagttgcgcc ggaactggga
     1861 gggacgcggt cccccgcgtc ccttgtcttt ttggagggac tcactccgtg gcgtgcgccc
     1921 tggttcgccg gcggaaggtt tggattatct tcggtcacga cacagcgcgg cgctgtatca
     1981 tcgccacccg gaggcaacgc ggtcaccacc gtccgggcgt gtgtctgcgg ctcgtgttag
     2041 ctgaacggtc aacggccagg gttaacgggc aagacggtgt aaacccattg tggttcacgg
     2101 ctttcgatcc gtaggtctgc ccagcaggcc caatcgccca cagagttgtt attgtttccc
     2161 acatcggcca caagcttcaa tacaatctgt tgacccctcc agggaccgag atcgacttcc
     2221 caatctgccc aggcgtgctc gcgttggact ttttcgccga gcagggtggt ctgcccttgg
     2281 gcatcaagga cgtaaactcg atagaggatt ccatctcccg gatcactgcc atcgcctttt
     2341 ccgacagcgc agcggagggc agcagcgggt tctgggggca gttggaccgg ttccagaagg
     2401 gccgccacgt accccacacc gccttgatac ggcgggtgca tgaagatgca cggtttttcc
     2461 acattgccac agcg
//
LOCUS       DAOPTR010000608         2472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_33683_length_2472_cov_3.117087, whole genome shotgun sequence.
ACCESSION   DAOPTR010000608 DAOPTR010000000
VERSION     DAOPTR010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33683_length_2472_cov_3.117087"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PKI05_11210"
     CDS             complement(<1..460)
                     /locus_tag="PKI05_11210"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOA52813.1"
                     /translation="MSDDSPENRHVTRRGIPTAADWDLAYREGLPPWEMGCPSPELVA
                     FLDKGLLQKASVLELGCGTGADAVFMAQRRLDVTAVDFAPTAIERARTRAQRAGVNVC
                     FVLDDVFHFAYRTQTFDFIYDCAFYQYIRQWELSRLLDLLWRVTKPGSYYL"
     gene            1079..2200
                     /gene="pheA"
                     /locus_tag="PKI05_11215"
     CDS             1079..2200
                     /gene="pheA"
                     /locus_tag="PKI05_11215"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOA52814.1"
                     /translation="MPKGARTAKAFRLPAKPTVAKLQQALADLDQRLLELCNLRAEMS
                     VQLSQLRREEGQPDFQLSDEDRQLAAVVEQNKGPLPPAAIKAVFRELISACRAMIKPL
                     RVAYLGPQYSYSHLAAIHRFGKQVDYVAVGSIAAVFEEVTRGHCQFGLVPLENSTDGR
                     IADTLDMFIRTPIRICGQVELEIHHSLLSKSSRSEIREVYSKPQALSQCRNWLAKHLP
                     GARLIEVTSTSTAAQLAAEKPGAAAIASIQAGIHYGLNTLAENIEDNPGNMTRFAVIG
                     NVICPRTGHDKTALMFQVEHRPGALAEAMNIFKRNRLNLTWIESFPVPGCRGAYYFFA
                     ELEGHESEPRVRRALASLSKKTQRLELLGSYPIPTPVST"
     gene            2322..>2472
                     /locus_tag="PKI05_11220"
     CDS             2322..>2472
                     /locus_tag="PKI05_11220"
                     /inference="COORDINATES: protein motif:HMM:NF024106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HOA52815.1"
                     /translation="MGRGPLKSETQAKESAPHLLRVISGGQTGVDRAALDAAIQLNIP
                     HGGWCP"
BASE COUNT          575 a          698 c          647 g          552 t
ORIGIN      
        1 agagatagta agagccgggc tttgtcacac gccagaggag gtcaagcagc ctcgacaact
       61 cccattggcg tatgtactga tagaacgcgc aatcatagat gaaatcaaag gtttgggtgc
      121 ggtatgcgaa atgaaagaca tcgtcgagga caaagcaaac attgacgccc gcccgttggg
      181 cccttgttcg ggcacgttca atcgccgtcg gagcgaaatc aacggccgtc acatcgaggc
      241 gccgttgggc catgaagaca gcgtcggcgc ccgtcccgca acccagctcc agcacacttg
      301 ctttctgtaa tagcccttta tccaggaaag caaccaactc tggggacgga caccccattt
      361 cccatggcgg cagcccttcg cggtaggcca aatcccagtc ggccgccgtt ggtattcccc
      421 ggcgagtcac gtgacgattt tcagggctat catcgctcat tgttttgctc gcccgtgttg
      481 actccgagtg atgcagaatt ccaccgacaa tccgatattt accgtcgatg cgaagcaatc
      541 gcccctaaga taccacaaac aaaatctggc ttctatgcca agatgttact tacgtaccca
      601 gcgccgtgtc atgttcacag ccaagatgcc ccttgggtcc cgttatatcg ttacggcctt
      661 taccctataa atggattggc taatagactc cttcaaactt cagcgagcta aaaaatggga
      721 aatgcaacca atttcccacg cttgcccttt gtctccgatg gggagtcagg acctcttcag
      781 gtcccgacca tcccagtgtc ggcgaagaac cattacagaa tcccactcgg cgacaaatcg
      841 atcccctccg aacggtgcag tagctatact cctcgtgggt ataaaccaac ctttccccga
      901 tcagctaccg gtttccccat atcgcgttct atccgatata acccctgatg ggggataaat
      961 taggccattg tgttcttttc ccagcgagca ccaattgaca acgccgtgcg gcgtaaatac
     1021 aagggtgaga tagggcttag gacccaattt cctagacctt ttcaacaggg ttttaggtat
     1081 gcccaaaggt gcaagaacgg cgaaggcgtt tcgcttaccg gccaaaccga ccgtggccaa
     1141 attgcaacaa gcgctggccg acctagacca gcgtcttctg gagctgtgca atctccgtgc
     1201 cgaaatgagt gtccaacttt cacagcttcg ccgggaagaa ggccaacctg attttcagct
     1261 ttccgatgaa gacaggcaac tggccgcggt ggtcgaacag aacaagggcc ccttaccgcc
     1321 cgctgccatc aaagctgtct ttcgggagct tattagtgcg tgtcgggcca tgattaagcc
     1381 gttgcgcgtg gcatatttgg gcccccaata cagttatagc catttggccg ctatccaccg
     1441 gtttggtaag caagtggact acgtcgctgt ggggagcatc gccgccgttt tcgaagaggt
     1501 cacgcgtggg cattgccagt ttgggctggt tcccttggaa aactccaccg atggtcgcat
     1561 tgctgatacg ctggacatgt ttatccgcac acccatccgc atttgcggcc aagttgagct
     1621 ggaaatccat cactccttgc tttcgaagtc ctcgaggtcc gaaattcgag aggtctacag
     1681 caaaccgcag gcgctatctc aatgtcgcaa ttggttggca aagcacctgc caggggcacg
     1741 gctgatcgaa gtcactagca cctccaccgc ggcccaactc gccgccgaga aaccgggggc
     1801 cgcagcgatt gccagtatcc aggcagggat acactatggg ctaaacacac tggcagaaaa
     1861 cattgaggac aatcccggca atatgacgcg ttttgccgtg atcggtaacg taatttgccc
     1921 gcggacgggt cacgacaaga cggccctcat gtttcaggtg gaacaccgac ccggggcatt
     1981 agccgaggcc atgaatatct tcaagcggaa tcgcttgaat ctcacatgga ttgagtcgtt
     2041 tcctgtgccc ggttgtcgcg gggcgtacta ctttttcgcg gagctggagg gacatgagtc
     2101 tgaaccgcgg gttcgtcggg cactggcaag tctaagcaag aagacccaac ggcttgagtt
     2161 actcggctct tacccaattc ccacaccggt tagcacctga ttgatgcaac ttgcggtcct
     2221 tcttgacaac aagagtctta tgcgagttga cggctccgct agtagggtac ctcgttggtt
     2281 tcgtcagtct gtttgggcca ttgggaaacc cggcacacct tatgggacgt ggtccattga
     2341 aaagtgaaac acaggcgaaa gaatcagcac cacacctttt gcgcgtcatt tctggtggac
     2401 agacgggagt cgatcgagca gcgctcgatg cggctatcca gctcaatatt ccgcatggtg
     2461 ggtggtgccc ct
//
LOCUS       DAOPTR010000609         2467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_33783_length_2467_cov_4.193615, whole genome shotgun sequence.
ACCESSION   DAOPTR010000609 DAOPTR010000000
VERSION     DAOPTR010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33783_length_2467_cov_4.193615"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PKI05_11225"
     CDS             <1..326
                     /locus_tag="PKI05_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52816.1"
                     /translation="TSTTSCCTFAMAAATTTGQAEGQTGRGRRKKGEAETPVEPVVTS
                     QQAVQPAAKEQEAATVSVPPGEKGRRGRPPASGEAASERKSRRGRPPKGVSDSEKKRG
                     RKKAT"
     gene            1017..1634
                     /locus_tag="PKI05_11230"
     CDS             1017..1634
                     /locus_tag="PKI05_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOA52817.1"
                     /translation="MAKKRNSQSPLASKTVFTTGEAAKICKVSQQTIIRCFDSGQLKG
                     FRVPGSRFRRIPRDQLIAFMRENGIPIDNLENTKHKILIVDDDQELVELIRDVLERDG
                     RFEVQSVNNGFGAGMMVKEYHPDLIVLDIMLPDINGKDVCKVVRSDPNLSEIRILCIS
                     GMVEEDRIEELKAAGANDFLHKPFEIEDLVERICQLLDIPSRATT"
     gene            1739..>2467
                     /locus_tag="PKI05_11235"
     CDS             1739..>2467
                     /locus_tag="PKI05_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52818.1"
                     /translation="MQWAVCARHEQCFLPVSDQTVLELGHVVLLLGQDLPQGAHAGKT
                     PADNSSLIRKILAIFSRDPLALATVLGSIPPASEASRGGDGPELALGRAILQGFWASL
                     GDDRHSPPLRLVEPRAAEKFSIDKVRILQAVQKIFLIQQLTRNWAELHQPQAVALIPF
                     AAVVIVPRFFLGNCSVNFVAELRRVLNPKDHKTLDVALEVVETCHGILGQLGEGAKLP
                     QLDSQLLAPLQRTQACYPGEVKEAT"
BASE COUNT          570 a          655 c          749 g          493 t
ORIGIN      
        1 ctacctctac caccagctgc tgcactttcg cgatggccgc cgcaacaacg acgggacagg
       61 ccgaaggtca aactgggcga ggccggcgaa agaaaggcga ggccgagacc ccggtcgaac
      121 cggtggtcac gtctcaacaa gccgtgcagc ccgcagcaaa agagcaggaa gccgctaccg
      181 tctctgttcc acccggggaa aaagggcggc gggggcgccc acccgcctcc ggtgaggcgg
      241 catcggagcg gaaatcccgg cgcgggcgtc cgccgaaagg ggtatccgac tccgagaaaa
      301 aacggggccg taaaaaggca acataatcaa aaaagcgctt gtctctgtta tatgagttcc
      361 gcgccctagg caggcatcgc ccaaaatggg gaagaatagg ggtaataggt cgtctcgtgg
      421 cggccgattc gccatgaggg ccgtgatgtt gccgccccat cggaaagatt cggcgactgt
      481 ctcataaggt gggtatttct gggggagatc atgagatagg taaaagtcga aggctagatt
      541 ccccacggtt ccgggccggg actgattaga cccacttggc tcagccgaga aaagctgacc
      601 cctccgcgca ttcgcggggc tagctgccgg taccgccacg agaggtcggg gctagccgct
      661 ttccatcaac gccaattacc gcaaattgcg cggcttttca tctgtgattc ggtaaaaagt
      721 tcgcatgggg aagtcttggg gacgtaacac ggtgtgggca agagagctaa agatgcaaaa
      781 gtcgtattga cagggatttt ggtgaaagta taatgatccg cggttggcaa attcggtaaa
      841 gtcggcagaa ccggatgagc ctgctgcttg taggcagcta cgggaggtgc aaggtttgcg
      901 ggaaaccgtg aacctgcggc ttctttcccg gatggagtaa cacggaggac cagagacact
      961 cagccaactg ccgaccgatg ggctggggtc tcctttggaa gattccggcg agaaccgtgg
     1021 ccaagaaaag gaacagtcag tcaccactcg caagtaagac cgtcttcaca actggtgaag
     1081 cggccaaaat ctgtaaggtc agtcagcaga cgattattcg ctgctttgac tcgggccaac
     1141 tgaagggatt tcgcgttccg ggaagccgat ttcgtcgtat cccccgggat caattgatcg
     1201 ctttcatgcg agaaaatggt atccccatcg acaacctgga aaacacgaaa cacaagattc
     1261 taatcgtgga cgacgatcaa gaactcgtcg agctgatccg ggacgtcctg gagcgggatg
     1321 gtcggtttga agtgcagagc gtcaataacg gatttggcgc cggtatgatg gtgaaggaat
     1381 accatcccga cctgattgtc cttgatatca tgctgccgga tatcaacgga aaggacgtct
     1441 gcaaagtcgt ccgctcggat cccaatctga gcgaaatccg gattttgtgc atctccggga
     1501 tggtggaaga agaccgcatc gaggaactga aggcggcggg ggccaacgat ttcctccata
     1561 agccgtttga aatcgaagac ctcgtggaac gcatttgtca gctcctggat attccgtccc
     1621 gagcgacgac ctgattcatt caccagtgcg agacggctcg cttgcaggaa acaagaatcc
     1681 gcgggagtgg tctgcctttg accggtgata gaggcagttc cagcaggagg ggcttgcaat
     1741 gcagtgggcc gtctgtgcgc gacatgagca atgcttcctc cccgtctctg accaaacagt
     1801 gttggagttg gggcacgttg tcttgctgct cgggcaggac ctccctcaag gtgcgcacgc
     1861 cggcaaaaca cccgcggaca actcctcgtt gatccggaag attctggcca tcttctcgcg
     1921 ggatccgttg gcccttgcca cggtgcttgg gtcaattcca ccagccagcg aggcaagccg
     1981 tggtggcgac gggcccgagt tggccttagg ccgagcgatt ttgcaggggt tttgggcctc
     2041 tcttggggat gacaggcact ccccgcccct gaggttggtc gagccgcgag cagcagagaa
     2101 attttcaatt gataaggtcc gaattcttca agccgttcag aaaatcttcc tgatccagca
     2161 gttgacccga aactgggcgg agctccacca gccccaagcc gtggcactca ttccctttgc
     2221 agcggtggtc atcgtcccga ggtttttctt ggggaactgc tctgtgaatt tcgtggcgga
     2281 actgcggaga gtcctgaacc caaaggatca caagaccctt gacgttgccc ttgaggttgt
     2341 ggagacctgt cacgggatac ttgggcagct cggcgaaggt gcgaagttgc cgcaattgga
     2401 ttcgcaacta ctcgccccgc tccaaagaac gcaggcatgt tatcctggtg aagttaagga
     2461 ggcaacc
//
LOCUS       DAOPTR010000610         2465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_33822_length_2465_cov_3.590041, whole genome shotgun sequence.
ACCESSION   DAOPTR010000610 DAOPTR010000000
VERSION     DAOPTR010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33822_length_2465_cov_3.590041"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PKI05_11240"
     CDS             complement(<1..292)
                     /locus_tag="PKI05_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52819.1"
                     /translation="MKRFWFILVNCGAILLSAGLGCRNEREANVTVLEPQKTIIVRAE
                     NTAQPGSPAQPSGEQPNAPTIIPQPGDSVGNAKPEITGATANNRQIVLGGRFR"
     gene            complement(386..865)
                     /gene="bcp"
                     /locus_tag="PKI05_11245"
     CDS             complement(386..865)
                     /gene="bcp"
                     /locus_tag="PKI05_11245"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011120596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOA52820.1"
                     /translation="MSDWVEVGQPAPDFTLPSDSGEHVRLSQFRGSPVVLYFYPKDGT
                     PGCTREACAFRDLKQELVKLGAVILGVSPDGVESHKKFREKFNLNFPLLADVDHSVAE
                     AYGAWREKTRFGKKSMGIQRSTFLIDANGVVRKVWKNVQVDGHDEQVLEALKALRQE"
     gene            1393..2454
                     /gene="aroF"
                     /locus_tag="PKI05_11250"
     CDS             1393..2454
                     /gene="aroF"
                     /locus_tag="PKI05_11250"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOA52821.1"
                     /translation="MIVVMKRGASQSQIEYVVDRVQAFGLRAHVIYGTERTVIAAIGD
                     KRDEHRASLESCPGVAEVIPILAPYKVASLEVNPEHTVVRVGPFVCGNSHIGVIAGPC
                     SVETEEQTLSTARAVKAAGATGLRGGAFKPRTSPYSFQGLKEEGLKILALARQETGLP
                     VVTEVITPSDVELVAQYADILQIGARNMQNYRLLEEAGQAGKPVLLKRGPAATAEELL
                     LAAEYVLNTGNHNVILCERGIRTFESHTRYTLSLATVVWLHQRTHLPVIVDPSHGTGH
                     TALVPQMAAAAVAAGADGLIIEVHPRPEAALSDGYQSLTFEQFRETMDLCCKVAHALG
                     RHLGSRALPTATQLVGAAC"
BASE COUNT          554 a          653 c          685 g          573 t
ORIGIN      
        1 cccggaatcg tccccccagc acgatctgac gattgttcgc cgttgcgcct gttatttccg
       61 gttttgcgtt gcccacacta tcgcccggtt gcggtatgat ggtgggggcg ttcggttgct
      121 cgccggatgg ctgggcaggt gaacctggct gggcggtatt ttcggcacga acgatgatcg
      181 tcttttgagg ttccaaaacc gttacgttcg cttctcgttc atttcggcaa cccagcccag
      241 cgcttagcag aatcgcgcca cagttgacca agatgaacca aaatcgtttc atagatcggt
      301 gttccaaatt ttgcgaacgg tcggcacagt ctggtacttg gcatgtcgtg accacccacc
      361 aagccgtcat agtgatgcct ggaaatcact cttgcctcaa tgccttcagg gcctcaagta
      421 cctgctcatc atgaccgtca acctgaacgt tcttccatac ttttctgaca acgccattgg
      481 catcgatcaa gaaggtggaa cgctggatgc ccatggattt ctttccgaag cgggtttttt
      541 ctcgccaggc accgtatgct tccgccacgc tatgatcgac gtcagcaaga aggggaaagt
      601 tcagattgaa cttctcgcga aatttcttgt gactttccac accatcgggg ctaacaccga
      661 gaatcaccgc accgagtttc accagttctt gcttcaaatc gcggaaggca caggcctcgc
      721 gcgtacatcc tggggtaccg tccttgggat aaaagtacaa gacgaccggt gacccgcgaa
      781 actggcttaa tcggacgtgt tctccactat ctgacgggag tgtaaagtcc ggcgccggct
      841 gacctacttc gacccaatcg gacacgatga atttctcctt tccacgtaag ctagggattc
      901 gtctcacaat tgtgacagtg aaattatatg ctcacggaaa agacagcgtc gaagatgagt
      961 gacacggaag ccaagaaatt ggcgcaatcc aatggacggg tcgggctttt agagtagcct
     1021 gatctttgag gtgtaccccc agacgtgggg gtagccgtag aaatcgaatc ggcctagcat
     1081 tgtcactaaa ggtttgagcg aggcttggcc gataatcact ttgtttgccg aaggaattga
     1141 ttttttgctt gaggcgactt atagttagta gagtcgttaa catccgagtg agttgatcgg
     1201 acatgagtac ctgctgcaag acctactact ggttttattt tagcccgcag ggttgcgggg
     1261 ccggaggagg tctgtagcag cgagattcga tagacgcaga cagtaaaaag ccctggcaac
     1321 ccataaaggt cgccagggct tttttgtttt tcccaagatc gtggctaacg tctgagaaag
     1381 gagctgccgc ccatgattgt cgtcatgaaa cgaggtgcat cgcaatcgca gatcgaatac
     1441 gtggttgacc gcgttcaagc ctttggactg cgtgcccatg tgatttacgg gacggagcga
     1501 acagtgattg cggccatcgg cgataagcgg gacgagcatc gtgcctcttt ggagagttgt
     1561 cccggggtgg ctgaggtgat tcccattcta gctccttaca aagtggcaag tctcgaggtc
     1621 aatccggaac acacggtggt cagggtcggc ccattcgttt gcggcaattc ccatatcggt
     1681 gtcatcgctg gaccgtgttc ggtggaaaca gaagagcaga cactatccac agcccgggcg
     1741 gttaaagccg ctggagcgac gggcttgcgc ggaggggctt tcaaacctcg aaccagtccg
     1801 tatagtttcc aaggattaaa agaggaaggc ctcaagattt tagcactggc ccggcaggaa
     1861 accggtttgc ccgtcgtgac cgaggtcatc acgccctcag atgtggaact ggtagcacaa
     1921 tacgctgata ttttgcaaat cggcgcgaga aacatgcaaa actatcggct tctggaagag
     1981 gccgggcagg cgggcaaacc cgtcctgttg aagcgagggc cggcggccac cgcagaagaa
     2041 cttctcctcg ccgccgaata tgtgctcaac accggcaacc acaacgtgat cctgtgcgaa
     2101 aggggaatcc gaacgttcga atcgcataca cgttacacac tttctttggc gactgtcgtt
     2161 tggcttcatc agcgaacgca tttgcccgta attgtggatc ccagccacgg gacgggacat
     2221 accgccctcg taccacagat ggccgccgcg gccgtagcag caggggcgga tggactcatc
     2281 attgaggttc atcctcggcc tgaggcggct ctcagcgacg gttatcaatc cctcaccttt
     2341 gagcaattca gggagacgat ggacctgtgt tgtaaggtgg ctcatgccct cggtcgacat
     2401 ctcggctcta gggccctgcc caccgccacc caactagttg gtgccgcttg ctaacgtagg
     2461 tgtgt
//
LOCUS       DAOPTR010000611         2463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_33853_length_2463_cov_2.389950, whole genome shotgun sequence.
ACCESSION   DAOPTR010000611 DAOPTR010000000
VERSION     DAOPTR010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33853_length_2463_cov_2.389950"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            391..1821
                     /locus_tag="PKI05_11255"
     CDS             391..1821
                     /locus_tag="PKI05_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52822.1"
                     /translation="MIRSLQRRQFLELTVLGTAALGMRQWAWSSQAPASKLISPGCRR
                     SKVKIAKLYLAGSGLWPHPKLDVEAERQSYEQAFKEMADQFADVDFFVDELITSPKQI
                     SDLADKLAQADGILAIHIRMGVGAMLAELYKVGRPMMIFAVPYSGHEWSGFGAVLRQA
                     EPPPMDVILSADKRDLATAVRPIRALHHLKDAKIVNVTLREPGDFHQAVKDKFGTEIK
                     HIGLEPVVALYEKVSDQDAEAEAKKWMEGAKQIVEPSPEEIFRSCKLALAFERLLDEE
                     DATVLTVDCYGTMWDRTIKLPAYPCLGFSRLNGMGLGGICESDLRCAMTHIIFQGLAG
                     KPGFISDPTVDQSTNTIILAHCMGTVKMDGVNSPDLPYKLRCVMERQEGVTPQVFVPR
                     GVRATQALLVDTNTLLYFTGEVVDAPDIERGCRTKITVRVDGDVEKLWRNWSSGLHRS
                     TCLGDLTKDLERFARLSKMRLVNELA"
     gene            2205..>2463
                     /locus_tag="PKI05_11260"
     CDS             2205..>2463
                     /locus_tag="PKI05_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOA52823.1"
                     /translation="MRYSAIRLDKAWPRKAFTLVELLVVIAIIGILIALLLPAVQAAR
                     EAARRSQCSNKLKQLGLALHNYHDAHGAFPSGTLSANPTNYY"
BASE COUNT          579 a          626 c          677 g          581 t
ORIGIN      
        1 gcctgagaac gattccgttc atagaaaggg tgcggcgcaa accccaccag cgcccgctgg
       61 ttacccctca atggcggtac ccaattcggg tccccatggt agagccgcca gggaaattct
      121 agaaacaacc gctgttcttt ttttgtctcg acgggcttga caacaatgtt tgacataaat
      181 gggcaacttt ccaaaatatc cgacaagcac tcaccgcgga ttgtatcgcc tgatttcgct
      241 tggggtcaat tgaaaaatcg taatcccttg cggcctagct tgacaactcc cacttggcaa
      301 atcatgatga cgcacgccgg gatcagggtt cggtcagagt gaattggtac ttcgaaagaa
      361 ggtttcattg ggagagtcac atagtcacat atgattcgtt cacttcagcg gcgacaattc
      421 ttggaactga cagtccttgg aacagcggcc ttggggatgc gccaatgggc gtggtcatcg
      481 caggccccgg cgagcaagct tattagtcct ggttgtcgcc gatccaaggt taagatcgcc
      541 aaattatatt tggcgggctc agggctttgg ccgcatccca agctggatgt cgaagcagaa
      601 aggcaatcgt acgagcaagc ctttaaggaa atggcggatc aattcgccga tgtcgatttc
      661 ttcgtcgatg aactcatcac atcacccaaa caaatctcgg acttggccga caaactcgct
      721 caggcggacg gcattctggc catccatatt aggatgggcg tcggggccat gttggctgaa
      781 ctttacaaag tcggtcgccc catgatgatc tttgccgttc cctactccgg ccatgagtgg
      841 tctggttttg gtgcagtcct ccgccaggcc gaaccgcccc ccatggacgt tatcctaagc
      901 gcagacaaac gcgatctggc cacggcggtt cgccccattc gggcacttca tcacctcaaa
      961 gatgccaaaa tcgttaacgt cacactccgt gagccagggg atttccatca agccgtcaag
     1021 gataaattcg gcacggaaat taaacacatc gggttggaac cggtggtggc gctctacgaa
     1081 aaagttagtg atcaggatgc cgaggctgaa gccaagaagt ggatggaagg ggccaaacag
     1141 atcgtggaac cgagcccgga ggagattttc cgctcctgca aactggctct tgcgtttgaa
     1201 cggctccttg atgaagaaga cgctacagtg ctgacggtcg attgctacgg caccatgtgg
     1261 gatcggacga ttaagctgcc ggcttatccc tgcttgggat tttctcgact caacggcatg
     1321 ggattggggg gaatctgtga gtctgatctc cgctgcgcca tgactcacat catcttccag
     1381 ggattggccg gaaagccggg atttatcagc gatcctaccg tagatcaatc gacaaacacc
     1441 atcattttgg cccattgcat gggcaccgtc aaaatggatg gtgttaattc cccggacctg
     1501 ccgtacaaac tccgctgcgt gatggagagg caagagggcg tgactcctca agtgtttgtg
     1561 cctcgtggcg tgcgggcgac gcaagcctta ttagtggata cgaataccct tctctacttc
     1621 accggtgagg ttgtcgatgc cccggacatc gaacgtggat gccgtaccaa aatcacggtt
     1681 cgcgtcgatg gtgatgtgga aaaactgtgg cggaattgga gctccggcct gcatcgctcg
     1741 acgtgcctgg gcgatttgac caaggatctg gagcgatttg cccgactttc caagatgcgc
     1801 ctggttaacg aactggccta aggcacgcag aaggttcaaa gctccatcaa tcgaaattgt
     1861 caggccgaaa ttgacgaaaa tggtcccgcc aatcgggcga cctaatggtt ttaagtttac
     1921 gagtggcgat gggtaatgtt acctgatcct ctcgcggagg gatgtgcgta gggcgtttgc
     1981 gccgtaattc tcgttgatag caagagttgg gccggaaact tgggtgaatt aggagaacaa
     2041 gagaaaccga ccggggagaa agcaaaattt gttgacagtg cttattgctt ggcgatacaa
     2101 tgcctcttgt gcgatcgggg atagcgatca gctcgctgcc caaggtgcgg attcagttca
     2161 gagttttcta agatttctct actttgtggg acggaggtct catcatgagg tacagtgcga
     2221 ttcgcttgga caaggcctgg cctcgcaagg cctttacttt ggtcgagtta ttggttgtga
     2281 ttgccattat cggcattctt attgccttgc tgctacctgc ggtgcaggca gcccgtgaag
     2341 ctgcccgaag aagtcaatgt tcgaacaagc taaaacaact gggtctggcg ctccataatt
     2401 accacgatgc ccatggtgcc ttcccctcgg gaacgctctc cgcaaatcca acaaattatt
     2461 aca
//
LOCUS       DAOPTR010000612         2459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_33924_length_2459_cov_3.348586, whole genome shotgun sequence.
ACCESSION   DAOPTR010000612 DAOPTR010000000
VERSION     DAOPTR010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33924_length_2459_cov_3.348586"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="PKI05_11265"
     CDS             complement(<1..1419)
                     /locus_tag="PKI05_11265"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOA52824.1"
                     /translation="MTERGPPPRPFEMLVVYCPSCFSSQPREPWEGRGPLRPPISALP
                     GFCGLWIPQDLRSSACQRHNIEEGQAPFTSSIGDRAAIMAGLEETIRDLCHQLDRAIR
                     HREARRVEDYLATLDVSQLETDLLLELIYTEFVARSETGESPQADEYYERFPYLKDDL
                     QELFFVHNLVAASLHSLDSTKEENDAEGEPSDLVANSFDAPATPAEGQSGSSPFETTT
                     GEGFAAEGTVSESGVRGTILGHYELLEEIGRGATAVVFKARDLRLKRYVALKVINQAS
                     WDRAARERVLAEAQAAAGLRHPHIVQIYEVGESGDTCFVAMEYLEGGSLADRPDRVWS
                     PREAARLVERIARAVQYAHEHHILHRDLKPANVLLDLDGTPKIVDFGLAKRLDEVSPN
                     GSQEDLVGTPSYMAPEQAAGEVGAVGPATDVYGLGAILYELLAGRPPFTGKNAIETLL
                     QVRHQDPVPPSRLQPYIHRDLVT"
     gene            1789..>2459
                     /locus_tag="PKI05_11270"
     CDS             1789..>2459
                     /locus_tag="PKI05_11270"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOA52825.1"
                     /translation="MDRFSGVELPRLWIHLLYVLAGVCWLMQFGLGQEPPTVQKSFAP
                     RELVQGFSNPPSCARPAAYWCWMNGYVDEPQALWEIQEFRRKGLTCLYVFDIGARDGD
                     VKVPAGPAFMGPQWVKTFANVVREAAKCDMEIGLITSSSWNAGGPWVTPEYGAMGLFT
                     TQLTFEGPADFTATLPQPELPAAASKHPAGLPPIYRDIAVLAVPAENVYDRPTFVFQL
                     APPGR"
BASE COUNT          516 a          737 c          701 g          505 t
ORIGIN      
        1 cgtcacgagg tcacgatgaa tgtacggttg cagtcgcgag ggcggaaccg ggtcctggtg
       61 gcgaacctga aggagtgtct cgatggcatt cttgccggta aagggtggcc gacctgccag
      121 cagttcgtac aggatcgccc ccaggccata aacatcggtg gcaggtccaa cagctcccac
      181 ctcgcccgcc gcttgctccg gggccatgta gctgggtgta ccaaccaaat cctcctgcga
      241 accattgggc gagacctcgt ccagcctttt cgccagtcca aaatccacga tttttggtgt
      301 gccatcaagg tccaaaagga cattggcggg tttcaggtct cgatgaagga tgtggtgctc
      361 gtgggcgtac tggacggcac gggcaattcg ttccaccagg cgtgctgcct cgcgtggcga
      421 ccacacccga tccggacgat cggccagcga tcccccctcc aaatactcca tagcgacgaa
      481 acaggtatcg ccggattcac cgacctcgta tatctgaaca atgtggggat gtcgcaaccc
      541 tgcggcggcc tgcgcttcgg cgaggacccg ctctcgcgct gcccgatccc aactcgcctg
      601 attgatgact ttaagcgcga cgtatcgctt gagacgtaag tcgcgagcct tgaagacgac
      661 cgcagtggcc cctcgcccga tctcttctag cagctcgtag tgcccaagaa ttgtgccgcg
      721 aacacccgac tcggaaactg tcccctcggc tgcgaaacct tcgccggtgg tagtttcaaa
      781 tggggatgaa cctgattggc cttcggcggg ggtcgctgga gcgtcgaacg aattggcgac
      841 taaatccgaa ggctctccct ccgcatcatt ttcttctttg gttgaatcaa gagagtgaag
      901 tgacgccgcc accaaattgt gaacgaaaaa caactcttgc aggtcatctt tcaagtaagg
      961 aaagcgttca tagtactcat cggcctgcgg cgattctccg gtttcgctac gtgccacgaa
     1021 ttctgtgtag ataagttcca gcaaaagatc agtctcaagc tggctgacgt ccagggtggc
     1081 cagatagtcc tcgacccggc gggcttcccg atgacgaatt gcccgatcaa gttggtggca
     1141 caaatcgcga atagtctctt ccaaacccgc catgattgca gcgcggtccc ctattgagga
     1201 tgtgaacggg gcttgcccct cttctatatt gtgccgctgg caggccgaag aacgcaaatc
     1261 ctgtggaatc caaagcccgc agaaccccgg caacgcagaa atcggcggac gcaggggacc
     1321 gcgtccctcc cacggttccc gcggttgcga ggaaaaacat gacggacaat agaccacgag
     1381 catctcaaac gggcgtggtg gagggccacg ctccgtcacg atcggctaac aatttattcc
     1441 aaagagtccc cctctccctc tgggagaggg ttggggtgag ggcaagcgat cgtcggaatc
     1501 gattgagaaa accggcagcg attcgagcca ccctcacccc cgacccctct cccgccgaac
     1561 gggcgagggg aaattgtgga atcggctcta actgcaaacg cccccgatcg ggaaaacggc
     1621 ggacgcgggg gactgcgtcc ctcccactgg caacggagga ctcacgatcg ggcaacttga
     1681 caatgcgctc gtcctcgctc acatttttgg cagggttact aggcccatgg tcgtttcaaa
     1741 gattcgccct taggccgtgt tcactgagaa agatcaacga ggtgtcgtat ggataggttt
     1801 tccggtgtgg agcttccgcg attgtggatt catctccttt atgtcctcgc cggggtatgc
     1861 tggttgatgc aatttgggct ggggcaggag ccaccaacgg ttcagaaaag cttcgccccg
     1921 cgagagctgg ttcaaggctt tagtaatccg ccaagttgcg cacgtcccgc cgcctactgg
     1981 tgttggatga atggctacgt ggacgaaccc caagccctct gggaaatcca ggagttccgc
     2041 cggaaaggcc tgacctgtct ttatgtcttc gacatcggtg cgcgagacgg cgatgtgaag
     2101 gtacccgccg gacccgcgtt catgggccct caatgggtca agacttttgc caatgtcgtc
     2161 cgtgaagcag cgaaatgcga catggagatc ggcctaatta cctccagcag ttggaatgcg
     2221 ggtggcccct gggtcacgcc agaatacggc gccatgggat tattcacgac gcaactcact
     2281 ttcgagggcc cggccgattt taccgccacc ttgccacagc cggagcttcc cgcagcggcc
     2341 tccaaacacc cagccggttt gcctcccatc taccgggata ttgctgtgct cgccgttccc
     2401 gcggagaacg tctatgatcg gccgacattc gtcttccaac tggcgccccc tggccgaca
//
LOCUS       DAOPTR010000613         2459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_33926_length_2459_cov_3.128536, whole genome shotgun sequence.
ACCESSION   DAOPTR010000613 DAOPTR010000000
VERSION     DAOPTR010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33926_length_2459_cov_3.128536"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1860)
                     /locus_tag="PKI05_11275"
     CDS             complement(<1..1860)
                     /locus_tag="PKI05_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011122144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOA52826.1"
                     /translation="MAAFRFDKLTIKAQEAVANAQSLATERGHPEIDPVHLLAALLSE
                     SDGIVKPLLDRIGVNWSQLNRMVEAELGHFPKVSGAAQTAPNTAFMKVLEQAQKEADT
                     LRDDYISTEHLLLGLSKIDSKARNILKLNAIGEQEILNAMRAIRGPARVEDQNPEAKF
                     QALERYGVDLVERARRGKLDPVIGRDQEIRRVMQVLSRRTKNNPVLIGDPGVGKTAIV
                     EGLAQRIADGDVPETLKNKRVIALDMGALVAGTKYRGEFEERLKAVLREVEAAGGNIV
                     LFIDELHTVVGAGRAEGGADAANLLKPALARGELRCIGATTLDEYRKYIEKDAALERR
                     FQPVYVGEPSVEDTIAILRGLKPRYEAHHGVKVKDSALVAAAQLSHRYITDRYLPDKA
                     IDLVDEAMSRLAMELKSVPVEIDQLQRRIMQLELAERQLADETEEHAKERRAEIAAEI
                     SRLREQLRELRAQWEKEKAALENIHRLRNELQQAEHEFNQLQASIKEKQSMGVIVREE
                     DYQRLYELDLRRRELAQQLEKAENNYPVTDDKRLLRLEVGPEEIAEVVSAWTGIPVSR
                     MMETERAKLLVLEERLHQRVVGQDEAVEAVANAVRRSRSGLQDPKRPIGSFIFL"
     gene            complement(2014..>2459)
                     /locus_tag="PKI05_11280"
     CDS             complement(2014..>2459)
                     /locus_tag="PKI05_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOA52827.1"
                     /translation="TGKEQKVRIEQSAGLSKEEIERMRREAELHAEEDRRKRELAELR
                     NKADNLAWQIEKLMKEHEDKLSSADREALTRAIEKARNAAKSENAGEIKSAISELEAA
                     AHAFSKTLYERAAAAAGATSTTGSGSSSSAGKKDDDTIDAEFEVK"
BASE COUNT          480 a          746 c          617 g          616 t
ORIGIN      
        1 gagaaagatg aacgacccga tgggacgttt cgggtcttgc aagccagatc ggctccgccg
       61 cacggcgttg gccacggcct ccaccgcctc atcctggccc acgactcgct gatggagtcg
      121 ctcctccagg acgagcagct ttgcacgttc tgtttccatc attcgggaaa caggaatccc
      181 ggtccaggcg ctgacgacct cggcgatctc ctcgggtccc acttccaaac gaagcaatcg
      241 cttgtcatcc gtcaccggat aattgttctc ggccttctcc aattgctgcg ctagttcgcg
      301 gcgacgtaaa tcaagttcgt agagtcgttg gtaatcttcc tcccggacaa tcacccccat
      361 ggattgcttt tctttgatac tcgcctgaag ctgattgaat tcgtgttccg cctgctgaag
      421 ttcattgcgg aggcgatgga tgttctccaa ggcggccttt tctttttccc attgagcccg
      481 cagttccctg agctgctcac ggagacgact gatttccgcg gcaatctcgg cgcgtcgttc
      541 tttggcgtgt tcctccgtct catccgccag ttgtcgttct gcgagctcta attgcataat
      601 tcgccgctgg agttgatcga tctccacggg gacgctcttc aattccatgg ccaaccggct
      661 catcgcctcg tcaaccaggt cgatggcctt atccggaaga taacgatcgg taatatagcg
      721 atgggaaagt tgtgctgccg ccactagggc ggaatccttc accttcacgc cgtgatgggc
      781 ttcatacctt ggtttgaggc cgcgaagaat ggcaatggta tcctcaacgg aaggctcgcc
      841 tacataaacc ggctggaatc gccgttccaa ggcggcatcc ttctcgatgt actttcgata
      901 ctcatccagg gttgtcgcac cgatgcaacg aagctcgccc cgcgcgagag caggcttcaa
      961 cagattggcg gcgtcagccc caccttctgc ccgaccagct cctaccaccg tatggagttc
     1021 gtcaatgaag agaacgatgt ttccccctgc agcttcgact tcccgcagaa ctgccttgag
     1081 gcgttcttca aactcgccac gatatttggt accggcaacc aatgccccca tgtcaagggc
     1141 aatgactcgc ttgttcttca acgtttcggg gacgtcgccg tccgcgatgc gctgagccaa
     1201 cccttccacg atggccgtct ttccaacacc cggatcgccg atcaacacgg gattgttttt
     1261 ggtccgtcgg gaaaggacct gcattacccg gcgaatctct tggtcccgtc caatgacggg
     1321 atcgagtttt ccccggcggg cccgctcaac aaggtccact ccataccgtt ccaaggcctg
     1381 gaatttcgcc tccggattct gatcttccac gcgagccggc ccccgaattg cccgcatcgc
     1441 attgagaatc tcctgttcgc caatggcgtt aagtttcaag atgtttcgcg ccttggaatc
     1501 aatcttggac aggcccagca acaagtgttc tgtggagata tagtcatcac gaagcgtatc
     1561 agcctctttt tgggcctgct ctaacacctt cataaaggct gtgttggggg cggtttgagc
     1621 agctcccgag acctttggaa aatgacccaa ctccgcctcg accatccggt taagttgtga
     1681 ccaattcacc cctatccgat cgagaagggg tttaacaata ccgtccgact ctgataaaag
     1741 cgctgcgagc agatgtaccg ggtcaatttc cgggtggccc ctttccgttg ccaaagactg
     1801 agcgtttgca accgcctctt gtgctttgat agtcaattta tcgaatcgaa aagctgccat
     1861 tgtttattgc tccccttgat atttcctcaa aaaattcctg actagaagcc atgaatgtct
     1921 tgaaaaaagg gccgctttcc atggtcatga cgcaagaaag cggcccttcg cgtggctcaa
     1981 aaactcgagg gacgcgaatc aacgctaccg acgttatttc acctcgaatt ccgcatcaat
     2041 ggtatcgtca tccttcttgc cagcactgct ggacgatcct gaaccggttg tcgatgtggc
     2101 cccggcagcg gccgccgctc gctcgtagag cgtcttgcta aaagcatggg cggcagcctc
     2161 cagttcgctg atcgctgact tgatttcccc ggcgttctcg ctcttggccg catttcgggc
     2221 cttttcgata gcacgggtca atgcctcgcg atcggcactg gagagcttgt cctcatgctc
     2281 tttcatgagt ttttcaatct gccaggcgag attatccgcc ttgttgcgga gctcggccaa
     2341 ttcgcgcttg cggcgatcct cctcggcatg gagttcggcc tcgcgccgca tcctttcgat
     2401 ctcttccttg gacaaaccag cggactgctc gatgcggact ttttgttcct tgcccgtac
//
LOCUS       DAOPTR010000614         2457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_33956_length_2457_cov_3.313072, whole genome shotgun sequence.
ACCESSION   DAOPTR010000614 DAOPTR010000000
VERSION     DAOPTR010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33956_length_2457_cov_3.313072"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2151)
                     /locus_tag="PKI05_11285"
     CDS             complement(<1..2151)
                     /locus_tag="PKI05_11285"
                     /inference="COORDINATES: protein motif:HMM:NF039533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOA52828.1"
                     /translation="MDGTSTVPDLIIYNGIAGVSERIELVPTGPGDGQLRVTNAADGS
                     LITVINYISNTGFIINGNDGSAGDTDSLTLHGTSGDDAIHIVDAQNANVSGIYSVQFT
                     NFAVLNVAGGDGYDTFHVNPSTLQVVIDGGTPDGVIQSDGDHLYVWPGLASYHLILGP
                     EGDNGTVAVTGAAPITFDRIEGLGFQGILYTLPDLNEPNDTIAEATILGSIPHLTLRD
                     LTIHTNEWNTTNDDYFQITARDSGALLVNVMFTDDGIGGNQVAGALSAELLDRLGNVI
                     ATAVPTAYGVQIVAPVVSQQRYYVRVFSTDNDPNTYTLEIENFAAPAPTAIVMHPAYD
                     TGRSDSDGITAADDPVMLIQAWLSDLAEKGVPVLSAADVAAGLPGLAVEVFVNGISAG
                     FADPFGPSNNVFAFALPSGLLSAGLPGGYAGNPTAFGWLNLISAAVRVFDAQTVPASA
                     TRELGIPLPVTYDPNVPDASLLTLALATSCDSGVVGDGVTNIRTPTFQGVSEINTIVR
                     LYANGQLVGQATTGSDLSDGILGNGLGVFSITAAPLTDGVYFYAVTVEDVAGNITGIV
                     DSPIVILQIDTTPPQIPTIDLLDQFDTGWSDKDNVTKGDLTQGPGVVDVRVSGELGTT
                     AVIKDGETVIDTFVFAAAFMTRTLTLGEGPHPLTIEVTDTAGNRTQSEQLLITVDTIA
                     PDPASISLAPYSDSGIPGDGITNVSAPAFVGLAEA"
     gene            2191..>2457
                     /locus_tag="PKI05_11290"
     CDS             2191..>2457
                     /locus_tag="PKI05_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52829.1"
                     /translation="MRRIVGKRCDDEGIHIVRTDTRRACRVVAEDIEIGYTEKLNWRI
                     PVNRERKRVVAGTHNLKEVVLVHAGIVVVAVNVDIRAVGLKGEIA"
BASE COUNT          575 a          695 c          672 g          515 t
ORIGIN      
        1 ggcctccgcc aaaccaacaa atgccggagc cgaaacattc gtgataccgt cgcccggtat
       61 gccagagtcg ctgtatggag cgagactaat gctggcggga tccggagcaa ttgtatccac
      121 ggtgatcaaa agttgctcag attgagtccg atttcctgca gtgtcagtga cctcaatggt
      181 gagaggatgc ggtccctctc cgagggtcag tgtccgcgtc atgaatgccg cggcaaacac
      241 gaaggtatcg atgacggttt cgccatcctt gatcactgcc gttgttccca gctcgccaga
      301 aacccgaaca tctaccacac caggcccctg cgtcaaatcg cccttcgtga cgttgtcctt
      361 gtcggaccag cccgtgtcga actggtccaa cagatcaatc gttggaatct gaggcggcgt
      421 ggtatcaatt tggaggataa cgatagggct atcaacgatg cccgtgatgt tgcccgccac
      481 gtcttccacg gtcaccgcat agaagtaaac cccgtcagtc agtggtgctg ctgtgataga
      541 aaacactccc agaccattac cgagtatccc atcgctcaga tcggagccgg ttgttgcctg
      601 gcccacaagt tggccattgg catacaatcg gacgatggta ttaatctcgc ttacaccttg
      661 gaacgtgggg gtgcgaatgt tggtaacacc atcgccgaca actccgctgt cacatgatgt
      721 ggccagcgcc aacgtgagga gactcgcatc ggggacattg gggtcataag ttacgggaag
      781 aggaatcccc agttctcgtg tggccgaggc tggaacggtc tgggcatcga acacccgcac
      841 cgccgcactg atcagattga gccaaccgaa agccgtggga ttacccgcgt aaccgcccgg
      901 caagccagca gacaaaagtc cgctaggcaa agcaaaggca aacacgttgt tcgacgggcc
      961 aaatgggtcg gcaaagccag cgctgatccc gttgacaaac acttcgacgg ccagaccagg
     1021 caacccagcc gcgacgtctg ccgcgctaag aaccgggaca cctttctccg ccaggtccga
     1081 caaccacgcc tgaatcagca tgactggatc atcggcagcc gtgatgccat cggaatcgct
     1141 gcggccggta tcatacgccg gatgcatcac gatcgcggtc ggggccgggg ccgcgaagtt
     1201 ctcaatctcc aacgtgtaag tgttgggatc gttgtccgta ctgaagactc tcacatagta
     1261 tcgctgttgc gacacgaccg gtgcgacaat ctggacacca taagcagtcg ggacggccgt
     1321 ggcaatcacg ttgcccagcc tgtccagaag ctccgccgaa agcgcgcccg cgacctggtt
     1381 tccaccaatc ccatcgtccg tgaacatgac attcaccaat aaggccccac tgtcgcgggc
     1441 ggtgatctgg aaataatcat cgtttgtggt gttccattca ttggtgtgaa ttgtcaagtc
     1501 ccgaagagtt aaatgtggaa tggaaccaag aatggtggct tctgcgatcg tgtcgttcgg
     1561 ttcgttcaag tcaggtaacg tataaaggat gccttggaaa ccaaggcctt cgatccggtc
     1621 aaatgtaatc ggggcggccc ctgtcaccgc cactgtgccg ttatcaccct ctgggcccaa
     1681 gatgagatgg tagcttgcca atcctggcca tacgtacaga tgatcgccat cagattggat
     1741 caccccatca ggggtgcccc catcaatcac cacctgcaac gtggacggat tgacgtggaa
     1801 cgtgtcgtag ccgtcgccgc ccgccacgtt tagcacagca aaattggtaa actgaaccga
     1861 gtagattccg ctgacgttcg cgttttgagc atcgacaatg tggatcgcat catccccgga
     1921 agtgccgtgc aaggtcaacg aatctgtatc gccggccgaa ccgtcgttgc cattgatgat
     1981 gaaaccggtg ttggaaatgt aattgatcac tgtaattagg gaaccgtccg cggcattggt
     2041 cacccgcaac tggccatcgc caggaccggt cggaaccaac tcgatgcgtt ctgaaacacc
     2101 ggcaattcca ttatagatga tcagatcggg cacggtactg gtgccatcca ttggattccc
     2161 ctcatcaaaa ctaatgttaa tgccccagcc ttgcggcgaa ttgtcggcaa acggtgtgat
     2221 gacgagggta tccacatcgt tcggaccgac actcggcgtg cctgtcgcgt cgtcgccgaa
     2281 gatattgaga tcggttacac cgaaaaactg aattggcgca ttcccgttaa tagagagcgc
     2341 aaacgagtgg tagccggtac ccacaatctc aaagaggtcg ttttggtcca cgccgggatc
     2401 gttgttgtcg ccgtaaatgt tgacattcgc gccgttggcc tcaagggtga aatcgcg
//
LOCUS       DAOPTR010000615         2457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_33961_length_2457_cov_2.732306, whole genome shotgun sequence.
ACCESSION   DAOPTR010000615 DAOPTR010000000
VERSION     DAOPTR010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33961_length_2457_cov_2.732306"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="PKI05_11295"
     CDS             <1..862
                     /locus_tag="PKI05_11295"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOA52830.1"
                     /translation="VDLFARLAKEYASSPFRPEAEYHLAGKFYNQQDFAQAALHYHEA
                     FQKAENPELKEKAVHRLGWCYFKMGQYDNAKQTFAYQREAFPQGSLFPEAVFMEAESL
                     FQLGKYGEALATYERLKPLDTETFEVLRLLHAGQAASRLNQWEKAFSFFNQCLTKFPS
                     VPEISQVEFEIGYIYYQQNDFKQAVEHFQRVVQKATDETAARAQFMIGESEFQQKEYD
                     NAVKSFFRVAYGFNSPTWQAAALYEAARCFEMLKKPEQAAQIYQELIEKFPQSDRIAD
                     AKAKLQMLRQ"
     gene            1000..1989
                     /locus_tag="PKI05_11300"
     CDS             1000..1989
                     /locus_tag="PKI05_11300"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOA52831.1"
                     /translation="MAIGTMLFVGCAVALSPPMVCAQLPGGPPRPADGAPGTSEPVNP
                     SGSQPPDWTEADQKAAEALAAPEVPEESAPQSPAQQQVPQINVLDLAFRGGVLMIPIT
                     LMSVLTVIFGLERALGLRRRKIVPNQLIRGLGAASVEKRGFDPRAVYRLAQRYPSAAS
                     NVLKAMLLKVGRPLPEIEQAMRDATEREADRLYGNVRFLTLSAAVTPLLGLLGTVQGM
                     IQAFFVTSHLPTGADRAEMLAQGIYTALVTTFAGLCVAIPASVLAHYFEGRIQKLLRE
                     LNESLLGLLPQLESFEGRLRIDADQLSLSDAPPKRDVPQPPPPPAPTRLGAVP"
     gene            1986..2441
                     /locus_tag="PKI05_11305"
     CDS             1986..2441
                     /locus_tag="PKI05_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOA52832.1"
                     /translation="MSVLIRNKSRALESLTMTPLIDVVFNMLIFFLIASKFAEEERQL
                     PVKLPNASEAQPLVSKPRELFINIDQNGRFYVGGKTLTLAQLEKMLLEAWSANPSRTT
                     VISRADERCRLQPVVAALNACKKANISDYRLATRKTGPPAEAASSPPSP"
BASE COUNT          553 a          656 c          694 g          554 t
ORIGIN      
        1 tgttgatttg ttcgcgcggt tggcgaagga gtatgcctca agtccgttca gacctgaggc
       61 cgagtatcat ctggctggta agttttacaa tcagcaggat ttcgcgcagg cagcgctgca
      121 ttatcacgag gcgttccaga aggccgagaa ccctgaactc aaggaaaagg ctgtccatcg
      181 cctcggttgg tgctatttca agatggggca atatgacaac gccaaacaga cattcgctta
      241 tcagagagaa gcttttccgc agggttcgct gttccccgag gccgtgttca tggaggcgga
      301 atcgctcttt cagttgggta aatatggcga ggccttggca acttatgagc ggctgaagcc
      361 actggatacc gagactttcg aggttctgcg tctccttcat gcaggtcagg cggcctcgcg
      421 gttgaatcaa tgggagaagg ccttctcgtt ctttaaccag tgcctgacga agtttccttc
      481 tgtcccggaa atttcccagg tcgagttcga aatcggttac atttactatc agcagaatga
      541 tttcaaacaa gccgtggagc attttcagcg ggtagttcaa aaggccaccg acgaaacggc
      601 agcccgcgct caattcatga ttggagaaag cgagttccaa caaaaagagt acgacaatgc
      661 ggtgaagagc ttcttccgcg tggcctacgg ttttaatagt cccacatggc aggcagcggc
      721 actgtacgaa gccgcacgct gctttgagat gttaaagaag cccgaacaag cggcgcagat
      781 ataccaggag ttaatcgaaa aatttccgca gagtgatcgc atcgccgacg ctaaggctaa
      841 actccagatg ctcaggcagt aacgggataa ccgataaaaa gaaggtatgg gtagaaaccc
      901 gtcgccttct gccgtcggtt actgatacat aagcgagaac cgaaaatgtc gcgttccaga
      961 ctcatcagat cttggatatt ccagcaaaga tgtccgctgg tggccatcgg cacaatgttg
     1021 ttcgtagggt gtgccgtggc gttatctccc ccgatggtgt gtgctcagct cccagggggc
     1081 ccaccccggc cggcggatgg tgctccaggt acaagcgaac cggtcaaccc atccggtagc
     1141 cagccgcctg attggaccga ggctgatcaa aaggctgcag aggccctggc tgcccccgaa
     1201 gtccccgagg aatctgcccc gcaatccccg gctcagcagc aggttccgca gattaacgtg
     1261 ctagatttgg ccttccgcgg cggtgtgttg atgattccca tcacactgat gtcggttttg
     1321 accgtcatct ttggtttgga gcgggccctt gggttgcggc ggcgaaagat tgttcctaat
     1381 cagttgatac gaggattggg cgcggccagt gtggaaaaac gggggtttga tccgcgggcg
     1441 gtctatcgtc tcgcgcagcg gtacccgtcc gcggcttcca atgtgctcaa ggcaatgctg
     1501 ctcaaagtgg gacgcccttt gcccgaaatc gagcaggcga tgcgcgatgc cacggaacgg
     1561 gaggccgatc gcctttacgg aaatgtccgt ttcctcacgc tctcggcggc agtgactccc
     1621 ttgctggggt tattggggac tgtccaaggc atgatccagg ccttctttgt cacgtcacac
     1681 ttaccgacgg gtgcagatcg ggcggaaatg ctcgcccaag ggatctatac agctttggtg
     1741 acaacttttg ccgggctgtg cgtggccatt cccgcttccg ttttggctca ttattttgag
     1801 ggacgtattc aaaagctttt gagagaactg aacgagtcgc tccttgggct cctgccccag
     1861 ttggagtcgt ttgagggtcg gcttcgcatt gatgccgatc agttgtcgtt gagcgacgcc
     1921 ccacccaagc gggatgtgcc tcaaccgccg ccaccgcctg cgccaacgcg tttaggagcc
     1981 gttccatgag cgttctcatc cgcaataaaa gccgtgccct cgaatccctc acgatgacac
     2041 cactcatcga cgtggtgttt aacatgctca tcttctttct catcgcaagc aagtttgccg
     2101 aggaagaacg gcaactgcct gtgaaactgc ccaatgccag cgaggctcag ccgttagtca
     2161 gtaagccgag agagctcttc atcaacattg accaaaacgg cagattttat gtggggggaa
     2221 aaacgctcac gcttgcgcaa ctggaaaaaa tgctcttgga agcgtggtcg gctaacccaa
     2281 gtcgaacaac cgtcattagt cgggctgacg aacgctgtcg attacaaccg gtggttgctg
     2341 ccttaaacgc atgcaaaaag gccaacatat cagattatcg tttggcgaca cgtaagacgg
     2401 gcccgcccgc ggaagcggcg tcaagccctc cgtcgccgta acgttaagat gtgatgg
//
LOCUS       DAOPTR010000616         2456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_33977_length_2456_cov_3.609746, whole genome shotgun sequence.
ACCESSION   DAOPTR010000616 DAOPTR010000000
VERSION     DAOPTR010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33977_length_2456_cov_3.609746"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PKI05_11310"
     CDS             complement(<1..327)
                     /locus_tag="PKI05_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52833.1"
                     /translation="MPEKSNLPKKPTSEKVFVTVSLFQGFILDLRVLYSLDGRVYLAC
                     LQQHLSPTRMFKHTVPVGVFGRMQLGFSVTVSSCLRVIREPPHVITEMVLATEEERIV
                     SQTKLIA"
     gene            552..>2456
                     /locus_tag="PKI05_11315"
     CDS             552..>2456
                     /locus_tag="PKI05_11315"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HOA52834.1"
                     /translation="MPIRFLYGLKAALGTRFRKTGVRPAKRRVQSLFRLARGVEPLES
                     RHLLSASPLNSFAGDVVAGWALGGQAFDDARSIAVDSQQNVIVAGSTFSGGWTSAGYD
                     TTWGGEGDAYVAKFTRDGQHLWSTYLGGAYDDGITALAVDEEGFIYVAGWTRSSGWVS
                     GGYDTSYGGSGDGFVVKLSPAGEYLWATYLGGTNWDAISALCLTPDGKIAVAGTTSSS
                     GWIRKGFDATYNGGTFDAFAAVLTRDGEFLWSTYLGGTGWDYGYAVSTTAEAVYVAGK
                     TSSANWVSQGFDVIYAGGNFDGYLVRLSLAGQHVWSTYLGGTGEDTVASLVSDSGIVW
                     AAGSTTSADLPFASVQEGQDGWDGFVVAVQENGQPLWGTRLGGTATDQATAIARAPSS
                     GLIVVGTTTSDGWLSGGRRDVPSGESDGFIARLCATSGAVLWGSYFGGAQEDVPLAVT
                     LGTNGSVLVAGMTDLSDWLPDEPWGNLGEMNAFVAVITANRAPEITAFSADRLTIAAP
                     TFVELRVLGISDGDGPADVSGVLFYRDTNGDGLWNEPDRLLGETRDIIDGKATWILEI
                     TEGAWPLGQHTVFAVAYDHAGQTSHPAVLNLTVTKLVELGPVDYTTVTENEFSAGVVV
                     YHFLAVHDALL"
BASE COUNT          474 a          632 c          781 g          569 t
ORIGIN      
        1 ggcgatcaac ttggtctggg acacgattcg ctcctcctcg gttgctagaa ccatctcggt
       61 aataacgtgg gggggttccc ttataacgcg aaggcacgac gaaaccgtga ccgagaaccc
      121 caattgcatc cggccgaata cccccacggg cacggtatgc ttgaacattc gggttggcga
      181 aagatgttgt tgtaggcagg ctagataaac tcttccgtca agagaatata acacacgcaa
      241 atcgaggatg aatccttgga aaagtgaaac ggttacaaaa accttttcgc tcgtcggttt
      301 ctttggcaaa ttcgattttt ccggcatgaa cgccgtttct ggtgcatttg atacgtcgcc
      361 tcgggaacgt ggcttgtgat gtgatctccg atcgtgtcaa ggtttgcccg accacgacag
      421 cgcgtctttg cccaatgccg gccggttggg ctgccttact tgacgccgcc ccatcgagcg
      481 aacatactat gccgtaattt ccaagtaacg caccgtaaaa cctgagtgat tcattatgaa
      541 aggcaggtct catgcctatt cggttcctct atggattgaa agcggcgttg gggacgaggt
      601 tcagaaaaac tggtgtgcgt cctgccaagc ggcgagttca aagccttttt cgccttgccc
      661 gcggtgtgga acctttggaa agtcgtcacc ttttgtctgc ctcgcctttg aacagtttcg
      721 cgggcgatgt cgtggccggt tgggcgttgg gtggccaggc ctttgacgat gcccgctcga
      781 ttgccgtcga ttcacagcaa aacgtcatcg tggcaggcag caccttctcg ggagggtgga
      841 cgtcggccgg atatgacacc acctggggcg gcgagggcga tgcctatgtt gcgaagttta
      901 cccgggatgg gcagcatctg tggtccactt acctgggcgg agcttacgac gacggaatca
      961 cggccctggc cgttgacgag gaaggattca tctatgtcgc agggtggaca cgctcatctg
     1021 gttgggtttc tggcggatac gatacctcat acggcggttc cggggatggg ttcgttgtta
     1081 agttgagtcc ggcgggcgag tatctttggg ccacctatct cgggggaacc aactgggatg
     1141 caatctcggc cctgtgccta acgcccgacg gcaagatcgc cgtggcggga acaacaagct
     1201 cctccggctg gattcggaag ggcttcgacg cgacgtacaa cgggggcact tttgacgcat
     1261 ttgccgccgt gctaacgaga gatggcgagt ttctttggtc aacctacttg ggcgggactg
     1321 gctgggacta cggttacgct gtgagtacca cggcggaggc cgtgtatgtg gccggcaaaa
     1381 cctcctcggc caattgggtt tcccaaggat tcgatgtgat ctacgccggt ggcaactttg
     1441 acggctatct tgtccgactc tcgctggccg ggcagcacgt gtggtcaact tatctggggg
     1501 gaacagggga ggataccgtc gcctcactgg tatctgacag tggcattgtg tgggcggcgg
     1561 gtagtaccac ctcggcggac ttgccttttg ccagtgttca agagggacaa gacggttggg
     1621 acgggtttgt tgtggctgtg caggaaaacg gccaaccgct gtggggtacc cgtctcggcg
     1681 gaacggcgac ggaccaggcc acggctatcg cgcgggcccc atcctcgggc ttgatcgtcg
     1741 tggggaccac gacatcggat ggttggcttt ctggtggccg gcgagatgtc cccagcggtg
     1801 aaagcgatgg gtttatcgcc cgactttgtg cgacaagcgg cgccgttctg tgggggagct
     1861 actttggcgg ggctcaagag gatgttccgc tggcggtgac attgggcaca aacggaagtg
     1921 tacttgtcgc cgggatgacg gatctatccg attggttgcc cgacgagccc tggggtaatc
     1981 tgggagagat gaacgccttt gtcgcggtga tcacggccaa tcgggccccg gagattactg
     2041 ctttttctgc cgaccgacta acgatcgcag cccctacttt cgtggaactt cgcgtcttgg
     2101 ggatcagcga cggtgatggc ccggcagacg tgagtggtgt tctcttctac cgagacacga
     2161 acggcgacgg tctttggaac gagccagatc ggctcctggg agagacgcgt gacatcattg
     2221 acggaaaggc cacgtggata cttgagatca ccgagggtgc gtggcctctc ggtcagcaca
     2281 cggtgtttgc cgtggcgtac gatcacgcag gtcagacgag tcatcctgcg gtgttgaatt
     2341 tgaccgtgac aaaactggtg gaattaggcc cggttgatta cacaaccgtc acagagaacg
     2401 agttttctgc gggtgtggtg gtttatcatt tccttgcggt tcacgacgcg cttctt
//
LOCUS       DAOPTR010000617         2455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_34001_length_2455_cov_3.359583, whole genome shotgun sequence.
ACCESSION   DAOPTR010000617 DAOPTR010000000
VERSION     DAOPTR010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34001_length_2455_cov_3.359583"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1807)
                     /locus_tag="PKI05_11320"
     CDS             complement(<1..1807)
                     /locus_tag="PKI05_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52835.1"
                     /translation="MSLSSVIGRLIGLSQLESIETIGLRWNANWMVGGSGWLFFALLI
                     VVGLVVFFYTRQSLGQRRLARVLLPVARSLALVGLVVILAQPVIVMKVRHAVRPYLWF
                     VIDDTASMAIRDEIASQEDKTEQFREPANPNSTGVPGVFGNAQEVSRTEKVKEVLFGR
                     GEKTLAELAKFYRLRFFAMRRIDGLDLLPQSDSLDAASLNSLRDELKASGDMTALGAA
                     LADVLRRKAGVQLAGIALVSDFNHNSGPPPEVFASRLDVPIFSIGVGRPESQDVAVDL
                     QLPPFMKKNERSALTVVLRQQGFAGEQVQLRVSVQRPGQPPSEQGPGVPKSPSTSGGE
                     ELSTSSGNDGSLILERTVNLSDRVVTIEVPFTPAETGQHLFVAEADVLPTEKLPQNNR
                     VTRDTFVREYFVRVLFIEDEPTWEWRFMREVFHRDPLVGPEGFRTYLRSADPQVRERN
                     PLFVKSLAIPRRDFFTNDVIILGDIPVSAMSPATAEMIQEFVNDMGGGLVFCVGARYG
                     PGQWTETPLGQLLPVEVPPLTRLRETTFAPQLTAEASLFDFMRLGESQEESAKAWNNL
                     GKLAWYYPVQRVRPLATVLACHPYDTCLDGKTRQPL"
     gene            complement(1804..>2455)
                     /locus_tag="PKI05_11325"
     CDS             complement(1804..>2455)
                     /locus_tag="PKI05_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52836.1"
                     /translation="RGQVVFVSGGLSREWSSLSASYAIVMFDRLLQNLIERSIPRRNL
                     ETVENYRVIVNDPNTAWRLVAPDGTVTPISVGAVGPQQFGIQLNGFSQRGLHRLAPGR
                     LLASDTQLASGPADAQMQPANSSELASREGNLSAEKEILLGIRGPREESLPDYVSESE
                     LEGILGGHGVLSSDNGFLQAANSHEWWPWFVAAALLGLLAELGILSNLRSRNVVSS"
BASE COUNT          592 a          702 c          663 g          498 t
ORIGIN      
        1 tgagcggttg ccgagtcttc ccgtcaagac acgtatcgta aggatgacag gccaatacgg
       61 tggccagggg gcgaacacgc tgtacgggat aataccaagc gagctttccg agattgttcc
      121 atgccttcgc ggattcttcc tgggattctc ccaagcgcat gaaatcgaaa agacttgcct
      181 cggccgtcaa ctgcggggca aatgtcgttt cccgcagacg tgtcagcggt ggaacttcaa
      241 ccggcagcaa ttgtcccagt ggcgtttcag tccattgacc agggccgtag cgtgctccga
      301 cacagaacac cagaccaccg cccatgtcgt ttacgaactc ttggatcatt tccgccgtgg
      361 caggactcat tgccgagaca ggaatatctc ccaggataat cacgtcattc gtgaaaaagt
      421 cgcggcgggg aatggcaagg ctttttacaa agagtgggtt cctttcgcgg acctgcggat
      481 cagcggaccg caagtaggtc cgaaaaccct ctggtccgac aaggggatcg cggtgaaaga
      541 cctcgcgcat gaacctccat tcccaggtcg gttcgtcctc gatgaaaagg acgcggacga
      601 agtactctcg cacaaaagta tcccgagtca cgcggttgtt ttgaggtaat ttttccgtgg
      661 gtaagacgtc ggcctctgcc acgaaaagat gctgtcccgt ttcggcagga gtaaatggga
      721 cctctatcgt gacaacccgg tcgctcaggt taactgttcg ctccaagatc aatgatccgt
      781 cgtttccgga agaggttgac agttcctcgc cgccggatgt cgaaggactt ttcggcaccc
      841 cggggccttg ctcacttggg ggctgtcccg gtctctggac tgacactctt aattggacct
      901 gctctccggc gaacccctgc tgacgaagca caaccgtcaa ggcagacctt tcgttttttt
      961 tcataaacgg cggcaattgc aaatccaccg caacatcctg ggactccggt cgtccgaccc
     1021 cgatggaaaa tatgggcaca tccaggcgag aggcaaaaac ctccggcggt ggtccagagt
     1081 tgtggttgaa atcactgacc agcgcaatac cagccaactg aactccggcc ttccgcctaa
     1141 ggacatcggc cagagcggct cccaaggccg tcatgtcacc actggccttc agttcgtcgc
     1201 gaaggctatt taatgaagcc gcatcaagag agtccgactg tggcagcaga tcgagcccgt
     1261 caatccgacg catcgcaaaa aaccgaaggc gataaaattt ggccagctca gccaaggttt
     1321 tttcgccacg gccaaaaagg acttctttaa ccttctcagt ccgtgaaacc tcttgggcat
     1381 tgccgaagac gccggggaca ccggtcgagt ttgggttagc gggctctcgg aattgttctg
     1441 ttttatcctc ctgtgatgca atttcatcgc ggatggccat actcgccgtg tcgtcaatga
     1501 caaaccacaa ataaggacga acggcgtggc gaaccttcat tacgatcaca ggctgggcga
     1561 ggatcacgac caggccgacc aaggccaatg atcgggccac gggcaaaaga acacgggcca
     1621 aacgtcgctg gcctaacgat tgccgtgtgt agaaaaacac gaccaatccc actacgataa
     1681 gtagcgcaaa gaagagccaa ccgctaccgc ctaccatcca gttggcattc cagcggagcc
     1741 cgatcgtctc gatcgactcc aactgagaaa gccctatcag ccgtccaata accgaggata
     1801 gactcacgat gaaaccacgt tgcgcgaacg aaggttgctt aggattccca attcagctaa
     1861 gagtcccaac agtgccgcgg cgacgaacca tggccaccat tcgtggctat tggccgcttg
     1921 caaaaagccg ttgtccgaag aaagcactcc atgaccgccg agaatgcctt cgagctccga
     1981 ttccgaaaca taatcgggca acgattcttc acgaggtccg cgaatgccca ggagaatttc
     2041 cttttcggcg gatagattcc cctcccggga agcaagctcg gaggaatttg ccggttgcat
     2101 ctgagcatca gccgggccgg aagcaagttg ggtgtcggaa gccaataacc tacccggtgc
     2161 caagcgatgc aacccacgct gagaaaatcc gttcagttgt atgccgaatt gttgtggtcc
     2221 cacggcgccc acagaaatgg gggtcacggt accatcgggc gccacaagac gccacgcggt
     2281 attcgggtca tttacaatga cacggtagtt ttccacagtt tcgaggttcc gtcgcggaat
     2341 ggagcgctcg ataaggttct ggagcaatcg gtcgaacatg acgattgcgt aactggccga
     2401 gaggctgctc cactcgcgtg agaggccacc gctgacgaaa acaacctgtc cccgg
//
LOCUS       DAOPTR010000618         2449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_34112_length_2449_cov_2.548454, whole genome shotgun sequence.
ACCESSION   DAOPTR010000618 DAOPTR010000000
VERSION     DAOPTR010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34112_length_2449_cov_2.548454"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..777)
                     /locus_tag="PKI05_11330"
     CDS             complement(46..777)
                     /locus_tag="PKI05_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOA52837.1"
                     /translation="MSERERFSGSDSDQAPTIAGINSDRVTETLPSHPRVLVAPLHYE
                     PRYSYPLLVWLHGEESEGERQLLEVMPQLDLQYYVAVAPRGVGSNEDFGSNWPQTEEG
                     IVQAASCVFHAMTSAEKRFRIARDKVFIAGHQSGGTMAIRLAWRYPDLFAGAVSLCGP
                     IARTHRPLAQFAKIRRLPVLLTIGRDCDCYGPCQAAEDLKFLHAAGINVMLRQYPGCS
                     ELSGYMLADVNRWMMEIVAGTQIVS"
     gene            complement(1121..2065)
                     /gene="mdh"
                     /locus_tag="PKI05_11335"
     CDS             complement(1121..2065)
                     /gene="mdh"
                     /locus_tag="PKI05_11335"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOA52838.1"
                     /translation="MRRAKISIIGAGNVGATTAHWCAAAELGDIVLLDIPEVGDMPKG
                     KALDLMQAGPIMGYDAKITGTSNYWDTAGSDVVVITAGIPRKPGMSRDDLLSTNAKIV
                     GAVAEQIKKTSPDAIVIVVSNPLDAMVQRVWQVTGFPPYRVMGQAGVLDTARFRTFVA
                     MELGVSVEDVSAMLLGGHGDTMVPLASCSAVSGIPLTQLLPPDRIAAIVQRTRDGGAE
                     IVQLLQKGSAYYAPAAATAQMVEAIIKDKKRLLPCAAYCDREYGVGGYYIGVPVILGK
                     DGVEKIIELELTPEERAAFEKSVEAVKNLVQTMNRLLR"
BASE COUNT          606 a          731 c          599 g          513 t
ORIGIN      
        1 aaagctggac cagcagttac caaacgctct cgaaatgttc gcccattagc tgacgatttg
       61 cgttccggca acaatttcca tcatccaccg gttgacgtcg gctaacatat agccggaaag
      121 ctccgagcag cccgggtatt gacgcagcat cacattgatg cccgccgcat gcaagaactt
      181 gaggtcctcg gcggcctgac acggaccgta acaatcacaa tcccggccga tagtcaacaa
      241 gaccggcaaa cgacggatct tggcaaactg ggctagcggg cgatgtgttc tcgcaatggg
      301 cccacacaat gaaactgccc cggcaaaaag atcagggtaa cgccaggcca aacggatggc
      361 catcgttccc cccgattggt gacccgcgat gaaaaccttg tcccgcgcaa tgcggaatcg
      421 cttttccgcg gatgtcatgg catggaacac acaactggcc gcctgaacga taccttcttc
      481 cgtctgcggc cagttggaac cgaaatcctc atttgagccc acaccccgcg gtgcgactgc
      541 aacgtaatat tggaggtcta attggggcat cacttccagc aattggcgtt cgccctcact
      601 ttcttcacca tggagccaaa ccaacagtgg ataactgtag cgcggctcat aatgcagagg
      661 ggctactaag acacgtggat gtgacggaag cgtttctgtt acacggtcac tgtttattcc
      721 tgctatcgtt ggtgcctgat cagagtccga accagaaaag cgctcacgtt cactcattat
      781 cacaaccacc ttgtcagggg accgattgat acttccggca acactgttcc caccgcagca
      841 ggaacaactc caccctcaca agctccccgt taaacgagag gccgtcacaa ggtaacccgt
      901 ggcctcaaac cggggtgcga ataaaaactg tactgcgatt caccattcgt gtcaatcgtg
      961 atctgaagag cacataaacg accatggtaa acaacacagc cacgcaaaac caaacccact
     1021 tgatcaacca attcttcaac gcgagatacc gcgggtcgcg gcatatcggg tcccacgttg
     1081 tccccgttta cacaacttac ccacgtttac cagcaccttg tcatcgcaat aatctattca
     1141 tggtttgaac aagattcttg accgcctcaa cgctcttctc aaaagccgct cgttcttccg
     1201 gagtcagctc taactcgatg attttctcga cgccatcctt gcccaggatg actggtacac
     1261 cgatgtagta accaccgaca ccgtactcgc ggtcacagta ggcggcacat ggcagcagcc
     1321 gctttttgtc cttgatgatc gcctcaacca tttgggccgt tgcggcggca ggagcataat
     1381 aggcacttcc cttctgaagg agttggacga tctcggcccc accatcccgg gtccgctgca
     1441 caatggctgc gatgcgatca ggcggtagca gttgggtgag gggaatcccg ctcacagcac
     1501 tgcaactggc cagcggaacc atcgtatcgc catggccacc caggagcatg gccgaaacat
     1561 cctccacact cacgccaagc tccatggcca caaaggtgcg aaagcgtgcc gtgtctagaa
     1621 caccggcctg gcccatcacg cgatacggag gaaaccccgt tacctgccag actctttgaa
     1681 ccatggcatc cagcggattg ctgacgacga tgacaatggc atccggactg gtcttcttga
     1741 tttgttcggc cacagcaccg acgattttcg cattcgtcga gagaagatca tcccggctca
     1801 tcccaggttt tcgcgggata ccggccgtaa tcactacgac atcgctgccg gccgtgtccc
     1861 aataattgct ggtccccgtg atctttgcgt cgtaacccat aatgggacca gcctgcataa
     1921 gatcgagggc cttacccttg ggcatgtctc cgacttcggg aatatccagc aacacaatat
     1981 cgccaagctc cgccgccgcg caccagtggg ccgtcgtggc acccacattg cccgcaccaa
     2041 taatgctgat ttttgctcgc cgcatgacac actcctcaag ataaccaagt catttgacat
     2101 acggggtgat gccccggtcg caatcctaga ctggacactg ctaaaggctt ttattctttc
     2161 ccgaatcggc caaaaagaaa aggcatggct gcgatcggtg attctttcgc acatatcccg
     2221 cactgtggtc acccctttgc actaagccag gcgcaaagaa cgggcctctt gtcgcgcaca
     2281 tcggaagatg ttacgtaaaa aggtttggga atcgtcgaag cgtggccaat tcctcgtgat
     2341 ttgtggaatc agtaaacaag ttcggcttgg agcaacgcga gaaaaggagt cgaaaagatg
     2401 gatatccagc aactcgtttc ggagctaaaa gagaaacgcg gtgggcgaa
//
LOCUS       DAOPTR010000619         2447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_34142_length_2447_cov_4.021321, whole genome shotgun sequence.
ACCESSION   DAOPTR010000619 DAOPTR010000000
VERSION     DAOPTR010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34142_length_2447_cov_4.021321"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            306..1520
                     /gene="ribB"
                     /locus_tag="PKI05_11340"
     CDS             306..1520
                     /gene="ribB"
                     /locus_tag="PKI05_11340"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HOA52839.1"
                     /translation="MSGVFSTIEAAIEAILEGKVIIVVDDENRENEGDFICAAEKITP
                     EIVNFMITYGRGILCAAILPDVAERLDLPLIVKENTSPLGTAFTVPVDHRSTRTGITA
                     MERATTIRALIDPTSKPGDFCRPGHVYPLIAKEGGVLRRAGHTEAAVDLARLAGLAPA
                     GVLCEILNDKGDRATRSELYELAKRFNLEMITVEELIRYRRRREKLVYRIAEAQLPTR
                     YGAGRIIAYGVKFENQEPIAVVIGDPTRVPAPLVRLHSSCFTGDLIASLRCDCGDQLH
                     LAMEMIGREGAGVLVYLPQEGRGIGLVEKIKAYHLQDQGMDTVEANLALGHQADCRDY
                     GIGIQILKDLGLSKVRLLTNNPKKTDAFIYGGFDLEVVDQIPIIGPINEYNARYLATK
                     RDKMGHLLPDTF"
     gene            1900..>2447
                     /locus_tag="PKI05_11345"
     CDS             1900..>2447
                     /locus_tag="PKI05_11345"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOA52840.1"
                     /translation="MSAKVFRYAVWGLVTLVVAFGGLSVAGSAEEGKKIYILTDLEGA
                     SGVYKFAQTRESGPLNDQAKEYLMGDIAAVVRGLREGGATEIIVLDGHGSQAFVPHLM
                     VPGAKYITGIPRPSVMPYLDESFAGIVQLGAHAMMGTQDGVLCHTQSSRNENRYWYNG
                     VESGEIAQVALYAGAFGVPTIMV"
BASE COUNT          514 a          616 c          745 g          572 t
ORIGIN      
        1 ggtgagggga gatagtggga taagctcttg gttgcagcgt cgcgctactg aagctggcag
       61 aggtcttttg ggccttctag ggtggttgtt cacaaaaaag ctaaggcctc ttgacaactg
      121 gaagcattat tagcggctgg cttagaataa agtgatggct ctgatgtcgt taactgggtt
      181 gctctccggc atacggaggg gattatcccc attgatcggg gccagtagga aatcgacccg
      241 ggggctcgag cgaggcgtaa tgacaagagg ttccctcttt attcctggtt aagtaggatt
      301 gccatatgtc gggcgtattt tccactatcg aggcggctat tgaagccatt ctcgaaggca
      361 aggttatcat tgtcgttgac gatgaaaacc gggagaacga gggtgatttt atttgcgccg
      421 cggaaaagat tacaccggaa atcgtcaact tcatgattac ctacggccgg gggattctct
      481 gcgccgcaat tttgcccgac gtggccgaga gattggattt gcccttgatc gtgaaggaga
      541 acacctctcc attgggcacc gccttcactg tcccggtgga tcatcgttcg actcggacgg
      601 ggataacagc gatggagagg gcgacgacga tccgggcctt gatcgatccg acgagcaaac
      661 cgggcgattt ttgccgtccc ggccatgtct atcctctgat tgccaaggaa gggggcgtgc
      721 tccgtcgggc gggccacacc gaggctgcgg tcgatttggc ccgactggcg ggactggcac
      781 ccgccggggt gctctgcgag attctcaacg ataaaggaga tcgcgcgact cggtcggagt
      841 tgtacgaact ggcgaagcgt ttcaatttag agatgattac cgtcgaggag ttgatccgct
      901 atcgccgtcg gcgcgaaaag ctcgtctatc gcatcgccga agcacagttg ccgactcgat
      961 atggagccgg ccgaatcatc gcttacgggg tcaaattcga aaaccaagag ccaattgccg
     1021 ttgtcattgg tgacccgaca cgggtcccgg ctccgcttgt gcgtctccat tcttcctgtt
     1081 ttaccggcga cttgattgca tcacttcgct gcgattgtgg cgatcagctt cacctggcca
     1141 tggagatgat cggtcgagag ggggcgggcg tcctcgttta tttgcctcag gaaggccgcg
     1201 gcattggttt ggtggagaag atcaaggcct atcacctcca agatcaggga atggataccg
     1261 ttgaagcgaa cttggctttg ggtcaccagg ccgattgccg ggactatgga atcggtatcc
     1321 aaatcctaaa agatttgggc ctttccaaag tgcgtctttt gacgaataac cccaagaaga
     1381 cggacgcttt catctacggc ggattcgacc ttgaagtggt ggaccaaatt cccattattg
     1441 gtcccatcaa tgaatacaac gcccggtatc tagcgaccaa gcgagacaag atgggccacc
     1501 tcttgccgga taccttttga gacgtggtct tttcatcacg gctaacttgg agaaaagcgg
     1561 gcacctgctg tttggaaccc gcgagccgcc agcgtaagtg gtcggcgagt ttgtcggcca
     1621 cgacggtcag tagtcctcca tcacttcatc tgagcgatgc ctcgagaatt cccactcaga
     1681 cggctcggca atcctcgata actctagctg tggaggaacg cgcgatgtcg cgcctgtgat
     1741 cggtttaggc agtggttgct gaaagctgtc gcgccatctc ttaacgccaa gcccccattg
     1801 cctttcgttg ccaaagacaa tccctggtgg tacgatatgg ccggttggaa tcgcgagctt
     1861 ttgagttgcc tgtttccctg acgcggagga ggtgtcacga tgtcggcgaa ggtgtttcgt
     1921 tatgctgttt gggggttggt cacgttagtc gtggcttttg ggggattgag tgtcgcgggc
     1981 tctgccgagg aagggaaaaa aatctacatc ctcaccgatc ttgaaggggc tagcggtgtg
     2041 tacaagttcg cccaaacccg ggagtcagga ccgcttaacg accaggccaa agagtacttg
     2101 atgggcgaca ttgccgccgt ggtacggggg ctccgcgagg gaggggccac ggaaatcatc
     2161 gtcctcgatg gtcatggaag ccaggcattt gtgccgcatc tgatggttcc cggggccaaa
     2221 tacatcaccg gcattccgcg accttccgtc atgccctatc tggatgagag ctttgcgggg
     2281 attgttcagt tgggcgctca tgccatgatg ggcacacagg acggcgtatt gtgccacacc
     2341 cagtcatcgc ggaatgaaaa ccgttactgg tataacggcg tcgaatcggg ggagattgcc
     2401 caggtggcgc tgtatgcagg cgccttcgga gtgccgacga tcatggt
//
LOCUS       DAOPTR010000620         2447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_34143_length_2447_cov_3.686455, whole genome shotgun sequence.
ACCESSION   DAOPTR010000620 DAOPTR010000000
VERSION     DAOPTR010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34143_length_2447_cov_3.686455"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PKI05_11350"
     CDS             <1..204
                     /locus_tag="PKI05_11350"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOA52841.1"
                     /translation="DHEIGMVVDRVLGEEDVVIKSIAENYRNIPGIAGASILGDGRVS
                     LILDVAAIMGMITRRVGSHAMSS"
     gene            305..931
                     /locus_tag="PKI05_11355"
     CDS             305..931
                     /locus_tag="PKI05_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HOA52842.1"
                     /translation="MNPTTIHRVEVLRPLFAAATHEASAAMCRWTDGVISLTLDDICE
                     LPLEEVTAELGFSDDFMTMVVLGIESEPGTVLILTFDEENARQLAATLTRRQRNTTPE
                     WDELEISALTETGNILGCAYVNALTRLTGQILIPSPPYFVRDYFASVLEQALVEQAMS
                     SDTALLCRTGFHREGENLNWHVLFIPSKSLREKLERSVASVSHDRARR"
     gene            1024..1521
                     /locus_tag="PKI05_11360"
     CDS             1024..1521
                     /locus_tag="PKI05_11360"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOA52843.1"
                     /translation="MTVTASSSSLGSKIINVGMGQTAVGDKEAVFTSVLGSCIGIVLW
                     HPHLGMGAIAHVVLPDSHGQNGQPGKFADTAVPYLIRQLADIGCPRTGLVAKIAGGAC
                     MFRGGGPLQIGDDNIAATRTILRQHGIAITAEDVGGTSGRKITFECATGRLVVEVAGQ
                     TVRVL"
     gene            complement(1542..>2447)
                     /locus_tag="PKI05_11365"
     CDS             complement(1542..>2447)
                     /locus_tag="PKI05_11365"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HOA52844.1"
                     /translation="PLPIPEDNPMTVEKIELGKMLYFDKRVSADGTVSCATCHDPKMA
                     WAEHLPTSKGIHEQVGERNAPTVINAAYAPSQFWDGRAKTLEEQAVGPVANPIEMGHT
                     LEGMVQSLDNIPQYQELFKKVFGTGVTAENFAKAVAAFERTILSGNSPYDRYKAGDQN
                     ALTEAQKRGLKIFEESGCADCHAPPLFSTYEFYNAGIGMNKEKPDEGRKSVTGKEEDM
                     GAFRVPSLREVANTAPYFHDGSVATLEEAVAIMAKGGIDNPHRSAEFDTVREANITAE
                     QQKDLVEFLKALSGEFPVIEPPQLP"
BASE COUNT          533 a          676 c          652 g          586 t
ORIGIN      
        1 gatcacgaaa ttggcatggt ggttgaccgg gtactgggag aagaggacgt ggtgatcaag
       61 tccatcgcgg aaaattatcg caacataccg ggaattgccg gggcgagtat cctcggcgac
      121 gggcgcgttt ctcttattct ggatgttgct gccatcatgg ggatgatcac acgccgtgtg
      181 ggaagtcacg cgatgtcgtc ataagccaaa tgatgacttt ttaggaagta actcacaaaa
      241 cgttgccaat tgtgaccaac gtgattgatt caccagtaac catgatcgtg aaggaagtgc
      301 gtctatgaat ccaacgacaa tccaccgcgt tgaggtcttg cgacctctgt ttgctgctgc
      361 cacgcatgag gcatcggctg ctatgtgtcg atggaccgac ggcgttattt cattaacgct
      421 cgatgacatc tgcgaattgc cactggagga ggttacagca gaactggggt tttccgatga
      481 cttcatgacc atggtcgtcc tggggattga atctgaacct ggaacggtcc taatcctcac
      541 attcgatgaa gagaatgccc gtcaattggc ggccaccctc acacgtcggc aacgaaacac
      601 cactcctgag tgggacgaac tggaaatctc ggccctgacg gagacgggga atattttggg
      661 ttgcgcctat gtcaacgccc tcacacggtt aaccggtcag attctgatcc cttcaccgcc
      721 gtatttcgtt cgggattatt tcgcaagtgt tcttgagcag gcacttgtgg aacaggccat
      781 gtcgtccgac acggcccttc tttgccgcac cgggtttcac cgcgaaggtg aaaacctcaa
      841 ctggcacgtg ctgtttattc catccaagag cctgcgtgag aaattggaac gcagtgtcgc
      901 atcagtgtca cacgatcgcg ctcggaggta atctgcgtca aaggcctgat ttgcgaaggg
      961 tggtcttaca aactgtggat cacattcccc ttggtacgca ccaattaaga taggtaacaa
     1021 cgtatgactg ttacagcatc gagttcatcg ctcggaagca aaataatcaa tgtgggaatg
     1081 ggccaaacgg cggtaggcga taaagaagcc gtgttcacct ccgtgttggg ctcgtgcata
     1141 gggattgtct tgtggcatcc ccacctgggc atgggcgcaa tagcccacgt ggtccttccc
     1201 gactctcacg gccaaaatgg tcaaccagga aaattcgccg acaccgcagt accctatctc
     1261 atccgccagc ttgccgacat agggtgtccc cggactggct tggtcgccaa aattgccggc
     1321 ggtgcctgca tgtttcgcgg gggcggaccc cttcaaattg gtgatgacaa catcgcggcc
     1381 acgcgaacca tcctgcggca gcacgggatt gccattaccg ccgaagacgt cggcggcaca
     1441 tcgggacgca agatcacgtt cgaatgtgcc accggacgcc tcgtcgtgga agtcgcaggc
     1501 caaaccgtcc gtgtgcttta gtcctcacaa ttcccctctt ttcatggcaa ttgtgggggc
     1561 tcgatcaccg ggaactcgcc cgagagtgcc tttaagaact ccacaagatc cttttgctgt
     1621 tctgccgtga tattggcttc acgaacagta tcgaactcgg ccgaacggtg tggattatcg
     1681 ataccgcctt tcgccatgat ggccacagcc tcctccaacg ttgctacgct tccgtcgtgg
     1741 aaatacggcg ccgtgttggc gacctcacga aggctgggca cgcgaaaagc tcccatatcc
     1801 tcctccttgc cagtgacgct ctttcggccc tcgtcaggct tttccttgtt cattccaatt
     1861 ccggcgttgt aaaactcata ggtgctaaac agcggtgggg cgtgacaatc agcgcagccc
     1921 gattcctcga aaatcttcaa cccacgcttt tgtgcctcgg tcaacgcatt ctggtcgcct
     1981 gctttgtaac gatcataagg tgaattgcca ctcagaatcg tccgttcaaa ggcggcgacg
     2041 gccttggcga agttctcggc agtgacaccc gttccaaaga ccttcttgaa aagctcttga
     2101 tattgcggaa tgttatccaa gctctggacc attccctcga gggtgtgacc catttcgatg
     2161 ggattggcca ccggtccaac ggcctgttct tccagcgttt tagcgcggcc atcccagaat
     2221 tgcgacgggg cataggctgc gttaatcacc gtgggggcat ttcgttcacc cacttgctca
     2281 tgaatccctt tgcttgtggg caaatgctct gcccaagcca tcttgggatc gtggcacgtc
     2341 gcacaagaga ccgtcccatc ggcacttact cgcttgtcga aatagagcat ttttcccaac
     2401 tcgatctttt ctacggtcat cgggttatcc tctgggatgg gcagggg
//
LOCUS       DAOPTR010000621         2441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_34242_length_2441_cov_4.204526, whole genome shotgun sequence.
ACCESSION   DAOPTR010000621 DAOPTR010000000
VERSION     DAOPTR010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34242_length_2441_cov_4.204526"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1442)
                     /locus_tag="PKI05_11370"
     CDS             complement(<1..1442)
                     /locus_tag="PKI05_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52845.1"
                     /translation="MNRFGTCFLAVVTTFGLCGWAFAESSPAVREIVLEEIAYTDEQA
                     AQQAWRASEGTPPIRLIKVDGGQALEIGLPFARHADLPRSIHDREVKLDLSRPGEFIL
                     ELEIDRPEAVGHLSLYFRSGAGWYAGSGSVSSVGKQLVRFVKAEFRSEGKPAGWGQID
                     GIRISFWRGASVDAQARIYRLTALEHPVALLVPTAVEGHEQSELRTIREAAERVGRLI
                     TDLGLGFDTLDDRNPSAEVWAEKAIIIVPYAPRLSPTAASALAEAIRRGTKVILFYQA
                     PQEVLTLLGVGKTRWMAREYESQFAEVRFDRNILPDLPVRMRQSSWNITEPIPTVAST
                     RVIGEWFDAQGKPTGRAAMILSPHGAFFSHILLGGDAENQRVMLAAVMGALRPDLKPV
                     ILRGLLAKVTQVGHLQNLDELREWLKKYAPDTTSAPWQLLQEAESLLSQIDVKDATRP
                     EQISLAALEDIRKKIVTAYLMAQPSPSREGR"
     gene            complement(1525..>2441)
                     /locus_tag="PKI05_11375"
     CDS             complement(1525..>2441)
                     /locus_tag="PKI05_11375"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOA52846.1"
                     /translation="VTDDTEALQRAIESHPALFIPKGEYRVTRTLRLRPDSQLIGAHR
                     CFTWLVGYGDAGGDFARADQPKPIVQTANDPNARTILGFFGIRLDISGVGGYALDWAA
                     GGQSVYRDVNVVMPTRWNVPKGAAAPQFDHPFVLIHGHGAGKWYNFHQESWQLQGPHY
                     RHLLIEGIKGPLHFYQCNAEHSRSDANMEIRRASGVFVYGLKGEYNRPIVKAIDAENF
                     AIFGYGGNAAAYPGTALFDIENCRNYRLTLLVDSPRYPGSGSPDHFAGEGVDPRLWHM
                     VTEKQGDQVFRTPPLDRPTLVARGDLLR"
BASE COUNT          542 a          742 c          648 g          509 t
ORIGIN      
        1 cgaccttctc gagagggact tggctgcgcc atgagatacg ccgtcacaat cttcttgcgt
       61 atatcctcga gggctgccaa agaaatctgc tccggacgag tcgcatcctt aacatcgatt
      121 tgcgagagga gcgactcagc ctcctgaagc aactgccaag gggcgctcgt tgtgtccgga
      181 gcgtatttct tgagccactc ccgcagttca tcgaggtttt gcaagtgacc gacctgggtg
      241 actttggcca gcaggccacg cagaatgaca ggcttgagat ccggccttaa cgcccccatc
      301 acggcggcca acatcacgcg ctgattttca gcatcacctc ccagcaagat gtggctgaaa
      361 aacgctccat ggggcgagag gatcatggcg gcccgaccgg tgggcttacc ttgtgcatcg
      421 aaccactcgc cgatcacacg ggtgcttgca accgtgggaa taggctcggt gatattccac
      481 gatgactgcc gcatccggac aggcaaatcg ggcaagatat tgcgatcgaa acggacctcc
      541 gcaaattggg attcgtattc gcgggccatc cagcgggttt tacccacccc aaggagagtc
      601 aacacctctt gtggcgcttg atagaacaag atgacttttg tgccgcgacg aattgcttct
      661 gctaaggccg atgcggcagt gggcgataaa cgtggggcat agggcacgat gatgatggcc
      721 ttttcggccc aaacctcggc gcttgggttt cgatcgtcga gtgtatcaaa gcccaatccc
      781 aggtcggtga ttaatcgccc aacacgctcg gcagcttccc ggatggtccg caattcggat
      841 tgctcgtgtc cctcgacggc cgtgggaaca agtagggcga cggggtgctc cagggcggtc
      901 agacggtaaa ttctcgcttg ggcatccacg gaggcacctc gccaaaatga gatgcgaatc
      961 ccatcgatct gcccccaccc agcgggtttt ccttcagagc gaaattccgc cttcacaaac
     1021 cgtaccaact gcttgcccac gctgctgacc gatcccgaac cggcatacca ccccgcacca
     1081 ctgcgaaaat agagcgatag atgtcccacg gcttccgggc ggtcgatttc cagttccaaa
     1141 ataaactcgc cggggcggga aaggtcgagc ttcacctcgc ggtcatgaat actgcggggc
     1201 aaatccgcgt gacgggcaaa cggcaagcca atttctaagg cctggcctcc gtccacctta
     1261 atcaaccgga ttggcggcgt cccttcagag gctcgccacg cttgctgggc agcttgctca
     1321 tcggtgtatg cgatctcttc caggactatt tctcgcaccg ctggcgaact ttccgcaaac
     1381 gcccatccgc ataatccaaa ggttgtgaca acggccaaaa agcacgtacc aaaccgattc
     1441 atcgccgaaa ctccttgtgg ttcgtttccc atcactttga tcgttcttag tcctatctcg
     1501 caacattccc tatctttgca atgcctaacg aaggaggtct ccgcgagcca ccaaggtcgg
     1561 ccgatcaagc ggcggcgtgc gaaacacctg atcgccctgt ttttctgtca ccatgtgcca
     1621 gagccgagga tcaacaccct ccccggcaaa gtggtccggc gagccactcc ccggatagcg
     1681 tgggctatcc acaaggagtg tcagtcggta attacggcag ttctcaatgt caaacagggc
     1741 agtgcccgga taggccgccg cattaccccc gtatccaaag atggcaaagt tctccgcatc
     1801 aatggccttc acaatggggc gattgtactc ccccttaaga ccatacacaa aaacaccgga
     1861 ggccctgcgg atttccatat tggcatcact tcgggaatgt tctgcgttgc actggtaaaa
     1921 gtgcaatggc ccctttatcc cctcaatcaa caggtgccga taatgaggcc cttgaagctg
     1981 ccaagactcc tgatggaagt tgtaccattt gccggcccca tgtccgtgaa tcagaacaaa
     2041 cgggtgatca aactgtggtg ccgctgctcc cttcgggacg ttccagcgag tcggcatcac
     2101 cacgttgaca tcgcgataaa ctgattggcc tccggccgcc cagtcaaggg catatccccc
     2161 cacgcccgag atgtccagcc ggatcccaaa gaaaccgagg atcgtcctcg cgttggggtc
     2221 gttagcggtt tggacaatcg gctttggctg atccgcgcgg gcaaaatctc ccccggcgtc
     2281 tccgtatccc acgagccatg taaagcaacg atgcgcgccg atcaattggc tgtcaggccg
     2341 caaacgcaaa gttctcgtaa cgcggtactc gcccttagga ataaagaggg caggatggct
     2401 ctcaatggcc ctttgcaggg cctcggtatc atcggtcact c
//
LOCUS       DAOPTR010000622         2440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_34260_length_2440_cov_3.257023, whole genome shotgun sequence.
ACCESSION   DAOPTR010000622 DAOPTR010000000
VERSION     DAOPTR010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34260_length_2440_cov_3.257023"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PKI05_11380"
     CDS             complement(<1..500)
                     /locus_tag="PKI05_11380"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOA52847.1"
                     /translation="MQFPWSLIKAMTWHALKCRLTGRRRAPIVLMLEPLFACNLRCEG
                     CGRIREYHHVMDQRLSVEECLASAQECDAPIVSICGGEPLLYPELDRLVNGLIAQGRH
                     IYLCTNALLVDRWIDRLPRSSRLTWNIHLDGMEATHDAITQRPGTFQTVIQNIRRLKA
                     DGRRVTT"
     gene            complement(530..>2440)
                     /locus_tag="PKI05_11385"
     CDS             complement(530..>2440)
                     /locus_tag="PKI05_11385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOA52848.1"
                     /translation="QSEKGGWSLYPGGPIDVSASVKAYFALKLAGYSPSDPRLRRAAE
                     AILEHGGAQAVNSFTRFYLAFLGQIPYHICPAVPPEVVLLPKWFPINLYKMSAWSRAM
                     VVPLSVLWAMKPVRTLPPEKGIRELFLTRPEDWPLTRGSGAHGGCDLWSLFFQMVDRC
                     LKYAECWGFLPLRKRALRAAERWMLEHMEASHGLAAIYPPMVWSLIALHALGYSQDSP
                     VMREAWRRVEELLIDTEDQTSARVQPCLSPVWDTALTLRALMSAGLSTDDGQVQKAVA
                     WLLDHQILTPGDWSHTVNVPPGGWCFEHANPFYPDCDDTAAVLTVLATQCNHDEKQNS
                     QLFPPQWRLVDEDRRKTPQTGQRSKSRGMSPQELEELAKRTNEAIYRGVTWLLGMQSR
                     DGGWAAFDRDNCCAILTKVPFADHNAMIDPSTPDITGRVLETLGTLGYRVGFPAVDRA
                     VAFLRRTQEPDGSWFGRWGVNYIYGTWLAITGLRAVGVPEDDPAVVAGANWLLSHQLP
                     DGGWGESPATYEHPHLRGDGPSTPSQTAWAILGLLAAGKASDPALVRGIRYLLRHQLP
                     DGSWPEEAFTGTGFPRVFYLRYHMYPLYFPLLALARFATMNADRLAELDLPELHVVAP
                     PIDVEDVTARIA"
BASE COUNT          536 a          715 c          724 g          465 t
ORIGIN      
        1 gtggttaccc gccggccatc cgctttgagt cttcggatat tttggatcac cgtttggaat
       61 gtccctggtc gttgggtgat ggcgtcatgc gtcgcttcca tgccatcaag atggatgttc
      121 catgtcagcc gcgatgatcg tggcaggcgg tcgatccagc ggtcaacaag cagggcattt
      181 gtgcacaggt aaatatggcg tccctgggcg atcaaaccat tgacgagacg gtcaagctcg
      241 ggatagagca gcggttcgcc gccgcagatg ctgacgatgg gagcgtcaca ctcctgggcc
      301 gaggcgaggc attcctccac ggacagtcgc tgatccatga catgatgata ttcccgaatt
      361 cggccacagc cctcgcatcg caagttgcaa gcaaaaagtg gttctagcat gaggacaatg
      421 ggcgcacggc gacggccggt caaccggcac ttcagcgcat gccaggtcat ggccttgatc
      481 aacgaccagg gaaactgcat cgcgctcgac tccttcaagc gatcatggat taggcaatgc
      541 gggccgtcac atcctcaaca tcgattggtg gtgccaccac gtgcaactcc ggcaggtcaa
      601 gctcggcaag tcggtcggcg ttcatggtgg caaaacgagc cagggccaaa agcgggaaat
      661 agaggggata catgtggtag cggagataaa acacgcgggg gaagcccgtc ccggtgaagg
      721 cctcttccgg ccaggaacca tccggcaact ggtgccgcaa caaatatcgg atgccgcgga
      781 ccagcgcggg gtcactggcc ttccctgccg ccaaaagtcc caagatagcc caggctgttt
      841 gcgacggtgt actggggccg tcaccgcgta ggtgaggatg ctcataggta gccggtgatt
      901 caccccaacc cccgtcgggc aactgatggc tgaggagcca gtttgccccg gcgacgacgg
      961 cgggatcgtc ttccggaacg cccaccgcac gtaacccagt gatggccagc caggtcccat
     1021 agatgtagtt gaccccccag cgaccgaacc aactgccatc gggctcctgg gtccgcctga
     1081 ggaaggccac cgcccgatct actgcgggaa accccacgcg ataaccgagt gtccccaatg
     1141 tctccaacac tcgccctgtg atatcgggag tgctcggatc gatcatcgcg ttgtggtcgg
     1201 caaaggggac tttggtgagg atcgcgcagc agttgtcccg atcaaatgcg gcccaaccgc
     1261 catcgcggct ttgcatcccc agtaaccatg taacgccgcg atagatcgcc tcattagtcc
     1321 gcttggcaag ctcttccaat tcctgcggcg acattccgcg actcttggat cgttgccccg
     1381 tttggggtgt cttgcgtcta tcctcatcaa cgagccgcca ttgtgggggg aataattggg
     1441 agttctgttt ctcgtcgtga ttgcactgcg tggccaagac tgtgagaacg gcagccgtgt
     1501 catcacagtc ggggtagaaa gggttggcat gttcaaaaca ccatccgcct ggcgggacgt
     1561 tcacggtgtg agaccaatca cctggcgtca gaatctgatg atcgaggagc cacgccacgg
     1621 ccttttggac ctgcccatcg tctgtggata ggccggcaga catgagagcc cgcagggtca
     1681 gggcagtatc ccaaacaggg gagagacacg gctgaacccg ggcactggtt tggtcttcgg
     1741 tgtcgatgag caattcttcc acgcggcgcc aggcctcccg catgacgggc gaatcctgcg
     1801 aataccccag ggcgtgcagg gcaatcaaac tccagaccat cggcggatag atggccgcca
     1861 gtccgtgact ggcctccatg tgttccaaca tccacctctc tgccgcgcgc aaagcacgct
     1921 tgcgcaaggg gaggaatccc cagcattcgg catacttcag acagcgatcc accatctgga
     1981 agaataagga ccaaagatcg cagcccccat gtgcccctga accccgggtc aatggccagt
     2041 cttccggtcg agtcaaaaag agttcgcgga ttcctttctc aggcggtaac gtgcgaacgg
     2101 gcttcatcgc ccacaacacg gataaaggaa ccaccatcgc ccgtgaccaa gcgctcatct
     2161 tataaaggtt gatgggaaac cacttcggaa ggaggacgac ctcgggaggc accgccgggc
     2221 aaatgtggta aggtatttgt cctaaaaagg ccaggtaaaa acgagtaaaa ctgttgacgg
     2281 cctgagcgcc cccgtgctca agaatggcct ctgcggcgcg acgcaatcgt ggatcactgg
     2341 gggaatatcc agccagtttg agggcgaaat aggccttgac gctggcgctt acgtcgatcg
     2401 gaccaccggg atagagcgac cagccgcctt tttctgattg
//
LOCUS       DAOPTR010000623         2431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_34441_length_2431_cov_4.446970, whole genome shotgun sequence.
ACCESSION   DAOPTR010000623 DAOPTR010000000
VERSION     DAOPTR010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34441_length_2431_cov_4.446970"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..1149)
                     /locus_tag="PKI05_11390"
     CDS             complement(139..1149)
                     /locus_tag="PKI05_11390"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA52849.1"
                     /translation="MANWRGIFRRWEPQDIAPVVSLAALIVFFSIAAPGFFRWNTLVT
                     AMQQGAVLAIVSAGLTFVLLAAEIDLAVGAVALWTACCCGWLFQNWATQTTEGRFVAS
                     PGFVATVIVLPLLTSLLLGTLSGFLTVQSRLPSFIISLAMMFISQGAARYITRGQAFR
                     VPELLNYVGNRGLEHEGLTVVPYSAMLAAVIMLVGHIVLQYTRFGRYVYMTGGNRQAA
                     RLAGIPTGVIVTACLAISAVTAGIGGLVNAGRMTSVTLDQNQDLLLSALACIVLGGTS
                     LFGGEGSMGKTLVGVATFTVLNIGLNSIRLDDFARPFLMGVILLASLLLNAILSRRQQ
                     WS"
     gene            complement(1250..>2431)
                     /locus_tag="PKI05_11395"
     CDS             complement(1250..>2431)
                     /locus_tag="PKI05_11395"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOA52850.1"
                     /translation="RQPLTKWGIVDWRAMVSWAEEYLHDLGIKLDVSRRLDRYPLVVR
                     QMVEIIKGAYRGARVLILDEPTSALSPPEIERLFELIRRLKSRGVAMIFISHFLEDTL
                     RISDRVTVLREGRRVLTRLCHEVTKQDLVRAMVGERCEEMAMADQQVVTLPPPSQNPP
                     VLAAEDLTLRGVFEGIRLQVAPGEILGLYGFVGAGHQELVQAVAGAQLPTRGRILLDG
                     QPLPLGKPRAAVRKGVVLVTADRAAGLFMRGEVYKNVTMAYLEQAVGEWITARREVMA
                     VQPVLERVACSPPDPWMKAGQLSGGNQQKVVFAKWLLGPVRVLLLEEPTRGMDIAAKE
                     DVMRLVQDLAKQGVAVILASTEPELILRYAHRIIVFSRGRITASWAGETVTKQDLMRA
                     A"
BASE COUNT          572 a          740 c          664 g          455 t
ORIGIN      
        1 gaaaaccggc agcgattcga gctaccctca cccccgaccc ctctcccgcc gaacgggcga
       61 ggacagatta cgggataggc tcttatccat tcgcattgtc actgatgcct ggtccgtgaa
      121 caatacgagg ccttttcatc acgaccactg ctgtctgcga gagaggatcg cattcagcag
      181 cagcgacgcc aaaaggatga cacccatcag gaagggacgg gcgaaatcgt ccaggcgaat
      241 cgagttcagg ccaatattga ggactgtaaa agtcgccaca cccacgagcg tcttgcccat
      301 gctaccctct ccgccgaaaa gacttgtccc gccgaggacg atgcaggcca gggcactgag
      361 gaggaggtct tggttctgat cgagtgtaac gctggtcata cggccggcgt tcaccaagcc
      421 cccgatcccg gccgttaccg cagagatggc caggcaagct gtcacaataa cgcctgtcgg
      481 aatacctgcc aatcgagcgg cttgccgatt tccaccagtc atgtaaacat accggccaaa
      541 tcgcgtgtac tgtaacacga tatggccgac caacatgatc actgccgcca acatggcact
      601 gtaggggaca acagtcagac cttcgtgttc cagaccacgg tttcccacgt agttaagcag
      661 ctcggggacg cggaaggcct ggccccgcgt gatatacctt gcggcccctt gggaaatgaa
      721 catcatggcc agggagatga tgaaacttgg cagccgggat tgcacggtga gaaaccctga
      781 cagcgttccc aaaagcaagc tggttaagag cggcaagaca atcaccgtgg cgacaaagcc
      841 gggagaagcg acaaaacgtc cctcagtggt ttgtgttgcc caattttgaa agagccaccc
      901 acaacagcag gccgtccaaa gagccacggc gcccacagct agatcaatct cggcagcaag
      961 caagacaaag gtaaggccgg cactgacgat cgccaagacc gccccctgct gcatggccgt
     1021 cacaagcgta ttccagcgaa aaaagccggg ggcagctata gaaaaaaaaa caatgagcgc
     1081 tgctagcgaa acgacggggg caatatcctg tggttcccaa cgtcgaaaga ttcctcgcca
     1141 attggccatc gcatttcacc ctgctgaaat cttctcgaat tggtataaga tccgattata
     1201 agttgttcga tacgcgtgga ggtttccgcg ggaaggacga tccagccgac tatgctgccc
     1261 gcatcaggtc ctgtttggtg accgtctctc ctgcccaact ggcggtgatc cgcccgcggc
     1321 taaagacgat aattcggtgg gcatagcgga gaatcaactc cggctccgtt gatgccaaaa
     1381 tcacggccac gccctgtttt gccaaatcct ggactaatcg catgacatct tccttggcgg
     1441 cgatatccat gccccgggtt ggttcttcca aaagaagaac gcggactggt cccaacagcc
     1501 atttggcgaa gaccaccttc tgctggtttc cgccggagag ttggccggcc ttcatccaag
     1561 ggtctggcgg agagcaggcc accctctcca agacgggctg aacggccatg acttcacgcc
     1621 gggcggtgat ccactccccg acggcttgtt cgagataggc catcgtgacg ttcttgtaca
     1681 cctcaccacg catgaacagt ccggcggcac ggtcggccgt aaccaagacg accccctttc
     1741 gcacagccgc gcgaggttta cccaggggca acggctgtcc atcaagcaga atgcgcccac
     1801 gggtgggcag ttgcgccccg gccacggcct ggaccaattc ctgatgccct gcccccacaa
     1861 agccgtacag tcccaggatt tcacccggcg ccacttggag acgaattcct tcgaagacac
     1921 cacggagtgt caggtcttca gcggccaaca ccggtggatt ctgggatggg ggaggtaggg
     1981 tcaccacttg ttggtcggcc atggccatct cctcgcagcg ttcccccacc atggcccgta
     2041 caagatcttg cttggttacc tcgtggcaga gacgtgtgag aacacggcgg ccttcgcgca
     2101 agaccgtcac ccgatcggag atccgaaggg tatcctccaa gaaatgacta atgaagatca
     2161 tcgccactcc acgtgacttg agccgccgga tcaactcgaa cagtcgttca atttccggag
     2221 ggctgagggc acttgtcggt tcgtcaagga tcagcactct ggcgccccga tacgcccctt
     2281 tgatgatttc gaccatttgc cggacgacaa ggggatagcg atccagtcgg cgggagacat
     2341 cgagcttgat tccgagatcg tggagatatt cttcagccca cgaaaccatg gcccgccaat
     2401 ccacaatccc ccatttcgtg agcggctgcc g
//
LOCUS       DAOPTR010000624         2430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_34461_length_2430_cov_3.754105, whole genome shotgun sequence.
ACCESSION   DAOPTR010000624 DAOPTR010000000
VERSION     DAOPTR010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34461_length_2430_cov_3.754105"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..1080
                     /locus_tag="PKI05_11400"
     CDS             46..1080
                     /locus_tag="PKI05_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1593 domain-containing protein"
                     /protein_id="HOA52851.1"
                     /translation="MLRHLMIVLVAFLASGLRLVAVAETVQRPRLAVLTDIGGDPDDQ
                     QSMIRLMMYSNEFDIEALIATASGTPGELKVAVTRPDLILQIIDAYEKVLPNLRKHAQ
                     GWPEAETLRKCVKSGNPQRGLKNIGEGHDSEGSRYLIERIDAGGPELPLNITIWGGQS
                     DLAQALWRVKKERGETGLAEFVKKFRVYDIGDQDGIADWMRREFPGMYYILSKAPPGR
                     DKRESTYRGMYLTGDISLTSRQWIEEHVRSCGPLGQLYPTKTSTAPNPHGCLKEGDTP
                     SWFFFLPLSGNDPNDPSKPGWGGQYRRGDDGWYHDLPITADFDPRTTVSQWRPVFQAD
                     FAKRMRWCVE"
     gene            1401..>2430
                     /locus_tag="PKI05_11405"
     CDS             1401..>2430
                     /locus_tag="PKI05_11405"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA52852.1"
                     /translation="MSRTFRTSRRGFLKTTGAALALPYIITSSALGEGERPPASERIV
                     MGGIGIGNMGRADLGAFLRRPDVQYVACCDVRKSAREQAKATVDNHYQNNDCKAYNDF
                     RDLLARTDIDAVHVATPDHWHAIMVIEACRSGKDVYCQKPETLTLREGPLMIEAARRY
                     GRVVSGGSQRVLEDYREVVNKCWSGELGRIKWINVNVGPLSQPCYLPPEEVPDDIDWD
                     MWLGPAPWAPYNKARCDGNFSTSGGSWRSYIDYSGGGMTDWGAHHFGGATFAVDVREL
                     QPQEVIYHNEGGREFLEYRYPNGIVLYHNRPNTGNLQVEGTPGETREPKPVPGYKGTG
                     GIYGDFIEC"
BASE COUNT          535 a          625 c          758 g          512 t
ORIGIN      
        1 cgcgtagggt gatagcttcc tgaaaacaga aggagtggtt gaatcatgct gcgccatctg
       61 atgattgtgc tggtggcgtt tctcgcaagc gggttaaggc tcgtggctgt ggcggagact
      121 gttcagcggc cccgcttagc cgttcttacg gatatcggcg gagatccgga tgatcagcag
      181 tcgatgattc gattgatgat gtattcgaac gagttcgaca tcgaagcact gattgccact
      241 gcctcaggga cgccaggaga acttaaagtc gccgtcactc ggccggacct tatcctccag
      301 atcatcgacg cctacgaaaa ggttttaccc aatttgcgca aacacgctca aggctggccg
      361 gaggcggaaa cactgcgtaa gtgcgtcaaa tcaggcaatc cacaaagggg gcttaaaaac
      421 atcggagagg ggcacgacag cgagggctcc cggtatctca tcgaacgaat cgatgctggt
      481 ggcccagaac tcccgctcaa tatcaccatt tggggtggcc aaagcgatct ggcgcaggct
      541 ttgtggcggg tgaaaaagga gcgcggcgaa accggtttgg ccgaatttgt caagaagttc
      601 cgcgtgtacg acattggcga tcaggatggg atcgcggatt ggatgcggag ggagtttcct
      661 ggaatgtatt acatcctttc gaaggcaccg ccggggcgcg ataaacggga atccacctac
      721 cgaggaatgt acctgacggg cgatatttcg ttgactagcc ggcagtggat cgaggaacac
      781 gtccgatctt gtggcccatt gggtcaactt tatcccacca agacctcaac ggcccctaac
      841 cctcatggct gtctgaaaga aggggacacg ccgtcgtggt tcttcttttt gcccttgagc
      901 ggcaatgacc ctaatgaccc ttcgaagcca ggctgggggg gacagtatcg tcgtggcgac
      961 gatggatggt accatgattt gcccatcacc gctgactttg acccgcggac cacggtgagc
     1021 caatggcggc cagtcttcca ggccgatttc gccaagcgaa tgcgttggtg tgttgaataa
     1081 cggaattgtg ctttgcgttt gtctcgaccg agcataggaa tcaccgggcg aaatcggcaa
     1141 gtgcgcatgg cagagattga aatggccagc ctatgtgctg gtaaaaggct aacctcagcc
     1201 gatgctgggg gcgccgcttg accaggtgga aggcagtatc taagatgggc gctctgagag
     1261 ggctgtgggg tttgcccatg cgtggcaagg gcgaacatct tgcagactgc gatgatggag
     1321 gagagcccct catgcccggg ttagcctcgt tctctgggta agtttgttga acgtgttctt
     1381 tttttaaata ggagaacgtc atgagtcgca ccttcagaac aagccgccgg ggtttcttga
     1441 agaccaccgg ggccgcgttg gctttaccgt acatcatcac cagctcggcg ctgggcgaag
     1501 gtgagaggcc gccagcaagt gagcgtatcg tgatgggcgg aattggtatt gggaacatgg
     1561 gacgggctga cctgggggct ttcctaaggc ggccggatgt gcagtacgtt gcttgctgtg
     1621 acgttcgcaa gagcgcccgc gagcaggcca aggctacggt cgataatcac tatcaaaaca
     1681 atgattgcaa agcttataac gacttccgcg atctgcttgc ccggaccgat atagacgcgg
     1741 ttcacgtggc aacccccgac cattggcacg ccatcatggt catcgaagcc tgccgcagcg
     1801 gcaaggatgt ctattgccaa aagcccgaga cgcttacact ccgcgaaggt ccgttgatga
     1861 tcgaagccgc ccggcgatac gggcgagtcg tctccggcgg gagtcaacgg gtcttggagg
     1921 actaccggga agtggtcaat aagtgctgga gcggcgagct ggggcggatc aagtggatta
     1981 acgtcaatgt gggtccgctc tcgcagcctt gctatctgcc tccggaagag gtccccgacg
     2041 acatcgattg ggatatgtgg cttggcccgg caccctgggc cccttacaac aaggcccgtt
     2101 gcgacggcaa ctttagcacc tcaggcggaa gctggcgatc atatatcgat tactctggtg
     2161 gcggcatgac ggattggggc gcgcatcact tcggtggggc cacttttgcg gtggatgtcc
     2221 gcgaattgca gccgcaagag gtcatttacc acaacgaagg gggacgcgag ttcctcgaat
     2281 accggtaccc gaatgggatt gtgctctatc acaaccgtcc taacacgggc aatctccaag
     2341 tcgaagggac tcctggagaa acccgcgagc cgaagcctgt cccgggctac aaggggaccg
     2401 gcgggattta tggcgatttt atcgaatgcg
//
LOCUS       DAOPTR010000625         2428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_34510_length_2428_cov_3.432364, whole genome shotgun sequence.
ACCESSION   DAOPTR010000625 DAOPTR010000000
VERSION     DAOPTR010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34510_length_2428_cov_3.432364"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PKI05_11410"
     CDS             complement(<1..549)
                     /locus_tag="PKI05_11410"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOA52853.1"
                     /translation="MAGWILNLPPTGAWYNWRPGVPHRKSNLAVDVFWVILWTVISTL
                     PLQPLFAESGLRFARRVINPESEFTACAIFDVNGDGRLDIVAGGWWYEAPTWKKHFLR
                     DVPQIRGRYDDYSNLSLDVNGDGWPDVISANYRSESLFWIQHPGPGLGPWQVHLIEKP
                     GPMETGRLYDVDGNGTLDVLPNG"
     gene            881..1132
                     /locus_tag="PKI05_11415"
     CDS             881..1132
                     /locus_tag="PKI05_11415"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOA52854.1"
                     /translation="MKCDELLAALNAYIDGEIDPQVCAELSRHLEGCNPCRVVIDTLR
                     KTITLYKGEVPYELPPEVHARLHECLKKKWQEQFGQSVS"
     gene            1162..1383
                     /locus_tag="PKI05_11420"
     CDS             1162..1383
                     /locus_tag="PKI05_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52855.1"
                     /translation="MSRDLVLIIAIFAGWIILNKWILPALGIPTCMSGGCCGPSACST
                     PFEESSPATQERAPDEGRSVSPPIEDEPK"
     gene            1408..1641
                     /locus_tag="PKI05_11425"
     CDS             1408..1641
                     /locus_tag="PKI05_11425"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOA52856.1"
                     /translation="MTIERGLRLMAGVVVTLSAALAYFHSPLWLILTAFVGLNLIQSA
                     FTNWCPAVWMLSKLGLRACCECEQTNSVASPTG"
     gene            1820..>2428
                     /locus_tag="PKI05_11430"
     CDS             1820..>2428
                     /locus_tag="PKI05_11430"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOA52857.1"
                     /translation="MHYPWWYVPVLTSPMLIAVIAVLHVLVAHYAVGGGLFFAVETSF
                     AYRTGNREYLDYLRRHAPFFILLTVVFGAITGVGIWWTIGLASPLATEVLIRTFVFGW
                     ATEWVFFVIELTAAFIFYYYWGRLAPRTHQIVAWIYAAAAWISLVLITGITAFMLSPG
                     RWLESPSFWTGFFNPQFLPQVISRTGGAILLTSLYVYLHSSLL"
BASE COUNT          505 a          689 c          640 g          594 t
ORIGIN      
        1 cccgttgggc aaaacatcca gcgtgccgtt tccatccaca tcgtaaagac ggccggtctc
       61 cataggaccg ggtttttcga tgagatgaac ctgccaaggc ccgagccccg gtccagggtg
      121 ttgaatccag aagaggcttt cgctgcggta gttcgcgctg ataacgtcgg gccaaccatc
      181 cccgtttacg tcgagggaaa gattggaata atcgtcatag cgaccccgaa tttggggaac
      241 gtcgcggagg aaatgctttt tccaggtcgg cgcttcgtac caccacccgc cagccacgat
      301 atccaaccgg ccatcgccgt taacgtcaaa tatggcacag gccgtaaact ccgactctgg
      361 attgataacc cgtctggcga atcgcaagcc gctctccgcg aacagtggtt gcagcggcaa
      421 ggtgcttatc accgtccata aaatcaccca aaaaacatcg acagcaagat tcgacttccg
      481 atgcggaaca cccggacgcc agttgtacca cgcaccggtg ggtgggagat taagtatcca
      541 gccagccata tcgtaaccct catgtcagac agcagaatca agaaagcagc gggctcgggt
      601 taacgctgcc cattgtactt atctggcgcg tcaccaaaag aggctagatg ccacggactt
      661 tggtctcgga ctattgacac aacgcgcttt gccccattct ctgtgccggc gagcgtaagc
      721 cggcagctta ggcgttgttg gcgagattcg ccccctgcgg cgccgggttc acctggtacg
      781 gcgattcttc ggcaacatgt tttccgttaa acttgtaact ttgcccgatg cggagcatca
      841 aaccgagtag tagcaaatac cttgggcgag gttacggtca atgaaatgcg atgaactgct
      901 ggccgctcta aatgcttata tcgacggtga gatcgacccc caagtatgcg cggagctttc
      961 ccgccatctt gaggggtgca acccctgtcg agttgtcatc gacacccttc gcaaaacgat
     1021 cacgttatat aaaggtgaag tcccctatga acttccgccg gaagtccacg ccagactaca
     1081 cgaatgtctg aagaaaaaat ggcaagagca gttcggccag tctgtgtcct aacgcagtaa
     1141 aaactcgaga aggaagcaaa aatgagtcgc gatttggtct tgattattgc catttttgct
     1201 ggttggatta ttcttaataa gtggattctc ccggccctcg gcattccaac ttgcatgagc
     1261 gggggttgtt gcggtccatc ggcatgttcc acaccgtttg aggaatcttc tccagctacc
     1321 caagaacgtg ctcccgacga ggggcgatcg gtaagtccac cgattgaaga cgaacctaag
     1381 tgatgcgttg aaaggaggtt acggaatatg accatcgaac gtggactacg cttaatggcg
     1441 ggtgtcgtcg tcacattgag tgcggcgcta gcctattttc attctcctct ttggctaatt
     1501 ctgacggcct tcgtgggcct caacctaatc cagtcggcct ttaccaactg gtgcccggcc
     1561 gtttggatgc ttagcaaact gggactccgg gcgtgctgcg agtgcgagca gacgaactcg
     1621 gtggcgtcgc cgacagggtg agccccgcaa ttcggcaggg caccgcccgg cggtgcctcc
     1681 acgaaatttc cctctgccct cgcttagtat gcaagggctt gcttaacgtc gccccagcag
     1741 caacaatatt ttttactgtt tcgtctgttg ttttacgggt tttccactct ggcgccagcg
     1801 gttttgggaa acataaagta tgcattatcc ctggtggtat gtgccggttt tgacgtcccc
     1861 aatgctcatc gcggtgatcg cggtactcca tgtgctcgtg gcccactacg cagttggtgg
     1921 gggcttgttt ttcgccgttg agacatcgtt tgcttaccgc accggcaacc gcgagtacct
     1981 ggactacctc cgccgacatg cccccttttt catcctcctc accgttgttt ttggcgcgat
     2041 caccggcgtg ggcatttggt ggacaatcgg attggcttcc ccgctggcca cagaagtgct
     2101 tatccggacc tttgtctttg gttgggcgac cgaatgggtt ttcttcgtca ttgaactgac
     2161 ggcagccttt atcttttact actactgggg ccgcctggca cctcggactc atcaaattgt
     2221 cgcgtggatt tatgccgctg ccgcctggat cagccttgtg ttgattacgg gcattaccgc
     2281 gttcatgcta tcccctggcc gctggctgga atctccgtcc ttctggacgg gttttttcaa
     2341 cccgcaattt ctgccccagg tcatctcccg aacaggcggg gccatcctgc tgacatcctt
     2401 gtacgtctat ctgcacagct cactcctc
//
LOCUS       DAOPTR010000626         2423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_34599_length_2423_cov_3.308699, whole genome shotgun sequence.
ACCESSION   DAOPTR010000626 DAOPTR010000000
VERSION     DAOPTR010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34599_length_2423_cov_3.308699"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PKI05_11435"
     CDS             complement(<1..653)
                     /locus_tag="PKI05_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52858.1"
                     /translation="MKTSVWIMLAFAIGLLAPPCFSQGLTEERLRQLELETQRLRAEL
                     ESLQESRPIAFPPIQVDQTGFVGAPQDAAQTPSMDEIRAEMKKIAWTKGDIQIIPYGY
                     LWATTAYESSRSQVGDYVLYVLSNTDQGESAWHVDAKSTRLGIDFVGPTLPNFGGAQT
                     GGKVEIDFQGQYQTENKGTVLLRHAYLEVKNEYFRLLAGQTWDVISPLLPGTIMYSVG
                     "
     gene            complement(1175..2014)
                     /locus_tag="PKI05_11440"
     CDS             complement(1175..2014)
                     /locus_tag="PKI05_11440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOA52859.1"
                     /translation="MPRPELSVVIPCKNEDHHLEDCVLSVVDWADEVVIADSGSTDRT
                     LEIAARLCERFPTRCRLVQREFINFGSFKSWASQQCRHDWVFVLDADERMSKSLSHEI
                     DTVLSSSSAADAYLVRFRHYFLGHRIRFGKWNGRGVIRLFRQSRCHYDSREVHEKVIV
                     PSGRVRKLHSRLDHFTCTSLDRWFQKKYAYARYGANELAKRGRRARAYDLFLRPLIQF
                     IAGYIFRLGFLDGIPGLIVAFDGAFSTYLKYVKLWEIQQNQKSSPIQQNPQILPDSKK
                     QAA"
     gene            complement(2040..>2423)
                     /locus_tag="PKI05_11445"
     CDS             complement(2040..>2423)
                     /locus_tag="PKI05_11445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOA52860.1"
                     /translation="ERATVQRIAREVNHRRVVSMADHPLDLQTLKACLARCHVMVSTD
                     SGPRHVAAAFGKPVITLFGPTSPIVTANPTVQSIDLQIPIDCAPCSCRTCPKGHHRCM
                     KDLSPELVFEHLQPFLIQQDMRGAA"
BASE COUNT          589 a          645 c          621 g          568 t
ORIGIN      
        1 ccgaccgagt acatgatcgt ccccggcaat agaggcgaga tcacgtccca agtttgcccg
       61 gcgagcaaac gaaagtactc gttcttgact tcaagatagg cgtgccgtaa aaggaccgtg
      121 cccttgtttt ccgtctgata ctgaccctga aaatcgattt ccacttttcc tcccgtctga
      181 gcacctccaa agttgggcaa cgtgggaccg acaaagtcga ttcccaaacg ggtggatttg
      241 gcgtcaacgt gccatgcaga ctcaccttgg tcggtgttcg aaagaacgta aagcacgtaa
      301 tccccgacct gtgatcgaga ggattcatag gccgtcgtgg cccaaagata cccataagga
      361 atgatctgaa tatcgccctt ggtccacgcg atttttttca tctccgcacg aatctcgtcc
      421 atactcgggg tctgcgccgc atcttgcgga gctcccacga agcccgtttg gtcaacttga
      481 atgggcggga atgcgatcgg ccgcgattct tgaagggatt cgagctccgc ccgcaaccgt
      541 tgcgtttcta actccaattg gcgcagtcgc tcctcggtca gtccctgtga aaaacacggc
      601 ggcgcaagta gcccaatggc aaaagccaac atgatccaga cacttgtttt catcacttca
      661 ctccaattcc taggagatgc agaggtttaa tggccaagag tactactgtc tagttccaca
      721 tcagcgcccg gccggggcaa ccttttgtct gaccattcgg tgctgtccag tctcttgctc
      781 attcggacag ggcactatct caggttgtcc ggccaatgga aacgtcatgc gctgttaaca
      841 ttttcagcac aagtccgaaa ggccttatcc gaaataggtc tggcaaaacc tgtataagcc
      901 tatctgctct catcgtcagc acgttaggaa tctaattaag agccatcggc aaagggttgc
      961 aaaaaagcac atcgagcctc gccaggaggt tccagatgta tcgcctttac tgagattgcg
     1021 gagaggagac cgtaagtcgc ttgggttgac gcggccgatt gccaggacat ggcaaggatt
     1081 tttgggttgt agttacggga cagcgacgcg cggcaatgct ataacgaacg tgaaagctac
     1141 cgccgagttt gccacaaggt tcaaggcaaa atggtcacgc agcttgtttc ttactgtccg
     1201 gcaggatttg tggattctgc tgaataggcg aacttttctg attttgctga atttcccaga
     1261 gcttcacgta tttgaggtat gtcgaaaagg caccatcgaa tgcgacaata agacctggta
     1321 ttccgtcaag aaagcccagt cggaaaatat aacccgcgat aaattgaatc aatggtcgta
     1381 agaacaggtc gtacgctctt gcccttcgtc cgcgctttgc cagttcattc gctccatacc
     1441 tagcgtaagc gtactttttc tggaaccagc gatcgagcga agtgcacgta aagtgatcta
     1501 accgagaatg gagtttccgc acgcggccac tgggcacgat gaccttctcg tgaacttccc
     1561 ggctatcgta atggcaccgg gattggcgaa aaagccttat gacaccccta ccgttccatt
     1621 tgccaaagcg aatccgatgt cccagaaaat agtgcctaaa ccgcacgagg taggcatccg
     1681 ctgccgacga agaggataag acggtgtcta tttcatgcga gagcgacttg ctcatccgct
     1741 catctgcgtc gaggacaaag acccaatcgt gacggcattg ttgacttgcc cagcttttga
     1801 aactaccaaa attgatgaac tcacgctgaa cgagtcggca cctcgtcgga aacctttcgc
     1861 ataacctcgc tgcaatttcc aatgtgcggt ccgtggaacc actgtcggca ataacgacct
     1921 cgtcggccca atccacaaca gacaagacac aatcttccag gtgatggtct tcgtttttgc
     1981 aggggataac gacactgagt tcgggtcgag gcatgactta cagatcctct ttctgagtat
     2041 caggctgccc cccgcatatc ctgctgaata agaaaaggtt ggaggtgctc aaacaccagc
     2101 tcgggcgaaa ggtccttcat gcagcggtga tgccccttcg gacacgttcg acagctacag
     2161 ggagcgcaat caatagggat ttgaagatcg atgctttgca cggtggggtt ggcagtgaca
     2221 atgggtgagg ttggtccaaa gagtgtgatg accggttttc caaatgccgc agccacgtgc
     2281 ctgggaccgc tgtcggttga aaccatcaca tggcaccggg ccagacatgc cttaagtgtc
     2341 tgaaggtcca gcggatgatc cgccatcgaa actactcgtc tgtgattcac ctcgcgtgca
     2401 attctttgca cggttgcgcg ttc
//
LOCUS       DAOPTR010000627         2420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_34642_length_2420_cov_3.112051, whole genome shotgun sequence.
ACCESSION   DAOPTR010000627 DAOPTR010000000
VERSION     DAOPTR010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34642_length_2420_cov_3.112051"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..1457
                     /locus_tag="PKI05_11450"
     CDS             222..1457
                     /locus_tag="PKI05_11450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOA52861.1"
                     /translation="MTPKSWWNRTILGAGITSAFGDLCYEMGNVVLPGLLSALGIPAA
                     LLGVMEGVADGLASFTKLVAGYIGDRFGHRKTLVVAGYALNTIGQGFMAIATGFWLIA
                     LGRILAWFGRGIRGPLRDEIMTAAVPPEQRGRAFGFHRAADTFGAILGPAIGTGLVAW
                     LGSSLATVSPTMPFRSVLVISMIPGALAVLSFAVLVHDPGGGGKPKLRFWGAMRSMPS
                     SFRHYLVAVGIYGSGDFAPTLLILAATQTLSPSYGLTMAGALAGSLYVLRNTLQTLVS
                     YPVGWLSDRLGARRALVGGYVLGTLSCVLLAIIFALGYWNILPLAAVFILSGICAAVQ
                     ETVEPVVTAALVPAEIRATCYGVLGAVNGVGDLISSFFVGLIWTLVAPQWAFLAAGGT
                     LLMGTALLAALKNHGADSA"
     gene            complement(1490..>2420)
                     /locus_tag="PKI05_11455"
     CDS             complement(1490..>2420)
                     /locus_tag="PKI05_11455"
                     /inference="COORDINATES: protein motif:HMM:NF027708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Sb-PDE family phosphodiesterase"
                     /protein_id="HOA52862.1"
                     /translation="GNVWPTVRVDEAYRVGLDVLALSDHIEYQPHKADVPTNHNRPFE
                     VALGRARELGIILIRGAEITRDTPPGHFNVIFLKDINPLVKDDLVEAIAAGCEQGGFV
                     FWNHHAWKGEELGRWLDIHSTLYEKKLLHGMEVANGDDYYPTAHRWCLEKGLTMVGNS
                     DIHGPDLLQKNTSAEHRTMTLVFVAEKSEQGVLEALRAGRTAVWCGPLMIGKPEWLTA
                     LAEASLEIDPPHMRSKTSVWVKVRNKCAMDLVLKRTGQFGPAEVTLKANTTAIFRINV
                     PDASQPIPLEYEITNFLAAPGEGLDVSYTIPAP"
BASE COUNT          449 a          685 c          686 g          600 t
ORIGIN      
        1 cgtcggagtt gatcgagaaa accggcagcg attcgagcca ccctcattcc gccccctttc
       61 ccgccggacg ggtgagggaa gattgtggga taggcttcaa gtggggtgat taatgagtta
      121 acctaccttg ccttcttgta gcattccgtg cttgccaact cggtgaattc tgtatagcaa
      181 cacaataggc gaatacgatc ctgtttcaag ttttcaaaat catgaccccg aaaagttggt
      241 ggaaccgaac gatcctcggt gcgggcataa ccagtgcctt tggcgatctg tgttatgaaa
      301 tgggcaatgt ggtgttgccc ggactcctat ctgccctggg aattcccgcc gctttactcg
      361 gagtgatgga aggcgtggcc gacggtctcg ccagttttac aaaactggtc gccgggtata
      421 tcggcgatcg gtttgggcat cgtaagacgc ttgttgtggc aggttatgcc ctcaatacca
      481 ttggtcaagg attcatggcc attgccaccg ggttttggct tattgccctt ggacgcatcc
      541 ttgcatggtt cggacggggt attcgtgggc cgctccgcga cgagatcatg acggcggcag
      601 tgccgccgga acagcgcggt cgggccttcg gttttcaccg cgcagccgac acatttggag
      661 cgattctggg gccagctatc gggaccggct tagtggcttg gcttggcagc tcattggcta
      721 cagtgtctcc aacaatgccg tttcgctcgg tcttggtgat tagcatgatc ccgggggccc
      781 ttgcggtttt gagtttcgcg gttcttgttc atgatcccgg gggaggtgga aaaccaaaac
      841 tgcgtttttg gggtgctatg cgctcgatgc cgtcttcctt ccgccactat cttgtagcag
      901 tgggaatcta cggaagtggt gatttcgctc ccacacttct catcctggca gccactcaaa
      961 cactgtcgcc atcatacggg ctcacgatgg cgggggcttt ggcgggctca ttatacgtac
     1021 tgcgcaacac acttcaaacg ttggtttcct accccgtcgg ctggctctcc gatcgcctcg
     1081 gagcacgccg cgccttggtt ggggggtatg tcctgggcac actgtcctgc gtgctccttg
     1141 cgatcatctt tgccctcggt tattggaaca ttctaccttt ggcggcagtg ttcatcctct
     1201 cgggaatctg tgccgcagtc caagaaacgg tcgaacccgt tgtcacggct gctctcgtcc
     1261 ccgctgaaat ccgcgcgacg tgctatggag tactgggcgc ggtcaatggc gtgggcgatc
     1321 ttatctcaag ctttttcgtc gggctcattt ggacgctcgt tgccccccaa tgggcttttt
     1381 tggccgcagg cggtaccctt ctgatgggga cggcgcttct cgcggcttta aaaaaccacg
     1441 gagcggattc cgcatgagcc cgcttaaccg agtttaactg cccagcggat cacggggctg
     1501 ggatcgtgta actaacgtcc agtccctcgc caggagcagc gaggaaattg gtgatctcgt
     1561 attccagcgg gatgggttgg gaggcgtcag ggacattgat cctaaagatc gccgttgtgt
     1621 tggccttcaa cgtaacttct gccgggccaa attgacctgt cctttttaga acgaggtcca
     1681 tggcgcactt gttgcgaacc tttacccaca cgcttgtttt gcttcgcata tgcgggggat
     1741 caatttccag agaggcctcg gccaaggcgg tgagccattc tggtttcccg atcatcagag
     1801 gtccacacca aacggctgtt cgcccggcac gaagggcctc caacaccccc tgttcgctct
     1861 tctcggccac aaacaccagt gtcatcgtcc gatgctccgc cgaggtgttt ttctggagta
     1921 ggtccggacc gtgaatgtcg gaattgccca ccatcgtcaa ccctttttcc aggcaccaac
     1981 ggtgcgcagt tggatagtag tcgtctccgt tggccacctc catcccgtga aggagcttct
     2041 tctcgtatag tgtggagtgg atatccagcc aacgtccgag ttcttccccc ttccacgcat
     2101 ggtgattcca aaagacgaat cccccttgct cacatccggc ggcaatggcc tccaccaagt
     2161 cgtccttcac aagcgggttg atatccttga ggaaaatcac gttgaagtgt cccggcggag
     2221 tgtcccgagt gatttcggca ccgcgaataa gaatgatccc cagttcccgg gcacggccca
     2281 aggcgacctc gaatggccga ttgtgattgg tcggcacatc ggccttgtga ggttggtact
     2341 cgatgtggtc actcagagcc aagacatcca gcccgacacg gtacgcttcg tccacgcgga
     2401 cggttggcca aacattgccg
//
LOCUS       DAOPTR010000628         2420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_34647_length_2420_cov_2.760677, whole genome shotgun sequence.
ACCESSION   DAOPTR010000628 DAOPTR010000000
VERSION     DAOPTR010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34647_length_2420_cov_2.760677"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PKI05_11460"
     CDS             complement(<1..669)
                     /locus_tag="PKI05_11460"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOA52863.1"
                     /translation="MSTDNTHTMNLGVFLKVFASRAWPDILDAVRAHGLRWVHFTLQA
                     LVGESLPRTISSTIVSEARNDLENRGLRIASFSGTFNMAHPDADYRQEYLKRFATACQ
                     ACRDLGGSTVALCTGTRDPYDMWKGHPENNSPQAWIIMRDTVAAALEIADRYDLTLAF
                     EPEINNVVDSAEKARRLLDELQHPRLKVILDPVNLWDEPSFDRRNVILETAVQLLGRD
                     IVVAH"
     gene            877..>2420
                     /locus_tag="PKI05_11465"
     CDS             877..>2420
                     /locus_tag="PKI05_11465"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOA52864.1"
                     /translation="MPQTGVIHHDAAERLAALANNLYTLPAVALQILELVDRADTDTT
                     VLRQCVEKDPALAAKILRVVNSSLYGPSQPIGDLGQALAFLGVKPLKLLVLGFSLPPR
                     MFQGLEAKVLAYYWRHTLTKAVAARELSEELVRTSSEECFLAGLLQDIGLLVFIQCLG
                     APFQRFVEKVIEKQEDLYAREQEVFGFDHRAISARLLRHWGMPPLIVDIIDPSELPGI
                     SLGASLVQKRKILLAAEFAAKILGDERPEEWPKWEHVTKQLRGTYGGPHPEELLKQIY
                     HKVGQLAEVLSLRLPPGQSYEKLLAAAHDQLAKVSEEVAGLMLRREKPQGSSLAESTD
                     HRSGEAEDLHRALISCLESPSPMLSASSSENPREPIMENHFRQGQSSVVRSNTDKARD
                     GQDAFRTMQAATDDIAKLADPGLLGHLRATVAFCRAKRCGLSLLLADYHNTSDLLMML
                     GEEAFSKLQQFLLTACARLEHPDSLCWPYGDTGCAWILPDCERETAVLYGHQLIRTVG
                     GWRISGR"
BASE COUNT          531 a          674 c          662 g          553 t
ORIGIN      
        1 atgcgccacg acgatatccc gaccgagtaa ctgcaccgcg gtttccagaa taacattgcg
       61 gcgatcgaaa gacggctcat cccaaagatt cacgggatcg agaatgacct tgagcctggg
      121 atgttgaagc tcatcgagca gccgccgcgc cttttctgct gaatcaacga cattattgat
      181 ctccggttca aaagccaaag tgaggtcgta tcgatcggca atttccaggg ccgcagccac
      241 tgtatcgcgc atgataatcc aggcctgtgg cgagttgttt tcagggtggc ccttccacat
      301 gtcgtatgga tctcgcgtcc ccgtacagag ggccacggtc gatcccccaa gatcacgaca
      361 tgcctggcag gccgtggcaa agcgtttgag gtactcttgt cggtagtctg catccgggtg
      421 ggccatattg aatgtgccgg agaatgacgc aatccgcaat ccccggttct cgaggtcgtt
      481 ccttgcttcg gaaacgatcg tggagcttat ggtccgagga agcgactcgc ccaccagcgc
      541 ttgaagcgtg aaatgaaccc aacgaaggcc gtgggcacga acggcgtcca gaatatctgg
      601 ccacgctcgg gacgcaaaga ctttcagaaa aacccccagg ttcatggtat gtgtgttatc
      661 tgtgctcatt ttcgctgctt ttccaaaaat cgccatcttt ttctttcggc acgagctaga
      721 gttgagatga cagctctcat ttggattacc catgatccaa agcaattccc tggacgatcg
      781 aaaataccgt ttcccttagt aaaaccaata tcaccgtcat aatcgatccg gcaaaccaac
      841 cgtgtggaat tgagcacatc gtttggcgta tcaggaatgc cccaaactgg agtaatacat
      901 cacgatgcgg cggagcgatt agcagcgctg gcgaataacc tttacacctt gccggcggtg
      961 gccttgcaaa tccttgagct ggttgaccgc gcggacacgg acaccaccgt ccttcggcaa
     1021 tgtgtggaaa aagacccggc cttggccgct aagattctgc gtgttgttaa tagttccctt
     1081 tatggtccga gtcagccgat cggcgaccta ggccaagcac tggcgttctt gggcgtcaag
     1141 cccctcaagc tgttggtttt gggcttcagc ctgcctccgc ggatgttcca gggcctcgag
     1201 gccaaggtcc tcgcctacta ctggcgacat accctcacaa aagccgttgc ggcccgcgaa
     1261 ttgagcgagg agctcgtacg gacatctagt gaagagtgct ttctagcagg tctcctccaa
     1321 gacattgggc tactggtttt tatccaatgt cttggggccc cttttcaaag attcgtggag
     1381 aaagtgatcg agaaacagga agacttgtat gcccgtgagc aagaggtgtt cggcttcgat
     1441 catcgggcga tctcagcaag acttctgcgt cactggggaa tgccaccgct cattgtggac
     1501 ataatcgacc cgtctgaact accagggatc tcgcttggtg ctagtcttgt ccagaagaga
     1561 aagattctcc tggctgccga atttgccgcc aagattttgg gcgacgaacg gcctgaggag
     1621 tggcccaagt gggaacacgt gaccaagcag ttgcgaggga catacggcgg gccacatcct
     1681 gaggagttac tgaagcagat ttatcacaaa gttggtcaac tcgccgaggt tctttctctc
     1741 cgacttccac cgggacaaag ctatgaaaag cttttggccg ccgcacacga tcagttggcc
     1801 aaggtgtccg aggaggtggc cggattgatg ctgaggcgtg aaaaacctca aggttcgtcc
     1861 ctggcggaat cgaccgacca ccgttccggc gaagccgagg acctgcatcg cgccctgatc
     1921 tcttgtctgg aatccccctc gccaatgctt tctgcttctt cgtcggagaa cccacgcgaa
     1981 cccattatgg agaaccattt ccggcagggt cagtcgtcgg tcgtccggag taacactgac
     2041 aaagcccgcg acggccaaga cgcctttcgt acaatgcaag cagccacaga cgatattgcc
     2101 aaactcgcag atccaggcct cttgggtcac ctgcgtgcca cagtggcctt ctgtcgcgcg
     2161 aaacgttgtg gtctcagcct ccttcttgcc gattaccaca acacgtccga cctccttatg
     2221 atgttgggag aggaggcctt ttccaagttg cagcagtttt tacttacggc atgcgcccgc
     2281 ctggagcacc ctgactctct ctgctggcct tacggtgata cggggtgtgc gtggattctg
     2341 cctgactgtg agcgcgagac ggcagtcctt tatggccatc agttgatccg gacggtcggc
     2401 ggttggcgaa tttcgggccg
//
LOCUS       DAOPTR010000629         2418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_34672_length_2418_cov_4.022006, whole genome shotgun sequence.
ACCESSION   DAOPTR010000629 DAOPTR010000000
VERSION     DAOPTR010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34672_length_2418_cov_4.022006"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PKI05_11470"
     CDS             <1..408
                     /locus_tag="PKI05_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52865.1"
                     /translation="QHPLPGLQFLSAEVLPSGTGKARVERVVYEISVPEDRLPMVQAR
                     IDELRRRSELLLERPGQRSPRNYRSQLEDLSLHNNRLRMTIRVDPQGSVHPREVLGLV
                     GLDDLEADGYVLVRTKVELASEGRRHRGDGQSS"
     gene            448..2058
                     /locus_tag="PKI05_11475"
     CDS             448..2058
                     /locus_tag="PKI05_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOA52866.1"
                     /translation="MKQEMLINAAQPEECRIAIVEDGVLEELYIERANQETYVGNIYK
                     GIVVNLEPSIQAAFVDFGVGRNGFLHVSDVEPQYFRQAGYDPEELGDLPPPASGVLNG
                     VEGGPARASRSRSSRNNIRPHVRPPIQNIFRRGDEVIVQVIKEGIGSKGPTLSTYISI
                     PGRYLVLMPALGRVGVSRKIEDEAVRRRLREIMHQLDLPKGLGFIVRTAGADRTRKEL
                     SRDLAYLLRLWKVIVRRIKKSEAPCDIYQESDMIIRTIRDIFSAEVGTIIIDEPSTYE
                     RAREFLEMVMPRYVNRVQYYDGKEPLFHKYGLEKEINIIHQRQVPLPGGGSIVIDQTE
                     ALVAIDVNSGNFRVDDSAEETAYQINLIAAKEIARQIRLRDLGGVIVNDFIDMRKEKH
                     RRAVERALREAVRRDRARTKILRTSPFGLIEMTRQRIRPSLKRTLYRDCPLCGGAGLV
                     KTVETMAIETMRQLLLAVLADRVAEITVHVHEEVAEFLNNDKRRFITDLETKHHVTIR
                     LVGEKHVSPEHLVIECRSSDGRIIPMPT"
BASE COUNT          506 a          684 c          722 g          506 t
ORIGIN      
        1 cagcatcccc ttccgggctt gcagtttttg tctgccgaag tcctaccgtc gggcacgggc
       61 aaagcgcgag tcgaacgcgt ggtatatgaa atctccgtac ccgaagatcg tctcccgatg
      121 gttcaggctc gcatcgatga acttcgtcgt cgcagcgagt tgctgctgga acgaccgggt
      181 cagcgttcgc cccgtaatta ccgttcgcaa ctcgaggatt tgtctctgca caataaccgg
      241 cttcgtatga ccatccgtgt ggacccgcaa ggttcggttc atccgcgtga ggtcctcgga
      301 ttggtggggc tggatgattt ggaagcagac ggttatgtcc tcgtgcggac aaaggtcgag
      361 ttagcctccg aagggcgtcg tcatcggggg gacggccaat caagctgatg cgcactcgcc
      421 tccagtgccg caaaggatgg cgagtttatg aagcaggaaa tgttaatcaa tgctgcgcaa
      481 ccggaagaat gccggattgc cattgtggaa gatggagttc tcgaagagct ctatatcgag
      541 cgggcaaatc aggagactta cgttggcaac atctataagg ggattgtcgt caacctggag
      601 ccgagcattc aggcggcctt tgttgacttt ggggtaggcc ggaacggttt tctccatgtg
      661 agcgacgtcg agccgcaata tttccgccag gcgggctatg atccggaaga gctaggcgat
      721 ttgccaccgc ccgcttcggg ggttctcaac ggagtggaag gcgggccggc gcgggcttct
      781 cgcagtcggt cctcgcggaa taatatccgg ccgcacgtgc gtccacccat tcaaaacatc
      841 tttcgccgcg gcgacgaggt cattgtccaa gtcattaagg aaggcattgg cagcaaaggg
      901 ccaacccttt ccacctacat tagtatccct ggtcggtatc tcgttctcat gccggcttta
      961 ggaagagtcg gggtctcgcg caagatcgaa gacgaggccg ttcgtcgccg actccgcgag
     1021 attatgcatc agcttgacct gcccaagggg ctggggttca ttgtgcggac ggccggggcc
     1081 gatcgcaccc gcaaggagtt gtcccgcgac ctggcctatc tcctgcgtct gtggaaggtc
     1141 atcgttcgtc gcatcaagaa gtcggaagcc ccctgtgata tctaccaaga aagcgacatg
     1201 atcatccgca cgatccgcga tatcttcagt gcggaggtcg gaacgatcat cattgatgag
     1261 ccttctacgt acgagcgggc cagagagttc ttggaaatgg tcatgccccg ctatgtcaac
     1321 cgggtgcagt attacgacgg gaaggaaccc ctctttcaca agtacggcct agaaaaggag
     1381 atcaacatca ttcatcaacg tcaggtgcca ctgcccggcg gtgggtccat tgtgatcgac
     1441 cagacggagg ccctcgtggc catcgacgtg aacagcggaa acttccgggt ggatgattcg
     1501 gcagaggaga cggcctacca gatcaacctc attgcggcca aggaaatcgc tcggcagatt
     1561 cgccttcgcg acctcggggg cgtgattgtc aacgacttta tcgatatgcg aaaagaaaaa
     1621 caccggcggg ccgtggagcg agccctccgc gaggccgtgc ggcgagatcg ggcacggact
     1681 aagattttgc ggaccagccc cttcggccta attgagatga cgcggcagcg catccgcccc
     1741 agccttaaac gcacgcttta ccgagactgc cccttgtgcg gcggtgcagg cctggtcaag
     1801 accgtggaaa cgatggctat cgagaccatg cgtcaactgc ttttggctgt cctggccgat
     1861 cgtgtggccg agattaccgt tcacgtccat gaggaagtgg ccgagtttct caacaacgac
     1921 aaacgtcgct tcatcaccga tttggagacg aagcatcacg tcaccattag gctggtcggg
     1981 gaaaaacacg tatcgccgga acacctcgtg atcgagtgcc gtagcagtga tggacgtatt
     2041 atccctatgc ccacgtgaat cggccccgcc gtccaacgat ctggtcgccg aggacggcga
     2101 ccctcccggt aatttgggtc atcggggacg atgaccctcg ctaggcgatt gggagggacg
     2161 gcgtcctcgc cgtcccgtgt gcaccggcgc tacggccaaa cagggcaacc acggggggtt
     2221 gcccctacga ttcctcgtgc aaacagaacc ccattgaaag cgcgtggcac gggcatcttg
     2281 cccgtgccgt tgtccgtctc ggttttgacc gcttgtctac aacgggcgtg catgttggca
     2341 ggtaatttgg tcgccgggga cggcgaccct cccaaagcca tcgtggtatt ttagaaccta
     2401 tcctacaacc tcccctgt
//
LOCUS       DAOPTR010000630         2410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_34835_length_2410_cov_2.864544, whole genome shotgun sequence.
ACCESSION   DAOPTR010000630 DAOPTR010000000
VERSION     DAOPTR010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34835_length_2410_cov_2.864544"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1612)
                     /locus_tag="PKI05_11480"
     CDS             complement(101..1612)
                     /locus_tag="PKI05_11480"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA52867.1"
                     /translation="MKGVRVSRRDFVDAAVTATVATIVPRSVLGGPKFVPPSEKIHIG
                     YVGCGTQGLRQLMSALKHPGVRIVAVCDPNRKSDDYPEWGRGELVGKIRAFLDDPDWG
                     AGARGGLCGREVGLEVVSRYYAKEANRASDSNPACRAYSDFRQMLADEKDLDAVYIMT
                     PDHLHGVVAARALKAGKHAITHKPISNVWAEVRFLRDLAKETGLATQLFCAAGQADSS
                     TIAEWIAAGAIGTVREVHNWSTRPFWPQGMTEYPSEVVPVPDGLEWDLWLGPAAERPY
                     HPAYTHAVFRGWYDFGTGALGDMGHYSFYQIFKILKLGCPVSVEATREQFWKIENFTW
                     KKQINRVSYPRASIIHWEFPAEGNRPAITLHWYDGGLRPPLLEELEVDGREMPAEGML
                     FVGDEGKILAGFTGTQPRLIPEKKMKEFQPPPQTLPRPKEEFEQFVLACRGEAKPDAN
                     FESVYPMAETILLGTIAVRVEKKLKWDSTKGEFVNSPEANALRTRENRTGWEL"
     gene            1851..>2410
                     /locus_tag="PKI05_11485"
     CDS             1851..>2410
                     /locus_tag="PKI05_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent oxidoreductase"
                     /protein_id="HOA52868.1"
                     /translation="MRALPCLAVALNCGLVWANDVVWLETEKFADRGGWVNDAQFVDQ
                     MGSPFLLAIGLEGPVADATTTVVIPATATYRVWVRTRDWLPEYSPGRFQVILGEQTVE
                     RVFGANKQERWVWEDGGTVRLQAGPLTVRLHDLTGHYSRCDAIVLAVDPNFRPASDLG
                     QLARQREMFGGVSREIKTLEPYDTVVV"
BASE COUNT          494 a          677 c          702 g          537 t
ORIGIN      
        1 gcgacgaatt caccgccacc gcggaggcca gcagccggtc gatcagcttg ctaagaccga
       61 gattcatgct gacgtaccgt agtagaacac tacgcgtgcc tcaaagttcc catcctgtgc
      121 gattttctcg tgttcggaga gcgttggcct ctggggaatt gacgaactcg cctttggttg
      181 agtcccattt caactttttc tcaacacgca ccgcaatggt ccccaacagg atggtctccg
      241 ccatgggata aacgctttcg aaattagcgt ctggtttggc ctcaccacgg caggccagga
      301 cgaactgctc gaattcctct ttaggccggg gcaaagtttg agggggcggt tggaactcct
      361 tcatcttctt ttccggtatc agccggggct gggtaccggt aaaacctgcg aggatcttcc
      421 cctcgtctcc gacaaacaac atcccttccg cgggcatctc acgaccatca acttccagtt
      481 cttccagtaa gggcgggcgc agaccaccgt cgtaccagtg gagcgttatg gccggtcggt
      541 taccttcggc cgggaattcc cagtggatga tggatgccct gggatacgac acgcggttga
      601 tctgtttttt ccaggtgaag ttttcgatct tccagaactg ctcccgtgtg gcctccacgg
      661 acaccggaca gccgagtttg agtattttga aaatctgata aaagctgtaa tgacccatat
      721 cgccgagtgc cccagtgcca aagtcgtacc atccacgaaa aacggcatgg gtgtaagccg
      781 gatggtacgg tcgctctgcg gcggggccca gccacaagtc ccattccaga ccgtcgggaa
      841 cgggaacgac ctcggatgga tattcggtca ttccctgtgg ccagaacgga cgggtggacc
      901 agttgtgaac ttcacgcaca gtgccgatgg cccctgcggc aatccattcg gcgatcgtcg
      961 aggaatcggc ctgacctgct gcgcagaaaa gttgggtggc caaccccgtt tctttggcca
     1021 ggtcccgcag gaaacgaacc tcagcccaaa cgttggagat cggcttgtgc gtgatggcat
     1081 gcttgccggc cttgagcgcc ctggccgcca caacgccatg gagatggtcc ggcgtcatga
     1141 tataaacggc atcgaggtct ttctcatcgg ccagcatctg ccggaagtcg gagtacgccc
     1201 ggcaggctgg attactgtcg gaggctcggt tggcctcttt ggcgtaatat cgcgagacga
     1261 cttccaggcc aacctcccgg ccacacaacc cgccgcgcgc accggcgccc cagtctggat
     1321 cgtctaaaaa tgctcgaatc ttccccacca gttccccccg gccccactct gggtagtcgt
     1381 cactctttcg attgggatca cacacggcca caatccgaac gccggggtgt ttgagggcgc
     1441 tcatcaattg cctcaaccct tgggttccac accccacata gccgatatgg attttttcac
     1501 tcggcgggac aaacttgggg ccacccagca cggaacgggg cacgatggtc gccacagttg
     1561 ccgtgaccgc ggcatcgaca aaatcacgtc gggagactcg cacgcctttc atggttctca
     1621 tccctttcct gcacgtttgt tcgtagtgga ccgtttaatg gcgaaaaacc tcgaccgtgc
     1681 cctatgataa ccggaagcct aaggcgagaa aattggtcgg cctcttgact atggctttac
     1741 cgtcaagtaa cgtgcttatg cacgtatgat gcgtaagcac gtggcgattg acattgaaac
     1801 ctttttccgt ttgagatcct gcccaaagga agaacaccat gagatgcctg atgagagctc
     1861 tgccttgctt ggcggtagca ttaaattgtg gtttggtttg ggctaacgac gtggtctggt
     1921 tggagaccga gaagttcgcg gaccgcggcg gttgggtcaa cgatgcgcag tttgttgatc
     1981 agatgggttc gcccttccta ttggcgattg gccttgaagg accggtggcg gacgccacga
     2041 cgacggtcgt gattcccgcg acggcgactt atcgtgtgtg ggtccggacc cgcgattggt
     2101 tgccggagta cagccctgga cggtttcaag tgattctggg cgagcagacc gtcgagcggg
     2161 ttttcggtgc caacaagcaa gagagatggg tgtgggaaga tgggggaacc gtccgactgc
     2221 aggccggacc gcttacggtg cgactccatg acctgacggg gcattattct cggtgcgacg
     2281 cgattgtcct ggcggttgat cctaacttcc gtccagcgag tgatttagga caattggcac
     2341 gacagagaga gatgtttggg ggtgtgagcc gcgaaatcaa gacgctggaa ccatatgaca
     2401 cggtggtcgt
//
LOCUS       DAOPTR010000631         2410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_34838_length_2410_cov_2.803397, whole genome shotgun sequence.
ACCESSION   DAOPTR010000631 DAOPTR010000000
VERSION     DAOPTR010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34838_length_2410_cov_2.803397"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(261..1259)
                     /locus_tag="PKI05_11490"
     CDS             complement(261..1259)
                     /locus_tag="PKI05_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HOA52869.1"
                     /translation="MQQADFFVCDIFDAAPKSDMASMEHPVFSISTKPDVTERRYENG
                     RAFLKITPSIKGLATVHDRDILIFCISQIVGALNEGHAVSQTLRFKAFDLLTATNRGT
                     DGRGYEQLRAAFERLAGTRIETNVTTGSVEVLDGFGLIDRYRIVRETRDGRMQEVEVK
                     LSDWVFNAVKSHEVLTLHRNYFRLRKPLERRLYEIARKHCGAQSEWGIGLEKLKYKCG
                     SHSTDKEFKRLLTRIVEEDQAHQHIPDYGLRIDGEKVVFTNRRTMPALLLEDGISQLD
                     AEALHEARVCAPGWDVHYLEREWRQWCAKQEIMPKHPESHFLAFCRSWAEKRGWPS"
     gene            1960..>2410
                     /locus_tag="PKI05_11495"
     CDS             1960..>2410
                     /locus_tag="PKI05_11495"
                     /inference="COORDINATES: protein motif:HMM:NF018691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOA52870.1"
                     /translation="MPTVVIASPKGGAGKSTTAILLGTELAHAGANVIMLDCDPNRSL
                     TLWADRGPLPERMRVLSNVAESDIVKTIKQHDMDGQIVIVDLEGVASRLVSRAISQAD
                     LVVTPMRATTLDATIGIRALQLIAEEEEALDRTIPHAVVFTMTRAIRS"
BASE COUNT          556 a          685 c          590 g          579 t
ORIGIN      
        1 aactcgctct ttttgcgcat acaagcatgc cctgccgggc aatggatcag cagagggcca
       61 gcagaaaaac gcgaggcaac cgacaaatga caaaggcgcg acggggcgac cctcgtcatt
      121 gatctcgata aacaccaaat atgcgccaat tcgtcgtgca atgggtcccg ggttgaggcc
      181 ccgcgtctat cgcttttggt gccttcaacg gccttagtgc ggtttaccaa atcttttcaa
      241 ttcgtttact tgcgtcgggc ctaacttggc catccacgct tctccgccca agagcgacag
      301 aaggccaaaa aatgcgactc cgggtgttta ggcatgattt cttgctttgc gcaccattgc
      361 cgccattccc gctccagata gtgaacgtcc cagcccggag cgcagacgcg tgcctcgtgc
      421 aatgcttcgg cgtcgagttg gcttattccg tcttccagca gcagggcggg catcgttcgg
      481 cggttcgtga agacgacttt ttccccatca atacgcaggc cgtaatcagg gatgtgctgg
      541 tgtgcctggt cctcctccac gatccttgtc aggagccgct tgaactcttt atctgttgag
      601 tgtgaaccac atttgtactt cagtttctcg aggccgatgc cccattcgga ctgcgctcca
      661 caatgcttgc gcgcaatttc gtagagccgc cgctcaagcg gctttcgtag gcgaaaatag
      721 ttccggtgca gggtgagaac ttcatgcgac ttgaccgcat tgaacaccca atccgagagt
      781 ttgacctcga cttcttgcat acgcccatcg cgcgtctcgc gcacgatccg atatcgatct
      841 atcagaccaa aaccatctag cacttcaacg cttcctgttg tcacgtttgt ctcgatgcgc
      901 gttcccgcca gtcgctcgaa tgcagcccgg agctgctcat acccgcggcc atctgtgcca
      961 cggttcgtcg cggtaaggag atcgaaagcc ttaaatcgaa gcgtctggct caccgcatgc
     1021 ccttcgttca aggccccaac gatctgtgaa atacaaaaga tcagaatgtc gcgatcatga
     1081 acagttgcaa gacctttgat tgacggcgtg atcttgagaa aggccctccc gttctcatat
     1141 cggcgttcag tgacgtcagg cttggtggag atcgaaaaca ccggatgctc catagaggcc
     1201 atgtcactct tcggcgcagc gtcgaaaata tcgcaaacga aaaaatcggc ctgctgcatc
     1261 ctgtcgggaa ggagcggcga ccgaacatcc tttgcagtct cgccaaccat ggccctcttt
     1321 cgtgttatca cccaccactg agacagctat cgtgctttca cacaccgccg tcaagatttt
     1381 cgtgttatca cacaccgaat atcgtgttat cacacaccaa gtatcgtgtt atcacacacc
     1441 ggagggaaaa aattagacat atatatcaaa atcttacgcc atgttttcgc taccgtaaca
     1501 catatttaac acatatttaa caccctagcc tgtggataac tttccaggaa agccgtcgcg
     1561 agcttcagtc gctgtagaaa agcaggccaa cggacacccg cgtttgtcgg cagatatgcg
     1621 ccattgcagg ttcatgcgaa tgaagtattg ccctgcccta tcctgcatcc tctctaggcc
     1681 gttgcttggc aacgctcgta aggcgccaag cgatggcgca gcattgagga gcgacaactg
     1741 ttttgaagac tgattcttgg accgttgaaa cgcccttacg aactctccgt tcgcaatccc
     1801 ttttttgttt tcttgtctcg atagctcagg atcatttgat catgaagctg cagacgcatg
     1861 acgccaatcg tcagttcgta cgccgctcaa tgcaaaatag ttattggata attttgtaaa
     1921 tccggttagt ttatttatca gataataaat ggagccttca tgccaactgt tgtcatagcc
     1981 tcacccaagg ggggggcagg aaagtccaca acggcgatcc ttctggggac cgagctggct
     2041 catgccggag caaatgtaat catgctggac tgcgacccta accgttcact caccctctgg
     2101 gccgatcgag ggcccctgcc cgaacggatg cgcgttctgt ctaatgtcgc cgagtccgat
     2161 attgtgaaga cgatcaaaca gcatgacatg gacggccaga tcgtaattgt ggaccttgag
     2221 ggcgttgcat cccgtctggt atcccgagct atctctcagg ccgacctcgt ggttaccccg
     2281 atgagagcaa ccacgcttga cgcaaccatc ggcattcgcg cgcttcaact catcgcggag
     2341 gaagaagagg cattggatcg aacgattcca catgccgttg tttttacaat gaccagggcc
     2401 attcgatcca
//
LOCUS       DAOPTR010000632         2406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_34896_length_2406_cov_3.599745, whole genome shotgun sequence.
ACCESSION   DAOPTR010000632 DAOPTR010000000
VERSION     DAOPTR010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34896_length_2406_cov_3.599745"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PKI05_11500"
     CDS             complement(<1..509)
                     /locus_tag="PKI05_11500"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOA52871.1"
                     /translation="MGNYFLDNDDIQFLFRHIDMKELARLQEAYTDNGQADYAPLDEE
                     DAVDNYWRVLEIVGQIAADIIAPHAEQIDREGNTLNEDGTVTLHPLVRENLRRMAQAD
                     LMGFTLPRKYGGLNCPNLVYTMATEIVSRADASFMNLFGLQGIAETINAFASNDIKDE
                     VLPRFARGEV"
     gene            complement(619..1650)
                     /locus_tag="PKI05_11505"
     CDS             complement(619..1650)
                     /locus_tag="PKI05_11505"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOA52872.1"
                     /translation="MIAPNRQGEVWVFAEQEDGSLHDVVLELCGKARQLADELGVAVG
                     AVLPGHQVRGLASKLIAHGADRVYLVEDSRLAHFQTIPYARVLCTLIEKYRPQIVLYG
                     ATPLGRDLAPRVASAMQAGMTADCTDLQISDVVDPKTKELHPKLLLQIRPAFGGNIIA
                     TIVNYDRWPQMATVREGVMPLLPPNPSRNGEVIEEKVEIDESLLALRLIERHIEPRKV
                     NLKSARVIVAGGGGVGSKEDFRLIYELAGVIGGAVGASRAAVDSGFIGKEHQIGQTGT
                     TVRPALYIAAGISGAVQHRAGMEESAKIIAINTDPNAPIFSIAHYGIVGDLKKVIPMM
                     IKALRERPK"
     gene            complement(1738..>2406)
                     /locus_tag="PKI05_11510"
     CDS             complement(1738..>2406)
                     /locus_tag="PKI05_11510"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52873.1"
                     /translation="DRAILITDRRAAASDTLATSYILAQTIRTIGRYDIVLCGRQAID
                     GDTAQVGPQIAEKLGLPQVTYLEQLVDIVDGQARVRRNVGRGWEIVDVHLPALFTVIS
                     TANQPRPPAARRVMRFKRARIPAEIEAEVRASLGNASDEEVLDAIAKRTEELRRQGLL
                     LEQWNLDDIHADLNRCGLPGSPTKVFRVQAIVLTKKGYTEIPPTEEGIRRLIHELVVD
                     RTLG"
BASE COUNT          511 a          727 c          642 g          526 t
ORIGIN      
        1 acttcaccac gggcaaagcg ggggaggacc tcatccttga tgtcgttgga ggcaaaggca
       61 ttgatcgtct cggcaatgcc ctggaggcca aacaaattca tgaatgaggc gtcggcccgg
      121 ctcacaatct cggtggccat cgtgtaaaca aggttggggc agttgagacc gccgtatttt
      181 cgcggcagcg taaagcccat gagatcggcc tgggccatcc gccgcaggtt ctcgcgcacc
      241 aaaggatgaa gcgtgaccgt cccgtcctca ttcagggtgt tgccttcgcg gtcaatctgt
      301 tcggcgtgcg gggcgatgat atcggccgca atttgaccga caatctccag aacccgccag
      361 taattatcaa cggcatcctc ctcatcgaga ggggcatagt cggcttggcc gttgtccgta
      421 tatgcctcct gcagcctcgc gagttccttc atgtcgatat gacggaacaa aaactgaata
      481 tcgtcgttgt ccaaaaagta gtttcccacg gaaacctctc ccgtcttttc aaacgactgt
      541 caatgtggct gtgtgtcttt cctattagct caacaatcga tccatcatct gcctacgatg
      601 acgtgctcgg aagacaaatc acttaggccg ttcccggagg gccttaatca tcatggggat
      661 gacctttttc aaatcgccca caattccgta atgggcgatc gaaaaaatcg gtgcgttagg
      721 atcggtattg atcgcgatga tcttggccga ctcctccatg cctgcgcggt gttgcacggc
      781 tcctgaaatc ccggctgcaa tatacaaagc gggacgcacc gtcgtccccg tctgcccgat
      841 ctggtgttcc ttgccgataa agccgctatc gaccgcggca cgactggccc ccacggcccc
      901 accgatcacc ccggccaatt cgtaaatcaa acgaaagtct tccttacttc ccacgccacc
      961 accaccggcc acgatgacgc gggcactctt caaattgact ttgcggggtt cgatgtgccg
     1021 ttcgatcagg cggagggcca gaaggctttc gtcaatttcg actttctcct caatgacctc
     1081 gccgttgcgt gagggattcg gaggcaaaag cggcatcacg ccttcacgga cggtggccat
     1141 ttgcggccag cggtcataat tgacgatcgt ggcgatgatg tttcccccaa atgccggtcg
     1201 gatttgcagc aagagttttg gatgcaattc cttcgtcttg ggatcgacga cgtcgctgat
     1261 ctgcaagtcg gtacaatcgg ccgtcattcc cgcctgcatg gcagaggcca cccgcggcgc
     1321 caaatctcgc cccaacggtg tcgccccata gagaacgatc tgaggccgat atttttcgat
     1381 caacgtgcac aaaacccggg cgtaggggat tgtctggaaa tgggcgagtc gggaatcctc
     1441 caccagatag actcggtccg cgccatgggc aatcaattta cttgccaacc cgcggacctg
     1501 atgcccaggc aagaccgcgc cgacggcaac gcccagctcg tccgccaact gccgcgcctt
     1561 gccgcacagt tccaggacca cgtcatgtag ggaaccgtct tcctgttccg caaagaccca
     1621 gacttcaccc tggcgatttg gtgcaatcat cttctttagc ttcttctatc gtcttagaaa
     1681 cctaaccgcg acattttttc aagagaacaa tgacgctgac ttgtccggcc cgaacgatta
     1741 cccaagagtg cgatcgacga ctaactcgtg gataagccgg cggatgccct cctctgtcgg
     1801 tggaatctcg gtatagccct tcttggtcaa gacaatggcc tggacgcgga ataccttggt
     1861 cggagagccg gggaggccac accggttgag gtcggcatgg atgtcatcca ggttccattg
     1921 ttcgagaagt aacccttgcc gccgcaattc ctcagtgcgt ttggcgatgg catcgagcac
     1981 ttcctcgtcg gatgcattgc ccaagctcgc ccgcacttct gcctcaattt cggcgggaat
     2041 acgagcccgt ttgaaacgca tgaccctgcg tgcggcggga ggccgaggct gattcgccgt
     2101 ggagatcacc gtgaagaggg ccggcagatg aacatcgacg atttcccacc cgcgccccac
     2161 attccggcgg acccgcgcct gaccgtcaac gatgtccacc aattgttcaa gataggtaac
     2221 ctggggcaag cctaactttt ccgcaatctg aggtcccacc tgcgccgtgt caccgtcgat
     2281 cgcctgccgg ccgcacaaaa caatgtcata tcggccgatt gtgcggatcg tctgagccaa
     2341 gatgtagctc gtcgccaatg tatcactggc cgccgcccgg cggtctgtaa tcaaaatggc
     2401 acggtc
//
LOCUS       DAOPTR010000633         2406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_34902_length_2406_cov_2.774564, whole genome shotgun sequence.
ACCESSION   DAOPTR010000633 DAOPTR010000000
VERSION     DAOPTR010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34902_length_2406_cov_2.774564"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2171
                     /locus_tag="PKI05_11515"
     CDS             <1..2171
                     /locus_tag="PKI05_11515"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOA52874.1"
                     /translation="GVVNGEQWKPAPAPLMTPWAKDVTPDNVWPEYPRPQMVRSQWQN
                     LNGLWDYAICPKEQEKPSAWDGKILVPFCVESALSGVGKAVQPTERLWYRRTFEKPKV
                     AADQRVLLHFGAVDWETTVWVNGQEVGRHTGGYDPFTFDITSALRDGENEIVLAVWDP
                     TDTGWQPRGKQVLNPKGIWYTAVTGIWQTVWLEVVPSQYIASLKLVPDVDQGLLRVTV
                     NAPTGLGVEVQAFFDRQSVGHAHGTTGSTIELKIPNPKLWWPDEPNLYDLDVKLIQNG
                     QVIDQVKSYFGMRKVSVEKDERGINRLMLNGKFVFQLGPLDQGWWPDGLYTPASDEAM
                     KYDIVMTKKFGMNMARKHVKYECDRWYYWCDKLGLLVWQDMPSGNVNRNEESRANFRR
                     ELKAMIDARFNHPSIIMWVPFNEGWGQHDTCDIVAWIKDYDPSRVVNEASGWHDNGCG
                     DISDMHKYPGPGVRPIEEKRACVLGEFGGLGMPVPGHLWREQGSWGYVAYKDFEALTD
                     AYVRLLTGVRMLIPEGLCAAVYTQTSDVEIEVNGLMTYDRAVTKIDIARAAEAARKLY
                     LPPPVVKTLVPTSQNEPQTWRFTTEQPTGEWFAPDFDDSSWKEGPGGFGTEGTPGTVV
                     RTIWKTPDVWLRRSFELSEVPSEGELWLNIHHDEDAEVYINGVLVASPKGFLTNYTLM
                     PLAAGRIEDILRRGKNVIAVHCHQTQGGQYIDVGLSHVTE"
BASE COUNT          555 a          632 c          704 g          515 t
ORIGIN      
        1 aaggcgtcgt caacggggaa caatggaagc cagcgccggc accactcatg accccatggg
       61 caaaagatgt caccccagat aatgtgtggc cggagtatcc gcgtccgcag atggtccgct
      121 cgcagtggca aaaccttaac ggattgtggg attatgccat ttgtcccaaa gagcaggaaa
      181 agccctcggc ctgggacggt aagattcttg tgccgttttg tgtggaatcg gctttgagcg
      241 gcgtcggcaa ggcggttcag ccgacggaac gtctgtggta tcgccggaca tttgaaaagc
      301 ccaaagtggc ggccgatcaa cgtgtgctcc tccatttcgg ggccgtcgat tgggaaacca
      361 cggtctgggt taacggtcag gaggtaggcc gccacaccgg gggatacgac ccgttcacct
      421 tcgacatcac ttccgccctc cgcgatggcg aaaacgagat cgtcctcgcc gtttgggacc
      481 caaccgatac gggatggcaa ccccgcggaa aacaggtcct caaccccaaa ggtatttggt
      541 acacggctgt aacgggaatc tggcagaccg tctggctcga agtggttccc tctcaataca
      601 tcgcgtcgct gaagctcgtt cccgatgttg accagggact actccgggtg actgtaaatg
      661 ccccgaccgg tcttggtgtg gaagtccaag ccttttttga tcgtcagtcc gtgggccacg
      721 cgcatgggac cacaggaagc acgattgaac tgaagattcc caatcccaag ctgtggtggc
      781 cggatgagcc gaacctctat gacctggatg tcaaactcat tcaaaacggt caggtcatcg
      841 accaggtcaa gagctatttc ggaatgcgta aggtctctgt ggaaaaagac gaacggggta
      901 tcaaccggtt gatgctcaac gggaagtttg tgttccagtt agggccgctc gatcaaggat
      961 ggtggcccga cggcctgtac actccggcca gcgacgaggc catgaagtat gacatcgtca
     1021 tgacgaaaaa gtttgggatg aatatggcta gaaaacacgt caaatacgag tgcgaccggt
     1081 ggtactactg gtgcgacaag ttaggtcttc tcgtttggca ggatatgccg tcgggcaatg
     1141 tcaaccgcaa cgaggaaagc cgtgccaact tccgccgcga actgaaggca atgatcgacg
     1201 cccggtttaa tcatccgtcg atcatcatgt gggtcccctt caacgaaggg tggggacagc
     1261 acgacacctg tgatatcgtc gcctggatca aggattacga tccaagccgg gttgttaacg
     1321 aagccagtgg ttggcatgac aatgggtgcg gggacatttc tgatatgcac aagtatcctg
     1381 gccccggtgt ccgacccatc gaggaaaaac gcgcttgtgt tctcggagaa tttggcggat
     1441 taggcatgcc ggttcccggc cacctatggc gtgaacaagg aagttggggc tacgtggctt
     1501 acaaggactt tgaggccctc acagatgcct acgtgcggct ccttacgggc gtccgaatgc
     1561 tcattcccga aggtttgtgc gcggccgttt atactcagac ttccgatgtt gaaatcgagg
     1621 tgaacggcct aatgacctac gatcgggccg tgacaaagat tgatatcgct cgcgcggccg
     1681 aggcagccag aaaactctat ttgccgcccc cggtggttaa gacgctcgtt ccgacctccc
     1741 aaaacgagcc tcaaacgtgg cgtttcacta cggaacaacc gacgggcgaa tggtttgccc
     1801 ccgacttcga tgattcttca tggaaggaag ggccgggcgg atttggaacc gaaggcacac
     1861 cgggtaccgt ggtccgtacc atttggaaga cccctgacgt ttggctgcga cgatcttttg
     1921 aattgagcga ggttccgtcc gagggcgaac tgtggctcaa tatccatcac gatgaagacg
     1981 ccgaggttta tatcaacggt gttttggtag caagcccaaa gggttttctg acaaactata
     2041 cgctcatgcc gctggccgct ggaaggatcg aggacatctt gcgacgcggc aagaacgtga
     2101 ttgccgttca ctgtcatcaa acccaggggg ggcagtacat cgatgtgggt ttaagccacg
     2161 tcaccgaatg aataccccgg aggcatggct gagccactaa ctggttgttc tcagcagtcc
     2221 gaatgagaag tgaacatcat ttaatcagga tacctgatga catcagtcgg gtatcctgtt
     2281 tttattggtt cgccgccgtc gtgcagtcga gacttcgtgc cgggcaatga gagcaatcac
     2341 cgcggccacc ctcggcaggt tgggcaatgt ggatcttgcg aatctcaacc gtcgtttgaa
     2401 gccccg
//
LOCUS       DAOPTR010000634         2405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_34917_length_2405_cov_3.014043, whole genome shotgun sequence.
ACCESSION   DAOPTR010000634 DAOPTR010000000
VERSION     DAOPTR010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34917_length_2405_cov_3.014043"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..>2405)
                     /locus_tag="PKI05_11520"
     CDS             complement(124..>2405)
                     /locus_tag="PKI05_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52875.1"
                     /translation="APADLQRTPQAQQSERSTPAQGPQTISQVPAAPQRPALAGGQPS
                     DSSLTQMEPLAPGLIGRLAVNGEDVGAILRYIPGESVSQAALDKLIAQYGLTPGEQRL
                     VLMGVIRVQPPQGQPTLAIRMTLTADPNAVADISVQIDGRPISFPATSQEGVSHVGSV
                     LARGDHPIVCQFQGKDLGDTFRLDIAALALPQMPQTGVAASSVKDESLIVGYTAQDLA
                     KVNELPTLGHHALNDELSTLAANELTPGHVAEGTPPPTETPSPPTALAQQGGQPVPPQ
                     LDLLRAREEIRAKYRKDLQEATAPAARALLARTMLKEGNAERDPARQYMLYEEARELA
                     IAAGDPDVAIQAGEAIGLAFDVDPWPMHIAAVNQLSKTARTPQTRENVLGILQPLCDS
                     AIAEDRYDVADSLLDLAINLAGQLRDPVRREQFSRQREEAKRMAAAFEAIQRPLELLK
                     ENPDDPQANEAVGRFYCFVKHAWKKGLPYLAKAETVLLRTVAQSELNRQIGTAARLQL
                     ADDWWALGETLSEAEKAAVRRHAGYYYLQCAGLVSGEAAQRVNTRLAESGRLTDLLGL
                     AAARRATIVGNWQIGPKSLVSPPEPMTQIAFPVIPPAEYDLLVEIQSVPLPPPRGQGG
                     SQRPDPNLDLGRGAFAVGLIQQRRQFLAVTDFQVPNQGLFSFLADYDKKGPDPANPTF
                     RPMSVIRTQRPTLIIYSVRKSDMTVTANGMPIIQYSGDYSQLSMPQGWNSSNQPRIFF
                     ATRLAVYRITRAELRDVTE"
BASE COUNT          485 a          672 c          732 g          516 t
ORIGIN      
        1 acaagccatt caggaccgtg agtcaaagaa gcagcctgat actccaaaac cacatgaacc
       61 agccgaggtt acttcggaat catgagtcgc cagtcgaaag gctcctgtcg gccatagcca
      121 agcctactcg gtaacatcgc gcagttcagc gcgggtgatg cgatagacag caagtcgtgt
      181 ggcaaagaag attcgcggtt gattggatga attccagccc tgaggcatgg aaagctgact
      241 atagtcgccg ctgtattgaa tgataggcat accattggct gtcacggtca tgtccgattt
      301 cctcacactg taaattatca atgtgggacg ttgagtcctg attacggaca tcggccggaa
      361 ggttggatta gcgggatcgg gacctttctt gtcatagtct gcgagaaaag agaaaagacc
      421 ctgattgggg acctgaaaat ccgtcacggc gaggaattgc cgacgctgct ggatcagccc
      481 cacggcaaat gccccgcggc cgagatccaa gttgggatcg ggacgttgcg aacccccttg
      541 acctcgcggc gggggcagcg ggaccgactg gatctctacg aggaggtcat attccgccgg
      601 tgggatgaca ggaaacgcga tttgggtcat gggctctggc ggtgagacca gggattttgg
      661 accaatttgc cagttgccaa caatcgtagc gcgacgcgcc gccgccagac cgagtaagtc
      721 ggtcaagcga ccactctccg ctaggcgagt gttgacacgt tgagcggcct cacctgatac
      781 gagtcctgcg cactgaaggt aatagtaacc cgcatgcctt ctcacggccg ctttttccgc
      841 ctcagatagc gtttccccga gtgcccacca atcgtcggcc aattgtagcc gggcggccgt
      901 tcctatttgt cggttcagtt cggattgcgc caccgttcgc aacagcacgg tttcggcttt
      961 ggccaggtag gggagcccct ttttccaggc gtgtttgaca aagcagtaaa agcggccaac
     1021 tgcctcgttt gcctggggat cgtcgggatt ctctttgagg agttccaagg gacgttggat
     1081 ggcttcaaag gcggcggcca tccttttcgc ctcctcccgt tgccgagaaa actgctcgcg
     1141 acgaacggga tcacgaagtt gaccggcaag gttgatggcc aggtccaaga ggctatcagc
     1201 cacatcatag cgatcttcgg caattgccga atcgcacaat ggctggagga tgcccaagac
     1261 attttcgcgg gtttgaggtg tccgcgccgt cttagaaagt tggttgaccg ccgcgatgtg
     1321 catcggccaa ggatcaacgt caaaggccag gccgatagcc tcaccggcct ggatcgccac
     1381 atcgggatcg cctgccgcaa tggccagttc tcgggcctct tcataaagca tgtactgccg
     1441 cgccggatca cgctctgcgt tcccctcttt gagcatcgtt ctggcaagga gagcgcgcgc
     1501 tgcgggcgcc gtcgcctcct gcaagtcctt tcgatacttc gcccgtatct cttctcttgc
     1561 cctgagaaga tccaactgtg gcggcaccgg ttgcccgcct tgctgggcaa gtgcggtggg
     1621 cggggagggt gtttcggtgg gcggcggtgt accctcggca acgtgccccg gtgtcagctc
     1681 atttgccgcc agcgtgctca gttcatcatt gagggcatgg tggcccagcg ttggcaattc
     1741 gttgactttc gccaagtcct gtgcggtgta tccgacaata agcgactcgt cttttacgga
     1801 cgatgctgcc acaccggttt gaggcatttg tgggagcgcc agcgcggcta tatcgagccg
     1861 aaacgtgtcg cccaagtcct ttccttgaaa ctggcagacg atggggtgat ctccccgtgc
     1921 caaaaccgaa ccaacatggg agactccttc ctgcgaagtt gctgggaagg agatcggacg
     1981 accatcgatt tgcacggaga tatccgcgac ggcattcggg tctgccgtca atgtcatgcg
     2041 aatggctagg gtgggttggc cctgcggagg ctggacacgg atgacaccca ttaggacaag
     2101 ccgctgctcg cctggcgtaa gaccatattg cgcgatcagc ttatccaatg cggcttgcga
     2161 aacgctctcg cctggtatgt aacgaagaat ggcaccaaca tcctcgccgt tgaccgcaag
     2221 tcgcccaatc aggccggggg caagtggctc catttgggtc aaagagctat ctgagggctg
     2281 ccctccagct agcgcgggcc gctgaggtgc ggcaggaact tgcgagatgg tctgtgggcc
     2341 ctgagccggc gttgagcgct ctgactgctg ggcttgtggc gttctctgca aatcggccgg
     2401 ggcgg
//
LOCUS       DAOPTR010000635         2403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_34940_length_2403_cov_3.948041, whole genome shotgun sequence.
ACCESSION   DAOPTR010000635 DAOPTR010000000
VERSION     DAOPTR010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34940_length_2403_cov_3.948041"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PKI05_11525"
     CDS             <1..359
                     /locus_tag="PKI05_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52876.1"
                     /translation="CRQFACTWLKRTDWPTDWRPDRSGLLCLTEHLVSGISGSAVYEM
                     IPGRLSSPLGQAIVAAVARLSHFVTCITATGRRYNHPVEYHEPVPFRRVAEPHFVQIA
                     RKAARKWDGTRGHRTR"
     gene            complement(394..>2403)
                     /gene="acnA"
                     /locus_tag="PKI05_11530"
     CDS             complement(394..>2403)
                     /gene="acnA"
                     /locus_tag="PKI05_11530"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HOA52877.1"
                     /translation="EAEAAMLGQPLSMLAPEVIGLELSGELQPGVTVTDLVLTVTELL
                     RREGVVGKFVEFFGDGVSGMSVADRAVLANMAPEYGATMGYFPIDSQTLEYLRLTGRA
                     PEHVNLVEWYAKEQCLFRSPGDATPAYTKVLRLDLSTVVPSLAGPKRPQDRVPLHELK
                     KSFQSSLRTPVEKRGFGLDESASQRSVKVNLDGQEVSLYHGSVVLAAITSCTNTSNPA
                     LMIAAGLLAKKACERGLRVPAYVKTSLAPGSRVVTEYLQKAGLIPYLEKLGFAVVGYG
                     CTTCIGNSGPLPDSIVEAAQQQIVLAAVLSGNRNFEGRIHSAVKANYLLSPPLVVAFA
                     LAGSVNKDLTSEPLATDTQGKPVFLKDIWPTEDEIRDAVQRSVSPEVFRQKYADLFEG
                     ESLWEDLTIQASELFPWSEESTYIHEPPYFKDFTLREPGVPSIQGARVLLALGDSVTT
                     DHISPAGSIPKTSPAGRYLIERGIPPHEFNSYGARRGNDLVMVRGTFANVRLRNFLAA
                     GTEGGFTRFWPTGEVMPVFDAAMRYKELGTPLIVLAGSEYGTGSSRDWAAKGPALLGI
                     RAVLAASFERIHRSNLVGMGILPLQFPSGTTWQSLGLTGEEVFDIVLPQGGLTPRSSV
                     RVVARKPDGQVLEFACIARIDTPVELDYYRHGGILPFVLRRLLQS"
BASE COUNT          528 a          702 c          654 g          519 t
ORIGIN      
        1 tatgccgaca gtttgcctgc acctggctga agcgaactga ctggcccacg gactggcgtc
       61 ctgaccgttc tggcctgctc tgtttaactg aacacctcgt ttcgggtatt tcaggcagtg
      121 cggtgtacga aatgataccg ggccgacttt ccagcccttt gggacaggcg attgtggcag
      181 ccgttgcccg gctttcccat tttgtgacat gtattacggc aaccggccgc cgttacaatc
      241 atccggtcga gtaccacgag ccggtaccct tccgtcgcgt cgcggaaccg cacttcgtgc
      301 agattgcgag gaaagccgct cggaaatggg acggcacacg cggtcacagg acacgttaat
      361 tgccagcggg caacacgtcc cttggagtgg atgctaactt tgtaagagtc tccgcagaac
      421 gaaaggcagt atgccgccgt gacggtagta atcgagttcc accggtgtat cgatccttgc
      481 aatgcaagcg aactcaagga cctgaccgtc cggttttctt gccacgaccc ggacagaaga
      541 tcgaggagtg agtccacctt gaggcagtac gatgtcaaac acctcttctc ctgttaaccc
      601 aagggactgc caggttgtgc ccgagggaaa ttggaggggt agaatgccca ttcccaccag
      661 gttgctccgg tgaatgcgtt caaagctggc agcaaggacg gcacgaatcc cgaggagggc
      721 cggacctttg gccgcccaat cgcgactgct tcccgtgccg tattcgctac cggcaaggac
      781 gatcaaagga gtgccaagct ctttatatcg catggcggcg tcaaagaccg gcatcacttc
      841 cccggttggc caaaaccgcg taaatcctcc ttctgtcccg gcggccaaaa aatttcgcag
      901 gcgcacgttg gcaaaggttc ctctgaccat gaccaggtcg ttgccgcggc gggccccata
      961 actgttaaac tcgtgaggag gtatccctcg ttcgattaga tagcgtccgg ccgggctcgt
     1021 ctttgggata ctgcctgccg gggaaatatg atcggtagtc accgagtcac ccaaggccaa
     1081 caggactcgt gccccttgaa tggatgggac accgggttcc cgcagagtga aatccttaaa
     1141 atagggtggc tcatggatgt aggtactttc ttcggaccac ggaaacaatt cgctcgcctg
     1201 gatcgtcaaa tcttcccaga ggctttcgcc ttcgaagaga tccgcatatt tttgccgaaa
     1261 cacttccggc gataccgatc gctgaacggc gtcccgtatt tcgtcttctg tgggccaaat
     1321 atcctttaaa aagaccggtt ttccctgcgt gtccgttgct aagggttccg aagtcaagtc
     1381 tttattgacg ctcccggcca aggcaaatgc cacaaccaaa ggcggtgaaa gtaaataatt
     1441 cgctttgacg gcggaatgga tgcgaccttc gaagttccga ttgccactca acaccgctgc
     1501 gaggacgatc tgttgctgag cggcctcgac aatggaatct ggcaagggac cactattacc
     1561 gatacaagtg gtacatccat atccgaccac ggcaaaaccc agtttttcaa gatatgggat
     1621 gagccccgct ttttgcaggt attctgtcac cacgcgagaa cccggcgcca ggctggtctt
     1681 gacataagcc ggtacacgta gaccgcgttc acaagccttt ttggccaata gtcccgcagc
     1741 gatcatcaag gcgggattac tcgtgttggt acaactagtg attgcggcga ggaccacaga
     1801 accgtgataa aggctaacct cttgcccgtc caggttcacc ttgaccgacc gctgcgacgc
     1861 agattcgtcc aggccgaagc cccgtttttc caccggcgtt cgtagcgatg actgaaaact
     1921 cttcttcaac tcgtggaggg gcactcgatc ctgtgggcgt tttggtcctg ccagcgaggg
     1981 aaccaccgtc gataagtcaa gcctcaagac cttggtatag gccggggttg cgtccccggg
     2041 actacggaac agacactgtt ccttcgcgta ccattccacc aggttgacgt gctccggcgc
     2101 gcgccctgtc aggcggaggt attctagggt ttgggaatcg atcggaaagt agcccatcgt
     2161 cgctccatat tcgggagcca tgttggccag cacggcccga tccgcaacac tcatgccgct
     2221 aacgccatcg ccgaaaaatt cgacgaattt tccaaccacg ccctcccggc gaaggagttc
     2281 cgtcacagtc aagaccaagt ctgtgaccgt gacgcccggc tgaagttctc cgctcaactc
     2341 cagcccgatc acttcaggcg cgagcatcga aagaggctga ccgagcatgg ctgcctcggc
     2401 ctc
//
LOCUS       DAOPTR010000636         2403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_34955_length_2403_cov_2.126491, whole genome shotgun sequence.
ACCESSION   DAOPTR010000636 DAOPTR010000000
VERSION     DAOPTR010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34955_length_2403_cov_2.126491"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PKI05_11535"
     CDS             <1..441
                     /locus_tag="PKI05_11535"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF012677.2"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOA52878.1"
                     /translation="FKEAATPQFREYARQVVANARRLAQRLMEKGYQLVSGGTDTHLL
                     VMDFRDRPFTGKDVAQALAKAGIIANFNMVPGDPRKPFVTSGVRLGTPALTTRGMKEP
                     EMDRVAEWIDRVCQNLDRIDEIAPQIRAEIAELCRQFPPPGLLV"
     gene            complement(525..1484)
                     /locus_tag="PKI05_11540"
     CDS             complement(525..1484)
                     /locus_tag="PKI05_11540"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOA52879.1"
                     /translation="MIGWVFRRDFLKLAAIAGAKTLVGPSLFLRASEAASPQPPSEGL
                     TAYQEGPQIWVRWNNEILTSYRAHSTQKYPYFFPLTGPLSGLSLTTESSLPWPHHRSM
                     LFACDRVNRGNYWQGPVSLGQIVSQGPQLGQITPASVEILDECRWAPPNGEVVLSDRR
                     RFVVRLLSPTIRCIDADITWKAETNITIEPTNHSLFAVRAAPDITPLGGGHLVNAEGL
                     SGEKETFGKASSWCCYWGTRARTDIVEGIALFDHPQNPWAPTPWFTRDYGFISPTPFN
                     FIKQPWSMPGGTTLQLRYRVVLFGGNPEQAQLAALYEEWAKTS"
     gene            1803..>2403
                     /locus_tag="PKI05_11545"
     CDS             1803..>2403
                     /locus_tag="PKI05_11545"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOA52880.1"
                     /translation="MERRGFTLVELLVVIAIIGILIALLLPAVQAAREAARRSQCTNN
                     LKQIALAAHNFHDAYKRLPCGTNDPLWVQGFTKYGTTSRIDCVDVYSFRVTLLPYVEQ
                     RALYDVITAYCKQASQTNPYVWNRDNGNCNIPLPWDPFDMYDGRTNPFETYIAAYVCP
                     SDGNANTSQRTNVLGCGNYVGNRGDAPVGDTWRETRGLFG"
BASE COUNT          533 a          635 c          711 g          524 t
ORIGIN      
        1 tttaaggagg ccgccacgcc gcaattccgc gagtacgcac gacaggtggt ggctaatgcc
       61 cgacgcctcg ctcagcgact gatggaaaaa ggttatcaac ttgtcagcgg tggcacggac
      121 acgcacctct tggtgatgga ttttcgggac cggccattca cgggcaagga cgttgcccag
      181 gccctggcca aggcgggaat tattgccaac ttcaacatgg tgccgggcga tccacgcaag
      241 ccatttgtca ccagtggtgt aaggctggga acaccggccc tcacgacccg cggaatgaag
      301 gaaccggaaa tggaccgcgt ggccgaatgg attgaccgcg tgtgtcaaaa cctcgatcgg
      361 atcgacgaaa ttgccccgca gattcgcgcg gaaattgccg agctgtgccg ccagtttccg
      421 cccccggggt tattggtttg atttgcggcg caaccggata aacgcggctt caccccgtgt
      481 cgggaccgtc aggaaattta gctcggctct ctattccgcc tagtttatga tgtcttggcc
      541 cactcttcgt agagggctgc cagttgggcc tgctcgggat tcccgccgaa gaggaccacg
      601 cgatagcgaa gctgaagggt cgtccctccg ggcatggacc acggctgttt gatgaaatta
      661 aatggcgtgg gcgagataaa gccgtaatca cgcgtgaacc atggggtcgg tgcccagggg
      721 ttttggggat gatcgaagag ggcaatcccc tcgacgatgt cggttctcgc ccgcgtcccc
      781 caatagcaac accaactcga cgctttaccg aaggtctcct tttctccgct cagcccttcg
      841 gcattgacga ggtgaccgcc gcccagtggc gtgatgtccg gcgctgcccg cacggcgaac
      901 agcgagtggt tggttggctc aatggtgatg ttggtttctg ccttccaggt aatgtcggca
      961 tcgatgcaac gaatggtcgg ggagaggaga cgaacgacga agcggcggcg atcgctgaga
     1021 acgacctccc cgttgggcgg cgcccagcgg cattcatcga ggatttctac gcttgccggc
     1081 gtgatctggc ccaattgcgg tccttgggaa acgatctgtc ctaagctgac cggtccctgc
     1141 cagtaattgc cgcgattgac gcggtcacag gcaaagagca tggaacgatg atgcggccaa
     1201 gggagtgaac tctccgtagt cagggagagg ccagagagtg ggccagtcag cgggaaaaaa
     1261 taaggatact tctgtgtgga atgggcgcga tagctggtga ggatttcatt gttccagcga
     1321 acccagattt ggggaccttc ctgataggct gtcaagccct cactgggagg ctgaggcgat
     1381 gcggcctcac tggcgcgaag gaacagcgaa ggcccgacca gggttttcgc gccagcaatg
     1441 gcagccagct taagaaaatc acgacgaaaa acccagccga tcatgggagc tgtctcctca
     1501 aagaaggtca ctcacgttcg aaaaccatgt gacatcatat ccagaacgga cacgcgtttc
     1561 caggtatgtc aattaccccg atgtttttct tcagaaatcc gagacatcga taccctgtga
     1621 atgaacgtgg tgcacatttt gctagatatc gcaactcatc ttgtttgtgg gtagcaagtg
     1681 acaaaatgct tgacgtattg ggtccacggc gctatactgg gaatgtaatc gggtagctct
     1741 agtcctgggc tatttctgtt gggaatggtt ggttgatgtc ttgcaatgtg gaggacttag
     1801 ttatggaacg tcgagggttt acactcgtcg aattgctggt ggtcattgcc atcatcggta
     1861 ttctcattgc gttactactg cctgcggtgc aagcggcgcg ggaggccgca cggcggagtc
     1921 agtgcaccaa caaccttaag caaattgctt tggcagcaca taactttcac gacgcgtaca
     1981 agcggttacc ttgcggcaca aacgatcctt tgtgggtcca aggcttcacg aaatacggga
     2041 caacgagtcg gatcgattgc gttgatgtct attccttccg agtcacgttg ttaccgtacg
     2101 tggagcagcg ggccctttac gatgttatca cggcttattg taagcaagca tcccagacga
     2161 atccctatgt ttggaaccga gataacggta actgcaacat cccgttacct tgggatcctt
     2221 tcgatatgta cgacggacgc accaacccgt ttgagaccta catagctgcc tacgtttgcc
     2281 cgtcagacgg caatgccaat acctcccagc gaaccaacgt cctaggctgt ggtaactacg
     2341 tgggaaaccg gggcgacgct ccggtgggcg acacatggcg ggaaacccgg ggattgtttg
     2401 gac
//
LOCUS       DAOPTR010000637         2401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_34977_length_2401_cov_3.682438, whole genome shotgun sequence.
ACCESSION   DAOPTR010000637 DAOPTR010000000
VERSION     DAOPTR010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34977_length_2401_cov_3.682438"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..1050)
                     /locus_tag="PKI05_11550"
     CDS             complement(61..1050)
                     /locus_tag="PKI05_11550"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOA52881.1"
                     /translation="MLPEKKWVLELTDGFRVAAIVGAAAELGLFEVIPEAGITAEGLA
                     SQLSCSLRGVRILCDALTALSILEKQGDLYYLPAKLRPVLRETEPDTAIPMLRHRMAM
                     MRGWAQLAWTVKAGFPAPRTSSIRGPDGDLASFVLAMNVVSRDVADDIVRQLQPLQFR
                     RMLDVGAGPGTWAFAFLRAVPEATAVLFDLPHAIALARRKAEEAGLADRIEFVVGDFY
                     VDELPQDVDFAWVSAITHQNSREQNRQLFQKVFRALRPGGQIAVRDIVLEPDRTAPLE
                     GALFAVNMLVSTPSGNTYTLDEFREDLLAAGFSEPHLRIRAENMNSIILAHRR"
     gene            complement(1116..2132)
                     /locus_tag="PKI05_11555"
     CDS             complement(1116..2132)
                     /locus_tag="PKI05_11555"
                     /inference="COORDINATES: protein motif:HMM:NF024906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52882.1"
                     /translation="MSLSAPRSLIAIRYWVIVICISILATSGCQRETTNGPRSESTPQ
                     ATGSAQGERPTGQPPREGDSALAGKLEALGVRVRLDDDGYIISVDASSSGIDDAQLAI
                     LKDAHRIRSLKLTGCPITDAGLDTIATLINLKSLGLDQTQVTDAGLAKLQSLPHLEEL
                     FLAGTAISDEAVKAISACKKLRRLRLRGTKITDAGLSYLKALPELTEIDLSETAITDQ
                     GLMVLSEMPSLSRLNLYTTQVTDAGMAAFEKMPQLVWLNLDNTKITDAGLPHLKGLTR
                     LEFLHLGRTAISDNGIPRLFDLKALKTLHVTRTNVTEAGGEKLRQALPNCEVITQVQE
                     SPQT"
BASE COUNT          533 a          656 c          666 g          546 t
ORIGIN      
        1 aagcttcagg cgagccacct tcgtcaaagt aactcactga ccagccgcac cagacatcac
       61 ttagcgccga tgcgcgagaa tgattgaatt catgttttcc gctcgaatcc gcagatgcgg
      121 ttccgaaaag ccggcggcca acaagtcttc gcgaaactcg tccaaagtat aggtgtttcc
      181 agatggagtt gacacaagca tattgaccgc gaacagggca ccctctagag gggccgtacg
      241 gtcaggctcg aggacaatgt cacgaacggc aatttgacct ccgggtctga gggcccgaaa
      301 gaccttttgg aagagctgcc ggttctgctc gcgagagttt tggtgggtaa tcgcgctgac
      361 ccaagcgaag tccacatctt gtggcaattc atcgacgtag aaatcgccca cgacaaattc
      421 gatccgatcg gcaagtcctg cttcctcggc tttccgcctt gccagcgcga tggcgtgagg
      481 cagatcaaag aggacagccg tagcctccgg gactgcgcgg agaaacgcga acgcccaggt
      541 tccgggcccg gcgcctacgt ctaacatccg ccgaaattgg agcggctgga gttggcgcac
      601 aatatcatcg gcgacatcgc gagaaacgac attcattgcc aagacgaaag atgccaaatc
      661 cccatccggt ccgcgaatac tcgaagtgcg aggggccgga aaacctgcct tgaccgtcca
      721 ggccagttgt gcccatcccc gcatcatggc catacgatgc cgcagcatcg gaatggctgt
      781 gtctggttcg gtttcacgca acacagggcg gagctttgcc ggtaggtaat atagatcgcc
      841 ttgcttttcc aaaatggaaa gcgcagttag ggcgtcgcac aagatcctga caccgcggag
      901 cgaacacgag agctggctgg ccaagccttc agctgtgatt cccgcttccg ggatgacttc
      961 gaataggcca agctcagcgg cagcaccgac aatcgctgcc actcgaaaac catccgtcaa
     1021 ttccagaacc catttctttt ccggtagcat gtttactcct tgataccctt atgtgccgga
     1081 ctgttgaaca cgtctcttta ggtgacggcg cttcactagg tttgtggcga ctcttgcacc
     1141 tgggttatga cttcacaatt cggaagggcc tggcgaagtt tctcgccgcc cgcctccgtg
     1201 acgttcgttc ttgtgacatg gagcgtcttg agggctttga gatcgaagag acgggggatt
     1261 ccgttatcac tgatggccgt tcgaccgagg tggagaaact caaggcgggt caaacccttc
     1321 aggtggggaa gccctgcatc ggtgatcttg gtattatcta ggttgagcca cacgagttgc
     1381 ggcattttct cgaaggccgc catcccggca tccgtgacct gcgtggtgta aagattaagc
     1441 cgcgaaagac tgggcatttc actcagcacc atcaggcctt gatctgtgat ggctgtctcg
     1501 ctcaaatcga tttcggttaa ctccgggagt gccttgaggt agctcaagcc tgcgtccgta
     1561 atcttggttc cccgcaaacg gagccgtcgc aacttcttgc aagccgagat cgcttttacg
     1621 gcctcatccg atattgcggt cccggccagg aataactctt ccaaatgagg tagtgactgt
     1681 aacttggcaa ggcccgcgtc ggttacttgg gtttggtcca ggccgagact cttgagattg
     1741 atcaacgtcg caattgtgtc taatcctgcg tcagtgattg ggcaaccggt tagcttcaac
     1801 gatcggatgc gatgagcatc tttgaggatg gcaagttggg cgtcgtcaat cccactggag
     1861 ctcgcatcta ccgaaatgat gtagccatca tcatccaggc ggacacgtac cccgagggct
     1921 tcaagcttgc cggcaagggc tgagtcaccc tcacgcggtg gttgaccggt aggccgttcc
     1981 ccttgagcag agccggtggc ttgcggtgta ctctccgagc gtgggccatt tgtggtttca
     2041 cgctggcatc cgcttgtggc gagaatggaa atgcaaatga cgatgaccca ataccgaatg
     2101 gctatcaaag atcgtggggc agataaactc atgacttttg atttcgttaa cgggcttttg
     2161 aaggttattt ggccagtatt acttcgcgaa ccggaccagc atccgcgaaa tcacataagg
     2221 tatcattaca cagagtttga aatctcgacg caatccgtac ctgaaaaaag gacattaaag
     2281 cctgtgccat aatctctcct tgcgcgttcg gcgggagacg ggtcgggagt gagggtggct
     2341 cggatggctg ccggttttct caattgattc cgacgttcgc ttgccctcac cccaaccctc
     2401 t
//
LOCUS       DAOPTR010000638         2397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_35056_length_2397_cov_4.132365, whole genome shotgun sequence.
ACCESSION   DAOPTR010000638 DAOPTR010000000
VERSION     DAOPTR010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35056_length_2397_cov_4.132365"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PKI05_11560"
     CDS             <1..592
                     /locus_tag="PKI05_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOA52883.1"
                     /translation="ITLGLYGLSRNTTTSAIIAAGLGFYLPDLVVTFLGRRRKEAIFL
                     SLPDALDLLVVCVEAGLGLDQAMRKVTEELASSHPVIAKEFALANLQLQMGRPRAEVL
                     HEMGVRTGVQDVRALAAVIIQAEKFGSSIAQALRAQSHAMRTRRRQLAEERAQKTAVK
                     LIFPLVLFIFPGIFVVLVGPAAITMIRELFPKMAGG"
     gene            768..>2397
                     /locus_tag="PKI05_11565"
     CDS             768..>2397
                     /locus_tag="PKI05_11565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOA52884.1"
                     /translation="MMEKEEEIFEGKWYAHPRMRNALIAGLLTGAAFSLAHLGIIPDF
                     AEIAIYAVAILLGGYHWSREGIEEFVTEGEIGIEVLMIAATVGSAVLGMWDEAAFLVF
                     LYGTAEGLEEYTYARTRFSIRKLLDLAPKEARVLRDGEEITIPAEELKIGDILVVKPG
                     ESIATDGIIIEGRSSINEAPVTGESIPVEKKEGMKVFAATVNQEGALKLKVTATFKDN
                     TLSKMIHLVEESQEQKGKAQLFIEKFGKRYSPLVFLSALLLIVVPSLLGAPVSYWATR
                     AVVFLVAAAPCALVMSTPVAIAAGITRAGRSGVLIKGGICLENLGKIKAVAVDKTGTL
                     TKGEPVVTDVVPLNGEESEVLSLAYNVERYSEHPLAKAIIRKAEESRIRSQEVADFSA
                     IAGYGAKARVGNRVVYVGKEGLFRKLGLESQTIPQIETLRSHGKTVMLVGTETGIDAI
                     VAIRDRIRSEAKEAIDELHRMGIKVIMLTGDNEATARAIAEELGIDEVRADLKPEGKV
                     AAVKMLEEKYGTVAMVGDGINDAPALAQATVGIVM"
BASE COUNT          582 a          554 c          697 g          564 t
ORIGIN      
        1 gattacactt gggctttacg gcctttcgcg caacactaca acatcggcga ttattgcggc
       61 cggtttggga ttctacctgc ccgacctcgt tgtcaccttc ctagggcgac gccgtaagga
      121 ggcaatcttc ttgagcctgc cggacgccct tgacctgctt gtcgtttgcg tggaggccgg
      181 tttggggctt gatcaggcca tgcgaaaagt cacggaggaa ttggccagct cgcatcccgt
      241 gatcgccaag gaatttgccc tggcgaatct ccaactgcaa atgggtcgcc cccgggcaga
      301 ggtcctccac gaaatgggag tccggacggg agttcaggat gtgcgggcat tggcggccgt
      361 catcatccag gccgaaaagt ttggctcaag catcgctcag gcccttcgtg ctcaaagtca
      421 tgcgatgcga acccgacgcc gtcaattggc tgaagaaagg gctcagaaaa cggcggtgaa
      481 gctgattttc cccttggtgc ttttcatctt tccggggatt tttgtggtcc tcgtcggacc
      541 tgctgccatc acaatgattc gtgaattgtt cccgaagatg gccggcgggt agtatcactc
      601 ttatcctgag aacgcaaaac cgtctgctca aaatcggcat tcgtggagcg tgtcgcaggc
      661 gatggcaaat gccgccaagt tgacgactct tccacggatg ccttaaattt gttggttagg
      721 ctgctacttt gccagctgtg gattcaatga gcagcgggaa tgtgcacatg atggagaaag
      781 aagaagaaat ctttgagggc aaatggtatg ctcaccctcg aatgcgaaac gccttaattg
      841 ccggactact taccggtgcc gcatttagtc tggctcattt gggaattatt cccgacttcg
      901 ctgagatcgc catctacgct gtagctattt tattgggcgg ttaccattgg tctcgtgaag
      961 ggattgagga atttgttacg gaaggagaaa tcggcattga agtcctcatg atagccgcca
     1021 ctgttggctc agctgtgctt ggtatgtggg atgaggctgc ctttttggta ttcctctatg
     1081 gtactgcgga aggacttgaa gaatatacct atgcccggac caggttttcc attagaaagc
     1141 ttcttgacct ggcgccgaag gaggcacgtg tcctaagaga tggagaagaa atcaccatcc
     1201 ctgctgagga actcaagata ggggatattc ttgttgtcaa gcctggcgag tccatcgcta
     1261 cggatggcat tattattgaa ggccgttcca gcattaatga ggcacccgta accggagagt
     1321 ctatcccggt ggagaaaaaa gaggggatga aggtctttgc ggctaccgtc aaccaagagg
     1381 gagcattgaa gcttaaggtt actgccacct tcaaggacaa caccctctcg aaaatgattc
     1441 atctggtgga ggaatcacaa gagcagaaag gaaaggcaca actctttatt gagaagtttg
     1501 gaaaaaggta ttctcctctt gtctttctga gtgctttact cctaattgtt gtcccctctt
     1561 tgttgggagc acccgtctcc tattgggcca ctcgggcggt tgttttcctc gttgcggcgg
     1621 ctccctgtgc cctggtgatg tccactccgg tcgccattgc cgctggcatt acaagggcag
     1681 gccgcagtgg agtactcatc aagggtggga tttgtctcga gaacttgggc aaaatcaaag
     1741 ctgtggctgt tgataagacg ggaaccctca ccaaagggga accggtcgtg acagatgttg
     1801 tgccgttaaa tggggaggaa tcagaggttc tcagtttggc ttacaatgtg gaaagatatt
     1861 ctgaacatcc tttggccaaa gcgatcatca gaaaggcaga agagtcgcgg atcagaagtc
     1921 aagaggttgc ggatttcagc gccattgcgg gatatggggc caaagcaaga gttggcaata
     1981 gggtcgtgta tgtgggaaag gaagggttat tcagaaaact cggcctggaa agccaaacca
     2041 tcccacaaat cgaaactctc agaagccacg gcaagacggt catgctcgtc ggaacggaaa
     2101 caggcattga cgctatcgtt gccatcaggg atagaatccg gtccgaagca aaagaggcga
     2161 tagacgaact tcatcgcatg gggattaagg tcatcatgct aacgggggac aatgaagcca
     2221 ctgcgcgggc cattgcagag gaactgggga tagatgaggt cagagctgac ctcaagcccg
     2281 aaggcaaggt ggcagccgtg aaaatgttgg aggaaaagta cggtacggtg gctatggtag
     2341 gagatggcat aaacgatgcc ccagctttgg cacaggcaac ggtaggaata gtgatgg
//
LOCUS       DAOPTR010000639         2390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_35200_length_2390_cov_3.483512, whole genome shotgun sequence.
ACCESSION   DAOPTR010000639 DAOPTR010000000
VERSION     DAOPTR010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35200_length_2390_cov_3.483512"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PKI05_11570"
     CDS             <1..654
                     /locus_tag="PKI05_11570"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOA52885.1"
                     /translation="TLNGWVVKCKPQDNDKRGYWKVVDGAIMADVPPGSPHNYIWLMT
                     EKEFADFELCLKVQTFSTSTGNSGVQVRSRYDDEAYYMDGPQVDINPPGPWRCGFIYD
                     ETRGAQIWLWPDVGRPANAKPEHAPKGWKWFHADHPDTKDAWNDLRIVCHGFRIQTFV
                     NGVPIADYMGQGRLDDDAHQLRRVGLTGHIALQIHPGGTLQIRFKDILLRPIRNAGE"
     gene            complement(712..>2390)
                     /locus_tag="PKI05_11575"
     CDS             complement(712..>2390)
                     /locus_tag="PKI05_11575"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOA52886.1"
                     /translation="LWPVGTLIPLSLAGVCLVLTKSRSAYLALLIGIIVLLGASRALR
                     QRQWRLARFVVISLLVAATGIAVFLRGLDWAVLSEAGKSLSFRWQYWVATVQMIKDHP
                     LFGCGPGNFRYAYLHYKVPEASEEVSDPYNFVLELTATAGIPAGLIFSMGLLATMGQL
                     VRLGWCGHSGREPVSTVAANAVSENAKERQVSPWTAEILALGAGLLLAWPVGWLFGLL
                     SSAPQTATFILVGCPVAILSLITLWPWIENGQWTPLIALVSLVALSVHWLAAGGLTFP
                     GVAYSFWLLWALAQDVIEETSRAVSTSPNAQPPSHKWTFPWGASTKEVPVSKVGAFAA
                     FGAMAGAGLLCYFTAYAPVLDARGLMAEAARIKAVFPPRAEELLRAAAKADPWAYEPW
                     LRRLELWVNTIAPSDNRDNSPRAKTAATPSAIIQPLWADPTAELDECAVNLLRRAKNH
                     HVVRWNLAIAYQKLAQVTGSADPLHRAIKYAADAIRLYPTQAIYYVGYADMLRQAGRL
                     REARQAAQKALWLHEVTPHQDRKLPDETVTSLNAILKLDPNKETDRQPPP"
BASE COUNT          578 a          698 c          653 g          461 t
ORIGIN      
        1 acactaaacg gctgggtcgt caagtgcaag ccgcaggaca acgacaaacg tggctattgg
       61 aaggtcgtcg atggcgccat catggcagac gtccctccag gcagccccca taactatatt
      121 tggctcatga ccgagaaaga gttcgctgat tttgagctgt gcctaaaagt ccaaactttc
      181 tccacttcca ccggtaacag tggggtgcag gtccggagtc gctatgacga cgaggcatat
      241 tacatggatg ggccacaggt ggatatcaat ccgcctggtc catggcgttg cggcttcatc
      301 tatgacgaaa cacggggggc acaaatttgg ctatggcctg acgttgggcg tcccgccaac
      361 gccaaaccag aacacgcgcc gaagggatgg aaatggttcc acgccgatca tccagacaca
      421 aaagatgctt ggaacgatct tcgcatcgtg tgccacgggt tccggattca gacattcgtc
      481 aacggtgtac caatcgcaga ttacatgggc caagggcgac tcgatgatga cgcccaccaa
      541 cttcgccggg taggattgac cggccatatc gccctgcaaa ttcatccggg aggtaccctc
      601 caaattcgct ttaaggatat tctccttcgc ccgatccgca atgccgggga atgagcgcgg
      661 ttccttctct tcaggggttt accgcgaaat cgaaggcaac ctggagacga cttaaggcgg
      721 cggctgacgg tcggtttcct tatttggatc aagcttgagg atcgcattca agctggtaac
      781 agtttcatca ggtaatttgc gatcctggtg gggtgtaacc tcgtgcaacc aaagggcctt
      841 ttgggcggcc tgccgggcct cgcggagacg tcccgcctgc cggagcatgt cggcatatcc
      901 gacgtagtag atggcctgag tgggataaag acggatggcg tccgcggcat atttaattgc
      961 acgatgaagc gggtctgcgc ttcccgtcac ctgcgccagt ttttgatatg caatcgccaa
     1021 attccagcgg acgacgtggt ggtttttcgc ccgcctgagc agattcaccg cgcactcgtc
     1081 caactctgcc gtaggatcag cccaaagagg ctggattatt gcacttggcg tcgccgctgt
     1141 tttcgccctc ggtgaattgt cgcggttatc cgaaggcgca atcgtattca cccaaagttc
     1201 cagtcgccgc agccacggtt cgtaagccca ggggtcagcc ttggcggcgg cacgcaaaag
     1261 ttcttctgcc ctaggaggaa aaacggcctt aatcctggct gcttcggcca tcaaaccacg
     1321 ggcatcaagc acgggagcat acgccgtgaa ataacaaagg agccccgcac ccgccatcgc
     1381 cccaaaggcc gcgaacgccc cgacctttga taccgggacc tccttcgtgg acgctcccca
     1441 cgggaaagtc cacttgtgag acggtggttg agcgtttggc gaggttgaaa ctgcacgaga
     1501 agtctcctcg atcacgtcct gagccaaggc ccaaaggagc caaaacgaat acgctacacc
     1561 gggaaatgtt agccctcctg ctgcgagcca atgaaccgat aaggccacca gactgaccaa
     1621 cgcaatgagg ggagtccact gaccgttctc gatccagggc cacaatgtta tcaggctaag
     1681 gatcgccacg ggacaaccca cgagaataaa tgtcgccgtt tgtggggccg agctgagcag
     1741 gccaaacaac cacccaaccg gccatgccaa caacaaaccg gcccccaggg ctaagatctc
     1801 ggccgtccag ggggacactt gccgctcctt ggcgttttca ctcacagcgt tggcagcaac
     1861 ggtggaaacc ggctcacgcc cactgtgtcc acaccaaccg agtctgacaa gctgccccat
     1921 ggttgccaaa agccccatgg agaagatcaa acccgcaggg atgcccgcag ttgccgttag
     1981 ctccaggaca aaattgtacg gatcggagac ctcttcactc gcctctggca ctttgtagtg
     2041 aagataagcg taacggaagt tgcccggacc acatccgaat aagggatggt ccttaatcat
     2101 ttgcacggtt gccacccagt actgccagcg aaagctcaat gattttccgg cctcggagag
     2161 aacagcccaa tcgagaccgc gtaaaaaaac ggcaatgccc gtcgcggcca ccaaaagact
     2221 gatcacaacg aaacgggcaa gtcgccactg tctttgccgt aaagctcggc ttgccccaag
     2281 caagactatg atcccgataa gtaaggcaag ataggccgag cggcttttgg tcagaaccaa
     2341 acacaccccc gctaggctga gaggtatcag cgtgccaaca ggccagagga
//
LOCUS       DAOPTR010000640         2387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_35259_length_2387_cov_3.498285, whole genome shotgun sequence.
ACCESSION   DAOPTR010000640 DAOPTR010000000
VERSION     DAOPTR010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35259_length_2387_cov_3.498285"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..>2387
                     /gene="mgtA"
                     /locus_tag="PKI05_11580"
     CDS             28..>2387
                     /gene="mgtA"
                     /locus_tag="PKI05_11580"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HOA52887.1"
                     /translation="MLRSLPRNMIEALRTRSKEETRVAQALAPHEKLLIEICTLTPGE
                     ALARLATKWSGLDEADVEARLDEYGHNELPHAQRLSFWADILHRCRNPLNIQLFVIGG
                     VSGLIGEWKSAIVVAAMVLLSIGLSYLLDRRSNRAVEALGKRVQPRSLVLRGGREVEV
                     KISEIVPGDIVLLHAGAMVPADVRLLSAKDFFVSQSVLTGESMPVEKTVFLNGSAATS
                     PWDLPNACFLGSNVISGTARAVVVNTGTRTMLGSISARLIETRPETSFDQGIRGFTWL
                     MIRFMVVMVALVFLIVGFTKGNWVEALLFGLSVAVGLTPEMLPMIVTVNLAKGALSMA
                     KRKVIVKHLPAIQNFGAMDVLCTDKTGTLTQDRVVLERHVDITGRPSDDVLTYAYLNS
                     YFQTGLRSLIDHAVLDRANLDVESSQCRLVDELPFDFQRRRMSVVVDYEGDHVLICKG
                     AVEEIYECCRYYQIGDEIYPLIPVIQNDLFEEVERLNREGFRVLGVAYREFPRDKTTF
                     SVADESDLILLGYIAFYDPPKDSALEAIRLLKEVGVTVKVLTGDNALVTEKVCHDVGL
                     EVRRIVSGNELADLTEEEFDRVVREVDVFVKLSPSQKEQIVRKLREAGQVVGFLGDGI
                     NDAAAMKAADVGISVDSAVDVAKEAADIVLLEKNLLVLEEGVMEGRRIFANIVKYIRM
                     GASSNFGNMFSVLGASYLLPFLPMKPIHILVNNLLYDVSQTGIPSDNVDAEQVSRPLK
                     WDIDNIRRFMVFIGPISSVFDYLTFALMWFIFGCSAYLQGGTDREY"
BASE COUNT          501 a          605 c          684 g          597 t
ORIGIN      
        1 agaaggtgag ggctcgggga gacattcatg ctccggtcgc ttccacgaaa catgattgaa
       61 gcgctgcgga cccggtccaa agaggagacc agggtcgctc aggcattagc tcctcacgag
      121 aagcttttaa ttgagatttg caccctgacc cccggcgaag cgctcgcccg attggccaca
      181 aaatggtccg gcctcgatga ggcagacgtt gaggcgcgcc tcgatgaata cggccacaac
      241 gaacttcccc acgcccaaag gctttcattt tgggcggaca tcctacaccg gtgcagaaat
      301 ccgctaaaca ttcagctttt tgtcattggg ggtgtttcgg ggctcatcgg tgaatggaaa
      361 tcggccattg tcgtcgcggc gatggttctc ctcagcattg ggctttctta tttgctcgac
      421 cgccgatcga atcgcgctgt ggaggcccta ggaaagcgtg tccagccccg aagtcttgtc
      481 ctacgtggcg gacgtgaagt tgaggtcaag atctcagaga tcgttcccgg cgatatcgtt
      541 cttctccacg ccggcgccat ggtcccggcc gacgtccgtt tgctctcggc caaggacttt
      601 tttgtcagcc aatcggtact tacaggagaa tcgatgcccg tcgaaaagac ggtttttctc
      661 aacggtagcg cggccaccag tccctgggat cttccgaatg cttgtttcct cggaagcaat
      721 gtcattagtg gcacagcccg cgccgtcgtg gtaaacaccg ggaccaggac gatgctgggt
      781 tcgatctcgg cacggctcat tgaaacgcga cccgagacca gcttcgacca aggtattcgg
      841 gggtttacct ggctcatgat ccggttcatg gtagtgatgg tggccctggt tttcctgatt
      901 gtgggattca ccaaaggcaa ttgggtagag gctttgctct ttgggctttc ggtggccgtg
      961 gggctcaccc ctgaaatgtt gcccatgatc gtcacggtca acctggccaa aggggccttg
     1021 agcatggcca agcgaaaggt catcgtcaag catctcccgg ccatccaaaa ctttggggcc
     1081 atggatgtcc tttgcaccga caagactgga accctcaccc aggaccgtgt tgtcctggaa
     1141 cgtcatgtgg atatcaccgg gcgccctagt gacgacgtgc tcacctatgc ttatttgaat
     1201 agctattttc aaaccggttt gcgaagtctg atcgatcatg cggtactgga tcgggcaaat
     1261 ctcgacgtgg aaagcagcca gtgtcgcctt gtcgatgagc ttccctttga ttttcagcgg
     1321 cgtcgtatgt ctgtcgtcgt tgattacgaa ggggaccacg tcctcatctg caagggcgcc
     1381 gtggaagaaa tatacgagtg ctgccggtac taccagattg gcgacgaaat atatccgctg
     1441 attcccgtta tccaaaacga tttgtttgag gaggtcgaac gactcaatcg ggaaggcttt
     1501 cgtgtgttgg gagttgccta ccgcgagttc ccgcgggaca agacgacttt ttctgtagcc
     1561 gatgaatccg atttgatctt gctaggctat attgccttct atgatccacc gaaggactcg
     1621 gcgctggagg ccatccgact gctaaaagaa gtgggtgtca cggttaaggt tttaacgggt
     1681 gacaatgccc tcgttaccga aaaagtgtgt cacgacgtgg gcctcgaggt ccggcggatt
     1741 gtttcaggca acgagctcgc cgatttgacc gaggaggaat tcgacagggt tgtccgtgag
     1801 gtcgatgtct tcgtgaaact cagcccaagc cagaaggagc agattgtccg caagttgcgc
     1861 gaagctgggc aagtcgtggg tttcttaggt gatgggatta atgatgccgc cgctatgaag
     1921 gccgccgatg tggggatttc ggtggactcc gcggttgatg tggcgaaaga ggccgccgat
     1981 attgtccttc tcgagaaaaa cctcctggtt ttggaagagg gggtcatgga aggcaggcgg
     2041 atttttgcaa atatcgtcaa gtatattcgc atgggggcaa gctccaattt tggcaacatg
     2101 tttagcgttc tcggggccag ttacttgctg cccttcctcc ctatgaagcc catccacatt
     2161 cttgtgaata accttctgta cgatgtgtcg cagacaggca ttccttcgga caacgttgat
     2221 gcggagcaag tttctcggcc cctaaaatgg gatattgaca acatccgccg tttcatggtg
     2281 ttcattgggc cgatcagttc cgtttttgat tatcttacgt ttgccctaat gtggttcatt
     2341 tttgggtgtt ccgcgtatct acaaggcgga actgatagag aatactg
//
LOCUS       DAOPTR010000641         2385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_35297_length_2385_cov_3.163090, whole genome shotgun sequence.
ACCESSION   DAOPTR010000641 DAOPTR010000000
VERSION     DAOPTR010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35297_length_2385_cov_3.163090"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..599)
                     /locus_tag="PKI05_11585"
     CDS             complement(192..599)
                     /locus_tag="PKI05_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HOA52888.1"
                     /translation="MKKKVALIAALLLALVGGLVVQLYAESQAKFSHAGQVRHVVLFK
                     FKDGTSPEDIAKIEKAFAALPSKIPQIAGFEWGTDCSVENLSQGFTHCFILTFKNVQD
                     RDAYLPHPEHKAFGQLLRPHLDKVLVIDYVVRE"
     gene            complement(655..2076)
                     /locus_tag="PKI05_11590"
     CDS             complement(655..2076)
                     /locus_tag="PKI05_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007331546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOA52889.1"
                     /translation="MFRSRVVPLALVAASCVCHIGQVVHGEESSRGRYNVLFVIADDL
                     NTALSTYGHPMVKSPNVDRVQRWGMRFDRAYCQFPLCNPSRASFLTGLRPDHTRVYDN
                     AVHFRKNVPDCVTMPEFFRKQNYFVARVGKLFHYGVPGQIGTSGLDDPQSWDLVINPR
                     GRDKDEEDKIFSLEPGKFGGTLSWLAADGTDEEQTDGIGATEAIRLLEENKDKRFFLA
                     VGFYRPHTPYVAPKKYFSLYPPELVPLPTIPENYFSTIPALAITLKPEEKAMDEKTKR
                     QAIQAYYAAITFMDAQLGRVLDALERLHLRERTIIVMTSDHGYHMHEHGLWQKRTLFE
                     ESARVPLLIAVPGMKTAGQSTQAIVEMIDIYPTLVDLCGFEVPKNLDGMSLVPILNNP
                     AHPGKSHALTQVSRGGGKTRLVMGYSVRTDRWRYTEWDGGKQGRELYDHQDDPQEMKN
                     LAGDERYENVMKELSALMPKPAE"
     gene            complement(2109..>2385)
                     /locus_tag="PKI05_11595"
     CDS             complement(2109..>2385)
                     /locus_tag="PKI05_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52890.1"
                     /translation="SNGIFMDEGSSDIVVEGQTIFNIGRSPLRFHRALEITLSHNTLV
                     CLPGVPPYRYNRTDPKTLRFENDHIVEAEHWTPPSQILGEVGVREGR"
BASE COUNT          564 a          643 c          615 g          563 t
ORIGIN      
        1 caatgccgtc atgggcgcta tcgccgtaga gcgctggcgt gccaagctct ttgcgccgct
       61 tgacctcgat gtggaaccag gggtggttcg acgcgacacc gcttcgcgca aataaaaacc
      121 ctcccgtgac aggagggtac cgcatcgccc accggttcga gacagatgtc gccaaattac
      181 atcgatttga actactcgcg aacgacgtag tcgatcacga ggactttatc caagtgagga
      241 cggagtaact gcccaaacgc tttgtgctca ggatggggta gataggcatc ccggtcttgg
      301 acattcttga acgtcaagat aaagcaatgc gtaaaacctt gagaaaggtt ctccacgctg
      361 caatcggttc cccattcaaa gccggcgatc tgaggaatct tgcttggcaa ggctgcgaag
      421 gccttttcga tcttggcaat gtcttcagga gaggtgccat ccttgaattt aaaaaggacg
      481 acgtgacgaa cttgcccggc gtggctgaat ttcgcttgag attctgcgta cagttgtacg
      541 acgagaccac caacgagcgc aagtaggaga gccgcaatca aggccacttt cttcttcatg
      601 ttgtcctcct ctccaattga aagtaccgaa acaacctaac agtcatcgcc ctagttattc
      661 cgccggtttt ggcatgaggg cacttaattc cttcatgacg ttttcgtatc gttcatcacc
      721 ggccagattt ttcatctctt gaggatcatc ttgatggtcg tataactctc gcccctgttt
      781 gccaccatcc cactctgtat agcgccagcg atctgttcga accgaatacc ccatgacaag
      841 cctagtcttt ccgccaccgc gcgatacctg ggtgagggca tgtgacttgc caggatgggc
      901 aggattattg agaatcggta ccaaactcat cccgtctagg ttcttgggaa cttcaaatcc
      961 gcaaagatcg accaaggtcg gatagatgtc gatcatttcg acaatcgcct gagtcgattg
     1021 gccagccgtc ttcattcctg gaacggcaat aagcagcggc acgcgagccg actcttcgaa
     1081 aagggtccgt ttctgccaca agccgtgctc atgcatgtga tagccgtgat cgctcgtcat
     1141 gacgatgatc gtacgctctc gcagatgaag tcgttctaag gcgtcgagca cgcggcccaa
     1201 ttgtgcgtcc atgaacgtga tcgcggcgta gtaggcttga atggcttgcc tctttgtctt
     1261 ttcgtccatc gccttttctt caggtttcag cgtgatggcg agggcaggga tcgtactgaa
     1321 atagttctcc gggatcgtag gcaaggggac caattcggga ggataaaggc tgaagtactt
     1381 ctttggggcg acatagggtg tgtgcggacg gtaaaacccg accgcgagga aaaatcgctt
     1441 gtccttgttc tcttcgagca gacgaatggc ctccgtggct ccaatgccgt cggtttgctc
     1501 ttcgtcggtc ccgtctgcgg ccaaccaact gagtgtaccc ccaaacttgc ccggttccag
     1561 gctgaaaatc ttgtcttcct cgtccttgtc gcgtccacga ggattaatga ccaaatccca
     1621 agattgaggg tcatcaaggc cacttgtgcc gatttgcccg ggcaccccgt agtgaaatag
     1681 ctttcccaca cgggcaacga aataattttg ttttcggaaa aactccggca tggttacgca
     1741 gtcgggaacg ttcttccgaa aatgaacggc attgtcatac acacgcgtat gatccgggcg
     1801 aagtcccgtg agaaaagacg ctcggcttgg gttgcaaaga ggaaactgac aataagctcg
     1861 gtcgaaccgc atgccccaac gttgcacacg atccacgttg ggggatttca ccatcggatg
     1921 cccataggtc gatagcgctg tgttcaaatc gtcggcaatc acgaaaagca cattgtagcg
     1981 accacgtgag ctttcttctc cgtgcacgac ttggccgatg tggcaaacac aagaggctgc
     2041 cacgagcgcg agcggaacca cacgacttcg aaacataaag cacctcccca aaaaaccaaa
     2101 ccctagattc atcgaccttc gcgcacgccg acttccccaa gaatttgact cgggggagtc
     2161 cagtgttcgg cctcgacgat gtggtcattc tcaaaacgca gcgttttggg atcagtgcga
     2221 ttatagcgat aaggcggcac tcctggcaaa cacacaaggg tattgtggct caacgtgatt
     2281 tccagtgcgc gatggaagcg taaaggactt cgcccaatat tgaaaattgt ttgaccttcc
     2341 acaacgatgt cactgctgcc ttcgtccata aagatgccgt tgctt
//
LOCUS       DAOPTR010000642         2383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_35333_length_2383_cov_3.087629, whole genome shotgun sequence.
ACCESSION   DAOPTR010000642 DAOPTR010000000
VERSION     DAOPTR010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35333_length_2383_cov_3.087629"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="PKI05_11600"
     CDS             <1..523
                     /locus_tag="PKI05_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52891.1"
                     /translation="GVPDVIVKVYAEAKEAVGQVTTDSEGRFSFSAPYRCDYLLRAVM
                     EDGHQAEALIHADELPNTLPALPDQQTQAAVARPTESQTAGQTPGSGVLPLNHTAEAS
                     QRPSAAQSTGTNDESVRQEVASLRSQVIQLREDLKQLQTTTGLRDIFGGVGYILGLTG
                     ITFYFLAKRGRNK"
     gene            576..1346
                     /gene="cbiQ"
                     /locus_tag="PKI05_11605"
     CDS             576..1346
                     /gene="cbiQ"
                     /locus_tag="PKI05_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HOA52892.1"
                     /translation="MNRQWDEGPMLRHSSAAPIDRFDPRLRTLAVGLTALGTVAAGRL
                     LTLLGLLALGLVFLALARMPLSVTVKRCLPLLGFGVLLAMILPWTVPGTPLGTGFWAH
                     ASSEGLTQAGLITLRALIIILATIGLVGTLEIPVLGHAFVHLRIPPKLVFVFLYAVRY
                     LQVLQDEYHRLRTAMVTRGFRPAMNRHTYRAYGYLVGMLLVRSLERSERILTAMRCRG
                     FRGKFYVLDHFHFTSWDVLFGLICVIATGLYFGWEVVW"
     gene            1376..2146
                     /locus_tag="PKI05_11610"
     CDS             1376..2146
                     /locus_tag="PKI05_11610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA52893.1"
                     /translation="MAFTRSVDPQQIGLTAAAAGSLSTQEYSSPSEQEANGLPPVINV
                     RDLEFSFDGQVRLFSHLDFTLSPGEKVGLLGPNGSGKTTFLHILMGLIPACAGTIEIL
                     GRVRRHPSDFRDLPGQVGLLFQDSDDQLFCPTVAEDVAFGPFNQGKSPREVREIVRQT
                     LDKLGLAGWESRITYRLSGGEKRLVALATVLAMEPQLLLLDEPTAGLDESACARVEKI
                     LSALPQSMVIVSHDRGFLQRICHRVLVLKDGRLSLCHA"
BASE COUNT          482 a          660 c          684 g          557 t
ORIGIN      
        1 gggagtccca gacgtgattg tgaaagtcta tgccgaggcc aaggaagctg tcggccaagt
       61 cacgaccgat agcgaaggac gattctcctt ttcggccccc tatcggtgcg attatcttct
      121 ccgagccgtg atggaagacg gccatcaggc cgaggccttg atccatgctg acgaactccc
      181 caacacgctg cctgctcttc cggaccagca aacacaggcg gcagtcgcga ggccgactga
      241 atcccaaacc gcaggccaga cgccggggtc cggggtttta ccgctcaacc acacggctga
      301 ggcaagccaa cgtccaagtg ccgctcaatc caccggcact aatgatgagt ccgttcgcca
      361 ggaggtggcc agcctgcgta gccaggtcat ccaactgcgt gaggatttga agcagttgca
      421 gactaccacc ggccttcgcg atatttttgg gggcgtgggt tacattcttg ggctgacggg
      481 tattactttc tatttcctgg caaaaagagg gcgaaacaag tgacaaatca ttaactcgtg
      541 agatcgtttg atgcaatggg agaaaaatcc tcgatatgaa tcgccaatgg gatgaaggcc
      601 ctatgctgcg acacagcagc gcggccccca tagatcgctt cgatccccgc ctgcggacgt
      661 tggccgtggg cctaaccgcc ttggggacgg tggcggcagg acgcctacta actctccttg
      721 ggctgcttgc gctgggtttg gtctttctgg cccttgcgcg aatgccgctt tccgtgactg
      781 tcaagcgatg tttgccgcta ctgggattcg gcgtccttct ggcgatgatc cttccctgga
      841 ccgttcccgg cacaccgctg ggcacaggtt tttgggccca cgcaagttcg gagggactaa
      901 cgcaggcagg gctgatcacg cttcgggcat taattatcat tcttgccacg atcggtcttg
      961 ttgggacgct ggaaatccct gttctgggcc atgccttcgt tcatctgcgc atcccgccaa
     1021 aactggtgtt cgtgtttctg tatgctgtcc gttatctcca agtccttcag gatgagtatc
     1081 atcggttacg aacggccatg gtaacgcggg gctttcgccc ggccatgaac cgacacacct
     1141 atcgggccta cggttatctg gtggggatgc tcttggtaag aagcctcgaa cgctcggaac
     1201 ggattttgac cgccatgcgg tgtcgtggct ttcgagggaa gttctacgtc ctagaccact
     1261 ttcattttac ctcctgggat gttttgttcg gtttgatctg cgtgatcgct accggccttt
     1321 attttggatg ggaagtcgtg tggtaacaga acaaccccaa cccaaagggg acaagatggc
     1381 gttcacccgc tccgtcgatc cgcaacagat aggtctgacg gctgccgcgg ctggttcatt
     1441 gtccacgcag gaatattctt ctccttcgga gcaagaagcc aacggcctgc cgcccgtcat
     1501 taacgtccgt gatttggagt ttagcttcga cggccaagtc cggctgttct ctcatctcga
     1561 tttcacatta tctcctggcg aaaaagtggg gctactcggg cccaatggat cgggtaagac
     1621 gacgttcttg cacatcctca tggggcttat cccggcttgt gccggcacaa tcgagattct
     1681 ggggcgggtg cgtcgtcacc ctagcgattt tcgtgatctg ccgggacagg tgggactgct
     1741 tttccaggat agtgatgacc aactgttttg ccccacggtg gcagaggatg tcgcctttgg
     1801 ccccttcaat caggggaaaa gcccacgcga ggttcgggag attgtgcgtc aaacgttaga
     1861 caaacttggg ctggccggat gggagtcgcg gattacctac cgattatccg gcggcgaaaa
     1921 gcgattggtt gccctggcaa cggttctcgc catggagccc caactgctgc tcctggatga
     1981 accaacggcc ggtctagacg aatcggcttg cgcgcgggtg gaaaaaatcc tttccgcgtt
     2041 gccacaatcc atggtgattg tgtctcatga ccgcgggttc ttgcagagaa tctgccaccg
     2101 ggtccttgtg ttgaaggatg gtcgcctcag cctgtgccac gcataatggg cataagtgga
     2161 caacgcacag ctaagatatt cgaagtggga ctggcacaag ccagcatttg aaccgttggg
     2221 ggcatcttaa ttgcgcatct aatgcagact tcatggcgag gcaagtattt cgccaccggc
     2281 ccgggtcgct aaagcctgcc acatcgcttt ttcgatggtt tgggaataga accgggcaga
     2341 gccgtcaaca tgaaccgcat tcaccccacc aggatggtaa ctg
//
LOCUS       DAOPTR010000643         2381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_35364_length_2381_cov_3.368874, whole genome shotgun sequence.
ACCESSION   DAOPTR010000643 DAOPTR010000000
VERSION     DAOPTR010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35364_length_2381_cov_3.368874"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..>2381
                     /locus_tag="PKI05_11615"
     CDS             175..>2381
                     /locus_tag="PKI05_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOA52894.1"
                     /translation="MPDEPTIPQGGAERRLSQAMSLQGRRPPADVPGYDPERLLGAGA
                     YGEVWVALERNTGRRVAIKFYSHRGGLDWSLLSREVEKLAFLCADRYVVQLIAVGWDS
                     EPPYYVMEFMEQGSLAERLAKGPLPATEAVRLFREVAIGLLHAHNKGVLHCDLKPGNV
                     LLDQDGKPRLADFGQSRLSHEQLPALGTLFYMAPEQADLNAVPDARWDVYALGALFYC
                     MLTGHPPHRDQETAKKIEEITDLEERLAAYRRLIEESGIPTDHRSIPGVDRGLAAILE
                     RCLAPDPNQRFPNIQSVLVALETWAAERARRPLVVLGFVGPALLLLVFALFAWRGFAT
                     TVNRSRDMLIHRALENNRLMAQYVADLAGKELRRRFEAVEQLGGNARFQDLIRQVIRD
                     PEYEELSRELSRADLPEEEAEVLRRRFRSHPLRAALQREFSALIPPWMRPEESGESSV
                     DDVASWFFCDPNGISTARVPESVTIGRNYAWRSFFTGLDRDMPREWRPAQGEHLRSPK
                     LSAVFRSEATGLWIVAVAAPVIDLRTNEFLGVVALTVRVTRFVELEGTPAQFPVLVEL
                     RPGDHTGMILQHPLFDQVLLREQRLPERFQNYRLSKDALPPDLDKGAIHDPRAIGYRD
                     PLAADPEGLLFAREYLAQAAPVKVSSGNDSRDTGWMVIVQEDYDVAVRGPLEGFQKAL
                     VRYGLISASLVAAILLGLWTLTVRLLNEIHENHLLSRSEYLLRETSRGSHRVVP"
BASE COUNT          458 a          653 c          730 g          540 t
ORIGIN      
        1 ttggggatag gctctaaacc atgttgtgcg ctgtgtcatt tgcggaatga cgacgcacca
       61 ttcttcacgg cgcttcacta aaagacctca ttttggaggg cctaagcgga tatactgaag
      121 taggttgggc caatcaacaa acagccagca agtcttcttt ccccaatcgc atctatgcca
      181 gacgaaccga cgatcccaca aggtggtgcc gaacgccgac tatcacaggc gatgagcctg
      241 caaggtcgcc gtccgcccgc cgatgtgccg ggctacgatc ccgagcgcct cttaggagca
      301 ggggcgtacg gggaagtttg ggtagccctg gagcgcaaca cgggccgtcg ggttgccatc
      361 aagttttact cccaccgcgg cgggctcgat tggtccctgc tttcccgcga ggtcgaaaag
      421 ctggccttcc tctgcgccga ccggtacgtt gttcagctca tcgccgtggg gtgggactcc
      481 gagcctccct actacgtgat ggagttcatg gaacaggggt cgttggcgga acgtctggca
      541 aaggggccgc ttcccgcgac cgaggccgtt cgcttgttcc gcgaggtcgc tataggattg
      601 cttcatgccc ataacaaagg ggtcttacac tgcgacttga agccgggaaa tgtcctcttg
      661 gatcaggacg ggaagccgcg tctggccgat tttggacaat cgcggctgtc gcatgagcaa
      721 ctcccggccc tcggtacgct tttctatatg gcgcccgagc aggccgactt gaatgcggtg
      781 cctgatgctc gatgggatgt ctatgcccta ggggccttgt tttattgcat gctgacgggc
      841 catccgcccc accgggatca ggagacggcc aaaaagatcg aggagatcac cgatctcgag
      901 gagcgattgg cggcctaccg gcgactgatc gaggaatcgg ggattcccac ggaccaccgt
      961 tcgattccgg gtgttgatcg tggactggcg gcgatcttgg aacgttgcct ggccccagac
     1021 ccaaatcaga gattcccaaa cattcagtcg gtcttggtgg ccctcgaaac ttgggcggcc
     1081 gagcgggcac gccggccgtt ggtggttctt ggtttcgtag gacctgcctt gctcctattg
     1141 gtctttgccc tttttgcctg gcggggattt gccacaaccg tcaatcgatc acgcgatatg
     1201 ctgattcatc gggcattgga gaataatcgc ttgatggcac agtatgtggc cgacctagca
     1261 ggaaaggagt tgcgacgccg gtttgaggcg gtggagcagt tggggggtaa tgctcgattc
     1321 caggatttga tccgccaggt catccgcgat ccggaatatg aggaattgtc gcgagagcta
     1381 tctcgtgccg atttgccgga ggaagaagcc gaggtcctgc gtcggcggtt tcgcagccat
     1441 cccctacggg cagccctgca acgggagttt tcggccctta tcccgccgtg gatgcgaccc
     1501 gaggaatcag gggaatcatc ggttgacgat gtggcaagtt ggttcttctg tgatccgaac
     1561 ggcatctcca cggcgcgtgt tcccgaatct gtgacgattg gccggaatta tgcttggcgg
     1621 agtttcttca cgggcttgga tcgagacatg ccacgtgagt ggcgccccgc acaaggagag
     1681 caccttcgtt cccccaagct ctcggcggta ttccggagcg aagcgacggg attgtggatt
     1741 gtggctgtgg cggccccggt gattgacttg cggacaaacg agtttttggg agtggtggct
     1801 ttgactgtcc gcgtgacccg gttcgtggaa ttggaaggca cacccgccca atttccggtt
     1861 ttggtggaac tgcgaccggg agaccacacc gggatgattc ttcaacatcc gctctttgat
     1921 caggttcttt tacgggaaca acgacttcct gagcgatttc agaattatcg cctttctaaa
     1981 gatgcccttc caccggatct ggacaaaggg gcgattcatg atccgcgagc gatcggctat
     2041 cgggacccgc tggccgccga ccccgaagga ttactttttg cacgcgagta tttggcacag
     2101 gccgcgccag tgaaagtctc ctcagggaac gactcacgag acacaggctg gatggtcatc
     2161 gtacaagagg attacgatgt ggcggtccgc ggtccgctgg aaggctttca gaaggcactg
     2221 gttcgctacg gcctgatttc ggccagtcta gtggctgcaa tcctcctggg gttgtggacg
     2281 ctcacggtcc gccttttgaa cgaaattcac gagaatcacc tcttaagtcg ttctgaatac
     2341 cttttgcgtg aaacctcgcg aggatcgcac cgcgtggttc c
//
LOCUS       DAOPTR010000644         2373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_35499_length_2373_cov_3.332183, whole genome shotgun sequence.
ACCESSION   DAOPTR010000644 DAOPTR010000000
VERSION     DAOPTR010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35499_length_2373_cov_3.332183"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2373
                     /locus_tag="PKI05_11620"
     CDS             <1..>2373
                     /locus_tag="PKI05_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52895.1"
                     /translation="LVTICMKCLEKAPERRYPSAAALADDLQRFLEGHVILARPAGPV
                     ERLAKWVRRHSTAVLMGVMTVFAVLGVIFGLRWHFARLQKIEQDRQTLAQLAEKQAEE
                     LRSQEDLSRQRQYVEALARAQELLGDNTAQALAILEDPEKCPEDLRDFAWHLLHRLCH
                     EGRTYWTLAVNQEKWVRRLTLSPDGQYLFWGTWDGTILVWNTLDQRVSHSFTAHSDWI
                     SAFAMVPQNNWLVTASYDHTIRIWQWQPSDEDGPAQLLREFRLEGNDVAWDLTVHPDG
                     QVLLAATDRGIGVFPLSPQVTVESLLAKVNAEVVTVMPPRNAPKQASAGEGDRFFFLP
                     PLATSTEKKTLIVHGQHRLGQAGMFRSVKFSPQGRVVACGAVNGRIVLFSWPQLEILL
                     SLREFQDPVLSIDISRDGNLIAAAAAGNVWLYNMASKQSRYLRDVHFHAIRTVQFSQD
                     GRRLLTAAEDYTAKVWDVKSLAQSHRLTAHIGYAVYGTFSPDGRGLYTASQGMLNLWD
                     LEKHDVPLRLGTDNRTTRAMAWSPDGKHLATVEGTLWRDSSLSLWDVPTRNRLISAKQ
                     NGLSDDWLFFTRDGKWLISVVRERMVEIRDAQTLSVRRVIATLPAPISAMTLSPDGQS
                     LLAGDRRGRIFRWNTVTWLENEIEQMALRNTYATLLLFTPQGDRLLVGTADGRLILWD
                     VPRLRPLASTNLDGGPAAAAFDPLGRWLAVVGRISPAIQLLELSTLERVAIWDETPGF
                     ISHLAFSPDGQALLSATPAEMAAGRVELRVWNVASGRCHAVFPELAGPIL"
BASE COUNT          467 a          671 c          700 g          535 t
ORIGIN      
        1 cctcgtgacg atctgcatga agtgcttgga aaaggcacca gagcgccggt acccctcggc
       61 tgcggccctg gcggatgatt tgcagcgatt cctcgaaggg cacgtcattt tggcgcgacc
      121 cgcaggaccg gtggaacggc tcgcaaagtg ggttcgacgc cactccacag ccgttctcat
      181 gggagtgatg acggtctttg ccgttttggg tgtgatcttt ggcctgcggt ggcattttgc
      241 acgactgcaa aagattgaac aggatcgtca aaccctggcg caactcgccg aaaaacaggc
      301 ggaagaactc cgctcgcagg aagacctcag tcgtcagcga caatacgttg aggccttggc
      361 acgtgcccag gaattgttag gcgacaatac ggcccaggcg ctggccattc tggaagaccc
      421 ggaaaagtgt cccgaagacc tccgcgactt tgcctggcac ctcctccatc gtctgtgtca
      481 tgaaggcagg acctactgga cacttgcggt caaccaagag aaatgggtcc gccgtctcac
      541 gttatcacca gacggccagt acctgttctg gggcacgtgg gacgggacga ttctggtttg
      601 gaacacgctt gatcagcggg tcagtcacag ctttaccgcg catagtgact ggatttccgc
      661 tttcgccatg gttccccaga acaactggct tgtcacggcg tcctacgatc acaccatccg
      721 gatttggcaa tggcagccct cggatgagga tggtccggca caactactca gggagttccg
      781 gctggaaggc aacgatgtgg cgtgggattt gaccgtccat cccgatggcc aggtccttct
      841 ggcagcgacg gatcggggta ttggcgtgtt ccctcttagt ccccaggtta cggtggaatc
      901 gctcctggcg aaggtgaatg ccgaggtagt cacggtgatg cccccgcgaa atgcgccgaa
      961 acaggcatcc gcaggggagg gtgaccggtt ctttttcctc ccacccctgg cgacttccac
     1021 ggaaaaaaag acgctcattg ttcacggcca gcaccgcctg ggacaggctg gcatgtttcg
     1081 ttctgttaag ttctcacccc agggaagggt cgtggcatgt ggagctgtca acggccggat
     1141 cgttctattt tcctggccac aattggagat cctgctttcc ctcagggagt ttcaggatcc
     1201 tgtcttgagc atcgacattt cgcgagatgg taacctaatt gcggcagcag cggcgggcaa
     1261 cgtgtggctg tacaacatgg cttccaaaca gtcgcggtat ttgcgagatg tccatttcca
     1321 cgcaatccgt acagttcagt tctcccagga tggacgtcgc ttgttgaccg cggccgagga
     1381 ttacacggcc aaagtgtggg atgtgaagag tcttgcccag agccaccgtt taacggccca
     1441 catcggttat gccgtgtacg gaacctttag cccagatgga cgcggccttt acacggcgtc
     1501 tcaagggatg ttgaacctct gggatttgga aaagcatgat gttcctttgc gcttgggcac
     1561 agacaaccgg acaacgcggg ccatggcctg gtcgcccgac ggaaaacatc ttgccacggt
     1621 cgaaggaacg ctttggcgag attcatcgct cagcctttgg gatgttccca cgaggaatcg
     1681 tttgattagt gctaagcaaa acggtttatc cgatgattgg ttattcttta cccgcgacgg
     1741 aaagtggctc atttctgtgg tgcgcgaacg catggttgaa attcgcgacg cccaaacgct
     1801 cagtgttcga cgggtgattg ccacacttcc ggcccctatc tcggccatga ccttaagccc
     1861 cgatgggcaa tccttgctgg cgggcgatcg ccgcggtcgg atcttccgat ggaacacggt
     1921 cacctggctg gaaaacgaaa tcgagcagat ggccctccgt aacacttatg ccacgcttct
     1981 cttgttcacg ccacaggggg atcgacttct agtgggcacg gccgatggcc gattgatcct
     2041 ctgggacgtt ccgcggcttc gaccactggc gagcacaaat ctggatggtg gtcccgccgc
     2101 ggcggccttc gatcctctgg ggcgttggct ggccgtcgtc ggtcgaattt caccggcgat
     2161 tcaactcctt gaactttcta ccttagagcg ggtggctatt tgggatgaga cccccggatt
     2221 catctcgcac cttgcgtttt cgccggatgg gcaggctttg ctttcggcaa ccccggcgga
     2281 aatggcagca gggcgagtcg aactccgtgt ttggaatgtc gccagcgggc gctgtcacgc
     2341 cgtttttccc gaactggccg gccccatcct ttt
//
LOCUS       DAOPTR010000645         2371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_35534_length_2371_cov_3.387306, whole genome shotgun sequence.
ACCESSION   DAOPTR010000645 DAOPTR010000000
VERSION     DAOPTR010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35534_length_2371_cov_3.387306"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1353)
                     /locus_tag="PKI05_11625"
     CDS             complement(<1..1353)
                     /locus_tag="PKI05_11625"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOA52896.1"
                     /translation="MDDIVRAFLENEQKLRLVERLGRGGFGEVWAVESSSGVRSAIKV
                     SLDPIDGENPAVKKELENLKLVKALIGHPHIVSLLDYWVVSDYLVTRWELATDGDLLG
                     LWERYREEGQPGIPVEQLIQFISEAADALDFLHGKGIYHRDIKPRNLLLFHGHVKIAD
                     LGLLKLVGASTASHTGSGTFGYLPPEAWQDHRLTSTADLYSLAATYVKLRTGNEPFGT
                     NPVEILARQQRGEPILDGLEAYEKALLLAALDPDPEKRPSEGARNWVRRLYEARLSSS
                     RQAKGQDAVWASVKAQTVPQSREGDSSDVAAPISPGTYVAIQSPGRCSFSPDGRRIVT
                     DSWDDTTTAIVWDARTGERLRVLQGHNDWVYSAAFSPDGRRIVTASNDKTAIVWDAET
                     GERLLVLQGHNGSVYSAAFSPGGQRIVTASIDNTAMVWDARTGERLLVLQGHNGSVYS
                     A"
     gene            complement(1658..1882)
                     /locus_tag="PKI05_11630"
     CDS             complement(1658..1882)
                     /locus_tag="PKI05_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52897.1"
                     /translation="MSQSHPFQPPQLQTLGDVVRWVINELGAMCPSPERLAAYLADPG
                     APELRDVRYHVEEAGCPICRAERDMGRQRP"
     gene            complement(1891..>2371)
                     /locus_tag="PKI05_11635"
     CDS             complement(1891..>2371)
                     /locus_tag="PKI05_11635"
                     /inference="COORDINATES: protein motif:HMM:NF024776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOA52898.1"
                     /translation="GFRPQTGWESWLRALIRSRAIDRLRALERQVFLSWQARTSDVSA
                     NLPGNEPRSPLPSPSREVVEQERRQRQGLLLSEVLAEFCRWCEAKPAGSGQAGRLALK
                     EAYERSLHGQSPADIAAAMGVPRETVYQWLHQARQWVYRRISDKDIDRSVFLTLYGE"
BASE COUNT          459 a          791 c          671 g          450 t
ORIGIN      
        1 cgcagagtaa accgagccgt tgtgcccctg gagcaccaga agcctttctc ccgtccgggc
       61 atcccacacc atggcggtgt tgtcgatcga tgcggtcacg attcgctgcc cgcccggact
      121 gaaggccgca gagtaaaccg agccgttgtg cccctggagc accagaagcc tttctcccgt
      181 ctcggcgtcc cacacgatgg cggtcttgtc attcgatgcg gtcacgatcc gccgcccgtc
      241 cggactgaag gccgcagagt aaacccagtc gttgtgccct tggagcaccc gaagcctttc
      301 tcccgtccgg gcgtcccaca caatggcggt ggtggtgtca tcccacgagt cggtcacgat
      361 ccgccgaccg tctggactga aactgcaccg accgggagac tgaatggcaa catacgtccc
      421 cggcgaaatt ggagctgcga catccgagct gtcaccctcg cggctttgtg gaacagtttg
      481 ggctttgaca ctagcccaaa cggcatcttg gcccttcgcc tgacggctgc ttgagagtcg
      541 agcctcatac agccgccgca cccagttccg cgccccctcg gagggccgct tctccgggtc
      601 aggatcaagg gccgctagaa gtaaagcctt ctcatacgcc tccagcccat ccaagatcgg
      661 ctccccccgc tgctgccgcg ccagaatctc caccgggttg gtcccaaacg gctcgttgcc
      721 agtgcgaagt ttcacgtacg tcgccgccag cgagtagagg tccgccgtgg aagtcaaacg
      781 gtggtcttgc caggcctctg gcggcaaata gccgaacgtc cccgaaccag tgtgcgaggc
      841 cgtgctggcc cccaccagct tcaataggcc caggtccgca atcttcacat ggccgtgaaa
      901 taacagcaga ttccgcggct tgatgtcccg atgataaatg cccttgccgt gcagaaagtc
      961 caaagcgtcc gccgcctcag agatgaattg gatgagctgc tcgacgggga tgcccggctg
     1021 tccctcctcc cgataacgct cccagagacc aagcaggtcg ccatcggtgg ccagttccca
     1081 ccgggtcacc aggtagtcgc tgaccaccca gtaatcaagg aggctcacaa tatggggatg
     1141 tccaatgagg gccttgacca gtttgaggtt ttccagctct tttttgacgg ccgggttttc
     1201 gccgtcgatg gggtccagcg agaccttgat ggcggagcgg acgccgctgg acgattccac
     1261 cgcccagacc tcgccaaaac caccccgccc cagccgctcg acaagccgga gtttctgctc
     1321 gttctccaga aacgcccgaa cgatgtcgtc catcttgcga tgccctcctc gtctctcggc
     1381 tcgccacccc gcgtgtctcc agtcaggccc tccgccccgc tagtgtcaca gcccaggccg
     1441 attttgtgga ggtgcctttg tgttgggtgt cccgtttgag taacggcaca cgatcctctc
     1501 gtgacatgcg gcagcgattt tcaacgcgca ttataaccta cctgacgtat cacggcatca
     1561 caaatgcccg aaccaccagc gtaagccggc ggtgcaagct ttgcgagaga acttagagtt
     1621 taacaggcgt ctcaccgcga gctcacacca gcggttccta cgggcgttgt cggcccatat
     1681 cgcgttccgc ccggcaaatc gggcaaccgg cttcctccac gtggtagcgg acatcccgca
     1741 attcaggggc gccgggatcg gccaaatagg ccgcgagtcg ctccgggctc gggcacatcg
     1801 cgcccaactc gttgattacc caacggacaa cgtcgccaag agtctgaagt tgcggaggtt
     1861 ggaagggatg tgattgactc attgatcatg tcactcgcca tagagcgtca aaaagaccga
     1921 tcggtcgata tccttgtcgc tgatgcgccg ataaacccat tgccgggcct gatgcaacca
     1981 ttggtaaacc gtctcgcgcg gaacccccat ggcggcggcg atgtctgctg gactttgccc
     2041 atgcagcgaa cgttcgtaag cctctttcaa agcaagtcgc ccggcctggc cggacccggc
     2101 cggcttcgct tcgcaccaac ggcagaactc cgccaagacc tcgctgagca ggagcccctg
     2161 gcgctgccgg cgctcttgtt ccaccacctc ccgggagggg gaaggcagcg gcgaacgagg
     2221 ctcgttgccc ggtaaattgg cggaaacgtc gctcgttcgg gcttgccagg agagaaagac
     2281 ctgcctttcc aaagctcgca gacgatcgat cgcccggctg cgaatcaacg cgcgaagcca
     2341 gctttcccag ccggtctgag gccgaaaccc t
//
LOCUS       DAOPTR010000646         2371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_35538_length_2371_cov_2.955095, whole genome shotgun sequence.
ACCESSION   DAOPTR010000646 DAOPTR010000000
VERSION     DAOPTR010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35538_length_2371_cov_2.955095"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="PKI05_11640"
     CDS             <1..999
                     /locus_tag="PKI05_11640"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOA52899.1"
                     /translation="GNIDGIQPKLAVIMIGTNNAAPNQPEETAEGIKAIVQKLRERLP
                     KTKILLLAIFPRGETPEDPLRQKNEQVNKIIAQFADGEMVKLVDIGPAFLTIDGILPK
                     DIMPDLLHPNTRGYSIWAAAIEPYVAEVLGPLPPPQAEPDAEGFVPLFDGKTIAGWRK
                     AGGNATYWTEDGCIVGKVGPGPNTFLCTEKTFSDFILKVEAKFDVPGNSGIQVRSHQR
                     PDTGRVYGYQCEIDPSDRSWSAGIYDEARRGWLFTLENKEDARKAFKRDDWNEFVIEA
                     KGRHLKTYLNGVPCADYVDESDQADLEGFIALQVHRGKQGQIRWRNIRIKVLDKAE"
     gene            1117..1869
                     /locus_tag="PKI05_11645"
     CDS             1117..1869
                     /locus_tag="PKI05_11645"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOA52900.1"
                     /translation="MDVLVLQSGSRGNSVYVESNGVAVLIDAGISARQVQVRLSEYGK
                     SLDAVRAVVISHRHRDHIGHVGVYQRRLRLPVFMTEDGCDVVPDLDETTCHFFRPDQQ
                     LQFEHLRVEPVLTPHDAPPSLAFVVSDEKHRLAILTDLGHPFSQLPAILASVDAAILE
                     SNYDPVLLSEGDYPRWLQDRIRGPGGHLSNKEAALLVKNHGKHLRWICLAHLSQHNNT
                     PEVAYQTYRRVVGNGEPFIADYFSATPLPSLR"
     gene            complement(1965..>2371)
                     /locus_tag="PKI05_11650"
     CDS             complement(1965..>2371)
                     /locus_tag="PKI05_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOA52901.1"
                     /translation="TIGLLTVKYKSADEVPPGRARTRHFSSKRLLTRHGEPGCEEATF
                     QAIAQLQALAAELGMSVTQLALAWLLHRPGVTCVINGMRHPEQARENAVAGDIDLAPD
                     VISRIESITDPIKERLGPNPDMWEGTDRSRFR"
BASE COUNT          530 a          657 c          662 g          522 t
ORIGIN      
        1 ggcaacattg acggcatcca gccgaaactt gctgtgatca tgatcggaac caataacgcc
       61 gcacccaatc aaccagaaga gactgccgag gggatcaagg ccattgtgca aaagctccgt
      121 gagcgcttgc caaagacaaa gattctcttg ctggcgatct tccctcgcgg cgaaacgcca
      181 gaggacccgc tccgtcagaa gaacgagcag gtgaataaga tcatcgccca gtttgccgac
      241 ggtgagatgg tcaagcttgt cgatattggt ccggcgtttt tgacgattga cggaatcctc
      301 cccaaagaca ttatgcccga cttgctccac cccaataccc gaggctattc catctgggca
      361 gcagccatcg aaccgtacgt ggcggaggtc ttgggtccgc tgccgcctcc tcaggcggaa
      421 ccggacgcgg aaggttttgt gccactcttc gatggcaaga ccatcgccgg ctggcggaaa
      481 gctggtggta atgcgactta ttggacggaa gatggctgca ttgttggcaa ggttggtccg
      541 ggtccgaaca ctttcctctg caccgagaag acgttttctg acttcatttt gaaggtcgaa
      601 gccaagtttg atgtgccggg caactcgggg atccaagtcc gaagtcatca gcggcctgac
      661 accggcaggg tctacggtta ccaatgcgaa attgacccct cggatcggtc ttggtcagcc
      721 ggtatttatg acgaggctcg tcgaggatgg ttgttcacgc ttgagaacaa agaagatgct
      781 cgcaaggcat tcaagcgcga tgactggaac gaattcgtca tcgaggccaa agggcgtcac
      841 ctcaagacgt atctgaatgg cgtgccctgt gccgattacg tcgatgaaag tgatcaggcg
      901 gatttggaag gctttatcgc gctccaggtc caccgcggta agcaaggcca aatccgctgg
      961 cggaatatac gcatcaaggt gctcgacaag gctgaatgat tccggcctcg gcagcagttt
     1021 agaggagcct ttcatcaagt ctcgtctagg tgtgggtgat gagcccacat ctagcgagat
     1081 ggttattttt ttctaccaag gagaaagaca ggcttcatgg acgtccttgt tttacagtcc
     1141 ggcagccgcg gcaattccgt ctatgtggaa tccaacggtg ttgccgtttt aattgacgcg
     1201 ggaatcagcg cccgtcaggt ccaggtccgt ctctccgagt acggcaagag cctcgacgcg
     1261 gtacgggccg ttgtgatctc tcaccgtcat cgagaccaca tcgggcacgt cggtgtttac
     1321 cagagaagac ttcgcctccc ggttttcatg acggaagatg gttgcgacgt ggttcctgac
     1381 ctcgatgaga caacgtgtca cttctttcgt ccggatcagc aattgcaatt tgagcacttg
     1441 cgtgtggaac cggttttgac gccgcatgac gcgccgccat ctctcgcctt cgttgtgtca
     1501 gacgaaaagc atcgcttggc catcctgacc gatttgggac atcccttttc gcaactaccg
     1561 gctatcctgg cgagcgtgga tgcagcgatt ttggaaagca actacgatcc ggttctgctt
     1621 tccgaaggcg actatccccg atggcttcaa gaccgtattc gcgggccggg aggtcacctt
     1681 tccaacaaag aggccgccct gctcgtcaaa aaccatggca aacacctgcg atggatctgc
     1741 ctcgcccatc tttctcaaca caataatacg ccagaggtcg cataccagac gtaccgcagg
     1801 gtggttggga acggggaacc cttcatcgcc gattatttca gcgcaacgcc gttaccttca
     1861 ctccgttgat ccggcgaacc cgagcacaaa gaagacttgc ttacccccga ggtggaccgt
     1921 aaacgagcat gtggcagcgt tggtgctccg cggtagctcc aaaatcaccg aaaacgtgag
     1981 cgatctgtgc cctcccacat atcgggattg ggtcctaacc gctccttaat cggatcagtg
     2041 atggattcaa tgcgactgat cacatccggt gccaggtcaa tgtcaccggc aacggcattt
     2101 tcccgagctt gctcggggtg ccgcattcca ttgataacac acgtgacacc gggccggtgg
     2161 agcagccaag cgagggccag ttgggtcacg ctcattccca attcggccgc aagggcctgg
     2221 agctgggcaa tggcctgaaa ggttgcttct tcacaacccg gctcaccgtg tcgcgtcaag
     2281 agtcttttgg aggaaaaatg gcgggtccgt gcacgacccg gaggcacttc atcggcggac
     2341 ttgtatttaa ccgtgagaag tccaatcgtc a
//
LOCUS       DAOPTR010000647         2371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_35539_length_2371_cov_2.913644, whole genome shotgun sequence.
ACCESSION   DAOPTR010000647 DAOPTR010000000
VERSION     DAOPTR010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35539_length_2371_cov_2.913644"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PKI05_11655"
     CDS             <1..558
                     /locus_tag="PKI05_11655"
                     /inference="COORDINATES: protein motif:HMM:NF024761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HOA52902.1"
                     /translation="SAEWAVRVVPSAGDGHDSKSAERVSLPPDVHPESLDLSTALRYF
                     ELPRKLGEHPETGEPVEVWIGRFGPYVKCGQETRSLPADKSPFEITLEECLALLNAPK
                     ASSRRTITELRQLGVSPETGQPVRLMNGRYGPYVTDGQTNATIPREISPDSVTLEQAL
                     QWLTEKAAQGPRKRWRRTSGKRTKK"
     gene            complement(714..1286)
                     /locus_tag="PKI05_11660"
     CDS             complement(714..1286)
                     /locus_tag="PKI05_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOA52903.1"
                     /translation="MKVFTTGQVAKICKVAPRTVSKWFDSGRLKGYRIPGSQDRRIPR
                     EYLIKFLKEHGMPLGELEDEMTAKVLLVAQDQVLIENLKAELPPERSFKVAVASSGFD
                     AGIQAESFRPDCIVVDFSIGRTEALQICQHVRRNTEFNDVILIALLPDDGNAMSFDRS
                     GINETFKKPFDAHLLAERIRTLVGAKKELV"
     gene            complement(1907..2131)
                     /locus_tag="PKI05_11665"
     CDS             complement(1907..2131)
                     /locus_tag="PKI05_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52904.1"
                     /translation="MGGGSRSVAMWFKKHIRLGRDELISKVFLKEKVARLPRTSRISP
                     HIACKNNTAQGGQVCREKRASRVVWMEYEC"
BASE COUNT          549 a          673 c          631 g          518 t
ORIGIN      
        1 tctgcggagt gggcggtacg ggtggtacct tcagcgggtg atggccacga tagcaaatca
       61 gccgagcgcg tgtcgttacc tcccgatgtc catccggaat cgctcgatct gagcacggcc
      121 cttaggtatt ttgagttgcc gcgcaagctc ggggaacatc ccgaaacggg cgaaccggtg
      181 gaagtctgga tcggccggtt tggcccgtac gtcaagtgtg gccaggaaac gcgttcattg
      241 ccggcagaca aatccccatt tgaaatcacg ctggaggaat gcttggccct cctcaacgcg
      301 ccgaaggcat catcgcgtcg aaccattacg gaacttcggc agttgggtgt gtccccggag
      361 actgggcaac ctgttcggct gatgaatggc cgttacggtc cctacgtcac cgatggtcag
      421 acgaacgcca caatcccacg ggagatctca cccgactcgg tgaccttaga gcaggccttg
      481 caatggttga cggaaaaggc agcccaaggt ccgcgaaagc gatggcgccg aacatcggga
      541 aagcgaacaa agaagtaacc cccggtggtg accgcgtggg cgtgctggct cgacaccagg
      601 taacaagaaa taaaaaaccc gcagcacgcg ggctgcggga aatatccgtc gcgggggtcc
      661 ttccgcggcg ggttgtcggg aaccggcgat cgggataggc cgactccttc ggcttacacc
      721 agttcctttt tcgctcccac gagcgtccga atcctttcgg ctagcagatg ggcatcgaaa
      781 ggcttcttga acgtctcatt gatgcccgag cgatcgaagc tcatggcatt gccatcatcg
      841 ggaagaaggg caatcaggat cacatcgtta aactcggtgt tacgacgaac atgctgacag
      901 atctgcaaag cctccgtgcg accgatagaa aagtccacca cgatgcaatc ggggcggaaa
      961 ctctcggctt gaattccggc gtcaaaacca cttgaagcaa cggccacctt gaaggaacgt
     1021 tcaggcggca actctgcttt caagttctcg atgagcactt ggtcctgagc aacgagcaag
     1081 accttcgcgg tcatctcgtc ctcaagctca ccgagaggca ttccgtgttc tttcaaaaac
     1141 ttgatcaggt attcgcgggg aatacgccga tcctgcgaac ccggaatgcg gtatcccttg
     1201 agacggcccg aatcaaacca cttactaacg gtgcgcgggg caactttaca gatcttagca
     1261 acctgacctg tagtgaagac cttcattgca ggctctccaa actgtcactc gcttgagttg
     1321 actaatccct atcggtttca ccaaccgacc ttccacaccg cgttcccgtg tttgcgagtc
     1381 gcctcaccac acgctccttc gtgtggtcct gactcccact tggctcaatt tcgcaaaccg
     1441 ctgggtctcg gaacgctggg aacaccctgt tccaaaagtt tcggccgtgg ctcagctcca
     1501 tctgacccgc gcctcagttc agtaatgaca tcggcaaacc gggccgccga aaaacagtga
     1561 aaattcgcaa aatggggaag gacagtccga cctcggtgac ctctataacg gtcatctcct
     1621 gctggtctgg tgtccctggg gcaaaagaca gaaaccatct tcgcgcagga tttgacactt
     1681 tccgcgcgga cggtcaaata tggtttcccc agtcaatccc cctgggacga ttctcggatg
     1741 gccctccatc catttcgtct cgtcttggag acaacggccc tccgattgat tgtttcgaat
     1801 cgagaatgag aaactcttta agaagtttga cgggttgtag cgaattcgag ggttcgttcg
     1861 aaaagatgga ttatttggga cagtttgaat gtaataaaaa ctcacctcag cattcgtact
     1921 ccatccagac tacgcggctc gcccgtttct cccgacatac ctgcccaccc tgtgccgtgt
     1981 tgtttttaca agcgatgtgg ggagaaatgc gagaagtccg gggaagtctt gcgactttct
     2041 cctttaggaa gactttgctg atcagctcat cccggcccaa gcggatatgc tttttgaacc
     2101 acatggccac agagcgactt ccgccgccca taactctcct ccgaccgaat ggtcgaagaa
     2161 gaaaggcaaa gaaaaggacc tgtttataag ctcgtcagcg gcgaaaacgc tcactgatcg
     2221 cctttgagct gctgacttct tatctcgaac agtacgacgc aagttattga tatatcgaca
     2281 cttatagcat tcgtgggggt gggactgctg catacattgc tgcacttcct ccacggacgc
     2341 ggatggacgg tccttccctt aggccgacgg a
//
LOCUS       DAOPTR010000648         2370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_35551_length_2370_cov_3.843629, whole genome shotgun sequence.
ACCESSION   DAOPTR010000648 DAOPTR010000000
VERSION     DAOPTR010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35551_length_2370_cov_3.843629"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PKI05_11670"
     CDS             complement(<1..723)
                     /locus_tag="PKI05_11670"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOA52905.1"
                     /translation="MRTSLQLIFVLCVAAKGFAETGNLGRDAAPISPAEHQARFNYTR
                     VQPLFETPMRDCAITRGPDGFFFLTGTTGTPRQDGTINFAVNDGIRLWRSHDLKVWED
                     LGLVAPLSLLKAKVEDLGLLRTGRYEDEWKGFIAPELHFVKGGVYLTYALRPCGTGLL
                     KSKSGKPEGPYEDVGLITRVGTDASLFEDDDGTVYWVFGGGWIARINDALTELAEEPR
                     LIQPSTPNANTPGGKILQVGTAG"
     gene            complement(1570..>2370)
                     /locus_tag="PKI05_11675"
     CDS             complement(1570..>2370)
                     /locus_tag="PKI05_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HOA52906.1"
                     /translation="ITRKESFLREFGSHAKINSPQKRHPKNGKSRAKEVATMIVADDN
                     FASIVAGVEEGRIAYDNVRKVIYLLISTGAAEVMMFLTAILTGLRIPLLPVQLLWLNL
                     VTNGIQDVALAFEGGEPGAMKKKPRRPNESIFDSQMVWQTVVSGLTIGGLAFGLWYWL
                     KAFQGLEETAARNLVLLLMVLLQNVHVFNCRSETTSAFRVPFRRNPLLIAGVLIAQGV
                     HILSMYLPPMQKVLQVAPVTLAQWLSLLVLALVILVVMETFKWLRAKA"
BASE COUNT          559 a          668 c          626 g          517 t
ORIGIN      
        1 ccccgctgtc cccacctgca aaatctttcc cccgggcgta ttagcattgg gcgtgctcgg
       61 ttgaatgagc cggggttcct ctgcaagttc cgtcagggca tcgttaatcc gcgcgatcca
      121 ccccccgcca aagacccagt aaacagttcc atcatcatcc tcaaacagcg aggcgtccgt
      181 accgaccctt gtaatcagcc ccacatcctc gtacggccct tcgggtttgc ctgacttact
      241 tttgagcagc cccgtcccac acggcctcaa cgcgtaggtt agataaacgc cacccttgac
      301 gaagtgcaac tctggtgcaa taaacccttt ccattcatcc tcgtaacgcc cggtccgaag
      361 caaccctaag tcttccactt tcgccttcag cagcgacagc ggcgcaacaa gccccaagtc
      421 ctcccatacc ttcaggtcat gggaacgcca caaccgaatg ccgtcattta ccgcgaaatt
      481 gatcgtaccg tcttgccgag gggttccggt cgtcccggtg aggaaaaaaa acccatcagg
      541 cccgcgagtg atggcacaat cccgcatcgg tgtttcgaac agtggctgga cacgggtgta
      601 gttgaacctc gcctgatgct ccgccgggct gatcggcgca gcgtctcggc ccagattgcc
      661 agtctcagca aatcccttgg ccgcaacgca taggacgaag atgagttgta atgacgtgcg
      721 cacgtcgatt ctcctcgcat ggttgtgtag ggactacttg cggtttggta ctcggtgaaa
      781 ctccacagta ctcaggcccc attttgcacc ctgattcttt ggtaagacaa gctagttccc
      841 cacctttacc gagaggcgct caggaacctt gtcttcgcgg gtatccaatt gatttttctt
      901 gtgcggtcag gatacggggc ttttggggtg aaggtaccag aataggccga gtggatgagg
      961 cacccgtcgt gacaggcata acggacacag aacatccggg caacccggcc aagccatttt
     1021 ccgacagcca ccggaacgtt aagccgccgc cgggacaccg gtcttacgta agttaggcaa
     1081 gccagaagag atgttccctg tgcggacggc ttcaagggga atagccgtgg ctcgctattt
     1141 tggaaactga caagcagaaa tcatggcaat ttacccgact cggtgatttt gccaaacttt
     1201 gacacggatg tgaccttgtg gtttagtact atgggactac agtcatatat tgtgtagatg
     1261 ggtcaagccg cagaacaatc ttttcgcgga ttgttttgaa aagacaacac tcctagctgc
     1321 agggaggagc attaccaaag gaagcattcg agcgcacggg taggggcagg acatctcttg
     1381 cccctacggt tggcctgagc tgttgccgga atattaccaa cgggatcgcc gagaaaaaga
     1441 aacgcattgt tttgcgcgag cgggtatggg ttattgcgcg cgaccaggag ggcggcgatc
     1501 aaccccatca ccaatcacct cacgcttttc cgtaatcgct cgccgcattt gccaaaatgg
     1561 ccgcaaatgc taggctttgg ccctcagcca cttgaacgtc tccattacca ccaggatgac
     1621 cagtgctaga accaacaggc tcaaccattg ggcaagggtc acaggcgcga cctggagaac
     1681 cttttgcatt gggggcagat acatgctcaa gatgtgaacg ccttgagcga tcaagactcc
     1741 ggcaatcagg agaggattac gacgaaacgg aacccggaaa gcggatgtgg tctcggaccg
     1801 gcagttgaac acgtgcacat tttggagcag caccatcagt aacagcacga ggttacgagc
     1861 ggcggtttcc tccagacctt gaaaggcctt cagccagtac cacagtccaa aggccaggcc
     1921 gccgatggtc aatccggaaa caaccgtctg ccagaccatc tgggaatcaa agatggattc
     1981 gttgggtctc ctgggcttct ttttcatggc acccggctca ccgccctcga aggccagagc
     2041 tacgtcttga atgccattgg taaccagatt gagccagaga agttgcacgg gtagcaatgg
     2101 aatacgcaaa ccggtcaaga tcgctgtcag gaacatcatg acctcagcgg cgccggtgga
     2161 tataaggaga taaatcactt tgcggacgtt gtcgtaagca atgcgtccct cttcgacacc
     2221 ggcgacgatc gaggcgaagt tgtcatcagc aacgatcatc gtcgccactt ccttagctcg
     2281 acttttgcca ttttttgggt gccgtttttg gggagaattg atcttggcat gggagccgaa
     2341 ttcgcgcaaa aaagactcct tcctggttat
//
LOCUS       DAOPTR010000649         2370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_35553_length_2370_cov_2.895896, whole genome shotgun sequence.
ACCESSION   DAOPTR010000649 DAOPTR010000000
VERSION     DAOPTR010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35553_length_2370_cov_2.895896"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..1562)
                     /locus_tag="PKI05_11680"
     CDS             complement(195..1562)
                     /locus_tag="PKI05_11680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA52907.1"
                     /translation="MSLQRIGQRSRTSLTGQNRRDFLKTSATATAAALTTTSLARFVH
                     AAENNLIQVALIGCGGRGTGAAANALSTQGPIKLVAMADVFQDRLNASLNNLKNSFGD
                     KVDVPPERQFLGLDAYKKAIEALNPGDLVLLATPPAFRPIHLEFAVEKGRHVFMEKSF
                     AVDGPGIRRVLAAGKVAEQKNLKIAGGLMSRHSLPLEQIIERIHDGALGNVISVWAYR
                     MHGPVGFRRRQPGESELAHQIRNYSCFTWLNGSFIVDWLIHNIDVACWVKGDWPVSCQ
                     GQGGRQVRNEPDQLFDHYAAEYTFADGTRMFAQARHMANCWGFWGVVVHGATGSAVLG
                     EGIADPRMYKGYKRVAEDEIWKYEGPPCNPYQRELDLLVDAIRNDKPYNETERCAKSC
                     LAAILGRMAVESGQEVTWEEALQSELSLAPGIDTWKSLDDPAPVQPDENGRYPIAMPG
                     FTKAF"
     gene            1800..>2370
                     /locus_tag="PKI05_11685"
     CDS             1800..>2370
                     /locus_tag="PKI05_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA52908.1"
                     /translation="MTTVLITLLALTTMADAAPRPNVVYIVTDDQRYDALSCAGHPFL
                     KTPHCDQLAQEGARFINAFVTTSLCSPSRASSISGLYAHSHGVIDNFTEFPDDLPSLP
                     KQLQAAGYETAYIGKWHMGEDNDAPRPGFDYWASHNGQGKYFDNEFNINGQRVMLSGY
                     YTHRITELAVDWLKKPRSKPFLLMIGHKAP"
BASE COUNT          546 a          702 c          602 g          520 t
ORIGIN      
        1 cgcttcccaa aactaaagag aaccccgggc cgacaatcaa ccccggggtt ttccgcttca
       61 agaactgtat tcgtcgcacg ttcggcaaaa acgagctggg ccagcgcaaa tagcgctact
      121 ttcccgcgaa gaaacatccc caaccgtctc ccgggtttcg tgacgcgtgc tactaccaaa
      181 ttaatgtagc gtctttaaaa agcctttgtg aagcccggca tggcgatggg gtatctcccg
      241 ttctcatcgg gttgaacggg cgctggatcg tcaagactct tccaagtgtc aataccagga
      301 gccagcgaaa gctcagattg taaggcttct tcccatgtca cctcttggcc cgactcaacg
      361 gccatccggc ccaagatcgc tgctaagcac gacttggcac agcgttccgt ctcgttgtag
      421 ggcttatcgt tgcggatagc atcgacaagc agatcgagtt cccgttgata gggattacac
      481 ggtggacctt cgtacttcca aatctcatcc tccgcgactc gcttgtatcc tttgtacatc
      541 cgcgggtcgg caatcccttc tccgagcaca gcagagcccg tggccccatg cacaacaact
      601 ccccaaaatc cccagcaatt ggccatgtgt cgtgcttggg caaacatgcg tgttccatcg
      661 gcaaaggtgt actctgcggc atagtggtcg aaaagctggt caggctcatt acggacctgc
      721 cgtcctccct gaccttggca ggagacgggc caatcacctt tgacccaaca agccacgtca
      781 atattgtgga tgagccaatc cacaatgaaa ctaccgttca accaggtgaa acagctatag
      841 ttgcgaatct ggtgcgctag ctcactctct cccggctgac gacggcggaa tccaaccggg
      901 ccatgcatcc gataggccca gaccgaaatc acgtttccaa gagccccgtc atggattcgt
      961 tcaatgatct gttccaacgg cagactgtgg cggctcatca agcccccagc gatcttgaga
     1021 ttcttctgct cggcgacctt gcccgccgcg agtactcggc gaattcccgg cccatccacg
     1081 gcgaatgact tctccatgaa gacatgtcgg cctttttcca cagcgaattc taggtgaatg
     1141 ggtcgaaatg ctggaggagt ggccaaaagg acaaggtcgc ccggattcaa cgcctcgatc
     1201 gccttcttgt aggcgtcaag gcccagaaat tggcgctccg gcggaacgtc aactttgtcc
     1261 ccgaagctat tcttcagatt attgaggctc gcgttgagcc gatcttgaaa cacatcggcc
     1321 atggccacaa gcttaatggg accctgagtg gagagggcgt tcgcggcggc cccggttcct
     1381 cggccaccac aaccaataag ggcaacctga atgaggttat tctccgcagc gtggacaaag
     1441 cgggctaacg aggtcgtcgt gagcgcggcg gccgtagcag tcgccgaggt cttcaggaaa
     1501 tcacggcggt tctgcccagt caatgaggtc cttgaccttt ggccgatcct ctgcaaactc
     1561 atggtccttc tccttctgaa cgtttggata caaccggacg gcaccatcac atggccattg
     1621 agagaactgg caacacattg cgttgcggca acgcattgaa gataatccaa acgatggtgg
     1681 gcgtcaatca aacgacgtaa ccgtcggtgg cgtccttgac ttgcgacgga ttacatcgtt
     1741 gtccaagtca tattgggtaa cttctttccc tgtcacgatt tttgctggag aacgcaacca
     1801 tgacgaccgt gctcataacc ctcctggcgt tgactaccat ggccgatgcg gcacctcggc
     1861 cgaatgtcgt ctatatcgtg accgatgatc aacgatacga cgccctaagc tgtgcggggc
     1921 atcctttttt gaaaacaccc cactgcgatc aactggctca ggaaggagca cgatttatca
     1981 atgcctttgt gaccacctcg ttgtgttcgc ccagccgcgc atcttcaatc tcgggcttgt
     2041 acgcccactc ccacggggtg atcgacaact ttaccgagtt ccccgatgac ctcccaagtt
     2101 taccgaagca gctccaggca gcgggttacg aaaccgccta catcggcaag tggcacatgg
     2161 gagaagacaa tgacgcaccg cgaccgggtt tcgattactg ggctagccat aacggacaag
     2221 ggaaatactt tgacaacgag tttaacatca atggccagcg ggtcatgcta tcagggtatt
     2281 acacccatcg tatcacggag cttgctgtcg attggctgaa aaagccccgt tcaaaaccgt
     2341 tcctgctgat gatcggccac aaggctcccc
//
LOCUS       DAOPTR010000650         2367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_35611_length_2367_cov_2.967993, whole genome shotgun sequence.
ACCESSION   DAOPTR010000650 DAOPTR010000000
VERSION     DAOPTR010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35611_length_2367_cov_2.967993"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..1135)
                     /locus_tag="PKI05_11690"
     CDS             complement(29..1135)
                     /locus_tag="PKI05_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOA52909.1"
                     /translation="MIVRHLKKYFPKRRGLLSRVVANTRAVDDVSFAIRRGETLGLVG
                     ESGCGKTTLGRTVVRLLEPTSGTIVFDGQDVLKATGQELRAMRRRMQIVFQDPFSSLN
                     PRMTIKSIIEEGLIVHQRCRELLKANSSTESNNSSTTQTLTRAQAKQKRHERVMKILE
                     EVGLSPKFADRYPHELSGGQRQRVSIARALILRPDFIVLDEPLSALDVSIQSQIINLL
                     VALREQYRLTYLFISHDLSVVQYLSDRVAVMYLGEIVELADSLRLYAEPLHPYTQALL
                     SAIPTMEPGSGRRRIILEGDPPSPENPPPGCRFHPRCPKRMDICSREVPRILDLDGHY
                     VCCHLVEKEVGNKAKIVSDMIAPDPRLPSPGKDE"
     gene            complement(1172..2209)
                     /locus_tag="PKI05_11695"
     CDS             complement(1172..2209)
                     /locus_tag="PKI05_11695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA52910.1"
                     /translation="MTAQIAPTQQPLSTELQPLLRVENLCTYFFTDDGILRAVDDVSF
                     EIKRGRTLGLVGESGCGKSVTALSLMRLMAPPGKIVRGKITFWDAKNKPTVLTDLSDR
                     EMRAVRGRDIAMIFQEPMTSLNPVFTVGSQIIEAIQLHQKKSFSEAKDLTIKLLQQVG
                     IPNAEVRWRDYPHQFSGGMRQRVMIAMALSCNPALLIADEPTTALDVTIQAQILELLK
                     QIQHERQMSILIITHDLGVVAELADDVAVMYASKIVEQASVQELFEQPYHPYTVGLFR
                     SRPRLGQDKRTRLATIEGNVPNPKDFPSGCKFHPRCPYRQKICEQEEPDLREIAPCHF
                     VRCHFAGELRF"
     gene            complement(2246..>2367)
                     /locus_tag="PKI05_11700"
     CDS             complement(2246..>2367)
                     /locus_tag="PKI05_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA52911.1"
                     /translation="NYHYWWLVVFPSLAMFITVTAFNLVGNGLRDAMDPRLRI"
BASE COUNT          516 a          596 c          725 g          530 t
ORIGIN      
        1 gggctgcggg aagaaaagcg gtttcgttct actcgtcttt tcctgggcta ggcagccgtg
       61 ggtcaggggc aatcatgtcg gagacgatct tggccttgtt gccgacctct ttttccacca
      121 agtgacagca gacataatga ccgtcgaggt cgagaatccg cgggacttcc cggctacaga
      181 tatccattcg cttggggcag cgcgggtgaa agcggcaacc gggcggcggg ttctcggggc
      241 tggggggatc accctccagg atgatccggc ggcgaccgct gccaggctcc atcgtgggaa
      301 ttgccgagag aagggcttgc gtgtaaggat ggagcggctc ggcatagagt cggaggctgt
      361 ccgctagttc gacgatctcg ccaagataca tgacggccac gcgatcggac agatactgca
      421 ccacggacaa atcatgagaa atgaaaagat aagtcaggcg atattgctcg cggagcgcga
      481 ccaaaaggtt gatgatctgc gattggatgg acacatccag ggccgaaagc ggttcatcca
      541 gaacaatgaa atcaggtcgg aggatcaggg cgcgagcaat actcacgcgt tgtcgctgtc
      601 ccccggaaag ctcgtgggga tagcggtcgg caaacttggg gctgaggccg acctcttcaa
      661 gaatcttcat gacgcgttcg tgacgctttt gtttggcctg ggcccgggtc agagtttgtg
      721 tcgtagagga attgttcgac tcggtggagg aatttgcctt aaggagttcc cggcatcgct
      781 gatggacgat aaggccttct tcgatgatcg acttaatcgt cattcgggga ttgagcgagc
      841 taaaggggtc ctgaaagacg atttgcatcc ttcgccgcat tgcccgaagt tcctggccgg
      901 tcgctttaag gacgtcctgc ccgtcgaaga cgatagttcc gctggtgggt tccaacaatc
      961 taacgacagt gcggcccaag gttgtctttc cgcagcctga ttcgcccacc aggccaagcg
     1021 tttcgccccg gcgaatggcg aaggagacat catccacggc gcgggtattg gccaccacgc
     1081 gcgagagcaa accccggcgc ttggggaaat attttttcag gtgtcgaaca atcaataaga
     1141 tgtcgtgatc cataggcctt aattttgttg atcaaaaacg cagttcgccg gcgaagtgac
     1201 agcggacgaa atggcaaggt gcgatttcac gcaagtccgg ctcctcttgt tcacaaatct
     1261 tctggcgata gggacaccgc ggatggaact tgcagccgct cgggaagtct ttgggattgg
     1321 ggacgtttcc ttcgatggtg gcaagccgtg ttcgcttgtc ctgccctaaa cggggccggg
     1381 aacggaataa acctaccgtg tagggatgat acggctgttc aaaaagctct tgcaccgacg
     1441 cctgttcgac gattttggat gcgtacatga cggccacatc gtcggccaat tcggctacca
     1501 cgccgagatc atgggtgatg atcaagatgg acatttgccg ttcgtgctgg atctgcttta
     1561 gcagttccag gatttgcgcc tggatggtga catcgagggc cgtcgtaggt tcgtcggcga
     1621 ttaagagggc ggggttacag gatagggcca tggcgatcat gacccgctgt cgcatcccgc
     1681 cagagaactg atggggataa tctcgccaac ggacttcggc attgggaatg cccacctgtt
     1741 ggagcagctt gatcgtcaaa tccttagctt cgctgaagga cttcttttga tggagttgaa
     1801 ttgcctcgat gatttgcgag ccgaccgtga agacgggatt gagtgaggtc atcggttcct
     1861 gaaagatcat ggcgatatca cgaccgcgaa cagcccgcat ctcgcgatcg ctgagatcgg
     1921 ttaggacggt gggcttgttt ttggcgtccc agaatgtgat ttttcctcgc acaatttttc
     1981 cgggaggggc catcagccgc atgagtgaca aggccgtcac ggacttcccg cagccagatt
     2041 cccccaccaa gcccagggtt cggcctcgtt tgatttcgaa ggaaacgtcg tccaccgccc
     2101 ggagaatacc gtcgtcggtg aaaaaatacg tgcaaaggtt ttccacccgc aagagcggtt
     2161 gcaattcggt tgaaaggggc tgttgagttg gggcgatttg ggccgtcata agtgggcttc
     2221 agtgatcacg atgattggat ctcgtctaga ttcgtaggcg tgggtccatg gcgtcacgca
     2281 ggccgttgcc caccaggtta aaagccgtca ccgtaataaa catggccagt gagggaaaca
     2341 ccaccagcca ccagtagtga tagtttt
//
LOCUS       DAOPTR010000651         2365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_35648_length_2365_cov_3.730303, whole genome shotgun sequence.
ACCESSION   DAOPTR010000651 DAOPTR010000000
VERSION     DAOPTR010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35648_length_2365_cov_3.730303"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="PKI05_11705"
     CDS             complement(<1..921)
                     /locus_tag="PKI05_11705"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52912.1"
                     /translation="MKRNMLWIAAIAWVFSLVALARCGYTQPPWAMGGGPPGMMPGMW
                     GGPQMSQPSQASITGNPEIDRRVQFYEGLMRQMDTNQDGTVSKEEISQSRMGEYLVSR
                     MKERAGVDVSGGFRIDELRQKVIDYYKQRYGGDSQNAQSSSGSQSSTTSSQPQQSLSI
                     LRLGPNVTVPGFGTPTTSSPSSPTVPGFGSPTAGSGTPGTSSGTAPTTSTGSAPQGQV
                     LALPPPQPLDERIRRYAEGLMRQYDENRSGKLEKNEWEKMRGSDWAKADKDGNSELTL
                     QEIGEHLAAQAGGSSSSSSSSLDFLPLPLAG"
     gene            1540..1779
                     /locus_tag="PKI05_11710"
     CDS             1540..1779
                     /locus_tag="PKI05_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52913.1"
                     /translation="MRNPFTSFTRATMQSIRGNPRLRWGIERQRLQCELLEDRCLLSV
                     GPSQFELFSVMPAVFVENQGQWADASVRMSMTGTG"
     gene            1776..>2365
                     /locus_tag="PKI05_11715"
     CDS             1776..>2365
                     /locus_tag="PKI05_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52914.1"
                     /translation="MNVALTDRGPVFAVFRQGATSDNAGPEDLAGENPAEAPATEVLA
                     FSAQFVGARETVPVGRQRAETVFNYFVGPEERWRSEVPSYEVVAYEGLYEGIDLYTWG
                     QRSRLKYEFHVAPGADYRAIQVRYEGITGLSLAEDGSLIVGLGDGWGHVVDGAPYIYQ
                     EINGERLEVSGRYALVDAFTYSFELTGWYDPSRPLV"
BASE COUNT          422 a          674 c          722 g          547 t
ORIGIN      
        1 gccggccaag ggcaaaggca agaagtccaa ggacgacgaa gatgacgaag acgagccccc
       61 cgcctgggcg gccaggtgct cgccgatttc ctggagggtc aactccgaat tcccgtcctt
      121 atccgccttg gcccaatcgc tcccccgcat cttttcccat tcgttctttt ccagctttcc
      181 actgcggttt tcgtcatact gtctcataag accctcggcg taacgccgga tgcgctcatc
      241 caacggctgc ggcggtggca gggcgaggac ttggccctga ggggcacttc ccgtcgatgt
      301 tgttggcgcc gtgccggagg acgtccccgg cgttcctgaa ccggctgttg gagagccgaa
      361 cccgggaacc gtcggactgc tgggactcga cgtggtcggt gtaccgaaac cggggacggt
      421 gacgtttgga cccaagcgca aaatacttaa actctgttgg ggctgggaac ttgtcgtgga
      481 ggactggctt cccgaagagg attgtgcatt ttgggagtcg ccaccgtatc gctgcttgta
      541 gtaatcgata actttttgcc gaagctcgtc gatccggaac cctcctgaga catcgacccc
      601 agcgcgctct ttcatgcggg agacaagata ttctcccatc cgagattggc tgatttcctc
      661 cttggacact gtcccatctt ggttggtgtc catctggcgc atcagccctt cgtaaaattg
      721 gacccgccgg tcgatctcag ggttacccgt gatgctcgcc tgggatggct gggacatctg
      781 agggcctccc cacatgcccg gcatcattcc tggtggtccc ccacccatcg cccaaggagg
      841 ctgcgtatag ccgcatcggg ccagtgccac taggctaaat acccaggcga ttgccgcaat
      901 ccacaacata ttccgtttca tgagcggtcc cctacatgtc tgtttgccat ggtcggatgc
      961 aattggtcgg tcggcggcgt ccgtcccatc cccatttatt ttaccaaacg ctggcggttt
     1021 ccgcgcgcac cttcccctat aagaatgaac gatccgccgc cccagatgtt tcgccctccc
     1081 ggcaccaaaa cctcggtttg ctcgctcccc cgcggccccg ggcttccaag gaggagaatc
     1141 ctcgattcaa ccccggggct gaagatttat cttcggcatc ctgtgttgtt tctccatttg
     1201 gcccacccaa ttaaagcatt ccaggtttcc cagaccgctt tagtgatgtt ccggttcccc
     1261 tcctctccca ggcaccaaag cttattcttc gtcttcccca accgaccatc gtcctttgct
     1321 cacttgattc ccccttgtgc ccctttcaca cagccaaatg gatgcaattg acaaagagca
     1381 accccctaag acgggttatt gacaggcggg gttctatagg tagattgcag gagtcctccg
     1441 ttgcttcggg aatcagatgg aaactcgaga ttgcgttcct gagccagcgc cagggcaaaa
     1501 acaggtccct tgtcattctc ttcgtgagga gctggtacgg tgcgcaatcc attcacctca
     1561 ttcacccggg caacgatgca aagtatccgc ggtaaccccc ggttgcgctg ggggattgag
     1621 agacaacggc tccagtgtga gcttctggaa gaccgctgcc tcctctctgt ggggccctcg
     1681 cagtttgagc ttttcagtgt gatgcccgcg gtgtttgtgg agaaccaggg gcagtgggcg
     1741 gatgcttcgg tgcggatgtc catgacggga acggggtgaa tgttgcgctg accgatcgcg
     1801 ggccggtgtt tgcggtcttt cggcaggggg ccacttccga taatgcgggg ccggaagatc
     1861 tggctgggga aaatcctgcg gaagcgccgg cgacggaggt gctcgccttt tcggcccagt
     1921 ttgtgggcgc tcgagaaaca gtaccggtag ggcggcagcg tgccgagacg gttttcaact
     1981 actttgtggg tcccgaggag cggtggcgtt cggaggtgcc tagttacgag gtggtggcct
     2041 acgaggggtt gtacgagggg attgatctgt acacttgggg gcagcggagc cggctgaagt
     2101 acgagtttca tgtggcgccg ggggcggact atcgggcgat ccaggtgcgc tacgagggga
     2161 ttactgggtt gtcgttggct gaggacggtt cgctgattgt gggcctgggg gacgggtggg
     2221 gtcatgtagt ggatggggca ccgtacattt atcaggagat caacggggaa cggttggagg
     2281 tctcggggcg gtatgccctg gtggatgcct ttacgtattc gtttgagctg accggctggt
     2341 atgatccgag ccgcccgctg gtgat
//
LOCUS       DAOPTR010000652         2360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_35757_length_2360_cov_3.121475, whole genome shotgun sequence.
ACCESSION   DAOPTR010000652 DAOPTR010000000
VERSION     DAOPTR010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35757_length_2360_cov_3.121475"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="PKI05_11720"
     CDS             complement(<1..893)
                     /locus_tag="PKI05_11720"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52915.1"
                     /translation="MQSLIRRIHESPWKLVLVAAGGGIQGIADLFHEPGASRTVLEAV
                     IPYSAAALEAWLHGKPDHACAKATARSMAMAAFLRARQLQQAAGETDDSPVAGVACTA
                     SLVTERPKKGPHRVHVAIQRADVTRSVSLFLIKGARSRFEEEQVASGLFLYAMATACD
                     LEVDWALPLLSGEVLEHEKAEAPKSWQELILGTCDVVPLGAPADWQRGRPVVLFPGAF
                     NPLHVGHLRMARVAEAKLGRPVEFEISVLNADKPPLDYVEIRRRVEQFTLQDRIWLTR
                     LPTFLEKARRFPQATFVVGIDT"
     gene            1419..1808
                     /locus_tag="PKI05_11725"
     CDS             1419..1808
                     /locus_tag="PKI05_11725"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOA52916.1"
                     /translation="MGRPPVKRRRSWVEKFQAAFRGLWYGIVGQSSFAVHFAVAFLVL
                     VVAAILGVTETEWCILILSIGVVMTAELINSALEWFAPAVTQEYNRYIQTALDVASAA
                     VLLASLGAAVIGFIIFGEHLVQILVRP"
     gene            complement(1812..>2360)
                     /locus_tag="PKI05_11730"
     CDS             complement(1812..>2360)
                     /locus_tag="PKI05_11730"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOA52917.1"
                     /translation="LHQLVEQGKFRRDLFYRLNMLSFFIPPLRDRPYDIDYLARKFAV
                     EQSRSLCVRLRGIDPAFMAALHNYHWPGNVRELENIIRRAVLYCRDGILRVSDLPSMM
                     RPAPRENGGEGLPEISVRWTLDKRLEDAERRILEEALRRNGYRRQDTARELGISRITL
                     YHKMKKYGLMGNHRDEVSAVLE"
BASE COUNT          485 a          732 c          649 g          494 t
ORIGIN      
        1 gtgtcgatcc caacgacaaa ggtggcctgc gggaaccggc gggctttttc caaaaaggtg
       61 ggaagtctcg tcagccaaat gcgatcttgc aacgtgaact gttctacccg tcgccggatc
      121 tccacataat cgagtggcgg tttgtcggca ttgaggacgg agatttcgaa ctccacaggc
      181 cgccccagct tcgcttcggc gacgcgggcc atccgcaaat gtcccacgtg cagcgggtta
      241 aatgctcccg gaaaaagaac cacaggccgc cctcgctgcc aatcagccgg cgcaccgagc
      301 ggaacaacat cgcaggtgcc gaggattaac tcctgccacg acttcggggc ctccgccttc
      361 tcgtgctcca acacttcgcc ggaaaggagc gggagagccc aatccacctc caggtcacag
      421 gccgtcgcca tcgcgtaaag gaacaagcca ctggcaactt gctcctcctc gaaccgactt
      481 cgcgccccct tgataagaaa cagcgagacc gatcgcgtta cgtctgcccg ctggatagca
      541 acatgaacgc ggtgagggcc tttcttggga cgttccgtga ccagcgacgc cgtgcaagcc
      601 accccggcga ccggcgagtc gtcggtctcc cccgcggcct gttgaagttg ccgtgcccga
      661 agaaacgcgg ccatggccat gctgcgggcg gtcgccttcg cgcacgcatg gtccggctta
      721 ccgtggagcc aggcctctag cgcggccgcg gaatacggaa tgactgcctc caacaccgtc
      781 cgcgatgccc ccggctcgtg aaataaatcg gcgatgccct gaataccgcc gcctgcggcc
      841 accaggacca acttccaggg actttcatgg atcctacgaa tcagcgattg catcttagcc
      901 atcgtttcaa cggccgcctg tctatgccgg gcggcgtcaa ctaaacgatc ccacctttca
      961 ggtatcgccc cactcctcag attcagaaca aaggggggta atgagaccaa ccaaaggaac
     1021 ggtgttcgcc tctcttgctg cgattaaccc cttattctac taagagccca tcccataatc
     1081 tcccctcgcc cgctcggcgg gaaaggggtc gggggtgagg gtgactcaaa tcgctgccgg
     1141 ttttctaact cgactccgac gatcgcttgc cctcacccca accctctccc ggggggagag
     1201 ggggcttttt ttgggataga ctctaaatgg gacccacagc ggttttggca tccccttctg
     1261 attaggtcca ttagtatccc ccactcttgg gatgaacctc tggcgtcaga tgaaataagc
     1321 tacagttgga agggaaacca accttgctta attgtcggcg tcctaaagtt taggacggcg
     1381 tcaaaagtat tacctttcgc ttgatgtaag ggctaccgat gggccgtcct ccggtcaaac
     1441 gacggcgttc ctgggtggaa aagtttcagg ctgccttccg cggtctgtgg tacggcattg
     1501 tcggtcaaag cagcttcgca gtccactttg cggtcgcgtt tttggttctt gtcgtcgccg
     1561 caatcttggg cgtgaccgaa acggagtggt gcatcctcat cttgagcatc ggcgttgtca
     1621 tgacggccga gttgatcaac agtgccttgg agtggtttgc cccggccgtg acccaagagt
     1681 acaaccgtta catccagacg gcccttgacg tggccagcgc ggcggtcctc cttgcatccc
     1741 tgggggccgc cgtcatcgga tttatcatct ttggcgagca cctggtccaa atactggtaa
     1801 ggccctagct ctcattcgag cacggcggac acctcgtcgc gatgattccc catcaggcca
     1861 tatttcttca tcttgtggta cagcgtgatg cggctgatcc ctagttcacg cgccgtatcc
     1921 tgccgtctgt atccattccg gcggagcgcc tcctcgagaa tccgccgctc ggcgtcctca
     1981 aggcgtttat caagcgtcca gcggaccgaa atttcaggaa gcccctcgcc gccattttct
     2041 cttggggcgg gccgcatcat gcttggcaag tcgctgactc gcaatattcc atcccgacag
     2101 tacagcacag cccgccgaat gatgttctct agttcacgga cattccccgg ccagtggtaa
     2161 ttgtgaaggg ccgccataaa ggccggatca atcccccgca accggacaca caatgagcgg
     2221 gactgttcga cggcgaactt tcgggcaaga tagtcaatat cgtaggggcg atcgcgcagc
     2281 ggtgggatga aaaagctcaa catgttaaga cgatagaaca aatcgcgacg gaacttcccc
     2341 tgctccacga gttggtgcag
//
LOCUS       DAOPTR010000653         2358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_35793_length_2358_cov_4.137647, whole genome shotgun sequence.
ACCESSION   DAOPTR010000653 DAOPTR010000000
VERSION     DAOPTR010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35793_length_2358_cov_4.137647"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PKI05_11735"
     CDS             <1..608
                     /locus_tag="PKI05_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52918.1"
                     /translation="PRLEGIASESVVDLRQISPLWQFAQSIMWTPQSLEQYQKLAEWA
                     RIANIVKLLQIQGKIPEGVPPAVLDQAAVEAENLFKTLTPDHISQEARDRLNELATQI
                     GFQPLNGIVFVAQLIAEPSPGMVLLTFGSENQHALVRLRPDRPDVRIGAEVFVAGLVS
                     PQAAEIRIGQEGRQERVPLIWGMFLLNLEKPSQTASAEAAR"
     gene            complement(605..1390)
                     /gene="trpC"
                     /locus_tag="PKI05_11740"
     CDS             complement(605..1390)
                     /gene="trpC"
                     /locus_tag="PKI05_11740"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOA52919.1"
                     /translation="MASILDRIVSTKREEVERAKIFAPEEHLRRMVEQQPPPRDFLGA
                     IRNAPNIALIGEVKKASPSKGVIRENFDPVAIARVYEKHGATCISVLTDEPYFQGHIE
                     FLRWVKESVQIPVLRKDFILDSYQVLEARAYGADAVLLIAECLTTEQLTRLYHEILAW
                     GMTPLVEVYEPENLPRVIDLGASLIGINNRNLQTFEVDLEHTIRLRAKIPSDRLVVGE
                     SGIQTHEDVDRLRRAGVNAILVGESLMREADIGLAVDRLLGKS"
     gene            complement(1530..2318)
                     /locus_tag="PKI05_11745"
     CDS             complement(1530..2318)
                     /locus_tag="PKI05_11745"
                     /inference="COORDINATES: protein motif:HMM:NF019590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HOA52920.1"
                     /translation="MQRREFLASSMIGGVGAIMAMSELSQAVEGAGGPKQLLELRRYQ
                     IASPEKRDRLEKFFAEVAIPVWNSLGIQPVGVFRYLGEEDPSLFVLLPHNSAETVIRE
                     MAALLADPKAQSVGKDVLYDPFDDPTYQRVESSLLLAFDGVPKVQVPTTAESRVFQLR
                     IYESHNLTKHVKKVEMFNTGGELEIFYRCKMNPVFFGQALIGSRLPNLTYMLGFDNEA
                     AQKEAWAAFLKDPQWLELRDKPEYAQTVSNITNIVLRPSPVSQI"
BASE COUNT          533 a          683 c          619 g          523 t
ORIGIN      
        1 cgccacgtct ggaggggatc gcctcggagt ctgttgtcga tctgcgccag atttcaccgc
       61 tttggcagtt tgcgcagtcg attatgtgga cgccccagag cttggaacag taccaaaaac
      121 tggcggagtg ggcacggatc gccaatatcg tcaagctcct acaaattcaa gggaagattc
      181 ctgaaggtgt cccgcccgca gtgctcgatc aggcggcagt cgaggcggaa aacctcttca
      241 agacattgac accggaccac atttcccaag aagcacggga tcgtctcaat gagctggcta
      301 cgcaaatcgg ttttcaaccg cttaacggga ttgtcttcgt agcacagctc atcgcagagc
      361 catcgcctgg catggtttta ttaacgtttg gcagcgaaaa ccagcatgct cttgttcggc
      421 ttcggcccga tcgccccgat gtgcgaatcg gtgccgaagt gttcgtggcg ggcttggttt
      481 cgccgcaggc tgccgagatt cgcattggcc aagaagggcg gcaagagcgg gtgcccctta
      541 tttggggaat gttcctgcta aatctcgaaa aaccttccca aactgcgtct gccgaagcgg
      601 cacgctaact ttttccaaga agtcggtcca cggccaggcc gatgtcggct tcccgcatga
      661 ggctttcccc gaccaagatg gcgttgaccc ccgcccggcg aaggcgatcc acatcctcat
      721 gggtttggat gccactctcg cccaccacca gccgatccga aggaattttc gcccgcaagc
      781 ggatcgtgtg ttcgagatcg acctcgaacg tctgcaaatt acggttgtta atcccgataa
      841 gcgaagcccc caggtcaatc actcgcggca ggttttccgg ctcataaacc tcgaccagtg
      901 gtgtcatgcc ccaggcgaga atctcatggt aaagacgtgt caactgctcg gtagtcaagc
      961 attcagcaat gagcagaacc gcatcggcgc cgtaagcgcg ggcttcgaga acctggtaag
     1021 agtcgaggat aaaatctttc cgcagcaccg gaatctgcac gctttccttg acccaacgaa
     1081 ggaattcgat gtgcccctga aaatacggct catccgtcaa aacactgatg caggtggcac
     1141 cgtgcttttc gtagactcgg gcgatcgcga cggggtcaaa gttctcacgg atgacaccct
     1201 tggatggact ggcctttttg acttccccaa ttaaggcgat gttgggtgca ttgcggattg
     1261 cccccagaaa atcccgcggt ggtggctgtt gttccaccat gcgacggaga tgctcctcgg
     1321 gcgcaaaaat ctttgcccgc tccacttcct cgcgcttggt ggacacaata cgatcgagaa
     1381 tggaagccat gtcgagataa cctccaactt cacggcttat tggggcatcc ccctcgtgca
     1441 tgcccgcgca caacctgttg actggacacg gtcttcccac caccgcggca atatcgcccg
     1501 ttgggccatc agtcccggag cagcgattgt caaatctgtg acacgggact tggccggagc
     1561 acaatgttcg tgatgttgga taccgtttgc gcgtactccg gcttatcgcg caactcaagc
     1621 cactgcggat ctttcagaaa tgccgcccag gcttcttttt gtgcggcctc gttgtcaaaa
     1681 cccaacatgt aggtcagatt gggaagtctg gacccgatca acgcttgccc aaagaagacc
     1741 ggattcattt tgcaccgata gaaaatttcc aattccccac cggtgttaaa catctcaact
     1801 ttcttcacat gcttcgtcag attgtggctc tcgtaaatgc ggagctgaaa aacccgcgat
     1861 tccgccgtgg taggaacttg gaccttggga acgccatcga aggcaagcaa gaggctactc
     1921 tcgacccgct gataggtggg atcgtcgaac gggtcgtaca ggacatcttt gccgactgac
     1981 tgggccttgg gatcggccaa cagggccgcc atctctcgaa tcaccgtctc ggcgctgtta
     2041 tgtggcagca agacaaaaag acttggatct tcttctccca aatagcgaaa cacgccaacg
     2101 ggttgaattc ccaagctgtt ccagacggga atggcaacct cggcaaagaa tttctccaaa
     2161 cggtctcgtt tttctggaga tgcgatctga taacgacgca actcgagtaa ttgctttggc
     2221 ccaccggccc cttcaacagc ctgggacaac tcactcatgg ccatgatggc tccgactccc
     2281 ccaatcatgg acgacgccag gaactctcgc cgttgcatgg tgtaactcct cgttcaaaag
     2341 tgacctatca agatgcca
//
LOCUS       DAOPTR010000654         2352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_35899_length_2352_cov_4.046147, whole genome shotgun sequence.
ACCESSION   DAOPTR010000654 DAOPTR010000000
VERSION     DAOPTR010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35899_length_2352_cov_4.046147"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..1261)
                     /locus_tag="PKI05_11750"
     CDS             complement(191..1261)
                     /locus_tag="PKI05_11750"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOA52921.1"
                     /translation="MKSFWQIVLVGSLLTFGDLLPGKHDVFAQEVATTPEVPWTQVWT
                     QPSVVAEIETIVKAVRPEEIRRHLFYLAKDPLPFRKLNYTRPGQEKNTLYEADDYLTR
                     QLMSWGYEVRREEVRVQAFRRDPNKPKAHQYSPPASEDPWYLAYNLYAEKKGRRVPEE
                     IILLLAHKDSQSWVDSPGANDNAIGTSGLLEIARVLAQYPSDRTIRLLWCNEEHTPWT
                     SVTAAQKAKERGDKIIAVINLDGIGAKSAEDTAAGKKTNVILFTLPEGEPIAQLAFAA
                     NERFGIGLEQRIDKRPRPGDDDGSFVRAGYPAAVVMIGSYPYADPNYHTEDDIPERTD
                     VVNAAMTVQVALATVITLDRMP"
     gene            1764..1997
                     /locus_tag="PKI05_11755"
     CDS             1764..1997
                     /locus_tag="PKI05_11755"
                     /inference="COORDINATES: protein motif:HMM:NF022229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOA52922.1"
                     /translation="MSEANSSYVARIGATAGLVWHVLNENGPLNLSQIIKKVDRPRDE
                     VMQAIGWLAREDKLIFSEQGRARYVALKEKAVA"
     gene            complement(2029..>2352)
                     /locus_tag="PKI05_11760"
     CDS             complement(2029..>2352)
                     /locus_tag="PKI05_11760"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOA52923.1"
                     /translation="VPTIMVTGDEAACREARQFLGPEVVTVVVKVGLARESAMLFPFE
                     ETRQALYEGAKRAMAQIGKCKPYVIDMPIKAKKQWLVFETPDGPGKLQTKEGVIEDVR
                     DIFRF"
BASE COUNT          489 a          645 c          649 g          569 t
ORIGIN      
        1 ggggtcctgt tttttcttag ttgggcgcac cgttgtagaa gtcctcatat ccagtaacac
       61 ggtacgccct tgaccaacag acggctctcg agtgactgtc gaaggcgcat gttacgacgg
      121 ccttgctttt ttatcgcaaa cgacggttcc ttttggcccg gccgactact tctgcggcac
      181 gttcgtcgga ttacggcatt cgatccagcg tgattaccgt agccagggcc acctggactg
      241 tcatggcggc gttgacgacg tccgtgcgct ctgggatatc gtcctccgtg tggtaattgg
      301 gatcggcata cgggtaagag ccgatcatca ccacggctgc cggatatcct gctcggacga
      361 atgacccatc atcgtcgccc ggtcgaggtc gtttgtcgat ccgttgttcc agtccaatac
      421 cgaaccgttc attggcggca aatgccaact gggcgatagg ttcaccttcc ggcaaggtaa
      481 acaaaatgac gtttgtcttt ttcccggcgg ctgtgtcctc ggcagactta gcgccgatgc
      541 cgtctaggtt tatcacggca atgatcttgt cacctcgttc tttggccttt tgtgcagccg
      601 tcacgctcgt ccacggcgtg tgttcctcat tgcaccaaag aagccgtatc gttcgatcgc
      661 tgggatattg ggccaacacg cgagcgatct ccaaaagacc ggaggtaccg atggcattat
      721 cattggcacc gggtgaatct acccagctct gcgagtcttt gtgggccaag agcaggatga
      781 tctcttccgg cactcgccgg ccctttttct ccgcatacaa gttgtacgcc agataccacg
      841 ggtcctctga cgccggtggc gaatactggt gggccttggg cttattgggg tcccgacgaa
      901 acgcctgcac gcgcacctcc tcccgccgga cctcatagcc ccacgacatc agttgccgcg
      961 tcaaataatc gtcggcctcg tagagggtgt tcttttcctg accgggccgg gtgtaattga
     1021 gttttcggaa cggcaaaggg tccttggcca ggtaaaagag atggcggcgg atttcctctg
     1081 gccgaaccgc cttcacgatg gtctcgattt ccgcaaccac actgggttgg gtccatactt
     1141 gtgtccaggg gacctcgggc gtcgtagcga cttcctgggc gaacacgtcg tgtttccccg
     1201 gaaggaggtc gccaaaagtc agaaggctcc caaccaaaac gatctgccag aacgatttca
     1261 cacgtcgtcc tccctaggtt actgggttga taaaatcccc gctctcacgg tacacaaatg
     1321 cgctgacact tcgctttgcc tacgctcgtc cggcttttcc atgatactgc cgacttctgg
     1381 aaaagaaacc ccgcccgata ccattgccaa gagtagagag caaaggacgt cgtgtccttt
     1441 caagcggctt ttgcacgaat gggccgatct ttgcaaatcg ccttcgttac aagatggcca
     1501 aagctcagtg tgttgagagt tccgcgccga ggtggaagat cgagctcaat tagcctccga
     1561 acggcgggca gctccagtgc cttcttggaa aaggtggcag atgttcgtaa aatgcgatca
     1621 agggaacata ggtacacggt gaagccgacc gtgcgagtcg gaccttttga gatgttggtt
     1681 ggtcatgagg ggtgattttt ccgaaacggg caaggatggg agtttctggc gtgttttaat
     1741 gttttgaaca aagggatgga gccatgagtg aagcgaattc ttcttacgtg gcgaggatcg
     1801 gagcgaccgc cggtttggtg tggcacgtcc tgaatgagaa cggtccactt aatttgagtc
     1861 aaattatcaa aaaagtggat cggccgcgag acgaagttat gcaggccatt ggttggttgg
     1921 cccgggaaga taaactcatc ttttccgagc aggggcgtgc ccgttatgtc gccctgaagg
     1981 agaaggccgt cgcctgacgc cccgcggtct tttcgccaaa actcatcatc agaatcggaa
     2041 gatatcccgc acgtcttcga tcacgccttc ctttgtctga agttttcccg gtccgtcagg
     2101 cgtttcaaag acaagccact gctttttcgc tttgatgggc atgtcaatga catagggctt
     2161 gcatttgcca atttgagcca tagcccgttt tgcaccctca taaagggcct gacgcgtttc
     2221 ttcaaaggga aacagcattg ccgattcgcg ggctaaaccc actttgacaa ccacggtaac
     2281 cacttcggga ccgagaaact ggcgggcttc ccgacatgcc gcttcgtcac cggtcaccat
     2341 gatcgtcggc ac
//
LOCUS       DAOPTR010000655         2351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_35915_length_2351_cov_3.298345, whole genome shotgun sequence.
ACCESSION   DAOPTR010000655 DAOPTR010000000
VERSION     DAOPTR010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35915_length_2351_cov_3.298345"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..1189
                     /gene="gmd"
                     /locus_tag="PKI05_11765"
     CDS             197..1189
                     /gene="gmd"
                     /locus_tag="PKI05_11765"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007330038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOA52924.1"
                     /translation="MTKRALITGITGQDGSYLAEFLLSKRYEVHGMVRRASTEGFERI
                     QHIRDQLHLHQADLLDQLSLVRLVAEVRPHEVYNLAAMSFVPTSWQQPLLTGEFTALG
                     VTRMLEAIRHVDPTIRFYQASSSEMFGAVREEPQNENTPFYPRSPYGVAKVYGHWITV
                     NYRESFGVFACCGILFNHESPRRGKEFVTRKVTDAVARIKLGVQDKLYLGNLDAMRDW
                     GFAGDYVEAMWLMLQQPKPDDYVIATGEKHSVRELVELAFDYAGLDWKRYVEIDPKLI
                     RPAEVYTLRGDASKARRVLQWSPKVSFPELVKMMVDADLERVRREIADRAHRLP"
     gene            1259..>2351
                     /locus_tag="PKI05_11770"
     CDS             1259..>2351
                     /locus_tag="PKI05_11770"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOA52925.1"
                     /translation="MRAHSRFKRTCVQLLKIAASVAILAWLVWDSVRRGEGLFSDEHG
                     FQPEKLWALVRHAVDNWLILLLAFICAFASVMITFIRWWLLIRSVGVPISLRESLRFS
                     FVGYLLNLAPMGIVGGDLAKAVLVARHCPEHRAPSVASVFVDRVVGLYVLLLVASGAI
                     LLTNFHATGPAIVQSACRLTLVVSVLGTIVLLLVWAPDWTGGRVRRLWEKLPVVGRPI
                     ARFTEAFRAYGREPGTLLLSLVMTLPVHLLFAIVVFLVGTALFPKVHSLDRHFVFAPL
                     AGVMQVLPISVGPAEFVLDRLFVLTPTADGSIIPPGQGLVTLLVYRVFNLLISTVGVV
                     YFLMSREEWREALHEVEEVEEPPSDLEIEV"
BASE COUNT          454 a          616 c          691 g          590 t
ORIGIN      
        1 cgcggccggg ttggcactag acttttctcc gtcaaacaac atcgcgtgcc gctcagaggt
       61 tatcatggaa agatagggga cgtggtggaa catccgctga cattaacaca cccagcgaaa
      121 ctggggacaa cgcagcggtt gtgggtcaat tatctgaatc attgaactgt caatcttttg
      181 aaaagatctt gggcttatga ccaaaagggc gctcattacg gggatcacag gacaggacgg
      241 ttcttacttg gccgagttcc tgttgagtaa aaggtacgag gttcatggga tggttcgtcg
      301 ggcgagcacg gagggcttcg aacgcatcca gcatattcgg gatcaacttc acctccatca
      361 ggccgatctc ttggatcaac tttccctggt gcgacttgtc gcggaggttc ggcctcatga
      421 ggtgtataac ctggcggcca tgagctttgt gcccaccagt tggcaacagc ctttgctgac
      481 gggagagttc acggccttgg gcgtcactcg gatgcttgag gccattcgcc acgttgatcc
      541 gacaattcgc ttttatcagg ccagttctag cgaaatgttt ggggcggtcc gcgaggaacc
      601 tcagaacgag aacaccccat tttatccccg cagcccttac ggcgtggcca aggtctatgg
      661 gcattggatc acggtcaact atcgcgagag tttcggtgtc ttcgcatgct gtgggattct
      721 gtttaatcac gagtcgccgc ggcgtggcaa ggaattcgtt acccgcaagg tgaccgacgc
      781 tgtcgcgcga atcaagttag gagtccaaga taaactctat ctcggcaatc tggacgccat
      841 gcgggattgg ggatttgccg gtgattatgt ggaggctatg tggctgatgc ttcaacagcc
      901 gaagccggat gattatgtga tcgccaccgg agaaaaacac tccgtgcggg aacttgtcga
      961 actagcattt gactatgccg gactcgattg gaaacggtat gtcgagattg atccgaaatt
     1021 gattcgcccg gcggaggttt ataccctgcg cggcgacgcc tcaaaagccc ggcgtgtgct
     1081 tcagtggtct ccgaaggtct ccttccctga attagtcaaa atgatggtgg atgccgattt
     1141 ggaacgggtc cggcgcgaga ttgccgatcg ggcacacaga cttccgtaaa ggctgctcgg
     1201 caacatcaac aaagctgtaa caagaagatt gtttcgcccc gcagggggct tgtgttttat
     1261 gcgagcacat tcgcgtttca aaaggacgtg tgtccaatta ttaaagatcg ccgcttcggt
     1321 ggctatcctg gcgtggcttg tttgggatag tgtccgccgc ggggaggggc ttttctccga
     1381 tgagcatggc tttcagccgg aaaaactttg ggcactcgtc cgccatgccg tggataattg
     1441 gttaatcctc ctcctggcct tcatttgtgc tttcgccagc gtcatgatca cattcattcg
     1501 ctggtggcta ttgattcggt cagtcggggt acctatcagt cttcgcgaat cgctccgatt
     1561 tagtttcgtt gggtaccttc tcaatctggc cccgatgggc attgtggggg gagatctagc
     1621 caaggcggta ctggtcgcgc gacattgtcc tgagcaccga gccccgtctg tggcctcggt
     1681 cttcgtcgat cgtgtcgtgg gattatacgt gttgcttctc gtggcgtctg gggccatcct
     1741 actgacgaac ttccatgcca cgggaccagc gatcgtccaa agcgcatgtc ggttgaccct
     1801 ggtggtcagt gtgctgggga ccattgtgct cttattggtt tgggcacccg actggaccgg
     1861 cgggagagtt cgtcgtctct gggagaaatt acctgttgtc gggcgaccga tcgcccgatt
     1921 caccgaggcc tttcgggcct acggccggga accaggcacg ttgctccttt cgctggtcat
     1981 gacgctcccc gtgcacctcc tatttgccat tgtggtgttt ctcgtgggaa cggccctgtt
     2041 tccgaaagtc cacagcctcg accggcattt cgtcttcgcc cccctggcag gggtcatgca
     2101 ggtcctgccg atctccgtgg gaccagccga attcgttctc gatcgcttgt tcgtcctaac
     2161 accgacggcc gacggcagca taatcccccc cggtcagggg ctggtcacgc tcctggttta
     2221 tcgtgtcttc aatttgttga tctccactgt gggggttgtt tattttctga tgtcgcggga
     2281 ggaatggcgc gaggcccttc acgaggtgga agaagtagaa gaacccccga gcgatttgga
     2341 aatcgaggtc c
//
LOCUS       DAOPTR010000656         2351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_35917_length_2351_cov_3.203397, whole genome shotgun sequence.
ACCESSION   DAOPTR010000656 DAOPTR010000000
VERSION     DAOPTR010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35917_length_2351_cov_3.203397"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..2105
                     /locus_tag="PKI05_11775"
     CDS             204..2105
                     /locus_tag="PKI05_11775"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA52926.1"
                     /translation="MYRHVFGSLGAILALWFGLTLASHRTIWAEQARPNILWLTCEDM
                     GPHLGCYGDRYANTPNLDRLAAKGTIYTRVWSNAPVCAPARTAIISGMYPSTTGSEHM
                     RSLVPTAPGLRFYPQFLRDAGYYCTNNSKEDYNLEKPGQVWDDSSGKAHWKNRQPGQP
                     FFAIFNFTVTHESQVRRRPHEWVHDPAKVRVPAYHPDTIEVRQDWAQYYDNITTMDRM
                     AGEKLKELEDAGLADDTIVFFYGDHGPGLPRCKRCPYNSGLQVGLIVYIPPKFRHLAP
                     PDYQPGGRSSRLVSFVDLGPTLLSLVGIKPPASMQGRAFLGTFATEPPRYMFGLRGRM
                     DERYDLVRSVTDGRFVYIRNYMPHLPWGQHVEYLFQTPTTRVWKQLFDEGKLSPEQSY
                     FWTIPKAKEELYDLATDPDEVHNVANNPAYKAKLEELRQVLRDHILTTRDLGFLPEDE
                     IHARAGSKTPYEYGQSADYPLERILTMAEVASDLEEDHLEQLLAGLQDPDSAVRYWAV
                     TGLLARGEKVFSAVHDRLVKALEDPAPSVQIVAAWTLGRYGSQQDVELALSVLRTYLD
                     YEGKGPYLMMLALNAVDNLGTKAAPLREIVAQIEPKIQPKHGRLGENLNKLKRHILSH
                     FEVAGKEQP"
BASE COUNT          517 a          674 c          634 g          526 t
ORIGIN      
        1 caagaaaagg tcccacaacc cacacaccct gcggctaagg cttttttaac acccgtggta
       61 aaacccaaaa acacgggatt ccagaacaac gcaggcggct caaccttttg ggctcggtgt
      121 tgcattttct gcggcaacgg ttcacactag atgggcaacc ggtcatttag cctcgtgaac
      181 gtcctttttg gggaggtcac aagatgtatc ggcatgtgtt tggttctctg ggagcgatcc
      241 ttgctctttg gttcggtctg actttggcgt cccaccgcac catctgggct gaacaagctc
      301 gcccaaacat cctttggctc acctgcgagg atatgggccc gcacctcggc tgttacgggg
      361 atcgttacgc caacacccca aatctcgatc gtctagcggc taaaggcacg atttacacga
      421 gggtttggtc caacgcccct gtgtgtgcgc cggcccgcac ggccatcatc tcggggatgt
      481 atcccagcac aacaggttca gaacacatgc ggagcctggt gcccaccgcc cctggcttaa
      541 ggttttatcc tcagttcttg cgcgacgccg gttactactg caccaataac agtaaggaag
      601 attacaacct cgagaaacct ggccaagtct gggatgactc gtccggcaag gcgcactgga
      661 agaaccgcca acccggccag cctttttttg ccatttttaa ctttacggtc acacacgaaa
      721 gccaagtgcg tcgtcggcct cacgagtggg tccacgatcc ggcgaaggtt cgagtcccgg
      781 cttatcaccc cgatacgatt gaggtccgcc aggattgggc ccagtactat gataacatca
      841 cgacaatgga tcgcatggcg ggagagaagc tcaaggagct tgaagacgca ggcctggccg
      901 acgataccat cgtgttcttc tacggtgacc acggccctgg tctgccccgt tgcaaacgct
      961 gcccgtataa ctctggcctt caagtcgggc ttatcgtgta tattcctccc aagtttcggc
     1021 atttggcacc tccggactat caacccggcg gccggtcgtc gcgattggtg tctttcgtgg
     1081 acttggggcc cacgttgctc agtctcgtcg ggatcaagcc gccggcctca atgcaggggc
     1141 gggcgttcct tgggaccttt gccacggagc cgccgcgtta tatgtttggc ctccgcggtc
     1201 ggatggacga acgatatgat ctcgtccgca gcgtgaccga tggtcgcttt gtctatatcc
     1261 gcaattacat gccgcacctt ccttggggtc agcatgtcga gtatctcttt caaaccccta
     1321 cgacacgcgt gtggaagcaa cttttcgacg aaggaaaact gtcgcccgaa cagtcctact
     1381 tttggacaat tcccaaggcc aaggaggaac tgtatgacct ggccaccgat ccggacgaag
     1441 ttcataacgt ggccaacaac ccggcttaca aggcaaagct tgaggagctc cgccaggtcc
     1501 tgcgggatca tatcctgacc acgcgcgatt tgggattcct tcctgaggac gaaatccacg
     1561 ctcgggccgg cagcaagacg ccctacgaat acggtcaaag tgccgattat ccgctagaac
     1621 gtatcctcac catggcagaa gtcgcctcag acttggaaga ggaccatctc gaacaattgc
     1681 tcgccgggtt acaggacccc gatagcgcgg tgcgatattg ggccgtcacc ggtctgttgg
     1741 cgaggggaga aaaggtcttt tcggcggtcc atgatcgcct tgtcaaagcc ctggaagatc
     1801 cggcgccgag cgtccagatt gttgctgcgt ggacactggg ccggtatgga agtcaacaag
     1861 atgtcgagct tgccctatct gtgctacgga cctatctcga ttacgagggg aaagggccct
     1921 atcttatgat gctagcactg aacgccgtgg ataacctagg aaccaaggct gccccgcttc
     1981 gtgagatcgt ggcccaaatc gagccgaaga ttcagcccaa gcacgggcga cttggagaga
     2041 atcttaacaa gttaaagcgg catatcctca gccattttga ggttgctggg aaagaacagc
     2101 cataaacgac catcctcaga aaacaacatt gatgggacgg catggtgaat aggttatgct
     2161 gtcgcgcgtt attttggaat cagattgttg ctcgtatagc attcacttga ttcttgtgtc
     2221 ccattcgagc caccatgacc tgaccgcgtc aacacacccg tgggtatcct cactggggtt
     2281 gattccctca agggtcaatg gtctctctgg ctaggcgagc gccgtgcagt tgctcaactc
     2341 ctgcactgtc a
//
LOCUS       DAOPTR010000657         2346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_36025_length_2346_cov_4.091663, whole genome shotgun sequence.
ACCESSION   DAOPTR010000657 DAOPTR010000000
VERSION     DAOPTR010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36025_length_2346_cov_4.091663"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PKI05_11780"
     CDS             complement(<1..499)
                     /locus_tag="PKI05_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52927.1"
                     /translation="MEVAINREMSLFVRTLIGLVLLLAGSFEPEVLQAGLGPENVLLV
                     VNEKSAASLTVANHYQALRQIPSENVFYLSWDPKEQITDIDRFREKILIPILQEIAKR
                     AVTAQIDCVLYSADFPWGIELAKDIERLIEEHPELLPPPKRTEASAEEEKESPGDRWP
                     RVLTRR"
     gene            975..1628
                     /locus_tag="PKI05_11785"
     CDS             975..1628
                     /locus_tag="PKI05_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOA52928.1"
                     /translation="MRLLTCIVALFLSGPVLAGEPSGCLTPEEQEQGFVCLFDGTSLE
                     NWQGDTKGYVIEDGVLVCKPGGNLYTKKEYADFIFRFEFKLTPNANNGVGIRTPMGCD
                     AAYCGMEIQILDDSGERYENLQPYQYHGSIYGVVPAKRGHLKPVGEWNSEEIYCKGRH
                     VKVTLNGVVIVDANLDEVEPMDHRDHPGLKREKGYIGFLGHGTRVEFRNIRIKELTP"
     gene            1662..>2346
                     /locus_tag="PKI05_11790"
     CDS             1662..>2346
                     /locus_tag="PKI05_11790"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOA52929.1"
                     /translation="MRYLGIEIGGTKLQVGVGTGNGPPLLAIRRMEIDRSKGAEAIRR
                     HILELARPLIAQYHPEAIGIAFGGPIDAAKGRTLRSHHVAGWEEFPLVEWCGSELGLP
                     AVMGNDADLAGWAEATFGAGKGHRTVFYITVGTGIGGALIHEGKIYTGAHGIAAEIGH
                     LRPGLDAYSPKDDLESISAGWGIAAQAEAFINWFLEQRAAKSPGEHIVQMARARLGLS
                     QVESVSFEEL"
BASE COUNT          496 a          592 c          685 g          573 t
ORIGIN      
        1 ctcgccgcgt taacacccga ggccaacgat cgccagggga ctctttttcc tcctcggcgg
       61 atgcctcggt gcgtttgggc ggcgggagga gctccgggtg ttcttcaatg aggcgttcaa
      121 tatcctttgc caattcgatc ccccatggga agtcggccga atacagcacg caatcaatct
      181 gtgctgttac ggctcttttc gcaatttcct gcaaaatggg aatgaggatt ttttcgcgaa
      241 agcggtcgat gtccgtgatc tgttccttcg ggtcccacga gagataaaag acgttttcgg
      301 aagggatttg acgcaacgcc tgatagtggt tggcgacagt caatgatgcc gcgctttttt
      361 cgttgacgac taagagaaca ttttcgggcc caagcccagc ctgcaatact tcgggctcaa
      421 aactgcccgc taagagtaag accaggccga tgagcgtgcg aacaaaaagg gacatctcgc
      481 gattgatggc aacctccatt atgttccttt ctttggagaa cgtatgtatc cctacctgac
      541 cggggcgttt taatgatcga gtccctttgg gccgtaagcc acaggcctcc tgcgagattg
      601 cgctgctacc agaggccgat tgcattagag cctatcccat aatctcccct cgcccgttcg
      661 gcgggagagg ggtcgggggt gagggtggct caaattgctg ccgcttttgt caatcgattc
      721 cgatgttcgt ttaccctcac tccaaccctc tcccagaggg agagggcgac tttttgagat
      781 aggctgttag taaagggatt accgccatcg ccacctgttt cacgcggcct ttctgccaaa
      841 gcgttttcac ttggcaacag ttgcccccaa gtgtcatctg cgatcgggtt gattatactc
      901 atagcaacgt gtagctggtt tcccgtcaag gcgggtattc tttgctgaat ggttcccgtg
      961 taaggagttg cctcatgaga ctgctgacct gtattgtcgc gcttttcctc agtggaccag
     1021 tgctggcggg cgaaccctca ggatgcttga cgcccgagga acaagagcaa ggatttgttt
     1081 gcctcttcga cggcacctcg ctcgaaaatt ggcaggggga taccaagggc tatgtgattg
     1141 aggatggcgt tttggtgtgc aagcctggcg gaaatcttta tacgaaaaag gaatacgccg
     1201 attttatctt tcgttttgag ttcaaattga cccccaatgc aaacaacggc gtgggcattc
     1261 gcactccgat gggttgcgat gcggcgtatt gcgggatgga aattcagata ctcgacgatt
     1321 cgggtgagcg atatgagaat ttgcaaccct atcagtatca tggatcgatt tacggggttg
     1381 ttccagctaa gagaggtcac ctcaagccgg tcggggagtg gaacagcgag gaaatctatt
     1441 gcaagggtcg gcatgtcaag gtcaccctca acggagtagt catcgtcgat gccaacctcg
     1501 atgaggtgga gccgatggac catcgcgatc atcccggctt gaagcgtgaa aagggttaca
     1561 tcggattctt ggggcatggc acccgggtcg aattccgtaa catccgcatc aaggaactca
     1621 caccatagcc aaaatggcac tattggcctt ggtttgtatc gatgagatac ctaggcatcg
     1681 agattggcgg tacgaagctg caagtcggcg tcggcacggg caatgggccg ccgctgttgg
     1741 caatccgtcg catggagatc gatcggtcaa aaggtgccga ggccattcgt cggcacatcc
     1801 ttgagcttgc caggcctttg atcgctcaat atcatcctga ggcgattggg attgcttttg
     1861 gcggccccat cgatgccgcg aagggacgca ccttgcgcag tcaccacgtg gccgggtggg
     1921 aagagtttcc tttggtcgaa tggtgtggtt cggaattggg cctcccggct gttatgggaa
     1981 acgatgccga tttggcaggt tgggccgaag ccacattcgg ggcaggaaaa ggtcatcgta
     2041 ccgtctttta cattacggtc gggaccggca tcggcggtgc cttgattcac gagggcaaga
     2101 tttataccgg tgcccacgga atcgcggcgg aaatcggtca tctccgcccg ggattggacg
     2161 catattctcc gaaagacgac cttgaatcga tttcagcggg ttggggtatt gccgcgcagg
     2221 ccgaggcttt tatcaattgg tttctcgaac aacgcgccgc caagtcccct ggtgagcaca
     2281 tcgttcagat ggcccgggct cgcttgggac tttctcaagt ggagtcggtt tcgtttgaag
     2341 agctgg
//
LOCUS       DAOPTR010000658         2346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_36032_length_2346_cov_2.772588, whole genome shotgun sequence.
ACCESSION   DAOPTR010000658 DAOPTR010000000
VERSION     DAOPTR010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36032_length_2346_cov_2.772588"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PKI05_11795"
     CDS             complement(<1..412)
                     /locus_tag="PKI05_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOA52930.1"
                     /translation="MGSAMCGHLLKGGYRCTVYNRSRSKAEALLAAGATWADSPKRVA
                     EVSDIVFTIVGYPNDVREVILGPDGVLAGLPTNGVVVDMTTSEPSLAIEIAKAAAARG
                     CHALDAPVSGGDVGAREARLSIMIGGEPDIVAAVM"
     gene            complement(590..1240)
                     /locus_tag="PKI05_11800"
     CDS             complement(590..1240)
                     /locus_tag="PKI05_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52931.1"
                     /translation="MLVFLGVFQLNLPVADATQLLVALAIIIFSILGSLLQSRQEAKK
                     RQERMERPRGRGRGPDTIEEMLRESSRPTPSRPQKRPSPPKPPPVVEAEVVETSIAEH
                     VTQTFQTTMARGEPVAGEPGSLAREKRAERKASRRPRLETHPEIPGTVKEGLPELVEP
                     TATANQISSEVTSSDLMATSASPVMEGLANMLNSPEGLATAIVLKEILDRPEARWH"
     gene            complement(1285..2316)
                     /gene="floA"
                     /locus_tag="PKI05_11805"
     CDS             complement(1285..2316)
                     /gene="floA"
                     /locus_tag="PKI05_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327694.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOA52932.1"
                     /translation="MIGFVLVLAAIIVGLIFLAVFFKFFRLWIQSKTTGARIGILDLL
                     GMTFRKVRPEVIVRAKIMAVQAGITEQDGVTTRALEAHYLAGGDVNKVIRAMIAARNA
                     KMTELDFRLATAIDLAGRDVLEAVQTSVYPKVIDCPKKGSGKESLEAVARNGIQLKVQ
                     ARVTVRANILQLIGGATEETIIARVGEGIVSAIGSAESHYEVLENPDRISKTVLARRL
                     DRQTAFEIVSIDIADIDVGQNIGARIQADQAEADMRVAQAKAEGRRAMAVAAEQENLA
                     LIEEARATLVKAEAEVPRAIAEAFETGKLGIMDYYRLKNIQADTDMRTAISRMGLPLP
                     QQGRTPQAG"
BASE COUNT          519 a          708 c          594 g          525 t
ORIGIN      
        1 gcatcaccgc cgcgacaatg tccggctcac cgccgatcat aattgaaagc cgtgcctctc
       61 gcgcacccac gtcaccacct gacacaggtg cgtccaacgc atgacatccc cgcgccgcag
      121 cggctttagc gatttctatg gccaaacttg gttcactggt tgtcatatcg acgacgacgc
      181 cgttagtggg caatcctgcg aggaccccgt ccggccctaa gatgacctcg cgaacatcat
      241 taggatatcc gactatcgtg aatacgatgt ctgagacctc ggcaacacgt ttcggagaat
      301 cggcccaggt cgcaccggcg gcaagcaatg cttccgcctt gctccgactg cgattataga
      361 ccgtacatcg ataccccccc ttgaggaggt gtccacacat cgcggagccc atgactccgg
      421 ttccaatcca gccgatacgc gttctatcag gattcgcaat aggcaccgac atcgttacct
      481 ccaatgcaaa ctgactgacg gactctattg gcatggccta ctactggcta gcgttgtagg
      541 caaccttagt cttagccgaa ggccaaactg acaaaaggaa catccgactt tagtgccacc
      601 gagcctcggg ccgatcgagg atctctttga ggacaatcgc cgtagcaagg ccttcgggtg
      661 aattcaacat gttcgcgaga ccctccatca cgggcgaggc cgaagtggcc atcaaatcgg
      721 aggaggtgac ctctgaggaa atttgatttg ctgttgctgt tggttcgacg agttcgggta
      781 gtccctcctt cacggtgccc ggaatttccg ggtgtgtttc aaggcgaggt cggcgacttg
      841 ccttgcgttc ggcccgcttt tcgcgagcta acgaacctgg ctcaccggca acgggctccc
      901 cacgcgccat ggttgtttgg aaggtttgcg tgacatgttc agcaatgctt gtctcgacga
      961 cttcagcctc aacaacaggc ggaggtttcg ggggggacgg tcttttctgc ggacgagaag
     1021 gtgttggccg actggattct cgcaacatct cctcgattgt atcggggcca cgaccacgac
     1081 ctcgcggtcg ctccatgcgc tcctgtctct tttttgcttc ttgccggctt tgcagcaaag
     1141 agccaaggat agaaaagata ataatggcga gggcgacaag cagttgcgta gcatcagcaa
     1201 ccggcagatt cagttgaaaa acgcccaaaa aaaccaacat cggaacatgc ctcccataaa
     1261 aggacgccat caaccgtcaa tcatttatcc cgcttgcggt gttcgccctt gctgcggcaa
     1321 aggaagcccc atacgggaga ttgcggttct catatcggtg tcagcttgaa tgttcttcag
     1381 acggtaataa tccataatgc cgagcttgcc cgtctcaaac gcttcggcga tcgcgcgggg
     1441 gacctcggcc tccgccttca ccagcgtagc acgggcctct tcgatcaagg ccaagttttc
     1501 ctgttccgct gccacggcca tggcacgtct tccctcagct ttggcctggg ccacccgcat
     1561 atccgcctcg gcttgatcgg cctgaatacg ggcgccgata ttttgtccta cgtcaatgtc
     1621 ggcaatatca atcgagacaa tctcgaacgc cgtttgccgg tcaagacgcc gggccaaaac
     1681 ggtcttagaa atccggtcag ggttctcgag gacctcgtaa tggctctcgg ctgaaccgat
     1741 ggcactgaca attccctccc ctacccgagc gatgatggtc tcttccgttg cacctccgat
     1801 gagctgcaga atattggcac gaaccgttac ccgggcctgc accttgagct ggataccgtt
     1861 acgtgctact gcctccagcg attctttacc agaacccttc ttaggacagt caatcacctt
     1921 cggatacacg cttgtttgaa cggcttccaa gacgtcccga cccgcaaggt caatggccgt
     1981 ggccagtcga aaatcgagct ccgtcatttt ggcattcctg gccgcaatca tcgcccgaat
     2041 gactttgttg acatctccac cggccagata atgcgcttcc aaggcccgtg tggtgacgcc
     2101 atcctgttcc gtaattcctg cctgaacggc catgatcttg gcccgtacga tcacctcagg
     2161 ccggaccttg cggaacgtca ttcccaataa gtccaaaatt ccgatgcgtg ctcccgtcgt
     2221 ctttgactga atccacaaac ggaagaactt gaaaaacacg gccagaaaga taagcccaac
     2281 aatgattgca gcaagaacca gaacgaaccc aataatctgg acaaagtcgt tttgcccttg
     2341 ttgtgc
//
LOCUS       DAOPTR010000659         2345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_36047_length_2345_cov_3.868559, whole genome shotgun sequence.
ACCESSION   DAOPTR010000659 DAOPTR010000000
VERSION     DAOPTR010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36047_length_2345_cov_3.868559"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..880)
                     /locus_tag="PKI05_11810"
     CDS             complement(167..880)
                     /locus_tag="PKI05_11810"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOA52933.1"
                     /translation="MTQPCERRAFLHQVGLGSAMIGAGLGGSLLAEEAESQRQSKTVK
                     IVAVSCSPRKGKTTAQGLKIVLDAAKAVAPEKIETVLIELADLRIPVEPALGIPLPPG
                     ERDDFPAVAEQIADPRLKALIVGSPVHYANMSTLCKAFIDRLGQVFRKEFKLSGKLAG
                     ALAVGAARNGGQEYVLDSIHRALMCHEMAIVGDGRPTGHFGATLWNNGGNDDISTDEF
                     GVKTAQNLGRHIAELALKL"
     gene            1530..>2345
                     /locus_tag="PKI05_11815"
     CDS             1530..>2345
                     /locus_tag="PKI05_11815"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOA52934.1"
                     /translation="MDPLDQTRKCLRYATRKDPRSPKNQLSCLSHKWGLGSLVCFCTL
                     VALLLATGCRTMDFYDRGLEQPAPTQELEPPRELSKVSLPSYRLEPPDVVQIEVPKMV
                     PLPPYRLEVYDVLQVDVIGTLLDQPINGYYMVEAEGTINLGPAYGSVRVVGMTVAEAR
                     WAIEAHLRQILRAPEVSVQLVRASGLQPISGVYLVGPDGTINLRQYGAVYVAGLTIPE
                     AREAVRRHLSQFLDSPEVSLDVLAYNSKVYYVITEGPGEDDSVIRLPCTGNETV"
BASE COUNT          499 a          713 c          624 g          509 t
ORIGIN      
        1 cttcccctcg cgaagtgctc cccagaaaac tcgccagacc ggcagttccc aatccaatcg
       61 cggcctgatg aaattcgcgg cgtttcataa ataaccaacc tcgctgtgaa atcccgtcac
      121 agtctcgtaa agatttgacc tcagtttaag agctggtagg ggcgacttat agctttagag
      181 ccaactcggc gatgtggcgg cccagattct gggcagtttt aacaccgaat tcgtcggtag
      241 atatgtcgtc atttccgcca ttgttccaca atgtagcccc aaagtgacct gtcggacgac
      301 catcacccac gattgccatt tcatgacaca tgagcgcccg atggatcgaa tccaacacat
      361 attcttggcc accgttgcgg gcagccccta ccgccaaagc gcccgccagt tttccagaca
      421 gcttgaactc ctttcggaac acctggccga gacgatcgat gaaagcctta cacagcgtac
      481 tcatgttagc gtagtgtaca ggcgaaccta caatgagtgc cttcaaccgc ggatcggcaa
      541 tctgctcggc caccgccgga aaatcgtcgc gctcgccagg cggcaagggg atccccaatg
      601 cgggctctac ggggatgcgc aaatcggcca gttcaatgag gaccgtctca atcttttctg
      661 gagccaccgc tttcgcggca tccaacacaa tcttcagtcc ttgagcggtg gttttgcctt
      721 tccgtgggct gcaggacacc gcaacaattt tcaccgtctt cgattgacgt tgggactcag
      781 cttcctccgc cagcagagat ccccctaagc cagccccaat catagcagaa cctaaaccaa
      841 cctgatgcaa aaaagcgcgt cgttcacatg gctgtgtcat gtcgtctctc cttcggaagc
      901 gtacaaataa gccactggtc tttaacaacg agaacaccgt gcgacgcacg gaccgtcccc
      961 ccttcgtcgg aacacgcagc attttagtca gcgcactgac aattgcaaca gtggtcccat
     1021 tggagagacg cccgctccag agaaaaccca catactgtcg tggcgtgcta tcctggtagt
     1081 ccaggcgctg tcgtggccgc accaaatgac gcggtaaccg ttcctgccac atggaaccta
     1141 acggtgtgcg tcgcttagcg ttcggtttcc ctccggaaac ggagctattt cccgaatgcc
     1201 aaaatgacgt ggtaaactgc cgacggtcca gatctcttcc aaatcagccg tgcccggggc
     1261 ggtcactcgc gaaaatctct ggaaggcttt cctgtcaggc tttgtcgaag gggcctactc
     1321 ggggtagaca ttacgaccat gttgcggcca ttccacccaa attgcgggcg gctaatagaa
     1381 catctggggg cattttcttt tttcaccaag tttccggtaa actttgccga ttattccgac
     1441 agcggcgaat gcagagagag tatcgcttaa tccggttgta cgggttcagt gagccgttcc
     1501 gctggaactc aaaaggatgt tacccgacca tggaccccct ggatcagacc agaaaatgcc
     1561 tgcgatacgc taccagaaaa gatccccgct ccccgaagaa tcaattatca tgtttatcgc
     1621 ataagtgggg attgggatcg cttgtctgtt tctgcaccct cgtggcgctc cttctcgcca
     1681 cgggctgtcg caccatggac ttctacgacc gtgggctgga acagccggcc cctacccagg
     1741 aattggaacc tccgcgagag ctgtccaagg tctccctccc ctcctatcgc ctggaaccgc
     1801 ccgatgtcgt ccagatcgag gtccccaaga tggtcccctt gcctccctat cgccttgagg
     1861 tctatgacgt cctccaggtc gatgtcatcg ggacactcct cgatcagccg atcaatgggt
     1921 actacatggt ggaggccgag ggcacgatca acctcggccc ggcctatggc tctgtgcgtg
     1981 tggtggggat gaccgttgcc gaggcccgct gggccattga ggcccatctg cggcagatcc
     2041 tgcgtgcccc agaggtctcg gtgcaactgg tgcgggccag tggtttgcag ccgatcagtg
     2101 gggtctatct ggtgggacct gacgggacga tcaacctccg gcagtacggg gcggtctatg
     2161 tggcggggct gacgatcccc gaggcccgcg aggccgtccg gcgccacctc agtcagtttc
     2221 tggattctcc cgaggtctct ctcgacgttc tggcctataa cagcaaggtc tattacgtga
     2281 tcacagaggg gccgggagag gacgacagcg tgatccgcct gccttgcacc gggaacgaga
     2341 cggtg
//
LOCUS       DAOPTR010000660         2344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_36078_length_2344_cov_3.768021, whole genome shotgun sequence.
ACCESSION   DAOPTR010000660 DAOPTR010000000
VERSION     DAOPTR010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36078_length_2344_cov_3.768021"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PKI05_11820"
     CDS             <1..712
                     /locus_tag="PKI05_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOA52935.1"
                     /translation="NVLGTVPPLPVIIKAAHSVGALVLVDGAQGVSHRKTDVQALDAD
                     FLVFSGHKMLGPSGVGVLYGKREILESMPPFMGGGSMIREVRMDGFTPGDVPYRFEAG
                     TPPIVPAIGMTAAIDYLEQVGLERIDQYERQLTKRLHEELEKLEFVRILGPTPEYKSG
                     IVSFVVDRIHAHDVAQVLDSVGVAVRAGHHCAMPLHKRLGVTASVRASFYFYNMASEI
                     DRLIEGLDRAVRLFRRRK"
     gene            906..>2344
                     /gene="trpE"
                     /locus_tag="PKI05_11825"
     CDS             906..>2344
                     /gene="trpE"
                     /locus_tag="PKI05_11825"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HOA52936.1"
                     /translation="MSRKRYYPDFESFQQLAEGFDLVPVYRRLVSDSLTPVTAFHKLD
                     AGRCACLAESVTGGEKVGRYSFLATHPFLEIEAWERRVIVNSYTGVSASGAPLLETRQ
                     FISDNPLGELRKRLEGLRVRTLPELPPFCGGAVGYVGYDVVRYYERLPNPPPDDRNIP
                     DLSFGFYDRMVVFDHVTKSLIVVALAHLDRPGWTAEDAYADACRRIDEAVDQLTHSPC
                     ELEPFDICVETEPCVEFTSNFTREDFEEAVRKCIRYIEAGDIFQVVISQRLEVPLQAH
                     PFEVYRALRVINPSPFMFYVRSPSVTLVGSSPEILVRVVNRQVTTRPLAGTRPRGKTE
                     EEDRRLAEELLADPKERAEHVMPVDLGRNDLGRVCRYGSIRLTDVMTVERYSHVMHIT
                     SNVTGELAPGKTAFDALQACLPAGTVSGAPKVRAMEIIDELEPHRRGPYAGAVGYFDF
                     SGNMDTCITLRTIVVQGGKAYVQAGAGIVA"
BASE COUNT          473 a          591 c          713 g          567 t
ORIGIN      
        1 gaatgtcctc gggacagtcc ctccacttcc cgtaattatt aaggcggccc actcggtagg
       61 ggccttggtc cttgttgacg gtgcccaagg cgtgtcccat cgcaaaacgg atgtccaggc
      121 gctcgatgca gattttttgg tcttcagcgg gcacaagatg ctaggaccct ccggggtcgg
      181 tgtgctgtac ggcaaacggg agattctcga atcgatgccc ccatttatgg gtgggggtag
      241 catgattcgg gaagttcgca tggatggctt taccccaggc gacgttccct atcggtttga
      301 ggcgggaacg cctccgattg tgccggccat tgggatgacg gctgcaattg attatctcga
      361 gcaagtgggg ttggagcgaa ttgatcagta tgagcgccaa ctaacaaaga ggcttcatga
      421 ggaattggag aaactggaat tcgtgcgtat tctggggcca acgccggagt acaagtctgg
      481 aatcgtcagc ttcgtcgtgg atcgcatcca tgcccatgat gttgcacagg tccttgacag
      541 tgtgggcgtg gcggtgcgtg ccggccatca ctgtgccatg ccgttgcaca aacgcctggg
      601 tgttacggcc tctgttcgag cgagctttta cttctacaat atggcgagcg agattgaccg
      661 attgatcgaa ggtctggacc gcgccgtgcg gcttttccga cgccgcaagt gacacgtgtt
      721 gtcacaaggc gctgtagcaa catgtcacaa atgatttggt ggccgccagg tcagaacgag
      781 cttctgaggt tctattgtga cgctttcttc ccactgatac gctatgattg tatcccacgc
      841 attgtgagac gatacttaat gagagacgtt gggggcccca tttcaaaaaa tcacagatct
      901 cttttatgtc gcgtaaacga tactatccag attttgaatc gtttcagcaa ctggccgagg
      961 gatttgacct tgtgccggta taccggcgtt tggttagcga ctcgctgact cccgtaacag
     1021 cattccacaa actcgatgcg ggcaggtgtg catgtcttgc agaaagcgta acaggcggcg
     1081 aaaaggttgg ccgttatagt ttcttggcga cgcatccctt tttggaaatc gaggcctggg
     1141 agcggcgagt cattgtgaac tcatacaccg gcgtttcggc gtctggggca ccgctcttgg
     1201 aaacgcgcca gttcatttcg gataacccct tgggtgagct tcggaaacgc ctggaaggct
     1261 tacgtgtccg gacgttgccc gagcttcccc cattttgtgg gggtgccgtt ggctacgtgg
     1321 gatacgatgt cgtccgttac tacgaacggt tacccaatcc gccgccggat gaccgcaata
     1381 tcccggacct gtcgtttggt ttttatgacc ggatggtcgt gttcgaccac gtgacaaagt
     1441 ctctcatcgt ggtggcgttg gcgcatctcg atcgaccagg atggactgcg gaagatgcct
     1501 acgccgatgc ctgtcggcgg attgatgagg cggtggatca actgacgcat agcccttgtg
     1561 aactggagcc ttttgatatt tgcgtagaaa cggaaccctg tgtggagttt acatcaaact
     1621 tcacgcggga agacttcgaa gaggcagtgc gaaagtgcat tcgttatatt gaagcgggcg
     1681 atatcttcca ggtcgtcatt agccagcgtc tggaggtacc ccttcaggcc catccgtttg
     1741 aggtgtatcg ggccctccgt gtcatcaatc cgagtccctt catgttctat gtgcgaagtc
     1801 ccagcgtcac attagtgggg agctcaccgg agattcttgt ccgagttgtc aatcggcagg
     1861 tgaccacgcg gcccttggcg gggacacgac ctcgcggaaa gactgaggaa gaggaccgaa
     1921 ggttggccga agagcttttg gcggacccaa aagaacgggc cgagcacgtg atgcctgtgg
     1981 atttgggccg caacgacctt ggccgggtct gccgctatgg ctcgattcga ctgaccgatg
     2041 tcatgactgt tgagcggtat agtcatgtga tgcacattac ctccaacgtg acgggggagc
     2101 ttgcgcccgg caagacggcc tttgacgccc ttcaggcttg tttgcccgct ggcactgtat
     2161 ccggggcgcc caaggttcgg gccatggaga tcattgacga gttggaaccg catcggcgtg
     2221 gtccttatgc cggcgcggtg ggttacttcg atttcagtgg caatatggat acgtgtatca
     2281 cgttgcggac aatcgtcgtg caagggggaa aggcctatgt gcaagccggg gccggtatcg
     2341 tagc
//
LOCUS       DAOPTR010000661         2344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_36083_length_2344_cov_3.038008, whole genome shotgun sequence.
ACCESSION   DAOPTR010000661 DAOPTR010000000
VERSION     DAOPTR010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36083_length_2344_cov_3.038008"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="PKI05_11830"
     CDS             complement(<1..844)
                     /locus_tag="PKI05_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOA52937.1"
                     /translation="MSIENLNKIFNPYRVAVIGASDTPTSVGYTVLKNLVGSGFRGVV
                     YPVNPKREAVQGIHAYKDVASLPHAPDLAVICTPARTVPGLVHELGEVGTRGIVIISA
                     GFREIGDEGKRLEAQILEEQAKYPGMRIIGPNCLGIIVPGISLNASFAAATPPKGHIG
                     FISQSGALCTSVLDWAIDQGIGFSYFVSVGNMLDVTIADLIDYFGSQTETQSIILYIE
                     SITEAREFMSAARAFARTKPIVAYKAGRFAESAAAAASHTGAMAGVDAVYEAAFQRAG
                     IERIF"
     gene            1357..2001
                     /locus_tag="PKI05_11835"
     CDS             1357..2001
                     /locus_tag="PKI05_11835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOA52938.1"
                     /translation="MESSLQEPALTDEQLVEKARNGDFEAFETLVNRYERRVFLLALR
                     IVRRSHDAEEVVQKTFLSVLEHLSDFRGDSAFATWLLKIATNHALGLLRREALRTTLP
                     LAGGSHDVEDDDGLPKPRYIAEWRDSPDEIVSRKETRDLVEQALQELDEKYRLVFVLR
                     DVEGFSTAETAQILHLTEANVKVRLLRARLMLREKLTELFGDPERVLPPHSHEE"
     gene            complement(2145..>2344)
                     /locus_tag="PKI05_11840"
     CDS             complement(2145..>2344)
                     /locus_tag="PKI05_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52939.1"
                     /translation="IYLVRVIEYQPPIEQLREMFFRQTDPVYLAAGQIEFNEIAMRWI
                     RGLEDDAGLKWNRPARRGARD"
BASE COUNT          562 a          670 c          606 g          506 t
ORIGIN      
        1 ggaagattcg ctcgatcccc gcccgctgga aggccgcctc atagacggca tccacacctg
       61 ccatggcccc ggtatgggaa gcagccgcgg cagcggattc cgcaaaccgc ccggccttgt
      121 aggcaacgat cggcttggtt cgggcaaagg cgcgggccgc ggacatgaat tcccgagcct
      181 ctgtgatgga ttcaatgtaa agaatgatgg actgcgtctc cgtctgcgat ccgaaatagt
      241 cgatcaaatc ggcgatggtg acatcgagca tgttgcccac ggagacgaaa taggagaatc
      301 caatcccctg gtcaatggcc caatcgagga cagacgtgca caacgcacca gactgcgaga
      361 taaacccaat atgtcccttc gggggcgtgg ctgcggcaaa gctggcgttc aaactgatgc
      421 caggaacgat gatccccaga cagttcggac caatgatccg catgccggga tacttggcct
      481 gttcctccaa aatttgagcc tcaagccgct tcccctcatc cccaatttcc cggaaaccgg
      541 cggaaatgat gacaatccca cgcgttccca cttcgcccaa ctcatggacc aaaccgggca
      601 ccgttcgtgc cggcgtacaa atcaccgcca aatcgggggc atgcggcagg cttgcgacat
      661 ctttatatgc atggatacct tggacggcct ctcgctttgg attgacagga tagacaactc
      721 cccgaaatcc ggagccgaca agattcttga gaacggtata accgacactt gtcggagtgt
      781 cgctcgcccc aatcacggcg acacgatacg ggttaaaaat cttgttcagg ttttcaatac
      841 tcacggcagg ctcctgttgc gttaagctcg ttttatggca aacaaaaccc catcatgaat
      901 cggcctcagg caagccgaga ttgttctgcg aagcgaacgg ctcaactttt aaaatagccc
      961 atctgtgtca tcgtcgaaag tggcgcaaag attgacgggc gggaaaaatg ccgcgctttg
     1021 ccaagcatga aacgattgtg cgggatgaaa cggctaggcg accttaagcg aacgcaagga
     1081 acaatatggc ttactaaaac tgtcacaaat cacaaaaacc tacttgtcag ctctactttt
     1141 gttacggggg atttcatccg aaagtttcca cttcctacag attccatcat gccgcggttg
     1201 tcgaaagccg caaaacctgg ataatgaagg acctcgccag tggttccata gcccgtggtc
     1261 tgaaagatct ccgaaaatcc agattgtacc gcaggggact aagggacagg tcctttgtaa
     1321 ccgtgggatg acagcggcaa ttgtgataaa aatttcatgg aatcgtcact acaagaacca
     1381 gcactgaccg acgaacagct tgtcgaaaag gcgcgaaacg gtgatttcga ggcatttgag
     1441 accctcgtca atcgctacga acgccgtgtg ttcttgctcg ccctacgaat cgtgcgtcgg
     1501 tcccacgatg cggaagaggt tgttcagaaa accttcctaa gtgttcttga acacctttcg
     1561 gactttcgcg gtgactcggc atttgccacc tggctcttga agatcgccac caaccacgcg
     1621 ctcgggctgc ttcgacggga ggccctccgt acgaccctgc ctcttgctgg tggttcccac
     1681 gacgttgagg acgacgatgg attacccaag ccccgctaca ttgcggaatg gcgtgactct
     1741 ccggatgaga ttgtctctcg caaggagacg cgcgatttgg tcgagcaggc cctccaggag
     1801 ttggatgaaa aataccgtct ggtgtttgtg ctgcgtgatg tcgaggggtt ttccacagcc
     1861 gaaactgccc aaatccttca tttgaccgag gcgaacgtca aagtccgact tcttcgggca
     1921 cgcttgatgt tgcgggaaaa gctcacggag ctgtttggtg acccagagcg cgtccttcct
     1981 cctcactccc acgaggagta aacttcgtct tttccatcgc caggcaaaag ccttggctca
     2041 gatacagccg tactgcccat gcagtcaccc cgccttggcg accttcatcg gaaacctcgg
     2101 ctctgccgtg tcgtcacatt gaaacggcaa cagtcttatc cgagttaatc ccgggcaccg
     2161 cgccgggcag gccgattcca tttcaatccg gcgtcgtctt ctagcccgcg aatccatcgc
     2221 atcgcgatct cgttgaactc gatctgccct gccgccaagt aaactgggtc agtttgccgg
     2281 aagaacattt cccgcaattg ctcaatcgga ggttgatact caattacccg gaccaaatag
     2341 atga
//
LOCUS       DAOPTR010000662         2343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_36106_length_2343_cov_3.045892, whole genome shotgun sequence.
ACCESSION   DAOPTR010000662 DAOPTR010000000
VERSION     DAOPTR010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36106_length_2343_cov_3.045892"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="PKI05_11845"
     CDS             complement(<1..1004)
                     /locus_tag="PKI05_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52940.1"
                     /translation="MRSIFAGGLSLITVILVSLVGNAGLASDVSELAGLVPKDAVIAL
                     VVREPGKLVEEWEKSPIRQALRANPAFQQALKNPGMQQAETLIGLIELQTKSNRYDLL
                     KKMTAGGIVLAVLPKNKVILVIEAEDGEVLRQVHEFILGLAKLDAANKNHPDRVRSAD
                     YRGVTGWTFGGDEFHAIIGNRLILSNKAEVIKDVVDLALDQPPETLKSNPRFMAAMQV
                     VKQKNVSLFLDLDVLRQLPELKSGLAQSKEPIPVLLLGGAVAALEKAPWMALGIDIEQ
                     NRLSGTLRLGEISEWPSALSFAVAPPDDGILPPLEVPNLLASMSWYRNLRDFYAQKNE
                     L"
     gene            complement(1135..2043)
                     /locus_tag="PKI05_11850"
     CDS             complement(1135..2043)
                     /locus_tag="PKI05_11850"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOA52941.1"
                     /translation="MASSDSKMHHDRPIPVLALTWLELRTLFRGWLVRIWIVAALLLS
                     FVTTLSGWRALPLPVMIASLAFPFLVFPWFVVIVIPGTNAVVATRMESIADGILSRPI
                     TRLEFLVAAWLARAVTVLAVLALAVVPAALVAGLADRPVESDPVASYGVIMASLVVIL
                     VVLGVLSLGFFVGIILRNAWLALLVVLFIWYPINAVFTTFRLEELSPISLNQALPTLM
                     RKTWQTAEEVTEIRPRDLIVLQQQADQFLQSLTGRPRRPAKEPAFFEHDLEYQDIKPW
                     RVVLSYAVPIVVFLGLSYVCFYFRDL"
     gene            complement(1976..>2343)
                     /locus_tag="PKI05_11855"
     CDS             complement(1976..>2343)
                     /locus_tag="PKI05_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52942.1"
                     /translation="SMAEMKRRLRGDSFFLDLGTTEEVRQRLDAMLQNLNGITYQWLD
                     THRLRVAVSADVRRAATLAEVLRCVDQAGAILHSVQSGLGATEDAYLQLLQEDELHGF
                     KRFQDAPRPTHPSSSPNLA"
BASE COUNT          544 a          687 c          664 g          448 t
ORIGIN      
        1 agttcatttt tctgggcgta gaaatcgcgg agattgcggt accagctcat gctggccagg
       61 aggttgggca cctcaagcgg cggcaagatt ccgtcatcgg gtggggccac cgcgaaggag
      121 agcgcgctgg gccactcgct aatctcaccc agccggaggg tcccactgag ccggttctgt
      181 tcgatatcga tgccgagtgc catccagggg gccttttcca aggccgccac ggcccctcca
      241 aggagaagga caggaatcgg ttccttgctt tgggcgaggc ccgatttcag ctccggcaac
      301 tgtcggagca catccaaatc gagaaacaga ctaacgtttt tctgtttaac gacctgcatg
      361 gccgccatga atcttgggtt actcttaagc gtctccggcg gctgatcgag tgccagatcc
      421 accacgtcct tgatcacctc cgccttattg gacaggatga gtcggtttcc tatgatggca
      481 tgaaactcat caccgccaaa agtccagccc gtgaccccgc gatagtcggc cgatcggacg
      541 cgatcggggt gattcttgtt tgccgcatcg agcttggcca gccccaggat gaactcatga
      601 acctggcgga ggacctcgcc atcctcggcc tcaatgacga ggatgacctt gtttttgggc
      661 aagacggcca gaacaatccc gcccgccgtc atctttttga gaaggtcata acgattggac
      721 ttggtctgca attcgatgag gccgatgagg gtctcagcct gttgcatgcc gggattcttg
      781 agggcctgtt gaaaggcagg gtttgcccgc agggcttgcc gaatgggcga tttttcccat
      841 tcttccacca actttcctgg ttcccgcacc accaaggcaa tgacggcatc cttgggcacc
      901 aggccggcca attcgcttac atctgaggcc aagccggcat ttcccacgag actgacaagg
      961 atcacagtga taagactcaa gccgccggca aatatggaac gcatgatggg gtacctccaa
     1021 caagtgttcg gacagttctt cccgccacgg ccggaccttc tttctcgtaa ggcctggccc
     1081 aaaaggtgat tcatagtgta atacagcggg cggcggaaaa atgtcgcacc gattttacag
     1141 atcacggaaa tagaagcaca cataagacag gccgaggaac acgacaatcg gcacagcgta
     1201 actcaaaacc acccgccagg gtttgatgtc ctgatattcc aggtcatgct cgaagaaggc
     1261 tggttccttc gcggggcgcc gcggtcgccc ggtgaggctt tgcaagaact gatcggcctg
     1321 ctgctggagg acgatcaagt ctctcggtcg gatttcggtg acttcttccg cggtttgcca
     1381 ggtctttcgc atcaatgtcg gcagggcctg attgaggctg atcggggaaa gctcctccaa
     1441 gcggaaggtt gtgaacaccg cattgatcgg ataccagata aacagcacca ccagcagcgc
     1501 cagccaggcg ttgcgaagga taatccccac aaaaaaccca aggcttagca cccccaacac
     1561 aacaaggatc actactaggc tggccatgat gacgccgtaa ctggccacgg gatcgctttc
     1621 cacgggacga tcggccaatc cggcgacgag ggcggcaggc accacagcca aggccaatac
     1681 ggcaaggacg gttacggccc gggcgagcca ggccgccacg aggaactcca gccgggtaat
     1741 tggccgactc aaaatgccat cggcaatact ttccatccgc gtagcaacca cggcatttgt
     1801 cccagggatc acaatgacca caaaccacgg gaacaccaaa aatggaaacg ccagtgaggc
     1861 gatcatcaca ggaagaggaa gcgcccgcca cccgctcagc gttgtgacga agctgagcaa
     1921 gagggcggcc acgatccata tccgaaccaa ccagccccga aacagcgttc gcaattcaag
     1981 ccaggttagg gctagaactg ggatgggtcg gtcgtggtgc atcttggaat cgcttgaagc
     2041 catgcagctc gtcctcttgc aaaagttgca aataagcgtc ctcggtagcg cccaggccag
     2101 actgaaccga atgcaggatg gctccggcct gatccacgca tcgcaagact tcggccagag
     2161 tggctgcccg acgaacgtcc gccgaaacgg ccacgcggag gcggtgagta tccaaccact
     2221 ggtaggtgat gccattgaga ttctgaagca ttgcgtccaa gcgttggcgg acctcttccg
     2281 tggtccccag gtcgaggaaa aagctatcgc cgcgtagccg ccgcttcatc tcggccatcg
     2341 agc
//
LOCUS       DAOPTR010000663         2341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_36154_length_2341_cov_2.798775, whole genome shotgun sequence.
ACCESSION   DAOPTR010000663 DAOPTR010000000
VERSION     DAOPTR010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36154_length_2341_cov_2.798775"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..>1158)
                     /locus_tag="PKI05_11860"
     CDS             complement(184..>1158)
                     /locus_tag="PKI05_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52943.1"
                     /translation="GIISTVRATRTASREDLVRDATFYAVREQLDTLLFNHFEGLARK
                     NPSRWQSLIAWHRYLLAGAALEEPRLRDLLRDTYRFVTSRGEMTFPEIVAASRTSTVY
                     ATDDDFVIWYNPNRHQESWINEVFADFPAPCVHTLRSFEETLLVLMVSDLQNEQISVD
                     LRVAAPSSEDFAELVLGIQELSNVPHEWEQFLADLPALIRIGDYHGDIPVLAFLSEQA
                     ELQRTFEGIKKQGTMPCGFQRLVEAHFQYDKPKPNEVILNRRHPLIRRALEQSTRVPL
                     ASVVRLLVIEALRMAGLRPDAKIRQVEQEDLAWIAEALWGRDEGRRRK"
     assembly_gap    1159..1168
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1169..2138)
                     /locus_tag="PKI05_11865"
     CDS             complement(<1169..2138)
                     /locus_tag="PKI05_11865"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA52944.1"
                     /translation="MSNESDSPQIANRPKSSWQKRVPFQVDVAGVIRLMGQSLYSRSD
                     AAIRELLQNAHDGIMRRRAIDLQYRGRIDIRQHPADKVLEVSDDGIGLTPEEVEKYLG
                     TLGVGISGLLKRDLPNRGGQSLNLIGEFGIGLFSAFLLADRVEVITRHRQDGEAVRWE
                     AGPDSDICISSCDRSEPGTTVRLHLKPDQYPYATDEALLERAVKEYADFLPIPIHIND
                     RPQRVNIGSACWLEFTPDTHALELELESFFGETPLDLVVLRTEKPVTIRGALYVTPQR
                     TPGFTDAPTVTVTVQRMIISRRVYGLLPDWASFVRGVLELPDCSPTA"
BASE COUNT          554 a          651 c          600 g          526 t
ORIGIN      
        1 gtgccgactc cttatcaccc aagttatccc ccaagaaaac ctcgaggttc gccgaaagaa
       61 ccaattgacg tcgttctcgc tccgtaacag cgattcttta ccgtgcgttc aatttatcga
      121 gattccgatc caactgaaac gattcggacg aaaagcaggt catgtgccag aaatctcatc
      181 ggtttacttt ctccgccgac cctcatcgcg accccaaagc gcctctgcga tccacgctaa
      241 atcttcctgc tcgacctggc ggatctttgc gtccggccgc agccctgcca ttcgaagggc
      301 ctcaatgaca agtagccgca cgacacttgc caggggcacc cgcgttgatt gttcaagcgc
      361 tcgcctgatt agcggatgac ggcggttcaa aatgacctcg ttgggcttcg gtttgtcgta
      421 ctggaaatgg gcctccacga ggcgctgaaa gccacacggc atggttccct gttttttgat
      481 cccctcaaat gttcgctgaa gctcggcttg ttcactcaaa aacgccagca cgggaatgtc
      541 tccatgatag tccccaattc gaatcaatgc tggcaaatcc gcgagaaact gttcccactc
      601 atgaggaacg ttcgatagct cctggattcc caggaccagt tcggcgaaat cttcgcttga
      661 tggggccgcc acacgcaggt caaccgagat ttgttcattc tgtaggtccg agaccatgag
      721 caccaaaagt gtttcctcaa agcttctcag ggtatggaca caaggcgccg gaaaatcggc
      781 aaagacctcg ttgatccaag attcctggtg acgattggga ttgtaccaaa tgacgaaatc
      841 atcatccgtt gcatagaccg tcgaagttcg gcttgccgcg acaatctcgg gaaaggtcat
      901 ctcgccccga gacgtcacaa aacgataggt gtcccgcaaa aggtcgcgaa ggcgaggctc
      961 ctccagagcg gcccccgcaa gtaaataacg atgccaggcg atcagcgatt gccaacgact
     1021 tgggtttttt cgggctaacc cctcaaagtg attaaatagg agcgtatcca actgctcccg
     1081 tacggcgtaa aatgtggcat cgcgaaccag atcctcccgg ctggccgtgc gcgtcgcccg
     1141 gacggtcgag atgatgccnn nnnnnnnnta gctgtaggtg aacagtcggg aagttcaagg
     1201 acaccgcgaa caaagctcgc ccaatctgga agcaaaccat agacccttcg ggagataatc
     1261 atccgctgca ctgtgaccgt aaccgtgggg gcatccgtga aacctggcgt tcgttggggc
     1321 gtcacgtaaa gagcgccgcg gatggtaacc ggcttctctg tccgtaaaac gaccaaatcc
     1381 aaaggcgttt ccccgaagaa ggattccagt tccaattcca aggcgtgggt gtccggtgtg
     1441 aactctagcc aacatgcgga tccaatgttg accctttgcg gtcgatcgtt aatgtgaatc
     1501 gggataggca agaaatccgc atattccttg accgcccgtt cgaggagcgc ctcgtccgtg
     1561 gcatacgggt attgatcggg cttgagatgt aaccgaaccg ttgttcccgg ctctgaacgg
     1621 tcacaggaac tgatgcagat atccgaatcc gggccagcct cccaccgaac cgcctcacca
     1681 tcctgacggt ggcgggtgat cacttccacg cggtcggcta ggagaaatgc gctaaaaaga
     1741 ccgatcccga attccccaat cagattgagt gattgaccac cacgatttgg caaatctcgc
     1801 ttcagaagcc cactgattcc cacccccagc gttcctaggt atttttccac ttcttcaggt
     1861 gtcagtccaa tcccgtcatc actaacctcg agtaccttat cagctgggtg ttgacgaata
     1921 tcaatacggc ctcggtattg taaatcgatc gcccgccgcc gcatgatgcc atcatgagca
     1981 ttttgaagga gctcacgaat tgctgcatcc gaccggctat agagggattg ccccatgagc
     2041 cggatgactc cagcgacatc gacttgaaaa gggactcgct tttgccacga actcttggga
     2101 cgattggcta tttgagggga atctgattcg ttcgacattg agacaactta attgcgccat
     2161 ttctcggcaa tggcaaagat ggaagcaggc tggttaatgt gaattagtga ccttacgtgg
     2221 caagacgtcg gctactttcg tgattatgac ggcttagggc actttggtca atgaaccgtg
     2281 ggccgcttaa cccagttgaa agagcgtggc tgcattggcg ctcgttactt gtgcgagatc
     2341 a
//
LOCUS       DAOPTR010000664         2340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_36162_length_2340_cov_4.409190, whole genome shotgun sequence.
ACCESSION   DAOPTR010000664 DAOPTR010000000
VERSION     DAOPTR010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36162_length_2340_cov_4.409190"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="PKI05_11870"
     CDS             <1..865
                     /locus_tag="PKI05_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52945.1"
                     /translation="WDERLERAIGEKRWADAEAVLTAAESSGLTAWTEKAWTRLSEVR
                     KESVLGKALFLGRQKRLKEALELCEKTSETLPITAVASVAMSVLRLNRSGIQDRDLAM
                     VEGWLLKARESNPNDKRIAIDLASFLNLAGRYEECEKAYRELLARKDLSDMEKALIKN
                     NLAYILAAKGRSVSGAASEGRLQEAESLIEQAIAVIGPIGSLLDTRTVVRLGLGKNRE
                     ALSDAQQAVVEEPTPLSYFHLVEALIAVGDTPTALREWQRACKDFGLTPEALPPVERE
                     RFRIVAQRLEG"
     gene            complement(1052..1126)
                     /locus_tag="PKI05_11875"
     tRNA            complement(1052..1126)
                     /locus_tag="PKI05_11875"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1089..1091),aa:Pro,seq:tgg)
     gene            complement(1302..>2340)
                     /locus_tag="PKI05_11880"
     CDS             complement(1302..>2340)
                     /locus_tag="PKI05_11880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOA52946.1"
                     /translation="NFAWRSWIVSKEKRLGRGLEALLGKLDSATQAPTGGTSAGAVSF
                     DVPPVGLGTIGTANSLSDSVPAADTQPTGVIEIPIDEIDPNPFQPRQEINSDEIVQLA
                     DSLNNHGLLQPIVVRRHRGRYQLIAGERRFRAAKLAGWDKVPSRVVEADDRQLAELAI
                     VENLQRKDLNPLEKARCFQRYLEEYGVTQEELASRLSLDRSTISNFIRLLDLPEPVQQ
                     ALREGRITQGHARALLSLDSEEDQIALCQQIEQEGLSVRRVEQIVQERQRATAGLTVV
                     HPESSSRPGRSSEHLAALEQEFRAALGLRVSLTHNGRGKGKLVVYFQSHEEFDLLRDR
                     ICGQEKHRAAG"
BASE COUNT          520 a          654 c          692 g          474 t
ORIGIN      
        1 gtgggatgaa agactcgaac gtgcgatcgg cgaaaagcgt tgggccgatg cggaagccgt
       61 tctcactgcg gcagaatcga gcggcttgac cgcttggacc gagaaggcgt ggacgcgatt
      121 gagcgaggtc cgaaaagaat ccgttctcgg aaaagccctc tttttggggc gtcagaaacg
      181 attgaaagaa gccctagagc tttgtgagaa aacgtcggag acgctgccaa taacggcggt
      241 ggcctcggtc gccatgagtg tgctgcggtt gaaccgatca ggcattcaag atcgtgacct
      301 agcgatggtc gaaggctggt tactcaaggc cagggaatcg aacccgaatg acaagcgaat
      361 tgccatcgac ttggccagtt ttttgaacct cgccgggcgg tatgaagaat gcgagaaagc
      421 ttaccgagaa cttttggccc ggaaggacct ttcggacatg gaaaaagccc ttatcaaaaa
      481 caatctcgca tacattttgg ccgccaaggg gcgcagtgtg tccggggcag caagcgaagg
      541 tcgccttcaa gaggccgaga gtctaatcga gcaggccatc gctgtcatcg gcccgatcgg
      601 gagcctgctc gatacacgga cggtggtccg cctaggcctt ggtaaaaatc gagaagccct
      661 ttccgatgcc caacaggccg tggtcgagga gccaacaccg ttaagctact tccacctggt
      721 agaggcgctc atcgcggtgg gcgatacacc tacggccctg agggaatggc agcgggcgtg
      781 caaagatttt ggcctgacgc cggaggcact ccccccggtt gagcgggaaa ggttccgtat
      841 cgtcgctcaa cgcttagaag gatgaaccta tcaaaggggc tcgggtaggt gcagaaaatg
      901 gggcaagttg cgcaaaagtt ttggcaaaag cctaccccaa aaattgatgg aacgagacgc
      961 tccgaactgc ggaaaacccg aatgaggaca aacaccaagc gctgcccaat tgacctcctc
     1021 agcgggccga tcgaatgagc tcaaaagtaa gtcggggcgg ccggattcga accggcgacc
     1081 ctctgctccc aaagcagatg cgctagccag gctgcgctac gccccgcatc ttgccaaaaa
     1141 ggcctcatgc atccgtatct taccatccca ccggccgatt gttaaggccg ccctgccact
     1201 cccgtgacct tgctgggtga ctcgtcgcat ttgcgcggag gacggcactt gaaagcgcgg
     1261 ccccgctgga gtggggcttt ctccggcggc gggacatcgg ttcagccggc tgcccggtgt
     1321 ttctcttgtc cgcaaattcg gtctcgcaaa agatcgaact cttcatgtga ttggaaatag
     1381 acgaccaact tgccttttcc gcgtccgtta tgggtaagtg agacccgcaa tcccaaggcc
     1441 gcacggaact cttgctccaa agcggccagg tgttcggacg atcgccctgg gcgggatgat
     1501 gactcggggt ggacgacggt caggcctgcc gttgctcgct gcctttcttg gacgatttgc
     1561 tcgactcgcc ggacgctgag gccttcctgt tcgatttgct ggcaaagggc gatttggtcc
     1621 tcctcagaat cgagcgagag caacgcgcga gcatgtccct gggtgattcg tccctctcgc
     1681 aaggcctgtt ggacaggctc cggtagatcg agaaggcgga tgaaattgga gatggtcgaa
     1741 cgatccagac tcagtcggct ggccaactct tcctgggtta cgccgtattc ttccaggtat
     1801 cgctggaaac aacgggcctt ttccaacggg ttgaggtctt ttcgctgcaa gttctccaca
     1861 atggcaagct cggcaagctg tcgatcgtcg gcctcgacaa cccgcgacgg gaccttgtcc
     1921 caaccggcaa gtttcgccgc gcggaagcgg cgttccccgg caattaactg atagcggccc
     1981 cgatgccgcc gcacgacgat cggctgaagt agcccgtggt tattgaggct atcagccagt
     2041 tgaacgattt catcggaatt gatttcctgc cggggctgaa acggattggg atcgatctca
     2101 tcaatgggga tttcgatgac gccagtcggc tgggtatctg ccgcgggaac actgtcagac
     2161 agggaatttg ctgtacctat tgtaccgaga cccacaggcg ggacgtcgaa actcacggcc
     2221 cctgcggacg tacctccggt tggggcctga gtagcgctat ccaacttacc caataaggct
     2281 tccaaaccac gaccaagtct tttttcctta ctcactatcc aagacctcca tgcaaagttc
//
LOCUS       DAOPTR010000665         2340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_36165_length_2340_cov_3.592998, whole genome shotgun sequence.
ACCESSION   DAOPTR010000665 DAOPTR010000000
VERSION     DAOPTR010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36165_length_2340_cov_3.592998"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            569..1375
                     /locus_tag="PKI05_11885"
     CDS             569..1375
                     /locus_tag="PKI05_11885"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOA52947.1"
                     /translation="MPVAQGTWTTVFRATVASNPPNRPPRYAIKTLRTDLTDTAIARG
                     HLSREAEVGRTVVHPHVVPILAWRLTGDWPYVVMPWLEGTSLQQLLSQRGAIPTADAI
                     WFVRQIAEGLAALHGAGWLHADLKPSNIMVNPEGHVTIIDFGFAQRWEEGSLSPREVV
                     GTLDYMAPECIFRHAPWGPKSDLYSLGVIFFEVLTGQRPYCVRSLREAARVHRQRRVL
                     DVRILNPSVSHEVSRLIRQMMSPIPERRPASAAAVAEHLVRLEIEHFTFR"
     gene            complement(1535..>2340)
                     /locus_tag="PKI05_11890"
     CDS             complement(1535..>2340)
                     /locus_tag="PKI05_11890"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOA52948.1"
                     /translation="VKDVSNVERTPAVANPTSMPQAETHSNDEQCVSAGLAEEPTSMA
                     EPQVAGSIESEANMEGRSGGGDKTCLSSDHDECVVSAAVTDEHELTDNCSRQVEVVAS
                     ESSESEVSDADDDEAIIGLRPAVVENERLWNTFACELQHRWQALSELRRQISAKGETF
                     EPAKDFDIVVDVARILHTIKSAAMVVPIEIVTRTTHALESLIEAGSDRNEWPAQAFNR
                     YCDWLEMFLNPVDQTWDSWRETGNYLAADLEQQLAQPAEAPQTTTSCQG"
BASE COUNT          479 a          661 c          622 g          578 t
ORIGIN      
        1 ataaacatcc agagtgatat cgcgaagagc ttcgacaacc acgtcgccca tgacaaaaag
       61 tttgctcaca ttgcgcatct ggacaaacgg ggctgtagtg tcgtcagtat gcatactttt
      121 ggactgatcg tcgcattttg tcgattcgcg caaggccata tctttcaaag cgctatggca
      181 aatgtttgct gtcaaagttc accgcctgaa aaaggcgttt ttccatgtcc attattgcct
      241 gcgaccaaac ggattgcgcg ggattttcgt aaggactttt ggcctttatc gagtttctgg
      301 ccttggtcgg ttactggcgg ttgctcggtg tgcacgcatt atgactgttg gaatgattgt
      361 acccgccatc tcacgtggct ctgcccgcgt ggagcctctt accgctgttg gccgatgaac
      421 gagcaagaga gtttgtcagc cggggtttga ctcgcaacgc aatgtgtcta tgttaactta
      481 cgccatccaa gcccgatcgc acttcgaaaa catagggagg gcagaccaag gttttgtccc
      541 gcgtaccctg ggggagtggc ttctcctcat gcccgtcgcg cagggtacat ggacaaccgt
      601 gtttcgggca actgtggcgt cgaatccacc caatcggccc cctcggtatg ccatcaagac
      661 attgaggaca gatctgactg acactgccat cgcccgaggt cacctttccc gtgaggcgga
      721 agtcgggcgg acggtggtcc atccccacgt tgtgcccatc ctcgcttgga gattgactgg
      781 cgattggccg tatgtcgtta tgccctggct agaagggacg tccttacaac aattgcttag
      841 tcaacgcggg gcgattccta ctgcagacgc catttggttt gttcgacaga tagccgaagg
      901 cctggcggca ctgcacgggg caggttggct tcacgctgat cttaagccgt ccaacatcat
      961 ggtcaatccc gagggtcatg tcaccatcat cgattttggt tttgcccaac ggtgggaaga
     1021 agggagtttg tctccgcggg aagtcgtcgg cacgttggac tacatggccc cggaatgcat
     1081 ctttcgacat gccccctggg gacccaaaag cgacctttat agcctgggtg tgattttctt
     1141 tgaagtcctg acaggccaac gcccatattg cgttcgcagc cttcgagaag ccgcccgggt
     1201 tcatcggcaa cggcgagttc tggatgtccg catcctgaat ccttctgtct cacacgaagt
     1261 gagtcgccta attcgccaga tgatgtcacc cataccggaa cggcgtcctg cttccgcagc
     1321 agccgtggca gaacacctgg ttcgcctgga aatcgaacac ttcacatttc gttaacaagg
     1381 tgttgccgtg ttcggcggta ctgaaaggga ctcctgcgga ttgaccgctc cttttggcgt
     1441 gtgtcgtaaa tcgacctcag ccagatgaac agaaccaacg gcggcgtccg gtgcacggtc
     1501 cgccttgtct cgtgccacgt gcgatagcca cagtttatcc ttggcaagat gtcgtggtct
     1561 ggggtgcttc cgcaggttgg gccaactgtt gttcaagatc ggctgccagg tagtttccag
     1621 tctctctcca agaatcccac gtctgatcaa ccggattgag gaacatttcc agccaatcgc
     1681 aataacgatt aaacgcctgg gcaggccact cgttgcgatc ggaaccggct tcaatcaggc
     1741 tttcaagggc atgagtcgtc cgagtaacaa tctcgattgg aacaaccatc gccgcggact
     1801 taatggtgtg gagtatccgc gccacatcaa caacaatgtc gaagtccttg gcgggctcga
     1861 acgtctctcc tttcgcagaa atttgccttc tcagctccga tagtgcttgc cagcgatgtt
     1921 gaagctcgca agcaaatgta ttccacagtc gttcgttctc gaccacggct ggccgcaaac
     1981 caatgatggc ctcgtcgtca tccgcgtcgg aaacctccga ttcagaggac tcgctcgcaa
     2041 caacctcgac ttgcctcgaa caattgtccg tcaactcatg ctcgtccgta accgctgcgc
     2101 taacgacaca ttcgtcatga tcactgctta ggcaggtttt gtcgcctccg ccggatcgcc
     2161 cttccatatt tgcttccgat tcgatggacc cagcaacttg gggttcggcc atgcttgttg
     2221 gctcctcggc cagtccggct gacacgcact gctcatcgtt cgagtgcgtc tcggcttgtg
     2281 gcattgaagt aggattcgcc acagcaggag tgcgttccac attgctgacg tcttttacgt
//
LOCUS       DAOPTR010000666         2338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_36212_length_2338_cov_3.287341, whole genome shotgun sequence.
ACCESSION   DAOPTR010000666 DAOPTR010000000
VERSION     DAOPTR010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36212_length_2338_cov_3.287341"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PKI05_11895"
     CDS             <1..687
                     /locus_tag="PKI05_11895"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOA52949.1"
                     /translation="GMSTAATMIVNVKGEDRRFISTPGANVVFKAEDIPTAWTHAARV
                     FYVGGYLMMPGLETDDFVYLLRDLRAKGVITVVDVVLMERRDFWPILRKVLPEVDYFL
                     PNDDEGEIITGVADPLEQAQRFHEAGAKNVVITLGEKGCLFYGEGAKFTAGVYPTTFV
                     SGAGAGDAFDAGFIAGLLWGGSPEDCVRWGSALGASCVRAIGTTEGVFTREEAVAFMA
                     SHELPISKLK"
     gene            747..1736
                     /locus_tag="PKI05_11900"
     CDS             747..1736
                     /locus_tag="PKI05_11900"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOA52950.1"
                     /translation="MRIGVLTSGGDAPGMNACIRAVVRAAISMGHEVVGIYRGYAGLL
                     REEFYKDERGHPMMNLRSVSDISRRGGTILHTSRCKEFYTDDGLRKAAEVLRRNKIDA
                     LIPIGGDGTFRGAVALAKYWDGQIVGCPGTIDNDLIGSDYTIGFATAVHTAVEAVDKL
                     RDTADAHERMFLVEVMGRHSGYIALYTAIAAAAEIACIPETPTDTGEIVRLLHDLKLR
                     GKTSVMIIVAEGDERGGAAVLQKALCEVKCPYEVRVLALGHLQRGGSPVPQDRILASR
                     LGDFAVHAILQGKSGVMAGEQKGELVLTPLEETFAAHRPVPEVYVKLLETLAT"
     gene            complement(2026..>2338)
                     /locus_tag="PKI05_11905"
     CDS             complement(2026..>2338)
                     /locus_tag="PKI05_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52951.1"
                     /translation="TASQNPLLDAQAALLKDNKTLAVAIVNPTGEKQLCTLRVAGATV
                     GGSGKVWDVAGNDPMDYNDPGKPPRIELRSRGEVSHADSFEVAPYSATIVQFPIVRAG
                     N"
BASE COUNT          486 a          583 c          720 g          549 t
ORIGIN      
        1 ggcatgagca cggcggcaac gatgattgtt aacgtgaaag gcgaagatcg ccgattcatc
       61 agtacgccag gggccaatgt ggtctttaag gccgaagata ttcccacggc ctggactcat
      121 gctgcccgtg ttttttacgt cggtggatat ctaatgatgc cggggctgga gaccgatgat
      181 ttcgtgtacc ttctgcgcga cctgcgggca aaaggcgtga ttacggtggt cgatgttgtt
      241 ttgatggagc gacgggattt ttggccaatc ttgagaaaag tcctccccga ggtcgattat
      301 tttttgccta acgacgacga gggggaaatt attacgggtg tagctgatcc gttagagcag
      361 gcccagcgtt ttcacgaagc tggcgcgaag aatgttgtca tcaccttggg agaaaaagga
      421 tgtcttttct atggagaggg ggcgaagttc acggcgggtg tttatcccac cacgtttgtc
      481 agtggtgcgg gggccggaga tgcgtttgat gcgggattca ttgcgggatt actgtggggc
      541 gggtctcccg aggattgtgt ccggtggggc agcgctctgg gggcaagctg tgtccgggcg
      601 attggaacaa cagaaggtgt tttcacgcgg gaagaggccg tggcttttat ggcttcacac
      661 gaacttccta tctcaaaact gaaatgaatt gaggtggatt tcgtgcaacc gaaggtactt
      721 cccggaaaga gataggagtg agtgatatgc ggatcggcgt tttgacgagt ggtggtgatg
      781 cgccggggat gaatgcctgt attagggccg tcgttcgggc tgccatttcg atgggccacg
      841 aagttgtcgg tatttaccgc ggttatgcag ggttactccg cgaagagttc tacaaggatg
      901 agcgcggtca tccaatgatg aatttgcgga gtgtttctga tatttcacgg cgtgggggaa
      961 cgatcctcca tactagccgt tgtaaggagt tctacacgga tgacggatta cgcaaagcag
     1021 cagaggtcct ccggcgtaac aaaattgacg cgttgatccc cattggtggt gatggcacgt
     1081 tccgcggggc ggtggctctg gcaaagtatt gggacggaca aattgtcggc tgccctggca
     1141 caattgataa cgatctgatt ggttcggact atacgattgg ctttgcgacc gccgttcaca
     1201 cggccgttga ggccgttgac aaacttcgcg acacggcaga cgcccatgaa agaatgttcc
     1261 tggtcgaagt catggggcgg catagtggct acatcgccct ttatacggcg atcgccgcag
     1321 cagccgagat tgcttgtatt cccgaaacac cgacggacac cggggagatt gtccggctcc
     1381 tccacgattt gaagttgcgg gggaaaacat ccgtcatgat cattgtggcc gagggtgatg
     1441 agcgcggagg ggcagccgtc cttcaaaaag ctctctgcga ggtgaaatgc ccctatgagg
     1501 tcagagtcct tgccctggga catctacagc gcggtgggag ccccgtccct caagaccgta
     1561 tcctcgcctc gcgacttgga gatttcgccg ttcacgcgat tctccaagga aagtcgggcg
     1621 tgatggctgg ggaacaaaaa ggtgagcttg tcttgactcc gctcgaggaa actttcgccg
     1681 cgcatcggcc ggtgcccgaa gtgtacgtca aacttctgga aacccttgcc acttagagac
     1741 tatcccacaa cctcccctct cccgttcggt acgagaggcg ccgggagtga ggggggctca
     1801 aatcgctgcc ggttttctcg ttcgactccg actatcgttt gccctcaccc caaccctctc
     1861 ccagagggag agggagctct cggataggct gttagagatt tcggctttca ggcataaacg
     1921 agaaccgacc ccctcgcctg gaaatggtct ctattgcgag gttccctaat ggaactttat
     1981 tacgaggctc cctaatggga cagagcagcg ccgtcgccgg ttggattagt ttccagcccg
     2041 gacaatggga aactgaacga ttgtcgcgct gtacggtgcg acctcgaacg aatccgcatg
     2101 ggaaacctcg ccgcgactgc gcagctcaat acgagggggt ttacccgggt cgttgtaatc
     2161 catcgggtca tttccggcca cgtcccagac tttaccactg ccaccgacgg tagcccctgc
     2221 cacgcgcaat gtgcagagtt gtttttcgcc ggtcggattc acgatcgcca cagcgagcgt
     2281 tttgttgtct ttcagaaggg ctgcctgtgc atcgagcagc ggattttgtg acgccgtc
//
LOCUS       DAOPTR010000667         2338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_36219_length_2338_cov_2.954008, whole genome shotgun sequence.
ACCESSION   DAOPTR010000667 DAOPTR010000000
VERSION     DAOPTR010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36219_length_2338_cov_2.954008"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2228)
                     /locus_tag="PKI05_11910"
     CDS             complement(<1..2228)
                     /locus_tag="PKI05_11910"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase family protein"
                     /protein_id="HOA52952.1"
                     /translation="MTCALRLAGDVWAFLWTAWTEVIANQVTIGGNKVVIQFARVLGL
                     AIVLWLAQYCAALVGEEGTQPTSPRGVLWQIGEVDKSPKGFALAPNQFAQYRGDAVFL
                     VGVSSPEKDWPYVQPGPLDVWAGSRTHQSTIIFSLEDAPLGPCELVLHLANTHGQHPP
                     RMTVAVNNKVVHEQVLPKGGPDPTITGDLSQAKPFEMRVPIAKEDLHSGTNTVTIASV
                     AGSWIIYDAVVFLAPPETRLSSPRDVTIVQRWESPPALIRHNGGLSQVVELTVLRGGE
                     PTNASVSYEGKICWTGPIAPGENEICVPVPRVSKSTQGTLTVEVGNQTIWRQAVLLEP
                     VREWEVYLLPHSHVDIGYTELQTKVEQDHWRYYEEALEAWNATATFPPEARFKWNVEV
                     LWAVDSYLRQASPERKEAFLQGVRDGAIGLQALYGNELTGLCRPEELLRLLDYAGRLE
                     ETYHFTIDSAMISDVPGYTWGIVTALAQAGVKYFSIGPNGGHRIGNTLRVWGDKAFWW
                     KGPDGGSRVLCWIPRRGYWRGFGGRDELMSYLKQLETEKYPFDMVQIRHCQGDNSGPD
                     TGISKFVKEWNEKYAFPKLIIATCSEMMRRFEARYGDVIPEVSGDFTPYWEDGAASSA
                     LETGLVRRAAEQLSMAEALWAMLRPSSYPDDRFYEAWRNVLLYDEHTWGAHNSITEPD
                     SAFAQGQWAIKRQFALDAARQAEELLTQSVASFGERNKGGRFLMVLNPCGWTRTDLVF
                     VAP"
BASE COUNT          548 a          719 c          569 g          502 t
ORIGIN      
        1 ggtgctacaa agacaaggtc cgtccgcgtc caaccgcacg ggttgaggac catcaggaac
       61 cgcccgcctt tgttgcgctc gccaaagcta gcgaccgatt gcgtcaacaa ctcttcggcc
      121 tgccgtgcgg catccagggc aaactgccgt tttatcgccc attgtccctg ggcaaaggca
      181 ctgtcgggtt ccgtgatgct attgtgggca ccccatgtgt gctcgtcgta aagaagtaca
      241 ttccgccagg cctcataaaa tcgatcgtca ggatagctcg aaggtcggag cattgcccac
      301 agggcctccg ccatcgaaag ctgctcggca gcacgacgga caagacctgt ttccaaagcc
      361 gacgacgctg ccccgtcctc ccaatagggc gtaaaatcac cgctaacctc tgggatgacg
      421 tccccatatc gggcttcaaa acgtcgcatc atctcggaac atgtggcaat aatcagcttg
      481 ggaaatgcat atttttcgtt ccattctttc acgaatttac tgattccggt gtctggccct
      541 gagttatcgc cctggcaatg gcggatttgg accatgtcga aaggatattt ttcggtttcc
      601 agttgtttca ggtaactcat cagttcgtcc ctacctccaa aacctcgcca gtaaccacgg
      661 cgaggaatcc agcagaggac acggctcccc ccgtctggcc ctttccacca aaaagcctta
      721 tcaccccaaa cacggagcgt gtttccgatg cggtgtccgc cgtttgggcc aatcgaaaag
      781 tacttgacgc ccgcctgggc cagcgccgtc acaattcccc aggtgtaacc cggcacgtcg
      841 ctgatcatgg ccgagtcaat ggtaaaatga tacgtctctt ccaatctccc cgcgtagtcg
      901 agtaatcgca aaagctcctc gggacggcac aaacccgtca gttcattccc gtaaagggcc
      961 tgcaggccaa tggcaccgtc gcgaacgccc tgtaaaaagg cctcttttct ttccggcgag
     1021 gcctgccgca gatagctatc aaccgcccaa aggacctcga cattccattt gaacctagcc
     1081 tccggcggaa aggtggccgt agcattccac gcctcaagag cttcctcgta atacctccag
     1141 tgatcctgct ccaccttggt ttgaagctcc gtgtacccga tatcaacatg ggaatgtgga
     1201 agaagataga cctcccattc cctcaccggc tccaaaagga ccgcctgacg ccaaatcgtt
     1261 tggttgccaa cttcgactgt gagcgtaccc tgcgttgatt tagaaacccg cgggacagga
     1321 acgcaaatct cgttttcgcc cggcgcaatt ggccccgtcc aacagatctt gccttcgtag
     1381 cttacagagg cattcgttgg ctctccgcca cggagaaccg ttaattcaac cacttgagaa
     1441 aggcccccat tatgccggat caacgccggt ggtgattccc acctttggac aatcgtgaca
     1501 tcacgaggcg agctcagtcg tgtttctgga ggagcgagaa aaacgaccgc gtcataaata
     1561 atccaactac ccgccaccga cgcaatcgta actgtgttcg tccctgaatg caggtcttcc
     1621 tttgcgatcg gcacgcgcat ctcaaacggt tttgcttgag aaaggtctcc ggtaatggtc
     1681 ggatcggggc cacccttggg tagtacctgt tcgtgaacca ctttgttatt gaccgcaaca
     1741 gtcatccgcg gtggatgttg accgtgggtg ttcgcaaggt gaagtaccaa ctcgcacggg
     1801 cccaggggag cgtcttccaa ggaaaaaatg atggtcgatt gatgcgtcct tgatcctgcc
     1861 caaacgtcca gcggacccgg ttgcacgtaa ggccagtctt tttcaggcga tgaaacaccc
     1921 acaagaaaga ctgcatcccc tcgatattgg gcaaattgat tgggagccag ggcgaaaccc
     1981 ttcggagatt tgtccacttc cccaatctgc caaagcacac cgcgcgggga agtaggctgt
     2041 gtgccctcct ctccgaccaa agccgcgcaa tactgagcca gccacagcac aatcgccaag
     2101 ccgagaacac gagcgaattg gatgaccacc ttgtttcctc cgatcgtaac ctgatttgca
     2161 atcacctcag tccacgccgt ccacaaaaat gcccacacat ccccagcaag ccgcagggca
     2221 cacgtcatta tgtgcatcca ccgtcggaca aacaatcatc agggtcgata cctgaactcc
     2281 gcgcgaggtc aacttcgatc atcggaaata actccgagac aaggccctcc tcacattt
//
LOCUS       DAOPTR010000668         2338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_36225_length_2338_cov_2.665353, whole genome shotgun sequence.
ACCESSION   DAOPTR010000668 DAOPTR010000000
VERSION     DAOPTR010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36225_length_2338_cov_2.665353"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PKI05_11915"
     CDS             <1..436
                     /locus_tag="PKI05_11915"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOA52953.1"
                     /translation="IFDSWAGCLSPKHYQQFVLPYTQIVFEAIPADVPAIHFLTGNPM
                     LLPLQAKAGGTVIGVDWRIDIGDAWDVVGDGYAIQGNLDPVVLCGPEELVVHEARAIL
                     QRVRGRPGHIFNLGHGVLPSTPVSNVSLLVQTVHEWNPRQIS"
     gene            653..>2338
                     /locus_tag="PKI05_11920"
     CDS             653..>2338
                     /locus_tag="PKI05_11920"
                     /inference="COORDINATES: protein motif:HMM:NF027656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4962 domain-containing protein"
                     /protein_id="HOA52954.1"
                     /translation="MRKLVFVCTLLIAVVSYLSTARGESAGLKLDERPTQPGEWGYRP
                     EDGTTSMVDPPAFRWRPQKEVENWEIEVARDREFQNIAYRVQDVIWNVHCPPRTLGPG
                     RYFWRYRGHGKGGPTQWSQVRSFTISANAVPYPMPTREDLLARIPKQHPRLFIRPEDL
                     PRLKQAATNEQREVWERLVQRCEQALRNPPPTEEPPKYPPDMQRKSDEWAKIWWGNRE
                     YTIRALGTSALLGFVYQVGGHRPYGELARKILMECAKWDPVGATGFRYNDEAGMPYAY
                     YFCRTYTFVYDLLSEEERQQCRQVMKIRGEEMYRTLCPRHFWQPYNSHANRAWHFLGE
                     VGVTFHQEIPEADDWTWFAMNVFFNTYPVWCDDDGGWHEGSSYWASYIQRFTWWADVM
                     RGTFGIDAYRKPYFSKAGYYAMYLMPPHGVSGGFGDLACTRESRSNVPLMSILAVQAR
                     NPYWMWYVESMGGAPEPREFWEFIRGTLPKVSSKPPVDLPTSRVFRGTGQAALNTTLL
                     DARENVQVLFKSSPFGLQSHGYDANNAFLLTAYNERLFVSSGRRDIYGSDHHAR"
BASE COUNT          501 a          578 c          696 g          563 t
ORIGIN      
        1 gattttcgat agctgggctg ggtgcctaag cccaaaacat tatcaacaat ttgtcttgcc
       61 ttatacgcaa atagtattcg aagccattcc cgccgacgtg cctgcaattc atttcctgac
      121 aggtaacccc atgttgcttc cactccaggc aaaggccggg gggaccgtca tcggtgtcga
      181 ttggcgcatc gacattggtg atgcctggga tgtggtaggg gacggttacg caatccaggg
      241 gaatctggac cctgtcgtgt tgtgtggccc agaggagctc gtcgtgcacg aggcgcgggc
      301 aatcttgcag cgtgttcggg gacgtcctgg gcacatcttt aacctaggcc atggtgtgtt
      361 accctcaacg ccggtcagta atgtctcgtt gcttgtccag accgttcacg agtggaaccc
      421 ccggcagata agttaggggt catgggcggt gaccagctat gatttggatt cgtggccact
      481 gtgtgcggtt tgtcctgtga tgtattgctg ttgtcttgaa acgaaggtcc ctgtccgtta
      541 gaacattcct acctgccatt ggtttattgg atgtctccgt ttgttcgggt tgttccagca
      601 ctggccaagc aagttgtctg tgttagcaag cgatgctagg gaggcatgtt ccatgcgaaa
      661 gttggttttt gtttgtactc tgttaattgc cgtggtcagt tatctcagta cggcgcgcgg
      721 agaaagtgca gggttgaagc tggatgagcg gccaactcag ccgggcgagt ggggatatcg
      781 gccggaagat ggcaccacgt ctatggtgga tcctccggca ttcaggtggc gaccgcaaaa
      841 agaggtggaa aactgggaga tcgaggttgc gcgggatcgt gaatttcaaa atatcgctta
      901 tcgggtccaa gatgtgattt ggaacgtgca ctgtcccccg cgaacgcttg gcccaggtcg
      961 gtatttctgg cgttaccgcg gtcatggcaa gggcggtcca acccagtgga gtcaagtgcg
     1021 gtcattcacg atttcggcca acgccgtgcc ttatccgatg cccacgcggg aagacctgct
     1081 ggcgagaatt cccaaacagc atccgcgatt gttcattcgt cctgaagacc tcccccgact
     1141 gaaacaggcg gctaccaacg agcaaaggga ggtgtgggaa agattggtcc agcggtgtga
     1201 acaggctctt cgcaatccgc cgccaacgga ggaaccgccc aaataccctc cagatatgca
     1261 acgaaaaagc gacgaatggg cgaagatttg gtggggaaat cgggaataca cgattcgtgc
     1321 cctaggcact tcggcactcc tgggatttgt ctatcaagtt ggcgggcatc gtccttatgg
     1381 ggagcttgcc agaaagattc tcatggagtg cgcgaagtgg gaccctgtag gggcaacggg
     1441 gttccgatac aacgacgagg ctggtatgcc ctatgcctac tatttttgcc ggacatacac
     1501 gtttgtttac gacttgctct cagaggagga acgccaacag tgtcggcaag tcatgaagat
     1561 acgtggcgag gagatgtatc gtaccttatg cccccgacac ttctggcaac cttacaacag
     1621 tcacgccaat cgagcatggc actttctggg cgaggtgggc gtgacttttc atcaagagat
     1681 tcccgaggcc gacgattgga cctggttcgc catgaacgtc ttcttcaata cctatccggt
     1741 gtggtgtgac gatgacggtg gttggcatga aggatcgagc tattgggcca gctacattca
     1801 acggtttaca tggtgggccg acgtgatgcg agggacattt ggaatcgatg cctatcgaaa
     1861 accctatttt tccaaggcgg ggtattatgc catgtacctc atgccacccc acggtgtgag
     1921 cggcggtttc ggtgatttgg catgtacccg cgaatcgcga agtaatgtcc ctttgatgtc
     1981 gattctggcg gtccaggcac ggaatcctta ctggatgtgg tatgtcgagt cgatgggggg
     2041 agcgcctgag cccagagagt tttgggaatt catccgcggg acgctgccca aagtttcgtc
     2101 aaagccgccg gtggatttgc caacatcgcg agtctttcgg gggacgggac aagccgctct
     2161 aaatacaaca cttctcgatg cccgcgaaaa cgtgcaggtc ctctttaagt cgagtccgtt
     2221 tgggcttcag tctcacggtt acgacgccaa caacgctttt ctgttaacgg cctacaatga
     2281 gaggttgttc gtgtccagcg gacgacgtga catttatgga agcgaccatc acgctcga
//
LOCUS       DAOPTR010000669         2332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_36324_length_2332_cov_3.579271, whole genome shotgun sequence.
ACCESSION   DAOPTR010000669 DAOPTR010000000
VERSION     DAOPTR010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36324_length_2332_cov_3.579271"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(725..798)
                     /locus_tag="PKI05_11925"
     tRNA            complement(725..798)
                     /locus_tag="PKI05_11925"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(762..764),aa:Val,seq:gac)
     gene            1252..2178
                     /gene="speB"
                     /locus_tag="PKI05_11930"
     CDS             1252..2178
                     /gene="speB"
                     /locus_tag="PKI05_11930"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOA52955.1"
                     /translation="MIPPRSHFQAGYNFLGLPDELADPQRAQVLILPVPYEATTTYGA
                     GTRNGPTAIIMASRQVELYDYRRGCEPASELGFATLPPLEIHSGSPTAMISRIEEVVL
                     HIFGGDPQPRFLVALGGEHSISAGIASAAARIFDPHEIVAVQIDAHADLRDEFEGSPY
                     NHACAARRILNCLPLMQIGIRNISEEEDRFRSTAHRLITIFADEWTEAAHERLAEFVR
                     NKLVYLTIDLDGLDPSIMPAVGTPEPGGLSWQQIQEVIRIIAQNARGVPIMDVVELAP
                     IPGLIAPDFLAAKLIYWAASDFLLREERKAAL"
BASE COUNT          501 a          669 c          657 g          505 t
ORIGIN      
        1 accaacccct tcgccaaggt cacacacacg gcagggaatc cccgggaacg gcggcgatat
       61 gtcacgctgc aagagacgca acgactgatc gacgcggccc ccaattggat ttggcggacg
      121 attattgcgc tctgtcggtt cggaggtcta cgctgcccta gcgaagtgct gtcgttgcgt
      181 tgggaggatg tggatttgcc gaatggccgg atgatcgtga cgtcccccaa gaccgaacac
      241 catccgggag gtggccgaag ggtagtaccg atcttcgtcc gactgcggcc ctacctggaa
      301 gaagcttggg accatgcgga aggacatact cacgtcatcc ccgaaaacct ctaccttaag
      361 gcaagccgcg gcccacgtgg ttgggtgaat tgcaacctcc ggacacaatt tgaacgcatc
      421 atccggcgag cgggcctgga accgtggccc cggttgttcc atagcttgcg ggcgtcatgc
      481 gaaaccgatt tggcggcaga gtatcccatc gccaccgttt gccgatggat tggaaacacg
      541 gttgccattg cggccaagca ctacgtggaa ccgcttgatg agcactttca acgggcagca
      601 ggcttggccc cactagcggc cccgcacacg ccagaaatcg gctgcaacga cgagaagccg
      661 aatcacgaaa accccgtttt ttccgcgttt tgcgctatat cgccgactag tgcaaatacc
      721 ccaatgggcg atgcgggact cgaacctgcg acccccagct tgtcgagctg gtgctctaac
      781 caactgagct aatcgcccaa cacactatat taatatcggt cgccgccgat ttggcaagcc
      841 agggtccttt tggcccagcg ctcctcgcaa cgacaggtcg catggcgaca acccttttgg
      901 gtggtttcaa agttgacccg attcgggatt caccttggga tcacggttaa tccattgaca
      961 ggcatgccac aacgtgtgac atggctcacc gttacgaatt cgcgatttca ttccggcagc
     1021 cgtctcgcaa taaaagcgct ttaacccgcc acgaggggcg caccgtgact gccgtgtggt
     1081 caaagacatg ggctgagccg tagcctcggc tcggcggtgg ctgccagatc ggttgtgggc
     1141 actttacaat gggatttccc accggagctc gagagaaaag caagcggttc ggggaagtac
     1201 gcagtggttt aaacattttt tgccaggact gtttgatata gagtcttatc gatgattcca
     1261 ccacgttcac acttccaggc gggctacaat tttttgggct tgcccgacga gttggcggat
     1321 ccccaacgcg cccaggtgtt gatcctgccg gtcccctacg aggcgacgac cacatacgga
     1381 gcgggcaccc gaaatgggcc gacggccatc atcatggcct ccaggcaggt ggagctgtac
     1441 gattatcgac gaggctgtga accggcgagc gaactaggtt tcgcgacgtt gcctccattg
     1501 gagattcaca gtggatcgcc caccgcgatg atttcccgga tcgaggaagt cgtcttacat
     1561 atttttgggg gagatcccca accgcgattt ctcgtagctc tcggtggaga acacagcatt
     1621 tccgcgggga ttgcgtcggc ggctgcaagg atctttgacc cccacgagat tgtggccgtt
     1681 caaatcgacg cccatgccga ccttcgcgac gagttcgagg gctctcccta caatcatgcg
     1741 tgtgcggcga ggagaatcct caactgtctt cccctgatgc agatcggcat tcgcaacatc
     1801 tccgaggagg aagaccgttt tcgaagtact gcccatcggc ttatcacgat cttcgccgac
     1861 gagtggacgg aggccgctca tgagcggttg gccgagttcg tccgcaacaa actggtctat
     1921 ttgacgattg acctcgatgg tcttgatccg tcgatcatgc cggccgtggg aaccccggaa
     1981 cccggtggtc tttcgtggca acaaatccag gaggttattc gcattattgc gcaaaacgcc
     2041 cgcggtgttc ctatcatgga cgtcgttgag ttagctccca tccccggctt gattgctccg
     2101 gactttttgg ccgccaagct catctactgg gcagcgtcag acttcttgct tcgggaagag
     2161 agaaaggctg ccctttgaac attagaagag ccaaaatgat gagcgattcg tgcccgattc
     2221 ccgcaaaacg aacccatttt gggcctttgc gcagattcgc cgagtcccct agtcttttaa
     2281 aagagggtcc ggcgatgacc acatgcttga gatgctctga aatgatgagt aa
//
LOCUS       DAOPTR010000670         2327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_36428_length_2327_cov_3.055018, whole genome shotgun sequence.
ACCESSION   DAOPTR010000670 DAOPTR010000000
VERSION     DAOPTR010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36428_length_2327_cov_3.055018"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PKI05_11935"
     CDS             complement(<1..193)
                     /locus_tag="PKI05_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52956.1"
                     /translation="MSLELIEVKLDFYYESIPKSLPLPLGEGWGEGKRLSESIEKTGS
                     DWGHPHPRPLSRRTGEGRLW"
     gene            complement(214..1722)
                     /locus_tag="PKI05_11940"
     CDS             complement(214..1722)
                     /locus_tag="PKI05_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOA52957.1"
                     /translation="MLRPRFHVLSAIVLLVTIFALPAGASDFQAEQGNLPHIVLILAD
                     DLGYGDLSCLNPDSKIQTPNLDQLAQEGMIFTDAHTPSSVCTPTRYGLLTGRYSWRTR
                     LQSGVLYGFDRPLLEPNRMTLASLLKSHGYATAAVGKWHLGLGWQSAQGTPVTDKDFT
                     TDDPGVDYTRPITEGPLTVGFDYFFGIAASLDMAPYCYIENDRVTAIPQEDTKGQEFP
                     MNWRPGKKSPDFKHDQVLSVLTERAMGWLSKTHKKAPKQPLFLYFALTAPHTPVLPLP
                     EFQGKSQAGSYGDFVVQVDHVVGQLVKTIEDLGIRDNTLVIFTSDNGSTMTIRPFFQK
                     YQHATNYHFRGQKSDIWEGGHRVPFLVRWPGHVPPKSVCHQPICLTDCLATFAELVSS
                     PLPVDAGEDSFSFLDLLRNPNAPFQRAPIVMHSIAGFFAIREGDWKLLECRGSGGWSL
                     PENKVPKDALPKQLYNLKDDVGEQHNLLLEHPDVAQRLSEHLEVIRTSGRSR"
     gene            complement(1921..>2327)
                     /locus_tag="PKI05_11945"
     CDS             complement(1921..>2327)
                     /locus_tag="PKI05_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52958.1"
                     /translation="AGTWAINVTGRSRYGIAWVLPPEKAIRIPIADKAEIRLPWIWIG
                     SSAASLKLELTDAPEGFAVVSLERDGGGAKLILSVDKDKIKPGTKGNLVFAVYGETQR
                     RGLVAASTQTVRYFVGWLPAVPFVAVEQKSSS"
BASE COUNT          537 a          643 c          629 g          518 t
ORIGIN      
        1 cccataatct cccctcgccc gttcggcggg agaggggtcg ggggtgaggg tgcccccaat
       61 cgctgccggt tttctcgatc gattccgaca atcgcttacc ctcaccccaa ccctctccca
      121 gagggagagg gagacttttg gggatagact cgtagtagaa atccagctta acctcgatca
      181 gttcgagcga caacgaatcc atcctggtca cgactagcgc gagcggcctg aggtgcggat
      241 cacttcaagg tgttcactaa gcctttgggc cacgtcggga tgttcgagca ggaggttgtg
      301 ttgttcccct acgtcgtcct tgagattgta tagttgcttt ggcaaggcat ctttggggac
      361 tttattctca ggcaagctcc agccgcctga gccacggcat tcgaggagtt tccaatctcc
      421 ttcgcgaatg gcaaaaaagc ccgcgatcga gtgcatcaca attggcgcac gctggaaggg
      481 cgcgttgggg tttctaagca aatcgagaaa actgaaactg tcctctcccg cgtcaactgg
      541 aagcggcgag gacacaagtt cggcaaacgt ggcaaggcaa tcggtgaggc aaattggctg
      601 atggcagaca ctcttagggg gaacatgacc tggccagcgg acaagaaacg gtacgcggtg
      661 gcctccttcc caaatgtccg acttttgtcc acgaaaatgg tagttggtgg catgctgata
      721 tttttgaaag aacgggcgaa tggtcatcgt ggaaccatta tcgctggtga agatgaccag
      781 tgtgttatca cgtatcccta aatcctcaat cgtcttcaca agctggccca cgacgtgatc
      841 cacttgaacg acaaagtctc cgtaactacc ggcttgactc tttccttgga actccggcaa
      901 cggtaacaca ggcgtgtgcg gggccgtcag ggcgaagtaa aggaaaagag gttgcttggg
      961 cgctttcttg tgtgtcttac tgagccagcc catggccctt tctgtcagaa cagataggac
     1021 ctgatcgtgc ttaaaatcgg gcgatttctt gccagggcgc cagttcatcg gaaactcttg
     1081 ccctttcgtg tcttcctgcg ggatggcagt aacgcggtca ttttcgatgt agcagtaggg
     1141 cgccatgtcg agagaagcag cgatcccaaa gaaataatca aaaccgaccg ttagcggtcc
     1201 ttctgtgata ggacgcgtgt aatctacgcc cggatcgtcg gtcgtaaaat ccttatcggt
     1261 gacaggagtc ccctgggccg attgccagcc aagccccaaa tgccatttac cgaccgctgc
     1321 cgtcgcgtat ccgtgcgatt ttaagagcga cgccaaggtc atgcggttcg gttcgagcaa
     1381 aggtcgatca aagccgtaca agactccgga ttgaagtcgc gttcgccatg aatatctccc
     1441 cgtcaatagc ccatatcggg tcggcgtgca caccgaggaa ggcgtgtggg cgtcggtgaa
     1501 aatcatgccc tcctgcgcca gttgatccaa gttcggtgtc tgaatcttag agtccggatt
     1561 caaacaggag agatcaccgt agcccaaatc gtctgccaaa ataaggacaa tatgaggcaa
     1621 attgccctgc tcggcttgga agtcactcgc cccggcggga agagcaaaga ttgtgaccaa
     1681 aaggacaatg gcactcaaaa cgtggaatcg tgggcgcagc attttcggcc tcctctagct
     1741 agtttgctct tgcccaaatc aatcgtttgc gaataacgcg gctagtcctt tccctgggtg
     1801 aggcattcgc tcctacagta ggaaatccaa ggacatttcc tacaggccga atcgccattt
     1861 tacaatcgga gaccggcagg aacaaacgta tcgaatcccg cccagcttag gaagcacccc
     1921 tcaagaactg gacttctgct cgaccgcaac aaacggcacc gccggtagcc atcctacgaa
     1981 ataccgaacc gtttgcgtgc tcgcggctac gagccctcgg cgttgggttt cgccatagac
     2041 tgcgaagaca agattgccct ttgtccccgg tttgattttg tccttatcca ctgaaaggat
     2101 caacttggct ccgccaccat ctcgctccag actgacgact gcaaaccctt ctggggcatc
     2161 ggtgagttcc aattttaggc tagccgcgga actcccgatc cagatccagg gaagacgaat
     2221 ctcggccttg tccgcaatcg ggattctgat ggccttctcc ggcggcaata cccaggcgat
     2281 gccataccgg gagcggcctg tcacattgat cgcccacgtt ccggcag
//
LOCUS       DAOPTR010000671         2325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_36464_length_2325_cov_3.759912, whole genome shotgun sequence.
ACCESSION   DAOPTR010000671 DAOPTR010000000
VERSION     DAOPTR010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36464_length_2325_cov_3.759912"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PKI05_11950"
     CDS             <1..878
                     /locus_tag="PKI05_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52959.1"
                     /translation="GDEGYVCHIQDVVTGAKRTISSPIYALAPDGHTAVTPDFRRIAN
                     LRPGYGYVGFPDPFAHERAPEQSGIWRVNLESGRSDLIISLAQAAAIRWPYADFGDAV
                     HWFNHLLVNPTGTRFVFLHRWKLPDEKSWRTRMFSSNMDGSELRVLIGSGFVSHFIWR
                     DETHVLAYSKPEPDAAWGFYLYEDVPNGRVEQVGAGVMGPGDGHCNYLPGNEWIVCDT
                     YPDKNRYQHVYLYHVPSNRRIELASLLSPAEYAGEWRCDTHPRVSRTGKFITVDSPHE
                     GGREIYLIDVSKIIG"
     gene            complement(1122..2312)
                     /locus_tag="PKI05_11955"
     CDS             complement(1122..2312)
                     /locus_tag="PKI05_11955"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOA52960.1"
                     /translation="MESHESVGIVVCLQPTGELKRRRGRFLRLTTTSGVLGILGILDQ
                     HDLPDSPAPDRLPKSPADSLLSLDDGTQEAFRLHEELRQSWRTVLHKGWPPLLVGSSA
                     TVRRLRQQIEALSKITLSVCIVTPPGGGGGLLARAVFHGAEPHPQQCLIPLPCEDLDP
                     VVLRMTLQVFDSVPPPPQEFPHTLLLENVDRLPNVSQEVLCHILASKPGSYRVLAVSN
                     QSLDQLVESGTFLPELARMIAHFTVLVPSLAERREDLPLMAQAVLEDLNVRGPKQVLG
                     FSREVLDLFAQYDWPRNFEELAWVIDQCYQATSQPIIDVDALPRELQWSMKHKMRLLR
                     DPETISLPTFLADVEKELLLRAMQRARGNKTRAAKMLGLSRARLYRRLLQHKLIKPED
                     LHAE"
BASE COUNT          518 a          603 c          668 g          536 t
ORIGIN      
        1 ggggtgacga gggctatgtg tgtcacattc aagatgttgt taccggcgct aagaggacga
       61 tttcttcacc catttacgct cttgccccgg acgggcacac ggcggtgacg cccgactttc
      121 gtcggatcgc gaaccttcgg ccaggttacg gttatgtggg gtttcccgat ccctttgcgc
      181 acgagcgggc accggagcaa tcggggattt ggcgggtaaa tttggaatct gggagatcgg
      241 acctcatcat ctcgttggcc caggccgcag ctatccggtg gccctatgcc gactttggtg
      301 atgcggtcca ttggtttaac catctcctgg ttaatcccac gggcacgcga tttgtctttc
      361 tgcatcgctg gaaacttccc gacgaaaaga gctggcggac acgaatgttt tcgtcaaaca
      421 tggacggaag cgagttgagg gttctgattg gctcgggatt tgtatcgcac tttatttggc
      481 gggatgaaac ccatgtgctt gcttattcga aacccgagcc ggatgccgcg tggggatttt
      541 acttgtacga agatgttccc aatggccggg ttgagcaggt cggcgccggg gttatggggc
      601 caggtgatgg acattgcaac tatctgccgg gaaacgagtg gatcgtatgc gacacctatc
      661 ccgacaagaa ccgatatcag catgtctatc tttaccacgt accttcaaat cgccggatcg
      721 agttggcgtc gttgttgtcg cccgcggagt atgcgggaga atggcgatgt gacactcacc
      781 cgcgggtaag tcggacaggc aagtttatca cggtcgattc accgcatgag ggtgggcgag
      841 agatttatct gatcgatgtg tcaaagatca tcggttaact gactccccga tatatcccta
      901 aaaggccccc tctccctctg ggagagggtt agggtgtggg cgagcggtcg tcggagtcga
      961 tcgagaaaac cggcagcgat gtgcgccacc ctcagccccg acccctctcc cgtcgaacgg
     1021 gtgagggaaa gattatgcaa tactctaaat gaccttttac gacctgctac gtgtttccgg
     1081 tggtttactt gtgggaggct ccggaatcgg tctgcaggta tttactcagc gtgcagatct
     1141 tccggcttaa tgagcttgtg ttgaaggagc cggcgataga gcctggcacg ggaaagcccc
     1201 agcattttgg cagcccgtgt cttgttgccc cgtgcacgct gcatagcgcg aagcaagagc
     1261 tctttttcca catcggcaag aaacgtaggc aaggagatcg tttccggatc gcggagaagt
     1321 cgcattttgt gtttcatgga ccattgcagc tcgcgtggca aggcgtcaac atcaatgatc
     1381 ggttgagagg ttgcttggta gcactggtcg atcacccatg caagttcctc aaagttccgc
     1441 ggccagtcgt attgagcgaa caaatccaag acttccctgg aaaaacccaa aacctgcttg
     1501 ggccccctga cattgaggtc ttctaggacc gcctgggcca tgagcggaag gtcctctcgc
     1561 ctttcagcca agctagggac taaaacggtg aagtgtgcaa tcatccgggc gagttcgggc
     1621 aaaaaggttc cggattcaac caattgatca agcgactggt tgctgacggc taatacccga
     1681 tagctccctg gctttgaggc cagaatgtga cacaaaacct cctgggaaac gttcggaagt
     1741 cggtcaacgt tttccagcag caaagtgtga ggaaactctt gtggcggagg tggtactgaa
     1801 tcgaaaactt gaagcgtcat cctcaagacg acagggtcga ggtcttcgca cggaagcgga
     1861 atcaaacact gctgcgggtg aggttccgct ccatggaaaa ctgccctggc caaaaggccc
     1921 ccaccaccac ccggcggcgt gacaatacaa acgcttaaag taatcttaga cagagcttcg
     1981 atctgttgac gcaaccgacg aacggttgcg cttgaaccaa caaggagggg cggccaacct
     2041 ttatgcagga ctgtcctcca actttgccgt aattcctcat gtagccgaaa tgcctcctga
     2101 gttccgtcgt ctagcgatag taatgagtcc gctggacttt ttggcaatcg atccggtgca
     2161 ggtgaatctg gcaaatcatg ctggtcgagt ataccgagaa tgcccaagac accggacgtt
     2221 gtggtgagtc tcaggaatct tccccgacgt cttttcaatt ctcctgtggg ctgaagacac
     2281 acgacgatgc cgacactctc atgggactcc attgccccgg gcgga
//
LOCUS       DAOPTR010000672         2323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_36504_length_2323_cov_3.174162, whole genome shotgun sequence.
ACCESSION   DAOPTR010000672 DAOPTR010000000
VERSION     DAOPTR010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36504_length_2323_cov_3.174162"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PKI05_11960"
     CDS             <1..509
                     /locus_tag="PKI05_11960"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HOA52961.1"
                     /translation="FFRGALRRWAEVLVSSGVLARSVLPALASVTGLVFYSWMRFLWP
                     NRKQSIGAWQRLSHRSDLPADSVDGRWVTSHGVWIVRRTDQGRDRLFVLRAACTHLGC
                     TTQWDPSLRQFICPCHGSAFSLDGERLRGPATRAMDRYAVRLTSDGFIEVRLESTVRR
                     PQSEIEYS"
     gene            521..1294
                     /locus_tag="PKI05_11965"
     CDS             521..1294
                     /locus_tag="PKI05_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HOA52962.1"
                     /translation="MSWLAKLRQSRVYQSIFRVPIPKDRRHKVLVILSTFVLHVHPVT
                     IPRYALRLRHTWCMGGLAFFLFLVEVVTGVLLMFYYRPVPGMAYQDMWELRSSVTFGI
                     FREVHRWAAHAMVIVVILHMLRVFLTGSYRPPREFNWVIGVALLVLTLLLSFTGYLLP
                     WDQLSLWAATVGTNMVAASPFVGCEGPGSQWITWHGSPLITRQSDLRYFLLGSSQIDD
                     VALNRFYVWHCIGLPILMTFLCALHFWRVRKDGRISGPL"
     gene            1371..>2323
                     /locus_tag="PKI05_11970"
     CDS             1371..>2323
                     /locus_tag="PKI05_11970"
                     /inference="COORDINATES: protein motif:HMM:NF012262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52963.1"
                     /translation="MNLLPIVICYLLLALSSTIALAVILRRRSPPRRRVSDFVDIAAL
                     GSYAVLGVLGALSTAVGHPPGLPSAVKDALNWLLTPATVMTLWLLAMVILLWRPSIFS
                     SPWIFWHAATWGSLWFLLSMTDSHFAALVLKPDHIAVVFLVALTGFFTWLGLRQSVLN
                     DQRLDRGRKPREATKPEQTRVFVWPDLVYIELIAILGMTCVLGVWSLAVPAPLEAPAD
                     PAWTPNPAKAPWYFVGLQELLVYFEPWFAGVIVPVIITVGLCLIPYLRRRDQRSGYYS
                     LKGRRFSVAIFLFGLGLWVLLILIGTFFRGPDWRLYGLYESR"
BASE COUNT          381 a          599 c          697 g          646 t
ORIGIN      
        1 ccttttttag aggggcactc cggcgatggg cggaggtgct ggtgagctct ggagttttgg
       61 ccaggtccgt gcttcctgcc ctggcctcgg taaccgggct tgttttttat tcctggatgc
      121 gttttttgtg gccgaatcgt aagcagtcca tcggggcctg gcagcgtctc tctcacagga
      181 gtgacttgcc cgccgattcg gtggatggcc gctgggtgac cagtcatggg gtctggatcg
      241 tccgcagaac cgatcaaggc cgagaccggc tgtttgttct tcgagctgct tgtacccatt
      301 tgggttgtac aacacagtgg gatccgtcgt tacggcagtt tatttgtccg tgccacggaa
      361 gtgccttctc gttagatggg gaacgtctcc gtgggccggc tacccgggcg atggatcgct
      421 atgccgttcg gttgacaagt gatggcttta tagaggtacg actggagtcc actgtgcgaa
      481 ggccacaaag cgaaattgag tattcctagg ggaattccgg atgtcttggc tggccaaatt
      541 acgtcagagc cgagtttacc aaagcatctt tcgcgtcccc atcccgaaag accggcgtca
      601 taaggtcctt gtgatccttt cgacgtttgt gttacacgtc caccccgtca ccattcctcg
      661 gtatgcattg cgtttgcggc atacctggtg tatgggcggg ctggcgtttt tcttgtttct
      721 cgtcgaggtg gttaccggcg tgctgcttat gttttactac cggcctgtcc cggggatggc
      781 ctaccaggac atgtgggaac tgcggagctc ggtgaccttc gggatcttcc gagaggtcca
      841 ccgctgggct gcccacgcca tggtcattgt agtcatcctg catatgttgc gagtcttctt
      901 gacaggaagt tatcgaccgc cgcgggaatt taactgggtc atcggtgttg ctttgctggt
      961 actgacgtta ttactctcgt tcacgggtta tcttcttccc tgggaccaat tatcactttg
     1021 ggccgccacg gtggggacga acatggtggc ggcatccccc tttgtcggtt gtgaggggcc
     1081 agggagccaa tggattacgt ggcacggctc gcctcttatc acacgtcagt cagatttgcg
     1141 gtacttcctt cttggcagta gccaaatcga tgacgtggcc ttaaatcgtt tttatgtgtg
     1201 gcattgcatt ggtctgccca ttttgatgac gttcctgtgt gcgttacact tttggcgggt
     1261 gagaaaagac gggcggatca gcggacccct gtgaggcgga ctgcccgtga accaagccgc
     1321 ggtccgaaaa gccggcctct tgggtcgcga atcatcctga ccgagacatc atgaacctgt
     1381 taccgattgt gatctgttat ttgctgcttg cgctgagtag cacaattgcc ttggcggtta
     1441 ttcttcgtcg gcgatcgccg ccacgccggc gagtatcgga cttcgtcgat atcgcggcgc
     1501 tgggcagcta cgctgttttg ggagtgcttg gtgcgctgtc aacggcggtg ggacatcctc
     1561 cgggtttacc gtccgccgtg aaagacgctt taaattggct gctgactccg gccacggtaa
     1621 tgaccttatg gctgctggcc atggtcatcc tcttgtggag accaagtatt ttctcttcgc
     1681 cctggatctt ttggcacgct gccacgtggg gtagtctgtg gtttttactg tcgatgacgg
     1741 acagccactt tgcggcactc gttctcaagc cggatcacat tgccgtggtc tttttggtcg
     1801 cactgacagg cttcttcacc tggctagggc tgcggcaaag tgttctcaat gaccaacggt
     1861 tagatcgtgg ccggaaacca cgagaggcga caaaaccgga acaaacacgg gtttttgtgt
     1921 ggcctgacct cgtgtatatc gaactcatcg ccatcctggg gatgacatgc gttttggggg
     1981 tgtggtcttt ggccgtcccg gcacccttgg aagccccggc ggaccctgcg tggacgccca
     2041 atcctgccaa agccccctgg tattttgtgg ggcttcagga attgctggtc tattttgagc
     2101 cgtggtttgc gggcgtcatt gtgccggtta tcatcacagt gggcttgtgt ttgattccct
     2161 atctgcggcg tcgcgatcag cgatcagggt attactcgct gaagggccgc cgcttttccg
     2221 tggcgatctt tcttttcggt cttggtctgt gggtgttgct gattctgatt ggaacatttt
     2281 ttcgcggacc tgactggcgt ttgtatggac tgtacgaatc gcg
//
LOCUS       DAOPTR010000673         2319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_36579_length_2319_cov_3.651943, whole genome shotgun sequence.
ACCESSION   DAOPTR010000673 DAOPTR010000000
VERSION     DAOPTR010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36579_length_2319_cov_3.651943"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PKI05_11975"
     CDS             complement(<1..260)
                     /locus_tag="PKI05_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52964.1"
                     /translation="MSTFDPADVLFHVPTLSDAERLVIAGLFPDVLLLLEDHPRQFSE
                     QDWLSFMAHSPLTRAVAIYTDWCESEPRTGSPLPGVIRISWLR"
     gene            701..937
                     /gene="csrA"
                     /locus_tag="PKI05_11980"
     CDS             701..937
                     /gene="csrA"
                     /locus_tag="PKI05_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HOA52965.1"
                     /translation="MLVLSRQRDESIIIGDNIVVTVVDIRGDKVRLGIQAPPEITVHR
                     REVYEAIQREKKMKESQQANPSVQPEVSGQKETT"
     gene            1356..>2319
                     /locus_tag="PKI05_11985"
     CDS             1356..>2319
                     /locus_tag="PKI05_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOA52966.1"
                     /translation="MTRINTNITSLNAQKTLARSQAQLQEALTRLSTGLRINVGKDDP
                     AGLIASEALRADITAVQKAISNSERANQMIATADSALGQISQLLNDIRGLVSEAANKG
                     AMSAEQIAANQLQIDSSLEAIDRIAKVTQFQGQRLLDGSFDFITTGVDTSKITSLRID
                     QANFGALSSISVNVDIVSQASKAKLTYNYGALATDVVLEIAGKDGAEAFSFAAGSSIE
                     DMAQAINLVSDALGVRAVVHQEATAGSLTASSFGSNNDVIITANEAGQAAGDLRIKYT
                     KSSSATTSVVYTPASGNTPGLIEVQLGTRQWEAASGVLDGTGTNN"
BASE COUNT          583 a          561 c          684 g          491 t
ORIGIN      
        1 cgaagccaag agatgcgaat gacccccggt agcggtgaac ccgtccgagg ttcgctttcg
       61 caccagtcgg tatagatagc caccgcgcgg gtcaaaggcg aatgggccat gaaggaaagc
      121 cagtcttgtt cggagaactg gcggggatga tcttccaaca agaggagaac atcggggaaa
      181 agaccagcga tgaccagccg ttcagcgtcg ctaagcgtcg gcacgtgaaa caatacatcg
      241 gcgggatcga aggtggacaa gaagagactc cattcgggac gacgatcatc gccaacaatg
      301 ctgacggcga gtctctgtgc tgggcgagat gggggtggtt tcatactacc caagaaactc
      361 tcttaggcaa ataatgccgc aaaaacgacc taggggattt cgactactgc cggagaacgt
      421 ctgtcggaca tgaatacctt gtgatgttat ccttcacggg tggcggagac aaggtgcatc
      481 gatctcccac ggctctaacg gaatattcga tataatcgtc gctttgtgga gaggatttag
      541 tctgtgtacc atttgtgccg aagcgattac tcagaggtgg atactgtcat tgttgttgat
      601 ggatagagaa tcggcgtggc caacgaagga cgaagccctc cccgatgatg gcatgtggtt
      661 ccggggagca ggatcatagg caaaatgaag ggagtgtacg atgcttgtcc tttctcgcca
      721 gcgagacgag agtatcatca tcggggacaa catcgtcgtg acggtggtgg atattcgcgg
      781 ggataaagtg cggttaggga ttcaagctcc gccggaaatc acggtacatc gtcgcgaggt
      841 gtatgaagcg attcagcgcg aaaagaagat gaaggaaagc cagcaggcta atccttcggt
      901 tcaacctgaa gtctcaggtc agaaagaaac tacgtagcca gtgttcagat tgtgacgctt
      961 gtactaaacg aacggccacg aacgggaaaa tccctcacgg ttcgtggccg ctttcgtttt
     1021 tgtgacctac agttttatgc cgaagcgaaa aaaaagaggg attgacgagg gtttagcagg
     1081 ttaacgcagg ttttgtaaag ttcccaactt acccaaacga tatccctcaa aggacggatt
     1141 gatccagcca gagcgttttg ccgcaagcgt cggcagtggc ccacggaagg tgatcagtgc
     1201 gagctggatc gaggccgagg ttcggtcaaa gtcgggtaac ggttcgattg tctaacccgc
     1261 gcaactataa ggacagcgaa tcggatcccg tggttagatt ggtcccgaaa agagtttggc
     1321 ttggtcctag ctattcagaa tgaagggtaa cagacatgac ccggattaac acgaacatca
     1381 cctcgttgaa cgctcagaag acgttggctc gttcacaggc tcaattgcag gaagccttga
     1441 cccgattgag caccggcctg cggatcaatg tcggtaaaga tgacccggca ggcttgattg
     1501 ccagtgaggc cttgcgggcg gacatcaccg cggtccaaaa ggcgatctcc aacagcgaac
     1561 gtgcaaatca gatgatcgcc acggcggaca gcgctttggg tcagattagc cagcttctca
     1621 acgatatccg gggtctggtc tctgaggcgg ccaacaaagg cgccatgagt gccgagcaga
     1681 tcgccgcgaa tcagttgcag attgattcct ctctggaagc cattgaccga atcgccaagg
     1741 tcacccaatt ccaagggcaa cggctcctag atggcagttt tgatttcatc accacgggtg
     1801 tggatacatc caagattacc agtttgcgca tcgatcaggc caactttggt gctctgagca
     1861 gcatttccgt gaatgtggat attgtctcgc aagcttcgaa agccaaactg acatacaact
     1921 acggtgcttt ggccaccgat gtggtcctcg agatcgccgg gaaggacggg gccgaggcct
     1981 tcagctttgc agcgggtagc tccatcgagg atatggccca ggccattaac cttgtctcag
     2041 acgccctcgg tgttcgcgct gttgttcatc aagaagcaac ggcgggtagt ctcacagctt
     2101 cgagcttcgg ctccaataat gacgtgatca ttacagcgaa cgaggcaggg caggcggctg
     2161 gtgacctgcg gatcaagtac accaagagct cttctgcgac cactagtgtc gtctatacac
     2221 ccgccagtgg caatacgcct ggtctaatcg aggttcaatt aggtactcgg cagtgggagg
     2281 cggcgagtgg ggtgttggat ggtacgggga cgaacaatg
//
LOCUS       DAOPTR010000674         2308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_36795_length_2308_cov_2.800266, whole genome shotgun sequence.
ACCESSION   DAOPTR010000674 DAOPTR010000000
VERSION     DAOPTR010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36795_length_2308_cov_2.800266"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /gene="leuD"
                     /locus_tag="PKI05_11990"
     CDS             <1..526
                     /gene="leuD"
                     /locus_tag="PKI05_11990"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOA52967.1"
                     /translation="IIPKQFLKRIERTGFGQFLFYDWRFREDGTPNPEFILNAPQYQG
                     ATILLARRNFGCGSSREHAPWALADYGFRALIAPSFADIFYNNCLKNGLLPVRLDENL
                     VDDLFRRCREKPHYQLTIDLEACLVTDDFGFRASFDIDPFRRKCLLEGLDDIALTLQH
                     EDKILAYEKARGIV"
     gene            782..1357
                     /locus_tag="PKI05_11995"
     CDS             782..1357
                     /locus_tag="PKI05_11995"
                     /inference="COORDINATES: protein motif:HMM:NF022273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HOA52968.1"
                     /translation="MVLWDAVRIFFATLWDKRVAEGVRSLLRGGQVAEPQRPSVEPDR
                     PVTVAPPKPPLAKPREKLTKSEALILLETLQREARFVDFIKEDLSGYSDAQIGAAVRD
                     IHRDCAAVLERLFAIRPVVTATEGADFELPHDFDSGRFRIVGNVVGSGPYHGKLVHHG
                     WEATTCQLPLWQGSESAVRIIAPAEVEVVGQ"
     gene            1485..>2308
                     /locus_tag="PKI05_12000"
     CDS             1485..>2308
                     /locus_tag="PKI05_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOA52969.1"
                     /translation="MKPTFVIGIDLGTTNLVLSFTPYDADEAHPQILEIPQLVAPGTI
                     ERRTTLPSFLYLARDHEIASRSLDLPWASDQTFAVGEFARRQAAESPERTISAAKSWL
                     CYSRVDRRQPILPWGAPPEVPRISPVTAAQRYLEHLIAAWHHQFPDAPIRQQRVVLTV
                     PASFDAAARELTREAALAAGLPEDLILLEEPQAALYAWLADTGPRWRKLLKVGDQVLV
                     CDVGGGTTDFTLVAVAERDGELALERLAVGNHLLVGGDNMDLAMSHLVSQYFAEKG"
BASE COUNT          476 a          629 c          690 g          513 t
ORIGIN      
        1 aattattccc aagcagttcc tcaagcgaat tgagcgaact gggttcggac agttcttgtt
       61 ctacgactgg cgcttccggg aggatggaac ccccaatccg gaattcatcc ttaatgcccc
      121 acagtatcaa ggtgccacca tcttgttggc gcggcggaat tttggctgcg ggtccagtcg
      181 tgaacacgcc ccctgggccc tggcggacta tggctttcgg gcactgattg cgcccagttt
      241 tgccgacatt ttctacaaca attgcctcaa gaacgggcta ttgcccgtgc ggctggacga
      301 aaatcttgtc gatgatttgt ttcgacgctg ccgagaaaag ccgcattatc agttgacgat
      361 tgacttggaa gcctgcctgg taacggacga ctttggtttc cgcgcgagct tcgatatcga
      421 tccttttcgg cggaaatgtc tgctcgaggg cctggacgat attgccctga cgttacagca
      481 tgaggacaaa atcctcgctt atgaaaaggc ccgggggatc gtgtgatccc ctccgctggg
      541 caaaccgaac tatgatgccc acgagaggac aaaaaacaga ttcgccgggc caagtggctt
      601 tcccaaaagg cccgagtgtc ccacgactcc ggtctttcgg tagaattgac ggtagcgtca
      661 aaggttggcc gaagaaccgc tggccaatga gggcttgccg tagcgaggtg ttgcgttggg
      721 aaaaaccgtc cggcaacccc ttacatgggc aaatttccgc tagtaagtac aggagcctcc
      781 gatggttttg tgggatgccg tccgaatatt tttcgcgacg ttgtgggata aacgggtggc
      841 tgaaggcgtc cgctccctcc ttcgtggtgg acaagttgcg gagccgcaac gccccagtgt
      901 ggagccagat cgccctgtca ccgttgcacc accaaaaccg ccgttggcaa agccccgtga
      961 aaaactgacc aagagcgaag cgctgatcct cctggaaacc ctgcaacggg aagcccgctt
     1021 tgtggacttc attaaggagg acctcagtgg atactcggat gcccagattg gtgccgctgt
     1081 ccgggatatc caccgagatt gtgccgctgt gttggaacgg ttgtttgcca ttcgccccgt
     1141 agtaacggcc acggaagggg ctgattttga actgccacac gatttcgact cgggccgttt
     1201 tcgcatcgtg ggaaatgttg tcggctctgg accctaccac ggaaaactgg ttcatcacgg
     1261 ctgggaggcc acaacttgtc agttgcctct ttggcaaggt agcgaatctg ccgtcaggat
     1321 cattgcaccc gccgaggtgg aagtcgtcgg ccagtagctc tgtggctggt ccacgttagg
     1381 taaccgtgcc tccgccacga ccgtgtgtga ggtttggttc ggcctaaccg aagagctgtg
     1441 ccgcttgcaa gcgaacaacg tctcaagagg gggagttacg aagaatgaaa ccaacgtttg
     1501 tcattgggat tgacctggga acaactaact tggtgttgag cttcactccg tacgatgcgg
     1561 atgaggctca cccacagatt ctggagatac ctcagcttgt ggcgccgggt accatcgaac
     1621 ggcggacaac gctgccgtcg tttctctatt tggcgcggga ccatgagatt gcgtcgcgga
     1681 gtctcgacct gccctgggcg tccgatcaga cgttcgccgt gggggaattt gctcggcgac
     1741 aagcggcgga gtctcctgaa cggaccatca gtgcggcgaa atcatggttg tgctacagtc
     1801 gcgtggatcg acggcagccc atccttccct ggggggcacc accggaggtc ccccgcatct
     1861 cgcccgtaac ggccgctcag cggtatctgg agcacctcat agcggcttgg caccatcagt
     1921 ttccggatgc tcccattcga cagcagcgag ttgtgttgac agttccggca tcttttgacg
     1981 ccgcagccag agagctgacc cgcgaagcag ccctcgccgc gggattgccg gaggacttga
     2041 tcctcttgga agaaccgcaa gcagcccttt acgcctggct tgccgataca ggtccgcgat
     2101 ggcggaaact gcttaaggtg ggcgatcaag ttttagtgtg tgacgtgggt ggagggacaa
     2161 cagatttcac gctcgtggcc gtggccgaac gggatggtga attagcactg gaaaggctgg
     2221 ctgttggcaa tcatcttctc gtcggtggtg acaacatgga cctggcgatg tcccatctgg
     2281 tcagccagta ctttgccgag aaggggat
//
LOCUS       DAOPTR010000675         2306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_36828_length_2306_cov_3.671701, whole genome shotgun sequence.
ACCESSION   DAOPTR010000675 DAOPTR010000000
VERSION     DAOPTR010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36828_length_2306_cov_3.671701"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="PKI05_12005"
     CDS             <1..597
                     /locus_tag="PKI05_12005"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52970.1"
                     /translation="VHLAFSNSENVHPEKNVVLRFGRRYLPISKVTIFDRFFIREAGR
                     LCATPAFLVLLVVESTDVLFAVDSIPAVIGITRDPFIVFSSNIWAILGLRALYFLLVG
                     VLDRFHYLHYGLAAVLSFIGLKMVAEYGAHHWFEYHERLINPWVSLAVVGGLLGLSLI
                     PTFLGYKARSSDLTSEAEDESREGQQRELAKVHDSETR"
     gene            637..987
                     /locus_tag="PKI05_12010"
     CDS             637..987
                     /locus_tag="PKI05_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52971.1"
                     /translation="MSDRSGEIVWRRVFHCPTGLETRSQVWLVIEGPRQPQCVRLNGD
                     RLFDAGAPPETPHKSPLRLEIQTRLKPTKNVLEITFPGHGQFSELDHQMDGQLLQPIP
                     LEVYLEIQDSESKN"
     gene            1093..>2306
                     /locus_tag="PKI05_12015"
     CDS             1093..>2306
                     /locus_tag="PKI05_12015"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOA52972.1"
                     /translation="MISKPDMFKVGVLIIVGMCTLVVSGVAGAEETSSVSSIDVNGPA
                     LLALFESTLVDVIARTEKSVVAIARVRTSRPGEVFNLEPRLDPFGGLAERSITPQTGD
                     PNFFPTDYGAGVVVDSKGLILTVAQLIGEESEYYVTTCDRRTFRARVVAADPRSDLAV
                     LSVPAEDLQPITLGNAALLKKGQIVIALGNPYAIAKDGQVCASWGIVSNLGRKAPPMP
                     TDRDPTGKPTIHHFGTLIQTDAKLGFSSNGGALVNLKGEMVGLLTTLPVATGYETPAG
                     YAYPVDETFLRVLNHLKRGEEVEYGLLGIQPESLSLAERASGLQGTRIDRVVPGTPAD
                     RVGLRGRDLLVAVNGEPLYDDDGLVLAVGRLPVDSKVTLDVVRDRRRFQVQTVLTKYP
                     VRGRKIVTAPPQ"
BASE COUNT          470 a          574 c          678 g          584 t
ORIGIN      
        1 gttcaccttg cgttttccaa ttcagagaat gtccatccgg aaaagaatgt cgtcttacga
       61 tttggtcgcc gttatctgcc gattagcaaa gtcactattt tcgatcgttt tttcattcga
      121 gaggccggcc gactgtgcgc cacgccggct tttctggtgc tgcttgtcgt cgaaagcacg
      181 gacgttttgt tcgccgtcga tagcattccc gctgtcatcg gaattacgcg tgatccgttt
      241 attgtttttt cctcgaacat ctgggccatc cttggcttgc gggcgttgta ttttctcctg
      301 gtgggcgttc tggaccggtt tcattacctt cattacggcc tggcagcggt gctttcgttc
      361 atcggcctga agatggtggc cgagtacggc gcgcatcact ggtttgaata ccatgaacgc
      421 ctgatcaacc cttgggtatc ccttgcggtt gttgggggat tgttgggttt gtctttgatc
      481 cccacattcc tgggttataa agcccgatcc tccgacttaa catccgaagc cgaagatgaa
      541 agtcgagagg gacagcaacg cgaattggcc aaagtacacg attcggagac tcgttagttg
      601 gtccatcgga ttcggttgac atcagcgtgg acagtcttga gcgatcgttc gggcgaaatc
      661 gtctggcggc gggtgtttca ttgccctaca ggcctggaaa cccgctcgca agtgtggctt
      721 gtgattgagg gacctcggca accgcagtgt gtacgactta atggtgatcg actgtttgat
      781 gctggggccc ctcctgaaac tccgcataag tcgccgctga ggcttgagat tcagacccgt
      841 ttgaagccga ccaaaaatgt tttggaaatc acttttccag gtcatgggca attttcggaa
      901 ttggaccacc aaatggatgg gcagttgttg cagcctatcc cgctggaggt ttatcttgaa
      961 atacaagata gcgagtcaaa gaactaagga actttttgtt tatcttgggg catacccgaa
     1021 tcgtcattaa aaacactggt tttgatgttg gcgtgaaggg gaaattggct caatcgcgag
     1081 gcgaggtgtg ccgtgatttc aaaacctgac atgtttaagg tgggtgtgct tatcatcgtg
     1141 ggcatgtgca cccttgtcgt ttccggtgtt gccggtgccg aagagacttc gtcagtgtcc
     1201 agcattgacg taaacggccc tgccttgctc gccctgttcg aatcaacgct cgtagatgtc
     1261 attgcgcgga cagaaaaatc ggtggtggcg attgcccggg ttcgcaccag ccggccaggg
     1321 gaggttttca atttggaacc gcgattggat ccatttgggg gcctggccga acggagtatc
     1381 acaccgcaaa ccggcgaccc aaatttcttt cccacggact acggggcggg cgtggttgtg
     1441 gactccaaag gtttgatcct tactgtggca caactcattg gtgaggaaag tgaatactac
     1501 gtgacgacct gcgaccggcg gacgtttcgg gcgcgcgttg tggcggccga tccgcgcagt
     1561 gatctggccg tactttccgt tccggcagag gatctgcagc cgattacgct ggggaatgct
     1621 gccctcttga aaaaaggcca aatcgtcatc gcattaggca atccttatgc cattgcaaag
     1681 gacggtcagg tgtgcgcaag ttgggggatt gtgtcgaacc tcggtcgcaa ggcgccgcca
     1741 atgcccacag atcgggatcc caccggcaag cctaccatcc atcattttgg gaccctgatt
     1801 caaacggacg ccaagttggg cttctccagt aacggtggtg cgttagtgaa tttgaagggg
     1861 gaaatggtgg gactgttaac cacgcttcct gtcgccacag gttacgaaac ccccgcgggt
     1921 tatgcctatc cggtggatga gaccttcctc cgagttctaa accacctgaa acgcggggaa
     1981 gaggttgaat acggtttgct cggcattcag ccagaaagtt tgtcgctggc tgagagggca
     2041 agcggtcttc aagggacgcg gattgatcgg gtggtaccgg gaacacctgc ggaccgcgtc
     2101 ggcctgcgcg gtcgggatct cctcgtggcg gtcaacggtg agcctctcta cgatgacgat
     2161 gggttggtac tggcggtggg gaggttgccc gttgactcaa aggtcacact ggatgtcgtc
     2221 cgtgaccggc gtcgctttca ggttcagact gtcttaacga agtatccagt tcgtggccga
     2281 aagattgtga cagcgccgcc ccaaaa
//
LOCUS       DAOPTR010000676         2299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_36965_length_2299_cov_3.024955, whole genome shotgun sequence.
ACCESSION   DAOPTR010000676 DAOPTR010000000
VERSION     DAOPTR010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36965_length_2299_cov_3.024955"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="PKI05_12020"
     CDS             <1..738
                     /locus_tag="PKI05_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOA52973.1"
                     /translation="VPRDHMFAFPEDVGGRFSVLSAVGLVPAAILGLNIEELLEGACA
                     ANRAFSNSDPASNPILRYVGVCHLMESRRGASIRVLSTWGKRLEAVGLWYDQLLSESL
                     GKNGQGATPLTVVNTRDLHSRGQQHQEGRRDKLFTNLWAERPSLACVTIPRWDYDDDQ
                     LNKLAGKSLSDILRAARLGTTQAYAEDLRRSAEIILPQIRERTLGELFQFLMLATVVE
                     GRLIGINPYGQPGVEAYKRHMQKLLEQ"
     gene            857..1543
                     /locus_tag="PKI05_12025"
     CDS             857..1543
                     /locus_tag="PKI05_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOA52974.1"
                     /translation="MATQLRWLGHGCWLIETNGHRIILDPFLDESPVAPIKADEVKAD
                     YVLISHGHFDHMADAESIAKANAATVIATYEICEWLNRRGVTSTHAMNVGGTHAFPFG
                     RVKMTPAVHTSMLPDGSYGGVAVGFLLLLQDGNVYFACDTGLFLDMQLIGDHDLALAV
                     LPIGDNFTMGPEDALRAVTLLRPKRVVPVHFNTWPLIAQDVNDWANRVRHTTASVPVV
                     LQPGESIALG"
     gene            complement(1632..1970)
                     /gene="nirD"
                     /locus_tag="PKI05_12030"
     CDS             complement(1632..1970)
                     /gene="nirD"
                     /locus_tag="PKI05_12030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HOA52975.1"
                     /translation="MDLFDWQSSGEWIPVCKLDDLQKNKGKRFVVAGREIAIFRTENG
                     VFATDDYCPHMGDSLSAGRIWEGTVVCPRHMWAFRLADGRCTDVPTLSVPTYKVTVRD
                     GWVLLKKPVE"
BASE COUNT          518 a          617 c          637 g          527 t
ORIGIN      
        1 gttcctagag accacatgtt tgcgtttccc gaagacgtcg gtgggcgttt ttccgtgttg
       61 agcgccgtgg ggctcgtgcc cgccgcaatt ctgggactga acatcgagga gcttctcgaa
      121 ggggcatgcg ctgccaatcg tgcgttttcc aacagtgatc cggcgagcaa tcccattttg
      181 cgatatgtgg gcgtctgcca cctcatggaa agccgccgtg gggcttccat acgtgtgtta
      241 tctacctggg gaaagcggtt ggaagccgtg ggactgtggt acgaccaact cctttcagaa
      301 agtctgggaa agaacgggca gggggctacg ccgttgacag ttgtcaatac acgcgatctt
      361 cactcccgcg gacagcagca tcaggaaggc cggcgggaca aactttttac aaatctctgg
      421 gctgagcgac ctagtctagc ttgtgttacg attccccgat gggattacga cgatgatcag
      481 cttaataaac tcgcgggaaa gtcgctttcg gatattcttc gcgcggcccg actgggaacg
      541 acccaagcct atgccgagga tttgcgtcgc tccgccgaga tcattcttcc gcaaattcgg
      601 gaacgcacgc tgggtgaact ctttcagttt ctgatgttgg caactgttgt cgagggccga
      661 ttaattggaa tcaaccctta cggtcaaccc ggagttgagg cttacaagcg acacatgcag
      721 aaactcttag agcagtagcc aaggacaatt ccgattccca aaggggaggg gggccaccgc
      781 ggactgaaaa accggtacta ccaatgcaca atggcgttgg gtcatcggcg tttcctgaga
      841 gaagggagcc aagaacatgg caacgcagct acgctggcta ggacacggtt gttggctaat
      901 cgaaaccaat ggccatcgta tcatccttga cccctttctg gacgagtcgc ctgtggcgcc
      961 gattaaagct gatgaagtaa aggcagacta tgtcttgatc tctcacggtc actttgacca
     1021 tatggccgat gcagagtcga tcgcaaaagc caacgcggcc acggtgattg ccacttacga
     1081 aatttgcgag tggctaaacc gaagaggggt cacatcgaca cacgccatga atgtcggggg
     1141 gacgcatgca ttcccctttg gccgagtcaa gatgacaccg gcggtgcata cttcaatgct
     1201 tcctgacgga agttacggtg gcgtggctgt gggattcctc ctacttcttc aagatggtaa
     1261 cgtctatttt gcctgtgata ccggtctttt cctggacatg cagttgatcg gcgatcacga
     1321 cttggccctg gccgtcctgc ctatcggaga taacttcaca atgggtccag aggatgcctt
     1381 gcgggcagtc acactgttgc gtcccaaacg ggtcgtacct gtccacttta acacatggcc
     1441 tttgatcgcc caggatgtca atgattgggc caatcgcgtg agacacacca ccgcatcagt
     1501 gcccgtcgtc ctgcagccgg gtgagagcat cgcgcttggc taaggtgtca agtagcttcc
     1561 tcgcgcgata agctcgttaa ccggtggcgg tgaagggtat caggtgatgg tctcgacgaa
     1621 gtgtcaaggc gttattccac tggctttttg agcagaaccc agccgtcgcg gacggtaacc
     1681 ttgtaggttg gaacagacaa cgttgggaca tccgtgcacc gaccatcggc aagacgaaag
     1741 gcccacatgt gccgcggaca aacgacggtc ccttcccaaa tccgcccggc actgagcgaa
     1801 tcacccatat gggggcaata atcatccgtt gcaaaaacgc cattctcggt gcgaaagata
     1861 gcaatctcgc gtcctgcgac gacaaatcgc tttcccttgt ttttttgtag gtcatcgagc
     1921 ttacagaccg gaatccactc gccactcgat tgccaatcaa aaaggtccat tccgcactcc
     1981 ttcgtctttg tggtcacacg cacccaaacg ggcccacagg aaaaataccc acgatgtgat
     2041 gcccaggtgc catcctttcg ggtctatcct cgtggtattc tatcggtatt cacaaggtcc
     2101 actggactga ttccggttca ggcgattccg cctgttgggt cgccgaaatg ggatgcaggt
     2161 tcgttatctc gttgcttgga acgaccatta ccgggattca tctcaacaga ggtttagagc
     2221 tgtgaagaag aatgccgtaa aattggcctt gcgggagagt cccaccgttt ggggaacctg
     2281 gggtaccatc cccgatgac
//
LOCUS       DAOPTR010000677         2294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_37081_length_2294_cov_3.791871, whole genome shotgun sequence.
ACCESSION   DAOPTR010000677 DAOPTR010000000
VERSION     DAOPTR010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37081_length_2294_cov_3.791871"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="PKI05_12035"
     CDS             complement(<1..772)
                     /locus_tag="PKI05_12035"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007338657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HOA52976.1"
                     /translation="MATEFRLAFERPIYELESRIEKLERLAQTDPNVREELQQKRLEL
                     VELKKQIYSNLKPWEVVEVARHPDRPKTSDYLQLTFDEFVELHGDRCFGDDRAIRTGW
                     AKLDMFKVMVIGHEKGKTTKERLACNYGCAHPEGYRKAMAKAKLAAKFHLPIICLIDT
                     PGAYPGIGAEERGQAQVIAESMFLMSQLPTPIICVVIGEGGSGGALAIGVGDRVAMLE
                     HAYYSVISPEGCAGILWKDRQYKEEAARALKMTSKDLIQ"
     gene            complement(834..1712)
                     /locus_tag="PKI05_12040"
     CDS             complement(834..1712)
                     /locus_tag="PKI05_12040"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOA52977.1"
                     /translation="MSTAAVTYIGRYRLVNPVYLGATTQLWQAFCDSTGQFVALKLLL
                     EKFRRDREQIRSLKWEYTIASQFDDPRIIKIFEFNIDRGIPYLAMEWFNGGNMKNLIR
                     RGIEEYAPFVRKIIFQCIEAVSYLHRQGFVHRDIKPENFLVSPQGDVKLIDFALCRKI
                     GPKILWLFRPKSKIQGTCSYMSPEQILNKPLDGRADLYSLACTIFELVCGKPPFTGMN
                     QTELLNKHLKSNPPPLEAYNPNVHPEFSRIIQKALAKRPSDRFKSIGEFAGALYDVPV
                     FKVPPPDEVPVQLTLA"
     gene            complement(1835..>2294)
                     /locus_tag="PKI05_12045"
     CDS             complement(1835..>2294)
                     /locus_tag="PKI05_12045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007338660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA52978.1"
                     /translation="LWVGEGILRPRLEAQIRSRGWEGRFHLAGLVPPDDLPRWLSAMD
                     VIVHTSLREGLARVLPQALLSGKPVVSYDIDGAREVVIPGQTGYLIPPRNLSGLIAAL
                     EELVQTEHLRQTLGKRGQELCSQEFDRYKMTERIRALYVRLLKEQGKAQG"
BASE COUNT          512 a          630 c          596 g          556 t
ORIGIN      
        1 gctgaatgag atctttggag gtcatcttta gggcgcgggc agcttcctct ttgtattggc
       61 ggtccttcca gaggatgccg gcgcagccct cgggactgat caccgagtaa tacgcgtgtt
      121 ccaacatggc cacacggtca cccacgccaa tggccaaggc accgccggaa cccccttcac
      181 cgatcaccac gcaaatgatg ggcgtgggta actgggacat gaggaacatc gattcggcaa
      241 tcacttgcgc ctgcccgcgt tcctctgcgc cgatgccggg ataagcccct ggcgtgtcga
      301 tcagacaaat gatcgggagg tgaaacttgg cggcgagctt ggccttggcc attgccttgc
      361 gatatccttc cggatgagcg cagccgtagt tgcaagccag ccgctctttg gttgttttgc
      421 ctttttcatg gccgatgacc atgaccttga acatgtcgag cttggcccaa ccggtccgta
      481 ttgcccggtc atccccaaaa caacgatccc cgtgaagttc cacgaactcg tcgaatgtca
      541 attgcagata atcgctggtc ttcgggcggt ctgggtgccg agcgacttcc acgacctccc
      601 aaggctttaa gttactgtag atttgcttct ttagctcgac gagctcgaga cgcttttgct
      661 gcaactcctc gcgaacgttg ggatctgtct gagccaatcg ctcgagtttt tcaatgcggc
      721 tttcaagctc gtaaatggga cgctcaaatg cgaggcgaaa ttcggttgcc atggcttcca
      781 cttcctgtct tagtcataag atgggtggcg tatcgctttt agtagttaca cagctacgcc
      841 agcgttaact ggaccgggac ttcatcgggc ggcggtactt tgaaaacagg cacatcgtac
      901 aatgctccgg cgaactcgcc aatcgacttg aaacgatcgg aggggcgttt ggccagggct
      961 ttctggatga tgcgggagaa ctcgggatgc acgttcgggt tatacgcttc taacggaggc
     1021 gggttagact tcaggtgctt attcaggagt tcggtttgat tcatcccggt gaatggtggc
     1081 ttgccacaga ccaattcaaa aatcgtacag gctaggctgt agagatcggc cctcccgtct
     1141 aagggcttat ttagaatctg ctcgggggac atataactgc aagttccttg gattttcgat
     1201 ttggggcgga acaaccaaag aatcttcggc cctatttttc ggcacaaggc aaagtcgatc
     1261 agcttaacat ccccctgtgg agaaacaagg aaattctctg gtttgatatc gcggtggaca
     1321 aaaccctggc gatggaggta cgagacggcc tctatgcatt gaaagatgat cttccgcacg
     1381 aacggggcgt actcctcgat ccctctgcgg atgagattct tcatattgcc gccattgaac
     1441 cactccatgg ccagataggg tattccgcga tcgatgttga actcgaagat cttaatgatg
     1501 cgtggatcat cgaactggct tgcgatcgta tattcccatt tcaggctacg gatttgttcg
     1561 cggtcgcggc gaaatttttc tagcaataat ttgagcgcaa caaattgacc agttgaatcg
     1621 cagaatgcct gccacaactg tgtcgttgcg cccaaataca ccggattgac cagtcgatat
     1681 cgcccaatat atgtaactgc cgcagtactc acgctcgcac gactcgcttc aattagttca
     1741 tatcacccag tttgctcatt ttgcccacaa cctatttccc gtacgatcct tgtgatcatc
     1801 ttaacgatgt ggaccagcaa actcaaccac aaatttaccc ctgggcctta ccctgctcct
     1861 tcaacaagcg gacgtacaac gcacggattc tttcagtcat cttgtagcga tcaaactctt
     1921 gcgagcaaag ctcttggccc ctcttgccaa gtgtctgcct taaatgctcg gtttggacga
     1981 gctcctcgag ggccgctatg agtcctgaca ggttacgcgg aggaataaga tagcccgttt
     2041 ggcccggtat gacgacttcg cgggcaccgt cgatatcgta actcaccacg ggctttccgg
     2101 agaggagcgc ttgcggcaat acccttgcca ggccttcacg aaggctggta tggacaatga
     2161 cgtccatcgc cgagagccag cgaggcaggt catcgggcgg aacgagacct gccaaatgaa
     2221 accttccttc ccaaccgcgg ctgcgaatct gtgcctctaa acgtgggcga agaatcccct
     2281 cccccaccca gaga
//
LOCUS       DAOPTR010000678         2282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_37350_length_2282_cov_3.736866, whole genome shotgun sequence.
ACCESSION   DAOPTR010000678 DAOPTR010000000
VERSION     DAOPTR010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37350_length_2282_cov_3.736866"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..>2282
                     /locus_tag="PKI05_12050"
     CDS             89..>2282
                     /locus_tag="PKI05_12050"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOA52979.1"
                     /translation="MAPPPAPQPAAPRPEQVAGPGQTPSADQQIPQLPSADVKLRFNF
                     RYQPWKEVLDWLARQAGLSLVMEAPPPGTFNYVDDHEFTPAQAIDLLNSVLLTKGYVL
                     VRRERMLILFNVDDGIPPNLVDTVSPEDLDNRGDYEMVSCLFQLRRMTPEEAEQEVKK
                     LLGPQGSVVVLAKAKQLFVTETAGRLKTIRRMLDAIENPSANDMVRTFRINPSTVDQV
                     LSIIRQMFQIPADQTANADGTLRFAIDPSASRLLVSGSPEMLDRFSQLLQALDPSATD
                     GGSALESTPQLEVYPITSADPQMVLQVLQTLLAGLPDVRLAIDPKTGALVAWARPAQH
                     ATIRATLQQLQGEVGQVEVIPLRVVDPQLAVLAISKLFGTGDSASGGAPTVDADLSTR
                     MLLVKGTKGQIEQIRQLLIKMGEPADTSGSANLSLMRTIPMDAATAQAVLDQMQNLWP
                     QVRPNRLQVIRSVVPADAGRPSPAANDVEQSQTGNEQTNGGIESQRIPLPGVMTSSSR
                     LVLGLSPQRSTSSTSTTSSSTSSPAPQQPSFPQPAPGQPPFFRPWGPPGGFSPGGPGG
                     FFERRSDGGDRGYDRWRGSDRTTYAHGDNIQLAESGDGLGVMSSSGDSPTGRPWAPVV
                     FVSQESKVVNEGNSAAPPDVQDSSSQQGSTAQTSSEPSPEASAPSETGEKRTTEFPAP
                     LEGPPSAPPVIVAITPEGLMVASQDPEALNQFENLVRRMVSQDVTQYGG"
BASE COUNT          501 a          668 c          670 g          443 t
ORIGIN      
        1 cggggcacca agtcctggag aagtcaaaga gagattcggg gagcgacctg gccctctacc
       61 tcccggaccg cccggcgcta tccccggtat ggcaccaccc ccagctcccc aaccggcggc
      121 accacgaccg gagcaggtcg ccggacctgg tcaaacgcca agtgctgacc agcagattcc
      181 gcaacttccg tcagcggacg tgaaattgcg ttttaacttt cgctaccaac cgtggaaaga
      241 agtgcttgat tggcttgcac gccaggcagg cttatcgctt gtcatggagg ctccgccgcc
      301 ggggacattc aactatgtcg atgaccacga gttcacgcct gcgcaggcta ttgatcttct
      361 caatagtgtc cttttgacaa agggctatgt ccttgtgcgg cgagaacgaa tgctgattct
      421 tttcaacgtg gacgacggga ttcccccgaa tttggtcgat acggtctccc cggaagactt
      481 agataacagg ggggattacg aaatggttag ctgtctcttc cagctccgcc gaatgacacc
      541 cgaggaagca gagcaggagg tgaaaaagct cttgggaccg caagggagcg tcgtcgtctt
      601 agcgaaagcg aagcagctat tcgtcacgga aacggcaggg cggctcaaaa ccattcgaag
      661 aatgctggac gcgattgaaa atccctcagc taatgacatg gttcggacct tccggatcaa
      721 tccttcgacg gttgaccagg tgctcagtat tatccgccag atgttccaga ttccggcgga
      781 ccagacggct aacgctgacg gcacgctgcg ttttgctatt gatcctagcg ctagtaggct
      841 tttggtatcc gggtcgcctg agatgctcga tcgcttcagc caactgctcc aggcacttga
      901 tccctcggca accgatggcg ggtcagcact agagagcacg ccacagttgg aagtgtatcc
      961 catcacatcg gccgacccgc agatggtctt gcaggtattg caaacgttgt tggcgggctt
     1021 gcccgacgta cggctggcga ttgacccaaa gacgggggcc ctggtggcat gggcaaggcc
     1081 agcccagcat gcgacgattc gagctacgtt gcaacagtta cagggtgaag ttggccaagt
     1141 ggaggttatt ccgctccgcg tcgtcgatcc acaattggct gtccttgcca ttagcaagtt
     1201 gtttggcaca ggggacagcg cttccggcgg tgccccaacg gtggacgctg atttgtcgac
     1261 acgaatgtta cttgtcaagg ggacaaaggg gcagatcgaa cagattcgtc agcttctgat
     1321 caagatgggg gaacccgctg acacctccgg cagtgctaat ctcagcctca tgcggaccat
     1381 ccccatggac gcggcaaccg cgcaagccgt gctcgaccag atgcaaaacc tctggccgca
     1441 agtgagaccc aaccggttgc aggtgatcag atctgtggta ccggctgacg caggccgacc
     1501 atcaccggcg gcgaacgacg tggaacaatc gcagactggc aacgagcaaa cgaacggggg
     1561 aattgaatcg caacgaattc ccttgcctgg tgtcatgacc tccagcagcc gcctggtcct
     1621 ggggctaagc ccacaacgtt caacgtcttc aacaagtacg acgagctctt cgacgtcctc
     1681 acccgcacca cagcagcctt cattcccgca gccagcacct gggcaaccgc catttttcag
     1741 accctgggga ccacccggtg ggttttcgcc cggagggccg ggcgggttct ttgagcggcg
     1801 gtccgacggg ggtgaccgcg gttacgaccg ctggcgggga agtgacagga ctacgtatgc
     1861 ccacggcgat aacatccagt tagcagagtc tggtgacggc cttggtgtca tgtcttcctc
     1921 tggtgactcg cctaccgggc gaccgtgggc acctgttgtt ttcgtctctc aggagtcaaa
     1981 agtcgtgaat gagggaaatt cggctgcgcc gccggatgtg caggatagca gctcacaaca
     2041 gggcagtact gcacaaacct cctcggagcc cagccccgaa gccagcgcac ccagcgaaac
     2101 cggggaaaag cgtacgacag agtttcccgc acccctcgaa ggcccgccga gcgcaccacc
     2161 cgtcattgtg gccatcacgc cggaaggact gatggtggca agtcaggacc cggaggcgct
     2221 taatcagttc gaaaacctcg ttcggcggat ggttagccaa gatgtgactc aatatggtgg
     2281 tc
//
LOCUS       DAOPTR010000679         2275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_37491_length_2275_cov_3.290991, whole genome shotgun sequence.
ACCESSION   DAOPTR010000679 DAOPTR010000000
VERSION     DAOPTR010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37491_length_2275_cov_3.290991"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PKI05_12055"
     CDS             <1..458
                     /locus_tag="PKI05_12055"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOA52980.1"
                     /translation="KVVLVDFWATWCPPCMELLPHTLELAKKYASRGVTVILVAINDP
                     DEQADDVKSVLERNQARGVVNLISRYGIGLEAMEKFEIDDGALPHLRLYDRQGRLWAK
                     FGVSAIAPDPKRIEAEVEHLLAAPEEAASHEPTAGPELVIPEGPSSSTS"
     gene            488..1291
                     /gene="panB"
                     /locus_tag="PKI05_12060"
     CDS             488..1291
                     /gene="panB"
                     /locus_tag="PKI05_12060"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOA52981.1"
                     /translation="MSAESAPLTVPKFRALKRSGRKIVMVTAYDYPSAALADLVGVDG
                     ILVGDSLGMVVQGKATTLSVTLRQMVYHAEMVARATRHALVVVDLPFLSYQTGVRDAL
                     KNAGRILKETLAQAVKLEGGREQAELIAALTGAGIPVLAHLGLRPQAIRASGGYRVER
                     DRDRLLADAEAVQQAGAFAVILECIPASVAEEITGRVDIPTIGIGAGPGCDGQILVFH
                     DLLGYSTQPRRPKHAKQYADLGQIIQEALRRYIEEVQTGQFPGPEHSFS"
     gene            complement(1467..2072)
                     /locus_tag="PKI05_12065"
     CDS             complement(1467..2072)
                     /locus_tag="PKI05_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HOA52982.1"
                     /translation="MIDVRAITELHQETVQRWHHQGIDNPYTGFLALVCEQHQRNYLL
                     WHEEDKARDPQATDVQIAAVKRRIDRLNQERNDWIERLDEAIMKALAAWGVILRPRAR
                     LNTETPGSAIDRLSILALRIYHMEEQTRRSDVDEAHRAKAREKLEILRRQHADLSRAL
                     AELLADIFAGRCEMKIYRQFKMYNDPAYNPYLYQTGQRRAA"
BASE COUNT          438 a          640 c          695 g          502 t
ORIGIN      
        1 gcaaggtggt ccttgtggac ttttgggcaa catggtgtcc gccgtgtatg gagctgttac
       61 ctcatacgtt ggaattggcc aaaaaatacg catcacgggg cgtgaccgtg atccttgtgg
      121 caatcaacga tccagatgag caggccgatg atgtcaagtc cgtgttggaa aggaatcaag
      181 cgaggggcgt tgtgaacctg attagccgct acgggatcgg tttggaagcc atggaaaaat
      241 ttgagatcga cgacggcgca ctgccgcatc ttcggttata tgaccgccag gggcgattgt
      301 gggccaaatt cggtgtttcg gccattgcgc ccgacccgaa gcggatcgaa gccgaagttg
      361 aacatctgct ggccgcaccg gaagaggcgg catctcacga gccgacggcc gggcccgagt
      421 tggtcattcc cgaaggccct tcatcgtcca cgtcctgagg tgttcgccct tggttgagga
      481 ctgtcccatg tctgccgagt cggccccatt gacggttccc aagttccgtg ccctgaagcg
      541 atcggggcga aaaatcgtga tggtcacggc ctatgactat ccttcggcgg ccctggcgga
      601 cttggttggc gtggatggga ttctcgtggg agatagcctg ggcatggtgg tccaaggaaa
      661 ggccacgacg ctctcggtaa cgctgcggca gatggtgtat cacgcagaga tggtggcccg
      721 ggcgactcgc cacgccttgg tggtggtgga ccttcccttt ctcagttatc aaacaggcgt
      781 ccgggatgcc ctcaaaaatg ctggacggat tctcaaagag acactggccc aggccgtgaa
      841 gctagaaggt ggccgggaac aggccgaact cattgcagcg ttgaccgggg cagggattcc
      901 agtgttggcc catttgggac tgcgtccgca agcgattcgg gcctcgggcg gctatcgtgt
      961 ggaacgcgat cgcgaccggc tccttgccga tgccgaggcc gtgcagcagg ccggggcgtt
     1021 tgccgtcatc ttggaatgta ttccggcctc tgtggcggaa gaaatcactg gccgcgtgga
     1081 cattcccaca atcgggatcg gcgcaggacc tggttgcgac ggccagattc tcgtctttca
     1141 cgacctgctg ggctacagca cacagcctcg tcgccccaag cacgccaaac agtatgccga
     1201 tcttgggcag attattcagg aagctttgag gcgttatatc gaggaagtcc aaacggggca
     1261 gttccccggt ccggaacaca gcttttcctg atctcggaca gcgtgcctaa aagcctatcc
     1321 cacaacctcc cctctcccgt tcggcgggag aggggccggg ggggagggcc ggctcgaatc
     1381 gctgctggtt ctctcgatcg actccgacga tggctcgccc tcaccctaac cctctcccag
     1441 agggagaggg aattttggga taggctttaa gccgcccgtc gctgcccagt ctggtaaaga
     1501 tagggattgt aggcaggatc gttgtacatc ttgaactgcc ggtaaatctt catttcgcac
     1561 cgcccggcaa agatgtcggc cagtagttcc gctaacgcgc ggctaagatc ggcgtgttgc
     1621 cggcggagga tctcgagttt ctctcgggct ttagcacggt gcgcttcgtc cacatcggac
     1681 cggcgagttt gctcctccat gtggtaaatc cgcaaggcca aaatggaaag ccggtcgatc
     1741 gcactaccag gcgtctcggt gttgagtcgt gcccgtggcc gcaagatcac gccccatgcg
     1801 gcgagggcct tcatgatggc ctcatcgagc cgttcgatcc agtcgtttct ttcttggttg
     1861 agtcgatcaa tacggcgctt taccgcggcg atttggacat cggtggcctg cgggtcgcgg
     1921 gctttgtcct cttcgtgcca gagaagatag ttgcgttgat gttgttcaca aaccaacgca
     1981 aggaaccccg tgtaaggatt atctatgccc tggtgatgcc agcgctggac ggtttcctgg
     2041 tgcagttccg tgatggcccg cacgtcaatc atggcttcct tccttggaca tttcacacgt
     2101 cctggggacg actcacgaat gatcaaacct cgattcgtct cggcaggatg gtgcatcacc
     2161 caacctccat gtcagggata cccaagggca ggagttacca cacctcgttt tcccgccttg
     2221 gggcttcgag actatgaact ccctgccgcc tttcatgggg caattctcga tcacg
//
LOCUS       DAOPTR010000680         2275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_37494_length_2275_cov_2.973423, whole genome shotgun sequence.
ACCESSION   DAOPTR010000680 DAOPTR010000000
VERSION     DAOPTR010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37494_length_2275_cov_2.973423"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..634)
                     /gene="rplO"
                     /locus_tag="PKI05_12070"
     CDS             complement(140..634)
                     /gene="rplO"
                     /locus_tag="PKI05_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOA52983.1"
                     /translation="MNIDQVNRGVVTHKKRKRVGRGPGSGHGKTCGRGHKGARSRSGW
                     REKAGFEGGQMPLIRRVPKRGFNNARFALRIAEINLEVLEKAFADGDEVTPEILEQRG
                     LVKRVYDELKILGNGELTKKLSVSAHRFSRSAKEKIEKAGGKVIVLPGKTPVEEKKKA
                     VKGA"
     gene            complement(631..1122)
                     /gene="rpsE"
                     /locus_tag="PKI05_12075"
     CDS             complement(631..1122)
                     /gene="rpsE"
                     /locus_tag="PKI05_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOA52984.1"
                     /translation="MASDLEPIRGQLIEKVLSVRRCAAVVKGGRRFSFTALVVVGDGM
                     GKVGWGYGKANEVPPAVAKAIKDGMRRMKTVTIVDGTIPHQVKGSFGAAEVVLVPAGP
                     GTGVIAGAAVRAVCEAAGIKNILTKSYGNTNPINLVKATMNALEQLRSKAEVERLRGT
                     SLS"
     gene            complement(1269..1631)
                     /gene="rplR"
                     /locus_tag="PKI05_12080"
                     /pseudo
     CDS             complement(1269..1631)
                     /gene="rplR"
                     /locus_tag="PKI05_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008666786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
     gene            complement(1754..>2275)
                     /gene="rplF"
                     /locus_tag="PKI05_12085"
     CDS             complement(1754..>2275)
                     /gene="rplF"
                     /locus_tag="PKI05_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOA52985.1"
                     /translation="VPIPQGVTVTIEGRVIRVKGPKGELTFEHRPEVSVSVDTARSAV
                     VVERVQETRLARALHGLTRALVNNMVIGVTQGYEKRLEISGVGYLAAVQKGVLQLRVG
                     YANELQVPIPEGLEVTCPDQQHIVIRGVDKQKVGNFAAAVRALRKPDPYKGKGIRYQG
                     EHIRMKEGKVSAK"
BASE COUNT          421 a          748 c          562 g          544 t
ORIGIN      
        1 atccgataga tcgccaagag cagtagggtg agcaggattt tctgtctcag ttcccgaatc
       61 gtaaagataa cacgaatctt ttcccacata ggccggtccg tccatcacag cgccgaggtc
      121 actcgtgacg cgtttacgct tatgctcctt tgaccgcctt cttcttttct tctacgggcg
      181 tcttgccggg caagacgata accttgccgc ctgccttttc gatcttttcc ttcgcggaac
      241 ggctaaaacg gtgcgccgaa acggagagtt tcttggtcaa ttcgccatta cccaggattt
      301 tcaactcgtc gtaaacccgt ttgacaaggc cgcgctgttc gagaatttcg ggcgtaacct
      361 catcgccatc tgcgaaggcc ttctccagca cctcgagatt gatctccgca attcgcaggg
      421 caaaacgggc attgttgaag ccgcgcttcg gaacccggcg aatgagcggc atctgaccgc
      481 cttcgaagcc tgccttttct cgccagccac tccgcgagcg ggcaccctta tgtccccgcc
      541 cgcacgtctt cccgtgcccg gaacccgggc cacggccaac tcgcttgcgt ttcttgtggg
      601 tgacgactcc tcgattgacc tggtcgatat tcatgacaac gatgtccctc tcaagcgttc
      661 cacttcagcc ttggaacgga gttgctccag ggcgttcatt gttgctttca cgagattaat
      721 agggttggtg tttccgtaac ttttcgtcag gatgttcttg atcccggcgg cttcgcacac
      781 cgcccgaacc gcggcgcccg caatcacacc ggttccgggt ccagccggga ccaggacgac
      841 ctcagcggcc ccgaacgaac ctttgacctg gtgcggaatc gtgccatcaa caatcgtcac
      901 ggtcttcatt ctccgcatgc catccttgat ggccttcgcc acggcaggag gcacttcgtt
      961 ggctttccca tagccccagc cgaccttgcc catcccatca ccaacaacga cgagggccgt
     1021 aaagctgaac cggcggcctc ccttcacgac ggcggcgcac cgacggacac tgaggacctt
     1081 ctcgatcaat tgaccacgaa tgggttccaa atctgacgcc atgtttcaac ctttacaggg
     1141 aatgttctcg ttgttgatca acccttcttt ccagctttct cgccttttcc agaggttgcc
     1201 ttctcgccct tcttcttcgg tttttcctcg gcgggctccg cttccggtgc tttcttcggg
     1261 ccaatatcca ggccggcgtc tcgtgcggca tcggctagag cggccacccg accatgatac
     1321 ttatacggcc cgcggtcgaa aacgaccttc gtgatccccg ccgcaagggc acgttccgct
     1381 atcacttttc ccaccacggc agcggccttc acattgccac cgtagccacc gaccatctcg
     1441 cggatcgccg gctccaccgt acttgccgcg gccagcgtcc gtccttggct gtcgtcgatg
     1501 acttgggcat aaatatgctt gttgctgcgg aacacgctga gtcgcggtcg gttcgagacc
     1561 ctgcggagct tgttccgcac tcgaaagact cgccgcaatc gacgtctttg caatcgcttg
     1621 agctgactca cgactggcct ctctttttga cttgcactct cgctcgttat ttcctgttgt
     1681 tctaccgcac ccgctgggcg actcccgaaa caatcctcac gcgttcatgc gacacgtcat
     1741 cgtttcccgg ggactacttg gcggacactt ttccttcctt catacggatg tgctcgccct
     1801 ggtaacggat gcctttaccc ttgtacggat cgggcttacg aagcgccctg accgccgcgg
     1861 cgaaattgcc aaccttttgt ttgtccacac cgcgaatcac gatgtgttgc tgatccggac
     1921 aggtcacctc aagtccctca ggaatgggaa cttgaagctc gttggcataa cccacgcgga
     1981 gttgcaatac ccctttctgc accgcagcca gataacccac gccggaaatc tccaacctct
     2041 tttcgtagcc ctgggtgact ccaatcacca tattgttaac gagggcccgt gttagtccgt
     2101 gcagggcccg ggccagccgg gtctcttgaa cacgttcgac aacgacggcc gaacgtgccg
     2161 tatcaactga cacggacacc tccgggcgat gctcgaacgt taactcaccc ttggggcctt
     2221 tgacacgaat tacccgaccc tcgatcgtga cggtcacgcc ttgtggaatc ggaac
//
LOCUS       DAOPTR010000681         2266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_37677_length_2266_cov_2.274989, whole genome shotgun sequence.
ACCESSION   DAOPTR010000681 DAOPTR010000000
VERSION     DAOPTR010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37677_length_2266_cov_2.274989"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PKI05_12090"
     CDS             <1..356
                     /locus_tag="PKI05_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52986.1"
                     /translation="YHEPNAFAYKLRRLRLEHFECDNNILWHQGGSVLISLDDKPRTF
                     QEWQQLGFDLHSLVADPKFVDPAKDDYRLQPDSPAFQLSFQPIPVEKIGPYADSLRAS
                     WPIQEAPGVREMSYK"
     gene            643..729
                     /locus_tag="PKI05_12095"
     tRNA            643..729
                     /locus_tag="PKI05_12095"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:677..679,aa:Ser,seq:tga)
     gene            complement(1114..1695)
                     /locus_tag="PKI05_12100"
     CDS             complement(1114..1695)
                     /locus_tag="PKI05_12100"
                     /inference="COORDINATES: protein motif:HMM:NF025696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOA52987.1"
                     /translation="MIENNPSNVSAAFEMLLEEIEAEVDFVNRIGARAFEGREYDKAR
                     EALERVAALTAFRDKVAALRKEWDAMAAVADRAEDEETRSQRRNMGRLPKGVRTPEKA
                     FYRPILQALQEFGGTAEIGKVLEKVEELMKPILKDVDYEPLASDADLPRWRNTAQWAR
                     NSMVKEGLLKADSPRGIWAISDVGRKYLAESGG"
     gene            complement(1710..>2266)
                     /locus_tag="PKI05_12105"
     CDS             complement(1710..>2266)
                     /locus_tag="PKI05_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52988.1"
                     /translation="LRGTNALEYIKWGESEGFHKRPSCRGRQRWWDLGDRRAPPIVSP
                     SSISGFYRAFLNDARVLIDKRLYEVIPNAGGARELAVSLNCTLTTLFLELGSRIGLGE
                     GLLDMTVYEVANCLTLGPFLVKDVPLFNRPVQEVECEIRQPDRRALDDIVFDVLGLTA
                     GEREAAYEAVVNLVRARLEKARSV"
BASE COUNT          467 a          686 c          585 g          528 t
ORIGIN      
        1 actaccacga gccgaatgcc tttgcgtaca aattgcggcg actacgactg gagcattttg
       61 aatgcgacaa taatatcctg tggcatcaag gcgggtcagt gttgatcagt ctagatgata
      121 agcctcgaac ctttcaggaa tggcaacaac ttggtttcga ccttcactcg ctggttgccg
      181 atcctaagtt tgtcgatccc gccaaagatg attatcgact ccagcccgac tcaccagcct
      241 ttcaactgag ctttcagcct atcccggtcg aaaagatcgg tccctatgcc gactccctcc
      301 gggcaagctg gccaatacag gaggcccccg gcgtgaggga aatgtcatac aaatgagctg
      361 ccccaaaata agttggtcgt ccttccggct gctctgctga ttgtgtcgag taaccagact
      421 tttcataaag tgatccagcc ggcacgtccc cttgggacct ttacggagtc ggttcagact
      481 ggtaaagtag gttacgttag ccgggttcga tctcattgaa taagacgaat accgcgcagc
      541 cgagaactac cgcatcgcac acgcaggcgg ggtttagctt gagtgtgtcg cgttcgatgt
      601 gggtgttgtt ttattacacc cacccgagtg gaattgctag gtggatgggt ggccgagtgg
      661 tctaaggcgg cagtcttgaa aactgctgta cccgttcggg taccgggggt tcgaatccct
      721 ccccatccgt ttggcggggc atgcccctgt cctttcgatg accaaacggt aggggcaacc
      781 ccccgtggtt gccctgattg tgcccccgtg gttgcccgga ttatgataat tgccgagccg
      841 gccccgaatc tggtgttatt cttgcgttaa cgcaccatgt cggctggtaa ccgccgctgt
      901 ccgccagata cttccgctga ccaggaaggt taggagctat tccaaaaact cccctctccc
      961 tctgggagag ggttggggtg agggcaagca aacggcggaa tggattgaga aaacctggcg
     1021 cgattcgagc caccctcact cccgacccgt ctcccgccga tcgcgcgagg ggaggtcgtg
     1081 ggataggctc tcagcttctc aagacccaag tcgttaaccg ccactttccg cgaggtactt
     1141 ccgccctacg tccgagatgg cccagattcc ccgcggcgaa tcggctttga gaagcccttc
     1201 tttgaccata ctgttgcgtg cccattgggc ggtgttccgc caccgaggca ggtcggcatc
     1261 acttgcgaga ggctcgtagt ccacatcctt caaaataggt ttcataagct cttccacttt
     1321 ttccaatacc tttccgatct cagcggtgcc gccgaactct tgcaacgcct ggaggatggg
     1381 acgataaaag gccttttcgg gcgtgcggac gcccttgggc aggcggccca tattgcggcg
     1441 ctgcgaacgg gtttcctcgt cctcggcgcg gtcggctacc gcagccatcg catcccattc
     1501 cttgcgcaat gctgcaacct tgtcgcggaa ggcggtcaac gccgcgaccc gctccaaagc
     1561 ctcgcgggcc ttgtcgtact cgcgcccttc gaaggcacgt gcgccaatac ggtttacgaa
     1621 atcaacttcc gcttcgattt cctccaaaag catctcgaag gccgctgaca cattgctcgg
     1681 attgttttca atcatggctt gctcctttct cacacacttc tggccttttc caggcgggcg
     1741 cggacgaggt tgaccaccgc ctcatacgcc gcctcccgct ctcccgccgt cagccccaac
     1801 acgtcaaaaa cgatgtcatc taaggcccgt cggtcgggtt gacgaatctc acattcgacc
     1861 tcttgcacgg gtctattaaa taaaggcacg tcctttacga gaaatggccc tagtgttaaa
     1921 caattcgcaa cctcgtaaac cgtcatgtcc aatagcccct cgcccaagcc tattcgagaa
     1981 cccagctcaa gaaacaaggt cgttaaggtg cagttcaagc ttacggcaag ttccctagcg
     2041 cctccggcgt ttggaatgac ctcgtacagg cgtttgtcta taagcacccg ggcatcattc
     2101 agaaacgcgc gataaaaacc gctgatgcta ctcggagata caattggcgg cgctctccta
     2161 tcccctaaat cccaccacct ctgccttcct ctgcagcttg gtcgcttatg aaatccctct
     2221 gattcgcccc acttgatgta ttcgagggcg tttgtgccgc gcagct
//
LOCUS       DAOPTR010000682         2264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_37713_length_2264_cov_2.747397, whole genome shotgun sequence.
ACCESSION   DAOPTR010000682 DAOPTR010000000
VERSION     DAOPTR010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37713_length_2264_cov_2.747397"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PKI05_12110"
     CDS             <1..360
                     /locus_tag="PKI05_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52989.1"
                     /translation="ASFVVLPIVVIDVIRVSNKFVGPLLRLRQSVRSLAAGQDVEPLR
                     FRKGDFWRSFADDFNALVSRIKHLETAAHQSKGTSADPWQEDRPLAESGATPGPTPVG
                     DILAVGGNLDGPVMHRV"
     gene            567..1037
                     /locus_tag="PKI05_12115"
     CDS             567..1037
                     /locus_tag="PKI05_12115"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOA52990.1"
                     /translation="MMSQTGEVSKTSIKEILLAKGSHVYTIDPDATLQDVVEELVQHN
                     IGALVVCHRDPDRGEKILGIITERDILRRRAYSTGPLSEVRVSEVMSRNLITAQPDDT
                     VAHALQLMTIHRVRHLPIVLDGQLVGIISIGDAVKAQLEHLMLENRFMKDYIQG"
     gene            complement(1052..>2264)
                     /locus_tag="PKI05_12120"
     CDS             complement(1052..>2264)
                     /locus_tag="PKI05_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOA52991.1"
                     /translation="ESKVPAYTLPDPLVCEDGTVVRDAATWMQKRRPELLQLFQTHVY
                     GRSPGRPADLSFVLKSRDDNALGGKAVRKEVRIHFSAKKDEGPGMDLLIYLPKEAPRP
                     VPVFLGLNFRGNHAVHPDPAISIPTSWMRPGEGVVNNRATEAGRGTAASRWPIEMVLE
                     HGYGVATAYYGDIDPDFDDGFQNGVHPLFYKEGQTRPAPDEWGSIGAWAWGLSRAMDY
                     FETDDDIDEKRVAVLGHSRLGKTALWAGAQDERFAIVISNNSGCGGAALSRRRFGETV
                     ARINTSFPHWFCDNFKKYNNNEDALPVDQHELIALIAPRPVYIASATEDLWADPRGEF
                     LAAKGADPVYKLLGTEGLPVEDMPPPDQPVMGRIGYHLRTGKHDITAYDWEQYIKFAD
                     KHFGRTTSKVE"
BASE COUNT          490 a          630 c          634 g          510 t
ORIGIN      
        1 gcctcgtttg ttgtgctccc gatcgtggtc attgacgtga ttcgcgtgag caacaaattt
       61 gtggggcccc tactccggtt gcggcaatca gtcagaagcc tcgctgcggg ccaggatgtg
      121 gagcctctaa gattccggaa gggagatttt tggcgcagtt ttgcagacga ctttaacgcg
      181 ctcgtttcac ggataaaaca cctagaaact gctgcccacc aaagtaaagg caccagtgcg
      241 gacccatggc aggaagacag gccactggcc gaaagcggtg caactccggg cccaacaccg
      301 gtgggcgata ttctcgcggt agggggaaat ttggacggcc ctgtcatgca cagggtgtga
      361 cgggcttgac tactcttcca ccggtcgaga cgccctctag tccttaatgg tctctgttgc
      421 cgcctttggc cgggtgccag acggcgagcg acttgacttc tcagttggcg ggttgaataa
      481 tcgaggtggc attttagtct gttggaactg gcgcgcgacg ccagctctcc accagcagtc
      541 cgaggttttg gaccgggagg tcggccatga tgagtcagac gggagaggtt agcaaaactt
      601 cgataaaaga aatcctgctg gcaaagggaa gtcacgttta tacgattgat cccgatgcta
      661 ccttgcagga cgttgtcgaa gaattggtcc aacacaacat cggtgcgcta gttgtgtgcc
      721 accgtgatcc agaccggggt gaaaaaatcc tgggcatcat caccgagcgc gatatcttgc
      781 ggcgacgtgc ctattccacg ggcccgctaa gcgaggtccg tgtttcggaa gtcatgtcgc
      841 ggaacctcat tacagcccag ccggacgaca cggtcgcaca cgccttgcaa cttatgacga
      901 ttcatcgtgt ccgtcatctt ccgattgtcc tggatgggca attagtcgga attatctcga
      961 tcggggatgc ggtaaaggcc caactcgagc accttatgct cgagaatcgt ttcatgaagg
     1021 attacatcca aggctgaacc cgaatctcgt cctactcgac cttggatgtc gtacgcccga
     1081 aatgcttatc ggcaaatttg atatattgtt cccagtcata cgctgtgata tcgtgtttgc
     1141 ccgttcgcaa gtggtaacca atacgaccca ttaccggttg gtctggcggg ggcatatctt
     1201 ccacgggtag gccttccgtt cctaggagtt tatacactgg atctgcgccc ttggctgcga
     1261 gaaactcccc gcggggatca gcccaaaggt cttccgtcgc actggcgata tagacgggtc
     1321 gcggagctat cagggcaatc agctcatgtt gatcaaccgg taaggcgtcc tcattgttgt
     1381 tgtacttctt gaaattgtcg cagaaccaat gaggaaaact ggtattgatt cgcgcgacgg
     1441 tttcgccgaa acgccggcgg cttagggccg ctcctccaca acctgaattg ttggaaatga
     1501 caatcgcaaa gcgttcgtct tgcgcgcccg cccacaaagc cgtcttgccc aaacgcgaat
     1561 ggcctaggac ggctacgcgc ttttcgtcga tgtcatcgtc ggtctcaaag tagtccatgg
     1621 cgcgactgag tccccatgcc caggccccaa tcgaacccca ctcgtcgggt gcgggcctgg
     1681 tttgaccctc tttgtaaaag agcggatgca caccgttttg gaatccatcg tcgaaatcgg
     1741 gatcaatgtc cccgtagtaa gccgtggcaa caccgtagcc gtgttccaaa accatctcga
     1801 tgggccaacg actggccgcc gtaccacggc ctgcctcggt ggcacggtta ttgacgacac
     1861 cttcgccggg tcgcatccaa ctcgttggaa ttgagatcgc tgggtcggga tggaccgcat
     1921 gatttccccg aaagttcaag ccaagaaaca cgggcaccgg tcggggcgcc tcttttggca
     1981 agtagatgag aagatccatt ccagggcctt catctttctt agccgagaaa tgaatccgga
     2041 cctcctttcg cacagcttta ccccccaggg cattgtcgtc gcgactttta agaacaaaag
     2101 acaaatctgc agggcggccc ggagaccggc cataaacgtg ggtttggaac agttgcagca
     2161 gttctggccg acgcttttgc atccatgtgg cggcgtcgcg aaccaccgtc ccgtcctcgc
     2221 agacaagcgg atcgggcaag gtatacgcgg gcaccttgct ctcg
//
LOCUS       DAOPTR010000683         2258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_37837_length_2258_cov_3.035860, whole genome shotgun sequence.
ACCESSION   DAOPTR010000683 DAOPTR010000000
VERSION     DAOPTR010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37837_length_2258_cov_3.035860"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..>2258
                     /locus_tag="PKI05_12125"
     CDS             61..>2258
                     /locus_tag="PKI05_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52992.1"
                     /translation="MARPGETPRRASRSVVRKIVNEHLGDFDEQVWPVNLAILVFAGF
                     VTGIVLATTENFFDPRLLHNGFFRLGVIAVLVIGLLFSTARLKAPTARRIQFCVVLSL
                     FLHMILAVFLREQYLKLVAFLREKAAGSTTVEEVVTIPDYVESVFAEPGALSEYEKPA
                     TTAMPTERESMTEITPQAPPVPSVSEIAEESRPENVLPEEVTPLAAERQSLQTPERLI
                     EQLQSHNRQIDREATPLREEAPLQRPVELSPSKPRDMVPQDQITRQDVLPESVILRQS
                     PSASEVVLPAAPPRATTERNVENRATPPGPRQAISSQTLVSSQPEMSTPSLGGKPTSR
                     QLNPGDPALNPQATPTPLVAPVSDVPQESAWTPPAVVRSPLPTENTAQVGALAPSNPP
                     RRNPANQRDLLTSTANLDPSLAQLPAGGAASGPSSLEARESALAPTAPLANLDAAALR
                     LGTPAADDLSAATAAMIRDSGVSRLGQAQGASTGGGEGETSRAGLDTLATQPRSLRNT
                     GKTSGLPSLVSPEVGNVGVGGPVAGTGESGAPSSSGVGDKLAFRPGQLGAGQVPLLEV
                     HSDADGGPSGLLSGQGVGGVPGPTVPAMVRGSADEALGTGAGALAATGLRPGRGRASG
                     GVGLPGAGLAELAGGPDGLTGQGGGSSHGVKAGGETGSVQGGVSVADQGGFEIAAGPV
                     GETPRLLGSTGGGDETGSVTSSLLAGMPSGMPGRPERLVVSPIGRRGLIGR"
BASE COUNT          424 a          686 c          725 g          423 t
ORIGIN      
        1 tgtgatggct gggggatttg gagtggttcg ctcagttttg tagctcgatt gtgtgcccca
       61 atggcacgtc caggtgaaac accaagacga gcgtcgaggt cagttgtccg taagatcgtc
      121 aacgaacacc tcggcgattt tgatgaacag gtttggccgg tcaacctggc catcctggtt
      181 ttcgcgggct tcgtgacagg gattgtccta gccaccacag agaatttctt cgatccacgg
      241 ctactccaca acggattttt ccgccttggg gtgattgccg ttctcgtgat tggtcttctt
      301 ttcagtacgg ccaggctcaa ggcaccgacg gcacgacgca tccagttttg cgtggttctc
      361 agcctgtttc tccatatgat cctggccgtg ttcctccgcg agcaatattt aaaactggtg
      421 gcgtttctgc gtgaaaaggc agccggaagt acgacggtgg aggaggtggt caccatcccc
      481 gattacgtcg aaagtgtgtt cgccgagccg ggggccttgt cggaatatga aaaacccgcg
      541 actacggcca tgcccacaga acgcgagtca atgacggaga tcactcctca ggccccccct
      601 gtcccctctg taagtgagat tgcggaggaa tcccgtcctg agaacgtgct tcctgaagag
      661 gtcacaccct tggcggctga acgccagtcg cttcaaaccc cggaacgact catcgaacaa
      721 ttgcagtccc acaatcgtca gatcgatcga gaggcgacac cgcttcgtga ggaggccccg
      781 ctccaacggc ccgtagagtt gagtccctcc aagccgcgtg atatggtacc ccaagaccag
      841 atcactcgcc aggacgttct tccggaatcc gtgatcttgc gacaatctcc gtccgcttcg
      901 gaggttgtct taccggccgc tccgccacgg gcaaccaccg aaagaaacgt cgaaaatcgt
      961 gcgaccccgc ctggacctcg tcaggccatc tcttcacaga cacttgtgag tagccaaccg
     1021 gagatgagta cgccttcact gggtggcaaa ccgacgtcac gccaattgaa tccaggtgat
     1081 cctgccctca atccgcaggc aacgccgacc ccgctggttg cgccggtttc cgacgtaccc
     1141 caggaatcgg cctggacgcc acccgccgtg gtgcgatcac ccctacctac cgaaaacacc
     1201 gcgcaggtgg gagccttagc cccgtccaac cccccgcgac gcaatccggc gaaccaacgg
     1261 gatcttttga cctcgacggc caacttggat ccttctttag cccagttgcc ggcaggcggc
     1321 gcggcgtccg ggccgtcatc gcttgaagca cgtgagtcag ccctcgcccc caccgctccg
     1381 ctcgccaatt tggacgccgc cgccctgcga ttgggaacac cggcggccga cgatctgtct
     1441 gcggccacgg ccgccatgat ccgcgattcc ggcgtttctc gattgggcca ggcgcaaggt
     1501 gcgagcacgg gaggcgggga gggggagacc tcccgggccg gcctcgatac gctggcaact
     1561 cagccgcggt cgctgcgaaa tacgggcaag acgtcgggac ttccctccct cgtttcgccc
     1621 gaagtgggta acgtcggggt cggtggaccg gtggccggca ctggagagag cggggcacca
     1681 agctcaagcg gagtgggcga caaattggcc tttcgaccgg gtcaactggg agcagggcag
     1741 gtaccgcttc tggaagttca ttcagacgcg gacggcggac ccagtggcct tctaagcggt
     1801 caaggcgtgg gcggcgtacc cggaccgacg gtcccggcaa tggtcagagg ctcggctgac
     1861 gaggccctcg gcacgggggc gggcgcgctc gcagcaactg gcctgcgtcc tgggcgaggc
     1921 cgcgcctcgg gaggggtagg ccttcctggg gcagggttgg cggagctagc gggaggaccc
     1981 gatggtctga ccggtcaggg aggcggctcg tcgcacgggg taaaagcggg cggcgagacg
     2041 ggttcggttc agggaggagt gtccgtggct gatcaagggg gatttgaaat cgcagcgggc
     2101 cccgttggtg aaacaccccg gttattggga tcaacgggtg ggggagatga aacgggcagt
     2161 gtaacttcat cgttgctggc cgggatgccc tcgggtatgc cgggacggcc ggagcgactg
     2221 gtagtgagtc ccatcggccg acgtgggctc atcgggcg
//
LOCUS       DAOPTR010000684         2255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_37902_length_2255_cov_3.236818, whole genome shotgun sequence.
ACCESSION   DAOPTR010000684 DAOPTR010000000
VERSION     DAOPTR010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37902_length_2255_cov_3.236818"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PKI05_12130"
     CDS             complement(<1..702)
                     /locus_tag="PKI05_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52993.1"
                     /translation="MEIPRMRDTCYPGLRVAVVGRLAAMSRQELQKHLSERGLFLISD
                     LSDNPDWVVFGEYDLAWADDPRIASLLDKPSPPEVIPESELWRRLGLTPDCGNTKSLF
                     TPAMIADLVKVPVREVRRWYRLGLLTAVQVVHRLPYFDYQGLLDARRLAELWESGIPS
                     QRLNRVLENVCRAIPRWKKRLPQLSFVVHGETILLQSGDKLIDYRGQLQLPLVFEDDG
                     PSKKDCETVLRIADHI"
     gene            complement(738..>2255)
                     /locus_tag="PKI05_12135"
     CDS             complement(738..>2255)
                     /locus_tag="PKI05_12135"
                     /inference="COORDINATES: protein motif:HMM:NF018757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HOA52994.1"
                     /translation="IGYANRTIEVQLFFETEPGKPEMVEKQRVTVETSDERIPVRFRY
                     IPEATGERKVSIVVPPQPGEALITNNEQSTLVKVLAGGIKVLYVEGALRVEQRFLRKA
                     LDASPDIHVDYVRLDLRSREGRPADMDRRLQPGGYDVYFLGDVDATLFEGQELASLAD
                     AVSKGAGLLMLGGLQSFGAGGYAETPLAEVLPIRMNRFERQQPNEPVREDLHITGPVR
                     MVPTPVGLKHFVMTLSPSPAENQRLWESIPPLDGANRWAGIKPGAIVLAEDDRGGPLL
                     VAQFYGQGRVVAFAGDSTWRWCLHGFETAHKRFWRQLTLWLAKKEDTQQNPVWIHLAQ
                     RQIPLGERLFFEVGAGDPLGEAITDVTWQVEIVSPSGKRHPVAVRQQGKNWQGEFAET
                     AEPGDYQLEVQASQNGQVLGQDKARFLVVKRDVEMDQPASDPGLLAALASASGGRSCA
                     PEEFPEVLAELRQRLKEMEVPVATRVTLWDRWPVLLIFVGLTSLEWFLRKRWGLV"
BASE COUNT          528 a          687 c          558 g          482 t
ORIGIN      
        1 gatatgatcg gcaatcctca agacggtctc gcaatccttc ttgcttggac catcatcttc
       61 aaaaaccaga ggtaactgga gttggcctcg gtaatcaatc agtttgtctc cagactggag
      121 aagaatcgtc tcgccgtgca ccacaaaaga gagttgcgga aggcgttttt tccaacgtgg
      181 gatcgcacga cagacatttt cgaggaccct attcaaccgt tgggacggga tccccgactc
      241 ccacagttcg gccaatcgtc gggcatcaag aaggccctga tagtcaaagt atggcagacg
      301 atggacgact tgcaccgccg tcagtaatcc caggcgatac caacgccgca cctccctgac
      361 ggggaccttt accaagtcgg caatcatagc cggcgtgaac aaggacttcg tattcccaca
      421 atcaggtgtc aatcccaaac gcctccagag ctccgattca gggatcactt ccggcggaga
      481 gggcttatcc aaaagtgatg cgatccgcgg gtcgtctgcc caggccaggt catactcccc
      541 aaacacaacc caatcagggt tgtcggaaag atcagagatc aaaaaaagtc ctcgctctga
      601 caaatgcttc tgcaactctt gccgggacat cgccgccagc cgccccacaa cggccacacg
      661 cagtcccgga taacatgtgt ccctcattct gggaatttcc atttccaaca tcacaagtca
      721 ggtaaccgtc ccgccaatta caccaaaccc cagcgctttc ggagaaacca ttctaggctg
      781 gtcaaaccga caaagatgag caagaccggc caacgatccc aaagggtcac ccgagtggct
      841 acggggactt ccatttcctt taacctctgc cgaagttccg ccaaaacttc tggaaactct
      901 tcgggtgcgc atgatcggcc tcctgaagcc gaagccagag cggctaacaa accggggtca
      961 gatgccggct gatccatctc cacgtcacgc ttgacgacca aaaaccgcgc tttatcctgg
     1021 ccgagcacct gcccattttg agaggcctgc acttccagtt gataatctcc cggctcggct
     1081 gtctctgcaa attcgccttg ccaattcttg ccttgctggc ggacggcaac aggatgacgc
     1141 ttaccacttg gcgaaacgat ttctacttgc caggtcacat cggtgatcgc ttctccgagc
     1201 gggtcgccag cccccacttc aaagaatagg cgttcgccga gaggaatttg gcgctgggcg
     1261 aggtgaatcc aaacggggtt ctgctgcgtg tcctctttct tggccaacca tagggttaac
     1321 tgccgccaga accttttgtg ggccgtctca aacccgtgga gacaccatcg ccatgtggag
     1381 tcgcccgcaa aagctaccac gcgtccctgt ccatagaatt gggcaacaag gagcggtcca
     1441 ccacggtcgt cctccgccaa aacgatggct ccgggtttga tgcctgccca acgattggcc
     1501 ccgtccagtg gtggaatcga ttcccacagt cgttgatttt ccgcgggaga aggcgacaac
     1561 gtcatgacaa aatgtttcag cccaacaggc gtggggacca tccgcacagg tcctgtgatg
     1621 tgaaggtctt ctcgaacggg ttcgttaggt tgttgccgct cgaaacggtt catacggatg
     1681 ggtaagacct ccgccagcgg cgtttctgcg tatccgcctg caccaaaact ctgcaatcct
     1741 cccaacatca aaaggccagc cccttttgaa accgcatctg ccagcgacgc caattcctgc
     1801 ccttcaaaca gggttgcatc cacgtcgccc aaaaagtaaa cgtcatatcc ccccggttgc
     1861 aaacggcgat ccatatccgc cgggcgtccc tcccggctgc gaaggtccag tcgcacatag
     1921 tccacgtgaa tatcgggcga ggcatctaga gctttccgga ggaaccgttg ttctacgcga
     1981 agggcacctt cgacatataa gaccttgatt ccccctgcaa ggactttgac cagcgtactt
     2041 tgctcattat tggtgatcaa agcctcgccg ggctgcggtg ggacgacaat acttaccttt
     2101 cgctcacccg tcgcttcggg gatataacga aaccggacag ggatgcgttc gtcagaggtt
     2161 tcgacagtaa ccctctgctt ttccaccatt tcgggtttac ccggttccgt ttcaaagaaa
     2221 agttgcacct caattgtgcg attggcgtaa ccgat
//
LOCUS       DAOPTR010000685         2250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_38005_length_2250_cov_2.780866, whole genome shotgun sequence.
ACCESSION   DAOPTR010000685 DAOPTR010000000
VERSION     DAOPTR010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38005_length_2250_cov_2.780866"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            922..2202
                     /locus_tag="PKI05_12140"
     CDS             922..2202
                     /locus_tag="PKI05_12140"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOA52995.1"
                     /translation="MRRLIFACLVSVTVGALAAVAAVKSGLTGGDRYMIDQPQDRQAA
                     FAEPWFPQGSEGPGSLPTWTASPTPWTVSTAQGQFVFTQSAPGWEAFLPPVGQYTPDE
                     IVNIAVYEKVNKSVVNIRTKSIQEGALFFMNIITEGEGSGSVIDRQGHILTNYHVVEN
                     ARAISVTLHDGMAYDAQLVGADPPSDIAVLKIDAPSEKLFPVTFGDSTNLKVGQRVFA
                     IGNPFGLERTLTTGIISSLNRSLPNPRSQRMLKQMIQIDAAINPGNSGGPLLDSHGLV
                     IGMNTAIASRTGESAGVGFAIPVNTIARVVPQLIRTGRVIRADAGILQVLETDQGILI
                     ASLKPGGAAERAGLQGFRLTKRTRRQGPLVFESTVIDRSTADLIIAVDGKPVKTVDDL
                     LTVLESKQPGEKAIFTIIRQGRQMDVPLILDAEN"
BASE COUNT          532 a          561 c          647 g          510 t
ORIGIN      
        1 cggcgatgga caagtggtgg gatcgcaacc gggacggctt gtgcccgaat caggcaatca
       61 aaatagccgg ccggaagttc tgcgacatat cctttcgatt ccaccttgcc gaaatccatg
      121 ttcagaccaa tgagaggctt gcgacgcatt gtttacctcc ttgtttaagc gatcctttgc
      181 ctgagaaacg agacatgacc acggaacggg gcttaccgat tgaactcgtc aaacgaagaa
      241 cgttcaagag taagtctgaa tagcacagtc cttaagatgg tcgtttgggg gagaggcacg
      301 ttggggatgg gcctggaaca atagcttcgg aagagtccgg aaaaggcgaa acaagaaaga
      361 attgctaact cttatcacca gcatcgcctc catgcgagtt gtgtgggcta tcgacgacct
      421 ctcgtttgtt agactatgtc agcagataga gtttcaacgc ttttgccgac gtgacgcgct
      481 cttgtcgtcc tgcctcatcc atccctgcgt aggccaaatg tgacgcgtgc catcccactg
      541 ctgacgtgat acggatgact tggcacgctc gcgaagcccg ttttcatgaa gaatgtagcg
      601 acctatgttc gcttgtcaac acaacctcaa gattttggcg aatttcgttg gcgggcacaa
      661 aaagttgtgg tgacggcatt gcttgtattg ttcgcgccaa cgacgtatct gcgtcagtcg
      721 tgggtcgctt cgtgtgaaag gcgagcccaa tttgccattc tggctttgac gcacatcacc
      781 tttgcgatga aaaggccgtg cggtaaagta ggagaacgcg gaggcaaaca ccaagacatg
      841 atcttcgcgt atgatggggg tgcggacctc tctggtaaac aacgggatga ttgatcaagg
      901 gactagcgta agggcgtcgt tatgaggcgt ttgatatttg cctgtcttgt cagcgttacg
      961 gtcggcgcgt tggcagctgt ggccgccgtg aagtccggtt tgaccggtgg tgatcggtac
     1021 atgatcgatc aacctcaaga tcgccaagcg gcctttgccg aaccatggtt cccacaaggg
     1081 tccgagggtc ccggaagcct accgacgtgg actgcatcac cgactccctg gaccgtaagc
     1141 actgcacaag gccagtttgt cttcacgcaa agtgcaccag gctgggaggc ctttctgcct
     1201 ccagtgggac agtacacccc agatgagatc gtcaacattg ccgtctatga aaaggtcaac
     1261 aagagcgtcg ttaatattcg aaccaagagt attcaggaag gggccttgtt cttcatgaat
     1321 atcatcacgg aaggggaggg gagtggtagc gtcatcgatc ggcagggaca catcctcacg
     1381 aactaccatg tggtggaaaa tgcccgggct atctctgtga cgcttcatga tgggatggcc
     1441 tatgatgccc agctagtggg tgcagatccg ccgagtgaca ttgccgtcct caaaatcgat
     1501 gcaccatctg agaagttgtt cccagtgacc tttggtgact caacaaatct gaaagtgggt
     1561 caacgggttt tcgccattgg taacccgttt ggattggaac gaacgttaac gacgggaatc
     1621 atctccagcc ttaatcgttc gctacccaat ccacgtagtc aacgcatgct gaagcaaatg
     1681 attcagattg atgccgcaat caatccggga aattccggtg gtcccctctt ggacagtcac
     1741 gggttggtta ttggcatgaa tacggccatt gcgagtcgaa ccggtgagag cgccggcgtg
     1801 ggatttgcca ttcctgtgaa cacgattgcc agggtagttc cccagttgat ccgcacggga
     1861 cgtgtcatcc gggctgatgc cggaattctt caagtgttag aaacggacca gggcatctta
     1921 atcgcctccc tcaaaccggg cggggcagcg gagcgggcag ggctgcaagg attccgactg
     1981 acgaaaagga ctcgccggca ggggccgctc gtcttcgaat cgaccgttat cgatcgctcg
     2041 acggccgact taatcattgc ggttgatgga aagccagtaa agacggtgga tgatcttctg
     2101 accgtcttgg agagcaagca accgggcgag aaggcgattt tcaccatcat tcggcaagga
     2161 cggcaaatgg atgtccccct gatcctcgat gccgagaatt gatccccttg ggaaggaaaa
     2221 cggtgttgga gggaccggta aaggccatgg
//
LOCUS       DAOPTR010000686         2249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_38024_length_2249_cov_3.347311, whole genome shotgun sequence.
ACCESSION   DAOPTR010000686 DAOPTR010000000
VERSION     DAOPTR010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38024_length_2249_cov_3.347311"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..387
                     /locus_tag="PKI05_12145"
     CDS             250..387
                     /locus_tag="PKI05_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52996.1"
                     /translation="MKRTYFARIVGVKLLWQKAIKTQRQEGRGRWSLRIASPARAVER
                     P"
     gene            384..1190
                     /locus_tag="PKI05_12150"
     CDS             384..1190
                     /locus_tag="PKI05_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA52997.1"
                     /translation="MSYDASAQALRCPFCGSTELKSQPDGVSLTPKYVVPFEVTREQA
                     RGLLYQRMRSGFFRPSDLVQRSAITEMMAVFVPYWIFSANTHTYWTADSSDVPFHARG
                     EWRPLFGEQRHQHEAIRIVASKVLSWEESEGLGQYDLSRGVPPEQVDLDNVVVEQFAM
                     PRKYARTHARQLIENAEKVYCANSLLRGRFRNLRVNVKIEQMRGEPILLPVWILAYRY
                     RDRVYRFVVNGQTGRAYGAFPTSPWKVLLAVVLFLLAAVIVLALLAQLAG"
     gene            1309..2109
                     /locus_tag="PKI05_12155"
     CDS             1309..2109
                     /locus_tag="PKI05_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOA52998.1"
                     /translation="MNERREIPHPWRLADLTYYDVRQEHYEVAVLPLGATEPHNLHLP
                     YSMDTREGDLVGEMICSAAWRQGAKVILLPTIPYGTETNQRAFPFAMNVNPSTLHAFL
                     ADLIESLVHQGILKVVLLNSHGGNDLKGFLREMYGKSPAKLFLCNWYTVFHDVYHQIF
                     NEPDHHAGEMETSLALAYWPELVARRTDGTLLADSGVKQPTRFEAVNRGWVSITRPWH
                     LLTTNAGAGNPHAASAEKGQRVMEILVERLAKFLVELSQSPLDERFPF"
BASE COUNT          491 a          564 c          633 g          561 t
ORIGIN      
        1 caaaaacgag tgatcgacaa cttgggaacg tgttaacatc gtcactcccg catcgcagga
       61 ctgagtagaa gccgatcctt tctttccacg cttgtcgcca ttataacaaa tctgtgcaag
      121 gaaaaaagaa tgaatgagca cgggattcta tcggagcagc ctttcggccc aagcggttgc
      181 gccttctcat tttaagaatt gctcaaatca tgttggaacg tcagtgccct agctgcgcga
      241 gcctgcttga tgaagaggac ctattttgcc cgaattgtgg gcgtgaagct cctctggcag
      301 aaggcgatca agacgcagcg ccaagagggc cgaggccgat ggtctttaag aatcgcttca
      361 cctgccaggg ctgtggagcg gccatgagtt acgacgcctc ggcgcaggcc cttcgctgtc
      421 ccttttgcgg ctccacggaa ttaaagtcac aacctgatgg cgtaagtttg acccccaaat
      481 acgtcgttcc atttgaggtc acaagggaac aggcccgcgg gctgttgtac caacggatgc
      541 gatcggggtt tttccgcccc agtgatttgg tccagcgatc tgccattacg gaaatgatgg
      601 ccgtctttgt tccctattgg attttttccg ccaacacgca cacctactgg acggcggaca
      661 gttcggatgt cccatttcac gcccggggag agtggcgacc gctgtttggg gagcagcgtc
      721 atcagcacga ggcaatcagg atcgtggcca gtaaggtcct ttcctgggaa gaatcggagg
      781 gcctgggaca gtacgatctt tcgcgcggtg ttccgccgga acaagtcgat cttgacaatg
      841 tcgttgttga acaattcgct atgccgcgaa agtatgctcg cacccatgcc cggcagttga
      901 tcgaaaatgc cgagaaggtc tattgtgcaa actcgttgct tcgcggtcga tttcgcaatc
      961 ttcgcgtgaa tgtcaagatc gagcagatga gaggggagcc gatcctcctt cctgtttgga
     1021 ttttggctta tcgctaccgg gatcgtgtct atcgttttgt ggtcaatggt caaaccggtc
     1081 gtgcctatgg tgcctttccg acttcacctt ggaaggtcct tctggccgtt gtgcttttcc
     1141 ttctggccgc tgtgatcgtg ttggcactgt tggcacagct tgccggttaa tttcagcaca
     1201 cgccgcgagc catctgttta ttgttcggcg tccttgttcg acgataacgc cgaggcagta
     1261 aaatggtcca atggtcgttt tcgcggttca gtggcaaagg acaatcgtat gaacgagcgg
     1321 agagagattc ctcatccttg gagattagcc gatttgacat actatgacgt ccgtcaggaa
     1381 cattacgagg tggccgtgtt gccgcttggg gcgacggagc cacacaacct ccatctgccg
     1441 tacagtatgg acacgcggga gggagacctg gtaggcgaga tgatctgctc ggcggcgtgg
     1501 cgtcaagggg ccaaggtcat tttgctgccg accatcccgt atgggactga gaccaaccag
     1561 cgagcatttc cctttgcgat gaatgttaac cccagtaccc ttcacgcttt tttggcggac
     1621 ttgatcgaga gcctggttca ccagggaatc ctcaaggtgg tcctgctcaa cagccacggc
     1681 ggcaatgact tgaaaggctt tttgcgagag atgtatggta aatccccagc caaactcttt
     1741 ctttgtaatt ggtacaccgt ttttcatgat gtgtatcatc aaatcttcaa tgagcctgat
     1801 catcacgccg gcgaaatgga aacatcgttg gccttggcgt attggccgga gttggttgca
     1861 cggcgaactg atgggacact tctcgccgat agtggtgtga agcaaccaac gcgttttgag
     1921 gccgtcaatc gtggttgggt cagtatcacg cggccgtggc atcttttgac gaccaatgcc
     1981 ggcgcaggaa atcctcacgc ggcatcggcg gaaaaaggcc aacgggtgat ggagatcctt
     2041 gtcgaacggc tggccaagtt cttggtggag ctttcccaat cgccgctcga tgagcgattt
     2101 ccgttttgaa aatcctgtgg ctctttaaaa aatcggccag aaaaaatgct gcgaaccgca
     2161 ccggcattac ttgtgtcaga cttgaatgta ggggctgggc aagatgttgt aacgaaatcg
     2221 tttttagtgc gtcaatttaa agagagtgt
//
LOCUS       DAOPTR010000687         2241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_38184_length_2241_cov_2.983989, whole genome shotgun sequence.
ACCESSION   DAOPTR010000687 DAOPTR010000000
VERSION     DAOPTR010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38184_length_2241_cov_2.983989"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PKI05_12160"
     CDS             complement(<1..369)
                     /locus_tag="PKI05_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008681722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOA52999.1"
                     /translation="MAAQRKPQQEPTEPRGERLQKVLAAAGIGSRRQCEELIRMGRVE
                     VDKKTITELGFRVDPRRQKICVDGVPIKLEPHVYYAVYKPDGVVCTNRDPAGRTRVID
                     LVPETNLRLFPVGRLDLHSEG"
     gene            complement(705..777)
                     /locus_tag="PKI05_12165"
     tRNA            complement(705..777)
                     /locus_tag="PKI05_12165"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(742..744),aa:Phe,seq:gaa)
     gene            1190..>2241
                     /locus_tag="PKI05_12170"
     CDS             1190..>2241
                     /locus_tag="PKI05_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53000.1"
                     /translation="MVLRRFTYVGLCCVIAVGTLFSIAGRSEAWGHEAANVIEVDVNK
                     PYPPLSEYIYGQFIEHLGRCIYGGIWAEMLQDRKFFYAVGAKGSPWKVIGRQECVTMD
                     RNQPFVGVHSPRVSLADDGTRCGLSHGELGIVSGKKYTGYIWMRGNTSAPVEIALIWG
                     EGPSERATHAVSSLTPDYTKYEFEFEAGADSDNARLEITSTGKGYFQIGTLSLMPADN
                     IHGMRADTIALLKELNSPIYRWPGGNFVSGYDWRDGIGDRDRRPPRKNPAWQGIEHND
                     FGIDEFMVFCRVLNTEPLVVVNTGLGNLQMALEELEYANGPADSPMGRLRAKNGHPKP
                     YGVRYWGIGNEMYGNW"
BASE COUNT          522 a          599 c          619 g          501 t
ORIGIN      
        1 gccttcgcta tgaaggtcca atcgccccac ggggaatagc cggaggttcg tttcaggaac
       61 aagatcaatg acccgcgtcc ggcctgcggg gtcacgattc gtgcaaacca caccatccgg
      121 cttatatacg gcataataaa catgtggctc caacttaatc gggacaccat cgacacagat
      181 tttctgtcgt cgcgggtcca cgcggaaacc caattccgtg atggtcttct tatcgacctc
      241 gacgcgcccc atgcgaatca attcttcgca ctgccggcga ctgccaatac cggccgccgc
      301 caggaccttc tgaagtcgct cgccccgggg ttctgtcggt tcttgttgag gtttacgttg
      361 cgccgccatg ctattggcct tatttgttac gcggaggaaa aacagccttg aatcccacaa
      421 gggagaatgc cacacggcca tctcggcacg tgcaaggtgc tggcaccagg gaaagcctat
      481 tttaagaaaa tgagtcgtgt gccatcccat cattgtagag gcattcaaac agatagctat
      541 cccaggatgc tgacgggctg tcgagaataa ccaagataag aacacaacct accgactcgc
      601 cccggcagat gccccgcttt gcctgtatga aacagaacgc ctagacccct agtgctcagc
      661 cctcgtatgg ccgtcacaat acccccaaag cctcgactcc ccagtggaca ggggcgggat
      721 cgaaccgccg acacctggat tttcagtcca gtgctctacc aactgagcta cctgtccaaa
      781 tgccgcgtca gcggcgagcc ttcgctatgg cattatctta caacgcgcaa ccgtccattg
      841 tcaaagggga gttgcggggg cctcgacgat aaaggaaacc accgactgca gcaagcgggt
      901 acgaagcgaa ggacctcggt ctcttgtccc atggagcgct cctccgtttc cccaaaacgc
      961 acatcgacgg cccactttgg gcggtaccgg aagtgaatgg aacgatcctc tgccttccga
     1021 aaacggaaat ccttgtctgg taccacagcc attacgctgg cccaaattct gcccacttgt
     1081 ggctgcacat agtggtctat aaactgacga tgttcgtgac tgtcacgatt tccagtcgtt
     1141 caagtaacag tgcatccttg ggaaaaactg gagtaaggga agctgactta tggtgctgcg
     1201 aaggttcacc tatgtggggt tgtgttgtgt gattgctgtg ggaacgctgt tctccattgc
     1261 tggtcggtcg gaagcttggg gacacgaggc cgcaaatgtg atcgaggtgg acgtgaacaa
     1321 gccttatccg cccctctcgg agtacatcta cggccagttt attgagcatc tggggcgatg
     1381 catctacggc ggcatttggg ccgaaatgct gcaggaccgc aagttctttt atgctgtggg
     1441 agcaaaaggt tccccctgga aagtgatcgg tcgccaagag tgtgtgacca tggatcgaaa
     1501 tcagccgttt gttggtgtcc atagccccag ggtctcactg gctgatgatg gcacacgatg
     1561 tggtttgagc catggagagt tggggatcgt ctcagggaag aaatacacgg gctatatctg
     1621 gatgcgtggt aacacaagcg cccctgtaga aattgcattg atttggggcg agggaccatc
     1681 ggagcgtgct acgcatgccg tgagttcatt gactcctgat tacacgaaat acgaattcga
     1741 attcgaggct ggtgctgaca gcgacaatgc acgtttggaa atcacttcca caggcaaggg
     1801 gtattttcag atcgggacgc tgtcattgat gccggcagat aatatccacg ggatgcgggc
     1861 cgacacgatc gccctgctta aagagctgaa ctcccccatc tatcgatggc cgggtggtaa
     1921 cttcgtcagt ggttacgatt ggcgcgatgg cattggcgac cgtgaccgtc gtccacctcg
     1981 aaagaacccg gcttggcaag gcattgaaca caatgatttc ggcattgacg aattcatggt
     2041 cttctgccgc gtcctcaata ccgagcctct tgtagtggtc aacacaggct tgggcaatct
     2101 gcaaatggct ttggaggaat tggaatacgc caacggtccc gccgattctc cgatgggaag
     2161 acttcgggca aagaacggtc acccgaaacc ctatggcgtg cgatattggg gcattggaaa
     2221 tgagatgtat ggtaactggc a
//
LOCUS       DAOPTR010000688         2239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_38227_length_2239_cov_3.921703, whole genome shotgun sequence.
ACCESSION   DAOPTR010000688 DAOPTR010000000
VERSION     DAOPTR010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38227_length_2239_cov_3.921703"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PKI05_12175"
     CDS             <1..495
                     /locus_tag="PKI05_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53001.1"
                     /translation="QSLREIVVQLRSLTMARPTSQAADDAGHTHPDVSQWEERIRQLE
                     EDLNEAQRQREVLETEVMVLRHRAMEWLEMLADQRRQLLEERNRWSTELRQFRRLVEV
                     LLDHHMEITPEHQEDQEPNGPPRVIPYEPAEARRTGTGDAFLDSLSAQFEQLRRDFDR
                     RRGK"
     gene            complement(558..1346)
                     /locus_tag="PKI05_12180"
     CDS             complement(558..1346)
                     /locus_tag="PKI05_12180"
                     /inference="COORDINATES: protein motif:HMM:NF016005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOA53002.1"
                     /translation="MSGEDVVNPEGDGLAEDKAVSLEDLARAFAEATGQSFDLPQGDE
                     TTEIDSIGEPSEEAAVPDAVAASAEADVPATPKSILEAILFVGHPHNQPLSPAQAAEV
                     MRGVQPEEIAHLVTELNAGYEATNRPYRIVFEGGGYRMKLLPEFQTLREGFYGKVRQV
                     RLSQQALEVLAIVAYKQPITVKEVNQMRGRASGALLAQLVRRDLITVSTPVDLTGKKG
                     VPQYRTTQRFLDVFGLQSLEDLPQLGEASLIKPLDDSTPSTPGS"
     gene            complement(1386..1721)
                     /locus_tag="PKI05_12185"
     CDS             complement(1386..1721)
                     /locus_tag="PKI05_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53003.1"
                     /translation="MRLGNLVTTRSNVYAIWVTIGYFEVQPVAEVMQPDPNVPDNFLI
                     NGMSFSPAEIAEIYPDGYMLGQELGSDTGEIKRHRAFFLFDRTIPVGFRRGYDLNVDQ
                     GLLIKRIIE"
BASE COUNT          524 a          622 c          625 g          468 t
ORIGIN      
        1 cagtctctcc gcgagattgt cgtgcaactc cgctcgctga cgatggctcg tccgacttcc
       61 caggccgctg atgacgcggg acacactcat ccggatgtga gccaatggga ggaaagaatc
      121 cgccagcttg aggaggacct gaacgaggcc cagcggcagc gcgaggtctt ggagactgag
      181 gtcatggtgc ttcggcaccg ggccatggaa tggctggaaa tgctggccga ccaacgtcgg
      241 caacttcttg aagaacgcaa tcggtggtct accgaactgc ggcaatttcg gcggttagtg
      301 gaagtccttc tcgatcatca catggagatc acaccggaac accaggagga ccaagaaccc
      361 aacggcccac cgcgcgtgat cccctacgaa ccggcggaag ctcggcggac gggtacgggc
      421 gacgcctttc tcgattcttt gtccgctcaa tttgaacaac tcagacgaga ctttgaccgc
      481 cgccgcggaa agtaacggct tcttattgtc ctgatccgag cgggaaattc tgctcgatga
      541 cgtctgggct tgatcggcta cgatccaggc gttgatggcg tactgtcatc caatggcttg
      601 atcaatgatg cttcgcccaa ttggggtaga tcctccaggc tttgtaggcc aaacacgtcg
      661 aggaaccgct gcgtggtgcg atactgagga accccctttt tccctgtgag atcaacaggt
      721 gtggaaaccg taatgaggtc ccgcctcacc agttgcgcga ggagggcacc actggcacgg
      781 ccgcgcatct ggtttacttc ctttaccgtg atgggctgct tataagcgac aatggccagt
      841 acctcaagag cctgctggga aagcctgact tgccggacct ttccataaaa gccctcccga
      901 agtgtctgga attccggaag caatttcata cgataaccgc cgccttcaaa aacgattcga
      961 taaggacgat tagtggcctc gtacccggcg ttgagttccg tcaccaagtg ggcgatctct
     1021 tccggctgca cacccctcat gacttccgcc gcttgagccg gagaaagcgg ttgattgtgc
     1081 ggatgaccaa caaagaggat cgcctccagg atactcttgg gcgttgccgg aacgtccgcc
     1141 tccgcggaag ccgcgaccgc atctggcaca gccgcttcct ctgagggctc gccaatagaa
     1201 tctatctcag tcgtctcgtc gccctgggga aggtcgaacg actgaccggt ggcctcagca
     1261 aaggcccggg ctaggtcctc cagagagact gccttatcct cggcgagacc gtcaccctca
     1321 ggattcacaa catcctcacc agacatgcct gtcaacctcc ttgcccagcg tgttgtgacg
     1381 atccttcatt caatgattcg tttgatcaag aggccttgat ccacgttaag atcgtacccc
     1441 cgacggaagc ccaccgggat ggtgcggtca aagaggaaga aagcgcggtg ccgtttgatt
     1501 tcgcccgtat cgctgcccaa ctcttgaccg agcatgtaac catcgggata aatctcggca
     1561 atctcggccg gagagaagct catcccgttg atcaggaaat tatcaggaac gttggggtca
     1621 ggctgcatca cttcggccac tggctggacc tcgaagtagc cgatcgtcac ccaaatggcg
     1681 taaacgttgg accgcgtggt cacgaggttg cccaggcgca taagatctcg atagtggaag
     1741 aacgggttac gagaatcacc ttgcgcaggg gcaaataacg gctttgtgtt atttagagct
     1801 ggccttaata gggaaacgtt aacgccaaaa atatcacccg actgagaacg gatcaaatca
     1861 ttcaactcgg gaacgggagg aacccaggga gcgctgtgaa atcttctgaa gggatagcca
     1921 ctgcggacgg caactgtaaa gctggtgaaa cttgaccaac acgcgagttc cttaaatcat
     1981 cgaattgaac agctgtagat attttgcccc ataccgcgga attaagcacc gtattgagat
     2041 taacctttcc cggctcgcgg tattgatcga tgcgatcaaa aggcggaaca aaaccgcgtg
     2101 aacttccagg gaggggaacc tgtctttggc tattcacgaa gactggggaa gttctcagga
     2161 aatcgagaat ggccgccatg tctggcacag ggtctggcgc aggatctgcg tcctttcgct
     2221 cccgtaggaa gaaatcgat
//
LOCUS       DAOPTR010000689         2237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_38284_length_2237_cov_2.613199, whole genome shotgun sequence.
ACCESSION   DAOPTR010000689 DAOPTR010000000
VERSION     DAOPTR010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38284_length_2237_cov_2.613199"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            346..888
                     /gene="hslV"
                     /locus_tag="PKI05_12190"
     CDS             346..888
                     /gene="hslV"
                     /locus_tag="PKI05_12190"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOA53004.1"
                     /translation="MRIHGTTVLTVRRGDSVAIGADGQVSLGNAILKGDASKIRRLAD
                     GKVLTGFAGSTADALALYERFEAKLKEFPGNLPRAATELAKEWRTDRALRRLEAMLIA
                     VDAKHTLLLSGTGDVLQPSDGVLGIGSGGMYAVAAARALLRFTSLSAREIVEAALDIA
                     ADIDVYTNRNIIVEEISCRV"
     gene            876..2213
                     /gene="hslU"
                     /locus_tag="PKI05_12195"
     CDS             876..2213
                     /gene="hslU"
                     /locus_tag="PKI05_12195"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOA53005.1"
                     /translation="MSCLTPHQIVAELDKYIVGQTMAKRAVAVALRNRWRRQQLPPEI
                     RADIGPKNILMIGPTGVGKTEIARRLARLTGAPFVKVEATKFTEVGYVGRDVESMVRD
                     LVDNAISLLFQEYREKLKDEAEKRVENRILDILMKSPEDWDGLVASDEGARKNRVRTK
                     LRQLLREGVFDDRTIEIVTEKKELPFLVTGGGLEQIDIDFQSWAERLFAGKKSQRREV
                     TVKEAKKLFFDEEVEALINKDEIQAKAIELTENSGIIFIDEIDKIASVGVTHGPDVSR
                     EGVQRDLLPIVEGTTVKTRYGYVKTDHILFIAAGAFHRAKPSDLMPELQGRFPIRVEL
                     SDLTKDDFVKILTETETALVKQYQALLRPENVTLIFDSDAIELIAEYAYRVNQTTQNI
                     GARRLYTVMEKLLEDVSFDAPENISGEFRVTRDYVAAKLGDVIKDEDLSRFIL"
BASE COUNT          526 a          523 c          673 g          515 t
ORIGIN      
        1 agctcagatc aggaatggct gacgtggcaa atcacctcgt cccattcgta tgctcgccgt
       61 tggcgttttc tcactgtcgt ggagaatgac gtgatcgcgg cgtctgtaac aggtctaatc
      121 gcctaattgc cggaagtacg tttccttttg ggggctaacc tttctgcgaa atggggagtc
      181 aatgcaaagg tttggttggg aagtacgatc acgcgttatt ctggcgtccg cttgacgctc
      241 ccgcgtgttt ggcgctaact taatgaattg gtccgatcaa ttgcgaggac gccttcagta
      301 tccgccggct gcgccggcgt attttcttca cgcttaggtg gtgctatgag aatacacggg
      361 acgacggtcc tcacggtccg gcgcggcgac agcgtggcca ttggggccga cggtcaagtg
      421 agcctgggaa acgccatcct gaagggcgac gcgtcgaaga ttcgtcgctt agcggacgga
      481 aaagtcttaa cagggtttgc cggaagtacc gccgacgcct tggccttata tgagcgattc
      541 gaggccaaac ttaaggagtt tccgggcaac ctgccgcggg cggccacgga gcttgccaag
      601 gaatggcgga ccgatagggc cttgcgccgt ctggaagcca tgctgatcgc cgtcgatgcc
      661 aagcatacac tattattgag cggaacaggt gatgttcttc aaccgtccga tggggtactc
      721 ggcatcggat ccggggggat gtatgccgtt gcggctgctc gtgctcttct gaggttcacc
      781 agtttgtcgg ctcgcgaaat cgtggaggcc gcgctggata ttgcggcgga catcgatgtt
      841 tacaccaatc ggaatatcat tgtggaggaa atttcgtgtc gtgtttaaca ccacatcaaa
      901 ttgtcgctga acttgacaaa tatatcgtgg gacaaacgat ggccaagcgg gcagttgccg
      961 ttgcgctgcg aaatcgttgg cgaaggcaac aactgccgcc ggagattcgc gcagacattg
     1021 gcccaaagaa tatcctcatg attggcccca cgggtgtggg gaaaactgaa atcgccagga
     1081 gattggcccg gttgacaggt gccccatttg tcaaggttga ggcgacaaaa ttcacggaag
     1141 tggggtatgt cggtcgggat gtcgagagta tggtgcgtga cctcgtcgat aacgccattt
     1201 ccttgttgtt tcaggagtat cgtgaaaaac tcaaggatga ggcagaaaaa cgcgtcgaga
     1261 accgcatcct cgatatcctg atgaaatcac ccgaagattg ggatggcctt gtcgcttctg
     1321 acgagggggc gagaaaaaac cgggttcgaa cgaaacttcg tcaattactc cgggaagggg
     1381 tctttgatga ccgcaccatc gagatcgtca cggagaaaaa ggagttgcct tttctcgtga
     1441 ctggtggcgg cctggaacaa atcgatatcg acttccagag ttgggcggag cggctctttg
     1501 ccggaaaaaa gtcgcaacgt cgcgaggtga cagtcaagga agcgaagaag ctcttttttg
     1561 atgaagaagt ggaggccctg attaacaagg atgaaatcca ggccaaggcc atcgaattga
     1621 cggagaattc gggcatcatt ttcattgacg aaattgacaa aattgcctcg gtcggagtga
     1681 cgcacggtcc cgatgtttcc cgggaggggg tccaacgcga tctactgccc atcgtggaag
     1741 ggacaacggt gaagacccga tatggttatg tgaagaccga ccacatcctg tttatcgctg
     1801 cgggggcgtt tcatcgggcg aagccgagcg acttgatgcc tgagttacaa ggccgttttc
     1861 ctatccgggt ggaactctct gatctgacaa aagatgattt tgtcaaaatc ctgacagaga
     1921 cggaaacggc gttggtgaag caatatcagg cacttctgcg gccggaaaac gttacgctga
     1981 tatttgattc ggacgcgatt gaattgattg cagagtatgc ttatcgcgtg aatcaaacca
     2041 cgcagaatat tggcgcccgc cgactttata ccgtcatgga aaagttactc gaagacgtga
     2101 gcttcgatgc gcctgaaaat atttctgggg aatttcgggt gacgcgcgac tacgtggctg
     2161 caaagctggg ggacgtgatc aaagacgaag acttgagtcg tttcatcctg tagcatgatc
     2221 gaccttgagt gggactg
//
LOCUS       DAOPTR010000690         2234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_38355_length_2234_cov_3.216613, whole genome shotgun sequence.
ACCESSION   DAOPTR010000690 DAOPTR010000000
VERSION     DAOPTR010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38355_length_2234_cov_3.216613"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="PKI05_12200"
     CDS             complement(<1..720)
                     /locus_tag="PKI05_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOA53006.1"
                     /translation="MSVIVSRALPDVRDGLKPSQRRILVAMNDLNLGPGAARVKCAKI
                     CGDTSGNYHPHGEGVVYPTLVRMAQEWNMRYVLIDKQGNFGSIAGLPPAAMRYTEARL
                     SPYAAMMLEDLNLDTVDFVPTYDERHLEPTVLPSKFPNLLVNGATGIAVGMSTEIPPH
                     NLSEICDALVRLIDDPDVTIDELLEIVPGPDFPTGGIICGRRGIRQGYLTGHGTIVIR
                     ARADIEEYRDNRYRIVIREIPF"
     gene            1166..1248
                     /locus_tag="PKI05_12205"
     tRNA            1166..1248
                     /locus_tag="PKI05_12205"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1199..1201,aa:Leu,seq:caa)
     gene            1732..>2234
                     /locus_tag="PKI05_12210"
     CDS             1732..>2234
                     /locus_tag="PKI05_12210"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HOA53007.1"
                     /translation="MNVKAMTFLLGKTLLLIGLLIARDAGSGTAFASNPLVLWYNSPA
                     EKWTEAVPIGNGRLGAMVFGKTDCERIQINEDTVWAGSPHDYSHPGAAKYLPEIRRLL
                     FEGKQEEATALAMEHFMSVPLRQMPYQPCCDLYLDFGENAKVSSYRRELDLSSATAIT
                     SYQVDGVT"
BASE COUNT          482 a          586 c          620 g          546 t
ORIGIN      
        1 gaatgggatt tcccgaatga caattcgata ccgattatcg cggtattcct cgatgtcagc
       61 ccgtgcccga atgacaatcg ttccgtgccc ggtcaaataa ccctgtcgaa taccgcgacg
      121 cccacaaata atgccacccg tggggaagtc gggaccgggg acaatctcca aaagctcgtc
      181 aattgtaaca tccggatcat caatcaaacg gaccaatgca tcacaaatct ccgagagatt
      241 gtgcggagga atttcggtgg acattcccac cgcgatgccg gtggccccat tgacgagcag
      301 attcggaaac tttgagggca gtactgtcgg ttccaaatgc cgctcgtcat aagttggaac
      361 aaaatccacg gtgtcgaggt taaggtcctc cagcatcatt gccgcgtagg gggaaagccg
      421 ggcttccgta tatcgcatcg ccgctggagg caaccccgcg atggacccga aattcccttg
      481 cttgtcgata aggacatacc gcatgttcca ttcctgggcc atacggacga gcgtcggata
      541 gacaactcct tccccgtggg gatggtagtt cccgctcgtg tcgccgcata tctttgcgca
      601 cttcacgcgg gctgctccag gacctaggtt gaggtcattc atcgcaacaa gaatccgccg
      661 ctgagagggc ttgagtccat cccgaacgtc gggtaatgcc cgactgacga ttacgctcat
      721 cgcgtacgtg aggtagctgg acttcagctc gtcttcaata gccagcggaa tgactcggct
      781 gcgggtttcc ccctggggag attgcccacc tggtgtgccc gtgctattgt cgttttcttc
      841 aaactctgta tctgacataa gatatcaccg tgcccaagaa ggttttcttc gcggtttgac
      901 ccatttgcgg ctgtaataag ccccttacgg gccatcatgg ccgtaaacag ttagcatatc
      961 agatttcctc ccttccgaca atcgcggggt aagcgcatta gttgatcgca agtattgatc
     1021 tagccgttac ttgtgatcaa catttgcgcc tcaggcaaga taggcgagtt gactcgatgg
     1081 agaacgaggg ggcacgttgg ggtggggctt gtcggtcgcc aacttagtga cgatattatt
     1141 cagtatccga gatgcgttaa ggactgccgg agtggcggaa tggcagacgc gctggactca
     1201 aaatccagtg gccttcgggc cgtgtgggtt caactcccac ctccggtact tcgcttcttc
     1261 gggctgggtg tcttctctgg ccggttcgtg gtgcttagtc agcattccat tcgaagggct
     1321 ttttgaccag tagcttgcgc actttagaaa accgcgcaag attggtttct ctctcttgtg
     1381 gtccggtttt ttctggacca tcttttttgg aacgtttccg ttggcacttc gcagtaactc
     1441 caagcggaaa gacaggttcc gcagggtttt ccccctttaa ctcgtggaca aacctccggg
     1501 ctgcaaaaga acgcggttgg tttttctccc ttggtgaaaa gtgtcttacg gcgacaccaa
     1561 aagctttcct tcagtctatt gacaccgcac ggtaagacgt ccacgataca atggtcttcc
     1621 cacaaggggg tagcgaccag gtttcccgca aggtcacacg ggcatgcgcc tctaggggac
     1681 attgtgtggt tgtgcgtggg cgaattctct gatagccgaa ggtttgccgg gatgaatgtt
     1741 aaagcgatga catttctgtt gggaaaaact ttgctgttga ttggcttact gattgcgcgg
     1801 gatgcaggga gcgggacggc gtttgcctcg aacccgctcg tcttgtggta taactcgccg
     1861 gcggagaagt ggacggaggc cgtgccgatt ggtaatggcc gcttgggggc gatggtcttt
     1921 ggtaagaccg actgtgaacg gattcagatc aacgaggata cggtttgggc ggggtcgcca
     1981 catgactatt ctcatcccgg agctgccaaa tacctaccgg agattcgacg cctgttgttc
     2041 gaagggaaac aagaagaggc aaccgccctg gctatggaac acttcatgag cgttcctttg
     2101 cgacaaatgc cgtatcaacc ttgttgcgac ctgtacctcg atttcggtga aaacgcaaag
     2161 gtctcgtcat atcggcgcga actcgacctc tcgtcggcaa cagccattac cagttaccaa
     2221 gtcgacgggg ttac
//
LOCUS       DAOPTR010000691         2232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_38393_length_2232_cov_3.293523, whole genome shotgun sequence.
ACCESSION   DAOPTR010000691 DAOPTR010000000
VERSION     DAOPTR010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38393_length_2232_cov_3.293523"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PKI05_12215"
     CDS             complement(<1..320)
                     /locus_tag="PKI05_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HOA53008.1"
                     /translation="MLRNWLDFSDRVLNGHKLTRDDARAVLSSPDTDILEILAAAYRV
                     RRHFHGVRMSLNFLINAKSGLCGEDCAYCSQSRVSTAPVPKYPLVGEQEILEGAKVAV
                     ANKAS"
     gene            739..1797
                     /locus_tag="PKI05_12220"
     CDS             739..1797
                     /locus_tag="PKI05_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53009.1"
                     /translation="MGKSQAEEKPTTGKCPPLVMATDLFRPHDDPDDHWDLATGYALA
                     FRGLVDLKAVLIGFPRPNAAKDPDVLAVSQMNYITGLKVPVIVGSPHPYAKDMTLHGD
                     EATLRRFGSLFRILEQSPEPVIISVAGSCRDIATASKLRPDLFARKCAAIYLNAGASR
                     PSVSQKDGMEWNVRLDPAAYHAMFELPCPIYWMPCFEDVRKFQVAEYATYFQFQQGEI
                     LSQVSPSVRKFFAYVFEKDHPQPPAADWLRYLLAEENEPLLSAMAQERRNMWCTGGFI
                     HAAGLMVTPEGELVPANSGRSAVFEFVPITVTKSEGGVVEWQPGEGEQRFIFRVTDQQ
                     RYTPAMTRALLTLLRALP"
     gene            complement(1961..>2232)
                     /locus_tag="PKI05_12225"
     CDS             complement(1961..>2232)
                     /locus_tag="PKI05_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53010.1"
                     /translation="RQNGSEPLAKENLHTIEFHGASPDEVQRSPRTATLPIQPPRGLR
                     RRLHETITRNHCSRTMEQILGKTYRRVQQHGTFSWRFPATDKLAS"
BASE COUNT          485 a          614 c          628 g          505 t
ORIGIN      
        1 gaggccttgt tggccactgc gactttggcg ccctcgagaa tttcttgctc ccccaccaat
       61 gggtatttgg gcacgggagc cgtcgaaacc ctggactgcg aacaataagc acaatcttca
      121 ccacacaggc cgctcttggc attgattaag aaattgagcg acatccgcac gccgtggaaa
      181 tgccgccgta cacgatacgc tgcggcgaga atttctagaa tgtctgtgtc tggcgaagac
      241 agaaccgccc tcgcatcgtc acgggttaac ttatgaccat tgagaacgcg atcgctgaag
      301 tcaagccaat ttcgtaacac gctgtttgga tcctccgttc agacacttgc taccgataca
      361 acgccccaac ggacgagtct caatcggata caaccgacgc ctcgccatta agagtctatc
      421 cccaaaggtc cccctctccc gccgaacgcg tgaggggaga ttatgggata ggttcaaagt
      481 tgccacgaaa accaccaaac cgtcattcta ccggaatggg tccactcgaa aaggtcgctg
      541 cgcgccggct ggtctttggg ccgctagaag gctataacat gatcgccgag tatgaggcga
      601 tttgccacaa tgaggaccgg ttgaaccgtt tagcggctag tccgcgaggt gtgacgaacc
      661 atgcaaccat ggaacttgcc gaggatttct cgtcgaacag ctttggctgg attgggtgta
      721 ggaggatatt tgctgcgaat gggcaaatcg caggcggaag aaaagccaac aacgggaaaa
      781 tgtccgccct tggtcatggc caccgacctg tttcgtccgc atgatgatcc ggacgatcac
      841 tgggacctgg ccaccggcta tgccctggca tttcgggggt tagtcgatct caaggctgtt
      901 ttgatcggtt ttccgcgacc gaatgcggct aaagaccccg atgtcctggc agtctctcaa
      961 atgaactaca tcaccgggtt gaaggtaccc gtcatcgtcg gctcaccgca tccgtacgcg
     1021 aaagatatga cgctccacgg agatgaagcc acacttcggc gttttggcag tctatttcgg
     1081 attctagaac agtccccaga gcctgtaatc ataagtgttg ccggttcgtg ccgcgatatt
     1141 gccaccgcgt cgaaacttcg tccggacctt ttcgctagga agtgtgcggc aatttattta
     1201 aatgccggtg ccagtcgccc aagtgtttcc caaaaagatg gtatggaatg gaacgtccgt
     1261 ttggatccgg cggcatacca cgccatgttt gagttgccct gtcccattta ctggatgccc
     1321 tgttttgagg acgtacgaaa gtttcaggtt gcagaatatg caacatattt tcaattccaa
     1381 caaggtgaaa tccttagtca agtgagcccc tcggtccgca agttcttcgc ctatgtgttc
     1441 gagaaggacc atccccagcc gccagccgcc gattggcttc gatatctcct ggcagaagaa
     1501 aatgagccgt tgctttccgc catggcacag gagcggcgga acatgtggtg cacgggtgga
     1561 tttattcacg cggcaggcct catggtaact cccgaaggag aattagtccc tgcgaacagc
     1621 ggcaggtcgg cggtctttga attcgtcccg atcacagtta ccaagtccga aggaggcgtg
     1681 gtggagtggc aaccaggaga gggcgagcaa cgattcattt tccgagtcac ggatcaacag
     1741 cgttacaccc cggcaatgac gagggccctc ctgaccttgt tacgcgcgtt gccgtgatgg
     1801 cttggagcct atgccctaat ctccccctca cgcgttcgac gggagacggg tcgggcgtgg
     1861 gggtggctcg aatcgctgca ggttttccca atggattccg acgttcgctt gccctcaccc
     1921 caaccctctc ccagagggag agggaacttt gggataggct ctaactagcg agcttatccg
     1981 tcgccggaaa tcgccaggaa aacgtgccat gttgttgcac ccgacggtag gtttttccaa
     2041 gtatctgctc cattgtgcgg gagcagtggt tgcgggtaat cgtctcgtgg aggcgacggc
     2101 gcaaccctcg agggggctgt attgggaggg tcgccgtcct cggcgaccgc tgtacctcat
     2161 ccggactggc cccgtggaac tcgatcgtgt ggagattttc cttggcgagc ggttctgagc
     2221 cgttttgtcg cc
//
LOCUS       DAOPTR010000692         2228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_38480_length_2228_cov_3.662218, whole genome shotgun sequence.
ACCESSION   DAOPTR010000692 DAOPTR010000000
VERSION     DAOPTR010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38480_length_2228_cov_3.662218"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1766
                     /locus_tag="PKI05_12230"
     CDS             <1..1766
                     /locus_tag="PKI05_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOA53011.1"
                     /translation="IRAFGEFVAGDQIVVGLIIFIIIVVIQFVVITKGATRISEVAAR
                     FTLDGMPGRQMAIDADLNAGIIDEREAQRRREEITRQADFFGAMDGASKFVRGDAIAG
                     IIITLINIIGGLFIGMVQMGLPLSQAAALFTKLTIGDGLVSQVPAFLISLAAGLLVTR
                     SSTEVNLPAEFLRQMFGRPRTLAVAGAFLGILIFTQLPTIPLLTVGGACAALAVTLSR
                     KTRKQKQSEEARKQAEATKPPEERIEDYLLVDPMELEIGVGLIRLADPKRGGDLLDRI
                     HRIRKNIAGDIGIILPKVRIRDNFQLDQHQYTIKIAGAPVATGMVYAHLLMAMDSGMT
                     TGKVPGIETKDPAFGAPALWIEPSQRERAEMYGYTVVEPAAVIATHLTETIRRHADEI
                     LTRDATKHLIEELKKSSPAVVEDLIPNVMRLAEVQQILQMLLREQVPIRQLGLILETL
                     GDYAPRTKDPILLTEFVRARLARTISTRYRDAEGRMLVVTLDPALEDRIRAGFDHTER
                     GLFIRMSPQMMERIARGIAQEVSKLTTQHRPPVVLVSPQIRPALKEMMTPYLPNLVVL
                     SYNEITRDTRIESIAMVTDVA"
     gene            1849..>2228
                     /locus_tag="PKI05_12235"
     CDS             1849..>2228
                     /locus_tag="PKI05_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53012.1"
                     /translation="MDAMDVRTFRASTMHEALAIVRRELGPDAAILRTREIRVRRLFG
                     LLPGRAEIEVTASVNVNVPSRLPPSSSPLGGLNRECRESVVETGLSLERASAETRALV
                     MRTGAFHPEVQHRLENMEQMLRTL"
BASE COUNT          484 a          607 c          641 g          496 t
ORIGIN      
        1 ttattcgcgc atttggggaa tttgtggcag gtgaccaaat cgttgttgga ttgattatct
       61 ttatcatcat tgtcgtgatt cagtttgtgg tcatcacgaa gggggcaacc cgcattagcg
      121 aggtcgccgc ccgattcacg ttggacggga tgccaggccg tcaaatggcg atcgatgccg
      181 atctcaacgc ggggattatc gacgagcggg aggcgcaaag acgccgtgag gaaatcaccc
      241 gccaagcaga cttttttggg gcaatggacg gtgccagtaa gttcgtacgc ggcgacgcca
      301 tcgccggcat catcatcacg ttgattaaca tcattggcgg gctgtttatc ggcatggtcc
      361 agatgggcct tcccctctct caggctgctg ccctctttac gaagctcacc atcggcgacg
      421 ggcttgtcag ccaggttccc gcgttcttaa tctccttggc ggccggtctg ttagttaccc
      481 gcagcagcac ggaggtgaat cttccggcag agttcctccg gcagatgttt ggcaggccgc
      541 ggaccttggc agtggccggc gctttcttgg ggattttgat cttcacacag ttacctacca
      601 tcccgctatt aaccgttggt ggcgcctgtg ctgcattggc cgttacttta tcacgaaaaa
      661 caaggaaaca gaagcaatcg gaagaagccc gaaaacaggc cgaggcaacc aagcccccgg
      721 aggaacggat cgaggactac ttgctcgttg atcctatgga actggagatc ggggtgggct
      781 tgattcgtct agccgatccg aagcggggcg gcgatctgct cgatcgcatc catcgcattc
      841 gtaaaaacat cgccggagac atcgggatca ttttgcctaa ggtccgcatt cgtgacaatt
      901 ttcagctcga tcagcatcaa tacacgatca agattgctgg tgctcccgtg gcaaccggga
      961 tggtttacgc ccacttgctg atggcgatgg attcggggat gaccacggga aaggtcccgg
     1021 ggattgaaac caaagatccc gcctttgggg cccctgccct ttggatcgag ccttcccagc
     1081 gcgaacgggc tgagatgtat ggatacaccg tcgttgaacc ggcggccgtg attgctactc
     1141 acctcacgga aaccatccgc cgtcatgcgg acgaaatcct cacccgcgat gctaccaaac
     1201 acctgatcga ggaactgaag aagagctctc cggccgtcgt tgaggacttg atccccaacg
     1261 tgatgcgact cgcagaggtc caacaaatcc tccaaatgtt gctccgcgag caggtgccca
     1321 tccggcagtt ggggctaatt ctggaaacgt taggggatta cgccccgcgc accaaggacc
     1381 ccatcctgtt gacggaattc gtgcgggcaa ggctcgcgcg gacaatctcc acgcgatatc
     1441 gcgatgccga gggccgtatg cttgtggtca cgctcgaccc cgccttggag gatcgcatcc
     1501 gcgcgggatt cgatcatacc gagcggggac tgttcattcg gatgtcaccg caaatgatgg
     1561 agagaatcgc ccgcggcatt gcccaggagg taagtaagct gacgacccaa catcgaccac
     1621 cggtcgtttt ggtcagtcct cagattcggc cagctctgaa agaaatgatg accccgtatt
     1681 taccgaattt ggttgttctt agttacaacg aaatcacccg cgacacgcga attgagtcca
     1741 tcgccatggt aacagatgtc gcgtaaccga gccttgagga gcaaagagct gtctcacaag
     1801 ctgaaatcat agatgccaac gagtttcgat ttgtcaggat gaaatcgcat ggatgctatg
     1861 gacgttcgga cgtttcgcgc tagcacgatg cacgaagccc ttgccattgt gcgaagagag
     1921 cttggtcctg atgctgccat ccttcgcaca cgggaaatcc gtgtccgtcg tttgtttgga
     1981 cttttgccag gacgtgcgga gatcgaagtc acggcctcgg tgaacgtcaa tgttccaagt
     2041 cgtctgcctc caagttcttc tcctcttggc ggactgaacc gggaatgccg agagtcggtc
     2101 gtggagaccg gcctgtccct agaacgcgcg agtgcggaga cacgcgcgtt ggtcatgcgg
     2161 acgggggcct ttcatcctga agtccagcat cggttggaga atatggagca aatgttgcga
     2221 acgttatg
//
LOCUS       DAOPTR010000693         2226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_38521_length_2226_cov_3.967296, whole genome shotgun sequence.
ACCESSION   DAOPTR010000693 DAOPTR010000000
VERSION     DAOPTR010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38521_length_2226_cov_3.967296"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            364..1206
                     /locus_tag="PKI05_12240"
     CDS             364..1206
                     /locus_tag="PKI05_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOA53013.1"
                     /translation="MRVLFAISLVFAIVGCCASWTSGEEWVSGKIWEEPPIVTPGECG
                     SPPSDAIILFDGKDLSQWEGGENWKIENGYAIAQKTGITTKEQFGDCQLHLEFATPEV
                     IEGSGQGRGNSGVYLMGRYEVQILDSYENKTYFDGQCGAIYKQHPPLVNASRKPGEWQ
                     TYDIIFTGPRFDEAGNLLKPAYVTVIHNGVVVQNHFEIEGSTAWHVPPKYEPHGEKGP
                     IHLQFHGNPVRFRNIWIREIRELTYQQKGQEPEKKEPEKNEAEKAEAEKTEPEKKAEN
                     GQNQ"
     gene            1678..>2226
                     /locus_tag="PKI05_12245"
     CDS             1678..>2226
                     /locus_tag="PKI05_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HOA53014.1"
                     /translation="MSIRVAILGATGYTAVELVKLLLRHPEVTITALTSRQEGNAPIA
                     SVHPSLVGRIQANLENLSAAEVAARAECVFSCLPHCASAEVIPELLKAGCRVVDFSAD
                     YRLASAQVYEDWYGHRHPDPDRLGKVPYGLPELFREAIPSADLVANPGCYPTSAILPL
                     APLLKEGLIAREDIIVDSKSGVS"
BASE COUNT          465 a          582 c          678 g          501 t
ORIGIN      
        1 ccgggcggcc ataacgtcaa ccgtaggggc aaccccctgt ggttgccccg tttgccgtag
       61 cgccgcgggt ttacgtttgg ccggttgccc agatcgtttg gtttccacca cgatccgtag
      121 gttgtttgcg ttccggggca ggcacagggg cctgccccta ccagtttcaa tgggctcctt
      181 ctcgcgcagg tccacagctg cccggtagct tgcgtctttt taagaacaga tggactctgc
      241 ccggcatgtc accatctcct tgacgtcgta aacacgtcta atcatgatga tcagtttgtg
      301 cacggtcgtg atggccgtgc cggttggctt ccgtgattgg gttttgtgag aggaggtttt
      361 gttatgcgcg ttctgttcgc gataagtttg gtgtttgcca ttgtcggatg ctgcgcttcc
      421 tggacgagtg gcgaggaatg ggtcagtggc aagatttggg aggaaccgcc tatcgtaaca
      481 cctggcgagt gcggttcgcc gccgtctgac gcgatcatcc tcttcgatgg caaggacctg
      541 tcgcagtggg agggcggaga gaattggaag atcgaaaacg gctatgcgat tgcccaaaag
      601 accggcatca ccactaagga acagttcggc gattgccagc ttcatctcga gtttgccacg
      661 cccgaggtca tcgaaggctc tggccaaggt cgcggcaata gcggtgttta tctcatggga
      721 cgttacgagg tgcagattct cgactcgtac gagaacaaga cttatttcga tggtcagtgc
      781 ggggcgatct acaagcaaca tccgccactt gtcaatgcct cccgcaagcc gggcgaatgg
      841 cagacgtacg acatcatctt tacgggtccc cgtttcgatg aggcggggaa tctccttaag
      901 ccagcctatg tgacggtcat ccataatggc gtggtggttc aaaaccattt tgaaatcgaa
      961 ggttccacgg cctggcacgt tccgcccaag tacgagccgc acggcgaaaa gggcccgatc
     1021 caccttcaat tccacgggaa tcccgtcagg ttccggaata tctggattcg ggagatccga
     1081 gagttgacct accagcaaaa aggccaggag cctgaaaaga aagaaccgga aaagaacgaa
     1141 gcagagaagg ccgaagccga aaagacggaa ccggaaaaga aggctgaaaa cggccagaat
     1201 cagtgacgtt atcctggcgt ttccatcgca acaatcgcca gcccccatcg taagatgggg
     1261 gtgtcttttt tcccggcagg aaatgccgtc gcaaaaagtg gcaggtcggg cgtgataaag
     1321 gatcatgggc tggggtaggc gaatcagatg atgggaggac cgcgcggtgt ggtggccggg
     1381 ctcgatgatg gaatatcaaa cggcacgtca ccggaggcga tggcgcgcgt tcttcccatt
     1441 gtccttccac accgcaggtg tgtttccaaa ttggccatcg gccaaccacg aaggttgtgc
     1501 ctaccgtggt gcggcgggca ccggcgagcg acaaatcccc gaaccttcgc tcggctactg
     1561 taccgtaaat acaaggaggg gtatattgca ggttgatcgc gtttcccacc gtgccacagt
     1621 aagaccggcg gggagcgggg tgcgagtaat ctttttggga ttgagtatta agcagctatg
     1681 tcgatccgag tcgccatttt gggtgccaca ggttatacag ccgttgaatt agtgaagctc
     1741 cttctgcgcc atcctgaagt cacaattacg gcgctgacca gtcgtcagga gggaaatgcc
     1801 cctattgcga gtgtccatcc atcccttgtt ggtcggattc aggcgaattt ggagaatctt
     1861 tcggcggcag aagtggccgc tcgggctgag tgcgttttta gctgtctccc ccattgtgcc
     1921 agtgcagagg tcatccccga attactgaaa gcagggtgtc gcgtagtcga tttcagtgcg
     1981 gactatcggc tggcctccgc ccaggtttat gaggactggt acggccatcg gcatcccgac
     2041 ccggatcgac tgggcaaggt gccctacggt ctgccggagt tgtttcggga ggcaattccc
     2101 tcggccgatc tggtggccaa tcccggctgt tatccgacgt cggccatctt gccgctggcc
     2161 ccgctgctga aagagggttt gatcgcgcgg gaggatatta ttgtcgattc taagagtggc
     2221 gtctcg
//
LOCUS       DAOPTR010000694         2225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_38550_length_2225_cov_3.430415, whole genome shotgun sequence.
ACCESSION   DAOPTR010000694 DAOPTR010000000
VERSION     DAOPTR010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38550_length_2225_cov_3.430415"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /locus_tag="PKI05_12250"
     CDS             <1..1386
                     /locus_tag="PKI05_12250"
                     /inference="COORDINATES: protein motif:HMM:NF003700.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HOA53015.1"
                     /translation="EYKTMLRTDEILSTIRMLHAEHLDVRAVTLALNVEDCAAPNVDH
                     MCRKLRDKITSRAARLVEICDRVGAKYGIPVVNKRLAISPVSTLLAGHGKGAALQVAK
                     TLDATATACGVDFLGGFTALVHKGISPGDQVVMESLPEVLASTQRVCASVNVASRKAG
                     INMDAVREMGFIIHRTAQLTADRKGFGCAKLVVFANIPEDNPFMAGAYMGAGEPEATV
                     NIGVSGPGVVGSALKRRLERGDKVTLGDLAEEIKITSFRVTRVGELIGREVAAELGVA
                     FGIVDLSLAPTPTVGDSIGEIIKTLGIEKIGAPGSTAAVAMLNDAVKKGGLFASSSVG
                     GLSGAFIPVSEDAALAQAVGAGHLVLEKLEAMTSVCSVGLDMIAIPGDTPPETIAALI
                     ADEMAIGVINDKTTACRLIPVPGAVAGEWVDFGGLFGAAPVIEVRNSGASAAFIRFGG
                     RIPAPLQSLGN"
     gene            complement(1487..>2225)
                     /locus_tag="PKI05_12255"
     CDS             complement(1487..>2225)
                     /locus_tag="PKI05_12255"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOA53016.1"
                     /translation="AGQLTPQHYVVETLVTTRLLGRIARKAGVRVIDDLMVGFKWIGG
                     VMDYEGPDRFLIGAEESYGFLIGDHARDKDGAVAAMVLCELAAELKARGQTLVGRLEE
                     LFREHGLHEEMAFSIDLPGERGMQLMKDLMQKFRTNPPKELGGQRVVSVHDLNQPIGV
                     DPSQEWEYAMAAARRGNVVLLHLDEKGDNRVAIRPSGTEPKCKFYVFGYLPPSALGDL
                     RQARDDLQRQLKLIAEDLKAFSFQGVS"
BASE COUNT          475 a          623 c          654 g          473 t
ORIGIN      
        1 gaatacaaaa cgatgcttcg cacagatgag attctttcga cgattcggat gctccatgcc
       61 gagcatctgg acgtacgtgc ggttacattg gcgctcaacg tggaagactg tgcagccccc
      121 aatgttgacc acatgtgccg taaattgagg gacaagatta cctcacgggc ggcacgactg
      181 gtggagattt gcgatcgtgt gggggccaag tacgggattc cggtggtgaa taaacggctg
      241 gcaatctcac ccgtttcgac acttctcgcc ggacacggga aaggggcggc tctccaggta
      301 gcaaaaaccc ttgatgccac ggccacggcg tgtggagttg acttccttgg gggttttacg
      361 gccctcgttc acaaaggcat ctcgccaggg gatcaggtag tgatggaaag cctaccggag
      421 gtcctagcga gcacacagag ggtatgtgcc tcggtcaacg tggcctcgcg gaaggctggg
      481 atcaatatgg atgccgtccg agaaatgggt tttatcatcc atcgaacggc acaactgaca
      541 gccgaccgca aaggctttgg atgtgccaaa ctcgttgtgt tcgctaacat ccctgaggat
      601 aaccccttca tggcgggtgc ctacatgggg gcaggcgagc cagaagcaac tgttaatatt
      661 ggcgtatcgg ggcccggtgt tgttggtagt gccctcaaac gacgcctcga acgcggcgac
      721 aaagtcacgc tgggggatct tgctgaggaa atcaagatta caagcttccg tgttacccgg
      781 gtgggagagc ttattggccg cgaggtggcc gcagaactcg gcgtcgcctt tgggattgtg
      841 gacctctctc tggctccgac accgacggtg ggtgacagta tcggcgaaat catcaagacc
      901 ctggggatcg aaaaaatcgg ggccccgggc tcaacagcgg cggtcgccat gctcaatgat
      961 gccgtgaaaa aggggggact ttttgcctcc agctcggtcg gcggattgag cggggccttt
     1021 atccccgtta gtgaggatgc cgctcttgcc caagctgtcg gggcgggaca tctcgtctta
     1081 gagaagctcg aagccatgac gtccgtatgt tccgtcgggc ttgacatgat tgccatcccg
     1141 ggagacacac cgccagaaac gatcgccgcc ctgattgccg acgaaatggc aatcggcgtc
     1201 atcaatgaca agacgacagc ttgccgattg atcccggtgc cgggcgccgt ggcgggcgag
     1261 tgggtcgatt ttggcgggct gttcggtgct gctcccgtta tcgaagttcg taattccggg
     1321 gcctccgcgg ccttcatccg gtttggggga cgtatccccg ccccacttca gtcacttggg
     1381 aattaagaca atttagggca aagcagtcgg tagcgactgg tggcgcagga cctgtttcag
     1441 gaacacagcc tgcaccgcac ctcgcgtttg cggcctacgt ctaggatcac gagacccctt
     1501 ggaacgagaa tgccttgagg tcttcggcaa tcaacttcag ttggcgctgg agatcgtctc
     1561 gtgcttgacg caaatcgcca agggccgagg gcggtagata accgaaaaca tagaacttac
     1621 atttcggctc cgtgcctgaa ggccggatgg ccacgcgatt gtcgcccttt tcgtctaggt
     1681 gcaggagtac gacgtttcct cggcgggctg cggccatggc gtattcccac tcttggcttg
     1741 gatcaacgcc aatcggctga ttgagatcat gaacagagac aactctttgc ccgccaagct
     1801 ctttgggtgg gtttgtccgg aacttctgca ttaaatcctt cataagctgc atgccgcgct
     1861 cacccggcag gtcaatagag aaggccattt cctcgtggag tccatgctct cggaatagtt
     1921 cctccaatcg ccccacaagt gtctgaccgc gggcttttaa ttcggcggcc agttcgcaaa
     1981 ggaccatggc agcaaccgca ccatctttgt cgcgggcatg atcaccgatc aaaaaaccgt
     2041 aggactcttc ggccccaatc agaaaccgat cgggcccttc ataatccatc acgccgccga
     2101 tccatttgaa gcccaccatc agatcatcaa tgacacggac gccggccttt cgcgcaatcc
     2161 gccccagcaa ccgggtagtc accagcgtct ccacgacata gtgctgaggc gtcagctggc
     2221 cagct
//
LOCUS       DAOPTR010000695         2225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_38559_length_2225_cov_2.752074, whole genome shotgun sequence.
ACCESSION   DAOPTR010000695 DAOPTR010000000
VERSION     DAOPTR010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38559_length_2225_cov_2.752074"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2225
                     /locus_tag="PKI05_12260"
     CDS             <1..>2225
                     /locus_tag="PKI05_12260"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOA53017.1"
                     /translation="HYVDKATQIEYPDVEAESLDEVQKASPPLTLSNSDVKEYWDLSL
                     EEAIRIALQNSKIIRTLSQPDVGMPRLPETSLTVAPEGTPTVYDPARQETNPRTGVEG
                     VLADFDAHWSTRFFWQKSDTPQNVAGFVQAFRPQAFRQDTGNFETEISKTYATGGTFS
                     ISHSVGYDWNNAFTSRQWSSHWTANVEAGFRQPLLQGAGVTFNRIAGPNSIPGFYNGV
                     LIARLETDQRLAEFEQSVSELLLDVEKAYWTLYLSYRQLDTVIAGRDAALQTWRQVRA
                     KYEVGGRGGSAQEEAQSRQQYLAFRAAVEQALSSLYKAENNLRYLMGLTATDGRLIRP
                     KDEPTRARVEFDFHDVHAEALIRNPLLRREKWRVKKAELELIAAKNYLLPKLDMFGFY
                     RWNGLGHDLIDPDDDRSNAYGSLTSGDYQDWQLGMELQIPIGFRKELAAVRNAQLNLA
                     RERAILQEQELEISHQIAWVLREIDEYYTLAQTNYNRTVAAENEVRAVTAMYDAGTAT
                     LDLLLNAQQRRAEAEIQYYQSLTKYMFAISHLHFRKGSLLEYNGVYLTEGPWPQKAYF
                     DALRRARARDAGLYLNYGMTNPPVFSRGPYDLHAGKDGAEELLIDGLPDGVEINQQGA
                     PSVIELEPPPMPSEDMPGSTSDASKTEQAAEQKDASRQTRREDWTTSVPQQRRASTDD
                     GSSAAISRKQDQLVALASHVSYTSRTSSTPSAPSPSKNQPSEQGAGDAPSSPGQATHT
                     T"
BASE COUNT          539 a          602 c          640 g          444 t
ORIGIN      
        1 cccattatgt ggacaaggcc acacagatcg agtaccccga tgtagaggca gagtcgctgg
       61 atgaagtaca aaaggcttca ccgcccttga cgctatccaa cagcgatgtg aaagagtatt
      121 gggacttatc cctggaagag gccattcgga tcgccctgca gaatagcaag atcattcgca
      181 ctttgagcca accggacgtg ggaatgccgc gattgccgga gacatcattg accgtcgcgc
      241 ccgaagggac gcccacggtc tatgacccgg cacgccagga aacaaacccg cgaacagggg
      301 tggaaggtgt cctcgccgac ttcgatgccc attggagcac tcggtttttc tggcagaaat
      361 ctgatacacc ccaaaacgtg gccggatttg tccaagcgtt ccgcccccaa gccttccgtc
      421 aggataccgg gaatttcgag accgaaatct cgaagacgta cgcaacgggt gggacgtttt
      481 ccatttcgca ttctgtgggc tacgactgga ataatgcctt cacgagccgc cagtggtcaa
      541 gccattggac ggcgaatgtg gaggccggtt ttcggcaacc gttgcttcag ggggcggggg
      601 tgactttcaa ccggattgcc ggtcccaaca gtattccagg tttttataat ggcgtcctca
      661 ttgcgcggtt agaaaccgac cagaggcttg ccgagttcga gcaatctgtc tcggagttgc
      721 tcctcgacgt ggaaaaggcc tactggacgt tgtatctcag ctatcgccaa ttggataccg
      781 tgattgcggg acgcgatgcg gccttgcaga cgtggcgtca ggttcgggcc aaatacgagg
      841 taggtggacg cggcggaagt gcccaggagg aggcccagtc gcgtcaacaa tacctggcct
      901 tccgcgcagc cgtggagcaa gccctaagtt cactatacaa ggcggaaaat aacttgcggt
      961 atttgatggg gctgacagcg acggatgggc gtctcattcg gcccaaggac gaacctacgc
     1021 gggcaagagt ggagtttgac tttcacgacg ttcacgctga ggcactcatc cgcaatccgc
     1081 tcttgcggcg agaaaagtgg cgcgtcaaaa aggcggaatt ggagttgatt gctgcgaaga
     1141 attatctcct gccaaaactt gatatgtttg ggttctacag atggaacggc ctagggcacg
     1201 atttgatcga tccggatgat gatcgctcca acgcgtatgg gtcgctaaca tcgggggact
     1261 atcaggattg gcaattggga atggaactgc agattcccat tgggttccga aaagaattgg
     1321 ctgcggtgcg gaatgcccag ctcaatttgg cacgtgaacg cgctatcctc caagagcagg
     1381 aattggagat atctcaccag attgcctggg tgttgcgtga gatcgacgag tattacaccc
     1441 ttgcccagac caactacaac cgaacggtgg ctgcagaaaa cgaggtccgc gctgtcactg
     1501 ccatgtacga tgccggcacg gcaacattgg acttgttact gaatgcccaa cagcgtcggg
     1561 cggaagccga aattcagtac taccagtcat tgacgaaata catgtttgcc atcagccatt
     1621 tgcatttccg caaggggtcc ctgttagagt acaacggcgt ttacctcaca gaaggaccgt
     1681 ggccacaaaa ggcctatttc gatgccttgc ggcgggcacg agcaagagat gccgggctct
     1741 acttgaacta cggaatgacg aatccaccgg tcttcagccg tgggccgtat gatcttcatg
     1801 ctgggaagga tggggctgag gagctcctga tcgacggttt accggatggc gtggaaatca
     1861 atcagcaggg cgcgccttct gtgatcgagc ttgaaccccc acccatgccc agcgaagaca
     1921 tgccgggttc aacaagcgat gcttctaaaa ctgaacaagc tgcagaacag aaagatgcgt
     1981 cgcgacaaac aaggcgggag gattggacga cgagcgtccc ccaacaaagg cgcgccagca
     2041 ctgacgacgg aagttcggcg gcgataagcc ggaaacaaga ccagcttgtg gccttggcct
     2101 cgcacgtctc ctatacctcc cgaacttctt ccactccctc agcaccgtca ccatcaaaga
     2161 atcaacccag cgagcaaggt gcgggagacg ctccctcttc gccagggcaa gcgacgcaca
     2221 ccacc
//
LOCUS       DAOPTR010000696         2220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_38653_length_2220_cov_2.757968, whole genome shotgun sequence.
ACCESSION   DAOPTR010000696 DAOPTR010000000
VERSION     DAOPTR010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38653_length_2220_cov_2.757968"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PKI05_12265"
     CDS             <1..518
                     /locus_tag="PKI05_12265"
                     /inference="COORDINATES: protein motif:HMM:NF027702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA53018.1"
                     /translation="AVEHLGDHRPSGPFRGGKYSAFEGGTRVPMIVRWPNKIAAGTKS
                     DALVCHIDFLASFAALVGQSLPEDVAIDSVNVLPALLGKTQVGREELVEQAGGLALRQ
                     GNWKYIPPRKGPAINKNTNIELGVDPNGFLFNLENDPGEQNNLISSLPDKAKAMAERL
                     QAIRDAKQGGP"
     gene            773..1312
                     /locus_tag="PKI05_12270"
     CDS             773..1312
                     /locus_tag="PKI05_12270"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HOA53019.1"
                     /translation="MIDTTHRRNSPGIRPIRNGPARREGALTLELVLVLPMLLVMILA
                     VVQFGQYLANVQELALAARVGGEEAAISANLPTTEGAPVPSAVVTAIDQQLLAAGIVH
                     RMIVLEHNVGRHQVSLVTPPGADPPPKLASVPPGQYVRVLIIVPKSEVMPNLLKTLGL
                     NLVMPDSTTLVSYVMKHEG"
     gene            1437..>2220
                     /locus_tag="PKI05_12275"
     CDS             1437..>2220
                     /locus_tag="PKI05_12275"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HOA53020.1"
                     /translation="MSKHHTPMNRRGISALWFILTLPLCVILFGFVVELGHLWIARVE
                     LENALEAAARAAVDTWAQSGSGTTYPARQVGVAYAAANYVRGIPLQIGLNYEPSPSPD
                     NPNENMTCCVQPGDPLGGILSEGNLVFGTIIRDPNAQCPITFDTRKAPGCSPGTVLVD
                     ATAQGPGNLAQDNAWGIVFRYAPNLPSGLTIMRVIIDLQASGGSGRFDFTSSGPVLSD
                     SSPAPIIPCHYDVEGFTNPTTQISFTPTSGYSPTLTIDFFPDP"
BASE COUNT          491 a          611 c          584 g          534 t
ORIGIN      
        1 gtgccgtgga acatctggga gatcatcgac ctagtggtcc ctttcgcggg ggaaaataca
       61 gcgcgtttga gggtggaacg cgggttccca tgattgtccg ttggcccaac aaaattgctg
      121 ccggcacgaa aagtgatgcc ttggtatgcc acatcgattt tctcgcatcg tttgcggccc
      181 ttgtcggcca atccttacca gaggatgttg ccatcgacag cgtgaatgtg cttccagccc
      241 ttttgggtaa aacccaagtg ggtagggaag aacttgtcga acaagccggc ggattggcgc
      301 tgcgacaggg aaactggaag tacatcccac cgcgaaaggg acctgcaatt aataagaaca
      361 ccaatatcga gttaggtgtc gatccgaacg gctttttgtt caatctcgaa aacgacccgg
      421 gagagcaaaa caatctgatc tcgtcactcc cggataaggc caaggccatg gcagagcgtc
      481 tccaagcgat tcgtgacgcc aagcaaggcg gaccgtaaca atcagcgagc accattgcgg
      541 cgggtactca accaggtcca tgatatgttg ccaaagggca acgtgttgta aggataacgt
      601 tgcccatttg gaacgtttga cgtttgcttt tcttaacctg tcggtacgtc cacgattcct
      661 ttggtattgg aatcaatttt gctcgccagc agtatcgata aacagctttc ggtacacgtg
      721 cagccgctac acatacgcaa gcgtacctcc gaagcggtga gacctaaccc ccatgattga
      781 cacgacgcat cgccggaaca gcccaggtat taggcccatt cgaaacggcc ctgcccgtcg
      841 tgagggagct cttacgctcg agcttgtgct ggtgctgccg atgctgctcg tcatgatcct
      901 tgcggttgtg cagtttggcc agtacctggc gaacgttcag gagttggccc ttgctgcccg
      961 cgttggcgga gaagaggcag ctatttccgc gaatctaccc acaaccgagg gtgcaccagt
     1021 cccatctgcc gtggtcactg cgatcgatca acagctccta gctgccggga ttgtgcaccg
     1081 gatgattgtc ctcgaacaca acgtgggacg ccaccaggtc agtttggtca cgcctcccgg
     1141 ggctgatcct cccccgaaat tggcctcggt tccgccgggc caatatgtcc gtgtgctcat
     1201 tattgtgccc aagagtgaag tcatgccaaa tctccttaag actctcgggc ttaatctggt
     1261 tatgccggat agtacaacac ttgtctcgta cgttatgaag cacgaggggt aacacgaacg
     1321 ccaggtccag tccttgtcct tgtctgccga gtactcaaag gaaccatagt cgcgtcgtct
     1381 ggttcctccc agaggacgca tactactgtg gttatttact gttcaatagg ttcacaatga
     1441 gcaaacatca cactcctatg aaccgccgag gtatctcagc gttatggttc atcctgacgc
     1501 ttcctctttg cgtgatcttg tttggatttg ttgtagagtt gggacaccta tggatcgcac
     1561 gcgttgaatt ggaaaacgcg ctcgaggccg ctgccagggc tgccgttgat acgtgggcac
     1621 aatccggcag tggaacgacc tacccggctc ggcaagtggg tgttgcttac gctgcggcaa
     1681 attatgttcg tggtattcct ctccagattg gcttgaacta cgaaccctca ccaagtccgg
     1741 ataaccccaa cgagaatatg acgtgttgtg ttcagccggg cgatcccctc ggtgggattc
     1801 tttctgaagg gaatcttgtg tttggaacca ttatccgaga tcccaacgca caatgcccca
     1861 tcacattcga tacgagaaag gccccaggat gtagcccggg tacagtgctc gttgatgcca
     1921 cggcccaggg gcctgggaac ttggctcagg ataacgcttg gggaatcgtc tttcgttacg
     1981 ctccaaacct gccaagtggt ttgaccatca tgcgggttat cattgatctc caagccagtg
     2041 gtggatcggg acggtttgat tttacatcga gtggccccgt cctttctgac agtagccctg
     2101 ctccgatcat cccctgccac tatgacgtgg aaggttttac caatccgaca acccaaatta
     2161 gtttcacacc cacgtccggc tactcaccta ctctgacgat tgacttcttt cccgatccca
//
LOCUS       DAOPTR010000697         2217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_38728_length_2217_cov_2.752081, whole genome shotgun sequence.
ACCESSION   DAOPTR010000697 DAOPTR010000000
VERSION     DAOPTR010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38728_length_2217_cov_2.752081"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PKI05_12280"
     CDS             <1..218
                     /locus_tag="PKI05_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53021.1"
                     /translation="LTTSQLVPTAVLGILLSFTMLSGLLTNVTLLPWLLLITPRNKNC
                     SNEGTMPASNDRKTQNEIVMDKLEGDS"
     gene            241..1227
                     /locus_tag="PKI05_12285"
     CDS             241..1227
                     /locus_tag="PKI05_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53022.1"
                     /translation="MRLMHIGTDEAGYGPTLGPLVITATVWEGPHHLYPNHWYDLLSP
                     FVVSAPRLRPSGTGNRDDEQAPVVITDSKRIYRSGGGLGGLERTALGLLGLAHGIPKS
                     WQELIEYVDPAALADLSITPWLADFNPPLPVAATPNQVERATNQLLQAMDKADLRCTK
                     IRCRFITAFRFNQELERFASKSELLSHVTLRIVCELLPNQGEVILWADRHGGRAHYAN
                     VLYQAFDASFVHIEQESTDRSSYRVSRAQHQFRAVFLTKAERLLPVAAASLVSKYLRE
                     LAMLAFNRFWQRRVPGLRPTAGYPVDAQRFLAEIASIQQRLGLSADLVRRRK"
     gene            complement(1260..>2217)
                     /locus_tag="PKI05_12290"
     CDS             complement(1260..>2217)
                     /locus_tag="PKI05_12290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03009 domain-containing protein"
                     /protein_id="HOA53023.1"
                     /translation="MAQGIPQGFQAQTPTGNAAPGYPNYRSQPGAQPVPGNTVSPGAV
                     VAGQSVPPQGGNNVVAPPFVLTPAEQEQLDRVLLAWEERSSKIKTFECEFTRWEFDPV
                     WGPPNQPKRIVRGKIMYSAPDKGLFRVDEEVINGRLQPAAQPERWVCDGRSIYEYRFE
                     TKQIVETPLPPELQGKTITEGPLPFLFGAQAESLKRRYYMRIITPQNVTEEIWLEAFP
                     KYQQEAANFQRAQLILKLPQLDIFALQLYHPSAQAASQSRTVFQFDRIKINAVDFLRL
                     FKEDPFKPVLPDSSWAKVIETSKPGQQPESAMGQRPTPYRAQ"
BASE COUNT          465 a          632 c          590 g          530 t
ORIGIN      
        1 gtttgacgac cagtcaactt gtgccaaccg cggtcttggg aatccttctg agttttacca
       61 tgctcagtgg cctgctcaca aacgtaacgc tcttgccgtg gcttctgttg atcactccgc
      121 gaaacaagaa ctgttctaac gaaggcacca tgcctgcttc gaatgataga aagacacaaa
      181 atgagatagt aatggacaag ttagaaggcg attcgtaatc gccctttttg gagagacatt
      241 atgcgtctga tgcatattgg aacagatgag gccggttacg gccccacgct tggccccctt
      301 gtcatcacgg ccacggtatg ggaaggacct caccatcttt accccaatca ctggtatgac
      361 ctgctttcgc ctttcgtggt cagtgcccct cggcttcggc ccagtggaac cggcaataga
      421 gatgacgagc aagcccccgt cgttatcacg gattccaaac gaatctaccg ctctggtggc
      481 ggtttgggcg gtcttgaacg cacggctttg ggtctccttg ggttggccca tggcattcca
      541 aaaagctggc aggagcttat tgagtatgtt gaccctgccg cgcttgcgga tctttcgata
      601 acgccctggt tagcagattt caacccgccg ctacccgtgg ctgcaacccc caaccaggtc
      661 gagagggcca caaaccagct tttgcaagcc atggacaaag ccgaccttcg gtgcacgaag
      721 attcgctgcc gatttatcac ggcctttcga tttaatcagg aactggaacg cttcgcgtca
      781 aaatcggagt tgctttccca tgtgaccctt cggattgtgt gtgaactcct tcccaaccaa
      841 ggggaggtca tcctttgggc agatcgacac ggcggacggg cccattacgc caacgttctg
      901 tatcaggcct tcgacgccag ctttgttcat atcgagcaag aatccacaga tcgaagctcc
      961 tatcgagtgt cccgcgccca acaccagttc agggctgtct ttctcacgaa ggccgagcgg
     1021 ctcttgccgg ttgccgcggc ctccctcgtc tcaaaatacc tgcgagaact ggctatgctg
     1081 gccttcaatc gtttttggca gcgtcgcgtt cctggccttc ggcccacggc gggctatccc
     1141 gtggatgcgc agcgtttctt agccgagatt gcctctattc agcaaagact aggtctttct
     1201 gcggacctcg tccgccgccg caaatagccg tacccaatcc cgaacaaaca ccggcttcat
     1261 cattgcgccc gatacggagt tggtcgttgc cccatggcag attctggctg ctggccgggt
     1321 ttactcgttt cgatcacctt ggcccaggat gaatctggca acacaggttt gaagggatct
     1381 tccttaaaga ggcgcaagaa atcgacggcg ttgattttaa tgcggtcaaa ttgaaacacc
     1441 gttcgcgatt ggctggcagc ttgggcactg gggtggtaaa gctggagggc gaaaatatcg
     1501 agttgtggaa gtttcaagat caattgtgct cgctgaaagt tggccgcctc ctgctgatac
     1561 ttaggaaagg cttccaacca aatctcttcc gtgacgttct gcggcgtgat gatccgcatg
     1621 taataccgcc gcttgagtga ctccgcttgc gccccaaaaa gaaacggcaa gggtccttcg
     1681 gtgatcgtct ttccctgcaa ctctggcggg agtggtgttt cgacgatttg ctttgtctca
     1741 aatcggtact catatataga tcggccatcg cacacccaac gctcaggttg tgcggcgggt
     1801 tgcagtcgac cattgataac ctcctcatcc acgcggaata gccccttgtc cggtgcggag
     1861 tacatgatct taccccgcac gatacgtttc ggttgattgg gaggtcccca cacgggatca
     1921 aactcccagc gggtgaattc acattcgaag gtcttgattt tggaactgcg ctcctcccag
     1981 gccagcaaga cgcgatcgag ctgctcttgc tcagcaggag taagcacaaa agggggggca
     2041 acgacgttat tgccgccttg tggcggaacg ctctgccccg ccacaacagc cccgggcgaa
     2101 actgtattgc ccggcaccgg ctgcgctcct ggttgagatc gatagtttgg gtaacctgga
     2161 gccgcatttc ccgtcggtgt ttgtgcctga aatccctgcg gaatcccctg cgccatg
//
LOCUS       DAOPTR010000698         2216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_38748_length_2216_cov_2.814438, whole genome shotgun sequence.
ACCESSION   DAOPTR010000698 DAOPTR010000000
VERSION     DAOPTR010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38748_length_2216_cov_2.814438"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="PKI05_12295"
     CDS             complement(<1..1102)
                     /locus_tag="PKI05_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008667228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA53024.1"
                     /translation="MNTKVVLAVAKRNFISYFASPTAYLFICVFVALSTMAAFWPNEF
                     FDANLANLDQLNKWFPYVMLIFIPAITMGIWADERRQGTDELLLTMPATDWEVVLGKY
                     LAAMAIFTVSLAFSLICNFLVLRTLGSPDIGLFIATYIGYWLVGLAMLGIGMVASFFT
                     ANLTVAYILGVILNAPAVFLASADVIVRQDLAIRAKAWSVAEQFANFGRGLITLSGVA
                     YFLLIVAVALYVAMVLIGRRHWPTGESRLPSGLFFGLLHLAWIASFLLLGAIIGAKWG
                     NSSLLVVLLAIAYFILHAALIWLWTRFPNRGQPSLLHFIPPAAHIATVIIAAAVAVLW
                     GEVLGSGWVFWLLLAVLLALHLLFLLGWWQFPR"
     gene            complement(1223..1966)
                     /locus_tag="PKI05_12300"
     CDS             complement(1223..1966)
                     /locus_tag="PKI05_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA53025.1"
                     /translation="MEKSGRLMIEAVGLSKYYGDFAAIEDVSFEIREGEVVAFLGPNG
                     AGKSTTMKLLTGYLAPSAGTARIAGYDVFTQRLEASRHLGYLPENGPLYADMTPIGLL
                     LFFAEARGIPPERRRQRIHELVELCALESVVGKPIGKLSRGYRQRVGMAQVLLHEPDV
                     LIMDEPTAGLDPNQIHEVRETIRRLMAQNKTILLSTHILQEVEAMATRILFINDGRLV
                     FDGPVDEIVRSGKPMDEWFRELSGATAAV"
BASE COUNT          578 a          656 c          569 g          413 t
ORIGIN      
        1 cccggggaaa ttgccaccaa ccgagcaaaa acaggagatg gagcgccaat aggacagcca
       61 gtagcaacca gaagacccat cctgagccga gaacctcacc ccatagaacg gcaaccgctg
      121 ccgcgataat gaccgtcgcg atatgggccg ccggggggat aaaatgcaaa agcgacggtt
      181 gcccgcgatt gggaaaccgg gtccacaacc agatgagcgc cgcatgaagg ataaagtagg
      241 caatcgcaag caatacaacg agcagcgagg aatttcccca tttggcccca atgatcgctc
      301 ctagaaggag aaagcttgct atccacgcca gatggagcaa tccgaaaaac agtcccgaag
      361 gaagccgtga ttcccccgtg ggccagtgtc gccgtccaat caacaccatc gccacataga
      421 gggccaccgc cacaatcagc agaaaatacg ccacgccgga gagtgttatc aaacctcgtc
      481 cgaagttggc aaactgttcc gcgacactcc aggcttttgc ccgaatcgcc aagtcctgcc
      541 gcacaatgac gtccgccgaa gccaagaaga ccgcgggcgc gttaagaatc acgcccaaga
      601 tataggccac cgtcaaattg gccgtgaaaa acgacgccac catgccgatg cccaacatgg
      661 ccaggcccac cagccagtat ccaatatagg tggcaataaa aagcccaata tcgggtgaac
      721 ccaatgtgcg gaggacgagg aaattgcaaa tcaaggaaaa ggcaagagaa accgtgaaaa
      781 tcgccatcgc cgccaggtat ttaccgagaa ccacttccca atcggtcgcg ggcatggtta
      841 agaggagttc gtcggttcct tggcgtcgct cgtcggccca aatacccatg gtgatggccg
      901 gaatgaaaat cagcatcacg taagggaacc acttattgag ctgatctaag ttggcgaggt
      961 tggcatcgaa gaactcattg ggccaaaagg ccgccatggt actgagggcg acaaacacgc
     1021 agataaacag gtaagccgtg gggcttgcaa aataactgat gaagttccgt ttcgcaaccg
     1081 ccaagactac ctttgtattc atgggtatat cacctctttg ccgttatcca cagagcctct
     1141 accgaaccat caccctgttg ccacgaggaa agaaaaccaa ttggatcgac caggtctgag
     1201 acgtggctac acttcaacca cctcagacag ccgccgtggc acccgagagc tcacggaacc
     1261 actcatccat gggcttaccg cttcgcacaa tctcgtcaac aggcccgtcg aagaccaaac
     1321 gaccgtcatt gatgaacaaa atccgcgtcg ccatggcctc gacctcttgg agaatatgcg
     1381 tggaaagaag aatggtcttg ttctgggcca tcaggcgacg aatcgtctcg cggacttcat
     1441 gaatctgatt gggatcgaga ccggccgtgg gttcgtccat gatcaggacg tcgggctcgt
     1501 gaagcaacac ctgagccatg cccacccgct gccgataacc tcgggagagc tttccgatgg
     1561 gtttgccaac gacactctcc aaggcacaca actccaccag ctcgtggatt cgctggcgcc
     1621 gccgctcggg gggaattccc ctggcctctg cgaaaaagag gagcaatcca atgggcgtca
     1681 tgtcggcgta aagcggacca ttttcaggaa gatagcccaa atgccgcgaa gcctccaatc
     1741 gttgtgtgaa tacatcgtag ccggcaatgc gggctgtccc agcactgggc gccaggtaac
     1801 ccgtcagtag cttcatcgtc gtgctcttcc ctgccccgtt tgggccgaga aaggcgacga
     1861 cttctccttc tcgaatctcg aatgagacat cctcgatcgc cgcgaaatcg ccataatact
     1921 tggaaagtcc cacggcctcg atcatcaagc gaccactttt ttccatagct gactcctcgc
     1981 ggaacttgtt cggatatctc ttctcgtcct cgctggtttc ccgcatgttc gctcagccca
     2041 gcggtagaaa cgcctgtcta agatgtcatg gtgctgcaag gttgtgcaaa ttacctgatc
     2101 cttgttccca cagttcctcc ctctaacggg tcggggcggc aaaccgctgc gtgctacgct
     2161 gaacccacga agatagttca tgctgcacgc ctagcacgca gtccaaatgc gtcatc
//
LOCUS       DAOPTR010000699         2214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_38800_length_2214_cov_2.897638, whole genome shotgun sequence.
ACCESSION   DAOPTR010000699 DAOPTR010000000
VERSION     DAOPTR010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38800_length_2214_cov_2.897638"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1408)
                     /locus_tag="PKI05_12305"
     CDS             complement(<1..1408)
                     /locus_tag="PKI05_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53026.1"
                     /translation="MIPGRDGCASGAASAKTDGKFGIGKESPTLSTKDVAPIRIIWRW
                     VQRTVGVVPLGERCDRAAALAFVLSMTWNGLWLLAIFVTAFATGRVFAEDVSFPLPNR
                     SFPIDIKAAAGNHWRMGSYEVWILRGWCEIRQGSVSLRARESVIWIDRQAEPSVGLGD
                     PVTPGVAAPALEEGPLLLAPIPDETSDRSGSAFALPRHASGTGRLIKVIIYLEGRAQV
                     VSDDPQHRLQIQDKTWFGRLYTTADVNVQVKEEAGEPEPYPAIFARAMARRNAATEGL
                     TQSVLRPNEFLSDPSPRGASEGAMRRIRVFPRGDVPIQAQWFPAPQGGQWIGVIDAGI
                     NLIVEEPGPWGTLDVSADRVVIWYTGDEAPNLSGEATTPQQWPLELYLEGNIVFRQGD
                     RIIYADRMYYDVARRAGMILNAELLTRIPDYEGYLRLRADILQQMNETQFFAQNLFVT
                     SSRMGVPGYRLQTQFAYVE"
     gene            1913..>2214
                     /locus_tag="PKI05_12310"
     CDS             1913..>2214
                     /locus_tag="PKI05_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53027.1"
                     /translation="MDLKRVTLPEKELPSEWYNLLADLPQPLPPPVHPVTKEPLDPAA
                     LERIFARELIKQEVSQERWIGIPDEVRRIYAIWRPSPLVRARNLEKALKTPARIYF"
BASE COUNT          444 a          646 c          668 g          456 t
ORIGIN      
        1 cctccacgta ggcgaactgt gtttgcagtc gataaccggg aacgcccatc cggctcgatg
       61 tgacgaagag attttgggcg aagaattgcg tctcgttcat ttgttggagg atgtcggcac
      121 gaagccggag gtagccctcg taatcgggaa tccgtgtgag caattcggcg ttgaggatca
      181 tccctgcccg ccgcgccaca tcgtaataca tgcgatcggc atagatgatg cgatctccct
      241 ggcgaaagac gatgttgccc tcgaggtaca gttccagagg ccactgctgg ggtgtggtgg
      301 cctcaccgct gaggttgggg gcctcgtcgc ccgtgtacca aatgacgact cggtcggctg
      361 agacgtcaag ggttccccag gggcctggct cttcgacaat gagattgatc ccggcgtcaa
      421 tcacgccgat ccattggcca ccctgtgggg ccggaaacca ttgggcctgg atggggacat
      481 caccgcgggg gaagacccga atccgtcgca tggcaccttc cgaagcacct cgcggcgaag
      541 gatcagaaag gaattcattc ggtcggagga cgctttgcgt cagcccttcc gtggcagcgt
      601 tgcgccgcgc catcgcccga gcaaagatgg ccggataagg ctcgggttca ccggcctctt
      661 ctttgacctg aacgttgaca tcggcggtgg tgtacaaccg gccaaaccag gtcttgtcct
      721 ggatttgtag ccgatgttgg ggatcgtccg agaccacttg ggcacggccc tccaaataga
      781 tgatgacctt tatcaggcga ccggtccccg atgcatgacg cggcagggcg aatgccgatc
      841 cggatcgatc agacgtctcg tcagggattg gggccagaag gagtggccct tcttccaagg
      901 ccggggccgc tacgccaggt gtgaccggat ctccgaggcc caccgacggt tcggcctgcc
      961 gatcgatcca gattacggac tcccgcgcgc ggaggcttac cgatccctgc cgaatctcgc
     1021 accacccgcg gagaatccac acctcgtagc tgcccatccg ccagtggttg cccgccgcgg
     1081 ccttgatgtc aatagggaaa ctcctattcg gcagcgggaa cgagacatcc tccgcgaaga
     1141 cccttccagt ggcaaaagcg gtcacgaaaa tcgctagcag ccacaggccg ttccacgtca
     1201 tcgacaggac gaacgccagt gccgccgcgc gatcgcagcg ctccccaagg ggtacgaccc
     1261 ctacggtccg ctggacccat ctccagatga tccgaattgg tgcaacgtcc ttagtgctca
     1321 gagttggcga ttccttgccg atgccgaatt taccgtcagt ctttgccgag gccgcacccg
     1381 aggcacaccc atcgcggcct ggaatcacga gctcaccccc actgaggtag ggattgcgga
     1441 ggggagatta tagtggaaca agtccaatga cgacaaggca acttctttga gccttagagc
     1501 ctcccccata atctcctctc actagttcgg cgggagaggg tccgggggcg agggtggctc
     1561 gaatcgctgc cggttttctc aatcgattcc gatgttcgct tgccctcacc ccaaccctct
     1621 cccggaggga gagggggctt tttgggatag actcttggca gacccctgcc gacggcttgt
     1681 cggttgcttg aggtgcggac accgcagctt atccatttcg cggaatcttc ttttcggcag
     1741 gcggggaccc ggtcgagggc aacgtttttt gcttcactac cagaaatttt gaggaaatca
     1801 tggtataaat ggtgttttca gtgtttggga cgggtttggc gcggatcaac atgtcgaatg
     1861 aacggccgac ggccgagact gacaagatag gtcgttgagg aaggaacatt ctatggactt
     1921 gaaacgtgtg acattgcccg agaaagaact tccttcggaa tggtacaacc tgctggccga
     1981 tttgccccag ccactgcctc cgccggttca tccggtgacg aaagagccgc ttgatccggc
     2041 tgccctggag cggatttttg cccgggaatt gatcaagcaa gaggttagcc aggagcggtg
     2101 gatcgggatt cccgacgagg tccggcggat ttatgccatt tggcggccct cgccccttgt
     2161 ccgtgcgcgg aatttggaga aggcccttaa gaccccggca cgcatctact ttaa
//
LOCUS       DAOPTR010000700         2209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_38918_length_2209_cov_3.426184, whole genome shotgun sequence.
ACCESSION   DAOPTR010000700 DAOPTR010000000
VERSION     DAOPTR010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38918_length_2209_cov_3.426184"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2209
                     /locus_tag="PKI05_12315"
     CDS             <1..>2209
                     /locus_tag="PKI05_12315"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOA53028.1"
                     /translation="SEERPITYRELDRQARAIAAWLQTHDLVGERALLLYPPGLEFIA
                     AFFGCLYGGVVAVPVYPPRRNRSMERIEAISDNARAKVALTTRAVRERVDSLIHETPH
                     LQQLTWLTTSEIEPGMEDRWQVPDISGETLAFLQYTSGSTGTPKGVMLTHANLMHNSA
                     LICYAFEHRRSGTGVYWLPSYHDMGLIGGIIQPVFVGRPNVMMSPMAFLQKPYRWLAA
                     ITKYRGTTSGGPNFAYDLCVRSITPEQRATLDLSSWEVAFNGAEPVRAETIERFSEYF
                     APCGFRKEAFYPCYGLAESTLMVSGGFVKEPPVIRFFDPEEIARGRAVICGPDNPRAR
                     PLVGSGRQLPDGRIVIADPETHRRLPDGQIGEIWVQSPSVAIGYWENEQATRETFHAH
                     LADGDGPFMRTGDLGFLWEGELFVTGRIKDMIIVHGVNVYPHDIEQTVQGADPRLRQN
                     AGACFAVEQDGREQLVIVQEVERRVKDGFDAIFQAIRRAVSLEHELAVDAIVLIKPGT
                     IPRTSSNKIQRFACRQAYLEGTLEVVASWKRGDTPDEWKEPALTASSVRQTGETGRIS
                     DVGTSGHSPDNGDDHGPLAMDQLAARLATVSQTGPAWQAAGKRAGPNGEDSPDRMTKA
                     ASLASASGAAKPPLDRQQIVQAVLSEVRRVARDRAEGLTLDTAITEIGLDSLERMEIL
                     ASLEERFGGRFPEEILPELETCAQVVEAVEKYLGGQEQERRVVIPPDQEIPAEF"
BASE COUNT          457 a          639 c          666 g          447 t
ORIGIN      
        1 aagcgaggag cgaccgatta cctatcgcga gttagaccga caggcacgag ccattgccgc
       61 ctggcttcaa actcacgact tagtgggcga acgcgcctta ttactttatc cccctggcct
      121 cgagttcatc gccgcgttct tcggttgcct gtacggtggt gtggttgccg tgccagtcta
      181 tcctccgcgg cgaaaccgct ccatggagcg aatcgaagcg atctcggaca acgcgcgggc
      241 taaggtggcc ttgacgacac gggcggtccg cgagcgggtt gacagcttaa tccacgaaac
      301 gccccacctt cagcaattga cctggctgac cacgagcgag atcgagccgg ggatggaaga
      361 ccgctggcag gtgcccgata tttcgggcga gacgctcgcc tttttgcaat acacctcggg
      421 atcaacggga accccgaagg gcgttatgct gacgcacgcc aaccttatgc acaactcggc
      481 cctcatctgt tacgcctttg aacatcgacg atcaggaacg ggcgtttact ggctccccag
      541 ttatcacgac atgggtctca ttggcgggat catccagccc gttttcgtgg gacgccccaa
      601 cgtcatgatg tctcccatgg ccttcttgca gaagccctat cgttggttgg ccgccattac
      661 caagtaccgg ggaaccacca gtggaggccc gaattttgct tatgatttat gcgtgcgatc
      721 catcacgccg gagcaacgag cgactctcga tcttagctct tgggaagttg ccttcaatgg
      781 ggccgagccg gttcgggcag agacaatcga aaggttcagc gaatacttcg ccccgtgtgg
      841 cttccggaag gaggccttct acccgtgcta tggtttggcc gaatccacgt tgatggtctc
      901 cggtggattc gtgaaggaac ctcccgtgat tcgcttcttt gaccccgagg agattgcccg
      961 ggggcgtgcc gtgatctgtg gccccgacaa cccccgcgct cgcccgttgg taggatcggg
     1021 acgacaactc ccggatgggc gaatcgtcat tgccgatccg gagacgcatc gtcggctgcc
     1081 agatgggcag atcggcgaaa tctgggtgca aagtccttct gttgccattg gctattggga
     1141 aaatgagcaa gccacgcgag aaaccttcca cgcccatttg gccgatggcg acggaccgtt
     1201 catgcgaaca ggcgatttgg gattcctttg ggagggagag cttttcgtca ccggccgcat
     1261 caaggatatg atcatcgtcc acggggtgaa cgtttatccc cacgacattg agcagacggt
     1321 ccagggggct gatccgcgat tgcggcaaaa tgccggtgcc tgctttgccg ttgagcaaga
     1381 cggccgggag caactcgtca tcgtccaaga ggtcgaacgc cgagtcaaag atggatttga
     1441 cgccatcttc caggccatca ggcgagccgt ctccctggaa catgagttgg cggtcgatgc
     1501 gatcgtgctg atcaaacccg gtaccatccc ccgcacgtcc agcaacaaga ttcagcgatt
     1561 cgcctgccgg caggcctatc tggaagggac gctcgaggtc gtcgcaagtt ggaagcgcgg
     1621 tgacacacct gatgaatgga aagagcctgc gctaacggct agctcggttc gccaaaccgg
     1681 agaaactggc aggatatccg atgttggcac ctcaggacat tcgccggaca acggggacga
     1741 tcacggtccc ttggcgatgg accaattggc ggcacgcttg gccacggtga gtcaaacggg
     1801 cccggcctgg caggccgccg gtaaacgagc agggccgaac ggcgaagact cgcccgatcg
     1861 aatgaccaag gcggcttcgc ttgcatcagc gtcaggcgcg gcgaaaccac ctctcgatcg
     1921 gcagcagatc gtgcaagcgg ttctttccga ggttcgtcgt gttgctcgcg accgtgccga
     1981 aggcttgacc cttgacacgg ccatcacgga aataggtctc gattcgctgg aacgcatgga
     2041 gattttggcg tccctggaag aacgattcgg tgggcgattt cccgaagaaa tcctccctga
     2101 gctggaaacc tgcgcccaag ttgtcgaggc ggtggaaaag tatctcggtg gtcaggagca
     2161 ggaacgccgt gttgtgattc ctcccgacca agagattcca gcagagttt
//
LOCUS       DAOPTR010000701         2208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_38936_length_2208_cov_3.141198, whole genome shotgun sequence.
ACCESSION   DAOPTR010000701 DAOPTR010000000
VERSION     DAOPTR010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38936_length_2208_cov_3.141198"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1018)
                     /locus_tag="PKI05_12320"
     CDS             complement(170..1018)
                     /locus_tag="PKI05_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOA53029.1"
                     /translation="MKKTKRRNPEENGVNSPLEAYLREINETPLLTPEEEMDLAIAIG
                     QGDPVARDRMVRANLRLVVNIARNYVGKGLSLQDLIEEGNLGLLRAVEGYDPAVGTRF
                     STYASYWIKQSIKRALINCGKTVRIPAYMVEILSKWRRASTRLAEELGRTPTPEEVAR
                     VLGIPRKKLSIIKKAIRIYNLAPQTDQAENGWSLGEMLEDERFQSPDEQLVTHDSLQH
                     IREKLEKMDPREAAVLRMRFGLDDRQPRTLKEIGNILGLTRERVRQIEAEALSKLAQA
                     LNAGEP"
     gene            1839..1926
                     /locus_tag="PKI05_12325"
     tRNA            1839..1926
                     /locus_tag="PKI05_12325"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1873..1875,aa:Ser,seq:gct)
BASE COUNT          456 a          643 c          608 g          501 t
ORIGIN      
        1 agcctatccc aaaattccct ctccctctgg gagagggtta gggtgagggt aagccatcgt
       61 cggggtcgat cgagaaaacc agcagcgatt cgagccaccc tcacccccgg cccctctccc
      121 gccgaacggg cgaggagagg ttgcgggaca ggctcttacg caacaggtat taaggctccc
      181 ctgcattcag ggcctgggcc aacttgctca gggcctccgc ctcgatctgg cggacccgct
      241 ccctcgtcaa tcccagaatg ttgccaatct ccttgagtgt tctcggctga cgatcatcca
      301 acccaaagcg catccgtaag acggcggcct cgcgtgggtc catcttttcc agcttctccc
      361 gaatgtgttg gaggctatcg tgcgtcacaa gctgctcgtc cggtgactga aaacgctcat
      421 cctccagcat ctcgcccaaa gaccaaccgt tttccgcctg gtcggtttgc ggcgcaagat
      481 tgtagatacg gatcgccttc ttgatgatgg aaagcttttt gcggggaatg cctaagacgc
      541 gggcaacttc ctccggagtc ggcgtgcgac caagttcctc tgccagacgc gtactagcgc
      601 gtcgccactt ggaaagaatc tccaccatgt acgccgggat gcggaccgtc ttgccacagt
      661 tgatcagggc ccgcttgatc gactgcttga tccagtagct tgcgtaggtg gagaaacgtg
      721 tccccacggc ggggtcgtaa ccctcaacgg cccgcagtaa ccccaggtta ccctcttcaa
      781 tcaggtcctg aaggctgagg cccttgccta cataattccg cgcaatattc actactagac
      841 gtaaattcgc ccgaaccatg cgatcccgag caacggggtc gccttgcccg atggcaatgg
      901 cgagatccat ctcctcttct ggtgtgagaa ggggtgtctc attgatctcc cgtaggtatg
      961 cctcaagtgg agagttcacc ccgttctcct cagggtttcg tcgttttgtc ttcttcatag
     1021 ctgccgacac attttgtgca atcgcgacaa cagactttct gacccaatca agctatcggt
     1081 gaaggcaaca agcatcctgc aatgttagag aaaagcaaca agctgcacgg actgagactg
     1141 ttatcgcaat tttgtcaacg acgcatctcg caccagtcgt gcagaatgaa tcgacgaggc
     1201 cgctgggctc ggagggaatc cgcgctctct gaccgaacca cacgtgatga gtccccatcg
     1261 tgcggtgttg gtccttttga accgattatg cgtttcctac ccctttgtag cggctggcgc
     1321 ccatgcctac gcggcgaggt agatgaccac gcgctatctg gactgctatc tggagtaccg
     1381 agacacggaa agttgtgcac cgtcaccctt acgagaacgt aaagggctac gcccccggtg
     1441 gacgagacac ccaacggggt atcaatcgcc cgctggtaaa ccgctggtaa acgctgcgat
     1501 aacctccggc catcgtcatt gtcctttgag acatctactt tgcgcacttg gggcccatcc
     1561 agaaagtctc cctctccctt tgggagaggg ttggggtgag ggcaagcgat cgtcggaatc
     1621 gattgagaaa accgagagcg atacgagcca ccctcacccc cgactcctct cccgccgaat
     1681 gggcgacgag agattatggg aaaggctttt agaaggcaca tgacccccac acgagccatt
     1741 ccccatcggg ccttgtcaga cccgctaagc ttggtaaact tgaaatgagc gatggccttt
     1801 taggcctatc gcatgtaaag atggcgtatt tttgtgttgg agaggtggcc gagtggtcta
     1861 aggcgcgggt ttgctaaatc cgtaggggtg tccaacccct cgcaggttcg aatcctgtcc
     1921 tctccgatcc taagttatcg tcaaataatg acttacgtcg caagttgtgg caaggattga
     1981 agttcgggcg aggggtgttt tcgccccttt cctgtcgctt cgcttcattg cggcaatcca
     2041 ctgcagcagt atgtgcagca gtaacgggcg tccagtttcg ggctgcttcg tagtggcttt
     2101 cggtcgtttg ccagtagtgg gcctcggcaa tcgcttcggt atggccaagc cacgtcgcaa
     2161 cgacgtggga gggaaaactt gccgttaggt cggtggctag gctggccc
//
LOCUS       DAOPTR010000702         2205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39009_length_2205_cov_3.154884, whole genome shotgun sequence.
ACCESSION   DAOPTR010000702 DAOPTR010000000
VERSION     DAOPTR010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39009_length_2205_cov_3.154884"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="PKI05_12330"
     CDS             complement(<1..1051)
                     /locus_tag="PKI05_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53030.1"
                     /translation="MRLHVTIVFGVCLALAFHGVRTVSAEEWTTVKPKEIDAILPNPH
                     MGWETFHRTADRDQNLPEWIPSTVQYARWGWGRLEPRPGEIDYEFLDQQLAESRRAGQ
                     RLAFRVMCCSTTPKRPYHPDWLKAIGGRVITTRYGDTEGLEVPDLDDPVVLRAHLDFI
                     RRLGARYDGHPDLDHVDLGSVGWWGEWHMSRSDPAKMPTPENQKAIIDAYLASFKKTP
                     LLMLIDGPEMLRYATERGTGWRADCLGDMGGFSANWCHMRNAYPQTLTKANALDVWKR
                     APVAWETCWDIRKWVDEGWSLRYIFNYALALHASYINNKSAPLPPGDEVKAEVERFLR
                     RLGYRLVLRSLSHPRE"
     gene            complement(1109..>2205)
                     /locus_tag="PKI05_12335"
     CDS             complement(1109..>2205)
                     /locus_tag="PKI05_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53031.1"
                     /translation="MDWCEEHGVKGKYSVVPYPACVGWLDRDLPGWSHQELVESLRLV
                     RERIMPRWDIHPEMVTHTWVINTKTGRPYEERSARFMENWGWSEGRSVDELTDYLAYA
                     LRILKNVDLHCEGITTPGGFGSRARPQLAQATLEACRDVFRTEIPHYFRDLFTDERSV
                     APRVEYASGLDGDDPKCVVSIIGCTGDWFGGWDGTTEGSADLFITPDLAKGRMVDVIG
                     REEPAIMVCHWPGIYFNGEERGFEILKTVVQRLHARYENLIWMNLAEIARYWAARELT
                     RIEKNDTKVVFSAPFSAPLFTIQLTPLAEGVPAVGRAGEEQQRLRRVGRLLDLTSGTY
                     CEGSGTNGTEWLMTLCFDLPKGTSELVWLG"
BASE COUNT          516 a          682 c          532 g          475 t
ORIGIN      
        1 tctcacgcgg atgagacagc gaccgcaaca cgaggcgata acccaatcga cggagaaatc
       61 gttcgacctc ggccttaact tcatcgcccg gcgggagggg ggctgatttg ttattgatgt
      121 acgaggcatg aagggccagg gcgtaattga aaatgtaccg caacgaccac ccttcgtcga
      181 cccattttcg aatatcccaa catgtttccc aagccaccgg cgctcgcttc catacatcga
      241 gcgcattcgc cttggtcagt gtctgtgggt atgcgtttcg catgtgacac caattggcag
      301 aaaaaccacc catgtcgcca aggcaatccg cccgccatcc cgtccctcgc tcggtcgcgt
      361 atcgcagcat ttcaggccca tcgatgagca tgagaagggg agttttctta aacgaggcca
      421 gataggcgtc aataatggct ttttgatttt ccggggtggg catttttgct gggtccgagc
      481 gactcatgtg ccattctccc caccatccca cagagcccaa atccacgtga tccaagtcgg
      541 ggtgaccgtc ataccgtgcc cccaaccggc ggatgaagtc aagatgggcc cgcaggacca
      601 cagggtcgtc gagatcggga acctccagcc cctcggtgtc gccatatctt gtggtgatga
      661 cacgtccacc gatggccttt agccaatcgg gatggtatgg tcgcttaggc gttgtcgagc
      721 aacacatgac acgaaacgcc agtcgctggc ccgcacgacg tgactcggcc agttgttgat
      781 cgagaaactc gtaatcaatt tcgcccggac gcggttcgag tcgcccccac ccccaacgag
      841 cgtactgaac ggttgaggga atccattccg gcaagttttg atctcgatcg gcggtacgat
      901 ggaacgtctc ccaacccata tgtggattgg gaaggatagc atcgatctcc ttcggtttga
      961 ccgtcgtcca ttcttcggca gaaacagtcc gcactccatg aaatgccagt gctaggcaaa
     1021 caccgaaaac aatagtgacg tgcagtcgca ttgaagaccc tttcaccaat tttgattgcc
     1081 taccatcttt ctgaaaaacc cagaccattc accctagcca aacaagctcc gacgttcctt
     1141 tgggcaaatc gaaacaaagt gtcattaacc actcggtacc gttcgttccg cttccttcgc
     1201 aatatgtgcc agacgtcaag tcaagcagac ggccaactcg tcgcaaccgt tgctgttcct
     1261 cgcccgcccg accaactgcc gggacaccct ccgccagagg tgtcaactgg atcgtaaaca
     1321 aaggtgccga aaacggggca gaaaagacga ccttcgtatc gttcttctcg attcttgtca
     1381 gctcccgggc cgcccaatag cgggcgattt ccgctagatt catccagatc aggttttcgt
     1441 accgagcatg tagtcgttgg acgaccgtct tcagaatctc aaaaccacgt tcctctccgt
     1501 taaagtaaat ccctggccag tggcaaacca tgatggccgg ttcttcacga ccgatcacat
     1561 ccaccatccg tcccttcgct aaatctggtg tgatgaaaag atcggcggac ccctccgtgg
     1621 tcccatccca gccaccaaac cagtcacccg tgcagcctat aatcgacaca acacacttag
     1681 gatcatctcc atcaagcccg gaggcatatt cgacccgggg tgccacactt ctctcgtcgg
     1741 tgaaaaggtc acgaaagtaa tgcggaatct ccgtgcgaaa cacgtcccga cacgcctcca
     1801 gtgtggcctg ggcaagttgc ggacgggcac ggctaccaaa cccaccgggt gtcgttatcc
     1861 cttcgcaatg cagatcgaca ttctttaaga ttcgcaacgc atatgccagg tagtccgtta
     1921 gctcatctac ggaacgcccc tcgctccatc cccaattctc cataaatcgg gctgaccgct
     1981 cctcatatgg ccgtcctgtc ttcgtattga taacccaggt atgggtaacc atctcgggat
     2041 gaatatccca ccggggcata atccgttccc ggactagccg aaggctttca acaagctcct
     2101 ggtgcgacca gcccggcagg tcacgatcta gccaaccgac acaagccgga taaggaacaa
     2161 cgctgtactt tcctttgacc ccgtgctcct cgcaccagtc cataa
//
LOCUS       DAOPTR010000703         2205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39010_length_2205_cov_3.010698, whole genome shotgun sequence.
ACCESSION   DAOPTR010000703 DAOPTR010000000
VERSION     DAOPTR010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39010_length_2205_cov_3.010698"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..1932)
                     /locus_tag="PKI05_12340"
     CDS             complement(226..1932)
                     /locus_tag="PKI05_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008701746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOA53032.1"
                     /translation="MTETLKVSAEYRIPAIRELCYQVVRFTPSAKKIEQTNRAEALLS
                     EIKPDKWYSYGWICYKITRFRPDNAGEQFLGEDLRHDLILFIEDVAETVPLRPEDVSE
                     KIYTLQELAKDFNVSTKTITRWRRAGLVSRRFLIDGRVRVGFLESTVKRFASQEHTRI
                     NRASQFSQVSPEERQAIIERARRLAQAGACRSEVVKRLAARTGRSMETIRYVLQQYDQ
                     ANPDMAIFPDARGPMSEETKERVYRDYRAGESVDVIAKRYCLTRARVMRVIDEMRAKR
                     IMDLPLDYIPNETFEKVTPEEEKQILGPPPPSPHPTRAAKLPPGLPPYLASLYEVPLL
                     TQEQEVHLFRKMNYLKFKASKLREQLRAEIDARKRPNRALMDEIERLYEESVKTKNEI
                     ISANLRLVVSIAKRHVGPAENFFELVSDGNMSLIRAVEKFDYSRGNKFSTYASWAIMK
                     NFARTIPDEHRYRDRFRTSQNELFTLTQDDRSDQIEQETSQLQREIQLQNILHRLDER
                     ERQIIIRRFGLDREQEPLTLKEVGAELGVTKERVRQLEARAIDKLRKLAEEEKIELSD
                     LE"
BASE COUNT          452 a          623 c          567 g          563 t
ORIGIN      
        1 gttttttcga tcgaccccga gaatggctta ctctcgtccc aagggtttcc cagacggaga
       61 gcgacctttg ggattggctc taagcccctc cattttgtgg cctcatggcc gccgaatcgg
      121 ttggaaccgc tcgttgttgg agtcaggccg atttaatctg gtgcgaccct cggcacagcg
      181 gtggccccac ctcatcaccg cgaaaactcg aacgtattgg ccagtttact ccagatcgga
      241 gagttcgatc ttttcctctt cggccagttt tcgcagctta tcgatcgcgc gggcctcaag
      301 ctgccggacg cgctccttgg tcacccccag ctcggcaccg acctccttga gggtgagcgg
      361 ttcctgttcc cgatccagac caaagcggcg aatgatgatc tgccgctccc gttcatcgag
      421 ccggtgcaag atgttctgga gctggatctc tcgctggagc tgagatgtct cctgttctat
      481 ttgatcagac ctgtcatcct gcgtcaaagt gaacagctcg ttctgactag ttcggaagcg
      541 atcccggtaa cggtgttcat ccggtattgt tcgggcaaaa ttcttcatga ttgcccaact
      601 tgcgtacgtg ctaaacttat tgccgcgaga ataatcaaac ttttctacgg cccgaatcag
      661 ggacatgttg ccatcgctca ccaactcaaa gaaattctcg gcgggaccaa cgtgccgctt
      721 ggcgatggac acaaccaatc gcagattagc ggagataatc tcgttctttg tcttgacaga
      781 ttcttcgtaa agccgctcga tctcgtccat gagggcgcga ttggggcgct tccgggcgtc
      841 gatttcggct cggagttgtt cccgaagctt gcttgccttg aacttgaggt aattcatttt
      901 gcggaagagg tgtacctctt gctcttgcgt caagagaggg acttcataaa gactcgcgag
      961 atagggcggt aatcctggag gtagtttggc cgccctagtc ggatgaggcg aaggtggagg
     1021 cggtcccaaa atctgttttt cttcctccgg tgttaccttt tcgaatgtct cgttggggat
     1081 gtaatctagc ggtaaatcca tgatgcgctt ggcccgcatc tcgtcgatga cgcgcatcac
     1141 ccgcgcccgg gtcaaacagt agcgtttggc gatgacatcg acggactccc ctgcgcgata
     1201 gtcccgatag acgcgctcct tggtctcttc gctcatcggg ccgcgcgcat ctggaaagat
     1261 ggccatgtcc ggattggcct ggtcatattg ttgcaacacg taacggattg tctccatact
     1321 tctccccgtc cttgcggcta gacgtttgac gacttcggag cgacaggccc cggcttgggc
     1381 cagtcgcctt gcccgttcaa taatggcctg cctttcctcc ggcgagacct gactaaactg
     1441 tgatgcacga ttgattcttg tgtgctcctg cgacgcgaat cgcttaacgg tgctttccag
     1501 aaaacccacc cgtacacgac cgtcaatcag gaaccgacga ctcaccaagc cggcacggcg
     1561 ccaccttgtg atagtctttg ttgagacgtt gaaatctttg gcaagttcct gaagcgtgta
     1621 aatcttttct gaaacgtctt ccgggcgaag aggaacggtc tctgcaacgt cctcaatgaa
     1681 caggatcaaa tcatgccgaa ggtcttcgcc caggaattgc tcccctgcgt tgtctggtcg
     1741 aaaccgcgta atcttgtagc aaatccaccc gtaagagtac cacttgtccg gcttgatttc
     1801 gctcaggagt gcctccgccc gattcgtttg ctcaatcttc ttggcagagg gtgtgaaccg
     1861 aacaacttgg tagcacagct ctctgatggc cgggattcgg tactcagcgc tgactttcag
     1921 tgtctcagtc atcggtgtca tccgatcgcc ttacccgcaa agtgttttcc ccttcctggt
     1981 attcgcccca acctccccgt aataggacgt caattaccat gaaatggttc gaggtcttta
     2041 tgcaattatg tcagattgat ttgatacaat agagtttaca cggcccacgc atatcgtcaa
     2101 agaccttctg tgccaaaaac gatcgcactt ttgtgcccag gatgggccag tccagagcgt
     2161 cccccccaac cgcactatgg caatatacag aaagccgaac agggt
//
LOCUS       DAOPTR010000704         2203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39059_length_2203_cov_2.701117, whole genome shotgun sequence.
ACCESSION   DAOPTR010000704 DAOPTR010000000
VERSION     DAOPTR010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39059_length_2203_cov_2.701117"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="PKI05_12345"
     CDS             <1..564
                     /locus_tag="PKI05_12345"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HOA53033.1"
                     /translation="GVRIRFDARSHFSYDEALRFCPGIQALGLDMVIDFLKTKSLDSY
                     QRLAWEMDCPLAIRLHDPEELWGTVKSQSVHHLILDMTEFGGVLSVRDAIAVATAAGI
                     GVSLTVSTSLGLTVASMLHVGAATPSLVSAHEIVCEETLHLAIQSGLKMKDGLWEVPN
                     APGLGIEVSREILEQETWSHDLWDFPE"
     gene            691..1158
                     /locus_tag="PKI05_12350"
     CDS             691..1158
                     /locus_tag="PKI05_12350"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOA53034.1"
                     /translation="MAIFFYKLRCPRCGHVEVLSRYDAQRRLQAAKKLRPGVETDEAL
                     LDELVPALLRQIKCEACHEACVEVTVQQERDEWSDEKKCAICGKIIPVLRLALLPDAE
                     LCAECQSAIDRGESPGPAEYCKRCGRPMKLAPRRIGGLTRYVWVCTNDPPCRS"
     gene            complement(1218..>2203)
                     /locus_tag="PKI05_12355"
     CDS             complement(1218..>2203)
                     /locus_tag="PKI05_12355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOA53035.1"
                     /translation="ALGDVVLTTPVACELRRHFPEAHLAWAVESSFAPVLEHHPALDE
                     MIVLPRGWAKSWRSLLKVWREIRARRFDLVIDVQSLSRSGLVGWFTRAKTRIAFAPPQ
                     GREIAPWLATHRCAPTQTHVVDRFLELLTLVGINRPEVTFGLKETLDEASWAEGTLER
                     MGLGQPYAVINPGAGWPSKLWSPDRFAQVAEYLNRVHHLPVLVVWGNQAEREWAEIIA
                     DLGGNRTYLAPPTTIRQMMSLLRRASLMIASDTGPLHIAAAWGTPCVGLYGPTNGEET
                     GPYGTRHIVVQAARYRGKHRRRAPRAIMEAITVDMVCQACAQLLSEAKAAA"
BASE COUNT          488 a          622 c          620 g          473 t
ORIGIN      
        1 ggcgtccgta ttcgtttcga cgcgcgcagc cacttctcgt atgacgaagc cctgcgcttt
       61 tgccccggga ttcaagccct agggttggat atggtgatcg attttctcaa aacaaaaagc
      121 ctggattcct atcaacggct cgcatgggag atggattgcc cgctggccat tcgactgcat
      181 gacccggaag agctttgggg aactgtcaaa tcacaaagcg tccaccattt gatcctggat
      241 atgaccgagt tcgggggggt tctatcggtt cgggatgcca tagccgtggc gacagcagca
      301 gggataggtg tttcgctaac cgtaagcaca agtcttggtc tgaccgtagc aagcatgctc
      361 cacgtcggtg cggcaacacc gagtttagtg tccgcgcatg agatcgtttg cgaggagaca
      421 ttacacctgg cgatccaatc cggcttaaag atgaaagatg gcctttggga ggtacccaac
      481 gcccccggcc tagggattga agtaagtcgt gagatattgg aacaagaaac ctggtcccac
      541 gatttgtggg atttccccga gtaacttttg agcgtcgcgc cgttggtggc aatccacgcc
      601 cgatgtctca aagtagagtc actcgaatgt cgatccgaag gtcttttggc caagcaaggg
      661 tacaacaaag tcctgaggaa gcgtttcaat atggcaatct ttttttacaa gcttcgctgt
      721 ccccgttgcg gtcacgtgga ggtcttgtcg agatacgacg cccagcgacg actccaggcc
      781 gcgaagaaac tccgccctgg tgtggagacg gatgaagccc tgttggacga gttggtcccg
      841 gccctgttac ggcaaatcaa gtgtgaggcg tgccacgagg catgcgttga ggtaaccgtt
      901 caacaggagc gggacgagtg gagcgatgag aaaaaatgtg ccatttgtgg aaagattatt
      961 cccgtattga ggcttgctct tctgccggac gccgaattgt gcgccgagtg tcaaagtgcc
     1021 atagaccggg gagaatctcc aggaccagcg gaatactgca aacgttgcgg ccgaccgatg
     1081 aagctcgctc cacgacggat tggtggttta acccgttatg tttgggtttg tacgaacgac
     1141 ccaccttgcc gctcttgaac gcttatctgc tgaagcttgt ctgaattggc acttccacat
     1201 ccaccgttaa actcttttca ggccgccgct tttgcctcgc tgagcaattg cgcgcatgct
     1261 tggcagacca tatccacggt gatggcctcc atgatcgccc gaggggctcg ccgtcggtgc
     1321 ttgccgcgat aacgggcagc ctgcacaacg atgtgcctcg ttccgtaagg ccccgtttct
     1381 tcgccgttag taggcccgta caaaccgaca cagggcgtac cccatgcggc ggcgatgtga
     1441 agaggccccg tgtccgacgc aatcatgagc gatgcccgtc gcaaaagtga catcatctgc
     1501 cgaatcgtgg taggcggcgc aagatatgtc ctattccccc ccaaatccgc gatgatctct
     1561 gcccattccc gctcggcttg atttccccac acgacgagca ccggcaaatg atgaacacga
     1621 ttaaggtact ccgcaacctg ggcaaaacgg tcgggagacc atagcttaga cggccaaccc
     1681 gcgcccgggt tgatcacggc atacggttgg cccaaaccca tccgctcaag ggttccctcg
     1741 gcccagctcg cctcatcaag ggtctctttt aagccgaacg taacttcggg gcggttgatg
     1801 ccaaccaatg taagcaattc taaaaatcgg tccactacgt gcgtttgggt gggagcacac
     1861 cggtgagttg ccagccacgg ggctatttcc cttccctggg gtggtgcaaa agcgatacgg
     1921 gttttagctc tcgtgaacca tcccaccaag ccactccggc tcaggctttg cacgtcgatg
     1981 accagatcaa accggcgggc gcggatctct cgccaaacct ttagaagact ccgccacgat
     2041 tttgcccagc cccggggaag cacaatcatt tcatcgaggg ccggatggtg ctccaacaca
     2101 ggggcgaagg acgactccac ggcccaggca agatgagcct ctggaaaatg ccgacgcaat
     2161 tcgcaggcca cgggtgttgt caggacaaca tcgcccagtg cgc
//
LOCUS       DAOPTR010000705         2200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39117_length_2200_cov_4.618182, whole genome shotgun sequence.
ACCESSION   DAOPTR010000705 DAOPTR010000000
VERSION     DAOPTR010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39117_length_2200_cov_4.618182"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="PKI05_12360"
     CDS             complement(<1..979)
                     /locus_tag="PKI05_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA53036.1"
                     /translation="MQVSKTQLSRRRFLRTVMLGSGVAVPMLVSPRAMGFAGLAPASE
                     RIILGAIGIGNRGTYVLACFLQENDVECVAVADVKLFRREQAKRMVDERNGNTNCKTY
                     RDFRELLDRPDIDAVLIATGPNWHWLASVLAAKAGKDVYCEKPCTKNIMQSLSLAETF
                     RRTGRVFQAGTQRRNLPHFAFAVELAQKGRLGKLHTLYAHPAGMSTSMSGWAPPEPEP
                     NKEEIDWDLYLGPAAWRPFNKRFLDGFNFEKGGGLVGGGVLEWGSHCVDLCQWANQAD
                     HTAPVEYFPPENGQVSAVYANGVRLILRETGWLPLGSCPVRFEGDKGWVE"
     gene            complement(1398..1961)
                     /locus_tag="PKI05_12365"
     CDS             complement(1398..1961)
                     /locus_tag="PKI05_12365"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOA53037.1"
                     /translation="MRSPLQKSLSEHVGWVVLLTAIVGSTVAFANNSAQPTPTPPGHL
                     RPRAVDISLSQNGVFSGKVVDAQARPVEKAEILFRQSGKQVAKVSTSRNGDFDVTGLT
                     PGVYQIVVLPDRALTVRVWNKRSAPPVARPELLIVIGDDTIRGQRRIGELLPLENTVV
                     VGGMVAAAIAIPIAIANSSDDTEPVSP"
BASE COUNT          520 a          642 c          588 g          450 t
ORIGIN      
        1 cttcgaccca acctttgtcg ccttcaaacc gcacggggca agagcctaac ggcaaccagc
       61 cggtttcccg cagaatcaat ctcacaccgt tggcgtaaac ggcagaaacc tggccgtttt
      121 ccggtgggaa atactcaacc ggggccgtat gatccgcttg attggcccat tggcagagat
      181 cgacacaatg ggaaccccac tcgagaacac cgccgccgac aaggccaccg cctttttcaa
      241 agttaaaccc atcgagaaac cgcttgttga aaggccgcca cgctgcgggg ccaagataaa
      301 ggtcccaatc gatttcttct ttgttcggct cgggttccgg cggtgcccaa ccgctcatgc
      361 tcgtactcat tcccgcggga tgggcataaa gggtatgcag cttgcccagc cgaccttttt
      421 gggccaactc caccgcaaat gcgaaatggg gaagattccg tcgctgcgtc cccgcttgaa
      481 agacgcgacc ggtccgacgg aacgtctcgg ccaacgacaa actctgcatg atgttcttgg
      541 tgcaaggctt ctcgcaatac acgtccttgc ctgccttcgc cgccaaaacc gaggccaacc
      601 agtgccagtt cgggccggtg gcaattagaa ccgcgtcgat gtccggccga tcgagcaatt
      661 cacggaagtc tcgataagtc ttgcaatttg tattgccgtt tcgctcatcg accattcgtt
      721 tggcctgttc ccgccggaac agtttgacgt cagctacggc cacacattca acgtcgtttt
      781 cctggagaaa acaggcgaga acgtacgttc cccggttacc aatgccaatg gcccccaaga
      841 taatccgctc gctggccgga gcaagtccgg caaagcccat cgcacgcggc gataccaaca
      901 tgggcacggc gactccactc cccagcatca ccgtgcgaag gaaccgccgg cgggaaagct
      961 gtgttttgga aacctgcatg ccaactctcc ttttttagtc ttgcataaag atgcactcaa
     1021 agaccaaaca tgggccatgg ttaccaacac aggcccacat gccatctcat cccggaacac
     1081 cagcgtgcaa cccaaaaaag ggtgggaaca cttgcacgag ggaggtaaag accaacccag
     1141 tgaatggtta cagtccgcga ttgatgatgg tctgaaacga caccgttgtc aagtgttacg
     1201 ccgggaacgc aaaagtgctt cgccggcgca acattgaaat tcgttctagc cgctgatttc
     1261 ctttttggac atgcgatcag gactgtcaga ggacctccga taaggggaat ggggcgattt
     1321 cgaaatcaaa ctgagggcgc atgatgagcc ctcgacttcg acgtgttatc agtctgggga
     1381 acgcggaagg aggatactca cgggctgaca ggttcggtat cgtcgctaga gttagcgatc
     1441 gcaattggga tggcaatggc tgctgccacc atgccgccga ccaccaccgt attctctagg
     1501 ggcaagagtt caccgattcg gcgttgtccc cgaattgtgt cgtcgcctat cacaataagc
     1561 agctcgggac gtgctaccgg cggggccgac cgtttgttcc aaacacgcac cgtgagggcg
     1621 cggtccggca gtacgacgat ctgatagact cctggcgtta acccggttac gtcgaagtct
     1681 ccgttgcgac tggtagaaac cttagccacc tgcttgccgg actgccgaaa cagaatttcg
     1741 gccttctcaa ccggtcgagc ttgggcgtca acgacttttc cggaaaaaac gccgttttga
     1801 ctcagcgaaa tgtccacagc gcgaggtcgc aaatgtccag gcggagttgg tgtcggctgt
     1861 gcagagttgt tggcaaaggc caccgttgag ccaacgattg ctgttagcag cacaacccac
     1921 ccgacgtgtt cgctcaagga tttctgcagt ggggacctca tcacaaacct ccatactccc
     1981 ttcggaacgt tagctacaag gccaatggct taggctaggg aatcgccttc acggttcccc
     2041 tgattcgttc gtaactgtgt cgaaaaccgg gcgaacctta gcaacccagg tcggttccat
     2101 caagagcaat tcgcacacca aaaaccgttg tcgggataag gtgggaggga ataggggact
     2161 acgcaagacc cgccattaga aatgggcaag atccggaccc
//
LOCUS       DAOPTR010000706         2200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39121_length_2200_cov_3.972028, whole genome shotgun sequence.
ACCESSION   DAOPTR010000706 DAOPTR010000000
VERSION     DAOPTR010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39121_length_2200_cov_3.972028"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..>1414)
                     /locus_tag="PKI05_12370"
     CDS             complement(62..>1414)
                     /locus_tag="PKI05_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOA53038.1"
                     /translation="ARTVINLAPADLPKEAASFDLPIGLGILAASGQLTPERLAEYAV
                     VGELALDGTLRPVRGVLSMAMQLKKEGGCRGMIVPVANAPEAAIVEEIDVIPVSSLSQ
                     AAAFLCGEIDIPPQPSRLEEFFDKAAHYEEDLADVKGQEMAKRALVVAAAGGHNLLMI
                     GPPGSGKTMLARRLLTILPELTPAESIETTRIYSAVGKVNPEQPLLATRPFRAPHHTI
                     SDAGLVGGGSIPGPGEISLAHNGVLFLDELPEFNRRTLEVLRQPLEERSVTIARANFS
                     STFPANFMLVAALNPCPCGYRNDPRRQCHCTIPQIERYMSKISGPLLDRIDIHLEVPA
                     VPFHDLMASRPGLGSAEARAMVTQARKIQMQRLNGTKARCNAEMTHQQLRRFCRLSPG
                     SRALLQAAVNELGLSARAHDKILRVARTIADLEGAEEINEMHLSEAIQYRTLDRSFFK
                     "
     assembly_gap    1415..1424
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1425..1606)
                     /locus_tag="PKI05_12375"
     CDS             complement(<1425..1606)
                     /locus_tag="PKI05_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HOA53039.1"
                     /translation="MLAKLKTFTLLGIDALPVVVEVDVSPSGLPKTVIVGLPEASVRE
                     STHRVERAMVNSGFHRA"
     gene            complement(1818..>2200)
                     /locus_tag="PKI05_12380"
     CDS             complement(1818..>2200)
                     /locus_tag="PKI05_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53040.1"
                     /translation="GTKGTCYLGQKRIVGETNWRYEGPQNNPYQDEQQALFDSIRKGQ
                     PINSGYHMAKSTMVTVLGQLAVYTGQAMRFEDVAKMDFQYRPTVEEARLDMEPPTVPD
                     ETGNYPLPKPGISSLEWIAGPRRT"
BASE COUNT          447 a          643 c          620 g          480 t
ORIGIN      
        1 gcaattttcg gtattataag ggatttccgg ctttccgacg accggtccca aacttctcac
       61 attacttgaa aaagctgcgg tcgagggttc gatactggat cgcctcgctg aggtgcattt
      121 cgttgatttc ctcagcccct tccaagtcgg caatggtcct cgccacacgc aagatcttat
      181 catgggctcg tgccgaaagc cccaattcgt tcaccgccgc ctgtagaagc gccctcgaac
      241 cggggctaag gcgacaaaac cgacggagct gctgatgcgt catctcggcg ttacagcgtg
      301 ccttggtccc gttaagtcgc tgcatttgaa tctttcgtgc ctgagtcacc atggcccggg
      361 cctccgcgct gccaagtccg gggcgcgagg ccatcaagtc atgaaacggc accgccggga
      421 cctccagatg gatgtcgatc cggtcgagca aaggcccact gatttttgac atgtaacgct
      481 caatctgtgg aatggtgcaa tggcattgcc gccgaggatc attgcgatag ccgcagggac
      541 agggattgag ggccgccacc aacataaaat tggcgggaaa ggtggagctg aaattggccc
      601 gtgcgatggt cacgctccgc tcctcaagcg gttgcctaag cacctccaat gtccgacgat
      661 tgaattccgg gagttcgtcc aaaaaaagga cgccattatg agcgaggcta atttcgccgg
      721 gaccggggat cgatcctccg cccaccagtc ccgcgtcgct gatggtgtgg tgcggggccc
      781 gaaagggccg ggtcgccaat aggggctgct caggattgac cttccctacc gcgctgtaga
      841 tacgtgtggt ttcaatcgat tcggcgggcg ttagttcagg aaggatcgtc aacagccgcc
      901 tggccagcat cgtttttccg gagccgggcg gcccgatcat gaggaggtta tgccctcccg
      961 cagccgccac gaccaaggcc cttttggcca tctcctggcc tttaacgtct gccaggtctt
     1021 cttcgtagtg cgcggcttta tcaaaaaact cttcaagacg cgagggttgg ggcggaatgt
     1081 caatctcacc acagagaaat gcggcggctt gactgagtga ggaaacgggg atgacatcga
     1141 tctcctccac aatggcagcc tcgggggcat tggcgacggg cacaatcatg cctcggcagc
     1201 caccctcttt tttcaattgc atcgccatgg aaaggacacc gcgcacggga cggagcgtcc
     1261 cgtcgagcgc caactctcca acgaccgcgt actctgccaa acgttctggg gtaagttgac
     1321 cgctggccgc gagaatcccc aagccaatgg gaaggtcaaa cgaggctgcc tctttgggaa
     1381 gatccgcggg tgccaggttg atcacggtcc gggcnnnnnn nnnngcccgg tgaaagcccg
     1441 aattgaccat cgcccgctcc acacgatgtg tgctctctcg caccgaggcc tcgggcaagc
     1501 ccacaatcac agtcttgggc aatccggatg gcgagacatc gacctccacg accaccggca
     1561 gggcatcgat tcccaatagg gtaaatgtct taagcttggc gagcataacc tggttgcccc
     1621 ctggtttggc tttccattaa atgacaatgt ctcctatttt cagaaggccg ggaaacctag
     1681 gcaaggtgtc ggatgctaac aaggaaaggc ccccaaacct tggatttgga ggcctttttc
     1741 ctttgggcat cgtcctgtct gttattagcg gggcacggcc actgggatta acgcggtccg
     1801 tgctccgcct tcaatgatca agtccggcgt ggcccggcaa tccactccaa actgctgatc
     1861 cctggctttg gcaacggata attccccgtc tcatcgggaa ccgtcggcgg ttccatatcg
     1921 aggcgggctt cctcgaccgt cggccgatat tggaagtcca tcttggccac gtcttcaaac
     1981 cgcatcgcct gaccagtata gacggcgagt tgtcccagga ctgtcaccat ggtgcttttg
     2041 gccatgtgat atccgctgtt gatgggctgg cccttccgga tcgagtcgaa aagggcctgc
     2101 tgctcatcct ggtaaggatt attttgtgga ccttcgtaac gccaattcgt ctcgccgacg
     2161 atccgttttt ggccgagata acaggtccct ttggtcccca
//
LOCUS       DAOPTR010000707         2198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39168_length_2198_cov_2.398973, whole genome shotgun sequence.
ACCESSION   DAOPTR010000707 DAOPTR010000000
VERSION     DAOPTR010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39168_length_2198_cov_2.398973"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="PKI05_12385"
     CDS             complement(<1..1012)
                     /locus_tag="PKI05_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53041.1"
                     /translation="MSHRVPHLGLRYLFVFSGLLTPVLFAYLIFSPGALLGAAQQASP
                     DYVAPLVLAEGKADATKAEQPSTSPSGRSQSRGKKQRPQTAEGADESAGKTSSGEQKA
                     NRPQFVRLLVRKNEPQYLQTAIVSYVVRDDSSQGKTLSAGETGRSQPAWPKDLRVDLV
                     GVIHIADRGYYEQLNKILRKYDVVLYELVAPQGTRVTPGQRPAHPVSALQQSLRNMLG
                     LAYQLDVIDYHRPNFVHADLSPEEFYQSMKEKGESTWAMLLRAFGYEWARSLSRQSTP
                     NDADLVRALFSKDRQLQLKRLFAQQLSEDVEGQIRALEGPQGYTLISGRNERVLAVLE
                     EQA"
     gene            2009..>2198
                     /locus_tag="PKI05_12390"
     CDS             2009..>2198
                     /locus_tag="PKI05_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53042.1"
                     /translation="MLANLWASLRQARNKSHSSRERRRLRVDALEERTLLSVTPVDIL
                     DKLVNQSLIPSGGTIAGQS"
BASE COUNT          465 a          577 c          616 g          540 t
ORIGIN      
        1 tcgcttgctc ctccaacacg gccaagaccc gctcgtttcg cccgctgatg agagtgtatc
       61 cctggggacc ttccaatgcc cgaatttgac cttctacatc ctcggataat tgctgcgcga
      121 aaagtcgctt cagttgaagt tggcggtctt tgctaaacaa agcacgtacc aagtcggcat
      181 cattcggcgt gctttgtctt gaaagactcc ttgcccattc gtaaccgaag gcccgaagca
      241 gcatcgccca ggtgctttcg cctttttcct tcatggattg ataaaactct tccggcgaga
      301 gatcggcatg aacaaaattg gggcgatggt aatcgatcac gtcaagctga taggccaggc
      361 ccaacatatt tctcagagat tgttggagtg ccgagaccgg gtgggccggc cgttgtcctg
      421 gtgtaacccg cgtgccttgc ggggcaacca attcgtagag gaccacatcg tacttgcgaa
      481 gaatcttgtt gagttgttca tagtagcctc tgtctgcgat gtggatgacc ccaaccagat
      541 ccaccctcag gtctttaggc caggcaggtt gcgagcgacc tgtttctccc gcggaaagtg
      601 ttttgccttg tgatgaatca tctcgaacca cataactgac gatggctgtt tgaagatact
      661 gaggttcgtt tttccgcacg agcagccgga cgaattgagg ccgattggcc ttctgttccc
      721 cggaggacgt tttccctgct gactcgtcgg ccccctccgc ggtttggggt cgctgcttct
      781 tcccgcgact ctgtgacctc ccggaaggag acgtggaggg ttgctcggcc tttgttgcgt
      841 ctgctttgcc ttcagccagt accagcggcg cgacgtagtc tggactcgcc tgttgggctg
      901 cacctagtaa cgcgcccggg gagaaaatga ggtaggcgaa gagcacgggc gtgagcaacc
      961 cgctaaagac aaacagataa cgaagcccaa gatggggaac ccgatggctc atattcgttc
     1021 cctctaccat gcacgagttt tgccgagaac acaacccttt gccttagata gcttcttgac
     1081 cggcccgagg tcgcttcctc catgtggctc aaagcaactc atcgccagaa aaagcttcaa
     1141 ggcgccctgc ttattgtacc atccccagga cggtgaaata acgttttttg actctacccc
     1201 cttgccggcg gggttggaag tacaacggat tataacggcc ggatggtgtg tctttatcgc
     1261 atcaggatcg actcttggat actccgaagg cgggcgattt ctgtgtcaag cggctgcttg
     1321 tattttttgt gaccgcgcgg ttgaggcgtg tcgctgctcc gacgggatgt ctgccttcga
     1381 ttttccttca ggataatttg agacgaacga tccggtatcg gcaaactcac cgccatactt
     1441 gacaaaaatg gaacttcccg ccgcttcgcg cgatcaaagt ggtacaggga ccagcacttt
     1501 gggtcagggc gccctttagt aatccggttt tcaaacgctc atgagatcca caaacggggt
     1561 ggttaaaggg agcgcttggc cggatgtctc ttattttgga cccggtcatg gcaagagaag
     1621 cttcggttaa tgtcccatcc cgcgtatgga ccacgccgat cgtggcgttg acttgacgct
     1681 ttaggatgcc gtaatcatga taaaacagtc gagatggcta ttttagcaaa gatatgcaag
     1741 gtggccgcca gttcctattg ccgggtgatg atcgcttcaa aggaggccgt gcttttctac
     1801 tcaaagagga gttgggtgat ccggtgggaa cgtcatatgg gtggtcttac ggagtgactg
     1861 ggcacaccac agtagggggt aatggggtgg cgatcaagag gcgagtgtga acaccaagtc
     1921 agcacttttt gacctttggc agactaacgg ttgaggacat ctcactatgt tgaaacagcg
     1981 ggttgctcgt cagcgaagga agggttctat gcttgccaac ctgtgggctt cgttgcgtca
     2041 ggctcgaaac aaatctcatt cctcacggga gcggcgacgc ctgcgtgttg acgccttgga
     2101 agagcgtacg cttctcagcg tgacgcccgt ggatattctc gacaagcttg tgaaccagtc
     2161 cctgatccct tcaggtggga cgattgccgg gcaatccc
//
LOCUS       DAOPTR010000708         2197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39181_length_2197_cov_3.394958, whole genome shotgun sequence.
ACCESSION   DAOPTR010000708 DAOPTR010000000
VERSION     DAOPTR010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39181_length_2197_cov_3.394958"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1424
                     /locus_tag="PKI05_12395"
     CDS             81..1424
                     /locus_tag="PKI05_12395"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOA53043.1"
                     /translation="MALVLSALPFLIVMGLLGVMSACFSAAEAAFFSLRKQDQAALRR
                     SGAAGQLVIGLLKDVEQLLTAILFCNPLVNIAYFAVSSIVTLTWQRMGATAEAGVFAS
                     VSVLSLILFSEMFPKSVAISWPQGVAQLLAVPIWIAVRLTQPWLPFFRSAAITIRRIL
                     WPRFRPEPYLRVRDLEQAFDFSRESGVVLEQEHQILQNLVQLSELKSEEVMRPRTEVP
                     IFHRPLQRDQLGQELPSSGYFLIVEDEDEAFTRALSPDQLLQALADQPEDGPSATKTD
                     SESCPGADQTAEDLAKWAEPVVYVPWCVSAAQVLDLLVEENRAVAAVVNEYGETVGII
                     TLDDLLRRIFAPESSRSESLYKRRSIQNVGPNVWHVTGLTSVRRLARYFGIDPPPGSS
                     LTVGGIFQEKLGRVAKQGDTCTWGPFVLKVLQMGRHGAVLVEVRPSQEGPKEVGE"
     gene            1421..>2197
                     /locus_tag="PKI05_12400"
     CDS             1421..>2197
                     /locus_tag="PKI05_12400"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOA53044.1"
                     /translation="MIVLGLICFAVGLFLSALFSGSETGFYRVARLHLRVAAVTGDWI
                     SQAILWVSYHPSLFIATALVGNNLANYLLSFATVLLAQAFTASPQGWTEILATMIMAP
                     VAFVLGELLPKRLFLLAPFTLLRYTGPLFLVFTVVLAPVSVILWIWNRFLALLIPTPP
                     EIAQSQIRGWELRHIVEEGEHAGLMTNSQVRLANVILQAGTRPIKEWMHPLGELATVP
                     LDAPPEKNAELLCRGKRFLLVTHPQAAHRVVGYVDLAQWVL"
BASE COUNT          385 a          628 c          654 g          530 t
ORIGIN      
        1 gaacgtcgtt ggacgaaaca tgaccatgga agtgatggcg gaaacgacat cgtcccttgg
       61 ttgaggagtc atacgagatc gtggcgctgg tgctatcggc cttgccgttt ctgattgtga
      121 tggggctgct tggagtgatg tcggcctgct tctccgcggc ggaggctgcc ttcttttcgc
      181 tccgcaaaca ggatcaggcc gcactccgac gaagtggcgc cgccggtcag cttgtgattg
      241 ggctactaaa ggatgtggaa cagcttttga cggccatcct cttttgtaat cccctcgtca
      301 atattgccta ttttgccgtt tcctccattg tcacgttgac ctggcagcgg atgggagcca
      361 cggccgaggc cggtgtcttc gcaagtgtct cggtgttgtc gctgatcctt ttcagcgaaa
      421 tgtttcccaa aagcgtcgcc atctcttggc cgcagggcgt cgcccagctc ctggccgtcc
      481 ccatctggat cgccgttcgc ctgacgcagc cttggcttcc cttctttcga tcggctgcca
      541 tcacgattcg ccgcatcctt tggccgcgtt ttcgaccgga gccttatctt cgcgttcgcg
      601 acctggagca agcctttgac ttttcacgcg aaagtggtgt ggtcctcgag caggaacatc
      661 aaatcctgca aaatcttgtg cagctctccg agctgaaatc ggaagaggtc atgcgaccgc
      721 gaacggaggt gcccatcttt caccgtcctt tgcagcgaga tcaacttggc caggagctac
      781 cttccagtgg ctacttcttg atcgttgaag acgaggacga ggcctttacc cgggcgctct
      841 cccctgacca attgcttcag gcattggcgg atcagcctga ggatggtccc tcggcgacaa
      901 aaacggactc tgaatcgtgt cccggtgccg accagacggc agaggacctc gcgaaatggg
      961 cggaacccgt cgtgtacgtc ccgtggtgcg tttccgccgc ccaagtcttg gacctcttgg
     1021 tcgaggagaa tcgggcggtt gcggcggtcg tcaatgaata tggggaaacg gtggggatta
     1081 tcacgctgga tgaccttttg cggcggattt ttgccccgga aagcagtcgc agcgagtcgc
     1141 tttacaagcg acgttccatc caaaatgtgg ggccaaacgt ctggcatgtg acgggcctga
     1201 cgagtgtgcg gcggttggcc cgctactttg gcatcgatcc cccgcccggc tcgagcctga
     1261 cggttggtgg gatctttcag gaaaaactgg gcagggtggc caagcagggt gatacgtgca
     1321 cctggggacc cttcgtcctt aaggttctac aaatgggtcg ccacggggct gtgctggtag
     1381 aagtcaggcc gtcccaggag ggaccgaagg aggtcggcga atgatcgtcc tgggacttat
     1441 ctgctttgcc gtgggattgt tcctcagcgc cctatttagc ggttccgaga cgggttttta
     1501 ccgtgtagcc cggctccacc ttcgggtggc cgccgtcacg ggtgactgga tttcccaagc
     1561 tattctctgg gtttcgtacc atccctccct ctttattgcc acggccttgg tggggaataa
     1621 cctggccaat tacctcttat cgtttgccac agtgttgctg gcccaggcct tcactgcaag
     1681 tccccaggga tggacggaaa tcctggcaac catgatcatg gccccggtgg cttttgtgtt
     1741 gggggaactg ctcccgaagc ggctctttct tttggcccca ttcacactgc ttcgttacac
     1801 cgggccgctg ttcttggtct ttaccgttgt cctggcaccg gtttcggtga tcttgtggat
     1861 ctggaatcgc ttcctggccc ttttgatccc cacgccgcct gaaatcgccc aatcgcagat
     1921 caggggctgg gaattgcgcc acattgtgga agaaggcgaa cacgccgggc tgatgaccaa
     1981 tagccaggtt cgtcttgcca atgtaatcct gcaagcgggg acgcggccaa tcaaggagtg
     2041 gatgcatccg cttggcgaat tggcaactgt accgctcgac gcaccaccgg aaaagaacgc
     2101 ggagcttctc tgtcggggca aacgctttct gctcgttacc catccccagg cggcccatcg
     2161 cgtggtgggc tacgtggatc tcgcccagtg ggtgttg
//
LOCUS       DAOPTR010000709         2196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39200_length_2196_cov_3.987856, whole genome shotgun sequence.
ACCESSION   DAOPTR010000709 DAOPTR010000000
VERSION     DAOPTR010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39200_length_2196_cov_3.987856"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1737)
                     /locus_tag="PKI05_12405"
     CDS             complement(<1..1737)
                     /locus_tag="PKI05_12405"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOA53045.1"
                     /translation="MVSYQSKRRDDSSRRGWVLKGPLFVLGMMIVGIPVVWSFLPKTL
                     WQGSHEKPLTTTVTRGEFINQVVERGDVESASNVEIRCEVETQGSGMMIIRIVPEGTY
                     VQKGDFLVELDSSTLRDQLVQQQIKVANERALLIQAQRALDTAIIAKQEYLEGQYRQQ
                     RLAIEIDNFIAQENRRRAEDYLKYSQSLAQKGYITQAQLEADQFALEKAIKEMESART
                     KLDVLERFTKPKYLMQLDADIETARAKLEAQQHSLELQEQELKKIQDQIAKCIITAPE
                     SGQVVYANQIGVRGQTDIIIQEGVRVRQYQTIIRLPDPKRMQVKTKVNESKIGLVRVG
                     LPAKIRVDALAGVEFDGEVTDVGEYPLPSSFFNSNVKDYQVIVKINNPNEQLRAGLTA
                     QVTILAQRESNVLQVPVQAVFEYAGEFYCISIDQDGKLTPVKVELGASNDRFVVIRQG
                     LQENQEIVLNAAAYREKVGLAEQKTSPQQAWTEEQAKQASETAAEPAIRTRGPAGEAT
                     EEGAGREQLGQGEGAPRPGGGGPGGFNPAMIADRIFERSDANRDGKLDASEIPEDRRE
                     DLLRHDANGDGVI"
BASE COUNT          435 a          658 c          557 g          546 t
ORIGIN      
        1 gatcaccccg tcaccgtttg cgtcatgccg aaggaggtct tcacgacggt cctcggggat
       61 ttcggaagcg tcgagtttcc catctcggtt ggcgtccgag cgttcaaaga tgcgatcggc
      121 gatcatggcc gggttgaacc cgcccggacc tccgcccccc ggccgcggcg ctccttcacc
      181 ttgacctagc tgctcccggc cggccccctc ctccgtggct tcacctgccg gtccccgtgt
      241 acgaatcgcc ggttcggctg ccgtctcgct ggcctgtttt gcctgctctt cggtccaggc
      301 ctgctgcggc gacgtctttt gctcggccaa tcccaccttt tcgcggtacg ctgcggcgtt
      361 caagacgatc tcttgatttt cttgcaagcc ttgccggatc accacaaatc ggtcgttgct
      421 tgccccgagt tccaccttta ccggtgtgag ctttccatcc tgatcgatgg aaatacaata
      481 gaactctccc gcgtactcga atacggcctg caccggaacc tggagcacgt tgctttctcg
      541 ttgggcaagg atggtcactt gggcggttag tcccgcccgg agttgctcat tggggttgtt
      601 aatcttcaca atcacctggt agtccttcac gttcgaatta aagaacgagc tgggcaaggg
      661 gtattcgccc acgtccgtga cctcgccatc aaactctacc ccagccaagg catccacgcg
      721 aatctttgcc ggcaagccga ctcgaaccag accaattttc gattcattga ccttggtttt
      781 gacctgcatc ctcttgggat cgggaaggcg gatgatcgtt tggtactgcc tgactcgtac
      841 accttcttga atgataatgt ccgtctgacc ccgcacgccg atttggttgg cataaaccac
      901 ctgcccagac tcaggggccg tgatgatgca ttttgcaatt tgatcttgga ttttcttgag
      961 ctcctgttcc tgcaactcta aggagtgctg ctgggcctcc aattttgccc gagccgtctc
     1021 aatgtcagcg tcaagttgca tgaggtactt gggtttagtg aaccgttcca ggacatcgag
     1081 tttagtccgg gcagattcca tctccttgat agccttttcc aaagcgaatt ggtcggcttc
     1141 taattgggcc tgcgtgatgt agcccttttg cgcgaggctc tgactgtatt taagataatc
     1201 ttctgcccga cggcggtttt cttgggcaat gaagttgtcg atttcaatgg caagtctctg
     1261 ctggcggtac tgcccttcca ggtactcctg cttagcgatg atggccgtgt ccagcgctct
     1321 ttgggcctga atcagcagtg ccctctcatt ggcgacctta atttgctgct gaaccaactg
     1381 atcgcgaagc gttgaggagt ccagctccac caaaaaatcg cccttttgga cgtaagttcc
     1441 ttccgggacg atgcggatga tcatcatccc gctgccctgg gtttccacct cgcagcggat
     1501 ttccacgtta ctggcgcttt ccacatcgcc tcgttccacc acctggttga tgaattctcc
     1561 ccgagtgacg gtggtcgtca gaggcttctc gtgggacccc tgccaaaggg tcttgggcaa
     1621 gaaggaccaa acgacgggga ttcccacaat catcatcccg agaacaaaca aggggccttt
     1681 caaaacccaa ccccgccggg aggaatcatc acgacgcttg gactgataac taaccatgga
     1741 aggtctcctt caccgaaatc ccccaacatc ggcgagcccc ttttttcctc aggcgagccc
     1801 tccgcttccg cccaaaaaag cggaaatccc tcgcttagga cggaaattcg ccctgctacg
     1861 gacaatcggg gccgaaaggc aactgcctaa gtttatttcc tcgctggcaa cgactttgcg
     1921 caattgtttc cgatgtggtg ggaatccgtt cgtgcgggct cattcaatgg cgggaaagcc
     1981 gattcgccga ggcggtcacg agccggccca acgacatata gtcatcgccg tcggtccaaa
     2041 ggttggccga cttcagggca gaccctccta aggcaggctt ccccctatct attgtaacca
     2101 gcaaaggccg aaaaagtctt gaaatcggac ccaaaaaggc ataatatcaa ttgtatcagg
     2161 cccaaatgct atatagcctt tttaacaatg gccgtt
//
LOCUS       DAOPTR010000710         2194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39260_length_2194_cov_2.728845, whole genome shotgun sequence.
ACCESSION   DAOPTR010000710 DAOPTR010000000
VERSION     DAOPTR010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39260_length_2194_cov_2.728845"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1511)
                     /locus_tag="PKI05_12410"
     CDS             complement(<1..1511)
                     /locus_tag="PKI05_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53046.1"
                     /translation="MDYWREQTATVQTAFSKSMKQALILFLELLVWFSSIAAVCADGE
                     TTAPEIVNLRVGVEGVYKLGLWTPVWVELRGGTGEASGTIVVTVPDDEGGPCSYVMET
                     VTLPAQATREVEMLVQIGRPRAQATVRWEAEGGPSVEKIFQAGTASFPTAIAARTPVF
                     LWVGPSQPPQALMGRRNEKGHWVWVKNPERLPTHFLGYQSVRTVVLAIGDDKEFIGRL
                     QRSGKQMLALQEWVREGGKVVASFGPTAKTVVSDGGVLSWVAPGTWQNAIPLRKCAGI
                     ETYVDSPRPIVVRRSDPPLQALQFTNFEGIVTASEGNIPLVIRRQYGFGTIVFFTTDL
                     THRALVEWTQFDRLIDRLVGYSVAQDDVAGNPPRTLMHYGYTDVSGQLRSALDVLPTI
                     SFVPFSWVAVLVVIYLVIIGPVDYWILGYGIRNYRWTWITFPAIIILAAGADILLGYS
                     KGERPVTKEVHWIDYDLAGNAIRGLGLGSLYSLRPGFYDISFTAPDFGTKSEVG"
     gene            complement(1589..1936)
                     /locus_tag="PKI05_12415"
     CDS             complement(1589..1936)
                     /locus_tag="PKI05_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008700591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HOA53047.1"
                     /translation="MSETHETVRQETDDRDITFSDFQRLIRKMYFEKDVARGVEGTFM
                     WLVAELGELAEALRSGTAEEKAEEFADVVAWLTTIANVSNVDLTAAIRKKYGQGCPGC
                     GRFVCVCPDAGKP"
     gene            complement(1983..>2194)
                     /locus_tag="PKI05_12420"
     CDS             complement(1983..>2194)
                     /locus_tag="PKI05_12420"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOA53048.1"
                     /translation="LAAQRGVTPREICAVGDDVNDISMIREAGFGVAMGNAPDEVKKV
                     AHWVAPPQEEDGLVAVIERLLEGSL"
BASE COUNT          511 a          696 c          529 g          458 t
ORIGIN      
        1 cccacctccg actttgttcc aaaatcgggc gcagtgaagc tgatgtcata aaaaccgggg
       61 cgaaggctat acaggctacc cagtcctagc ccacgtattg cgttccctgc aagatcataa
      121 tcaatccagt gaacctcttt cgtcaccggc ctctcgcctt tgctgtaccc taggagaatg
      181 tcagcaccag cagccaggat gatgattgcg ggaaaagtga tccaagtcca acgatagttt
      241 ctgatgccat atccgagaat ccaatagtcc accggaccaa tgatcaccag atagattacc
      301 acgagaactg ctacccatga gaatggaaca aacgatatgg tgggcaagac gtccaaggca
      361 ctccgcaatt gaccgctcac gtccgtgtaa ccgtaatgca tcagtgtccg cggtggattg
      421 ccggccacat catcctgggc cacagagtag ccaacaagcc ggtctatcag gcgatcaaac
      481 tgagtccatt cgaccaaagc gcggtgcgtc aaatccgtag taaaaaacac aatcgttcca
      541 aacccgtact gccgccggat gaccaagggg atattccctt cacttgccgt cacaatcccc
      601 tcaaaattcg taaactgaag agcctgaagc ggcggatcac tgcggcgcac tacaatcggc
      661 cgaggactat ccacatacgt ttcgatgcct gcacattttc tcaggggaat ggcattctgc
      721 cacgtaccgg gcgcgaccca cgaaaggacg ccaccgtcac tgacgaccgt cttagcagtc
      781 gggccaaagc tagccacgac tttgccgccc tcacgaaccc attcctgaag ggcaagcatt
      841 tgcttgccgg atcgctgcaa cctgccaata aactccttgt catcaccgat ggccaaaacg
      901 acggtacgga ccgactggta accgaggaag tgcgtcggca gccgttcggg gttcttgacc
      961 caaacccaat gtcctttctc gttgcgtctt cccatgaggg cctggggtgg ttgggatggg
     1021 cctacccaaa gaaataccgg agtccttgcc gctattgccg ttggaaagga tgcggtgccg
     1081 gcctgaaaga tcttttcgac cgaaggccca ccctcggctt cccatcgcac tgtagcctgg
     1141 gcccgcggtc gcccaatctg gaccagcatt tcgacctctc gcgttgcctg agcaggcagg
     1201 gtaacggtct ccataacgta agagcatggc ccaccttcgt cgtccggcac agttacaaca
     1261 atcgtgccgg aggcctcccc ggttcccccg cggagctcaa cccacacagg cgtccacagg
     1321 ccgagtttgt agaccccttc gacgcccaca cgcaaattga cgatctccgg ggcggtggtt
     1381 tctccatcag cacacaccgc ggcgatcgag gagaaccaga cgagaagctc taaaaagagg
     1441 atgagggctt gtttcatgga cttggaaaat gccgtttgta cggtcgctgt ttgttcacgc
     1501 cagtaatcca tcgatccgcg tcgctcacat tttcaattcc aaaacacctc gttgacacat
     1561 ccatccatct catctcagcc acagtcacct agggcttacc cgcgtcagga catacacaca
     1621 cgaaccgccc acatcccggg caaccctgac catatttctt ccgaatcgct gcggtgaggt
     1681 cgacatttga tacgttggca atcgtcgtta accaagcgac aacgtcggcg aattcctcag
     1741 ccttttcctc ggcagtcccg ctccgcaggg cctcggccaa ttcacccagt tcagcgacta
     1801 gccacatgaa ggtaccttcg acaccgcggg ccacgtcctt ctcaaaatac attttacgga
     1861 ttaggcgttg aaagtccgaa aacgtgatgt cacgatcgtc cgtctcctgt cggacggtct
     1921 cgtgggtttc gctcatcttt agtctccaca actcaggcgg ccgatttgct cagcacactc
     1981 ctctacaacg atccctccaa gagtcgttca atcaccgcaa ccaggccgtc ctcctcctgg
     2041 ggtggggcaa cccagtgggc cacctttttg acctcgtcgg gcgcatttcc catggcaacc
     2101 ccaaagccgg cttcacgaat catggaaatg tcgttcacgt catccccgac cgcgcagatc
     2161 tctcgtggcg tgacacctct ttgagcggcc aact
//
LOCUS       DAOPTR010000711         2194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39261_length_2194_cov_2.641421, whole genome shotgun sequence.
ACCESSION   DAOPTR010000711 DAOPTR010000000
VERSION     DAOPTR010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39261_length_2194_cov_2.641421"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PKI05_12425"
     CDS             <1..394
                     /locus_tag="PKI05_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53049.1"
                     /translation="QAFDYVHAYSLKGGRDVAQLKGSDGDDTFVGWPEWARLSGSNYF
                     LRVKFFEEIQVDPGAGFDSAILYDSPGADTLVATATSLTLSGDFGSISYLYQLQNFEK
                     VQAKSRGGTNTKSVDPAALTYLLLTGSW"
     gene            883..1113
                     /locus_tag="PKI05_12430"
     CDS             883..1113
                     /locus_tag="PKI05_12430"
                     /inference="COORDINATES: protein motif:HMM:NF022596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HOA53050.1"
                     /translation="MEFTSVGKFLVLIGVFLVIIGGLIWLLGGLPLHLGRLPGDIHIR
                     GERWSLHIPLATCIVLSILLTLVMNIAIRLFR"
     gene            1295..>2194
                     /locus_tag="PKI05_12435"
     CDS             1295..>2194
                     /locus_tag="PKI05_12435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA53051.1"
                     /translation="MSTQRTWGGGEEQARLIVRGLTARACDVLTAAPADSALASLLRQ
                     LGFPVFSLPHRARGPSAWLALRRAMRWFRPHVLFMNDPHAVIHGLLASLGLPVARIGA
                     RRTVFRLRSGWLYRQMLDGVVCSSLAAAQACRQAGILSQRISIIYDGVDLGRIRQANP
                     IRGRQVLTELARNCGGAPPSGSDQGDPESVHSGNGSPGTRFIVHVGKLTPAKGQDDLI
                     TAFAGLAKDHSDVAVVLIGDGEWRSHLEARMATLGLSNRVFFPGFRTDALDIVAAADV
                     FVFPSREEGLGGALIEALLLGVPV"
BASE COUNT          408 a          612 c          655 g          519 t
ORIGIN      
        1 ccaggctttc gattatgtcc acgcctattc cctgaagggt ggtcgggatg tcgcccaact
       61 caaaggctcc gacggcgatg acacgtttgt tggttggcca gaatgggcaa ggctttccgg
      121 gagtaactat ttcctgcgag tgaaattctt cgaagagatc caggtcgatc cgggggccgg
      181 gttcgactca gctatcttgt acgattctcc gggtgccgac actctggtgg ccacggcgac
      241 cagtctaacc ttgtccggtg attttggctc gatcagctat ctgtaccaat tgcagaattt
      301 tgaaaaagtt caagccaaat cccgcggggg aaccaacacc aagtccgtcg atccggctgc
      361 cctgacctac ctcctcctga ccggaagctg gtagtgtgcc cctcataaag ggtgggacag
      421 gccaatggtt agcagaatcc caacccgcac cggcgcaccg ctacactgac ccaacccacg
      481 gtcagtgtta cggtgtgccg tttttttttg gatgccttat cctgacggtg ttatccaaat
      541 gattcatgga aaacacccgt ggttggaaaa gctccctggc atcaagctcg cgggagcgcg
      601 tcacgagtac cacggaatcc ccttttctcg gcgcttcctt ttgacgtcag tgcgcataga
      661 atgacaaaag attgtgtgga gtgattatca tttcgcagta aagagcctat gccaaagttc
      721 cctctccctc tgggagaggg ttggggtgag ggcaagcgac cgttggagtc gatcgagaaa
      781 accggtagcg attcgtgcca cgctcaaccc cggacccttt cgccgaacgg gagaggggag
      841 gttgtgggat aggctcaaag gtatcgggga acgagaaagg ctatggaatt cacctcggtg
      901 ggtaaattcc tggttctgat cggggtgttc ctggtgatca ttggcggcct gatctggttg
      961 ctggggggcc tgccactgca cctgggacgc ctacccggcg atattcacat ccgcggtgag
     1021 cgctggtccc tccacatccc cttagccacg tgcattgtcc tgagcatact cctgacgttg
     1081 gtgatgaata ttgccattcg attgttccgt taaccttctt aggagcgata tcgccgggca
     1141 acacatatca ccggggcaga ggagaagggc tcgactccct agggtggaac gtggctccca
     1201 tatgtcgagc cttttggttt tgcgttggtg atctatgtta gccaaccgtt atgcaaatcc
     1261 taccggggca cgaccacgct tgcgggttct tttgttgtct actcaaagaa cctggggcgg
     1321 aggcgaggag caagctcgac ttattgttcg gggattgacc gcgagggcgt gtgacgtctt
     1381 gacggcggcc ccggccgata gcgccctggc gagccttttg cgccaattgg gcttcccggt
     1441 gttttccctt cctcatcggg cacgtggacc gtcggcgtgg ctagctctcc ggcgggcgat
     1501 gcgctggttc cggccgcacg tcctttttat gaacgacccc catgccgtca ttcacggcct
     1561 tttggccagt ttgggcttac ctgtcgcccg aattggggcc cgtcgtaccg tgtttcgtct
     1621 ccgctcgggc tggttgtacc gccaaatgct ggacggtgtg gtttgtagct ccttggcggc
     1681 cgcccaggcc tgtcgtcaag ccgggattct ctcccaacga atctcgatca tctatgacgg
     1741 ggtggattta ggccgaatcc gtcaggcaaa tcccattcgt gggcgacaag tcctcactga
     1801 gctcgcccga aattgcgggg gagctccgcc gtcgggaagc gaccagggag atcctgaaag
     1861 cgtgcattcg ggcaacggct cccccggaac acggttcatt gttcatgtgg ggaagctaac
     1921 ccccgccaaa ggtcaggacg accttatcac cgcgtttgcc ggactagcca aagaccattc
     1981 cgatgtggcc gtggtcctta tcggagacgg tgagtggcgc tcacatcttg aggcacgaat
     2041 ggcaacgctg gggctgagca accgcgtgtt tttccccggt tttcggacgg atgcccttga
     2101 catcgtggcc gccgccgatg tgtttgtctt cccttcgcgg gaggaaggct tgggtggggc
     2161 cttaatcgag gccctccttc tgggtgtacc ggtg
//
LOCUS       DAOPTR010000712         2192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39307_length_2192_cov_3.628451, whole genome shotgun sequence.
ACCESSION   DAOPTR010000712 DAOPTR010000000
VERSION     DAOPTR010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39307_length_2192_cov_3.628451"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="PKI05_12440"
     CDS             complement(<1..1349)
                     /locus_tag="PKI05_12440"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HOA53052.1"
                     /translation="MKAVHSQHVSLQGAAFWKAENKGLAEPRGSKALGESAERSSKHN
                     RQIAMAIPYWEQWREAAHQIKRYAIDHLDDLLVQFEKNLIARGCTVLWAKDAAEANQH
                     LLNIVKEHNVKTVVKSKSMVSEEIEVNHVLASVGVKALETDLGEFLVQLAGQRPTHIV
                     TPALHLSASDVGRLFAEKLGEPYTAEHEKLTDIARRHLRQEYLRADMGISGCNFGLAD
                     TGAICIVENEGNAGLSISAPPVHVALIGIEKVIPSIEYLPQFLNLLGRSGTGQKLTTY
                     THIVHGATPGRKFYVILIDNGRSNVLKDPAARTALFCIRCGACLNACPVYRRVGGWAY
                     GWVYPGPIGSILTPHLVGFDKAGKLPFASSLCGACGEVCPVKIDIPHQLVHLRHRAVN
                     TRSPVRSPLERLIWNAWAWAMRGPLRYQLTMGFVRLGVRLAKFLPFHPAQLGAWTRGR
                     "
     gene            complement(1402..2175)
                     /locus_tag="PKI05_12445"
     CDS             complement(1402..2175)
                     /locus_tag="PKI05_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOA53053.1"
                     /translation="MVRIGENGLPQWDYPTYERGEKVALFIPCYIDQFYPQIAQATAE
                     ILHRLGIPLVFPSEQTCCGQPAFNSGYWEEARRVIRHFVKVFRPYRYIVCPSGSCSAM
                     CRVFFGQADPDPATTEVGSRVFELTEFLVNILGVTDLGARFPHKVTMHSGCHGRRELG
                     IFEQPISLLKNVKDLVYCELPNIEECCGFGGTFSVKMPGTSIAMGETKAANILRSGAD
                     VVTSVDISCLMHVGGIMRRKEEMRHIRVMHIAEILNTRE"
BASE COUNT          504 a          639 c          579 g          470 t
ORIGIN      
        1 cgcccccgcg tccaggcccc caattgtgct gggtgaaatg gcagaaactt ggccagtctc
       61 acaccaaggc ggacaaaccc catggtcaac tgataacgga gcggtcctct catggcccag
      121 gcccaggcgt tccaaattaa acgttctaac ggggaccgaa ccggcgatcg ggtattcacc
      181 gcccgatgac gaaggtgcac aagctgatgg ggaatgtcaa ttttgaccgg gcagacctca
      241 ccgcaagcac cgcacaaaga ggatgcgaag gggagtttgc ccgccttgtc aaaacccacc
      301 aggtgcggcg tgaggatact gccaattggc cccggataga cccaaccata ggcccaaccg
      361 cccacacgac gataaacagg acacgcattc aaacaggcgc cgcatcggat gcagaacagg
      421 gccgtgcggg ctgccgggtc cttcaaaacg ttggaacgtc cgttgtcaat gagaatcacg
      481 taaaactttc tgcccggcgt ggccccgtgc acaatgtggg tgtaggtcgt caatttctgg
      541 cccgtgccac tgcgaccgag caggttcaaa aactgtggca gatactcaat actggggatg
      601 accttctcaa tgccaatcaa ggcaacatga accggcggag cggaaatgga cagaccggca
      661 ttaccctcat tttccacgat gcaaatggcg ccggtatcgg ccagtccgaa attgcatcca
      721 gagattccca tgtcggcccg caggtattcc tgtcgcagat ggcggcgggc aatatcggtc
      781 agcttttcat gctcggcggt ataaggttct cccagttttt ctgcgaatag tcggcccaca
      841 tcactggccg agaggtgaag cgccggcgtc acgatatggg tgggacgctg cccggcaagt
      901 tggacgagaa actcgcccaa gtccgtctcc agggccttga ccccaaccga ggccaagacg
      961 tgatttacct cgatttcctc gctgaccatg gacttcgatt taaccacggt cttcacgtta
     1021 tgttctttga cgatgttaag cagatgctga ttggcctccg cggcgtcttt ggcccaaagg
     1081 acggtgcatc cccgcgcgat caggttcttt tcgaactgaa ccagcagatc atcgagatga
     1141 tcaatcgcgt agcgtttgat ttgatgagcc gcttctcgcc attgttccca ataagggatg
     1201 gccatcgcga tctggcgatt gtgtttgctt gatcgctcgg ccgactctcc caaggcctta
     1261 gacccgcgtg gttctgcaag gcccttgttt tccgccttcc agaaggcggc cccctgaagc
     1321 gacacatgtt gggaatgaac cgctttcatc gtcgctcgcc tttttctcca ttgaatctgc
     1381 cgatcttccg gcaggtaatc tttattctcg tgtattcaga atctctgcaa tatgcatcac
     1441 gcggatgtgg cgcatttctt ctttccgccg catgatgcca cctacgtgca tcaggcaact
     1501 aatatccacg gacgtgacaa cgtcggcccc agagcgcaag atgttggccg cttttgtctc
     1561 ccccatagca atcgaggtcc ccggcatttt gacgctgaac gtaccgccga agccacaaca
     1621 ttcctcgata ttgggtaatt cgcagtacac gaggtccttg acgttcttaa ggaggcttat
     1681 cggttgttcg aaaattccta gctcacgccg accatgacat ccgctatgca tcgtcacctt
     1741 gtggggaaat cgggcaccga ggtccgtcac ccccaggata ttcacaagga actcggtcaa
     1801 ttcgaagacg cgggaaccga cctccgtggt ggccggatcg ggatccgctt gtccaaaaaa
     1861 gacgcggcac atcgcggagc acgaacccga cgggcagacg atgtaccgat acggtcggaa
     1921 gaccttgaca aagtgccgaa tgactcgacg ggcttcctcc cagtagcccg agttgaatgc
     1981 cggttgcccg cagcaggtct gttcactggg aaacactaag ggaatcccca aacgatgaag
     2041 aatctccgcg gtggcctggg caatttgcgg atagaactga tcgatgtaac agggaatgaa
     2101 aagagctacc ttttccccgc gctcataagt tggataatcc cactgcggca aaccattctc
     2161 gccaattcgg accataagcc caatccattc tt
//
LOCUS       DAOPTR010000713         2192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39310_length_2192_cov_3.109499, whole genome shotgun sequence.
ACCESSION   DAOPTR010000713 DAOPTR010000000
VERSION     DAOPTR010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39310_length_2192_cov_3.109499"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..1720)
                     /gene="rny"
                     /locus_tag="PKI05_12450"
     CDS             complement(185..1720)
                     /gene="rny"
                     /locus_tag="PKI05_12450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOA53054.1"
                     /translation="MAILCVLVAGLTFAVIRLLDYLRRKDAESEARRIIEQAERDAED
                     LKRRVQLELREKDLQERAAREREFQKIREELHQRERALEKREDALEEQASQLRRQEQI
                     VETTQRRLAERLEEVNRRKEELQKLLDLQRQVLHEVSGLSREEATKRLLDLLESQLQQ
                     EVGALVLKYEQKLQETCQQKAREILLTALQRYAAAHTAETTTSTVDIPNDEMKGRIIG
                     REGRNIRAFEKATGVDVIIDDTPGVVIVSAFDTVRREVARIALNKLIADGRIHPSRIE
                     EIVAETQQELDRFIQQQGEAACQEVDVHGLHERLIYLLGRLHFRTSYSQNVLRHSVEV
                     AFLAGMIADEMGLDGALARRCGLLHDIGKAADHEAEGGHPKIGADLLRRYGEPPEVIH
                     AAAAHHEDMRIENPYTVIVTAADACSASRPGARRETLERYIKRMAELESIALGFPGVE
                     QAYAIQAGRELRVMVNARETTDEIAAKICRDIARAFEERLTYPGEIKVTVLREIRCTE
                     IAR"
BASE COUNT          438 a          665 c          591 g          498 t
ORIGIN      
        1 atcgagtcgg caatggcgtc ttagcaaggg aagtgcccgg gtgacgatgc ggcgacccgg
       61 cttgccgaca acatctccta ccagcaacaa tcgcacgaaa tagacctttc tctggctgcg
      121 ggctacgggg cgaaaacccg gcgaaccgcc cctgtgatgg cccgtactct gtttccccaa
      181 tagctcagcg agcgatctca gtacagcgaa tctcgcggag gacggtaacc ttgatttctc
      241 caggataggt taatcgttcc tcaaaggcgc gtgcaatatc gcggcagatt tttgccgcga
      301 tttcgtccgt cgtctcgcga gcattgacca taacgcggag ctctcgccct gcctgaatcg
      361 cataggcctg ttccacacct ggaaagccca gcgcgatact ttcgagttcc gccatacgtt
      421 tgatgtagcg ctccaaagtc tcgcggcgag ctccggggcg tgaggcactg caagcgtccg
      481 ctgccgtaac aatgaccgtg taggggttct cgatacgcat gtcctcgtga tgggcggcag
      541 ccgcgtgaat cacctcgggc ggttcgccgt aacgtcgcaa aaggtcggcc ccgatcttcg
      601 gatgtcctcc ctcggcctcg tgatcggccg ccttaccgat atcgtgaagg aggccgcatc
      661 gccgggccaa ggcaccgtcc aggcccatct cgtccgcgat catccctgcg aggaacgcta
      721 cctccacgga atgccgcaac acgttttggc tgtagctggt ccggaaatgc agccttccca
      781 aaagataaat gagccgttcg tgtagtccat ggacatcaac ttcctgacag gcggcctctc
      841 cttgttgttg gataaaccgg tccaattcct gctgcgtttc ggcaacgatc tcttcgattc
      901 gcgacgggtg aatccgtcca tcggcaatga gtttgttcaa agcgattcgg gcgacttctc
      961 gacggaccgt gtcaaaagcg ctgacaatta ccacccctgg tgtatcgtca atgatgacat
     1021 cgaccccggt ggccttttcg aatgcccgaa tgttacggcc ttcgcgtccg atgatccggc
     1081 ctttcatttc atcgttggga atgtcaacgg tgctggtggt tgtctccgct gtgtgggcgg
     1141 cagcataccg ttgaagggcg gtaagcagga tctcacgagc tttttgctga caagtttcct
     1201 ggagcttctg ttcgtacttt aggaccagtg caccgacctc ctgttgcagt tgagactcga
     1261 gaaggtccaa cagccgtttg gtggcctctt cccgcgagag tccgctgacc tcatggagga
     1321 cttgccgctg gaggtccaac agtttttgca actcttcctt gcgacgattg acctcctcca
     1381 atcgctccgc caagcgacgt tgtgtcgtct cgacgatttg ctcctggcgt cgcaattggc
     1441 tggcctgttc ttccaaggcg tcttcacgct tttcgagtgc ccgctcgcgt tggtgaagtt
     1501 cctctcgaat cttctgaaac tctctttccc gagcagcgcg ctcttggagg tctttctctc
     1561 gcagctccaa ctggactcgc cgtttcagat cctcggcatc ccgctctgcc tgctcaataa
     1621 tgcggcgggc ttctgattcg gcgtctttac gccgcaagta gtctaatagc cgtataaccg
     1681 cgaacgtcaa tcctgcgact aagacgcaca ggattgccat gaaaagaatc tgattttctc
     1741 caaacacagt tgcgccctct catatcttgc cgggcgaggg cacggattcc gaaaggattg
     1801 ggaagagacg ctcaccgggg aacgctcggc cggaagttga aaaacctcat cagccatgag
     1861 cgtcctagcc gcacgctagt ggctaaccca tgtgagcaca aaccgagtga gaagccgtcc
     1921 ccaccgcggg atgccaaagc caacggcttc gccccactgg cggaaggatg tgtcgaaccc
     1981 gtggccgagc atggtaagac ctccgttcct tccggcctga cattttcctc aattggcccc
     2041 aagagccttc gaaggagagg tacctcccca cgtctcttcg agaccaacgg ccagaagacc
     2101 atgctgcggt tccgcgatcg aatggcactc gatatcccga cgagtgaata tgcatcccag
     2161 aagtaccaag ccggctctag gtgaaccgct tg
//
LOCUS       DAOPTR010000714         2189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39375_length_2189_cov_3.608716, whole genome shotgun sequence.
ACCESSION   DAOPTR010000714 DAOPTR010000000
VERSION     DAOPTR010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39375_length_2189_cov_3.608716"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PKI05_12455"
     CDS             <1..282
                     /locus_tag="PKI05_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53055.1"
                     /translation="CPSDFNSYEVPGAPSCRDGIGSRNIVVGTYGNMPSGRMEQVYGC
                     QWCYIYFACEPEGPFLYGNRWTYHCSIVQRFDQGGQWCNMHSSGSMCPQ"
     gene            291..1454
                     /locus_tag="PKI05_12460"
     CDS             291..1454
                     /locus_tag="PKI05_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53056.1"
                     /translation="MGKKFMKPWAKGTDTTHVRLMVRFVFSCILLASFVGVIAKTGKC
                     MAETELEAQLLRAVSAAHDRLVQSTEHVAGELKIIRKEGDASREETIRFAKDGPKLRL
                     EYSRLQGPPQAGPEWAKKERSHIVREIFIHSGKRFYHLEGYLNENSGVLYYSDFEDAE
                     FRVRCNSDFLYWLTILYRFPETSVLDLLQRPIAKVETRPWREVPNALWITGKPMVSEE
                     GTETDWTVVLNPDLLYAAVFYEFQARNATGGVSLRTSCEIEPQRLGEDTLLPRRVLRR
                     AENPGKTPTEWVYQFNFTSVGQVDPSLFEEESLKTLLGTIARVEVLPDGRRVTAGVFH
                     NELPLRAKSPEQRVISRRSVTISVITVGSIIVLLLFVIGSRVKKGSKRLHMKE"
     gene            1523..>2189
                     /locus_tag="PKI05_12465"
     CDS             1523..>2189
                     /locus_tag="PKI05_12465"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOA53057.1"
                     /translation="MDFIYGTLFIWRLATLSCVILSAGCGRVSGAPGGIYASPAVVDA
                     GKVNLAKQTEVSGDFRVVNSTSRIVKIVDTEVSCGCTRLMLSATSIAPHSSIRVQLVA
                     DLSDGQVGRRTFKALLITDSKSVPAVALELACHVTAEKRAGSVPINLGTLGPGEAFEQ
                     IRSVVSGRAKALRVAAIDAPDEVRRHLTVEPRCIADRMELIIRGRAPEKPGSFTIRVD
                     LLAE"
BASE COUNT          509 a          489 c          629 g          562 t
ORIGIN      
        1 tgtccttcag atttcaactc ttatgaggta cccggtgcgc cgagttgccg cgatggcata
       61 ggttcacgca atattgtcgt cggtacctac gggaatatgc ccagcgggcg aatggagcag
      121 gtctatggtt gtcagtggtg ttacatttat tttgcttgcg aacccgaagg gccatttctg
      181 tacggcaaca gatggaccta ccattgttcg atcgtccaga ggtttgatca gggtggtcag
      241 tggtgcaaca tgcattcatc cggctctatg tgcccacagt gaacttgata ttgggtaaga
      301 aattcatgaa accttgggcg aaaggtacgg acacgactca cgttcggctt atggtacgat
      361 tcgttttctc gtgtatattg ctggcatcct ttgtaggagt tatcgccaag actggcaaat
      421 gcatggctga aaccgagttg gaggcacagt tgcttcgggc tgtttcagcc gcccatgaca
      481 gactggttca aagcacagaa catgttgctg gagagctcaa gataatccgc aaagagggag
      541 atgcgtcacg agaagaaacg atcagatttg caaaggacgg acctaaactt cgcttagaat
      601 attcgcggtt gcaggggcca ccgcaagctg ggccggagtg ggcaaaaaag gagcgatctc
      661 acatcgttcg cgaaattttt atccattccg gaaagaggtt ttatcatttg gagggttatt
      721 tgaatgaaaa cagcggggtc ctttattatt cggattttga agacgctgaa ttccgggtaa
      781 gatgtaattc tgactttctc tattggctta ccatacttta ccggtttcct gaaacgtctg
      841 tgcttgattt actccagcgt ccaattgcca aggtggagac cagaccctgg agggaggttc
      901 ccaatgccct ctggattact ggcaaaccga tggtttctga agaaggaact gaaaccgatt
      961 ggacagtggt actcaatccg gacctgctgt atgcggccgt cttttatgag tttcaagcca
     1021 ggaatgcaac ggggggtgtg tccctcagga cttcatgtga gattgaaccc cagcgattag
     1081 gcgaggacac cttgctgcct cggcgagtgc tgaggcgggc tgagaatcca ggcaagacac
     1141 cgacggaatg ggtgtatcaa ttcaatttca cttcagtagg tcaggtcgat ccgtcgctat
     1201 ttgaagaaga gtccttgaag acattgctcg ggactatcgc gcgggtggag gttcttcctg
     1261 atgggcggcg tgtaaccgca ggggtctttc acaatgaact tcctttacgg gctaaatcgc
     1321 ctgagcagag agttatctct cgccgttcgg tgacaatatc tgtcattacg gttgggagca
     1381 ttatagtgct tctgttattc gtgatcggtt caagagtgaa aaaaggctcg aagcgtttac
     1441 acatgaaaga atgattttgg cataacgacc tagatgcgag gcacatactc cgagtaactc
     1501 tggctgtata atgaatatga aaatggactt catatacggg acactgttta tctggcgctt
     1561 agcgacgctg agctgtgtga ttctgtcggc agggtgtgga cgtgtatcgg gagctcccgg
     1621 tggcatctat gccagcccgg cagttgtcga tgctggcaag gtgaatttgg cgaaacagac
     1681 ggaagtttcc ggggatttca gggtcgtcaa ttcgacctcc cggattgtga aaatcgtgga
     1741 cacagaggtc tcctgcggtt gcacgcgact gatgttgtcg gcgacgagca tagccccgca
     1801 ttcctctatc agggtgcaac tcgtggcgga tctttcggat ggacaagtcg gtcggcgaac
     1861 gtttaaagca ctcctgatta ccgattctaa gtctgtaccg gcagttgctt tggagcttgc
     1921 atgtcatgtc acggcagaga aacgggccgg ttctgttccg attaacttgg ggaccttggg
     1981 gcctggtgag gcatttgaac aaattcggag tgttgtttct ggccgggcca aagctcttcg
     2041 cgttgccgca atcgacgcac ccgacgaagt ccgcaggcat ctcacggtag agcctagatg
     2101 catcgcggac agaatggagc tgatcattcg gggacgtgcc cccgagaaac cggggagctt
     2161 tacgattcgt gtcgatttat tggcagaag
//
LOCUS       DAOPTR010000715         2184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39481_length_2184_cov_2.837482, whole genome shotgun sequence.
ACCESSION   DAOPTR010000715 DAOPTR010000000
VERSION     DAOPTR010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39481_length_2184_cov_2.837482"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PKI05_12470"
     CDS             <1..773
                     /locus_tag="PKI05_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOA53058.1"
                     /translation="HCRLDFIVVNAENAAGGSGLTPELFYEIVEAGANCITMGDHIYS
                     RRQLEAVLRNDSRIVRPANYPPEAPGREFTILTAANQEPVAVISLLGRVFMRPVDCYL
                     QAADRVLDKLSSLVKVILIDFHAEATSDKQILGRYLDGRVSAVLGTHTHVPTADEQIL
                     PGGTAFQCDVGMTGPHESIIGRRIDRVLYTTRTFEPTPFEVATEDVRLNGTIVEVDPL
                     TGKSLGIWRLSLRESDLTETILSQVAKSVILPPARRTE"
     gene            1361..2005
                     /locus_tag="PKI05_12475"
     CDS             1361..2005
                     /locus_tag="PKI05_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53059.1"
                     /translation="MIALIRKLLALCLCVLPCVGLVGCSALATAMWITGASDVKPEYD
                     GFKDKKVAVVARPVVALTYRDAHVDKELARRIQLILTNRVRKAQWIDQQKVEKWLDEH
                     EWETFPEVGKGVGADLVIGVEIEDFSLYKGQTVYQGTATLRVAVYDCATNQKVYEKSL
                     SNINYPPNSVVPTSEVPESVFRREFLSVLAEAVARLFHSYDPHEFWALDTRTLR"
BASE COUNT          481 a          580 c          639 g          484 t
ORIGIN      
        1 gccattgccg actcgatttt atcgttgtca atgcggaaaa cgcggctggg ggatcggggc
       61 tgacacccga acttttctat gaaatcgtgg aggccggggc taattgtatc acgatgggag
      121 accatatcta ttcgcgacgg caattggagg ccgtgctccg caatgattca cggattgtgc
      181 gccctgcaaa ctacccccct gaagccccgg ggcgagaatt cacgatcctc acggccgcca
      241 accaagaacc ggtggccgtc atcagtcttc tgggtcgcgt cttcatgcga ccggtcgatt
      301 gctacctcca ggccgccgac cgggtccttg ataagctttc gagccttgtc aaggttatcc
      361 tgatagattt tcacgccgaa gcgaccagcg ataagcagat tttgggtcga tacttggatg
      421 gtcgagtctc ggccgtcctg ggaacgcata cccatgtccc cacggctgac gaacagattc
      481 tgccgggtgg tacggcgttc caatgcgatg tagggatgac aggcccccac gaaagtatca
      541 ttgggcgaag gatcgaccgc gtcctttaca ccacgaggac atttgaaccg acaccttttg
      601 aggttgctac agaagatgtt cggctgaacg ggacgattgt cgaagttgat ccgctgactg
      661 gtaagtcgct tggtatttgg cgactttccc ttcgggaatc tgacctcaca gaaacgatct
      721 tgtcgcaagt cgcgaaaagc gttatcctac ctcccgcgag aaggacggag tgaatgcgtc
      781 tccgtacggt tttgcgccaa acttcgtcgg cacagccctg tgatatgtgt agcccacact
      841 acccgggcga tgagataagg gtaaccgctc gctggccatg gacacaacga ctggaaatcg
      901 gggcgctagg ccttggattg gctggtttat tgttcgtggc ccgatggtta caaccagccg
      961 cagaaggatt tggaacgcac cagcagttgg ggctaccgcc gtgcaccatg gtggtggttt
     1021 ttggcaaacg ctgcccgacc tgcggtatta cgacagcctg ggcctattgg acgcgaggca
     1081 aggtccggga agcgttgagc tcgagtgttt cgggaacgtt gttggcggtg ctggccatgg
     1141 tgatggcacc ctggctgata atctccgcct atcgcgggca atgggcatgg tgggccccaa
     1201 agccaggcac cgttacctta gcttttgttg tcctgtggat aggtttgcta tgtgagtggt
     1261 tagtgagact ttggtgcaac tagaccggca gtcggccatg ggttaaaaga ccccatagcg
     1321 ggattcacgg acgaataggt agagttagca aggaatggct atgattgctc ttatccggaa
     1381 gttactcgct ctctgtttat gcgtgctccc ttgtgtgggc cttgtcggat gcagtgccct
     1441 ggctacagcg atgtggatta caggcgcttc ggacgtcaaa ccggagtatg acggcttcaa
     1501 ggacaagaaa gtggcagttg tggcacgccc cgtggtcgcg ctcacctacc gtgatgctca
     1561 cgtcgataaa gaactcgcgc gaagaattca gttgatcctc accaaccgcg tacgaaaggc
     1621 ccaatggatt gaccagcaaa aggtcgaaaa gtggctcgac gagcatgagt gggaaacctt
     1681 tcccgaggtg gggaaaggcg ttggcgccga cctggtgatt ggggtcgaaa tcgaagattt
     1741 cagtctgtac aagggacaga cagtttacca aggaacagcc acacttcggg tagctgttta
     1801 cgattgcgcc acgaaccaaa aggtttacga aaagtcgctc agcaatatta actatccacc
     1861 caactcggtc gtgcccacat cagaggtgcc ggagtcggtt ttccgccgcg agttcctctc
     1921 tgtcctggcg gaggccgtcg cgagattgtt ccattcttac gacccccacg aattctgggc
     1981 cctggataca agaaccctac gataaaaagg cagcagcgct taatgggaat tcggcggccc
     2041 gcgcggccgc gtcatgtcgt catgacttgc agcattcaga gcctatccca aaatcccctc
     2101 tccctccggg agagggttgg cgtgagggca agcgatcgtc ggagtcgatc caaaaaacca
     2161 gcagcgattc gagccaccct cacc
//
LOCUS       DAOPTR010000716         2184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39482_length_2184_cov_2.778300, whole genome shotgun sequence.
ACCESSION   DAOPTR010000716 DAOPTR010000000
VERSION     DAOPTR010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39482_length_2184_cov_2.778300"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PKI05_12480"
     CDS             <1..562
                     /locus_tag="PKI05_12480"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOA53060.1"
                     /translation="LKTGRYLWNAGIFVWTISAFRHALARHAPLLHEGLCQIERSLEE
                     GFDAVREKFLTLERRSIDYALMEKAENIVVVPGDFGWDDVGTWAALERIYDKDENGNV
                     IKGEAVAIEAGGNIIDNSLGDKLVVAFGVKDLVIVNSEDVVLVADKKRSGSLKKVTEV
                     LRNRKMRELLERERGREVPLERKGLL"
     gene            726..1013
                     /locus_tag="PKI05_12485"
     CDS             726..1013
                     /locus_tag="PKI05_12485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HOA53061.1"
                     /translation="MRTLQIKVVQVKGYCPVYNPGDQFAILEGYKLRVERVLCMHSLA
                     SILPYYVALSRGVSPKELGLGDGATAYVQCLDPCEYTGGGTVVFGITGGGL"
     gene            1010..>2184
                     /locus_tag="PKI05_12490"
     CDS             1010..>2184
                     /locus_tag="PKI05_12490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOA53062.1"
                     /translation="MNSVCVLGLGYIGLPTASILATHGFTVIGVDVNEKVIARLKEGK
                     PHIQEPGLRTIVEAAVKSGNLILRTKPEEADAFIIAVPTPVTPEKKADLSFVKAAAES
                     IVPVLRPGNLVVLESTCPPGTAENFLTPILARSGMRIGQEVFVAYCPERVLPGKILKE
                     IVENDRVIGGINPESAAKAKELYGAFVEGKLFLTDATTAEMVKLIENTYRDVNIALAN
                     ELAIVCEELGINAWEVIELANRHPRVSLHNPGPGVGGHCLPVDPWFVVEKFPTQARLI
                     RLSREINDRQPYRVFELVAEMTRNIPGPKVAVLGVTYKGNVDDIRESPATVLIELLKE
                     RGYSVAAHDPYVKEFEHELVSLEEAFRGADCAVAMADHDQYRYLDPNTLGKLMRNRQ"
BASE COUNT          514 a          518 c          674 g          478 t
ORIGIN      
        1 tctcaagacc ggccggtacc tctggaatgc ggggattttt gtctggacca tctccgcctt
       61 ccggcacgcc ttggccaggc atgcccctct tctccatgag ggtttgtgcc agatcgaaag
      121 atcgttggaa gaaggatttg atgccgtgag ggagaaattt cttaccctgg agcggcggtc
      181 catcgactat gccctcatgg agaaggcgga gaacatcgtg gtcgtgcccg gggacttcgg
      241 ctgggacgat gtggggacct gggcggcttt ggagcggatc tacgacaaag acgagaacgg
      301 caacgtgatc aagggcgagg ccgtggccat cgaggcgggc gggaatatta tcgataattc
      361 tttaggggac aagctcgtgg tggcctttgg ggttaaggac ctcgtgatcg tgaattccga
      421 agatgtggtg ctggtggcgg ataagaagag gagcgggagt ttgaagaagg tgacggaggt
      481 attgagaaac cggaagatgc gggaactatt ggagagggaa agaggaaggg aagttcctct
      541 tgaaagaaaa gggttgcttt agtttttagt atggaattgt ctctcactaa aataaaaaat
      601 ctatgaagct tcataaaaac aatttcatat ttatatacac agccttgctt ggagaccgga
      661 cagcctaacc tgggacggct atgctgcgaa aaaaacgtca agacacttag agaggttctg
      721 ataatatgcg aacgttgcag atcaaagttg tccaggtcaa aggttattgt cctgtttaca
      781 atcccggcga tcagtttgca atcctggagg gctataagtt acgagtcgaa agagtgctct
      841 gtatgcattc tttggcttcg atccttccct attacgtggc cttgagtaga ggggtatcgc
      901 caaaggagct tggcctgggg gatggcgcga cagcatatgt ccagtgcctt gacccttgtg
      961 aatataccgg agggggcact gtggttttcg ggattacagg aggtggctta tgaatagcgt
     1021 ttgcgtatta ggcctcggtt acatcggcct gcccacggcc agtatcctcg ctacgcacgg
     1081 atttaccgtt atcggcgtgg atgtgaatga aaaggttatc gccaggctca aggaaggcaa
     1141 accccacatc caagaacccg ggctacggac gatagtcgaa gcggcggtca agtcgggaaa
     1201 cctgattctc cggaccaaac cggaggaggc cgatgccttc atcattgcgg tccctacgcc
     1261 ggtgacgccg gagaaaaaag cggacctgag ttttgtaaaa gccgcggcgg agagcatcgt
     1321 gcccgtcctg cggccgggaa atctggttgt attggaatca acgtgtccgc ccggaacggc
     1381 ggaaaacttt ctgaccccga ttctggcccg gtctgggatg aggatcggcc aggaggtgtt
     1441 cgtggcctac tgtccggaga gggttctccc ggggaagatt ttgaaggaaa tcgtggaaaa
     1501 cgaccgcgtc atcggcggaa tcaacccgga gtccgccgca aaggccaaag aactctacgg
     1561 cgcttttgta gaaggcaagc tatttctaac cgacgccacc acggcggaga tggtcaagct
     1621 catcgagaat acataccgcg acgttaacat cgccctggcc aacgagctcg ccatcgtctg
     1681 tgaggagttg ggcataaacg cctgggaggt catcgagctg gccaatcggc acccgcgggt
     1741 gagtctgcac aatcccggcc ccggggtggg cggccattgt cttccggtgg acccatggtt
     1801 cgtggtggag aagttcccta cccaggcgcg cctcattcgg ctgagtcggg agatcaacga
     1861 tcgacaaccg tatcgggtct ttgagcttgt tgccgaaatg acgagaaata ttcccggccc
     1921 gaaggtggcc gttttgggtg taacttataa aggtaatgtt gacgatatcc gggagagccc
     1981 ggcgacggtt ctcatcgagc tcctgaaaga acgcgggtac tccgtagccg cccacgatcc
     2041 ttatgtaaaa gagttcgagc atgaattggt cagcctggag gaagctttcc ggggggccga
     2101 ctgcgccgtg gcaatggccg accacgacca gtaccgttac ttggatccca atactctggg
     2161 taagcttatg cgcaacaggc agat
//
LOCUS       DAOPTR010000717         2184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39484_length_2184_cov_2.662283, whole genome shotgun sequence.
ACCESSION   DAOPTR010000717 DAOPTR010000000
VERSION     DAOPTR010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39484_length_2184_cov_2.662283"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PKI05_12495"
     CDS             <1..530
                     /locus_tag="PKI05_12495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913315.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOA53063.1"
                     /translation="ITDGQIYLQPYLFFAGQRPAMNVGISVSRVGGKAQIPAMKKVAG
                     GLRLDLAAFRELEAFAQLGTELDPATQARLDRGYRMTELLKQPQYQPMHVTDQVLVIY
                     AGTKGYLDPIPVTQVRNWEKEFLDFVHQNHRALWQHITDTKQLDSETEQKLVAVLEEF
                     NQQYLAKTKEAAATA"
     gene            640..1521
                     /gene="atpG"
                     /locus_tag="PKI05_12500"
     CDS             640..1521
                     /gene="atpG"
                     /locus_tag="PKI05_12500"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOA53064.1"
                     /translation="MAKARALERRRRSVRNIRKITRTMELISTARFKRAMDRAMAASA
                     YTQRLTNLLRHLVEAGTEIRHPLMQRHAETRRVLVLVLTSNRGLCGGFNSGVLRLALS
                     HLRSLRTKVEQTRIEVSGKRGISAFKYRRIPVDERYTHFDARPQFEDVERLAVRYWDA
                     FSSGDVDQVDVVYTKFESASRQYPVVETLLPLSSLDMEEENGLLHAVEYEFLPSPESI
                     LEEIVPTAYKMRLFRCFLESAAAEQIARMVAMKSATENADTMLANLSIAYNRARQTQI
                     TSEILEIVSGAEALEKQ"
     gene            1606..>2184
                     /locus_tag="PKI05_12505"
     CDS             1606..>2184
                     /locus_tag="PKI05_12505"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651908.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOA53065.1"
                     /translation="MNATATSGRPRVDVVREPNYGRITQVIGSTFDAEFPEHRLPAIY
                     NAVKIQEQVKGLSINLTGEVQQHLGGGRVRCVALGSTDGLVRGMKCLDTGGPVKVPVG
                     KGILGRVFNLLGEPIDDRGPVEAEDWWPIHREAPPVTELSTKVEIFETGIKVIDLLTP
                     FVRGGKAGLFGGAGLGKTVILTELIARIATSHG"
BASE COUNT          510 a          552 c          644 g          478 t
ORIGIN      
        1 ctatcacgga cggccaaatc tacctccagc cctacctatt ttttgccggt cagcggcccg
       61 ccatgaacgt tggtatttcg gtctctcggg tcggtggcaa ggcccagatt ccggctatga
      121 agaaagttgc gggtggtttg cgtctggact tggcggcatt ccgcgagctg gaagctttcg
      181 cccaactggg aacagaactc gatcccgcaa cacaagcccg ccttgatcgt ggatatcgga
      241 tgacggagct tttgaagcaa ccacaatacc agcccatgca cgtcacagac caggtgctgg
      301 tcatttatgc gggcacaaaa ggatatctgg atcctatccc ggtaacgcaa gttcgaaatt
      361 gggaaaagga atttttggac ttcgtacacc aaaatcatcg ggccctttgg cagcacatta
      421 ccgataccaa acaactcgat tcggaaacgg agcaaaaact ggtggctgtc ctggaggagt
      481 ttaaccagca gtatttggcc aagacaaagg aggcagcggc aaccgcatag tgtgcttgcc
      541 cgaaagtttc tcgacacaaa agatgatgct gtggtgaggg ttggcgacaa gtgagtttgg
      601 ggggcccggg gaacgtgata tcagaataga tacgcaggca tggcaaaggc aagagcatta
      661 gagcgaaggc gtcgttcggt ccggaatatc cgcaagatta cgcggacgat ggagctgatt
      721 tccaccgcgc gattcaagcg ggcgatggat cgcgccatgg ccgcctcggc ctatacgcaa
      781 cgactgacca acctgctgcg gcatcttgta gaggcgggga ccgagattcg ccatccactc
      841 atgcagcgac atgccgagac ccgtcgcgtg ctcgtcttgg tgttgacctc caatcgtggc
      901 ctgtgcggtg ggtttaactc cggtgtcctg cggctggccc tatcccacct gcggtcgctc
      961 cggaccaagg tcgagcaaac acggattgag gtttcaggca aacggggcat ttccgcgttt
     1021 aaataccggc gcattccggt cgatgagcgc tacacgcatt ttgatgcccg tccccaattt
     1081 gaggacgtag aaagactcgc ggtgcggtac tgggatgcgt tttcgagcgg ggacgttgac
     1141 caagtggatg ttgtttacac gaaattcgaa tcagccagcc gacaatatcc tgttgtcgaa
     1201 actttgttgc ctttaagcag tctcgatatg gaagaagaga atgggctgct tcacgccgtg
     1261 gaatatgaat ttttaccttc tccggagagc attttggaag agatcgtccc cacggcgtac
     1321 aaaatgcggt tgttccgatg ctttcttgag tctgctgcgg cagaacagat tgcccgcatg
     1381 gtcgccatga agtcggccac agaaaatgct gacaccatgc tggccaatct cagtattgcg
     1441 tataaccgag cccgacaaac acaaatcaca tcagagattc tggagatcgt cagtggcgcg
     1501 gaagctttgg agaaacaata agtggtgcgc ctcgttccgc gatcgtgaag cgacagcccg
     1561 atccattcga tcaatcaagt gactccttgt tgaggtaaga ccatgatgaa tgcaacggca
     1621 acatcaggac gtccgagagt ggatgtcgtt cgggagccca actatgggcg aatcacccag
     1681 gtgattggct caacgtttga cgccgagttt cccgaacatc gtttgccggc gatctacaat
     1741 gccgtcaaga ttcaggaaca ggttaagggg ctttccatca atttgacggg cgaagtgcag
     1801 caacacctgg gcggcggtcg tgtgcggtgc gtcgccttag gaagcacgga cggactggtg
     1861 cgggggatga aatgccttga cacgggtggg cccgtcaaag tgccggttgg taaagggatc
     1921 ctcggccgtg tgttcaacct gttgggcgaa cccattgacg atcgtgggcc ggtggaggct
     1981 gaggactggt ggcccattca ccgagaagcc ccgccggtga ctgagttgtc caccaaagta
     2041 gagattttcg aaaccggcat caaagttatt gatttgctaa caccctttgt gcggggcggg
     2101 aaagccgggc tctttggtgg tgccggtctg gggaagactg tcattctgac tgaactgatc
     2161 gcaagaattg ctacatcgca cggg
//
LOCUS       DAOPTR010000718         2183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39503_length_2183_cov_3.106673, whole genome shotgun sequence.
ACCESSION   DAOPTR010000718 DAOPTR010000000
VERSION     DAOPTR010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39503_length_2183_cov_3.106673"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1361
                     /locus_tag="PKI05_12510"
     CDS             <1..1361
                     /locus_tag="PKI05_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53066.1"
                     /translation="DDNYSEAIGLSVFAVSCQLGLENHESSALGANGMFKDMIARLLW
                     ACFMSFMLVGDRIAWATDYVTRATGSPSAYWSSRVLGRPTSTGKVIDSRPIVTSQVLQ
                     PDRATEYSIQTPRTRFISENIQPVPEMIPPGEPITPVEGVEDGEYLDSAFVPVEDNLL
                     SCGSGYCEGACYGCFPFGPWIQHLSVFAGAHGFKGTADLGRNGNFGLHEGLNYSAPLG
                     GPWNIGYQVGVQVVHSDFAGHQTLTWYGQDQITNASRAQMFFTAGLFRRPECDRWQWS
                     VLFDWMHDDYYVDSDLLQIRHEISFFLDDQREIGYMGMYGVKDDLLLLRDGNEQERRY
                     LDLSVLDRYAMFYRWHFCRGGEGRFWAGVSGYGDGLIGGDIVLPLDRSLALEANFAYL
                     APNTGTGADGQMDEHWSLSIGLVWYPGRPVDEALGDLFRSVQGVADNTSMMFHGRIRN
                     SP"
     gene            1737..>2183
                     /locus_tag="PKI05_12515"
     CDS             1737..>2183
                     /locus_tag="PKI05_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA53067.1"
                     /translation="MKAKATRRQFLGVTGVVGGVLSGVPMLIPASALGLTAEPAPSER
                     ITLGVIGIGPRATYDLQAMLKLPDVRCLAIADVQASRREAGKKLVDEFYGNQDCVTYR
                     DFRELLDRKDIDTVLVATGDRWHAPASIMAAEAGKDVYREKPCGITI"
BASE COUNT          441 a          571 c          652 g          519 t
ORIGIN      
        1 atgacgataa ctattccgaa gcgatcggat tgagtgtttt cgcggtgtca tgccaactcg
       61 gtttggagaa tcacgaatcg agtgctttgg gagccaatgg tatgttcaaa gacatgattg
      121 ctcgtctcct ctgggcgtgc tttatgagct ttatgctcgt gggagatcga atcgcctggg
      181 ctaccgatta tgtcacccgc gcgacgggtt ccccctcagc ctactggtcg agccgcgtgc
      241 tcggccgtcc cacatccact gggaaggtaa tcgactcccg tcccattgtg acaagccagg
      301 tgcttcagcc ggatcgggct accgagtatt caatccagac cccgcgtacc cggttcatat
      361 cggagaacat acagccggtg cccgagatga ttccgcctgg tgaacctatc acccctgtcg
      421 aaggtgtgga ggatggcgag tatctcgatt cggctttcgt ccccgtggag gataatcttc
      481 ttagctgtgg ttccggctat tgtgaagggg cgtgctacgg ctgttttcct tttggcccgt
      541 ggatccaaca cctcagcgtt tttgcaggtg cccacggctt taagggaacc gccgatttag
      601 gtcgaaacgg caacttcggc ctccatgagg gacttaacta ctcagcgcct ttgggtggcc
      661 cttggaatat tgggtatcag gtcggggtgc aggttgtcca cagtgacttc gccggacatc
      721 aaaccttgac atggtacggg caagatcaga tcactaatgc tagccgggcc cagatgttct
      781 tcaccgccgg tctctttcgc cgacccgagt gtgatcgttg gcaatggagc gtgctgttcg
      841 actggatgca tgatgactac tacgtcgatt ccgacttgct ccagatacgt cacgagatct
      901 cgtttttcct cgacgaccag cgcgagatcg gttatatggg catgtacggc gtcaaggacg
      961 acctactgtt actgcgtgat ggtaacgagc aggagagacg gtatctggat ttgtctgtcc
     1021 tcgatcggta cgccatgttt tatcggtggc acttttgtcg cggaggcgaa gggcggtttt
     1081 gggccggtgt ctccggatac ggcgatggcc tgatcggtgg cgatatcgtg ttgccgttgg
     1141 accggagctt ggctctcgag gcgaatttcg cctatttggc cccgaacacc ggtacaggag
     1201 ccgacgggca aatggacgaa cattggagct tatccattgg cctagtttgg taccccggac
     1261 ggccagtgga cgaggcactt ggcgacctat ttcggtcggt ccaaggcgtg gccgacaaca
     1321 caagcatgat gttccatgga agaatcagaa attccccctg agcaaccaca tgtcagtttg
     1381 aggctcccat tggctccgag ggtaccgacg attcggtgcc ctccttcttt tgcgctgtgc
     1441 taagagtcag tctccctctc cctccgggag agggtggggt gagggcaagc gatcgtcgga
     1501 gtcgatcgag aaaaccggca ggtattcgag ccacactcac ccccaatctc tctcccgcgg
     1561 aacgggcaag ggaagatagt gggacaggct ctaaatccat ggccacggct tgattgattt
     1621 gaccatcgct tgattccccc gattggccac agtaggctga ggacacacca agtatgggtt
     1681 tagttggacg tgtaccttac gagttgtggt gtcaaatttg aggaaaggaa ctgctgatga
     1741 aagccaaagc aactcggcgt cagtttcttg gggtcaccgg tgtcgtgggt ggggtcctca
     1801 gtggagttcc catgttaatt ccggccagcg cacttggact aactgctgaa cctgccccga
     1861 gtgaacgaat cactttgggg gtcattggca ttgggcctcg cgccacgtac gacctacaag
     1921 cgatgcttaa gttacccgat gtccgatgtc tggccatcgc cgatgtccag gcaagccgac
     1981 gggaggccgg caaaaagctg gttgatgagt tttatggtaa ccaggattgt gtgacctatc
     2041 gggacttccg cgagctactt gaccgcaagg acatcgatac cgtcttggta gccactggcg
     2101 accgctggca cgcgccggct tcgatcatgg cggcagaggc gggtaaggac gtgtataggg
     2161 agaagccatg cgggatcacg att
//
LOCUS       DAOPTR010000719         2183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39505_length_2183_cov_3.057801, whole genome shotgun sequence.
ACCESSION   DAOPTR010000719 DAOPTR010000000
VERSION     DAOPTR010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39505_length_2183_cov_3.057801"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PKI05_12520"
     CDS             complement(<1..292)
                     /locus_tag="PKI05_12520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOA53068.1"
                     /translation="MSVDTPAIETSHLTKVYGNEVIAVNDLTFEVPPGVIFGFLGPNG
                     AGKTTTLRLLLGLQRPTAGQAKVFGHPCGPDATAVRRLIGYLPTNPNLPGHLK"
     gene            872..>2183
                     /locus_tag="PKI05_12525"
     CDS             872..>2183
                     /locus_tag="PKI05_12525"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOA53069.1"
                     /translation="MNILPRFVAIIGIVLDILLITGCETAPSAAPPPGPPEVAVYTVE
                     TTKVRITTELPGRTASYLVSEIRPQVNGVILKRFFEEGSEVKAGDVLYQIDPAPYQAA
                     YDVALANLKTAQQAVEKARAALEASMAAQKEQEALFHLARTNRERYERMYEQLATTAM
                     ERDQAVTNAEVAEAALLAAKARVETDRKALAAAEAAVQQAEAALEAAKINLDYTQVKA
                     PISGRIGRSRVTEGAIVTAYQSTPLATIQQFDPIYVDIPQSTSQLYRLRRALQQGRLK
                     AEGTDKVKIILEDGTIYPHEATLKFRDVSVDPGTESVIVRVIVPNPEGELLPGMFVRA
                     SIELGEQQDAILVPQQAVQRDSRGEPFVWVVDDQNKVHVRSIVTDRAFGHRWLVLEGL
                     KAGERIVMEGMQRLKPDMVVNVVPWVEPSAAASQESSRGSELANG"
BASE COUNT          484 a          584 c          669 g          446 t
ORIGIN      
        1 gcttcaaatg ccctggcaga ttggggttgg tgggcaggta gccgatcaga cgccgcacgg
       61 ccgtagcgtc cggcccgcag ggatgcccaa acaccttggc ttggccggct gtgggccgtt
      121 gcaaaccgag aaggagtcgt aaggtggtcg tcttcccggc cccgttaggt ccaagaaacc
      181 cgaagatgac accgggcggt acctcaaacg tcaggtcgtt gaccgcgatg acctcattgc
      241 cataaacctt ggtcaggtga gaggtctcaa tggccggagt atccacactc atggatgtca
      301 ccatcgctta ataaatctcg gcttggtcgg gaggcggacg cgcctggtgc gaggcccatg
      361 gccgatcttg ataatattac acgccaatgg gcgtattcgt tacaggtgtc ctattctttg
      421 gccatgatat tcacaacgta gcggtgaaat caaaacctcc acccgcgtga ggtctgatag
      481 gaacaagaaa ggtgagccgc gagcatgcga gtcgccaggg caagctggcg gtgcttcccg
      541 attgaccgtg cgttaagcgt tctcataggg gcaactgatg gaagccacta cggatatcta
      601 acgtcacggg cccgcccatc ggcaggggga aggtgcacca tgtcgcattt gccaaagagg
      661 gtaccatttc aagctcgtcg taactatagt aaaagcgcgc gacctgcccg atgaaatcag
      721 attcccgccc agaacacgct aacacgagat cgcttcgggg gtttcttagg atgaacacga
      781 tgttgggtag gacgttcgtt ggtttattga cgggcggatg atgaatcgct tccgggagat
      841 cgagtgattc gccgcggcgt ggagtgtctt catgaacatc ctgccaagat ttgtagccat
      901 catcggaatt gttttggata ttctcttgat cacgggctgt gagacggcgc catccgcagc
      961 gccgccccca ggacctccgg aagttgccgt ttacaccgtg gagacgacca aggtccgcat
     1021 cacgacggag ttacccggtc gaacggcctc ctatttggtg tcggagattc gcccgcaggt
     1081 caacggggtg attttgaaac gcttttttga ggaaggctcg gaagtcaagg cgggagatgt
     1141 gttgtatcaa attgatccgg cgccttatca ggcggcttac gacgttgcgt tggccaatct
     1201 gaaaacggcc caacaagccg tggaaaaggc ccgggccgcc cttgaggcca gcatggcagc
     1261 gcaaaaagaa caagaggccc tttttcacct tgctcgcacg aatcgtgagc gctacgaacg
     1321 gatgtacgaa cagctagcga ccacggccat ggagcgtgat caagccgtga caaatgccga
     1381 ggtggccgag gccgcgttgc ttgcggccaa agcccgcgtc gaaaccgatc ggaaggccct
     1441 ggccgcagcg gaggctgccg tccagcaggc cgaggctgcg ctggaggcgg ccaagatcaa
     1501 tctcgactac acccaggtca aggcaccgat ctccggacgg attggacgat cccgagtcac
     1561 agagggggcc attgtgacgg cctatcaatc gacacctctt gccacgattc agcaatttga
     1621 ccccatttac gttgatatcc cgcaatcgac cagccaactg tatcgtctcc gtcgggcatt
     1681 gcagcagggg cgactgaagg ccgagggtac cgataaggtc aagatcatcc tcgaggatgg
     1741 caccatctat ccccatgagg caacactgaa atttcgcgat gtcagtgtcg atccgggcac
     1801 cgagtccgtg atcgtgcggg tgatcgttcc caatcccgag ggggaattac tcccggggat
     1861 gtttgtccgc gccagcattg agctaggcga gcagcaggat gcgattctcg tgccccaaca
     1921 agctgttcaa cgggactcgc gaggggagcc tttcgtgtgg gtggtggatg atcagaacaa
     1981 ggttcatgtc cgttcgatcg tcaccgatcg tgcctttggt catcgctggt tggtactaga
     2041 gggattaaag gcgggggagc gaatcgtgat ggaagggatg cagaggctca agccggatat
     2101 ggtcgtcaat gttgtgcctt gggtcgaacc atccgctgcg gcatcccagg aatcatcacg
     2161 agggtctgag ttagccaacg gga
//
LOCUS       DAOPTR010000720         2183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39506_length_2183_cov_3.048402, whole genome shotgun sequence.
ACCESSION   DAOPTR010000720 DAOPTR010000000
VERSION     DAOPTR010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39506_length_2183_cov_3.048402"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2183)
                     /locus_tag="PKI05_12530"
     CDS             complement(<1..>2183)
                     /locus_tag="PKI05_12530"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53070.1"
                     /translation="DSVPARVAREYGLLPLERKSDHLLVAVSEPLLPEQSDRLRFTLD
                     CPVRTVLAVKEHLLKAIAKWYGEEKDAQEAESPSTGEGKESEKGAVEFIQVEPEESQA
                     ATEVVIDPDSPEVTRAIQTIVGEGIRLGASRVALVSYHGVTKVGFRVEDGICSRNDLP
                     GELLAPIAARLIGMTNFYGTIKVVVRGTERRLKASCRTTPYGLALVIEFDKDESAIQA
                     ARERASLLSYPFTELEKPQIPADVLATIPRDIARTYYVLPLSVTNDVVAIAMAEPPGP
                     QLQDELRFALGRPFQVMLAPEGRLRAAIERFYGPAEPEAVTVLLAELARPMTTAEVAG
                     RLATWERPSPPQGHPAEAIFGYLRQMATEKLLELFEDIRRRPKLCIRSSKRWQLEVVI
                     PRADLFSVLPQAYRSYLENRLWLVREAIIARIFNIIVRHPEARHLAIAYALYVAACRL
                     AEGERNVPIDPVMLIDEWFNFLYCYALKTNPKIETNAALLNYLSNQSEELVRSIVGVT
                     RDPSLVQNIELAPRWLEALAQQTLVQDVIDETCGTAEAMLNLFVAEARHYRASHMLLL
                     PHPEYIEVAYRIQNRILSTVRVPACWFLPILSRLLANLVNLSEWQVTVAGKTIQAQVR
                     LHVAAEGLAALIEFMADSRQVEVAQMLAASTDCRFVRLHDVQVPEDILKLVDGGVARH
                     LCVLPIATDGETIILAVSQPPSSRHMQELQILLARPIQAVIAPED"
BASE COUNT          499 a          650 c          560 g          474 t
ORIGIN      
        1 tcgtcctctg gggcaatcac tgcttgaatg ggacgggcaa gaagaatctg tagttcttgc
       61 atatgccggg agcttggcgg ttgggacacg gcaaggataa tcgtctcgcc atctgtggca
      121 atgggcaaga cacaaagatg ccgggcgacg ccgccgtcga ccaacttcaa tatatcctct
      181 ggtacttgaa cgtcgtgcag tctcacgaat cgacaatctg tgctcgcagc tagcatctga
      241 gccacctcga cctgtcgcga gtcagccatg aattcgatca aggcggcgag gccctcggct
      301 gccacatgca accgtacttg ggcttgaatc gtctttccgg caactgtcac ctgccactcg
      361 ctgaggttca ccaaattggc aagcaacctt gagaggatcg gcaagaacca gcaggccggc
      421 acacgcacgg tggacaaaat gcgattctgg atccgataag ccacctcaat atactcagga
      481 tgcggaagaa gcaacatgtg ggatgcccgg taatggcgtg cttcagccac aaatagatta
      541 agcatcgcct ccgccgtccc gcaggtttcg tcaatgacat cctgcacaag cgtctgttgc
      601 gccaatgctt ccagccaccg gggggctaac tcgatgttct gaaccaacga tggatcgcga
      661 gtgacaccaa cgatactccg cactaattcc tccgactggt tcgaaagata gttaagcagg
      721 gcggcattcg tctcaatttt ggggttcgtt ttcaatgcat agcaatagag aaaattgaac
      781 cattcatcga tcaacatcac cgggtcgatg ggaacatttc gttcaccctc cgcaagtcga
      841 cacgccgcga cataaagggc ataagcaatc gccaggtggc gcgcctccgg atgtctcacg
      901 atgatattga agatccgcgc aataattgcc tctcgaacca gccaaaggcg attttccaga
      961 tagctgcgat atgcctgcgg taacactgaa aacagatccg cccgaggaat aacgacctca
     1021 agttgccagc gttttgaaga tcggatacag agtttcgggc gacgccggat atcctcaaac
     1081 aactccagta acttttctgt cgccatttgc cgcagatacc caaaaatggc ttctgcagga
     1141 tggccctgag gtggcgatgg tcgctcccac gtcgctagac gcccggctac ttccgcagtc
     1201 gtcatcggcc gtgccaactc ggcgaggagc actgttaccg cctccggttc cgccggtccg
     1261 tagaaccttt caatcgccgc gcggagccgt ccctccggtg ccagcatcac ttggaagggt
     1321 cgccccaatg caaatcgcaa ttcgtcctgc aactgtggac cgggtggctc cgccatggca
     1381 atcgcaacca cgtcgttggt caccgacaag ggcaaaacgt agtacgttcg cgcaatgtcg
     1441 cgcggaatcg tggccaagac gtctgctgga atctgaggct tttccagttc tgtaaacggg
     1501 taactcaaga ggctggccct ttcccgcgca gcctgaatcg ccgattcgtc tttatcaaac
     1561 tcgataacca gtgccaggcc ataaggggtg gtacgacaag acgccttcag ccgccgctca
     1621 gttccccgaa ccaccacctt gattgtcccg tagaaattcg tcatccctat gaggcgtgca
     1681 gcaatcggtg ccaataattc gccaggcagg tcatttcgtg aacagatgcc gtcttcgaca
     1741 cgaaagccga ccttggtgac accatgataa gacaccaggg cgacccggga tgcccccaac
     1801 cgaatccctt cccctacgat ggtctgaatt gcccgtgtta cctctggcga atccgggtcg
     1861 atcaccacct ccgttgccgc ttgtgattcc tcgggctcaa cctggatgaa ttccactgcc
     1921 cctttctccg actctttccc ctcgccggtc gatggagact cggcctcctg ggcatctttt
     1981 tcttctccgt accatttggc gatcgctttt agtagatgct ctttcacggc gagtactgtt
     2041 cgtacgggac agtccagtgt gaatcgcagt cggtctgact gttcgggaag cagtggctca
     2101 ctcacggcaa ccagcaaatg atcactttta cgctcgagcg gcaacaaccc atattctcga
     2161 gcaacccggg cagggacaga atc
//
LOCUS       DAOPTR010000721         2180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39545_length_2180_cov_4.120941, whole genome shotgun sequence.
ACCESSION   DAOPTR010000721 DAOPTR010000000
VERSION     DAOPTR010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39545_length_2180_cov_4.120941"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="PKI05_12535"
     CDS             complement(<1..465)
                     /locus_tag="PKI05_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOA53071.1"
                     /translation="MRCRQCVFALGFVILVVAGAAGLEPGLRLNYPPDMPGAEVQTYK
                     KVDGVELKLYIYKPKDWKPSDRRGCIVFFFGGGWQSGSPAQFRPQCEYLASRGMVAIS
                     ADYRVASRHNAKVVDCVADAKSAIRWVRKNAEQLGVNPNKIAAGGGSAGGHLA"
     gene            complement(546..1625)
                     /locus_tag="PKI05_12540"
     CDS             complement(546..1625)
                     /locus_tag="PKI05_12540"
                     /inference="COORDINATES: protein motif:HMM:NF015617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-reducing end alpha-L-arabinofuranosidase
                     family hydrolase"
                     /protein_id="HOA53072.1"
                     /translation="MSAVDGVRVRSGVAVTLLVLAFAVFPVPIWAGEDIDGERHASPY
                     ETGSFRWTVGPPVLAARSSTPEMTYYSIKDPSVVRFDDRWHIFCTVRGRPRTHQVEYL
                     SLSDWQDPKPRREMLEIIPGYYCAPQVFYFRPHKKWYLLYQTSDPQRKPALQPAISTN
                     DRVDGVGKWSQPEFLFATGPNNVERWIDFWIICDDQRAYLFFTSLDGRFWRCETSLRD
                     FPHGWNEPKVVLQTDIFEAAHVYSLKGRSQYLAIIEAQNGSRRYYKAFLADRLDDDWR
                     PLAAERNKPFLGPQNVRFLGEEWCQSFSHGELLRDGYDERMEVNPDRLVMLFQGVSDR
                     EMAGKPYGEIPWKLGLAYQEVPAGP"
     gene            complement(1723..>2180)
                     /locus_tag="PKI05_12545"
     CDS             complement(1723..>2180)
                     /locus_tag="PKI05_12545"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOA53073.1"
                     /translation="DLGEFDDQSSRVTCVVDFFGPADMLTIGDYPSQMQHYAPDSPES
                     KLIGGPILENKEKARSASPITFVSKDDPPFLIVHGTDDPLVPYQQSVSLRDALKKVGV
                     PVALITVEKGGHGGFRNPEIERRVDSFFDAHLRDIKAEFKDETLPNESK"
BASE COUNT          497 a          619 c          588 g          476 t
ORIGIN      
        1 ggccaggtgt cccccagcag agccgccacc cgcagcgatt ttgttgggat tgacgccgag
       61 ttgttccgcg ttctttctta cccaacgaat ggccgatttg gcgtcggcca cgcaatcaac
      121 gaccttcgca ttgtgacggc tcgcaacgcg atagtccgcc gagatggcta ccatgccacg
      181 cgaggccaga tactcacatt ggggtcggaa ctgagccggc gacccgcttt gccagcctcc
      241 accaaagaag aagacgatgc acccacggcg gtcgctcggc ttccagtcct tgggtttgta
      301 gatgtaaagt ttcaactcca ccccatcaac tttcttgtat gtctggacct ccgccccggg
      361 catgtccggc gggtagttca ggcgaagacc aggttccaac cctgccgcac cagcaacaac
      421 taggatgaca aatcccaagg cgaaaacaca ttgacggcaa cgcatgactg aatcctccca
      481 ctgcattttt gacgaacacg cgaaattaac cactctttaa acgcgcaaga aaggcatacc
      541 tccggctatg gtccggcggg tacctcctga taagccaaac ccagtttcca tgggatttca
      601 ccgtaaggtt ttcctgccat ttctcggtca ctgacccctt gaaagagcat caccaaccga
      661 tccgggttga cctccatccg ttcgtcgtag ccatctcgca gtagctcgcc gtggctgaac
      721 gattggcacc actcctcgcc aaggaaccta acattctggg gtccgaggaa aggtttgttc
      781 cgttctgcgg ccagaggacg ccagtcgtca tccaatcggt cagccaggaa ggccttataa
      841 tagcggcggc ttccgttttg ggcctcgatg atagcgagat attgggatcg gcccttcagg
      901 gaatacacat gcgctgcttc gaagatgtcg gtctgaagaa cgaccttggg ttcattccat
      961 ccgtggggaa agtcacgtaa gctggtttcg catcgccaga agcggccatc aagcgaggtg
     1021 aaaaagaggt aagctcgttg gtcgtcgcat atgatccaaa aatcgatcca gcgttcgaca
     1081 ttgttcgggc ctgtggcgaa gagaaactca ggttgcgacc acttgcccac cccgtccaca
     1141 cggtcattgg tggaaatcgc cggttgaaga gcaggcttac gctgcgggtc gcttgtctga
     1201 tagagcaagt accatttttt gtgaggccga aaataaaaaa cctgcggcgc acaatagtaa
     1261 ccggggatga tttccaacat ctcgcgccgt ggtttggggt cttgccagtc gctgagactg
     1321 agatactcca cttgatgcgt tctgggacga ccacggaccg tgcagaagat gtgccatcga
     1381 tcgtcaaagc ggaccacact ggggtccttg atcgagtagt aggtcatttc cggtgtgctg
     1441 ctccgtgccg ccaggaccgg tggccccacc gtccagcgaa aggaacctgt ctcgtagggg
     1501 cttgcgtgcc tttcgccatc tatgtcctcg cctgcccaga tagggaccgg gaatacggca
     1561 aaggccaata ccagtagcgt caccgccaca cctgagcgga cacggacacc gtcaaccgcg
     1621 ctcatcagtt tgttcctccc tagacaatga acatgcataa tcttggcgag atgacttatc
     1681 gtgttgcacg atgagtcaca caaacgggtg ctgaactgat gatcacttac tttcattggg
     1741 aagcgtttca tccttgaatt cggccttgat gtcgcgtaag tgtgcgtcga agaaggaatc
     1801 gacccgccgc tcgatttctg ggttacgaaa accaccatgg ccacccttct cgacggtgat
     1861 caaggccaca gggacgccga ccttcttcag tgcatcgcgc agagataccg attgctggta
     1921 agggacaagc ggatcgtctg tgccgtgaac aataaggaac ggaggatcat ccttggacac
     1981 gaaggtgatg ggcgaagccg agcgggcctt ctccttgttc tccaaaatcg gcccaccgat
     2041 gagtttggat tctggtgaat ccggggcata gtgttgcatt tggctgggat aatcaccgat
     2101 ggtcaacatg tcggccgggc caaagaagtc gaccacgcag gttacccgac tgctttgatc
     2161 atcaaactca cccaggtctc
//
LOCUS       DAOPTR010000722         2172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39733_length_2172_cov_3.937648, whole genome shotgun sequence.
ACCESSION   DAOPTR010000722 DAOPTR010000000
VERSION     DAOPTR010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39733_length_2172_cov_3.937648"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            453..2006
                     /locus_tag="PKI05_12550"
     CDS             453..2006
                     /locus_tag="PKI05_12550"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOA53074.1"
                     /translation="MKTVDRNTPAVGIDLGTTYSVVAYLDAQGHPETLVNAEGERLTP
                     SVVFFDDEEVIVGREALKAFARLPERAAECSKRDVGYKVYHKVLQGKYYPPEVIEAYI
                     LNKLRQDAAQQIGDFSQVVITVPAYFDEVRRKATQDAGYMAGLDVMDIINEPVAAALA
                     FAYEAGFLSEASIPSRPERILVYDLGGGTFDVTVMEIRGHEYVTLAIDGDVRLGGWDW
                     DQRLVDLVAEAIIREHGFDPREDVRAWGKLWSLCEEAKRTLSVRPRATISFEYQGRTA
                     EIDVQREQFEAATQDLLDRTRFTTTQALKAAGLTWAEIDRVLLVGGATRMPMVRRLIR
                     DLVKKEPEVSVSPDEAVALGAAIHAGWLLGKAQGNPPRFRIRNVNSHSLGVVGIDPLT
                     RRPRVGTIIPRNTRLPIIAKRRFKTHRPNQRSILVRIVEGESPNPEDCCSIGQVVIRD
                     LPLNLPKGWPVEVIFHYRANGRLRVKIRVRHMEQDIVAEFRRERALTKELLDGWRRFI
                     SGKEPTDYE"
BASE COUNT          463 a          541 c          669 g          499 t
ORIGIN      
        1 ccccgggagg ccctgctgca tgagagaaag aaaaccggcc accaccgggg acagttggcg
       61 gatgagtcag ggcaaagcgt tgacatgggg tcacgccaaa agcgtttgcc ggccgtcggt
      121 tatcgcccgc atcgagctac cttccgccgc cgaccgctca attgacatga tttggggaca
      181 gtcgattgcc tgaaaatccg gacgatcctg cataatcggt ctgctttgta aagccaatgc
      241 tcaggaatct gcccggttgt gtaaccccgc ggttctctct ggggttcact cttgccccga
      301 gcgaactctg acaaggtaac attgatgtta gacggctctc ctatgagcgg agaatgattc
      361 gttgcgaacg gtagggccgt taaatatctg ggagctatag gggagtcaag aagccgagga
      421 tgatcgggca acttgcgtag ctggaaacgc caatgaaaac tgtcgatcga aatacaccgg
      481 ccgtcggaat tgatctgggg acgacttatt cggttgtggc ttatttagat gcccaagggc
      541 atccagaaac ccttgtcaat gccgaaggtg agaggcttac tccgagcgtt gtgttttttg
      601 atgatgaaga ggtcatagtt ggtcgcgagg ccttaaaggc gtttgcccgg cttcccgagc
      661 gtgcggccga gtgttccaaa cgagacgtcg gttacaaagt ctatcacaag gttctgcagg
      721 ggaaatacta tccgcccgaa gtgattgagg cttatatcct caataaactc cgccaggatg
      781 ccgcccagca gattggggac ttttcacaag tggtgatcac ggttccggcc tacttcgatg
      841 aagtgcggcg caaagcgacc caagatgctg gttacatggc cgggttggat gtgatggata
      901 tcatcaacga accggtggcg gctgcgttgg cattcgctta tgaagcgggt ttcctcagtg
      961 aggcttcgat tccctcgcgg ccggagcgga ttcttgtcta tgatctgggg ggagggacgt
     1021 ttgatgtgac cgtgatggag attcgaggac acgagtatgt gacattggcc attgacggtg
     1081 atgtccggtt agggggatgg gactgggatc aacgtcttgt cgatttggtg gctgaggcaa
     1141 tcattcgaga gcacggtttt gatccgcggg aggatgttcg cgcgtggggt aaactgtggt
     1201 cgctctgtga ggaagccaaa cggactctat cggtgcggcc gcgggcaacg atttccttcg
     1261 aataccaggg gcggacggcg gagattgacg ttcagcggga acaatttgaa gcggccacac
     1321 aggaccttct cgatcgcacc cgctttacga cgactcaagc cctgaaggcg gcggggttaa
     1381 cctgggccga gattgaccgc gtgcttcttg ttggtggtgc cactcgcatg cctatggtcc
     1441 gccggcttat tcgggaccta gtcaagaagg aacccgaagt gtctgtgtcc cctgatgagg
     1501 cggttgcctt aggggccgcg attcatgccg gctggctatt ggggaaagcc caaggaaacc
     1561 cgccgcgatt tcgcattcgc aacgttaact cgcacagtct tggcgtggtg ggaattgatc
     1621 ccctcacacg gcggccgcgg gtgggaacga tcattccccg aaatacgcgg ctgccaatca
     1681 ttgccaagcg gcgtttcaaa acacatcggc ccaaccagcg ttccattctc gtccgaattg
     1741 tcgaaggcga aagccccaat ccggaggact gctgttccat cggtcaagtg gtcatcaggg
     1801 atctgccttt gaaccttcct aaaggttggc cggtcgaggt gattttccat tatcgtgcta
     1861 acggccgcct gagagtgaaa attcgggtac ggcacatgga gcaggatatt gtcgccgagt
     1921 tccggagaga acgagccctg acgaaagagt tgttggatgg ctggcggcgg tttatcagtg
     1981 gtaaggagcc aaccgattac gaataagcgt tgttagcgca gagacggagt gtcacttttt
     2041 ttcaggcatc cgattggctt aaaccctatg ccataatctc ccctctcccc ttcagcggga
     2101 gaggggtcgg gggtgagggt ggctcgaatc gctgccggtt ttctcaatcg attctgacgt
     2161 tcgcttgccc tc
//
LOCUS       DAOPTR010000723         2172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39742_length_2172_cov_2.912140, whole genome shotgun sequence.
ACCESSION   DAOPTR010000723 DAOPTR010000000
VERSION     DAOPTR010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39742_length_2172_cov_2.912140"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PKI05_12555"
     CDS             <1..403
                     /locus_tag="PKI05_12555"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOA53075.1"
                     /translation="ADGFYEWKSEGRRKQPFFIRLRGDKLFAMAGLWDRWHAPTREVV
                     ETCTILTVQPNRVLAELHNRMPLILPPEAYSVWLDPSVTQFEALQDWLKPYPEEEMEL
                     VPVSTYVNAIHNEGARCIQPVAGFEASSGEG"
     gene            complement(361..1113)
                     /locus_tag="PKI05_12560"
     CDS             complement(361..1113)
                     /locus_tag="PKI05_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53076.1"
                     /translation="MLAQRVLKDVGLAAICVLLIIAVFYWRPFAPPDPTEQPAVGQPA
                     QFLEVYPLDKVLAAANAFPFQEPKPLTVEDLAGQVALVFFWNPRVDPSVDALRRLAAV
                     LPIVSRSDFRFLGVACPLQSDMETPGEFLSLTETTWKDCAIPLPCYLDPYGSSRTTLA
                     ILNQRGAEPAPRPAVVLPTAVLIDTDGLIRAVWEGWIPNQERDIARVVEKLLGPRATP
                     PEPPEGSTTSPHGGEAPTGHPSPEDASKPATG"
     gene            complement(1237..>2172)
                     /locus_tag="PKI05_12565"
     CDS             complement(1237..>2172)
                     /locus_tag="PKI05_12565"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOA53077.1"
                     /translation="NVGRMLEMARFAVLDYEPLFSVKLVVFPEFAQVAPVFGTVEELH
                     DRLAVPVPNEFTDRYWDLAKELGIYIQTSSFLERDDKYPGHVFNTTCLIGPPGILLKY
                     RKVHPWIPGEWQTSPHDIPGYDEPLFPVADTEIGKIGVAICYDWLFPEAIRQLALAGA
                     EVLIRVSAYMDPWGTASPLEWWTLINRVRAIENLAYVVACNQGASLRNYAPYSWPGGS
                     MIVDYDGRILAQAEPGAGERIVVGPIDLAALRYERQRRRGHFMLGHLRMEAYSGYQKS
                     IYPPGCFPRPPRISDIEQAIELGRQRWQQIAQKQR"
BASE COUNT          476 a          629 c          657 g          410 t
ORIGIN      
        1 ggccgacggt ttttacgagt ggaaaagcga aggccgaagg aaacagccct ttttcattcg
       61 cctgcggggt gacaagcttt tcgcgatggc cggtttatgg gatcggtggc acgcaccgac
      121 gcgcgaggtt gtcgagacgt gcacaatttt gaccgtgcag ccgaaccgtg tcttggcaga
      181 gctgcacaac cgaatgcccc tcattctgcc acccgaggcg tattccgtgt ggcttgatcc
      241 ctccgtgacg cagttcgaag cgttacagga ctggctaaaa ccctatcccg aagaggagat
      301 ggaactggtg ccggtgagta cctacgtcaa cgccatccac aatgagggtg cccgctgcat
      361 tcaaccggtg gccggttttg aggcgtcttc tggggaagga tgaccagttg gtgcctcgcc
      421 gccatggggt gacgtcgtgg agccctcggg tggttcggga ggcgtcgccc gtgggccgag
      481 gagcttttcc accactcgag cgatgtcgcg ctcctggttg ggaatccatc cctcccaaac
      541 ggcgcgaatc agcccatcgg tgtcgatgag gaccgctgtg ggcaatacga cggcgggtcg
      601 cggcgccggt tcggctcccc tctgatttag gattgccaac gtcgttcgac tcgacccgta
      661 agggtccaaa tagcaaggaa gtgggatggc acagtccttc caggtagttt cggtcaaaga
      721 gagaaattcg ccgggggttt ccatgtcact ttgcaaggga caggccacgc cgagaaagcg
      781 aaagtcgctc cgcgagacga taggaagaac cgcagccagg cggcgtaagg catcaacgct
      841 gggatcgacc cgggggttcc aaaaaaagac aagggcaacc tgccccgcaa ggtcttccac
      901 ggtcaggggt tttggctcct gaaaagggaa tgcgttggcc gcggcaagga ctttgtcaag
      961 cggataaacc tcgagaaact gagcaggctg gcccaccgca ggctgctcag ttgggtctgg
     1021 tggggcaaac ggccgccagt aaaaaacggc gatgatcaaa aggacgcaga tcgccgccag
     1081 ccccacatct ttaagaaccc gctgcgccaa cacgacttga gaggctctcc ttggcccgcc
     1141 gcggagaggc tctctgcgag ggaatgcaaa ggcacgaaga atacgacgcc tcaatgggtt
     1201 taacacggga cgtgcctcac aggggaaaca ctcatttcag cgttgctttt gagctatctg
     1261 ctgccagcgt tgcctgccca gctcgatggc ctgctcaatg tcgctgattc gcggcggtcg
     1321 gggaaagcat ccggggggat agatcgactt ctgataaccg ctgtaggcct ccatgcggag
     1381 atgccccagc atgaagtggc cgcgccgccg ctgccgctcg taacgcaaag ccgccagatc
     1441 aatggggccc acgacgatac gttccccggc cccgggttcg gcctgggcca ggattcggcc
     1501 atcgtaatcg acaatcatac ttccgcctgg ccagctatac ggcgcataat tccgcaagga
     1561 ggccccctga ttgcaggcca ccacataggc gaggttttca atggcccgca cccggttgat
     1621 caacgtccac cattccaaag gcgaggccgt tccccaaggg tccatgtagg cagagacacg
     1681 gataaggacc tccgcgcccg ccagggccaa ttggcggatg gcttcgggga aaagccagtc
     1741 gtaacagatt gccaccccaa ttttgccgat ctctgtatcc gccacgggga aaagcggctc
     1801 atcatatccg ggaatgtcgt gcgggctggt ttgccattca ccaggaatcc acgggtgcac
     1861 ttttcggtat ttcaggagga tccccggagg accaatcaaa catgtggtgt tgaagacatg
     1921 tccgggatac ttgtcatcgc gttccaaaaa actgctcgtc tgaatgtaga ttcccaactc
     1981 cttggccagg tcccaatatc ggtcggtgaa ctcgttaggg acaggcacgg ccaggcgatc
     2041 gtggagttct tccaccgtac caaacaccgg cgccacttgg gcgaactcgg gaaagaccac
     2101 cagcttcacc gagaacaggg gctcataatc gaggacggca aagcgggcca tctccagcat
     2161 ccggccaacg tt
//
LOCUS       DAOPTR010000724         2171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39759_length_2171_cov_3.851607, whole genome shotgun sequence.
ACCESSION   DAOPTR010000724 DAOPTR010000000
VERSION     DAOPTR010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39759_length_2171_cov_3.851607"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1567)
                     /locus_tag="PKI05_12570"
     CDS             complement(155..1567)
                     /locus_tag="PKI05_12570"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOA53078.1"
                     /translation="MTRPIFGVMLCLCLIATLSVSREINASEPSAVVIPKELPQERDG
                     RMKWWREARFGMFIHWGIYAIPGGRWNGKPVNTAGEWIMNGANIPVEEYEKLTKQFNP
                     VKYDPAEWVRIAKDAGMKYIVITSKHHDGFCLFDSKVTDYDVVDATPWGKDLLKPLAD
                     ECRKQGLHFCTYYSIMDWHHPAQMRGSDRAYNPTKIRDGRKREYLDYMKAQLRELLES
                     CRPEVLWFDGEWPDWYTEEDGREIYAYLRQLEPTLIINNRVGKGRKGMEGLNRDDQEY
                     VGDFGTPEQQIPPGGLPGIDWESCMTMNHTWGFKFDDHDWKSTETLIRNLIDCASKGG
                     NYLLNVGPTAEGEIPAASVERLQGVGRWMKVNGESIYATQASPWPNQLPWGRCTQKAI
                     DGKTTRLYLHVFQWPEDHRLELSGLTNTPVKAYLLATGAPLKVEKTSSGVVITDLPQQ
                     PVDAVATVICLDIEGQPKVE"
     gene            1798..>2171
                     /locus_tag="PKI05_12575"
     CDS             1798..>2171
                     /locus_tag="PKI05_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOA53079.1"
                     /translation="MDRRRGFTLVELLVVIAIIGLLIALLLPAVQAAREAARRMSCTN
                     NLKQIGLALHNFHDVHKSFPIGSPNKVCPGYETIPDWQYRWGPLAMLTPYMEQFNVYQ
                     SLNLNVPLYGHTGVFNGPGVGV"
BASE COUNT          460 a          617 c          566 g          528 t
ORIGIN      
        1 ggtgttctcg atcgactccg acggtcgctt gccctcaccc caaccctctc ccagatggag
       61 agggaacatt gcgataggct ctaaccttac ccgacctgga tgctctcgga tttcccgtca
      121 caatgatgtg gcaaggtctg ccactaccaa aacactattc caccttgggc tgaccttcga
      181 tgtccaggca tataaccgta gccacggcat cgaccggttg ttgcggcaga tcggtaatga
      241 ccacgccgct ggatgtcttt tccaccttta aaggtgctcc cgttgcgaga agatatgcct
      301 tgaccggcgt gttggtcagg ccagacaact ccaaacgatg gtcctccggc cactggaaga
      361 cgtggagata aagccgcgtt gtcttgccat cgatggcctt ttgggtgcag cggccccacg
      421 gaagctgatt gggccaaggg ctcgcttgag tggcatagat agattcgcca ttgaccttca
      481 tccatcggcc cacaccttgc aatcgttcca cggaagcggc cgggatctcg ccctccgcgg
      541 tcggtccgac attgagaagg taattccctc ccttggacgc gcagtcaata agattgcgga
      601 tcaaggtctc tgtgcttttc caatcgtgat catcgaactt gaagccccag gtgtgattca
      661 tcgtcatgca cgattcccaa tcgattcccg gcagtccccc cggcggaatc tgctgctccg
      721 gtgttccaaa atcgccgacg tattcttgat catcccggtt cagaccctcc atccccttgc
      781 ggcctttgcc cacacgattg ttgatgatca acgtgggttc aagctgacgc agataggcgt
      841 agatttcccg gccatcctct tcggtgtacc aatcgggcca ttcgccatcg aaccacaaca
      901 cttccgggcg gcagctctcg aggagttccc gcagttgggc cttcatgtaa tcgaggtatt
      961 cgcgttttcg gccatcgcgg attttcgttg ggttatacgc ccgatcgctt ccccgcattt
     1021 gcgccgggtg atgccaatcc atgatggagt aataggtgca gaaatgcagg ccttgcttgc
     1081 gacattcgtc ggctaagggc ttaaggaggt cctttcccca cggcgtggca tcgaccacgt
     1141 cgtaatcggt gactttggaa tcgaagaggc agaagccatc gtggtgcttg gaagtgatga
     1201 caatatattt catgccggcg tctttggcga ttctcaccca ttcagccgga tcgtacttga
     1261 ctgggttgaa ctgtttggtc aacttctcgt attcttcgac ggggatgtta gcgccgttca
     1321 tgatccattc gcccgccgtg ttgaccggct taccattcca tcgcccccca gggatagcat
     1381 agatgcccca gtggatgaac atgccgaagc gggcttcacg ccaccatttc atacggccat
     1441 cgcgttcctg aggcaattcc ttgggaataa caaccgccga cggctctgag gcattgatct
     1501 ctcgggagac tgatagtgtg gcgatcaagc acaggcacaa catcacgccg aagatgggtc
     1561 ttgtcataaa aagctctcct attgtggggt agagatgggc caggtcttca gattacatcc
     1621 ctcgacaacc tcgacggccg tcagaaggaa ctcacgaagc ttcagtttag gttcagcgcc
     1681 ttgaggaaag caagtagtgc gatcttctcg gtccttgaca ttgcttttta agtgggttag
     1741 tattacgatt tcttaaaacg taataccaac ccaagccgag gacgaaaggg aacaacaatg
     1801 gatcggcgac gcggttttac gctggtggaa ctcctagttg tgattgccat cattggattg
     1861 ttgattgcgc ttctcttgcc cgccgtgcaa gccgccagag aggcggcccg ccggatgagt
     1921 tgtacaaata accttaaaca aatcggcctg gcgctccata acttccatga tgttcataaa
     1981 tcgtttccca tcggctcgcc gaacaaggtt tgtcccggtt acgaaaccat ccccgattgg
     2041 cagtaccgct ggggcccgtt ggcgatgcta accccgtaca tggaacagtt caatgtctat
     2101 cagtcgctca acttgaatgt tcccctatac ggccacacgg gcgtcttcaa tggcccgggg
     2161 gtaggcgtcc a
//
LOCUS       DAOPTR010000725         2171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39772_length_2171_cov_2.462193, whole genome shotgun sequence.
ACCESSION   DAOPTR010000725 DAOPTR010000000
VERSION     DAOPTR010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39772_length_2171_cov_2.462193"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1461)
                     /locus_tag="PKI05_12580"
     CDS             complement(<1..1461)
                     /locus_tag="PKI05_12580"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOA53080.1"
                     /translation="MARGETNYRHSPSDWGTVVMKKGYLRLQKIGLAMRVALCAAMLC
                     TASLAESATGARPPLRVGVVQMAVAESLEANRDRIVIGINQATKHGVRVVVFPEGALR
                     SQENHNAAAVAEAIDVIRDTARHCNVYVVFGGYTYLPHLKKEANWMLVIGPDGRDVFR
                     YEKLYDNHHATMPGVFFINGIPCSAIICADRWLRGVEEIPVQQGAQISFELSNNFASE
                     WVAPFGWYWYVPRALRNNVWVVFANTGNKAPDAQDSPLPIRELKHGHSAIIAPDGQIV
                     AAAHDGAETIVVADIDHNQATRAEARARASSPVLAPFWQAGIRLQQGQVVEAPPLVPL
                     ESAVVDITLAAAQVTGDYSDLEPMIAEARLKGADMVIFPERATTENNLERLKLAARQH
                     RITVVVGMETQHQGARRNSAFVIGPDGSILTRYDQIAAVSPFRPGDNPSAMWFRVKGV
                     PAVVTIGRDALWTELAELAAVAGAQIHVHLDNDPATD"
     gene            1843..>2171
                     /locus_tag="PKI05_12585"
     CDS             1843..>2171
                     /locus_tag="PKI05_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53081.1"
                     /translation="MVGFCAPGVFYGALFSVSEHQAESQSAVMWEGKTMVEKRSVTHR
                     INRRAFVAGTSAALAMGMLESEAPSQTGGTSAVKLAIEGGEKAVTRSPGSGMRWGDRE
                     LKQLQEML"
BASE COUNT          474 a          603 c          605 g          489 t
ORIGIN      
        1 gtccgtagct ggatcattgt caaggtgtac atggatctgg gcacccgcca cggcggcgag
       61 ttcggccaac tcagtccaca gcgcatcgcg cccaatcgtg actaccgctg gcacaccctt
      121 cacacgaaac cacatggccg acggattgtc gcccggtcga aacggactga ctgccgctat
      181 ttgatcgtaa cgagtgagga tcgagccatc tggcccgatg acaaacgcag aattgcgacg
      241 tgctccctgg tgttgagttt ccatgccgac gactacggtg atccgatgct ggcgagcggc
      301 tagcttaagg cgttctaaat tattctccgt ggttgctcgc tcgggaaaga tcaccatatc
      361 ggcacctttc aaacgggctt cggcgatcat cggctctaaa tcagaatagt cgccggtcac
      421 ctgtgccgcg gccaacgtga tatccacgac ggcagattct agcggcacaa gcggtggggc
      481 ctcgaccacc tggccctgct gaagccttat ccctgcctgc caaaatggtg caagcacagg
      541 actactggcg cgggcccgcg cctctgcacg ggtggcttgg ttgtgatcta tgtcggccac
      601 gacaattgtt tccgcaccat cgtgggccgc tgcaacaatc tggccatcag gcgcaataat
      661 ggcactatgt ccgtgcttca actcgcggat agggagggga ctgtcctgcg catctggcgc
      721 tttattaccc gtgttggcaa agactaccca gacgttattt cgtaacgccc gcggaacata
      781 ccaataccac ccgaaagggg ccacccattc gctggcgaaa ttatttgaca attcaaagct
      841 aatctgggca ccttgttgga cgggaatttc ctccacacca cgaagccacc ggtcggcgca
      901 gatgatcgca ctacagggga tgccattgat gaaaaatacg cctggcattg tggcgtggtg
      961 attgtcgtag agtttttcgt accggaagac atcacgtcca tctggaccga ttacaagcat
     1021 ccagttggct tcctttttta ggtggggcaa atacgtgtat ccgccaaaca caacatagac
     1081 gttgcagtgc ctggcagtgt ctctaataac gtcgatggcc tccgccacag cggccgcatt
     1141 gtgattttct tggctccgca atgccccttc cggaaaaaca accacacgaa ctccgtgttt
     1201 tgtggcctgg ttaataccga tgacaatgcg atcacgattc gcttcaaggc tctcggccac
     1261 tgccatttgc acgacgccga ccctcaatgg cgggcgagca ccagttgcag actctgccag
     1321 tgaggctgta cacaacatgg cggcgcacaa tgcaacacgc attgccaaac caattttttg
     1381 cagcctcaga tatcctttct tcatgacgac ggttccccag tccgaggggc tatgccggta
     1441 attcgtttcg ccacgtgcca ttcgcttact ggttgcgaat gtatggcgtt tttttggtga
     1501 gaggacaacg cacgtgtcgc cgcttgtggg aaccggaaac ctcacccggg aaaggcatca
     1561 tttctatcgt ggtggcgggg ctaaaaccaa tggcccagcg cattctaacc gcgggtttga
     1621 tgagcgtcaa ttgaccacgc ggtagccagc cttcgtgtta tctcgcctaa accgtcagac
     1681 ccttcggttc gtggccatta taaaaatgcc caggtacgcg actgactcgc ttctcaaagt
     1741 tgttttggcg aacgacctca gcctattgac aaccggtatc ccgtgacgta acttgagcag
     1801 cggaaacatg aaatagcgat tgctggggac ttcggtcgcg gtgtggttgg cttttgcgcc
     1861 ccgggtgttt tttatggtgc cctttttagc gtatcggagc accaggcgga aagccaatcg
     1921 gcagtgatgt gggagggtaa gaccatggtg gaaaaacgca gtgtaaccca ccggattaac
     1981 cgccgtgcgt ttgtcgccgg aacttctgct gcgttggcga tggggatgct ggaatcggaa
     2041 gcaccaagcc agacgggagg cacctcggcg gtaaagttgg ctattgaagg cggcgagaag
     2101 gccgtgacgc gctcacctgg ttcggggatg cgttggggtg atcgcgaact caagcaactg
     2161 caagagatgc t
//
LOCUS       DAOPTR010000726         2170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39787_length_2170_cov_3.399527, whole genome shotgun sequence.
ACCESSION   DAOPTR010000726 DAOPTR010000000
VERSION     DAOPTR010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39787_length_2170_cov_3.399527"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1632)
                     /locus_tag="PKI05_12590"
     CDS             complement(<1..1632)
                     /locus_tag="PKI05_12590"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA53082.1"
                     /translation="MELAPKNLSDLLAAVRGPSEPSVSDSPLASQFEHIALLGWFTID
                     EATGTATPSRSLMMHITPGELETLTREILARLGQSNAIACSVGLAAGECWAFACRFVT
                     PFAAVVACVKRTSPEETLPTAEIDIFRWMTLLLATGIIRLENEYWELATRVEHHHAEQ
                     EMLRQAQIESLEETVLEREARLQQQQLRQLWEQKYRESQEANRAKTEFLSNISHELRT
                     PLTAILGYVDLASEAQDHHLIQDYLRRIRHNSDNLLKIVNDILDLAAAEAGRIKPRII
                     PFDPCQVAREAIELITPEVEQRNLKVHLEYFYPIPNLIHSDPVRIRQILLHLLSNAVK
                     FTEKGEVRVEVRWVQVDGGQSEIQYSVIDTGIGFSCDALPELLEPFRQGDNSPHRRFG
                     GNGIGLSIVRRFVQLLGGRLEVASSPGKGSRFTVAFEAPLPPGVSLRMANDDGSQQAV
                     PPSAAEQTAWGMMVPLPHARVLLVEDGLDNRRLITRILERAGVQVTAVENGLQGYQEA
                     VASAAEGRPYDVILMDMQMPVMDGYQATERLRAEGW"
     gene            complement(1680..>2170)
                     /locus_tag="PKI05_12595"
     CDS             complement(1680..>2170)
                     /locus_tag="PKI05_12595"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOA53083.1"
                     /translation="CARMLAMRLRFHNFEDMFLAGLLHDIGIILIDQHFHESFVRVIQ
                     SIKDGTTLVEIEKGILGFAHTELGEAVGVQWGFPDATRAAIRYHHQTASYRGEYLALV
                     RCVEVAHLICTLKGMSSIGTNLLRFNPESLSGLGMTANDVKVVADDLDREMAEQMSLL
                     QI"
BASE COUNT          470 a          637 c          592 g          471 t
ORIGIN      
        1 ccaaccctcg gcgcggaggc gttcggtggc ttggtagcca tccatcaccg gcatttgcat
       61 gtccatgagg atgacatcgt aaggccttcc ctcggccgcc gatgcaacgg cctcttggta
      121 cccttgtagt ccgttttcca cagccgtcac ctgcactccg gcacgttcca ggatgcgtgt
      181 gatgaggcgg cggttgtcca agccatcttc cacaagtaag acccgagcgt gcggaagggg
      241 caccatcatc ccccaggccg tttgctccgc ggccgaagga ggcaccgctt gttgcgaacc
      301 gtcatcgttt gccatacgca gtgaaacacc cggtggcagg ggagcctcaa aagctacggt
      361 aaaccggctg cctttgccgg ggctgctggc cacttccagt cggcctccca gcagttggac
      421 gaaacgtcgc acaatcgaaa gccctatccc gtttccccca aaacgacggt gcggggaatt
      481 gtctccctgg cggaaaggct cgagtaactc cggtagcgca tcgcaggaga atccaatgcc
      541 cgtgtcgata acgctgtatt gaatctcgct ctgtccgccg tccacctgga cccaccgcac
      601 ttcgacgcgg acttcgccct tttcggtgaa cttcaccgca ttgctcagga ggtgaagaag
      661 aatttggcga atccggactg gatcggaatg aatcaggttg gggatggggt aaaagtattc
      721 caggtgaacc tttaggttgc gctgttccac ttccggggtg atcaactcga tggcttcgcg
      781 cgcgacctgg cagggatcaa aggggataat gcgtggcttg atgcgaccgg cctccgccgc
      841 agcgaggtcc agaatatcgt tgacgatctt caatagattg tcactattgt gccgaatcct
      901 cctcagatag tcttgaatca gatgatgatc ttgggcttca ctcgccaggt caacgtatcc
      961 gaggatagcc gtcagtggcg tgcgtaattc gtggctgata ttggagagga actctgtctt
     1021 tgcccgattg gcttcctgcg attcacgata cttttgttcc cacagttgtc gaagttgctg
     1081 ctgctgcagg cgggcctcgc gttcaaggac agtctcttcc agtgactcga tttgggcctg
     1141 gcggagcatt tcctgctcgg cgtggtggtg ctccacccgc gtggccaact cccagtactc
     1201 attttccaac cgaatgattc ccgtggcgag gagaagcgtc atccaacgga aaatgtcaat
     1261 ctccgccgtg gggagcgtct cctcggggga cgtccgtttc acgcaggcga caacggctgc
     1321 gaatggagtg acaaaccgac aagcaaaggc ccaacattca cccgccgcaa ggccgacgga
     1381 gcaagctatc gcattggatt ggcccaagcg ggccagaatc tcccgagtca aggtctcgag
     1441 ttcgcccggc gtgatgtgca tcatcaggct gcgggagggc gtcgcggtgc ccgtggcttc
     1501 gtcaatggtg aaccatccaa gcaaggcaat gtgctcaaac tggctggcca agggactatc
     1561 tgacaccgaa ggttcagaag gtccccgcac ggcagccaga agatcactga ggtttttcgg
     1621 cgcaagttcc ataccatcat gcctttttca atcgcctgcc aaccttactg gggcaaccgt
     1681 cagatttgta agagtgacat ttgctcggcc atttcgcggt ccaggtcgtc agccacgacc
     1741 ttcacgtcat ttgccgtcat acccagccca cttaaagatt ccggattgaa acgcaacaaa
     1801 ttcgttccta tggatgacat ccccttcaag gtacaaataa gatgcgccac ctccacacag
     1861 cgcaccaatg ccaaatactc gccccgataa ctggccgtct gatggtgata gcgaatcgct
     1921 gcgcgggttg cgtcgggaaa gccccattgc acccccacgg cttcccctaa ctccgtatgg
     1981 gcaaatccca gaatgccctt ctcgatctcc accagtgtcg tgccgtcctt tatcgactgg
     2041 atgacgcgaa caaacgactc gtgaaaatgc tgatcaatga gaataatccc tatgtcgtgc
     2101 aataatccgg caaggaacat gtcctcaaag ttgtgaaagc gtagtcgcat ggccagcatg
     2161 cgagcacaga
//
LOCUS       DAOPTR010000727         2170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39792_length_2170_cov_2.476123, whole genome shotgun sequence.
ACCESSION   DAOPTR010000727 DAOPTR010000000
VERSION     DAOPTR010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39792_length_2170_cov_2.476123"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PKI05_12600"
     CDS             <1..561
                     /locus_tag="PKI05_12600"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53084.1"
                     /translation="KDELGIAGGLQDRVIQVYEGMVYMDFAREVMETIHGYPCGVYEP
                     IDLAYLPPLYLAYSRAAAEPTEVIHNDLRGRYERGEPAVREAMVQFAKLAEQGRKAIL
                     EKDWETLGRLMDSNFDLRRKILNLAPEHIEMVERARSLKVPAKFAGSGGAIVGLCPDE
                     TTFQKLREVMAEIDCEVLRPIVAPTS"
     gene            661..924
                     /locus_tag="PKI05_12605"
     CDS             661..924
                     /locus_tag="PKI05_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53085.1"
                     /translation="MTAVSCQPISQRAEAMAGALLRPAEAPLERQIPNREVSYYGHSV
                     YLDSCRIGCRNPDGGGSCNGLELAWGAGVLRSVVWNGQRTGLL"
     gene            complement(1297..>2170)
                     /locus_tag="PKI05_12610"
     CDS             complement(1297..>2170)
                     /locus_tag="PKI05_12610"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOA53086.1"
                     /translation="FVGPKKEGRSSPNLSFSGLLKMVLATQPATTPGAIIFQHGDPSW
                     FSKIKRPTSDENLPQATSLPRVSLRKPSRLARSFSWTVPHEAEAQPSAMADLLQWEPD
                     DSLRMGTVIHAWLSRVRWLDQDGLPNEDERRTVALELLGSLDQFARLDSFFQEMLQRP
                     TIRSLLSLTSYQDMVTGQEHTPAHNRPGLQNPKWELYLEQPFVIRGDGAYTRGQFDRV
                     VVLLDGQHVVGADIIDFKSDNVTSDRLKERIEFYRPQLELYHRAAAEWLHVPESEVSA
                     RIAFVHSGIVVRVY"
BASE COUNT          474 a          552 c          658 g          486 t
ORIGIN      
        1 aaggacgagc tcggcatcgc cggcggtttg caggatcggg tgatccaagt gtatgagggc
       61 atggtttaca tggattttgc ccgcgaagtg atggaaacga ttcacggcta tccgtgcgga
      121 gtctatgaac cgattgacct agcctattta ccaccgcttt atttggccta cagccgggcg
      181 gctgcggaac cgaccgaggt catccacaac gatttgcggg gacgctacga gcggggtgaa
      241 ccggccgtca gggaggcgat ggtacagttt gcgaagttgg cagagcaggg gcgcaaagcc
      301 attttggaga aagactggga aacactggga cgcttgatgg acagtaattt cgatctccgc
      361 cgcaagattc tcaatctggc gccggagcat atcgagatgg tcgaacgggc gagaagcctt
      421 aaggttcccg caaaatttgc cggttcgggt ggtgccatcg ttggattgtg tcccgacgag
      481 accacattcc aaaagctccg cgaggtcatg gccgagatcg attgcgaggt cttgcgtccg
      541 atcgtcgcgc caacatcttg acgacctgcc aaaatttgac gtctctgcgc gagactcgcc
      601 ttctgcgaag tttattgatg ggatggattt tgcggaattt agggggacta ggaaaacagg
      661 atgactgctg ttagttgcca gccgatatcc caaagggcag aggcgatggc cggtgcgctg
      721 ctgcgaccgg ccgaggctcc tcttgagaga cagattccca acagggaggt gagttactat
      781 ggtcactcgg tttatttgga ttcttgccgg attggttgtc gtaatcccga tggtggcggc
      841 tcttgcaacg gactggaact cgcttggggc gccggcgtgt tgcggtccgt cgtttggaac
      901 ggtcagcgta cagggttgct gtgagctccc gccttcctgc tgtcggcacg tctgggatgg
      961 ttattgtgag acgaaaatgg cgaggggtaa tctttgcccc tgggggctaa gagggccgtg
     1021 cctcatcggc cggcgggtat gtctgccatg caccgggggt gtgcctcaag aatgcggagc
     1081 accattgcct gttccggatg aagggtcaag tatgtcgcaa ttgccagcgc aggaaactga
     1141 agcggcatca tcgccggcga tcatcaggtt caggactgtc actaaggcag agacccaaca
     1201 accgcctcgt cctttagagc cggtcattcc acttccgccg attgaaccgg aaaagaaaga
     1261 gtgatcctct gtcaggccga cgttgctttc tagggattag taaacccgga ctacgatccc
     1321 tgaatgaaca aaggcaattc gagctgaaac ttcggattca ggcacgtgga gccactcggc
     1381 tgccgctcgg tgatacaact caagctgtgg gcggtagaat tcgatccgct ctttgagcct
     1441 gtctgatgtc acattatccg atttgaaatc aataatatcg gcccccacca catgttggcc
     1501 atcaagcaga accacaaccc gatcaaattg cccgcgggtg taggcaccat cgccacgaat
     1561 cacaaaaggt tgctcaagat ataactccca ttttggattt tgaagacctg ggcgattatg
     1621 ggccggtgtg tgctcctgac cggtgaccat gtcctgataa ctggttaggg agagaagact
     1681 gcggatcgtt ggccgttgca acatctcttg gaaaaagctg tccaacctcg caaattggtc
     1741 taacgatcca aggagttcca gggctacggt gcgtcgttca tcctcgttag gcaggccatc
     1801 ctgatcaagc cagcgcaccc gcgacagcca ggcgtggatc accgttccca tgcggaggct
     1861 atcgtcgggc tcccattgca ataggtcggc cattgccgag ggctgggcct ccgcctcatg
     1921 gggaactgtc caagagaacg atctcgccaa ccttgaaggt tttcgcaggg aaactcgggg
     1981 cagactggtc gcttggggga gattctcgtc acttgtgggt ctcttgattt tcgagaacca
     2041 agacgggtca ccgtgttgaa agatgatggc gccgggagtg gtcgccggtt gcgttgccaa
     2101 taccatcttg agcaaaccgg agaaactcaa gttgggggag gagcgccctt cttttttggg
     2161 gccaacaaac
//
LOCUS       DAOPTR010000728         2169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39812_length_2169_cov_2.612110, whole genome shotgun sequence.
ACCESSION   DAOPTR010000728 DAOPTR010000000
VERSION     DAOPTR010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39812_length_2169_cov_2.612110"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="PKI05_12615"
     CDS             <1..980
                     /locus_tag="PKI05_12615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA53087.1"
                     /translation="WHQLIPVIVRFARMLHQEKVQILQSFLFHANIVSRIAARFAGVP
                     VVISGLRVAEHAARWHVLVDCMTSGCVDHYVCVSQSVAQFAVRNGLPPEKIRVIPNAV
                     DPPEFCQERPQGTYGADKFLPEHIGRNEQNTTAPSCGRTAAGSEYRMVTVARLEYQKG
                     VDWLILALARWLRSYPDLRLVIVGDGPMKSTLQALIHERGLSQQVTLVGFQRDVARIL
                     CDADLFVLPSRWEGMPNALLEAMVCGLPVVATDVEGVREILGPCIAHQLVPFGDSDTL
                     THAILWHYLNRDCSQTIGQQNRTYVLSSFSWPRVVEQYEKLWLWLLQRG"
BASE COUNT          483 a          519 c          629 g          538 t
ORIGIN      
        1 actggcatca gttgattccg gtcatcgttc ggttcgcccg catgttacat caagaaaagg
       61 ttcaaattct ccagtcgttc ctttttcatg caaatatcgt ttcgcgcatt gctgctcgct
      121 ttgccggcgt tccggtcgtt atttcgggct tgagagtcgc tgagcatgca gcacgttggc
      181 atgtgcttgt ggattgtatg accagcgggt gtgtcgacca ctacgtttgt gtcagtcaaa
      241 gcgtggccca atttgcggtc cgcaatggtc tgccacctga gaagattcgg gttattccaa
      301 atgccgttga tccacctgaa ttctgtcaag aaagaccaca aggaacatac ggcgcggata
      361 agtttttgcc cgaacatatt ggacgcaatg agcaaaatac cacggcaccg tcgtgtggtc
      421 gaacggctgc tggatcggaa taccgcatgg tgacggtagc gcggctcgag taccaaaagg
      481 gagtcgactg gttaatcctg gctctcgctc gttggcttag atcgtatcct gatctgcgac
      541 tggtcatcgt gggagacggc ccgatgaagt caacactgca ggcgttgatt cacgagcggg
      601 ggctctcaca gcaggtgacg ctggtcggtt ttcaaagaga tgtggcaaga atcctttgtg
      661 atgccgatct ttttgttttg ccttctcgtt gggaaggcat gcccaatgcc cttcttgaag
      721 ctatggtctg tgggctgccc gtcgtcgcca cagatgtcga aggtgttcgc gaaatcttgg
      781 ggccttgcat agcacaccaa cttgttccat tcggagattc cgatacgctc acgcatgcaa
      841 tcttgtggca ttatttgaac cgggattgct ctcagacgat tggacaacaa aatcgcacgt
      901 atgtgttgtc gtcgttttct tggccgcgtg ttgttgagca gtatgaaaag ctttggttgt
      961 ggcttcttca gcggggataa ttgaagcgaa gcaatgcgtg agcgctgggg taaatggact
     1021 ggggggaccg acgaaatggg acacccactt ttggagaatg ccggaccaac cgcgcacaaa
     1081 aagtacattt tttgccgtat cgggtgaatg gctaccgttt actactttga ccggcccatg
     1141 cgggggacgt gggcgccttg ttcctcggtt cggcgaggaa aaggattgtg ttgaacacga
     1201 gaactaatcg ggctttccgc tgctacggga aattctgggg gcggatgata tggtagctac
     1261 gggcttcgta gggggaacaa atggatgcac tcgatgggag gccatcctta atcgtgcggg
     1321 ggacatccct ccccgactgg tggctccagg agaccccgct caaaagcggc ccccggtata
     1381 ttgcggtacc tgccgttcgc tcgtcaaaag gtggtaccgt aacgattgac ggttatgaca
     1441 gaatatccgt tcgaggggct atgtaagcgg aactctcaat ttactgaaat tcatgttacg
     1501 ttgaagaaac ttgccttgtc agtcaagaga aactataaaa atagtcgacg gcggacggcg
     1561 ttcgttagcc agttttgtca acctatcggg ccacggttcg gatcgaaaaa atcgggcgga
     1621 taacatcaat atgccatttg actatgggcc gtgatctgcg catcatgaaa aacgctgggt
     1681 tttggaatgc ggagacagga gctaactgac agcggtcaat gcttagatca ggtgaagacg
     1741 tccgtccgag ctgtcgcatg aggtcggggt agcaaacgtt ggcgacttgg ccaccggcca
     1801 acgttcaccc ggttctgcca cgtgccggtt taccccgccc cgcctgatgt cagggacgtc
     1861 atcaggagcg acatgcgagc cccagccacg ccgtggagat acggggctca agggcctatc
     1921 ctccgaggat cgtaggaatg cgaaggagga taggcctttt cttttctgtt cgggtaagaa
     1981 ctcttctgga acatgggctt tccattgatc gcgagctgct cgctaaagtg accaaaaccc
     2041 tatgcgtgca ttgtcgagaa gcaagaacta taatgggcga cagttgcgtg atttgcaggc
     2101 gacgtccagt gtcgtggatt tgactcagca tccgttgggg aggtgtacca atgtctcttc
     2161 aaacaaact
//
LOCUS       DAOPTR010000729         2168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39824_length_2168_cov_3.832466, whole genome shotgun sequence.
ACCESSION   DAOPTR010000729 DAOPTR010000000
VERSION     DAOPTR010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39824_length_2168_cov_3.832466"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..915
                     /locus_tag="PKI05_12620"
     CDS             139..915
                     /locus_tag="PKI05_12620"
                     /inference="COORDINATES: protein motif:HMM:NF025446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4261 domain-containing protein"
                     /protein_id="HOA53088.1"
                     /translation="MKSDVFFAIVLLKDPKSVSVPKMAEFVRTHWADAPSLTELDADP
                     VVSPEGSLLMPFMVNGEPAFLVVEDKPVPEEDLELAVLTAWYWPEASQAAEEHRAFAM
                     VVLPSAPADPLEKTAIVTQLTAALAMTSQAVAVFWAKSNLIHEPEAFFNSAKHLGSGV
                     YPIELWVGFHAEPEPDHTITFFTRGMLNFGLPEIEVYNSTRDLQFIYERVFNVAHFLF
                     EHGPVIKDQETVGTNEDEQFVVTIGPSRFDARIKTMQIDM"
     gene            1088..>2168
                     /locus_tag="PKI05_12625"
     CDS             1088..>2168
                     /locus_tag="PKI05_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOA53089.1"
                     /translation="MLYAVILAGGSGTRLWPESRSALPKQLLPIHGSQSLLRATVVRA
                     SQFVPYERIIIATSRDLAPAVQEELSFLPREALLVEPAPRNTAPCIGLAALRLLHNDP
                     EAVMVVMPADHVIEPQEAFASDIDLAVQVIEEDPTRLVTFGIVPHYPSPSFGYIERGE
                     ALSVAPASPTMPGSSGSMEDRPRVFRAKSFREKPSIQVAEGYLRAGNFYWNAGIFVWK
                     AKTIWDLLQQHQPSVAEPLAHILSASQSPNFPQILESEFSKAEKISIDYAVMEKADNV
                     VVVEAQFRWDDVGSWRSLERLLPADNCGNVSDAERCLLLDTTGCIVRCRDPRHLVATL
                     GVDNLVIVITPDATLVARKDREEDIR"
BASE COUNT          458 a          631 c          579 g          500 t
ORIGIN      
        1 tgccctaaaa tgcgggagtc ttttggcggc ctgtggccca tggggctccg gaggtaggaa
       61 cgttgcgaaa actcgtcccg ctggacttca ggcaccaaca agtaacgatt tttgttgtat
      121 aagtgtgaga ggtgctttat gaagtcggac gtgttcttcg ccatcgtcct gctcaaagac
      181 cccaaaagtg tgtctgtacc aaagatggcc gaatttgtgc ggacacattg ggcagatgcc
      241 ccgtctctca ccgagttaga tgccgatcct gtcgtaagcc ccgagggcag cttgctcatg
      301 cccttcatgg tgaacggcga acctgccttc ctggtcgtgg aagataaacc agtgcctgag
      361 gaggatttgg aactcgccgt tctcacagcc tggtactggc cagaagcgtc acaagccgcg
      421 gaagaacatc gggcgtttgc catggtggtc cttccctccg cgcctgccga tcctttggaa
      481 aaaacggcga ttgtcacaca attgacagca gcactggcca tgacctcgca ggctgtggcc
      541 gttttctggg cgaaatccaa tttgatccac gaaccggagg ctttcttcaa ctcggccaag
      601 cacctcggct cgggcgtcta tcccatcgag ctatgggtcg gcttccacgc cgaaccggaa
      661 ccggatcata ccatcacctt ctttacgcga ggcatgttga attttggact gcccgaaatc
      721 gaggtctata acagtacccg cgatttacag tttatctatg agcgggtatt caatgtcgcc
      781 cacttcctat ttgagcacgg acctgtcatc aaggatcaag agacggtcgg gacaaacgag
      841 gatgagcaat ttgtggtgac catcgggcca tctcgctttg atgcccgaat caaaaccatg
      901 caaatcgaca tgtaaagcaa tcggcagtcc ggcccttttc agccttccac ttttggggca
      961 acacaccctt gggaagcgat accgactcct tttatcatgg tgatatccta tttgggactt
     1021 gccgtcggaa atcgctagca ccctcccagg gtgcctcaat attccgtggt aacagggaga
     1081 caccacaatg ctttatgctg tcattttggc gggtggctca ggaacccgac tttggccgga
     1141 aagtcgtagc gccctcccta aacagctttt gccgatccac gggtcgcaga gtctcctgcg
     1201 ggcgactgtg gtccgcgcgt cacagtttgt cccctacgaa cggatcatca tcgcgactag
     1261 ccgggatttg gccccggcgg tacaagaaga gttgtccttt cttccccgcg aggccctcct
     1321 tgtcgaacca gccccccgaa atacggcccc ctgtatcgga ttggcagcgc tgcggcttct
     1381 ccataacgac ccggaggccg tgatggtcgt catgcccgcg gaccacgtga tcgaaccaca
     1441 agaggccttt gcctccgata tcgatttagc agttcaagtg attgaggaag atcctacccg
     1501 gctggtgacg ttcgggatcg ttccccatta tccttccccc tcctttgggt acattgaacg
     1561 cggcgaggcc ttgtccgttg ctccagcgtc ccccacgatg cccggcagtt cgggcagcat
     1621 ggaagaccga cctcgcgtct ttcgcgccaa aagcttccgc gagaagccct ccattcaggt
     1681 agcagaagga taccttcgcg cgggcaattt ttactggaac gccggaatct ttgtctggaa
     1741 agcgaaaacg atctgggacc ttttgcagca acaccagccc agtgtggccg agccgcttgc
     1801 ccacattctc tcggcttctc agtcgcccaa ttttccccag attcttgaat ccgagttttc
     1861 caaggcagaa aaaatctcca tcgattatgc tgtcatggaa aaggccgaca acgttgtcgt
     1921 ggtcgaagcc caattccgat gggatgatgt cgggagttgg cgatctttgg agcggcttct
     1981 tccggcggac aactgtggca atgtctcgga cgcggaacga tgtctccttt tggacaccac
     2041 ggggtgcatc gtgaggtgcc gcgatccccg tcaccttgtg gccaccctgg gcgtggataa
     2101 cctggtgatt gtcatcacac cagatgccac cctcgttgcc cgaaaggacc gcgaagaaga
     2161 catccgca
//
LOCUS       DAOPTR010000730         2168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39830_length_2168_cov_3.150970, whole genome shotgun sequence.
ACCESSION   DAOPTR010000730 DAOPTR010000000
VERSION     DAOPTR010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39830_length_2168_cov_3.150970"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..>2168)
                     /locus_tag="PKI05_12630"
     CDS             complement(43..>2168)
                     /locus_tag="PKI05_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53090.1"
                     /translation="WIGGRAPCSESAIAVLGDQECVYAVQMATGQVLWQRFVGGIFAE
                     YPSNTYPRIAVGGQETVAIPDPSSQGVILVDAATGHISRFIRLGEPPASWVLTERSYL
                     LVLGRSGNLLILNPLESQKVGTFRFPQPAAYPPAVDPVTRRAFILGEHSHLFVIRLDS
                     RECEQVVYLGHERGQIVAPPALVSGFLVIPERRGSTEGRLRVVNIQGGATDGLRVVQT
                     IDLPRPVAGPLQVLGTRFVVITSDGQLRVYQLRGGDEKQPFGLLAEGKTPDPTASQKG
                     WAVPRFVLFHKDQVLAADMALTLFELQASAGKLVPRWVACQETLALSSPVVQDSTIFY
                     IYRYPDRPGVFITAVDVESGRVFWESQVADPPLMEAHLTEDGQKAQILGQSGNLYLLE
                     PNAASSMLAGIRPDRVLRFPGLSEGKAPIGCVLPSKTRVIGILGNRELTVLEPERDRM
                     RVASLPLPEGLGSAPVPFRNGVLVALEDGTLANLDVTTGRPLGEPYQCEMAAGARVTW
                     SDLVALDETTLLAANSAGWLLKLRWAEKPNGHFQAEHSRLFDAAIVSPLAILDDTIIA
                     VDSKMRSLAISLSDLNTQRTQPLDSDLVWGPWSLSTAVCFATADGTLVLSTPQLEFTK
                     VNLEGELPVGKPVVTNNHLVFATRQGSVFCVDFARGTVVNRINLRMTCCTGPTIVGEN
                     VLLGSLDGRWIMIPKTDVLHAATKD"
BASE COUNT          506 a          610 c          624 g          428 t
ORIGIN      
        1 tgcctttcgc aatggagttg gtgaaaggtt gtcagaagaa gcttagtcct tggtggcagc
       61 atgtaacacg tcagtttttg gaatcatgat ccagcgtccg tccagactcc ccaacaagac
      121 attttctccg acgatagtcg gaccagtgca gcaagtcata cggaggttga ttcggttcac
      181 caccgtgccg cgagcgaaat caacacaaaa gacagatccc tgtcgtgtgg cgaaaacgag
      241 atggttgttg gtcacaaccg gcttacccac ggggagctca ccttctaaat tgaccttggt
      301 gaattcgagc tggggtgtgg aaaggaccag cgtgccatcg gccgtggcaa agcatacggc
      361 ggtggagaga ctccacggcc cccacacgag gtcgctatcc agcggttgtg tccgctgcgt
      421 attaaggtcg ctcagtgaga tcgccaagct cctcatcttc gagtcaacgg caatgatggt
      481 atcatccaag atcgccaacg gtgacacgat tgccgcatca aaaaggcgac tgtgttctgc
      541 ctggaaatgt ccgttgggtt tctcggccca acgtagcttg aggagccacc cggccgagtt
      601 ggctgctaag agggtcgtct cgtccagggc aacaagatca ctccacgtga cccttgctcc
      661 ggctgccatt tcgcattggt atggttcacc tagcgggcgc ccggtggtca cgtcgaggtt
      721 ggccagggtt ccgtcctcca aagcgacgag aacgccattt cgaaacggaa cgggagcgga
      781 accgagccct tctggcaaag gaagtgacgc aacacgcatg cgatcgcgtt ctggttcaag
      841 aacagtaagt tctcgatttc ccaggatgcc gattacccgc gttttcgatg gcaggacaca
      901 accaatgggc gccttgcctt ccgaaagtcc cggaaaccgc agaacgcggt caggtcgaat
      961 tccggccaac atcgatgaag ccgcattagg ctcaaggaga tacaggtttc cactctgccc
     1021 aagaatctgg gccttttgac cgtcttcggt aaggtgtgcc tccatcagcg gagggtcggc
     1081 cacctggctt tcccaaaaaa cacggccact ttccacgtca accgctgtta tgaaaacccc
     1141 tggccgatcg ggataacgat agatgtagaa gatagttgaa tcttgaacga cgggggacga
     1201 gagggcaagc gtttcctgac aggcgaccca ccgaggaacg agctttccgg cggatgcctg
     1261 tagttcgaag agcgtcaagg ccatgtcggc cgcaagcacc tggtccttat gaaagagtac
     1321 gaaacgaggg accgcccacc ccttctggga agctgtagga tccggcgttt ttccttccgc
     1381 aagcaaaccg aacggttgct tttcgtcccc gccgcggagt tgataaaccc gtaactgccc
     1441 atcgcttgta atgaccacaa atcgcgtgcc gaggacctgg agcggaccgg ccaccgggcg
     1501 gggcaagtcg atcgtttgga cgacccgcag accgtccgtc gcaccacctt gaatattaac
     1561 cacccgaagt cgtccttcgg tagaacctcg ccgttcgggt atcacgagga aaccggaaac
     1621 aagggcaggc ggtgcgacga tttgtccacg ttcatggcca agataaacga cttgctcaca
     1681 ctctcgcgaa tcaagccgaa tgacgaacag atgggaatgt tcgcccagga taaacgcccg
     1741 gcgggtcacg ggatcaacag cgggaggata ggccgccggt tgtggaaagc gaaatgttcc
     1801 cactttttgg ctttcaagtg ggttgaggat aaggagattt cctgaccggc cgagcacgag
     1861 taggtaactt cgctcagtca gcacccaact ggcgggtggc tcgcccaagc gaatgaagcg
     1921 actgatgtgc cccgttgccg cgtcaaccag gatcacgccc tgggaggatg ggtcgggtat
     1981 agcgactgtt tcctggccgc ccacggcaat ccgtggatac gtgttcgaag gatactctgc
     2041 gaagatacca ccgacaaacc gctgccagag aacttggcca gtggccatct gcacggcata
     2101 aacgcattcc tgatcaccga ggacagcgat ggcactctct gaacaggggg cccgaccgcc
     2161 gatccagg
//
LOCUS       DAOPTR010000731         2164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39926_length_2164_cov_3.893789, whole genome shotgun sequence.
ACCESSION   DAOPTR010000731 DAOPTR010000000
VERSION     DAOPTR010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39926_length_2164_cov_3.893789"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PKI05_12635"
     CDS             complement(<1..352)
                     /locus_tag="PKI05_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53091.1"
                     /translation="MAQITDQSKNAPPSLTRRQFVTAALAGGLSLYSREIAKLDSGEE
                     TSAGSDIIIDAAFPGGNILVERIDGDTVYLAQDLRDTEGWWFYWYFRVRGGAGRTVTF
                     RFTNKDVFTCQGPAM"
     gene            complement(453..>2164)
                     /locus_tag="PKI05_12640"
     CDS             complement(453..>2164)
                     /locus_tag="PKI05_12640"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOA53092.1"
                     /translation="PRQGSPFRLRDIPIMLTTLSRTWYLPCLLGLLPLAWPGHFSTAV
                     GDSSKSHDAAFLAALHSITADELRNHVGFLASDELEGREAGMPGGQTAGEYLAAALAQ
                     YGLQPNGSNGFFQDFGQGYRNVVAVLPGSAPELANEVIVLGAHYDHVGYGGKRNVRDY
                     AGLVHNGADDNASGTAAILEIAQAATLLQRPPRRTLLFILFDAEEKGLLGSKHWTENP
                     TRPLEQVKFMFNLDMVGRLRDQRVEVYGWRSSAGLRRLICQTNQGMNFDLDFSWRNRF
                     DSDHAPFFRKSIPVLLLHTGLHDDYHRATDDFDKINYDGMEQIARWVFEMAVALADGD
                     TAPGFRDKVNEDSDGKLREILEAPANWRDRIGIKVVSDQSTEAARQDGALRVEKVFFQ
                     TPAEAAGIQLGDRIIAVNGRRVSNPDEFAAATLLAKESSVRLRLRRNFGQGPEVEIDV
                     PLIGLPIRLGIQWYVDEAVPQSVMVSHILPGSPADSAGIAVGDYIYSVNGQAIEDPDQ
                     FDRYIREAASPLRLEVEHRGRIRTVEIPPSGSGQQVFWNHPMPLVKLAGHSITSQEFV
                     CLQ"
BASE COUNT          487 a          609 c          615 g          453 t
ORIGIN      
        1 tcatggcggg accttggcat gtgaaaacat ccttattagt gaaacgaaac gtcacggtcc
       61 ggccggcgcc acctcggaca cgaaaatacc aataaaacca ccaaccttcg gtgtcgcgga
      121 ggtcctgggc aagatagacg gtatctccgt cgatgcgttc tactaggatg tttccgccgg
      181 gaaaggccgc atcaatgatt atgtcgctgc ccgccgaagt ttcttcgcca gagtcaagct
      241 tcgcgatttc ccgggagtaa agcgataggc caccagccaa cgccgcggtg acaaactggc
      301 gtctcgtcaa agacggcggt gcattcttgg attgatcggt aatctgcgcc atgctatgcc
      361 tcccactgca accgtgatga cgtaacggct gttattgcga acccttgcct gctccgtcaa
      421 tgtggcattc ccgatgtagc acgggggagt ccctattgta agcagacaaa ctcctgcgat
      481 gttattgagt ggcccgcgag tttgaccaaa ggcatcggat ggttccaaaa aacctgttgg
      541 ccgctaccgg aaggcgggat ttccacggtg cgaatccgac cgcgatgttc gacttccaat
      601 cgcaaaggtg atgcggcctc ccgaatgtaa cgatcaaact gatcggggtc ttcgatggcc
      661 tgaccattca cggagtagat gtaatcccca acggcaattc cggcactgtc ggcgggcgaa
      721 ccaggaagga tgtgactgac catgacgctc tgcggcaccg cctcgtccac ataccattga
      781 atccccagac ggatgggaag accgattagc ggcacatcga tttcgacctc ggggccctgg
      841 ccgaagttgc gtcgcaacct caaccgaaca ctggattcct ttgccagaag cgtggcggcg
      901 gcgaattcgt ccgggttaga aactcgacga ccgttgaccg caatgatgcg atccccaagt
      961 tgaatccctg cggcttctgc gggcgtttga aaaaagacct tttccacacg aagggcacca
     1021 tcttgtcggg cggcttcggt ggattggtcg gaaaccacct tgatcccaat acgatcccgc
     1081 cagttggccg gggcctcgag gatttcccta agcttgccgt cagagtcctc attgaccttg
     1141 tcgcgaaaac cgggcgctgt gtcgccatcg gccaaggcaa cggccatttc gaagacccag
     1201 cgggcgattt gttccattcc gtcatagttg atcttatcaa aatcgtcggt ggccctgtgg
     1261 taatcgtcat gaagacctgt gtgaagtaat aggacgggga ttgatttacg aaagaatggg
     1321 gcgtgatccg agtcaaaacg gttacgccag gagaagtcca gatcgaagtt cattccctga
     1381 ttggtctgac aaatgaggcg acggagacct gcgctggaac gccatccata gacctccacc
     1441 cgttgatcac ggaggcgtcc gaccatgtcg agattgaaca tgaacttgac ctgctccagt
     1501 ggacgggtag ggttttcggt ccagtgcttt gatcctaaca ggcctttttc ctcagcgtcg
     1561 aagagaatga acaaaagtgt ccgccgcggt ggacgctgaa ggagtgtggc agcctgagca
     1621 atttccaaga ttgccgctgt cccactggca ttatcgtccg ccccattgtg gactaaaccg
     1681 gcgtagtcgc ggacgtttcg tttgcccccg tacccgacat gatcgtagtg tgcacccagg
     1741 acgatgactt cattggccag ttccggagca ctgccgggca ggacggccac cacattgcgg
     1801 taaccttgtc cgaagtcttg aaaaaagccg ttcgacccat tcggctgaag gccgtactgc
     1861 gccagggccg ccgccagata ctcgcccgca gtttggcccc ccggcatgcc ggcctcgcgc
     1921 ccttccagtt cgtccgaggc tagaaagccc acgtgattgc ggagctcgtc ggccgttatg
     1981 gaatgcagcg ccgcgaggaa ggccgcatcg tgggacttcg agctgtcgcc aacggccgtg
     2041 ctaaaatgac ctggccacgc aagcgggagc aaaccgagaa gacagggcaa ataccaggta
     2101 cgtgataacg ttgttagcat gattgggatg tctcgcaaac ggaaagggga accttgccta
     2161 ggat
//
LOCUS       DAOPTR010000732         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_39993_length_2161_cov_4.278727, whole genome shotgun sequence.
ACCESSION   DAOPTR010000732 DAOPTR010000000
VERSION     DAOPTR010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39993_length_2161_cov_4.278727"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2161)
                     /locus_tag="PKI05_12645"
     CDS             complement(<1..>2161)
                     /locus_tag="PKI05_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53093.1"
                     /translation="EALKQAPAEGRVLMYFGDGLSRANVLSAETLSQLLTGLVDNKIP
                     VSAYLIGTQVDFGLVASLATHTGGQLVVDSGDLDAAAVGERLSDAVRATVAFPNGAAW
                     SEQVAEVYPSRMPPLRSDRETVAVGTLKNPGTVEVEVRSDRGSQKFVFNGQPHPQNGF
                     LVKLVETARNNAGLVVPVPGADTLLQLAENAELGVYNLTELARQALEVNDLASAKRMA
                     EAALAQDPQNQAARKILARVTQVSQQVPAEQPAAPAPAQPAAPQAQAQEPQADLTLVG
                     PQAQAEVPGALAAQVAQDRQILAEAVSKEVQASINAARKLMGTNPETAREQLKLKLDE
                     VTRVPDLKPDVREQLLDQLRTAIRQADTRVVELNEQRRRAAQAEAQATERRLIAEGLL
                     LKEQKLSQLMARFNSLMDEGRYELAEVEAAGEAKKLSPDNPVPLLASHMASQVGDFEK
                     AMVLREKRQKNVLDTLYQTELAHVPIPDEPPILYPDAQVWQELTARRKERYQSMDLGS
                     QNPTERKILSELKKPTELDFVDTPLSEVVNYLKDRHQIEIELDQRALQDVGVDTNTPI
                     TKSLRGISLRSALRLLLRELDLTYVIKDEVLLITTPEQAGNDLVLKVYPVADLVVPPN
                     VMGGAGMGGWGGMGMGMGMMGGYGGYGMGGYGGYGMGGYGMGMGGYGMGMGGMWNVPN
                     NNGGFQAFDVKDDLSKSPTGEPRQAAKPSPVLALADQV"
BASE COUNT          423 a          704 c          562 g          472 t
ORIGIN      
        1 ccacttgatc ggctaaggca agaaccgggc taggcttggc agcctgtcgt ggctcgcccg
       61 tgggggattt gctcaaatca tccttgacat caaaggcctg gaacccgccg ttgttgttag
      121 gcacgttcca catcccaccc atgcccatgc cataaccacc catgcccatg ccgtagcccc
      181 ccatgccata gcccccgtag ccacccatgc catagccccc gtagccgccc atcatgccca
      241 tgcccatacc cataccgccc caaccgccca tgcctgcccc acccattacg ttgggaggta
      301 ctaccaggtc ggcgaccgga tagaccttca acaccaggtc attaccagct tgttcgggcg
      361 tggtaatcaa aaggacttca tctttgatta cgtatgtcaa atcaagttcc cgcaggagca
      421 aacgcagagc cgatcgcaac gaaatgcccc gcagactctt tgtgataggc gtgttggtat
      481 caacaccaac gtcctgaagg gctctctgat cgagttcaat ttcgatctgg tgccgatctt
      541 tcagataatt gaccacttcc gagagcggcg tatccacgaa atcgagttcc gtcggcttct
      601 tcaattcact caagattttc cgctccgtgg gattttggct ccccaaatcc atgctctgat
      661 accgttcctt ccttcgagcc gtcagctctt gccacacctg ggcatcggga taaaggatcg
      721 gaggttcatc aggaatggga acgtgagcta gctcggtttg atagagggta tcgagaacgt
      781 tcttttgccg cttttcgcga agcaccatgg ccttctcgaa atcacccact tggctggcca
      841 tgtgactcgc aagaagcggc accgggttat ccgggctgag ctttttggct tcaccggccg
      901 cctccacctc ggccaactcg tatcgaccct cgtccatgag ggaattgaag cgggccataa
      961 gttggctcaa cttctgttcc tttaggagca gaccttcggc aatcagacga cgctccgtgg
     1021 cttgggcttc tgcctgagcc gcccgccgac gctgttcatt cagctccacg acccgcgtat
     1081 cggcctgccg gatcgccgta cgaagctgat cgagaagctg ttcccgcacg tcgggcttaa
     1141 ggtcgggaac ccgggtgact tcgtccagct taagtttcaa ttgttcgcga gccgtctccg
     1201 gattggtgcc catcagcttt cgtgccgcgt tgatggaagc ctggacctcc ttgctgacgg
     1261 cttcagccaa aatctgccga tcttgggcaa cctgagccgc taacgcaccg ggtacctccg
     1321 cctgcgcttg cggacccacc agggtcaaat cggcctgcgg ctcttgcgcc tgtgcttgcg
     1381 gagccgcggg ctgggccggc gccggtgccg ccggctgctc cgccgggact tgctgcgaga
     1441 cttgcgtcac gcgggcgaga atcttgcggg cagcctggtt ttgtgggtct tgcgccaggg
     1501 ccgcctccgc catccgcttc gcggacgcca ggtcattcac ttccagtgcc tgcctcgcca
     1561 gctccgtaag gttgtaaacg ccgagttccg cattttcggc caattgaagc aatgtgtccg
     1621 cccctggaac gggcaccacc aatccggcgt tgtttcgagc cgtttctaca agctttacca
     1681 aaaacccatt ttgcgggtgg ggctggccat tgaacacaaa cttctgcgag ccacggtcag
     1741 accgaacctc gacttccacc gtgccgggat tcttcaaagt tcccacagcc accgtttccc
     1801 gatccgaacg aaggggcggc atccgtgaag gatagacctc tgccacttgc tccgaccacg
     1861 ctgcgccatt gggaaacgcc accgtcgctc gcacggcatc actcaaacgc tcgccaacgg
     1921 ctgccgcgtc caaatcgcca ctatccacga caagttgtcc tccggtatgc gtagctaacg
     1981 aggccaccag accgaaatcc acctgcgtcc caatgaggta ggcgctcaca ggaatcttgt
     2041 tatcgaccaa ccctgtgagg agctgcgaaa gcgtttctgc cgataagacg ttcgcgcggc
     2101 ttagtccatc gccaaaatac atcaggacgc gaccctctgc cggtgcttgc ttaagagctt
     2161 c
//
LOCUS       DAOPTR010000733         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_40005_length_2161_cov_2.525166, whole genome shotgun sequence.
ACCESSION   DAOPTR010000733 DAOPTR010000000
VERSION     DAOPTR010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40005_length_2161_cov_2.525166"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /gene="rlmN"
                     /locus_tag="PKI05_12650"
     CDS             <1..1225
                     /gene="rlmN"
                     /locus_tag="PKI05_12650"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOA53094.1"
                     /translation="SKGQPGYRVAQVLHWVYESPKISFDEMSDVPKVVRRELKKSFKI
                     FSMQVASHRKGSDGTEKLLLRTQDGEFVECVLLRDDKGNRTVCISTQIGCGVGCAFCA
                     SGLDGIVRNLTSGEILEQILQLKCLLQPEERLSHIVVMGMGEPLANLGRLLGALHRAT
                     HKDGLGISARHVTISTVGLPNGIRRLAELNPQYHLAVSLHAPNDELRNRLVPANRRIG
                     IQAILQAADDYFAKTGRRVTYEYVLLRNVNDRPEHAQELAELLRGRNALVNLIPYNPV
                     QGLPYEVPDRRDVGRFADILSRMGIQVTIRYRKGSEIEAACGQLRRVHPEIVSKSKKK
                     KKKKSAAAGQEPTGSSESSPTSDNTSTSDSVIISNDATVAISDHELQMYEATEDLPAE
                     AEQAAPAKPSDQVSE"
     gene            complement(1217..>2161)
                     /gene="ruvB"
                     /locus_tag="PKI05_12655"
     CDS             complement(1217..>2161)
                     /gene="ruvB"
                     /locus_tag="PKI05_12655"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOA53095.1"
                     /translation="MEEMVGQRDVYERLQIAIDAALKRDEPLGHILFDGPPGLGKTTF
                     ALCIPRHLGVSVQIASGATLKAPKDLIPYLTNAEERSVLFIDEIHRMQKSVEEFLYPA
                     MEDFRIDITLGEGVNARTLNIRLKPFTLIGATTRSGLLSAPLRDRFHMREHLDFYTVD
                     ELAEIVRRNAQKLQIKIDPNAAEEIARRSRGTPRIANNRLRWVRDYATSRANGRITLQ
                     VAEDALAMQGVDKLGLDVQDRKYLETIARVFGGGPVGVEAVAHTMNLSPDTLIDEVEP
                     FLLRSELVVRTPRGRKLTPKAYEHLGMSYPTPNQGYLF"
BASE COUNT          465 a          604 c          620 g          472 t
ORIGIN      
        1 gagcaaggga caaccaggtt atcgggttgc tcaagttctc cactgggttt acgaatcgcc
       61 caagatttcc tttgacgaga tgtcggacgt tccgaaggtt gtccggcgag agctgaaaaa
      121 gtcgttcaag attttcagta tgcaggtagc gagccatcgc aaaggctccg atggcaccga
      181 gaagttgctc ttgcgaaccc aggacggtga attcgttgaa tgcgtcttgc tccgcgacga
      241 caaaggaaac cgcacggtgt gcatcagcac gcagattggt tgcggtgtcg ggtgcgcgtt
      301 ctgcgccagt ggcctggatg gtatcgtccg gaacttgacc tcgggggaaa ttctcgagca
      361 gattttgcaa ctcaagtgtt tgctccagcc ggaagagcga ctcagccata tcgtggtgat
      421 ggggatgggc gaacccctgg cgaacctggg acggctcctg ggggccttgc atcgggcaac
      481 gcacaaagat ggattgggga tcagcgcacg ccacgtcacc atttcgacgg tagggcttcc
      541 caacggtatc cgacgacttg ccgagttaaa cccacagtac catctggccg tctcgttgca
      601 cgcgcctaat gatgaactcc gcaatcggct ggttcccgcc aatcgtcgga ttgggattca
      661 ggccatcctt caggcggccg atgactactt tgccaaaact gggcggcgtg tgacatacga
      721 gtatgtcttg ctgcgcaacg tgaacgatcg tccggaacat gcccaggagt tggccgagct
      781 tttgcggggc cgaaacgccc tggttaacct gatcccgtat aatccggtcc agggtctccc
      841 ttatgaggtt cctgatcgtc gcgacgtcgg ccgttttgcc gatatcttga gtcggatggg
      901 cattcaggtc acgattcgtt accggaaggg ttccgagatt gaggcggcct gcggccaact
      961 gcggcgcgtc catcccgaga tcgtcagcaa gtccaagaaa aagaagaaaa agaagagcgc
     1021 cgcggccggt caggaaccaa ccggttcatc agaatcgagt ccgacctccg ataatacttc
     1081 cacatcggac tccgtgatca tctcgaatga cgcgacggtc gcaatttccg atcacgagct
     1141 acagatgtat gaggcgaccg aagacctgcc tgccgaagcc gagcaggccg ctccggcgaa
     1201 gccgtctgac caggtctcag aatagatagc cttggttcgg agtaggataa ctcattccta
     1261 agtgttcgta agccttcggc gtaagtttgc gcccacgagg tgttcgcaca accaattccg
     1321 atcggagtaa gaacggttcc acctcgtcga taagtgtgtc cggagagaga ttcatcgtgt
     1381 gggcaacggc ttccactccg accgggccac cgccaaagac gcgcgcgatg gtttccagat
     1441 acttacggtc ctggacgtct agccctagct tatcgacacc ttgcatggcc agggcgtctt
     1501 cggcaacctg cagcgtaatt cgcccatttg cccgacttgt ggcatagtcc cgtacccacc
     1561 gcaggcggtt gttggcaatt cgcggggtac cgcggctgcg acgagcaatt tcctcggcgg
     1621 cgttgggatc gatcttaatc tgcagctttt gggcgttccg ccgcacgatt tcagccagtt
     1681 catctacggt atagaaatcg agatgctccc gcatgtggaa acggtcccgc agtggggccg
     1741 aaagaaggcc tgaccgcgta gttgccccaa ttaacgtaaa tggcttcagt cggatgttga
     1801 gggttcgcgc attgacgcct tcccccaagg tgatgtcgat ccgaaagtcc tccatcgccg
     1861 gatagagaaa ttcctccacc gatttctgca tccggtgaat ctcgtcgatg aaaaggacgg
     1921 atcgctcttc ggcattcgtc agatagggga tcaagtcctt aggagccttg agcgtggccc
     1981 cactggcgat ctgaacgctg acccccaaat gccgcggaat acacaaagca aatgtggttt
     2041 tgcccagccc gggtggtccg tcaaagagga tatgtcccag cggttcgtct cgtttcaggg
     2101 ctgcgtcaat cgctatctgc aatcgttcgt acacatcgcg ctgaccgacc atttcctcca
     2161 t
//
LOCUS       DAOPTR010000734         2152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_40218_length_2152_cov_2.836433, whole genome shotgun sequence.
ACCESSION   DAOPTR010000734 DAOPTR010000000
VERSION     DAOPTR010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40218_length_2152_cov_2.836433"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="PKI05_12660"
     CDS             <1..1125
                     /locus_tag="PKI05_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53096.1"
                     /translation="HYQRRSGLTKSSIVSFNHFLRTQAEEATLFEKVGPFARTADSPV
                     CFQPGTYTCAGRSLPGVRLHPPNGQDRYTFCRVEVQVPSEGNPRLEAAVGVVELPTLS
                     DGCGFQVVALSGDKLTHLMETFQRPGPWKEIRVPLGPFAGQRINLEFRTDPGPKGQST
                     ADWAVWADPRIVVESDHGEKVIWRLMDHLETTVSGARRAGRPFVYNDQTIALYSGLPL
                     IKGQVVTTPYFVWAMHHRLAHGELRVNLPGRNGILDDDTGGLTATADENRIVLLLWHF
                     DVMQDSPRSWQIRLKKMPPAMSQAKQFRCTEYRIDREHNNPYTRYVRRGEDSQGGAYN
                     LETASLEVTSKSLVRTDAGTASLEVVLPNMAVSLIEICPE"
     gene            1243..>2152
                     /locus_tag="PKI05_12665"
     CDS             1243..>2152
                     /locus_tag="PKI05_12665"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOA53097.1"
                     /translation="MKRIIVIVGMMFSLLSLMQADGQQRPREMPREDVVDVPAISEGL
                     CVSNLFQTNMVLQRDKPINIWGWANPGEKVTVSFAGQEVTTQAREDRSWQVTLNPIPA
                     NSTPQTMTIRGADRTITLENILVGDVWLLGGQSNMEFPIASVDDGELEIVSANSPQIR
                     LLTVPQGKGFDSVRSFERLYEWSSWSNRHFRKGDWDVCSPETVREFSAIGYVFGRRLH
                     MATGVPIGLIDTSIGGTTVETWTPLEVLEQIEGRETREMLEDWNARIAAYDPQADLQA
                     RIAAYEASIEARKERGEPIPPNSAPPS"
BASE COUNT          480 a          599 c          625 g          448 t
ORIGIN      
        1 cactatcagc ggcgatcagg cctcacgaag tccagcatcg tcagtttcaa tcactttctg
       61 cggacgcagg cagaagaggc gacactcttt gaaaaggtgg ggccttttgc gcgaactgcc
      121 gacagccccg tgtgctttca gccgggaacc tatacgtgcg cggggcgctc tttgcccggc
      181 gtgcgactcc atccccctaa cggccaggat cgctacacct tctgccgcgt ggaggtacag
      241 gtaccatctg aaggcaatcc ccgattggag gccgctgtgg gcgtggttga gttgcccacg
      301 ctaagtgacg gctgcgggtt tcaggtggtg gccctttcag gcgacaagtt gacgcatctg
      361 atggagacat ttcagcgccc cggtccctgg aaggaaatcc gtgtgccact cggcccgttc
      421 gccggacagc ggattaacct cgagtttcgc accgatcccg ggcccaaggg ccaatcaaca
      481 gccgattggg cggtctgggc agacccccgc atcgtcgtgg agagcgatca tggggagaag
      541 gtcatttggc gactgatgga tcaccttgag acgaccgtat ccggtgcccg ccgggcaggc
      601 cgtccgttcg tgtacaacga ccagaccatc gccctttata gtggtctgcc tctgattaag
      661 ggtcaagttg tcaccacgcc ctatttcgtt tgggcaatgc atcaccgact tgcccacgga
      721 gagctccgtg tcaacctgcc cggtcgtaat ggtatcctgg acgatgatac gggcggattg
      781 acggccacgg ctgatgaaaa ccgcattgtg ctactcctct ggcattttga cgtgatgcaa
      841 gactcacctc ggagttggca aatccgcctg aaaaaaatgc cgcccgccat gtcgcaggca
      901 aaacaattcc gctgcacgga gtaccgtatc gaccgtgaac acaataatcc ctacacccgt
      961 tacgtacgac gcggtgagga ttcccaaggc ggggcctata atctcgagac ggcgtcgctg
     1021 gaagtcacat ccaaaagcct ggttcggacg gatgccggca cggcatcgct ggaggttgtc
     1081 cttcccaaca tggccgtctc gttaatcgag atatgccccg aataaagtgc gatcagaatg
     1141 atgagacaca cagcgttttc cgtactatgg agacatttcg gtgcatttct ttgataggta
     1201 aagcttgagg gttcaaacat ttcgagacac agggacgcat ctatgaaacg aatcattgtt
     1261 atcgtgggaa tgatgttcag tctgctcagt ctgatgcaag ccgatggaca gcaaaggcca
     1321 cgagagatgc cgcgggaaga tgtggtcgat gttccggcca tcagcgaagg gctatgcgtg
     1381 agcaatctct ttcaaaccaa catggtgctc cagcgcgata agcccatcaa tatctgggga
     1441 tgggcaaatc cgggggaaaa ggtgacggtc tcgtttgcgg gccaagaggt caccacacag
     1501 gcgagggaag atcgatcgtg gcaggtcaca ttgaatccta tccccgctaa ttcgacaccg
     1561 caaacgatga cgatccgcgg tgccgatcgc accatcacgc tggaaaacat cttggtgggc
     1621 gatgtctggc ttttgggtgg ccaaagcaat atggaattcc ctatcgccag tgtggatgac
     1681 ggcgaactgg agatcgtctc ggccaattct cctcagattc gcctgttgac cgtacctcag
     1741 ggaaaaggct ttgattcggt tcggagcttt gaacgcctct acgagtggag ttcgtggtcg
     1801 aaccgacact tccgcaaagg cgactgggat gtctgttcgc cggaaactgt gcgagagttt
     1861 tcggccatcg gttacgtttt tggccgccgc ctacacatgg ccacaggcgt gcccatcggc
     1921 cttatcgaca catccattgg aggaaccacc gtggagacgt ggacaccttt ggaagtgttg
     1981 gaacaaattg agggtcgcga aacacgggag atgctggagg actggaacgc acgaattgcc
     2041 gcctacgatc cccaagccga tttgcaagcc cgcatcgctg cctatgaggc ctccattgag
     2101 gcccggaagg aacgcggaga gccgatccct ccgaacagcg ccccaccgag cg
//
LOCUS       DAOPTR010000735         2147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_40320_length_2147_cov_3.846558, whole genome shotgun sequence.
ACCESSION   DAOPTR010000735 DAOPTR010000000
VERSION     DAOPTR010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40320_length_2147_cov_3.846558"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PKI05_12670"
     CDS             <1..293
                     /locus_tag="PKI05_12670"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOA53098.1"
                     /translation="LGVDVPPARFVEAIRQHQPCVVGMSLLLTTCYSSVTATVEAIRQ
                     AGLRDQVAIMVGGAAASELLAKNAGCDFYGKTAVDGLKFACQVAGITQPTSP"
     gene            655..1926
                     /locus_tag="PKI05_12675"
     CDS             655..1926
                     /locus_tag="PKI05_12675"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOA53099.1"
                     /translation="MQNVLTIVLGGGRGTRLYPLTKFRSKPAVPIAGKYRLIDIPLSN
                     CINSDLSRIYVLTQFNSASLHRHIRATYNFDVFHRGFVEILAAQQTLTSSDWYQGTAD
                     AVRQNLAYINRDDVDYVLILSGDQLYRMDFRELIAAHEKKGADVTIAALPVMKRAASS
                     LGIMRIDEHGQVLGFLEKPQTEEELRYMQTDPDWLSQFGIQAEGREYLANMGIYLFSR
                     KTLVDVLEMTDYRDFGKEVFPASIRTRRVFVYLFDGYWEDIGTIGAFFEANLALTKPN
                     AAFSLEHPNAPIYTRARFLPPSRIESATIQSSLVADGCIIGSGSVIENSIIGLRSVIG
                     RNCVIRNSIIMGNDFYDLEQTRDGKPPMIIGEGSYIENAIVDKNCRIGKNARIVNTTG
                     LTEMPENQYCMIRDGVVVIPKETILPDKWCL"
     gene            1961..>2147
                     /locus_tag="PKI05_12680"
     CDS             1961..>2147
                     /locus_tag="PKI05_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53100.1"
                     /translation="MTRIKLSRPVPWQDVVFALGLVLGLLAAQYATGDIPGDIAILLS
                     SGQSTTKPEDVSEAWKRL"
BASE COUNT          478 a          595 c          593 g          481 t
ORIGIN      
        1 acttaggcgt cgatgtcccg ccggcccgat ttgtggaggc cattcgccaa catcagcctt
       61 gtgtcgtggg gatgagtctc cttttgacaa cgtgctattc atccgtgacg gccacggtgg
      121 aggccatccg ccaagctggt ttgcgagacc aggtggcgat catggtgggc ggggcagccg
      181 ccagtgaact gctagccaag aacgcgggct gtgatttcta cgggaagacg gccgtcgatg
      241 gattgaaatt cgcctgccaa gtcgctggca ttacccagcc caccagtcct taagcccagt
      301 gtcggccacg acttagagcg tatcgcaaaa actcctcctc tccctctggg agagggttgg
      361 ggtgagggca agccatcgtc ggaatcgact gagaaaaccg gcagcaattc gaaccaccct
      421 caccctcgac ccctctcccg ccgaacgggc gagggtggga ttatggaata gacttttaaa
      481 gtcttccttt ttctccgtcg ctagtcacgc atcatgcgaa ttcaccgggg aacggtcgcc
      541 ttctgcaaac acggtcgcct cgcccgccaa gacttgcatc gggaatgatt tcggctagta
      601 tgggcagaag gcgtcaacga gctgagattg ggttctagcg tgtaagggaa gcctatgcaa
      661 aacgtcctga ccatcgttct gggaggtggt cgtggcacac gactctatcc actgacgaag
      721 tttcgttcca aaccggcggt accgattgcc gggaaatatc ggctcattga catccctctt
      781 tccaattgca tcaacagcga cttgagtcgc atttatgtcc tcacccaatt caattcggca
      841 agtctccatc gccatatccg agcgacgtac aatttcgacg tcttccatcg gggattcgtg
      901 gaaatcctgg cggcccagca gacattgacc agctccgatt ggtatcaggg aacggcggat
      961 gccgtccggc agaatcttgc gtatatcaat cgggatgatg tggactatgt gctaattctt
     1021 tcgggcgatc aactgtatcg catggacttc cgggagctca ttgcggccca cgagaaaaag
     1081 ggcgcagatg tgacgatcgc cgccctgccc gtgatgaaac gcgccgcatc aagtctgggg
     1141 atcatgcgga ttgacgagca cggccaggtc ctcggcttcc tcgaaaaacc tcaaaccgaa
     1201 gaagaattgc ggtacatgca aaccgatccc gattggctga gccaattcgg catccaggcg
     1261 gaggggcgtg aatacctggc caatatgggg atttacctct tttcccgcaa gacactggtc
     1321 gatgtcctcg aaatgacgga ttaccgcgat ttcggaaaag aggtatttcc ggcgtcgatt
     1381 cgcactcggc gggtcttcgt ttatctgttc gacggttatt gggaagatat tggaacgatt
     1441 ggagcctttt tcgaggcgaa cctcgcgctg acaaaaccca acgccgcgtt ttcgctggaa
     1501 caccccaacg cccccattta cacacgagcg aggtttttgc ccccatcccg cattgagtcg
     1561 gccaccattc agtctagttt ggtcgccgat ggctgtatca ttggcagcgg gtcggtcatt
     1621 gagaatagca tcatcggtct gcggagcgtc attggtcgca actgcgtcat tcgcaactcg
     1681 atcatcatgg gcaacgactt ctacgatctt gagcagaccc gcgatggcaa acctcccatg
     1741 atcatcggcg aaggctctta cattgagaat gccatcgtgg ataagaattg ccgcatcggc
     1801 aagaatgccc gcatcgtcaa cacaacgggg ctgaccgaaa tgccggaaaa ccagtattgc
     1861 atgattcgcg acggcgtggt cgtgatcccc aaagaaacca tcttgcccga taaatggtgt
     1921 ctgtgatccc atgttgccga ggttcacaga gaggcatccc atgacccgaa tcaaactgtc
     1981 tcgtcccgtt ccctggcagg acgtggtatt tgcactgggg ttggttttgg gattactcgc
     2041 tgcgcaatat gcgacaggcg atatcccagg ggacatcgct atcctattaa gcagcggtca
     2101 gtccaccacg aagcctgagg acgtctcgga ggcctggaag cgtttgg
//
LOCUS       DAOPTR010000736         2143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_40425_length_2143_cov_2.536398, whole genome shotgun sequence.
ACCESSION   DAOPTR010000736 DAOPTR010000000
VERSION     DAOPTR010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40425_length_2143_cov_2.536398"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            901..>2143
                     /locus_tag="PKI05_12685"
     CDS             901..>2143
                     /locus_tag="PKI05_12685"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA53101.1"
                     /translation="MIEFAIALACAGCLAAVETFLVGVQAWEHRRFAASRLRSSPKAQ
                     MQAAGRAMVIIPCRGIEPNLKGNLERFFRQDYSNYGLRFVVESHDDPAAEVIQQLIEE
                     YPNVPAELVTAGLANGEAQKIHNLRVATEKIPPEVEYLVFADSDAAPSEHWLTAMLTR
                     LGDPKVSAVTGYRWFIPKTGAFHEAVVCALNGAYAMLMSRNTPNLVWGGSWAIRKEVF
                     EELDIRGAWRGRICDDLVAADRLLKCGRHVEYEPACLVATSCQPDLKAVFEFASRQFF
                     LLRRVLPLWWFTDLVVGYLGTVVFWLAIVTLIMVPDTLVRWTSAIVAAVSYALHWARA
                     ELRVAAVRTYFPRLAGEASFRRVFWYDRVLSPAIQLIGLLASLNGGLRRQIIWRGIIY
                     RMGKKAEIEIVRPSNGSSAYSE"
BASE COUNT          406 a          547 c          642 g          548 t
ORIGIN      
        1 tgtcctttgg atgcgcctat ttacattcat ctgacaccca tcacgagtta ttttatgcgg
       61 aaatcaacgg gcatcaagcc cccttcggac gaacaatttg gcaaatccag gcaagttccc
      121 ttgcggcaat ccttcaccct gtggcacagg ctgtctagga caaacccgga tacgaggcct
      181 tgcaccctgc tcaggaggct ctttgccgac ttggtggacg ccactgacag tttctgagca
      241 atccagcact gctttttggg gaagaggtga accgcgcgat cctgggttcg acatctcggc
      301 gttgcgtgct ctccgggcta ttgcagttca gagtgttaac ggcagcgcgt tagtcccgcc
      361 agaaaaggag gccttgtgtg tagcgcgttt tctgttgccg gcggatacgt tgcgattttt
      421 tcttattggc atcttttatg gcagtctatc tcgttgcggg ttgtccactt gcgcgacgtc
      481 gcccttccaa ttctggagta ctttcttcat ttgcacgatg ttctggggtg aacttgcggc
      541 caacgccagg tgcgagttgc gtggccttgt gttcggtgtg ccttgctgct gagacgcctt
      601 ctgtaaactc cgcccacgaa atgtctagct acaagattga tgcacacggc atcatggtgt
      661 cgtttgtatg cgaaggcaat atttgccatt gaccggacga gctaaacggg ctattttgac
      721 gcgaaggggg aatgtatgcg cgaacgggta acatgaataa tttggctggt tcggtggcct
      781 ttgcgcgagc gtaaggttat ttcgagggct tcgcggggca agatggtttg agtcccaacg
      841 tgaaactttc tgtcgcaatg gatgcctctc ttgttcctcc agcgggaagg attcccctca
      901 atgattgagt ttgctatcgc tttggcttgt gcgggatgcc ttgccgcggt tgagactttt
      961 ctcgtcgggg tccaggcgtg ggagcatcgc cggttcgccg ccagtcgatt gcggtcctcg
     1021 cccaaggctc agatgcaggc cgctggccgc gcgatggtca ttatcccgtg ccggggaatc
     1081 gagccgaacc tgaaaggcaa ccttgaaaga tttttccggc aagattacag caactacggc
     1141 ctgcggtttg tcgtggaaag tcacgatgac cctgccgctg aggtcattca gcagttaatc
     1201 gaagagtacc cgaatgtgcc tgccgagctt gttacagccg gtttggccaa cggtgaggcg
     1261 caaaagatcc acaatcttcg tgtggcaacg gagaagattc ctccggaagt agagtacctt
     1321 gttttcgctg attcagacgc agccccgtcg gagcactggt tgaccgctat gctgacgcgg
     1381 ctgggcgacc caaaggtctc cgcggtgacg ggctaccggt ggtttattcc caagactggg
     1441 gccttccacg aagccgttgt gtgtgccttg aacggggcat atgcgatgct catgagccgg
     1501 aataccccga atctcgtatg gggaggatct tgggcaatcc gaaaagaagt ttttgaggag
     1561 cttgacatcc ggggggcatg gcggggcagg atctgtgacg acctggtggc tgcagatcgg
     1621 ctcctcaaat gtggccggca tgtggaatac gagccggcat gtctggttgc cacatcgtgc
     1681 cagccggacc tcaaggccgt gtttgaattt gcctcccggc agttctttct cttgcgacgg
     1741 gttctccccc tttggtggtt cacggaccta gtggtcgggt atctgggcac ggttgtgttt
     1801 tggctggcga ttgtcacact catcatggta cctgacactt tggtccgatg gacctcggcg
     1861 attgtggctg ccgtatcgta tgcgcttcac tgggcgcggg cggagctgcg ggtggctgca
     1921 gtgagaactt attttccccg tttggccggt gaggcaagtt ttcgacgggt tttttggtac
     1981 gatcgagtac tcagtcccgc cattcaattg ataggcctcc tagcgagctt gaacggaggc
     2041 ttgcgacgtc agattatttg gcgaggcatc atttaccgaa tggggaaaaa ggccgaaatc
     2101 gaaattgtcc gacccagcaa cggctcctca gcgtactcgg aga
//
LOCUS       DAOPTR010000737         2142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_40441_length_2142_cov_4.058457, whole genome shotgun sequence.
ACCESSION   DAOPTR010000737 DAOPTR010000000
VERSION     DAOPTR010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40441_length_2142_cov_4.058457"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1423)
                     /locus_tag="PKI05_12690"
     CDS             complement(<1..1423)
                     /locus_tag="PKI05_12690"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOA53102.1"
                     /translation="MHSPLMMTWRNIWIVFAREIRDQLRDRRTIFMVAVLPVLLYPLL
                     GMIFFQLAQVMGEKPSRILVIGAREPLEPPLFDETGFVSSVFVSPQDAVLLKVEFLSD
                     EVLKARAGSASEDPGRALAEEALRTEHYDAVLYFPPGFAAQLADFREGIRQQIAAGLS
                     SEANSEAKPGSEKSPEEAGEDASQKQQPTPRTVVVSRSAIPSPRVYYSTANDRSQTAY
                     RRLVELLDRWTDQVMVANLAAARLPPDAARPFVFETADVAVQSGFRGAAVWAKILPIL
                     LVMWALTGAFYPAVDLCAGEKERGTLETLLSSPARRSEIVLGKLGAITVFSIVTAVLN
                     LISMGLTGWLAFGHLPDFAAPPLFAIFWLAVALLPVSLVFSCLALGIAAFARSTKEGQ
                     YYLLPLLFCTLPLVGVPMARGSELDLGTALVPITGLVLLLKTMIEGQMTEALRFVFPV
                     GLVTLGGCYFALRWAVYQFNSETV"
     gene            complement(1451..>2142)
                     /locus_tag="PKI05_12695"
     CDS             complement(1451..>2142)
                     /locus_tag="PKI05_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOA53103.1"
                     /translation="ISFSASPGEIFGLLGPNGAGKTTTLRILSTLLKPTAGRVLINGY
                     DGLTQPDQVRRSIGFMSVNTAVYDRMTAWEMVEYFGMLYGLDRQELRDRMERLFDRLK
                     MQEIRDLLGSKMSTGMKQKVSIARALVHDPPVLIFDEPTAGLDVLVARALLTTIAELR
                     DQGKCVIFSTHIMREAEKLCDRIAILHRGQILAMGTLEELREQSGENDLEELFFQLIT
                     EAERKRADRAS"
BASE COUNT          525 a          646 c          547 g          424 t
ORIGIN      
        1 gaaccgtctc gctgttgaat tgataaaccg cccaacgcag ggcaaagtag catccgccta
       61 aggttaccag tcctacaggg aacacaaagc gtagtgcctc cgtcatttga ccttcgatca
      121 tcgtcttgag gagaaggacc aaccctgtga tgggcacaag ggcagtgccc aaatccaatt
      181 ccgatccccg cgccatcggc acacccacca ggggcaaggt gcaaaacaaa aggggcaaaa
      241 ggtaatattg accctctttg gtactgcggg cgaacgcggc aatgcccaag gccaagcagc
      301 taaacaccaa cgagacgggc agtaatgcta cggccaacca aaagatggca aaaaggggcg
      361 gtgcggcaaa gtccggcaag tgcccaaagg caagccagcc ggtcaaaccc atgctaatca
      421 ggttaagcac ggcggttaca atgctgaaaa ccgtgatggc gcccagtttt ccgaggacaa
      481 tttcactccg ccgagccgga ctcgaaagga gcgtttccaa cgtcccgcgt tccttttctc
      541 cggcacaaag atcgacggcc gggtaaaaag cccccgtcaa tgcccacatg accagcagta
      601 taggcagaat ttttgcccac accgccgcac cgcgaaaacc gctctgaact gccacatccg
      661 cggtttcaaa aacaaacggt cgcgcggcat ctggagggag cctagctgct gccaaattcg
      721 cgaccatgac ctgatccgtc cagcgatcga ggagctccac caatcggcga taggccgttt
      781 gtgaccgatc gtttgccgtg gagtaataca ccctcggact aggaatcgcc gaacgactga
      841 caacaaccgt tcgtggcgtg ggctgttgtt tttgggaagc gtcttcccct gcctcttccg
      901 gactcttttc agaaccgggt tttgcttcgg aattggcctc ggacgacaga ccggccgcaa
      961 tctgttgccg aattccctcc cgaaagtcgg ccaactgtgc cgcaaaacca ggaggaaaat
     1021 aaagcacggc atcataatgc tccgtgcgaa gggcctcttc ggcgagcgca cgtcccggat
     1081 cttcggaggc ggatcccgcc cttgccttta gcacctcgtc ggagaggaat tccactttca
     1141 ggagaaccgc atcttgaggc gaaacgaaca cactgctgac aaaacccgtt tcgtcaaaca
     1201 aaggtggctc cagtggttct ctagccccta tgacgaggat gcgactgggc ttctcgccca
     1261 tcacctgggc aagctggaaa aatatcatgc ccaacaaagg gtaaagcaaa actggcagga
     1321 ccgccaccat aaaaatggtc cgccggtccc tgagttgatc gcggatttcg cgggcaaaga
     1381 caatccagat attgcgccag gtcatcatca gtgggctatg catctttccg ccccgggcgt
     1441 caatccgtcc tcacgacgcc cggtcggctc gttttctttc agcttcggtg atcagttgaa
     1501 agaacaattc ctccaagtca ttctctccgg attgctctcg caattcctcc agggtaccca
     1561 tggccagaat ctgtccccga tgcaaaatag cgatgcgatc gcacagcttt tccgcttccc
     1621 gcataatgtg cgtggaaaaa atgacacatt tcccctggtc gcggagttcc gcaatagtgg
     1681 tcaggagtgc gcgggcaacc aagacgtcca atcccgccgt tggctcgtcg aagatcaaca
     1741 ccgggggatc atggacaagg gcccgggcga tggacacctt ttgcttcatc cccgtggaca
     1801 tctttgaacc taacaggtcc cgaatctctt gcatcttcag tcgatcaaaa aggcgttcca
     1861 tccgatcccg cagctcttgc cgatccagcc cgtacagcat cccaaagtat tcgaccattt
     1921 cccaggccgt catgcggtcg taaaccgctg tattgaccga cataaaaccg atggagcgtc
     1981 gcacctgatc gggctgcgtc agtccatcgt aaccgttgat gagtacccga cctgcggttg
     2041 gcttcaaaag cgtactaaga atccgcaatg tggtcgtctt acccgccccg ttcggcccga
     2101 gaagaccgaa gatttcccct ggtgacgcag aaaaactgat cc
//
LOCUS       DAOPTR010000738         2140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_40489_length_2140_cov_4.182734, whole genome shotgun sequence.
ACCESSION   DAOPTR010000738 DAOPTR010000000
VERSION     DAOPTR010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40489_length_2140_cov_4.182734"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            623..1867
                     /locus_tag="PKI05_12700"
     CDS             623..1867
                     /locus_tag="PKI05_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53104.1"
                     /translation="MNSQVRFEIVVWVSIAVLSVCVSGHLQAGQPSPNAGANWGLEAS
                     SLDTSDQWPAGTWDRAASHGDPVPVQGTAPCCEICGAGYDCPRLWTWYNGAKILNRGR
                     ARSALLTVNPAGEGLLNVDNANFDVAGGYETRVQYFLKNDPENRDQFIEFVYWGLNEW
                     KDQQSVNGQRLTYQSSLFGGTSFEAGSLFTFYDFVDFNYADLHRIRYESEINNFELNW
                     WFKPRPRPDRLALLPNGKWQRQCQPGVYFNYAFGFRGLSLDEGFIFDSRGVVDAQGGP
                     ETLYGRQTIRSKNDTFGLQLAGELLFRRHRFEWGARVKAAPMFNWAEQTTDIALAHNP
                     GHVVRYAKDDDVCFMGEVSIVANYRIFPRMRAFASWDMMWLDGVALAPEQLDFGLKSP
                     GVINDNGTVYFQGVSLGFDVVW"
BASE COUNT          439 a          541 c          626 g          534 t
ORIGIN      
        1 ccagctcttg ccatccttgg cgagtttcca tggcttgtcc tctcgttgaa accgttccgc
       61 gaaggatact cagacgaatt ttagaatttt ttcttgtctt cgcaggcaac gcatctctcg
      121 cagagcgcgc atatgacttc acatgccgga aagccgccat accgcggcat ctatatgact
      181 tctcgcatgc tctgcaaaca ttgaagattg tgtagattgt cctctttgac atcgacattg
      241 agatgtgcgg acttaaaaac tgggattgat gtgcagctta tgatgactag gaaaaatgcg
      301 gcggctcgtt gtcttgtagg tgatgagggt accacaccga tctactaacc tgcgtagcta
      361 gacgaagata aggcaaatgg aggggcggga tagacaacag aggttggtct gaggctttct
      421 catccaccgg gcgggcatgc tgctgcgagg ctggaacgcc gaatgtttcc caatttgccg
      481 gatatgacgt ctgggaagaa tttgtcgatt atttctgttt ttccggcctc ctttagccga
      541 tgacccattt gctgcgtctc tacactttct caaccttggg tccctctctg gagtctttgc
      601 accgagaaac ggagggaaac gcgtgaacag ccaagttcgt ttcgaaatcg tcgtctgggt
      661 gtccattgcg gttttgtccg tgtgtgtgag cggtcatttg caagccgggc agcctagccc
      721 caacgcgggg gcgaattggg gcttggaagc atcttcgctc gacacgtcgg accaatggcc
      781 ggcggggacc tgggatcggg ctgcctccca cggtgacccg gtgccggtcc agggtacggc
      841 gccttgttgc gagatttgtg gtgccggata cgattgccca agactttgga cctggtacaa
      901 cggcgccaag atcctaaatc ggggacgtgc ccgatcggcg ttgctcacgg ttaacccggc
      961 aggagagggt ctccttaacg tggataacgc caattttgat gtggccggcg ggtacgagac
     1021 ccgcgtccag tatttcctaa aaaacgaccc agagaaccgc gatcagttca tcgagttcgt
     1081 ctattgggga ctgaacgaat ggaaggatca gcaatcggtt aacggccagc ggctcaccta
     1141 tcagtcgagc ctcttcggcg ggaccagctt cgaggccggt agtttgttca cgttttatga
     1201 ctttgtggac ttcaattacg ctgatctcca ccggattcgt tacgagtccg aaatcaacaa
     1261 ctttgagttg aattggtggt tcaaaccccg gccgcggcct gatcggttgg ccctgttacc
     1321 caatggaaaa tggcagcgtc aatgtcagcc gggcgtgtac ttcaattatg cctttggttt
     1381 ccgcggccta tcgctggatg agggattcat cttcgatagt cgaggtgtcg ttgacgcgca
     1441 gggtggcccc gaaacccttt acggccgtca gacgatccgt tctaagaatg atacctttgg
     1501 tctgcaattg gccggcgagc tattgttccg gcgtcatcga ttcgaatggg gtgcccgagt
     1561 caaggcggcg cccatgttca attgggccga gcaaaccacc gacatcgccc ttgcccacaa
     1621 ccctggccat gttgttcgct acgccaaaga cgatgacgtg tgttttatgg gcgaggtaag
     1681 cattgtggca aattatcgaa tctttcctcg gatgcgggca tttgccagtt gggatatgat
     1741 gtggttggat ggtgtggctt tggcgcccga gcaactcgat ttcggcctga aaagccctgg
     1801 cgtgatcaac gacaacggga cggtttactt ccagggtgtc tcgctggggt ttgacgtggt
     1861 gtggtagtca aaagtcagga gaccgtggta aatttgctcg ggtggggtag gttgagatcc
     1921 ccatcacgct atgtcggctt gcctttggca gcatgtgcgc acaaggccac aacgagccac
     1981 cggttccggt cttgtacgta atgaccgcca gcgcggatgg ttgagaacag tcgggacggc
     2041 ctcatggaaa cgcgcggtca aattgggcgg ttaaggacca tggggcacgg taatgtcctc
     2101 gtccttttac tcttaaccgc agtcttcagg gcagtcgttc
//
LOCUS       DAOPTR010000739         2138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_40543_length_2138_cov_3.680749, whole genome shotgun sequence.
ACCESSION   DAOPTR010000739 DAOPTR010000000
VERSION     DAOPTR010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40543_length_2138_cov_3.680749"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PKI05_12705"
     CDS             complement(<1..475)
                     /locus_tag="PKI05_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53105.1"
                     /translation="MRRFSLLAMVILGFAISCLCAEEPSHSTSFRIATFRCDVTPPPD
                     GHPLIWLVPVKEVETPLEARGVVLDDGQRRYVLCAMDWCGVCNSTYEMFRSALAEGAG
                     TSPDDVALQTVHQHTAPYTDAGAQPFLDEVKSGIRYVDMDFLGIVATRLKESVKES"
     gene            1015..1683
                     /gene="purN"
                     /locus_tag="PKI05_12710"
     CDS             1015..1683
                     /gene="purN"
                     /locus_tag="PKI05_12710"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOA53106.1"
                     /translation="MPNESSVTKGHPFRIAVLISGGGTTLKNLIEKVKSGELPVSIVL
                     VISSNPQAKGLQYAEEEGIPAIVVDWNQYRDSNSFSAVIFKHCQEARANLIVMAGFLK
                     KLVIPNEYENRVVNIHPSLIPAFCGQGFYGYRVHQAVLDYGAKISGCTVHFADNLYDH
                     GPIILQRTVPVLDDDTPETLAKRVAAEEFKAYPEAIRLIAEGRVQVEGRKVRILPPPT
                     ESEL"
     gene            1790..>2138
                     /locus_tag="PKI05_12715"
     CDS             1790..>2138
                     /locus_tag="PKI05_12715"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOA53107.1"
                     /translation="MPLDPTLHPDWEIALEAESRMKTCYQLADELGLTRDELLPYGHY
                     IAKVDYLKVMERLKDGPDGKFIEVTGITPTPLGEGKSTTTIGLLQGIGKRKRRVTACI
                     RQPSGGPTMNIKGS"
BASE COUNT          514 a          561 c          582 g          481 t
ORIGIN      
        1 gcgactcttt cacagattcc tttagccggg tcgccacaat ccccagaaaa tccatgtcaa
       61 cataacgaat accggattta acctcatcca aaaatggctg cgcaccagcg tctgtgtaag
      121 gtgccgtgtg ttggtggact gtttgtagcg caacgtcatc gggacttgtg cccgcccctt
      181 ctgcaagggc actgcgaaac atttcgtaag ttgaattgca tacgccacac cagtccatag
      241 cgcatagcac atatcgcctt tgaccgtcat ctaagacaac acctctcgct tcaaggggag
      301 tctccacctc tttgacgggg accagccaga ttaaggggtg tccatcgggc ggcggtgtca
      361 catcgcagcg aaacgtggcg attcggaaag atgttgaatg tgacggctct tcagcacata
      421 ggcagctaat ggcaaagccc agaatgacca tggccaaaag tgagaaacgt cgcatcgttt
      481 ttcctccgct cggctcggca tcagacgata agccaaggcc ttcccgtcaa cccgacatta
      541 gtatatccag accgttctga gtgtccacag gagcagcggg agcgaagccg gtcgtgattt
      601 gacttccact catgcgaagt gcggccacga caatcggtgg tcttccattg gatcgggcag
      661 acactaggcc tgcccctcct agcgggtgga aaacgcacgt ccgattagaa aacaccacca
      721 tctcaagatg ggcgctcacc ttacagccac gccatctcgc ggtcttccat cataaggcct
      781 acctacggca tgacgcccaa ctttccattg tggtgtgggc ttatcggtcc ctcgggcagt
      841 atcttaacca catgcgccgt ttcattttgg ttgtgcgagc gtcttcggaa ggcgaggtta
      901 accgcacgga ggcagttctg aaggagcatt gtcagacgtt gacgcggtca ttagagtgaa
      961 ttacaaatcc ttgctaataa cgctggtgcg agttgacgag ataggagatg tgcgatgcca
     1021 aatgagtcgt cggtgacaaa gggccaccct tttcggattg cggtccttat ttccggaggc
     1081 ggaacaacac ttaagaacct gattgaaaag gtgaagtcag gcgaattgcc cgtttcgatt
     1141 gtcctggtca tatcgagcaa tcctcaggcg aaagggctac aatacgctga ggaagaaggg
     1201 attccggcga tcgttgtcga ttggaaccaa tatcgagatt ccaactcgtt cagtgccgtc
     1261 atctttaaac attgccagga ggcgcgagct aaccttatcg taatggctgg atttttaaag
     1321 aagcttgtca tccctaatga gtatgaaaat cgggtcgtga atattcatcc ctcactgatt
     1381 cccgccttct gcgggcaggg cttttacgga tacagggttc accaggctgt gttagattac
     1441 ggagctaaaa tcagcggatg taccgtgcac tttgcagata atctttacga ccacggtcca
     1501 atcatcctcc agcggaccgt gccggtcctg gacgacgaca cgccggagac ccttgccaag
     1561 cgcgtcgctg cggaggagtt caaggcctac ccagaagcca tccgcttgat tgcggaaggt
     1621 cgtgtgcaag tggaagggcg caaagtccgc atcttgcccc cgcccacgga atcagaactt
     1681 tgatgagcga caccacaggc gtgtctgtgc acttgttctt tgccgagtta tattagtccg
     1741 ccaagtgtcg acaccgatcg ttaccgtttt agctgctgtg ggaaatcgta tgccattgga
     1801 tccaacgctt catcccgact gggagattgc cctggaagcg gagtcccgaa tgaagacgtg
     1861 ttatcagctc gcggacgagc tgggattaac acgtgatgag ctattgccgt acgggcacta
     1921 tatcgccaag gtcgattatt tgaaggtcat ggaaaggctc aaggatggcc cggacggcaa
     1981 gttcatcgag gtcacaggta ttacgccaac gccgttgggt gaaggcaaat cgaccacgac
     2041 catcgggtta ctgcagggga ttgggaaaag gaagcgacgg gtgacagcgt gcattcggca
     2101 accgtcgggc ggtccaacca tgaacatcaa aggctcgg
//
LOCUS       DAOPTR010000740         2137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_40570_length_2137_cov_4.484150, whole genome shotgun sequence.
ACCESSION   DAOPTR010000740 DAOPTR010000000
VERSION     DAOPTR010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40570_length_2137_cov_4.484150"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PKI05_12720"
     CDS             complement(<1..513)
                     /locus_tag="PKI05_12720"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOA53108.1"
                     /translation="MNQYWLGSTIIVFGFCVGGWSIGISSAVGHESPIACQADAPLCS
                     PSPQAQAKRWTWEGVEMAAPLRIVCYHSDERQAARAMQKAVERIRELNNVFSDYDAGS
                     EVRRLCDTAGSGRPIPVSEDLWRLLELSLRISQETDGAFDVTVGPLSRLWRRARMSKE
                     MPPAWRFDEAR"
     gene            1096..>2137
                     /locus_tag="PKI05_12725"
     CDS             1096..>2137
                     /locus_tag="PKI05_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOA53109.1"
                     /translation="MSRKGWSHWLILGLVVGSLIAAYGWAQAEDAASNKPDPALEPVI
                     KWLPEPYPVPDADAKTEAEMKPYTEVIVGTDIKFDMVPIPGGRFIMGSPENEKGRKPD
                     EGPQHEVEIEPFWMAKYETLWDAYETWSFELDKRRREQTKEPATKWDEIADAIARPTP
                     PYTDMSFGMGKKDRPAVCMTLYAAQHFCKWLSAKTGRYYRLPTEAEWEYACRAGTKTA
                     YSFGDNVDDLEDYAWFFDNADDKYHKVGEKKPNPWGLYDMHGNVAEWVLDQYVPDAYK
                     QFAGKVVKNPLVPATKEFPIVVRGGSWMDDPEMLRSAARRGSHPDWKMQDPQIPQSIW
                     YLTDAPFVGFRVV"
BASE COUNT          472 a          586 c          632 g          447 t
ORIGIN      
        1 tctcgcctcg tcaaagcgcc aggccggcgg catttcttta gacatgcggg cacgtcgcca
       61 aagccgactg agcggtccca ccgttacgtc aaaggctccg tctgtttcct gagaaatgcg
      121 gaggctcaac tccagaagcc gccagaggtc ttcactgacg gggatgggcc ttccagaccc
      181 ggccgtgtcg cacagtcgcc ggacctcgct accggcgtcg taatcgctaa aaacgttgtt
      241 gagctcacga attcgctcga cggccttttg catggcccgg gcggcttgcc gttcgtcgct
      301 gtgatagcac acaatccgaa ggggggctgc catttccacg ccttcccagg tccaccgctt
      361 cgcctgcgct tgcggtgacg gcgagcagag cggggcatcc gcttggcaag cgatcgggct
      421 ctcatggcca accgccgagg aaatcccgat cgaccaacct ccaacacaaa acccaaaaac
      481 aataatcgtg ctgccaagcc aatattgatt cattttgcaa gacctcaatt ggtggtcaga
      541 tcgtaagaaa ggctgtccaa cggggcggag gcatgaccca tacttagagc ctatcccatg
      601 atctcccctc acccgttggg cgggacaggg ggcgggggtg agggtggctt gaatcgctgc
      661 cggttttctc aatcgattcg attctgacgt tcgattgccc tcaccccaac cctctcccgg
      721 agggagaggg ggactttttg ggatagactc ttattccccc tagggccgac acatgccggg
      781 tgccgttctg atgacctcac gcctttgcga ggagagcggg gcagtttccg gagattccaa
      841 cccgtttgcg tccctgggaa acaaagttta caataccagt tattatcatg gggagttgca
      901 atcagggaaa ggttggcggg tcgggcagga gtctcccggg gcatcataag tgaggcattt
      961 aaccaggctt tgacaacggg gcacaaagcg agaaatgagc tttgagttac tcccctagag
     1021 gatgctggct agattgcaaa ggcgatagtg gtcggtttgt cccttgaacc caattgatca
     1081 atgaggtgac gttgcatgag tcgaaaaggt tggagtcact ggttaattct gggattggtg
     1141 gtagggtcgt tgattgcggc gtatgggtgg gcgcaagccg aggacgcagc ctccaacaag
     1201 cctgatccgg cgctggagcc cgtgatcaag tggcttcccg agccgtatcc tgtccccgac
     1261 gccgacgcca agaccgaagc cgagatgaag ccttacaccg aggtcatcgt gggaaccgac
     1321 atcaagttcg acatggtccc gatccccggt ggcaggttca tcatgggcag cccggagaat
     1381 gaaaaaggac gtaagcccga cgaagggcct cagcatgagg ttgagattga gcctttctgg
     1441 atggccaaat atgaaacgct gtgggatgcc tatgaaacat ggtccttcga attggataag
     1501 cgccgtcggg agcaaaccaa agaaccggct acgaagtggg atgaaatcgc agacgccatt
     1561 gcccgtccca cgccgcctta tactgacatg tcctttggga tgggcaagaa ggaccgcccc
     1621 gccgtttgca tgacgttgta cgcggcccag catttctgca agtggctctc cgccaagacg
     1681 ggccgatatt accgcttgcc cacggaggcc gaatgggaat acgcctgtcg cgcaggtacc
     1741 aagacggctt attccttcgg cgacaacgtg gatgacctcg aagattatgc ctggttcttc
     1801 gataacgccg acgacaagta tcacaaggtt ggggaaaaga agcccaatcc ttgggggctt
     1861 tatgacatgc acggcaatgt cgcggaatgg gtcctcgatc aatatgtgcc ggacgcctac
     1921 aaacagtttg ccggcaaggt ggtgaagaat cctttggttc cggcaacgaa ggaattcccc
     1981 atcgtcgtgc gcggcgggtc ctggatggat gaccctgaaa tgttgcggag cgccgcccgc
     2041 cgaggttcgc atcctgattg gaaaatgcag gacccccaga ttccacaaag tatctggtat
     2101 ctgacggatg ccccgttcgt cggttttcgt gtggttc
//
LOCUS       DAOPTR010000741         2137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_40573_length_2137_cov_4.376561, whole genome shotgun sequence.
ACCESSION   DAOPTR010000741 DAOPTR010000000
VERSION     DAOPTR010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40573_length_2137_cov_4.376561"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PKI05_12730"
     CDS             complement(<1..182)
                     /locus_tag="PKI05_12730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOA53110.1"
                     /translation="MSKRVLIADDSVLIRRMIAEVLTQAGYEVVGEATNGAEAVAKYR
                     ELNPDVVTLDIEMPGM"
     gene            complement(404..610)
                     /locus_tag="PKI05_12735"
     CDS             complement(404..610)
                     /locus_tag="PKI05_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53111.1"
                     /translation="MSSAVRIALGEKLRDGFHKLWLDTGPTNRIMMEASHVADMPHKW
                     ADAVFADCNGNTLPNTLWLDAGVR"
     gene            655..948
                     /locus_tag="PKI05_12740"
     CDS             655..948
                     /locus_tag="PKI05_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53112.1"
                     /translation="MELNRYHYYMLGLFLLLLGLELRTIEAVVLTPEVTKFLAERTGH
                     PVATASNAVAAVTGTSPPIPPKTIRPPEWLGYAVLSAGSVLVLYSLALPKPGG"
     gene            1039..1506
                     /locus_tag="PKI05_12745"
     CDS             1039..1506
                     /locus_tag="PKI05_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53113.1"
                     /translation="MFSTTNVCRRKLGQVLASVTLAGIVRSLFAMEQASLPSENATAN
                     APDSGREGKSAEKSAPTRPRLREGTKLFDLVGTVRQTGRRYTFFVAANSNRFVLLENL
                     MLERILRAQASHGVTIQWKISGTVTEFQGQNYLLIEHALLHASSQEEPKPNVP"
     gene            1716..>2137
                     /locus_tag="PKI05_12750"
     CDS             1716..>2137
                     /locus_tag="PKI05_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOA53114.1"
                     /translation="MQHSEQRRTVNRRSFLKASAIGALGATAAFRFAHGALNEDFQVK
                     IGLIGCGGRGTGAVLNALGAGTRVVYPAKGYHTEDVAEDASVTRKDVQVTALADLFPD
                     RLESCRANLAKLGINVPPERCFVGFDAYKQLLALDDVT"
BASE COUNT          493 a          605 c          546 g          493 t
ORIGIN      
        1 ttcatgccgg gcatttcgat gtctagcgtc actacatcgg gattcaactc gcggtacttt
       61 gccacggctt cggcaccgtt tgtggcttca ccgaccacct cgtagccggc ctgggtgagg
      121 acctcggcaa tcatccgtcg aatcagcact gagtcgtcgg caattaaaac cctcttggac
      181 atgattgact cctaggcagt tctgtgcacg ctcccagatg atcctaccgt cgcacattaa
      241 gttaacgtgg ccgatccaat ctgcttcgta gcttggtttc tggaacacaa cagccgacct
      301 atgcgaaccg ttcactaaaa cagtaggtca caaatcggcg atctgccaca aatccgcgca
      361 ttgtagagtt ggccgcttgc aatgctttgc attgtctttt gaattatcgc acgcccgcat
      421 cgagccacag cgtattaggc agcgtattcc cattgcaatc cgcaaaaacg gcatcggccc
      481 acttatgcgg catatccgcc acgtgcgaag cctccatcat aatccgattg gtaggtcccg
      541 tatctaacca cagtttgtga aatccatcac gcagcttctc acctaacgca atccgcacag
      601 ccgatgacaa aaaagggaac tgcccctggt gtcaagacaa aggcattttc aaccatggaa
      661 ttgaaccgct atcactacta catgttgggg ttgtttcttc ttttgttggg gctggagctc
      721 cggaccatcg aggccgtagt cctaaccccc gaggtaacga agttcctggc agaacgaact
      781 ggtcaccccg tggccaccgc cagcaatgcc gttgcagcgg tcacaggcac gtccccacca
      841 attccgccca agaccatccg cccaccggaa tggctcggtt atgcggtcct ctcagccgga
      901 tcggtcttgg tcttgtatag ccttgccttg cccaagccag gcggttagca agaaaagaac
      961 accggctagt cctggcccag cttcatttcc gtcagagcgg gggcactcgt aacctatcca
     1021 cggggagagg gaagctcgat gttttcgacc accaacgttt gcaggcggaa acttggccaa
     1081 gtcttggcca gcgtcacgct cgccggaata gtacggtcgc tctttgcaat ggaacaggcg
     1141 tcattaccat ccgagaacgc cactgctaat gcacccgatt ccggccgaga aggtaaatcg
     1201 gcagaaaaat ccgcgccgac tcgcccccgg ttgcgagaag gaacaaaact ctttgacctc
     1261 gttggcaccg ttaggcaaac cggtcggcgt tacacattct ttgttgctgc caatagtaat
     1321 cgctttgtcc tgttggaaaa ccttatgctg gaaaggattc tccgagcgca ggccagtcac
     1381 ggcgtcacaa tccaatggaa gatttccggc actgtgaccg agtttcaggg acaaaactac
     1441 ctgctcatcg agcatgccct tttgcatgcg agttcccaag aagagcccaa gccgaacgtt
     1501 ccgtgagggg aaaacctcat gcaacctcac gggaggtttc cttacatctt ttcgaaacta
     1561 cgtctcgcct cgggggcgtc tgtctggcac ggtcgctaaa acatttcgaa acgccccggt
     1621 ccaataaacc agttgcttac cgccaacgtc atgcgtatct ttgattcttg ctcgatgtaa
     1681 caaccagaag gaacagttct ttcaggagat cggtcatgca acactcagag cagcggcgaa
     1741 cggttaatag gcgatccttc ttgaaagcgt ccgcgattgg ggccttggga gccacagcgg
     1801 catttcggtt tgcccatggt gctttgaatg aggattttca agtaaaaatt ggcctgattg
     1861 gctgtggtgg acggggaaca ggtgcggtcc tcaacgccct tggggccgga acaagggttg
     1921 tttatcccgc aaagggttac catacggagg acgtggctga ggatgcctcc gtcacccgga
     1981 aggacgttca ggtgaccgca ttggctgact tgtttccgga tcgtctcgaa tcatgtcggg
     2041 ccaatctagc aaaattggga atcaatgtcc cgccggaacg gtgttttgtg ggctttgatg
     2101 cttacaaaca actccttgcc ctagacgatg tgacata
//
LOCUS       DAOPTR010000742         2134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_40641_length_2134_cov_3.632035, whole genome shotgun sequence.
ACCESSION   DAOPTR010000742 DAOPTR010000000
VERSION     DAOPTR010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40641_length_2134_cov_3.632035"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            344..1651
                     /locus_tag="PKI05_12755"
     CDS             344..1651
                     /locus_tag="PKI05_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOA53115.1"
                     /translation="MVWFYERSSHIDWARIEELMVRAVDEARERICRHPKRVLLLPPD
                     ITRMHSGAGRMTEIIYQILSRDADVHVIPTLGQHEPHSREQNARMFGSIPHERIHAHH
                     WINTVVRLGEVPANFVEQVTHGAANWAIPIELNSMLMQEPWDLVINIGHVVPHEVLGF
                     ANHNKNYFIGLAGKGTICASHIAAACCGIENNLGTLITPVRACYNKAENEFLGSLPDF
                     YIQIVMARDPEDRLVHTGIYVGDDLETYLMAARQSREENIKVFEEPLQKVVCVMQEDE
                     FVSTWVANKAIYRTRMAMADGGELIIIAPGLKRFGEQEEVDRVIRKYGYVGTQRVMEL
                     YRQNQDLQELAHATAHLIHGSSEGRFRITYAPGHLTKEEIEGVNFGYADLQETLRRYP
                     PDKMREGWNTTPDGETIYFIPTPSAGLWATRKKLYERPAGYEC"
     gene            1800..>2134
                     /locus_tag="PKI05_12760"
     CDS             1800..>2134
                     /locus_tag="PKI05_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53116.1"
                     /translation="MDISRREGKPTARPPAKGDDKPPVVKKRRVFSLSHVGEGLVLLI
                     ALAPVFLIGAVAGSKSLWTNWLEKIGVRESWGNETSHVPPAAVWQGDGVADFGQIAVY
                     TYDPASGLVR"
BASE COUNT          480 a          573 c          594 g          487 t
ORIGIN      
        1 tcttcgggaa cactcatgga cctaaatctt ttaaattttc ttcccccgtc gggcaaaggc
       61 ccggtcaagc gttgggcaga tagtgcccca ttcgcttggt cacatgtccg aagtgtccag
      121 gccaagctgg accgtctctg atgacagccg tttccgaccg tgccccgccc tactttcagg
      181 taccctcgaa aagacctctc aaatccgcga taatgtcgtt tttatttacc gcagggtggc
      241 agagctagtc ggccatcccg ggcgtacaaa aagtaaggac aattgaccgt atttcaagag
      301 ctcggccaaa catcgacaac acgtcactgg caagaggttg cacatggtct ggttttatga
      361 acgatcgtct catattgatt gggcaagaat cgaggaattg atggtgcggg ccgtggacga
      421 ggcccgagag agaatctgcc gccatccgaa acgagtgctc cttctgcccc cggatatcac
      481 gcggatgcac tctggtgccg gtcgcatgac ggagatcatc tatcagattt tgtcacgcga
      541 cgccgatgtc catgtcattc ccacgctcgg tcagcatgag ccgcacagcc gggaacaaaa
      601 cgcgcggatg ttcggttcca ttccccatga gagaatccac gcccaccact ggattaacac
      661 ggtcgtccgc cttggcgaag ttccagccaa tttcgttgaa caggtcaccc acggggcggc
      721 caactgggcc attccgattg aactgaatag catgctcatg caagaaccct gggacttggt
      781 gatcaatatc ggacatgtcg taccgcatga ggtgttgggg ttcgccaatc acaacaagaa
      841 ttacttcatc ggtttggctg gaaaagggac catctgcgcc tcgcacatcg ccgcggcttg
      901 ttgtggtatt gagaataacc tcggcacgct gatcacgccg gttcgtgctt gttataacaa
      961 ggcggaaaac gagttcctcg gttctcttcc agatttttat atccagattg tgatggcccg
     1021 cgatccggaa gatcggctcg tgcatacggg catctacgtg ggtgacgact tggaaaccta
     1081 tcttatggca gcccggcaat cgcgtgagga gaacatcaag gtcttcgagg agcctctcca
     1141 gaaagtcgtg tgcgtcatgc aggaagatga gttcgttagc acgtgggttg ccaataaggc
     1201 gatctatcgt acccgcatgg cgatggccga tggcggcgag cttatcatca tcgcaccggg
     1261 attgaagcga ttcggcgaac aggaggaagt cgatcgggtc attcgcaagt acggatatgt
     1321 gggaactcag cgtgttatgg agctgtatcg tcaaaatcaa gacctccaag agctggccca
     1381 cgccacggcc catcttattc atggttcttc cgaggggaga tttcgaatca cctacgcgcc
     1441 cgggcacctc accaaagaag agatcgaggg cgtcaacttt ggctatgctg acctccagga
     1501 gaccctgcgt cgatatccgc ccgacaaaat gcgcgagggc tggaacacca cccccgatgg
     1561 ggagaccatc tacttcattc ctacgccgtc ggccggttta tgggcaacgc ggaagaagct
     1621 ttacgagcgt cctgccggat acgagtgtta gtctcactcc ttgacgcatc aatggttcaa
     1681 ttgtttcgaa ccggatgcac cgaattgccg gagcgtgatc cgagtttgtg agctaggatt
     1741 gggatagatt cactcttccc ttatctcggt ccggccagtt tctattgcga ggttctcctt
     1801 tggatatttc tcggagagaa ggaaagccca cggcaaggcc gccagcaaag ggtgatgaca
     1861 aaccgccggt ggtcaagaaa cggcgtgtgt tctcgctttc tcacgtgggt gagggactcg
     1921 tcttgcttat cgccctcgcc ccggttttcc tcattggagc cgtggcaggt tccaagagtc
     1981 tttggaccaa ttggctggaa aagataggcg tccgtgaaag ctggggaaac gaaacaagcc
     2041 acgttccgcc tgccgctgtt tggcaaggag acggggttgc cgactttgga caaattgcgg
     2101 tttacactta cgacccggcg tcgggacttg tccg
//
LOCUS       DAOPTR010000743         2133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_40656_length_2133_cov_4.036574, whole genome shotgun sequence.
ACCESSION   DAOPTR010000743 DAOPTR010000000
VERSION     DAOPTR010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40656_length_2133_cov_4.036574"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(416..>2133)
                     /locus_tag="PKI05_12765"
     CDS             complement(416..>2133)
                     /locus_tag="PKI05_12765"
                     /inference="COORDINATES: protein motif:HMM:NF028477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4965 domain-containing protein"
                     /protein_id="HOA53117.1"
                     /translation="ALLSRPVTYLIWDVRSLDGKPRIVDLYFDATGELAVNEPNQLVT
                     WHRGQTEHLDWMRIGTTEQPILGKKGDNLRIDWGYAYVAAVRHPQTNTVVADHETARN
                     HFCNTGRIPRRDDGNKPRAVQDRWPVLVCMHSAKVSRDESLKWHILLAYDDIVSIEYL
                     GQRLRPYWRQRYADISEALAAAEEELPDLMARCQAFDEELLADARSLGGVGYANLIAI
                     SYRHTLGAHKLTLGPHGEPMYFSKECFSNGCIGTVDVIYPAAPIFMLFNNELLKATVV
                     PVLDYAASPRWKFPFAPHDLGTYPLANGQVYGGGETSEEHQMPVEESANLLILAYAIS
                     RLDGNTAFADRYTKLWEQWAHYLEEKGFDPENQLCTDDFTGPLAHNCNLSIKATIALA
                     CYAEMCHMSGKIEEANKYRKLAEEFAQKWIAAADDGDHYRLAFDKPGTWSQKYNLVWD
                     KLFGLNIFPPVVRQKEIAFYKKQLQPYGLPLDNRALFTKTDWTVWTATLADSREDFDA
                     LMNPLYRFIDHTPDRVPLTDWYWTHDAKLRGFRARPVIGGVFIKFLESPDLWKKWATR
                     CARMK"
BASE COUNT          448 a          575 c          622 g          488 t
ORIGIN      
        1 tcaactccga cgttcgcttg ccctcacccc aaccctctcc cggagggaga gggggacctt
       61 ttgggatagg ctctgacgac tttgtgggct aggcgaagag gagggacggc gtccccgccg
      121 tccggtgatt tggtcattga ggccggtggc cctgccatta cgggtgtttt ttgggaagat
      181 cctccgttcc ctctgaaggg tcacaatgac gtcttgccca agtgacaccc atatggagtg
      241 tttgggcaag gcgcgacgtc cttttcacag aagaacgtct gccgccgatt cttttcctgg
      301 gtgacgtgat agagcagccc ccccaatgac caaacttacg aacctcgcca tggcgagaca
      361 ctgccaagtc caagccgttg ggtaaaaatc gggttatgcc tgcggaatcg gggcatcact
      421 tcatgcgcgc acagcgagtg gcccactttt tccaaaggtc cggcgattcg aggaatttga
      481 taaacacccc accgatgaca ggccgggctc ggaatccccg tagcttcgca tcgtgggtcc
      541 agtaccaatc ggtcagcggg acgcggtcgg gcgtgtgatc aatgaaacgg tagagcgggt
      601 tcatgagggc gtcaaagtct tctcgagaat ccgccaatgt ggccgtccaa accgtccagt
      661 ccgtcttcgt aaaaagggca cgattatcaa ggggcagccc ataaggctgg agttgcttct
      721 tgtagaacgc gatttccttt tggcggacca caggtgggaa gatgttgagg ccgaatagtt
      781 tgtcccaaac gaggttgtat ttctggctcc atgtccccgg cttgtcgaag gccagtcgat
      841 agtggtcacc atcgtcagcg gccgcgatcc atttttgggc gaactcctcg gcaagcttgc
      901 gatacttgtt tgcctcctcg atttttccac tcatgtgaca catttccgcg taacatgcca
      961 gagcaatggt ggcttttata gaaagattgc aattgtgggc aagggggccg gtgaaatcat
     1021 ccgtgcagag ctgattttca ggatcgaaac ctttctcctc cagataatgt gcccattgtt
     1081 cccacagttt tgtatatcgg tcggcgaagg ccgtatttcc gtcgaggcgg gaaatggcgt
     1141 aggcgagaat gagcaagttc gccgattctt cgaccggcat ttggtgttcc tcgctggtct
     1201 ctccgccacc gtaaacctga ccattggcca ggggataggt tcccaaatcg tgtggcgcaa
     1261 aagggaactt ccatcgcggg ctagcggcgt aatcgaggac ggggacgacc gtggctttca
     1321 gcaactcgtt gttaaaaagc atgaagatgg gtgctgcggg ataaatcaca tccacggtcc
     1381 cgatgcagcc attgctgaag cattccttgg agaagtacat cggttcgccg tgtggcccca
     1441 gggtcagttt gtgcgcaccc agggtatgcc ggtaggaaat cgcaatcaga ttggcatagc
     1501 ccacgccacc gaggcttcga gcgtccgcga gaagttcctc atcaaaggcc tggcatcgag
     1561 ccatgaggtc tggtagctct tcctcggccg cggccagggc ttccgaaatg tctgcgtatc
     1621 gctgacgcca atagggtcgc aagcgttgcc ccaggtactc gatggaaaca atatcgtcgt
     1681 aagccagcaa gatgtgccat ttgagtgatt cgtcgcgact taccttggcc gagtgcatac
     1741 ataccaacac aggccatcgg tcctggactg cccgaggttt gtttccgtcg tcacggcgcg
     1801 ggattcgccc ggtgttacaa aaatgattac gggcggtctc gtgatcggct acaaccgtgt
     1861 tcgtttgggg atggcgcacg gcggcgacat aggcgtagcc ccaatcgatc cttagattgt
     1921 cgcccttttt cccgaggatc ggttgctccg ttgtaccgat gcgcatccaa tccaggtgct
     1981 cggtctggcc gcggtgccag gtcacaagct gattcggctc gttgacggcc aactcgcctg
     2041 tggcgtcaaa atagagatcc acgatccgtg gtttgccatc gagtgagcga acatcccaaa
     2101 taagataggt gacaggccgg gaaaggagtg cca
//
LOCUS       DAOPTR010000744         2132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_40696_length_2132_cov_2.937410, whole genome shotgun sequence.
ACCESSION   DAOPTR010000744 DAOPTR010000000
VERSION     DAOPTR010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40696_length_2132_cov_2.937410"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2132
                     /locus_tag="PKI05_12770"
     CDS             <1..>2132
                     /locus_tag="PKI05_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53118.1"
                     /translation="RITVIPDQPPTVEIVKPTSQSSAAIGDKVPIRVRASDDYGLGRI
                     RLEMKIQQADADGAKGSSDEAEPLKLQDWDNPPGPFVDYELVLSPERFQPGQVVLLRA
                     VAWDKRNLGKFGLDLRPQKAESPWVMIRLISTETKTTEELKTLENLRAAIFRILEEQI
                     RAKGALVTLTRTIEDNPGQFSGGIHAIRARQVEIRSSTEAVVKSILDTDSEERKIIRR
                     VLSNLVANEMLQAVQECDRLVKLSDLSKAKDEMVPLGQHQDQIIETLRKILDAARIAQ
                     TELLKEAEKRAGGDLPDDVKKKLEEIHSKLQEFLEQQKKVIEASEELSKKPVEDFTEK
                     EEQILKELAETQDDWSRFMKEAHTDLSKLPEQDFANATLCQELNEIQTELKMAEDALL
                     KKSVDIAVPLEQLGYEMAKELTTNLEKWLPDTPDRERWSQEESLSDQDKEAPMAELPG
                     QLEDLIGELMEQEEDLFDEMEDISSSAADSLDKGAGWDAADGPISNMSAKGVTGNRLP
                     NTSEIGGRSGEGRSGKSSGEFVSEEAVGKGGRKTPSRLTPDPYMKGQIKDFSKDPTGG
                     ATGGGKESGQGGQGLEGPTPPPHGPRELQRLAGKQAELRNKAESINAQMQVLNYHHTD
                     MEQLIELMKEIERDLLAGRYQNALRKREVVLQGLRTQKEYVGGEFEVKQDATLNIPKD
                     VQKEILGSVQEPAPAGWEEIVRGYYRQL"
BASE COUNT          589 a          491 c          650 g          402 t
ORIGIN      
        1 atcgcattac ggtgattccg gaccagccgc cgaccgtgga gattgttaaa ccaaccagcc
       61 agtcttcagc agcgataggc gacaaggtac ctatcagggt ccgcgcatcc gatgactatg
      121 gtctggggcg aatcaggtta gaaatgaaga tccaacaagc ggatgccgac ggagctaaag
      181 ggagttcgga cgaagccgag ccactcaaac ttcaagactg ggataacccg cccgggcctt
      241 tcgtcgatta cgaattggtc ctttcgccgg agcggttcca accgggtcaa gttgtcctcc
      301 tccgtgcggt ggcttgggac aagcgcaatc tgggcaagtt tgggttggat ttgcgaccgc
      361 aaaaggcgga atccccttgg gtcatgatcc ggctgatttc gacggaaacc aaaacgacag
      421 aggaacttaa gacactcgaa aacttgaggg ctgccatctt ccgcatttta gaagaacaga
      481 ttcgagctaa gggagcattg gtgactctga caaggacgat tgaggataat cccggacagt
      541 tttccggagg gattcacgcg atccgggcgc gacaagttga gattcgcagc agcacggagg
      601 ccgtggtcaa gtcgattctg gacacggatt cagaggagcg aaaaatcatt aggcgggtgc
      661 taagtaatct cgttgccaat gaaatgctcc aggcggtaca ggaatgcgat cggctggtca
      721 aactctctga cctctcaaag gctaaggacg aaatggtgcc tttagggcag catcaagatc
      781 agatcatcga gacattgcga aagattcttg atgccgcgcg tatagcccag acagagctcc
      841 tcaaagaggc cgagaaacga gcgggtggtg atctgcctga tgacgtcaag aagaaactgg
      901 aggagattca tagcaagctc caggagttct tagaacaaca gaagaaggtc atcgaggcct
      961 cggaagaatt gtctaagaaa cctgtcgaag actttaccga aaaggaggaa cagatcctta
     1021 aggagttggc cgagacgcag gatgattggt cccgcttcat gaaagaggcc catacagacc
     1081 ttagcaagct gcctgagcaa gactttgcca acgccacact ttgtcaggag cttaacgaaa
     1141 ttcagacgga attgaagatg gccgaggatg ccttattgaa gaaatcagtt gacatcgctg
     1201 ttccgcttga acagctcggc tacgagatgg ctaaagagct gacgactaac ttggaaaagt
     1261 ggttgcctga tacgcccgat cgggagaggt ggagtcaaga agagtcattg agtgaccagg
     1321 ataaggaagc accgatggcg gaacttcctg gtcagctcga agatttgatc ggcgaactaa
     1381 tggaacagga ggaagacctc tttgacgaga tggaggatat ctcgtcaagt gctgcggact
     1441 cgttagacaa aggggcgggc tgggatgctg cagatggtcc gatctcgaat atgagcgcga
     1501 aaggcgtcac gggcaaccga ttacccaaca caagtgaaat cggtggacgt tcgggtgagg
     1561 gtcgcagcgg caaatcgagc ggtgagttcg tcagcgagga ggctgtgggc aaaggaggtc
     1621 ggaaaacgcc aagtcgcttg acccctgatc cctatatgaa ggggcaaatt aaggatttca
     1681 gtaaggatcc gacaggtggc gcaacgggcg ggggaaagga aagtggccaa ggcgggcagg
     1741 gactcgaggg tccaacaccg ccgccccatg gtccgaggga gttgcaacga ctggccggca
     1801 agcaggctga gcttcgcaat aaggcagaat ctatcaacgc ccaaatgcag gtcctcaact
     1861 accatcacac ggatatggag caactgatcg agctaatgaa ggaaattgag cgggatctgt
     1921 tggccggacg ataccaaaat gccctccgta agcgcgaggt cgtcttgcag ggattgcgga
     1981 cccaaaagga gtacgtgggc ggtgaattcg aggtgaaaca agatgcgact ttgaatatcc
     2041 ccaaggatgt ccagaaggaa atcctgggaa gtgttcaaga accggccccg gccgggtggg
     2101 aggaaattgt ccgcggatac taccgacaac tg
//
LOCUS       DAOPTR010000745         2127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_40825_length_2127_cov_2.867761, whole genome shotgun sequence.
ACCESSION   DAOPTR010000745 DAOPTR010000000
VERSION     DAOPTR010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40825_length_2127_cov_2.867761"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..533)
                     /locus_tag="PKI05_12775"
     CDS             complement(159..533)
                     /locus_tag="PKI05_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53119.1"
                     /translation="MHPSLLVSVIVGDVLFILGFLAFFFGGAEHPTALIPALFGAVIQ
                     TLAIIAHFFPKTRGPFAKVILIVAFVGLVIGGARPAMVLLGGGQVSWTLALVSQAGLA
                     LGCAVVFCASIVLLLGQAFQKS"
     gene            complement(569..1717)
                     /gene="carA"
                     /locus_tag="PKI05_12780"
     CDS             complement(569..1717)
                     /gene="carA"
                     /locus_tag="PKI05_12780"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOA53120.1"
                     /translation="MPKPAKLALEDGTVYRGYSFGAEGVVDGEVCFNTSMTGYQEILT
                     DPSDRGQIVVMTYPEIGNYGVNEEDFESWKPHLAGFVVREVSCRVSNRRAQENLDSFL
                     KRFGIVGLYGIDTRALVRRIREHGAMKGVLSTVDLDDASLVAKAKASPGLVGRDLVRE
                     VLPPGPREWAQPLSRWAKLGGQVRERIPPDAPHVVALDFGMKYNIARHLFDSGCRVTV
                     LPGTATAEEVMSFRPDGVFLSNGPGDPEPVQYAQKTIAQLLGKVPIFGICLGHQLLAL
                     ACGARTYKLKFGHRGANQPVMNKETGRVEITCQNHGFAVDPDTLPPYLEVTHWNLNDD
                     TVEGLVHREVPAFSVQYHPEASAGPHDSHYLFGRFRKLIDESRASLAG"
BASE COUNT          491 a          618 c          588 g          430 t
ORIGIN      
        1 attatgaagt aaggacatcg caaggatatg gccgcggcga cattggcggg taggggtcac
       61 cgttgtggcc gcccgccgct caatctcaca caatggatct cgcaacacag ttccgccggt
      121 ccaaagcgat gagaggtcag gacctttgca aattgggact acgatttttg gaaggcctga
      181 cccagcaaaa ggacgatgct ggcacagaag accaccgcgc atcccaacgc caatcctgcc
      241 tgggaaacaa gagccaaggt ccaactgact tggccgcccc ctaggagaac catggcagga
      301 cgtgcgcccc cgatcaccag acccacaaag gcaacgatga ggataacctt ggcaaatggg
      361 ccgcgggtct tgggaaagaa gtgagcgatg atggccaagg tctgaatgac ggctccgaac
      421 aaggccggga tcaaggccgt tgggtgttcg gccccaccga aaaagaacgc caaaaagccg
      481 agaataaaca ggacgtcccc gacgatcaca ctcaccagca gtgacggatg catggtcgcg
      541 ctccccctcg agaggtctca ccgggacgtc agccggccaa cgacgctcgc gactcgtcga
      601 ttaacttccg aaaacggcca aagagatagt gactatcgtg gggcccggct gaggcctccg
      661 ggtgatactg gacactaaat gcggggactt ctcgatgtac gaggccctcg acggtgtcgt
      721 cgttgagatt ccagtgggtg acttcgagat aggggggcag ggtgtcggga tcaacggcaa
      781 agccatggtt ctggcaagtg atttctaccc gcccggtctc tttattcatc acgggttgat
      841 tcgccccacg atggccgaac ttcaacttgt aagtccgagc accacatgcc aaggccagca
      901 gttggtgtcc cagacagatc ccaaagatgg gaactttgcc aaggagctgg gcaatcgtct
      961 tttgggcgta ttggacgggt tccggatcgc cggggccatt ggagaggaat accccatcag
     1021 gccggaagct catgacttcc tcggcagtgg ccgttcccgg aaggaccgtt acccggcaac
     1081 cactgtcgaa gaggtgccgg gcaatgttgt atttcatacc aaaatcgagg gccacgacgt
     1141 ggggcgcatc gggcggaata cgttcgcgga cttgcccacc cagtttcgcc caacgactca
     1201 agggctgtgc ccactcccgc ggaccaggtg gtaaaacctc ccgaaccaag tcgcgtccca
     1261 caaggccggg acttgccttg gccttggcta ccaggctggc gtcatcgaga tcgacggtgg
     1321 atagcacgcc tttcatcgcc ccgtgttccc gaattcggcg aaccaaggcc cgcgtgtcga
     1381 tcccataaag gcctacgatg ccaaatcgtt tgaggaacga atctaaattc tcctgggcgc
     1441 gccgattgct aacgcggcag cttacttcgc gaactacgaa tccggccaag tggggtttcc
     1501 agctttcgaa gtcctcctcg ttgaccccgt aattaccaat ttccgggtag gtcatgacca
     1561 caatttggcc acgatcgctg ggatcggtga ggatctcctg gtagccggtc atcgaggtgt
     1621 tgaagcacac ttcaccgtcg acaactcctt ctgcgccgaa cgagtaaccg cggtaaacgg
     1681 tgccatcttc taaagcaagt ttcgctggtt ttggcatata acgaggtcgt cccgatgctt
     1741 gacttctccg aaagaccaca cctcaaaatg gaaactcgcc taaggcaata aacttctgcc
     1801 gtttcgccca acttttcgtc acttggcggg gtctgcgatg gacctcatgg acgtaaagac
     1861 tctcatctaa agtttgcttc gcaaaaacac cgatacgatc aaacacggct gctgcggtcc
     1921 cgatcacaga cgtcgcatag aagcgtcaac gtcgcgcggc cgacccaaag gttcctgtcc
     1981 cggcgagcag gaaccttttt gttttttgtt tcttggaccg atgctggtcc cgatggccac
     2041 tctcctccaa agccccactg agcgatcggg gcaacttagt gtatcttatg catttttatc
     2101 ttatctgacc accacaccct gggtctg
//
LOCUS       DAOPTR010000746         2124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_40882_length_2124_cov_3.330111, whole genome shotgun sequence.
ACCESSION   DAOPTR010000746 DAOPTR010000000
VERSION     DAOPTR010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40882_length_2124_cov_3.330111"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..>2124
                     /gene="carB"
                     /locus_tag="PKI05_12785"
     CDS             332..>2124
                     /gene="carB"
                     /locus_tag="PKI05_12785"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOA53121.1"
                     /translation="MPRRNDIRKILLIGSGPIVIGQACEFDYSGSQACKALREEGYEV
                     ILVNSNPATIMTDPEMADRTYIEPITWEFVAKIIERERPDALLPTVGGQTGLNVAMDL
                     YRHGILDQFKVELIGANAQAIAKAEERDQFRLAMERIGLDVCRGRTVHNMKEAEEVLK
                     EIGLPCVVRPSFTLGGTGSSVAYNREEFIDLVSRGLTASPIHEVLLEESVLGWKEIEL
                     EVMRDADDNVIIICSIENFDPMGIHTGDSITVAPVQTLTDKEYQRLRDAAIAVIREIG
                     VDTGGSNIQFAIHPHNGRIVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYRLHE
                     LANDITRKTKACFEPTIDYVVTKVPRFAFEKFPEADPTLTTQMKSVGETMAIGRTFKE
                     SLQKALRGLEVGSFGLGCDGKDLWGTPKQPSIDEIRYQLSHPNPQRIWYIRYAFKAGM
                     TVEEVNELTSIDPWFLANIKEIVDFENHLRTYRDISEVSDETLREAKRYGFSDRQLAH
                     IWSRAEMEIREERKRRGIVATFKAVDTCAAEFEAYTPYYYSTYEDEDETPPKKPGKKR
                     IMILGGGPNRIGQGIEFDYCCCHASYALRELG"
BASE COUNT          515 a          555 c          599 g          455 t
ORIGIN      
        1 gctaggtgtt gcggtgcctt tgacaggcat tctcgggttg tggcaaacgc caccaatcgc
       61 agacagggca acgcactacc actcaagggt gggttgcgaa tgaccaaaat ttcggcataa
      121 catgagaaga tttagctaat gcactacgag ccgtgcaaag acacgaatgt caacgacttt
      181 ccgcaaaaaa gtgttgctta acaaatcgtt ggtgatcggg ggttcttcgt ctctcctcgc
      241 cgcctttggt gagaagaggt ccaacgatta gccaagagtg cttcatcgtt gtcccgttca
      301 gagatcgaca gtgtagggag atagtacgga cgtgccacgc cggaacgaca ttcgcaagat
      361 tttgttgatc ggttctggac ccattgtcat cggtcaggct tgtgaatttg actattcggg
      421 ttcgcaggcg tgcaaagcat tgcgtgagga aggctacgag gtcatcctgg tcaattccaa
      481 tccggcgacg atcatgacag acccggaaat ggccgaccgg acctacatcg aaccgatcac
      541 ttgggaattc gttgcgaaga tcatcgaaag ggaacgcccc gacgccctcc tccccacggt
      601 gggaggccag accggcttga atgtggccat ggacctttac cggcacggaa tcctcgatca
      661 atttaaggtc gagttgatcg gggccaatgc ccaggcgatt gctaaggcgg aagaacgcga
      721 ccagttccgc ctggccatgg aaaggatcgg gcttgatgtt tgccgagggc gaacggtcca
      781 caacatgaag gaagccgagg aggtcctcaa agaaatcggc ctgccttgcg ttgttcggcc
      841 gagcttcacc ctgggaggaa ccgggtcaag tgtggcctac aaccgcgagg aattcatcga
      901 ccttgtcagt cgcgggctaa cggcatctcc tattcatgag gttctccttg aggaatcagt
      961 cctggggtgg aaagagatcg aactggaggt catgcgcgat gcggacgaca acgtgatcat
     1021 tatctgttcg atcgagaatt ttgaccccat gggcattcac acgggcgatt cgatcacggt
     1081 cgctcccgtc caaacactga ccgacaagga gtatcaacga ctgcgggacg ccgctattgc
     1141 cgtgattcgc gaaatcggcg tcgatacggg tgggtccaac attcagtttg cgatccatcc
     1201 gcacaacggc cggatcgttg tcatcgagat gaatccccgc gtcagccgct ccagtgcttt
     1261 ggcttcaaaa gccaccggtt ttcccattgc caaaattgcc gccaaattgg ccgtcggcta
     1321 ccgcctccat gaactggcca acgacattac gcgcaagacc aaggcctgct ttgaaccgac
     1381 cattgactac gtcgtcacga aggtcccacg cttcgcgttt gagaagttcc ccgaagcgga
     1441 tccaaccctc accacgcaga tgaagagcgt tggggagacc atggccattg ggcgaacctt
     1501 caaagaatct ttgcaaaagg ccttgcgagg gttggaagtc ggctcatttg gactaggttg
     1561 tgacggaaaa gacctctggg gcacaccaaa gcagcccagt attgacgaga tccgctacca
     1621 actgtcccac cccaatcccc agcgcatctg gtacatccgg tacgccttta aggctggaat
     1681 gaccgtcgag gaagtcaatg agttgacatc catcgatccc tggttcttgg caaacatcaa
     1741 ggaaatcgtt gactttgaaa accatttgcg aacatatcgg gacatcagcg aggtcagtga
     1801 tgagaccttg agggaggcca agcggtacgg attctcggat cgtcaattgg cccatatttg
     1861 gtcgcgggcc gagatggaga ttcgagaaga gagaaaacgc cgggggatcg tggccacatt
     1921 caaggctgtc gatacatgtg cggccgagtt cgaggcttac acgccctatt attattcgac
     1981 ctacgaggat gaagatgaga ccccacccaa gaagccgggc aaaaagcgga tcatgattct
     2041 cgggggtggt cccaatcgaa ttggacaagg tattgagttc gactattgtt gttgtcacgc
     2101 cagttacgct ctccgtgagt tggg
//
LOCUS       DAOPTR010000747         2120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_40995_length_2120_cov_3.015981, whole genome shotgun sequence.
ACCESSION   DAOPTR010000747 DAOPTR010000000
VERSION     DAOPTR010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40995_length_2120_cov_3.015981"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            460..1599
                     /locus_tag="PKI05_12790"
     CDS             460..1599
                     /locus_tag="PKI05_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53122.1"
                     /translation="MRYGAFLCVVLAVLAGCKGPFQQNLPPAQQLMEMGPGVGGPGPG
                     VQAAYMPMLAAAQVPVSQIYFVGPEGMQVTWDVSAPGAFDSEPLICPGRYNFPQGAIY
                     RLKLTNIPGRPGVELYPTLEVAPAMPRTEAFIAHNAIPVQFTEEDFDQVLSGNFVTKV
                     VYLPDPNYQELAVAGAETLVSTRLDPGVDPIIEADRRGSILCVIRVGNRDLQVPGDVA
                     NTADIVPAGAANNGTSLQGTPFAQTPVMAGGGTVPMNYISGVTGPQYGMPISGTPIGL
                     PGPPHVPLGGPAGLQKHVMRNHTFVHVPQPTERVRIDVKQSPGMNYPRPANHAWIHEK
                     TVPGATVFRQPYSAYSQHIPADSEGEGYIVTDPGASSDTASAPAQ"
     gene            1811..>2120
                     /locus_tag="PKI05_12795"
     CDS             1811..>2120
                     /locus_tag="PKI05_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53123.1"
                     /translation="MLPLSDVGSMFVFPFHAGRKGHAAVTLLLFCCFGLAGWPSRVIG
                     QQPPVHPLFDSRTPPGIIGATRLARNGPVDNYYQPVEIRGPHGVQVCLVESGHFGQPR
                     N"
BASE COUNT          446 a          605 c          583 g          486 t
ORIGIN      
        1 gttctctcga tcgactccga cgatggcctg cgctcaccct aaccatctcg cagagggaga
       61 gggaactttg ggataggctc taagcatctc atcctgccca ttttttctaa tcttgctatc
      121 ttccccgtat ggggcatcga gctggttgaa attcgcgccc ggcgcttctt cgcaaccatc
      181 ggcgggattt tgcagattca acgagatatc ccagaggctt ttgaactacg taatgtgaca
      241 catttgccgg atttgccaaa gaatccagcc gcttttttcc gacgaatacc ctcagaggca
      301 ctgcctggag cctgtttttg acggcccgat cgtatcgcgt gagataacgt agcccgcgga
      361 aaaagacggt taagcgaagt gcctcgaagc aacctgaggt agagaatggc gaagcagttt
      421 gggattgccc aagttctggg ttttgagggg gatacctcga tgagatacgg agctttcctc
      481 tgtgtggtgt tggcagtgct ggccggttgt aaaggcccat ttcaacaaaa cctaccccca
      541 gcgcaacaat tgatggaaat ggggccgggt gtgggcggac ctggtcctgg cgttcaagcc
      601 gcgtatatgc ccatgctagc ggcagcccag gtccctgtgt cccagattta ttttgtaggt
      661 cccgaaggga tgcaggtaac ttgggatgtt tcagcccctg gggcgtttga ctcggaaccc
      721 ttgatctgcc caggccggta taacttccct caaggagcta tctaccgcct gaaactcaca
      781 aatatcccag gccgaccggg tgtggagctt tatcccacgc ttgaagtggc gcctgccatg
      841 ccgcggacag aggccttcat cgctcacaat gccattcccg ttcagttcac cgaagaagac
      901 tttgatcaag tcctcagtgg caactttgtg acaaaagttg tgtatctgcc ggatccaaac
      961 taccaagaat tggccgtggc gggtgccgag accttggtca gcactcggct ggaccccggt
     1021 gttgatccga tcatcgaggc cgaccgtcgc ggctcaattc tctgcgttat ccgcgtggga
     1081 aatcgtgatc tccaggtccc gggcgatgtg gcaaatactg cggacatcgt gccagccggt
     1141 gcagctaata acggcacatc acttcaagga acaccctttg cccagacgcc ggtcatggcg
     1201 ggtggcggta ccgttccaat gaattacatc tcgggcgtga ccggtcctca atacggcatg
     1261 ccgatcagtg gtacccccat tggtctgccc ggcccaccgc atgtcccatt gggaggtccc
     1321 gccggtctcc aaaagcacgt catgcggaat cacacgtttg tgcacgtgcc acagcctaca
     1381 gagcgagttc gcatcgacgt gaagcaatcc ccaggcatga attacccgcg acctgctaat
     1441 cacgcctgga ttcacgagaa aaccgtgccc ggtgccacag tgttccgtca accatattca
     1501 gcgtatagcc aacacattcc tgcggattcc gaaggcgaag gctacattgt gacagacccc
     1561 ggcgcatctt ccgatacggc ctcagccccg gcacagtgat gttggaggcc ccgtggtttt
     1621 gatgaccttt tgggggtatt ccacagggct gtcggcccga gatccactct caacgccgac
     1681 agcccttttt tgcagggaaa gcgtcgaaca cagaggacaa ggcggtttcg ctcaagattg
     1741 cgttaaggtg tgacgattgt actgattcta agcggttacg gacttcaaaa gacatctcga
     1801 ggtgcaggcc gtgttgccat tgtctgacgt gggttccatg ttcgttttcc ccttccatgc
     1861 aggccgtaag ggacacgccg cggtaacact tttgctcttc tgttgtttcg ggttagcagg
     1921 gtggcccagt cgcgtcatcg gccaacagcc accggtccat cctttgttcg actcgcggac
     1981 gccaccggga atcattgggg caacacgatt ggcccgtaac ggcccggttg ataactacta
     2041 ccaacccgta gaaatccgcg gaccacacgg cgttcaggtc tgtctggtcg agtcgggaca
     2101 ttttggtcaa ccgcggaacc
//
LOCUS       DAOPTR010000748         2117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_41059_length_2117_cov_3.480601, whole genome shotgun sequence.
ACCESSION   DAOPTR010000748 DAOPTR010000000
VERSION     DAOPTR010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41059_length_2117_cov_3.480601"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2117
                     /locus_tag="PKI05_12800"
     CDS             <1..>2117
                     /locus_tag="PKI05_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53124.1"
                     /translation="VHVQGKCFEDYAELSVRVRIRLRGDQPYKIPLRLEQLIPRELPR
                     RVNGQPLLFSYSRQDGGYTLYLQGTADSVEEIIFQGWAPVSHVGSEARLVLSVPEAPI
                     SDLELDVPLADIEPSVTPGAMILGMDAVEGGRRLRVTGLGPGFVLTWTIPPSAAWDTN
                     TVLESNGAISVRVTERMVDFDARLNVRSYGIPFDRFRVTLPIGARPLPFSSTSYTVQE
                     VKREDQAGASQSDRTTVEVLLAKKTTGPVEVRLTAQCEKEPHAWIDIGGFSVEGAVRQ
                     SGYLSVMPSSDQALIWGEWKGVRQVSHIPTAANGEASETVVHLEYFMQPCTVVLKSVP
                     RVAYVSIAPRYRVELTTEQALLQARLNYDVQGADVIALDIDLAGWQLQEALLGEQSTV
                     LAVTGDPKGRATLALPQPMSGKLEITLLATRDRPLENKPLMLSFPQPKAQVVQPAEVL
                     IMAPANLELRADYKESEGVEQALPQATEKGVSAVNVVSFRLNRPDARFVADWTTHEQD
                     VSVDIVTRVRPEGAQLTVEQQIRYQVRYEPWTGAWRFRVPSGIEQITVLLDGRPVTLT
                     PVAAETPTQSAAESEKPSSGVILDSAGNVFAVLPIGPILGTTELVFRYIVPGGLASPE
                     ATRWQIPLVDPLDGRLQQHRLSISASVGWNIESVVAPWQIRETTSLSAVSASQIECET
                     KEHTTQIVLRPRVGTFGETNVVV"
BASE COUNT          441 a          576 c          630 g          470 t
ORIGIN      
        1 aggttcacgt tcaagggaaa tgctttgaag attatgccga gttatcggtt cgagtgcgga
       61 ttcgtctccg cggggatcag ccgtacaaaa tccccctaag attggagcaa ctcatccctc
      121 gggagcttcc tcggcgggtg aatggccaac ccttgctatt ctcgtacagc cgtcaggacg
      181 gaggttacac tttgtatctt caagggacgg cggattcggt ggaggagatc atattccagg
      241 gatgggcacc cgtctctcat gtggggagcg aggcgcgact cgttctcagt gtgcccgaag
      301 cgcctatctc tgatttggaa ctcgatgttc cgctcgctga cattgagccc agcgttaccc
      361 caggggcgat gatcttgggc atggatgcgg tggaaggtgg tcgccgccta agagtgacgg
      421 ggcttgggcc gggctttgtc ctgacctgga ctattcctcc cagtgctgcg tgggatacca
      481 acacggtgtt agagagcaac ggggctattt ctgtgcgagt gacggagcgc atggtggatt
      541 tcgatgcacg cctcaacgtg cggagttatg gaataccgtt cgaccgtttt cgcgtaacgc
      601 tgccgattgg tgcccgacct ctaccttttt cttcgacgag ttacacggtc caagaggtta
      661 aacgagaaga tcaggcggga gcgtcgcaat cagatcgcac cacggttgag gtcctcctgg
      721 ccaagaaaac gactggcccg gtcgaggtcc gcttaaccgc ccaatgcgag aaggaacccc
      781 acgcgtggat cgacattgga gggttctccg tggaaggcgc cgtccgccag tccggctatt
      841 tgtccgtcat gccttccagt gaccaagccc tgatctgggg cgagtggaag ggggttcggc
      901 aggtttctca cattccgacc gcagcaaatg gagaggcctc cgagaccgtc gtacaccttg
      961 agtattttat gcagccgtgc acagttgtcc tgaagagtgt ccctcgggtg gcctatgtga
     1021 gcattgcccc gcggtatcgt gtggaactga cgaccgaaca ggcactgctt caggcaaggc
     1081 tgaactatga tgtccagggg gcagacgtta tcgcgctgga catcgacctt gcaggctggc
     1141 agttgcagga agcccttttg ggcgagcaga gcaccgtatt ggccgtgacg ggtgatccca
     1201 aaggacgcgc gaccctggct ttgccgcagc ctatgtctgg taaactcgaa atcaccctcc
     1261 tcgctactcg agacagacct ttggaaaaca agccgcttat gctgtctttt ccgcaaccca
     1321 aggcccaggt cgttcagccc gccgaagtcc tcatcatggc cccggccaat ctggagttgc
     1381 gggcagatta caaggaatcg gaaggagtgg aacaggcgct tcctcaggca actgaaaagg
     1441 gagtgtccgc cgtcaatgtt gtctcattcc gcctcaatag accggacgct cgcttcgtcg
     1501 ccgattggac gactcatgag caagacgtct ccgtggacat cgtcacccga gtcaggccag
     1561 aaggcgctca attgactgtt gagcaacaga tacgctatca ggtccgttac gaaccctgga
     1621 caggtgcttg gcggtttcgc gtcccctcag ggatcgagca gataactgtt cttttggacg
     1681 gtcggccggt cactttaacg ccggtcgcag cggaaacccc gacacaatcg gcggcggaat
     1741 cagagaagcc atcttcaggc gttattttag actccgctgg caacgttttc gcggtgttac
     1801 cgattggacc tattcttggc acaacggagt tggtatttcg ctacatagtc cctggtggat
     1861 tggcgtcccc cgaagcaaca cgctggcaaa ttcccctggt cgatccgctg gacgggcggc
     1921 ttcaacagca tcgattgtca atttcggctt ccgtcggttg gaacatcgaa agtgtggtcg
     1981 caccgtggca aattcgcgag acaacgtcgt taagtgcggt ttctgcctcg caaatcgagt
     2041 gtgaaaccaa agagcatacg acacagattg tcctacgtcc tcgggtaggg acattcgggg
     2101 agacaaacgt ggtggtc
//
LOCUS       DAOPTR010000749         2115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_41119_length_2115_cov_2.511650, whole genome shotgun sequence.
ACCESSION   DAOPTR010000749 DAOPTR010000000
VERSION     DAOPTR010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41119_length_2115_cov_2.511650"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="PKI05_12805"
     CDS             complement(<1..764)
                     /locus_tag="PKI05_12805"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA53125.1"
                     /translation="MRVIRQLMFWVACGFAWGVVTQAVFARSGAGERPNILVLIADDQ
                     RYDALSVVQKEQGERGRFPWFQTPHLDRLAAEGVRFRNAFVVNSLCAPSRAVMLTGRY
                     NHLNGIASNFRPFPLETVTYATILKQAGYVTGYFGKWHMDSQRERPGFDFFASFIGQG
                     VYFNCPLLVQGVETRTEGWVDDVATDYALKFIRETTAEKKPWLVVLGFKSPHGPFTPP
                     ERAKERFTGCLARRTPNFDVRPPYLPEARTQQTSGEA"
     gene            complement(1233..1451)
                     /locus_tag="PKI05_12810"
     CDS             complement(1233..1451)
                     /locus_tag="PKI05_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53126.1"
                     /translation="MPLPPGDLAAFPPERNLLPTATHPVFAGDGRMLLVPEDARREVK
                     PVGGEDRIDEVSPPVNTTLVVGIGWLPL"
     gene            complement(1661..>2115)
                     /locus_tag="PKI05_12815"
     CDS             complement(1661..>2115)
                     /locus_tag="PKI05_12815"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOA53127.1"
                     /translation="LELEGSNLSDREFEDRIASTLTALGFKVTQKGHKKEGAYADGVA
                     IHEDTGIVYDCKNSQNYAPTEDDIRALKQYTSEEMTLNSGKALNGAFIARDFRTQPRQ
                     NIFHLPVEALLYLLFVKLSKGNEFNLNPFKLLLKKGGELSRRKIDEYW"
BASE COUNT          515 a          618 c          524 g          458 t
ORIGIN      
        1 gcctcaccgc tggtctgctg ggttcttgct tccggaaggt agggaggccg gacatcgaaa
       61 ttcggtgttc gtctggccaa acagccggta aaccgctcct ttgcccgttc cggtggcgtg
      121 aacggaccat gaggggattt aaacccgaga acgacgagcc acggtttttt ctcggccgtt
      181 gtttcacgga tgaatttgag ggcataatcg gtggccacgt catcaaccca gccctcggtc
      241 cgcgtttcca caccctgtac gagcagtggg caattgaagt acacgccctg accgataaaa
      301 ctggcaaaga aatcaaaacc gggacgctct cgttggctat ccatgtgcca tttgccaaaa
      361 taacccgtga cataccccgc ttgcttgagg atcgtggcgt aggtcacggt ctccaaggga
      421 aatggccgaa agttagaggc aatcccgttc aggtggttgt accgacctgt caacatcacc
      481 gcccgagagg gcgcacaaag cgaattcaca acaaaggcat tgcgaaaacg gacgccttct
      541 gccgctagac gatcgaggtg aggtgtttga aaccaaggaa atcgcccacg ttctccctgc
      601 tccttctgga ccacacttag cgcatcgtag cgctggtcat ccgcaatcag aacaaggatg
      661 ttgggacgct ccccagctcc acttcgagca aaaaccgcct gggtcacaac cccccaggca
      721 aatccgcacg caacccaaaa catcaactgc cgaatgacgc gcatcgccta tgtcctccct
      781 caagaaagtg ccttcgcctc tcaagcaaga cccggtgctt tcacttctaa ttgcccgaat
      841 aatgccaaaa gcacctccta gtctaacctg aaaacattcg ggacctagac tagtaccacc
      901 aacatggcgt gtatatccca tctgcagtgc tatccagcaa ggctcagagg agtcacgcgc
      961 cttcggtacg aggggacatg ttctgacgac cttggtcttt ttcagcaagt aggcccaaag
     1021 tcgtcacaag acctacaacc cctcgtcgat agaaggccgc gacgaggcca aacgctggca
     1081 accgctttca aaaatgacgc ggacttggtg gatgccaaaa ccagccgcaa agagtagtaa
     1141 cgaattagaa ctaccagtgg ccgtaaggct agcacccgct gggaggcagc atggtcaagc
     1201 ggttgtgaga gaacgtggga acgacacacc gctcacagcg gcagccagcc gatcccgaca
     1261 acaagcgtgg tattcaccgg tggtgagact tcatcgatcc ggtcctcccc gcccacaggc
     1321 tttacttctc gccgggcatc ctctgggaca aggagcatcc ggccatcacc cgcaaagacg
     1381 ggatgagtag ccgtggggag taggtttcgc tcgggtggaa acgcggcaag gtcgccgggc
     1441 ggtaacggca cgcgcttcac ctgcgccgcg aagcgcagcc gagcggcgtc acgtgcaagc
     1501 gcgggttatg ccacatttcg ccttaaaagc cacaccggct aacgcagtta acgccatagc
     1561 accacccaac ggcaaccgaa tcaaaaccca ttgccgtttg caggccgccc cgccgttgcc
     1621 gtactgcgtg tcttctccgt ttgtggattc gcgcttatgg ctaccaatac tcatcaattt
     1681 tccggcgact caattcgcct ccctttttaa gaagcaactt aaatggattc aaattaaatt
     1741 cgtttccctt gctgagtttc acaaaaagca aataaagcag tgcctccact ggcaaatgaa
     1801 aaatgttctg acgaggttgc gtccgaaaat ctctagcgat aaacgcgcca ttgagggcct
     1861 tgcccgagtt caaagtcatt tcctcactag tgtattgttt cagggcgcga atatcatctt
     1921 cggttggcgc atagttctgg ctattcttgc agtcgtacac tatgcctgta tcttcgtgaa
     1981 tggcaacacc gtctgcataa gcaccttctt ttttatgccc tttttgagtt actttgaatc
     2041 ctaacgccgt cagcgtggaa gcgattcggt cttcaaattc cctgtcgctg agattactcc
     2101 cttccaattc taaaa
//
LOCUS       DAOPTR010000750         2112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_41191_length_2112_cov_4.305785, whole genome shotgun sequence.
ACCESSION   DAOPTR010000750 DAOPTR010000000
VERSION     DAOPTR010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41191_length_2112_cov_4.305785"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PKI05_12820"
     CDS             complement(<1..830)
                     /locus_tag="PKI05_12820"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HOA53128.1"
                     /translation="MRTSVPLEQLGRLLEGQHENPFEVLGPHVVEDGDRRVLAIRAFL
                     PHSQQAWVIDLAHEGVPRPMRRIHPAGLYEAVCPLNGQRPEYLLKVADQDGKQVIMHD
                     PYAFPPLLTEYDLYLLNEGRHWRSYNKLGAQFRVVNGVEGVNFAVWAPNATSVSVIGD
                     FNRWDGRCHPMRKHIPSGFWELFIPGLKEGDLYKFQIRFHDQVFEKADPFAFYAEVPP
                     RSASRVFNINRYQWQDREWMEQRPKRNWLEEPIAIYEVHLGSWRRSPNGHNGWMNYRE
                     L"
     gene            complement(1151..1789)
                     /locus_tag="PKI05_12825"
     CDS             complement(1151..1789)
                     /locus_tag="PKI05_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53129.1"
                     /translation="MGNSVSRWDKSAFGLMDPAAPDSGGRLTFTLLYEVGIEGNPHRW
                     RFILRSPDGHDELIAEDVEPGVQGERLELLTVLRALESLNQSSRIIIWTRSPALKEGL
                     LYGLRDWPLHDWCWERFGERVPVRNRDLWQRLERVIQFHEIECRAWRFDPPHVHEVSP
                     PNSRGFGQRAFAPGKPKSGCQNGQGDSPGGSSWKWLFGAFLPRRSTVSNLPK"
BASE COUNT          490 a          651 c          518 g          453 t
ORIGIN      
        1 agctcacgat aattcatcca accgttgtgg ccattaggcg agcgccgcca actccccagg
       61 tggacctcat aaatcgcgat gggttcttcc aaccagttcc gcttgggccg ctgttccatc
      121 cactctcggt cttgccactg ataccggttg atattgaaaa cgcgtgaggc actacgtggc
      181 ggaacctcgg cgtaaaaagc aaaaggatcg gccttctcga acacctgatc gtggaagcgg
      241 atctggaact tatagagatc gccttctttc aagccgggaa taaacagttc ccaaaagccg
      301 ctgggaatgt gtttccgcat ggggtggcaa cgaccgtccc agcgattgaa gtctccgatg
      361 acactgacac ttgtcgcatt gggtgcccaa accgcaaaat tgacgccctc caccccattg
      421 accacacgaa actgagcgcc aagcttgtta taagaccgcc agtgccgccc ttcattgagc
      481 agatacaggt catactcggt cagcaaagga ggaaacgcat acggatcgtg cataatgacc
      541 tgcttgccgt cttgatcagc gactttcaat aaatactcgg gccgttggcc gttgagggga
      601 catacggcct cataaagtcc cgccgggtga atccgtcgca tcggccgcgg aacaccctca
      661 tgcgccagat caatgaccca ggcctgctgc gaatggggca aaaacgcccg aatcgccaag
      721 acccgccggt caccatcttc caccacatga ggacccagaa cttcaaaggg attctcgtgt
      781 tgaccctcca acagtcggcc aagttgctct aacggaacgc tcgtccgcac agaaacctcc
      841 ttgtcaccca gatgattcta ccctaatagt ttacccaaac ctcaaggatc tctcatgctc
      901 ccaataaaca aggacgcccc tttttgtcta atcgtctgca tcgcagctat tttcaatagt
      961 ttgccccagt tgtccgcatt atcagtcgct tccgcatcag tcgttccaaa cgatcgatcc
     1021 gaccttatgt cccagccgca gtctatcccg gatgcttttt ttcgagccta tcccataatc
     1081 gctcctctcc cggggggaga ggggggactt ttgcgatagg ctcttagtcg tttccgcggg
     1141 gagcccaggc ctacttgggt aaattgctca ctgtggaacg acgcggaaga aatgccccga
     1201 aaagccattt ccaggaactt ccacccggcg agtcaccttg cccattttga cagccgcttt
     1261 ttggctttcc cggcgcaaaa gctcgttggc caaagccgcg ggaattcggt gggctgacct
     1321 cgtgaacatg tgggggatca aaccgccacg cccggcattc gatctcatga aattgtatga
     1381 cacgctccag ccgttgccac aaatcccggt tcctgaccgg cacccgctct ccaaatcgtt
     1441 cccaacacca atcatgcaac ggccaatcgc gaaggccgta gagcaacccc tctttcagtg
     1501 ctgggctccg tgtccaaata atgatccgcg aggattggtt cagcgattcc aatgcccgca
     1561 ggaccgtcag cagctcaagc cgctcaccct ggacacccgg ttccacgtcc tcggcgatta
     1621 attcatcgtg cccatcgggg gagcggagga taaaccgcca gcggtggggg ttaccctcaa
     1681 ttccgacttc gtacaaaaga gtaaacgtga gtcgtccgcc gctatcgggc gcggccggat
     1741 ccatcagacc aaacgccgat ttgtcccatc gagaaacact attacccaca gtggtgcctc
     1801 accttgagac gttgatgccc aatggaatgt gatgacgata gccctcaaga gtggggaggt
     1861 cgcagcgtta ccggaaccca ttccaaaaac cacccgacga cggtgccgca acccctcttt
     1921 gccttgtatc gtcacagagc ccctaaagtg tgcagtttat ccaacaagaa aactttaaca
     1981 ggaggcggca agtaacctgc ctgtgttggt ccccgcggtg ccagtcttag ccctgcttct
     2041 gaagattgtg ccaccagtcg cagccgtaat tgccaacctt gtactgctca atctggccgg
     2101 acgcggctgg gc
//
LOCUS       DAOPTR010000751         2112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_41194_length_2112_cov_3.778318, whole genome shotgun sequence.
ACCESSION   DAOPTR010000751 DAOPTR010000000
VERSION     DAOPTR010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41194_length_2112_cov_3.778318"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..582)
                     /locus_tag="PKI05_12830"
     CDS             complement(220..582)
                     /locus_tag="PKI05_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53130.1"
                     /translation="MQKVLVQLWSQEDGVLTFEWILLITILVIGVVGGLSAVRDAVIT
                     ELGDVVEAVISLDQSYLLLDPWEIRVPDCEADGASGSSYQDSAGMSQERLGNCNQGCF
                     LEQDQDLGDCSPYIERPF"
     gene            1163..>2112
                     /locus_tag="PKI05_12835"
     CDS             1163..>2112
                     /locus_tag="PKI05_12835"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HOA53131.1"
                     /translation="MNKLTNLLLEKEILLESGTNELEILVFDVGQYVFGVNVAKVREV
                     LPAPTITHLPNAHPSIRGVFKLRNQVVPCVSLADYLGQYDGGDDRESVCILTDLNQQQ
                     TAFLVDRVERIHRLSWENVLAVPPLEDLAQTPVTALARCQDRLILMLDFEMILDQVTE
                     HYFRTESVENPASLPREQAVILLVEDSPTVREAITQTLSASGYSRLVVFENGKHAWEW
                     LEKKVNDQSSHGCLCDLVIADIEMPQVDGLHLTKRIKSHPQLKHIPVLLYSSIITPEN
                     RRKGEAVGADAQVAKPDLCHVVEYADNLIFGLRDNSGSHF"
BASE COUNT          515 a          575 c          558 g          464 t
ORIGIN      
        1 tgtgccgggc gatggcccat agagcatggt cgagagcaca aacaacggga gccagcagac
       61 gtgaatcatc tctgacgctt ctcctcacaa gtgggggatg taacagtcgt tacattcagc
      121 ataatcagaa tcagccgatc caacaaacaa tgaccgccgt acctgtttag gtacaacggt
      181 cgcggattct ttttaggcaa gtgagccccg atgacttact cagaacggcc gctcgatata
      241 cgggctgcag tcccccaggt cctggtcttg ctccaggaaa cacccctggt tacagtttcc
      301 gagccgctcc tgcgacattc cggcggaatc ctggtaagaa gagccgctgg caccatcggc
      361 ttcgcaatcc gggacacgaa tttcccatgg gtcgagaagg agataggact ggtccagaga
      421 aatgacggcc tcaaccacat cacccagctc agtgatgacg gcatcccgga cggcgctcag
      481 tcctcccaca actccgataa cgagaatcgt gatgagcaga atccattcga aggtgaggac
      541 accatcttcc tgggaccata attgcacgag gactttctgc acggagattt cctcccttta
      601 ctagcctgcc tgtgatttgc tcccgctcgc tcatttacga tataccacgc cttaggtttg
      661 agatcgctgt gcaaatggga tgccgcacgg acaattcccg gcaggtaaat ttcacaatgc
      721 ggcataacta actctcggta aggcacttac ggcgtaaatc ctcgcccggc cggaaggttg
      781 gcagaaaggc ccgcatccgc gaacatgtag tccggcaaaa ggtttgtttc aaaatcacaa
      841 cgttttgtat cggacatagg tgccgcggtt tacagcttga aacgaggtca gccacgacat
      901 gaccgtaatc accccgcggt ttcaatagtt gtaacgatcg tagcggatgc aaaaaggaac
      961 aacgagcgcg tatgacgccc cttctggaat gatcaggcgc cgccgtggaa ttcagaacct
     1021 aagtttgaga gaacccacct aataactgcc ccccaagcga ggcgaatctg tttttcattc
     1081 ccacccaggc gggggttact ctacgggcgt ttgcaccgtg ctttgtgtgg tcaagtagtg
     1141 ccagcaaaaa ggaagacgag ttatgaataa gctcaccaac ctgcttctgg aaaaggaaat
     1201 cctgctggag agcggtacga acgaactcga aatcctcgta ttcgacgttg gtcaatacgt
     1261 ctttggcgtt aatgtggcaa aagttcgcga ggtactgccc gcacctacca tcacccattt
     1321 gcccaacgcc caccccagca ttcggggagt cttcaagctg agaaatcaag tcgttccgtg
     1381 tgtctccttg gcggattact tgggtcaata tgacggtggt gacgatcggg aatcggtatg
     1441 cattcttaca gacctgaacc agcagcaaac ggcgtttctt gtagatcgtg tggagaggat
     1501 tcaccggctg agctgggaaa acgtgctggc tgtaccgccg cttgaggatc tcgctcagac
     1561 gcctgtcacg gcactagccc gctgccaaga ccgcctcatc cttatgctcg actttgaaat
     1621 gatccttgac caggttaccg aacactactt ccggacagaa tcagtggaaa atccggcctc
     1681 gctgccccgc gagcaagccg tcattttgtt ggtggaggac tcgccgaccg tgcgcgaggc
     1741 catcacgcaa acactttctg ccagtggata ttcccggctg gttgtatttg agaacggaaa
     1801 gcacgcctgg gagtggctgg aaaagaaggt caatgaccag tcatcgcacg gttgcctttg
     1861 cgatctcgtc attgccgata tcgaaatgcc ccaggtggat ggtttgcatc tgacaaagag
     1921 gattaagagc catccgcagc taaagcacat accggtcctg ctgtactcct ccattattac
     1981 cccggaaaac aggcggaagg gtgaggctgt gggtgccgat gctcaggtcg ccaaaccgga
     2041 cttgtgtcat gtggtggaat acgccgacaa ccttatcttt ggcctgcggg acaatagcgg
     2101 gtcacatttt ag
//
LOCUS       DAOPTR010000752         2112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_41197_length_2112_cov_3.415168, whole genome shotgun sequence.
ACCESSION   DAOPTR010000752 DAOPTR010000000
VERSION     DAOPTR010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41197_length_2112_cov_3.415168"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /gene="thiE"
                     /locus_tag="PKI05_12840"
     CDS             <1..841
                     /gene="thiE"
                     /locus_tag="PKI05_12840"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOA53132.1"
                     /translation="GTEIKATTELHRMAVEDLVDANFSRVEEALRSIEECAKILVPQV
                     AFGIEQLRYRCYTVHRALRISETAAQRLQNVRLYVLVDARSSAEELRNLVASLIDAGV
                     HAIQLREKHLCDRLLLERARLIREMTRGTNTLFIMNDRADLAVLSEADGVHVGQEELT
                     VKDARFIVGPRALVGVSTHNIEQARQAVLDGADYIGCGPTFPSTTKSFSEFAGLAFLR
                     QVAQEIRLPAFAIGGINEENLASVLATGIQRVAVSGCVIQAANPAEVARRLLARLERP
                     SVD"
     gene            complement(906..2036)
                     /locus_tag="PKI05_12845"
     CDS             complement(906..2036)
                     /locus_tag="PKI05_12845"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA53133.1"
                     /translation="MSGGPSVASVQQSIGGDKPSQSFGAEVWQRLQRDRVARISLWIL
                     GTISVLALITPLLPLMPPRLVRTERQFQPPQFWPLFQHGFYDRPRWAASHSAEDEKQA
                     AEHLLSEGFGHLDPFSQALLRIRWMVFGEWSLAPICGTDKLGRDVFSRIMWGARVSLA
                     VGLVATLVSLLIGVTYGAIAGFVGGFLDRFMMRIVDILYSVPFIFLVIFIITIVSAEP
                     IRNFLETRWGLSRIIIFFFLVGAIYWLTMARVVRGQVISLKHELYVEAARALGASFWR
                     ILFRHIVPNLISIIVVYLTLTIPRVMLFEAFLSFLGLGVEPPDVSWGLLANEGLQAMT
                     PVRIYWWLIVFPGLALAATLFALNFLGDSLRDAIDPRLRNVR"
BASE COUNT          513 a          580 c          606 g          413 t
ORIGIN      
        1 ggggacggag atcaaagcga ccaccgagct tcaccgcatg gcggtggagg accttgtcga
       61 tgctaatttc tcgcgggtgg aagaggcatt gcggagcatt gaggagtgtg ccaagatttt
      121 ggttcctcag gtagctttcg gtatcgagca attgcggtat cggtgttata cagtccatcg
      181 ggcgcttcgt atcagtgaaa ctgccgcaca acgtttgcag aatgtgcgcc tttatgtgct
      241 ggtggatgcc aggtcctcgg cggaagagct gcgaaacctg gtggcttcac tcatcgatgc
      301 cggggtccac gccatccaac tccgtgagaa acatctctgc gatcgcctgc tgctggaacg
      361 ggcccgcctc attcgcgaga tgacccgtgg gaccaacacg ctgtttatca tgaatgatcg
      421 agcagacttg gccgtcttaa gtgaggctga cggggttcat gtggggcagg aagaactaac
      481 agttaaagat gcccgtttca tcgtgggccc ccgtgccctt gtgggagttt ccacccataa
      541 catcgaacag gcccgacaag ccgttttgga tggggccgac tatattggct gcggtccgac
      601 attcccctcc accacgaaga gtttctcgga gtttgccggt ctggcttttc ttcgccaggt
      661 tgcccaagag attcgtctcc cggcgtttgc catcggtggg atcaacgagg aaaacctcgc
      721 gtccgtattg gccacgggaa ttcaacgtgt ggccgtgagt ggttgcgtaa ttcaagcggc
      781 taaccctgcc gaggtggctc ggaggctctt ggcccgtctg gaacgccctt ctgtggattg
      841 agacgtcggt taccgcggcc ttcctgtttg ctggcttcac cagaaagaga tcgtgacgag
      901 cgtcatcacc tgacgtttct caatcttgga tcaatggcat cccgaaggct atcaccaagg
      961 aagttcaagg caaacagcgt agccgccaaa gccaggccgg gaaagacaat caaccaccag
     1021 tagatgcgga caggtgtcat ggcctgaagt ccctcgttag ccagcagtcc ccaagagaca
     1081 tccggtggtt ccacccctaa gcccaaaaac gatagaaatg cctcgaaaag catcacccgc
     1141 gggatggtca gcgtcaaata gaccacgatg atgcttatca aattggggac gatgtggcga
     1201 aaaagaatcc gccaaaaact ggccccgagg gcccgggcgg cttcaacgta cagctcatgc
     1261 ttgagcgaaa tcacctggcc gcgaacaacc cgggccatcg tcaaccagta gatggccccg
     1321 accagaaaaa agaaaatgat gatacggctg agtccccagc gagtttccag aaaattgcgg
     1381 atcggctcag cactgacaat cgtgatgatg aagatgacca aaaaaataaa tgggacagaa
     1441 tagaggatat ccacaatacg catcatgaag cggtccaaga aaccgcccac aaaaccggca
     1501 atcgcgccat aggtcacgcc gataaggagc gagaccaagg tagccacaag gcccaccgct
     1561 aacgatacgc gtgcccccca cataatccgg gagaaaacgt ctcgccccag cttgtcggtc
     1621 ccgcaaatgg gggccaggga ccattcccca aagaccatcc agcggatacg aaggagggct
     1681 tgactgaacg ggtctagatg cccaaagcct tccgacagca agtgctcggc agcctgtttt
     1741 tcatcttctg cggagtgtga tgccgcccat cggggtctgt cgtaaaatcc atgctggaac
     1801 agcggccaga actgtggcgg ttggaattgc cgctcggtcc ggaccaggcg aggtggcatg
     1861 agtggcaaca atggcgttat cagggccaag acggaaatgg tcccaaggat ccaaagggaa
     1921 atgcgggcaa cacgatcccg ctgcaaacgt tgccacacct cggcgccaaa ggattgcgac
     1981 ggcttatccc cgccgatgga ttgttggacg gacgctacag acggtccacc actcatcgct
     2041 aggagaggat gctatctcaa ggcaagggtg gaagttcaat gccggctgcc tttggcggat
     2101 cactctgatc cg
//
LOCUS       DAOPTR010000753         2108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_41297_length_2108_cov_4.321968, whole genome shotgun sequence.
ACCESSION   DAOPTR010000753 DAOPTR010000000
VERSION     DAOPTR010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41297_length_2108_cov_4.321968"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="PKI05_12850"
     CDS             <1..1330
                     /locus_tag="PKI05_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53134.1"
                     /translation="LIRGDPGCVAVTWIDLSEVSSLRSYLIVPKRLEWPSDLVNIYGL
                     RPVPQEKLKGINIKADEIAYELTQTTILPHIVGKPSPAVHWEDAEIRIQVSPEGHILG
                     LLTVWFTSSPAEGYQIQMPKGIVPVNAWLDHRCVPVYQRPDGTTHIATDTVKDHRLDL
                     LFAGSVARRGQSLSLAVPQLKNAEKAESGRVYLLLQAVSSWNPSVAPGEPVTAWDYHL
                     TRAREAAERLLQLSEGAAGPAPPQGTFGELLKQYLLARDEARRALLLAPQTAAISYAE
                     VRLQNLELQVSDLLQSAVGIEATLASPGAIAGETLGDALPSSDMQSWYFECEDLPTQI
                     RLQQTVSAQKWGDLLIGSVVIFAVGSIGWLIRRINRPLSVWMMRFAHPLAVLFGIGWW
                     LWLRPSFVGWLLILAALILLIRSRWQWVPKEDAIVPLIVQQKLPTTGSPR"
     gene            1688..>2108
                     /locus_tag="PKI05_12855"
     CDS             1688..>2108
                     /locus_tag="PKI05_12855"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HOA53135.1"
                     /translation="MSRKVYIFQNARGEPPGQIVDYLDWVGVRFELIPLFHIRESVSP
                     LDWREVCGLVVLGGPMNVDEVEAYPFLMWERLQLKEAVRREIPVLGICLGAQLLARAL
                     GAKVYPNPVREIGWYRVTLTEGCQSDPLFAGASSPATV"
BASE COUNT          424 a          594 c          591 g          499 t
ORIGIN      
        1 actgatacgt ggcgacccgg gttgtgttgc tgtgacttgg attgatctct cagaagtttc
       61 ctccctccga tcgtacctta ttgtgccgaa acgtctggag tggccaagcg atcttgtgaa
      121 catctacggg ttgcgacccg tccctcaaga aaagttaaaa ggaatcaaca tcaaggcaga
      181 cgagatcgca tacgaattga cccaaacaac aatccttccg cacatcgtag gcaaaccctc
      241 ccctgccgtc cactgggaag atgcggaaat acggattcaa gtctctcccg agggacacat
      301 cttgggcctg cttactgtgt ggttcacaag ttcgcctgcg gagggctatc aaatccaaat
      361 gccaaagggg atcgttccgg ttaatgcttg gctcgaccac cggtgcgtgc ccgtttacca
      421 gcgccccgat ggcacaaccc acattgccac agatacggtc aaagatcacc ggttggacct
      481 cctgttcgcc ggcagcgtcg ctcggagagg tcagagcctt agcctggcag ttccgcaact
      541 gaagaatgcg gaaaaggcag aatccgggcg agtctatctt ctgttgcagg ccgtctccag
      601 ttggaatccg tctgtcgccc ctggtgagcc ggtcactgcg tgggactatc acttgacccg
      661 cgcgcgagag gccgccgagc gtttgctcca actctcagaa ggagcggcag gtcctgcgcc
      721 acctcagggt acctttgggg aactgctaaa gcagtacctc ttggcacgag acgaagcacg
      781 acgtgccctt ctgctggcac ctcaaaccgc tgctatcagt tacgcggaag taaggcttca
      841 aaatcttgaa ttgcaagtga gcgatttgtt gcaatctgcc gtggggattg aagccacact
      901 ggcgtcccct ggggccatcg caggcgaaac cctgggtgac gcgctaccat cgtcggatat
      961 gcaatcctgg tactttgagt gtgaggacct tcccacacaa atccgccttc aacaaaccgt
     1021 tagcgcccaa aaatggggag atttgcttat cggcagcgtt gtgatcttcg cggtaggtag
     1081 cataggctgg ctgatccgtc gcatcaatcg gccactttcc gtatggatga tgcgatttgc
     1141 ccatcccttg gccgttctct tcgggatcgg ttggtggttg tggcttcgtc cgagctttgt
     1201 cggctggctt ctcatccttg ctgcccttat tctgcttatc cgatctcgct ggcaatgggt
     1261 gcctaaagaa gatgcaatcg tgcccttaat tgtgcaacag aagttgccaa caacgggcag
     1321 tccgcgataa ttccacgtgg tgtgtgacac gttgcctccc ttacttccac ttcttcgctt
     1381 gccaactggc cgtgtcatgc ttttcggcac ggcacttgct gtgggacttg tttatagcct
     1441 atcccaaagc tccctctctc cccctgggag agggttgggg tgagggcaga cgattttccg
     1501 agtcgatcga gaaagccgac agtgatttga gccccctcac ccccggcccc tctcccgctg
     1561 aacgggcgag gggaggttgt gggataggct cttagtccac agtggctgtc ccatggatgg
     1621 gtccatttag tgaaagcggc ttgacctagt tgtgtgccga gtccgacttg gcgatggatg
     1681 aggtgttatg tcgcgtaaag tctatatctt ccaaaacgcc cgtggcgaac cccccgggca
     1741 gatcgtcgat tatctcgact gggtcggcgt acgtttcgaa ctgatcccac tgttccatat
     1801 ccgcgagagt gtgtcccctc tggattggcg tgaggtatgc gggctggttg tcctcggcgg
     1861 cccaatgaac gtggatgagg tagaggcata cccttttctg atgtgggaac gacttcaact
     1921 gaaagaggca gtacgccgcg agattcccgt cctcggaatc tgcttggggg cccaattgct
     1981 tgcccgcgca cttggggcca aagtctatcc caatccggtt cgcgaaatcg gctggtacag
     2041 agtaacgttg acggaagggt gccaaagtga cccgctgttt gcgggcgcct cctcaccggc
     2101 caccgtct
//
LOCUS       DAOPTR010000754         2104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_41404_length_2104_cov_3.169839, whole genome shotgun sequence.
ACCESSION   DAOPTR010000754 DAOPTR010000000
VERSION     DAOPTR010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41404_length_2104_cov_3.169839"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="PKI05_12860"
     CDS             complement(<1..1106)
                     /locus_tag="PKI05_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53136.1"
                     /translation="MYQSSQTPLWFSLFIFPALGLLFAGCQPAKPVEKVGQQAGTGES
                     VAAPNEATAKAELADGGDKDASPDRTTVGQLWPSQTPSGTPWELPPSPGAPTANETVQ
                     SGWIVPDEPFSTTPSAGGQSQPVESPRLPSSLPGVSPRPETASATQDEAGPLPNPLRG
                     NQVPTAGDATIPQVMPNAPSSPLPAASDTQATPPRVATSKTSDRRLERPPFDPIKENG
                     QFFVGWTKPTVALVFTGNQDGYFEPCGCAGKERMKGGLSRRHTMIEQLRADGWPLVVM
                     DLGGLIKGFGKQTEVKFQITVDALRRIGYHAINLGRNDLRLPAPLLLSVIAPSSGTES
                     EFVSANVALFDFDTDQWTSRFRVVEAGGKRVGVTA"
     gene            complement(1172..>2104)
                     /locus_tag="PKI05_12865"
     CDS             complement(1172..>2104)
                     /locus_tag="PKI05_12865"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOA53137.1"
                     /translation="RNVGTAPLRISIGDSTCRCTVLNLDSAEIPPGESKPVTLEFNGK
                     DYFGPVTQRATILTNDPTRPQVTFTIKADVVKSIRVVPSEIVFSRVVKMQPAMGEAKI
                     YVFRDQPVQVKGIELANKDENAEFIDLDIQPLAPDELTVEPGAKSGYRVTVRLKPGLP
                     LGPIQQKIRVLTDQKDMPELEIPLAGRVESDVNITGVGWDSSRGMLHLGRINRSSGAE
                     RRVMLRLTPLVGENLKVEVDRTIPDFLQVTIEEPRYVEASLSLFVPITIRVPPGAGPA
                     NYFGLDEAHAGKVILKTNHPDAPEVKILVRFAVD"
BASE COUNT          445 a          615 c          578 g          466 t
ORIGIN      
        1 gccgtcacgc caacgcgttt ccccccagct tcgacaacgc ggaaccggct tgtccactga
       61 tcggtatcaa aatcgaatag ggcaacattg gctgaaacga actcactctc cgttccgctg
      121 cttggtgcaa taacagacaa taacaacggt gccggtagtc gcaagtcgtt gcgacccaaa
      181 ttgatagcgt gatacccgat tcgccttagg gcatcgaccg tgatttgaaa cttgacctct
      241 gtctgcttgc caaagccttt aatgagacct ccaaggtcca tgacgacgag tggccaacca
      301 tcggcccgaa gctgttcgat catcgtgtgc cgtcggctca agccgccttt catccgctcc
      361 ttgccagcgc aaccgcacgg ctcaaaatat ccatcttgat taccggtgaa gacaagcgcc
      421 acggttggtt ttgtccaacc gacgaagaat tggccattct ccttgatggg atcaaaaggt
      481 ggccgctcta accggcggtc cgaggtcttg gatgtagcca cccggggtgg cgtcgcttgt
      541 gtgtcggagg ccgcaggtag aggcgagctc ggagcgttgg gcatcacctg gggaatggta
      601 gcgtctcccg cggtgggaac ctgattgccc cgcagaggat tgggaagagg ccccgcttcg
      661 tcttgggttg ccgaagcagt ttctggcctg ggtgaaacac cgggcagcga cgatggcagc
      721 cgcggggact ccaccggttg agattgccca ccggctgatg gcgtcgtgga aaacggctca
      781 tccgggacga tccagccact ctggacagtt tcattagccg ttggggcacc tggactgggt
      841 ggcagctccc aaggtgtccc gctgggtgtc tgcgatggcc acagttgacc cacagttgtc
      901 cgatcgggcg aagcatcttt gtccccacca tcggcgagct ccgccttggc ggttgcctca
      961 ttgggggctg caacagattc tcctgtcccc gcttgctggc caactttctc cactggtttt
     1021 gctggctggc agcctgcgaa taacaatccc agcgccggga aaatgaacag actaaaccac
     1081 aagggcgtct gagaagattg gtacatccgt cacgttctcc gtacaagggg atccgtcctg
     1141 aaaaatgcac gcccgttcgc aagccgtgca ctcaatccac ggcaaagcga acgaggatct
     1201 ttacctcagg cgcatccgga tggttggttt taaggatcac ttttcccgca tgtgcttcgt
     1261 ccaaaccgaa gtaattagcc ggcccggccc cgggtggaac acgaatcgtg atgggaacga
     1321 acaaactcag cgaggcctcc acgtaccggg gttcctcaat tgttacttgt aagaaatcag
     1381 gaattgtacg atcgacctcg acctttaggt tttccccaac gagaggtgtc aggcggagca
     1441 tcactcgccg ttccgcccct gagctccgat tgatccgccc aaggtgtaac atcccgcggg
     1501 aactatccca gccaacaccc gtgatgttga catcgctttc cacccgacca gccaatggaa
     1561 tctccaactc aggcatatcc ttttggtcgg tcaacacgcg gattttctgc tgaattggcc
     1621 ctaaaggcaa gcctggtttt agccgaaccg tcacacggta gcccgatttg gccccaggtt
     1681 ccacagtaag ttcgtcgggg gcaaggggct gaatatccag atcaataaac tcagcgttct
     1741 cgtccttgtt tgccaactca atccctttga cctggacggg ctggtcccgg aatacataaa
     1801 tctttgcttc ccccattgcc ggctgcatct tgaccacccg actgaagacg atctcgctcg
     1861 gcacgacccg gatcgatttc acgacatcgg ccttgatcgt aaaagtgacc tgcggtcggg
     1921 tggggtcatt tgtcagaatg gtggcacgtt gtgtgaccgg gccaaaatag tccttgccgt
     1981 taaattccag cgttactggc tttgattcgc ccggggggat ctctgccgaa tcgaggttta
     2041 gcaccgtgca gcgacaagta ctgtcaccaa tggaaattcg gaggggagcc gttcccacgt
     2101 tccg
//
LOCUS       DAOPTR010000755         2103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_41422_length_2103_cov_3.683594, whole genome shotgun sequence.
ACCESSION   DAOPTR010000755 DAOPTR010000000
VERSION     DAOPTR010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41422_length_2103_cov_3.683594"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            522..1166
                     /locus_tag="PKI05_12870"
     CDS             522..1166
                     /locus_tag="PKI05_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOA53138.1"
                     /translation="MYLSQAIVNLRTCQELRRHDHLLVGVAFTLIETIIALGLMAVVV
                     GAVTYSLVSSLQVAEENQDLVIAEGLAQDLMNEILNRRYCELGTSPYDPYLRPGSVEK
                     YPGTRELFDDIDDYNGWVEEPPQDRWGIPLGEEDGVGGRRPYPARAASLSGFRREVIV
                     SYVSSQDFSKTLPFGSVSDYRKIEVIVSRRYPNNVVRQLVKLTRVVGHVPSEQF"
     gene            1144..2091
                     /locus_tag="PKI05_12875"
     CDS             1144..2091
                     /locus_tag="PKI05_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53139.1"
                     /translation="MFLASSFDSRITRSLRWVRHSDRRDGQAEKVGLRRKPLASDHDP
                     HRRGISHQTPKSYGVGFTLVEMLAAVALTGLIVVGIVAFAQLAEDSFATGQDSSETIQ
                     IARTCFQQIESMLNAAWGNELFPGALVVPRLVSGTQFPETLVIWCPVTGQPRNPDGLP
                     CVDELVVLSPDSSRPHELRKYRVSNNSATVPSPSNVSAWQSLIHGIQSQSPAQSQLLT
                     DRLRTFTVNNVTLGAIRFRVETVPSDSEWADSSVTWSTLSWPQGLYGPNRGVRRVSVH
                     TEIQLRKKTGSLTPQPEASCSFWHSHAYLYTVRKEKRLQ"
BASE COUNT          489 a          554 c          560 g          500 t
ORIGIN      
        1 gccacagctt gggctagcca gtccggcaag ccccacgcca cgaacatatc gtggcctttc
       61 gcctgataga actagagatg tcatcccaag gcccaagaca atggccagga aaattagtct
      121 catcttcata ggttggtcct cgttctttct tgttctgttt cgctcgattc acatcgggaa
      181 ttgtgaccgc cgggtcattc gcctgcgttg cgtccaaaaa cggcccccaa aacccttatt
      241 actatcatcg gatagaagcg ctggcacaat ccaacgtttc gtcaaaggcg tatcgggtta
      301 agtttcgcat ttccacaata ttaccaagat atggcggttt tggcgaaacc ctcgacgtcc
      361 tcctaccagc ggcaaccggg ctcaacaaga tgcattttat gggtaatagg ccccttgggg
      421 gatgcccaag ttctccggca agataggtcg ccccgaagat tgcatcgcgg cctatattta
      481 tgtgacatcg taaacgtgac ccgttttatg accagaaaaa aatgtatctg agtcaagcga
      541 tcgtcaattt gcggacatgc caggaattac gccgacacga ccacctgttg gtgggggtag
      601 cctttacgtt gattgaaacc atcatagctc tcggcttgat ggccgttgtg gtaggtgcgg
      661 tgacgtattc gttggtaagt tctttgcaag tggctgaaga gaatcaagac ttggtcatcg
      721 ccgaggggtt agcccaagac ttgatgaacg aaatactcaa ccgccgttac tgtgaattgg
      781 gaacgtcacc gtatgatccc tatctccgcc cgggcagtgt cgaaaagtac ccgggaacgc
      841 gcgagctttt tgacgacatt gacgattata acggctgggt ggaagagcca ccgcaagacc
      901 gctggggaat tcccctcgga gaggaagacg gggtgggcgg aaggcgccca tatcctgcta
      961 gagccgcgag cctcagcggc ttccgtcgtg aggtcattgt cagttacgtt agttcccaag
     1021 atttctcgaa aaccctacct tttggttcgg tttcggatta tcgaaagatc gaagtcatcg
     1081 tcagccgtcg gtatcccaac aacgtcgtgc gccaactggt caagcttaca cgagttgtcg
     1141 gccatgttcc tagcgagcag ttttgattcc cgcattaccc gatcgttacg ttgggtacgt
     1201 cactccgatc gacgagacgg ccaggccgag aaggttggtc tgcgacgaaa gccgttggcg
     1261 tcggatcatg acccacatcg tcgcggaatc tcgcatcaga cgcccaaatc ctatggggtg
     1321 gggttcacgc ttgtcgaaat gctcgctgcc gtcgccttga ctggtctaat cgttgttggc
     1381 atcgtggcgt ttgcccaact ggccgaagac tcgtttgcaa ctggccaaga tagttcagag
     1441 acaatccaga ttgcgcgaac atgttttcag cagatcgaat ccatgctgaa cgctgcttgg
     1501 ggtaatgagc tattcccggg tgcacttgtg gtgccgaggt tggtttcggg aacacagttt
     1561 ccagagacac tcgtcatctg gtgcccagtt accggccagc cgcgaaatcc agatggtttg
     1621 ccgtgtgtcg atgaacttgt ggtgctcagt ccggatagtt ctcgacccca tgaattgaga
     1681 aaatatcgcg tctcgaacaa cagtgctaca gtacctagtc cgtcaaatgt ttcagcgtgg
     1741 caatcgctca ttcacggaat acaaagtcaa tcgcccgcac aaagtcagct tctgactgat
     1801 cggctacgaa cattcacggt taacaatgtc acgcttgggg ccatccgttt ccgagtggaa
     1861 acagtcccca gtgatagtga atgggccgac tccagcgtta catggagcac actttcctgg
     1921 ccacaggggt tatacggtcc aaatcgcgga gtccggcggg tttcggtgca cacagagata
     1981 caactccgca agaagacggg ctcgctcaca ccgcaaccgg aagccagttg cagcttctgg
     2041 cattcccatg cgtatttgta cacagtgcgg aaagagaaga gactccagta gctgttctca
     2101 tga
//
LOCUS       DAOPTR010000756         2102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_41446_length_2102_cov_3.597460, whole genome shotgun sequence.
ACCESSION   DAOPTR010000756 DAOPTR010000000
VERSION     DAOPTR010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41446_length_2102_cov_3.597460"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="PKI05_12880"
     CDS             <1..1204
                     /locus_tag="PKI05_12880"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 140 protein"
                     /protein_id="HOA53140.1"
                     /translation="YLKDRASKGFTVIQAVVLAELDGLHTPNPYGHVPLIDDDPTRPD
                     IHPGSDNDYWDHVDWIVNRAEQLGLFIGMLPTWGDKWNKKWGVGPEIFTPENARIYGE
                     FLGKRYRDKPIIWILGGDRNPENERHLAIIRAMAAGLRTGDQGNHLMTYHPQGGANSA
                     NWFHEDDWLAFNMFQSGHGRINNPNYLMTLANYERKPVKPTLDGEPRYEDHPVNWRAE
                     QGWFDDWDVRQGAYWSMLSGACGHTYGSHNIWQMYEPPRKPVSAARTPWKKALHHPGS
                     TQMGYMKQLFLLRPYYRLIPDQQLIAGQEKSEGEHRRAARAEDSTFAVIYLPTGGTIR
                     VITARLKGQNLHVSWFDPRIGKVVRSETVRKEAEIPLAAPSAGRGNDWVLVLDSDQEN
                     LPPIRIGE"
BASE COUNT          487 a          558 c          590 g          467 t
ORIGIN      
        1 gtacctcaaa gaccgcgcaa gcaaaggctt tacagtcatc caggcagtag tattggcaga
       61 attggatggc cttcacacgc cgaaccctta cggtcatgtt ccgctgattg acgatgatcc
      121 cacacgaccc gacattcacc ccggtagcga taacgactac tgggatcacg tggactggat
      181 tgtcaatcgg gcggagcaat tggggttatt catcgggatg ttgccgacgt ggggcgacaa
      241 gtggaacaag aaatggggtg tcggtccgga aatcttcacg ccggagaatg cccgcatcta
      301 cggcgagttt ctcggcaaac gatatcgtga taaaccgatc atttggatcc tggggggtga
      361 tcgaaacccg gagaatgaac gtcacctggc catcattcgc gcaatggcgg cggggcttcg
      421 taccggtgat caaggcaatc acctaatgac gtatcatccc cagggtgggg cgaactcggc
      481 caattggttt cacgaagatg attggctcgc cttcaacatg tttcaatctg gacatggtcg
      541 catcaataat cccaattact tgatgacctt ggccaattat gaacgcaaac ctgtgaaacc
      601 gacacttgat ggtgagcccc gctacgagga ccatcccgtt aattggcggg ctgaacaggg
      661 atggttcgat gattgggacg tccgccaggg tgcgtattgg tcgatgcttt ccggcgcttg
      721 tggtcacacc tatggtagcc acaatatctg gcagatgtat gagcccccgc gaaagccggt
      781 gtccgccgcc cgcacgcctt ggaaaaaggc cctccatcat cccgggtcaa cacaaatggg
      841 atacatgaaa cagttgtttc tccttcgccc ttattaccgt cttatcccag accaacagtt
      901 gatcgcaggc caagagaaga gtgaaggaga gcaccggcgg gccgcgcgtg ctgaggatag
      961 cacctttgcc gtcatttatc ttcccacagg agggacgatc cgcgtgatca cggctcgcct
     1021 caaagggcag aatctgcatg tctcttggtt tgatcctcgg atcggaaagg tggttcgttc
     1081 ggagaccgtt cgcaaggaag ccgaaatccc tctggcggct ccctcggcag gccgcggaaa
     1141 cgactgggtt cttgtactag atagtgacca ggagaatctt cctccgataa gaattggcga
     1201 atgactatgg agcaccgcgg cgtaggcgta aggcctgtgc ctaccgttgt tcgctatgga
     1261 cggcaccgaa caaaacccca gtttcgcgat gggggttggt gatcccgcaa cacatgagga
     1321 tttagggtta accggcagca gttgcctcgt tttttcatgc cgcgtgccgt atttcaccaa
     1381 attttcaagg gcgcgccgtc gcgcccatcg atcgcctggg accccaattt caactgacgg
     1441 gaccgtaggt ccgcatcgta ccaaccggct tgccgacgag ttatgcggcc acgacggtgt
     1501 gtggccctac acctgggcaa gtgggccacc gcagtgtgga gtctccaagg tcaatgcgcc
     1561 ggacgggaac aaggccaccg gcgccaaaaa tttctccgat tcttgtcgat cccggtcatt
     1621 gatggtagaa tttggccaaa tgggatttgc ggccgcttgc agccatatct gctaaagtgc
     1681 catcgtcgct ctttggaggt tggtttgaaa gcgacgatcc atcacgaatc tcgccagccg
     1741 cgacgcccgt tcgtcgcggt ttttctttag cataagcagg cgagtagcga gttggttcgg
     1801 gccgccgtgt cagtgcatgg gtagcgttag tgaaaacaac agaacagaaa gcggtatctt
     1861 ctatgacgaa cgagccaact gcctcgaaag ggtcaaagga ctctgtcggt ttctccacat
     1921 tgtgtgtgca cggcggggaa gatcgtgtca agccatgtta ctccatgact gacccgatct
     1981 tctgtacggc aacttacact ttcgagaaca cggcctcttt gatcgaatat gtaacgaaaa
     2041 agcttccccg agaagagtat ggccgttatg gcaatcccac ggaaaaggtt ttagaacgca
     2101 aa
//
LOCUS       DAOPTR010000757         2101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_41480_length_2101_cov_2.715543, whole genome shotgun sequence.
ACCESSION   DAOPTR010000757 DAOPTR010000000
VERSION     DAOPTR010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41480_length_2101_cov_2.715543"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PKI05_12885"
     CDS             <1..475
                     /locus_tag="PKI05_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53141.1"
                     /translation="SGRTASQTVVTGEAIGQLLPRSWQIDTHTLSLETPWGERVEESL
                     QLTGQYLRWSYGNTEHSGVYRAEARLKSGNTQTTVFVANPPVAESDIRPWNFVGFVER
                     VLPQAKYVSELESLRITPTNVSSALRASPLSRWLLCFVLVVLLGELYLGRHTPGT"
     gene            573..>2101
                     /locus_tag="PKI05_12890"
     CDS             573..>2101
                     /locus_tag="PKI05_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53142.1"
                     /translation="MPNRFTDGTSQSVRVTGDWPWPVWITIVLIILIFFLIYRSYKKT
                     RGDLSWKQRHLLLALRCITSAIVFLMLAQWILVTEQLGMPILAIILDNSLSMRIKDVD
                     RQPEQGVVKANSGDPFDRERWNQAVDVLCGGPSPLLDELAQDYRLKVYLLDGKTELKG
                     PVTDIASTLRVMKPERATTPLGKTIHEVIDQLRGQPPVGVIVLTDGVVNEGPGLEDAA
                     KRASQELVPLYFVGFGAEKGSPDLSVQSWVMEDTVFLGDHVSLQAQLVAKNTAAKEAR
                     VRLTDITHQQILEEERVPISAETTVFTMIFKAEQAGEIELRLECLPIQDEIDLRNNAL
                     SRRILVKKDTIRILMVEEVPRFEYRCLRNALIREPGFDLTTVLLGADPEYVEEMKGAM
                     LAVPTSRDELWSYDVIILGDVAPNRLSPSTLEAVVAFVEEKDPPGGLILVGGPHAMPH
                     EYNQSVLDKVLPCAVGVSPANIGQPKTAAHRLAVTREGLVMPWLAFGRSPTETERVWH
                     S"
BASE COUNT          478 a          515 c          597 g          511 t
ORIGIN      
        1 aagcggtcgc acggcttccc aaaccgtggt tacgggcgaa gccattggcc agctcttgcc
       61 caggagttgg caaatcgaca cgcatacgtt gtctctcgaa accccctggg gagaacgcgt
      121 ggaggagagc ctgcaactaa ctggccaata cctacgctgg tcgtatggca ataccgagca
      181 tagtggtgtc tatcgtgccg aggctcgctt aaaatcggga aacacgcaaa ctacggtctt
      241 cgtagcgaat ccgccggttg cggaatccga tattcggcca tggaactttg ttggtttcgt
      301 agaacgggtg ctgccccaag cgaagtatgt gtcggagcta gagtcgctga gaatcacccc
      361 cacgaatgtc agttcggcct tgcgggcatc acctctctcc cgatggctcc tttgctttgt
      421 ccttgttgtg ctcttgggag aattgtattt aggccgtcat acaccgggta cataagcgca
      481 agtctaggag tttgatgtga cggtatttca caaaatgttg cgtctgcttt cttccaatgt
      541 ccgattggat taaccgcttg gttcatacct taatgcctaa tcgttttacc gacgggacga
      601 gccagtctgt gcgggtcacc ggggattggc catggcctgt ttggataacg atcgttctca
      661 taattctcat ctttttcttg atttatcgtt cctacaaaaa aacacgcggt gacttgagtt
      721 ggaaacagcg tcatctcctg ttggctttga ggtgcatcac tagtgccatc gttttcctaa
      781 tgcttgccca atggatctta gtgacagaac aactgggcat gccgatcctg gcgattattt
      841 tggacaactc gttaagcatg cgcataaagg acgtggatcg gcaacctgaa caaggagtag
      901 tgaaggcaaa ctctggggat ccttttgatc gagagcgctg gaatcaagcc gttgacgtgc
      961 tctgcggtgg gccctcgccg ctactcgatg agttggcaca ggactatcgc ctcaaggttt
     1021 atctactcga cggaaaaact gagctgaaag ggccggttac cgatattgcg agtaccttac
     1081 gcgtgatgaa gccggagcgt gccacgaccc ctctggggaa aacaattcat gaagtgattg
     1141 atcaactccg tgggcaaccg ccggttggcg tgatcgttct taccgatggg gtggtcaatg
     1201 aaggcccagg ccttgaggat gccgctaaac gggcaagtca agagctagtt cccctctact
     1261 ttgtgggctt tggggcggag aaaggctcgc cggatttgag tgtgcagagc tgggtcatgg
     1321 aagataccgt gtttttgggc gatcatgttt cccttcaggc acagttggtt gcaaaaaaca
     1381 cggctgccaa ggaggcgcgg gttcgtctta ccgatattac gcatcagcag atcctggaag
     1441 aggaaagggt ccctatctcc gcagaaacaa cggttttcac catgatcttc aaggccgaac
     1501 aggccggaga aatcgaatta cgtttggagt gtttgccgat ccaggatgaa attgatctgc
     1561 ggaataacgc gttgagcaga agaatcctgg taaagaaaga taccatacgt atcctcatgg
     1621 ttgaggaggt gccgcgtttt gaatatcgat gccttagaaa tgccttgata cgcgagccag
     1681 gctttgattt aacgaccgtc cttctgggag ccgatccgga atacgtggag gaaatgaaag
     1741 gggccatgtt ggcggttcca acctcccgcg acgagttgtg gtcctacgat gttatcatct
     1801 tgggagacgt ggcccccaat cgactttcgc cgagtacact ggaggctgtg gtggcctttg
     1861 tggaggagaa agatccaccg ggtggactaa ttctggtcgg tggaccacat gccatgcccc
     1921 acgagtacaa tcagtcagtc ctcgacaagg tcctaccttg cgccgtaggg gtatctccgg
     1981 cgaacatcgg acagccgaag acggcagccc ataggctggc cgtcactcgg gagggcctag
     2041 ttatgccttg gctggccttt ggaagatcac ctaccgaaac cgaacgggtg tggcattcgt
     2101 t
//
LOCUS       DAOPTR010000758         2094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_41643_length_2094_cov_3.422266, whole genome shotgun sequence.
ACCESSION   DAOPTR010000758 DAOPTR010000000
VERSION     DAOPTR010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41643_length_2094_cov_3.422266"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            793..>2094
                     /gene="malQ"
                     /locus_tag="PKI05_12895"
     CDS             793..>2094
                     /gene="malQ"
                     /locus_tag="PKI05_12895"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOA53143.1"
                     /translation="MRESGILLHPSSLPSPMGIGDLGSSAYRFVEWLALAKQRYWQVL
                     PLGPVDDWGSPYSSPSAFAGNELLISLELLAEDGLLDSGELKQQFDHTTDDAPVDYRA
                     VFRKKTAFLKQAFERFEAGRVRNAFDVLREEFELFCEQQSWWLKTYAIYQALVDLLGN
                     NDWPRWPRHVLNLGIDRLRRGGFDALGTDLPGELGKEYRFHCFAQFVFGRQWEQLHRF
                     ALERDVRIFGDIPIYVSYHSADVWANPQYFFLDEHYRPIAVAGVPPDYFNATGQLWGN
                     PVYRWEVIQSEGYRWWIDRFRAVFRLVDLARVDHFRGFAAFWSVPASHETAERGHWVT
                     APGEDLFTRLTTTWEGPWRQLPDGRKILPIVAEDLGHITPDVIALRRKFGFPGMAVLQ
                     FAFGASQVDAFLPDHVAVDTVMYSGTHDNDTSLGWFVHEILP"
BASE COUNT          446 a          546 c          554 g          548 t
ORIGIN      
        1 cgttcccgaa accttgaagg gcctgacggc ttggtaagac acccgtgaga atcagtgccg
       61 ccagcccccc catgaggata atatctgccg gcaagcgatt cgttgccagc aagacgaaaa
      121 ctaaaccgac gacaactaat gcaataacgg catcagtgcc catagacgtt ccccagtggt
      181 tttattcgtc tcaggataca aaagcatagg acggaagcaa aatcctttca caggggcttg
      241 ttacccgtca gctttcagcg tcctgtctta tttcggtttg gaccgcacga cggcggcccc
      301 atttgacatc tagagacagg ctccggcgag ccgtaaatca ccaaagtgac gacggtgtat
      361 ttctaaatcg aggcaaacat gatcttttct atcatgatag tatactttcc atttttaggg
      421 tagtccgttg gggcctccgc tccttagagg acgttttgcc tgaaagtctc agcttctcga
      481 ctctcgatca tggagccgac aaagccggtt aaaatccgtg atctgactcc gctccttcct
      541 gttatacctt tcctaaattc cctattgttg tcatcctgca agctgatgcc caaattaggg
      601 gctcgtatgt ggttcaaagc cccctgtgtc tcggtcccgc atcccataga atgagttatg
      661 gacgaaatcc aggaaaaccg ctataagggt tgatttcttc tggtccatcc ctcctgtcaa
      721 gttcgtgggg atgcgtgggt gtgttgaagc gccattgcga ttgcattcca tttacggtgt
      781 tgatttcttc aaatgcgtga aagcggaatc ttacttcatc cgagctcact tcccagccca
      841 atggggattg gtgatcttgg ttcttctgca tatcgctttg tcgagtggct tgctctggca
      901 aaacagcgat attggcaggt ccttcccctg ggacccgtgg acgattgggg gtcaccatat
      961 agttctccct cagcgtttgc tgggaacgaa ctgttaatct cgctggaact ccttgccgaa
     1021 gacggactcc tcgatagcgg agaactaaaa caacaatttg atcacacaac ggatgatgcg
     1081 ccggtcgatt atcgagcggt gtttcgcaag aaaacggcat tcttgaaaca agccttcgaa
     1141 agatttgagg ccggccgcgt cagaaacgca ttcgatgtac tccgggaaga atttgagctt
     1201 ttctgcgagc aacagtcttg gtggctcaag acgtacgcga tctatcaagc attggtggac
     1261 cttctcggaa acaacgattg gccgcgttgg ccgcgacacg tgctcaacct aggtatcgat
     1321 cggcttcgac gtggcgggtt cgatgctcta ggtacggatc ttcctggcga gttggggaaa
     1381 gagtatcgct ttcattgttt tgcccagttt gttttcggcc gtcagtggga acaattacac
     1441 cggtttgcgc tcgagcggga cgtccggatt tttggagata tcccaatcta tgtctcctat
     1501 cacagcgccg acgtctgggc caatccccag tatttttttc ttgacgaaca ttaccgaccg
     1561 atagcggttg ccggggtacc gccggattac ttcaatgcca cgggccaact ttggggaaat
     1621 cccgtttacc gatgggaggt gattcaaagt gagggctacc ggtggtggat cgatcggttt
     1681 cgcgctgtgt tccgcctggt tgaccttgcc cgggtcgatc attttcgtgg gtttgccgcc
     1741 ttttggtcgg ttcccgcttc gcacgaaacg gccgagcggg gacactgggt gaccgcaccg
     1801 ggtgaggacc tctttacccg gcttaccacg acatgggagg ggccgtggcg acagttgcct
     1861 gacggtcgga aaatcctccc tattgtggcg gaggacctcg gccatattac cccagacgta
     1921 attgccttgc gacgcaaatt tggtttcccg ggcatggccg tgctccaatt cgcctttggg
     1981 gcatcccaag tcgatgcttt cttgcctgac catgtggctg tggataccgt gatgtactcc
     2041 ggtacacatg acaatgatac aagccttggt tggtttgttc acgaaatctt gccg
//
LOCUS       DAOPTR010000759         2093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_41664_length_2093_cov_3.318449, whole genome shotgun sequence.
ACCESSION   DAOPTR010000759 DAOPTR010000000
VERSION     DAOPTR010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41664_length_2093_cov_3.318449"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="PKI05_12900"
     CDS             <1..882
                     /locus_tag="PKI05_12900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA53144.1"
                     /translation="GGIPKERVTFRRNGNHEAVVNGREEVVVADGLVKKFGHFTAVDH
                     VSFRVRRGEVFGLLGPNGAGKSTTFRMLCGLLAPTEGRATVAGVDLRKARAEARRHVG
                     YMAQKFSLYGNLTVKQNLQFFGGVYGLRGDNLRNAIDWALEELELKPFADQNTIQLPL
                     GFKQRLALACAIIHRPDVVFLDEPTSGVDPLARREFWLRIDQLVEQGAAVIVTTHFMD
                     EAEYCDHLALINRGRIIAMDTPDAVRAMVQSPEHPNPTLEEAFIELLIRFGEQEGVET
                     NPNTLLNDEKRSEEKTK"
     gene            879..2039
                     /locus_tag="PKI05_12905"
     CDS             879..2039
                     /locus_tag="PKI05_12905"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA53145.1"
                     /translation="MNAVNRQNVRLSARRFRLRCVLALARKESYQIVRDPSSLVIAFL
                     LPVVLLFLFGWGISLDLGSIPIGLVVESPSRDAKRLVASLRGSRYFIVREANHRQEME
                     PLLVGGRIKGLVIIPQDFAANLSRGSATVQILVNGSEANTAEIVRSYLENVLQTEHRH
                     RAEETGNRLIGDTIHLESRVWYNPNLDSRAALLPGSIAVIMTLIGTILTSLVVAREWE
                     RGTMEALLASSITRGEFLLGKFIPYFALGLTAMMLVALVSVIIFHIPFRGSWGLLIVV
                     SAIYLTVALGLGLFISTATRNQFVATQAALVVGYLPSFILSGLVFEINSMPTPIRLLT
                     HILPPRYFVSALRTLFLAGNVWEIVLQDTAILAVFATILWMLTIRKTRLTLE"
BASE COUNT          450 a          507 c          605 g          531 t
ORIGIN      
        1 ggaggcatcc ccaaggaaag ggtaacattt cgaaggaatg ggaaccacga ggccgtcgtg
       61 aacgggcggg aggaagtagt cgtagccgat ggcctggtaa aaaaattcgg tcacttcacc
      121 gctgtggacc acgtttcctt ccgcgttcgg cgaggagaag tctttggtct attaggacca
      181 aatggggctg gaaagagtac caccttccgc atgctttgcg gtttacttgc gccaacggaa
      241 gggcgagcaa cagtcgcggg ggtggacctt cgcaaggcac gggcggaggc acgcagacac
      301 gtcggttata tggcccaaaa gtttagcctt tacggtaatc tcacagtcaa acagaatctg
      361 caattctttg gcggggtgta cggactgcgg ggagacaatt tgcgcaatgc catcgattgg
      421 gccttggaag aattggagct taagccgttt gccgaccaga acacgatcca gctcccactg
      481 ggctttaagc agcggctggc tctggcgtgc gccatcatcc atcgcccgga tgtggtcttt
      541 ttggatgagc caaccagcgg cgtggatccc ttagcccggc gagagttctg gctccgcatc
      601 gatcaacttg tggaacaggg agctgccgtc attgtgacca cacacttcat ggacgaagca
      661 gagtattgcg atcacctggc cctcattaac cgcgggcgga ttatcgccat ggatactccc
      721 gatgccgtga gggccatggt tcaatctccg gagcacccga atccgacact ggaagaagct
      781 tttatcgagc ttcttattcg gtttggagag caagaagggg ttgagactaa ccccaacacg
      841 cttttgaatg atgaaaaacg atctgaagag aagaccaaat gaacgcggtc aatcgacaga
      901 acgtccgact gagtgcgaga cgctttcgcc ttcgctgtgt tttggcgtta gcgcggaaag
      961 agagttatca gatcgttcgg gatccgagca gcctggtgat cgccttcctc ttgccggtgg
     1021 tcctcttgtt tctctttgga tgggggattt cccttgacct gggatcaata ccgattggac
     1081 tcgtggtcga atcaccctcg cgtgatgcga agcggctggt ggcttccctg cgcggaagcc
     1141 gttatttcat cgtgagggag gccaatcatc ggcaagagat ggaaccgctc ttggtgggcg
     1201 gacggattaa gggattggtc atcattccgc aggatttcgc agcgaacttg agtcgtggat
     1261 cggcgacagt ccagattctt gtgaacggta gtgaagccaa cactgcggaa attgtgagga
     1321 gttatttgga aaacgtcctg caaaccgagc atcggcatcg tgctgaggaa acaggtaacc
     1381 gactaatcgg ggacaccatt catttagaga gtcgggtttg gtacaacccg aatttggact
     1441 cgcgggctgc tttattgcca ggttccatcg ccgtcatcat gactcttatt ggaaccattc
     1501 ttacgtcatt ggtcgtggcg agggaatggg aacgtggcac aatggaggcc cttctcgcca
     1561 gttcaatcac tcgaggcgag tttctgctcg gcaagttcat tccatatttt gccctcgggc
     1621 tgacggcgat gatgcttgtg gccttggttt cggtcattat ctttcacatt ccgtttcggg
     1681 gatcctgggg gcttttaata gttgtttcag ccatttattt gacggttgcg ctggggctag
     1741 ggcttttcat ttcaacagca acgcgtaatc aatttgtcgc tacccaggcg gcactcgttg
     1801 tggggtattt gccctctttc atcctttctg gtctcgtatt cgaaattaac agtatgccga
     1861 cccccattcg attactcact cacatattgc cgccacgcta ctttgtgtcg gcgttgcgga
     1921 cacttttttt agcaggaaat gtgtgggaga tcgtgctgca ggatacggcc atcttggcgg
     1981 tgtttgccac aatcctttgg atgttaacca ttcgtaagac gcgtttgact ttggagtaag
     2041 ggaaggttca tgctggagcg catccggcgt ttggtcatta aagagtttct ggc
//
LOCUS       DAOPTR010000760         2089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_41765_length_2089_cov_2.117994, whole genome shotgun sequence.
ACCESSION   DAOPTR010000760 DAOPTR010000000
VERSION     DAOPTR010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41765_length_2089_cov_2.117994"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /gene="rpsM"
                     /locus_tag="PKI05_12910"
     CDS             complement(<1..177)
                     /gene="rpsM"
                     /locus_tag="PKI05_12910"
                     /inference="COORDINATES: protein motif:HMM:NF012633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOA53146.1"
                     /translation="MPRLLGVDIPGNKPTVIALRYIYGVGPAIAKELCYKAGIDPHIR
                     ANQLREDELARLATL"
     gene            complement(233..346)
                     /gene="rpmJ"
                     /locus_tag="PKI05_12915"
     CDS             complement(233..346)
                     /gene="rpmJ"
                     /locus_tag="PKI05_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005987264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOA53147.1"
                     /translation="MKVRASVKRLCSNCKVVRRQGRIYVICSNPRHKQRQG"
     gene            complement(497..1318)
                     /gene="map"
                     /locus_tag="PKI05_12920"
     CDS             complement(497..1318)
                     /gene="map"
                     /locus_tag="PKI05_12920"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOA53148.1"
                     /translation="MITLRSRSEILSMRRAGLVVWKALELVKSMVRPGLMTKEIDQAV
                     EDFFKRSGAQPLFKGVPGRVPFPAVTCISVNEEVVHGIPGDRQIKEGDIVSVDTGCRV
                     EGWCGDAAVTIPVGRPAPEVLKLVEVTRQTLDLAIELMGRSRLWSEVAAAMQRYVEKH
                     GFSVVEAFVGHGIGRSMHEEPQVPNFVNALFLKKGDFALQPGLVIAIEPMVNLGTKKV
                     RLCRDHWTQVTADGKPSAHFEHTVAMTEFGPYVLTGPLGPMDEKLGFDPDRHHFA"
     gene            complement(1372..1935)
                     /locus_tag="PKI05_12925"
     CDS             complement(1372..1935)
                     /locus_tag="PKI05_12925"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOA53149.1"
                     /translation="MRLVFIGPPGAGKGTQAERIVSRYMIAHLSTGDMLRAARNAKTP
                     LGIQADAYMSRGELVPDDLIIDLIRERLAADDCQRGYLLDGFPRTIAQAEALDKMLGE
                     KKTPLDVVLELRVPEEELFRRLAGRGRADDKPEVIRQRLVAYREQTAPLLDYYQEKGL
                     LKTVDGLGTVDEVFERIRSILDEFAGA"
BASE COUNT          460 a          659 c          491 g          479 t
ORIGIN      
        1 caacgtggcc aggcgcgcca gttcatcctc tcggagttga ttcgcacgaa tgtgaggatc
       61 aatccccgcc ttgtagcata actctttggc gatggctggc ccaactccgt atatgtaccg
      121 caacgcgata accgtgggct tatttccagg aatatcaacc ccaagcaacc gtggcatatt
      181 tgccgctcct ggtgatcaat cagtcaaccg tttcttctat ttttccaaat aatcaccctt
      241 gccgctgctt gtgacgcgga ttggagcaaa tgacgtaaat ccggccctgc ctgcggacaa
      301 ctttacaatt actgcataaa cgcttgacac tcgctcgcac tttcatattc tgaatccccg
      361 ttatctcgtg gttggcagtg ctgtcgaaac ctttagtata gagcgccttg ctttggcgac
      421 tcaaggcccc cgcaggccgc cggtttgctt agtcttttcc ctttatttcc tatttcctcc
      481 gcgaccgctc ccgaaatcac gcaaaatgat gtcgatcagg atcaaaaccg agcttctcat
      541 ccatggggcc taatggcccg gtcaaaacat atgggccaaa ctcggtcatg gccaccgtgt
      601 gctcaaaatg agcactcggt tttccgtcag ccgttacctg ggtccaatga tcccggcaga
      661 gccgtacctt tttagttccc aaattgacca tcggttcgat ggcaatcacc aggccgggct
      721 gaagggcaaa atcgcccttt ttcaaaaaca acgcgtttac aaaattggga acctggggtt
      781 cctcgtgcat ggatcggccg attccgtggc ctacaaacgc ctcgactaca ctgaatccat
      841 gcttttcgac gtaccgctgc atggccgcgg ccacttcact ccacaatcga cttcgtccca
      901 tcagctcgat ggcaaggtca agggtctgcc gagtgacctc caccagcttg agcacttccg
      961 gagccggacg gcccacaggg atcgtcacgg ccgcatcgcc acaccagcct tcgacacggc
     1021 aacccgtgtc cacgctgacg atatcaccct ccttgatctg gcggtcgccc gggatgccat
     1081 ggacgacctc ctcattcacg gagatgcagg tcacggcagg gaaggggaca cgtccgggaa
     1141 cacctttaaa cagcggctgg gcaccagacc gtttgaaaaa atcctcaacg gcctggtcga
     1201 tctcctttgt catcaggccc ggtcgcacca tggacttgac taactcgagg gctttccata
     1261 caacaaggcc cgcccttcgc atgctgagaa tttctgatcg tgaccgcaac gttatcacac
     1321 ctggctcact cccagtccca ctggtgcgac tctcctggcc caactcaccc ctcaagctcc
     1381 cgcaaactcg tcgaggatac tccggatccg ctcgaagacc tcgtccacgg tcccgagacc
     1441 atcgacggtc ttcaacaatc ccttctcttg gtagtagtcc aacaaggggg ccgtctgctc
     1501 tcggtaagcg acaagccgct gtcggatgac ctctggtttg tcatcggctc gcccacgacc
     1561 ggccaggcgc cggaacaatt cttcttcggg aacccgcaat tccaaaacga cgtccagtgg
     1621 cgtctttttc tccccgagca ttttgtctaa agcttcggct tgggcgatgg tccttggaaa
     1681 gccgtcgagc aggtaaccac gctggcagtc gtccgcggcc aatcgctctc gaattaaatc
     1741 aataatcaaa tcatcgggga ccagctcgcc ccgcgacata taagcgtcgg cttgaatccc
     1801 caatggagtt tttgcatttc tcgccgcccg tagcatgtcg ccagtggaaa ggtgggcgat
     1861 catgtaccgc gagacgatcc tttccgcctg cgtgcctttt cccgcgcccg gtggaccaat
     1921 gaataccaat cgcatcgctg gataatccta aaaccgactt gccaatggta caacgaagga
     1981 gactctcacg cttacctgac tgcccagcga aaatactact cgagcaatcc cttgtaattc
     2041 cgcatgacga tgtggctatc gatcttttgt accaaatcca tagccacac
//
LOCUS       DAOPTR010000761         2085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_41864_length_2085_cov_2.738424, whole genome shotgun sequence.
ACCESSION   DAOPTR010000761 DAOPTR010000000
VERSION     DAOPTR010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41864_length_2085_cov_2.738424"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..943
                     /gene="tsaB"
                     /locus_tag="PKI05_12930"
     CDS             260..943
                     /gene="tsaB"
                     /locus_tag="PKI05_12930"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOA53150.1"
                     /translation="MKTLAIETTAPVGSLAFFNDGQVLRHWTLPEDRRSAATLAAELQ
                     RALQAIGWRPNDVELVAVSLGPGSFTGVRIGLVTAKVFAYATGAQLIGVDSLDAVAEN
                     IPGTYSPLVVLADAQRGEITAKLYVWEPTAGWLITKQSGLVTPQDWLPHLALEHQFWL
                     AGPGLYRFKESIGDQYRMVPEAFWSPTAVGVGKLAWKRFLAGQTDDIWTLAPIYYRAS
                     YAEEKRAPR"
     gene            complement(1029..2072)
                     /locus_tag="PKI05_12935"
     CDS             complement(1029..2072)
                     /locus_tag="PKI05_12935"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HOA53151.1"
                     /translation="MRRGTSFGAPTQAETELAELVCKIVPSIEKVRLVNSGTEATMSA
                     IRLARGATGRSKIIKFAGSYHGHVDSLLVAAGSSAATLGVPNSPGVTAGTAQDTLVLR
                     YNDVEGVQQVFEKMGEEIAAVIVEPVAGNMGCVPAKAEFLRKLRELTHKYGAILIFDE
                     VMTGFRVALGGAQSLYGITPDLTTLGKIIGGGLPVGAYGGKASIMDHVLPVGKVFQAG
                     TLSGNPLATAAGIATLRILQRENPYPLLEARSHQLEKGLTEIIAQTGLPARVQRCGSM
                     LTLFFTSEEIIDWDTASRCNTKQFARFFWGMLEEGVYLPCSQFEAWFVSTAHGETEIA
                     KTLEAASKVLKSL"
BASE COUNT          445 a          621 c          554 g          465 t
ORIGIN      
        1 tctgccaatt gagctacgct agcctcgcac gccgacgtct gatttaatat accggtctgg
       61 ccgaactctg caagccgacc cgacaaccgg ttttgcagtc gcggttccca atcccattat
      121 accgattcgc caggtgattt atcctcgggc gtaagcctgt ctcttggtgt accaatgggt
      181 ggtaagaacc cgccgccgag atggggttca ttacgatagg gctgtgatca tacagagggc
      241 acgaaatcat ttagcgcaaa tgaaaacact cgcaatcgaa acgacagccc ctgttggctc
      301 attagctttt ttcaacgacg gacaggtcct ccgccactgg acgcttcctg aagatcggcg
      361 gtccgccgca acactggcgg cagaattgca acgggccttg caagcgatcg gctggcggcc
      421 taatgatgtc gagctcgtcg cggtatctct ggggccgggc tcgtttaccg gagttcgcat
      481 cggcttggtg accgccaagg tctttgccta tgcaacgggg gctcaactca tcggtgtgga
      541 ttcgctcgac gcggtggccg agaacatccc tgggacatat tctcccctcg ttgtcctcgc
      601 agatgcccaa aggggagaga tcaccgcaaa gctttatgtt tgggagccaa ccgctggatg
      661 gctgattacc aagcaatcgg ggctagttac cccccaagat tggttgcctc atctggccct
      721 cgaacatcag ttctggctgg cagggcccgg tttgtatcgg tttaaagagt caatcgggga
      781 tcagtaccgc atggttccgg aagccttctg gagcccaacg gctgtcggtg taggtaaact
      841 cgcttggaaa cgctttctcg cgggccagac ggacgacatt tggacgctcg caccgatcta
      901 ttaccgcgca agttacgccg aggaaaagcg tgcaccgcga taatcctaca atagggccgc
      961 tctttgttgc tccccggctt tgggcgtcag tcaccagctc attgcatcac catcttggtg
     1021 ctgtgcgatt aaagagattt caagaccttc gaggccgctt ctaaggtctt ggcaatctct
     1081 gtctctccat gagccgtgga gacgaaccag gcctcaaact ggctgcacgg taggtaaaca
     1141 ccctcttcga gcatgcccca gaagaaccgc gcaaactgtt tggtgttgca gcgcgacgcg
     1201 gtatcccaat ctatgatctc ttcgctagtg aagaacaggg tgagcatcga accacatcgc
     1261 tggacacgag ccgggagacc tgtttgtgca atgatctcgg ttaaaccttt ttccaactga
     1321 tgggaccgag cttctagcaa aggataagga ttctctcgct ggaggatccg caacgtggcg
     1381 atacccgccg ccgtggccaa gggattgccg ctgagtgttc cggcctgaaa cacctttcca
     1441 acgggcagca catgatccat gattgaggcc tttccgccat aggccccaac cggcaatccg
     1501 ccgccaatga tttttcccaa cgtggtcaag tccggcgtga ttccatacaa actttgagcg
     1561 ccccccaagg caacgcgaaa gcccgtcatc acctcatcga aaatcaggat ggccccatat
     1621 ttgtgagtga gttcccttag tttgcggaga aactccgctt ttgctgggac gcaacccatg
     1681 ttccccgcca cgggctcgac aatcaccgcg gcgatttcct cacccatctt ctcaaaaacc
     1741 tgctggaccc cctcgacgtc gttataacgc agcaccagcg tgtcctgggc tgtccctgcg
     1801 gtcaccccag gagagttagg cacacccaga gtcgcggcag aactccccgc tgcaaccagc
     1861 aagctgtcca cgtggccgtg ataactcccg gcaaacttta tgatcttgct ccggccggtt
     1921 gcccccctgg ccaaacgaat ggcactcatc gtcgcttcgg taccggaatt aacaagtcgt
     1981 actttctcaa tggacgggac gattttacat accagttcag ccagttcggt ctcggcttga
     2041 gtgggtgccc cgaagcttgt gccgcggcgc atcgcggact caatg
//
LOCUS       DAOPTR010000762         2083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_41925_length_2083_cov_2.351085, whole genome shotgun sequence.
ACCESSION   DAOPTR010000762 DAOPTR010000000
VERSION     DAOPTR010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41925_length_2083_cov_2.351085"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..1547
                     /locus_tag="PKI05_12940"
     CDS             93..1547
                     /locus_tag="PKI05_12940"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA53152.1"
                     /translation="MSRVFNRRQFIKSAVVGTGVLIVPYRTAFSYEASEKVQVALVGV
                     GGRGTWFVETIPAMERVTALCDVNEAKIAETFTRWEELARKMADSQQPWERQRAKEYQ
                     RLCESKPKTYYDFRKMLDEASREIDAVIVATPDHTHAVISAAAMKADKHVFCEKPLTR
                     TVHESRALRAIARKSKVATSMGNQGTASGQFRRAVELVREGTLGEIREVRVWNDGGGA
                     DRREPPQGEQPIPPGFHWDLWLGPAAARPYHREWLQRNSWREFGTGQLGNWASHSANL
                     AFMALYVHELWLKESLPPEQRLIRIHADHSGINRLSFPRWERITWEIPPRGGMPAVKF
                     IWHNGQGSGGRELVEPLRQEAIGLVERDRELLQWAGALLIGTEGRIVLTGHNATFCLL
                     PHEKFKDVPKDRPLKLDASRGHEADWFAACRGGKPAWANFDYASALNEFLMLGNVATQ
                     VEDDLVYDPVEMKVVNNEKANELLSCEYRPGWTL"
     gene            1600..>2083
                     /locus_tag="PKI05_12945"
     CDS             1600..>2083
                     /locus_tag="PKI05_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53153.1"
                     /translation="MQATIRKFALRTIAAGMFLVANTWALMGVYSQVPPHQAAKIREA
                     ARQIKVRATPQKPRTVLIWNTPPHLMEKDPHKGYCIPYGEEAFRAIGEETGAFKPVVS
                     DDLAVFAPENIKQFDAIVLNNASGPWITPIGEDVKKPALAQLGSNPQEIEAVLRRSFL
                     D"
BASE COUNT          481 a          551 c          614 g          437 t
ORIGIN      
        1 ggataagccc taggaaggga cccacggagt ttctccctta gaaggtggat atccaggttg
       61 aactgaggac tatttcagtg gggaggtgtc gtatgtcgcg cgttttcaac cgccgccaat
      121 tcatcaaaag cgcagtggta ggcacagggg tgctcattgt cccctatcgt acggccttca
      181 gctacgaagc cagtgagaag gtacaggtcg ccctggtggg cgtcggcggc cgggggacct
      241 ggttcgttga gacaatcccc gcgatggagc gggtgacagc cctttgcgat gtcaacgagg
      301 caaagattgc ggagaccttc acacgatggg aggaactggc tcgcaagatg gccgattccc
      361 aacaaccatg ggaacgccaa cgtgccaaag aatatcaacg gctttgtgag tcgaaaccaa
      421 agacctatta cgacttccgc aaaatgctcg atgaggcaag ccgagagatc gacgccgtca
      481 ttgttgccac accggatcac acccatgcgg tgatctctgc cgcggccatg aaggcggaca
      541 aacacgtctt ttgcgagaag ccacttaccc gtaccgttca cgagtcgcgg gccttgcggg
      601 caattgcccg aaagtctaag gtggctacct ccatgggaaa tcaggggacg gccagcgggc
      661 agtttcggcg agcggtggag cttgttcgtg aaggaacgct gggggaaatc cgagaagtcc
      721 gtgtctggaa tgacggaggg ggagcggacc gccgggagcc accgcagggt gaacagccta
      781 ttccccccgg ttttcattgg gatttgtggt tgggtcccgc tgccgcgcgg ccatatcacc
      841 gcgagtggtt gcaacgcaat tcatggcgcg agttcgggac cggtcagttg ggaaattggg
      901 caagccatag cgccaacctg gcctttatgg ccctttatgt tcacgaacta tggctcaaag
      961 agtcactccc tcctgagcaa cggctcatcc gaattcatgc cgaccattcg ggcatcaatc
     1021 gcttgtcgtt tccccgttgg gaacgaatca cgtgggagat tccaccccgt ggcgggatgc
     1081 cggcagtaaa gttcatttgg cacaacggtc agggttccgg tggcagagag cttgtggaac
     1141 ccctccggca agaggcgatc ggcttggtcg aacgcgatag agagctcctc caatgggcgg
     1201 gcgccttgtt gatcggcact gagggccgca ttgtccttac tggtcacaac gcaacctttt
     1261 gcttgctccc tcacgaaaag tttaaagatg tacccaagga ccgacccttg aagctcgatg
     1321 cctcgcgcgg gcatgaagcc gactggtttg ccgcgtgccg aggtggaaaa ccagcttggg
     1381 ccaatttcga ctacgcctca gccttaaacg agttccttat gttgggaaat gttgccacgc
     1441 aagtagaaga tgatctcgtg tatgatcccg ttgagatgaa agtggtcaat aatgaaaaag
     1501 ccaacgagct tctctcttgc gaatatcggc cgggatggac cttgtgatct gttagatgtt
     1561 atccgtgttg tcttcggcaa aatagaaggg ataccaacca tgcaagcaac cataagaaaa
     1621 tttgctctac gcacgatcgc tgccgggatg tttttggtgg caaacacgtg ggcactgatg
     1681 ggcgtctatt cccaagtgcc tcctcaccaa gccgccaaga tccgcgaagc ggcccgccag
     1741 attaaggtgc gtgccacacc gcaaaagccg cggacagtgc ttatttggaa cacgccgccc
     1801 catctgatgg aaaaggatcc ccataagggg tattgcattc cgtatgggga ggaggcattt
     1861 cgggctatcg gagaagaaac aggggcgttt aagccggtcg tcagcgatga cctggccgtc
     1921 tttgccccgg agaacatcaa acagtttgat gccattgtgt tgaacaatgc atcggggccg
     1981 tggatcactc ccattggcga ggatgtcaaa aaacctgcct tagcccaact tggctcgaac
     2041 ccccaagaaa ttgaggccgt actgcgaagg agctttctcg act
//
LOCUS       DAOPTR010000763         2082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_41948_length_2082_cov_3.141095, whole genome shotgun sequence.
ACCESSION   DAOPTR010000763 DAOPTR010000000
VERSION     DAOPTR010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41948_length_2082_cov_3.141095"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="PKI05_12950"
     CDS             <1..1109
                     /locus_tag="PKI05_12950"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOA53154.1"
                     /translation="AKVIAIVTASMLGVLTGQVIGADLEHVVVFSAGEAGYHTMRIPA
                     IEVAGDGTILAFAEARKNSAADPGYPGQEIDLVMKTSRDGGRSWSDLVVLEHVGELWS
                     SANPAVVCDRTNGRIWLFYLQCAPGRSSHTARPGTLDVKNLVRFSEDNGRTWSEAIDL
                     TTVARDLSDKEWGISVPGPGGAIQDRQGRLIIPMWRYRPFRDFVLFSEDHGKTWHRGD
                     FVPTEEGLDESQLVELADGTLALDMRQQRGPHRWLSFSRDGGRTWPEPRPGQEVTPVC
                     CAVERWTLKSQGDDRDRVVWTGPRGPGRNNLTLWISYDELASFQNPRLIAEGPGAYSD
                     LTVLPDKSLGILWEQGNYKTITFTRVSLSWVEPR"
     gene            complement(1106..>2082)
                     /locus_tag="PKI05_12955"
     CDS             complement(1106..>2082)
                     /locus_tag="PKI05_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53155.1"
                     /translation="QQPMGRAFATSDDYIAHVKSVATAIRMIDPQAQVGVAIAISRPL
                     WGNYVLKHAAEYYDFVVAHYYGFENAYRSSFEELVLGQNYRILERIARCQALIDAYNP
                     GRHVCQLDTEWGMHSSGPEGERADYALRNANIIGVVHRAVRMIYYVREQPLAGASSWQ
                     MLGHSRGLGFTVLATDRPDSRTMLYWLYYYFLRHVGDNLVDFVGTAPFYPAIDHKTED
                     AATGPVTPVLLTTDTRQQCVFCIIANGSWSNDVPCRFLLKNYPGRLVSKVLLSDDDLD
                     RNPLLNTKDGFVRDIQMNVEVDRQSKSTIVTGTIPARSVVFLTLERLP"
BASE COUNT          476 a          543 c          592 g          471 t
ORIGIN      
        1 tcgcgaaggt tatcgcaatc gtcaccgcgt caatgctcgg tgttttaacc ggtcaagtga
       61 ttggggcaga cctagaacat gttgttgtgt tctccgcagg tgaagccggg tatcacacga
      121 tgaggattcc cgcaattgag gtggcaggtg acggaacaat cttggctttt gcggaagcta
      181 gaaagaacag cgccgctgat ccgggttatc ctgggcaaga gatcgatctt gtaatgaaaa
      241 cgagccgcga cggcggccgt tcttggtcgg atttggtcgt tttagagcac gttggtgaac
      301 tttggtcatc agcgaacccg gccgtcgtgt gcgaccgaac caacggccgg atttggctct
      361 tttacctcca gtgcgccccc ggaaggagta gtcatacggc gcgtcctgga actctcgacg
      421 tcaaaaacct agtgcgtttt agtgaggaca acggccgcac ttggtcagag gccattgact
      481 taacaactgt ggcacgggat ttgagtgaca aggaatgggg aattagtgtg ccagggccag
      541 gcggtgcaat ccaggatcgc caggggcgac taattatccc catgtggcga tatcggccct
      601 ttcgagactt tgtccttttc agtgaagacc acggtaagac atggcatcgc ggtgattttg
      661 tgccgaccga agaggggctc gatgaatccc agctcgtgga gttagccgac ggcaccctcg
      721 ctttggacat gcgtcaacag cgtggtcccc accgctggct aagctttagc cgtgacggcg
      781 ggcgaacttg gcccgaacca cgaccgggtc aagaggtaac acccgtttgc tgcgctgtcg
      841 agcggtggac cctcaagagc caaggggacg atcgggaccg cgttgtctgg accggtcccc
      901 gtggccctgg acgcaacaat ctcacacttt ggatcagtta cgatgagttg gcctcttttc
      961 agaacccccg tttgatcgcc gagggacctg gggcgtactc cgatctgacg gtgcttcccg
     1021 ataagagttt gggaatcctg tgggagcagg gtaactacaa gacaattacc tttacccgtg
     1081 tgagcctttc ctgggtcgaa ccgcgttaag gaagtctctc cagggtcagg aagacgacac
     1141 tacgggcggg aattgtcccg gtcactatgg tacttttgct ttgcctatcg acttccacgt
     1201 tcatttggat atcacgtacg aaaccatctt ttgtatttag caaagggtta cgatccaagt
     1261 cgtcatcgga gagaaggact tttgagacca gtcttcccgg atagttcttg agaaggaaac
     1321 gacacggcac atcattcgac catgatccat tggcgataat gcaaaatacg cattgctgcc
     1381 gtgtatcagt tgtcagcaag acgggcgtaa caggacctgt cgctgcgtct tcggtcttgt
     1441 ggtcaatcgc cgggtaaaaa ggggcagttc ccacaaaatc aacaagattg tctccaacat
     1501 gtcgcaggaa gtaatagtag agccaatata acatcgtccg cgaatctggt cggtccgtcg
     1561 ccaacaccgt gaaacctaac ccccgtgaat ggccaagcat ttgccacgag gacgcaccgg
     1621 caagcggctg ttcgcggacg tagtaaatca tgcggactgc acggtggact accccgatga
     1681 tattcgcatt gcggagggca tagtccgcgc gttcaccttc cgggccggat gagtgcatac
     1741 cccattcggt atcgagctgg cacacgtgcc gccctggatt gtacgcatct atcagggcct
     1801 gacaacgagc gattcgctcg agaatacggt agttttggcc caggacaagc tcttcgaacg
     1861 agctacggta ggcattttca aaaccgtagt aatgggccac gacaaaatcg taatattctg
     1921 ctgcatgttt tagcacgtaa ttaccccaca acggtcgcga tatcgcaata gcaacaccga
     1981 cttgggcttg gggatctatc attcggatgg ccgttgccac ggacttcaca tgggcgatgt
     2041 aatcgtcaga cgtagcgaac gccctaccca tgggctgctg cg
//
LOCUS       DAOPTR010000764         2077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_42090_length_2077_cov_3.056380, whole genome shotgun sequence.
ACCESSION   DAOPTR010000764 DAOPTR010000000
VERSION     DAOPTR010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42090_length_2077_cov_3.056380"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PKI05_12960"
     CDS             complement(<1..564)
                     /locus_tag="PKI05_12960"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53156.1"
                     /translation="MSLISNIWQWSTVLPPQRLRRIGWSSNIWSITVVTVFAFGAVLT
                     ANANEGSAMKVESQVFGTMPSGEVIPLYTLDNGRGLVVRVMSLGATLTAVEFPDREGQ
                     RENVTLYLESLEDYLKGHPCFGSICGRYANRIAKGQFQLDGVTYQLATNNGPNHLHGG
                     KVGFHQRVWDHRPVQGDGWVGVEFSRVS"
     gene            complement(657..1868)
                     /locus_tag="PKI05_12965"
     CDS             complement(657..1868)
                     /locus_tag="PKI05_12965"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HOA53157.1"
                     /translation="MKILVVNAGSSSVKFGLFDWETLVPTVRGLVDFNAQPDGAQIIV
                     REGDTVVAQRTSPSKKYRDAVAGALSLLQELHFVASPEEIPAVAHRLIHGGEAIREPV
                     IITQQIKEIIRQFIPLAPLHIPAGLQAIEVTESLFPGAVQTGIFDTAFFAHIPPAAYL
                     YPVPYEWYTEWGIRRFGYHGTSHAYAMERAAQMLGRPVESVHLITCHLGQGSSATAIA
                     EGKAVTNTMGFTPLEGFMMGTRSGTVDPGIITYVQRHHGLTADQVERILHTQSGLLGV
                     SGISGDYREVLRAAQAGHKRSQLALEMYAYRVRAMLGALAVTLGKVDALVFTGGIAEN
                     ASELRADICRGLECLGLFLNPERNAACQPDADISSDNSPGRILVIRSQEDYILAREAK
                     RLVSANRAGFA"
BASE COUNT          523 a          596 c          542 g          416 t
ORIGIN      
        1 actgacgcgg gagaattcga ccccaaccca gccgtcaccc tgcaccggcc gatggtccca
       61 gacccgctga tggaatccga ccttgccacc atgcagatgg ttggggccgt tgtttgttgc
      121 caactggtaa gtcacaccat ccaactgaaa ttgccctttg gcaattcggt tcgcataacg
      181 cccacaaatg gaaccaaaac acggatggcc cttaaggtaa tcctcgaggg actctagata
      241 aagggtgacg ttctcgcgtt gtccctctcg gtcgggaaac tctacagccg tcagcgtagc
      301 acccaaagac atgacacgca caaccaaccc ccggccgttg tccaaagtgt agagaggtat
      361 gacttcgcca ctgggcatgg ttccaaaaac ttgactctcg actttcatag cacttccctc
      421 atttgcatta gcggtaagaa ccgcgccaaa agcgaacacc gttaccacag tgatcgacca
      481 tatattggag gaccagccta tccgacgtaa ccgctggggt ggcaaaaccg tcgaccattg
      541 ccaaatattg gaaatcaaag acatgactca acctcccttc ccgtgctccg gtttaaatcc
      601 cgcgctctga cgaccaatgt tgggttgggc accaagtttg gttacaaggc taccggtcat
      661 gcaaaacctg cgcgattcgc actgaccaaa cgtttggcct ccctcgccag aatataatcc
      721 tcttgactgc gaataactag aatgcgaccg ggagaattgt cggaggaaat gtcagcatct
      781 ggttggcacg cagcatttcg ctcggggttc aaaaatagtc caaggcactc cagaccgcgg
      841 cagatgtcgg cccggagttc cgaggcattt tcagcaattc caccagtaaa aacgagggca
      901 tcgacctttc ccaacgttac ggccaaagca ccgagcattg cccgcacgcg gtaagcatac
      961 atttccaacg ctagttgaga ccgtttgtga ccggcctggg cggcccgaag gacttcgcgg
     1021 taatcccccg aaatgcctga cacaccgagg aggcccgact gtgtatgcaa aatccgttcg
     1081 acctggtcag cagtcaggcc atggtggcgt tgaacatagg tgatgatgcc cgggtccacg
     1141 gttcctgaac gcgtccccat cataaagcct tccagcgggg taaaacccat ggtgttggta
     1201 acagccttac cctctgcaat cgcagtggcc gaagaacctt gcccaaggtg gcacgtgatg
     1261 agatgcaccg actccaccgg gcggccgagc atttgggcgg cacgttccat ggcgtaagcg
     1321 tggctcgttc catgatagcc gaaacgacga atgccccact cggtgtacca ttcgtaggga
     1381 acggggtaaa ggtaagctgc cggcggaatg tgggcaaaga aagccgtgtc gaaaattccg
     1441 gtttggaccg cgccgggaaa tagagactcg gtgacctcga tagcctgaag cccagccgga
     1501 atgtgaagcg gcgcaagcgg gatgaattgg cgaataatct ctttaatctg ctgggtgata
     1561 atgaccggtt cgcggatagc ttcgccccca tgaatcaggc gatgcgcaac ggccggaatc
     1621 tcttcaggtg aagccacaaa gtgcaactcc tggaggagac tgagggctcc cgccaccgca
     1681 tcacggtact ttttagaggg cgacgtacgc tgcgccacga cggtatcgcc ttctcgaact
     1741 attatctggg caccatcggg ctgggcgttg aagtccacca atccccgcac ggtaggaaca
     1801 agcgtctccc aatcgaaaag gccaaatttg acgctgcttg aaccagcgtt tacgaccaaa
     1861 attttcatgg aatgaaaccg gaaatctcag tcttgggcca ccattaagac caccctgcta
     1921 ttgtgacaaa gccttaacag cttgcttgag tgcctcccga gcagatcggc tgtattccac
     1981 gtcaagccaa acgattctcc cggaaccgtc caaaagatac aaacagggtg gctgatgagc
     2041 cgaaaattgg cggaaaaacc caccatcggt gtcaacc
//
LOCUS       DAOPTR010000765         2075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_42144_length_2075_cov_3.304950, whole genome shotgun sequence.
ACCESSION   DAOPTR010000765 DAOPTR010000000
VERSION     DAOPTR010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42144_length_2075_cov_3.304950"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PKI05_12970"
     CDS             <1..653
                     /locus_tag="PKI05_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53158.1"
                     /translation="LERTREVRRPEGPPPEVRASMMERIERAMNETREKLREAREAGR
                     EEAVRDLERRLEQLERTMRDLREGRRPELPAGLGLETPPAVARERAMERLERAMNEVR
                     ERLKKAREEVREGEVRELEQQLARMEGQMAALREGRPLLPPGAPVPERIERARRAAEL
                     LRREGFGDMAELLMRSLEREMRPLPPREPGRVGPPDMPLPPPARRPDRPDAPRPER"
     gene            complement(924..1445)
                     /locus_tag="PKI05_12975"
     CDS             complement(924..1445)
                     /locus_tag="PKI05_12975"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HOA53159.1"
                     /translation="MVKSKRNALNRSGVLTLEAVLVLSILILVTLAVFQFAITMLIEQ
                     TITHAVTVAAREAGKGADIDTTVESVNAVLQLHGLAIGPGATLILEDPLAIPPVSARG
                     SLDCQPPTQPIVSPGYIRTTLCVSLGQRPFLKLLKTFCLDFSGKIFTVSAVARREFTG
                     PPEVTSRPHCNCQ"
     gene            complement(1541..>2075)
                     /locus_tag="PKI05_12980"
     CDS             complement(1541..>2075)
                     /locus_tag="PKI05_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53160.1"
                     /translation="YSRTLTIDFFPDPDTGDLGFEPGDRIRFGARTTGVSKGSGQDDG
                     DGIGRDGVTVTVVFAINGVPLPAVTRTFIDNEETSNDCQPTCPVHPSGIDDLPCPPAS
                     SPNNNGQSYVILGGGSGGDYAVRAQAVVAVPSPICKLFGLRMPRYRVSAKTIAIHSCE
                     EQRTRLQRVDQYICVAP"
BASE COUNT          497 a          537 c          612 g          429 t
ORIGIN      
        1 agcttgagcg aacgagagag gtgcgtcgtc cggaaggccc accgcctgaa gtccgagcat
       61 cgatgatgga acgaatcgaa agggccatga acgaaaccag ggaaaaattg cgagaggcca
      121 gagaagcggg acgcgaagaa gctgttcgcg atctggagcg tcgtctcgaa cagttggagc
      181 gcaccatgcg cgatctgcgt gaaggacgcc gccccgagtt gccagcgggt ctggggctcg
      241 aaactccgcc tgcggttgcg cgggaacggg cgatggagcg actggagcga gccatgaacg
      301 aagtccgcga gcgactcaag aaggctcggg aagaagtccg cgaaggtgaa gttcgtgaac
      361 tcgagcaaca gttggctaga atggaagggc aaatggccgc actgcgagag ggtcggccac
      421 tcctgcctcc tggagcacct gttcccgaac gtatcgagcg agcacgccgc gcagcagaat
      481 tgttacgacg ggaaggtttt ggggatatgg ctgaattgtt gatgcgctcc ttagaacgcg
      541 aaatgcggcc attacctcct cgtgaacccg gtcgggtagg tcctccggat atgccgctgc
      601 ctccacctgc tcggcggcct gatcgaccag atgctccgcg accagagcgg tgatggacgg
      661 tcgctgaacc tttaggagag tgaggttgtt tgtctcccac tcagtgtgaa gcaatgcgaa
      721 agtatgacct ggggtgcagt atgactactg caccccattt gttttcgcta ggcttgaagg
      781 ccaatccata atctcccttc acctgttcgg cgggagaggg ggcttttggt gataggctgt
      841 tatttggaaa gaatcggcta aatatacctc ttacggaacg cgcgttgtgc tctattatcg
      901 gcgaagtgaa aacccatcgg agattactga caattgcagt ggggacgaga ggtgacttct
      961 ggtggaccgg tgaactcccg gcgtgcaacc gccgacaccg tgaatatctt accggaaaag
     1021 tccaagcaaa atgtcttcag caacttcaag aaagggcgct gtccgagact tacacagagc
     1081 gtagtgcgaa tataaccggg agagactatc ggttgtgtcg gcggctggca gtccaagcta
     1141 ccccgagcac tgacgggagg tatcgcaaga ggatcttcaa ggatcagagt agcccctgga
     1201 ccaatagcaa gcccatggag ttgcagcacc gcgttgaccg attccacggt ggtgtcaata
     1261 tcggcccctt ttcctgcttc gcgagctgcc accgttacgg cgtgcgtgat agtctgctca
     1321 ataagcatgg tgatcgcgaa ctgaaaaacg gcgagcgtca ccaggatgag gatagaaagc
     1381 accagaacgg cttctagtgt cagaacgccg ctacggttta acgcatttcg ttttgactta
     1441 accatggttt ggctctctcg ggcactattt caaagctttg cgaagcctct cgaccgcact
     1501 cccacagaga actgggtgag ctatccttgg tgagctcgct ttatggcgca acgcagatat
     1561 actgatcgac cctctgcaaa cgtgtccgct gctcttcgca ggagtgaatg gcaatggtct
     1621 tagcggacac tcggtatcgt ggcatccgca aaccaaaaag tttgcaaata ggactgggaa
     1681 ctgccaccac ggcttgggct cgcacagcat agtccccgcc agatccgccg ccaagaataa
     1741 cgtacgactg cccattgtta ttgggggaac tagcgggtgg acaaggcaaa tcgtcaatac
     1801 ccgatggatg cacgggacat gtgggctgac aatcgttcga tgtttcttcg ttgtcgataa
     1861 atgtccgagt tacggctggc aaaggaacgc cgttaatcgc aaagacgaca gtgaccgtta
     1921 caccatcgcg gccaatgccg tctccatcgt cctgcccact tcctttagac acaccggtgg
     1981 ttcgtgcacc aaaacgtatg cgatcgcctg gttcaaatcc taggtcacct gtgtcaggat
     2041 cggggaaaaa atcaattgtc agagtacgtg agtag
//
LOCUS       DAOPTR010000766         2064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_42463_length_2064_cov_3.555998, whole genome shotgun sequence.
ACCESSION   DAOPTR010000766 DAOPTR010000000
VERSION     DAOPTR010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42463_length_2064_cov_3.555998"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1697)
                     /locus_tag="PKI05_12985"
     CDS             complement(<1..1697)
                     /locus_tag="PKI05_12985"
                     /inference="COORDINATES: protein motif:HMM:NF019318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOA53161.1"
                     /translation="MAWSRLRRTEVALRFVIGLVAATVLCFLIQAWNPPPLLRLDAEL
                     ARDSMVGLPAGTLQWHRPLLQDNEPPPQEGSETATPRAWSKLVLRFLGVWTLVVFLFG
                     VGGLYIFFRERRVLVSLNRLLTLLGLVILSVVIARWLAADIPYTECIPLLLLAQTLAV
                     AYHREFALTLGGIVLVLFLLVTGYEVGMGILWASVLSVAILQLDSVRTRTKLLTVSAV
                     AASVGMVVRFLLALNNDVPLDMALLGELWRIALACIVSGFLMHGLLPFIERFFGILTD
                     MSLLELSDLSHPLLRELARRAPSTYNHSINVGAIAEAAADSIGARGLLVRVGAYFHDI
                     GKMLKPNYFIENQREAENRHETLEPTISSLIIIAHVKDGADLARQHHLPEPIIDFIEQ
                     HHGTTLVEYFYERAKEKREGQAENNKVDENNFRYPGPAPQTKEAAVLMLADAVESAVR
                     SLKDPAPSRIEAVVRDIAERKLHEGQFDETNLTLRELRTIEDSLIRSLVAMYHGRIKY
                     PERRTEERRETERRTESSGGAAVGVTKLAGSPKNGQSTPNSEKAVPGESANAVPAGK"
     gene            complement(1777..>2064)
                     /locus_tag="PKI05_12990"
     CDS             complement(1777..>2064)
                     /locus_tag="PKI05_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOA53162.1"
                     /translation="DTTQTDLPAHTRSGLVDAWTRLRQIRGIAQVELTTADIVRHPLV
                     QAIVDAYEQHPSKRDPKARETTQESLPANQSDTANDASTSPGEVLNKETTH"
BASE COUNT          434 a          591 c          615 g          424 t
ORIGIN      
        1 tctttgcccg cggggacggc gttagcagat tcgccgggaa cagctttctc gctatttgga
       61 gtggactgcc cgtttttggg cgatcctgcc agtttcgtca cccccaccgc cgcaccgccg
      121 gacgattcag tccgccgttc tgtttcgcga cgctcctcgg ttcgccgctc tgggtatttg
      181 atgcgcccgt ggtacatggc caccaacgag cggatgagac tatcctcgat ggtccgcaac
      241 tcgcggagcg tgaggttggt ttcgtcgaac tggccttcgt ggagctttcg ctcggcaata
      301 tcgcggacga cggcctcaat ccgcgaaggg gccgggtcct tgaggcttcg cacggcactt
      361 tccacggcgt cggccaacat gaggacggcg gcctccttgg tctgaggtgc tggcccagga
      421 tagcggaaat tgttttcatc gaccttgttg ttttccgcct gtccctcccg tttttccttt
      481 gccctttcat aaaaatactc gacgagtgtc gtcccgtgat gctgttcaat aaagtcaatg
      541 atgggctcgg gcagatgatg ctgccgggcc agatcggccc catctttgac gtgggcgata
      601 ataatcaggc tgctgatcgt cggctcgagg gtctcgtggc ggttttcggc ttccctttgg
      661 ttttcaatga aataattggg tttaagcatc ttaccgatat cgtggaaata cgcccccaca
      721 cggacgagca agccccgggc gccgatagag tcagccgccg cctcggcaat cgctccgacg
      781 ttaatcgagt gattataggt gctgggggca cgtcgtgcca gttcgcggag cagcggatgc
      841 gagagatcgc tcagttccaa aaggctcatg tcggtgagaa tgccgaagaa ccgctcgatg
      901 aacggaagca gtccgtgcat gagaaaaccc gacacaatgc acgccagggc aatccgccac
      961 aattcaccca agagggccat atcgagcggg acatcattgt tcagcgcgag caggaaacgg
     1021 acgaccatcc ctacgctggc cgcgactgca ctgacggtga ggagtttcgt gcgtgttcgg
     1081 accgaatcga gttgaaggat ggctacagaa aggacgctgg cccaaagaat gcccataccc
     1141 acttcgtagc cggtgacaag cagaaatagc accaagacga tgccaccgag cgttagggcg
     1201 aattcgcgat gataagcgac ggccaaggtc tgggccaaaa gcaaaagggg gatgcactcg
     1261 gtgtagggaa tgtcggcggc cagccaacgg gcaatcacaa cactgagaat cacaaggccg
     1321 agaagggtga gcagtcggtt gaggctaaca aggacccgcc gttcgcggaa aaagatatac
     1381 aaccctccca ccccgaataa aaacaccacc aaggtccaca cgcccagaaa tcgcaggacc
     1441 aatttggacc aggcccgggg cgtagccgtt tctgatcctt cctggggagg tggctcattg
     1501 tcctggagca aagggcgatg ccactgaagc gtgccggcgg ggagaccgac catgctgtcg
     1561 cgcgcgagtt cggcatcgag ccggagcagg ggtggtggat tccacgcctg tatcaagaaa
     1621 cacagcactg tggcagccac caggccgatg acgaaccgca gggccacttc ggtgcggcgg
     1681 aggcggctcc atgccacttc ccactttccc ggtaccctta ccaggccggc cagtctctcg
     1741 ctccgcttgc gttttgttcc tccaaaaaac atgaggttaa tgagtcgtct ccttgtttaa
     1801 gacctcaccg ggcgaagtcg acgcatcatt ggcggtatcg ctttggttcg ccggtaggga
     1861 ttcctgtgtc gtctcccggg ccttggggtc ccgttttgac ggatgttgct cgtaggcatc
     1921 gacgatcgct tggaccaaag gatgccgaac aatatccgcc gttgtcaact cgacctgggc
     1981 aattccacga atctgacgca ggcgggtcca ggcgtcaacc agaccactgc gagtatgggc
     2041 cggaagatcg gtctgtgtcg tatc
//
LOCUS       DAOPTR010000767         2064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_42466_length_2064_cov_3.352912, whole genome shotgun sequence.
ACCESSION   DAOPTR010000767 DAOPTR010000000
VERSION     DAOPTR010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42466_length_2064_cov_3.352912"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..1515)
                     /locus_tag="PKI05_12995"
     CDS             complement(199..1515)
                     /locus_tag="PKI05_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA53163.1"
                     /translation="MDRQRISTRRHFLKVTAGLSAGALMPMIVPRHVLGTETTPSANE
                     RIGIGGIGIGRRGLQLFEEIPSLGRVVAIADVNLPRAEKTAEPYRAVAYQDYRKLLER
                     KDVDAIITATPDHWRAIVCIHACQAGKDIYAEKPMSLTIYEGRKMVEAVRKYDRVFQT
                     GSQQRSMAANRLGCELVRNGRIGKVHTVIAANYPSPWECALPGQPVPAGLDWDMWCGP
                     TEPVPYHIDLYTPRANPGWISFRPYSGGEMTGWGSHGLDQIQWALGTDDTGPVEIWTE
                     GPKFSPPTYTQPESRARGDKICSQPKVFFKYASGVVVKFDNGPPGGAIFIGDKSRITV
                     DRAKFTVEPKELGDPPYDDFSVRLYVSNNHMLNWLECIKTRQKPVADVEIGHRSTTLC
                     HLGNIARWVGRPLKWDPVKEQFIGDDEANSYLDRPRRKPYVLPDPV"
     gene            complement(1659..>2064)
                     /locus_tag="PKI05_13000"
     CDS             complement(1659..>2064)
                     /locus_tag="PKI05_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOA53164.1"
                     /translation="IASSAHRYGIYWYEQVREGGTIRFRRHLIDNTWSQAHSLTLADL
                     DGDGDLDLVTGKRFMAHNGSDPGEFDPLGVYWYEFEAKPQPTWIKHAITYDQGIGAGL
                     NIPVVDLDGDGDLDIVVTGKWGGPVWFENKLK"
BASE COUNT          456 a          627 c          510 g          471 t
ORIGIN      
        1 ttcttgccct tcccacctgg ctggcattgg tcatcccggc agcgttggct gtggcggtgt
       61 tccgaccgcg ggccctctta ctcctacttc ctattataat tgtcatcgcc atcctgcgac
      121 cccgcaaaag acccaccaac ggaagtcgca aaaaagcgcg ctaggccatg tgcaagacat
      181 caaaaagccg ccgagatttc acaccggatc cggcaagaca taaggcttcc gtcgcggacg
      241 gtcaaggtac gaattggctt catcatcgcc aataaactgc tccttcaccg ggtcccactt
      301 caaaggtcgc cccacccatc gggcgatatt acccagatga cacagcgttg tcgaacggtg
      361 tccgatttcc acatcggcca caggtttctg ccgggtcttg atgcactcca gccagttcaa
      421 catgtgattg ttgctgacat aaagtcgaac agaaaagtcg tcataggggg ggtcacccaa
      481 ctctttcggc tcaaccgtga atttggcacg gtcaacggtg attcggctct tatcaccgat
      541 gaaaatcgcc ccgcccggcg gtccgttgtc aaatttgacg acaacgccac tcgcgtactt
      601 aaaaaagact ttcggctgac tgcagatctt atcgccccgg gcacgggact ccggctgggt
      661 atatgttggc ggagagaatt tcgggccctc tgtccaaatc tcaaccgggc ctgtgtcatc
      721 ggtacccaag gcccactgaa tctgatcgag accgtgggac ccccaacctg tcatttctcc
      781 gcctgagtac ggccggaacg aaatccatcc cggattggcc cggggcgtgt acaggtcaat
      841 gtgatacgga accggctctg ttgggccaca ccacatatcc caatccaatc ccgctggaac
      901 tggctgaccc ggcaacgcgc attcccaagg actaggatag ttggccgcaa tcaccgtgtg
      961 gaccttgcca attcgaccgt ttcgcaccaa ttcgcacccg aggcggttgg ccgccataga
     1021 gcgctgctgg ctgccggttt gaaacactcg gtcatatttc cgaacggcct cgaccatttt
     1081 tcgcccctca taaattgtga ggctcatcgg tttttcggcg taaatatcct tgcccgcttg
     1141 acatgcatga atacagacaa tggcccgcca gtgatcgggc gtggccgtga tgatggcatc
     1201 gacatccttg cgttcgagaa gctttcggta gtcttggtat gcaaccgcgc ggtatggctc
     1261 ggccgtcttc tccgcccgtg gaaggttgac atccgcaatg gcaacaactc gaccaagcga
     1321 aggaatctcc tcaaacagtt gcaggccccg acgaccaatg ccgattcccc caatgccaat
     1381 cctttcattc gccgatggtg tggtttcagt tcccaggacg tgccgcggca caatcatcgg
     1441 catgagtgca ccggcggaaa gtccggctgt aaccttcagg aagtggcgtc gagtactaat
     1501 tctttgccta tccattgaca atctccccgt cataaaattg agccgatcga aatgatgtca
     1561 gccggcgatt atttcacgga attatctgct atgagcgaac ccactcgcga aaataagcca
     1621 catcttcagg cacttgtcaa gtcggttaat agcgtgccct atttgagttt gttttcaaac
     1681 cacaccgggc caccccattt gcccgtgacc acaatatcca ggtctccgtc gccatccaaa
     1741 tccactaccg gaatgttcaa acccgccccg attccttgat catacgtgat ggcatgtttg
     1801 atccatgtgg gctggggttt ggcctcgaac tcgtaccagt aaactcccaa gggatcgaac
     1861 tccccgggat cgctcccgtt gtgtgccatg aaccgctttc ctgtcaccaa atccaagtcg
     1921 ccatcaccgt cgaggtcagc cagtgtcagc gagtgggcct gactccaggt gttgtcgatg
     1981 agatgtcgtc gaaaacgaat cgtcccacct tcgcgaacct gctcgtacca gtagattcca
     2041 taccggtgtg ccgaactggc gatt
//
LOCUS       DAOPTR010000768         2062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_42533_length_2062_cov_2.786248, whole genome shotgun sequence.
ACCESSION   DAOPTR010000768 DAOPTR010000000
VERSION     DAOPTR010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42533_length_2062_cov_2.786248"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PKI05_13005"
     CDS             complement(<1..441)
                     /locus_tag="PKI05_13005"
                     /inference="COORDINATES: protein motif:HMM:NF019266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53165.1"
                     /translation="MRTVWTVPLMAMLMIAVAAGKVDAGYCGAVRLALQPTGCALEPG
                     CGPQQCYTVLKPSRKLVFEPQQVTSYRTHFERVVEPRQVTYTRYVPEIQYREVARTVM
                     KPVWETRTHQLAYTVMKPVWETRTKEIPYTVMKPVWETRTRTYTV"
     gene            994..1452
                     /locus_tag="PKI05_13010"
     CDS             994..1452
                     /locus_tag="PKI05_13010"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOA53166.1"
                     /translation="MNRGVRRAFTLIEVLIVVVIMAILAAVIIPQFAASTSDAKRSAL
                     EFNMHTLRSQIELYRIQHGQYPTISNNDLPQLYNATNLAGEIGTPGPNYPYGPYLANG
                     LPPNPYNNSNKVAPVSTPGQKPTGVVSGDYGWQYDQSTGAIWPNNPEYYQ"
     gene            1721..>2062
                     /locus_tag="PKI05_13015"
     CDS             1721..>2062
                     /locus_tag="PKI05_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53167.1"
                     /translation="MSEEPDRPRVLEREEYIEQAYFFRALRERLDQGVSTQELIKTIA
                     QEILSTTKLPLALDFMVTELRFTGGFSTAMARLGHYFTAFQTYVIREAEREDGRLDFR
                     IALEILEKEAEY"
BASE COUNT          495 a          604 c          503 g          460 t
ORIGIN      
        1 caccgtgtat gtccgtgtcc gcgtctccca taccggcttc atcaccgtat agggaatctc
       61 cttcgttcgc gtctcccaca caggcttcat caccgtgtag gccaattggt gtgtgcgggt
      121 ttcccaaacc ggtttcatga cggtccgggc aacctcccgg tattggattt ccgggacgta
      181 cctggtgtat gtcacttggc ggggctcgac cacacgctcg aaatgcgtcc tgtagctagt
      241 aacctgctgt ggttcgaata caagtttgcg gctaggtttc agaaccgtgt agcactgttg
      301 cggcccacaa cccggctcca gcgcgcagcc agtcggttgc agggcaagtc gcacagcccc
      361 acagtaaccg gcgtcgactt tccccgccgc aacggcgatc atcagcatcg ccataagcgg
      421 cacggtccag acggttctca tgttatccct ccttttgatg tagcaaatca aaaatgggcc
      481 tggtggggac gcggcagttt ccacctattg tgaggtttcg tccaaattca aacggcagcg
      541 ttagctgatc ggcggctggt gcataattca cattgcccac tcaactcata ccggccgcac
      601 aaccgctacc gcttcccaaa attactcccg tgcttaccca actgaaaata tcggcaattt
      661 ggagagataa gttaaccagc gaaatatggg gccacaagaa acgacgatat aaactatgga
      721 aaatacaaaa cttgcgggca tctttgacga ttagcgcgaa gtcacagccg gactttccgc
      781 cccaaccaga cccttacggg caactcatcc cgctaaatgc cccgaatctc gcaatgaaag
      841 atgttcggtt gccgtaaact cctcgaaaag atttgccact agcttgttcg caacctacat
      901 tgcagtagaa aagttctctg ccccgggcga gcagaccagg aagaaacgaa ccattaacta
      961 catgacacaa tttcagacag aggagaccaa tttatgaatc gaggtgttcg tcgggcgttc
     1021 acgctgattg aagttctgat cgttgtggtg atcatggcca tcctcgccgc cgtgatcatc
     1081 ccacagttcg ccgcatccac gtcagatgca aagcgcagtg ccctggaatt caatatgcac
     1141 acgctacgat cgcaaattga gttatatcgc atccagcacg gccagtatcc tacgatcagc
     1201 aacaatgacc tccctcagct ttataacgca accaatctcg ctggcgagat cggcacgccc
     1261 ggcccgaact atccatacgg gccatacctc gccaacgggt tacctcccaa cccctacaac
     1321 aacagtaaca aggtggctcc ggtcagtacc ccgggtcaaa aaccgaccgg ggttgtctct
     1381 ggggattacg ggtggcagta cgaccaatct acaggcgcca tttggcctaa taacccagaa
     1441 tactatcaat agggctcttt gaagctcacc agccccaata aactcagcga cgtcacgtta
     1501 tatctcaaac caccagactt cccggtggtc ttttcttttg ggcgctccgc agccccgcag
     1561 cattaaaggg ctccgagcat tcacgccgcg tggcgctcac caggggcatg ttccttgaaa
     1621 ccgcccaggc ttagaatggt aatcattcag taagcccaag cgcgtgtttg ttcttgacta
     1681 ctgtcgttcg aacctcccca gtcggcggga aagagttgcg atgtcagagg aacccgatcg
     1741 gcccagagtt cttgagcgcg aagaatacat cgagcaggcc tatttctttc gggccctccg
     1801 ggagcggctt gatcaagggg tttcaactca agagttgatt aaaacgattg ctcaggagat
     1861 tctctccaca accaaactcc ctctggcctt ggactttatg gtcaccgagt tgcggttcac
     1921 cgggggtttt tcgacggcaa tggccaggct aggccattat ttcacagcct tccaaacgta
     1981 tgtcatccgg gaggccgaac gggaggatgg ccgcctcgat ttccgtatcg cccttgaaat
     2041 tctggaaaaa gaggccgagt ac
//
LOCUS       DAOPTR010000769         2061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_42550_length_2061_cov_3.245763, whole genome shotgun sequence.
ACCESSION   DAOPTR010000769 DAOPTR010000000
VERSION     DAOPTR010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42550_length_2061_cov_3.245763"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2061)
                     /locus_tag="PKI05_13020"
     CDS             complement(<1..>2061)
                     /locus_tag="PKI05_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53168.1"
                     /translation="MPQIFGLTDHLSEGSAQYRKQFEQVEIAGIVAYLVGQSRPLPSH
                     LAETRAPSQDKLGSRSLADAGRILFATQGCLACHRHRDFPEGTSDVGPDLSDLGQRLA
                     SDTGREWLRLWLTSPQTLSPSTRMPQPQFRDDAWIYRVFEPAESKPRRVTSSEVIDAL
                     VAFLLSGESSTNGSQQPEKLPVNAEWLDEVVLEYLSSDLPREKAQGIVRNGATVADLG
                     KYSLQVALELTELLGEPTSDKKLRYVGRKAIGRYGCYGCHDISGFEGWPPIGPSLSQF
                     GSKPVALLDFGAVTEGLFSTPEGRRILQRRKTPLEDRLSSLDFLGRETWLWLKLISPR
                     AFDFKAMEGKPILQHSRMGRFPLSGEERQAIMAFILGLEGRPVPPKYDPYSASRKALA
                     LGREIIERRACDRCHALRPEQWFFRFSEEDVVREMGGDYDPIGRQTEAEKASAMPPRR
                     PLGYALAMGQAERDASGQIIEDRDDFDNPLYFFMPWRPVAFGAHIWPVGEASIPIAST
                     QLLGKRDQDGGAFAFMLYPTVVSLIQQIQLSMGLKETMGCLPPPLYYEGVMVQPSWLS
                     QYLKEPYPIRPAIPLAMPRYWLTNEEVQILVDYFSALAEKTGESDTSWKTTETQLILR
                     EQQWPGRLDAAWHLIHDTKTYCAKCHISSDNMEDRLALPTEAPRLDGVYRRLQHDFIR
                     AWVV"
BASE COUNT          482 a          594 c          542 g          443 t
ORIGIN      
        1 ggactaccca tgcccggata aagtcgtgtt gcaagcgtcg ataaactcca tcaagtcgcg
       61 gggcctctgt cggcagggcc aggcgatctt ccatattgtc cgaagaaata tgacatttcg
      121 cacaatacgt tttcgtatca tggatcaagt gccaggccgc atcgagccgc cctggccact
      181 gctgctcacg aagaatcaat tgagtttccg tcgttttcca acttgtatcc gactcaccgg
      241 tcttttccgc cagcgcgcta aaatagtcaa caaggatttg cacctcctca ttcgtcaacc
      301 aatagcgtgg catagccaac ggaatggccg gtcggatggg ataaggttcc ttcaaatact
      361 gagataacca cgaaggctga accataacgc cttcgtaata aagcggaggc ggcaggcatc
      421 ccatcgtttc ttttagtccc atggacaatt ggatttgttg gatgagagag accaccgtgg
      481 gatataacat gaaggcaaac gcacctccat cctggtctcg cttccctagt aattgcgtcg
      541 aggcaatagg gatactcgct tcacccaccg gccaaatgtg agcaccaaag gcaactggcc
      601 gccagggcat gaaaaaatac agtggattgt caaaatcgtc acggtcttcg atgatttgcc
      661 cggaggcgtc acgctcggct tggcccatag ctagggcgta gcccaggggg cgccgcggtg
      721 gcatggcact ggctttttcg gcttctgttt gccttccaat tgggtcataa tccccgccca
      781 tttcccgcac tacatcttct tccgaaaatc gaaagaacca ttgctccgga cggagggcat
      841 ggcaccgatc gcacgcccgg cgttcgatga tctcacgacc caaggcgagt gcttttcggg
      901 acgccgaata agggtcatac ttcgggggca ctggtcgccc ttcaagtcct agaataaacg
      961 ccatgatcgc ctggcgttcc tcgccggaaa gtggaaatcg acccatccgc gaatgttgca
     1021 ggatcggctt gccttccatg gccttaaaat caaatgcccg aggagaaatg agcttcagcc
     1081 acaaccacgt ttcgcgaccc aggaagtcga gagaagagag gcgatcttcc aagggtgttt
     1141 ttcgcctttg taggatgcgc cgtccttctg gcgtggagaa caacccttcc gtcactgccc
     1201 caaagtccaa gagcgccact ggcttggatc cgaactgact caaactcggt ccgatgggtg
     1261 gccaaccctc aaaaccggaa atatcgtgac agccgtagca tccatatcga ccgatggcct
     1321 tccgtcccac gtatcgcagt ttcttgtcgc tcgttggttc acccaaaagt tctgtcaatt
     1381 ccaaggcaac ctggagcgag tacttgccaa ggtccgccac ggtggcaccg ttcctcacaa
     1441 ttccttgggc cttctccctc ggcaagtcgg acgaaagata ttccaagacg acctcatcga
     1501 gccattcagc gttaacaggc agtttttccg gctgttgaga accattcgta ctagactcgc
     1561 ccgatagcaa gaaagccact agagcgtcga tgacctcgga gctcgtcacc cggcgtggct
     1621 tggactcggc cggttcaaag acacgataaa tccaggcgtc gtcccgaaat tgtggttgcg
     1681 gcatccgggt agaaggggaa agtgtctgtg gcgaagtcaa ccaaagccgc agccactccc
     1741 gaccggtgtc agacgcaaga cgctgtccca ggtccgacaa gtcgggacca acgtccgaag
     1801 tgccttcggg aaaatcgcgg tggcgatgac aggccaggca accttgtgtg gcaaaaagga
     1861 ttcgccccgc gtctgccagg ctccgagaac ccaatttgtc ttgggagggt gcgcgtgtct
     1921 ccgcgaggtg agacggcagc ggacgacttt gccctaccaa gtaagccaca attccggcga
     1981 tctcaacctg ctcgaactgt ttacggtact gagctgaccc ctcacttaaa tggtctgtga
     2041 gaccaaagat ctgcggcatt c
//
LOCUS       DAOPTR010000770         2061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_42559_length_2061_cov_2.726820, whole genome shotgun sequence.
ACCESSION   DAOPTR010000770 DAOPTR010000000
VERSION     DAOPTR010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42559_length_2061_cov_2.726820"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2026)
                     /gene="cas9"
                     /locus_tag="PKI05_13025"
     CDS             complement(<1..2026)
                     /gene="cas9"
                     /locus_tag="PKI05_13025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01865.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HOA53169.1"
                     /translation="MQRINDLTLIDPKGDMRSLTQEERQTLYALAETHEKLTFDAIKK
                     VLGLTGEGWKFSMERGGEKELPGNKTAVRIRRIIGTKTWENLSESQRKELVDTIIAYL
                     SPEALAARLQKVWHFAPEVADKLSKVTLESGYHKYSRRAIRKLLPLLEKGDSLNTAIQ
                     KIYGGTQRKTDVHDLLPPVQEVVKDVRNPLVVRALTELRKVVNALIRKYGKPQLIRIE
                     LARDLKNPRKVREKRAEEMRQREKQRKAAAAKIQEVLGREAKGADIEKYLLAEECNWE
                     CPYTGRPITLEALLGDQPQFDVEHIWPFSRSLDNSFANKTLCYHQENRDVKKNRTPFE
                     AFGSNEQEWHEILARVERFKEPFKSEKLRRFKAESIPEDYPQRDLNDTRWISREAADY
                     LGHLYGGRIDPSGKQRIFAIKGQLTAIVRQAWDLNSILGTGPEKERNDHRHHAIDATT
                     GALADASLVQRISWAAARLPKTFSRITVQIDPPWEGFLNDVRQQIHNIVVSYRPNRRV
                     SGPLHDANFYTPPIGGQKPKHRKQLKDLSPKEVSKIVDDRIRTLVQETLERHGGKEPK
                     KVFNSPENLPVFNGRKVKSVRIWVSDHVMPVGQNHRIRYVIPSSKHHIEIYALLDDQG
                     NETAWQGKVVSLYEAFQRMKNGQPVVWRDHGPNTRFKFSLCKSDCIEWIGE"
BASE COUNT          403 a          537 c          562 g          559 t
ORIGIN      
        1 cctcgccgat ccattcgatg cagtcgcttt tgcacaatga gaacttaaat cgcgtgtttg
       61 gcccatggtc gcgccagacc acgggctggc catttttcat ccgttgaaag gcctcgtaga
      121 gactgacgac ttttccttgc cacgccgttt cgtttccctg gtcatcgagg agggcataga
      181 tttcgatatg atgcttacta ctgggaatga cgtagcggat gcggtgattc tggccgacgg
      241 gcatgacatg gtcagaaacc caaatccgga cggatttgac ttttctgcca ttaaacaccg
      301 gaagattttc tggggagtta aagacctttt tgggttcctt tcccccatga cgttcgaggg
      361 tctcttgcac aagcgttcta atgcggtcat cgacaatttt cgagacttct ttgggcgata
      421 aatctttcaa ttgtttgcga tgcttgggtt tttggccacc aataggtggg gtgtaaaagt
      481 ttgcgtcatg caaaggaccc gagacccgac ggttagggcg ataagagacg acgatattgt
      541 gaatctgttg ccggacgtcg ttcagaaagc cctcccacgg cggatcgatc tgcacggtga
      601 tgcgtgaaaa cgttttggga agtcgggcgg ctgcccaaga tatcctctgg accagcgagg
      661 cgtcggccag cgcgccggtg gtggcatcga tggcatggtg gcgatgatcg ttgcgttctt
      721 tctcgggccc cgtgccgaga atgctattga gatcccaggc ctgacgcaca atcgccgtga
      781 gttggccttt aatggcgaag attcgctgct ttcctgaagg gtcaatgcga cctccataaa
      841 gatggccgag ataatcggcg gcctctcggg aaatccagcg ggtatcgttg aggtcccgtt
      901 gcggatagtc ctctgggatg ctttcggcct taaagcgtct caatttttct gacttaaagg
      961 gctccttgaa cctttccacc cgggcaagga tttcatgcca ctcctgctcg ttgcttccaa
     1021 acgcctcaaa gggcgtgcgg ttctttttga cgtctctgtt ttcctgatgg tagcagagcg
     1081 tcttattggc aaaggaatta tcgagagagc ggctaaacgg ccagatatgc tccacatcga
     1141 attggggctg atcgccaagg agggcttcta gcgtgatcgg gcgtccggta taggggcact
     1201 cccaattgca ctcttcggcc aggagatatt tttcgatatc tgctcccttg gcctctcgac
     1261 ccaagacctc ctgaatcttg gcggcggcgg cttttctttg tttttcccgc tgacgcattt
     1321 cttcggcacg tttttcgcgg accttgcgcg gattcttgag atcgcgggcc aattcgatcc
     1381 ggatgagctg cggcttacca tatttgcgga tcagggcatt gacgaccttg cgcaactctg
     1441 tcagggctcg cacaaccaac gggtttcgga catccttcac gacctcttga acaggcggca
     1501 aaagatcatg gacgtccgtt ttccgctgcg tgcccccgta gatcttttga attgccgtat
     1561 tcagggagtc gcctttctcc aaaagtggca agagcttgcg gatagcgcgg cggctgtact
     1621 tgtgatagcc gctctccaaa gtgaccttgc tcagtttatc ggccacctct ggagcaaaat
     1681 gccaaacctt ttgaagacgc gctgcaagtg cctcgggact aagataggcg atgatggtat
     1741 cgaccagttc ttttcgttgt gactcgctga ggttttccca ggttttggtt cctatgattc
     1801 ttcgtatccg cacggcggtt ttattgccgg gcagttcctt ttcgccgccc cgctccatgg
     1861 aaaacttcca gccttcaccc gtcaacccaa gcaccttttt tatggcgtcg aaggtcagtt
     1921 tttcgtgtgt ctcggccagg gcgtacaagg tttgacgttc ttcctgggtc aggcttcgca
     1981 tgtcgccttt aggatcaatc aaggtcagat cgttgatcct ttgcatatag cgaatgcgtt
     2041 gcgcttcaag gcaagccatc g
//
LOCUS       DAOPTR010000771         2056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_42689_length_2056_cov_3.347826, whole genome shotgun sequence.
ACCESSION   DAOPTR010000771 DAOPTR010000000
VERSION     DAOPTR010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42689_length_2056_cov_3.347826"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="PKI05_13030"
     CDS             <1..1193
                     /locus_tag="PKI05_13030"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOA53170.1"
                     /translation="FPFPWLSGAGVAFRLAWAIARRASSGQRTDERLRDFLVQAVGLA
                     AIGTVADVVPLWDENRILVHYGCTHSLPKRPTLGLSYLQSLASQSSNGKTDSEWIAFQ
                     LAPRINAVGRLGQAALAVELLVTEKEDRAAELASYINSLNEQRKSLEQSIYLRALRQI
                     KEQCDPDTDPAFVLADTDWHQGVIGIVAGRLAEKFHRPVILISLSKTGMARAVGSARS
                     VPGLHLHEALTACAHHLERFGGHSQAAGLTIAEQSIPAFRHEFCQYVADHFDREQNLA
                     EIHIDCEFPLGVFTVEVVKQIERLAPFGMGNPRPIMCCENVTLVEPPRIVGQGQQHLS
                     LRVHHSGTTMRAIAFGAGDRSHELSCGEPFDLAFRPVITEFRGFERVELHVVDWRTPP
                     QRDG"
     gene            1272..>2056
                     /locus_tag="PKI05_13035"
     CDS             1272..>2056
                     /locus_tag="PKI05_13035"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HOA53171.1"
                     /translation="MASTRLSPKIYRWLKAELEDWKAEGLITAEQAEQIANRYAASAV
                     PGARANLITMVFSTMAGALVIIGIILFISFYWQQLPATVQMGLLVGGTIGFNALGLLV
                     RRFVPEGRILSDAIFLMAAIFFGASVFLVPQIAGRTMRVADGMLLWTTGTILLVLVLD
                     HPLFYAGLSFLVIGSLATAMVEDAYHLERTLYAILTGAVLCPVMIWAYGGRSVLSCLA
                     VGVAFCWWLVSFAFLQGFELPGILVGTYLALVGTAVWVLSEWS"
BASE COUNT          388 a          564 c          596 g          508 t
ORIGIN      
        1 cgtttccgtt tccctggttg agtggcgccg gggtggcctt tcgcctggct tgggcaattg
       61 cccggcgggc cagttccggc caacggacag atgaacgtct ccgtgacttt ctcgtgcagg
      121 ccgttggatt ggctgccatc gggacggtgg ccgatgtcgt accgctatgg gatgaaaacc
      181 gaatccttgt ccattatgga tgtacccata gcttgccgaa gcgtccgact ttgggattga
      241 gctacttgca aagtcttgcg tcacaatcgt ccaatggcaa gaccgacagt gagtggattg
      301 cctttcagtt ggcaccgcga atcaacgccg tcggccgatt gggacaagca gcgttagccg
      361 tcgagttgct ggtgacagaa aaggaagatc gggcggcgga gctggcaagt tacatcaaca
      421 gcctcaatga gcagcgaaag tctctcgagc aaagtatcta ccttcgtgcc ttacggcaga
      481 tcaaagaaca gtgcgatccc gataccgacc ctgcttttgt tcttgccgat accgattggc
      541 accaaggagt gatcggaatt gtggccggac gactggcaga aaagtttcac cgaccggtga
      601 ttctcatttc gctcagcaaa actggcatgg cgcgggccgt gggttcagcc cggagtgttc
      661 cgggattgca tctccatgag gccctcaccg catgtgccca ccacctcgag cgttttgggg
      721 ggcattctca ggctgccggg ctgactattg cagagcaaag catccccgct tttcggcatg
      781 agttctgcca gtatgtcgcc gatcattttg accgagaaca aaacctcgcc gagatccata
      841 tcgactgcga atttccgttg ggcgtgttca ctgttgaggt ggtgaaacaa atcgaacgcc
      901 tggccccgtt tggcatgggc aatccgcgac ctatcatgtg ctgtgagaac gtgacacttg
      961 tcgaaccacc ccgcattgtg ggacaaggtc aacagcatct ttcgcttcgg gtccaccact
     1021 ccgggacaac catgagggcc atcgcgttcg gggccggtga tcgaagccac gagctctcgt
     1081 gcggagaacc gtttgatctt gcctttcgcc ctgtgatcac cgaatttcgg ggttttgagc
     1141 gcgtcgaatt gcacgtcgtt gattggcgaa caccgccgca gcgcgacggt tgatcgccaa
     1201 tcctcgaagg tgtatgctct cgtaaagctc cgcgttactc gtcaacgaaa agaagggatg
     1261 gaacgtactc cgtggcaagc accagattgt cacccaagat ataccgctgg ctgaaggcag
     1321 agcttgaaga ctggaaagcc gagggtctta tcacagcgga acaggctgag caaatcgcca
     1381 atcgctatgc ggcttccgcg gtccccggtg cccgggcaaa cctcattacg atggtcttct
     1441 cgaccatggc cggggcattg gtcattattg gcatcatcct cttcatttcg ttctattggc
     1501 agcaattgcc agccaccgtg cagatgggac tgcttgttgg cggcacgatc ggtttcaatg
     1561 ccctgggtct ccttgtccgg cggtttgtcc ctgagggccg catccttagt gatgcaatct
     1621 tcctgatggc tgccatcttt ttcggggcat ctgtctttct tgttccccaa atcgccggga
     1681 ggacgatgcg tgtcgccgac gggatgttgc tctggaccac cgggacaatc cttttggtgc
     1741 tagtgcttga ccatcccctt ttttacgccg gcctgagttt tcttgtgatc ggttccctgg
     1801 ccacggctat ggtcgaagat gcctaccatt tggagcgcac gctgtacgca atcttaaccg
     1861 gagccgtctt atgccccgtc atgatctggg cctatggggg acggtcggtc ctgtcatgtc
     1921 tggctgtagg cgtggcgttt tgctggtggc ttgtcagttt cgctttcctt cagggatttg
     1981 agcttccggg tattttggta ggcacttact tggcgcttgt cggcacagcg gtctgggtgc
     2041 tctcggaatg gtccca
//
LOCUS       DAOPTR010000772         2055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_42726_length_2055_cov_2.800500, whole genome shotgun sequence.
ACCESSION   DAOPTR010000772 DAOPTR010000000
VERSION     DAOPTR010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42726_length_2055_cov_2.800500"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="PKI05_13040"
     CDS             complement(<1..624)
                     /locus_tag="PKI05_13040"
                     /inference="COORDINATES: protein motif:HMM:NF014518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
                     /protein_id="HOA53172.1"
                     /translation="MGRIQTNVGLITGVPIGETVNQLMLIESRPKENLESANKKIDAE
                     RTAITELSVLFLAAQYPIKNLLKDAVYTKRSATSSNPSALNVRVTGTPSVGTYVFTPI
                     RTAQSEQWLSSGVRESTTPLGGGVLSFRYGPSIDRPLALDAIRGGQGFERGVIRITDR
                     SGASAEIDLTTVQTLDDVIEAINRNMRINVLAEVVGDHIRLTDRTGMT"
     gene            966..1991
                     /locus_tag="PKI05_13045"
     CDS             966..1991
                     /locus_tag="PKI05_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53173.1"
                     /translation="MANVLYQQQRTADIPRGFTRLGRSHSAPWMLDRRLSVALILTVL
                     VFPAGCSLFQKPEWRPLVHNPFPQIHKIAVAPFFNLSTDPTLDGRKVAIAYYNELQEV
                     PGFEVIPVGVVERAMFQHRISLNNPQDVRHLAQLLGADAVVIGAVTEYSPYYPPRCGL
                     QVEWYAANPGFCPVPPGYGLPWGTKEEKDIPSRLVLESQLAYAREALRNQSPQAADAS
                     ATDAAAQPGFEIPQTGEGKSQASLSPEGLIPKLAQAGDSACGAGEEPFMKHTRVYNGG
                     DSQLVKLLQDYAKFRDDARLGGWQSYLTKSEEFLRFCCRVHIWEMLTARGGADESELV
                     WSTTSGR"
BASE COUNT          449 a          565 c          589 g          452 t
ORIGIN      
        1 cgtcatcccc gtgcggtcgg ttaggcgaat gtggtcgccc accacctcgg ccaagacgtt
       61 gatccgcatg ttcctattga tggcctcaat cacatcgtca agcgtttgca cggtggtcag
      121 gtcgatttcc gcggaggcgc cacttcggtc ggtgatgcgg atgacgcctc gctcaaagcc
      181 ttggccgcca cgaattgcat ccaaagccaa tggtcgatcg atacttgggc cataacgaaa
      241 agataacacg cccccgccca ggggcgttgt gctctcccgc acgccgcttg ataaccactg
      301 ctcggattgc gccgtacgga tcggtgtgaa cacataggta ccgacgcttg gggtgccggt
      361 gacacggaca ttcaacgccg agggattgct gctcgtggcg gaccgcttgg tgtaaacggc
      421 gtctttcaaa agatttttga tggggtactg agctgccaaa aagagcacgg aaagttccgt
      481 gatggccgtc cgctcggcgt cgattttctt gttggccgac tcgaggttct cctttgggcg
      541 ggactcaatc aacataagct gattgacggt ctccccaatg gggacgcccg ttatcaagcc
      601 gacattggtc tgaatccggc ccatgcgaag tcctcgcgca caatttcacc cgttggcaaa
      661 gtccgccttt tttgacatcg accatcacgg cggcctgcat tgaggaagac ccttcccata
      721 agaaaaccta gttcccacgc tgttcgttaa aatgcaaaag atgggaaatt caaaacagac
      781 gactacgtcc agccaccgtt tttggttgag tttgccaata aaaatgtttg gagaatctgc
      841 gggaacattg ccgataagtc aatcgcgttg ggcagaacgg cttgcgccgg cagggtcgat
      901 tgtgccggag gcagggccct atcagatttg cgggaacaat cgagattgga ccagttcggc
      961 tcacaatggc aaacgtgctt taccaacaac aaaggacggc cgacataccc cggggattca
     1021 cccggttggg acggtcccat tcggcccctt ggatgttgga tcgccggtta tcggtggcat
     1081 tgattttgac agtcctcgtg tttcccgcag gttgctcgct attccagaag ccggaatggc
     1141 ggccccttgt tcacaatcct tttcctcaaa tccacaagat tgctgtagcc ccgtttttca
     1201 atctttcgac agatccgact ttggacgggc ggaaggtggc cattgcttac tacaacgagc
     1261 ttcaagaggt tcccggcttt gaggtcatcc ctgtgggggt ggtcgaacgg gcaatgttcc
     1321 agcaccgcat cagtctcaat aatccccaag atgtccgcca tcttgcccag ttgctggggg
     1381 ccgatgccgt cgtgattgga gccgtgacgg agtattcccc ctactatccc ccgcgatgtg
     1441 ggcttcaggt ggaatggtac gcggcaaacc ccggcttttg ccccgttcca ccaggctacg
     1501 gtttgccatg gggaacgaag gaggaaaaag atatcccctc ccggctggtc ctggagtcac
     1561 agctagccta tgcccgcgag gccctgcgga accagtcgcc ccaagctgcg gatgcttccg
     1621 cgacagatgc cgctgcgcag ccgggcttcg aaattccaca gacaggggag ggaaaaagcc
     1681 aggcttcgct ctcgcccgag gggctcatcc ccaaacttgc ccaggcagga gattctgctt
     1741 gcggagcggg cgaagagccc ttcatgaagc acacacgggt ttacaacggg ggcgatagcc
     1801 aactggtcaa attgcttcag gactatgcga agtttcggga cgatgcccga ttggggggat
     1861 ggcagtcata tctcacaaaa agcgaagagt ttctccggtt ttgctgccgg gttcacattt
     1921 gggaaatgct aacggccagg ggtggggccg acgaaagtga actagtttgg agcacgacat
     1981 ccggccgata aaaggaataa ggcattcaat ttgggtagaa ttggtgcagg caggaactca
     2041 agctcgcagc tattg
//
LOCUS       DAOPTR010000773         2053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_42775_length_2053_cov_2.777778, whole genome shotgun sequence.
ACCESSION   DAOPTR010000773 DAOPTR010000000
VERSION     DAOPTR010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42775_length_2053_cov_2.777778"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..163
                     /locus_tag="PKI05_13050"
     CDS             14..163
                     /locus_tag="PKI05_13050"
                     /inference="COORDINATES: protein motif:HMM:NF016223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOA53174.1"
                     /translation="MGQLLDKVHDLPDIRADLVARIRAEIAAGTYETEEKLQVALERL
                     LDEIG"
     gene            166..669
                     /locus_tag="PKI05_13055"
     CDS             166..669
                     /locus_tag="PKI05_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOA53175.1"
                     /translation="MSKQYQLGPIEIPEPPLLRFAEYLRAQVKRMTSQRQIIVNEVFS
                     HHDHFDAEDLINHLRDRLAAREVSRPTIYRTLGELVEAGLLRRMILRERYVYEHQYGY
                     PEHDHLYCQSCHQLFEFHTDALIKIREAVAREYHFTPTSHRMIIMGICADCQRKAEQS
                     PGVSKRA"
     gene            complement(808..>2053)
                     /locus_tag="PKI05_13060"
     CDS             complement(808..>2053)
                     /locus_tag="PKI05_13060"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOA53176.1"
                     /translation="FGFASREWLKGFLDPKQIVTEKYYGNTRFAAGAMVRYVEERFAK
                     LPEEEQAAIIAALSAEARLPEQRKIDNEQSAVIARGRELIVRECTRCHEFHGSGPVGQ
                     APVLTGYGSREWLLGLVASPEHVAFYGLRNDRMPVYVEDSLRPEKNRITDVQLSVIVD
                     FIRGDWPRQSEGSQDQDADDTPPVTVAYALGRWDASKKPLPSRPTQDARAEARWLWNK
                     ELCGLCHAHTGEGPDNMPAVSSSAPDLGGFASRDWIAGLLDPKQVDSPKYFGNSGFAK
                     GSMVKFVKGNLRELIDEIGKDEFQKLIDALAAEAKKDWKDGEDPPEPDEDTVLLFEDF
                     TCADCHRFYSTGSLGQAPDLTGYGSKRWLAEFIADPKARKFYRQTNDGMPSYQAFPDH
                     PEKNLLTPDEIKILAEYLAPVE"
BASE COUNT          464 a          620 c          499 g          470 t
ORIGIN      
        1 gcatttatcc ccggtggggc agcttctgga caaggtgcac gacctccctg atattcgcgc
       61 cgacctggtc gcgcggattc gcgccgaaat cgctgccggc acgtatgaaa cagaagaaaa
      121 actgcaagtg gctcttgaac gtcttctcga tgagatcggt taggcgtgtc gaagcagtat
      181 caacttggcc cgatcgaaat tcctgaaccg cctctcctcc gttttgcgga atatttacgc
      241 gcgcaggtaa aacggatgac gtctcagcgt caaatcatcg tcaatgaagt gttcagccac
      301 cacgatcact tcgacgctga ggacctgatc aaccatcttc gtgaccggct cgctgctcgc
      361 gaagtcagcc gtcccaccat ttaccggaca ctgggcgaac tggttgaggc tggacttttg
      421 cgtcgcatga tcttgcgtga acggtacgtt tacgagcatc aatacggcta ccccgaacac
      481 gaccatctct attgccaatc gtgtcatcaa ctgtttgagt tccacaccga cgctcttatc
      541 aagatccggg aagccgtcgc tcgggagtac cactttaccc ccacgtcgca ccgcatgatc
      601 atcatgggta tctgcgccga ctgccaacgt aaagcagagc aatcgcccgg tgtcagcaaa
      661 agagcgtagc tacccggatc gttcagccgg gtacgctgca tccgcccgtt gtacctcgcc
      721 cccctgagat aaatccgtcg gcaaaagaga attcaccgaa cgtagatttc tttcctacgg
      781 aacccccaca caatttgggg ggcattttca ctcgacgggt gccaaatact ccgcaagaat
      841 cttgatctcg tctggtgtca aaagattctt ttcgggatga tccgggaagg cttggtagga
      901 gggcatcccg tcgttggtct ggcgataaaa tttcctagcc ttgggatcgg caataaactc
      961 cgccaaccac ctcttggagc cataaccggt caagtccggg gcttgaccta aacttcccgt
     1021 ggaataaaac cgatggcaat ccgcgcacgt aaaatcttcg aagaggagaa ccgtatcttc
     1081 atcaggttct ggtgggtcct caccatcctt ccagtctttt ttggcttccg ccgccaaagc
     1141 gtcaatcaac ttttgaaatt cgtcttttcc aatttcatca atcaactctc tcaggttgcc
     1201 tttgacaaac ttcaccatac tccctttggc aaatccactg ttgccaaagt acttgggcga
     1261 atcgacctgt ttgggatcga gcaagccggc aatccaatca cgggatgcaa atccaccaag
     1321 atcaggtgcc gaggaactaa cggcgggcat gttatctggg ccttcgcctg tatgggcatg
     1381 acacaacccg cacaactcct tattccaaag ccatcttgcc tcggcccgcg cgtcctgtgt
     1441 tggccttgat ggcagcggct ttttcgaggc atcccatctc cccaaggcgt aagccaccgt
     1501 caccggcggg gtatcgtcag catcttgatc ctgcgaccct tccgattgcc taggccaatc
     1561 gccacggatg aagtcaacga tcacgctcaa ctggacatcc gtgatgcgat tcttctccgg
     1621 gcgtaagctg tcctcgacgt agacaggcat ccgatcgttc cttaggccat agaacgcaac
     1681 atgttccggt gaggcaacaa ggccgagaag ccattcccga gaaccgtaac ctgttaaaac
     1741 gggggcttgt cccacgggcc ccgatccatg gaattcgtga catcgcgtac actcgcggac
     1801 aatcaactcc cttccccggg cgattactgc agactgctca ttatcgatct tccgctgctc
     1861 gggcagtcgt gcctccgccg aaagggccgc aatgatggcc gcttgctcct cctcagggag
     1921 ctttgcgaat cgttcctcga catagcggac catcgcgccc gccgcgaaac gcgtattccc
     1981 gtagtatttc tccgtcacaa tctgcttggg atcaaggaac cccttaagcc attctcggga
     2041 ggcaaaacca aat
//
LOCUS       DAOPTR010000774         2050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_42846_length_2050_cov_4.378446, whole genome shotgun sequence.
ACCESSION   DAOPTR010000774 DAOPTR010000000
VERSION     DAOPTR010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42846_length_2050_cov_4.378446"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PKI05_13065"
     CDS             complement(<1..676)
                     /locus_tag="PKI05_13065"
                     /inference="COORDINATES: protein motif:HMM:NF037334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53177.1"
                     /translation="MLRSLAALLVFLGVTRQVFAQRQMEDLDRGLVAIDRGDGTVMVS
                     WRLLADDPDDLAFHVYRRQEDGTWIRLTDSPLLDRTNFVDEDRDSPWPRFYQVRNVIS
                     GKETGETATVEVAKGQTHWVIPLQTLPGYTPNDASVGDLNGDGRYELVLKQEKRPRDN
                     SQRGPTGQTLLEAYTFDGKFLWRINLGPNIREGAHYTPFLVYDFDGDGRAEVVCRTSD
                     GTVDGTG"
     gene            1072..1824
                     /locus_tag="PKI05_13070"
     CDS             1072..1824
                     /locus_tag="PKI05_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53178.1"
                     /translation="MIEFLCPNGHRIRCSEDRAGKPAKCPKCSVKFLIPTLEEIRAAQ
                     GEDAGSNATFSRDRTRIQFLCPNGHRLFGSADLQGRPGQCPECGVRFRIPVIEELTDD
                     DADEAVPLSVAPEPSAGPQLATGTGLGDSAIVPESGIHLELFSPRSAGDSRTPESSIS
                     SPTSIKGSHLARIFERLWNGRAAGSVVKVQLVDGQAFTVTKYSASSNDHEYAAFVVEG
                     SAFGGGLVVVPWQSVRMIYVSGVKDIPSQLLD"
BASE COUNT          457 a          512 c          599 g          482 t
ORIGIN      
        1 ggcccgtacc atcgaccgtg ccatcggagg tgcggcaaac gacctcggcg cgcccatcgc
       61 cgtcgaagtc gtacacaagg aacggcgtat aatgggcccc ttcccggatg ttgggaccaa
      121 gattgatccg ccaaagaaac ttaccgtcaa aggtgtaagc ttccaggagc gtctggcctg
      181 tgggcccgcg ttgcgagtta tcgcgaggcc gcttctcctg tttgaggaca agctcatagc
      241 ggccatcgcc gttcaaatct cccacactgg catcgttggg tgtgtagcct ggtaaggtct
      301 gcaaaggaat gacccaatgg gtttggcctt tcgcgacctc gacggttgcc gtctcgcccg
      361 tctctttgcc tgagataaca tttcgcactt ggtagaatcg aggccagggg ctatcacgat
      421 cttcatcgac gaaattggtt cgatccaaaa gcggagaatc ggtcaggcga atccatgtgc
      481 catcttcctg gcggcgatac acgtggaagg ccaaatcatc ggggtcgtcg gcaagcaacc
      541 gccagcttac cattaccgtc ccatcaccgc ggtcgatggc gaccagaccc cgatcaaggt
      601 cttccatctg acgttgggca aaaacctgcc gggtgactcc gaggaaaaca agaagggcgg
      661 caagagatcg taacataaaa tggatcgctg ctggcaagac tgagcggtgc ttatggttca
      721 gattgacaat catcgttagc aaaaacgtgg accggtgcga aaggtttctt caaaaccagc
      781 gagatatcgc cttttgttat ccctctattg ggtggttaat ttgtgctatt tgctcaaacg
      841 aattggcaag aatcgaaagt tcgacaattt ccgtttcaag gagcaagcgg taaactaaat
      901 acgagaggat tttgcttgca aaggtcatgg tatttcctcc ccgggagaat gggaagagac
      961 ccgttcgacg tgaaaggccg tggcctttta ttgtgatggc aacaatttgg caaggttatg
     1021 ttttagggct tgaccgggag actttttgaa caaggggtgg agatgccggc catgatcgaa
     1081 tttctttgtc cgaacggcca tcgtattcgt tgctcggaag atcgggcagg gaaaccggcc
     1141 aagtgtccca agtgcagtgt gaagtttctg attcccacgc tggaggagat tcgggcagct
     1201 caaggcgaag acgccggttc gaacgccacg ttttcacgag atcgtacccg cattcagttc
     1261 ttatgtccca acgggcatcg gctctttgga tcagcagatc ttcaggggcg tcccggccag
     1321 tgtcccgagt gtggtgtccg gttccgcatt cctgtgatcg aggagctgac cgacgacgat
     1381 gcagacgagg cggtgccgct cagtgtggct ccggagcctt cggcaggtcc ccaactggcg
     1441 acagggactg ggttagggga ctcggccatc gttccggaga gtggcatcca cttggagttg
     1501 ttttcgccac gatcggccgg cgatagtcga acgcccgaga gttctatctc atcgccgaca
     1561 tcgattaagg gcagccattt agcacggata tttgagcggc tttggaacgg tcgcgcagcg
     1621 ggtagcgtgg tcaaggtcca attagtagat ggccaggcct ttacggtaac gaagtacagc
     1681 gcttcttcga acgatcacga atatgcggct ttcgttgtgg agggttcagc ttttgggggt
     1741 ggcttggtcg ttgttccttg gcaaagcgtg cgcatgattt acgtcagcgg cgtcaaagat
     1801 attccatccc aattgcttga ctgatttggc atgtgggcct tcgagagtag gacccattcg
     1861 ggttgatgag ggcgaaaaat cgcttgtcca tgcaactgcc aactttgtga tgaaaacttt
     1921 ctggcatatt gagctgattt cgtcgcctgt gcccgagatc catttagaac ttatcccaaa
     1981 gagtccccct ctccctccgg aagagggttg gggtgagggc aagcgaatgt cggaatcgat
     2041 tgagaaaacc
//
LOCUS       DAOPTR010000775         2043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_43051_length_2043_cov_2.824950, whole genome shotgun sequence.
ACCESSION   DAOPTR010000775 DAOPTR010000000
VERSION     DAOPTR010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43051_length_2043_cov_2.824950"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PKI05_13075"
     CDS             complement(<1..489)
                     /locus_tag="PKI05_13075"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOA53179.1"
                     /translation="MEREFTPGALRALAAAATWQLGETFDGLRPVTILMGLLAEPECR
                     AAALLRAIGIEETAILNRWPELRRSDRPPVITPESPPAFHAEAESILEAVDTYMDWGD
                     WPPIISTEHLLLGLVAGDEPVACWLREMGLEFHDLLRQILERYGTATKWRSPTVPLEL
                     SSS"
     gene            complement(617..>2043)
                     /locus_tag="PKI05_13080"
     CDS             complement(617..>2043)
                     /locus_tag="PKI05_13080"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOA53180.1"
                     /translation="QHRLLQWMRDGVIFVDTHKVILQWNYGAERLSGIMADAVRLRQW
                     SPTILGFRNERGQPIDESDCPILTAINCGTQVLRRLTIKGRAGKPVSVDVHVVPVILD
                     DGVILGAVAILHDASSEISLEQRLEYFRDRVSKDPLTQLANRAEFDRMLPLFVEQYQR
                     RGLTFSLIICDLDRFKQVNDTYGHQAGDDAIRSLAAVFRSFYRNGDLIARYGGEEFVM
                     LCAGCDIATATRRAEEMRAALERTPQPRLNNRVVTASFGVTEVQPGDTPETILRRADR
                     ALLMAKSRGRNMVVQLGAGNNGESIQKPVSRWWSWPHNSQELIWREDLISPIPLNLAM
                     EKLHGFIADHLGRVVHLDRHHLEIEIEDRNPLRTRRSADRPICFGLRVDLSEVSTEVT
                     QPSRTRLHLEVWPKSGRERRRREIVLRAHDLITSFHSYLMVSEITTEASATLWGRFKR
                     FVARCLGTQPERPKTDVQQPGHSL"
BASE COUNT          458 a          580 c          599 g          406 t
ORIGIN      
        1 agaggaagat aactccaggg ggacagtcgg actacgccat ttcgtggccg tcccatatcg
       61 ttccaagatt tgacggagta gatcgtgaaa ctccaggccc atttcgcgca accaacaagc
      121 cacgggttca tctccggcca cgagcccaag gaggaggtgc tcggttgaga tgatcggcgg
      181 ccaatctccc caatccatgt atgtgtcaac cgcctcgaga atggactccg cctcggcgtg
      241 gaaagcgggc ggtgattctg gcgttatcac gggaggacga tctgagcgcc tcagttcggg
      301 ccagcggttg aggatggcag tttcctcgat accaatcgcc cgaaggagtg cagcagcccg
      361 gcattccggc tctgccaaaa gccccatgag aatggtgacc ggtcgtaagc catcaaacgt
      421 ctcaccgagt tgccacgttg ccgcagcggc caaagcccgc aacgcaccgg gagtgaattc
      481 acgttccatg gtggggttca aattctgtga gataggaaga atccaactct tctgattagc
      541 ttacaaaggc ctttcgtgga gagaaagcga tcctacgacg aactccagga ggacccgcgg
      601 ggctggctca gaccgttcac agcgaatggc ccggctgttg cacgtcggtt ttcggccttt
      661 ccggctgggt accgaggcag cgagcgacaa acctcttgaa ccgaccccaa agggtggcac
      721 tcgcttccgt tgtaatctcg ctcaccatca agtaggagtg aaaacttgtg atcaagtcgt
      781 gcgcacgaag gacaatctcg cgtcggcggc gttcgcgtcc actcttcggc caaacctcca
      841 gatgaaggcg tgtccgggag ggctgtgtga cttcggttga cacctcgctc aggtcaacgc
      901 gaagtccaaa gcaaatgggg cggtcggccg aacgtcgcgt ccgcaggggg tttcgatcct
      961 cgatctcgat ctccagatgg tggcgatcca agtggacaac acggcccaaa tggtcggcaa
     1021 tgaacccatg gagtttctcc atcgccagat tgagtgggat aggactgata agatcctcac
     1081 gccagattaa ctcctgggag ttgtggggcc aagaccacca acgggaaacc ggtttttgga
     1141 tcgattcccc attgttcccg gctcccaact gcacgaccat gtttctgccg cgcgacttgg
     1201 ccatcagcaa ggctcggtcg gcacgacgga ggatggtttc aggggtgtcg cccggttgga
     1261 cttccgtaac gccaaaactg gcggtgacga cgcggttgtt gagtcggggc tggggagttc
     1321 gctccaacgc agcacgcatt tcttcggctc gccgtgtcgc cgtggcaatg tcgcatccgg
     1381 cgcacagcat gacgaactcc tcgccgccat atcgggcaat gaggtcaccg ttccgataaa
     1441 agctgcgaaa gacggcggca aggctacgga tggcatcatc accggcctgg tggccgtaag
     1501 tgtcatttac ctgtttgaaa cggtccaaat cacaaatgat caagctaaat gttagcccac
     1561 gtcgctgata ctgttccacg aacaggggca acattcggtc gaattctgcg cgattggcca
     1621 attgggtcag gggatcctta gaaacccgat cgcggaagta ctcgagccgc tgttcgagag
     1681 aaatctctga cgaggcatcg tgcaggatcg ccacggcccc caagatgaca ccatcgtcga
     1741 gaatcaccgg gacgacatgc acatcgaccg atacaggttt gcccgcacgt cccttgatcg
     1801 tcaaacgccg caggacttgg gtcccgcagt taatggctgt gagaattggg caatccgatt
     1861 cgtcgatcgg ctgcccgcgc tcgttacgaa aaccgaggat ggtcggtgac cactgtcgca
     1921 aacggacggc atcggccatg ataccactga ggcgttcggc gccatagttc cattggagaa
     1981 tgaccttatg ggtatccaca aagatgacgc cgtcgcgcat ccactggagc aggcgatgct
     2041 gaa
//
LOCUS       DAOPTR010000776         2039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_43160_length_2039_cov_3.899698, whole genome shotgun sequence.
ACCESSION   DAOPTR010000776 DAOPTR010000000
VERSION     DAOPTR010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43160_length_2039_cov_3.899698"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1853)
                     /locus_tag="PKI05_13085"
     CDS             complement(<1..1853)
                     /locus_tag="PKI05_13085"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA53181.1"
                     /translation="MACKVRSLLLVTILGIVPFVCLCPTLRAWANEGKPYLRPNIVYI
                     VSDELGYYELSCMGHPHFQTPNIDRLAAEGIRFTQALAGSSLCAPTRCCLMTGKHSGH
                     TSIRTNGGGTPLRPEETTIASVLKQVGYATGGFGKWGCGGRGSTGVPELHGFDIFFGY
                     YDQVHAHSYYPPYLIRNSEEVPLPGNRGLSEGTIYSHYLIVEEAKKFIRENKDRPFFC
                     YLPVTPPHGIHDIPETDPAWAIYRDKPWPLDARKYAAMVNMVDRHVGEIVDLLKELGL
                     REKTIIFVSGDNGGAEYFRDADHPRGFHAPNVNPVTGVQFRGGKGNLYEGGLRVPMIV
                     CWPGRIKPGRVSDLLWYFPDVLPTLAEIAGATPPPDIDGISIVPELLGEEVVGRKQSQ
                     HDYLYWELSGQIAVRRGTWKAIRPGAGKPWELYDLSKDISETMDLSAEHPKILKELIA
                     LAEKAHQPAVEGDFLQPELNEKDRRAKFGDAVPPEKINVALVQNMPQAGLIPRKNYRI
                     VSCSSESISNGRLAQHAIDGNPRTWWHTQFSPEVKRPPHELVIDLGSEHIIRGVRYLA
                     RQDATWNGTIKDCDLTVSATPEFPDEPTVKLQFSKTRECQERLFPEVRGRYV"
BASE COUNT          447 a          561 c          558 g          473 t
ORIGIN      
        1 acgtaacggc ctcggacctc tgggaataac cgctcctggc attccctcgt cttgctgaat
       61 tgtaatttca cggttggttc gtctgggaac tccggtgttg cactgaccgt caggtcacag
      121 tccttgatcg tcccattcca tgtggcatcc tgtcgggcga gatagcgaac accacggatg
      181 atgtgctcac tccccaggtc gatgaccaac tcgtgaggtg ggcgtttgac ctcaggcgaa
      241 aactgggtgt gccaccaagt ccggggattg ccgtcaatag cgtgctgggc caaacgaccg
      301 ttggatatcg attcgctgct gcacgatacg atccgataat tcttgcgagg aatcagccca
      361 gcctgcggca tattttggac aagcgcgacg ttgatttttt cgggcggaac agcgtcgccg
      421 aacttcgcgc ggcggtcttt ttcgttcaac tccggttgca ggaaatcacc ttccaccgcc
      481 ggctgatggg ccttttccgc cagggctatc aattccttga gtattttcgg atgttcagcc
      541 gacaggtcca tcgtttcgct gatgtccttc gacagatcat aaagctccca cggttttccc
      601 gcccctggcc gaatcgcctt ccaggttcct cgccgtacgg ctatttgtcc actcaattcc
      661 cagtacagat aatcgtgctg gctttgtttg cgaccgacta cctcttctcc aaggagctcg
      721 ggaacgatag atattccgtc aatgtcgggc ggaggcgtcg ccccggcaat ctcggcaagc
      781 gtgggcagga catcggggaa gtaccaaaga aggtcgctaa cccgaccggg tttgatccgg
      841 cctggccagc agacaatcat ggggacgcgg agtccgccct catacagatt gccttttcct
      901 ccgcggaact ggacgccggt aaccgggttc acattgggag cgtggaaccc tctgggatga
      961 tcagcatctc ggaaatactc ggcgccaccg ttgtcaccgg atacgaaaat gatggttttc
     1021 tcacggagtc ccagctcctt gagaagatcc actatttccc ccacatggcg gtccaccata
     1081 ttgaccatgg cggcgtattt tctggcatcc agcggccaag gtttgtcgcg gtagatcgcc
     1141 caagcggggt cggtttcagg aatatcgtga ataccatgcg gtggtgtcac gggcaaatag
     1201 cagaaaaagg gacgatcctt gttctcacgg ataaatttct tggcctcttc cacaatgaga
     1261 taatgcgagt atatggtgcc ctcgctcaag ccgcggttgc ctggcagggg gacctcctca
     1321 ctgttgcgaa tcaggtaagg cggatagtag gagtgggcgt gaacttgatc ataataaccg
     1381 aaaaagatgt cgaacccgtg aagttctggc acccctgtgg aaccacgtcc gccacacccc
     1441 catttgccaa atccaccagt ggcataacca acctgtttga gcaccgaggc gattgtggtt
     1501 tcctccggtc ggagtggtgt tccaccaccg ttggtgcgga tcgaagtatg tcctgagtgt
     1561 ttacccgtca tcaaacagca tcgtgttggc gcgcagagtg agcttccggc aagtgcttgt
     1621 gtaaaccgaa tcccctcagc ggccagacga tcgatattgg gcgtttgaaa gtggggatgt
     1681 cccatgcacg agagctcgta ataacccaac tcatcggaaa cgatatacac aatgttgggc
     1741 cgcaaatagg gctttccctc gttggcccaa gccctcaggg tcgggcacaa gcagacaaat
     1801 ggaacaattc ccaagatggt aacaagcagc aatgaccgaa ctttgcacgc catcgagtaa
     1861 tcctccccaa aaaagcgatc aaaactgtcc acaaagtatt gtcgattata ggtgtaggcc
     1921 tcatggtttc accacaggat tgccttggcg tcgctgggtg acagggatgg ccgcttcggg
     1981 gtactgggca agaatcttct tcagttgggc cgcgacatcg ggcagcttgt caatgaggt
//
LOCUS       DAOPTR010000777         2038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_43194_length_2038_cov_2.891578, whole genome shotgun sequence.
ACCESSION   DAOPTR010000777 DAOPTR010000000
VERSION     DAOPTR010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43194_length_2038_cov_2.891578"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(465..1931)
                     /locus_tag="PKI05_13090"
     CDS             complement(465..1931)
                     /locus_tag="PKI05_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOA53182.1"
                     /translation="MADISAEVLAERAHDVGLLTDWEIRQIWSELGRKSVSLDEFIQF
                     LVRREYLTNYQAEKLLNGDRDGFFYRDYKVLYLVGAGTFARVFRAVHKDTKEVVALKV
                     LRRRYSDNPNCYQQFVREGRLGMALRHPNIVPVFDVCSYNGTHFLVMEFVEGQNLREF
                     VKVRKKLDPIVATRIMVDITSGLDYAFRQGLTHRDLKLSNVLISSNGTAKLVDFGLAA
                     VDEFLEAALPEIPANARAIDYAALEKATGVPRNDPRSDIYFLGCMYYHMLTGDPPLSE
                     TKDRMKRLSRNRFYEIIPIHQREPSTPKVVAGVVNKAMSLDPETRYQTPGEMLIDLRI
                     AAKRLAQGEADVDLTAAKGGNADSNQPTVLVIEPNVEMQNVLREGLKKLGYRVLIISS
                     PQRALDRIRDETEGIDCIVIDAGELGESAVTAFNQIGDDPRTHMIPAVLMLEQHQRDL
                     AGMANRSDHRQVVTLPLTMKQLRGVVAGLVSKQRDFAK"
BASE COUNT          456 a          560 c          536 g          486 t
ORIGIN      
        1 tttgtccaat acattgcgtt ttacctcgat cgttccgtgc catgtcgtca caacgtccgg
       61 ctccgcgtcg aaattgctga tcttcgacac gttgccgggc tcgtatttgg gcttgttctc
      121 gccataagcc ccgaaaacga cgattctaga ccgcagagcg taaattggac aagatcggcc
      181 aaatggaaga aacctctcga tggtgcgaag agcagtactt ctcggaaatc gctcgcggag
      241 gggatccgac catcgtcggg acggcttttt atgcaagacc atctagatgg aaaagcgact
      301 caacggggct gccttacgcc ttctcttgtc cttccggcgc agacccgttg tcttttgatc
      361 ccaaagcaaa cgacgccggc ttttccctct ggtacggccc ccctcatgac gaaagtgcac
      421 tttcttgggg acaaaatgct tgttccgtga gacaaagaca gatattactt ggcgaaatcg
      481 cgctgttttg ataccaaccc agcaacgaca ccgcggagtt gtttcatcgt cagcggcaac
      541 gttacgactt ggcggtgatc agaccggttg gccattcccg ccaagtcacg ctggtgctgc
      601 tcaagcatca ggactgccgg aatcatgtgc gtgcggggat catcgccaat ttggttgaaa
      661 gcggttactg ctgattcacc caattcaccg gcatcaatga cgatgcagtc aatcccctcg
      721 gtctcatccc tgatccggtc gagtgctcgc tggggtgacg aaatgatcag gactcggtag
      781 ccaagtttct taagcccttc gcgcaagaca ttttgcatct cgacattagg ttcaatgacc
      841 aaaactgtgg gttggttcga atcggcgttg ccccccttgg ctgcggtcag gtcaacatcc
      901 gcctcacctt gggcgagccg tttggcggcg atgcggaggt cgatgagcat ttccccgggg
      961 gtttgatatc gggtctcggg atcaagagac atcgccttgt tgacgacacc cgccacgact
     1021 ttcggtgtcg agggctctct ttgatggatc ggaattatct catagaaacg gttccggctt
     1081 aaccgtttca tgcggtcctt tgtttcggaa agcggtgggt cgcccgtaag catgtgatag
     1141 tacatacagc ctaagaaata gatatcgctc cgcggatcgt tgcgggggac acctgtcgcc
     1201 ttctcaagtg ccgcatagtc aatggcccgc gcgttggcgg ggatttcggg caaagcagct
     1261 tctaggaatt catcgaccgc agccagtccg aaatcgacca gcttggccgt tccattgctg
     1321 ctgataagga cgttgctgag tttcaagtca cggtgggtca acccttgacg aaaggcgtag
     1381 tccagcccgg atgtgatatc taccatgatc cgggtggcga cgatggggtc caatttcttg
     1441 cggactttaa cgaactcgcg aaggttttgc ccctccacaa attccatgac aaggaagtgc
     1501 gtcccgttgt acgaacagac gtcgaacacg ggaacgatat tgggatgccg aagggccatc
     1561 cccaagcgac cttccctcac gaattgctgg taacaattgg ggttgtcact gtagcgccgc
     1621 ctcaagacct tgagggccac aacctccttg gtgtccttgt gaactgctcg gaaaacacgg
     1681 gcgaatgtac ccgctccgac cagataaaga accttataat cgcgataaaa gaaaccgtca
     1741 cgatcaccgt ttaggagttt ttcggcctga tagttggtga ggtattcacg ccgtaccaag
     1801 aattgaatga actcgtccag cgagacgctc tttctgccca actctgacca aatctggcgg
     1861 atctcccaat cggtgaggag accaacatca tgggctcgct ccgccagcac ttctgctgaa
     1921 atgtcggcca taatctctcc ctctgttccg agtattggct caattatggg cgagttaacc
     1981 cgctctgatc agcggcacaa aatctgggta aatgcccctt ccaatgaaat accccgag
//
LOCUS       DAOPTR010000778         2036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_43247_length_2036_cov_3.265018, whole genome shotgun sequence.
ACCESSION   DAOPTR010000778 DAOPTR010000000
VERSION     DAOPTR010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43247_length_2036_cov_3.265018"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1860
                     /locus_tag="PKI05_13095"
     CDS             172..1860
                     /locus_tag="PKI05_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53183.1"
                     /translation="MRRTFFPSALFGLCSTCLLLLGLSSLRSDPRLFAQSQHPDRYSP
                     DSNDSFSEVWFTPDDFEEAYYGESPCVEDDWTFSWLDIDPDAWDTGAVPGDPSVNDPK
                     AGYVTSSEASGYRYYYRFVAEDSWSGDSTSMDQEASEPWLWLSDLPEEPEDLTGLSGY
                     GFIDEYSSEEAARYEMAQEGCWQDDETILAETDGELPWDAESYELLQPQENVGGRGGR
                     CLILNRRTLAGEGSVGAKMITVAELEAELQAERAQAYHQPDYEADVAAGWLFAANRSE
                     DLDDLAFNPSSDGEIDAFEFFEEEIEEIDDEAYEQWEVLEEQDVDAFSTEEINEWDEA
                     DDYLGTTVPETPSAESEPRHDSAATSPEDAEEPYESWIIVNHEPIVGISSGDSEPPAS
                     VGDTPWHGEPGENFVWPMLAEETDMPSPAAEGDLLEEQTGAMDRGDEDQFLWMDPADD
                     RYYDSSFEDNAEQEMLDGTSESGESISGWDVSQDENDPSTGDLGEEWDVGERGYRPAE
                     MAAPVADPFGEHLSPGEDAYEDATNFQGEGGDSVSLKRMGAALVELVSPLRFWH"
BASE COUNT          435 a          534 c          623 g          444 t
ORIGIN      
        1 tctatcttac taattgcgca cattacgaac tcgtcgtgat ttgcccaata tctcacaggt
       61 attcctccga tacgcataac ggcgcatgca tctctgacgc atttttccga cacgccgtat
      121 tttgcccatt cccctaccat ccatcccacc gaaaaaaagg aggagttcgt catgaggaga
      181 accttctttc ccagcgcgct gtttggtctt tgtagcacat gtttgctcct tctcggtttg
      241 tcatcgctcc gctctgatcc ccgactcttc gcccaatcgc agcatcctga tcgctattcc
      301 ccggattcaa acgactcttt cagcgaggtt tggtttaccc cggatgattt tgaggaagcc
      361 tactacggcg agtcgccatg tgttgaggat gactggacat tttcctggct tgatattgac
      421 ccagacgcct gggacaccgg ggcggttcct ggcgacccat cggtcaatga tccaaaggcc
      481 ggatatgtca cgtcgagcga agcctcaggc taccgatact actaccggtt cgtggccgag
      541 gactcctggt cgggcgattc cacttctatg gatcaggaag cgagtgagcc gtggttgtgg
      601 ttaagcgatc tgccggagga acctgaagat ttgaccggcc tgtccggata cggcttcatt
      661 gacgagtact cttccgagga ggccgcccgt tatgaaatgg cccaggaagg gtgctggcaa
      721 gatgatgaaa cgatcctggc cgagacagat ggcgagttgc cgtgggatgc agaatcctat
      781 gaactgcttc agccccaaga gaacgtcggt ggacgcggcg gaaggtgcct tatcttgaac
      841 cggcgaaccc tagcagggga aggctctgtg ggcgcgaaga tgatcacggt ggccgaactt
      901 gaagcggagc tccaggccga gcgggcccag gcataccatc agccggatta cgaggctgac
      961 gtggcggccg gctggctatt cgccgcgaat cgaagcgagg accttgatga tctcgccttc
     1021 aacccatcaa gcgatggcga gattgacgca tttgagtttt ttgaggaaga aattgaagaa
     1081 attgatgacg aagcctatga gcaatgggag gttctcgaag agcaagacgt tgacgctttt
     1141 agcacggaag agataaacga gtgggacgag gcggatgatt acttgggcac gaccgttccc
     1201 gagacgccgt cggccgagtc ggaaccgcgc cacgattcgg cggctacttc tcccgaggat
     1261 gcggaagagc cgtacgaatc atggatcatc gtcaaccacg agccgatcgt agggatttct
     1321 tcgggcgata gcgaaccacc ggcttctgtc ggcgacaccc cttggcacgg cgagcccggc
     1381 gagaatttcg tttggccgat gttggccgag gaaacggata tgccctcacc tgcggccgag
     1441 ggtgatctgc tagaggaaca gaccggtgcg atggatcgcg gtgatgagga tcaattcctg
     1501 tggatggatc ctgcggacga tcgatattac gacagttcgt ttgaggataa tgcagaacaa
     1561 gagatgctcg atgggacgag tgagtcgggc gagtcgatta gcggctggga cgtgtcgcag
     1621 gatgaaaacg atccctcgac cggcgacttg ggggaggagt gggacgtcgg ggagcgaggt
     1681 tatcgtcctg ccgagatggc cgcgccggtt gctgatccct tcggcgaaca tctttctccc
     1741 ggcgaggacg cctatgagga tgccaccaac ttccagggcg agggcgggga ctccgtgtcg
     1801 ctcaaacgga tgggggccgc gctggtggaa ttggtctcac cgctgcgttt ctggcattaa
     1861 actgacgggg cctggtagga tcagcccgcg tcaaacgcgt tcatacgccg tcaaatagac
     1921 cgcgtaaaca cttcctctgg cactggcgac ccccagaaat gggggtcgct ttgtttttgg
     1981 gatggagctg ctctccggtt gaaaattttt ggcggctcgg cgaaaggaga tctctc
//
LOCUS       DAOPTR010000779         2032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_43383_length_2032_cov_2.671725, whole genome shotgun sequence.
ACCESSION   DAOPTR010000779 DAOPTR010000000
VERSION     DAOPTR010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43383_length_2032_cov_2.671725"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            451..1854
                     /locus_tag="PKI05_13100"
     CDS             451..1854
                     /locus_tag="PKI05_13100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOA53184.1"
                     /translation="MATPINDWIDEPGVYRPLLRLAWPVLGEQALAMAVGFSDTILTG
                     HYLGESHLAAVNLMAYTLWLLYGLFSVVAIGTSALVARYVGARRFTMADRFLHQSLIL
                     GLLISVPGAIAALTFGPDLVALLKLRGESAAAAVRYWNIIVPMTPCIMVIAVGIAALR
                     GAGAMVHGLCIMAIVNIVNIAVSWACCLGWGPLPQLGWDGIALGTTCGYLVGAIGTLT
                     ILGSGMIGLRLRWRMFRPEGHLIYRLLRVGIPGGLDSFALILCQLWFVSIVNVLGDRA
                     AAAHGVAIRIESLAFLPGAAFQVAAATMAGQYLGAKQPGKATRSVLVACLLGGLVMGL
                     GGVVFYIGAFPLASLMLGSHHHDVAYLAAPLLRTIAFGIPPLALVMILTGALRGAGDT
                     SWPLVFTLVGFLGVRIPVAYWLALSSVPIPWVNDPLPGLGLGVLGAWYAMVTDLYVRA
                     ILTAWRFWQGGWKEVRV"
BASE COUNT          336 a          604 c          603 g          489 t
ORIGIN      
        1 gcgtttccga aacctcaacc gcctcatgag acacctcctt ccgggaaaag gaataaacca
       61 aacgccacgc ctatcggaca acttgtttag cgccaagcca gttctccctc ctttggcgcc
      121 ccgcattgta atttcgacta gccaaccttg caagcatttt gcgaaatgca tttgagtttt
      181 ttgagcgagc caattttggc caccggcaag ttgccccaaa actcactccc gaaagtcaat
      241 ccggtgttag cagcttggtc tatgcgccgt tcgggccatc taacggcttg gcgacggcga
      301 aaaaccacag ttcccgccga atggggtttc ccgctatact gaaatcttct gcgcctggtc
      361 ggtgccaccg cacccggtct taaaataggt catcgcagca agagatcgaa ccttcgtgtt
      421 gcgccgtctc ccggcgtcgc ccgccttagt atggctaccc ctatcaacga ttggattgac
      481 gaacccgggg tttatcgccc gcttcttcgc ctggcctggc ctgtccttgg cgagcaggcc
      541 ctggccatgg ctgtgggttt ctccgacacg attttgacag ggcattatct tggggaatcc
      601 cacctggccg ccgtcaattt gatggcttac accttgtggc ttttgtatgg actcttcagc
      661 gtggtggcga ttgggacatc cgccctggtg gctcgctatg tgggcgcccg ccgctttacc
      721 atggccgacc gttttcttca ccagtcgtta atcctcggat tgctcatttc cgtcccaggg
      781 gccatcgcgg ccctcacctt tgggccggat ttggtggcac ttcttaaact ccgcggagag
      841 tcggcagccg cagccgttcg ctactggaac atcatcgtcc ccatgacccc ttgcattatg
      901 gttatcgctg tggggatcgc cgcgttgcgc ggggccgggg ccatggtcca tggcctgtgc
      961 atcatggcca tcgtcaacat cgttaacatc gccgtaagtt gggcatgttg cctaggctgg
     1021 ggcccgcttc cccaattggg ctgggatggc atcgcgctgg gcacgacctg cggttatctg
     1081 gtgggggcca tcggcacgct cacgatcctt ggcagtggaa tgatcggcct ccgtctgcga
     1141 tggcgaatgt ttcgtccgga aggccatttg atctaccggc tcttgcgggt gggcatacct
     1201 ggtggactgg attcgttcgc cttgattctc tgtcaactgt ggtttgtctc gatcgtcaat
     1261 gtcttaggag atcgagcagc cgccgcccac ggagtggcca tccgcattga atcgctcgcc
     1321 tttttgccag gtgctgcctt tcaagtcgcc gccgccacca tggccgggca gtatctcggg
     1381 gcgaaacaac cgggcaaggc cacacggagc gtgctggtgg cctgtttgtt gggagggctg
     1441 gtcatggggc ttggaggagt cgtcttctat atcggggctt tccctctggc cagcttgatg
     1501 ttgggatcgc accaccacga cgtggcctat ctggccgctc ctttgttgcg aaccattgcc
     1561 tttggtatcc cgccgctggc cttggtgatg atcctcacgg gggccctccg cggggcgggc
     1621 gacacaagtt ggccgttggt ctttacgctg gtcggcttcc tcggggtccg catcccggtg
     1681 gcatattggc tcgccctctc gagcgtgccc atcccctggg ttaacgaccc cttaccgggc
     1741 ttggggctcg gcgttctcgg cgcatggtac gccatggtga cggacctcta cgtccgcgcg
     1801 atcctgaccg cgtggcgatt ttggcaaggc ggctggaagg aagttcgcgt ctaatccacg
     1861 tttacataaa acccgagaca aacactatac gccgggatcg cgttggtttt tggccatcgg
     1921 ggcaggtaat tagggcgggc acgggatgtg gaatagggca ggcacggggc gggtaattgg
     1981 ggcaggcaca ggggcctgcc cctacggggg atttttccca aacctcggga aa
//
LOCUS       DAOPTR010000780         2031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_43398_length_2031_cov_3.727227, whole genome shotgun sequence.
ACCESSION   DAOPTR010000780 DAOPTR010000000
VERSION     DAOPTR010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43398_length_2031_cov_3.727227"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(458..1717)
                     /locus_tag="PKI05_13105"
     CDS             complement(458..1717)
                     /locus_tag="PKI05_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOA53185.1"
                     /translation="MRPQRWGLFGGCGAFAQEIIGMEAIAQSRFQFDQLVYARDILQC
                     AGQAILRVSRRLGDSFCRAVELLLACRGHVVVSGVGKAGLIGQKISATLASTGTPSHF
                     LHPTEALHGDLGRVRLGDVGLLLSASGETEEMVRLSTALTELQIPIIAMTCSRQSQLA
                     RSAQVVLELGPIEEADHLRLAPSTSTTVMLALGDALALTVSRLRGFRAEDFARFHPGG
                     SLGKKLSRVEEHMRPLSECRLASDQLTLRQVIVATRRPGRRTGAIMLLDAEGKLSGIF
                     TDSDLAKVLERRQDSVLDAPIADLMTRHPITVRAGTSLRDAVRVLAEKKISELPVVNE
                     NGNPVGLLDITDVIALFPDLLSQNGQGTFDERTSTESQQPGDSSEDETPSRPPEPRSN
                     MFRSSQPPGLPELRIVVPEEREYPNER"
     gene            complement(1743..>2031)
                     /locus_tag="PKI05_13110"
     CDS             complement(1743..>2031)
                     /locus_tag="PKI05_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53186.1"
                     /translation="QWPKSDSIYPSLVAVNITRRRFSADCPFGGSSRFSYNPHIAKFR
                     VLPTCYMPPIFSTYTGIVGFQDAVTLLKFSAQATISSDELANRGRISLRNP"
BASE COUNT          455 a          563 c          571 g          442 t
ORIGIN      
        1 gagtcaggtt ccggctcgat cattgggaca gccagccgtg cgtacacctg gtacgccaaa
       61 gcgacgatca ggaagcttac ggctacttgt gcccaacgcc gattcataca ttcaagtcat
      121 tcattggcac cgtgattggg ttatcttgtt agagcctatc ccatgatctc ccctcgcccg
      181 ttcggcggga gagggggcag gggtgaggga ggctcgaatc gctgccggtt ttctcaatcg
      241 attccgacgt tcccttgccc tcaccccaac cctctcccgg agggagaggg gggctttttg
      301 gggtaggctc tctaagtcac ccgccaagta aggacaactc tggtttggga agaggtttcg
      361 actcattcgc taccttttga ggctgtaatc atcaacaccc aaattgcgca gaatgtcatg
      421 tgtcatgtgc cggtctcgta agccggcgaa aaaggtgcta tctctcatta gggtattcgc
      481 gttcctccgg gaccacgatc cgtaactcag gtaatcccgg cggctgactc gagcgaaaca
      541 tgttggatcg cggttcaggg ggccgggacg gcgtttcatc ctcggaagaa tccccgggct
      601 gttgagactc agtcgatgtg cgttcatcaa atgtgccttg cccattttgg gaaagcagat
      661 ccggaaagag ggcaatgacg tccgtgatgt ccaacaagcc aactggattt ccattttcgt
      721 tcacgacggg aagttcactg atcttctttt cggccaagac cctgacggca tcccgaagcg
      781 aagtcccagc acgaacggta atcgggtggc gtgtcatcag atcggcgatc ggtgcgtcga
      841 gaacggagtc ctgccgacgt tccaagacct tggcaaggtc actgtccgta aaaatccccg
      901 atagtttgcc ttcagcgtca agaagcatga ttgcccccgt ccttctaccg ggacgacgtg
      961 tcgcgacgat gacttgccgc agtgttaatt ggtcagaagc taagcggcat tcacttaggg
     1021 gacgcatatg ctcctcgact cggcttagct ttttgccaag gcttccccca ggatggaacc
     1081 gtgcgaagtc ctctgcccga aatccgcgga gacggctgac agtaagagcg agggcgtcac
     1141 ccagggccag catgacggtt gtgctcgtgc tgggggccag tcgcagatgg tcggcttctt
     1201 cgatggggcc cagttcgaga actacttgtg ccgatctggc aagttgactc tgccgggagc
     1261 atgtcatggc gatgattggg atttgcagct cggtaagggc ggtcgagagc ctgaccatct
     1321 cctccgtctc gccacttgcc gagagaagga gcccaacatc gccgagccgg acgcgaccga
     1381 gatccccgtg aagggcctct gtgggatgaa gaaaatggct gggcgttccc gtcgatgcaa
     1441 gcgttgccga gattttttgc ccgatcaggc cggctttacc cactcccgaa accacaacat
     1501 gaccccgaca agccagtaat agctcgacgg ccctacagaa cgagtcgccc agacggcgtg
     1561 ataccctcag aattgcttgt cccgcgcatt ggagaatatc ccgggcatag acaagttggt
     1621 caaattggaa gcgagattgg gcaatggcct ccatgccaat gatctcctga gcaaaagctc
     1681 cacacccgcc aaagagcccc caacgttggg gcctcaaaac ccgacctgcg tccccgaaat
     1741 tattacggat ttcgcagact tattcgtcca cgattcgcca attcgtcgga ggaaattgta
     1801 gcttgggccg aaaacttcag caacgtgact gcatcttgga acccaactat gccggtatat
     1861 gtggaaaaga tgggtggcat gtaacatgtg ggcaagacac gaaacttggc aatatgggga
     1921 ttgtacgaga atcgcgacga gcctccaaaa gggcaatcgg ccgaaaatct cctccgcgtg
     1981 atgttcactg ccactaagga cggataaatg ctgtcacttt tcggccactg c
//
LOCUS       DAOPTR010000781         2030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_43428_length_2030_cov_3.752405, whole genome shotgun sequence.
ACCESSION   DAOPTR010000781 DAOPTR010000000
VERSION     DAOPTR010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43428_length_2030_cov_3.752405"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..823
                     /locus_tag="PKI05_13115"
     CDS             158..823
                     /locus_tag="PKI05_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008670103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HOA53187.1"
                     /translation="MSVAAPIDRGQVEKIVREVVIKLLGGPAERPEVVVSVSARHCHL
                     SREHIDILFGPGYELKPYKWLYQEGFYAAEETVMIVGPRRRMLPTLRVLGPPRSKTQV
                     ELAFTDAISLGIDAPVRLSGDLKDTPGCVLIGPEGSVDLKEGVIRAARHVHMNVRDAA
                     FYGVKNGDVMSLRVESGPCTTVFEGLVVRADEQSKLEVHLDTDEGNAADIDHATRVEL
                     FKP"
     gene            932..1225
                     /locus_tag="PKI05_13120"
     CDS             932..1225
                     /locus_tag="PKI05_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOA53188.1"
                     /translation="MEALGMLETKGFVTLVEATDAMLKAANVQFLGWDKVGSGLVSVF
                     VTGDVAAVKAATDAGAAAASRIGEVVSVQVIPRPHDDLNVVLPTAPAKGGKTD"
     gene            1298..1567
                     /locus_tag="PKI05_13125"
     CDS             1298..1567
                     /locus_tag="PKI05_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOA53189.1"
                     /translation="MQDAIGLIETRGLVALVQATDAMAKAANVQIVKRVQIGGGLVTT
                     IVRGDVGSVRAAVEAGANAAAQVGELVASHIIPRPAEGVIAAYLT"
     gene            1750..>2030
                     /locus_tag="PKI05_13130"
     CDS             1750..>2030
                     /locus_tag="PKI05_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007333580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HOA53190.1"
                     /translation="MHILVANLGSTSFKYRLFDMTTERQLARGGVERIGAPESRCFVE
                     IQGQRHEESRHVPDHAEAVRQCLAQLTDPQRGCLKDAREVAAIGFKAVHG"
BASE COUNT          447 a          489 c          670 g          424 t
ORIGIN      
        1 cgcctgattg tggccccact ggctgaagag gcagcgcggg gtgggacgac aaatccgcca
       61 gattgatcgt gtctgtgatg gagggattgg cgacagaaaa gcggcctgag ttggccggga
      121 aattttaaac aacacgagac ggaagggcat tcgatccatg agtgttgcgg caccgataga
      181 tcgcggtcaa gtggaaaaga tcgtccgtga ggtcgtcatc aagttattgg gagggcccgc
      241 ggaacgtccg gaagtcgtgg tcagtgtctc agcgcgtcat tgtcacctat ctcgggagca
      301 tatcgacatc ctttttgggc ctggctatga gcttaagcct tacaaatggt tgtaccaaga
      361 agggttctac gccgccgagg aaacggtcat gattgtgggt ccgcggcggc ggatgttgcc
      421 aacgcttcgt gtgctggggc cgccacggtc aaagacgcag gtcgagttgg cctttacgga
      481 tgccatttct cttggaatcg atgccccagt tcgtctgagt ggtgatctga aggatacacc
      541 cggatgtgtg ttgattggtc cggaaggaag cgtggacttg aaggagggcg tgattcgcgc
      601 ggccaggcat gtccacatga acgtgcggga tgcggccttc tacggcgtga agaatggcga
      661 cgtcatgtcg ctacgtgtgg agtcgggacc ttgcacgacg gtgttcgaag gcttggttgt
      721 gcgggccgac gagcagagca agcttgaggt ccatctcgac acggatgaag gaaatgcggc
      781 cgacatcgat cacgccaccc gcgtggagtt gttcaaaccg taaccggagg aaataaagtc
      841 aacaaagtca agaggttcgt cagagtcgtc aatctgatga tgtaaatgat cggcaccttt
      901 tcgtgtggag tgaacgccca tgaccaaaca gatggaagcc ctgggaatgt tggaaacgaa
      961 aggttttgtg acgctcgtgg aagcgaccga tgccatgctc aaggctgcca atgtgcagtt
     1021 cttgggttgg gacaaagtcg gcagtggatt agtcagcgtc ttcgtaaccg gtgatgttgc
     1081 ggccgtcaaa gccgcgaccg atgcgggagc cgctgccgcc agccggattg gtgaggtcgt
     1141 aagtgtgcaa gtcattcctc gacctcacga cgacctgaat gtggtgctcc caacagcacc
     1201 ggcaaaaggt ggaaaaaccg actgagaccg tgttatgcct cggaaacaga ctcaaaaaag
     1261 aaacttggcc aacgatgaaa tagggggatt tcgcactatg caagacgcca tcggattgat
     1321 cgaaacgcgt ggattagttg ctttggttca ggctaccgat gccatggcaa aagccgccaa
     1381 tgtgcaaatc gtgaagcgag tgcagattgg cggcggtctg gtgacgacga tcgttcgtgg
     1441 ggacgtgggg agcgtccgtg ccgcggttga agccggtgct aacgctgccg cccaggtcgg
     1501 tgaattggtg gccagtcaca tcattccgcg gccagccgag ggtgtcatcg cggcctactt
     1561 gacgtgattc cctaggccat cttgtgtgtc ggggccgaca ccttccgccg aaccagattc
     1621 ggtgtgaaca agtgttcggt gatccggcaa gaagatgccg cctcgggttt cgcggtcagc
     1681 atgacatcag cggatggcag tgatccgcag tatgggatga cgggacaaga tttggaagga
     1741 ggtcgcagga tgcatatctt agtggccaat ctcggaagca ctagtttcaa gtaccgactg
     1801 ttcgacatga ccacggagcg ccagttggct cgtggtggtg ttgagcggat cggtgctccg
     1861 gaaagccgtt gctttgtgga aatccaaggg cagcggcacg aagagtcccg gcatgttccg
     1921 gaccacgccg aggcggtgcg ccagtgcctt gcccagttga ctgatccgca gcgagggtgc
     1981 ctcaaggatg cccgggaggt agctgccatc ggtttcaagg cggtgcacgg
//
LOCUS       DAOPTR010000782         2026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_43562_length_2026_cov_2.755961, whole genome shotgun sequence.
ACCESSION   DAOPTR010000782 DAOPTR010000000
VERSION     DAOPTR010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43562_length_2026_cov_2.755961"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            540..1463
                     /locus_tag="PKI05_13135"
     CDS             540..1463
                     /locus_tag="PKI05_13135"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOA53191.1"
                     /translation="MELQLIDYFRQRIPQYPCVRLGIGDDAAIIDLDIPQCVVTTDIL
                     MDGVDFRLSEVTPEAIGRKALAVNLSDLAAMAASPKACVVGLVLPEKGGVEIAKRLYD
                     GLLPLARDFQIAVAGGDTNSWEGPLVISVTAIGLPSPGGGLCRAGAKPGDRIIVTGDF
                     GGSLLGKHLTFTPRVWEAIYLYRSYEIHAGIDVSDGLALDLWRLTVESRCGAVIQAEK
                     VPISEASHRMVAQVKSRMKPLDHALHDGEDFELILAVPPAEAARMLRDQPLSVPLTDI
                     GEFVPDRGLWLEKGKKRTPLEPKGYQHKFSL"
     gene            1676..>2026
                     /gene="tsaE"
                     /locus_tag="PKI05_13140"
     CDS             1676..>2026
                     /gene="tsaE"
                     /locus_tag="PKI05_13140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOA53192.1"
                     /translation="MITRSEEETRQLGALIAQCLPRPSVIGLMGTLGSGKTRLVQGIA
                     HGCGVREAAVISPTFVLIHEYPGDVPVFHFDVYRLKDVAEFLDLGPEEYFQRPGISVI
                     EWADRVEEYLPPHRL"
BASE COUNT          404 a          510 c          604 g          508 t
ORIGIN      
        1 catgcgtagg acagtccccg gttccagttt ttgctcttca ataattcgtt tgatttctga
       61 cgccgcgact ggggttaagc ttaatcccat agactcgtga tcctccaaca ctaaattctt
      121 tctaacagac gtttcgagca cgccgagttg accggttttg gaattgttcc aatctttgat
      181 gaatcatacg caaatcgtgg cgattttcca aggatgtgat ccacaaaggg gagagcacga
      241 gtggcgatgg cttggcagcg gagaggtcta gtctcttgga aggcggggat gctgggtttc
      301 caacctacga ttgctgcccc gctaaaaccg tgtgatctcc aatgaccggc gaacccggca
      361 aaatggcgat cccctgtttc ccctggtggt ttcttcgcgg taagcttaaa caaagagaac
      421 cggttgacgg tattcgacgg ctgattaacg tcgagtgggg acgcggttta tttttggttc
      481 tctcctcgaa tgttcgtgcg cgcacctgtt gcgtgctttg agtttttgga gtcgatctca
      541 tggaactcca gcttatcgat tacttccggc agagaatacc ccaatacccc tgtgtccgct
      601 tggggattgg cgacgatgct gcgattattg acctcgatat accccaatgc gttgtgacca
      661 cggacatcct gatggatggc gtggatttcc ggctgagcga ggtaaccccg gaggccattg
      721 ggcggaaggc ccttgcggtc aacctgagtg acctggcggc catggcggcc tcgcccaagg
      781 cgtgtgtcgt aggacttgtg cttcccgaga aaggtggggt cgagatcgcc aagcggctct
      841 atgatggttt gttgcccctg gcccgtgatt ttcaaattgc tgttgctggg ggagacacca
      901 atagttggga ggggccactg gtcattagtg ttacggctat cggtttaccc tctcccggcg
      961 gggggctttg ccgtgccggt gcgaagcccg gggatcggat tatcgtcacc ggtgactttg
     1021 gcggatccct tctgggcaaa cacctcacat tcacgcctcg ggtttgggag gccatttatc
     1081 tctaccgttc ctatgaaata cacgcaggaa tcgacgtgag tgacggattg gcgctggatc
     1141 tctggcgact gaccgtggaa agccgctgtg gtgccgtcat tcaggcggag aaggttccca
     1201 tttcggaggc ttctcatcgc atggtggccc aggtcaagtc ccgaatgaag cctttggatc
     1261 atgccctcca tgatggtgaa gattttgagc tgatcttggc cgtgcctccg gccgaggcgg
     1321 ctcggatgct ccgcgatcaa ccgttgtcag tcccgttgac cgacattggc gaatttgtcc
     1381 cggatcgagg tctttggctg gaaaagggaa aaaaacggac cccactggaa ccgaagggat
     1441 accaacacaa gttttccctg tagaaaagag agctttgaac ggcatgccca aagtacgggt
     1501 cgtcttctat cgactgaaac gttttcgggt cttagccttt tattgggcgt atgactgatc
     1561 ggttagcgta tggcgtcgcg cccgtacgat ggagggctcg ctggtgcgca ggagtgcaaa
     1621 tgaagttctt tccttattgg tgaggtgaaa ccgagccgtg gatgcgcgtg gaaacgtgat
     1681 aacacgttct gaagaagaaa cacgacagct gggagcgttg attgcccaat gtttgccccg
     1741 gccgtctgtg atcgggctta tggggacgct tggctcaggc aagacccggc tggtccaggg
     1801 gattgcccac ggctgcggag tccgcgaagc ggcggtgatc agtccaacgt ttgtcttgat
     1861 ccacgaatat ccgggtgacg ttcccgtctt ccattttgac gtgtaccgcc taaaagatgt
     1921 ggcggagttt ctcgaccttg ggccggaaga gtactttcaa cgtccgggaa tttctgtcat
     1981 cgaatgggcg gaccgtgtcg aagaatatct cccgccgcat cgcttg
//
LOCUS       DAOPTR010000783         2021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_43697_length_2021_cov_3.535097, whole genome shotgun sequence.
ACCESSION   DAOPTR010000783 DAOPTR010000000
VERSION     DAOPTR010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43697_length_2021_cov_3.535097"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PKI05_13145"
     CDS             <1..284
                     /locus_tag="PKI05_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53193.1"
                     /translation="PAEGWRFQPCFCSVGDSRNIQTGGWFPPDLCLGAKPQVDDVLSF
                     AEASRDYDLEGDPKRPLTLPEKYALKDYSHYTSMLLSIQHTILSAGSFL"
     gene            828..>2021
                     /locus_tag="PKI05_13150"
     CDS             828..>2021
                     /locus_tag="PKI05_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53194.1"
                     /translation="MARKKPRSPGANGQEETAEMPPAPEQTPAPQTRADQVGSAVEMS
                     PCREPEQQDAGHTVHADRPVASNDVTRSSAPKPPTAPQRAEAAAMDMLRTADLKELVK
                     QIQADIAAVGVAGEESMSLLIYLVGTSRLLDHPLAAVVQGPSAAGKSYVIATVSKLFP
                     KDCVVQAHRITPQALVDVPAGELVNTFVVGGERSRRRDPEATRTLRELLADGHTSVLR
                     PKRKTDSTGRKDVKGPIAFIESTTLEPDEIFGEDLNRCLLVHVDASAAQTKRVLIHMA
                     ERAKSPNSTDVTPITAKHHAAQKLLKKCNVFVPYAETLAKLYPYETVEARRSFPLLLS
                     LIRTIRLLRQYQQSMEPEDGMPLAADLDDYELARYLLARPKNSGLTGKIAETYQMLPK
                     LRPRFS"
BASE COUNT          453 a          619 c          563 g          386 t
ORIGIN      
        1 cgccggcgga gggctggcgt tttcagccct gcttctgcag cgtaggcgat tcccgcaaca
       61 tccagacggg cggctggttc ccgcccgacc tatgccttgg ggccaagccc caggttgatg
      121 acgtgttgtc gtttgccgaa gcatcgaggg attatgacct tgaaggtgat ccgaaacgtc
      181 cgctgaccct gccggagaaa tacgcgttaa aagactattc gcattatact tcaatgttgt
      241 tatcaataca acacacaata ctctcggcag gatctttctt gtaattagcg cgattccggg
      301 acgattctct tgttctgggg ggcattattc acaaatactg ctaaatgctc gcaacggatc
      361 gcgacccttg cattgtcggg catgcaatta gctgatcacg attaattact ctgtccggcg
      421 aaattggcgc aaacaaggaa acgggcgccg gaattagccg gagatcacgg cgctcgccgg
      481 gtcgagcccg tcagccacta ccccttggac tcggtttaac ctgtggaaga gcacggccct
      541 cgctgtttgg tggactccct tctcccgcca tgaactctcc ggaaagcatc caggtttcga
      601 gaactcatcc tgcccgtgcg gcaaccatta aggatgtgct ggtcccaaca tgccgttgaa
      661 tcaaccgcgg caacacataa ccacctgaaa taaaggcagt taggataact gcgtaggtgg
      721 ctaccccaaa tcctaaacac catttcaaag ccgggttcta accgctatca tggcgccctc
      781 gctggcggcc cgatgggatc ggccagccgg caaacagaag gacttgcatg gccagaaaga
      841 aaccacggtc gcctggggca aatgggcagg aagagaccgc cgaaatgccg ccggcacccg
      901 agcagacccc tgcccctcag acccgcgccg accaggttgg ctcggcggtt gagatgtcgc
      961 cttgccgcga gcccgagcag caggatgcag gacacaccgt acacgcggac cggcctgtcg
     1021 cctcgaatga tgtgacccga tcgagtgccc ctaaacctcc cacagctccg caacgtgccg
     1081 aagctgccgc aatggacatg ctgcgaacgg cggacctcaa ggaactcgtc aaacaaatcc
     1141 aggcggacat tgccgcggtc ggggtcgccg gcgaggaaag catgtcgctg cttatttacc
     1201 tggtcggcac ctcacgactg ctggatcatc ccctggccgc ggtcgtgcaa ggtccgtccg
     1261 cagccggaaa gagctacgtg atagccaccg tatccaagct gtttcccaag gactgcgtcg
     1321 ttcaggccca tcggataacg ccgcaggcgc tggtcgacgt tcccgcgggg gaactcgtga
     1381 acacgttcgt cgtagggggc gagcggtccc gccgccgtga tcccgaagcg acacgcacgc
     1441 ttcgcgagtt gctcgccgac gggcacacca gcgtgctccg tcccaagcgt aaaacggatt
     1501 cgaccgggcg aaaggatgtg aaaggtccaa tcgcgttcat cgaatccacg acgctggagc
     1561 cggacgaaat cttcggcgag gacctgaatc gctgcttgtt ggtgcatgtg gacgccagtg
     1621 ctgctcagac caaacgtgtg ctgatccaca tggccgaacg tgccaaaagc ccaaattcaa
     1681 ccgacgtgac cccgatcacc gccaagcacc atgctgcgca gaaactgctc aagaagtgca
     1741 atgtctttgt accctacgcg gaaacgctgg ccaagctcta tccctacgaa accgtagaag
     1801 ctcggcgatc tttccctctc ttactctcgc tgatccgaac gatcaggttg ctgcggcagt
     1861 accagcaatc gatggagcct gaggatggaa tgccgctggc cgccgacctg gatgattacg
     1921 agcttgcccg ttatctgctg gcgagaccca agaactctgg gctgactggc aaaatcgctg
     1981 agacgtacca aatgctcccg aagctaaggc cgcgattttc c
//
LOCUS       DAOPTR010000784         2017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_43835_length_2017_cov_3.449032, whole genome shotgun sequence.
ACCESSION   DAOPTR010000784 DAOPTR010000000
VERSION     DAOPTR010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43835_length_2017_cov_3.449032"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PKI05_13155"
     CDS             <1..709
                     /locus_tag="PKI05_13155"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA53195.1"
                     /translation="GNLRKSLRELGIHENTLLWYTSDNGAIEVGSTGGLRGRKGTLYE
                     GGIRVPCVIEWPAKIKTPRRSDFPCGTVDIYPTLLDIVGITIANQPPLDGISLVPLLE
                     GREPPRRDKPLGFWVFPVGGISTPSEKILAQMLEEQEGRASASPPPKDEDKITQQYSE
                     DQLLGHAAWLDWPFKLHRIAGKGGAVKWELYNLATDVTESTDLASQEPERVQTMGAAL
                     EAWQKSVLRSLNGEDYR"
     gene            937..1947
                     /locus_tag="PKI05_13160"
     CDS             937..1947
                     /locus_tag="PKI05_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53196.1"
                     /translation="MSERGDRRDFLRRGLVGAAGVTAFGLEEKILLAALQQGANQQPA
                     TQDDRPMPFGQIGNLKISRMLIGGNLIGGWAHSRDLLYVSRLFKAYNTDEKVFETFAL
                     CEAHGINTVQLDPRCMDVFHRYLTERGGKMQALVCVNPDADTGKLKSQMESLLSQGAT
                     FFYTHGGATDQQMMNGRLDVVAKTVEAIKEMGVPGGVGSHSLSVPKACEENGIGADFY
                     VKTLHSDNYWSATPEELREDFGWFKPRRDEPFSFNDNMWCIDPEETIEFMKSVRKPWF
                     AFKVLAAGAIHPRMAFPYVLRNGADFVIVGMFDFQIAEDVAFFKDAYYKLGERRERPW
                     CA"
BASE COUNT          452 a          497 c          619 g          449 t
ORIGIN      
        1 gggtaatttg cggaagtctc tccgggagtt gggaattcac gagaatacgc tcttgtggta
       61 caccagtgat aacggcgcga ttgaggtagg ctccacgggg ggccttcgtg ggcgaaaggg
      121 cacgctttat gaaggcggaa tccgtgtccc ctgtgttatc gaatggccgg caaagatcaa
      181 aaccccgcga cgcagtgact tcccttgtgg cacggtggat atctatccca cacttttgga
      241 cattgtgggc atcacaatcg ccaatcagcc gccactcgat ggaatcagct tggtcccctt
      301 gcttgaaggt cgggagccac cccgacggga caagccgctg ggcttttggg ttttccctgt
      361 cggggggatt agcacgccga gtgaaaagat ccttgcccaa atgttggagg agcaagaggg
      421 aagggcttcg gcatctcccc caccgaagga tgaggataaa attacccaac aatattcaga
      481 ggaccagctc ctcggtcatg cggcatggtt ggactggccc ttcaagctac accggattgc
      541 gggcaagggc ggagctgtca aatgggagtt atacaacctg gccacggacg tgacagagtc
      601 gacagacctg gcctcgcaag agcctgagcg cgttcagaca atgggggccg cgttggaggc
      661 atggcaaaag tcggtccttc gcagtttgaa tggcgaagac tatcggtaaa caacaaggtt
      721 tttagcagtg gtgggaggct ccacgcaaag atgacccacc atcgatgggt gtcccggaga
      781 tacggatcac acgtggcgag gatggtcgaa ccttgtgaca ttgcggtcgg gcatgacctg
      841 tcctaaaatt gggcagcatc agtcgagaag gcgttatatc gagttggcga ttgttcctct
      901 caaagacggt gattcatagt tgaaaggaaa cgagccatgt cagagcgggg tgacaggcga
      961 gattttctcc ggcgcggtct tgtcggtgcg gcgggtgtga cggcgttcgg tcttgaggag
     1021 aaaatccttc tggctgctct tcaacaaggg gccaatcagc aacccgcgac tcaagatgac
     1081 cgaccaatgc cgtttgggca aatcggcaat cttaagatca gccggatgtt gatcggtgga
     1141 aaccttattg ggggctgggc gcacagccgt gatcttctct acgtttcacg gctcttcaaa
     1201 gcctacaaca ccgatgaaaa agtgttcgaa acgttcgccc tttgcgaggc gcatgggatc
     1261 aacactgttc agctcgaccc aaggtgcatg gatgtcttcc atcgatatct cacagagcgg
     1321 ggaggcaaga tgcaggccct tgtgtgtgtt aatccggatg ctgatacggg taagcttaag
     1381 tcccaaatgg agtcgctact gagtcagggt gccacgttct tttacaccca cggtggcgcc
     1441 acggaccagc agatgatgaa tggccgtctt gacgtagtgg ctaaaacggt cgaggctatc
     1501 aaggagatgg gggtacctgg cggtgttggc agccattcct tgagtgtccc caaggcttgt
     1561 gaagaaaatg gtatcggggc cgacttttac gttaagacct tgcacagcga caactattgg
     1621 tcggccacac ccgaggagct tcgggaagat ttcggctggt tcaaaccccg gcgtgatgag
     1681 ccattctctt tcaacgacaa catgtggtgc atcgatccgg aagagacgat cgagttcatg
     1741 aaatcggttc gtaagccgtg gtttgccttc aaggtgctgg ctgccggagc gattcaccca
     1801 cggatggcat tcccttatgt cttacggaac ggggccgatt tcgtgatcgt cgggatgttc
     1861 gattttcaaa ttgctgagga tgtggccttt ttcaaggacg cctactataa gctcggtgag
     1921 cgccgtgagc gaccctggtg tgcttaagat ttatcccaca agctcccctc gcccgttcgg
     1981 cgggagaggg gccgggggtg agggtggctc gaatcgc
//
LOCUS       DAOPTR010000785         2017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_43840_length_2017_cov_2.695209, whole genome shotgun sequence.
ACCESSION   DAOPTR010000785 DAOPTR010000000
VERSION     DAOPTR010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43840_length_2017_cov_2.695209"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PKI05_13165"
     CDS             complement(<1..519)
                     /locus_tag="PKI05_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53197.1"
                     /translation="MIGKKELKPNDVEVISSEGEIRVGMKDGVRYLLRFGQVVAGGSS
                     GQEGEGGAGVNRYLFVMAEFDPDLIPKPELQPLPEVPSEGQSPAATTGTSQSQSAATE
                     EAQSGTSVTQGSAPPEQSSGSEEAKSQTSEEKPAEQQKKPEDIKAERERIEKENKRKQ
                     DEYEEKLKKGQER"
     gene            complement(562..1524)
                     /locus_tag="PKI05_13170"
     CDS             complement(562..1524)
                     /locus_tag="PKI05_13170"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOA53198.1"
                     /translation="MNETKKTFAFVAVAAVVVLIAWWTRYTPPVTTTADMRGKPLFPG
                     FTDALAAKSLEIFEYDPTSVKIKNFKVAQINNRWSIPSHENYPADAKDHLAQAATSLI
                     GLTILDVASESPTQEEVALYGVVEPDEKIIKTSVRGIGKRVIFRDQGDKVLADLIIGN
                     KVPDKEELRYVRIKGQDPIYVVKLSTDKFSSEFGDWIEKDLLKLNPWDIKDVQLHDYS
                     FDSLTQTLAPRSQVILGYDDLGSPRWKLVQNLVFDGDQGTWKPQSLAEDEELDTSKLD
                     SMRTALDDLKIVDVRRKPEGISASLSADGTLAANRETAASLAQA"
     gene            complement(1687..>2017)
                     /locus_tag="PKI05_13175"
     CDS             complement(1687..>2017)
                     /locus_tag="PKI05_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53199.1"
                     /translation="QSIKKLEEDMKKQNISLDEILRRVAIAQEQGQRRLDARMEEERQ
                     RRDRALERIETELTLYVRRLQNFYKMVSVIIPPLFPLGLAVIVFVWRRARELEGVPTS
                     RRVRKRF"
BASE COUNT          386 a          590 c          495 g          546 t
ORIGIN      
        1 ccgctcttgc cccttcttaa gtttttcttc gtattcatct tgctttcgct tgttttcctt
       61 ttcgatccgt tcccgctcgg ccttgatatc ttccggtttt ttctgctgct ctgccggttt
      121 ctcttctgat gtttgggatt tggcttcctc tgaacctgaa gactgttccg gcggtgccga
      181 gccttgcgtg acggatgtcc ccgattgggc ttcttcagtg gccgccgatt gactctggga
      241 tgtgcctgtc gtggctgctg gggattgccc ctcggaaggc acctccggga gcggttgtaa
      301 ctcaggcttg gggatcaagt ccggatcaaa ctctgccatg acaaagagat accggttgac
      361 gcctgcaccg ccttccccct cctgtccgga cgatccaccg gcaacaactt gaccaaatcg
      421 caagagatac cgaaccccgt ccttcatgcc cacccgaatc tcgccttcac tcgagatgac
      481 ttccacatcg ttgggtttca gctccttttt accgatcatg gggaagatct tgtaaaagtc
      541 cctggccgaa acaagataga atcaggcctg ggctagcgac gccgccgtct cgcgattggc
      601 agccaacgtt ccgtcggcac tcagcgatgc gctgattccc tcgggttttc ggcggacatc
      661 gacgatcttc aggtcgtcca acgccgttcg catcgagtcc agcttgcttg tgtcaagctc
      721 ttcgtcctcc gccagtgact gcggtttcca cgtcccctga tcaccatcga agaccaggtt
      781 ttggacgagc ttccagcgcg ggcttccgag atcgtcataa cccaatatga cttgcgatcg
      841 cggggccaac gtctgtgtta aggagtcaaa cgagtaatca tggagctgca catccttgat
      901 atcccaggga ttaagtttca gaagatcctt ttcgatccag tcgccgaatt cgctagaaaa
      961 cttatccgtg gacaatttca ccacataaat tgggtcttgt cctttgatgc ggacgtatcg
     1021 cagctcttct ttgtcaggaa ccttgtttcc gatgattagg tccgccaata ctttgtcacc
     1081 ctggtcacga aagatcaccc tttttccgat gccacggaca ctggtcttga taatcttttc
     1141 atccggctca acgaccccat agagtgcaac ctcctcctgg gttggtgatt cactggcgac
     1201 atccaggatg gtcaagccga tcaggcttgt ggcggcttgt gccaggtgat ccttggcatc
     1261 cgccggatag ttctcatgac tagggatgct ccagcgatta ttaatttgag ccaccttgaa
     1321 attcttgatc tttacgctcg tgggatcata ttcaaagatt tccaagcttt tggcggccag
     1381 ggcgtccgta aagcccggaa agagcggctt ccctcgcata tcagcagtcg tcgtgacagg
     1441 cggggtgtag cgcgtccacc aggcgatgag gacaaccact gcggccacgg caacaaaggc
     1501 aaacgtcttt ttggtttcgt tcatcgttgt ctatctcctc gcttcacgga tgggcattgg
     1561 ctgatttccc gtctatcttg ggcccaacca cgaacacgat tgcttatcct aatctgattt
     1621 cctcatccac ccgtcgtttt gccgggtctg acggcttctc gggagttcag tacgatactt
     1681 cacttctcaa aaccttttgc ggacacggcg ggacgtcggg acaccttcca gctcccgtgc
     1741 ccttcgccac acaaagacga tcaccgcaag tcccaacggg aacaacggcg gaatgattac
     1801 cgagaccatc ttatagaagt tctggagtcg ccggacgtac aaggtcaact cggtctcaat
     1861 tcgctccagt gctcggtcgc gacgctgccg ttcttcctcc attctggcgt cgagccgtcg
     1921 ttgaccttgt tcctgcgcaa tggcaacgcg gcggaggatt tcgtccaaac tgatattctg
     1981 ctttttcatg tcctcttcga gctttttgat actctgt
//
LOCUS       DAOPTR010000786         2012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_43975_length_2012_cov_3.388350, whole genome shotgun sequence.
ACCESSION   DAOPTR010000786 DAOPTR010000000
VERSION     DAOPTR010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43975_length_2012_cov_3.388350"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..1278
                     /locus_tag="PKI05_13180"
     CDS             268..1278
                     /locus_tag="PKI05_13180"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOA53200.1"
                     /translation="MKTIGVDSAKGHRVLAALAASCFVWGLLADVTRAQGTNGAEGPF
                     TVEKTEHGVSVKYDGKLWANYVADGEFKPYVWPIIGPTDQPVTREWPMGEGLNERHDH
                     PHQKSMWFTHGDVNKTDFWAEGPNRGRIVHREFVQVSGGKNAVIVTRNDWLLPDGTKT
                     LEDTRKLIFSRSGDHRIIDFVIRLTATADSVTFGDTKEGTFGLRVAESMAVDSKKGGQ
                     IINSEGQTDAQAWGKKAKWVDYSGPVGDQIVGIAVLNHPTSFRFPTHWHVRTYGLFAA
                     NPFGWHDFVGRSDVSGAYTLGKGQSITFAYRIILHKGRAEDARIAQAFEEYAAQPVGD
                     LP"
     gene            1657..>2012
                     /locus_tag="PKI05_13185"
     CDS             1657..>2012
                     /locus_tag="PKI05_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA53201.1"
                     /translation="MEWTIGLGSISLFFGRTLRWLVTKRPRRETILWNFYQIGVLSLP
                     VVALTGTFVGMVLAVQSYAQFKMLNLETRLGAVINMSLVRELGPVLAATMLAGRVGSA
                     MAAELGTMRVTEQIDAL"
BASE COUNT          448 a          512 c          621 g          431 t
ORIGIN      
        1 aagtccccct ctccctccgg gagagggttg gggtgagggc aagcgaacgt cgggctcgat
       61 tgagaaaacc ggcagcgatt caagtcaccc tcaccccgga cacctctccc gccgaacgag
      121 agaggggaga ttatgggata ggccctaagg cgatctgcgc ccagaagatg ctgaggtagt
      181 ggcaaacaca atgcccgacg attaaactgg gcaggtaatt aatgtgtaaa ctgacttgcg
      241 agtcaccgaa tgtgaggaga catcactgtg aagacgattg gtgttgattc ggcgaaaggc
      301 catcgagtgc tcgctgcttt ggccgcgagt tgcttcgtat ggggtctctt ggcagatgtg
      361 acgcgtgctc agggaacaaa tggggccgag gggcctttta cagtggagaa gacagagcat
      421 ggtgtgagcg tcaaatacga cggcaagctt tgggcaaact acgtggccga cggagaattt
      481 aaaccttatg tctggcctat cattggtccc acggaccaac cggtcacgcg ggaatggccc
      541 atgggagaag gcctcaatga acgtcacgac catccccatc aaaaatccat gtggtttacc
      601 cacggcgatg tgaacaagac cgacttttgg gcggaagggc ctaaccgtgg tcggatcgtc
      661 catcgcgagt tcgtccaggt ctctggggga aagaacgctg tcatcgtcac ccgaaatgat
      721 tggctgctcc ccgacgggac gaaaaccctc gaggataccc gcaagctcat cttctctcgt
      781 tccggcgacc accgcatcat cgatttcgtc atccgtttga cggcgacggc cgacagtgtc
      841 accttcggcg ataccaaaga ggggaccttt ggcctgcggg tggccgagag catggcggtg
      901 gatagcaaaa aaggtggcca aattattaat agtgaaggcc agactgacgc gcaggcttgg
      961 gggaaaaagg caaaatgggt tgactattcc gggcctgttg gagatcagat tgtgggaatt
     1021 gccgtgttaa atcatcccac cagtttccga ttcccaacgc actggcatgt taggacctat
     1081 ggtttgtttg cggcaaatcc atttggttgg cacgatttcg tgggccgctc cgatgtaagt
     1141 ggcgcctaca ccctcggcaa aggccagtcg attacgttcg cctaccggat tattctccac
     1201 aaaggccggg ctgaagatgc ccgaatcgcc caggccttcg aggagtacgc cgcccaaccg
     1261 gtgggtgatc tgccttaaaa gacccttttc ggcgcgtgag tcaccagtgg cccgcgacgg
     1321 attcgtgggg cggggagtat gcgcttgcca agaacctgcc gtatcttgcc caatttcttt
     1381 agccttgcca gtcgtttcaa gttgtagaac tgttacgatt gattgaaggc cggtcgggac
     1441 gggcgtcgaa gtactagatc agggatgttg cgccccacgg aggggctgcg cagacgtttc
     1501 gcctgaaaat agagatgaga aaaggcccag cctttccggc ttgggaaaga gacaatgagc
     1561 caaattgtgg caaagtcccc caacggatta ccgggcgtac ccggcgggtt gctcaataat
     1621 tttgcccagt gggtggccga tatcggtgcc cgcacgatgg agtggactat cggcctgggt
     1681 agcatcagcc tgttttttgg gcggacactt cgctggctgg tgacaaaacg tccgcgccga
     1741 gagacgatcc tttggaactt ctatcagatt ggggtcctca gcctgccggt ggtggcccta
     1801 acagggacgt tcgtaggtat ggtcctggcc gttcaaagtt acgcccaatt caaaatgcta
     1861 aacttggaga cgcggcttgg ggccgttatc aacatgtcgc tggttcgtga gcttggccct
     1921 gtgcttgcgg ccacgatgtt ggcgggccgg gtaggaagtg ctatggccgc agagttggga
     1981 acgatgcggg tgacggaaca gattgatgcc ct
//
LOCUS       DAOPTR010000787         2003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_44244_length_2003_cov_2.649897, whole genome shotgun sequence.
ACCESSION   DAOPTR010000787 DAOPTR010000000
VERSION     DAOPTR010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44244_length_2003_cov_2.649897"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(369..1031)
                     /locus_tag="PKI05_13190"
     CDS             complement(369..1031)
                     /locus_tag="PKI05_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53202.1"
                     /translation="MKAPLGQVDNPFSARFVRPGVIPYIFPPGCSWESVHEIFQANQR
                     LGAVIGPHGAGKSAFITAFTHWMENNGQPCLALELHDGQRTLGQATWRKLEALQDTVV
                     TVDGYEQLSAWSRYQLRRLCRKKHLGLIVTAHTDIEIPLLFAPEPNEDLAWQIVQYLL
                     ARGQPLINRDDVRASFARHQGNLREMLFDLYDLYEVRRTSEADSLGALQASKSSSEQD
                     DS"
     gene            complement(1028..1429)
                     /locus_tag="PKI05_13195"
     CDS             complement(1028..1429)
                     /locus_tag="PKI05_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53203.1"
                     /translation="MTEPRILIHLDGKKTVYEPGEVLAGQVRIEPLPLQDIKAVELSI
                     LWYTDGKGDQDLHVRYFRRWSQEGDLPLDLREPLRFQTVLPQTPLSYDGLIIRIRWCV
                     RVRVFRARGRELVEEVPFRLGRVAPARVRLR"
     gene            complement(1426..>2003)
                     /locus_tag="PKI05_13200"
     CDS             complement(1426..>2003)
                     /locus_tag="PKI05_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53204.1"
                     /translation="LPCLVIGLTLLGAFFRQWMATSAVGETIVEISDFPLLCGHRYRV
                     YLKQTGWLRINWLEMLLVCEEEATFQHGTNTRRECRRVWQKTLWRHERFEILRSVPFE
                     TEAEIEIPHGMMHSFRSPHNEVRWRLYVRGSAQDWPNFERVFPLVVLPGPERERRPDS
                     RPGREGLSSETPSDEESPHQVNPGQASETYS"
BASE COUNT          495 a          563 c          518 g          427 t
ORIGIN      
        1 agtccggcca ggttcgtccg aacagaaact gtctacagat taggtgcagg tgactaccag
       61 aaaagttccc taatttgccc ttcgcctatc tataggattt ttggaatgcc gtagaagtca
      121 agggcgcgcc tcgccaaagc gggggacaga cttcagcaat cccgcgaaaa cttttacgtt
      181 acaaaaaacc acatcccttg aatctcgaga aaaaaacatg tgcttcaatt tatttacttc
      241 ctgtttgacg gataggaaat ccaggtccca ggctcgataa tcgttaccgg tcttccggga
      301 ccgcatgcaa gacttaggcg aagatcgaaa acagcttgcc ctgccacaag aaatcggcaa
      361 gcttcccttc aagagtcgtc ttgctccgac gatgacttag aagcctgtaa agcaccaagg
      421 gagtcggcct ccgatgtccg tcggacttcg tataaatcgt aaagatcaaa aagcatttct
      481 cgcaagtttc cttgatgacg cgcgaacgag gctcgaacat cgtcccgatt aataaggggt
      541 tgtccgcggg ctaggagata ttggacaatt tgccacgcca agtcctcgtt tggttcgggc
      601 gcaaagagaa ggggaatctc aatatcggtg tgggccgtaa caatcagtcc gagatgcttc
      661 tttcggcaga ggcggcgtaa ctgatagcga gaccaggcac ttaattgctc ataaccgtca
      721 acagtcacga cggtatcctg aagtgcttcc aatttccgcc atgttgcctg ccccagtgtt
      781 ctttggccat catggagctc gagcgccaga cacggttggc cattgttttc catccaatgt
      841 gtgaaggctg taataaaggc tgacttgccg gcaccatggg ggccgatcac cgcccccaga
      901 cgttggttag cctgaaagat ttcgtgaact gactcccatg aacatcccgg tggaaagata
      961 tagggaataa cccccggccg gacgaagcgg gccgaaaagg gattatccac ctgtcccagt
     1021 ggtgctttca tcgtagtctc acccgggccg gggctacgcg gccaagccgg aaggggacct
     1081 cttccacaag ttcacggccc cgtgcgcgaa agactcgcac gcgcacacac cagcgaattc
     1141 gaatgatcaa accgtcgtaa cttaacggtg tctgaggaag aaccgtttga aagcggagag
     1201 gttcacgcag gtcaagcggt aaatctccct cttgcgacca gcggcgaaaa taccgcacgt
     1261 gaaggtcctg atctcccttg ccatccgtgt accagagaat cgacagctcc accgctttga
     1321 tatcctgaag gggcaaaggc tctatccgaa cctgaccggc cagtacttcg ccgggttcgt
     1381 aaacagtctt ctttccatca agatggatca gtatccgtgg ctcggtcatg aatatgtttc
     1441 agaggcctga cctggattga cctggtgtgg ggattcttcg tcacttgggg tttccgaaga
     1501 cagaccctca cggcctggcc gggaatccgg tctccgctcg cgctctggcc cggggaggac
     1561 caccaacgga aagacccgct caaagttcgg ccaatcctgg gcactgccgc ggacatacaa
     1621 acgccagcgg acttcgttat gtggcgaacg aaaggaatgc atcatcccat ggggaatctc
     1681 gatttccgcc tccgtctcga aagggacact ccgcaggatt tcaaaccgct cgtgccgcca
     1741 gagggtcttt tgccagacgc ggcggcactc tcgtcgtgtg ttggtcccat gctggaacgt
     1801 tgcctcctcc tcacatacga gcaacatttc cagccagttg atcctcagcc aacccgtctg
     1861 cttcaaataa acccggtaac gatgtccaca gagaagtgga aaatcggaaa tctcgacaat
     1921 cgtttccccc acggcgctgg tggccatcca ctgccggaag aaggcaccga gcaaggtaag
     1981 accaatcacc aaacacggca ata
//
LOCUS       DAOPTR010000788         2002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_44279_length_2002_cov_2.061633, whole genome shotgun sequence.
ACCESSION   DAOPTR010000788 DAOPTR010000000
VERSION     DAOPTR010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44279_length_2002_cov_2.061633"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            404..1411
                     /locus_tag="PKI05_13205"
     CDS             404..1411
                     /locus_tag="PKI05_13205"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOA53205.1"
                     /translation="MRKITIFNHKGGVGKTTLVVNLADALADMGHRVLVVDADPQCNL
                     SAFHLPEDQLDHFLGESETEEAKGFNTLWAGLRPVVLGRGDVVSVDLFEVRENVLLLV
                     GDVLLSQYEEELPAAWVDSFARKPRGYDVMCAVSRLTEILCEAYHIDYVLVDIGPNVG
                     PLNRAVLLDSDYFATPVAADLFSLRALGAVGHTLGRWIQDWQTVRSLAPSSERDRLLK
                     GTPTYLGYINSAFKVREGDKKAKPHEYWERQMAPRISERLVGTLEGIDSGLVCGAPYL
                     LGEVKHFQSLPSEAQRYGIALSKLKGKVNPGHNAQIALAKDTFALLATKMLERIADHE
                     S"
     gene            1401..>2002
                     /locus_tag="PKI05_13210"
     CDS             1401..>2002
                     /locus_tag="PKI05_13210"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT domain-containing protein"
                     /protein_id="HOA53206.1"
                     /translation="MNPNELALSCVEKFRANHHRLQLFGQSVAAFFEDHPELRSERST
                     IHSVKWRMKDEDHLRAKVLRKIANGKKITPDNLFQSVTDLVGVRVLHLHQAQFPSIHG
                     EILKRVAEGDWVLEEDPKAFTWDPESKSFFEELKLATEIRSTFYTSIHYLVRPNPESP
                     LCCEIQVRTLFEEIWGEIDHLLNYPVPSESIACKEQLRTLA"
BASE COUNT          489 a          479 c          539 g          495 t
ORIGIN      
        1 aggaaagggc aattgcggat gaaaccgcga ctgccctcga acatgaggcg accggatgtg
       61 gggtcgcctt gtgttggtgg gtggatgcaa ggacgagagg gttgggctgc tgagagaggt
      121 gtttttgaag agcggcaagc ggctaggagc ggtcggccgt catccaatcc agatagcggc
      181 cattcgggca aggcggcatg ttctctgctt atgtggcgca agggttccat caccccacta
      241 tgccgtcagc ttcggcttgc cttatattgc tattttgcct tgcgcttagt ctgcgttttt
      301 ttaactgtca catgcagaaa atgttgtccg gcaaaaccgt cgccagacaa aacagttagc
      361 ttgcttgtca ggggtaaccg atccagtcat tcaaaagaac cagatgagaa aaatcacgat
      421 cttcaatcat aaagggggtg tcggaaaaac cacgctcgtg gtgaacctcg ccgatgcttt
      481 agcggacatg ggccatcgag ttctagttgt cgatgctgat cctcagtgca acctttcggc
      541 atttcatttg cctgaggatc aactcgatca cttccttggt gagtccgaaa cggaggaagc
      601 caaagggttc aatactttgt gggcgggcct tcgtccggta gtcctcggtc gaggagacgt
      661 cgtttctgtc gacctattcg aggtacgcga gaacgtttta cttcttgttg gcgacgtgct
      721 cctttcgcaa tatgaagagg agcttccggc agcttgggtc gattcgtttg cccgaaaacc
      781 ccgaggctac gacgtcatgt gcgcggtttc gcgcctgacg gagattctgt gcgaggcgta
      841 tcatattgac tatgtcctag tcgacattgg gccaaatgtc ggcccattaa atagggcggt
      901 tttgctcgac tcggattatt ttgcaacgcc tgttgcggca gacttgttct cattgcgcgc
      961 cctcggggct gttggccaca ctcttggacg ttggatacaa gattggcaaa ccgtgcggtc
     1021 gctcgcgcca agttcagagc gcgatagact cttgaaggga acgcctacct atctcggcta
     1081 tatcaattcg gctttcaaag ttcgtgaggg agataaaaaa gccaaacctc atgagtattg
     1141 ggagagacag atggccccac gtatctcaga gaggctagta ggaacgctcg aaggcatcga
     1201 ttcgggactt gtatgtggcg ctccctacct tctcggagaa gtgaagcatt tccaaagtct
     1261 cccatctgaa gcacagcgat atgggatagc gctttcaaag cttaagggta aggtcaatcc
     1321 aggccacaac gcccaaattg cacttgctaa ggacacgttc gcgttgctag ccacgaagat
     1381 gcttgaaagg attgcggatc atgaatccta atgagttagc tctttcgtgt gtagagaaat
     1441 tccgtgcaaa tcaccaccgc ctgcaactct ttggccagag cgttgctgca tttttcgaag
     1501 accaccccga actgagatcg gagcggagta ccattcactc agttaaatgg aggatgaagg
     1561 atgaggatca tcttcgtgcc aaggttttga ggaagatcgc caatggaaaa aagattacac
     1621 cagataatct gttccagtcc gtgactgacc tcgttggagt gcgtgtactg caccttcatc
     1681 aggcacagtt tcctagcatt catggcgaga tactcaaacg agtagccgaa ggggactggg
     1741 tgcttgaaga ggatccaaag gcatttacat gggatccaga atcaaagagt ttttttgaag
     1801 aacttaagct tgcgaccgaa atacgatcaa ccttttacac gtctattcac tatctcgttc
     1861 ggccaaaccc agaatcccca ctttgctgtg aaattcaggt tcgaacacta ttcgaagaga
     1921 tttggggtga aatcgaccac ttgcttaact acccagttcc atcggaaagc atcgcgtgca
     1981 aagaacaact gcgtacgctg gc
//
LOCUS       DAOPTR010000789         2000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_44327_length_2000_cov_3.782005, whole genome shotgun sequence.
ACCESSION   DAOPTR010000789 DAOPTR010000000
VERSION     DAOPTR010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44327_length_2000_cov_3.782005"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /locus_tag="PKI05_13215"
     CDS             <1..1208
                     /locus_tag="PKI05_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53207.1"
                     /translation="FIRQQKLALPPDQKTTHGFLYLSYGMDEMPAGKPEERRRRFIMP
                     PGAKWRVRLMARDGFYRPVGASASAKPVRIPAAVILEQAKAALWLLCHYGGVGSKGRN
                     GFGSLVLLDDGDTQNKRGELERAAAEFRHICGVNEQIEPHCQIPALRTALPEVLVSTP
                     WKNHWFVLDQLGYSLQSFAQKYKRKWIKEALGLPRRIGISDDDGSQSKHYQRDLDRKT
                     KQPVVWLGQKHPYRGNRKPKDMRHAAPFHFHIARSADGTYQIRVIAFPCAVLPDWKTS
                     NDVLQKLLAHLREDLQKRCDENSRDSTLVSPAIVSRALPRIPEKRPAGTPVQVKILAE
                     RPKGGYEVQEEGKPQGTLTVGTPPTERPEVGDIVSVEVHNDDPRRPQYRWPQNRPPMQ
                     SDKYPSSRR"
     gene            complement(1256..>2000)
                     /locus_tag="PKI05_13220"
     CDS             complement(1256..>2000)
                     /locus_tag="PKI05_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53208.1"
                     /translation="GTLSEGAPSGMADGQRAITIRPPFPVPEYGRPGDRMTLCATFLG
                     ERAAAAADVINRVLLESDRGFELGRDRVGFRLSAYLTEKDAPGALQCIEPEHLPASPE
                     DCRGYVPAVRLDLITPLFLKEEPVEGGKARAVLKPTLGQLVRGCLRTVGRAFAAFGKG
                     LDGRVDFAGLKKCAEAVPTQAAFWQPFRQRHHSTRQRQRYDLVGVTGGAVFGRVPLCL
                     LPWLVWGGRLGIGEHRVTGAGCWEVTLLA"
BASE COUNT          441 a          649 c          554 g          356 t
ORIGIN      
        1 agttcattcg gcagcaaaaa cttgccctgc cgcctgacca aaaaacgacc cacggtttcc
       61 tttacctctc ctacgggatg gatgagatgc cagcgggtaa gccggaagag cgcagacgcc
      121 ggtttattat gccccctggg gcgaagtggc gggtgcgcct catggctcgt gacggcttct
      181 atcggcccgt tggcgcgagt gcctccgcca aacctgtgcg gatccccgcg gccgtgattc
      241 tcgagcaggc taaggcggcg ctttggcttt tatgtcacta cggtggtgtg ggttctaaag
      301 gccgcaatgg atttggttcc ttggtattac ttgacgacgg agacacgcag aacaaacgag
      361 gcgaactcga gcgggccgcg gcggagtttc ggcacatatg tggggttaac gagcaaatag
      421 agccacattg ccaaatccct gctcttagga cagcgctacc cgaggtgttg gtttctaccc
      481 cgtggaaaaa ccactggttt gtactcgacc agctaggcta tagccttcag tcctttgccc
      541 aaaaatacaa gcggaagtgg atcaaggagg ctttgggcct tccgcggagg attggcattt
      601 cggatgacga cggttcgcaa agtaaacatt atcagcgcga cttggatcgg aaaacaaagc
      661 aaccagtcgt ctggctaggc cagaagcacc cataccgggg caaccgaaaa ccaaaggaca
      721 tgcgtcacgc cgccccgttc catttccaca ttgcccgcag cgccgacgga acctaccaaa
      781 tccgcgtcat cgcattccct tgtgcggtcc ttccggattg gaaaaccagc aacgatgtgc
      841 tccaaaaact ccttgctcac cttcgcgagg acctgcagaa acgatgtgat gagaactccc
      901 gggattcaac actggtgtct cctgctattg tgtctcgcgc gctcccgcgt attcctgaga
      961 agcggcctgc gggaacgccg gtgcaggtga agatcctggc tgaacgcccc aagggtggtt
     1021 acgaggtcca ggaggaggga aaaccccagg gcacgttgac cgtgggcacc ccgcctaccg
     1081 aacgccctga agtcggcgac atcgtttctg tcgaggtcca caatgacgat ccgcgccgac
     1141 cgcaataccg ttggccgcaa aatcgacctc ccatgcagtc cgacaagtat ccttccagcc
     1201 gtcgctagcc gccctgagag ggcaatcgtc aaggccgatt gtcaatcgcg agtgcctacg
     1261 ccaagagcgt tacttcccag cagccggccc cggttacgcg atgttcgccg atccccaggc
     1321 ggccgcccca aacaagccac ggcagcaggc acaacggcac gcgaccaaag accgccccgc
     1381 cggtgacccc cacaagatca taacgctgcc tctggcgagt gctgtgatgg cgttggcgga
     1441 aaggctgcca aaaggccgcc tgggtgggaa cggcctctgc gcacttcttc agccccgcaa
     1501 aatccacgcg accatctagc cccttgccga aggccgcaaa agcccgcccc accgtccgca
     1561 agcacccccg caccaattgt cctaaagtgg gtttcagcac cgcccgggcc ttgccgcctt
     1621 caaccggctc ttctttcaga aaaagcggag tgataaggtc caagcgaact gccggcacat
     1681 agccacgaca gtcctccggc gacgcaggca agtgctcggg ctcgatgcat tgcaaggcgc
     1741 ccggggcatc cttttcggtc agatatgccg aaagccgaaa gccaacccgg tcccttccaa
     1801 gctcaaaccc ccgatctgac tccaacaaga cacggttgat cacgtcggcg gctgccgccg
     1861 cccgctcgcc caaaaatgtc gcacacaacg tcatgcggtc gccaggccga ccgtactccg
     1921 gcacaggaaa aggcggacga atcgtgatcg cccgctgccc atcggccatg cccgagggcg
     1981 ccccctccga cagtgttccc
//
LOCUS       DAOPTR010000790         1998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_44389_length_1998_cov_3.483788, whole genome shotgun sequence.
ACCESSION   DAOPTR010000790 DAOPTR010000000
VERSION     DAOPTR010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44389_length_1998_cov_3.483788"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1345
                     /gene="gcvT"
                     /locus_tag="PKI05_13225"
     CDS             113..1345
                     /gene="gcvT"
                     /locus_tag="PKI05_13225"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOA53209.1"
                     /translation="MTDLKHTPLAGWHKENKAKMTPFAGWEMPLHYQSGILQEHLTVR
                     RYGGLFDISHMGRFRIAGPDTVRFLQYVLTSDVSKLGPWRAQYTILAKEDGSAIDDAY
                     LFRFGDEYWLVVNAANSAVDWQHLSEVAQGFSVQLEDLTAEVAMFAVQGPESETLLRQ
                     LLQDGALPDEGRNTLSSGTLAGVPVKLSRTGYTGEPIGFEVFVPAKAALALWESLVHA
                     GASLGLAPIGLGARDTLRLEAGLTLFGHELGTTPQGQPIPILAAPSAIVAVSLAEEKG
                     DFLGRKSLAAQVKCYRQWREGHAQKNPILPQIVRPVAILDAGVARQGDNVFWKGTPVG
                     QVTSGTMVPYWVFEGDDSHPGDQVMRRAIALALINIDVPKEAEIDVVVRGRSLKACIV
                     PRHGWSNRPPYFRPIVVR"
     gene            1356..>1998
                     /locus_tag="PKI05_13230"
     CDS             1356..>1998
                     /locus_tag="PKI05_13230"
                     /inference="COORDINATES: protein motif:HMM:NF012677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53210.1"
                     /translation="MSKKQSGSVSPPTSVREQVSTLLERSLENHLWRQQRCINLIPSE
                     MTPSPLVRLLQVTDPVGRYAEHNYLTQLGREVYYYQGTHFIEWVEQQLIDEFGRYLGT
                     DQIEPRLISGQTANMAVFSAVVAFRNRDNKLGEIERIRGVLNHHLNLGGHLSAQAMGP
                     LRDYVARDPTTGRFMIKHFPVCADNPYRVDVEATAALLDDWAPELVIFGKSMFL"
BASE COUNT          435 a          532 c          595 g          436 t
ORIGIN      
        1 ccattatgat gaagaaatac tttagcggtg gaaatgacga caccgcggta agacggaagg
       61 gttttcggaa cggacatcgc ggaacagttt cgaggaaaaa ggaagtatca aggtgaccga
      121 tttgaaacac acccccttgg cgggctggca caaagaaaac aaagccaaaa tgacgccgtt
      181 tgccggctgg gaaatgccgt tgcactacca gtcaggtatc cttcaggaac atttaacggt
      241 gcgacgctat ggcggccttt ttgacatcag ccacatgggg cggtttcgca tcgcagggcc
      301 tgacacggtg cgttttcttc agtacgttct taccagtgat gtcagcaagc tgggaccgtg
      361 gcgggcacaa tataccatac tggcaaaaga agatggttcg gccatcgacg atgcctacct
      421 ctttcgcttt ggcgacgagt attggctcgt cgtcaatgcg gcgaacagtg cggtggattg
      481 gcagcatctc agcgaggtgg cccaggggtt ttccgtgcag ttggaagacc tgaccgcgga
      541 agtggcgatg ttcgctgtgc aaggaccgga gagtgaaacg ctcctgcgtc aattacttca
      601 agacggagcc ttgcccgacg agggccgtaa caccctttca tcgggaacgt tggctggtgt
      661 ccccgtcaaa ttatctcgaa ctggctacac cggggaaccg attgggtttg aagtatttgt
      721 ccccgcaaag gcagccctcg cgctatggga atcgctcgtc catgccgggg cgtcgctagg
      781 gcttgctccc atcggtcttg gagcccgcga tacgctgcga ctggaagcgg ggttgacgct
      841 gtttggacac gaactgggga ctactccaca aggtcagccc atccccattt tggcggcacc
      901 gtctgcgatc gttgccgtct cgttggctga ggaaaagggt gactttctag gccggaagag
      961 cctagcagcc caggtgaaat gctatcgcca gtggcgcgag ggccatgctc aaaaaaaccc
     1021 catcttgccc cagatcgttc ggcccgtcgc catccttgat gccggcgttg ctcgacaggg
     1081 agataacgtt ttctggaaag gaacgcctgt gggccaggtc acaagtggta caatggttcc
     1141 ttattgggtt ttcgagggag acgactctca ccccggtgat caggtcatgc gacgggcgat
     1201 tgccctggcg ctgattaaca ttgatgtccc caaagaggcg gagatcgatg tcgtcgtgcg
     1261 tgggcggtcg ctcaaggcgt gtatcgtgcc gcgtcacggc tggagtaatc gtcccccata
     1321 ctttcgaccg attgttgtgc gataaggcat ggtctatgtc taaaaagcag agcggttcgg
     1381 tttcaccccc cacgagtgtc cgggaacaag tcagcacgct tctggagcgc tctttggaaa
     1441 accatctgtg gcgtcagcag cggtgcatca atctgatccc ttcggagatg acgccttcgc
     1501 cgctggtgcg attgctccag gtcaccgatc ccgtgggccg atacgcggag cacaattatc
     1561 taacccagct cggccgcgag gtctattact atcagggaac ccatttcatc gaatgggttg
     1621 aacagcaact gatcgatgag tttggacgtt acctgggcac ggaccagatc gagccgcgcc
     1681 tcatcagcgg ccaaacggcg aacatggccg tatttagcgc cgttgtggcc tttcgcaatc
     1741 gggataacaa actgggggaa attgaacgaa ttcgcggggt actcaatcac catctcaact
     1801 tgggcggaca ccttagcgcc caggctatgg gaccattgcg agactacgtg gcgagagatc
     1861 cgaccacggg acgcttcatg atcaaacatt tccctgtttg tgcggacaac ccataccgcg
     1921 ttgacgtgga ggccacggcg gctctgctgg acgattgggc gccggaattg gtcatcttcg
     1981 gcaagagcat gtttctgt
//
LOCUS       DAOPTR010000791         1998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_44391_length_1998_cov_3.311374, whole genome shotgun sequence.
ACCESSION   DAOPTR010000791 DAOPTR010000000
VERSION     DAOPTR010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44391_length_1998_cov_3.311374"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="PKI05_13235"
     CDS             complement(<1..635)
                     /locus_tag="PKI05_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53211.1"
                     /translation="MAKSQSGGIDKILANIWEKIVLGVAILITLGLIAMSATSGPNID
                     ITPEQLTQLASRAEQNYANTKPDVIRENESFSAPRYSEVAKRIREAVSSTGYQIVAPF
                     DPPMFEQRKKRGQPPVLPVSDLRAAAGHGAVAGLGDTGSGSVGVGSGYPGYPGSSPTM
                     PTSPYPTATSTYPQMYGESETGYAGYSGYGGYGGPTGPSHGRRWVVVTGII"
     gene            complement(733..>1998)
                     /locus_tag="PKI05_13240"
     CDS             complement(733..>1998)
                     /locus_tag="PKI05_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53212.1"
                     /translation="GEEAYGETMGYPGAGGYPGTGATGTGAAAIEMVGVVEWNPQNIA
                     AIKQRFAWRQVPTSDEIWNAQEDLWVYEALLRIIAKTNEGATGPHNAAVKRINAIQIG
                     PDAAAYFASASTSAMASATGMMASPGMGSPGMETGMPSEPSETGGEYGSGYGGYGVPG
                     MRSTFGRYVDASGNPLRPGDPQPFAEFKMMPVRLDLVVDQRKIDQLLVNCANSDMPVR
                     ITRVLIRPGQASRGGAGYGGFGGEAGMMSTGGYGYGSETGYETGASPGTGYGAGGTTQ
                     ASSSHDVPIVIEGVIYLFNPPDQEQLGKQGAPELVSGQSLAPIEAPPVETGDASQGGA
                     PSQPAGAAAPQAQPGQPEPQAQPAGAQPAPAGPMAPAVPPQASQPGTPTAPPSAPQGL
                     PPAVQPAPGTGPTPVPPGTQPGQPAAANP"
BASE COUNT          386 a          617 c          535 g          460 t
ORIGIN      
        1 tcaatgattc ccgtaaccac aacccaccga cgaccgtggc tcggccccgt aggtccccca
       61 tatcctccgt atcccgagta cccggcatag cccgtctctg actctccgta catctggggg
      121 taggtggaag tcgctgtggg ataaggagaa gttggcatgg tggggctact gccgggatat
      181 cctggatagc cactcccaac accgaccgac ccagatccgg tatcccctag acccgccaca
      241 gcgccgtgac cggctgccgc cctcaaatcg gaaacgggca agacaggcgg ctggccgcgt
      301 ttctttctct gctcgaacat cggtggatcg aacggggcga cgatttgata tccggtggaa
      361 gagacagctt ccctaattcg ttttgccact tccgaatacc tgggcgctga aaaactttca
      421 ttctctctga tcacgtcggg tttggtgttg gcgtagttct gctccgcacg actggcaagc
      481 tgggtcaatt gctcaggcgt aatgtcaatg ttagggccac tcgtggccga catcgcgatt
      541 aatcccaacg tgataagaat cgcaacgcca agtacgatct tttcccagat gttggccaga
      601 atcttatcaa ttccaccgga ttgtgacttg gccattttca atccctcgca acaaaggccc
      661 acacacttgg gtcaggactt ttcctctcgg ttagccaatc ggacttttgc tcccgctatc
      721 atcatacctt cactatgggt ttgctgctgc aggttgccct ggctgtgtcc caggcggaac
      781 gggtgttggg cccgtaccag gtgccggttg cactgctggc ggcaaaccct gcggcgcact
      841 gggcggtgct gttggggttc ccggctgact tgcctggggc ggtactgccg gagccatcgg
      901 ccccgctggc gccggttgag ccccggccgg ttgagcctgt ggttcaggct gaccaggttg
      961 agcctgtggg gcagctgctc cagcgggctg tgaaggggcg ccaccttgtg atgcgtcccc
     1021 cgtctcaact ggcggagctt cgataggcgc caatgattgt ccggagacca actcaggagc
     1081 cccttgtttt ccaagttgtt cctgatccgg aggattgaat aggtaaataa caccctcgat
     1141 cacgatgggg acatcgtgcg atgatgaagc ctgggtggtt cctccggctc catatccagt
     1201 tcccggggat gcaccagtct cataacctgt ctcgcttccg tatccataac cgccggttga
     1261 catcatcccc gcctcgcccc caaatcctcc atatccagct ccacctcgac tcgcttggcc
     1321 aggcctgatc aagacccgtg tgatacgcac cggcatatcg gagttcgcac agttgacgag
     1381 tagctggtca atcttacgct gatcgacgac caagtccaaa cggaccggca tcatcttgaa
     1441 ttcggcaaat ggttggggat cgccggggcg aagcggatta cccgatgcat caacataacg
     1501 gccaaacgtg gaacgcatac ccggtacacc ataaccgccg tagccgcttc cgtattcacc
     1561 accggtttcg ctcggctcac tcggcatacc agtctccatg cccggggaac ccataccggg
     1621 agaggccatc atcccagtgg cacttgccat cgcagatgtg cttgccgaag cgaaatacgc
     1681 cgctgcatcg ggcccgatct gaatggcatt gatccgttta acggctgcgt tgtgtggccc
     1741 tgtcgcgcct tcatttgttt tggcaataat tcgcaaaagt gcctcgtaaa cccacaaatc
     1801 ctcttgtgcg ttccatattt cgtcagacgt ggggacctgc cgccatgcga atcgctgttt
     1861 gatggctgcg atattctgag gattccattc cacgacaccc accatttcga tagcagccgc
     1921 tccggtacct gttgccccgg taccgggata gcctcctgcc ccgggatatc ccattgtttc
     1981 tccgtaggct tcttcacc
//
LOCUS       DAOPTR010000792         1995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_44474_length_1995_cov_3.677320, whole genome shotgun sequence.
ACCESSION   DAOPTR010000792 DAOPTR010000000
VERSION     DAOPTR010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44474_length_1995_cov_3.677320"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1985)
                     /gene="uvrA"
                     /locus_tag="PKI05_13245"
     CDS             complement(<1..1985)
                     /gene="uvrA"
                     /locus_tag="PKI05_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOA53213.1"
                     /translation="MGYEEGAILETAWEELPEQIQEILLFGENARNVPWAVKEKLVWN
                     SRITGGVAGILEVLPETLVQASAREKLQEARRVSVCPSCQGSRLRPEARFIRLQTSHP
                     RFEDNPSRSLPEVCALPVADTRLFFTDLVLDQIQQKIAEEPLKEIRNRLDFLLNVGLD
                     YLTLDRSAPSLSGGEMQRIRLASQIGSGLVGVTYVLDEPSIGLHPRDNDRLLATLAKL
                     RDQGNTVVVVEHDEDTIRAADYIVDFGPGPGSRGGQIVAAGSFEDIVKSPDSLTGQYL
                     TGKRCIPVPTKRRQPTDTKLIIRGARHNNLKNIDVEIPLGLFVCVTGVSGSGKSSLVS
                     DILVEALHRDLNGGVGQPGAFDRIEGLEHLDKMIAIDQSPIGRTPRSNPATYTKLFDD
                     IRKLFASLPEAKVRGYKPGRFSFNVKGGRCEACEGHGATKLEMDFLADIWVTCPVCNG
                     QRFNRLTLQVLYKGKSIADVLEMEVREALELFENIPPIRHKLETLHAVGLDYMKLGQP
                     SPTLSGGEAQRVKLARELVKRSTGRTIYILDEPTTGLHFADIEMLLKVLHSFVEAGNT
                     VVVVEHNLEVIKTADWIIDLGPEGGEAGGWVVAAGTPEDLAKVEASHTGRFLRMALAG
                     ETLQGNGEEKKRKAFFESLKTQRQQSKPITSVSVWGAR"
BASE COUNT          429 a          608 c          512 g          446 t
ORIGIN      
        1 cgggcacccc aaacactaac cgacgtgata ggtttacttt gttgcctttg ggttttgagg
       61 ctttcgaaga aggccttacg cttcttttcc tcgccgttac cctgcagggt ttctccggct
      121 aaggccatcc gaagaaaccg acctgtgtgc gaggcctcta ccttagccag gtcttccggg
      181 gtcccagcag cgacaaccca acccccggcc tcccctcctt cgggtcccag gtcgataatc
      241 cagtcggccg ttttgattac ttccaggtta tgttccacaa ccaccacggt gttgccggct
      301 tcgacaaaac tgtgtaacac cttgagaagc atctcgatgt cggcaaagtg caaacccgtg
      361 gtaggctcat cgaggatgta gatggtccgt ccggtgctac gtttgacgag ttctcgtgcc
      421 aatttgactc gctgggcctc gccgccggac agtgttggcg agggctgccc caatttcatg
      481 taatccagcc ccacggcgtg gagggtttcc aatttgtgcc gaatgggagg gatattttca
      541 aacagttcta gggcctcgcg gacctccatt tccaaaacgt cggcgatcga cttccccttg
      601 taaagcacct gtaatgtcag ccgattaaag cgttgtccgt tgcaaacggg gcacgtaacc
      661 caaatgtcgg ccaaaaagtc catttccagc ttcgttgccc cgtgaccttc gcatgcttcg
      721 caacgtccgc ctttcacgtt gaagctaaag cgccccggct tatacccgcg gaccttcgcc
      781 tccggcaaac tggcaaacag tttgcggatg tcatcgaaca atttggtgta agtggcggga
      841 ttggaacgcg gtgtccgccc gatcggtgat tgatcaatag cgatcatctt gtccagatgt
      901 tccagcccct cgatccggtc aaaggcgccg ggctgcccca ctccaccatt gaggtcccga
      961 tgaagggcct ccaccaggat gtcgctaacc aaagaactct tgcccgaccc ggagacaccc
     1021 gtgacacata caaacaagcc caacgggatt tccacgtcga tgttcttcag gttattgtgc
     1081 cgtgccccgc gaatgatcaa ctttgtgtcc gtgggctggc gacgcttggt gggcacagga
     1141 atacagcgtt tccccgtcag gtactggccg gtcaggctgt cgggtgattt aacgatatcc
     1201 tcgaaactgc cagcagccac gatctgtccg ccgcgactgc cgggcccagg accaaaatcc
     1261 acgatatagt ctgccgcccg aattgtatcc tcatcgtgtt ccaccacgac aactgtgttg
     1321 ccctggtcac gaagtttggc cagtgttgcc aagagccggt cgttgtcgcg gggatgcaac
     1381 ccgatggatg gttcgtccaa aacgtaggtg acgcccacca ggccagaacc gatttgactg
     1441 gccaagcgaa tccgctgcat ctccccgccc gaaaggctcg gcgcggagcg atccaacgtc
     1501 aaataatcca gccccacgtt caaaaggaag tcgagacgat tgcggatttc tttgaggggc
     1561 tcttcagcga tcttctgctg gatctgatcc aaaaccaaat cggtaaaaaa caggcgtgtg
     1621 tcagcaaccg gcaaagcgca aacctcgggc agtgatcgtg aagggttatc ttcaaatcgc
     1681 ggatgcgacg tctgcaagcg aatgaatcgt gcctccggtc gaagccgaga tccctggcat
     1741 gagggacata ccgacacacg tcgcgcctct tgcaactttt ccctggcaga ggcttgcacc
     1801 agcgtttcgg gcaacacctc aagaatgccg gcaaccccgc ccgttatgcg agagttccac
     1861 accaatttct ccttaaccgc ccatgggacg ttccgggcat tctcgccgaa aaggagaatc
     1921 tcttgaatct gttccggcaa ttcctcccag gccgtttcca agattgcccc ttcttcgtaa
     1981 cccaactcct cctca
//
LOCUS       DAOPTR010000793         1993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_44548_length_1993_cov_2.805470, whole genome shotgun sequence.
ACCESSION   DAOPTR010000793 DAOPTR010000000
VERSION     DAOPTR010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44548_length_1993_cov_2.805470"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PKI05_13250"
     CDS             <1..420
                     /locus_tag="PKI05_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53214.1"
                     /translation="AETSTSLAAVLEAVVPQVQGRVICVASLPRNPRQELGKWLELID
                     AHCGLGVITLANPGREDPAILSPLAYESMKSPRRIVIPDRTEAIGWALTEAREGDCVL
                     VIGNGHRRWRPLKENDLEVDDFQFIRQWLEQEFPHVT"
     gene            complement(805..1701)
                     /locus_tag="PKI05_13255"
     CDS             complement(805..1701)
                     /locus_tag="PKI05_13255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOA53215.1"
                     /translation="MSDYQEDSDIQPSAPGAPTSATLEEALAKCQIALLPEQVRLLDQ
                     YFQLRREWNQKMNLTRHDTYDQFVARDVVDSLSFMRCLQPDEVILDVGTGSGVPGVVL
                     SILRPDLCVVLSETVGKRARAVADIVSRLGLKIPMLIGRAEKYLEAGGWEFHSLVIRA
                     VAPLLNLLRWFRPYWDCFDRMLILKGPRWVTERGQARHYGLMHGLALRVVDRYTIPGT
                     GAESVLLQICPEERLIPPRECRFRKLSCEEMITGVVESPQPGPFAKKGTVPPAAKHQK
                     KPGPKKPSVSKGPGKNPGRRPK"
     gene            complement(1777..>1993)
                     /locus_tag="PKI05_13260"
     CDS             complement(1777..>1993)
                     /locus_tag="PKI05_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOA53216.1"
                     /translation="VAVRRAGAIHTFDSLNHYFSIAQMITVGSSYWNVGRGLQPGEVS
                     SDEEGIRTMQTLGENMAWVIKKLYGTT"
BASE COUNT          438 a          536 c          591 g          428 t
ORIGIN      
        1 gcggaaacgt ccacgtcttt agccgcggtg ttagaagcgg ttgtgcccca ggttcaggga
       61 cgtgttattt gtgtggcgtc gctgccgcgc aatccacggc aagaacttgg aaagtggttg
      121 gagttgatcg acgcgcattg cggactgggc gttatcaccc tcgccaatcc cggacgcgaa
      181 gacccagcaa ttctatcacc gttggcctac gaatctatga aatcgccccg gcggattgtc
      241 ataccggaca gaacggaggc cattggttgg gccttgacag aggctcgcga gggggattgt
      301 gtcttggtca tcggtaatgg acaccgccgc tggcgcccct tgaaagagaa cgatttagaa
      361 gtggacgact tccagttcat ccgccagtgg ttggagcagg aattccccca tgtaacgtga
      421 atagggcttg ttaaaaggct ttctcagacg gtgctgggag gccacagaga aaagtcgagc
      481 aaggaggttt ggaagataca cagcacgaca tccattgcaa gccagtgaca accccatcga
      541 ttgcgttagg tttgcgggag cctgatgcag ccctccctcc tggggtgaag catgaagctt
      601 gtcggatgat gcggtttctg ggtcggccgc tccgacgagc gcttctcgat ggggttcggt
      661 gacctgaggg ccggagcggt gcgggtcaca ccgctccggt cacctcaggg agaaggactc
      721 atcggcagtc gatggcgatt cgtcaccttc gcggaatgac tcttcggtcc tcggtcttgg
      781 tagctcaaat gatctccgga cgagttattt tggtcttctg cccggatttt tgcccgggcc
      841 tttgctgacg gaaggcttct tgggacctgg cttcttctga tgtttggcag ccggtgggac
      901 ggtgcctttt ttggcaaacg gacccggttg aggtgattcc acaactcctg tgatcatttc
      961 ttcgcaagac agcttgcgga aacggcattc acgcggcggg atgagccgtt cctcgggaca
     1021 gatctggaga aggacgcttt cggctccggt tcccgggatc gtgtatcggt caaccacccg
     1081 cagggccaac ccgtgcatca ggccatagtg ccgtgcttgg ccgcgctcgg tgacccaacg
     1141 ggggcctttg aggatcaaca tccgatcaaa acaatcccag taaggacgaa accagcgaag
     1201 tagattcaga agtggggcca ccgcacgaat aacgagggaa tggaactccc atccgcctgc
     1261 ttccaggtat ttctcggccc gaccaatgag catcgggatt ttcagtccta aacgagacac
     1321 aatatccgcg acggcacgag cacgtttgcc gacggtctcg ctaaggacaa cacacaagtc
     1381 tgggcgaaga atcgacaaaa ccacgccggg gacaccactg cccgtcccaa cgtctaggat
     1441 cacttcatca ggctggaggc atcgcatgaa cgataaggaa tccaccacgt cccgggcaac
     1501 gaactgatcg taggtatcat ggcgggtaag gttcatcttc tgattccatt cccgccgaag
     1561 ctgaaaatat tgatcgagaa gccgcacctg ctccgggagg agcgcgattt ggcacttggc
     1621 caaggcctct tccagtgtgg ccgaagtagg tgcccctggg gcggagggtt gaatgtcaga
     1681 atcttcttgg tagtcggaca tgcgaaatca aaatcggtca cgcaaacggg gtaagtaatg
     1741 cttggactcg gacgacagag gaagacgggg aatctgtcac gtcgtaccgt acagcttttt
     1801 gatcacccaa gccatgtttt ctccgagggt ttgcatggtg cggatacctt cctcgtcact
     1861 actgacctcg cccggttgca gaccgcggcc cacattccaa tagctggaac ctacagtgat
     1921 catttgggcg atggagaaat aatgattgag cgagtcgaac gtatgaatcg cgcccgcccg
     1981 tctcacggcc acg
//
LOCUS       DAOPTR010000794         1992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_44566_length_1992_cov_3.962313, whole genome shotgun sequence.
ACCESSION   DAOPTR010000794 DAOPTR010000000
VERSION     DAOPTR010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44566_length_1992_cov_3.962313"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1992
                     /locus_tag="PKI05_13265"
     CDS             <1..>1992
                     /locus_tag="PKI05_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53217.1"
                     /translation="GDVSLLFSNGDGGIICRFYPNYHQSWAAYQAVVDSLNDNRTWQP
                     IALLATDQGRYRQSGMGSVLVFWENSDLILACGGTRLLTVPFRDPPKEIRLAGTTLLR
                     ELRWLDGVPPPPLDSERLFPDAFDHPTSPLFLASRMVVNGSIPASQPWERWPQSKEVT
                     VHWDESGEIGLSAEDVRERTYLGFPIVVNGVCDIVFRVREATPGTGVFLADKDRRPIG
                     GLGFYRHTQSGRLLFGFHTAQDDNWERGADPNQLLAITDGSQWFRITVSAGVLRYWVS
                     CDGKVWSPAGPFNDHADRRPAFCGIFLTKGPTRKIVLRDLYVYEGLVPSDEKLARLTE
                     EVPDSIATASDWERWCELVVRNCPDTSTLQEWRMACAFRSIAENPPAHLGQRILEQLW
                     DDVLSLDLPMHVKLNCSAVFARGVSQVDWGIYERLTPRLRRVVWQLAADDPSHAFTVI
                     TEHLLRIPYWAQWRLPVFFTDLYRHELFAALAKNDTEHLQQLGLRVLRASVPGNDAVS
                     DELRYLAVSTAARTQATDVMPNETPSQARQSLVIVPNREAYNFAQEIAAALADNDPRQ
                     VTQLILLAGREEMRSLYPDPLDPGRYCSFPLFVHNLLEQYPAVRQLVLEQCQEIGKVQ
                     LRQAQSSGREDLAEEILCRFPETPIAAEAALWLGDRYLVL"
BASE COUNT          407 a          543 c          586 g          456 t
ORIGIN      
        1 gggtgatgtc tcgctacttt tctcaaacgg cgacggtggc atcatatgcc ggttttaccc
       61 gaactaccat caaagctggg cggcttatca agcggtggtt gattccctta atgacaatcg
      121 cacgtggcag ccaatcgccc tcctggcaac ggatcaaggc cggtatcggc aaagcggcat
      181 gggaagcgtt ctggttttct gggaaaacag cgatcttatt ttggcctgcg gtgggacacg
      241 gctgttgacg gtaccatttc gagatccccc caaggaaatc cgcttagcgg gaacgacgct
      301 tctgcgggaa ctgcgttggc ttgatggagt gcctccaccg ccgctcgaca gtgaaaggct
      361 ctttccggat gcgttcgacc atcctacgtc cccactattc ctcgcctcaa gaatggtggt
      421 caatggatcg atcccggcga gccagccgtg ggaacgctgg ccccagtcga aggaggtgac
      481 ggttcattgg gacgaaagcg gcgagatcgg cctatcggca gaggatgtga gggagaggac
      541 gtatttaggc ttcccgatcg tcgtcaacgg cgtctgcgac attgtcttcc gggtgcgcga
      601 agccacacca ggaacgggcg tcttcctagc ggataaggat cgtcggccta tcggcggact
      661 gggtttttat cggcacacac agtcgggaag gctactcttt gggttccata cggcacaaga
      721 tgacaattgg gaacgtggtg cagaccccaa ccaactgctg gccatcaccg atggctccca
      781 atggtttcgg atcactgtct cggcgggtgt cctgcgatat tgggttagtt gcgatggcaa
      841 ggtgtggagt ccggcaggcc ccttcaacga tcacgccgat cgtcgcccgg ccttttgcgg
      901 aattttccta accaagggac ccacgcgaaa aatcgtgctg cgagaccttt acgtctatga
      961 agggcttgta ccttcagatg agaagctcgc gagattgacg gaggaggtcc cagacagcat
     1021 tgcgactgcc tctgactggg agcgttggtg tgagctcgtc gttcgtaact gtccggacac
     1081 gtcaacgctc caggaatggc gcatggcttg cgcgtttcgg tcgatcgccg agaatccacc
     1141 ggcacactta gggcagcgga ttttggaaca gctctgggac gacgtcctta gtctcgattt
     1201 gccaatgcac gttaagttaa attgctcggc ggtttttgca cgaggggttt cccaggtgga
     1261 ttggggtatc tacgagcggc tcacgcctcg tttgcgtcgt gtggtgtggc aactggccgc
     1321 cgacgatccg agtcacgcct tcacggtgat tacagaacat ctactgcgta ttccctattg
     1381 ggctcaatgg cgattgccgg tgttttttac ggacctttat cgtcacgagt tgttcgcagc
     1441 cctggcgaag aacgacactg aacatctcca gcaacttggt ctgcgggtcc taagggccag
     1501 tgttccggga aatgacgcag tttccgatga attgcggtac ctggcggttt cgaccgcagc
     1561 acggacccaa gccaccgacg tcatgccgaa tgagacgccc agccaagccc gacaatccct
     1621 cgtgatcgtg cccaatcgtg aggcatacaa tttcgcccaa gagatcgcag cggcgctggc
     1681 cgataacgat cctcgccagg taacacagtt gattcttttg gctgggcgtg aagagatgcg
     1741 aagcctttac ccggatccct tagaccccgg aaggtattgt tctttcccgc tgtttgttca
     1801 caatctgttg gaacagtatc ctgccgtgcg acaactggtc cttgagcagt gtcaggaaat
     1861 cggcaaagtc caattacgac aggcccaatc ctccggtcga gaggatctgg ccgaggaaat
     1921 cctctgtcgc tttccggaga caccaatcgc cgcggaagcg gcactgtggt tgggggatcg
     1981 ttatctcgtt ct
//
LOCUS       DAOPTR010000795         1991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_44618_length_1991_cov_2.570248, whole genome shotgun sequence.
ACCESSION   DAOPTR010000795 DAOPTR010000000
VERSION     DAOPTR010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44618_length_1991_cov_2.570248"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PKI05_13270"
     CDS             <1..547
                     /locus_tag="PKI05_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA53218.1"
                     /translation="EKKNLQLTVDIQPNLPPMFQDQNRVAQILNNLLSNAIKFTPEGG
                     AIRVIVRRDNNGDLILRVADTGVGIAEEDRQIIFEKFRQGRTAMPDGDPLKREYPGSG
                     LGLSIVKELCRLLGGEVGVESELGRGSVFTVRLPWRLEIAEEGDQPPYFRPINAEEMR
                     FDRDRVSKRLRPTPASEKGPR"
     gene            620..1108
                     /gene="rnhA"
                     /locus_tag="PKI05_13275"
     CDS             620..1108
                     /gene="rnhA"
                     /locus_tag="PKI05_13275"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOA53219.1"
                     /translation="MKARRQQENHRPQVQLFTDGACSGNPGPGGWAFILYHPASGKKL
                     IQSGAERDTTNNRMELMAVIKGLQALRKPSRVQLFTDSIYVGRGLTEWLAAWKARGWK
                     RGKNEPVKNEDLWRQLSELLETHQVEFHHVHGHSGHPENETCDRLAVEAYQKLLKHPQ
                     KP"
     gene            complement(1158..>1991)
                     /locus_tag="PKI05_13280"
     CDS             complement(1158..>1991)
                     /locus_tag="PKI05_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOA53220.1"
                     /translation="YLVMTATPIPRTIAMTLFGDLDLSVLNESPPGRKPVRTYLATSD
                     RRSQWWEFFRKKLREGRQGYVVVPLVEEGDEEVRSVVATYEELSRGELAGFRLGMIHG
                     RMSGEEKDKVMADFREGRIDVLISTSVVEVGIDVPNATLMTIENAERFGLAQLHQLRG
                     RVSRGKHPGFCCLFSDSTSPESRERLKAFCSTTNGFELAEIDFALRGPGELLGTRQHG
                     LAPFRIADILRDSDLVLEARRNAWDLVREDPGLARPQHARLRQMVLRRYGKSLSLADV
                     G"
BASE COUNT          479 a          544 c          547 g          421 t
ORIGIN      
        1 agaaaagaag aacttgcaat tgacagtcga tatccaaccc aatttaccgc cgatgttcca
       61 agatcagaat cgcgtcgccc agattctcaa caatctcctt tccaacgcca tcaaattcac
      121 tccagaaggc ggtgccattc gcgtgattgt gcgtcgcgat aataacggtg acctgatttt
      181 gcgtgttgcc gatacaggtg tcgggatcgc cgaagaggat cgtcaaatta tctttgaaaa
      241 atttcgccag gggcggacgg ccatgccgga cggcgatcct ctcaaacggg aataccctgg
      301 ttcagggttg ggcctctcca tcgtaaagga actttgtcgg ctattaggcg gtgaggtagg
      361 tgtggaaagt gagctggggc gggggagcgt ctttacggtt cggttgcctt ggcggcttga
      421 gatcgctgaa gagggagatc aaccaccgta ttttcgtccc ataaatgccg aagagatgcg
      481 attcgatcgt gatcgtgtca gtaagcgact gcgacccacg ccggccagtg agaaaggtcc
      541 gcggtgacgg ccagtggggg tcatcctgga aaaacagctc gttcattccc ggttatttcg
      601 aggggaattg tgaggtgcca tgaaggctcg ccgacaacag gaaaaccatc gaccccaagt
      661 gcagctattc accgatgggg cctgtagtgg aaacccaggt cctggtggct gggcgtttat
      721 tctttatcat ccggcttcag gcaaaaagct gattcaatca ggagcagagc gtgatacgac
      781 caacaatcgc atggaactga tggctgttat caaggggctc caagcgctgc gtaagccaag
      841 tcgtgttcag ctcttcacag acagcatcta cgtgggacgc ggtctcacag aatggctcgc
      901 cgcatggaag gcacgcggtt ggaagcgggg aaagaacgag cctgtcaaga acgaagactt
      961 gtggcgccaa ctgagtgagc ttttggaaac tcaccaagtg gagtttcatc acgtgcacgg
     1021 ccatagcggc catcctgaaa acgagacctg tgaccgtctg gccgtggaag cgtaccagaa
     1081 gctcctcaag catccccaaa agccttgaga agtctcggcc atttcggttc caaacaccgg
     1141 ctgacagttt ccggaaacta accgacatcg gccagtgaga gcgattttcc gtaacgacgc
     1201 aataccattt gacgcaatcg agcatgctgg ggacgagcca gacccgggtc ctcacgaacc
     1261 aggtcccatg cattgcgacg tgcttccaaa accagatccg agtcgcggag gatgtcggca
     1321 atgcggaacg gggccaggcc atgttgacgt gtcccaagta attcaccggg gccacgaagg
     1381 gcgaagtcga tctctgccaa ttcaaaacca ttcgtcgtgc tgcaaaaggc cttcagtcgt
     1441 tcacgggact ctggcgaagt cgagtccgag aacagacagc agaaaccagg atgcttgccc
     1501 cgcgagacgc gcccccggag ttggtgaagc tgggctagac caaatcgctc cgcattctca
     1561 atggtcatca atgtggcatt gggaacgtcg atgccaactt ccacaacgga ggtggaaatg
     1621 agaacatcga ttcgtccctc ccgaaaatcc gccatgactt tgtccttttc ctctccactc
     1681 attcgtccgt ggatcatccc caatcggaat ccggcgagtt ccccacggct taactcctcg
     1741 taagtggcta cgacactgcg aacctcttca tcaccttctt ccacaagtgg cacaacgaca
     1801 tagccctgcc gaccttcgcg aagtttcttg cggaagaatt cccaccactg gcttcgtcga
     1861 tcacttgtcg ccagataggt ccgaacgggc tttctgcccg gcggggattc gttcagcacg
     1921 cttaaatcca agtccccaaa caaggtcatc gctattgtcc gtgggatcgg tgtggccgtc
     1981 atgaccaggt a
//
LOCUS       DAOPTR010000796         1986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_44779_length_1986_cov_3.948213, whole genome shotgun sequence.
ACCESSION   DAOPTR010000796 DAOPTR010000000
VERSION     DAOPTR010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44779_length_1986_cov_3.948213"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..144
                     /locus_tag="PKI05_13285"
     CDS             <1..144
                     /locus_tag="PKI05_13285"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOA53221.1"
                     /translation="DTPPRVVIDEAVELAKLFGSQQSGPFVNGILDRLYRDIMAQRGT
                     SVE"
     gene            337..1251
                     /gene="ftsY"
                     /locus_tag="PKI05_13290"
     CDS             337..1251
                     /gene="ftsY"
                     /locus_tag="PKI05_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOA53222.1"
                     /translation="MGLLDRIKQGLKKTAQLLRTDVRDLFKTQGRLVDQAFLDELFEI
                     LIRTDMGVQAAQQIVDQIGDKYRNRVIEWNQAIEEIKTILKQLLKQPESPIKFAENSP
                     TVIMVCGVNGCGKTTTIAKLANMFRSEGKQVILGAADTFRAAAVEQLSIWAQRLGVEI
                     VTGVQGSDPAAVAHRAATRALETGADICIVDTAGRLQTHQNLMRELSKIHRVLGKQIP
                     SAPHEVLLVLDATTGQNGISQAKHFRDAVNCTGLVLAKLDGTAKGGVVVAIRQQMGLP
                     VKFVGVGEKAEDLLPFDADTFVEAMFAD"
     gene            1584..>1986
                     /locus_tag="PKI05_13295"
     CDS             1584..>1986
                     /locus_tag="PKI05_13295"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA53223.1"
                     /translation="MSTSRLSRRSVLKLGAAIGLPVLVSSRALGLEGQAPASERVKIG
                     IIGLGGRARAIVNESREIKEMQIVGVSDCFKPAIDRFIKDVGQDQNWVGYEDFRAMIE
                     KEKPDGVMVETTTHARAWVTIHAMQMGMDVYI"
BASE COUNT          470 a          512 c          554 g          450 t
ORIGIN      
        1 gacacgcccc cgcgggttgt tattgacgaa gctgtcgagc ttgcaaaact ttttggcagt
       61 cagcagtcag gtccattcgt taatggtatc ctcgaccgac tctatcgaga catcatggcg
      121 caacggggta cttcggttga atgatgggct cgtttttgga cgcatcttgt gatccacttg
      181 gcaaggcagc cgttatacat gaaccaatca tccgagtttt cgaacaagat tgcccaactt
      241 ggtcctggcc gactgcaacg ataaacctct ggctcagctt tgcgagaatt tgagaagggc
      301 taacgaacat cactgctggt gcgatgggtg aaaactatgg ggttacttga ccgtatcaaa
      361 cagggactga agaagacggc acaactcctt cggacagacg tccgcgacct attcaagaca
      421 caagggcgtc tcgtcgatca ggcgtttctc gatgaattgt tcgaaatctt gatccgaacc
      481 gatatgggag ttcaggccgc ccaacaaatt gtggaccaga tcggggacaa atatcgaaat
      541 cgggtcatcg aatggaacca ggcgatcgag gagatcaaaa ccatactcaa gcaactcctc
      601 aaacaaccgg aatccccgat caagtttgcc gaaaacagtc cgaccgtgat tatggtttgt
      661 ggcgtcaacg gatgcggcaa gacaaccacg attgccaagc tggcaaatat gttccgttct
      721 gaagggaagc aggtgatctt aggtgctgct gacacatttc gggcagcggc tgtggaacaa
      781 ctgagcatct gggcgcaacg cctgggagtg gaaattgtga ctggggttca gggaagcgat
      841 ccggctgccg tggcacaccg tgcggcgacc cgtgctctcg aaacgggtgc cgacatctgc
      901 attgtcgata cagcaggccg gctccaaaca catcaaaacc tcatgcgcga gctctccaag
      961 attcatcgcg tactgggtaa gcagattccc tccgctcccc atgaggtcct gcttgtcctc
     1021 gatgcaacaa caggacaaaa cggcatcagc caagcgaaac actttcgcga cgcggtgaac
     1081 tgcacaggat tggtgcttgc aaagctggac ggtacggcca agggcggcgt ggtggtggcc
     1141 atccgtcagc aaatgggact cccagtcaag ttcgtgggcg tcggggaaaa ggctgaggac
     1201 ctattgccct tcgatgccga tacgttcgtt gaggccatgt tcgccgacta agggcgatct
     1261 ctcatcatta ccgtttcctc atacacaacc tttctcgttc aataggcgag tttgatgcgg
     1321 ctacgatggc atgtggactt ctaacacatc gggcaggcac aggggccttg tctctcttga
     1381 tggttagagc actcacgtcc tgccttggcc cctggtcgcc cggtacccag ttggaatcgc
     1441 atcgggaaat ggttatccta ttcatcagcc aagcggtcag gacccaagtt atgtttcctc
     1501 gattttgatt gacaaggggt gcgccgcagt tcacaatcac cgaaaagttt acgttgtctt
     1561 ttgctgatga aaggaatcca accatgtcca cgtctagatt gtcacgccgt tctgtcctaa
     1621 aactgggtgc cgcgattggg ctgccggtcc tcgtttccag tcgtgcgttg gggttggagg
     1681 gtcaggcccc tgcttcggag cgcgtcaaga ttggcatcat tggactcggc gggcgggccc
     1741 gcgcaatcgt taacgaatcc cgcgaaatca aagagatgca gattgttggc gtctcggatt
     1801 gttttaaacc tgccatcgat cgtttcatca aggacgtcgg ccaggaccag aattgggttg
     1861 ggtacgaaga tttccgggcg atgatcgaaa aagaaaagcc cgacggagtc atggtcgaaa
     1921 cgacaacgca cgcccgtgcc tgggttacca ttcatgccat gcagatgggg atggatgttt
     1981 acatcg
//
LOCUS       DAOPTR010000797         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_44817_length_1985_cov_3.847150, whole genome shotgun sequence.
ACCESSION   DAOPTR010000797 DAOPTR010000000
VERSION     DAOPTR010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44817_length_1985_cov_3.847150"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PKI05_13300"
     CDS             <1..724
                     /locus_tag="PKI05_13300"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HOA53224.1"
                     /translation="LHGLAFWLAHLEEQRHRKQPELIRAIRKFRRKAAILEMGQWIKR
                     QSREMVAAGSSQGGLPVSITKQHRKLLSERLADLLRTAQSLEDPQDIEGHHAMRIAAK
                     KLRYSMEALAPCLGVIGEVTIEALRSLQSHLGEIHDYDVWVERIRQVLDMISGGKFDG
                     PRWLNPDRFMPGLVFLQNKYQELREVRFRQLQNLWHDEKIQGLWQRLQASGFQAISED
                     NAKYADGARNREDHPDVKTQHP"
     gene            844..>1985
                     /locus_tag="PKI05_13305"
     CDS             844..>1985
                     /locus_tag="PKI05_13305"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53225.1"
                     /translation="MTTNEGEDTVHPVAVIDIGTNAIRMVIAQVFPSGEFEVLERLQR
                     PIRLGKDTFSRGRIGAQSMRSVVAILRDYQAVMNSYGVQQVRAVATSSLRDAANADNV
                     LDRIYLACRLNVEIIGIAEEGRLTVAAVREVVEDLPEVNQGKTLIVDVGGGSTILTML
                     QEGEIVNSIGLRFGSVRILEMLSLQSEPPERVAEVLQQHIRTQIANVERSLTLQDCDT
                     FVAVGGDVRFAARVVGHPTRSEKLFVVEKDQFRSLLRQCSRHTPNQLAHKYNLPFIDA
                     ETLLPALMTYWELLCRTSAERVIVSQVSMRDGLLLELAREATGRKDNALQEGIVRAAE
                     AVGQRYRVDLDHARLVAGIAIKLFDFLVADHGLGPRQRLLLQVAALL"
BASE COUNT          453 a          488 c          592 g          452 t
ORIGIN      
        1 tctccacggt ctcgcctttt ggctcgcaca cctcgaggaa cagcggcatc gaaagcagcc
       61 tgagttgatt cgagcgattc gcaaattccg caggaaggca gcgatcctag aaatgggcca
      121 atggatcaaa aggcagtccc gagagatggt agccgcgggt tcctcgcagg ggggattgcc
      181 cgtgtcgatc acgaaacagc atcgcaagct tctgtcggag cgattggcgg accttttgcg
      241 aacggcccag tcgctcgagg atccgcagga tattgaaggt caccacgcca tgcggatcgc
      301 ggcaaagaaa ctacggtact cgatggaggc actggccccg tgcctgggtg tgattgggga
      361 agtgaccatt gaagcgcttc gcagtcttca gtcacatctg ggcgaaatcc acgattacga
      421 cgtgtgggtt gagcgaatcc gtcaagtgct agatatgatt tctggcggga aatttgatgg
      481 cccaaggtgg cttaatcccg atcgttttat gccgggcctg gtttttttgc aaaacaagta
      541 ccaggaactt cgcgaggtcc ggttccgtca gctccagaat ttgtggcatg atgaaaagat
      601 tcagggattg tggcaacggc ttcaggcctc tggttttcaa gccatctcgg aggacaatgc
      661 aaagtatgcg gatggtgcga gaaaccgtga ggaccacccg gatgtgaaga cccaacatcc
      721 ctgaagaagt gaatgtcact ttttgagcag gttttctcaa tggcttgtcg cgaattagaa
      781 aagccaaccg aaaaaacgtt tgtatgaaaa atcgttgtta tgtcggacct tgttgatagt
      841 cacatgacga caaacgaggg cgaagacacc gtccacccgg tggcggtgat cgacatcggg
      901 acgaatgcaa ttcgcatggt gatcgcccag gtttttcctt ccggggagtt tgaggtcctg
      961 gaacggttgc agcgacccat caggctgggg aaggatacct tttcacgtgg gagaattggt
     1021 gcccaaagta tgagatcggt ggtggctatc ctccgcgact atcaagcggt tatgaatagc
     1081 tacggggtgc agcaagtaag ggcggtagct accagttcgc tacgtgacgc cgcaaatgcc
     1141 gacaatgtgc tggatcgcat ttatctggct tgccggttga atgtagagat catcggaatc
     1201 gcggaagagg ggcgactgac cgtggccgct gttcgcgaag tggtagaaga cctcccagaa
     1261 gtcaaccaag ggaaaaccct cattgtggat gtcggcggcg ggagcacgat cctgacgatg
     1321 ctccaggagg gtgagattgt caattccatc ggcttaagat tcggctcggt acgaattctc
     1381 gaaatgttgt ctttacaatc ggagcctccc gaacgtgttg ccgaggtttt gcagcaacat
     1441 attcgcacgc aaatcgccaa tgtcgaacgc agtctgaccc ttcaagattg cgacaccttt
     1501 gtggcagtag gtggagatgt tcgctttgca gcgagagtag tgggtcaccc cacgcgttcg
     1561 gagaagctct tcgtagtgga aaaggaccag ttccgttcgc tacttcggca gtgtagccgt
     1621 catactccca atcagcttgc ccacaaatac aatctgccgt ttatcgacgc cgagacattg
     1681 ctacccgctc ttatgacata ttgggaactg ctgtgtcgca cctcagcgga aagagtcatt
     1741 gtgtcgcagg tctcgatgcg agatgggtta cttctggaac tggcccgaga ggcgacaggc
     1801 cggaaggaca acgcccttca agaggggatt gttcgggcgg cggaggcggt cggtcagcgc
     1861 tatcgggtgg acctcgatca tgcccgtctg gttgcgggca tcgcgatcaa actgttcgat
     1921 ttcctggttg ccgaccatgg cctgggacct cggcaacgtc tgcttcttca agtggctgcc
     1981 ttgct
//
LOCUS       DAOPTR010000798         1984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_44846_length_1984_cov_3.739762, whole genome shotgun sequence.
ACCESSION   DAOPTR010000798 DAOPTR010000000
VERSION     DAOPTR010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44846_length_1984_cov_3.739762"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /locus_tag="PKI05_13310"
     CDS             <1..1106
                     /locus_tag="PKI05_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53226.1"
                     /translation="EKAGVPSDHVRIAATHTHSMPAFCFMRQWGAIPEDFMRTVEERT
                     VEAACRAIDDLAPAELLVGRSTAVGGSHNRTSKNFKTEDEFNAAATDEDRWLDRTIHV
                     LLFPRKGKRTLCWYHFSAHAVCYADELAGPDWPGEVAARLREEEGLDPGFLQGHCGDV
                     NPGDGSDWRGEIKQTVSAIYPALKKAIATVVPVNCRPLRSVRAEFRLPYDMDLFQSWL
                     KSYRESPDQCKSGQWVDAGFAEDWYRGNVNRDLSQKDLPITLSALRLGDVAMVFHPSE
                     LYTCYGLTIRRDSGFPHTLVVGYTDGFIGYLPDPKAYQAGEYAALTVPKILDYPPFQP
                     EAGRIMTAAAVKILYQLRDYPKKFPSPSERGSG"
     gene            1260..>1984
                     /locus_tag="PKI05_13315"
     CDS             1260..>1984
                     /locus_tag="PKI05_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53227.1"
                     /translation="MVAQVPAHTMTAKGRLFWIKPVTWFAVLAILIDVMIVASALAET
                     QGQGILLSQDGVAQCTIVVPQESSPKELELASELAHWLKEMTGATFDVSRGLRDQAII
                     LGTWDEWRTLLSADMEPPKGPEGYRIRSDRKNLWIIARSELGLQHAIYDFLECLGCRW
                     YFPDPVWTVIPRVPTLVIQLDRTAVPAFGYRRIWYGWGPRTDKLARDYQDWLKHNRQL
                     GSFAVSCGHSYDSHIPPSQFAEHP"
BASE COUNT          434 a          525 c          570 g          455 t
ORIGIN      
        1 ccgaaaaggc cggcgtgccg agtgaccacg ttcgaattgc ggcgacgcat acgcattcta
       61 tgcccgcctt ttgtttcatg aggcaatggg gcgccattcc ggaagacttc atgcggactg
      121 tggaggagcg gacggttgag gccgcttgtc gtgccatcga cgatctcgcc cccgcggaac
      181 ttctcgtggg gagaagcaca gccgtcggcg gcagccataa ccggacatcc aaaaacttta
      241 agacagaaga tgagtttaac gccgcggcga cggatgaaga ccgttggttg gatcgtacga
      301 tccacgtgtt gctctttccc cgaaaaggaa aacgaacgct ttgttggtat catttttcgg
      361 cacatgccgt gtgttatgct gatgaactgg caggtcctga ttggccgggt gaagttgcag
      421 ctcgattgcg cgaagaagaa gggcttgatc cgggttttct tcagggtcat tgcggcgatg
      481 tgaaccctgg tgatggttct gattggcgag gagaaatcaa gcaaaccgtt agcgccattt
      541 atccggcctt gaaaaaggcc atcgccaccg tggtgccagt caattgccgg cctcttcgtt
      601 cggtgcgggc agagtttcgt ctcccgtatg acatggacct ctttcagagt tggttgaaat
      661 cctatcgaga atcgccggac cagtgcaagt ctggccagtg ggtcgatgcg ggattcgccg
      721 aagattggta tcgggggaac gttaatcggg atttgtctca gaaggaccta ccgatcacgc
      781 tgagtgcgct tcgtctcgga gacgtggcaa tggtctttca tccgtcggag ctttacactt
      841 gctacgggct gacgatacgt cgcgattccg gcttccccca cacgcttgtg gtgggctaca
      901 cggatgggtt tatcggttac ctcccggacc caaaagccta ccaggcgggt gagtatgctg
      961 ccttgacggt ccccaagatt ctcgattatc caccgttcca accggaggcc ggccgcatca
     1021 tgaccgccgc agcggtcaag atcctttatc aactaagaga ctatcccaag aagttcccct
     1081 ctccctccga gagagggtcg gggtgagggc aagcgaacgt cggaatcgat tgagaaaacc
     1141 gccagtgatt caagcccccc tcacccccga cccctctccc gccaaatggg cgaggggaga
     1201 ttatggaata ggcattaagc agatgacttc taggaccaac cattcgtcgg agcataagta
     1261 tggttgctca agtgcctgct cataccatga cggctaaggg gcgtctattt tggataaaac
     1321 cggtcacgtg gttcgcggtg ctcgctattt tgatcgatgt gatgattgtc gcgagcgccc
     1381 tcgcggagac gcaggggcaa gggatcttgc taagccaaga tggtgttgct caatgtacaa
     1441 ttgtcgtgcc gcaggagtcg tcccccaagg agctcgaact cgcttctgag ctggcccact
     1501 ggctaaaaga aatgaccgga gcaaccttcg atgtttcacg aggattgcgc gaccaggcaa
     1561 ttatcttggg aacctgggac gagtggcgta ccttgttaag tgccgacatg gagccaccca
     1621 aagggccaga gggataccga atccgcagcg atcggaaaaa cctttggatc atcgctcgaa
     1681 gtgaactggg tcttcagcac gcgatttatg actttcttga atgtctggga tgtcggtggt
     1741 attttccgga ccccgtttgg acagttatcc cacgcgtgcc cacattggtg atccaacttg
     1801 atcgcacagc agtccctgct tttggttacc gccgaatttg gtacggctgg gggccaagaa
     1861 cagacaaact cgcccgcgac tatcaagatt ggttgaaaca taatcgccaa ctcgggtcct
     1921 ttgccgtgtc atgcggtcac tcgtacgata gccatattcc cccgagtcag tttgcggaac
     1981 atcc
//
LOCUS       DAOPTR010000799         1983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_44882_length_1983_cov_3.101141, whole genome shotgun sequence.
ACCESSION   DAOPTR010000799 DAOPTR010000000
VERSION     DAOPTR010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44882_length_1983_cov_3.101141"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1155..1706)
                     /locus_tag="PKI05_13320"
     CDS             complement(1155..1706)
                     /locus_tag="PKI05_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53228.1"
                     /translation="MSKTKRLHVGSFRFNPPPEPYPDLDLKMVLRCNPYLEYQYRAVG
                     FGNTFEEALENLFEKIRISGFVVDRRTRYRITNALSYGGWETSCRLPSAKPQEDELAT
                     AIGTLLVAMVEQPEDVPTPQVLYSISIAWNALEDEKQFTRPQGAKRLFGGFPIAKWLR
                     KATDWLAWPIRALLNISKLSWNT"
BASE COUNT          462 a          527 c          535 g          459 t
ORIGIN      
        1 tcaagcggac cggactaaac gtcatagatc aagtcaaaga cccctcgtag gggcaggccc
       61 ctgtgcctgc gatatgcgtt cccatgacct tgtcaacggc cgagtggcaa acggaagctt
      121 tccccagccg ctcgagtggc attaagttaa caacttatca agaaggtcaa aagggcttcc
      181 ccgcgcagta ggaaaaacga ttcggatttt ttattactga cgatttttcc tccgactaaa
      241 tcgctcgaaa tactcgtacg gaccccggtt tatttgtttg gacagatctt gtccaacttc
      301 gcccccagaa agggttgaca acagcgttcg gctcgccgga tcggccatga tccggccccc
      361 gttccctaag cccgccggca aaacgcatgg tcttcggcga ccgggcgaga ctcagcgttc
      421 gcggtgaccc aatcactaga cggcggggac gccgtccctc tcggggctat tcaatgattg
      481 ggcagttgtc gccacgtgtg ccaggggctg taagcaatga tttgcccgtc cgttagtgga
      541 gcaaaccggc cagatgcagc tagtagctat tgcccggcct gagtacctcc cccgcctggc
      601 cgccgaacgt ccctgcgaac caatctttgg gattcagctg cgcatccatc ctggtcgtaa
      661 acaccactgt ccatggcttg ttctccgcgg tggatagcgg gtttacaatc actccctgcc
      721 ctagagcgaa gcccgttgtt attttcaggt ccgttttggg agaacgcttc tgctgacgcg
      781 ctacgcttgt ggcgaggacc tatttaaagc ctatcccata atctcccctc gcccgttcag
      841 cgggagaggg gtcggggtga gggtggctcg aatcgctgcc ggttttctca atcaattccg
      901 acgttcgctt cccctcaccc caaccctctc ccggagggag agggggactt tttgggataa
      961 acgctaatga gaaagattag acaaaggaga gggccagggt ggtggattaa cccgggttgg
     1021 catccagcgg gatgatcaaa acggggttct ccgagctaca ttccgcctgg tgccggtcgt
     1081 tccaagcgga ccgtcagcca ggcgaaagtg ctctgagact aaactactcc tgacccttct
     1141 cctcagtaat gtgtctatgt attccatgaa agtttcgaaa tgtttaatag agcacgaatt
     1201 ggccacgcta gccagtcggt ggccttgcgt agccacttcg cgatagggaa tccaccgaac
     1261 aggcgtttag ctccttgggg tctggtaaac tgcttctcat cctccagggc attccaggcg
     1321 attgagatgg aataaagtac ctgcggtgtt gggacgtcct caggctgttc taccatcgct
     1381 acgagcagtg tgcctattgc agtggcgagt tcatcctcct gcggtttggc cgagggcaac
     1441 cgacaagagg tttcccaacc gccgtacgat aatgcgttag taatgcggta ccttgttctg
     1501 cgatcgacta cgaacccgga tatccgaatt ttttcgaaca gattttccag tgcctcctca
     1561 aacgtgttgc caaacccgac cgcgcggtac tgatactcca agtaagggtt acagcgcagg
     1621 accatcttga gatctaagtc tggatatggc tcgggaggag gattgaaccg aaacgagcca
     1681 acgtgaagcc gcttggtttt cgacatgaga atctccttgt ctgggtgttt gttgtcacaa
     1741 aagatcgacc agaagccggt gaaagatata ccggcgcagg atacaacgcc gagatcgcgg
     1801 gaatacaaga accagagtga ttaagcttga gataagggag tacagcctaa atcacatcga
     1861 taacatttac gagctaatag gagggctgag atatgaagag acagaatagg acgcgataga
     1921 cgcggtttac atatgaaagg atatatctct gtatatctct tttgatagcc tacatcagcc
     1981 ggg
//
LOCUS       DAOPTR010000800         1982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_44901_length_1982_cov_3.523093, whole genome shotgun sequence.
ACCESSION   DAOPTR010000800 DAOPTR010000000
VERSION     DAOPTR010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44901_length_1982_cov_3.523093"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PKI05_13325"
     CDS             complement(<1..673)
                     /locus_tag="PKI05_13325"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOA53229.1"
                     /translation="MMKMRISPKVEKGNYAMIHEMKIFSGRANVELAQKVADYVGLPL
                     GRISLGRFPDGEISVKIEEDVRGRDIFIVQPTCPPVNENLMELLIMIDSFKRASAARI
                     TAVIPYFGYARQDRKDEGRVPITAKLVANLITRAGADRVLAMDLHAPQLQGFFDIPVD
                     HLYAAPVLDAYFLSLGIPLEDIVVVSPDEGSIKRTLLHVPVFGGKLAIVDKRRSGNST
                     SAAHLI"
     gene            complement(741..>1982)
                     /locus_tag="PKI05_13330"
     CDS             complement(741..>1982)
                     /locus_tag="PKI05_13330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA53230.1"
                     /translation="LGNQMTMARRYLNERLARMVLLKSSPSLMKRAHISVLHLAGRLN
                     PGGIETWLLQVLQAAIETHFHMDVLVVTDQPGELDDAIRQAGARLFVTNRPLNPLGQL
                     HDLRAIHRQWGPYDIIHSHVQPGGIQLRLAQHCGISIRIAHSHTTELLAPGLRFHQKI
                     VARLTKSWVRRYATHGLACSERAAETLFGKNWRDDPRWQVLHYGIDLTPFRERGERPG
                     ELRHSLGLPEDAFVIGHVGRMSPEKNHKFLLTLAQALVTRMPETYLVLVGDGPVRRQV
                     EKWIEQKGLTERVRLLGSRRDVPALMMNLFDLFLFPSIYEGLPLVVIEAQAAGLPIVA
                     SSAVPEEAIVIPQLIRRLSLGEPLTAWVEAVTAARQSAHAISRDHCLSLMERSDFNIE
                     TCTRKLLQIYQEAVSSTRPLD"
BASE COUNT          450 a          542 c          551 g          439 t
ORIGIN      
        1 caatgaggtg agccgctgag gtactattcc cagaccgacg tttgtcaacg atcgccaact
       61 tccccccaaa aacggggacg tgcaaaaggg tgcgcttgat tgacccttcg tccgggctaa
      121 ccaccacgat atcctcgagg ggaattccca ggctcaaaaa ataggcgtct aacactggcg
      181 ccgcataaag gtggtcaacc ggaatgtcga agaatccctg caattggggt gcgtgcaaat
      241 ccatggccaa aacgcggtca gccccagcgc gggtaatgag attggccact agtttggccg
      301 tgatgggcac acggccttca tctttcctgt cttgcctagc atacccaaaa taaggaatca
      361 ccgctgtgat ccgtgccgca cttgcccgtt tgaagctatc gatcatgata agcagttcca
      421 taaggttctc gttaaccggg gggcaagtcg gctgcacgat gaagatatcc cgcccgcgga
      481 catcctcctc gatcttgacc gagatttccc cgtcagggaa acggccgagt gaaatccgcc
      541 caaggggcag gccgacgtag tctgccacct tttgagccag ctcaacattg gcccggccac
      601 taaaaatctt catttcgtgg atcatggcat aattaccctt ctcgaccttc ggagatattc
      661 tcattttcat catggggcgc ttttgcgccg gtgaccagcg ggaccgactt cgttggcctc
      721 caattgtcac gtgttctggc ttaatccaac ggccgagtgc tactcacggc ttcttgataa
      781 atttgaagca actttctcgt acacgtttcg atattgaaat cgctgcgctc catcaatgag
      841 aggcaatggt cccgtgagat cgcatgggca ctttgtctcg ctgccgtgac agcctccacc
      901 caggcggtga gtggttcacc gagcgaaagt cgccggataa gttgggggat cacgatagcc
      961 tcctcgggga ccgcggagga agcgacgata ggcagcccgg ctgcctgcgc ttcgatgacg
     1021 acgaggggaa gcccttcgta aatggaggga aatagaaaca agtcaaagag gttcatcatc
     1081 aaggctggga catcacgccg ggagcccaag aggcgaacac gctccgttag gcccttttgc
     1141 tcaatccatt tttccacctg gcgacgaacg ggaccgtcgc caacaaggac caggtaggtc
     1201 tctggcatcc gtgtcacaag ggcttgggcg agcgtcagca agaacttatg gttcttctcg
     1261 ggcgacatcc gtccaacatg gcctatgaca aaggcgtcct caggtaggcc aagagaatgt
     1321 cggagttcac cgggccgttc gcctctttcg cggaagggtg taagatcaat gccatagtgc
     1381 agaacttgcc aacgtgggtc atcgcgccaa ttctttccga aaagagtctc cgccgccctt
     1441 tcgctacagg ccagtccgtg tgtcgcgtat cgcctgaccc atgattttgt cagacgggcg
     1501 acaatctttt gatggaatcg taaccctggt gcgagcaatt cggttgtatg ggagtgggcg
     1561 atgcggatcg agataccaca gtgttgggca agccgaagtt gaatcccccc aggttggaca
     1621 tgggaatgaa tgatgtcata cgggccccat tgccggtgga tagctcgcag gtcatgcaac
     1681 tggccgagcg gatttaatgg ccgatttgtg acgaaaagtc gtgctcccgc ttgtcgaatc
     1741 gcgtcatcca gctcacctgg ctggtcggtt acaactagca catccatgtg aaaatgcgtc
     1801 tctatggcgg cctgcaagac ctggaggagc caggtttcga tcccgccggg attgaggcgg
     1861 cccgccaaat ggagcactga aatgtgtgca cgcttcatga gtgatgggga actcttcaag
     1921 aggaccattc tcgcaagtcg ctcattgaga taacgtcttg ccatcgtcat ttgattcccg
     1981 ag
//
LOCUS       DAOPTR010000801         1982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_44907_length_1982_cov_3.065387, whole genome shotgun sequence.
ACCESSION   DAOPTR010000801 DAOPTR010000000
VERSION     DAOPTR010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44907_length_1982_cov_3.065387"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PKI05_13335"
     CDS             complement(<1..503)
                     /locus_tag="PKI05_13335"
                     /inference="COORDINATES: protein motif:HMM:NF014458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOA53231.1"
                     /translation="MLDRRFILENTDAVKRNCEIRGVVADVDRFVELERQRKELQSQL
                     DAINRQANEVAKSIGRCTTAEDRESKKEEGRRLREQAAQIEKQLQQIAAESDSILKQI
                     PNMTHPAAPIGDETASREVGRGKTPIRQFDFVPKDHVQIAEELDLIDFEAGARVTGHG
                     FYFLKNEA"
     gene            complement(633..>1982)
                     /locus_tag="PKI05_13340"
     CDS             complement(633..>1982)
                     /locus_tag="PKI05_13340"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOA53232.1"
                     /translation="TTAEQFLSEFLAIPSVSADPQHRPDMKRAAEWVAQFFSQMGLAA
                     ELIQSPTGHPVVYAETPHCEGMPTVLIYGHYDVQPAEDPTLWQTPPFEPTIRGGAIYA
                     RGASDDKGQVLTHLLSVGAWYQVTKSWPLNFKFVIEGEEEVGSSVLEKFLDEQADRLA
                     CDCIVISDGNQFGPDQPAITYGLRGIAYFELRVKGPHRDLHSGSFGGTVTNPATALTR
                     MLGQFMTQDGRVAIPGFYDDVLPLSNEERRRLRELPFDEPAFWASLGVETAWGEPGYT
                     VLERRWARPTFEVCGLWGGYQGDGAKTVIPANAGAKISFRLVPHQDPDRIREALLSYL
                     AQICPPGVQYEVIDLHASPAVLTSLDSPYIKAAENAVKMAFSRSPVFTREGGSIPIVS
                     RFQQRLGADVLLLGWGQDDDNTHAPNEKFSLADFHRGTLASAYLWEFIRQIGKATDS"
BASE COUNT          428 a          546 c          534 g          474 t
ORIGIN      
        1 gcttcgtttt tgaggaagta gaatccatgt ccggtcacac gggccccggc ctcaaaatcg
       61 ataaggtcca attcctcagc aatttggacg tgatccttcg gcacaaaatc gaattgccga
      121 atgggcgtct ttccgcgtcc cacttcacgg gaagctgtct catccccgat gggtgcggcg
      181 ggatgggtca tattgggaat ctgcttaaga atggagtccg actccgccgc aatctgttga
      241 agctgttttt caatttgtgc tgcttgttcc cggagccgac gtccctcttc cttcttgctt
      301 tcccggtctt ctgccgttgt gcaacgtcca atggatttgg caacctcatt ggcttgccga
      361 ttgatggcat ccagttgact ctgtaattct ttgcgctgtc gctctagctc gacaaaacgg
      421 tcaacgtccg ctaccacccc acgaatctcg caatttcgtt tgacagcatc cgtgttttct
      481 agaataaagc gtctgtcgag catactttta ttctcctgat gtcacatccg gttccgtact
      541 tccttcacat cagtccgagc tggggaataa cggtttggtt gggactttgg ccacgattcc
      601 aagcgagcag ccgtacattt cgtgaggggc tttcagctat ccgttgcttt cccgatttgt
      661 cttatgaact cccaaagata ggcactcgcc agggtccccc gatgaaagtc agcaagcgaa
      721 aacttctcgt ttggggcgtg agtgttgtcg tcatcctgtc cccaaccgag taaaaggaca
      781 tcagccccca accgttgttg gaagcggctg acgataggaa tggaaccacc ttcgcgggtg
      841 aaaacagggg acctggagaa ggccattttg acagcatttt ccgcggcttt gatgtaaggg
      901 ctgtccaaag acgtcaagac cgccggtgaa gcgtgtaggt caatgacctc atattgaaca
      961 ccgggcggac aaatttgggc gaggtagctg agcagtgcct cgcgaatgcg atcggggtct
     1021 tggtgaggaa ccagtcggaa actgattttt gctccggcgt tcgccggaat gacggttttg
     1081 gcaccatccc cttgataacc accccacagg ccacagacct cgaaggtcgg tcgcgcccaa
     1141 cgcctttcca aaacggtgta gcctggttct ccccaggcgg tttccacacc caaggaggcc
     1201 cagaaagccg gctcgtcgaa gggtaattcg cgcaaacgcc gacgttcctc gttacttagg
     1261 ggaagaacgt cgtcatagaa accggggatg gccactcgcc cgtcctgcgt catgaattgt
     1321 cccaacattc ttgtcagagc tgttgccgga tttgtgaccg tgcccccgaa cgatccagaa
     1381 tggaggtccc gatgaggacc cttaactcgt agctcgaaat aggcgattcc acgcagcccg
     1441 taggtaatcg caggctggtc gggtccgaac tgattcccat cgcttatgac aatacagtca
     1501 caggcaaggc gatctgcttg ttcatctaag aacttctcaa ggacggagct gcccacctct
     1561 tcttcgccct ctatcacaaa tttgaaatta agcggccagc tcttcgtcac ttggtaccaa
     1621 gccccgaccg acagtaagtg ggtgagcacc tgccccttat cgtcacttgc accacgcgcg
     1681 taaatggcac caccacgaat agtgggctca aacggtggtg tctgccaaag ggttggatct
     1741 tcggcgggct ggacgtcata atgtccgtaa atgagcacgg tcggcatacc ctcacaatgt
     1801 ggggtttcgg catagacgac tgggtggcct gtgggagact ggattagctc cgcggcaagc
     1861 cccatttgag agaaaaactg ggccacccac tcggcggcgc gtttcatgtc agggcggtgc
     1921 tgaggatcgg cactgacact tgggatggcg aggaattccg agagaaattg ttctgccgtg
     1981 gt
//
LOCUS       DAOPTR010000802         1980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_44971_length_1980_cov_2.840000, whole genome shotgun sequence.
ACCESSION   DAOPTR010000802 DAOPTR010000000
VERSION     DAOPTR010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44971_length_1980_cov_2.840000"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..>1980
                     /locus_tag="PKI05_13345"
     CDS             193..>1980
                     /locus_tag="PKI05_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOA53233.1"
                     /translation="MWSYILRRLLLMIPTLFGITVVSFCIMQLAPGDPLLLQLGATGL
                     AGQTTQTREAYLIQKRDLKLDRPLLINLRYFRNYRDMVYWAGYYRAADELQIAAELAA
                     LSRDPQGIADGPRKLAFLRALKIPRFDQRLQDAREHRRLARAISIYVQVFCEDLGWHG
                     VGPAIAHLQDPATDRQIRIGLIHALNSMVVDPFVFTYSSRPTDKETPYVVAAWRLWWE
                     RAEATARPLSQEDRAELEARFHALCQMSQNELLEELDYLDPETVRFYAEKLLGDASLQ
                     ERFVASLALRRLVRQPLRLDVPLNALDTVLQEVIRNWSEHYQIYRSRYEPSLAARLAY
                     IFTDTQYAHMVWRLVTFQFGRSATKTREPVATKIWNAVVVSAPLMLMAELVIYFIAIP
                     AGIIAAVKRNTLIDRSISLALFLLYSIPSFVAAMLLLLFFCYGDYLKWFPMIGLHSPG
                     AENFSWPRYLLDYLWHAFLPVMCLALFSLAGLAMYARTSMLEVISQDYIRTARAKGLS
                     EFKVIFKHALRNALIPILTLFSNFLPALLGGSVLVEYIFGIHGMGRLSWESIEQKDYP
                     TLMALIYIDAIIVLVSILLTDILYVLVDPR"
BASE COUNT          386 a          584 c          525 g          485 t
ORIGIN      
        1 cgggatggtc caattttcgt gtacgggtcg gaacgatggg acggatgtga ccgtgataac
       61 gcgatcttgc cagggaaagg gcttctgacg taatcttccc acctcgcggg aaagcgaaat
      121 ggcgtataac cgagaagcat aagcgacaag caatggacct tagtcactga ctgaagacgg
      181 aacgaacgat ccgtgtggag ttacatcctt cgccgtctcc tgttgatgat accgacgctg
      241 ttcggaatca cggtggtgtc cttctgcatc atgcagttgg cgccgggtga tcccctcctc
      301 ttgcagcttg gtgctaccgg actggcaggc cagaccaccc agacccgcga agcctatctc
      361 atccagaagc gcgacctgaa gctggatcgc ccgcttctca tcaacctgcg ttatttccgt
      421 aattatcgtg acatggtgta ttgggcaggc tattaccgtg cggccgatga acttcaaatt
      481 gccgccgagt tggccgccct ttctcgggat ccgcagggaa tcgccgacgg ccctcgtaag
      541 ttggcttttt tgcgggcgct caaaattccc cgctttgacc agcgactaca agacgcacgc
      601 gagcatcggc gtttggcccg cgccattagt atttatgtgc aagtcttctg cgaagacctc
      661 ggctggcatg gcgtggggcc ggcaattgct cacctgcaag accccgcaac ggaccgccag
      721 attcgaatcg gcctgattca cgccctcaat tccatggtcg tcgatccctt tgtctttact
      781 tattccagcc gtcccaccga caaagaaacg ccctacgtgg tggcggcctg gcgcctgtgg
      841 tgggaacgtg cggaagcgac ggctcgccct ctctcccagg aagatcgggc agaactcgag
      901 gcacgtttcc acgccctgtg ccagatgtct cagaacgaac tcctcgagga gttggattac
      961 ctagatcctg agaccgtgcg gttttatgcg gaaaaacttc tcggcgacgc cagcctccaa
     1021 gaacgctttg ttgcaagcct ggccctacga cgactggtca gacagccctt gcggcttgat
     1081 gtgcccctca atgccctcga taccgtcctg caggaagtca tccgcaactg gtccgagcac
     1141 taccagatct atcggagccg ttacgaaccc agtcttgccg cacgtttggc ctacatcttc
     1201 accgacacac agtatgccca catggtgtgg cggttagtta cgttccaatt tggccgctcg
     1261 gccaccaaga cacgggaacc ggtggctacc aagatttgga acgccgtcgt tgtctccgcg
     1321 ccgttgatgc tgatggccga actggtgatc tactttatcg ccattcccgc cgggatcatt
     1381 gccgcagtca agcggaatac gctcatcgac cgctccatta gcctggcgct ctttttgttg
     1441 tattccatcc cctcatttgt ggcggccatg cttttgttgt tgttcttctg ttatggcgac
     1501 tacttgaagt ggttccccat gatcggtctc cattcgccgg gggcggagaa cttttcctgg
     1561 ccgcgctatt tgctggacta cctctggcac gcctttctgc cggttatgtg tttggcgctc
     1621 tttagtttgg caggattggc catgtatgcc cgcacaagca tgctcgaagt gatctcccag
     1681 gactatattc gcacggcacg cgccaagggc ctttcggagt tcaaagtcat cttcaagcac
     1741 gccttaagaa atgccctcat cccaatcctt acgctgtttt ccaacttcct tccggccctt
     1801 ttagggggaa gcgtcttggt ggaatacatt ttcggcattc acggcatggg ccgcttgagc
     1861 tgggaatcca tcgagcaaaa ggactatccc acgctgatgg ccttaatcta catcgacgcc
     1921 atcattgttt tggtgagcat tctcctgacg gacatcctgt acgtgttggt cgatccgcgg
//
LOCUS       DAOPTR010000803         1979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_44985_length_1979_cov_4.115385, whole genome shotgun sequence.
ACCESSION   DAOPTR010000803 DAOPTR010000000
VERSION     DAOPTR010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44985_length_1979_cov_4.115385"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1795)
                     /locus_tag="PKI05_13350"
     CDS             complement(56..1795)
                     /locus_tag="PKI05_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HOA53234.1"
                     /translation="MDHEAQPLKRSQVEKLKEESQYLRHPLAEEVNNDQPSFSESAAQ
                     ILKFHGSYQQDDRDARKLQRASGGGKVYFTMIRVKVPGGVLTAEQFLTQLDLCDQLGN
                     GTARITDRQDLQLHGVLKGNLRETIRRINEVQLTTLGACGDVVRNVMCCPAPLRHNRV
                     REQLQNLAQEITRHLLPRTPAYHEIWLTDPDTGEKEQVTEPSGEVAEVEPIYGKTYLP
                     RKFKIAFGLPDDNCVDLYTNDLGFLAVVENGEVVGFNVLVGGGLGVTPSNKNTFPALS
                     QPLVFVRPAEVVPVAEVVVKIYRDYGERGDRKRARLKYLIADRGLPWFREMTERYLGR
                     SLPDPLPLGVTEVDDHMGWHEQGDGRFFYGLNLENGRIVDRDAFRLKSALREICQRLH
                     PSIRFTPQQSVLFCDLEASARSEIEQILRRYGVPLSEEISNVRRWSMACVALPTCPLA
                     VTESERVLPKLIDDLEREVAKLGLDAERFTVRMTGCPNGCSRPYNADIGLVGRTQNKY
                     SIYLGGRLLGDRLAEPFAETVPLEKIVETLVPVLQLFAQERQDHETFGDFCHRVGVAT
                     LRARLIRGEGAAG"
BASE COUNT          453 a          576 c          514 g          436 t
ORIGIN      
        1 cgtaaaccga gccgtctggg ctaacgcgaa gaaatatcgc ggggatcgtt tgggattatc
       61 ctgcggctcc ttcgcccctt atcaagcgtg cgcgaagcgt ggccacaccc acccggtggc
      121 agaaatcgcc gaatgtctcg tggtcttgac gttcctgagc gaacaattgc aacacgggga
      181 caagggtctc gacgattttc tccaggggaa ctgtttcggc aaaaggctcc gcaaggcgat
      241 cgcctaatag gcgtccgcca agataaatgc tgtatttatt ctgtgtccgc cccaccagcc
      301 caatgtcagc gttatacgga cgggagcacc cgtttgggca tcccgtcatg cggactgtga
      361 accgttccgc atccaaccca agctttgcaa cttcacgttc caggtcatcg atcagtttgg
      421 gcaatacccg ttcgctttcg gtgacagcca acggacaggt gggaagggcc acgcacgcca
      481 ttgaccagcg gcgaacgttg gaaatttcct cactcaaagg aacaccgtaa cgccgcaaga
      541 tctgttcaat ctccgatcgg gccgaggcct ccaaatcgca aaagaggacg ctttgttgcg
      601 gcgtgaatcg aatacttggg tggagtcgtt ggcaaatctc ccggagggca cttttcaaac
      661 ggaaggcgtc ccgatccacg atacgcccat tttcgagatt gaggccataa aagaatcgcc
      721 catcaccttg ctcatgccaa cccatatggt catcaacctc ggtgacaccg agtggtaatg
      781 ggtcgggcaa agaccgacct aggtagcgct ccgtcatctc tcggaaccaa ggtagcccgc
      841 ggtccgcaat gaggtacttg agtctcgccc gcttccggtc gcctcgctca ccataatcgc
      901 gataaatttt gacgaccact tccgccaccg gaaccacctc cgcaggccgg acaaacacca
      961 ggggctgact caaggccggg aaggtatttt tgttactcgg cgttaccccc aagccaccgc
     1021 ctaccagcac attgaaacca actacctcgc cgttttcgac aacggctaga aaaccgagat
     1081 cgttagtgta aagatcaacg cagttatcgt cgggtagccc aaaggcgatc ttgaactttc
     1141 gcgggagata ggttttccca tagatcggct caacctccgc aacctctcct gacggctcgg
     1201 tgacttgctc cttttcaccc gtatccgggt ctgtaagcca aatctcatga taggccggcg
     1261 tccttggcag caggtggcga gtaatctcct gggccaagtt ttgaagttgc tcccgaacac
     1321 ggttgtgccg caatggcgcg gggcagcaca tcacgttccg aaccacgtcg ccacaggcac
     1381 cgagcgtggt tagctgcacc tcattgatcc ggcggatagt ttctcgcaag ttccccttga
     1441 gaacgccgtg caattgaagg tcctgccggt cagtaatgcg tgccgtgccg ttacctagct
     1501 ggtcacaaag gtcgagttga gtaagaaact gttccgccgt cagtacacca cccgggacct
     1561 tcacccgaat catggtgaaa tacacctttc ccccgcccga ggccctttga agctttcggg
     1621 catcacgatc gtcctgctgg tacgagccgt ggaacttaag aatctgggca gccgactcgg
     1681 aaaagctggg ctggtcgttg ttcacctcct ccgcaagggg atgccgaagg tactgacttt
     1741 cctctttcag cttttcgacc tgacttcgct tgagtggttg ggcttcgtgg tccatgcgtg
     1801 catcgttcct ttctcgtcgt atccggagag acaaatttac atcattatgt tgatcacatt
     1861 actgacacat taaattctac agccacaatt gacgccagaa aagacctggc aacggttttt
     1921 tgcagaaaaa ggccgatagc gaaattacgc cctgagatga gaaaacgcgg cacgaggag
//
LOCUS       DAOPTR010000804         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_45050_length_1977_cov_2.630073, whole genome shotgun sequence.
ACCESSION   DAOPTR010000804 DAOPTR010000000
VERSION     DAOPTR010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45050_length_1977_cov_2.630073"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /locus_tag="PKI05_13355"
     CDS             <1..1271
                     /locus_tag="PKI05_13355"
                     /inference="COORDINATES: protein motif:HMM:NF021041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOA53235.1"
                     /translation="EIYAEKLGDQLEFDSPFAGTDREKLEEPLWTPPDLPKVTDPFAA
                     PPPSELVPEGVTSTSDIQAQVIGYALKGRDAGSRQALLGLYGGTKTTEAAVELGLQWL
                     SRQQRKDGSWSLTGPYPNGCETENPEAATALALIAFQGHGNTHKTGPFRDNVSRAWNW
                     LLQQQDANGNFFHEGNFNHPFYTQGLCTIAVCEIYGMTKDERFREPALRAIDYLIKSQ
                     SSEGGWRYTANTDSDVSVTGWIVMALQSARMAGFEVPQDVFRKVERFLDRVAIEGGSK
                     YPYQKGGQASLAMTAEALLMRQFLGWSHNDDRLAKGLAWITEPANLVNFERDRDVYYW
                     YYATQVCHHMEGEYWKKWNEVMRQLIPQHQVKSGPQAGSWDPTKPSRDAWANEGGRLY
                     VTCLSIYCLEVYYRHLPLYSQRALRLVGGM"
     gene            1388..>1977
                     /locus_tag="PKI05_13360"
     CDS             1388..>1977
                     /locus_tag="PKI05_13360"
                     /inference="COORDINATES: protein motif:HMM:NF012691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOA53236.1"
                     /translation="MAHTIVIFGASGDLTRRKLIPALYELHRKKRLPPETTIVGMSRT
                     QYSHDQWREVLREATAAQVGPKLLPDLWDEFARRIYYYPGDVSRAEDFPGLNAFISSL
                     EAEKNTTRVYYLSLAPEFYECTVEHLGQAGMADETHAPRRIVVEKPFGRDLQSARVLN
                     AKIHEVFAEHQIYRIDHYLGKETVANVLVLRFANTI"
BASE COUNT          473 a          526 c          544 g          434 t
ORIGIN      
        1 ccgaaattta tgcggaaaaa ttgggagacc aactggagtt cgattcacca tttgcaggaa
       61 ccgaccggga gaagctggaa gagccgctat ggacgcctcc tgatcttccc aaggtaaccg
      121 atccttttgc cgcaccgccg ccttccgagc tagtccccga aggtgtgact tcgaccagtg
      181 acattcaagc ccaggtcatt ggatacgcgc tcaaggggcg ggacgccgga agcagacaag
      241 ccctgctcgg gctttacggc ggcacaaaaa cgacggaggc cgccgtagaa ttggggctcc
      301 aatggcttag ccggcaacag cgtaaagacg gttcgtggag tcttaccggc ccgtatccca
      361 acgggtgtga aacggaaaat cctgaggccg ccaccgccct tgcgctgatc gcctttcaag
      421 ggcacggaaa cacacacaaa acggggccat ttagggataa tgtctcccgc gcgtggaatt
      481 ggctgcttca gcaacaagac gccaatggca atttctttca tgaaggtaat ttcaatcatc
      541 ccttttacac gcaagggctt tgtactatcg ccgtgtgcga aatttatggc atgacaaagg
      601 atgagcgatt ccgtgagcca gcattacggg caattgatta cctcatcaaa tcacaaagtt
      661 ctgagggtgg ctggcgatac accgccaata cggatagcga tgtctctgtc accggttgga
      721 ttgtaatggc ccttcaaagt gcccgcatgg ccggtttcga ggtgccgcag gatgtcttcc
      781 gaaaagtgga acgcttcttg gaccgtgtcg ccatcgaggg aggcagcaag tatccctatc
      841 aaaagggcgg tcaagctagc ctcgccatga ctgctgaggc gctcttgatg cggcaattct
      901 tgggctggtc gcataacgac gaccgccttg cgaaaggctt ggcttggatc acagaaccgg
      961 cgaatctcgt gaattttgaa cgcgatcggg acgtctatta ctggtattac gccacgcagg
     1021 tgtgccacca catggagggc gaatactgga aaaaatggaa cgaggtgatg cgacagttga
     1081 ttccccaaca ccaggtcaaa tctggcccgc aagccggaag ttgggaccct acaaagcctt
     1141 cacgcgatgc ttgggcaaac gaggggggac ggctctacgt gacatgtctg tccatctact
     1201 gtttggaggt ctattaccgt catttgccct tgtactctca acgggccttg cgactggtgg
     1261 gtggtatgtg ataccgggtc cccttggctg ttgaccgacc tggataataa tagcaacgtc
     1321 aacaaagcgg ttatcggccc atttggcaat cagtcttcgc aggggtcaag gcgtttctgg
     1381 aatagccatg gcacacacaa ttgtcatttt tggagcatcg ggtgatctaa cgcgacgaaa
     1441 gcttatcccg gccctctacg agctgcatcg aaaaaaacgg ttacccccgg aaacaaccat
     1501 cgtcgggatg tccagaactc agtattcgca cgaccaatgg cgtgaggtcc tcagagaagc
     1561 aacggcggcc caggtcggcc ccaagcttct tccagatctg tgggatgaat tcgcacgtcg
     1621 catctactac tatccgggag acgtcagccg ggcagaagat tttcccggct tgaatgcctt
     1681 catttcgagt ctcgaggcag aaaaaaatac cacacgggtt tattatctct cgcttgctcc
     1741 agagttctat gaatgcacgg tggagcatct tgggcaggcg ggaatggctg atgaaacgca
     1801 tgcccctcgg cgcatcgttg tggaaaaacc gttcggccgt gatcttcaat ccgcacgcgt
     1861 cttgaacgcc aagatccacg aggtcttcgc tgagcatcag atatatcgga tcgatcacta
     1921 cctgggtaaa gagacggtgg ccaacgtcct cgtcttgcgg tttgccaata ccatctt
//
LOCUS       DAOPTR010000805         1976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_45068_length_1976_cov_3.102030, whole genome shotgun sequence.
ACCESSION   DAOPTR010000805 DAOPTR010000000
VERSION     DAOPTR010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45068_length_1976_cov_3.102030"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1976)
                     /locus_tag="PKI05_13365"
     CDS             complement(<1..>1976)
                     /locus_tag="PKI05_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53237.1"
                     /translation="PLRFRRVFVPKDVLDQVRDPVPYFPLNAAEFEKMIAAANRQVVF
                     RGQHARIVTAIYRANVRDHDLVGLEGEWLVELFHGTSAILPLGKLNIGLSDPVWVDLG
                     GAPVSLLSDQEGNVSLYVERSGKVGFRWSAHGQTVGAGGSIGFDLTFPPAIRTELELY
                     TSDRFDVAAEGGLVFPMSSSSGGLAGYRLIISDPSRFGMVLQPRRPPAQPVPRGDYRE
                     EIAYELTLQGLEATWQWSIDVVDSPLEGLKIRVPQHVRIMEISLGDVALPWEYIQRND
                     SLTDTIRVRFPEPLTGHNRRVTLRGLTSLQLDGPIVLPTLALEGLFWRSGVSTVIVRM
                     PLAIQDVKLRSARLRDVRTLSAGPLGTVLEVVHDTSGADMTLFCRLTAPKVDETTVVV
                     TKITPSEVQAEVRSALRSDGDGCFELTWPISPAWTILDVSSSDQSLLAGWQFRPGGDK
                     PSKGGALTVRLKKSLPTDKPALLRMRLRRDPPTWDHAVLFDEMIPLVASAMPSTAHLY
                     AALILDPAMAAGRTIEVEPDVEPIGPVELTSLANLYETGSSLQLFDLSELDVKPKVRL
                     VPRQFLYQAQINGKIEINGENLLQSFRVTIEPDQTGVNELCVFWSEVGVDRQFDWFLI
                     ESEGRETPLSVSEQSFSPCHNIKTSVVSLPKTMQ"
BASE COUNT          475 a          562 c          539 g          400 t
ORIGIN      
        1 cttgcatggt ttttggcaaa gaaaccacgc tggtctttat gttgtgacac gggctgaaag
       61 actgttcgct cacggagagc ggcgtttcgc gcccctcact ttcgatgagg aaccaatcga
      121 actggcggtc cacccccacc tcgctccaga agacacataa ctcattaacg ccagtttgat
      181 ctggttcaat cgtcacgcga aatgactgaa gaaggttctc gccattgatc tctattttgc
      241 cgttgatctg agcttgatag aggaattgcc tcgggacaag ccgtaccttg ggttttacat
      301 ccaactccga aagatcgaac aactggagtg aggaacccgt ctcgtaaaga tttgctaacg
      361 aggttaattc caccggccca atgggttcta catcaggttc aacttcgatg gtcctgccgg
      421 ctgccatggc agggtcgagg atcagcgcag cgtaaaggtg tgccgttgac ggcatagctg
      481 aagcgaccag gggaatcatc tcgtcgaata gtacagcgtg gtcccacgtg ggcggatcac
      541 gacggagtcg catcctcaac agggctggct tgtctgtagg aagggatttc ttgagccgca
      601 ccgtgagtgc accacccttg ctcggtttat caccccctgg acggaattgc cagcccgcaa
      661 gcaaggactg atcggatgac gagacatcaa ggattgtcca tgctggcgaa atcggccacg
      721 tcagctcaaa gcaaccgtcc ccgtcagacc gcaaggcgga tcggacttca gcttggactt
      781 ctgagggggt gattttggtt acaaccacag ttgtctcatc taccttgggg gcggtaagcc
      841 tgcagaaaag cgtcatatcc gcacccgaag tatcatgaac gacttccagt acagtcccca
      901 gcggtccagc cgataaggtc ctaacatctc ttaggcgagc ggagcgaagt ttcacgtcct
      961 gaatggccag gggcatcctc acaataaccg tggacacccc cgatcgccag aataatccct
     1021 cgagagccaa cgtgggaagc acaatcgggc catccagttg taggctggtc agacctcgca
     1081 atgtgacccg ccgattatga ccggtcaatg gttcggggaa ccgaacgcgg atcgtatccg
     1141 ttagcgagtc attcctttgg atatattccc acggcagggc cacatctccc agggaaattt
     1201 ccatgatgcg gacgtgttgc ggaacacgaa ttttcaaacc ctccaaggga ctgtcaacga
     1261 cgtcaattga ccactgccac gtagcttcga gaccttgtaa ggtcaactcg taggcgattt
     1321 cttcccgata gtctcctcga ggtacaggct gtgccggggg acgccgcggc tggaggacca
     1381 tcccgaagcg tgatggatcg ctgataatta gccggtaacc ggccaatccg ccggaggagg
     1441 aagacatggg aaacacgagg ccgccttccg ccgcaacatc gaaacgatct gatgtgtaga
     1501 gttccagctc ggtgcgaatc gccgggggga acgtcaagtc aaagccaatg gaacccccag
     1561 cccccaccgt ttgcccgtgc gccgaccaac gaaaaccaac ctttcccgac ctttccacat
     1621 agagactgac gttgccttcc tgatcggaca acaaggagac cggggcaccc ccgagatcaa
     1681 cccagacggg atcgcttagg ccgatattga gcttgccaag ggggagaatc gccgaggtgc
     1741 cgtgaaacaa ttccaccaac cactcgcctt ccaagccgac caggtcatgg tcacgaacgt
     1801 ttgcccgata gatagccgtc acgatgcggg catgttgtcc gcgaaagacc acctgccggt
     1861 tggccgcagc aatcattttc tcgaattccg ccgcattgag aggaaaatac gggaccggat
     1921 cacgaacttg gtcaaggacg tccttgggta caaacacgcg acggaatcgc aggggc
//
LOCUS       DAOPTR010000806         1970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_45267_length_1970_cov_3.231332, whole genome shotgun sequence.
ACCESSION   DAOPTR010000806 DAOPTR010000000
VERSION     DAOPTR010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45267_length_1970_cov_3.231332"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..>1970)
                     /locus_tag="PKI05_13370"
     CDS             complement(6..>1970)
                     /locus_tag="PKI05_13370"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOA53238.1"
                     /translation="WATIIYEKLPDGRKVAHVQAKGVAENVAIRFRSQDGSWDLSDFC
                     YVGIQVQNRLEKEIRVQWRVRAKDKSLSRDYVMKPGESHFLRLTLRRAVPTQLKDVFF
                     GMRGYPALMDPERGIAVNAVEELALNIRGPVEGTFLTIENVAASGKFQKPQWWNGITS
                     RLFPMIDMFGQYRHADWPGKTHSIEELRARIAEEDQDLAQHPGPSDWNQFGGWIGGPK
                     LEAKGHFWVTKHDGRWWLVDPEGCLFWSHGIDCVRATTATTPITDREHWFELPARDGP
                     FGLFYSRAGWAPHGYYQKRSPYEQFCFSGANLLRKYGENWEAAFNERAHRRLRSWGLN
                     TIGNWSDPAIYREKKTPYVVTLSSGKRKIEGSTGYWGQFPDPFDPTFDQPLREAMARE
                     ANQSVIDPWCLGYFVDNELSWGDDVSLALATVRSPAEQPAKKAFMEMLKQKYGTVEKL
                     NEVWGTKYQSWENFLEVTDEPDRKKAESDLGAFYSEIAETYFRRCREAIKAVGPNKLY
                     LGCRFAWVNERAIRAAAKYCDIISFNRYEVSVASFTLPQGVDKPVIIGEFHFGALDRG
                     MFHPGLRETADQVERAEAYRRYVQSALDHPQIVGTHWFQYMDQATTGRGDGENYQIGF
                     LDVCDTPYPEIIQASRDVAAEMYRRRAHHQ"
BASE COUNT          446 a          570 c          486 g          468 t
ORIGIN      
        1 gtcacctatt ggtggtgggc ccgacgccga tacatctcgg cggcgacatc gcgggatgcc
       61 tgaatgatct ccggatacgg tgtatcgcag acgtccagga atccaatctg ataattctcg
      121 ccatcaccgc ggcccgtggt ggcttggtcc atgtattgga accaatgggt cccaactatt
      181 tgggggtgat ccaacgcact ctggacatat cgacgatagg cctcggcacg ctcgacttga
      241 tcggccgttt cgcgcagccc ggggtgaaac atgccgcgat cgagtgcccc gaagtggaat
      301 tcgccgataa tcaccggctt atccacccct tggggaagtg taaaactcgc aacactaact
      361 tcgtagcgat tgaaactgat aatatcgcag tatttggcgg cggcgcgaat ggcccgctca
      421 ttaacccagg caaaccggca accaagatag agcttgttcg gtccaacggc cttaatggcc
      481 tctcggcagc gccggaaata tgtctccgca atctcgctgt agaatgcgcc aagatcactt
      541 tccgccttct tgcgatcagg ctcatcagtg acttccagaa aattctccca agattggtac
      601 ttggttcccc atacttcatt taacttctcc accgtaccgt atttctgctt taacatctcc
      661 ataaaagcct tcttggccgg ttgttctgct ggcgaacgaa ccgtggccaa ggcgagcgac
      721 acatcgtctc cccagctaag ttcgttgtcc acaaaataac ccaagcacca ggggtcgatc
      781 actgattggt tcgcttctcg cgccattgcc tcacgcaaag gttggtcaaa agttgggtcg
      841 aaggggtcgg ggaattgacc ccaataaccg gttgatcctt cgatttttcg ttttccagac
      901 gataaggtca ccacataagg tgtctttttt tcgcggtaga tggccggatc ggaccagttt
      961 ccgatagtat tcagtcccca gctccgcaac cggcgatggg cgcgctcgtt gaaggccgct
     1021 tcccaattct ctccatactt tcgtagcagg ttggcaccgg aaaagcagaa ttgctcatag
     1081 ggggaacgct tttggtaata cccatgcggt gcccatcccg cccgactgta gaaaaggccg
     1141 aatggtccat cgcgagcagg caattcaaac cagtgttcgc ggtccgtgat tggggtcgtg
     1201 gccgtcgttg cacgtacaca atcgatcccg tgagaccaaa acaaacaccc ctccggatcc
     1261 accaaccacc atcggccgtc gtgtttcgtt acccagaaat ggccctttgc ttcgagtttt
     1321 ggcccgccaa tccaaccgcc aaattgattc cagtcgctcg gaccgggatg ttgcgctaaa
     1381 tcctgatctt cttctgcaat tcgggctcgc aattcttcaa tggagtgcgt cttaccgggc
     1441 cagtcagcgt gccggtactg cccgaacatg tcaatcatgg gaaacaaccg gctcgttatc
     1501 ccgttccacc actggggctt ttggaatttc ccagaggcag ccacattttc gattgtcaaa
     1561 aaagtgccct caacaggacc acggatattg agggccagtt cctccaccgc gttgacggcg
     1621 atacctcgct ccggatccat aagggctggg tagccccgca ttccaaaaaa gacgtctttc
     1681 aactgtgttg gtacggccct ccggagagtt aaccgtagaa agtgggactc gcccggtttc
     1741 atgacataat cgcgagacag tgacttatct ttggctcgaa cccgccactg aacacgaatc
     1801 tctttttcga gccggttttg cacctgaatg ccgacgtaac aaaagtcact cagatcccaa
     1861 gaaccatctt gagagcgaaa gcgaatggcc acattttccg ccacgccctt ggcctggaca
     1921 tgagcaacct ttctaccatc tggtaatttt tcgtagataa ttgtggccca
//
LOCUS       DAOPTR010000807         1968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_45323_length_1968_cov_3.151072, whole genome shotgun sequence.
ACCESSION   DAOPTR010000807 DAOPTR010000000
VERSION     DAOPTR010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45323_length_1968_cov_3.151072"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PKI05_13375"
     CDS             complement(<1..190)
                     /locus_tag="PKI05_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53239.1"
                     /translation="MIKPPGDGSDEGRTSSAHLFEPSLWHPTMLSAGGCFDVGLRPLG
                     WGDFSRRGERLELMACVRA"
     gene            692..1147
                     /locus_tag="PKI05_13380"
     CDS             692..1147
                     /locus_tag="PKI05_13380"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOA53240.1"
                     /translation="MRAALVVAIVLLGMNPIGRGSEPVSYSEQFAYSAAYRQSLETQQ
                     PFLVIVGAQWCPACRKLESEVIPKIKEMGLTKQVAFAVVDYDAETKVAQQLTRGGPIP
                     QVLVFRRGEQGWKSDRLVGYPSQKAVISFIQAALSSKDQAPAGQQASNN"
     gene            1204..>1968
                     /locus_tag="PKI05_13385"
     CDS             1204..>1968
                     /locus_tag="PKI05_13385"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOA53241.1"
                     /translation="MLPPIERHNIRQLEELNQRGGRMLTVVDLIEAGTISPEMAAAVM
                     EALARGASILTGARPSGAGKTTVLAAFLNLLPPDVTIVTVDDPGVVGQALARPPEHPT
                     CYLVHEIGAGHWYGYLWGQAVSDYLRLTRGPHRIASCLHADTLEELTHILTSPPLCVD
                     PSDLHRVGILGFMRMDNTRRGGIVRRLATLEIRSPFLDQACRFVFAHSTNSFSREPLC
                     AANDHPSTDATPTSNASGTNINEKAFLVFIEHLLHVD"
BASE COUNT          436 a          571 c          501 g          460 t
ORIGIN      
        1 cggcccttac acaggccatc aactcaagtc tttcgccgcg acgggaaaaa tcaccccaac
       61 caagaggacg taatccaacg tcgaaacagc cccccgccga taacatcgtc ggatgccaaa
      121 ggctcggctc gaaaagatgg gcactcgacg ttcggccttc atcgctcccg tcccctggtg
      181 gtttgatcac cctccaaccg ccgctcgcgg ggtccacttt tattatatcg aaaatccccc
      241 cgaatggcga agtggaaacc agacattttg cctttcgaat atgtccatga atttgacacc
      301 gctgcgtcgg atttggtttc gatttttcat tggcttggta acatcttacc ggagttaaag
      361 ccatccgcgt cagccaagat tgtctccaca tctacagcgt catgcgactg gtatcctacc
      421 cgacatagag ccttccaact tgggcaagac aggaaaacct atttggtcag gcaaagtatt
      481 ccgaaaacgt tgaacctact tgtcttggca aaattgtcgg ttacactaga acacttcctg
      541 ccaaaccgtt gaccggtttg aaatcacccg catgatgcac aggcaagccg accttgccta
      601 tcgaaacgga catcctccct tgcgttgttt cttgaagggc tttgcccacg catcgtttgg
      661 cgaaaggact gttgagagga ggagtgtttc catgcgtgcc gccttggttg ttgcgatcgt
      721 gttattgggg atgaatccga ttggccgtgg ttcggagcct gtttcctatt ctgagcaatt
      781 tgcctattct gctgcgtatc ggcaaagcct cgaaacccag caaccctttt tggtcattgt
      841 gggagcacag tggtgtcctg cttgccgaaa actcgagagt gaagttattc ccaaaatcaa
      901 agagatgggc cttacaaagc aggttgcctt tgctgtggtg gattacgacg ccgagaccaa
      961 ggtggcccag caactcaccc ggggtggccc gattccccag gtgctcgttt ttcgccgcgg
     1021 agagcaaggc tggaaatcgg atcggctcgt cggatatcct tcacaaaagg cagtcatctc
     1081 gttcattcag gcggccctgt cgtcaaaaga tcaagcgccg gccggccaac aggcaagcaa
     1141 caactgagca gacttttgct cttggccgga atagagaccc tcggcatagg caagcacaag
     1201 acgatgcttc caccgatcga acgacacaac atccgccaac tggaagagct taatcagcgc
     1261 ggcggccgta tgctgacggt tgtggatctg attgaggctg ggactatctc gcccgaaatg
     1321 gcagccgccg ttatggaagc cttggcccga ggggcctcga tcctgacagg tgcgcgtccg
     1381 agcggtgcgg gcaagacgac cgttctggcc gcctttctca acctactccc gccggatgtc
     1441 accatcgtca cggttgatga cccgggcgtc gttggacagg ctttggcacg gccacccgaa
     1501 catccgacct gttatctggt ccacgaaatc ggcgccggcc actggtatgg ctatctttgg
     1561 ggacaagccg tctctgacta cttgcgcttg accagaggtc ctcatcgcat cgcctcgtgc
     1621 ctccatgccg ataccctgga ggaactcact cacatcctga ctagcccacc actttgtgtc
     1681 gatccatccg atctgcatcg ggttggtatc ttgggattta tgcggatgga caacacacgc
     1741 cgagggggta ttgtccgccg gttggcaacg ctggagatcc gctcgccttt tctggatcaa
     1801 gcgtgccgtt tcgtttttgc tcattccaca aactctttct cgagagaacc gttgtgtgcg
     1861 gcgaatgatc acccatcaac cgatgccaca ccaacctcaa atgcctctgg cacaaacatc
     1921 aatgaaaagg cctttctcgt tttcatcgaa cacttactcc acgtggat
//
LOCUS       DAOPTR010000808         1967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_45343_length_1967_cov_4.770921, whole genome shotgun sequence.
ACCESSION   DAOPTR010000808 DAOPTR010000000
VERSION     DAOPTR010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45343_length_1967_cov_4.770921"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1019)
                     /locus_tag="PKI05_13390"
     CDS             complement(54..1019)
                     /locus_tag="PKI05_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOA53242.1"
                     /translation="MPRKVIVDLDPGIDDALALCMLLFDPDWDVVAVTAVGGNVPPHV
                     ATRNAQALIAYLDPPRIPRIGAATAPERGLPVDGRFANSVDDLRDASLTVAELRTPHP
                     SEKLLTDEVRAAPHAVTVLALGPLTNIARAIQRDPEFPVLVHRLIIAGGAIQVPGNIT
                     PAAEFNIYCDPHAAKIVFASKAAKTLVPLDVTNRVNFSLSFLNELPPETNKIGALLRK
                     ILPAKFRAYRQHLGLETIHLHDSLTYLAACRPDLFRYEELAGDVETVGEITRGATIFD
                     RRPVPAWQENRLEVAVGIDAAAIRLELLKRLNAAAQAAGNVESPP"
     gene            1137..>1967
                     /gene="trpD"
                     /locus_tag="PKI05_13395"
     CDS             1137..>1967
                     /gene="trpD"
                     /locus_tag="PKI05_13395"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOA53243.1"
                     /translation="MLEAAVAKAERGANLTMEECESAVEAIIRGQAPEEVVARFLIAL
                     HVKGETAAEIAGAARAMRRHMLTVESSHPIVMDIVGTGGDGSGTFNISTAAAIVIAAA
                     GVPVAKHGNRRITSRTGSADVLAELGINIEAPLPVVTACLEELGICFCFAPLMHPAMK
                     NVAEVRRKLGHPTVFNLLGPLTNPASVPYQVLGVGRTFLRPLLAEALQLLGTTRSAIV
                     YGCGELDEVSLLGETQATIVGPDGVEEVIWKPEDFGLEPISCEALRVSGPGESAARIK
                     E"
BASE COUNT          414 a          500 c          643 g          410 t
ORIGIN      
        1 cggaatgtga aaactgctct caactgtccc aacggagtgg gggaatcgat gcgtcacgga
       61 ggtgattcca cattgccggc tgcctgggcc gccgcgttaa ggcgtttgag caattccagg
      121 cggatggcag cagcatcgat gcccacggcg acctcaagcc ggttttcctg ccaggcaggg
      181 acagggcggc ggtcaaagat ggtcgctccc cgggtgattt cgccaactgt ttccacgtcc
      241 cccgccaatt cttcgtaacg gaagaggtcg ggacggcagg ccgccaaata agttagcgag
      301 tcatggaggt ggatggtctc tagccccaag tgttgccgat acgcccgaaa ctttgccggc
      361 aaaatcttgc gcaacaaggc gccaatcttg ttcgtttccg gcggcagttc gttcaaaaag
      421 gaaagggaaa agttgacgcg attcgtcacg tccaggggga caagggtctt agcagccttg
      481 gaagcgaaaa cgatcttggc cgcgtgagga tcgcagtaga tattgaattc cgccgcaggc
      541 gtaatatttc cggggacttg aatggctccg ccggcaataa tcagtcgatg gaccaacact
      601 ggaaactcgg gatcacgttg gatggcacgg gcgatgttgg tcaggggacc gagggcgagc
      661 acggttaccg catggggggc cgcccgaact tcgtcggtca aaagtttctc cgaaggatgg
      721 ggggttctta gctccgcgac agtaagagac gcgtctcgaa gatcatctac cgaattggcg
      781 aaccggccat ccacagggag acccctctcg ggtgcggtgg ccgccccgat tcttggaatt
      841 cgcgggggat ccaggtatgc tataagtgcc tgagcgttcc gagtggcaac gtgcgggggg
      901 acatttcctc caactgctgt gaccgccacg acgtcccaat caggatcaaa gaggagcatg
      961 cataatgcca gcgcgtcgtc gattccagga tcgagatcaa ctatgacttt tcgtggcatc
     1021 gcttaccctc gtcaggtggg gtcaattcct atttgacagt gggatatggc ccgattttag
     1081 gtttccttgc tcatcgtcgc aagggcatgt ggtcgtcagg agaaagggaa aatgccatgc
     1141 tggaagctgc cgttgcaaaa gcggaacgtg gggcaaattt gacgatggaa gagtgcgaaa
     1201 gcgcggtgga ggccatcatc cgaggccagg ctccggaaga ggtggtcgcg cgcttcttga
     1261 tcgcgcttca cgtcaaaggc gagacggccg cggagattgc cggcgctgcg cgcgcgatgc
     1321 gccgtcatat gctcaccgtc gagagtagcc atcccatcgt catggatatc gtggggaccg
     1381 gcggtgacgg ctcggggacg ttcaacatca gcacggcggc ggcgattgtt attgcggcag
     1441 cgggcgttcc ggtggccaag catggaaatc ggcggattac gagtcggacg ggctccgcgg
     1501 atgtgttggc ggagttgggg atcaatatcg aggccccgtt gcccgtagtg actgcatgcc
     1561 tggaagagct gggcatctgc ttctgttttg ctccgcttat gcatccggcc atgaaaaatg
     1621 tggcggaagt acgtcggaaa cttggtcatc ctactgtgtt taatctcttg gggccgctca
     1681 cgaacccggc gagtgttcct taccaagtgc tgggtgttgg ccgaacgttt ttgcgtcccc
     1741 ttctggctga ggccctgcaa ctattgggga cgacgcggag cgccatcgtc tatgggtgtg
     1801 gcgaactgga tgaggtgagc cttctgggag aaacgcaggc caccatcgtg ggaccagacg
     1861 gtgtagaaga agttatttgg aaaccagaag atttcggact ggagccgatt tcttgcgagg
     1921 cgttgcgggt aagtggaccc ggagaaagtg ccgcccgaat taaggag
//
LOCUS       DAOPTR010000809         1967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_45351_length_1967_cov_3.740063, whole genome shotgun sequence.
ACCESSION   DAOPTR010000809 DAOPTR010000000
VERSION     DAOPTR010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45351_length_1967_cov_3.740063"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="PKI05_13400"
     CDS             <1..890
                     /locus_tag="PKI05_13400"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HOA53244.1"
                     /translation="ANFSRLLTLHRDEAGAVQSLSFVLTLPFFVLLMLFIIQVSQIMI
                     GTVVVHYAAYAAARAAIVWIPAKVGDWELENCIGPGYVLDPNAPDQVIPITDPTSSEY
                     GPTSGGLTFIIPYDPASPKYQKIVNAAILAVMPICPSRDLRLAVSGSVGTAAQLLQQA
                     YFANVPGASSIPRIPRRLENKLAYARLATELELRFFHSNREPPLAPYFLEDDIYEFRP
                     NELGFQDTVTVTLTHHMALLPGPGRFLARPVRRTDEVAEQIERRNGIATYPLRASISM
                     GLEGEKSVIPYTYSIVAFW"
     gene            1011..>1967
                     /locus_tag="PKI05_13405"
     CDS             1011..>1967
                     /locus_tag="PKI05_13405"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HOA53245.1"
                     /translation="MLSKGDVRTIAVMRKCAARLGCRLRSLSADQDGAMSVVSVFAVL
                     LLTILLGMVMNVGRVVDHKIRLQNAADAVAYSGGVVIARGMNALAFTNHLLCDVFALT
                     AYMREARDRHAESYVPAILDAWNQEAPVFAQSNFEKFIPLASAIPAKTPLEQALVDGF
                     CSWAAAASQVVLPTLEFILQEELIPEYQRAVVAAFPDIAQQAAMEVARRNGRPDFGRG
                     EMLGVLWRTNVVPVGGPGEFVQRTLPVVDPVMDQFPNQADYFNTAVEQRRNLSHHYLR
                     LWNNRSLIFFDREAKMSQFASLWRSFTCGQLEQLLSEYPASNL"
BASE COUNT          389 a          501 c          580 g          497 t
ORIGIN      
        1 aagccaattt cagccggctc ttaactctcc accgtgacga agccggggct gtgcaaagcc
       61 ttagctttgt ccttacgctg cccttctttg ttctattgat gctctttatt atccaggtca
      121 gccagatcat gatcggcacg gtcgtcgtcc attatgcggc gtatgctgcc gcgcgggcgg
      181 ctattgtgtg gatcccggcc aaggtgggcg actgggaatt ggaaaactgc atagggcccg
      241 gctatgtgtt agatccgaac gccccggatc aggtcattcc catcacagat ccaacttcat
      301 ccgaatacgg acctaccagt ggtggtttga cgtttatcat tccttatgat ccagccagcc
      361 ctaaatatca gaaaattgta aacgccgcca ttttggcggt gatgcccatt tgcccctcac
      421 gggatttaag gctcgcggtg tcgggttccg ttggcacggc tgcccagctt ttgcagcaag
      481 cctatttcgc caatgtgccc ggggcctcga gcataccacg gataccgcgg cggttggaaa
      541 ataagctggc ctatgcccgg cttgcgacgg agttggaact ccgctttttt cactcgaatc
      601 gcgagccacc cctcgcccca tattttctcg aagacgatat ttacgaattc cggcccaatg
      661 aattgggatt tcaggatacg gtgacggtga cactgacgca tcatatggcg cttttgcccg
      721 ggccgggccg attcctggcg cgaccagttc gccggaccga tgaggttgcc gagcagattg
      781 aacgtcggaa tgggattgcc acctatccgt tgcgggcgtc catttcgatg ggcctcgaag
      841 gagaaaaatc ggtaatcccc tacacatatt cgattgtcgc cttctggtga ggaagttgat
      901 tccttgacga aggtcggctg aactgccgtt atgtgtgatt tgtaagtgtt gtgggttctc
      961 aggctgcgcg gaaacggtat cgaggaaagg cgtgctgccg gcgaaaaaat atgctctcaa
     1021 agggtgatgt gcgaacaatc gccgtgatgc ggaaatgtgc ggcgaggctg ggatgtcggt
     1081 tgcgatcgct ttctgccgat caagatgggg cgatgagtgt cgtttcggtt ttcgccgtac
     1141 tcttgctcac tatcttgctt gggatggtca tgaatgtggg gcgggtggtg gaccataaga
     1201 tccgcctcca gaatgcggcc gatgctgtgg cctattccgg gggagtggtg attgcccgcg
     1261 gcatgaacgc cttggccttc accaatcacc tcctttgtga tgtctttgcc cttacggcct
     1321 acatgcggga agcaagagac cgacacgctg agagttatgt cccggccatc ttagatgcct
     1381 ggaatcaaga ggcgcccgta tttgctcagt caaattttga aaagtttatt ccactggcat
     1441 cggccatccc tgcaaagacc ccgctagaac aggctttggt ggacggtttt tgcagttggg
     1501 cggctgccgc tagccaggtg gttcttccta cgttggagtt cattcttcaa gaggagctga
     1561 ttcctgagta tcagcgggcg gtggtggcgg cctttccgga tattgcccag caggcggcca
     1621 tggaagttgc caggcggaac ggcagacccg attttggccg gggggagatg ttgggtgtcc
     1681 tttggcggac aaacgttgtt cccgtcggtg ggccgggcga atttgtacaa aggacgctgc
     1741 cggtcgtcga tcccgtaatg gatcagtttc ccaatcaggc cgattatttc aacacggccg
     1801 tggagcagcg acggaatctg tctcatcact atctgcggtt gtggaataat cgttcgctga
     1861 tttttttcga ccgcgaggcg aagatgagtc aattcgcctc gttgtggcgg agctttacgt
     1921 gtgggcaatt ggaacaactg ttgagcgaat acccggcaag caatttg
//
LOCUS       DAOPTR010000810         1965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_45417_length_1965_cov_2.894764, whole genome shotgun sequence.
ACCESSION   DAOPTR010000810 DAOPTR010000000
VERSION     DAOPTR010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45417_length_1965_cov_2.894764"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PKI05_13410"
     CDS             <1..577
                     /locus_tag="PKI05_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOA53246.1"
                     /translation="ERFKNHGVSACAVCDGALPRFRNRPVAVIGGGDTAVEEATYLSH
                     WASKVYLVHRRDQLRASKVMAERALSNPKIEVLWNRVVDEVLGDDENGVTGIRLKSTV
                     GEPDLTIDVAGMFLAIGHTPNTAFLKGQLELTEKGYIKLTRPFRTYTSVEGVFAAGDV
                     ADDYYRQAVTAAASGCMAALDVERWLAGQGR"
     gene            757..>1965
                     /gene="asnS"
                     /locus_tag="PKI05_13415"
     CDS             757..>1965
                     /gene="asnS"
                     /locus_tag="PKI05_13415"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOA53247.1"
                     /translation="MEKISVAEARREDAIGREVLLQGWVRTRRDSKGGFSFLEINDGS
                     CLGNIQVVADGTLPNYESEVKRLTTGCSVAVEGVVRASPGREQPTEVYATRVTVYGWA
                     DPETYPLQKKRHSYEFLRTIAHLRPRTNSFGAVTRVRNAVCFSIHRFFQEEGFLYIHT
                     PIITASDCEGAGAMFRVTTLDPATQQRLDDAADFFGRRTYLTVSGQLEAEAYACAIGK
                     VYTFGPTFRAENSNTTRHLAEFWMVEPEAAFFDLDDNMNLAERFLKRIFRDVLEQCPE
                     DMEFFNRWVDNTVIETLRQIIESEFVRLTYTEAIEILKKSGETFEFPVEWGRDLQAEH
                     ERFLTEKHFRRPVILTDYPRQIKPFYMRLNDDEKTVRAMDVLVPRVGEIIGGSQREER
                     LDVLERRMEEM"
BASE COUNT          436 a          486 c          606 g          437 t
ORIGIN      
        1 ggaacgtttc aagaatcatg gtgtgagtgc gtgtgccgtc tgtgacgggg cgctaccgcg
       61 ttttcggaat cgtcccgtgg ccgtaatcgg cggcggtgat accgcggtgg aagaggctac
      121 ctatttatcg cactgggcat cgaaagttta tctcgtccat cgccgcgatc aactgcgggc
      181 gtcgaaggtg atggccgagc gggccttaag caatcccaag atcgaagtcc tctggaaccg
      241 cgtcgtcgat gaagtgctag gagacgacga gaacggcgtt acaggaattc gcctcaagag
      301 tactgttggg gagcccgatt tgacgatcga cgtggcgggc atgtttctgg ccattggaca
      361 cacgccaaac acggctttct tgaaaggaca gttggaactg acagaaaagg gttatatcaa
      421 gctgacccgg cctttccgga cgtacactag tgttgaagga gtctttgcgg cgggcgacgt
      481 ggccgatgat tactatcgtc aagcggtcac ggcggcagcc agtggttgta tggcggctct
      541 ggatgtggaa cgttggctgg cggggcaggg gaggtaggct cttgcgcaaa gcggcccgat
      601 ttcgcatcct cgccgtgaag ccggcaacgt atgtaccgcg aattttttgg gggccagggt
      661 tagccgtaat ggtgatcgcg tgcccgatga ggaaggttta ggggctatag cagacgagtt
      721 ctcgagccta gatcgtctgg gaaaatggtg aagacgatgg aaaagatttc agtagctgag
      781 gcacgacgcg aagacgccat tgggcgcgaa gtccttttgc agggatgggt ccgaacacgg
      841 cgtgattcaa aaggtggttt cagcttcttg gagatcaatg atggttcgtg tctcggaaac
      901 atccaagtag tggccgacgg gacgttgcct aactacgaat cggaggtaaa acgcctgaca
      961 accggctgca gcgttgccgt ggagggggtt gtgcgggcat ctcccggtcg tgaacagccc
     1021 acggaagttt acgcaacccg cgtgaccgtt tatggctggg ccgatccgga gacctatccc
     1081 cttcagaaaa aacggcattc ctacgaattc ttgcggacaa tcgcccattt gcggccccgg
     1141 accaattcct ttggagcggt gacacgagtc cgcaatgccg tctgtttttc gatccatcgt
     1201 tttttccagg aggagggttt cctttacatc cacacgccca tcatcacggc ctcagactgc
     1261 gaaggggcgg gggcaatgtt tcgcgtgacg acactcgatc cggcaacgca acaacggctc
     1321 gatgacgctg cggatttctt tgggcgacgt acatatttaa ccgtcagtgg acagttggaa
     1381 gccgaggcct acgcctgcgc gatcggtaag gtctatacgt ttggtccaac ttttcgtgcc
     1441 gaaaactcga ataccacccg acatctcgcc gagttttgga tggtagagcc ggaagcggcc
     1501 tttttcgacc ttgacgataa tatgaacttg gccgagcggt tcctcaaacg gatctttcgg
     1561 gatgtcctgg aacaatgtcc cgaggacatg gagtttttca accgctgggt ggacaacacg
     1621 gtgattgaaa cgctccgtca gatcatcgag agtgaattcg tccggttgac ctacacggag
     1681 gctatcgaga ttctcaaaaa atctggggaa acgtttgaat ttccggtcga gtggggtcgc
     1741 gatctccagg cggaacacga acggtttttg acagaaaagc actttcggcg gcccgtcatc
     1801 ctcaccgatt atccccgcca gatcaagcct ttttacatgc ggctaaatga cgacgaaaaa
     1861 acagtccggg ccatggatgt gcttgtgccc cgtgtggggg aaatcattgg tggaagccag
     1921 cgggaggagc ggcttgatgt cctcgaacgc cgcatggaag agatg
//
LOCUS       DAOPTR010000811         1963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_45481_length_1963_cov_2.368973, whole genome shotgun sequence.
ACCESSION   DAOPTR010000811 DAOPTR010000000
VERSION     DAOPTR010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45481_length_1963_cov_2.368973"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1714)
                     /locus_tag="PKI05_13420"
     CDS             complement(<1..1714)
                     /locus_tag="PKI05_13420"
                     /inference="COORDINATES: protein motif:HMM:NF016915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOA53248.1"
                     /translation="MDRCVLRRCWTVLLTAILAVTCGCALRHGGHVRPWAKPGLFLRP
                     QMLSQSPTSLTSRTIQFLRQHNLDSSLHEDPRTLLQQVQAVIQKEPQADNVYAYAEIS
                     YVLAKRVERSQPRLALDFYGGTVLKAYQYLFDPKFEDTRNPYDPRFRGACDLYNSALE
                     AALRIVRANESLQPGSAFSVRTADGYWTLTCTICDRRWRAEDFGTFEFVSDYRITGLR
                     NHHVHYGLGVPMIAVRKNYPGEPPAARYYPPDLSFPVTVFLRPEIPSATESQEEDRHY
                     EGRLELYDPVATMNIQVGNYVVPLQADLTTPLATFLSNPALGSSLATVGLLRPEKLLT
                     LQPGLNKPLTGLYMVQPYEPDKIPVIMIHGWWSSPMTWIQMFNDLRSDPLIRRRYQFW
                     FYLYPTGQPFWISAAQFRRDLRELRQTLDPDHRQPALDQIVLIGHSMGGLIAELQTID
                     SGQDFWKLVSEHPFEEIRTDPISREELREIFFFQPNPSIRRIITIATPHLGSHFSNQM
                     TQRLAARLISVPKELLQSQTQFFRENQDLLLPNNILQITDSVDALDPDCPIFALMNQA
                     RRASW"
BASE COUNT          465 a          494 c          596 g          408 t
ORIGIN      
        1 tccagcttgc ccgccgagct tggttcatca gagcaaagat ggggcaatcg ggatcgaggg
       61 catccacgct gtcggtgatc tggagaatgt tgttaggaag gagaaggtct tgattttccc
      121 ggaaaaattg agtctgggac tggagaagtt ctttcggcac acttatcaag cgggcagcta
      181 gccgctgggt catttgatta gaaaaatgtg atcccaagtg cggtgtggcg atggtgatga
      241 tccgccgtat cgacggattg ggctggaaga agaagatttc gcgcaattcc tcacgcgaaa
      301 tagggtccgt gcgaatttcc tcaaaaggat gttcgctcac caatttccag aagtcttggc
      361 cactatcaat ggtctgtagt tcggcgataa gccctcccat gctgtggcca atcaggacga
      421 tttggtcgag ggccggttgc cgatggtcag gatcgagcgt ctgacgtaat tcccgcaagt
      481 cgcggcgaaa ctgtgcggcg gaaatccaaa atggctgacc tgtgggatag agatagaacc
      541 aaaactggta acgtcgccgg atcagcggat cgctacggag atcgttgaac atctggatcc
      601 acgtcatggg gcttgaccac cagccgtgaa tcatgatgac ggggatctta tccggctcgt
      661 atggctgaac catgtagaga ccggttaagg gtttgttcag acccggttgg agagtcaaca
      721 gtttttccgg ccgcagtagc ccgaccgtgg ccagagacga tcccaaggcc ggattagaca
      781 ggaacgtggc caggggtgtg gtcaggtcgg cctggagggg aacaacatag tttccaacct
      841 gaatgttcat cgtggccacg gggtcataaa gctccaaccg gccttcgtaa tgacggtctt
      901 cctcttggga ctctgtggca gagggaatct ctggtcggag gaacaccgtc acaggaaaac
      961 tcaagtcggg gggatagtag cgagcggcgg gcggctcgcc cggatagttc ttccgcacgg
     1021 cgatcatggg cacccccagg ccataatgga cgtggtgatt tcgtagcccg gtgatccgat
     1081 aatccgaaac gaattcaaat gttccaaaat cttcggcccg ccaccgacga tcgcagatgg
     1141 tacaggtgag tgtccaatag ccgtcagccg tacgaacgga aaaagcactt cccggctgaa
     1201 gtgattcatt ggcccgaacg atgcgtaagg cggcttccaa ggccgaattg taaaggtcgc
     1261 acgccccacg aaaccgcggg tcatacggat ttcgcgtgtc ctcaaacttg gggtcgaaca
     1321 ggtactggta ggcctttaaa accgtgccgc cgtaaaaatc gagggccaac cgcggctggg
     1381 accgttccac ccgttttgcg aggacatagc taatctcagc ataggcgtaa acattatcgg
     1441 cctgtggttc cttttgaatg accgcctgaa cctgctgaag gagtgtccga gggtcttcat
     1501 gcaaggagga atcgagattg tgctgccgca gaaattggat ggtccgcgaa gtcagtgacg
     1561 tgggggactg gctgagcatt tgcggtcgca ggaatagtcc cggtttcgcc cagggtcgta
     1621 catgtcctcc gtgtcttagc gcgcagccac aggtgaccgc caagatggca gtcagcagca
     1681 cagtccaaca gcggcggagg acacaccggt ccatccagaa ccacctttcc gattgcccgt
     1741 gtcgtcttcc cgagttggcc aactcccctg ggcaagcgcg cagaaagcca gccctcagag
     1801 gcaagcctga gtggggaaag catataccta aagcacacga caatcgtcaa tggcaaacga
     1861 tgagagtgcg atatccaggg aaaatggggg cagtggggga ttggccgggt tttcgtacta
     1921 ctggtccaac gaattctgtc ttgatcgaaa aagggagcca ggc
//
LOCUS       DAOPTR010000812         1961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_45521_length_1961_cov_3.415005, whole genome shotgun sequence.
ACCESSION   DAOPTR010000812 DAOPTR010000000
VERSION     DAOPTR010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45521_length_1961_cov_3.415005"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1914
                     /locus_tag="PKI05_13425"
     CDS             <1..1914
                     /locus_tag="PKI05_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53249.1"
                     /translation="DRLAVETTLRGVVEHIQQPMYEDLVIKVLCKPPRMAKTPGAAGP
                     GGYPDMSPPASEYGSPYPSPDASMYESSTTGEYGSGYPGMAGGPQAKMRGDEIQRLLF
                     DLAKDVLTQKIRMALAAHVMDPQTRQADRQLMMPILTQPVPENLPVLLVFLQKGDSKQ
                     PLVMWIMDTLTDVMAAAFAAILGAEDPNGLNDLLALAQQRQIPGLQRGMPGQGGYPSP
                     EGEYGSVPPGYAPGSPTTVATLPEMGAYPGASPPGPEYSSETSVGPYPGQPGMRPGLS
                     GGVMTTPQGLPKLVAAERRLAVRGLPRDVALKVAPILWGPDLINFVDSRLMQARSLKD
                     NPREILMAATLPRDATRARLASLFRANLENGPEPLAALGFGNNLFVDPGLVVVVKSLP
                     REDLPKPSTSPTTPRTLGRRPPMGRPGQYGQPGARPYPGGETDQYSPADSPYGGGPGG
                     AQSQLTLTPKMRWMLMSESLSRSICKKLKEAGDRLATIDPASLDTTSQKRPPEFQALH
                     PNASVISEYHLDWPNSQLKQELPNVQFDPMRVHYIRLESHSNPNVVAGFYRRKLSTIN
                     QRTIADGIWLDGLQTLTDGKPRRQSIDIFITHDAGEIGPQDQVDVVVELLSVEILSRE
                     AAAARIGGDTALR"
BASE COUNT          432 a          555 c          590 g          384 t
ORIGIN      
        1 gatcgccttg ccgtggaaac gaccctccgg ggcgtcgtgg agcatatcca gcagccaatg
       61 tatgaggacc tagtgattaa ggtattatgc aaaccgcccc gcatggcgaa gacaccaggt
      121 gcagcgggcc cgggtggcta tcctgatatg tcgcctcccg cgtcggaata cggcagtccc
      181 tatccgtctc cggatgcgtc catgtacgag tcgagtacga ccggcgaata tgggtcgggg
      241 tatcccggta tggccggggg accgcaagcc aagatgagag gcgacgagat tcaacgcctt
      301 ttgttcgatc tggccaagga cgtgcttaca cagaaaatcc gcatggcgtt ggcggcgcac
      361 gtgatggatc cccagacccg acaggctgat cggcagctta tgatgccgat ccttacgcag
      421 ccggttcccg aaaatctgcc tgtgttgctt gtgttcctcc agaagggaga cagcaagcaa
      481 ccgcttgtca tgtggatcat ggacacactg actgatgtga tggcagcggc ttttgcagcc
      541 attttggggg cggaagatcc caacggcctg aatgacctgt tggccctggc gcagcaacgc
      601 cagataccgg gtttgcaaag aggtatgccg gggcaagggg gctatccatc accggagggg
      661 gagtacggat cagtgccgcc gggttacgct cccggtagtc ccacgacggt tgcaaccttg
      721 ccggaaatgg gcgcgtatcc gggggcctca ccaccgggcc cggagtattc ttcggagaca
      781 tctgtggggc cttatccagg acaacccggc atgcgaccgg gactgtcagg cggtgtgatg
      841 acaaccccgc agggcctgcc gaagctcgtg gcagcggaaa gacggttggc tgtccgtgga
      901 ctccctcggg acgttgcttt gaaagttgca cccattctgt gggggcctga tctgattaac
      961 ttcgtcgatt cacgactgat gcaggcccga tcgttgaaag acaatccgcg ggaaatcctg
     1021 atggcggcaa ctctgccacg ggatgcgacc cgtgcacggt tggcttcgtt gttccgggca
     1081 aatctcgaga atggtcccga accgctggct gcccttggtt tcggcaacaa tctgtttgtc
     1141 gatccaggat tggtggtggt agtgaaaagc ctgccccggg aggatcttcc aaaaccgagt
     1201 acctcgccca caacaccgcg gacactaggc cgacgcccac cgatgggccg accggggcaa
     1261 tacgggcaac cgggggcaag accctatccg ggcggggaaa cggatcaata ttcgccggca
     1321 gactcgccct atggcggtgg gccgggcggg gctcagtcgc aactaaccct caccccaaag
     1381 atgagatgga tgctcatgtc cgaaagcctc agccgctcca tctgtaagaa actgaaagaa
     1441 gcaggggatc ggctggcaac gatcgatccc gcttcgcttg ataccaccag ccaaaaacga
     1501 ccgccggagt tccaggctct tcatcccaac gccagtgtca tctcggaata tcatttggat
     1561 tggcctaata gccaattaaa gcaggaactc cccaatgtgc agtttgatcc catgcgggtc
     1621 cactacatcc gcttggagag ccatagcaac cccaatgttg tggcgggctt ctaccggcga
     1681 aaactttcaa cgattaacca acgaacgatt gccgatggga tttggctcga tgggttgcaa
     1741 actctgacgg atggaaagcc gcgacgacag tccatcgata ttttcatcac acacgatgcg
     1801 ggcgaaatcg gtccccagga tcaggttgac gtggtcgtgg aattgttatc ggtagagatt
     1861 ctttcgcgag aggccgctgc cgccaggatc ggtggcgata ctgccctccg gtaatcggct
     1921 atcgtacgga aaacgtcgag aacccaacat cacgcgaaag c
//
LOCUS       DAOPTR010000813         1959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_45584_length_1959_cov_3.556723, whole genome shotgun sequence.
ACCESSION   DAOPTR010000813 DAOPTR010000000
VERSION     DAOPTR010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45584_length_1959_cov_3.556723"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..>1959)
                     /locus_tag="PKI05_13430"
     CDS             complement(71..>1959)
                     /locus_tag="PKI05_13430"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOA53250.1"
                     /translation="SYPDQVIVVRVTADKPGSVGLKASLKTPHVEHAIQTASNAIILR
                     GKVSDYIDKRTKETMPSAIRFEVRALVQPEGGSLVTHDEALQVSGAHAVTILLTAATN
                     YRDFEDVSADPSARCAEILEKARTRSYEELLARHMADYRSLFDRVQFALPSTEAAQQP
                     TNLRIRNFDKQDDPELCVLYFQFGRYLLISSSRPGTQPANLQGIWNESLSPPWESKYT
                     ANINVEMNYWPAEVCNLSECAEPLFDAIEEIAISGARTAKVHYDAPGWVLHHNFDLWR
                     GTAPINAANHGIWPTGGAWLCQHIWWHYEYTQDRDFLAKRGYPLMKGACQFFMHYLVE
                     DPRSPQRWLISGPSNSPEQGGLVMGPTMDHQIIRYLFATTAKAAEILGVDADFRKRLM
                     EMHARIAPNQIGEFGQLQEWLEDKDDPNNKHRHLSHLWGLFPGEEITLRGSPELCQAA
                     RRSLEFRGDGGTGWSKAWKISLWARLADGDRAYKLLSELISQSTLPNMLDNCPPFQID
                     GNFGGTAGIAEMLLQSHGGEINLLPALPAAWETGSIAGLRARGKVGVSIRWDKQSLEA
                     QLNPEVPQVIRLRVPEKFTKVTIFDERGVPVEVGVTEDGARTFSAKAQTYIVRASLEY
                     EPIP"
BASE COUNT          423 a          545 c          542 g          449 t
ORIGIN      
        1 cgagaaaacc ggcagcgatt cgagtcaccc tcacccccgg cccctctccc cccgaacggg
       61 cgaggggaga ttatgggata ggctcgtact ccaatgaagc ccgaacgata taggtttgtg
      121 ccttggccga gaacgtgcgg gcaccatcct ctgtgacccc aacctctaca ggcacgccac
      181 gctcgtcaaa aatcgtgact tttgtaaact tctctggaac acggagtcgt atcacttggg
      241 gaacctcagg attgagctga gcttctagag attgtttgtc ccaccggata gagacgccta
      301 cctttccccg agcacgcagt ccggcgatac tgccagtttc ccaggctgcc ggcaaagcgg
      361 gcaagagatt aatctctccg ccgtgacttt gcaaaagcat ttccgcaatg cctgccgtgc
      421 caccaaagtt tccgtcaatt tggaagggag gacaattgtc aagcatattc ggcaaggtac
      481 tttgcgaaat caactcgctc aacagtttgt aagcccggtc gccatcggcc aatcgtgccc
      541 acaggctgat tttccacgcc ttgctccaac ctgtgccgcc gtcccctcga aattcgagtg
      601 atcgtcgcgc tgcctggcac aattcgggcg agccacgcag cgtgatttct tcaccaggga
      661 aaagacccca gagatgcgat aaatgccgat gcttgttgtt tgggtcatct ttgtcttcca
      721 gccattcttg gagttggcca aattcgccga tctggttggg ggcaatgcgc gcatgcatct
      781 ccataagccg tttacggaag tcagcgtcca cgccgaggat ttcggccgcc ttggccgtcg
      841 tggcaaaaag atagcggatg atctgatggt ccatggtggg gcccatgacg agtccgccct
      901 gttcgggcga gttggacggt ccagagatga gccaccgttg cggtgaacga gggtcctcaa
      961 ccaaatagtg catgaaaaat tgacacgctc ctttcatgag gggatatcca cgcttggcca
     1021 gaaagtcacg gtcttgggtg tactcgtagt gccaccaaat atgttggcat aaccaggcac
     1081 ctccggtcgg ccaaatgccg tgattcgccg cattgatggg tgccgttccc cgccagagat
     1141 caaaattgtg gtgaagaacc caccccgggg cgtcatagtg caccttggcg gtgcgggcac
     1201 cagagatggc tatttcctct atcgcatcaa aaagcggttc cgcacattcc gacaggttac
     1261 agacctcagc cggccagtaa ttcatttcga cattgatgtt tgccgtatat ttcgattccc
     1321 acggaggtga caggctttcg ttccaaatcc cttgcaagtt cgccggttgg gtgccgggac
     1381 ggctgctcga gatcagcaaa taccgtccaa attgaaaata gagaacgcaa agttctgggt
     1441 cgtcttgttt gtcgaaattg cggatccgta ggtttgtggg ttgttgcgct gcctccgtgc
     1501 ttggcaaggc aaactgcaca cgatcgaaga gacttcggta gtcagccata tgtcgagcca
     1561 gcagctcttc gtacgacctt gttctggcct tctcgaggat ttcggcgcaa cgcgcagagg
     1621 gatcagcact aacgtcctca aaatcccggt aattcgtggc tgccgtcagc aggatcgtca
     1681 ccgcgtgggc cccggagact tgaagggctt catcatgcgt gacaagagaa cctccttccg
     1741 gttgcacaag ggcgcgcacc tcaaagcgga tggcacttgg cattgtttcc tttgtccgct
     1801 tatcgatgta gtctgacacc tttccgcgga gaatgatcgc atttgaggcc gtttggatcg
     1861 cgtgctccac atggggtgtc ttgagagagg ccttgaggcc gacggaaccg ggcttatcgg
     1921 ccgtgacacg cacaacgatg acttggtcgg gatagctgg
//
LOCUS       DAOPTR010000814         1957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_45663_length_1957_cov_2.297056, whole genome shotgun sequence.
ACCESSION   DAOPTR010000814 DAOPTR010000000
VERSION     DAOPTR010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45663_length_1957_cov_2.297056"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PKI05_13435"
     CDS             <1..310
                     /locus_tag="PKI05_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53251.1"
                     /translation="RLLYANVDVLILDEPTRGIDVGSKAQIYRLIAEAAEGSPEYGTR
                     PRAVLMVSSYLPELLGLCDRIAVMYRGQLVACAPVGDWTEHKLMLAATGQTSELAEHQ
                     "
     gene            373..1392
                     /locus_tag="PKI05_13440"
     CDS             373..1392
                     /locus_tag="PKI05_13440"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA53252.1"
                     /translation="MSTLGRFTALIVVFLFFAILVEGGKFYSPRNLENILRQSAVYAT
                     ASLGMTMVIIAAGIDLSIGSIIALSLVTVAWILDYHYLVPTADGGMETVYLIRVYPVV
                     LPWIALLAGIGVGTLAGFVNGVTIVRLRLVPFIVTLGTMGIFRGLAKGLAEEKDIYPH
                     EDTWLTQLMDPTLTSQDVHWRMWILPPGVWILIAAAIFAAVLLRYTRLGRYMYAIGSN
                     EETARLCGVPVGRVKVVTYTLAGFFGGLAGLMQFSFIGGIGQPTSAVAYELFVIAAVV
                     IGGGSLMGGQGSILGSVIGALIITILYMGGQQMGWPKWVQEAVIGVIIIAAVVIDRLR
                     QQKTG"
BASE COUNT          402 a          495 c          565 g          495 t
ORIGIN      
        1 gagactactc tacgcaaatg tggatgtctt gattctggac gagccaacac gaggcatcga
       61 tgtagggtcc aaggcccaaa tctatcgttt aattgctgag gctgcggagg gatcaccgga
      121 atacggtaca cgtccgaggg cggtcctaat ggtatccagt tacttaccgg agcttctcgg
      181 attgtgcgat cggattgccg tcatgtatcg gggacaactt gtggcctgtg cccccgtagg
      241 tgattggaca gaacataaac tgatgcttgc agcaactggt caaacttccg aacttgccga
      301 acaccaatga gcgagacacc gaaaaagcgt gcgacgagtc tgcggcaaag tgaaaggtgg
      361 aatgcttcct ttttgagcac tctgggccga tttacggcac ttatagtggt cttcctattt
      421 tttgccattt tggtggaagg gggaaaattt tattcgcccc gcaacctcga aaacatcctt
      481 cggcagagtg ccgtctatgc cacggcctcc cttggtatga cgatggtgat cattgctgcc
      541 gggatcgatc tttctattgg atcgatcatc gctcttagtc tcgtgactgt tgcatggatt
      601 ctggattacc actatttggt cccgacagcc gatgggggaa tggagaccgt ctatctcatt
      661 cgcgtctatc ctgtcgtttt gccgtggatt gccctgctcg ccggtattgg cgtagggacc
      721 ttggcgggat ttgtgaacgg cgtgaccatt gttaggctga ggctcgttcc atttattgtg
      781 accctgggca caatgggcat ctttcgcggt ctggcaaagg ggcttgccga ggagaaagac
      841 atctatcctc atgaagacac ctggcttacg cagcttatgg atccgacgct cacgtcccag
      901 gatgttcact ggcggatgtg gattctccct ccgggggtgt ggatccttat tgccgcagcg
      961 atatttgcgg cggtcctcct tcggtacacg cgattggggc gatacatgta cgccattggc
     1021 tcaaacgagg aaacggcacg gctttgcggg gtccccgtcg gccgagtcaa ggtggtgacc
     1081 tacacgctgg cgggtttttt tggtgggctg gcagggctta tgcagttttc cttcatcggc
     1141 ggcatcggcc agccaacttc ggctgtggcc tacgagcttt tcgtcatcgc tgcggttgtc
     1201 attggcggtg ggagcctcat gggcggtcag ggctccatct tgggaagcgt catcggtgcc
     1261 ctcatcatca caattcttta tatgggcggc caacagatgg gttggcccaa gtgggtgcaa
     1321 gaagcggtca tcggtgtgat catcattgcg gctgtcgtga tcgacaggct cagacagcaa
     1381 aaaacgggat aggtgctccc caccagtaat catttggttt ccaccgcgat aagatgccca
     1441 tagctcggat ggctcttctg ggatgaatcg caggccggat tgtgtttggc cggatatact
     1501 cttcgatctg ccgaccggca taacttatac cgtcggaaaa attgtcaggc cgtaccccta
     1561 ttcgggggag aaccatcctt ttccgcgtcc gttaagtcct caagttttcc cctgaccgca
     1621 aaacttgcaa cctatagttt gagtgacgag ccgcggtcac gacttatcgc tcgtgggtga
     1681 gacgaaagag actcgtctgg tcggcttatc gtcgcaggtg attgatactc ttgtcccgtg
     1741 tatggcgcaa cctaattgcc tgcacagcgc gatgcgcaaa caaacaccga tgagtagcaa
     1801 ctttggtgag gtattccatg agtcggtttg cacttgtcag gggggatggc tgcgcaagtg
     1861 ggacgggcgg acgtaccact ctgaggacaa aacctcataa ccatccttcc gcccctggac
     1921 gttgcgtgcg aaaaggctgt tcgcggaaat cgaataa
//
LOCUS       DAOPTR010000815         1954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_45765_length_1954_cov_2.506582, whole genome shotgun sequence.
ACCESSION   DAOPTR010000815 DAOPTR010000000
VERSION     DAOPTR010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45765_length_1954_cov_2.506582"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PKI05_13445"
     CDS             <1..228
                     /locus_tag="PKI05_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53253.1"
                     /translation="FLPGHVFQIRDGSANLGNSPLGNSPPMLAAAKLSFLQGIVWDKE
                     HPFLTRTDRTGSLVKRLCPAWRRGRILDPER"
     gene            complement(568..729)
                     /locus_tag="PKI05_13450"
     CDS             complement(568..729)
                     /locus_tag="PKI05_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53254.1"
                     /translation="MARRRYFYPRGGGGEIIVSERFKQICERHAVKNACFVPAEEYSW
                     DYYPHETKD"
     gene            complement(1269..1502)
                     /locus_tag="PKI05_13455"
     CDS             complement(1269..1502)
                     /locus_tag="PKI05_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53255.1"
                     /translation="MKSFSTEAILSLRAGRLRPSAETWHVGGSYQNATYDDYNYEYRW
                     TKTWQGQGAGAASSLATNHVTGLEDGAATGWWS"
     gene            complement(1489..1923)
                     /locus_tag="PKI05_13460"
     CDS             complement(1489..1923)
                     /locus_tag="PKI05_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53256.1"
                     /translation="MDDSIYLTALERAVMEMLLAGDHPVLAVLRAQFDRAKVAQSEFS
                     GVGFFTHFEVPPDVPRLRGRKSFELGDVHADIPGLSVGVGFILFIREGAIDFLEGFTY
                     GDDRWHDKIEQYTLCYEKNIGGTVVPAAERDWESLRKKLEEL"
BASE COUNT          411 a          630 c          499 g          414 t
ORIGIN      
        1 tttttgcctg ggcatgtctt ccagatccgg gacggctccg cgaacctggg caactctccc
       61 ctgggtaact ctccgccgat gctggcggcc gccaagttat ccttcttgca gggcatcgtg
      121 tgggacaagg agcatccgtt cctcacccgc acagacagga cgggtagcct tgtgaagcgg
      181 ctatgccctg cgtggcggcg ggggagaata cttgaccctg aacgctaaag ctaagttggc
      241 cccgcagcca cctccaacag cgcttgttgg cgtccgctcc cacatcacaa gcaatcactc
      301 gtcagctccg caatgactga cctccgcgtt tcctcacgta cttcgcgatc cagggagcgg
      361 ccagattgta acgggcatcg tgtgccgcgt ctcctatggg gtcgattttt ccctcaaaac
      421 gttcccgcaa cgcctcgatc caatcgaact ggggtctctg aggacgtgct ttctcatctc
      481 ctccatggcc cgtacgtaat catcgagctg gcccgccttg ttcatggtcc gcaaaaccgc
      541 gagcgtctca tcgtagatgc gaataggtca gtctttcgtt tcatggggat aataatccca
      601 cgaatactct tccgccggaa caaaacaggc gttctttacc gcgtgccgtt cgcatatctg
      661 cttgaatctc tcagaaacaa tgatctcgcc gccgccaccg cgcggataaa aatatcttct
      721 cctggccaag tctcctcctt gatcacgatg cccttccacc ggcttaccgg cggccccaca
      781 agacacaccg gacaggtggg ccctggctct ctcgcttcgt accccgaggc cgcctcgtct
      841 accaccgcct gactacggca taccaacgcg cgaaagtaag cgggcggtgg ttctttggct
      901 cggctccgag gtcggacctt cgccaattcc accgggtaaa aactgcttag ccctcgcagc
      961 cccttatccg cgaatatctc tctgaaccgc aacgaaacga gaatgtcatt attcggggga
     1021 tatacaaggt cacaataccc ctggcggcaa acccagaagt tcaccttgag cggcggcagc
     1081 aagcccgcat cccgatgaat ttgccacacg cttcgcagcg gggcggctcg ccgattacgg
     1141 catcgtcggt gtgtacggca tcgcaaccat gcaggccgac acccctgtgg agaacgtaaa
     1201 agcgaacaga cggatgcttc atttcggtaa cccctcgggg aaacgtttcc tctgccctac
     1261 ataatggact agctccacca gccggtggcc gcgccgtcct ccaggccggt cacgtggttc
     1321 gtggccaagc tactcgcggc gccggccccc tggccctgcc aggttttggt ccaccggtac
     1381 tcgtaattgt agtcgtcata ggtggcgttc tgatagctgc caccgacgtg ccaggtctcc
     1441 gcagatggcc gcaatcgccc agccctgagg gacaagatgg cctccgtact aaagctcttc
     1501 aagcttcttc ctaagcgact cccaatcgcg ctcggcggct ggcacgacgg ttccaccaat
     1561 gttcttctcg taacacagcg tatattgttc gattttgtca tgccaacggt cgtcgccgta
     1621 cgtaaatccc tccaggaaat cgatcgcgcc ttccctaatg aacagaatga aaccaacccc
     1681 cacactgagt cccgggatgt cagcgtgaac atcacccagt tcgaaggatt tgcgtccgcg
     1741 aagcctcggg acatcagggg gtacctcaaa atgagtgaag aaacccactc cgctgaattc
     1801 gctttgtgcc accttcgcgc gatcgaactg cgcgcggaga actgccaaaa cagggtgatc
     1861 gcctgcaagg agcatttcca ttaccgctcg ctctagtgca gtaagataaa tcgagtcgtc
     1921 catctaaggc ggctcacgaa gtacgacacg gaat
//
LOCUS       DAOPTR010000816         1953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_45786_length_1953_cov_3.260274, whole genome shotgun sequence.
ACCESSION   DAOPTR010000816 DAOPTR010000000
VERSION     DAOPTR010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45786_length_1953_cov_3.260274"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(869..>1953)
                     /locus_tag="PKI05_13465"
     CDS             complement(869..>1953)
                     /locus_tag="PKI05_13465"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOA53257.1"
                     /translation="PGSEHFLPDHPAAARNTAGLPVRIAIAPPKDPQFAHLSWNKALR
                     TAKGTIVLTYIAGAFHGAHGGGCPAVSYSTDGGQTFSEPNVLRQFGPGRDYSCSGNLA
                     LGVAPDGAVVLLAMAYTGNESNHIFGWRSEDEGVSWTPVDTGTLGPNKTGSVFGNILA
                     VPGRGLLVFGHYRLGAQPYTSGIWMAASADSGRTWGLPRRISEVPAVEPLVLQVGSRL
                     IGFFRGSDALRGRQFVAISDDLCDTWKTELSVLGPEDPNTSGLAAPFAVENPGRPGEI
                     LVLTTERALAGSTPGRIWLWRGDAQHLDWRRDRVLLEFPRIPGDPHTDFGYPWLVPLE
                     NGRWLMFYYHGRKRGHCPIWVTEVTI"
BASE COUNT          423 a          605 c          551 g          374 t
ORIGIN      
        1 ttcgagattc tccgcaaacc attctccgct cggcagcttt cctcggtcgt gcgaagcgct
       61 ttagagcaat cttatcgctt tcttcccaaa aggatttgac ggccgccctc ttgcccacac
      121 cggatcgcgg cagcaccacc cccgcatggg gcgagaattc gcatcctaca ccatcaccct
      181 gcaataacgg acgagcgatg ggggattcga ctggtgtgac gtaaattatt atcaaaaaca
      241 acttgtgtca attcgaccca tccaattggc acaaaatccg gaacacttga tgataactcg
      301 ccgatgagcg atggacgtga cgaccgtggg atattcacca ttgacaaccc cggcggatgg
      361 gaccatgaag ccgcacttgc gacataggcg cggttaccag aattgtggcc acggttacct
      421 atagaggttc accgctagat tgtggccatc gcagagggat ctgctgccac cgagacagga
      481 atgcgtgaag caagacacct caccgcaacc gttgttgcct catcaaacac gcgccgcctt
      541 gggcaccgtt ttggcgaagc ggcatccggt tgtttcttgt tgcgcattct aggatggtag
      601 ggcctgtacc gccgtgggcg ccagtcgtgt ttgcccatgg gaatcacgtt gaacaggacc
      661 gccccgagga ttgttccaat ccgggcaacc acagggggtt gcccgtacac accccatcaa
      721 aacagtaggg gcaggccccc gtgcctgccc ggaccccgta cgtactcgac gcccccgtgc
      781 ctgccggaga ccgcggacgt cccgaattgc acgtaggacg cgggggaccg cgtccctccc
      841 attcccatcg gcggacacga agttagattc agattgtcac ttctgtaacc caaatcgggc
      901 agtggcctct tttgcggccg tgatagtaaa acatcagcca tcggccgttt tctagcggaa
      961 cgagccaagg atacccgaaa tcggtgtggg ggtcgccggg gatgcgcgga aattcgagca
     1021 agacgcgatc gcgtcgccag tcaaggtgtt gagcgtcgcc ccgccatagc cagatgcgtc
     1081 ccggcgtgct gccggccagt gcccgctcag tcgtcagcac cagaatttcc ccaggccgac
     1141 caggattttc cacagcaaat ggggcggcca atccagatgt gttggggtcc tcgggcccca
     1201 agaccgacag ttcggttttc caggtgtcgc acaagtcatc ggagatcgcc acaaactgcc
     1261 ggccacgaag cgcatcggat ccgcggaaaa acccaatcaa tcgtgaacca acttgaagca
     1321 ccagcggctc aactgctggg acctcgctga tccgacgggg cagaccccac gtgcgaccac
     1381 tgtccgccga tgctgccatc cagataccgc tggtataggg ctgggcaccg aggcgataat
     1441 gtccgaacac caagagaccg cgtccgggaa ccgcgagaat gttgccgaac acggaacctg
     1501 tcttgtttgg accgagagtc cctgtgtcca cgggcgtcca acttaccccc tcgtcttcgc
     1561 ttcgccagcc aaaaatatgg ttgctttcgt taccggtgta ggccatggcc aacaacacca
     1621 ccgcgccatc tggagcgaca cccaaagcca agttaccgct gcacgagtag tctcggccag
     1681 ggccgaactg gcgcaacacg ttcggttctg aaaaggtttg cccgccatcg gtcgagtacg
     1741 acacagccgg acagccaccc ccgtgcgcgc cgtgaaatgc gccggcgatg taagtcagca
     1801 ctatcgtacc cttggctgtg cgtagcgctt tgttccacga aaggtgggcg aattgcggat
     1861 cctttggtgg tgcgatggcg atgcgaacag gaagccctgc cgtatttcgc gccgccgccg
     1921 gatgatccgg caagaaatgc tctgatccgg gtg
//
LOCUS       DAOPTR010000817         1951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_45861_length_1951_cov_2.540612, whole genome shotgun sequence.
ACCESSION   DAOPTR010000817 DAOPTR010000000
VERSION     DAOPTR010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45861_length_1951_cov_2.540612"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PKI05_13470"
     CDS             <1..574
                     /locus_tag="PKI05_13470"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53258.1"
                     /translation="AAHEKGYETSANLMAVSHATEVEIEQCLGLISQTPAEVVVVVDS
                     FGALYTEQIERLVKIYLSYVKDKGKTVGIHAHNNQQLAFANTIEAIIHGANRVDASMG
                     GLGRGAGNCPMELLIGFLRNPKFRIRPVLQFLQDYIEPLRNEVEWGPYVQYNITGQLN
                     LHPRSAIEARKNPEDRNKYVDFFDRVSTEA"
     gene            complement(827..1270)
                     /gene="crcB"
                     /locus_tag="PKI05_13475"
     CDS             complement(827..1270)
                     /gene="crcB"
                     /locus_tag="PKI05_13475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HOA53259.1"
                     /translation="MERFRLLGTLALVTLLSSDVVLIGLGGGLGSVARYLVGKLIHEG
                     SGENVFPIGTLVVNVLGCFFLGMLIGVFLEQGSHRVTGWLNFLGTGFCGGFTTFSALQ
                     WETVRLIHDGRWVVAAVNVGLSIFAGLVGIVVGYLIGRWVSFYVD"
     regulatory      complement(1399..1470)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            1758..1832
                     /locus_tag="PKI05_13480"
     tRNA            1758..1832
                     /locus_tag="PKI05_13480"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1793..1795,aa:Pro,seq:cgg)
BASE COUNT          487 a          534 c          512 g          418 t
ORIGIN      
        1 ggctgcccac gaaaaaggtt atgagacgtc ggctaatttg atggcggtct cacacgccac
       61 tgaggtcgaa atcgagcaat gcctcgggct catatcgcag acgcctgctg aagtcgtcgt
      121 cgtagtcgac agtttcggcg cactttatac cgagcaaatt gagcgccttg ttaagattta
      181 tttgagttat gtcaaggaca aaggcaaaac cgtgggaatc cacgcccata acaatcagca
      241 actggctttt gccaacacca tcgaggcgat cattcatgga gcgaaccgcg tcgatgcatc
      301 catgggtggt ttggggcggg gagcgggcaa ctgtcccatg gagctcctga tcggcttcct
      361 ccgcaatcct aagttccgga ttcgccccgt gttgcaattc ttgcaggatt acatcgagcc
      421 gctgcgcaac gaggtggaat gggggcctta tgtccagtac aacatcacgg gccaacttaa
      481 cctgcatccg cgatcagcga ttgaagcccg aaaaaacccg gaagaccgaa acaagtacgt
      541 cgatttcttc gatcgggtaa gcactgaggc gtaagacgct atttgtccgg tcagcggtga
      601 tagctttctg actaaagaag gaagtaggga cggaccattg tacgtcattc tttttatcat
      661 cggcacagga atcatcaact tcgtagccgg gttcctcctc gcaatggcga tcggctatgg
      721 tccagcgagt tggaacgagg tgcggaaatt tcttgctggc tgagcgtgca atcaggtgtc
      781 ttgaaattcg accgacgatc gtgtcaagaa tcgctcgcaa tctcggctag tccacataaa
      841 aagataccca ccgcccgatc aagtaaccga cgacaatacc gaccaagccg gcaaaaatac
      901 tgaggccaac gttgacggcg gccacaaccc agcggccgtc gtggataagc cggacggttt
      961 cccattggag agccgaaaac gttgtaaatc ctccgcagaa tccggtgccc aggaagttca
     1021 gccatccggt cacccgatga ctcccctgtt cgagaaaaac cccaataagc atcccgagga
     1081 aaaagcatcc caaaacgttg acaaccagcg tgcctatcgg aaagacattt tctccacttc
     1141 cctcgtggat tagctttccc acgagatagc gggcgaccga accaagtcca cctcccagac
     1201 cgatcaggac gacgtcggaa gatagcaacg tgaccaaagc aagggtgcca agcaaacgaa
     1261 aacgctccat gttgcgaacc cagatccctt tacagtgtga gaccaaccgc tgcacgagga
     1321 ttctcggtgg ccggccgtga gatccccgcc gagttaacca cttcggtagg gcaaggccaa
     1381 aagaaaaccc ctaccgaaaa ccaggtaggg gtcatcagta gctagcatgc gaagctacaa
     1441 ttagtggcga accccatcgc cattatactt tctttaaata tagcttatcc cgtccgccag
     1501 ggaagagggg ctggcccaaa aaacttccgc gggcgtggcg gtcgccaagc ctggtaacct
     1561 ttgacgatta ttccgttaca tcggcttagc aatccctgag acgtaatatt ttcggctggc
     1621 tctccttttg cttgaaaggc ttaatggctc ccgattgacg gcgtccaaat caaccgatta
     1681 cactttaagg acgacggata tgggtcagcg agggcgtttt ttgccgcccg gttggccttt
     1741 tctaatgagt taacgcccgg ggcgtagctc agcctggcta gagcgcttgg ttcgggacca
     1801 agaggtcgct ggttcaaatc cagtcgcccc gattagatga gcgagagatg tgactcatcc
     1861 tacgtttcgc ttttcgtcgc tgaacatcgg ccgacacgcg accgtaacta gccgccggaa
     1921 gggggattta cctggtcctt ttgtggtact c
//
LOCUS       DAOPTR010000818         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_45905_length_1949_cov_3.412355, whole genome shotgun sequence.
ACCESSION   DAOPTR010000818 DAOPTR010000000
VERSION     DAOPTR010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45905_length_1949_cov_3.412355"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /gene="dapF"
                     /locus_tag="PKI05_13485"
     CDS             complement(<1..697)
                     /gene="dapF"
                     /locus_tag="PKI05_13485"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOA53260.1"
                     /translation="MRFTKMHGIGNDYIFVDCFHETLAEAPETLAAKVSERHFGIGGD
                     GLILILPSNVADARMRIFNADGSEAEMCGNGVRCVAKYVYERGIAKKNPLTIETGRGV
                     LTLHLEVKDDRVQSVTVDMGEPILDPARIPVRLNGTVIEHPINMDGVDLAITCVSMGN
                     PHCVIFRPEIDDLLVTQIGPKLERHPLFPNRTNVEFVQVLSPSEVRMRVWERGSGETL
                     ACGTGACAVCVAGV"
     gene            complement(862..1182)
                     /locus_tag="PKI05_13490"
     CDS             complement(862..1182)
                     /locus_tag="PKI05_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53261.1"
                     /translation="MSIGPLGHVPASAAGSPLAQIKGDANQAKHTSDVQQRRVALDQK
                     AESAAGIGETDGEDHETEERDADGRRVLEIPAREKKDEETSDQAQNPKDATGQLGRLL
                     DLSG"
     gene            complement(1290..>1949)
                     /locus_tag="PKI05_13495"
     CDS             complement(1290..>1949)
                     /locus_tag="PKI05_13495"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOA53262.1"
                     /translation="ELFGGQIMGGGWIRMSPELAYDLAGSLVTADFQQILRQYHHNDG
                     VNARGKVDATIRIRGKGDDIQGLSGYGNFQFREADIYELPIMLALLKLFDLRRPDATT
                     AFTESTGSFRIAGRHVYLDSITFRGEMFHLTGQGEIDFDHNVRLVLRVMLGKKEMPIP
                     VIKEIFRGASEQIVLVHVGGTIYEPIVRRESFPGVNQAIQRLQNTVESLGQREASANW
                     W"
BASE COUNT          411 a          572 c          492 g          474 t
ORIGIN      
        1 ggacccctgc gacacaaacc gcgcacgccc cggtgccgca ggccagcgtc tctccagagc
       61 cgcgctccca tacccgcatc cgaacctcac tcggagaaag gacttgaaca aattccacat
      121 tggtgcgatt gggaaaaagg ggatgccttt cgagtttggg tccaatctga gtcaccagaa
      181 gatcatcgat ctccgggcgg aaaatgacac aatggggatt acccatcgaa acacaggtga
      241 tggcaaggtc cacaccatcc atgttgatgg gatgctcgat gaccgtgccg ttaagccgca
      301 ccgggatgcg ggctggatcg aggataggtt ctcccatatc gacggtcaca ctttgtactc
      361 gatcatcttt gacttccaag tggagggtga gcacaccccg acccgtttcg atcgtcagcg
      421 gatttttttt ggcgatccca cgttcataga cgtatttggc cacgcaacgg accccgttgc
      481 cacacatttc ggcctcagag ccatcggcat taaagattcg cattcgggcg tcggcgacat
      541 ttgaggggag aatgagtatc agtccatcgc ctccgattcc aaagtgtctt tcagacactt
      601 tggcggcaag agtctccggc gcctcagcaa gtgtctcatg aaagcagtcc acaaaaatgt
      661 agtcgttgcc aataccgtgc atcttcgtga atcgcatagg tagaactaac ctttcgtatg
      721 tagcaatgtc atccgctgct tagtgtacac ctttatcgtg atgcctcctt gttttatctg
      781 acaaggggct gcaccccccg ccatttgtga ttgttccttc atggcgagat cagactctgg
      841 ttcggtctca gaacctgggg ttcacccgct cagatcgagc aaacgcccca attggcccgt
      901 tgcatctttg gggttttggg cctgatctga agtctcctcg tctttctttt cgcgtgccgg
      961 gatttccagc acacggcgac catcggcgtc gcgttcttct gtttcgtggt cctcgccgtc
     1021 tgtctcaccg atccccgccg cggactcggc cttttgatca agcgcgacgc gccgttgttg
     1081 cacgtccgaa gtgtgtttgg cctgattggc atcgcccttg atttgggcca gagggcttcc
     1141 ggcggcagac gctggcacat gtcctaacgg cccaatgctc attgcggtat cctccgctgt
     1201 ctgctgaaac actgcggcga aaggatccca cttagctttt aaaacccttg atgtcgtttc
     1261 ctctcccaac cgcgtaccct aacgaatctc taccaccaat ttgcactagc ctcacgctgt
     1321 cccaatgatt ctacggtatt ctgaagtcgt tgaattgcct gatttacacc ggggaacgat
     1381 tcccgccgga caatcggctc gtagatggtc ccgccgacgt gcaccaggac gatttgttca
     1441 ctagccccac ggaagatctc cttaatgacc ggtataggca tttccttttt ccccaacatg
     1501 acccgcaaga caagtcgcac gttgtggtcg aaatctattt cgccttgtcc cgttaggtga
     1561 aacatctccc cgcgaaaagt gattgagtct aagtaaacgt gccgccctgc gatgcgaaag
     1621 gatccagtgc tttccgtgaa ggctgtggta gcgtcgggtc gccgaaggtc aaacaacttc
     1681 agtagggcca gcataatagg aagttcatag atatcggcct cgcggaattg gaaatttcca
     1741 tagccgctca gtccctggat gtcgtcgccc ttgccgcgaa tacggatcgt ggcatccact
     1801 tttccccgtg cgttaacacc gtcgttgtgg tggtactgcc gcaaaatctg ctgaaagtct
     1861 gccgtgacca gcgatcccgc caggtcgtag gcaagctcgg gcgacattcg gatccaaccc
     1921 cctcccataa tttggccgcc aaaaagctc
//
LOCUS       DAOPTR010000819         1948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_45936_length_1948_cov_3.588484, whole genome shotgun sequence.
ACCESSION   DAOPTR010000819 DAOPTR010000000
VERSION     DAOPTR010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45936_length_1948_cov_3.588484"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PKI05_13500"
     CDS             complement(<1..747)
                     /locus_tag="PKI05_13500"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOA53263.1"
                     /translation="MTRCAAILGGLLTVCLLASGTWAESTSSAQGGKIRVLLTYGGHG
                     FEEKPFFEMFDNMEGIEYKAIKLPDQADMLNPKAADEFDVIVMYDMVPAISEKQQEAF
                     VALLKKGIGVVSLHHNMGAHRNWDEFRKIIGGKFILEDCVIDGQEYKKSGWAHGQDYK
                     VIIADPNHPITRGLKDFIIHDETYKDYYTAPNVHVILKTDCPTNDPEIGWVTKYGESR
                     VFYLMLGHDSLAWKNPNYPVILKRGIEWAAG"
     gene            complement(923..1558)
                     /locus_tag="PKI05_13505"
     CDS             complement(923..1558)
                     /locus_tag="PKI05_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53264.1"
                     /translation="MYQVLCCGMFILAATGLSVYGQAEEASPIFADSFREKLAEGWAW
                     LRELPGGWRIREGGLEIRALPGDANTVKNALLRPAPDRTAGPFAVEVTVSNVTPPTVQ
                     YEQVGITWYVDEKPVFKLVKELIDGKIYIIPGRKPVENVPIRLRLVIDGDRWTAQYRP
                     AGEKEFLVAAEGTLPPPANDHVSLQCYHGPENAEHWMRFEDFRILRLPSKR"
     gene            complement(1618..>1948)
                     /locus_tag="PKI05_13510"
     CDS             complement(1618..>1948)
                     /locus_tag="PKI05_13510"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOA53265.1"
                     /translation="ALNELAQDAHVGFVIDESRITVMPPVASACEMLGLDPLYVANEG
                     RFVAIVSPEAADDLLEVMRGQGEAVGLQIIGEVRAQPAGVVELRTAYGSSRILDWLSG
                     EQMPRIC"
BASE COUNT          440 a          559 c          477 g          472 t
ORIGIN      
        1 acctgccgcc cattcgatcc cccgtttgag gatcacggga taattggggt ttttccaggc
       61 gaggctatca tgccccaaca tgaggtagaa tacccggctt tctccatatt tggtgaccca
      121 accgatttca gggtcattcg tcgggcaatc cgttttcaag atgacgtgca cattgggggc
      181 cgtgtaataa tccttgtagg tttcatcgtg aatgatgaaa tctttcaatc cccgggtgat
      241 tggatggtta ggatcagcaa tgatcacctt gtaatcctgg ccgtgagccc agcctgattt
      301 cttgtactct tgtccatcaa tcacacaatc ttcgaggatg aacttgccac cgatgatttt
      361 tcggaattcg tcccaattac gatgggcacc catgttgtgg tgtaacgata ccacaccgat
      421 gccctttttg agcaaggcaa cgaacgcctc ttgttgcttt tccgaaatag cgggaaccat
      481 atcgtacatg acgataacat caaactcgtc tgctgccttg ggattgagca tatcggcttg
      541 atccggcaat ttgatggctt tgtactcgat cccctccata ttgtcgaaca tctcgaagaa
      601 tggcttttcc tcgaagccgt ggccgccata tgtcaaaagg acacgaatct ttcccccttg
      661 cgcggagctc gttgattcgg cccaagtccc actggccagc agacataccg tcaacaaacc
      721 acccaagata gccgcgcacc gtgtcatcgt tcgacctcct caaggacaaa gcccgtcaac
      781 acaggattcc tacaatactc tgccaaaaca cgtaggtatt taggtgtgat ttttggtgat
      841 ttaaaaatgg ttaaacttgc gtgacgacac cgaagatcat tcctcagtgc caacttcctc
      901 ccaccccgtt aagttctttc gatcaacgtt tgctaggcaa ccggaggatg cgaaagtctt
      961 cgaatcgcat ccagtgttct gcattctcag gaccatgata acattgcaga ctgacatggt
     1021 cattggccgg cggtggtaag gttccctcgg ccgcaaccaa gaactccttt tcaccggcag
     1081 gacgatattg cgccgtccag cggtctccgt caattacgag ccgcaagcgg atgggcacgt
     1141 tttcgacggg ttttcgcccc ggaatgatgt atatcttacc atcaatgagt tccttgacca
     1201 atttgaaaac gggcttctca tccacgtacc acgtaatgcc cacctgttcg tattgcactg
     1261 ttggtggcgt gacgtttgat accgtgacct ccacggcaaa tggtccagcc gtccgatcgg
     1321 gcgcaggccg cagaagggcg ttcttgaccg tattggcgtc gccgggcaag gcgcgtatct
     1381 caaggccacc ttcgcggatc ctccagccac cgggcagttc tcgcagccag gcccatccct
     1441 cggccagttt ttctcggaat gaatcggcaa aaattggaga tgcctcctca gcctgtccgt
     1501 aaaccgacaa accggtggca gcgaggatga acattccgca gcataacact tggtacatgg
     1561 tgtatcccct cattgatgtc ctacatatgg gcaaggaaac ggtcacgctg gcgtaagcta
     1621 acaaatccgc ggcatttgct ctccggaaag ccaatcgaga attcgggaac tgccgtaggc
     1681 tgttctaagc tcgacaactc cggccggttg tgcccgcacc tcgccaatga tctgcagtcc
     1741 aacggcctcg ccttgtcccc gcatcacttc caagagatca tccgctgctt cggggctgac
     1801 gatggctacg aaccggcctt cgttggccac gtacaagggg tccagtccca gcatttcgca
     1861 agcagatgcg acgggcggca tcaccgtgat gcgcgattcg tcgatgacaa accccacatg
     1921 ggcgtcctgt gccaactcgt tgagcgcg
//
LOCUS       DAOPTR010000820         1948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_45949_length_1948_cov_2.797676, whole genome shotgun sequence.
ACCESSION   DAOPTR010000820 DAOPTR010000000
VERSION     DAOPTR010000820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45949_length_1948_cov_2.797676"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1486)
                     /gene="aspS"
                     /locus_tag="PKI05_13515"
     CDS             complement(<1..1486)
                     /gene="aspS"
                     /locus_tag="PKI05_13515"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOA53266.1"
                     /translation="MLRTHTCGELRTSHIGQHVTLCGWVDTYRDHKGVVFVDLRDRYG
                     KTQIVFSSDNGQELHQIGRSLRPEYVIQVTGLVERRPEGTENPKLATGEIEVRAEQCC
                     ILNRANVHPFLPSTPDLPGEEVRLRYRYIDLRRPQMQQILLLRHRMIKVMRDYFDEHG
                     FIEVETPMLGRSTPEGARDYLVPSRIQKGTFYALPQSPQLYKQILMVAGYDRYMQIAR
                     CFRDEDLRADRQPEFTQLDLEMSFVQADDVMGIVEGLMARLFREILGQELTLPLPRMT
                     YDEAMERFGHDAPDLRFGMELTDITAIAAECDFRVFREAAENSKRVRGITVPGGANRY
                     SRKQIDELTEWVKQDFGAKGLVWFRVEPEGTLNSTVAKNFTASQLEAIRAKMGAQPTD
                     LLLFVADDFEITCKALYGLRKRFGAELKLYDPRAMHLSWVVEFPMFAIDEEEGGWTSM
                     HHPFTAPRPQDRELLKTDPGKCRAQAYDLVLNGTEVGGGTIRIHD"
BASE COUNT          457 a          540 c          504 g          447 t
ORIGIN      
        1 agtcatgaat acggatggtt cccccgccga cctcggtgcc attgagcacc aggtcgtacg
       61 cctgggcgcg acactttccc ggatctgttt tcaaaagttc gcggtcttgg gggcgcgggg
      121 ctgtaaacgg gtggtgcatg gaggtccagc cgccttcttc ttcgtcgatg gcaaacatgg
      181 gaaactcaac gacccaggat aggtgcatcg cacgtggatc gtacaacttc aattctgcac
      241 caaaccgttt tcgcaaaccg tacaacgcct tacaggtaat ttcaaaatcg tcggcgacga
      301 agagcaacaa gtcggtgggt tgtgcaccca tcttggcccg aattgcctca agctgtgatg
      361 ccgtgaaatt cttggccacc gtggagttaa gcgtgccttc cggttcgacg cggaaccaca
      421 cgagtccctt tgccccgaag tcctgcttaa cccattcggt caactcgtcg atttgcttgc
      481 gggaataacg attggcgcca ccgggcactg ttattccccg cacacgttta ctattttctg
      541 ccgcttctcg aaagacccta aaatcgcact ccgcggcgat agccgtaatg tccgtaagct
      601 ccatgccaaa tcgcaaatcg ggggcgtcgt ggccaaagcg ttccatggcc tcgtcataag
      661 tcattctcgg caggggcaac gtaagttctt ggcccagaat ctcgcggaac aagcgggcca
      721 tcagaccctc gacaataccc atgacatcgt ccgcttggac gaatgacatt tccaaatcga
      781 gctgagtgaa ctccggctgc cgatctgccc gtaggtcttc atcgcgaaaa caccgggcaa
      841 tttgcatata acggtcgtag cccgcgacca ttagaatctg tttgtaaagc tgaggagact
      901 gcggaagtgc gtaaaacgtt cccttttgaa ttcggctagg gacaaggtag tcgcgggctc
      961 cctcgggcgt gcttcggccc aacatgggcg tttcgacttc gataaatcca tgctcatcaa
     1021 aataatctcg catcaccttg atcatccgat gccgcaaaag caaaatttgt tgcatctggg
     1081 gccggcggag atcaatgtat cgataccgca ggcgcacctc ttccccaggc aaatccggag
     1141 tcgacggcaa gaaaggatga acgttcgccc ggttgaggat acaacactgt tctgcccgaa
     1201 cctcgatttc tcccgtggcc aatttagggt tttccgtgcc ctcggggcgg cgttccacga
     1261 gacccgttac ctgaatcaca tactccggac gaagcgagcg gccaatttgg tgcaattctt
     1321 gaccattgtc ggaggaaaag acaatctgcg tctttccgta acggtctcga agatcgacaa
     1381 agactacgcc cttatgatcg cgatatgtat caacccaacc acagagggtc acatgttgcc
     1441 caatatggga ggttcgcaac tcgccacagg tatgcgttcg taacaaagcg ctagctcctt
     1501 tctatacaag aggcaaccga acacgcgact tttggtgatt tgcccaaaac ctctaagtag
     1561 cccacatacg ccagcagcta cagtatcatc tttgcgtttg ggcctagtcg cgcactcact
     1621 gggtgcgttt gaaatcgacg caaaacccct attttaggct ctggaggggc gttttcctag
     1681 atgagtagtg tcggggctca ggccatggca ggggtccgtt ctacggctac aaaccctaaa
     1741 acctgcgcgg cggttcagcc acccagtatg tctctggttc agacaagcca gcctgcacta
     1801 taatagtgca caatcagaaa cgtcctaatc tcgcgtagga ttattggaga ttcggagatc
     1861 aatgaacggc cttttgggga agggatgatc ccgctcttct ttggccacct ttggtagaaa
     1921 gacgtgctgg gagacggaga aaattggc
//
LOCUS       DAOPTR010000821         1947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_45969_length_1947_cov_3.618393, whole genome shotgun sequence.
ACCESSION   DAOPTR010000821 DAOPTR010000000
VERSION     DAOPTR010000821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45969_length_1947_cov_3.618393"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PKI05_13520"
     CDS             <1..630
                     /locus_tag="PKI05_13520"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOA53267.1"
                     /translation="AVSRWRIAFSKKTFPVRLLTTKAIIILLIPAILFSHHVYTEDGM
                     IEETLETLGYALLFIAALGRVWAAAYISGKKGRDLVVDGPYSLVRHPLYMFSALGFIG
                     TGLALGSLTVAAFLLVVFFASHYPAMIYEERFLAQKFGQAAITYFGGTARLWPSFHFQ
                     SGPDVQTINIRVFTRAVLDAALIGLIFPATGALEWLHLHKVIPVLFHIY"
     gene            complement(838..1716)
                     /gene="mutM"
                     /locus_tag="PKI05_13525"
     CDS             complement(838..1716)
                     /gene="mutM"
                     /locus_tag="PKI05_13525"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOA53268.1"
                     /translation="MPELPEVETMRRGVRPLVGGTIIEAFCPPSHHHPIKYRPPPETL
                     FPALVGRSIRAVERCGKRLLLRLDNDHALVIEPRMTGRVSLNPPQLPSHTRLVLRVRM
                     TQPRGNRKGAPVEHTMIFRDVRGLGAVHLLDANSCRTELGPQNIGPDALDISWQELRD
                     RLRHRKCAIKVALLDQRAIAGVGNIYASEALHLARIHPTTPCHLISNRRWRTLQQALH
                     LVLEEAIAKQGSTLSDGAYITPSDEAGRYQDEHRVYQRTGERCIQCRRGIIRRIILGQ
                     RSTFYCPVCQPAPRGS"
     gene            complement(1728..>1947)
                     /locus_tag="PKI05_13530"
     CDS             complement(1728..>1947)
                     /locus_tag="PKI05_13530"
                     /inference="COORDINATES: protein motif:HMM:NF021941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53269.1"
                     /translation="SEAERAREIARKQQELTKLESLIASKRRKLENADFVKKAPPHVV
                     EKERESLRQLEDQRAAALAVVAQLQNQA"
BASE COUNT          379 a          526 c          575 g          467 t
ORIGIN      
        1 gctgtatccc gttggagaat agctttctca aaaaagacgt ttcccgtgcg acttttgacg
       61 acgaaggcga tcatcattct gttgataccg gcgatcctct tctctcatca tgtttacacg
      121 gaagatggca tgatcgagga gacactggaa acgctaggat acgcgctcct tttcattgcg
      181 gctctgggaa gagtttgggc tgctgcatat atctccggga aaaaaggccg cgacttggtc
      241 gtggacggcc cttattcgct cgttcgacat cccctgtaca tgttttcggc gcttggcttt
      301 attgggaccg gcctggcttt aggctccttg accgtcgccg cgtttctgtt ggtggttttc
      361 ttcgcatccc attatccggc catgatttac gaagaacggt ttctagccca aaagtttggt
      421 caggccgcca ttacctattt tggtggtacg gctcgcttgt ggccgagttt ccattttcag
      481 tctggccctg atgtgcaaac gatcaatatc agagttttta ctcgggcagt cctggatgct
      541 gcgctgattg ggctaatctt tccggcgaca ggcgcccttg agtggcttca cttgcacaag
      601 gtgatccctg tgctatttca tatttactaa gagcctagcc caaagttccc tctccctccg
      661 ggagagggtt ggggtgaggg caagcgaccc tcggacttca tcgagaaagc cggcagggat
      721 tcgagccacc ctcacgcccg atccgtctgc cgcacaacgg gtgagggaag gttgtgggat
      781 aggctctaag cgagatgtct gcctgtcggt ttgtcatttc cgtactggct cgaaaagcta
      841 acttccgcgc ggcgcaggct gacacaccgg gcaatagaag gtggatcgct ggccaaggat
      901 gatccggcga ataatgccgc gacggcattg gatacatcgc tcgccggtgc gttgatatac
      961 tctatgctcg tcctggtaac gaccggcctc atcactgggg gtgatatatg ccccgtcgga
     1021 caaggtcgaa ccttgcttgg cgatcgcctc ttcgagtacg agatgtaacg cctgttgaag
     1081 cgttcgccat cgacggttgc ttatgaggtg gcaaggggtg gtaggatgaa ttcgcgcgag
     1141 atggagggcc tctgaggcgt agatgtttcc cacacccgcg atggcccttt gatcgagcaa
     1201 cgccaccttg atggcgcact ttcgatgacg cagtcgatcc cgcagctctt gccaagagat
     1261 gtctagggcg tctgggccga tgttctgagg tccgagctct gtccgacacg aattggcgtc
     1321 gagaaggtga acggccccca ggccacgaac gtcacgaaag atcattgtgt gttcgaccgg
     1381 ggcacccttg cgattccccc gaggctgcgt cattctcaca cggaggacga gtcgggtatg
     1441 gctgggaagc tggggcggat tgagcgagac gcgaccggtc atgcgtggct caatgactag
     1501 agcgtgatcg ttgtcaagac gcaggagcaa tcgcttgcca caacgttcga cggccctgat
     1561 cgaccttccc acaagcgcgg gaaacagggt ttcaggcggc ggcctgtatt taatcggatg
     1621 atgatgtgag ggagggcaga acgcctcgat aatcgtacca ccaaccagcg ggcgaacccc
     1681 gcgccgcatg gtttcgactt cgggcagttc aggcatggcg aagcatgtta ggcttgattc
     1741 tgcaattgag cgacgacggc cagggccgct gcccgctggt cctccaactg tcgcaacgac
     1801 tcacgctcct tttctacgac gtggggcggg gcctttttca cgaaatcggc gttttcgagt
     1861 ttgcgacgtt tcgaggcaat caacgactcg agcttcgtca attcctgttg cttgcgagca
     1921 atttcgcggg cccgctctgc ctcggac
//
LOCUS       DAOPTR010000822         1945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_46041_length_1945_cov_4.165079, whole genome shotgun sequence.
ACCESSION   DAOPTR010000822 DAOPTR010000000
VERSION     DAOPTR010000822.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46041_length_1945_cov_4.165079"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..707)
                     /locus_tag="PKI05_13535"
     CDS             complement(51..707)
                     /locus_tag="PKI05_13535"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOA53270.1"
                     /translation="MAYELPALPYAYDALEPHIDARTMEIHHTKHHQAYITNVNKVIA
                     GTEWEKLAVEDLVAAIHRVPEQIRTVVRNHGGGHANHSLFWTVIGPNGGGEPKGKLAQ
                     AIERELGGFEKFRAAFTAAAMGRFGSGWAWLSVDPNKRLVIEDTPNQNSPLMHGHIPI
                     LGIDVWEHAYYLKYQNRRNEYVEAFYHVINWGEVLRRYEAALAQPVPESAKHPLKTDT
                     "
     gene            1472..>1945
                     /locus_tag="PKI05_13540"
     CDS             1472..>1945
                     /locus_tag="PKI05_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53271.1"
                     /translation="MSFRRPSRATRRLVIESLEQRHLLSVTSLPLPGQFLDLGPVDYS
                     QVTLADSSSAGQWFRLTAQNTATLTAICGPSPADPSPLGVTVYALSGNALVELARGNG
                     RVDTPVTAGSTYFVWLGQVQSGEQLFLANLVQNSGTTVTVVGTAGDDTFEFDGTSR"
BASE COUNT          380 a          550 c          530 g          485 t
ORIGIN      
        1 ttaccttaag gtgatcaacg atcacccggt cgtggggatg ggctgtgttt ttaggtatcc
       61 gttttaagcg gatgctttgc ggattcgggg accggttggg cgagggccgc ttcgtaacgt
      121 ctgaggactt caccccaatt gatcacgtgg tagaaggcct cgacgtattc attcctccgg
      181 ttctggtatt tgaggtaata tgcgtgctcc caaacgtcga tgccgaggat cgggatatgc
      241 ccatgcatta gggggctatt ttgattgggg gtatcctcga tcaccaatcg cttgttcgga
      301 tcaaccgaga gccatgccca accgctgcca aagcgcccca tggccgcagc cgtgaatgcc
      361 gcccgaaact tctcaaatcc tcccaactct cgctcaatag cctgggccaa ttttcctttg
      421 ggttccccgc caccattggg cccgatcacg gtccaaaaaa gggagtgatt ggcgtgcccg
      481 ccaccgtgat tccgcaccac agtgcgaatc tgctcaggca cccggtgaat ggccgccacc
      541 aggtcttcta cagccagttt ctcccattct gtgccagcga tcaccttgtt cacgttggtg
      601 atgtaagcct gatggtgctt cgtgtggtga atttccatcg ttcgggcatc aatgtgtggt
      661 tccagtgcgt catacgcgta ggggagagcc ggtaactcgt acgccatgtg tcaactcctc
      721 gtttgtcaat gaaagtctcc gcttgcaacc cgcgatggct gcgtccctcc gaccagcctt
      781 ccttttctcg cggagctgcc tagatcgaca gtcatggcca ccatattggc catttatgtc
      841 ttacttattt gtctaaataa taaccaaccg gtcctgaaag acaaggccac gaatttctgg
      901 gagttgaacc aatacccgac gtggaccgga gctcggggat taaggagcct atcccacatc
      961 ctcccgtcgc ccgttcggcg cgagaggggc cgggggtggg ggtggctcga atcgctgctg
     1021 gttttctcga tcgagccgga ccgtgtcttg ccctcacccc aaccttctcc caaacggaga
     1081 ggcaattttg gatacgctct aagttcgaat gcgcagcttt tgcgcacatc tgcccgagtg
     1141 gcatttcaca aggcttatca acgtggttca gttgcttacc caagtccgtt cgaaacgttg
     1201 ccacttgcac actgactttg gccctccgga cgtcccaaaa aggacaaatc ggtaaaatcg
     1261 tcaaatcgcg cgcctggaag cttggggtaa ctggccaatt ttggggattt taggaggaag
     1321 gataattcgc cgctgagtat tatagaaact atgcggcact cgttcctggt gtctcggcct
     1381 gtgactcttc gctagtaagc cggggcaatt atccctgatc gcgtaaatcc gtcactacac
     1441 cttttgagtt ggcacctggg agacttgtct catgtcgttt cggcggccgt ccagggcgac
     1501 acgtcgcctt gtgattgagt cacttgagca gcgtcatctt ctttcggtga catcgcttcc
     1561 gctgcccggc caattcctcg atctgggccc tgtggactat tcccaggtga cccttgcaga
     1621 ttcgtcttcg gcggggcagt ggttccgact aaccgcccaa aacacggcga cgttgaccgc
     1681 gatttgcggt ccgtcaccgg ctgatcccag cccgcttggt gttacggtgt acgcgctcag
     1741 cggtaacgca ctggtggaac ttgctcgggg taacggcagg gtggacacac ctgtcactgc
     1801 cggatcgacc tacttcgtct ggcttggtca ggttcaaagt ggggaacaat tgttccttgc
     1861 taaccttgtt caaaactccg ggacaaccgt aactgtcgtt ggaacggccg gtgatgacac
     1921 gtttgagttc gacggtacca gccgg
//
LOCUS       DAOPTR010000823         1943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_46113_length_1943_cov_3.698093, whole genome shotgun sequence.
ACCESSION   DAOPTR010000823 DAOPTR010000000
VERSION     DAOPTR010000823.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46113_length_1943_cov_3.698093"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PKI05_13545"
     CDS             <1..576
                     /locus_tag="PKI05_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53272.1"
                     /translation="VFLEAVRTGDDERVVALFSEEARKQAGQLNRQFAPVGSDTARYE
                     VFEDVQYLAPDGARVRTHWTDLDSRGQPRTDEITWMLRKEANGWRVAGMATVIFEGEP
                     PLLLDFENMQETLRKVEMLSEEIERRQEAAQKVAAKQEWEDAGAENQASGQLPNSPQQ
                     DPSAADLPAAPGVNDGSTMTALKPDEPITRE"
     gene            complement(623..1492)
                     /locus_tag="PKI05_13550"
     CDS             complement(623..1492)
                     /locus_tag="PKI05_13550"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOA53273.1"
                     /translation="MPLTRREMLTSVGGLLASSSLRPLLADQNRLYKIGACDWSIGAR
                     QDIRAFEVAKEIGLDGVQVSFGEPGGSYDLRDQKVRQQYAEAAAKTGVEIASLAMGVL
                     NNIPFSSDPRTVEWVSDCIDVMAAMGQKIVLLAFFGKGDIKGKRDLQDEVIRRLKQLA
                     PKAEEKQVILGVESTLNVDDHLRILDGVGSPAVQVYYDVANMHYAGYDIFAELRRLGR
                     ERICQIHMKEYNHLLGQGPIDFLKIRDVLGEIGYYDWLIIEAATVKGRSVVDCYRDNQ
                     KFLRSLFPTAKTA"
BASE COUNT          463 a          543 c          536 g          401 t
ORIGIN      
        1 gtttttcttg aggcggttcg cacgggagat gacgaaaggg tggtagcgct cttttcagaa
       61 gaggctcgca agcaggcggg ccagctcaat cgccaatttg cgcctgtcgg tagcgatacc
      121 gcgcggtatg aggtttttga ggacgttcaa tacctggcgc cggatggagc ccgcgtgcgg
      181 acgcactgga ccgatctcga ttcacgcggt cagccccgca ctgatgaaat cacctggatg
      241 cttcggaaag aggcgaacgg atggcgcgtg gcgggaatgg ctacggtgat attcgaaggc
      301 gagccgccac ttctcctcga ttttgagaat atgcaagaga ccctccgtaa ggtcgaaatg
      361 ttgtcggagg aaattgaacg ccggcaagaa gcggctcaaa aggtggcggc caagcaagaa
      421 tgggaagacg ccggagcaga aaaccaggcg tcaggccaac tgcccaacag cccacagcaa
      481 gacccaagcg cagcagactt gcccgccgct ccgggcgtca acgacggctc gaccatgacg
      541 gcccttaagc cggacgaacc catcacaaga gagtaacgaa tcgggtgact ccgccccaag
      601 ccggcgccgc caggacctcg tgctacgccg tcttggccgt tgggaataac gaccgcaaga
      661 acttctgatt gtcacggtaa cagtccacca cagatcgccc tttaaccgtg gcggcctcaa
      721 taatcaacca gtcgtaatag ccgatttcgc cgaggacatc acgaatcttt aggaagtcga
      781 ttggtccttg gccgagcaaa tggttgtatt ccttcatgtg aatttggcag attcgttcgc
      841 gtcccagacg gcgtagctcg gcgaagatgt catatccggc gtaatgcata tttgccacat
      901 cgtaatagac ttggaccgcc ggtgaaccca caccgtctag aatcctgagg tggtcatcca
      961 cattgagcgt gctttcgact cctaagatta cctgcttttc ctcggccttt ggagcaagtt
     1021 gtttaagtcg ccgaatgacc tcatcttgta aatctcgttt acctttgata tcgcccttcc
     1081 caaagaaggc caaaaggaca atcttctggc ccatggcggc catcacatcg atgcaatcgc
     1141 tcacccactc tacggttctt ggatcgctgg aaaacggaat gttgttgagc acacccatgg
     1201 ccaaggatgc gatctccacc cctgtcttgg ccgcggcttc cgcgtactgt tggcgaacct
     1261 tttggtcacg gaggtcatag ctcccgccgg gttcgccgaa actcacttgt acaccgtcca
     1321 gaccaatttc cttggcaacc tcgaaggcac gaatatcctg tctcgctccg atggaccaat
     1381 cgcaggcacc aattttgtat aggcggttct ggtctgcgag caatggccga agtgaagaac
     1441 tggccaacaa acctcccact gacgtgagca tctcgcgacg tgtcaacggc atcgttgagt
     1501 ctccctgcaa gcagctcaaa agggacaact tcttgcaaac acgaaatcta gtttaatcca
     1561 agggcgcggg cattactatc caacgagaag acacccgaag gttcgtggga aaaaacaaaa
     1621 aattaagagc ctatcccata atctctcgtc acccgtacgg cgggagaggg gtcgggggtg
     1681 agggcggctc gaatcgctga aggttttctc gatcgcccca aacgatcgat tgccctcacc
     1741 ccaaccctct cccagaggga gagggggaca tttgcgatag actttaaggt aggggttact
     1801 ggttggcaag ccagggtttg gagcgaacgc gttcaggttc actcaaaggg tgctcaaagc
     1861 cgggaggccg tccaccgtcg atcgcagggg cagtgcggtc ctcgggatac gggtcgaaac
     1921 ctttccaata agacgtcatc gac
//
LOCUS       DAOPTR010000824         1937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_46325_length_1937_cov_2.615303, whole genome shotgun sequence.
ACCESSION   DAOPTR010000824 DAOPTR010000000
VERSION     DAOPTR010000824.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46325_length_1937_cov_2.615303"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..>1937
                     /locus_tag="PKI05_13555"
     CDS             98..>1937
                     /locus_tag="PKI05_13555"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HOA53274.1"
                     /translation="MATLGTTLLGLGQHSQLLPALALVSSIVSFVFTDLLRVLVLRQW
                     MTNLLMIIALSVVAVDLARASGFVNVLAMADLLVYLQCILQFQTKNTRVFRYLVTLSA
                     FQVVSASAFYHGVLFGTLLIVYLLNSLIVMVMLAFYEVWQKQMEHHKEGSQPQTVSHR
                     LVGDRIPDSSPLGFNGRLLARTLFLGVGSLAFALVVFMVVPRLSSRPWPGLSPAPLRT
                     VGFDRQIALGELGFLVEDSTPVMRVEVTDMDGNPATVMAPVYLRGGSYTFYRNGQWET
                     NQFIVGGEVQNLKPLYRGPGQSLHRQRIILEPLDTSDVFGIWPYGRWDDAPLVYDPLL
                     TRLFRLSDRPSRISYEMTTSAIENERLVDIVPATEPVAREQYLQLPWVRVVPTWLGPR
                     FEVDRTVYELRGWDRVVKLARQWAAESPWPANDHFHTARFFEQQLAYSGYYRYSLAGV
                     TRNATLDPVVDFLTEHPEGHCEYFATALALLLRCQGIPTRIVIGYKTYDYNTLGHFYQ
                     VRQRDAHAWVEVYLAPNQITQDLVGERPLELFRFGAWLRLDPMPASALGLASSQGPWQ
                     RFWNAYNFLDYIWLKYVMQMDQPRQETEIYRPVRDRVRKQVDWFLDL"
BASE COUNT          381 a          535 c          505 g          516 t
ORIGIN      
        1 gaccaattct aagctggtta tgagtccctg actacttgca atggccttga gttcggtacg
       61 acgcctggaa ttgagtctcc aactcagtgt cgcatgcatg gcgacactgg ggaccactct
      121 tcttgggctc ggacaacata gtcaactact gcccgcactg gctttagtct cctccatcgt
      181 gagcttcgtg ttcactgatc ttctaagggt gcttgtcctc cgccaatgga tgaccaatct
      241 cctcatgatt attgccctga gcgtggtggc cgttgacctg gcccgtgcct cgggctttgt
      301 caatgtgttg gccatggccg atttgctggt ttaccttcaa tgcattcttc aattccaaac
      361 gaaaaacacc cgtgtgtttc gctacctggt cacgctaagc gccttccaag tcgtttccgc
      421 gagtgcgttt taccatggcg tcctttttgg aacgctgctc atcgtttatc tcctcaatag
      481 cctcatcgtg atggtgatgc tcgcgtttta tgaagtttgg caaaaacaaa tggaacatca
      541 taaggaaggg agtcagccgc aaaccgtctc gcatcgcctg gtgggtgacc ggatacctga
      601 ttcaagccct ttggggttta acggccgact gcttgcacgg acattgttcc tgggtgttgg
      661 ttcccttgct tttgctttgg tcgtcttcat ggtcgtacca cgcctgagtt cccgtccgtg
      721 gccgggactg tcccctgctc ccctacggac cgttggtttt gatcgccaaa tcgccctggg
      781 agagcttgga tttctggtgg aagactcaac acccgtcatg agggtggaag tcacagatat
      841 ggacggaaac cccgctaccg tgatggcccc cgtctatctg cgtggaggat cctatacgtt
      901 ttaccgcaac ggacagtggg aaacgaacca atttatcgtg ggcggcgagg ttcagaatct
      961 caaacctttg taccggggtc ctggtcaaag tctgcaccgc cagcggatta ttcttgaacc
     1021 tttggataca tccgacgttt tcggtatctg gccgtacggc agatgggacg atgccccttt
     1081 agtttatgac cccttgctga cccgtctatt tcgtctttcc gatcgaccta gccgaatcag
     1141 ttacgaaatg accacgtcgg caatcgagaa tgagcgactt gtcgatattg ttccggctac
     1201 cgagccagtg gcccgcgaac aatacctgca acttccctgg gttcgagtcg ttcctacttg
     1261 gttgggacca cgtttcgagg tggatcgaac agtttatgaa ttacggggct gggaccgtgt
     1321 cgtcaagttg gcgcgtcaat gggcggcaga gtctccctgg cctgccaatg accatttcca
     1381 cacagcccgc ttctttgaac agcaactggc gtattccggc tattatcggt actctttggc
     1441 gggggtcacg cggaacgcca ctcttgaccc ggttgttgat ttccttacgg aacacccgga
     1501 aggacactgt gaatactttg ccactgccct ggctttactg ttgaggtgcc agggaatccc
     1561 aaccaggatc gtgatcgggt acaagacgta cgattacaac accctcggtc atttctatca
     1621 ggtccgtcaa cgcgatgccc acgcctgggt ggaagtctat ctagcaccaa atcagattac
     1681 tcaagacctg gttggcgaaa ggcccttgga acttttccgt tttggggcat ggcttcgcct
     1741 tgatccgatg ccggcctccg cactcggttt ggcttcctcg cagggtccct ggcagcggtt
     1801 ctggaacgct tacaacttcc tcgattacat ctggcttaag tatgtcatgc aaatggacca
     1861 accccggcag gaaacggaga tttaccgtcc tgtccgggat cgggtccgca aacaggtcga
     1921 ttggtttctt gatctcc
//
LOCUS       DAOPTR010000825         1933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_46439_length_1933_cov_3.825879, whole genome shotgun sequence.
ACCESSION   DAOPTR010000825 DAOPTR010000000
VERSION     DAOPTR010000825.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46439_length_1933_cov_3.825879"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(315..1772)
                     /locus_tag="PKI05_13560"
     CDS             complement(315..1772)
                     /locus_tag="PKI05_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53275.1"
                     /translation="MTQVVLTATYFGFLLVIWLAGRMWLRLFSSLFSQCPRDDQPGNA
                     EAFLVGSAMLGLASYLVIAIFGRMPPLGYCLLVAVHCAMALVGWCVARRPAGPQWKRG
                     LKTAFFIWAVILGLYAAQLPLTGYDARAIYGLKAKIVADGANLWGPDFRDPYRLHFGS
                     NYPLLIPILESFLFRFRMILSGGDPWGDVGLPLIFYGFIVAGTLLVTKVTERLAPGWG
                     IFSGVIWAITPMVWRWTEGAGLSGSADLPFAVFLAAGVFHLVEGWSSQDRFSLLLAGI
                     FFGATILIKQEGLIGLGLGTVTIFVDQILWQRSRQKDNTPMVPNRGPSKLASFGVFLV
                     ILIPFLLFHRLVHAEMPQQIYMRSYAAALSWEWLQQITDRPATILSFAMKELINNHWG
                     LAWPALVVALLLKRDRPLSPTVRRIRLFVLLLMAAYTGIFAVTPFPLLYHLHTAYARL
                     MLHALPLAIVVLVEQLAASLDFTHFGHGNTPESTR"
BASE COUNT          485 a          507 c          550 g          391 t
ORIGIN      
        1 gggtggcttc gagcacgcag ctctggggca aaaggtacac cttagagctt atcccacgac
       61 gtcccctctc ccgctcggcg ggagaggggt cgggggtgag ggtagctcga atcgccgctg
      121 gtttttttat ccactccaac cattgcttgc cctcacccca accctctccc agagggagag
      181 ggaattttcc gataggctct cagctatttt gtggtttggc agcgagctgc atctaaaatc
      241 ctgtgccctc gactgttcaa gccgcacgta gttgccggat ttgtttactg ttgcgcgagc
      301 gaagaatttc cgcatcacct ggtgctttcc ggcgtgttgc cgtgtccaaa gtgggtaaag
      361 tccagactgg cagccaattg ctccaccaat accacaatgg ccaagggcag ggcgtgaagc
      421 atcaatcgtg cataggcggt gtgcaggtga tacagtagag ggaaaggtgt cactgcgaag
      481 atgccggtat aagctgccat tagaaggagc acaaacaagc ggattctccg cacagtgggc
      541 gaaagagggc ggtccctttt caacaaaagt gccacaacca aagccggcca ggctagtccc
      601 caatggttgt tgatcagttc tttcattgca aaagacaaaa tggttgcggg tcgatccgtg
      661 atttgttgca accactccca agagagagcg gcggcatacg atcgcatgta gatttgctgg
      721 ggcatctccg cgtggaccag ccggtggaaa agaagaaacg gaatcagtat cacaagaaag
      781 acaccaaaag aagcgagttt gcttggacca cgattgggca ccataggagt gttgtccttc
      841 tgacgcgatc gttgccacaa gatttggtcc acaaagatcg tgactgttcc aagtcctagg
      901 ccgatgaggc cttcttgttt gatcaagatt gtggccccga aaaagatccc ggccaagagt
      961 agtgagaaac gatcttgtga ggaccagccc tcaacgagat ggaagacgcc tgcggccaag
     1021 aaaacggcga aaggaagatc ggccgatccg gagagccctg ccccttccgt ccatcgccag
     1081 accatgggag ttatggccca gatcacgccg ctgaaaatcc cccaacctgg tgccagccgc
     1141 tctgtgacct tggtcacgag tagtgtcccc gccacgatga atccatagaa gattagcggc
     1201 agacccacgt cgccccatgg atcgccaccg cttaagatca tgcgaaaacg aaaaaggaag
     1261 ctttctagaa tggggattag caggggatag ttactgccaa agtgtagccg atagggatcg
     1321 cggaagtctg ggccccaaag attggcccca tctgccacta ttttggcctt aagcccataa
     1381 atggcccggg catcgtagcc ggtcaacggc aattgggccg cgtaaaggcc caggatgact
     1441 gcccaaatga aaaacgcggt ctttaaacct cgtttccatt ggggaccggc aggccttcgg
     1501 gcgacacacc atccaacgag agccattgcg cagtgaaccg ccacgagtaa acagtagccc
     1561 agcggtggca tccgaccgaa gatggcgatc acgaggtaac tggccagccc tagcatggca
     1621 cttccaacga ggaatgcttc tgcgttaccg ggttggtcat cgcggggaca ttgcgaaaaa
     1681 agagaggaaa atagtcgcaa ccacatccgg ccggcgagcc agatgaccag aagaaaaccg
     1741 aaataagttg ccgtgagaac tacttgagtc attgggggga tgataggggc ggcgtcgagt
     1801 cctttttcaa gccttcctga cgtaaagaat ccggcattgc ttgtaatgag cagccctttt
     1861 cgtctgcttc gtcgaatgtc aacaggaact cgatgtggcg ctgctggatg aacgtctcga
     1921 ggtcgagact ggg
//
LOCUS       DAOPTR010000826         1930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_46541_length_1930_cov_3.225600, whole genome shotgun sequence.
ACCESSION   DAOPTR010000826 DAOPTR010000000
VERSION     DAOPTR010000826.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46541_length_1930_cov_3.225600"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..1216
                     /locus_tag="PKI05_13565"
     CDS             59..1216
                     /locus_tag="PKI05_13565"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOA53276.1"
                     /translation="MRCLQPRRSAGPVCQPPGLLTVVLCLWAGLASGSETGTRPTQLE
                     RVKVSADGRGFVLVPSGRSFVPWGFNYDHDAKGQLLEDYWHTDWDRVVGDFREIKALG
                     ANVVRIHLQVARFLEAPDRPNKENIERLKRLFQLAEELGLYLDVTGLGCYHRQDIPAW
                     YDALDEEARWAVQAFFWETIAQAGAGHPAVFCYDLMNEPVVPVDKRKAGDWLVDAALG
                     GKYFVQRITLDPRGRARKDIARAWVDRLATAIRKHDTDALITVGLLPGTKERADAWSG
                     FVPSELIPPLDFIATHIYPESEKLDVSLQILSHFSVGRPVVVEEVFPLRCNTQELKGF
                     LRASRQYASGWIGFYWGVPPDELRQSKELAAQITAAWLELFQELAPEFRQP"
     gene            1435..>1930
                     /locus_tag="PKI05_13570"
     CDS             1435..>1930
                     /locus_tag="PKI05_13570"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOA53277.1"
                     /translation="MSLTDTALPKAASELRLQPRKSSATVFGPYLCLAAALLYAGSNM
                     ALRRLAEPGQAADPIWVLFVKESITVLVVGPWLLCQSTRGRIRLPSQGNFLAIIAAAI
                     PTQVVANLGMLWAFGQVGIAIAVPVSLAVNLITAAILGRFFLSERVSPRTLLAMLLLI
                     VGISV"
BASE COUNT          380 a          519 c          542 g          489 t
ORIGIN      
        1 acggcagtac ggtatttact gctttggatg aaggtgttgt tttccagcgg agatcggtat
       61 gaggtgtttg cagccccgac gttccgcagg tcccgtttgc cagccgccgg gactgctgac
      121 ggttgtatta tgtctctggg ccgggctggc aagcggttct gaaacgggaa ctcggccgac
      181 acaactcgaa cgggtgaaag tctctgcgga cggccgcggg tttgtgctcg tcccgtcggg
      241 acgaagtttc gtcccctggg gctttaatta cgatcacgat gccaaaggcc agcttcttga
      301 ggactattgg cataccgatt gggatcgggt ggtcggtgat tttcgggaga tcaaggcctt
      361 gggggctaac gtcgttcgta tccatcttca ggtggcaaga tttctggaag ctcccgaccg
      421 tccgaacaaa gagaatattg aaaggcttaa gcgacttttt caattggccg aggagctcgg
      481 gctgtatcta gatgtcactg gcctgggctg ttatcaccgg caggacattc ccgcttggta
      541 cgatgccttg gatgaagagg cgcgctgggc ggtccaggcc tttttctggg agaccatcgc
      601 ccaagccggg gcgggccacc ccgcggtctt ttgctacgac ctgatgaacg agcctgtggt
      661 tccggtggat aaacgcaagg caggtgattg gctggtggat gctgccctag gcggcaaata
      721 ttttgtgcag aggatcacgc ttgaccctcg gggacgagcc cgcaaagaca tcgcccgcgc
      781 gtgggtcgat cgcctagcca cggccattcg caagcacgac accgacgcgc tcatcacggt
      841 agggcttttg ccggggacaa aggagcgggc ggacgcttgg tcgggctttg taccgagtga
      901 attgattccc ccgttggact ttatcgcgac tcacatctac ccagaaagcg agaagctcga
      961 cgtctcatta caaatcttat cccacttttc cgttgggcga ccggtggtcg tggaagaagt
     1021 ttttcctttg cgatgcaata cgcaggaatt gaaaggattc ctccgtgcct cgcgtcaata
     1081 tgcctcaggt tggatcggct tttactgggg cgtcccgccc gatgagttgc gccaatccaa
     1141 agaacttgcc gcccagatca cggcagcttg gttggaactt tttcaagaat tagcgccgga
     1201 atttcgccag ccgtgatcac gctggttgcc gcaatgggca atctctcgcc gttgatttta
     1261 acagaggctc gtccactgcc agcctatgag tctttacgat atcagttgaa atgtttgcga
     1321 gacttccaga tagaataaac aaaatctcac tactttttta ggtagccaca cctgacaatt
     1381 gtttctttgc tacaatgatg tttttgaaga tttaatggtg taggatcccg ccgcatgtca
     1441 ctgactgata ccgccttgcc aaaggccgca tcggaattgc ggttacagcc acgcaagtct
     1501 tcagcaaccg tctttggccc atatttatgt cttgcggcgg cattgctcta tgcaggatca
     1561 aatatggctc tgcggcggtt ggcagagccg ggccaggccg ctgatcccat ctgggtcctc
     1621 tttgtgaaag aatccatcac tgtacttgtg gtgggaccgt ggcttctctg tcaatccacg
     1681 cgtggacgaa tccgcctacc ttcccaagga aatttcctcg ctatcattgc cgcggccatt
     1741 cccactcaag ttgttgccaa tctgggaatg ctctgggcgt tcggccaggt cggcatcgcg
     1801 atcgccgtac cggtttcttt ggctgttaac ctcattaccg ctgccatcct cggtcgattc
     1861 tttctctccg aacgcgtatc ccctcggaca cttcttgcca tgctgctcct catagtcggg
     1921 atcagcgtga
//
LOCUS       DAOPTR010000827         1927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_46629_length_1927_cov_3.191774, whole genome shotgun sequence.
ACCESSION   DAOPTR010000827 DAOPTR010000000
VERSION     DAOPTR010000827.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46629_length_1927_cov_3.191774"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PKI05_13575"
     CDS             complement(<1..322)
                     /locus_tag="PKI05_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOA53278.1"
                     /translation="MAKSAARQFAIGLDYGTNSVRALIVDVRTGEEVGTAVYQYPSGD
                     KGVLLDPKNPNLARQNPADYIEGFYRCVRAAIRSVPKSVGFAPEHVVGIGVDTTGSTP
                     IPVDE"
     gene            complement(672..1325)
                     /gene="trxA"
                     /locus_tag="PKI05_13580"
     CDS             complement(672..1325)
                     /gene="trxA"
                     /locus_tag="PKI05_13580"
                     /inference="COORDINATES: protein motif:HMM:NF025105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOA53279.1"
                     /translation="MKRNQIWQKSGYLTRYEVLSVKLRTVGAFVLAGGLTILGGCTSE
                     ALSGSHLRPESISETLAVTPADQNSVRRSGEGDAISRETTKTDQPFRDTSTNPSQEKK
                     ETRTMWFTGGKNTIEHANAANFNTLVAQSSVPVLVDFYADWCGPCRALAPILEQLAQE
                     VNGVRIVKVNVDQAPTLAAQYRVSAIPTLILFDQGRPVQRITGLASKEDLKRLLALD"
     gene            1720..>1927
                     /locus_tag="PKI05_13585"
     CDS             1720..>1927
                     /locus_tag="PKI05_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53280.1"
                     /translation="MMHLTVARSRVDFVFISAHDKALRLKVATRKWECRFLTISPIWP
                     TQFGVCGPPGGDNIPYAGGSKRVFF"
BASE COUNT          449 a          518 c          510 g          450 t
ORIGIN      
        1 tttcatcaac cggaatgggc gtagaaccgg tcgtatcaac cccgatgccc accacatgct
       61 ctggagcgaa tcccacactc ttcggcacac tgcggatggc cgcccgcacg cagcgataaa
      121 acccctcaat atagtccgcc gggttttgcc gcgcgagatt cggattcttc ggatcgagga
      181 gaacgccttt gtcaccgctg gggtattgat aaactgcggt tccgacctct tcgccggttc
      241 gcacatccac aatcagcgcc cgaacactgt tggtgccata atcaagaccg atcgcaaact
      301 ggcgtgctgc cgatttggcc atgcttaagg ctcctttatc acatcaccac ggttgcacag
      361 atgagctatc tggaacaaac actcggcaaa tcatttggat aagactaccg tcctacccgt
      421 tccgctgcaa caggagcgtc tcacaattgt ggactgtggg aaacgcaacc aaatggcggg
      481 ttgccagtgg ctctgtgggg acttctcgca gacgctgttt tgaagcgcat caaaacgacc
      541 tccgcgataa gcggaggtcg caacacagga tgcgatgaat gatcttaaaa ccccaacaat
      601 acgattcccg actagccccc gcacgtccgc aagctacaga actacagcta cagaactacg
      661 gatcttggtc gtcaatcgag ggccaaaagc cgcttcaaat cctccttgct agccaaacca
      721 gtgatacgtt gaaccggcct tccttgatcg aagaggatga gcgtgggaat ggccgagaca
      781 cgatactggg ccgccaaggt gggggcctga tcaacattca ctttgacgat ccgaacgccg
      841 ttaacttcct gggccaattg ctcgagaatt ggtgccaggg cacggcaggg accgcaccag
      901 tccgcgtaaa agtcaaccaa cacagggaca ctggattggg ccacaagggt gttgaaattt
      961 gctgcgttgg cgtgctcgat ggtgtttttt ccaccggtaa accacattgt tcttgtctcc
     1021 tttttctctt gacttggatt tgttgaagta tcacgaaacg gctgatcagt tttggttgtt
     1081 tccctcgaga tcgcgtcgcc ctcgccggac ctcctcacgc tgttctgatc cgccggagta
     1141 acagcgaggg tttcggaaat cgattcgggg cgaagatgcg agccggaaag ggcctcactg
     1201 gtgcatcctc cgagaatggt aagtcctccg gcgaggacga atgccccaac agtccgtaac
     1261 ttcacggaaa gtacctcata acgtgtgagg taacctgact tttgccaaat ctgattccgc
     1321 ttcaaggtca aacctccatg acatgtaaca cttatttgac tttggcgtga catcctgatc
     1381 acgaaccctc aaagggtctg atgccttccg tcctaggaag gttacaaagg gaaggggtcc
     1441 actttgggac ctggctaatc ggtcacctcc gcttgaaact accggtggct tttcatgaca
     1501 aagcgctggt gataaacaga atcgtgagcc gtattttcat agtcctgaac acagtggcgg
     1561 ctgggcggat gcgaagaaac gagagaatct ctccgaggta gcctttgtta aaacggagat
     1621 gtcacatgat tttgtgcctt tcgtgtcttg ctaccttgac ttggccccaa gaactgggcg
     1681 acaatgaaag gcgtcgacac ctgtttccgg ggcggcggga tgatgcactt aacggtcgca
     1741 cggagcaggg tcgattttgt ctttatttct gcccacgaca aggccctgcg attgaaggtt
     1801 gcgactcgca agtgggagtg tcggtttttg acaatatcgc caatttggcc aacacaattt
     1861 ggtgtctgtg gtccaccggg cggggataat atcccctatg cgggcggttc gaaacgggtg
     1921 ttttttc
//
LOCUS       DAOPTR010000828         1920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_46845_length_1920_cov_2.945308, whole genome shotgun sequence.
ACCESSION   DAOPTR010000828 DAOPTR010000000
VERSION     DAOPTR010000828.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46845_length_1920_cov_2.945308"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PKI05_13590"
     CDS             complement(<1..447)
                     /locus_tag="PKI05_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53281.1"
                     /translation="MNVLWALPPAEKLFPDTARGFLAVRDVSELREHFDQTQIGQLVK
                     DPALRPFIEDIRLQIRQRLVGAQQELGISLEDVAQLATGEVAVGLVFAEDAERPALAA
                     LACIKGKEKESQELFDRITEEMKKQKATVTKTSVAGKEVWAIKLAED"
     gene            complement(948..1769)
                     /locus_tag="PKI05_13595"
     CDS             complement(948..1769)
                     /locus_tag="PKI05_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53282.1"
                     /translation="MSMSQDLVARNVILVLLVCWFSIVSQNVGLAESSNSNAAAPIKS
                     ATAPPYRPTAEYHQREMRGWRLMVAPDLLEDEKLANAVFAELERQLAAIEQVVPAGPL
                     EKIRQVVIWVEKYESHHPCMAYHPDRNWLLEHDMNPDKARSVEIANARNFIRWTREQP
                     WMVLHELAHGYHHRFLPGGYRNKEIREAFERASREGKYESVAYVRGGEKRAYALNNPM
                     EFFAENSEAFFGKNDFFPFTRADLEKFDPETFALLQKLWEVPEESTQERENAATR"
BASE COUNT          417 a          580 c          464 g          459 t
ORIGIN      
        1 atcttccgcg agcttgatag cccaaacctc cttcccggcc acagaagtct tggttacggt
       61 cgccttttgc tttttcatct cctcggtgat acgatcgaac aattcttgag attctttctc
      121 cttccccttg atacaagcca gggcagcgag cgctggccgt tcagcatctt ccgcaaagac
      181 caggcccaca gccacttcgc cggtggccag ttgtgccaca tcttcgaggg aaattcccag
      241 ctcctgttgc gcgcccacca aacgctgtcg aatctgaagc cgaatatcct cgatgaacgg
      301 gcgaagggct gggtccttga ccaattggcc aatttgggtc tggtcgaaat gctctcggag
      361 ctccgagacg tcgcgcacgg ctaagaaacc gcgcgccgtg tcgggaaaga gcttttccgc
      421 cgggggcaac gcccaaagga cgttcatcac actccaagca accacaccga acagaacttc
      481 acaaaacgct cttgtctttg atcccatgtt ctcaaccact gctgtcaaaa agagaaacgg
      541 ctacccacac cgatctcgcc ggagccgcaa agtttggcgg ggccccagtc tgtccggttt
      601 atactgagaa tcgacacaat cgcagtattt catcttttcg ttaatatcga cccgagatgt
      661 tgacgaacga tactaaacac tcaagattcc cattttttcc gcatatttcc ccgtcaccaa
      721 ggtccggaat gtcagccgat tttgcgggct ttagctctcc tctcgcacca tgcatagatc
      781 atgcctaaga tttgggcatt caacggatta ccggccgctc ttcatttcac gtcctgctta
      841 gggagacggt gacccaaagc cccgcacgcc agccgctttt cgcctctcga ggagattctt
      901 aaggcggcct gcgacatatt tgaacagcta tgagttgccg gcacggatta ccgagtcgcc
      961 gcattctccc tttcttgcgt agactcttcc gggacctccc agagtttttg gaggagggca
     1021 aacgtctcgg gatcaaattt ctcaagatcg gcccgagtga aagggaagaa gtcattcttt
     1081 ccgaagaatg cctcactatt ctccgcgaaa aactccatgg gattatttaa ggcgtaagcc
     1141 cgtttttcgc caccccgcac ataggccacc gattcgtatt taccctctcg gctggccctt
     1201 tcaaaggcct ctcggatctc cttgtttctg taaccaccag gaaggaaccg atggtggtaa
     1261 ccatgggcga gctcgtggag taccatccac ggttgttccc gcgtccagcg gataaagtta
     1321 cgggcattgg cgatctccac cgagcgggcc ttgtccgggt tcatatcatg ttcaaggagc
     1381 caatttcggt cagggtgata tgccatgcac gggtgatgac tttcatactt ctcgacccaa
     1441 atgaccacct gtcggatttt ctcgaggggc cccgccggca ccacctgctc gatggccgca
     1501 agttggcgtt ccagttcagc aaaaacggca ttcgccaact tctcatcttc caaaaggtcc
     1561 ggggcgacca tcaatcgcca tccccgcatt tcccgctggt gatactccgc cgttgggcga
     1621 tagggtggtg ctgttgcgct cttaatcggc gcagccgcgt tagaattgga gctttctgcc
     1681 agtccaacgt tctgggaaac aatgctgaac caacaaacaa gcagcaccag gattacattc
     1741 ctcgcaacga ggtcttgtga catgctcatc agagcctcca gccctctcga tacggtttgt
     1801 tgatccacgg atcggcctcg gggcaattga ttgccttcaa atttacggca tcccagtgaa
     1861 ttttctgtcc cacccggaat gccaccgttc ccagcagggc cgcttccgtt aagggccccg
//
LOCUS       DAOPTR010000829         1918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_46906_length_1918_cov_3.723564, whole genome shotgun sequence.
ACCESSION   DAOPTR010000829 DAOPTR010000000
VERSION     DAOPTR010000829.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46906_length_1918_cov_3.723564"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(313..513)
                     /locus_tag="PKI05_13600"
     CDS             complement(313..513)
                     /locus_tag="PKI05_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53283.1"
                     /translation="MLTAVWQDVATWLSIALAAIYLVHHFRRQPGMGASRCGGCTHCP
                     MSDALQEWAAAWRQTNHNGQDQ"
     gene            complement(574..>1918)
                     /gene="feoB"
                     /locus_tag="PKI05_13605"
     CDS             complement(574..>1918)
                     /gene="feoB"
                     /locus_tag="PKI05_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOA53284.1"
                     /translation="TLIFVLVMLLMFQAVFRLAAPLNEAIDSAIQGIAGWLTAEMAPG
                     AFRSLLVDGVISGVGAVLTFIPQIAILFLFVAVLEDCGYVARAAFLMDRFMSRIGLSG
                     KSFIPMLSSFACAVPGIMATRTIDNERDRLTTILVAPLMTCSARLPVYALLIAVFIPD
                     QRYLGGLLGLQGLVLASLYALGIVTAVVVAITLKTTLFRGRSPTFVMELPSYKWPSPR
                     TIILRAWERVVVFVRNAGTIIAAISILVWAALYYPHDLRSLPPELLRRQQELIVLRDT
                     LPPGSSQRQRWETELEQIERQITAAYQRSSFMGRLGRAIEPVFRPLGWDWRIGTAVIA
                     SFPAREVVVATLGVMFNLDADVAEANQGPSPFHDRLRSAVWEGTDRPLFNIPVALSLM
                     VFYALCAQCAATLAVIGRETNSWRWPIFTFAYMTTLAYLGALATYQIGARIMTGT"
BASE COUNT          445 a          570 c          563 g          340 t
ORIGIN      
        1 aaacggggca accacaggtg gttgccccta cggttgacgt tatggccgcc cggaaggcga
       61 actgaaattg cgtggcacgg gcatcctgcc cgtggtattc ttcatcccaa cgttaagcgc
      121 ttgtctgcaa cgggcgtccg tgttaccaga tgccagcgtc atcggggacg atgaccctcc
      181 cgctgcggaa ttgggaggga ccgcgtcctc gccgtcccac gttgacgcca ggtaccggtc
      241 acctgaatcg cgggagcaaa cagtttcaga taaggttcga atcaggatgg cgggcggggt
      301 gtcgatctct acttactgat cctggccgtt atggttcgtc tgcctccacg cagccgccca
      361 ctcttgtaat gcgtcactca ttgggcaatg cgtacagccc ccacaccgtg aggcccccat
      421 tccaggctgc cgccgaaagt gatggacgag atagattgcc gccagggcaa tggacaacca
      481 cgtcgcgacg tcttgccaaa ctgccgtcaa catcgcggag accctttgtt cgatccgacc
      541 aattgggggt ataatgtaat cagcgagaac gaatcatgtg cctgtcataa ttctagcgcc
      601 aatctggtac gtggcaaggg cgccgaggta ggccaaggtt gtcatgtacg cgaatgtgaa
      661 aatgggccag cgccagctat tggtctcccg tccgatgaca gccaaggtag ctgcacactg
      721 ggcgcacaac gcataaaaga ccatcaatga aagcgccaca gggatattga aaagcggacg
      781 atctgtgcct tcccaaaccg ccgatcgcaa gcggtcgtga aaaggcgagg ggccctggtt
      841 ggcctccgct acatcggcgt ccaagttgaa catcacgccc agggtggcca cgacaacctc
      901 gcgggctgga aaggaggcga tgacggctgt gccgattcgc caatcccatc caaggggtcg
      961 aaacacgggt tcgatggccc ttccgaggcg tcccatgaag ctgcttcgct gataggccgc
     1021 cgttatttgc cgttcgattt gctccaattc cgtttcccac cgctgtcgct gcgaggatcc
     1081 cggcggcaag gtatcccgca ggacgatgag ttcctgttgc cggcggagta actcgggcgg
     1141 caggctgcgt agatcgtgcg gatagtacag ggccgcccag accaaaatcg agatggctgc
     1201 gataatcgtc ccagcattcc gcacgaagac gaccacacgt tcccaggccc tcaggatgat
     1261 cgtccgcggc gacggccatt tgtagctggg aagctccatg acgaaggtcg gcgaacgacc
     1321 gcggaaaagc gtggttttca atgtgatggc caccacaacg gccgtcacaa ttcccagggc
     1381 gtaaagcgac gccagcacca gtccttggag tcccaacaga ccaccgaggt atctttggtc
     1441 gggaataaac acggcgatga gcagggcata gactggcagc cgtgccgagc acgtcatcag
     1501 cggtgccaca aggatcgtcg tcaatcggtc ccgctcgttg tcgatggtgc gagtggccat
     1561 gatgccaggc acagcgcagg cgaaactgga aagcatggga atgaacgatt tgccgctcaa
     1621 tccgatccgc gacatgaacc ggtccatgag gaatgcagcg cgggcgacgt agccgcaatc
     1681 ctcgagcacc gccacaaaaa gaaacaagat ggcgatctga gggataaacg tcagcacggc
     1741 ccccacaccg ctgataaccc catccaccaa aaggctgcgg aaggccccgg gggccatctc
     1801 ggccgtcagc caacctgcga tcccctgaat cgcgctgtcg atggcctcat taagcggagc
     1861 cgccaaacga aagaccgcct gaaacatcaa gagcatgacc agaacaaaga tcagcgtg
//
LOCUS       DAOPTR010000830         1915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_47014_length_1915_cov_3.548925, whole genome shotgun sequence.
ACCESSION   DAOPTR010000830 DAOPTR010000000
VERSION     DAOPTR010000830.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47014_length_1915_cov_3.548925"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..1036)
                     /locus_tag="PKI05_13610"
     CDS             complement(74..1036)
                     /locus_tag="PKI05_13610"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOA53285.1"
                     /translation="MTRPYENTTSGMTRRSFLAAGSATIVASQLLHSPWVWGAESGPL
                     HVATNFYPWLTFYRRENRDFNADLDASLGEVAAAGLNGFESGLNSPEDVDRLSPFLKK
                     HGLELRSFYVNAVMHEPEAAEKSIAQVLTVAEKAHNVMATRIVVINPSPLSWNREAPN
                     PGKNDAQLETQAKALDQLGAALRERGMQLAYHNHDMELRNAAREFHHMMLATKPENMG
                     LCLDAHWIYRGSGNSQVALFDVVKLYGSRVCELHLRQSKDGIWTEAFGEGDIDYPRLA
                     RELAALGVRPHLVLEQAVEQGSPNTMNAVEAHRRGVEYVRKLFS"
     gene            1181..>1915
                     /locus_tag="PKI05_13615"
     CDS             1181..>1915
                     /locus_tag="PKI05_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53286.1"
                     /translation="MRSLPSQLAVIGFVIGFLTMQTSLLAAEVAWPEPVVPAGFGVNI
                     HFTDPRPGEMSMLAASGLKWIRMDFVWSATEREPGKYDFRAYERLLKALDEYGIKALF
                     ILDYTNRHYDQGLSPYTDEGRQAFARWAAAAAKHFQGRGILWEMYNEPNIHFWKPKPN
                     VEDYIKLAIEVGKAIRREAPGEIYIGPATSQIDMKFLEACFQAGLLEYWDGVSVHPYR
                     QSPPETVLAEYQKLAELIAKYAPAGKK"
BASE COUNT          438 a          523 c          521 g          433 t
ORIGIN      
        1 cgcgctgttt gttgaaggaa tgcttgacgg cattttccgg tgatagcctt tcgcgagcgg
       61 cccgagggta tcttcaggaa aagagtttgc gaacatactc gacaccgcgc cggtgagcct
      121 cgaccgcgtt catcgtattg ggcgatcctt gttccacagc ctgttcgaga acgagatggg
      181 gacgaacccc gagcgctgcc agttcccgcg cgagccgcgg atagtcgata tcgccctcgc
      241 caaaggcttc cgtccaaatg ccgtctttcg attgtcgcag atgaagttcg cagacacgcg
      301 agccataaag tttcacgaca tcaaacaggg caacttgcga attccccgac ccgcgataaa
      361 tccaatgtgc atccaggcac aggcccatat tctccggctt cgtggcgagc atcatgtgat
      421 gaaattcccg tgccgcattc cgaagttcca tatcgtgatt gtgataggcc agttgcatcc
      481 cccgctcacg caaggctgcg ccgagttgat caagggcttt tgcttgagtc tccaattgcg
      541 catcgttttt gccaggattt ggcgcttccc gattccagga cagtggagac ggattgatga
      601 cgacaatccg cgtcgccata acattgtggg ccttttcagc cacggtaagc acctgggcaa
      661 ttgacttctc agcggcctct ggttcgtgca ttacggcatt gacatagaat gatcgcagct
      721 ccaatccgtg ctttttcagg aaaggtgaga gacggtccac gtcctccgga gaattgaggc
      781 cggattcgaa accattcaat cctgcggcgg caacttcacc caagctggca tccagatcgg
      841 cgttgaaatc gcgattttcg cggcgataaa acgttaacca gggataaaaa ttcgtcgcca
      901 cgtgtaaagg acctgattct gccccccaca cccatggcga atggaggagt tgacttgcga
      961 caatggtggc tgaaccagcg gccaagaaac tgcgacgtgt catccccgat gttgtgtttt
     1021 cgtacggcct tgtcataatg agattctccg ttttaggtcg ttggtgacaa atctgtctgt
     1081 caattccgaa aaagacgccc ctaatatacc gcccgccgaa accgactcgc aagatacggc
     1141 ctccacagaa gcgttaagac ataagaaagg agtcatcgcc atgaggagct taccttctca
     1201 attagctgtg atcggctttg tcatcgggtt tttgaccatg cagacctcat tgttggcggc
     1261 tgaggttgcc tggccagagc cggtcgttcc cgctggcttc ggcgtcaaca tccactttac
     1321 ggatccccga ccgggcgaga tgagcatgct tgcggcctcc ggcctcaagt ggattcgtat
     1381 ggactttgtc tggtcggcaa cagaacggga gcccgggaaa tatgacttcc gggcttacga
     1441 gcggctctta aaggccctgg acgaatatgg gatcaaagcc cttttcatcc tcgactatac
     1501 caatcggcat tatgaccaag ggttatcacc ctacacagac gaagggcggc aggccttcgc
     1561 ccgatgggca gcggcggctg ccaaacactt ccaaggacgt ggcatccttt gggaaatgta
     1621 caatgagccc aatatccatt tctggaagcc aaagcccaac gtggaagatt acatcaagct
     1681 ggcgattgag gtgggaaagg ccattcggcg ggaggcccct ggtgaaattt acattggacc
     1741 ggccacctcg caaatcgata tgaagttcct ggaggcctgt tttcaggcgg gactgctgga
     1801 gtattgggat ggcgtctcgg tccacccgta tcgacagtct cccccggaaa cggttttggc
     1861 agagtatcaa aaactggccg agttgatcgc caagtacgcc ccggcgggta aaaag
//
LOCUS       DAOPTR010000831         1913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_47070_length_1913_cov_3.992465, whole genome shotgun sequence.
ACCESSION   DAOPTR010000831 DAOPTR010000000
VERSION     DAOPTR010000831.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47070_length_1913_cov_3.992465"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..1350)
                     /locus_tag="PKI05_13620"
     CDS             complement(190..1350)
                     /locus_tag="PKI05_13620"
                     /inference="COORDINATES: protein motif:HMM:NF019239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HOA53287.1"
                     /translation="MSTRSPQKIAIRFIASLKMLGGILVTLALSSSVWAIDVVKFRGD
                     NGITEATGKILVQAVDGGVLLLTPVGVLLPLGSSEVIEITQNDLPFQAATPKEVATYW
                     LQRLPKNFEVYTTAHYVVLHNASRDYARWCGSLLERLYNGFTAYFSHKGLTIERPAFP
                     LVIVVFATRQQYIEHCREEVGDAIERIQGHYNLETNVVTTYDLTGSNGTATAAQIARY
                     IMQPGVQQTITTLVHEASHQLANNSGVTCRWTDTPQWVSEGLAMFFETPDLRSSRGWS
                     TVGALNYERLQRFRAYLPQRPSDSLATLIRDNSRFQNLSTAMDAYAEAWALTYFLLQQ
                     NGQAFITYLARLREKSPLVYDSPEERVEDFSQAFGDLRQVESEFLRYILRLR"
     gene            complement(1425..>1913)
                     /locus_tag="PKI05_13625"
     CDS             complement(1425..>1913)
                     /locus_tag="PKI05_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53288.1"
                     /translation="AVPPAVPLAEKAGQQAAPPPLGFPSEGASDPLQLVKTVARQSGY
                     VLEEFGNTLKITVPVGNLRKQVVWCSYEQSREGGQNIVRFWSVCGPASEQNAAMLLRH
                     NAQLTFGAFAFHKVNNQELIVIQMSLPANSLTIVDVARFIPALAWQADRFEESLSDQD
                     IF"
BASE COUNT          441 a          500 c          562 g          410 t
ORIGIN      
        1 ggcaagcgat cgtcggagcc gatcgagaaa accagcggcg attcgagcca ccctcacccc
       61 cggcccctct cctgccgaac gggagagggg tggttgtagg ataggctctt agtgaaaaga
      121 cgaccgagcg ctcgtcatcg cctatgaagc tgaaaaagca gggcagaggg gaacagacaa
      181 gtggcaggtc tagcgaagcc gcaagatgta gcgcaagaat tcactttcca cttgtctcaa
      241 gtcgccgaaa gcttgagaaa agtcttcaac tcgctcttca ggggaatcat ataccaaggg
      301 tgatttttct cgaaggcggg caagataggt gatgaaggct tgcccattct gctgaagcag
      361 aaaataggtc aaggcccagg cctcggcata ggcgtccatg gccgtggaaa ggttttggaa
      421 tcgtgaattg tcgcggatca acgtcgctag tgagtcggat ggtcgttggg gtaggtaggc
      481 gcggaatcgc tgcaatcgtt cgtagttcaa tgcccccacg gtgctccaac cccggctgct
      541 ccgcaagtcc ggcgtttcga aaaacatcgc caaaccctct gagacccact gcggtgtgtc
      601 cgtccagcga caggtgacac cggagttatt ggcaagttgg tgcgacgcct catggacaag
      661 cgttgtgatg gtttgttgaa ctcccggttg cataatgtac cgtgcaatct gagccgctgt
      721 ggccgtgccg ttgcttcctg tgagatcgta cgtggttacg acgttcgtct ccaggttgta
      781 gtgcccttga atccgctcta tcgcatcacc aacctcctcc cggcagtgct caatgtattg
      841 ttggcgtgtt gcaaacacca cgatcaccaa cgggaaagca ggacgttcga tggtcagccc
      901 tttgtgcgaa aagtaggccg tgaagccatt gtaaaggcgt tccaacaggg aaccgcacca
      961 gcgtgcgtaa tcccgggagg cgttgtgcaa cacaacataa tgggctgtgg tgtaaacctc
     1021 aaagtttttg ggtaatcgct gaagccagta ggttgcaacc tctttcggcg tagcggcctg
     1081 aaagggcaaa tcattctgag tgatctcaat cacctcggat gatccgagcg gaaggaggac
     1141 gccgacgggg gtcaaaagaa ggacgccgcc atccacagcc tggacgagga tttttccggt
     1201 cgcctcggtg atgccgttgt caccgcgaaa cttgaccaca tcaattgccc agactgaaga
     1261 ggacaacgcc agggtaacga ggattcctcc caacattttt aatgacgcga taaatcgaat
     1321 cgctattttt tgtggggatc gcgtggacat gggccggaaa tcacaacctg ctttcatgtg
     1381 gggcattcag ttgcaaaggc atgacccttc tggtggcgcg gcatttagaa gatatcctgg
     1441 tcggaaagac tctcttcaaa acggtccgcc tgccaggcca gggcaggaat gaatcgggca
     1501 acgtcaacaa tggtgagact attcgcgggg agtgacatct ggatgacgat gagttcttgg
     1561 ttgttgacct tgtggaaggc aaaggcccca aaagtcaatt gggcgttatg tcgcagtagc
     1621 atggcggcgt tctgctccga tgctggacca cataccgacc agaagcgaac aatgttttgt
     1681 cccccctctc gagattgttc gtagctgcac cagaccacct gcttgcgaag gttaccaacg
     1741 ggcacggtga ttttgagcgt gttgccgaat tcctccagga cataaccaga ctgacgcgcc
     1801 acggtcttca ccaattgcaa ggggtcgctc gcaccctcgg agggaaaccc cagcggtggc
     1861 ggcgcggctt gttggcccgc tttttccgcg aggggcaccg ccggaggcac ggc
//
LOCUS       DAOPTR010000832         1912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_47106_length_1912_cov_4.267636, whole genome shotgun sequence.
ACCESSION   DAOPTR010000832 DAOPTR010000000
VERSION     DAOPTR010000832.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47106_length_1912_cov_4.267636"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            378..1877
                     /locus_tag="PKI05_13630"
     CDS             378..1877
                     /locus_tag="PKI05_13630"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOA53289.1"
                     /translation="MNVILAATLLTNCLLAVGEAPSLPDTSKMNILLIDIEDCNAAAL
                     GCYGNPICKTPHLDRFVQTAVRFDRAYVQAVCCNPSRTSFLTGYRPLTTRVTSNGQEM
                     DAHLPEGMLTLPEYLKTRGLYLAVIGKLFHRTDYAEKQLSVFDRIEMYTPPPGWKGPG
                     PVIKFPPVARPAGVPKPPADEKSPEYREWRRQQSDRYGDSGRAPEEEGDYRMAATAVA
                     LLKEFAQTKQQFFLAVAQSKPHTPLLAPKKYIDMYNPADIPDPPAPAESLVNFPYPKR
                     AFGGNPDIFTLKQPTKQQSKEAIAAYYACLSMVDNHVGMILKALEETGLDTNTIVIVL
                     GDHGFHLGDHGFWSKYSMLEATHRAPLIVRVPGAPGNGQACDQIVEFVDLVPTIGDLL
                     KIDLPKDMEGISFAPLLADPNRPWKTAAFIVEDDGDAFGECVRTKKYSYMEFKKGAIP
                     AALFDLEKDPWETKNVVDDPAYAQVRKEMAELLHAGWKKALPPGYSTDK"
BASE COUNT          453 a          540 c          524 g          395 t
ORIGIN      
        1 ccacgccacg gacgcccacc gtacaccacg ggagacagaa gatttcatag gctcgtcctc
       61 tcggatattc tgcggatcac cccggcacgc cgccaatcat atcatagggc atgatcacct
      121 aaagaagcaa caagccaaac gaaaagagac gggttgcgaa acatgaaaaa ccgttaaccc
      181 tcgattcgga acccatgggt gcgatccaga gagtgccctg cggacaaggg tccggtatat
      241 cctgcaagcc gaaagtaatt cctctataat ggttgcgagc atgccgcgaa aacggcatgc
      301 ggtctagtgc cacttcggcg cagaagccgt gtggtttgtc ggtttaggtg gttttcctga
      361 tttggggagg tcctaccatg aatgtcattt tggccgcaac gctattgact aactgcctgt
      421 tggccgtcgg cgaggcgcca tcgctgcccg acacatcgaa gatgaacatt ttgctcatcg
      481 atatcgagga ttgcaatgcc gcggcgttgg ggtgctatgg taatcccatc tgcaaaacgc
      541 cgcatctgga tcggttcgtc caaacggctg tgcggttcga tcgagcttac gtccaggcgg
      601 tctgctgcaa tccgtcacgg acatcgtttc tcacaggtta tcgtccgttg accactcgtg
      661 taacaagcaa tggccaggaa atggacgccc atctgcccga aggaatgctg acgttgcccg
      721 agtatctgaa aactcgtggc ctttacttgg ccgtgatcgg caagttattt catcgcacgg
      781 attacgccga aaagcaactt tcggtgttcg atcgcatcga gatgtacaca cctccgccgg
      841 gctggaaagg ccccggtccg gtgatcaagt ttccacccgt agcgagaccc gccggcgttc
      901 ccaagccacc agccgatgaa aagagcccag aataccgcga gtggcgacgc cagcagtccg
      961 accgctacgg cgactcggga cgagcgcccg aagaggaggg ggattaccgt atggcggcca
     1021 cggccgtagc gctgctcaag gagttcgcgc agacaaagca gcaattcttc ttggcggtag
     1081 cccagtcgaa accccatacg ccattgcttg cgcccaaaaa gtatatcgac atgtacaatc
     1141 ctgccgatat ccccgatcct ccggctcctg cggaaagctt ggtgaatttc ccctatccca
     1201 agcgggcgtt tggcggcaat cccgacatct tcaccctaaa acagccgacc aaacagcaaa
     1261 gtaaggaggc catcgccgcg tactacgctt gtttgagcat ggtcgataat catgtcggca
     1321 tgatcctcaa agccctggaa gaaacgggcc tagatacaaa caccatcgtg atcgtcctag
     1381 gtgaccacgg atttcatctc ggcgaccatg gtttctggtc caaatactcg atgttggaag
     1441 caacgcatcg ggctccactg attgtgcggg tccccggcgc accaggcaat ggtcaggcct
     1501 gcgaccaaat cgtcgagttc gtcgatcttg tgcccaccat tggtgacctt ctgaagatcg
     1561 acctaccaaa ggacatggaa ggtatcagtt ttgcgccgct cttggccgat cccaatcgcc
     1621 catggaagac ggcggccttt attgtcgagg atgacggcga cgccttcggc gagtgtgtcc
     1681 gcacgaagaa gtatagttac atggagttta agaaaggcgc tattcccgct gcgctttttg
     1741 atctggagaa agacccctgg gagacaaaga acgtcgtgga tgatccggca tacgcccagg
     1801 tacgaaaaga gatggccgaa ttgcttcacg ccggttggaa gaaggcctta ccgccgggct
     1861 actcaacgga caagtagttc ccagcggatt gggagaagtc ttccgaagct gc
//
LOCUS       DAOPTR010000833         1910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_47185_length_1910_cov_2.906199, whole genome shotgun sequence.
ACCESSION   DAOPTR010000833 DAOPTR010000000
VERSION     DAOPTR010000833.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47185_length_1910_cov_2.906199"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PKI05_13635"
     CDS             <1..197
                     /locus_tag="PKI05_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53290.1"
                     /translation="KQFWSELVIFWQTLKPLRKLLAVRKIVRLLRPFGRALGSSSYLR
                     GSRPNGRPERRALRELSANQ"
     gene            421..918
                     /locus_tag="PKI05_13640"
     CDS             421..918
                     /locus_tag="PKI05_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53291.1"
                     /translation="MSMFQDSNYHWRETCFVYFSRENRPTLQQVIRTLRTVGPQYQVM
                     NAQADQAGRFESITVIAPDAFSAMDICYLDGPEIVEEVEKQVKDLSASDLTAEERKQL
                     ARLKTCNARLDVFHFERLMDDDEDDESDEPGDFFDPSALIIVLEAFCKLTRGIAIDPQ
                     SGTFL"
     gene            985..1557
                     /locus_tag="PKI05_13645"
     CDS             985..1557
                     /locus_tag="PKI05_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008684780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOA53292.1"
                     /translation="MLPAKDIKRGMIVSYQDAPCLIESVQVQTPSARGAATLYKFRAR
                     NLLSKQKVDFTLKRPEGLEEANFERRPVTFMYADADSVHFLDSVDYNEYVLPKEQLSD
                     ELNYLKEGLEGIFALIFEGACVGIQLPVAVELKIVQCDPYVRGASATGRTKPAVLETG
                     LVIQVPEYMSEGEIVKVDTRTGEFLSRVSS"
     gene            complement(1627..>1910)
                     /locus_tag="PKI05_13650"
     CDS             complement(1627..>1910)
                     /locus_tag="PKI05_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53293.1"
                     /translation="RPGPCYLIATAWRAQGDIERAMLAYLKPPILWPENRRIAARCLW
                     EVASLLAKDQTEFGGVARRLQTERLYQELATSYPESPWSRQIPKSQGTN"
BASE COUNT          444 a          480 c          543 g          443 t
ORIGIN      
        1 acaagcagtt ctggtcggag ctcgtcattt tctggcagac gctcaagccc cttcggaagc
       61 tcctcgcagt tcggaagatc gtcaggcttc tccggccgtt cgggcgggcg ttaggttcat
      121 cttcatatct tcgcgggtcc cgcccgaatg ggcgtcctga gagacgtgca ttgcgcgagc
      181 tgagcgcgaa tcagtgagat ggggttatcg tcgccacctt ctcccgtttc tcgcttgaaa
      241 gtgggggctt ttttcgcgaa tgcgttttcg acgattctcg acgcagatgg gtttggcagg
      301 gtagtcgcca gaacgtaaaa taagttttcg ctgtcagctg tgcctaggca cgggggaaca
      361 ggaccacttt tgacagtccg taaagcggac cgacataaaa ccgagggatg gaacaccgcc
      421 atgtctatgt ttcaggatag taattatcac tggcgggaaa cgtgttttgt ttacttctct
      481 cgcgaaaatc gcccgaccct acaacaggtc ataaggacct tgagaaccgt ggggcctcaa
      541 taccaggtta tgaatgcgca ggctgatcag gcagggcggt ttgagtcgat aactgtgatc
      601 gcgcccgatg ccttttcagc catggacatt tgctatctgg acggcccaga gattgtggaa
      661 gaagttgaaa aacaggtgaa agacctctcg gcctctgact tgacagcgga agaacggaaa
      721 caacttgcac gactgaagac gtgcaacgcc aggcttgatg tgtttcattt cgaacgatta
      781 atggacgacg atgaggacga tgaaagtgac gagccgggtg actttttcga tcccagtgca
      841 ctgatcatcg tccttgaggc cttttgcaag ttaacacgcg gaattgccat tgatccccaa
      901 tcgggcacct tcctgtgatt ggtaaaatcc tcgagaacag cgacggtccg gggaaattag
      961 gatacaaagg gagagatttt ttcgatgtta ccggcaaagg atatcaaacg tggaatgatc
     1021 gtttcgtatc aggacgcacc ctgtttgatt gagtctgtcc aagttcagac gcccagcgcg
     1081 cgaggtgccg ccacgcttta caagtttcgt gcccgcaatc tgctgtcaaa acaaaaggtc
     1141 gattttacgc ttaaacggcc agaggggctg gaagaggcca actttgaacg ccgccccgta
     1201 accttcatgt acgcggatgc agattcggtc cattttctcg actcggtgga ctacaacgag
     1261 tacgtcttgc cgaaggagca actcagcgac gagctgaatt atctcaaaga gggactggaa
     1321 ggcatttttg cccttatttt tgagggagca tgcgttggga ttcaactgcc ggtggctgtt
     1381 gagctgaaga ttgtccagtg cgatccttac gtccgaggtg cgtccgccac aggtcgcacg
     1441 aagccagctg ttctggaaac cgggttggtc attcaggttc cagagtacat gtcggaaggc
     1501 gaaatcgtca aagtcgatac gcggacgggg gagttcctct ctcgggtgtc atcgtgaact
     1561 aaacaggcac ggcaagcagt gacaaacgtc agaaggctct gaacctatcc ctgcggaaaa
     1621 ggtcgctcaa ttggttcctt gggacttcgg gatttgacgg gaccacggtg actcgggata
     1681 gctagttgcc aattcttggt agagcctctc agtctgtaac cggcgtgcaa cgcccccgaa
     1741 ctccgtttga tccttcgcca aaagggacgc tacctcccaa aggcatcgcg cagcgatacg
     1801 ccgattttcc ggccagagga tcggtggttt aagataagcc agcattgccc gctcaatgtc
     1861 cccctgcgct cgccaggctg tggcgataag ataacagggg ccaggccgaa
//
LOCUS       DAOPTR010000834         1909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_47210_length_1909_cov_3.049622, whole genome shotgun sequence.
ACCESSION   DAOPTR010000834 DAOPTR010000000
VERSION     DAOPTR010000834.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47210_length_1909_cov_3.049622"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /gene="rho"
                     /locus_tag="PKI05_13655"
     CDS             <1..1013
                     /gene="rho"
                     /locus_tag="PKI05_13655"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOA53294.1"
                     /translation="SQIRRFGLRTGMTVSGQIRPPKENERYFALLRVEAVNYEDPNLA
                     AKKIWFDDLTPCHPDKRIILETTPDEIECRVVDLIAPVGFGQRGLIVSPPRAGKTILL
                     QKMAKAVLKNYPDCYVFMLLIDERPEEVTDMERQVKGRNCEVISSTFDEAPARHIQVS
                     EMVIEKAKRFVEYGKDVIIFLDSITRLARAWNAECPASGKILTGGVDANALQRPKRFF
                     GSARKIDEGGSLTIIATALIDTGSKMDEVIFEEFKGTGNMEIVLDRRLVDKRIWPAID
                     INRSGTRREEMLLDPEEHRRICVLRRVLNEMNPPDAMEFLVSRLAKTKTNAEFLMSMN
                     MR"
     gene            1108..1707
                     /gene="ribH"
                     /locus_tag="PKI05_13660"
     CDS             1108..1707
                     /gene="ribH"
                     /locus_tag="PKI05_13660"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOA53295.1"
                     /translation="MESAVGLLPVGRIFAGDKSLPSGRFAIVVSRFNETITEGLLRGA
                     VETLTAAGVDPEQIDVFWVPGAFEIPTVAKRLALSGRFLAILCLGAVIKGETTHDQHI
                     NRAVSLQLAEIGVQTGVPVLFGILTCNTFEQALARSASREIIRDKDASQKKVGNKGAE
                     CALAALEMVRLMHQIDHTPDLAIPTTPDAGRIASERVEG"
     gene            1721..>1909
                     /locus_tag="PKI05_13665"
     CDS             1721..>1909
                     /locus_tag="PKI05_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53296.1"
                     /translation="MTRTIPDRRNARIVVVQILYQDDVNPEHNPAWDEEWIKRRLRHP
                     ELVAFAKSLLAGVRLYQRE"
BASE COUNT          445 a          507 c          545 g          412 t
ORIGIN      
        1 ccagtcaaat tcgacgtttt ggcttgcgga cggggatgac ggtttcggga cagattcggc
       61 ctcccaaaga aaatgagcgg tactttgccc ttctccgggt ggaggccgtg aactacgaag
      121 acccaaactt ggccgctaaa aaaatctggt ttgatgactt gacgccctgt caccctgaca
      181 aacgcatcat tttggagacc acccccgacg aaatcgaatg ccgcgttgtc gacttgattg
      241 cccccgttgg gttcggacag aggggcctga tcgtcagccc gccacgggcc ggaaagacca
      301 tcttgctcca aaagatggcc aaggcggttc tcaaaaacta cccggactgc tacgtgttta
      361 tgctcctgat cgacgagcga ccggaagagg tcaccgatat ggagcggcag gtcaagggcc
      421 gcaattgcga ggtgattagc tcgacatttg acgaggcccc cgcccgccat attcaagttt
      481 cagagatggt catcgaaaag gccaagcgat tcgtcgaata cggcaaggat gtaatcatct
      541 tcctagattc gatcacccgt ttggcgcggg catggaatgc cgaatgtccg gcgtcgggaa
      601 aaattttgac aggcggtgtc gatgccaacg ccttgcaacg gcctaagaga ttcttcggtt
      661 cggcccggaa gatcgatgag ggcggttccc tgacgatcat cgccacggcc ctgatcgaca
      721 ccggtagcaa gatggatgag gtgatttttg aggaattcaa agggaccggt aacatggaga
      781 tcgtcctcga tcgccgactt gtcgataagc gaatttggcc ggcgatcgac atcaatcggt
      841 cgggcacacg ccgcgaggag atgttacttg accccgaaga gcaccgccga atttgtgtct
      901 tgcgacgggt cctcaacgaa atgaatccgc cagacgccat ggagttcctc gtcagccgtc
      961 tggccaagac taagacgaac gccgagttcc tcatgtcgat gaacatgcgt taattgtttg
     1021 gcaggctggc gggtcttgac gcccaacctg gacgaggctc ctggctgata aacgccacat
     1081 tatctgaaag ggacacggga gtattccatg gaatctgctg ttggactgct gccggtagga
     1141 cgcatctttg ccggggacaa aagcctaccg tctggccggt ttgccattgt tgtctcccgc
     1201 tttaacgaga cgatcacaga ggggcttttg cgaggggcag ttgaaacgtt aacagcagca
     1261 ggtgttgatc ccgagcagat cgacgttttt tgggtccccg gcgcctttga aattcccacg
     1321 gtagccaaac ggttggcact gtctggccgt tttttggcga tcctctgtct gggagctgtc
     1381 atcaaaggtg aaacgactca tgatcagcac atcaatcggg ccgtcagttt gcaactggcg
     1441 gagattggcg tccagacggg tgtcccggtc ttgtttggaa tcttgacgtg caatacgttc
     1501 gagcaggccc tcgcccgatc ggcctcccga gaaatcatcc gagacaaaga tgcctcccaa
     1561 aagaaagtcg gaaataaagg ggcagagtgt gccctcgctg ccttggaaat ggtgcggcta
     1621 atgcatcaaa tcgaccatac ccccgatctt gcaatcccta cgactcctga cgctggccga
     1681 atcgcctccg aaagagtgga aggatgatgt gagcccgcca atgacacgca ctataccgga
     1741 tcgaagaaat gcccgaattg tcgtggtcca aattctctac caggatgatg taaaccccga
     1801 acacaatccg gcttgggatg aggagtggat aaagaggcgg ctgcggcatc ccgaactggt
     1861 ggcctttgcc aaatcgctac tcgctggcgt tcggctttat caacgtgag
//
LOCUS       DAOPTR010000835         1905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_47331_length_1905_cov_3.228649, whole genome shotgun sequence.
ACCESSION   DAOPTR010000835 DAOPTR010000000
VERSION     DAOPTR010000835.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47331_length_1905_cov_3.228649"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PKI05_13670"
     CDS             <1..525
                     /locus_tag="PKI05_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOA53297.1"
                     /translation="KGAAAEEIAMLFDTARRVVIVPGYGMAVAQAQHAVRDLYQLLES
                     RGIEVLFGIHPVAGRMPGHMNVLLAEAEIPYDKLKELDEINSILSETDVAIVLGANDV
                     VNPLAKTDPKSPIAGMPIIEVDKAKTVVVIKRSLSPGFANIPNPLFAADNTLMFFADG
                     KRAVLDLIAAIKES"
     gene            624..1652
                     /locus_tag="PKI05_13675"
     CDS             624..1652
                     /locus_tag="PKI05_13675"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOA53298.1"
                     /translation="MRQPLQNCLFCAFLLVFMGNAPLMAESHTVLGVEGSRFTVNGQP
                     TFLLGVSYYGALGVGPEVWKSDLKEIKKRGFNWVRIWANWNAFGQEAFAVDAEGNPYD
                     AGLKRLQAIVAYCDELGLIVDVTLSRGNGVTGPKRLQTLDAHLRAVRVLVTALKDYRN
                     WYLDLSNERNIRDARFTSIEDLVILRKEVRTLDPKRLVTASHAGDISEEELRDYVLKV
                     GVDFISPHRPRRAGSPQETEAKTREYLRQMETIGRVVPVHYQEPFRRAFGWQPTAEDF
                     LIDLKGAYRGGAAGWCFHNGDTRNAHDGRPRRSFDLRDGNLFTQLDSEEKRAIELIAQ
                     FAKDVLTK"
BASE COUNT          446 a          465 c          537 g          457 t
ORIGIN      
        1 aagggcgcgg cggcagaaga gatcgcaatg ctttttgaca cggcccgacg tgtggtcatt
       61 gtgccgggtt acggcatggc agttgcacag gcacaacacg ccgtacggga tctttatcaa
      121 ttgttggaat ctcgcggaat tgaagtgctt tttggtattc acccggttgc cggacgtatg
      181 cccggccaca tgaatgtgtt actagcggaa gccgagattc cctacgacaa gcttaaggaa
      241 ctcgacgaga tcaattccat tctctcggaa accgatgtgg cgattgttct cggggcgaac
      301 gatgtggtaa atccgctggc caagacggat cccaaaagcc ccatcgcggg aatgccaatc
      361 attgaggtag ataaggctaa gacggtggtc gtgatcaagc ggagcctcag cccgggattt
      421 gccaatatcc ccaatccact tttcgccgct gataatacgc tcatgttttt tgcggatggc
      481 aagcgggccg tccttgacct gattgcagcg atcaaagaaa gttgataaaa cctgacggca
      541 gacgtcctcg acagtcctgt tcgcaagacg tgtcgatttt gaatcagctt cgagttggga
      601 gcagcagtag gggaggccat gccatgcggc aaccattgca gaattgcctg ttttgcgcat
      661 ttttgcttgt cttcatggga aatgcgccgt tgatggcaga gagccacacg gttcttgggg
      721 ttgagggctc acgttttacc gtcaatggcc agccaacttt tcttttgggc gtaagttact
      781 atggcgcttt gggggtcggc cctgaggtat ggaaatccga cctgaaagaa ataaagaaac
      841 gcggcttcaa ctgggtccgc atttgggcca actggaatgc cttcggtcaa gaagcatttg
      901 cggttgatgc ggagggaaac ccttatgatg cgggtctcaa acggctccag gctattgtcg
      961 catattgcga cgagcttggc cttatcgtag atgtaacgtt gtcacgaggc aacggggtga
     1021 cgggccccaa gcgacttcag actctcgatg cgcatctgcg ggcggtacgc gtactggtga
     1081 ccgcgctgaa agactaccga aactggtacc tggatctctc caacgaacga aacatccgcg
     1141 atgcacgctt cacgtcgatc gaggacttgg tcattttgcg aaaggaagtt cgtacgctcg
     1201 atccaaaacg gttggtgacg gcctctcatg ctggggatat ttcggaggaa gagttgcgag
     1261 actacgtgct gaaagtggga gtagacttca ttagcccaca tcgtccccgt cgggcgggtt
     1321 ctccacagga gacggaagca aagacccgtg aatacttgag gcaaatggag accataggaa
     1381 gggttgtccc agtccactat caagagccgt ttcgcagggc ctttggttgg caaccgacgg
     1441 cagaggactt tctgatcgat ctcaagggcg cctaccgagg gggagcggct gggtggtgct
     1501 tccataacgg cgatacccgg aacgcccacg atgggcgacc acgaaggtca ttcgatctga
     1561 gagacggcaa cctatttacg caattggatt ctgaagaaaa gcgggcaatt gaattgatcg
     1621 cgcagtttgc gaaggatgtc cttacaaaat gagcggtatt ctctcctgac cagcaagttc
     1681 attcatttct gtatccttct tgtccgtcaa tcttcttttg tcgtttactt attttttcca
     1741 atttggagtg actctcgagg ggcggcattt cttatcctat cgcgagcctt gcgggaacgg
     1801 gttccctttc tacccagctg acgaggtcta aaaccgcacg accgatttct ttctcatctc
     1861 acgaaaaacc tgcaagttca gagaagttta acacgataaa agagg
//
LOCUS       DAOPTR010000836         1905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_47335_length_1905_cov_2.983784, whole genome shotgun sequence.
ACCESSION   DAOPTR010000836 DAOPTR010000000
VERSION     DAOPTR010000836.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47335_length_1905_cov_2.983784"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PKI05_13680"
     CDS             complement(<1..272)
                     /locus_tag="PKI05_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53299.1"
                     /translation="MPTQWGAFLATASLAEQTPLPEETPKKETRLEGPRDVLAAFGVT
                     ESFLQSFEDGQPVQPAERELLLRVLFYSHMFQPPEIAEWAESPPCWS"
     gene            complement(438..1301)
                     /locus_tag="PKI05_13685"
     CDS             complement(438..1301)
                     /locus_tag="PKI05_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53300.1"
                     /translation="MLRFDGYRRAPSLPSQREQRRLLLLVLLIGGVILIPQWLISWRL
                     FAPDTFLNNERHNSRSRVAQPETLSRSQDDSRFAGRSEELLKQLSEIDFSAVEDNTPF
                     GKKETTAWFSTLHLLTQFSVDDLRSANPPWVTFSQLYRNPSNYRRKLVALRGEFRGIA
                     YYPAPANDKGIDGYYQAWLQPERENDPVVVYCLQLPPGFPEGMRIQEPVEVIGVFFKR
                     WVYQAQDGLRVAPVVLTKEPMWKRTPISTTGSLTSPQNILLAVAISVVLTVILLAYLF
                     RPHQRHIPELS"
     gene            complement(1428..>1905)
                     /locus_tag="PKI05_13690"
     CDS             complement(1428..>1905)
                     /locus_tag="PKI05_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53301.1"
                     /translation="FQRLAQHLIDGSGLGQTGWNNQGCPFYGHGVGYTGHFQWKKGEG
                     QYLVRLGRWEGSLAKVILNGRDCGVIFKPPYECDIASALKEGDNTVTVVVIGTLKNTL
                     GPHHAGVMRGSAWPHAFAQGPAEGPPAGDRYDTIAYGLFEPFRVVHRVPSSNFGAR"
BASE COUNT          471 a          515 c          513 g          406 t
ORIGIN      
        1 gaccagcagg gtggtgactc ggcccactcg gcaatctccg gcggctgaaa catgtgcgaa
       61 taaaagagca ctcgcaaaag aagctccctt tctgctggct gtacgggctg cccatcttca
      121 aaactttgga gaaagctctc ggtaacacca aaggcggcca aaacgtcccg aggcccttcc
      181 aagcgtgttt ccttttttgg tgtctcctcg gggagaggtg tttgctccgc caaactggcc
      241 gtcgcgagga atgcccccca ctgagtgggc atcgcttggg ccgccaggcc acacgtttcg
      301 caacgcagcc aagataggcc caataggcca agcagtacga gccaacacgg tattgatctt
      361 ttcactaaaa gagcctttcc ataccggcaa gctttcggag acatgttcat aaaatttatc
      421 cgccaagtac gtggaggtta cgacaactcg ggaatatgtc tttggtgagg tctaaaaaga
      481 taagccaaaa ggatgacggt cagaacgact gagatggcaa cggccagaag gatattctgg
      541 ggtgaagtca aagatcccgt cgttgagata ggagtgcgtt tccacatcgg ctcctttgtc
      601 agaacaactg gcgccacccg caatccatct tgggcctgat aaacccaacg cttgaaaaaa
      661 acgccgatca cttcgaccgg ttcctggata cgcattccct caggaaaacc agggggcaat
      721 tgcaggcagt aaaccaccac agggtcattt tcccgttccg gttgcaacca ggcctgataa
      781 tatccgtcga tccccttgtc gttagccggt gccggatagt aggcaatgcc cctaaactca
      841 ccgcggagag cgacaagttt tcttcggtag tttgagggat tccgatagag ttgcgaaaac
      901 gtgacccatg gtgggttcgc agaccggaga tcgtccacag aaaattgggt gagaagatgc
      961 aaggtagaaa accaggcggt ggtttctttt ttgccaaagg gtgtgttatc ctcgaccgcg
     1021 gaaaaatcga tctcggacaa ctgcttgagg agttcttcac tccgtccagc gaaccgcgaa
     1081 tcatcctggg accttgaaag agtttccggt tgagcaacac gcgatcgaga attatgtcgt
     1141 tcgttgttca agaaggtgtc cggagcgaaa agtctccagg aaatcagcca ctgggggatc
     1201 aaaataaccc caccgattag cagcaccagg agcagaagcc gcctctgctc ccgctgcgaa
     1261 gggagagacg gtgctcgccg gtagccatca aagcgaagca cggtgaaaac tcctatcgaa
     1321 tctgcaaaaa tcgcccatgt gccgggcacg cgcggcatag atcagcttgt ccagggcatc
     1381 cttccctcat tctaggttac ttctcctaga gggcaaaaga tgtgaaatta ccgtgctccg
     1441 aagttggaac ttggcacacg atgaaccact cggaacggct cgaataatcc atacgcgatg
     1501 gtatcgtagc gatcgcccgc gggtgggcct tcggcagggc cttgagcaaa agcgtggggc
     1561 caggctgagc cgcgcatgac tcccgcatga tggggtccca gagtattctt gagcgtgcca
     1621 ataacgacga ctgtgacggt gttatcaccc tctttcagtg ccgacgcgat atcgcactcg
     1681 taagggggtt taaagataac gccgcaatcc cggccattga ggatcacctt ggctagtgat
     1741 ccctcccagc gacccaatcg cacaaggtat tggccctctc cttttttcca ctgaaagtgc
     1801 cctgtgtaac cgacgccgtg gccatagaac gggcatccct ggttattcca gccggtctgg
     1861 cccaatccac ttccatcgat gaggtgttgg gcaagtcttt ggaaa
//
LOCUS       DAOPTR010000837         1903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_47409_length_1903_cov_3.402056, whole genome shotgun sequence.
ACCESSION   DAOPTR010000837 DAOPTR010000000
VERSION     DAOPTR010000837.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47409_length_1903_cov_3.402056"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..633)
                     /gene="dtd"
                     /locus_tag="PKI05_13695"
     CDS             complement(160..633)
                     /gene="dtd"
                     /locus_tag="PKI05_13695"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOA53302.1"
                     /translation="MKACIQRVRWARVRVNGETVGEIGPGLLVLLGVAMDDTEQEARM
                     LAEKIVGLRIFEDEAGKMNRSVGDAGGSLLVVSQFTLLADCRRGRRPSFTDAAPPDRA
                     NMLCEYFIQQVRKTGLPVATGRFREHMEVELLNDGPVTIVLDSTELKSPRREHGG"
     gene            874..>1903
                     /gene="pdxA"
                     /locus_tag="PKI05_13700"
     CDS             874..>1903
                     /gene="pdxA"
                     /locus_tag="PKI05_13700"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOA53303.1"
                     /translation="MSAGNDRPLIAITTGDPAGIGPEIIVAAWSDPRVHGICRPVVVG
                     YSEYLRRAAALRRSNVQIETCDDPQKARPSPNVIFCVAPGEDRAPPVQVGRIDPRAGQ
                     AAFEAILLAAELALAKKVDGLVTAPINKEALHAAGHQYPGHTELLADLCQRQQVAMVL
                     YLGPGAPLRGTAGLAIAHATLHIPMREVIHQLTAEAVFTKIRLIDQLMRCIKGSEPRI
                     GVCALNPHAGEGGLFGREESLVITPAIKRATDEGIRVEGPVPADAIMVQARDGRFDGL
                     VAMYHDQGHIALKLLGMHRAVNITAGLPIIRTSVAHGTAFDIAGLGRADPSSLIEAIR
                     VAAMLSKNR"
BASE COUNT          421 a          545 c          514 g          423 t
ORIGIN      
        1 atcccgtaca caatcgagaa tagcacgacg gcacccataa ccaggtcata gatcggtatg
       61 ccgagatcaa ccacggttct tcaagacctc caccttgacc gtatctgctg cggctttccg
      121 tgcattctgt aattctttag caaccccacc gacgaaccat tacccgccgt gttcgcgtcg
      181 cggcgatttc aactcggtgc tgtcaagcac aatggtaacc ggcccatcat tgaggagctc
      241 gacctccatg tgttcccgaa accgccctgt ggctaccggc aaacccgttt tgcgaacctg
      301 ttgaatgaaa tattcgcaaa gcatattcgc gcgatctggg ggagctgcat ccgtgaagct
      361 gggacggcgc ccgcgacgac aatcggccag caaggtaaac tggctcacga ccaagaggga
      421 tccgccggca tctcccaccg agcgattcat ctttcccgcc tcgtcttcga atattctcaa
      481 tccgacaatc ttctcggcga gcatccgtgc ctcttgttcg gtatcgtcca tggccacgcc
      541 gaggagtacc agcaatccgg ggccgatctc gccgacggtt tccccgttta cacggaccct
      601 tgcccagcga acacgttgaa tgcatgcttt catgcgaagg tgctcaatag atttgacttc
      661 tccaaaccaa gcctggtttt ttgatggcat atctgcgcga tctgccttta atatcataga
      721 atgtctctcg aaggggcgga aacaccgatt ttgtggcaga aagtatttgc tcaacggtga
      781 agggcgacct taaaatagcg ggcaaagagg tccctgatca ccgagagggt acaaaaatca
      841 tttttctcct gcccgagaca gaggataaca accatgagcg ccggaaatga tcgtcctcta
      901 atcgccatta caaccgggga ccctgcggga attggtccag aaatcattgt ggccgcttgg
      961 tctgatcccc gcgtgcacgg tatctgtcgc cctgtcgtgg ttggctattc ggagtatctc
     1021 cggcgggctg ccgccctgcg acgttccaac gtacaaatcg aaacctgcga cgatcctcaa
     1081 aaggccagac cctcaccgaa tgtcattttt tgtgtagctc ctggggaaga ccgagcacca
     1141 cccgtgcagg tgggccgcat cgacccacgc gctggccagg cagcctttga ggccatcctg
     1201 ctggctgccg aattggccct ggccaagaag gtggatgggc ttgttacggc accgattaat
     1261 aaagaggccc tccatgcagc tggccatcag tacccgggac acacggaact gcttgccgat
     1321 ttatgtcaaa gacaacaagt ggcaatggtt ctgtatttgg gaccgggcgc gcccttgcgg
     1381 gggacggcag ggttagccat tgctcacgcg acgctccata ttccaatgcg ggaagtgatt
     1441 caccaactaa ctgcggaggc cgttttcact aagatacggc tcatcgatca gctcatgcgg
     1501 tgtatcaaag ggagtgagcc ccgtattggt gtctgcgccc taaaccctca tgcgggggag
     1561 ggaggcttgt tcggcaggga ggaatctttg gtgattacgc cggccatcaa acgggcaacc
     1621 gacgaaggaa ttcgggtgga gggaccggta cccgccgacg ctattatggt acaagcccgc
     1681 gatggtcgat tcgacggttt ggtcgccatg tatcatgatc agggacatat cgcccttaag
     1741 ctccttggga tgcaccgcgc tgtcaatatt acagcaggcc ttccgatcat acgaacgagt
     1801 gtggctcacg gtacggcttt tgatattgcc ggccttggtc gggcagaccc gagcagtctc
     1861 atcgaggcga ttcgtgtggc cgctatgctt tccaaaaatc gtc
//
LOCUS       DAOPTR010000838         1903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_47415_length_1903_cov_3.012987, whole genome shotgun sequence.
ACCESSION   DAOPTR010000838 DAOPTR010000000
VERSION     DAOPTR010000838.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47415_length_1903_cov_3.012987"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PKI05_13705"
     CDS             complement(<1..351)
                     /locus_tag="PKI05_13705"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOA53304.1"
                     /translation="MMDYLQILLLAIVQGLAEFLPISSSGHVMVLAHLCDLSGTPLPD
                     KLAVNVILHFGTLLAVLVYYRGRIVAMIRHDWKTIGLLAVASLPAGFIGVGYKLWLED
                     WLTSQMGTNLLEHPL"
     gene            complement(1156..>1903)
                     /locus_tag="PKI05_13710"
     CDS             complement(1156..>1903)
                     /locus_tag="PKI05_13710"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOA53305.1"
                     /translation="SLQIVGVQIDFSPSQLLSVIQHVAQTNHQLLDPRDDLGVSVFVT
                     PGRYIGYSGPGPVVPTVCVHTYPLPFFLWAKKYRTGESAAISTVRQVPTSCWPRELKG
                     RSRMHYYLAEQEVQKRYPGSRAILLDQDDSVTESSIANVVMCDRDGRLLAPPLERTLP
                     GISLKVVTELASKLGIPFEFCPLTVAYLETATEIFFTSTPYCVLPVTSFNGRPVGNGQ
                     PGTVFAKILAAWNELTGVDIVAQAELFSDR"
BASE COUNT          493 a          502 c          503 g          405 t
ORIGIN      
        1 cagggggtgt tcgagaagat tcgttcccat ctgactcgtc aaccagtcct cgagccagag
       61 cttataaccg accccaataa atcccgccgg gagactggcc accgccagca aaccgatggt
      121 cttccaatcg tgacgaatca tcgcaacgat gcggccccga taatagacca gcacggccaa
      181 cagggtccca aaatggagaa tgacgttgac ggccagtttg tcaggcaaag gggttccaga
      241 tagatcgcac agatgggcga ggaccatcac gtgcccggaa gagctgatgg gtaggaactc
      301 cgccaaccct tggacaattg ccaaaagaag aatttggagg tagtccatca tttggaaaat
      361 gcgaccctgg taagaatgcc tcgggcgaag aagactactc ttgcggacag aatctccaag
      421 aaaaaaaagt gcccttcgta ggcggggcag ggacctacga agggctgcgc tgtgagggtc
      481 ccctcacgca cttgtattat ttcacgttct tcgtccattt gcaagtccct gttgagaaca
      541 atccgttgag agatttgact aatgcacaag aatatcattt tctggcgccg tttcatcgat
      601 gattgtgtca cagaagtgaa catcttccat cgcttttttg aaaaatactt cgtctttatg
      661 ggcaccgctg atgggttttt gggaccatct atcttatggt aaacgcaggc gcggtcacgc
      721 ctcccagcct cacctctcga ctgcacgagc agcctgaacc gtcttcattt cacaagacaa
      781 accgcccaac ggtcaagcac ttgcctgacg gcttcaacaa tggcgcctta gcgaccaaac
      841 gtatgtgtat tgcccacggt accagtttgt tttgcgcaag tgtgcgaacc aaactgcaat
      901 gacgctcggg aaaacctgtt cggggagacc tcagacaaac atacaccaga aagaggtcgg
      961 aaccaaatta tcttaaggaa attgtcgcag aaaatatgtg gccgtttgtt acctcattgc
     1021 gcctcgtctt gtcgcacacg tcgtcttttt ccgaagaggt gaccgaatcc ttatagggca
     1081 atccctcacc ggagtgagag tgactcatcg ggtggtgatt cccgtcagcc gggaacggtg
     1141 ggatcgcaat caaactcaac ggtcagaaaa caattcggct tgggctacga tatccacgcc
     1201 cgttagttcg ttccacgccg ctagaatctt cgcgaaaacc gtcccgggtt gaccattccc
     1261 gacgggccga ccattgaacg acgtcaccgg gagtacacag tacggtgtgc tggtgaaaaa
     1321 aatctctgtt gcggtttcca aataagcgac ggtcaaggga caaaactcaa agggaattcc
     1381 cagcttgctg gccaactcgg tgacgacctt aaggctgatg cccggaagcg tccgctcgag
     1441 cggcggcgcg agtaaacggc catcgcgatc gcacatgacc acattggcaa ttgaagactc
     1501 ggtgaccgag tcgtcctgat ccaaaagaat ggcccgactt cccgggtagc gtttttggac
     1561 ctcctgttca gccagataat aatgcatccg actgcggccc ttcagttccc gtggccagca
     1621 ggacgtggga acttggcgca cagtggaaat ggccgccgac tccccagtac ggtatttctt
     1681 ggcccaaaga aaaaagggaa gcggataggt atgaacacat acagttggaa cgacaggtcc
     1741 tggtcccgag tatccaatat atcggcccgg cgtcacaaac acagaaaccc cgaggtcatc
     1801 acgagggtca agaagttgat gattcgtttg cgcgacgtgc tggatgacgg agagcaattg
     1861 ggagggtgag aagtcaattt ggacccccac gatttgtaga gag
//
LOCUS       DAOPTR010000839         1897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_47621_length_1897_cov_2.689468, whole genome shotgun sequence.
ACCESSION   DAOPTR010000839 DAOPTR010000000
VERSION     DAOPTR010000839.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47621_length_1897_cov_2.689468"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PKI05_13715"
     CDS             <1..180
                     /locus_tag="PKI05_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53306.1"
                     /translation="TDVANPGKVPPRNWMNLRPLGNVLQIVDLLGPCSVSFLRVSARA
                     MGYRSSDFRGTGGAL"
     gene            239..694
                     /locus_tag="PKI05_13720"
     CDS             239..694
                     /locus_tag="PKI05_13720"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53307.1"
                     /translation="METAKQISVKLVNKPGRLAQLLDAMAKAKVRCRALVAMDSGDRG
                     TVRFVPTEWERAKRVLEELNLPYELADVLLVDVPKQPGGFRKICERLAEHHLNIDYAY
                     HSFGSELGAKGGGVAVIKVNDLNKAQRVLSTNDSAGKQRASVRRPVYAR"
     gene            933..1676
                     /locus_tag="PKI05_13725"
     CDS             933..1676
                     /locus_tag="PKI05_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53308.1"
                     /translation="MPPTALAMGFRLNCLWGCLIWSTLAVVPAGCFRPWFLSPPPVLS
                     CYYPNPIFVRAKNPEQFWEALVDIIDDDFEIESESPVRQLQTVFTSGRIDTRPKVAAT
                     ILEPWHHDSPDRYSRWEATLQSLRRRAEVRVDPRDGGYWITVLVFKELEDVNQPLAAT
                     TSSATFRNDSSLTRVISPIGEQQVNAGWIPLGRDTALEQRLIEKIAARFGAALHHLLG
                     RHGQYLHQPPGAGATAQVLHLARRRQDDS"
BASE COUNT          363 a          563 c          571 g          400 t
ORIGIN      
        1 actgacgttg ccaaccccgg aaaagttccc cctcgcaatt ggatgaacct ccgtccgctg
       61 gggaatgtcc ttcagattgt tgacttgctt gggccttgta gcgtatcctt tttacgggtg
      121 tccgcccgcg ccatgggata tcgatcaagt gatttccgtg gcacgggagg ggccctgtaa
      181 gccttctccg tgaggggtgt tgttggccaa cccggttttt caacgaaagg gagaaacgat
      241 ggaaacggcc aagcagatct cagtgaagct cgtcaacaag cctggacgtc tcgcccaatt
      301 gctcgatgcc atggccaagg ccaaggtccg gtgtcgtgct ttggtggcca tggactcggg
      361 tgaccgggga accgtgcgat tcgttcccac agaatgggaa cgtgccaaga gggtgcttga
      421 ggagcttaac ttgccctacg aattggcgga cgtcttgctc gtggatgtgc ccaagcagcc
      481 agggggattc cgcaagatct gcgagcgatt ggccgaacac catctcaata tcgactatgc
      541 ttaccattcg tttggcagtg agcttggtgc caagggtggg ggtgtcgctg tgataaaggt
      601 gaatgacctc aacaaggccc agcgagtgct ttcgacgaac gattccgcgg gcaagcagag
      661 ggcctcggtc cgacgccccg tttacgcccg ataagatacc ccgatcgaat tgtgcacccc
      721 ccctagtggg gtatctgcac cttgagttag tccatcgggc caagaggcgc cgcgatggcc
      781 cgacatggcg ggggggagtc tagcgctcgt tcgccgccga tttggggaga aaaagacccc
      841 tcttcggcac cagcgccgaa gtggccttgt ctattttgcg gcgtttgggg acacttcgcc
      901 agtcatcgga gagaaaggat tgcacacgag gagtgccacc gacggcgctg gctatggggt
      961 ttcgcttgaa ttgtctctgg ggatgtttga tctggagcac gctcgctgtt gtgcctgcgg
     1021 ggtgcttccg gccttggttc ttatcgcccc cgccggtcct cagttgctat taccctaatc
     1081 ccatctttgt ccgcgcaaag aaccccgagc agttctggga ggctttggtg gacatcattg
     1141 atgacgattt cgagatcgaa agcgagtcac cggttcggca attgcagacg gtttttactt
     1201 ccggccggat cgacactcgt cccaaagtcg cggctaccat cttagagccc tggcatcacg
     1261 attcgccaga ccgctattcg cggtgggagg ccactctgca aagccttcgc cgccgcgcgg
     1321 aggtccgggt ggatccccgg gatgggggtt actggatcac cgtcctagtc tttaaggagc
     1381 tcgaagacgt gaatcaaccc ttggcggcta cgacgagctc ggccacattt cgcaacgaca
     1441 gttctttgac gcgggtcatc agcccgatcg gtgagcagca agtcaacgcg gggtggattc
     1501 ccttgggcag ggacaccgct cttgaacaac gactcatcga aaaaattgct gcccgattcg
     1561 gtgcagcact acaccatctt ctcggccggc acggccagta cctccaccaa cccccagggg
     1621 ctggagcaac tgctcaagta ctacacctcg cccgccgccg ccaagacgat tcgtgacacc
     1681 ggcctggagc ccattgccaa gtcccgctga gcgcagcacg gccccgcaaa aaagcccgcc
     1741 aggcatacgc ctgcgggctt ttttgtcgcc tggcagctca gcgcttgctg cccgggccgg
     1801 ctttctgcac cagggccacg gtcagtccct gggcggtgac ggtgatggcg ccaggctcca
     1861 tggccagcgc atcgaccagc gccagctcct ggtcctg
//
LOCUS       DAOPTR010000840         1891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_47823_length_1891_cov_3.307734, whole genome shotgun sequence.
ACCESSION   DAOPTR010000840 DAOPTR010000000
VERSION     DAOPTR010000840.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47823_length_1891_cov_3.307734"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PKI05_13730"
     CDS             <1..572
                     /locus_tag="PKI05_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007335194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HOA53309.1"
                     /translation="SSHKADGKTWLDVHRTAHSLGLKSNATMLFGHVETIYHRVDHLL
                     ELRTLQDETGGFQAFVALPFHPVRTAFSHLPKPSAFDCLRTIAVSRLLLDNFDHIKAY
                     WVALGIGVAQTALEYGANDLDGTVRYENIYHDAGADSPDVLSVEELCALIRETGREPV
                     ERDSLYRRVRRFGREWELEDNHPDVRNRE"
     gene            585..>1891
                     /locus_tag="PKI05_13735"
     CDS             585..>1891
                     /locus_tag="PKI05_13735"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOA53310.1"
                     /translation="MTPLLGTLLGCAKPVRIMMNEGTKPSTISPRDQDPLRLPIQFVK
                     GVGPRRAEILAKLGIETVSDALFFFPRSYEDCSRVRPVNQLEEGTVQAVVGVVEDVES
                     RVLRGGQTVVGVLIAGEGGYVRAVWFQQPYRRDYFREGQRVMAVGKPRRNGLIWEMVH
                     PRVIVLGEEEQPKLAPLPVYALTEGIQQWEMRKMIGRVVAQYSGYLEEVLPEKILAQQ
                     RLLPIQEAVQQIHAPSDQQQIQAARRRFIYEELFILQVALALKRYQQITSKKSPILRL
                     TPKIDERIRRLFPFRLTAAQDQAVAEIVRDMEQPIPMNRLLQGDVGSGKTVVAVYAML
                     LAVANQHQAAMMAPTEILARQHFQTINQLLEKSRVRRTLLVGGLPPQERSQILRQIAE
                     GEIDLVVGTQAIIQEEVEFHRLGLVVIDEQHKFGVRQRAALKGA"
BASE COUNT          429 a          473 c          552 g          437 t
ORIGIN      
        1 tttcctccca taaagcagat ggcaagacct ggctcgacgt tcatcggacc gcccatagcc
       61 taggattgaa aagcaatgct accatgttgt ttggacatgt ggaaacaatc taccatcgtg
      121 tggatcatct ccttgaactg cggacacttc aggacgagac cggggggttt caggcgttcg
      181 ttgccttgcc atttcatccg gtgaggacgg ccttttctca tttgccgaag ccgtccgcat
      241 tcgactgtct tcgaacaatc gcggtctccc ggctcttgct ggataatttc gaccatatca
      301 aagcgtactg ggtagccttg gggatcggtg tggcccaaac tgccttagaa tatggggcca
      361 acgatctgga cgggacagtg cgatatgaga atatttatca tgatgccggg gccgattctc
      421 ccgatgtcct atcggtcgag gagttgtgcg cgctcattcg ggagactgga cgcgagccgg
      481 tggagcgaga cagcctttat cgccgtgttc ggcgcttcgg acgggaatgg gaattggaag
      541 acaatcaccc cgatgttcgt aatcgagaat gaatcgttct gtcggtgaca cccctgctcg
      601 gaacattgct gggttgtgcg aagcctgtca gaatcatgat gaacgaggga acaaagccat
      661 ctacgatctc gccaagggat caggatcccc tccggcttcc catccaattt gtaaaaggcg
      721 taggaccacg gcgggctgag atcctggcca aactggggat tgaaaccgtc agcgatgccc
      781 tcttcttttt tccgcgatct tatgaggatt gcagcagggt acggccggtg aatcaattgg
      841 aagaaggaac cgtccaggca gttgtgggtg ttgtcgaaga cgtggagagc cgggtcctcc
      901 ggggtggcca gactgtcgtg ggtgtgctga tcgctggaga gggtggatac gtccgggctg
      961 tgtggtttca gcagccctac cgccgggatt attttcgcga aggtcaacgt gtcatggcgg
     1021 tgggaaaacc gcggcgcaat gggttgattt gggagatggt tcatccccgg gtcattgttt
     1081 taggagagga ggaacaacca aaactagccc ctcttcctgt ctatgccttg accgagggaa
     1141 tccaacaatg ggaaatgcgc aaaatgattg ggcgggtggt ggcccaatat agcggatatt
     1201 tggaagaggt gcttcccgag aaaatcctcg cccaacagcg actcttgcca attcaggagg
     1261 cagttcagca gattcacgcc cccagtgatc agcagcaaat ccaagccgca cggcgtcggt
     1321 tcatttacga agagctattc atcctccaag tggccttggc cttgaagcgg tatcagcaga
     1381 tcactagtaa aaaatcgccc atccttcgat tgactcccaa aattgacgag agaattcgtc
     1441 ggctctttcc ttttagattg actgccgccc aagaccaggc cgttgccgag attgttcgag
     1501 acatggagca gccgattccc atgaatcggt tgcttcaagg ggatgtggga agtggaaaaa
     1561 cagtcgttgc tgtttatgcc atgctcttgg ccgtggcaaa tcagcatcag gctgcgatga
     1621 tggcgcccac cgagattctt gcccggcagc attttcagac tattaaccaa ctgctcgaga
     1681 aaagccgtgt ccgacggaca ttgctcgtcg gtgggctacc gccccaagag cgatcccaga
     1741 ttcttcggca gatcgccgag ggtgagatcg atttggtggt gggcacacaa gcaatcatcc
     1801 aggaagaagt cgagtttcat cgacttggcc ttgtcgtgat tgacgaacaa cacaaatttg
     1861 gggttcgcca gcgggcggct ctgaaagggg c
//
LOCUS       DAOPTR010000841         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_47923_length_1888_cov_2.857065, whole genome shotgun sequence.
ACCESSION   DAOPTR010000841 DAOPTR010000000
VERSION     DAOPTR010000841.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47923_length_1888_cov_2.857065"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..>1888)
                     /locus_tag="PKI05_13740"
     CDS             complement(115..>1888)
                     /locus_tag="PKI05_13740"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOA53311.1"
                     /translation="GLVGTESLPCLLGGKDPHVVEEKSETSRGARTHFLEALQDSFGT
                     AFSVYDGVSGELQTANQELRIDWELFGILCRVVSQRRQPEIVWEEDPFIFLAVPYQQS
                     ADEERVAVGAFVSRFVGLDEDLSLQARSLSGDPQRIRDWAIRQTPWQPEALLRVATAS
                     LRQLRQRTELALLEKQMEEMSVHMAAVYEEISLLHRLTRDLRLSKSDEALAETAATWL
                     LEIVPAEGIGAVFLPVAEQDDNFLGNIRTKSVLLTAGDFPFSADTFLALADHFQVRSS
                     KDPVVKNFVEAPFHFAGVQLRNLIAVPILEGENLFGYLAAVNHVQGGQFGSMEANLLH
                     SVATIIGVHSGNIDLYRQQAELLAGVVKALTSAIDAKDPYTCGHSDRVARIAVRLAQE
                     LGYRPETLSRVYLAGLLHDIGKIGVNDAVLQKPGKLTDQEFELIKTHTKTGFRILSGL
                     KQLEDINKVVLHHHEAWNGSGYPEKLVGEAIPQIARIVAVADAYDAMASDRPYRTRMP
                     NDKIDEIIRAGAGRQWDPEVVEAFFRARDDLREIADEKDLKFLNHPLLGPMIRKSLGL
                     PENGNGNSTTAGSQPESEASKRAP"
BASE COUNT          411 a          542 c          544 g          391 t
ORIGIN      
        1 ggcttcgaaa ccgcggttac cggtagagga aaccgggggg atctacggaa aggacccgca
       61 attgagagaa acacaaggcg gctggtggaa ggccgagaat ccagccgccg ctgcttacgg
      121 tgcgcgcttg gaggcttccg actcgggttg actcccagcg gtcgtgctgt ttccatttcc
      181 gttttccggc aggccgaggg actttcggat catcggcccc aggagaggat gattgaggaa
      241 ctttaggtcc ttttcgtcgg cgatctcacg aagatcgtcg cgcgcccgga aaaaggcttc
      301 gacaacttca ggatcccact ggcgaccagc cccagcccgg atgatttcgt cgatcttgtc
      361 gttgggcatc cgggttcggt aaggacggtc actggccatg gcgtcgtagg cgtccgccac
      421 ggccacaatt cgggcaatct ggggaatggc ctcgccgacg agcttttccg gataaccact
      481 gccgttccag gcttcgtggt ggtggagaac aaccttgtta atgtcctcga gttgctttag
      541 ccctgagaga atgcggaagc cggtttttgt gtgggtcttg atcagctcaa attcctggtc
      601 ggtcaatttg ccgggctttt ggaggacggc atcgttaacg ccgatcttac caatgtcgtg
      661 cagaagacct gccagataga ctcgcgacag tgtctccgga cggtaaccaa gctcctgggc
      721 caaccggacg gcaatgcgag ccacacggtc actgtgaccg catgtgtagg gatccttggc
      781 gtcaatggcg gaggtcaacg ccttcaccac acctgcaaga agctcggcct gctgccggta
      841 taaatcgatg ttgccgctgt gaactccaat aatcgtggcc acggaatgca agaggttcgc
      901 ttccatgctt ccaaactgtc ccccttggac gtgattaacg gcggccagat agccaaaaag
      961 gttctcgccc tcaaggatcg gcacggcaat caagtttcgt agctggaccc cggcgaagtg
     1021 gaatggcgct tccacaaagt tcttaacaac agggtcctta gaggaacgga cctgaaaatg
     1081 atcggccaac gcgaggaagg tgtccgcact gaacgggaaa tccccggccg tgagtaacac
     1141 cgacttggtg cgaatatttc ccagaaagtt gtcgtcttgt tcggcgacag gcaaaaacac
     1201 tgcgccgatt ccctcggcgg gtacaatttc caacagccag gtcgcggcgg tctcagcaag
     1261 ggcttcgtcg ctttttgaca accgtaggtc ccgtgtcagc ctatggagga gacttatctc
     1321 ctcatacacc gcggccatgt ggaccgacat ctcctccatc tgtttttcca acagggccaa
     1381 ttccgtccgt tgacggagct gcctcaggct ggccgtggcg acccggagca aggcctccgg
     1441 ttgccaaggc gtctgtcgaa tggcccaatc gcgaattcgc tgcggatccc ccgacaaact
     1501 cctggcttgc agggagaggt cctcgtccaa gcccacaaat cgggaaacaa aggccccaac
     1561 agccacccgc tcctcatcgg ccgactgttg atagggaacg gccaggaaaa tgaaagggtc
     1621 ttcttcccag acgatttccg gctggcgtcg ctgcgacaca acgcggcaaa ggataccgaa
     1681 taactcccag tcgatccgta gctcttgatt ggcagtctgg agctcgcccg aaaccccgtc
     1741 gtaaacggaa aaagcggtac cgaaggagtc ttgaagggct tccaagaagt gcgttctggc
     1801 cccacgtgat gtctccgatt tctcctcgac aacatggggg tcttttccac ccaggagaca
     1861 gggtaatgat tctgtgccaa ccaggccg
//
LOCUS       DAOPTR010000842         1886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_47975_length_1886_cov_3.822501, whole genome shotgun sequence.
ACCESSION   DAOPTR010000842 DAOPTR010000000
VERSION     DAOPTR010000842.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47975_length_1886_cov_3.822501"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            287..865
                     /locus_tag="PKI05_13745"
     CDS             287..865
                     /locus_tag="PKI05_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53312.1"
                     /translation="MQSGITLATWFKRESRRVHGLFTETDEEADQRRLIEWLEARGGA
                     ATVRDLTRGPRRFRNKPNEAEAALTALVNAGLGHWESPPSNQKGGVQHVFFALSPSVT
                     VTKTPLKDLKTKVSVTVTVVTPKLHGPRRRKRLLLSPPYVAMMPARLVFPRMEVMMTA
                     IGWRSPYESDRATLSAWRGRGLGGWGTEITHP"
     gene            1549..1824
                     /locus_tag="PKI05_13750"
     CDS             1549..1824
                     /locus_tag="PKI05_13750"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOA53313.1"
                     /translation="MKRLRSIAAKISDAGNTSTQIPKLALRPDELATAFGVSRRTICE
                     WTSRGLLPCIRQGRVVLYPVSLLQEWLERQIQTAAPQGNTAESGHDR"
BASE COUNT          434 a          502 c          540 g          410 t
ORIGIN      
        1 tggcatggtt ctggcgagct gtgaccggtc aagggagagc aaaaccaaat ttggcatggt
       61 ttactggggg ggcgatggag gggccggtgc ttatcccctt tcttttgctt atgggtcatt
      121 cgtagcgaaa aattctccga ggggcccccg ggaccagtca cgaaactaag cacagtttgt
      181 ttgtacactg ccgtgggtcc ttccggatac gaaactccgt cgttttatta acgctgatac
      241 cgcgcgcaga atctaaaaaa ccacggatgc cgtggatgcc gcaagcatgc aatccggcat
      301 tacattggca acgtggttca aacgcgaatc ccggcgcgtc catggtttat tcacggagac
      361 ggacgaagag gccgaccagc ggcggttaat tgaatggctt gaggcccgag ggggagcggc
      421 aacggtccga gaccttaccc gtggaccacg tcggtttcga aacaaaccta atgaggcgga
      481 agcggccctt accgcccttg tgaacgccgg gctggggcat tgggaatcgc caccgtcaaa
      541 ccaaaagggg ggcgtccagc acgtgttttt cgccttgtct ccaagtgtca ccgtcaccaa
      601 aacccctcta aaagacctga aaacgaaggt ttcggtgacg gtgacagtgg tgacaccgaa
      661 acttcacggc ccacgacgac ggaaacgcct attgttatcc ccaccatacg tggcaatgat
      721 gccggcgcga ttggtgttcc cacggatgga agtgatgatg acggcgattg gttggagatc
      781 accttatgaa tccgatcgag ctactttatc ggcctggcgt ggccgtggat tgggtggatg
      841 gggaactgaa attacgcatc cgtaaggggg cgttgaccaa cgccacccgg cctgaccttt
      901 tggagaaggg ttgtccaacc tgagcaggac taccccgccc ggccctccca aagggaaatt
      961 catttgtggc gcttctgtag acgcgcatga agacgctgat gtagacgcgc gctgtaacat
     1021 cgcagcgtta tgggccgggc ctgtcacccc cacattgtcc ttggacaaag tttacccaga
     1081 ttaccacaac gcaacaaaac acagcgcgct ccggagaaaa taggtaaagt tttctcacgg
     1141 gggcatttgc ggaattaagg agcgcggccg ttgcttagac caagactttc cggagaaatg
     1201 acaaccagag ccactatcca cacttcacta atctcgatct tgtcttcgtt aatgcttgtg
     1261 aagtcttcaa tactcccagt cttggaagtt tctgtctagt ttccttgaac tcttgctagt
     1321 ctccaacagg tttttcgcaa aaaacgatcg tcttatctct cgaaataacc gtaatttacg
     1381 gttcgcgcat cgcggaggtg gccgaatttt ggcatacccc cctgttatac tcgatggcat
     1441 gaacacgcgc ccatccgttg gggcgcgaac ggggacgcat gacacggatg acataaggct
     1501 tggcaggagc gtggtttgtg tttggcgaat ggaggtgacg cgatgaacat gaagagactt
     1561 cgaagcattg cggccaagat cagtgatgcg ggtaacacga gcacgcaaat ccccaaatta
     1621 gccttgcggc ccgatgagct tgccacggcc tttggcgtga gccgtcgaac tatctgcgaa
     1681 tggacatcac gcggccttct cccgtgtatc cgtcaaggcc gagtggtttt gtatcccgtt
     1741 tctctgctgc aagagtggct tgaacggcag attcaaactg cggccccgca aggcaacacc
     1801 gcggagagtg gccatgaccg ttgacgaggt agttgcacta ctggagcggc acggatgcca
     1861 cccacgccgg gccggcgccg gctggg
//
LOCUS       DAOPTR010000843         1886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_47981_length_1886_cov_3.380120, whole genome shotgun sequence.
ACCESSION   DAOPTR010000843 DAOPTR010000000
VERSION     DAOPTR010000843.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47981_length_1886_cov_3.380120"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1857)
                     /locus_tag="PKI05_13755"
     CDS             complement(<1..1857)
                     /locus_tag="PKI05_13755"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOA53314.1"
                     /translation="MPAHFTLRTMTLAAAALGLVALLQGADIRVDITSERISSLSPHT
                     IELLRSLDPKHVVQVEAFISPEVPESYIQTRANLVNLLREFEARAKNVRVRINDTEPF
                     TAEAQRAADRFNITPQRVFALQQGRWTEHRIFMGVAFTCGLEKVVVPFIDRDIPVEYE
                     LIRSIATVTQQKRKRVGILTTDAQLFGQFSFQGGQERWPIVDELQKQYEVVRVEPSDL
                     VTNDEECEKAGMKVLGLTDASEDKIIEKGRELLGLDTPIDKLSDQQKQQCKDKVKEEA
                     MKLGRWDVLLAVQPSSLAPDDMDRFVAAISYGQPTVIFEDPMPIFAGGVPGTGQERRP
                     PTQMMMFGMRPLPKGDIGKLWRKLGIDFTTNEIVWQRYNPYPKFELFEQNPEFVFINR
                     SCGAEQPFNEQEPAVAQLQQVLFPFPGAINRLNSSTMKFTPLVKTGRITGLVNYDDIF
                     RMDFLTGSRELNTNRPLRPTRTEYVLAATIEGKLPEMKITKSEESNPEPGASSNKVEE
                     LADKQHDVRVILVADIDMLHETFFRLRQRQDLPGLDLKLSFDNVTMVLNLIDRVSGDD
                     RFIDIRNRRPKHRTLTTIERATEAARARTAEERQKLQEAYNQIEKEEREKLEQ"
BASE COUNT          402 a          540 c          486 g          458 t
ORIGIN      
        1 ctgttcgagc ttttcccgct cttctttttc gatttggttg taagcctctt gcagcttttg
       61 gcgttcttct gcggttcggg cccgcgccgc ttccgtcgcc cgctcgatcg tggtcaacgt
      121 gcggtgtttg gggcgtcggt tacggatatc aatgaagcga tcatctccac tcacccgatc
      181 aatcaggttg agcaccatcg tgacgttatc aaagctcagc ttgaggtcta agccgggcaa
      241 atcctgtcgt tgacgcagac ggaagaatgt ctcatgcaac atatcaatgt ccgccacaag
      301 gataacccgg acatcgtgtt gtttatctgc aagctcctcc actttgttgc tgctagcgcc
      361 gggttcggga ttactttcct ccgacttcgt gattttcatt tcggggagct ttccctcaat
      421 ggtggccgcc agcacgtatt cggtcctggt cggacgcaaa ggacgattcg tgttgagttc
      481 ccggctaccg gtcagaaagt ccatgcggaa aatgtcatca taattgacca gaccagtgat
      541 tcttcccgtc ttcaccagcg gcgtgaactt catggtggaa ctgttgagcc gattgatcgc
      601 cccggggaac gggaagagga cctgctgcaa ttgcgccacg gccggttcct gctcattaaa
      661 cggctgttct gccccacagc tccggttgat gaagacaaac tccgggttct gctcgaacag
      721 ctcaaacttg ggatacggat tataccgctg ccaaacgatc tcattcgttg taaagtcaat
      781 gccgagcttg cgccacagtt ttccgatgtc gcctttcggc aaggggcgca taccgaacat
      841 catcatttgc gtaggtggac ggcgctcttg acccgttccg ggcacccctc ctgcgaagat
      901 cggcatcggg tcctcgaaga tgacggtcgg ttgaccatag ctaatagccg cgacaaaacg
      961 atccatgtca tcgggcgcca acgaggaagg ctgcactgcg aggagcacat cccatcggcc
     1021 gagcttcatg gcctcctctt tgaccttgtc tttgcattgc tgcttttgtt gatcactgag
     1081 cttgtcgatt ggcgtatcaa ggcccaaaag ctcccgacct ttttcaatga tcttgtcctc
     1141 cgacgcatcc gttagtccga ggaccttcat tcccgccttt tcgcattcct cgtcattggt
     1201 caccaaatcg gacggctcca cacgaacgac ttcatactgt ttttggagct catccacaat
     1261 gggccagcgc tcctgacctc cctggaaact aaactgtcca aaaagttggg catcggtcgt
     1321 gaggatacca acccgtttgc gcttttgctg ggtcaccgta gcgatggatc gtatcaactc
     1381 atactccacg ggaatgtccc gatcgataaa gggcacgacg accttttcca aaccacaggt
     1441 gaaggccacc cccatgaaga tacgatgctc cgtccaccgg ccttgctgca gggcaaatac
     1501 ccgctgtgga gtgatgttga acctatccgc cgcccgctgg gcctctgccg tgaacggctc
     1561 ggtgtcattg atacgaacac gaacattttt agcccgggct tcaaactccc ggaggagatt
     1621 caccaggttg gcacgggttt gaatataaga ctcggggacc tctgggctga taaaggcctc
     1681 tacctgtacg acgtgcttgg gatcgagaga ccgcaacaat tcgatcgtgt gaggcgaaag
     1741 agaactgatt cgttcactcg tgatgtcaac acgaatgtct gcgccctgaa gcaaggccac
     1801 cagccccaat gctgccgcag ccaaggtcat cgttcgtaac gtaaaatggg caggcattaa
     1861 agagacagcc cggggaaatt gccacc
//
LOCUS       DAOPTR010000844         1884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_48067_length_1884_cov_3.204483, whole genome shotgun sequence.
ACCESSION   DAOPTR010000844 DAOPTR010000000
VERSION     DAOPTR010000844.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48067_length_1884_cov_3.204483"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..1031)
                     /locus_tag="PKI05_13760"
     CDS             complement(9..1031)
                     /locus_tag="PKI05_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOA53315.1"
                     /translation="MKIGFTYDLRSEYLAEGFSEEETAEFDRDDTIQAIEDALRAEGH
                     QVDRIGRIHNLVRRLAAGDRWDLVFNIAEGLYGLGREAQVPALLDAYQIPYTFADPAV
                     LMLSLDKALCKLVVHEAGLPTPAHAVVRCEADVEQVRLPFPLFVKPLAEGTGKGISPQ
                     SKVDTPQALEAACLSLLARFRQPVLVERYLSGREFTVGILGTGKKAKVLGTMEIILRP
                     QAEAHAYSYVNKEFCEELVEYRYPSAESDPEVAEAERVALAAWQAIGGRDAGRVDLRS
                     DETGQPHFLEVNPLAGLHPEHSDLPMIAKAKGLSFRELIRRIIASALERVEASCQTPN
                     CCPAGP"
     gene            complement(1031..1738)
                     /locus_tag="PKI05_13765"
     CDS             complement(1031..1738)
                     /locus_tag="PKI05_13765"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOA53316.1"
                     /translation="MGDEASLCSRCAQRGKTCCQICEIYVTPGDVQRIGTFVGHFDFF
                     EFRKPVDPQYLDQDDDPEWARCVFRPDGSRRVLRRSTSGDCVFLGPVGCRLPWEVRPL
                     VCRLYPVEYTASGLKPDLADGCPVNLLPPGQDLLQALGMTWEKAEVWWRQLYAEIRLE
                     PHLFDTGERDKIAPVEANIFPVRVDGPEADPPAPGGPGGSVLAQTPVGPTASQTGHKS
                     NDLGKPSSYPPAVAKAP"
     gene            complement(1753..>1884)
                     /locus_tag="PKI05_13770"
     CDS             complement(1753..>1884)
                     /locus_tag="PKI05_13770"
                     /inference="COORDINATES: protein motif:HMM:NF023958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53317.1"
                     /translation="GGGGKIPLQPNYVVGREGDELILRNYEGAIFRYPDPLEQSLDK"
BASE COUNT          403 a          613 c          528 g          340 t
ORIGIN      
        1 cccgcatgtc acggccccgc aggacaacag ttcggcgtct ggcaagatgc ctccacccgt
       61 tccaaggccg aggcgataat cctccgaatc aactcccgaa aagataaccc cttagcctta
      121 gcaatcatag ggaggtccga gtgctcggga tggagaccgg caagggggtt gacctccaaa
      181 aagtggggct ggcccgtctc gtccgagcgg aggtccacgc gaccggcgtc gcgcccgcca
      241 atggcctgcc atgccgccaa ggccacacgt tccgcctcgg ccacctcggg atcactctcc
      301 gccgagggat accggtattc cacaagctct tcacaaaact ccttgttaac gtaggaatag
      361 gcatgggcct ctgcctgcgg gcggagaatg atctccattg tccccaagac cttggccttt
      421 ttgcccgttc cgaggattcc cacggtgaac tctcgccccg agaggtaccg ctcaaccagc
      481 accggttgcc gaaaacgcgc tagcagcgag agacacgcgg cctcgagggc ctgcggcgta
      541 tcaaccttgg actgcggcga aatccctttc cctgtcccct ccgccagcgg cttgacgaaa
      601 aggggaaagg gcaaacggac ctgctcgaca tcggcctcgc agcgaaccac cgcgtgggca
      661 ggggtgggca gccccgcctc atgaacgacc aatttgcaaa gggccttatc caggctgagc
      721 ataagcacag ccggatcggc gaacgtgtag ggaatctggt aggcatcgag gagggcaggc
      781 acctgggcct cccgtcccag gccgtataac ccctccgcaa tgttgaacac caaatcccat
      841 cgatctccgg cggccagtct ccgcacgagg ttatgaattc gcccgatccg atcgacctga
      901 tggccctcgg cgcgcagggc gtcttcgatc gcttgaatcg tgtcatcgcg atcgaactcg
      961 gccgtctcct cctcggagaa accttccgcc aggtattcac tccgtaagtc gtaggtaaag
     1021 ccgatcttca tcagggtgcc tttgccaccg cagggggata ggaagatggt tttccaagat
     1081 cgttcgattt gtggcccgtc tgggatgcgg tcgggcctac cggcgtctgg gcgagcaccg
     1141 atccaccggg gccaccggga gctggcgggt ccgcttccgg cccatctacc cgcactggga
     1201 agatgttggc ctcaaccggg gcgattttgt cccgctcacc cgtatcaaag agatgcggct
     1261 ccaggcggat ttcggcgtaa agctgccgcc accagacctc cgccttttcc caggtcatac
     1321 ccagcgcctg aagtaaatcc tgcccgggag gcagcaagtt cacggggcag ccgtccgcca
     1381 gatcgggctt caagccactg gctgtgtatt ccaccggata aaggcgacac acaaggggcc
     1441 gaacttccca gggaagccgg caacccacgg gtcctaggaa gacacagtcg cccgaggtgg
     1501 aacgccgcaa cacacgacga gaaccatcag gccggaacac gcaccttgcc cattccgggt
     1561 cgtcatcctg gtccagatat tgtggatcca cgggcttgcg aaactcgaag aagtcaaagt
     1621 gaccaacgaa cgtcccgatc cgttgaacat cgcccggagt gacgtaaatc tcacaaattt
     1681 ggcagcatgt tttgccccgc tgcgcgcacc gcgaacacaa agatgcttca tctcccatga
     1741 tccacccctt tcttacttgt ctaaggactg ttccaaggga tcgggatacc ggaaaatggc
     1801 accttcgtag ttgcggagaa tgagctcatc accctcgcga ccaacaacat aattcggctg
     1861 gagcggaatc ttgccgcccc cacc
//
LOCUS       DAOPTR010000845         1884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_48076_length_1884_cov_2.790596, whole genome shotgun sequence.
ACCESSION   DAOPTR010000845 DAOPTR010000000
VERSION     DAOPTR010000845.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48076_length_1884_cov_2.790596"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..>1884
                     /locus_tag="PKI05_13775"
     CDS             17..>1884
                     /locus_tag="PKI05_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53318.1"
                     /translation="MTDHETGWRDGGESFTFRTRAAPGKGGDEGQRWYAEKMHELGFR
                     YGIYNNYTDYAPVNEYWDEDCVTRLSDGNWRRAWPRCYNPKPTRAVEFEAKLAPIIQE
                     KFHLSTAYCDVHTAVRPWDYCDFDARVPGAGTFAATFYAYGEIMLHQKATWNGPVYSE
                     GNNHWYYCGLTDGNYAQDQVARLDINPWLVDFDLRKLHPLCCNFGMGNMGMFYPRGPE
                     GLDPETALDRFLAATLAFGHTGFLVMEGGMANAVRSYYSVQQIHARYAQATVEDIRYR
                     DAEGNMLPTAQALATGAFRRSQVYVKYANGLQLWVNGNMSETWDVPDVNMTLPPNGWY
                     ARGSFPDGVLVAYSALVDGHRADYVDSPGYLYADGRGKVTRFPKAVCDGQIVLLPQAD
                     GSFELIPLGCTVMGVFLDGRSAQAIALDEENRKELGPAEVRYSRAFVHVVPQEGAFSY
                     KLRPEPAPQVSLWCDRESVVPGETLVIFGPDAVKWTVPPDAAPRAHIWYQQDGRWLDF
                     RVVPLADLELTVKPQQWLLRARSNLPQEQEFSITLAEHTQKVLFPPGVWQDVLFPFVP
                     VEQEQVIDIPVEVSGGGCRLLKHWQLRCEKKRVEVVGFPSIQRTVQAFRQQEEQPV"
BASE COUNT          403 a          523 c          548 g          410 t
ORIGIN      
        1 catgacgaag atcgttgtga ccgatcacga gacgggctgg cgggacggcg gggaaagttt
       61 caccttccgc actcgggcgg caccggggaa gggaggcgac gagggccaac gctggtacgc
      121 cgaaaagatg cacgaactcg gattccgtta cggcatctac aataactaca ccgattatgc
      181 ccccgtcaat gaatactggg atgaggactg tgtaacaagg ctttccgacg ggaattggcg
      241 ccgggcgtgg ccgcggtgtt acaatccaaa acccacccgc gcggtcgaat ttgaggccaa
      301 actggctccc atcattcaag aaaaattcca cctcagcacc gcgtactgcg atgtccacac
      361 ggccgtgcgg ccatgggatt actgcgattt tgatgcccgt gtgcccggtg ccggtacgtt
      421 cgccgcgaca ttttacgcct acggtgaaat catgcttcac cagaaggcca cttggaacgg
      481 tccggtctat agtgagggca ataaccactg gtattattgc ggtctgactg atggaaacta
      541 cgcccaggat caggtagccc gactggacat caacccctgg ctggtcgatt ttgatttgcg
      601 aaaactgcac cccctgtgtt gtaactttgg catgggcaac atggggatgt tttatccccg
      661 cggaccggaa ggacttgacc ccgagacggc actcgatcgc ttcctggcag ccaccttggc
      721 cttcggccat acaggctttc tcgtcatgga aggcgggatg gccaacgccg tgcgcagcta
      781 ttattccgtt cagcaaatcc atgcccggta tgcccaagcg acggtggagg acatccgtta
      841 tcgagacgca gagggaaata tgctccccac cgctcaggcc ctagctacag gcgcattccg
      901 tagatcgcaa gtgtacgtca aatacgcgaa tggccttcag ctttgggtca atggaaacat
      961 gtccgagact tgggatgtcc ccgatgtcaa catgacgtta ccgcccaatg gctggtacgc
     1021 tcgcggcagc tttcctgatg gtgtcttagt ggcgtactca gccctggtcg atggtcaccg
     1081 tgcggactac gtggattcgc cagggtatct ttacgccgac gggcgtggaa aagttacccg
     1141 atttcctaag gccgtttgcg atgggcaaat tgtcttattg ccccaggcag atggttcctt
     1201 tgaactgatc cctttggggt gtaccgtcat gggcgttttt ctagacggac gctcggccca
     1261 agccatcgcc ctcgatgaag aaaatcgcaa agaattggga ccggccgagg tccggtatag
     1321 tcgggcattt gttcatgtgg taccgcagga aggggccttc agttacaaac tccgaccgga
     1381 accggcaccc caagtatcgc tttggtgcga ccgagaatct gtagtgcctg gtgagacttt
     1441 ggtcatcttc gggccggatg ccgtgaaatg gactgtgcca ccagatgctg cgccccgtgc
     1501 ccacatttgg taccaacaag acggccgatg gctggatttc cgcgtggtcc ccttggctga
     1561 tctggagctg acggtgaagc cccagcagtg gctattgcgg gcacgatcaa acctgcctca
     1621 agagcaagag ttctctatca ccttggccga gcatacccaa aaggtattat ttccccctgg
     1681 cgtgtggcag gacgttcttt ttccctttgt cccggtggaa caggagcagg tgatcgacat
     1741 ccccgtggag gtgagcgggg gcggatgtcg cctcctcaag cattggcaac ttcgctgcga
     1801 aaagaaacgc gtggaggtcg ttggcttccc ctccatccaa cgaacggtgc aggcttttcg
     1861 tcagcaggaa gaacaaccgg tgag
//
LOCUS       DAOPTR010000846         1883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_48098_length_1883_cov_3.410832, whole genome shotgun sequence.
ACCESSION   DAOPTR010000846 DAOPTR010000000
VERSION     DAOPTR010000846.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48098_length_1883_cov_3.410832"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PKI05_13780"
     CDS             complement(<1..181)
                     /locus_tag="PKI05_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53319.1"
                     /translation="MDKVKIAAEFDPTADFVLYEGNCLDLLPRIPDGFVKLVVTSPPY
                     NLGKPYETRLDMDAYL"
     gene            complement(748..>1883)
                     /gene="hypD"
                     /locus_tag="PKI05_13785"
     CDS             complement(748..>1883)
                     /gene="hypD"
                     /locus_tag="PKI05_13785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOA53320.1"
                     /translation="RGRRGTSGYGEKGVRFISEYRDSTAVRHWLRELQNLVTRPWTIM
                     EICGGQTHSFLRFGLDELLPKEITLVHGPGCPVCVTPVELIDKAVAIASRPEVIFCSF
                     GDMLRVPGSKDDLLRVKSRGGDVRIVYSPLDAIELAKQHPDRQVVFFAVGFETTAPAN
                     AMAVLQAAEAGLKNFSVLVAHVRVPPAIEAILSSPRNHVKGFLAAGHVCTVMGTEEYL
                     PLAKRYHTPIVVTGFEPADLLQGLAMCVAQLEAGKAEVEIQYSRSVRPEGNIVARRNI
                     DQVFRIVDRKWRGIGMIPQSGLALRDEFLDFDAEQRFATADVTAEESPECIAGEVLQG
                     HKKPMACPAFGTRCTPAHPLGAPMVSSEGACAAYYRYRRHEAG"
BASE COUNT          431 a          550 c          491 g          411 t
ORIGIN      
        1 tcagatacgc atccatgtcc aggcgagttt cgtaaggctt gcccagattg tatggtggtg
       61 aagtgactac gagcttgaca aatccatcag ggattcgagg aagcaaatcc aggcaattac
      121 cctcgtatag aacaaaatcc gccgtcggat cgaattcagc agcgatcttt accttgtcca
      181 tagccccccc tttttagttt ggtctttaag cataccaccg ctttggataa cttatgtgct
      241 cgactcctgc accgtacaaa gcgaggattt tcagcgaatc aaaacgccag aaaagtagag
      301 tgatggagtt gcttttgaga tgcatttttg gcaccagcaa tacacaacgt accctggcta
      361 acagatcctt gctagccaaa agcgactccc tccatcgggc aaatcgcccc cgaattccat
      421 cttgaattgg cgttgaaact tgtcaaagag gacctggctg tactggacgg ttctcagtaa
      481 gccggacaga acggcggcca aaacgaaagc cccactatat tgctcgagaa tccaaacctt
      541 atcctgaacc agccaatcta ttccgcctct gcgcccagga ctccagcacg tcagtgctgc
      601 ccgcgggggc cggtgccgcc tgctgtaaac aacggtatgg taaccgatgg tactgtgaat
      661 gtcaattcac cgtcttgcga tcgttcgaac actcaaccaa tgaatttcct ttttcttgtc
      721 cacagccggt tatggcagcg gcctcattca gccggcttcg tggcggcggt agcggtaata
      781 tgccgcacac gcgccttcgg acgaaaccat cggggctccc aggggatgtg ctggagtaca
      841 gcgtgtacca aatgccgggc aggccatcgg tttcttgtgc ccttgaagga cctcgcccgc
      901 tatgcactcc ggcgactctt cggccgtcac atctgccgtg gcaaaacgtt gctccgcgtc
      961 gaagtcgagg aattcatccc tcagtgcgag gccactttgt gggatcatac cgatgccgcg
     1021 ccatttgcga tcgacgatcc taaagacttg gtcgatgttt cgacgggcca caatattgcc
     1081 ttccggtcgc acggaacgtg agtactggat ttccacctcg gccttgccgg cctccagttg
     1141 cgccacgcac atggctaagc cctgaaggag gtcggctggt tcaaagcccg tgacgacgat
     1201 gggcgtgtgg tagcgctttg ccaggggcag atattcctcg gttcccatca ccgtgcaaac
     1261 gtgtcccgcc gccaggaaac ccttcacgtg attccgcggc gacgagagga ttgcctcaat
     1321 tgccggggga accctgacgt gcgccaccaa cacggaaaag tttttcagac cggcctcggc
     1381 agcctggagg acggccatcg cattggccgg cgcggtcgtc tcaaacccca cggcgaaaaa
     1441 gacgacttgg cggtcggggt gttgtttggc caattcaatc gcatcaagag gagagtacac
     1501 aatacgaaca tcgccaccgc gcgatttgac tcgcaataga tcgtcttttg agccgggaac
     1561 gcgtagcatg tcgccaaagg agcaaaaaat gacctctggt cgggatgcaa tggccacggc
     1621 cttgtcgatc aattccactg gcgtcacgca cacaggacac cctggcccat gaacaagtgt
     1681 gatttctttc ggcaaaagtt cgtccaggcc gaagcgcaag aaggaatgcg tctgcccgcc
     1741 gcaaatctcc ataatcgtcc agggccgcgt caccagattt tgaagctctc gcaaccaatg
     1801 acggacggct gtgctgtcac gatactcgga aatgaacctc acgccttttt ctccatatcc
     1861 tgaggttcct cggcgacctc gcc
//
LOCUS       DAOPTR010000847         1881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_48193_length_1881_cov_2.145674, whole genome shotgun sequence.
ACCESSION   DAOPTR010000847 DAOPTR010000000
VERSION     DAOPTR010000847.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48193_length_1881_cov_2.145674"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="PKI05_13790"
     CDS             complement(<1..1163)
                     /locus_tag="PKI05_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53321.1"
                     /translation="MRPSRGRMSHIAGFFGLWIALVGSLLSVAALYNRQTCNKPLICF
                     IRNTPPQTAETKLTSGKGPTYENPTRELQTEAPVGDPQNAHDDSAAVKASTQSPILDN
                     RLLAASDGIRMMTPAQMRRIEAVAAAWCHVTLSEVEARLLKDAQDGELKEFDLLTGAM
                     IAGGCPPQKLEFLLRKFADQMKRIQARVDPRMPPLVRLQQAFRALHSEILYGGYFIGA
                     SNPGEAIQSGRFNCVSAAILFKLVADSLGFEVRVLQSPTHAYCLVRCEDNWVPVECTC
                     PTWFDNLSNRDQLAQDVDRQNPGKTPSIGNRLEHPMVSRAQQSEGTPDPKSGRGISET
                     QLLGTIYYNRGVASLLDRQYEIAVVSNLRAIQLDPANHSAQDNFLASLNNWAIA"
     gene            1529..>1881
                     /locus_tag="PKI05_13795"
     CDS             1529..>1881
                     /locus_tag="PKI05_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL family lipase"
                     /protein_id="HOA53322.1"
                     /translation="MGKRRIGFLGRLLVSTLLITAGHAVLANDAINPVPRSDQWWMQR
                     HQSMNDRVKQGNVDLIFIGDSITHGWEGAGKEIWEAYYGHRNAVNLGIGGDRTQHVLW
                     RLDNGNIDGIQPKLAV"
BASE COUNT          452 a          488 c          512 g          429 t
ORIGIN      
        1 gcaattgccc aattgttaag gctggccaaa aaattgtcct gcgccgagtg attcgctgga
       61 tctaactgaa tagctctcag gttgctaaca accgcgattt catattgccg atccaagagg
      121 gacgcaactc cccggttgta gtagattgtc cccaaaagtt gcgtctcaga aataccccgc
      181 cccgatttcg gatcgggggt cccttccgat tgttgcgctc gggaaaccat gggatgctcc
      241 agccgattgc caatgcttgg cgtcttgccg ggattttggc gatcgacatc ttgggctaat
      301 tggtcccggt tggagaggtt gtcaaaccag gttgggcaag tacattcgac agggacccaa
      361 ttgtcttcac accttaccaa gcaataagca tgcgtcggcg attggagcac tcgcacttcg
      421 aaacccaacg agtcggcaac caatttaaat aggattgcgg cactgacgca gttgaaccgg
      481 cctgattgga tcgcctcgcc cggattactg gcgccgataa aatacccgcc gtacagtatt
      541 tccgaatgca gagcccgaaa ggcctgttga aggcgtacaa gcggcggcat gcggggatcc
      601 acccgtgcct ggattcgttt catctgatcc gcaaactttc gaaggagaaa ttcgagcttc
      661 tgcgggggac acccaccagc aatcatggct cccgtcaaga gatcgaattc tttcaactct
      721 ccatcctggg catccttcaa aagacgagct tccacctccg acagagtcac gtgacaccaa
      781 gcagcggcca cggcttcgat gcgacgcatt tgcgcgggcg tcatcatgcg aataccgtcc
      841 gaagcagcca gaaggcgatt gtctaatatc ggactctgtg tgctagcttt caccgcagca
      901 gaatcgtcat gtgcgttttg cggatcgccg accggtgcct ctgtctgaag ctcccgcgtg
      961 gggttttcgt atgtagggcc cttccccgac gtcagtttgg tctcggcggt ttggggcgga
     1021 gtgttccgga tgaagcaaat gagcggtttg ttacatgttt gccggttata caaagctgca
     1081 acagaaagca agctcccaac caaggctatc catagcccaa agaatccggc gatatgactc
     1141 atccgacccc tggatgggcg catggatgct ttccaatggc tgagaaaccg cggaatgcaa
     1201 actaattggc ggggaaagtc aaaactcatg gcaggtcaca cacggcaagc aattcgcacg
     1261 gaaatgaccg tgaacaagca cgagcaggac acttcatcaa accataggtt gcgatttgcc
     1321 ccgcgccaaa tgcgtcagag aacaggatag gccaactgtg gtgaacgagt ggttttacgt
     1381 tggaattgtt ttcttcagac cagcgcaact tctccactga cccaaaattc cgttgtgcct
     1441 aagtggacaa tacctcggcc gctcattaaa ctgagcggat tgattgcgag tcgtttttgt
     1501 aaaccttttg ctttggggag ttgcagctat gggaaaaagg cggattgggt ttctggggag
     1561 gcttctggtt agcacacttt tgataacggc cgggcatgcc gtcttggcca atgatgccat
     1621 caatcctgtg ccgcgatcag atcagtggtg gatgcaaaga caccagtcga tgaacgatcg
     1681 ggtaaaacag ggaaatgtcg atttgatctt cattggtgac tcgatcacac atggctggga
     1741 aggagcaggc aaagaaatct gggaggccta ttacggccat cgcaatgcgg tgaaccttgg
     1801 cattggcggc gatcggaccc agcacgtttt atggcgcctc gacaatggca acattgacgg
     1861 catccagccg aaacttgctg t
//
LOCUS       DAOPTR010000848         1874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_48420_length_1874_cov_2.953271, whole genome shotgun sequence.
ACCESSION   DAOPTR010000848 DAOPTR010000000
VERSION     DAOPTR010000848.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48420_length_1874_cov_2.953271"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..817)
                     /gene="bshB1"
                     /locus_tag="PKI05_13800"
     CDS             complement(86..817)
                     /gene="bshB1"
                     /locus_tag="PKI05_13800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HOA53323.1"
                     /translation="MLLKDQDGISHVDSVVVAPHPDDAELGMGGTILKMVASGLTVGI
                     VDVTNGEPTPYGTPEIRAAETEQASRVLGIRWRINLGMPNRSLQPTLEGRAALAAVFR
                     LSRPKAVFCPYWEDAHPDHLATTEMTEAARFWAKLTKTDLPGDPYYPPRMYYYFCTHL
                     RKVFVPSFAVDITPFIDKKLEAVRCYESQVIMGRPTDPYSFVDSLRHQAAALGTAAGV
                     GFAEGFACRETLLVDDLRALFPVPR"
     gene            1048..1740
                     /locus_tag="PKI05_13805"
     CDS             1048..1740
                     /locus_tag="PKI05_13805"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOA53324.1"
                     /translation="MRLTFKVDRSLLIGLALMALSPAAFAVDLSLAVWAKEDAYPKLL
                     VKASNLSEFFAHGLGVLCIVFLVGLLDTKGWRRGMTVLIGAFSAGLTADLMKPLIFRV
                     RPRDFDFAGGILTTFQEWLPVATRQFTHLSSSFPSSHAATAAGLAFVLILLYPKAKPI
                     FTVLLVLSCAQRIVSSAHFLSDVLFGAGLGWCVSCLVVWYLVPSSWWSDQAPASRLGR
                     PAQRDESKGEAT"
BASE COUNT          395 a          496 c          533 g          450 t
ORIGIN      
        1 tcagcacggt agcccgcggt gtagttacca agggggagtc cacgcgtgag aacagctccg
       61 agctggagcg caacttcaac cgccctcatc ttggaacagg aaacaaagct cgcaggtcat
      121 cgaccaagag ggtttcccga caagcgaagc cttcggcaaa ccccacgcct gccgctgtcc
      181 cgagtgcggc agcttggtgc ctaagcgagt ccacaaacga gtacgggtcc gtaggacggc
      241 ccataatcac ttggctctca taacaacgca cggcttctag ctttttatca atgaacggcg
      301 tgatatcgac ggcgaagctc gggacaaaca ctttgcggag atgcgtgcaa aagtagtagt
      361 acattcgggg aggatagtag gggtctccgg gaagatcggt cttggttagt tttgcccaaa
      421 atcgggcggc ctcggtcatc tcggtagttg ccaggtgatc cggatgtgca tcctcccaat
      481 aaggacagaa cacggccttg gggcggctta atcgaaagac cgctgccaac gcggcccgcc
      541 cttccagggt tggctgcagg ctgcggttcg gcattcccaa attgatgcgc catcgaatcc
      601 ccagaacgcg actcgcttgc tctgtttcgg ctgccctgat ttcgggggtt ccataggggg
      661 tgggctcacc gttcgttaca tcgacaatgc caacggtcag gccggacgcc accattttga
      721 gaatggttcc tcccattccc agttcggcat catcagggtg gggagcgacg acaacgctat
      781 cgacatgaga gataccgtct tgatctttaa gtaacatagc ggtctggacc cgtcttacct
      841 aaaaaactgt tactcgccat tctacggtgt acgccaacct agacaatcca cctgcgaggg
      901 cacgtcactc gcaaggtaac gataggattg atgaaacacg aatggccgtt ccaaagtgat
      961 gtctctttgt catccgcggt ttggttagaa taggccaaag gtgagcgagt tctcgtaaca
     1021 gaagcaatca aacacgcgga gggccggttg agattgactt ttaaagtgga tcgcagtctt
     1081 ctaataggct tggcactgat ggccctaagc cccgcggcct tcgctgttga tctttcgttg
     1141 gccgtttggg caaaggagga tgcctacccc aagctattgg tgaaagcgag caatctcagc
     1201 gagttttttg cccatggact tggcgtcctt tgtatcgttt ttctcgtggg tttgcttgac
     1261 accaaaggat ggcgccgggg gatgacggtc ctgatcgggg ccttttcagc aggcttgacc
     1321 gcggacttaa tgaagccgct cattttccgc gtgcgtcccc gggattttga tttcgccgga
     1381 gggattctca ccacctttca agagtggcta cccgtggcta cccgtcagtt cacgcactta
     1441 agtagctcat ttccctcatc tcatgctgct actgcggcag gcttggcatt tgtgctcatc
     1501 cttctttatc cgaaagcgaa accaatcttc acggtattgc tggtcttgtc gtgcgcccaa
     1561 cggatcgtga gttcggccca ttttttaagc gatgtgctat tcggggccgg attaggttgg
     1621 tgtgtgagct gccttgtcgt gtggtactta gtgccctcgt cctggtggtc cgaccaagcg
     1681 cctgcttctc ggctgggaag acctgctcag agggacgaat ccaaagggga agcgacctga
     1741 atcggaagat tcgtttccga tcccgatttc acggggtttt agcgcggcaa agctcgactt
     1801 ggatttgatt ttccgagtcg cgggaaggga tcacaatttt ggcgtaataa ccggaggcgg
     1861 catggccgca gttg
//
LOCUS       DAOPTR010000849         1869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_48587_length_1869_cov_4.147740, whole genome shotgun sequence.
ACCESSION   DAOPTR010000849 DAOPTR010000000
VERSION     DAOPTR010000849.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48587_length_1869_cov_4.147740"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PKI05_13810"
     CDS             complement(<1..429)
                     /locus_tag="PKI05_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008694832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOA53325.1"
                     /translation="MASTERPSQNDLKHSGGAAHSPCRILGIDPGLKITGYGVVEVDE
                     TNHPHLCEAGVIRAPVRGDLAKRVKTIFDGVTEIIEAFQPSVVALEELYSHYERPRTA
                     ILMGHARGVICLAAAQRGVEVISYAATQVKRLLTGSGRAPK"
     gene            complement(500..>1869)
                     /gene="cysS"
                     /locus_tag="PKI05_13815"
     CDS             complement(500..>1869)
                     /gene="cysS"
                     /locus_tag="PKI05_13815"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOA53326.1"
                     /translation="VVNITDVDDKLIAESERRGIPMSELAEEMIADYMANLQALGIDG
                     IDHFPRATENIDAIITFTEDLIRKGYAYPAGGDVYFDVSKVPDYGKLSRRKLESMLPE
                     GGEGVERKRSPYDFALWKGAKPNEPFWNSPWGPGRPGWHIECSVMSSRLLGETFDIHG
                     GGLDLIFPHHENEIAQSEARHGKPMVRYWMHNGLMQAADEIGKVGGRNTRVAEDDLDA
                     QQAGKISKSKGASPFRDLLKEYRPETIRLFILSTHYRRPIEFSERRLQDTETAIETFY
                     RFFERFERITGQSFYSLPVPKSRKEGDLLDGNDAFMQDVAGHRARFLEFMDDDFNTGG
                     AIGELFELVRRLNRFVDEHRLEDPQHRTPEKTAALVRGATVLRELGHVLGLFRQPPPK
                     RQLPEEGGMVAKLLDLLVSIRSEARKAKNFQMADRIRSGLAELGVVLEDRPDGTQWRI
                     TDQTG"
BASE COUNT          382 a          560 c          508 g          419 t
ORIGIN      
        1 cttgggagca cgtcctgaac ctgtgagaag acgcttcacc tgtgtggccg cataggagat
       61 gacttcaaca cccctctggg ctgccgcaag acagatgacg ccccgggcgt gccccatgag
      121 gatagccgtg cgcggtcgct catagtggga ataaagttct tctagggcca ccactgacgg
      181 ctggaatgcc tcgatgatct cggttacgcc gtcaaaaatc gttttcacac gtttcgcaag
      241 gtccccgcgc actggtgccc gaatcactcc ggcctcgcac aagtgaggat ggttagtctc
      301 atccacttcg accacaccat agcccgtgat cttaagacca ggatcgatcc ccaagattcg
      361 gcaaggagaa tgggcagcac ctcccgagtg cttgaggtca ttttgcgaag ggcgttccgt
      421 cgaggccatc acgcttctcc gaggaaatag caagccgcgc tggggcacgg ccagcaaatc
      481 tcgctgaatc ttccatgtct cagccggttt ggtctgtgat ccgccattgc gttccgtcgg
      541 gacgatcctc taaaacaacc cccagttcgg cgagtccact gcgaatccgg tccgccatct
      601 ggaagttctt ggccttgcgt gcctcgctgc gaatggatac caagagatcg agcaacttag
      661 cgaccatgcc accctcttcg ggcaactgtc gtttgggcgg tggttgacgg aaaagcccta
      721 ggacatgacc cagttcccgt agtacggtag cgccgcgaac cagagccgcc gtcttttctg
      781 gggttcggtg ttgagggtct tccagccggt gttcatccac gaatcggttg agccgccgaa
      841 cgagctcgaa caattcgcca atggcccctc ctgtgttgaa atcgtcgtcc ataaactcca
      901 ggaagcgggc tctgtggccg gcgacgtcct gcatgaacgc gtcgttcccg tccaaaagat
      961 cgccctcctt tcggcttttg gggacgggta aggaatagaa cgactgacct gtgatccgct
     1021 cgaatcgctc aaagaagcga tagaaggtct cgatcgcggt ttccgtgtcc tggagccgcc
     1081 gctcgctgaa ttcgatcgga cggcgataat gcgtggacaa aatgaaaagc cgaatggttt
     1141 ctgggcgata ctccttcaaa aggtcgcgga atggactggc acccttggat ttgctaatct
     1201 ttccggcctg ctgggcatcc aagtcgtcct ctgcgacccg cgtattgcgg ccacccacct
     1261 ttccaatctc gtccgccgcc tgcatcaagc cattgtgcat ccagtaacgc accatgggct
     1321 tgccatggcg tgcttcgctt tgagcgatct cgttctcgtg gtgaggaaag atgaggtcca
     1381 atccgccccc gtgaatatcg aacgtctcgc ccaaaaggcg actgctcatg acagagcact
     1441 cgatatgcca gccaggacgt cccggtcccc agggactgtt ccaaaaaggc tcgttgggct
     1501 ttgccccctt ccaaagggca aaatcgtagg gggatcgctt tcgctccacc ccttcaccgc
     1561 cctcaggaag catggactcc aattttcgcc ggctcagttt tccgtaatcc gggaccttag
     1621 agacgtcgaa atagacatct cccccagccg gataggcgta tccctttcga atgagatctt
     1681 ccgtgaacgt tatgattgcg tcaatgtttt cggtggcccg cggaaagtgg tcgatgccgt
     1741 caatccccag ggcctggagg ttggccatat agtcggcgat catctcctcg gccaattctg
     1801 acatgggaat cccacgccgt tcgctttctg cgatgagttt gtcatcgaca tcggtgatgt
     1861 tgaccaccc
//
LOCUS       DAOPTR010000850         1867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_48664_length_1867_cov_3.602097, whole genome shotgun sequence.
ACCESSION   DAOPTR010000850 DAOPTR010000000
VERSION     DAOPTR010000850.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48664_length_1867_cov_3.602097"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="PKI05_13820"
     CDS             <1..1333
                     /locus_tag="PKI05_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53327.1"
                     /translation="VPFEGESVGEVLMKHLTKLPDLTPLPPEVRRVVGRALEKDPARR
                     FQDVREMVALLPGMEQSPYVEMGTAKAAGGPTEKEQFQSPGSAKMGHGEEPVMAELAE
                     ESNRPEPIWHAVSQAFRDLRTAWNNSRLATPLRVAILIGVAFLVISHVGILGPVIFGL
                     LMAYAVYWTVRAILIATMGQEHVVRGTAIPAELVAAPAQPTWPQRVAAGAPPPVPEPS
                     RAEAFRQYMSQMSFRAHLAETLGSFLAAFLIAGVLAVVGYLVQSFYGKTMTLTQASWV
                     FLITILGSWTILLVSKVWQVWESDASVRRFVMMVLGLALGIVGFGLTQYIQVDLTFNA
                     RWPNFHDWQPPASFYRDGQPMLPALMAAFGTHFLLVRWWRLADPLRRSRFCLWGVLGP
                     AIAAILVSAIWVFPGQWLLWPAVAMGITVQLAAPWFSIRRKRRSLPQRAHP"
     gene            1345..>1867
                     /locus_tag="PKI05_13825"
     CDS             1345..>1867
                     /locus_tag="PKI05_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53328.1"
                     /translation="MMLRVNLWVLLIMCALASRVLVAEPPSWVDARPQRVNGEYQIPI
                     HVGPYATVIECESNLHPIVQATIDDYVEQLIGPKARGHVRLPWSYVEQNVIRERFQET
                     RLFQLTSSQQSEMVTLHLLLSFNQETNALIRQLWRQVVGLQRLKNIGLGLLSTVWIMA
                     VVWGYLQLDLRTQG"
BASE COUNT          340 a          498 c          573 g          456 t
ORIGIN      
        1 tgttccgttt gagggggaga gtgtcggcga agttttgatg aaacatctga cgaagttgcc
       61 ggacctcacg ccacttccgc cggaagtccg tcgcgtggtc ggccgggcac tggaaaagga
      121 ccccgcacgt cgatttcagg atgtccgaga aatggtggcc ctcttgccag gaatggaaca
      181 gtcgccctat gttgagatgg gcactgcgaa ggcggcaggt ggcccgaccg agaaggagca
      241 gttccagtcg cctggctcag ccaagatggg tcacggagaa gaaccggtca tggccgaact
      301 ggcggaggaa tcgaatcgtc ccgaacctat ttggcacgcg gtgtctcaag cctttcggga
      361 tttgcgtacg gcctggaata attcgcgctt ggctacgcca ctaagggtgg caattctcat
      421 cggtgtcgct ttcctggtaa tctcccatgt gggaatcctc gggccggtca ttttcgggct
      481 tttgatggct tatgcggtgt attggaccgt tcgggctatc ctgattgcga ctatgggcca
      541 ggagcatgtg gttcgtggta cggctattcc tgcggaattg gtcgctgccc cggcgcaacc
      601 gacgtggccc cagcgagttg ctgccggtgc gccgccaccc gttcccgagc cttctcgggc
      661 tgaggcgttc cgtcagtata tgagccaaat gtccttccgt gcccaccttg cggaaacact
      721 ggggtcgttt ctcgcggcct ttctcatcgc gggtgtccta gctgtcgtcg gttacctggt
      781 gcagagtttc tatgggaaga cgatgacgct gacacaggca tcctgggttt ttttgatcac
      841 gatcctggga agctggacca tcttgctggt cagcaaagtg tggcaagtgt gggaaagcga
      901 tgccagcgta cggcgttttg tgatgatggt cctcggtttg gcgctgggga ttgtgggctt
      961 tggcttgacg caatacatcc aggtggactt aacatttaac gcccgctggc cgaatttcca
     1021 cgattggcaa cctcccgcgt cattttaccg agacggtcag ccgatgttgc ctgccctgat
     1081 ggccgcgttt gggacccatt ttctgctcgt gcggtggtgg cggttggccg atccgttgcg
     1141 gcgaagtcgt ttctgcctgt ggggagtcct cggaccggcc attgccgcga tcctggtgag
     1201 tgctatttgg gtatttcccg ggcaatggct cctatggcca gccgtggcta tggggatcac
     1261 agtgcaactg gctgccccgt ggttttcgat acggcggaaa cggcggtcac tcccgcagcg
     1321 ggctcatcca taggagacgc cgtcatgatg ttgagagtta acctctgggt tttgcttatc
     1381 atgtgtgccc tcgctagccg agtgttggta gccgagccgc cgtcgtgggt ggatgcccgc
     1441 cctcagcgag tgaacggcga gtatcaaatc cccattcatg ttgggcctta cgcgaccgtc
     1501 atcgagtgcg aaagtaatct ccatccgatc gtccaagcga ccattgacga ttacgttgaa
     1561 cagcttattg gcccaaaagc ccgcgggcac gtccgactcc cctggtccta cgttgagcaa
     1621 aacgtcattc gggagcgatt tcaggagacg cgacttttcc agcttacatc ttcgcaacaa
     1681 agcgagatgg taaccctcca cctgctccta agtttcaatc aagagacaaa tgcccttatc
     1741 aggcagcttt ggcggcaggt tgtcgggctg caacgattaa agaacattgg tctggggctt
     1801 ttgagcactg tctggattat ggccgttgtg tggggttatt tacagctcga tttgcgaacc
     1861 caagggc
//
LOCUS       DAOPTR010000851         1865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_48739_length_1865_cov_2.607182, whole genome shotgun sequence.
ACCESSION   DAOPTR010000851 DAOPTR010000000
VERSION     DAOPTR010000851.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48739_length_1865_cov_2.607182"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(305..643)
                     /locus_tag="PKI05_13830"
     CDS             complement(305..643)
                     /locus_tag="PKI05_13830"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOA53329.1"
                     /translation="MTGIQDQEIFGKPMNLADLLSVQPKAIVSRILVKHSGGSITLFA
                     FDAGQAVSEHTAPFDAMIHVLEGAADVTVGQDKHTVNAGESILLPANVPHAVRAPQAF
                     KMLLFMLRKE"
     gene            1165..1653
                     /locus_tag="PKI05_13835"
     CDS             1165..1653
                     /locus_tag="PKI05_13835"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOA53330.1"
                     /translation="MLSPKMLQALNDQINAELYSSYLYLAMAARCEALDLPGAAHWLR
                     AQSQEEHEHAMKFFRYVNERRGEVKLQAIKEPPCSWNKLCELFEDVLAHEQYISKRIN
                     DLVKLARDENDYATENFLQWFVKEQVEEEATVDAVIKRLRLVGEMPQGLFFIDRELGQ
                     RS"
BASE COUNT          436 a          488 c          525 g          416 t
ORIGIN      
        1 atgcgtcaga gatgcatgcg ccgttatgcg tatcggagga acacttgcga gatattgggc
       61 aaatcacgac gagttcgtaa tgtgcgcaat tagtaagata gagagacttt tcggagcacc
      121 tcccaggctt ggcgtccccc atccttttcc ttaccgggat aagggtcgtc gaaacgcctc
      181 atcctgccgt gaaatgtcat ggcctacgca gaccaaaaag ctgcgcaaaa gcagcagtcc
      241 gccggtggcc tccgagaagg ttgagggtac cagcaaaagt aagccgctca agatcgcaag
      301 cggttcactc ttttcggagc atgaagagga gcatcttaaa ggcctgcggg gccctaaccg
      361 cgtgagggac gttggccggt aacaagatcg attctccggc gttgaccgtg tgcttgtcct
      421 ggccgacggt cacatccgcg gcaccctcta ggacatggat catggcatca aaaggggctg
      481 tatgttctga cactgcctgc ccggcatcaa aggcaaagag cgtgattgat ccgccggagt
      541 gtttgacaag aatccgactg acaatggcct tcggttggac actcaacaga tcggccaaat
      601 tcatcggctt gccgaatatc tcttggtcct ggattccggt cataaaaaag tcctcgcttt
      661 ccctcggtct tcgctcttgc ggtcaactat tttaggtacg ggccaatcgc accggaaaat
      721 ctcaaaatat gctccacatc ccggccaaag cgggccgaat tctgcgaatc gtacgtggta
      781 ttatatacgg cctggaaaag cgtgctaaca gtctgatcaa actcacgagc attattgggg
      841 gaaaagggga ggcgttatcg ccgtccccaa tggcttagaa actggagcta cattttccga
      901 attcttccgc ctcccgatcc atgtgggaca ggtcgtttcc gtgcctgttg gcgctagatc
      961 tggccccgta tggtggtggt gcgaaatggc gcgtggccgt gcaaagcggg gtcccacaaa
     1021 aacggcgtgg cttgccttcc ttcgaggttt ggtaataatt catgaatttc ccgtcgttgc
     1081 agcggagggg ccaattgggt aaaaagaccc ttcgcagcat ttcccatcga ttgacaaaac
     1141 ggtgataacg gaaaggagtg tcccatgtta agccccaaga tgttgcaggc tttgaacgat
     1201 cagatcaatg ccgagctcta ttcctcgtac ctttacctgg cgatggccgc ccgctgtgaa
     1261 gcccttgacc ttcccggggc cgctcactgg ttgcgtgccc aatcccagga agagcacgag
     1321 cacgccatga aattctttcg gtacgtaaac gaacggcggg gtgaggtcaa gttgcaggcc
     1381 attaaggagc ccccgtgttc ttggaataag ctttgcgagc tctttgagga tgtcctcgct
     1441 cacgagcaat acatcagcaa gcggatcaac gatctggtta aattagctcg ggacgaaaac
     1501 gactacgcca ccgagaattt cctgcagtgg ttcgtgaaag agcaagtgga ggaagaggcc
     1561 accgtggatg ccgtcatcaa gcggctccga ctggtcggag agatgcccca ggggctgttc
     1621 ttcatcgatc gagaactggg ccagcgaagt tgagggctat cgcgttcgct cggccggtaa
     1681 aaacctgttg atctttaaca cgcaattgcc ttaacggcgc aggatcgaga tggttctgcg
     1741 ccgttttttg tttacaagcc accgcggatt cgggcaagtt gccgatccag ccgttggggc
     1801 gaaacaggga tagccgtccc caaggcctgg gcaaataggg agatgcggaa ttcttccagc
     1861 atcca
//
LOCUS       DAOPTR010000852         1864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_48759_length_1864_cov_3.715865, whole genome shotgun sequence.
ACCESSION   DAOPTR010000852 DAOPTR010000000
VERSION     DAOPTR010000852.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48759_length_1864_cov_3.715865"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..532)
                     /locus_tag="PKI05_13840"
     CDS             complement(191..532)
                     /locus_tag="PKI05_13840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOA53331.1"
                     /translation="MQLAYEIFGDIAVVHAPEELSEDSVPALFAALEQIQVRHIVLDL
                     DRTELLDSAGLTAIVNAHEMLQQKQGSLRLVASQPINRKILHITGIEQTIPVFNTVVE
                     AAKTLHGGVSE"
     gene            complement(584..970)
                     /locus_tag="PKI05_13845"
     CDS             complement(584..970)
                     /locus_tag="PKI05_13845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOA53332.1"
                     /translation="MSKQVLLCDDEIAILKAAEFKLKKAGFAVRCADNGETAWTMVCE
                     AAPDLIISDYQMPRLDGIQLLARIRNYSVTKATPFILLTGKGFELDGEELRRTLNATE
                     LLPKPFSPRALLEKVISILGQPSCGV"
     gene            complement(980..>1864)
                     /locus_tag="PKI05_13850"
     CDS             complement(980..>1864)
                     /locus_tag="PKI05_13850"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOA53333.1"
                     /translation="VFDWQTAGPEGRSYHYRVVVREIGSSENGNRLECGKDRAVFLQD
                     LTPLQELRKRHAEFVSAASHEMKAPLAAIRAYTEMLLDGEAGDEHTREEFISTIEAQA
                     QRLQRLVENLLNTARIEAGILKVHKRPISVNEILQEAVRLMTAAANEKDIALLLEPSP
                     LYLPINADRDLMLQAVINLISNAIKYTPNGGKVTVRSQLQDQTVVIEVEDTGVGMSPA
                     DVTRVFEKFYRVEAHKNMAPGTGLGLPLTKHIVEDVHGGTISVRSKVNEGSVFSIALP
                     KASAAADRARTSQELATV"
BASE COUNT          419 a          515 c          455 g          475 t
ORIGIN      
        1 tatcccaggg aaagcaaaat ttcaccaagt agcctggtgc ggccacttga acgttgctgt
       61 tctaaagcta cctgaagttg ctcgggacgc agatacccct tttcgacgag gatatcgcca
      121 agtcgccgtc gcacattcgt ttttcgcaaa tccgtcgtgg acatgtttga ttccattacc
      181 tgacgcgtgg ttattccgat accccaccgt gcaacgtctt ggctgcttca acaactgtgt
      241 tgaatacagg aatggtctgt tcaattcccg tgatatgaag gattttgcgg ttaattggtt
      301 ggctggcgac caggcggaga ctgccttgct tttgttgaag catctcgtgg gcattgacaa
      361 tggccgtcag accggcacta tccagcaact cggtcctgtc caagtcaagc acaatgtgcc
      421 tgacctgaat ctgctccaat gctgcaaaaa gggccggcac actgtcttcg cttagctcct
      481 ctggagcatg aacgaccgca atgtcaccaa aaatctcata tgccaattgc atcgtggttc
      541 cttaaacggt gttcattctc ttaggctcaa ccttgtgctc agcttacacc ccacacgagg
      601 gttggcccaa aattgagatc accttttcga gtaaagcccg gggggaaaaa ggctttggca
      661 acagctctgt tgcattgagg gttcgtctca attcttcacc atccaattcg aacccctttc
      721 ctgtcagcag gataaaggga gtggccttgg tcactgaata attccgaata cgcgccagga
      781 gttgaatccc gtccagtcgc ggcatttgat agtcactgat aatcaaatca ggagccgctt
      841 cgcacaccat ggtccatgca gtttccccgt tgtccgcaca gcggacggcg aaaccagcct
      901 ttttcaactt gaactcggct gccttcaaaa tggcaatttc gtcatcacaa agaagcacct
      961 gcttgctcat ggaatctgct cacaccgttg caagttcctg agacgtccgg gcccggtccg
     1021 ccgcagcaga ggcctttggt agtgcaatgc taaacaccga accctcgttc actttactgc
     1081 ggacagaaat ggtcccacca tgaacatctt ctacaatgtg tttcgtcagc ggcaggccaa
     1141 gacctgtccc gggcgccata ttcttatgag cctcgacacg ataaaacttc tcgaagacac
     1201 gcgttacatc ggcaggtgac atgccgacgc ccgtgtcctc cacttcaatg acgacggtct
     1261 ggtcctggag ttgactccgc acggtaactt ttccgccatt gggcgtgtat ttgattgcgt
     1321 tcgaaatcaa attgatgacg gcttgcagca tcaggtcacg atcggcattg atgggcaggt
     1381 agagcgggct aggctcaagc aatagtgcta tatccttttc atttgccgcg gcggtcatta
     1441 gacggaccgc ttcttgaagg atctcattta ccgaaatagg ccgtttgtgg accttgagaa
     1501 tccccgcctc gattcgggct gtgttaagca agttttcaac tagcctttgc aacctttggg
     1561 cttgtgcttc gatagtggag atgaactcct cacgggtgtg ttcatctcca gcctcgccgt
     1621 ccaggagcat ttcggtgtag gcccgaatag ccgctaatgg tgccttcatt tcgtggctag
     1681 cggcggacac gaactctgcg tgccgttttc gcagctcctg aagtggtgtt aaatcctgca
     1741 agaaaaccgc gcgatctttt ccacactcaa gacgatttcc gttttccgag gaaccgatct
     1801 cccggactac cacccgataa tggtaagagc ggccttctgg acctgccgtc tgccagtcaa
     1861 acac
//
LOCUS       DAOPTR010000853         1864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_48773_length_1864_cov_2.667219, whole genome shotgun sequence.
ACCESSION   DAOPTR010000853 DAOPTR010000000
VERSION     DAOPTR010000853.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48773_length_1864_cov_2.667219"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1864
                     /locus_tag="PKI05_13855"
     CDS             <1..>1864
                     /locus_tag="PKI05_13855"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOA53334.1"
                     /translation="GITARLVTTEWIAQNYTGTETGDLADRIRDFIRDSYLRHGTRWV
                     LLAGDSEIIPARGVHVTVGNISDSNLATDMYYACLDGPWNADGDNLWGEPTDGLGGAD
                     IDLVPEVFVGRAPVSSVVEARNFVTKTLTYTNVAHPNLESALLLGEKLDSITQGSTSN
                     EIIRQQAIPSNWNITTLYDGATSAWTTSQVIASLNASPNIVHHLGHANSGYVARMTTS
                     QVALLQNAFPYIMYSQGCDAGSFDTRDIAVAEQHVIAPAGAVAVIMNTRYGWYVPGAS
                     PGGSHDYALAFFKAIFNEHKTRLGEAFIESKIDNLFRLTAGGAYRWIHLSCTLFGDPE
                     LVIQTHDWQPPGNSQIAGYVYQDLNANGSFDIGEPGVAGAIVYLDLDQDGRRDQGATT
                     FTQSTPQTLVDYGTTLSRLTVSGVGKVHTLLVSVNITHTYDADLLLTLISPSGRRVRL
                     ASNVGGSGDNFVGTVFDDSAPQLIGQGVAPFTGSFRPEEPLSLFTGEDADGVWTLEVR
                     DTMYWDTGVLNDWSLTFVYDEPYVVTQADGSYVFSGLPAGTYTVCYELPSQQSLPPFA
                     NTGRTVSVARNQTVTGVNFASPSASSIINLGYVTDIIVQIGGQPQSLYRFSTRH"
BASE COUNT          406 a          521 c          490 g          447 t
ORIGIN      
        1 ggggaattac agcccgactg gtaaccaccg aatggatcgc ccaaaactac actggcacgg
       61 aaaccggcga cttggccgat cgcattcggg atttcattcg tgattcctat ttgcggcatg
      121 ggacccgttg ggtccttttg gccggtgatt ccgagatcat ccctgcccgc ggtgtgcatg
      181 tcactgtagg caacataagc gacagcaacc ttgccacaga tatgtattat gcctgcttgg
      241 atggcccatg gaatgcagac ggtgacaacc tttggggaga acctacagat ggtctgggcg
      301 gggccgacat tgacctagtc cccgaagtct ttgtggggcg tgcgccggtg agttcagttg
      361 ttgaggcacg caacttcgtg accaagaccc tcacctatac caatgtggct catccgaatc
      421 tagaaagtgc cttgctccta ggcgaaaaac tcgacagcat cactcaggga agcacttcta
      481 acgaaatcat ccggcaacag gcgatcccct cgaactggaa catcacgaca ctttatgatg
      541 gggccacttc ggcctggacg acatcgcagg ttatagcgag ccttaatgct tcaccgaata
      601 ttgtgcatca tttgggacat gctaattcgg gttacgtcgc gaggatgacg acctcgcaag
      661 ttgccctgct gcagaacgcg tttccttaca ttatgtattc gcagggctgt gacgccggtt
      721 cctttgacac tcgcgatatt gcggtggccg agcaacacgt cattgccccg gcaggtgcgg
      781 tcgccgttat tatgaacact cggtacggtt ggtatgtgcc gggagccagc cccggtggga
      841 gtcacgatta tgccttggca ttctttaagg ccatcttcaa tgaacataaa acccgcttgg
      901 gtgaagcctt tatcgaatcg aagattgata atcttttccg cctgaccgct ggtggtgcct
      961 atcgctggat tcacctcagt tgcacgctct tcggtgatcc cgaactggtt atccagaccc
     1021 atgactggca accgccaggt aactcacaga ttgccggtta cgtttaccaa gaccttaacg
     1081 ccaatggatc gttcgatatc ggagagcccg gggtggcggg cgccattgtt tatcttgacc
     1141 ttgaccagga tggccgacgc gatcagggag caacgacatt cacacaatcg acgccccaaa
     1201 ccctggtgga ctacggaaca acactctcca ggttaacggt ttctggggtg ggtaaagttc
     1261 acacactgct tgtttcggtt aatattaccc acacctacga tgccgacctg ttgttgaccc
     1321 tcatcagccc ctccggacgc cgtgtcaggc ttgcctcgaa tgtcggtggg tcaggggata
     1381 attttgtcgg cacagtcttc gatgattccg ctccccagct catcgggcaa ggagtcgcac
     1441 cgtttacggg gtccttccgg cctgaagaac ccttgtccct gttcaccggc gaagacgccg
     1501 acggtgtctg gacccttgag gtccgagaca caatgtactg ggacactggc gtcctcaatg
     1561 attggtcact aacgtttgtg tacgacgaac cttatgtcgt gacccaggcg gacggtagct
     1621 acgtcttttc tggtctgccc gcgggaacat acacggtgtg ttacgaactt ccatctcagc
     1681 aatcgttgcc gccgtttgcc aataccggcc gaacggtgag cgtggcccgc aaccaaacgg
     1741 tcaccggcgt caattttgca agtccatctg cctcatcgat tatcaatctg ggctatgtca
     1801 ctgatataat tgtacaaatt ggcgggcagc cacaatcact ctatcgtttt tccacacgcc
     1861 atga
//
LOCUS       DAOPTR010000854         1860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_48918_length_1860_cov_2.861496, whole genome shotgun sequence.
ACCESSION   DAOPTR010000854 DAOPTR010000000
VERSION     DAOPTR010000854.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48918_length_1860_cov_2.861496"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..300)
                     /locus_tag="PKI05_13860"
     CDS             complement(31..300)
                     /locus_tag="PKI05_13860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOA53335.1"
                     /translation="MCLGIPGRILDKYERNGLLYGRVDFGGIAKEICLAYVPEAKPGE
                     YVIVHVGFAISRLDESEAMEQLALLREIDAAGEAAEEPQDMEKKA"
     gene            complement(329..>1860)
                     /locus_tag="PKI05_13865"
     CDS             complement(329..>1860)
                     /locus_tag="PKI05_13865"
                     /inference="COORDINATES: protein motif:HMM:NF036810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53336.1"
                     /translation="TDRSGNVLMVRRARGFAPRSLALAGHQVPILATGGHLKNVVGLA
                     IGNQAILSAHIGDLDNPLAFEAHRRAIEDLLRFFRTTPEAITCDLHPDYGSTRSAEDL
                     ARRLKTPEFRWQHHVAHFAACLAEAESDEARRAVNELALSQETTTPGHPSGELPILGV
                     IWDGTGYGTDGTSWGGEFFLFDGTSFCRVARLRTFPLPGGDIVVRQPRRSLLGLIYET
                     GDRSLEQTLEKFFTPQELKVLLRAMERNVQSPRTSSMGRLFDGIAALLGFGGHTSFEG
                     QAAMALQYAAERGLLRAYPIISPRPFGGRGDGGEGGSNRCEFSQSIPTFLCPHPNPLP
                     EGEGDFLRQALNSRRPPPKTAGKPSECDPEKQADVLATAHEATDSTAVEPLLVHEVNG
                     LLELNWQPLFQSVATAMSRGMSRELLALAFHVRLVDSVFQLYQMLRCRSLVISGGCFQ
                     NQLLRELIGAEANRFGVPLYQPQLIPAGDGGIALGQLWLTARTLQGLVRADSLCEYIR
                     P"
BASE COUNT          405 a          549 c          516 g          390 t
ORIGIN      
        1 ctgtgctgtc acgatactcg gaaatgaacc tcacgccttt ttctccatat cctgcggttc
       61 ctcggcagcc tcgccggcgg cgtcgatctc cctgaggagg gccagttgtt ccattgcctc
      121 ggattcatca agccgactaa tggcgaatcc gacatggaca atgacgtact ctcccggttt
      181 ggcttccggg acgtacgcga ggcagatctc tttggcgatt ccgccaaaat cgacacgccc
      241 ataaaggaga ccgttccgtt cgtatttatc aaggatcctt cctggaatgc ccaaacacat
      301 gggttcgtct cactttcttt aagttatctt aaggtcgaat atactcgcac aatgaatcgg
      361 cacggaccaa gccctgaagc gttcgggccg ttaaccatag ttgtcccaaa gcgatccccc
      421 catctcctgc cgggatgagc tgcggttggt acaacggaac gccaaagcga ttggcctctg
      481 ccccgatcaa ttcccgcaat aattgattct gaaagcagcc gcccgagatc accagggacc
      541 tacaccgaag catttggtac aactgaaaaa ccgagtcaac caggcgaaca tgaaaggcta
      601 gggccaatag ttcccggctc atcccgcggg acatcgccgt ggccaccgat tggaacaaag
      661 gttgccagtt caactcgagc agaccgttga cctcgtgcac tagcagtggt tcaaccgccg
      721 tggaatccgt cgcttcgtgt gctgtagcaa gcacgtccgc ttgcttctca gggtcacatt
      781 cgctgggctt gcccgcggtt ttagggggag gacgtcggct attaagagcc tgtctcaaaa
      841 agtccccctc tccctccggg agagggttgg ggtgagggca aaggaacgtt ggaatcgatt
      901 gggaaaactc gcagcgattc gagccaccct cacccccgtc ccctctcccg ccgaacgggc
      961 gaggggagat tatgggatag gctctaagta ggcccctttc tgcggcgtat tgaagggcca
     1021 tggctgcttg gccttcgaaa ctcgtgtggc ctccaaatcc cagaagggcg gcgattccgt
     1081 cgaacaatcg gcccatactg gaggtgcgcg gggattgcac atttcgttcc atcgcccgca
     1141 gcaggacctt caactcttga ggggtgaaga acttctccag cgtctgttcc agagaacggt
     1201 cccctgtttc atagatcagg ccgagaagtg accggcgcgg ctgccggacc acaatatcgc
     1261 ctcctggcag ggggaatgtc cgcaatcggg cgacgcggca aaatgatgtt ccatcgaaca
     1321 aaaagaactc cccaccccag ctcgtcccgt cggtgccgta acctgttccg tcccagataa
     1381 cgcccaagat gggcaattct ccagatggat gtcccggtgt tgtcgtttct tgggacaagg
     1441 ccaactcatt gacggcacga cgtgcttcgt cgctttcggc ctcggccaga cacgcggcga
     1501 aatgggctac gtggtgttgc cagcgaaact cgggggtttt caggcggcga gccaaatctt
     1561 ccgcactgcg ggtcgatccg taatccggat ggagatcaca tgtgatcgcc tcgggcgtgg
     1621 tccggaaaaa gcggagcaaa tcctcgatcg cgcggcgatg agcctcaaag gccaagggat
     1681 tgtccaggtc cccaatatgt gcgctcagga tagcctgatt tccaatggcc agtccaacca
     1741 cgtttttcaa atgtccgccc gtagcaagga tggggacttg gtgacccgcc agagcaagtg
     1801 atcgcggagc gaagccccga gcacgtcgga ccattaacac gtttcccgat cgatccgtgg
//
LOCUS       DAOPTR010000855         1859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_48955_length_1859_cov_3.111973, whole genome shotgun sequence.
ACCESSION   DAOPTR010000855 DAOPTR010000000
VERSION     DAOPTR010000855.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48955_length_1859_cov_3.111973"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="PKI05_13870"
     CDS             complement(<1..632)
                     /locus_tag="PKI05_13870"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOA53337.1"
                     /translation="MASEAQIQEWIHRLAAAGLSCDLPKMSEEVSQITSFAQFLEYLV
                     RQKVLTAWQAEELVKGRQSFLLGKYRLLTELGRGGMAHVFLAEHVTMERRVAIKLISS
                     NLPPSARDRFLAEVRTIASLDHPNIVHAYNVDCDGAQYYLVMEYVEGKTLEHMVRESG
                     PLPWRQATDIARQTALGLGYAHAQGVIHCDLKPANIIVNSEGVVKILDLGL"
     gene            complement(655..1329)
                     /locus_tag="PKI05_13875"
     CDS             complement(655..1329)
                     /locus_tag="PKI05_13875"
                     /inference="COORDINATES: protein motif:HMM:NF012624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOA53338.1"
                     /translation="MTPSSSRTGVILLGPTGAGKTPLGESLSAWGHGPWRFHHFDFGH
                     WLRIVISDQESQCLFDDEERHLVADILASGRLLKDEEFFIADRLLQSFLSALPHESDR
                     QEVIILNGLPRTLFQAERIIRLLPIQLVVNLQCTLDVVFERIHSNVGGDRTGRDDDLE
                     SLVAKKYCLYESETRPLVDYYRGLGIPVVSLAVTTTTTAEELRDQFLDRWSKLFEGTR
                     SKTSMC"
BASE COUNT          453 a          492 c          481 g          433 t
ORIGIN      
        1 aggccgaggt caagaatctt aacgactccc tccgaattga cgatgatgtt ggccggtttt
       61 aggtcgcaat ggataacgcc ttgagcatgg gcatagccta agcccaacgc agtttgtctt
      121 gcgatgtcag tcgcttgcct ccagggcagg ggacccgact cgcggaccat gtgctcaagc
      181 gtcttaccct cgacatactc catcaccaag tagtactggg ccccatcaca atcaacgttg
      241 taggcatgaa caatgttagg atggtccagc gacgcaattg tccgaacctc ggcaagaaaa
      301 cgatctctgg ccgatggcgg caaattcgag gagatgagct tgatcgccac acggcgctcc
      361 atggtgacat gttccgcgag gaagacgtgg gccattcccc ctcggcctaa ttccgtgagc
      421 agacggtact ttccaaggag aaaactttgg cgtcctttga ctaattcctc cgcttgccag
      481 gcggtaagga ccttttgacg gactagatac tccagaaact gcgcaaaact ggtgatttgc
      541 gagacctcct cgctcatttt cggcagatcg cacgacaagc cagcggcggc gagccggtgg
      601 atccattcct gaatttgtgc ttcggaggcc atattcgccc tgcctcaaaa ctcatcaaca
      661 cattgatgtc tttgatcgcg ttccctcaaa taatttgctc catcggtcga ggaactgatc
      721 tcgcagttct tcggccgtcg tggtcgttgt aactgccagg gaaacgacgg ggatcccaag
      781 acctcgatag taatcaacaa gaggccgcgt ctctgactcg tacaagcaat actttttggc
      841 caccagggat tctaagtcgt catctcggcc agtacgatcc cctcccacat tggagtggat
      901 ccgttcgaag accacgtcca aagtacattg aaggttgacc acaagttgaa ttggcagaag
      961 ccttatgatc ctttcagctt ggaacagcgt acggggtaac ccgttgagaa tgataacttc
     1021 ctgacgatcc gattcgtgcg gaagagctga caaaaaagac tgtaacaaac gatctgcgat
     1081 gaagaattcc tcatctttga gcagacgccc agaggcgagg atgtccgcaa cgaggtgtct
     1141 ctcctcgtcg tcaaaaagac attgcgactc ttgatctgaa atgactatac gaagccaatg
     1201 tccgaagtca aaatgatgaa atcgccaggg cccgtgtccc caagccgaaa gagactcgcc
     1261 aagcggggtc ttgccggctc cggtaggccc aagaaggatc acacctgtgc gagaagatga
     1321 tggcgtcatt gtggtactaa acaatctgtc gttaacatgg gaactggaca ttcctattct
     1381 aagacttacc cgcgtggaaa gcacctttgg ttaccccaca atgtcaggtt gagaaacgtc
     1441 tgtgggggac gtgaaaacgg caatcgaatt taaagtcctc gggcggccta ttttgcgact
     1501 cggttgactc ctgggatgtc gcgattccat ctgtaggatc cttcacgggc agcgacatca
     1561 tgggtatgat ccgtctatcg ccttggaaca cggcaatgct gcttgtttat gttgatgcgt
     1621 ctttttcgca ctactgagaa agcgccgcta ctattggcat tgtggtgatt gtcaggcgaa
     1681 gcatgccctt tgccccgatg acaagtcggc actgcacaca tgaagttcaa aatagcattt
     1741 gccataactg aattggaaat gggcggggcc gaaagggccc tcaccgagct ggtacttcga
     1801 attgatcggg caagatttga gccctgcgtc ttcgtcctct cggtaccccc gctcgacca
//
LOCUS       DAOPTR010000856         1858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_48997_length_1858_cov_2.440377, whole genome shotgun sequence.
ACCESSION   DAOPTR010000856 DAOPTR010000000
VERSION     DAOPTR010000856.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48997_length_1858_cov_2.440377"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(403..1566)
                     /locus_tag="PKI05_13880"
     CDS             complement(403..1566)
                     /locus_tag="PKI05_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53339.1"
                     /translation="MGVYHQMGHDSWNLVGEKPLNGFAGMILSPVNDTPDEVIQKISL
                     IKSPPKKFEIILDPQFYEPRTARGHLSDWSHFSEDVDTVDLGDLKWWEGRATALVSVA
                     KTIGAHAICSPAFCPKNNAYDDSYFSWVVECANRANIHAADAKLDVLLTAIVGLSELA
                     KKGRPQAIASRLTATDISRVYLVFADDLPPRTQRTNVEALGGAVLLIRLLEEAGSQVL
                     VAFSGLDMLLWKAAGATNVASGKFFNLRRFVPGRFQKDAQEGGRVVPYWTDGPLVTWL
                     REDDVRMLDRRGIISRKASVTNPYSQRILDILDAGEGKPWVGEGWRQYLYWLQETESN
                     VSGDIARILSLLKAADSAWGAIATAEIYLYDRQNTGEWIRPWLNAIKIGLEDE"
     gene            complement(1567..>1858)
                     /locus_tag="PKI05_13885"
     CDS             complement(1567..>1858)
                     /locus_tag="PKI05_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53340.1"
                     /translation="EQLHNEKCAIDWRTDQFGLALVLAELLLGHHAFLEDPKGNVNDA
                     ILRVASRRPLPEGSRQRLLESGFTCLVKALAIWPVQRYRKPDDFARAIAEEA"
BASE COUNT          447 a          527 c          457 g          427 t
ORIGIN      
        1 ctctccgatc gatatgacat gtctatctcc ctgctgattc tcgacacgcc aggagtggaa
       61 catccagacg cgccggacga agacatgacg actacaccac ccacgttttg aaaaccgacg
      121 caagcaaagg ccacgaggag gtttcatcac tacgcctcgc ctgacctttg cgttttcgcg
      181 gatgacacca agtcaccgat agaggcaagg acggctttta cctcaaagcg gcggaatggt
      241 gctcgaagaa atggtccgct tccgatcggc cagaagcatt atgatccttc actcggagtg
      301 aaggcggtag ggagcggacc gggcatgccc actgtttagg cgggcagccg tcgttccggg
      361 gatgtacagg cgagtgttgc acatgacaga gcgcttgcaa ctttactcgt cctccaagcc
      421 gatcttgatt gcgttcaacc acggacgaat ccactctcca gtgttttgac ggtcatatag
      481 atatatttcg gcagtggcga tggcccccca cgctgagtct gccgctttca gcaaagatag
      541 aattctcgcg atgtcgccgg aaacgttcga ttctgtttct tgaagccaat aaaggtattg
      601 ccgccatcct tcgccaaccc atggcttgcc ttcgccagca tcaagaatgt cgaggatccg
      661 ctgcgagtag ggattggtga cggaagcttt acgggagatt atccctcttc tatccagcat
      721 cctgacatcg tcttcacgta gccaagtgac aagtgggccg tctgtccagt atggcaccac
      781 ccgcccacct tcttgggcat ctttttgaaa acgcccggga acaaatcggc gcaggttgaa
      841 aaactttccg ctcgctacgt tggttgcgcc ggctgccttc cataaaagca tatccaagcc
      901 actaaacgcg accagcacct ggctccctgc ctcttcaagt agtcggatca agagaacagc
      961 accacctagt gcttcgacgt tcgtccgttg agtgcgggga ggtagatcat ccgcgaagac
     1021 cagataaaca cgagaaatat ccgtagccgt aagacgtgat gcaatcgcct ggggtcgccc
     1081 tttcttggct agttctgaca gaccaacaat tgcggtcagc aaaacatcca acttggcatc
     1141 ggctgcgtgt atgttcgccc gattggcgca ttcgacaacc caactgaaat agctatcgtc
     1201 ataggcattg ttcttcgggc aaaaagccgg agagcagatg gcatgagcgc caatggtttt
     1261 ggctaccgaa acaagcgccg tcgcccggcc ctcccaccat ttgaggtcgc ccagatcaac
     1321 cgtgtcgaca tcctccgaga aatgtgacca atccgataga tgccctcgtg cagtacgcgg
     1381 ttcataaaac tgcggatcta aaataatctc aaattttttc ggcggactct ttattaaaga
     1441 gattttctga ataacttcat caggagtgtc gttgactggg ctcaaaatca taccggcaaa
     1501 cccatttagc ggtttctcac cgacgagatt ccaggagtcg tgccccattt ggtgataaac
     1561 gcccatttat gcctcctcag caatagcacg cgcaaaatca tccggcttac gataacgttg
     1621 taccggccat attgctaacg cctttaccaa acatgtgaac ccagattcca gcagtcgctg
     1681 ccgtgatccc tccggcaggg gcctacggct ggcaactcgt agaattgcat cattcacatt
     1741 ccccttcgga tcttcgagga aggcgtgatg ccccaacagc aactcagcta gcaccaaggc
     1801 tagcccgaat tggtctgtgc gccaatcgat agcgcatttc tcattgtgga gttgttcg
//
LOCUS       DAOPTR010000857         1852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_49212_length_1852_cov_4.052866, whole genome shotgun sequence.
ACCESSION   DAOPTR010000857 DAOPTR010000000
VERSION     DAOPTR010000857.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49212_length_1852_cov_4.052866"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PKI05_13890"
     CDS             complement(<1..501)
                     /locus_tag="PKI05_13890"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOA53341.1"
                     /translation="MKSILRWSIRNAPAMNTLLIGLLVVGTVSLLRMRREVFPDFNLD
                     MILISVPYPGASPTEVEEGICQKIEEAVRAVAGIKKTYSVARESGGAVILELFSNADA
                     QRVLDEVRSEVDRIPSFPEMAENPEIKLITIRRAAITVGVIGPDSDDPRSERALRELA
                     EQVRDEL"
     gene            complement(498..>1852)
                     /locus_tag="PKI05_13895"
     CDS             complement(498..>1852)
                     /locus_tag="PKI05_13895"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOA53342.1"
                     /translation="VVIGLGILGFLTLKALKKPVPREKPQSPLPLVQTTRVVRHEGGL
                     ELKTDGVVVPYREIQIATEVDGRIVEKTADCEAGRYVTKGTLLLTIDKSDYQIEYARL
                     QEQLRQAEASLAELDVEINNTQRLLEVAEQQFEIQKQDFARAERLFGTKAISQAEFDA
                     ARKSLLLARQELINLENLLRLKQSSKERLVAARNQVEVSLKKAELDLSRTEIRAPSDG
                     VVISDLVEQNSYVRKGTVVVTFEDTSVAQVRCNLTTRQVAWLWALIHGKTLENADRGE
                     AYQVPKLPVTVEYNLDGRVFVWNGVLDGYEGIGVDDRTRMVPCRVLVDRPTVVSIKAG
                     EGKSKESLVDVPRHPPALVRGMYVTVRIYVAPGVPVLRVPERAVQPGGMVGRIRDGKL
                     KMIPVELAEVVPEGALIVAPPDILQEGDSVAVSPTPFIENNPLAISGDGLPVEEQPVL
                     "
BASE COUNT          415 a          579 c          439 g          419 t
ORIGIN      
        1 cagctcatcc cgcacttgtt cggccaactc acgaagggcc cgttctgacc gaggatcgtc
       61 ggagtccggt ccaatcaccc ccacggtaat cgctgcacgg cggatggtga taagtttgat
      121 ctccggattc tcggccattt ccggaaaact cggaatgcga tctacctccg aacggacttc
      181 atccaagacg cgctgggcat cggcattgct gaatagctcc aagatgacgg caccaccact
      241 ttcccttgcc acggaatagg tcttcttgat ccccgccacc gcccgaacgg cttcctcgat
      301 tttctggcaa attccctctt ctacctccgt ggggcttgcc cctggatagg ggacactaat
      361 caggatcatg tcgaggttaa aatcggggaa gacctctcgc cgcattcgga gaagactcac
      421 cgtcccgacc acaagcagcc caatgagcag cgtgttcatg gccggggcgt tgcgaatgga
      481 ccaacggaga atgctcttca taagaccggc tgctcctcaa ctggcagccc atctcctgaa
      541 atcgccaagg gattgttttc aataaacggc gtcggcgaaa ccgccacact atcgccttct
      601 tgtaagatat cgggtggggc aacgatcaat gccccctcag gaacaacttc cgcaagttcc
      661 accgggatca tcttcaactt gccgtcacga atccgtccca ccatcccccc aggttggaca
      721 gcacgttcgg gaacgcgaag caccggcact ccgggagcga cgtaaatcct taccgtgaca
      781 tacattccgc gaacaagggc cgggggatgt cgcggaacat caactaggga ctctttggat
      841 ttcccttcgc cggcttttat tgagacaacg gtggggcgat ctacaagaac tcggcaagga
      901 accatgcggg tgcgatcatc cacgccgatt ccttcatatc catctagcac accattccaa
      961 acgaacacac gcccatcgag gttgtactca accgttaccg gcaattttgg aacctgataa
     1021 gcttcccccc ggtccgcgtt ctccaacgtc ttcccgtgga tcaaagccca aagccatgcg
     1081 acttgccgag tcgttagatt acatctcacc tgggccacag acgtgtcttc aaaggtcacc
     1141 accactgtgc cctttcgcac gtagctattt tgttcgacaa gatcactgat cacgacaccg
     1201 tcgctgggtg cgcggatctc cgtccgggaa agatccaact ccgccttttt caaagatacc
     1261 tcaacctgat tccgcgcagc gactaaacgc tccttgctac tctgtttaag gcgcaggagg
     1321 ttctcgagat tgatcaattc ctgcctggcg agaagcaggc tcttgcgcgc cgcatcaaac
     1381 tccgcctgac taatcgcctt tgtgccaaac aaccgttctg cgcgggcaaa atcctgcttt
     1441 tggatctcaa actgttgctc cgccacttcc aggagccgct gcgtgttatt aatttcaaca
     1501 tccagctcag ccaaactggc ctctgcctgt ctcaactgtt cctgaagcct ggcatattca
     1561 atctgataat cacttttgtc gatcgttaac aacaatgtgc ccttggtcac gtatcgccct
     1621 gcttcgcagt cagcggtttt ttccacaatt cgcccatcca cttccgtcgc gatctgaatt
     1681 tcgcggtagg gaacgacgac gccgtcggtt tttagctcga ggccaccctc atgtcgaaca
     1741 acccgtgtcg tctgaacgag cggcaacggc gactgaggtt tttcacgggg cacgggcttc
     1801 ttcaaggcct tcagggttaa gaagccgaga atccccaggc caatgacaac aa
//
LOCUS       DAOPTR010000858         1849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_49324_length_1849_cov_3.527313, whole genome shotgun sequence.
ACCESSION   DAOPTR010000858 DAOPTR010000000
VERSION     DAOPTR010000858.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49324_length_1849_cov_3.527313"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            542..>1849
                     /gene="hisD"
                     /locus_tag="PKI05_13900"
     CDS             542..>1849
                     /gene="hisD"
                     /locus_tag="PKI05_13900"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008655020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOA53343.1"
                     /translation="MLNIRWVDCRTEQWRKVFDELRARLSLRGDIVTPESQEKTVKIF
                     GEPLTPAQVVERICHDIRTRGIEAAIEYTRKIDAVELTPETVRVPVDRIKAAHEQADP
                     AFLQCIRRIRSNIEEFQRAILHHDVRVTRPGGYLLERYLPLTRVGVCVPGGAAAYPST
                     VLMTVIPALVAGVREIAVIAPPTRFGAFNPDILATCAELGVWEVYSLGGAQGVAALAY
                     GTEVTPRVDKIVGPGNLFVALAKKYVFGEVDIDSIAGPSEVAIIVDDTANPTFIAFDL
                     IAQSEHSPGVSVLIGWDEDVLRRIVMTVEECLEKVDRGPLARRCLEDFGAVVLVKDQD
                     QACQVADELAVEHLQISTRNAEALAQRIPHAGAIFLGHYTPVALGDYAAGPSHVLPTG
                     GTARFASALSANDFLRATSILCFDQSGLEHLADDVAYLATKEGL"
BASE COUNT          417 a          500 c          532 g          400 t
ORIGIN      
        1 tagcaaccat gctcccacac ttgggacacg gcaaaatggc gcccgctgcc ttcgccgagc
       61 caaccctcaa gcgagccaag caggtggggc actgaatgac aaattctgca ctcacgaatg
      121 cgtcttctct gacaaatcgt cgctaaggat actgcgcaag tgcgggccca ctcggtcacc
      181 ctacgccggt acgccttcaa cccgccggca tggtacccga ggttaccctg aattgatcgt
      241 taccaaaaag ccaccgtttc ttgccgcttc ttatgagtca cttacgcact attgcgactt
      301 attgtaccac cataaagcgg actagttacc gcacaccgct gctgccaggc cactcgtcca
      361 acggccaatt ccgaacccaa tgtttgggaa atctgtcaac aacagatgtc acccaatgac
      421 caggccgatg ccaggcgcat actttccgcc acttggagta acggctttcc caagctacct
      481 tgataaatat tggttactct cagtcgcgtt gccaggtcct ttttatggtg ggatgccgag
      541 aatgcttaat atccgttggg tcgattgtcg caccgagcag tggcgaaagg tgtttgacga
      601 actccgcgcg aggctgagtc tccgtggaga cattgtcacg ccagaatcgc aggagaagac
      661 ggtcaagatt ttcggggagc ctttgacgcc agctcaagtc gtggaaagga tatgccacga
      721 tatccggacc cgcggaattg aggcggcgat cgaatacacc aggaagattg acgcggtgga
      781 gttgaccccg gagacggtca gggtcccggt ggacaggatc aaggcggcac acgaacaggc
      841 agatcccgcg tttctccagt gcatccgacg aattcgcagc aatatcgagg aatttcagcg
      901 ggcaattttg caccacgacg tccgggtcac tcgaccaggt ggctacctgc tggagcggta
      961 tcttcccctg acaagggtgg gggtttgtgt gcctggaggg gcggcggcct acccatcgac
     1021 agttttgatg acagtcatac cggcactggt ggccggcgtg cgggaaattg ccgtaatagc
     1081 cccaccgacc cgatttgggg cgttcaatcc cgacattttg gcaacatgcg ccgaacttgg
     1141 tgtttgggag gtctattcgc tgggcggggc acaaggtgtt gcagccctgg cctatgggac
     1201 ggaggtaaca ccgcgagttg acaaaattgt cgggccaggc aatttatttg tggccctggc
     1261 aaagaagtat gtttttggtg aggtcgatat cgactccatc gccggaccaa gtgaagtcgc
     1321 cattatcgtc gatgacacag ccaaccccac atttatcgca ttcgatctca tcgcccaatc
     1381 agaacactca ccgggcgtga gtgtcttgat tggttgggat gaggacgtgc tacgcaggat
     1441 cgtcatgaca gtcgaagagt gtttggaaaa ggtggatcgg ggccccctgg cgagacggtg
     1501 cctggaggat ttcggcgcag tagtgttggt caaggatcag gatcaagcgt gccaggtggc
     1561 cgacgagtta gcggttgagc acctacaaat ctccacccgc aatgctgaag cgttggccca
     1621 gagaataccc cacgcgggtg caatcttttt aggacactac acccccgtgg ccttggggga
     1681 ttacgcggca gggccgtcac atgtgctacc gacaggcggg accgcgagat ttgccagtgc
     1741 cttgtcggct aatgatttcc tcagggcaac aagtatcctt tgtttcgatc agtccggact
     1801 ggaacattta gcagatgatg tggcctatct agcgaccaag gaagggtta
//
LOCUS       DAOPTR010000859         1846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_49418_length_1846_cov_6.359017, whole genome shotgun sequence.
ACCESSION   DAOPTR010000859 DAOPTR010000000
VERSION     DAOPTR010000859.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49418_length_1846_cov_6.359017"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PKI05_13905"
     CDS             <1..861
                     /locus_tag="PKI05_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53344.1"
                     /translation="CGVSPFKSAWDVWAEKIGIAAPQPPNEAMRLGAILEPALLDWAH
                     REIAPLKRNIQRVVRLSKECRLRATLDAVFRMYGRIEPLEVKVGTAYSPRSREWGASG
                     SDEVPLDVKLQVQAQLMACQADTGHVLALLPGPRLAHYSVRADHTLQEEIKDRVIDFW
                     KYVEKKEPPPDSMPSAAIFHVIDRSGDLAPVPAEQLEEILNAYQNARAERLKAEKAEQ
                     AAKNRLFELAGAACGLMADKGDGSKLCVRFVKRTQLRLDSDRVKAMIGDDEFVKCKRE
                     VSWYEIKVEG"
     gene            864..>1846
                     /locus_tag="PKI05_13910"
     CDS             864..>1846
                     /locus_tag="PKI05_13910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HOA53345.1"
                     /translation="MNTAISQRFKDKTGSLDKATVIQNSLAAAKAQIAANAALGIDPD
                     RLIAIVASAVIRTPALLSCTPQSIVEATIKAARYGLDPSGITGDGFLVPFKDRAEFIC
                     GYRGLVKLAMRHPRVKLVEARLVHANDKFEIDYGRENPIRHKIALKDRGEPIGCYARA
                     FLDGSPPAVEWMDADEINRVRNQSKSYQKFPNDSPWTHHWGEMARKTVLRRMIKFLPL
                     EFEISLAVADLDAVDYPQFAAPAVNVLSGNGAKPIAEEKPEKPKAEAKAKKAEAPAED
                     VEVDADAGPYRVYAEKIKAAADLEAVTAIAEGIKQDDTLSQWEVDELLDLAR"
BASE COUNT          397 a          468 c          623 g          358 t
ORIGIN      
        1 tgcggcgtct cgccgttcaa gtcggcgtgg gatgtttggg ccgagaagat cggcatcgcg
       61 gcaccgcagc cgccgaacga ggccatgcgg cttggggcga tcctggaacc cgccctgttg
      121 gattgggcgc atcgcgaaat cgcacctctc aaacggaata tccagcgggt ggtgcggtta
      181 tcgaaagagt gtcgcttaag ggcgacgctt gatgccgtct ttcggatgta tggtcgcatc
      241 gagcctcttg aagtcaaggt tgggacggcc tattcgccgc gttcgcggga atggggcgcg
      301 tcgggaagcg atgaagtccc cctggatgtg aaactgcaag tgcaggcgca gctgatggcc
      361 tgccaggccg ataccgggca cgtcttggct ttattgcccg gtcctcgtct ggcccattat
      421 tcggttcggg ctgatcacac gcttcaggag gagatcaagg accgcgtcat cgacttctgg
      481 aaatacgtcg agaagaagga acctcccccg gattctatgc cgagtgcggc gatctttcac
      541 gtgatcgacc gcagcggcga cttggcccca gttccggcgg agcagcttga agagattctg
      601 aacgcatacc agaacgcgcg ggccgagcga ttgaaggctg aaaaagcgga gcaggcggcg
      661 aaaaacaggc tctttgagct tgcgggagcc gcctgcgggc tgatggccga caagggggat
      721 ggcagcaagc tctgtgtccg ttttgtgaaa cggacgcaat tgcgactgga ctcagacagg
      781 gtcaaggcca tgatcgggga cgacgagttc gtgaagtgca agagagaggt gagttggtac
      841 gagatcaagg tggagggctg attatgaaca cggcgattag tcagagattc aaggacaaaa
      901 cgggaagtct ggataaggcg acggtcattc agaattccct tgctgcggcc aaggcgcaaa
      961 ttgcggcaaa tgctgcactt gggattgatc ctgaccgcct gattgcgatt gtggcctcgg
     1021 cggtgattcg taccccggcc cttcttagtt gcacgccgca atcgattgtg gaggcgacga
     1081 tcaaggcggc acggtatggg cttgatccaa gcggcattac gggagacggc ttcctggtcc
     1141 cgttcaagga tcgggccgag tttatctgcg ggtaccgggg tttagtgaaa ttggctatgc
     1201 ggcacccgcg agtaaaactt gtcgaggccc ggctggtgca cgccaacgat aagtttgaga
     1261 tcgactacgg acgggagaat ccgattcgcc acaagattgc gttgaaggat cggggggagc
     1321 cgattggctg ctacgcccgg gcgtttctgg atggatcgcc tcccgcggtt gagtggatgg
     1381 atgctgacga gatcaatcgg gttcgcaatc agagtaagag ctaccagaaa ttccccaacg
     1441 attctccctg gacgcatcac tggggcgaga tggcccgcaa gacggtcctg cggcgaatga
     1501 tcaagtttct cccactggag tttgaaatca gtctcgcggt ggccgacttg gacgcggtgg
     1561 attatccaca gtttgcggct ccggccgtga atgtgctgag cggcaacggg gccaagccaa
     1621 tcgccgagga aaagccggag aagccgaagg ccgaggccaa ggctaagaag gccgaagctc
     1681 cggctgaaga cgtagaggtc gatgcggacg cggggccgta tcgggtctat gcggagaaaa
     1741 tcaaggcggc cgccgacctc gaggcggtga ctgccatagc tgaagggatc aagcaggacg
     1801 acaccctctc ccagtgggaa gttgatgagt tgcttgacct ggcacg
//
LOCUS       DAOPTR010000860         1846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_49440_length_1846_cov_2.522613, whole genome shotgun sequence.
ACCESSION   DAOPTR010000860 DAOPTR010000000
VERSION     DAOPTR010000860.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49440_length_1846_cov_2.522613"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PKI05_13915"
     CDS             <1..383
                     /locus_tag="PKI05_13915"
                     /inference="COORDINATES: protein motif:HMM:NF024106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HOA53346.1"
                     /translation="PLGRLAEDGKIPDCYQLRETHSADYAVRTEWNVRDSDATLILTK
                     GELSGGTRLTAIFALRYSKPLRVVNLEDNLPPKSVVDWLLSHNVRTLNVAGPRESTCP
                     EIYQMAKAYLCDLLQEWQRRCSQA"
     gene            complement(438..1229)
                     /locus_tag="PKI05_13920"
     CDS             complement(438..1229)
                     /locus_tag="PKI05_13920"
                     /inference="COORDINATES: protein motif:HMM:NF019240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1571 domain-containing protein"
                     /protein_id="HOA53347.1"
                     /translation="MTPTQSGQSDSQLRVADRVVPQTNPVVEHPLMPAIRWAYQGLQD
                     LQKIQDYSAIMVKRERVNGKLNDPEYAFVKIRHKPFSVYMHFLEPASVKGQEVIYVEG
                     QNDGKMWAHPAGIKNKLIGTVSLDPTGPIAMQNNRYPITEIGILNLVRRLIEVGEQDM
                     KYGECEVKFFEGAKINDRTCTCIQVVHPVPRRNFRFHLARIYVDTEHNLPVRYESYDW
                     PTEPDGAPQLIEEYTYLNIKVNNGFTDLDFDIRNPNYAFRVKENQ"
BASE COUNT          450 a          506 c          461 g          429 t
ORIGIN      
        1 gccccttggg acgactggcc gaggatggga aaatccccga ctgctatcag ttacgagaaa
       61 cccattccgc cgattacgca gtccgaacgg aatggaacgt ccgggacagc gacgccacgc
      121 tgatcctcac taaaggcgaa cttagcggcg gcacgcgact gacagccatc ttcgccttgc
      181 ggtattctaa acccctgcga gttgtgaatc ttgaagacaa tcttccgccg aaaagcgttg
      241 tcgactggct actctcccac aacgtgcgaa cactcaacgt ggctggcccc cgggaaagta
      301 cgtgtcctga aatctaccaa atggcaaagg catatctttg cgatttgctc caggagtggc
      361 aacgccggtg ttcccaggct taattcgtgc gcggattagc acggatagtt gctgaatttc
      421 cccaactctc gctgccgcta ctgattctct ttcacgcgga aggcatagtt tggattgcgg
      481 atatcaaaat ccagatcggt gaatccatta ttcaccttga tgttaaggta agtgtactct
      541 tcgatgagct ggggtgcacc atcgggctcg gtaggccagt cgtaggactc gtacctgacc
      601 ggcaggttgt gctcggtatc aacgtagatt cgggcaagat gaaatcggaa attgcggcgt
      661 ggcaccgggt gaacgacctg gatacacgtg cacgttcggt cgttgatctt cgctccttca
      721 aagaacttga cctcgcactc gccatatttc atgtcctgct cgccaacctc aatcagccgt
      781 cgaacgaggt tgaggatccc gatttcggta atcggatacc gattgttctg catcgcaata
      841 ggaccagtgg gatccagcga aacggttccg atgagtttgt ttttgatccc ggcgggatgc
      901 gcccacatct tgccatcgtt ttgaccttca acgtagataa cctcttgccc cttgacggaa
      961 gccggctcaa ggaagtgcat gtaaacactg aagggtttat gccgaatctt cacaaaggca
     1021 tactccggat cgttcagttt cccgttgacc cgctcacgct tgaccatgat cgcagagtag
     1081 tcttgaatct tttgcagatc ctgcagccct tgataggccc aacggatcgc cggcatcaag
     1141 ggatgctcga cgaccgggtt ggtttgaggt accacacggt cggcgactct caactgcgag
     1201 tcactctggc cactttgagt aggcgtcatt ggcgattgac caaaagtggt ccggccagaa
     1261 gcagtccaga gaaggctgag acagctgacg agactgtaaa ccatgacggc tgacacaatt
     1321 cgtcgagaaa aggatgaaat acgttgtacc atcgttattc ccctaaataa cacaaaaaac
     1381 aagttatttc cttgacgcca ctgcgggctt gcctaacaca atctccgtcc gaaattccct
     1441 gccgcgacca acctctcaag gacacctccg agtaagaggt atctatctag gtgccatcca
     1501 tctggcgcta caacccgcta ttattgccgt atcgctgatt aacatccgga acattaaaat
     1561 gatcgttctt agcgaaacgc attccgcagg aggatgcagc cccctaaccg gcattaattc
     1621 ctccgtcagc aatcacgacg tattcactct aaattacagg agaactcatg gcggacagaa
     1681 cccacccccg cgtatggacg atcgtctcag caatgttcga ccaaaattgc tacgtcgtgg
     1741 caaatgacga ttgccaagag tgcgtcatta tcgacccggg tttggagccg ggaaagatca
     1801 ttcgttcgat attggcgcaa agtttaacgc cccgagccat tctggt
//
LOCUS       DAOPTR010000861         1845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_49483_length_1845_cov_2.721788, whole genome shotgun sequence.
ACCESSION   DAOPTR010000861 DAOPTR010000000
VERSION     DAOPTR010000861.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49483_length_1845_cov_2.721788"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(407..1351)
                     /locus_tag="PKI05_13925"
     CDS             complement(407..1351)
                     /locus_tag="PKI05_13925"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HOA53348.1"
                     /translation="MLSFLVFVILVSGCGWDSSPPVHDRLGAETDHQAGGDMSPRRIV
                     SMAPSITETVFALGCGDRLVGVTDFCSFPPEAKAIPKIGGYLNPNWEAILRLQPDLVI
                     VPAGQQDFSEKLARFRLKSVTVDHRSVDGVFDSLEILGRVFGVEDRAKALASEWRQRL
                     AHLEERWKGKPRPRVLFVIERSIEPGRLRNICAAGPDGFLDRIISIAGGENVLHDAPV
                     PFPMLSAEGVLKLNPEVIIDSIGGIAAQKIDRATILSGWQQVKNVAAVRNGRICLLEE
                     DVPLVPGPRMIELAERLAQCFHPESAAPRPAEESPDQP"
     gene            complement(1413..>1845)
                     /locus_tag="PKI05_13930"
     CDS             complement(1413..>1845)
                     /locus_tag="PKI05_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="polyvinylalcohol dehydrogenase"
                     /protein_id="HOA53349.1"
                     /translation="CALLHILRDGEKWTAEEVYFFDANHFANHHGGVVLVGDYLYGGD
                     GQNRGAPTCLDFLTGKIMWKEQPIDGGSAAVLYADGKLIFRYERGTVALIEANPKQFR
                     VISTFRPAVVDGPAWPHPVIHDGKLYLRAHNVLMCYDIKAQ"
BASE COUNT          431 a          561 c          505 g          348 t
ORIGIN      
        1 acctaccgct cgatggggac taccccgatg gcccatcgct cgatagggga atatcccacg
       61 aaagatggta cccaatttgc tcggttctga aagaaccccc aattgttcat cttggctgcg
      121 tcgagacagg gcacctagaa tctatccgaa aaagtccccc tctccctctg ggagagggtt
      181 ggggtgaggg cagacgaacg tcggaattga ttgagtaaac cggcagcgat tcaagccacc
      241 ctcacccccg agtcctctcc cgccgaacgg gcgaggggag attatcggat gggctccaac
      301 tactgcctgc tagggaaaac ccgagggcat cactcatccc gaagaaaact ttttcccggg
      361 aattttcctg gaccatccca cagtcgtccg caaaaagaag aaaaagtcac ggctgatcag
      421 gcgattcctc cgccgggcgc ggagccgcgg attcgggatg aaagcactgg gcgagtcgct
      481 cggcgagttc gatcatacgc ggcccgggaa cgagagggac atcttcttcc agtagacaga
      541 tccgcccatt tcggaccgct gccacattct tgacctgctg ccaaccgctg agaatcgttg
      601 cccgatcgat cttttgcgcg gcaatccctc cgatgctgtc gatgatgacc tcggggttca
      661 gcttgagcac tccttccgcg gaaagcatgg gaaacggcac cggcgcatca tgcaggacgt
      721 tctcgccccc ggcgatgcta atgatgcggt cgagaaaacc atcagggccg gccgcacaga
      781 tgtttcgcaa tcggccaggc tcaattgacc gctcgatcac gaaaaggaca cggggacgcg
      841 gcttgccttt ccagcgttcc tcaagatggg ccagtcgttg ccgccactcg cttgccaagg
      901 cctttgcccg atcttctact ccaaaaacgc gtcccaaaat ctccaacgaa tcgaacacgc
      961 catcgaccga gcggtgatca accgtgacgc ttttcaagcg gaatcgggca agtttctccg
     1021 agaagtcttg ctgcccggca ggaacgatca ccaggtcggg ctgaagtctg aggatcgcct
     1081 cccagttggg attaagatat ccgccgatct tcggaatggc tttcgcctcg gggggaaacg
     1141 aacaaaagtc cgtcaccccg accagccggt cgccgcagcc caaggcaaaa acggtctccg
     1201 taatgctcgg ggccatggag acgatccgtc ggggcgacat gtcgccgccg gcctggtggt
     1261 cggtttcagc cccgagccta tcatgaacag gcggggaact atcccagccg caacccgaaa
     1321 ccaaaatgac aaagaccagg aaagataaca ccgacctgca gacactcccc aagcgacaga
     1381 tagcggtttc ccgacaacgt tcgggggtgt cttcactggg ccttaatgtc atagcacatc
     1441 aaaacgttgt gggcacgtag gtagagcttt ccatcgtgga tgacgggatg gggccaggct
     1501 ggtccatcca ccacggccgg acggaaggta ctgataacgc ggaattgctt cggattggcc
     1561 tcaatcagtg ccaccgtgcc gcgttcataa cggaaaatca atttgccgtc ggcgtacagc
     1621 accgcggcgg accctccgtc gatcggctgc tccttccaca tgattttacc cgtcaaaaag
     1681 tccaaacacg taggagcacc acgattttgg ccatccccgc cgtaaagata atcacccaca
     1741 aggacgacgc cgccgtggtg gttcgcgaag tgattggcgt cgaagaaata cacctcttcc
     1801 gccgtccatt tttcgccatc ccgcagaatg tgaaggaggg cacac
//
LOCUS       DAOPTR010000862         1843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_49554_length_1843_cov_3.126957, whole genome shotgun sequence.
ACCESSION   DAOPTR010000862 DAOPTR010000000
VERSION     DAOPTR010000862.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49554_length_1843_cov_3.126957"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(539..>1843)
                     /gene="ptsP"
                     /locus_tag="PKI05_13935"
     CDS             complement(539..>1843)
                     /gene="ptsP"
                     /locus_tag="PKI05_13935"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOA53350.1"
                     /translation="RSLSHLTSPAILLARNLTPSEVANLDRKMIKGFVTELGGPTSHT
                     AIVAQGLGIPAVVGTGPFLAEVAGGDTVIVDGDRGIVIVHPDEETLREYDQEVRSIQA
                     FTAELEKLHDLPAVTLDGTPINIYGNIEFPHEAVLCLKHGAEGIGLYRTEFLYLGRSD
                     LPSEEDHYRAYREVIEAMGDRIVTIRTFDLGADKVAAQFGFEKEPNPCLGLRSIRLAL
                     RHLELFRTQARAILRASVLGNIQVMFPMIATVAELRQAKLIWADVMEDLAENGIPFRH
                     DIPIGIMVEVPSVAIMIDRFLDEVDFVSIGTNDLIQYTLAVDRTNRNVVSMYNPTDPA
                     VLRLIAYTIQEANRKGVPVSLCGQMASHPLYTMLLIGLGLRKLSVTSSAIPEVKKVCR
                     SVTLEQCERVARRALEMENARNIHAFLREQLVKVVPAFARMI"
BASE COUNT          444 a          529 c          478 g          392 t
ORIGIN      
        1 gatttttaac ccgtactgct tttccaggac ctggcgaatc acttcgtcca tcgtcgcgcc
       61 ccttcctcat cgaggaacgt acgactagcg cacctcgcaa gacggataga ctttctgcat
      121 ttgtctccac tgcgtacgcg acccatccgc ataggcggaa ccccagtccg atcagacaaa
      181 agaaatcgct ccacctcacg cgcaccccat ccctaggcac aatcagggcc tatctcctgc
      241 ccaaagagtc aagtcctaaa ccttttttta ggttagcaac accaccacac ggcttcaaca
      301 ttgttcgctt ccggcgagca tcatacacct tttgagcgac gagtggctgc acgcagagga
      361 actaacagtg cagggtttcg cacaacggct tccaaacatg atcagccgcg gaattcccac
      421 ggcttcgaga aaacagcatg ctgagagtag cgagtttttt gcccacaccg aaaccgaatt
      481 ggggccagac aagataaacc ttctccgggc atggcgttcc cgatgaaggc aagatcgctc
      541 aaatcatccg ggcaaaggcg ggaacgactt ttaccagttg ctctcgcaag aaggcgtgga
      601 tattgcgagc attctccatt tccaaagcgc gacgggccac gcgctcgcac tgctccaacg
      661 tcacgctgcg acagactttc ttgacctcgg ggatagccga cgatgtgacg cttagcttcc
      721 tcagcccaag gccaataagc agcatcgtat aaagaggatg acttgccatc tggccgcaca
      781 aacttacggg aacacccttc cggttggcct cctgaatggt ataggcgatg agccttagca
      841 ctgcggggtc ggtggggtta tacatggaga caacattgcg gttcgtccga tccaccgcca
      901 aggtgtattg gatgaggtcg ttggtgccga tactgacgaa atccacctca tcaagaaatc
      961 ggtcaatcat aattgccacc gaaggcacct ccaccatgat cccaatcgga atgtcatgcc
     1021 gaaacgggat accattttcg gccaaatcct ccatgacgtc ggcccaaata agcttggctt
     1081 gtcgtaattc ggcaacggtg gcaatcatgg gaaacatgac ctgaatgttt cccagaacac
     1141 ttgcccgcaa gatagcgcgg gcttgtgtgc gaaatagctc caggtgtcgc aaggccaggc
     1201 gaatactacg caatccaagg cagggattag gttccttttc aaacccgaac tgggcagcaa
     1261 ccttatcggc ccctagatca aacgtgcgga tcgtcacgat ccgatcgccc attgcctcga
     1321 taacttcgcg gtaagcgcga tagtggtcct cctcgctggg aaggtccgat cggccgagat
     1381 aaagaaactc cgtgcgataa agcccaatac cttctgcgcc atgcttcaag caaaggacgg
     1441 cctcatgggg gaattcgatg ttgccgtaga tattaatcgg tgttccatcg agcgtgactg
     1501 cggggaggtc gtgaagtttt tctagttcgg cggtaaaggc ctgaatgctg cggacttcct
     1561 gatcgtattc gcggagcgtt tcctcgtctg gatgaacgat gacgatgccg cgatcaccat
     1621 ccacaatgac tgtgtcacca ccggcgacct cggccaggaa tggcccggtc ccaacgaccg
     1681 ctgggatacc gagaccctga gccacgattg ctgtgtggct ggttgggcca cccaactcgg
     1741 taacgaaacc tttgatcatc ttccgatcga gattggccac ctcgctgggc gtgagattgc
     1801 gggccaagag gatggctggt gacgtcaaat gggacaatga ccg
//
LOCUS       DAOPTR010000863         1840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_49688_length_1840_cov_2.966387, whole genome shotgun sequence.
ACCESSION   DAOPTR010000863 DAOPTR010000000
VERSION     DAOPTR010000863.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49688_length_1840_cov_2.966387"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1170
                     /locus_tag="PKI05_13940"
     CDS             124..1170
                     /locus_tag="PKI05_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOA53351.1"
                     /translation="MAAIEIRGLSKTYRVTRKKEGLVATLRALFRPEYEYVHAVRSID
                     LTIESGEFVAFLGPNGAGKTTTLKLLSGIITPTSGEARVLGHIPWRRENAYRRRFALV
                     MGQKNQLWWDLPAAESFHLHQQIYGIPPDQFTRVRDELTEMLGVRQLLHRPVRELSLG
                     ERMKMELVAALLHSPQVLFLDEPTIGLDVVAQYTIQQFLREYQARTGTTVILTSHYMK
                     DVVVLCRRVVVIFDGQIIYDGSLTNLVDHLGDYKIITIRFSDGQVPTDLSRFGKIILR
                     EPPRVRLRVARNEATEVIRAVLARYIAEDVSVEDPPLEELIAALFASARNSRLSQERA
                     NSVVPEDTTHSTNH"
     gene            1217..>1840
                     /locus_tag="PKI05_13945"
     CDS             1217..>1840
                     /locus_tag="PKI05_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HOA53352.1"
                     /translation="MTYPSPAIIDTISAPLYRWRLWITILRISIQERIIYRGDFMLGT
                     LMRFLPIITQIFLWGAVFSALPTGNGSEGRIVGYSYHDMVAYYLLTMLSRAFSSMPGL
                     ASGITREIREGTIKRFIVQPIGLLEYLFFYRLAHKLVYYSVAIVPFAVVFYLCRGFFQ
                     ETPHGLVWATYIASLVLAFLLGFFLEATLGLLGFWFLEVSSFLFIYML"
BASE COUNT          373 a          498 c          505 g          464 t
ORIGIN      
        1 cacttctcaa gagacttggg gtgtgtaaag ccatcggcgg tgtcgtcttc caatgtgtgc
       61 tgtgactgat ggtccaaccg aaatgagttc cgacggtttt tcccgcgtcc tataatcgac
      121 agtatggctg ccatcgaaat taggggatta tcaaagacat accgtgttac ccgcaagaaa
      181 gaggggcttg tggccactct ccgggcgtta ttccgtcccg agtatgaata cgtgcacgcg
      241 gttcggtcaa ttgatctcac gatcgaaagc ggcgagttcg ttgcgttcct cgggccaaat
      301 ggggcgggaa agacaacaac cctcaagctt ctttcgggaa tcattacgcc cacctcgggc
      361 gaggcccgcg tccttggcca catcccctgg cgacgggaga acgcttatcg acggcgcttt
      421 gccctggtga tgggacagaa gaaccaattg tggtgggacc tgccagcggc tgaatcgttc
      481 caccttcacc aacagattta cggcatcccg ccagaccagt tcacccgagt gcgcgatgaa
      541 ctaacggaga tgctaggagt tcgccagctc ctccaccgtc ctgtgcggga gttatccctg
      601 ggggagcgca tgaagatgga attggtggcc gcactgttgc actcgcccca agtgctcttt
      661 ctcgacgagc cgacgatcgg cctggacgtg gtggcccagt acacgatcca gcagttcttg
      721 cgggaatatc aggcccggac cgggacgacc gtcattctga caagccacta catgaaggac
      781 gtggtcgtac tgtgtcggcg agttgttgtc atttttgatg gacaaattat ttatgatggt
      841 tccctaacca accttgtgga tcacctgggg gattacaaaa tcatcacaat acggttttcc
      901 gatggacagg ttcccaccga tttgtcccga tttggaaaaa tcatccttcg ggaaccaccg
      961 cgggttaggc tgcgggttgc gcgcaacgag gcgacagaag tcattcgggc tgtccttgcc
     1021 cgatatatcg cggaggatgt tagcgtggaa gacccgcccc ttgaggaact gatcgccgcc
     1081 ctgtttgcct ccgcgagaaa ttctcggtta tcccaggaac gggcaaattc ggtagtaccg
     1141 gaagacacga cccactcgac aaatcactag cataagattg atagcacgct ctcctggtcc
     1201 tgaacctaag cgacttatga cgtaccccag ccccgctatc atcgatacca tttccgcacc
     1261 gctttaccgg tggcgcttat ggatcacgat tctgcggatc agtatccagg agcgaattat
     1321 ttaccgaggc gatttcatgc tggggacgct catgcggttt ctccccatca tcacacagat
     1381 cttcctctgg ggtgccgtct tctcagcctt gcccactggt aacggtagtg aaggacggat
     1441 tgtgggctac agttaccacg acatggtggc ctactacttg ttgacgatgc tcagtcgggc
     1501 gttttcgagc atgccgggct tggcgtcggg cattacccgc gaaattcggg aaggaacgat
     1561 caagcggttc atcgtccagc cgattgggct gctcgaatac cttttcttct atcgcctggc
     1621 tcacaagctc gtgtactaca gcgtggcgat cgtcccgttt gcggtggtct tttatctgtg
     1681 tcggggtttc tttcaggaaa caccgcatgg gcttgtttgg gcgacgtata ttgcgtcgct
     1741 tgttttggca tttctgcttg gtttcttctt ggaagcgacg ctcggacttc ttggcttctg
     1801 gttcctcgag gtcagttctt tcttgttcat ctatatgctt
//
LOCUS       DAOPTR010000864         1837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_49803_length_1837_cov_3.228395, whole genome shotgun sequence.
ACCESSION   DAOPTR010000864 DAOPTR010000000
VERSION     DAOPTR010000864.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49803_length_1837_cov_3.228395"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1837
                     /locus_tag="PKI05_13950"
     CDS             <1..>1837
                     /locus_tag="PKI05_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="organic solvent tolerance protein OstA"
                     /protein_id="HOA53353.1"
                     /translation="AGMILNAELLTRIPDYEGYLRLRADILQQMNETQFFAQNLFVTS
                     SRMGVPGYRLQTQFAYVEDRIQPVLDPTTRQVVIDPQTGQPAVIHRPWALARDNFIYL
                     RNIPIFYWPVLAGDLTKPTYYVNRIRLRHDSIFGTQVLTNWDAFQLFGIRNRPEGMDW
                     SLSLDYLSERGLGHGTSLTYSRDGFLGLPGYATGLADFWGIYDNGLDNLGRGRRALAP
                     EADYRYRLLWQHRQYLSEEYQLTGEVGLISDRNFLEQYYEREWDELKDQTTSLSLKRI
                     YGNASWDLSASVRLNPFFTQTDWLPRFDHYKLGESFFNDAFTWYAHTSIGLARFRTAS
                     ATSNPEDLAAFSYLPWETSSLTGDPLSTFSERMITRQEIDWPFQLGVIKIVPYALGEL
                     GHWGEDRSGDSLQRGYVQLGARANLPLWAVNPYIESELFNVHGIAHKINFKAEAFWAS
                     SNRDLDALPLYEPLDDDAIEAFRRRLGVLTFGPTNGFGGPPAPYAFDERSYAVRSGLA
                     SWVTSPSMEIADDLTLIRLGVEQRWQTKRGMPGSRRIVDWITFDTHFSLFPDSSRDDF
                     GRFVGLLDYDARWFVGDRLTLASTGLFDFFDSGQKLVTFGGMLSRP"
BASE COUNT          364 a          552 c          498 g          423 t
ORIGIN      
        1 gagcagggat gatcctcaat gccgaattgc tcacacgcat tcccgattat gagggttacc
       61 tccgtcttcg ggccgacatc ctccaacaga tgaacgagac gcaattcttc gcccaaaatc
      121 tcttcgtcac atcaagccgc atgggcgttc ccggttatcg attgcaaacg cagttcgcct
      181 acgtggagga ccggattcag ccggtgcttg accccacgac acgccaggtg gtgattgacc
      241 cgcaaaccgg ccaaccggcg gtgattcacc gtccctgggc cctggcccga gacaacttca
      301 tctacttgcg caatattccc atcttctact ggcctgtcct ggccggggac ttgaccaagc
      361 cgacgtacta tgtgaaccgc attcgccttc ggcacgacag catctttggc acgcaggtcc
      421 tgaccaactg ggacgccttt caactttttg gcattcgcaa tcgaccggaa ggaatggatt
      481 ggagcctgag cctagattac ctcagtgagc gtggcctggg acacggaaca tcgctgacct
      541 attcgcgtga cggatttttg ggattacccg gttacgcgac cggcttggcc gacttctggg
      601 ggatttatga taacggtctg gacaacctgg ggcggggacg acgggccttg gccccagaag
      661 ccgactaccg ctaccgccta ctttggcaac accgacagta tctttccgag gaatatcagt
      721 tgaccggcga ggtggggctg atctcggacc gcaatttcct ggagcaatac tacgagcgag
      781 aatgggacga acttaaagac cagacaacca gtctctcgct caaacggatt tacgggaacg
      841 cctcgtggga cctctcggct tctgttcgac tcaatccgtt cttcacccaa acggattggc
      901 tgccgcggtt cgatcattac aaactgggcg agtccttctt caatgacgcc ttcacctggt
      961 atgcccacac cagcattggc ctggcacgtt ttcggacggc ctccgccact agtaatcctg
     1021 aagatctggc cgccttttcc tatcttccgt gggagacgtc gtctctaacc ggtgatcccc
     1081 tcagcacgtt cagcgaacgc atgattacgc ggcaagagat cgactggccc tttcaactcg
     1141 gcgtcatcaa gattgtcccc tatgcactgg gcgaactggg gcattggggc gaagaccggt
     1201 ctggggattc cctccagcgc ggttacgtcc agcttggtgc ccgcgccaat ctcccccttt
     1261 gggcggtcaa tccctacatt gagagcgagc tattcaacgt tcatgggatt gctcacaaaa
     1321 taaacttcaa ggctgaggcc ttttgggcca gttccaaccg cgacctcgac gccctgccgc
     1381 tttacgagcc gctcgatgac gatgccatcg aggccttccg gcggcgactg ggggtgttga
     1441 cctttgggcc gacaaatggc tttggtggcc caccagcccc ttacgccttt gatgagcgat
     1501 cctacgcggt acgcagcggg ctggcctctt gggtgacatc acccagtatg gaaattgctg
     1561 atgacttgac gctcattcgc ttgggggtgg aacagcgatg gcaaaccaag cgtggcatgc
     1621 ccggttcacg acgcatcgtc gattggatca cgtttgatac gcatttctcg ctatttcccg
     1681 attcctcccg ggatgacttt ggccgattcg tcggacttct cgactacgac gcccgctggt
     1741 tcgtcggcga ccgattgacg ctggcctcga cgggtctgtt cgactttttc gattcgggtc
     1801 agaaacttgt aacatttggc gggatgctca gtcgccc
//
LOCUS       DAOPTR010000865         1830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_50083_length_1830_cov_3.216901, whole genome shotgun sequence.
ACCESSION   DAOPTR010000865 DAOPTR010000000
VERSION     DAOPTR010000865.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50083_length_1830_cov_3.216901"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1830)
                     /locus_tag="PKI05_13955"
     CDS             complement(<1..>1830)
                     /locus_tag="PKI05_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53354.1"
                     /translation="AQVIIGEGLQIRGLRVLDPHGDRHSGLLLDVDELFLAGPVDYQQ
                     LLAKRLQIKRVVIRRPHLHLVNRQQDGWNIARLFPLPRGSGQCVPLEIENGLIEIVDM
                     RKSPASGLTLRDVQLSLTPPGPTTEDSAPLWNLRGTMTGDFLRRIDIEGSIDPGSLRT
                     RLAGRVDSIEFGPEFQSALPLEITRRLESLQHLRAQLALTFQLYIDMQSPPRITFDVA
                     GQIVRGRIDDPRLPHPISDIRASFACRNSGIEVRNLSATMGQTVFNVSSFTLFGWTLS
                     DPFDLEARCEHLEFDPAIAQLLPTVLQEEWPKYFPQGLVDLSIQFNSRREGSQFRVVA
                     DLQNVSFAYYKFPYRLQQTFGRMELTPERLTIQLVARANGQPVEIRGQVLTPLKSPRS
                     QIFVSGKEIALDDRLVDAILNPKTRRTIRSLQLAGTADFWLSLWQDKPGEELHRHLEA
                     TLNRCSIKYQGFPYPISNITGRLIMRDEAWSFEELKGSQGSAQITADGTLLSDASGSV
                     LTLRFRGRQIRLDNTLRDAFLLEHVRSAWNDLQPNGLIDVEGVLSWHTGEPMKLSFSA
                     RPSDRSVTLEPTGFPYSMERVDGTFIYENGTILVNDFQAFHGNT"
BASE COUNT          456 a          477 c          493 g          404 t
ORIGIN      
        1 tgtgttgccg tgaaaggctt gaaaatcgtt gacgaggatt gttccgtttt cgtagatgaa
       61 cgttccatcc acgcgttcca tgctataagg aaacccggtt ggttctagcg tcacgcttcg
      121 gtcgcttggc cgtgcagaga agctcagctt catcggttcg ccagtatgcc aactgagtac
      181 tccctccacg tcgatgagac cattgggctg gagatcattc caagcacttc gcacgtgctc
      241 taataagaaa gcgtcacgaa gggtgttatc caatcggatt tgccgacctc gaaatcgtag
      301 tgtcaaaacg gaaccactgg catcagaaag caacgtcccg tctgcggtga tttgggccga
      361 accttggctt ccctttaact cctcaaagga ccaggcttca tcccgcataa tgagtcggcc
      421 cgtgatgttg gagatgggat aaggaaatcc ctgatatttg atggagcagc gattcagggt
      481 tgcctcgagg tgacgatgaa gttcctcgcc tggtttatcc tgccaaaggc tcaaccaaaa
      541 gtccgcggtt cccgccaatt gcagggagcg gatagtcctg cgcgtcttgg gatttaaaat
      601 ggcatcaacc aaacgatcat ccagggcgat ctcttttccg ctgacgaaga tttgcgacct
      661 tggggatttt aacggcgtta acacctggcc acgaatctcg acaggttggc cattggcccg
      721 cgcaaccaac tggattgtca atcgctcggg agtcaattcc atgcggccaa aggtttgctg
      781 caaacggtaa ggaaacttgt aataggcaaa tgaaacattt tgcagatcgg ccaccacacg
      841 gaactgcgag ccttctcttc ttgaattgaa ttgaatcgat aggtcaacta gcccctgggg
      901 aaagtatttg ggccattcct cttggagtac ggtaggaaga agttgagcaa tggcgggatc
      961 aaactcgaga tgttcacacc gggcctccaa atcaaagggg tccgataatg tccagccgaa
     1021 cagagtaaaa gagctgacat tgaacaccgt ttgtcccatg gtcgccgaca gattacggac
     1081 ctcgatcccc gaattgcgac aagcgaagct tgcccgaata tccgaaatgg ggtgtggcag
     1141 tcgaggatcg tcgattcgcc ctcgcacgat ctgtcccgca acatcgaaag taatccgcgg
     1201 gggactctgc atgtctatgt aaagctgaaa cgtcaaagcc aattgtgccc gcaagtgttg
     1261 gaggctttcc aaacgtcgcg tgatctccag gggaagcgca ctctggaatt ccgggccgaa
     1321 ttcgatggaa tccacccggc cggcaagacg agttcgcaaa ctgccaggat cgatgctgcc
     1381 ctcgatgtca atacgtctca aaaaatcgcc ggtcatcgtg cccctcaggt tccagagggg
     1441 agcgctatct tcggtcgttg ggccaggagg agttaacgac aattggacat cccgcaaagt
     1501 caatccggag gcgggactct tccgcatatc cacgatctcg atcagcccat tttcgatttc
     1561 gagcggaacg cattggccgg aaccacgcgg cagcggaaaa agacgtgcaa tattccagcc
     1621 atcttgctgc cgattcacga gatggaggtg gggccgacga atgacaacgc gcttaatctg
     1681 gagcctcttg gccaagagtt gctggtagtc cactggaccg gccaggaata gctcatcaac
     1741 atcaagaagg agacccgaat ggcgatcacc atgaggatcg aggacacgta aaccgcgaat
     1801 ttgaagtcct tcgccgataa tgacttgcgc
//
LOCUS       DAOPTR010000866         1826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_50220_length_1826_cov_3.178430, whole genome shotgun sequence.
ACCESSION   DAOPTR010000866 DAOPTR010000000
VERSION     DAOPTR010000866.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50220_length_1826_cov_3.178430"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..610)
                     /locus_tag="PKI05_13960"
     CDS             complement(104..610)
                     /locus_tag="PKI05_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53355.1"
                     /translation="MRPINDGARRNMLCHNTRDELFRLLDALLNETISEEQFRRLEAI
                     LLAEAEARQIFRRYLTLHHQLRTLAKSWAEEKLVKLAEGPNPEVPPVSPSGQMGQGIL
                     YGFLIAIGLALLAGALWFRHIRAETPCPPTTFSESISHAPLVCDSPGSCRLSDKMASR
                     PSEAQPTK"
     gene            complement(696..1268)
                     /locus_tag="PKI05_13965"
     CDS             complement(696..1268)
                     /locus_tag="PKI05_13965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOA53356.1"
                     /translation="MGYDSYALETRDWVELAAAGTDELTKEFLEQFTRHHERIFGYIF
                     GLVHNEQDARDIFQKVSLLLWQKFETFDQEADFFAWACGVAYNEVRNYFRTSSRNRLC
                     FNQELLDTLATERDDEDEESDARLEVLRDCLAQLPQHERELLMEVYAEGRSIREIAER
                     RGKAAQTLYNRLNLIRRKLFIEVQQALAQN"
BASE COUNT          390 a          484 c          514 g          438 t
ORIGIN      
        1 cagttgaagc ggcttcgctc aagcgaagct gatgtgcgct acgttacttg acaagcgtgt
       61 cccagcaatt ggccggagaa gggattcctc tgtatgggaa cacttatttg gtaggctgtg
      121 cctcggaagg tcttgacgcc atcttgtctg agagccggca cgagcctggc gagtcgcaaa
      181 ccagtggggc atgcgaaatg gattcggaga aggttgtagg ggggcacggc gtttcggcac
      241 gaatgtgtcg aaaccatagg gctccggcca gtagggccag accaatggct atgagaaagc
      301 cgtaaagaat cccctggccc atctgaccag agggtgagac cggtgggact tcggggttgg
      361 ggccttcggc caacttgacg agtttttcct cggcccagct ttttgccaat gtgcggagct
      421 gatgatggag ggtcaagtat cggcggaaga tttggcgggc ttccgcttcg gctaagagaa
      481 tggcttcgag acgccgaaac tgctcctcgg agatcgtttc gttgagcagc gcgtcaagca
      541 accggaagag ttcatcgcgc gtattatggc acaacatgtt ccgtctcgca ccatcattaa
      601 tcggccgcat gacgcccgct ccttgcagcg cgctacgaca tcaccgggat gccgtagcga
      661 gggctggact acatcactgc atgggagctt ggccgtcagt tctgggccag ggcctgttgg
      721 acttcaatga acagttttcg gcggatgagg ttgaggcgat tatacagcgt ctgggctgcc
      781 tttccgcgtc gttccgcgat ttcgcggatg gagcgacctt ccgcatagac ttccatcagc
      841 aactcccgct cgtgctgagg aagctgcgcc aggcaatccc gaaggacctc caggcgcgcg
      901 tcgctctctt cgtcctcgtc atcccgttct gttgcgagcg tatccaaaag ctcttgatta
      961 aaacagagac ggttgcgtga ggacgtgcgg aaataattcc tcacctcatt gtaggccaca
     1021 ccacaggccc atgcgaagaa atccgcttcc tgatcgaacg tctcgaactt ttgccacagg
     1081 agcaggctga ccttttgaaa aatgtcccgc gcatcctgct cgttgtggac aagaccgaag
     1141 atatatccaa aaatccgctc gtgatgtcgg gtgaattgct ctagaaactc cttcgtaagt
     1201 tcatcggtcc ctgccgcagc aagttccacc caatcccttg tctccagggc atagctgtcg
     1261 tatcccatcg cacggctcct cgttcattgt tccaacgctg gcggctcgca cagcgattgc
     1321 cggcttccac gcgacaaatg ccgtccctgg ctgagaaatc aggggattgt tttcacccga
     1381 gttggtcttg tgtttcgcct gatttggggg gtgaaggatc ttctatgaag ctcaatcagg
     1441 actgacaaat atcgttatga cgttccgtcc tttgatagca ctggttcagt ctcctacccc
     1501 aggtgcacgt acaggtggga tttgatttcg aggcaggcaa cctttcttgg tccaaagatt
     1561 actcgtacat cctaggctta ttttaccgac taaaacggaa aagacagtgc cctttgcgag
     1621 aaaatagcgg aaaaacaccg ctgcagtggg gattagatta ctaattggca tgggatttga
     1681 acgcaccgcg taccggatat cccccaagtg atggggtctt ttggcctgcc gctgctcgta
     1741 ttagtcgcag tgtactgatg atcactacac gcccggtgtg acgaatttgc tttgttgtaa
     1801 cacgctagaa gctggccgtg tctcgt
//
LOCUS       DAOPTR010000867         1823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_50317_length_1823_cov_3.758484, whole genome shotgun sequence.
ACCESSION   DAOPTR010000867 DAOPTR010000000
VERSION     DAOPTR010000867.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50317_length_1823_cov_3.758484"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            772..1347
                     /locus_tag="PKI05_13970"
     CDS             772..1347
                     /locus_tag="PKI05_13970"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOA53357.1"
                     /translation="MSLLKTLAGIVVVAVNLVSQKLAPREQTPVELRVGDVAPDFELP
                     GSDGKTYRLADFRGQKIVVLAWFPKAFTPGCTRECRVFAERADEFANLPVAFFTASVD
                     TPSENKKFAESVGARYPILSDPDRKVALAYGVVSSPRDFARRWTFIIGLDGKILHIDK
                     NVKVDTHASDVARWIKEYLKANPPAGDSGKR"
     gene            complement(1535..>1823)
                     /locus_tag="PKI05_13975"
     CDS             complement(1535..>1823)
                     /locus_tag="PKI05_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53358.1"
                     /translation="VAIGKNPVDVIKRAIDDAVSQGDQMAPAFELCLKVDDLVATAAK
                     LNPQDSRLQTLKANLEQCEGVQLLRKKVDFTSQQMVVDFEMDLNWLRLLKR"
BASE COUNT          410 a          503 c          476 g          434 t
ORIGIN      
        1 aagatgtgca atattcgctg ccgcttcatt gccctccaca gtcacgaagc gttgctgtgt
       61 cacggctaag tctccttcaa aaaaacgcct gtcacatatt cgtaatcatc ggcagccgca
      121 ctcattcact cagcccaagg tcaggacgaa cgcggtcaac cgacgtgtca aacttcactc
      181 cgtaattctc gtacccaaca gtgacaaaag tccgcttaca agaattgata tacgcaccca
      241 ccgactagga tggttcgccg tacccaaagc cgcagaccca ctcgtcccgg ctatattaac
      301 ccgatagcct aacgcttttc caggccattc gcaagtcgtg cagtaacaga atgattcgta
      361 tttggagcct atcccaaaac tccctctccc tctgggagag ggttggggtg agggcaagcg
      421 accgttggag tcgatcgaaa aaaccggtag cgatcggagc cacccttacc gccagcccct
      481 ctcccgccga acgggcgagg agaggttggg ggataggctc ttagcagtta ctggtgaaca
      541 gtgtcgccag agattcattc cggccgacaa cagttctttg gaaccgttgc gagatgctct
      601 ttttggtttt ctcccctcat ggaacgggcg tttattctct ctgacagatc ttggcagatt
      661 ctagcggtgg gtagactttc ggcgacatcg ccataaaatg acatcggtag ttgtcgtaaa
      721 taagtctcaa ccagttggtg ccgtatccaa acgacgattc gaggtgaagc catgtcgcta
      781 ttgaagacac ttgcaggcat cgttgttgtc gctgtgaatc tcgtttccca aaaacttgcc
      841 ccgcgggaac aaacgcctgt cgaactccgc gttggtgatg tcgccccgga ttttgaactg
      901 ccgggcagtg acgggaaaac ctaccgtctg gccgactttc gtggccagaa gatcgtggtc
      961 ctggcctggt ttccgaaagc ctttacgccg ggatgtaccc gtgagtgccg cgtttttgcc
     1021 gagcgggccg acgagttcgc caatttacct gtcgcatttt ttacagccag tgtcgataca
     1081 ccttcggaaa acaaaaaatt tgccgagtct gtcggtgctc gttatcccat tttgagtgac
     1141 ccagaccgaa aggtcgcgct ggcctatggg gttgttagtt caccgcggga cttcgcgcgt
     1201 cgttggacat ttattatcgg cctcgatggg aagattcttc acattgataa gaacgtcaag
     1261 gtcgataccc acgccagcga tgttgcccgg tggataaaag aatacttgaa ggccaatccc
     1321 ccggcagggg attcaggcaa acggtgaatt tcacaccgga actctttctg ggcgatccac
     1381 ctctgggtgc gtgttggcga gatgagcacg ggcccatcat aagcagactc cccaggttgg
     1441 acgcccgggg agtctgatga acagcaaacc acacggattg tgcggattgc ctccaatatg
     1501 gctgcttgct acccgtctgc caattctgtc acctttaccg cttcaataag cggagccagt
     1561 tcaggtccat ctcaaaatcg acgaccatct gctggcttgt gaaatcgacc ttcttccgca
     1621 agagctgaac accttcgcac tgctcaaggt tagccttgag tgtttggagc cggctatctt
     1681 gcggattcag tttagcggcg gtggcgacca agtcgtcaac cttgagacat agctcgaagg
     1741 ccggggccat ttgatcgccc tgagacacgg cgtcatcaat ggcccgtttg atgacatcaa
     1801 cggggttttt gccgatggcc acg
//
LOCUS       DAOPTR010000868         1823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_50321_length_1823_cov_3.591629, whole genome shotgun sequence.
ACCESSION   DAOPTR010000868 DAOPTR010000000
VERSION     DAOPTR010000868.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50321_length_1823_cov_3.591629"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PKI05_13980"
     CDS             complement(<1..683)
                     /locus_tag="PKI05_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53359.1"
                     /translation="MSNEANTQSSSGDAPTIEFAPSIGVADSQLPSEESPALRCGPVK
                     FVTGSGQDWTCEIDAVLRRRLRLALWVLGLGFAVFLPVHYYFGEIATRTGGFLFLGHG
                     SLTLVLVGCAVWLTLARQSPSFTTLRLVEVAAFGLTGVFFGFLSWNSMEKQCLEIGQV
                     RFEGGFWLILIYTYALFIPNSFWRSAIVIGSIALVPIVLLYVGWLFHPELARAMTTEH
                     TVVIPLVFVV"
     gene            complement(1151..>1823)
                     /gene="bioB"
                     /locus_tag="PKI05_13985"
     CDS             complement(1151..>1823)
                     /gene="bioB"
                     /locus_tag="PKI05_13985"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HOA53360.1"
                     /translation="TYCIAISGRSPTPSELDRLCAVIRTIKETLPLRICLSPGFLSRQ
                     QAEQLKEAGLDRINHNLNTSQSFYPKICSTHTYEERLGTLRAAREVGLELCCGGIIGM
                     GETVDDVVDFALTLAELRPEAIPINFLIPIPGTPVQDRHELTPLYCLKVLALVRFASP
                     QSDLRIAAGREVHLGPLQSLGLFVANSIFVGDYLTTRGQPPEEDFTMIRALGFEIEQV
                     CHAQG"
BASE COUNT          451 a          522 c          499 g          351 t
ORIGIN      
        1 actacgaaaa cgagcgggat gacaacggtg tgctccgtcg tcatggcccg ggccaattcg
       61 ggatgaaaga gccagcccac gtacagcaag acaatcggca caagggcaat cgacccgatg
      121 acaatggccg atcgccagaa agagttcgga atgaacaacg cgtacgtgta gatgagaata
      181 agccaaaacc caccttcaaa cctcacctgc ccaatttcga ggcattgttt ttccatggag
      241 ttccatgata ggaacccaaa aaagacgccc gttaggccaa aggcagccac ttccacaaga
      301 cgtagtgtcg tgaaactcgg cgactgtcgt gctagtgtca accacacggc acaacctacc
      361 aggacgagcg tgagcgagcc atggcctaga aagaggaacc cgcccgtgcg ggtagcgatc
      421 tcaccgaagt aataatggac cggtaagaag acagcaaaac ccaagcctaa aacccaaagg
      481 gcaagtctca gtcgcctccg caggaccgcg tcgatctcac aggtccaatc ctgaccagag
      541 ccggtcacaa acttaaccgg gccacagcgg agcgctggtg actcttcaga tggaagttgg
      601 ctgtcagcca ccccaatcga aggagcaaat tcgatagtgg gggcatcgcc cgatgacgat
      661 tgcgtattgg cctcgttcga cacgttatgg attccctact gacggggcaa acttggaagt
      721 tcatcgtgcc ccaaaacgcc cgcacggcaa aacccgtgtc cgagcagctc aaatcaccat
      781 attatacggt ggcctggcgg cgggacatct taaagctgct tcgccagcgt ctgaccccgc
      841 gaggcaaggc gtcctttcac tatggccccg gttggccgga tacgccccac ctgtttttcc
      901 cagtctttct atccttccta cccccagtac gtgaagccgc ggaacgttcc aagagtctat
      961 ctcacaacct cccctcgccc gttgggcggg agagggagca gggctgaacg tagctcgaat
     1021 cggcggcgtt ttctcggtcg actccaacga tcgcttgccc tcaccctaac cctctcccag
     1081 agggagaggg aatttgggga taggctctga gctcattctt gcaagagaga cctaaaaaca
     1141 ctttagccgt ttacccctgg gcgtgacaca cctgctcaat ttcgaacccc agggcgcgaa
     1201 tcatggtgaa atcctcttcc gggggttgac ctcgcgtcgt caagtagtcg cccacgaaaa
     1261 tggaattggc cacgaataat cccaacgact gaagtggccc caggtggact tcacggcccg
     1321 cagcgatgcg aagatctgat tgagggctag caaatcgcac aagcgcaagg accttaaggc
     1381 aataaagggg cgttagctcg tgccgatctt gaactggtgt tcccggaatg gggatgagga
     1441 aattgatcgg gatggcctcg gggcggagct cggccaacgt caaagcgaaa tccacgacat
     1501 cgtcaaccgt ttctcccatg ccgataatgc caccacagca cagttccagt cccacttccc
     1561 gcgccgcacg taacgtgcca agccgctcct cgtacgtatg ggttgagcaa atctttgggt
     1621 agaacgattg gctggtattg aggttgtggt taattcggtc cagccctgcc tccttgagtt
     1681 gctctgcttg ttgtcggctg agaaagcccg gagacaagca aatccgcagg ggcaacgtct
     1741 ctttgatagt gcggatgacg gcacacagtc ggtcaagttc tgaaggtgtc ggggagcgac
     1801 cggagatggc aatacagtag gtg
//
LOCUS       DAOPTR010000869         1823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_50325_length_1823_cov_3.389706, whole genome shotgun sequence.
ACCESSION   DAOPTR010000869 DAOPTR010000000
VERSION     DAOPTR010000869.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50325_length_1823_cov_3.389706"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1823)
                     /locus_tag="PKI05_13990"
     CDS             complement(<1..>1823)
                     /locus_tag="PKI05_13990"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOA53361.1"
                     /translation="WETFLQLHPLDERAPKILYLFGEMNARQKLWDAAINDWRRLAEK
                     YPDNPWGQQARFAIAQLYETQLHDAEKALEEYRKVGNPLSEAAAAAIARLTAKTLNVE
                     TPRVFRSDETPKVRLITRNIEKVSVAVYRLDPEAYFRKMHTLTGVEQLDVALIDPDTT
                     FEFPIPDYRPYVELETFVPIPLPGGRKCGAAAVTISSATFEATTLLLQSNLDVIVKCS
                     RDEVLVFSQNMITGQPWPGVRVLLAGPDGILHEAITGKDGVFQAALPALRQLENVQVL
                     AIANDHLASNALSLAGLQPARGLTDRGFIYTDRPVYRAGQIVNVRGIVRFAQAKNEAA
                     STDSPLSADKLVVPQDQAFTLRIVDPQDRILRQVDVTLDRFGCFHDQFLLPDEAEPGH
                     YRIVLTDVANHTFQGGFRVEVFQLEPIQITVDVPRRVFYRGEKIEGTIRVAYVFGTPL
                     AEKLIRYQLAEQPVQTATTDAQGEIHFSFETRGYLETAMLPLVVSLPERNLAVTETFV
                     LTTHGFTVDVTTPRRVYLPGETFEVSVTTRDASGQPIAQKLTLKVLKRTRVGRVLGEV
                     LMEEQAVETDVEGRAKPVLHIDQGGEYILRIEGTDRFEHVVS"
BASE COUNT          394 a          519 c          530 g          380 t
ORIGIN      
        1 gaaaccacat gctcaaaacg gtccgtgcct tcgattcgga ggatgtattc accaccctga
       61 tcgatgtgca acacgggctt ggcacgccct tccacatcgg tctccacggc ttgctcttcc
      121 atcaacacct cgcccaacac gcggccgacc cgcgttcgtt tgaggacctt gagtgtcaac
      181 ttttgggcga tgggctgccc cgaggcatct cgcgtggtca cgctcacttc aaaggtttca
      241 cccggtagat agacccgccg cggagtcgtc acatcaacgg taaatccgtg tgtggtaagg
      301 acaaatgttt ccgtgaccgc gaggttccgc tcgggcaagg agaccaccag cggcagcatc
      361 gccgtttcca gatatccccg cgtctcaaag ctaaagtgaa tttcaccttg ggcatcggtc
      421 gtggccgttt gaaccggctg ctcggccaac tggtagcgta tgagtttctc ggctagcggc
      481 gtgccaaaga cgtaggccac gcggatggtc ccctcgatct tttccccgcg atagaaaaca
      541 cggcgtggga catccacggt gatttggatg ggttcaagct gaaagacctc cacacggaag
      601 ccgccctgga acgtgtgatt ggcaacgtcc gtcaaaacaa tccggtagtg ccccggctcg
      661 gcttcgtcgg gcagcaaaaa ctggtcgtga aagcagccga aacggtcaag cgtcacatcg
      721 acttggcgaa ggatgcggtc ttgcggatcg acaatccgca gggtgaaagc ctggtcctgc
      781 ggaacgacca gtttatccgc agaaagtggt gaatcggtgc ttgcggcttc gtttttcgct
      841 tgggcaaaac ggacaatgcc tcggacattg acaatctgcc ccgcgcgata gacggggcga
      901 tccgtgtaga taaagccccg gtccgttaag ccccgagcgg gttgcaagcc cgcgagggac
      961 aaggcattgg aagcgagatg atcgttggcg attgccaaaa cctgcacgtt ctccaactgc
     1021 cgcaaggcgg gaagggccgc ctggaaaacc ccatctttgc cggtgatggc ttcgtgcaaa
     1081 ataccgtcgg gaccggctag gagaacccgc acacccggcc aaggttggcc ggtgatcata
     1141 ttttgactaa atacgagcac ttcgtcccgc gaacacttga caatcacatc caggttactc
     1201 tgcaagagga gcgtcgtggc ctcgaacgtc gcgctgctaa tggtgacggc tgcagcgccg
     1261 catttacgtc cgccgggcaa tgggatgggg acaaaggtct caagctccac atacggccga
     1321 taatcgggga tgggaaattc aaacgttgtg tcgggatcaa tcaaagccac atcgagctgc
     1381 tccacacccg tgagtgtgtg cattttgcgg aagtaagcct ccgggtcgag tcgatacacg
     1441 gcgaccgaaa ccttctcgat attgcgggtg atgaggcgga ccttaggtgt ctcgtcggag
     1501 cggaagacgc gaggcgtttc cacattcagg gtctttgccg tcagccgggc aatcgccgcc
     1561 gctgccgctt cggacagagg gtttcccacc ttgcggtact cttctaaggc cttttccgca
     1621 tcatgcaatt gggtttcgta gagttgggca atagcgaagc gggcctgctg cccccaggga
     1681 ttgtcaggat atttctcggc caaccgtcgc caatcgttga tggcggcatc ccagagtttc
     1741 tgtcgggcgt tcatttctcc aaacagatac agaatcttcg gagcccgctc atcaagggga
     1801 tggagttgca aaaaggtctc cca
//
LOCUS       DAOPTR010000870         1813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_50706_length_1813_cov_2.246871, whole genome shotgun sequence.
ACCESSION   DAOPTR010000870 DAOPTR010000000
VERSION     DAOPTR010000870.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50706_length_1813_cov_2.246871"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..773)
                     /locus_tag="PKI05_13995"
     CDS             complement(174..773)
                     /locus_tag="PKI05_13995"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOA53362.1"
                     /translation="MKPTDILRAEHRVIEQVLAVLEKMATQAEASKSVDVESARQVID
                     FIRHFADRCHHAKEEDRLFPALEARGFPRQGGPTGVMLAEHEMGRECVRDMDRALGEL
                     DHAPEAALSTFVTAARRYVALLREHIGKEDNVLFVLAERTIEEDAQAQLLSEFEAVEK
                     EHVGEHVHEKYVQLAESLMERYGIDKQSIPRSGDFFCCH"
     gene            complement(1024..1449)
                     /locus_tag="PKI05_14000"
     CDS             complement(1024..1449)
                     /locus_tag="PKI05_14000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOA53363.1"
                     /translation="MISRTAIHGLRALMILAEMPPDQYAGTAELAKQIKAPRNYLGKL
                     LQRLADEGLLESQRGKGGGFRLARPPERITIYDVVEPLDRVSRWEGCLLGRSRCSDEA
                     PCPVHVRWAKVRAVYLEFLRTTTLDELVAQRRESPSCRS"
BASE COUNT          392 a          543 c          447 g          431 t
ORIGIN      
        1 ggtcgttccc ttgtgtgcca tgctggtgtt gaccgttacg cctgacacct accgcgggca
       61 tggggaaact cttcatccgc cacccgctgt tcaatttgcg ataatgtctt ttcacaaatc
      121 actcgctgat gtgcgattca aatatcccct ttgcgactta tgcatgggtt cgcctagtgg
      181 caacaaaaga agtcgccgga acgggggatg ctctgcttgt caattccgta tcgctccatc
      241 aggctctcgg cgagctgaac atatttttcg tgaacgtgct cacccacgtg ctctttttca
      301 actgcctcaa actccgaaag gagctgtgcc tgggcgtctt cctcgatggt gcgctcggcg
      361 aggacgaaga ggacgttatc ctcctttcca atgtgctcgc gcaagagagc gacgtaacgc
      421 cgtgccgccg taacaaacgt acttaaagcc gcttccggcg cgtgatctag ttcgccaagt
      481 gcccgatcca tatcccgaac acattcccgc cccatttcgt gctccgccaa catgaccccc
      541 gtcgggccac cctggcgggg aaaacccctg gcttctaaag ccggaaagag ccgatcctcc
      601 tccttagcgt gatggcaacg gtcggcaaag tgtcggataa aatcgatcac ctgacgtgcg
      661 gattcgacat cgaccgattt gctcgcctcg gcctgggtgg ccatcttttc cagcacggcc
      721 aaaacctgct caatcactcg atgttctgct cgcaagatat cggtaggttt catttgatcc
      781 tctccttatc aaggtcaact aaactgcaac acccagaatc gttcagtgaa acgccttgcg
      841 ttccaaaaac ggcactcggt ctgcccgcag ccccaaaacc cacgggaagg tttttggctc
      901 tttggcgaga ctccgcgatc acggtttgcc aagccttgtt ggctggttta taagtgaatc
      961 gcatgtggtt atcctattca atctcaaaaa aactgaacac ctttaccaca cctcgactta
     1021 acatcatgat ctacagcttg gcgactccct tcgttgcgcg accaattcat caagcgttgt
     1081 tgtccgcaag aattccagat agacggcccg caccttggcc catcgtacat ggacggggca
     1141 aggggcctca tcggaacagc gagatcgtcc taacaaacaa ccctcccatc ggctgacgcg
     1201 atccaggggc tcgacaacgt cgtagatggt gatcctttct ggtggacggg ccaaacgaaa
     1261 tccgccgcct tttccccgct gggattccaa taatccctcg tccgccagcc gctgcaggag
     1321 cttgcccagg taattgcgag gggccttgat ttgcttggcc agttccgcgg ttcccgcata
     1381 ttggtccggc ggcatttctg ccagaatcat taacgccctc agtccgtgga ttgctgttcg
     1441 ggagatcatg tcaaccttaa tttatggata atcacatgtg attatactta tatttcgaac
     1501 tctgtcaagt caaaaattcg ccgcggcata cgtccttccg ggttctagcc cgggcaacca
     1561 cagggggttg cccctaccaa ttgatggtcc gctcctagag gcccatcgaa cgtcccggcc
     1621 atagctccaa atgggacgtc aatgggcaac gtggtggacg ccggcgcgcc gctttagatt
     1681 tgggccagga aagcgcgttc ctccaaacaa tcaacgaacg ccctcgccca tgctgtgtgt
     1741 tggacgtgct accgccggga ggtaaaatcg gaggatgctg tatgttgcgt atcacctcga
     1801 tcacacgtca atc
//
LOCUS       DAOPTR010000871         1808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_50872_length_1808_cov_2.830006, whole genome shotgun sequence.
ACCESSION   DAOPTR010000871 DAOPTR010000000
VERSION     DAOPTR010000871.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50872_length_1808_cov_2.830006"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PKI05_14005"
     CDS             complement(<1..633)
                     /locus_tag="PKI05_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008672643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA53364.1"
                     /translation="MVCRSSIFCALVISLLSCPMASVAEEKGHPNIILVFIDDMGWGD
                     FSCFGNTEANTPNVDAMAAEGIRFFQFYVNSPICSPSRCAISTGQYPARWRITSYLNN
                     RADNARRGVANWLDPAAPMLARILQAHGYATGHFGKWHLGGQRDVDDAPPITAYGFDE
                     SLTNFEGMGPKLLPLTQKPGQQGYGRIWADAEVLGGPVIWMPRSQITGGYV"
     gene            984..1067
                     /locus_tag="PKI05_14010"
     tRNA            984..1067
                     /locus_tag="PKI05_14010"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1018..1020,aa:Leu,seq:gag)
     gene            1238..1311
                     /locus_tag="PKI05_14015"
     tRNA            1238..1311
                     /locus_tag="PKI05_14015"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1272..1274,aa:Lys,seq:ctt)
     gene            complement(1394..>1808)
                     /locus_tag="PKI05_14020"
     CDS             complement(1394..>1808)
                     /locus_tag="PKI05_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53365.1"
                     /translation="SCGTMWSDYVSDGKDSWGVFDPQGREEVFRLIEEHRIGGVLLIS
                     GDRHGARGFRIPRPGGYEFYEFEGASLGGRSGPPVRQPNWTTQLYGISGEYAFSEFEF
                     DVTKPDPEVTFRLISENGKTIYEITLPRSRLTLAP"
BASE COUNT          390 a          541 c          495 g          382 t
ORIGIN      
        1 cacataaccg cccgtaattt gcgaccgcgg catccaaatg accgggccac caaggacttc
       61 ggcatcggcc caaatccgac catatccttg ctggccgggt ttttgcgtga gcgggaggag
      121 tttcggtccc atcccctcaa agttggtcag tgattcgtca aacccatagg cggtaatggg
      181 cggggcgtca tccacatcgc gctgtcctcc aaggtgccac ttcccaaagt gccccgtggc
      241 gtaaccgtgg gcttgaagga ttcttgccag catcggcgcg gcgggatcga gccagttcgc
      301 cacaccgcgg cgggcattgt ctgcccgatt gttgagatag gatgtgatcc gccaccgtgc
      361 cggatattgc cccgtgctga tggcgcaccg agagggggag caaatcggcg agttgacata
      421 aaactggaaa aagcgaatcc cctcggcagc catcgcatcg acgttcgggg tgttcgcctc
      481 tgtattccca aagcaggaga aatcgcccca gcccatatca tcaatgaaca cgaggatgat
      541 attagggtgg cccttctcct cggccacact cgccatcggg caagataaca agctgatgac
      601 caatgcgcag aatatcgacg atcggcacac catgagtcgt tcctcccgcg aaaatcaacc
      661 cgatcggtta ggcgtcatag ctcaccataa ccttatccca tctcgttttc cccgtccaca
      721 cgagggaacc gcggttgctt tttcacccca tcaatgggca ggcacgagct ccgaccgcaa
      781 ccgggccatg tccgtcacgg acttgagacc cgtggcaagc cgggggtact actcgatcca
      841 cgatacctgc tccaattcgc cgacaaatgg tcaggtggac aacccgccag acggctcccg
      901 acgggtatca gggacttgac gttcaccacg tcaggttgct agattaaaag caaaaggtcc
      961 taaagacatt ctgtaccaac tctgcgggaa tggcggaatt ggcagacgcg ctaggttgag
     1021 ggcctagtgg ccgcaaggcc gtggaggttc aagtcctctt tcccgcactt ttgttttttt
     1081 ggtgatgata gttcgccgtt cgacgtcgtt cgtccagcgt accgtcaagt tttgctgcga
     1141 attttttgaa ctcaccgtag tcggtggcca gggatttgac ggttcagctt gaccgtacgg
     1201 tcgtcggatg gcaacggaat ggaaatcaac acaagccgcc cccttagctc agcccggcag
     1261 agcagctgac tcttaatcag cgggtcgtag gttcaaatcc tacagggggc atttgaacga
     1321 ttactcgaac atccattccg gaacgcccgc gttgccccct cacctccacc cgggcggcca
     1381 caggggcctg cctctacggt gcgagtgtta atcggcttcg tggtagggtg atctcgtaaa
     1441 tcgtttttcc gttttcgcta atcaatcgga acgtgacttc cggatcgggt ttggtcacat
     1501 cgaactcaaa ctcgctgaac gcatattctc cggagatgcc ataaagttgg gtggtccaat
     1561 tgggctggcg gacgggtggc ccactgcgac cgcctaggct tgccccttcg aactcgtaaa
     1621 actcgtagcc accaggtcgg gggatgcgaa acccacgggc accgtgtcga tcgccggaga
     1681 tgagcaggac cccgccgatc cgatgctcct cgatcagacg aaagacttcc tcccggcctt
     1741 gtgggtcgaa cacaccccag gagtccttgc catcggacac gtagtcactc cacattgtcc
     1801 cacaagag
//
LOCUS       DAOPTR010000872         1806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_50956_length_1806_cov_3.101085, whole genome shotgun sequence.
ACCESSION   DAOPTR010000872 DAOPTR010000000
VERSION     DAOPTR010000872.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50956_length_1806_cov_3.101085"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..1020)
                     /locus_tag="PKI05_14025"
     CDS             complement(250..1020)
                     /locus_tag="PKI05_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53366.1"
                     /translation="MYPPILLAVMCSSALLGQVAYSPWVETRSVRGLTIYSQFPLQDV
                     ADVQTDLEYLAEDIETALGLRLADRPVCVLLFAGRREYDRFLKERFPDVPYRRALYIQ
                     RSGQAFVLAYRSSELAVDLRHECTHALLHSALPVVPLWLDEGLAEYFEVPRAQRAFGH
                     PHLKTIRLWANLGYVPSLSTLEAIPHMDAMDANKYRWSFAWVHFMLHGPPAARLELVE
                     YLQTLAQYGVPRPLSERLAARLGDPRRAYLDHFKHWTR"
     gene            complement(1042..>1806)
                     /locus_tag="PKI05_14030"
     CDS             complement(1042..>1806)
                     /locus_tag="PKI05_14030"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53367.1"
                     /translation="CHLLPGLDDGPANWDQTLTMARAAVEHGLVWVVATPHQGGRYTE
                     NTPARIQSVVQEAKRKLEEHQIPLQLFAGADVRIDETLVERVQNKEVQPIADGRYLLL
                     ELPHDVYFPLQGLLNDLRRLGITGILTHPERNHAIIRRPELLNEILQAGALVQITAGS
                     ITGAFGAVVRRFSEELLLSGKVHVVATDAHGRHGRPPHLGDAHRQIARLLGRDTADIL
                     CRRNPARIVLGLAPQKLEPVRPIRSLWSRAWRRLAG"
BASE COUNT          382 a          523 c          572 g          329 t
ORIGIN      
        1 ggattgatcg tccattcagt gaatccggtt ttgctatggg gaaatgtccg ggtcctctgc
       61 cgccggcgtc acaccgcgat caacgaaggg gcttcgggag ggccagacgc gggtgaagtt
      121 gcctcaccgg caagtcggac caaactcgta cattatgtca tcctataccg gatgacaccc
      181 tgcggtaagg cgtattggag caaaggggcg gtcaatccga tgcttacaga gcagccgaag
      241 tcaagcggcc tagcgggtcc agtgtttgaa gtgatccaga tatgcccggc gggggtcacc
      301 caaacgggcg gcgagtcgct cgcttaaggg gcgtgggaca ccatattggg cgagggtttg
      361 aaggtattcc accagttcca gccgggcggc gggtggccca tggagcatga aatgtaccca
      421 cgcaaaggac catcggtatt tgttcgcgtc catggcgtcc atgtggggga tggcctctag
      481 tgtggaaagc gacgggacat aaccgagatt ggcccacagc cgtatagtct tcaaatgggg
      541 atgcccgaag gcccgttgag cacgggggac ctcgaagtac tcggccaatc cttcatccaa
      601 ccacaggggc accactggca aggctgagtg aagcagggcg tgcgtgcact catgacgtag
      661 atcaacggca agttcactgc tgcgataggc caggacgaac gcctgacccg accgttggat
      721 ataaagcgcc cgccgatagg gcacgtcggg aaaccgctct ttgaggaagc ggtcgtactc
      781 gcggcgtccg gcaaagagaa gcacgcagac cggcctgtcg gccagacgga ggcccagggc
      841 ggtctcgata tcctcggcca ggtattccaa atcggtttgg acgtcggcaa cgtcctggag
      901 cgggaactga gagtagatcg tcagcccccg caccgagcgt gtttccaccc agggcgagta
      961 cgccacctgg ccaagtagcg ccgaggaaca catcacggca agaaggatgg gcggatacac
     1021 ggcagtggct cccggccctt atcatccggc aaggcgtcgc cacgcccggc tccacagaga
     1081 acggatggga cgcacgggtt ccagcttttg gggtgccagc ccgaggacaa ttcgagccgg
     1141 attgcggcgg catagaatgt cagccgtatc tcgaccaagc agccgggcaa tctggcgatg
     1201 ggcgtccccc aagtgcggtg gacggccgtg acgcccatgg gcatccgtgg ccacgacgtg
     1261 gacctttccc gaaaggagta actcttcgct gaatcgtcgc acgacggccc caaaggctcc
     1321 cgtgatactc cccgccgtga tctgaacgag ggccccggcc tggaggattt cgttaagaag
     1381 ctccgggcgg cgaatgatcg cgtgattgcg ttcaggatgg gtcaagatgc ccgttatccc
     1441 cagccgtcgc aaatcgttga gaagcccctg caaaggaaaa tacacatcgt ggggtagttc
     1501 cagcaggaga taccggccat cggcaatcgg ttgcacctct ttgttttgga cccgttcgac
     1561 cagggtctcg tcgatgcgga catcggcccc agcaaaaagc tgaagaggaa tttggtgctc
     1621 ctccaacttg cgtttggcct cctggacaac gctttgaatg cgtgcaggcg tgttctcggt
     1681 atatcgccct ccttgatgcg gcgtggccac cacccacacc agtccgtgtt ccaccgccgc
     1741 ccgggccatg gtcagcgttt gatcccagtt ggcgggtcca tcgtcaaggc ccggcaaaag
     1801 gtgaca
//
LOCUS       DAOPTR010000873         1805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_50997_length_1805_cov_2.617143, whole genome shotgun sequence.
ACCESSION   DAOPTR010000873 DAOPTR010000000
VERSION     DAOPTR010000873.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50997_length_1805_cov_2.617143"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /gene="glgB"
                     /locus_tag="PKI05_14035"
     CDS             <1..1076
                     /gene="glgB"
                     /locus_tag="PKI05_14035"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HOA53368.1"
                     /translation="NYGRYEVRNFLIANALFWFDKYHIDGIRVDAVASMLYLDYSRGP
                     GEWIPNEFGGRENLQAIAFLKLLNEQVHLQFPGALTIAEESTAWPGVSRPTYQGGLGF
                     SMKWNMGWMNDTRTYMRKDPIYRKYHHDQLTFSLIYAFHENFVLPLSHDEVVHGKGSL
                     IDQMPGDWWQKFANLRLLYSYMWTHPGKKLLFMGSDFGQWHEWNCDGELQWELLDWPT
                     HRGLQQCVADLNHLLRREKALHEVDFNWEGFQWIDCHNWEESTLAYIRRAKDPSDYLV
                     IVCNFTPVPRWEHRIGVPEVGRFDEIFNSDAQLYGGGNIGNCGGVVSEPIPWHGFPAS
                     MKIVLPPLAAVIFKPRRDQGTTA"
     gene            1550..>1805
                     /locus_tag="PKI05_14040"
     CDS             1550..>1805
                     /locus_tag="PKI05_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008667966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOA53369.1"
                     /translation="MSAVVSVRRPRQGFTLVELLVVIAIIGILIALLLPAVQAAREAA
                     RRSQCTNNMKQLALAMHNYHDVYKIFPRSGYGGNDAGRPWN"
BASE COUNT          368 a          465 c          542 g          430 t
ORIGIN      
        1 tcaattatgg tcgttatgag gtgagaaact tcctcattgc caacgccttg ttttggtttg
       61 acaagtacca catcgacggt atccgggttg atgccgtggc ctcgatgctg tatttggact
      121 acagccgtgg acccggggag tggattccca atgaattcgg tgggcgagaa aatctccagg
      181 ccatcgcctt tctgaagttg ctcaacgaac aggtccatct gcaatttccg ggggccttaa
      241 cgatcgccga ggaatccacg gcttggccgg gcgtttcgcg gcctacttat cagggcggac
      301 tcggcttcag catgaagtgg aacatgggct ggatgaacga tacccgcacc tacatgcgga
      361 aggaccccat ctaccgcaag tatcatcacg accagttaac attcagccta atctacgcct
      421 ttcacgagaa tttcgtcttg ccactctcgc atgatgaggt tgtccacggg aaaggctcgt
      481 taatcgatca gatgccgggg gactggtggc agaagtttgc caacctccgc ttgctgtaca
      541 gctacatgtg gacgcatccc ggcaaaaagc tcctttttat gggcagcgat tttggccagt
      601 ggcatgagtg gaattgtgac ggagaacttc agtgggaact gctggattgg ccgacgcatc
      661 gtggcctgca acagtgcgtg gcggatctga atcacctctt acgccgagaa aaggccctgc
      721 acgaggtcga tttcaattgg gaaggcttcc agtggattga ctgccacaat tgggaagaga
      781 gcaccctggc atatatccgt cgggcgaaag acccttccga ttacctggtc atcgtttgta
      841 atttcacgcc ggtcccgcga tgggagcacc ggatcggtgt ccctgaggtc ggccgttttg
      901 acgaaatctt caacagcgat gcccagctct atggcggtgg aaatatcggc aactgcggtg
      961 gggtggtatc cgagccgatc ccctggcacg ggttcccggc gtcgatgaag attgtcctgc
     1021 cccccttggc ggccgtcata ttcaaacccc ggcgggatca gggcacgacc gcgtgatgag
     1081 cctcgctgac ggcgccccgc aagggtgaca caaagccggg agggaacgcg gtctcccgcg
     1141 tccgcccgtt ccggggagac gtgctgtgtg gcgtccgtcg tctttccggt aaggcgttgg
     1201 gattggcctt gaccagcatg gcgcgtggcc ggggagtttt tttggtttgc acaagggcgc
     1261 gcaatcttct atgatgaaat gagagcactt gacggtgggt gtaccacccc ggctgcttcg
     1321 ccaagaaggc cgagggaggg gtaattggtg gcttccttcc gtcttctggc ctccttaggg
     1381 atatttccat ttaagtttct ggagcagcca tttctttcgg tcagtaacag gaacaaacca
     1441 aatcgttgac aaaaaaggcc gcgaaaacta gactaccaat gtggctgtat gtcatgtcac
     1501 gtggtgctgt atcttttgat tcagttgttt gcttgtggag gatgaggtta tgtcagcagt
     1561 tgtctctgta aggcgtcctc gccaggggtt taccctggtg gaacttcttg tggtaattgc
     1621 catcatcggt atcctcattg cgttgctgct ccctgcggtg caggcggcgc gggaggccgc
     1681 gcggcggagt caatgtacga acaacatgaa gcaactcgcg ctggccatgc acaattacca
     1741 cgacgtttat aagattttcc cgcggtcggg ctatggtggg aatgacgcgg ggcggccgtg
     1801 gaatg
//
LOCUS       DAOPTR010000874         1802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_51097_length_1802_cov_2.663995, whole genome shotgun sequence.
ACCESSION   DAOPTR010000874 DAOPTR010000000
VERSION     DAOPTR010000874.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51097_length_1802_cov_2.663995"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            352..618
                     /locus_tag="PKI05_14045"
     CDS             352..618
                     /locus_tag="PKI05_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53370.1"
                     /translation="MRQGVLLIAVVLALAVGTMSNAFANSRQQYREAIRSMPLLERPN
                     RFGHIYGNTVRRIYHRRYGSLPGQQTTVPENGSHLVLPMPTPVQ"
     gene            894..1130
                     /locus_tag="PKI05_14050"
     CDS             894..1130
                     /locus_tag="PKI05_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53371.1"
                     /translation="MRRAGVQVLDPVLDHCQVEWLLVSADECPDQETEAWIIRTHFRI
                     PQTPWGQHKAYVKPVEVRRSRRWVLFRQVSGILD"
     gene            complement(1482..>1802)
                     /locus_tag="PKI05_14055"
     CDS             complement(1482..>1802)
                     /locus_tag="PKI05_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53372.1"
                     /translation="FLAGLALLGLFLAVVTGCPAGGTPKPKEYGIPMKTPLEEAKSLL
                     ENYAAGGSIGSEAASYPDLVNAVRQSDPAKADILEKGFAELQKTPPQGVAAKAKEILA
                     QLGQ"
BASE COUNT          366 a          553 c          500 g          383 t
ORIGIN      
        1 aggagctgga gtggcggatc gacgagctcg ccgcccagga ggagctggac gcgaaggaat
       61 atcaggcgac ttcgggcggt ttgggaccgg gctcaatgcg cgccaaacct gcacacaccc
      121 cgccatcttg tcgtaaataa tttaggctac ctgaacgagc tgcgtccgac aaccgcgggt
      181 gccgaccttc cgcttttttc gctaaaatcg ccattttcct tcgtgggaaa aacctcccct
      241 gtgttgacat ccgatgccta gacaatggag tgagatcgtg gcaaggatgc gtaggtaccc
      301 ctcttaggat gatctttgca gctgaagcac ctttgaaagg aggcccctac gatgcgtcaa
      361 ggagtcttgt tgattgccgt tgtcctggcc ttggccgttg gcacaatgag caacgccttc
      421 gcaaatagcc gacaacagta ccgagaagcc atccgctcga tgccactttt ggagcggccc
      481 aaccgctttg ggcacatcta cggcaacacc gttcggcgga tctaccaccg acggtatggc
      541 tccttaccgg gtcagcaaac gaccgttccc gaaaacggct cccacctggt gctacccatg
      601 ccgacccccg tgcaatagcc accccccacg ggttactttc ctggttcaca ggcgttacgg
      661 acaagccgct tggcgcgccc cgctgttgtg ttgcggtcaa gcaacgcaga ggacacgtcg
      721 ctgacgtgaa atgacatctc cagctcgctc tggtccttgg gattgaggcg gtcttggccg
      781 cgggtcttgg ctgtcccgtc aggatcacct caattggtac ggcattcgct tgagcttctt
      841 ctgccattga ggctcaccac cgatcccgtc ccatggaaga aggagtgaaa gcgatgcgac
      901 gagctggtgt gcaggtcttg gacccggtgt tggatcactg ccaggtcgag tggttactgg
      961 tctcggcgga cgaatgcccg gaccaagaaa ccgaagcatg gattatccgc acccatttcc
     1021 gcatccccca gaccccctgg ggacagcata aggcctacgt caaaccggtg gaagtccgtc
     1081 gctcccgacg ttgggtcctc ttccgccagg tctccggcat cctcgattag gatcaaccct
     1141 aggacgaaca aaaccagcgg cgtcgtccgc ctttcctcgg tcggtttgcc gagttgtctc
     1201 aattgctgcg caagaatttg cagcacaaga aacacacaaa aggccgaaga ttaccggcgg
     1261 gggtcaatcc tcggcctaac aaatgccgca cggacagaaa aacgttttgg gcgttcttag
     1321 gccctgtcca acgacctcgc ctctcccgtt cggcgggaga ggggccgggg gtgagggtga
     1381 ctcgaatcgc tgcccgtttt ctcgatcgac tgcgaccgtc gcttgccctc accctaaccc
     1441 tctcccagag ggagaggaaa ctttgagata ggcccttcta actattggcc cagttgtgcc
     1501 aagatttcct tggccttggc tgccacccct tgcggcggtg tcttttgcaa ttcggcgaag
     1561 cctttctcca ggatatcggc tttggcgggg tcgctctgcc gaactgcgtt cacgaggtca
     1621 ggatagcttg cggcctcact accgattgaa cctccagcgg cgtaattctc caacaggctc
     1681 ttagcctcct ccagcggggt cttcatggga atgccgtatt ccttgggctt cggggtgcca
     1741 ccggcagggc agccggtaac caccgccaaa aacagtccca acagtgccaa tcctgccaaa
     1801 aa
//
LOCUS       DAOPTR010000875         1800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_51176_length_1800_cov_2.797135, whole genome shotgun sequence.
ACCESSION   DAOPTR010000875 DAOPTR010000000
VERSION     DAOPTR010000875.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51176_length_1800_cov_2.797135"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="PKI05_14060"
     CDS             complement(<1..1277)
                     /locus_tag="PKI05_14060"
                     /inference="COORDINATES: protein motif:HMM:NF014649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53373.1"
                     /translation="MVHVSRRKPLFARVGVFGVGHGTYWGQFPGLLDVMHQKLEKLCQ
                     KLAAYGVEVINFGLVDNAQGAYALLPRLQAADLDLVFCDMVTYATSSTFGVIIRNLSI
                     PLVLVALQPLAALDYPRATTFMQLCNDDFCAVPEFAGVAVRMGKRVPPLILGMLENDP
                     VADAELATWCQIAKVLRDLRTARIGLMGHVLEAMLDMHSDPTALTAAFGCHIVQVEPD
                     DLMRHHRDIPESLITEKIQEILNLFDTPEPQSDPITHKASAEDLRAAAKAAVALDRLI
                     EEKSLDGLAYYYEGEPGSPMRQLMANLIVGNSLLTAAGFPMCGEADLKTCIAMLILDR
                     LEAGGSFAEFHPIDFREGFVLVGHDGPHHINIAEGKPVLRSLDRYHGKPGSGVSVEFK
                     IREGPITLLGITQTNDGRCKFVVAEGQSIKGPIP"
     gene            complement(1300..>1800)
                     /locus_tag="PKI05_14065"
     CDS             complement(1300..>1800)
                     /locus_tag="PKI05_14065"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53374.1"
                     /translation="GVVLAGLFAAVMSTLDSGINSLAAVVAYDWLGGQKLSVTGSRLL
                     TLVFGATIVAAAVVVPAIAEHVIDIIAGIAGTFLGLLLGLFVLGMFFPRANTLGAAIG
                     LFAGIICWPGRSRQRAFRIGGMEPSRVSPSLWWVISPVALRPHQTPIKPVASPSERKK
                     GVRSRA"
BASE COUNT          443 a          519 c          484 g          354 t
ORIGIN      
        1 ggaataggac cttttatgga ttgaccctca gcaacgacaa atttgcagcg accatcgttt
       61 gtttgggtga ttccgagaag tgtgatgggg ccctcacgaa tcttgaactc cacgctgacc
      121 cctgaccccg gcttcccgtg atagcgatcg aggcttctca atacgggttt gccttcggca
      181 atgttaatat gatgcggacc atcgtgtccc acaaggacaa agccttcgcg gaaatcaatg
      241 ggatgaaatt cggcgaaact gccgccggcc tccaaacggt ccaaaatgag catggcaata
      301 caggtcttca ggtcggcctc gccacacatg ggaaaaccgg ccgccgtcaa tagggagttg
      361 ccaacaatca agttggccat taactgccgc atcggacttc ccggttctcc ctcgtagtaa
      421 tatgccaggc catcaagtga tttttcctca atcaagcggt caagcgcaac ggcggccttt
      481 gcggcggcgc gcagatcctc tgcgctcgct ttatgggtga tggggtctga ttggggctcg
      541 ggagtgtcaa agaggttgag gatttcctgg attttttccg tgatcagact ttccgggata
      601 tcgcggtggt gtcgcatcaa atcgtctggt tcaacctgca caatgtgaca cccgaaggcc
      661 gcggtaagag ccgtggggtc actgtgcata tctaacatag cctcgaggac atgacccatt
      721 aggccgattc gtgctgtgcg caaatcgcgc aggactttgg caatttgaca ccacgttgcc
      781 aactcggcat ccgccacggg gtcgttctcg agcatgccga ggatcaaagg cggtacccgt
      841 ttgcccatcc gcacagccac acctgcaaac tcggggactg cacaaaagtc gtcattgcac
      901 agttgcataa acgtggttgc tcggggatag tccagagcag ccaagggttg cagggctacc
      961 agaaccaagg gaatcgacaa gttgcgaatg atgacgccaa acgtggaact ggtagcgtag
     1021 gtcaccatgt cacagaaaac caggtcgaga tccgccgctt ggagtcgtgg aagaagggcg
     1081 tacgccccct gggcattatc gaccagacca aagttgatca cctcaacgcc gtaggccgca
     1141 agcttttgac agagcttttc cagcttctgg tgcatgacgt cgagcaaacc cggaaattgt
     1201 ccccaataag tgccgtgtcc cacgccgaag acgcccaccc gggcaaaaag aggtttccga
     1261 cgggaaacat gcaccatgga cgacctcgac gaactatttt cacgcacggc tcctaactcc
     1321 cttttttctc tccgatggcg acgccacggg cttgatcggc gtttggtggg ggcgcaaagc
     1381 aactggcgaa ataacccacc acaacgatgg ggagactcgc gaaggctcca taccaccaat
     1441 gcggaatgcc ctttgccgag atcgaccagg ccaacagata atcccggcaa acaatccgat
     1501 ggccgcaccg agggtattcg cgcgcggaaa gaacatgccc aggacaaaca agcccaggag
     1561 caaaccaaga aacgtccctg caattccggc aataatgtca atgacgtgtt cggcaatagc
     1621 cggcacgaca acggccgctg ccacgatggt cgccccaaaa accaatgtta gcaagcggct
     1681 ccctgtgacc gagagtttct gcccaccaag ccaatcgtaa gccacaactg ccgccaaact
     1741 gttaatgccg ctgtcgagcg tgctcatcac ggcggcaaat agtcccgcca ggactacgcc
//
LOCUS       DAOPTR010000876         1798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_51240_length_1798_cov_3.791738, whole genome shotgun sequence.
ACCESSION   DAOPTR010000876 DAOPTR010000000
VERSION     DAOPTR010000876.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51240_length_1798_cov_3.791738"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1798)
                     /locus_tag="PKI05_14070"
     CDS             complement(<1..>1798)
                     /locus_tag="PKI05_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53375.1"
                     /translation="AQDAPETQGSYVFATVSEENADAWIALAKQAGIAQIHMISWEQS
                     LGHYQPRKNLFPNGIEGLKQVVEKIHAAGLKAGMHTLTGCISPSDPWVTPKPDPRLAV
                     DVRFTLVDPLDEKADRIVTREKPGNLDTVWAYGGHGNVLRIGDELIQYSGLSQEPPYG
                     FTGCRRGAFGTQPQKHEAEAPVEHLFVRYGCFLPDENSTLVEEIAQAIADVYNTCGFD
                     MIYMDGAEGMPGGWYGISRMREEIFRRLKRPALVEASCWDYHSWAFHSRLGAWDHPNW
                     GLKPFVDWHCRSNEQHRRSSLLPVQLGWWAILGPTADHDAELPDEFEYLCAKSLAYGM
                     PMSFQGVAPGDNPPCARQPEYLEMLGRYEHLRLSNQVPPQIRERLKQEGQDFHLEVRD
                     DGSWAFRPADYVSHKITGAVPESTTWTVNNRYSAQPLRVRIQALYGVEPYDSANAEEI
                     VSFKEGEFSQVAQAAGVKAELSYVTDEIKVGSSSGKLVAENTRGTSRGAWAHFTKTFD
                     PHVNMQKRAAMGVWIHGDGSGALLNLQLTNPLHYWPTCDEHYVRLDFTGWRYVEFLLR
                     ERDADDFHRYEWPYGSYYAVVYRSPLIRDHVSA"
BASE COUNT          388 a          532 c          496 g          382 t
ORIGIN      
        1 ggcgctaacg tggtcacgaa tcagcggtga tcggtacaca acggcataat agctgccgta
       61 tggccattcg tagcggtgaa aatcatcggc gtcgcgctcc cgcaaaagaa actccacata
      121 gcgccagccg gtgaagtcca gccggacata gtgctcatca catgttggcc agtaatggag
      181 gggattcgtc aattgcaagt tgagaagcgc cccgctcccg tccccatgaa tccaaacgcc
      241 catggccgcg cgcttttgca tgttcacgtg gggatcgaat gttttcgtga aatgagccca
      301 cgccccgcgg gaggtgcccc gggtattctc ggcgacgagc ttgccactgc tcgaaccaac
      361 cttgatctca tcggtaacat acgatagttc tgctttaaca ccagcagcct gggcaacctg
      421 agagaactcg ccctccttga aagagacaat ctcctcagca ttagcgctgt catagggttc
      481 gaccccatag agcgcctgaa tgcgaacgcg gagcggctgg gcggaatatc ggttatttac
      541 tgtccatgtc gtgctttccg gcacggcgcc cgtgatttta tggctcacat aatcggccgg
      601 gcgaaatgcc caggaaccat catcgcggac ttccaaatgg aaatcttgcc cttcctgctt
      661 gaggcgttca cgaatttgcg gtggcacttg gttggagagc cgaaggtgct cgtatcggcc
      721 caacatttcg aggtactcgg gctggcgggc gcaagggggg ttatcacccg gcgcgacgcc
      781 ctgaaacgac atcggcatcc cgtaggccag tgacttcgcg cagaggtatt cgaactcgtc
      841 gggaagctca gcatcgtgat cggcggttgg ccccaaaatg gcccaccagc caagttggac
      901 cggtaatagc gagctgcgac gatgttgctc attcgaccgg caatgccaat ccacaaaggg
      961 tttcaagccc cagttgggat gatcccaggc cccaaggcga gaatgaaatg cccaggaatg
     1021 gtagtcccaa caactcgctt ccaccagcgc aggacgcttc aggcgtcgga aaatctcctc
     1081 tctcatgcgg ctaatcccat accaaccgcc gggcatgccc tcggcgccgt ccatataaat
     1141 catgtcaaag ccacatgtgt tatagacgtc ggcaatggcc tgggcgattt cctccaccag
     1201 cgtcgagttc tcgtccggca aaaagcaccc gtatcgcaca aacagatgct ccacaggggc
     1261 ctccgcttcg tgcttttgcg gctgcgttcc aaaggcacca cggcggcagc cggtaaaccc
     1321 ataaggtggt tcctgcgata agccgctgta ctggatcaac tcatcaccga tgcgaagcac
     1381 gttcccatgt ccaccatagg cccaaacggt gtccagattg ccgggcttct cgcgggtcac
     1441 aatccggtca gccttctcat cgaggggatc aaccaaggtg aatcgtacat cgacggccag
     1501 ccgcgggtcg ggcttggggg tcacccaggg gtctgacgga gagatgcacc cggtcagcgt
     1561 gtgcataccc gctttaagcc ccgctgcgtg gatcttttcc acgacttgtt taagtccttc
     1621 gattccgttg ggaaagagat ttttccgagg ctgataatgc cccaggcttt gttcccaact
     1681 gatcatgtgt atttgggcga tacctgcctg ttttgccaga gcgatccacg catctgcgtt
     1741 ttcttccgaa actgtggcaa atacatagga gccttgcgtc tcgggcgcgt cctgggcc
//
LOCUS       DAOPTR010000877         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_51323_length_1796_cov_2.864446, whole genome shotgun sequence.
ACCESSION   DAOPTR010000877 DAOPTR010000000
VERSION     DAOPTR010000877.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51323_length_1796_cov_2.864446"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PKI05_14075"
     CDS             <1..214
                     /locus_tag="PKI05_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53376.1"
                     /translation="VVEAMISLDQSYYIAHPWDVQVPDCIVDGASDSSYTDSAGMDQG
                     RLKTGDIDQDQGAIDGCGDDVEDPSL"
     gene            493..816
                     /locus_tag="PKI05_14080"
     CDS             493..816
                     /locus_tag="PKI05_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53377.1"
                     /translation="MKAVFQRILRSLHDDHGVLTFEWILLLTLVVIGIVGGLSAARDA
                     LISELGDVVGAVVHIDQSYTVSSDATGWGSEFSYSDELPECAGEQNRPTTGRPDQGPV
                     GECSQ"
     gene            1001..1234
                     /locus_tag="PKI05_14085"
     CDS             1001..1234
                     /locus_tag="PKI05_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53378.1"
                     /translation="MTFEWLILITLLVIGVIGAYSAVRDGMVDELGDTATAILSVDQS
                     FHTEPPERLPCAGSWGSWEDDDAAEQLERGRNL"
     gene            1369..>1796
                     /locus_tag="PKI05_14090"
     CDS             1369..>1796
                     /locus_tag="PKI05_14090"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HOA53379.1"
                     /translation="MFVAIKAFPILAIAVGVIAGWAAFTDLRQRRIPNFVTVPAALLG
                     LLFHSLLPSGEGWWFSLAGFAVGAVLLVLPYLLGGGGMGDIKLLAALGSWLGPFWILV
                     AFATGTVCGAVLTLICLVTGRSLEELLGISQGAHDDGKKPA"
BASE COUNT          391 a          460 c          516 g          429 t
ORIGIN      
        1 cgtggtcgaa gcgatgatct cgctcgatca gtcctactac atcgcccatc cgtgggatgt
       61 ccaagttcct gactgcatcg tggatggcgc cagcgactcg tcttacaccg actctgctgg
      121 aatggatcag ggtcgtctaa agactggcga cattgatcag gaccagggag cgattgatgg
      181 ctgcggcgac gatgtggaag atccaagcct gtgatctgcc cacgtaacat cgtctgacag
      241 catttccaga catcggtctc ggaagtgcgc ggctccgcgg aatcatcttc cgcggagcct
      301 tttttatatg ggatatggtg atggatgacc aagcgtaacc acgcaatcgc acttcgaaaa
      361 aatcaaagtt ccccagtacc agctcgttca aaatggcatg aaatctgcac atcaagcaaa
      421 gacaaacatc gcggggcaaa agttactcgt cgatttaaac acaactcttg tggaaaaagg
      481 tgaggaccgt ctatgaaagc cgtcttccaa agaatcttgc gatccctgca tgatgatcat
      541 ggtgttttga ccttcgaatg gattctcttg ctgaccctcg ttgtcattgg aattgtcggt
      601 ggtctctctg ccgcacgtga cgccctgatc agcgagctgg gggatgtcgt tggggccgtc
      661 gtgcacatcg atcaatcgta cactgtctca tccgatgcga cgggttgggg aagtgagttt
      721 tcttattctg atgaactacc cgagtgtgcc ggtgaacaga atcgaccaac aacaggccgc
      781 ccggaccaag ggcccgttgg tgagtgttcc cagtgaccca ccgggcaagg gtgtttccat
      841 tctgaaataa cctgatgttg cgtgttgcat ttgggaagca agctcgaagg aatgtgtggt
      901 aacgaaacgc gtggtttgcg attcctcatc ctggagacat aacatgggcc atcgcatccc
      961 ccgaaaaacc ggtgcaaccc gtcgtggaag acgcggttta atgacgttcg agtggctgat
     1021 tctgatcacg ctgctagtaa ttggcgttat cggtgcctac agcgctgtcc gcgacggcat
     1081 ggtcgatgag cttggagaca cagccacggc cattctttcc gtggaccaat cttttcacac
     1141 agaaccgcca gagcgtctcc cttgtgcggg gagctgggga agttgggagg acgatgacgc
     1201 cgcggaacag ttggagcgag gccggaacct gtaaagaatg gcatgacgca tgccttgttg
     1261 ctggatgagc ggagggtaag gcaggggcca aaacaaacgg ggtaacacaa accacaccaa
     1321 ccaccagggg catcagcttt tctcaacaac agtatgggac ttttcgaaat gtttgtggca
     1381 atcaaggctt ttccgatttt ggctattgct gttggtgtca ttgctggatg ggccgcgttc
     1441 accgacttgc ggcaacgacg gattcccaat ttcgtgaccg tgccagcggc ccttctcggg
     1501 ttactgtttc acagcttatt accgtctggt gagggttggt ggttctccct ggcaggtttc
     1561 gccgtggggg cagtgctcct tgtacttcct tatttgctcg gcggtggagg tatgggcgac
     1621 atcaaactac ttgccgccct aggaagctgg ctaggaccct tttggatcct cgtcgccttt
     1681 gccacgggga cagtctgcgg tgccgtcttg accctcattt gcctggtgac cggtcgctca
     1741 ctggaggaat tgctcggcat tagccagggc gctcatgatg acgggaaaaa gcccgc
//
LOCUS       DAOPTR010000878         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_51328_length_1796_cov_2.119472, whole genome shotgun sequence.
ACCESSION   DAOPTR010000878 DAOPTR010000000
VERSION     DAOPTR010000878.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51328_length_1796_cov_2.119472"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /gene="gap"
                     /locus_tag="PKI05_14095"
     CDS             complement(<1..1006)
                     /gene="gap"
                     /locus_tag="PKI05_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOA53380.1"
                     /translation="MAIRVGINGFGRIGRLVVRRMMQKAGQFQIVGVNDITDVKTLAM
                     LFKYDSVHRTYPGTVSFDEKNLIIDGQKIPVMAEKDPTKLPWKDLGADIVVESTGVFT
                     SRGGDGKPGYASHLEAGAKKVVLSAPAKDNPDLTCVLGVNDDQLKPEHKCVSNASCTT
                     NCLAPVAKVLHEKFGIVRGLMTTVHAYTNDQRVLDLPHKDLYRARAAAANIIPTSTGA
                     AKAVGLVIPELKGKLTGIAMRVPVVTGSVVDLVAELKRPVEAAEINAAVKEAAEGKLK
                     GILQYTEDPIVSSDVIGNPHSSIFAAPWTQVMEGSMVKVVAWYDNEWGYSCRTVDLVE
                     R"
     gene            1123..>1796
                     /locus_tag="PKI05_14100"
     CDS             1123..>1796
                     /locus_tag="PKI05_14100"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOA53381.1"
                     /translation="MRLLAAYRPTWLLPSGVPRGVWEYAHSEDIAAEYDESIASTDLV
                     VFDQQVIARYCKLPGLVVDLGCGTGRALIPLLKRGFRGVGVDLSRPMLQAFTEKAKQN
                     GVSVWAIHANIVELDCLQDGIADYCLCLFSTIGMIHGRSNRRRVLEHVRRILKPGGVF
                     IVHVHNVWFQALTAGGRSWLIPHWFQTRILRKGQFGDRFFEYHGIPNVFVHAFTRREF
                     LRDLRSA"
BASE COUNT          372 a          498 c          478 g          448 t
ORIGIN      
        1 gccgctccac cagatctacc gttcggcaac tgtaacccca ctcgttgtcg taccacgcca
       61 cgactttcac catcgaacct tccatgacct gcgtccaggg agcggcaaag atcgagctgt
      121 gtggattgcc aataacgtca ctggagacga tggggtcttc cgtgtattgg aggatcccct
      181 tcagtttgcc ttcagcagct tccttgaccg ccgcattgat ttctgcagcc tccacgggac
      241 gctttaactc ggccacgagg tccacaacac ttcccgtaac caccggcacc cgcatggcga
      301 taccggtcaa tttccctttt agttccggaa tgaccaatcc aacggccttt gccgcacccg
      361 tcgaggtcgg aataatgtta gctgctgcgg cccgggcccg ataaaggtcc ttgtgaggca
      421 aatccaacac gcgctgatcg ttcgtgtacg cgtgaacggt cgtcatcagg ccccgcacga
      481 taccgaactt ctcgtgcaac accttggcga caggtgcgag gcagtttgtc gtgcaactgg
      541 cgtttgaaac gcacttatgc tcgggtttca gttggtcatc attgaccccc aaaacgcacg
      601 tgagatcggg attgtcctta gctggagcac tcaaaacgac ctttttggcg ccggcttcaa
      661 ggtgagaagc gtaaccgggc ttgccatctc cgccgcggct tgtgaagacg cccgtgctct
      721 cgacgacaat atccgcaccg agatccttcc aaggcagctt ggtcggatcc ttttctgcca
      781 tgacggggat cttttggcca tcgatgatga gattcttttc atcgaagcta acggttcccg
      841 gataggtccg atgcacactg tcatacttga agagcattgc gagcgtcttg acgtcggtaa
      901 tgtcattcac gccgacgatt tgaaattggc ccgccttctg catcatccgg cgaactacga
      961 gccggccaat ccgcccgaaa ccattgattc caactcgaat agccacggct ttctcctttc
     1021 ccttaaagca accacagaac gcgtattgtg ttcgtcttcc gcagagttaa aaggcttatt
     1081 atattggaca atgttactaa tgacaaccac cgggagcgtg acgtgcgatt actggcggct
     1141 tatcggccaa catggcttct tccaagcggt gttccaagag gtgtctggga atacgcccat
     1201 tccgaagaca tcgcggcgga gtatgacgag tcgattgcct ctaccgacct ggtggttttc
     1261 gatcagcaag tcatagccag atactgtaaa ctcccggggc ttgtggtaga tttgggctgt
     1321 ggaaccggcc gggcgttgat cccgctcctt aagcggggtt ttcgtggggt tggtgtcgat
     1381 ttgtcgcgtc cgatgttgca agccttcacg gaaaaggcca agcagaatgg tgtgagtgtt
     1441 tgggcaattc atgccaacat tgttgagctg gattgccttc aagacggtat tgccgattac
     1501 tgcctgtgtc tgtttagcac gatcggaatg attcacgggc gatcaaatcg ccgccgtgtt
     1561 ttggagcatg tgagacggat tctcaagccc ggcggggttt ttatcgtgca cgtgcacaat
     1621 gtatggtttc aggccttgac cgccggggga cggagttggc tcatcccgca ttggttccaa
     1681 acgcgcatcc tgagaaaagg ccaattcggc gatcggtttt ttgaatatca cggcataccg
     1741 aatgtgtttg tccacgcctt tacacgccgc gagtttctgc gtgatctccg ttctgc
//
LOCUS       DAOPTR010000879         1795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_51354_length_1795_cov_3.263218, whole genome shotgun sequence.
ACCESSION   DAOPTR010000879 DAOPTR010000000
VERSION     DAOPTR010000879.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51354_length_1795_cov_3.263218"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(315..>1795)
                     /locus_tag="PKI05_14105"
     CDS             complement(315..>1795)
                     /locus_tag="PKI05_14105"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOA53382.1"
                     /translation="DPTCSPTRAALMTGRYSCRTGVWHTIMGRSILRRDETTMADVFR
                     RAGYRTAIFGKWHLGDNYPYHPWNRGFEECLIHFGGGIGQGPDYWGNTYFSPTLNHNG
                     EWKKEEGYCTDIFFKRAMEFIQAHREEPFFVYLPTNAPHAPLQVEERYAEPYRQAGLP
                     DALAKFYGMITNIDENMGRLMGLLRELKLESQTILIFMTDNGTAGSGFNAGMRGKKGS
                     QYDGGHRVPCFIRWPGRLPAGQDFTHLAAHIDLFPTLLDLCDIPAPSEVKFDGISLKP
                     YLAGGTATFPERTLFVQVNRIDHPVPWRLSAVLTERYRLIDGQQLYDIKEDPGQTKDI
                     AAANAEVVNELRARYEAWYADVSQRFGEYCELVLGSDEENPATLCSHDWHGESVPWDQ
                     SQMEKGMVGNGFWAVEFAKAGRYRFALRDRPAHASTKLPQGKARLVVEGLFEEEKPIP
                     ANAEEIIFEANLPAGKARLQTFLTLPNGTQRGFYFVVVTRLP"
BASE COUNT          396 a          518 c          491 g          390 t
ORIGIN      
        1 ctcctcgatg cggaaccagt cgtcggcggc gcgctcggcg atcgccacca gggtaggtac
       61 tatggagcgc tcggcccaag agaaaagcca catgacgagc gccaaaatcc caatgaggta
      121 gactgccgcc ctggtacgtc gcccaatgcc aaatattgca gcatgtgtgg gcctaagttt
      181 tgctcgatgc ggctctcgca agatgtggcc caatcgcaac agtcagccaa tgatccccag
      241 ccttcccatg tcccggggga cgcttaagct caccagccgt gacaccttgc agtttgaaca
      301 ctcggcaacg gctgtcaagg tagtcgagta acgaccacga aatagaagcc tcgctgggtc
      361 ccattaggaa gcgttaggaa tgtttgcagg cgggccttcc ccgcaggcaa attggcctca
      421 aagatgattt cttcggcatt ggctgggatt ggcttttctt cttcaaacag tccttccacg
      481 accaagcggg cctttccctg gggcaacttg gtggaagcgt gggccgggcg atcgcggagg
      541 gcaaaacgat atcgcccggc cttggcgaat tccacagccc aaaagccgtt gcccaccatc
      601 cccttctcca tctgcgactg gtcccatgga accgattcgc cgtgccaatc gtgggaacac
      661 aacgtggccg ggttttcctc gtcggatccg agcaccaatt cgcaatactc gccaaatcgc
      721 tgggagacgt ccgcgtacca ggcttcgtac ctcgcccgaa gctcattcac gacctcggca
      781 ttggccgccg caatatcctt tgtttgtccg ggatcctcct tgatgtcgta aagctgttga
      841 ccatcaatca accggtatcg ctcagtcaga acggcagaca atcgccaggg aaccggatga
      901 tcaatacggt taacctggac gaataatgtc cgctcgggaa acgtggcagt tccgccggca
      961 agatacggtt tgaggcttat cccgtcaaat ttcacttcgc tgggggccgg gatgtcgcac
     1021 agatcaagca gtgtggggaa aaggtcgatg tgtgccgcca agtgggtgaa gtcttggcca
     1081 gccgggagac gtcctggcca gcggatgaaa caggggacgc gatgaccacc gtcgtattgg
     1141 gagcctttct ttccccgcat tccggcgttg aaaccactcc cggcggtccc attatccgtc
     1201 atgaagatga gaattgtttg ggattccagt ttcaattctc gcaatagccc cattagtcgc
     1261 cccatattct cgtcgatgtt ggtaatcatc ccatagaatt ttgccaaagc gtcaggcagc
     1321 ccggcttggc gatacggttc agcatagcgc tcttcaacct gaagcggggc atgaggcgca
     1381 ttcgtgggaa gatagacaaa aaacggttct tcgcggtgcg cctggatgaa ctccatggcc
     1441 cttttaaaga agatgtccgt acagtaacct tcctccttct tccattcacc attatggttg
     1501 agtgttggcg agaagtatgt gtttccccaa tagtccggtc cctgaccgat gccacccccg
     1561 aaatggatga ggcattcctc aaatccccgg ttccatgggt gataggggta attgtctccg
     1621 aggtgccatt ttccaaagat agccgttcgg taccccgcac ggcgaaagac atccgccatg
     1681 gtcgtctcat ctcgccgcaa aatagaacgg cccatgatcg tatgccaaac cccggttcgg
     1741 caggagtaac ggcctgtcat caaggccgca cgggtgggcg agcacgtggg atcga
//
LOCUS       DAOPTR010000880         1792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_51468_length_1792_cov_3.779505, whole genome shotgun sequence.
ACCESSION   DAOPTR010000880 DAOPTR010000000
VERSION     DAOPTR010000880.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51468_length_1792_cov_3.779505"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..164
                     /locus_tag="PKI05_14110"
     CDS             <1..164
                     /locus_tag="PKI05_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-octaprenyl-4-hydroxybenzoate carboxy-lyase"
                     /protein_id="HOA53383.1"
                     /translation="NLALAAEAGATVLPAMPAFYHNVLTVRDLVDFIVARIIDQLGIE
                     NELIRRWGT"
     gene            295..1158
                     /locus_tag="PKI05_14115"
     CDS             295..1158
                     /locus_tag="PKI05_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011119017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HOA53384.1"
                     /translation="MRKVRHFLELIRFSHTLFALPFAALSAAMVWKVVPFQWHHLLGF
                     LLCMVFARSTAMAFNRLVDRKLDAANPRTAGRHIPRGVLGVREVWGFTAVCAIGFVLS
                     TLLFLPNYWPLVASGPVLAFLCGYSYSKRFTWLSHFWLGTALALAPPGIWLAIQGTLA
                     WPPILLACAVIFWVAGFDIIYACQDYDFDRRQKLWSIPAVFGIRRALWLAAASHGVMV
                     GCLAMLPILFNEFDWLYWVGLALVSVLLIYEHWLVRPDDLRRVNEAFFHVNAVVSVGL
                     LIIGTVDIWLL"
     gene            1183..>1792
                     /locus_tag="PKI05_14120"
     CDS             1183..>1792
                     /locus_tag="PKI05_14120"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOA53385.1"
                     /translation="MLDQNIVRTERLVESRLDEIRNKIEAGRRLSFEDGVFLFQPSVS
                     LHVVGQLADLVCRRRHGRTVYYNLNTHLNPTNICGYRCKLCAFFRSKGDPDAYIMSQE
                     EILKRAEDADQAGCTELHIVGGLPPGIPFEWYLGIVEAIHNQYPRLHLKAWTAVEIAW
                     FARISGLSVKEVLLRLIDAGLGSLPGGGAEIFHPEVRRQISSH"
BASE COUNT          337 a          425 c          506 g          524 t
ORIGIN      
        1 gaaacttggc attggcggct gaagcgggag ccacagttct cccggctatg ccggcctttt
       61 atcacaatgt gttaacggtc cgtgacctag tggacttcat cgtagcccgt attatcgatc
      121 aactcggtat tgaaaacgaa ttgatccgtc gttggggcac ataactcaga gtttttgcgt
      181 atgagagata atgcgtcttt ctcaaaagac gttgcgatga ccaatttggg aggcgaccag
      241 ttgggcgaag gtctcggatg taatttagga acagcgtttt cgccaatgac ttggttgagg
      301 aaggtgcgtc attttcttga gttgatcaga ttcagccaca ctctatttgc cctgcccttt
      361 gcagccttgt cagcggcgat ggtgtggaag gtagtgcctt ttcaatggca tcacctctta
      421 ggttttttac tttgtatggt ctttgctcgt agtaccgcaa tggcgttcaa tcgcctggtc
      481 gatcgaaagt tggatgcagc caatccccgt acggcgggcc gacatattcc gcgcggagtg
      541 ctgggagttc gtgaggtttg gggtttcact gccgtctgtg ccattgggtt tgtcttatcg
      601 acgttgctct tcttaccgaa ttattggccg ttggtagctt ctggtcctgt gctggctttt
      661 ctttgtggtt acagctacag caaacggttt acgtggttat cgcatttctg gctggggacg
      721 gcgttggctt tggcaccgcc gggtatctgg ttggccatcc aaggtacttt agcttggccg
      781 ccgatcctcc tcgcttgtgc cgtgatcttt tgggtggctg ggttcgatat tatctacgcc
      841 tgccaagact acgactttga tcgtcgccag aaactgtgga gcattcccgc tgtctttgga
      901 ataaggcgag ccctatggtt ggcggcggct tctcatggtg tgatggtggg atgcttggca
      961 atgctgccta ttctgtttaa cgaatttgat tggctttact gggtgggact tgcattggtt
     1021 tctgtgcttt tgatctacga gcattggctc gtccggccgg acgatctgcg acgagttaac
     1081 gaggcatttt ttcatgtcaa tgcggtggtg agcgttggtt tgttgatcat aggaactgtt
     1141 gacatttggc ttctgtaaaa acctctggga atcataatcc tcatgctaga ccagaacatc
     1201 gtgcgaacag agcgattagt tgaatcgcgt ctcgacgaga ttcgcaataa gatcgaggcg
     1261 gggcgtcgtt tgtcgtttga ggacggggtc tttcttttcc agccctctgt gtccttgcac
     1321 gttgtcggac agttggccga tcttgtttgc cgccgccggc atggtcgcac cgtctattac
     1381 aacttaaaca cccacctcaa cccgacaaat atctgcggtt accgatgcaa gttatgtgcc
     1441 ttctttaggt caaaaggtga ccctgatgcc tacatcatgt cgcaggaaga gattctaaaa
     1501 cgggcggagg atgccgatca ggcaggctgt accgagttgc atattgttgg tgggctaccc
     1561 ccgggcatcc cattcgagtg gtaccttgga attgttgagg cgatccataa ccagtacccg
     1621 cgacttcatt tgaaggcctg gaccgccgtt gagattgcct ggtttgcccg aatcagcggt
     1681 ctttcggtga aggaagtcct tctccgtcta attgacgccg gcttgggcag tctgcctggt
     1741 ggtggggccg agatcttcca ccccgaggtc cgcaggcaga tttcctccca ta
//
LOCUS       DAOPTR010000881         1791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_51520_length_1791_cov_3.022465, whole genome shotgun sequence.
ACCESSION   DAOPTR010000881 DAOPTR010000000
VERSION     DAOPTR010000881.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51520_length_1791_cov_3.022465"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PKI05_14125"
     CDS             complement(<1..362)
                     /locus_tag="PKI05_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53386.1"
                     /translation="MHRVISPSKKGHLPHIGGRHDPPGHANAMPLSFFKPQSVRGASE
                     SEKMEKLRRRKAINGECKRFGQDGREKQNLCKIGLFSFLSRQILPVDKAEVIRVTFLH
                     RPLGKRSYWFLKDKVFTA"
     gene            357..866
                     /locus_tag="PKI05_14130"
     CDS             357..866
                     /locus_tag="PKI05_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53387.1"
                     /translation="MQIPATFLRGLFSGVVGQPTRPVERNDQTKPQSADDKTLSDSLM
                     TVLGQSGNPSLWEILKEYDVTHITPRQFSQMLQKLRESGSLTEQEYTELAQIRRDLAE
                     AGVDADEPVDLIDFYQKFIRKLQLMQALGTAESTDDLQTALQRAEKHLKWLEKISTLQ
                     TELGTDAWA"
     gene            complement(991..>1791)
                     /locus_tag="PKI05_14135"
     CDS             complement(991..>1791)
                     /locus_tag="PKI05_14135"
                     /inference="COORDINATES: protein motif:HMM:NF015883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HOA53388.1"
                     /translation="RRRGYTPAAIRDFCQRIGVSKYDGVVDISLLEHCIREDLNKHAP
                     RVMAVLRPLKLVIDNYPEGQVEYLEAVNNPEDPSMGTRQVPFSKVLYIEQDDFREVPP
                     PKYYRLAPGREVRLRWGYFVRCTHVVKDPQTGEVIEVHCTYDPETRGGNAPDGRKVKA
                     TIHWVSAMHCIEAEVRLYDYLFTKPDPDDVPEGLDWRANLNPNSLEIIPNAKLERSLA
                     GAPVGSRYQFERLGYFCVDPDSTPEKLVFNRTVTLKDTWAKIQQKMGM"
BASE COUNT          396 a          509 c          471 g          415 t
ORIGIN      
        1 aacgctgtga acaccttgtc cttcaaaaac caataagatc ttttccctaa aggccgatga
       61 agaaacgtca cccggattac ttcggctttg tcaaccggca aaatctgccg agaaagaaag
      121 ctgaataagc cgattttgca taaattctgc ttttctcttc cgtcttgccc aaaccgctta
      181 cattcgccat ttatagcttt gcgccggcgt agtttctcca tcttctcgct ttcgcttgcc
      241 ccgcgcactg attgcggttt gaaaaaagac agcggcatgg cgtttgcatg tcccggcgga
      301 tcatgtctgc cgccaatatg cggcaaatgt cctttttttg atggagagat gacgcgatgc
      361 aaatcccagc cacctttttg cgaggtttgt tttcgggtgt cgttggtcag cccacacgtc
      421 ccgtggagcg aaacgaccag accaaaccgc agtcggccga cgacaaaacc ctcagtgaca
      481 gcttgatgac ggtgctcggt cagtcgggca atccatcttt gtgggaaatc ctcaaggagt
      541 acgatgtaac ccacatcact ccccggcaat tttcgcagat gctccaaaaa ctgcgagaat
      601 ctggttccct caccgagcag gaatacaccg agttggcaca aatccggcgt gatctggcgg
      661 aggccggtgt cgatgcggac gaacctgtcg atctcatcga tttttaccag aagttcattc
      721 gcaaattgca gcttatgcag gctttaggga ctgccgaatc aacagacgat ctccaaaccg
      781 ctttgcaacg cgcggaaaag cacctgaaat ggttggagaa gatctcgacc cttcagactg
      841 agctgggaac cgatgcctgg gcgtaggcct tgagccccgg tgcacttggc tttgtggggg
      901 ccgcgcaaat caccgccatg aagcaagccg agagccccac catgtgcggc ggccgcttct
      961 ttctctgcgg cttgcctgac gcacctcgtg tcacattccc attttctgct ggattttggc
     1021 ccaggtgtcc ttgagggtga cagtccgatt gaagaccagt ttctctggcg tactgtccgg
     1081 atcgacacag aaatacccca atcgctcgaa ctgataccgt gaccccacag gggcccccgc
     1141 aaggctccgc tccagttttg cgttgggaat gatctctagc gagttgggat tcaaattggc
     1201 ccgccaatcg agaccttcgg gcacgtcatc gggatcgggc ttggtaaaga ggtaatcgta
     1261 cagacggact tccgcctcga tgcaatgcat ggcagaaacc caatgaatgg tggccttgac
     1321 ctttcggcca tctggggcat ttcccccgcg ggtctcggga tcgtaggtgc agtggacctc
     1381 gatcacctct cctgtttgtg ggtctttcac gacgtgcgtg cagcggacaa aatagcccca
     1441 ccggagtcgc acttcccgcc ccggcgctaa acgatagtac ttcggcgggg gaacctcgcg
     1501 gaagtcatct tgttcgatgt agaggacctt agaaaacggc acctgacgcg tacccatgga
     1561 aggatcctca gggttgttga ctgcctcgag atattcgact tggccttccg gatagttgtc
     1621 gatgacgagc ttcagaggtc gtaggacggc catcacccgc ggggcatgct tgttaagatc
     1681 ctcccgaatg caatgctcca gtaagctgat atcgacgacc ccgtcgtatt tcgaaacgcc
     1741 aatcctttgg caaaagtccc gaatcgctgc cggagtgtaa ccgcgacgcc g
//
LOCUS       DAOPTR010000882         1790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_51561_length_1790_cov_3.074928, whole genome shotgun sequence.
ACCESSION   DAOPTR010000882 DAOPTR010000000
VERSION     DAOPTR010000882.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51561_length_1790_cov_3.074928"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="PKI05_14140"
     CDS             complement(<1..892)
                     /locus_tag="PKI05_14140"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA53389.1"
                     /translation="MFGKRVPGYPHLVLLSVFFGSITASFVTTLAEERPNILFCLADD
                     WSYPHASAYGCPWIQTPGFDRVAKEGILFTRAYTPNAKCAPSRSIILTGRNSWQLKEA
                     CNHNCEFPLEFKVVTEVLEEHGYFVGKTGKGWGPGRARDPNGQPRPLLGKSFDKHTTT
                     PPTPTISRNDYAANFAEFLKEKPQDRPWFFWYGSTEPHRAYQFQSGLNLTGKKLSDVN
                     WIPPYWPDNDIVRTDLLDYAFEVEHFDRHLQKMLELLEQTGELENTVVIVTSDNGMPF
                     PRVKGQCYELSNHMPLAIMWK"
     gene            complement(1351..>1790)
                     /locus_tag="PKI05_14145"
     CDS             complement(1351..>1790)
                     /locus_tag="PKI05_14145"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HOA53390.1"
                     /translation="RERSMADVELLMESDVTKENLAALFRRAFFKTTFDSDGDLVVET
                     DGPRVLVTIDEERRLLKYMAIYGVLEWSPLEEKLELSNALNDKFIFARFSIPEKRDDV
                     LIADYFLPYEGGIPAFQIIAAVRLFSRVVPAAIRVCDENNIVE"
BASE COUNT          433 a          501 c          446 g          410 t
ORIGIN      
        1 ttttccacat gatcgccaag ggcatatgat tggaaagctc gtaacactgt cctttgacgc
       61 gtggaaacgg catcccgtta tctgacgtga caatgaccac cgtgttttcc agttcgcccg
      121 tttgttctaa aagctccagc atcttttgga ggtgccgatc gaagtgttcc acctcaaaag
      181 cgtaatcgag aagatcggtc cgcactatgt cgttgtccgg ccagtacggc gggatccagt
      241 tgacatccga gagcttctta cctgttaagt tcagcccact ttggaattgg taggcccgat
      301 gaggctcggt tgacccatac cagaagaacc acggccgatc ttgcggtttc tccttcagga
      361 actctgcaaa gttcgccgcg tagtcattcc gcgaaatggt cggcgtcgga ggtgtggtcg
      421 tatgcttgtc aaaagactta cccaaaagtg gacgcggctg tccatttggg tccctagctc
      481 gccccggccc ccacccctta ccggtctttc ctacaaagta accgtgttcc tctaagacct
      541 cggtaacgac cttgaactcc agcggaaact cgcaattgtg gttacaggcc tccttgagtt
      601 gccaggaatt gcgaccggta aggatgatag accgactcgg cgcacatttg gcattggggg
      661 tataagcccg ggtgaagagg attccctcct ttgcgacgcg atcgaatcca ggggtttgaa
      721 tccatgggca gccatatgca ctggcgtgcg gatatgacca gtcatcggcc aagcagaaaa
      781 ggatattagg gcgctcttcc gccaacgtcg tgacaaagga cgcagtgatc gacccgaaaa
      841 acaccgacaa caatactaga tggggatatc ctggaactct ttttccgaac atgggcgtgc
      901 ctccccgtcg aaaaccgtgg tttcaccaaa agcagccgtc acagcgatat tgtaaaaagt
      961 gccggcgaat cctcaaggta tgctttcatc gatccgtgcc cttatcaaca gtgctcttta
     1021 ggacgtagct tccgctcgtg tgaaacgcct ttggccgcgg acgaaaatcg aaaccagatg
     1081 gggtagctcc aaagtgtacc cgactggatg agaggcccaa tggcacagta gagttggcta
     1141 tggactcgcc ctggtgtccg atgtgatacc aaccggccct ctaatcactt gacacaacca
     1201 cgactgcgaa tggcccaaat caggtcacat ctaacacaga aaacctggag cagggtgccc
     1261 cgttacatcc cgtgtactcc cgaactgcat atcaaccatt tgacgcggct ttgatggcgc
     1321 gtgacccttc gcaggcaaca attccgtcga ctattccacg atattgttct catcgcagac
     1381 acgaatggca gcgggtacga cacgtgagaa caaacggacg gctgcaataa tctgaaacgc
     1441 cggtatcccg ccctcgtagg gaagaaaata gtccgcaatc agaacatcat cacgtttttc
     1501 ggggatcgaa aagcgggcaa aaatgaactt gtcgttaagg gcattgctca gttctagctt
     1561 ctcctcaaga ggtgaccatt ccaggacccc gtagattgcc atgtacttga gaaggcgtct
     1621 ttcttcatcg atcgtcacca ggacacgagg gccatccgtc tctacgacaa ggtcgccatc
     1681 gctatcgaat gtggtcttga agaacgcccg tctgaagagc gccgccaggt tctcctttgt
     1741 cacatcactt tccatcaaca gttcaacgtc cgccatactc ctctcccttc
//
LOCUS       DAOPTR010000883         1789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_51597_length_1789_cov_2.833910, whole genome shotgun sequence.
ACCESSION   DAOPTR010000883 DAOPTR010000000
VERSION     DAOPTR010000883.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51597_length_1789_cov_2.833910"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            586..>1789
                     /locus_tag="PKI05_14150"
     CDS             586..>1789
                     /locus_tag="PKI05_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53391.1"
                     /translation="MAQPLSQISHWCENTNWLKRLAQYSIVCLLVVSLGCSRGEDTRK
                     AEQQKASPQDTSHTSEEVEIPLELWYVNYVLGERVGYERQSFDFADEDGRRLRKVSGE
                     SCLRVKRASTIIEMRIRHTCWETLEGWPVRFEVETDQGESTHRTRGEIRDGQLIVETE
                     VAGQAVQKSLPCPANCRGFLGLQESLFEKPMSPREERDLTTLVPGLNDIAYMTLKAND
                     YTSLQVGNRDVRTLSIQFTMTLNSGGIFRGQLWVDERGIIVKQETDSPPMTTILATRG
                     DALEQSPDPRLDLVRDIRIPVKGAPFPVERAAQAVYKISCDTEDLSQLLPSDCHQTVK
                     RLGPHTVELDVHPLSLSPEMPDCHESADPSTTQATLWVQSNAPEIIRMAEMAASGISD
                     PQDVAPALA"
BASE COUNT          414 a          506 c          466 g          403 t
ORIGIN      
        1 cgcgtagttc acgtcatgag aacccttttt gatcctgacc gttccatatt ggggtgggcg
       61 accaatcacg ccgtcccctg ctgtaaccta ggccatatct agggctccat cacgtctcgt
      121 tctctccatt ttttcgcttg cgattttggt tatcccgcaa aacccgtacg atcaactaaa
      181 tcccgccaca ctttccgcga cacgctttcg tctcggggaa gagactgata tgggcacaag
      241 tcgtgaccag gctactggaa aagccgtttc ccgtcccgca tcgcgtctcc taaaagaact
      301 ctagcaccaa attcggcccg atgtttcttc aaagacaatg gcccacagcc tcatgacgac
      361 tcatttgcaa cgaacacaca gaccgcgcaa aaactcgcgg ttacccaaga acctccggcc
      421 gtgctgcttc ttgcgaccag acaagatgat gcagacaaag aagatcacag gtcggtgtca
      481 cccttgacct atgtcgagag gccttcgata tactgaagct gatgcccgac cgaaacccgc
      541 acagtggctt tggcgtgagg tcttttagcg aacataaaac gacaaatggc ccaacccctt
      601 tcgcagatta gtcattggtg tgagaatacc aactggctca aacggttggc tcaatatagt
      661 attgtctgtc ttctcgtggt ctcgctgggg tgttcaagag gtgaggacac gagaaaggct
      721 gagcagcaga aggcctctcc gcaggacacc tcgcatactt cggaagaagt ggaaattccc
      781 ctggagcttt ggtatgtcaa ctatgtgctg ggagaacggg tcggttatga acgacagtcc
      841 tttgattttg cggacgaaga cggtcgccgc ctccgcaagg tctccggaga gagctgcctg
      901 cgtgtcaaac gcgcgagcac gattatcgag atgaggattc gtcacacatg ctgggagacg
      961 ttggagggct ggcccgttcg ttttgaagtg gagacggacc agggggaatc cacccatcga
     1021 acaagagggg aaatccgcga tgggcagctt atcgtggaaa cggaggtggc aggtcaagcc
     1081 gtccagaaaa gcctcccctg tcccgcgaac tgccgtggtt ttctaggact tcaggaatcg
     1141 ttgttcgaaa aaccgatgtc tccccgcgaa gagcgtgatc ttacgaccct tgtgcccgga
     1201 ctgaatgaca ttgcctatat gacattaaag gccaacgatt acacttcact gcaagttggc
     1261 aatcgcgacg tccgtacctt atccattcaa ttcaccatga cactgaattc cggcggtatc
     1321 tttcggggac agttgtgggt cgatgagcgg ggtattattg tcaagcagga gaccgattca
     1381 cccccgatga caacgatcct tgcaactcgg ggagacgcct tggagcaatc ccctgatccc
     1441 aggcttgatc tcgttcggga tattcgcatc cctgtcaaag gagcaccatt tccggtggag
     1501 cgtgccgcgc aagccgttta taagatctcc tgcgatacgg aagatctgtc ccagttgctt
     1561 ccctccgact gccatcagac ggtgaagcga ctcggtcctc ataccgtgga actggacgtt
     1621 catccgttgt cgctgtcgcc tgagatgccc gactgccacg aatcggccga tccttccacc
     1681 acccaggcca ccttgtgggt ccaatcaaat gctcctgaga tcatccgcat ggctgaaatg
     1741 gcggcctcgg gaatctcaga cccgcaagac gttgccccgg ctttggcca
//
LOCUS       DAOPTR010000884         1789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_51598_length_1789_cov_2.819493, whole genome shotgun sequence.
ACCESSION   DAOPTR010000884 DAOPTR010000000
VERSION     DAOPTR010000884.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51598_length_1789_cov_2.819493"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="PKI05_14155"
     CDS             complement(<1..164)
                     /locus_tag="PKI05_14155"
                     /inference="COORDINATES: protein motif:HMM:NF014611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOA53392.1"
                     /translation="MPLVLRSVKEMRAYSRSAKQSGSRIGFVPTMGALHRGHLSLVEA
                     AMKECETVVVS"
     gene            713..1471
                     /locus_tag="PKI05_14160"
     CDS             713..1471
                     /locus_tag="PKI05_14160"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HOA53393.1"
                     /translation="MTTLSKNYFFGIGGKVVGPLDEKEIGRLIAEGIVNANTLAWCVG
                     QARWEPVGAIEELQQAFGPLLEGQQVGAATAATSPDVPPPLPEDQTVASTPLSHKAKV
                     QANTGHLSPLQSAAYSMVCWLYRPWRGRRSIIREFVDQDPTRAVHVAIGTMVVLGILF
                     VMTIGAMFPPEEPSRSLPSQPTAGANTLGANTVGAMPPGGQLSPNVFLPMIRAQQDIN
                     NTIDDVYRYNRDSFDRQCETYRRGTYDWYSSSND"
BASE COUNT          415 a          505 c          496 g          373 t
ORIGIN      
        1 gatacaacga cggtctcgca ctccttcatg gcggcctcca ccaaactgag atgtcctcgg
       61 tggagggccc ccatggtggg gacaaacccg atacggcttc cggactgctt ggcggaccgc
      121 gagtaagccc gcatctcctt caccgaacgc aaaacaagcg gcatggccat ctttagaccc
      181 ctattcgata tcgcaagagg aatcaccggc cttcatgcac ggtctcttgt catcgcggcg
      241 acaagagcta ttgctggacc gtaaaacatc tactttacca cagatggcag tttgccaagc
      301 aggtcccagc ggttcgctgc gttgctcccg acaaagccgg actgatttcg cggacgccaa
      361 cggccacacg caaaccaaaa atgggcagct atttcttgac attattaccc cctcagccga
      421 ggggcatttc gtgacctaag tttttattag ggccggtact gtgtgaccgg tgcagtcttg
      481 cgcggatgag tgagacctcg aggtaatcgg ccttgtgtca tcacggacca gccttctgca
      541 ccaaatggcg cactgagcct cgtttcacaa tcgtcgatca cctggttgga aaaagtctcg
      601 ctgtcatctt ccaaaggaag cgcgcgggct cgctgagatc atccgatagt ggcccaccgg
      661 ttgtagggct tggcaaaaca ccaaataaat cctcagtagc ggagactcat tcatgacgac
      721 actgagtaag aactacttct tcggcatagg aggcaaggtt gtcggaccgc ttgacgaaaa
      781 ggagatcgga aggctgattg ccgagggcat tgtgaacgcc aatacgctcg cttggtgtgt
      841 ggggcaggca cgatgggagc ctgttggggc gatcgaagaa ctgcagcagg cttttggtcc
      901 tctcttggaa ggtcaacagg ttggggcggc aaccgcagcc acatccccgg atgttccgcc
      961 accgcttccc gaggatcaaa cagtagcttc aactccattg agtcataaag cgaaggttca
     1021 agcgaatacc ggccacctca gtccattaca gtcggcagcc tacagcatgg tgtgttggct
     1081 ctatcgcccg tggcgaggac gccgttccat aatccgagag ttcgtggacc aggacccgac
     1141 acgtgccgtg cacgtggcga tcgggacgat ggttgtcctt ggaatactct ttgtgatgac
     1201 aatcggggcg atgtttccac cggaagagcc atcgcgttcc ctgccttcac agcctacagc
     1261 gggcgcaaat acactaggcg caaataccgt gggcgcaatg ccgccaggtg ggcaattaag
     1321 ccccaacgtg tttctgccga tgatccgtgc tcagcaggat atcaacaaca cgattgacga
     1381 cgtctatcgc tataaccgtg actcgttcga tcgccagtgc gaaacctaca ggaggggtac
     1441 ctacgattgg tacagctcga gcaatgacta agagcctatc ccaaaaagtc cccctctccc
     1501 tctgggagag ggttggggtg aggggaagcg accgtcggag tccatcgaga aaaccggcag
     1561 cgattcgagc taccctcgcc cccgacccct ctcccgccga acgcgcgagg ggagattatg
     1621 ggataggctc tcaagtagcg caaattgtgc tattgagaaa tggcttcggg gcgaggaagc
     1681 cccacctctg tcaaaagcca tggcgttctt tttgaatccc tcaagttgcc gggatattcc
     1741 agaagacggc tcggtccctt tttgagactt ggctgaaccg ataaaagcc
//
LOCUS       DAOPTR010000885         1789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_51605_length_1789_cov_2.316609, whole genome shotgun sequence.
ACCESSION   DAOPTR010000885 DAOPTR010000000
VERSION     DAOPTR010000885.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51605_length_1789_cov_2.316609"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            532..1530
                     /locus_tag="PKI05_14165"
     CDS             532..1530
                     /locus_tag="PKI05_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53394.1"
                     /translation="MWDRPQRFRRRPDRHLFACSLQVRAVVLSATALVMALASRGYPP
                     VFAQQVRLDVTNSSVSEGFFEYQGTSWTLMGPSGFARFGQSHTQVSPFPVFESPPPAL
                     GVHYGSRGWQGEFFGYWSSGIQRSHRSLTLSQTLSDGIYGSFACQSLRPFVIGFHPVF
                     ASAPYAPFAEPPALAYQHLPNPGPPMPNPRPAVAKDTQRAAARAPADRENDLPVEKLE
                     GSARSEDEPPLVLGGRPGPAEAADSAKGDRIRSLSQSSAERVVMSVDEARALRSAEQQ
                     RDNQEAQRYCDLAEAAMKRGEQGQARIYLRLAEKLASGDLRRKVLLLQQNLGEAEE"
     gene            1559..>1789
                     /locus_tag="PKI05_14170"
     CDS             1559..>1789
                     /locus_tag="PKI05_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53395.1"
                     /translation="MDFVYSGQLAARTIEGPKVAAAYPFSLRRRRDNTSTMAEQTSES
                     ERHLISRVRSGDPEAWEEVIRRYEGRLYSYLGS"
BASE COUNT          374 a          512 c          501 g          402 t
ORIGIN      
        1 accttctcca acacttatcg tgccggacaa ggtgttgcga acacatgaaa ggcttgatca
       61 caggaccact cccggtcgcc cggagtggtt ttttaagcat ccggtttacc agatgcgttt
      121 aggggcttgt aaccgcacgc taccaaattg tcaaagagca acgcttaaac tttgtgccac
      181 cgtaccccgg cggcaatttg aattatagca cccgctttca tgcggtcaac aggggggtat
      241 tccgcctcct gccgaccctt ttttccaccc catcttacgg attaagttac cgtcttaagg
      301 tgggctttac taggcggggc agccttttca ggcatccttc ggccattatt gcctcggaca
      361 ctttttatgt tatcgtccga ttcttgcccc gactagaggt cctctcgcaa aatcttttga
      421 ccgctcggca gggctcagac aatagccttt cgcatgcctg acaattacaa taatgagcga
      481 cggaccaaaa ctcgtcaagg ggtacgcctg gtcaaaagag ggtcacggct tatgtgggat
      541 agacctcaaa gatttcggcg tcggcccgat cgacatcttt tcgcttgttc attgcaagtg
      601 cgggcggtcg tgctgtccgc cactgcactt gtcatggccc ttgcgtctag aggatacccc
      661 ccagtattcg cccaacaggt gcgtctcgac gttaccaact cttcggtctc cgaggggttc
      721 tttgaatatc aggggacaag ttggaccttg atgggaccga gtggctttgc ccggttcgga
      781 cagtcgcaca cgcaggtttc gcccttcccc gtgttcgaga gtcctccgcc ggccctcggg
      841 gttcattacg gaagtagagg gtggcaaggg gagttctttg gttattggtc atccggcatc
      901 cagcgaagcc accgttcact gacgttgtcg caaacgcttt cggacggtat ttacggctcc
      961 ttcgcatgcc aatcgttaag accgttcgtt atcggcttcc atccggtctt cgcatcagct
     1021 ccttacgctc cttttgccga acctcccgcc ctagcgtacc aacacttgcc caaccctggg
     1081 ccgcccatgc cgaacccaag accagccgtg gccaaagata ctcagcgcgc ggcggcgcga
     1141 gcaccagcag accgtgagaa cgatcttcct gtggaaaagc tggaagggtc agctcgctcg
     1201 gaagacgaac cgccgttggt actcggtggc cggccgggtc cggccgaagc cgcggattcg
     1261 gccaagggcg ataggattag atcgctatct cagagttccg ccgaacgggt cgtgatgagt
     1321 gtcgatgaag cacgggcgct tcggtccgct gagcaacagc gggacaatca agaagcacaa
     1381 cgttactgcg atttggcgga agcggccatg aagcgcggtg agcaaggcca agcgagaatc
     1441 tacttacggc tggcggaaaa gcttgcctcg ggagacctcc gcaggaaagt cttactgctc
     1501 cagcagaacc ttggcgaggc cgaggagtga tcggccccgc tccagggaag acttgccagt
     1561 ggatttcgtg tattctgggc agttagcagc caggacgatc gaagggccaa aggttgccgc
     1621 cgcgtatccg ttttccctcc gcaggcgacg agataacaca agtaccatgg cggaacagac
     1681 atcggagagt gaacgacatc tcatttctcg tgtccggtcg ggtgatcccg aggcatggga
     1741 agaggttatc cgccgctatg agggccgact ttactcatac cttggtagt
//
LOCUS       DAOPTR010000886         1787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_51667_length_1787_cov_4.412240, whole genome shotgun sequence.
ACCESSION   DAOPTR010000886 DAOPTR010000000
VERSION     DAOPTR010000886.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51667_length_1787_cov_4.412240"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PKI05_14175"
     CDS             <1..419
                     /locus_tag="PKI05_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53396.1"
                     /translation="DLISVSSSPASDRRYQLFDGDVIYVPERKVDPIFVDGLVTKPGM
                     YEWPNNQEVRLLDALAMAGGRNNPLADNVWILRHVPGREEPIVIKAKLNAIKRNAAEN
                     IRIAPGDVITVEETPGTFLYSLLKGFVHLGGSIPLN"
     gene            489..>1787
                     /locus_tag="PKI05_14180"
     CDS             489..>1787
                     /locus_tag="PKI05_14180"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOA53397.1"
                     /translation="MNDFSLASSAVPESTTHLLQVAVQFISAVRRRWHIVATSVLATG
                     ILGALYYATATRYYTSKAEVLILQAGDNPLSPTIQSQNNRENNLMPTFVNLMTSQKVL
                     EGAIRQLSPEDCLVDLNPDKPERWPAQLRAGLTVTSVGRSNIIAVEYKSRHPEVAARV
                     VNAVVQSYLDFMRTTTQGTAAEIVHVLNTEKDQIAAQLSEKEQALQQLSQQIGALDTG
                     VNSQAIHPLVERARAFNNALVEAQKTRVNLEATIQAVEAALRNGQDIREYLFSGSDTV
                     GQQLLLGLLGFDREESIARANMERQLLEDRVQLANVQKYLGNNHPQVQLLQQRIAATE
                     DYLRNYQRRIEARLAMLGDSELTSLLLGTLRHRLQESVQKEHALQLQYEAARQQAATL
                     AGGLAQMEMLKNDIAGLRELYSGLVRRITNMDLTGDLRAAL"
BASE COUNT          435 a          498 c          484 g          370 t
ORIGIN      
        1 tcgatctcat ctcggtgagc agttcaccgg cgtcggatcg tcgctatcag ttgtttgatg
       61 gggacgtgat ttatgtgccg gagcggaaag tggaccccat ttttgttgat ggtcttgtga
      121 caaagcccgg tatgtacgaa tggcccaata atcaggaagt tcgcttacta gacgctctgg
      181 ccatggccgg gggaaggaac aatcctctgg ccgacaatgt gtggattctt cgccatgtgc
      241 cgggtcgcga ggaacctatt gtgatcaagg ccaagctcaa tgccatcaaa agaaacgccg
      301 cggaaaacat ccggatagcc ccgggcgacg tcattacggt tgaggaaact ccgggaacgt
      361 tcctttacag tctgctgaag ggattcgtgc atttgggcgg ttccatcccg ctgaattgat
      421 atgatgggcc cagaggccaa gtcctaacca acgttcgaca aatcagtcat gacaagacga
      481 tctatggcat gaacgatttt agcctcgcgt cttctgcagt ccccgagtca acgacccatc
      541 tccttcaggt cgccgtccaa tttatcagtg cggtacggcg gcgttggcac attgtagcca
      601 cctcggtctt ggcgacgggc attctcggag cactctatta cgccacagcc acgcgatatt
      661 acacttccaa ggccgaggtg ttgatccttc aagctggcga caaccccctt tcgccgacga
      721 ttcagtctca gaacaaccgt gaaaacaacc tgatgcccac cttcgtcaac ctcatgacga
      781 gccagaaggt gctcgaaggg gccatccgcc aactttcgcc ggaagattgc cttgtcgatc
      841 tcaacccgga taagccggaa cggtggcctg cccaactccg tgccggattg acggtaacct
      901 ccgtgggacg aagcaacatc atcgcggttg agtacaaatc gcgtcatccg gaggtcgcgg
      961 cgcgggtcgt caacgccgtt gttcagtcgt atttggactt tatgaggacc accactcagg
     1021 gcaccgcagc ggaaattgtg catgtcctca atacggaaaa ggatcagatc gccgcccaat
     1081 tgagcgaaaa agagcaggcc ttacagcaat tgagtcaaca gataggggcc ttagacacgg
     1141 gggtcaactc gcaagcaatt catcctttgg tagaaagggc gagggccttc aacaatgcac
     1201 tggtcgaggc acagaagact cgagtaaacc tcgaggccac catccaggcg gttgaagccg
     1261 ccctccgcaa tgggcaggac atccgcgagt atttgttcag tggttcggac acggtcggcc
     1321 agcaactctt gcttggcctt ctcggtttcg accgagagga aagtattgcc cgggcaaaca
     1381 tggaacgtca actgctcgaa gaccgggtgc agttagccaa cgtccaaaaa tatttgggga
     1441 ataatcaccc ccaagtgcaa ctactgcaac agcgcattgc cgcaaccgag gattatcttc
     1501 gcaactatca gcggcgaatc gaagcacgct tggcaatgct cggagactcc gagttgacaa
     1561 gccttctatt agggacactc cgccaccgcc tccaagagag tgtgcaaaaa gaacacgctc
     1621 tgcaacttca gtacgaggcc gcccgccagc aagccgcaac tttagccggc ggacttgccc
     1681 agatggaaat gctcaagaac gacattgcgg gcctgcgaga gctttacagt gggctagtcc
     1741 ggcgtatcac caatatggat ttgacaggcg atctgcgagc agccctc
//
LOCUS       DAOPTR010000887         1787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_51677_length_1787_cov_3.062356, whole genome shotgun sequence.
ACCESSION   DAOPTR010000887 DAOPTR010000000
VERSION     DAOPTR010000887.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51677_length_1787_cov_3.062356"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1445)
                     /locus_tag="PKI05_14185"
     CDS             complement(<1..1445)
                     /locus_tag="PKI05_14185"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOA53398.1"
                     /translation="MTWKKIVLNFSRGAVILPVLGGIKLPRATVGHREFRLCRSWSFV
                     VVVAVLVLWACRGLDAGEVLFRWDFEPQGVDSVGERISTMPDGFQPGQGDGLEIAKTA
                     DRGWALHVSIPAGAPGHRNIRYMIPADRVRGKRLKVSANIKAQGISQPPNPWNGIKCM
                     LQIVSPGETQWPQQNMPGGDFDWRRASFLAVIPDDVERVFLILGLENVSGDAWFDDVV
                     IEDLGARKVPAAATSQPVFMGHQLPRLRGAMIGPRVTDEDLLEFGQVWKANHVRWQLI
                     WGGFPRSPADGASLKEYRDWLETALRRLDEALPVCRKAGILVTVDLHTPPGGRDSSSA
                     CRMFQVREFQEAFIQIWEEIARRYKDSNVVWGYDLVNEPVEGAIPAGLLNWQELATET
                     ARRVRAIDDHHAIIIEPAPWGSPSSFDLLEPLDVPGVVYSVHMYIPHSFTHQGVYNNP
                     MGIVYPGMIDGRWYDRETLKKALSPVVKFQR"
     gene            complement(1520..>1787)
                     /locus_tag="PKI05_14190"
     CDS             complement(1520..>1787)
                     /locus_tag="PKI05_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53399.1"
                     /translation="ALWPEGAESLGYGRGLIAGEALYWPTLDAIYIIDALSAQPKKIF
                     PLGPWHCEGGNLLWADGYLLVASSTELAVFQTSRPHVKTEIRSP"
BASE COUNT          425 a          561 c          418 g          383 t
ORIGIN      
        1 tcccgctgaa acttcacaac tgggctaagt gcctttttga gtgtttctcg gtcgtaccag
       61 cggccatcga tcattcccgg ataaacgatc cccatggggt tgttgtacac accctgatgc
      121 gtgaagctat gtggaatgta catatggaca ctataaacca cgcctggaac atcgagcggc
      181 tccagcaaat caaacgatga cggacttccc catggcgccg gctcaatgat aatggcgtgg
      241 tgatcgtcga tggctcgaac acgccgggcc gtttcggtag ccagttcctg ccaattgagc
      301 aggcccgccg ggatggcccc ttcgaccggc tcattgacca aatcgtatcc ccataccacg
      361 ttgctatcct tgtaacgtcg tgcgatttcc tcccaaatct ggatgaacgc ctcctgaaac
      421 tcccgtacct gaaacatccg gcacgcggag gaactatccc tgccaccggg cggcgtatgc
      481 agatccacgg ttaccaaaat cccagccttg cggcagacag gcaacgcctc atccaaccgg
      541 cgaagcgccg tctctagcca gtcacggtac tccttcaaac tggcgccatc ggcagggctt
      601 cgtgggaagc caccccaaat cagttgccaa cgcacgtgat tggccttcca aacctgtcca
      661 aattcaagaa ggtcctcatc tgtaacacgc ggtccaatca ttgcaccgcg taatcgcggc
      721 aactgatgtc ccatgaagac aggctgactt gtcgcggctg ccggtacctt tcgggccccc
      781 aagtcctcga tgactacatc atcgaaccag gcgtcgcccg atacgttctc aagacctaag
      841 atcagaaaaa cccgctccac atcatctgga atgactgcca aaaaactggc acgccgccaa
      901 tcgaagtcac ctcccggcat gttttgttgg ggccattgcg tttcgcccgg cgaaacgatt
      961 tgaagcatgc acttgatgcc gttccacgga ttgggcggtt ggctaatacc ttgtgccttg
     1021 atatttgcag aaactttcaa ccgctttcca cgaactcgat ccgccgggat catatatcgg
     1081 atattccgat gccccggcgc tccagcgggg atcgaaacat gtaacgccca cccccggtcg
     1141 gccgtctttg caatctccaa gccgtctcct tgccctggtt gaaaaccatc gggcatggtc
     1201 gatatccgct cgccaacact gtccaccccc tggggctcaa agtcccatcg aaacaaaact
     1261 tccccagcgt ccagcccacg gcaagcccat aaaaccagaa ctgccacaac aaccacgaaa
     1321 gaccaggagc gacataatcg gaattctcga tgcccaacag tcgcgcgagg taatttgatc
     1381 cctcccagaa cggggagaat tactgcaccg cgcgaaaagt ttaggacgat tttcttccaa
     1441 gtcataagat tgtccttgcc ttgatacgag cgttttcttt tcaacccaca cccctgttcc
     1501 atcatcttaa caaacaacct tacggggaac gaatctcggt cttgacatga ggtctgcttg
     1561 tttgaaatac agctagctca gtggatgatg ccactagcaa atacccgtcc gcccaaagga
     1621 ggttcccccc ttcacagtgc cacgggccta gcgggaagat ttttttcggc tgggcactga
     1681 gagcgtctat gatgtaaatt gcgtccagtg ttggccagta caaagcctct cccgcaataa
     1741 gtccccgacc ataacccagg gattccgcgc cctccggcca aagcgcg
//
LOCUS       DAOPTR010000888         1786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_51707_length_1786_cov_3.943963, whole genome shotgun sequence.
ACCESSION   DAOPTR010000888 DAOPTR010000000
VERSION     DAOPTR010000888.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51707_length_1786_cov_3.943963"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PKI05_14195"
     CDS             <1..185
                     /locus_tag="PKI05_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53400.1"
                     /translation="AGRGRATLRRGWVNPRVREGKIRRTLGSHVSLGKTPGTASLPSS
                     EVRRVIFHKADGGRIN"
     gene            complement(315..1667)
                     /locus_tag="PKI05_14200"
     CDS             complement(315..1667)
                     /locus_tag="PKI05_14200"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOA53401.1"
                     /translation="MRVLIVDDEKIKRVTLADDLAAQGYEVETAADGEEALERLRDRH
                     FDVVVADVKMPRVDGLELLRYIKQGEQAPATDVIMMTAYGSIQLAVESMKLGAYDFIT
                     KPFRNEDLFPVLARLQKERGRRISSREVEGPKANDIESSVIGQSAVMTRVKKMIEICT
                     RTDANVLLCGETGVGKDLIAQVIHRNSHRAQQPFVKVGCTLFPAHLIESELYGHEKGA
                     FTGADQRRPGRFEIAQGGTLYLDDVDDIPLEQQAKLLRVIEEKVFERVGGTTPIQADV
                     RIIASTKQNLLEKTAQGTFRQDLYYRLDVLRINIPPLRERREDIPLLAEHFMKKIAGD
                     QPYKIDPAAIQVLQEHEWPGNIRELYHTLERAYLMGSGNITADVVRAEMAYFPIKDMK
                     GTAAKLMPSTFQDAINQAERELLEKALREAGGNKTAAAAALGMKPSTFRDKLAKHGLG
                     "
BASE COUNT          352 a          509 c          503 g          422 t
ORIGIN      
        1 tggccgggag agggagggcc acgcttcgtc gtggttgggt caacccgcgc gtccgcgaag
       61 gaaaaatacg gcggacgctc gggtcgcatg tctccctggg taagacgccg gggacggcgt
      121 ccctaccatc gtcggaggtg cgaagagtga tcttccacaa ggctgatggt ggaaggatca
      181 actgacgcgg aggtttgata ttcggcctcg ggcagggatt ttcagaggaa agccagacac
      241 cccgcggact ggcggggtgt cttttttcat cgccgtcctg tcgcgaccac gggtcgtgct
      301 gcgaagacga tctttcagcc gagaccgtgt ttggcaagtt tgtcgcggaa ggtagagggc
      361 ttcatcccca gtgctgccgc agccgccgtc ttattgccac cggcctcgcg aagtgccttt
      421 tccaacaatt cccgttcggc ctgattgatg gcatcctgga acgtggaagg cattagtttc
      481 gctgctgtgc ctttcatgtc cttgatcggg aaataggcca tttcggcacg gacgacatcg
      541 gcggtgatgt taccgcttcc catcaaatag gctcgttcca gggtgtgata gagctcgcga
      601 atattccccg gccactcgtg ctcttggagg acctggatag cggccggatc gattttataa
      661 ggctggtcac ccgcgatctt tttcatgaaa tgctcggcca gcagaggaat gtcctcacgt
      721 cgttcgcgaa gcggtgggat attgatgcgg aggacatcaa ggcgatagta gaggtcctgc
      781 cgaaacgtcc cctgggctgt cttttccagg agattctgtt ttgtcgaggc gatgatccgc
      841 acatcggctt ggattggcgt cgtccctccg acccgctcaa aaactttctc ctcgatgacg
      901 cgcaggagtt tggcctgctg ttcaagggga atatcgtcca catcgtccag atagagcgtt
      961 ccaccctggg caatttcgaa tcgcccagga cgccgctgat ccgcacctgt aaaggcccct
     1021 ttctcgtgcc cgtagagttc gctttcgatg aggtgggccg gaaagagtgt acatcccacc
     1081 ttgacgaacg gttgttgagc cctgtgagaa ttgcgatgaa tgacctgggc aatcaaatcc
     1141 ttgccgacgc cggtctctcc acacaggaga acattggcgt ctgtccgcgt gcagatttcg
     1201 atcattttct tgacgcgggt catgacggcc gattgcccaa tgacggagct ttcaatgtcg
     1261 ttggcctttg gcccctcaac ttcgcgggaa ctgattcgcc gtccgcgctc tttttgaaga
     1321 cgggcaagca ccggaaacag gtcttcgttg cgaaacggct ttgtgataaa gtcgtaagcc
     1381 cccaatttca tcgactcaac ggccaattga atgctcccgt aggcggtcat cataatcacg
     1441 tcagtggcag gggcctgctc gccttgtttg atgtagcgga gcagctccaa accgtccacc
     1501 cgcggcattt tcacatccgc gaccacgacg tcaaaatgtc ggtcgcggag cctttccaga
     1561 gcctcctcac cgtcggcggc agtttctact tcgtaaccct gggcggcaag atcgtccgcc
     1621 aaggtcaccc gtttgatttt ctcatcatca acgattaaca ctcgcataga gtttatccga
     1681 tcttatcgac gatctccgtg tgggtcgcga taagcgttca agataaccgt aaacgttgtc
     1741 ccctgcccca aggcactccg aacctcgata cgtcccccca tttccg
//
LOCUS       DAOPTR010000889         1786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_51718_length_1786_cov_3.046216, whole genome shotgun sequence.
ACCESSION   DAOPTR010000889 DAOPTR010000000
VERSION     DAOPTR010000889.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51718_length_1786_cov_3.046216"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            269..1132
                     /gene="trxA"
                     /locus_tag="PKI05_14205"
     CDS             269..1132
                     /gene="trxA"
                     /locus_tag="PKI05_14205"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOA53402.1"
                     /translation="MQSAQPWVISVTTATFQRDVLDQSRNVPVVVDFWAPWCGPCRML
                     GPLLEKIAKEKQGAFILAKVNTDECPDLAAAFGVQGIPAVFAVRDGRIVDHFMGALPE
                     TDLRAWIDGVLPQRDEQLVAQADSLAEQDPHRAEELYRQALAENPKSIPARLGLAKLF
                     AGRRQFEELKTILQELSDMGVSGEEIERLRASVEFAESTSSTDEIAELQRQVASDPSN
                     HALRLQLAKALASRQHYQEALDEALAVVQAAQGELRDDARKLMIQVFSLLGNEHPLTA
                     EYRRRLTMALF"
     gene            1237..>1786
                     /gene="uvrA"
                     /locus_tag="PKI05_14210"
     CDS             1237..>1786
                     /gene="uvrA"
                     /locus_tag="PKI05_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650187.1"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. UvrA is an ATPase and a
                     DNA-binding protein. A damage recognition complex composed
                     of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities.
                     When the presence of a lesion has been verified by uvrB,
                     the uvrA molecules dissociate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase UvrA"
                     /protein_id="HOA53403.1"
                     /translation="MNTQPQISIPSPSAIRIRGARVHNLKNIDVDIPHERLVVLSGPS
                     GSGKSSLAFDTIFAEAQRAYLETLSPYLRQFCLQLPQPDVDLIEGLPPVVAIAQWAGA
                     SQPRSTVGTITEVYDFLRLLYAKVGVLHCPHCAVPLGSQSPENIATELMRLPEGTRVM
                     ILAPFVFQKRGEHKEVFRHIQKL"
BASE COUNT          409 a          459 c          517 g          401 t
ORIGIN      
        1 taccacgtag caaagcttca tagatcgtgg acggttttga ccaaaagact gtcaggtccg
       61 caagtggtct ggcgtggctt ggcggacaag gagacgtcat ggcgatcttc gcccattttt
      121 gggatgagtc catgcccggc gtcgggcctt tacgcaggcg ttgcggctgc cataaacttg
      181 atctatgact ccgtcagagg atcttggatt aattctgtcc acgatttgct cagaacctcc
      241 gaattcggca aaagcatgag gtgaaagaat gcaatcggcg cagccgtggg tgatttccgt
      301 aacgacagcg acgttccagc gggatgtgct cgatcaatct cgaaacgttc cggtggtggt
      361 cgatttctgg gcgccgtggt gtgggccctg ccggatgctg ggacccctgc tggaaaagat
      421 cgccaaagaa aaacaagggg cgtttatcct ggcgaaagtc aatacggatg agtgccccga
      481 cctggctgcc gcatttgggg ttcaagggat tcccgctgtg tttgctgtgc gggacgggag
      541 gatcgtcgac cattttatgg gtgcgctgcc tgagactgat ctgcgggctt ggatcgatgg
      601 ggttttaccg caaagagatg aacagttggt ggcccaggcc gatagccttg ccgagcaaga
      661 cccacaccga gcagaggagt tatatcgcca ggccctggcc gaaaatccaa aaagtatccc
      721 tgcccgtctc ggtctagcca aactcttcgc cgggcgccgc cagttcgagg agctcaagac
      781 gatcctgcaa gagttgagtg atatgggcgt aagcggtgaa gagatcgaac ggctgcgcgc
      841 atccgtcgaa tttgcggaat cgacaagttc aactgacgaa attgcagaac tccaaaggca
      901 agtggcaagc gatcctagca atcacgcctt gcgtcttcag ttggctaagg cactggcctc
      961 ccgtcagcat tatcaagagg ccctagacga ggcgctggct gttgttcagg cggctcaagg
     1021 cgagttgcgc gatgatgccc ggaaattaat gattcaggtt ttcagtctgc tgggtaatga
     1081 acaccctttg acggctgaat accgccgacg gctgaccatg gcgctgtttt aacaccatgg
     1141 attggtgaaa atcacatctc caagctgcca tcctggaagt gaacctggcg aatacaaggt
     1201 aatagggcaa cgggaatagt cgcattcgta aaaccggtga atacgcaacc acaaatcagt
     1261 attccaagcc catccgccat tcgcatccgt ggggcgcggg tccataatct gaagaatatc
     1321 gacgtcgata ttcctcatga acgtctggtg gtgctcagcg ggccaagtgg ttcaggaaaa
     1381 agctccttag cgtttgacac catcttcgcc gaggcccagc gggcatatct cgaaacattg
     1441 tcgccatatc ttcggcagtt ttgcttacag ttaccacagc ctgatgttga tctgatcgaa
     1501 gggcttcccc cggttgttgc catcgcccag tgggcagggg ctagccagcc gcgcagtacg
     1561 gtgggaacga ttacggaggt gtatgacttt ttgcggcttc tgtacgcaaa ggtcggggtc
     1621 ctccactgcc cccattgcgc tgttcccctt ggctcacaat ccccggaaaa catcgcgacg
     1681 gaattgatgc gcctaccgga aggaacgcgt gtgatgatct tggccccgtt tgttttccaa
     1741 aagagagggg aacacaaaga ggtatttcgt cacattcaga agcttg
//
LOCUS       DAOPTR010000890         1784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_51801_length_1784_cov_2.966455, whole genome shotgun sequence.
ACCESSION   DAOPTR010000890 DAOPTR010000000
VERSION     DAOPTR010000890.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51801_length_1784_cov_2.966455"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..1403)
                     /locus_tag="PKI05_14215"
     CDS             complement(210..1403)
                     /locus_tag="PKI05_14215"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOA53404.1"
                     /translation="MLKTWSFHLPTFVRFGPGLIRKLGELAQEFGWNALLVGYKDQTG
                     MEEVYQRAQRSLRSARMHIFTFFEVGPEPDADLVPRGVEMYKKNRCEVVIGLGGGSVL
                     DVAKAIGALVKMEGVPWDFANANEGHRRITESAPIIAVPTTAGTGSEVTAVAVLSFSG
                     KGRHPDMPLKAALVGEGLHPKIALIDPELTVGKPAPVTAACAADALLQAVEACVSRMA
                     NPFSTVLAGRAACLIAQSLRTALSQPQDIAARSDLALGACFSGAAYDVTGVTVAHAMA
                     QALGAVFGVPHGLAVAIAGPVALRFLQQTCAEPFAELGRQCHILANTPEEQAGRFLDT
                     VEQLFRDVGLPDKYTIPANAPKDYLDRLVHHALQATPEACTLTPVKVDESVLRDLFQK
                     ILSPA"
     gene            complement(1476..>1784)
                     /locus_tag="PKI05_14220"
     CDS             complement(1476..>1784)
                     /locus_tag="PKI05_14220"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOA53405.1"
                     /translation="KILEGMGKLGCDIVDLDFLVPMEAGRQAMGPDQVLLGNIDPVKV
                     LRNGTPELVAEKLAECHRASGKRYIVGAGCEVPRGTPDANMFAMRDYAINHRPDDFIS
                     "
BASE COUNT          384 a          544 c          516 g          340 t
ORIGIN      
        1 ggcgattcct gatggcggaa gcgcggatag cgaatgcgaa cacgggcagg cacaaggcct
       61 gcccctacgg gtgatcggca agcgcatcca tgatgaatac acagccccac cttacggcga
      121 gaactcgcaa ggcggcgacc tcgcagctaa cttggcccca tgagccgcaa aatgcaaact
      181 agtgagacga aggccctgta ctgaccgggt cacgcggggc tgaggatctt ttggaagaga
      241 tcgcgcagga cgctttcatc gacctttacc ggcgttaagg tgcaggcctc cggtgtggcc
      301 tggagggcgt gatgcaccag ccggtctaaa taatcttttg gggcgttggc cgggatcgtg
      361 tatttgtcgg gtagccccac gtcccggaaa agctgctcga ccgtgtccaa gaatcgaccg
      421 gcttgttctt ctggggtatt ggccaggatg tgacactgcc gtcccaactc ggcgaagggc
      481 tccgcgcagg tttgttgaag gaatcgcaac gccacgggcc cagcgatggc cacggccaag
      541 ccgtgaggca ctccaaacac ggcccccaag gcctgggcca tggcatgggc cacggtgacg
      601 cccgtcacgt cataggcggc gccggaaaag cacgcaccca gggcgagatc ggatcgcgcg
      661 gcaatgtctt gcggctgact gagggccgtt ctcaggcttt gcgcgatgag acaagcagca
      721 cgaccggcca agaccgtcga gaaaggattg gccatccggc tgacgcaggc ctccactgcc
      781 tgcaagagag catccgcggc gcatgccgct gtcactggag ctggtttacc cacggtaagt
      841 tccggatcaa ttagggcgat cttgggatgg agaccttctc ccacaagggc tgccttgagg
      901 ggcatatccg gatgccgacc cttgcccgag aacgacaaca cggccacagc cgttacctca
      961 ctccccgtcc cagcagttgt ggggacagcg atgatggggg ctgattcggt gatcctccgg
     1021 tgtccctcgt tcgcgttggc aaagtcccat gggacaccct ccatcttcac caacgcgccg
     1081 atagccttgg ccacgtcaag aacactgccg cctcctaatc cgatgaccac ctcgcaccgg
     1141 ttcttcttgt acatctcgac gccgcggggc acgagatcgg cgtctggttc cggtccaacc
     1201 tcgaaaaagg taaagatgtg catccgcgcc gagcggagcg agcgctgtgc ccgttgataa
     1261 acctcttcca tgccggtctg gtctttgtaa ccgaccagca acgcattcca accgaactct
     1321 tgggctaatt ctcccagttt tcggatcaac cctggtccaa agcgaacgaa cgtcggcagg
     1381 tgaaacgacc acgtcttaag catggaagca ctcctatagg ccgatgcggt caatgacact
     1441 atcaagcaag gcttacaagg tgtgtcaaat tgattttagg agataaaatc atccggacga
     1501 tggttgatgg catagtccct catcgcgaac atattggcat ccggcgtccc tcgggggact
     1561 tcgcaaccgg cccccacgat atatcgcttt cccgaagcac ggtgacactc ggcgagtttc
     1621 tcggcgacca attctggcgt gccgttccgt aggaccttca ccgggtcaat attcccgaga
     1681 aggacctgat caggccccat ggcttggcgg ccggcctcca tgggcacaag gaaatccaaa
     1741 tcgacaatgt cgcaaccgag ctttcccatg ccctccagaa tctt
//
LOCUS       DAOPTR010000891         1784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_51806_length_1784_cov_2.489879, whole genome shotgun sequence.
ACCESSION   DAOPTR010000891 DAOPTR010000000
VERSION     DAOPTR010000891.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51806_length_1784_cov_2.489879"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PKI05_14225"
     CDS             <1..422
                     /locus_tag="PKI05_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53406.1"
                     /translation="DYNGSVNELNAFVQNWEASQVKTDPGQIVTQLDGSKVFHFIGQL
                     REFAERVSEDAERPSSVVFQNLDNGVKQLGESLAQAYTDGRTQIKELLDRFDQELSRA
                     ADQATAVKLRYERRQLGRYTAFPFEEISRQVIAGLDR"
     gene            1063..>1784
                     /locus_tag="PKI05_14230"
     CDS             1063..>1784
                     /locus_tag="PKI05_14230"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOA53407.1"
                     /translation="MEQDRLPVDSRLVVVLHNPVAGRRPSDRRIEQLVNALREKGFSL
                     HVSTNLSQATDLAYREFEKGNLRCLVGAGGDGTARELANRVQPGVPVCFFPTGTSNLV
                     ASYLGMKKRIPFVVSLIETGATVCLDAGLANGRIFLAMVSCGFDAAAVEMAHRKRVAG
                     HRQGRGGFSGFIIPICRLINTYPFPEMTVEVSDGYEGGSSGEPSSSQRTIKGRWVFAF
                     NLPKYGWGLPIAPQANGMDGLL"
BASE COUNT          400 a          434 c          536 g          414 t
ORIGIN      
        1 gggattacaa cggtagtgtt aatgaactca atgcctttgt ccaaaattgg gaagcctctc
       61 aggtgaaaac tgatcctggt cagatcgtta cccaactgga cggcagcaaa gtgtttcact
      121 ttatcggaca gctgcgagaa tttgcagaac gggtttctga ggacgcggaa cgaccgagta
      181 gcgtcgtatt tcagaatttg gacaatggtg tcaaacagtt gggggaatcg ctcgcccaag
      241 cctataccga cgggcgaacg cagatcaaag agctgttgga ccggttcgac caggagttga
      301 gtcgggcggc cgatcaagcg acggcagtga agctccgcta cgaacgtcgc caattgggac
      361 gctacaccgc atttcctttc gaagaaatct cccgacaggt tattgcgggg ttagaccgtt
      421 agcgtcgtca ggcggggagg cccacaactt cgggacgccc tttgtggcgc tcattgttga
      481 gggggcgcca gatgaggctt tcttcggtgg aaacacacgg aggaggagaa aatcactgga
      541 ctgataaaaa aagtgggctt ccggcgtgcc acccccacag tggccacgtc atgcggtagg
      601 gcaaggtacc cgcatgacac cctctcgccg gaaaccccac gggccagcct gagttgtcac
      661 tagattctcg agcaactcct gttccggtta tcacggaaac acggttgttc gatataagtg
      721 tattattaaa aaggatttat ggattagccg taagattgtg gccaggtcgc gaaaccggcg
      781 gtggtgtatt attttgcaac acttgcagca atcggctacg aaaagtggct tttgcaggcc
      841 gcacccggac atccgattga tcaattttgc tacactaaag agtatgtgcc ggaacacact
      901 aatcgtccca cctcagtggg ttgatcgggt ttgcggagat ggttcggtaa gctagctccg
      961 tcgtacggtg gcaggaggcg gaaaaagggg tattatagca tttgtggttg taatgattcg
     1021 ttttgggcaa agctggtccc tcgttcgttc aggcgatgct taatggaaca ggatagactc
     1081 ccggttgact cgaggcttgt cgttgtcttg cataacccgg tcgcagggcg gaggccgtcc
     1141 gatcggagga ttgagcaatt ggtgaatgcc ctccgagaaa agggtttttc cctccacgtg
     1201 agcacgaatc tttcccaagc gaccgatttg gcctatcgag agtttgaaaa aggcaacttg
     1261 cgctgcctgg tgggtgccgg cggggatggt acggcacgcg agttggccaa tcgagttcaa
     1321 ccgggcgtcc ccgtctgttt tttcccaacg gggacgtcga acttagtggc cagctatctt
     1381 ggtatgaaaa aacgcatccc gtttgtcgtg tcattaattg aaacgggagc cacggtgtgt
     1441 ttggatgctg ggctcgccaa cggacgaatc ttcctcgcca tggtcagttg cggttttgat
     1501 gccgcggccg tggaaatggc gcaccggaaa cgggtggcag gacaccgcca aggccgggga
     1561 ggattctccg gctttatcat ccccatctgt cgcttaatta atacctatcc atttcccgag
     1621 atgaccgtgg aagtgagcga tggctatgaa ggtggttcga gcggggagcc ttcctcgtct
     1681 cagagaacca ttaagggccg ctgggtgttt gcgttcaatt tgccgaagta cgggtggggg
     1741 ctccccattg cccctcaagc caacggcatg gacgggcttc tgga
//
LOCUS       DAOPTR010000892         1783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_51838_length_1783_cov_3.482639, whole genome shotgun sequence.
ACCESSION   DAOPTR010000892 DAOPTR010000000
VERSION     DAOPTR010000892.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51838_length_1783_cov_3.482639"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /locus_tag="PKI05_14235"
     CDS             complement(<1..1415)
                     /locus_tag="PKI05_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53408.1"
                     /translation="MRVSSLFGFRQGGVSRGQQRKAKRSRRTRRQLLLEPLEQRHLLS
                     VVTITANDDPGGNGANNGIADLFRLVRNADDLEVYVNGNFVTSAPLAGLTAVQVGGSS
                     DADELQIDFSGGTFSTTIQFDGEALGLNVAPQDSLTLLGDPGAPIGRVTYAAGPLFGG
                     GADDGFIILDPDDNNGPGGGWNVGFATWNGDELMIQFANLSPVYDVVPAAQLDVFATH
                     AAETISILDGILVSGYQTGKVSASTFEDYFFGNKTRVTVNSLNGGDTFILNYNVTVPG
                     LQLLDLYGNELTGGTLNPDDAQAETFQILATGPGTVANRMYGQRGAEYYTNSLTSYLD
                     PIQSNVQMHGGEGISIIDLSDVNDPTGDNIFIWGNKIVGAATGTIELYFATIEDIRFE
                     ATSGDDVITVLSTFADFQYRISGGPGNDTFNVGSYDTDPSPGVSLAWGPYGTGLNTIG
                     QIKGQLTIIPGPAWDAGDDTL"
BASE COUNT          442 a          503 c          452 g          386 t
ORIGIN      
        1 tacagcgtat catcgccagc gtcccacgcg ggtcccggga taattgtcag ctgaccctta
       61 atttgtccga tcgtgttcag tcccgttccg tacggtcccc aagccaagga gacaccaggc
      121 gagggatctg tgtcgtaaga accgacgtta aatgtgtcgt taccaggccc accggagatt
      181 cggtactgaa aatcggcaaa cgtggaaagc accgtgatga catcgtcgcc ggatgtcgcc
      241 tcaaaccgga tgtcttcgat cgtggcaaag tacagctcaa tagtgcccgt cgccgctccc
      301 acaatcttgt tgccccagat gaagatgtta tcaccagttg ggtcgttaac atcggaaaga
      361 tcgatgattg aaataccctc tccaccgtgc atttgcacat tactttggat gggatcgaga
      421 tagctggtga gactattcgt gtagtattct gcaccgcgct gaccatacat gcgattcgca
      481 acggtgcccg ggccagtcgc caggatttgg aatgtctcgg cctgggcatc atccgggttg
      541 agcgttccac cggtcaattc atttccgtaa aggtccagca attgaaggcc gggcacggtg
      601 acgttgtagt taagaataaa cgtgtctccg ccattgagac tgttcaccgt cacacgggtc
      661 ttgttgccga agaaatagtc ttcgaacgtc gatgcagaaa ccttgccggt ttgataacca
      721 ctgaccagaa tgccatcaag aattgaaatt gtctcggcgg cgtgggtggc gaagacatca
      781 agctgggcgg ctggcaccac atcataaacc ggcgagagat tggcaaattg aatcatcaac
      841 tcgtcgccat tccaagtcgc aaagccaacg ttccaacctc cgccgggtcc gttgttatca
      901 tccgggtcaa gaatgatgaa cccgtcgtcc gcaccaccgc cgaataatgg ccccgcggcg
      961 taagtcactc tgccgatagg ggcaccaggg tcacccaaaa gcgtgagcga atcttggggg
     1021 gcaacattaa gtccgagggc ctctccatca aactgaatcg tcgtcgaaaa cgttccaccg
     1081 gagaagtcga tctgtagttc gtctgcgtcc gaggaaccac cgacttgcac tgccgtcagc
     1141 ccggccaaag gagctgaagt gacaaagttc ccgttgacgt aaacttcgag atcgtcagcg
     1201 ttgcggacca gccggaataa atcggcgatt ccgttattgg caccatttcc gcccgggtcg
     1261 tcgttggccg taatggtaac cacactcaac agatggcgct gttccaaggg ctccaaaagc
     1321 aactgccttc gcgttctgcg agagcgtttg gccttccttt gttgacctcg tgaaacaccg
     1381 ccttgccgaa aaccgaagag gctgctaact cgcatggttg ctcactccac aaaagaaatt
     1441 aggccgctgc taaaaacccc cctgaaacgc agacttggtc aaaactcaac gtcaaccgcg
     1501 attgtgttcc gttcgaaaag cgagagtggc gacttgccca aaaaacttct caagtcgcgc
     1561 aaatccttaa aatccgcaaa gtcgacaaag ctcctcaaat cggcaaacct gacaaaattt
     1621 ataatctgcg gcacccccgt tgtcaacaac tttcttggcc agccccgaat tagggcgaat
     1681 accggaaaaa ccaggccccc gtccattggg gtatatgccc gagttggcct gctcagcgga
     1741 gggaccaaaa aaacctttgg tgtccgttct ctcagggcac ctc
//
LOCUS       DAOPTR010000893         1781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_51913_length_1781_cov_4.242178, whole genome shotgun sequence.
ACCESSION   DAOPTR010000893 DAOPTR010000000
VERSION     DAOPTR010000893.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51913_length_1781_cov_4.242178"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..1415
                     /locus_tag="PKI05_14240"
     CDS             240..1415
                     /locus_tag="PKI05_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="STN domain-containing protein"
                     /protein_id="HOA53409.1"
                     /translation="MRRPVIRSQALLIGPVLALVCVGISPAWCQEGPSFVIGNEFLRR
                     LDTPTGIFWTDVPLREGLLRLGRTHQVAIVLDRRCDPDQELTLTVRDRPLREVIAAIA
                     EACGVQSVVVSPIVYVGPPEAVQRLESLMVARRQEALKLGKVGLAGLRPLAVSWPRLT
                     TPRQLVTLWLQEANWRLTNPDEILHDLWPEGSLPPLTLVDRLTLVLAQFDRTFELNES
                     GEIRIVPITNTMAVARQFSVGPQARILAQQWQKMFPDCKVTTFGQDVVVEGPRETLDK
                     LADLLRTQKGPTAPRGSDGPPPTTPEARDPFEQRRFTVREGRGTLEGVIRQLAQQLEM
                     QVDFNRQSAIEAGIRLDQPIRFQVQNGTIDELWRAVLDPHGLTFRRVGRSITIYPKR"
     gene            complement(1441..>1781)
                     /locus_tag="PKI05_14245"
     CDS             complement(1441..>1781)
                     /locus_tag="PKI05_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53410.1"
                     /translation="VSSDSTLGCPSCAPASSSPLQPSVDTSIPTLPSASPSDQPPATF
                     KQQSSSTTGSSFRPADSTSGGMNATGTSVPVNETKPLVRQALYEKPIPLVEEVADPPM
                     VDVTGWRRSQ"
BASE COUNT          355 a          506 c          532 g          388 t
ORIGIN      
        1 acatcggaat cgattgagaa atccggcagc gattcgagcc accctgaccc ccaacccctt
       61 tcccgccgaa cgggcgagag gagattatgg gataggctct aactaaacta caagaaagga
      121 atggccgtgg ccgatttggt caacaggtca gatcgtttct cctctttgcc ttttggcaga
      181 ggcggccttt gggccaagtt ggcgtgctcc ccgtggcatt gtgatttgaa tattacgcga
      241 tgagacgtcc agtcattcga tcgcaagcct tattgatagg gcccgtcctg gcgctcgttt
      301 gcgtggggat aagccccgcc tggtgccaag aaggcccatc ctttgtcatc ggcaacgaat
      361 tcttgcggcg gctcgacact ccaacgggta tcttctggac cgatgtgcct ttgcgggagg
      421 gccttctccg cctgggacga acgcatcaag tcgccatcgt cctggatcga aggtgtgatc
      481 ctgaccaaga gctgacgttg actgtgcggg atcgacccct gcgggaggtc atcgcggcca
      541 ttgccgaggc gtgcggagtt caaagcgttg tcgtttcccc gatcgtgtac gtgggtccgc
      601 cagaggcagt ccaacgcttg gaatcgctca tggtcgcgcg acggcaagag gccctgaagc
      661 ttggcaaggt ggggcttgcc ggactgcgcc cgctggccgt ctcttggcca agattgacca
      721 cgcctcgcca actcgttacc ctctggttgc aagaggccaa ttggcgattg acgaatcccg
      781 atgagattct tcacgacctc tggcccgagg gatcgcttcc ccctttgacg ctcgttgacc
      841 gtttgacgct tgtattggca cagttcgacc ggacgtttga actcaatgaa tcgggcgaaa
      901 tacggattgt gcccattacc aacacaatgg ccgtggcgag acagttttcc gtcggacctc
      961 aagcgcggat attggcccag caatggcaga aaatgtttcc cgattgcaaa gtgacaacgt
     1021 ttggccagga tgttgttgtc gagggaccac gcgaaacgct ggacaagctg gccgatcttt
     1081 tgagaaccca aaaaggcccg acagccccgc gcggctccga tggtccaccg ccgacaaccc
     1141 ccgaggcccg tgatccattt gagcagcgcc gatttaccgt gcgggaaggc cgaggcacgc
     1201 tggaaggcgt catccgccag cttgcgcaac aattggagat gcaggtcgat ttcaatcgcc
     1261 agtctgccat agaggcaggg attcgactgg atcaacccat ccgctttcag gtccagaatg
     1321 ggacgattga cgaactttgg cgggcagttc tcgatcccca cggactaact tttcgccgcg
     1381 tcgggcgatc cataacgatc tatcccaaac ggtgagtacc cgcgggaatc agatcacttg
     1441 tcactgactc cgccgccagc cagtgacatc aaccatcggc gggtcggcca cctcctcgac
     1501 gagtgggatc ggcttttcgt aaagggcctg ccgcaccagg ggttttgttt cgttcaccgg
     1561 gacacttgtg cccgtggcat tcattccccc gcttgtgctg tcggcgggcc tgaaggacga
     1621 tcccgtggtt gagctgctct gttgtttgaa tgttgccggt ggctgatctg aaggcgatgc
     1681 cgacgggagt gtgggaattg aggtatcaac gctcggttgc aacgggctac tcgaggcagg
     1741 ggcacatgag ggacatccga gggtactgtc gcttgatacg g
//
LOCUS       DAOPTR010000894         1777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_52077_length_1777_cov_2.818235, whole genome shotgun sequence.
ACCESSION   DAOPTR010000894 DAOPTR010000000
VERSION     DAOPTR010000894.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52077_length_1777_cov_2.818235"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PKI05_14250"
     CDS             complement(<1..467)
                     /locus_tag="PKI05_14250"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HOA53411.1"
                     /translation="MRKYSYGLAKACTFGAISALLLTTIVDSFAYSQDFFGALNQNKF
                     RVTGALTRVEPVGYILLVKAEIEPGWHIYSLTQKGGPVATKIDLAFPEGVAPAGAFVV
                     FPDPERKTEAAFPGLIVETHSGTVVWAVRLGIEHTLSPSKLTIEGSLKAQPCRA"
     gene            1011..1358
                     /locus_tag="PKI05_14255"
     CDS             1011..1358
                     /locus_tag="PKI05_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOA53412.1"
                     /translation="MKLILAIVQPPKLESIRGALERIEVSRLTLCDAQGYGRQRGHTA
                     SYRGHEYKAKLLRKVALEIVVNDDFLDRTINTIAHVARTGSEGAIGDGKIFVLPVEEA
                     IQIEDGTRGPGAV"
     gene            1377..>1777
                     /locus_tag="PKI05_14260"
     CDS             1377..>1777
                     /locus_tag="PKI05_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53413.1"
                     /translation="MTFRGIFLDRTSVSTTWKRKKFERSWDLACYRLVMPLFYQMIHG
                     RIPMETSDHSSEGSFERIDLTSAPDCGSDVRRGKGRPYLGILFRCCGIYLRIYLNRDG
                     TAFEGRCPRCLRVVRIGVSPDGSDDRFFSAF"
BASE COUNT          426 a          467 c          454 g          430 t
ORIGIN      
        1 gcccgacatg gttgtgcttt aaggcttccc tcgatagtga gtttcgatgg ggaaagagta
       61 tgctcgatac ccagtcttac ggcccacaca acggtcccag aatgggtctc tacaattagc
      121 cccggaaaag ctgcctctgt cttgcgttcc ggatcaggaa agacgacaaa cgcacctgct
      181 ggagctaccc cctcgggaaa agccaaatca attttcgtcg caacaggccc gcccttttgc
      241 gttagtgagt agatatgcca acccggttcg atttcagcct tcaccaggag gatgtaacct
      301 acgggctcga cgcgcgtgag tgcaccggtc actcgaaact tgttttggtt aagtgcacca
      361 aaaaagtctt gactgtaagc aaacgaatca acaattgtgg tcagcaacag cgccgagatt
      421 gccccaaacg tgcaggcttt tgccagaccg tacgagtact ttctcatcct tgttatctgc
      481 atgaaacaac cgtctcctgc gactccgcaa ggaaaatttt cacgatggat aaagccaaga
      541 aggcttacta tgccggccat tcgcaccaaa ccgatgcgta caaggcattt tctcatctta
      601 tgtgtaagga tggtcgaccg gccaggggcg ttacaatccc gctttcatct tcccatcttg
      661 aaaatctcga cggttctcat aggcggtctt catgagcaat gcccactcgg catttgagag
      721 gtaaagcccg gacctactat gttgcccaag ctgtgtatat ttcctggtcg ttaacagcgg
      781 cgagtttcgc aaggaccacc cagctttgcc ccctggcagt ttcactttcg cacaaagacg
      841 atcattctcg gtcagaatga atttgaatca acaaaactgc ctctctggcc attgtgtgcc
      901 gaaaacaaac acagcgcttg acgcagtagc aggcaagaat atgattggac ggacatcaag
      961 ccgatattcc acatgattgt ttgtttcggg aagtttcgaa aagagacaca atgaagctaa
     1021 ttttggctat tgtccaacct ccgaaactgg aatcgatccg aggggccctc gagcggatcg
     1081 aggtctcacg tctcacgctc tgcgatgccc aggggtatgg gcgacagcgc gggcacacgg
     1141 cgtcgtatcg gggacatgaa tacaaggcaa aactcctgcg aaaggtggcc cttgagattg
     1201 tggtcaacga tgactttctc gaccgcacaa tcaatacgat tgcccacgtg gcgagaaccg
     1261 gttcggaagg cgccattggt gacggaaaga tctttgtgct tcccgttgaa gaggcgattc
     1321 aaatcgaaga tggcacacgg ggcccggggg ctgtttgacg ccgaagaagt atcattttga
     1381 catttcgcgg aatctttctc gacagaacct cggtttccac gacatggaaa cgcaaaaaat
     1441 tcgaacgaag ttgggacctc gcttgctatc gcttggtaat gccgcttttt tatcagatga
     1501 tccacggtcg tattcccatg gaaacttcag accattcatc cgaaggctca tttgagcgca
     1561 ttgacctgac cagtgcgccc gactgtggca gcgatgttcg gcggggaaag ggtcgcccct
     1621 atcttggcat tcttttccgc tgttgcggaa tttacctccg catttacttg aatcgtgatg
     1681 gcacagcgtt tgagggccgt tgcccccgat gcctgcgagt ggtccgaatc ggcgtttcac
     1741 ccgatgggag cgatgatcgg ttcttttctg cgttctg
//
LOCUS       DAOPTR010000895         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_52141_length_1775_cov_3.590116, whole genome shotgun sequence.
ACCESSION   DAOPTR010000895 DAOPTR010000000
VERSION     DAOPTR010000895.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52141_length_1775_cov_3.590116"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PKI05_14265"
     CDS             complement(<1..538)
                     /locus_tag="PKI05_14265"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOA53414.1"
                     /translation="MATFLRGVTMSLAVAMLLVGLSSCCVAAEDSGLPWKIGLARKDI
                     TPQQPIWLAGYASRNKPSEGVLAPLYATALAIEDSQGNRGVLITADVIGFRKEVAEAA
                     ARAIMEKTGLAREQILFNWSHTHTGPVIGLSRPAGYSMGEEDQKVVETYTRGLIAQYA
                     DLAAEAIADLKPARLAWGV"
     gene            877..>1775
                     /locus_tag="PKI05_14270"
     CDS             877..>1775
                     /locus_tag="PKI05_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53415.1"
                     /translation="MSCHRFDHAVRGLRLLVIATVVLGTVPWQAMGMELAKTGTRRTV
                     VSIDGTRWLINGQVTYPGSPAEGLLMNVRMVNATFEDLNRQDFDPEANTERFLAHLPD
                     YVAHGIRAITLNFQGGHAGYEGPLNSAFNPDGSLREEPLKRIKRVIEACDQQGVVVIL
                     GCFYQRQDQILKDEEAVKTALVNAVRWIEQQDFTNVVLEVANEFPHRGFDHPILRKPE
                     GQVELIRLAKKTNPRLLVSTSGIGDGRFPDVVAEVADFILIHFNGVPVEAIPERIASL
                     KRFGKPIVCNEDDKTGSVAAQAA"
BASE COUNT          430 a          507 c          477 g          361 t
ORIGIN      
        1 caacgcccca cgcaagtcgg gccggcttca aatcggcgat ggcctcagcg gccaggtcag
       61 catactgggc aatcagcccg cgggtatatg tttccacaac cttttggtct tcctcaccca
      121 tgctgtaccc ggccgggcgc gacaaaccga tcaccggtcc tgtatgcgta tgcgaccagt
      181 tgaaaaggat ctgctctcgt gccaggcccg tcttctccat gatcgcacgc gccgctgcct
      241 cggcaacctc tttgcgaaag ccgatgacat cggctgtgat tagcacgccg cggttgcctt
      301 gactatcctc aatcgccagc gccgttgcgt agagcggcgc gaggacccct tcggagggct
      361 tgttgcgcga ggcgtaacca gccaaccaaa tcggctgttg aggcgtgata tccttcctcg
      421 ccaagcctat cttccacggt aaaccgctgt cttctgcggc aacacagcag gaacttaacc
      481 ccacgagcag cattgcgact gccaaagaca tggtcacgcc acgcaaaaag gtagccatcg
      541 cagcacctcc tcaggcaaaa cccaccaccc aacatccaaa aagcaacgca acttgctaat
      601 catcttatct caaaaaggtt cctcaaacca tcagcaaccg cggccaacga tgggttggag
      661 aagctccgca caacctagcc ggatcatcgg tacacaaaaa cagccaacaa gccaggagga
      721 gcaacccggg cggcttggca atcgtcgtca ttcggtcccg atatagttga tcatggcgat
      781 tgaatcacct aaagtgatgg catccttcgg aacatcccac aggacgtgcg aagccacatg
      841 tttgctactt tagactcgcc acgggaggag atgacaatga gttgccaccg gttcgatcat
      901 gcagtgcgcg gattgcggct attggttatc gccacggtcg ttctgggcac ggttccctgg
      961 caagccatgg gaatggaatt ggccaagaca ggcacccgac gtaccgtcgt ctcaattgat
     1021 ggaacgcggt ggctgatcaa tgggcaggtc acatatccag gcagtccggc cgaagggctc
     1081 ctcatgaatg tgcggatggt caacgcaaca ttcgaggact taaatcgcca agattttgac
     1141 cccgaagcaa atacggagcg ttttttagcc catttgcccg actacgtagc ccatggaata
     1201 cgtgcgatca cactcaattt ccagggtgga cacgcaggtt atgaaggacc gcttaactcg
     1261 gcgtttaacc ctgatgggtc gttgagagag gagccactta agcggatcaa acgggttatc
     1321 gaagcctgtg atcagcaagg cgtggtcgtc atcctggggt gtttttacca gcgacaggat
     1381 cagattctca aagatgaaga ggctgtcaaa acggccttgg tcaacgccgt ccgctggatc
     1441 gagcagcaag acttcaccaa tgtggtcctc gaagtggcaa atgagttccc ccatcggggg
     1501 tttgaccacc caatcctgag aaagcctgaa ggtcaggtgg agttgattcg gctggccaaa
     1561 aagacaaacc cccgcttact ggtctccacc agtggcatcg gggatggtcg atttcctgat
     1621 gtggtggccg aggtggccga ctttatcctg attcatttca atggggtccc agtcgaggca
     1681 atcccggaac gaattgcctc cctgaagcga tttggaaaac caattgtttg taacgaagac
     1741 gacaagacgg gaagcgtcgc ggctcaagcc gcaga
//
LOCUS       DAOPTR010000896         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_52147_length_1775_cov_3.337791, whole genome shotgun sequence.
ACCESSION   DAOPTR010000896 DAOPTR010000000
VERSION     DAOPTR010000896.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52147_length_1775_cov_3.337791"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="PKI05_14275"
     CDS             complement(<1..529)
                     /locus_tag="PKI05_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53416.1"
                     /translation="MLFVFLGLCLAGCPRPRQPETTSDTTSAEQTATLHRDELFEIAI
                     ANLNRLHQFDGTDMALQVADRLDQWIKTQPPLKEWEVDPLIKTLPEGFVNAELLQDLE
                     KLSLDREDGLFLQSAVWLRDIAAWARGEDPSELARACRLFDWVVLNIQLDSQDQARQL
                     PSGDLLVQKPWETLLL"
     gene            complement(631..1461)
                     /gene="kdsA"
                     /locus_tag="PKI05_14280"
     CDS             complement(631..1461)
                     /gene="kdsA"
                     /locus_tag="PKI05_14280"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOA53417.1"
                     /translation="MPNRIVSVGNYRCGPGESLLLIAGPCVIESESLTREVAFFLAEF
                     SKSRGIPIIFKASFDKANRTSLHSFRGPGLSQGLAILARVREESGLPVTTDIHEPAQA
                     QPVSEVCDLIQIPAFLCRQTDLLLAAAQTGKPVHVKKAQFLAPWDMRYVIEKLEGAGC
                     QNILVGERGTFFGYGRLVNDLRSIPILHDLGVPVIFDATHSVQEPGGQQGRSGGDRRM
                     VAPLARAAVAAGADGVFLETHPRPDEALSDGPNMVPLADLPRLVETLSELREWLKERI
                     "
     gene            complement(1454..>1775)
                     /locus_tag="PKI05_14285"
     CDS             complement(1454..>1775)
                     /locus_tag="PKI05_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53418.1"
                     /translation="GLLDLCVYGGGGFWRGLWYTVTTQVGIHERVCDFVRIWGTRFRV
                     TSNEPVPYQIDGDPAGYLPLDIGLAPGRVRIVVPERTAEMLQKQRRQSEAVSRVKNGS
                     LVCA"
BASE COUNT          399 a          549 c          452 g          375 t
ORIGIN      
        1 ccaaaagcaa ggtctcccag ggtttttgaa cgagcagatc accggatggc agttggcgag
       61 cttggtcctg ggaatcgagc tggatgttta aaaccaccca atcgaacaaa cggcatgccc
      121 tggccagctc gcttggatcc tcaccccgtg cccaggcggc aatgtccctt aaccaaacgg
      181 ccgattgcag aaagagcccg tcctcccggt ctaaacttag cttctccaaa tcttgcaaca
      241 gttcggcatt aacaaacccc tccggaagcg tcttgatcag cggatcgact tcccactctt
      301 tgagcggggg ttgggtcttg atccattgat cgagccgatc agctacttgg agggccatgt
      361 ctgtgccgtc aaattgatga agacggttca gattggctat cgcaatttca aatagctcgt
      421 cccggtggag cgttgccgtc tgttccgcgc tggtagtgtc gctggtcgtt tctggttgtc
      481 gcggcctcgg gcaccccgca aggcacaagc ccagaaaaac gaacagcaaa aatatccatc
      541 ctgccttcct gcgagagaaa acctccgaac acccggctcc tagccgtcgc cttagggctg
      601 gatacgaaat gccgcgaaac ttctttgtga tcatattcgt tcttttaacc attcacgcag
      661 ttcggacaat gtctcgacca atcgaggcag gtcggcgaga ggtaccatat tcggaccgtc
      721 gctcaaggcc tcatcaggcc ggggatgggt ttccagaaaa actccgtccg ctccggctgc
      781 cacggctgct cgcgccagcg gtgcgaccat ccgccgatct cctcccgatc gtccctgctg
      841 accaccgggt tcctgtacgc tatgagtggc atcaaagatc acgggaaccc ccaggtcgtg
      901 caggatgggg atactccgca gatcgttgac aaggcggccg tatccaaaaa acgttccccg
      961 ctctcccaca aggatgtttt ggcaacccgc cccttccaat ttctcaatca cgtatcgcat
     1021 atcccacggt gctaaaaatt gggccttttt cacgtgaacc ggttttcctg tctgcgccgc
     1081 cgcgaggaga agatcggttt gccgacagag gaaagcggga atttgaatga ggtcgcagac
     1141 ctcactcacc ggctgtgcct gagccggctc gtgaatgtcc gttgtgacgg gcaggccgct
     1201 ttcttcccga accctggcca aaatcgccaa tccctgggaa agccccggtc cgcgaaagga
     1261 atggaggctc gtgcggttag ccttatcaaa cgaggcctta aaaataatgg gaatccctcg
     1321 tgacttggaa aactcggcca ggaaaaacgc aacctcccgg gttaacgatt cactctcaat
     1381 gacgcatggg ccggcgatca agaggagcga ttcccccggc ccacaccgat aatttcctac
     1441 actgacgatt cgattaggca cacaccaaac tcccgttttt cacgcgcgac accgcttctg
     1501 actgccgccg ctgtttttgt aacatttccg ccgttctttc gggcaccaca attcgtaccc
     1561 ggcccggcgc cagcccaata tcgagcggca agtagcctgc cggatcgccg tcaatctggt
     1621 aaggcacagg ttcgttcgag gtgacgcgaa atcttgtccc ccaaatgcga acaaagtcac
     1681 agacccgttc atgaatcccc acctgcgtgg tcacggtgta ccacagtccg cgccaaaatc
     1741 ctccgccccc atagacgcaa aggtccagaa gcccg
//
LOCUS       DAOPTR010000897         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_52169_length_1775_cov_2.250581, whole genome shotgun sequence.
ACCESSION   DAOPTR010000897 DAOPTR010000000
VERSION     DAOPTR010000897.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52169_length_1775_cov_2.250581"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..481)
                     /locus_tag="PKI05_14290"
     CDS             complement(74..481)
                     /locus_tag="PKI05_14290"
                     /inference="COORDINATES: protein motif:HMM:NF028296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CzcE family metal-binding protein"
                     /protein_id="HOA53419.1"
                     /translation="MKAHFTKIALIAALSAAGATTAFAHTDYSEGGYSEAGSAHWLSH
                     VAKSRSQPTANQLAAYGYVASGAAGRVLNVDSGTKYINVARLETVQINVGGKSVTWKF
                     DTFGTAPFPLSKIISGADGVTVYITADPASRGG"
     gene            complement(700..1131)
                     /locus_tag="PKI05_14295"
     CDS             complement(700..1131)
                     /locus_tag="PKI05_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53420.1"
                     /translation="MKALTTIATIFLASVTLYGCAGQSHRLVSEDGSGRAVGSITHGG
                     QTDPTMFLEFGEKRFESRGFAISRSQNLGALREEYGFGSKHYDRVSTGTDPEHYRYSA
                     KPELRAADGTTMQCVLAWRAYEAPAGVCVSPDGKEVKFRGE"
BASE COUNT          375 a          515 c          468 g          417 t
ORIGIN      
        1 atgctactct cggcaacctt acagtttcgg aaccggacga tgacatttgc gtcatcaccc
       61 ggtaatctct ctgtcagccc ccccgagagg ccgggtccgc ggttatataa accgtaaccc
      121 catcagcgcc agagataatc ttggagagcg ggaacggcgc ggttccgaac gtatcgaact
      181 tccaagtgac gctcttgccg cccacgttga tttggacggt ctcaagacgt gcgacattga
      241 tgtatttggt accactatcg acgttgagca cacgaccagc ggctccggac gctacataac
      301 cgtaggcagc aagctgattg gcagtcggct ggctcctgga tttcgcgacg tgcgaaagcc
      361 agtgtgcgct tcctgcttca gagtatcctc cttcagagta gtctgtgtgg gcaaacgccg
      421 tcgttgcacc tgccgcactc aaggcggcga tcagtgcgat tttcgtaaaa tgggccttca
      481 tggttttctc cttgtgacat ccggttgatc gcaccggaca gttatcgtcc ggcggtgatg
      541 tcatcttagg cgtcgcatgc caacagaacg ctgaccgggg aattacatct tggtcaggca
      601 ttcgtagcca ataggatggt cgagcagatt tttgcaggaa acgtgtccaa tctggttttg
      661 gcgccaagcc atcggtgcgg caaccgaagt cacgtcggct tactcaccac ggaacttgac
      721 ttcctttcca tcgggggaca cgcaaacccc ggccggggct tcatacgctc gccatgccaa
      781 tacgcattgc atcgttgtac cgtctgcggc gcgcaactct ggcttcgcag agtaccgata
      841 gtgctcagga tctgtccccg tggaaaccct gtcgtagtgc ttgctgccaa atccgtattc
      901 ttcccggagt gcgcccagat tctggcttcg gctgatggca aacccacgag attcaaatcg
      961 cttctcgcca aattccagaa acatggtcgg atcagtttgt ccgccgtggg tgatcgaccc
     1021 aacggcgcgt ccagacccat cctctgacac gagccgatgg gattgcccgg cgcatccgta
     1081 aagcgtgacg gatgccagaa agatagttgc aattgtcgtt aatgccttca tatagacctc
     1141 caaatttcaa tcaaaacaaa ccgccactgc tggcttctag ggggcgcgta agcagtacaa
     1201 taacctttgg ttatccctcc tgtcctgtat taacagattg gcgaatgcac gggtggctga
     1261 ggcttctggc taattagaca tccaccacag atactacggt cggcaacctt acggtttccg
     1321 aaccgtacaa taacgtttgt gtcaccatcc ggtaatctat cggtcagctt ccttggccgg
     1381 ccgggttttc ggccacatga accgtaacac tctcgatccc ggagacaatc ttggagagcg
     1441 ggaacggcgc ggttccgaac gttcgaactt ccaggcgatg ctggtgccgc ccatgtcgat
     1501 ccaaacggtt tcgagacgtg agacattcag gtacttggtg tcgctcgaca tccacccagc
     1561 gagctgattt gctgtgccgc ccggctactc gttatcgtcg atcttttcga cgcaatgaac
     1621 ttcaaaacgg aaacggctcc cgaaggagcc gctttgcgca cgggtgggcc ggttgttcag
     1681 cctcccgagt gtccctgctt gagcagggaa tcaaggcgca tgacctcgtc gccgtgcatc
     1741 atgatcttct ggccatcctt ggtttccatc accac
//
LOCUS       DAOPTR010000898         1772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_52285_length_1772_cov_3.026791, whole genome shotgun sequence.
ACCESSION   DAOPTR010000898 DAOPTR010000000
VERSION     DAOPTR010000898.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52285_length_1772_cov_3.026791"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /gene="der"
                     /locus_tag="PKI05_14300"
     CDS             complement(<1..1316)
                     /gene="der"
                     /locus_tag="PKI05_14300"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOA53421.1"
                     /translation="MAGFVPNGVFAKSGFGEEMAIPKVAIVGRPNVGKSSLLNWLVGR
                     RVSVVDARAGVTRDRVTALAKLDERLVELIDTGGIGLVDVDQLEDHVTRQIEIALHEA
                     DLILFVVDTRAGLTALDEEISRRLHTLQRPVICVPNKTDAPSLEPAAQEFSSLGWPIV
                     PVSAEQRRGKSELITAMLDLLPQELSEDDKAAIEPEMKIAVVGRRNVGKSTFINTLAR
                     EERMIVSEIPGTTRDSVDVRFEMDGRVFVAIDTPGLRRRKSVRENVDFYGVCRAEASV
                     RRADVVLLFFDATQDISGVDKQLAAYIAEQYKPCIFVVNKWDLMVGQIPTDVWVDRLA
                     KAFGTMSYVPVAFITGKTGKNVKRLIELAQSLFKQSRQRVSTGRLNRVLQRLLKSNPP
                     PLVGTKQVKIYYGTQVAVQPPTIVLVCNEPSLISSSYQRYLLNAMR"
BASE COUNT          391 a          514 c          442 g          425 t
ORIGIN      
        1 tccctcatcg cgttcaacag atatcgttga taactggatg aaatgagcga cggttcatta
       61 cacacgagga caatcgtcgg cggttgaacc gccacctggg tcccatagta gattttgact
      121 tgcttggttc ccaccaaagg tggtggattg ctcttgagaa gacgctggag taccctgttg
      181 agacgtccgg tgctcacccg ctgccgagac tgtttaaaca acgactgggc aagctcgata
      241 agtcgtttta cgttctttcc cgttttgccc gttataaaag ccacgggcac ataactcatc
      301 gttccgaagg ccttggccag acggtccacc catacatccg ttggtatttg ccctaccatg
      361 aggtcccact tgttgaccac gaaaatgcag ggtttgtact gttcggcaat ataagcggcc
      421 agttgtttat cgactccgga tatatcctgt gtggcatcga agaaaagcaa aacgacatcg
      481 gcgcgtcgaa cacttgcctc ggcgcggcag acgccgtaaa agtcaacgtt ttcgcgcaca
      541 ctcttgcgcc gcctgaggcc cggcgtatca atagcgacaa agacccgccc atccatttca
      601 aagcggacat ccacactgtc gcgagttgtg ccgggtattt cgctgacaat cattcgttct
      661 tctcgtgcca gtgtgttgat aaacgtactt ttgccgacat ttcgccttcc gacgacggcg
      721 attttcatct ccggttcgat ggccgcctta tcatcctcgc tgagttcctg aggcagaaga
      781 tcgagcatgg ccgttatcaa ttcgctcttg ccgcgtctct gttcggcact tactggaacg
      841 atcggccaac ccagcgaaga aaactcttgc gctgcaggtt ccaggcttgg ggcatcggtc
      901 ttgttcggga cacaaatgac gggccgctga agggtgtgca gccggcggga aatctcctcg
      961 tccagcgccg ttaatcccgc tcgggtatcg acaacaaaaa gaatcaggtc tgcctcgtgt
     1021 aaggctatct caatctgtcg ggtcacatgg tcctcaagct gatcgacatc caccaggcct
     1081 atgcctcccg tgtcgatcaa ttccaccaat cgctcatcca gtttggccaa ggccgtaact
     1141 cgatcgcgcg taacacctgc ccgcgcatcc accacggaca cacggcgtcc gacgagccaa
     1201 ttaaggaggc tcgactttcc cacattggga cggccaacga ttgccacttt gggaatagcc
     1261 atctcttcac cgaaacccga tttcgcaaaa accccgttgg gcacaaatcc ggccaccggg
     1321 cacatttcca atcttctaag gcttatcccg aaatcttccc tcgcccgttc ggcgggagag
     1381 gggccggggg tgaaggtggc tcaaatcgtt gctggttttc tcgatcgact ccgacgatct
     1441 cttgccctca ccccaaccct ctcccggaga gagagggcga ctttttggga taggctttaa
     1501 atcaacttga gtctgtcaca actggccaaa actttccgcc cgttctgtcc tcagccattg
     1561 attgtaccgg agagttcaca ataagtttat tcacggcaaa ttggttttac tgcatgggaa
     1621 tgacctgaga acgtttttca ttgtcgaggt ggaccatcgc gtaacgaaga gtagccttgt
     1681 ggttctcacc cgaggctgat cagcggccca tccgcggaac acctggggca gcgcaatctg
     1741 ccacacgact ttatcgtacg tatgcaactt gg
//
LOCUS       DAOPTR010000899         1771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_52318_length_1771_cov_3.185315, whole genome shotgun sequence.
ACCESSION   DAOPTR010000899 DAOPTR010000000
VERSION     DAOPTR010000899.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52318_length_1771_cov_3.185315"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PKI05_14305"
     CDS             complement(<1..902)
                     /locus_tag="PKI05_14305"
                     /inference="COORDINATES: protein motif:HMM:NF019219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HOA53422.1"
                     /translation="MNKTVTSVLCAVILWGGLGFAADPISLYESPTPAQPNGELDRIV
                     FAKLQTLGVQPILCSDAVFIRRAFLDMTGTIPTAQEVREFLDDQTPDKRARLVDRLME
                     RKEFLDYWSMKWGDALRIKAEIPVNLWPNGARAFHRYIWASLASNKPYDQFVREMLTA
                     SGSNFRDGAVNFYRAVQDRSPTGIASTVALTLMGVRTDHWPKERLEGMAVFFSQIAYK
                     PTSEWKEQVVFWDPWGLRLVPTNFVPGREAPGTAPRKSEDPIVTPTPADISQEPLVGV
                     FPDGTKVTIPADRDPREVFADWLIR"
     gene            complement(895..1086)
                     /locus_tag="PKI05_14310"
     CDS             complement(895..1086)
                     /locus_tag="PKI05_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53423.1"
                     /translation="MHRTIPPVSGYWRLTSCQGIDSRSQGAARLPWAAITLRAINLEQ
                     FSTKTPVGLRLSRKGDSYE"
     gene            1376..>1771
                     /locus_tag="PKI05_14315"
     CDS             1376..>1771
                     /locus_tag="PKI05_14315"
                     /inference="COORDINATES: protein motif:HMM:NF027657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4964 domain-containing protein"
                     /protein_id="HOA53424.1"
                     /translation="MTRLIASTTVLLFISLISGRQTGVWANDGPAGRPPAVPLVVHDP
                     YFSIWSFTDQLAADWPRHWTGRIHALCSLIRIDGRPYRLMGMAPADIPPLEQTSLEVT
                     PTRTIYLWRTQEVEVRLTFLTPVLPHDMAL"
BASE COUNT          376 a          519 c          487 g          389 t
ORIGIN      
        1 cgaatgagcc aatcggcaaa cacctcccgc gggtcccggt ctgccgggat tgtgacctta
       61 gtaccatcgg gaaagacacc cacgagcggc tcctgactga tatccgccgg ggtcggggtc
      121 acgatagggt cttccgattt ccgcggagct gtccccggcg cctctcgtcc gggaacaaaa
      181 tttgtcggca ccaagcgaag gccccaggga tcccagaaga cgacctgctc cttccactcg
      241 ctggtgggct tataggcgat ctgggagaag aagacggcca taccttctaa ccgttccttg
      301 ggccaatggt ccgtccgtac ccccataagc gttagtgcca ccgttgaggc aattcccgtg
      361 gggctgcggt cctgcaccgc acgataaaag ttcaccgctc catctcggaa attgctgccg
      421 ctggccgtga gcatctcccg cacaaattgg tcgtaaggtt tgttcgatgc cagcgacgcc
      481 catatatagc gatgaaaggc ccttgccccg tttggccaca agttcaccgg aatctccgct
      541 tttattcgca gcgcgtcgcc ccacttcata gaccagtagt cgagaaactc cttccgctcc
      601 atcagccggt caactagccg ggcacgcttg tcgggagtct gatcatccaa aaactcgcgg
      661 acctcctggg ccgtaggaat ggtccccgtc atatccagga acgcgcgccg aatgaagacg
      721 gcatccgagc ataatatcgg ttggacgccc agcgtttgca acttggcgaa gacgattcgg
      781 tccagctccc cgttgggctg ggctggtgtg ggactttcat ataggctgat tgggtccgcc
      841 gcaaatccca agccgcccca caaaataacc gcgcacaaga cactcgttac agttttattc
      901 ataagagtca cctttccgag aaagccgtaa gccgacggga gtcttcgtgc tgaattgctc
      961 gaggttaata gcccgcaacg tgatggccgc ccatggcaaa cgagcagccc cttgcgaccg
     1021 agaatcgatc ccttggcatg acgttaaccg ccaatatccg gataccggtg ggatcgtgcg
     1081 atgcattttg aactccccga gcgcggaggg taagccaagg cggggatatc tgtgcgattg
     1141 tggacattcg cacaatcatt ttcgattgtc agcatagcaa gcccgcgggg gcttgtcaac
     1201 ttgtggcctg gaattgtttt tcttgccaaa gcgagcctag ctttacgtat cttgccatac
     1261 caagtggggg caccaaactc cgcgcgggtg gtcaggaatc atttcaaagt ccataatggt
     1321 ggttcgcggc tctcgggtac ttttggacga agcagtgagg tgcaacatgt ccagtatgac
     1381 aaggctaatt gcttctacca ctgttctgct gttcattagc ctcattagcg gacggcagac
     1441 gggggtgtgg gctaacgatg gtccggcagg tcggccaccg gcagtgccgc tggttgtaca
     1501 cgacccatac tttagcattt ggtcatttac cgaccagctc gcggcggact ggccacgcca
     1561 ctggacggga agaatccacg ccttgtgcag cctcatccgc attgacggga ggccgtatcg
     1621 ccttatgggt atggcaccgg cggatattcc tcctttggaa caaacttcgc tagaggtcac
     1681 gccaacccgc acgatttatt tgtggcgaac ccaagaggtc gaagtccgtc taaccttttt
     1741 gacacccgtt ctgccgcacg atatggcact c
//
LOCUS       DAOPTR010000900         1767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_52480_length_1767_cov_4.016939, whole genome shotgun sequence.
ACCESSION   DAOPTR010000900 DAOPTR010000000
VERSION     DAOPTR010000900.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52480_length_1767_cov_4.016939"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..367
                     /locus_tag="PKI05_14320"
     tRNA            297..367
                     /locus_tag="PKI05_14320"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:329..331,aa:Gln,seq:ctg)
     gene            1148..1450
                     /locus_tag="PKI05_14325"
     CDS             1148..1450
                     /locus_tag="PKI05_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53425.1"
                     /translation="MIRYICDLCRREIDSTREVCYHVEIQISAAFEPVTEDEPDDRDH
                     LEEIHDLLESLNQLGDEAYESAHETLRFDLCPQCRRRFLSNPLGRDAGKLLGFSKN"
     gene            1586..>1767
                     /locus_tag="PKI05_14330"
     CDS             1586..>1767
                     /locus_tag="PKI05_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53426.1"
                     /translation="MVARRTSIPLGPLLGSWLAPSRFTKSRRRRKQPAAWLLLLIVVL
                     FVFFRVFVHQNPFDSST"
BASE COUNT          400 a          452 c          497 g          418 t
ORIGIN      
        1 ggccgtcgag cgagaaggct atcggatgct tggggccatc attgaatgcc aggacggttt
       61 tctctatttc atcaagttct acggtcccca ggcaaccgtc aaagaaaacg agttggcctt
      121 ccgggcgatg atcgaaagtt tgcaggccgt tggcccacag taagcagtgc ttgtcggcga
      181 tttttgtgcc atccctggag ccgtttttcc cggtaaattg ggcacccgat cttgacggga
      241 acgagacaat ggcatatggt gataacagaa cggcccatgt gattcgggcc ggttattggg
      301 gtatggtgta acggtagcac gactgactct ggatcagtta gtctaggttc gaatcctagt
      361 accccagcta ggcactattc aaaacgaatg ccagcggggc gcggcgattt ctgctctctt
      421 ggtcaggaaa ttagtgggcc gttatcttag gccctctcga cttgtgactt ctcgataaga
      481 aaccaagcca tctgaggcaa tgggcggccc tagcagcagg ttgctttctg cacgacgacc
      541 atgaggcggg ctgtacttgg cacgagtcga attccagttt ctccacgggt ttcaccgcgg
      601 agttccgcag aaaccaaaaa gctcaaggtc ttgtacttcg ccaatgagtt tagaaagcca
      661 agtcgtttta tcaagcgatc ggggccggcg cacatggtcg tagagaacgt atccccaaaa
      721 gtccccctct ccctccggga gagggttggg gtgagggcaa gcgaacccgg aatcgattga
      781 gaaaaccggc agcgattcga gtcaccctca cccccgcccc catctcccgc ggaacgggcc
      841 agagattatg ggataggctc agaagagtga ttgtcccgcg gtgaggcaag tgattggctg
      901 gtagaggtga gcgtgtagga ccagcggcaa ccgtgatcag aaactgggat tgagaacaaa
      961 tcccaaccac cttgtcaaga tggtttctgc tcgagatgtg cagaggtcct taacaaatca
     1021 aaaatcttgc aattgttctc ttgtttcatt cgtcctgatt ctattaggat gaagctgttg
     1081 gggatttggg ctcgactttg gtgacgtatg cttgattcac agtccctcac cacggggaga
     1141 gcaggcgatg atccgttaca tctgcgatct gtgtcggcga gagatcgatt caacccgcga
     1201 agtgtgttac cacgtggaga ttcagatttc ggcggccttt gagccagtca cggaagacga
     1261 gccggacgat cgcgatcact tggaagagat tcatgatctt ctcgaaagcc tcaaccaact
     1321 cggtgacgag gcctatgaat cggcccatga gacgttacgg ttcgatttgt gtccccagtg
     1381 tcggcggcga tttcttagca atccgctggg gcgcgatgcc ggaaagctcc tgggctttag
     1441 caagaactga cagcgtcccc cggtgatgaa gatcttacga accgctgccg ggtaacatga
     1501 actgctgccc gggaaaaggt gggatcggtc taaaaagccc atcctcaagg tttgtgtctt
     1561 tcaaacaggt ctggagacgg ggccaatggt tgctcgacga acctccatcc cattaggccc
     1621 acttcttggt tcgtggttag ccccgagtcg attcacaaag tctcgtcgaa gacgtaagca
     1681 acccgccgct tggttactgc tcctcattgt cgttctgttc gtgttctttc gggtgttcgt
     1741 ccaccaaaac ccattcgaca gtagcac
//
LOCUS       DAOPTR010000901         1760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_52772_length_1760_cov_3.812903, whole genome shotgun sequence.
ACCESSION   DAOPTR010000901 DAOPTR010000000
VERSION     DAOPTR010000901.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52772_length_1760_cov_3.812903"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..637
                     /locus_tag="PKI05_14335"
     CDS             125..637
                     /locus_tag="PKI05_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53427.1"
                     /translation="MVKTCSRRISQERHSRLERRFAVTTMEIIVAMFLLATLAMIASQ
                     IIATVVQTRVEQRKRAFALQEAGNAMEEAFLLSWQELKPGQSRQWTCSDTAAKILKNA
                     KVTLVTEATPDDNTRRLVVCVEWSSGGGREPQRIQLVAFRHRAARSEEAAAAREAKGT
                     EKGIAASEEK"
     gene            640..1179
                     /locus_tag="PKI05_14340"
     CDS             640..1179
                     /locus_tag="PKI05_14340"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOA53428.1"
                     /translation="MSNKRRGYTLIEMVAVAVLSTFILGAAVATTALVTGVSTSTSRE
                     IDFYHELARLANSFRSDAHRAREVSATGMEGVLKVPGDGAIIGEMKLADGRSVRYVVG
                     QSLMREVYRDGKLVARDGFELRRGQRVRVEKREKNGRVFVHLSVVGAPVFDKGDQLAA
                     GRVWEVQAVVGRDLLPVEP"
     gene            1216..1725
                     /locus_tag="PKI05_14345"
     CDS             1216..1725
                     /locus_tag="PKI05_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53429.1"
                     /translation="MQRTVKLDYQPLDNMWQRGLIYDVARRLQTRGVAALAVIVCLII
                     VASLGINAIVDLKASWDDFRQARTALQADWLLASAFDRVRSRLAHDPAYVGEVWIVPA
                     DQLDGRLSAEIRTAITRTPDSSGKVILKVVLELKANGQTRISLTRINQVEGVKQEAET
                     PSSSSETPR"
BASE COUNT          404 a          411 c          583 g          362 t
ORIGIN      
        1 cgtgagaatc gattgagaaa accggcagtg attcgagcca ccgttacccc cgacccctct
       61 cccgccgaac gggtgagggg agattagatt ggctcttagt atttgattga atgtgaagca
      121 accaatggtc aagacatgca gtcgaaggat cagccaagaa cgtcacagta ggcttgaacg
      181 tcgctttgcc gtgacgacca tggagatcat cgtggcgatg tttctcctgg cgaccttggc
      241 aatgattgcc agccaaatta tcgccacggt cgttcaaact cgggtcgagc agcgaaaacg
      301 ggcctttgcc ttgcaggagg caggaaatgc catggaagag gccttcctct tatcctggca
      361 agaactgaaa ccggggcaaa gccggcagtg gacgtgctcg gacacggccg ccaagatttt
      421 gaaaaacgcc aaagtgacgc tggtcacgga ggccacgccg gatgacaata cccggcggct
      481 tgtcgtgtgt gtcgaatggt catccggcgg tgggagggag ccgcaacgga ttcaacttgt
      541 ggcgtttcgg catcgcgctg cgagatcaga ggaggccgcg gcggcgcgtg aggcaaaggg
      601 gaccgaaaag ggcattgcgg caagtgagga gaaatgatcg tgagcaacaa acggcgggga
      661 tatactctca tcgagatggt agcggtggct gtgctctcga catttatttt gggtgcagcc
      721 gtggcgacga ccgccctcgt gacgggagtg agtaccagca catctcggga aattgatttt
      781 tatcacgagc tggcgcgact ggccaatagt tttcgaagtg atgcccatcg cgcaagggag
      841 gtcagcgcca caggtatgga gggcgtcctc aaagtccccg gtgacggcgc gattattggc
      901 gaaatgaagc tggcggatgg tcgaagcgtg cgttatgtgg tgggccaaag cctaatgcga
      961 gaggtttatc gagacggcaa attggttgcc cgggatggct ttgaactccg gcggggacaa
     1021 cgagttcgcg tggaaaaacg cgagaagaac ggccgggtgt ttgtccatct tagtgtcgtg
     1081 ggggcacctg tgttcgacaa gggtgaccaa ttggcagcag gacgtgtttg ggaggtgcag
     1141 gcagtcgttg gccgcgatct cttgccagtt gagccatagc acgtgatatg ggacgaagat
     1201 aaaagcaggt cagccatgca gcggacagta aagctagatt accagccgtt agataacatg
     1261 tggcagcggg ggttgattta cgacgttgcc cggcgtttgc aaacacgtgg cgtggcagcc
     1321 ctggccgtga ttgtatgtct gatcattgtg gcttcgctgg ggataaatgc gatcgtggac
     1381 ctcaaggcga gttgggatga ttttcgacaa gcgcggacag cacttcaggc cgattggctg
     1441 cttgcctcgg cgtttgatcg ggtgagaagt cgcttggccc atgatccggc ctacgtgggg
     1501 gaagtgtgga tagtcccggc ggatcaactc gacggacgct tgtcggccga gatcaggaca
     1561 gcaataactc ggacgcctga ctcgtccggc aaagttattt tgaaagtcgt gcttgagctt
     1621 aaggccaatg gtcaaacccg catcagtttg acgaggatca accaggtgga gggggtgaag
     1681 caagaggcgg aaacgccgag tagcagttcg gagacaccgc gttgaatcgt tttgcgcata
     1741 gcgaggagtt accccatgtg
//
LOCUS       DAOPTR010000902         1759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_52820_length_1759_cov_3.992371, whole genome shotgun sequence.
ACCESSION   DAOPTR010000902 DAOPTR010000000
VERSION     DAOPTR010000902.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52820_length_1759_cov_3.992371"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..585)
                     /locus_tag="PKI05_14350"
     CDS             complement(91..585)
                     /locus_tag="PKI05_14350"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOA53430.1"
                     /translation="MWPNPGGQRVYTVHYYGILGPYGTNPTTNQTYKCRNTTEAFGGE
                     CDQGVMWQFSSRMADITDGTSNTLLLGEISWSQMTKYRAWTRGKFQDSRGTLYLLSKY
                     LRFPINSKNESIWNGIAFGSFHPGGCQFVNVDGSARFVSETIDFGIYLALGSKDGGEA
                     LQSQ"
     gene            complement(479..970)
                     /locus_tag="PKI05_14355"
     CDS             complement(479..970)
                     /locus_tag="PKI05_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOA53431.1"
                     /translation="MKRQAFTLVELLVVIAIIGILIALLLPAVQAAREAARRSQCVNN
                     LRQLGLGFQNYHDVWGQFPLPGMIANQLGWTASILAFIEQKPIYDQMDWNAGNYMDAG
                     KIKHAANRIPVYLCPSGDQTHGICLRDLAQPRWPKGIHGSLLWHSRPIRDQSDDQSNV
                     QMP"
     gene            1551..>1759
                     /locus_tag="PKI05_14360"
     CDS             1551..>1759
                     /locus_tag="PKI05_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53432.1"
                     /translation="MKRSVWAALIIFPILLGVIFGGVLGWWSVKRRPWPGYPILAEAQ
                     PAHSGARSEAGATSSEPLPKIDVPE"
BASE COUNT          428 a          467 c          474 g          390 t
ORIGIN      
        1 aacaagaaca ggatagcgac tcgctgaaag gaaaccatca cgtcgtgccc tttgctgccg
       61 atatatcaaa gaagtttctg agtgccacgg tcattgcgac tggagcgcct ctcctccatc
      121 cttgctgccc aaagcaagat aaatcccgaa gtcgatcgtt tccgagacga atcgcgcgga
      181 accatcaacg ttgacgaact gacatcctcc gggatggaag ctgccgaagg cgataccatt
      241 ccagatcgat tcattcttgg aattgatggg gaaccgaaga tattttgata gcaagtagag
      301 ggttccccgg ctgtcctgga atttgccacg cgtccaagcg cggtactttg tcatttgcga
      361 ccaggaaatc tcgcccaaga ggagcgtatt tgaagttccg tcagtgatat cggccattcg
      421 gctggaaaat tgccacataa cgccctgatc acactcgcca ccaaaggctt cggtcgtgtt
      481 acggcatttg tacgtttgat tggtcgtcgg attggtcccg tatgggccga gaatgccata
      541 atagtgaacc gtgtataccc tttggccacc ggggttgggc cagatctcgg aggcatattc
      601 cgtgcgtttg atctcctgaa ggacacaaat acacgggaat ccgattggcc gcatgcttga
      661 tcttaccggc atccatgtag ttgcccgcgt tccagtccat ctggtcgtaa atcggctttt
      721 gttcaatgaa ggccaggatc gaggctgtcc accccagttg attggcaatc ataccgggca
      781 agggaaactg tccccacacg tcatggtagt tttgaaaacc aaggccaagc tgccttaggt
      841 tattaacgca ctgggaccgt cgggctgcct cgcgcgccgc ctggaccgcc ggcagcaaaa
      901 gcgcgatgag gatgccaata atcgcaataa caaccaatag ttctaccaac gtaaacgctt
      961 gacgcttcat gaaaaacatc ctcaggaatg caggatcgtt cgaaacccgg caaacaaaac
     1021 gacaaaaaca ctccttaaaa acactccctc cggcactgtt cgtagtcggt gaacgtcgct
     1081 ccgagagcag ggaaccactc cctcttatgc ccgacgggca cgatcaatgt gcctcacctg
     1141 cacagcgtga gcacacgcgg gaagggaata ccgccgggtg ggtgcgagtt agtgacaaaa
     1201 gtgtgggtca cctttcccct ttctcccagg cattcttcga tgataagtgc acctaaagga
     1261 gatgtcaaca aagagcgtga cccggtccgc cgcgggaatt gtcgaatgct tttggaaaaa
     1321 atactagttc gagggcggct ttcagtggaa gcagcatgct tattcggttg gcaccacaca
     1381 caacactggt cacaaaaggc gtgtcaggcc gtttcgcacc gagcccaaaa aggtatcggc
     1441 ccaaaacccc cttttagtgg ctgcgtatgt aaaatggtag aacatgaccg tggttttcca
     1501 gcggcaatga gcggcggaat gatacaacca ttgaagcgga gcatgacgcc atgaagcggt
     1561 ctgtttgggc tgcattgatc atttttccga tcttgttagg tgtcattttt ggcggagtgc
     1621 ttggctggtg gagtgttaag cggcggccgt ggcctggtta ccccatcttg gcggaggccc
     1681 agccagccca ttccggggcg aggagtgagg cgggagccac ttcgtctgag cccctcccca
     1741 agatcgacgt tccggaaga
//
LOCUS       DAOPTR010000903         1757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_52889_length_1757_cov_4.456522, whole genome shotgun sequence.
ACCESSION   DAOPTR010000903 DAOPTR010000000
VERSION     DAOPTR010000903.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52889_length_1757_cov_4.456522"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1449)
                     /locus_tag="PKI05_14365"
     CDS             complement(<1..1449)
                     /locus_tag="PKI05_14365"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOA53433.1"
                     /translation="MRTVRVLWPSVFAILAVLYGREVLATDWPLDNNAVLESWGFVNV
                     AARPFEADPSGQRDSTEAIQKAINYARERQLVCLFPPGEYLISDTIVCEQYRPLRTPT
                     RRSGDRNHPCVLMGMERDGRRPCLVLSPQSKGFDDPDHPKPVVYFWAAGNGEEAPLDQ
                     PQPNISMNQMIVGVDLRLGPNHPGAIGISHRAAQGSGIQDCTIDARGAFCGVEGGAGS
                     GGSHAGITVIGGRYGLDLRGTQPAATVTGVTLINQDESAVICSGRQATTIVGFRIRTK
                     GNGPVILARQSGIHQGQLNLVDGTVEAEGSTSAVLLDTMAAAYFNNVFTRGVEKIVQS
                     PVGELKAVDAKWCHIQEAAIAVPPAGFRGRQLAALGVKELTYTCGIWLDGQKWSGPIW
                     HRLSATEAAPDDLVSRHLWDKQFPRPWSPGVVNAKLLENPPRGDGVTDDTEALQKAIE
                     SHPALFIPKGEYRITRTLRLRPDSQLIGAHRCF"
BASE COUNT          402 a          533 c          461 g          361 t
ORIGIN      
        1 aaagcaacga tgcgcgccga tcaattggct gtcaggccgc aaacgcaaag ttctggtaat
       61 gcggtactcg cccttaggaa taaagagggc aggatggctc tcaatggcct tttgaagggc
      121 ctcggtatcg tcggtcactc cgtcaccccg aggaggattc tcaagcagtt tcgcattgac
      181 gacgcccggc gaccatgggc gtggaaattg cttatcccaa aggtgacggc tcaccaaatc
      241 gtctggagct gcctccgtgg ccgaaagcct gtgccaaata ggcccggacc acttctggcc
      301 atccaaccaa atcccacatg tgtaagtgag ttccttcacg ccaagtgccg ccaactgacg
      361 accgcgaaaa ccggcagggg gaacggcaat cgcggcctct tgaatgtggc accatttggc
      421 gtcaacagcc ttgagttctc ccaccgggga ttgaacgatt ttttccacac ctcgagtgaa
      481 cacattgttg aagtacgcgg cggccattgt gtcgagcagc accgcggacg tggacccttc
      541 agcctcaaca gttccgtcga ccaaattgag ttggccctga tgaattccgc tttgcctggc
      601 caggattacc ggaccgttgc cttttgtcct gattcgaaaa cccacgattg ttgtagcctg
      661 tcgtccggaa caaatcacgg cggattcatc ctgattaatc agggtcacac ccgtgaccgt
      721 tgcggcgggt tgcgttcccc gaaggtccaa accatatcgc cctccgatga ctgttatccc
      781 ggcatgactg ccgcccgagc cggcaccacc ttccacacca caaaaggccc cgcgggcatc
      841 gatcgtgcaa tcctggatgc ctgacccctg ggccgcccga tgcgaaatgc cgattgcccc
      901 agggtgattc ggccccagcc gcaggtccac accaacgatc atctgattca tcgaaatatt
      961 tggctggggt tggtcaagcg gggcctcttc accgttaccc gcggcccaaa aatagaccac
     1021 cggcttaggg tgatcaggat catcaaatcc cttgctttgt ggggaaagga ccaaacatgg
     1081 gcgacggccg tcccgttcca tccccatcaa aacacacggg tggttgcgat ctcccgaacg
     1141 cctcgtagga gtccgcaacg gccggtattg ctcacagaca atggtgtccg aaatcagata
     1201 ttcaccaggc ggaaagaggc acacaagctg tctctccctc gcataattga tggccttttg
     1261 aatcgcttca gtggaatcgc gctgaccgct aggatcggct tcgaaaggcc tcgcggcaac
     1321 attcacaaat ccccacgatt cgaggacggc attgttatca agcggccaat ccgtcgcgag
     1381 aacttctctg ccatacaaca cagcaagaat ggcaaaaaca cttggccaca gcacacggac
     1441 agtacgcata atacctcccg cagcattgaa ttgagactcg caaccaatgt ctctcagatt
     1501 gaacgtctgc cccattgtga accattaccc gcttcaggac aatcaccggc cactttgtgc
     1561 aacgatggcc cgggggcccg ccattgcgga aaacggcgtt gatcggcacg ccaagtaggc
     1621 acaaggttcg cccttaccgg cgaccggaac gcgtgtgccg atttagagac tatcccataa
     1681 tctcccttca cgtgttcggc gcgagagggg tcgggggtga gggtggctcg aatcgctgcc
     1741 ggttttctca atcgatt
//
LOCUS       DAOPTR010000904         1755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_52981_length_1755_cov_4.254118, whole genome shotgun sequence.
ACCESSION   DAOPTR010000904 DAOPTR010000000
VERSION     DAOPTR010000904.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52981_length_1755_cov_4.254118"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /gene="ruvX"
                     /locus_tag="PKI05_14370"
     CDS             <1..307
                     /gene="ruvX"
                     /locus_tag="PKI05_14370"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOA53434.1"
                     /translation="VVGLPVHCDGSESESSQAAREFGHWLEAVTGTPVVFMDERFSTQ
                     TADTWLRSAHLSHKRRRERLDKVSAQIILSAYLATYKPSSGPPQSWSPSPLDDGESV"
     gene            485..1378
                     /locus_tag="PKI05_14375"
     CDS             485..1378
                     /locus_tag="PKI05_14375"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOA53435.1"
                     /translation="MHKAVIVGCGYVGSYVGRLWLKKGYCVCAVTSSPERVAPLEAQG
                     FEVVVADVLDPTSLERLPGGDVILYSVGPTGHRDVPRWRLYKEGLDNILTRFRHTTSQ
                     IGLVSSTGVYGNHPEGWIDEQTPRNPQRDSARALAAAEDLLLESSWGDRAYIFRLGGV
                     YGPGRIPLVSQLVQGQPLPTSPDAMLNLIHVEDAATAIDLVISRGQPPCIVNVVDGFP
                     VSRRDFYNELSRLLQVASPRFAESLPAFETDQVSSAARRHSPVTKRVSNQLLVKGFGM
                     SLRYPDYRSGLAEIVRQGFSQ"
BASE COUNT          358 a          449 c          505 g          443 t
ORIGIN      
        1 cgttgtcgga ttgccggtac attgcgacgg gtcagaaagt gaaagttcgc aagccgcccg
       61 tgaattcgga cactggttgg aagcggttac cggaacgcct gtcgttttca tggacgagcg
      121 gttttccact cagacggcgg acacgtggtt gcgatccgct cacctcagcc acaaacggcg
      181 tcgagaacgc ctggataagg tctccgcgca gatcattctt tcggcatacc ttgcgaccta
      241 caagccatcg agcggcccac ctcaatcgtg gtctccttca ccgctggatg atggggaatc
      301 cgtatgacgt ttgctcaacg tcacgagaca tggagcgaaa taggccttcg gtggttcaaa
      361 cagcgtggcc ctgaacgacg agagaatcgc aattgttctg gacgaggcac gttctaccat
      421 gcgataccga cgttgccatt cctgtaaaaa cgtcaatgtt cgctcgttcg ttggttgaaa
      481 tgttatgcac aaagccgtaa ttgttgggtg tggttatgtt ggcagctacg taggacggct
      541 ttggctgaaa aaaggctatt gcgtgtgtgc ggtgacaagt tcccccgaga gggttgcgcc
      601 ccttgaggcc caaggctttg aggtagttgt cgcagacgtt ctcgatccaa cttcgctcga
      661 gagattgcca ggcggtgacg tgatccttta ctcggtcggg ccaacggggc accgcgacgt
      721 tccacgttgg aggctctaca aagagggctt agataacatc ctgaccaggt ttcgtcacac
      781 aacctctcag attggcttgg tcagctcaac gggcgtgtat ggcaatcatc ccgaagggtg
      841 gattgatgag cagacaccaa gaaatcccca aagggactct gcccgcgcgt tggctgctgc
      901 ggaagactta ctgctagaat cgtcgtgggg cgatcgggcc tacatttttc gacttggcgg
      961 ggtttatgga ccagggcgga ttccgctcgt ttcccaattg gttcagggac aacctttgcc
     1021 gacttcccct gatgccatgc taaacctcat ccatgtcgaa gatgctgcta ctgcgattga
     1081 tcttgtcatc agccgtggcc agcccccgtg catcgtcaat gtcgtggatg gcttcccggt
     1141 tagccgacgg gacttctaca acgaattgtc tcgattgcta caagttgcct ccccacggtt
     1201 tgctgagtcc cttcctgcct ttgaaacaga ccaggtctca agtgcggctc ggcgccatag
     1261 cccggtgacc aagcgggtct ccaaccagtt gttggtcaaa ggcttcggca tgtcgcttcg
     1321 ctatcccgac tatcgaagcg gactagcgga aatcgtgcgg caaggtttct cgcagtgaag
     1381 attttaaccg aaaacaccaa aactgaccgc ttttttgcgt gtaattccta actcgagtgc
     1441 ggctcgtcag tgattgccga agtgcgagtt gattttcctt tccaatcggg taccctatga
     1501 aagtcgcgag gttgtcaggg gtgggagcga tgattgacac gtgcggcgtt gttgtctgca
     1561 cgagtgtggg cgcgggtttg cctcttttgc ctcattgtta ggactgtaac ctggaggttt
     1621 cgatgaacaa aacgtgtaat atgctggcca ggctaatgat ttggttcctg tgcgtggcct
     1681 ttatttgtgc tgacgtgatt gcttctgtcc gcgataacgg gtatcgcggg atttggttca
     1741 cgttgggcca gtgga
//
LOCUS       DAOPTR010000905         1755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_52988_length_1755_cov_3.168235, whole genome shotgun sequence.
ACCESSION   DAOPTR010000905 DAOPTR010000000
VERSION     DAOPTR010000905.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52988_length_1755_cov_3.168235"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..>1755
                     /locus_tag="PKI05_14380"
     CDS             19..>1755
                     /locus_tag="PKI05_14380"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOA53436.1"
                     /translation="MISRFFLDRPVFAWVIAIMIMVLGGLAIYNLPISQYPDIAPPTI
                     RITAFYPGGSAETVENSVTQIIEQKLTGLDDMIYFSATSDSAGSCNIELTFAPGTDPD
                     LAWAKVQNKLQLAMASLPEMVQRMGITVRKATRNYLLIANLISEDGRLGNIDLRDYAQ
                     SNLEKVLARVPGVGEVEVFGSQYAMRIWINPEKLTAYQLTVEDVIRAVRAYNVEVSAG
                     QFGGAPAVPGQRLNVSIVVQNHLQTPDEFANIPIVVQPDGGIVRIRDVARVELGTDSY
                     ASNIQFNGMPSAGLAVRLEPGANALDTADRVKKRLAELSHYFPPGVRVVYSYDTTPFI
                     RVAIFEVVKTLFEAVGLVFLAMLLFLGNLRATLIPTIAVPVVLLGTFAVLGAAGFSIN
                     MLTMFAMVLAIGLLVDDAIVVVENVERIMREEGLPPREATIKSMGQITSALIGIGLVL
                     SAVFGPMAFFPGSTGIIYRQFSVTIISAMLLSVVVALILTPVLCASLLKPIAKGHEPG
                     ESAVWFLRWFFVGFDRIFFWIRDQYVRVVHHSIRKKLPYLVGYVSILVLIADLFLRLP
                     TGFIPQEDQGVLM"
BASE COUNT          324 a          476 c          502 g          453 t
ORIGIN      
        1 agccaacggg aggtaaccgt gatttcccga ttttttctcg atcgaccggt cttcgcctgg
       61 gtgatcgcca tcatgatcat ggtcctgggt gggttggcga tctacaacct cccgatttcg
      121 cagtatccgg atatcgctcc gcctaccatc cgaattacgg cgttttatcc aggcggctct
      181 gccgagactg tggaaaacag cgtgacgcag attatcgagc agaaactgac cggcctcgat
      241 gacatgatct atttttccgc cacgagcgac tcggcaggtt cttgcaacat tgagctgacg
      301 tttgccccgg ggaccgatcc cgatctggcc tgggctaaag ttcaaaacaa actccaactg
      361 gcgatggcca gtttgccgga gatggtccag cggatgggaa ttacggtccg caaggcaacc
      421 cgcaactacc ttttgattgc caatcttatc agcgaggacg ggcggctcgg aaacatcgac
      481 ctccgcgatt atgcccaatc caatttggaa aaggtcttgg cccgcgtgcc gggtgtgggc
      541 gaggtggagg tcttcggcag ccagtatgcc atgcggattt ggatcaatcc ggagaaattg
      601 accgcctacc agttgactgt ggaagacgtc attcgggccg tccgcgccta caatgtcgaa
      661 gtctccgcgg gacagttcgg gggcgccccc gccgttccgg gccaaaggct caatgtcagc
      721 atcgtggttc agaaccacct ccaaacgccg gatgagttcg ccaacattcc cattgtcgtc
      781 cagcccgatg gtggtatcgt gcggatccgc gatgtggcgc gggtggaatt gggtacggac
      841 tcctacgcta gcaatattca gtttaatgga atgccatcgg ccggcttggc cgtccgattg
      901 gaaccggggg ccaatgccct ggatacggcc gatcgcgtga agaaacgcct tgccgaattg
      961 agtcactatt tcccgcccgg tgtaagggtc gtctattcct acgacaccac gccttttatt
     1021 cgggtggcca tctttgaggt cgtcaagacg ctttttgaag cggttggcct ggtctttctg
     1081 gcgatgctgc tctttctggg taacttgcgg gcgacgctca tcccgacaat cgctgtgccc
     1141 gtggtcctct tgggaacgtt tgctgtcctc ggagccgccg gcttttccat caatatgttg
     1201 accatgtttg ccatggtctt ggcaatcggg ctgcttgtcg atgacgccat cgttgttgtg
     1261 gagaatgtgg aacgtatcat gagggaagag ggccttcccc cgcgagaggc caccatcaag
     1321 tccatgggtc aaattaccag tgccctgatt ggcattggtc tggtcctctc cgccgtcttt
     1381 gggccgatgg cctttttccc gggatcgacg ggaatcatct atcggcagtt ttcggtcacg
     1441 attatttcgg ccatgttgct ttctgtggtg gtggccctta ttctgacgcc ggtcctctgc
     1501 gcatcgcttc ttaagccgat tgccaaagga catgagccgg gcgagagtgc cgtctggttt
     1561 ttgcgatggt tcttcgtggg ttttgatcgc atcttcttct ggattcggga tcagtatgta
     1621 agggttgtcc accattcgat tcgcaaaaaa ttgccctatt tggttggtta tgtgtcgatt
     1681 ctggtgttga tcgccgacct cttcctgcgg ttgcccaccg gttttattcc ccaagaagac
     1741 cagggcgtac tgatg
//
LOCUS       DAOPTR010000906         1754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_53025_length_1754_cov_3.356092, whole genome shotgun sequence.
ACCESSION   DAOPTR010000906 DAOPTR010000000
VERSION     DAOPTR010000906.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53025_length_1754_cov_3.356092"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..880)
                     /locus_tag="PKI05_14385"
     CDS             complement(230..880)
                     /locus_tag="PKI05_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53437.1"
                     /translation="MTGWDWFLALVPVGIYLIVIGALNLARRPVVMTGMQDRVLLGLA
                     TIGLIVVGPVELFLPLPVYVAYGPTVWLIAIVLLTLVIGLLILAAPPKLVIFNASVHE
                     VRSLVSETAVELDPESRWAGDCLHMPNLRVQLFLHVSPGWRNATLSAIGNNQDYQGWH
                     RFGQALTKRLAMLEVRPNLRGLYLLLTGSVLLATCMISAFREPTILSQLLSRIISS"
     gene            1202..>1754
                     /locus_tag="PKI05_14390"
     CDS             1202..>1754
                     /locus_tag="PKI05_14390"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA53438.1"
                     /translation="MNTLTRRKFLKVPVHLAVGSAGIVLLKNSQSVWSYQANQKLNVA
                     LVGVSGRGAWFVQAIPQIGENVAALCDANERRAAPAFNQFPNVPKFRDFRQMFDKIGN
                     SLDAVVVAVPDHNHAVISNTAICLGKHVYSEKPLTHDVWEARVLRINAAKYKVATQMG
                     NQGTATHAFRRGVELIQTKALGNI"
BASE COUNT          451 a          496 c          447 g          360 t
ORIGIN      
        1 acctcgccca cctcaagtcc ccgctccgtc cgcacgatga ctcggtcacc gcgcttgtag
       61 cgatccccct ctcgggtgct cgtgaagaca ccgagaaacc gcatcgcgcc aaaacgaacg
      121 atgtattgcg tgtccatagt ggaaaattgc cgatccttgt ttcccatatc agggacaaca
      181 ccgtgtttct gccggcatct aaatacctca ccttgttgcc caggaatagt catgaggaaa
      241 tgatccgact tagtaactgt gacaaaatcg ttggctcacg aaatgcagag atcatgcacg
      301 tcgccaagag cacgctgcct gtcaacaata agtatagacc ccgtagattg ggcctaacct
      361 ccaacattgc caatcgtttg gttaacgcct ggccaaagcg gtgccatccc tggtaatcct
      421 gattgttgcc gatggcgctt aacgtggcat ttcgccaacc tggggagacg tgcaaaaaga
      481 gctgaacccg caaattgggc atgtgcagac aatcaccggc ccaacgggac tccggatcaa
      541 gctccacggc agtttctgaa accagcgatc gcacctcgtg aaccgatgca ttgaaaatca
      601 ccagctttgg gggagccgca agaatgagca accctatgac aagggtcaaa agtacgatcg
      661 caatcagcca gacggtggga ccatacgcaa cgtaaacggg caggggcaga aacagttcga
      721 ccggccccac cacaataagc ccaattgtgg caagccccaa aagtacccga tcttgcatcc
      781 cggtcatcac taccggtcgc cgcgccaggt ttaacgctcc gatgacgatg agatagatgc
      841 cgacgggcac aagggccaaa aaccaatccc atccggtcat tttcttcctc tttccctgca
      901 aacgtggcag cctaacgaac gtgagaacgc taggcgggcg actccctaca acgtcattgt
      961 agtggagcca tgcggacagc gtcgagactc agtaacacct caaaatgcca gaagagggcg
     1021 aaagtacgca ggatgtcacc ttcctccagg ggaggatgtc tcctcccgac ggaaataaat
     1081 cccgttctcc caactacggt aaggcttgat ctccagactg ttgcctgcca tcctccacgg
     1141 tgatacattg ttcgatgtcc ctgtgtccgt cagtcgcaac tatcgacgag ggaggctcac
     1201 cgtgaacacc ttgacgagaa gaaagttcct taaagttccc gttcacttgg ctgttggctc
     1261 ggcgggaatc gtgcttttaa agaattccca aagtgtgtgg tcctaccagg ccaaccaaaa
     1321 gctgaatgtt gcactggttg gggtttcggg cagaggggcg tggttcgttc aggccatccc
     1381 ccaaattggc gagaatgtgg ccgctctttg cgatgccaat gaacggcgag cggctcccgc
     1441 attcaatcaa tttccgaatg tgccgaagtt tcgcgacttt cgccaaatgt tcgacaaaat
     1501 tggcaacagc ctggacgccg tcgtggttgc cgttccagat cacaatcacg cagtgatttc
     1561 caatacggca atatgtctgg gcaagcacgt ctatagcgaa aagccgttga cccatgacgt
     1621 atgggaggcc cgggttctgc gaattaacgc ggccaaatat aaagtcgcca cgcaaatggg
     1681 aaatcaaggc acggcaacgc atgccttccg ccgaggcgta gagctaattc aaacgaaggc
     1741 gttaggaaat attg
//
LOCUS       DAOPTR010000907         1754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_53027_length_1754_cov_3.267805, whole genome shotgun sequence.
ACCESSION   DAOPTR010000907 DAOPTR010000000
VERSION     DAOPTR010000907.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53027_length_1754_cov_3.267805"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PKI05_14395"
     CDS             <1..763
                     /locus_tag="PKI05_14395"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOA53439.1"
                     /translation="KAQHSLLNGLLTGFAGDLVLIAVAMVFVMRDWSAGLLLALASVF
                     PIAVVFGAMGWLGIVVDVGTVMTPAVALGVTVDDAIHFMLWCRHGQERGMNRHESIMF
                     AYRDCAQAIYQSWGVIGLGLSAFALSSFSPTQRFGYLMLAMLTASSFGNLVLLPALLA
                     SPLANYFWRSGAKQAERKRSQLKTQKAPDLHPHVIPLEQPAFEEEPVGVCATSEGVIY
                     LETGGDGVTPSFPSERDCPQRWSGARKHRLPHKPR"
     gene            1012..>1754
                     /locus_tag="PKI05_14400"
     CDS             1012..>1754
                     /locus_tag="PKI05_14400"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HOA53440.1"
                     /translation="MAQKASTREIPYLAQRSRKGVITGVQVLGLGSYLPETRVRNEDL
                     VKLGCDPEWIIQRTGILERRHAPPEMATSDMAVEAGRRCLLSAQVNPKEVDLVVFGTY
                     TPDMTMPATASLVQEKLGLFAPAMDIQAACTSFMFALITGMQFVGTGCAKRPLVIGAD
                     CNSRVCNPNDPKTFPIFGDGAGAVVLGPGDKDQGLIAFSYGSDGAGFDLLYRPAGGTR
                     TRKDEPGQLVDDGYLVMEGRPVFKWAVRML"
BASE COUNT          341 a          456 c          533 g          424 t
ORIGIN      
        1 taaggcgcaa cactcgctgc taaatggcct cttgacaggc tttgccggcg acctcgtcct
       61 cattgccgtc gccatggtgt ttgtcatgcg ggattggtcg gctggcttgc tcctggccct
      121 ggccagcgtg ttccccattg ccgtggtttt cggggcaatg ggctggcttg gcatcgtggt
      181 ggatgttgga acggtgatga cacctgccgt agccctgggt gtcaccgttg acgacgctat
      241 ccactttatg ttgtggtgcc gtcacggtca ggagcgtggc atgaaccgcc acgaatcgat
      301 catgtttgcc tatcgtgatt gtgcacaggc aatttaccaa agttgggggg ttatcggttt
      361 ggggttgtca gcatttgctc tgagttcgtt ttcgccaacc cagcgcttcg gttacctcat
      421 gctcgcgatg ctaacggctt cgtcatttgg gaatcttgtc ctgctgccag ccctgttagc
      481 aagtccgctg gccaattact tctggcggag tggcgcgaag caggccgaaa gaaaacggag
      541 ccagctcaaa acgcaaaagg ctcccgacct acatccccac gttattcctt tggagcaacc
      601 agccttcgag gaagaacccg tgggcgtgtg tgccacctcg gaaggggtaa tttatctcga
      661 aacggggggt gacggtgtga cgccgagttt tccatcagag cgtgattgtc cccagcggtg
      721 gtcgggggca aggaagcacc ggctgcccca caagccgcgt tgacagcgcc tcaggctttt
      781 gtgcgattgg ctccttggtt cgtgccagtg atttgctgac cataatcgtc cggcaggttg
      841 ttttggggta ggggtcacta aaaaacggcc cgtcacctct tgcctctcat tgtgaaacga
      901 tttagtttaa ggggttgtcg tttcgttaat tcggagagca tccccccatc ttgggtcaag
      961 acagggtgac aattgggtta ctttcgagat ttgcaataaa ggttcctttt catggcgcaa
     1021 aaagcctcaa ctcgggagat tccttatctg gcacagcgtt ctcgtaaggg ggtcatcacc
     1081 ggagtccagg ttttagggtt gggaagctat ttacccgaga cacgggttcg caacgaggat
     1141 ctcgtcaagc ttggttgtga tccagaatgg attattcagc ggacgggaat cttggagcgc
     1201 cggcatgcgc cgcctgaaat ggcaaccagt gacatggccg ttgaggcagg taggcgatgt
     1261 ctgctcagtg cccaggtcaa tcccaaggaa gtcgatctcg ttgtcttcgg aacatacacg
     1321 cccgatatga cgatgccggc cacagcgagt ctcgtgcagg aaaaactggg actctttgcc
     1381 ccagccatgg atatccaggc tgcatgcacg agttttatgt ttgcactgat cacaggaatg
     1441 cagtttgtcg ggacgggctg cgccaagcgt cctctggtca tcggggccga ctgcaattca
     1501 cgggtctgca atcccaacga tcccaagacg tttcccatct tcggtgatgg ggcaggggcc
     1561 gttgtcctgg gaccgggcga caaggatcag gggttgattg cgttcagtta cgggtcggac
     1621 ggggccggat ttgatctcct ttatcgcccc gcagggggaa cccggacccg aaaagacgag
     1681 ccggggcagc tagtcgacga cggctatttg gtcatggaag gccgaccggt attcaagtgg
     1741 gccgtccgca tgct
//
LOCUS       DAOPTR010000908         1752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_53102_length_1752_cov_3.723630, whole genome shotgun sequence.
ACCESSION   DAOPTR010000908 DAOPTR010000000
VERSION     DAOPTR010000908.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53102_length_1752_cov_3.723630"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            263..955
                     /locus_tag="PKI05_14405"
     CDS             263..955
                     /locus_tag="PKI05_14405"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOA53441.1"
                     /translation="MMALGTDPQLAVIFDMDGVLIDSYWAHFESWRIVAAEEGLEITE
                     EQFSRTFGRTSREIIAALWGDKTPGEERIRELDERKEAAFRDLIRDNFPIMPGATELL
                     DALHREGFRVAIGSSGPPENVALTVEKLGRSNIFGAIITGKDVTRGKPDPQVFLLAAE
                     RLNVPPRWCAVVEDAPPGVEAAHRAGMKAVGFASTGRTREMLAQADLIVTSLFELSPQ
                     RLRDLILGGAST"
     gene            1309..>1752
                     /locus_tag="PKI05_14410"
     CDS             1309..>1752
                     /locus_tag="PKI05_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA53442.1"
                     /translation="MSEKQVSVATVETGTTKSAPEVVIETRTLSKVYRDFWGRTKVQA
                     LKALDLQIFRGEVFGLLGPNGSGKTTTMKLLLGLLFPTSGEVYVFGRPATDVSKNERI
                     GYLPEESYLYRFLNAEETLDFYARLFSMPRSVRRQRVADLIEMVGL"
BASE COUNT          365 a          484 c          516 g          387 t
ORIGIN      
        1 cgggttgccc ctttttcttg tcgccatgtt ccatcaaatg tggacgcaca tgcgtcatct
       61 cctcggtcct cgtaagcgtc ggtgaacctt gcttgctccc gaccagaacg gtgcggcccc
      121 aacgcgtcgg ccggggtcgc tttgttttct tgcctacttg cttcacaata gacggttcgt
      181 tcactgatat aggtggcaat ttccgtgggc aggattaaac cggctattga ttgacattgt
      241 ttcgcaggcg agggagttcc ccatgatggc actcggcacg gacccccagt tggccgtcat
      301 ttttgatatg gatggcgttt tgatcgactc ctattgggcc cattttgaga gttggcggat
      361 tgtcgcggcc gaagagggat tggaaatcac cgaggaacag ttttcccgca cgtttggccg
      421 cactagccgc gagatcatcg ccgccctctg gggtgacaag acccctggag aagagcggat
      481 tcgcgagctg gacgagcgaa aggaggctgc ctttcgggat ttgatccgcg ataactttcc
      541 catcatgccg ggcgcaacgg aactactcga tgccctccac agggagggat tccgagtggc
      601 gattggctcg tcaggtcccc cggaaaacgt tgccctgacg gtagaaaaac tcggtcggtc
      661 aaacatcttt ggggcaatta ttacgggcaa agatgtcacc cggggaaagc ccgatccgca
      721 ggtgttcctg ctggcagcgg agagactcaa cgtaccgccg cggtggtgcg ccgtggtaga
      781 agacgccccc ccaggggtcg aggccgccca tcgtgcgggg atgaaggccg tgggttttgc
      841 cagtaccggc cgaacgcgcg aaatgcttgc ccaagccgac ctcattgtca cgtccctgtt
      901 tgaactgtcg ccccagcgac tccgcgacct catccttggc ggggcatcga catagcggcc
      961 gtcaacaccg caccaattcg tctgacaccc gggaccaacg gtcctccggc aggggctacg
     1021 tacgccaatt aattgccgac tttttgccgt cggtttatag tggaagaaaa cgtcaggggc
     1081 aaaaaacgtc accaaagggc ccacgagccc gccagcgact gtttttcgac ctaagtactc
     1141 caccttaagg gggtattgga tactccattg gttcattcta gatcattcca ggagggaagc
     1201 ggcgatctga aaggcttgcg tacgaacctt tgaacagccg tccaaggtca tgcgtaaagc
     1261 gccacagtcg gcaagggaaa gatcttatcg gatcgaaggt tacaactcat gagcgagaag
     1321 caagtctctg tagccacagt tgagacggga acgaccaagt cggccccgga ggttgtgatc
     1381 gagacgagga cgctctccaa ggtctatcgg gacttttggg gacggactaa ggtccaggcc
     1441 ctgaaggccc ttgacttgca gatttttcgc ggtgaggtgt ttggcctatt gggacccaat
     1501 gggtcgggca agaccaccac gatgaaattg ctgctcggcc tgcttttccc tacatcgggg
     1561 gaggtttacg tttttggacg gccagccacc gacgtgtcca aaaacgaacg cattggctac
     1621 ctgcctgaag agtcgtacct ttaccgtttc ttaaatgctg aggagaccct ggacttttat
     1681 gcccggctct tcagcatgcc tcgatcggtg cggcggcaac gggtggcgga cctcatcgaa
     1741 atggtgggcc tg
//
LOCUS       DAOPTR010000909         1749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_53221_length_1749_cov_3.396694, whole genome shotgun sequence.
ACCESSION   DAOPTR010000909 DAOPTR010000000
VERSION     DAOPTR010000909.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53221_length_1749_cov_3.396694"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..1509
                     /locus_tag="PKI05_14415"
     CDS             238..1509
                     /locus_tag="PKI05_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53443.1"
                     /translation="MRNVILIMALPVFFLGMALGWSLQMIPDILARREVPSNSGAKKE
                     VAIAQGTSHNKSEATQPSPDKKPESERSEVPSSSSGGQSSDTAAPSSASGSTSLAPAN
                     GKSSNTSSGKPDAKKNGHQTKENPNQQEQTHPQKSEVTPNAPQPQEGSLEVNSQDGSD
                     SGQLDATGAPKWRIRSVSHSLSKWFKIGILEEAKDSEGVGEKEPASPPGSDIAEGLSK
                     SEDWDLLIIDGKYIHCGSGRLSELKKWSVTLKEEAQPEGTIPGGKALFPLVVQVIPPS
                     EELPLDSGRDVRATLSLKRLVVEMEFETKSGEKSSPQRFSELYESAPSGEILISEASG
                     AKDLILRNKSTTPKLPGELKCNAKALVKEGKIEIQIDDPQISSRDGQLCKMEIKQIEG
                     EVMIDIVLDELDRKPIRVIKQHLRFPFDNPG"
BASE COUNT          486 a          432 c          478 g          353 t
ORIGIN      
        1 gcgcgaaagg gaacattcat tagacccccg gctgcagcgg tcacggaatc gaatcgaatc
       61 gcacccgccg tatcatctcc ccccagcacc ccggagtctt ttccacccgt cccaccagct
      121 cacacggtac ggcccccgat catgcgcgcc ccaatccgac ctccggaacg catttacgac
      181 ggcgtgccca tcggtagcag ttttccggac ggattcgaga ccgagaaagg tcggagcatg
      241 cgcaacgtga ttctgatcat ggcgctgcct gtgttctttc tgggaatggc tttgggttgg
      301 tcgcttcaga tgattccaga cattctagcg cggcgggagg taccaagtaa ctcaggcgca
      361 aagaaagaag tcgctattgc tcagggtact tcacacaata aaagcgaagc gacacaacca
      421 tcgccggata agaagccgga gtcggagcgc tctgaggtgc cgtccagctc atcggggggg
      481 caatcttcag acactgcagc gccaagcagc gcgagcggat ctacgagctt agcgccagcc
      541 aacggcaaat cgtccaacac atcctctgga aaaccagatg ccaaaaagaa tggtcaccaa
      601 actaaagaga atcccaatca gcaggagcaa actcatccgc aaaaatcgga ggtcactccg
      661 aacgcaccgc aaccgcaaga aggaagtctg gaagttaact cgcaggatgg ctccgatagc
      721 gggcagttgg acgcaaccgg ggcccccaaa tggcgtatcc gctctgtgtc acattcgcta
      781 tcaaaatggt tcaagattgg catacttgaa gaggcaaaag actcggaagg agtgggggag
      841 aaggaacctg catctccccc cgggagcgat atagcggaag gcctatcaaa aagcgaggat
      901 tgggaccttt tgataatcga tgggaagtac atacactgtg gtagcggtcg cctctcagag
      961 ttgaagaaat ggtcggtaac cctgaaagaa gaggctcaac ctgaaggcac aatcccgggt
     1021 ggtaaagcgc tattcccttt agtcgtgcaa gtaataccgc caagcgagga actcccactt
     1081 gattccggac gtgatgtcag ggccacgtta agtctaaaga gactagtcgt cgagatggaa
     1141 tttgagacaa aaagcggtga gaaatcaagt ccacaacggt tttcggaact ctacgagtca
     1201 gctccttcag gggagatact catttctgaa gcgagtgggg ctaaagactt gattttgagg
     1261 aacaagtcga caactcctaa gcttccgggc gaattgaaat gcaacgccaa ggccctcgtc
     1321 aaggaaggaa agatcgagat acaaatagac gatccccaaa tctcttcaag ggacgggcaa
     1381 ttatgcaaga tggaaataaa acagatagaa ggcgaagtca tgatagatat tgttctggat
     1441 gaacttgaca ggaaaccgat tcgtgtcatc aagcaacacc ttaggtttcc ctttgataac
     1501 cccggttagg gcgctgattt tcgggaaaat gccccagagc gatgagaatt gccgtttatg
     1561 cttggccgct tcgaaagtgt gttggctatg agtaagaaac cttgggttgg gacaaatgtc
     1621 agcttcgaag cagcgtgatc gatcggaagt gtgcgtggaa tggttaatcg agacacttcg
     1681 ttctctctaa ggacgctcag cctacggtaa tgtggacaca gtgtgagttt agcgagagtg
     1741 gtcgtattt
//
LOCUS       DAOPTR010000910         1747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_53316_length_1747_cov_3.117612, whole genome shotgun sequence.
ACCESSION   DAOPTR010000910 DAOPTR010000000
VERSION     DAOPTR010000910.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53316_length_1747_cov_3.117612"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /gene="metG"
                     /locus_tag="PKI05_14420"
     CDS             <1..1097
                     /gene="metG"
                     /locus_tag="PKI05_14420"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011120499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HOA53444.1"
                     /translation="VNGEKMSKSKGTFIKAETYLRHLDPSYLRYYYAAKLSSRVEDID
                     LNLEEFVSKVNTDLVGNIVNLASRTARFLEGQNLSPTYPDDSGLFAQAAADGQAIAEA
                     YEKCDFGKAMRLILAAGDRANQFIERAAPWNLKKDPAKQSQLRDVCTIGLNLFRQLVI
                     YLSPVLPRLAAQTGKLLNDPVSSWDQSQVPLVGNRVAPFEHMMTRVPKEKLAAVVEES
                     REVEVASGGGEGNRPAEGVSPETADTDETLQREPLLTSTVTIDDFAKIDLRVARVLAA
                     EEVPGADKLLKLTLSLGGNHTRTVFAGIKKAYKPGDLVGRLVILVANLAPRKMRFGTS
                     EGMILAAGWGDDQVFLLAPDQGAKPGHRVH"
     gene            complement(1178..1516)
                     /locus_tag="PKI05_14425"
     CDS             complement(1178..1516)
                     /locus_tag="PKI05_14425"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53445.1"
                     /translation="MPSVDEMYDEAIALQQQGDLPGALSKLQEIIQIDPNYALAYAAL
                     SVFYSKMEEHEKAVENARKVCELEPEDPFSFVALSLICQKAGLLAEAEEAMWQARQAQ
                     VAAIQKRYSS"
BASE COUNT          392 a          476 c          489 g          390 t
ORIGIN      
        1 cggtcaatgg cgagaagatg tcgaaatcca aggggacgtt tatcaaagcg gagacctatc
       61 tgcgccatct cgatccgtct tatctccggt actattatgc ggccaagctc agctcgcggg
      121 ttgaagacat cgacctgaac ctcgaagagt tcgtgtcgaa ggtaaacact gatctggtgg
      181 gaaacatcgt caacctggcc agtcggacgg cccgcttctt agaagggcag aatctctctc
      241 ccacgtatcc cgatgatagc ggactgtttg cccaggccgc cgcagacggc caggctattg
      301 ccgaggccta cgagaagtgt gactttggaa aggctatgcg gcttatcctg gccgccgggg
      361 atcgtgcgaa ccaattcatc gaacgagcgg caccgtggaa cttgaagaaa gatcccgcca
      421 agcaatcaca attgcgggat gtctgtacaa ttggtctcaa tctctttcgg caattagtca
      481 tttatctgtc cccagtgtta ccacggctag ctgcgcaaac gggtaagctc ctcaatgatc
      541 cggtttcttc ctgggatcaa tcgcaggtac cgttggtggg caatcgcgtg gccccgttcg
      601 agcatatgat gacgcgagtg ccaaaggaaa aactggccgc cgtggtggaa gaaagccgcg
      661 aggtggaagt cgcgtctggg ggcggcgaag gtaatcgccc ggcggagggt gtttcgccag
      721 agactgccga caccgatgag acgttgcaaa gggaaccgct tttgacctcc acggtcacga
      781 tcgatgactt tgccaagatt gacctgcggg ttgcccgcgt ccttgcggct gaagaggtac
      841 ccggtgccga caagctgctg aagttgacac taagtctggg tggcaaccac acccgcaccg
      901 tcttcgccgg gattaaaaaa gcatacaaac ccggcgattt agtaggacgc cttgtgatcc
      961 tcgttgccaa tcttgcgcca cgtaaaatgc ggttcggaac gagcgaaggg atgatccttg
     1021 ccgccggctg gggggacgac caggtatttc tccttgcccc tgaccaggga gcgaaaccgg
     1081 gtcaccgggt ccattgaagc ccatccattt acgcccagtt ccaatagaaa tcacgccaga
     1141 taaacaaggg ccctggctgc gcgcctacga cggatgctca ggaagaatac cgcttttgga
     1201 tggcggccac ctgggcctgt cgggcctgcc acatggcctc ttctgcttcg gccaggagac
     1261 ccgccttctg acatataaga cttaaggcaa caaagctgaa tgggtcttct ggctcgagtt
     1321 cgcacacttt gcgggcattt tctacggcct tctcgtgttc ctccatcttg ctgtagaaaa
     1381 cgctcaaggc cgcataggcg agagcataat tcgggtcgat ttggataatc tcctggagtt
     1441 tgctcaaagc acctggaagg tctccctgct gctggagggc aatcgcctca tcgtacattt
     1501 catcgacaga aggcatggtt tgttctccta taaggtcaat tcactgtggt tggtgttgtc
     1561 tttcctggtt gttgcgcccg ccaaaggggc gaaatcatca cattaccttg cgggcaattc
     1621 gagggaaaac ccgaatgcat aagtttacgc cacccaatgc cgcttggcta gggctggaat
     1681 ctttctcaaa gttgttaaca ccgtaattcg cacccttcgg ggaaaaacat ctggtaccgc
     1741 ctggccg
//
LOCUS       DAOPTR010000911         1746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_53345_length_1746_cov_3.933176, whole genome shotgun sequence.
ACCESSION   DAOPTR010000911 DAOPTR010000000
VERSION     DAOPTR010000911.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53345_length_1746_cov_3.933176"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PKI05_14430"
     CDS             complement(<1..268)
                     /locus_tag="PKI05_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOA53446.1"
                     /translation="MTVRTRFAPSPTGYLHIGGVRTALFCWLFARRHGGQFILRIDDT
                     DQQRNVEEALRPILDGLRWLGIDWDEGPEVGGPCQPYFQSQRLPI"
     gene            complement(650..802)
                     /locus_tag="PKI05_14435"
     CDS             complement(650..802)
                     /locus_tag="PKI05_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53447.1"
                     /translation="MTLGGWIVMLLSVGGATGLLAWCLYKVLTTPGSEEHLGQVEIHT
                     PDMDQD"
     gene            complement(799..>1746)
                     /locus_tag="PKI05_14440"
     CDS             complement(799..>1746)
                     /locus_tag="PKI05_14440"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53448.1"
                     /translation="NVLSGLGYMWNPVKPGMTFWQALADPEMWMKAASQIFFSLSVGF
                     GIIITYASYVKKDDDIALSSLTAAAGNEFCEVVLGGLMVVPAAFVFLGPQIGQQLGNP
                     FALGFMALPSVFNQMPVGWLFGCLFFFLLFLAAITSSLSMLQPAVALLEEGLGLNRRA
                     SVAVLSFITAIGSAMVVYFSKDFVALDTLDFWVATFAIYLLATFQTILFGWVLGVEKG
                     MEELQRGAAIRIPAIFKPIIKYVTPLYLLAVFGLWLYKNFFALPKGQTGRIQQVFSDP
                     VVGGVVLFILAVAILFAFLIAQSVRNWRRIEAVQEEVII"
BASE COUNT          465 a          478 c          461 g          342 t
ORIGIN      
        1 aaatggggag ccgctgcgac tgaaaatagg gttgacatgg gccaccgacc tctggtcctt
       61 cgtcccaatc gataccgagc caacggagcc cgtccaggat tggccgcaac gcctcctcga
      121 cgttccgttg ttgatccgta tcgtcaattc gcaggataaa ctgtccccca tggcgtcggg
      181 caaatagcca gcaaaacagg gcagtcctca ctccgccaat atggagataa cccgtgggac
      241 tcggtgcaaa acgtgtacga accgtcataa gaacgtccga tgcgagatga tttgaataca
      301 ctccatcgac ccatcaacca acggttttgg tccaatcctt tctccctggg gagatgggac
      361 caccttagcg gtggtcagcg gggccatgga aaaccggtga aagaaattga gtatatcggc
      421 cgacgggttt tttggctacc agaaacggct tcgaccgatt ggactaaggc gtcataagaa
      481 gaagtatcgc tgacgttttg tacgcaccga tcgttggaat caaccacggg tagctacaag
      541 ggccatcctt accacatcgg tgtaagtttt tcgagtagta acagggccac taggccttct
      601 acctacccag tgacactttg aagagaagcg cttgacatgc agtcatcatt cagtcttgat
      661 ccatatcggg ggtgtggatt tcgacctgac caaggtgttc ttcactgcca ggtgtggtga
      721 gcaccttata aaggcaccac gccaacaggc ccgttgcccc tcccaccgat agaagcatca
      781 caatccagcc gccaagtgtc atatgatcac ctcctcctga acggcttcga tgcgccgcca
      841 attcctcacc gactgggcga tgaggaacgc gaaaaggatg gcaacagcga ggatgaaaag
      901 gacgacccca ccgaccacgg gatcactgaa aacctgttgg atgcggcctg tttgtccctt
      961 cggcagggcg aagaagtttt tgtacaacca caatccaaag accgccagaa gataaagggg
     1021 cgtcacatac ttgatgatgg gcttgaagat agccgggatg cggatggccg caccgcgctg
     1081 aagttcttcc atcccttttt ccacgcccag cacccagccg aaaagaatgg tctgaaatgt
     1141 cgcgagcaag taaattgcaa acgtggcgac ccagaagtcc agcgtatcca aggcgacgaa
     1201 atcctttgaa aaatagacaa ccattgccga gcctattgcc gtgatgaagc tcagcaccgc
     1261 aaccgaggcc cgacggttta gccccagccc ctcttcgagg agggcaaccg ccggttgcaa
     1321 catggaaagg gaactggtga tcgcagcgag gaatagcaag aagaagaaca gacatccaaa
     1381 gagccagccc accggcatct gattgaaaac gctggggagt gccatgaatc cgagggcaaa
     1441 cgggttaccc aattgttggc cgatttgggg accgagaaag acgaacgcgg cgggaacaac
     1501 cataagccca cctaggacca cttcgcagaa ctcgtttccc gctgcggcag taaggctcga
     1561 cagggcaata tcgtcatctt ttttgacata acttgcataa gtgatgatga tcccgaaacc
     1621 cacggagagg gagaagaaaa tttgactggc cgccttcatc cacatttcgg gatcggcaag
     1681 tgcctgccag aacgtcatcc cgggtttaac cgggttccac atataaccaa ggccgctgag
     1741 gacatt
//
LOCUS       DAOPTR010000912         1742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_53514_length_1742_cov_3.101956, whole genome shotgun sequence.
ACCESSION   DAOPTR010000912 DAOPTR010000000
VERSION     DAOPTR010000912.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53514_length_1742_cov_3.101956"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1422)
                     /gene="hcp"
                     /locus_tag="PKI05_14445"
                     /gene_synonym="priS"
     CDS             complement(130..1422)
                     /gene="hcp"
                     /locus_tag="PKI05_14445"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF003658.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOA53449.1"
                     /translation="MFCNQCEQTANCTGCVISPGVCGKNEDVQSLQETLLYGLKGMAA
                     YAHHARRLGMVDEAVDSFMEEALFATMTNVNFDLDSLFELVLECGRHNLRVMEMLDEG
                     HVKRYGQPSPSLVKEGTQAGPGILITGHDLRDLEELLAQCEGTPVKVYTHGEMLPAHM
                     YPKLREHPNLAGHYGGAWQKQRREFDEFSGPIVATTNCILIPKESYKDRVFTTRVTAV
                     PGGVRLKDGDFSPVIKKALECPPLPDNIVRTSTVGFHHNVILGLADTIVQAVKNGEIS
                     RFFVIGGCDGAEVGRNYFSDFARLTPPNSFILTLGCGKYRIREYDYGTLLGLPRLLDM
                     GQCNNAYGAIKVALGLAEAFGCSVNDLPLTLVVSWFEQKAVAVLLTLLYLGVKGITIG
                     PKPPAFITPKVFKILQEKYDLRLIGSSAEEDLKRVLAA"
BASE COUNT          405 a          502 c          428 g          407 t
ORIGIN      
        1 atcacagcag attctaactc aatgagactt gtcactcaac tgcctattta ggcgaaggcc
       61 gatgttgtac cagcttgctg agttccttgg cacagcggtg tgctgtaggc caatgacgcc
      121 agccaatctt tatgctgcca agactcgctt gaggtcctct tccgcactgc ttccaatgag
      181 ccgaagatcg tacttctctt gcagaatctt gaacaccttc ggtgtgataa aggccggcgg
      241 cttcgggccg atggtaattc ccttaacgcc caggtacagc agggtcaaaa ggacggccac
      301 agccttttgc tcaaaccacg agacgaccaa agtcagcggt aggtcgttca cggaacaacc
      361 gaaggcctcc gcaagcccca gtgcgacctt aatggctcca taggcgttgt tgcattgtcc
      421 catatccagc agacggggca acccgagcaa cgtgccgtag tcatattccc gaatccgata
      481 ctttccgcaa cccaacgtca gaatgaacga gtttggcggt gttagccttg caaagtcgct
      541 aaagtagttt cgccccactt cagcaccatc gcacccgcca atgacgaaga agcggctgat
      601 ttcaccattt ttcacggcct gcacgatcgt atcagctaat ccgaggatga cgttgtgatg
      661 gaagccgacc gtgcttgtgc ggacaatgtt gtccggaagg ggcgggcact cgagagcctt
      721 cttgatcacg ggcgaaaaat cgccatcctt aagccggact ccccctggaa cggctgtgac
      781 ccgcgtagtg aaaacccgat ctttgtaact ctccttggga atcaggatgc agttagttgt
      841 cgccacaatg ggaccagaaa actcgtcaaa ttcgcgacgc tgtttctgcc aggccccgcc
      901 gtaatgacct gccaagttag ggtgctcgcg caacttgggg tacatatggg cagggagcat
      961 ttccccgtga gtatagacct tcaccggcgt accctcgcat tgggcaagca gttcctccag
     1021 atcacgcagg tcgtggcccg taatgaggat ccccggccct gcctgggtcc cttctttgac
     1081 caggctaggc gacggttgcc cataccgctt gacgtgaccc tcatcgagca tttccatgac
     1141 tcgcaaatta tggcggccac actccagcac aagctcaaac aggctgtcca ggtcaaaatt
     1201 aacgtttgtc atggtggcaa acagggcctc ctccatgaag ctgtccacag cttcatcgac
     1261 cattcctaat cgccgcgcat gatgggcgta agccgccatg cctttcaacc catacagcag
     1321 ggtctcttga agcgactgta cgtcctcgtt tttgccacag acacctgggc taatgacaca
     1381 acctgtgcaa ttggctgtct gttcgcactg gttacaaaac attgcattct ctccttttca
     1441 aagggctttc caaaaactgc ctctcgggct tcgtaacatc gatgctccga ccactagtga
     1501 ccgtgttcgt cctgatacag aatcgtgact tgataacggt aagagtctta actcagtctc
     1561 gtcttgcgac ttcacacgtt cgggtaagcc actccgaatc agtcacgtca tcgatcggtc
     1621 aacactataa taccgtctgg ggcgcgaaag gtttttgacg tagatcaagt tttcccaaaa
     1681 attgcccacg aggaacggta tggaactgac ggtgttagat atcctgcaaa gttgcccgct
     1741 ct
//
LOCUS       DAOPTR010000913         1740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_53591_length_1740_cov_3.532344, whole genome shotgun sequence.
ACCESSION   DAOPTR010000913 DAOPTR010000000
VERSION     DAOPTR010000913.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53591_length_1740_cov_3.532344"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PKI05_14450"
     CDS             <1..447
                     /locus_tag="PKI05_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOA53450.1"
                     /translation="HQVANGPEAARDVWELVEASPDPATLAIFTRPDQQWSIAELTPA
                     GREKMDDIATDHTPEWRALGVAVLHRLVVEHLLKLEPPLDIDYVHSIEEVILGLQTSN
                     YPMAVLVKPASVKDIRDVSLQGDRMPAKSTYFYPKLCSGLVINPLE"
     gene            1009..>1740
                     /locus_tag="PKI05_14455"
     CDS             1009..>1740
                     /locus_tag="PKI05_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008666690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOA53451.1"
                     /translation="MPRVICIANQKGGVGKTTTAINLAACLAKAGRRTLLVDLDPQCN
                     ATSGLGCQPLSHHPLVVGTPLSHAILTTSVKDLEILPGSRCFRDIESLVRDQGVYASM
                     LRNHLQTGMAGYDYVLIDCPPSVGQLTRTALAASNEVLMPIQCEYFAMEGLAQMIEVI
                     RRVMVERDRQLQFGGILLTMYDPRLELTLEVEEEVREFFGDIVFFTVIPRDVVCAEAP
                     SHGLPVIDYAPRARSTRAYVELCMEV"
BASE COUNT          359 a          499 c          490 g          392 t
ORIGIN      
        1 caccaagtgg caaatggtcc ggaggccgcg cgggatgtct gggagttggt agaggcttcc
       61 cccgaccccg caaccctggc gatctttact cgtcccgatc aacagtggtc catcgcggag
      121 ctgacgcccg ccgggcgaga gaagatggac gacatcgcca ccgaccacac accggagtgg
      181 cgtgctttgg gcgtagccgt tctgcatcgg ctcgtcgtcg agcacctcct caagctggag
      241 ccccccttgg acatcgacta cgttcacagt attgaggagg tcattctggg cttgcaaaca
      301 agtaactatc cgatggccgt cctggtcaag ccggcttctg tgaaggacat tcgcgacgtt
      361 agcctacaag gagaccggat gccggccaag agcacctact tttatcccaa actgtgctca
      421 gggctggtaa ttaacccgct tgagtaagcc ggcaattggt ctcttcagag ggcgtgataa
      481 gtgaggcaaa agcccaacgc cggtcctggt tttcgctccg cctgagtgaa tttcccggtt
      541 tcacgtggaa caggatctaa ttcctcggta gcttggagcc tatcccataa gctcccttca
      601 cgcgtttggt gggcgagggg tcgggggtga gggtggctcg aatcgctggc ggtcttctca
      661 atcgattccg acgttcgctt gccctcaccc taaccctctc ccaaagggag aggggagttt
      721 ttgggatggg cccttaatgg gttcttactg gtatccggat gcagaactgg atggcgtctg
      781 cagccccttc cagcgattac cagaaagctc acacgtagct attgacaccc tttgctggtt
      841 ccactgccga ttgccgttcc ctttttccgc caggattgat aaaatctgcc caaaagagtg
      901 atacgtcgtt ccacgtgaaa caggcagttt ggaaaactcg cagcgatagg atcgcttgtt
      961 gcaacggatg gcaaacgccc ttcatggggc cggcacggag gcgcgggcat gccgcgggtc
     1021 atttgcattg ccaatcagaa aggaggcgtg ggaaaaacaa cgaccgcgat caatttggct
     1081 gcttgtttgg ccaaggcagg tcgtcgaacc ctcctcgtcg atctcgaccc gcagtgcaat
     1141 gccacatccg gtttagggtg tcagccgctt tcccatcatc ccctcgtggt gggaacgcca
     1201 cttagccatg ctatcctaac cacaagcgtc aaagacttag aaatcctgcc gggcagccgt
     1261 tgttttcgag acatcgagtc tcttgtccgc gatcaagggg tctatgcctc gatgcttcgt
     1321 aatcacctgc agacaggaat ggccggctat gattacgtcc tcatcgattg tcccccgtcc
     1381 gtcggacaac tgacgcgcac cgcgttggca gcatccaacg aagtgctcat gcccattcaa
     1441 tgcgaatact tcgccatgga aggcctcgct caaatgattg aggtcatccg gcgcgttatg
     1501 gtcgaacgcg atcgtcaact ccagtttggg ggcatcctgc tgaccatgta cgatccgcgg
     1561 ttggagttga ctctggaggt cgaggaagag gtccgcgaat tcttcggaga catcgtcttt
     1621 ttcacggtga ttccgcgcga cgtcgtgtgc gcggaagcac ccagccatgg tttacccgtc
     1681 atcgattacg caccaagggc acgaagcacc cgtgcctatg tcgaactttg catggaggtc
//
LOCUS       DAOPTR010000914         1739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_53649_length_1739_cov_3.649644, whole genome shotgun sequence.
ACCESSION   DAOPTR010000914 DAOPTR010000000
VERSION     DAOPTR010000914.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53649_length_1739_cov_3.649644"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PKI05_14460"
     CDS             <1..424
                     /locus_tag="PKI05_14460"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOA53452.1"
                     /translation="ATVTFLRNAFRSETIADFAGLIRTSPGVVVCMSLALFSLVGLPP
                     LAGFAGKFAIFSSLVFGGLWSLFVIAAINTVLSLFYYVRIIKTMVFDVPVRDPLPQVR
                     LASPKGIFLAVLTVPVIVLGILWQPVFMLACRAAGYPW"
     gene            446..907
                     /locus_tag="PKI05_14465"
     CDS             446..907
                     /locus_tag="PKI05_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53453.1"
                     /translation="MTRKHGYMLTPLAMLNTLLNRLSRSLACYICQARPFVSYRDENV
                     WQVIEEIASRHRELSHRLSEEIIRLKGVPDAGSFPPTYTRFNDLAVDYIAPKIAQELE
                     ETLTIIQKAKSEYHGHGELDNLLNQLAEEVQAEIEALQRELHEAHPQECLK"
     gene            953..>1739
                     /locus_tag="PKI05_14470"
     CDS             953..>1739
                     /locus_tag="PKI05_14470"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOA53454.1"
                     /translation="MWFDTHAHLYLLLDLAESGLPDRVTIERELSPILQKAKEEGVRG
                     IICPGIDRSTSELAITIADLFPSFVWAAVGIHPNSASQGTEEDWDVITQLATHPKVVA
                     IGETGLDWFRNYTPRDQQVTWFRRHIELSRHVNKPLIVHCRDAEDDMTAILHEVAVSG
                     KCIGVMHACAARWETVEQWLDFGMFVSFAGSVTFSNKKHLFVRETACHVPLERLLIET
                     DCPFLVPEPLRGRASVCLPSHIALTGTFLAKLRNVPVPDLAQVT"
BASE COUNT          391 a          439 c          449 g          460 t
ORIGIN      
        1 tgccacggta acgtttctga gaaatgcctt tcgctctgag acaatcgcag actttgccgg
       61 attgattagg acctctccgg gtgttgtcgt ctgcatgagc ctggccctat ttagccttgt
      121 ggggttgcca ccacttgccg gctttgccgg aaagtttgcc atcttttctt cgttggtttt
      181 cggcgggctg tggtcgcttt ttgtcattgc agccatcaat acagtgctca gcctttttta
      241 ttacgtgcgt attattaaga cgatggtgtt tgacgtgccg gttcgcgatc ctctgccaca
      301 agttcgactg gcctcaccga aagggatatt cctggcagtc ttgacggttc cggtaatagt
      361 gttgggcata ctgtggcagc ccgtatttat gttggcgtgc cgcgctgcag gttatccttg
      421 gtaattgaat cggtcgaatt gcattttgac acgaaaacac ggttatatgc ttactccctt
      481 ggcaatgctc aatactctgt taaatcggct atcccggtcg ctagcctgct acatttgcca
      541 ggcacgacct ttcgtctcgt accgggatga aaatgtttgg caagtgatcg aggaaatcgc
      601 gtcacgacac cgcgaactgt cccatcgttt aagtgaagaa attatccgct taaaaggggt
      661 tcccgatgca ggatcgttcc caccaactta tacccgattt aacgatctgg ccgttgacta
      721 catcgctcca aaaattgcgc aagagcttga ggaaactctt acgatcatcc agaaggccaa
      781 gtccgagtat cacggccacg gggaactcga taacttgctt aatcaattgg ccgaagaagt
      841 gcaggcggaa atcgaggccc tccaacggga attgcatgaa gcccaccctc aagagtgcct
      901 taagtgatca tgttttcgcc gattaaatga ttcttgcgta cagaaggcac ccatgtggtt
      961 cgacacgcac gcccaccttt atctcctgct cgacctcgcc gaaagcggat tgcctgaccg
     1021 tgtcaccatt gaacgagaac tatcgccgat cttgcagaaa gcgaaagagg aaggcgtccg
     1081 cggcatcatt tgtccgggca tcgatcgctc aacgagtgaa ttggcaatca cgattgccga
     1141 tctatttccg agttttgttt gggcggctgt gggaattcat cctaactcgg cgtcgcaggg
     1201 caccgaggaa gattgggatg ttatcactca acttgcaacg catcctaagg ttgttgccat
     1261 tggggaaacg ggcctggatt ggtttcgcaa ctacacgccc cgggatcaac aggtgacatg
     1321 gtttcgacga catatcgaac tgagtcgcca tgtcaacaaa ccgcttatcg ttcattgtcg
     1381 tgacgccgaa gacgacatga cagcgattct tcatgaagtg gccgtttcag gaaagtgtat
     1441 cggggttatg cacgcctgtg cggcacggtg ggaaaccgtt gaacagtggc tagattttgg
     1501 gatgtttgtg agcttcgcag gatcagtgac ctttagcaat aagaagcacc ttttcgtccg
     1561 cgaaacagca tgccatgtcc cgctggagcg gctattgatt gagactgatt gtccctttct
     1621 tgtgcccgaa ccattgcgag gaagggcttc ggtatgtctt ccatcacata tagccctcac
     1681 agggaccttt cttgctaagt tgcggaatgt cccagtccct gatctcgcac aagtaacga
//
LOCUS       DAOPTR010000915         1739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_53659_length_1739_cov_2.676366, whole genome shotgun sequence.
ACCESSION   DAOPTR010000915 DAOPTR010000000
VERSION     DAOPTR010000915.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53659_length_1739_cov_2.676366"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="PKI05_14475"
     CDS             complement(<1..1181)
                     /locus_tag="PKI05_14475"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOA53455.1"
                     /translation="MAEPIISVSGLRGIIGESLTPEVAMRFAAAWAATAPAGAIVLAR
                     DGRASGTMIHAAVLAGLLGTGRTVLDANIAATPTVGVLVQHYRAAGGIQITASHNPPE
                     YNGLKLFSSEGRVIPSTLGQEVIKRYREGRLEWVRAARVGTREICPDTVTPHLNAILK
                     TVDVNRIRACHFRVLLDSNHGAGSVLGLPLLEALGCEVVPRGEIPNGAFEHPPEPLAE
                     NLLSICDAVRQCQADIGFCQDPDGDRLAIIDASGRYLGEEYTLSLCLDHRLKTRKGPV
                     VTNCASSRMNQDIAQKYGVPFFRSAVGEANVVDAMIAHDAVFGGEGNGGPIDPRVVLV
                     RDSFVGMACVLDAMASTGCTVAELAANLPRYAIVKTKVEFPTEKLEAAYHAVMRQFRD
                     AR"
     gene            complement(1425..>1739)
                     /locus_tag="PKI05_14480"
     CDS             complement(1425..>1739)
                     /locus_tag="PKI05_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53456.1"
                     /translation="LVRGNRANNLGVFLEAICLLRDSPRNNPAERRTNRLFPSRRTRP
                     EQAIVGPFSVFSVLENARSIAPNCYPRLLSIILVVCPPPWNNPNYPNYLFAYLLSGHS
                     VH"
BASE COUNT          400 a          489 c          479 g          371 t
ORIGIN      
        1 cgagcgtcgc gaaattgacg catcacagca tgataggcgg cctcaagttt ttctgtggga
       61 aactccactt ttgtcttgac aatcgcataa cgaggcaggt tggccgccaa ttcggcaacg
      121 gtgcaccccg tactcgccat agcatccagg acgcacgcca tacccacaaa gctatcccga
      181 actaggacga cccgcggatc gatgggacct ccgttaccct cgccaccgaa gaccgcatcg
      241 tgagcaatca tggcatccac aacgttggcc tcgcccacgg cgctgcggaa aaaaggcacg
      301 ccgtattttt gtgcgatgtc ctgattcatc cggctactag cgcaattggt gaccaccgga
      361 cccttgcgag tcttaagacg atgatcgaga cacaaagaga gggtgtattc ttcgccaagg
      421 tatcgtccgg acgcatcgat gatggctagc cgatctccat cgggatcctg gcagaacccg
      481 atgtcggctt ggcattgccg tacggcatcg cagatactca ggagattctc ggcgagcggt
      541 tccggcggat gctcaaaggc cccgttggga atttcaccgc ggggcaccac ttcacagcca
      601 agggcttcta acaagggcaa tccaagcaca ctgccggcac cgtgatttga atccaggagg
      661 acccggaaat ggcaagcccg gatgcggtta acatcgacgg tcttaagaat cgcattcaaa
      721 tggggagtaa ccgtatcggg acagatttca cgagtcccga cgcgggccgc tcgtacccat
      781 tccaaacgcc cttcgcgata gcgtttgatc acctcttgtc cgagtgtgct tggaatgaca
      841 cggccctcgg aactaaacaa ttttagtccg ttgtattcgg gcggattgtg gcttgccgtg
      901 atttgaatgc cacccgccgc ccgataatgc tgcaccaaaa caccaacggt aggggtggcc
      961 gcaatgttcg catccagcac agtacggcca gtgcccagca gccctgcgag caccgccgca
     1021 tggatcatgg tcccgctggc ccgtccatcg cgggcgagga cgattgcccc tgccggtgca
     1081 gttgcagccc aagccgcggc aaatcgcatg gccacttctg gcgtgagact ctcgccaata
     1141 ataccccgca agccagaaac gctgataatc ggttctgcca ttgtgcaacc tctttcccaa
     1201 ggaaccgtgg acctcagaag tccatcttac cccgtctttt gggggttgat cgcttgttac
     1261 ccgcggcttt tgtccctaca atgcaaaggt cggatgatag tacgtccggt ccgttgcagg
     1321 aattggcgtt agatggtgaa ggtattcgta attattgcga tttcgcgggc caggacgaag
     1381 tacaatcgca atcgtgatgg cggaatctta cacaaactgg cggttcagtg tacagagtga
     1441 ccgctgagca aataggcaaa caggtagttt gggtagtttg ggttattcca aggaggtgga
     1501 cacacaacca gaatgatgct gagcagtctt gggtaacaat tgggtgcaat tgatctcgcg
     1561 ttttcgagca cgctgaacac agagaaaggt ccaacgatgg cttgttcagg ccttgtcctc
     1621 cgcgagggaa aaagccggtt ggtccggcgt tccgcagggt tattcctcgg cgaatctcgc
     1681 agaaggcata tcgcttccag aaaaacaccc aggttgttcg ctcggtttcc tcgaacaag
//
LOCUS       DAOPTR010000916         1734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_53860_length_1734_cov_3.111971, whole genome shotgun sequence.
ACCESSION   DAOPTR010000916 DAOPTR010000000
VERSION     DAOPTR010000916.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53860_length_1734_cov_3.111971"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(340..1080)
                     /locus_tag="PKI05_14485"
     CDS             complement(340..1080)
                     /locus_tag="PKI05_14485"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOA53457.1"
                     /translation="MPGGIATYVFGGLSAICMGVSKTGVPGASILAVVLMAEAFRDNA
                     AFSVGALMPVLLVGDVFAVSLYAKDTAWSGLLRLFPYVALGLISGAFLLRYVQGNDLR
                     PVLGGLIVLMFGLEVYRRYRGENHTPHAWWFLLLTGWLAGFSTMVGNAAGPVMTMYLL
                     SQDLPKARFMGTCAVFFFTVNLLKTVPMAWVGMITLDTFRFALFAIPMTFIGLALGRF
                     LFCRMSQQVFDTVALLLAGIGGVWLLIP"
     gene            complement(1106..>1734)
                     /locus_tag="PKI05_14490"
     CDS             complement(1106..>1734)
                     /locus_tag="PKI05_14490"
                     /inference="COORDINATES: protein motif:HMM:NF013875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53458.1"
                     /translation="HEYALVYGLETLLRQGYNAGACLNPLIGETKGDDVPADVRMRTY
                     EALIEQRALGDGDSDPNLWGPRNEAVPDRVILVGLDIKMFYAGPKEAVMHAIYRQNFG
                     FTDIIIGRKHADAPYHDGTPIWGDFDAQEIFKRLRGDLKIKPLCVGFAAYYESVGRVD
                     LMENHPGEKPVSISGKEIRAALREGRPVDPRIMRASTARILAEAMANK"
BASE COUNT          443 a          542 c          427 g          322 t
ORIGIN      
        1 cttggtcgca tcgtggggag gcgtcgcctt gaccttctct gtcgtcacct gagagagtcc
       61 ctcatcaagc cgccgagcag cattatcctg ccgtgtgcat cccagcatcg ctacaacaca
      121 ggccaaaatc gccacaaaca catcaacgtt tttactgatt agcttcatga tttgctctga
      181 ttcacattaa gtctggcaaa aacgacacag cagtgatccc ccgatcccgt ccattgtagc
      241 tctgtccttc ctcggaacgc agcaacggag tgcttacacc tttggcaaac atggcaaagc
      301 aaaaccggcc aactgcaata gcgaaaatga cacgcccctt cagggaatga gaagccacac
      361 gccaccgatt cccgctagca atagggccac tgtatcaaac acctgctgcg acatgcgaca
      421 gaacaggaac cttcccaagg caaggccaat gaacgtcatg ggaatcgcaa acagggcaaa
      481 gcgaaatgta tccagggtga tcatccccac ccaggccatc gggacagtct tcaagagatt
      541 cacggtgaag aaaaacacgg cacaggtgcc catgaaccgg gccttgggca gatcctggct
      601 gaggaggtac atcgtcatca ccggcccggc agcgttgccc accatcgtgg aaaagcccgc
      661 caaccaaccc gtgagaagca aaaaccacca cgcatgtggc gtgtgatttt caccccgata
      721 ccggcgatag acctccagac caaacatgag gacgatcaat ccgcccagca ccggccgcag
      781 gtcgtttccc tgaacatagc gcaaaaggaa tgcgccagaa atcagtccca acgcgacata
      841 aggaaataac cgcaggagac cactccacgc ggtatctttg gcataaaggc tcacggcaaa
      901 gacatcgccc accaaaagca cgggcatcaa agcacccacc gagaaagcgg cgttgtcccg
      961 aaatgcctcc gccatgagaa cgacagccaa gatcgaggct ccgggaacgc ccgtcttgga
     1021 aacccccata cagatagccg aaagcccccc gaagacgtat gtagcaattc ctcccggcat
     1081 gaaatgggac tcccggtgag cgatgtcatt tgttggccat ggcctcggcc aaaatccgcg
     1141 ccgtactcgc ccgcatgatt cgtggatcga ccggccgacc ttcccgcaat gccgcccgga
     1201 tttctttacc cgaaatcgag acgggctttt cgccgggatg gttttccatt agatcaaccc
     1261 gccccactga ctcgtagtag gcggcaaaac ccacgcaaag gggtttgatc tttagatcgc
     1321 cgcgaagccg cttgaaaatc tcctgggcgt cgaagtctcc ccaaatcggc gtgccatcat
     1381 gatacggggc gtccgcatgc tttcgcccaa taatgatgtc cgtaaaccca aagttctgcc
     1441 ggtagatcgc gtgcatgacg gcctctttgg gcccggcgta aaacatcttg atgtccaagc
     1501 caacgagaat gacacgatcc gggaccgctt cgtttcgggg gccccacaag ttgggatcgc
     1561 tgtcgccgtc tcccaaggcc ctctgctcaa tcagggcctc gtacgttctc atgcggacat
     1621 ccgctggaac atcatcgccc tttgtctcgc caattaaggg gtttaagcac gctccggcgt
     1681 tgtaaccttg ccgaagcaac gtctctaggc cataaaccag agcatactcg tgag
//
LOCUS       DAOPTR010000917         1733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_53901_length_1733_cov_2.884982, whole genome shotgun sequence.
ACCESSION   DAOPTR010000917 DAOPTR010000000
VERSION     DAOPTR010000917.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53901_length_1733_cov_2.884982"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1218
                     /locus_tag="PKI05_14495"
     CDS             142..1218
                     /locus_tag="PKI05_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008655013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOA53459.1"
                     /translation="MTRCYALRESSPQATALTRHVAVSQNVIPRREVLGYMAAFGSAF
                     VAAQGLVSNAEAEELSRQQEVPPPRKRYEMKKSINLWALPYPAKMSLRECLQLCKEAG
                     FEGVELNYALDGDLTPETSDAAVKDVAKMVRTIGLEVSGLCSFLFWPYSFTHQDPERR
                     RRGVELAIDMIRVAQLLGTKNLLVIAGSTYVPWLPDDPPVPFDVCERRSREAMLQILP
                     VAEKAGISLNVENIFMNGFLHSPQELVSYVDSFKSPNVGIHFDTGNIGQYHFAEHWIP
                     PLGSRIRNVHLKEVTKKVNEFHLHTFRPLLDGTTNWPAVLEALDQVGYRGYLTFEYFN
                     PYPHWPEALVFHTSDALDRMLGRK"
     gene            1299..>1733
                     /locus_tag="PKI05_14500"
     CDS             1299..>1733
                     /locus_tag="PKI05_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53460.1"
                     /translation="MIPSATNTFSTVRKVFSLTCGLLVLSGWLAQAEEPGVIQVGTAA
                     VVITPPLGTPMAGYYSARGSEGVIDDLYVKAMVLDDGQTRVALVSCDLIGIPANVVAA
                     AREKITAATSLPGEHVLISSSHTHTGPVLRRDSRRDEMDGGTS"
BASE COUNT          342 a          479 c          526 g          386 t
ORIGIN      
        1 cagagcgcgt ccgttcaaac ggcaggacgc ggaggaccgc gtccctccca tgttgagatg
       61 gaggaccacg ctttgtcgtg gccggatgaa ctaacgcgga ggtttgttta ggcgattcac
      121 cggatacagg agttgcggac aatgaccagg tgttatgctt tgcgcgagtc gtctccccag
      181 gcgacggctt tgacacgtca tgtggcggtg tcccaaaatg tcattcccag gcgggaggtg
      241 cttggctata tggcggcctt tggaagtgcg tttgtggcgg cgcaggggct ggtttcaaac
      301 gcggaggcgg aggagctctc ccgtcagcag gaggtcccgc ccccacgcaa gcgctacgag
      361 atgaaaaagt ctatcaatct ttgggccttg ccctatccgg ccaagatgag cctccgggag
      421 tgcttgcaac tttgcaaaga agccggtttt gagggtgtgg agctcaacta cgccctcgac
      481 ggggatctta cgcccgagac ttccgatgcc gcggtgaagg atgtcgcaaa aatggtccgc
      541 accattgggc tggaagtcag tggcctgtgc tcgttcctat tttggccgta ttccttcacc
      601 catcaggacc cggaacggcg acgccgcggg gtggagctgg cgatcgacat gatccgtgtg
      661 gcccaactgc tcggaacgaa gaatcttctc gtcattgccg gctccacgta tgttccttgg
      721 ctgcccgacg atccgcccgt cccattcgac gtctgtgaac gccgatcacg cgaggcgatg
      781 ctgcaaatcc tgcctgtggc cgagaaggcg ggcatctccc ttaatgtgga aaacattttt
      841 atgaacggct tcctccattc gccgcaagag ctggtgagtt atgtcgatag ttttaagtcg
      901 ccgaatgtcg gcatccattt tgacacgggc aacattggac agtatcactt cgcggagcac
      961 tggattcccc cgctcggttc gcggatccgc aacgttcacc tgaaggaagt caccaagaag
     1021 gtcaacgaat tccacctgca cacatttcga cctctcctcg atggcaccac caactggccg
     1081 gccgttctcg aagcgttgga tcaggtgggc tatcgaggtt atttgacgtt tgagtatttc
     1141 aatccttatc ctcattggcc ggaggccctg gtgttccaca catcggacgc cctcgaccgg
     1201 atgttgggaa ggaaataagg aattggtttt gcgcgtcggc cttgacaggg agggcgatcc
     1261 tctcagcatg tcgtctccgc caaaaaggag ttgcaccaat gatcccgtcg gcaacgaata
     1321 cgttcagtac ggtaaggaag gtcttcagcc tgacctgcgg tctgctggtt ctttcgggtt
     1381 ggcttgccca ggcggaggag ccgggggtta tccaagtggg cacggccgct gtggtcatca
     1441 cgccaccgct ggggacacct atggcgggtt actattccgc ccgtgggagt gaaggggtga
     1501 tcgacgatct ttatgtcaag gccatggtcc tggacgatgg tcaaacccgc gttgcccttg
     1561 tctcttgcga cttgatcgga attccggcga atgtggtagc ggctgcccgc gaaaaaatca
     1621 ctgctgcgac gtcgctgccg ggagaacacg tcctgatctc ttcgagccac acgcacaccg
     1681 ggccggtttt gcgacgggat tcccgaagag atgagatgga cggaggcacg tcc
//
LOCUS       DAOPTR010000918         1733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_53905_length_1733_cov_2.651967, whole genome shotgun sequence.
ACCESSION   DAOPTR010000918 DAOPTR010000000
VERSION     DAOPTR010000918.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53905_length_1733_cov_2.651967"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PKI05_14505"
     CDS             <1..610
                     /locus_tag="PKI05_14505"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008681289.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOA53461.1"
                     /translation="EITETQVRAIFEATAELLKEGKSPHPEIMVPVTCDVKELDITKV
                     IVDRIHAEVEKKFGVTIKYLYGTMIEIPRACLVADKMAKTAQFFSFGTNDLTQMGFGF
                     SRDDIGGFLPDYLEKRILMADPFQTIDQEGVGQLIEMAVQKGRAARPDLKIGICGEQG
                     GDPESVEFCFRVGLNYVSCSPYRVPIARLAAAQASIKASRKK"
     gene            802..>1733
                     /locus_tag="PKI05_14510"
     CDS             802..>1733
                     /locus_tag="PKI05_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53462.1"
                     /translation="MSGSRRRHKSEEKDKLIATRPRSRERPQDTPVETEARIIRRVRL
                     FFLAFVTVILGARPLFPSEGADLGDGVAVIMSLLGGIVVGMTFLLGRSRLTIRLGWAD
                     AAVLFVVLLAIIASIRGALVGTARPAINMAWEWLGYGLLWLLVRQLRLDAKERRIILA
                     GMASIAVGVAAYGLYQYLIEFPAMREAYQANRIEWLRSLGWDDLQTNPTWLEHFENRL
                     YSREPLATFALANSLAGFVLPWCVLLLAAFLESGMIRGSSTQLTNPQSPSERPRPFGK
                     GQETRFRGVQLSRLVRFLWPVGTLIPLSLAGVCLV"
BASE COUNT          352 a          460 c          515 g          406 t
ORIGIN      
        1 cgagatcacg gagacgcaag tgcgggccat ctttgaggcc acggcggaat tgctcaagga
       61 agggaagtcg ccccatccgg agatcatggt gcccgtcacg tgcgatgtga aggaactcga
      121 catcaccaag gtcattgtgg accggattca tgccgaggtt gagaagaaat ttggggtgac
      181 gatcaagtat ctctatggaa caatgatcga aatcccgcgg gcctgcttgg tggccgacaa
      241 gatggccaag acagctcagt tcttctcctt cggcaccaat gaccttactc agatgggctt
      301 cggcttcagt cgggatgaca ttggcggctt cctgccggat tatctcgaga agcgaatcct
      361 catggctgat cccttccaaa ccatcgatca ggaaggcgtt ggccagttga ttgaaatggc
      421 tgtgcaaaag ggtcgggcgg ctcgaccgga cctgaagatt ggaatctgcg gtgagcaggg
      481 cggtgatccc gagtcggtcg agttctgctt ccgtgtcggc ctgaactacg tgagctgctc
      541 gccctatcgt gtgccgattg cccggttggc agccgctcag gcctccatca aggccagtcg
      601 caagaaatga tgttctagac atcgccgtct aggctggatt gtaaaacagg ctacaggagg
      661 tgttaccaac gacttcctgt agcctttttt cttcaaatcg tttgccgaac atgcccaacg
      721 atgtcgtccc cagctttgtg gtgtaagcta aagcatagag gaaacaacca tcaccacctg
      781 cgggatactt gaatttaggc gatgtcgggt tccagacgcc gtcacaagag cgaagaaaaa
      841 gataagctta tagccacacg gccacgttcg cgcgagcggc cacaggatac gccggtggaa
      901 accgaggcac gcattatccg gcgggtccga ttgttcttcc tggcgtttgt tacggtgatc
      961 ctgggggcca ggcccctttt ccctagcgag ggtgccgacc tcggtgacgg tgtggctgtg
     1021 atcatgtccc ttttaggtgg gattgtcgtc ggtatgacgt ttctgctggg caggtcccgc
     1081 ctgacgattc gcctgggatg ggccgacgcc gcggttctct tcgtggttct cctggcgatt
     1141 atcgcctcaa taagaggtgc gctggtaggc accgcccgac cagccatcaa tatggcgtgg
     1201 gaatggcttg ggtacggctt gctttggctc ttagtacggc aactgcgact ggacgcaaag
     1261 gagagacgaa ttatcttggc cgggatggcc tccatagctg tgggcgtggc tgcctatggc
     1321 ctgtatcaat atctaatcga atttcccgca atgcgagagg cctatcaggc gaatcgaata
     1381 gagtggctgc gttccctggg gtgggacgac ctgcaaacga atccgacttg gttagagcac
     1441 tttgaaaacc gactttacag ccgtgagcca ctcgccacat ttgccctggc caattccttg
     1501 gcgggattcg tccttccttg gtgcgtcctc cttctggcgg cctttctcga gtcgggaatg
     1561 atcaggggca gctcgactca actgacgaac cctcaaagtc cttctgaaag gccacgcccg
     1621 tttggcaaag gccaggagac ccgtttcagg ggtgtccaat tgtcgcgact tgttcgtttc
     1681 ctctggcctg ttggcacgct gatacctctc agcctagcgg gggtgtgttt ggt
//
LOCUS       DAOPTR010000919         1729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_54054_length_1729_cov_4.141577, whole genome shotgun sequence.
ACCESSION   DAOPTR010000919 DAOPTR010000000
VERSION     DAOPTR010000919.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54054_length_1729_cov_4.141577"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="PKI05_14515"
     CDS             complement(<1..1274)
                     /locus_tag="PKI05_14515"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HOA53463.1"
                     /translation="MSLDQTPGAEDDRPVRRVANVRDFESFCKEVRRRPRLALDTEFI
                     AEDSYQPNLCLIQLAFDDELVVIDPFPLGSVRRFWEAIVEGNVETVVHAGRTDLQFCR
                     EATGRCPERIFDVQIAAGFTSQEYPAGLVNILQKYAHVSVTKHETRTDWRRRPLSDRQ
                     IEYALNDVRYLLTVRDQLEAELKDLGRYEWFLEEVRYQYTECVSEDSEPWRRLAGCAG
                     LNRRELAILKELWFWREEEAARRNVPPRRVLRDDLLIELSRRGSGDPKRIYAVRGMTN
                     RGVQQLVPEIARRIQQALNTPREEWPEVINRTGIGQSPIVGQFLYAALASLCRKERIA
                     VGLVGGTNDLRDWFSYYVSGREGPPPLLARGWRASLIGDTLERLLSGKVGLRIHNCFD
                     DNPLELVPLSPETSSQGGNNVLPETLQQLPQDV"
BASE COUNT          384 a          507 c          446 g          392 t
ORIGIN      
        1 acatcttgag gaagttgttg cagtgtctcc ggtaacacat tgttaccacc ttgagacgac
       61 gtttccgggg acagagggac cagttcaagg ggattatcgt caaaacaatt atgaatccgc
      121 aggcccactt tgccgctaag aaggcgctcc aacgtgtcac caatgagaga tgcccgccac
      181 cctcgagcaa gtagcggcgg cggtccttct cgtccggaaa cataataact gaaccaatcc
      241 ctgaggtcgt tggttccccc gacgaggccc actgcgatcc gttctttacg gcagaggctg
      301 gccaaggcag catataagaa ctgcccaaca atcggtgatt ggccaatacc tgtacggtta
      361 atgacttccg gccactcttc ccgaggtgtg ttgagggcct gttggattcg acgggcaatc
      421 tctgggacaa gctgttgcac gcctcgattc gtcatcccac gaacagcgta aatccgcttg
      481 gggtccccgg acccccgccg ggaaagctcg atcaagaggt catcgcgcag cacacgtcgg
      541 ggtggcacgt ttctacgggc ggcctcctct tcccgccaga accaaagctc cttaaggatg
      601 gcaagttcac gtcggttaag tccagcacaa ccggccaatc gccgccaagg ttcagagtcc
      661 tcgctcacac attccgtata ttgataacga acttcttcga gaaaccactc atatcgcccc
      721 aggtccttga gctctgcttc gagctggtct cgtaccgtca gcagataacg gacgtcgttc
      781 aaggcgtact cgatctgtcg atcactcagc ggacggcgcc gccagtcggt tcgtgtttcg
      841 tgttttgtta cgctaacgtg agcgtacttt tggaggatat tgaccaggcc tgcaggatac
      901 tcttgacttg taaaccccgc ggcaatttgg acatcgaaaa tacgctcggg acatcgtccc
      961 gttgcctctc ggcagaattg gaggtccgtt cgacccgcat gcacaaccgt ttccacgttc
     1021 ccttccacaa tggcctccca aaatcgccga acagaaccaa gggggaaggg gtctatcacc
     1081 acaagttcat catcgaaggc aagttgaatt aggcagagat tcggttggta gctgtcttcc
     1141 gcaatgaatt cggtatcgag tgccaaacgc ggccgacggc ggacttcttt gcagaagctt
     1201 tcgaaatcac ggacattagc gacccgccgt accggacgat cgtcttccgc accgggcgtt
     1261 tgatccaaac tcacgtttga ccccccatga ctgcagcgag cattaagaca cccttttaat
     1321 gaggtcgggc ccccacatag gagaaatacc cacagggagg gccggtgtca aatcactttt
     1381 accccaagaa ggccaatcgg cgttcttccg ctccttttca gaattcccca aattctcaag
     1441 atagcattaa ctcaaaaaca gggagcggca cccaatgttt gcggagacca cgcccctctc
     1501 tgtcctctca attagagcct atcccataat ctcccctcgc ccgttcggcg ggagaagggg
     1561 cgggggtgag ggtggctcga atcgctgccg gttttctcaa tcgattctca cgttcgtttg
     1621 ccctcgccct acccctctcc cagagggaga gggggttttt gggatggact cttggctttc
     1681 gcgtgatgtt gggttctcga cgttttccgt acgatagccg attaccgga
//
LOCUS       DAOPTR010000920         1727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_54148_length_1727_cov_2.863038, whole genome shotgun sequence.
ACCESSION   DAOPTR010000920 DAOPTR010000000
VERSION     DAOPTR010000920.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54148_length_1727_cov_2.863038"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PKI05_14520"
     CDS             complement(<1..275)
                     /locus_tag="PKI05_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53464.1"
                     /translation="MSEELASSDAPELPVQAPSAQQVAEELSRLRRSIRRLTVLVSLL
                     TLAVFLCSAAVFGELVNYFSFDPMLYGGASAGAAILGFIFGLIVGRR"
     gene            complement(348..1154)
                     /locus_tag="PKI05_14525"
     CDS             complement(348..1154)
                     /locus_tag="PKI05_14525"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOA53465.1"
                     /translation="MSHIRWLLVRDAVRRVSRHAAYAWLVGIGVVACSLMTAAVLAEQ
                     GGPSAAGDQEQVVDLSAKDLTGWREPHGQWAIVGEAVMDPQEPKRLAVKPGTGVLYNG
                     PQGRTVNLLSVAEHGDVIAHIEFMVPKGSNSGVYFQGRYEIQVFDSWGVEKPSYSDCG
                     GIYQRWGPQGGYEGHPPRVNASRAPGEWQTFDVIFRAPRFDENGKKIENARFVKVVHN
                     GVVVHENVEVTGPTRSAHFDDEKPTGPLMLQGDHGPVAYRNIRLRLVKLP"
     gene            1451..>1727
                     /locus_tag="PKI05_14530"
     CDS             1451..>1727
                     /locus_tag="PKI05_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53466.1"
                     /translation="MSEGSAISSQERWYRLLTGYQWFVLIVCTAGWAFDTFDQQLFNL
                     ARQPAVRDLLGSATTDVVNFYGGLATAVMLIGWATGGIIFGIMGDKIG"
BASE COUNT          381 a          492 c          471 g          383 t
ORIGIN      
        1 cagcggcgac ctacaataag cccaaagata aatcccaaga tcgcagcgcc ggccgatgcg
       61 cctccgtaaa gcatgggatc gaaagaaaaa tagttgacca gctcaccaaa cacggcggcg
      121 gagcaaagaa acaccgccag cgtgagcagc gagaccaaga cggttaacct gcgaatgctc
      181 cgccgcagtc ggctgagttc ctcggcaacc tgttgtgccg aaggggcctg cacaggcagt
      241 tccggggcat cactcgaagc cagctcttcg gacatcgaag cgacctctcc tcggttgttg
      301 ttttgtatga cttgcgcgcc acaccttgtg gaccggcgtc tgtcttccta gggaagtttt
      361 accaggcgta gccgaatgtt tcggtaggca acgggcccat gatctccctg gagcatgagc
      421 ggtccggtcg gcttttcatc gtcaaaatgt gccgaccggg tgggaccagt gacctccacg
      481 ttctcgtgaa caaccacgcc attgtggacg actttcacaa accgcgcgtt ttcgatcttt
      541 ttcccattct cgtcaaagcg tggggcgcgg aagatcacat cgaacgtttg ccactcgccc
      601 ggtgcccgcg atgcatttac ccgtggggga tggccctcat atccgccttg cggtccccag
      661 cgctggtaaa tcccgccgca atcgctgtaa ctgggctttt ccacacccca gctatcgaag
      721 acctggattt cgtagcgacc ttgaaaatag acacccgagt tggaaccttt cgggaccatg
      781 aactcgatat gggcgatgac gtcgccatgt tctgcgacac taagcaaatt gaccgtcctc
      841 ccttgtgggc cgttgtacaa gacacccgtt cccggcttga cagcgagccg tttcggttct
      901 tgtgggtcca tcactgcttc accaacgatg gcccattgcc cgtgcggttc acgccagccg
      961 gtcaagtctt ttgccgatag atcaacgacc tgctcttgat cacccgccgc gcttgggccg
     1021 ccttgttccg ccaggacagc ggctgtcatc aaactgcaag cgacaacgcc aatgcctact
     1081 aaccaagcgt aagccgcgtg ccgacttacg cgacgaacag catcccgaac cagaagccat
     1141 cgaatgtgcg acatggcatg ttcctttaca tgtgaccaca gccttcggaa agcattgcat
     1201 caattagaaa ttaaaaggca cgcatttctc cgcgtcaaca cgatgccttc gcaattaacg
     1261 gatgaagagt ttttggggga cctttgggac tggacaggcc aactgttttg gttcataatc
     1321 ccactaaaat tttcctctgg gccgagaaac tcggcggaag gcttttgccc aaaggagaag
     1381 tgtggggttt gtgcccggca cggcatacgg tcaccgcttt gccgaacaag gcgaataaaa
     1441 ggagaagagc atgagtgaag gttcggccat ttccagccag gagcgttggt atcgtctcct
     1501 gaccggttat caatggtttg tcctgattgt gtgcacggcc ggttgggcgt ttgacacgtt
     1561 tgaccaacaa ctgttcaatc ttgcccgaca accagccgtc cgcgatctgt taggatcggc
     1621 cacgacagac gttgtcaatt tttacggcgg tttggcgacg gccgtgatgc ttatcggctg
     1681 ggcgacgggg ggaattatct ttgggatcat gggggacaag attgggc
//
LOCUS       DAOPTR010000921         1723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_54305_length_1723_cov_4.380096, whole genome shotgun sequence.
ACCESSION   DAOPTR010000921 DAOPTR010000000
VERSION     DAOPTR010000921.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54305_length_1723_cov_4.380096"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(492..>1723)
                     /locus_tag="PKI05_14535"
     CDS             complement(492..>1723)
                     /locus_tag="PKI05_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOA53467.1"
                     /translation="GYYITRIAPQKEKKEKAQKAKSRKTFSIGRVNAKTLTTFTKQLS
                     VLQDAGLPILRSLKILEAQAKPSLFGSLKNCLIDVIEEIEGGATLSEAMAKAPKAFDR
                     LYVNMVKAGEAGGALEVILRRLAEFQERALWLRRKVKGALVYPIAVIVFACLILTFIM
                     IRIVPQFQKIFEEFETDLPVPTQILITASQTVIHWWFLLPGVPLGIWLFVKLTTMFPY
                     AKFGLDLFKLKLPIFGQLVEKNILARTTRTLGTLVASGVPILEALTITRDTSTNAVFE
                     RLYQRVLDAIREGETIAKPLKDNSRPGFHPVTAWWFFFFLGGPIGLLLYLTRWNNPII
                     EDIAINMVDVGEETGELDTMLYKIADNYDEEVAIITESLLSLFEPILIIVLGVMVGFI
                     VVALFLPLIALIQKLSG"
BASE COUNT          408 a          495 c          440 g          380 t
ORIGIN      
        1 ggcttccctg gccctggccc gcgcgtaaat cgtggcggct gagatgagcg ccgccaaaat
       61 cccgatgatg acgatgaccg tcagcatttc cacgagcgtg aagcctggcc ggccacctgc
      121 aacccctatc cggtagccgt tttgattcaa ctgattgcgt ttcatagggc atcccccgta
      181 tcgacgttca atcttctctg gcctagtgaa ttctaaggat gttctcgtat cgaaagtgat
      241 ttcgccacga acttcgttgg tttcttgtgg tgaggcgcac gaggcgacaa ggcaccttat
      301 gtcggccaaa gcacacctac gcctccggcg ttccgcttaa tagacgtctc cgatgcccaa
      361 agttaatcac cgggacgaaa acgcttactg cgtttcgccc attggtttga tccgcccctt
      421 gcccgggggc gttttcgctg gcccgtgtta agattagcct cggtaatcca ccaccagacc
      481 tccccgccat attacccgga caatttctga atgagggcaa tcagcgggag gaatagggcg
      541 accacaatga agccaaccat cacccccaac acaataatca ggatgggctc aaacaagctc
      601 aaaaggcttt ctgtgatgat ggccacctct tcgtcgtagt tgtcggcgat cttgtacaac
      661 atggtgtcca actcaccggt ttcctcgccg acatccacca tgttaatggc aatgtcctcg
      721 atgatgggat tattccaacg cgtcaagtag aggagcagcc caataggtcc tcccaggaag
      781 aagaagaacc accacgccgt caccggatga aagcctggtc gagaattatc cttaagcggc
      841 ttggcgatag tttcaccttc ccgaatggcg tcgagcaccc gctgatacag ccgctcaaac
      901 acggcattgg tggacgtatc ccgcgtaatc gtgagggcct ccaagatggg cacaccactg
      961 gccaccaggg ttcccaaggt tcgcgtggta cgtgccaaga tgttcttctc cacaagttgt
     1021 ccaaagatcg gcaacttgag tttaaacagg tccaagccaa atttggcgta gggaaacatc
     1081 gtcgtcaact tgacaaagag ccagattccc agcggaacac ccggcaggag gaaccaccaa
     1141 tggatgaccg tttgagaagc cgtgataaga atctgcgtgg ggaccggcag atcggtctcg
     1201 aactcttcga aaatcttttg gaattgcggc acgatcctga tcatgatgaa ggtcaagatc
     1261 aagcacgcaa acacaatgac ggcgatagga tagaccaagg cgcctttgac cttccgccgg
     1321 agccagaggg cccgttcttg gaactctgcc agacgccgca gaatgacttc caaagcacca
     1381 ccggcctcgc cagctttcac catgttcaca taaagccggt caaaggcctt gggggcctta
     1441 gccatcgctt cagagagcgt agccccgccc tcaatctcct cgatcacgtc gatcaagcag
     1501 ttttttaggc tcccaaacaa cgagggctta gcctgggcct cgagaatctt caaactccgc
     1561 aggatgggca agccggcatc ctggagcacc gaaagctgct tggtgaaggt cgtaagggtt
     1621 ttggcattga ccctaccaat ggaaaaggtt ttacggctct tggccttttg ggccttttcc
     1681 tttttttcct tctgaggggc gatccgcgtg atgtaatacc cca
//
LOCUS       DAOPTR010000922         1721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_54382_length_1721_cov_4.091837, whole genome shotgun sequence.
ACCESSION   DAOPTR010000922 DAOPTR010000000
VERSION     DAOPTR010000922.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54382_length_1721_cov_4.091837"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..1325)
                     /gene="istA"
                     /locus_tag="PKI05_14540"
     CDS             complement(129..1325)
                     /gene="istA"
                     /locus_tag="PKI05_14540"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOA53468.1"
                     /translation="MGISRNTVRRYLRDPDGALKRKPRAKRPSKLEPYAEYIMRRVAE
                     GVDNCVVLLREIREQGYTGGYTILKDFVRPLRHRAASRTTTVRYETEPGEQAQVDFGS
                     FSYRTPDGKLRQVWGFVMVLSWSRAIYVEFVPRATTEQFIRCHLNAFAKFGGIPKKCL
                     YDNTKLVVLSRDSGGAPVWNRQFLDFALRLGIEVQLCRPYRAQTKGRVESGIKYVKHN
                     FWPTARFSDLDDLNRQAWAWIDTVADVRVHGTTFERPIDRLEVERTSLTPLPDTERLR
                     PFLRERATVGRDGYVRWNRAWYGLPWPWKSGQKIEVEANGDLVFLWSGDELLAVHPKA
                     TKAGQRLTHPRQWSGMPQTTERRPIEPTAVQLPAVEVERRSLALYDLVTEVTSNDRSR
                     TGPSSS"
BASE COUNT          356 a          506 c          459 g          400 t
ORIGIN      
        1 gttctcgtcg ggccgccact tccacgctca acaagtcgag caggaagtcg acgtaggaaa
       61 gttctttatg tgccgcttgt tgtaatctgc tgtccagcac ttccgcagct tgcgtcaagc
      121 cgagctgttc aagatgaact cgggcctgtt cgagaacgat cattggacgt cacctccgtc
      181 accaagtcat agagcgccaa ggaacgccgt tcaacttcca cggcgggcaa ctgaacggcc
      241 gtaggctcga ttggccgtct ttcggttgtt tgtggcattc ccgaccattg ccggggatgg
      301 gttaagcgtt gaccggcttt ggtggctttt ggatggacgg caagaagctc atcacccgac
      361 caaaggaata ccaggtcgcc gtttgcctca acctcaatct tctgcccgct tttccacggc
      421 catggcagac cgtaccaagc ccggttccat cgcacgtatc cgtcgcgtcc caccgttgcc
      481 ctttcccgca ggaaggggcg gaggcgttcg gtatccggca agggggtcaa actggttcgc
      541 tccacctcga gtcggtcaat aggccgttca aaggtggtcc catggacgcg cacgtcggca
      601 accgtgtcga tccatgccca tgcttggcga ttaaggtcat cgaggtcgct gaagcgggca
      661 gttggccaga agttgtgctt cacgtacttg atgccgcttt caacacgccc cttggtctga
      721 gcccgataag gacgacaaag ctgaacctcg attcccagtc gaagagcaaa atcgaggaat
      781 tggcggttcc acaccggtgc acccccgctg tcccggctca acaccacaag tttggtattg
      841 tcgtaaaggc actttttggg gatcccgcca aatttggcga aggcattgag atggcagcgt
      901 atgaattgct cagtcgtagc acgcggcacg aactccacat atatggctct cgaccaactg
      961 agaaccatca caaatcccca cacctgccga agtttgccgt ccggcgtgcg gtacgagaac
     1021 gacccaaagt ctacttgcgc ttgttctccg ggttccgtct catagcgcac cgtcgtggtt
     1081 cggcttgcgg ctctgtgtcg caacggccgt acaaaatcct tgaggatcgt gtatccgccg
     1141 gtgtaacctt gctcccggat ttctctcaac agtacgacgc agttgtccac tccctccgca
     1201 acgcgtcgca tgatatactc ggcgtagggc tccaacttcg acggtcgttt ggcacgcggc
     1261 ttgcgtttca gggctccgtc gggatcccgc aggtatcgcc gtaccgtgtt tcgagagatc
     1321 cccagcgtct tggcgattta ccgaatcgat ttcttgcctt ccaactcata gatcgccttc
     1381 actcttccac cctccagcat tgttcttttc cacctccgac tcttgacggg aatcggagta
     1441 ttcttcgctg gggggtgggt catttcattc ccgcccatcc tgggtcagtt tatcaccgcc
     1501 gatgacacgc gcaaacgcag ggtatcggtg gttgggttcc tgcttgtagg tgatggttcg
     1561 acgccttctg cacccttgtc tatgcctact cggcatataa ccccgcatca aaagaggctc
     1621 aaatgaagga aaacggccag ttttggggca acggcccagg gatctaataa tagagaaaac
     1681 ggcaaaaaga aagaaggaaa tccttgaccg aggtatagcc a
//
LOCUS       DAOPTR010000923         1721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_54385_length_1721_cov_3.621849, whole genome shotgun sequence.
ACCESSION   DAOPTR010000923 DAOPTR010000000
VERSION     DAOPTR010000923.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54385_length_1721_cov_3.621849"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..>1721
                     /locus_tag="PKI05_14545"
     CDS             253..>1721
                     /locus_tag="PKI05_14545"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HOA53469.1"
                     /translation="MCHQTPLVEVVPRRGKSQIYAKVEPDDVPAIVHRHFRPRKLWSR
                     LRYGLNRWLDHLLGDIDEDALEPQSLDVRDPPVCAFLGRQLRIATELAGEIDPLSLDE
                     YLAHGGFKAAERAVKEWSAEQIIEEVKRSGLRGRGGAGFPTAEKWTVVRKATGEPKYV
                     TCNGDEGDPGAFMDRMILESFPYRVIEGMLIAAKAVGAHEGIFYIRAEYPLAVERVRA
                     AIHICEERGILGDRVLGTDFRFHLRVNEGAGAFVCGEETALIASMEGRRGMPRLRPPY
                     PAEFGFGGRPTLVNNVETFALVPWIIRHGAERFARLGTATSKGTKVFALAGKIRRGGL
                     IEVPMGITLREIIEEIGGGALPGRRIKAVQVGGPSGGCVSEALFDTPVDYEALTATGA
                     IMGSGGLIVLDNTDCMVDIARYFLRFTQDQSCGKCTLCRIGTRRMLEILERICNGEGR
                     AGDLEVLEDLARTVSAGSLCGLGKTAPNPVLTTLRYFRE"
BASE COUNT          328 a          430 c          581 g          382 t
ORIGIN      
        1 gatcggtcca cctatgggcg attgaccccg gcgaagatac cgcgggtatt ggcggacttt
       61 ctccacgccc agagcaacgg atcacaccct tctcgtgggc catcggctgg agactttgag
      121 gagggcgaag tacgggtcgg ccttggctcc tgttgccagg cccaaggaag cgcggaggtt
      181 tatcgacggt ttgaagaatt ggtgactctc gccgcggtac cgatttctat caagcgggtc
      241 gggtgcgtgg gaatgtgcca ccaaacgccg ctggtggaag ttgtgccacg gcgtgggaag
      301 tcccaaatct atgccaaagt tgagccagac gatgtgccgg ccatcgttca tcgacacttc
      361 cgcccgcgta aactttggtc caggctccgc tatgggctga accgctggtt ggatcatttg
      421 ctgggtgata ttgatgaaga cgcacttgag ccgcagtcgt tggatgtccg cgatcctcct
      481 gtgtgtgcct ttctggggcg gcaattgcga atcgctacgg agctagccgg tgagattgat
      541 ccgcttagtt tggatgagta cctcgcgcat ggtgggttca aggctgccga gcgagctgtg
      601 aaggagtggt cggcagagca gatcatcgag gaagttaagc ggagtgggct ccgcggccga
      661 ggaggggcag gcttccccac tgccgaaaaa tggaccgtgg tgagaaaggc tacaggcgag
      721 ccaaaatacg tcacgtgcaa cggggatgag ggtgaccccg gtgcctttat ggatcgcatg
      781 atcctggagt cgtttcccta ccgtgtcatc gagggtatgc ttattgccgc caaggcggtt
      841 ggggcacacg agggaatctt ctacatccgc gccgagtatc cgttagcggt agagcgggtg
      901 cgggcggcaa ttcacatctg tgaagagcgg ggtattttag gcgatcgggt gttgggtacg
      961 gattttcgct tccacctccg ggtcaatgaa ggggctgggg cgttcgtgtg tggtgaggaa
     1021 acggcgttga tcgcctcgat ggaaggacga cgagggatgc cccgactcag gcccccgtat
     1081 cctgccgagt tcggttttgg cggcagaccc accttggtca ataatgttga gaccttcgcc
     1141 ctcgtcccct ggattattcg gcatggtgcc gaacgatttg cccgtttagg gacggccaca
     1201 agcaagggga cgaaggtgtt cgccttggcg ggcaagattc ggcggggtgg cctgatcgaa
     1261 gttccgatgg gtatcacgct gcgggaaatt atcgaggaaa tcggtggcgg agcgctcccc
     1321 gggcgaagga ttaaagccgt ccaggtcggt ggtccctcgg gcggctgtgt gtctgaggcc
     1381 cttttcgata ctccggtgga ttatgaggcc ctcacggcga ccggggccat catggggtca
     1441 ggtggactca ttgtgctgga taataccgac tgcatggtgg atatcgcccg gtattttctc
     1501 cggtttaccc aagatcagtc gtgtggaaag tgtacacttt gccgcattgg aacacgtcgg
     1561 atgctcgaga ttctggagcg gatttgtaac ggcgaaggac gagcgggcga tctggaggtt
     1621 ttggaagact tagcgcgaac cgtcagtgcc ggaagccttt gcggtttggg caagacggct
     1681 cctaaccccg tcctgacgac attgcgctat ttccgcgagg a
//
LOCUS       DAOPTR010000924         1718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_54495_length_1718_cov_4.319302, whole genome shotgun sequence.
ACCESSION   DAOPTR010000924 DAOPTR010000000
VERSION     DAOPTR010000924.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54495_length_1718_cov_4.319302"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PKI05_14550"
     CDS             <1..282
                     /locus_tag="PKI05_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA53470.1"
                     /translation="LSSPALLAGRKVAEIGGYPATFHVRDFLDCVKTRGKPKANADVA
                     CHSHITCHAVNIAIFLNRKLRFDPVKYEFIGDDEANRLRGEALREPWRV"
     gene            365..>1718
                     /locus_tag="PKI05_14555"
     CDS             365..>1718
                     /locus_tag="PKI05_14555"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOA53471.1"
                     /translation="MRSLKCMIIVLGTVLLLTAPCWAQQQPAKEADLIALLQSDGPKA
                     EKALACKKLAVWGTEASVPALAAYLNDPELASWARIALEAIPGDAPNQALRASLGQLK
                     GRLLVGAINSLGVRRDEGAVAMLAEKLTDADADVAAAAATALGHIGGPQAVAVLTPAL
                     NTSTGIVKNAVAEGLLFCADRFLAEGKKQEAREIYDAVRKADVPKPRRVEATRGAILA
                     SGKEGLDLLAECLASNDKDFFAIGLSTARELKGAEVSKVLVGQLGPIQKGEEKKPALL
                     TIKKAEYGAGDRWADVTQAVAAAVRNNTLRITASNALAGDPAPGVVKQLRMVYAIGGQ
                     EQTIAVKENDSVVLGESVAPADPRQVAILEALGDIGDPVGIEAIVKAARDGSWGLRLA
                     AIRILGRVGDAQAVPVLVAAAAEGGELAQAAAEGLIQLPGSDVDAAIVEALRTAQGPV
                     R"
BASE COUNT          352 a          458 c          559 g          349 t
ORIGIN      
        1 ttaagctctc cagcactgtt ggcaggtcgc aaggtggccg agatcggcgg ctatcctgca
       61 acgttccacg ttcgtgattt cctcgactgc gtgaaaacac ggggcaagcc aaaggcaaac
      121 gctgatgttg cttgtcattc ccatatcacg tgccacgcgg taaacattgc cattttcctc
      181 aaccgaaaac ttcgctttga tcccgtgaag tacgaattta tcggggatga cgaagcgaat
      241 cgcctgcgcg gcgaggcact ccgcgagccg tggagagttt gatgggtgtt gaacgttggg
      301 tcgtaatttc ctcacttagc caaatcgata acatctgaga gttttcccag tcggaggata
      361 aaccatgcga agtttgaaat gtatgatcat cgtgctgggc actgtgttgc ttttaactgc
      421 tccatgctgg gcccaacagc aaccagcgaa agaagccgac ttgattgccc tcctgcagtc
      481 ggatgggcca aaggccgaga aggccctggc ctgcaagaag ctggcggtct gggggactga
      541 ggcatctgtt ccggccttgg ctgcctattt aaatgatcct gagttggcct cgtgggcgag
      601 aatcgccctg gaggccatcc cgggagacgc tcccaatcaa gccttgcggg caagcctcgg
      661 gcaattgaaa ggtcggctcc tggttggagc catcaactcc cttggggtgc gacgtgatga
      721 gggagccgtg gccatgttgg ccgagaagct caccgatgcg gatgccgacg tcgcggcggc
      781 tgcggcgaca gcgttagggc atattggagg tcctcaagcc gtcgcggtac tgacccccgc
      841 cttgaatact tcaacaggaa tcgtcaagaa tgcggtcgcg gaaggcctgt tgttctgtgc
      901 cgaccgattc ctcgcggagg gcaaaaagca ggaagcacga gaaatctacg atgctgtccg
      961 caaggccgat gttcccaagc cgcgtcgtgt ggaggccacc cgaggggcta tcctcgccag
     1021 cgggaaggaa ggtcttgacc ttttggccga gtgccttgcc tccaatgaca aggacttctt
     1081 cgctataggt ctttccacgg caagagagtt aaaaggggca gaggtgtcca aggtcttggt
     1141 tggacaactt ggtcccatac aaaagggcga ggagaagaag ccagcgctgt tgacgatcaa
     1201 gaaagccgaa tatggagccg gggatcgctg ggccgacgtg acacaggctg tggcggctgc
     1261 ggtgcgcaac aatacgcttc gtatcacggc ttccaacgcc ttggcgggag accccgctcc
     1321 cggtgtggtg aaacagttgc ggatggtgta cgccattggg gggcaggagc agaccattgc
     1381 ggtcaaggag aacgactcgg tggttctcgg ggagtccgtg gcaccagctg acccacggca
     1441 agttgccatt ctggaggccc tgggcgatat cggcgatccc gtggggatcg aagcgatcgt
     1501 caaggccgcc cgagacggaa gctggggctt acgactggcg gcgatccgca tcttgggacg
     1561 ggttggcgac gcccaggcag tccccgtttt agttgcagcc gctgccgagg gtggtgagtt
     1621 ggcgcaggcc gctgccgaag gtctcatcca gttgccggga tcggatgtcg atgcggcgat
     1681 tgtggaggcc ttgcgcacgg cccagggtcc ggttcgcg
//
LOCUS       DAOPTR010000925         1718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_54496_length_1718_cov_3.928443, whole genome shotgun sequence.
ACCESSION   DAOPTR010000925 DAOPTR010000000
VERSION     DAOPTR010000925.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54496_length_1718_cov_3.928443"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..>1718)
                     /locus_tag="PKI05_14560"
     CDS             complement(33..>1718)
                     /locus_tag="PKI05_14560"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOA53472.1"
                     /translation="AAGLRRLLTITARVRRKGKEIEVPAEEVVPGDIILLESGNKVPA
                     DMRLVEVNNLAADESFLTGESVASEKTTHILPEDTVVGDRKNMAFAGSTITSGRGVGI
                     VVATGSETQVGLIAKTVSESEMGKPPLVLRMERFVKQITILVLVISTFLAVLLWFQGN
                     DIPSIFFFVVALAVSAIPEGLPVALTVALSVATRRMSARNVIVRKLTAVESLGSCTVI
                     ASDKTGTLTVNQQTVRVVDLADGRTFEFTGEGYSGEGQVFRKGGEITPTDHEQLTRLA
                     EAAILANEGSLVREEGGWKHRGDAMDVALLAMGYKLGLSPTEARRQVKILFEIPYESE
                     HKYSAVFYEKNGTTYVAAKGAVETILDFCSGVLRDGTPLPLDRDDIERRAQAMAEKGL
                     RVLAVAGGQTESLPSEASDQAKFLSGLLFYGLVGFIDPLRPEAIEAVRTCKSAGIKVL
                     MITGDHPATAGAIARELGLSDIDEPVVTGAQLNEAGPTESPAFEKLVTSSHVFARISP
                     TQKLEIVDVLMREGELVAVTGDGVNDAPALRRANIGVAMGSGTDVAKLDFCQS"
BASE COUNT          370 a          549 c          432 g          367 t
ORIGIN      
        1 attcgaccaa ctctgccaag ccacgtaggc actcaagact ggcaaaagtc gagcttagca
       61 acatcagttc ccgatcccat cgccacacca atgttcgccc ttcgaagcgc cggtgcatcg
      121 ttgaccccgt cgccggtaac agcaacgagt tccccctcgc gcatcaggac atcaacaatt
      181 tccagctttt gcgtgggcga aattctcgca aacacatggc tcgaagtcac aagcttttca
      241 aaagccgggc tttctgtggg gcctgcctca ttcaactggg ctccggtcac caccggctca
      301 tcgatgtccg acaggcccag ttccctggct atggcaccgg cggtggcggg atgatccccg
      361 gtgatcatca gcaccttgat gccagcgctt ttgcatgtcc tcaccgcttc gatggcctcg
      421 ggacgcagag gatcgataaa ccctaccaat ccgtaaaaca acagcccaga aagaaacttg
      481 gcctgatcgg aggcttcgga tgggagactt tcagtttggc ccccggcaac ggccaacaca
      541 cggagacctt tttctgccat agcttgtgcc cgccgttcaa tatcgtcgcg gtcaagcggt
      601 aacggcgttc cgtcgcgaag gacgccactg caaaaatcga ggattgtttc cacggcaccc
      661 ttggcggcca cataggttgt gccatttttc tcgtagaaga ccgccgaata cttgtgctcc
      721 gactcatacg gaatctcgaa gaggatctta acttggcgtc ttgcctcggt cggactcaag
      781 cccaacttgt aacccatggc cagtaaggcg acatccatgg catccccgcg atgtttccag
      841 cccccttcct cgcgcacaag agacccttcg ttggccagaa ttgccgcttc ggccagccga
      901 gtcaactgct cgtggtcggt tggtgttatc tcgccgccct tccggaagac ctgtccttcg
      961 ccgctatagc cctctccggt aaactcgaac gtcctcccat ccgccaaatc aacgacgcga
     1021 acggtctgtt ggttgactgt cagcgtgcct gtcttatcac tggcgataac cgtgcagctt
     1081 cccaggcttt ccacagccgt gagcttgcgc acgatgacat tacgcgccga catccgtctc
     1141 gtggccacag aaagggccac agtcagggcc accggcagcc cctcgggtat ggcggaaact
     1201 gccagggcca ccacgaaaaa gaaaatcgac ggaatatcgt tgccctgaaa ccacagcagc
     1261 accgccaaaa aagtgctgat caccagcacc aggatggtga tttgcttgac gaaccgttcc
     1321 atccgcaaca ccagcggggg tttgcccatt tccgattcgg aaacagtttt ggcaataagg
     1381 cccacttgag tctcactgcc agtggcgaca acgattccga ctcccctgcc gctagtaatg
     1441 gtcgatcccg caaaggccat gttcttgcgg tccccaacaa cggtgtcctc gggcaggatg
     1501 tgggtggtct tttccgaggc gacggattcg cccgtcaaaa aactttcgtc ggccgctagg
     1561 ttgttgacct ctaccaaacg catgtcagcc ggtactttgt tgcccgactc gagcagaata
     1621 atatctcccg gcaccacctc ttccgcggga acttctattt ctttgccctt tctgcggacc
     1681 cgcgccgtga tggtgagcaa gcgtcgcaaa ccagcagc
//
LOCUS       DAOPTR010000926         1718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_54503_length_1718_cov_3.230908, whole genome shotgun sequence.
ACCESSION   DAOPTR010000926 DAOPTR010000000
VERSION     DAOPTR010000926.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54503_length_1718_cov_3.230908"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="PKI05_14565"
     CDS             <1..874
                     /locus_tag="PKI05_14565"
                     /inference="COORDINATES: protein motif:HMM:NF019305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53473.1"
                     /translation="DPEIAPLQYLLDFEVYCGLPLVGILYPRPPEKCDPIKGFSVWAD
                     EPAQLWWNDHQFQRILFRSAQRPFTIEEWPVLDYRGRITVALARDAVFGCYFPETLES
                     LERCGARIVDFSPVKDERIPEDTDVIYLGCGQPEPYAEDLANNHCLKAAIRNHVRRGG
                     RIYAEGAGAAYLCQWMEIQPGQFVRGVGLFPAAGRQLPRFGEPLPVTVTVARPMWLAS
                     RGTVLRGYRSPKWWFEPEPLLAPAIAEPGFEYDIIGNDRVVASVIHLDFGTHPDLLAS
                     FFAAKPESRLLPNP"
     gene            complement(882..1421)
                     /locus_tag="PKI05_14570"
     CDS             complement(882..1421)
                     /locus_tag="PKI05_14570"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOA53474.1"
                     /translation="MSSAAEIELKPFIRSIPDFPKPGILFRDITPLLASPQAMKATIR
                     YLSEAVRNRDVQMIAAAEARGFLFAAPLAIELNVGLVPIRKPGKLPSDTISHSYTLEY
                     GTDTLEMHKDAIAPGTRVLVVDDLLATGGTVEACCRLIEKAGGIVVGCAFVIELTDLK
                     GAERLARYHPVSLIKFSGK"
BASE COUNT          363 a          454 c          482 g          419 t
ORIGIN      
        1 cgatccagag atagcaccgc tccaatattt gctcgacttt gaagtttact gtggcttacc
       61 tttggtcgga attctctatc cgaggcctcc ggaaaaatgt gacccgatta agggattttc
      121 ggtttgggca gatgaaccag cgcagctttg gtggaatgac caccagtttc agaggattct
      181 ttttcggagt gctcaacggc catttaccat cgaggagtgg ccggtcttgg attatcgggg
      241 ccgcattacc gttgcccttg ccagggatgc tgtttttggg tgttattttc cggagacctt
      301 ggaatcgctg gaacgctgcg gggcacggat cgtggatttt tctccggtca aggacgaacg
      361 catcccggaa gataccgacg ttatctatct cgggtgtgga caaccggaac cttatgccga
      421 agatttggcg aacaatcact gcttgaaggc cgcgattcgg aaccatgttc gtcgtggggg
      481 aaggatttac gccgaaggag ccggagctgc ttatttgtgc cagtggatgg aaatccagcc
      541 ggggcagttt gtccgtggtg ttggtctctt tccggctgcc gggcgccaat tgccgcgatt
      601 tggtgagccg ctaccagtca cggtgaccgt agctcggcca atgtggttgg ccagcagggg
      661 gaccgttttg cggggttatc gaagccccaa atggtggttc gagccggagc cgcttttggc
      721 acccgcgata gccgaacctg gatttgagta cgacatcatc ggaaatgaca gagtcgtcgc
      781 ctccgtcatt catttggact ttgggacgca ccccgatctt ttggcgagtt tcttcgctgc
      841 caaacctgag tcacggctcc ttcccaatcc ctgatcatcc attactttcc actaaacttg
      901 atgagactga ccggatgata gcgagccaga cgttcggcac ccttgaggtc cgtcaactca
      961 atgacaaagg cacaccccac cacgatgcct cccgcctttt cgatcaatcg gcaacatgct
     1021 tcaactgttc ccccggtggc tagcaggtca tcaacaacga gcacccgggt acccggtgcg
     1081 atggcgtcct tatgcatctc caaggtatcg gtgccgtatt caagggtgta actgtgggaa
     1141 atagtatcag agggcaattt gcccggcttg cgaatgggta ccaatcccac attaagctcg
     1201 atcgccaaag gtgccgcaaa caaaaacccc ctcgcctctg ccgcggcgat catttggaca
     1261 tctcgattgc ggaccgcctc ggaaagatag cgaatggtcg ccttcatcgc ttgaggcgaa
     1321 gccaaaagcg gtgtgatgtc ccgaaacagg atccctggct ttggaaaatc gggaatgctg
     1381 cggataaatg gtttcagctc gatctcagcg gcagaactca ttaacgtggc tcctaatact
     1441 cctggctata cccatgcgtc attctcaact ttgtgattca aacccaccat tatggcgggg
     1501 aatccttttt gtgtaaacgc cgtggtcttc tcgggtgagt ggggcggttg gagagttagg
     1561 aatggggtca gcgaaaccat tgttggcctg attcggatct cttgacacgg tccatcttgc
     1621 ggcgcagtgt cactcgatga attcccaggg cgcgggcggc attcacatac tggccgccgt
     1681 agtgctgaat gacggtctcg aataacacgg gctccacg
//
LOCUS       DAOPTR010000927         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_54548_length_1717_cov_3.151625, whole genome shotgun sequence.
ACCESSION   DAOPTR010000927 DAOPTR010000000
VERSION     DAOPTR010000927.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54548_length_1717_cov_3.151625"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PKI05_14575"
     CDS             complement(<1..753)
                     /locus_tag="PKI05_14575"
                     /inference="COORDINATES: protein motif:HMM:NF037004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53475.1"
                     /translation="MNQALSLSRRFVSFLLWGGLLVALSTIPACGDPTGPRAQDRHIA
                     RTVALKLPQAHLTKHPLDQEIADRAFKLFMKSLDPMKLYFYQSDIDRFAAKKSQWVEL
                     LKAGDISPAYETYSVFLQRVDERVETALKILEQPLDFTVDEEIIRDPDLLTYPKTPEE
                     AYERWRKRIKLDLLVLKADKETTGQAAIEKLQKRYTSFCKRVHQTDEDELLETYLSAF
                     CEAYDPHTSYMSPETLENFDITMKLELEGIGAA"
     gene            complement(1011..>1717)
                     /locus_tag="PKI05_14580"
     CDS             complement(1011..>1717)
                     /locus_tag="PKI05_14580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOA53476.1"
                     /translation="VRRTIHHCRQSGIPPGICEVLENGVDCEFFRPQVNRDEKKEALG
                     IPPNRFVLGYVGRLSPEKNLTLLLDVARKLLAQNCEVHVLLVGDGPERRALSEHAHRI
                     GIADHITFAGYAADPRPYYAAMDVFLLLSRSEGLPNVLLEALATGLPVICTPVGGIPN
                     VIESGRNGLVVPLEDVPSPTAAIQQVMTDPKLARRLGEVGRQTVVSRYSFEHRMERLC
                     QSYWLLLGRDEMVETV"
BASE COUNT          405 a          480 c          456 g          376 t
ORIGIN      
        1 cgcagccccg atgccttcca gctcaagctt catggtgata tcgaagtttt cgagtgtttc
       61 cggggacatg tagctggtgt gcgggtcgta ggcctcgcaa aaggccgaga gatacgtttc
      121 cagcaactcg tcctcgtccg tttggtgaac acgtttgcag aagctggtgt aacgcttctg
      181 aagtttctca atcgcggcct ggcccgtcgt ttctttgtca gccttcaaaa cgagcaggtc
      241 gagcttgatc cgcttgcgcc aacgttcgta ggcttcctcc ggcgttttcg gataagtcaa
      301 cagatcagga tcgcggataa tctcctcatc cacggtgaag tccaacggtt gctcgagaat
      361 cttcaaggct gtctccaccc gttcgtcaac acgttggagg aacaccgagt aggtttcata
      421 agcgggactt atatcaccgg ctttcagaag ctcaacccac tgcgacttct tcgcagcgaa
      481 gcgatcgatg tcggactggt agaagtacag tttcatgggg tcgagcgact tcatgaaaag
      541 cttgaaggcc cggtcggcaa tctcctgatc caaaggatgc ttcgtgagat gagcctgggg
      601 aagtttgagg gccaccgtcc ttgcgatgtg ccggtcttgg gcacgagggc ccgtggggtc
      661 accgcaagcg ggaatcgtgc ttaaagcaac aagcaatcct ccccacaaga gaaacgacac
      721 aaaacgacga gacaaagaca gcgcttgatt catccttttc acccaaaatg tcaagagaaa
      781 cacccataat tgtcaatcgc tggttgctac ccggcgactg actttagcac acaatgacca
      841 tcatgcgacc gcaaggccac gcaaatccgg ccggtccacg ataatatttc cgaggcctct
      901 cctacttaac ccattgtatc cagttcgaga cctgtccgga aatgaacgcc tgtcggtgaa
      961 tcctaacggt tttacggaaa atgggcaaga tcggcgtgtt cgccgccaac tcatactgtc
     1021 tcgaccattt catctcgccc caacaaaagc cagtacgatt gacacaggcg ttccatacga
     1081 tgctcgaaac tgtaacggct gaccacggtt tgacgcccta cctcgccaag acgtctagca
     1141 agtttggggt cagtcatcac ctgctggatg gcggccgtcg gactggggac atcttccagg
     1201 ggaacaacga gaccattacg gcctgactcg atcacgttgg gaataccccc gacgggcgta
     1261 catatcacgg gtaaccctgt ggcaagggct tccagcaaca cattagggag gccttcgctt
     1321 cggcttaaca ggaggaatac atccattgcg gcatagtagg ggcgcggatc cgcagcatag
     1381 ccggcaaacg tgatgtggtc ggcgattcca attcgatgag catgctcgga caacgcccgg
     1441 cgctccgggc catcccccac gaggagaacg tgcacctcgc agttttgcgc gaggagcttt
     1501 cgagccacat ctagtaacag agtcaagttc ttttctggag ataaccgccc gacgtagccc
     1561 agcacaaacc gattgggagg aatgccaaga gcctcttttt tttcgtcacg attaacttgg
     1621 gggcggaaga attcgcaatc cacgccattt tccaggacct cgcaaatccc cgggggaatg
     1681 ccggactgtc ggcaatggtg tattgttctg cggacac
//
LOCUS       DAOPTR010000928         1716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_54599_length_1716_cov_3.001806, whole genome shotgun sequence.
ACCESSION   DAOPTR010000928 DAOPTR010000000
VERSION     DAOPTR010000928.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54599_length_1716_cov_3.001806"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="PKI05_14585"
     CDS             complement(<1..819)
                     /locus_tag="PKI05_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOA53477.1"
                     /translation="MLARPLPLQFAHHLPYGAILHEDGVQFVIFSRSATAMRILLYND
                     VNDREPSDIIHLNPADHRWGDVWNVFVPGLKAGQLYHIQADGPYAPEIGHRFDPKARL
                     IDPYAKALAGDFLPATDGIIRPPKCVVVDDTFDWQGTRHIRRDLSETIIYEMHVKGFT
                     RSPTSGAEFPGTYLGVIEKIPYLKSLGITAVELMPVHEFLILDCYGQKPKRPNYWGYD
                     PLAFFAPHRGYAVSKEPGAQVREFKEMVRELHKAGIEVILDVVFNHTAEGNEYGP"
BASE COUNT          412 a          431 c          473 g          400 t
ORIGIN      
        1 cggcccatat tcattgccct cggccgtgtg attgaacacg acatccagga tcacctcaat
       61 accggcctta tggagttcgc ggaccatctc cttaaattcg cgaacttggg cccctggttc
      121 tttactgacc gcgtatccgc gatgaggcgc gaagaacgcc agcgggtcgt agccccagta
      181 attgggccgc ttaggttttt gcccgtagca gtcgaggatc agaaactcgt ggaccggcat
      241 cagctcgacc gctgtgatgc cgagtgactt caagtagggg attttctcga tcacccccag
      301 atatgtcccc ggaaactctg ccccgctcgt aggagatcgt gtgaaccctt tgacgtgcat
      361 ttcgtaaatg atggtctccg acagatcgcg ccggatgtgc cgcgtaccct gccagtcaaa
      421 tgtgtcatcg acgacaacgc actttggagg acggataatg ccatcggtgg ccgggagaaa
      481 atcacccgcc agggccttgg cgtacgggtc gatcaatcgt gccttggggt caaaacggtg
      541 tccaatttcg ggagcataag ggccgtctgc ttggatgtgg taaagttgcc cagccttcaa
      601 tcccggcaca aaaacattcc acacgtcacc ccagcggtgg tctgccgggt tgagatgaat
      661 aatatcgctg ggttcacggt cgttgacatc gttatataaa aggatccgca ttgcggttgc
      721 agatcgacta aaaataacaa actggacgcc gtcttcatgc agaatagcgc cataaggcaa
      781 gtgatgggca aattgtaggg gtaatggtcg ggctaacatg gacagctgtt gcgcaggttg
      841 tcccacaaca aggtcaaagc ttccagcgta ccactgatgc aagtcggttg ccatggttca
      901 cccactattc ttgatattct aggccaaaaa acccgaaccg gaaaaacaaa tcgcccaact
      961 ttaaccgtcc gtggtgggag aagcgctatt tcgctcacct atagccgcca tccttgggga
     1021 tcggcctaac aacgaccact tagccccttc gtaatcccca acatcgggcg gcatcttcgg
     1081 gagcggcccg cgatccgtct tgggtgggaa cgatgaacga aagtgccgcg cttgggcgcg
     1141 acccttttcc ttccttatcg aaatcggacc ccgcccggcc tggcaacagt gttcctcagg
     1201 ctgggattcc tttcggcgca acctgaagac tttcctcaca ccgtccttgt catgagagaa
     1261 taggcaaact ctgtggattt aaacgatgcg cgattgaaaa agattgagga taaggccgac
     1321 ggtacggatc aatgccgctg gttgtatcat gccatccaaa cggaatgtga ctgccaggaa
     1381 ggacgcttgt cattggcgag atggttcccg acaggttggg aaaggtaatt gaaaggtgag
     1441 cggtttggtc gtcaggtcgc tccaaaagaa tcggaagact gttgggacga tacaaatcac
     1501 tctctggtcc cggttgagta gcggaccatg tggaactatt atactatgtt gtgtagttta
     1561 tcttgttggt tatgacagag cgtttattta aggccatcga tttgataaga acggtacaaa
     1621 ccgtgaacag ctttgtgggc taatagaagg gcgggaaaag accaattgag ggtggtgttt
     1681 tatgtggatc cttgcctgag tcgtagaagt agagga
//
LOCUS       DAOPTR010000929         1711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_54812_length_1711_cov_3.670290, whole genome shotgun sequence.
ACCESSION   DAOPTR010000929 DAOPTR010000000
VERSION     DAOPTR010000929.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54812_length_1711_cov_3.670290"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PKI05_14590"
     CDS             complement(<1..399)
                     /locus_tag="PKI05_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOA53478.1"
                     /translation="MTWTYFKRYRMEIALQQRELVEPPAPPGYSFLPWREDLLDIHAR
                     VKHLSFRGEIDAQIFPCFLDENSCRRLMLAIVRKPGFLPQATWLAVFSYQVEGKIYQE
                     YCGTIQGIYDAETRMGSIQNVGVIPGHRRSA"
     gene            complement(633..>1711)
                     /locus_tag="PKI05_14595"
     CDS             complement(633..>1711)
                     /locus_tag="PKI05_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53479.1"
                     /translation="WMSFRMAKRLYGRLGYPERMELAETDEKHGFSIQLREASVRWML
                     RWLAGRDEAVFEPSDLDVPTPAELQCTPQGEVMLLEGARSVYDLNRDYAKELAVRRQK
                     LWQSTPQEELINKVAQIVGLRRLAELPQPEVVACGTVRTEVCDVEKLIFRPEPGIELP
                     ALCFKPRDRKPSAGVIYIHEDGKAADAGPGGPIEKLIRDGKLVLAVDLRGLGETQRTG
                     QTYFRPQYHGPDGQDFYLAYHLEKSYVGMRAEDLLVVARWVKQERLPADVPLEVVSVG
                     YLNSVALHAAVRELGLFSHLTLVRPMMSWHNVVELGFSKNRLVNTVHGALEVYDLPDL
                     VRVLGDRVTIIDPLDAMGEPWKES"
BASE COUNT          382 a          542 c          443 g          344 t
ORIGIN      
        1 tgcggagcgc cggtggccgg gaatcacgcc cacattttga atcgacccca tcctcgtttc
       61 ggcatcgtag attccctgaa tcgtgccgca atactcctgg taaatcttcc cctcaacttg
      121 gtacgaaaag acggccaacc aagtggcctg gggcaaaaaa cccggtttgc ggacaatcgc
      181 cagcatcagc cgacgacaac tgttctcatc aagaaagcag ggaaaaattt gcgcgtcgat
      241 ctccccgcga aaactcaaat gcttgacccg ggcatggatg tcgagaaggt cttcgcgcca
      301 aggcagaaac gaatagccgg gcggggccgg cggctccacc aactctcgct gctggagagc
      361 gatctccata cgataacgtt tgaaatacgt ccaggtcatc ctgttcgcgg ggacattatg
      421 aacgcagccg gtacggccca caaagcctcg gaatactgcc ttaacctaaa tctaggctcc
      481 cccacttccc gggtcaacag tgtcccaaaa actgacttgg cccgtccttc tttagctaga
      541 tcacgcaaat aggcacccgc ggcatcccgt tgtggatcac gcgtcccgcg gcgctctcca
      601 tgcgcccagc ggctaatagg tcccgggctg gatcacgact ccttccaggg ttcacccatg
      661 gcgtcgagtg gatcgatgat cgtcacccga tcgccgagaa cccgaaccag atcgggcaaa
      721 tcgtaaacct ctagtgcccc gtgaaccgta tttaccaagc gatttttact aaagcccagc
      781 tcgaccacat tgtgccaaga catcatcggt cgtactagcg tgagatggct gaaaagcccc
      841 agctctctta ccgccgcatg cagtgccacg ctattcaaat agccgaccga caccacttcc
      901 aagggaacat cggcgggtaa ccgctcctgt ttaacccagc gtgccacgac tagcagatct
      961 tccgcccgca tgcccacata ggacttttcc aggtgataag cgagataaaa atcctggcca
     1021 tcggggccgt gatattgtgg gcggaaatag gtttggcccg tccgctgcgt ctcgcccaat
     1081 ccacgaagat cgaccgccag gacgagtttg ccatcccgaa tgagcttttc gatgggccct
     1141 cccggaccgg cgtcggctgc cttgccatcc tcgtggatgt agatgacgcc cgcggatggc
     1201 tttcgatcgc gcggcttgaa acagagggcg ggcaattcaa tccccggttc aggacgaaaa
     1261 atgagctttt ccacgtcgca aacttcggtg cgaactgttc cacaagccac gacctccggc
     1321 tggggtaact cggccagtcg ccgcaagccc acaatctggg caaccttgtt gatcaattct
     1381 tcctgcggcg tgctttgcca cagtttctga cgtcgcaccg ccaactcttt ggcgtaatca
     1441 cggttgagat cgtaaaccga gcgggcccct tctaggagca tgacctcgcc ttgtggcgtg
     1501 cattggagct ccgccggcgt tgggacatcc agatcggaag gttcaaagac cgcctcatct
     1561 cgccccgcca gccaacggag catccaccgc acagaggcct cacgcagttg aatgctgaag
     1621 ccgtgcttct cgtctgtctc ggcaagttcc attcgttccg gataccccag gcgaccataa
     1681 agccgcttcg ccatgcggaa ggacatccag g
//
LOCUS       DAOPTR010000930         1710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_54847_length_1710_cov_3.985498, whole genome shotgun sequence.
ACCESSION   DAOPTR010000930 DAOPTR010000000
VERSION     DAOPTR010000930.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54847_length_1710_cov_3.985498"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PKI05_14600"
     CDS             complement(<1..417)
                     /locus_tag="PKI05_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOA53480.1"
                     /translation="MDSTSFTQQIPALVVTGLGLATAVAPCPLATNIAALSYIGRKVV
                     DRRWVLLSGILYTAGRSFTYFALAAVLVTGLTNLPGLAMTLQYYGHLFLGPILILIGM
                     MLLGLIVIPLPGAKSEQAHALADRYGLIGALLIGVLF"
     gene            complement(477..953)
                     /locus_tag="PKI05_14605"
     CDS             complement(477..953)
                     /locus_tag="PKI05_14605"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HOA53481.1"
                     /translation="MKMCSKLVLFLATLVTLGSAGAILGQTDTQTAPAAIPPNRIIAA
                     YFHRTQRCPTCLRVSQTVANILKAHFAEQMKSNQVQFVEIDFQAPQNAHLARAYQITG
                     PQLVLIQVEGGRVVAWQPMPKVWSLLIKPQELDQYVTSNVEAYLHQLGHAQASVVR"
     gene            complement(1017..1541)
                     /locus_tag="PKI05_14610"
     CDS             complement(1017..1541)
                     /locus_tag="PKI05_14610"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HOA53482.1"
                     /translation="MNPNNSWQTLLTGALLLLVGAAFGAIAVKLSSRESPGSQGGSIT
                     AAAQTTSNAQGSPTAFRDGVFVYLFHGNYRCPTCLKIEASTKEVLENKFADAIRSGKV
                     VVKELNYEQPENRGYIQKYQLIAPTVVMVQVRNGQEIRFENLMDVWQLVGEPEKFAEF
                     IESNLQKFLTEETT"
BASE COUNT          425 a          451 c          445 g          389 t
ORIGIN      
        1 gaacaaaaca ccgatcaaca acgcgccgat caaaccataa cgatcggcca aggcatgcgc
       61 ttgctccgat ttggcccccg gcaagggaat gacaatcaat cctaagagca tcatcccaat
      121 gaggatcagg atcgggccaa ggaaaagatg accatagtac tgaagggtca ttgccaaccc
      181 tggaaggttg gtcagtcctg ttactaggac agcagctagt gcaaaatagg taaacgatcg
      241 cccggccgtg tagagaatgc cggaaagaag gacccagcga cgatccacca cttttcggcc
      301 aatgtacgat aaggcagcga tattggtggc aaggggacag ggcgccaccg cagtggccag
      361 cccaagcccc gtgacaacga gggccggtat ttgctgtgtg aagctcgtgg agtccatcgt
      421 cggctatttc cctaattcac tcaagataga tgttcaaaag ggtcgcaaga ccacactcag
      481 cggaccacac tggcctgtgc gtgccctaac tgatgaaggt aagcctcaac gttgctggtt
      541 acgtactgat ccaattcttg cggtttgatg agcaaactcc acaccttcgg catgggctgc
      601 cacgccacca cgcgaccacc ttcgacctga atcaagacga gttgcggccc ggtgatctgg
      661 tacgccctgg cgagatgggc attttgggga gcctgaaagt caatctcaac aaactgaacc
      721 tggtttgact tcatctgctc ggcaaagtgg gctttcaaga tattggccac cgtttgcgag
      781 acccgcaaac atgtgggaca acgctgcgtt cgatggaaat acgcagcaat gatccgattc
      841 ggcggaatcg cggccggcgc cgtttgcgtg tcggtttggc ccagtatcgc accggcacta
      901 cccaaggtaa ccagcgttgc gagaaaaagg acaagttttg aacacatctt catcggttat
      961 cctccgtgaa atgatatgac ctggatacgc gttggtgggt gaacacgcag aaacgctcac
     1021 gttgtctctt cggttaagaa cttctgtagg ttactctcaa taaactcggc gaacttttcc
     1081 ggttcgccaa cgagttgcca gacgtccatg agattttcaa agcggatttc ttggccattt
     1141 cgaacttgga ccatgacgac cgtaggtgcg atgagttggt acttctggat gtaaccgcga
     1201 ttctcaggtt gctcataatt caattctttc acaaccactt ttcctgagcg aatagcgtcg
     1261 gcgaatttgt tttccaggac ctctttggtg cttgcctcga ttttcagaca cgtcggacaa
     1321 cgataatttc catggaacag atacacaaag acaccatcgc ggaaggccgt gggggatcct
     1381 tgcgcatttg atgtcgtttg cgccgctgcg gtaatggagc cgccttgact gcctggcgat
     1441 tctcgacttg atagtttcac cgcgatggca ccaaaagcag cacctaccaa tagcagcagt
     1501 gcgccagtta aaagtgtttg ccacgagttg tttgggttca tgaggaacct cctcttctgt
     1561 gatcatgact cgacgaaaat gcaatccaaa cgccgaggat gcgaccacat ccccggttcg
     1621 attgcggttc acaaaaggtt atcggcaaca cgcatccgtg gaatcttgtg ccgtcgttcc
     1681 gcataatgtg ccaatttcct ggcaaacggc
//
LOCUS       DAOPTR010000931         1709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_54907_length_1709_cov_3.218863, whole genome shotgun sequence.
ACCESSION   DAOPTR010000931 DAOPTR010000000
VERSION     DAOPTR010000931.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54907_length_1709_cov_3.218863"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="PKI05_14615"
     CDS             <1..928
                     /locus_tag="PKI05_14615"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOA53483.1"
                     /translation="GSGKSTDTFRWDETSEVTLTLRDDHGNPVLAYNFGPITNPSVPA
                     NDPRRTRACYIHPVWGLKGEVLTDDFPRDHYHHHGIFWTWPHVVIRDQEYSLWEDKGA
                     LRQRFRKWLCREAGEHAAVLGVENGWMIDEECLLAERVWITVYKADSLGRMIDIDLFL
                     LPHVPVTLWGAEGKSYGGLTMRFRPPSQKDAMITVPAGLTGTDLYETRLPWADFTSLF
                     GDDGASLSGGTIMVAPDHPDYPPTWLTRHYGPLCIGWPGIQSRTLEPGSCTRLSYRIW
                     IHSDLRTAAEIHKIYEMFCAAAKLVCQKSDSQ"
     gene            complement(1043..>1709)
                     /locus_tag="PKI05_14620"
     CDS             complement(1043..>1709)
                     /locus_tag="PKI05_14620"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOA53484.1"
                     /translation="ALKGWWLSSTHLPEDNMATILEPFELEALDSLPFDSAAYITPSE
                     STIASLVRRAKDGDNEAWEQLIQRYQKLVYDTVFAKLRSHSDAEEVCQEVFLQALRKL
                     FQLEVPEAFGSWLKRIAERLAINRLSRGRSEYALTDTQMERQRDLRAEPVREVMRREA
                     QQRVLCRLNDLRSMDREALVAFYLEGKSLAEISHELAVPVGTVKRRLHVARKRLAKLL
                     EQE"
BASE COUNT          368 a          481 c          484 g          376 t
ORIGIN      
        1 aggctccggc aagtcaactg acacatttcg ctgggacgag acctcggagg taacgctcac
       61 cctccgtgac gatcatggta acccagtgct agcgtacaac tttgggccca tcacgaatcc
      121 ttccgtcccg gctaacgatc cgcgtcggac gcgggcatgc tatattcatc cggtgtgggg
      181 acttaagggc gaagtcctta cagacgactt tccacgtgat cattaccatc atcatggcat
      241 tttctggacc tggccccacg tggtgatccg cgatcaggag tatagtctct gggaagacaa
      301 aggagcattg cgacaacgat ttcgcaagtg gctttgccgc gaagccggtg agcatgccgc
      361 ggtgctgggc gtggaaaatg gctggatgat cgacgaagag tgtttgctcg cggaacgggt
      421 gtggatcaca gtgtataaag cggattcgct ggggaggatg atcgacatcg acctgtttct
      481 cctcccccat gtcccggtca cgctttgggg tgccgagggc aagagctacg gcggtctcac
      541 gatgcggttt cgaccgcctt cacaaaaaga tgctatgatt acagtccccg caggcttaac
      601 gggcactgac ctttatgaaa cacgcttgcc ctgggccgac ttcacaagcc tttttggtga
      661 cgatggggcc agtctaagcg ggggaaccat catggtggca cccgaccatc ccgattaccc
      721 gcctacttgg ctcacccgcc attacggccc gctttgcatc ggttggccgg ggattcagag
      781 tcgaacactc gagccgggaa gctgcacgcg gctgtcttac cggatttgga tccattctga
      841 tcttcgcacg gcagccgaaa ttcacaagat ttacgagatg ttctgtgccg ccgcaaaact
      901 tgtatgccag aagtcagact cacagtgagg atgacacaga ccctttgacc cgcaaggcga
      961 caacggtatg cgtcggcttg ggtgataaac ccgcttggcg ccgtgagcgt tccacgaaca
     1021 gaggccggct gagtgtacaa tatcactcct gctcaagcag tttggccagc cgttttcgtg
     1081 ccacgtgcag ccgtcgtttg accgtaccca cgggaactgc aagttcgtga ctgatttccg
     1141 cgagtgattt accctcaagg tagaaggcga caagcgcctc gcggtccatc gatcggaggt
     1201 cattcagacg gcagagaacc cgttgctgag cctctcgtcg catgacttcc cgaacgggtt
     1261 cggcccgaag gtctcgttgc ctctccattt gggtgtccgt cagtgcatat tccgatcgac
     1321 cgcggctgag acgattaata gccaagcgct ccgcgattcg tttcagccaa ctcccaaagg
     1381 cctccgggac ttccagttga aacagtttgc gaagggcttg caggaagacc tcctggcaca
     1441 cctcttcggc atcggagtgg ctccgcagct tcgcaaagac ggtgtcgtaa accagctttt
     1501 gataacgctg gatcaactgt tcccaagcct cgttatcacc atccttcgca cgccgaacca
     1561 aggaggcgat agtgctttcg gacggggtaa tataggctgc ggagtcgaaa ggtaggctat
     1621 cgagcgcttc gagttcaaag ggttcgagaa ttgtagccat gttatcctcc ggcaaatggg
     1681 tggatgagag ccaccaccct ttgagagcc
//
LOCUS       DAOPTR010000932         1708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_54963_length_1708_cov_2.258318, whole genome shotgun sequence.
ACCESSION   DAOPTR010000932 DAOPTR010000000
VERSION     DAOPTR010000932.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54963_length_1708_cov_2.258318"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            355..777
                     /locus_tag="PKI05_14625"
     CDS             355..777
                     /locus_tag="PKI05_14625"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOA53485.1"
                     /translation="MDENNVFASRLRKLRKEVGLSQEEFAKRLGFSIDAVGSWERGIR
                     NPASRALGRIAQYFGVDPRYLTGETANRYERPDETLAVWLDDQRGNDERYATMMSELS
                     DAGKLRVYQTIVSTHFADEESGELRDPGYVMSAKDKNA"
     gene            1216..>1708
                     /locus_tag="PKI05_14630"
     CDS             1216..>1708
                     /locus_tag="PKI05_14630"
                     /inference="COORDINATES: protein motif:HMM:NF036822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53486.1"
                     /translation="MKLLILGAGEYGQLVKEQARNKYTTIDFLDDNSSVAIGKFEEYK
                     KFVGEYEAIVAIGNNEARLTWIEKLVEAGFEIPTLISPQAFISPSAKIEMGCIVEPMA
                     VINANAIVEKGTIVSAGAVVNHNAVVRQGCHIDCNAVVGADAVVPEKMHLNYGQVITR
                     VTKP"
BASE COUNT          517 a          361 c          466 g          364 t
ORIGIN      
        1 ggcgacacag gcccgcgatc cttttccgtg gtaccaacat agtgaaattc gatgaaaatc
       61 cgtacaaatg atgtaacgcg tacgttacat ttgcacaacg gtacgttaca tttcggaccc
      121 atatcagcat gatagagttg aatgaagatg ggggcctgga aataatgggt gaagaaatgg
      181 tcgcttgcac ctttccaaaa cagtggaatc cgaatcatct ccaaggcgat tcacgccgtt
      241 agaagacgaa aacggcgaac cacgggaagc cctcactgca atacatgcaa ttcgtgtccg
      301 acggacggac cgcggaaccg cttaacagta acgagataag gaggcacgca aaagatggat
      361 gagaataatg tttttgcctc tagacttagg aagctccgca aggaggtggg cttgagccag
      421 gaggagttcg cgaagaggct cggcttctcg atagacgccg tcggctcctg ggagaggggg
      481 ataagaaacc ccgccagcag ggccttgggt aggatagctc agtacttcgg cgtggatccc
      541 cgttacctaa cgggcgagac ggcgaatagg tacgagcgcc cggacgagac gctggccgtc
      601 tggctcgacg atcaaagggg caacgacgag aggtacgcga ccatgatgag cgagctctcc
      661 gacgccggca agctcagggt gtaccagacg attgtgtcca cgcacttcgc cgacgaggag
      721 tcgggcgaat tgagggaccc gggctacgtg atgagtgcca aagacaaaaa tgcatgacga
      781 ttcgtgcaaa tcatgtcgaa tctgtacgga aagcgccgaa acaaggatat ttatatggat
      841 aaacatgggg atttgcgtga aaatcgcgtc ggaaatgtgt atctgacgga cctcaaaatc
      901 cgtacaaatg gctgtttagc gatcgaaaaa accgtacaaa acacacaaaa atgcctaaaa
      961 tccgtacaaa aatcctcaat tcgatgtcca ttgttgcaaa gcgtgtaacg cgcgcaaaac
     1021 ttttgcacaa taggacgtta cattttagcc ctgttttcgg gtggtaaatc tgaatgaata
     1081 tgagggttga aggcgctgac ttttgaagaa atgataaaat agtcgcgcct tgtcggatcc
     1141 gctacaaata gtgaacaaaa caagaagccc tcatggaaat gaatgtaggg cgagatagca
     1201 ataggaaagg ctcatatgaa acttttgata ttgggtgccg gtgaatatgg gcagttagtc
     1261 aaagaacagg cacgaaacaa atatacgacg atagattttc tcgacgacaa ctcaagtgtt
     1321 gcaattggaa aatttgaaga atacaagaag ttcgttggcg aatacgaagc aatcgtggct
     1381 attgggaaca atgaagcgag acttacttgg atcgaaaaat tggttgaagc gggatttgaa
     1441 attccaactt tgatcagtcc gcaagcgttc atttctccat cagcaaagat agaaatgggt
     1501 tgcattgttg agccgatggc ggtaatcaat gccaatgcca tcgtcgagaa agggacgatc
     1561 gtatctgctg gagcggtagt taatcataat gcggtagtca gacaaggctg ccacatcgat
     1621 tgcaacgctg tggtcggtgc tgatgccgta gtacccgaaa agatgcattt aaactatggc
     1681 caagtgatca caagggtcac gaaacctg
//
LOCUS       DAOPTR010000933         1707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_54991_length_1707_cov_4.019976, whole genome shotgun sequence.
ACCESSION   DAOPTR010000933 DAOPTR010000000
VERSION     DAOPTR010000933.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54991_length_1707_cov_4.019976"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..>1707
                     /locus_tag="PKI05_14635"
     CDS             29..>1707
                     /locus_tag="PKI05_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53487.1"
                     /translation="MATASSLDGPWTQRKVAVPHGGQSTMFEGPEGQWYATFSGVDSR
                     AALRERPAIVPVDWVDSVMYFYPKNEPWPWKRPEVITEAWGWENARPLSDLRYRDPYG
                     VNGRDGYFYVSGLHSPGSHGRRVELLRGKDLTGGEPWEPFPIPGFETLDRVPWFNEKA
                     EANFDVSLCKPFYAAGTFWINLWVRGGKRLLRSESGTMQGPWKVAVDPPPSPSAPVGY
                     WCAHPFQDHRGDMYGYLYATLWPMNSTFTQLEADRKPPVEDGYEPMFDRRLRGYVRES
                     SDGSHLLRGDAPIGHVFWIDGHYLMMGGCDWHGEYRRFGTYDSAVYWAHEIGGPWHPN
                     RSVLPHCGNSGLFQDDNGTWWVVSFANDNFLPDHSRLRCLPLEIKWNGNGYDIRPKHK
                     EANPYVHREEAIVPDAQAERIAPAYPVIKLPEDIILQSPSITLVKEEGSAVYYLTGTA
                     AVETATGLKPGSRTSKPDFHNNDGIYLWRSTDLKTWVALGKVFDLATVNRKASKASGW
                     NSPFGVYFTPPDSLEPRYDRAVVTPKLYRIRNDYWIVCSCGRQQVCLLKSQ"
BASE COUNT          372 a          468 c          486 g          381 t
ORIGIN      
        1 cttgggtgta ccgtgctacg atacgtttgt ggccactgcc tcgtcgcttg acggcccttg
       61 gacccagcgc aaagttgccg tcccgcatgg cggtcaatcc acgatgtttg agggaccaga
      121 ggggcagtgg tatgcgacct tcagcggagt ggacagccgg gcggccttgc gcgagcggcc
      181 ggccattgtc ccagtggact gggtcgacag tgttatgtac ttctacccga aaaatgagcc
      241 ttggccatgg aaaagaccag aagtgattac ggaagcgtgg ggttgggaaa acgcccgccc
      301 actcagtgac ctcaggtacc gagatcccta cggtgtaaat ggtcgcgacg ggtatttcta
      361 tgtttcgggt ttgcacagtc ccggttcgca tgggcgccgc gtggaattgt tgcgaggaaa
      421 ggacctgacc ggcggcgaac cgtgggagcc gtttccgatc cccggttttg agacactcga
      481 ccgggtcccg tggttcaacg aaaaagccga ggctaacttc gatgtttctt tgtgtaaacc
      541 gttttatgct gccggtacgt tttggatcaa tctttgggtt cgagggggca aacggctcct
      601 ccgcagcgag tctggcacaa tgcaaggccc ttggaaggtt gcggttgatc cccctccctc
      661 tccatccgcc cctgttggct attggtgtgc ccatcccttc caagatcaca ggggagacat
      721 gtacggctac ctttacgcga ctctgtggcc catgaacagt acgttcaccc aacttgaggc
      781 cgatcgaaaa ccacctgtcg aagatggata tgaacccatg tttgaccgac ggctccgggg
      841 ttacgttcgc gaatcctcgg acggatctca cctattgcgt ggtgacgcgc cgatcggcca
      901 cgttttttgg atcgatggcc attacttaat gatgggcggc tgtgactggc acggggaata
      961 ccgccgcttc gggacttatg acagtgccgt ctattgggcg cacgaaatcg gtggcccatg
     1021 gcatcccaac cgctcagtat tgccacattg cggcaactcc gggctgtttc aagatgataa
     1081 cggaacctgg tgggttgtca gctttgccaa cgacaatttt ctgcctgacc attcaaggct
     1141 gcgctgcctt ccattggaaa ttaaatggaa cggcaatgga tacgacatta gaccgaagca
     1201 caaggaagcc aatccttacg tccatcgcga agaggcgatc gttcccgacg cccaggcaga
     1261 gagaatcgcg ccggcatatc cggtaataaa attgccagaa gacattatcc tccagtcacc
     1321 gtcgattacc ctcgtgaagg aagaagggtc agcggtgtac tatctcacgg gaacggcggc
     1381 ggtcgaaacc gcaacagggc tgaaaccagg gtcacgaact tcgaaacccg attttcacaa
     1441 taacgacggc atttaccttt ggcgatccac agacctgaaa acatgggttg cgctcggcaa
     1501 ggtgttcgac ctagctacgg tcaatcgtaa agcctcaaaa gcgtcgggct ggaatagtcc
     1561 gttcggcgtc tatttcaccc cgcccgatag cttggagccc cgctacgacc gtgccgtggt
     1621 aacccccaag ctttaccgca tccgtaatga ctattggatt gtctgttcct gcggccggca
     1681 acaggtgtgt ttgctgaaaa gtcaaag
//
LOCUS       DAOPTR010000934         1707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_55000_length_1707_cov_3.214286, whole genome shotgun sequence.
ACCESSION   DAOPTR010000934 DAOPTR010000000
VERSION     DAOPTR010000934.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55000_length_1707_cov_3.214286"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            421..1266
                     /locus_tag="PKI05_14640"
     CDS             421..1266
                     /locus_tag="PKI05_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOA53488.1"
                     /translation="MEKWPIGVFASIDAGLGVQLSVAHELGVPTIQLHTPKKSSRTPE
                     NAKRFLQQLQEYGLTITVVFAGFEGESYADIPTVAKTVGLVPPETREERKQELKEIAD
                     FAKLLGVDAVGLHIGFIPHDRQDPLYQDVLRVAQEVCDYCANQGQRIHLETGQESADT
                     LLQFLKDVNRSNLFVNFDPANMILYGSGDPIEALKKVGAYVRSVHCKDAKWAAQPGKE
                     WGVEVPFGTGDVNAELFLKTLAELGYDGALTIEREIPQEPERQKQEIGHAIRVIQEIK
                     RKIFG"
     gene            1310..>1707
                     /locus_tag="PKI05_14645"
     CDS             1310..>1707
                     /locus_tag="PKI05_14645"
                     /inference="COORDINATES: protein motif:HMM:NF013495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOA53489.1"
                     /translation="MITRICGVLVQITEETATLEAGSFWWEVLIPEYTRRQLQSRINQ
                     EVVLHTTLYLEGAVNTGRLTPRLVGFINEIEREFFDLLCTVDGMGAKKALRAMSHPVN
                     EIANAIEQQDHRFLASLPGVGPATAERIVA"
BASE COUNT          399 a          450 c          488 g          370 t
ORIGIN      
        1 actcgtctcc gcaaacgaaa attggtgttt ctcccaccca gaacgctaac acagttacgg
       61 ccagccacca tcgtgcagga tcagctctca tggaaaagac tcctgtggtt cttagcttga
      121 gctgatcgac ccgtctggac ccatgccgag aaaaactcac ccttctcaaa gctccccgca
      181 actcgagacc gcttgcccat actcatgaat tgccaaaact tgccagcgcg caccaagggc
      241 cgacgatgcg acccctccgg tggggtcggc cgttgcttgc agttggtggg cttctgcggt
      301 atcttgacta caacgcttgt tttacgcggg aatcgtttgg ggaagtccag ggcaacgcgg
      361 agccgtcatc ggccgcgact gtcgctggcc aagggtcgaa aggtcgtgga ggactcagct
      421 gtggaaaaat ggccaatcgg tgtttttgcc agtattgacg caggattagg cgtgcaattg
      481 agcgttgctc acgaattggg ggtacccacc atccagttgc acacgccgaa gaaaagttct
      541 cggacgcccg agaatgccaa acgctttttg cagcaattgc aagaatacgg actgaccatt
      601 acggtcgttt tcgccggctt cgagggtgaa agctacgcgg atattccaac ggtggcaaag
      661 accgttggcc ttgtcccgcc ggaaacccgc gaggagcgta agcaggaact caaagaaatc
      721 gcggactttg caaagctcct tggcgttgat gccgtgggtc tccacatcgg gttcattccc
      781 catgaccggc aggatccgct ttatcaggat gttttgaggg tagctcagga ggtctgcgat
      841 tactgtgcaa accaggggca gcggatccat ctggagacgg gccaagaatc cgccgatacg
      901 ttgctgcaat tcctaaagga tgtgaatcgg agcaacctgt tcgtcaactt tgatcctgcc
      961 aacatgatcc tttacgggtc cggtgatccg atcgaggccc tcaaaaaggt cggggcttac
     1021 gtgcgaagtg tccactgcaa ggatgccaaa tgggctgccc aacccggtaa agagtgggga
     1081 gtcgaagtgc ctttcggcac cggggatgta aatgccgagc tgttcttgaa gaccttggcc
     1141 gaactgggtt acgacggtgc cttaaccatt gaacgggaga ttccgcagga accagaacgg
     1201 caaaaacagg agatcggcca cgctattcgc gtcattcaag agatcaaacg gaagattttc
     1261 ggttaacgga cgatcgaatc cttgagttat taggaaggac gttccaccct tgatcacgcg
     1321 catttgcggt gtattggttc aaatcacgga ggaaaccgca acactcgaag ctggttcgtt
     1381 ctggtgggag gttttaatcc ccgaatacac acggcgacaa cttcaatcgc gcatcaatca
     1441 ggaggttgtc ttacatacga ccctctatct ggagggtgcg gtcaacacgg gtcgattaac
     1501 gccgcggttg gtaggtttta tcaacgagat cgagcgcgag ttttttgact tgctatgcac
     1561 cgtcgatggc atgggggcta agaaggccct gcgtgccatg tcgcaccctg tcaatgaaat
     1621 cgccaacgcg atcgagcagc aggatcaccg ctttcttgcg agcctgccgg gagtggggcc
     1681 ggccacagca gagcggattg tggccaa
//
LOCUS       DAOPTR010000935         1706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_55053_length_1706_cov_3.086614, whole genome shotgun sequence.
ACCESSION   DAOPTR010000935 DAOPTR010000000
VERSION     DAOPTR010000935.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55053_length_1706_cov_3.086614"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            338..1315
                     /locus_tag="PKI05_14650"
     CDS             338..1315
                     /locus_tag="PKI05_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53490.1"
                     /translation="MSSDNLTVTCPHCGRQSRMPPGITGRTVRCPYCKETWKVPGGSQ
                     QASSISSSGPAGGVSGAERGGSPYEVSDPFALLSQTGGDVTAPVESTFSASTAPGMAT
                     APRTSAPPAWPMWWFLLGGLSVLLLISVVVAIVVVVVLFLQRSPQPAPPSQPPPVIAE
                     PAPPQPNPEDELARKGAATAAFLEAVFNESRKVLGDFSNLTNLGAGGASLPPDTPRRL
                     AAMEQALARLDTTDVDPVALQFKDRYVSEFKRLLNTMATTSQNLNAALQGGNPEAVLN
                     MAEQLGVAQSEYLKATSSFFEFLNTEGRRVKTDLESRYHRSFPMPLVPQ"
     gene            1433..>1706
                     /locus_tag="PKI05_14655"
     CDS             1433..>1706
                     /locus_tag="PKI05_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53491.1"
                     /translation="MKRMRSCGGFLLLSVGLFFYAVLGPMVLGAEYYVDPQNGKPNND
                     GSKERPWPSLQALFDQGAIETQTWESLPYKPGVKLVPKNAGATIRPG"
BASE COUNT          362 a          420 c          507 g          417 t
ORIGIN      
        1 acggttctcc tttgtccgtc accaggtaac ggccattggg gctaactttg aggagcggtg
       61 gtgccgcgtc agagttggat accaagcctt gtaatacggc gcctaaaact aataggctta
      121 cgagaaagcg gctcattaat gttctcccaa caaaacgcat ttgaatcggt gggtggcagt
      181 ttcaagacgt ttttctttgt ctattttagc gttagctcct gggcatacta gaatggccag
      241 gggatccctt gcagatggtg caaacgcctc cgcctcacac tatgatggat tgtcggctga
      301 gatgatggca ttcaaccaaa actttggggg tttgggtatg tcttctgaca atttgacggt
      361 gacttgtccc cactgtggcc gacagtcgcg gatgccgccg ggcatcacag ggcgaacggt
      421 acgttgcccc tattgcaaag agacatggaa agtgccggga gggtcccagc aggcctcttc
      481 gatcagctca tctggacctg ctggaggagt ttccggagcc gaaagggggg gttctccata
      541 tgaagtgtcc gatcctttcg cgctattgag ccaaacgggg ggagatgtaa cggctccggt
      601 tgagagcacg ttttctgcca gcacggctcc ggggatggcc accgcaccgc ggaccagtgc
      661 ccctccggct tggccgatgt ggtggtttct gctgggcggt ctctccgttc ttctgttgat
      721 tagcgtggtg gttgctatcg tcgttgtggt tgtattattt ctgcaaagat cgccgcagcc
      781 ggccccgcct tcgcagcctc cgccggtgat agcggaacct gcacccccgc agccgaatcc
      841 ggaagacgag ttggctcgca aaggtgcagc cactgcggca tttctggagg cggttttcaa
      901 cgaaagccgc aaggtgcttg gtgatttctc gaacctaacc aacttaggtg ctggcggagc
      961 tagcctgccg ccggatactc cgcggagact ggccgctatg gaacaagcct tggcccggct
     1021 tgataccacg gacgtcgatc ccgtcgccct tcaattcaaa gatcgttatg tgagcgaatt
     1081 taagagactc ctgaatacga tggccacaac cagccaaaac ttgaatgcgg ctctccaagg
     1141 gggtaatccg gaggccgttt tgaacatggc cgagcagtta ggtgtggctc agagtgagta
     1201 tctcaaagcc acaagtagtt tcttcgagtt tctcaatacc gaaggtcgaa gggtcaagac
     1261 ggatttggag agccgctacc atcgcagttt cccgatgccg ttggtacctc agtgaaaaga
     1321 cttgtgccgc ggtctggtta atcattgccg attgcggaca accgacttag ggatagggaa
     1381 ctcgtgctat tttgagtggc tttggcgtga tgttgaatgc gaggatttaa gaatgaaaag
     1441 aatgcgtagt tgtggcggtt ttctgttatt gtctgttggg ttatttttct acgctgtttt
     1501 gggtccgatg gtcctggggg ctgagtacta cgtcgatccg cagaatggaa aaccaaacaa
     1561 tgacggaagc aaggagcggc cttggccgag tcttcaagcg ttgtttgacc agggtgcgat
     1621 cgaaacgcaa acctgggaat ccctgcctta caaacctggt gttaagttgg ttcccaaaaa
     1681 tgcgggggca acgattcggc cgggcg
//
LOCUS       DAOPTR010000936         1705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_55107_length_1705_cov_2.635152, whole genome shotgun sequence.
ACCESSION   DAOPTR010000936 DAOPTR010000000
VERSION     DAOPTR010000936.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55107_length_1705_cov_2.635152"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PKI05_14660"
     CDS             <1..417
                     /locus_tag="PKI05_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53492.1"
                     /translation="GHESRAPFMRDIVLTDLEIIHFSMTPFLLEPGEDMRLENVLVLG
                     VRIHGEGQQELIRLRPVVNQYMRKKTPGFIKNVTFKDVTVYGAPGGYRVQIHGADAEH
                     NVRDVTFDNVVILDEKLTGESSRLETGPHVEGVTFK"
     gene            complement(558..>1705)
                     /locus_tag="PKI05_14665"
     CDS             complement(558..>1705)
                     /locus_tag="PKI05_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53493.1"
                     /translation="REKVVRLKNDLDPLKIFLCATHTHTAPVTVEGKYTLPETGVMRP
                     SAYTDWMTTQIAQAIGQAWEKRQPAQVGWGQGQAVVAQNRRAVYADGSAKMYGATNVP
                     EFRGMESQEDHNLEVLFFWNNEGKLIATAINIACPAQEVEGLSVIHADFWHPVRELLR
                     SRYGSDLAILGLTGAGGDVTPHLMYGKAADERMRKLRNLSRLDEIARRIVRGWEEAYE
                     VAKLDMRKDVVLKHVVQTIELPYRRVTEAEAEEARKLAAEYADKPSERWNYLWHQRVV
                     DLYEKQQSGEIGSLPMELHVIRLGDIAIATNPFELYTDYGIQIKARSPAVQTFVVQLA
                     VGSGGYLPTARAVQGGGYGAVIQSGRIGPEGGQVLVDRTVEAISQLW"
BASE COUNT          368 a          493 c          449 g          395 t
ORIGIN      
        1 ggtcatgaaa gtcgggcacc cttcatgcgc gatattgtct tgaccgatct ggagattatc
       61 catttctcga tgacgccttt tctcttggaa ccgggtgaag acatgcgttt ggagaatgtc
      121 ctcgtcttgg gtgtccggat acatggtgaa ggccagcagg agctcattcg cctacgtccc
      181 gtagttaacc agtacatgag aaaaaagaca ccaggtttta ttaagaatgt cacatttaag
      241 gatgtaacgg tgtatggtgc ccctgggggc taccgcgtgc aaatccacgg agcggatgcc
      301 gagcataatg tgcgtgacgt gacatttgat aacgtcgtca ttctggacga gaagcttacg
      361 ggcgaatcca gtcgattgga aaccggccca cacgtggaag gcgtgacatt taagtagccc
      421 cgcacgtgga catcgccaag tagctctgtt tggggtttcg aggacggccc tctgaagtct
      481 gtgctaccgt ctcgttggca gctgccactc acctgtgctg cctgcaggct aaacgtcgca
      541 ggggacaccc tagcgaccta ccaaagctgg ctaatcgctt ctacagtccg atcgaccagg
      601 acttggccac cttccgggcc gatgcggccg ctttgaatca ctgcgccgta gccacctccc
      661 tgaacagccc gagccgtggg caaatatcct cccgatccga cggccaactg gacgacaaat
      721 gtctgcacgg ccggactacg ggccttgatc tgaattccgt aatcggtgta aagctcaaac
      781 gggttcgtgg caatggcgat atcgccaagg cgaatcacat gcaattccat ggggagcgac
      841 ccaatctccc ctgactgctg cttttcgtaa agatccacaa ccctttgatg ccacaagtag
      901 ttccagcgct cagacggttt gtccgcgtac tccgcggcca atttgcgtgc ttcttctgcc
      961 tcggcctctg tcacccgacg gtaaggaagc tcaattgttt gcaccacatg ctttaggacg
     1021 acgtcctttc gcatatccag tttggctacc tcataggcct cctcccaacc acggacgatt
     1081 cgccgcgcga tctcgtccaa tcgcgacaga tttcgaagct ttcgcatcct ctcgtcggcg
     1141 gctttgccat acataagatg cggcgtgaca tccccccctg ccccggtcaa tcccaggatg
     1201 gccaggtctg acccataacg gctgcgaagg agctcacgta cgggatgcca aaaatcagca
     1261 tgaatcacgg acaggccttc cacttcctgg gcgggacagg caatattgat ggccgtggcg
     1321 atgagctttc cctcattatt ccagaaaaag aggacctcaa gattgtggtc ttcctggctt
     1381 tccatccctc ggaactcggg gacattggtg gcgccataca tttttgcgga gccatcggca
     1441 taaacggcac gtcggttttg ggcgacgaca gcctggcctt gtccccatcc cacttgcgca
     1501 ggttggcgtt tttcccatgc ctgcccgata gcttgggcaa tctgggtcgt catccaatcg
     1561 gtatatgcag aaggacgcat caccccagtc tctggcaggg tgtatttacc ctccacagta
     1621 accggggcgg tatgtgtatg cgtggcacaa aggaatatct tgaggggatc gagatcattc
     1681 ttaagccgaa ccaccttctc ccgca
//
LOCUS       DAOPTR010000937         1699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_55370_length_1699_cov_2.833942, whole genome shotgun sequence.
ACCESSION   DAOPTR010000937 DAOPTR010000000
VERSION     DAOPTR010000937.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55370_length_1699_cov_2.833942"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PKI05_14670"
     CDS             complement(<1..258)
                     /locus_tag="PKI05_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HOA53494.1"
                     /translation="MSLFSSIRMASNTMRADQIALQVIGQNIANAETPGYIREEIALK
                     PAPIQKLGGLPLGLGVQVAAVIQKLDQFLEERLRASVSDRAG"
     gene            complement(303..791)
                     /gene="flgN"
                     /locus_tag="PKI05_14675"
     CDS             complement(303..791)
                     /gene="flgN"
                     /locus_tag="PKI05_14675"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HOA53495.1"
                     /translation="MHEEWERELAKFLQEVTTIQGRLIDLLQRKQRLLAGADVEGLAA
                     LAPEEGTIEEQLKQCLARRAELLEKAAGQGLPADRITNLAGAVGASRQLKRQLAEAAH
                     QARLVQIHNLTNWMLNQRALIHLSQLIEIIATGGRRHPTYHYDSNQSLPETQGVLVDH
                     NA"
     gene            complement(877..1374)
                     /locus_tag="PKI05_14680"
     CDS             complement(877..1374)
                     /locus_tag="PKI05_14680"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HOA53496.1"
                     /translation="MTMLRSEAVSQTHPLAVIRSPSNGRSRENTSQPFVAYLDAVANR
                     TAAPSPFWTGNAASAQFESRDVTTPPQDTQQDKQGHGISPTNSEDEELEKVFRRFVGQ
                     TFFGLMLKEMRKSIHKTPYFHGGLAEQIFEQHLDMTLAEKLADASGDKFSKPMYELFQ
                     LQRRG"
     gene            complement(1408..>1699)
                     /locus_tag="PKI05_14685"
     CDS             complement(1408..>1699)
                     /locus_tag="PKI05_14685"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOA53497.1"
                     /translation="LMDVAVGPRVVVNERTGSIIISGDVEIGPVVVTHGNMVIQAGGA
                     QFVSVDSAQTQTTKLKALVDALNALNTPPQNIIQIIKGLEKNGKLHGRLIIE"
BASE COUNT          334 a          483 c          460 g          422 t
ORIGIN      
        1 gcctgcccgg tcgctcaccg acgcccgcag ccgttcttcc aagaactgat caagcttctg
       61 gatgacggcg gcgacctgaa caccgagccc gaggggaagc cccccgagct tttgaatggg
      121 cgccggcttg agggcgatct cctcgcggat gtatccgggc gtttcggcat tggcgatgtt
      181 ctggccgatc acctggagcg caatctgatc ggccctcatc gtattgctcg ccatgcggat
      241 ggaactgaac agcgacatcg ctttatcctg tatccgtcat ttgcctaaaa gccgcagttt
      301 ttctaggcat tgtgatcaac caaaacaccc tgtgtttctg ggagagactg attgctgtca
      361 taatgatatg tcggatggcg gcggcctccg gtcgcgatta tttcaattaa ctgcgacaag
      421 tgaatgagcg cccgttggtt gagcatccaa ttggtaagat tgtgaatttg aactaaacgc
      481 gcttgatggg ctgcctcggc aagttgacgc ttgagttgcc ggctagcacc aacggccccg
      541 gccaagttgg tgattcgatc cgcaggtaac ccctggccgg cggccttctc gaggagctcc
      601 gcccggcgtg ccaagcattg tttcagttgt tcttcgatag tcccttcttc gggggcaagg
      661 gccgccagac cctcgacatc ggctccggct agcaatctct gttttcgttg caagaggtca
      721 atgagccgcc cctggatggt cgtcacctcc tgcaagaatt tcgccaactc tcgttcccac
      781 tcttcgtgca ttgcgatcaa cctcagacac tacccgtacg tgattgtgag tcagatgttt
      841 tccatgagtt gcacgtcacc ccgctggtcg gtcgtcctag ccacgccgtt ggagctggaa
      901 cagctcatac atcggtttcg agaatttgtc gcccgacgcg tcggccagtt tttcagccaa
      961 cgtcatgtcc aagtgctgtt cgaagatctg ctctgccagg ccaccatgga aatagggtgt
     1021 tttgtggatc gacttccgca tctccttgag cataaggcca aagaacgttt gccccacaaa
     1081 ccttcggaag accttctcca gttcctcgtc ttctgagttt gttggggaaa tcccatggcc
     1141 ttgcttatct tgctgcgtgt cttggggggg cgtagttacg tcacgacttt caaattgcgc
     1201 agaagccgcg ttgccggtcc aaaacggtga cggggctgcc gtacggtttg cgaccgcatc
     1261 gagataggcc acaaacggtt gagaagtgtt ctcccttgac cgcccgtttg aaggcgaacg
     1321 aatcaccgcc aaagggtggg tttgtgaaac ggcctcgctt cgcaacatcg tcatgaatta
     1381 accctactct tttgctcaag acatgattca ctcgataatc aatcggccgt gcagtttgcc
     1441 gttcttttct agacccttga tgatctggat gatgttctgg ggtggtgtgt tcaaggcgtt
     1501 gagggcatcg acaagggcct tgagtttggt cgtttgcgtc tgggccgagt ccacagaaac
     1561 aaactgggca cccccggcct gaatcaccat gtttccgtgg gtcacaacca cgggaccaat
     1621 ttccacatcg ccgctgataa tgatgctgcc cgtccgttcg ttgaccacga ccctcggacc
     1681 gacggccaca tccatgagg
//
LOCUS       DAOPTR010000938         1697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_55457_length_1697_cov_3.699147, whole genome shotgun sequence.
ACCESSION   DAOPTR010000938 DAOPTR010000000
VERSION     DAOPTR010000938.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55457_length_1697_cov_3.699147"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PKI05_14690"
     CDS             complement(<1..393)
                     /locus_tag="PKI05_14690"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: protein motif:HMM:NF017174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HOA53498.1"
                     /translation="MSTQTLFRKGAMVALLATGFVLGFLANYLWVTGHAAEQTGQTAA
                     TGEVKRVMRVGQVIGLRPEKKDYYIQLHANTWPGVLKRIRDSNIRNYSIYLAELEGKL
                     YLFSYFEYVGDDFEADMAKIAADPETQRW"
     gene            complement(568..>1697)
                     /locus_tag="PKI05_14695"
     CDS             complement(568..>1697)
                     /locus_tag="PKI05_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOA53499.1"
                     /translation="PNIDIWEDTFTIDLLTWEGRCRGAMVWNHRHGRTLIWAKQTILA
                     TGGCGQLYRETTNPEVATGDGMALAYRAGAELRDMEFIQFHPTVLYIAGSARHLITEA
                     MRGEGAWLVDRHGHRFMFDYDPRGELAPRDIVSSAIVEQMEKTRHPCVYLDLTHLDPN
                     KIRQRFPGITKICEEFRLDLTRDRIPVRPGAHYMMGGVTVDLVGRTTVPGLWAAGEVT
                     SSGLHGANRLASNSLLEAIIYGRRAGEGASEAAKQEPDDFSVLPLANAPAPSPHEPLD
                     LVDIRNSVKSLMWRSMGVRREKEGMMEALEDVERWCSYVLPRQFNHPQGWELQNMLTI
                     ARLIIQAALTREESRGCHFRTDFPFPREEWQQRHISFRYQP"
BASE COUNT          379 a          476 c          454 g          388 t
ORIGIN      
        1 ccagcgttgg gtttccggat cagcggcgat cttggccatg tcggcttcaa agtcgtcacc
       61 aacgtactcg aaatagctga ataggtaaag tttgccttct agttcagcca gataaatcga
      121 atagttgcgg atgttcgagt cgcggattcg tttaagaacg cctggccaag tgttggcatg
      181 gagctggata tagtaatctt tcttctcggg acgtaggccg atgacctggc cgacacgcat
      241 aactctcttg acttcgccgg tagcggctgt ctgtcccgtt tgctctgccg cgtgacccgt
      301 cacccagaga taattggcga gaaatcccaa cacgaagccc gtggcaagca acgccaccat
      361 ggcacctttg cggaacaaag tttgggtact cattcgatcc tccgttgttc aaaaaccagg
      421 gtcagcgaaa acactctttt ggtgaccaac cagcttaatt tgggattatt cacacacagc
      481 cagcattgtc aagcgactgt ttctaacggt gctgctgccg ttgaagcgac gagcgtggcc
      541 caaacgataa ccttccctag ccctgtttta cggttgatat ctaaacgaga tatgacgttg
      601 ttgccactct tcacgcggaa atgggaaatc tgtgcggaaa tggcagccgc gagattcttc
      661 tcgggttaaa gcggcttgaa tgatcagtct ggcgatagtg agcatatttt gcaattccca
      721 gccctggggg tgattaaact gacggggcaa gacatagctg caccaacgtt ccacgtcctc
      781 taaggcttcc atcatgccct ctttttcccg tcggacgccc atactgcgcc acatcaagct
      841 cttcaccgag ttgcgaatgt caacgaggtc aaggggctcg tggggcgaag gcgcaggggc
      901 attagccagc ggcaacaccg agaaatcgtc aggctcttgt ttggcggctt cgcttgcccc
      961 ttcgcctgcc cgacgcccat agataatggc ttcaaggaga ctattcgaag ccaaacggtt
     1021 agcaccgtgc aaaccagaag aggtgacctc acccgccgcc cacaagccag gcaccgttgt
     1081 tcgaccgacg agatcgaccg taaccccccc catcatgtaa tgggcgccgg gtcggacagg
     1141 aatccgatcg cgggtcaaat cgaggcgaaa ctcctcgcaa atcttggtga tgccgggaaa
     1201 tcgttgccga atcttgttcg ggtccaaatg cgtgagatca agatagacgc agggatgccg
     1261 cgttttttcc atttgctcca cgatggcgct gctcacgatg tcgcgggggg caagttcacc
     1321 gcggggatcg taatcgaaca tgaagcgatg tccgtgtcga tcaacgagcc aggcaccttc
     1381 acctcgcata gcttccgtaa ttaggtgccg cgcacttccg gctatataga ggacggtggg
     1441 gtggaactgg ataaactcca tatcccgtaa ctcggccccc gcccggtagg caagggccat
     1501 cccatcgcca gttgcgactt cgggattggt ggtttctcgg tacaattgtc cgcacccacc
     1561 ggttgcgagg atcgtctgct tggcccaaat cagtgtccgc ccgtggcggt gattccacac
     1621 catggcccct cgacatcggc cttcccacgt caacaggtca atggtgaatg tatcctccca
     1681 aatgtcgata tttggtt
//
LOCUS       DAOPTR010000939         1696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_55498_length_1696_cov_4.017063, whole genome shotgun sequence.
ACCESSION   DAOPTR010000939 DAOPTR010000000
VERSION     DAOPTR010000939.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55498_length_1696_cov_4.017063"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PKI05_14700"
     CDS             <1..978
                     /locus_tag="PKI05_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53500.1"
                     /translation="KVNGQTVSIPSGVSRVVFDAKGGQDKITITGTSGADTATFSPTQ
                     TTFEFGSSQTLVVLNGEQVKIDGKGGTDALRVYDSPRTDNYTATPTRVTGSDNLSSYS
                     LEATNFENVVLTSRAGGEDVAMLYDSPGDDTFTATPTYGEMVGTGYKLRGEGFAFLHG
                     YASAGGVDTAMLYDSPGDDEYVCTPDYNILRGENFYIRAKLFDYVHAYATAGGYDKAT
                     IKGSAGDDKIVLEPNVVRLNSRSYVHRGKFFEEITVRGGGGRDIAYVYDGVVLPGTVA
                     RGTPPVPLDASQIVYLRQLYKIQTYNEGGTGTSRTIRPAVDAVFSAYWP"
     gene            1461..>1696
                     /locus_tag="PKI05_14705"
     CDS             1461..>1696
                     /locus_tag="PKI05_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53501.1"
                     /translation="MYCQRKVMRTIVGVMLAVLATPALAQVKDMQCFAPAQVESFGGG
                     RRANEGFFISGEYLRWSISKPDVTTVGYPGLTREV"
BASE COUNT          378 a          436 c          490 g          392 t
ORIGIN      
        1 aaagtgaatg gtcaaaccgt ttctattccg tcaggtgttt cgcgggtcgt atttgatgcc
       61 aagggtgggc aggacaaaat taccatcaca ggcaccagtg gtgccgacac tgcgacgttt
      121 tcacccaccc agactacctt cgagttcggc tcttcccaga cgctggtggt gctcaatggg
      181 gaacaggtga agattgacgg caaaggggga acggatgccc tgcgggttta cgactcgcca
      241 cgcacggaca attacaccgc tacgccgacc cgcgtcacgg ggagcgataa tctgagttcg
      301 tactctttgg aggcgacgaa tttcgaaaac gtggtgctta cgtcacgtgc tgggggtgag
      361 gacgtagcca tgctctacga ctcacccggc gacgatacct ttacggcgac acccacgtac
      421 ggcgaaatgg ttggcacagg ctacaagctg cggggcgaag gtttcgcctt cctccacggc
      481 tatgcctcgg cgggcggagt tgataccgcc atgctttacg attcccctgg cgacgacgag
      541 tacgtctgca ccccggatta caacatcctg agaggtgaga acttctacat ccgggccaag
      601 ttgttcgact atgtccatgc gtacgccacg gccggtggtt atgacaaggc cacgattaag
      661 ggctcggccg gcgacgacaa gatcgtcctt gagccgaatg tcgtacggct caattcgaga
      721 agctacgtgc atcgtggcaa gttcttcgag gagattactg ttcgtggcgg cggaggacgc
      781 gatatagcct acgtctatga cggagtggtc ttgccgggaa ctgtagcccg cggaacacca
      841 cctgtaccac ttgatgcttc ccaaattgtt tatttgcgac agctttacaa gattcagacc
      901 tacaacgagg gtgggacagg caccagccga acaattcggc cagccgtgga tgccgtgttc
      961 tcggcatact ggccgtaata ggtatcggct attgggaacc cccccaatag cggcctaaag
     1021 tggggcctgg cctcaggccc ctcaaaaggg ttgtcaaaaa cagaagccca ccatgatggt
     1081 gggcttcttt tcttttttgg tttgtcgatc gcgaagagac caaattgttc gacccgacgc
     1141 cgcgatttcg tttgcgagtt tgatcagttt agcgaagacg cggcactggg ggaatgtggc
     1201 ggattgtaac ggtttgggca aacagggctg ttcgaattga tgaaatgggt tttttcgaag
     1261 tgaccattga aaggcgacga atcaaggaaa tgctgatgtc gtaggagtgt ttggtatttc
     1321 cgttacgttt tgccattctt tctcaactct taagggcgat ttttccgata caatcctcgc
     1381 aaccccatgt tgccggttct ccacgaggag tgattgtggc gaggacggaa catttgcaaa
     1441 cattgaggtg gaccaggact atgtactgcc aacgcaaagt gatgcggaca atcgtcgggg
     1501 ttatgctcgc agttcttgca accccagcct tggcccaggt caaagacatg cagtgttttg
     1561 ccccggctca agtcgagtcc ttcggaggag gccggcgggc caacgagggc ttcttcatca
     1621 gcggtgaata ccttcgttgg agcatctcta agccagatgt aacgaccgtc ggttatcccg
     1681 gcctaactcg agaagt
//
LOCUS       DAOPTR010000940         1694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_55591_length_1694_cov_2.755339, whole genome shotgun sequence.
ACCESSION   DAOPTR010000940 DAOPTR010000000
VERSION     DAOPTR010000940.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55591_length_1694_cov_2.755339"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PKI05_14710"
     CDS             <1..385
                     /locus_tag="PKI05_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOA53502.1"
                     /translation="PEDYKKRFFDKIPSAMVWRAKPILTGMTQFRQHNLMEPLRERPF
                     DLVFLKNVLIYFDNASKQVVVKNVLNVLRPNGYLVVGPAEGVGDLLRNMRRSQPWLYQ
                     KEPEQVTPAATSPAGSLLGATEPRR"
     gene            complement(431..1093)
                     /locus_tag="PKI05_14715"
     CDS             complement(431..1093)
                     /locus_tag="PKI05_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53503.1"
                     /translation="MLVVTCPSCQARLKVSPQFVGRAGKCPRCGEIVTIPQAEPPQPV
                     TSEQANQASSGSDEDLPGIEHADRLVRTFHYLVCDRLGLVATWEANGQGWMIRSGTTM
                     VRAIKNADKLPAQGDFKLVELQIVPRTDGSHRLEGLHVYQLAPRYALTRIERGEDAIL
                     GAIRGPGSLSRDQKAAVFRYIREKFMREIWEDNQALVDYLTNQDYHSPGISPPTHPTF
                     PA"
BASE COUNT          388 a          445 c          474 g          387 t
ORIGIN      
        1 ccccgaggac tataagaaac gatttttcga caagattccc agcgccatgg tgtggcgggc
       61 taagcccatt ctgaccggca tgacgcagtt ccgccagcac aatctgatgg agcctttgcg
      121 agagcgccca ttcgacctcg tcttcttgaa gaacgtgctc atttactttg acaacgcatc
      181 caagcaggtt gttgtcaaga atgttttgaa cgtactccgc cccaacggtt acctcgtcgt
      241 tggacctgcg gagggagtgg gcgatctgct acgtaatatg aggcgatcgc agccgtggct
      301 ttaccaaaag gaaccagaac aggtaacccc cgcggcaacc tcacccgcag gtagtctctt
      361 gggagccacg gagccccgtc gttaacaaga cagcacaccc cgcgcgaccg atcgagaaca
      421 catttgacct tcatgcggga aaggtgggat gcgtcggcgg cgaaatcccg ggggaatggt
      481 aatcttgatt ggtcaaataa tccaccaggg cttgattgtc ctcccaaatt tcccgcataa
      541 acttttcccg gatatatcga aacactgccg ccttttgatc acggctaagg ctcccgggac
      601 cacgaatcgc acccaggatg gcgtcctctc cccgttcgat gcgggtcagg gcgtagcgcg
      661 gggccaactg ataaacgtgg agtccctcca gccggtggga accatccgta cggggcacga
      721 tttgcaattc caccagcttg aaatcgcctt gggcagggag cttatcggcg ttttttatgg
      781 ctcggaccat ggttgttccg gaacggatca tccaaccttg cccgttagct tcccaggtgg
      841 caaccaagcc gagacggtca cacaccaggt agtgaaacgt tcgcacaagt ctatcagcat
      901 gttcgatgcc gggaagatct tcgtctgatc ctgaagacgc ttgattagct tgctccgatg
      961 tgacaggttg ggggggctcc gcttgaggta tggtgacgat ttctccgcac cgtgggcatt
     1021 ttcccgcgcg acccacgaat tgcggactaa ccttcaatcg agcttgacac gaggggcaag
     1081 tcacgacgag catctgttat cctcccgttg gagtgattcc ggcagcgcca ttctgctttc
     1141 atggtccgtc ggggttggcg aaatttcaac catggctcac ctaagatacg tatacaaccc
     1201 cactacgttg gggtgtattc cccgcctttg cgtccccaat cgcaaaagcg taacatgaga
     1261 ggataggcaa agaggaatag actcaagcgg ttggggaaga atacccgtag aaaatgggtg
     1321 agtcttccga acggattgtt ccaggggata cattgcgctc agagtctaaa cagggggtgg
     1381 ttgatggggg cagccggagg gggtaacgcg tggtgatccg tgtaacatga tccttgggcg
     1441 ctagcaggga taatcgcccg gttgtgtctt gaggatcttg gcttcatgga ttgtcatcat
     1501 gcaaggaatc cattgctaag tgcgtcgaag aacctagtgg tttgctgaga gttaatcgcc
     1561 gatatcgagt gcgttaacaa gattgcgggc ctcccgttag aatgtccttc accacgttcg
     1621 tacccagaac gcaccgttag attaatcatc acttggcgtc agtcgatagg tagctgaggt
     1681 tcatgaataa gatg
//
LOCUS       DAOPTR010000941         1693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_55619_length_1693_cov_3.753358, whole genome shotgun sequence.
ACCESSION   DAOPTR010000941 DAOPTR010000000
VERSION     DAOPTR010000941.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55619_length_1693_cov_3.753358"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..1264
                     /locus_tag="PKI05_14720"
     CDS             161..1264
                     /locus_tag="PKI05_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HOA53504.1"
                     /translation="MTPQPAIGVLLHAVGGFAAGSFYSPLKLIRRWSWESLWLVMGLG
                     AWLIAPWVAAYWTIPNLFEVLRASPMAVLFWAAFFGALWGLGNLTFGLAVRYLGMGLG
                     YAVALGFCMVFGTLIPPVYAGVFGSVADREKLVNLFVTTPGQVVLLGIAVCLGGIALC
                     GWAGLRREQETGKDLDSKETSRFALGKGFAVATVSGILSACFAFGLAAGKPIAQTAHN
                     LGAAEVFSNNASLVVILVGGLISNAVWCLMLNVKNRTFGDYVCGRFSAQLFNYGLALL
                     GGIVWYLQFFFYGMGTTKLGPQYDFSSWSIHMAFIIVFSNLWGIVFKEWHHARFITKI
                     LVFSAIVVLIASTGIIGYGNYLDLLLRKSAVST"
     gene            1445..>1693
                     /locus_tag="PKI05_14725"
     CDS             1445..>1693
                     /locus_tag="PKI05_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53505.1"
                     /translation="MSRIAALTSLLCIAFCITGSRNVFALEETGLQPYSVTWEGLEGS
                     LIDLSFLLEKPAGKQGFITIRDGHFAKPDGQRIRFWGVN"
BASE COUNT          335 a          402 c          506 g          450 t
ORIGIN      
        1 acgacgctgc caacaatctc ctccactacg agtaccgaaa gccctggagc ctcgactgag
       61 agatcttcag gtttcgggaa aaacctcgcg tgtcggcgat cgtcgaaacc aggcttggat
      121 gtaatcctaa ccggtgatcc cagggaaaaa ggctttctgc atgactcccc aacccgcgat
      181 tggtgtttta cttcacgccg tgggcggttt tgcagcaggc agtttttact cgcctctcaa
      241 attgatccga cggtggtctt gggaaagcct ttggctggtg atgggattgg gggcctggtt
      301 gattgcccct tgggtcgcgg cgtactggac cattcccaac ctctttgagg tcctccgggc
      361 cagccccatg gcggttctct tttgggcggc atttttcggg gccctttggg ggctgggcaa
      421 tcttactttt gggctcgccg ttcgatacct cggcatgggg ctgggatacg ccgtggccct
      481 tgggttttgt atggtttttg gaacactcat cccgcccgta tatgcgggag tatttggttc
      541 cgtggccgac cgcgaaaaac ttgttaacct ttttgtgacg acaccgggtc aggtcgtcct
      601 cttgggcatc gccgtgtgtc ttgggggaat tgccctgtgt ggctgggcag ggttacgacg
      661 agagcaggaa accggaaaag accttgattc caaagaaaca tcgcggtttg cccttggcaa
      721 gggattcgcg gtggccacgg tctcagggat tctcagtgcc tgcttcgcct ttggcctggc
      781 tgcggggaag cctatcgccc aaactgccca caatctcggg gctgccgaag tcttctcgaa
      841 caacgccagt cttgtggtca tcctcgtagg tggactgatc agcaatgcgg tgtggtgttt
      901 gatgctgaat gtgaaaaacc gaacatttgg cgattacgtt tgtggtcggt tttcggccca
      961 gttgttcaat tacggactgg ccctcctggg aggaattgtc tggtaccttc aattcttctt
     1021 ttacggaatg ggaacgacca agctcgggcc gcaatacgac ttctccagtt ggtcgattca
     1081 catggctttt atcatcgtct tttccaatct gtgggggatt gtgtttaagg aatggcacca
     1141 tgcccgtttc ataacgaaga ttctcgtgtt ttccgcaata gtggtgttga tcgcttcaac
     1201 aggtatcatt ggatatggga attacctcga tctgctgctg aggaagagcg cggtttccac
     1261 gtaaagacct tggggttcat ccggccaatc gcgaggcctg ggggaagttt ctgccaaatg
     1321 tggttgagca gggggtaagg aagaaccaag acaaatgggg gcacacttgt acgagatcaa
     1381 acggtagcgg tgttccggat ggcttgagcg aaagtcttga gacaatgttg tgggaggcta
     1441 cgtcatgagc cgaatagctg ctttgacgtc tctgttgtgc attgcgtttt gcatcacagg
     1501 gtcgagaaat gtttttgcat tggaggagac agggcttcaa ccctattctg tgacgtggga
     1561 aggattggag ggaagcctga tcgacctgtc atttcttctg gaaaagccgg cggggaagca
     1621 ggggtttatc acaattcgag atggtcactt cgcgaagcct gatggccaac gcattcgctt
     1681 ttggggagtc aat
//
LOCUS       DAOPTR010000942         1692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_55675_length_1692_cov_2.649969, whole genome shotgun sequence.
ACCESSION   DAOPTR010000942 DAOPTR010000000
VERSION     DAOPTR010000942.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55675_length_1692_cov_2.649969"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PKI05_14730"
     CDS             complement(<1..707)
                     /locus_tag="PKI05_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOA53506.1"
                     /translation="MELTLTFYPIAESYFLVAGLILTLLLLWRLFPLSQQLSGGRRRI
                     LLALRVATIIVVLFVLLRPTLVYLTRTKQAASVVLLIDQSQSMSVPDEVGGKTRFDAV
                     VETLQKNAAVLQKLAEDVQIHSYAFDGQLHALPLEKGLPKLPDAPRGNQTAIGAVLED
                     VIREVAGQRVIGIIMMSDGAQRAIYPRDTPPQPAAQRLRSLDQPLYTIRFGRARAGAE
                     GRDIVLEDLFAPDRVFV"
     gene            complement(812..>1692)
                     /locus_tag="PKI05_14735"
     CDS             complement(812..>1692)
                     /locus_tag="PKI05_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53507.1"
                     /translation="PGHVLVQSRAPSGDVYVRPTSYDHPVLKPFKALAGSVPWDLFPV
                     YRYWVIDPLAENALVVLPYSDGRPCMIERVVGKGRVITVTTPVSDLPDSFPWNLLPMG
                     QAWPFLILSNQLAFYLAGTGEIRTNYLAGETVRVVIDSGGKLTGALLDLPPDALNATR
                     SEPAFPIRLSFQPDKRDILITATERVGHYRLRGGGKDEEFVFGFSVNYPGALMELDRV
                     SKGELAELFKNVSFQIAHDRSDLSRQVTAGRTGFDATPYVLMAMVLILGLEQVVANRF
                     YRAAGQEPTKQRAAAT"
BASE COUNT          399 a          499 c          442 g          352 t
ORIGIN      
        1 ttcacaaaga cacgatccgg ggcgaacaga tcttccagga cgatatcgcg gccttcggcc
       61 cccgcgcgcg cccgtccaaa gcggattgtg tagagcggct gatcaagcga ccgcaatcgc
      121 tgtgcggcgg gctgcggtgg tgtgtcccgc ggatagatcg ctcgctgcgc cccatcactc
      181 atcatgatga ttccaataac ccgctgcccg gctacttcgc gtatcacatc ttccagcaca
      241 gccccgatgg ccgtttgatt gccacgcggc gcgtccggca acttagggag gcctttctcc
      301 aacggaaggg catgtaactg cccatcgaat gcatagctgt ggatttgaac atcctccgca
      361 agtttttgga gaacggcggc gttcttctgg agcgtctcca cgacggcatc aaaccgcgtc
      421 ttccccccca cctcatccgg aaccgacatg ctctgcgatt ggtcaatgag aagaacgaca
      481 ctggcggctt gctttgtcct tgtcagatag accaaggtcg gccgaagcag tacaaataag
      541 accacgataa tggtggcaac acgcagcgca aggagtatcc tgcgccgtcc ccctgacagt
      601 tgctgactga gtggaaaaag cctccacagt agcaacagcg tcaaaatgag gccggctacc
      661 aggaaataac tttcggcaat tggataaaac gttagtgtta gttccacacc gataatcaat
      721 aacgccgttc tgtggccgtt cccaaataaa gtactaccct gttcatgccc ccacacactt
      781 gctgttcacg cacgttcccc tttgaaccac ctcatgttgc cgctgccctc tgtttggtcg
      841 gctcttgccc cgcagcgcga taaaaccggt tcgccacgac ctgttccagt cccaaaatga
      901 gaaccatcgc catcaggaca tagggtgttg catcaaagcc tgttcgccca gcggtcacct
      961 gtcggctcaa gtccgagcga tcatgggcta tctggaacga aacgttctta aagagttcgg
     1021 cgagctctcc tttggagacg cggtcgagtt ccatcaatgc cccggggtaa ttgacgctaa
     1081 acccgaagac aaattcctca tctttgcccc ctccgcgaag acgatagtgg ccgacgcgtt
     1141 cggtagctgt gattaagata tctcttttgt ctggttgaaa actcaagcga atgggaaacg
     1201 ctggttctga ccgcgttgcg ttgagtgcgt ccggcggaag atcaagaagc gccccggtca
     1261 gttttccgcc gctatcgatg accacacgaa cggtttcacc agccaaataa ttagttcgta
     1321 tttcaccggt tccggccaaa tagaaggcca gttggttact aagaatgaga aacggccaag
     1381 cttgacccat cggcaggaga ttccacggaa atgaatcagg caggtccgag accggcgtgg
     1441 tcaccgtgat gacccggccc ttgcccacga cacgttcgat catgcatggc cttccatcgc
     1501 tgtaaggcag gacaaccaag gcattctctg caaggggatc gatcacccaa taacgataaa
     1561 ccggaaaaag gtcccacggg accgaaccgg ccagggcctt gaacggcttc agcacggggt
     1621 ggtcataact tgtgggccga acatagacat ccccacttgg tgcgcgagac tgaacgagaa
     1681 cgtggcctgg ca
//
LOCUS       DAOPTR010000943         1691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_55705_length_1691_cov_3.385086, whole genome shotgun sequence.
ACCESSION   DAOPTR010000943 DAOPTR010000000
VERSION     DAOPTR010000943.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55705_length_1691_cov_3.385086"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PKI05_14740"
     CDS             <1..634
                     /locus_tag="PKI05_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53508.1"
                     /translation="AFMTFVLTFSSIGLPGLNGFVGEFLVLLAMFQRAWSHGGGELAL
                     AILATSGVVLGAWYMLTLVGRLFFGPLNEPLASPVAERDGQAFATPGGDEDWKTYHVS
                     TGQHAIEDLNLREILALAPLFVFIVWIGIQPNYFLRRMTPAVRQIERSITLAVSPSDR
                     DGQEVLGHIVMPQPSSPRAKLHLAEQDNPTARLALTENKDVCGVSSVNHD"
     gene            600..>1691
                     /locus_tag="PKI05_14745"
     CDS             600..>1691
                     /locus_tag="PKI05_14745"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOA53509.1"
                     /translation="MYAVFHLLITTDWLPEVFLGLAGVLCLVLSPFGLRRGWIYVLAG
                     LGILAALMAVVLHTDYLARTPDLFQAFARELILFIGFLLLLSSGGAPVSYDGERVGTL
                     ILAVLGAMFAVRAQDLIVLFVGLELLSLATYVLLYLDREGPHRREGATKYFYLSILAS
                     AIFLFGVSYLYGLGRSVSYKDLMIAGTEHGFSGPLASIALVFILAGLAFKIAAVPFHF
                     YAPDVYQATSHRNAALLSALPKIAGIIVMMRIILGFPLTAAPQSWQIVLALSAITMTV
                     GNVLALWQNHIRRLLAYSSVAHAGYMLIGLTVAMTYAQPGSHPWDGRTAVLLYLLVYV
                     VATVGAFAALGLLGVQGRPVETLEDLRGLA"
BASE COUNT          275 a          472 c          480 g          464 t
ORIGIN      
        1 cgctttcatg acattcgttt tgacattttc cagtattggg ctaccaggcc tcaacggttt
       61 cgtgggagaa ttcctcgtgc ttctcgccat gttccaaagg gcgtggtccc acggcggtgg
      121 agagttggcc cttgccatat tggccacgtc gggagttgtc ctaggagcgt ggtatatgct
      181 gactcttgtt ggtcggctgt tctttggccc tctcaacgaa cctttggcat cgcctgtggc
      241 ggagcgcgat ggccaggcct tcgccactcc cggtggcgat gaagactgga aaacctacca
      301 cgtttcaacg gggcagcacg cgatcgagga tctaaacctt cgtgagattc tggccttggc
      361 ccccttgttc gtcttcatcg tgtggattgg cattcagccc aattactttt tacggcgaat
      421 gacgccggcc gtgcggcaga tcgagcggtc catcactctc gccgtctcac cgagtgaccg
      481 ggacggccag gaggtcctgg ggcacatcgt gatgccgcaa ccttcgtcac ctcgcgcgaa
      541 gctgcacttg gccgagcaag ataatcctac agctcgattg gccttaacgg aaaacaaaga
      601 tgtatgcggt gtttcatctg ttaatcacga ctgactggct tcccgaggtt tttctcggct
      661 tagctggtgt cctttgtctg gtcctgtccc cttttggact gcgccgaggt tggatttatg
      721 tcctagctgg actgggcatt ctggcggcct tgatggccgt cgtccttcac acagactatt
      781 tggcgcgaac gcccgatctg ttccaggctt ttgcccgcga actcattctg ttcatagggt
      841 tcctcttgct cctttcaagt ggtggtgccc ccgtgagcta tgatggggag cgagtcggca
      901 cgctcatctt ggccgtcctg ggggccatgt tcgctgttcg cgctcaagac ctcattgtgc
      961 tttttgtggg tctcgaattg ctctcactgg caacctatgt gttgctttac ctggatcgtg
     1021 aaggaccgca ccggcgtgaa ggtgccacca agtatttcta tttgagtatt ttggcctcgg
     1081 cgattttctt gttcggtgtc agttacctct atggactagg gagatccgtt agttacaagg
     1141 acctgatgat tgccggaacc gagcacgggt tttccggacc gctggccagt attgccctgg
     1201 tgtttatttt ggcgggacta gcgttcaaga ttgccgccgt gccctttcat ttttatgctc
     1261 ccgacgtgta tcaggcgaca tcccaccgta acgcggcatt gctttcggcc ctgccgaaga
     1321 ttgccgggat catcgtgatg atgcgcatca ttctggggtt tccgctgacg gccgcacctc
     1381 aaagctggca gatcgtcctg gccctttcgg caatcacgat gacggtgggt aacgttcttg
     1441 ccctttggca gaaccacatc cgacggctac tcgcatattc ttccgttgcc catgccggat
     1501 atatgttgat cggtttgacg gtggccatga cctacgccca acccgggtct catccgtggg
     1561 atggcagaac ggccgttctg ttgtacctgc ttgtgtatgt cgtggccaca gtgggggcct
     1621 ttgccgctct cggccttctt ggtgttcagg gacggcccgt tgaaaccctt gaggaccttc
     1681 gcggcctggc g
//
LOCUS       DAOPTR010000944         1691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_55707_length_1691_cov_3.319071, whole genome shotgun sequence.
ACCESSION   DAOPTR010000944 DAOPTR010000000
VERSION     DAOPTR010000944.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55707_length_1691_cov_3.319071"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="PKI05_14750"
     CDS             <1..688
                     /locus_tag="PKI05_14750"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA53510.1"
                     /translation="QHPRHFGEMVQYMDKLIGKLVDLLDELNIRKHTLLIFLGDNGTG
                     GGIRSMMGDREVIGGKGKTTRAGMRVPLIVNGPGMVRSGVVCSDLVDSTDIFPTICEA
                     AGIPIPDGLVLDGKSFWPQLCGQPATPRKWIYCWYSPRQGADTRVTEFAFNQRYKLYR
                     DGRFYDCELDPEEKQPLEDSQLSEDTRQIKEMLKQVLNAHVGIRPAHLDEQLPPPDRP
                     ATRQRRARQQ"
     gene            832..>1691
                     /locus_tag="PKI05_14755"
     CDS             832..>1691
                     /locus_tag="PKI05_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53511.1"
                     /translation="MARYLLLGFVALSTLTVRIPAAELQFELHRIGSFRSEACGVADF
                     NGDGKLDVVAGPFVYVAPDWKAYKIREIKGQVDEAGKGYMWNFADLPLDVDGDQRVDV
                     ISCDWFEKCLDWHRNIGFGGDLWPRSVIDVSLNHETAQLVDIDGDGQKREILPEVVET
                     YWFEIVRDPAGGVRFEKHIISQKKMNFGGGVGDINGDGRPDVIRPDAWFEAPADPRSG
                     QWVEHPIALGGREEGQSEHTAQILVHDVNGDGLNDLIASSAHRYGIYWYEQVREDGTI
                     RFRRHLIDNT"
BASE COUNT          400 a          382 c          514 g          395 t
ORIGIN      
        1 tcagcatccg cgtcattttg gggagatggt ccaatacatg gacaaattga tcgggaaact
       61 tgtggatttg cttgacgagc taaacattcg taagcacacg ctgctgattt tcctgggtga
      121 caacggtaca ggcgggggta tccgctcaat gatgggcgat cgggaagtga ttggcggcaa
      181 agggaaaacc acgcgggctg gaatgcgtgt gccgttgatt gtgaatgggc cagggatggt
      241 tcggtctgga gttgtctgct ctgacctggt tgatagtacg gacatttttc cgaccatctg
      301 cgaggccgcg ggaataccta tccctgacgg tttggttctc gacggcaaga gcttttggcc
      361 acaattgtgt ggtcagcccg cgacaccgcg aaaatggatc tattgttggt attctcccag
      421 gcagggagcc gatacccgtg tcacggagtt cgcattcaat cagcggtata aactgtaccg
      481 tgatggccgt ttctatgact gtgagttgga tccagaggaa aagcaacccc ttgaggatag
      541 ccaattatcc gaggataccc ggcagatcaa ggaaatgcta aagcaggtcc tcaatgccca
      601 tgtgggtatt cggcctgccc acctcgacga gcagttgcca cctccggatc gtccggcaac
      661 acgccagcga cgggcacggc agcagtaacc taaggacttg catgtcacca ctccggaggt
      721 taagttaaac cggcataaga aagtcttggc gaagtgaagc atcgtaaagg tgtctcagtg
      781 cctcaccaat gattcaggtg aggtgtgggc gatgattaaa ggaaaaaagg aatggctcgg
      841 tatcttctgt tgggatttgt tgctctttcc acactgacgg ttcgcattcc agcggcagag
      901 ttgcaatttg aattgcatcg aattggcagt tttcgcagcg aggcctgcgg cgtggccgac
      961 tttaacggcg atggtaagtt ggacgtcgtt gcggggccat ttgtttatgt cgcacccgat
     1021 tggaaggcat acaagatccg cgagatcaaa ggccaagttg acgaagcagg gaagggctac
     1081 atgtggaact ttgcggatct tcctctggac gtagatggcg accagcgcgt ggatgtgatt
     1141 tcgtgtgatt ggttcgagaa gtgtttggat tggcacagga atatcggctt cggaggcgac
     1201 ctttggcctc gtagcgttat tgatgtgagt ctcaatcacg agaccgcaca acttgtcgat
     1261 atcgatgggg atgggcaaaa gcgcgaaatt ctaccggaag tcgttgaaac atactggttt
     1321 gaaatcgtgc gcgatccagc gggaggtgtc cggttcgaaa agcatatcat tagccaaaag
     1381 aaaatgaatt tcggcggtgg cgtgggcgac atcaatggtg atggccgacc ggatgtaatt
     1441 cggcctgatg catggtttga ggcacccgcc gatcctcgga gtggtcaatg ggtagagcat
     1501 ccaattgcct tgggcggacg ggaagagggg caatcggaac acacggccca aatcctcgtc
     1561 catgacgtga atggcgacgg cctgaacgat ttaatcgcca gttcggcaca ccggtatgga
     1621 atctactggt acgagcaggt tcgcgaggat gggacgattc gttttcgacg acatctcatc
     1681 gacaacactt g
//
LOCUS       DAOPTR010000945         1689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_55782_length_1689_cov_3.668299, whole genome shotgun sequence.
ACCESSION   DAOPTR010000945 DAOPTR010000000
VERSION     DAOPTR010000945.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55782_length_1689_cov_3.668299"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..954)
                     /locus_tag="PKI05_14760"
     CDS             complement(178..954)
                     /locus_tag="PKI05_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53512.1"
                     /translation="MLQTLGIVDACFASDKERRRAAGRLQGKSILEWVTRRATDCQQL
                     DGVIVVTSSDPANLFVKDLVPLDVPVFVGEGDDHLTIWAKALEAYPCREFVRFETACP
                     FVDQLLVDRLVKEAHRLDEPADYVGYRSHNGTPAMLTSVPVYGELVLTKTLHRLCERL
                     RNPSDRAHPTRYIYSHPERFKIHWLPIPPGLDRDDIRLVLVDEDDWEVHQIIVDTLGP
                     ECDGHHIAHLIERCPSLRKQMEALNEKKSRQAIVKARPSV"
     gene            complement(1135..>1689)
                     /locus_tag="PKI05_14765"
     CDS             complement(1135..>1689)
                     /locus_tag="PKI05_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53513.1"
                     /translation="ETVNQMAEALERCLSHASGTGRLVQARALPETAKTDGAADQVPT
                     AQPVPQLKLGDREARPSLEGDLKSGERNQSTEVIGASPKQASQSKRQGVALSKRRRVG
                     YAVGIITLVLIGGLLAFFAPWRKPQQKATPESKKPRPSIQRDTTKEAAPARRPEDDPE
                     AFRQKLEEWMRTQMAPKQSGKPGK"
BASE COUNT          371 a          496 c          434 g          388 t
ORIGIN      
        1 gcttatctca aaacgatgcc cgaccccaga ctgccagccc gctcttcaaa ttcggaagcc
       61 caggtggagc tatccaactg acagcctatc ccaaggaatc tccctctcct tccgggaaag
      121 ggttaggatg agggcaagcg aacgtcggaa tcgattgttc gcacggggga ataactttca
      181 gacggatggc ctggccttga ctatagcttg acggctcttt ttctcattta gcgcttccat
      241 ctgtttacgc agagaagggc atcgctcaat aaggtgggcg atgtgatgac cgtcacactc
      301 aggcccaagg gtatccacaa tgatttggtg aacttcccag tcgtcttcat ccaccaagac
      361 caagcgaata tcgtctcggt ctaggcccgg tgggatggga agccaatgaa tcttgaatct
      421 ttctgggtgg gaataaatgt aacgggtggg atgcgcgcgg tccgaaggat ttcgcaaccg
      481 ttcgcagagg cggtgaagtg tcttggtcag caccagctcc ccgtacacag gaacactggt
      541 tagcatcgct ggtgtgccgt tatggcttcg gtaaccgaca taatcagcag gctcatcgag
      601 acggtgggct tccttgacca ggcgatcgac cagcagttga tccacaaagg ggcacgccgt
      661 ttcaaatcgc acaaattccc ggcaaggata agcctccaag gctttggccc agattgtcaa
      721 atgatcgtcg ccttccccga cgaataccgg cacatcaagc ggaacaaggt ctttgacaaa
      781 gaggtttgct ggatctgacg aagtgacgac gataacgccg tccaactgtt ggcagtcggt
      841 tgcccggcgc gtcacccatt ccagaataga ctttccttgc aaccgcccag cagcccggcg
      901 tctttcctta tccgatgcaa aacatgcatc cacaattccg agtgtttgaa gcatccggcg
      961 caacctcctt caaaagtcct ttttgatagc caagcattcc ttgatctcac tcgccgccgg
     1021 accagcggtg tcaattctgc ttcaatatac ccggctaaca atcccgcagt caaccatcac
     1081 gaaagaaaag cgacgtacgc ccgcattcaa cacgaataac cttcgctgtt tcattcactt
     1141 accgggctta cctgattgct tgggagccat ctgcgttcgc atccattctt cgagtttctg
     1201 ccgaaatgcc tcggggtcgt cctcgggccg acgtgccggc gccgcctcct tcgtagtatc
     1261 acgctggata ctgggacgcg gcttcttgga ctcaggggta gccttctgtt gaggtttcct
     1321 ccatggagcg aagaacgcaa gcaaaccccc aatcagtacc agcgtgataa tacccacggc
     1381 gtaccccacc cggcgccgct tactcaaagc cactccttgc cgtttggact gggatgcctg
     1441 ctttggtgaa gcaccaatca cttctgttga ctgatttctt tcgcctgact tgaggtcacc
     1501 ttcaagcgat ggtctcgcct cgcggtcacc gagtttaagt tgaggcaccg gttgggctgt
     1561 cggcacttgg tccgcggcgc cgtcagtctt ggcggtctca ggcaacgcgc gagcttgaac
     1621 aagtctgccc gtccccgacg cgtgagaaag acatcgctca agcgcctcgg ccatctgatt
     1681 gaccgtttc
//
LOCUS       DAOPTR010000946         1682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_56096_length_1682_cov_3.142594, whole genome shotgun sequence.
ACCESSION   DAOPTR010000946 DAOPTR010000000
VERSION     DAOPTR010000946.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56096_length_1682_cov_3.142594"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PKI05_14770"
     CDS             <1..247
                     /locus_tag="PKI05_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53514.1"
                     /translation="QKGATGNDGLILEAQLAVDGRSGKYPQFQGNVATVYTYPLSKGG
                     ASNNHYGGNAETYMNVGEAMGKAMVELLNEAKKDPLP"
     gene            complement(704..>1682)
                     /locus_tag="PKI05_14775"
     CDS             complement(704..>1682)
                     /locus_tag="PKI05_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53515.1"
                     /translation="ALEKYPHIQFQVIIYGEDWPELARYGKGDRAAQLVARYAQARFS
                     AFPNVYWCISNDAHISSGSGARNADPAVINQIGNDMKRREPWGTLLTNHQQRFQGYSF
                     VDAAWSDIVTLEDRDQVAGAIVLQYQRLANCPVVLDEDRYGIYISPQHDRYFFRRLMW
                     ANLLSGGHATYGGLNTYEPFDGPKGIRGIHGYRTAMADGRLDDGAADFLHIKRFFEES
                     GLTLVGLEPNDAMAGNNGHLVKVMEGKKTIIAYLQNPDSPTPETANVAETPATCRMHL
                     PPGQWRIRWYDPRSGKWHPDPERREITGGYTRDFKSPFSGDAVLLLQLP"
BASE COUNT          385 a          488 c          456 g          353 t
ORIGIN      
        1 ccagaagggc gccaccggca acgatggatt gatcctcgag gcacagctcg cagtggacgg
       61 cagaagcggg aaatatcccc aatttcaggg caacgtggct accgtttaca cttacccgct
      121 ttctaaaggc ggcgcctcca ataaccacta cggcggtaat gccgagactt acatgaacgt
      181 cggagaggcg atgggcaagg cgatggtcga actcttgaat gaagcgaaaa aagatccttt
      241 accgtaaaca cgccggcccg ccctacgttc cttctgtaat cgctcacaga gaacaaagga
      301 ccttcccgaa agatacccgt aatagcaggg ccagtgcctt agccacctag ggagggtcag
      361 cgtccctggc gacccagaga ggtgacctca ggaaggtcgc cgtccgcggt gaccaaatca
      421 tcggacggcg gggacaccgt cgctgcctcc tcttcgccca ccctacaaag tccttaccgc
      481 gcaacccctt gagtcgcaag aagatgccga tatgctctgt aaacggttag gttcccccta
      541 cctcgccaaa aaccggcgga gcacaatatc gttgtggctt gaccatgtca cctctcacag
      601 ataagactga ctttccatct gctcgtcaca cccattttca tccagagtcg cgggcgtgtg
      661 ttgcaaaagc tccactgcgg gtggtcccat caagcttgtg gagtcaggga agttgtaaca
      721 aaagtactgc atcaccagaa aatggtgact tgaaatcccg ggtataaccg ccggtgattt
      781 cccgacgctc gggatctggg tgccattttc ccgagcgggg atcgtaccag cggatgcgcc
      841 actggccggg aggtaggtgc atccgacaag tggccggcgt ctcggccacg ttggcagtct
      901 ccggcgtggg agaatcgggg ttttgcagat aagcgatgat cgtcttctta ccctccatga
      961 ctttgaccaa atgaccgtta ttaccggcca tcgcatcgtt aggttctaag ccgaccagtg
     1021 ttagcccact ttcttcaaag aatcgcttga tgtgaagaaa atccgccgca ccgtcatcga
     1081 gacggccgtc cgccatggcg gtccgatagc cgtggatccc ccggattcct tttggtccgt
     1141 caaatggttc ataggtattt aatccgccgt aagtcgcgtg gcctccgctg agcagattgg
     1201 cccacatgag ccgccggaag aaataccggt cgtgttgagg tgagatgtaa atcccgtacc
     1261 gatcttcatc caacacaacg gggcagttgg ccagccgctg atattgcaac acgattgccc
     1321 ccgccacctg gtcccggtct tccaaggtga cgatgtccga ccatgcagca tccacgaacg
     1381 agtagccttg gaatcgctgc tggtgattcg tcaggagcgt tccccagggt tcacgccgtt
     1441 tcatatcatt gccgatttga ttaatgaccg ccgggtccgc gttccgggca ccagaacctg
     1501 acgaaatgtg agcgtcgttc gaaatacacc agtacacgtt gggaaaagcc gagaagcgtg
     1561 cctgggcata acgagccacc aactgggcag cgcggtcgcc cttgccgtaa cgtgctaatt
     1621 ccggccagtc ttcgccgtag ataatcacct ggaattgaat gtgggggtac ttttccaaag
     1681 cg
//
LOCUS       DAOPTR010000947         1681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_56145_length_1681_cov_3.621771, whole genome shotgun sequence.
ACCESSION   DAOPTR010000947 DAOPTR010000000
VERSION     DAOPTR010000947.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56145_length_1681_cov_3.621771"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="PKI05_14780"
     CDS             complement(<1..990)
                     /locus_tag="PKI05_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53516.1"
                     /translation="MPRERRNFLGTLSRILVTVAIARPLVTIGIGLLTAAVAIYCGIN
                     FIQIHTSRADLLNSKNEYHRRWLAYVNEFSDKEDVYVVVEGDNPNAVAPALIELAEAL
                     RREPQYFTSVLERVDKTPLKRKGLYYLSTQELQQIVSHVRQLKNTLTQLAPVLRPANV
                     CLEAAAMISQIPPGYPSHQAFGQIAQVIQYLAVVLNSPPEQLAETILTQIAANNQQGD
                     FAFGQGLGVLLDPEKTTDLREEMFFSHDGHLGLVILRFTPDRSGQFTRFAAHIRRLRE
                     ITSDVNRRYPNLRIGSTGLPILEYDEMRASSSSAMVAVLSLAGVILVFFAGFGG"
     gene            1298..>1681
                     /locus_tag="PKI05_14785"
     CDS             1298..>1681
                     /locus_tag="PKI05_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53517.1"
                     /translation="MSLARSKVTRRAFLAGSAAAVSFFGKRLPAEVEGVPIRAITKGP
                     GFHWFAYYDKFQFDPTDRYALGMKVLFEHRSPTPQDSIEIGMIDLAEGDRWIPLGETT
                     AWCWQQGCMLQWRPGSATEIVYNGRG"
BASE COUNT          405 a          413 c          486 g          377 t
ORIGIN      
        1 tccaccgaag cctgcaaaaa acacgaggat aacaccggca agcgagagaa cggcgaccat
       61 ggcggaagag ctacttgccc gcatctcgtc gtactctagg atgggtaggc ctgtggagcc
      121 aatccgaagg ttcgggtagc gccggtttac atcgcttgtg atttccctca gacgccggat
      181 atgagcggcg aaccgcgtaa attgtccgct tcggtcaggg gtgaaccgca agatcacaag
      241 acccaggtgg ccgtcatgag aaaagaacat ttcctcacgc aggtccgttg tcttttctgg
      301 atcgaggagg acgccgagtc cttgtccaaa agcgaagtcg ccttgttgat tgttcgctgc
      361 aatttgcgtg agaattgtct cggccagttg ttccgggggg ctgttcagaa caacggcgag
      421 gtattgaata acctgggcga tttgcccaaa cgcctggtgc gaaggataac caggagggat
      481 ttgcgaaatc attgcggccg cttccaggca cacattggcc ggccggagga caggggccag
      541 ttgtgtcaat gtgttcttta actggcggac gtggcttacg atctgctgga gttcctgcgt
      601 ggagaggtaa taaagcccct ttcttttgag aggtgtctta tcgacacgct ccaacactga
      661 tgtgaagtac tgcggttctc gcctaagggc ctcggcaagt tctataaggg ccggggcaac
      721 tgcatttgga ttatccccct ctacaacgac gtaaacatcc tctttatcag aaaactcgtt
      781 cacataggcc agccaacgcc ggtgatactc gttcttggaa ttgaggaggt cggcccgact
      841 ggtgtggatc tggataaaat tgattccgca atagatcgcg accgcagccg tcagaagacc
      901 gattccgatc gtgaccagag ggcgtgctat cgccacggtc actaagattc gcgaaagcgt
      961 tccaaggaaa ttacgtcgtt cgcggggcat ccgtgacccc tgaaagaatg ttagaccaca
     1021 caaagatttg tcacaaccag ggcgttatag tagcataaag gtcctttttg cgacaagagt
     1081 gataaggaag ccaactggcg caaagaatag gccagaagac attcggtcac aggagaggcc
     1141 acgttcacgg tgtcaaagca aaaaggcatg tgtttgagac gagaccctcg gcctgcctct
     1201 ttgatctggc aacggcttcg gttaggataa ccagccagaa tcgcgcgtta acacaaaggt
     1261 ttactaactt tctcgattta aatgagggga attacctatg tcccttgcaa gaagtaaggt
     1321 gacgcggcgg gcttttctgg cgggctcagc cgccgcggtt tccttttttg ggaagagact
     1381 tccggcagag gttgagggag tgccaatccg agcgataacc aagggcccgg gattccattg
     1441 gtttgcttat tatgacaagt ttcagttcga tccaacggac cgatatgcac ttggtatgaa
     1501 agtgctattc gagcacagat caccaacgcc ccaggacagc attgaaattg gcatgattga
     1561 cctggccgag ggtgaccgct ggattccgct aggcgagacc acggcgtggt gttggcagca
     1621 aggttgcatg ttgcaatggc gcccgggctc ggccaccgag atcgtctata atggccgggg
     1681 t
//
LOCUS       DAOPTR010000948         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_56182_length_1680_cov_3.130462, whole genome shotgun sequence.
ACCESSION   DAOPTR010000948 DAOPTR010000000
VERSION     DAOPTR010000948.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56182_length_1680_cov_3.130462"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="PKI05_14790"
     CDS             <1..565
                     /locus_tag="PKI05_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53518.1"
                     /translation="EYGATWVSVEAPLVELGEIRMDLSNPFDPSTWVTELEPTQTLLS
                     YVMNNYWETNYKADQEGPTPFVYYVRGHQGPCDPTAAARFGMERSRPLVAVFCQENAA
                     KEIPSLISLNSPKVLPVECKPTRNGTGIIVRLFNFGDQPEEVTLGPGGQAKWKEIWRT
                     DLSETKKEAIGGKILIKPRELVSLRLE"
     gene            complement(617..1324)
                     /locus_tag="PKI05_14795"
     CDS             complement(617..1324)
                     /locus_tag="PKI05_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53519.1"
                     /translation="MPLTVRCPSGHAFLAAPNFVGREVRCPYCQQMTVVEVAVSEHQT
                     SPAKPPSPGAAAGGEPSAPPNFAQGDPTSAKIIDPVINEVASKAPTPRLSFGMVLGLL
                     AVAMSSLIPAALAIAQNCGGRWPTGVVAISALQIGAVFLLVILPDWTSLKIVGLLFGI
                     AAALFGMGAAFLAFAVESRLHAMGIESTLRDAAARWMISLMAIQATTTFLCFRAAETW
                     RRRVSIIFARKPRRATT"
BASE COUNT          405 a          447 c          485 g          343 t
ORIGIN      
        1 agagtatgga gcaacctggg tttccgtgga ggcaccgctt gtggaattag gcgaaatccg
       61 catggatctc agcaatccct ttgatccgag cacctgggtg accgaactgg agcccacgca
      121 aacgctgctc tcttacgtga tgaacaacta ctgggagaca aactacaagg ccgatcaaga
      181 ggggccgacg ccctttgttt actatgtccg ggggcaccaa ggcccttgtg acccgaccgc
      241 cgccgcccgc tttggcatgg aacgaagtcg ccccctcgtg gccgtgtttt gtcaggaaaa
      301 cgctgcgaaa gagatacctt ctttaatctc actaaattcg cccaaggtgt taccggttga
      361 gtgcaaaccc acccgcaatg ggacggggat tatcgtgcgt ctcttcaatt ttggagacca
      421 accagaggag gttactcttg gccccggtgg ccaggcgaag tggaaggaga tctggcgaac
      481 cgatctgagc gaaaccaaaa aagaggcaat tggtggaaag attctgatca aacccagaga
      541 gttagtctcg ttacgcctgg agtaaccaag cgacttgtga ttggcaagga aaggacctgc
      601 tggtgtagct tggaagtcat gtggtagccc gtcgtggttt tcgcgcaaat ataatgctga
      661 cgcgacgtcg ccaggtctct gccgcgcgga agcagaggaa cgttgtggtg gcctggatgg
      721 ccatgaggga tatcatccat cgggctgctg catctcgtag ggtactttcg atgcccatcg
      781 catgaagtcg cgattccacc gcaaaggcca gaaatgccgc gcccatgcca aagagggccg
      841 ccgcgatccc aaaaagaagc cccacaattt tcagagaagt ccagtccggc aggatcacca
      901 ggagaaagac cgcgccaatt tgaagggccg aaatcgccac tactcccgtt ggccaacgtc
      961 ctccacagtt ctgagcgatg gcaagtgcgg ccgggatgag actgctcatg gccactgcca
     1021 acagtcctag caccataccg aacgacaacc gaggggtggg agcctttgaa gcgacttcat
     1081 taattaccgg atcgatgatc ttggccgagg ttgggtcacc ttgagcaaaa tttggcgggg
     1141 cggatggctc gccaccagcg gcagctcccg gtgaaggagg tttcgcgggg gaggtctggt
     1201 gctcactaac cgcgacttcc acaaccgtca tttgctggca atacgggcat cgaacctctc
     1261 gacccacgaa gttaggggct gcaaggaatg cgtgtcccga tgggcagcga accgtcaacg
     1321 gcatggcagt ctcattggct aggtcaacat gagaaatccc ctgacaccgc tcagtttaat
     1381 cggcgtggcg agggcttcca acgacgtcgg cttaagaaat ccaccgatct ttttccaaca
     1441 agacacataa attggcccac gcggcaccgg ggataccctt tgccggataa agttgcccgg
     1501 gaaataattg ggaatgcatg gtagccctaa aagtggattc agccgtcctg tgatgtgatt
     1561 gagggaccat gctacaaaaa ggtcatctcg tttatgggaa taatgcagtt tcaggtttcc
     1621 gatgaggccc cgacgccaga cgctctggag aacttagcct atttggccgg ctttgagcgg
//
LOCUS       DAOPTR010000949         1679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_56220_length_1679_cov_4.012315, whole genome shotgun sequence.
ACCESSION   DAOPTR010000949 DAOPTR010000000
VERSION     DAOPTR010000949.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56220_length_1679_cov_4.012315"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PKI05_14800"
     CDS             complement(<1..369)
                     /locus_tag="PKI05_14800"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOA53520.1"
                     /translation="MKTPVLQILCRIFLWLVLCGSAGEVCAWLWSTGTPATPHPAVAR
                     IFALDTRAVFKGTGTLVAVSSYHGLVITNWHVVRDAKDAVLVQFPDGFASPATILKVD
                     RDWDLAALAIWRPRAKPVPIA"
     gene            1452..>1679
                     /locus_tag="PKI05_14805"
     CDS             1452..>1679
                     /locus_tag="PKI05_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOA53521.1"
                     /translation="MRILFASSEAVPFATTGGLGDVCGSLPFALANLGHDVRVIIPAY
                     RGIWYAGREIRSLGLEFIVSVGSKTVSGRLLE"
BASE COUNT          412 a          463 c          440 g          364 t
ORIGIN      
        1 ggcgatcggt accggtttgg cacgtggtcg ccagatagcc agcgccgcca agtcccaatc
       61 gcgatcgacc ttcaagatgg tcgctgggga ggcgaaaccg tcgggaaatt gcaccaatac
      121 ggcgtccttg gcatctcgca caacatgcca attggtgatc acaaggccgt gataagagct
      181 gaccgcgacc agcgttccag tgcccttgaa aactgctcgg gtgtccaagg caaaaatgcg
      241 ggccacggcg ggatggggcg tcgctggtgt gcctgttgac caaagccagg cacaaacctc
      301 ccccgcgctc ccacaaagga cgagccacag gaagattcga caaaggattt gaaggaccgg
      361 cgttttcaca ttacgcaaat gtcgcgagag ttgaagctat catcggacaa aaaacattca
      421 tgggtatcct gaacgactta aaccctggac acgcatccca aacgggtcaa accaatgggg
      481 acaaatgtcc gttacgacaa ccgcaaactg ctgccgcttt aacagatctg cgaattgatc
      541 acttcaccgt ttggtgaatc agcgagccgt aatgccgctt tattgtcaaa tcaatcaagg
      601 tgccatccct gcggcatccg tgctcctcca cagccatgag cggaggaaaa gaaccatcct
      661 gtggcgagac ttttctgacc ggggcaagac cccttcatct agcattcggc ataaggaggg
      721 cgttgcccga agtttctttt tgtcaacatt cccgaacaca aaatgagcat gcctcccgtg
      781 gtccgcaaga ccaaaatctg actcacaaca cgtccaagga aaacgactga aatcacccgt
      841 ttctccccgt cagctaatac atcgcatagg ctacttgtct tcgatatgac ccgaacgaca
      901 tgaagcactc tcttggtgac gacatttccg caaatcaata ccggttgaga agataagcct
      961 cgaccgagag cctatgccaa aaagtccccc tctccctctg ggagagggtt ggggtgaggg
     1021 caaacgaatg tgggaatcga ttgagaaaac cggcagcgat tccagccacc ctcacccccg
     1081 gcccctctcc tgccgaacgg gcgaggggag attatgggat aggctctaag ccgtatcgct
     1141 gcgatattgg ccccacgccg gtccgttttc cctttggaaa ggagccttgt tcgtcatcca
     1201 acgtcaccac aggcagccgt aaaattcaga tgaatctggt atatttgatg gttttatgag
     1261 ttgaaagcga agtgcccatt tgtatcgaca aaaacttcct tccccgaaat cctgagctag
     1321 acttaatggg gtatccctca gcgccagaat actgttaagg gtttctgcct tgttgtggcg
     1381 actgggcaaa atgttttgcc gagtgattcg gcatgctctc taagaccgta agccgtccgg
     1441 aggtgacgcc agtgaggatt ctatttgcaa gcagtgaagc cgtgcctttc gcaaccaccg
     1501 ggggattagg ggatgtctgc ggcagccttc cctttgcact cgccaacctg ggacacgatg
     1561 tccgcgtgat catacccgct taccgcggca tttggtatgc gggtcgggaa atccgttcgc
     1621 ttgggcttga gttcattgtt tcagtgggaa gcaagaccgt ctcgggcaga ctgctcgaa
//
LOCUS       DAOPTR010000950         1679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_56244_length_1679_cov_2.556034, whole genome shotgun sequence.
ACCESSION   DAOPTR010000950 DAOPTR010000000
VERSION     DAOPTR010000950.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56244_length_1679_cov_2.556034"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="PKI05_14810"
     CDS             complement(<1..779)
                     /locus_tag="PKI05_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53522.1"
                     /translation="MKALHDPLSMDRAVFAFRRRMICFLIAAICLAEIQYLTMGALAV
                     EVGKSLAPIRSARVRTIDGIPRLVLNGQPVPARIFWGAPGRGAIQAGPTGQSVTFEFV
                     AYDDAERQGTLHFRFGRLPGSVLLDSIRVQDVDTGDTILSCDFEDEQDFSANWSIWPP
                     DERNTVGHVEILSDSGENESGCLAIHLNKPPQGEWPDFHIYSRPSLPIVKGRRYRVKL
                     WVQSHVPRKVIIAFYRPGSPFVLLGGPPGPFRSQVRLAAQSG"
     gene            1347..>1679
                     /locus_tag="PKI05_14815"
     CDS             1347..>1679
                     /locus_tag="PKI05_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53523.1"
                     /translation="MKRLFTGILFISAALLVLGSVTGCKVAMSTGPIGVEAVKLNPEE
                     WNGIWQDPVNNGFVAVRVADADQGILEAAGIETRDDGLELKKLRLHVRQANSWMFLSL
                     ESPDRPGNY"
BASE COUNT          394 a          441 c          491 g          353 t
ORIGIN      
        1 ccagattgag cagcaagacg tacctgtgac cgaaacggtc cgggcgggcc gcccagtagt
       61 acgaacggtg aacccggacg gtaaaacgca attatcactt ttcggggaac atgggattgt
      121 acccagagct ttacgcggta acgtcgtccc ttcacaatcg gcaaagaagg gcgcgagtag
      181 atgtgaaaat ccggccactc accctgtggg ggcttattga gatgaatggc caagcagccg
      241 ctctcgtttt ctccagagtc cgaaagtatt tccacatgcc ccactgtgtt tcgttcgtcc
      301 gggggccaga tggaccaatt cgccgaaaaa tcttgctcgt cctcaaaatc gcaggaaagg
      361 atcgtgtcgc ccgtgtcaac atcctggact cgaatactgt cgagtaggac ggaaccgggc
      421 agcctcccaa agcggaagtg gagcgtcccc tgacgttccg cgtcatcgta ggcaacgaac
      481 tcaaaagtca ccgattgacc tgttggtcct gcctggatag cgccccggcc cggcgcaccc
      541 caaaagattc gggccggaac aggctgacca ttcaacacaa gcctcggaat gccatcgatg
      601 gtacgaacac gtgcactccg gattggagcc agcgatttgc cgacctcgac ggcgagcgct
      661 cccatagtca agtattggat ttccgcgagg catatggcgg cgatcaaaaa gcaaatcatt
      721 cgacggcgga atgcgaacac cgccctgtcc atcgaaagtg gatcatgtag ggccttcacg
      781 ggagatgctc ctctttcatt tgcatagaac agttgacaaa tatcgctgag atcttggtct
      841 gctgcccgta aaaaccacag gcgttgccac gattatgggc cagccgcgcg tgtctgtcca
      901 gagaccggta cggagccagg ctcgatcccc gatgagcgcg gggaagcttt gtgcggctaa
      961 ctcaacaatc cgagtagttg catatgtgat taggtggcag cgcctggcca taccggggca
     1021 atgaccgggg aggtcacgac ggagcaaagt gccataaacc cagcttttct cgaagcccga
     1081 cttctgttgc tcagcatgac cagccgtcct atatcaaagg ggagggatca aatcgccctc
     1141 ggagcttggc aaggctcact atggtcaact gcggataccg ccagggttat gggtaagtcc
     1201 cgtcggcccg ggcaaggacg gtcatgagat tgggggttaa tgacagaggt gggcagaacg
     1261 tttctcgcag ccaaaataaa gcatataata cggtgaagcg ggtgtgtcgt gtgcatgttg
     1321 gtcgtcttgg cccaggagac acgattatga aacgactttt cacagggatc ctcttcattt
     1381 ccgcagcttt gttagtcttg gggagtgtga ctggttgcaa ggtggcgatg tcaaccggtc
     1441 cgatcggagt cgaggcggtc aagcttaatc ccgaggagtg gaatggcatt tggcaagatc
     1501 cggtcaacaa tgggttcgtt gctgtgcgtg tggcggacgc agatcaaggg attctcgagg
     1561 cggcaggcat cgagacacgc gatgacggct tggaattgaa gaagcttcgt ctccatgtcc
     1621 gccaagctaa cagctggatg tttctttcct tggaatctcc cgatcgtccg ggaaactac
//
LOCUS       DAOPTR010000951         1676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_56357_length_1676_cov_4.222085, whole genome shotgun sequence.
ACCESSION   DAOPTR010000951 DAOPTR010000000
VERSION     DAOPTR010000951.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56357_length_1676_cov_4.222085"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="PKI05_14820"
     CDS             complement(<1..790)
                     /locus_tag="PKI05_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOA53524.1"
                     /translation="MKAPFGRCERSFDKSGDRRKGDLVWTVLWGLFLVWEAAVGFGQD
                     VAPVHHVPWLGDVQMGLALGNDQNIPMNLLVRPDGSRIARAEQWRAYREGIRKWWLEF
                     LKLPERDIHRSLATMQVMEKAETDGVVRCLIRYETPGNWPTEAFLLFPKDTNPLESNK
                     TLLPAVIVFHSTTNDTIRQPAGLTAETTKAFGLQLAKRGYVTLCPRCYLWSDGPPYDY
                     QAQVRRYQRSFPGSKGMGKMLADGMAAVDLLTQLPGVDGQRIGAI"
     gene            complement(994..>1676)
                     /locus_tag="PKI05_14825"
     CDS             complement(994..>1676)
                     /locus_tag="PKI05_14825"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA53525.1"
                     /translation="NEPWKGKSGLNVYADFVMETDAMVGRVLDTLDEIGVTDKTLVIF
                     TSDNGKARYTGAEALEKMGHFSSGPFRDYKASVYEGGHRVPFIVRWPKVVPPGSVCIQ
                     LVHQADLFATLAEILEVPIPPNAGEDSFSLLPLFRGENRSVRPHAVSCASSGVPSVRE
                     GPWKLILNKNPELYQLDNDPGETANLADRYSDRVEAMKKLLEKIIRDGRSTPGPQQKN
                     DVPVKRYP"
BASE COUNT          392 a          510 c          399 g          375 t
ORIGIN      
        1 caatggcccc gatgcgttgc ccatcaacac ctggcaactg ggtcagcaaa tcaaccgccg
       61 ccatcccgtc cgctaacatc ttgcccatcc ccttcgaacc gggaaatgac cgttgatagc
      121 ggcgaacctg tgcctggtaa tcatacggcg gtccatcact ccacaaataa caacgcggac
      181 ataaggtcac atatccccgc ttggccagtt gaaggccaaa ggccttcgtt gtttccgccg
      241 ttagcccggc cggttggcgg attgtgtcat tcgtcgtgga gtgaaaaacg atcaccgccg
      301 gcaaaagggt cttgttcgat tctaaaggat tggtgtcctt agggaagaga agaaacgcct
      361 ctgtgggcca attacccggc gtttcgtaac gaatcaaaca ccgcaccacg ccatccgtct
      421 ccgccttctc catgacctgc atcgtggcca aactgcggtg gatatcacgc tcaggcaact
      481 tcagaaactc caaccaccac ttgcgaatac cttcacgata tgcccgccac tgctcggccc
      541 tcgcaatgcg agagccatcc ggcctgacga gcaagttcat gggaatgttt tgatcattgc
      601 ccaaagccag tcccatctga acatcgccga gccaagggac atgatgaacc ggcgctacgt
      661 cctggccaaa acctaccgcc gcttcccata ccagaaaaag accccataaa accgtccaaa
      721 ccaaatcgcc tttacgtctg tctccgcttt tatcgaaact tctctcgcac cggccaaatg
      781 gggctttcac ggctgcgcct ccttaaatag gacgacgact ctcgagcatc ggacggcccg
      841 aaaacatctc gctggtgccg gagctctctt tccaaggcat caaccgacgt gttattcagc
      901 gatagctgtg cagaatcttc tgacggtaat gataaaatct agccaaaacg atcttacacc
      961 aaatctttgg ccattccctt tggcaataac tgcctacgga tatcgcttca ctgggacgtc
     1021 gtttttttgt tgagggccag gcgtacttcg gccatctcga ataatctttt ctaaaagttt
     1081 tttcatggcc tcaacgcgat cggaataacg gtcggccaga ttggcggttt cgcccggatc
     1141 attgtcgagc tgatatagct cagggttttt gttcaagatt agtttccacg gcccctcgcg
     1201 gacgctaggg accccgctgc tagcacaact gaccgcatgg ggccggacag accgattctc
     1261 tccccgaaac agcggcaaaa gactgaagct atcctctcca gcgttgggtg ggatgggaac
     1321 ctctaggatc tcggccaacg tggcaaagag gtcggcttga tgcaccaatt gaatgcacac
     1381 actgccgggc ggcacgacct tcggccagcg aacgatgaac ggaacacgat ggcccccttc
     1441 gtacaccgat gccttatagt cccggaaagg tccactggaa aaatggccca tcttttccag
     1501 tgcttcggcc cctgtatagc gggccttgcc attgtcactg gtgaagatga caagggtttt
     1561 gtcggtcacc ccgatttcgt cgagggtatc cagcacacgc ccgaccatgg catccgtctc
     1621 catcacgaaa tccgcataca cgttgaggcc actttttccc ttccatggtt cattca
//
LOCUS       DAOPTR010000952         1675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_56401_length_1675_cov_3.804321, whole genome shotgun sequence.
ACCESSION   DAOPTR010000952 DAOPTR010000000
VERSION     DAOPTR010000952.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56401_length_1675_cov_3.804321"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            345..1460
                     /locus_tag="PKI05_14830"
     CDS             345..1460
                     /locus_tag="PKI05_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008682751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA53526.1"
                     /translation="MARDRVRVAIIGLGFGAEFIPIYQRHPQAEMYAICQRNQERLHQ
                     VGDIFGVPVRYHRYEDVLADPNVDFVHINTPIPDHAWMSIEALKAGKHVMCTVPMATT
                     IDECRKIVELVHETKLKYMMAETVVYSREFLFIKEMYQKGELGRIQYLQASHTQDMDG
                     WPDYWQRMVPMHYATHVVGPCLGLLNGRAEYVSCFGSGRVRQEIWEKSGSPFAIESCH
                     IKIKDSDVVAHIWRFLYDTARQYRESFDVYGSRASFEWTLVEGEPHIWHTAKKPEPEI
                     PSKIRVPDYAHLLPEPIRLFTQAIHDPQHRSFIQGAGHGGSHPHLVHEFVSALIEDRD
                     PWPNAVQSANWTCVGICAHQSAMEGGRIIPLPDFTLT"
BASE COUNT          364 a          452 c          478 g          381 t
ORIGIN      
        1 cttcgggcga cttggtgggg tcaaggcttg gtagcaagcg gctcctctca aagtcggctt
       61 cctggccacg atccgcccaa cgtaatgcgc gcttacgagt gctggccgtt accgcacagc
      121 aattgtcccc aattgattgc acagcgcacc cggttcgcat acaatcgacg cagccgtcgg
      181 ggaaagactt ctgcgagttt cagcaagcgt gtccggcttc cttaggtttg catcaacggc
      241 tctggctgtg acaatttgct gagacgattt agcgggaacg ccgttctgtt tagagcatgg
      301 tgtggaaggg catcaaccct gttccaacaa taggagactc gtcgatggcc agagaccgtg
      361 ttcgagtggc aattattggg cttgggtttg gagccgagtt catccccatc tatcaaaggc
      421 acccgcaggc cgagatgtac gccatttgtc agcgtaacca ggaaagactg caccaagtgg
      481 gcgacatttt cggtgtcccc gtccgctatc atcggtacga ggatgttctc gccgacccca
      541 atgtggactt tgtccacatt aacacgccga ttcctgacca cgcttggatg tccatcgagg
      601 cactcaaggc gggaaagcac gtcatgtgca ccgtgccgat ggcaaccacc attgacgaat
      661 gtcggaagat tgtggagctt gttcacgaga cgaaactcaa gtacatgatg gccgagacgg
      721 tcgtctattc ccgcgaattc cttttcatca aggagatgta tcaaaaagga gaattgggca
      781 ggatccagta tctgcaagcc tcacacactc aggacatgga tggttggcct gattactggc
      841 agcggatggt ccccatgcac tatgccacgc acgtcgtggg gccgtgtttg ggcctgctca
      901 atgggcgggc tgaatatgta tcgtgtttcg ggtcagggag ggttcgtcag gagatttggg
      961 aaaaatcggg cagtccgttc gccatcgaat cgtgtcacat caagatcaaa gactcggatg
     1021 tggtggccca catttggcgg ttcctctacg acaccgctcg gcagtatcga gaaagcttcg
     1081 atgtctatgg aagtagggcc agttttgagt ggacactggt cgagggtgag ccacacatct
     1141 ggcatacagc caaaaagcca gagccagaga ttcccagcaa gatcagggta cccgattacg
     1201 cccatttgtt gccggaaccc atccgcctat tcacgcaagc gattcacgat ccgcaacatc
     1261 gttcctttat ccagggggcg ggtcatgggg gatctcaccc ccatttggtc cacgaatttg
     1321 tttcggccct catcgaggat cgcgacccct ggccgaatgc cgtgcagtcg gcgaactgga
     1381 catgtgtggg catctgcgcg caccaatcgg ccatggaagg aggacggatc atcccgctgc
     1441 ccgacttcac actcacctaa ccggtggctt tcgtgaaggt ccggttttgg tcaagatgat
     1501 gcgaaaccgt tttaatggcc cgcatcctgt tatgtaggat ttccatggtt ttgaacggtc
     1561 tagcagacta agccccttct cgattgcaaa gccgtggtgg gcccgctgat cggagttcta
     1621 gcctccgcgg ccggtggcgg cctagcacag aatcggcaaa ctgggtccgg atctt
//
LOCUS       DAOPTR010000953         1674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_56458_length_1674_cov_2.515750, whole genome shotgun sequence.
ACCESSION   DAOPTR010000953 DAOPTR010000000
VERSION     DAOPTR010000953.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56458_length_1674_cov_2.515750"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..270)
                     /locus_tag="PKI05_14835"
     CDS             complement(10..270)
                     /locus_tag="PKI05_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53527.1"
                     /translation="MKEFFECEEVERLVATGNTESIVAGRTCRLLRLSLESIEVLMNT
                     QPEAQVESLRPAINRFCQSQLPPHVKEVAFRVRRKMQDHTGE"
     gene            complement(303..1172)
                     /locus_tag="PKI05_14840"
     CDS             complement(303..1172)
                     /locus_tag="PKI05_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53528.1"
                     /translation="MSYDDRAIVTGDEKEDTVALKPVSAGAEGQGGKPMDPHAEAARD
                     TAQSTAPAVRHLRVDGAKHPPGGLSQNLMWLIFSRIGVPLLMLAGAVLLLQKARLWFR
                     PPLEMPQIIQDIRSDGAARWRAVAQIGAIMADGRYPEVRSDRELAMAMSDAFREELQR
                     PLLSVSDLSVVSRKYLCFLLWQMDDESVILALCEGATWWGISMPQPGSPVRCAALEGL
                     CEMASGLARRSSGRVRRSCLHCVKRPGIRLIRSGRMRHSPWDCTAVLADVKHWKGSSM
                     IPHGMFVTAQPLG"
     gene            1337..>1674
                     /locus_tag="PKI05_14845"
     CDS             1337..>1674
                     /locus_tag="PKI05_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53529.1"
                     /translation="METFFRNEFFEHCNGLVIGMDFLDLTLPQIQANLALDEALLHAA
                     DAGELGEVLRIWEVPSFAVVIGRGSRVAEEVHKETCERLGVPIVRRCSGGCAVVIGPG
                     CLLYSLVFDL"
BASE COUNT          355 a          514 c          442 g          363 t
ORIGIN      
        1 tatcctcgct cactccccgg tgtggtcttg catttttctt cgcacccgaa aggcgacctc
       61 tttgacgtga ggaggaagct ggctttggca aaaccggttg atggccggtc gcaagctctc
      121 cacttgagct tcgggttggg tattcataag tacttcgatt gattccaagc tcaagcgtaa
      181 cagtcggcat gtccgaccag ccacgataga ttccgtgttg cccgtggcca ccaggcgttc
      241 cacttcttcg cactcgaaaa attctttcaa ggtgggcaca cccttgtcac ttcctgccgt
      301 actcagccca atggctgcgc tgtaacgaac attccgtgag ggatcattga ggagcccttc
      361 caatgtttca catccgctag gaccgccgtg cagtcccaag gcgagtgccg cattcgcccg
      421 gaccggatca atcggatccc tggccgcttg acgcaatgca ggcaggaccg ccggactctg
      481 ccggaggacc tccgggccaa gccgctggcc atttcgcaca aaccttctaa agccgcgcac
      541 cgaacgggcg atcccggctg gggcatcgat atcccccacc aggtcgcacc ttcacacaat
      601 gccagaataa cactctcgtc atccatctgc cataggagaa aacaaagata cttccgcgag
      661 accaccgaca aatcggacac actaaggagc gggcgctgca actcctctcg aaaggcatcg
      721 ctcatcgcca tggccagctc cctgtcgctt cgtacctccg gataccggcc gtccgccata
      781 atcgcgccaa tctgggccac ggcgcgccat cgggcagctc catcggagcg aatatcctga
      841 atgatctgag gcatctccaa agggggcctg aaccacaacc gcgccttttg cagaagtaaa
      901 actgctccag caagcatgag gagtggcacc ccaatgcggg aaaaaatcaa ccacatcagg
      961 ttttgcgaca acccgcctgg tgggtgtttt gctccatcaa cacgcaagtg acgaacggca
     1021 ggagccgtgc tttgggccgt atcgcgggct gcttcagcat gcggatccat gggcttgccg
     1081 ccttggcctt ccgcgccagc agagaccggt ttcaaggcaa cagtgtcctc cttctcatcc
     1141 cccgtcacga tggcccgatc gtcgtaactc atcaccccac ctcctttcgc catttgcctg
     1201 attgacaagg cacattgtgg gcggtatttt cacccccaca ttcaaaagag cgcgactcac
     1261 ggcgaaaagc ctcgcgatcc caaccgacgt attttatggc ccatgggacg acctgtgtca
     1321 agcaaaatcc ggcgagatgg aaacgttttt ccgtaatgag ttctttgagc actgcaacgg
     1381 gttagtaatt ggcatggact ttctcgatct gacgctccca cagattcagg ccaacctggc
     1441 tttggatgag gcactccttc atgccgccga tgccggggag ttgggagagg tccttcgcat
     1501 ttgggaggtt cccagttttg ccgtcgtgat cggccgtggc tcgcgggtag cagaagaggt
     1561 ccacaaagaa acatgcgaga gactgggcgt ccccattgtc cgtcggtgta gcggaggatg
     1621 tgccgtcgtg atcggtcccg gctgtctttt atattccttg gtgttcgact tgaa
//
LOCUS       DAOPTR010000954         1671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_56597_length_1671_cov_3.429455, whole genome shotgun sequence.
ACCESSION   DAOPTR010000954 DAOPTR010000000
VERSION     DAOPTR010000954.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56597_length_1671_cov_3.429455"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1637
                     /locus_tag="PKI05_14850"
     CDS             <1..1637
                     /locus_tag="PKI05_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53530.1"
                     /translation="LSEQQVVRGADPLPIGVTVPGFFLSEETDGKAASFVPAEARLEP
                     IYAETPAAPKTAINWKNLTFVSFLENASNENGKVGQQASSLAERESAAGESQPSVGEA
                     NQERPQQQTRSQRRRGSRASQEGGEQPEESSTALPMPATPAISGWRIIVESPQVTSPQ
                     SVKYVMPKKRAGVICDENFVPLQSFQASITDGPRAMRSPIGFWHIVFFAWICNLAMHL
                     GLSDMAIFRFAKRSWYGLYSAFGMFLGHYIAWICAGIMGAAAARLTMTPLMQLDSGQV
                     AYLILGWAGVVAVVIAGLTTSNPTLYRAGLALQILTPNWPRWVVTLLAGTITTIIACS
                     PFVFIYLLDFVGIYGILLMPVGAIVVVEHWVFPLLGWRRFWATYRGQWLNIPAILAWG
                     VGLGLALVCWKFQWLHLFFLVIPVWFATALTYLILAGLGGAARKTEGETSPTTPSSDS
                     AGRESTPASTFAGEKKNLDPGTDTPGRRPLLMYAFGFAAMVCLLGSYAFAWFVFAGYS
                     TAEDVKPTLLYLALAYFVSGVIYLSLRERRTATSAA"
BASE COUNT          335 a          439 c          461 g          436 t
ORIGIN      
        1 ttcttagcga acagcaggtg gtaagggggg cagacccatt acccattggt gtcactgtcc
       61 ccggtttctt cttgtcggaa gaaactgacg gaaaagccgc gtcttttgtc ccggctgagg
      121 ctcgtcttga accgatttat gcggagactc cggcagcccc caagaccgcc attaattgga
      181 aaaatctaac tttcgttagt tttttggaaa atgcctcgaa cgaaaacggg aaagtcggcc
      241 agcaagctag cagcttggcg gagagggaga gcgctgccgg cgaatcccag ccttcggtcg
      301 gggaagctaa ccaagagcgg ccgcaacaac agacacggtc tcagcgccgc cgcggttcca
      361 gagcatctca ggaaggcgga gaacaacctg aggagtcaag cacagccctt ccgatgccgg
      421 cgacgcctgc catttctggt tggcgaatca ttgtggaaag tcctcaggtc actagtcccc
      481 aatccgtgaa gtacgtcatg ccgaagaaac gtgccggagt gatctgcgat gaaaactttg
      541 tacccttaca gagttttcag gcttccatta cagatggccc tcgagcgatg cgttcgccga
      601 ttggtttttg gcatattgtt ttctttgctt ggatttgtaa tctggctatg catctgggct
      661 tgtccgacat ggccatcttc cgattcgcta agcgttcgtg gtacgggctt tattccgctt
      721 tcgggatgtt tctgggacac tacatcgcct ggatttgcgc ggggatcatg ggagccgcgg
      781 cagctcggtt gacaatgacg ccccttatgc aacttgattc gggacaggtt gcttacctta
      841 tccttggctg ggcaggcgtg gtggccgttg ttatcgccgg tttgacaaca tccaatccca
      901 cgctctaccg tgccggtctc gcgctacaga ttctcacgcc caattggccg cgttgggtcg
      961 taacccttct ggctgggacg attactacta tcattgcctg ttctccgttt gtgtttatct
     1021 accttctcga tttcgtcggg atctacggta ttttgctcat gcctgtggga gcaattgtcg
     1081 tggttgagca ttgggtgttt ccgctactcg gctggcgacg tttttgggca acctaccgag
     1141 gacaatggct caacatccct gcgatattgg cctggggtgt tggacttggc cttgccctag
     1201 tttgttggaa gttccagtgg ctccacctat tctttttggt cattcccgtt tggtttgcaa
     1261 cagcgttgac atatctgatc ttggccggac tgggaggggc cgccaggaaa acggagggcg
     1321 aaacgtcccc aaccacgccg tcaagtgatt ccgctggccg ggaaagcaca cctgctagca
     1381 catttgctgg agaaaagaaa aatctcgatc caggtacgga tactccaggc cgtaggccgc
     1441 ttctcatgta tgcttttggc tttgcagcca tggtttgcct actcgggagc tacgcctttg
     1501 catggttcgt attcgcgggg tattccacag ccgaagacgt gaaaccaaca ctgttatatc
     1561 tggcattggc gtactttgtc tctggcgtga tctatctctc gctgagggaa cgacgcaccg
     1621 caaccagcgc agcgtaagcc aatgtcagcc attaaagagg tttctttcat g
//
LOCUS       DAOPTR010000955         1670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_56650_length_1670_cov_2.806192, whole genome shotgun sequence.
ACCESSION   DAOPTR010000955 DAOPTR010000000
VERSION     DAOPTR010000955.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56650_length_1670_cov_2.806192"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..1447
                     /locus_tag="PKI05_14855"
     CDS             257..1447
                     /locus_tag="PKI05_14855"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOA53531.1"
                     /translation="MRRLTAYALLIVILLCVILGDGPVGTIAPRDAFAQQSPGRTLLV
                     AVDRYGPLDEQTRAELRRQLEETGKVLEAQSAAMRLVAKLVGPAVVHIEAQTIGRRAL
                     QHGAARPVEEAGSGVLVKLGEKTCVLTNRHVIAGATPDKVRISLADGRVIQPQQIWSD
                     TESDVAVLEVGISDVVTAILGNSDTVDIGDFVLAFGSPFGLSHSVTFGIISGKGRRDL
                     DLGDSGVKFQDFLQTDAAINPGNSGGPLVNLKGEVIGINTAIASSSGGNEGVGFAIPI
                     NTFLFTARQLVEKGAVTRAFLGVTLDARFTIEAAQAAGLQQRIGARVVTVIPDSPAAQ
                     AGIQAGDIILRYDTTLVESDGHLINLVNTSDVGKEVTLEIVRAGQVQKVKVVLTPKPA
                     TPAN"
BASE COUNT          353 a          413 c          530 g          374 t
ORIGIN      
        1 gccggcatga acgagacaaa ctttcggcac gtttaggtgc tggccgtggg tgggctataa
       61 ttgagggagc ccattcaacg ggcgccgcgc agaaattgga tcacgggccc aatggatggt
      121 cggagcgacc agtctggaat gatcatcccg ggaaaaaggg gccttcttgg ggctgggttg
      181 tttaatgaaa aagggggcat cgtatggtgg ccattggcac aagaaagctt tgatctacac
      241 ggtcatgaga acccatttga gacgattaac ggcttacgca cttctgatcg ttatcctttt
      301 gtgtgtcatc cttggggacg ggccagttgg tacgattgcg ccgcgggacg cgtttgccca
      361 acagagtccg gggcggacgc tcctggtagc ggtggatcgt tacggtcctt tggatgagca
      421 aacgcgggcc gagttgcggc ggcaattgga agaaaccgga aaagttctcg aggcccaatc
      481 ggcagcgatg cgactggtgg ccaaactggt cggtccggct gtggtgcaca ttgaggccca
      541 aacgatcggg cggcgggcct tgcagcatgg cgcagcccga ccggtcgagg aagccggttc
      601 cggcgtcttg gtcaagctcg gtgaaaaaac gtgtgtcctg accaatcgtc acgtcattgc
      661 cggggctact ccggataagg tgcggatctc attggccgac ggaagggtga ttcagcccca
      721 gcaaatttgg tcggatacgg aatctgacgt ggccgtgttg gaagtcggga tttctgacgt
      781 cgtcacggca atcctgggga atagcgacac cgttgacatc ggtgacttcg ttttggcgtt
      841 tggaagccct tttggtctga gccattcggt gacctttggg attatcagcg gtaaggggcg
      901 aagagatctc gaccttgggg actcgggcgt gaaatttcaa gactttttgc agactgatgc
      961 cgctattaac ccggggaaca gtggcgggcc actggtcaat ttgaagggcg aagtgatcgg
     1021 cattaacacg gccattgcca gtagttcggg cggaaatgag ggcgttggtt ttgccattcc
     1081 catcaacaca ttccttttca cggcaaggca gttggtggag aaaggggctg taacgcgggc
     1141 gtttcttggg gtgacgctcg atgcgcgttt tactatcgaa gcagcccagg cagcgggact
     1201 tcagcaacgg attggtgccc gcgttgtgac ggtgatcccc gattcgcctg ccgcgcaggc
     1261 cggcattcaa gcgggcgata tcatcctccg ctacgatacg acgctcgtag agagtgacgg
     1321 tcaccttatc aacttggtta acacctctga cgtgggcaaa gaggtaacgc tggagatcgt
     1381 gcgggcgggg caggtccaaa aggtcaaggt tgtcctcacc ccaaagccgg ccacccccgc
     1441 caactgatcg cttaacggtc agctatgaca gatgcattgc ccaattccgg gccggattac
     1501 tgtgttgccg ctcttgccag tcgctgttcc aaaagttcaa acgtcttatc gcggtctatg
     1561 gaaaaaattc ggaccattcg accgtgggtg ttcttgtgat cgtataacat gtcccattgg
     1621 acgcttgggg catttacgac ttcgaactgc acaagggagg gatcgaccag
//
LOCUS       DAOPTR010000956         1670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_56662_length_1670_cov_2.495356, whole genome shotgun sequence.
ACCESSION   DAOPTR010000956 DAOPTR010000000
VERSION     DAOPTR010000956.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56662_length_1670_cov_2.495356"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="PKI05_14860"
     CDS             complement(<1..1172)
                     /locus_tag="PKI05_14860"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOA53532.1"
                     /translation="MGHILIVDDQPDICWGLKRIAEQLGHTAESVATAEEALSRVLST
                     NPDVVVMDVRLPGMTGLDAMQELKKKGWEGPVVVITAYGDVEVAVEAVRQGAFEYLTK
                     PFDLKVVARAIERAVASRLSTPTDEKTGELEERSWPQIVGVSPAMQQVFKQIAMVAPT
                     DAAVHIVGESGTGKELVARSIHGFSHRRKGPLVAAHIAALSPSLAESELFGHVRGAFT
                     GADYDHVGLLQRAHGGTLFIDEVAEIPLNLQVKLLRAIEYQEVTPVGAVRPIPCDFRI
                     ISATHRDLRKLVAEGKFRHDLYYRLITFEIRVPPLRERPEDIEPLTKFFLKELCRRME
                     KPVPHWTPEFMAALLSRPWWGNVRELRSALEHALILCRGGLLHPDLLPPPLPQASS"
     gene            complement(1217..>1670)
                     /locus_tag="PKI05_14865"
     CDS             complement(1217..>1670)
                     /locus_tag="PKI05_14865"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA53533.1"
                     /translation="KEPLDYAQVLESVLDLLRPAFQHAGVQLRVEMPQKPCVVTGHRA
                     SLEQLTTNLVMNALEAVQGVRDEPVVTVALRRSEEGVIQLVVEDNGPGPPANVAKDLL
                     KKPVTSKSDGIGLGLFVAQLIAEQHNGRIIWRRENGRTQFVFELPNHS"
BASE COUNT          365 a          486 c          434 g          385 t
ORIGIN      
        1 tgcgaggatg cctgcggaag gggaggtggt agcaggtcgg ggtggagtag tcctcctcga
       61 caaaggatca aggcgtgttc cagagcactt cggagttctc tgacattacc ccaccaggga
      121 cgactcaaaa gtgcagccat gaactctggg gtccaatggg ggaccggttt ctccatcctt
      181 cgacacaatt ccttgaggaa gaatttcgtt agtggttcta tgtcttccgg tcgttcccgt
      241 aaaggtggta cgcggatctc aaaggtgatc aaccgataat acaagtcgtg gcgaaacttc
      301 ccctccgcca cgagttttct gagatctcgg tgcgttgccg aaatgatgcg aaaatcgcaa
      361 ggaatcggac ggacagcccc gacaggtgtc acctcctgat attcaatggc acgtaacagt
      421 tttacctgca aattaagggg aatctcggca acctcatcga taaacagcgt tcccccgtgg
      481 gccctttgta aaagcccgac atggtcgtag tccgcacccg tgaacgcacc acgaacgtgt
      541 ccgaacaact cgctttccgc aagcgatggg ctcagggccg caatgtgggc ggcaacgagt
      601 ggacccttcc gccggtggct aaaaccgtgg atggaccgcg ccaccaattc ctttcccgtt
      661 ccactttcac ctacgatatg aacggcggca tcggtcgggg caaccatggc aatttgctta
      721 aagacttgtt gcatcgccgg cgagacccct acgatttgcg gccaacttct ttcctccaac
      781 tcgccggtct tttcgtctgt cggtgtagac aaacgagatg ccacagcccg ttcgatggct
      841 cgcgcaacaa cctttaggtc aaacggcttc gtcaggtact caaaggcgcc ctggcggacc
      901 gcctcgacgg cgacttcaac atctccatag gcggtaatta cgacaaccgg gccttcccaa
      961 ccttttttct ttagctcttg catggcgtcg aggcccgtca ttcccggcag ccggacatcc
     1021 atcacaacga cgtcggggtt cgttgataag acacggctaa gggcctcctc tgctgtggca
     1081 accgattcag ccgtatggcc gagctgttcg gcgatccttt tcagtcccca gcaaatatct
     1141 ggttgatcat ccacaatgag aatatgaccc atatctggcg aactccgtgg cgctccaagc
     1201 cccatctttt ggcgtgttaa gaatgattcg gaagctcgaa cacgaattgc gttcgaccgt
     1261 tttctcttct ccaaatgatt cttccattat gttgttcggc gataagctgt gcgacgaata
     1321 gtcccagccc aatcccgtct gatttcgagg tcacgggctt cttaagcagg tcctttgcca
     1381 catttgcagg cggtcccggg ccattgtctt cgaccacaag ttggatgaca ccttcctccg
     1441 aacgccgcag ggccacagta accacaggct catcacgtac cccttggacc gcctcaagag
     1501 cattcatgac aaggtttgtc gtcaattgtt caagcgaggc acgatgcccc gtgaccacac
     1561 atggtttttg gggcatttca acgcggagct gcacgccggc gtgctgaaag gctggccgaa
     1621 gcaggtcgag cacactttcg agcacctgcg catagtcgag aggttctttc
//
LOCUS       DAOPTR010000957         1668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_56745_length_1668_cov_2.676999, whole genome shotgun sequence.
ACCESSION   DAOPTR010000957 DAOPTR010000000
VERSION     DAOPTR010000957.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56745_length_1668_cov_2.676999"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PKI05_14870"
     CDS             <1..429
                     /locus_tag="PKI05_14870"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HOA53534.1"
                     /translation="NILACVEAKELGAQKVAAVVARSDYRYILTKLGVDHVTTPPDVV
                     RSEVEAHLTTGVVIAKKPFAPGSKVLVMELEVLPNTLITQHVLARVGLPRQCLIAALI
                     REDFAILPGADDRIKPGDTVVALVHESAVEAFLTFFEPAE"
     gene            521..>1668
                     /locus_tag="PKI05_14875"
     CDS             521..>1668
                     /locus_tag="PKI05_14875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOA53535.1"
                     /translation="MTSSRIYLDNAATTWPKPEAVYQAVDEYQRRIGCSPGRSTYADA
                     LAADRVVLEARLAVARWLRVGPADHVLFAFNGTDALNMALRGLIRPGDHVVTTIADHN
                     SVLRPLRWLAEQYGVRVDYVACDSEGFVELDALRSKLNRATRLVAVNHGSNVTGTLQR
                     LEAIVDVVRRHSSAMVLVDAAQSLGHVPLFPEEIGIDLLAAPGHKGLLGPAGTGILYV
                     RPGLEEELVPLRFGGTGSRSDEDRQPDEWPDRYEPGNPNTPALAGLIKGIEYLEQRGL
                     DDLRAHEVTLTQRLIDGLHAIPGVEVYGPKNAADRVGVVSINVKGLSPHELAAILDGQ
                     FHIQVRAGIHCAPLIHRRLGTLERGGAVRFSLGPFNTLEHVEAAIAAV"
BASE COUNT          327 a          482 c          494 g          365 t
ORIGIN      
        1 aacattttgg cttgcgttga ggccaaggaa ttgggtgccc agaaagtggc tgccgttgtg
       61 gcccgttccg attatcgtta catcctcacc aagcttggcg tggaccacgt aacgacgcca
      121 ccggatgtgg tgcgtagcga ggtggaagcc cacttgacca ccggcgtcgt gattgccaaa
      181 aaaccgtttg caccgggatc caaagtcctg gtgatggaat tggaagtcct ccccaacacg
      241 ctgatcacgc aacatgtcct tgcccgcgtg ggtctgcccc gccaatgcct cattgcggcc
      301 ttgattcgcg aagattttgc catcttgcct ggggccgatg atcgtatcaa accgggcgat
      361 acggtcgtgg ctttggttca cgaatcggct gttgaggcgt tcctcacctt ctttgaaccg
      421 gcagagtaac ctcgctcttg gcacccattg ggcggtctcc ccttgatccg gctccaataa
      481 ttgggcatgg tctcgcaaca gatcgttaga cagtttcaag atgacatcgt cacgcattta
      541 cctcgataac gcggcaacaa cttggcccaa gcctgaggct gtgtatcagg cagtggacga
      601 atatcaacga cggattggct gttcacctgg gcggagcacc tatgcggacg cccttgcggc
      661 ggatcgcgtt gtgctggagg cccgcctggc cgttgcgcga tggctgcgcg tcgggccggc
      721 tgatcacgtg cttttcgcct tcaacgggac ggatgcctta aacatggccc ttcgcgggct
      781 gattcgaccg ggagaccacg tcgtcacaac catagccgat cacaattcgg ttcttcgtcc
      841 cctccgctgg ttggcggaac agtacggtgt acgggtcgat tatgtggcct gcgattcaga
      901 gggctttgtg gaactggacg ccttgcgatc gaagctcaat cgcgcaaccc gtcttgtggc
      961 cgtgaatcac ggttccaatg tcacgggcac actgcagcgg ctagaggcca tcgtggacgt
     1021 cgttcgtcgg cattcctccg ccatggtttt ggtcgatgcg gctcaatccc ttgggcatgt
     1081 cccgctgttc ccagaagaga tcggtatcga ccttctcgct gcgccggggc acaagggact
     1141 gctgggacct gcggggaccg gcatccttta tgtgcgaccg ggtttggaag aagaactcgt
     1201 cccactccgg ttcgggggaa caggttcgcg gagcgacgaa gaccgccagc cggacgaatg
     1261 gcccgacaga tatgagccgg gaaatcccaa tacaccggct ttggcaggct tgatcaaagg
     1321 gatcgagtat ctggaacagc ggggcctgga cgacttgcgg gcacatgagg tcacgttgac
     1381 ccaacgcctc attgacggac tccacgccat ccctggggta gaagtctatg gccccaaaaa
     1441 tgcggcggac cgtgtcggcg tggtcagcat taacgttaaa ggtctgagtc cccacgaact
     1501 ggcggccatt ttagacggtc aattccatat ccaagtccgt gcgggcatcc attgtgcccc
     1561 cctcatccat cgtaggctag gtacgttaga acgcggcgga gccgtgcggt tcagccttgg
     1621 tcctttcaac acacttgaac acgtcgaagc agcgattgca gccgtcag
//
LOCUS       DAOPTR010000958         1668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_56750_length_1668_cov_2.117793, whole genome shotgun sequence.
ACCESSION   DAOPTR010000958 DAOPTR010000000
VERSION     DAOPTR010000958.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56750_length_1668_cov_2.117793"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PKI05_14880"
     CDS             <1..251
                     /locus_tag="PKI05_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOA53536.1"
                     /translation="RAMGIPKNMFTVLFALGRLPGWIAHWREMHLTPNKRIHRPRQLY
                     IGYPERPFVPLMKRSPKPLEEVSIKRPVGPFPGGEFEL"
     gene            complement(317..>1668)
                     /locus_tag="PKI05_14885"
     CDS             complement(317..>1668)
                     /locus_tag="PKI05_14885"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOA53537.1"
                     /translation="QVVGDAHQLYPATARPEFVLPTGRLVTVIDPMQAEAVPLASQVT
                     DDGANLPSVQARLSPSTNPPPVFPSVETWQVPSDDDQSRQVNLASQQEVVVTDESPLK
                     WSEGPAVQAATAKIERGSSVGSEVCEQVAVLAKVGGDVILAGEILGAVNEILLSYQDK
                     VPPSVLEQYKRDLMRRLLMQRVEMKLVYQDAKRHLPAEAVEHFEKEVSKLFDEKELPE
                     RLKKMGLNSPQEFDARLKKLGSSLAHEKRAFVEAVIAREWLRQQTEREANPLATASPE
                     ELLAYYQTHIQEFEKPGWVEWQQITVKKDRGRSPSEAAARAAEARDRVLKGEPFEDVA
                     RATSEGPTASSGGWRERTYRGSLRSKAIEDVLFSLPVGSISPIIEDEQGFHIVRVVRR
                     EETQRIPFSEAQVTIRDKLAQERRQQMTREWLRKLSQEIPVWTVYDQTGSPSNSVVR"
BASE COUNT          345 a          509 c          421 g          393 t
ORIGIN      
        1 atcgggcaat gggcattcca aagaatatgt tcactgtcct ttttgccctg gggcgtttgc
       61 ccggctggat tgcccactgg cgcgaaatgc atttgacccc gaacaagcgc atccaccgtc
      121 cgcggcaact gtacatcggc tatcccgagc ggccatttgt cccgctcatg aagcgatcgc
      181 caaagcccct ggaggaggtc tccatcaagc gccccgtcgg gccattccct ggtggcgagt
      241 tcgagctata gttcgtttgg acattccatt tctgcgaaca cgccgggaac tggattccac
      301 ctggagggca ggcggtctaa cgcacaacgc tattacttgg tgaaccagtc tgatcgtaaa
      361 ccgtccaaac tggaatttcc tggctcagtt tgcggagcca ctcccgggtc atctgctgcc
      421 gccgttcctg ggcaagtttg tcccggattg ttacctgcgc ctcactgaag ggaatccgct
      481 gcgtctcttc tcgccgaacc acacgaacga tgtgaaaacc ttgttcgtcc tcgatgatgg
      541 ggctaatact tcctacggga agggaaaaga gcacatcctc gatggccttt gaccgcaaac
      601 ttccacgata agtccgctca cgccatcccc ctgaacttgc agtcgggcct tccgacgtgg
      661 cacgggcaac gtcctcaaaa ggttcgcctt ttaagacccg gtccctcgcc tcggcggcgc
      721 gggcagcggc ttccgacggc gaccgaccac ggtccttctt cacggtgatt tgttgccatt
      781 ccacccatcc cggcttctca aattcctgga tatgtgtttg ataatatgcc agcaattcct
      841 ctggcgaagc cgtcgccagt ggatttgcct cacgctccgt ctgctgccgc aaccattctc
      901 gcgcgatcac agcctccaca aatgcccgct tttcatgggc cagagaacta cccaactttt
      961 tcagccgagc atcgaattct tgaggactgt taagtcccat ctttttcagg cgttctggta
     1021 actctttctc gtcgaagagc ttactcactt ccttttcaaa atgctcaaca gcctccgctg
     1081 gcaaatgacg tttcgcatct tgatacacaa gcttcatttc aactcgttgc atgagaaggc
     1141 gccgcatcag gtcgcgtttg tattgttcca aaacactggg cgggacctta tcttggtagg
     1201 aaagcagtat ctcgttgacg gcgccaagga tttcgccagc caagatgaca tctcccccta
     1261 ccttcgctaa cacggcgact tgttcacaaa cttcggagcc aaccgacgaa ccccgttcga
     1321 ttttggccgt ggcggcctgc acagcagggc cctcggacca ctttaaaggg gactcatctg
     1381 tgaccacaac ttcctgctga gatgccaagt tgacttgccg agattgatca tcatccgagg
     1441 ggacctgcca tgtctcaacc gaaggaaata cgggaggagg attggtgcta ggcgatagtc
     1501 tcgcttgcac ggagggcaaa ttggccccgt catccgtaac ttgactggcc agcgggacag
     1561 cctctgcctg catcggatcg atcaccgtca caagcctccc tgtgggcaag acgaactctg
     1621 gtcgggctgt tgcgggataa agctggtggg catctcctac cacctgat
//
LOCUS       DAOPTR010000959         1666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_56829_length_1666_cov_2.877095, whole genome shotgun sequence.
ACCESSION   DAOPTR010000959 DAOPTR010000000
VERSION     DAOPTR010000959.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56829_length_1666_cov_2.877095"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PKI05_14890"
     CDS             complement(<1..500)
                     /locus_tag="PKI05_14890"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA53538.1"
                     /translation="MGSSNTLNRSDVNPQCGGPNRMALPVVFDVRTVTETGGGPEKTI
                     LLGARWFEDRHWPVYCVYLHPPGDPGFEAIRQKAAELRLSYFLHLDDRGPLDGRLLRR
                     LLALCRQHRPVIWHGHDYKSDALGWLIHRFHPMKLVSTVHGWGVPGRRVWLYHWIHRL
                     CLRGFDR"
     gene            complement(513..>1666)
                     /locus_tag="PKI05_14895"
     CDS             complement(513..>1666)
                     /locus_tag="PKI05_14895"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOA53539.1"
                     /translation="ANGPLIVQPNAGIPKEVEHRRIYLCSPEYLAEYGKRYVNLGAAA
                     VGGCCGTTPDHIRALCDAVKPLVRARIKPVVVTTEPVALKEPVPFAERSRFAWRLAHK
                     QWVTSVELVPPRGFDLRDIIDKSKMLHRHGVDAINIPDGPRASSRISPLITADRIQRE
                     AMIEVILHFCCRDRNLIGMQADLLACAACDIRNILFVTGDPPKLGNYPHATGVFDTDS
                     IGMVAVQKRLNQGVDLGGQSIDPPTAAVIGVGLDPTAIDQKRELDRFQRKVEAGAEFA
                     ITQPVFDPDALLRFLDKVQHHGIPIIAGIWPLASLRNATFLKNEVPGVVIPDWVLDKM
                     ASVSSREDQLKMGIEIARYSVERIRDRVAGIQVSAPFGKAAIALEVIGA"
BASE COUNT          379 a          496 c          476 g          315 t
ORIGIN      
        1 cgatcaaagc cgcggagaca tagccgatga atccaatggt aaagccacac ccgacgaccg
       61 gggacgcccc aaccatggac cgtgctcacc agtttcatgg ggtgaaagcg atggatgagc
      121 cagcccaggg cgtccgactt gtaatcatgt ccatgccaga tcaccggtcg gtgttgccga
      181 caaagggcca aaagccgccg caagagccgc ccatcgaggg ggccccgatc atcgaggtgg
      241 agaaaatacg acagccgcaa ctcggcggct ttttggcgaa ttgcctcaaa tcccggatcg
      301 cctggcggat gaagatacac acaatacacc ggccaatgac gatcctcgaa ccagcgggca
      361 ccaaggagga tcgtcttctc cggaccgccg cctgtctccg ttacggtacg aacatcgaac
      421 accacgggca gggccatccg attgggtccg ccgcattgcg gatttacgtc agaccgatta
      481 agggtgttcg acgatcccat atcccttaca cttcacgccc cgattacctc taaggcaatg
      541 gcggctttgc caaatggggc actgacctgg atgcccgcca cacgatcgcg gatccgttcg
      601 actgagtacc gggcaatctc aattcccatc ttgagttggt cttcccggct ggataccgag
      661 gccatcttgt ccaaaaccca atcggggatt accacacccg gtacctcgtt cttgaggaac
      721 gttgcattgc ggagactggc tagcggccag ataccggcaa tgatgggtat accgtggtgc
      781 tggaccttat cgagaaaacg gaggagggca tcgggatcga acacgggctg cgtgatggcg
      841 aactcggccc cggcctctac cttccgctgg aaacgatcga gttcgcgttt ctggtcgatg
      901 gccgtggggt ccaaaccaac gccgatcacg gccgccgttg gcggatcaat ggactgccct
      961 cccaagtcca caccctgatt gagtcgcttt tgcaccgcca ccatgccaat cgaatccgta
     1021 tcaaagacgc ccgtggcgtg cggatagttt cccagtttgg ggggatcacc cgtcacaaag
     1081 aggatattgc gaatgtcgca ggcagcgcag gccaacagat cggcttgcat cccgatgaga
     1141 ttacggtcac ggcaacaaaa atggaggatg acctcgatca tggcctcccg ctgaatgcgg
     1201 tcagccgtga taaggggcga aatccgtgag ctagcccggg gaccatcggg aatgttgata
     1261 gcatccaccc cgtgtcggtg gagcatttta cttttgtcga taatgtcccg cagatcaaat
     1321 ccgcgcggcg gcaccaactc cacagaggtg acccactgct tgtgggcaag ccgccaggca
     1381 aaacgcgacc gctcggcgaa aggcaccggt tccttgagcg ccacgggttc agtcgttacg
     1441 accacgggct tgatccgagc acggacgagc ggtttgacgg catcacacaa cgcgcggatg
     1501 tggtcaggcg tggtgccgca acagccgccc accgcagcag ctccgagatt gacgtaacgt
     1561 tttccgtatt cagccaaata ttctggggag caaagataaa tccgccgatg ttccacttct
     1621 ttgggaattc ctgcgttggg ctgaacaatg agcggtccat tggcca
//
LOCUS       DAOPTR010000960         1655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_57383_length_1655_cov_2.858125, whole genome shotgun sequence.
ACCESSION   DAOPTR010000960 DAOPTR010000000
VERSION     DAOPTR010000960.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57383_length_1655_cov_2.858125"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..>1655
                     /locus_tag="PKI05_14900"
     CDS             212..>1655
                     /locus_tag="PKI05_14900"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53540.1"
                     /translation="MLIQQLMILLPCYSLEDFQVSRSDEEAEEILAGFCGLFHPALIH
                     QTQNMPRWERAYDPPIAPDQALIVIPECSEKCLPSTWLADLPPGQSVVVRRYRNLAGL
                     WEAIGHLTGKPLEVPQPQWIDDFIALGYAYLQVELMTRQLRYMSNLDELRFRDHTVKA
                     ATALFEGNTDQVKEHLQRSFDLLTESREYFYPVQTHLIDLTLTAGTTLGPGLQRELED
                     GKQVNVLTTGQLLVKMLHHYPKTLQSLKEALEAGRANVVGGEDEESASAFLPQTTLIR
                     HLVYGHKQWQEVLGSRPQIFGKRRFGLTPLMPGLLSSFRYEGVLHFALDDGKFPLPNQ
                     SKLRWEGLDEAVIDAVGRVPLDASRASTFLKLGETVGRALDLDHAATVIFAHWPGQVA
                     TWYKFLRRAAEYSPILGQFSLLAASFRNTQYVGSRLTYKADEYRPPYLAQAVAESQVD
                     PVGRWIRFVRVNLHAQVLSNLVVMRSLVNGR"
BASE COUNT          367 a          473 c          434 g          381 t
ORIGIN      
        1 atacagccgc cacctttgag ggtaacagaa acgacgattt cgctccatag aagttttttt
       61 gccctcgcgg agggttattt gccgttttgt acacactgcc ggatcctccg tgccacgcgt
      121 tcacagcggc agccaaagtc cagacctgct atactagaga atcttacgag gattgttgcc
      181 caaatcgcct ttcaaggagg caaataagca gatgttgatt caacaactca tgattcttct
      241 tccgtgctac agccttgaag actttcaggt cagtcgcagt gatgaagaag cggaagagat
      301 tctcgcgggt ttttgtgggc tatttcatcc ggctcttata caccaaaccc aaaacatgcc
      361 gcgatgggag cgagcctatg atccccccat cgcccctgat caggccctga ttgtcatccc
      421 cgagtgttcg gaaaaatgcc ttcccagcac ttggttggcg gatttgccgc ctggccaatc
      481 agttgtcgtc cgacgctacc gcaatcttgc cggtctttgg gaggcaatcg gccatctgac
      541 gggcaaaccc ctggaagtcc cgcaacccca atggatcgac gactttatcg ccctgggata
      601 cgcctatctt caagtcgaat taatgacccg tcagcttcgt tacatgagca accttgacga
      661 attgcgattt cgcgatcaca cagtcaaggc ggcgacagca ctgttcgaag gcaacaccga
      721 ccaagtgaaa gagcatctcc aaaggagctt cgatctgttg acagaatcaa gggagtactt
      781 ctaccccgtt caaacccacc tgatcgacct gacgctgacc gcaggtacca cacttggacc
      841 agggttacag cgcgaactgg aggacggcaa acaggtcaat gtcctgacca caggccaact
      901 tctcgtaaaa atgttgcacc attatccgaa gaccttgcaa tcgctgaaag aagccttaga
      961 agctgggcgt gcgaacgtcg tggggggcga ggacgaagaa tccgcaagtg ccttcctacc
     1021 ccaaacgacg ttgatccgac acctggtgta tggtcacaaa caatggcagg aagtcctagg
     1081 aagtcggccc cagatttttg gaaaacggcg ttttggactg accccgctca tgccgggact
     1141 gttgagcagc tttcgctacg agggggttct tcattttgcg ttagatgacg gcaagtttcc
     1201 gctccccaat cagagcaaac tccgctggga aggcttggac gaagccgtga ttgatgccgt
     1261 ggggcgggtt cccctggacg cttcccgggc atcgacgttc cttaagttgg gcgagactgt
     1321 cggtcgagcc ctcgatttgg atcacgcggc cacggtgatc ttcgcccact ggcccggcca
     1381 ggttgcgaca tggtacaagt tcctccgccg ggccgccgaa tattcgccga ttcttggtca
     1441 gttttccctt ctggcagctt cctttcggaa cacccagtac gttgggtcac ggcttacgta
     1501 taaggccgat gagtaccgtc ctccctacct tgcccaagcc gtcgccgagt cacaggtcga
     1561 tcctgtcggc cgatggatac gctttgtgcg ggtgaacctc cacgcgcagg tcttgtccaa
     1621 tcttgtcgtg atgcggtcac ttgttaatgg ccgct
//
LOCUS       DAOPTR010000961         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_57573_length_1651_cov_2.940476, whole genome shotgun sequence.
ACCESSION   DAOPTR010000961 DAOPTR010000000
VERSION     DAOPTR010000961.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57573_length_1651_cov_2.940476"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PKI05_14905"
     CDS             complement(<1..664)
                     /locus_tag="PKI05_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53541.1"
                     /translation="MRPGCAKEAGRTSGTPSPKQRRQGDSSRRNLRSASATGSADSFY
                     HRQWQDHWWLDAETWASGGTYQNATYDDYNYQYRWTKTWQGQGDGAVSSSASNHVTGL
                     ENGSESGWWTHTVPEGSGSGTGSSSNTSWGSPWSHSYDETITYPGFYDYRYYGRDYGG
                     GSGEEDHIAYVGDVFHESPGSDTGFFPFACSGEDPFFAQAEGGGHYAILVDAVANSTG
                     EQE"
     gene            complement(793..>1651)
                     /locus_tag="PKI05_14910"
     CDS             complement(793..>1651)
                     /locus_tag="PKI05_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53542.1"
                     /translation="IQADDAVIGEPPRCEACGHPVGMRAWLPPVRVNFEVCRDGYCDF
                     VFPPVNDILVSSRFKQIYESEGLTGLSGFDPVEIVKIKPKRRAKEPPPAYFRVFVARS
                     QAVVDPEASGFDPPADKPVCPVCRIGSNRKRWKKLVIQPGTWSGEDVFFPRGCTGDII
                     VSERFKMVCEAHGVTNALLIPVEEYGYDFYPHETKDWDIRKYEETLAVLRTMNRAGRL
                     DDYVEAMEEMRKYVLADPKFDWLEELRERFEGKIDPIGDAAHDAYYNLIAPWYAKYVR
                     KPKGPSRRT"
BASE COUNT          307 a          546 c          468 g          330 t
ORIGIN      
        1 cttcttgttc tccggtcgaa ttcgcaaccg cgtcgacgag gatggcgtag tgcccgccgc
       61 cctccgcctg tgcaaagaat gggtcctcgc cgctgcaggc gaacgggaag aaaccggtgt
      121 cgctgccggg gctttcatgg aaaacgtcgc ccacgtaggc gatgtggtcc tcctccccgg
      181 agccgccgcc gtagtcccgg ccgtagtagc gatagtcgta gaagccggga taggtaatgg
      241 tctcgtcgta agagtggctc cacgggctac cccaactggt gttggacgaa gagcccgtgc
      301 ccgagccgct cccctccggc accgtgtgcg tccaccagcc gctctcggag ccgttctcca
      361 ggccggtcac gtggttgctc gccgaactgc tcaccgcacc gtccccctgg ccctgccagg
      421 tcttggtcca ccggtactgg taattgtagt cgtcgtaggt ggcgttctgg taggtcccgc
      481 cgctggccca ggtctcggca tcgagccacc agtggtcctg ccactggcgg tggtagaagg
      541 agtcggccga gcccgtggcc gaggccgagc gcagattcct ccgagaggaa tcgccctgac
      601 gccgttgttt cggggacggt gtccctgagg tgcggccggc ttccttggca cagcctggcc
      661 gcacacccga aatgcgggct tggactcatg ccggaccccg catctcgctt gctgccctct
      721 gcagctgcca cctgtcggct ggcccaaagg cagacgggag taggctactc catcatctca
      781 acgagcactt agtcacgtgc gccggcttgg ccccttgggc tttctcacgt acttcgcgta
      841 ccagggagcg attagattat aataggcgtc atgtgcggcg tctcctatgg ggtcgatttt
      901 tccctcaaag cgctcccgca gctcttcaag ccaatcaaac ttggggtctg ccagcacgta
      961 cttcctcatc tcctccatcg cctccacata atcatccaat cgcccagccc tgttcatggt
     1021 ccgcagcacg gccaacgtct cttcgtactt ccggatgtcc cagtccttgg tctcatgagg
     1081 ataaaagtcg taaccatact cttccactgg gatcaagagc gcgttagtca ccccatgggc
     1141 ctcacagacc atcttgaacc gttcggagac aatgatgtca cccgtgcagc cacgcgggaa
     1201 aaagacatcc tctcccgacc acgttcctgg ctggatgacc agtttcttcc aacgctttcg
     1261 attagaccct atccggcata ccgggcagac cggcttgtcc gcagggggat cgaaaccgga
     1321 tgcttcagga tccaccacgg cttggctccg cgccacgaag acgcgaaaat acgcaggagg
     1381 cggttccttg gctcgccgct tgggtttgat cttcacgatc tccacagggt caaaaccgga
     1441 aagcccggtt agcccttcgg actcatagat ctgcttgaac cgagaggaga cgagaatgtc
     1501 gttcaccgga ggaaatacaa agtcacagta cccgtcccgg caaacctcga agttgacccg
     1561 caccggcggc aaccaagcgc gcattcccac gggatggccg caagcctcac aacgcggcgg
     1621 ctcgccgatc accgcgtcgt cggcctgaat g
//
LOCUS       DAOPTR010000962         1650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_57629_length_1650_cov_2.634483, whole genome shotgun sequence.
ACCESSION   DAOPTR010000962 DAOPTR010000000
VERSION     DAOPTR010000962.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57629_length_1650_cov_2.634483"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PKI05_14915"
     CDS             complement(<1..885)
                     /locus_tag="PKI05_14915"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOA53543.1"
                     /translation="MPLRVLFINQHFAVPHQGAGTFPFTVMSELVRRGHEVTVLTTDQ
                     HSSHGRSYVTEENGICVHWACIPYANRMRYWRRIVAFFEFTAWCLAKIRRLAPFDVVY
                     ASSTPLTVALPGVYASRRWAVPFVFEVRDLWPEVPIAMGALRNPVSIAAARWLERFAY
                     RNAAFVIARSPMMADGVVETGYPRERVRVVPNACDFRRFDVPAEAGRSFRQENQWLAD
                     RPLVLYAGTFGAVNDCSYIVRLAAEVEPLLPEARFLLVGQGREENLIRNLAQELGVLD
                     RTLFIHPPVPKSEVPRLFS"
     gene            complement(1179..>1650)
                     /locus_tag="PKI05_14920"
     CDS             complement(1179..>1650)
                     /locus_tag="PKI05_14920"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOA53544.1"
                     /translation="WAIEDPVEEVRLHALEALAPLKSGEIVSYFVGRLRDKDNAIVNR
                     AAQALQRMGDPSAIPALIDALVTRHKIFIPGTRPPTGMAAGFSSSSTGKSGMSFGAGS
                     GGDTPKVVERPFSNPAVLEALVELSGGQNFGYDVRLWKHWYATQKQAEATKAMQ"
BASE COUNT          381 a          491 c          489 g          289 t
ORIGIN      
        1 ggaaaacagc cgcgggacct cacttttggg aaccggcgga tggataaaaa gggttcggtc
       61 aagtacgcct agctcttggg caaggttgcg aatgaggttc tcctcccgac cttgccccac
      121 gagcaggaaa cgcgcctccg gtaggagggg ttcgacctcg gccgccagcc gcacgatgta
      181 ggagcaatcg ttgacggccc caaaagtgcc ggcataaagc accaacgggc ggtcggccaa
      241 ccactggttc tcttgacgga acgaacgacc cgcctcggca gggacgtcaa aacgacgaaa
      301 atcgcaggcg ttcggtacga cgcggacccg ctcgcgcggg taacctgttt ccacaacacc
      361 gtccgccatc atgggcgaac gcgcgatgac gaaggcggcg tttcgataag cgaaccgctc
      421 gagccagcga gcggcggcga tgctcaccgg attgcgtaac gcgcccatcg caatgggaac
      481 ctcgggccaa aggtcgcgga cctcaaaaac gaaggggaca gcccatcgtc gtgaggcgta
      541 aaccccaggc agcgccacag ttaggggcgt actggacgca tagacgacat cgaaaggtgc
      601 taatcgcctt atcttcgcca ggcaccaggc ggtgaactcg aaaaaggcca caatccgccg
      661 ccaatatctc atgcgattcg catacggaat gcacgcccag tgaacgcaga taccgttctc
      721 ttcggtgaca tagctccggc catgggacga atgctgatcc gttgttaata ccgtgacctc
      781 atgtccgcgg cgaacaagtt ctgacatcac cgtaaaggga aaagtgcccg ccccctggtg
      841 cggcacggcg aaatgctggt ttataaataa cacgcgtaaa ggcattgctt gactccgacg
      901 accttgatcg tccggaagac acacctggcc cacaatcgtt ggaagacaaa aatgccatcc
      961 cgccgcgcgt ggtagccagg gtcttgcgca ggcggtgatc atatcagagc cagcggaaac
     1021 ggggcaaccc catttctcaa tggggtaagc gcgcaggtgg gtagcgaaac tgtgggagaa
     1081 ggttaagcga caaacagctc catcggccgg cccgcgtcgg cacttgcgtc accgcgaaaa
     1141 ggcacgcctt gtcacagttt ctgtgatacc gccgattttt actgcatggc cttggttgcc
     1201 tccgcctgct tctgcgtggc gtaccaatgc ttccacaggc ggacgtcata cccgaagttc
     1261 tggccaccgc tcagctcgac gagggcttct aacacggcag ggttcgaaaa aggtcgttcc
     1321 acaactttag gagtatcgcc gccggaacca gctccaaacg acattcccga cttgcccgtg
     1381 ctggaactgc tgaatcccgc tgccatgccg gtggggggac gagtgccggg gatgaaaatc
     1441 ttgtgtcggg tgacgagggc atcgatcaac gcgggaatgg ccgacgggtc ccccatgcgc
     1501 tggagagcct gtgcggcacg gttaacgatg gcgttgtcct tgtcgcggag tcgcccaaca
     1561 aaatagctca caatctctcc tgacttgaga ggtgcaaggg cctccaaggc atggagccga
     1621 acctcctcga cgggatcctc aatggcccaa
//
LOCUS       DAOPTR010000963         1649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_57663_length_1649_cov_2.757215, whole genome shotgun sequence.
ACCESSION   DAOPTR010000963 DAOPTR010000000
VERSION     DAOPTR010000963.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57663_length_1649_cov_2.757215"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..1434
                     /gene="nuoF"
                     /locus_tag="PKI05_14925"
     CDS             46..1434
                     /gene="nuoF"
                     /locus_tag="PKI05_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOA53545.1"
                     /translation="MMESFEPVLLANIQKPDSHTLRAYEASGGYATLRRVLKELSPAE
                     VVEEVRISGLRGRGGAGFPTGLKWSFLPADHPGPIYLCVNADESEPGTFCNRILLELD
                     PHQVLEGTIISAYATRATTAYVYLRYEYPLAYRRLSEAIREAYQAGYLGKNILGTGLD
                     LDMYIHRGAGAYICGEETGLIESIEGKRAWPRTKPPFPAVEGLFRKPTVVNNVETLAC
                     VRHIMERGAEWFRSIGVPPDPNDPRDPGSFGPKLFCVSGHVNRPGCFEAPLGIPCRRL
                     IEEYAGGVRGGRRAKAVVPGGLSTGLLSADELDTPMDFAGPIKAGCLGLGTGGVIVLD
                     ETYPIIDFLHNSCRFFAHESCGQCTPCREGTAWALQIVGRIKAGRGRLVDLDLLLEIA
                     DNIGIIPGTTICGLADGAAWPIKTAIRKFRGELEDYIKRTNPKGWRSTRPVPAFSLEK
                     TDQANSMRTPVA"
     gene            1463..>1649
                     /locus_tag="PKI05_14930"
     CDS             1463..>1649
                     /locus_tag="PKI05_14930"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOA53546.1"
                     /translation="MGIVIVDGKEVEIGAHERLNGIQVAARAGIEIPHYCWHPGLSVV
                     ASCRMCLVEVGTRDPKTG"
BASE COUNT          349 a          412 c          518 g          370 t
ORIGIN      
        1 atcagtcctc gatgaactat cgaagcaagg tataaacgga catggatgat ggaatcgttt
       61 gaacccgtcc ttttggcaaa tattcaaaag ccagacagcc acaccttgcg agcttacgag
      121 gcgagcggtg gctacgcgac gctccgccgt gtcctcaaag aattgtcacc cgcagaggtg
      181 gttgaggaag tccggattag tggtctccgc ggtcgtggtg gagcgggctt cccgacaggt
      241 ttgaagtggt cttttcttcc agccgatcac ccggggccaa tttacctttg cgtgaacgct
      301 gatgaaagcg agccaggaac attttgcaat cgcattcttt tggaattgga tcctcaccag
      361 gtcctggaag ggacgatcat tagcgcgtac gcaacccggg cgacaacagc gtacgtgtat
      421 ttgagatatg aatatccgct agcttaccgg cgtttaagcg aggcgattcg ggaagcctat
      481 caggcgggtt atctggggaa gaacatcttg gggacgggct tggatttgga catgtatatc
      541 caccgcggcg ccggtgccta tatttgcggc gaagagaccg gtttgatcga aagcatcgag
      601 ggcaaacggg cctggccgcg gacaaagcct cccttcccgg cggtggaagg attattccga
      661 aaacccaccg tggtaaataa tgtggagacc ctggcctgtg ttcggcacat catggagaga
      721 ggcgcagagt ggtttcgctc gatcggtgtg ccgcctgacc cgaatgatcc gcgggatcca
      781 ggaagtttcg gccctaagtt gttttgcgtc agtggccatg tcaatcggcc aggttgtttt
      841 gaagcgcctt tggggattcc atgtcgccgg ctgatcgaag aatatgccgg gggtgtgagg
      901 ggtggacggc gggccaaggc agtggtgccg ggcggactga gcaccggtct tctttcggcg
      961 gatgagcttg atacgcctat ggattttgct ggccccatca aagccggctg tctgggcttg
     1021 ggcaccggcg gcgtcattgt cctcgacgag acgtatccca tcattgactt cttgcataat
     1081 agttgtcgct tttttgccca cgaaagttgt ggacaatgca cgccttgccg ggagggaacg
     1141 gcttgggcct tacagattgt gggacgcatc aaggcaggac gcggtcggct ggttgacctc
     1201 gaccttcttt tggaaattgc cgacaacatt ggcattattc cgggcaccac aatttgtggt
     1261 cttgccgatg gagcggcgtg gccaatcaag acggccatcc gcaagttccg cggtgaattg
     1321 gaggactaca tcaaacggac gaaccccaag ggatggcgtt ccacgcgccc ggttccggcc
     1381 ttttctctcg aaaagacgga ccaagcaaat tccatgcgaa cacccgttgc ctgattggga
     1441 ggaagatgag ggactgaatt ccatgggcat tgtgatcgtt gacggcaaag aggtggagat
     1501 cggcgcgcac gagcggctga acggcattca agtggccgcg cgggcgggca ttgaaatccc
     1561 tcattactgc tggcatccgg ggttatctgt ggtcgccagt tgtcggatgt gccttgtcga
     1621 ggtgggaacg cgcgatccaa agaccggac
//
LOCUS       DAOPTR010000964         1647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_57740_length_1647_cov_4.152010, whole genome shotgun sequence.
ACCESSION   DAOPTR010000964 DAOPTR010000000
VERSION     DAOPTR010000964.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57740_length_1647_cov_4.152010"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="PKI05_14935"
     CDS             <1..993
                     /locus_tag="PKI05_14935"
                     /inference="COORDINATES: protein motif:HMM:NF014500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOA53547.1"
                     /translation="RMTKSHYSLSILAIGAGAALTHTLVPPTPGPLAVAGTLGVEVGH
                     MILVGILVGVPAAVLVYPIIRLLDRSMPLPVRPYVGEVETPPAQEQDLPPFWLSAMPV
                     ILPVLLISANTIIKVLVKSYPSWQTLANVTGVIGDPNAALLLAAVVAMGTVAWKRHLS
                     LEALGRLTEEALMSAGVIILITSAGGAFGAMLRQTGINEEIKVFVGSQSSMGGVVLLA
                     MSFLVAAVMKTAQGSSTVAMLTTAAIMTALFDTPESLVQHLGCHPVYMALAIGAGSLV
                     FCWMNDSGFWVVARMSVWSELEALKLWTVTAACAGLAAFLVTVVFALVLPNALG"
     gene            complement(1057..>1647)
                     /locus_tag="PKI05_14940"
     CDS             complement(1057..>1647)
                     /locus_tag="PKI05_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HOA53548.1"
                     /translation="AHVPFYIVQGELDGTSMPDNARDLDRYLNRAFDVTVVEYMGRGR
                     DPFFDEILRLFEWMSYKQRNPAPTDFTARSMRAFDNFFWFIELADMPPAVITPPVNWP
                     PRNPTPLVVRGRIPSPNTLLLQVGSARSIIGLGPEFVDFDKRLAVTINAQRVDTRDVQ
                     PDIEVLLEDVRRRGDRQHPFWAKIETATGRVSGKRP"
BASE COUNT          328 a          420 c          506 g          393 t
ORIGIN      
        1 cggatgacga aatcacatta ttcgttgtcg attctcgcca ttggcgcggg ggccgccctg
       61 acgcacacgt tggttcctcc gacgccgggg cccctggccg ttgccgggac gttgggtgta
      121 gaggtgggcc acatgattct ggtcgggatc ttagttgggg tgcctgccgc cgttttggtg
      181 tatccaatta ttcgccttct cgatcggtct atgccgcttc ctgtccggcc ctatgtgggc
      241 gaggtggaaa cgccaccagc gcaggagcag gacctgccgc cgttttggct atctgcgatg
      301 cctgtgatcc ttccggtttt gctgatatcc gccaacacga tcatcaaggt gctggttaaa
      361 tcttatccga gttggcagac tcttgcgaat gtcacgggcg tgatcgggga cccgaatgcg
      421 gcacttcttt tggcggccgt ggttgctatg gggacggtag cgtggaaacg ccatttgtcg
      481 ttggaagcat tgggacgatt aaccgaagag gcattgatga gcgccggagt cattattctc
      541 atcacttcgg caggtggcgc tttcggggcc atgctaaggc aaactggcat caatgaggaa
      601 atcaaagtgt ttgtgggatc gcaatcgagc atggggggtg tcgttttgct ggcgatgagt
      661 ttcctggtgg cggccgtgat gaagacggct cagggatcaa gcactgtggc catgttgaca
      721 accgcagcca tcatgacggc actattcgac acaccagaat cgttggttca gcatttggga
      781 tgccacccgg tctatatggc attggccatc ggcgcagggt cgctcgtctt ttgttggatg
      841 aacgatagtg gtttttgggt cgttgctcgg atgagcgttt ggtcggagct tgaggcgctc
      901 aaattgtgga cggttacggc agcctgcgct ggtttggctg catttttggt aacggtggtc
      961 tttgccttgg tgctccccaa tgcattgggc tgatgttcgc gcaaaccacg tcaaaaaagt
     1021 cggttaattg tgatctcgtt tcgacggccg gatagctcat ggccgtttgc ccgagacacg
     1081 gcctgtcgca gtctcaatct tcgcccaaaa aggatgttgc cggtcgccgc ggcgacgcac
     1141 gtcttccagc aggacctcaa tgtctggctg aacgtcccgc gtgtccactc gttgagcatt
     1201 aatcgtgaca gcgagacgct tgtcgaaatc gacaaactcc ggccccaagc caatgatgct
     1261 tcgggctgag cccacttgga gtaagagggt gtttggcgag gggattcgcc cgcggacaac
     1321 aagcggtgtc ggattccgtg gcggccagtt taccggcggg gtgatgacag ccggcggcat
     1381 atcggcaagc tcgataaacc agaagaaatt gtcgaacgcc ctcatcgaac gagccgtaaa
     1441 atccgtcgga gccggattcc tttgcttgta agacatccac tcgaagagac gaagaatctc
     1501 gtcgaagaaa ggatcgcgac ctcggcccat atattccacg acggtgacat caaaggctcg
     1561 attgagataa cgatcgagat cgcgggcatt gtcgggcatc gatgttccgt ccaactcgcc
     1621 ctgcacaatg taaaagggaa catgggc
//
LOCUS       DAOPTR010000965         1639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_58129_length_1639_cov_3.122475, whole genome shotgun sequence.
ACCESSION   DAOPTR010000965 DAOPTR010000000
VERSION     DAOPTR010000965.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58129_length_1639_cov_3.122475"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..993
                     /locus_tag="PKI05_14945"
     CDS             277..993
                     /locus_tag="PKI05_14945"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOA53549.1"
                     /translation="MSEVKAVSIKPRDKDLSGPPFVNVWWYEFSRVWVRLAAWVFFRI
                     RYSGVENIPRTGPVLLVSNHQSHLDPPLIGAGVPRTVSYLARKTLFTNKFFSRLITSY
                     GAIPLDLENPLSGLKEALRRLRCGDAVLIFPEGTRSPDGEIHPFRKGFRVLAVRSQAI
                     IVPLAIEGAYQSWPRSRKFPRPGRIRVHFGRAIGPQDYENLDEEAFIRMVEEKVRQCL
                     ATIRSESGNVRRNDGANVSP"
     gene            complement(1104..>1639)
                     /locus_tag="PKI05_14950"
     CDS             complement(1104..>1639)
                     /locus_tag="PKI05_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53550.1"
                     /translation="GIMEPNPAKQRNILATNTTRGSAVEIAHDGQRLREIIVPGRTIA
                     WLVCADLDGDGRSELCGLDPKDFGQVEAFAFNEEGEELWSYKLPKGMTEYPVEQITHG
                     RLLPNQDTQQWVFVGADGSLHIVDINGQLVDQFNVGELITGVAVARFSGKPALIVATP
                     QRVLAWSVEANETKATQ"
BASE COUNT          345 a          429 c          453 g          412 t
ORIGIN      
        1 taaatagccc agaatatata ttcttgcatt cgcagggtgt tcggggccct ccttattttt
       61 tcaaggaggg tgaccaagtg ccgcatgtcc gcgagagttc gcacatgcgc actcggatag
      121 agggcagggc ttgctcctgt tgtctcaaac ggaccctttc gccggtatct tgttgggttt
      181 acactcgaca aaaaaccgta catacacaag aggctggtta ctgctgccta aaaagcagtg
      241 ctcgtgagtg atgacacctg gagggtgata acagttgtgt ccgaagtcaa ggctgtatcg
      301 ataaaacctc gagataagga tctttctggt ccgccatttg ttaacgtatg gtggtacgaa
      361 ttcagccggg tttgggttag gctggcggct tgggtgtttt tcaggattcg ttattcaggt
      421 gtcgagaata ttccccgcac tgggcccgtg ctgctggtct ccaatcatca gagccacctc
      481 gacccaccac tgattggagc aggtgttccg cggacggtga gttatcttgc ccgtaagacg
      541 ctcttcacca acaagttctt ttcgcggctg attacctctt acggtgccat ccccctcgat
      601 ctggagaatc ccctgagcgg cttgaaagag gccctgcgga ggcttcggtg tggggacgcc
      661 gtgttaattt ttccggaggg gacgcgttct cccgacgggg aaatccatcc ctttcggaaa
      721 ggatttcgcg tcctggcggt tcgatctcag gccataatcg tgccactggc gattgagggg
      781 gcctatcaat cgtggccaag gtcgcggaag tttcctcgcc ctggtcgcat acgggttcac
      841 tttggccggg caattggtcc ccaagactac gaaaacttag atgaagaagc ctttatccgc
      901 atggttgaag aaaaagttcg gcaatgccta gcgacaattc gaagtgaatc gggcaacgtg
      961 cgccggaacg atggtgcgaa cgtctcacct taaggcgcag ttttcgtgaa acaaatcgca
     1021 cctggtcaca aaatggcgat tgccgcaacc tcgtgacaac acgtgtccga gctttagcac
     1081 attcgagtat cgccttcctc ggatcactgg gtagcttttg tttcgtttgc ctcgactgac
     1141 catgcgagga ctctttgggg cgttgctacg atgagggccg gcttccctga gaatcgcgcg
     1201 accgccacgc ccgtgatgag ttcacccacg ttaaattgat cgaccaattg tccgttgatg
     1261 tccacaatgt gaagtgaacc atcggcacca acaaagaccc attgttgggt atcctgattt
     1321 ggcaggaggc gtccatgggt gatttgctcg acggggtatt cggtcatacc tttggggagt
     1381 ttgtaactcc ataattcttc cccttcctcg ttaaaggcga acgcctcaac ttggccaaaa
     1441 tcctttggat cgaggccgca caattcagat cgaccatcgc cgtccaaatc ggcgcagacg
     1501 agccaggcaa tggttcgccc cgggacgatg atttcccgca gacgctgacc atcgtgggca
     1561 atttccacgg cgctgccgcg ggtcgtattg gtggctaaga tgttgcgttg tttcgccggg
     1621 ttcggctcca tgattccta
//
LOCUS       DAOPTR010000966         1636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_58296_length_1636_cov_2.821632, whole genome shotgun sequence.
ACCESSION   DAOPTR010000966 DAOPTR010000000
VERSION     DAOPTR010000966.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58296_length_1636_cov_2.821632"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="PKI05_14955"
     CDS             <1..731
                     /locus_tag="PKI05_14955"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOA53551.1"
                     /translation="ITTTIRGLKHHGVAAGAPPCLPGRPIPKEYFEENLAWLEAGAAN
                     LLHHINTVKKSGVHPVVAINVFPTDTQEEIRLLKRIAEQSGARVAVSNHWRYGGDGAL
                     ELADAVLDACEEPVHFRYLYNMDTPLSERIERIAREVYGADGVEYSREAEAKLKEFES
                     NPKYHDYATMMVKTHLSLTHDPTVKGAPKGWTLPIRDILQYGGAKFLCPMAGEISLMP
                     GTGSDPAFRRIDVDVKTGRVTGLF"
     gene            complement(838..>1636)
                     /locus_tag="PKI05_14960"
     CDS             complement(838..>1636)
                     /locus_tag="PKI05_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOA53552.1"
                     /translation="KASPKKSSEPLPAPRRERLPDTRRSITHKFSINGHEGYITVGLY
                     PDGRPGELFITMSKEGSTIGGLMDCFGTAVSMSLQYGVPLEVYVNKFSYTRFDPMGFT
                     KNPEIRTATSIVDYIFRWLGMMFLPGYREKERNMRKQLEKVAHSEVTQPQTGKPPVSG
                     TEQGVSSEDGKGVQGNPAASDPWLVVGGPLPASLKGIAQSLSISGNGQHQVATDQTRG
                     EESLDMASEVFPVIRDDGPPCDVCGAITRRCGSCFVCLNCGRSLGCS"
BASE COUNT          379 a          475 c          439 g          343 t
ORIGIN      
        1 taatcacgac aacaattcgc gggctcaaac accacggtgt tgccgccggg gcaccgccgt
       61 gcttaccagg acgtcccatt ccgaaagagt actttgagga aaacctcgcc tggcttgagg
      121 ctggggccgc caatctgttg catcacatca atacggtcaa gaaatcgggt gtccatccgg
      181 ttgtcgccat caacgtattt cccaccgaca cccaagagga gatccgtctc ctcaagcgta
      241 ttgcagagca gagtggggct agagtcgcgg tctccaatca ctggcgttac ggtggggatg
      301 gggccttgga gttggccgac gccgtgctcg acgcctgcga agaacctgtc cattttcgtt
      361 acctttacaa catggataca ccactcagtg aacggatcga acgaattgcc cgggaggttt
      421 acggggcgga tggcgtggag tactctcggg aggcagaggc caagctaaag gaattcgaat
      481 ccaatccgaa ataccacgat tacgcgacaa tgatggtgaa aacccatctc agcctcacac
      541 acgatcccac ggtgaaaggc gctcctaagg ggtggaccct gcccatccgt gacatcctgc
      601 aatacggggg cgcaaagttc ctctgtccca tggcgggaga aatctcgctc atgccaggaa
      661 ccggttccga tccggcgttt cggcgtattg atgtcgatgt caagacaggc cgcgtgaccg
      721 ggcttttctg agttggttga agaaccgcca aagaaggaat taaaaaccga cggcaaacgc
      781 cacaatgtgg gcttgccgtc ggtcgccagc gatgtcgaac agtcctaagc cgtcgtatca
      841 gctacaccca agactgcgtc cgcaattgag gcaaacaaag caactgccac agcgccgggt
      901 gatggctccg caaacgtcac atggaggacc atcgtcacga atgaccggga agacctcgct
      961 cgccatatcc aagctctcct ctcctctggt ttgatcggtc gccacttgat gctgtccgtt
     1021 cccggagatc gacaacgatt gagcaatccc tttcaagctg gcaggcaacg gacctcccac
     1081 aactaaccaa ggatcagaag cagccggatt tccttgcacc cctttcccat cctcagacga
     1141 cacgccctgt tccgttccgg acacaggcgg cttaccggtc tgcggctggg tcacctcgga
     1201 gtgagccacc ttttccaatt gcttgcgcat gttccgctct ttttcacgat agccgggcaa
     1261 gaacatcatc cccagccacc ggaagatgta atcaacgata cttgtggccg tgcgaatctc
     1321 gggatttttg gtgaatccca taggatcaaa tcgggtgtag gaaaatttgt tcacatacac
     1381 ttccagcgga acaccatact gaagactcat ggagactgcc gttccaaagc agtccatcaa
     1441 gcccccaatg gtgctgcctt ctttcgacat cgtgatgaac aattcaccgg gccggccatc
     1501 gggataaagc cccaccgtga tgtaaccctc gtgtccattg atggaaaact tgtgcgtgat
     1561 ggaccgccgg gtgtcgggga gtcgttcccg ccgtggagcc gggagtggtt cagaggactt
     1621 tttcgggctg gccttt
//
LOCUS       DAOPTR010000967         1636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_58307_length_1636_cov_2.299178, whole genome shotgun sequence.
ACCESSION   DAOPTR010000967 DAOPTR010000000
VERSION     DAOPTR010000967.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58307_length_1636_cov_2.299178"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PKI05_14965"
     CDS             <1..358
                     /locus_tag="PKI05_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53553.1"
                     /translation="TPLCELEILETGQLVVAGQRALLAGAAEPITTGIVNAMVLGGAT
                     LRQAVAMATITPCEVLKIEPPQSLPGTPANLVVFEIEQDNEQFASSDKPDLLPARKQK
                     LAVRQVHFHGEVKTFG"
     gene            complement(401..745)
                     /locus_tag="PKI05_14970"
     CDS             complement(401..745)
                     /locus_tag="PKI05_14970"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOA53554.1"
                     /translation="MTRRVREAAGYVELGLFDRALECLEIEDLGPWEGPVAMFKGQIL
                     AAQGRFHDAAAAFERAAQLFPPPHDRLAWLTLSQCLRRAGDTTRAIQVLGRARGAFPR
                     QLFLPDAGAGSE"
     gene            complement(1029..>1636)
                     /locus_tag="PKI05_14975"
     CDS             complement(1029..>1636)
                     /locus_tag="PKI05_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53555.1"
                     /translation="IRLDVDEEAKRRGAFLLAIAVVSDDSNFVRNQPGLGEILQQIES
                     DDERKLRLSRIGIATIFGRHDWAQHFCVSAGLVCLLGAGPTRSLGLAKEWRDSQGDSG
                     WSFADLGADFAGIRFGEAILKGEIPLEDVRAGFQIADFVPPLADYPEDIPFTRFVATY
                     GGLLDSQLGRSWRRSTKLFRPCRATSEQIDDSFFEPVHLVV"
BASE COUNT          398 a          493 c          424 g          321 t
ORIGIN      
        1 caccccgctt tgtgaactag aaatcttgga gacgggtcaa ctggtggttg ccggtcagcg
       61 ggcactgctt gccggggctg ccgagcccat caccaccgga atcgtcaatg ccatggtcct
      121 aggcggggca acgttacgac aagcggtggc catggcgacc atcactccct gcgaggtgct
      181 caaaatcgag ccgccccaat ctctcccggg cacgcccgct aatctggtgg tctttgagat
      241 cgaacaggac aatgaacagt tcgccagctc ggataagcct gaccttcttc cggcgcgcaa
      301 acaaaaactc gccgttcgac aggtacattt ccacggcgag gttaagactt ttggttagcg
      361 gctatcagtt agcggctatc aacggctcgg agaatttgat tcactcgctg ccagcgcctg
      421 catccgggag gaaaagctgt cgcggaaatg ccccgcgagc acgcccaagg acctggatag
      481 ctcgggtcgt gtcaccggcc cttcggagac attgacttaa ggtcagccaa gccagacgat
      541 cgtggggtgg ggggaatagc tgcgctgccc gctcaaatgc cgccgccgca tcgtggaacc
      601 gcccctgggc agccaagatc tgccctttga acatggcaac aggcccttcc catggcccca
      661 ggtcttcaat ctcgaggcat tccagggcac ggtcaaacag acccagttcc acgtacccag
      721 cggcttcgcg aaccctacgt gtcactaaga tggctcgacg ttgctcggga tgcatttaac
      781 aacctccgta cccaatcata ggggggagta ctgcgatgca acaccggatc aagccttaat
      841 ctgaacaaag taaaggagca acctacatgc caaaaatcac caaaaacaca taatacgtaa
      901 gtaatttcca gataatgaca tacgtctgcg taaagtcttt gaccaagttc cattctcccc
      961 attttgacac actcggcgga cagagacgtg ccaatctgac acacactcgg cccacatcat
     1021 aagggggatc acactaccag atggacgggt tcaaagaacg agtcatcgat ttgctcgctg
     1081 gtagcccggc aaggcctgaa tagctttgtc gatctccgcc aggatcgtcc gagttggctg
     1141 tccaatagac caccgtaagt agctacgaat ctcgtgaacg ggatatcctc cggatagtcc
     1201 gctagagggg gaacaaaatc cgcaatctga aagccggcac ggacgtcttc gaggggaatt
     1261 tcccctttca agatggcctc accaaaacgg atgcctgcaa aatcggcccc aagatcggcg
     1321 aagctccagc ctgagtctcc ctgggaatct cgccactcct tggccaaacc caggctcctc
     1381 gtcggccccg ccccgagaag gcagactagc cctgccgaga cacaaaaatg ttgtgcccag
     1441 tcgtggcgac caaaaatcgt ggcaatcccg attcgcgata gtcgcagctt tcgttcgtca
     1501 tcggactcga tttgctgaag gatttcccct aagcccggtt gattccggac aaaattggag
     1561 tcgtcactga ccacggcaat cgccaagaga aaagcacccc gacgtttcgc ttcttcatcc
     1621 acgtcgagcc ggatgg
//
LOCUS       DAOPTR010000968         1633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_58453_length_1633_cov_2.570342, whole genome shotgun sequence.
ACCESSION   DAOPTR010000968 DAOPTR010000000
VERSION     DAOPTR010000968.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58453_length_1633_cov_2.570342"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(335..>1633)
                     /locus_tag="PKI05_14980"
     CDS             complement(335..>1633)
                     /locus_tag="PKI05_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA53556.1"
                     /translation="TAAGVAAVTLLSGTVPAVHAGEDHTIRLALIGCGGRGSGAVKDA
                     FSVANGPVELYAMADIFENRLQSSRKILSEMYPQQVNVEGRCFVGFDAYKHAIDCLRP
                     GDVAMLTGYAAFRPMQLEYAVSKGINVFMEKSFACDPVGVRRVIAAGKAAQEKNIKVA
                     AGLQCRHSVNRHELIRRIKDGELGEIISVRAYRMEAVGPLGPKPADVPELFWQIRNFF
                     RFFWVSGGLFSEMDIHQIDEICWIKDSWPVRAHGVGGRVANSVDRSQNFDSYFAEWTF
                     PDGTQGIDVVRYASNCYNHFATYIHGTKRAAQFSGAVHVGTVRIFKDQRIEEDNILWE
                     APKEQLTPWQAEWANLFDAIRNDKPHNEAERAALANLAAIIGRAAVHSGQIVTWEEAM
                     QSQFTFCPNIDQMTAETPPPVLPDERGFYPVPIPGQWKEV"
BASE COUNT          365 a          478 c          461 g          329 t
ORIGIN      
        1 gggttggggt gagggcaagc aatggttgga gtggatagag aaaaccggca gcaattcgag
       61 ccgccctcac ccccgacccc tctcccgccg aaacggagag gggatatggg ataggctctt
      121 aaagaaccaa gccactggcg gagccgttcg acccatcggg aaagctccac cgtcgtcgcg
      181 acccaaaagg gggacagtgc gaaattttcg atctgaaaaa cctaagtgtg gttctcgcgg
      241 ccagggtggc ctgtacgggg gtagagcggt cacctgcttc catcaacatc ttgctttatg
      301 ccggttgggg ttcgtagcca cgaggccggc ccctttatac ttctttccat tgacccggaa
      361 tggggaccgg atagaatccg cgttcgtcgg gcagaacggg tggcggcgtt tcggctgtca
      421 tttggtcaat attcgggcag aaagtaaact gcgattgcat ggcctcctcc caggtgacaa
      481 tctgcccgga atgcacagcc gcccggccaa taatggcggc caaattcgcc aaagccgccc
      541 gttccgcctc gttgtgtggc ttatcgtttc tgatggcgtc aaagagattg gcccattccg
      601 cctgccaagg cgtgagttgt tccttcgggg cctcccagag gatgttgtcc tcctcgatcc
      661 gctgatcttt gaagatgcgg accgtgccca cgtgcacagc ccccgaaaat tgcgcggccc
      721 gctttgtccc gtggatatac gtagcgaaat ggttgtagca gttactggcg taccgcacga
      781 catcgatccc ctgggtgccg tcagggaagg tccactcggc aaagtaagaa tcgaaattct
      841 ggctgcgatc gacactgttg gcaacccgac cgcctacacc gtgggcacga acaggccagg
      901 agtccttgat ccaacaaatt tcgtcaatct ggtggatgtc catctcgctg aatagtcccc
      961 ctgaaaccca gaagaagcgg aagaaattgc gaatctgcca gaaaagctcg gggacatctg
     1021 cgggtttcgg cccgagggga ccaacggctt ccatgcggta ggcccgcacc gagatgatct
     1081 cccccaattc gccatctttg attcggcgga tcagttcgtg gcgattcacg gaatgccggc
     1141 attgcaaacc ggcggccact ttgatgttct tttcctgagc ggccttacca gccgcaatca
     1201 cccgtcgcac ccccacggga tcacaggcaa acgacttctc catgaatacg ttgatcccct
     1261 tggacacagc atattcaagc tgcatggggc gaaaggccgc ataaccagtg agcatggcca
     1321 cgtcaccagg gcgcagacag tcgatggcgt gcttgtacgc atcgaagccg acaaagcagc
     1381 ggccttccac attcacctgt tgaggataca tttccgagag aatcttgcgg gaactttgca
     1441 ggcggttctc aaagatgtcc gccatggcat aaagttctac gggaccgttg gccaccgaga
     1501 acgcatcttt gacggctccc gatccacgac caccacaacc gatgagggcc aaacgtatag
     1561 tgtgatcctc gcccgcatga accgcaggaa cagtgccaga aagcagagtc acggccgcaa
     1621 ctcccgcggc tgt
//
LOCUS       DAOPTR010000969         1627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_58753_length_1627_cov_4.052799, whole genome shotgun sequence.
ACCESSION   DAOPTR010000969 DAOPTR010000000
VERSION     DAOPTR010000969.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58753_length_1627_cov_4.052799"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="PKI05_14985"
     CDS             <1..1504
                     /locus_tag="PKI05_14985"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOA53557.1"
                     /translation="SQIGSVLESMNLAGAWKLQYQDRKGQYYFRIPDNLIKKHVAPFC
                     DIPTLAYWVEAIVASGGRIENPEVALEFLFSRQLPNGAFVDAYQAEDVLESNAHVVAT
                     AQAVAVIRSLGQEVPRREQCVAWIQSCQDESGGFRWHPSYRYPGNKPDVWYTWAAVSA
                     LDRLGAKPMDIKGCLRWLNSLQNPDGGFGDRPGWNSRLYSTYYAVHALDILNASGGGA
                     RAAITAKKATAARRIIPEGKYQIYQASLKTPPGGPEMIETARKLRLNFLGVKDNSPNT
                     PPVPISVAWEHIKKQGYPMEVVSIPEQYGHQLKWIGGHPANHVANFFLPLGDSPEIKE
                     FLAAVDTAGRAGLPWPEYVQKVIQPVVQRGSLFYPELDYEMMNAYIVYDGGLEDGLGY
                     NAVVGGLGWPIWDWIRMFPYRERWVGKLPIIVDADAHGEIAKWLEPLDRQRLLYLAEH
                     YDLPHFLDACRQVRTVCVIRGGENKDELAFYGDPAVVEYVKRHQSEWQWW"
BASE COUNT          344 a          420 c          502 g          361 t
ORIGIN      
        1 ctcgcagatc ggctcggtgc tcgagtcgat gaacctggcc ggggcgtgga agcttcaata
       61 ccaggaccga aagggccagt attactttcg gattccggat aacctcatca agaaacacgt
      121 ggcgccattc tgcgatattc ccacgctggc ttactgggtc gaggccattg tggcctctgg
      181 cggccggatt gagaacccgg aagtggcttt ggaatttctc ttttcgcgac aactcccaaa
      241 cggggcattt gtggacgctt atcaggcgga ggacgtgttg gaaagcaatg cccacgtcgt
      301 agcaacggcc caggccgtgg cggtaattcg atcgcttggc caggaggttc cgcggcggga
      361 gcaatgcgtg gcatggattc aatcgtgcca agacgaaagc ggtggtttcc gctggcatcc
      421 cagctatcgt tatcccggca ataaacccga cgtgtggtac acctgggcgg ccgtttctgc
      481 cttggaccgc ctcggtgcca agccgatgga tatcaagggg tgtttgcgct ggctcaacag
      541 tttgcaaaac ccggatggcg gcttcgggga tcgaccgggc tggaactcgc gtttgtatag
      601 cacatattac gcggtccatg ccttggatat tctcaatgct tcgggtgggg gcgcgcgggc
      661 ggcgataacg gccaaaaagg caaccgccgc gcggaggatt attcctgagg gcaaatatca
      721 aatttatcaa gcatcgctaa agactccgcc gggtgggccg gaaatgattg aaacggccag
      781 aaaattgcgg ctcaacttct tgggcgtcaa ggataatagc ccaaacaccc cgcccgtgcc
      841 tatttctgtg gcctgggagc acatcaagaa acaaggttat cccatggaag tcgtttccat
      901 tccggagcag tacggccatc aattgaagtg gattggaggc catccggcca accacgtggc
      961 gaatttcttc ctgccactgg gcgattcacc tgagatcaag gagtttttgg cggctgttga
     1021 tactgcgggt cgggcgggtc ttccctggcc ggagtacgtc cagaaggtga ttcaacctgt
     1081 cgtccaacgg ggctcgctct tctatcccga attggattac gaaatgatga acgcctatat
     1141 cgtctatgac ggtggtttgg aagacggttt aggatataac gcggtggtgg gtgggcttgg
     1201 ttggccgatc tgggattgga ttcgcatgtt tccctaccgt gagcggtggg tgggcaaact
     1261 gccgatcatt gtcgatgccg acgcccatgg cgagatcgca aagtggctgg aaccgcttga
     1321 ccgccagcgt ctgctgtatt tggcggagca ttatgacctt ccgcattttc tggatgcttg
     1381 ccgtcaggta cgaacggtgt gcgtcatccg gggcggcgag aacaaggatg agctcgcctt
     1441 ttacggagac ccggccgtgg tcgaatatgt caaacgccac caaagcgagt ggcagtggtg
     1501 gtagcgcccg cctgaattaa gagcctatcc caaagttccc tctccctctg ggagaaggtt
     1561 ggggtgaagg cgagcgatcg tcggagtcaa tcgagaaaac cggtaacgat tggagtcacc
     1621 ctcaccc
//
LOCUS       DAOPTR010000970         1627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_58761_length_1627_cov_3.061705, whole genome shotgun sequence.
ACCESSION   DAOPTR010000970 DAOPTR010000000
VERSION     DAOPTR010000970.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58761_length_1627_cov_3.061705"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="PKI05_14990"
     CDS             <1..567
                     /locus_tag="PKI05_14990"
                     /inference="COORDINATES: protein motif:HMM:NF016563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HOA53558.1"
                     /translation="FLFTQDGDLNGLIEGEQHNTYDINFYGPNTMVGSLYLAACRAGE
                     EMARELGDAEFAARCRKVFEAGREATMKELFNGEYFIQKVDLKEHPKHQYADGCLSDQ
                     LFGQGWAHQVGLGYIYPPEAVRSALKAIWVYNWAPDVGPQNEAHPPQRWFARPGQAGL
                     FTCTWPKSKHLGPDSVLYRDEVWTGIEY"
     gene            580..1092
                     /locus_tag="PKI05_14995"
     CDS             580..1092
                     /locus_tag="PKI05_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53559.1"
                     /translation="MVWEGMLLEALAICRGVHERYHPRSHNPWNEVECGDHYARAVAS
                     YGVFLALCGFEYHGPKGHIGFAPRMNADDFAAAFTAAEGWGTFTQKRQGNTQENTLQV
                     LWGRLRVNSIALEAPGENRVKAVVRIKSGTGEKEVGVSRMEQTGRRVVIYLAVPLQLE
                     TGQTVTVHLT"
     gene            1137..1526
                     /locus_tag="PKI05_15000"
     CDS             1137..1526
                     /locus_tag="PKI05_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53560.1"
                     /translation="MSFATVVNCMDGRTQGPVISYLKERFGVDYVDNSTEAGPDGLLA
                     RFDDPATVEAICRKIRISQEKHGSRWLAIVGHADCGGNPVSPEEHHRQIRAAVEFLRQ
                     RFPTMTVIGLWLGEDWIVQEIAGEEGG"
BASE COUNT          352 a          412 c          516 g          347 t
ORIGIN      
        1 ttcctcttta cccaggatgg cgatctcaat ggcctaatcg agggtgagca gcacaacacg
       61 tacgacatca acttttatgg gccgaacact atggtcggtt cgctctattt ggcggcgtgc
      121 cgtgccggcg aagagatggc ccgagagctt ggcgacgcgg aattcgccgc gcgttgtcgt
      181 aaggtatttg aagccggccg cgaggccacc atgaaggagc tatttaacgg cgagtacttt
      241 atccaaaagg tggacctcaa ggagcatccc aagcatcaat acgcggacgg atgtttgtcg
      301 gatcagttat tcgggcaggg ctgggcccat caagtggggc tcggttacat ctatccgccc
      361 gaggccgtca ggtccgcatt aaaggcgatt tgggtttaca attgggcacc ggacgtcggg
      421 ccgcagaatg aggcacatcc gcctcagcgg tggttcgccc gaccaggaca ggcggggctt
      481 ttcacgtgta cttggccgaa atcaaagcac cttggaccag atagcgtttt gtaccgcgat
      541 gaggtttgga cggggatcga atattaagtt gctggccaca tggtatggga aggcatgcta
      601 ctcgaggcct tggcgatttg ccgcggtgtt cacgagcggt atcatcctcg cagtcataat
      661 ccgtggaacg aagtcgaatg cggtgaccac tacgcgcggg cagtcgccag ctacggcgta
      721 ttcctcgccc tgtgcgggtt tgagtatcat ggccccaaag gccatatcgg atttgccccg
      781 cggatgaatg ccgatgattt cgcggcagcc tttacggcgg ccgaaggttg gggcacgttc
      841 acgcagaaac gtcaagggaa tacgcaggaa aacacacttc aagtcctgtg gggacgcctt
      901 cgcgtgaaca gcatcgcctt ggaagccccc ggcgagaacc gagtcaaagc cgttgtgcgg
      961 ataaagtccg gcacgggcga gaaagaggtg ggcgtttctc gcatggagca aaccggccgg
     1021 cgcgtggtga tctatctggc agtacctctc cagttagaga ccggccagac tgtgactgtt
     1081 cacctgactt gatcgggatt aagcgacagc aggtatcttg gaaagggcta aaggccatga
     1141 gttttgccac tgttgtcaac tgcatggatg gccgaacaca agggcctgtg atcagttatc
     1201 ttaaagaacg gttcggtgtg gattatgtgg ataacagcac ggaagcgggc cccgatgggc
     1261 ttttagcccg gttcgatgac cccgctacgg ttgaggccat ttgtcgcaag attaggattt
     1321 ctcaggagaa acacggctca agatggctgg ccattgtcgg gcacgccgat tgcgggggga
     1381 atcccgtctc gccggaggaa catcatcggc aaattcgggc tgccgtggaa ttccttcggc
     1441 aaaggtttcc caccatgacg gtcatcggtt tgtggttagg ggaagattgg atcgtccaag
     1501 agatcgccgg tgaagaaggt ggataagagg ctatcccaca acgtcccctc gcccgttcgg
     1561 cgggagaggg gacggggtga gggtggctcg aatcgctgct ggttttctcg atcgactccg
     1621 acgatgc
//
LOCUS       DAOPTR010000971         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_58958_length_1623_cov_3.902423, whole genome shotgun sequence.
ACCESSION   DAOPTR010000971 DAOPTR010000000
VERSION     DAOPTR010000971.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58958_length_1623_cov_3.902423"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="PKI05_15005"
     CDS             <1..948
                     /locus_tag="PKI05_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53561.1"
                     /translation="KDVFTCQGPAMKVGDGPWAWLGREKVRDTSFSVVLPHDGSEVRF
                     CLAFPYLEEDLRRFLNRHAGHPHLRVTEHCVTPKGRRTERLHLGCLDKTPPHRVLLAC
                     RHHACEMMASWVLEGLMEAVLTGPDASWFRNNVEFAIVPFMDKDGVEDGDQGKNRRPH
                     DHNRDYQDEPIYPSVRAMKEFGPRWSEGRLRVAMDLHCPSIRGGRNEEVFLVGSPHEA
                     IQTQIDRFAEILRAKSADTIPYDPRNNIPFGVEWNNTSPERSRTFARWASSLEGINLA
                     CTLEFPYACAGGKVVDAASSRAFGTSLAAALKQYLETLG"
     gene            1299..>1623
                     /locus_tag="PKI05_15010"
     CDS             1299..>1623
                     /locus_tag="PKI05_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53562.1"
                     /translation="MAQKIPPQTPSPSDCEGWPPESAEGPSGIFQDETVAEGVPTLLD
                     LIPRHGQPLGMLLTGIAAVALLLAGYRGFGADTPAWVPTPLPSFGLEGPGNLASWFST
                     LVLTAA"
BASE COUNT          323 a          471 c          492 g          337 t
ORIGIN      
        1 aaggatgttt tcacgtgcca aggtcccgcc atgaaggtcg gcgacggccc gtgggcttgg
       61 ttgggacgcg aaaaagtgcg ggacacatcc ttcagcgtgg ttcttcctca cgatggctcc
      121 gaggtccgtt tttgcctggc ctttccgtac cttgaggaag accttcgtcg cttcctgaac
      181 cggcatgcgg ggcatccgca tttgagggtc acggagcatt gtgttacacc caaaggtcga
      241 aggaccgaac gacttcacct aggctgcctc gataagacac cgcctcatcg tgtcctcttg
      301 gcctgtcggc atcacgcctg cgagatgatg gccagttggg tgttggaggg gctgatggag
      361 gccgtcctca ctggccccga cgcctcctgg tttcgtaaca atgttgaatt cgccatcgtc
      421 ccgttcatgg acaaggatgg tgtcgaagac ggcgatcaag ggaagaaccg gcgtccgcac
      481 gaccacaacc gcgactacca agacgagcca atttacccct ccgtccgcgc gatgaaggag
      541 tttggcccgc gttggtcaga aggccgcctc cgcgtggcca tggacctgca ttgcccctcg
      601 atccgcggtg gtcgtaatga agaggtcttc cttgtcggga gtcctcatga ggccatccaa
      661 acccagatcg accgatttgc ggagattctc cgagcaaagt ccgccgatac cattccctac
      721 gacccaagga ataacatccc ctttggtgtg gaatggaata acacatcgcc cgagcggagc
      781 cgcaccttcg cccgttgggc ctccagttta gaagggatca acctcgcttg tacccttgaa
      841 ttcccctatg cctgcgccgg cggaaaggtg gtcgatgccg catccagccg ggcatttggg
      901 acgtcgcttg ccgcggccct gaaacagtac ctggagacgt tgggatagcg ccggctacgt
      961 tgacccgccg atagtgaccg gcgggctacc acaagggttg cctctatgaa tgggcgtccg
     1021 tccgaagatg aataccgcca gcacctgcgc tttgggtatt taggatcggc tgagtagaat
     1081 agaggattgg accaaactat tcccaaaata gcgatcatat cagccttgat tcctttacga
     1141 gcgatctatt cggactttgg ccaaggccgc tgcacgagcg gagttaggcc ggacgaaaca
     1201 aactatgacg ggacgatcat gcgccggcgg agcgctgcgc caatggggga cgaccgcggg
     1261 tccgaggtgg cggcggttga tgtttgagga tgaaccggat ggctcaaaag attccacccc
     1321 aaactccgag cccgagtgac tgtgagggtt ggccacccga atcggcagag gggccgtccg
     1381 gtatctttca agatgaaacg gtggcagagg gtgtgccaac gcttctcgac ctaattcctc
     1441 ggcacggtca accgctgggg atgctgctga cgggcattgc ggctgtcgcc ttattgctgg
     1501 ccggttatcg tggttttggg gcggacaccc cagcttgggt cccgacgcca ctgcccagct
     1561 tcgggttgga aggaccggga aacctggcca gttggttttc caccctggtc ctcaccgccg
     1621 cag
//
LOCUS       DAOPTR010000972         1622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_59017_length_1622_cov_3.381621, whole genome shotgun sequence.
ACCESSION   DAOPTR010000972 DAOPTR010000000
VERSION     DAOPTR010000972.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59017_length_1622_cov_3.381621"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..1108)
                     /gene="rfbG"
                     /locus_tag="PKI05_15015"
     CDS             complement(32..1108)
                     /gene="rfbG"
                     /locus_tag="PKI05_15015"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR02622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HOA53563.1"
                     /translation="MNPRFWQGRRVFLTGHTGFKGGWLSLWLQSLGAELTGYALAAPT
                     TPSLFEEARVAQGMHSIEGDIRDLARLQEAVKSARPEIVIHMAAQPLVRYSYHYPVET
                     YATNVMGTVHVLEAVRHTPGVRAVVVITTDKCYDNREWVWGYREGDPMGGYDPYSNSK
                     GCAELVSSAYRSSFFNPAEFPAHRVALATARAGNVIGGGDWAADRLIPDIVAAFEAGR
                     PVHIRNPGAVRPWQHVLEPLRGYLMLAERLYEDGPAFAEGWNFGPGDEDAKPVGWIVE
                     QMAARWGELARWQLDQSEHPHEAAYLKLDISKARAQLRWHPALRLDGALGLVIDWAKR
                     RRSGDDVRALTQEQIDHYQRLTKN"
     gene            complement(1105..>1622)
                     /locus_tag="PKI05_15020"
     CDS             complement(1105..>1622)
                     /locus_tag="PKI05_15020"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOA53564.1"
                     /translation="RYAEPWRVTLVDTGDNTMTGGRLKRVAAYLEGEELFCFTYGDGV
                     SDVNIAKLIDFHRAHGLQATLTATYPPGRFGAIDIHSDHRVTSFKEKPKGDGGMINGG
                     FFVLSPKVLSLIGGDDTRWEQEPLESLAESGELKAYSHEGFWQPMDTLRDKNLLEDLW
                     ASNKAPWRVWD"
BASE COUNT          337 a          510 c          459 g          316 t
ORIGIN      
        1 ttggcgcttg tctgttcgtt ctgcatggtt cctaattttt ggttagtcgt tggtaatggt
       61 cgatctgctc ttgagtcagg gcacgcacat cgtctccaga acgacgccgc ttggcccaat
      121 cgatgacgag gccaagagca ccgtccaaac gaagcgccgg gtgccatcga agttgcgctc
      181 gcgccttgga aatgtcgagt ttcaggtagg ccgcctcatg aggatgctcg gattggtcga
      241 gttgccatcg ggcaagctca ccccagcgcg ccgccatttg ctcgacaatc cagccgacag
      301 gcttggcatc ctcgtcaccg gggccaaagt tccatccttc ggcgaaagcc ggaccatcct
      361 catacagacg ctcggcaagc atcagataac cccgcagggg ctcgagcaca tgctgccagg
      421 gacgtaccgc gccgggatta cggatatgaa cgggtcggcc tgcttcaaat gccgccacga
      481 tatccggaat caagcgatct gccgcccagt cgccgccacc gatgacattg ccggcacggg
      541 ccgtggccag agcgacacgg tgcgccggaa actccgccgg attgaagaac gacgagcgat
      601 aggcggaact cacgagttcc gcacaaccct tgctattgct gtagggatcg taccccccca
      661 tggggtcgcc ctcgcgatag ccccagaccc attcccggtt gtcgtagcac ttgtcggtgg
      721 tgatgacaac gacggccctg acgccgggag tgtggcgcac cgcctccagc acatgaaccg
      781 tgcccatgac gtttgtggcg tatgtttcca ccgggtaatg gtacgagtag cgaaccagcg
      841 gctgggcggc catgtggatc acgatctccg gccgagccga ctttaccgcc tcctgcaaac
      901 gagccaagtc acgaatatcg ccctcgatcg aatgcatgcc ttgagcgacg cgcgcctcct
      961 cgaaaaggct tggggtcgtt ggcgccgcga gcgcatagcc agtaagctcc gcgcccaggc
     1021 tttgcagcca aagcgaaagc caaccgccct tgaacccggt gtgtccggtt aaaaagacgc
     1081 gcctcccctg ccaaaaccgc ggattcaatc ccagaccctc cacggcgcct tgttgctcgc
     1141 ccacagatct tcaagaaggt ttttgtcccg caacgtgtcc atcggctgcc agaatccttc
     1201 gtgggaatag gccttgagct ccccggattc cgccaacgac tccaggggct cctgttccca
     1261 tcgagtatcg tcgccgccga taagagagag aactttaggc gagagcacga aaaagccgcc
     1321 gttgatcatg ccaccgtcgc cttttggctt ctccttgaac gaggtgacgc gatgatccga
     1381 gtgaatgtcg atagcgccga agcgacccgg tgggtaggtt gcggtcaggg tcgcctgcaa
     1441 cccatgagca cgatgaaaat caatcagctt ggcgatattt acgtcactga caccgtcgcc
     1501 ataggtaaaa cagaacagtt cttcaccttc aagataggcg gccacccgtt tcagccggcc
     1561 accagtcatg gtgttgtcac cggtatcgac cagggtcacc cgccaaggtt cggcgtagcg
     1621 ct
//
LOCUS       DAOPTR010000973         1622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_59018_length_1622_cov_3.362476, whole genome shotgun sequence.
ACCESSION   DAOPTR010000973 DAOPTR010000000
VERSION     DAOPTR010000973.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59018_length_1622_cov_3.362476"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PKI05_15025"
     CDS             complement(<1..745)
                     /locus_tag="PKI05_15025"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HOA53565.1"
                     /translation="MPEHAVFVVGFGFAFLFLGYLLYGRFLGKRVFHLKGDAPTPAHT
                     MYDGVDYCPAPTPVLFGHHFASIAGLGPILGPALAVIWGWFPAVVWVLVGAVFIGAVH
                     DLGALVVSLRHRGRSVGEVAHYVLGPRARLLALLIIFFLMSLAMGAFCNTLADLFLNF
                     NPDAIVPSVGLMILAVLFGLSVYRLKAPLALATIVALLIFAGLIYLGEKVPVLSYQWL
                     CSAETQQAINQAKDATAPSGGPTFARPYGA"
     gene            1057..>1622
                     /locus_tag="PKI05_15030"
     CDS             1057..>1622
                     /locus_tag="PKI05_15030"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA53566.1"
                     /translation="MAYLSSRRSFLKRVSAAATCLILPSSRIAFSYEANEKVHYAAIG
                     LGKRGSYLAPTFARIGGELVVLCDADQERIGPFAQKFPKGSVYRDFRRMFDECEKSID
                     AVVIATPDHTHAVISAAAIRRGKHVYCEKPIAHDVREARVLRELAAQKKVATQMGNQG
                     MATDSFRRTLEQLEDGMIGHIREAHIWFV"
BASE COUNT          427 a          430 c          441 g          324 t
ORIGIN      
        1 ttgcaccgta gggccgcgca aaagtgggac cgcccgatgg agcggttgca tctttggctt
       61 ggttgattgc ttgttgtgtc tcagcggaac ataaccattg gtagctgagc acggggacct
      121 tttcgccgag atagatcaat cccgcgaaga tcagcagcgc aacgatcgtt gccagcgcca
      181 aaggggcctt caatcgatag accgataggc caaaaagaac agcaaggatc atgagaccga
      241 cagaaggcac aatcgcgtca ggattgaagt taaggaagag gtcagccaac gtattgcaaa
      301 aagcacccat cgccagcgac atgagaaaga agataatgag tagtgccaaa agccgcgccc
      361 gcggaccaag cacataatgc gcgacttctc ccacggatcg cccacggtga cgcaaactca
      421 cgaccagcgc ccccagatcg tgaaccgctc cgataaagac ggcgccgacc aaaacccaga
      481 cgactgcggg aaaccaaccc caaatgacgg ccaacgccgg tcctaaaata ggccctaatc
      541 cggcgatgga ggcaaaatga tgaccgaaaa gcacaggcgt cggcgcgggg cagtagtcaa
      601 cgccgtcgta catcgtatgt gcgggcgtcg gagcatctcc cttcaggtga aaaactctct
      661 tgcccaagaa ccgtccataa aggagatatc ccaaaaacag gaaggcaaaa ccgaacccga
      721 caacaaacac ggcatgttca ggcatagcgg ggcctcgtga tcggaggaaa accgagtgtc
      781 ctgtaaccca tccgaatcag aaacggtgtt ctcgggaaac ttcgttttta acttctgcat
      841 ccagcatagt tttttgctga atttctttca atcacaacct aacgctcccc cactttctca
      901 tacctaacca gttggtcatg ggtgcaggtc atggcagatg cactctcgcg cagtggacat
      961 tctcggtcaa gacggtacaa cattatttgc agatgcggca ttgttcgttg ggttgaccct
     1021 ccctttcgga attacctaac ccaaggagta tcgaccatgg cctacctgtc gagtcgtcgg
     1081 agcttcctaa agcgtgtgtc ggctgccgcc acttgcctaa tcttgccgtc gtccaggata
     1141 gctttttcct acgaagccaa cgaaaaggtt cactacgcag ccattggact cgggaaaagg
     1201 ggaagttacc tggcgccgac atttgccagg attggaggtg aattggtcgt gctttgcgac
     1261 gcagatcagg aaagaatcgg gccctttgcg cagaagttcc caaagggatc agtttatcga
     1321 gatttccgcc gcatgttcga tgagtgtgag aagagcattg atgctgtggt gatcgccacg
     1381 ccagaccaca cacatgccgt tatttcggcg gcggcgatcc gccggggaaa gcacgtctat
     1441 tgtgaaaagc cgattgccca cgatgtccgc gaggcacgtg tcctcagaga attggccgca
     1501 cagaaaaagg ttgctacgca gatgggcaat cagggaatgg cgacggactc attccgcagg
     1561 acgttggaac aacttgagga tgggatgatt gggcacattc gagaggccca tatttggttt
     1621 gt
//
LOCUS       DAOPTR010000974         1619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_59186_length_1619_cov_4.267903, whole genome shotgun sequence.
ACCESSION   DAOPTR010000974 DAOPTR010000000
VERSION     DAOPTR010000974.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59186_length_1619_cov_4.267903"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1540
                     /locus_tag="PKI05_15035"
     CDS             <1..1540
                     /locus_tag="PKI05_15035"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOA53567.1"
                     /translation="LVFLGLALLVWDCIEVGRNDASNIVNAVFGARILNRRTAVRVAG
                     LAVVIGASAASPVFETARKGIFDPGMLTLEQAVIAYISVYLVDTVLLFTYSAFGMPVS
                     TTACLIFELVGASVGLYGLSVVHWGKVGTVIAAIFVSIFMTGLAGFLIQRVFRAAIGT
                     KIDDRETILLHGPWISGFMLTGLGWFMVFKGMSGIPFVNAFREYAIDPYIDAFTPTFA
                     FLSAWGLFTLIVHLFLSFSGTIGTRYLFPAMAVLGMICLAFAFGQNDLANCASPGISA
                     FWLWRHAEQTVAQATQITIPTWVLFICGCLLVAGMMTENAQRVTRAQVNVGSQFDRVA
                     LYAPEWCRAMARALLRFFPHRPELAPPPMVSPQGKKVHYDALRAAVISSVSAGVIALA
                     SSRGLPVSTTYVAFAAVIATGLADRVLARGDADLKIGRAIWVVVSWFLAAIIAVVATA
                     GVARLIYHLGFVGLLSALAINLSVRFYSQKVADEQERRIHRHRGGQPPVDQLAKTEIH
                     ADVP"
BASE COUNT          281 a          429 c          461 g          448 t
ORIGIN      
        1 tctcgttttt ttgggattgg cactgctcgt ctgggactgt attgaggttg gccgtaatga
       61 tgcttcgaac attgtgaatg cggttttcgg ggcacggatt ctcaatcggc gaacggcggt
      121 ccgtgtcgct gggttggctg tggtgattgg ggcctcggcc gccagtcccg tcttcgaaac
      181 agcccgcaag ggtatctttg atcccggcat gttgaccctt gagcaggccg tcattgccta
      241 cataagcgtc tatttggtgg atactgtgct cctttttacc tactccgcct tcgggatgcc
      301 tgtgagcact acggcatgtt taatctttga gttggtaggt gcttcagtgg ggctttacgg
      361 tctctcagta gttcattggg gtaaggtggg aacagtcata gcggccattt ttgtttcgat
      421 tttcatgacg gggcttgccg ggttcctgat ccagagggtc tttcgcgcgg ccattggaac
      481 caagatcgac gatcgcgaaa caatactcct ccatgggccg tggatttccg gttttatgct
      541 taccggtttg ggatggttta tggtgttcaa agggatgtca ggcattccct tcgttaatgc
      601 atttcgggag tacgcaattg acccctatat tgatgccttc acaccgacct ttgccttttt
      661 gtcggcatgg ggcctgttta ccctgatcgt ccatctcttt ctctccttct caggaaccat
      721 cgggacgcgg tatctttttc cagctatggc ggtgctggga atgatatgcc tggcatttgc
      781 ctttggccag aacgaccttg ccaattgcgc gtcgcccggc atttcggcat tttggctttg
      841 gcgccacgcg gaacagacgg ttgcacaagc gacacaaatt accattccca cgtgggtgct
      901 attcatatgc ggttgtcttc tcgtcgccgg aatgatgacc gaaaacgcac aacgtgtcac
      961 ccgggcgcaa gtgaatgtgg ggagccaatt tgaccgggta gcattgtacg ccccggaatg
     1021 gtgtcgcgca atggcccggg cgctattgag gttctttcct caccgacctg aattggcccc
     1081 gccccccatg gtaagtcccc aggggaaaaa ggtccattat gacgccttgc gggcagccgt
     1141 cataagttcc gtcagcgccg gggtgattgc cctggcgtcc agtcgtggcc ttcctgtttc
     1201 caccacgtac gttgcctttg ccgcagtcat cgccacgggt ttggcagatc gtgtgctggc
     1261 tcgcggcgat gccgatctca agatcggtcg ggccatttgg gttgttgtca gttggttttt
     1321 agctgcgatt attgcggtag ttgctacggc cggggtcgca cggttgattt atcacctcgg
     1381 tttcgtcgga cttctcagcg cacttgcaat caatctttcc gtacggttct actcacaaaa
     1441 ggtagcggat gagcaggaac gccgaattca ccgccaccgc ggaggccagc cgccggtcga
     1501 tcagcttgcc aagaccgaga ttcatgccga tgtaccgtga tggaacccca cgcgtgcctc
     1561 aaagttccca tcctttgcga ttttctcgtg ttcgcagagc gttggcctct gaggaattg
//
LOCUS       DAOPTR010000975         1619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_59200_length_1619_cov_2.991688, whole genome shotgun sequence.
ACCESSION   DAOPTR010000975 DAOPTR010000000
VERSION     DAOPTR010000975.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59200_length_1619_cov_2.991688"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..>1619)
                     /gene="groL"
                     /locus_tag="PKI05_15040"
     CDS             complement(165..>1619)
                     /gene="groL"
                     /locus_tag="PKI05_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911952.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOA53568.1"
                     /translation="AKEIELEDRFENMGVKLVKEVASKTSDVAGDGTTTATILARAIY
                     REGLRNVIAGSNPTAIRRGIDRAVEAAVSHLMSMARKVQNKAELASVGAISANNDRKI
                     GELLAEAMEKVGKDGVITVEEGKTAETTYKIVEGMQFDKGYLSPYFINRVPEMDCYME
                     DAYILIHEKKISNLRELIPLLEKVAHSGKPLLIIAEDVEGEALTALVVNKLRGILQVC
                     AVKAPGFGDRRKAMLGDIAILTGGTLISEDLGMKLENIELSHLGRAKKVTVDRDKTTI
                     VEGAGKPADIKARCDQLRHLIETTDSDYDREKYQERLAKLSGGVAVIQVGGATEAEMK
                     QTKARVEDALHATRAAAEEGIVPGGGVALLRCREAVEKARASARGDEKIGVDIVYRAL
                     SKPLEQIAENAGLDGSVVVQEVLEKDTNVGFDALTKKYVDMFKAGIIDPTKVVRCALE
                     NAASIAGLMLTTEVLVTDIPKEDEEESVTEGVVR"
BASE COUNT          329 a          520 c          389 g          381 t
ORIGIN      
        1 cccgttctcg tcactctaat ctaatgtacc acagcgcgaa aaccatcgaa ccccgaatgt
       61 gaaccaaatt gctatctatt acagggaatc gggccatccc tcactgctta agggatggcc
      121 ctttttacgc cacggctccg atgggtcatc aacctagaag gttctcaccg cacaacgcct
      181 tcagtaacgc tctcttcctc gtcctccttg gggatgtctg tgacgaggac ctcggttgtc
      241 agcatcaggc cagcaatgct cgccgcattt tcgagggcac aacggacaac ctttgtcgga
      301 tcgataatcc cggccttgaa catgtcaaca tactttttgg tcaaggcgtc gaatccgaca
      361 ttggtgtcct tttcaagcac ctcctgcaca accacggagc catccaagcc agcgttctcg
      421 gcaatctgct ccagtggctt gctcaaagcc cgataaacga tatccacacc aatcttttcg
      481 tcgccgcggg cactagcgcg ggccttctcc acagcttccc gacaacgcaa cagtgccacg
      541 ccaccaccgg ggacaattcc ttcctcggca gcagcccggg tggcatggag ggcatcctcc
      601 acccgcgcct tagtctgctt catttcggcc tcggtagcac cgccaacctg aatgacggcc
      661 accccaccgc tcagctttgc cagccgctct tggtattttt cgcggtcata gtcactgtcg
      721 gtggtttcga tcaggtgacg gagttgatca cagcgggcct tgatgtccgc gggcttaccc
      781 gccccttcaa cgatcgttgt cttgtcacga tcaaccgtca ccttcttcgc ccgaccgagg
      841 tgggatagct cgatgttctc gagcttcatc cccaagtctt cgctgatcag cgtaccgcca
      901 gtaaggatgg cgatgtcgcc caacattgct tttcgccgat caccgaagcc aggggctttc
      961 accgcgcaga cctgaagaat tccccggagc ttgttgacca ccagagcggt cagggcctcg
     1021 ccctccacat cctcggcgat gattaacaag ggcttgccac tatgggcaac tttctccaaa
     1081 agcggaatca actcgcggag attagaaatc ttcttttcat gaatgaggat gtacgcatcc
     1141 tccatgtagc aatccatctc ggggacgcgg ttgataaagt agggcgagag gtagccctta
     1201 tcaaactgca tcccctcgac gatcttataa gtggtctcag cagtcttgcc ttcttccact
     1261 gtgatcacgc catccttgcc caccttctcc atggcttcgg cgagaagctc accgatcttc
     1321 cggtcgttat tggcactaat ggcgccgacg ctggccagct cggccttgtt ttgaaccttc
     1381 cgggccatgg acatcaggtg agaaaccgcc gcctcgacgg cgcggtcaat cccacggcgg
     1441 atcgcagtcg gattgctccc ggcaattacg tttctgaggc cttcccgata aatcgcgcgt
     1501 gccagaattg ttgccgtcgt cgtcccgtca ccggccacat cggaggtctt ggacgccact
     1561 tccttaacaa gtttgacgcc catgttttcg aagcgatcct caagttcaat ctccttggc
//
LOCUS       DAOPTR010000976         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_59348_length_1616_cov_2.975016, whole genome shotgun sequence.
ACCESSION   DAOPTR010000976 DAOPTR010000000
VERSION     DAOPTR010000976.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59348_length_1616_cov_2.975016"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /gene="scpB"
                     /locus_tag="PKI05_15045"
     CDS             <1..452
                     /gene="scpB"
                     /locus_tag="PKI05_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOA53569.1"
                     /translation="ALLRRLNKLYDEKRMAFRAIEVAGGFQLRTRACFEPWIARLVKD
                     PHQTRLSPPALETLTVIAYRQPILRAEIESIRGVQCGEVIRQLLERGLIRVVGRSEEL
                     GRPYLYGTTARFLEMFGLRGLDDLPHPEYRIPSVALRRPVAEASNGG"
     gene            652..1044
                     /locus_tag="PKI05_15050"
     CDS             652..1044
                     /locus_tag="PKI05_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53570.1"
                     /translation="MRKELTQRLLARVGVLEDKGCPSRRNDSCACVLMENAPSIVAQD
                     EDEDWDDDEDWDDEEDEEEDWDDEDEDWDEDEDEDWDDEDEDEDWEDEDLDEDDEDWE
                     DEDLDEDEDWDDEEDEEDWDDEDLEEDE"
     gene            complement(1304..>1616)
                     /locus_tag="PKI05_15055"
     CDS             complement(1304..>1616)
                     /locus_tag="PKI05_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53571.1"
                     /translation="RIRIDGDTGRLNAVATASEDGRRVVVKIVNPTEVTHTLRVQSAG
                     GRIAAAQAVQVAADRLMARNTLDNPSLIRPQTLQTQLTSEGATVVVPRYGCVIVEMEL
                     K"
BASE COUNT          393 a          368 c          497 g          358 t
ORIGIN      
        1 gggcgttgtt gcgacgactc aacaagttgt atgatgaaaa gaggatggcg tttcgcgcaa
       61 tagaggttgc cgggggattc caactacgga ctcgggcatg ttttgagcca tggatcgccc
      121 ggctggtgaa ggatccgcac cagacgcgac tgtcgccacc cgctttggag acactgacag
      181 ttatcgccta tcgtcagcca atcttacggg cggagatcga atcgattcgg ggagtccaat
      241 gtggcgaggt gattcgacag cttctggaac gtgggcttat ccgcgtggta gggcggtcgg
      301 aggaattggg acgcccttac ctttatggga caacagcacg ttttttggag atgttcggac
      361 tgaggggact ggacgatttg ccacatcctg aatatcgtat tccatcggtt gcgttgaggc
      421 gtcctgtggc cgaagcatca aacggtggat gaacaacctc ttatcgttag gccaaaagga
      481 cgatatactt gacgtttcgg cacgtttggc tggattgacg ataagtgcgt gggtagtcga
      541 aaacctattt gaatattgcg gagacaagaa ccgcggagag gtcaccaagg ccaggctttc
      601 cgcgtttcgg aatcaactgt ttggccttag ttttttgtca ggggtgtcac cgtgagaaaa
      661 gaattgactc aacgcttatt ggctcgagtg ggtgtcttgg aggacaaagg ctgcccaagt
      721 cgcaggaatg attcctgtgc ctgcgttctg atggagaatg ccccatcgat cgtcgcccaa
      781 gatgaagacg aggattggga cgacgatgaa gattgggacg acgaagagga tgaggaagaa
      841 gactgggacg acgaagacga agactgggac gaggacgaag acgaggattg ggacgacgaa
      901 gacgaagatg aagactggga ggatgaggat ttagacgaag acgacgaaga ttgggaggac
      961 gaagaccttg acgaagacga ggattgggac gacgaagagg acgaagagga ttgggacgat
     1021 gaagatcttg aggaggacga gtaagcacta aaagtcctcg ccgcgtccaa tcgccggatg
     1081 tcttgcgagt cagaccgcgt tatagagttt gaacggccga cagacggttt cacaattgtg
     1141 gacagtggtt acgagaaggt gtaaccactg tccatctttt gttggcaaaa gctactgtcg
     1201 ggcggccttc tcgattttca ggagtcgatc gagcaattga ggccgacgct cctaaccgaa
     1261 acggtcggca tgcctcctca ggttagtaac ctgaaggagc tcgtcatttc agttccatct
     1321 cgactatcac gcacccatac ctcgggacaa ccactgtggc cccttcgctg gtcaactgtg
     1381 tttgtagtgt ctgagggcga atcaacgagg gattatctaa ggtgttacga gccattaaac
     1441 ggtctgccgc gacctgaact gcttgggcag ccgcaatcct ccctccagca ctttggacgc
     1501 ggagtgtgtg ggtaacctcg gttgggttaa caattttcac gaccactcgc cttccatcct
     1561 ctgaggcggt cgccaccgca ttgaggcggc ccgtatctcc atcaatgcga atccga
//
LOCUS       DAOPTR010000977         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_59357_length_1616_cov_2.398463, whole genome shotgun sequence.
ACCESSION   DAOPTR010000977 DAOPTR010000000
VERSION     DAOPTR010000977.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59357_length_1616_cov_2.398463"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="PKI05_15060"
     CDS             complement(<1..941)
                     /locus_tag="PKI05_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53572.1"
                     /translation="MKTSTVDSQQTSLLPVQRQVLFLMIAVSAGIMLGRIIAVDSVDV
                     YQLERRRWEEIERRVEVRRSELVAKGVTGASLEKELDRIRKALESEARLRRPFLSAND
                     RSRWCTVRALVEPAMRVPGAPYAIDRVIQERLWDTIDMVMHDGHLYSSKPPLFATLVA
                     GEYWLLYHGLGWTLKDHPYQVAWVVLLTFNWVPLLIFFAVLVRLAQRLTDNFWVQAFV
                     LASACFGTYLTTFAVTLNNHLPAAVCAAVGLELACQILRDGQREAWKFVLLGLVATLG
                     VTFELPSLVAWVILAFFLFAKAPGRTALWFVPASCLVA"
     gene            complement(1169..>1616)
                     /locus_tag="PKI05_15065"
     CDS             complement(1169..>1616)
                     /locus_tag="PKI05_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA53573.1"
                     /translation="PVLAANRGFKVSEIVIHHRPRRYGRSKYGFGRFIKGFLDLLTVR
                     FLTGFGTRPQHFLGSIGLVAFLVSALILTFLALEWTISRIIPGMDVVYLRDRPLVIYS
                     VGLLLLGAQLMSVGFLAELLTAYHIQHEKTYSLADEIGTNGGPPST"
BASE COUNT          408 a          455 c          448 g          305 t
ORIGIN      
        1 gccacaagac aacttgccgg cacgaaccag agtgctgtcc ttcccggggc cttggcaaaa
       61 aggaaaaacg ccaagatgac ccatgcaaca agcgacggca gttcaaaggt cacacccaag
      121 gtggcgacaa gccccaggag gacgaatttc cacgcttctc gttggccatc tctcagaatc
      181 tggcaagcaa gctccaggcc cacggccgcg cacactgctg ccggcagatg gttatttaac
      241 gtcacggcaa atgtcgtcag ataggtccca aaacaggcac tggcaagcac gaaggcttgg
      301 acccagaaat tgtccgtcag tcgctgggcc aaccggacca gaacggcaaa aaagatcaag
      361 agcggcaccc agttgaatgt caaaaggacc acccaggcca cctgatacgg gtgatctttg
      421 agggtccatc ccagtccgtg gtagagtagc cagtattcgc ccgcaactaa cgtcgcaaat
      481 aacggcggct tgcttgaata gaggtgtccg tcgtgcatca ccatatcaat cgtgtcccac
      541 aggcgctctt ggatgacccg atcaatggcg tagggtgcgc cggggacgcg catcgccggt
      601 tcgacaagtg ctcgcactgt acaccaacgg ctccgatcat tggcgctaag aaatgggcgt
      661 cgcaagcggg cttcactttc taaggctttc cgtatgcgat caagctcttt ttccaaagag
      721 gccccggtga cgcctttggc cacgagctca gagcggcgga cttccaccct gcgctcgatt
      781 tcctcccagc gtcgccgctc cagttgatac acatcaaccg agtccacggc aatgatccgt
      841 cccaacatga ttcccgcgga aaccgctatc atcaaaaaaa ggacctgtcg ctgcacggga
      901 agcaaggacg tctgctggga atcaacggtc gatgttttca tcgcatctca cgcttcggga
      961 cgatgaggga ctcctggttc acatcatgtc ggataatgat ggcgaggtag gctaatcttc
     1021 ttccaggata attggccaga atgccaagcg aaaccctgga tatcgcgtat tgcccctttt
     1081 ggatcaagtc ggcgacgtgt ccaatcggtg ggataatgac acccggcgac cgacgattgc
     1141 gtcgggcagg accgccatcg taagactatc aggtgcttgg cgggccaccg ttagtgccga
     1201 tttcatcggc aagagaatat gtcttctcgt gttggatgtg ataggcggtg agcaactctg
     1261 ccaaaaagcc cacactcatc agttgggcgc ccaaaagaag taatcccaca gagtagatca
     1321 caagaggacg gtcgcggaga taaaccacgt ccatgccggg gatgatgcga gagatcgtcc
     1381 actcgagtgc gaggaaggtc agaattaggg cgctgacaag aaaggcaaca agcccaatgc
     1441 tccccaggaa gtgttgcggc cgggtgccaa atccggtgag gaagcgcacg gtcaatagat
     1501 cgagaaaacc cttgataaat cggccaaacc cgtacttgga ccgtccatat cggcgaggcc
     1561 gatggtggat gacgatttcg gacaccttaa atccccgatt tgccgccagg acgggg
//
LOCUS       DAOPTR010000978         1614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_59433_length_1614_cov_3.907633, whole genome shotgun sequence.
ACCESSION   DAOPTR010000978 DAOPTR010000000
VERSION     DAOPTR010000978.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59433_length_1614_cov_3.907633"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="PKI05_15070"
     CDS             complement(<1..743)
                     /locus_tag="PKI05_15070"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate-2-kinase family protein"
                     /protein_id="HOA53574.1"
                     /translation="MFSSALRNDALAIWHAGLDAVKADRLVAENICVEGSRLLIGKNA
                     DLVIELDRVDRLLVIGVGKAGAGMVRGLEKAIPDNLAAEKQLTGWVNVPEDCVGPTRW
                     IRLHPARPAGVNEPTESGIYGTERILELVRSAGPKDVCLCLLSGGGSALLPAPIEGIT
                     LQDKQKVTQVLSAAGANIAELNTVRKQLSKVKGGKLLRQCRAGWLITLIISDVLGDPL
                     EVIASGPTVPDPTTPGEALDVLHRYCGQQG"
     gene            complement(763..>1614)
                     /locus_tag="PKI05_15075"
     CDS             complement(763..>1614)
                     /locus_tag="PKI05_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylgalactosamine 6-sulfate sulfatase"
                     /protein_id="HOA53575.1"
                     /translation="IRWPKHIPAGLRISQIAAAIDLFPTLVSLAGIKANPLKPLDGWD
                     LSPLLLGKQTAWPDRLIFSHQAGRVSVRNQRYMLDAQGRLYDLQTDPGQTRDITKELP
                     DVAEKLQAAVNEFRATALPQTPDDRPFPVGYREFPVTFLPARDGVPHGNIRRSANAPN
                     CSFFTNWLSVDDAIIWDIEVHTAGTYEVCVFYTCREKDVGVELEVSFGSASVKGQVTE
                     AFDPPLRGQEFDRVPREGESYVKDFKPLLFGTLTLPAERGQLSIRATKIPGERAIDLR
                     MVTLTLK"
BASE COUNT          363 a          479 c          425 g          347 t
ORIGIN      
        1 ccttgttggc cgcagtaccg gtgcaaaaca tctaatgcct ctcccggtgt agtcgggtca
       61 ggcacggtag gcccggaggc gatcacttcc agcggatcac ccaacacgtc ggagataatc
      121 agcgtaatga gccagcccgc ccggcactgc cgcaacagtt tgcccccctt aaccttacta
      181 agctgttttc ggaccgtgtt caactcggcg atattggccc cggcagcact caacacctgg
      241 gtaacctttt gcttatcttg gagtgtgatt ccctcaatcg gggctggaag taaagccgat
      301 cctcctcccg atagaaggca aaggcaaaca tccttgggcc cagcagaacg gacgagttcc
      361 aagattcttt ccgttccata aatccccgac tcagtcggct catttacgcc ggccggtctt
      421 gccggatgaa ggcgtatcca gcgcgtaggc ccgacacagt cttcgggaac gttcacccag
      481 cccgtcagtt gtttttccgc cgccaggttg tcgggaatcg ctttttcgag gcctcgcacc
      541 atacctgccc cggccttccc gacgccaatg actagcagcc gatcgacacg atcaagctcg
      601 atgactagat cggcgttttt tccgatcagc aggcgacttc cttcgacaca aatgttctcg
      661 gcaaccaggc ggtctgcctt gacagcatcg aggccagcgt gccagatggc cagggcgtca
      721 tttcgcaacg cggatgaaaa catcgatcaa tccatctctt gatcacttca atgtgagcgt
      781 caccatccgc agatcaatgg cgcgttcacc agggattttc gtggcgcgga tcgagagttg
      841 accgcgctcg gctggtaatg taagagttcc aaataataag ggcttgaagt ccttcacata
      901 agactctccc tcacgcggca cgcggtcaaa ctcctggcca cggagaggtg gatcgaaggc
      961 ctcggtgacc tgccccttaa cgctagccga accaaaacta acctcaagtt ccacccccac
     1021 atctttctcg cgacaggtgt agaagacaca cacctcgtag gttccggccg tgtgaacctc
     1081 aatatcccaa atgattgcat cgtcaactga cagccagttc gtgaaaaacg aacaattagg
     1141 cgcgttcgca ctacggcgga tatttccatg aggtacgccg tcgcgggctg gcaaaaacgt
     1201 tacgggaaac tctcgatatc ccaccgggaa gggacgatca tccggggtct gaggcagggc
     1261 agttgcccga aactcgttga cagcagcttg tagtttctcc gcgacgtccg gtagctcctt
     1321 ggtgatatcg cgcgtttggc ccgggtcagt ttgcaaatcg tataggcggc cctgggcatc
     1381 taacatgtac cgttgattcc gaacactgac ccgacctgcc tggtgactga aaatgagacg
     1441 gtccggccaa gcggtctgct tacccaatag gagcggcgac aagtcccatc catccagcgg
     1501 ctttagagga ttcgccttga ttccggccaa ggagactaat gtcgggaata ggtcgatcgc
     1561 tgcggcaatt tgggaaattc gaagcccagc cgggatatgt ttcggccaac ggat
//
LOCUS       DAOPTR010000979         1612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_59546_length_1612_cov_2.871548, whole genome shotgun sequence.
ACCESSION   DAOPTR010000979 DAOPTR010000000
VERSION     DAOPTR010000979.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59546_length_1612_cov_2.871548"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PKI05_15080"
     CDS             <1..667
                     /locus_tag="PKI05_15080"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOA53576.1"
                     /translation="AAWWPLSHGLPVLHNVEIAAAGSRRKIGRTGFRRTSIDRGADGR
                     DFAISINGQRIFCRGACWTNADIVDLPGDRAAYEPLLRLAADLGMAASALLAWVVRLV
                     GSMVLDREALGFGDVTLMAMLGAYLGWQPGIILFFLAPFAGLVVAIIILILRRETEIP
                     YGPFLCLGALVTIVFWRPIWGFASRIFELGFILLLLAAACLVLLALLLLVIRFIRERL
                     FGV"
     gene            1103..1327
                     /locus_tag="PKI05_15085"
     CDS             1103..1327
                     /locus_tag="PKI05_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOA53577.1"
                     /translation="MAEGVIKKLVVDRGFGFIQTSGIRKSDVFFHFSAVEGARFEDLR
                     EGQSVEYELSSDAPNPGRGKGPRAVSVRPK"
BASE COUNT          301 a          402 c          497 g          412 t
ORIGIN      
        1 cgccgcatgg tggccgctca gccatggcct gccggttttg cacaatgtcg agatcgctgc
       61 tgcaggcagc cgccgcaaga tcgggcggac aggctttcgc cgcacctcga tcgaccgggg
      121 cgcggatggg cgcgacttcg ccatatcgat caacgggcag cgcatcttct gccggggcgc
      181 ctgctggacg aatgccgaca tcgtcgacct gcccggcgac agggctgcct atgagccgct
      241 tctgcggctg gcggcggatc tggggatggc ggccagcgcc ctcttggcat gggtcgtccg
      301 cctggtgggt tccatggtcc tggatcgtga ggccctcggc tttggcgatg tgacactcat
      361 ggcgatgctc ggggcgtatc ttggttggca gccggggatt attttatttt tcctggcccc
      421 tttcgccggg ttagtggtgg ccatcattat cctgatcttg cgacgagaaa cagaaatccc
      481 ctatgggccg tttttgtgcc tcggggcgct cgtaacgatc gtcttctggc gacctatctg
      541 gggatttgcc tcccgaatct ttgaattggg gtttatcctc ctcctgctcg cggcggcttg
      601 cctggttttg ctggccttgt tgctattggt gatccggttc attcgggaaa gactttttgg
      661 cgtgtagtct cattctgcga aaagtcgttg ctcaccatcg aagggtggag aaatgtgagc
      721 aaatttatgg gcgcgtttgg cgaatgcgaa ataaatcgtt gaaaagatgc tcactgaggc
      781 gaaaacaacg gggtgagcgg ttttgatgag caaaacccac aaaagagggg ttcggaaaaa
      841 agtgaaatat tccgataaaa gggaaagatg cctcttgcct gaaggctgtc tttaggctag
      901 agttttagtt gtcgtgagaa tggaccggtg ggccccaaga tcgatccggg acattctctg
      961 ggagttcgga tggttttgtg ttagctgagc cactccttgg tctaacggcc aacgagcggg
     1021 ttgctcacga catgagacag tccgagttgt tttgtgtgtg cggaggtaac tccgctcgtt
     1081 acgtttttga ggagtggctt taatggccga gggtgttatt aagaagttgg tcgtggatcg
     1141 tggttttggt ttcatccaaa ccagcggcat ccggaagtcc gatgtgtttt tccacttttc
     1201 cgcagtggaa ggtgcccgct ttgaagacct tcgcgaaggt caaagcgttg agtacgagtt
     1261 aagctcggac gcaccgaatc cgggtcgggg aaaaggcccc cgggcagtct ccgtgcgacc
     1321 taagtagtcc ttagtctccg tgtgagacgc cgtgaagctc atggctggct tcacatcttt
     1381 ttcggctgcc ccgtcgcatc cgctcgtgta aactcaggat cagttgggca gatgacatct
     1441 ggcttgacgt cgagcagtaa gctggagttt tatcgtcgat agatgacgaa aacagggagc
     1501 aagggctccc tgtttttttt gcgcgccggt ttctcgaaag gcttccctca catcggtacg
     1561 aactccggga cgaccggttc cgcaatgact cctgactcgt aagccttttg ca
//
LOCUS       DAOPTR010000980         1609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_59734_length_1609_cov_2.240669, whole genome shotgun sequence.
ACCESSION   DAOPTR010000980 DAOPTR010000000
VERSION     DAOPTR010000980.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59734_length_1609_cov_2.240669"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="PKI05_15090"
     CDS             complement(<1..917)
                     /locus_tag="PKI05_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53578.1"
                     /translation="MKIRPLCLGVLAWCLWSVAGRSEDKLPVVIWPVDVHVKVLRDAQ
                     PPEDIQLPLVVKAAQNEYESAQVAVRTAREIKRLTIRASEFRNPENGAILPAESVRMR
                     FVGYIPLKANTPNAERIRVAAAPCEIPDPLLDETEISLAAETSQPIWVTFWIPPGTVP
                     GVYHGELAIVADGNQAKIPVELHVFPFALPDERHLFVTNWFSLGNIAKAHQVEIWSDA
                     YWDILAKYAQNMGQHRQNVVLTPWTLIEVTRNPDGQLTFDYSRFDRFVELFEKMVVAD
                     LIEISHVGHPKGGWGGPVELSNLRAKDKST"
     gene            1369..>1609
                     /locus_tag="PKI05_15095"
     CDS             1369..>1609
                     /locus_tag="PKI05_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53579.1"
                     /translation="MTKHGSSRGIRSQVYWLIWAFCVLIPVLAGCNSGPSQEQPKGGE
                     QPAASNSSQNLPQVSQSELQKVTGDPVSAVKVFLEA"
BASE COUNT          363 a          426 c          424 g          396 t
ORIGIN      
        1 gtcgatttgt cctttgcccg gaggttcgac aattctacag gtccacccca acctcccttg
       61 gggtgcccca catgggaaat ctcgatgaga tctgccacca ccattttctc aaacagttcc
      121 acgaaccgat caaagcgaga atagtcaaat gtcaattgtc catcaggatt ccttgttacc
      181 tcaatcaagg tccaaggtgt aagaaccaca ttttggcggt gttggcccat gttctgggcg
      241 tacttcgcta ggatatccca ataggcatcg gaccatattt cgacctgatg ggccttggcg
      301 atatttccca ggctgaacca attggtcacg aacagatgac gctcgtcagg cagagcgaaa
      361 gggaaaacat gcaactcgac tgggatcttt gcttggttgc cgtccgcaac aatcgccaac
      421 tccccgtgat agacccctgg gacagttccc ggagggatcc aaaacgtcac ccaaattggt
      481 tggcttgtct cggctgctag gctgatctcg gtctcgtcca aaagcgggtc ggggatttcg
      541 caaggcgcgg ctgccacacg gattcgctcg gcatttggcg tgtttgcctt gagtggaata
      601 tagccgacga accgcatgcg aacactctcg gccggcagga tcgctccgtt ttctggattg
      661 cgaaattcag acgcccgaat tgtcagccgt ttgatttctc tcgcggttcg cacagcgact
      721 tgggcagatt catattcgtt ttgggctgcc ttcacaacta acggaagttg aatatcctct
      781 ggcggctggg catcacgaag cactttgaca tggacatcaa ccggccaaat gacaacgggg
      841 agtttgtcct ccgatcgccc ggcaacactc cacaaacacc aggcgagaac gccgaggcaa
      901 aggggtcgta tcttcatggc ttgtccctcc tacgcgcagg ttactttgaa aatgtgttcc
      961 gataggatct tgggtactac atcactttga cgacagccca ttgtagcaat gtaatcggtg
     1021 gtggacatcg gactttcccg atttggaccg gcccactcct ccgttcacgc gagcttccgg
     1081 tctgtaccaa gcttgtgcgc gctgtctgtg gtgaaagcgg gaccctcagt ttcatttcgc
     1141 cttgacgggc ccgtcaaaaa ttggcaaact gcctgttgtt aggcgttggc ccgcaaggca
     1201 cccgttatcc gcggggtcac gaaggaaatg gtctgtcccg caaagagtgc aatttctatg
     1261 gggttaatgt cagattcgcc ctacagctcg gtgcgataag cagagggctg tattctgcct
     1321 tcctaaggca tgcaagcacg ttatctagca agtgacaagg aggtttttat gacaaaacac
     1381 ggaagtagcc ggggcatccg atcacaggtt tattggctta tttgggcatt ctgtgtgctc
     1441 attcctgtgt tggcaggatg taatagtggc ccatcccagg agcaaccaaa aggtggggaa
     1501 cagccggccg ctagtaattc ttcccaaaac ctgccgcagg tttctcaatc tgaattgcag
     1561 aaagtcactg gtgatcctgt atccgcagta aaggtttttc ttgaggcgg
//
LOCUS       DAOPTR010000981         1607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_59840_length_1607_cov_2.604381, whole genome shotgun sequence.
ACCESSION   DAOPTR010000981 DAOPTR010000000
VERSION     DAOPTR010000981.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59840_length_1607_cov_2.604381"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PKI05_15100"
     CDS             complement(<1..289)
                     /locus_tag="PKI05_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53580.1"
                     /translation="MGRHLFLSVIIAVLGILICSCSPPPQFRLNSEGRDPATISPAQR
                     EAISSLLTDFFGTPDQPTVPRGVTLSLDDITKAAGPVGRDLRKVERGLYRKY"
     gene            complement(529..1194)
                     /locus_tag="PKI05_15105"
     CDS             complement(529..1194)
                     /locus_tag="PKI05_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53581.1"
                     /translation="MINKFRSTRRAFLKSSLSLAVGTAAVGLTRKATFGITESARGEV
                     RGRFVYDGQPPERKKLKVDKDVDCCGKYDIRDESLMVGLENGLANVFVYVRTRGIPIP
                     PDMAEQIPKRVVLDNHECIFKPHCMAIWYPVQEFYIVNSDPIAQNVAFSPLGDLPANI
                     ILAPAPGDNIDAVWRFRRSQTEPVLIVCNYHPWESAYILPRDNPFFAISDLHGNFVIS
                     MCQ"
     gene            complement(1421..>1607)
                     /locus_tag="PKI05_15110"
     CDS             complement(1421..>1607)
                     /locus_tag="PKI05_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53582.1"
                     /translation="KAIRPYTAMPVNFPENGIPLDRSVFDADSRVQLEAVVDLLQNYD
                     WYLKQRVRQELGPAQSQ"
BASE COUNT          392 a          453 c          385 g          377 t
ORIGIN      
        1 aatattttcg gtaaagccct cgctccacct ttctaaggtc acggcccaca ggccctgcag
       61 ccttcgttat gtcatccaat gacaacgtca ccccccgcgg tacggttggc tggtctggtg
      121 taccgaaaaa atccgtcagc agcgacgaga tggcttctcg ctgggccggt gaaatcgttg
      181 ccggatctcg cccctcgcta ttcagtcgaa actgtggggg aggcgagcag gagcatatga
      241 gtatccccaa tacagcgatg atcacggata agaacagatg tctccccata tctaatatcc
      301 aggttagcct ccgtcatgtg cgtgcataca tttaccagcc acgtcaaacg ccgccaccaa
      361 gtcttgggag gcattctcct caaacgacat tcaactgctc tggcctgacc ttgatcacac
      421 ccaggtcatt tactcctggt ttgacgttaa ccaccaggcg accacggggg gcctgtggta
      481 aatccagttg acggacccgc tcttgccaaa tttgaaattc ccactcgcct actggcacat
      541 tgagatgaca aagttaccgt gcaaatcgct aatcgcaaaa aatgggttat cccggggtaa
      601 aatgtaggca ctttcccagg gatgataatt gcaaacaata agcacaggct cggtctgact
      661 tcggcggaac ctccagactg catcgatatt atcacccggc gcgggggcaa ggatgatgtt
      721 agccggcagg tctcccaacg ggctaaacgc cacattctgg gcaatcgggt ccgagttgac
      781 aatataaaac tcctggacag ggtaccaaat ggccatgcaa tgcggtttga aaatgcactc
      841 gtggttgtcg agcactaccc tcttcggtat ttgttcagcc atatccggcg ggatggggat
      901 cccccttgtt cgaacataca caaacacatt cgccaaccca ttttccagac cgaccatcag
      961 ggactcatca cggatgtcat actttccgca acagtccaca tctttatcga ctttcagctt
     1021 ttttcgctcg ggcggttggc catcatagac aaaccggcca cggacctcgc cacgagcgga
     1081 ctcagttatc ccgaaagtgg cttttcgcgt taaccctacg gcggctgtcc cgacagccag
     1141 tgacaaactg ctcttaagaa atgcccgacg ggtgcttcga aatttgttta tcatctttgc
     1201 ttctctgata cgacattagt acgatctcat cgcaagtgag ggaatcacca aacaaacaat
     1261 cttttttctt gcccggtagg caacaaatcg gtgggaatca gcatgaggat agcaggactt
     1321 cccgtgtgca gtccattgta cataattgtc cgactgaaaa gcacatcgcg tctgtttttc
     1381 gacccacaaa gcaatcctgg gaaattcaag ccgccgctgt tcactgagat tgcgcgggac
     1441 ccaactcctg cctcacacgt tgtttgaggt accaatcgta gttctggagg aggtccacca
     1501 cagcttccag ttgaacacga ctgtcagcat cgaagacgct tcgatccagc gggataccgt
     1561 tttcgggaaa gttgaccggc atggccgtgt agggccgaat tgccttc
//
LOCUS       DAOPTR010000982         1606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_59880_length_1606_cov_3.548678, whole genome shotgun sequence.
ACCESSION   DAOPTR010000982 DAOPTR010000000
VERSION     DAOPTR010000982.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59880_length_1606_cov_3.548678"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            300..>1606
                     /locus_tag="PKI05_15115"
     CDS             300..>1606
                     /locus_tag="PKI05_15115"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HOA53583.1"
                     /translation="MALSRISSIPLFLLTVCLPGAWAFTEPLATRVGTAEVQVSDAQG
                     RRLFYLDPAHLEALGQTGGRIVGGPPQVVSDSENLKGLRFDGNTWIVFQEASHLDVPQ
                     LTIEICFKGQFSAPARYNPCLIAQRESGDHRQTRWSVHIMGDYSAIALWNGKSVAVFR
                     SPAGALKPEVWYHLVIVTQKSGTTVYLDGVPCITDSPSWTINIQEKGRPLTLAASQPS
                     GHEAFLGALANVVIYDTAWTAEQVAARTDELGFRDERLALAQQYQEILKRQAEEQARL
                     ADLREKRRMELMNDPALVTRGQPTVYEAERLECIDIPIGGIGVGAIHMNGRAERHAWQ
                     IFGSVAYRQIPESFFAIGVSTPTRSIVRTLQTTSVAGIPPMQRLRLEAPYPFAVYRFQ
                     DEEVSCHVTLTAFNPLIPLHARDSAIPCAVYRIGIHNPMNEPVT"
BASE COUNT          334 a          446 c          449 g          377 t
ORIGIN      
        1 ccccaaccct cggttccgaa gaccgatgct ctatccaatt gagctacggg cgcacaaacc
       61 gcatcagcag cgacccagtt ctgctggact gtgcccacca cgactacggt cgcttcttat
      121 tactatgcta cccgcccggc agtagcgcgc gaagggtacg cgccgcaatc gtgatcgttt
      181 tttccatacg cttgatgcaa gcaaacgtgg gcggtaccat aaactcggcc atgtggattg
      241 gacctcgtga ctgacctacc cagtcctctc cggagttgca tccatttgga gacagtttga
      301 tggcgttatc acgaatctcg agcatcccgc ttttcctgct tacggtctgt ttaccaggag
      361 cttgggcttt cacggaacca ttagccacac gggtcggaac agccgaggtg caagtttcgg
      421 acgctcaggg tcgccggttg ttttatcttg atccggcgca tttggaggcc cttggacaga
      481 cgggcgggcg aatcgttggg ggaccgccac aagttgtctc cgattctgag aatttgaaag
      541 gcctccgttt tgacgggaac acctggatcg tttttcaaga ggcgtctcac ctggacgtac
      601 cgcagctgac cattgagatt tgttttaagg gccagttctc ggcaccggcg aggtacaatc
      661 cgtgcttgat tgctcaacga gaatccggtg atcaccggca aacgcggtgg agtgttcaca
      721 tcatgggcga ctattcggca attgccttgt ggaatggcaa gagcgttgcc gtgtttcgat
      781 cacctgcggg ggcactcaaa cccgaagtgt ggtatcatct ggtcatcgtg acgcaaaaat
      841 ccggcacaac cgtttatctc gatggggtcc cgtgcatcac ggactcgcct tcttggacga
      901 tcaatatcca ggaaaaggga cgtccgctca cgcttgctgc gtctcagcct tccggacatg
      961 aggccttctt aggggctttg gccaatgttg tcatttatga tactgcgtgg accgcggaac
     1021 aagtggcagc acgaaccgat gagctcggct tccgggatga gcggttggcc ctcgcgcaac
     1081 aatatcagga aatcctcaaa agacaggcag aggagcaggc ccgacttgcg gacttgcgtg
     1141 aaaagcggcg gatggaattg atgaacgatc cagcacttgt cacgcgggga cagccgaccg
     1201 tttatgaggc ggaacgtctg gaatgcattg acattcctat cggtggtatt ggcgtgggtg
     1261 ctattcacat gaatggccgc gccgaacgtc acgcttggca gatttttggt agcgtggcct
     1321 atcgccaaat cccggagagc ttcttcgcca tcggtgtatc aacacccacg cgatcgattg
     1381 tgcggactct tcagaccacg tccgtggccg ggattccgcc gatgcaacgg ctgcgtttgg
     1441 aagcccccta tccgtttgct gtttatcgtt tccaggacga agaggtatcg tgccatgtaa
     1501 ccctgacagc tttcaacccg ctcattccgt tgcatgcccg ggattcagcc attccctgcg
     1561 ccgtgtatcg gattggtatc cacaatccga tgaacgaacc ggtcac
//
LOCUS       DAOPTR010000983         1605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_59928_length_1605_cov_2.954194, whole genome shotgun sequence.
ACCESSION   DAOPTR010000983 DAOPTR010000000
VERSION     DAOPTR010000983.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59928_length_1605_cov_2.954194"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1394
                     /locus_tag="PKI05_15120"
     CDS             <1..1394
                     /locus_tag="PKI05_15120"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOA53584.1"
                     /translation="TKLILYYRIVSFKVTAFLLRMAGSARETAQDRFHGFRQAGSGKI
                     APVVPQPESQFIHRFDNGLVLLGESMPWLDSVGLALAVPAGSAFDPEDRAGLASLTTE
                     MVLRGAGSRDSRQFLEDLENLGIIWSEAVSRLHIVLTATMRASQLSEALPIFADVLLR
                     PRLPREELEPARQGLIQEIRADRDLPEVKLRRELRRRCFPYPWNRPTQGEEETVAAIE
                     WEDVRDFVDQHFRPDGSIIAVAGRFDWDETKALVERLFRDWTGVAPSPLPWRWERERV
                     KHVPYDSQQTHIGVAYKSVPYDHPDYFLARASLGVLSGGTSARLFMEVREKRGLCYTV
                     QATYHVIKNMAAVFCYSGTTAERAQETLDVLLGEISRLKEGVQSEELERVKVQIKSSL
                     VFEQESSASRARSLIGDWVLLGRVRPVTELKQSIDALTVHAVNRFLAENPPDDFTIVT
                     LGPHALEVPREVL"
     gene            1381..>1605
                     /locus_tag="PKI05_15125"
     CDS             1381..>1605
                     /locus_tag="PKI05_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOA53585.1"
                     /translation="MRFYSHILPNGLEVIAECSPSAFSTALGFFVRVGARDEPEDISG
                     VSHFLEHMAFKGSGSRTADDINREFDEIGAV"
BASE COUNT          336 a          407 c          482 g          380 t
ORIGIN      
        1 ccacaaaact catcttatat tatagaattg tttctttcaa agtgacagcg ttcctcttgc
       61 gcatggccgg gtcagcacgc gaaactgcgc aagatcgttt ccacggattt cgtcaagcag
      121 gttcagggaa aattgctcct gtggtaccac agccagaatc acagtttatc cataggtttg
      181 ataatgggct tgtccttctt ggggagagca tgccctggct ggattcggtg gggctcgcgc
      241 tggccgttcc ggcagggagc gccttcgatc cggaggatcg cgccggcctg gcctcattga
      301 caaccgagat ggtcctacgg ggggcaggca gccgtgattc acggcaattc cttgaggacc
      361 tcgagaactt ggggatcatc tggtcggaag cggtcagccg ccttcacatc gtattgacgg
      421 ccaccatgcg ggccagccag ttgtcggaag cccttcccat atttgccgat gttcttttgc
      481 gaccgcgtct ccctcgggag gaattggaac cggctcggca aggactgatc caggaaattc
      541 gggcggacag ggacctgcca gaagtcaaac tccggcgaga attgcgacga cgatgttttc
      601 cctatccctg gaatcgaccc acacaaggtg aggaggagac ggtcgccgcc atcgagtggg
      661 aggatgtgcg agattttgtc gatcaacact ttcgtcccga tggctcgatc atagctgttg
      721 ccggtcggtt cgattgggac gaaacgaagg cccttgtgga aaggttattc cgcgattgga
      781 ctggcgtagc tccgtcaccc ctgccctggc gatgggagcg ggagcgagtc aaacatgtcc
      841 cctacgattc gcagcagacg cacattggtg tggcatataa gtctgttccc tacgatcacc
      901 cggattactt tttggcgagg gcttcacttg gtgtgttatc tgggggaaca agtgcacgtc
      961 tctttatgga ggtgcgagaa aagcgcggat tgtgttatac ggttcaagcc acgtaccatg
     1021 tcatcaagaa catggcggcg gtcttctgtt actcggggac cacggcggag cgcgctcagg
     1081 aaacgcttga cgtccttctt ggcgagatct cccgtttgaa agagggtgtg caatcggagg
     1141 aactcgagag agttaaggtc caaattaaaa gcagtctggt attcgagcag gaatccagtg
     1201 cctcacgagc gcgatctctg attggcgact gggtactgct gggtcgggta cgacctgtga
     1261 cggaactgaa gcaatcaatt gatgccttga ctgtgcacgc tgtcaatcga ttcctggccg
     1321 agaatccgcc tgacgatttt acgatagtca cattgggacc gcacgcattg gaggtacctc
     1381 gtgaggtttt atagccatat cctcccgaat gggctcgagg ttatcgccga atgttcgcct
     1441 tctgcctttt cgacggcgtt gggattcttt gtgcgggtgg gggctcgcga tgagccggaa
     1501 gatatctccg gcgtaagcca tttccttgaa cacatggcgt tcaaaggctc ggggagccgc
     1561 accgctgacg atatcaatcg tgagtttgac gaaatcggtg cggtg
//
LOCUS       DAOPTR010000984         1594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_60516_length_1594_cov_3.519818, whole genome shotgun sequence.
ACCESSION   DAOPTR010000984 DAOPTR010000000
VERSION     DAOPTR010000984.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60516_length_1594_cov_3.519818"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..767)
                     /gene="rplC"
                     /locus_tag="PKI05_15130"
     CDS             complement(78..767)
                     /gene="rplC"
                     /locus_tag="PKI05_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011121602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOA53586.1"
                     /translation="MAIGLLGRKVGMTQIFDDAGNVIPVTVIQAGPCHILQIRTPQRD
                     GYEAIQLGYLDKPRHRANRAERGHVARLESKRQKLLAAAGIQAPPKANCEPKRFIKEF
                     RTSIEGFSVGQVLTVDLFKDVAAVDVTGTTKGRGFTGVMKRHGFSGQRASHGVKNVHR
                     HPGSTGCNTSPGRVVPGRRMAGHYGHEKCTIRNLKLVRIDSERHLLLVKGAVPGPVGG
                     LVIIRPTNKVG"
     gene            complement(928..1266)
                     /gene="rpsJ"
                     /locus_tag="PKI05_15135"
     CDS             complement(928..1266)
                     /gene="rpsJ"
                     /locus_tag="PKI05_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOA53587.1"
                     /translation="MSATINEIIRIRMESYDHAVLDQSAREIVDTVKRTHSKVHGPIP
                     LPTRIERYTVLSSPHVDKKARQQFEIRTHKRLIDILEVTPATIEALNKLTLPAGVDIK
                     IKATSRGRSR"
     gene            complement(1341..>1594)
                     /locus_tag="PKI05_15140"
     CDS             complement(1341..>1594)
                     /locus_tag="PKI05_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53588.1"
                     /translation="DAVAFATPSLAAGFSPKFDSRFCVPGGLAKVAGRCFNTGTQCLK
                     ALRDRVTLLCFPTCDRDNPFRSERRARRQVPIEMPGRSM"
BASE COUNT          330 a          438 c          433 g          393 t
ORIGIN      
        1 atgatacacc tgcttctttg tgggacctaa cgtcatttct ctcgtttttg gtcctacaga
       61 atgttgcttc ccctcgctta ccccaccttg tttgtcggcc gaataatgac caatccgcct
      121 accggtccgg gaactgcacc cttgaccagg aggaggtgac gctctgaatc aatccgcaca
      181 agtttcagat tgcgaattgt gcatttttcg tgtccgtagt ggcctgccat tcgccgacca
      241 gggacgacgc gaccgggaga agtgttacag ccggtagaac cagggtggcg atggacgttc
      301 ttaacaccgt ggcttgctcg ctggccggaa aagccgtgcc gtttcatcac acctgtaaag
      361 ccgcgaccct tggtcgtgcc ggtcacgtcc actgcggcaa catccttgaa aagatcgact
      421 gtcagaacct gccctacgga gaaaccttcg atggaagtcc ggaactcttt gatgaaacgc
      481 ttcggctcgc agttggcttt gggaggagct tggatgccgg cagcggcgag gagcttttga
      541 cgtttgcttt ctaacctggc cacatgcccg cgctctgccc gatttgctcg atggcggggc
      601 ttgtcaaggt agcctagctg gatggcttcg tacccgtcgc gttgcggggt tctgatctgc
      661 aaaatgtggc agggacccgc ttggatcacg gttacgggga tcacgttccc tgcatcatca
      721 aaaatttgcg tcatgccgac cttgcggcca agtaatccga tcgccattgt ttatctacct
      781 tatctacatt gccctcgagg atcgtgggat tggtcccaga ttacctctgg ggcttccagt
      841 ccatacacgc gatggatgtt tatcgtctgg ttctggcctt tggtttcagc gttgaaaaca
      901 acccaacact gcacaaggtt ttaggtttta gcgacttcgc ccccggctgg ttgccttgat
      961 cttgatgtct acaccggccg ggagtgtcaa tttgtttagc gcttcaatgg ttgcaggcgt
     1021 aacctcaagg atatcgatca accgcttgtg tgtgcggatt tcgaactgtt gcctcgcctt
     1081 cttgtccaca tggggacttg agaggacggt atatcgctca attcgagtgg gcaaagggat
     1141 tgggccgtgg accttggaat gggtccgctt aaccgtatcg acgatctcgc gggcactctg
     1201 gtctaagacc gcgtgatcgt aggattccat ccgaattcga ataatttcgt taatggttgc
     1261 cgacataaat ctgctatcct cgccacgccc ggcgacaccg actggccaac tatttccgca
     1321 aaaggtgaaa aacctcgatt ctacatgctt cggccaggca tctcaatggg gacctggcgt
     1381 cgcgctcggc gctcgctgcg gaaagggtta tcccgatcac aggtgggaaa gcacagaagc
     1441 gtcacacgat cgcgaagcgc ctttaagcac tgggttcccg tattaaagca tctaccggcc
     1501 acttttgcca agccccccgg aacgcaaaaa cgggaatcga acttcggaga aaagcccgcg
     1561 gctaaagacg gtgtggcaaa ggctacggcg tctc
//
LOCUS       DAOPTR010000985         1587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_60878_length_1587_cov_3.776762, whole genome shotgun sequence.
ACCESSION   DAOPTR010000985 DAOPTR010000000
VERSION     DAOPTR010000985.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60878_length_1587_cov_3.776762"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            432..>1587
                     /locus_tag="PKI05_15145"
     CDS             432..>1587
                     /locus_tag="PKI05_15145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOA53589.1"
                     /translation="MNERTRPGRLEATDCRTAIARRNLPVLRVDKLPITSLAFRFSSF
                     IFLILLTAPGCSTSLREWWANGFKVGPNYCPPSAEVGEAWIDSDQPGVKTSGDVTYAH
                     WWTVFNDPTLNELVYTAYLENLPLQIAAWRIMEARYQLGVARGYLWPQEQQAMGAFSR
                     NQISKEGFPMSKFSEIPGFKMSYDDWVAGFSLAWELDFWGRFRRTIESAEAHVQAQEE
                     DYNAALVLLQAEVASTYIQLREIDERLELARKNVALQKDTLAIAKARFEQGVVSQLDV
                     HQAQAILSNTESMIPMLEAMRRVSENRLCTLLGQPPQDLDPILGPSGKVPDVPPEVVV
                     GIPADLLRRRPDVRRAERLAAAQCARIGIAEAEFYPHIALTGQLAYQAENF"
BASE COUNT          363 a          455 c          408 g          361 t
ORIGIN      
        1 aagccgtaca aaaactccct cgcaaactca gacaactcat attcgaccaa ctctgtcaag
       61 ccacgtaggc acccaagact ggcaaaagtc gagctaagtt aacaccgtga agatcggtcc
      121 actaacgtgt atcggcgccg acacccaaga cccaatggtt cgattctctg acgttactac
      181 gcaaagtttg gacgatcgtt catatatcta tgatcatagt acatcttgtt gaccgctaag
      241 tcaatcgtgc gaccgtcctg ttttctggtt aactgtccag cccggattta ccgaagttac
      301 tcaatgcaac tctatgcgcg gttttttggg aaacatcgat tgtgtgatca ctgccgattc
      361 gtccgccggg cgaacctaac taaccaattt gcccagtttc ccgttcgtct tgatccacag
      421 ggggaatcgt catgaatgaa aggacgcggc cgggccgtct ggaagctacc gattgccgaa
      481 cggctatagc gagaaggaat ttacccgtct taagggtgga taaactcccc attacttcgc
      541 tagcgttcag gtttagctcg ttcatcttcc tcatcttgct tacagcgcca ggatgctcga
      601 ccagtcttcg cgagtggtgg gctaatggct tcaaagttgg accgaactat tgccccccct
      661 ccgcagaggt gggcgaggcc tggatcgaca gtgaccaacc gggggtgaaa accagtgggg
      721 acgtcaccta cgcccactgg tggacggtgt ttaacgaccc cacgctgaac gagcttgttt
      781 acacggccta tctggagaat ctgccgctcc agatcgcagc ctggcggatc atggaggccc
      841 gctaccagct cggtgttgcc cgaggctacc tctggcctca ggagcaacag gcgatgggag
      901 ccttctcccg taaccaaata agcaaagaag gcttccctat gtcaaaattt tctgaaattc
      961 ccggttttaa gatgtcttac gacgactggg ttgcgggatt cagtttggct tgggaactgg
     1021 atttttgggg gcgattccgc cgcacgatcg aatcggccga agcccacgtt caggctcagg
     1081 aagaagacta taacgcggct ctcgtcctcc tccaggcaga agtggcttcg acctatatcc
     1141 aactgcgcga gattgacgag cgtttagagc ttgcccgcaa aaacgtggcc ctccaaaaag
     1201 acacgttggc tatcgccaaa gcccgctttg aacagggtgt tgtcagccaa cttgatgtcc
     1261 atcaggctca ggctattctc tccaacacag aatcgatgat ccccatgttg gaggcaatgc
     1321 gccgggtctc ggaaaaccgg ctctgcacgt tgcttggcca acctccgcag gacttggatc
     1381 ccatcttagg gccatctggc aaagtcccgg acgtaccgcc ggaagtggtc gtaggtattc
     1441 cggctgactt gctccgtcgc cgcccggatg tacgaagggc agagcgtctg gccgccgccc
     1501 aatgtgcccg catcggcatt gccgaagccg aattctatcc gcacattgct ctcactggcc
     1561 aactcgccta tcaggctgaa aacttca
//
LOCUS       DAOPTR010000986         1584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_61051_length_1584_cov_2.824722, whole genome shotgun sequence.
ACCESSION   DAOPTR010000986 DAOPTR010000000
VERSION     DAOPTR010000986.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61051_length_1584_cov_2.824722"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PKI05_15150"
     CDS             complement(<1..381)
                     /locus_tag="PKI05_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53590.1"
                     /translation="MIRHVVFSLILAAACASLSLAEVVEFTSPGFPPSRMDAEGRLTE
                     DWGSLKIIVEGDGVMAQGGTVQVVRLEELVPAAVWSCEAGPIRVTATAYRAPVFPSGM
                     DVLTVTLQETAGQDRHVTLKMVVNP"
     gene            complement(378..>1584)
                     /locus_tag="PKI05_15155"
     CDS             complement(378..>1584)
                     /locus_tag="PKI05_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53591.1"
                     /translation="ASAKRLGITVEQQAQPLNSDASVDSWIEKLKAEKPHGVLVMLQH
                     LHAWNWANRIADETKLPTIMFAPVGTAFTGHLAGPSRKTGVFVISSLDWSAVEMGLRM
                     IRAKRMFEETRVLWIRGNQRNETVVERLGTKVLAIPRDTFNQEFDKQPVTDEIRDLAA
                     DLRKHARKIVEPNEEDTINAMRAYATAKRLLARENAHALSMDCLGMVGAKLVPTPPCG
                     AWTLLQDAGITAGCEADLYGAMSLMTTSYLLDRPGYMNDPVPETVHNTLIAAHCTSGT
                     RIAGFDQPPADYVLRNHSESALGVAMQVLWPEGQPITLVRFTGPNEMIIDTGKVVGNV
                     QTPPAGGCRTSVEIELDGIEDSRDTLGFHQVVTLGSHRRVLEGFCQLYGIKVTHSPRF
                     STFQYGGQA"
BASE COUNT          312 a          465 c          468 g          339 t
ORIGIN      
        1 cggattgact accatcttca gcgttacgtg acgatcctgg cccgcggtct cctgtaaggt
       61 aacggtcagc acgtccatcc ccgatggaaa caccggtgcg cgataggcgg tggccgtgac
      121 gcggatgggg cccgcttcac aggaccatac ggcagcagga acaagttcct ccagtcgcac
      181 gacttgaacc gtcccgcctt gggccatgac cccatcgcct tcaacgatga tcttgaggct
      241 tccccagtct tccgtgagcc ttccctcggc atccatccgt gagggtggaa agcccggaga
      301 ggtaaactcc acaacttcgg cgagcgatag gctcgcgcaa gcggcagcca aaatcagcga
      361 gaacaccacg tgacggatca tgcttggcct ccgtattgga aggtggaaaa ccgtggggaa
      421 tgcgtgacct tgatgccgta gagttggcag aatccctcca ggacgcggcg atggctgccg
      481 agggtcacga cctggtggaa acctagcgta tcccgcgaat cttcgattcc gtccagttcg
      541 atttccacgg aggttcggca cccaccggcg ggcggtgttt ggacattgcc tacgaccttg
      601 ccggtgtcga tgatcatctc atttggtccg gtgaagcgaa ccagcgtgat gggctggccc
      661 tcgggccaaa ggacctgcat ggccacgcca agggccgact ccgaatggtt acggagaaca
      721 taatcggccg gcggttggtc aaacccagcg atccgggttc ccgacgtgca gtgggccgca
      781 atcagggtgt tgtgtacggt ctcgggcaca ggatcattca tgtagccggg acgatcgagg
      841 agatagctgg tcgtcatcag agacatggcg ccatacaggt cggcttcgca ccctgctgtg
      901 atgccggcgt cttggaggag cgtccacgcc ccacaaggcg gtgtgggcac tagctttgcg
      961 cccaccatgc caaggcaatc catgctcaaa gcgtgggcat tttccctggc caaaagcctc
     1021 ttggctgtgg cgtatgcccg catcgcgttg atcgtatcct cttcgttggg ttccacaatc
     1081 tttcgtgcgt gctttcgcag gtccgcggcg agatcgcgaa tttcgtcggt gacgggctgt
     1141 ttgtcgaact cttgattaaa cgtatctcgg ggaatggcga gaacctttgt cccaaggcgt
     1201 tctacgacgg tctcgtttcg ctgattcccg cgaatccaca ggactcgcgt ctcctcaaac
     1261 atgcgtttag cccggatcat ccgcaatccc atttcgacag ccgaccagtc gagcgaggag
     1321 atgacgaaaa cacccgtctt ccgacttggg cctgccaaat gtcccgtaaa ggccgtgccc
     1381 acgggggcaa acatgatcgt gggtagcttg gtttcatcgg caatacggtt ggcccaattc
     1441 caggcgtgca ggtgttgtag catgacaagc acaccgtgcg gcttttccgc cttaagtttt
     1501 tcaatccaac tatcaacgct ggcgtcgcta ttcaacggct gggcctgctg ttcgacggtg
     1561 atgcccagac gtttggccga tgcg
//
LOCUS       DAOPTR010000987         1584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_61065_length_1584_cov_2.338784, whole genome shotgun sequence.
ACCESSION   DAOPTR010000987 DAOPTR010000000
VERSION     DAOPTR010000987.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61065_length_1584_cov_2.338784"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /locus_tag="PKI05_15160"
     CDS             complement(<1..1406)
                     /locus_tag="PKI05_15160"
                     /inference="COORDINATES: protein motif:HMM:NF019269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1598 domain-containing protein"
                     /protein_id="HOA53592.1"
                     /translation="MAPTYLRGETRLSDWETLMVSSWGRFGWMKSLAAVAAFAVLIVG
                     ASHGIAQTTGVYANRAVGGVMIDPSGFIQKAPSGSLAEIRQLWQSYHKELTGKLAQPV
                     ESRAISLRGLESQLAAAIQAGQPVPDEVACLGGLTRIEYVLVYPDKNDIVLVGPAEPW
                     KLHATGYPVGIKSGKPVILLDDLIVAMRSAFGPQRSVISCSIDPTAEGIRKARSFARS
                     RQGGVDPARFSAALEQLLGNQVVTVTGVPENTHFARVLVAADFRMKQISIGLEPAPIA
                     GLPSFPTLVSSSGRVPENTMPRWWLAPDYEPLLRDETGLTWKFQKASVKTMVEADLRG
                     ATGEREKIVEAAPVYRKWAELMTARYEELSKADPVFGQVRNCMDLATVAALIASEGLL
                     EKADLALPTILGNDGVKPAELTPAKYVPSKSVFGRVGRNWMFVTGGVEINAWELASRQ
                     SVSAELATWRQQLPTPST"
BASE COUNT          350 a          487 c          411 g          336 t
ORIGIN      
        1 tcggtcgatg gggtcggaag ctgctgccgc caggttgcca actccgccga gacagactgc
       61 cgagaggcca actcccaggc attgatctcc acaccaccgg tgacgaacat ccaattgcgg
      121 ccaacgcgac cgaagacgga tttcgacggg acatactttg caggggtcaa ttctgctggt
      181 tttaccccat cgttccctag gattgtcggc aaagccaggt ctgccttttc gaggaggcct
      241 tcgctggcga tcagggcagc caccgttgcc aagtccatgc agttccgaac ctggccgaat
      301 acgggatcgg cctttgacag ttcctcatac cgggccgtca ttagctcggc ccattttcga
      361 tagaccggag ctgcctcgac gatcttttcc ctttcacccg tcgcccctcg cagatcagcc
      421 tcgaccatcg tcttgacact cgccttttgg aacttccaag ttaaaccggt ttcgtctcgc
      481 aggagcggtt cgtagtctgg agccaaccac cagcgaggca tcgtgttttc ggggacacgc
      541 cctgaactgc taacaagtgt aggaaagctc ggtaagcccg caatcggggc gggctcgagc
      601 cctatggaaa tctgcttcat gcggaagtca gcggccacaa ggacacgggc aaagtgggta
      661 ttctcgggga ctcccgtcac agtcaccacc tgattaccca aaagctgttc caaagcggca
      721 gaaaaacgag cgggatcgac cccaccctgc cgcgatcggg cgaatgaccg tgcctttcgg
      781 atcccctccg ccgtgggatc gatcgagcaa ctaatgacgc tcctttgagg gccaaacgcc
      841 gaccgcatcg ccacaatcag gtcatccaga agaatgacag gctttcctga cttaatgccc
      901 accggataac cggtcgcatg gagtttccac ggctcagcgg gccccacgag cacgatgtcg
      961 ttcttatcgg gataaaccaa aacgtattca attcgcgtga gaccaccaag gcacgccact
     1021 tcatcgggta ccggctgacc agcctgaatc gccgcggcga gctggctttc tagaccacga
     1081 agtgagatgg cgcggctttc aacgggctga gcgagctttc ctgtcaattc cttgtgatat
     1141 gactgccaaa gctgacggat ttcggctaag ctaccgctgg gtgctttttg gatgaacccg
     1201 gatgggtcga tcataacccc accaacggcg cggtttgcat atacccctgt cgtctgggcg
     1261 attccgtggc ttgcacccac aattagcacc gcgaaggccg ccactgccgc gagagatttc
     1321 atccagccga accgacccca tgaactgacc ataagtgtct cccaatcgga aagccgtgtt
     1381 tctccgcgaa gataggtcgg agccaaacaa ccgaatcctg ccgaaaaccc tgccagggtt
     1441 tctcggcgtt tgaatatcac aatcccattt taggctaatt cagagacacg cacttttcaa
     1501 cctaaatcct cagtttttcc caaaccgtcc catttctgta gttcgggtgt ccaggtgccg
     1561 gattccaagt tgcggaacct actg
//
LOCUS       DAOPTR010000988         1582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_61152_length_1582_cov_3.436149, whole genome shotgun sequence.
ACCESSION   DAOPTR010000988 DAOPTR010000000
VERSION     DAOPTR010000988.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61152_length_1582_cov_3.436149"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PKI05_15165"
     CDS             <1..320
                     /locus_tag="PKI05_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HOA53593.1"
                     /translation="ADNITNKFWHVVLIAVPILVQVYFNSSLTYWLMYLFRVPHNIAS
                     PGALIGASNFFELAVATAIALFGPESGAALATVVGVLIEVPVMLSVCAFCNRTRHWFA
                     GFQ"
     gene            complement(673..>1582)
                     /locus_tag="PKI05_15170"
     CDS             complement(673..>1582)
                     /locus_tag="PKI05_15170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOA53594.1"
                     /translation="PRYNAKHGTKVGCFGLFESINDVRVAHALFSAAAKWLRQRGLTH
                     MMGPVDYSINYPCGLLIEGFQYPPRIMMNHNPPYYAELCESWGFQKAKDLYAWWFVKD
                     NENLRTWMKRVARLRQRSRITIRPFNLHDFENDMARCVEVYTATRYDQWGFIDLTPAE
                     FRYFAKRLAQFARPELVLVAEDNGKAVGFSITVPDLNEAIRPLNGRLTSWGMPIGAVR
                     LMQRLRHVKTARVMVLGVISGYRHRGIAELMIYETIKRGTEEANFEAAELSWTLEDNH
                     AINNMIASVGGKLYKRYRVYEKAIVD"
BASE COUNT          343 a          426 c          429 g          384 t
ORIGIN      
        1 aggctgataa cattaccaat aagttttggc acgttgtgct tattgctgtg cccatcttgg
       61 ttcaggtgta tttcaattcg tcgctgacat attggctcat gtatctcttc cgggtaccac
      121 acaatatcgc gtcacccggc gccttgatag gagccagcaa ctttttcgag ttggccgtgg
      181 caacggcgat cgcccttttt ggcccggaat caggagcggc cttggccacg gttgtcggtg
      241 tcttgatcga ggtccccgtg atgctttcgg tgtgcgcttt ttgtaatcgg acgcggcact
      301 ggtttgcagg ttttcaatag acctttcatc gaaacgcggg aggtgtctaa agcagcccgg
      361 cagccgctca acacgggaaa gtggtttaga gcctatccca caatctcctc tcgcccgttc
      421 ggcgggagaa gggtccggcg tgaaggtgcc tcgtatcact gccggtcttc tcaatcgatt
      481 cggacgttga cttgccctca ccccaaccct ctcccggagg gagaggggca gtttttagga
      541 taggctcaaa aacaagaagg cagttaacgg acagagctct tgattgttcc ggcttcgaca
      601 ggtgtccgtc tcatcagtcg tccagaggtg gaccaagcca tcgatgagga cgctgacggt
      661 ggcttatatc tttcaatcga cgatggcttt ttcgtacacg cgataccgtt tgtagagctt
      721 tccacccaca gaagcaatca tgttattaat ggcgtgatta tcttcgaggg tccaacttag
      781 ttccgcagct tcgaagttgg cttcttccgt gccgcgtttg atcgtttcgt agatcattag
      841 ttcagcaatg ccgcgatgcc gatagccgga gatgacgccc aggaccatga cgcgggcagt
      901 tttaacgtgg cgcaagcgct gcataagcct aacggcaccg atcggcattc cccaagaggt
      961 cagacgacca ttgagcgggc gaatggcttc attgaggtct ggcacggtga tcgaaaagcc
     1021 cacggccttg ccgttgtcct ctgccacaag caccaattcc ggtcgagcga actgcgccaa
     1081 tcgtttggca aaatagcgga attcggcggg cgtaaggtca ataaagcccc attgatcata
     1141 gcgcgttgcc gtgtaaactt ccacgcaacg cgccatatcg ttttcaaagt cgtgcaggtt
     1201 gaaggggcgt atggtgatgc gtgaccgttg ccgaagacgg gccacgcgct tcatccacgt
     1261 gcgaaggttt tcgttgtcct tcacgaacca ccaggcatag aggtccttgg ccttctgaaa
     1321 cccccaagac tcgcatagct cggcgtaata cggcggatta tgattcatca tgatccgcgg
     1381 cggatattga aatccttcaa tgagaagtcc acagggataa ttgatcgagt agtccacggg
     1441 ccccatcata tgggtcaaac cccgttgccg gagccatttg gcggctgccg aaaagagcgc
     1501 atgcgccact ctgacgtcgt tgatcgattc aaacagccca aaacagccca ccttcgtccc
     1561 atgtttcgca ttgtaacggg ga
//
LOCUS       DAOPTR010000989         1581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_61211_length_1581_cov_3.584535, whole genome shotgun sequence.
ACCESSION   DAOPTR010000989 DAOPTR010000000
VERSION     DAOPTR010000989.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61211_length_1581_cov_3.584535"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..1488)
                     /locus_tag="PKI05_15175"
     CDS             complement(46..1488)
                     /locus_tag="PKI05_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HOA53595.1"
                     /translation="MGKTWEYKDLKALLEPFQQTHLLAGWEDLSEEGRARLAAQIREL
                     DLPLIARLFRDRNLPIDIASLVERAVSPRGVRLDSEGAGLSPADAYRLGQGVLQRGEV
                     AALLVAGGQGTRLGFPHPKGMFPIGPISGHSLFQIHVEKVLARSRKAGCPIPLAVMTS
                     PATHQETVEFFSRHGRFGLPEEDLYFFCQGTMPSVDAATGKILLSAPGDLALSPDGHG
                     GMLAAIIRSGTLEKLASRGIKYLFYFQVDNPLVDILSPSAIGYHIYFGSEMSTEVVAK
                     ETPFDRVGNVIEVDGRLYVVEYSDLPDELAQKRSPDGRLLLWAGSIGVHIINVDFLFR
                     MYEQVDALPYHLAYKKVPYYDPASGPVTPTKPNAIKFERFIFDLIPHAKNAIVIEVNA
                     AQHFAPLKNAPGADRDTPEHVRQQMIALHRSWLERNQVAVCDGVAVEISPLFAVEPDD
                     LKGRFPPGYRITEPTFFSHTTWVSSAESRR"
BASE COUNT          375 a          446 c          447 g          313 t
ORIGIN      
        1 ctcatggggc cgtccggtgt ccccaccgca acaatcgacc gtagtttatc ttcttgactc
       61 ggcggaagaa acccacgtcg tatgcgaaaa gaaagtcggc tccgtgattc tgtagcccgg
      121 cggaaagcga cctttgaggt catccggctc aacggcgaac agtgggctga tttcgacggc
      181 gacaccatcg cagacggcga cttgattgcg ttccagccaa gagcggtgca gagcgatcat
      241 ctgttgccgg acatgctcgg gcgtatcgcg atccgcaccc ggggcgtttt tcagcggtgc
      301 gaagtgctgg gccgcattga cttcaatgac gatggcattc ttggcatgcg gaatgagatc
      361 gaaaatgaac cgttcaaact tgatcgcatt gggcttcgtc ggtgtcaccg gcccggacgc
      421 cggatcgtag taagggacct ttttgtaggc caaatgatag ggcaaggcgt caacctgctc
      481 gtacatgcgg aagagaaaat cgacgttaat gatgtgaacc ccgatacttc ctgcccacaa
      541 aagcaaccgg ccatcaggtg accgtttttg ggcaagctcg tcgggcaagt cgctgtattc
      601 gaccacgtaa agccgtccgt ccacctcgat cacatttccc acccgatcga agggcgtctc
      661 tttagcgaca acctcggtgg acatttctga accgaagtaa atgtgatagc cgatcgccga
      721 gggcgaaaga atatccacca ggggattatc gacctgaaag taaaaaaggt acttgatgcc
      781 gcgggacgcc agcttctcca acgtcccact gcggataatg gcagctaaca ttccaccatg
      841 gccatcggga gagagggcta aatccccggg ggcggaaagc aagatcttgc cggtggcagc
      901 atccacagag ggcattgtgc cctgacagaa gaagtaaaga tcctcttcgg gcagtccaaa
      961 tcggccatgg cgcgagaaga actcgacggt ctcctgatgg gtggccgggc tagtcatcac
     1021 agcaagtggg atggggcaac cggctttccg ggatcgcgca aggacctttt ccacatggat
     1081 ttgaaagaga gaatgtccgg agattggccc aatggggaac attcctttcg ggtggggaaa
     1141 gcccaagcgt gttccctgac cgccggccac cagcagtgcc gccacctcac cgcgttggag
     1201 cacaccttgg cctaagcgat aggcgtcggc cggggaaagt cccgcacctt cagaatccaa
     1261 acgaacaccc cgtggggata ccgcgcgttc gacgaggctg gcgatatcaa taggcagatt
     1321 gcggtcccga aagagtcgcg caatgagggg taaatccaac tcgcggatct gcgcagccag
     1381 ccttgcccgc ccttcctccg acaggtcttc ccaacctgcc aaaagatgcg tctgctggaa
     1441 cggctccagc aaggccttga gatccttata ttcccacgtt tttcccatcc tggaaacctg
     1501 ttgttgcgtt cggaccaaag actgaccgat cccgcagcca gtatttaccc gagatagtat
     1561 caccacgaaa taggttcaaa g
//
LOCUS       DAOPTR010000990         1581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_61214_length_1581_cov_3.420052, whole genome shotgun sequence.
ACCESSION   DAOPTR010000990 DAOPTR010000000
VERSION     DAOPTR010000990.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61214_length_1581_cov_3.420052"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PKI05_15180"
     CDS             <1..327
                     /locus_tag="PKI05_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOA53596.1"
                     /translation="EIHDAARIDWQLRGRCSRQGDPGTFRQFLALDDEILVKAFGAKK
                     AEKYAAIGRSRPGLFNEYLPIFRKAQRIMEKRHFRERRALMHFERERKKMQRQMGQDP
                     YLDTPG"
     gene            complement(449..>1581)
                     /locus_tag="PKI05_15185"
     CDS             complement(449..>1581)
                     /locus_tag="PKI05_15185"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HOA53597.1"
                     /translation="PGVRCLKLGDEEAPSVIYVQGYPLVFTGQVFPEAEEKGTIPDAL
                     EDQLALAWRRLEAILEKAGSQKEKIVRIGICVGQEDDAAPANAFVRQQFSKDWLPAIT
                     TVVTPLPYPKSRVAFDAVAYLEGQPEDVRLVRLSDDPNAPHDAAVCPQGGLVFFSGQP
                     DKNPLPRAVENAMKALFETAQNLHVEKKDILQIRVFLQPMSEAPAVLNELRKAFPEGA
                     MPPVIFVEWIASAPVEIEMIARLPAQADLGGDVVRFYNPPGVKPSATFSRVTLVRTDR
                     FIFFPALLSRKDGSGAEQVDDIFAQLIEGLKVVGSDIRHLLKATYYVVDKEASDAVDD
                     VRKKLYDPQRPPAASKVTIHGVGQAGRTVSVDMIAAPAVAAQ"
BASE COUNT          369 a          479 c          406 g          327 t
ORIGIN      
        1 gagatccacg acgcggcgcg gatcgactgg cagcttcgcg gtcgctgtag ccgccaaggt
       61 gatcccggca cctttcgcca attcctcgcc ctcgacgacg aaatcctcgt caaggccttt
      121 ggggccaaaa aggccgaaaa atacgccgcc attggtagga gccgcccagg gctattcaac
      181 gagtacttgc ccatcttccg caaggcacag cgaatcatgg aaaaacgcca cttccgcgag
      241 cggcgggcac tcatgcattt cgaacgcgaa cgaaagaaga tgcagcgcca aatggggcag
      301 gatccctact tggacacgcc gggttgagca aatcccaagc ctcagcctat gccctgcgtc
      361 gttggcagcc gagcaaaccc acagacgcat gtttcggctt tgcggagttt accgcgtgtc
      421 tccaagcgtc cttttccaga agaactcttc attgggcagc cactgccggt gcagcaatca
      481 tatcgacact gaccgtccgc ccggcctgac caacaccgtg gattgttacc ttcgacgccg
      541 cagggggccg ctggggatca taaagctttt ttcgcacgtc atcgacggca tcactggcct
      601 ctttatcgac cacataatat gtggccttta gaaggtgacg gatgtcactc cccaccactt
      661 ttaaaccctc tatcaattgg gcgaagatat catccacttg ttctgcacca gaaccatctt
      721 ttcgcgacaa caacgccgga aagaagataa accggtcggt gcggaccaag gtaacccggc
      781 tgaaggttgc cgacggtttg acacccggcg gattgtagaa acgaacgaca tcaccaccca
      841 gatcggcttg ggccggtaat cgagcaatca tttcgatttc cacaggtgca gaggctatcc
      901 attccacgaa gatcaccggg ggcatggcac cttcgggaaa ggcctttcgc aactcgttga
      961 gcacagcagg ggcctcactc atcggttgaa ggaagacacg gatttggagg atgtctttct
     1021 tctccacgtg gaggttctgg gccgtctcaa ataaggcctt catggcgttt tccaccgccc
     1081 gcggcaaggg atttttgtca ggttgcccag agaagaacac caaccctccc tgcggacaca
     1141 cagcagcatc gtgtggtgcg tttggatcat ctgacaaccg gaccaacctc acatcctccg
     1201 gttggccttc caaatacgct acggcatcaa aggccacacg cgatttgggg tagggtaggg
     1261 gagtcaccac cgtagtgatg gccggcagcc aatccttcga aaattgctgt cgaacaaacg
     1321 cgtttgccgg cgcagcatcg tcctcttgcc caacgcagat accaatacgg acaatcttct
     1381 ccttttggga gcccgctttc tcgagaatgg cctcaagtcg cctccatgct aaggccagtt
     1441 gatcctccaa ggcatcaggg atcgtaccct tctcctcggc ctcgggaaag acctgtcctg
     1501 taaagacaag aggatatcct tgcacataga ttacagacgg ggcctcctca tcacccaact
     1561 tcaagcatcg tacccctggt g
//
LOCUS       DAOPTR010000991         1579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_61341_length_1579_cov_3.620079, whole genome shotgun sequence.
ACCESSION   DAOPTR010000991 DAOPTR010000000
VERSION     DAOPTR010000991.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61341_length_1579_cov_3.620079"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PKI05_15190"
     CDS             complement(<1..351)
                     /locus_tag="PKI05_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA53598.1"
                     /translation="MRLGFLVPLGLAVVGFVVQAASVATSAEQGQRPNILFIMADDHA
                     YQALSCYGYGLNQTPNLDRLAKEGMRFQNCFVTNSICGPSRAVILTGKYSHLNGFARN
                     GDVFDGTQQTVSKLL"
     gene            complement(528..>1579)
                     /locus_tag="PKI05_15195"
     CDS             complement(528..>1579)
                     /locus_tag="PKI05_15195"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOA53599.1"
                     /translation="GWNVVWGPEEDLETAAKHGLRLQLQDPLLSPASLDDPERRAKLD
                     ALIERVRSHPALYSYFLRDEPSAGDFPALGRLVEYLRQRDPMHLAYINLFPTYATNEQ
                     LGTKGDVVTAYREHLRLYLEIVKPDLISYDHYQFRANSDGDQYFLNLSLIREFALASK
                     LPFLNIVQACSWTPSMRVPNGDELRYLVYTTLAYGAQGISYYVYCWPNHVGGMANPDG
                     TPTPLYHAAKVLNREFAAIAEELQPLTSLAVYHVGNLPPGAVALPAEAPFRITSKSDA
                     AAGFLLGYFGQADRSPTHVVVVNLNYRSPVVGELTGPAPLERFDPHERRWEPLSASTV
                     SLDFLPGGGFLLRTR"
BASE COUNT          383 a          423 c          450 g          323 t
ORIGIN      
        1 cagcagcttg gataccgtct gctgggtgcc atcgaagacg tcgccgtttc gtgcgaaccc
       61 attgaggtgg gaatattttc ctgtcagaat gacggcccga cttggaccac aaatcgaatt
      121 ggtcacaaaa caattctgga agcgcatccc ttcttttgcc aagcgatcga ggttgggcgt
      181 ttgattaagt ccatagccat aacagctcaa cgcctgatag gcatgatcgt ccgccatgat
      241 gaaaaggatg ttaggacgct gcccttgttc ggctgacgtc gctaccgagg ccgcttgcac
      301 aacgaagcca acaacggcca acccaagagg tacaagaaaa ccaaggcgca taattcacct
      361 ccccaaaagc gatcaaagtc ttgtcgatga ctcactcaat ccgttccgaa atcaccctaa
      421 cgttgttacc ggatagtgtg gcttgagcac cggtcattgt caaccgacga agggataact
      481 ttaccagcgg ttcataacgc tattagatag gaaacccaac aaaacgacta tcgggttcga
      541 aggagaaaac ctccgcccgg cagaaaatcc agcgataccg tggaggccga caaaggctcc
      601 caccgtctct cgtggggatc gaatcgctca aggggggctg gtcctgtgag ttcccccacg
      661 accggcgagc gatagttcag attgacgacc acaacgtgcg tcgggctccg gtctgcctgg
      721 ccaaaatacc ccaagaggaa accggccgcg gcatcgctct tagaggtaat ccggaacggc
      781 gcttccgctg ggagagcaac tgcccctggc gggagattcc caacgtgata gacggccagc
      841 gatgtcaacg gctgcaattc ttctgcaatg gcggcaaatt cgcgattgag aaccttggcc
      901 gcgtggtaca gtggggtcgg cgtgccgtcg ggattggcca tgccgcccac gtggttgggc
      961 cagcagtaca catagtaaga aatcccttgc gcaccatacg ccaaggttgt gtaaacgagg
     1021 tatcgcagtt catcaccgtt aggtacccgc atggagggtg tccaactgca cgcctgaaca
     1081 atgttaagga aggggagttt cgaggccaac gcaaattcgc ggattagcga aagattaaga
     1141 aagtactgat cgccgtcact gttcgcgcga aattggtaat gatcgtagct gatgaggtcc
     1201 ggtttgacga tctcaaggta aagacggaga tgctcgcggt aggcggtgac gacgtctccc
     1261 tttgtgccaa gttgttcgtt tgtggcatac gtcgggaaaa ggttaatgta ggcgagatgc
     1321 attggatcgc gttggcgaag gtattccacc aggcgcccta aggcgggaaa atcgcctgcg
     1381 cttggttcgt cccgcaagaa atagctgtaa agggcgggat gactgcgaac gcgctcgatc
     1441 aacgcgtcga gtttcgcccg ccgttccgga tcgtcgagtg acgccggcga gagaagcggg
     1501 tcctgaagtt gcaagcgtag tccgtgcttg gccgccgtct ccaagtcttc ttccgggccc
     1561 cagacaacat tccaaccac
//
LOCUS       DAOPTR010000992         1578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_61399_length_1578_cov_4.188444, whole genome shotgun sequence.
ACCESSION   DAOPTR010000992 DAOPTR010000000
VERSION     DAOPTR010000992.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61399_length_1578_cov_4.188444"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PKI05_15200"
     CDS             <1..734
                     /locus_tag="PKI05_15200"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOA53600.1"
                     /translation="QIDLLGYVVEVGPIKFPAKEGHLDMLRQLRDSSELYTLHPEGEE
                     EAKDYIRKCQKEGDTVGGVVEVRVENVPFGLGTHAQWDRKLDGRLAQAVMAIQAIKGV
                     EIGLGFEAARRPGSQVHDPIHFDPSQVNSRTLGFVRATNHAGGLEAGMSNAQPIVLRA
                     AMKPISTLARPLESVNLETKQPETAAYERSDVCAISAASVIVENVVAFEIAAALVDKF
                     GGDHIEEIRQRIKLFEQMNRERLGL"
     gene            867..>1578
                     /locus_tag="PKI05_15205"
     CDS             867..>1578
                     /locus_tag="PKI05_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53601.1"
                     /translation="MRDSVAKVMLIGILLLIWTVPLALILGFTLWWRPGMGIRTECDR
                     IAHAIGFSCAIERYQTPRPGVKRYSHLTLRNTLTGSPVCVIENALSTRKSNKPHIDAS
                     GVSLSEEAWLRLWEICEEWLLGKRDFAAETFTLTASEIQVVPKLDEARPNPQPLVLCS
                     SLQTEFSSPSRQRAIKCRIVCSAEDQRQPPLPDLRPSDGPLQSTLSMVWQSTCKDGQF
                     YLTKQVRVEEPGIPLVVLK"
BASE COUNT          383 a          408 c          444 g          343 t
ORIGIN      
        1 gtcagattga ccttttggga tacgttgtcg aggttggacc gatcaaattc ccggccaagg
       61 aaggccatct cgatatgtta cgacaacttc gggattcgtc cgaactttac acgctacacc
      121 cggaaggtga agaggaggcc aaggactaca tcaggaaatg ccaaaaagag ggtgacacgg
      181 tgggcggcgt ggtcgaagtt cgtgtcgaaa atgtaccctt tggattgggg acccatgccc
      241 agtgggaccg aaaattggac gggcggttgg cacaggctgt aatggctatt caggctatta
      301 agggcgtcga aattggcctg ggattcgagg ccgcccgacg gccagggtca caagttcatg
      361 accctatcca ttttgatcct tcgcaggtaa acagccggac gcttggtttt gtgcgcgcca
      421 ctaatcatgc ggggggcctc gaggcaggca tgagtaacgc ccagcctatt gtgttgaggg
      481 cggccatgaa accgattagc acactggcac gcccgctgga gtccgttaat ttagagacca
      541 agcaacccga gacggccgcc tatgaacgga gcgacgtatg tgccatctca gcagccagcg
      601 taatcgtgga aaatgtcgtg gcttttgaaa ttgcggcggc tctcgtcgac aaattcggcg
      661 gggaccacat cgaggaaatc cgccagcgta taaagctttt tgagcaaatg aaccgggaaa
      721 ggctaggatt gtaaccgcgc agcatttcta gaagcagctg ccgggaattg ggaccgtgtg
      781 gcagcggcta cgcttgctgt tcgggcacct ttccaaaggg cctttctaca caggagaatc
      841 gcaccggcgt cacggaggtc atgcgcatgc gggattcggt agcaaaagtg atgctgattg
      901 gaatcctgct cctgatatgg actgtccccc tcgcgctgat cctcggtttt accctctggt
      961 ggcggccagg catgggcatt cgtaccgagt gcgacagaat agcacatgct ataggcttct
     1021 cgtgtgctat cgaacgctat caaacacctc gacccggcgt gaaacgatac tcacacctga
     1081 ctctccgcaa taccctcacg gggagcccgg tttgcgtgat tgaaaacgcg ttgtccacaa
     1141 gaaaaagcaa caaaccgcat attgacgcct ccggcgtttc tttgagtgaa gaagcatggt
     1201 tacgcctttg ggaaatctgt gaagaatggt tattgggcaa acgtgatttt gccgcagaga
     1261 cattcacact cacagcgagt gaaatccaag ttgtccccaa actcgatgaa gccagaccaa
     1321 atcctcaacc tcttgtgtta tgttcgtcac tccaaaccga gttttcatcg ccgtcccgcc
     1381 agcgtgctat caaatgtcga attgtctgtt cggcagaaga ccagcggcag ccgccgttgc
     1441 ccgacttgcg cccgtccgac ggtccattgc aaagcacgct aagtatggta tggcaatcaa
     1501 catgcaagga tgggcagttc tacctgacaa aacaggtaag agtggaggaa ccgggcattc
     1561 ctctggtggt tctaaaac
//
LOCUS       DAOPTR010000993         1576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_61500_length_1576_cov_3.976331, whole genome shotgun sequence.
ACCESSION   DAOPTR010000993 DAOPTR010000000
VERSION     DAOPTR010000993.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61500_length_1576_cov_3.976331"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="PKI05_15210"
     CDS             complement(<1..1297)
                     /locus_tag="PKI05_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53602.1"
                     /translation="MLTAILAFVLLASPLAGQERTGTPPSDVFGQRRPLLQIDEAKLL
                     AGGLQKITAKYITLYTDCPLTQEILQLGEVFDAAYPQWCEYFGLTPGSRPWRMQGLLM
                     KDAQRFRQLGVLPEWLPPFTNGYSVGDILWCYDQASDYYRRHLLLHEGTHGFIRAHFG
                     DGVPPWYNEGIAELLGTHFWNGSRLILGYVPRTREEVPLWGRVKIVRDAVRAGQPLSL
                     AEVLAFGPTAHREVGPYGWCWAATALFDRDARFSPVFRAAVGWVNRPDFQQQFLNHLG
                     NHWRDAQWQWMILLSNLDYGYDFDREASDFARGQPLPPHQVMVRVVADRGWQNSGWYL
                     EAGRTYSLTARGRFVVVQDDPPWESEPQGVTIRYVRGKPLGLLMAAIVPDEPTEPQAE
                     LTTVGSFFQPYPVGRHLLFSPTRSGTLFLRINDAPAELAD"
BASE COUNT          365 a          490 c          438 g          283 t
ORIGIN      
        1 tatcggccaa ttcggcaggt gcgtcgttga ttctgagaaa aagcgtcccg ctccgcgtgg
       61 gtgaaaataa caagtggcgc cccacaggat aaggttgaaa aaagctcccg accgtggtca
      121 actccgcctg aggctcggtg ggctcatcag gcacaatggc cgccatgaga agacccaggg
      181 gtttccctcg cacatagcgt atcgtcaccc cttggggttc gctctcccag ggagggtcgt
      241 cttggacgac gacgaatcgg ccacgggcag tgagcgagta cgtgcgcccc gcctccaaat
      301 accagcccga gttctgccaa cctcggtcgg caaccacacg gaccatgacc tgatgaggtg
      361 ggagaggctg cccccgggcg aaatcgctgg cttcccgatc aaagtcgtag ccgtaatcca
      421 gattgcttag caggatcatc cattgccatt gggcatctcg ccagtgattt cccaagtgat
      481 tcaaaaactg ctgctggaaa tcggggcgat tgacccagcc tacggcagcc cgaaagaccg
      541 gagaaaaccg cgcatcccga tcaaaaaggg ccgttgccgc ccaacaccaa ccgtatggtc
      601 ccacctcgcg atgggccgtc ggcccgaagg ccagcacctc ggccagcgac aacggctgac
      661 ccgcccgcac agcgtctcga acgatcttca cgcgtcccca aaggggaact tcctctcgcg
      721 tccgcggcac ataaccaagg atcaatcgac tgccgttcca aaagtgagtc cccaataact
      781 cggcaatccc ctcgttgtac cacgggggca caccatcccc aaaatgggcg cggataaagc
      841 cgtgggtccc ctcgtgaaga agcaaatggc gccggtaata atcgctcgcc tggtcgtaac
      901 accaaaggat gtcgcccacc gaatacccgt tggtaaaagg cggcaaccac tctggaagga
      961 cacccaattg gcgaaaccgc tgggcgtctt tcatcaacaa gccctgcatt cgccaggggc
     1021 ggctccccgg cgtgagacca aagtattcgc accactgcgg ataagcggca tcgaagacct
     1081 cgcccagttg gaggatttcc tgcgtgaggg ggcaatccgt gtagagcgta atgtactttg
     1141 ccgtgatctt ctgcagaccg ccggcgagaa gcttggcctc atcaatctgc agcagcgggc
     1201 gccgctggcc aaagacgtcg cttgggggtg ttcccgtccg ctcttgtcct gccaaaggac
     1261 tggccagcaa cacaaaagcc aaaatcgcgg taagcatgaa aggccccaac gtcctcaggg
     1321 gcatacggcc cggtgaaagc gggctgttct cgctcgaagc tatagcgcat ggacgtgtcc
     1381 ctttgagtgg caggacaaat ccgcagcgcg ggaagtgccg tctcttgctg cctctcggaa
     1441 ctcctatcag cctgtcagac atcaggagga tcccagtatg acaatctttt gatcgagttg
     1501 agacaactga cggtcatcgc ctgaaacacg gcagccagtt tctcgtaatc ccgatcccag
     1561 ccttggctga agaaca
//
LOCUS       DAOPTR010000994         1576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_61512_length_1576_cov_3.085470, whole genome shotgun sequence.
ACCESSION   DAOPTR010000994 DAOPTR010000000
VERSION     DAOPTR010000994.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61512_length_1576_cov_3.085470"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PKI05_15215"
     CDS             <1..299
                     /locus_tag="PKI05_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53603.1"
                     /translation="VPAKKPGVWKKLLSMGKAGSDSSIIEEAVQDAISRTGKGYRGKG
                     LKQLIDAVRAYDDGNLIILSNRGSYMFTNAKVQLRDYGNSIFGTLIQWRVPITR"
     gene            310..639
                     /locus_tag="PKI05_15220"
     CDS             310..639
                     /locus_tag="PKI05_15220"
                     /inference="COORDINATES: protein motif:HMM:NF025574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS-like domain-containing protein"
                     /protein_id="HOA53604.1"
                     /translation="MSTRVIKVAGDFSRYPAGRYKNDGPYSGEAFRDNFLLPAVSAGE
                     QVVIDLDGVRGYGSSFLEEAFGGLVRKGFSQATIKSLLLLRSIDKSLIEEVEDYIQHG
                     AEPDQDV"
     gene            complement(1292..>1576)
                     /locus_tag="PKI05_15225"
     CDS             complement(1292..>1576)
                     /locus_tag="PKI05_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HOA53605.1"
                     /translation="RDGAVAWGDDHPRDAILQEIEAKGLASWKNDSGYHRRSIAENMM
                     FRFKRLDDTLFSRRFERQVNEAHVRAAIINTFTCLGMPQSARVGQVAPAA"
BASE COUNT          366 a          353 c          448 g          409 t
ORIGIN      
        1 ctgttccggc aaagaaacct ggtgtgtgga agaaattgct ttctatgggc aaggcaggta
       61 gcgatagtag tattatcgaa gaggctgtgc aagacgccat atcgcgtaca gggaaggggt
      121 atcgaggaaa aggtctcaag caactcatcg atgctgttcg tgcttacgac gatggcaatt
      181 taataatcct gagcaatagg gggagttata tgttcactaa tgcaaaagtt caactgcggg
      241 attatggaaa tagtattttt ggaaccttga ttcagtggcg ggttccaatt actcgatgag
      301 ggccctttga tgtcaacacg tgtaataaaa gtagctgggg acttctctcg ttatccggct
      361 ggacgttaca aaaatgacgg gccatatagc ggggaagcgt tccgagataa tttcttgcta
      421 ccggccgtat ccgctggaga acaggtggtt attgatcttg acggcgttcg gggctacgga
      481 tcatcgttcc ttgaagaggc ttttggtggc ctcgttcgca aggggttttc gcaggccact
      541 attaagagtc tcctattgct gcgctcaatc gataagagcc tgatcgagga ggtcgaggat
      601 tatatccaac acggggccga gccagaccag gatgtgtgat gtcaggtaca caatactgaa
      661 tcaaataggg acttggcttg tcgtcgtcac cggatggctt gtcgtccact tttttacagt
      721 gatgcgagag aggcaaaaag aaatccgaga attaaaatca agacttgttg aacgtgtatt
      781 agaaatcgag aaacgatcaa tatgctttca tcaggcgcaa agctatagtt acgagaacgg
      841 cgcgtggcta gtcgctgaaa ttgatcgagt tagcaagact ctttcccgtc gcccattgtc
      901 atccttagca gtcaggccgg aagtaatcaa aagatttcga cagagtataa ctcggagaaa
      961 ctttgatttc actagtttcg tgccacagct atcaaatagc gagttgattt tcgccatctt
     1021 tgcgggcgcg ctggatctta cagaagaatt ggagaaggcg tacgagtcca gatatctcga
     1081 ttcttggtgg cagctcttta gggattaggg tcggcggatc cgaactggtg agggttttct
     1141 gtcatgtttt tgtccccgat gtcaacgaat tcaggctggt tagtggcgcg gcgtgttgct
     1201 ttcggcgttg gtgagctatt agcaatgact aatgctgaac gaaactaggc tcgtcatttt
     1261 cggcatggtc ccccccattt cccgtcctcc gtcatgccgc aggcgccact tgccctaccc
     1321 gggcggattg cggcatgccc aggcaggtga aggtgttgat aatagccgcc cgaacgtggg
     1381 cttcgttcac ttgtcgctcg aagcgccggg agaacagtgt gtcgtcgagt cgcttgaacc
     1441 ggaacatcat gttctcggcg atgctgcgcc ggtggtaacc gctgtcgttc ttccagctgg
     1501 cgagtccctt ggcttcgatt tcctggagga tggcgtcgcg ggggtggtcg tctccccacg
     1561 caaccgctcc atcgcg
//
LOCUS       DAOPTR010000995         1570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_61842_length_1570_cov_3.915512, whole genome shotgun sequence.
ACCESSION   DAOPTR010000995 DAOPTR010000000
VERSION     DAOPTR010000995.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61842_length_1570_cov_3.915512"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="PKI05_15230"
     CDS             <1..930
                     /locus_tag="PKI05_15230"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOA53606.1"
                     /translation="GYEAVFLGVGAPEGTRLGIPGEDVQGVEDALTFLRTYNIRGSVR
                     VGKRVVVVGGGNSAIDAARTAIRLGAEMVTVVYRRNREAMPAYEEEITEAEAEGVRLQ
                     LLTAPVEVLSEGGQVVGLKCVPVQLGEFDRSGRRRPKEVGEAFCIPADQILVAVGQTL
                     DLERLVPDVELRRNSRGFIEVDPVTGQTSHRWIFAGGDAVNGPSSVVEAVAAGERAAV
                     GIDMLLTGQSHAFWRETKPVETSFDPDAEPEATPREPLRLLPVERRRHNFDEVELPWP
                     EPVALRQARRCLRCDYGKRNGVQGPVLHQVGEL"
     gene            1010..>1570
                     /locus_tag="PKI05_15235"
     CDS             1010..>1570
                     /locus_tag="PKI05_15235"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOA53607.1"
                     /translation="MVRIYINDQEYDVPAGTTILCAAKKAGIRIPTLCFVEGLRPIGA
                     CRVCVVEVEGVRNLVASCSTPVAEDMRIHTHTARVRRARRAVVELLLSEHNGECQTCE
                     RNGQCELQSLAAELGIREVSYRGEKARKRIDISTPALQRDSGKCIKCRRCVSVCNEMQ
                     HVGALFPQFRGYQTIIGPALGRDLASV"
BASE COUNT          333 a          397 c          496 g          344 t
ORIGIN      
        1 ggatacgaag cggtctttct tggagtaggg gcgcccgaag gcacgcggtt aggtattccc
       61 ggtgaagatg tccaaggtgt cgaagacgca ttgacgttcc tccgcacata caacattcga
      121 gggtcggtac gcgttggaaa gcgagtggtc gttgtgggtg gcggcaactc cgcaattgat
      181 gctgcccgca cggccattcg gcttggggct gagatggtga ccgtggttta ccgccgtaat
      241 cgtgaagcga tgcccgccta tgaagaagaa atcaccgagg cagaagcgga aggcgtgcgt
      301 cttcagctcc ttaccgctcc tgtggaggtc cttagcgagg gagggcaggt cgttggacta
      361 aaatgcgttc ccgttcaatt aggagagttt gaccgatccg gaagacgtcg gcccaaagag
      421 gtcggcgagg cgttttgtat ccccgctgat cagatcttgg tcgcagttgg tcaaacgctc
      481 gatttggagc ggcttgtccc cgacgtggaa ctgcgccgta attcgcgtgg gtttattgag
      541 gtcgatcccg tcacgggtca aacatcccat cgctggattt ttgccggagg tgatgccgtc
      601 aacggtccgt cctcggtggt tgaagccgtg gcggctgggg agcgagccgc cgttggcatt
      661 gacatgttgc taacagggca gtcgcacgcc ttttggcgag agacaaaacc tgtggaaact
      721 agctttgacc ctgatgcaga gcctgaggcg actccccgcg agccgctgcg cctccttccc
      781 gtcgagcgac ggcgccacaa ctttgacgag gttgagctac cgtggccaga gccggttgcc
      841 ctccgtcagg cacgacgctg cttaaggtgt gattatggaa agcggaatgg cgtgcaagga
      901 cccgttctcc atcaggttgg cgagctgtga atcaggtacc gtgccttgca ataagcacgc
      961 aaagaagaca ctcgccaagc caaggatcca cgatgagtaa ggtgttgata tggtaaggat
     1021 ttacattaac gatcaagaat atgacgttcc tgccggaacg accatcttgt gcgctgcaaa
     1081 gaaggccggc attcgtattc ccaccttgtg cttcgtagaa ggtctgcgac ccatcggggc
     1141 ctgccgtgtg tgcgttgttg aggtggaggg ggttcgcaac ctagtcgctt cgtgttccac
     1201 acctgttgcc gaagatatgc gtattcatac gcacacggcg agggttcgtc gagcacgaag
     1261 ggccgtcgtg gagttgttac tttcggagca caacggcgag tgccagacct gtgaacgaaa
     1321 cggacaatgt gaactccagt ctctcgcggc ggaattgggg atcagagaag ttagctatcg
     1381 aggcgagaag gcccgcaaga gaattgacat ctcaacgccg gctttgcaac gggatagcgg
     1441 caagtgtatc aagtgccgcc gatgcgtaag cgtatgcaat gagatgcagc acgtgggtgc
     1501 cctatttccc cagtttcgcg gttatcagac aattattgga ccagcgctgg gacgtgacct
     1561 tgctagcgtt
//
LOCUS       DAOPTR010000996         1570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_61843_length_1570_cov_3.794719, whole genome shotgun sequence.
ACCESSION   DAOPTR010000996 DAOPTR010000000
VERSION     DAOPTR010000996.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61843_length_1570_cov_3.794719"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PKI05_15240"
     CDS             <1..189
                     /locus_tag="PKI05_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53608.1"
                     /translation="EHGGGRSFRDGFRAKWGTEQTRSNSAEANCRQLFGQWDCTRADG
                     RSHYPQIGTSRRQPCVVG"
     gene            179..>1570
                     /locus_tag="PKI05_15245"
     CDS             179..>1570
                     /locus_tag="PKI05_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53609.1"
                     /translation="MLVETSGPREITLGKPGLYEVFVRNIGRGVGNQVRVTVALPSWA
                     EVVEQVPTAGTVESTTNAQKQDLVWMIQQIDPQKEERLRLKLIPRESKPIELAVQYTV
                     LAPVNETKIEVREPKLAIRVDGPEQARFGRAEMYRLEVVNTGTGEADNVVLSLLPVGT
                     GQKTPARQTLGSIPAGEKRVVEVELIPRQTGAIQIQIEVTADGGIRTQLEHVVTVIRP
                     ALEVLVTGPAMQFVNQDDRYQVVVRNPGTAPAENVRVQVTCPESVEPDLTALKGAKPV
                     GDVLEWSIPSLPAGGEVVIDIPCRFVGMGDARLMVQATAEENLTTQGECLTQIQAVAD
                     LALRVEDPPRPVPVGKDGLYQLIIENRGSKAATDVRVVGFFSEGIEPIAAKGIPHRVL
                     PGQVVFEPISKIEPGERIVLEIQARAHKPGNHVFRAELRCPSSDIRLAAEETTRFYDA
                     TIAERPADSSSKLR"
BASE COUNT          344 a          429 c          513 g          284 t
ORIGIN      
        1 gagcacggcg gcggtcggtc gttcagagac ggattccgtg ccaagtgggg aaccgaacaa
       61 acccggagta actccgccga ggcaaactgt aggcagcttt tcgggcaatg ggactgcacg
      121 cgagcggacg gccgatctca ctatccccag atcggtacaa gccgtcggca gccctgtgtt
      181 gttggttgag acgagtggtc ctcgcgagat cacgctgggt aagccgggtt tgtatgaggt
      241 tttcgtccgg aacatcggtc gcggcgtggg aaatcaggtc cgcgtgaccg ttgccctacc
      301 ttcttgggcc gaggtcgtgg aacaggtccc gacggccgga actgtcgaga gcacgaccaa
      361 cgcccagaaa caggacctcg tgtggatgat ccagcagatt gatccccaaa aagaggaacg
      421 tctgcgattg aaactcattc cgcgggagag caagcccatt gaactggcgg tgcagtacac
      481 ggttttggcc cctgtgaacg agacgaagat cgaggttagg gagccaaaac tagcaatccg
      541 tgtggatggc cccgagcagg cccggttcgg tcgtgcggaa atgtatcgtt tggaggtcgt
      601 caacacgggg accggagaag cagacaacgt ggtcctctcc ctgctgccgg tgggaacagg
      661 ccaaaagacg ccggccaggc agacgctcgg ttcaatcccg gcgggtgaga aaagggtggt
      721 cgaggtcgag cttatccccc gtcagacagg agcgatccaa attcagatcg aagtgacagc
      781 agatggtggg attcgcactc aacttgaaca cgtggtgacg gtcatccgac cggcgctaga
      841 ggtgttggta accggtcccg ccatgcagtt cgtgaatcaa gatgaccggt atcaagttgt
      901 ggtgcggaat cctgggacgg caccggcgga aaacgtccgc gttcaggtga cctgtcccga
      961 atccgtcgaa cctgatttga cggccctaaa aggcgccaag ccagtcggcg acgtgttaga
     1021 gtggtcgatc ccgtctttgc cggcgggcgg cgaggtcgtg atcgacattc catgtcggtt
     1081 cgtcgggatg ggtgatgccc gattgatggt ccaggccacg gcggaagaga atttgaccac
     1141 ccagggagaa tgcctgacac aaatccaggc ggtggcagac ctagcacttc gcgtggaaga
     1201 cccaccgcgg ccagtcccag tggggaaaga tgggttgtat caactcatca tcgagaaccg
     1261 cggcagcaag gcggccacgg atgtccgagt cgttggattc ttctccgagg gaatcgagcc
     1321 aatcgcggcc aaggggatcc cgcacagggt cttgcccggt caggtcgtgt ttgagcccat
     1381 cagcaagatt gagccgggtg agcgaattgt gctggaaatt caagcgcggg cgcacaagcc
     1441 gggaaaccat gtcttccgcg cggaactgcg atgcccaagc agcgatatcc gtcttgccgc
     1501 ggaggaaacc acgcgctttt acgatgccac gatcgccgag cggcccgcgg attcatcctc
     1561 gaagctccgc
//
LOCUS       DAOPTR010000997         1566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_62086_length_1566_cov_3.288551, whole genome shotgun sequence.
ACCESSION   DAOPTR010000997 DAOPTR010000000
VERSION     DAOPTR010000997.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62086_length_1566_cov_3.288551"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="PKI05_15250"
     CDS             complement(<1..672)
                     /locus_tag="PKI05_15250"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOA53610.1"
                     /translation="MIDRENASLDLREAEAPEVVPREGLGRRVGKVFQILAMLCVLGL
                     FALVTIKYSPFNPDRVPVGVNHPSVGKQLEVISLTPLYGEGGILDRSGVSGKVVLLNF
                     WGTWCPPCRAEFPELVSLVRKFAHDSRFQFLSVSCAPPGADGDLEELRQSTQAFLLEQ
                     NASDVSIYADPDYLTRREVDRVAGFSVYPTTILLDEKGVIRAVWQGYWRGLDREIEES
                     LRHVLA"
     gene            complement(719..1210)
                     /locus_tag="PKI05_15255"
     CDS             complement(719..1210)
                     /locus_tag="PKI05_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53611.1"
                     /translation="MSIADTATPKTQYWQPLSKADPQPDNPVAAAIWQVISRIYPNAP
                     QTERRQAPRYAFPYLVQIQPVFEDGVSRYGSPFVVVGKQISELGLGFYHPKPLPSRYG
                     IVSLDAGGTQVSLLIELLWTQFTHHGWYESGGRFVRLLGSPHSNGNMTGVHAAQTADR
                     MNF"
BASE COUNT          418 a          444 c          388 g          316 t
ORIGIN      
        1 tgccaatacg tggcgcagcg actcttcaat ctccctatcc agtcctctcc agtaaccctg
       61 ccacacagcc cgaataacac ccttctcatc gaggaggatt gtggtgggat aaaccgaaaa
      121 gccggccacc cgatcgacct ctcgacgcgt aaggtagtca ggatcggcgt agatggagac
      181 atccgaggca ttctgttcca acagaaaagc ctgcgtagat tggcgtaatt cctctagatc
      241 accgtctgca ccgggtggag cacacgagac cgagagaaat tggaatcgcg aatcgtgagc
      301 gaatttccgc acaagcgaca ctaactccgg aaactctgcc cgacagggtg ggcaccacgt
      361 cccccaaaaa ttaaggagca caactttgcc cgatactccg gagcgatcga ggataccacc
      421 ttctccatag agcggcgtta gggatattac ttcgagttgt ttaccgacac ttggatggtt
      481 aacgccaaca gggacccggt ccgggttaaa cggggaatac ttgatcgtta ccagggcgaa
      541 aagccccagc acacaaagca tggcgaggat ttgaaacact ttaccaaccc gacgccccaa
      601 accctctcgt gggaccacct caggggcctc tgcttctcgc aaatctagac tagcattttc
      661 acgatcaatc atacgcggcg gcacaacacg caccaacgga catgcaaatg agagtcgttc
      721 agaaattcat tctatccgcg gtctgcgccg catgaacacc cgtcatgttt ccattagagt
      781 gaggggaacc cagcaaccgg acaaatcggc caccactttc ataccatccg tggtgagtga
      841 actgagtcca aagtaactcg atcaacaggg aaacctgtgt ccctccggcg tcgagtgaaa
      901 caattccata acgacttggc agcggctttg gatgataaaa ccccaacccc agctctgaaa
      961 tctgtttgcc aacgacaaca aagggtgaac cgtaacggga gaccccatcc tcgaaaacag
     1021 gctgaatttg aacaagatac ggaaacgcgt aacgtggtgc ctgccgccgc tccgtttgag
     1081 gcgcattcgg ataaatccgc gaaatgacct gccaaattgc tgctgccaca ggattgtcgg
     1141 gctgcgggtc agctttcgat aagggttgcc agtattgcgt cttgggcgta gccgtatccg
     1201 ctatgctcat gctcgtccct cccttacagc gatcctatgg gcggcggcag aaagacatcc
     1261 taaaacaggt ttcgccgatg atgccgcacc ctgaaactcg ttggttattg cgctgacgcg
     1321 acgggcacag ctcggagtca ttccaaacca gttttccggg cgcgccctcc aaaagaacca
     1381 gcttctggga taaggttccg tgatgccgct tcccaaaaac tcgccaagta aaacctaggt
     1441 catgaatccc cgactgtcaa atacggagct accaggcgtt agtatcacgg ccgatcagtg
     1501 aatggaatag cgtgaaatcc cgttgaaagc atcagaaaag gagtcattta taggcagttc
     1561 aataag
//
LOCUS       DAOPTR010000998         1565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_62136_length_1565_cov_3.391391, whole genome shotgun sequence.
ACCESSION   DAOPTR010000998 DAOPTR010000000
VERSION     DAOPTR010000998.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62136_length_1565_cov_3.391391"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PKI05_15260"
     CDS             <1..555
                     /locus_tag="PKI05_15260"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOA53612.1"
                     /translation="ELFCELSDPPLSSVALNAEAAGYRAAALLDRMMLGEKIAPQEIV
                     AEALHVVTRRSSEIVAMEDPDLTAALQFIHDHVTEPITVADVVSAVHVSRRALELRFR
                     RQLGRTIHEELERLRLQRAERLLIETELPIPYVAEAVGYNSPNYFARIFQQKRLMSPT
                     EFRRQKRGELLRQDLRVWKTMIAE"
     gene            complement(582..1469)
                     /locus_tag="PKI05_15265"
     CDS             complement(582..1469)
                     /locus_tag="PKI05_15265"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOA53613.1"
                     /translation="MVFVPFLFCLRMKHDQDLGESKWTWQIRELIEGMAMRIGAMNHP
                     AKDVIEEIRWIAENGFDFVDLTLEPPAADPSVLDLPSVRKALARYQLGVVIHTAYYLP
                     YAVPFRSVRAACREEFSHALRAAHQIGAELVTIHFSRPPRFFTDDQVVEWHRDIVDPL
                     CAMAENWGIRIALEQIPFPNCDQMGIIQRLLNALPNLGFHLDSGHAQLERKGDLWYEY
                     LERFGARLCHVHLSENDGTSDQHLPLGSVPHVGLDWPDRIARLKAIPYDGTITLEIFS
                     AEPEYLLQSRNLLRRWWDS"
BASE COUNT          394 a          443 c          386 g          342 t
ORIGIN      
        1 gagctctttt gtgagctgag tgatcccccc ttatccagtg tggcacttaa cgcggaagcc
       61 gccgggtatc gagcggcggc acttctggat cggatgatgc taggagagaa gattgccccc
      121 caagaaatcg tcgcggaggc ccttcacgtc gttacacgaa ggtcgagtga gattgtggcc
      181 atggaagacc ctgatcttac ggcggccctg cagttcattc atgaccacgt gacagaacca
      241 attacggtgg ccgacgttgt gtcggccgtt cacgtctccc ggcgtgccct tgagttacgt
      301 tttcgtcgac agttgggccg aaccatccac gaggaactcg aacgactacg tctccaacgg
      361 gcagagcgac tcctcatcga gacggagctg cccatcccat acgttgctga agccgttgga
      421 tacaattcac ccaactattt cgcccgaatc tttcaacaaa aacgcttgat gagtcctaca
      481 gaatttcgcc ggcaaaaaag aggagaactc ctcagacaag accttcgcgt ctggaaaacg
      541 atgatcgccg aatgagacac tctcctaata atcttttctt ttcaagaatc ccaccaacga
      601 cgaagcaaat tacggctttg aagaagatac tctggttcgg cagagaagat ttccagggta
      661 atcgtgccgt cataggggat cgccttcaat cgggcgattc gatcaggcca atccagcccc
      721 acatgcggca cggaccccaa cggtaaatgc tggtctgatg tcccatcgtt ttcggagagg
      781 tgaacatgac agagccgtgc accaaatcgc tcaaggtact cgtaccacag gtcacccttt
      841 ctttctagtt gtgcatggcc gctgtccaaa tgaaaaccga ggtttggcaa ggcattcaag
      901 agcctttgaa taatacccat ctggtcacaa ttgggaaaag gaatctgctc caaggcgatc
      961 cggatgcccc aattttcggc catcgcgcaa aggggatcaa cgatgtcgcg atgccactcc
     1021 accacttggt cgtccgtaaa aaagcggggt ggtcgagaaa aatggatcgt cacaagctca
     1081 gccccaattt gatgcgccgc ccgcagggca tggctaaact cctcgcggca cgccgcacga
     1141 acagaccgaa acggaacggc atacggcaag taataggccg tgtggattac cacgcctaat
     1201 tgataacgtg ccaaggcctt ccgtacactc ggcaagtcga gaaccgatgg gtccgcggcc
     1261 ggtggttcca acgtcagatc gacaaagtca aatccgtttt ctgcgatcca gcgaatctct
     1321 tcgataacat ctttcgccgg atggttcatg gcaccaatcc gcatcgccat tccctcaatc
     1381 aattctcgaa tttgccacgt ccattttgac tcgccaaggt cttggtcatg cttcattctc
     1441 agacaaaaaa gaaagggcac gaacaccatg attcgtaccc tatgtcactc atcatccgtc
     1501 aatgacgctg gtctcacgtg acggagaccc cgcatttatt cccggcgatt aggcataccg
     1561 gttaa
//
LOCUS       DAOPTR010000999         1564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_62215_length_1564_cov_2.465209, whole genome shotgun sequence.
ACCESSION   DAOPTR010000999 DAOPTR010000000
VERSION     DAOPTR010000999.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62215_length_1564_cov_2.465209"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="PKI05_15270"
     CDS             <1..644
                     /locus_tag="PKI05_15270"
                     /inference="COORDINATES: protein motif:HMM:NF014972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOA53614.1"
                     /translation="GCRAAIADAGGRILGQGDLMTLIEQAQQKIDQEEMRRQQERLQQ
                     GQFTLEDFRNLLDQMRRLGPINQMMSLIPGMSQLVNEMDDVDAEHSLRRLRGIIDSMT
                     PEERRKPEIIDLSRRRRIAAGAGVAPHDVAELVRVFEPIADIMRKLAGQGMRGRVQML
                     QTLMRQAATNPEAMLSKPKVGTGKRLRPEEKARLKKLREKELRRRRKEQRRGG"
     gene            complement(784..>1564)
                     /locus_tag="PKI05_15275"
     CDS             complement(784..>1564)
                     /locus_tag="PKI05_15275"
                     /inference="COORDINATES: protein motif:HMM:NF022135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HOA53615.1"
                     /translation="TWAVGASAHQIWIEAPSTAAANVPVALEVCFGHSGERSTGPILV
                     DNRPKLAAVVKTPDGQEEALSLGLNEDGYPAQFQARQAGFYHLGAVLETGIIERELHQ
                     IPPKTRIVMMGKAIVAVGEAREGLSLAVGHPLEILPVTNPCGLRVGDKITLRIVYKGK
                     PIGGPDVLVRVGTLGPNPKDDPQLTEREWAIEGYPDSRGEVSFPLIAAGQHVVFLRYM
                     DETPGEYAGTLSFSSDFSHLQPGDRYERTLYMTTLTFQVQP"
BASE COUNT          342 a          486 c          428 g          308 t
ORIGIN      
        1 ctggctgccg cgccgcgatc gcggatgccg ggggccggat tctgggccag ggcgatctga
       61 tgacactgat tgaacaggcg cagcaaaaga tcgatcagga ggaaatgcgg cgccaacaag
      121 agcgcttgca gcaagggcaa ttcaccctcg aggacttccg caacctcctc gatcagatgc
      181 gtcgcctcgg tcctatcaac cagatgatgt cactcatccc cggcatgagc cagttggtca
      241 acgaaatgga cgacgttgat gccgaacact ccttgcgacg ccttcggggt atcattgatt
      301 ccatgacgcc cgaggaacgc cgcaagccgg agatcatcga cctgagtcgg cgtcgccgga
      361 tcgctgccgg ggcgggtgtc gcccctcatg acgtggcaga gcttgtccgg gtctttgagc
      421 ccattgccga catcatgcga aaactggccg gtcaagggat gcgtggacgc gtgcaaatgc
      481 tgcaaacgct catgcgacag gccgctacga atccggaagc gatgctctcc aagccaaaag
      541 tcggcacggg aaaacgcctg cgtcccgaag agaaggcccg actgaaaaag ctccgtgaaa
      601 aagaactccg ccgacgacgc aaggaacagc gccgcggcgg ataagccgcc ctctttctga
      661 ttctttcttc aatcacaaat cccacacacc gtgggccgcg tttcgtggcc ccttgcccct
      721 ctcctgactt tttgcttttg ccaagatgat ggcacgcttc ccctgggtgt tcggccgttc
      781 ccctcaaggt tgaacctgga aagtcagagt cgtcatataa agagtccgct cgtaccggtc
      841 gcctggctgc aagtgactga aatcgctgga gaacgacaag gtccccgcat attccccagg
      901 cgtttcatcc atatagcgga gaaacaccac atgctggccc gccgcaatca gggggaacga
      961 gacctctccc cgcgaatcgg gatatccttc gatcgcccat tcccgctcag ttagttgcgg
     1021 gtcatcctta ggatttggtc ccaaagttcc cacacgaacg aggacatccg gtccgccgat
     1081 gggcttcccc ttatagacaa tccgcagggt gattttatct cccacacgaa gaccgcaagg
     1141 attggtaacg ggcaaaatct ccaggggatg gcccaccgcc agggaaaggc cttcgcgggc
     1201 ctcaccaacg gccacgatcg ccttgcccat catgacgatt ctcgtcttcg gcgggatttg
     1261 atgaagctcg cgctcaatga tcccagtttc gagtacagct ccaaggtgat aaaaccccgc
     1321 ttgtctggcc tggaactggg cggggtatcc atcctcattt aatccgagag ataaggcctc
     1381 ttcttgacca tcaggtgttt tgaccacggc cgccagcttc ggccgattgt ccaccaagat
     1441 cggtcctgtc gatctttcgc ccgaatggcc aaaacagacc tccagcgcga ccggaacatt
     1501 tgccgccgct gtggaagggg cctcaatcca gatctgatgt gcggatgctc ccaccgccca
     1561 ggtc
//
LOCUS       DAOPTR010001000         1561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_62348_length_1561_cov_4.143426, whole genome shotgun sequence.
ACCESSION   DAOPTR010001000 DAOPTR010000000
VERSION     DAOPTR010001000.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62348_length_1561_cov_4.143426"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..1544
                     /locus_tag="PKI05_15280"
     CDS             237..1544
                     /locus_tag="PKI05_15280"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA53616.1"
                     /translation="MSDVLKTSRRSFLETSAVVGGAAMLGSLSLARSVHAAGSDVMKV
                     CLVGCGGRGNGAVRDHIRAASEAGYKVQVVAVADAFEDRAKGAADSLRKEFGKDIVDL
                     PDDRVFVGLDSYKKAIDCGVDIVINASPPGFRPFQFAYAIQAGKHVFMEKPVCVDAPG
                     FRMVWEACKMADEKGLKVGVGLQRHHQASYIREIAAIKEGKIGDIMFLRAYWNGGAIW
                     IRRRREDQNELQYQVYNWYHFTWLSGDNIVEQHVHNLDVCNWVMSDDLTNPVHPVEAN
                     GMGGCEMRYRGENRGVGQIFDHHFVEFTYPNGVKMYSQCRQINNCWSNVSEAAHGTKG
                     VSGCQSGPGGNNPYVQEHIDLLNAIAKNERYNEGYFGATSSFTAVLGRMATYSGQVVR
                     WDEAVEKGPVIAPEITSWDQEPPVKKDENGDYPIAQPGVYKPY"
BASE COUNT          357 a          391 c          475 g          338 t
ORIGIN      
        1 accgaagagg aagccaaact gtatgaaccc gatccggaaa tccttctgaa ctatcgcaag
       61 gcgcagggcg gaaagcagtg acgtgaagtc gctggctccc gcatcagttg cgaacgacgt
      121 gcgaagggga taatctgtat gtcttgagaa gtcgagctgc ctcgtcggtc aaagacgaca
      181 gtggcggtaa gaacaacttg ggtttttgct agaacttttt gtaaggagcc tgcgtcatga
      241 gtgatgtgct aaagacatct cggagaagtt tcttggagac atcagcggtc gttggtgggg
      301 ccgctatgtt gggttcgctc agtctggcca ggtcggttca cgccgccggc agcgatgtca
      361 tgaaggtctg cttggtgggt tgcggtggcc ggggaaatgg cgcggtccgt gaccacatca
      421 gagccgccag tgaagcaggc tacaaggtgc aggttgtggc tgtggccgat gcctttgaag
      481 atcgtgccaa aggtgcagcc gacagccttc gtaaggaatt cgggaaagat attgtcgatt
      541 tgccggatga tcgagtcttt gtcggcttag attcatataa gaaagccatt gattgcggcg
      601 ttgatattgt catcaatgcc tcgccgccgg gattccgtcc tttccagttc gcctatgcta
      661 tccaggctgg caagcacgtg tttatggaaa agcccgtctg cgtggatgcc cctggtttcc
      721 ggatggtctg ggaggcttgc aaaatggcgg atgagaaggg cttgaaggtc ggcgtcgggc
      781 tccaacggca ccatcaggcc tcctacatcc gtgagatcgc tgccatcaaa gaaggcaaga
      841 tcggcgatat catgttcctc cgtgcctatt ggaacggcgg tgcgatttgg atccgacgcc
      901 gtcgggaaga tcaaaatgag cttcaatacc aggtgtataa ctggtatcac ttcacctggt
      961 tgagtggtga taacattgtg gagcagcacg tgcacaacct cgacgtttgc aactgggtga
     1021 tgagcgatga tctgacaaat cccgtgcatc ccgtcgaggc caacggtatg ggtggctgcg
     1081 aaatgcgcta tcgcggcgag aatcgcggcg ttggccaaat cttcgatcac cacttcgtgg
     1141 aatttactta tcccaacggc gtcaagatgt acagccagtg ccgtcaaatc aataactgct
     1201 ggagcaatgt gtcagaggca gcccacggga cgaagggcgt ctctgggtgc caaagcggcc
     1261 ccggcggcaa caacccgtat gtccaggaac acattgacct gcttaacgcc attgccaaga
     1321 acgaacgtta taacgagggg tacttcggcg cgaccagcag ctttactgca gtgctcggcc
     1381 gaatggccac ctacagcggc caggtggtcc gctgggacga ggccgtcgaa aaaggtccgg
     1441 tcattgcacc ggaaatcacc tcgtgggatc aagagccacc cgtcaagaag gacgagaacg
     1501 gcgattaccc gatcgcccag cccggtgtgt ataagccgta ctgagtcccc ttcgttgata
     1561 g
//
LOCUS       DAOPTR010001001         1561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_62352_length_1561_cov_3.682603, whole genome shotgun sequence.
ACCESSION   DAOPTR010001001 DAOPTR010000000
VERSION     DAOPTR010001001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62352_length_1561_cov_3.682603"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="PKI05_15285"
     CDS             complement(<1..1258)
                     /locus_tag="PKI05_15285"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOA53617.1"
                     /translation="MSTEQPLDPQLIEQTRQQIRQLVAEVAELSSRTDLTPDQYCAEF
                     LPRVVSALAAFGGVFWTVEGPGRISLTYQMNLQQTRLAEDEEARQKHNRLLQRVLQTR
                     EAILVPPQSGSGENNGEANPTDFLLLLGPVGTELEVVGVVEIFQRPDAPPAAQKGYLR
                     FLMQMCDMASDYFKSLQLRSLSDRQSLWSRLEEFTRLIHQSLDPAETAYTLANEARRL
                     IECDRVTISVRRGRRQVIEAISGQDVVNKRSNVVRLLGDLATVVATARENVWFCGDTS
                     QLAPQIEDALQQYVDESHSKTVIVIPLGRPAPPPSDEAEADRPADPPPPVGTMIVEQI
                     EDSRITPQLRQRVEVVAQHGAVALANAVEHNEIFLMPLWKAIGRSKVITEARMLPKTA
                     AVGIVIVLLLLTLLFAPWPYKLHARGT"
BASE COUNT          316 a          472 c          453 g          320 t
ORIGIN      
        1 gtgttccccg ggcatgaagc ttatacggcc agggagcaaa aagcagagtc aaaagaagga
       61 ggacgatgac gattcccacg gccgccgtct tgggcaacat gcgggcttcg gtgatgacct
      121 tagatcgccc gatcgccttc cacagcggca tcaggaaaat ctcgttatgc tccacggcat
      181 tggccaaggc cacggcaccg tgttgggcca ccacctccac ccgctgccgt agctgcggcg
      241 tgatacggct gtcctcgatc tgttcgacaa tcatcgtgcc caccggaggc ggggggtccg
      301 ccggtcgatc ggcctcggct tcgtccgatg ggggaggtgc cggacgaccc agcgggatga
      361 caatcaccgt cttggaatgc gattcatcga cgtactgctg gagagcatcc tcgatctgtg
      421 gtgccaattg cgatgtgtcg ccgcagaacc aaacgttctc ccgcgctgtc gcgacgaccg
      481 tcgccaaatc tcccaacaga cgcaccacgt tggaccgctt gttgactacg tcctgccccg
      541 agatggcctc aatgacttgc cgacgcccac gtcgcacaga aatcgtgact cggtcgcatt
      601 cgatcaagcg gcgcgcttcg ttggccagcg tgtaggctgt ctctgccgga tcaaggctct
      661 gatgaatgag ccgcgtaaac tcttccaatc gcgaccatag gctttgccga tccgacagac
      721 tgcgcaactg cagcgatttg aaataatcgc tggccatgtc gcacatctgc atgagaaagc
      781 ggagataacc cttctgggct gcgggcgggg catcgggacg ttggaaaatc tccacaaccc
      841 ccacgacttc cagctccgtc cccaccgggc ccagaagtaa gaggaaatcc gtagggttgg
      901 cctcgccgtt gttttctccg ctgcctgatt gcggcggtac caggatcgct tcccgcgttt
      961 gtagtacccg ttggagcagg cggttgtgtt tttgtcgggc ctcctcgtct tccgccagtc
     1021 gagtctgctg gaggttcatc tgataagtca ggctaatccg cccggggcct tcgactgtcc
     1081 aaaagacgcc cccaaaggcg gcaagggccg acacaacccg cggcaaaaac tccgcgcagt
     1141 attgatcggg cgtcagatcg gttcgggagg aaagctcggc cacctccgcc accaattgcc
     1201 ggatttgctg ccgcgtctgt tcgatcagtt gcggatcgag aggttgttcg gtgctcatgc
     1261 cgcgctgcta tctagtgcgc tggttcgtat cctgggtcag aaaacgatac cttctgacct
     1321 tttgccgcca tccccccaat cctattgtag cggattaccg cacattgcag gcgattcctg
     1381 cggaaatggg attgggaagg gaggcccttg tcaagactat accttacaaa tcgatcgggc
     1441 tcggtgagac gatcctgggc gaaaggggca gccgaagcag gcgaagggct aaaacgccaa
     1501 tccgccactt aagaagaccg cagcgtcggg cttgtactta tcggtccaac cggcctcgta
     1561 a
//
LOCUS       DAOPTR010001002         1559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_62466_length_1559_cov_2.919548, whole genome shotgun sequence.
ACCESSION   DAOPTR010001002 DAOPTR010000000
VERSION     DAOPTR010001002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62466_length_1559_cov_2.919548"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="PKI05_15290"
     CDS             complement(<1..855)
                     /locus_tag="PKI05_15290"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOA53618.1"
                     /translation="MGYSASFLEPQVLAGINGLVLRARGIVEGHVTGLHRSPFHGFSV
                     EFAEHREYSPGDDLRYVDWKVFGRTDKFYIKQFEQETNLSCYLLVDSSESMKFQSKGS
                     PLSKWAYAQCAAAALAYILLNQQDRVGLTIFSEKVLTHLKPEAGMPQWSNIISVLEQF
                     QPLGKTGLGGVLSEIAERAKQRGMVILLSDFFTDLSEVEKGLKHLKFQRHDVIVFQIV
                     DRQEVTFDIEKPVVLRGLEGGATLAVDPAWLRRAYQTRFRQFCLQLQKICHDSGVDYV
                     FVETINRFD"
     gene            complement(830..>1559)
                     /locus_tag="PKI05_15295"
     CDS             complement(830..>1559)
                     /locus_tag="PKI05_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOA53619.1"
                     /translation="LRFLPGPIFANLVLADEINRTPPKTQAALLEAMQEYQVTVGGKR
                     HPLEPPFYVLATQNPIEQEGTYPLPEAQLDRFMMNIQIDYPTEDEEMDIVLRTTSDIS
                     MTVESVLTGEDVLKLQRVVRRIAVPEHVARYAVKITRWSRPQVPDGPKFVKDYVTWGG
                     GPRATQCLVLAAKARAALHARPAIDIEDIRAVVLPVLRHRIILSFNAVAEGLKPDDII
                     CRLLDSIGNPEDALIARGILRKLS"
BASE COUNT          375 a          438 c          393 g          353 t
ORIGIN      
        1 gtcaaatctg ttgatcgttt ccacaaacac ataatccacg ccgctatcgt gacatatctt
       61 ctgcaactga aggcaaaatt gacgaaacct tgtttggtac gcacggcgta accatgccgg
      121 atcaaccgcc agcgttgcgc cgccttcgag ccctcttaac acgacaggtt tctcgatatc
      181 aaacgtcacc tcctggcgat cgacaatctg aaaaacgatg acatcgtgcc gctggaattt
      241 gagatgcttc agtcccttct caacctcgga aagatctgtg aagaaatcgc tgagcagaat
      301 caccatgccc cgctgcttgg cccgttctgc aatctcgctt aaaacaccac ccaatcccgt
      361 cttacctaat ggctggaatt gctcaagaac gctaatgata ttgctccatt ggggcatgcc
      421 ggcctcaggc ttgaggtggg tgaggacctt ctcggaaaag atggtcagac cgactcgatc
      481 ttgctgattt agcaagatgt acgccaaagc agccgccgca cactgggcat atgcccactt
      541 gctgagtggg ctacctttgc tttggaactt catgctctcg ctggagtcca ccagaagata
      601 acacgagagg ttggtctcct gttcaaattg tttgatgtaa aacttgtcgg ttcgaccaaa
      661 gaccttccaa tccacatagc gaaggtcatc gcccggtgaa tattcgcggt gttcggcaaa
      721 ctccaccgaa aacccatgaa acggactacg gtgcaagccc gtgacgtgcc cctcaactat
      781 gccacgggca cgaaggacaa gtccgttgat gccagccaag acttgtggtt caagaaagct
      841 tgcggagtat cccacgggcg atcaaagcat cctcagggtt accgatggaa tccaggagac
      901 ggcaaatgat atcgtctggt ttcagacctt ctgccacggc attgaaactt aggatgatcc
      961 gatgccgtag tactggcagg actacggccc gtatgtcctc aatatcaatg gcgggacgcg
     1021 catgcaaggc ggctcgtgcc ttggcggcca aaaccaggca ttgcgtggcc cgcggccccc
     1081 caccccaggt gacataatct ttcacgaatt tcggaccatc aggaacttgc ggacgactcc
     1141 agcgcgtgat tttgacggca tatcgggcaa catgctccgg caccgcgatg cgacgcacca
     1201 ctcgctgaag tttgagcaca tcttcgcccg tcaaaaccga ctctaccgtc atggatatgt
     1261 ccgacgtggt acgcaggacg atatccattt cttcatcttc cgtgggatag tcgatctgga
     1321 tattcatcat gaaccggtcc agttgggcct ctggaagggg ataggttcct tcctgctcga
     1381 tcggattttg agtcgctagg acataaaaag gcggttctaa ggggtgacgc ttgcccccca
     1441 ccgtgacttg atattcctgc atcgcctcga gcaatgccgc ctgggtcttg ggaggcgtac
     1501 gattgatctc gtcggccagc accaaattgg caaaaatagg gcccggcaga aatcgcaat
//
LOCUS       DAOPTR010001003         1555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_62673_length_1555_cov_3.628667, whole genome shotgun sequence.
ACCESSION   DAOPTR010001003 DAOPTR010000000
VERSION     DAOPTR010001003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62673_length_1555_cov_3.628667"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PKI05_15300"
     CDS             complement(<1..625)
                     /locus_tag="PKI05_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53620.1"
                     /translation="MLRSVITSLVFLVIYLNLAIGYGDGLSTTLVANGRQRAIPRPFE
                     HHPGNVFLEGEPIRISLPVAGSWSLYDAEDRLLKEVRTDGHEIDLGPLPVGFYRLRNP
                     QVSDWVSLAVIAPLQAPTPETSPICIDVAMSWFYPENKMEDVASLCALAGVNWVRDRL
                     SWGEVEPQRGEFVAKTKYDTAATTQAAAGLKVLQVFHSSPRWANPNGK"
     gene            complement(700..>1555)
                     /locus_tag="PKI05_15305"
     CDS             complement(700..>1555)
                     /locus_tag="PKI05_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53621.1"
                     /translation="LPTAEEISQDFHEDLRGLQEVQAVELKKMRFEHNLVPLAARQPA
                     LVAEWQKVAPSNVAVESGVFVFRADKTEALPLEYRPYDTGHTIDCNWKLCRWPDISHP
                     LVNGHVKAAKGEKGPVEINVPGPGLFAFDPGTAYWRAAEIGFDHRPLSLWAGRPKDDT
                     KGFPPLRLWRPRLDQPLYLFVPKGTSDLVIGIVEGGDPYTVIQVKTSKGTVLCQERLL
                     AGDQLCVSVNPQARDRDDPTDEDKAIRGGKVLSVGFDSLRCVIEIYNVPPYLARHPSE
                     LLVPVDAM"
BASE COUNT          357 a          430 c          410 g          358 t
ORIGIN      
        1 gtttgccgtt tggattggcc caccgtggcg agctatgaaa gacctgcaac actttcaagc
       61 ccgctgctgc ctgggtggtt gctgccgtgt cgtatttcgt tttggccacg aattcccctc
      121 gttgtggctc aacttctccc cacgacaaac gatctcgaac ccagttaact cccgctagcg
      181 cacataaact tgctacgtcc tccattttgt tttctggata aaaccaactc atcgcgacat
      241 caatgcaaat gggagaagtt tcgggcgtgg gcgcttgcag gggggcaatt acagccaacg
      301 aaacccagtc gctcacctgg gggtttcgaa gtcgatagaa tcccacaggg agaggcccta
      361 aatcgatctc gtgtccgtcc gtgcggactt ctttgaggag ccgatcttcg gcgtcgtaca
      421 acgaccagga acccgcgacg ggaagagaaa tgcgaatcgg ctcaccctct agaaaaacgt
      481 tgcctgggtg gtgctcaaat ggccgcggga tagcacgttg acggccgttc gcgacaagcg
      541 tggttgaaag tccgtctccg tatccgatag caagatttaa ataaattact agaaacacaa
      601 gcgatgttat tacgctgcgg agcatgggtc acctcatagg ttggcgagag actcgtaaac
      661 ctttgttagc tgcccttctc aaccaacata acaatagctt cacatagcat cgaccggtac
      721 caagagctcc gaaggatggc gcgccaggta cgggggaacg ttgtaaattt cgatgacgca
      781 gcggagcgaa tcaaacccaa ccgagagaac cttccctcct cggatggcct tgtcctcatc
      841 ggtcgggtcg tctctatccc gcgcttgtgg attgacactc acgcaaagct ggtcaccagc
      901 taagagccgc tcctgacaaa ggacagttcc tttgctggtc ttgacttgga tgaccgtgta
      961 tgggtctccg ccttccacga tgccgattac cagatcggac gttcctttgg ggacgaacaa
     1021 gtacaacggc tgatcgagac gcggccgcca cagccgcaaa ggcggaaaac cttttgtgtc
     1081 gtctttggga cgcccggccc agagagatag cggtctgtga tcaaagccaa tctccgcagc
     1141 acgccagtaa gctgttcccg gatcaaaggc gaacaagccc ggtccgggca cgtttatctc
     1201 gacaggtcct ttttcaccct tggctgcctt gacgtgacca ttgacgaggg ggtgactaat
     1261 gtctggccag cgacagagct tccaattgca gtcaatggtg tgtccggtgt cataggggcg
     1321 atattcgaga ggtaatgcct ctgttttatc tgcacgaaag acgaacactc ctgattccac
     1381 agcaacgttt gacggggcaa ccttctgcca ttctgccacc aatgctggtt gacgtgctgc
     1441 caatgggaca aggttgtgct cgaatctcat ctttttgagc tcaaccgcct gaacttcctg
     1501 aagtccccgc aaatcttcat ggaaatcctg ggagatttct tcggcagtcg gcaac
//
LOCUS       DAOPTR010001004         1555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_62686_length_1555_cov_2.783333, whole genome shotgun sequence.
ACCESSION   DAOPTR010001004 DAOPTR010000000
VERSION     DAOPTR010001004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62686_length_1555_cov_2.783333"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1538)
                     /locus_tag="PKI05_15310"
     CDS             complement(<1..1538)
                     /locus_tag="PKI05_15310"
                     /inference="COORDINATES: protein motif:HMM:NF013400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53622.1"
                     /translation="MRRSFVKTCFPIVPGFLALWLVVQWTSLSIEARGHEQPNPSDSK
                     FIGLDVQTITNFGGRVAGTVRSQEISGESFVFLGSEVMLVLPAERLLTPEQGSISFTV
                     IATWPGNDQRAHTFVHMAIGPCHVTIFKTDTNTLRFTYRGREEVHAACDLDITDWQPN
                     EPHEISAGWCKGYGDLLWIVLRVDGREALTHRAVPLVTLPEELFIGRRGPSAQPAEAW
                     IGKLQIRPVAPVLPFAAGPKPPVMAEVDCSVTQPFRRVHDFTTIWNSQENPVPFGVGS
                     PEFRLFQQGGFRMARLVAFSEGWLWGTRVTRDETGRLVTDFSDFDRLLSVITAAGAEP
                     YIRLAYHTPSALVSPSTLAEHRRYALPENLELWDELMERIVRHVLFEQKLPVRYWVTA
                     LNEGDLPVARGQAEPETIYQLYERTSRIVKRLDPQAKVGGPALASSIDLEGRPMPMFV
                     DFLRYCRDRDVPLDFVCFHGYGFAHPRQYEGLVAGVRSAVQTVWPEKAPSVELFLDEW
                     NLWSR"
BASE COUNT          368 a          448 c          442 g          297 t
ORIGIN      
        1 cgggaccaga ggttccattc atcaaggaaa agttcgacgg agggtgcctt ctccggccac
       61 acagtttgca cggcactgcg gacaccggcg accaggccct catattggcg aggatgggca
      121 aaaccatagc cgtggaagca aacaaagtcg aggggcacgt ctcgatcacg acaatagcga
      181 aggaaatcca cgaacatcgg catgggtcgg ccttcgagat cgatcgagct agcgagggcc
      241 ggtccgccca ccttggcctg cggatcgagc cgtttcacaa tccgcgaagt ccgctcgtag
      301 agttggtaga tggtctccgg ctcggcctgc ccgcgggcga ccggaagatc accctcgttc
      361 aacgccgtta cccaataacg gacgggcagc ttttgctcga aaagcacatg tcggacaata
      421 cgttccatca attcatccca aagctccaag ttctcgggca aagcgtagcg acggtgttcg
      481 gccaaggtgc tcggggacac caaggccgac ggggtgtgat acgccaaccg gatgtatggt
      541 tcggcccccg cagcagtgat cacgctgagc aagcggtcaa agtcgctaaa gtcagtgacc
      601 aagcggcctg tctcatcgcg ggtcactcgc gtgccccaca gccagccctc agaaaaggcc
      661 accaaacggg ccatgcggaa gccaccttgc tggaatagac gaaactctgg agagccaacc
      721 ccgaatggta ccgggttctc ttggctattc caaatggtcg taaaatcgtg aacacgtcta
      781 aaaggttgtg tcacggaaca atcgacctct gccataactg gcggtttcgg gccggcggca
      841 aacggcaaca ctggggcaac agggcgaatt tgtagcttgc ctatccaggc ctcggccggt
      901 tgcgcactgg ggccacgccg cccgatgaaa agctcctcgg gtaacgtcac gagaggaaca
      961 gcccggtgcg tcaaggcttc ccgcccatcg acgcgcagga cgatccacag cagatctccg
     1021 taacctttac accagccggc tgaaatctcg tgcggctcat tgggttgcca atctgtaatg
     1081 tccaggtcac aggctgcgtg gacctcctcg cgaccacggt aggtaaagcg gagcgtattc
     1141 gtgtcggtct tgaaaatcgt cacatgacag ggaccgatgg ccatgtgtac gaacgtgtga
     1201 gcacgctgat catttcctgg ccaagttgcg atgacagtaa acgaaatgct cccttgttct
     1261 ggtgtcagga gccgctctgc cgggaggacg agcatcacct ccgaaccgag aaaaacaaac
     1321 gattctccac ttatttcttg gcttcggact gtccctgcca cgcgaccacc gaaattggtt
     1381 attgtttgca catcgagtcc aatgaacttc gagtcggagg gattaggttg ttcatgccca
     1441 cgggcctcaa tggacaagga tgtccattgc acgacaagcc acagcgcgag gaatcctggc
     1501 acaatgggga aacaggtttt aacaaacgac cttcgcatgt tggaggctcc ttgta
//
LOCUS       DAOPTR010001005         1554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_62756_length_1554_cov_2.508339, whole genome shotgun sequence.
ACCESSION   DAOPTR010001005 DAOPTR010000000
VERSION     DAOPTR010001005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62756_length_1554_cov_2.508339"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PKI05_15315"
     CDS             complement(<1..798)
                     /locus_tag="PKI05_15315"
                     /inference="COORDINATES: protein motif:HMM:NF014097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HOA53623.1"
                     /translation="MSKSSSQAGVFRTPYRREDRKHRHRMEILTVLRKSEQPLSSSDI
                     AQMLAAVGYMLSERSIRLYLAEMCEEGLTISHGHRGHTISEKGLAELRVAQTIQRVGY
                     LSARIDEMTYKMTFDLERRAGTVVVNTSVVNPHDLAVCLDSVCQVFTKGFAMGSRLAL
                     LEPGERIGELQIPPGRIGFCTICSITLNSVLLKHGVPTTSRFGGLLEVRDGKPLRFAE
                     IIHYDGTSIDPLEVFIRGRMTDYRGAIATGNGLIGASFREIPEDARDL"
BASE COUNT          340 a          442 c          423 g          349 t
ORIGIN      
        1 cagatcgcgg gcatcttcgg gaatttcccg aaaactggcc ccgatcagtc cgttgccggt
       61 ggcaatggcg ccacgatagt cggtcatccg tccgcggatg aagacttcca gaggatcgat
      121 actggtccca tcgtagtgaa tgatctcggc aaaacgcaaa ggtttaccat cgcggacttc
      181 tagtaagccg ccgaagcgag aggttgtggg aacaccatgt tttaagagga cactgttgag
      241 ggtaatcgaa caaatggtac aaaacccaat tcggccaggc ggaatctgta gctcaccaat
      301 ccgctcgccg ggctctagca gtgccaagcg gctgcccata gcgaagcctt tggtgaacac
      361 ctggcaaacg ctatccaggc aaacagcaag atcatgggga ttgaccacag aggtattgac
      421 aaccacggtg cccgcgcggc gctccaggtc aaaggtcatt ttgtaggtca tttcatcaat
      481 tcgggcggat agataaccga cacgctggat ggtctgcgcc acacgcagct cagccaaacc
      541 cttttcagag atcgtatggc cacggtggcc gtgagagatg gttagccctt cttcgcacat
      601 ctcggcaaga tagagccgaa tgctccgttc gctgagcata tatcccacag cggctagcat
      661 ttgagcgatg tcgctggaag ataatggctg ctcggatttt cgcaggacgg tcagaatctc
      721 catccggtgg cggtgcttgc ggtcctccct tcgataaggg gtacggaata cccctgcttg
      781 cgaactcgac ttactcaccc tcatcaccgt gacctcactt tggatgggat atgtgtcgcc
      841 ttggcagctc cgttgggccg tgccccatat cggcattctt tcccgttggc tcgggcttcc
      901 ccggcaaggc cgacgcgccc tctaccgaaa catggccgac tttcggcaaa aaaatcaagt
      961 tctcggatgt ttggttgccg acttttccgg atgactggca attttgccga cttcaatcca
     1021 gcttgtcaag cggggttaca tggagatcgt cctccggcgt gcgctccgcg gtcggatgca
     1081 tggacgtcgc gcaccgcgag aacgattcga gcgcgatccg ctccgtacgg cttttaggcc
     1141 agtttttccg catctacgtt gcagggggct tgccgtcatc ctcggaggat cgcccacggc
     1201 acacaagcag ccctcagcga gaaaacgatc cccgaagccc ttgaactcgt cggctatttg
     1261 gaatagatta gacaatattc tggcgacaga ttgccgaatc tttggtcatg agttacccag
     1321 ctttcggttg ttgctcgctt ggttatcccc cccgttcgaa gccatggctc gggcctcgcc
     1381 gcaacgaggt actctcatgc ccacgaaccg tacgtttgat ccgttctatg atcaggagtg
     1441 cacacgatga aactcacctc aaccgtcgcc gctaatgtcg agaagttcat ggagtgggtt
     1501 gtcaagagga accccaatga accggaattt catcaagccg ttcgggaagt cgcg
//
LOCUS       DAOPTR010001006         1553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_62825_length_1553_cov_2.285714, whole genome shotgun sequence.
ACCESSION   DAOPTR010001006 DAOPTR010000000
VERSION     DAOPTR010001006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62825_length_1553_cov_2.285714"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..>1553)
                     /locus_tag="PKI05_15320"
     CDS             complement(2..>1553)
                     /locus_tag="PKI05_15320"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOA53624.1"
                     /translation="PPRNRNNKQLDRFEVTVVGYVPIDYPTNYYRFDGPEWHRKIPQQ
                     SPGSDGWPGWWPDPLLPKSQFSLAAETTQAVWIVFKIPQDAESGDYDGTVRFIGQGRI
                     LAEVPFSVHIWNFALPRETTFPAIYDVRLGPAGRVFGQTLDQLYPEIVRFMAERRLCP
                     DKIRPDPVIEYRDGQVRADFTEYDRVARWYFDTLKLPVTYTPHVFYLFGWGFPPRNFF
                     GQTPYPGDSPFEGVNRSELRPEYVAAYQACLKCYWDHMKENGWSDRVVLYISDEPFDH
                     LPEIREQMIALCKMIHDAVPDIPIYSSTWHHIPDWDGSITVWGLGHDGRVPSEKLRAL
                     REGGAQVWFTTDGQMCTDTPYCAVERLLPHYCFHHGVGAYEFWGVAWTTYDPYRFGWH
                     SFIPQSDRPGNRYWVRYPNGDGFLIYPGKPIGYDGLVSSIRLEQAREGVEDYEYLSLL
                     KTAATQAPPDDPRRKAAESVLAKASQLATIPNAGGRYSTKILPDPGLLEEIRKEIAET
                     IEQFVSNK"
BASE COUNT          385 a          417 c          400 g          351 t
ORIGIN      
        1 cttacttatt ggaaacaaac tgttcaatgg tttcggcaat ctctttgcgg atttcctcaa
       61 gtaacccagg atcaggaaga atctttgtcg aataacgtcc gcccgcatta ggtatggtcg
      121 ctaactggga ggcttttgcc aaaacactct cggccgcttt acgtctcgga tcatcgggag
      181 gtgcctgcgt tgcggcggtc ttgaggaggc tcaagtactc gtaatcttcg acgccttccc
      241 tggcctgctc cagccgaatg gaactgacga ggccgtcgta cccaattggt ttgcccggat
      301 agatcagaaa accatcgcca ttcgggtacc gaacccaata tctattacca ggtcggtcgc
      361 tttggggaat gaaggaatgc catccgaacc ggtaggggtc ataagttgtc cacgctaccc
      421 cccaaaactc gtatgccccg acgccgtggt ggaaacagta atggggaagc aatcgttcca
      481 cagcacagta cggtgtatcg gtgcacattt gaccgtcagt ggtaaaccat acctgggcac
      541 ctccttcccg gagggcgcgg agtttctccg aagggacgcg gccatcgtgt cccaatcccc
      601 aaacggtgat gctaccatcc caatcgggga tatggtgcca agtgctcgag tagatcggga
      661 tatccggaac agcatcatga atcatcttgc aaagggcaat catttgttct cgaatctcgg
      721 gaaggtggtc aaaaggttca tcggaaatat aaagaacgac acgatccgac cagccattct
      781 ctttcatgtg gtcccaataa catttgagac acgcttgata cgccgccacg tattcgggcc
      841 ggagctcgct gcgattgaca ccttcgaagg gagaatcgcc aggatacggc gtttggccaa
      901 aaaagttccg cggtgggaat ccccaaccga acaggtaaaa cacgtgaggt gtgtacgtta
      961 ccggtagctt aagagtatca aaataccacc ttgccactct gtcgtattcg gtgaaatcgg
     1021 cacgcacttg gccatcacga tactcaatga cagggtcagg tcggatctta tccggacaca
     1081 aacggcgttc cgccatgaaa cgcacaatct ctggatacaa ctggtccaac gtttgtccaa
     1141 aaactcgtcc ggccggtccc aatcgaacat cgtaaattgc cgggaatgtc gtctctcgcg
     1201 gcagtgcaaa attccaaatg tggacagaaa atggaacctc ggccaagata cgcccttgcc
     1261 cgatgaaccg gacagttccg tcatagtctc cagattcggc atcttgtggg atcttgaaga
     1321 cgatccacac ggcctgagtt gtctctgcgg ccaaggagaa ctgcgatttg ggcaaaagcg
     1381 gatctggcca ccagccaggc cacccatcac tgccgggcga ttgttgtgga atcttgcggt
     1441 gccattcggg tccatcaaac cggtaatagt tcgttggata atcgatgggg acataaccaa
     1501 ccacggtcac ctcaaatcgg tccaactgtt tgttattccg atttctagga ggc
//
LOCUS       DAOPTR010001007         1543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_63395_length_1543_cov_2.156586, whole genome shotgun sequence.
ACCESSION   DAOPTR010001007 DAOPTR010000000
VERSION     DAOPTR010001007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63395_length_1543_cov_2.156586"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..1303
                     /locus_tag="PKI05_15325"
                     /pseudo
     CDS             161..1303
                     /locus_tag="PKI05_15325"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
BASE COUNT          338 a          396 c          439 g          370 t
ORIGIN      
        1 tacgtcaaag aaactataat cagcgcatcg gagtttgtaa gccgctccga tagcgtcagt
       61 ttaagaaatt gctttttggt gttcaagcat gtctcgtaat tagttcgatg attgggtgct
      121 tttggtggtt caattttttt ccacagagta ggttgatgcg atgaaaaggt atggtgtgcc
      181 gaaaggtggg gagagaaggc aacgggcctt cacgcttgtc gagctgttgg tggtaattgc
      241 tattatcggc atattgatcg cgctcctcct tccggccatt caggcagctc gtgaggcagc
      301 gaggcgatca caatgtacca acaatctgaa gcaaattgga ctggctctgc ataatttcca
      361 tgatacatat gggcgttttc ccgcggctaa tcatgagaag aattttgcgg tccccgcggg
      421 gtcgggcgat tatcccaatt cggcccatcg ttgggggttc accgtgccct tgttgcctta
      481 catcgagcaa aaggccctct acgatgacat gatccaaaac tatattggga agacggatcc
      541 caacgggaat tcttattgcc cctggtacgg gtgtccccca acgcgcactt ggattgctac
      601 attgatttgt ccctctgact cgcgggccag ggaagttctc acatggaatg acggaaagac
      661 ccccactagc tacaagtgca accggggtga tcagtggctc aattgggact ggtgggaaac
      721 ccgtggtgtc tttggtaatg gctccaaggt ggaagtaaca atcggtacaa tcaccgacgg
      781 caccagcaat acgatcgcgg tatccgaggc cgtcatcggt attcaaggga gccgcaaggt
      841 gacctaagcc atcgccgtta acgtaggtgc ctataatagt gctccacctt cattgtgcct
      901 caaccaagtt ggtgccaatc gcaccttctg gggtgacgtt caaggttcgg attggcaggt
      961 tggctggcgg tgggccgacg gcattaatcc ctataccttg ttctatccca tgttgcctcc
     1021 gaacggtcca agctgcggca acagcgggga agactggtgc atcgtgacgg ccagcagccg
     1081 ccacccgggc ggggtcaacg tcctgttcgt ggacggggcc gtcaagttta tcagtgaaac
     1141 gatcgacgcg ggtgatccca cacgttcggc ctcggccttg ccgccgggtt atccgccacc
     1201 atcggatccc aacaggccac aggactatag gggcccatca ctctatggcg tttggggtgc
     1261 tttgggtagt gcctggggtg gtgagtcagt tccagcattg tgatgggtga cagcggaagg
     1321 cttaacgcga cgagcaacgt agtgcttggc taacactcgc gcgagcctac accagggccg
     1381 gttgtgtgat tttgcccata caaccaggcc ttggctattt tttgtttaac accgcggcaa
     1441 aaagcggcga aaaactcctc tctagcgggg cgtttggcca gccgtcacac tactggtacc
     1501 accccactta aaggagcggg atattcctat ccgttaccga gac
//
LOCUS       DAOPTR010001008         1539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_63614_length_1539_cov_4.404987, whole genome shotgun sequence.
ACCESSION   DAOPTR010001008 DAOPTR010000000
VERSION     DAOPTR010001008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63614_length_1539_cov_4.404987"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PKI05_15330"
     CDS             <1..416
                     /locus_tag="PKI05_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53625.1"
                     /translation="PVFMNSEGNGPLRRAYCISRMNKPIDQRLIDLIRLIVGLLVSGD
                     YDAIESRSRGVRLNAEQMKEAVQTHGHTLQSLPETSYAELDVIRVIRATCPTWSITVP
                     LWTIEEGRSDLTLECTVIDNGLPELDIEIDNLHVL"
     gene            478..954
                     /gene="comJ"
                     /locus_tag="PKI05_15335"
     CDS             478..954
                     /gene="comJ"
                     /locus_tag="PKI05_15335"
                     /inference="COORDINATES: protein motif:HMM:NF022486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComJ"
                     /protein_id="HOA53626.1"
                     /translation="MTTTSMAITVSYSQIAVFSSHLENPFNNWQEHHVTQGFSWREHS
                     VSFRTLNEFGSCSIEIDFSECHPGSDDAIRAIRVPFSVPEDGKIEIGSIGDSVPLSIA
                     PGAYALLFQILAVRPDGKEVIRLSFSKSEPMDFAIIKADEQITISGPLCKTSQAAT"
     gene            1041..>1539
                     /locus_tag="PKI05_15340"
     CDS             1041..>1539
                     /locus_tag="PKI05_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53627.1"
                     /translation="MMTAASDADLNHLFGIKKPFLAPLHLSTPLHTPRRLTPDEAAQI
                     CERDSAIKPTTRDNWELSPVSINGLLHAALSKMSNRGGRHAESTWHLPTAGVGQLRLS
                     SPLGSGRALTKDECRQLRSIDDRFAGANPAWTVKPSLSNPLQVDIVRYQNVNMWTVGQ
                     LSLAPV"
BASE COUNT          349 a          442 c          392 g          356 t
ORIGIN      
        1 ctcccgtatt tatgaatagt gaaggaaatg gcccgctccg gcgggcctac tgcatttcca
       61 gaatgaacaa acctatcgac caaagactca ttgacctcat ccggttgatc gttgggttgc
      121 ttgtatcagg cgactacgat gcaatcgaga gtcgttcccg tggtgtcagg ctcaatgccg
      181 agcaaatgaa agaagctgtg caaacacatg ggcacacgct tcaaagctta cctgagactt
      241 cttatgcgga attagatgtc atccgggtta ttcgtgcgac ctgccctacg tggtcaatta
      301 ctgttccgtt gtggaccatt gaagagggtc gttcagactt aacgctcgag tgcaccgtta
      361 tcgataacgg ccttccagaa ttggacatcg aaatcgataa ccttcacgta ctctgataat
      421 tccctctctg aaagaggccc gccaacgcgg gccatttttt atcgattgga gactgacatg
      481 accaccacat ctatggcgat caccgtctca tacagccaga ttgctgtttt ttcgagccac
      541 ttggagaacc ccttcaacaa ctggcaagaa caccacgtca cccaagggtt ttcgtggcgt
      601 gaacatagtg tttcgtttcg cacgctcaat gaatttggct cctgctcaat cgaaatagac
      661 ttctctgaat gccatccagg ttcggatgat gcaattcggg ctattagagt gccgttttcc
      721 gttccggagg atggcaagat tgagatcggc agcattggag attcggtgcc cctttcaatc
      781 gcgcccggcg cttatgcgtt gctcttccaa atcctcgcgg taagacctga cggaaaagaa
      841 gtaattcgcc tctctttctc taaaagtgag cccatggatt ttgcgattat taaagcagat
      901 gagcagatca caatcagcgg tccgctttgc aaaacgtcgc aggcggcaac ttgagccaaa
      961 cattggcccg cttcggcggg cttttttgca gatcaggagc acgcagatct gtaagctgat
     1021 ggcattcctc acgaggtttc atgatgactg cagccagcga cgccgaccta aatcatctct
     1081 tcgggatcaa gaagccattc ctggccccgt tgcatctcag tacccctctg catacccccc
     1141 gccggctcac tcccgacgag gccgcacaga tatgcgagcg ggactccgct atcaagccga
     1201 ccacacggga caactgggag ctttcgccgg tatcgatcaa cggccttctg cacgcggcgc
     1261 tgtcgaaaat gagcaaccgc ggcggccgcc atgctgaatc gacctggcac ctccccacgg
     1321 caggcgttgg ccaacttcgg ctgtcgtcgc ccctgggctc cggccgcgcg ctgaccaagg
     1381 acgaatgtcg gcagctccga tccatcgacg accggttcgc tggtgcgaac ccggcatgga
     1441 cggtgaagcc ctcgctctca aatccgctac aggtcgatat cgtccggtac cagaacgtga
     1501 acatgtggac ggttgggcaa ctttcgttgg cgcccgtac
//
LOCUS       DAOPTR010001009         1539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_63622_length_1539_cov_3.367925, whole genome shotgun sequence.
ACCESSION   DAOPTR010001009 DAOPTR010000000
VERSION     DAOPTR010001009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63622_length_1539_cov_3.367925"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="PKI05_15345"
     CDS             complement(<1..1247)
                     /locus_tag="PKI05_15345"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011124127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOA53628.1"
                     /translation="MSGQAGIVNVMKETRLFPPPPEFSAKARIKTMEQYREMWERAKN
                     DPEGFWGEYAQELFWFKPYEKVLEWNEPFATWFKNGQTNISYNCLDVHLNTWRRNKVA
                     YIWEGEPGDVRVLTYEMLHREVCKFANVLKSMGIQKGDRVSIYMPMIPELPIAMLACA
                     RIGAIHSVVFGGFAAEALAERNNDAQAKMVITADGGWRRGKIVPLKENVDIALEKSPS
                     VKQCIVVKRTGQEINMVQGRDFWWHDLMAKASANCPAEPMDSEAPLFLLYTSGSTGKP
                     KGVKHTTAGYNLYAKKTMEWVFDVRDEDVYWCTADIGWVTGHTYIVYGPLSAGVTTVM
                     YEGAPNWPAEDRWWDIIERYRVNILYTAPTAIRTFIKWGDEHVEKHDLSSLRLLGSVG
                     EGINPEAWIWYYEKIGGGRCPIV"
BASE COUNT          337 a          449 c          393 g          360 t
ORIGIN      
        1 tccacaattg ggcaacgacc accaccgatc ttttcgtagt accaaatcca ggcttccggg
       61 ttaattcctt cacccacact ccccagcagc cgcaggctcg agagatcgtg tttttcgaca
      121 tgttcatcgc cccacttgat gaacgtgcgg atggccgtgg gggccgtgta aaggatgttg
      181 acgcgatacc gttcgataat gtcccaccag cgatcctcgg caggccagtt cggagcacct
      241 tcatacatca ccgtggtgac acctgccgac agcgggccat acacgatata ggtatgccct
      301 gtgacccaac caatatccgc cgtgcaccaa tagacatctt catcgcgcac gtcgaatacc
      361 cattccatgg tcttcttggc atacaggtta taaccggccg tggtatgctt gacgcctttc
      421 ggtttgccgg ttgaaccact ggtgtaaagg aggaatagcg gggcttcact gtccattggt
      481 tcagcgggac aattggccga ggccttcgcc atcaaatcgt gccaccagaa gtcccgacct
      541 tgcaccatgt tgatttcctg accggtgcgc ttcacgacga tgcactgctt cacggacggg
      601 gacttctcca gtgcgatgtc cacgttctcc ttgagcggaa cgatcttacc acgccgccaa
      661 cccccgtcgg ctgtaatgac cattttcgcc tgggcatcgt tgttgcgttc ggccagggcc
      721 tcggcggcaa atcccccaaa cacgaccgaa tgaattgcgc cgattcgggc acaggcgagc
      781 atggcgatag gtaattcggg gatcatcggc atgtagatcg aaacgcgatc gcccttttgt
      841 attcccatgc tcttgaggac attggcaaat ttgcagacct cacggtggag catctcgtag
      901 gtcagcacgc ggacgtcacc cggctcgcct tcccagatat aagcgacctt attgcgtcgc
      961 caggtattca agtggacatc caggcaattg tatgagatgt tggtttggcc attcttgaac
     1021 cacgtcgcaa acggctcatt ccactccaag actttttcat agggcttaaa ccagaaaagc
     1081 tcctgagcgt attcgcccca aaatccctct ggatcattct tggccctttc ccacatttcc
     1141 ctgtattgtt ccatcgtctt gatccgcgcc ttagcggaga actcaggcgg cggcgggaac
     1201 aaccgggttt ccttcatgac gttgacaata ccggcttgtc cggacatcga gttaacctcc
     1261 ctttcctgtg agacctactc tgacaaattg acacgaatag ttgctgtcgg gaacttgtcg
     1321 caatcgacca atcgatcttg aaacggggat tgtagattct gaactaacga ctgtcaataa
     1381 tgccgcgcaa gccgaatcac aggacccacc gaggccgctc gaggttgccg cggcgatcca
     1441 ttgacggcgc cacgcttgac agacccgttt gcgttgccgt gttcgaccga agctgggatt
     1501 ggcggattgg ggattcggca aaggaaagag gccgaatta
//
LOCUS       DAOPTR010001010         1539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_63629_length_1539_cov_3.041105, whole genome shotgun sequence.
ACCESSION   DAOPTR010001010 DAOPTR010000000
VERSION     DAOPTR010001010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63629_length_1539_cov_3.041105"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PKI05_15350"
     CDS             complement(<1..513)
                     /locus_tag="PKI05_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53629.1"
                     /translation="MKPFPSRAAKVKLAISLAVCLSVVGFAFLYAQTRRGAKEGAKPI
                     VDQPTVEQAGGEDSAGKKVRTELVRRLLAKLDSLDDISAEDKKKLERILLEADAELAQ
                     AADRPRATGRGFRSGDRPTKGQPPQVGQEPSREQGAVTARGAQTPSTQASEGQGQPET
                     PTEAETPMVRA"
     gene            complement(636..1421)
                     /locus_tag="PKI05_15355"
     CDS             complement(636..1421)
                     /locus_tag="PKI05_15355"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOA53630.1"
                     /translation="MSHRTLNGIRGVVTGASSGIGQAFVKALLPFRPRLLVVARREER
                     LQQLSQLGAQYGAVVIPFACDVSQPEAAKRICEVAHQNFEGIDLLVNNAGIGALGRFE
                     DSPPEELRQVINVNLFSLVETTYYLLPLLKKGRRPIIVNVSSILGHRGIPWRSYYCAS
                     KFAVRGFSEAIRAELARHGIDVLVVSPGRTATELFEGPLEKGKTPAWPDPKPVSAEYV
                     AQRMLRAIQRGRHEIIPHPWGRLMVLVSRLAPRLMDWILQRYG"
BASE COUNT          329 a          444 c          422 g          344 t
ORIGIN      
        1 tgcccgaacc ataggggtct cggcctcggt gggagtttca ggctgcccct gaccctcgga
       61 tgcctgtgtg gacggtgtct gcgcaccacg ggctgttact gctccttgtt cccttgaggg
      121 ttcttggcca acctggggag gttgcccctt agtggggcga tcgcccgagc ggaagccacg
      181 gcccgttgct cgcggcctgt cagcagcttg ggcaagctct gcatcggctt caagcagaat
      241 cctttccaac ttcttcttat cctctgcgct aatgtcgtcc agagaatcga gctttgctag
      301 caatcgccgt accagctcgg tgcgcacctt tttacctgcg gaatcctcac caccagcttg
      361 ttcaaccgtt ggttgatcca cgatcggctt tgctccttct ttggcacctc gccgtgtttg
      421 ggcataaaga aaagcaaatc cgaccaccga cagacaaacg gctaggctga tggccaattt
      481 caccttcgct gcgcgactgg ggaatggctt catgggtggc cctacctttg tgcagggtaa
      541 tctccactag ggagttgaca aaccaccaaa attggactcg cggataacgc gctattaata
      601 aggtacgcaa aatgcgcaga ataagcaaga aaaaattagc cgtacctttg cagaatccaa
      661 tccatcaacc gcggggccaa gcggctgact aggaccatca gtctccccca cggatggggg
      721 attatctcgt gccgcccacg ctgaattgcc cggagcattc gctgggccac gtattcggcg
      781 ctgactggct tcgggtcggg ccacgcgggg gtcttgcctt tctccagggg tccctcaaaa
      841 agttcagtgg cagtcctccc tggactgacg acgaggacgt cgatcccatg ccgggccaac
      901 tccgctcgaa tggcttcgct aaatccccga actgcgaact tgctggcgca ataatagctc
      961 cgccacggga taccgcgatg tcctagaatg gaactcacat tgacaatgat cggacgtcgt
     1021 cctttcttaa gcaatggcag caagtaatag gtggtctcga ccaggctgaa gaggttcaca
     1081 tttatcacct ggcgaagctc ttctggaggc gaatcctcaa agcgaccaag ggcaccaatt
     1141 cccgcgttat taacgagaag atcaattccc tcaaagtttt ggtgagccac ttcgcagatt
     1201 cgtttggcgg cctccggctg gctcacgtcg caagcgaagg ggatcaccac ggccccgtac
     1261 tgtgctccta attgagaaag ctgctggagt cgctcttcgc gtcgtgccac aacgagcaag
     1321 cggggacgaa agggaagaag ggccttgaca aaggcctgac caattccgct ggacgccccg
     1381 gtaacaacgc cacgaattcc gtttaatgtt cggtgggaca tgtttgtagt gccacgaatc
     1441 tggaaacctt aacgttcgga gggggtggta ggctcaagct tgagtcattc gctgaccatg
     1501 ccggccgagt gaatttgata cgccgccatt tcccggttc
//
LOCUS       DAOPTR010001011         1537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_63738_length_1537_cov_3.254386, whole genome shotgun sequence.
ACCESSION   DAOPTR010001011 DAOPTR010000000
VERSION     DAOPTR010001011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63738_length_1537_cov_3.254386"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..>1537)
                     /locus_tag="PKI05_15360"
     CDS             complement(208..>1537)
                     /locus_tag="PKI05_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53631.1"
                     /translation="HVEIEAEEEVYRYTPANNGAGPMWCRGSTCLVRVGDDVFASGLE
                     TLPDVKPLNNCRWLLFHRTEQGWKQVQADLSGRTREPCPLVTFAGGPVFLSANPTLVE
                     DPNVPAGPARPEILQFDPSRPSEPPKVLLPRWMGEPNFTEHSYRSFAADGLRGELILF
                     QNVGYTHVAWAFLDQQGQWSAQGQLDFPFGAEYEKPQPIRVCYPTVAIKDRAVYFCGV
                     SDILEPNSAWREFKRKLTGRDWDYDFRRLFFTWSRDITRGVFEPWIEIASREKTAGWI
                     TPADLWVAPDGTVHILWLERAIDTRLREKFFPNEKQSEALNYAVIHDGEVVRRTSLIE
                     WHEGQPGLIPTAARFHITPEPRLLVFCHVIETGQAGKRFAGNLLFEVLNDGSAGPVMQ
                     VPLRYPLDSYFVATPRAGSPPSYTLDLLGTTRGDPLTIRYARLKVSPKAP"
BASE COUNT          336 a          466 c          442 g          293 t
ORIGIN      
        1 cctaaccctc tccccgaggg agagggggac tttgggatag gctctaaatg caaaatctgc
       61 ctctgacaac caaatgccct cccaccaact gccggttacg ctcgaagcgc tgccatcacg
      121 tgctgccgct taaggacggc atggaccgtc gggggctacc gaaccgatac cccgccgctt
      181 tctcttgaaa gcttccccgg ccggcgccta gggggccttg ggagagacct tgagccgtgc
      241 gtagcggatg gtcaaagggt caccgcgcgt cgttcctagc aagtccaacg tgtatgaagg
      301 aggcgaaccc gcccgcggcg tggccacgaa atacgagtct agaggatacc tcaacggcac
      361 ctgcatcacc ggaccggccg aaccgtcgtt taagacctcg aagagcaaat tcccggcaaa
      421 ccgcttgccc gcttgcccgg tttctatgac atggcaaaaa accaacagcc gcggttctgg
      481 ggtgatgtga aatcgggcgg ccgtgggtat cagcccgggc tggccttcgt gccactcgat
      541 caagctcgtg cgacgcacga cctcaccatc atggataacg gcgtaattga gggcttccga
      601 ctgtttttcg ttgggaaaga acttctcgcg cagtcgcgta tcgatggcgc gttctaacca
      661 caggatgtgc accgtcccat caggcgcgac ccacagatcc gccggcgtga tccaaccggc
      721 cgtcttctcc cgtgacgcga tttcgatcca cggttcgaat accccgcggg taatatcgcg
      781 gctccatgtg aagaaaaggc ggcgaaaatc gtagtcccag tccctgccgg tgagttttcg
      841 cttgaattct cgccacgccg agttaggctc cagaatatcg ctgacgccgc aaaaatagac
      901 ggcccgatct ttgatcgcta ctgttggata acagacccgg atcggttgag gcttttcata
      961 ctccgcaccg aagggaaagt ccaattggcc ctgcgcggac cactgaccct gttggtcgag
     1021 aaaggcccac gccacgtggg tatagccgac gttttggaaa aggatcagct ccccacgaag
     1081 accatcagcg gcgaagctgc ggtaagagtg ctcggtgaag tttggctcac ccatccaacg
     1141 cggtaagagg accttagggg gctcactggg acggctgggg tcgaactgaa ggatttccgg
     1201 tcgggcggga cctgccggga cgttagggtc ctccacgagc gtgggattgg ccgagagaaa
     1261 caccggacca ccggcgaagg tcaccaaggg gcaaggttca cgcgtccgac cactaaggtc
     1321 agcctgcacc tgtttccagc cctgttcagt gcgatggaac aagagccagc ggcagttatt
     1381 caaaggcttt acgtcgggca aagtttccag cccactggcg aatacatcgt ctccgacccg
     1441 caccaggcag gttgaacctc tacaccacat gggaccggca ccattgttag ccggagtgta
     1501 acgataaacc tcctcctcgg cttcgatctc aacgtgc
//
LOCUS       DAOPTR010001012         1534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_63915_length_1534_cov_3.329277, whole genome shotgun sequence.
ACCESSION   DAOPTR010001012 DAOPTR010000000
VERSION     DAOPTR010001012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63915_length_1534_cov_3.329277"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="PKI05_15365"
     CDS             <1..854
                     /locus_tag="PKI05_15365"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOA53632.1"
                     /translation="YRRLGKTELMVSAVCLGGHSRSDQQLRNEIISRCIDLGINYVDA
                     CWDNEVIRDAKALKGRRDKMYLALSHGAKEVRNEEYRTAKKLLESLDELLKASEQEYT
                     DLWRITCLEPGGRHSFDTSCEIVDALTKAKQQGKARFVGISSHDRRWLKFMIEYFPQI
                     DVILFPFTTMSKAAPKDSLFPAIKQCDVGAFGIKPFGAGSLFSGNKAEDMRRARLAIR
                     YILATNTVVPIPGANSLEEVDNLALAVKERRSLDVKEQAELDQLNRHMWANLPRGYQW
                     LKNWEYV"
     gene            906..>1534
                     /locus_tag="PKI05_15370"
     CDS             906..>1534
                     /locus_tag="PKI05_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53633.1"
                     /translation="MGVLRGLGWVLLLLVVGCSRAATENRSDAGQQAGPTSLSQAEAV
                     PVAPSAEQPSPSSQADEEENMTATATEESSAGNNSTEEPLLLLEESDDGQDTHLMADN
                     SRCFVCHLNFQFEDIALVHAREGYGCAYCHGPSDAHIADESWASGGNGTAPDIMYRPN
                     EVIPACLKCHELSKSDPECHCDFPRLPQKKSCTDCHGNHRLKERTCHWK"
BASE COUNT          358 a          404 c          434 g          338 t
ORIGIN      
        1 agtatcgtcg tctgggaaaa acggagttga tggtctcggc tgtctgtttg ggtgggcatt
       61 cccggagcga ccaacaattg cgaaacgaga ttattagtcg ctgcattgac ctgggaatta
      121 actatgttga tgcgtgctgg gataacgagg taatccgcga tgccaaggct ttgaaaggcc
      181 gccgcgacaa gatgtatctt gccctctccc atggggccaa agaagtccgc aatgaggaat
      241 atcgtacggc caagaaactt ctcgagtcac tcgatgagct cctcaaggcg tcggaacaag
      301 aatacaccga cctttggcgg attacgtgct tggaaccagg aggacgtcat agcttcgaca
      361 cctcctgcga gattgtggac gccctgacca aggccaagca gcagggaaaa gcccggttcg
      421 tgggaatttc ctcgcacgac cgccgatggc tgaaattcat gattgaatat ttcccgcaga
      481 ttgatgtgat cctcttcccc ttcaccacca tgagcaaggc ggccccgaag gatagcctct
      541 tccccgccat caaacaatgc gacgtcgggg cctttggaat taagccgttc ggcgccgggt
      601 ccctcttttc gggcaataag gcagaggaca tgcgacgggc acgtttggcc atccggtata
      661 tcctggccac caataccgtg gttcctattc ccggtgcgaa tagcttggaa gaggtggaca
      721 atcttgccct cgccgtcaag gaacgccgta gcttggacgt aaaagagcaa gccgagcttg
      781 accagttgaa tcgtcacatg tgggcgaact tgcctcgcgg ctaccaatgg ctgaaaaact
      841 gggagtatgt ctgatccggt gtggtgaagt attctgggtg ccgttccgct ttggggtatc
      901 tacagatggg tgttttgcgc ggtcttggat gggtgctcct gttacttgtg gtcggttgtt
      961 cccgagcggc cacggaaaat cgttcggatg caggccaaca ggcaggtccg acgtcgctta
     1021 gccaagcgga ggccgttccg gtagcgcctt cggccgagca acccagcccg tccagccaag
     1081 ccgatgaaga agagaatatg accgccacgg ccacagaaga gtcttccgcc ggtaacaatt
     1141 caacggaaga acccttgctt ctgttggaag aatcagatga tggacaggat acccatttga
     1201 tggccgataa cagccgttgt ttcgtctgtc atcttaactt ccaatttgag gacattgcat
     1261 tggtccatgc cagagaaggc tatggttgtg catactgtca tggcccgagc gatgcccata
     1321 tcgccgacga gtcttgggcc tcaggtggaa acgggacggc acccgacatc atgtaccgcc
     1381 caaacgaggt gatcccggcg tgcctcaagt gtcacgagct ttccaagtca gacccggaat
     1441 gccactgcga ttttcccaga ttgcctcaaa agaaatcgtg caccgattgc catggcaatc
     1501 atcggctaaa ggaacggacg tgtcactgga aata
//
LOCUS       DAOPTR010001013         1527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_64342_length_1527_cov_3.486413, whole genome shotgun sequence.
ACCESSION   DAOPTR010001013 DAOPTR010000000
VERSION     DAOPTR010001013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64342_length_1527_cov_3.486413"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="PKI05_15375"
     CDS             complement(<1..1074)
                     /locus_tag="PKI05_15375"
                     /inference="COORDINATES: protein motif:HMM:NF036484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HOA53634.1"
                     /translation="MAASDIVVQGAREHNLRNVNIVLPRNHLICLTGVSGSGKSSLAF
                     DTLYAEGQRRYIESLSTFARQFIGQMPKPNVDRIEGLSPSICISQKTAGSNPRSTVGT
                     ITEIYDFLRILYARAGTAYCPQCGERIQAQPRQLIVEQILESFTGKTVLVLAPLIRGQ
                     KGEYQSLLATLRRRGFARARIDGEVVRLDQDLRLRRHMRHDIELVVDRLEVSPAQRSR
                     LAEAVESAIGLSDGEVMVVCEGNEATDGSQQRGTARGLVRRFSSRFACPNCQISFAQP
                     TPQLFSFNSPEGMCQRCQGLGQVETVDPRKFIENPHRSIQQGVISALGRWKEMLPDVR
                     YLLRRWASILEEELGYEKGALLET"
BASE COUNT          379 a          450 c          385 g          313 t
ORIGIN      
        1 cgtttccaag agtgcccctt tttcataacc taactcctcc tcaaggattg acgcccaccg
       61 ccgcaaaagg tagcggacgt ccggcaacat ctccttccac cttcccaacg cagaaatgac
      121 gccctgctga atggagcgat gggggttctc aatgaatttc cgtggatcga ccgtctcaac
      181 ctgtcccaac ccttggcacc gctgacacat tccttcggga ctattaaagc taaacaattg
      241 tggcgtcggt tgggcaaagc tgatttgaca gttgggacag gcaaatcgtg aagaaaaccg
      301 ccggaccagg cccctcgcgg ttccgcgttg ctgcgaaccg tctgtcgcct cattcccctc
      361 gcaaacgacc atcacctcgc cgtcgctcag acctatcgcc gactcaacgg cctcggccaa
      421 tcgactccgc tgcgcggggc taacctccag ccgatcaacg accagctcga tgtcgtgccg
      481 catgtgtcgt cgcagacgga ggtcttgatc gagccggacg acttctccat caattcgggc
      541 acgggcaaaa ccgcgccgac gcaacgtggc cagcaggctt tgatactcac ccttctgacc
      601 gcggatcaac ggggcgagga ccaaaaccgt ctttcctgta aacgactcca gaatctgctc
      661 gacgatcagt tgccgcggct gggcttgaat ccgctctcca cattgcggac agtatgccgt
      721 ccccgcccgc gcatagagaa tcctcagaaa atcgtaaatt tccgtaattg tgcccacggt
      781 tgagcgaggg ttagacccgg ccgttttctg tgatatacag atggaagggc tgaggccctc
      841 aatccgatcg acgttgggtt tgggcatctg gccgatgaat tggcgggcga aggtggagag
      901 gctttcgatg taacgccgct gtccttcggc gtaaagggta tcgaaagcca gagaactttt
      961 cccagagccg ctgactcctg tcaggcagat cagatgattg cggggtaaga caatattgac
     1021 attgcgaagg ttgtgctccc gtgccccttg cacaacgata tcggaggcag ccatgcgaga
     1081 ttacccgaaa aaaagcacta gcggccgatc agaatcttaa cgctcagaac gaaccgggcg
     1141 agaacctacc attataccaa acccgcatta tagggctttt tcgaaaccag cgacaagtta
     1201 cagattctca ccacagattc tcaagattac gcagtcgaaa agcaaattac ccagttcggc
     1261 gttttttacc cgcgagaatg gaccaaccgc cgaacaggac ctcgaggaaa aaactattcc
     1321 atttttgtgg cgcgaaatac actgtggtaa cgatgtcccc cattcagaga aaatagacaa
     1381 cttgcgatga ctggaaaaat ttttgataaa caaaacaggt ctcgggagct ttagcctgcc
     1441 gtgccgcaaa atggcctgac atgtcccggc gccggtccgc tccaggaggt cttcgggcac
     1501 gaaaacaacc acgcccaaac cccgaaa
//
LOCUS       DAOPTR010001014         1526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_64395_length_1526_cov_4.049626, whole genome shotgun sequence.
ACCESSION   DAOPTR010001014 DAOPTR010000000
VERSION     DAOPTR010001014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64395_length_1526_cov_4.049626"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1438)
                     /locus_tag="PKI05_15380"
     CDS             complement(<1..1438)
                     /locus_tag="PKI05_15380"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA53635.1"
                     /translation="MNQNQPNVLVLNIEGLRAEFLGPYGNDWIDTPHFNRLAVEGFVF
                     DHCLVGGVNSCDQLTALWSGCHPSQALPTRFAIDAGKAQWLVEVFRRAGYFTILTSSS
                     ESVTKHPGASAFDEHRLVAPVKEAAPATSNEHLPVITDPEQLAPVGEPAADWSETRTA
                     AVFSELIQIIETQESPQFIWCQLTTLSAIWESPLELRERFREPGDPPVWPGTNIPRAF
                     FGPGTDPDVYLPFVQAYAAEVYILDLCLGGLLEVLKRNGDKRPWVVVLLSSRGLPLGE
                     HGHVGFAATCFYNELLHVPLIFSFPNGAGQAERSQALVYPHDIMPTLATACGLVDKSD
                     TSLESLPPAGTCETVWIAGRNLLPVIEGSCNFVRNRLFSYSGADVLAIRTPAWLAQIL
                     LSEDGDGPGDLHEQVPERQIPFVDHLKAIVDADDDSEAQNAEDAPQEELLSRVEIAEL
                     RKHVRLFVKPDDRWEINEVADRCPDVAVE"
BASE COUNT          359 a          437 c          399 g          331 t
ORIGIN      
        1 actcgacggc cacgtccgga caacgatcgg caacctcgtt aatctcccaa cggtcatcgg
       61 gtttaacgaa aagccggaca tgttttcgca gttcagcgat ctctacacgg gatagcaatt
      121 cctcctgggg ggcatcttcc gcgttttgtg cctcggaatc atcatcagcg tcaacaattg
      181 ccttaaggtg atcgacgaag ggaatttgtc gttcagggac ttgctcgtgc aaatcgcctg
      241 gaccgtcgcc gtcttcagac aagagaatct gcgctagcca tgccggcgtg cgaatggcaa
      301 gcacatcggc gccactataa gaaaaaagac gattacgaac gaagttgcag cttccctcga
      361 tcaccggcag caaattgcgc cctgcgatcc acaccgtttc acaggtgccg gcgggcggga
      421 gcgactcaag cgatgtgtct gacttgtcca ctaatccgca tgccgtggcc aaagtcggca
      481 tgatgtcgtg aggataaacc agcgcctgtg atctctctgc ttgtcccgcg ccgttcggaa
      541 aactgaagat cagcggcaca tggaggagct cattgtaaaa acatgtcgct gcaaaaccta
      601 cgtgcccatg ctctcctaac ggcaatccgc gagacgaaag taacacaacg acccacggtc
      661 ttttgtcgcc gttgcgtttc aggacttcga gaagtccacc caagcaaagg tcgaggatgt
      721 acacttcagc cgcataagct tgcacgaacg gcaaatacac gtcgggatcg gtacctggtc
      781 cgaagaatgc tcgtgggata tttgttcccg gccacacagg tgggtcaccc ggttcgcgaa
      841 atctttcgcg cagttccagc ggcgattccc aaatcgcact caatgtcgtc agttggcacc
      901 aaatgaattg cggagattcc tgggtttcaa tgatctggat aagttccgaa aagacggcag
      961 ccgttcgtgt ttccgaccag tctgcggccg gttcgccaac aggagctagt tgctccggat
     1021 cggtgatgac tgggagatgc tcatttgacg tcgcgggagc cgcctctttc acaggggcca
     1081 ccaaccggtg ttcatcaaaa gcactggcac caggatgctt ggtcaccgat tcactcgatg
     1141 aagtcagaat cgtgaaatac ccggctcgcc taaatacctc aactaaccac tgcgctttcc
     1201 ccgcgtcgat cgcaaacctc gtaggaaggg cctggctcgg gtgacatcca ctccatagtg
     1261 cggtcagttg atcacatgag ttgaccccac caaccaagca gtggtcgaag acaaagcctt
     1321 cgaccgccaa acggttgaaa tggggcgtgt cgatccagtc atttccgtaa ggtccgagga
     1381 actccgcccg aagcccttca atgttcaaaa caagaacatt tggctgattt tgattcatgg
     1441 ccgagaggct ctccctagcg acgaggttta acctgctctt cccatcgggc aagcatcttc
     1501 cgtagcactg ccgctcggtc cggttg
//
LOCUS       DAOPTR010001015         1526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_64422_length_1526_cov_2.445275, whole genome shotgun sequence.
ACCESSION   DAOPTR010001015 DAOPTR010000000
VERSION     DAOPTR010001015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64422_length_1526_cov_2.445275"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="PKI05_15385"
     CDS             complement(<1..764)
                     /locus_tag="PKI05_15385"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOA53636.1"
                     /translation="MRILILGAGTIGASIAEFLCQTHRVSLIDRDQEAVDRVNETMDV
                     RALCGSAADAVLLFQAGATDADLCLAVTNSDEVNLVGASVAKAMGTRRAIARVYSPNL
                     LDTSTFDYRRHFKLDYLVSLERLAALDLARVVRSPGALSIETFAVADLEMQEIVVQPG
                     APAIDIPLRELRVGRHVRVGVISRGDKVFIAGPEDAVAVNDRIALVGKREDIDAVKSL
                     FQTEPPRRQGVVIAGGGETGFHLARLLEGPRFNVVL"
     gene            complement(992..1399)
                     /locus_tag="PKI05_15390"
     CDS             complement(992..1399)
                     /locus_tag="PKI05_15390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOA53637.1"
                     /translation="MAAFLRDVHERAVALGCAEINILTVNGEPVAFAYNYVNSGRVFG
                     LRTAYDPAYAGASPGAYLRFCMIRDSFDRGDREVNLGPGYLESKKHWATELRYSYRYT
                     YFPGSPRAQLLRLKRLWDNRFRSAEHLAKMRAI"
BASE COUNT          354 a          464 c          418 g          290 t
ORIGIN      
        1 atcaggacca cgttgaatcg cggtccttcc aagaggcgtg ccaggtgaaa gccggtttca
       61 cctccccccg caatgaccac accttgccgc cgcggtggtt ccgtctggaa aagcgatttc
      121 acggcgtcaa tgtcctcacg ttttcccacc aaggcaatgc gatcattgac ggcgacagca
      181 tcctccgggc cagcgatgaa aaccttgtct ccgcgggaaa tcacgcccac gcggacatgc
      241 cgacccacac gaagctcccg caacgggatg tcaatggcag gtgcgcccgg ctgaaccaca
      301 atttcctgca tttccaggtc ggctacggca aatgtctcaa tactgagggc gcccggacta
      361 cgaacaactc gggcgaggtc taaagcggca aggcgctcca agctaaccag gtagtccaat
      421 ttgaaatgac gacgatagtc gaacgtgctt gtgtcgagaa gattgggcga gtaaacgcgg
      481 gcaatggccc gacgggtccc catcgccttg gccacgctcg cccccacgag attgacctcg
      541 tccgaattgg tcaccgccaa acaaagatcc gcgtccgtcg cccccgcttg aaagagcaaa
      601 acagcatcag ccgcggaccc gcatagggcc cgcacgtcca tggtctcgtt cacgcgatcc
      661 acggcttcct gatcgcggtc aataagcgat acccggtggg tctggcacaa aaactcggcg
      721 atcgaggcgc caattgtccc agcaccaaga atcaaaatcc tcatgcgtca tgttcccgaa
      781 acggacgatc aactacctgt tatcccacgg ataggttggg gggattcgac caggtgccca
      841 gcgacagggc caaaacggcc attatatccg aaatggcctt ggttgagggc acctaacagt
      901 taatatgaga agactagagg ctatgggaaa aatgatgctc gggggtacct ggggcagcat
      961 cagtgatttg ggtgtattct gcgcggattg cctatattgc ccgcattttt gctaagtgct
     1021 cggccgaacg aaaccgattg tcccacaatc gtttcagtcg gagaagctga gctcgaggcg
     1081 aacccgggaa gtaggtatac cgatagctat accttaactc ggtggcccaa tgttttttgg
     1141 attccaggta gccgggaccg aggttcacct ctcgatcgcc ccggtcgaag gaatctcgaa
     1201 tcatgcaaaa ccgcaaatat gccccgggac tggcgccggc ataggccgga tcgtaggcgg
     1261 tcctcaatcc aaatacccgc ccgctgttga cgtaattgta cgcgaaggcg accggctcgc
     1321 cgttgaccgt gaggatattg atctccgcac atcccaacgc aacggcccgt tcgtgaacat
     1381 cgcgaagaaa tgctgccact tcgcctgccc caatccccag aaaccgcgcg cgatttcacc
     1441 gagatgcagg cccgcattgt taatgagcac gtcgagtttg gggtgcgccg ccgtcaccgc
     1501 cctgctcggc cccaacggtg cgggca
//
LOCUS       DAOPTR010001016         1522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_64635_length_1522_cov_5.132924, whole genome shotgun sequence.
ACCESSION   DAOPTR010001016 DAOPTR010000000
VERSION     DAOPTR010001016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64635_length_1522_cov_5.132924"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(312..1238)
                     /locus_tag="PKI05_15395"
     CDS             complement(312..1238)
                     /locus_tag="PKI05_15395"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XcyI family restriction endonuclease"
                     /protein_id="HOA53638.1"
                     /translation="MIEHPEDRPWFVDQLTKSAFFHQKLHEWKMLPIAEAIEAIRGED
                     LSWEKQELGITESAWNKVIHRGIKPVTVFAHPQVLIQIRGATSYYRMLSMVSQKSMGR
                     VGLPVTSYETGSCVPEEPAAKQIACHLNLIISRLIDTDVQINPREFDLWRGMAAGTQA
                     QGSWQNAKGQHAELLIKGLIKRRLFDAGWVSRETEEDQRLELSDGRIVSFGSEPDVAF
                     YKRQNIQAAVEVKGGIDSAGVLERLGAAMKSLSRTREKNPTAVTILVFFKASLTEKAK
                     QDLELNRKIITHWFTVEELLNYEFDRQGLSGA"
     gene            complement(1235..>1522)
                     /locus_tag="PKI05_15400"
     CDS             complement(1235..>1522)
                     /locus_tag="PKI05_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53639.1"
                     /translation="IPDMLTAYFVDMQKTISQWWKVLASSAHVVMVVDNVRFEGQMLP
                     VDLILSEMAEQEGFAVEEILVARYKGNSSQQMGRYGRLPVRESIVVWRKQE"
BASE COUNT          358 a          426 c          352 g          386 t
ORIGIN      
        1 cctattcaat tgcatgggga attctgggtt ggaatacggt attctgggaa gtgaggaaat
       61 attgatatgg actatctgaa gatatatcaa agcttgggag tttcgataga agctcccaaa
      121 ggtagcaccg gatgacggaa agtaatgccc caggacggcc aagtggccca ctcaaccccc
      181 aggctttgcg gctggaagac atggcccggc tcttgtcggc cgctggcccg aaggcggtca
      241 ccatggaaat gctccaagcg gacatcgctg cgggggcccc ccctgccccc cgcaaaccca
      301 attgcgcaat tttaagcacc cgataatcct tgtctatcga attcgtaatt caataactct
      361 tctaccgtaa accaatgagt aatgatcttg cgattcagct ccaaatcttg ttttgccttt
      421 tcggtcagag acgctttgaa aaaaaccagg atagttaccg ccgtagggtt tttctctcga
      481 gtgcgactga gacttttcat ggcggccccc aaccgctcca gtacccccgc agagtcaatc
      541 ccgcccttaa cctccactgc cgcttgaata ttctgcctct tataaaaggc cacatcaggt
      601 tccgagccga acgaaacaat tcgaccatcc gacagttcca atcgctggtc ttcctccgtt
      661 tcccgactaa cccatccggc atcgaaaagc ctccgtttaa taaggccctt gatgagcagt
      721 tccgcatgtt gacctttagc gttttgccac gatccttggg cttgagtccc ggcggccatt
      781 ccgcgccaga gatcaaattc tctgggattt atttgtacat ctgtgtcaat gaggcgactg
      841 atgatcagat tgagatgaca ggcaatttgt ttggccgccg gttcttccgg cacacaagag
      901 cccgtctcat aggaagttac cgggagtccg actcttccca tcgacttttg cgataccatc
      961 gaaagcattc gatagtagct agtggcccct cgaatctgga tcaacacctg ggggtgtgca
     1021 aacacggtaa ccggcttaat tccccgatga atcaccttgt tccacgccga ttcggtaatg
     1081 cctagttcct gtttctccca ggaaagatct tcgccgcgga tagcctcgat tgcttcggca
     1141 atcggaagca ttttccattc atggagtttc tgatggaaga atgcactttt ggtaagttga
     1201 tcgacgaacc agggtcgatc ctctggatgt tcgatcattc ctgttttctc caaaccacga
     1261 tgctttctcg tactggaagt cggccatagc ggcccatttg ttgggagctg ttgcctttgt
     1321 acctggcaac caggatttct tccaccgcaa atccctcttg ctcagccatc tccgaaagga
     1381 tcaagtccac cggcaacatc tggccttcaa accggacatt atcgacgacc atcaccacat
     1441 gagcggacga agccagcact ttccaccatt gagagatggt cttttgcata tccacaaaat
     1501 acgccgttag catatcgggg at
//
LOCUS       DAOPTR010001017         1522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_64641_length_1522_cov_3.615542, whole genome shotgun sequence.
ACCESSION   DAOPTR010001017 DAOPTR010000000
VERSION     DAOPTR010001017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64641_length_1522_cov_3.615542"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="PKI05_15405"
     CDS             <1..755
                     /locus_tag="PKI05_15405"
                     /inference="COORDINATES: protein motif:HMM:NF012677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53640.1"
                     /translation="QILAERNRRPLIMYDAAHVLGILGPVFQDPLAEGADIVTGSTHK
                     TFFGTQRGIIASALNSDSPFAELWDHIRRRAFPGFLSNHHLGTLLGLLLAVMEMNVFR
                     QEYQSQVIKNAKAFARALRECNLQVEGDPAMDFTETHQVLLRVGSHRGIEAAQLLEEN
                     NIIVNYQALPGDENFAASSGLRMGVAEMTRFGMKEADFQELASFIADILLHKKQLGEK
                     VSQFRGRFLKMHYCFDPADWPDLTQKLLGTFS"
     gene            complement(846..1376)
                     /locus_tag="PKI05_15410"
     CDS             complement(846..1376)
                     /locus_tag="PKI05_15410"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOA53641.1"
                     /translation="MDVFEAINKRYSYRGPFRDVPVPREHLRKIVEAGIRAPSGKNAQ
                     TTSFVIVDDPVLIRQIAALFENKPVCSTAKAMIVCVTDPRPVMGDVSFHVEDCAAAVE
                     NILLAITALGYATVWIDGALRYNRIAERIGELLGIPSDRIVRVLLPVGVPAEPGTQRE
                     KLPFEQRAWFNGWKKS"
BASE COUNT          332 a          431 c          411 g          348 t
ORIGIN      
        1 ggcaaattct ggccgagcga aatcggcggc ctctcattat gtacgatgcg gcgcatgtct
       61 tgggtattct tggccccgtg tttcaagacc ctctggccga aggggccgac attgtgacag
      121 gttccaccca taagacgttc ttcgggacgc agcgaggaat catcgcgtct gctctgaact
      181 ctgattcacc atttgccgag ctttgggacc acattcgccg ccgtgcgttt ccgggatttc
      241 tcagtaatca ccacctcgga acgctcctcg gcttgctttt ggccgtcatg gaaatgaatg
      301 tctttcgcca agagtatcaa tcgcaggtga tcaagaacgc caaggccttt gcgcgggcac
      361 tgcgggaatg caatctccaa gtggaaggcg atcccgccat ggactttacc gaaacgcatc
      421 aggtgctgct gcgggtgggg tcacaccgcg gcatcgaagc ggcccaactg ctggaggaga
      481 ataatatcat cgtgaattac caggccctcc cgggtgatga aaattttgcc gccagttctg
      541 gcctgcggat gggcgttgcc gagatgaccc gctttggcat gaaggaggcc gacttccaag
      601 agcttgccag cttcatcgcc gacattttgc ttcacaaaaa acaactcggc gagaaggtga
      661 gccaattccg tggccgcttc ctgaagatgc actattgctt tgacccggca gattggccag
      721 acttgaccca aaagctactg ggcacgtttt cgtgaaaact tcggaatcgc gcaatacttc
      781 agcgtaatgg tggccgccat ggggctgccg tctgatcgtg ggcgcgattg tatctccagg
      841 accacttacg actttttcca gccgttgaac caggcccgtt gttcgaaagg taacttttcg
      901 cgttgagtgc caggttctgc tgggaccccc accggcaata agacgcgaac aatccggtcg
      961 gatggaatcc caagcaactc gccaatccgt tcggcaattc gattgtaacg gagcgcccca
     1021 tcgatccaca ccgtggcata gcccaaggct gtgatggcaa gcaggatgtt ttccacggcg
     1081 gcggcacagt cttccacgtg gaaggagacg tcgcccatca ccggtcgcgg gtccgtgaca
     1141 cacacgatca tcgccttggc cgtgctgcaa accggcttat tctcgaaaag tgcggcaatc
     1201 tgtctaatca atacgggatc atccacaatc acgaaacttg tcgtttgggc atttttgcca
     1261 gacggtgccc gaatgccggc ctccacaatc ttccgcaaat gttcgcgcgg gacgggcaca
     1321 tcccgaaatg gtccccgata gctgtaccgc ttgttgatcg cttcaaaaac atccatggcg
     1381 tctcctcccg aaatcaatgc atcgtgtggg ttattggcat accagctcgg cacgaggtgc
     1441 gttcttgtca acaaccgcga atgtctctat gttaccctta cactgccgtg gggttaagtc
     1501 tttcgtaggg caaaaggaaa gt
//
LOCUS       DAOPTR010001018         1522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_64655_length_1522_cov_2.859577, whole genome shotgun sequence.
ACCESSION   DAOPTR010001018 DAOPTR010000000
VERSION     DAOPTR010001018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64655_length_1522_cov_2.859577"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            347..1243
                     /locus_tag="PKI05_15415"
     CDS             347..1243
                     /locus_tag="PKI05_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOA53642.1"
                     /translation="MTNVQKYLKPEVARQIKRLDLRAQFIVKGFLHGLHASPFQGFSV
                     EFSEHRRYVPGDDPMDIDWLVYAKTDRYYVKKFEAETNITGYLVVDASRSMGYTYRQE
                     TTKFDYAICLAAALCYLMIHQQDPVGLWVFDEAIRHSLAPKSRRGHLGTVLSVLAKLE
                     PVGNTDIARCLAQIASILRRASLIILFSDLLTDPEPVLQGLHRLKHRGHDIIVFHILD
                     EAEVSFPFQGTVELEEPESGERLEVDAAAYRADYLEAVQQFREYYRKECFRAGIDYVP
                     LDTSMAFDKALMEYLLRRRARF"
     gene            1281..>1522
                     /locus_tag="PKI05_15420"
     CDS             1281..>1522
                     /locus_tag="PKI05_15420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HOA53643.1"
                     /translation="MGFVTPMLFWAGLGLATIPVVLHLIMRQKARRVVFPALRLVKQR
                     YEVRRRRLQLQHVLLMLLRMLLILLVGVGLARPKLE"
BASE COUNT          320 a          401 c          428 g          373 t
ORIGIN      
        1 tcgtctggtc gctgaggtgc ccgccccagc cattcccaag tttgaacagc cggctccgcc
       61 gcaactcaac ccggatatct tgcggccttt gacttagagt ccatcgccaa aagtctccct
      121 ctccctaggg gagagggttg ggatgagggc aagcgaacgt cgcagttgat tgagaaaacc
      181 ggcagcaatt cgagccaccc tcacccccga cccctctccc gcccaatggg agaggggagc
      241 ttatgggata ggctctttga gggcgacggg ctggtcgctg aaacggtggg atagcattag
      301 gggctaattc ccgcctggat aagatcttgg ggcttgtggc gaaattatga cgaacgttca
      361 gaaatatctt aaaccagagg ttgcacgcca gatcaaacgg ctcgacttgc gggcccagtt
      421 catagttaag ggatttttgc acggtcttca cgccagcccg tttcaggggt tctctgttga
      481 gtttagcgag catcgacgtt atgtccccgg tgacgatccg atggacatcg attggttggt
      541 ctacgccaag accgaccgct actatgtgaa gaaattcgag gccgagacca acatcacggg
      601 ttatctggtc gtcgatgcca gccgttcgat gggttacacc taccgccagg aaacgaccaa
      661 attcgattat gcgatttgcc tggccgcagc tttgtgctat ctcatgattc accagcagga
      721 cccggttggc ctgtgggtct ttgacgaggc cattagacat tcccttgccc ccaagtcgcg
      781 tcgcggacat ttgggaacag ttctttccgt attggcaaaa ctggaacccg tgggaaacac
      841 agatattgct cgatgccttg cgcagattgc ctcaattttg cgtcgggcaa gtctgatcat
      901 ccttttttcc gatcttttaa ccgaccctga gcccgtcctt cagggtttgc accgactaaa
      961 acacagaggg catgacatca ttgtgtttca tattctggac gaggctgagg tctcctttcc
     1021 ctttcaaggg acggtcgaac tcgaggagcc cgaaagtggc gaacgcctgg aagtggatgc
     1081 ggcagcctat cgggcagact atctggaagc ggtgcaacag tttcgtgagt actaccggaa
     1141 ggagtgtttt cgtgccggca tcgattatgt gccgctcgat acgagtatgg ccttcgacaa
     1201 ggccctcatg gagtatcttc tgcgacggcg agcaagattc tgacctttga catggcatgc
     1261 acgaataaag cgtttgtgag atgggatttg taacaccgat gcttttttgg gcaggattag
     1321 ggctagcgac gattcccgtc gtgctccatc tcattatgcg ccaaaaggcc cgtcgcgtgg
     1381 tttttcctgc ccttcggctg gtcaaacagc ggtacgaagt gcgacgacgc cggttgcagc
     1441 tccagcacgt cctcctcatg ttgttgcgga tgctgcttat tcttctggtt ggtgttggac
     1501 tggcgcggcc aaaactggaa tg
//
LOCUS       DAOPTR010001019         1522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_64659_length_1522_cov_2.693933, whole genome shotgun sequence.
ACCESSION   DAOPTR010001019 DAOPTR010000000
VERSION     DAOPTR010001019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64659_length_1522_cov_2.693933"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1522
                     /locus_tag="PKI05_15425"
     CDS             <1..>1522
                     /locus_tag="PKI05_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53644.1"
                     /translation="IMAARLSICLAVTVSLAFSAVSFADENDPYYVYITTAPEFQPVK
                     APANLALARWDTWIYMPWRYQWTIGTGDEGGQFCRDFGINGGFTDHGDGPFAWLEQWN
                     LRFYNDHTAGKGFLYLRDANQRSRFQAFQRDPRAIRQDREGLKPLDEALFGKLAELVR
                     TRVGRVRKSPMRVAYALDDEISWGIFVVPLPWRVHDDDAAYQAWLDRYYGGKGPKAQF
                     VTPDFILPQLKGRLADLDFSPLLDRITYNDSVWANFIGRLVEVANEVDPETPCGFVGG
                     QSPNIWGGYDYAKLMKKIQFIEAYDLGSSQAIIRSFNPGNAFPQVTTHFHSDARGTAN
                     DVWQSWYYFAHGNRGMIGWVEGWFDGTKPRPWLYEYRATLKELGETQGPKLFGARWVH
                     DGIAIYYSHPSIQVSWCLDMEAHGKTWVNRGNDHRLGTSHLVRKAWEYLLVDSGLQYS
                     FISYDEVVRNGIPPEYRVLILPACYALSDIEAERIKEFCKRGGTVIADFCCGLFDQH"
BASE COUNT          337 a          418 c          423 g          344 t
ORIGIN      
        1 atcatggcag cacgcttaag catctgcctt gcggtaaccg tttctctggc gttttcggca
       61 gtttcatttg ctgacgagaa cgacccctat tacgtgtaca tcaccacggc gccggaattc
      121 caaccggtca aggccccggc aaaccttgcc ctcgcccgtt gggatacctg gatttacatg
      181 ccctggcgct accaatggac aattggcacc ggtgatgaag gcggccagtt ttgccgcgat
      241 ttcgggatca acggcggatt cacagatcac ggcgatggac ctttcgcatg gcttgagcaa
      301 tggaatctgc gattctacaa cgaccacacg gccggcaagg gcttccttta cctgcgagat
      361 gccaaccagc gatcaagatt tcaggccttt cagcgggatc cccgcgccat tcgtcaggac
      421 cgtgaggggc ttaaaccact tgatgaggcg ctcttcggaa aattggcaga attggtaaga
      481 acgcgggtcg gtagggtaag aaaaagtccc atgcgggtgg cctacgcgtt ggacgacgaa
      541 atctcctggg gcatctttgt cgtcccgctg ccttggcgcg tccacgatga tgacgcggct
      601 taccaggcat ggctggaccg gtattacggc ggaaaagggc caaaggccca gttcgtcacg
      661 cccgacttta tcctgcccca gctaaagggc cgcctagccg atctcgactt ttcgccactt
      721 ctcgaccgaa tcacctacaa cgactcggtt tgggcaaact tcatcggccg gttggtggaa
      781 gtggcaaacg aagtcgatcc cgaaacgccg tgcggctttg tgggaggcca gtcgccaaac
      841 atctggggcg gctacgacta tgcaaaactt atgaagaaga ttcaatttat cgaggcgtac
      901 gacctgggat catcgcaggc gattattcgg tcctttaatc cgggcaatgc cttcccccaa
      961 gtgaccacgc attttcactc cgatgcccga ggcacagcca acgatgtttg gcaaagttgg
     1021 tactattttg cccatggaaa ccgaggcatg atcggctggg tcgaaggctg gtttgacgga
     1081 acaaaaccac ggccatggct ttacgaatat cgcgccacgc tgaaagagct aggcgaaacc
     1141 caaggaccga agttgttcgg cgctcgctgg gttcatgacg ggatcgccat ctactactca
     1201 catccctcaa ttcaggtctc ttggtgtttg gacatggagg cccatggcaa aacatgggtc
     1261 aataggggga acgaccatcg cttggggaca tcccatctcg tccgcaaggc atgggaatac
     1321 cttttggttg acagcggcct tcagtattcg ttcatctctt acgatgaagt tgtccgcaat
     1381 ggtatcccgc cggagtaccg ggtgttgatc cttccggcct gctacgcatt gtccgatatt
     1441 gaggccgagc ggattaaaga gttttgcaaa cggggtggaa cggtcatcgc cgacttctgt
     1501 tgtggacttt ttgaccaaca cg
//
LOCUS       DAOPTR010001020         1521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_64721_length_1521_cov_2.264666, whole genome shotgun sequence.
ACCESSION   DAOPTR010001020 DAOPTR010000000
VERSION     DAOPTR010001020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64721_length_1521_cov_2.264666"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1521
                     /locus_tag="PKI05_15430"
     CDS             <1..>1521
                     /locus_tag="PKI05_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53645.1"
                     /translation="MIRGNYVVDNTINGLYVRIKTPAGGATKILTTFARWDDWDIVHY
                     VPENLIIAGTPGGPLMRPSSAPDVTLVDDFHLRAPAASPQLDGQYFSIFDGRTRVVFE
                     FDAGDGVVTGRVPVSYTSTSTAQQIAASLAAAINAARLARGLEVAATSSGDTVTLEQR
                     GAQLKVEGLGNREARIDARLMVDPGVVVKLQGSRIETEIGAQIIAEGRAGFEETVPGY
                     KVVFTSVLDTRYGAGGTFNTTQNTSGQQPAPGNWAGIAFAPLSEGSIDQAIIAYAGGQ
                     SAIEGGFAGFNPIEIRQAHVRIANTRFENNGANSAGDRNGRGFISPATIYVRGAQPVI
                     VNNLFINNQGHVLSIDVNALNAWEVPDWGRSTGFIDAFSQYDGNFGPLVRENRMTGNG
                     INGMQVRAGTLNGQGVWDDTDIVHVVYGEIVIPDYHHEGGLRLQSSRDKSLVIKLWGQ
                     NAGFTATGSPTEVDDRVGGILQVVGMPGYPVIFTSLADDTVGAGFDRFGNPQYDTNG"
BASE COUNT          330 a          411 c          449 g          331 t
ORIGIN      
        1 aatgatccgc ggcaattacg tcgtggacaa caccatcaac ggcctttatg tgcgtatcaa
       61 gaccccggcc ggtggtgcga caaagatact gacaaccttt gcccggtggg acgattggga
      121 cattgttcac tatgtccctg aaaatctaat catcgctgga acaccgggcg gtcccttgat
      181 gcgaccgtcg tcggctcctg atgtgacttt ggtcgatgat tttcatctcc gcgcgccggc
      241 ggcaagtccg caactggacg gccagtattt ctccatcttc gatggccgaa cacgcgttgt
      301 ctttgagttt gatgccgggg atggcgtggt cacggggcgc gtacccgtaa gttacacctc
      361 cacgagcact gctcaacaaa ttgcggcttc tttggctgct gcgattaatg cagcccgcct
      421 ggctcggggg cttgaagttg ccgccaccag tagcggagac accgttacac tcgaacagcg
      481 tggtgctcag cttaaagttg aaggcctggg aaatcgcgaa gctcgaatcg acgcccgttt
      541 gatggtggat cccggtgtcg tcgtcaaatt gcagggcagc cggatcgaaa cggagatcgg
      601 tgcccagatc atcgcggagg gtcgggctgg attcgaggag acggtgccgg gatacaaggt
      661 cgttttcacc tccgtgcttg acacccgcta tggtgccggt ggaacgttca atacgaccca
      721 aaatacaagt gggcaacaac cagcaccagg gaactgggcg ggtatcgctt ttgccccgct
      781 ttccgagggc agcattgatc aagcgattat tgcctacgcc ggtggtcaat cagccatcga
      841 gggtggtttc gccgggttta atccgatcga gattcggcag gcccatgtcc gtatcgccaa
      901 tacgcgcttt gaaaataacg gggccaattc cgcaggggac cgcaacgggc gcgggtttat
      961 cagcccggca actatttatg tccgcggtgc ccaaccggtc attgtcaaca acctgttcat
     1021 caacaatcag ggacacgtcc ttagcatcga cgtgaacgcc ctcaatgctt gggaggttcc
     1081 ggactggggc cgaagcacgg ggttcatcga tgccttctcg caatatgatg gtaactttgg
     1141 cccgctcgtc cgtgaaaacc gcatgacggg gaacggcatt aacggcatgc aggtacgtgc
     1201 cggcacgctg aacggacagg gtgtttggga cgataccgac attgtccatg ttgtttatgg
     1261 ggagattgtt atcccggatt accaccatga aggcggcctc agactgcaaa gttcacggga
     1321 taagagcctt gtgatcaaac tgtggggaca aaatgcagga tttacagcca caggcagccc
     1381 cacagaagtt gacgaccgcg tcggtggcat tctccaagtc gtgggcatgc cgggatatcc
     1441 ggtgatcttc acatcgctgg ccgacgatac cgtaggggcc ggcttcgatc gctttggtaa
     1501 tccgcaatac gatacgaacg g
//
LOCUS       DAOPTR010001021         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_64954_length_1517_cov_2.716142, whole genome shotgun sequence.
ACCESSION   DAOPTR010001021 DAOPTR010000000
VERSION     DAOPTR010001021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64954_length_1517_cov_2.716142"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..366)
                     /locus_tag="PKI05_15435"
     CDS             complement(43..366)
                     /locus_tag="PKI05_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007330016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53646.1"
                     /translation="MPLYEIETEYHIIITWADNEDEAKLVVRDSYPREAILRITRRPR
                     NSWVISKAALGMTGRTDPCNIARDCLAKAAGDKIHAIRLYMQETGVDLEKARKVIESN
                     MIMGW"
     gene            complement(409..1293)
                     /locus_tag="PKI05_15440"
     CDS             complement(409..1293)
                     /locus_tag="PKI05_15440"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOA53647.1"
                     /translation="MSPVVTATAIPGLQKRSGKVRDIYDLGEQLLLVSTDRISAFDWV
                     LPNGIPNKGQVLNLLSAFWFGILGEPNHLITADVDSMPLPPGADKETLRGRTSLVKKT
                     RVVPIECVVRGYLSGSAWKEYQRTGTVCGIKLPPGLRESEQLEEPIFTPATKAETGHD
                     ENITFDQMAQIVGTATAHQLRERSIQIFKRGAAYAAERGIIIADTKFEFGWLGDELLL
                     IDEVLTPDSSRFWPADTYEPGKPQPSFDKQFVRDWLESTKWDKNSPPPALPEDIVIKT
                     RQKYIEAYERLTGTAFVV"
BASE COUNT          345 a          407 c          385 g          380 t
ORIGIN      
        1 cacgcattcg gtgagtatca ccagactgtg tttaggtgac tgtcaccacc ccataatcat
       61 attcgattcg ataaccttgc gggctttttc caggtcaaca cccgtttcct gcatgtaaag
      121 gcgaatggca tggatcttgt cacctgccgc tttggcgagg caatctcgtg cgatgttgca
      181 gggatcggta cgacccgtca tccctagcgc ggccttggag atgacccaac tgtttcgcgg
      241 tcgccgcgtg atccttaaaa tcgcttctcg tggataactg tcacgcacga cgagcttggc
      301 ctcgtcttcg ttatcagccc atgtgatgat aatgtgatac tcggtttcaa tttcatacaa
      361 cggcattgcg acatccccaa tcacaaaagc ttctgtctat ggtctctctc acacgacaaa
      421 agctgttccc gtcagacgtt cgtaagcctc aatgtatttt tgtcgcgtct tgatcacaat
      481 atcctcggga agagccggtg gtgggctgtt cttgtcccat ttggtgcttt ctaaccaatc
      541 ccggacgaat tgcttgtcga acgagggttg gggcttgccc ggttcgtagg tatcagcagg
      601 ccaaaaccgc gaactgtcgg gcgtcagtac ctcgtcgatg agtaacagct catccccaag
      661 ccagccaaat tcgaatttcg tgtcggcgat aatgataccc cgctcagcgg cgtatgccgc
      721 accgcgcttg aatatctgga tactccgttc gcgaagttgg tgggctgtgg ctgtgcccac
      781 aatctgagcc atttgatcga acgtaatgtt ctcgtcgtga cccgtctcgg ctttggtggc
      841 gggagtgaaa atgggttcct ccagttgctc gctttcccgc aatccaggtg gaagcttgat
      901 tccgcaaaca gtgcccgttc gctggtactc tttccatgcc gagccggaaa gatagccgcg
      961 cacgacacat tcaataggaa caacgcgcgt ctttttgaca aggctcgtcc gaccccgcag
     1021 tgtctccttg tcagccccgg gaggaagggg catcgaatca acgtcagccg ttatcagatg
     1081 attaggttca cccaaaatgc caaaccaaaa ggccgaaagc agattaagca cctggccttt
     1141 gttcgggatc ccgttaggca ggacccaatc aaaagcagag attcgatcag tgctgaccaa
     1201 gaggagttgt tcgccaagat cataaatatc acggacctta ccactccgtt tttgcaagcc
     1261 tggaatcgct gttgctgtaa ccacaggact catcggtgat ctttggctcc gtttggctca
     1321 gatgtgtttg agcgttacgc tcggcatatt gggatttccg attatttgaa cggggataag
     1381 gccaattcta accgaggtgc gtagataagt cgactggtga aagctatccc aaactagtgg
     1441 caaatgcacc tgtgccgcga ttgcggtgac caatgccaat caagcggcat aaagacaaga
     1501 aatgctacca cctgccg
//
LOCUS       DAOPTR010001022         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_64959_length_1517_cov_2.606019, whole genome shotgun sequence.
ACCESSION   DAOPTR010001022 DAOPTR010000000
VERSION     DAOPTR010001022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64959_length_1517_cov_2.606019"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PKI05_15445"
     CDS             complement(<1..552)
                     /locus_tag="PKI05_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53648.1"
                     /translation="MMIRAVLRKAVRDGLPQLVVSCVLLFLFSWLFVWLTSLINVGLW
                     ATFFDMLPAFVQKLMPVSPKILITPTGRVSFLFVHIVPLLIIIGWSIGRASQIVSGEI
                     ASGRYEVLLTLPVHRYLWVVVPAVVISLGAILMAGSMRLGLWVATMTVRLPERLPLAQ
                     FTPGLVNVAALSIALSGITAAVSA"
     gene            complement(658..>1517)
                     /locus_tag="PKI05_15450"
     CDS             complement(658..>1517)
                     /locus_tag="PKI05_15450"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA53649.1"
                     /translation="RDRPFFLYYATTVPHLALQVPRDSIQEYLGQFPETPYLGDRGYL
                     PCFAPRATYAAMVSRLDRDVGKILEEIKQLGLDSDTLVIFTSDNGPTHGRGGGLEDGV
                     GGSDSLFFQSAGPLNGLKGSVFEGGLRVPMIARWLGKIPPGTVSDHVAVFYDFLPTFA
                     EIVSADVPEEIDGISFLPTLLGRPEQQKTHDFLVWEFYGYGGQQAVRFGPWKAIRRDC
                     YKSLDGPLMLFNLENDPGETRDVSSEHPELVQKAELIIKAEHRDSPFWDFPTKRRGPP
                     KTLDLQTTQ"
BASE COUNT          398 a          439 c          391 g          289 t
ORIGIN      
        1 ggccgaaacc gccgccgtaa ttccggaaag cgcaatagag agtgccgcca cattgaccag
       61 cccaggagta aactgcgcaa gcgggagtct ctccggtagt cgcaccgtca tcgttgcaac
      121 ccacaggcca agccgcatgc ttcctgccat gaggatggcc cccagcgaaa tcaccacggc
      181 cggtacaacg acccaaaggt agcggtgaac gggcagtgtc aacaagactt cataccgccc
      241 tgaagcaatc tccccactga caatctgaga tgcgcgccca atggaccacc caataatgat
      301 gagtagcggg acaatgtgaa cgaacaaaaa actcacgcga cctgtgggcg ttatcagaat
      361 ctttggcgaa accggcatca gtttttgcac aaacgccggt agcatgtcga agaacgttgc
      421 ccaaagacct acattgataa gactggtcag ccatacgaac agccaggaaa aaagaaacag
      481 caggacgcaa ctcacaacga gttgcggcag cccatcacga accgccttgc ggaggacagc
      541 acgtatcatc acggttttgt gggaaaccct agttgactga tatactcacg gcattcacgc
      601 aaaagcactt cccatgaacc ggcccgtcac cccgatcagg cctcaacgct ttaaccgtca
      661 ctgggtagtt tgcaggtcaa gcgttttggg aggaccacgc cgctttgtgg gaaaatccca
      721 gaatggggaa tcacgatgct ctgccttgat gatcaactca gctttctgca caagttcagg
      781 atgttccgat gagacatccc gcgtttctcc cggatcattc tccaggttga acagcattaa
      841 gggcccatcg agcgacttgt agcagtcccg tcggatggcc ttccatgggc cgaagcgaac
      901 ggcttgttgt cctccatagc catagaactc ccagaccaag aaatcatgtg tcttctgctg
      961 ctcaggccgc cctaacaggg tgggaaggaa gctaataccg tcgatctcct ctggaacatc
     1021 cgccgagaca atctccgcaa atgttggcag gaaatcgtag aaaacggcga catgatctga
     1081 gaccgtcccc ggtgggatct ttcccagcca acgtgcaatc atgggaacgc gaagccctcc
     1141 ttcaaacacg ctgcccttga gcccatttaa tggaccagca ctttggaaaa agagcgaatc
     1201 cgagcctccc acgccgtctt ccaagccgcc acctcggccg tgggttggac cgttatcgga
     1261 ggtaaaaatc accaaggtgt cggaatccag gcccaactgc ttgatttcct cgaggatttt
     1321 ccctacgtcg cggtcaagtc gtgacaccat ggcagcgtat gtcgctcgcg gggcgaaaca
     1381 gggtaagtag ccacgatcac caagatacgg cgtctcggga aactgcccaa ggtattcttg
     1441 gatcgaatct cgaggcacct gtaacgccaa atgtggcacc gtggtcgcgt aataaagaaa
     1501 gaaggggcga tcacggt
//
LOCUS       DAOPTR010001023         1515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_65089_length_1515_cov_2.328082, whole genome shotgun sequence.
ACCESSION   DAOPTR010001023 DAOPTR010000000
VERSION     DAOPTR010001023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65089_length_1515_cov_2.328082"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="PKI05_15455"
     CDS             <1..706
                     /locus_tag="PKI05_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53650.1"
                     /translation="ALHCDTVGGTLISADYPGVLAKRLAEYKGPQFVTLFGNGTCGDI
                     NHINVHWKDRQKGPEEAGRIGTILAGDVMKALMKLQAVTDNRLAVRREIVPLPLAPIT
                     EEDVKRAKEIIAQGSKAKFLEQVFAYKVLDVADRNGQPLEAEVQVITLGRDVAWVALP
                     GEIFVDLGLSIKAVSPFRQTFVVELANGSLGYIPNRSAYAEGQYEVVSARCAEGSGEM
                     LVNAAQRLLAEVYGGE"
     gene            1097..>1515
                     /locus_tag="PKI05_15460"
     CDS             1097..>1515
                     /locus_tag="PKI05_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HOA53651.1"
                     /translation="MATVDAETASVRAAEEEPPSSGAALNVLPLFETSESGLLPYPTF
                     SGAESSSISVPPPTFLRRVRTDIRVRQFRRTFYRNVSDKLWNDWHWQISHRIRTLEEI
                     ERILVLSSDERAALEQAGAKLPVGITPYYMALISPDNP"
BASE COUNT          320 a          405 c          475 g          315 t
ORIGIN      
        1 tgcactgcac tgcgacacgg taggaggaac gctgatctcg gccgattacc ccggcgtgct
       61 ggcgaaacgc ctagcggagt acaaaggccc gcaatttgta acgctgttcg gcaacgggac
      121 atgcggtgac atcaaccata tcaacgtcca ctggaaagat cggcaaaaag ggccggagga
      181 agcgggacgg attgggacaa tcctggccgg ggatgtaatg aaggcattga tgaaattgca
      241 ggcggtcact gataaccggc tggctgttcg gcgagaaatc gtgcctctgc ctttggcacc
      301 tatcacagag gaggacgtaa aacgggccaa ggagattatc gcccagggta gcaaagccaa
      361 gttcctggag caggtcttcg cctacaaagt tctggacgtg gccgatcgca acggtcagcc
      421 gctggaggcc gaggtccaag tgatcacctt ggggcgtgac gtggcctggg tggcgttgcc
      481 gggggagatt ttcgttgacc tgggactcag cattaaagcg gtgtcgccat tccgacagac
      541 gtttgtcgtg gagttggcca acggttcgct tggatatatt ccgaatcgct cggcttatgc
      601 tgagggtcag tacgaggtgg tgagtgcccg ctgcgccgaa ggctccggtg aaatgttggt
      661 caatgcggcc cagcggctgc ttgcggaggt ctatggaggc gaataagagc ctattccaga
      721 attccctctc cctgtgggag agggttgggg tgagggtaag ccatcgtcgg agtcaatcga
      781 gaaaactagc agcgattcga gccaccctca tccctagccc ctgtcccgtc gaacgggcga
      841 ggggaggttg tgggataggc tctaaaagag gcacggatcg gctgttcttg tggcgtcgcg
      901 agatgtgcga acggcggtgg gctaaaaatc tgcatggcgg cgaaatttct tcctcttgac
      961 aagatttagt cttccgataa tccttttatg aattggcttg atcgctgtgg tatcaaggcc
     1021 gtcccgcgag tcggagcacg ggcaacgtgg atgggttgtc gcctaaccgc agcgattcac
     1081 gaaggatcgg gtagcaatgg ccaccgttga cgctgagaca gcgtctgtgc gtgccgccga
     1141 agaggagcct ccgagttccg gtgcggcact gaatgttctc cccctttttg agacctccga
     1201 aagcggtctc ctgccttacc cgacgttttc gggtgcagag tcgtcctcaa tctcggttcc
     1261 accgccgacc ttcttacggc gggtcaggac cgacatccgt gttcggcagt tccgccgaac
     1321 gttctaccgc aacgtgtccg acaagctctg gaacgattgg cactggcaaa tcagccaccg
     1381 gatccgcaca ctggaagaga ttgaacgcat cctcgtcctc tcatcggatg aacgggccgc
     1441 cctggaacag gcgggagcca aattgccggt gggaatcacg ccctactaca tggcgctcat
     1501 ctccccggat aaccc
//
LOCUS       DAOPTR010001024         1513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_65179_length_1513_cov_2.926612, whole genome shotgun sequence.
ACCESSION   DAOPTR010001024 DAOPTR010000000
VERSION     DAOPTR010001024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65179_length_1513_cov_2.926612"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(718..1293)
                     /locus_tag="PKI05_15465"
     CDS             complement(718..1293)
                     /locus_tag="PKI05_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53652.1"
                     /translation="MLRKLGYLPVLGVLGTIAVFTVGCGTVMSPLDWGVLNYPIPVSP
                     YFQDKLEDKAREEERYKKMPILPPLVEGAPHIALDEPSDDEVMRALEKARPLEGGLPF
                     LHEVQRSNVRIVKELIADSIDPPRVYPLVGPAQLHHVHWKCTVYFTETTKVGWPIPYT
                     VKNEDAVEVIYIDHDHLHMVGNVDGGPGSNY"
BASE COUNT          335 a          415 c          409 g          354 t
ORIGIN      
        1 cttggacaca gaaaccgtca aaccgattcc ggcggtccct gccgatcgca aaaactgacg
       61 tcgagtggaa tcggccatga ctatctcctt tccgcgagat cagaacctcg ttcagtcacc
      121 cccataccgc ctaccagttt gcgccggaca ggtatggtct gtcaatcact acgtagagga
      181 ttcggatacg gcgagctcgt cagcatcgtt cgcataaaaa ttggtcttga aatatcggat
      241 ggatttgagg atagttcccg ccatcttgaa agagcggata acctgtccgc gtgtttttgg
      301 gtggccactt ggccagtatt tgggtttggg ccaaatcccc gggcgagagt aatgggccgt
      361 tccatgaaaa gcaagatact tagaaaactc gtgattgcga gctgtgttag tgtgatgtcc
      421 ttgatgtggt gctccggttg taccacgttg gaccttcgtg gccagtcgcc ggagccaaac
      481 gatctctcca tattggctcg ccaagctcgc cccgcggacc cgacactgga acccttcaca
      541 ttcagtaaca aggcccgggc gatcgaggcc aacttaggag ttgggaacgg cacagccaca
      601 aatcgccagt tctaagcgta tctttcgcct tatcaaagga aaaagccttt cggaggtccg
      661 tttccgaaag gctttctgtt gttttgattg aattgtgcgg cttgtctaga cgttgcctca
      721 atagttgcta cccggccccc cgtccacatt tcccaccatg tggaggtgat cgtggtcaat
      781 gtagatcacc tcaactgcgt cttcgttttt tacggtgtaa ggaatgggcc aacccacttt
      841 ggtggtctct gtgaaataga cggtgcactt ccaatggaca tggtgaagct gggccggacc
      901 gacgagtgga taaactcgag gcggatcgat cgagtcggca atcaactcct tgacaatccg
      961 cacgttgctc cgctggactt catgcaagaa tggcaaacca ccctcaaggg ggcgggcctt
     1021 ttcgagtgcc cgcatgacct cgtcatcaga aggctcgtcg agagctatat gcggtgcccc
     1081 ctcaacgagg ggaggcagga tcggcatctt tttataccgt tcttcctccc tagccttgtc
     1141 ctctagtttg tcctggaagt aaggtgagac aggaatcggg taattcagaa ccccccagtc
     1201 aagaggactc atgacggtcc cacatcccac ggtgaaaacc gcaatggttc ccaggacacc
     1261 gagaactggt agatatccaa gtttccgcaa catgaatggc tcctcgaccg ttctgaatgc
     1321 ttgagcgatt taccctgttt acccggtcgc cacactcacc gctagtggcg gtgttctgga
     1381 taacgcgcag gagttcggta gccagctggc caactcgatc gttgtcgatc cagacgcgca
     1441 gagcagcgac gggacgcttg cgagcgcggt caacgcgacg cccagcctct tggcgagacg
     1501 ggtcattgtc tgg
//
LOCUS       DAOPTR010001025         1507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_65510_length_1507_cov_4.340220, whole genome shotgun sequence.
ACCESSION   DAOPTR010001025 DAOPTR010000000
VERSION     DAOPTR010001025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65510_length_1507_cov_4.340220"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PKI05_15470"
     CDS             <1..387
                     /locus_tag="PKI05_15470"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOA53653.1"
                     /translation="IERLKERVRVPVSLHICGNATPLLADMAATGADILEIDYPVAIE
                     DAAQILGSSVALWGNLDPVGLLAQADADTVYRETQALLRRVRQCGIRRFVVSSGCTLA
                     METPEENLDAFFAAARDFGPWGDKNG"
     gene            471..>1507
                     /locus_tag="PKI05_15475"
     CDS             471..>1507
                     /locus_tag="PKI05_15475"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA53654.1"
                     /translation="MATSVFGVLVHGAGWVSSQHIAAYQKNPATEIRAICDRSLERAQ
                     ARAKSAGLTNVAIYDDFEKALRHPGIDIVSICTPQHVHCENVLAAAAAGKHMVIEKPV
                     AINLTQLRMMQQAVKKAGVRTVVSFVLRWNPLFRTLKALLADGALGQPYYVETDYLSY
                     NGSWWSGWNDARTIEQGVSALLVGGCHAVDALRWFASTEQFKAADAEEVFAVSGGRRK
                     GQTREYNPLTNTWTDNAPPMEYDGLEVVLVKFTNGVVGKVSANAECIMPYRFPIRIFG
                     DRGTAFDNRVWSHKFPDQTDWVTIPTIAPDSSDVTHHPFQAEIDHFVECLQKGTESHC
                     NLDDAAKTHEIV"
BASE COUNT          331 a          417 c          438 g          321 t
ORIGIN      
        1 attgagcggc ttaaagagcg tgtccgtgtg ccagtgtcgt tacacatctg cggcaacgca
       61 acgccgctgc ttgctgacat ggccgccaca ggtgccgata tcctggaaat tgactatccc
      121 gttgccatcg aagacgcggc acagatcctg ggatcatcag tcgccctttg gggcaacctt
      181 gaccctgtgg gtttgctggc ccaggctgat gccgataccg tctatcgaga aacgcaagca
      241 cttctgcggc gtgttcggca atgcggcatt cggcggttcg tcgtcagttc gggctgcacc
      301 ctggcgatgg agacacctga agagaatctt gatgcgtttt tcgccgccgc aagagatttt
      361 ggaccttggg gagataagaa cgggtgaacg actgctgccc gccttttgct tagtatggtc
      421 atcgctttta aagatcgtga atacgaagag taagagtggg agacgctcgt atggctacct
      481 cagtatttgg tgtgcttgtc catggtgcgg gatgggtttc gagtcagcac atcgcggcct
      541 accagaagaa tcccgccaca gagattcgtg ccatttgcga ccgctctctc gagcgcgccc
      601 aagcaagggc caagagtgca ggcctaacca acgtggcaat ctatgacgat ttcgagaaag
      661 ccctccggca tccggggatc gacatcgttt cgatctgcac gcctcaacac gtccattgcg
      721 agaacgtttt ggctgccgct gccgccggca agcatatggt gatcgaaaag ccggtggcca
      781 tcaatttgac gcagctccgc atgatgcaac aggccgtcaa gaaggcaggc gtgcggacag
      841 tggtaagttt cgtcctccgc tggaacccgt tattccgcac gctgaaggcc ctcttggccg
      901 atggggcctt gggccaacct tactacgtgg agacagatta tctcagttac aacggcagtt
      961 ggtggtccgg gtggaatgat gcaagaacca tcgagcaggg agtgagcgcc ttgctggtgg
     1021 gcggatgcca cgccgtggac gccttgcggt ggttcgcctc cacggagcag ttcaaagcgg
     1081 ccgatgccga agaggtcttt gccgtctctg gtggacgccg aaaaggtcaa acccgcgagt
     1141 acaaccccct cacaaacacc tggacggaca acgccccgcc catggaatat gacggtttgg
     1201 aagtagttct agtcaaattc accaacggcg tcgtgggaaa ggtctcggca aatgcggagt
     1261 gtattatgcc ctaccggttt cctattagaa tctttggtga tcgcggtacc gcattcgata
     1321 accgcgtgtg gtcgcacaaa ttcccggacc agacagattg ggtcaccatc ccgacgattg
     1381 ccccggattc aagtgacgta acccatcacc ccttccaggc cgagatcgat cacttcgtcg
     1441 agtgcctaca aaaggggacc gaatcgcatt gcaaccttga cgacgcggca aagacgcacg
     1501 agatcgt
//
LOCUS       DAOPTR010001026         1505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_65648_length_1505_cov_3.297241, whole genome shotgun sequence.
ACCESSION   DAOPTR010001026 DAOPTR010000000
VERSION     DAOPTR010001026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65648_length_1505_cov_3.297241"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1505
                     /locus_tag="PKI05_15480"
     CDS             <1..>1505
                     /locus_tag="PKI05_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HOA53655.1"
                     /translation="GLEMINKVIRVDQQPLGQTPMSTPATYTGAFDAIRDLFASLPEA
                     RRRGFTRRRFSFNVPGGRCERCEGAGQLKIEMHFLPDVWVECDACKGRRFDAETLSVT
                     YHGKSIADVLEMSCEEALALFSNVPSIARILTILCDVGLGYLRLGQPANTLSGGEAQR
                     VKLAAELARPDTGRTIYLLDEPTTGLHFDDLKKLLDVLNRLVDLGNTVVVIEHNLDVI
                     KSADWVIDLGPEAGDYGGYVVAVGTPEDIVAYSRWWQARQTKTNGRKRDKRRATLPPT
                     AETPEPAFPWRCYTGEVLAPVLEAGPHVEIKRFIPPGKTQPTGQPTLPLEFATPETST
                     QEVKMPWEVNGREWHLTRECAVNGKPIRWNRQILELVMTTIDEIGRFSEPDWNSQNVV
                     EVRPSKKSLGWFLHAYTGDDSQLRLVFRTSARAAKAASPSIFKPTHVTQPRRANSWLS
                     DFPEPSEKLPSPVLERRVVRPWHEVEIRVTELTQLDRKKFCDLIGDLAEGF"
BASE COUNT          324 a          438 c          420 g          323 t
ORIGIN      
        1 ggcttagaaa tgatcaacaa ggtgatccgc gtcgatcaac agccgctcgg tcaaacgccc
       61 atgtcaaccc ccgccacata cacaggtgcc tttgatgcca tccgcgatct gttcgcatcc
      121 ttgccggaag cccgacgccg tgggtttacc cgtcgccgtt ttagcttcaa tgtccccggg
      181 gggcgatgcg aacggtgtga aggtgcgggc cagctcaaga tcgagatgca cttcttgccc
      241 gatgtttggg tcgagtgcga cgcctgcaaa ggacgccggt ttgatgccga aaccctctct
      301 gttacctatc atggcaaatc gatcgccgac gtcctggaga tgtcatgcga ggaagcccta
      361 gccctcttta gcaatgtccc ctcaattgcc cgcattttga ccatcttgtg cgatgtggga
      421 ctcggttacc ttcgcctggg gcaacctgcc aacaccttat cgggcggcga agcccagcgg
      481 gtgaaattgg cggcggaact ggcccgacct gacaccgggc gaaccatcta cttgctcgat
      541 gagccaacca cgggtttgca cttcgacgat ttgaaaaagc tcctcgatgt ccttaatcgc
      601 ctcgtggatt tgggaaacac cgtggtggtc atcgaacaca acctggatgt catcaagtcg
      661 gcggactggg tcattgacct tggaccggaa gccggtgatt acggcggata tgtggtggca
      721 gttggtacgc cggaagacat tgtggcctat tcccgctggt ggcaagcccg gcaaactaag
      781 accaatggtc gcaaacgtga caaacggagg gccacacttc cgccgacggc cgaaacgccg
      841 gaaccggctt tcccttggcg ttgttacaca ggcgaggtcc tggcacccgt tctcgaagct
      901 ggccctcatg tcgagatcaa gcgttttatt ccgcccggca aaacgcagcc cacagggcag
      961 ccaacacttc ccttggagtt tgccacgccg gagacctcta cccaagaggt caagatgccc
     1021 tgggaggtca acggccgaga atggcatctc acacgcgaat gtgcggtgaa cggcaagcct
     1081 attcgctgga ataggcagat actcgaactt gtgatgacta cgattgatga gatcggacgg
     1141 ttttccgagc ctgattggaa ctcccagaat gtcgttgaag tccgcccatc caagaaatcg
     1201 ctgggctggt ttcttcatgc ctacaccggc gatgacagcc aattgcgatt agtttttcgc
     1261 acctccgccc gcgctgccaa agccgccagc ccgagcattt tcaagccgac acatgtgacc
     1321 cagcctcgca gggcgaactc atggctcagc gactttcctg aaccttcgga gaagcttccg
     1381 tcgccagttt tggaaaggcg tgtcgtccgg ccttggcatg aggtcgaaat ccgtgttacg
     1441 gaactgacac agctcgatag aaagaagttt tgcgacctca ttggagactt ggccgagggc
     1501 ttttt
//
LOCUS       DAOPTR010001027         1501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_65891_length_1501_cov_3.489627, whole genome shotgun sequence.
ACCESSION   DAOPTR010001027 DAOPTR010000000
VERSION     DAOPTR010001027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65891_length_1501_cov_3.489627"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..666)
                     /locus_tag="PKI05_15485"
     CDS             complement(109..666)
                     /locus_tag="PKI05_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53656.1"
                     /translation="MIDFEVQRCTRHCAVTGRELKPGEVFYSVLIPDQQGWRRMDYSA
                     EAWHGPPDDCIAWWRTQLPTGSPKKRWAPSEVMIRWFEELADRADQADVRYVLALLMV
                     RRRILRLEGSRRANDGTEILTLFCPLNDTTYEIPAVIPSEDRLQQIEEQIAALMEGDE
                     IESLAGDGANSTPFRSEEGPKPERN"
     gene            1271..1343
                     /locus_tag="PKI05_15490"
     tRNA            1271..1343
                     /locus_tag="PKI05_15490"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1304..1306,aa:Lys,seq:ttt)
BASE COUNT          308 a          441 c          413 g          339 t
ORIGIN      
        1 ccgcaaagag gtccgtactc accggatcga agctttgaaa acggcgagca attcataaag
       61 ccatccctgg ccgctctttt atccaaaccg gccgcttgaa ggtctttctc aatttctctc
      121 tggcttcggc ccttcctcgc ttcggaaagg agtggagttg gccccatcac cagcgagtga
      181 ttcgatctcg tcgccctcca tcaaggccgc tatttgttcc tcaatttgtt gaagccggtc
      241 ttccgagggt atcacggcgg gaatttcgta agtggtatca ttcaggggac aaaaaagcgt
      301 gaggatctcg gtcccgtcgt tcgcccgccg tgaaccctcc agccgcagaa tccttcgtcg
      361 gaccatcaga agggctaaca cataacgaac gtctgcctga tccgcacggt cggccaactc
      421 ctcgaaccaa cggatcatga cttcacttgg tgcccatcgt ttcttcggac tgcccgtggg
      481 aagttgggtc cgccaccagg cgatgcaatc gtccggcgga ccgtgccaag cttcagccga
      541 gtagtccatg cgacgccaac cctgctgatc agggatgagg accgaataaa acacctcacc
      601 cggcttgagc tctcgccccg tcacagcaca gtgccgcgtg cagcgttgaa cctcaaaatc
      661 aatcacggtc tcttttccac aggtttttcg ccaacaaact ctttacacat aaacagaaag
      721 gctttttttg gaatccgctc ggctttcgga gtaacgttgc cgtcacagcc ggttttcctg
      781 cctggtcggg tgacaaggtt agcgaaaagt gcccgggatt tcaaccgcaa acgcccggct
      841 cgaagagaca cattattttt ccatatattc ccaaaaccct tttgtttcct agactcacga
      901 tagcgatgcc aagctgacga agcctacgtg gaggatcgac caggcagcca cagtagggct
      961 tcgccgtccg gggggctgac gattgggtcg ggcttccgct agaataatta agacgcgtaa
     1021 gaaagcacgg caaagccggt gaacgccatg ggatcccatg gccccaaaag gggggtgggc
     1081 cgtcttaggc ggaatcgcga aaattggcga ttatgaagtc aaacgccgcg tgtcggcgca
     1141 tttccgggtc gccggtcact gcgcctcgac gcggtgggcg ggcggtaggt tgccggttgc
     1201 ttgccggtcg gcgccgcgtt cggctcgtgg atgcgtccac ggtctatggc atgagtgcag
     1261 agtatcccac ggggctgtag ctcagacggt agagcaacgg acttttaatc cgtaggtctc
     1321 gggttcgagt cccgacagcc tcactcaatc tggcaccagt ttggcaaacc tcgcgcacgg
     1381 cgtgggggct tacgcgcgtc ttctcattct tttgcgtctc gtccatcgca ttcagctctg
     1441 gaggcgttcg ccacctctgg tttcgcggtt caggctctcg gtacaatggc cttcgacttg
     1501 a
//
LOCUS       DAOPTR010001028         1501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_65911_length_1501_cov_2.714385, whole genome shotgun sequence.
ACCESSION   DAOPTR010001028 DAOPTR010000000
VERSION     DAOPTR010001028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65911_length_1501_cov_2.714385"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            467..>1501
                     /locus_tag="PKI05_15495"
     CDS             467..>1501
                     /locus_tag="PKI05_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOA53657.1"
                     /translation="MTKNPITREEILRGLDGFQDPELGRSIVRLDQVQDVQISDGLVK
                     IVLGLTSFSAPLWPELREEVRNLLRTQLTPDVDWEVVIREFPRPAEKLGTIGLTAKYV
                     IAVGSGKGGVGKSTIAAALALGLDRAGCKVGLLDADVYGPSIPHLLGSDERPTMYEGK
                     IIPITVGNLRVMSMGFIVPKDEAVIWRGPMLHGAVQQFLRDTDWGDLDYLVVDMPPGT
                     GDVAISLSQLLPVSGAVVVCTPQELALLDAVKAITMFRKVHIDVLGIVENMSYFICPN
                     CGTRHDIFSSGGARKKADELGLPFLGEVPLNVMLRIKGDEGRLVECLAEENISPYFDT
                     ICKSVVRQIVN"
BASE COUNT          339 a          370 c          432 g          360 t
ORIGIN      
        1 gaatacggtg cgcattgcgt ggtgtcgtcg cctcgagagc tcaaaaaggt ggcgatctgg
       61 ctcgcctggc atctgcgacg gttaccacct attttgaaca atccacgggc tcagatttgg
      121 gaccgacttc cttggtcgca gtgggattca aggaaaaata tggcgtcttc aagttgactg
      181 cgcacacatg agataatccc cacgggtaac tagcctcacc aaaactatct ctcagaaaat
      241 gcgtaggatc agtagctcag gaaaggtacc atcactccca ctcactctca tccgcgggag
      301 ttgcccctcc tgggcttcgc tgggcaatac ttggtggaaa gcgtgccgtt ctgttgcatt
      361 ttccgggaag aacagcaaaa gcccggcgtg gtgagcggtg ctctgtgaac gggacatact
      421 cggtggatga gacggtaatt caaactaaca aggagaaata taaatcatga caaaaaaccc
      481 aataacccgc gaggaaatcc tcaggggcct cgatggcttt caggaccccg aattgggccg
      541 tagcatcgtt cgtttggatc aggtgcagga cgtacaaatc tccgacggtt tggtcaaaat
      601 cgtcttggga ctcacgtcat tttctgcccc tctatggccg gaattgcggg aggaagttcg
      661 taacctgttg cgaacgcaat tgaccccgga cgtcgactgg gaagtggtga ttagggaatt
      721 tccccggccg gccgagaagt tgggcacgat cggcctaacg gccaagtatg tgatcgcggt
      781 aggatcgggt aagggaggcg tcggaaaaag cacgattgcc gcggcgcttg ccttgggcct
      841 ggatcgggcg ggttgtaaag tgggtctact ggatgccgac gtttatggac caagcattcc
      901 ccatctcctt gggtcagatg agcgccccac catgtacgag ggtaaaatca ttcccattac
      961 cgtgggcaat ctccgcgtta tgtcgatggg ctttatcgtt cctaaggatg aggccgttat
     1021 ttggcgaggt ccgatgctcc acggagccgt gcaacaattc ttgcgagata ccgactgggg
     1081 tgatttggac tatctcgtgg ttgatatgcc accggggacg ggtgacgtgg cgattagcct
     1141 ctcccaattg cttcctgttt ccggtgccgt tgttgtttgt acaccccagg agcttgcgct
     1201 tcttgatgcg gtcaaggcca ttacgatgtt tcgcaaggtc cacatcgatg tgctcggcat
     1261 tgtggagaat atgagctact tcatctgtcc caattgtggt acgcggcacg acatctttag
     1321 ttctggaggg gccagaaaga aggcagatga gcttggcctg ccctttttgg gcgaggtccc
     1381 tctcaatgtg atgcttcgga ttaagggaga cgagggacgc cttgtggaat gcctcgccga
     1441 agaaaacatt agcccttatt tcgacacaat ttgtaaaagc gttgtacggc aaatcgtcaa
     1501 c
//
LOCUS       DAOPTR010001029         1499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_66028_length_1499_cov_2.894044, whole genome shotgun sequence.
ACCESSION   DAOPTR010001029 DAOPTR010000000
VERSION     DAOPTR010001029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66028_length_1499_cov_2.894044"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PKI05_15500"
     CDS             <1..402
                     /locus_tag="PKI05_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyvinylalcohol dehydrogenase"
                     /protein_id="HOA53658.1"
                     /translation="EGEKITPEVLYRLPPQEFGSTQQTPVFFAGCIFGVREKDKQMVC
                     LDLDGKVRWSSGSQYRFGLGPYMIANGLLLALDDDGTLTLAEASPEAFKPIARARVLD
                     GHDCWGPMAIVKGRLIIRSMTEMVCLDLTSP"
     gene            433..>1499
                     /locus_tag="PKI05_15505"
     CDS             433..>1499
                     /locus_tag="PKI05_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53659.1"
                     /translation="MKYRVPDDRHTGFSRYTSQMDRGSLPSGIQRWELLLMVVVVVAA
                     AAVGAVAVWQYRNHLRNPSRDLKAERFSYSLDLYQEIPPERIGFEPAWQLAIPGDQIR
                     RVTVFQNTAWVCADQSLIPVALTSEGGAVGSPMSVPVTPYCLAVAEWPDQDNRAERYF
                     VVGGGNQVLVIRETGAVVGEWSFFGRKANITDLAIADEDVFLADAGNRVVHRVDRFGE
                     KVAEIGHRDAERGILGFIIPSPYFSLAWNGDGLLRVVNPGVHRIEFYSPDGDLETFWG
                     KAGLDEAGFCGCCNPARIALLMDGRIVTVEKGIPRIKVYSPHGDFICWVVLPSYFTGS
                     AQWKETRDQERLPVLDVGVFQV"
BASE COUNT          303 a          374 c          460 g          362 t
ORIGIN      
        1 gaaggggaga agatcacccc ggaggttctt taccggctac cgccacagga atttgggtca
       61 acccagcaga cacctgtttt cttcgcaggg tgcatctttg gggttcgtga aaaagacaaa
      121 caaatggtct gcctcgattt ggatggaaag gtccgttggt cgagtgggtc gcaatatcgg
      181 tttggcctcg ggccgtatat gattgctaat gggttgctgt tggctttgga tgatgacggc
      241 acgttgaccc tggccgaggc gtcgccggag gctttcaagc cgatagcccg cgccagagtc
      301 ttggacggtc acgattgttg ggggccgatg gccatcgtaa aaggacgttt aatcattcga
      361 agcatgaccg aaatggtttg tcttgattta acctccccgt agccatcgta aggcctgcgc
      421 aaaggtcaat ccatgaaata tcgggttcca gacgatcgcc acaccggctt cagcagatac
      481 acgtcccaga tggaccgtgg atcattacca tccgggatcc aacggtggga actgcttcta
      541 atggttgttg tcgttgtcgc ggccgccgct gtgggcgcgg ttgcggtgtg gcagtatcgt
      601 aaccacttgc gaaacccttc ccgtgacttg aaagcagagc gattttccta ttcactcgat
      661 ctttaccagg agattccacc tgagcgaatc ggcttcgaac cggcgtggca gcttgccatt
      721 cccggagacc agatccggcg ggtgaccgtc ttccaaaaca cggcatgggt gtgtgccgat
      781 caatcgttga ttcccgtggc ccttacttcg gaaggtggcg ctgtgggatc ccccatgagc
      841 gtgccggtga caccatactg cctcgccgta gctgaatggc cggaccaaga taaccgcgcg
      901 gagcggtact ttgtcgtagg aggtgggaat caggtgctgg tcatccgaga aacaggggcc
      961 gtcgttggtg aatggagttt cttcggtcgg aaggccaata tcaccgattt ggccattgct
     1021 gatgaagacg tttttcttgc cgacgcaggt aaccgggttg tccatcgggt ggaccgtttt
     1081 ggcgagaagg ttgccgagat cggccaccgc gacgcagaac ggggaatcct agggtttatc
     1141 atcccgagcc catatttttc gcttgcctgg aatggggacg gcctccttcg cgtcgttaac
     1201 cccggcgttc atcgcattga gttctatagc cccgacgggg atctggagac tttctggggt
     1261 aaagcgggac tggatgaggc gggcttttgc ggatgctgta atccggcgag gatcgccttg
     1321 ctgatggatg ggcgaattgt cacagttgaa aagggaattc cgcgaattaa ggtttattcg
     1381 ccccacggtg acttcatctg ctgggtcgta ttaccgagct attttactgg ttcggcacaa
     1441 tggaaggaga cccgggatca agagcgatta ccggtcctcg atgtgggcgt ctttcaggt
//
LOCUS       DAOPTR010001030         1499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_66046_length_1499_cov_2.408587, whole genome shotgun sequence.
ACCESSION   DAOPTR010001030 DAOPTR010000000
VERSION     DAOPTR010001030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66046_length_1499_cov_2.408587"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..>1499
                     /locus_tag="PKI05_15510"
     CDS             141..>1499
                     /locus_tag="PKI05_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53660.1"
                     /translation="MPVTVGGPVDGGFADRYPEMQAIWRDPQALSPDEVPEGSAEWAF
                     RCAARNGRLANEMFFCANKYVQGWLEQADPKTGLIPRNLTQSRDIWNPEDAAADNYPF
                     MVLTSYLTERGLFDGRMLAMLETETRLTSRLGSIPDAWSFSKQGFVRPEPQLGALIFG
                     GSEYVKDGLLPLTEFLGPSPWLDRMLAIVDDIWKVAPVETPWGAIPSENVEVNGEQLQ
                     ILSRLFWLTGNRKYLDWAKRLGDYYLLGSHHPTRNMQNLRLRDHGCEIVSGLCELYVA
                     VSFADRAKAEEYREPIHAMCDRILEVGRDARGMLYNSINPLTGEHDPNICDTWGYNYN
                     GIYSVYLVDKTPEYREAVRFVLSQLESQVGDYHWGSADEYADSIESAINLYNREPDSS
                     AAAWIDRKIHYMWRPQQPSGIIEGWHGDGNVARTGIMYAFWKTQGLRVEPWREDVRLG
                     AVL"
BASE COUNT          311 a          374 c          452 g          362 t
ORIGIN      
        1 ccgccagatc cggggattcg ttggagtttc gtctttcggg ggcctcagac aaagcggcct
       61 tcttctcgca tgctgcttta atctgccaag gagggaagac ggagggttca cttccaaaat
      121 tcactctccg tgtgaacctg ttgccggtca cggttggtgg accagtggat ggtggttttg
      181 ctgatcggta tccggaaatg caggccattt ggcgtgatcc gcaagcactt tctcccgatg
      241 aggtccccga aggctctgct gaatgggcgt ttcgttgcgc agcgagaaat gggcggctgg
      301 ccaacgagat gtttttttgc gccaataagt acgtgcaggg ctggcttgag caggctgatc
      361 ctaaaacggg tctaatcccc agaaacctca cccaatcccg cgatatttgg aatccggagg
      421 atgccgcggc tgacaactac ccgttcatgg ttttgaccag ttatctgaca gaacgtgggc
      481 tttttgatgg ccgcatgttg gcaatgctgg agactgaaac gcgtctgacg agccgccttg
      541 gttcgattcc cgatgcatgg tcgttctcga agcagggttt cgttcggccg gagccacagc
      601 ttggggcttt gattttcggg ggctccgagt atgtgaaaga cggcttgttg cctcttacgg
      661 agttcttggg cccgtcgcca tggttggacc gaatgctcgc gattgttgat gatatatgga
      721 aagtggcccc ggttgagacg ccgtggggag caattccctc ggaaaacgtg gaggtcaacg
      781 gtgaacaact ccaaattctt tcccggttat tttggctgac gggcaacaga aagtatctgg
      841 attgggccaa gcggctaggc gattattacc tcttgggttc tcatcatccc actaggaata
      901 tgcagaatct gcgactccgc gaccacggtt gcgagattgt ctctggtctg tgcgagttgt
      961 atgtggcggt cagttttgcg gatcgggcca aggccgagga ataccgcgag cccatccatg
     1021 ccatgtgcga ccgcatcctt gaagtgggac gggatgctcg cggaatgtta tataacagca
     1081 tcaatccgct gacaggtgaa cacgatccca acatctgcga cacctggggc tacaactaca
     1141 acgggattta ttctgtgtac ctggtagata agacgccgga ataccgtgag gctgttcgct
     1201 tcgtcctttc tcagttggaa tcccaagtag gcgattacca ctggggttca gccgacgagt
     1261 acgccgatag catcgaaagt gccatcaatc tctacaatcg ggagccggat tcttcggcgg
     1321 ctgcgtggat cgaccggaag attcactaca tgtggcgtcc tcagcaaccg agtggcatca
     1381 tcgaagggtg gcatggggac ggcaatgttg cgcggacggg aattatgtac gccttttgga
     1441 agacccaagg actacgggtc gagccctggc gcgaagacgt ccgcttaggg gctgtgctt
//
LOCUS       DAOPTR010001031         1498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_66090_length_1498_cov_2.972280, whole genome shotgun sequence.
ACCESSION   DAOPTR010001031 DAOPTR010000000
VERSION     DAOPTR010001031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66090_length_1498_cov_2.972280"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..>1498
                     /locus_tag="PKI05_15515"
     CDS             59..>1498
                     /locus_tag="PKI05_15515"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA53661.1"
                     /translation="MNEWLASRLNLRPGDSLEVTWFTPETQRGQVERGKAILKVADIV
                     SLRGAADDPALVPEVQGLTDRESIADWDPPFPFDASRVTKEDEQYWEQYRATPKIFVS
                     PEFALKHWKSRFGTTTSIRVVPSAAIDVPQLIQKLTLPLSAIGWTPQPIRSQALEAAK
                     GTTPFEWLFLGFSFFVIIAALLLTGLIFALTLERRRVQIGIVRATGWSPRHVLKWLNT
                     EALFLSILAGAVGIIGGVAYAQLMLWALHTVWLPAIGTRFLTLFFRPESLITGFTFGV
                     LSGLFATLLVIIRLSRRPPSVLLTGQTTDPHSRRAHNRLRLWSLRGVTVCLFLLAALL
                     GIWGTTQGEQNQAGIFFLSGGLILLAFWLTLKLVLYHMDRSRRRIPSLAGLAIRNLSR
                     NRARTELTVLLMAAAVFIIIAISAFYVDPAELVPHFNSGNGGFTLIGETDLPVFDDLN
                     DPTVRDNLGLWPKELQMLNSARVFPFRVKS"
BASE COUNT          301 a          411 c          385 g          401 t
ORIGIN      
        1 gccgtttctt tcaaccgagg gtcgtgtcgt ttctcaaccc gctgttgacc aatgcgtcgt
       61 gaacgagtgg ctcgcgtcgc gattaaacct tcggcccggt gattcccttg aggtaacgtg
      121 gttcactcct gaaactcaac gcggtcaagt cgaacgcggt aaggcaattc tgaaggtggc
      181 cgatattgtc tctctccgag gggccgcaga tgatccggct ttggtgccgg aggttcaagg
      241 tttgaccgat cgcgagtcga ttgccgactg ggaccctcct ttcccatttg atgcgtcgcg
      301 ggttacgaag gaagatgagc aatactggga acaatatcgg gcaacaccta agatctttgt
      361 gtcacccgag ttcgctttga agcattggaa aagccgcttt ggcactacaa cgtccattcg
      421 tgtggtacct tcggcagcga tagatgtgcc acaactcatc caaaaattga cgctcccact
      481 ctcggcaatt ggctggacac cccaaccgat ccggtcgcag gcacttgagg cggccaaagg
      541 aacgacaccc tttgagtggc tttttctcgg tttcagcttt tttgtcatca tagccgcctt
      601 gctcttaacg ggccttattt ttgccctcac gctcgagaga aggcgcgtgc agatcggtat
      661 tgttcgggca acaggttggt cccctcggca tgttctgaag tggcttaata cggaggccct
      721 ttttctaagc atccttgccg gggcagtcgg aatcattggc ggtgttgcct atgcccaact
      781 tatgctttgg gcgttacaca ccgtttggct tcctgccatt gggacacgct ttttaaccct
      841 ctttttccgt ccggaatcct tgatcaccgg tttcactttt ggcgtgctca gcggtctttt
      901 cgccacgctt ttggtcatca tacgcttatc acggaggcca cccagtgtgc tcctgaccgg
      961 tcagacgacg gacccacact cgcgtcgtgc ccacaatcgc cttcgattat ggagtctgcg
     1021 tggcgtcacg gtttgcctct tcctgcttgc agcactattg ggcatttggg gaacgaccca
     1081 gggagaacag aatcaggccg gtatcttctt tcttagcggt ggcctcatat tactggcgtt
     1141 ttggctcacc ctaaagctcg tcctctatca catggaccgg tcgcgaagaa gaatccctag
     1201 cctagccgga ttggcaatac gcaatctttc tcgaaatagg gcccgtacag aattaaccgt
     1261 acttctgatg gcagcagccg tgtttatcat cattgcaatc agcgcctttt acgttgatcc
     1321 tgcagagctc gtaccccatt tcaacagcgg aaatggtggg tttaccctaa tcggcgagac
     1381 ggacttaccc gtgttcgatg atctcaatga tccgacggtt cgtgataacc ttgggctatg
     1441 gcctaaagaa ttgcaaatgc taaacagtgc gagggttttc ccctttcggg tcaaatct
//
LOCUS       DAOPTR010001032         1497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_66144_length_1497_cov_3.547850, whole genome shotgun sequence.
ACCESSION   DAOPTR010001032 DAOPTR010000000
VERSION     DAOPTR010001032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66144_length_1497_cov_3.547850"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1497
                     /locus_tag="PKI05_15520"
     CDS             <1..>1497
                     /locus_tag="PKI05_15520"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOA53662.1"
                     /translation="PILRGGRPISNWTVHQGQILKADLSRTPFHGIYFRQLFFDGKRQ
                     HLARYPNFDPTKPYTGGWAYVDGKPVPMYSEIPGENRRTLRIKREDLRNWSRPEEAEV
                     CVFPRYNWWNNILRIASLDRQNGIATLAANASYPIRPNDRYYLRNLFEELDAPGEWYL
                     DKNTWTVYFWPPGDIRAAKVYAPIMETLIEIRDASDIVIQGFTLECCEGTAVLLRNTR
                     DCLIAANTIFNVGGRCQPADAAVRVEDGSGNGVVGNDIYEVGSHAIVLTGGDRTTLRA
                     AGNYADNNYIHHTGVFYKQGVGVVLQGVGNRASHNLIHDCPRFGIQYSGNDHVIEFNH
                     IRHVNLETADTGVIYSGGRDWVSPRGTVVRYNYFHDSIGFGQQNGQWTSPHYAWGIYL
                     DDNSAEVHVYGNIVVRAIRGLIHFHCARDNLVENNIFVDGTMQQLEMNGWPDHSRHIE
                     TMTLGYEQYAKLPAWQKYVGLQKGGHPKDAIPIANNRFVRNIIYYHEP"
BASE COUNT          387 a          385 c          365 g          360 t
ORIGIN      
        1 agccaatcct ccgtggcggt cgccccattt caaactggac ggttcatcag ggacaaattc
       61 tcaaagctga tttatcgcgg actccgttcc acgggattta ttttcgccaa ctctttttcg
      121 acggaaaacg tcagcatctc gcccgatatc ccaatttcga tccaacaaaa ccttacacgg
      181 gtggatgggc ctatgtcgat ggtaaacctg tccctatgta ctcggaaatc cctggtgaaa
      241 accgacggac cctccgtatc aaacgcgaag acctccgtaa ctggagtcgt cccgaagaag
      301 cggaggtctg cgtcttccca cgatataact ggtggaacaa cattcttcgc attgcgtctc
      361 ttgatcggca aaacggaata gcgacattag ccgccaatgc ctcttacccg attcgcccaa
      421 atgaccgata ctacttgcga aacctattcg aagaactaga cgcaccgggt gaatggtacc
      481 tcgataaaaa cacgtggacc gtctatttct ggccacccgg cgatatccgc gcagcaaaag
      541 tttacgcccc tatcatggaa acactcattg aaattagaga cgcgtctgac attgttattc
      601 agggattcac tttggagtgt tgcgagggca cagccgtact ccttcgtaac acccgagatt
      661 gcttgattgc ggctaacacc atattcaacg ttggtggccg atgtcaacca gccgatgccg
      721 ccgttcgtgt tgaagacggg tcgggcaacg gcgtcgttgg caatgatatc tatgaggtcg
      781 gaagtcacgc gattgtgctc accgggggcg atcgcacaac acttcgggcg gccggaaact
      841 atgccgacaa caactacatt caccatacag gtgtgtttta caagcaggga gtcggtgtgg
      901 ttcttcaagg tgtgggaaat cgggcctcgc ataatttgat ccatgattgt ccccgatttg
      961 gaattcagta ctccggcaac gaccatgtga ttgagttcaa tcatattagg cacgttaact
     1021 tagaaaccgc ggacacgggg gttatctatt cgggtggtcg agattgggtt tcaccacgtg
     1081 gcaccgttgt ccgctataac tatttccacg acagtatcgg ttttggacaa caaaatggcc
     1141 aatggacctc gccacactat gcttggggca tttacctaga cgacaactca gcggaggttc
     1201 acgtgtatgg aaatattgtg gtccgagcaa ttagaggatt gattcatttc cattgtgctc
     1261 gagataacct tgtggaaaac aatatctttg ttgatggaac catgcaacaa ttggaaatga
     1321 atggctggcc cgatcattcc cgccatatcg aaaccatgac gttaggctac gaacagtatg
     1381 caaagctgcc ggcctggcaa aagtacgtcg ggctacaaaa agggggacat cctaaggatg
     1441 ccatccccat cgcaaacaac cgttttgttc ggaacatcat ttactaccac gagccga
//
LOCUS       DAOPTR010001033         1493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_66392_length_1493_cov_3.719750, whole genome shotgun sequence.
ACCESSION   DAOPTR010001033 DAOPTR010000000
VERSION     DAOPTR010001033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66392_length_1493_cov_3.719750"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1450)
                     /locus_tag="PKI05_15525"
     CDS             complement(<1..1450)
                     /locus_tag="PKI05_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53663.1"
                     /translation="MPWRIHFPKQQGVLHIDGVTQWEPGDPYRDLTVWTPDPTSWFDW
                     LPNRWLIFPYRRVVYSEPQREGRITFQVRNSAATARTFAVLVEFAGEPWPVEIDGPQS
                     LELKAGETREVTLRYQAGPAGETRECFIRVSPQDGSGITTYGRLTVISGKAPAESPLT
                     LPLILRPYEHENEQLGYLPDYPVASQVYFDMENRPYVSSGGVLHVWDGQRWENRNLSA
                     IVRWADGKRAVRSASALTPKIAFDRLNRVYLVAQVDGRPCLLVSRDGARTFTAYELPR
                     SEGDGRTFDVEVFTGHNTLDGPPPILRYTFLFADPKVFWRRLYRLELILAEYHGEDVV
                     FSQPVTISEKVLGHSAHSGSPSCVVSREGRVHVIWSEATDPAEKVPGAPTYVVTYDRE
                     KAALSQPAFVGYGPPANDVHNTPSITMDSRGYLHTLGGTHGAPFPYARSLVPNDSSKG
                     WTEPQSLGEGLRQTYIGLVCGPNDTLHAVFRLW"
BASE COUNT          340 a          449 c          402 g          302 t
ORIGIN      
        1 tccaaaggcg aaacaccgca tggagcgtat catttggccc acacacaagg ccaatgtacg
       61 tttgtcgcaa accctcaccg aggctttgtg gttccgtcca ccccttgctt gaatcattag
      121 gaacgagcga cctcgcatat gggaacggtg ccccgtgcgt ccctcccagt gtgtgcaaat
      181 acccccggct atccatggtg atgctcggcg tattatgaac atcgttcgct ggtggaccgt
      241 acccgacaaa tgccggctgg ctaagagcgg ccttctcgcg gtcatacgtc acaacatacg
      301 tgggagcacc cggcactttt tccgcaggat cggtcgcctc cgaccaaatg acgtggacgc
      361 ggccttcgcg ggataccacg caggagggac ttccggagtg ggccgaatga ccaaggactt
      421 tctccgaaat cgtcactggt tggctaaata ccacgtcttc cccatggtac tcggccagga
      481 tcaactccag ccgatagagg cgccgccaaa agaccttcgg gtccgcgaat aagaacgtgt
      541 agcgaaggat aggtggagga ccatccaatg tgttgtgtcc ggtgaaaacc tccacatcga
      601 atgttcgccc gtcaccttca ctacggggca actcataggc cgtgaacgtc ctagccccat
      661 cgcgggagac caacaagcat ggacgaccgt caacctgggc caccagatac acccgattga
      721 gccgatcgaa agcaattttc ggcgtcagag cgctcgcaga acgcacagcc cttttcccat
      781 cggcccagcg aacgatggca gatagattgc ggttttccca ccgctggcca tcccatacgt
      841 gcagaacgcc gcccgaactg acataaggcc ggttctccat atcgaaataa acctggctgg
      901 ccacgggata atcgggcaaa taaccgagtt gttcgttttc gtgctcatat ggtcgaagga
      961 tcaagggcag cgtcaaagga gattcggctg gggccttgcc ggaaataacc gtaaggcggc
     1021 cataggtcgt gatgcccgat ccatcctggg gacttactcg gatgaagcat tcccgagttt
     1081 ctcccgcggg cccggcctgg tagcgcaatg taacttccct cgtttcaccc gccttcagtt
     1141 ccagggactg gggaccgtca atctctacgg gccagggttc tcccgcaaac tccacaagaa
     1201 cagcgaaggt tcgagccgtt gccgcactgt tccgcacttg gaaggtgatt ctcccttccc
     1261 tttggggttc gctgtacacc acccgacgat aaggaaaaat cagccaacga ttgggaagcc
     1321 aatcgaacca gctcgttgga tcgggcgtcc agactgtcaa atcgcggtaa ggatcacctg
     1381 gttcccattg tgtcacaccg tcaatgtgta aaaccccttg ttgcttgggg aaatgaattc
     1441 gccaaggcac agcgtcccgc gaaaccttcg ccggaaagcg gtgcgtggcc tgc
//
LOCUS       DAOPTR010001034         1492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_66438_length_1492_cov_4.463466, whole genome shotgun sequence.
ACCESSION   DAOPTR010001034 DAOPTR010000000
VERSION     DAOPTR010001034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66438_length_1492_cov_4.463466"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="PKI05_15530"
     CDS             <1..859
                     /locus_tag="PKI05_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53664.1"
                     /translation="WGVGVVHFPMNRREFTETGVRLGVNPRGYADRSVPVLKITDEKD
                     QLRAVLFGCACHNTTLTGQHYVISGDYAGFAKAFIEEKLPGVMALFMIGCGGDANPYP
                     RGEIEHAKQHGQELGTEVCRVVGEKLSPVSGPLKTALAEVELPLLPPPDREKLESMAK
                     GPSHLSETAKRMIETLQRGESPPSHYKAPIAVWQFGNDLTLVAISGEVVSDFVPLIEN
                     EIGHVRLWIAGYCNEVFGYLPSAKVIREGGYETRGVGTLGFFAPEAEKVVVETVRKLS
                     EQVGRPPLP"
     gene            complement(950..>1492)
                     /locus_tag="PKI05_15535"
     CDS             complement(950..>1492)
                     /locus_tag="PKI05_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53665.1"
                     /translation="APDVLEVLTTVVAAKPLQKFEVFLASYFNGFADSFVWARPSPAA
                     SREATFMKAEQKNGVWQMFPRDELAIKLIQDGRWSRPPHPVNWAIMPFFAAPLAMRRD
                     AETGLVAVVMSRPEDCFAVATPYSGEGHRSLYFSLLGRDLEPERPVTAEVRLIIGHAL
                     IDEVIVDRYRQYAGGEKRNA"
BASE COUNT          323 a          400 c          454 g          315 t
ORIGIN      
        1 gtggggcgtt ggcgtcgtgc atttccccat gaatcgccgt gaattcacgg aaaccggtgt
       61 ccgactgggt gtcaatccca gaggttatgc cgatcgaagt gttcccgtcc taaagattac
      121 ggatgagaag gatcagttgc gtgccgtgct tttcggctgt gcatgccata atacgacgct
      181 tacgggccag cattacgtca tctcaggtga ttacgccgga tttgccaagg cctttatcga
      241 ggaaaaactg ccgggcgtga tggccctttt catgattggc tgcggcggcg atgcgaaccc
      301 gtatccgcgg ggcgaaatcg agcacgccaa gcagcatggc caggagttgg gaacagaggt
      361 gtgccgggtg gttggggaaa agctgagtcc tgtatccggg cctctgaaga cggccctcgc
      421 agaggtcgag cttccactcc ttcccccgcc cgaccgcgag aagttggaga gcatggccaa
      481 ggggccttcc catctgagcg aaacggcgaa gcgaatgata gagacgctcc agcgcggcga
      541 atctccgccg tcgcattata aggccccgat cgctgtctgg cagtttggca acgacttgac
      601 attggtggcc atatcgggtg aggttgtctc cgactttgtt ccgctgatcg aaaacgaaat
      661 cgggcatgtt cggctttgga tagcgggcta ctgcaatgag gtgttcggat atctgccctc
      721 cgcaaaggtc atccgagaag gcgggtacga aacccgcggc gttggcacac ttggcttttt
      781 cgccccagaa gccgagaaag tcgttgtaga aactgtgcgg aaattgtcgg agcaagtggg
      841 acgtcccccg ctgccatagc accgatcgag gatgattcgg ccttacgcgt gaccgatcga
      901 aacttgcatc gtgagggcga ttccttggtg gtgcatgctg atgctagggc taggcgtttc
      961 gtttttctcc accagcatac tggcggtatc ggtccacaat gacttcgtca atcagcgcat
     1021 gtcctatgat caggcggacc tccgctgtga caggtcgctc cggttctaaa tcccggccca
     1081 atagggagaa gtaaagagac cgatggccct caccggaata gggcgtggca actgcgaagc
     1141 aatcttccgg gcgggacata acaacggcga ccaagcctgt ttcggcatcg cgccgcatgg
     1201 ccaacggggc tgcgaagaat ggcatgatgg cccagttcac gggatgcgga ggacgcgacc
     1261 atcgaccatc ctgaatgagt ttaatagcca actcatctcg cggaaacatc tgccaaacgc
     1321 cgttcttctg ctcggctttc atgaaggtag cctccctact tgccgcgggg gagggccgtg
     1381 cccagacgaa gctgtctgca aagccgttaa agtaggatgc caagaagacc tcgaactttt
     1441 gcaaaggttt ggctgcgacg acagtcgtaa ggacttcaag cacatctggg gc
//
LOCUS       DAOPTR010001035         1492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_66458_length_1492_cov_2.913013, whole genome shotgun sequence.
ACCESSION   DAOPTR010001035 DAOPTR010000000
VERSION     DAOPTR010001035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66458_length_1492_cov_2.913013"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            398..1081
                     /locus_tag="PKI05_15540"
     CDS             398..1081
                     /locus_tag="PKI05_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOA53666.1"
                     /translation="MFIFDPIYLIFVSPAIILALWAQFRVRSTYAQARQIPTRLSGAE
                     TARLILDSAGLTDVGVDVTPGELTDHYSPGERIIRLSPEVYRGRSLASVGIAAHEAGH
                     ALQHAFGYAPLMITQIAYPAANFGSSLGILALLIGLGIGLTWLAWAGVYLFSAVVVFQ
                     VVNLPVEFNASRRAKQLLVERGIVPPEEMPYVNRVLNAAAWTYVAATLQAILTLLYYL
                     LLVSSRDRE"
BASE COUNT          306 a          430 c          423 g          333 t
ORIGIN      
        1 ctaccttatg gtagatcgcc ccatttgccc gtgcaagcca ccacgccctc attcgccaga
       61 cggggctagt catggaaggc cacgccccgc cttggccggg aacgaccatg gatcatccac
      121 tggcaccacg gcagacacga cggcccgcgt caatccgcat attgcgcgag tcggcccctc
      181 ccaacccccg gtagggcaga ccccgtttct gccccaacag attgacgaac cgtgggagca
      241 aacggcaaca cgtagaaccc caaattggac tggccaataa cgcaggaaga cataaaatag
      301 agtacaaagg ggttttgccc ccgaggggac aggctaggac acaaatgtgt cgaattttga
      361 acaaggttga cagaggaggg aggtcatcga gacaaatatg tttatctttg atcctattta
      421 tttgattttc gtcagtccgg cgatcatctt ggccctttgg gcgcagtttc gggtaagaag
      481 tacttatgcc caagcgcggc agattcccac ccgcttgagc ggtgccgaga ccgcccgcct
      541 aatcctcgat tccgccggtt tgaccgatgt cggtgtggat gtaacacccg gtgaattgac
      601 ggatcactac tcccccggtg aacggattat acgcctgagc cctgaggtct atcgcgggcg
      661 gagcttggcg tcggtgggca tcgctgccca tgaagcgggc cacgccctgc aacacgcctt
      721 tggttatgct ccgctcatga tcacgcagat cgcttatccg gcggccaact tcggaagtag
      781 cctcggcatt ctggccctgc ttattggatt gggcatcggc ctcacgtggc tggcttgggc
      841 cggtgtctat ctcttctcgg cggtagtcgt cttccaggtg gtcaacctgc cggtagaatt
      901 taacgccagc cgccgtgcca aacaactgct tgtggaacgc ggcatcgtcc cgcccgagga
      961 aatgccttac gtcaaccgcg ttctgaacgc tgctgcctgg acgtacgttg cggccacact
     1021 gcaagccatc ttgacgctgc tatactatct gctgctcgtc tccagccgtg atcgagagta
     1081 agtggggcgc catccacgtt aaaaactttc gttggctttc tagccttgtt tccatcggtg
     1141 gtgggccttt ggaagttatt tcgtccgtgc cgatacactt ccccgcccga gctatagcct
     1201 cagggagccg tttcgttgta ttttttgagg gcaaacggcg gacctggaaa atgacagaac
     1261 ccaagaaagc gggggtaata agccgggtcc ggcgtgtggc aaggcgatcg aggaacctgt
     1321 cctagggttt tttcactaat cagaggtcct ttaacggcca ttcgacgcag aatgcgatct
     1381 tacagggttt tcgaagacac taggcttctc tgggaaaagc ctttcgagcc tgtcccgtaa
     1441 tctcccctcg cccgctcggc gggagagggg tcgggggtga gggtggctcg aa
//
LOCUS       DAOPTR010001036         1490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_66557_length_1490_cov_4.239721, whole genome shotgun sequence.
ACCESSION   DAOPTR010001036 DAOPTR010000000
VERSION     DAOPTR010001036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66557_length_1490_cov_4.239721"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PKI05_15545"
     CDS             <1..334
                     /locus_tag="PKI05_15545"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOA53667.1"
                     /translation="RMSFDMGFRREIKKVFPNAELERIPPDHPIYHNLFDIQEVDYTP
                     RVREDFGQLNAPELEGISIDGRLAVVYSRFDLGNGWEQFPHPYSYGLKDEAALKIGSN
                     VIVYAITH"
     gene            complement(364..1374)
                     /locus_tag="PKI05_15550"
     CDS             complement(364..1374)
                     /locus_tag="PKI05_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HOA53668.1"
                     /translation="MKALVLTEYHHLEVRDVPTPKPGPGELLVRVQTCGICGSDVHGL
                     DGSTGRRKPPLIMGHEAAGVVAEVGPEVTRFRSGDRVTFDSTVYCGQCAYCLRGQINL
                     CDNRRVLGVSCDEYRRDGAFAEYVVVPERIVYRLPENVSFQQAAMIEPLSVALHAVDR
                     AAPKIGETAVIFGGGIIGLLILQVLRSAGLDQIITIEPDPHRQQMAKEFAADLVLSPE
                     QFRQTAPPPVDLAFDAVGINETVNGAVNAVAKGGRVVLVGNLQPEVPLPLQKVVTREL
                     TLLGSCASQGDYPRCLNLLAKQAVRVEPLISAVAPLEEGPYWFDQLYYRRQPLMKVIL
                     QP"
BASE COUNT          330 a          403 c          432 g          325 t
ORIGIN      
        1 acgaatgtcg ttcgatatgg gattccgccg ggaaatcaag aaagtctttc ccaatgctga
       61 gttggagcgc atcccgccag atcacccgat ttatcacaac ttgttcgaca ttcaggaggt
      121 cgattacacg cctcgggtgc gggaagattt tggccagctc aatgcgccgg agctcgaggg
      181 catcagtatt gatggccgat tggcagtcgt ttacagccga tttgatcttg ggaatggttg
      241 ggagcagttc ccgcacccat attcctacgg tctcaaagac gaggctgcac tgaagatcgg
      301 ctccaacgtc atcgtgtatg ccattactca ctgataggcg gtcaaaatcc gacgaccgaa
      361 tccctagggt tggagaataa ccttcattag cggttgccgg cgatagtaga gctggtcgaa
      421 ccaatacggc ccctcttcga gcggggcgac ggcgctgatg aggggttcaa cccttacggc
      481 ctgcttggca agaagattga ggcaccgggg ataatctcct tgagacgcgc acgagccaag
      541 tagcgtcaat tcccgggtta cgaccttttg cagcggcagg gggacttctg gctggagatt
      601 tcccaccagg actacccgcc cacctttggc aactgcgttg acagcgccgt tgaccgtctc
      661 gttaatgcct acggcgtcga acgcaaggtc gacgggcggt ggtgctgtct ggcggaattg
      721 ctccggggaa agcaccaaat ccgcagcgaa ttccttggcc atttgttggc gatgaggatc
      781 aggttcgatc gtgatgatct gatcgagtcc cgcggaacga aggacttgga gaatgagtag
      841 cccgatgatt ccgccgccaa aaatcacggc ggtttccccg attttgggag ccgcacgatc
      901 gacggcgtgc agtgctacgg acaatggctc gatcattgcc gcttgctgaa aagagacatt
      961 ctcaggaaga cggtacacaa tcctttcggg aacaacgaca tactctgcaa aggctccatc
     1021 gcgacggtac tcgtcgcagg agacaccgag gacacgccgg ttgtcgcata ggttgatttg
     1081 cccgcggagg caataggcgc attgaccgca atatacggtg gaatcgaaag ttacgcggtc
     1141 acctgatcga aatcgcgtga cctccggacc aacctcggca acgacgcccg ctgcctcgtg
     1201 gcccatgatg agcggtggtt tacgacgccc ggtgcttcca tcgagaccat gcacgtcact
     1261 cccgcaaatc ccgcaggtct gaacccgaac aaggagctcg cccggacccg gcttgggggt
     1321 tgggacatct ctgacttcca ggtggtgata ctcagtcagt acaagggctt tcacggcaaa
     1381 tgtccttatc aaaagtctta agtcagcgtg attggtactt atggctttgg cttcggcgac
     1441 gcccataaca ttgggagatc atgccgccgg agtgttgctt gtgtctctgt
//
LOCUS       DAOPTR010001037         1489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_66658_length_1489_cov_2.893305, whole genome shotgun sequence.
ACCESSION   DAOPTR010001037 DAOPTR010000000
VERSION     DAOPTR010001037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66658_length_1489_cov_2.893305"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1489
                     /locus_tag="PKI05_15555"
                     /pseudo
     CDS             2..1489
                     /locus_tag="PKI05_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008670538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
BASE COUNT          340 a          388 c          433 g          328 t
ORIGIN      
        1 gctggggtcg agcaatcgtc cgctgaaatc gttaaccgac atgatcaaag gtaagcaggg
       61 gcgcttccgg gaaaaccttt tgggtaaacg ggttgattat tcagcccgta gcgtcatcgt
      121 tgttgggcct accttaaggt tgcatcagtg tggtttgccc aagcggatcg cgttggaact
      181 ctatcaaccg ttcatcatcc gccggctgcg ggaattgcag catgccgaca cgatcaaaag
      241 cgccaagaag atgatcgagc ggcgagatga agaggtttgg gacattctgg aggaggtaat
      301 tcaaaaccat ccggtcctgc tcaaccgtgc tcccacactg caccgcatgg gcatccaagc
      361 gttcgagccg gttttggtcg agggaaacgc catcaagttg catccgctgg tgtgcaaggg
      421 ttacaacgcc gacttcgacg gtgaccaaat ggccgtccat ttgcctcttt ctgtggaggc
      481 cattgtggaa gcccatacgt tgttgatgtc cttccacaac atcttcagtc cggcccatgg
      541 taagcccatt attagtccct cccaggacgt cgtcatgggt tggtattatt tgacgttgga
      601 taccttgcct aactgtaagg gcgatgacat ggtcttctcg tccttggacg aggttgagct
      661 ggcgtacagc ctggggaagg tggcaactca tgcccgcatt cgtgtccgac tgcctgatac
      721 ggtcagggtg aagggccgcg acgatattcc accgggctcc gtgattcaaa cgaccgtcgg
      781 gcgggtcatc ttcaacagca tgctccccaa gggtatgccc ttctataacg agacgatgac
      841 ctccaagaaa ttgtccgagg tgatttctga ctgttatgag atgctggggc ggccggccac
      901 gattgacctt ttggacgaca tgaatcgtct cggtttccgc caggcgactc gaagcggttt
      961 gagctttgcc accgatgacc tcttgatgcc gtcgaataag aagaaggtga tcgagcaggc
     1021 cgagcgcgag gtgattcgga cggattccgc cttccagaag ggtgtgctca ctgaaagcga
     1081 gcgcaagaac aagattctcg acgcttggac gcaggcccgc gaaatgatca cgcgggaatt
     1141 gaaggaacaa ttggagaacg accgccgcta tcccgggtac gttaacccca tttacttgat
     1201 ggcccaatcc ggtgcccgtg gtaatatcga gcaaattcgg cagttggccg ggatgcgtgg
     1261 gttgatgacg aagccctcgg gtgaaatcat cgaaacaccg atcaaggcta acttccgcga
     1321 agggttgtcg gtcttcgaat acttcagctc gacccacggt gcccgaaaag gattggccga
     1381 caccgcgttg aaaacggcag attcgggata cttgacgcga aaactcgccg acgtggccca
     1441 aaacgtggtg gtcacgatgc acgattgtgg aaccacgcaa gggatcacc
//
LOCUS       DAOPTR010001038         1488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_66712_length_1488_cov_3.147941, whole genome shotgun sequence.
ACCESSION   DAOPTR010001038 DAOPTR010000000
VERSION     DAOPTR010001038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66712_length_1488_cov_3.147941"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="PKI05_15560"
     CDS             <1..809
                     /locus_tag="PKI05_15560"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOA53669.1"
                     /translation="DALAGCLDVERFRSALRPNTRFVSIMAANNEIGSIQPIEEIAQI
                     CRERGIWLHVDAVQALGKIPFDLRKWDVDLVSLSAHKVYGPKGIGALVVRRRNPPIRI
                     EPLVYGGGHERGLRSGTLNVPGIVGFAKAVQLAYERMNEENERLCYLRNLMFELLQEA
                     LGPSIQLNGPALHLLEHRLAGNLNVQFTGIEAQTLLLHIPEVALSAGSACDAFDPQPS
                     HVLLALGLSEAEARCSLRVGIGRFNTEDEIRRATELICRAVRELSSFYRQ"
     gene            complement(888..>1488)
                     /locus_tag="PKI05_15565"
     CDS             complement(888..>1488)
                     /locus_tag="PKI05_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53670.1"
                     /translation="TPAWDIYALGCTLYYAVTGKVPFPGGTTGDKLRAHCELRPLDPR
                     RLNPELDAAFVDVMADMMAKDPRERIASAAEVVRRLRPWAIGTYPLDETCFVRSQTHR
                     SRRKLYVARPLPMDAMSLPETKPNFSPPLIDYGSENDPTSLSESSRRTEELSAPLAGM
                     GQFTQEIRGHTLLGPLVLFLLIPAVLVGLVFLIWRLLGG"
BASE COUNT          334 a          415 c          424 g          315 t
ORIGIN      
        1 gcgatgcgct ggcaggttgc ctcgacgtgg aacgatttcg cagtgccttg cggccgaata
       61 cgcgattcgt ctccataatg gccgccaata atgagatagg ctcaattcag cctatcgaag
      121 aaatcgctca aatttgtcgc gaacggggta tttggcttca cgtcgacgcg gtgcaggcgc
      181 tgggcaagat cccgtttgat cttcgcaaat gggacgttga tctggtcagc ctttccgccc
      241 acaaggttta cgggcccaag gggattgggg cgcttgttgt acggcgtcgc aaccctccca
      301 ttcgcatcga acccctggtc tatggcggtg gtcacgagcg aggcttgcgg agtggcacgc
      361 tgaatgtccc tggcattgtg ggcttcgcca aggccgtgca actggcctat gaacgcatga
      421 atgaagaaaa cgagcgcctc tgttatctgc gaaatttgat gtttgaacta ctacaagagg
      481 ccttggggcc tagcattcag ctcaacggtc ctgcgctcca tcttttggaa caccgcttgg
      541 cgggaaatct taatgtgcaa tttacgggaa ttgaggctca aaccctgctg ctgcacatcc
      601 ccgaggtcgc cctcagtgcg ggatcggcct gcgatgcgtt cgatcctcag cccagtcacg
      661 tcctactggc actcggcctg agcgaggccg aggcccgatg tagtctccgc gtcggcatcg
      721 gccgcttcaa tacagaagac gagattcgac gagcgacgga attgatttgc cgggccgtta
      781 gggaactttc gtcgttctat cggcaatgat ttttcggcat ttgacgtcaa ggggaccgag
      841 tgattcaacc gcgagaaatt ccattcacga aacgctacga accgctatca gccgccaagc
      901 agacgccaaa tgagaaagac caatcccaca agcacggcgg ggatcagaag aaacagaacc
      961 aagggaccga gcaaggtatg tccgcgaatc tcttgagtaa actgtcccat gccggccagg
     1021 ggtgcggata gctcctcagt ccgccgagaa gactcgctta acgacgtggg atcgttctca
     1081 ctaccataat caatcagggg agggctgaaa ttgggttttg tttccggcag cgacatcgcg
     1141 tccattggga ggggtcgcgc cacgtacaat ttgcgacgac ttcgatgcgt ctgagaccgc
     1201 acaaagcatg tttcgtccag aggataagtt ccaatcgccc atgggcgcaa gcgtcgcaca
     1261 acctcggccg ctgaagcaat cctttctcgg ggatccttgg ccatcatatc ggccatcaca
     1321 tcgacaaacg ctgcatcaag ttcggggttg aggcgacgcg ggtcaagtgg ccgcaattcg
     1381 caatgagcgc ggagcttgtc gcccgtcgtt ccgcctggaa agggcacctt cccagtgacc
     1441 gcataataaa gcgtgcaccc cagggcataa atatcccaag ccggtgtc
//
LOCUS       DAOPTR010001039         1487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_66774_length_1487_cov_3.416899, whole genome shotgun sequence.
ACCESSION   DAOPTR010001039 DAOPTR010000000
VERSION     DAOPTR010001039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66774_length_1487_cov_3.416899"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            289..639
                     /locus_tag="PKI05_15570"
     CDS             289..639
                     /locus_tag="PKI05_15570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOA53671.1"
                     /translation="MFKDLASLASLLRNAQDLSERLNSVIAQLKNERLVGSAGAGLVT
                     VEVNGLAEVIRVSIDQRLIDPKDKDLLEELVAGAMRDAIQKARERHMALMTELTGGLP
                     IPGLDQVAKLFGGR"
     gene            703..>1487
                     /locus_tag="PKI05_15575"
     CDS             703..>1487
                     /locus_tag="PKI05_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA53672.1"
                     /translation="MMSRTCRVWAAASVLLVVCGTVCSSITAKETTDLPNFVIIFMDD
                     MGYADIGPFGAEGYETPNIDRMAKEGRIFTDFYVTQAVCSASRAGLVTGCYNVRVSIF
                     GALNHRAQIGLHPDEVTIAEIVKQKGYATKCIGKWHLGHHPKFLPTNQGFDEYFGLPY
                     SNDMWPFHPTAGKNYPDLPLMENDRVIDENVTHEDQNMLTTWYTERAVEFIQRNKDRP
                     FLLYLAHSMVHVPLHVSDRFRGKSQRGLFGDVMMEVDWSVGQIL"
BASE COUNT          345 a          340 c          443 g          359 t
ORIGIN      
        1 gctgcccgat ttcttaataa attccgtcgt tcgtttgccc tcaccccaac tcgctcccgg
       61 agggagaggg ggaggctttt tgggataagc tctaaagggc ttaagatcgg tcaacgtttt
      121 ggaagggttc cgccgtcggc cgacgttgac gcgaactcaa caatggagag ccaccggtct
      181 ttattttccc agagtgcgtg agcgactaga atgggtaaca acattgaaag gactaacatg
      241 actcagttgt gcgaaaccta tcggtaggaa aggagtgcaa ggttgtctgt gttcaaggat
      301 ctggcatcgt tggcctcgtt gctgcgaaat gcgcaggacc tttcggagcg attgaactcg
      361 gtgatcgctc aattgaagaa tgaaaggctc gtgggaagcg caggggccgg gttggttacc
      421 gttgaggtga acggcctggc agaagtcatc cgcgtttcga tcgaccagcg attgatcgat
      481 cccaaggata aagatttgct tgaggaactg gttgccggtg ccatgcggga cgcgatccag
      541 aaggcccggg agcgtcatat ggccttgatg acggaattga cgggtggatt gcctattcca
      601 gggcttgacc aggtggccaa actcttcggt ggccgctaga attgggtccc gtagtagaga
      661 aatgttgttt tcaccgatgg tttcaagttg atgagggagg ctatgatgag cagaacatgc
      721 cgcgtttggg cggccgcatc agtcctgctg gtggtttgcg gaacggtctg tagctccatc
      781 acggcaaagg aaaccactga tctgcccaat tttgtgatca ttttcatgga tgatatgggc
      841 tacgcggata ttggtccgtt tggagcggaa gggtacgaga ctccaaacat cgaccgcatg
      901 gccaaagagg gacggatctt cacggatttt tacgtaacgc aggctgtgtg ttcggcttcg
      961 cgggcaggcc ttgtcacagg atgttacaac gttcgtgtga gtattttcgg ggcgttgaac
     1021 catcgtgccc agattggtct ccatcctgac gaggtaacga ttgccgaaat cgtcaagcag
     1081 aagggttatg cgaccaagtg tattgggaaa tggcacctgg gacaccatcc caagtttctc
     1141 ccaacaaacc agggattcga cgagtatttt gggctgccat attcaaacga catgtggcca
     1201 tttcacccaa cagcggggaa gaattatccc gatctgccgt taatggaaaa tgaccgggtg
     1261 atcgatgaga acgtcaccca cgaggatcag aatatgctaa caacctggta cacggagcgg
     1321 gcggttgagt tcatccagcg gaacaaggat cgaccgttcc tactttattt ggcacatagt
     1381 atggttcacg ttcccctcca tgtgtctgat cgctttcgcg gcaagagtca acgcggactg
     1441 tttggcgatg tcatgatgga agttgactgg tcggtcggac agatcct
//
LOCUS       DAOPTR010001040         1486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_66856_length_1486_cov_2.399022, whole genome shotgun sequence.
ACCESSION   DAOPTR010001040 DAOPTR010000000
VERSION     DAOPTR010001040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66856_length_1486_cov_2.399022"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1469
                     /locus_tag="PKI05_15580"
     CDS             <1..1469
                     /locus_tag="PKI05_15580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOA53673.1"
                     /translation="TQAKTGDGNRTANYTNNTLNQLTGRGVPGYIEVLGVALGTNTVT
                     VNGQTASRKGEYFRKELAVDNSTSALWTNITVSATNETSVSGKRFLPKAVEQFYYDED
                     GNLTNDGRWVFTWDAENRLVKVESLTATPQDSWRRVEWEFDSKGRRIRQRTSSGSTGS
                     YVVTEDLKFLSDGWKHIADLNATNNNLIRSYAWGLDLSGGMDGAGGVGGLLFMNSVAN
                     GTHCYAYDGNGNVGALVKASDGGISARYEYGPFGEVIRASGPVADENRFQFSTKRCDR
                     TTDFVLYEYRVYVPSTGRWPNRDPIEEEGGVNLYVYVEGDPVNFVDLDGLTKVKKHAT
                     SITQQQAMHGIWGWTDLESEKERPAKLKQKRCVCVVKKKPEMEFTLLVQTARKGKQSW
                     TESAVFDGVTYPNTRVYVGSGLAKFSREHEEKRYQAWKTALSSSWPAHYEDPAGQLTA
                     PTCPELQVKIDQLRQKAFAEWPNSAEYQQMIQTRMAVS"
BASE COUNT          375 a          357 c          481 g          273 t
ORIGIN      
        1 gcacgcaggc aaagaccgga gacggcaatc ggacggccaa ttacacgaac aacaccctga
       61 accagctcac gggacgcggt gtgcccggtt acatcgaggt actgggcgtt gcattgggaa
      121 cgaacacggt gacggtgaac gggcagacgg catcccgcaa gggggagtat ttcaggaagg
      181 aactggcggt ggacaacagc acgagcgcct tgtggacgaa catcactgtg agcgcaacga
      241 acgagacatc ggtgagcggc aagcgtttcc tgccgaaggc ggtcgagcag ttctactatg
      301 acgaggacgg caacctgacg aacgacgggc gctgggtgtt cacatgggac gccgagaacc
      361 ggctggtgaa ggtggagagc ctgactgcca cgccgcagga ttcgtggagg cgtgtggaat
      421 gggaattcga cagcaaaggc cgccgcatcc ggcagaggac ctcaagcggg agcacgggca
      481 gctacgtggt gacagaggat ctgaagtttt tgagcgacgg ctggaagcac atagccgatc
      541 tcaacgcgac gaacaacaac ctcatccgct cgtatgcgtg gggcctggac ctcagcggag
      601 ggatggatgg ggcaggcggt gtaggcggat tgcttttcat gaacagtgtt gcgaacggca
      661 cacactgcta tgcgtacgac ggcaatggca acgtcggcgc gctggtcaag gccagcgacg
      721 gtggcatcag cgcccgatac gaatacggcc ccttcggcga agtgatccgt gccagcggcc
      781 cggttgccga cgagaatcga ttccagttct cgacgaagcg ctgtgatcgg acgacggatt
      841 tcgtcttgta cgaatatcgg gtttacgtcc cgagcacggg gaggtggcca aaccgcgatc
      901 ccatcgaaga agaaggaggg gtgaatttat acgtgtatgt agaaggtgac ccggtcaatt
      961 ttgtcgatct tgacggtttg acgaaagtga agaagcacgc gacctcgatt actcagcaac
     1021 aagcgatgca cggtatttgg ggatggactg acttggaatc cgaaaaggag agaccagcga
     1081 agctaaagca aaagcgctgt gtgtgtgttg ttaagaagaa gcccgaaatg gagtttacat
     1141 tgttggtaca aacagcgagg aaagggaaac agtcatggac cgaaagcgct gtgtttgacg
     1201 gtgtgacgta tccaaacact cgcgtttatg tgggttcggg cttggcgaag ttctctcggg
     1261 agcatgagga gaaacgatac caggcctgga aaacggcatt gagttcttct tggccagcac
     1321 actatgagga tccagcagga cagcttacgg cacccacatg ccctgagttg caggtgaaga
     1381 tcgaccaact tcgccagaag gctttcgcgg agtggccgaa ttctgctgaa tatcaacaaa
     1441 tgatccagac ccgaatggcg gttagttaag gcccaatctt ttcttg
//
LOCUS       DAOPTR010001041         1485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_66893_length_1485_cov_3.917483, whole genome shotgun sequence.
ACCESSION   DAOPTR010001041 DAOPTR010000000
VERSION     DAOPTR010001041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66893_length_1485_cov_3.917483"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1485
                     /locus_tag="PKI05_15585"
     CDS             <1..>1485
                     /locus_tag="PKI05_15585"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOA53674.1"
                     /translation="QHGRVADVAAYTGDPELIAAMDRIWKDVTLRKMYITGGIGARHE
                     GEAFGEPYELPNESAYCETCAAIGLVFWAYRLGLMHGDAQYADVLERALYNGVLSGVA
                     LDGEHFFYVNPLASGGNHHRQPFFGCACCPTNVVRLIPSVPGYVYAVRPPTGEKPAEL
                     FVNLYLGSTAEVELEEDNVVKVTQETRYPWEGRIVLTVTPELEREWSLRLRVPGWCRE
                     ARLAVNSEGPTPVDLTKLDRGYHLIRRVWKKGDKVTLDLAMPVERIEAHPKVVADRGR
                     VAIQRGPLVYCLETVDNGFDVRTAILAKDPQFETEFRPDLLGGIVVIRATAADGRRLT
                     AVPYHLWDHRDPGPMVVWIRQAGKSRYPDPEDPAWEGRLYRPVDPTSLTAEDSPTVME
                     AARPSASHCWSYDSVEALNDGIEPRNSNDHSIPRFTWWDHRGTQEWVEYEWDKPVALQ
                     GVRVYWFDDEPRGGHCRLPASWRILYRKGDAWVPVTSQSELPIV"
BASE COUNT          290 a          373 c          483 g          339 t
ORIGIN      
        1 ccagcacggc cgcgtcgccg atgtcgcggc ctacacggga gatcccgaac tgatcgctgc
       61 tatggaccgc atttggaagg atgtcacgct gcgcaaaatg tatatcacgg gcggaattgg
      121 tgcccgccat gaaggcgagg cctttggcga gccgtatgag cttcccaatg agtcggctta
      181 ctgcgagaca tgtgccgcga ttgggctggt cttttgggcc tatcggttag ggttgatgca
      241 tggcgacgcg caatacgccg atgtcctaga gagggcactt tataacggcg ttctttccgg
      301 tgtggcattg gatggggaac atttcttcta cgtcaatcca cttgcttcgg ggggaaacca
      361 tcatcggcaa ccgtttttcg gttgtgcctg ttgcccaacg aatgtagtaa ggcttatccc
      421 ctccgtaccg ggttatgtgt atgctgtgcg gccaccgacc ggggaaaagc ctgcggaatt
      481 gttcgttaac ctttacttgg gcagtacggc cgaggtagag cttgaagagg ataacgtggt
      541 aaaagttacc caggagacgc ggtatccttg ggagggccgc attgtcctga ccgttactcc
      601 cgaattggag agggaatggt ccttgcggct gcgcgtgcca ggatggtgcc gcgaagcccg
      661 cctggctgtc aatagtgagg ggccgacgcc tgttgacttg acgaagcttg accgcggcta
      721 tcacctcatt cgcagggtgt ggaagaaggg ggataaggtg acgttagact tggcgatgcc
      781 ggtggaacgg atcgaggccc atcccaaggt agttgccgat cgcggtcggg tggccattca
      841 gcggggacca ttggtgtatt gtctggagac tgttgataac ggattcgacg ttcgcacggc
      901 catcttggcc aaagacccac agtttgagac ggaatttcgg ccggacttgc tgggtgggat
      961 tgtggtgatt agggccacgg ctgccgacgg gcgacgtttg acggcagtgc cttatcattt
     1021 gtgggatcat cgtgatcctg ggccgatggt cgtttggatt cggcaggcag gtaaatctcg
     1081 gtatcccgat ccagaggacc ctgcttggga gggaaggctt tatcgtcccg tagatccgac
     1141 cagcttgact gccgaggact ccccgacagt catggaggca gcgcgccctt ccgcgtccca
     1201 ttgttggtct tatgattccg tggaagctct caacgacggc attgaacctc ggaactcgaa
     1261 cgaccacagt atcccgcgat tcacctggtg ggatcaccgg gggacgcagg agtgggtcga
     1321 atatgaatgg gacaagcccg tggcgcttca gggtgtgcgg gtatactggt ttgacgatga
     1381 accgcgagga gggcattgcc gtctcccggc ctcgtggcgg attctctatc gcaaaggtga
     1441 tgcatgggtc ccggtgacaa gccaatccga gttgccaatt gtcaa
//
LOCUS       DAOPTR010001042         1485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_66896_length_1485_cov_3.769930, whole genome shotgun sequence.
ACCESSION   DAOPTR010001042 DAOPTR010000000
VERSION     DAOPTR010001042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66896_length_1485_cov_3.769930"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="PKI05_15590"
     CDS             <1..784
                     /locus_tag="PKI05_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007332610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOA53675.1"
                     /translation="TGVGKTELCKALAEVLFDDENAMVRLDMSEFMEKHTVSRLIGAP
                     PGYVGYEEGGVLTEAIRRRPYAVVLLDEVEKAHRDVFNILLQVLDDGRLTDNQGHTVD
                     FTNTIIVMTSNIGSHVIQEITESGGSEKEVQEAVLQLLKQHFLPEFLNRVDEVIIFRP
                     LRRPQIKKIAEIQIRRLKQRLEERGIHLEVTNRAIEALAELGYDPAYGARPLRRVIQR
                     RIENPLAVELLKESNWEGATIKVDYVGDEFIFEKVNGQASSA"
     gene            complement(1219..>1485)
                     /locus_tag="PKI05_15595"
     CDS             complement(1219..>1485)
                     /locus_tag="PKI05_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOA53676.1"
                     /translation="LEGIWATAKRLGVTEFVPQKKYEIRDDHLALHNTAGIPAIVIID
                     FDYPYWHTQEDTPDKCSPLSLAKVGWVLLEWLSSLDVAKLPPVP"
BASE COUNT          353 a          397 c          430 g          305 t
ORIGIN      
        1 gacgggtgtc ggtaagacgg agctttgtaa agccctggcg gaggtcctct ttgatgatga
       61 gaacgccatg gttcggctcg acatgagcga gttcatggaa aaacacaccg tcagccgact
      121 catcggtgcc cctcccggct atgtgggata tgaggagggc ggcgtcctta cagaggcgat
      181 tcggcggcgt ccttacgctg ttgtcctgtt ggacgaggtg gagaaggccc atcgagatgt
      241 cttcaacatc ctgctccaag tgctggatga cggtcggctc accgacaacc agggccacac
      301 cgttgacttc acaaacacga tcatcgtgat gacgagcaac atcggtagcc acgtgatcca
      361 ggagatcacc gagtccggcg gatcggagaa ggaagttcag gaggccgtcc tccaacttct
      421 taagcaacac tttctgcccg agttcctcaa tcgcgttgat gaagtgatca tcttccggcc
      481 gttgcgaaga ccgcagatta agaagatcgc cgaaattcaa attcgtcgcc tgaaacaacg
      541 cctcgaagaa cgaggtattc atctcgaggt cacgaatcgg gctatcgaag cgttggcaga
      601 gttgggctac gaccccgcct acggagccag gccgctgcga cgcgtgatcc aacggcgaat
      661 tgaaaacccc ttggcagtgg aactgcttaa agaatccaat tgggaaggtg ccacgatcaa
      721 ggtcgattac gtaggggatg agttcatctt tgagaaggtc aacggccagg ccagctcggc
      781 ttgatggttt tcagtaacct cccctgagcg ggaacgccac ctgaaacatt ttcggcgttc
      841 tctccctcgc aagtgataac tttggtatca gggaccgcga aaaatgttgc ggcgtgaagg
      901 gccgtacggt caattgaggc gcaacccggc attaggagcc tatcccaaaa aaagtccccc
      961 cctctccctc cgggagaggg tttgggtgag ggcaagcgat cgtcggagtc gatcgagaaa
     1021 acgggcagcg attcgagcca ccctaacgcc cgacccttcc cacgccgaac gggtgagggg
     1081 agattatggg ataggctcta aatttggagg ggccatggtg ctcaattggg aaaagcgtgc
     1141 gctgtcgcgt cccatatcag acccggtggg cacccatcat gggatacagt cacgacgccg
     1201 tgtagccttc taataccctt aagggacagg cgggagtttt gcaacgtcca aagaggaaag
     1261 ccactccagc aggacccacc cgactttcgc cagactcaat ggcgagcact tgtcgggggt
     1321 atcttcctgc gtatgccagt agggatagtc aaaatcaata atcacaatgg cgggaatgcc
     1381 agcagtattg tgcaatgcca ggtgatcgtc gcggatttcg tatttctttt ggggaacgaa
     1441 ctctgtcacg ccgagccgtt tggctgtcgc ccaaatcccc tcaag
//
LOCUS       DAOPTR010001043         1485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_66904_length_1485_cov_3.276923, whole genome shotgun sequence.
ACCESSION   DAOPTR010001043 DAOPTR010000000
VERSION     DAOPTR010001043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66904_length_1485_cov_3.276923"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /locus_tag="PKI05_15600"
     CDS             complement(<1..1334)
                     /locus_tag="PKI05_15600"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOA53677.1"
                     /translation="MPWPKPGEPEDWTAVDFACREVIEANPNALLIPRIPMDPPAWWI
                     QSHPDHAMTWDRPGQTRVPASVASPLYRDEAAHRLTRLVSYLETRWGDHMAGYHPCGQ
                     NTGEWFYENTWGAALSDYSPVTAQAWRQWLAKKYGNDEALQKAWENPNVQLATLDLPT
                     PDRRRSNPSGILHRFKTEQDLIDLAEFQQDMMAECVCHFAKAVREASRGQKLVVFFYG
                     YTFEFGAIQNGASTSGHYALAKVLRSPDIDILCSPISYWDRGIGGSAPAMSAAESVML
                     AGKLWLFEDDTRTYLAKESRAPGWMDGADTLHETQWLLMRNTAQVALRHFGTWWMDLG
                     ATGWFDDPELWSVMRQLQPLTEFMLSRGRPFEPEVAAIVDEKSILHVGYGGNVLTRLL
                     IYEIRRPLGRMGTPYGQYLLEDVINQRVPAKMLVFLAAWQLSKNERHELRKTT"
BASE COUNT          325 a          418 c          403 g          339 t
ORIGIN      
        1 gtcgttttgc gcagttcgtg acgctcgttt ttggaaagtt gccacgcggc caaaaacacg
       61 agcattttgg cggggacccg ttggttgata acgtcttcca aaagatactg cccgtaaggc
      121 gttcccattc ggcctagggg ccgacgaatc tcgtaaatga gcagccgcgt aagcacatta
      181 ccgccatacc cgacgtggag gatgcttttt tcatcgacaa tggcggcaac ctctggttca
      241 aaggggcgcc ctcgcgaaag cataaactcg gtcagcggct gaagctgtct cataacgctc
      301 cacagctcag ggtcgtcgaa ccagcccgtc gcaccgaggt ccatccacca cgttccaaaa
      361 tgtctcaggg caacctgggc cgtgttacgc attaacaacc actgagtttc gtgaagcgta
      421 tccgcgccgt ccatccatcc tggtgctcga ctttccttgg ccaaatatgt ccgcgtgtca
      481 tcctcaaata gccacaactt gcctgccagc atgacgcttt ctgctgcgga catcgccggc
      541 gcgcttcctc caattccgcg atcccagtat gaaatgggcg aacataagat gtcgatgtcc
      601 ggcgacctaa ggactttagc aagtgcatag tggcccgacg tcgatgctcc gttctggatc
      661 gctccgaatt caaacgtgta tccgtaaaag aaaacgacca atttttgtcc gcgggatgcc
      721 tccctgacag ctttggcaaa atgacaaaca cactcggcca tcatgtcctg ctggaactca
      781 gccaagtcaa tcaggtcttg ttctgtctta aaacgatgta aaatgcccga cggattagag
      841 cgtctgcgat cgggtgtcgg aagatcaagt gtcgccaatt gcacgttggg attttcccaa
      901 gccttttgca aggcttcatc attgccatat tttttggcca accattgtct ccacgcctgc
      961 gctgtgacag gtgaataatc actgagagcg gctccccaag tgttttcgta gaaccattca
     1021 ccggtgtttt gaccacaagg gtgatagccc gccatgtgat cgccccagcg tgtttccaga
     1081 tacgacacga gtcgggtgag ccggtgggcc gcttcatctc gatagagagg tgatgcgacg
     1141 gacgcaggaa cgcgcgtttg accgggccga tcccaagtca tggcgtggtc tggatgcgat
     1201 tgaatccacc aggcgggtgg gtccatcgga atccggggaa tgagcagtgc attggggttg
     1261 gcttcgatca cttctcggca ggcaaaatcc acggctgtcc agtcttctgg ttcgcctggt
     1321 tttggccacg gcattggcac gggaaagctg accaggttca cgccagattg agcagcaaga
     1381 cgtacctgtg accgaaacgg tccgggcggg ccgcccggac cgtttcggtc acaggtacgt
     1441 catgctgctc aatctggcgt gaacctggtc agcaagacgt acctg
//
LOCUS       DAOPTR010001044         1482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_67077_length_1482_cov_4.493343, whole genome shotgun sequence.
ACCESSION   DAOPTR010001044 DAOPTR010000000
VERSION     DAOPTR010001044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67077_length_1482_cov_4.493343"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PKI05_15605"
     CDS             <1..331
                     /locus_tag="PKI05_15605"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53678.1"
                     /translation="GALVDGCVRDTNALSRFSFPIFSRGAFAQDQRTRGKVVDFRVAV
                     QIGNVRVRPGDIIFGDHEGVCILPQGSEGDVVEAALQRLQKRAEARRELESGSTAVEV
                     FAKHNMI"
     gene            448..>1482
                     /locus_tag="PKI05_15610"
     CDS             448..>1482
                     /locus_tag="PKI05_15610"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOA53679.1"
                     /translation="MIVTEERDRIRGKALKAGGTIMLTVLFVLLGTITVSEPANEWVV
                     LPGHSQLADPQSMMTTYLLGKIAEAEKRWREAYEKRTNLEEIKAHQAFLKEEFLRRIG
                     GLPERTPLNSQIVGRLERRGYRVEKVIFESQPKHFVTAALFLPDPNKFKPPYAAVLVP
                     CGHSNEAKAYRNYAGFGALCAFNGLAALVFDPIDQGERHQWRDPDGKFTLWGTAGHNM
                     IGVGSILLGRNTARYEIWDGMRAIDYLQSRPDIDPDKIGCSGISGGGTQTAYLMALDP
                     RIKAAAPGCYICSLYGRLIKTNGPQDAEQNIYGQLALGMDHADYCMMRAPAPTLLLTA
                     TRDYFDIEDAW"
BASE COUNT          324 a          389 c          449 g          320 t
ORIGIN      
        1 cggggcctta gtcgatgggt gcgtccggga tacgaatgcc ctcagccgtt tttccttccc
       61 catcttttct cgtggtgcct ttgcccagga ccagcggaca cgtggaaaag tcgttgactt
      121 tcgcgtggcc gtgcaaatcg gcaatgtgcg ggtacggccg ggtgatatca tctttggcga
      181 tcacgaaggg gtgtgtatcc tcccgcaagg ttcggagggt gacgtggttg aggcggcatt
      241 gcagcgacta caaaagcgag cggaggcccg ccgcgaactc gaatctggtt ccaccgctgt
      301 ggaagttttc gccaaacaca atatgatata gggcgaatga cggaggcgtg atcctgcgca
      361 ggaggcggtc gggcattgcc cgagatgatc gccgcatcca accctctgtg gaggctggat
      421 cgtcatacca aaggattgtg tgtccggatg attgtcacgg aagaaaggga tcgtattcgc
      481 ggaaaggcct tgaaggccgg ggggacgatc atgctcactg tactgtttgt cctgctgggt
      541 accatcacgg ttagtgaacc tgccaacgag tgggtggttt tgccgggtca ttcgcagctt
      601 gccgaccccc aatccatgat gacgacgtat ctgctgggga aaattgccga ggcagaaaag
      661 cggtggcggg aggcctacga aaaacgcaca aatctggaag agatcaaagc gcatcaggcc
      721 ttcttaaagg aggagttcct gcggagaatt ggtggcctgc cggagcgaac accgctcaat
      781 tctcaaattg ttggccgact tgagcgccgc ggttaccgag tggaaaaagt gatcttcgaa
      841 agccaaccga agcattttgt gacggcggcg ctctttttgc cggatcccaa caagttcaaa
      901 ccaccgtatg cagccgtttt ggttccgtgt gggcattcca acgaagccaa ggcctatcgc
      961 aactacgcgg gtttcggggc cttgtgcgcg tttaatgggc tggcagcatt ggtctttgat
     1021 cccatcgacc agggagaacg tcatcagtgg cgcgatcctg atggaaagtt caccctatgg
     1081 ggaacagccg gacataatat gatcggcgtc ggttccattc ttttgggccg caacaccgca
     1141 cgctacgaaa tctgggatgg catgcgtgcc atcgactatc tgcaatcgcg gcccgatatc
     1201 gatccagaca agatcggttg ctcgggaatt agtggcggcg ggacgcagac ggcctacctc
     1261 atggcacttg atccccgcat caaagcggct gccccgggct gctacatttg cagtctctat
     1321 ggtcgactga tcaagaccaa cggacctcaa gacgcggagc agaatatcta cggacaattg
     1381 gccctgggga tggatcatgc cgactactgc atgatgcggg caccggcccc gacccttctt
     1441 ttgacggcga ctagagacta cttcgacatc gaagatgcct gg
//
LOCUS       DAOPTR010001045         1478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_67372_length_1478_cov_2.467323, whole genome shotgun sequence.
ACCESSION   DAOPTR010001045 DAOPTR010000000
VERSION     DAOPTR010001045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67372_length_1478_cov_2.467323"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            566..1465
                     /locus_tag="PKI05_15615"
     CDS             566..1465
                     /locus_tag="PKI05_15615"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOA53680.1"
                     /translation="MADQVVDIAVVPVAGRGTRLLPMTKSQPKEMLPVGRKPVVQYVV
                     EELVRCGIKRFLFITGPGKTAIENHFDLNPELIQMLRETGKEELLRELAFERAAVDYF
                     YTRQRRQLGLGHAVLCAEPMVDGQPFVVALGDSIIGLQAQSDIVARMIAVFETEKADA
                     VIAFETVPREDVIYYGIAEPEKNWDTNGHFFALKGIIEKPSPETAPSCLAVAARYVFS
                     PVIFTHLKHTKPGKGEEIQLTDAIQRLIESGGKVLGMRLPEGERRFDIGNFESYFRAF
                     VEFALADEEYGEGLREYIKHLMK"
BASE COUNT          343 a          357 c          428 g          350 t
ORIGIN      
        1 cctataaatt gcagcaccac cggcgattca tcaattacga agaattgctg accgtcatca
       61 aatcacttgg gtatcacaag taacccgtgc tccccgggga attgctgtgc gtaacccatc
      121 ccttgtctat tagagcctat ccctaaaagt tccccctctc cctccgggag agggttgggg
      181 tgagggcaag tgaacgtcgc aatcagttga gaaaaccagc agcgattgga gccaccctta
      241 cgcccgaccc gtctctcgcg gaagcggtga aaggagatta tgggatacgc tcttagcaaa
      301 gcggggtcag tttgccggca ctccgcgaag ccattctgtg agtgatcact cgtcttcgat
      361 ttgccgtcac cgatcattct gcctcgtgag aagttatagt taagtagtag gatcggtgac
      421 cggcgtcggc gaggggtttt tctgaagacc agcccgcgat cgttcagaac gtcgagctgt
      481 tagattggca gcgggggctc caaaccattc tgggaagccc ttcgcgattg cgcgtgagga
      541 ttcaataaca ggaaaaggtt tttctatggc agatcaggtt gttgatattg cggtggttcc
      601 cgtggcgggg cgagggacac ggcttctccc gatgacgaaa agccagccca aggagatgct
      661 tccggttggt cgaaagccgg ttgttcaata cgtggtggag gaattagtcc gttgtgggat
      721 caagcgattt ctctttatta cgggccccgg gaagacggca atcgagaatc acttcgatct
      781 taacccggag ttgattcaga tgctgcggga gaccggcaaa gaggagttgc ttcgcgaatt
      841 ggcctttgaa cgggcggccg tggattattt ttatacccgc cagaggcggc aattgggact
      901 gggacatgcc gttttgtgtg cggaaccgat ggtggacggc caaccatttg tcgtcgccct
      961 cggcgattct attatcggtc tccaagccca atctgacatt gttgcaagga tgattgcggt
     1021 tttcgagaca gagaaggccg atgcagtcat tgcatttgag acggtgccac gcgaagacgt
     1081 catctactat ggtatcgcgg agcctgagaa aaactgggat accaacgggc atttctttgc
     1141 cttaaaaggg atcattgaaa agccatctcc cgaaacggcg ccgagttgcc tggccgtcgc
     1201 cgcccgatat gtcttttccc ccgtgatttt tacccatttg aaacacacta aacccggcaa
     1261 aggagaggag attcagctga ccgatgcgat tcaacggtta attgaatcgg gaggaaaggt
     1321 cttggggatg cgactaccag agggcgagcg acgtttcgac attgggaact tcgagagcta
     1381 ttttcgggcc tttgtggaat ttgcgttggc ggacgaagaa tacggcgagg ggttgcgcga
     1441 atacattaag cacctcatga agtgaaactg tcccctca
//
LOCUS       DAOPTR010001046         1477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_67418_length_1477_cov_3.335443, whole genome shotgun sequence.
ACCESSION   DAOPTR010001046 DAOPTR010000000
VERSION     DAOPTR010001046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67418_length_1477_cov_3.335443"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            241..780
                     /locus_tag="PKI05_15620"
     CDS             241..780
                     /locus_tag="PKI05_15620"
                     /inference="COORDINATES: protein motif:HMM:NF022061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53681.1"
                     /translation="MKLGYLCLVSVISVALVSGFAWAEPGKKAKKGEKAPPTPEELFA
                     QMDTNNDKAVNLDELLAMKQVKGDKEFAQKLLALWDENKDGKATKDEFNKFLESQAQQ
                     REKQFAKVDLNADKSLSLDEIKSMKQVGGDETKAKAFLERLDDDKDGKISVKEFTRVR
                     AIFQLPPQEKEKKEKKGAN"
     gene            complement(1005..>1477)
                     /locus_tag="PKI05_15625"
     CDS             complement(1005..>1477)
                     /locus_tag="PKI05_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53682.1"
                     /translation="PYSEVCKVEMRLPGAYCHRVFETHMVPYFAQFPIVEIDRSSAQF
                     WASMLYRSDFARYGDQWDWRARLSYATYLAGLRNGLIVPKPQLHPAGAGSRTECPPGE
                     RPHPVVVLNLFCGEKTEGTMDRVIAPPLLEPKPTVRITTSSAVLPMRVTNPFIP"
BASE COUNT          370 a          335 c          441 g          331 t
ORIGIN      
        1 tcatactact ctctagaggg ggtgacagcc gtggcgacaa gttgggtatg acatgcgggg
       61 gattcgcagc aagttcaaag ctgcgcttag gtgcgccgcg caattcataa gttaaggacg
      121 cgggtcccaa gtcttttaag gttagtggtt tctagacaga gaagagtgtt ggttattcgg
      181 ccgcatcggt tgcggtcgat tggggattaa gggtcggctc cttttgtgga ggtgttacct
      241 atgaagctcg gttacctgtg tttggtgtca gttatctctg ttgccctggt gtcgggattt
      301 gcttgggccg aaccaggcaa gaaagcgaag aagggcgaga aggctccgcc cacgccggaa
      361 gaattgttcg ctcaaatgga tacgaacaat gacaaggcgg ttaatttgga cgagcttttg
      421 gccatgaagc aggtgaaagg agataaagag tttgcccaaa agcttctagc gttatgggat
      481 gaaaacaagg atggcaaggc caccaaggac gagtttaaca agtttctgga atcccaagcg
      541 caacagcgcg aaaagcagtt tgccaaagtg gatctcaacg ccgacaagtc cctgagcttg
      601 gatgagatca agtccatgaa acaagtcggc ggcgacgaaa cgaaggccaa ggcattcttg
      661 gagcgactcg atgatgacaa ggatgggaag atttcagtaa aggaattcac gcgggtccgt
      721 gctatcttcc aattgccgcc gcaggaaaaa gagaaaaagg aaaagaaggg cgcaaactga
      781 tagttggcgg taagtcgcga ttaccaagct aatcttacct tctgagaatc gtttcgatta
      841 gtgagaaaag ccccgttggg cggtgagccg gcggggtttt cgttttctca tgtatgccaa
      901 accggcctgt tatagcaggt tcctcgtcag gcggggctta tcagtttgtt ctgtccagac
      961 cggccataac gcagggttat tgcgatgcca ttatggcgtc atcctcaagg gatgaaagga
     1021 ttcgtcacgc gcatcggtag gaccgcggaa gacgttgtga tgcgaaccgt gggtttgggc
     1081 tccaacagtg gcggggctat caccctgtcc attgtgcctt ctgtcttttc gccgcagaaa
     1141 agattgagca ccactaccgg atgtggccgc tcgcccggtg ggcactcagt cctacttccg
     1201 gcaccggcag gatgaagttg cggcttagga acgatcagac cgtttcgaag gcccgccaaa
     1261 taagttgcat agctaagtcg agcccgccag tcccattggt caccatatcg ggcaaaatcg
     1321 gagcggtaaa gcatgcttgc ccaaaactgg gcagaggatc ggtcgatttc gacgatagga
     1381 aactgggcaa aatagggtac catgtgtgtt tcgaagacgc ggtgacaata agcccccggc
     1441 agtcgcattt ccaccttgca cacctctgag tagggaa
//
LOCUS       DAOPTR010001047         1476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_67479_length_1476_cov_3.616467, whole genome shotgun sequence.
ACCESSION   DAOPTR010001047 DAOPTR010000000
VERSION     DAOPTR010001047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67479_length_1476_cov_3.616467"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PKI05_15630"
     CDS             <1..709
                     /locus_tag="PKI05_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53683.1"
                     /translation="LIALQKVGRGEVVYIGTNEMWRLRRKFGEKYYRQFWGQMIHRLA
                     IQHALGAEKRFVVRSDHREYRTGDEAVITVEAYDADFHPLDAAQLSESWLVGTLFVPT
                     ESQHQVREIKLTQTRPGWFETRIPLYNAGEYRLVVKDPLANQEVETRFVVSHQSVEFQ
                     QPTRNVERQRLLAQLTGGKSYELSEWTNLLTDLPQEPRTETVIRELEIWNTWPFFIFV
                     VGLLLCEWFLRKWLNLR"
     gene            691..>1476
                     /locus_tag="PKI05_15635"
     CDS             691..>1476
                     /locus_tag="PKI05_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53684.1"
                     /translation="MVESAVRSELAPLHRLLGRIGRKRQRLRLTIGILGVLVGLIFVA
                     LGLFTLDWLLGLTIAQRVVLILVAIIVAYVASRRFILPWFIGRESELDLALLVQKHFG
                     IDSDLVAALQFEYPEAVEWGSVELEQKVIRQVAAQAQQLPLIEDVPRAPLRKRLLASL
                     CGVTLVTALVLLFPEHVAVFVKRLMLQNVHYPTATRIIDLSVNGQPVSWDKAGQTLRC
                     GVGATVDIRVVATGRLPEQGQALFQIVDQRRNLRIPLVPVENQP"
BASE COUNT          317 a          373 c          415 g          371 t
ORIGIN      
        1 gctcatcgcc cttcagaagg ttggacgggg tgaagttgtc tatatcggta ccaacgaaat
       61 gtggagactg cgtcgaaagt tcggggaaaa gtactaccga cagttttggg ggcaaatgat
      121 ccatcggctg gctattcaac atgccctagg ggccgagaag cgattcgttg ttcgttctga
      181 tcatcgtgaa taccgcacgg gcgatgaggc cgtcattacc gttgaggctt acgacgccga
      241 ttttcacccc ttggatgccg cgcaactttc cgaatcgtgg ttggttggaa cgctctttgt
      301 tcccacagag tcgcagcacc aggtgcgcga gattaaatta actcaaacgc ggccggggtg
      361 gtttgaaact cgtatcccgt tgtacaacgc cggtgaatac cgtctagttg tgaaggaccc
      421 tttggcaaac caagaggtgg aaacacgttt tgtcgtgtcg catcagtccg tcgagtttca
      481 acagccgacc cgaaatgtcg aacgacagcg cctgctggct caactgaccg gggggaaaag
      541 ttacgaactg tccgagtgga cgaatctttt gacagatttg ccccaagagc cgcgaactga
      601 aacggtgatt cgtgaattag aaatctggaa tacctggcca ttcttcatat tcgtcgtggg
      661 attgttactt tgcgagtggt ttctgcgcaa atggttgaat ctgcggtaag aagcgaactg
      721 gcgccgctcc accgactttt aggccgaatt ggccgcaagc gacaacggct tcggctgacc
      781 atcggcatcc tcggggtgct agttggtctc atttttgtcg ctttggggct ctttacactg
      841 gattggctct tgggcctgac aattgcccag cgagtcgttc tgattcttgt tgctatcata
      901 gtcgcctatg tggcatcgcg gcggtttatt ttgccatggt ttatcggtcg cgagtccgaa
      961 ttggacctcg ccttgctggt tcaaaaacac ttcggcatcg acagtgacct ggtggcagcg
     1021 ctgcagtttg agtatccaga agccgtcgaa tggggctccg tcgagcttga gcaaaaggtc
     1081 atccggcaag tggcagccca ggcacagcaa ctccctttga ttgaggacgt gccgcgggca
     1141 cctttgcgaa aacggctgct ggcaagtctc tgtggcgtga ccttagtaac cgcccttgtt
     1201 ttgctattcc ccgaacatgt tgcggtcttt gttaaacggc tgatgttgca aaacgttcat
     1261 tatccgacgg caacaaggat cattgacctg tccgtcaatg ggcagcccgt tagctgggat
     1321 aaagctggtc aaaccctccg gtgcggtgtg ggcgcaacgg tcgatattcg cgtcgttgcc
     1381 acgggaagac tcccggaaca aggtcaggcc ctttttcaaa tcgttgatca acgccgtaac
     1441 ctgaggattc ctctcgtacc ggtagagaat caacct
//
LOCUS       DAOPTR010001048         1474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_67632_length_1474_cov_2.439042, whole genome shotgun sequence.
ACCESSION   DAOPTR010001048 DAOPTR010000000
VERSION     DAOPTR010001048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67632_length_1474_cov_2.439042"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="PKI05_15640"
     CDS             <1..627
                     /locus_tag="PKI05_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HOA53685.1"
                     /translation="AIITKHSPEKGSCVQQCTYDLFEGENIEERKKRFHMAQWINPDR
                     GEMFFAQRVVFVEGETEKVVLPFLAEKLGVFNSDVSFIDCGSKHNLPLYIAIANAFKI
                     PYLVVHDEDPLPDPIPSTWNKDKIQEKQRTFALNQEIIRLVNTPLGRVEMLSPDFEGV
                     SGVSKNQGDKKGKALAALDYFETGDMSNIPERLKDIIYVAFAVRGIES"
     gene            624..>1474
                     /locus_tag="PKI05_15645"
     CDS             624..>1474
                     /locus_tag="PKI05_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53686.1"
                     /translation="MKDTLFIRLLHHNDKGIVLSEQIAALREGRLGGELYAVVPDSFR
                     QVPNTPFCYWVSERIRRLFAELPPFESEGRTVKQGLATADDFRFVRAWWEVPAGRILD
                     GGRYVEKKRRKTQGCEKTEKWKEERIREFQAWCRRRTWEGKRWAPFAKGGEYSPYYAD
                     IHLVVNWENEGMEIRNFVDLKTGRLNSRPQNIGYYFRPGLTWTYRTNAGFSVRVKPSN
                     TIHGHVGHSAFCKKNVLSFLSWFNSSVVAKLLLRMMTTYKWEVGYIQNLPVSPFSKIS
                     IDNLQSS"
BASE COUNT          399 a          305 c          372 g          398 t
ORIGIN      
        1 gccataatca ccaaacattc tcccgaaaaa ggcagttgtg ttcagcaatg tacttatgat
       61 ttgttcgaag gtgaaaatat tgaagaaaga aaaaaacggt ttcatatggc ccagtggatc
      121 aaccctgatc gcggtgagat gttctttgct caacgagttg tgttcgtcga gggtgagact
      181 gagaaagtgg tgctgccgtt tttagctgag aagcttggtg tattcaactc ggatgtttct
      241 ttcatcgact gcggttcaaa gcacaatctc ccattgtata ttgctattgc caatgcgttc
      301 aaaattcctt atctggttgt acatgatgaa gatcccttac ccgatcccat accgtccact
      361 tggaataaag acaagataca ggaaaaacaa cgaacctttg ccctaaatca ggaaatcatc
      421 cgtctggtga atacgccttt gggccgagtt gaaatgctat cgcccgattt cgagggtgtc
      481 agcggtgtat ccaaaaatca gggcgataaa aaaggtaagg cactggccgc attggactat
      541 ttcgaaaccg gcgacatgtc caatattcct gagcggctta aggatattat ctatgtggcg
      601 ttcgctgtta gaggaattga atcatgaaag acactctttt catccgtcta ttacatcata
      661 atgataaagg tatcgtactg tccgaacaaa ttgccgcgct acgagaggga cgtttaggcg
      721 gtgaactata cgcggtcgta cccgattcat ttcgccaggt gcctaatacg cccttttgct
      781 actgggtgag cgaacgtatc cgtcgacttt tcgccgaatt gccccccttc gagagcgaag
      841 gacgtacggt taagcaaggg ttggcgaccg ccgacgattt ccgtttcgtg cgcgcttggt
      901 gggaggtccc tgccggtaga attttggacg gagggcggta tgtagaaaaa aagagacgaa
      961 agacacaagg ctgtgagaaa acagagaaat ggaaggaaga gaggatacgg gagtttcagg
     1021 catggtgccg gcggcgtact tgggaaggga agcgctgggc tcctttcgcc aagggcggtg
     1081 aatattctcc ttattacgct gacattcatc tggttgtgaa ttgggaaaac gaagggatgg
     1141 aaatacgcaa ttttgttgat cttaaaaccg gaaggctgaa ttcgagacct cagaacatcg
     1201 gttattattt tcgtccggga ctgacgtgga cttaccgcac taatgccggt ttttctgtgc
     1261 gtgtaaagcc ttccaacaca atacatggac atgtcggcca cagtgcattt tgcaagaaaa
     1321 atgttttgtc attcttgagt tggttcaatt catcagttgt cgcaaagtta ttacttcgga
     1381 tgatgactac ttataagtgg gaagttggtt atatacagaa cttgccagtt tcaccttttt
     1441 cgaaaatcag cattgataat ctacaatctt cgca
//
LOCUS       DAOPTR010001049         1469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_67963_length_1469_cov_2.830269, whole genome shotgun sequence.
ACCESSION   DAOPTR010001049 DAOPTR010000000
VERSION     DAOPTR010001049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67963_length_1469_cov_2.830269"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1469)
                     /locus_tag="PKI05_15650"
     CDS             complement(<1..>1469)
                     /locus_tag="PKI05_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53687.1"
                     /translation="GRNLRWIGPNGQTLTETQAPYRGMAPYYNILHREVQEELLAVVR
                     ELVERYGRHASFYGLGIQISAYGYAQLPGPEWGLDDETIARFSEETGIQLQVPDDNRY
                     QERARLLLGPYSREWLRWRSLRLQTFYSRVTQEVAAVRPDAKVLLLAPTMFVGERWEQ
                     RLRPTLTERRDPLALLLETGLTPELATSDLAMGLLRVKRMVEHADFGVRTTEFEIDQI
                     YRSIPMNAQGHMPGVLFYHPPQEIRLPSFDARSPIQPAFTAIIHQPVAWGLENRRRYA
                     QALADGDPTLICDGGWVTPWGGEDMLRPWLAAFRRLPGGPFQDVRFPDQGTSLQPLVV
                     RSLTRQQETYLYFANQVGFPVILEMTLQAPRGCRMIELSGFRSLPPLESDSQGQCRWM
                     MTFEPYDLVAVKLTTADITFTSAKVSWSPKVEQRLREQIADLSERAATLSVPPLYAVL
                     ENPDFEEPAGDAQPVPLWTALAPAGTMVRVDAAERHGGQ"
BASE COUNT          302 a          427 c          452 g          288 t
ORIGIN      
        1 ctgcccaccg tgtctttccg ccgcatcgac tcggaccatt gtccccgcgg gtgcgagggc
       61 cgtccacaac ggcaccggtt gcgcgtcacc ggccggctct tcaaaatcgg gattttcaag
      121 gacggcataa aggggaggga cgctgagcgt ggcagcacgt tcactcaaat cggcgatctg
      181 ttcccgcagt cgttgttcga ccttaggtga ccaactcacc ttggccgatg taaacgtgat
      241 gtcggcagtc gtcagtttga cggccacgag atcgtacggc tcaaaggtca tcatccagcg
      301 gcactgtccc tgggagtcgc tttccagagg cggtaatgag cggaagccac tcagttcgat
      361 catgcggcag ccccgcgggg cctggagcgt catctccaga atcacgggga agccgacctg
      421 attggcgaag tagaggtagg tctcctgctg gcgtgtcaaa ctgcggacca cgagcggttg
      481 gagtgaggtc ccttgatcgg ggaaacggac atcttggaat ggtccccccg gcaaacggcg
      541 gaaggcggca agccacggcc gcagcatatc ctcgccaccc caaggggtca cccagccgcc
      601 gtcacagatc aatgtggggt ctccatcggc cagggcttgg gcgtaccgtc gccggttttc
      661 tagcccccag gccaccggct gatgaatgat ggccgtgaag gccggctgaa tgggtgatcg
      721 ggcatcgaag ctaggcaaac gaatttcctg cggcggatga tagaacaaca cgccgggcat
      781 gtgtccttgg gcgttcatgg ggatcgaccg gtagatttgg tcaatctcaa actccgtggt
      841 gcgaacgccg aaatccgcat gctcaaccat ccgtttgacg cggaggaggc ccatggccaa
      901 gtcgcttgtg gccagctcgg gcgtcaatcc cgtttctagc aggagggcca gcgggtcacg
      961 acgttccgtc aacgttggac ggagacgctg ttcccagcgt tcgcccacga acatggttgg
     1021 agccaaaagg aggacctttg catcaggccg aacggctgcc acctcttgtg tcacgcgaga
     1081 ataaaaggtt tgaagtcgca gcgagcgcca cctcagccat tcgcgggaat agggtcccaa
     1141 aagcaagcgg gcccgttctt gataacggtt atcatcgggc acctgaagtt ggatgcctgt
     1201 ctcttcagaa aatcgggcga tggtctcgtc atccaatccc cattctggtc cgggaagttg
     1261 tgcgtagccg taggccgaga tctgaatccc caatccataa aatgaggcat gacgcccata
     1321 acgttcaaca agttcacgaa cgacggccag aagctcttcc tggacctcgc ggtggaggat
     1381 gttgtagtag ggcgccatcc ctcgataagg cgcctgtgtc tccgtgagcg tttggccgtt
     1441 gggaccgatc cagcggaggt ttcgccccc
//
LOCUS       DAOPTR010001050         1468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_68024_length_1468_cov_3.142251, whole genome shotgun sequence.
ACCESSION   DAOPTR010001050 DAOPTR010000000
VERSION     DAOPTR010001050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68024_length_1468_cov_3.142251"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="PKI05_15655"
     CDS             <1..696
                     /locus_tag="PKI05_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008695537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOA53688.1"
                     /translation="VEDEVKGIKRTRLQFAKTKAPLIRVDRATMPDFANYYEAHLMNP
                     KVLVGVLIGAMLPFLFSSLTMKAVGRAAYRMVEEVRRQFREKPGIMEGTEKPDYATPV
                     RISTQAAQKEMILPSLLGLLTPVAVALILGVTGVMGLLMGALASGFCIAVFMANAGGA
                     WDNAKKHIERGAYGGKGTDAHKAAVVGDTVGDPFKDTSGPSLNILIKLMSMVSVVVAG
                     LAVRYSLASLGWF"
     gene            complement(813..1376)
                     /locus_tag="PKI05_15660"
     CDS             complement(813..1376)
                     /locus_tag="PKI05_15660"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOA53689.1"
                     /translation="MNLTPRFDEALQFAVSLHRDQRRKVNDAPYIGHLLRVCGLVLEY
                     GGTEDEAIAALLHDSLEDQNRPGLAEEIAARFGETVLSIVQACSDTVVQPKPPWRERK
                     EAFLERLRTANAAVRLIQAADKLDNVSILLGQYRVFGPEIWNKFKGGREGTLWYYRRV
                     AEVLGWASENPLVLELARRVEELEQLA"
BASE COUNT          300 a          428 c          428 g          312 t
ORIGIN      
        1 gtcgaggatg aagtcaaagg catcaagcgg acccgtctcc aatttgccaa gacgaaggcc
       61 ccattgattc gggtggatcg ggcaacgatg cctgacttcg ccaactacta cgaagcccac
      121 ctcatgaatc ccaaggtact ggtgggtgtc ctcattgggg ccatgttgcc gttcctcttc
      181 tcctcgttga cgatgaaggc cgtcggtcgt gcggcctacc gcatggtcga ggaggtccgc
      241 cggcaattcc gcgaaaagcc gggcattatg gaagggaccg aaaagcctga ctacgctacg
      301 cctgtgcgaa tcagcacgca ggccgctcaa aaggagatga tcctgccctc cttactgggg
      361 ctgctcacgc ctgtggccgt ggccttgatc cttggtgtga caggggtgat gggccttctg
      421 atgggcgccc tggcctccgg cttctgcatc gcggtgttca tggccaatgc cggtggtgcc
      481 tgggataacg ccaaaaagca cattgaacgt ggcgcttacg gcggaaaagg caccgacgct
      541 cataaagcgg ccgtcgtggg cgacaccgtg ggcgacccgt tcaaggatac cagcggtccc
      601 agcctcaaca ttctcattaa gctcatgagc atggtgagcg tggtggtggc agggctggcc
      661 gtccgttaca gtcttgcgtc gctcggttgg ttctgatacg ggccccaaga cctcgatgtg
      721 taaacatcga gccaggtagg tcatttcgac gaggcgggat ttacgtcccg cctctttttt
      781 tctcgctttt tgtcgctacg caaacgccgg catcaggcga gttgctccag ctcttcaaca
      841 cgcctggcga gttctaggac aagaggattc tcgctggccc aaccaagaac ctcagccaca
      901 cggcgataat accagagcgt cccctcgcga ccgcccttga acttgttcca aatctccggc
      961 ccaaagacgc ggtattgccc aagcaagatg ctgacgttgt ctagcttatc cgcggcttgg
     1021 atgaggcgga cggccgcgtt agcggtgcga aggcgttcca aaaaggcctc ctttcgttcc
     1081 cgccagggtg gcttgggctg aaccaccgta tcgctgcacg cttgaacaat ggacagcaca
     1141 gtctcgccaa agcgggcagc gatttcctcg gcgagtcccg gtcgattttg gtcctctagg
     1201 gaatcgtgaa gcagagcggc aatggcctcg tcttcggtgc ccccgtattc caaaacgagc
     1261 ccacataccc gcaggaggtg tcctatgtac ggcgcatcgt tgacttttcg tcgctggtca
     1321 cgatgcaacg agacggcaaa ttgcagggcc tcatcaaaac ggggggtgag attcatcgct
     1381 ctttatcacg aaaatgggat gacttacggt gcgccgcctt cgtaaacact aaattcggcc
     1441 caaaccggca ggtgctgggc aacctgtt
//
LOCUS       DAOPTR010001051         1467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_68097_length_1467_cov_2.439093, whole genome shotgun sequence.
ACCESSION   DAOPTR010001051 DAOPTR010000000
VERSION     DAOPTR010001051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68097_length_1467_cov_2.439093"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(923..1300)
                     /locus_tag="PKI05_15665"
     CDS             complement(923..1300)
                     /locus_tag="PKI05_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53690.1"
                     /translation="MDRYSPVNWVTFMLSLALVAVLANPCQRAFAQTQAKSSSSKSSL
                     HLPHLPNPITALENGAKKVASAVGSLIPGKQQSTSSTRYYPPEGSKKKTEKKGLFSWL
                     APKPEPKKSDSVCDWIGREPLLP"
BASE COUNT          345 a          393 c          418 g          311 t
ORIGIN      
        1 tcaaacgact ccgtcgtggc acgcataatc ttccaagccc ccggttcgaa gcgtccccaa
       61 gggtgcacct ggcggggaac atcgacagcc agcgtaatgc cactccatcc agtgaaaatg
      121 cagacgagta atgggaccaa ccagggccac ccggcccgct gtcgattcca ccgcccatcg
      181 cgtctacata aatgagcgta atcggaattc gatccacgac ccgcaataaa aacttgcttg
      241 gcaacgggct gatcagatga aagagccatg acgggaatgt tagctttgtg gcgagacctt
      301 attcgattca cgaggacgaa gcgaccgtcc tttctggtga gaaagaccga tgtggtattc
      361 cacgacccgt ccgtgccaga ccccccaagc ctttgtcgtt gttatacccc ccgaaaagtg
      421 acattgcaag cggccggcgg cacgcttacc aagagcctat cccacaagct cccctcgccc
      481 gctcggcggg agaggggttg ggggtgaggg ttgcgcgaat cgaggtcggt tttctcgatc
      541 gactccgaca acggcttgcc ctcaccccaa ccctctccca gagggagagg gggacttttt
      601 aggaaagact ctaagcaccg tgctgtgggc cactttcact tgcgttgcaa cctgatcagc
      661 gaatgccggc ttgttgggcc gtgggaaagg ggtcaccggc cacccgttgc tgaagcgatc
      721 ggtcggtttg agctgaggcc gataggagtg tggcgcaccc ggtaagacac cccgttgcga
      781 ggactagcag aagaatcgaa gcaaccagtt gggggaagtg cttgaactgc attgaacatc
      841 ccaaaacaat ggccacgttt tgcgggtctc aggcgaatac cataaaaagc tcttctatga
      901 ggtactcaat caaaaacagc gattacggca acaagggttc tctaccgatc caatcgcaaa
      961 cggaatcaga ttttttgggt tcgggttttg gggccagcca gctaaagagg ccttttttct
     1021 ccgttttttt ctttgaacct tcaggcggat agtaacgggt cgaggaagtg gattgctgtt
     1081 tgccggggat gagagagcca acggcgctcg ctactttttt cgcgccattt tcgagggctg
     1141 taatggggtt gggcaggtgc ggtaggtgca gggaactctt agatgaggaa ctcttcgctt
     1201 gggtttgtgc aaaggcacgc tggcacggat ttgccaacac cgccaccaac gcgagagaca
     1261 gcatgaatgt cacccaattc actggcgaat accggtccat ggcctagcct ccacgttgac
     1321 attgcgtcct accgtcgtat caacggaacg gattgtaaca gttaccaaaa acgccgtgtg
     1381 tttgtccagg ccaagctgat tggccgtggg cggtagaagg cggatttgcc agggattccg
     1441 cagattagac gggagcaacc aaaatca
//
LOCUS       DAOPTR010001052         1467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_68108_length_1467_cov_2.109065, whole genome shotgun sequence.
ACCESSION   DAOPTR010001052 DAOPTR010000000
VERSION     DAOPTR010001052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68108_length_1467_cov_2.109065"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..>1467)
                     /locus_tag="PKI05_15670"
     CDS             complement(168..>1467)
                     /locus_tag="PKI05_15670"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HOA53691.1"
                     /translation="SEDGYVVVKKLIPGGAAAKDGRLKIDDKIVGVGQGETGEIVDVV
                     DMNLSDVVKLIRGKRGTVVRLEVLPADGGPKKLITITRERIELKDSEAKGQVFEAGQK
                     PNGQPYRIGVIDLPSFYMDMSGARLGIPEFRSTTRDVRRILESFNQQGVDAVVLDLRR
                     NGGGSLQEAINLTGLFIESGPVVQVKDSVGRIMVYDDEDTSIVWRGPLIVVISKFSAS
                     ASEILAGAIQDHRRGIIVGDQSTHGKGTVQSLIDLGQELFRLPNAPPLGALKLTIQQF
                     YRPNGESTQNRGVIADIEIPSLTNHLEGIAESDLDYALPFDQIRPAQYKPYSDVDSSV
                     IEYLKRRSEERVQNSPDFQKVKADIQRYLAQREKKSVPLMEEKFMAQVKELNADKEEE
                     KRLKALTEGNEEGIERDYYLDEVLQIMVDYLQYRVIAQAR"
BASE COUNT          326 a          439 c          347 g          355 t
ORIGIN      
        1 tgtcctcttt gaactgcgcc caggcaaggg tctgtaccgc caacatcaga aggcaaaccg
       61 tactcaggca aacgatgcgt cgcattgttt ccagacaatc caaatggctt ttccccaaag
      121 caacgtggcg gaccgctctc gtgaacgatg aaggtaagcc ggaaagtcta gcgggcttgg
      181 gcaattaccc gatattgcaa ataatccacc ataatctgga ggacttcatc gaggtaataa
      241 tcccgctcaa tcccctcttc attaccttcg gtaagggcct tgagccgctt ctcttcctct
      301 ttgtcggcat tcagttcctt cacctgggcc ataaactttt cttccatgag gggcacactc
      361 ttcttttccc gttgggcaag ataccgctga atgtcggcct tgaccttctg gaagtccggc
      421 gaattctgca cgcgctcctc ggagcgtcgt ttcaggtact cgatcaccga cgaatcgaca
      481 tcggaatagg gcttgtattg cgccgggcgg atttgatcaa acggcaaggc gtaatccaaa
      541 tcagactcgg caattccttc caaatggttt gtcaaagacg gaatctcgat gtcggcaata
      601 acgccccgat tttgtgtgct ctcgccgtta ggccgataga actgctgaat cgtaagtttc
      661 agcgcaccta gcggtggggc attgggaagc cggaacagct cttgtccgag atcaatgagg
      721 ctttgcaccg ttcccttgcc atgcgtgctt tggtcaccca cgataattcc ccgacggtgg
      781 tcttgaatgg ccccggcgag aatctcgctg gcactggcac tgaacttact gatgaccaca
      841 atcaagggac cccgccaaac aatgctggtg tcttcatcgt catagaccat gatccggccg
      901 acggaatctt tgacctgcac gaccggaccc gactcaatga ataatcccgt cagattaatg
      961 gcctcttgaa gcgacccacc accattccgc ctcaagtcca aaacaacggc gtccacacct
     1021 tgttgattaa aggactccag aatccgcctg acatctcgcg tcgtgctgcg aaattcaggg
     1081 attcctaacc gcgcccctga catatccatg taaaagctgg gaagatcaat cacgccgata
     1141 cggtagggct gtccattcgg tttttggccg gcttcgaaga cctgcccttt tgcctcgcta
     1201 tctttcagtt cgatcctttc ccgggtaatc gtgatcagct tctttggtcc gccatcagca
     1261 ggcaacactt ccaagcggac gacggttcct cgcttgccgc gaataagctt gaccacatcc
     1321 gaaagattca tatcgacgac atcgacgatt tcgcccgtct cgccctggcc gacgcccacg
     1381 atcttgtcgt cgatctttag ccgtccgtcc ttggctgcgg cgccaccggg aatcagtttc
     1441 ttgaccacca cgtaaccatc ttcggac
//
LOCUS       DAOPTR010001053         1465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_68216_length_1465_cov_3.556028, whole genome shotgun sequence.
ACCESSION   DAOPTR010001053 DAOPTR010000000
VERSION     DAOPTR010001053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68216_length_1465_cov_3.556028"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="PKI05_15675"
     CDS             complement(<1..801)
                     /locus_tag="PKI05_15675"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOA53692.1"
                     /translation="MKSTLLCAVVIFVVFRMLPIGLCGEIRFRHHFVDRDLPGNSWGQ
                     TAVADLDRDGRLDYITGKSRGQIVWYRQISLTQWERHILGEDSPSDVGGAVLDVDGDG
                     WMDFVTGGAWYRNPQRPTEGPFERIVFDKELQGVHDVVVADVNGDGRLDVLTMSDRNN
                     IRWYQIPENPRELWVRTDIGPSVHAGLAVGDLDGDGDLDVIRSNLWFENVDGKGKTWN
                     VHENIPFGNPSPPYALATRCIAVDIDSDGDLDLVMTENEIRGGRIGWLE"
     gene            1097..>1465
                     /locus_tag="PKI05_15680"
     CDS             1097..>1465
                     /locus_tag="PKI05_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53693.1"
                     /translation="MNGRHFFWTVLVATSLISQLALAQELYVGLSVVDITPSEPVALD
                     GQRHVRIAKEAATPITATVLAMESRQGEQVLDQAVIVSCDLVAIREGVCQMVREKVVQ
                     LKSDLDPLKIFLCATHTHTAP"
BASE COUNT          336 a          427 c          372 g          330 t
ORIGIN      
        1 ctccaaccaa ccgatccgtc ctccgcggat ttcattctcg gtcatgacca gatccagatc
       61 gccgtcgcta tcgatatcga ctgctataca tcgggttgcc aaagcgtacg gcgggctggg
      121 attgccgaag ggaatgtttt cgtgcacatt ccaggtcttt cctttgccgt ccacattttc
      181 gaaccagaga ttcgagcgga tgacgtcgag atcgccgtcc ccgtccaaat cgccgacggc
      241 caggcccgcg tggacggatg gcccaatgtc ggttcgcacc cacagttccc gcggattttc
      301 cggaatctgg taccaacgga tattgttccg gtcagacatg gtaagcacat cgagacggcc
      361 atcgccgttt acatccgcaa cgaccacgtc atgaacccct tggagttctt tgtcaaagac
      421 aatccgttcg aaaggccctt ccgtaggtct ctgcggattt cgataccacg ctccacctgt
      481 gacaaaatcc atccaaccat cgccatcgac atccaacacg gccccaccta catccgaggg
      541 cgaatcctca cccaatatgt gacgttccca ctgggtcaaa gagatttgcc ggtaccaaac
      601 gatctggcca cgacttttac cggtgatgta atccaaacgt ccgtcgcgat cgaggtcggc
      661 cacggccgtt tggccccacg aattgcctgg gagatctcga tccacaaagt ggtgccgaaa
      721 gcgtatctca ccgcacaatc ctattggcaa cattcggaag acgacgaaga tgacgaccgc
      781 gcataaaaga gttgatttca tcgccagact cctttttacc agagatgatt cttgggccac
      841 gataggctca tttgacaact tgactggccc atgtcaccca gcgacggaag cgctcatttg
      901 cggctatctt gtgataacac caatgaagcg aatcggcaag acctccggca agcgagatgc
      961 gtcggggccg gtcgctgcaa tgattgacag ccattaggtg cggggctagt atgatggcac
     1021 cacgataagg gagccacatc agagactcac agtcagtcgg ttagttgcac ttttttctgt
     1081 agataaggag ccagtcatga atggccgcca ctttttttgg acggtccttg ttgcgacttc
     1141 gttaatctcg caacttgctt tggcccagga gctttacgtt ggactgagtg tcgtggacat
     1201 cacgccatcc gagccggttg ctcttgatgg ccagcgacac gtgcgaatcg ccaaagaagc
     1261 ggccacaccc atcacggcca ctgtgctcgc catggaatcc agacaagggg aacaagtctt
     1321 ggatcaggcc gtgattgtgt cttgcgatct tgtcgccatt cgcgagggtg tttgccaaat
     1381 ggtgcgggag aaggtagtcc agctcaaaag cgatctcgat cccctcaaga tattcctttg
     1441 tgccacgcat acacataccg ccccg
//
LOCUS       DAOPTR010001054         1465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_68230_length_1465_cov_2.704965, whole genome shotgun sequence.
ACCESSION   DAOPTR010001054 DAOPTR010000000
VERSION     DAOPTR010001054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68230_length_1465_cov_2.704965"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PKI05_15685"
     CDS             <1..635
                     /locus_tag="PKI05_15685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOA53694.1"
                     /translation="NVLKWGTGALNIDGCRIPTDEVIMNHSRSAEAAKSKGKYGDSKA
                     QETQQTPGQMLGRFPANVLLDEEAARLLDEQTGPLHSQDPATRISRSKAKGVTGFGTG
                     VSVEYGDSGGASRFFYVAKASKRERTMNGKVENNHPTVKPIKLMRYLCRLITPPGGLI
                     LDPFAGSGTTLLAAIQEGFSRIGIEKEPEYCEIIRQRMAEVQPVLPLEES"
     gene            799..1356
                     /locus_tag="PKI05_15690"
     CDS             799..1356
                     /locus_tag="PKI05_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53695.1"
                     /translation="MIQDVIDDKVTRAKAILRTAQENPAWWCEKALGVTPTDFERFCL
                     DSWKAAKSLSFRQTIPLGERQRAAALMLMAFLHLWQDALVITLAPNERLLGAGLWPEV
                     ESLYRGQRINLGSIMGPYEVRCGPTWFARGFGGDDPALLQGFVPSGEHVLVLVENTAN
                     VDARSLELMRALTAISPHGYFLEVS"
BASE COUNT          328 a          374 c          455 g          308 t
ORIGIN      
        1 agaacgtgct caagtggggt acgggggcac tgaatatcga cggatgccga atcccaacgg
       61 atgaagtcat tatgaatcat agccgttctg cggaggcggc gaagagtaag gggaagtacg
      121 gtgacagtaa ggcgcaggag acgcaacaaa cgccggggca aatgttaggc cgcttccccg
      181 caaacgtgct tttggacgag gaagctgcgc gactgcttga tgagcaaacg ggtccacttc
      241 atagccaaga ccctgcgacg agaataagtc ggagcaaggc taagggcgtc actggttttg
      301 gtactggtgt gagcgttgaa tatggtgatt cgggtggcgc gtcccgcttc ttctatgttg
      361 ccaaagcctc caaacgcgaa cgcaccatga acggcaaggt agagaacaat catcccaccg
      421 tgaagccgat caagctgatg cgctacctgt gccgactgat tactcctccc ggtggtctaa
      481 ttctcgaccc gtttgccggt tccggcacaa ccctcctagc ggcgatccag gagggttttt
      541 cgcgcattgg gatcgagaag gaaccggagt actgcgagat tatccgtcag cggatggcgg
      601 aggttcaacc ggtcttgccg ttggaggagt cataggtggc aaagctgcgt gccaacacgg
      661 aacagcaagt gtcctccacc ctctcctgca cccgcgccgc gctcctgtgc ggccgcacga
      721 tcaccgtcac gagagtggcc cacgggcgcg catgtagggc caatttgtag accgtgggcg
      781 tacaaggagg cgtgatgcat gattcaagac gtaattgacg ataaagtaac gagagctaag
      841 gcgatattac gaactgcaca agagaacccg gcatggtggt gtgaaaaggc gctcggcgtg
      901 acgccaactg atttcgaacg tttctgcctg gatagttgga aggcggccaa atccctgtcc
      961 tttcggcaga caatcccgct cggagaaagg caaagggccg ccgctttgat gcttatggcg
     1021 ttcttgcacc tttggcaaga cgcattggta atcacgctag cccccaatga gagattgctg
     1081 ggggccggac tttggccgga ggtggaatct ctttaccgcg gccagaggat caacctcggt
     1141 tcgatcatgg ggccatacga agtaaggtgt gggccaacat ggtttgctag gggattcggc
     1201 ggggatgatc cggccctgct ccaaggattt gtcccatccg gtgagcatgt gttggtgctc
     1261 gtcgagaaca cagcaaacgt tgatgcgagg tcactcgaac tcatgcgggc cttgacggcg
     1321 atttctccgc atggctactt cctcgaagtg tcctgaagtg caaggaggct aagatttgaa
     1381 ccgcatttgt ctcatcggtc gccttgtggc cgataccgtg tagttcctcg ataggccgaa
     1441 ggatgagtcg tctttgggtc ggggc
//
LOCUS       DAOPTR010001055         1464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_68299_length_1464_cov_2.488999, whole genome shotgun sequence.
ACCESSION   DAOPTR010001055 DAOPTR010000000
VERSION     DAOPTR010001055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68299_length_1464_cov_2.488999"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="PKI05_15695"
     CDS             <1..952
                     /locus_tag="PKI05_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53696.1"
                     /translation="QWSRVPVCVDPESAEWLRLTGNEEIQVEMQNVTFEDITRAMGEE
                     VGLSLVPCKQSVVLVAADRIRDHVRYEFQMEEGESGTFPSSTTLEQVCDWFNEVSPGG
                     FDKPAQVAKEGDRLVIDGPFPVVFEMRRMLEHRAAMGSTNERLDAEDEATISLNFFEP
                     TRLTDIAVAINRTAANKVFFDWTSLARIDVTPDTEVSISATDEPLSGVLERLAELIQA
                     QVHAWDDGPFVVCAPNRLTSGVLRIYYVGDILEKGTTPGQLREQLIRECAPESWADGG
                     GTGMLIYDAPAQAFVVLNSAIVHREISSWLQKRREGQQGQ"
     gene            1044..>1464
                     /locus_tag="PKI05_15700"
     CDS             1044..>1464
                     /locus_tag="PKI05_15700"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOA53697.1"
                     /translation="MRRFSKRSLLAVVKRYRDAVRNEPAVHRTAAWWLAYYQRSVAEL
                     LLIIWAGMGLFVGFRQLGDGLSLWFNTSSPRSYAFIVEVNSADWPELTCLPGIGEILA
                     KRIVMTRQEMGSFSRSEDLRTVKGIGPKTVRKIAPFLR"
BASE COUNT          330 a          326 c          444 g          364 t
ORIGIN      
        1 gcaatggtcg cgtgtccctg tgtgtgtgga tcctgaatcg gctgagtggt tacgcctgac
       61 cggaaatgaa gagattcaag ttgagatgca gaacgtcacg ttcgaagata taacaagagc
      121 catgggagaa gaggtaggct tgtcacttgt gccatgtaaa cagtcggtgg ttcttgttgc
      181 tgccgatcga ataagggatc acgtccgtta cgaatttcag atggaggaag gtgaatcagg
      241 gactttccca tcgtcaacca cgttagagca agtctgtgat tggttcaatg aggtgagccc
      301 tggtggtttc gacaaaccgg cacaggttgc aaaagagggt gatcgcctgg taattgatgg
      361 gccttttccc gttgttttcg aaatgcgaag aatgctagag catcgggctg ctatgggcag
      421 caccaatgag cgtctggatg ccgaagacga ggcgacgatt agtcttaact tcttcgagcc
      481 aacaaggctg actgatatcg cagtagccat caatcggacc gcagcaaaca aggtgttctt
      541 cgattggacc tcgctggcca gaattgacgt aacacccgat acggaggttt ctatttcggc
      601 gacggatgag ccgctctcgg gtgtcctcga gcgacttgcc gagttgatcc aagctcaggt
      661 ccatgcgtgg gacgatgggc catttgttgt ttgcgcaccc aatcggctga cttcgggcgt
      721 tttacggatt tattatgtcg gtgatatcct tgagaaaggg acaactccgg gacagttgcg
      781 cgaacaactt ataagggagt gcgctccaga aagctgggcc gatggcgggg ggacggggat
      841 gctcatctat gatgcacctg cccaggcctt tgttgtgctt aacagtgcca tagtacatcg
      901 tgagatatcc tcctggttac aaaaacggcg agagggccaa caagggcaat gagcggagag
      961 tacttacacg tggtatctcc tgcctgcttg ctgaagccca agcataaatg gggtcggaga
     1021 gtatggacag gcgacgcaag tttttgagac gattttcaaa acgctctcta ttggcggtgg
     1081 taaagcgata tcgcgacgct gtgcggaacg agcctgccgt ccatcgtact gccgcgtggt
     1141 ggttggcgta ttatcagcga agtgtggctg aacttctcct catcatttgg gcggggatgg
     1201 gcttattcgt cggttttcgg cagttgggtg atggtctctc gttgtggttt aacacgtcgt
     1261 ctcctcgttc atacgccttc atagtggagg taaactctgc cgattggccc gagttgacct
     1321 gcctgccagg catcggggaa attttggcta aacggatcgt gatgacgcgc caggaaatgg
     1381 gcagtttttc ccggagtgag gatctgagga ctgttaaggg tatcggccct aagactgttc
     1441 gcaagatagc gccgtttcta agat
//
LOCUS       DAOPTR010001056         1460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_68566_length_1460_cov_3.308185, whole genome shotgun sequence.
ACCESSION   DAOPTR010001056 DAOPTR010000000
VERSION     DAOPTR010001056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68566_length_1460_cov_3.308185"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PKI05_15705"
     CDS             complement(<1..299)
                     /locus_tag="PKI05_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOA53698.1"
                     /translation="MSTIGQRMGITAARRAFTLVELLVVIAIIGILMAVMLPAIQASR
                     ETARRGQCGNNLRLIGQALTRYYGSYECFPAGVESEQGPIVNEPRGRHVGWICPL"
     gene            complement(332..1420)
                     /locus_tag="PKI05_15710"
     CDS             complement(332..1420)
                     /locus_tag="PKI05_15710"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOA53699.1"
                     /translation="MRWYPGTESRELRDGKKLAVFPSSPCQRHGVTLTECLAVLLCIG
                     ILIGLLLPAVHSARETARRAQCQMNLQQIGLALRNYETLHTVLPAGTVENSGPVSNIP
                     LGFHHSWTVAILPWLDPPAGAHVRYDQSVYSPRNLQFLVSLGRTPSVYTCPSGLSFAS
                     ACADYAGCHDDRDKPIDEDDHGVFFRNSAVRSVEIPDGTAHTIFVGEKPRYGPEWGWM
                     SGTRATLRTTAIPIPQMLELIERQRIDGGPPMESAQDLIRMIRPDVWLTNVPFGDDVE
                     AAFSQASKVPELPEDPKVVEELQKFGEIPEGRPIIGGFGSEHPGVTNFLFGDMSVRPV
                     WHQVDPKVLRGLGNRLDGEGIPESLQNN"
BASE COUNT          341 a          435 c          382 g          302 t
ORIGIN      
        1 aacaagggac agatccagcc gacgtgtcgt ccccgcggtt cattgacgat ggggccttgt
       61 tcgctttcca cgccggctgg aaagcactca tagctgccgt aatatcgcgt gagcgcttgg
      121 ccgatgagcc tcaaattgtt cccacactgt ccccggcgtg ctgtttcgcg cgaggcctga
      181 atggctggta acatgaccgc catcaagatg ccgatgatcg caatgaccac caagagttcc
      241 accagcgtga aggctcgccg cgcagccgtt attcccattc gttggccgat cgttgacata
      301 ggatttgtcc tttgccttaa gaactcatgg tttagttgtt ttgcaaggat tcaggtatcc
      361 cttcgccatc caaacgattg ccgaggccac gcaggacctt cggatcgacc tggtgccaca
      421 cgggacgcac cgacatatcg ccaaacagaa aattggtcac accaggatgt tcagagccaa
      481 atccgccaat gatgggccga ccttcaggaa tttccccaaa tttttgaagt tcttcaacca
      541 cctttgggtc ctcaggcaat tccggcacct tcgacgcttg ggaaaacgcc gcttcaacat
      601 catcgccaaa tggcacattt gttaaccaca catcgggtcg aatcatccgt atcaagtctt
      661 gagcactctc catcggcggg cccccgtcaa tgcgttgacg ctcgatgagt tcgagcatct
      721 gggggatggg gatggccgtc gtccgcaagg tcgcgcgggt tccactcatc cacccccatt
      781 ccgggccata ccggggtttt tcccccacga aaatcgtatg ggccgttcca tcgggaatct
      841 caacactccg caccgcactg ttgcgaaaaa agacaccatg gtcgtcttcg tcaatcggct
      901 tatcccgatc gtcatgacat cccgcataat cagcgcaagc agacgcaaag gacaaccccg
      961 aagggcaagt gtacacagat ggggtacgtc ctagcgaaac aagaaactgc aagttacgcg
     1021 gactgtaaac gctttgatca tatcgcacat gggcgcctgc cggagggtcc aaccacggta
     1081 agatggcgac tgtccaacta tgatgaaaac ccaggggtat gttcgaaacg gggccgctgt
     1141 tctctactgt cccggcaggg agcaccgtat gaagtgtctc gtagttccgc aaggccaggc
     1201 caatttgctg gagattcatc tggcattggg cacgccgggc cgtttcgcgg gcactatgaa
     1261 cggccggaag caacaggccg atcaaaatac cgatgcagag aaggacggca aggcactccg
     1321 tcaaggtcac gccatgccgc tggcatgggc tcgacggaaa aaccgccagc tttttgccgt
     1381 cccgcaattc acgcgactca gtcccaggat accacctcac ggggtaactc ctcatgatgc
     1441 gcaaaccgat tcaacgcggg
//
LOCUS       DAOPTR010001057         1460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_68581_length_1460_cov_2.663345, whole genome shotgun sequence.
ACCESSION   DAOPTR010001057 DAOPTR010000000
VERSION     DAOPTR010001057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68581_length_1460_cov_2.663345"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1460
                     /locus_tag="PKI05_15715"
     CDS             <1..>1460
                     /locus_tag="PKI05_15715"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOA53700.1"
                     /translation="KFAPGEGFQYSNPGIAMLTYALAAAMRNAPDNNIRSLLRDRVYR
                     PIGIADNEWSIGYGRTFEVDGLPLVAAWGGGSFTTRATARIGRLVLKEGMWEGKQILS
                     REAVRQVTQSAGLPGDCGMGWWTNAGERFPFLPRDAVWGAGAGDEVLLVIPSLDLIMV
                     RYGNDLGPQAPSQENIGRFLFQPLMKAVLSHSQVSGQTQPPYPPSPVIRELQWAPPEE
                     IIRLARGSDNWPITWGDDDQLYTAYGDGWGFEPRVPKKLSLGLAVVRGMPPTIEGANI
                     RSESVEQYGEGARGKKASGMLMVDGVLYMLVRNAGNAQLAFSRDRGRSWEWADWKFTV
                     SFGCPVFLNFGPNYAGARDDYVYIYSPDSDSAYTPADRMILARVGKDALLKQEAYEYF
                     VGLDPTGQPRWSRDIHQRGAVFVHAGRCGRSGITYNPALKRYIWYQVYPQSDHPEGPR
                     FSGGCGVIDAPEPWGPWTTVFHTDRWDVGPGESGCF"
BASE COUNT          300 a          380 c          462 g          318 t
ORIGIN      
        1 aaagtttgcc cccggcgagg gctttcaata cagtaacccc ggcatcgcga tgctgacata
       61 cgccttggcc gcggccatga gaaatgcgcc cgacaacaac atccgcagtt tgttacgcga
      121 ccgagtgtat cgacccattg ggattgccga caacgagtgg tcaattggtt acgggagaac
      181 attcgaggtg gatggcttgc cgcttgtggc ggcctggggt ggcggaagct tcaccacccg
      241 tgcgactgcc cgcattggac gcctggtact caaagaaggt atgtgggagg gcaagcagat
      301 tcttagccgg gaggcggtcc gtcaagtgac gcagagcgcg ggactgccgg gcgactgtgg
      361 aatgggttgg tggacgaatg ccggcgagcg gtttcctttc ttgccgcgag acgctgtctg
      421 gggagccggg gcaggggatg aggtcctgct tgttatcccc agcctcgatt tgattatggt
      481 tcgctatgga aatgatctag gtccccaggc accctcgcaa gagaacatcg gtcggttcct
      541 tttccagcca cttatgaagg cggtcctttc gcactcccaa gtctctggcc aaacgcaacc
      601 accgtatccg ccgagtcccg taatcaggga gttgcaatgg gcaccaccgg aagagatcat
      661 tcggctggca cgagggagtg acaactggcc catcacgtgg ggcgacgacg atcaactcta
      721 cactgcctat ggagatggtt gggggttcga accacgggtt cccaaaaaac tcagcctcgg
      781 attggccgtc gtccgcggaa tgccgcccac tattgagggt gcaaatatcc gctccgaatc
      841 ggtggaacag tacggagaag gggcgcgagg taaaaaggcc agcgggatgc tgatggtcga
      901 tggcgtcctc tacatgctgg tccgaaacgc ggggaatgcc cagttggcat tttcccgtga
      961 tcgtggccgg tcttgggaat gggcggattg gaaattcacc gttagcttcg gttgcccagt
     1021 tttcctgaat tttggtccta actacgccgg tgcgcgggac gattatgtgt acatttattc
     1081 accagacagc gacagcgctt ataccccggc cgatcggatg atcttggcac gggtaggaaa
     1141 agatgccctt ttaaagcaag aggcttacga gtattttgtc gggcttgatc ctacgggaca
     1201 gcctcggtgg agtcgcgata tacatcaacg tggtgcggtc tttgttcatg cggggcgatg
     1261 tggccgatcg ggcatcacgt acaatcccgc cctgaaacga tacatttggt atcaggtcta
     1321 tccgcaaagc gatcacccgg agggaccgcg tttttcgggg ggctgtggcg ttattgacgc
     1381 cccagaaccg tggggacctt ggacgactgt ctttcacaca gaccggtggg atgttgggcc
     1441 gggcgaatca gggtgtttcc
//
LOCUS       DAOPTR010001058         1458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_68694_length_1458_cov_3.899501, whole genome shotgun sequence.
ACCESSION   DAOPTR010001058 DAOPTR010000000
VERSION     DAOPTR010001058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68694_length_1458_cov_3.899501"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PKI05_15720"
     CDS             complement(<1..618)
                     /locus_tag="PKI05_15720"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOA53701.1"
                     /translation="MLDEIPFNGRRAYSYLQTICEIGPRPSGSAGMKAQQEMLTAHFQ
                     KLGAEVTLQHFTGIDPRNRAPVPMANFIARFFPDRTERILLCAHYDTLPYPLRDRFNP
                     KGRFVGANDGGSGVAVLMELAHYMDKVPEKLGVDIVFFDAEEFIFSEWDPYFQGSEFF
                     ARSYVESRQQRKWRYRAAVLIDMVGDADLQIYQERNSAWWKDSRWI"
     gene            complement(967..1161)
                     /gene="rpsR"
                     /locus_tag="PKI05_15725"
     CDS             complement(967..1161)
                     /gene="rpsR"
                     /locus_tag="PKI05_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOA53702.1"
                     /translation="MVDGQYPRPLYVDYKDVELLKKLTNRQGKIVGRRKTGCSAVSQH
                     AITKAIKRARIMALLPFVGE"
BASE COUNT          339 a          434 c          363 g          322 t
ORIGIN      
        1 aatccaccga gagtctttcc accacgcgct atttcgctcc tggtagattt gtaggtcagc
       61 atccccgacc atatcgatta ggaccgctgc ccggtaacgc catttccgtt gctgacgcga
      121 ctctacgtac gatcgggcaa aaaactcaga tccctgaaaa tacgggtccc attccgaaaa
      181 aataaactcc tctgcatcga aaaagacgat gtcaacgccc agtttctcgg gcactttatc
      241 catgtaatgg gctagttcca tcaagacggc aacaccgctg cccccgtcgt tggcgccaac
      301 gaaccgaccc ttggggttaa atctgtcacg gaggggataa ggtaacgtgt cgtagtgggc
      361 gcacagaagg atccgctcgg tgcgatcggg aaaaaaacgg gcaataaaat tggccatggg
      421 cacgggggct cggttacgag ggtcaatgcc cgtaaaatgt tgcaaggtaa cttccgcccc
      481 cagcttctga aagtgggccg tcaacatttc ctgttgggcc ttcatccccg cggagccgct
      541 cggacgcggc ccaatctcgc aaatcgtttg cagataactg taagccctac ggccgttaaa
      601 agggatttca tctaacacca gccgagagcg ccctccacgg aatggaaatc cctgccacgc
      661 aacgaatcct gcgcaaccta ccaggataag acttgccaga aagaagccaa gctgccgcac
      721 agcggcgatc tttttggtcg ttgtattgga atcaccggcg tctgacccag caagtttcct
      781 ccgaggatcg ctgggatact gactaatagg aagaccttgt ttcggagctt ccgcggaagg
      841 gccgatcttt ctacgactgt gcctagacat tacgggcccg atcttggcta tgaacgtctc
      901 agcccggctg atgaacgccc gccatgccgt ccgcttgcgc ccaaaccgtg agaataagcg
      961 gtccgttcac tcgccgacaa acggcaggag ggccatgatg cgagcgcgtt tgatcgcctt
     1021 tgtgattgcg tgctgactta cggcggagca acccgttttg cgacgaccca caatcttgcc
     1081 ctggcggttt gtcaactttt tcagaagttc cacgtctttg taatcaacat acaggggacg
     1141 cggatactgg ccatcaacca tcaaagggtc acgtttccgc cgcttggtcc gaattcgctt
     1201 ttttgccatt tgttttcaga gatcctctga ttttccaaag ccaatcatcg acaataccca
     1261 accgcggcac cgcgatatat cgcctggtgc cctcataaag atcaggttac caaaattccc
     1321 gccagagaca agagagcccc accgccccac ctctgtagga gggcgaatca gcggcggaag
     1381 agccattctg ccaatcacaa tacgtcccgc ctgccctctt tttctttggc ccctccattt
     1441 tgtggcctga tggccgcc
//
LOCUS       DAOPTR010001059         1446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_69521_length_1446_cov_2.297628, whole genome shotgun sequence.
ACCESSION   DAOPTR010001059 DAOPTR010000000
VERSION     DAOPTR010001059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69521_length_1446_cov_2.297628"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="PKI05_15730"
     CDS             complement(<1..739)
                     /locus_tag="PKI05_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOA53703.1"
                     /translation="MRPRIVLCFPNEERHRRKIAETAPWADVVDVPQERIAEELLNAD
                     IFCGHAKVPVDWDAVVRRGRLRWIQSSAAGMDHCLVPSVIESEIIVTSASGLLADQVA
                     EHTVALVTAWLRSLPVFLRAQEKQEFIRRPTRDLHRSTILIVGFGGVGRRLAQILHTF
                     KTTLWAVDLFPVDCPPYVKQLVPSENLLSLLPGADIVILSLPLNDQTRGMFNRQLFAQ
                     MKRGSLLVNVARGPIVVTDHLIEAIQSG"
     gene            1159..>1446
                     /locus_tag="PKI05_15735"
     CDS             1159..>1446
                     /locus_tag="PKI05_15735"
                     /inference="COORDINATES: protein motif:HMM:NF012310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53704.1"
                     /translation="MRRREFLITSSVVGTNVVLGSQLVKSMENLVTVEGGQRMAFTQP
                     PLPYPMDALRPFLSEEQVTFHYGKHHAAYFRNLNNLVEGKPEAQLSLRELVI"
BASE COUNT          362 a          369 c          406 g          309 t
ORIGIN      
        1 gtccgctttg gatcgcctca atcagatgat cagtcacgac aatggggccc cgggccacgt
       61 ttacaagcag actaccccgt ttcatctgcg cgaaaagctg acgattgaac ataccgcggg
      121 tttggtcatt gagcggtagt gaaaggatga caatgtcggc gccgggcagc aaggaaagaa
      181 ggttttcgga aggaacaagc tgttttacgt aggggggaca atccacggga aaaagatcaa
      241 cggcccagag tgtcgtttta aaggtgtgaa gaatctgagc caatcgcctg cccacgccgc
      301 caaagccgac aataagaatg gtgctgcgat gcaagtctcg ggtaggcctg cgaataaact
      361 cctgtttttc ctgtgcgcgg agaaacacgg gcaaacttcg caaccaagct gtaacgagcg
      421 ccacggtgtg ctccgccacc tggtcggcaa ggagcccgga ggcgctggtg acgatgatct
      481 ctgattcgat cacggagggg accaggcaat gatccatccc cgctgccgaa gactgaatcc
      541 agcgtagtcg cccacggcga acgactgcgt cccaatcgac cgggacttta gcatggccac
      601 aaaagatgtc ggcattaagc aattcctcgg cgatgcgttc ctgaggcaca tcaacgacgt
      661 cggcccaggg cgccgtctcg gcgatcttac ggcgatgtct ctcttcgttg ggaaagcaca
      721 agacaatccg tggacgcatg aagcaaaatc tcccaagcgg taagtgtctt cattggagcc
      781 ccagatagga ttaccggcca atatttcgaa gactccacag cgccaccaca gtcatgctgc
      841 cccaaccaac gttagtgcac gacagctctg agtgttcgcg gcggcttgtt atgaagtcgt
      901 tgttgctcaa ccggtaagat aggcctaatc tcgtctcctg gggagggggt gttcgggacg
      961 gcctaaaacg gcgatctagc tttcaattca attgcttgca tggcgtaaac caaggtgtta
     1021 tattgaccgc caaaaagata cgcggtgcct gcaatgtcga tggtgcgccc ttatcttcga
     1081 aaagtggcga tgtatggtac ggaaagggag ggtttgagaa actcgtcttg aatgcgcctt
     1141 tcacatggga gggaaatcat gagacgtcgc gagtttttga tcacttcatc ggtggttggt
     1201 acaaacgtgg tccttgggtc gcaactggtc aaaagtatgg aaaatctggt cacagtagaa
     1261 ggaggacaaa ggatggcatt cacgcaaccc ccgttgcctt atccaatgga tgctcttaga
     1321 ccgtttttgt ccgaagagca agtcacgttt cactatggca agcaccacgc cgcgtacttc
     1381 aggaacctca acaacctggt ggaagggaaa cctgaagccc agttgtcact tcgcgaattg
     1441 gtcatc
//
LOCUS       DAOPTR010001060         1441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_69849_length_1441_cov_2.820346, whole genome shotgun sequence.
ACCESSION   DAOPTR010001060 DAOPTR010000000
VERSION     DAOPTR010001060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69849_length_1441_cov_2.820346"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1441)
                     /locus_tag="PKI05_15740"
     CDS             complement(<1..>1441)
                     /locus_tag="PKI05_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53705.1"
                     /translation="PHPLLGDAQEWLVGSGVPDLADKIEQKWLALANTLRSALAKASD
                     LQTLVIDYPPNEAIFPPEIIPPTILWHDSAEPADTWLVEFTVGDGQAWVGALVPGSPP
                     PQGEIDPRCIAETNEIYRGTPYQQSAKSWTVHDNIWQWVKQQSVERPAQLRIVGFSSR
                     RPHVPLSVGQVTLSTSRDPVGAPIFYRDVPLAPSATERGVIKPLSEAFLPLIAWRLRD
                     ISQPKSRLLLTNLLTCANCHSFSDDGTTLGMDVDGPSGDKGAYAIVPVARETIIDTKD
                     VISWNSFSGKPPGHKTIGFLSRVSPDGRYVVTTLNESVYVQNFKDYRFLQVFYPTRGI
                     LGYYDRENGEIKPLPGADDPAFVHCDPVWTPDGQFIVFARAEARDPYPPGYRPAQRAN
                     DPAEPQIQYSLYRIPFNGGRGGTPEPIEGASHNGMSNTFPKVSPDGKWIVFVKCRNGQ
                     LLRPDSELWIVPATGGTARKMRCNTPLMNS"
BASE COUNT          337 a          396 c          410 g          298 t
ORIGIN      
        1 acgaattcat gagaggcgta ttgcatcgca ttttgcgggc cgttccgccg gtggcaggaa
       61 caatccaaag ctcgctatca ggccgaagga gttgcccatt gcggcatttc acgaacacga
      121 tccattttcc atcgggggag accttgggga atgtgttgct catcccgttg tgcgacgccc
      181 cttcaatcgg ttctggtgtg cccccgcggc ctccgttaaa cggaatccga tagaggctgt
      241 attggatttg cggttccgcc gggtcattcg cccgttgcgc aggtcggtat cccggcggat
      301 agggatcacg ggcctcggca cgggcaaaga caatgaactg cccatccggc gtccaaaccg
      361 gatcacaatg gacaaaggcc gggtcgtccg cacccggcag tggtttgatt tctccattct
      421 ctcggtcgta ataacccaaa atccctcgcg tgggataaaa cacctggaga aaacgataat
      481 ccttgaagtt ttggacatag accgattcat tgagagtggt aacaacatag cggccgtcgg
      541 gagaaacacg cgagagaaaa ccgatcgtct tatgcccagg cggttttccc gaaaacgaat
      601 tccagctaat gacatccttg gtgtcgataa tggtttcccg cgctaccggc acaatggcgt
      661 aggcaccttt gtcgcccgag ggaccgtcca cgtccatgcc gagggttgtc ccatcgtcgg
      721 aaaacgaatg gcaattggcg caggttaaaa ggttcgtaag aagaagacgg ctcttgggtt
      781 gggaaatatc tcgcagccgc cacgcaatca gcggcaaaaa ggcctcacta aggggtttga
      841 taacaccccg ctctgtggca gaaggtgcca agggcacgtc ccggtagaag atcggagcac
      901 ccactggatc gcgcgatgta ctcaatgtca cttgcccaac cgataaaggg acatgaggac
      961 gacgactgga aaagccaaca atccgcagtt gcgcgggacg ttccaccgat tgctgtttaa
     1021 cccattgcca aatgttatcg tgaaccgtcc aggactttgc cgattgctgg tacggcgtgc
     1081 cacggtaaat ctcgttggtt tcggcaatgc accgcggatc aatctcgccc tgcggtggcg
     1141 gactgccggg caccaaggcc ccaacccatg cttgcccatc tcccaccgta aactccacaa
     1201 gccatgtgtc ggccggctcg gcggaatcgt gccagaggat cgttggcgga ataatctctg
     1261 gagggaagat ggcctcgtta gggggatagt caatgaccaa tgtttgaagg tcggaagcct
     1321 tggccaaagc gctgcggaga gtgttagcca aagccaacca cttttgctcg atcttgtcag
     1381 ctaggtcggg aactcccgaa ccaactagcc actcttgagc atcgcccaag agaggatggg
     1441 g
//
LOCUS       DAOPTR010001061         1440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_69896_length_1440_cov_4.236101, whole genome shotgun sequence.
ACCESSION   DAOPTR010001061 DAOPTR010000000
VERSION     DAOPTR010001061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69896_length_1440_cov_4.236101"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..>1440
                     /locus_tag="PKI05_15745"
     CDS             259..>1440
                     /locus_tag="PKI05_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HOA53706.1"
                     /translation="MAYQLPTLPKPPKAKRKQVYLVASGDLRLSANQVCWPAQKAMED
                     ALADAVREAGYELVRAHPYKEAEGHGFISSQKEGMEVFANIDPKARLIVAEAVWQYSH
                     HVLAGLLSHQGPILTVANWSGQWPGLVGMLNLNACLTKAGKPYCTLWSEDFSAPEFRR
                     KLATWLEKGEIRHPLKHVTPLKKVKIPGSIKRLGQALADELRWKKAIMGIFDEGCMGM
                     YNAIIPDELLMPLGVFKERLSQSTLYYETTQVSDVEAREVYQWMLDRGMKFNLGSNPE
                     TDLTEDQVLTQCKMYIAALRLADDFGCHTIGIQYQQGLKDLLPASDLVEGMLNNADRP
                     PVKSRDGKRILYEGEPLPHFNEVDECAGLDALMTYRIHKAMGQPVETTLHDVRWGDWD
                     RS"
BASE COUNT          333 a          385 c          425 g          297 t
ORIGIN      
        1 cagcgattcg agccaccctc acccccgacc cctctcccgc cgagcgggcg aggtgagatt
       61 ataggatagg ctcttaccgc tctccgtaaa tccccaattg gccaccagcc ggaagcaatg
      121 caagcggctc ccttttctgc ggcgggttct tttcgctgat aggacaaata gctatcatga
      181 tacgccaagg ctgttttgtt tctcttagag tatgaagccg aggcatagcc accttttcaa
      241 atgggaagga gtcaaaacat ggcctatcaa ttacccacat tgcccaaacc acctaaagcc
      301 aagcgaaagc aggtctattt ggtagccagc ggggaccttc gcctttcggc aaaccaggtt
      361 tgctggccgg cacagaaggc catggaagac gccttggccg atgccgttcg cgaggctggg
      421 tatgagctgg tgcgggcaca tccctataag gaagcggaag gccatggttt catcagctcg
      481 caaaaggaag gcatggaggt cttcgccaac atcgatccca aggcgcggct gatcgtcgcg
      541 gaggccgtgt ggcagtattc gcatcatgtg ctggccgggc tgctcagtca ccaggggcct
      601 attttgacgg tggccaattg gtcggggcaa tggccgggct tggtgggcat gctcaacctc
      661 aacgcatgct taacgaaagc tggcaagccg tactgcacgc tttggagcga agacttttct
      721 gccccagagt ttcgccgaaa gctggcaacg tggttggaga aaggggagat tcggcatccg
      781 ctcaagcacg tgactccgct gaaaaaggtc aagattcccg ggtccatcaa gcggttgggg
      841 caagccttgg cggatgaact ccgctggaaa aaggccatca tggggatttt tgacgaaggt
      901 tgcatgggga tgtacaacgc catcatcccc gatgaactat tgatgccgtt gggtgtgttc
      961 aaagagaggc tgagccaatc cacactgtac tacgagacga cccaggtaag cgatgtcgag
     1021 gcacgggaag tgtaccagtg gatgctcgac cgcggcatga agtttaatct gggcagcaat
     1081 cccgagacgg atttgacgga agatcaagtc ctcacgcaat gcaagatgta catcgcggcc
     1141 ttgcgactcg cggacgattt cggttgccac accatcggca ttcaatacca gcaaggtttg
     1201 aaggacctcc ttcctgccag tgatttggtc gaaggcatgt tgaacaacgc cgatcgcccg
     1261 ccggtcaaga gccgggatgg taaacgcatt ctgtacgaag gcgagccctt gccgcacttt
     1321 aacgaagtgg atgagtgcgc gggtctggac gctttgatga cctatcggat tcacaaggcc
     1381 atgggccaac cggtggaaac cacgctccac gatgttcgtt ggggcgactg ggaccgctcg
//
LOCUS       DAOPTR010001062         1439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_69978_length_1439_cov_3.811416, whole genome shotgun sequence.
ACCESSION   DAOPTR010001062 DAOPTR010000000
VERSION     DAOPTR010001062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69978_length_1439_cov_3.811416"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1439)
                     /locus_tag="PKI05_15750"
     CDS             complement(<1..>1439)
                     /locus_tag="PKI05_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53707.1"
                     /translation="NQVGTVVIDWRDVRPIPVNPSVGWQLTVTPREAITIPAKVIGLP
                     PKLDFFEKVSGLIVNSTSKTALVQCTTNRPGNPITTNRLILCNLEIGVLGAILISRNE
                     TALSVYPARFGDRQYFLLQQNEFGFGNRATLTIAGYPPVASSAKKESAISLSSFSRQL
                     SWAPYEKESGLARDVKWADFADERTLITCSGTGLILIWNLGNMQPEASLQTTMGLVPA
                     ISPDGHWLAFATRDQIGILDIRKREIIALQNAPREQIFPVLAFSPTGKKLASLINHRN
                     IQIWDVASGKLEADFTLPSGIEFQGKLVFPHDDFVLGGNRYLFHWKSQLFLWDYDNIE
                     AACSLGDVTCAVVTKKRSGTADHLELVTAHIPHAAAQKVLDLALHNPETFAFHKGMPV
                     KLDLSAIPEDAREKVRHTLTQRLKEMGCEVQEEARVTLAAGVGGPKERTVSYWHSGDY
                     KVKEYTLWIRILVDGQVLWETSAGGVPFIL"
BASE COUNT          321 a          380 c          413 g          325 t
ORIGIN      
        1 aggataaacg gcacaccacc cgcggaggtc tcccacagaa cttgaccgtc caccaagatg
       61 cggatccaga gcgtatactc tttgaccttg taatcgccag agtgccagta tgagaccgtt
      121 cgttccttag ggccgccgac gccggccgcc agtgtgaccc gggcttcctc ctgaacttcg
      181 caacccattt ccttcagccg ttgggtgagt gtatggcgaa ccttttcgcg ggcgtcctcc
      241 gggattgccg acaggtcaag tttgacgggc atacctttgt ggaaggcaaa ggtctcggga
      301 ttgtgtaacg caagatcgag aaccttttgg gcagcagcat gggggatatg ggccgtgacc
      361 aattcaaggt gatctgccgt ccccgagcgt tttttagtga caacggcaca tgttacatcg
      421 cccagcgagc atgcggcctc gatattgtcg taatcccaga ggaatagttg cgacttccaa
      481 tggaaaagat aacgattgcc gccgaggacg aaatcatcgt ggggaaaaac gagtttccct
      541 tgaaattcaa tgccgcttgg caatgtgaag tcagcctcca gttttccgct ggccacatcc
      601 caaatctgga tgtttcgatg gttaatcaaa gaggctagct tttttcccgt cgggctaaag
      661 gcaaggacgg gaaaaatttg ttcccgaggg gcattttgga gggcgatgat ctcgcgcttg
      721 cggatatcga gaattccgat ttggtcccta gttgcaaagg ccagccagtg tccatccggg
      781 ctaatcgcgg gaacgagacc catggtcgtt tggaggctag cctcgggctg catattgccc
      841 aggttccaaa tgagaatcaa tcctgttccg ctgcacgtga tgagcgtgcg ctcgtcggca
      901 aaatcagccc atttcacgtc ccgggctagg ccgctttctt tttcatacgg ggcccagctc
      961 aactggcgac tgaaggacga aagggagatg gcgctctctt ttttggcgct gctggccacg
     1021 ggaggatagc ccgcaatcgt caaggtcgcg cgattaccaa aaccgaactc attttgctga
     1081 aggaggaaat attggcgatc accaaaacgc gccggataga ccgacaacgc ggtctcgtta
     1141 cgcgagatta ggatagctcc caagaccccg atttccaaat tgcagaggat gagccggttc
     1201 gtagtgatgg ggtttccggg acggttcgtc gtgcactgca ccagggccgt cttgctggtg
     1261 gaattgacga ttaagccgga caccttctcg aaaaagtcaa gtttcggcgg aagcccgatc
     1321 accttggcag ggatagtgat ggcttcccga ggtgtcacgg ttagctgcca ccccacactt
     1381 gggttgacag ggatgggcct gacgtcacgc cagtcgatga ccaccgtacc gacttggtt
//
LOCUS       DAOPTR010001063         1438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_70064_length_1438_cov_2.531453, whole genome shotgun sequence.
ACCESSION   DAOPTR010001063 DAOPTR010000000
VERSION     DAOPTR010001063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70064_length_1438_cov_2.531453"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PKI05_15755"
     CDS             <1..449
                     /locus_tag="PKI05_15755"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008682170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOA53708.1"
                     /translation="ISSVLLFTEGLLLHVSRGIRWDSDHVVILGDEVQAIANELVAGD
                     YLGRVFIGLDFFDASINRIAAWVIGMRAMLKALLAALLTPWDHLREFEDSMNYTGRLV
                     LMEAVKTLPMSAVWDYYCLKHDVPLDDAFLSDILRYENEVLSKRGH"
     gene            629..>1438
                     /locus_tag="PKI05_15760"
     CDS             629..>1438
                     /locus_tag="PKI05_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53709.1"
                     /translation="MATLPNTSAKESIFARLDKLNEFERQPVSAEKLHSGRYFAGLFA
                     GEHVAATEFVIGALFVIWGAKAHDILVGLLLGNALAVLSWTLICAPIAVQSRLTLYWY
                     LRQIGGPVLTVVYNLLNAVLYCILAGCMITVAASAVRIPFGIPPQTGVLPTDPGFVVV
                     VIIVGAVVVTLAILGFKRLSQFAVVCSPWMLSMFVCGALIALPSVGHVRNWETFLAVA
                     NERIWTGTGPKVVNVSQQGRALVLELGPIQGHLRVLSEQQVVRGADPLPIGV"
BASE COUNT          278 a          365 c          413 g          382 t
ORIGIN      
        1 agatttcctc tgtccttttg tttacagagg gcctcctcct ccacgtgagc cgcggcattc
       61 ggtgggacag cgaccacgtg gtcatcctcg gtgacgaggt tcaggctatt gctaacgagc
      121 tcgttgcggg agattatctg ggccgcgtct ttatcggtct tgacttcttc gacgccagta
      181 tcaaccggat tgcagcttgg gtcattggca tgcgggccat gctcaaagcg cttctggctg
      241 ccctgttgac gccctgggac cacctccgag agtttgaaga ctctatgaac tacaccgggc
      301 gtctggtcct gatggaggct gtcaagactc tgccgatgtc ggcggtttgg gattattact
      361 gtttgaaaca cgatgtcccc cttgatgatg ccttcctgtc ggacattctc cgctacgaga
      421 atgaggtcct atcgaagcgg ggacattgaa gccaaagagg atttctcgct ctgaaaatga
      481 tgaggcgaaa aggcttactc gcacggtgat gggtttttgt gcattttctg atggatttcg
      541 cgcacgcgct tgacaatagt cacccaaggt cctcatactt tgcagcaggt ttggctcggc
      601 gaaaggtttt cagtgaaagg ttctgacgat ggccactctg cccaatacct cggcgaagga
      661 atcgattttt gcccgtctag ataaattgaa tgaattcgaa cgccagcccg tgagtgccga
      721 gaaattgcac agtgggcgct atttcgcagg cctttttgcc ggcgaacacg tggcggctac
      781 cgaattcgtg atcggtgcgc tcttcgtcat ttggggggca aaggcccatg acattctcgt
      841 cgggctactt ctagggaatg cgctagcagt cctctcgtgg accctgattt gtgcaccgat
      901 tgcggtccaa tcgcggttaa cgctgtattg gtacctgcga caaatcggtg gaccggtgct
      961 cacggtcgta tataacttgc tcaacgccgt gctgtactgc attttggcag gatgcatgat
     1021 cacggtggca gcttccgccg tccgaattcc ttttggaatc ccgccacaaa cgggggttct
     1081 tccgaccgac cctggatttg tagtggtcgt catcatcgtc ggtgccgtag tcgttactct
     1141 ggccattctc ggttttaagc gtctctctca gtttgcggtt gtttgttcac catggatgct
     1201 aagcatgttc gtttgtgggg ccttgatcgc attgcccagt gtggggcacg ttcgtaactg
     1261 ggaaacgttc cttgccgtcg caaacgaaag aatctggacg ggaaccggac cgaaagtggt
     1321 taacgtatcg cagcaaggac gggcccttgt tttggagctt ggaccaatcc aggggcattt
     1381 gcgggttctt agcgaacagc aggtggtaag gggggcagac ccattaccca ttggtgtc
//
LOCUS       DAOPTR010001064         1438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_70075_length_1438_cov_2.098337, whole genome shotgun sequence.
ACCESSION   DAOPTR010001064 DAOPTR010000000
VERSION     DAOPTR010001064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70075_length_1438_cov_2.098337"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PKI05_15765"
     CDS             complement(<1..246)
                     /locus_tag="PKI05_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOA53710.1"
                     /translation="MKFRTKAIHLTPPGRSTGALVPAIYLSTTFAQPAPGQWGEFDYS
                     RSGNPTRKCFEDAIAELESGVGGLAFSSGMAAIHCVTM"
     gene            complement(362..1438)
                     /locus_tag="PKI05_15770"
                     /pseudo
     CDS             complement(362..1438)
                     /locus_tag="PKI05_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653306.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
BASE COUNT          314 a          393 c          382 g          349 t
ORIGIN      
        1 catggtcacg caatggatgg ccgccattcc ggaggaaaaa gccaggccac cgacaccgga
       61 ttccagctcg gctatggcat cttcaaagca cttccgtgtt gggttaccgc tgcgggaata
      121 gtcaaactcg ccccactgac ctggtgcggg ctgcgcgaag gttgtcgaaa gataaatcgc
      181 cgggaccagg gccccggtcg atcggcctgg aggcgtaaga tggatcgcct tggtgcgaaa
      241 cttcatggag agttcgccta ttaagtcaag tccactcttg ccaaatggag tcgatttacc
      301 acgatttccc taacaccttc cgcaagtatc gcgagccgaa tggcacgtgc ttcctgtggt
      361 atcatttttc aagggcttga gcgaaatcag caatcaggtc ggccgtgttt tcaattccac
      421 atgccacccg gatcatatta tcgtaaatac ccacggctcg tcgttcttcc ggtgtgtaat
      481 cgtgataact catgtaaatt ggctgctcga tcagtgattc gactccgcca aggctgggac
      541 cgatcctcgg gattttcacg gcatcgacaa ttcgcgcggt ggcgtgggcg tcggcgtctt
      601 tgacaagaaa ggtcaccaga ccgccaaagc cccgcatgta tcgcttggcc acagcgtgat
      661 ctcggtgact ttccaagccg ggataataga ccttttctac gcggggatgg ctttccaaga
      721 agcgggcgag ggccaacccg ttttcattat gccgctgcat gcgaagctcg aatgtcttaa
      781 ggcctcgcag aagcagatag atggcgtggg gggaattgcc acaccccata attcccctca
      841 ttttccgcac catttcgatg gtttcttccc gcccgataat ggccccgccg aggaggtcat
      901 tatggccacc caagtatttt gtacatgaat ggaggacaca gtcgattccg tattcgaggg
      961 gtcgcatgtt gtacggggtc gccagcgtgg catcgatgag cgtttccacg ccatatttct
     1021 tcccaagggc tgcgaaccgc tcaagatcaa ccacgctttg atgcgggtta gtgggggatt
     1081 cgctaatcaa aagtttggtt cgcttgttga tggcggcttc catcttctcg tagtcgcaag
     1141 ccgggatttg atgagtgaca atcccaatcg gcctaacacc gctgtgcaga actgccgcgt
     1201 tcggtggtag cattcatcga aaagaacgat ttcgtctccc gccttcagct ttgcgataag
     1261 gtagcaaacg atggccgcca ttcccgtggc aaacgtaaca caggcttccc caccctctag
     1321 ggcggccaat ttgcgttcta aaaccttttc cgtgggattg ccataacggc catactcttc
     1381 tcgaggaagc ttttttgtta catactcgat cagcgaggcc gtgttctcga aagtgtaa
//
LOCUS       DAOPTR010001065         1435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_70291_length_1435_cov_2.542029, whole genome shotgun sequence.
ACCESSION   DAOPTR010001065 DAOPTR010000000
VERSION     DAOPTR010001065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70291_length_1435_cov_2.542029"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1223
                     /locus_tag="PKI05_15775"
     CDS             <1..1223
                     /locus_tag="PKI05_15775"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOA53711.1"
                     /translation="AYGDIMAAVCRSDGDWTKRREANRIHRPRGQSQNLFTMICPRCH
                     TWLSQVKRPQGLVYQCASCRGFAVSIPVLRKLVPQGIIRDLWNRAQEKQSRSGAECPV
                     CRRKMADVAVGGMEKAPIIDVCIRCQFVWFDRRELEQLPPVIVKQPDRETLPLEVREK
                     LAVLELERLKRREQLATAGDQYPEEWWQWIPGLLGLPVELDAPAVRSVPWLTWSITAA
                     MVVTFLLTFNHLETILDVLGLKPAEFMRLGGLTLITSFFLHGSVWHLLGNGYFLLIFG
                     DQVEDDLGQWRFAALLALAALAGDILHILGDPRSTVPCIGASGGISGIIAFYALRFPS
                     ARLGIMLRYFLIFRWIQFPAWAGFVAWLVLQIILAIEQVQGVTNVSALAHLGGCLVCV
                     GAWGLWQSGLLKAG"
BASE COUNT          288 a          381 c          419 g          347 t
ORIGIN      
        1 cggcctacgg ggatataatg gcggcggttt gccgatcgga tggagactgg accaaacggc
       61 gcgaagcgaa tcggatacac cggccgcgcg gccaatcgca aaacttattc acaatgattt
      121 gcccacgctg ccatacctgg ctaagtcaag tgaagcgccc tcaggggttg gtgtatcaat
      181 gcgcttcatg tcggggattt gccgtctcga tacccgtgtt gcggaagctc gttcctcagg
      241 gcatcatccg agatctttgg aaccgtgccc aggaaaaaca gtcccgctct ggtgccgaat
      301 gtcccgtttg ccgccgcaag atggctgacg tcgctgtcgg cgggatggag aaggcaccca
      361 ttattgatgt ttgcatccgg tgtcaattcg tttggttcga taggcgagaa ctcgagcagt
      421 tgccaccggt aatagtgaaa caacctgacc gggagaccct gccgctagag gtgagagaaa
      481 aattagccgt cttggaactg gagcgactca agaggcgaga gcagttagcg actgcaggcg
      541 accaataccc ggaggagtgg tggcaatgga tccccgggct tctcggattg ccggtggaac
      601 tggatgcacc cgctgtccgc tctgtgccct ggctgacgtg gtccattacg gccgccatgg
      661 tggtgacgtt tttgctcacg tttaaccacc ttgaaaccat cctcgatgtg cttgggctca
      721 aacctgccga gtttatgcgg ctcggcggat taaccctaat cactagcttt tttcttcatg
      781 ggagtgtttg gcatcttttg ggcaacggtt attttctcct catctttggg gaccaagtag
      841 aggacgatct agggcagtgg cgttttgcgg cgttattggc cttggccgca ctggcaggcg
      901 atattttaca tatcctcggt gaccctcgca gtacggtacc atgtatcggg gccagtggtg
      961 gtatttcggg gatcattgcg ttttacgcct tgaggtttcc cagcgctcgt ctagggatca
     1021 tgttacgtta ttttttgatc tttcggtgga ttcaattccc ggcctgggct ggctttgtcg
     1081 cctggttggt tcttcagatc atcctggcca tcgaacaggt tcagggcgta accaacgttt
     1141 cggcactcgc ccacttaggt ggttgcctcg tctgtgttgg ggcgtggggc ctttggcaga
     1201 gcggcttgct aaaggcgggt taaagctcgc gaagctctcc ccgacccctt gactggcgat
     1261 ttgaaatgcc aatgggagaa ccaaatagca atctccccct acgcccgtaa gcgaccgctg
     1321 cccataaagc cgtcccacct ggtgggcaat caagatacac cagcccgagc aaggcgcaaa
     1381 aacggccgac cacgaggggc tcccggcaat tatatctcac aaatttggcg catgg
//
LOCUS       DAOPTR010001066         1434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_70334_length_1434_cov_3.870921, whole genome shotgun sequence.
ACCESSION   DAOPTR010001066 DAOPTR010000000
VERSION     DAOPTR010001066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70334_length_1434_cov_3.870921"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PKI05_15780"
     CDS             <1..240
                     /locus_tag="PKI05_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53712.1"
                     /translation="GMGTATFLLLSGVPFLVRDFLANSSPVISDDVTTGEFWEFTTFN
                     GESYFRSGCYCRSLGLYSVAPATSPRCNGQFRGEK"
     gene            260..>1434
                     /locus_tag="PKI05_15785"
     CDS             260..>1434
                     /locus_tag="PKI05_15785"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOA53713.1"
                     /translation="MSNSSTFARLTPIFLCLWLSGIGLGLGWIGLQRWSIRKLLRTAE
                     PISNTSLQSWLTVQAKRLGFTRRLRVIRCGGLQTPVAFGIVRPTIATPKTFRVEPLDV
                     RSQAILLHELSHLAGSDPFWNLLAALVKALLWWHPMAWIAAKRLKELCEEAADEVTVL
                     LPNGPRELAAGLLECARGRFQTRMPVLAALRTSHARSFIARRIHRLLALEKKDDKAAR
                     VGRIKRIQRFVVITTALAVFLASLGFPVPEVLLTKGEVAMRHRHSLWRHSLMAAAIAA
                     FATPWGMQTLAAEGPGPDQPREVVRQERPDAPRERAAVEGERGPREGDEVTRAEGEGR
                     ERAEGRGPREEGRGPRGPEAIAARLEELRRALREAEEAGQMDRVEQIRREIRSLEAQL
                     "
BASE COUNT          297 a          359 c          455 g          323 t
ORIGIN      
        1 ggcatgggca cggcaacttt cctgttacta tcgggtgtgc cgtttcttgt ccgtgatttt
       61 ttggcaaaca gtagcccggt gatttccgac gatgtcacca ccggcgaatt ctgggagttc
      121 acaactttta acggagaaag ttactttcga agcgggtgtt actgtcgatc attgggcctc
      181 tactcggtgg cgccagcaac ttcaccaagg tgtaacggtc aattccgggg agaaaagtga
      241 aaaagacatc actagtcctg tgtcgaattc ttctaccttc gcccggctca cgcctatatt
      301 cttgtgcctt tggctatctg ggattgggct ggggcttggc tggattggcc tccaaagatg
      361 gtcgatccgg aagctgctgc gaactgccga accgatctcg aatacctcgc tccagagttg
      421 gttgacggtg caagcaaagc gattaggttt tacccggcgt ctgcgggtga ttcgttgcgg
      481 cggtttgcag actccggtgg cgtttggcat cgtccgtccg accattgcta cccctaagac
      541 ttttcgtgtc gaaccattgg acgtgcgcag ccaggcgatt ttgctgcatg agttaagtca
      601 tcttgcgggt agtgatccct tttggaactt gttggcggct ttggtcaaag ctctcctctg
      661 gtggcacccg atggcctgga tcgcggcgaa acgtttgaag gaactgtgcg aagaagcggc
      721 ggacgaagtg acagtccttt tgcccaatgg cccacgggag ttggccgccg ggctgcttga
      781 atgtgctcgt ggtcgatttc aaacgcgtat gccggtactt gcagcacttc gaacgtcaca
      841 tgcgcgttca ttcattgccc gacgtattca ccggcttctt gccctggaga aaaaagacga
      901 taaggcggcc cgggtaggga ggatcaagcg aattcagcga tttgtcgtga taacaacagc
      961 cttagctgtg ttcttggcgt cgttaggatt tcctgttcca gaggttttgt tgacgaaagg
     1021 agaggtagct atgcgacatc gacactcttt atggcggcac tcactgatgg ccgcggcaat
     1081 tgcggcgttc gccacgccgt ggggcatgca aaccctggcc gcggaaggcc cagggcccga
     1141 tcagccgcgg gaagttgtcc gccaagagcg gccggatgcc ccgcgcgaaa gagcggctgt
     1201 ggagggagaa aggggacctc gcgaaggtga cgaggtgaca cgtgccgagg gtgaagggcg
     1261 agaaagggcc gaggggcggg gtccacgtga agaaggccga ggaccacgag gaccagaggc
     1321 aatcgcggca aggcttgaag aactacgacg agcattgcgc gaggcggaag aagccggtca
     1381 aatggatcgc gtagaacaga ttcgccgcga gattcggtcg cttgaggctc agct
//
LOCUS       DAOPTR010001067         1432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_70485_length_1432_cov_3.179375, whole genome shotgun sequence.
ACCESSION   DAOPTR010001067 DAOPTR010000000
VERSION     DAOPTR010001067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70485_length_1432_cov_3.179375"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /locus_tag="PKI05_15790"
     CDS             complement(<1..1337)
                     /locus_tag="PKI05_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53714.1"
                     /translation="MTRVKRPLWLTKSLGLIAGLAVVFSALPVLAGAPAVVVRLAPLA
                     QWQQNAAFLADMLKEHPSSVRIQMLVGLLDAVGKNGVDPTRPMGLACLVGEQGNPILV
                     GCIPTSNLDQLHTFAKDWISEPTLISDNVYHVKVRDRELYVRLYDAWVVVSHNAEILS
                     SLSGDPAMWLAEFDSSMDVSIAINPRELASLRGVFRNWLERQEALAAMLSRIEVSQEV
                     RQSVLRPLLETAKEAVQQTEKVILSYRIDRNEKKLVGNCTWTAKAGSDLAAQFAQERL
                     VTSPLVAIRLWNDTAMTMAWSVTFPPPSEEDLERIGSLIQSRWDKQIERRISNSVDAQ
                     VAKNLAHDFLGLLGDWAREGKADGIVTLVTKPDQVTLLIGGYVGNSTGLEAWVGNVVE
                     SAQQRAPKAFERVTIIPDHTLHGDASIHLMRIALGDRLSPEQKAVLGDDLEVA"
BASE COUNT          308 a          418 c          381 g          325 t
ORIGIN      
        1 aaggcgacct caagatcatc tcctaagacc gccttttgct cgggggaaag cctgtcgcca
       61 agggcaatcc gcataaggtg gatgctggcg tccccatgta gcgtgtgatc ggggatgatc
      121 gtgactctct caaaggcctt gggggcgcgc tgctgtgccg attcgactac gttacccacc
      181 caggcctcaa ggcccgtgga gtttcccaca tacccgccaa tcaacagcgt tacttggtcc
      241 ggtttggtaa ccagtgtcac gatgccatcg gctttgcctt cacgtgccca atcgccgaga
      301 agccccaaaa aatcgtgggc cagatttttg gccacctgtg cgtccacgga attagatatg
      361 cgacgctcaa tttgcttatc ccagcggctc tgaatgaggc ttccaatccg ctcgaggtct
      421 tcctcggatg ggggtgggaa ggtcaccgac caagccatcg tcatggccgt gtcgttccaa
      481 agccggatag caaccagtgg gctagtaaca agtctttctt gggcgaactg tgcggccaaa
      541 tcagaacctg ccttggccgt ccatgtgcag tttcccacga gtttcttctc atttcgatcg
      601 attcgatagg acaagatcac cttctcggtt tgttgaaccg cttcctttgc agtttcaagg
      661 agcggccgaa ggacgctttg ccgaacctct tgagaaactt caattctcga gagcatcgcg
      721 gcgagcgctt cttgccgttc gagccaattc ctaaaaactc ccctcagcga cgccagttcg
      781 cgggggttga tggcgatcga cacatccatg gaagagtcga attcggccag ccacattgcg
      841 ggatctcccg atagcgagct gaggatttcc gcgttatggc ttacgacgac ccaggcatcg
      901 taaagcctga cgtagagctc tcggtctcga accttcacgt ggtaaacgtt atccgagatc
      961 aatgtcggtt cgctgatcca gtccttggcg aaagtatgaa gctgatccaa atttgaggtg
     1021 ggaatgcacc caaccaggat ggggttgcct tgttcaccga caagacacgc caagcccatc
     1081 ggtctggtgg gatcaacacc gttcttcccc acggcgtcaa ggaggcccac aagcatctgg
     1141 attctgacac tgcttgggtg ctctttgagc atgtccgcca aaaacgcggc gttctgctgc
     1201 cactgggcga gaggggctag tcgcacaacg acggccggtg ccccagccaa gacgggcagg
     1261 gcgctgaaaa cgaccgccag tccggcgatt aggcctaagc tcttggttag ccacaaaggt
     1321 cgtttcactc ttgtcattcc tcgtctcctc ctccaattgt ggacccacga acatctacct
     1381 gtaacaacgc tggctaccaa tacccctttc aaagagtggc tggcctacag tt
//
LOCUS       DAOPTR010001068         1432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_70489_length_1432_cov_2.923021, whole genome shotgun sequence.
ACCESSION   DAOPTR010001068 DAOPTR010000000
VERSION     DAOPTR010001068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70489_length_1432_cov_2.923021"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /locus_tag="PKI05_15795"
     CDS             <1..1253
                     /locus_tag="PKI05_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOA53715.1"
                     /translation="FESLLTEGAEILTESVSKAFLEAYGIPVTKPFLARTPDQAVELA
                     KRIGFPVVMKIHSPQITHKTDVGGVILNIANEQGVREAFDRLVRRAQEKRPDAQILGC
                     TVQQMVSYPNSFELIIGTKRDPVFGSVILVGMGGVAAEVFQDRALALPPLNETLARRA
                     LESLKSWPLLRGYRGRPGVNIDKLIEVLMRFSYLVADYPEIKELDVNPLLATAEEVIA
                     LDARVVVDRDILAHGVRPYAHLAIRPYPEEFVTERKLRDGTPVILRPIKPEDEPMWHE
                     LLASCSTQSIWFRFSYLFKHTTHEMASRYCFIDYDREMGIVAEVEEDGQRRLIGVGRL
                     VADANHETAEYAVIVADRWHGRGLGGLLTDYCLEVARRWGIKKVVAETSKDNARMLAT
                     FRNRGFEFNEKADQDVVLVSKTLA"
BASE COUNT          319 a          371 c          423 g          319 t
ORIGIN      
        1 ttttcgagag cctgctcacc gagggtgcag aaatcctcac ggaaagcgtt tccaaggcgt
       61 tcttggaagc gtacggtatc cccgtgacca agccgttctt ggcacgaact cccgatcaag
      121 cggtggaact tgccaagcgg atcgggttcc cggtggtgat gaagattcac tcaccacaaa
      181 tcacgcacaa gaccgacgtc ggcggcgtta ttctcaacat cgcgaacgaa caaggcgtac
      241 gagaggcctt cgatcgactg gtccgtcggg cccaggagaa acggccggat gctcaaattc
      301 ttggctgcac agttcagcag atggtcagct atcccaacag tttcgaactg attattggga
      361 cgaagcgaga tcctgttttc ggttctgtga tcctggtcgg aatgggtggg gtcgctgcgg
      421 aggtcttcca agaccgggca ttggcccttc ctccccttaa tgaaacgttg gctcggcgtg
      481 ccctggagtc gctcaagtcg tggcccctgt tgcgtggtta tcgtggtcgg cccggcgtca
      541 atatcgacaa gttaatcgag gtacttatgc ggttctccta tctggtggcc gactatcccg
      601 aaatcaagga actggatgtt aacccgctgt tggccactgc agaagaggtt attgcgttgg
      661 acgcgcgggt tgtcgttgat cgggatattt tggcccacgg tgttcggccg tatgcccatc
      721 tggcaattcg gccatatccc gaagagttcg ttacggaacg aaaactcaga gatggtacgc
      781 cggtgatcct ccggcccatc aagcccgaag acgagcccat gtggcatgaa ttgctggcga
      841 gctgttccac gcagtcgatt tggttcagat tcagttacct gttcaagcac acgacacatg
      901 aaatggcgtc caggtactgc tttatcgact acgaccgaga gatgggcatc gtggccgagg
      961 tcgaagagga tgggcagcgc cggcttatcg gtgtggggcg tctcgtggcc gatgccaatc
     1021 acgagacagc agagtacgct gtgatcgttg ccgaccgctg gcacggtcga ggtctcggcg
     1081 gcctgctcac ggactattgc ctggaggtcg ccaggcgatg gggtatcaag aaggtcgtgg
     1141 cagaaacctc gaaggacaat gcccgaatgt tagccacctt ccggaaccgt ggattcgagt
     1201 tcaatgaaaa agccgaccaa gacgtggtgc tcgtttccaa gacattggca tagtaggcta
     1261 cgagttcacc gctgattatc gacagtaacc gcacagccca cgttgtgggc tgtgtttttt
     1321 tgtggtcatg gactaccaaa gcttgagtag cccagtgttt gggatgaaat tgacaatgat
     1381 ccaatagccc agtcccagac ctaacaaggc agaaatgaca aagccaaggg cg
//
LOCUS       DAOPTR010001069         1430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_70612_length_1430_cov_3.101818, whole genome shotgun sequence.
ACCESSION   DAOPTR010001069 DAOPTR010000000
VERSION     DAOPTR010001069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70612_length_1430_cov_3.101818"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PKI05_15800"
     CDS             complement(<1..216)
                     /locus_tag="PKI05_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOA53716.1"
                     /translation="MTIQPQTTGASLQLAKPGEPVTGAEIVVQSLVNHGVEVVFAYPG
                     GASMPIHQAFTRFRDKIRVILPRHEQGG"
     gene            complement(627..1331)
                     /locus_tag="PKI05_15805"
     CDS             complement(627..1331)
                     /locus_tag="PKI05_15805"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOA53717.1"
                     /translation="MNHEDFDVHGLATYLHLTPQQVQRLADRGKIPGRKVAGQWRFSP
                     AEIHQWLEHRIGLLTDDEDFAEMESQLKPKNATAGEISIAALLPVEAIQVPLVGRTRN
                     SIVREIVEVAARTGWLWDTQAMIEAVLAREDMHPTALENGVALLHPRRPQPRILEQPF
                     LAFGRTQSGIPFGGDTGTLTDCFFLICSVEDRGHLRTLARLSRLLAVPEFLPTLRELE
                     DPSAIRKWIAEVEKGF"
BASE COUNT          314 a          408 c          385 g          323 t
ORIGIN      
        1 accgccctgc tcgtgacgcg gcaggatgac gcggatcttg tcgcggaacc gcgtaaatgc
       61 ctgatggatg ggcatgctgg ccccgcccgg gtaggcaaat acgacctcca ccccgtggtt
      121 gaccaacgat tgaacaacaa tctccgcccc agtgacaggt tcgccaggtt ttgccaattg
      181 caagctcgct ccggtcgttt gaggctggat ggttatcgtc acactcagtt ctcctaaaac
      241 tttctccgac aatgcagaaa cgcgttctgt gatgccgatg ccttagccta acaactatgc
      301 cgcggccgct tggtctattc taccgcgcac tcaatcagcc ccttcttaat acccacaacg
      361 ggggaagaat catctcaaag cgtgcggcca ggaccgcaag tcgccgattg tcaagttttt
      421 tgaaataaaa tgccgatttc cttaagcctt tgctcgtgtc caatcctctg cccatccctc
      481 agcatacgga taatactgcc ttttttcaac gaaggcatga tccaggttcg gcaacccttc
      541 acgccgaagt ccataaatac attattgccg atgccggggg ccctgcggtt agagattgcc
      601 aaccgatcac tttggttcgc gcgggatcag aatcccttct cgacttcggc gatccacttg
      661 cgaatggccg acgggtcttc caattcgcgc aaggtgggca ggaattcggg gacggccaac
      721 aggcggctca agcgggccaa ggtccgcaga tggccacggt cttccactga acagatgaga
      781 aagaaacagt cggttaaggt cccagtatcg ccgccgaagg ggataccgct ttgcgttcgg
      841 ccaaaggcca gaaacggttg ttcgagaatc cgcggctggg gacggcgcgg gtgcagaagg
      901 gccacgccgt tttccaaggc tgttgggtgc atatcttcgc gggcaagaac cgcttctatc
      961 atagcctggg tgtcccagag ccagcctgtc cgggcggcaa cttccacgat ttcacggaca
     1021 attgaatttc gcgtgcggcc taccaagggg acctgaatgg cctcgaccgg caggagggcg
     1081 gcaatggaaa tctcgcctgc cgtcgcattc ttcggtttga gctgcgattc catttcggcg
     1141 aaatcttcgt catcggtcaa taatccgata cgatgttcca accattgatg gatttccgcc
     1201 ggtgaaaaac gccattgccc ggccaccttt cgtccgggaa tcttcccccg atcggcgagc
     1261 cgttgcacct gttgcggcgt caggtggaga tacgttgcaa gtccgtgaac atcaaagtct
     1321 tcgtgattca tacaaagtcc gtaagaagtg gaaggtgcta tcgcaaaaac gcctggctcg
     1381 ggctatttcc ttcgaaatcg gcaagcgtta atatatggat tttacggtcc
//
LOCUS       DAOPTR010001070         1430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_70614_length_1430_cov_3.008727, whole genome shotgun sequence.
ACCESSION   DAOPTR010001070 DAOPTR010000000
VERSION     DAOPTR010001070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70614_length_1430_cov_3.008727"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1430
                     /locus_tag="PKI05_15810"
     CDS             <1..>1430
                     /locus_tag="PKI05_15810"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOA53718.1"
                     /translation="VGILCLPELCITGYGCEDAFHSPSLHRTALEVLHECAAATKGML
                     VSVGLPLFHQSAVFNCACLLADGKIVGFVPKRYLASEGLHYEPRWFRPWPAGVRDRIH
                     IGGQWYPIGDYVFDVGGIRIGFEICEDAWVPHRPGAELAAHGVDIILNPSASHFSFGK
                     HEIRKRFVVEGSRSFRVGYVYANLLGNEAGRVIYDGGVLIATAGQLLAAGPRFAFQPV
                     VVTAASVDVEMNRMSRARSAGFQPGLALDRDVTISIPWSYPDIEPRFEHPHVASWELG
                     PHVKEEEFTRAVMLGLFDYLRKSRAQGFVVSVSGGADSSAVACLVALMIEASYNELGH
                     EAFCQALSHIPELRTTKSHQQRISRLLHGLYQATRNSSEATRDAATTLVTSLGGTMSC
                     ISIDEIVHKYVRTIEEVLGFKLNWNDHDLALQNIQARARGPSAWLLANVKKALLLTTS
                     NRSEAAVGYATMDGDTCGCLSPIAGI"
BASE COUNT          277 a          372 c          428 g          353 t
ORIGIN      
        1 aggtgggcat cctctgcctg cccgagcttt gcatcaccgg ttatggatgt gaggatgctt
       61 ttcatagccc gagcctccat cgaacggccc tcgaagtcct tcacgagtgt gctgccgcca
      121 ccaagggaat gctcgtgagt gttgggctgc cactctttca tcagtctgct gttttcaatt
      181 gcgcgtgcct gttggctgac gggaagattg tcggcttcgt tcccaaacgg tatctggcat
      241 cggagggcct tcattacgaa ccacggtggt ttcgcccatg gcctgccggg gtccgtgacc
      301 gaatccatat tggaggacag tggtatccga ttggggacta tgtgtttgac gtgggtggga
      361 ttcggatcgg ttttgaaatt tgcgaagatg cctgggtccc gcaccgccca ggggcagaat
      421 tggccgccca cggtgttgac atcatcctta atccaagtgc gagtcatttt tcctttggca
      481 aacacgaaat ccggaaaaga ttcgttgtgg aaggttcacg gtcgtttcgg gtgggttatg
      541 tttatgccaa tttgctcggc aacgaggcag gacgtgtcat ctacgatggg ggtgtcctta
      601 ttgccacggc ggggcaatta ttggcggctg gtcctcgatt tgcctttcag ccggtggttg
      661 ttaccgcagc gagcgttgac gtggaaatga atcggatgag tcgggcgagg tcagccggtt
      721 ttcaacctgg cctggccttg gatcgtgacg ttacgattag tatcccctgg tcgtatcccg
      781 acattgaacc acgatttgaa catccccacg ttgcctcgtg ggaattgggg ccgcacgtca
      841 aagaggagga atttacccgt gccgtgatgc ttgggctttt cgactatttg cggaagagtc
      901 gggcacaggg ctttgtcgtg agcgtgagtg gtggcgccga ctcgtcggcc gtggcgtgcc
      961 ttgtcgccct gatgatcgag gcctcgtaca acgagctcgg tcacgaggct ttttgccagg
     1021 cgctgtcgca tatccctgaa ttgcgaacga ccaaaagcca ccagcaacgc atttcacgcc
     1081 tcttacatgg gttgtaccag gctacccgca atagcagcga ggccacgagg gatgctgcca
     1141 ccacactggt gacgtcccta gggggcacca tgtcgtgcat ttccattgat gagatcgtcc
     1201 acaagtatgt gcgaacgatc gaggaggtgc tgggattcaa actcaactgg aatgatcacg
     1261 atctcgccct ccaaaatatt caagcccgag cgaggggtcc gagtgcctgg ctcttggcga
     1321 atgtcaagaa ggctttactc ttgacaacca gcaatcgttc cgaagcggcc gtgggttatg
     1381 ccaccatgga tggcgatact tgtggctgtc ttagccccat cgccggaatc
//
LOCUS       DAOPTR010001071         1426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_70901_length_1426_cov_2.819110, whole genome shotgun sequence.
ACCESSION   DAOPTR010001071 DAOPTR010000000
VERSION     DAOPTR010001071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70901_length_1426_cov_2.819110"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..>1426
                     /locus_tag="PKI05_15815"
     CDS             259..>1426
                     /locus_tag="PKI05_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA53719.1"
                     /translation="MLHPLRSLLTVLGIFIGVASVIWLLAIGEGISRKAQEQIESLGA
                     TNIIIRSIKPPSEQVQTSGLVLQYGVTRADYARLVETIPTVVRAVRIRELRQRFSYNG
                     RMVDGRLVGCTPEYQDIVRLEIDRGRFIQDSDLQDLKNYCVLAAGTAEKLFPFEDPLG
                     KVIRVEEHFYTVVGVCKPKMPSAGIGGSFAAQDFSSDVYIPITTLWWRIGDVVFTRRA
                     GSTEAEQIELSQITLQVDKTANVVPTADVVKATLAKYHPIEDYGVTVPLELLEQARTT
                     RMMFMVFMGLIAGISLVVGGIGIMNIMLATVTERTREIGIRRALGAKRRDIIRQFLVE
                     TVVLSTLGGISGILVGFLAPLLSEGAIWALQFLAPRLYNSLPEIVKTMRPVVVPA"
BASE COUNT          297 a          399 c          408 g          322 t
ORIGIN      
        1 gggaaaaacg atcatcttgg ttacccacga gaacgccgtg ggcgaagggc gcggaggatt
       61 attcgtctca aagacggacg catcgaatcg attattgata acccccaggc caagccacgg
      121 agcgagctgc cagaagacgt tgccaaggcc aaagcaccac aataacttgc tttccccaag
      181 gaatgcagcc acacatccca gtaagaaacc atgttgatcc tttggtatag gacgatccaa
      241 ctaggactca agagcttgat gctccacccg ttacggtcac ttttgaccgt attgggtatc
      301 ttcatcggtg tggcgagcgt catctggttg ctcgccatcg gtgaggggat tagtcgcaag
      361 gcccaggaac aaattgaaag ccttggggcc accaatatta tcattcgctc gatcaagccg
      421 ccgtccgagc aggtgcagac gtcgggcctt gtccttcagt atggcgtcac ccgcgcagat
      481 tatgcccgac tcgttgagac cattcccacg gttgtgcggg cggtccgtat tcgggagctg
      541 cgccagcgat tcagttacaa tggccgaatg gtcgatggac gcctggttgg ttgcacgcct
      601 gaataccagg acatcgttcg tttggaaatc gaccgcggac gttttatcca agattcggac
      661 cttcaggacc tcaagaacta ctgtgtcctt gcggcgggga ccgcggagaa gctctttcct
      721 tttgaggacc cattgggtaa ggtcatccgg gtggaagaac acttctatac cgtggtcggc
      781 gtgtgcaagc caaagatgcc ctcggctggg atcggtggct cttttgccgc ccaggacttc
      841 tccagcgatg tgtatattcc catcacgacc ctttggtggc gcatcgggga cgtcgtcttc
      901 acccgccggg ccggctccac ggaggccgag cagatcgaac ttagtcaaat caccctccag
      961 gtcgataaga ccgccaacgt cgtccccacc gccgatgtcg tcaaggcgac gctggccaaa
     1021 taccatccca tcgaagatta cggtgtgacc gtccctttgg aactgcttga gcaggcacgc
     1081 acaacgcgga tgatgttcat ggtcttcatg gggttgattg ccgggatttc gttggtcgtg
     1141 ggcggcatcg gcattatgaa catcatgctg gctaccgtca cggaacgaac gcgtgaaatc
     1201 ggcatccggc gtgccctggg tgccaagcgg cgagacatca tccgccaatt cctggtggaa
     1261 accgtggtcc tttccacgtt ggggggcatt tcgggcatcc tcgtcggttt cttggctccg
     1321 cttttgagtg agggagcgat ctgggcattg cagttccttg ccccgcgact ttataactcg
     1381 ctgcccgaaa ttgtcaaaac catgcggccg gtggttgttc cggcgt
//
LOCUS       DAOPTR010001072         1425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_70969_length_1425_cov_2.569343, whole genome shotgun sequence.
ACCESSION   DAOPTR010001072 DAOPTR010000000
VERSION     DAOPTR010001072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70969_length_1425_cov_2.569343"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="PKI05_15820"
     CDS             <1..671
                     /locus_tag="PKI05_15820"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOA53720.1"
                     /translation="AVLRSRGVSVVSLGEEGEDDHDHDHADHHGGEEQHQDVSDDPHE
                     HEHGRHVWLSPRRLSRQAEKIAEALCRVDPSGAVGYQKNLVKTQEELRELDRELSQKL
                     EPLRGQTFFVFHPAWECFAEDYGLIQQAVQVQGKEPSDHEMTELQRKARQTKLKVILV
                     QPQFSSRAAEAIAEVAGLQVVVADPLTEEIPTVLRQVADLLVSAYRETYGAARTETPS
                     TGQP"
     gene            684..>1425
                     /locus_tag="PKI05_15825"
     CDS             684..>1425
                     /locus_tag="PKI05_15825"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA53721.1"
                     /translation="MLSNPSSDQKLGSESAPHPSVAASEEIVVQVEGVSFTYAQQNGA
                     GSATKWVLEDVSLKVFADDFLGIIGPNGGGKTTLLKIILGLLEPQKGWVRVFGRTPRE
                     VRHQIGYVPQHARIDATVTATVLDIVLAGRIARSRWGFRYGKRDREAAFVALEMTGVA
                     PLAHRRIGTLSGGQRQRVLIARALAAEPKLLLLDEPTAGIDPYVEQNLTDLLHQLNES
                     LPIVVVSHDIGFVSTHLKRVACLNRRLTV"
BASE COUNT          310 a          368 c          439 g          308 t
ORIGIN      
        1 aagcggttct ccgcagccgt ggtgtaagtg tggtctcgct cggggaagaa ggggaagatg
       61 accatgacca tgaccatgcg gaccaccacg gcggggagga acagcatcaa gatgtcagcg
      121 acgacccgca cgagcatgaa catggtcgtc atgtctggct tagtccccgg cgccttagcc
      181 ggcaagcaga gaaaatcgcc gaggccctgt gtcgtgttga tccatcgggt gccgttgggt
      241 accagaaaaa tctggtgaag acacaagaag aattacggga gttggatcgc gagttgtcac
      301 aaaagctcga gcctctccgc ggtcagacgt ttttcgtttt tcatccggcg tgggaatgtt
      361 ttgccgagga ttatggcctt attcagcaag cagttcaagt gcaagggaag gagccttctg
      421 atcacgaaat gaccgagtta cagcggaagg ctcgtcagac gaagctgaag gtgattctgg
      481 tccagccgca attctcatca cgtgcggccg aggccattgc cgaagtagca ggattgcaag
      541 tggtcgttgc cgatcctttg acagaggaga tccccaccgt actgcggcag gttgcagatc
      601 tcctcgtctc tgcctatcgt gaaacttatg gggcagcgcg cacggaaacc cctagcacgg
      661 gccagccctg agaaggtgtc gaaatgttat caaacccttc ttccgatcag aagttgggtt
      721 ctgaatctgc gccccaccca tcagtagcgg cgagcgagga gatcgtcgta caggtggaag
      781 gggtaagctt cacgtatgct caacaaaatg gggcgggttc ggcgacgaag tgggttttag
      841 aagatgtctc cctcaaggtg ttcgccgacg attttttggg aatcattggg ccgaacggcg
      901 ggggaaagac aaccttgttg aagatcatcc ttggcctttt ggaaccgcaa aagggatggg
      961 tcagggtgtt cggaagaacc ccgcgcgagg tacgtcacca aattgggtat gtcccccaac
     1021 acgcccgcat tgatgccacc gtgacagcga cggtgctgga catcgttctt gcgggtcgaa
     1081 ttgcccgttc gcggtggggc tttcgatacg ggaagcgaga tcgcgaagcg gcctttgtgg
     1141 ccttggaaat gacgggtgtt gcgccactgg cgcatcgtcg tattgggact ctctccggtg
     1201 gacagcggca gcgggtcctg atcgcccgcg ccctggctgc cgagccaaaa ctgctcctgc
     1261 ttgacgagcc tactgcgggt attgatccct atgttgagca aaatctcacg gaccttttgc
     1321 atcaactgaa tgaaagcctc ccgatcgtgg tcgtgagtca tgacatcggg tttgtttcga
     1381 cgcatctcaa gcgcgtggcc tgcctgaatc ggcggctcac agtgc
//
LOCUS       DAOPTR010001073         1423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_71088_length_1423_cov_4.074561, whole genome shotgun sequence.
ACCESSION   DAOPTR010001073 DAOPTR010000000
VERSION     DAOPTR010001073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71088_length_1423_cov_4.074561"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            394..>1423
                     /locus_tag="PKI05_15830"
     CDS             394..>1423
                     /locus_tag="PKI05_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53722.1"
                     /translation="MTARQMRRHANEDQGGSAPAPGPDALASGSPSVAQAGSPENQEE
                     LPPGVVATAEWLVSFCHEALLQQQTPEALAARAYLQMQGLAWSGIADLPIGVLNDASK
                     ARDIYQDRQMGSVEDIEYWLSDARLSRVLLGPIRDGRGKLVTLWARPVEPTQRTLLYR
                     RPWQERVAVYGAECLSTVDPVPVYVVEKILDALVLRSHKIQPAVAFGRRFDQVPAQSW
                     AWLGRNVAGPIVLIPVGSHIPSTIFRRVRIQVERLIDPPEIWVLPPKRMFAPLGRMAA
                     VLKSPEFQEFIRDRAVPLLGRKRRIQLSPGVAAMHPHSANPESDHYLRPRQEGAEIAP
                     QERREDLRS"
BASE COUNT          258 a          420 c          438 g          307 t
ORIGIN      
        1 cctcacccca accctctccc ggagggagag ggggactttt tgggataggc tcttatcgaa
       61 ggaccgctcg ggtaaaacga acgaaacgac catcgtcctt gctgatcggc agatcggacc
      121 gcaaggacct acttactccc agagccagaa tgttccggtc ctttgcgtgc gctagcacgg
      181 agtaacagcg ttcgcgcttt ccaaaaaact cgaaaatagc aagctgcgca ccagtttggc
      241 gtttgcccca tctggccttc ccgggatggg cgacctcccg gacgaacacc cgtgtcttgt
      301 ccgccattcg attgctaaaa tggcgttgac tcctcggctg tctttggcct gggtgttgtc
      361 cgatagcgac taccaggagg agggcttttc tctatgactg cgcggcagat gcgccgacac
      421 gccaatgaag accaaggcgg ttcagctccg gcaccggggc cagacgcttt agcatctgga
      481 agcccctcgg tggcgcaagc cggttcaccg gagaatcaag aggagcttcc gcccggcgtt
      541 gtcgccaccg ctgagtggtt agtctctttt tgtcacgagg cccttttgca acagcaaacc
      601 cccgaggccc tagccgcgcg ggcatatctc cagatgcagg gcctagcctg gtcgggcatc
      661 gccgatttac ccattggcgt tctgaacgat gcgagcaagg cccgagatat ctaccaggat
      721 cgccagatgg gttctgttga ggacatcgaa tattggctca gtgatgcccg gttaagtcgt
      781 gtccttcttg ggccgatccg ggatggtcgc gggaaactgg tgacgctttg ggcacgaccc
      841 gtcgagccaa ctcaacggac ccttttgtac cgtcgcccgt ggcaggaacg cgtcgccgtt
      901 tatggggccg agtgtctttc tacagttgac cctgttcccg tttacgttgt ggagaagatc
      961 cttgatgcct tggttttgcg atcccacaag atccagccgg cggtggcgtt tgggcggcga
     1021 ttcgaccagg tgcctgctca aagctgggcg tggctggggc ggaacgtggc aggcccgatc
     1081 gtcttgatcc cggtgggttc ccacatcccg agcaccatat ttcggcgcgt gcggatccag
     1141 gtggagcggt tgattgatcc gcccgagatt tgggtgctgc cccccaagcg gatgtttgct
     1201 ccgcttggtc ggatggctgc ggtgctcaaa tccccggagt ttcaggagtt tatccgcgat
     1261 cgcgcggtgc cgctgttggg ccgaaagcgg cgaattcagt tgagccccgg cgtggccgca
     1321 atgcatccgc actccgccaa ccccgaatcg gatcattatc tcaggccgag gcaggagggt
     1381 gcggagatcg cccctcaaga gcggcgggag gaccttcgct ctg
//
LOCUS       DAOPTR010001074         1420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_71327_length_1420_cov_3.356777, whole genome shotgun sequence.
ACCESSION   DAOPTR010001074 DAOPTR010000000
VERSION     DAOPTR010001074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71327_length_1420_cov_3.356777"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="PKI05_15835"
     CDS             complement(<1..812)
                     /locus_tag="PKI05_15835"
                     /inference="COORDINATES: protein motif:HMM:NF040121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6513 domain-containing protein"
                     /protein_id="HOA53723.1"
                     /translation="MDYDDREVPSSCEHFLFVTGQLAEPLLNRMLPDLATAFEFQYSV
                     AVMPITIAGLMSVPWISERLKVPPKVTRILLPGRCQGDVGELSRRTGIPAERGPNDLH
                     ELPDFFKRRYRPCLPQDEYDLKIVSVLKDAANLEPSVVLDLADRLVAEGADAIALWHE
                     DREPFPNLGKLIKALRSHGHRVWIRSLEKTSYQAAAAEGADFLEPFRPDWAEVSAETW
                     AVVEPELPGTWQGVEEVIASLSEKHRRFLLKQPMTPVGLGLTQSILRCVEAR"
BASE COUNT          324 a          447 c          374 g          275 t
ORIGIN      
        1 tctcttgcct caacgcaccg aaggatgctc tgcgtcaatc ccagaccgac cggagtcatg
       61 ggctgcttaa gcaggaagcg gcgatgcttc tcgctcaaac tggcaatgac ctcctcgacc
      121 ccctgccacg tgccgggaag ttccggctca accacggccc acgtctccgc cgatacctcc
      181 gcccagtctg gacggaacgg ctctaaaaaa tctgctccct ctgccgctgc cgcttgatag
      241 ctcgtctttt ccagcgatcg gatccacacc cgatgcccat gggaacgcaa cgccttgatc
      301 aatttcccca gattcggaaa tggttcgcga tcctcatgcc acagtgcaat ggcatccgcc
      361 ccttctgcga ctaggcggtc cgccaaatcc agaaccaccg acggttccag attggcggca
      421 tccttgagga ccgacacaat cttgagatca tattcgtctt ggggcaaaca aggccgatag
      481 cgtcgcttga agaaatcggg caactcgtga agatcgttcg gacctcgctc cgctgggatg
      541 cccgtccgcc gagatagttc gcccacatcg ccttgacaac gaccgggcaa aagaatccgc
      601 gtcaccttcg gcggcacttt caaccgctcg ctgatccagg gaacgctcat cagcccagcg
      661 atcgtgatcg gcatgacggc caccgagtac tgaaactcga acgccgtcgc aaggtccggc
      721 aacattcggt tgagaagggg ctcggccaac tgacccgtca caaagaggaa atgttcacaa
      781 gacgaaggaa cttcacgatc gtcgtaatcc ataaacgacg cctccgcggc ctagccgctt
      841 ttgggcggga tgattggcgt tatattcgac aaaacagtta caggttgacc gacaacgctc
      901 tctggccacc aattcggcga ggggtgaccg ccaccgacgc cagtcagggc gatgttgtcc
      961 aaaaacatcc gtaccccggc agtgacccca cggtcacccc ggacttgggc ggcaagctcc
     1021 ggttgcctgg cctgtccgcc ggtgatgctt gtccgctact actctctatt ctacgcagcc
     1081 cccacggcga gacgtagata aattggggaa cctctgtaaa gatagctaaa cacaacccag
     1141 agacaagccc ccattcccca ccggtaaaat ccccgagccg ccggtggatg atggctcgaa
     1201 aattgcccac ggttaaggga caataaatct tcagcgtgga tttggttttg cgtggcaatc
     1261 cgcggcgggg atctcgtttt cggttgaagg ctgaaagagt agcatctcgc cacaagggcc
     1321 tatcccaaag ttccctctcc ccttgggaga gggttggggt gagggcaagc gaccgtcgga
     1381 gtcaattgag aaaaccggca gcgattcaag ccaccctcat
//
LOCUS       DAOPTR010001075         1420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_71333_length_1420_cov_3.049084, whole genome shotgun sequence.
ACCESSION   DAOPTR010001075 DAOPTR010000000
VERSION     DAOPTR010001075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71333_length_1420_cov_3.049084"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="PKI05_15840"
     CDS             complement(<1..620)
                     /locus_tag="PKI05_15840"
                     /inference="COORDINATES: protein motif:HMM:NF025664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53724.1"
                     /translation="MGTKDLIPPHGDLVEPLDLTVLAEAQADFLAHAKKLKKVPVSDA
                     DLSTVYRLGDGGLSPLTGPMDRETYDRVLDEEVIEHNGRLYAWTIPLALPITKALANQ
                     IGPGEEVALVNGRQEIVATLQVTDVFPWDKRKYLTRVYLTERFDHPGGDMVLKADGDK
                     DFLIGGKIQVLPQPKHPRFGKYVLRPKEVRALIREKGWERVVAFQT"
     gene            complement(817..1206)
                     /gene="rplU"
                     /locus_tag="PKI05_15845"
     CDS             complement(817..1206)
                     /gene="rplU"
                     /locus_tag="PKI05_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOA53725.1"
                     /translation="MYAIIQDGGRQYKVQVGEVIDVDYRLEKNKTADPAEFTPKEIRF
                     DRVLAIRDGSKFILGQPTISSAWVVADVIEPILGPKVYIQKFRRRKNYRRRKGHRQLY
                     LRVKIREIHTGQESGSTQAPAVEPAVV"
BASE COUNT          317 a          448 c          345 g          310 t
ORIGIN      
        1 ctcgtttgaa acgccactac ccgctcccat cccttttccc gaatgagcgc ccgcacctct
       61 ttcggacgga gaacatattt cccaaaccgc ggatgcttcg gctgcggcaa gacctggatt
      121 tttccaccga tgagaaaatc cttatcccca tcagccttaa ggaccatatc accgccggga
      181 tggtcaaacc gttccgtgag atagacccgc gtcaaatact tccgcttatc ccaggggaag
      241 acatcggtca cttgcaacgt ggcgacaatc tcttgccttc cgttaacaag cgccacctcc
      301 tcaccaggtc caatttgatt ggccaaggcc tttgtaatgg gaagtgccaa ggggatcgtc
      361 caggcataca atcgcccatt gtgttcgatc acctcctcgt ctaacacccg atcgtacgtc
      421 tcgcggtcca tgggaccggt caaaggactc aatcccccat cgcccaggcg ataaaccgtg
      481 gacaaatcgg catcagaaac gggcaccttc ttgagcttct tggcgtgggc caggaaatcg
      541 gcctgggcct ccgccaggac ggtcagatcg agcggctcaa ccaagtcacc atgaggagga
      601 attaagtctt tcgttcccat attcgcaatt gtgactgaaa tattactcag aaaaaaccgt
      661 caatctcgtc ccaatcaccc cgtcttatcc cgagtcacgc aatcatcccg cccgggcacc
      721 ccaacaatcc ccattcttca ggccgcacct cccagaaggt cgggcaccag tctgccccag
      781 tggtgggcga tttctgcaga tttgaaaagg cgggcttcag acgacagcgg gttcgacggc
      841 cggggcctgg gtcgaaccgc tttcctggcc tgtgtggatt tcccgaatct tgacccgcag
      901 gtaaagctgt cggtggccct tccgccgacg gtagttctta cggcggcgaa acttctggat
      961 gtaaaccttg ggaccaagga tcggctcgat gacatcggcc acaacccagg ccgagcttat
     1021 ggtgggctgg ccaagaataa acttcgaacc gtcgcggatg gccagcacac gatcaaagcg
     1081 gatttccttg ggcgtgaact ccgccgggtc ggccgtcttg ttcttttcta accgataatc
     1141 aacgtcgata acctcgccca cctgcacctt gtactgtcgc ccaccatctt gaatgatcgc
     1201 atacatgggt cgaatcctcg cctggaaaaa attgtctcaa acttcgttcg cgctgatttg
     1261 ccctattttt gggggccctt attgccacca ttagtgcatc tactactccg gcaccacacg
     1321 accgcgctag gcgcaatgcc gatcgactcg ccccacgcga gaacggtaaa ccctcattaa
     1381 acccggcaac tgccgaacgt tcaagggtca cggttggctg
//
LOCUS       DAOPTR010001076         1419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_71427_length_1419_cov_2.487537, whole genome shotgun sequence.
ACCESSION   DAOPTR010001076 DAOPTR010000000
VERSION     DAOPTR010001076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71427_length_1419_cov_2.487537"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="PKI05_15850"
     CDS             complement(<1..796)
                     /locus_tag="PKI05_15850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA53726.1"
                     /translation="MITLHHFTKTYGRTIAVNDLSMEVPLGEILGFIGRNGAGKSTTI
                     RFLATLIKPTAGTGTVAGFDVVRQPIEARHHLGYMPDMFGDYPGFRVRTFLEFFATGY
                     GLVGKLRRHRVSDVIERLGLDDYVSRPVRRLSRGMKQRLYLARCLLHDPPVLILDEPM
                     NGLDPEARMEFRNFLRQLAASGKTILLSSHILGELADTATSVAIIHRGNLVAHGGVSE
                     VVRDYTTGTRVVVRFLQPQRLSQEAIEALHPHVKLLSVDSSEALLQV"
BASE COUNT          342 a          370 c          367 g          340 t
ORIGIN      
        1 gaacttgcaa caaggcctca gaagaatcga ccgacaaaag cttcacatga ggatggaggg
       61 cctcgatggc ctcttgggat aatcgttgtg gctggagaaa tcgaaccacg acccgagttc
      121 ccgttgtgta atcccgaaca acctccgaca cgcctccatg agcgacgagg tttccccgat
      181 gaataatagc aacgctcgtg gcagtatcgg caagctctcc caaaatgtga ctcgacagga
      241 ggatcgtctt tcccgatgcc gccaattgac ggagaaagtt cctaaattcc atcctcgcct
      301 ccggatcgag gccattcatt ggctcatcca ggatcaaaac ggggggatcg tggagaagac
      361 accttgccag gtaaagacgt tgtttcatcc cacgagaaag cctccgaact ggccgggaga
      421 cgtaatcatc taacccaagt cgttcgatga cgtcgcttac acggtgtcgc cgtaattttc
      481 caaccaaacc gtaacctgtc gcaaagaact caaggaaagt acggacgcga aagccaggat
      541 agtcgccgaa catgtccggc atgtaaccga ggtggtggcg ggcctcaatg ggctggcgaa
      601 ccacgtcgaa accggcaacc gtgcccgtcc ctgccgtcgg tttgatcagt gttgccaaga
      661 aacgaatggt tgtcgatttg cccgccccat ttcggccgat gaagccaagg atttcgccaa
      721 ggggaacctc catgctgagg tcattgacag caatggttcg gccgtatgtt ttggtgaagt
      781 ggtgtagtgt gatcatcgtg ctcgtgggca attaccggtg ccgagggccg cacccccgat
      841 gtcaaaaatg tcgcatcctt ttcaagccac tctgaatttt aacaacccgg ttctgacaca
      901 aaacccttgg attgccccac cggctctagg agaaaacctg ttgattaggc aaaatacgcc
      961 gcctgtgctt gtgggggtgt tccgcgtaaa ataaatgagg tttggttgct gtacgacttt
     1021 tgcccgagtt tgtggggcaa tcgtccgaaa taatgtaagg cttgcccgag gaacttgctt
     1081 ttttgctatg catggttaac ataaatgcgg cagggttagc ttcagggcat ggcaaggaat
     1141 tcccataata tggataaacc ttcaccatcc ttccgctgtc gcctaatctg cctgtgttgc
     1201 tgtccggtct agggccggac agtacatctt acgatccttc ttgccgacat cgagctcgct
     1261 ttcaagtttt ttggcattgt agttttcttg acagttttga aaaagtgttc ggtcttgccc
     1321 agtgtgttga tgcaccaaac ccgtacttca aaccgaggat gagacatggc ttctgacttt
     1381 cgactcaagg aaaggctccc ttatatcacc gaccgactg
//
LOCUS       DAOPTR010001077         1417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_71568_length_1417_cov_2.732012, whole genome shotgun sequence.
ACCESSION   DAOPTR010001077 DAOPTR010000000
VERSION     DAOPTR010001077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71568_length_1417_cov_2.732012"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PKI05_15855"
     CDS             complement(<1..468)
                     /locus_tag="PKI05_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOA53727.1"
                     /translation="MTLIPFVIERTGREERAMDIYSRLLKDRIVFIGAPITDELANSV
                     VAQLLFLQSEDPKADVHVYINSPGGSVTAGLAIYDTMQFIRCDVATYCIGQAASMAAV
                     LLAAGTKGKRYALPNSRIMIHQPMAGVEGSATEIMIHAKEFKNLKQRINQILIK"
     gene            complement(607..1242)
                     /locus_tag="PKI05_15860"
     CDS             complement(607..1242)
                     /locus_tag="PKI05_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009101516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOA53728.1"
                     /translation="MFLDQWIALQGTAKASYRDYQRQRQMTLGDLLLENRIILLQGVI
                     HDGNASEVIMKLLYLQSENRRKDIHFYINSPGGSVTATLAIYDTMQILSCPVATYCVG
                     LAASGAAVLLAGGTKGKRFALPHSKVMIHQPYGQVGGQVTDIEIQTQEILRTRDILNR
                     ILSEHTGQPIERIAQDTDRDFYMTAEEAKNYGLVDDILTKQSQEKAEEEIE"
BASE COUNT          288 a          382 c          378 g          369 t
ORIGIN      
        1 tttgataaga atctgattga tccgctgttt aagattcttg aactccttgg cgtggatcat
       61 gatctctgtg gcgcttccct ccacacctgc catgggctgg tggatcatga tccgtgaatt
      121 gggcagggcg tagcgtttgc ccttggtgcc cgccgccagt aagaccgccg ccatggaagc
      181 cgcttgccca atgcagtagg tggctacatc gcagcgaatg aattgcatcg tgtcgtaaat
      241 cgccaagcct gccgtgacgc ttccgcccgg tgagttgata taaacatgca catccgcttt
      301 cgggtcttcc gattgaagga agaggagttg cgccaccact gaattggcca actcgtctgt
      361 gatgggtgcc ccaatgaaca cgatacggtc tttgagcagg cgactgtaaa tatccatcgc
      421 ccgctcttct cggcccgtac gttcaatgac aaacggtatg agtgtcatga tatgtctcct
      481 caatcactga cattgtttcc ttaggatcaa tggtctcttt cggggccggt cttcggctag
      541 gtctcatcgc tcgatcaacg agcctcaagg acgttcccat ccccacggag ggactccctc
      601 aaggactcac tcaatctctt cttctgcttt ctcctgggat tgcttggtca ggatgtcatc
      661 cacaagacca taattcttgg cctcctcggc cgtcatgtag aaatcgcggt cggtgtcctg
      721 ggcaatccgt tcgatgggct gccctgtgtg ttcggaaagg atacggttga gaatatcccg
      781 cgtgcggagg atttcctgag tctggatctc gatgtcggtc acctgcccac cgacctggcc
      841 gtaaggctga tggatcatca ctttggaatg cggaagggcg aagcgttttc ccttcgtgcc
      901 accggcaagg agcacggctg caccgctggc cgctaagccc acgcagtaag ttgccaccgg
      961 gcagctcaaa atctgcatcg tgtcatagat cgccagcgta gcggtaacac ttcccccagg
     1021 cgaattgatg taaaaatgga tgtctttccg ccgattttca ctttggagat acaaaagttt
     1081 catgatcact tcgctggcgt ttccatcgtg gataaccccc tggaggagga tgattcggtt
     1141 ctccagaagg aggtctccca gcgtcatttg gcgttgccgt tggtagtcac ggtaacttgc
     1201 ttttgcggtc ccttgcagag cgatccattg atccaggaac ataatcacgt ccttcttttt
     1261 cagaaatttc catctatcga tagggacgat ccgagcgtcc gttgggtgca tccgttgagg
     1321 cacaattacc cggccggacg tctagttgtg acttgagtac ggcggatcgc cggaaaaatg
     1381 ttatcgtacc ggttggccta ttggtctggc aagctgt
//
LOCUS       DAOPTR010001078         1412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_71910_length_1412_cov_4.191599, whole genome shotgun sequence.
ACCESSION   DAOPTR010001078 DAOPTR010000000
VERSION     DAOPTR010001078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71910_length_1412_cov_4.191599"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PKI05_15865"
     CDS             complement(<1..840)
                     /locus_tag="PKI05_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007332527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HOA53729.1"
                     /translation="MFEKLQSGLFEAIRTLTGKGKLTEANMREGLRMVQQALLEADVS
                     LPVVKAFLEKVSQEALGARVLQSLRPTHQLLAIVYEQLVALMGPVDHELRLKPSGATI
                     MLCGLQGSGKTTTAAKLGRYLQMKGRKPLLVAADLQRPAAIEQLQVLGEKIGVPVYVE
                     KGATDPLPVCRNALRQMSALGADVAILDTAGRLHIDEALMQQLAIIDRQIQPDEVFLV
                     VDAMTGQDAVNSAKAFHEALKLNGVILTKLDGDARGGAALSVKYVTGVPIKFIGTGEG
                     TDAL"
     gene            complement(1208..>1412)
                     /locus_tag="PKI05_15870"
     CDS             complement(1208..>1412)
                     /locus_tag="PKI05_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53730.1"
                     /translation="AHLRQRGYTLVDIQMLTPVTESLGAVEIHRREYLRRLASALKQP
                     VTFGDILAVGPQQVLALDHRWRE"
BASE COUNT          278 a          421 c          423 g          290 t
ORIGIN      
        1 gagggcgtcc gtcccttcgc cggtcccaat gaacttgatc ggcacgcccg tcacgtactt
       61 gaccgagagg gccgcaccac cgcgggcatc gccgtcaagc ttggtcaaaa tgaccccgtt
      121 gagcttgagt gcctcatgga acgccttggc gctgttgacg gcgtcctggc ctgtcatcgc
      181 gtccaccacc aggaacacct cgtcgggctg gatctggcgg tcgatgatgg ccaactgttg
      241 catgagggcc tcgtcgatgt gaagacgacc agccgtgtcg aggatcgcca catcggcccc
      301 cagtgccgac atttgacgca gcgcatttcg gcaaaccggg aggggatcag tagcaccttt
      361 ttccacatag accgggacac cgatcttttc cccaagtact tgaagctgct cgatggcggc
      421 gggtctttga aggtcggctg ccaccaaaag gggtttgcgg cccttcatct gaaggtatcg
      481 gcccaacttg gcggccgtcg tcgttttacc tgatccctga agcccgcaga gcatgatcgt
      541 ggccccgctg ggcttgagcc gcagctcgtg atccaccggg cccatcaggg cgacaagctg
      601 ttcataaaca atcgccagga gttggtgcgt tggacggagc gactgaagga cccgcgcgcc
      661 gagggcctct tgggagacct tctccaggaa ggctttgacc acaggcagac tgacatccgc
      721 ctcgaggagg gcctgctgga ccatccgcag cccctcccgc atgttcgcct cggtcaattt
      781 ccccttgccg gtaagtgtgc ggatggcctc gaagagtcct gattgaagtt tctcaaacat
      841 ggagtgttac ctgaaccgga gaacaaaacc gagcttgtac gcctgttacc gcgtcgctcc
      901 gccccaacgt tctacgagcg tcttggaaac ctaagaagtg gcgctgccat cgggcgaaaa
      961 ccttctgtga ccgcctgcgg catcatttca ccatcttagt cgtccccacc gtgttttggc
     1021 aatgcaaacg gcgtcgtttg agcgggtccc agccatgcga ttatggaaat ggtctgccgg
     1081 tttgctggga caagccggtc gcctaatgtg gagaaaagcg cgacatcgcg tccattgaga
     1141 gaatatagcg tcgtattcca cgacgacaag ccggcgcgca ctgccgggtc aatagattcg
     1201 gccaggttta ttcacgccac cggtggtcaa gggctaacac ctgttggggg cctacagcca
     1261 ggatatcgcc aaaggttaca ggctgtttga gggcggaagc gagtcggcgg agatattccc
     1321 gtctgtggat ctccacggcg cccagcgatt ccgtgaccgg ggtcaacatt tggatgtcca
     1381 ccaaggtgta accccgctgt cggaggtggg cg
//
LOCUS       DAOPTR010001079         1411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_72025_length_1411_cov_2.004425, whole genome shotgun sequence.
ACCESSION   DAOPTR010001079 DAOPTR010000000
VERSION     DAOPTR010001079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72025_length_1411_cov_2.004425"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PKI05_15875"
     CDS             <1..433
                     /locus_tag="PKI05_15875"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HOA53731.1"
                     /translation="QCRVHLERTIPPQAFWPRPKVFSAIIHIESDAQRRSRIEDLAFF
                     HAWIRRLFLHRRKFLRSQVLSALGRDASKPDVDAMLQELGLDSQLRAEQLSISQIIEL
                     AEAARKRFPTLVDSIRGSSSEADEDAASSEGVKEDQRDSED"
     gene            514..768
                     /locus_tag="PKI05_15880"
     CDS             514..768
                     /locus_tag="PKI05_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53732.1"
                     /translation="MYEDTSYPKTQFIEMLGRQLLRVVTEGGPTPAELAKVGLKEDSI
                     VRVNRQGDIELRRPDRWDVIGGLIGDFEARIKQETGLDWV"
     gene            complement(737..>1411)
                     /locus_tag="PKI05_15885"
     CDS             complement(737..>1411)
                     /locus_tag="PKI05_15885"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOA53733.1"
                     /translation="WQVIHSRRRPRPEEEPTTIYRASQTSEHETLYAKRAVLRRVLRR
                     AIDMRQATELSVAKLMSENVIGVHPKTPIYKVVQTIKEKCVSHVLVVDRKGLLLGIIS
                     HRDLSRRTGRYAEDIMTRSPVTVHVSTPATQAISLMLEKDISCLPVVEDGHVRGVLTK
                     TDVMIGFQALIQALELVLTEPAEEEELDSFTENDVAPDESPGDTPAAIPTEISRITPN
                     PNPSLA"
BASE COUNT          313 a          343 c          418 g          337 t
ORIGIN      
        1 tcaatgtcgc gtacatttgg aacggaccat cccaccccag gcgttttggc cccgaccgaa
       61 ggtattttcg gctatcatcc acatcgaaag cgacgcccaa cgtcgcagcc gcattgaaga
      121 cctggccttt tttcatgcgt ggattcgcag gttatttctc catcgccgga agttcctgcg
      181 aagccaagtc ttgagtgccc ttgggcgaga tgcaagtaag ccagatgtgg acgcgatgct
      241 acaggaactt ggccttgatt cgcaattgcg ggctgagcag ttgagcattt cccaaatcat
      301 tgagttggcg gaagcggctc ggaaacgatt tcccacattg gttgattcga tccggggaag
      361 tagttcggag gccgatgaag acgcggcgtc gtcagaaggc gtgaaagaag accagcgaga
      421 tagcgaagat tgactggcga gggcagggta ggttatcttg tttcaaaatg gaggtgtcgt
      481 gtcgatcagg tcatcctgtc aagtaggcaa actatgtacg aggatacttc ttaccccaag
      541 acccaattca ttgagatgtt gggccgacag cttctccgtg tcgtgacgga aggcggtccc
      601 acaccggcag agctggcaaa ggttgggctc aaagaggaca gcatcgttcg agtgaatcgg
      661 cagggcgata ttgaattgcg tcgtcccgat cgatgggacg tcattggtgg gctgatcggt
      721 gactttgaag cccgcatcaa gcaagagacg ggtttggatt gggtgtgatc ctcgagattt
      781 ctgtcgggat ggctgcgggg gtatcgccgg gcgattcgtc cggggcaacg tcgttttctg
      841 tgaaactgtc caactcctcc tcttcggcag gttctgtgag aacaagctcc aaggcctgaa
      901 tgagggcttg aaatccgatc ataacatcgg ttttggtgag gacgccccgg acgtggccat
      961 cttccacaac aggcaaacaa gaaatgtctt tttccaacat cagcgaaata gcctgagttg
     1021 ccggggttga gacgtgcacg gttaccggcg atcgcgtcat gatgtcttct gcgtagcgac
     1081 cggttcgtcg ggatagatca cgatggctaa tgatgcccag gagtaatccc tttcgatcaa
     1141 caacaaggac atggctaacg catttttcct tgattgtctg gacgacctta taaatcgggg
     1201 tctttgggtg aactccgatg acattctctg acatcagttt ggccacggat agctcggtgg
     1261 cttgacgcat gtcaatggca cgccttaaca cacgccgaag gacagcacgt ttggcgtaaa
     1321 gagtctcgtg ttccgacgtc tgcgacgccc gatagatggt tgtgggctct tcctcggggc
     1381 gaggccgtcg tctgctgtgg ataacttgcc a
//
LOCUS       DAOPTR010001080         1407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_72280_length_1407_cov_3.369822, whole genome shotgun sequence.
ACCESSION   DAOPTR010001080 DAOPTR010000000
VERSION     DAOPTR010001080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72280_length_1407_cov_3.369822"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..387
                     /locus_tag="PKI05_15890"
     CDS             205..387
                     /locus_tag="PKI05_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53734.1"
                     /translation="MAFLLDEPPNEVFQVNGTTPAFPAVQSYYVAPAPTLVRGYALLP
                     RAKEKVLGNNVPPAHR"
     gene            complement(393..>1407)
                     /locus_tag="PKI05_15895"
     CDS             complement(393..>1407)
                     /locus_tag="PKI05_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53735.1"
                     /translation="ARLTPLAKENYEKLLTRLGPPDRQQLAPIAGDLGRFEIVLSGGR
                     LFGGLYDVVPPIDIVDGRIVPTGRVRDILIGYLGTTGELGWIGKLNRTFSTRPDPNGY
                     SRGLLGLWRREMPGVTVFSFQPEVLAAVTEQLHWIEAPEEAHIRVELKDLSHAQITPL
                     LNRFVYWRTQQTSLGNLLFLRDLEQQLHVPGEECKNVAEIIFDAQLQCPLGGEYVFQP
                     DETGIPRWTSTAFLPTVSPTGSGIQLPFLQFGNQPTSSIPGPVDPIAEREKAMGSAPE
                     NVARPDQREEPKASQRPAKSSQEFLAPPLNWFRGLSGAAVVKPEAVTAHLEILMEWPA
                     EKE"
BASE COUNT          304 a          395 c          387 g          321 t
ORIGIN      
        1 cctattgctt gcgacaattt gcctattgta atcgcgcgct cgaaatattt cacctttcaa
       61 taaaagtccg cgtcgatttc gcttgcattt ggagaggtgt tagcttcgct catccatcac
      121 tggattggaa gcgaggcgac ttaggtcgga gctagtctcc gacccgcggg cctaccaaaa
      181 atgggaagcg caaagagctt ttttgtggcc tttctcctcg atgaaccccc gaatgaggtc
      241 ttccaagtca atggcacaac tcccgcattt cctgcggtcc agtcgtatta cgtggccccg
      301 gcacccactt tagtgcgcgg ttacgctctc ttaccacgtg ctaaagagaa agttttgggt
      361 aataacgttc caccagccca tcggtaaacg ggtcactctt tttctgccgg ccactccatc
      421 agaatttcca gatgggccgt gactgcttct ggcttgacca cggcagcgcc agaaaggcca
      481 cgaaaccagt tcaaaggtgg agccaggaac tcctgcgacg acttggcggg cctctgtgag
      541 gcctttggtt cttctctttg atcggggcgg gcgacatttt ccggggcgct ccccatggcc
      601 ttctcacgct cggcgatggg atctaccggt cccggaatag aggatgttgg ttggttgccg
      661 aactgcaaaa acggtaattg aataccgctg cctgtgggtg atacagttgg aagaaacgcc
      721 gtagaagtcc accgcgggat accggtctca tctggttgga aaacgtattc tccccccaaa
      781 gggcattgta actgtgcgtc aaaaatgatt tctgcgacat tcttacactc ttcaccgggg
      841 acatggagtt gctgttccaa gtcccgcaaa aagagaaggt tgcccagcga tgtctgttgg
      901 gttcgccagt aaacaaagcg attgagcaag ggtgttatct gggcatgcga caagtccttt
      961 agctccacgc ggatatgggc ctcctcgggc gcctcgatcc aatggagttg ctcggtcacc
     1021 gcagccaaga cctccggctg gaaggaaaaa accgtcacgc cgggcatctc acggcgccat
     1081 agtcccaaaa gaccgcgact gtacccgttt gggtcggggc gtgtgctaaa cgtgcggttc
     1141 agtttgccga tccaacctag ctcgccggtt gtgccgagat agccgatcaa aatgtcacgc
     1201 acacgtcctg tggggacaat gcggccatcc acgatgtcga tgggcgggac gacatcgtac
     1261 aaccctccga acaaccgtcc gccactgagg acaatctcaa agcgtcccaa atcccccgcg
     1321 ataggcgcca gttgctgtct gtcgggcgga ccgagccggg taagaagttt ctcgtagttc
     1381 tccttggcaa gcggggtgag tcgcgcg
//
LOCUS       DAOPTR010001081         1404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_72491_length_1404_cov_3.540400, whole genome shotgun sequence.
ACCESSION   DAOPTR010001081 DAOPTR010000000
VERSION     DAOPTR010001081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72491_length_1404_cov_3.540400"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..442
                     /locus_tag="PKI05_15900"
                     /pseudo
     CDS             41..442
                     /locus_tag="PKI05_15900"
                     /inference="COORDINATES: protein motif:HMM:NF014793.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
     gene            543..>1404
                     /locus_tag="PKI05_15905"
     CDS             543..>1404
                     /locus_tag="PKI05_15905"
                     /inference="COORDINATES: protein motif:HMM:NF014065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HOA53736.1"
                     /translation="MAVRVPKGYLVAGVHCGLKRDLRKPDLSLIVSEVPATAAGVYTQ
                     NIVCAAPVQLDRRRTPSNRIRVVVINSGNANACTGERGLRDAEEMARLAGKAVGAEED
                     QVLVMSTGVIGVYLPMEKIAHGIELAAARLGNDEEALIMAARGMMTTDTVYKLSGRTL
                     SLARREIQITGMVKGAAMMGPNLATMLGVIFTDATLEVDTAQQLLREVVDDTFNCINV
                     DGHTSTNDTVLFLANGAAGGPPLSGDLLDDFKQAFHSLCTELARMIPADGEGATHLIT
                     IEVRGCRTRAD"
BASE COUNT          293 a          391 c          421 g          299 t
ORIGIN      
        1 gacgacccat ttcccggagt gaaacgaaag tctttccgcg tacaacatcg gtcggcatcg
       61 ccatacgccg gagatcgagc agatcctttc cacggtggcg ggggcggagg tgcaaatcgt
      121 ctttaccccg catctggttc cgatggatcg cggtatcttg acaacgactt attcccatcc
      181 gacgcgacag gtcaccgagg acgaagtcct ggattgccta cggtcgttct acgatgacga
      241 accttttgtg cgggtagtgg atcaccttcc ggccactaag gacacatcgg ggacgaattt
      301 ctgtcatatt acggcacgga ttgtccgcga ccggatcatc accgtcagtt gcctcgacaa
      361 tctgatcaag ggggcgtccg gggccgcggt gcagaacttc aacctaatgt atggttttcc
      421 cgagacaacg gccctgctat gacaatagag gccgacacgg cgggctaaac cgccttttga
      481 gatcggacgt ctcgattgtt aacaggctag cgacggtttc gcagtagaag aaggccttgc
      541 ccatggcagt acgtgttcct aagggttatc ttgttgccgg tgttcattgt ggattgaagc
      601 gcgatttgcg caagccggat ttatcgctga ttgtttctga agtcccggcc acggccgccg
      661 gtgtttacac gcagaacatc gtttgcgcgg caccggtcca actggatcgc cgacggaccc
      721 cgagcaaccg catccgcgtt gtcgtcatca attcgggcaa tgccaacgct tgtacaggag
      781 aacgtggcct ccgtgacgcc gaggaaatgg cacggcttgc tggtaaggcc gtgggcgcag
      841 aggaagatca ggtcttggtg atgtccacgg gcgtgatcgg cgtatatttg ccgatggaaa
      901 agattgccca cgggatcgaa ctggcggcgg cccgattggg caacgacgaa gaggccctca
      961 taatggcagc ccgcggcatg atgaccaccg acacggtcta taagctttcc gggcggacgc
     1021 tttcccttgc gcggcgagaa atccagatca cgggcatggt caagggtgcg gccatgatgg
     1081 ggcccaacct ggccaccatg cttggggtca tcttcaccga tgccacactg gaggtggata
     1141 ccgcgcaaca gttgctccgc gaggtggtgg atgacacctt caactgcatt aatgtcgatg
     1201 ggcacactag caccaatgac acggtattgt ttcttgccaa tggtgcggcg gggggaccac
     1261 ccctgtccgg cgatttgcta gacgatttca agcaggcttt ccacagtctg tgcactgaat
     1321 tggctcgcat gatcccggca gatggcgaag gggccacgca cctcatcacc atcgaagtcc
     1381 gcggctgtcg tacccgtgcg gatg
//
LOCUS       DAOPTR010001082         1401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_72709_length_1401_cov_3.216196, whole genome shotgun sequence.
ACCESSION   DAOPTR010001082 DAOPTR010000000
VERSION     DAOPTR010001082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72709_length_1401_cov_3.216196"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..>1401
                     /locus_tag="PKI05_15910"
     CDS             43..>1401
                     /locus_tag="PKI05_15910"
                     /inference="COORDINATES: protein motif:HMM:NF023218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOA53737.1"
                     /translation="MGNIFLTPERGFDITLRAFNGSTDPQCLEGNLRILDYKDRPIWQ
                     KDVRLDIPQQSMAEERFSQLLAGRRGFFRVTWEPQGAPPQSLRIANIDPVIEQDTAFG
                     MNHAFPWDFLLDLSHAAGLRWWRDWSCQWRLVQKTEGGPFDFQIPDTQIKRVLQAGGN
                     PLVLLPFPATEWSAIVDTDRVAREAGSNSYLQRRLIVAQKPRDVVEFGQYVRATVEHY
                     HKSVKVIEILNEPLFTSYALPNSFGWNISDYLDILKTAYESAKAVDPECLVIGGIAAP
                     PESNWVRQFIEQGGLQWCDVMNLHLYPHRGSPDAYESSFAACHRLMEARGQVRPIWVT
                     EIGCYADDDPPTLPFSVGDEAMNRSLRPSEYRAAIDLVKFAAVMGAAGVKKVFYHAGT
                     CGALNENTAGNTFFEYGGEPRKMYAAQAVLSDFLGADWNFVGKWGQDQMLQGFRFQSK
                     GRE"
BASE COUNT          307 a          402 c          392 g          300 t
ORIGIN      
        1 cgacctgctc gcagcgtaga gtcagcgatt tccaccgatc ggatgggtaa cattttcctc
       61 acgccagagc gtggcttcga cataaccttg cgagcgttta atggatcaac ggatccgcaa
      121 tgcctggagg gcaaccttcg catcctcgat tacaaagacc ggcccatttg gcaaaaggat
      181 gtacgattgg acatcccgca gcaaagcatg gccgaagaac gtttttccca acttctcgca
      241 ggtcgccgag gattcttccg cgttacctgg gaaccccaag gggccccgcc ccaatcgcta
      301 cggatagcca atatcgaccc cgtcatcgag caagataccg cgtttggtat gaatcacgct
      361 ttcccttggg actttctact agatctctcc catgccgccg ggttgcgctg gtggcgggat
      421 tggtcttgtc agtggcgctt ggtgcagaaa accgaaggtg gccccttcga tttccagatc
      481 cccgataccc aaatcaagag ggtcctccaa gcgggcggca atcctctggt gctcctaccc
      541 tttccggcta cggagtggtc ggcaatcgtg gacaccgacc gtgtcgcgcg ggaggccggc
      601 tccaatagtt acctccaacg gcgtcttatt gtggcacaaa aaccaaggga cgtggtggag
      661 ttcggccagt acgtccgggc aacggttgag cactaccata agagcgtcaa ggtcattgaa
      721 atcctcaatg aaccgctttt cacgagctat gccctgccta actcctttgg ttggaacatt
      781 tctgactatc tcgacatact caagacggcc tacgagtcag caaaagcagt agatcccgag
      841 tgccttgtca tcgggggaat cgctgcaccg ccggaatcga attgggtaag acagttcatc
      901 gaacaaggcg ggttacagtg gtgtgacgtg atgaatctcc atctgtatcc ccaccgagga
      961 agtcccgatg cctacgagtc ctccttcgct gcgtgtcacc gactcatgga agcccgcgga
     1021 caggtgcggc ccatttgggt cacggaaatc ggctgttacg ccgatgacga tccccctacc
     1081 ctgccgttca gcgtggggga cgaggccatg aaccgcagct tacgaccgag cgaataccga
     1141 gccgccatcg accttgtcaa gttcgcggcg gttatgggtg cggccggggt caagaaagtc
     1201 ttctaccatg cgggaacctg tggcgccctc aatgagaata ccgcgggcaa cactttcttt
     1261 gagtacgggg gtgaaccccg aaagatgtac gcggcccagg cggttctctc cgattttttg
     1321 ggagccgact ggaactttgt cggcaagtgg ggccaagatc aaatgcttca agggttccgt
     1381 ttccagtcaa aagggcggga a
//
LOCUS       DAOPTR010001083         1400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_72791_length_1400_cov_3.271375, whole genome shotgun sequence.
ACCESSION   DAOPTR010001083 DAOPTR010000000
VERSION     DAOPTR010001083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72791_length_1400_cov_3.271375"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..1015)
                     /locus_tag="PKI05_15915"
     CDS             complement(74..1015)
                     /locus_tag="PKI05_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOA53738.1"
                     /translation="MDQDYSELLQRLRTNINGVLLGKESVVEMCLVALLAGEHILLED
                     VPGVGKTLLGKALAKSIAGEFRRIQFTPDLLPSDITGSNLYNAERREFVFYPGPIFAN
                     IVLADEINRATPRTQSALLEAMNESQVTAEGTTFPLPQPFLVIATQNPAEFEGTYPLP
                     ESQMDRFLLRVSIGYPSREYERQLLAQHRDGEPVDRLEPVLSCEQVLALQAATRQVYV
                     EDSVADYLLNLVHATRECPEIRVGASVRASLALYRAAQAKALLDGRDYVVPDDVQSLA
                     VPVLAHRVITHGYLHGEHREAVEALIHRLVEDTPSPT"
BASE COUNT          286 a          404 c          419 g          291 t
ORIGIN      
        1 ggcagcgatt cgagccaccc tcacccccga cccctctccc accgaacggg cgaggaagat
       61 tggggatagc cttttaggtt ggcgacggcg tatcctccac caggcggtgg ataagggcct
      121 cgacggcctc ccgatgttct ccgtgcaaat agccatgggt gatcacgcgg tgggcaagca
      181 ccggaacggc caggctttgg acgtcgtccg gcactacgta gtctcgacca tccaagaggg
      241 ccttggcctg ggccgcccgg tacagggcga gtgatgcccg cacactggcc cccacgcgaa
      301 tctccggaca ttcccgtgtg gcgtggacca agttgagaag gtagtccgcc acactgtctt
      361 ccacatacac ttgccgcgta gctgcttgaa gggcgaggac ttgctcgcag gaaaggaccg
      421 gttccagtcg atccacgggt tcgccatccc ggtgctgcgc cagcagttgc cgctcgtact
      481 cgcgactagg gtaacctatt gaaacgcgga gcaagaagcg atccatctgg ctttccggca
      541 ggggatacgt cccctcgaat tcggcgggat tctgggtggc aatgaccaga aacggctgcg
      601 gcaggggaaa cgtcgtccct tccgccgtca cttgcgattc attcatggcc tcgagaaggg
      661 cgctttgcgt gcgcggcgtg gcacgattga tctcatccgc caagacaata ttggcgaaaa
      721 taggaccggg ataaaagaca aattctcgtc gctccgcgtt atataagttg ctccccgtaa
      781 tgtctgaggg gagaaggtcg ggcgtgaatt ggatgcggcg gaactccccg gcgatgctct
      841 tggcaagggc ctttcccaaa agggttttgc ccacaccggg gacatcctca agcaagatgt
      901 gttccccggc aagaagggcc accagacaca tttcgaccac agattccttc ccgaggagga
      961 ccccgttgat attggtccgc agtcgctgca aaagctcgga atagtcctgg tccatcgaat
     1021 gcgccctcgc cctcagccgt ctttggtaac cccgctcgga ggatcgtgtc accggaagat
     1081 cctcagtgtt tcgggtatct tgccaactcc actcagtgta cccatttcga caggtaactg
     1141 tcatggtagg tcattctttg tggggggata cgtgtgcctg cctcaattct gccgctgggg
     1201 cggcttgccc tacgcggcat gaaacttgcc aattgcgggt acggttcgta tcttttagcg
     1261 gtaggggctt catggtagcc ggttcatcgt gctgttaggg ggaaacggcc atgtcggtgt
     1321 ggaaagccat tagccggcgt tttatcggac aaccttttgg aaagaaggag gaaacgcaac
     1381 aaccgacaga cccccgagag
//
LOCUS       DAOPTR010001084         1396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_73074_length_1396_cov_3.248322, whole genome shotgun sequence.
ACCESSION   DAOPTR010001084 DAOPTR010000000
VERSION     DAOPTR010001084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73074_length_1396_cov_3.248322"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PKI05_15920"
     CDS             <1..286
                     /locus_tag="PKI05_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOA53739.1"
                     /translation="HPYTEASVVFFNQETGQGSSANIQPDGSFRLPERIEVGTYIVYL
                     APKMEEDPAAEPKPVSIDKTVPEKYWSESTSDIRVEVKTGSNQFNIDLKP"
     gene            942..>1396
                     /locus_tag="PKI05_15925"
     CDS             942..>1396
                     /locus_tag="PKI05_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53740.1"
                     /translation="MHDLMELVTFIVDHTPALWQAADFIIPENALQGYWVSARSRFER
                     WASTLKELTSRSSTASREYRFRNQLAGILEEILTNEILTRVWTAALSLYDQQRKVDWA
                     GPIGQSAFLAQLELRVRSLGILSDGHFLPGEDIQKLLQLCERNDRWCDLL"
BASE COUNT          310 a          374 c          381 g          331 t
ORIGIN      
        1 gcacccttat acggaggcct cggtcgtctt tttcaatcag gaaacaggcc aaggaagttc
       61 ggcgaatatc caacctgatg gttccttcag acttcccgaa aggatcgagg tcggcaccta
      121 catcgtgtat ctggcgccga agatggaaga ggaccccgcg gcggaaccga agccagtctc
      181 cattgataag acggtgccgg aaaagtactg gagcgaatca acgagcgaca tccgggttga
      241 ggtaaagacg ggcagcaatc agttcaatat tgacttgaaa ccttagcata gcggctcggc
      301 ctcggccggg acatcggttt ctgtttgcac ccgcccgcat ccatcgtgaa ggcatgtttc
      361 cttcctgagc gatgaatggg ggcggggttt ctcattatta gggtcagaga attcgggcac
      421 agggaaaacc cctaaaaggc ggttagagtc caccttttcg caaaatcttg ccgacaaatg
      481 tcgttttact cctttccgga aagctctcgc cgacacgtct ggcgatccaa gaagctttta
      541 aatttccttt atcagctctg acgtcttccc tttgcacgct tgcgatgaag agccggaccc
      601 tgtatcccac acctgtcggc taccaaaatc ggcggggtga ccatgatgtc ttttttcacc
      661 accttaggca cgcagaggtg ccgaaactcg tcaacgggat ataacctaat ggcgccttcc
      721 cgccggtatt gggtgtttcc cgggcagccg agagcctttc gtgctagccc acgaagccgc
      781 tcgttttgcc cccaagcgct aggtgtcgta ctgctttgag agtgggacct cgacaaatag
      841 gattgcgatg aggtgtatgg cattccattt gcacacgggg aggaatcgag caaaatgatc
      901 atccaaactg tcgacagagg gcccccgcgg tgggtgcgtt tatgcacgac ctgatggaac
      961 ttgtgacctt cattgttgat catacgcctg ccctttggca ggcagccgac ttcatcattc
     1021 ccgaaaacgc ccttcaaggc tactgggtca gtgcgaggag tcgtttcgaa cgttgggcct
     1081 cgacgctaaa ggaactcacg tcccgctcat cgacggcctc tcgggaatat cgttttcgga
     1141 accaattggc tggcattctg gaagagatcc tcacgaatga aattctcacg cgggtgtgga
     1201 cggctgctct ttccctttat gatcaacagc ggaaggtcga ttgggcaggg ccgatcggtc
     1261 aaagtgcttt tctcgctcag ctagaactgc gtgtccgttc gctggggatt ttaagcgatg
     1321 gccatttcct cccgggggag gatatccaga aactcctgca actctgtgaa cggaatgacc
     1381 gttggtgtga cctcct
//
LOCUS       DAOPTR010001085         1394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_73214_length_1394_cov_3.229276, whole genome shotgun sequence.
ACCESSION   DAOPTR010001085 DAOPTR010000000
VERSION     DAOPTR010001085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73214_length_1394_cov_3.229276"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="PKI05_15930"
     CDS             <1..875
                     /locus_tag="PKI05_15930"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOA53741.1"
                     /translation="LAEHKLLKRPCVIKLIRPDKAGDPRLIARFEREVRTMARLSHWN
                     TVEIFDYGISAEGVFYYVMEYLPGLDLGQVVAQFGRQHPARIIYILRQVCDALREAHS
                     LGFIHRDIKPANIFLAHRGGVSDVVKVLDFGLVKPISHRGESLDLTLDGAVTGSPLYM
                     SPEQVLGEDNLDARTDIYSLGAVAYYLLTGRPPFSGEKPIKVMLAHVYETVTPPEKVV
                     PDLPPDFCQVVLRCLEKDPDRRFANVDELDHALLSCADADGWDREKAAAWWRRHLPSD
                     AQPKLEVSQVSAPV"
     gene            1099..>1394
                     /locus_tag="PKI05_15935"
     CDS             1099..>1394
                     /locus_tag="PKI05_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008683576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOA53742.1"
                     /translation="MPNTPTGPKTLFEKIWDAHVVHTDEGGQSLLYIDLHLVHEVTSP
                     QAFEGLRLAGRKVRRPERTVATVDHNVPTTDRSLPIADPISKQQIDTLRRNCQE"
BASE COUNT          294 a          384 c          395 g          321 t
ORIGIN      
        1 acttggcaga gcataagctc ctcaagcggc catgcgtgat caaacttatc cgcccggata
       61 aggccggcga tccccgactc atcgcacgat ttgagcgcga agtgcggacg atggcacggt
      121 tgtcccactg gaataccgtc gagattttcg actacggtat cagtgcggaa ggggttttct
      181 actacgttat ggagtacctt ccgggtttgg atcttggaca agttgtggcg caattcggtc
      241 ggcagcatcc ggcacggatt atttatatct tgcggcaggt gtgcgatgcc cttcgcgagg
      301 ctcatagttt ggggtttatt caccgtgaca tcaaacctgc caacatcttt ttggcacacc
      361 gaggcggggt ctccgatgtg gtgaaggtcc tggattttgg attggtgaaa ccgatttccc
      421 atcgcggtga gtccctcgat ctaacactcg atggtgccgt gacgggctcg ccgctttata
      481 tgtcgccaga acaggttcta ggggaagaca atcttgatgc ccggacggat atctattccc
      541 taggggcggt ggcctactat ttgttgacag gtcggccacc gttttcggga gagaaaccga
      601 tcaaggtcat gttggcccat gtctatgaga ctgtcacgcc gccggagaaa gtggtgccgg
      661 acctgccgcc tgatttctgc caggtagtcc ttcgctgtct ggaaaaggat cccgatcggc
      721 gattcgcaaa cgtggatgaa ctcgaccatg ccctcctgag ctgtgctgac gcggacggtt
      781 gggatcgcga aaaggccgcc gcgtggtgga gacgtcatct gccaagcgat gcccagccca
      841 agctggaggt ttcgcaagtg tcggcccccg tttgagtgga ccagcgacct cccccactca
      901 tccgcgatta gtccccccag cgccaattga tccaatggcc agttgccctg tacgctcatg
      961 ggtgacgtcc ttcaaggggt ctgatggcct acgctagggg gaaacctaac caaatcgagc
     1021 acgtcagcgt aaaatataca atctttgtga cctcgcgaac tgtcacttga tgcataggct
     1081 tcatgtggat atcgcactat gccgaacaca ccgactggac cgaagacgct cttcgagaag
     1141 atttgggatg cccacgtcgt tcacaccgat gaggggggcc agtcccttct ctatattgac
     1201 ctccatcttg ttcacgaggt gaccagtccc caagcctttg aggggttacg gctggccggg
     1261 cggaaagttc ggcgtccgga gcggaccgtg gccaccgttg accataatgt gcccactaca
     1321 gataggtcac tccccatcgc ggaccccatc tcgaagcaac aaattgatac gcttcggcga
     1381 aattgtcaag aatt
//
LOCUS       DAOPTR010001086         1393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_73300_length_1393_cov_2.573244, whole genome shotgun sequence.
ACCESSION   DAOPTR010001086 DAOPTR010000000
VERSION     DAOPTR010001086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73300_length_1393_cov_2.573244"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PKI05_15940"
     CDS             <1..347
                     /locus_tag="PKI05_15940"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53743.1"
                     /translation="GYPLRFDLGSEWRKWTDRPFVFALWVARSGVTDLDSWHKVFSLA
                     RDRGLDDLVNIAQAASRDLGLSFDECHQYLSKNMHYRLGEDELESLTLFRSLAVKHGI
                     LAPSARLTFHGG"
     gene            337..>1393
                     /gene="mqnC"
                     /locus_tag="PKI05_15945"
     CDS             337..>1393
                     /gene="mqnC"
                     /locus_tag="PKI05_15945"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HOA53744.1"
                     /translation="MVVSILDRATSGQRLGDEEALFLFEHASLALLGEAADAVTRRLH
                     PENYRTYNIDRNINYTNICVSGCKFCAFYRPPGHPEGYVIDQPTIFQKIEETIELGGN
                     QILLQGGLNPSLPLSWYEELLQAVKQRFPQINIHGFSPPEIHFLAKREKITVEEVLRR
                     LKNAGLGSLPGGGAEILVDRVRKELKSHKVDTEGWLEVCRTWHRLGGRGSATMMFGHV
                     ETFSDRMAHLRRLRDLQDETGGFTAFICWTFQPKNTALSHLRKHGAYDYLRTLAISRL
                     YLDNFANIQASWVTQGAAVGQLSLRFGANDMGSLMIEENVVAAAGTRFRTSESELRQL
                     ITQAGYVPRRRNVFYELV"
BASE COUNT          310 a          378 c          386 g          319 t
ORIGIN      
        1 cgggctatcc cttgcggttc gacctggggt ccgaatggcg gaaatggacc gatcggccgt
       61 ttgtattcgc cctgtgggtg gcacggtccg gcgtcaccga tcttgactca tggcataaag
      121 tctttagcct tgcccgggat cgtggcctgg atgatttggt caacattgcc caagcggcat
      181 cgcgtgacct gggcttgtcc tttgacgagt gtcaccaata cttatcaaag aacatgcatt
      241 acaggcttgg ggaagacgag cttgaatctt tgactctctt tcgatcgtta gccgtaaaac
      301 atggaatcct tgcaccttca gccagactca cattccatgg tggttagtat cctagacaga
      361 gcgacctcgg ggcaaagact cggcgacgaa gaggcccttt ttctgttcga acatgcgagc
      421 ctagccctct tgggggaagc ggcagacgcc gtaacacgcc ggttgcatcc tgaaaactat
      481 cggacgtaca acatcgatcg gaacattaat tacaccaata tctgcgtttc cggatgcaag
      541 ttctgtgcgt tctaccgtcc cccgggccat cccgaaggct acgtaattga ccaaccgaca
      601 atcttccaaa aaatcgagga gacgatcgaa ctgggcggaa accagatcct tttacagggg
      661 gggcttaatc cttctttgcc gttaagctgg tacgaagagc tgcttcaagc tgtgaaacaa
      721 cgctttccgc aaatcaatat tcatggcttt agccctcccg agattcattt cctggccaaa
      781 cgcgaaaaaa tcactgtgga ggaggtcttg cgtcggctaa aaaatgcggg attgggaagc
      841 ctgcccggcg gaggtgccga aatcctcgtg gaccgtgtcc gcaaggagct aaaaagccat
      901 aaggtcgata cggaaggctg gctggaagtc tgtcgcacct ggcatcgcct gggggggcgg
      961 gggtccgcca cgatgatgtt cggacatgtg gagacattct ccgatcgaat ggcccatctc
     1021 cgacgcctcc gggatcttca ggatgagaca ggtgggttca ccgcttttat ctgttggacg
     1081 tttcagccca aaaataccgc cctcagccat ctgcgaaaac acggggccta tgattacctc
     1141 cggaccttgg ccatcagtcg gctttacctc gataactttg ccaacatcca ggcaagctgg
     1201 gtaacgcagg gggccgccgt gggacagttg tcgctccgct tcggggccaa tgatatgggt
     1261 agcctgatga tcgaggaaaa cgtggtagct gcagcaggga cccgttttcg cacctccgag
     1321 tccgagcttc gccagttgat cacccaggcc ggttatgtgc cccgccgccg aaacgttttt
     1381 tatgagcttg tcg
//
LOCUS       DAOPTR010001087         1392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_73353_length_1392_cov_3.186238, whole genome shotgun sequence.
ACCESSION   DAOPTR010001087 DAOPTR010000000
VERSION     DAOPTR010001087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73353_length_1392_cov_3.186238"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(297..>1392)
                     /locus_tag="PKI05_15950"
     CDS             complement(297..>1392)
                     /locus_tag="PKI05_15950"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOA53745.1"
                     /translation="KRLKTRTQLCCTNEDVIRLVTEGILKAIENDSEAFVFSVSQNDW
                     YNYCECENCQALAKAEESQIAPVLQMVNRVAEAVEKRYPDKAIETLAYQWTRKPPKTM
                     RPRPNVIIRLCSIECCFSHPLATCDLPANRAFVEDLQGWAKIGNRLWVWDYVTDFRHY
                     YLPFPNQRVRNDNIKLFVANNVKGIFEQDTYNTPNSELAALGGYMTAKFLWNPDYDEN
                     LAMNEFLEGYYGAAAPLIRKYIDLLHDRVEKENIHCSIWIPPTHNHLTDELLVEADQL
                     WQAAEEAVKDDPTLLKRVRIGRMSVDYAILERARTAPDKEPLGSLAKVRFQPFIDMIQ
                     PAGVTRLNEWNPLNLDEYRTKLASALRIKP"
BASE COUNT          313 a          365 c          381 g          333 t
ORIGIN      
        1 cctaacccat ctggccaata tcgctgtgcg tgtggggcgg gctctgcggg tcgatcctca
       61 acgggaaaca attatcggtg atgaagaagc cagccggctt ttgcgacgaa actaccgaga
      121 aggtggccat tgggcgattc ccaagggcct gtgatcgagt tgtccaaagc ccacagttag
      181 accgaatgac atcacgcctt ttggggagat gctgagccgc ttcaattacc gtccaggccc
      241 aaccgccccg acggcaattg ttcgcaggat gacaatcggg cagcgatatc ctggctttag
      301 ggtttgatcc gcaaggcact ggccagcttt gttcgatatt catcgagatt gaggggattc
      361 cactcgttga gccgcgtcac gcctgccggt tgaatcatgt caataaacgg ctggaaacgt
      421 accttggcca gggagccgag cggttcctta tcgggggccg tgcgggcacg ctccaggata
      481 gcataatcca cactcattcg accgatgcgg acccgtttga ggagcgtagg gtcatccttg
      541 acggcctctt cagcagcctg ccatagttgg tcggcctcca ccagcaactc gtcggtcaaa
      601 tggttatggg tgggtggaat ccagatgcta cagtgaatgt tctctttctc aacacgatcg
      661 tggagcagat cgatatactt ccgaatcagg ggggcggccg ctccgtaata tccctccagg
      721 aattcgttca tggcaaggtt ctcgtcgtaa tcaggattcc aaaggaactt ggccgtcatg
      781 taaccaccga gggccgccaa ttcgctgttc ggcgtgttgt aggtatcctg ctcaaagata
      841 cctttcacgt tgtttgccac aaatagtttg atgttatcgt tacggacccg ctggttggga
      901 aacggcagat aatagtgccg aaaatcggtg acataatccc agacccagag ccggttcccg
      961 atcttggccc agccttgaag gtcttccaca aatgcccgat tggccggtaa atcgcaagtc
     1021 gccaaaggat gcgaaaagca acattcgatg gaacacagcc ggataatcac attcggtcgg
     1081 ggacgcattg ttttcggtgg tttgcgtgtc cactgatagg ccaaggtctc gatggccttg
     1141 tctggataac gtttttcgac agcttcagcc acgcggttaa ccatttgaag gacgggggcg
     1201 atttggctct cttcggcctt ggcgagggct tgacagtttt cacattcaca atagttgtac
     1261 caatcgttct gagacaccga gaatacaaac gcctccgaat cgttttcgat ggccttgagg
     1321 atcccttccg taacaagccg gataacatcc tcattggtgc aacataactg cgtgcgagtc
     1381 ttaaggcgtt tc
//
LOCUS       DAOPTR010001088         1390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_73504_length_1390_cov_3.216479, whole genome shotgun sequence.
ACCESSION   DAOPTR010001088 DAOPTR010000000
VERSION     DAOPTR010001088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73504_length_1390_cov_3.216479"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1096..>1390)
                     /locus_tag="PKI05_15955"
     CDS             complement(1096..>1390)
                     /locus_tag="PKI05_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53746.1"
                     /translation="TAGGEQRDTNQNGADHDTACHGYISSGWEGPTETSYLPKLRDAF
                     LSPWDHRGKSETYLTHSVNTLVQFRTFSTVMLSHLMKDLEVGLLGRFVGGNFT"
BASE COUNT          399 a          376 c          357 g          258 t
ORIGIN      
        1 ggccatgtct cgtggaaggc tcagatgagt tatacggagg cggccctcgc gatcggacac
       61 ctcatgagcc gtggcgcaaa ggcctcagca tgcgatcgct tcggcgagag attcccgcaa
      121 atcatctggt aaagcggaaa tcaatcacgg tatctatagc gcgacgtctc ctcgctgctc
      181 gacaatgcca aagaactgga ggctccgtcc cgtgcatcaa tttggggttg cgttcgggaa
      241 aaactctcgg taaaagtttc ccccgttgta cgactaaaag acggcgggcg agaaaaagcc
      301 cgaaggaaca aaggaaaaac gttaaccgct tgtgccgaga tttccgaaga tgtcaaaggg
      361 gacatgcaaa tcggctgtcc cgaatacaac aaagaatctc gaaaaatggc cttgcggtga
      421 gtcgtaggaa aggctaaaac ccaaccccaa caacgcggct aacaataaca accgttccag
      481 gcgaaagccg gccgcgtgat gcacaccaag aggaaatcag ttctgagaat tatcacctcc
      541 ctgaaaatac ccacctaaac attcccccct gtggacagcg gcagacctat cttgggaaga
      601 gagtcgtgaa cagcaggttg ccgcagcgga aacacaataa gtgtcacacc ataggatgtg
      661 aagaacatcc gccttcctgc cggtcggtga aatctcccgc ctaaccgacc ccctgaggca
      721 tcgcctccgc cctgggaggc cgtcgtgggt ccggcggtct cccaggccga cgatcgaagt
      781 gaaatcgacc gacacaacac gaggacgata cccagttacg gacaagcagc gaacaataaa
      841 ccgaagcagt gagaattagg gttaacacac tactacacaa gagaacatac acaaggtgag
      901 gcggtcaaac gacctcgcca ccagcaaaag agaaaacaac actcgggtct gaaaagcgga
      961 atcgtcatcc gagccaagaa aggaggtgct acgacaagga cagagagtga ctgtggcatg
     1021 tgacgagaag ttcaccccga acgactaggg taaaggtcgg ctgcgtgctc caaagaaaag
     1081 atccatgttc ctcccctaag taaagttccc accgacaaac cttccaagaa gtcccacctc
     1141 caaatctttc atcaagtgcg acagcatcac cgtagagaaa gttcggaatt gaactagggt
     1201 attcacacta tgtgtcaagt atgtttccga ctttcctcgg tggtcccacg gtgacaaaaa
     1261 agcgtcccta agttttggta agtaagacgt ttctgttggt ccttcccagc cagaggagat
     1321 gtagccatga cacgccgtat cgtgatctgc gccgttttgg ttggtatcgc gttgttcgcc
     1381 acccgccgtg
//
LOCUS       DAOPTR010001089         1389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_73576_length_1389_cov_3.946027, whole genome shotgun sequence.
ACCESSION   DAOPTR010001089 DAOPTR010000000
VERSION     DAOPTR010001089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73576_length_1389_cov_3.946027"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PKI05_15960"
     CDS             complement(<1..516)
                     /locus_tag="PKI05_15960"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOA53747.1"
                     /translation="MAWLWVAVMVLATETNANTITLANCPVSIIDEAQVPARVQGQLI
                     ELSVKEGDSVTKGDVLGRIDDAVLRKTLEVKQHQLAAAQKEADNDVNVRYAKWANLVA
                     DAEYQQAVDANNRAPGAVPQAEMRRLLLERGKTSLAIEQAAHDLEVAKLTVQVRQAEL
                     AEVEEQLEMYRI"
     gene            complement(563..>1389)
                     /locus_tag="PKI05_15965"
     CDS             complement(563..>1389)
                     /locus_tag="PKI05_15965"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOA53748.1"
                     /translation="ASCPGTLEPVLRRDVFAGIDGIVDEVLVVHGQHVNQGDILARLR
                     NSELKQQILEIEGQLAANQKQVATIRRELADLRMLSAQERNRLTGQLAELEARQISLA
                     AQLAILREKEKELTVLSPISGDVVTWDVYNRLINRPVNRGQALMRIADTSGEWELELH
                     MSEDRMGEIALARQQVKAQDPSQDLTVEYVLATDPGRTRTARVKDVYVSAEVLPEEGN
                     VVMIKVAVNKDDFDATQLRPGATVSAKVHCGTRSMGYAWFRDLIAFIQTRVLFYLW"
BASE COUNT          261 a          429 c          364 g          335 t
ORIGIN      
        1 gatgcggtac atctccagtt gttcttcgac ctcggccaac tctgcctgtc ttacctgaac
       61 tgtgagcttg gccacttcca gatcgtgggc ggcttgctca atggccaggg aggtctttcc
      121 ccgttccagg agcaagcggc gcatttctgc ctgggggaca gctcccgggg cccgattgtt
      181 ggcgtccact gcctgttgat actccgcatc ggccacgaga ttggcccatt ttgcataacg
      241 gacattgacg tcgttatcgg cctctttttg ggcggctgcc aattggtgtt gtttgacctc
      301 cagggtcttg cggaggactg cgtcatctat gcgtccgagc acatcgccct ttgtgacgga
      361 gtcgccctct ttaacgctca gttcaatcag ttgtccctgc acccgggcgg ggacctgggc
      421 ttcgtcgatg atggagaccg gacaattggc cagcgtgatg gtatttgcat tcgtttccgt
      481 cgccaaaacc atcaccgcaa cccacaacca agccatatgc cactccgaaa tctttggtct
      541 cgtatttcct caactcaacg gatcaccaca gatagaacaa gacacgcgtt tggataaatg
      601 cgatcaggtc gcggaaccaa gcgtatccca tcgaccgcgt tccacaatgg actttggcac
      661 tgacggtggc cccgggccga agttgtgtgg cgtcgaaatc atccttgttg acggccacct
      721 tgatcatgac cacgtttccc tcttcgggaa ggacctcggc cgacacataa acatctttca
      781 cccgcgcggt ccgcgtccga ccagggtcgg tggccaggac atattccacc gtcaagtcct
      841 ggctcggatc ttgtgctttc acctgttgcc gggccaaggc gatctcaccc attcggtctt
      901 ccgacatgtg gagctccagt tcccactcgc ccgacgtgtc ggcaatccgc atgagggctt
      961 gcccacgatt gaccggacga ttgatgagcc gattgtacac gtcccacgtg acaacatcgc
     1021 cgctgatggg actgagaacg gtcaattcct tttccttttc ccggaggatc gccaattgtg
     1081 ccgcaagact gatctggcgt gcctccaact cggcaagctg gcccgtcaga cggttccgct
     1141 cctgggcact taacatccgc agatcggcca gttctcgccg gatggtggcc acctgctttt
     1201 gattggcggc gagctgtcct tcgatttcga gaatctgttg tttcagttca ctattccgca
     1261 aacgggctaa gatgtcgccc tgattgacgt gttgcccgtg cactaccaac acttcatcca
     1321 caatgccgtc aattcccgcg aacacatcgc gccgcaggac aggttccagt gtccccgggc
     1381 atgaagctt
//
LOCUS       DAOPTR010001090         1388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_73641_length_1388_cov_4.134284, whole genome shotgun sequence.
ACCESSION   DAOPTR010001090 DAOPTR010000000
VERSION     DAOPTR010001090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73641_length_1388_cov_4.134284"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..927)
                     /locus_tag="PKI05_15970"
     CDS             complement(70..927)
                     /locus_tag="PKI05_15970"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOA53749.1"
                     /translation="MHAKSRFGIGVTWLGKVAILSAAFSFLLGLTPAVAEESSALPPV
                     ESLLPPDPLPALPDGKKWQLVWHDEFEGLQIDTTKWEIYGDWKRRDGYWVKEDSFLDG
                     KGHLVIRTKKSGDRYTSGAIATKGKFEPRFGYLVCRCTHPKQPGMWAAFWMFGSGVTR
                     VGDEGRDGTEIDIMEMPYRDGRLTSNLHWDGYGKDHKHAGTTFRMPEILEGFHTYGLW
                     WKPDEYVFDVDGKETWRTSAGGVCQVPLYIKLTVEIGSWAGDIKTAQLPEDWLIDYVR
                     VYQMFDKEP"
BASE COUNT          322 a          400 c          338 g          328 t
ORIGIN      
        1 ctaactacga gatccccgct tcgcaatgca cttttgcgca aatcgatatc acttttgcgc
       61 aaaaaccgat cacggctcct tatcgaacat ttgatagacg cgcacatagt caatcaacca
      121 atcttcggga agttgggccg tcttaatgtc tccagcccaa gagccgattt cgacggtcag
      181 cttgatgtag agggggacct gacagacccc tccagcgctc gttcgccacg tttctttccc
      241 atcgacatcg aagacatatt cgtccggctt ccaccaaagc ccataggtgt gaaagccctc
      301 cagaatctcg ggcatccgga aggtcgtccc cgcgtgtttg tgatctttac cgtagccgtc
      361 ccaatggaga ttgcttgtca aacgcccatc gcgatagggc atttccatga tgtcgatttc
      421 ggttccatca cggccttcat cgccgacacg tgtcacgccg gacccaaaca tccaaaaagc
      481 cgcccacata cccggttgct tgggatgcgt gcaacggcaa accaggtacc caaaacgggg
      541 ttcgaacttg cctttggtgg caattgcccc ggaggtgtag cggtcgccgg actttttcgt
      601 gcggatgacc aagtgtccct ttccatccaa aaatgaatct tccttgaccc aataaccatc
      661 tcgccgtttc caatcgccat aaatctccca ttttgtcgta tcaatttgaa gtccctcaaa
      721 ctcgtcgtgc caaactaatt gccacttttt gccatcgggc aaagcgggga gtggatcggg
      781 cgggagcaag gattccaccg ggggcagggc gctcgattcc tcggcgacgg cgggcgtcaa
      841 tcccaacagg aaactgaaag ccgcagataa gatcgctacc tttccgagcc aagtgacacc
      901 gatgccgaaa cgggattttg cgtgcataat atggctctcc tttttcgctg ctcgatccaa
      961 cacaatatgc tggtcccacc ggcttgtgga acggccttgc ggaaaaacga cacggttact
     1021 tgcgctttgg cgagatacta ggccctacct gcactgtgaa gcatatccgg gaaaaagata
     1081 gcaaaccgaa ctatttttcc cacgattgtt cttctctcgc aggcgttgga taaaaatgac
     1141 tttctgcgtg cgacaaacag cacgctgaca attgtcacaa aggaccgtgc cgctttcaag
     1201 ccatgtgttt cccttgaccg gttggtccta aagacgacct aacttgccga cttgctggcg
     1261 gtctcctgct tggccttttt caggtatcgg tcaaggatga tgggaatctg gtccggcgtg
     1321 accgttccat gcatgtcatc attgataagc atcacaggtg ccaggctgca agcaccgaga
     1381 cagcgggc
//
LOCUS       DAOPTR010001091         1385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_73915_length_1385_cov_2.375188, whole genome shotgun sequence.
ACCESSION   DAOPTR010001091 DAOPTR010000000
VERSION     DAOPTR010001091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73915_length_1385_cov_2.375188"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PKI05_15975"
     CDS             complement(<1..841)
                     /locus_tag="PKI05_15975"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA53750.1"
                     /translation="MRRTNFSLSRRDCLKCGATAAIAGLVLPSRLVFSYEANEKVNIA
                     VIAVGGRGGANLKGVSSENIVALCDVDRRALESAAKRFPAARTYTDYRKLLETEKTLD
                     AVVVSTPDHMHAPICLQAMKQGLHCYCEKPLTRTVEEAYQMAKVASEKKLVTHMGTPG
                     RGSADTVRIVEILRSGAIGDVTEVHYWTDRPIWPQGFDRPQGEDQVPAYLDWENWIGC
                     APMRPYKEKWPEGHPVYQLPARQLHSGWVYHPFVWRGWWDFGTGALGDIAPHMWHSAW
                     WGLE"
     gene            1100..>1385
                     /locus_tag="PKI05_15980"
     CDS             1100..>1385
                     /locus_tag="PKI05_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53751.1"
                     /translation="MSAEQQPPIAKQACCVLEVPEKSLRLPVRAALPAGCSFSESAHC
                     TVVSQAPDLKVSAEVLGGPDANGELRAPGYLVLRSTSAEKVSQDKVQPISL"
BASE COUNT          284 a          417 c          368 g          316 t
ORIGIN      
        1 gctcaaggcc ccaccaagcc gagtgccaca tgtggggtgc gatgtctccc aaggctcccg
       61 ttccaaaatc ccaccaacca cgccaaacga agggatgata cacccaacct gagtgcaact
      121 gcctcgctgg tagctgatag actggatgcc cttccggcca cttttccttg tagggcctca
      181 tgggtgcgca tccaatccag ttctcccaat ccagataggc cggaacctga tcctcaccct
      241 gaggacgatc aaacccctgc ggccaaatgg ggcgatccgt ccaataatgg acctccgtca
      301 cgtcgccgat tgcgccactc cgcagaatct cgacaatgcg gaccgtgtcc gcacttccgc
      361 ggcccggcgt ccccatgtgc gtaacaagct tcttctcgct cgccaccttg gccatttggt
      421 aggcctcttc caccgtcctg gtaagaggtt tttcgcaata gcagtgcaaa ccttgcttca
      481 tcgcctgaag gcagatggga gcatgcatgt ggtcgggcgt gctaaccacc acggcatcca
      541 atgttttttc ggtctccagc aacttgcggt agtccgtata agtccgcgca gccggaaatc
      601 gctttgccgc ggattcaagt gcccgacgat ccacatcaca aagtgcgacg atgttctcgc
      661 tgctcacgcc tttcaaatta gctccacctc gtcccccgac cgcgatgacg gcaatattga
      721 ccttctcatt ggcctcgtaa gagaaaacga gtcgcgatgg caaaacaagg cccgcaatcg
      781 cagccgtcgc tccgcacttg agacaatccc gccgagacag actgaagttg gttcttcgca
      841 tggatggttc tccttatctg aacgggcact ttgtgaggaa ggcatcacta tgacacgcag
      901 tggcaaatgg gaaggtaacg tcaccggggc tacgggtgtt ccgttgcaat cgcccgtgat
      961 tataatcact ggttgtttgt ggtgacaagc gactgggtat tgaggcttgg tagggttcga
     1021 tatgcgaaac gattgctact ggttgggtgt tcgatttctc gtcgccttac tcacgtgggc
     1081 ttgtttacga tcggtcgttg tgtctgccga acagcaaccg cccattgcca agcaagcctg
     1141 ctgtgtgctt gaggttccag agaagtccct acgcttgccg gttcgcgcgg cactgcctgc
     1201 cggctgttca ttctccgaga gcgcgcattg cactgtcgtc tctcaggcac cagacctgaa
     1261 ggtctccgcg gaggtcctgg gtggcccgga tgctaatggg gaactccgtg caccagggta
     1321 tctagttctc agaagtacat ccgccgagaa ggtgtctcaa gacaaggtcc agcccatttc
     1381 tctgc
//
LOCUS       DAOPTR010001092         1384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_73966_length_1384_cov_3.851768, whole genome shotgun sequence.
ACCESSION   DAOPTR010001092 DAOPTR010000000
VERSION     DAOPTR010001092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73966_length_1384_cov_3.851768"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="PKI05_15985"
     CDS             complement(<1..1078)
                     /locus_tag="PKI05_15985"
                     /inference="COORDINATES: protein motif:HMM:NF026066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOA53752.1"
                     /translation="MTNAEIAAVFEQIADLLEFQGANPFRVRAYRTAARNIGELTEPL
                     AEIVAAGDRDLTELPGIGQDLAEKIVTLLKTGSLPLLEVLKSQVPESVLTLLRVPGLG
                     PKRAAALYKKLQITTLEELRQACLDHRVRELEGFGSKTEENILKGIEAAIQAGQRILW
                     YEADQIVQSLREHLEPTAGLHRLVPAGSYRRGKDTVGDLDFLALADTPDPVMDRLATF
                     RGVKDILARGDTKMSIVLQNGIQVDLRVVPAESFGAAQQYFTGSKEHNVILRGMAKNR
                     GLKINEYGVFRGEEQIAGREEEEVYAALDLPWFPPELREGRKEFEWAAAGKLPDLITL
                     DDIRGDLHIHSTWSDGMATIEEIAE"
BASE COUNT          276 a          416 c          367 g          325 t
ORIGIN      
        1 cttcggcgat ttcttcaatg gtggccatac catccgacca agtactgtgt atgtggaggt
       61 cccctcggat gtcgtccagg gtgattagat cagggagttt gcctgccgca gcccattcga
      121 attctttgcg accttcccgc aattccggcg ggaaccaggg caggtctaaa gcggcataga
      181 cctcctcttc ttcccgtccg gcaatctgtt cttccccgcg aaacacccca tattcgttga
      241 tcttaagccc acgatttttc gccattccgc gaaggatgac attatgctcc ttcgaaccgg
      301 taaagtattg ctgggcggcc ccgaacgact cggccggcac tacccgcagg tccacctgaa
      361 taccgttttg caggacgatc gacatcttag tatcgccgcg ggcaagaatg tccttcaccc
      421 cacgaaatgt ggccaagcga tccatcaccg ggtcgggcgt atcggccaga gcaaggaaat
      481 ccaggtcgcc cacggtgtcc ttacctcgcc gatagcttcc tgctggcacc agccgatgca
      541 ggccggctgt tggctcaagg tgctcccgca gcgattggac aatttggtcg gcttcgtacc
      601 aaaggattcg ctgtcccgcc tgaatggccg cctcgatccc cttaaggatg ttctcctcgg
      661 tcttacttcc gaatccttct aactcccgaa cacggtgatc gagacaggct tgccgtagct
      721 cttccagtgt cgtgatttgg agctttttat agagggcagc cgcacgcttg ggacccaaac
      781 cagggacgcg aagcagcgtc aagacgcttt cgggcacctg ggactttagc acctcaagca
      841 gaggaagaga gccggtcttt aagagcgtga caattttttc tgccaagtct tgcccaatgc
      901 cgggcaattc cgtgaggtcc ctatcgccgg cggcgacaat ctcggccaat ggttctgtca
      961 gttccccgat attccgcgcg gccgtgcgat aggcccttac ccgaaaggga ttggccccct
     1021 gaaactccag caggtccgca atttgctcaa agacagccgc aatttcagcg ttggtcacgg
     1081 tgattgaaac tccgaatgat tacgtatccg tttattccgg acgtgctttg gtggccaatt
     1141 attcctctgc atgtgccagt tctatgagca cgaggcccgc tttggtccgc cgggccacgg
     1201 taacatgcgt tacatatgca attccacgat gtcccgatct tgcaacacgt agtctccctt
     1261 gaccactgtg cccggatgca cggcggttcc ccagacgcgg gcgaatttca agtttttggc
     1321 caagtccttg tgaatttccg aggccatgtc ggcgacagtg ctcccccggc ggaaggtgaa
     1381 gggg
//
LOCUS       DAOPTR010001093         1381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_74222_length_1381_cov_2.647813, whole genome shotgun sequence.
ACCESSION   DAOPTR010001093 DAOPTR010000000
VERSION     DAOPTR010001093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74222_length_1381_cov_2.647813"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            383..>1381
                     /locus_tag="PKI05_15990"
     CDS             383..>1381
                     /locus_tag="PKI05_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53753.1"
                     /translation="MKQIRPKGFLGVFFFLIWCLGIWCSAARAADLKGEAYVGRPFGV
                     GKIELVITPDILPAPLGVEGILLKEANGRVHYPVVATSAVGSFFAGLVGAPPRATFYF
                     LFRGDEPLEIELMARAPIRSILRPRSDPRQWNRLLSAWWREYSQPPGLFESKPDYPPL
                     VAQYLRLMLAQRLGLRPPRLSSQSWEVWFREQLGLTLSSESMRVSMMETRFFDPAAVT
                     SPADQDLPDPWDNQPSSLPDIPANVAIEPIASHVPVECLYIRFGSFRNFLWLQDTLAR
                     WGGDIQNLVALRSLNYQMGQKIEQQLVLRQSALSRLFGETVIADVAIIGYDLYFHEG"
BASE COUNT          256 a          391 c          391 g          343 t
ORIGIN      
        1 cgcctagcct ggtttgctcc gtgggtcttt ctcatgggtt tcctcgtcct catttggcaa
       61 gtgtcaatct ttttctaaga gcctatccca caacctcccc tcgcctgttc ggcgggaaag
      121 gtgccaaggg agagggtggc tcgaatcgct gctggttctc tcgatcgact ccgacgattg
      181 cttgccctca ccccaaccct ctcccaaagg gagagggaac ttttggatag gctttaagtt
      241 agacatggat cactctcctt gctggtggct tatccgctgt tgtctctcag tccctcgtgc
      301 ggcagaatga gggattgcgg gaatcgcacc gggacacggt caattccgcc taccaaactg
      361 atttcagcca cggaggaaca aagtgaaaca aataaggccc aagggatttc tcggcgtctt
      421 cttctttctc atttggtgtt tgggaatctg gtgttcggcg gcaagggctg ctgatctcaa
      481 aggggaggcc tacgttggaa ggccgtttgg cgtgggtaag atcgaacttg taataacccc
      541 cgatatattg ccggctcctc tgggtgttga gggtattttg ctcaaagaag ccaacggtcg
      601 ggtccattac cctgtggtgg cgacgtcggc cgtgggtagc tttttcgccg gattggtggg
      661 cgctccgcca cgcgccacat tctatttcct ttttcgggga gatgaaccgt tggaaatcga
      721 gctaatggcc cgggcaccga ttcgctcgat cctgcggcct cggtcggacc cgcgacagtg
      781 gaaccggctt ttgtccgctt ggtggcgcga gtacagccag ccgccagggc ttttcgagtc
      841 caagcctgac tatcccccgc tggtggcaca gtaccttcgg ctaatgctgg ctcaacggct
      901 tgggcttcgc ccaccacgtc taagttccca gagctgggag gtgtggttcc gagagcaatt
      961 gggcctgacg ctgagcagcg aatcgatgcg agtgagcatg atggagaccc gcttcttcga
     1021 cccggcagcg gtgacaagtc ctgcggatca agacctgcct gatccctggg ataatcagcc
     1081 ctcatcgctc cccgatatcc cggccaacgt ggcaatcgag cctatcgcct cccacgtccc
     1141 cgtcgagtgt ctttatatac ggttcggcag ttttcgcaat ttcctgtggc tccaggacac
     1201 actcgctcgt tggggtggtg atatccaaaa cctggtggcc ctgcgctcat tgaattatca
     1261 aatgggccag aagatcgaac agcaacttgt actgcgtcag tcggccttgt cccgcctttt
     1321 cggcgaaacg gtcatcgccg acgttgccat cattgggtat gacctttact ttcacgaggg
     1381 g
//
LOCUS       DAOPTR010001094         1373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_74888_length_1373_cov_1.974203, whole genome shotgun sequence.
ACCESSION   DAOPTR010001094 DAOPTR010000000
VERSION     DAOPTR010001094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74888_length_1373_cov_1.974203"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="PKI05_15995"
     CDS             complement(<1..161)
                     /locus_tag="PKI05_15995"
                     /inference="COORDINATES: protein motif:HMM:NF013878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOA53754.1"
                     /translation="MAKKKAAIPKSSGTEKQKKLVIVESPTKARTISRFLGDEFVIEA
                     SLGHIRDLP"
     gene            778..>1373
                     /locus_tag="PKI05_16000"
     CDS             778..>1373
                     /locus_tag="PKI05_16000"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOA53755.1"
                     /translation="MASPFKVFRKNAKVLLVGLFLLSMISFVVIPSFLQWLETRRGHT
                     VRSVVSTKKFGDLSEMDLHNLWIEHNTVRRFLEALGRRIDESRGSSFRIRYFLAVMGE
                     ATEEQLVQTWLFSQYAEIAGVKVTDEVINEFIRGLINASGVSISPADLAQLLSQNQIQ
                     EDTLFRGLRREILVLRYLQLAGFDMFSLQPGFAGETPA"
BASE COUNT          294 a          357 c          362 g          360 t
ORIGIN      
        1 tcgggcaggt cgcgaatatg gcccaggctc gcctcgatga cgaactcgtc gcccaagaag
       61 cggctgatcg tgcgagcctt ggttggcgac tctacaatga cgagtttctt ctgtttttcg
      121 gtcccagaag atttggggat agctgctttc ttttttgcca tttctcacca aaaatcggat
      181 attatttgct cgataacacc ctcgcctaca aatttactgg cctaagggct tgcccgttac
      241 catttgcccc taatacattc tttgccgtgc caaggagctg ggcgcacccg aagtaccacc
      301 cgatttgtga taacctgtat tggaaatctt tccaagacca cctggaaacg gaaaggtgca
      361 ctctggacct atataaccca ccctagcctg ggggattccc gtgggcggac gctcgggacg
      421 tcgtttaaat ccgccaaata gcgcgaggga aaactgcttt aacccgtctc aaacaccgca
      481 cgacagggcc gtagcaaact ggcacccaaa ttctttctga agcgcatcgt gggctttttg
      541 acaaggcttg ccgaaagcgc agatggcatt tccatttcgt cgccgagaac ttagaatgat
      601 ggttcttggc agtttctgca attcctcgcc tccaggggcg aggatggata tcgctacggc
      661 ccggccgacg ggccattttg tggttgctaa acgccccctt gcgggtggag ttcgggttaa
      721 gttggagcct cgttcttgac gaggggttga atttagcatt acggaagcga tcccgttatg
      781 gcaagcccgt ttaaagtttt ccgcaaaaac gcaaaggtcc tgctggtcgg actctttctc
      841 ctttcgatga tcagcttcgt cgtcattcca agttttttgc agtggttgga aacccgccga
      901 ggccacaccg tccggtcggt cgtttccacc aagaaattcg gcgatctatc ggaaatggat
      961 cttcacaacc tatggatcga acataatacg gtccggcggt ttttagaggc gctcggtcgg
     1021 agaattgacg aatctcgcgg aagttctttt cgcattcgct attttctcgc cgtgatgggg
     1081 gaggcaaccg aggagcaact cgtgcaaacc tggctgtttt cgcagtatgc cgaaattgcc
     1141 ggagttaagg ttacagatga ggtgatcaac gaatttattc gggggctgat caacgcctca
     1201 ggtgtgtcga tttccccggc tgatttggcc cagttactgt ctcaaaacca aatccaagag
     1261 gatacgctgt ttcgcgggct tcgacgggag atcttggtcc ttcggtatct ccagcttgcc
     1321 gggtttgaca tgtttagttt gcaacccggc tttgctggtg aaacccccgc tat
//
LOCUS       DAOPTR010001095         1370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_75105_length_1370_cov_2.525475, whole genome shotgun sequence.
ACCESSION   DAOPTR010001095 DAOPTR010000000
VERSION     DAOPTR010001095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75105_length_1370_cov_2.525475"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /locus_tag="PKI05_16005"
     CDS             complement(<1..1320)
                     /locus_tag="PKI05_16005"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOA53756.1"
                     /translation="MVRKLTCPLLAIVTWLLLCFAAPTWMPSCPRFTISPGVAMAQGR
                     GEDLATALEESINRAIQYLKKQQRDDGSWPEPINMPCGMTGLCTLALLSAGVSADDPT
                     IAKALRYLRRFSPSDIDKTYSITLQTMVFCRAEPARDAPRILENIRWLERTQIKDDPD
                     RGGWSYPGSSADNSNSQFALLALYEAERVGVRASDQTWTFAKEYWEGTQNPDGSWGYR
                     RGQAGTGSMTCAGIASLVIIRDIAYQPTATVDANNRINCCGSSPQSVDRVQRGLAWLE
                     RHFTVRQNPQAGSSWLLYYLYGLERVGRLSAQRFIGPYDWYREGSAFLLELKSTVAQG
                     GIPTDYWVGPGLENDPLLATSFALLFLAKGRRPVLVTKLKYGNAENWNRHRNDVNNLA
                     AYTESRWQMDLGWQVTDLMAASVEDLLQSPVLYLGGAESPLPNEAEQQ"
BASE COUNT          314 a          386 c          374 g          296 t
ORIGIN      
        1 ctgctgctcg gcctcgttgg gcagcgggct ttctgcacct cccagataaa ggactggcga
       61 ctggaggagg tcctccacgc ttgccgccat taggtccgtg acctgccacc ctaggtccat
      121 ctgccagcgg ctttcagtgt atgctgcgag attgtttaca tcgtttcggt ggcggttcca
      181 attttctgca ttcccatatt tcagtttggt gacgaggaca ggacgtcgtc ccttggcgag
      241 gaagagcaag gcgaaagacg tggcaaggag gggatcgttt tccagtcccg ggccaaccca
      301 atagtcagtc ggaataccac cctgggccac ggttgacttc aattccagga gaaaggcaga
      361 gccttcacga taccaatcat aaggcccaat gaatcgttgc gcactcaggc gaccaacacg
      421 ctccagacca taaagatagt aaagcagcca ggaactccct gcttgcggat tttgcctaac
      481 cgtgaaatga cgctccagcc atgccagccc tctttggacg cgatccacac tttggggaga
      541 agaaccacaa caattgatcc gattgttggc atcgacggtg gccgtaggtt gataggcaat
      601 atcccggata atcaccagtg aggcgatccc ggcgcatgtc atactgccag tgccggcctg
      661 accgcggcga tagccccacg atccatcagg gttttgggtg ccctcccaat actccttggc
      721 gaaggtccac gtctgatcgg aagcccgtac gccaacccgt tccgcttcgt acagggctaa
      781 aagggcaaac tggctattcg aattatcggc gctgctgccg ggatacgacc aaccaccacg
      841 gtccgggtcg tccttgatct gcgtccgttc cagccagcga atgttctcca gaatccgggg
      901 ggcgtcacga gcaggttctg cccggcaaaa gaccattgtt tgcagcgtta tagaatacgt
      961 tttgtcgata tcagagggcg agaatcttcg caaataccgc agggccttag cgatcgttgg
     1021 atcatcggcg gaaacacctg cgctgagaag ggccaacgtg cacagcccgg tcatgccgca
     1081 aggcatgtta atcggttctg gccaagagcc atcatcgcgc tgctgttttt tgaggtattg
     1141 aatcgcccgg ttgatggatt cttccaaggc cgttgcaaga tcttcgcctc gcccctgcgc
     1201 catggctacc cccgggctaa ttgtgaaacg cgggcatgat ggcatccagg tcggtgccgc
     1261 aaaacaaagg agtaaccagg tcacgatcgc aagtaatggg caagtcagtt ttctgaccat
     1321 tcggtcacgt tacgaaaaat gtcaacggcg gtttcaataa aaacgacaaa
//
LOCUS       DAOPTR010001096         1369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_75193_length_1369_cov_2.818113, whole genome shotgun sequence.
ACCESSION   DAOPTR010001096 DAOPTR010000000
VERSION     DAOPTR010001096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75193_length_1369_cov_2.818113"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /locus_tag="PKI05_16010"
     CDS             complement(<1..1321)
                     /locus_tag="PKI05_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53757.1"
                     /translation="MNWRRRKHSKSGENPISLFPFLAVLICTMGVLILLLVILVRQAR
                     LKALAQAQASSNPASQKEVSSPIPTEDELKRWEEDLAWQEEQLRTAREKTQAQLTEAR
                     LILGHIEDSIRTFRQELQSLQERLQRLNEVTNESGSVSIAELENHLHELHEAAQSLKA
                     QVEAKEHQRRRKSYAIIPYQGKFGTARRPIYLECRGDGILLQPEGIRFTVQDFLGPLG
                     PGNPLDAALRAIREELLARKEFDPQADGEPYPLLIVRPDGIEAYYAARAAMSSWGTEF
                     GYELVEKDWELVYPQPDPRLAEVVQQAVDLARKRQEFLVKAAPGKYGRTPRQYRAAPY
                     VGGAVPTEAPATPPEDPMLAQWLARQQSGNTAVRPESAPGGAAATSRTGNSTSEQAST
                     QSPVERQVEDHVTISDLRVMSPHRPVGDQPQVGGAPSPKESTAVEITT"
BASE COUNT          221 a          377 c          457 g          314 t
ORIGIN      
        1 ccgtcgtgat ctccacagcg gttgattcct tgggtgaggg ggcaccgccg acctgcggct
       61 gatcaccgac aggccggtgt ggcgacatca cacgaaggtc ggagatggtg acgtggtcct
      121 cgacttggcg ctcgacaggc gactgggtgg aggcttgctc cgacgtcgag ttgcccgtcc
      181 gtgaggttgc ggccgcgccg ccgggcgcgg attctggacg caccgcggtg ttaccggact
      241 gttgccgggc tagccattgg gcaagcatgg ggtcttcggg cggtgtggct ggtgcttcgg
      301 tggggacagc cccgccaaca tacggcgctg cgcgatactg ccgaggtgtt cgcccatatt
      361 ttccgggcgc cgccttgacg aggaattctt gtcttttgcg ggcaagatcg acagcttgtt
      421 gaacgacctc agccagtcgg ggatcgggtt ggggatagac caactcccag tccttctcca
      481 ccagttcgta gccgaattcc gttccccaag aagacatggc ggcacgtgcc gcgtagtagg
      541 cctcgatgcc gtccggccgc acgatcaaaa gaggataagg ctcgccatcg gcttgggggt
      601 cgaattcctt tcgggcgagg agttcttctc ggatcgcccg cagggccgcg tccagcgggt
      661 tgccgggccc tagtggcccg agaaaatctt gcaccgtaaa gcgaattcct tccggttgca
      721 ggaggatgcc gtcgccgcgg cattccaggt aaatggggcg acgggcggtg ccgaatttgc
      781 cctgataggg aatgatggcg taacttttgc gacgccgctg gtgctctttg gcttcgactt
      841 gtgccttgag actttgtgcg gcttcgtgca gttcgtggag gtggttttca agttcggcaa
      901 ttgatacgct tccgctctcg ttagtgactt cattcagccg ctggagccgc tcctgcaagc
      961 tctgaagctc ctggcgaaag gtacggatgg aatcctcaat gtgtcccaag atcaggcggg
     1021 cttcggtaag ttgggcctgc gtcttttccc gggctgttcg cagttgttct tcctgccagg
     1081 ccaaatcttc ttcccagcgc ttaagctcgt cttccgtggg aattggggag cttacctctt
     1141 tctgggacgc aggatttgat gaggcttgtg cctgggcgag ggcctttagg cgggcttggc
     1201 gaacgaggat gaccaatagc aggataagga cccccatcgt gcagatgagc acggccagga
     1261 aggggaacag cgaaatgggg ttttcgcccg atttgctatg tttgcgtcgt cgccagttca
     1321 tgccgcttct gcccgtcggg gtcgttccgc ccggggtggt tccaagggg
//
LOCUS       DAOPTR010001097         1368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_75260_length_1368_cov_3.565880, whole genome shotgun sequence.
ACCESSION   DAOPTR010001097 DAOPTR010000000
VERSION     DAOPTR010001097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75260_length_1368_cov_3.565880"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PKI05_16015"
     CDS             complement(<1..758)
                     /locus_tag="PKI05_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53758.1"
                     /translation="MTRSVLRLCLVLGLAMISLAIASVTCAADWYVAPDGRPNNQGTL
                     DFPWDFGSALDGTHKIAPGDTVWIKAGTYRHPDRSLNSPGFVVRLQGTEEKPIHVRAM
                     PGHRVTVDGGLSVVAPSDYLWIWDVEILVSENFTMSREINEPGSHPQSYGRPWGGLNI
                     HAGKGCKYINLVIHDNAQGVSFWRGATDSELYGCIIYDNGWKAPDRGHGHAIYTQNEL
                     GVKTIADCIMTGGYGYTMHAYGSENAYVDHYLVVG"
     gene            complement(938..>1368)
                     /locus_tag="PKI05_16020"
     CDS             complement(938..>1368)
                     /locus_tag="PKI05_16020"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOA53759.1"
                     /translation="SAVIWHYRGQDLNNDGKIDFEEEFHRTIGMPAVANGLLVVGDHS
                     GVVHCLDAATGKAFWTYDMMASIWGSPLIADGKVYIGDEDGDVAVFALSRERQLLAEN
                     TVGSSVYTVPAATDGVLYIVSQRHLMAISQKKPVPSSEGP"
BASE COUNT          308 a          396 c          342 g          322 t
ORIGIN      
        1 cccactacaa gataatgatc gacatacgca ttttctgaac cgtaggcgtg catggtgtag
       61 ccgtagccgc ctgtcatgat gcagtcggca attgttttta ctcccagctc attctgagtg
      121 taaatagcgt gtccgtggcc acggtcgggg gccttccacc cgttgtcgta gatgatgcat
      181 ccatagagtt cgctatccgt tgccccgcgc cagaaactaa cgccttgggc gttgtcgtga
      241 ataaccagat tgatgtattt acaccccttc cccgcatgaa tgtttaaccc accccaaggt
      301 cttccgtagc tttggggatg cgatcccggt tcgttgattt ctcgggacat tgtgaagttt
      361 tctgagacaa gaatttctac atcccaaatc cacaggtaat cactgggagc caccacactc
      421 aagcccccgt caaccgtgac gcgatggccc ggcattgcgc gcacgtgaat gggcttctct
      481 tcggtaccct gaagtcgaac cacaaacccc ggactattca gcgagcgatc ggggtggcgg
      541 taggtaccag ccttgatcca gacggtatct ccaggggcaa tcttgtgtgt gccgtccaaa
      601 gctgagccaa agtcccatgg gaaatccagt gtcccttgat tgtttggcct cccatctgga
      661 gcgacatacc aatccgcggc acaagtgacg ctggcaatag ccaacgagat cattgccagc
      721 ccaagcacga gacacagacg aagcaccgac ctggtcatga cgtaccaact gcgtgggcag
      781 tgcgaaaatc cccattttac catatttttt gttccgttcg tacgtttccc tccgaccctc
      841 cggatgtaaa gcaattgcca aatcggtcat cgtcatgcag cggtggccat cgttccccta
      901 actgatcctt aagattaaag atgactgatc cgtaaagtta aggaccttcc gacgacggaa
      961 ctggcttttt ctggcttatc gccatcaggt ggcgctgact gacgatgtat aacaccccat
     1021 cggtggctgc cggcaccgta tagaccgaac tccctacggt gttttctgcc aacagttgac
     1081 gttctcgtga gagggcgaaa accgcgacat cgccatcctc gtcgccaata tacactttgc
     1141 cgtcggcgat gaggggcgat ccccaaatgc ttgccatcat atcgtatgtc caaaaggcct
     1201 tgcctgttgc ggcatccagg cagtgaacca cgcccgaatg gtcgccaaca accaggagtc
     1261 cattggcgac cgcgggcatt ccaatggtgc gatggaactc ctcctcgaag tcaatcttgc
     1321 cgtcgttgtt gaggtcctga ccgcggtagt gccagattac ggcagaat
//
LOCUS       DAOPTR010001098         1368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_75264_length_1368_cov_3.421935, whole genome shotgun sequence.
ACCESSION   DAOPTR010001098 DAOPTR010000000
VERSION     DAOPTR010001098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75264_length_1368_cov_3.421935"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PKI05_16025"
     CDS             complement(<1..670)
                     /locus_tag="PKI05_16025"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOA53760.1"
                     /translation="MLTSRALIEMALAGQPTPRPATGPLAVHFCAKLSGISLRQYTLD
                     PKQLARSVIEYYRRFRPDAVWLSADTWVNAEAMGARVGFPDDDQPAAGLGPALIQSPE
                     DLDAIPPPDVTRHGRWPAMLEAVRLVRQQLGDDVCLVACFDQYPFSLASQLLGLNRMM
                     LAIFDDRPFVERVMEKCLEYTLAYAVALAEAGADILSGGDSPAGLIGPRSYREVAQPL
                     EKRLI"
     gene            complement(1076..>1368)
                     /locus_tag="PKI05_16030"
     CDS             complement(1076..>1368)
                     /locus_tag="PKI05_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53761.1"
                     /translation="TFHLPEGAVLLATGSQCRHQAFRYGNNAWGLQFHVEVTPMLIRS
                     WMELATQNNELAEIGLSASQIEQETPHRFGEMQRLARIVLKRFAEQCRQCSD"
BASE COUNT          302 a          370 c          411 g          285 t
ORIGIN      
        1 caatgagcct tttttccaaa ggttgtgcta cttctcgata cgagcgggga ccgatcaatc
       61 ccgcgggcga atcaccacca cttagaatat ccgccccggc ctcggcgagg gccacggcat
      121 atgccagcgt gtattcaaga catttttcca tgacacgctc cacaaagggc cggtcgtcaa
      181 aaatggcaag catcatgcga ttgagcccca atagttgact cgccaaagag aaggggtatt
      241 gatcaaaaca tgccacaaga catacgtcgt cgcccaattg ttggcggacg agccttacgg
      301 cctccagcat ggcgggccat cggccgtggc gggtaacgtc gggcggcggg atggcgtcga
      361 ggtcctcagg cgattgaatc agggcggggc ccaacccggc ggccggttga tcatcgtcgg
      421 gaaaaccgac acgggcgccc atcgcttcag cgttgaccca cgtgtcggcg gacaaccaga
      481 ctgcatctgg gcgaaagcgg cgataatatt cgatcacact gcgggcgagt tgcttaggat
      541 cgagcgtgta ctgacgcagc gaaatcccac ttaacttggc acagaagtgc accgcaagtg
      601 gtccggttgc gggtcgcggt gtcggttgac cggccagggc catttcaatg agtgcccgcg
      661 aagtcagcat aggttgggcc ttccgttgaa attaaggctt agcgcctatc ccacaaacct
      721 cgcctcgccc gttcggcagg agaggggccg ggggtgaggg ggctcaaatc actgtcggtt
      781 ttctcgatcg actcggaaaa tcgtttgccc tcaccccaac cctctcatag gggggcgagg
      841 gaactttggg ataggctctt agcgatgaaa gatgaccaac acgtccgcca cagtttacca
      901 tatggaaatg actggatgga gacggagaga tgggaccact attgaagatg gcggatgccg
      961 gttatttgcg tattgtggcc gtggtatcga tcgtcgtgat ccagcgtgcg aaccctcggg
     1021 tggaccgcga ggcttagccg ctctctgagt caccatcagt ctgtgtgaga cacggctaat
     1081 cggaacattg gcggcattgt tcggcgaaac gtttcaggac gattctggcc agtcgctgca
     1141 tttcgccaaa gcgatggggt gtttcctgct cgatttggct ggccgagagt ccgatctccg
     1201 ccaactcgtt gttttgcgtg gccaattcca tccaagatcg gataagcata ggcgtaacct
     1261 cgacatgaaa ttgcaggccc caggcattgt taccataacg gaaggcctgg tggcgacact
     1321 gtgatcccgt tgccaggagc acagcacctt ctggaaggtg aaacgtat
//
LOCUS       DAOPTR010001099         1365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_75551_length_1365_cov_1.821374, whole genome shotgun sequence.
ACCESSION   DAOPTR010001099 DAOPTR010000000
VERSION     DAOPTR010001099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75551_length_1365_cov_1.821374"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PKI05_16035"
     CDS             complement(<1..194)
                     /locus_tag="PKI05_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53762.1"
                     /translation="MKRRGLLLGLVSWVGALVFVGAMGLFGPRADAQQGPPPHALARV
                     IAVQERHTERLMSLPGVVGTA"
     gene            441..>1365
                     /locus_tag="PKI05_16040"
     CDS             441..>1365
                     /locus_tag="PKI05_16040"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOA53763.1"
                     /translation="MLRLVFAGCLLWCWVGFSAVGAESDYFPPLESQGGWRKLDSPAD
                     IRRLAGMDPRKLEVLKEWLLVSDQRPFAAVVIRRGYIVLEVERGNSSKTDTRNIKSCA
                     KAICATVLAIASEESLAGRTPKKMTFDDPAFSFIPWAAPLSDPRKKQILVRQLLNHTS
                     GIAPEYTGAKNRGPWDYVLGHSGDPVTAKLAFDPGTDLGYSTHAFYHAALVCEDVTGV
                     PYDQFAIEKLFKPLGIERWWFAYLDGTIGRHPSHTLGLPARESARICYCMLRGGRWGD
                     RQVIPKWFVEETAAPTHSIHGIKCFNRDAESF"
BASE COUNT          307 a          386 c          399 g          273 t
ORIGIN      
        1 gccgtgccca ctacaccggg caacgacatg aggcgttccg tgtggcgttc ctgaacagcg
       61 atcacgcggg ccaaggcatg gggcggcgga ccctgctggg catcggcccg tggaccaaac
      121 aaccccatcg cgccaacaaa aacgagtgca ccaacccaac ttaccaggcc caacaacaag
      181 ccacgacgct tcataaagca cctcctaccg cgatacggca gcccagacta ttgagaccat
      241 acacccaccg agagatgggt ggttgcgtcc gacataggca cgacgtcata tcagcaccgg
      301 acggaaaatt gtcaacatgc agtggtagtg gcttgtgttt tgataaacga ccatgataat
      361 accgtcgggg gggtaaaatg gcccggcatc cctcgattca agttagctct ggccaagaaa
      421 cttttgagaa aggcgcacac atgcttcggt tagtgttcgc aggctgtttg ttatggtgtt
      481 gggtggggtt ttccgcggtg ggtgccgaaa gcgactactt cccgccgctg gaatcgcagg
      541 gtgggtggcg aaagctggat tcccccgccg atatccggcg cctggcgggc atggaccccc
      601 ggaagttgga ggtactcaaa gaatggttgc tggtgtcgga ccaacggcct ttcgccgcgg
      661 tggtgattcg ccgaggttat atcgtactgg aagtcgagcg aggcaatagt tccaagacag
      721 atacccgcaa tatcaagtcg tgtgctaagg cgatctgtgc cacggttctt gccatcgcgt
      781 cggaagagag cttggctggc cggacaccga agaagatgac ttttgacgat cccgcgtttt
      841 cgttcattcc ttgggcggca cctttgagcg atccccgtaa gaagcaaatc ttggtgcgac
      901 aactgctcaa ccacacctcg ggaatcgcac ccgaatacac gggggccaaa aaccgaggac
      961 cgtgggacta tgtcctcggc cacagtggtg accctgtgac ggcaaagctg gcgtttgacc
     1021 cagggacaga cctgggttat tccacccatg ccttttacca tgccgcactg gtgtgcgaag
     1081 acgtgacagg tgtcccctat gatcaattcg ccatcgagaa actcttcaaa cccctgggga
     1141 tcgaacgctg gtggttcgcg tatttggacg ggactattgg ccgacatccc tcccacacct
     1201 tggggctacc ggcgcgggaa agcgcccgga tctgttactg catgttgcga gggggacggt
     1261 ggggtgatcg acaggtcata cccaaatggt ttgtcgaaga gacggccgcg cccacgcact
     1321 ccatccacgg aatcaaatgc tttaaccggg acgccgaatc cttct
//
LOCUS       DAOPTR010001100         1361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_75815_length_1361_cov_3.124043, whole genome shotgun sequence.
ACCESSION   DAOPTR010001100 DAOPTR010000000
VERSION     DAOPTR010001100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75815_length_1361_cov_3.124043"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1361)
                     /locus_tag="PKI05_16045"
     CDS             complement(<1..>1361)
                     /locus_tag="PKI05_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HOA53764.1"
                     /translation="FFRGKQGPNPPDFVYIQGHAAPGIYARAYLEGRITEKHLENFRR
                     ELAEGGGLSSYPHPWLMPDFWEFPTVSMGLGPIMAIYQARFNQYLADRGILPQARESK
                     VWCFVGDGECDEPETLGAITLAGRERLDNLIFVVNCNLQRLDGPVRGNGKIIQELEGA
                     FRGAGWNVIKVIWGSDWDPLLAKDKDGLLVRRMNEVVDGQYQKYTVMPGSYIRKHFFG
                     VDPRLEALVADMTDEQIRRLKRGGHDPVKVYAAFKAAVEHKGSPTVILAKTIKGYGLG
                     EAGEGRNITHQQKKLNEEELRQFRQRFEIPIPEDQIADAPFYRPPEDSPEIQYLKARR
                     KALSGPLPARRHPMTKLKPPKWDDYAEFFAGSEGREVSTTMAFVRLLARLMRDKNIGR
                     YIVPIVPDESRTFGMEALFRQFGIYSHVGQLYEPVDSDTVLYYREAVDGQILEEGITE
                     AGS"
BASE COUNT          289 a          403 c          369 g          300 t
ORIGIN      
        1 cgacccggct tccgtaatcc cctcttccaa gatttgacca tccaccgcct cgcgatagta
       61 cagcacggta tcggaatcga caggttcata aagttgaccg acgtgcgaat agatgccgaa
      121 ctgtcgaaac agggcctcca tgccaaaggt gcggctttca tcgggcacga tcggcacgat
      181 gtatcgccca atgttcttgt cacgcatcaa gcgggcgagg agccgcacaa aggccatggt
      241 tgtagagacc tcacgtcctt cgctcccggc aaagaactcg gcgtagtcgt cccactttgg
      301 cggcttgagt ttcgtcatgg ggtgccgccg agcagggagg ggaccgctga gggccttccg
      361 gcgcgccttc aaatactgga tttcggggct gtcttccggt gggcgataaa aaggtgcatc
      421 cgcaatttgg tcttcaggaa taggaatctc gaaccgctga cgaaattgcc gcagttcttc
      481 ttcgtttagc ttcttttgct gatgggtgat atttcgccct tcacccgcct cgcctaaccc
      541 gtatcccttg atcgtttttg ccagaatcac ggtcggcgag cctttgtgtt ccacagccgc
      601 cttgaaggca gcataaacct tcaccgggtc gtgccccccg cgcttgagcc gccgaatctg
      661 ttcgtccgtc atgtcggcta cgagggcctc cagccgggga tcaacaccga aaaagtgctt
      721 gcggatatag cttcccggca tgacggtata tttttgatac tggccatcga cgacctcgtt
      781 catgcggcgg accagaagcc catctttatc cttggccagc agcgggtccc aatcagagcc
      841 ccaaataact ttaatgacgt tccagcccgc cccgcgaaag gccccttcca gttcctggat
      901 aatcttgcca ttgccccgga ccggtccgtc caaacgttgc aggttgcaat taaccacgaa
      961 gatcaggttg tccaaacgtt cccggccagc tagggtgatg gcccccaggg tctccggttc
     1021 gtcgcattca ccgtctccca caaaacacca gaccttagac tcgcgggctt gaggcaggat
     1081 gccgcggtcg gccagatact ggttgaaccg agcttggtaa atcgccatga tcggccctag
     1141 tcccatggag accgtgggaa attcccagaa atcaggcatc aaccagggat gaggataaga
     1201 ggacaggcca ccgccttcgg ccaattcgcg acggaagttc tcgagatgct tttcggtgat
     1261 tcggccttcg agatatgccc gcgcataaat cccaggtgcg gcgtgtccct ggatgtaaac
     1321 aaagtcggga ggattaggac cttgcttgcc acggaagaag t
//
LOCUS       DAOPTR010001101         1361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_75816_length_1361_cov_3.106432, whole genome shotgun sequence.
ACCESSION   DAOPTR010001101 DAOPTR010000000
VERSION     DAOPTR010001101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75816_length_1361_cov_3.106432"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PKI05_16050"
     CDS             complement(<1..183)
                     /locus_tag="PKI05_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53765.1"
                     /translation="MGADADSLPVWRGDWWLWLRTRIPFPKGKGSGGDHNTISRLSSA
                     RLLPVLSAYVAWQSWSN"
     gene            complement(1007..>1361)
                     /locus_tag="PKI05_16055"
     CDS             complement(1007..>1361)
                     /locus_tag="PKI05_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53766.1"
                     /translation="GGLLILPEPWARYADIIMHRGPTQYAKLFLALWCLLFLLINLDF
                     CPFSWLTVLAVGTSLRRTLRECQHPFTAPAPFCIAQLLTKGLPKGHTSSLVQGRDLRF
                     NEHSLGILECASTSQ"
BASE COUNT          330 a          365 c          364 g          302 t
ORIGIN      
        1 attcgaccaa ctctgccaag ccacgtaggc actcaagact ggcaaaagtc gagctgagga
       61 caagcgcgaa atggtgttat gatcaccgcc actccccttg ccttttggaa aagggatccg
      121 ggtccgcaac cacaaccacc agtcccctcg ccaaacggga agggagtccg cgtcagctcc
      181 cacttctccc ctctcccttt gggagagggg ttgggggtga gggctatcgc acaaccggta
      241 ctggactatt ggcccccgcc tcgccggggt tttcggacgg gccacattct gcagcgatgc
      301 ctgtgggcct cggcagtggg attgtccctc acccggctcg cggtactcgc caccctcgct
      361 ccgagagaaa tggccagggt tagggcgacc gcacaaattg cgatgcaccc ctaaccccgt
      421 aatcaacggc ttaatcgatg gcgttttgga gtgaggacag ggttatcatg cggaccgctc
      481 agttgttgag gatcggagtc tgcgctcgat gagtccccaa aagcccgtac gaggcccaag
      541 gggtgttatg gtctatgcga caagatgtga taaaccttgc ggagtgacaa cgaccaggtg
      601 gccggcttga tgataaagct ggtgtgcgtt cggattggct acgaaaagct tagcacgcct
      661 tttctagcga tgatccctct cgcctttatc gggcacaata atcacattgt acgcaagaaa
      721 acgctcctgc ggccgacagg gtgcgccaat caatgatcaa cgcatggtaa ctactgtaga
      781 acattccacg ctactaatag gcaatcggta cgcaaatcgc ggcaagatga tctcctttag
      841 tttgtcatta ctatttagcg tctaactctc ttactcttcg tcaaatagcg aaggaaacgg
      901 cacgccatac tttcggctta aattttgacg tgcagtgctt tccgctacat tcaacctcac
      961 ttgagttgat tccaacttat tcatcgcctc ggtaaccaaa tttgggctac tgcgatgtgc
     1021 tagcacattc aagaattcca agactgtgtt cattaaatcg caaatcgcgt ccttgcacta
     1081 agctcgacgt atgccctttt gggagacctt tcgtgagcag ttgggctata caaaagggag
     1141 ccggggcggt gaatgggtgc tgacactcgc gcaatgtgcg tcgcagggag gttcccacgg
     1201 ccagtaccgt aagccaggaa aaagggcaaa agtcgaggtt aataagaaga aagagcagac
     1261 accatagcgc caaaaagagt ttggcatact gcgttggtcc gcggtgcatg ataatgtcgg
     1321 cgtaacgagc ccagggttca ggcaagatca gcaatccacc t
//
LOCUS       DAOPTR010001102         1361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_75819_length_1361_cov_2.968606, whole genome shotgun sequence.
ACCESSION   DAOPTR010001102 DAOPTR010000000
VERSION     DAOPTR010001102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75819_length_1361_cov_2.968606"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..981
                     /locus_tag="PKI05_16060"
     CDS             154..981
                     /locus_tag="PKI05_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOA53767.1"
                     /translation="MRFAICNETFNDRSVEAGFELAAECGYEGIEIAPFTLAAYVTEL
                     TPQRRTEIRRSIEKCGLTTVGLHWLLAKTEGFHLTSPDPAVRARTLEYLKALAECCAD
                     LGGRILVFGSPQQRNVLPETNRLQAEAFAIEVLEGLLPTLERTGTIVALEPLGPQETN
                     FVNTAEEAVAIIKKLSSPHIQLHLDCKAMASETIPIPDLIRTNKQYLVHFHANDPNRL
                     GPGFGELDFVPIFKALLDAGYQGWVSVEVFDYQPGCERIARESIAYMQRCLTEAQKR"
     gene            complement(1086..>1361)
                     /locus_tag="PKI05_16065"
     CDS             complement(1086..>1361)
                     /locus_tag="PKI05_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53768.1"
                     /translation="DENNTTPPDKMYAPDEIDPACKSCHKNHNASAREVVEVYLERCR
                     EITDPKKLTCTNCHGRHRMYHRTIVWDKRTGKLITPTESTEDPHQSP"
BASE COUNT          291 a          343 c          399 g          328 t
ORIGIN      
        1 cgtgacagag cttggccacc cgaccgactc ccgatgggcc attgtcggaa tgaccacggc
       61 tctgaaaaaa gtggaacaga tagacttggt tgtgcttgcc acaaggacat tgaggcttga
      121 ccggcataga tgtcattcga aagggacacg ggtatgagat ttgcaatttg caatgaaacc
      181 tttaatgatc gctctgtaga agcgggattc gaattggctg cagaatgcgg atacgaggga
      241 atagagattg ccccttttac gttagccgcc tatgtcacgg agttgacacc ccagcgccga
      301 acggaaatcc ggcgttcgat cgagaaatgc ggcctgacga ccgtgggact gcattggctg
      361 ctggcaaaaa cagaagggtt tcatttgact agccctgatc ccgctgtccg ggcgagaacc
      421 ctggagtatt tgaaggcctt ggctgaatgc tgtgccgatt tgggcggtcg catcctggtt
      481 ttcgggtcgc ctcaacagcg gaatgtcctg ccagagacaa accgccttca ggcagaggcc
      541 tttgcgattg aggtcttgga aggactcctc cccacattgg agcgaaccgg tactattgtg
      601 gcactggagc cccttggccc gcaggagacc aattttgtta atacggccga agaagctgtg
      661 gcgattatta agaaattgtc ctcgccgcac attcaacttc atctggattg caaggcgatg
      721 gccagcgaga ccatcccaat acctgatctg atccgtacca acaaacagta tttggtgcat
      781 ttccacgcca acgaccccaa tcggctcggc ccgggatttg gcgaattgga ttttgtaccg
      841 atctttaagg ccttgctcga cgcgggctat cagggctggg tctccgttga ggtcttcgac
      901 taccaacctg gctgtgaacg gatcgcccgg gaaagtattg cctatatgca acgatgtttg
      961 accgaggctc aaaagcgcta ggtggcacga ggccaccctg gcgctgcgtc aaagacgtac
     1021 tgagcgcatt ctgggacggg tcttcggcga atgacatttc gctatcggcc gatcgtgtgt
     1081 aggggttaag gcgattgatg aggatcttcg gtagactccg tcggcgtaat cagtttgccg
     1141 gtgcgcttgt cccacacgat ggttcggtgg tacatgcggt ggcgcccatg acagttggta
     1201 caggtgagct ttttgggatc ggtgatctct cggcaacgtt ctaggtaaac ctcgaccact
     1261 tcccgggcgg atgcgttgtg atttttgtgg cagcttttac acgccggatc gatctcgtcc
     1321 ggggcgtaca tcttgtccgg cggggtcgta ttgttttcat c
//
LOCUS       DAOPTR010001103         1357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_76150_length_1357_cov_2.947005, whole genome shotgun sequence.
ACCESSION   DAOPTR010001103 DAOPTR010000000
VERSION     DAOPTR010001103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76150_length_1357_cov_2.947005"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PKI05_16070"
     CDS             complement(<1..425)
                     /locus_tag="PKI05_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53769.1"
                     /translation="MSSFNLSNLWNFGLQPRPMFRLRMPQNRAAPVEVRPVRTWREQY
                     YFTRLPWLIYQNDPAWVPPLLIEVKEFLNPRKHPFLLHGQATQFLACQDGCPVGRILV
                     SVDPRYNAKHGTKVGCFGLFESINDVRVAHALFSAAAEWL"
     gene            complement(490..>1357)
                     /locus_tag="PKI05_16075"
     CDS             complement(490..>1357)
                     /locus_tag="PKI05_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOA53770.1"
                     /translation="RRGFFGFFESVDDERVAHGLFDAARQWLASHGLETLRGPTNPSL
                     NYELGLLIEGFDSPPTFMMTYNPPYYQRLIESYGFRKTQDLYAYWGHVSMLPEIRAKL
                     KPISDKIVERYNVKIRMLDKRRFLEDVELFLNIYNRSLINTWGFVPMTPAEVRYMAKG
                     LRWLMVPELALGVEVDGRMVGAVFCLPDYNQRIKEINGRLFPFGFIHLLRNKHAIKRV
                     RVISTNVVPEYQLMGFGLVLLDGLVPKALEWGIEEAEFSWVLESNALSRGSLEKGGAK
                     RTKTYRLYDYGG"
BASE COUNT          326 a          381 c          349 g          301 t
ORIGIN      
        1 agccattcgg cggcagccga aaagagggca tgcgccaccc tcacgtcgtt gatcgattca
       61 aatagtccaa aacagcccac cttcgtcccg tgtttcgcat tgtaacgagg atcaacactc
      121 acaaggatgc gtcccacggg acacccgtcc tgacaggcca ggaattgggt cgcctgacca
      181 tggagaagaa acggatgctt gcgcgggttg aggaactctt tgacctcgat aaggagcggc
      241 ggaacccagg ccgggtcgtt ctgatagatc agccacggca gtcgcgtgaa atagtattgc
      301 tcgcgccagg tacggacagg tcgcacctcc accggggctg ctcgattctg tggcatccgc
      361 agccggaaca ttggccgtgg ttgcaggccg aaattccaga gattcgaaag attgaacgat
      421 gacatcgtta tatgaatccg ggccggattg tcagtcaatc acgtatgcct ttagccatgc
      481 cacagatgct catccgccgt agtcgtacaa ccgatacgtt tttgttcgct ttgccccccc
      541 tttttcgagg ctccctcggg aaagagcatt tgactccaag acccaagaga attccgcttc
      601 ttcgattccc cattccaaag cttttggaac caatccgtcc aaaagaacca gcccaaatcc
      661 catcaattgg tattcgggca caacgttagt gctgatgacg cgaacacgtt taatggcgtg
      721 cttgttcctc aaaaggtgga taaaaccaaa aggaaacaag cgaccattga tttctttgat
      781 ccgctggttg tagtcgggca ggcagaagac ggcacccacc atgcggccat cgacttctac
      841 gcccagcgcc agttcaggca ccatcaacca gcggagtccc ttcgccatgt aacggacctc
      901 cgcaggagtc atggggacaa acccccatgt gttgatcagc gatcggttat agatgttgag
      961 aaatagttca acgtcttcca ggaatctcct tttgtccaac atgcggattt tgacgttata
     1021 tcgctccaca atcttgtcgc tgatgggctt cagttttgcc cgaatctctg ggagcatact
     1081 gacatgcccc cagtaggcgt aaaggtcttg cgtctttcga aaaccgtagg actcgatcag
     1141 ccgctgatag tagggcggat tgtaagtcat cataaaggtc ggcggcgagt cgaagccttc
     1201 gatcagcaaa cccagttcgt agttgagact gggattggtc ggtccccgta gcgtttccag
     1261 gccgtgactg gcgagccatt gccgcgcggc atcgaacagg ccgtgtgcca cccgttcatc
     1321 gtccacagac tcaaagaacc caaaaaaacc tcgccgt
//
LOCUS       DAOPTR010001104         1356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_76237_length_1356_cov_2.763259, whole genome shotgun sequence.
ACCESSION   DAOPTR010001104 DAOPTR010000000
VERSION     DAOPTR010001104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76237_length_1356_cov_2.763259"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="PKI05_16080"
     CDS             <1..780
                     /locus_tag="PKI05_16080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53771.1"
                     /translation="RLLAGQTWDVISPLLPGTIMYSVGWGAGNLGYRRAQFRAERYLH
                     FSDTLLVTTQASINSDVVSEFAGNVIGDHADWPVVEGRLAFTLGPRGKGCRPVEIGIS
                     SHIGEQVYDFLAAPVQDDVEFRTWSFNVDAKWPITSRFGVQGEFFTGENLGAYLGGIV
                     QGINLTQRDTIRSTGGWFDIWYDWTSRLHTHAGYSIDDPLNGGLSPGQRSYNQFYYVN
                     LTYDVTKKFVLGIEVQQWKTNWVNKEPGDSYRLELMAKYGF"
     gene            complement(859..>1356)
                     /locus_tag="PKI05_16085"
     CDS             complement(859..>1356)
                     /locus_tag="PKI05_16085"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOA53772.1"
                     /translation="AMMVLDTIMSGYDYPGGWLHEELRGEGLVYGVHALQLTGPVPGY
                     FVIIAQTRPEAQNEVITRILNNVDKAKRGQISEEEFLRAKELLVAYHAQKNTTIEEQA
                     QQAILDELYGLGFDYDRGFETRIQAITLDDVKRVAREYFGNYVLATVSPLKSLPEEAS
                     TGGDR"
BASE COUNT          287 a          382 c          362 g          325 t
ORIGIN      
        1 cgtttgctcg ccgggcaaac ttgggacgtg atctcgcctc tactgccggg gacgatcatg
       61 tactcggtcg gttggggagc gggtaacctt gggtaccggc gtgctcaatt tcgggcagaa
      121 cgctatctcc acttttctga taccctgtta gtaaccaccc aggcctcgat caatagcgat
      181 gtcgtttccg agttcgccgg taacgttatc ggggaccacg cagactggcc tgtggtcgag
      241 ggacgactgg ccttcacgct cggcccgcga ggcaaagggt gccgtcccgt ggaaatcggt
      301 atttccagcc atatcggcga acaggtttat gattttctcg cagcacctgt acaggatgac
      361 gttgagttcc gcacttggtc gttcaacgtg gacgccaaat ggccaatcac ctcccgcttt
      421 ggtgtccagg gcgaattctt taccggcgaa aaccttggcg cctacttggg tgggatcgtt
      481 cagggaatta acctgacgca acgagacacc atccgttcca ccggtggatg gttcgacatc
      541 tggtacgact ggaccagccg actccatacc cacgctgggt attccatcga cgacccgctc
      601 aatgggggcc ttagtcccgg gcaacggagc tacaaccaat tttactatgt caacctaacc
      661 tacgatgtca ctaagaaatt tgtcctgggt atagaagttc agcaatggaa gacgaactgg
      721 gttaacaagg aacccggtga cagttatcgc ttggagctga tggccaaata cggattctga
      781 caacccgggc gaatggccgt tttcggcaac cgtgggaagg catcccgcct catcccacgg
      841 ttgctccttt ttttgcgatc aacgatcgcc acccgttgag gcttcttccg ggagagactt
      901 cagaggcgag accgtggcaa ggacgtaatt gccgaaatac tcccgtgcaa cccttttgac
      961 atcgtcgagt gtgatcgcct ggatgcgagt ctcgaacccc cggtcgtaat cgaaaccaag
     1021 cccgtacaac tcgtcgagga tggcctgttg ggcctgttct tcgatggtcg tgtttttctg
     1081 ggcatgatag gccactaaaa gctctttggc ccgtaagaac tcttcctcgc tgatctgtcc
     1141 gcgttttgcc ttatcgacat tgttaagaat ccgcgtgatg acctcgtttt gggcctctgg
     1201 cctcgtttga gcaatgatca caaaataacc tgggacgggc ccggtcagct gtagggcatg
     1261 gaccccatac actaatcctt cccctcgtag ttcctcatgt agccagccac ccggatagtc
     1321 atagcccgac ataattgtgt caaggaccat catcgc
//
LOCUS       DAOPTR010001105         1355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_76308_length_1355_cov_3.348462, whole genome shotgun sequence.
ACCESSION   DAOPTR010001105 DAOPTR010000000
VERSION     DAOPTR010001105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76308_length_1355_cov_3.348462"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="PKI05_16090"
     CDS             complement(<1..1051)
                     /locus_tag="PKI05_16090"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOA53773.1"
                     /translation="MTKLPGDRHSSATAACVSQVSSSLWSRSFLALLVTQFTVALNDN
                     ILRWLFIPIGKDLVLEQLKESPDVARSVGSFIFLLPFILLAGWAGATSDRFSKRTVII
                     GAKVAEIIVMTLAIVAILTSNVWAMAVVLFLAGTHSAFFSPSKYGSIPEIVGPKLISA
                     ANGVIGLTTMIAVIVGGSVGNLLYSLTTIRDPALAQARGLGPGQYRWWINAVVLLGVA
                     TLGWLTSLAIKRLPAADPQRRIPLNPFLQCYYDLKELSRHRVLLLAAIGSTYFWTLGL
                     LMQLTIDKLAVPELVGPYGQAYVGPMLGILTLGIGLGSMLAGVWSHGRIERGLVPLGA
                     LGITVISLAFAFLPAG"
BASE COUNT          342 a          352 c          391 g          270 t
ORIGIN      
        1 tcccggcggg aaggaatgca aaggcgagag aaatcacagt gatgcccagg gcccctaaag
       61 ggacgagtcc ccgttcgatc cgcccgtgag accacacgcc cgcaagcata ctgcccaacc
      121 caatgccaag cgtcaggatg cctagcatcg gtccgacgta tgcctggcca tagggcccca
      181 caagttcggg tacggccagt ttatcgatcg taagctgcat taacaggccc agggtccaaa
      241 aataggtact tcctatggcg gctaggagca gcactcgatg gcgagataac tccttgaggt
      301 cgtagtaaca ctgtaggaag ggattcaggg gaatgcgtcg ttgagggtca gcggcgggca
      361 gacgtttgat cgcaaggctt gtcagccatc ctaaggtggc cacgcccaag aggacaacgg
      421 cattgatcca ccagcgatat tgacctggtc caagcccacg cgcttgggca agtgccggat
      481 cgcgaattgt cgtgagactg tataagagat ttcccaccga cccgcccacg atcacagcga
      541 tcatcgtagt cagaccgatg acaccgttgg cagccgagat gagtttcggt ccgacaattt
      601 cgggaatgct gccgtatttc gagggactga aaaaagcgct gtgtgttccg gccaggaaga
      661 ggactaccgc catggcccag acgttactgg tcaaaatggc gacgatggcc aaggtcatca
      721 cgataatctc ggcaactttc gcaccgatga tgacggtccg tttgctgaac cgatcagaag
      781 tggccccagc ccagcctgcc agcagaataa agggcaaaag gaaaatgaac gaccccaccg
      841 atcgggcaac gtcagggctt tccttcaact gttcaaggac gaggtctttg ccgattggga
      901 taaacaacca ccgcaaaatg ttgtcgttga gagcaacggt gaactgcgtc actaagagcg
      961 cgagaaaact acgtgaccaa agacttgacg acacttgcga tacacaggct gcggtcgcgc
     1021 tagaatgtcg gtcccccggc agtttggtca tttacggctc cataggatag caaagtgagg
     1081 acattcgggt ttcattttcg ttctatggga aagcatgcag tttagggaac tgtcccattt
     1141 ggatagcgta gtacctgccc tgagttttga caatttgtct tgggcagatt gcctctttgg
     1201 acttaaacgc ggacgatagc cgcaacaagt gtcatgagaa agagcgggaa gtggcgcact
     1261 gggggatggg tccgcccact cccgaaagag taccccaggt tatacaatgc gggttttggg
     1321 ccagacacgg gaatgttcgg cctgaggact atcgt
//
LOCUS       DAOPTR010001106         1354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_76381_length_1354_cov_4.407236, whole genome shotgun sequence.
ACCESSION   DAOPTR010001106 DAOPTR010000000
VERSION     DAOPTR010001106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76381_length_1354_cov_4.407236"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PKI05_16095"
     CDS             <1..479
                     /locus_tag="PKI05_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008659665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA53774.1"
                     /translation="KGMTRRIGLAQALINDPDLILLDEPTSGLDPIGTREMKDLILRL
                     RDQGKTIVMCSHLLADVQDVCDRIGILYQGELKELGRVDQLLALKDITEIRVPRMSPD
                     AEQEVKAVLRKHGLEVLAWGNPTTTLEDLFLRIIRESEEHPGLRVHGRGDGHADRN"
     gene            463..>1354
                     /locus_tag="PKI05_16100"
     CDS             463..>1354
                     /locus_tag="PKI05_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA53775.1"
                     /translation="MPIETDLPEFSTWILPALERWLLAGLGLLTLCCFVGLLWCLLLY
                     RRHGWRRFGDLLVGGFLDLVRVNGRRIGALAWLTVKEALGRRVTVVVLIFLLGLLFAG
                     WFLDPASTEPHRLYLNFVLGTTNYLVLLLMVFLTAFRLPSDIQNKTIYTVLTKPVRTV
                     EIVTGQVLGFIVVGSILLGIMWIVSYPFVVRGLYHTHQVNVDELRPLGEPVTLPDGTT
                     ATALQGWTASTWQHRHRFFVDANGNPRVDRERGHYHNVTVVRSADGKTSYVLGGPEGQ
                     LVARVPVYAKKLTFRDRGGLD"
BASE COUNT          252 a          367 c          412 g          323 t
ORIGIN      
        1 cgaaagggat gacacgccgc atcggcctgg cccaagccct catcaatgat cccgatttga
       61 tcctactgga cgagccgacc tccgggcttg acccaatcgg gacgcgggag atgaaggatc
      121 ttatccttcg cctgcgcgac caaggcaaga cgattgtcat gtgcagccac ctgttggcag
      181 acgtgcaaga cgtctgtgac cggatcggga tcctttatca gggagaactc aaggaactgg
      241 ggcgggtcga tcaactcttg gccctgaagg acattacgga aatacgcgta ccgcgaatgt
      301 cacccgacgc cgagcaggag gtcaaggctg tcttgaggaa acacggctta gaggtactgg
      361 cctggggcaa tcctacgacg acgttggagg atctcttctt gcgaatcatt cgcgagagcg
      421 aagagcatcc gggacttcgg gtacatggtc gaggtgacgg gcatgccgat cgaaactgat
      481 ctcccagaat tctccacatg gattctcccg gcccttgaac gatggttgtt agctgggctc
      541 gggttgttga ctctgtgctg cttcgtcggc cttttgtggt gcctccttct ttaccgtagg
      601 cacggttggc ggcgattcgg ggatttgttg gtggggggct ttctcgatct tgtccgggtc
      661 aacgggcgac gcatcggtgc gctggcctgg ttaactgtca aagaggccct ggggcggcgg
      721 gtgacggtgg tcgttttgat tttcctcctc gggctgctgt ttgcgggatg gttcctcgat
      781 cccgccagca ccgagccgca ccgactttat ctcaacttcg tgttgggcac gaccaattac
      841 ttggtccttt tgttgatggt cttcctcacc gcattccggc ttcccagtga catccaaaac
      901 aagacgattt acacggttct taccaagccg gtccgaacag ttgagatcgt caccgggcag
      961 gtgttggggt tcatcgtggt gggaagtatc ctgctcggca tcatgtggat tgtcagttac
     1021 cccttcgtgg tgcggggcct ttatcatacg catcaggtca acgtggatga attgcggcct
     1081 cttggggagc cggtgacgct gcccgacggc accacggcca cagccttgca aggatggacg
     1141 gcttccacct ggcaacaccg ccatcgcttt tttgtggatg ccaacggtaa tccccgggtc
     1201 gatcgggagc gtggccacta ccacaacgtg accgtggttc ggtcggctga tggtaagacc
     1261 tcgtatgttc taggtggtcc tgaaggtcaa ctcgttgccc gtgtgccggt ttatgccaaa
     1321 aaactgacgt tccgcgatcg cggtggcctt gata
//
LOCUS       DAOPTR010001107         1354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_76385_length_1354_cov_3.881447, whole genome shotgun sequence.
ACCESSION   DAOPTR010001107 DAOPTR010000000
VERSION     DAOPTR010001107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76385_length_1354_cov_3.881447"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..1262
                     /locus_tag="PKI05_16105"
     CDS             189..1262
                     /locus_tag="PKI05_16105"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOA53776.1"
                     /translation="MASSQDMMKSSDSTESLKPSRRDYLAMLSAGAGLGLASLLGCRP
                     ETVSQTMGQQRRLRAAFSNAGLAGTWNKLGYDAAKLWGDLLNVEVVWFDGEFNGQRQR
                     EKIELAAAEDWDFCAFQAHQIDILAEPCRRLKARGIPIIGMDTLIVAEDKLFDHGVVV
                     YVTPDHVRMAEQSTQYMVDKIGGKGKVIHIGGQSEHSGAQDRAKGFQNIISKYPEIEV
                     VGGGVRWCDWKTEKARDTFDVLLEQTPDRIAGAFFHNDDMALACVPRLKGTRHEGMVV
                     TGVDGQRPGLLGVRDGLLAATSVNPAAMIHMYALVIGQFIVRNQEKFDKLLKIVLPCP
                     LVSQESGNLDAMLYLSDPKHCLV"
BASE COUNT          292 a          383 c          396 g          283 t
ORIGIN      
        1 tgttggctat tgcactccag ccgccccgag aacacagatc gtagtccgcc ttcaatccgg
       61 cttggggccc accacttcgc caaacagggc cgcatcgcat tggttgattt tttggcgtga
      121 tccattacag tgatagacgc gccctgcgtc acactgtggt gggaggacgt cagcgcttgg
      181 aggtcaatat ggcaagttcc caggacatga tgaagtcttc cgattcgacc gaatctctca
      241 aaccatcgcg tcgcgactat ttggcgatgc tctctgcggg ggcggggctg gggttggcgt
      301 cgcttctggg ttgccgaccc gagacggtct cccagacgat gggccagcag cggcgattac
      361 gagcggcctt ttccaatgct ggtttggccg gcacctggaa caagttaggc tacgatgccg
      421 ccaagctatg gggcgatcta ctcaatgtcg aagtcgtttg gttcgatggg gaattcaacg
      481 gtcaaagaca gcgtgaaaag atcgaacttg ccgctgctga agactgggac ttctgtgcct
      541 ttcaggctca ccaaatcgac atcctagccg agccatgccg acgccttaaa gcccgcggca
      601 tccccatcat cggcatggat acgctcatcg tggccgagga taaactcttc gatcatggcg
      661 tggtcgttta tgtcacgccc gatcacgtcc ggatggctga acagagcacg cagtacatgg
      721 tggataaaat cggcggcaag ggcaaagtca tccatattgg cggtcaaagt gagcacagcg
      781 gggcccaaga tcgcgccaaa ggttttcaga acattattag caagtaccca gagatcgaag
      841 tcgtcggtgg tggtgtccgt tggtgcgatt ggaagaccga aaaggcccgc gataccttcg
      901 acgttcttct cgagcaaacc ccggaccgca tcgcgggggc tttctttcac aatgacgaca
      961 tggccctcgc ttgcgtccca cgactcaaag ggactcgtca tgagggaatg gtcgtgaccg
     1021 gcgtcgatgg ccagcgccca gggttgttgg gcgtgcggga cggcttgcta gccgctacga
     1081 gcgtcaatcc cgcggccatg atccacatgt atgccctagt catcggtcag tttatcgtcc
     1141 gcaatcagga gaaattcgac aagctgctca agattgtcct gccgtgtcct cttgtctcgc
     1201 aggaatcggg caacctggac gcgatgctgt acctctccga tcccaaacat tgcctcgttt
     1261 aacaagccaa gcacgaaatg gcagacgcgg cggacgccat ggaccgcgtc cctccccagc
     1321 ggtccaaggg gaaaaagatc ggaggcggag gacc
//
LOCUS       DAOPTR010001108         1348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_76879_length_1348_cov_3.186388, whole genome shotgun sequence.
ACCESSION   DAOPTR010001108 DAOPTR010000000
VERSION     DAOPTR010001108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76879_length_1348_cov_3.186388"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PKI05_16110"
     CDS             complement(<1..538)
                     /locus_tag="PKI05_16110"
                     /inference="COORDINATES: protein motif:HMM:NF014027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HOA53777.1"
                     /translation="MQDNCGNKKDPLHDKLLGGRRILPPHVGSCETVADLIDRVFLAY
                     NAARLREAAQLFTRKMLNDDVTIGMSLAGALTPAGLGMSCVVPLIEAGFVDWIVATGA
                     NLYHDLHFAYNYKLVAGDFRLDDRQLHEVGVVRIYDVLLSLDECLIETDRRVQEMLSQ
                     PEFQRSMTTAELHYLMGRY"
     gene            863..>1348
                     /locus_tag="PKI05_16115"
     CDS             863..>1348
                     /locus_tag="PKI05_16115"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOA53778.1"
                     /translation="MLRKIVLGLSLVFLTFCPVLAQEKASAAKPRNIILIGWDGAQRA
                     HVMECLERGELPNLKQLSQEGALVNIDVVTGATDTKAGWTQILTGYNPEVTGVYSNGR
                     YRDVPKGLSIFERLKEHFGSDFVCVAVIGKREHCGEIREPFKRQLTDEEAAKIQAQQE
                     AA"
BASE COUNT          312 a          340 c          381 g          315 t
ORIGIN      
        1 cataccggcc catgaggtaa tgcaattctg ccgttgtcat ggaccgctgg aattctggtt
       61 gggacagcat ttcctggacc cgtcggtcag tttcgatgag gcattcgtcc aaagacagga
      121 ggacatcgta aattcggacc acgcccacct cgtggagctg gcgatcgtca agccgaaagt
      181 cgcccgccac gagcttgtag ttgtaggcaa agtgcaaatc gtggtacaaa ttagcgccgg
      241 tggccacaat ccaatccaca aatccggctt caataagagg aacgacacat gacatcccta
      301 atccggcggg tgttaatgcc cctgccagtg acatgccgat cgtaacatcg tcgttcaaca
      361 tctttcttgt gaacaattgc gcggcttcgc gtaagcgggc ggcattgtag gccaaaaaga
      421 cgcgatcaat caggtcagca acggtttcgc atgatcccac atgagggggg agaatcctcc
      481 ttccaccaag gagcttatcg tgaagcgggt ctttcttgtt tccgcaattg tcctgcatcg
      541 cttcaacctc tacctctcaa taagagtaag cctgtttcgc tgtaaaaagt ttcgcgtcgt
      601 gcttgacggg taccgtcttc cgattgaggc atccgctgtg gatagcacat gttggaaacg
      661 ttttatttaa ccaggattcg ggatcgaatc caagccgcga gtgattcaaa taatcccttt
      721 ttagcgtgca tgtgacccca ctccgcgcaa ttcagttacc gagggaatca cgctccggga
      781 gggtacctgc ctcctggagt ggtggcttgt cagtgttgtt ttttcgatgg cgtttccaag
      841 agatcgcgga ggagtggtgg ccatgttacg caaaattgtt ttgggtttga gcctggtgtt
      901 tttgaccttt tgtccggtcc tcgcacaaga aaaggcctcg gccgcgaaac caaggaacat
      961 catcctcatt ggctgggatg gtgcccaacg ggcccacgtc atggagtgtt tggagcgcgg
     1021 tgagttgccc aacttaaagc aactttcaca agaaggggcg ctggttaata tcgacgttgt
     1081 gacgggggca accgatacaa aggcggggtg gacacagatt ctgacgggct ataacccgga
     1141 ggtcacaggc gtttacagca acggtcgcta ccgggatgtg cccaagggcc tgagcatctt
     1201 tgaacgactc aaggaacact ttgggtccga ttttgtttgt gtggcggtga tcggcaagag
     1261 agagcactgc ggcgagattc gcgagccgtt caagcggcag ctcacagatg aagaggccgc
     1321 taagattcaa gcccagcaag aggcggcc
//
LOCUS       DAOPTR010001109         1343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_77289_length_1343_cov_4.306677, whole genome shotgun sequence.
ACCESSION   DAOPTR010001109 DAOPTR010000000
VERSION     DAOPTR010001109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77289_length_1343_cov_4.306677"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(370..831)
                     /locus_tag="PKI05_16120"
     CDS             complement(370..831)
                     /locus_tag="PKI05_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOA53779.1"
                     /translation="MRMKAFRTSFLVFSVVVLPFVATGCGEKGPKTVQGTGVVTYKGQ
                     PVEGANVMFSPITQGQGVAALGKTDAAGKFTLQTVQGKAGAVPGEYAVVITKTEMVPT
                     GEKVPKPEGGTEDVMINKDLLPQKYKFASSTPLKVKIEEGKVNEFQFNLED"
     gene            complement(903..>1343)
                     /locus_tag="PKI05_16125"
     CDS             complement(903..>1343)
                     /locus_tag="PKI05_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOA53780.1"
                     /translation="SYAGDVDIKSGIGTSVNPVRQPPAVCLNLRTAQNTISGSTSSGK
                     ARRWADSRNYYGVFFTCLPPNTVSCGPERDALITASSYHPGGVNAAFVDGSVRFISET
                     IDAGDPTKTLPAPNPSEPQHYTGPSIYGVWGALGTRACNDTASL"
BASE COUNT          305 a          406 c          344 g          288 t
ORIGIN      
        1 aagccccttc gccttccgac acgacatacc cgatggacca tgccccggcg accgctctta
       61 ttgtagccgt ccccaaattg tgagcaagaa cgcaaaggtc ctcctaaacg ggtgcgcttg
      121 ttcccagcag tgtcgatccc gtcctaccag tccgagatcg ctgaagggaa tttgcgaagg
      181 ttgcacaagc gagggaaatg tcccccgcgc agacgcttcg gcagggatcg acacagattt
      241 gaggggcgat ttcaacgcgg gaggaggcgt acctctgggg cggattctcg gattgggccc
      301 aataaagaaa ccgcctgaaa ccgtccaggg ttccaggcgg tacacagtca aaggcggccg
      361 aaatggtact tagtcctcaa gattgaactg gaattcgttc actttccctt cctcaatttt
      421 taccttcaaa ggtgtggagg aggcaaactt gtacttttgt ggtaaaaggt ccttgttgat
      481 catgacgtct tccgtgccgc cttcgggttt aggcaccttt tcgcctgtgg gtaccatttc
      541 cgtcttggtg atcacgacgg cgtactcacc gggaacagct ccagctttcc cctgcaccgt
      601 ctgaagcgtg aacttcccgg ctgcgtcggt cttgcccaag gccgctacgc cctgcccctg
      661 agttatcggg ctaaacatca cgttcgcgcc ttccaccggc tggcctttat aagtgacgac
      721 acctgttcct tggaccgtct tggggccttt ttcgccgcag cccgtcgcaa caaaaggcaa
      781 gaccactact gaaaatacaa gaaaactcgt ccggaacgct ttcatcctca taatcactat
      841 cctgcctcaa aaaatctctc gctattccgt cttcccaaaa cgcgccacgc aaacaccggg
      901 gcctacagag aagccgtgtc gttgcaagcg cgcgtcccaa gagcccccca cacaccataa
      961 atggacgggc cggtataatg ctgtggctcg ctcggatttg gggcgggtaa cgttttggtc
     1021 ggatccccag cgtcaatggt ctcgctgata aaacgcacag aaccgtcaac aaacgcggca
     1081 ttgaccccac ccgggtgata gctcgaagcg gtgatcaacg cgtctcgttc cggcccacaa
     1141 gaaaccgtat tcggcggcaa acacgtaaag aacacaccgt aataattacg agagtccgcc
     1201 caacgacggg ccttgcccga cgatgtactt ccggaaatcg tgttctgagc cgttcgcaga
     1261 ttcaggcata ccgcgggtgg ctggcgaacc ggattgacgc ttgtgccaat ccccgacttg
     1321 atatcaacat ccccggcata gga
//
LOCUS       DAOPTR010001110         1340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_77582_length_1340_cov_3.371984, whole genome shotgun sequence.
ACCESSION   DAOPTR010001110 DAOPTR010000000
VERSION     DAOPTR010001110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77582_length_1340_cov_3.371984"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..>1340)
                     /locus_tag="PKI05_16130"
     CDS             complement(67..>1340)
                     /locus_tag="PKI05_16130"
                     /inference="COORDINATES: protein motif:HMM:NF013882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HOA53781.1"
                     /translation="ARLKQYGWPFKQPIVYPAIEGDKVGVPPEFGQGGGAYGCRMSHL
                     RILQDCLMDDVKTVMILEDDAEITEGFPERVREFLAKVPDDWEGIMLGGQHHAPPIPT
                     GIDGVVRVRYAQRTHCYIARPSYMKALQRRWGNATVHIDWRMRDWQHQFVVYAPDPWL
                     VGQAGGRSDIRGAEKPPEWWISGSHLPPGPVAVAKVPRDVLDGLVFRGFHPGWTRDND
                     GIDEGLHRVFRNPPGSARAQALRKWLEMIQSECVGGLLPTVYHHDATLELVQEAWSGP
                     VHLVTGDSVESAWSCLPPDIREMMRTKQTLRKARVVLLRCPRVMIDQLHEHGFHPGYW
                     RDRETGLDMGLKRIFEGVEESKRIDELRKWCEWLLREAKRDGLIVTAIHPKLTLEDLK
                     AASPFDVIEVEATSIDQLKQAITGFATLCDR"
BASE COUNT          242 a          430 c          389 g          279 t
ORIGIN      
        1 ccagtaaagc cagagcgtat tttcgccata atgttcaacc atactgatct cctatgcgga
       61 ttctactcag cggtcacaga gcgtcgcgaa tccagtgatt gcctgcttga gttgatcaat
      121 gcttgtcgcc tcgacctcta tgacatcaaa ggggctggct gccttcagat cctccagcgt
      181 gagtttcgga tgaatcgctg tcacaataag cccatcgcgt tttgcctccc gcaggagcca
      241 ctcgcaccat ttccgcaact cgtcgatccg cttcgattcc tccacgccct cgaaaattcg
      301 cttcagcccc atatcgagcc cggtttcccg gtcccgccaa tagccagggt gaaagccgtg
      361 ttcgtgaagt tgatcgatca tcacccgggg gcaccgcaag aggaccaccc gggctttgcg
      421 gagtgtttgt ttggtccgca tcatttcgcg aatgtccggc gggaggcaac tccaggcgga
      481 ctccacactg tctccggtga cgaggtggac cgggccagac caggcctctt ggacgagctc
      541 cagggtggca tcgtggtggt acaccgtcgg cagtaacccc ccgacacact cgctctggat
      601 catttcaagc cacttgcgga gggcctgtgc ccgggcgctc cccggcggat tgcgaaacac
      661 ccggtgaagg ccctcgtcga tgccgtcgtt gtcgcgtgtc caacctgggt ggaagccgcg
      721 aaagacaagg ccgtcgagaa cgtcccgcgg taccttggcc accgccaccg ggccaggcgg
      781 gaggtggctc ccactgatcc accactccgg cggtttttcg gccccgcgga tgtcgctccg
      841 gccaccggct tgacccacca gccacggatc gggagcgtac accacaaact gatgttgcca
      901 gtctcgcatt cgccagtcga tatgaacggt cgcgttgccc caacgccgct ggagggcctt
      961 catatagctt ggccgggcga tgtagcagtg cgtcctttgg gcgtagcgga ccctgacaac
     1021 gccatcaatg ccagttggaa tcggtggggc gtggtgctgg cccccaagca tgatcccttc
     1081 ccagtcgtcg gggaccttgg ccagaaactc cctcacccgc tccgggaagc cttcggtgat
     1141 ttccgcgtcg tcctctagga tcatgacggt tttaacgtcg tccatgaggc aatcttggag
     1201 gatgcgaagg tggctcatcc ggcagccgta ggccccgccg ccttgcccga actccggcgg
     1261 cacgccgact ttgtcgcctt caatagccgg atagacgatg ggttgtttga atggccagcc
     1321 gtactgcttg aggcgggcgt
//
LOCUS       DAOPTR010001111         1340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_77606_length_1340_cov_2.442802, whole genome shotgun sequence.
ACCESSION   DAOPTR010001111 DAOPTR010000000
VERSION     DAOPTR010001111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77606_length_1340_cov_2.442802"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..>1340)
                     /gene="cimA"
                     /locus_tag="PKI05_16135"
     CDS             complement(139..>1340)
                     /gene="cimA"
                     /locus_tag="PKI05_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOA53782.1"
                     /translation="EVIFDAEHFFDGYKYDADYALTAVRVAAEAGARTVVLCDTNGGS
                     LPNEIAEMTRKVVDILPVPVGIHCHNDSDVAVANSLAAVDAGATHVQGTINGFGERCG
                     NADLISVVANLRLKKGYDVLLPESLRHLTALSRFVYETVNMPYRNNQPYVGRGAFAHK
                     GGMHVSGVNRTPGSYEHIDPQLVGNERRILVSELSGRSNIMAATAKLNIQADPKILER
                     ILAKVVSREHAGYQYEAAQGSFDLLVKQCLGTFKPHFERLAYRVNIETESDGRIVTEA
                     TVKIRVGNEVRHEVAEGDGPVNALDAAMRKALNGTYPSLASMQLVDYKVRVINSEAGT
                     AAGVRVMIESRDETDSWGTVGVSENIIEASWIALTDSYEYKLCKDEERKAQTKESSNH
                     VPERVTA"
BASE COUNT          269 a          400 c          337 g          334 t
ORIGIN      
        1 acgatcgtgt atggcttccg cgatggatcg ggatcggcat taaagtagcc tttttcttca
       61 cagaactgat accaagtcgc ctcggcaact ttgggatcgt attgtttcgg taggtcggtc
      121 gttcctacca aggtttgttt aggctgtgac tctttctggg acatggtttg aactttcctt
      181 tgtctgtgct ttccgttcct cgtctttgca caacttgtac tcgtaactat ccgtgagggc
      241 aatccagctt gcctcgatga tgttctcgct gacacccacg gtgccccacg aatcggtctc
      301 gtcacggctt tcgatcatga cacgcacgcc ggcggcggtc ccggcctcgg agttgataac
      361 acgcaccttg taatccacaa gctgcatgct ggccaagcta ggatatgtcc cattgagggc
      421 cttccgcatc gccgcgtcaa gggcgtttac ggggccatcc ccctcggcaa cctcgtgccg
      481 gacttcatta ccgacacgaa tcttcacagt ggcctcggtc acaattcgtc catcgctttc
      541 cgtctcgatg ttcacccggt aggccaaccg ctcgaaatgc ggcttaaagg tccccaaaca
      601 ttgcttgacc aaaaggtcga acgaaccctg ggcggcctca tactgatagc ccgcatgttc
      661 acgggaaaca accttggcca ggattcgttc taggattttc ggatcggcct gaatatttaa
      721 ttttgccgtg gccgccatga tatttgagcg ccccgacagc tcgctgacga gaattcgcct
      781 ttcgtttccc actaattggg ggtcaatgtg ctcgtaactc ccgggcgtac gattgacgcc
      841 actcacgtgc atgccccctt tatgggcgaa tgcgcccctg cccacgtagg gctggttatt
      901 tcgatacggc atattgaccg tttcataaac aaagcgggag agcgccgtaa gatgccttaa
      961 tgactccggc aataacacgt cgtatccctt cttcagtcgc aagttggcca caaccgaaat
     1021 caaatcggca ttaccgcacc gttccccaaa gccgttgatc gttccctgga cgtgggtcgc
     1081 gcctgcatcc actgccgcca gcgagttcgc tactgccaca tcactgtcgt tatgacaatg
     1141 gattcccaca gggacaggca gaatatcgac cactttccgc gtcatctcag cgatttcgtt
     1201 gggcaggctg ccgccgttgg tgtcgcagag caccactgtg cgggcaccgg cctctgccgc
     1261 cacgcgaact gcggtcaatg cgtaatcggc gtcgtacttg taaccgtcga aaaagtgttc
     1321 ggcgtcgaaa atgacttctc
//
LOCUS       DAOPTR010001112         1339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_77662_length_1339_cov_3.659657, whole genome shotgun sequence.
ACCESSION   DAOPTR010001112 DAOPTR010000000
VERSION     DAOPTR010001112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77662_length_1339_cov_3.659657"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PKI05_16140"
     CDS             complement(<1..500)
                     /locus_tag="PKI05_16140"
                     /inference="COORDINATES: protein motif:HMM:NF039495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53783.1"
                     /translation="MAHPNAQPVIAVQGSGAMAATTLLGQGPARIPTGGKIRAGIKVL
                     TKKAAGEPKAKAIYDQGVAAGQSFEQIERAIAEALPNLKTPLVPRNVPWFTVRAQDFP
                     NPEIAREILDAYGEDRGEGRRLYRFPVVFPSDYWQTVMPHELAAWGAHEKHYWSQYSA
                     DGRVRH"
     gene            complement(802..>1339)
                     /locus_tag="PKI05_16145"
     CDS             complement(802..>1339)
                     /locus_tag="PKI05_16145"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOA53784.1"
                     /translation="SRQARSAAVVGALQAVVRQTQRLDLMPAITVLQEYADTLPEEGR
                     RSIYEDAVVFAREWDQYLRSDGSARTIGGFMSSKALGATQHASREGVALLTVHSSKGL
                     EFDVVFVAGMAEGVFPDYRAVGKKRELEEEARNAFVAVTRSKRLLYLTYPSVRMMPWG
                     ERRGQQPSRYLRAAGLVP"
BASE COUNT          252 a          437 c          381 g          269 t
ORIGIN      
        1 cagtggcgca cccggccatc ggccgagtat tgcgaccagt agtgcttttc gtgcgcaccc
       61 caggccgcca actcgtgcgg catcacggtc tgccagtagt cggaggggaa caccaccggg
      121 aagcggtaca aacgccgtcc ttcgcctcga tcctcgccat aggcgtccag aatctcccgg
      181 gcgatctcgg gattgggaaa gtcctgcgcc cgcacggtga accacggcac attgcgcggc
      241 accagcggcg tcttgaggtt gggcagggct tcggcgatgg cacgctcgat ctgctcgaac
      301 gactggcccg cggccacgcc ttggtcgtag atggccttgg ctttgggctc tcccgcggcc
      361 ttcttggtga gcactttgat gccggcgcgg atcttgccgc cggtgggaat gcgggcgggg
      421 ccttgcccta gcagggtcgt tgcggccata gcgccgctgc cctgcacggc gataactggt
      481 tgggcgttgg gatgtgccat gcgggcgctc ctccatgaac gtcgggacat tccgacaatt
      541 catggtctcg cgacatcctc gctcttcaac cccgcgccac ccccagccgt ttacgcccct
      601 catgcccaat tgatagaatg gagcacgctg ccagtcttgc ttgcatacga gcgaaacgca
      661 cggtctgaag cgttccaccg cccaagtcgc cagtgtttgt caacggcgat ttaaaactga
      721 cactcttttt cttcgaacag cgatctaaaa ccgggacacc gcaacgatct gtggcaaatg
      781 tttagataaa gctgacacta cttaaggcac aagtcctgcg gctcgcaaat accgcgacgg
      841 ttgctggcct ctccgctcac cccacggcat catcctcacg ctgggatagg ttaagtagag
      901 cagcctcttc gacctcgtga cggcaacgaa tgcgtttcgg gcttcctcct ccaattcacg
      961 tttcttcccc acagccctgt aatcgggaaa gacaccttcg gccattccag cgacaaagac
     1021 gacgtcgaac tcgaggccct tggaggagtg aactgtcagg agggcaacac cctccctgga
     1081 ggcgtgctgc gtcgcgccta atgccttgct actcatgaac ccgccaatgg ttctcgcgga
     1141 cccgtccgag cgtaggtact gatcccactc ccgggcgaac accacggcgt cctcgtaaat
     1201 tgaccgccgt ccctcctcag gcagggtgtc ggcatactct tggagaactg tgatcgccgg
     1261 catcaaatca agacgctggg tttgtcgcac cacggcctgc aatgcaccga cgaccgctgc
     1321 ggatcgggcc tgccgtgat
//
LOCUS       DAOPTR010001113         1339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_77686_length_1339_cov_2.623053, whole genome shotgun sequence.
ACCESSION   DAOPTR010001113 DAOPTR010000000
VERSION     DAOPTR010001113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77686_length_1339_cov_2.623053"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="PKI05_16150"
     CDS             complement(<1..846)
                     /locus_tag="PKI05_16150"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOA53785.1"
                     /translation="MRKALYWFCVVVVVFSLSAGVAKADSLDSGWESPPREARLRAYW
                     WWLNGCVTPQAITRDLEEMKAKGFGGALICDADGSSQDGNERAPHGPDFFSPQWRKLF
                     KYTLQEADRLGLEMSLNIQSGWNLGGPVVSADDAGKKLVWSEHRVTGPCEFRSRLPQP
                     AHRDGYYRDAIVVAYPVKTVSVESPEVRVTASSGQPAHPVDDLADGNPETFWASGGGQ
                     PGQGPSPDRPEWVDFTFTSPVKINRVILRPRPMYGPHKCRLLVSDDGREFRTVADLTL
                     PNPRDE"
BASE COUNT          319 a          406 c          355 g          259 t
ORIGIN      
        1 ttcgtcccgg ggatttggca gcgtcaggtc ggcgacggta cgaaactcac gaccgtcatc
       61 cgagacgagc aaacgacact tatgcggccc atacatcggg cgagggcgca agatcacacg
      121 gttgattttg acaggcgatg tgaaagtaaa atcaacccac tcaggccgat ccggcgaagg
      181 tccctgtccc ggctggcccc ctccggaagc ccaaaacgtt tccgggttcc cgtccgcaag
      241 atcatcgact ggatgggccg gttgtcccga cgatgcggtc acccgaacct ctggagattc
      301 gacggacacg gttttcacag gatacgccac aacaatcgca tcgcggtaat agccgtcacg
      361 gtgtgccggt tggggaaggc gcgaacgaaa ctcgcaaggt ccagtcacac gatgctctga
      421 ccatactagt tttttgcccg cgtcatcggc ggagacaaca ggtcctccaa gattccagcc
      481 actctggata ttgaggctca tttccagccc caagcgatcg gcctcctgca aggtatattt
      541 gaacaatttc ctccactgcg gtgagaagaa gtcgggaccg tggggtgcgc gttcattgcc
      601 atcctggctc gatccatccg cgtcgcaaat gagtgccccg ccaaagccct tggccttcat
      661 ttcctcaagg tcccgcgtaa tcgcctgagg ggtcacacag ccgttcagcc accaccaata
      721 agcgcggagg cgcgcctcgc ggggtggcga ctcccagcct gaatcaagtg aatccgcctt
      781 ggcgacaccg gctgacaacg aaaaaacgac cacaaccaca caaaaccaat acaacgcctt
      841 tctcatggta gcgcaatcct gcaaaagact tcgaaagatt tctcggtact gtgctgtatt
      901 atggaacacg atccgcccct ttgcaatttg acgaagacgt tcgccgagca cactgcggta
      961 ggagacacca agcacactgc gaaaggagac gtcatttcag ggtaatttgc gaacctactt
     1021 tcccaacggg tctcgaatgc agaaattgag atacctcgct gttcactcgg gattatcaag
     1081 aaggatagaa aaggtccgcc tttgctggtc agaggcgaat aaagcacgac ggccgtcccg
     1141 ctaacaccct tcgctaaaga ccccttagac aacccatgac gaaagcctcg gccggctaag
     1201 agcgtatccc gtaacctccc ctcagtcgct cggcgggaga ggcgtccggg ggtgagggtg
     1261 gctcgaatgg ttactggttt tctcgatcga ctccgacgat cacttgccct caccccaacc
     1321 ctctcccaga gggagaggg
//
LOCUS       DAOPTR010001114         1336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_77923_length_1336_cov_3.101483, whole genome shotgun sequence.
ACCESSION   DAOPTR010001114 DAOPTR010000000
VERSION     DAOPTR010001114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77923_length_1336_cov_3.101483"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            338..>1336
                     /locus_tag="PKI05_16155"
     CDS             338..>1336
                     /locus_tag="PKI05_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53786.1"
                     /translation="MKPITTISGLSYRSLVAYLPSHLAVLLAAATTTAVLTGALILGD
                     SLRFSLRQRAIDRLGEIDWVLRVPHLFRWSWADEVTEIEEFRRCFSHTAIVLHIQTVL
                     EKPGDAPRVVNRVQLFGCDARFWAMGSGQPQHIPRAREIVLNQTLANRLKAKVGDRLT
                     LRIPQLQGIPSESALGRRHDLVRPFAVTVADIVPDVSLGAFSLNLSQEIPCNAYVNLG
                     WLASATGQPGKANLLLLKTANNSSHSSEEAERVLGNLLLPPEDYGVRIERTAKGYVNI
                     TTDRLIFQPSIEEHIVKRLQGVSFERVTVFLAETVRAGAKEAWYVTMAAMPLAKEPP"
BASE COUNT          279 a          365 c          366 g          326 t
ORIGIN      
        1 ggggcgtgtt gtcacgcgac cacgcccctt gctaacaccc gcagccacaa tttgcagccg
       61 acttcctttg ttgtggagtc aatggcgcga aggtcatgtc caatcttggc aaatctcagc
      121 cattggccaa agagtgtcaa acacgagtgt cgccgcgact tcgtggtgac accgtcggag
      181 tacatcaatc gcaccatcgt aacagcaaag gcccgtgtga aatgccgttt tttccattcg
      241 ctgttagttc ttctcctgat gatcaacccg cggtcggatg gccagaggca aagccttata
      301 atgattaacg tttcggagta tggagtgcct tcggtccatg aaacccataa cgaccatctc
      361 aggtctgtcc tatcgctcgt tggtggcgta tcttcctagc catctggcgg tgttacttgc
      421 tgcggccaca accacggcgg ttttgaccgg ggcactgatc ctcggtgatt ccctccgctt
      481 tagtctgcgt caacgcgcga tcgatcgcct cggtgaaatt gattgggtct tgcgtgtccc
      541 tcatttattc cggtggtcgt gggcggacga ggtcaccgag attgaggaat tccgtcgctg
      601 tttttctcat acggctatcg tcttgcatat tcagaccgtg ttggaaaaac ccggcgacgc
      661 gccgcgtgtg gtgaatcggg tgcaactttt cggatgcgat gctcgatttt gggcgatggg
      721 gtctggacaa cctcaacaca ttcccagggc gcgagaaatc gtccttaatc aaacattggc
      781 caatcgcctg aaggccaagg tcggcgatcg tttgacattg aggattcctc aactccaagg
      841 aatcccctcg gaaagtgccc tgggcaggcg gcacgacctt gtgcgccctt ttgcggtcac
      901 cgtggctgat atcgtgcccg atgtaagtct tggtgccttc agcctgaact tgtctcaaga
      961 gattccttgc aatgcctacg tcaacttggg gtggttggcc tccgccacag gccaacctgg
     1021 caaggccaac ctccttcttc tgaagaccgc taacaattca agccattctt cggaagaggc
     1081 agaacgtgtg ttagggaacc ttttactacc accggaagac tacggggtgc ggatcgaacg
     1141 cacggccaag gggtacgtaa atatcaccac cgaccgactt attttccagc cgagcattga
     1201 agagcacatt gttaaacgcc tgcaaggtgt ctcatttgag cgggtaacgg tttttttggc
     1261 agaaacggtc cgcgccgggg caaaggaggc ttggtacgtc acgatggctg ccatgccttt
     1321 ggcgaaggag ccgcca
//
LOCUS       DAOPTR010001115         1333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_78169_length_1333_cov_2.762128, whole genome shotgun sequence.
ACCESSION   DAOPTR010001115 DAOPTR010000000
VERSION     DAOPTR010001115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78169_length_1333_cov_2.762128"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..210)
                     /locus_tag="PKI05_16160"
     CDS             complement(31..210)
                     /locus_tag="PKI05_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53787.1"
                     /translation="MNTTEGNEQSPVATIPPPEQIRARLRVLQAEADILRKLLRASER
                     LQKARATTPEVPYAK"
     gene            complement(335..>1333)
                     /locus_tag="PKI05_16165"
     CDS             complement(335..>1333)
                     /locus_tag="PKI05_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53788.1"
                     /translation="RRRRLNHLPARPAEPARLKSRWNMSEKKHVLNFIEQAVIDSLYR
                     FQEGLYPKDGELHDEHGNVIESGFLLRESIRPVPKMLLCASLWPKTSADKKTSVGLQG
                     MEAALSVLQEHGLVREMPRPRMVYGAWKRVDGLIFESLETARAGEAKYRIRRANGEPI
                     LEADPFTIEGATWTRGYELTSAGIDHARNTQAAPTTAHATKNGEVTITDAMSLKDTPP
                     RPATAAPDGPQPPNLLQFQGKLYKLQPLHWQIVNYMWNRGSAPIEDAIEAIWGADASD
                     NTFRAAVSKVNGRLSEIGAITAIELSVKNGYLVNSALADSESGRALFAVQPSPSPR"
BASE COUNT          243 a          372 c          395 g          323 t
ORIGIN      
        1 cctttggcgt ccagggcgat aggtaggggc tcatttggca tagggcacct ccggcgtcgt
       61 cgctctagcc ttctgcaacc gctcagaagc gcggagtagc ttccgcagaa tgtccgcctc
      121 ggcctgcaac acgcgaagcc gggcgcggat ctgttcgggc gggggtatgg tcgcaacggg
      181 cgattgctcg ttgccttcgg tagtgttcat tggttggcct ccattattca tcatggtggc
      241 ctattagatc gcggggggta ttgccaaact gttaccattg acggcaacaa atcggcaaca
      301 aatcggcaac aaaatcggca acacaaggtc aagattagcg gggggagggc gaaggctgga
      361 cggcgaaaag tgcccggccg ctttcagaat cggccagggc ggagtttacc agataaccgt
      421 ttttaacact gagctcgatt gctgtgattg cgccgatctc gctcaagcgg ccgttgacct
      481 tcgatactgc cgccctgaac gtattatcgc tggcgtctgc accccaaatg gcctcaatcg
      541 cgtcttcaat gggagcagat ccgcgattcc acatgtaatt gactatctgc cagtggagcg
      601 gctgtagctt gtaaagtttt ccctggaatt gcagcaaatt tgggggctgt gggccatcgg
      661 gggccgctgt tgccggtcgt ggcggtgtgt ccttaaggct cattgcatca gtaattgtaa
      721 cctctccgtt cttcgtcgca tgtgccgtgg tgggcgcagc ctgcgtgttt ctcgcatggt
      781 cgatgccggc cgaagtcagc tcatagcccc tggtccacgt ggctccctcg attgtaaacg
      841 gatcggcctc caggatgggt tctccgtttg cccggcgaat acgatacttg gcctctccag
      901 cgcgggcggt ttcgagagat tcgaagatta gaccgtctac ccgcttccat gccccataga
      961 ccattcgggg gcgcggcatc tctcttacca acccgtgctc ctgtagtacg ctcagtgcag
     1021 cttccatgcc ctgtagcccc acggaggtct tcttgtcggc agaggtcttg ggccacaagc
     1081 tggcacaaag cagcattttt ggaacagggc ggatcgattc cctcagaagg aatccgcttt
     1141 cgatcacgtt tccgtgttcg tcgtgcagtt cgccgtcctt ggggtagagt ccttcctgga
     1201 aacggtaaag gctgtcaatg accgcttgct cgatgaagtt cagaacgtgt tttttctctg
     1261 acatgttcca cctacttttc aaccgggccg gctcggcggg acgggctggt aggtggttca
     1321 gccgtcgccg ccg
//
LOCUS       DAOPTR010001116         1333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_78175_length_1333_cov_2.495305, whole genome shotgun sequence.
ACCESSION   DAOPTR010001116 DAOPTR010000000
VERSION     DAOPTR010001116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78175_length_1333_cov_2.495305"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1333)
                     /gene="carB"
                     /locus_tag="PKI05_16170"
     CDS             complement(<1..>1333)
                     /gene="carB"
                     /locus_tag="PKI05_16170"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOA53789.1"
                     /translation="TIMVNSNPETVSTDYDTSDILFFEPLTCEDILNIQDRVQADGVI
                     VQFGGQTPLNLARALQVAGVPIIGTSVDTIEDAEDREKFAEVLRRLNLRQPPNATART
                     FSEAQLAIQRIGFPVLVRPSFVLVGRAMEICYDMNQFEEFVKKAFDAAEGQPVLIDRF
                     LEDAIEVDVDAIADGDLAIVAGIMEHIEEAGIHSGDSACAIPPYSLPGPVVEEIRQAT
                     QLMAKALNVKGLMNVQYAVKKEDGRPVVYVLEVNPRASRTVPFVSKAIGWPLAKSASL
                     VMVGIPLREQGWTKAPTPTRVSVKEAVLPFVKFYGVDIVLGPEMKSTGEVMGVSRYFS
                     IAYAKSQIAAGTALPRSGRIFISVADRNKHQIVELARRLQNLGFELLATKGTADRLEQ
                     NGITVTRLKKIKEGHPNVLDYILNGDIQLIINTPSGKGARTDEGHIRASAIA"
BASE COUNT          300 a          382 c          338 g          313 t
ORIGIN      
        1 cgcaatggct gacgcccgaa tatggccttc gtctgttcgc gcccctttgc cgctgggggt
       61 gttgataatc aactggatat ctccattcag gatgtaatcg aggacgttgg gatggccttc
      121 tttgattttc ttgagacggg tcacagtgat accgttctgt tccaaccgat cggcggttcc
      181 ttttgtggcg agtaattcga agcccaagtt ctgcaggcgg cgagccagct ccacaatttg
      241 atgcttattt cgatctgcaa cactaatgaa gattcgacca gacctgggca aagccgtccc
      301 ggcggcgatt tggctcttgg cgtaggcaat cgagaagtag cgactgactc ccatcacctc
      361 gccggtggac ttcatttccg gtcccagcac aatatcaaca ccgtaaaact tgacgaaagg
      421 aagtaccgcc tctttgaccg agacgcgcgt cggcgttggg gctttggtcc agccttgttc
      481 ccgcagtgga atcccaacca tcaccagcga tgcagacttc gccaaaggcc agccaatggc
      541 cttcgacaca aatggtacgg tacgacttgc ccgtggatta acttccagaa catacaccac
      601 cggtcggccg tcttcctttt tgacggcata ctgcacgttc attagcccct tcacattcaa
      661 ggccttggcc atgagctgcg ttgcctggcg gatctcttcg accacaggtc ccggcaagct
      721 atacggtgga atagcgcagg ccgagtcacc tgaatggatg cctgcctctt cgatgtgctc
      781 cataatcccg gccacaatcg ccaaatcgcc atccgcgatc gcatcgacat cgacctcaat
      841 ggcatcttcc agaaagcgat cgatgagcac aggttggcct tcagcggcat caaaggcctt
      901 tttcacgaat tcttcaaatt ggttcatgtc gtagcaaatt tccatagcgc ggccaacgag
      961 cacaaaactc ggtcgtacga ggaccgggaa accaattcgt tgaatggcca attgggcctc
     1021 ggagaacgtc cgcgcggtag catttggagg ttgacgcaag ttcaatcgcc gcaggacctc
     1081 tgcgaatttt tctcgatctt cggcatcctc aatggtgtcc acactggtgc cgatgatcgg
     1141 gacccccgcc acctgcaggg ccctggccaa attaagaggt gtctgacctc caaactgaac
     1201 aattacgcca tcagcctgga cccgatcctg gatgttgagg atatcctcac acgtcaaagg
     1261 ttcaaaaaag aggatgtcac tcgtgtcata gtcggtactg accgtctccg ggttggaatt
     1321 gaccatgatc gtt
//
LOCUS       DAOPTR010001117         1332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_78243_length_1332_cov_3.198904, whole genome shotgun sequence.
ACCESSION   DAOPTR010001117 DAOPTR010000000
VERSION     DAOPTR010001117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78243_length_1332_cov_3.198904"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1332)
                     /locus_tag="PKI05_16175"
     CDS             complement(<1..>1332)
                     /locus_tag="PKI05_16175"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HOA53790.1"
                     /translation="GKDLSWLKNYETYSSIFAVKCTHDFFGAYDVDADRGVVQWADHR
                     QLSGKKAWTWGEWDFGRMAEQDLTDDDGPYIEVQSGPLPTQSDYGRLAPHQRVAWQEW
                     WYPAHGLGDGFEYATRDAVVNVLRRRDGVQVRILGTGVFPRANCVIKAGKTVLRQATV
                     DLSPLQPAVLEIPVNPSEAITIEVRSANGEVLVNHRSPLEVLPVEPPDPATFRETPDD
                     KKTAEEWYRKGEKYDLATDRLAARRHYRMALERDPGYTPARRALAILDFEAGLYPAAV
                     DALKDVVARDPNDGWAWFYLAASHFRLKNLPDALNAAQKAVALKTPGVPAADLLGRIL
                     MVQNNHVEAEKAFRQALEQSPDDSVAMDHLILCLVAQKREREALELIRRRTEGNTTAI
                     LPVWALAQIGGDKEKSVLIARANALGDPQFEVLEVALFLDEVGMSAEAEDL"
BASE COUNT          268 a          413 c          387 g          264 t
ORIGIN      
        1 atcaaatcct ccgcctccgc ggacatgccg acctcgtcaa ggaacagggc cacttccagg
       61 acttcgaatt gcgggtcacc gagtgcgttc gcccgagcga tgaggaccga tttctcctta
      121 tcacctccta tttgggccaa ggcccaaacc ggcaaaatgg ccgtcgtatt gccctccgtg
      181 cggcggcgga tcagctcaag ggcttcacgc tcccgcttct gtgcgaccag gcacaggatg
      241 agatggtcca tggccaccga gtcgtccggg gattgctcga gggcttgacg aaaggccttt
      301 tccgcctcga cgtggttgtt ttgaaccatc agaatgcgtc ccaaaaggtc tgctgccgga
      361 acgcccggtg ttttgagagc aacagccttc tgcgcggcgt ttagggcatc gggaaggttc
      421 ttcaaacgaa aatggctggc ggccagatag aaccaagccc agccgtcatt gggatcgcgc
      481 gccacaacgt ccttaagggc atcgaccgcc gccggataaa ggcccgcttc aaagtccaaa
      541 atggccaggg cacgccgggc cggggtgtaa ccagggtctc gttccagggc catgcgataa
      601 tgtcgccggg cggccaaacg atcggtggcc aggtcgtact tttcaccctt acggtaccac
      661 tcctcggccg tctttttgtc gtccggcgtc tcgcggaagg tggccggatc aggcggttcg
      721 acgggcaaga cttcaagcgg cgaacgatgg ttgacaagga cctcaccatt cgccgagcgc
      781 acctcgatgg tgatcgcctc tgaggggtta actggaatct caagaacggc cggttgaagc
      841 gggctgaggt caacagtggc ctgcctcaag acggttttcc ctgccttgat aacgcagttt
      901 gcccgcggga agacacctgt ccccaagatg cggacttgaa ccccatcgcg tcgccggaga
      961 acgttcacga cagcgtcacg agtggcatat tcaaatccgt cgcccaagcc gtgcgccggg
     1021 taccaccact cctgccaggc cacccgttga tgtggagcga gacgcccgta atctgactgc
     1081 gtgggcagcg gcccgctttg aacctcgatg tagggaccgt cgtcatcggt caggtcttgt
     1141 tcggccatcc ggccaaagtc ccactctccc catgtccagg ccttcttacc gcttaactgg
     1201 cgatggtccg cccattggac gacaccccga tcggcatcga catcataggc cccaaagaaa
     1261 tcgtgcgtgc acttgacggc aaagatcgag gagtaggttt cgtagttctt cagccaagag
     1321 aggtccttcc cc
//
LOCUS       DAOPTR010001118         1327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_78693_length_1327_cov_3.152516, whole genome shotgun sequence.
ACCESSION   DAOPTR010001118 DAOPTR010000000
VERSION     DAOPTR010001118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78693_length_1327_cov_3.152516"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..>1327
                     /locus_tag="PKI05_16180"
     CDS             30..>1327
                     /locus_tag="PKI05_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53791.1"
                     /translation="MSRWRFRLGFHPDLGTVRIVRSRRRGFILLLVILTIISAMALTY
                     VVASTTRLSMRKVRNAQLQKAAIAATETGLSVGLANMCSSKWGGIGSQISGACGLNQA
                     FEVTYEHGDPTLDAASPEWTDCPYRVTIRAIGKAWHPEEPEYPAIYRRSWVVQLVPRA
                     VAAEPSDWSRILNYTFFQTAVTASSVEIPVRIVGKCRFQSTLNLAVKYPTYNPRVRYM
                     SDLYEMQANGYGDWRTFTGRIYFPVSSQPLDTIDLLVNRLRVFINDAWVNLPSSDLAL
                     PQQPTSYRLYPGGKSYSIPQLGSALENTTLSPDPDNNPCGILYAPGNIAIRSNVTIKG
                     SLLCNGDVRIEGTNIVISGQPIWGIQADVPVELPVIVCKNLHFKAGSACQINGMVGLF
                     GELQAPKAPTLTIVEITGRVFATAIKIDSLSPWDSFDWDKA"
BASE COUNT          288 a          342 c          367 g          330 t
ORIGIN      
        1 attacctgtg ttgccatctt tcacggacca tgagtcgctg gcgattccga cttgggtttc
       61 atcctgactt gggcacggtg cgtatcgtcc gttcgcggcg tcgggggttc attctcctac
      121 tcgtgattct gacgatcatt tcggcaatgg ctttgacgta cgtcgttgct tcaacgacgc
      181 ggctgagcat gcgaaaggtt cgcaacgccc agttgcagaa agctgccatc gctgccacag
      241 agacgggctt gtcggttgga ttggccaaca tgtgttcttc caagtggggg ggcattggca
      301 gccaaataag cggggcctgc ggcttaaatc aagcttttga ggtaacctac gaacatggtg
      361 acccgacgct cgatgccgcg tcaccggaat ggactgattg tccgtatcgg gtgaccattc
      421 gcgcaatcgg caaggcatgg cacccagaag aacctgaata ccccgccatt tacagaagga
      481 gctgggttgt tcaacttgtg cctcgggctg tagcggcgga gccttccgat tggtcgagga
      541 tccttaatta cacctttttc cagacagcgg tcacggcttc atctgtggag attcctgtgc
      601 gaattgtggg caagtgtcgg tttcaaagta ctctgaacct ggcagtcaag tatccgacgt
      661 acaatcctcg ggtgaggtac atgagcgatc tttacgagat gcaggccaat ggatatggag
      721 actggcgaac gtttaccggc cgtatctatt tccctgtctc gtcccaacct cttgacacca
      781 ttgaccttct tgtcaatcgg cttcgagttt ttataaatga cgcgtgggtc aacctgccca
      841 gtagcgacct tgccttaccg cagcagccca catcgtaccg gttgtatcct gggggcaaaa
      901 gctattcgat tccgcaactg gggtcggcat tggaaaacac gacgctatcg cctgacccgg
      961 acaataatcc gtgtggtatt ctgtatgcgc caggaaacat cgcgatacgt tccaacgtca
     1021 cgatcaaagg gagtctgctc tgtaatgggg acgtccggat cgagggaaca aacattgtga
     1081 tcagtgggca accgatctgg ggaatccaag ccgacgtccc cgtggagtta cctgttatcg
     1141 tatgcaagaa tctccatttt aaggccgggt cggcatgtca gatcaacgga atggttggcc
     1201 tttttggtga gttgcaggcc cccaaggcgc caaccttgac gatcgttgag atcacaggcc
     1261 gagtctttgc tacagcgatc aaaatcgatt cattgtcgcc atgggactct tttgattggg
     1321 ataaagc
//
LOCUS       DAOPTR010001119         1315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_79686_length_1315_cov_3.151587, whole genome shotgun sequence.
ACCESSION   DAOPTR010001119 DAOPTR010000000
VERSION     DAOPTR010001119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79686_length_1315_cov_3.151587"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PKI05_16185"
     CDS             <1..481
                     /locus_tag="PKI05_16185"
                     /inference="COORDINATES: protein motif:HMM:NF039585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HOA53792.1"
                     /translation="PAVKQWLSQAEGSSLLQQLETIGQVIVPLPDSEISLTKEDIQIR
                     LNAKPGWTAAQGRVGVVILSTQLSPALIAEGLARDFVHAVQNRRKELDCDFVERIEVG
                     VVTQSESIKQAIRDYQDYIMAETLATSLQFSPLTGTTPTTLNIGQQQVELYVKPVRN"
     gene            874..>1315
                     /locus_tag="PKI05_16190"
     CDS             874..>1315
                     /locus_tag="PKI05_16190"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOA53793.1"
                     /translation="MPSLFVIRGNDQGTRFELEGSTITIGRDSSNQVQLHDTGVSRHH
                     AEIKRVENRFLLVDLNSSNGTLVNGKRIQEHWLTSGDQIQMGRTLMLFTSPAEDAQPE
                     TPWNVAISSEPDEDRSQIVSALKQEEGSRFFANAVVGNDPWTVQA"
BASE COUNT          321 a          388 c          351 g          255 t
ORIGIN      
        1 ccctgccgta aagcaatggc tttctcaggc agaaggaagc agcctccttc agcaactgga
       61 gacgattggt caagtcatcg tgcccttgcc cgatagtgag atttccttga ccaaagagga
      121 tattcaaatc cgcctcaatg ccaaaccggg ttggacagcg gcccaaggcc gggtgggcgt
      181 ggttattctc tctacccaat tgtcgccagc actcatcgcc gagggccttg cccgcgattt
      241 cgtccacgcg gttcaaaata ggcggaagga actcgattgc gatttcgtcg aacgaatcga
      301 agtcggtgtc gttacgcaat cggagtcgat caagcaggcc atccgtgact accaagacta
      361 catcatggcc gaaaccttgg ccacatcgct tcagttttct ccgctcaccg ggacaacgcc
      421 cacaacgttg aacatcggtc aacagcaagt cgaactctat gtcaagcccg tgagaaacta
      481 acccgagcgt atcggccttc gaccgacgtg atgtttccaa gactagcgtc accaaggcat
      541 tagtttagag tctttcccaa aaagtctccc ctctccctcg gggagagggt tggggtgagg
      601 gcaagcgaac gtcggaatcg atcgagaaaa ccggcagcga ttcgagccac cttcaccccc
      661 gactcttcta gcgccgaacg ggcgagggga gagtatggga catgctctta atgacctccg
      721 gcgatcgcgg tagtcctggc atgacaacaa aacaggggtg tcacaccagc cctcgaatcc
      781 ttagaataca agaggatact ggcggctgtg gcgttaaccc actgattgtt acggaaaaca
      841 agtgcccaat tccacaaggg gggacaccga gtcgtgccat ccttgtttgt gatccgcgga
      901 aatgaccaag ggacccgttt cgagctggag ggctcaacca tcaccattgg gcgagactcc
      961 agtaatcagg tccagctcca cgatacgggt gtctcccgcc accatgccga gatcaaacgg
     1021 gtggagaatc gcttcctttt agtggacctc aatagctcta acgggacgct cgtcaacgga
     1081 aagcggatcc aagaacattg gctgacgagt ggcgaccaaa tccaaatggg gcgaaccctc
     1141 atgctcttca cctccccggc ggaagacgcc cagccggaaa ccccgtggaa cgtcgccatt
     1201 tcctctgaac cggacgaaga ccgctcccag atcgtcagcg cccttaagca ggaggaaggt
     1261 agccgctttt tcgccaacgc cgttgtgggc aacgacccct ggacggtcca agcca
//
LOCUS       DAOPTR010001120         1315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_79725_length_1315_cov_1.935714, whole genome shotgun sequence.
ACCESSION   DAOPTR010001120 DAOPTR010000000
VERSION     DAOPTR010001120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79725_length_1315_cov_1.935714"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="PKI05_16195"
     CDS             <1..1046
                     /locus_tag="PKI05_16195"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOA53794.1"
                     /translation="VQIAETQVKVTPSPSPDPALTPKPAPAIPVHSDLANIWKHKGII
                     FVVAGACSILLLIGLLALSWQEPSSELSDSRSHDGTLYGNPEGWQSGLDGDSWKPAQE
                     TTFSEEDEYDTGTMAGGRPNRTASNRVQLSASESETESPGWLEQLQKATVTIITDTGS
                     LGSGLFIHDQVCGFVLVTNNHVVEGASVIRAQLHDGTRVGVRRGALYPQFDLAFLAVD
                     GLEQPPAVLTLRNELPKLAEKVFAYGAPMGLKGTITEGIVSAVRTTKELQEPGGGPFD
                     EFDGEMCWIQTSAPVSPGNSGGPLVDSHGRVIGVNTFASFYVQNLNFAISAQQIIALL
                     PELQMASLPPPQR"
     gene            1110..>1315
                     /locus_tag="PKI05_16200"
     CDS             1110..>1315
                     /locus_tag="PKI05_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53795.1"
                     /translation="MKTALQVWKSLNSDLGRLEQVSPAKQARILRNLAASCAGMIAYL
                     NDLDDSNVDREAIKCKDAICDLMNT"
BASE COUNT          312 a          337 c          359 g          307 t
ORIGIN      
        1 cggtgcagat tgccgaaacc caagtcaagg taactccatc tccttcgcct gatcccgcat
       61 tgacgcccaa accggcacca gcgatccctg ttcactctga tttggctaat atttggaaac
      121 acaaagggat tatttttgta gttgcgggcg cttgttccat ccttttattg atcggtcttt
      181 tggccctcag ttggcaagaa ccctcgtcgg aattgtcaga cagccgatcg cacgacggaa
      241 cgctgtacgg caatcccgaa ggctggcaaa gtgggcttga tggggattcc tggaaacctg
      301 cgcaagaaac taccttttca gaagaagatg aatacgacac cgggactatg gcaggaggcc
      361 gaccaaaccg gacagcctct aatcgcgtac agctttctgc atcggagtcc gagaccgagt
      421 cgcccggttg gctggaacaa ctccaaaagg caaccgttac cattatcact gacaccggaa
      481 gcctgggtag cgggcttttc atccatgatc aagtgtgtgg cttcgtgctt gtcacgaaca
      541 accatgtggt ggaaggggcc agcgtgatcc gagcgcagtt gcacgacggt acccgggtgg
      601 gtgtaaggcg tggggcgctc tatcctcaat tcgatttggc ctttttggcc gtggatggat
      661 tagagcaacc cccggctgtg ctcacactgc gtaacgaact tccaaagcta gctgaaaagg
      721 tctttgccta tggcgcgcct atggggctaa agggaacaat caccgagggg attgtcagtg
      781 ccgtgcggac gaccaaggaa ttacaggaac caggaggagg tccgtttgat gaatttgatg
      841 gagagatgtg ttggattcaa acctcggctc ccgtctcacc tggcaatagc ggtggtccat
      901 tggtcgattc gcacggacgg gtaatcggcg tgaatacgtt tgcgtctttt tacgttcaga
      961 atctcaactt cgccattagt gcccaacaga tcattgcact cttgccagag ctacaaatgg
     1021 caagtcttcc cccaccgcaa cgataggcac ctgaagcagg cagtaacaat gcgatctcag
     1081 cttgggcgac ctttaattac tggttggcca tgaagaccgc cttgcaagtc tggaaaagtt
     1141 tgaactccga cttaggtcgg ctcgagcaag tttcgccagc gaaacaggca cgaatcctgc
     1201 gtaatcttgc cgcgtcttgc gcggggatga ttgcctatct taacgatttg gacgactcga
     1261 atgtcgatcg tgaggccatt aagtgcaagg acgcgatttg cgatttaatg aacac
//
LOCUS       DAOPTR010001121         1313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_79855_length_1313_cov_3.855326, whole genome shotgun sequence.
ACCESSION   DAOPTR010001121 DAOPTR010000000
VERSION     DAOPTR010001121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79855_length_1313_cov_3.855326"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="PKI05_16205"
     CDS             <1..777
                     /locus_tag="PKI05_16205"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOA53796.1"
                     /translation="FLLTNILTRIGVSWNLVQQALISQVVVILLPVVVLAALLTREIR
                     ATFLLRLPSWRAVIGAVLLAVMLHPAFFTWQQVIVRLYPLDPVLKESLKQLTGQALDL
                     RQLLLVMALLPAICEELAFRGFILTGFRSSLRPTHALIFAALFFALGHTILQQSMVAF
                     AAGLILGVIAIQTGSLLPCVIYHAIHNGLLILQGSLGQSRWVSDPWIKAMMTWTDEGP
                     TYHLSVVVFGILAAGLIFAWMISRPEVLRSYLAEEETGSH"
     gene            794..>1313
                     /locus_tag="PKI05_16210"
     CDS             794..>1313
                     /locus_tag="PKI05_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53797.1"
                     /translation="MTSAADLCVTADWILPLDGPPIPNGRITIAGHRIAEIDAGGERV
                     RLMRHDPRPQGVTHLHYKDCVILPGLINAHTHLDLSGWSGDFISPPKLWDWIPEVVRF
                     RRSNAYRPGEAIRDGLQAVLAGGAVGVVDVIPPDWFTWGLVPEQGADPDGDQQMPEWE
                     PSRLRVLSMIECL"
BASE COUNT          231 a          365 c          368 g          349 t
ORIGIN      
        1 tttcttttga cgaacatcct cacccgcatt ggcgtctcgt ggaatctcgt ccagcaggcc
       61 ttgatttcgc aagtggtagt aatcctcttg ccggttgtcg ttctagcagc cttattgacg
      121 cgggaaatcc gcgcgacgtt cttgctccga ctcccgtcct ggcgtgcggt gatcggagcg
      181 gtcctccttg ccgtaatgct tcatccggcc ttttttactt ggcaacaagt gatcgttcgt
      241 ttatatccgc ttgatcctgt attgaaagag tctctcaagc aattaaccgg gcaggccctt
      301 gatttgcggc agctcctttt ggtcatggcc cttttgcccg ccatttgcga ggagttggcc
      361 tttcgtgggt ttattctgac gggatttcgg tcctcgcttc gaccgacgca tgccttgatc
      421 ttcgcagcgc tctttttcgc cctgggacac acgatcttac aacagtccat ggtggccttt
      481 gcggcagggc ttatcctcgg tgtgattgcc attcaaacgg ggagcctgct tccttgtgtc
      541 atctatcatg caattcacaa tggcttgctc atcctccagg gaagtctggg ccaaagccga
      601 tgggtcagtg atccctggat caaagccatg atgacctgga cagatgaagg acctacctat
      661 cacttgtcgg tagtggtgtt tggcatcttg gccgccggac tgatctttgc ctggatgatc
      721 tcgcgcccgg aggtgcttcg ctcctatctg gccgaagaag agaccggctc ccattgagtg
      781 agacaatccc gccatgacga gtgccgccga tctttgtgtg actgccgatt ggattcttcc
      841 ccttgacgga ccgccgatcc ccaatggaag gatcaccatt gctggtcacc gtatcgcgga
      901 gatcgatgcc ggcggcgaac gcgttcggtt gatgcgccat gacccgcgtc cacagggcgt
      961 aacgcatctt cattacaagg attgcgtcat ccttcccggc ctgattaacg cgcatacaca
     1021 tcttgatctc agcggatggt cgggcgattt catatcgcca cccaaacttt gggattggat
     1081 tcccgaggtc gtccgttttc gtaggtcaaa tgcatatcgg cctggggaag cgatccggga
     1141 tggccttcag gccgtgctgg caggcggagc cgtcggtgtg gttgacgtga tcccgcccga
     1201 ttggtttacc tggggtttgg ttcctgaaca gggcgcggac cctgacggtg atcagcaaat
     1261 gcccgagtgg gaacccagca ggctacgggt cctcagtatg attgagtgtt tgg
//
LOCUS       DAOPTR010001122         1312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_79931_length_1312_cov_4.207637, whole genome shotgun sequence.
ACCESSION   DAOPTR010001122 DAOPTR010000000
VERSION     DAOPTR010001122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79931_length_1312_cov_4.207637"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PKI05_16215"
     CDS             complement(<1..279)
                     /locus_tag="PKI05_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008682044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53798.1"
                     /translation="MNTVIAVVEKPKLNNIPEETHASPRGDSPSENVSRFVYASGSRP
                     LSGYTIKRGIGRGGFGEVYYAVSDSGKDVALKLLCRNWDVELRGINHCL"
     gene            complement(397..1113)
                     /locus_tag="PKI05_16220"
     CDS             complement(397..1113)
                     /locus_tag="PKI05_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOA53799.1"
                     /translation="MRRSRRSIDFGGAIMQPNACPGMTSDDACEVAMAGPPREGHSMR
                     RFLLWIDGVGGYLVCLGETIRIGRAGAGAVDVPLVADVAQHHAEIRRDGEGYFLESYA
                     PTWLDGNPVRQAVMHDRCRLQLGKHVILRFVRSTPLSLSARLDFESGHRTHPTTDGVL
                     LMAHNLILGPGPAAHVCCRDWRHNVILFRERDEVWCRAGRPFRVDGVPQERAARLTWS
                     SLVEGEDFALRLEEIPPSEV"
     gene            complement(1088..>1312)
                     /locus_tag="PKI05_16225"
     CDS             complement(1088..>1312)
                     /locus_tag="PKI05_16225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53800.1"
                     /translation="DESALGRVKDLIADLKAKLDVAERLVQSQGFYQGEIPVEDPNQK
                     DIVDEVTQYFEASSADREDDSKDAAITQVD"
BASE COUNT          286 a          421 c          326 g          279 t
ORIGIN      
        1 caaacagtgg ttgattccgc ggagttccac atcccaattt cgacaaagga gcttgagcgc
       61 cacgtccttc ccggagtcgc tgacggcata atagacttcc ccgaaccctc ctcgcccgat
      121 cccccgcttg atcgtgtagc cggaaagggg ccgcgaaccg ctggcgtaaa cgaaacggct
      181 cacgttctcc gagggtgagt ccccccgtgg tgaagcatgc gtctcttcag ggatgttgtt
      241 gagtttaggt ttctcaacca cggctatcac cgtattcact agaggactct cctgacttac
      301 tggcaccgac cttggatcag tgcgacggtc atcattcatt ctaacggaca cacccgttat
      361 gtgcgtccgt gtgagtgaat cacccggtct tccgcatcat acttctgaag gaggaatctc
      421 ctccaaacgg agggcgaagt cttctccctc aaccaatgat gaccatgtca atcgtgcggc
      481 cctctcttga ggaacaccat ccacacgaaa cggtcttccg gcccgacacc acacctcatc
      541 ccgttctcga aagagaatga cattgtgccg ccaatctcgg caacaaacat gggccgcggg
      601 gccaggcccc aggatcagat tgtgggccat gagcaatacg ccgtccgtag taggatgcgt
      661 cctatgacca ctttcgaaat ccaaccgggc cgaaagactc aacggtgtgc tgcgaacaaa
      721 gcgtaggatg acatgttttc ccagttgtaa gcgacaacga tcgtgcatca cggcctgccg
      781 tacggggttg ccatccaacc acgtgggcgc atagctctcc agaaaatagc cctctccgtc
      841 gcgacgaatc tccgcatgat gctgcgctac gtcggcgacc agggggacat ccacggcccc
      901 cgcaccagcc cggcctatcc ggatggtctc tcccagacag accaggtacc ctcccacgcc
      961 gtcgatccag agaagaaacc gtcgcatgga atgaccttcc cgcggtggac cagccattgc
     1021 gacctcacag gcatcatccg atgtcatccc ggggcaggca ttcggctgca tgatcgcacc
     1081 cccgaaatca atcgacctgc gtgatcgccg catccttgga gtcgtcttcc ctgtccgcgg
     1141 agctggcctc gaagtattgc gttacctcat ccacaatgtc tttttggttg ggatcctcaa
     1201 caggaatctc tccttggtag aatccctgcg actggactaa ccgctccgcg acatccaatt
     1261 ttgccttaag gtcggcaatc aaatctttga ctcgtccgag cgcgctttca tc
//
LOCUS       DAOPTR010001123         1310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_80110_length_1310_cov_3.545817, whole genome shotgun sequence.
ACCESSION   DAOPTR010001123 DAOPTR010000000
VERSION     DAOPTR010001123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80110_length_1310_cov_3.545817"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(335..>1310)
                     /locus_tag="PKI05_16230"
     CDS             complement(335..>1310)
                     /locus_tag="PKI05_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOA53801.1"
                     /translation="GAETGLKHVMERVELVAMSDAPVLLLGETGTGKELIARTIHQRS
                     PRAKGPFLRVNCGAIPRELIDSQLFGHERGSFTGAVETHQGWFERANHGTLFLDEIGE
                     LPLDAQVRLLQVLQDGTFERVGGCHPITVDVRVVAATHRDLQEMVRQGQFREDLWYRL
                     AVFPIYLPPLRERREDIPALARHFAQRAAVRFGLPLQLPTEEDIRALLSYHWPGNIRE
                     LAAVIDRAAILGNGRGLEVLKALGVTAVPSVSKTETLQPQEGDFPTLDEVMRRHIEEA
                     LRRCAGRIEGPRGAAALLGINPHTLRSRMRKMGICWAKFRTENGVSRS"
BASE COUNT          273 a          393 c          363 g          281 t
ORIGIN      
        1 cccgaacggt ttccaccggg aggtgctccg atttggcaac gtattccatt tcgggtgtga
       61 taattcccgc ccgggcggct tcaatctgag ttgacatttt ttcgacgatt ccctgacttc
      121 taaactcggt taacaacgaa accctcttcg gttaacggca agcagggccg actctttaat
      181 catattggat tccccaaaac gaacaaccaa gcacacagga agtttgctga ttccatcttt
      241 gggattttgg aaggcgtttt gtgggacgcg aaagctggtc ttgccgtaag tcccttcaac
      301 gtgagggttt gttttaagat gatcagatag agattcatga ccttgaaacc ccgttctcgg
      361 tacggaattt ggcccagcag attcccatct ttctcatgcg tgatcgcagg gtgtggggat
      421 tgattcccaa tagggcggcc gccccgcggg gcccttcgat ccgacccgca caacgccgca
      481 atgcctcctc gatgtgcctt cgcatcacct catccagtgt aggaaagtca ccctcctgtg
      541 gctgcagagt ctccgtcttg gacacgctgg gcaccgcggt gacgcccagg gccttcagga
      601 cttccagccc gcgaccgttg ccgagaattg cagcgcggtc gatgaccgcg gccaattcac
      661 ggatattgcc cggccagtgg taggaaagca acgcccgaat atcctcctcg gtgggtagct
      721 gcaacggaag cccaaaccgc acagcggccc gctgggcaaa atgtcgggct aaggcgggga
      781 tatcctcccg tcgctcccgg aggggaggca aatagatggg gaagacggcc aaccggtacc
      841 agaggtcttc tcggaattgg ccctggcgaa ccatctcctg taagtcgcga tgagtggcgg
      901 ccacgacccg cacgtcaacg gtgatcggat ggcaccctcc cactcgctcg aaagtcccgt
      961 cttgaagcac ctgaagcaag cggacttggg catcgagggg taattccccg atctcgtcca
     1021 agaataacgt cccgtgattg gctcgctcga accacccttg atgggtttct accgccccgg
     1081 taaagctacc ccgttcgtga ccaaagagtt gtgagtcgat caattcccga ggaatcgcgc
     1141 cgcagttcac acggagaaag ggccctttag cgcgggggga ccgttggtgg atggttcggg
     1201 caatcagttc cttgcccgtg cctgtctctc ccagaagaag aacgggcgca tccgacatcg
     1261 ccaccagttc cacccgctcc ataacgtgtt ttagccccgt ctcggccccg
//
LOCUS       DAOPTR010001124         1308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_80268_length_1308_cov_3.390263, whole genome shotgun sequence.
ACCESSION   DAOPTR010001124 DAOPTR010000000
VERSION     DAOPTR010001124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80268_length_1308_cov_3.390263"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PKI05_16235"
     CDS             complement(<1..815)
                     /locus_tag="PKI05_16235"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOA53802.1"
                     /translation="MRIDNQSSFCLAAAICSHLFVLAMVFATWGLWTTVAYGAEDWPQ
                     FHGPNRDNICTETDLLESWPEGGPKLLWQLEGLGRGYSTVSIAGGKLFTMGDRRTEDG
                     EAQFVLAFDLAIRKELWATRIGLPHRDGGPRCTPTVDGPRVYAIGTDGDLVCLETATG
                     KVIWKKNFAQDFGGRMMSGWRYSESPLVDGEKLVCTPGGKNALIVALNKETGDVIWRC
                     DPGNLGPQGQDGAGYASLIPAVICGVRQYVTIVGRGAVSVDAETGRLLWNYNR"
BASE COUNT          302 a          423 c          320 g          263 t
ORIGIN      
        1 attcgattgt aattccacaa cagtcgcccg gtctccgcat ccacactaac ggccccccgc
       61 ccaacgatcg tcacgtactg ccgaacccca caaatgaccg ccggaataag agatgcataa
      121 ccggcgccat cctgcccctg aggtcccaaa ttgcccggat cacatcgcca aatgacatct
      181 cccgtctcct tattgagggc aacgatcaag gcattctttc cccctggcgt acagaccaat
      241 ttttcgccat ccactagggg cgattcactg taacgccaac cggacatcat cctcccgcca
      301 aagtcttggg caaagttctt tttccaaatg actttgccgg tcgccgtttc gagacaaacc
      361 aggtcaccgt ccgtgccaat ggcgtacacc cgcggaccat cgacggtcgg cgtgcaccga
      421 ggaccaccgt cccgatgcgg caggccaatg cgcgtggccc aaagctcttt tcgtatcgcc
      481 aggtcgaacg ccaggacaaa ctgggcctcg ccatcctcag tcctccgatc ccccatggtg
      541 aagagctttc cgcccgcaat ggacaccgtc gaatagccac gaccaaggcc ttccagttgc
      601 caaagtaact tcggcccacc ttccggccaa gactccagta ggtcggtctc ggtgcagata
      661 ttatcgcgat tgggaccgtg gaactgcggc cagtcttccg ccccataggc aaccgtggtc
      721 caaagacccc acgtggcaaa gaccatggcc aacacaaaaa gatgcgagca aatcgccgcc
      781 gccagacaaa acgacgattg gttgtcaatt ctcatgacag actccatcga ttcgaggaac
      841 catacctaag aactcccgcc cctgtggata tgtcaagtat aagcaacagc gcagccgttt
      901 gccattcgtg gttgattgcg atcttgtgac gctgccggct gtggggccat gggtttgtca
      961 ctcgcgaaaa atcgcagccc ccgactagac tcgctgcaca tgcgtcgttc ggacgagtga
     1021 ctttcggtat gcagaccaag atgagcgtca atcattgccc cgagacgcta gtgtaagctg
     1081 gcgatggttc ataaaggacc acgcgtgttc catttccact aggcgcgacc cacaggctgc
     1141 ctctagagtt tgcctctaca gtttcgtccc ctcatgaccg cgcgtgacca ccagggtaaa
     1201 tgctggtcca agatcaaact tggagaggcg cacatcgtgg tggccaagtt gtttcgagcc
     1261 gccggaccaa accagtggca cacaagtcgt attacctggc ccgtgctc
//
LOCUS       DAOPTR010001125         1303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_80678_length_1303_cov_4.507212, whole genome shotgun sequence.
ACCESSION   DAOPTR010001125 DAOPTR010000000
VERSION     DAOPTR010001125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80678_length_1303_cov_4.507212"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /gene="cpaB"
                     /locus_tag="PKI05_16240"
     CDS             <1..1117
                     /gene="cpaB"
                     /locus_tag="PKI05_16240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HOA53803.1"
                     /translation="ETVTVNPRKTKTIVVAKYNLPQYARITEDYIDEVQVPADKVPEG
                     ALTLKSRALYRLAKTTILAGQPIRDDDLYAVGEVPLLSERLPPGYRAVTLAVDAKAAV
                     NGMIQPESLVDITLTVKNDRPEIGGLATLTLLRRVRVLATSRNRFPASEDRPGDLRNI
                     TVAVTPEQANKLILAEKYGTLSVVLCSKLEEETDEATLEDIMASKGEVPVIPTSNPVT
                     RADSTLSEPSNDLVNIYSLLGISPLTPEPMPEQPVTKTAQIWRGGNVEEVTFVEWQIR
                     EAENATLVAEGKTPQPFTPGTLTSSPKTTKTHDAEAEDCPECAAKRAKEAAARSVGEM
                     PVPTPASQLVPRNDQANHLPGGYGRVVTVPVRGQLAN"
BASE COUNT          332 a          363 c          355 g          253 t
ORIGIN      
        1 cgagaccgtg actgtgaacc ccagaaagac aaagactatc gtggtggcca agtacaattt
       61 gccccagtat gcaaggatca ccgaagacta tattgacgag gtccaggtcc ctgcagacaa
      121 ggtcccagaa ggtgccctga cgctcaagtc tcgcgctttg tatcggttgg caaaaaccac
      181 aatccttgcc ggccagccca ttcgcgatga cgacctctat gcggtaggcg aggtgccgct
      241 tctctccgag cgacttcctc ctggatatcg ggcggtaacc ctggcagtgg acgccaaagc
      301 ggccgtcaat ggcatgattc agccggagag tttggtggat atcacgttaa ctgtgaaaaa
      361 cgacaggccg gagattggcg gtctagccac actgacgttg cttcgtcgcg tccgtgtgtt
      421 ggcgaccagc cgcaatcgat tcccagccag tgaagaccga cctggcgatt tgcgcaacat
      481 cacagtcgcc gtaacgcctg aacaggccaa taagctcatt ttggcggaaa aatatggcac
      541 gctcagcgtt gtcctctgca gcaaactgga ggaagagacg gatgaagcca cccttgagga
      601 cattatggct agcaagggag aggtcccggt catccccaca tcgaaccctg taacccgggc
      661 cgatagtacg ttgtccgagc cttccaatga cctcgtcaac atctattccc tccttggaat
      721 ttcacccctg acgcctgagc cgatgcccga gcaaccggtc accaaaacag ctcagatctg
      781 gcgaggtggg aacgttgagg aggtcacgtt tgtggaatgg caaatccgcg aagcggagaa
      841 cgcaacctta gtggctgaag gaaaaacccc acaaccattt actccgggaa ccttaacgag
      901 ctcacccaaa accacaaaga cccatgatgc ggaggcggag gactgcccag agtgtgcggc
      961 aaaacgggcc aaggaggcag cagctcgttc agtgggcgag atgccggtcc cgacgcccgc
     1021 atcgcaactg gtaccacgaa acgaccaggc caaccatctc ccaggtggtt acggccgagt
     1081 ggtcactgtc cctgtgcgtg ggcaactcgc taattgacag tccgcgcaac aggttactca
     1141 tcgtcaccgt gtttattgtt cagataaccg gatgaaagag tgatcggaaa caattgtccc
     1201 atacaatctc tattcgagtc atgggaaatc aggccatgat gagttttctc tatcgtcaaa
     1261 gtgataaaga tggaaatggg cgcaagaacg tcttgaccga ggc
//
LOCUS       DAOPTR010001126         1299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_81009_length_1299_cov_3.688103, whole genome shotgun sequence.
ACCESSION   DAOPTR010001126 DAOPTR010000000
VERSION     DAOPTR010001126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81009_length_1299_cov_3.688103"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PKI05_16245"
     CDS             <1..221
                     /locus_tag="PKI05_16245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53804.1"
                     /translation="DGKVYVGTRRGVFWTFAASREKQLLAEINLRDSIASTPVAANGV
                     LYVTTLTHLYAVQQGPDVSEKTSSGMAN"
     gene            complement(279..1112)
                     /locus_tag="PKI05_16250"
     CDS             complement(279..1112)
                     /locus_tag="PKI05_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOA53805.1"
                     /translation="MGNIYIGANLEFVRHADKSFEWGVQKAAELGYEYVEPMVHWGRE
                     LMSEAGYFHSVSMLDDPYRIRNTCEKAGVKISGLSAHCPLGRPEVSVEYLKQAIRYAA
                     ECGAPVVNTDEGPKQPWTTEEEDFVLMRYTLREATFVAERRGILIGLEPHQQYSKSPD
                     GLDRIAALVPSPVIGINFDTGNAYLAGEDPIAWLERVVDRLVHVHAKDISVEQSEAER
                     GKVTGTAVGCACGDGVIDWRKVIQVCRKAPRDIVFSVECGTVEQAERSIKYLKELLKE
                     V"
BASE COUNT          290 a          392 c          323 g          294 t
ORIGIN      
        1 cggatgggaa ggtttatgtc gggacacgtc gtggagtctt ctggaccttt gccgcatctc
       61 gcgaaaaaca acttttagcg gaaatcaacc ttcgagattc catcgcctcc acacccgtcg
      121 ctgccaatgg cgttttgtac gtcaccacat tgacgcactt atatgcggtc caacaaggtc
      181 ccgatgtgtc ggaaaagacg tcctccggaa tggccaacta atgggaaggg ccctccgcgg
      241 tgatcagttt cgcctcgtcc gcgtggccca tgtgaggatc agacctcttt caaaagttcc
      301 ttcaaatact tgatactccg ctcggcctgt tcgaccgtgc cacactcgac gctaaaaaca
      361 atatcgcgcg gcgcctttcg gcagacttgg atgacttttc gccaatcgat gacgccatca
      421 ccgcatgcgc acccaaccgc cgtccctgtc actttccctc gttcagcctc ggattgttct
      481 accgaaatgt ctttggcatg aacgtgaaca agtcggtcca ccacacgttc cagccaagcg
      541 atggggtcct cacctgccag gtaggcattg cccgtgtcaa aattgatccc gatcacgggc
      601 gaaggtacga gggcagcgat gcggtccaag ccatcagggc ttttgctgta ctgttgatgc
      661 ggttccaaac cgatcaaaat cccacgtcgt tccgccacga atgtggcctc acgaagggta
      721 tagcgcatca ggacaaaatc ctcttcttcc gtggtccacg gttgcttggg gccttcatcg
      781 gtattcacca ccggggcacc acactccgcc gcatagcgga tggcctgctt taagtattcc
      841 acactgactt ccggccttcc gagagggcaa tgggccgaaa ggccggaaat cttgaccccc
      901 gccttctcgc aggtattgcg gatgcgataa ggatcgtcca gcatggatac gctatggaaa
      961 taaccggcct cggacatcaa ctcgcggccc caatggacca tgggttccac gtattcgtag
     1021 cccaattcgg ccgccttctg gacgccccat tcaaacgatt tgtcggcatg tcggacgaat
     1081 tcaagattgg caccaatata gatgtttccc atcgcttgac cctcctcgga aaaagaggtc
     1141 cccaaactac gcaggcaatg cgttggttac gttgcggcca ttaaatcaca gatatccatc
     1201 ggccgcaagt aactgaatac cgagtgcccg cgcgatttgt tggagggcca cgcgctcgcc
     1261 atccctgcga atactatctc atgaaggtca attcatcgg
//
LOCUS       DAOPTR010001127         1296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_81260_length_1296_cov_3.555197, whole genome shotgun sequence.
ACCESSION   DAOPTR010001127 DAOPTR010000000
VERSION     DAOPTR010001127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81260_length_1296_cov_3.555197"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PKI05_16255"
     CDS             <1..439
                     /locus_tag="PKI05_16255"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOA53806.1"
                     /translation="FDRSTEPWHEGTYMVERVVDGDTLKLTNGETVRLMGIDSPETVK
                     PQSPVEPFGEEAAAFTRRLVQNNSFQVRLSFDSQRRDKYGRLLAYVWAGEYFLNEELV
                     RAGLARVETQYSYSPLMKERLILAETEAKAQHRGIWSLSNSDK"
     gene            823..>1296
                     /locus_tag="PKI05_16260"
     CDS             823..>1296
                     /locus_tag="PKI05_16260"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOA53807.1"
                     /translation="MLCRLRCYYVPLTVLLWVASNCPGSFGALPESSLYLVGVGPGDA
                     DLITLRAIEAIKKADLVFCTDGLEKEFADYLSGKQVITGYWRLFEFYGQDISQLEGEE
                     RARAEQLAKKRNEFIHLVRESVVQKKTVVILDSGDPLIYGPWAWCLEEFEDLKPVV"
BASE COUNT          285 a          329 c          365 g          317 t
ORIGIN      
        1 attcgaccgt agcacagaac catggcacga agggacctac atggtggagc gggttgtgga
       61 tggggacacc ctcaagttaa ccaacggaga aacagtccgc cttatgggga tcgattcacc
      121 ggaaaccgtt aaaccgcagt cgcccgtgga acctttcgga gaggaggcgg cggcgttcac
      181 gagaaggctt gtgcaaaaca attcctttca ggttcgtctg agctttgact ctcagcgacg
      241 ggacaagtat ggccgactgt tggcgtatgt ctgggctggt gaatacttcc tcaatgagga
      301 gctggtccgt gccggcttgg cccgcgttga aacccagtat tcgtattcac cgctcatgaa
      361 ggaacgtctc atcctggccg aaacggaggc caaggctcag caccgcggga tttggtcact
      421 atcaaattcc gacaagtaac gggcttgtgg gtaacaaatt gttccgttcc gccatcttaa
      481 aatcgaggac ggtttcccca cagagtccgc ccgcaatagc aaccttatct tcgcactact
      541 ggcgtgcgaa tcgcatcttt gcaagtcagc agtgcttgga tacatacacg cccgcaagac
      601 attggcacag ccttcttcac tgccatcgca tatcggcacc ctcttgcgta ggtcgccgta
      661 tggtgttaca ttttctcgat gcgtaacatc gccgccatcg gctggtggac atatttcgct
      721 gcatttcttg tgcttgggcg attgtgacac cgttgtctaa gaattggcgc cgtcagttac
      781 cccattgtgg agttggtcta aagacgtgaa aggatcgcag ccatgttgtg ccgcttgaga
      841 tgctactatg tgcctctgac cgtcttactt tgggttgcga gcaactgtcc gggctcgttt
      901 ggggccttgc ccgaaagttc cctttacctt gttggtgtcg gtcccggcga tgcagacttg
      961 atcacgctgc gagccatcga agccatcaaa aaggctgacc tcgttttttg taccgatggg
     1021 cttgagaaag aatttgcgga ttatctctca ggaaaacagg tcatcacggg atactggcgg
     1081 ttgtttgagt tctacgggca agatatcagt cagcttgagg gggaagaacg tgctcgcgcg
     1141 gagcagcttg ccaagaaacg aaatgagttc atccatttag tgcgggaaag cgtcgtgcag
     1201 aagaaaaccg tggtcattct cgatagtggg gatcccttga tctacggccc ctgggcatgg
     1261 tgccttgagg agttcgagga tctaaagccc gtcgtc
//
LOCUS       DAOPTR010001128         1295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_81354_length_1295_cov_3.837903, whole genome shotgun sequence.
ACCESSION   DAOPTR010001128 DAOPTR010000000
VERSION     DAOPTR010001128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81354_length_1295_cov_3.837903"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            381..>1295
                     /locus_tag="PKI05_16265"
     CDS             381..>1295
                     /locus_tag="PKI05_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007332133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOA53808.1"
                     /translation="MLEELQKVIVGQTEVIEQLLAAIFTRGHCLLVGVPGLAKTLMVN
                     TLAKILDVQFKRIQFTPDLMPSDITGTNVLEEDEHGKRYFRFVEGPVFTNILLADEIN
                     RTPPKTQAALLQAMQEREVTVGQQTYPLPEPFLTIATQNPIEQEGTYPLPEAQLDRFM
                     FNIKVDYPTLEEEERILAATTRSEKVEVRKILSGRAILNLQKLVNSVAVSPYIVQYVA
                     RLVRATRPRDESAPQFVREFIDWGAGPRAGQFLIQGGKALAAMDGRFAVSIEDVRKLA
                     IPVLRHRISTNFQAQAEGLTSDDIILRLV"
BASE COUNT          303 a          323 c          363 g          306 t
ORIGIN      
        1 aaaacgacaa agcaattcgc gttctcggtt ttctgcgcca gaaaaacatc gacgccacac
       61 cggctctttc attttggtag gtcggcgtgc acccgagcaa cggtaccgga agaatttcag
      121 gatttggcag gtacgtaagg agcacaaggt gttcggttga ggcaaaccgt aacggtccaa
      181 agaattgacc tttctcatca tctatctcgt cccttccctt tggcatctta taatgaagca
      241 tggatgaggg aaaagagatt ccctgctcca taggaaaagc gcgaagtcgt ggcggtcttt
      301 cttgctgagg tgaaccattg tccacaaaac cccgcagtct tggtgacgtc cttcaggaat
      361 tcagccacta ccgccggctg atgctcgagg aactccagaa ggtgattgtt ggccagacgg
      421 aggtgatcga gcaactcttg gcggctattt tcacgcgcgg tcactgtctt ttggtggggg
      481 tgcctggttt ggcaaaaacc ctcatggtca acacactggc caagattctg gacgtccaat
      541 tcaaacggat tcagtttacg ccggatttaa tgccctcgga catcactggg acgaatgtct
      601 tggaggaaga tgagcacggc aagcggtatt ttcgctttgt ggaaggcccg gtgtttacca
      661 acatcctgct tgcggacgag atcaaccgta ccccaccgaa aacgcaggcc gccttgctgc
      721 aagcgatgca agagagggag gtgaccgtcg gtcaacagac ttaccctctg ccggaaccgt
      781 ttttgaccat cgccacccaa aacccgatcg agcaggaagg gacctacccc cttcccgagg
      841 cacaactcga tcgttttatg ttcaacatta aggttgacta tccaacgttg gaggaggaag
      901 agaggatttt ggccgcaaca acgcgcagtg agaaggttga ggttaggaag atattatccg
      961 gcagggcgat cctcaatctt cagaagttgg tcaattcggt tgcggtaagt ccttacattg
     1021 tccaatacgt tgcgcggtta gtgcgggcca cacgtccccg cgatgagtcg gcgccacagt
     1081 ttgttcgtga gtttattgac tggggagcgg ggccgcgggc gggtcagttt cttattcagg
     1141 ggggaaaagc cctcgccgcg atggatggtc gctttgcggt ttccattgag gatgtgcgaa
     1201 aattggcaat tccggttttg cggcatcgga tcagcaccaa ttttcaggcc caggcagaag
     1261 gactgacaag cgatgacatt attcttcgtc tggtc
//
LOCUS       DAOPTR010001129         1294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_81451_length_1294_cov_3.240517, whole genome shotgun sequence.
ACCESSION   DAOPTR010001129 DAOPTR010000000
VERSION     DAOPTR010001129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81451_length_1294_cov_3.240517"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="PKI05_16270"
     CDS             complement(<1..1024)
                     /locus_tag="PKI05_16270"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA53809.1"
                     /translation="MSTHSSRQGREMKKGTSRRDFLKTTGVAGAGALTGLSLSRFVHA
                     SGQDPILKIALIGCGGRGTGAAANALEADPRTVLVAMADAFEDRLNSSLAALKKNHGD
                     RVQVTPETSFVGLDGYKKAIACDVDVVLLCEPPHFRPMHLRAAVEAGKHVFAEKPVAV
                     DAPGYRSVLESTEIARQKNLCIVSGLCWRYHKPTIETVKRIQDGAVGDIIVIQETYNT
                     GHIGGRDRDPNLTEMQFQLRNWYCFTWLSGDHNVEQHIHSLDKALWVMHDEPPAVAWG
                     LGGRQVRTEMPRYGNIYDHHAVCYEYANGVRVYSYCRQHANCWNDTTDHVFGTKGRAV
                     ILNKWQI"
BASE COUNT          268 a          379 c          367 g          280 t
ORIGIN      
        1 cgatttgcca cttgttgagg atcacagcgc ggcctttggt gccgaagacg tggtccgtgg
       61 tgtcgttcca gcagttagca tgttgacgac agtaagaata cacgcggacg ccattggcgt
      121 actcataaca cacggcgtgg tgatcgtaga tgttgccata gcgaggcatt tccgtccgga
      181 cctgtcgtcc acccaatccc caagctacgg ccggcggctc gtcgtgcatc acccataggg
      241 ccttatcgag actgtggatg tgttgctcga cgttatgatc acctgacaac catgtgaagc
      301 agtaccaatt gcggagctgg aattgcatct ccgtcaaatt gggatcacgg tcgcgcccgc
      361 cgatgtgtcc ggtgttataa gtttcctgaa tgacgataat gtcacccacg gcgccatctt
      421 ggatacgttt tacagtttcg atggtgggct tgtggtaccg ccagcacaaa ccggagacga
      481 tgcaaaggtt tttctgtcgg gcaatctccg tgctttccag gacggagcgg taacccgggg
      541 catccacggc cacaggtttc tccgcgaaga cgtgttttcc cgcttccact gctgcccgaa
      601 ggtgcatcgg tcggaagtgt ggcggctcgc acaacaggac gacatcgacg tcgcaagcga
      661 tggctttctt gtaaccatca agcccaacaa acgacgtctc aggggtgacc tggacccgat
      721 caccgtgatt ctttttcagt gccgcgaggc tgctattgag gcggtcttca aaggcgtcgg
      781 ccatggcgac caggaccgtg cggggatcgg cttccagggc gttggcagcc gcacccgttc
      841 ctcgaccgcc gcaaccaatg agggcaatct tgagtatcgg gtcttgcccc gacgcatgga
      901 caaatcggct caaggagagg cctgtcaagg caccggcgcc ggcaacacct gtggttttta
      961 gaaagtcacg ccgtgaggtc ccttttttca tttcacggcc ttggcgggag gaatgcgtac
     1021 tcatcgcttc gtcctttcct ggcaggggca acactgaagg ttttagatag cgaaaacaac
     1081 ctccaacaac tcgcgagcat gattgtcgtg gctgtagtgc caccgacgac cgatcccatc
     1141 atactccccg accggtgccg cgccaacatc tggcttagag cctatcccaa aattccctct
     1201 ccctctggga gaggctcagg gtgagggcaa gcgatcgtcg gagccgatcg agaaaaccag
     1261 cggggattcg agccaccctc accccgcgac ccct
//
LOCUS       DAOPTR010001130         1294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_81465_length_1294_cov_2.596449, whole genome shotgun sequence.
ACCESSION   DAOPTR010001130 DAOPTR010000000
VERSION     DAOPTR010001130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81465_length_1294_cov_2.596449"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1294)
                     /locus_tag="PKI05_16275"
     CDS             complement(<1..>1294)
                     /locus_tag="PKI05_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOA53810.1"
                     /translation="EMQTGEGKTLTATLPMYLYALAGRGCHLATVNDYLARRDAEWMH
                     PIYELLGLSVGVIQTQMSQAERRRAYAADVTYGTAKEFGFDFLRDRLLLRRIREGQTD
                     LIGAMLEGNHSEMDEKPVQREPFFALVDEADSILIDEARTPLIISALPTEEERLAVEC
                     YKWAAQVAHEFVEDIDYEYDHEKKTVELTREGRRKARALPKPEAMASVGMFHIYEYVE
                     RAIKVARDFIRDRDYVVKDGEIVIVDEFTGRLAEGRKWRDGIHQAVEAKEGVEVTIAT
                     GQAARVTVQDFFLRYRHLAGMTGTAKDSAWELRSIYKCPVIVIPTNRPVIRKRLPDLV
                     FGTQEAKWQAVVDEVCEMHAIGRPVLIGTRSIDKSEHLSRLLHERGIDHQVLNAHHIE
                     EEAKIVAEAGQRGRVTVSTNMAGRGTDIRLGPGVAELGG"
BASE COUNT          254 a          404 c          331 g          305 t
ORIGIN      
        1 gtccacccaa ttccgcaacg ccggggccga gacggatatc ggttccccgc cctgccatgt
       61 tggtggaaac cgtgacgcgc ccccgttgcc ccgcttccgc tacgatcttg gcttcttcct
      121 cgatgtgatg ggcattcaag acctggtgat caatgcctcg ctcgtggagc aacctggata
      181 ggtgctcaga cttgtcaatc gagcgggttc cgatgagcac aggccgtccg atcgcgtgca
      241 tctcgcagac ttcatcaacc accgcttgcc atttggcctc ttgcgttcca aagaccaagt
      301 cgggaagccg tttgcgaatc accggccggt tcgtcggaat gacgatcacg ggacacttgt
      361 aaatacttcg caattcccat gcagagtcct tggctgtacc cgtcatccct gccaaatggc
      421 ggtagcgcaa gaagaaatcc tgcacggtaa cacgggcggc ctgccccgta gcaatggtga
      481 cctcaacgcc ttctttggcc tccacggcct ggtggattcc gtcccgccat tttctccctt
      541 ctgcgaggcg tccggtgaat tcgtcaacga tgacgatttc gccatccttg accacgtagt
      601 cgcgatcgcg gataaagtcc cgagcaacct taattgcccg ttccacatac tcataaatgt
      661 gaaacatccc caccgatgcc attgcctcag gtttggggag cgcgcgggcc ttgcgacggc
      721 cctcgcgggt tagttctacc gtttttttct catggtcata ctcgtagtcg atatcttcaa
      781 cgaattcgtg ggctacctgc gcagcccatt tgtagcactc caccgccagc ctttcttcct
      841 ccgtgggtag cgcgctaata atcagcgggg tccgggcctc gtcgatcaag atgctgtcgg
      901 cttcatccac gagggcgaaa aacggctcac gttggaccgg tttctcatcc atctcgctgt
      961 ggtttccctc gagcatggca ccaatcaggt cagtttgtcc ttcccgtatc cgccgcaaga
     1021 gaaggcgatc gcgcaaaaag tcgaagccaa attccttagc cgttccgtaa gtcacatctg
     1081 cggcatatgc ccgacgtcgc tctgcttgcg acatctgtgt ttgaatgaca cccacagaaa
     1141 gccccagtaa ctcgtaaatc gggtgcatcc actcggcgtc gcgcctcgcc aaatagtcat
     1201 taacagtggc caggtgacaa ccgcgaccag ccagggcgta gaggtacatt ggtagtgttg
     1261 ccgttagcgt cttgccttcg cccgtttgca tttc
//
LOCUS       DAOPTR010001131         1294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_81476_length_1294_cov_2.415658, whole genome shotgun sequence.
ACCESSION   DAOPTR010001131 DAOPTR010000000
VERSION     DAOPTR010001131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81476_length_1294_cov_2.415658"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..1224
                     /locus_tag="PKI05_16280"
     CDS             91..1224
                     /locus_tag="PKI05_16280"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53811.1"
                     /translation="MSSANDAKDRLEKIHYDLLILDLLLPQWPEGDPEVRHSMDILLE
                     LHEGDNLQRPGRILGITSDKTVAGEVTAKFDEWTWTVVQYSESNDEWIGRIVNCVRYI
                     QTEWKQSGTERPVHQVDLAVVCALEKPELEEVLKLPWNWTASRPLDDVTFVHDGSFTV
                     AGRTITVSATFAPRMGMVSSALRSSAVITLLRPRLIAMCGICAGVTGKVNFGDILLAD
                     PAWDFQSGKRVRDKENTQFSIAPHQLHTPALVRTHVEQLRGDKDAMTKIAAKFDGEDS
                     SIIPKIVIGPVASGSAVLADGEVVNEIKSQHRELVGVEMEIYGMYAAAYGASQPQPLP
                     FALKAVCDFADPDKKDKHQRFAAYTSANVLRLLMERFGPRLLG"
BASE COUNT          309 a          306 c          351 g          328 t
ORIGIN      
        1 attttgattg ccgacgacta agctcgacgc tatggacgtc tgacgagtcc gctagaaggg
       61 ctcggggtaa aacgcggtga agttgatatt gtgtcaagtg cgaatgacgc aaaggatcgt
      121 ctcgaaaaaa tacattatga cttactgatt ctcgatctct tgctcccaca atggcccgag
      181 ggcgatccag aggtccgtca ttcaatggac atccttctcg agttgcatga gggcgacaac
      241 ctacagagac caggccgcat tctcggcatt acttctgata agaccgtggc cggtgaggtt
      301 acagcgaaat ttgatgaatg gacttggacg gtcgtccagt attcagaaag caatgatgag
      361 tggatcggtc gaatcgttaa ctgtgttcgt tatattcaga ccgagtggaa gcaatctgga
      421 acggagcggc cagttcacca agttgatttg gctgtggttt gcgcccttga gaagccggag
      481 ttggaagagg ttttgaagct tccatggaac tggactgctt ctcgccctct ggatgacgtt
      541 acttttgtcc acgacggctc tttcacggtt gccggtcgaa ctataactgt cagtgcaacc
      601 ttcgcgccgc gaatgggcat ggtttccagt gcgctaagaa gctctgcagt tatcaccctt
      661 ctaagacctc gtcttatcgc tatgtgcggc atttgcgcag gcgtgactgg caaagtgaat
      721 tttggagata tcctcctggc cgacccggct tgggactttc aaagcggtaa gcgggtacgg
      781 gacaaagaaa acacccagtt ttcgatcgcg ccgcatcaac tccacacgcc agccttggtt
      841 cgaacgcacg tcgaacagct ccgtggcgat aaggatgcaa tgacaaagat tgccgcgaaa
      901 tttgacggtg aagattcttc gattatccca aagattgtga ttggaccggt tgcatctggt
      961 tctgccgtgc ttgccgacgg ggaggttgtt aatgaaatca agtcacaaca tcgagagcta
     1021 gtcggtgttg agatggaaat ctacgggatg tatgcggcag cttatggagc gtcccaaccc
     1081 cagcctctcc cttttgcact aaaggccgtc tgtgactttg cggatccaga taaaaaagac
     1141 aagcatcaga ggtttgcggc ctacacgagc gctaacgttc ttcgtctatt gatggagcgg
     1201 tttgggcctc gattgcttgg atgacctcaa ctgggcactt cgaaaaaccc ctgatttcac
     1261 cgaaatcact gttgtaagtt gttgatttag cgat
//
LOCUS       DAOPTR010001132         1292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_81646_length_1292_cov_2.863379, whole genome shotgun sequence.
ACCESSION   DAOPTR010001132 DAOPTR010000000
VERSION     DAOPTR010001132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81646_length_1292_cov_2.863379"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="PKI05_16285"
     CDS             <1..749
                     /locus_tag="PKI05_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOA53812.1"
                     /translation="LLHGRPAGRRAAAKALAGFHGVEANALTLRAMQDPDPGVQEAII
                     PQLRQRGILGALPYIISKLESPHPMVRRAARKSLREFTFTRFLQVWDMLPPEVRRETG
                     VLVKRVDPRTTLLLRAELLSTFRLRRLRALEIAQELELVPRLESSIICLLRDDDPAIR
                     AEAAAALAHTDSAASYQALLETLNDANVFVRNIAEVTLEKRRRRMESQEQGQKNAPSG
                     ESQPRQPQPSGIISDETNVSHLDTSPTTTS"
     gene            746..>1292
                     /locus_tag="PKI05_16290"
     CDS             746..>1292
                     /locus_tag="PKI05_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53813.1"
                     /translation="MTSPTMRRFTQALLARAIWRLGHVALVQAVAWVITTFLVYGSEA
                     AAYGQGRTPLEILHREFQRRATGSDTDHTALLWGLAVFVGLVAVLMIIAWVINYFQQR
                     RPYFSHTLLFLELCCVHRMRIKDILLLWKWTSREKIRPRSLIFVDPGLWQNKIDHNLL
                     NVTAGSIAAHTEWNRLYTQLFG"
BASE COUNT          287 a          370 c          348 g          287 t
ORIGIN      
        1 tgctcctaca tggacgaccg gcagggcggc gggccgcagc aaaggccctc gcgggcttcc
       61 acggcgtcga agcaaatgcc cttactctcc gggcgatgca agatcctgac cccggggtac
      121 aagaggccat tattccccaa ttgcggcaac gcgggattct aggagcgttg ccctacatta
      181 tcagcaaact ggaaagccct caccctatgg ttcgtcgggc ggctcgaaag agtcttcggg
      241 agttcacgtt tacacgattc cttcaagtgt gggacatgct tccgccggaa gtgcggcgcg
      301 aaaccggcgt ccttgtcaaa cgagttgatc cccgcacgac cctcctgctt cgtgcagaat
      361 tgctttccac cttccgttta cggcgtctcc gagccctgga aattgctcag gaacttgaac
      421 tggtgccccg attggagtcg tcaattatct gtctccttcg agatgatgac cctgcgatcc
      481 gggcagaggc ggccgctgcc ttggcccaca cggactcggc tgcgagctac caggcccttt
      541 tggagaccct caacgacgcg aatgtgttcg tgagaaatat tgccgaagtg acccttgaaa
      601 aacgccgtcg ccggatggaa tcgcaagaac aaggtcagaa gaatgcccct tccggcgagt
      661 cacagccccg gcagcctcag ccatcgggga tcatttcgga tgaaaccaac gttagccatt
      721 tggacacgtc acccacgaca acatcatgac aagccccact atgcgacgat ttacccaggc
      781 attactcgca cgcgcaatct ggcgattggg ccatgttgca ctggtccagg ctgtggcttg
      841 ggtaataacg acgtttttgg tctatgggtc agaagcggct gcctatggcc aaggcagaac
      901 gccgctagaa atcctccacc gggagtttca gcgaagagca acaggaagcg acaccgatca
      961 tacggcactc ttgtggggct tagcggtttt tgtcggcctg gttgcagtgt tgatgattat
     1021 cgcgtgggtc atcaactatt ttcaacaacg tcggccgtac ttcagccaca cgttgctttt
     1081 ccttgagctc tgttgtgtcc accgcatgag gatcaaagac attctgttgc tctggaaatg
     1141 gacatcacgg gaaaaaattc gtcccaggtc gctgattttc gtcgatccgg ggctttggca
     1201 aaacaagatc gaccacaatt tactcaacgt caccgcggga tcaatcgccg cacataccga
     1261 atggaatcgt ctttacacac agttgtttgg gg
//
LOCUS       DAOPTR010001133         1291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_81747_length_1291_cov_2.555016, whole genome shotgun sequence.
ACCESSION   DAOPTR010001133 DAOPTR010000000
VERSION     DAOPTR010001133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81747_length_1291_cov_2.555016"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PKI05_16295"
     CDS             complement(<1..462)
                     /locus_tag="PKI05_16295"
                     /inference="COORDINATES: protein motif:HMM:NF036998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOA53814.1"
                     /translation="MTIYHRAIGESPGLATDLYQLTMAQGYWQHGMAERRAVFQFFYR
                     RAPFKGRFLIACGTQAVLNFLQHWRFSDEDVSYLKTLVRPDGTPLFCAEFLDYLRRLV
                     FRGEVLGVPEGTIVFPNEPILRVQAPLLIAQLLETPLLNLIGFPSLVATKAA"
     gene            881..>1291
                     /locus_tag="PKI05_16300"
     CDS             881..>1291
                     /locus_tag="PKI05_16300"
                     /inference="COORDINATES: protein motif:HMM:NF022694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HOA53815.1"
                     /translation="MFRISLATILLLGIELTNYAVVFASEEASRIFGFSGPLTSRRWS
                     NLSWPSPWSCWTAPTGNVSLRVINEPGNPGEPVLHLRAVRGSFVLYQDLRRSPFPADR
                     YPHLSWRWKAIQLPTGASAHDSSRNDQVLQVYLAF"
BASE COUNT          318 a          361 c          344 g          268 t
ORIGIN      
        1 ggcagccttc gtggcaacca aggagggaaa accaatcagg ttaagaaggg gagtttccaa
       61 caactgggca attaataaag gggcctgaac ccgcaagatg ggttcgttcg gaaagacaat
      121 ggtcccctcc ggtacaccca aaacctcccc gcgaaaaacg agccttcgaa gataatcgag
      181 gaactcagca caaaacagtg gtgttccgtc tggacggaca agggtcttga gataagaaac
      241 gtcctcgtcg gaaaaccgcc agtgttgcag aaaattgaga acggcctgcg taccacaggc
      301 gatcagaaaa cgacccttaa agggtgcccg gcgataaaag aactgaaaca cggccctccg
      361 ctcggccatg ccgtgttgcc agtagccctg ggccatcgtc aattggtata aatccgtcgc
      421 taggccggga ctttctccaa tggctcggtg gtaaatcgtc atcgcagttt gcactcaact
      481 cttatccgac aatgtgtaat gggaaaaggt tctctctgtt ccgtctttgt cagagaccaa
      541 ccgcccgttc ccgcaaatgg ccttcgacgt aaccaaacca tccatctcgt cgtcttcggg
      601 agccacaacc ccatgacaca gagtgaatgg acgggcttgc tgggctaatc ccaaaacgag
      661 caaacatcgt cacgagcaac accacgtcca ttataggaac gcaatgtccc tccaagtcac
      721 ggggacggaa gaactcaagg atcattccgg gctcggttgt cgggcggtct tggaaactct
      781 aaaatgaggg gcaaaagcgg caatattcgg cacagccgag accacaccta atgaatccat
      841 actggcccaa ctggcaggat gagggcggcg ggagcctata atgttccgaa tctctctggc
      901 cacgatcctg ttgctcggca tcgaattgac gaattatgcc gttgttttcg ccagtgaaga
      961 ggcaagtcgc attttcggat tctctggtcc gcttactagc cggcgctgga gcaatctgag
     1021 ttggccatca ccctggagtt gctggacggc ccccaccggc aatgtgagtc tccgggtgat
     1081 aaacgagcca ggtaatccgg gcgagccggt cttgcacctt cgggcggttc gtggttcatt
     1141 tgtcctctat caagacctgc ggaggtctcc cttcccggct gatcgttatc cccacctcag
     1201 ttggcggtgg aaggccatcc aactgcccac gggggccagt gcacacgata gctcccgcaa
     1261 cgaccaagtc cttcaggtgt atttggcttt t
//
LOCUS       DAOPTR010001134         1291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_81756_length_1291_cov_2.271036, whole genome shotgun sequence.
ACCESSION   DAOPTR010001134 DAOPTR010000000
VERSION     DAOPTR010001134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81756_length_1291_cov_2.271036"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PKI05_16305"
     CDS             complement(<1..289)
                     /locus_tag="PKI05_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53816.1"
                     /translation="MVSQPFTVGYVELHRSGELQRRIEEALARLADCTLCPRQCHANR
                     LEDDPVGSYCGIGRHAWVSSAFPHHGEEPCLRGWNGSGTIFFSGCNLKCVFC"
     gene            738..>1291
                     /locus_tag="PKI05_16310"
     CDS             738..>1291
                     /locus_tag="PKI05_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOA53817.1"
                     /translation="MRIKLPRAFTLVELLVVIAIIGLLIALLLPAVQAAREAARRAQC
                     LNHLKQLGLATHNYATSWGCFPPSAIVDRSVQSTGNNVSWGIHGRLLAYLEQVPLGSR
                     INLVEPWDQQPAIDSVRIAVLACPSDPKAGQLRDPGGGKPRLYSTTYGFNLGVYFVYD
                     PVSDRGGDGISYPNSSLSWAAVTDG"
BASE COUNT          288 a          346 c          352 g          305 t
ORIGIN      
        1 gacagaagac acacttcaaa ttgcatcccg aaaagaagat cgttcccgat ccgttccagc
       61 cgcgaaggca gggttcttcg ccgtggtggg gaaatgcgct tgatacccag gcgtgccgac
      121 caatgccgca ataagacccc acaggatcgt cctctaggcg attggcatgg cattgtcgtg
      181 ggcacagcgt gcagtcggcc aatcgggcca acgcctcctc gattcgccgc tgcaattcac
      241 cggaacgatg aagctccaca taaccgactg taaacggctg ggataccata agaagcgttt
      301 acggtatagc atcaggggtc acgtatttgc cttccatgct cacttcatct ggcgcaggag
      361 gcaaaaaaca attggaacgc cagaatagtt acccactcct tattttccgt aagcgccggt
      421 catcacaaaa gacaggtgtg ccacttgata ggtgtggcgg cgagaacttt tcgactagcc
      481 tttccgcagg ataaaccatg atgcgcgatt cgacatcgtg tgaaacattg tgacatgcac
      541 tctcggctga acggttcccg acacggcgtc tttggtggca aaagacgcgg ttttgtgaac
      601 tctcggccag aacgaacccc cttgggagcg cgatctgctg tgctcaattg attcatctcg
      661 acggtaagtg gcatggcggg gaaagaggtc gcgctcccgg agcgtagtgc attagcagtt
      721 cggaaaggtt gaaggaaatg cgtatcaaac taccaagagc gtttaccctg gtagaacttc
      781 tggttgtgat cgctattatt ggcttgctca tcgccttgtt acttccggca gtacaggcag
      841 cccgagaggc agcccggcgg gcgcagtgcc ttaaccacct taagcagttg gggctagcga
      901 cacacaatta cgcaacttcc tggggatgct ttcccccaag tgctattgtt gatcggagcg
      961 tccagtcaac cgggaataat gtttcctggg gtatccatgg tcgtcttctg gcctaccttg
     1021 aacaggtgcc gttgggttct cgaatcaacc tggtggaacc ttgggaccaa cagcctgcga
     1081 ttgactccgt acggattgcc gttttggctt gtcctagtga tcccaaggcc ggtcaactcc
     1141 gcgatccagg cggtggcaaa ccacgactct attccaccac ctatggattc aatttgggtg
     1201 tgtatttcgt ttatgatccc gtttcggatc gtggtgggga cggcatttcc tatccaaact
     1261 ctagcctttc ctgggcggcg gttaccgacg g
//
LOCUS       DAOPTR010001135         1289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_81947_length_1289_cov_2.550243, whole genome shotgun sequence.
ACCESSION   DAOPTR010001135 DAOPTR010000000
VERSION     DAOPTR010001135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81947_length_1289_cov_2.550243"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..>1289)
                     /locus_tag="PKI05_16315"
     CDS             complement(170..>1289)
                     /locus_tag="PKI05_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53818.1"
                     /translation="NPWDTGVLTALSEACDQCGFHEAQLVYLKMALDANPKDVEMCRR
                     CAKTLAELGLYDQAIAMWHKVEQLRPNDTEASKAVADLAVRKTIEAGGYDETGESRRR
                     VEAAGGAVSTADALSRKDRLEMEIKRQPNNLTLYIELAELYVREDDYARAEQVMAKAY
                     EVTKDVTAKEKLEDIQLRRLRQAMADAEKQYRASPNDTTKAQLVAAQKALWAKELEVY
                     RDRVERFPNNLSFRFELGVRYQLNEMYEEAIKQYQMAQSDPRRKGVCLLNLGQCFEKI
                     GQHRLALKHFEDAIQEISDRDAENKKRALYAAAVLAYRLKDYRRAEQHASALAAMDYS
                     YRNVGELLDRIAKRLEAERSGSTEEAVEVELPPGQDED"
BASE COUNT          245 a          379 c          326 g          339 t
ORIGIN      
        1 tgatggaccg attgtgcgca cgcctcttaa tgttctgacg aagccgtttc tttgcgcttt
       61 tggtatttgg catatctcaa ccgtgacaac aaagaactcg ttcatctagc ccgacaccca
      121 tcagacggac gggtgtccct ttgatctacc gaaaatgcca ttatggaggc taatcctcat
      181 cctgtccagg gggaagctct acctcaacgg cttcctccgt ggaaccggac ctttctgcct
      241 ccagtcgctt ggcaattcgg tccaaaagtt cacccacgtt acggtaagag taatccatag
      301 cggccaaggc gctggcgtgc tgctccgctc ggcgatagtc cttcaagcga tacgccaaga
      361 ccgctgcggc gtatagggcc cgtttcttgt tttcggcatc ccggtcagaa atttcttgga
      421 ttgcatcctc aaaatgcttg agtgctagtc tatgctggcc aattttttcg aagcattggc
      481 cgagatttaa caagcagacg cctttcctcc gggggtcgct ctgggccatc tggtactgtt
      541 taattgcctc ctcgtacatt tcgttcaatt gataccggac gcccagttca aaccggaatg
      601 acaagttgtt ggggaaccgc tccacgcggt cgcgatagac ttccaattct tttgcccaca
      661 aggccttttg ggccgcgacc aattgggcct ttgtggtgtc attcggggag gctcggtatt
      721 gtttttcggc atccgccatt gcctgacgca accgacgaag ctggatgtcc tctagctttt
      781 ccttggcagt cacatctttc gtcacttcgt aggccttcgc catgacctgt tcggccctgg
      841 catagtcgtc ctcgcgaaca tagagttcgg ctaactcaat gtacaaggtg aggttgtttg
      901 gctgccgctt gatttccatt tccagccgat ccttacggct gagggcatcg gccgtgctta
      961 ccgcgccgcc cgctgcttcg acacgtcgcc gcgactcacc ggtttcatcg tagccaccgg
     1021 cctcgatcgt tttacgcact gccaagtcgg ccacagcttt ggaggcttcc gtgtcattgg
     1081 gcctcaattg ctcgacttta tgccacatgg cgatggcctg gtcatacaat cccaattcag
     1141 ccagtgtctt ggcacaccgg cggcacatct ccacatcctt ggggttggca tccagggcca
     1201 ttttgagata aacaagctgt gcttcgtgga aaccgcattg atcgcacgcc tccgacaagg
     1261 ccgtcaggac gcccgtgtcc caagggttg
//
LOCUS       DAOPTR010001136         1286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_82189_length_1286_cov_4.176279, whole genome shotgun sequence.
ACCESSION   DAOPTR010001136 DAOPTR010000000
VERSION     DAOPTR010001136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82189_length_1286_cov_4.176279"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="PKI05_16320"
     CDS             complement(<1..647)
                     /locus_tag="PKI05_16320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOA53819.1"
                     /translation="MAELGEVYTAGRVQIYIGDCRQILRRLPAESVQCCITSPPYWGL
                     RDYEHPDQIGAEETPEEYVRNLVSVFREVGRVLRADGTLWLNVGDAYARNGGTGLCGP
                     NAKVGNTRKLIQKRNCKVPPCWGLKDRDLMGLPWRVAFGLQADGWILRSHIVWIKKAP
                     MPESVRNRPSRATEDIFLFAKNPKYYYNNQAVRERTGANLKNYWVLGPEPCDLEHP"
     gene            complement(990..>1286)
                     /locus_tag="PKI05_16325"
     CDS             complement(990..>1286)
                     /locus_tag="PKI05_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53820.1"
                     /translation="LSTFATLSHQQKPPEKGWDPPQVLVIPPFSEYSVFYHRLTIDRL
                     GRLFVSYDCWSTYWFYRNDHPGTRRALLVSSDQGRTWKLATNADLTQLVPLPSR"
BASE COUNT          312 a          387 c          305 g          282 t
ORIGIN      
        1 ggatgttcca aatcgcaagg ttctggcccc aggacccagt aattcttaag attggctccc
       61 gtgcgttcgc gaaccgcttg gttgttgtag tagtacttcg ggttcttcgc aaacagaaag
      121 atgtcctcag tcgcgcgaga aggccgattc cgaacgcttt caggcatggg cgcctttttg
      181 atccaaacga tatgagatcg caaaatccac ccgtcagcct gcaagccaaa agccacccgc
      241 cacggaagcc ccatcaggtc ccgatccttg aggccccagc acggaggcac tttacaattc
      301 cgcttttgaa tcagcttccg agtgttaccg actttggcat tcgggccaca cagccctgtg
      361 ccgccgtttc gcgcgtaagc atcgcccaca ttcagccata atgtcccgtc cgcccgaaga
      421 actcgcccca cctctctgaa caccgaaact aagtttcgaa catactcctc cggcgtctcc
      481 tcagcaccaa tctgatcggg atgttcataa tcccgtagtc cccaataagg tggcgacgtg
      541 atgcaacatt gcaccgactc cgccggcaaa cggcgcaaaa tctgccggca gtcgcctatg
      601 taaatttgaa cacgccccgc tgtatagact tctcccaact cagccattga gccatccatc
      661 aggcacacca cagaagttta agagtccagt ttcccttgct acattctcga cccaagaagg
      721 atgggacgtc aagccatctg aaaatctacg ttttcgggca attgcggcgc ctcaccgcca
      781 gtttacgttg gcagctcgaa tgaaggatta tcgataccgc gttaaaaaac acaccgtgtc
      841 ttgcaatagc ggcccatctc tcttgcgtct gtcgcagatg gaggatccct cgccgtcgtg
      901 gccgatcacg ttagaagcct tctcctcccc tgaaaccaaa gacccttgac tcatcacgtt
      961 gagagggcac tgccctttac cctctcagtt caccgggagg gcaacggcac aagttgggtc
     1021 aaatcggcat tggtggccag cttccaggtg cggccttgat cagaagagac gagcaatgcc
     1081 cgccgtgttc ccgggtgatc attccgataa aaccaatacg tggaccagca atcatacgac
     1141 acaaacaatc gcccaaggcg gtcaatcgtt aatcgatggt aaaaaacgct atattccgaa
     1201 aatggcggaa tgacaagaac ttgtggcggg tcccatcctt tttcgggcgg cttctgctga
     1261 tgcgaaaggg tcgcgaaggt acttaa
//
LOCUS       DAOPTR010001137         1282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_82629_length_1282_cov_1.776691, whole genome shotgun sequence.
ACCESSION   DAOPTR010001137 DAOPTR010000000
VERSION     DAOPTR010001137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82629_length_1282_cov_1.776691"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..>1282)
                     /locus_tag="PKI05_16330"
     CDS             complement(133..>1282)
                     /locus_tag="PKI05_16330"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HOA53821.1"
                     /translation="KGGRSYVALDITDTPTSSTTEATIANKVLWEFTHEDMGFSYGRP
                     NIVKTRKWGWVVIIAGGYNNILGTTNTSVPGKGVLFVLDPKSGTVLQKIYTNEGSVAN
                     PAGMAHQSGYVQDYTDMTIDFMYAGDLLGNLWRFDFSSPTNDVPNPTSPILHLRDSLG
                     NPQPVTTEPRVDPGTDLVRYVFVGTGRLLHTNDRNNTQQQTFYALRDGTVSDPYSSAA
                     NAANPLPSGITFPVQRNNMVQVDSLIEGVVRDSSKPMGWFYDLTGRRGTLAEQVVQHP
                     QVNEGVVSWIGTLMNQDKCNPGGESAAYAVKYGSAKSTFATIVNNVRVPIAFVSSTSG
                     LVGAKMVRYGSSIRLLGSYGNAYSPGFVGSQVGGFGDPRVLNWRIIGQ"
BASE COUNT          278 a          375 c          369 g          260 t
ORIGIN      
        1 gctgctctac cgactgagct acagcggcta acaacagccc actattctac atggccccca
       61 taggccgtgc aagcctgccc accgttggga aaaggcccgc accctgatgg atacggggca
      121 ggccaagcgt gctcattgtc cgatgatccg ccagttgaga acgcgcggat ctccaaaacc
      181 gccaacctga ctcccgacga aaccgggact gtaggcattt ccgtaggaac caagcagacg
      241 aatggacgag ccataccgaa ccatcttggc gcccaccaga ccggatgtgg atgaaacgaa
      301 ggcaatgggc acacgaacgt tatttacgat ggtggcaaag gtgctcttgg ccgaaccgta
      361 tttgacggca tacgccgcac tctcgccccc cgggttgcat ttgtcctgat tcatcagggt
      421 accaatccag gatacgacgc cttcgttaac ctgcggatgt tggaccactt gctcggcgag
      481 cgtaccgcga cgaccggtaa gatcgtaaaa ccagcccatc ggcttggacg aatcccgcac
      541 aaccccctcg atcaggctat ccacctgcac catattgtta cgctgcaccg ggaaggtgat
      601 gccgctggga agggggttgg cggcatttgc tgccgacgag taggggtcgg aaaccgtgcc
      661 atcccgcaag gcatagaagg tctgctgctg cgtattgttc cggtcgttgg tgtgcaggag
      721 tctccccgtt cccacaaaca catagcggac cagatcggtg ccgggatcaa cccttggctc
      781 ggtggtaacg ggctgcggat tgcccaggga atcccggaga tggagaatcg gcgaggtcgg
      841 gttgggaacg tcgttggtgg ggctggagaa atcgaaacgc cacaggttgc ccaaaaggtc
      901 acccgcgtac atgaaatcga tcgtcatatc ggtgtaatcc tgcacatagc cactctggtg
      961 tgccatgccg gccgggttcg ccacacttcc ttcgttcgtg taaatttttt gcaggacagt
     1021 gcccgacttg ggatccagga cgaagagcac gccttttccc ggaacactgg tattggtcgt
     1081 accgagaatg ttgttgtagc cgcccgcaat gatgaccacc caaccccact tacgcgtctt
     1141 cacgatgttg gggcggccgt agctgaaacc catgtcctcg tgggtgaatt cccaaagaac
     1201 cttgttggcg atcgtcgcct cggtggttga cgaagtcggc gtgtcggtaa tgtccagggc
     1261 aacgtaactc cgccctccct tg
//
LOCUS       DAOPTR010001138         1281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_82671_length_1281_cov_3.701468, whole genome shotgun sequence.
ACCESSION   DAOPTR010001138 DAOPTR010000000
VERSION     DAOPTR010001138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82671_length_1281_cov_3.701468"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1281
                     /locus_tag="PKI05_16335"
     CDS             <1..>1281
                     /locus_tag="PKI05_16335"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOA53822.1"
                     /translation="AYWGPNCRYHLENFRAALDEPGEWFLERDGTLYYYPYPDEDMQK
                     AEVIAPEVSEFIVIAGRPEEQKLVQDVTFRGLIFEHSQYVLPPEGHADSQAEVTIPAV
                     IMLDGAKNVTFEDCEIKHVGIYGVWFRRGCQECRIERCWLDDLGGGGIKIGEGRGADL
                     SRAEVHTKKIVCRNNIIHAGGRIHHGAIGVWIGHSGYNEVVHNDISDFFYTGVSVGWV
                     WGYKESLAHHNRIEYNHIHHLGWGVLSDMGGVYTLGVSPGTSVSHNVVHDIYSYDRYG
                     RGGWGLYNDEGSSHIVLRNNLVYRTKTGGYHQHYGRENRVENNIFALSMEGQIQRSRV
                     EDHISFFFERNIVYWKGGPLIVAGQVNDDNVRFTSNIYYNAAGEPIDFQGLTLEQRQA
                     KGWDLGSLIIDPRFIDPEKGDFRLAEDSPAKRVGF"
BASE COUNT          296 a          298 c          386 g          301 t
ORIGIN      
        1 gcgtattggg gacccaattg tcggtatcat ctcgagaact ttcgggctgc cctcgatgaa
       61 cccggcgaat ggttcctgga acgcgatggg acactctact attaccccta tcctgatgag
      121 gacatgcaga aggccgaggt gatagccccg gaggtaagcg agtttatcgt cattgcaggg
      181 cgtcccgagg aacagaagct ggtccaagat gttacttttc gaggtctgat ctttgagcac
      241 agccagtatg tgttgccccc ggaaggacat gccgactccc aggcggaggt taccattccg
      301 gcggtcatca tgctggatgg ggccaagaat gtcacttttg aggattgcga gatcaagcac
      361 gtaggaatct acggtgtgtg gttccgacgg ggttgtcaag agtgccgcat cgaaaggtgt
      421 tggcttgatg acctgggcgg gggcggcatc aagattggtg aaggccgggg cgcagacctc
      481 agtcgtgcgg aggtccatac aaagaaaatc gtttgtcgaa acaatatcat tcatgcgggt
      541 gggcggattc atcacggggc gatcggtgtt tggatcggcc acagcggtta taacgaggtg
      601 gttcacaacg acattagtga tttcttttac acgggcgttt cagtaggttg ggtctggggc
      661 tacaaggaat cgctggccca tcacaaccga atcgagtaca accacattca tcacttggga
      721 tggggtgttt taagtgatat gggaggtgtt tacacgctcg gggtttcgcc cgggacaagt
      781 gtcagccata acgttgtcca tgacatttac tcctacgatc gctacggtcg cggcggctgg
      841 ggactttata acgacgaggg aagctcgcac atcgtcctga gaaataacct agtgtatcgc
      901 accaagacag gtggttatca ccaacattac ggccgtgaaa accgcgtgga gaacaatatc
      961 ttcgccctga gcatggaggg ccaaattcag cgatcacggg tggaggacca tatttccttc
     1021 tttttcgaac gcaacattgt gtattggaaa ggcgggcctt tgatcgtggc tggtcaggtt
     1081 aatgatgaca atgtccgctt tacctcaaac atctattaca acgcggcggg agagccgatc
     1141 gactttcaag ggctaactct ggagcagcga caagccaagg gctgggacct tgggtcattg
     1201 atcattgatc cgcggttcat cgacccagaa aagggcgatt ttcgcctggc tgaggattcc
     1261 ccggcaaagc gggttgggtt t
//
LOCUS       DAOPTR010001139         1279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_82866_length_1279_cov_3.965686, whole genome shotgun sequence.
ACCESSION   DAOPTR010001139 DAOPTR010000000
VERSION     DAOPTR010001139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82866_length_1279_cov_3.965686"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..422)
                     /locus_tag="PKI05_16340"
     CDS             complement(126..422)
                     /locus_tag="PKI05_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53823.1"
                     /translation="MLKERSEISPSESLHREVSDPSRHIEMVAQRRFDKSGYPALRRL
                     SCHCDGRRLVIRGQVSSYYEKQIAQASLRDLSENLEIVNEAEVLWPTYRVEGTI"
     gene            complement(640..828)
                     /locus_tag="PKI05_16345"
     CDS             complement(640..828)
                     /locus_tag="PKI05_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53824.1"
                     /translation="MQDIKQRREEIAKQLRDPKTRDLVISRLKTLMGIPPNEPLPNGT
                     PIITTLIRLENERQHSGA"
BASE COUNT          276 a          352 c          343 g          308 t
ORIGIN      
        1 ggtaaagccg gcgactgacg cgccggcttt ccttttgttg atcgggcgcg agaggcggct
       61 tgcgcggctt ttgccgatgg ctttgtttgg gtcggcaagg ggatctaact ttgaaatgcg
      121 aggaactaga tcgtaccctc gacacgatac gtgggccaaa ggacctcggc ctcgttgacg
      181 atctcgaggt tttcggaaag gtcacgcaga ctggcctggg caatttgctt ttcgtagtaa
      241 ctgctgacct gtccacgaat cacgagccga cgaccgtcac aatggcacga gagtcgccgc
      301 aaggccgggt aaccgctctt gtcgaatcgc ctttgggcaa ccatttcgat gtgccgtgag
      361 ggatcactca cttccctatg aagcgactca ctaggggaaa tctcagaacg ttctttcaac
      421 atgacaccgc cccacctcag ggttgcaagc acgctccaag gatgccactc cgttttcacc
      481 ttcggcggag gtccctcttc cgccctctcc caaacccaag tgtcccagaa ggttgtcgcc
      541 ggttgacctt tctcggccaa acggcccata cagccccgag actttgtttc gcaaatgatc
      601 atttgcacgg gggaccagcg gtcaattccc cggcattcgt tatgctccag agtgctgtcg
      661 ttcgttctcc aatctgatca aggtggtgat aatcggtgta ccgttgggta acggctcgtt
      721 cggaggtatt cccattaatg ttttcagtcg tgaaatgaca agatcgcggg ttttcggatc
      781 acgcagttgc ttggcaattt cttcccgtcg ttgtttgatg tcctgcatcg ctaggaacct
      841 cccattcctt acgaggtcca gacatcacgc caacaaaccg ggcgaatttg gtgcgtaagg
      901 ccatcacggg ttcgaacacg tgccgacgga gttgaaagac aacaactccc aggtcgccaa
      961 ctacagccgc cggccgaact ggccaacgta atcgaatcgc ttaccatata cccctatcga
     1021 ctggtatcgc ggaaaagttt acctatttac tgtagttgcc atattttcct aaaggcgggc
     1081 acgtatcgcg tgcgggtgag tgggatattg ctccaccggg cgaaccaatg tgtcataaga
     1141 agaggcggtt tttgaggtga cccgattttc cctttccgct accatggagg gataggaaga
     1201 taccggtctg gcgattcttc ggcaatcacg gacgcaaatg agaagctacc cgttgtccca
     1261 acctgggagt gagattcga
//
LOCUS       DAOPTR010001140         1276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_83198_length_1276_cov_2.562654, whole genome shotgun sequence.
ACCESSION   DAOPTR010001140 DAOPTR010000000
VERSION     DAOPTR010001140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83198_length_1276_cov_2.562654"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..562
                     /locus_tag="PKI05_16350"
     CDS             68..562
                     /locus_tag="PKI05_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53825.1"
                     /translation="MSEHAEGPRWVDITFDCVPLRMVTRSDPPLDASPGFLALWDRVQ
                     EAIRRFGRHNSYYLFNGQCTFHLTNNPSKGTLSFAFEGCLLTDSEDKVATAFDVHTTL
                     RGDTCDWATEPIIRWFAESVREAVLVEFNHFIAHGDLEKTIQRLQKLEQEMEAKGGYV
                     GFGL"
     gene            complement(541..>1276)
                     /locus_tag="PKI05_16355"
     CDS             complement(541..>1276)
                     /locus_tag="PKI05_16355"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53826.1"
                     /translation="FRIGIIDDADYFNAEGANALLKTLEEPPPQALLILIGTSASKQL
                     PTIRSRSQIVRFRPLPVDILTELILEEGLSQNLDEARRLAQLSGGSLERARDLADPEM
                     WPFWVRWRAELSGISFDAVTWSQEFRTFVDNAGKEAAARRKRLYHILSFTANFLRDAI
                     TLLVGGHADDSTSAECQNFVRVIKEILGPDAVEVIQHCVETCLQACEDIERNANLTIL
                     IDDWITTLWHCLNRSRVASPQSPKPT"
BASE COUNT          291 a          342 c          338 g          305 t
ORIGIN      
        1 gcaatatacg aattgtgtta tctggggtat ttccaactga cttcttactg tccgggatgg
       61 ctttttcatg agtgagcatg cggaaggccc tcgatgggtg gacatcacat tcgattgtgt
      121 gcccctgcgg atggtcacgc gatctgatcc gccgcttgat gcctcgccgg gtttcctcgc
      181 cctttgggat cgcgtccaag aggccatccg aaggtttggt cggcacaatt cctactacct
      241 tttcaacggc cagtgtacct tccacctgac aaacaatccc tccaagggca cattaagctt
      301 cgcctttgag gggtgcttgt tgactgattc tgaagacaaa gtggcaacgg ctttcgatgt
      361 ccataccacg ttgcgagggg atacctgcga ttgggctacc gaaccaatca ttcgttggtt
      421 tgccgagagc gtgcgggagg cggtcttagt cgaattcaat cactttattg cccacggaga
      481 cctcgaaaag actatccagc ggctgcaaaa gctagaacag gaaatggaag ctaagggggg
      541 ctacgtcggc tttgggcttt gaggagaagc gactcgactg cgattcaagc agtgccacaa
      601 tgtggtgatc cagtcatcaa tgagaatggt gagattcgca tttctctcga tatcctcgca
      661 ggcctggaga cacgtctcca cacaatgttg gatgacttcc accgcatcgg ggccaagtat
      721 ctctttgata acgcgaacga agttctggca ttctgctgaa gtggagtcgt ctgcgtgccc
      781 ccctaccaag agtgttatcg catcgcgcaa gaaattagcg gtaaaagaaa ggatatgata
      841 aagccgcttg cgacgggcag cggcctcctt gcccgcgtta tccacgaacg tgcgaaactc
      901 ttgggaccag gtaacggcgt cgaatgaaat accgctcaac tccgcccgcc atcttaccca
      961 aaacggccac atttccgggt ccgccagatc tcgtgcccgt tcaaggctac cgccgctaag
     1021 ttgggcaagc ctgcgagcct cgtctaaatt ctgcgacagt ccctcctcca aaatcagctc
     1081 ggttaagata tccactggta atggtcggaa gcgcacaatc tgacttcttg accggattgt
     1141 cggcaactgt ttgctcgctg acgtgccaat gagaatcaac aatgcctgag ggggcggttc
     1201 ctccagcgtc ttgagcaagg cgtttgctcc ttccgcgttg aagtaatcag catcgtcaat
     1261 aatgccgatg cggaat
//
LOCUS       DAOPTR010001141         1275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_83289_length_1275_cov_2.845902, whole genome shotgun sequence.
ACCESSION   DAOPTR010001141 DAOPTR010000000
VERSION     DAOPTR010001141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83289_length_1275_cov_2.845902"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..>1275
                     /locus_tag="PKI05_16360"
     CDS             50..>1275
                     /locus_tag="PKI05_16360"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOA53827.1"
                     /translation="MLDPACGTMHFGLVAFDLFVAMYREEMQNAGKPGWPPKPPVASE
                     EDIPGAIIAHNLHGMDIDPRAVQLSALTLYLKAKTLNPKARISESRLACANVHMLDGD
                     NLKHFAEKMKLGPICQRILTALQARLKDSEQLGSLLRLEEEIRKLIAEERKRYEREGK
                     VPDLFGWHKEMFETEAGQREFWEVLEAQIGQALDAFAREHFGGVEQTYFVGETAKGLR
                     LLQLMARRYDVVVTNPPYMTSRNMNPTLKGLVAKHYAITKSDLYACFIQRCCEWLAHH
                     GRLGMIAQQSFMFISSYERLREYLRSSTAIEVVPHVGPRAFEEVTGEKVNTVLFVFRR
                     EPDPEARQKAVGTYFRLVKELDSEAKRKRFEKALANLRAGIDDPVVYRYRQGDFDAIP
                     GSPWVYWITPGLRKVF"
BASE COUNT          296 a          357 c          363 g          259 t
ORIGIN      
        1 ccctgccccc tgcccactac cgtctgtcaa aagtgtcaaa gagattactt tgctcgatcc
       61 tgcctgcggc accatgcact tcggtttggt ggcctttgac ctgttcgtcg ccatgtatcg
      121 ggaggagatg cagaatgccg gcaaacccgg ctggcctcca aaaccaccag tggcctccga
      181 agaagacatt ccgggcgcca ttattgccca caacctccat gggatggata ttgacccgcg
      241 ggccgtccag ctttcggcgc tcacactcta tctcaaggcc aagaccctga atcccaaggc
      301 ccggatcagt gaaagccgcc tggcctgcgc caatgtccac atgttggacg gcgacaatct
      361 gaaacatttc gcagaaaaga tgaagcttgg gcccatctgc caacgcatcc taaccgcctt
      421 gcaggcgcgc ctgaaggact cggaacaact cggttccctg cttcggctgg aagaagaaat
      481 tcggaaactc atagctgaag aacgtaaacg ctatgagcgc gagggtaaag tgcccgactt
      541 attcggttgg cacaaggaaa tgtttgaaac cgaagcaggg cagcgcgagt tctgggaagt
      601 gctggaggcc cagatcggcc aggccctcga cgcatttgca cgagagcatt tcggtggggt
      661 ggagcaaact tatttcgtgg gcgaaaccgc caaaggactg cgcctgctcc aactcatggc
      721 ccggcggtac gacgtggtcg tgaccaatcc accgtatatg actagcagaa atatgaaccc
      781 gacgctcaag ggcctggttg cgaaacacta tgccatcacg aaaagtgatc tatatgcctg
      841 cttcattcaa agatgttgcg agtggcttgc tcaccacggg agattgggca tgatcgccca
      901 gcagtcgttc atgttcattt cttcgtacga aaggttgcgc gaatacttaa ggtcaagcac
      961 tgctattgaa gtggtgcccc atgtggggcc tcgtgcgttt gaagaagtaa cgggagagaa
     1021 agtcaacacc gtattgttcg ttttccgtcg ggagcccgac cccgaggccc ggcaaaaggc
     1081 cgttggaacc tattttcggc tggtgaagga actggacagc gaggccaagc gcaagcggtt
     1141 tgaaaaggcc ctcgccaacc tgcgggccgg aatcgacgat cccgttgtgt atcgctaccg
     1201 gcagggcgac ttcgacgcca tccccggcag cccctgggtg tactggatta cgccggggct
     1261 gaggaaggtg ttcga
//
LOCUS       DAOPTR010001142         1273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_83445_length_1273_cov_4.302135, whole genome shotgun sequence.
ACCESSION   DAOPTR010001142 DAOPTR010000000
VERSION     DAOPTR010001142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83445_length_1273_cov_4.302135"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="PKI05_16365"
     CDS             complement(<1..903)
                     /locus_tag="PKI05_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53828.1"
                     /translation="MPESNENQEQNGQPKGHGGFNWRTFLLLILGIIVALVGMLAYRI
                     ATDSPPPPPDRGGEVQPGAPDGEPEVDTDAVRTEAWQRIQPRLEAADAKTRELTNQLV
                     HRIETFFDSRKEGARAFGEAALGWKSKWELIKSRDGHREFIAARFSEYIFTQDELAAL
                     LKEASDEYAQGLRAVENELLVQVRADLKNLPASALPAFANKNVLESRFDNIVATVAAD
                     VARDLQVDVGREITSFAVGEIVAVVVTKTLTAVATRLGISGAILGTGAASSWATFGAG
                     IVIGIAVDFLLDWIIGWFHDPAGDI"
BASE COUNT          249 a          392 c          370 g          262 t
ORIGIN      
        1 gatatccccg gccggatcgt gaaaccagcc gatgatccag tcgagcaaga agtcaaccgc
       61 gatgcctatc acaatcccag cgccgaatgt cgcccacgac gaagccgctc cggtgccgag
      121 aatcgcccct gaaatgccga gccgcgtcgc aacagcggtg agagtctttg tgaccacaac
      181 tgcgacgatc tcccccaccg caaaagaggt gatctcgcgt cccacatcga cctgcaaatc
      241 cctcgcgacg tcggctgcca ccgtggccac gatgttgtcg aaacgtgatt caagcacatt
      301 tttattcgca aaggccggga gagccgatgc tggcaggttt ttaaggtcgg ccctgacctg
      361 cacgaggagt tcgttctcga ctgccctcag tccttgggcg tactcgtcgg aagcctcctt
      421 gagtagggcg gcaagttcat cctgggtaaa aatatactcg gagaaacgtg ccgcgatgaa
      481 ctcgcgatgg ccatcgcggc tcttgatcaa ctcccatttg cttttccatc cgagagccgc
      541 ttccccgaag gcccgtgccc cctccttacg actatcgaaa aaggtctcaa tccgatgaac
      601 tagttggttc gttagctcgc gggtcttcgc gtcggcggct tcgaggcggg gttggatgcg
      661 ttgccatgct tccgtgcgga ccgcatcggt atcaacttcg ggttcgccat ccggcgcgcc
      721 aggctggact tcgccgccac ggtccggcgg tggcggcggc gaatcggtag caatccggta
      781 ggccaacatg ccgacaaggg caacgatgat accgaggatc aagagaagga aggtccgcca
      841 gttgaagccg ccgtgaccct ttggctgtcc attctgctct tggttttcgt tcgattcagg
      901 cattggtcca cccctttcgg aaagtcgcgg acagaactgc cgccatagac cagtgagatg
      961 ctaacccttg ccacagcatg cgtcaatcga gttttttgac tggccgaggt cgtcggccac
     1021 cgccatttct ggcttgcgcc gggtaattga ttggtgccca ccgcgcggca aatcgcctgg
     1081 ttcggcggat gtagtggtca aacgcaccgc tgcccacccg ccgggcgcaa cagcgccgcc
     1141 ctggctgcag atgtagatcg tggccaccgg gacacccgcc cacggatggc ataggaggag
     1201 aaaaggtgtc aggaaccttt atgctgctct ttattccgtt ggttccggat cgctgggccc
     1261 ggtgtgccgc gac
//
LOCUS       DAOPTR010001143         1272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_83546_length_1272_cov_3.760887, whole genome shotgun sequence.
ACCESSION   DAOPTR010001143 DAOPTR010000000
VERSION     DAOPTR010001143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83546_length_1272_cov_3.760887"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PKI05_16370"
     CDS             <1..715
                     /locus_tag="PKI05_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53829.1"
                     /translation="TPYGQYLLEDVINQRVPAKMLVFLAAWRLSKDERQELRKTTAGK
                     LKIWCYAPGYLAPGEEALAAMKELTSYDLKEISGVVAWAEPTEEGRRLGLTQGFGIRQ
                     PIRPLLAARDVRPEEVLAAYPDGSAAVVLRRLPDGPSLFVGVPNISSELLRLAAKEAG
                     VHLVAEEDAVIYRNHPFIVIHPVNDGQLTVRPGNNMPVWDYLTRESLGNGPTLSLEVK
                     AGHTRVLVCGEKITADMAR"
     gene            891..>1272
                     /locus_tag="PKI05_16375"
     CDS             891..>1272
                     /locus_tag="PKI05_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53830.1"
                     /translation="MSKSIFLTVIACGLLAGLICVASENPQKAFNSSQLSGPGNFFVA
                     PNGNDKWSGCLPAPNQDQSDGPWATLEHARDVLRKLRAERDFREGLPKTITVWVRGGV
                     YWLSRPLVFEAEDSGKSGSPVTYRA"
BASE COUNT          294 a          336 c          348 g          294 t
ORIGIN      
        1 aacgccttac gggcagtatc ttttggaaga cgttatcaac caacgggtcc ccgctaaaat
       61 gctcgtgttt ttggccgcgt ggcgactttc caaagacgag cgtcaggaac tgcgcaaaac
      121 gaccgcgggc aaactcaaaa tctggtgtta tgcgccagga tatctggcac ctggggagga
      181 agccctggcc gcaatgaagg aactcacgag ctatgatctg aaggaaattt ctggagtcgt
      241 ggcttgggcc gagccaacag aggaaggacg ccgactaggt ttaacgcaag ggttcggcat
      301 ccgtcagccc attcggccgt tgctggcggc acgggacgtt cgacccgaag aagtcttggc
      361 agcctacccc gacggaagtg ctgcggttgt cttacgcagg ttgcctgatg gtccatcgct
      421 ctttgtcggt gtcccaaata tcagcagtga attgcttcgt ctggcagcaa aggaagccgg
      481 tgttcacctc gttgccgaag aagacgccgt gatttatcga aatcaccctt tcatcgtgat
      541 tcatcccgta aatgacggcc agcttacggt gcgcccgggt aacaatatgc cagtttggga
      601 ttatctcacc cgcgaatcct tagggaacgg ccccacttta tccttagagg taaaagccgg
      661 gcacacccgt gttctcgttt gcggtgaaaa gatcacagcc gatatggcaa gatgatatgg
      721 ttgattgttt atacgcttgg ttgacaatta atattgcctc tgtacctcgg cttaaagccc
      781 ctgaccatgg cgcttacaca aatcctaata ccggaccaat caagcccggg aattaccaat
      841 tgctaagtta gcggtgccaa gatgcccttt ttcgtaggga ggtctcaact atgtcgaaat
      901 ccatcttttt gacggttatt gcgtgcggcc tacttgcagg tctgatctgc gtggccagcg
      961 aaaacccgca aaaggcgttc aatagttctc agctatccgg accgggaaac ttctttgtcg
     1021 cacccaacgg caacgacaag tggtccgggt gtttgccggc ccccaatcaa gatcaaagcg
     1081 atggcccctg ggcaactctc gaacatgcga gagacgtttt gcgaaaactt cgtgccgagc
     1141 gagatttccg tgagggcctt cccaagacga ttacggtgtg ggtccgagga ggtgtctatt
     1201 ggctttcccg accacttgtc ttcgaggcgg aagactcggg aaagagcgga agccccgtta
     1261 cctaccgcgc gt
//
LOCUS       DAOPTR010001144         1271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_83665_length_1271_cov_2.888980, whole genome shotgun sequence.
ACCESSION   DAOPTR010001144 DAOPTR010000000
VERSION     DAOPTR010001144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83665_length_1271_cov_2.888980"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="PKI05_16380"
     CDS             <1..649
                     /locus_tag="PKI05_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOA53831.1"
                     /translation="CGYGDVGKGCAQSLRAYGARVVVTEIDPICALQAAMEGYEVTTL
                     EEVAPRAQIFVTATGCCKVIRAEHMLAMPDGAIICNIGHFDVEIDVDWLMNKSGAKRT
                     TIKPLVDKFTFPNGKSIILLAEGRLVNLGCATGHPSFVMSNSFTNQVLAQIALWTEPE
                     KYPVGVHCLPKKLDEEVARLHLEQIGVKLTRLTPEQAAYLGVPVDGPFKPDYYRY"
     gene            710..>1271
                     /locus_tag="PKI05_16385"
     CDS             710..>1271
                     /locus_tag="PKI05_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOA53832.1"
                     /translation="MTKDNRSLLPKWGPVFLGLAGISEWDWIPMPEIQAFRGIRYDLG
                     HVGSLSDVIAPPYDVISPELQAFLYKKHPCNVVRLILNRIEPGDDEVNNRYSRAKRFY
                     QSWLAEGVLFREADPAIYVYYQEFEVDGRRYLRRGFMARVRLSAYGEGQVFPHEHTLR
                     GPRMDQLMLMTMCKANLSQVFGMYPDP"
BASE COUNT          272 a          352 c          364 g          283 t
ORIGIN      
        1 ctgcggctat ggcgacgtcg gaaagggttg tgcgcaatcg ctgcgggcat acggggccag
       61 ggtggtcgtt accgaaatcg accccatttg tgcccttcaa gccgccatgg aagggtacga
      121 ggtcaccacg ctcgaagaag tggccccgcg agcgcagatt ttcgtcacgg ccaccgggtg
      181 ctgcaaggtg attcgggccg aacacatgct cgccatgccg gacggtgcca tcatctgcaa
      241 tattggccac tttgatgtgg aaatcgacgt tgattggctg atgaacaagt ccggggccaa
      301 acgaacgacg atcaaacccc tcgtcgataa gttcacgttc cccaatggta agtcgatcat
      361 cttgttggcc gaaggccgcc ttgtgaatct aggttgcgcg accgggcatc cgtcgttcgt
      421 catgtcaaat tcgttcacaa accaggtcct tgcccagatt gccctttgga ccgagccgga
      481 gaaataccct gttggcgttc attgcttacc caagaagctc gacgaggaag tggcccggtt
      541 gcatcttgaa caaatcggcg tcaaattgac gcgactgaca ccggaacagg ctgcctattt
      601 gggcgttcct gtggacggtc cgttcaaacc tgactactac cgttactaat tgtggccgcg
      661 acaatcggcg acacggagcc atcttgtcgg tgggccgacc ataggacaaa tgacgaagga
      721 caatcggtcc cttttgccaa aatggggacc ggtttttttg ggactcgcgg gaatcagtga
      781 gtgggattgg attccgatgc cggagataca agcttttcga gggattcgct atgatctggg
      841 tcacgttggc tcgttaagcg acgtgatcgc accaccctat gacgtcatct cgcctgaact
      901 ccaggctttc ctctacaaaa aacacccgtg caacgtggtg cggctcatcc tcaaccgcat
      961 cgaaccgggt gacgacgagg tcaacaaccg gtattcgcgg gcaaaacgat tttaccaaag
     1021 ttggttagcg gaaggggtgc tcttccggga ggccgatccc gccatttatg tctattacca
     1081 ggagtttgag gtcgatggcc gccgctatct ccgccgcggg ttcatggccc gagtgcgtct
     1141 gtccgcgtac ggcgaagggc aagtttttcc tcatgaacac acgctccgtg gtccccgcat
     1201 ggaccagctt atgctgatga ccatgtgcaa ggccaacttg agtcaggtct tcgggatgta
     1261 tcccgacccc g
//
LOCUS       DAOPTR010001145         1270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_83758_length_1270_cov_3.010700, whole genome shotgun sequence.
ACCESSION   DAOPTR010001145 DAOPTR010000000
VERSION     DAOPTR010001145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83758_length_1270_cov_3.010700"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..>1270
                     /gene="nusA"
                     /locus_tag="PKI05_16390"
     CDS             16..>1270
                     /gene="nusA"
                     /locus_tag="PKI05_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOA53833.1"
                     /translation="MNPDELFRIVDAIHREKNIEKEVVFKSIEAALATVARKRYGDQA
                     DISISIDRKSGVISGRYGDQAIDPEQLSRIGAQAAKQVILQKIREAERDVLYEEYQEL
                     LDQVVTGTVQKVEPRAIIVSLPRTEAILPKSEQIPGETRHPNERIRAVVTEVKKAGTR
                     LRIVLSRIRPVFVVRLFEQEVPEIADGVIEIKSIAREPGYRTKVAVASVDQRVDCVGA
                     CVGLRGNRIRNIVEELSGERIDIIRWDDDLMTLIPNALQPAKVDEVILCQMLGRAIVL
                     VPEDQLSLAIGRWGQNVRLASKLCGWDIEIMTRDELDEQLEQAVLGFSSLEGVTPELA
                     DRLVGEGFLSYDDLSVIEPEDLMEMGGLTEEQVQHIIAQAEAKAEEAEQLAREEKRRQ
                     RQLEREREAALAEGVTSEVPPSAESG"
BASE COUNT          293 a          310 c          412 g          255 t
ORIGIN      
        1 acaaacaggt caccaatgaa ccccgacgaa ctatttcgga tcgtggatgc gatccaccgg
       61 gaaaaaaaca tcgaaaaaga ggtcgtcttt aagagcattg aggcggcctt agctaccgtt
      121 gcgcgcaagc gttacggcga tcaggccgat atctcaatct cgattgatag gaaaagtggg
      181 gttatcagcg ggcgatatgg ggatcaggcg atcgatccag agcaactctc gcggatcgga
      241 gcccaggccg ccaaacaggt catccttcaa aaaatccggg aggctgagcg ggacgtcctc
      301 tacgaagaat accaagagtt actggatcag gtcgtcacgg ggacggttca gaaggtggaa
      361 ccccgggcaa tcattgtcag tttgccccgg acggaggcca tcctcccgaa gagtgaacaa
      421 atcccgggcg aaacacgcca tcccaacgag cgaatccgcg cggtggtgac cgaggtcaag
      481 aaagccggta cgcggttgcg gattgttttg agccgcattc gccctgtttt tgtggtccgg
      541 ctttttgagc aagaggttcc tgaaatcgcc gacggcgtta tcgaaattaa atccattgcc
      601 cgagagccag gttaccggac gaaagtcgcc gtggccagcg tggatcaacg ggtggattgc
      661 gtcggggcgt gcgtcggttt gcgaggtaat cgcatccgca atatcgtcga ggaactgtcg
      721 ggcgaacgga tcgatatcat ccgctgggat gatgatttga tgaccctcat ccctaatgcc
      781 ttgcagccgg ccaaggtgga tgaggtgatc ctctgccaga tgttgggccg ggcgattgtc
      841 cttgtgccgg aagatcaact ttccttggcc attggccggt gggggcagaa tgtgcgactg
      901 gcgagcaagc tgtgcgggtg ggatattgaa atcatgaccc gcgatgagct ggacgagcaa
      961 ttggaacagg ccgtccttgg gttttcctcc ttggagggtg ttacccccga gctggccgat
     1021 cgattagtcg gcgagggttt tctgtcctac gatgatctgt cggtgattga gcctgaagac
     1081 ctgatggaga tgggtggttt gaccgaggag caggttcaac acatcatcgc ccaggcagaa
     1141 gcgaaggctg aggaagccga acaattggcc agggaagaga agcgacggca gcgacaattg
     1201 gagcgggagc gtgaggccgc gctggcggaa ggtgttacaa gtgaagtgcc cccgtcagcg
     1261 gagtccggtg
//
LOCUS       DAOPTR010001146         1266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_84130_length_1266_cov_2.522709, whole genome shotgun sequence.
ACCESSION   DAOPTR010001146 DAOPTR010000000
VERSION     DAOPTR010001146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84130_length_1266_cov_2.522709"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            241..>1266
                     /gene="hflX"
                     /locus_tag="PKI05_16395"
     CDS             241..>1266
                     /gene="hflX"
                     /locus_tag="PKI05_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009094076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOA53834.1"
                     /translation="MRPRNGSQPLGEKPERGVLVGVQLPTSVLEDPPLAELAGLAKAA
                     GVEVVGQVTQRRMAPDAATYIGSGKLEDLKKLVQSQKADVVIFDNDLSPAQGRNIEKV
                     VRARVIDRTQLILDIFARRAQTYEARLAVELAQLEYTLPRLKRMWTHLERQTKGGVGL
                     RGPGEKQIEMDRRLISHRIHQLRSELEVVHQRRRREVIARKEFRTVSLVGYTNAGKSS
                     LMNALTDAGVYVEDALFATLDTRTRRWEIPGWGPVLLSDTVGFIRDLPHHLIASFKAT
                     LEEARQADLLIHVADGSNPAVFEQISSAYKVLAEIGIESKDTILVINKIDRIDDPSRL
                     HCILDRYP"
BASE COUNT          301 a          326 c          358 g          281 t
ORIGIN      
        1 ttactgtcat ttggtactgg atgaagaggt tgaggaggaa gaagagtaat cacgcaggag
       61 agtagtgttc aatgtcgtgt tccgcttgga gtttttgacg gctagaacaa aactgtcaca
      121 gccctatgcg tgggcagaac atcgaaatcg cttcgagatc acacggatat ttgggaggac
      181 gtgtaccccg gaaaggtgtt cgtaacaacc gtaaatagca atcggaggtg tgacgacatc
      241 gtgaggccca gaaatggcag tcaaccgctt ggtgaaaaac cggagcgagg ggtcctggta
      301 ggtgtgcaac ttccgacatc cgtccttgaa gatccgccat tggcggagct agccggcttg
      361 gcaaaggccg ccggggtgga ggtcgtgggt caagttacgc agcggcggat ggcccctgat
      421 gccgccactt acattggcag cggaaagctc gaagacctga agaagctagt ccaatcgcag
      481 aaagcggacg ttgtcatttt cgacaatgat ttgtctcccg ctcaaggccg taacatcgaa
      541 aaggtggtac gggctcgcgt gatcgatcgg acccaattaa ttctcgatat tttcgcccga
      601 cgtgctcaaa cgtacgaggc ccgattggcc gttgagcttg cccaattgga atacactctg
      661 cctcgactca agcgaatgtg gacacacctt gaacgccaga caaaaggtgg cgtgggcctg
      721 cgcggtcctg gtgaaaaaca gattgaaatg gaccgccgcc tcatttctca tcgcatccat
      781 cagttgcggt ccgagctgga ggttgttcat cagcggcgtc ggcgagaagt catcgcgcga
      841 aaggagtttc ggactgtttc ccttgttgga tacacaaacg ccggcaaaag cagtttgatg
      901 aatgccttga ccgacgcggg cgtgtatgtt gaggatgcct tgttcgccac gttagatacc
      961 cgcacgcgcc gctgggaaat ccccggttgg ggccccgtac ttttgagcga taccgttgga
     1021 tttattcgcg atctacccca tcacctcatc gctagcttca aagccacact ggaagaagca
     1081 cgtcaggctg atctgcttat ccacgtggcc gatggcagta acccggccgt attcgagcag
     1141 attagctccg cttacaaggt ccttgccgag atcggaatcg aatcgaagga cactattctc
     1201 gtcattaaca agatcgaccg gattgatgat ccgtcacgtc tccactgcat cctagatcgc
     1261 tacccc
//
LOCUS       DAOPTR010001147         1265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_84199_length_1265_cov_3.570248, whole genome shotgun sequence.
ACCESSION   DAOPTR010001147 DAOPTR010000000
VERSION     DAOPTR010001147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84199_length_1265_cov_3.570248"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..1068
                     /locus_tag="PKI05_16400"
     CDS             37..1068
                     /locus_tag="PKI05_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53835.1"
                     /translation="MSYRWLLLASLVAGFLLTVTGTGQVLGEEPSRVLPEGPGLAAQC
                     GDTEVLAKHPEVVFMEDFEVGQFSDLKGRWTQISHPEIMTLSDLVPPDSHGKRSLLMT
                     HTGGKGTGGHLYRRLNQGYEKLFIRFYVRFAPDCGEIHHFFHVGGYNPPTPWPQGGAG
                     ERPRGNERFTVGIEPFGRDWRWDYYTYWMEMRGSPPRGQTWGNCFINDPKLRVKKGEW
                     QCLEVMIKLNSPVTESNGELALWVDGKLVSHLGPGFPKGKWTYDRFLPGQGGRAIRWN
                     DEKGGPETFSVPEGGEPFPGFRWRNDEDLKINFVWVLLYITKTPPGQVSQVWFDDIVV
                     AKSYIGPNK"
BASE COUNT          292 a          329 c          370 g          274 t
ORIGIN      
        1 gaatgttttc ggcaatctta gtgggaggac gtaacgatga gttatcgctg gcttttgttg
       61 gcatctttgg tcgctggatt cctgctgacg gttactggca cgggtcaggt cttaggggag
      121 gaaccttctc gcgttttgcc cgagggtccc ggtttggccg cccaatgcgg agacacggag
      181 gtccttgcca agcacccgga agtcgtcttc atggaagatt ttgaggtagg gcaattcagc
      241 gatctaaaag ggcgatggac gcaaatttcg catccggaga ttatgacact ctccgacctc
      301 gtcccgcccg acagccacgg taagcggtcg ctgttgatga cgcacacggg tggtaaaggg
      361 accggaggtc atctctaccg ccgactgaat caaggctacg agaagttgtt tattaggttc
      421 tacgtgcgat tcgcccctga ctgtggcgag attcatcact ttttccacgt gggcggatac
      481 aaccctccga ccccgtggcc acaaggcgga gctggcgagc ggcctcgcgg aaacgagcgg
      541 tttactgtcg gtatcgaacc gttcggacgc gactggcggt gggattatta cacctactgg
      601 atggaaatgc gcgggagtcc accgcgcggg caaacctggg gaaactgttt catcaatgat
      661 cccaaacttc gggtcaaaaa aggcgaatgg caatgcctgg aagtcatgat caaactcaat
      721 tccccggtca cggagagtaa cggcgagttg gccctctggg tggatggaaa actggttagt
      781 catctcggtc ccgggttccc taaagggaaa tggacttacg atcgcttcct ccccggtcag
      841 ggtggtcggg caattcgctg gaatgacgaa aaaggtggtc cagaaacctt ttccgtaccc
      901 gaggggggag aaccctttcc tggctttcgc tggcgaaatg acgaagatct gaaaatcaac
      961 ttcgtctggg tcctcttgta tatcaccaaa accccgccag gccaagtaag ccaagtctgg
     1021 ttcgacgata tcgttgtggc caagagctac atcgggccga ataaatagtc gcaagcaaca
     1081 ataggccttt ctcgccaaga taataagaac ctatccgaaa aagtccccct ctccctccgg
     1141 gagagggttg gggtgagggc acgcgaccgt cggaattgat tgagaaaacc ggctgcgatt
     1201 cgagccagcc tcacccccgc cccctcccgc cgaaagggta aggggagatt atgggataag
     1261 ctcta
//
LOCUS       DAOPTR010001148         1265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_84214_length_1265_cov_3.306612, whole genome shotgun sequence.
ACCESSION   DAOPTR010001148 DAOPTR010000000
VERSION     DAOPTR010001148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84214_length_1265_cov_3.306612"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="PKI05_16405"
     CDS             complement(<1..783)
                     /locus_tag="PKI05_16405"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOA53836.1"
                     /translation="MDSLKVAIIGFGTIGSGVAKLLLEHGDRIARRAGRPIELVRIVD
                     KDLVRPRNVTVPKHLLTDDLDAVIKDPNIAVAVELIGGVEPTRSIVLSLLEHGKDVVT
                     ANKALLAEHGPELFEAARRLGRSISFEAAVAGGIPIIAAIGESLTANEITSIHAILNG
                     TSNFILTQMEERGQEYAAALAEAQRLGYAEANPSMDVSGADAAQKLAILAQLAFGAQI
                     NWRDIPRVGIDTVQAEDMRYARELGYHIRLLAVAELIDGELEL"
     gene            1065..>1265
                     /locus_tag="PKI05_16410"
     CDS             1065..>1265
                     /locus_tag="PKI05_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008669709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53837.1"
                     /translation="MKTLVTMMCRDVYLTWVSWLLMLTLLASLGLVEASQGSGAERPN
                     VVLVITDDQGYGDLACHGNPVIK"
BASE COUNT          269 a          350 c          355 g          291 t
ORIGIN      
        1 caactcaagc tcaccgtcga ttagttcagc tacggccaac aggcgaatgt gatacccaag
       61 ctctcgagca taccgcatgt cctccgcctg gacggtgtcg atccccaccc gcgggatatc
      121 cctccaatta atttgggcac cgaaggcgag ctgagcgaga attgcgagtt tttgggccgc
      181 gtcggcaccg cttacatcca tggaaggatt tgcctcggcg tagcccagtc gctgggcctc
      241 cgccaaagcg gcggcgtact cctgaccacg ttcctccatc tgagtcagga taaagttact
      301 ggtaccatta aggatggcgt ggatcgaggt aatttcgttg gcggtcaggc tttcgccgat
      361 agccgcaatg atcggaatcc cgcccgctac cgcggcctcg aaggatatcg aacgccccaa
      421 acgtcgtgca gcctcaaata gctcggggcc gtgctccgcc aatagggcct tgttggccgt
      481 aaccacgtct ttgccgtgct ccaaaagcga caggacaatg gaacgcgttg gttccacgcc
      541 gccgatcagt tcgaccgcca cggcaatgtt ggggtctttg attacggcgt ccaaatcgtc
      601 ggtcaaaaga tgctttggca ccgtgacgtt gcggggccgg acaaggtcct tgtccacaat
      661 ccggacaagc tcgatcggcc gccccgcccg ccgagcaatc cggtcaccgt gttccaatag
      721 caattttgcg acaccagagc cgatggttcc gaagccgata atagcgactt taaggctgtc
      781 catattgggt gcctattccc atgattgcca cgaaaggcca cttttccgat aatcatcttc
      841 gacaaaatgg aacgccttgc caggcatggg cggcctgttc tgctaaaagc tgtaaaccgt
      901 tatcctaggt tgacgctcga aaagccgtca agatgttccc ggccgcggaa gggcctttgc
      961 cgcttggttg gttgtacgcc atcctttaag ccagcgcaag aatgaaacga cttttggaac
     1021 agatgctctc ttagatcaga tttccgttta cggggaggtc tcagatgaaa acattggtga
     1081 ctatgatgtg ccgggacgtg tacctgacct gggtttcgtg gttgttaatg ctgaccttat
     1141 tggcttcgct gggacttgtt gaggcaagcc aggggagtgg tgcggaacgc cccaatgtcg
     1201 tgttggtgat tacggatgat cagggctatg gcgatttggc ctgccatggt aatccggtca
     1261 tcaag
//
LOCUS       DAOPTR010001149         1265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_84221_length_1265_cov_3.052893, whole genome shotgun sequence.
ACCESSION   DAOPTR010001149 DAOPTR010000000
VERSION     DAOPTR010001149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84221_length_1265_cov_3.052893"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(285..>1265)
                     /locus_tag="PKI05_16415"
     CDS             complement(285..>1265)
                     /locus_tag="PKI05_16415"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III-family protein"
                     /protein_id="HOA53838.1"
                     /translation="RDALAAARKTYPAFLDQYIEKMATFLGKILHPDGQIPLFGDSTL
                     DLTPQPRQVFTRLQMGFPSLCPPQPQSYAVGDYWIYREQENFLIFDGGPVGPDYLPAH
                     AHADLLTVEASWAGTRLFVDGGVYDYEDSPERAYCRSTAAHNTAEINGQNQCDVWSRF
                     RMGRRGWPGKLQTGLTGRFHWARCTHNAYRHLNVPSVGRWIVCTEGGPWCIVDSCKGS
                     PSVTMASRLRLAPGWIPSHETPNRLTLERGSVRLLVENILGCGPIKCDIGNYYPEFQK
                     AVQIPVLYYETTNRGSCQLAWLVSGEPFGPFVFKPLSREGALAGNSVSKS"
BASE COUNT          286 a          359 c          333 g          287 t
ORIGIN      
        1 cgaacaggta cccgccggtt atggctattg ttctcgagat ggaaacgagg tgtttctcgt
       61 ttcgctgagg tccatcgaaa acaggaaatc caacgcgcgg tatgatctaa cgcgttggtt
      121 gttcttagaa tctcccctcg cccgttcggc gggagagggg tcgggggtga aggcggctcg
      181 aatcgctgcc ggttttctcg atcgatttcg acattcgctt accctcaccc catccctcgc
      241 ccgaagggag agggacactt tttgcgatag actgtaagtc cgcatcaaga tttgcttacg
      301 ctattccctg cgagagcccc ctcccggctg agaggtttaa acacaaaggg cccgaaaggc
      361 tcgccagaaa ctaaccacgc aagttggcat gaacccctgt tggtcgtctc atagtacaaa
      421 acgggaatct ggaccgcttt ctgaaactcg ggataatagt ttccgatgtc acatttgatg
      481 ggcccgcaac ccagaatgtt ttcaaccaac aagcgaaccg atccgcgctc caaagtaagt
      541 ctattcggtg tctcatgcga tggaatccat cctggtgcca atcgtagtcg gctggccatt
      601 gtgactgaag gtgatccttt acacgaatca acgatgcacc agggtccccc ttcagtgcag
      661 acaatccatc ggccgacgct cggtacgttc aagtgtcggt aggcattatg agtgcaccgc
      721 gcccagtgaa atcttcccgt tagccctgtc tgcagtttcc ctggccagcc ccggcgcccc
      781 attcgaaacc gcgaccacac atcacactgg ttctgcccat tgatttccgc agtattgtgc
      841 gccgccgtgc tgcggcagta ggctctttcc ggagaatcct cgtagtcata aaccccgccg
      901 tccacgaata gccttgttcc tgcccaggag gcctccactg tcagcaagtc ggcgtgggcg
      961 tgagcgggca agtaatccgg gcccaccggc cccccgtcga agatcaaaaa attttcttgt
     1021 tcacgataga tccaatagtc accaacagca taggactgtg gctgtggcgg gcaaagacta
     1081 ggaaagccca tttgcagtcg ggtaaacacc tgtctcggtt gtggagtcaa gtcaagggtg
     1141 ctatcaccga aaagaggaat ctggccatcg gggtgcaaga tctttcctag gaaagttgcc
     1201 atcttttcaa tatactggtc gagaaaggcc gggtacgtct ttcgagccgc agccagggca
     1261 tcacg
//
LOCUS       DAOPTR010001150         1264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_84335_length_1264_cov_2.337469, whole genome shotgun sequence.
ACCESSION   DAOPTR010001150 DAOPTR010000000
VERSION     DAOPTR010001150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84335_length_1264_cov_2.337469"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            566..>1264
                     /locus_tag="PKI05_16420"
     CDS             566..>1264
                     /locus_tag="PKI05_16420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53839.1"
                     /translation="MFRQLWAIDRSNPRYCSGNGESIKALTSRLVFSLVIVGLIGVIN
                     LACGGARLLAEETNPTASQTESPEKGDERFGRVIPVRLPINGPTSARVKQTIQKILTE
                     FKGSEGQLVLILRFDTNRGEEQFAARSEFGACYDLADFLTSEALSKVHTVAYVPYRAE
                     GHAVLPIMACRDIILAPDAEWGAAGAAERRITPPMVSAYRHIAERSRTIPPAVVLGML
                     DASRAVLLVETDVER"
BASE COUNT          273 a          343 c          356 g          292 t
ORIGIN      
        1 gccgacgaca tcgtcgcggt gcaagttcag tattttggac tacctaaggg tgcgcaaagc
       61 catctgacaa ttgtttgttt gtctcacatt tctttgctcc tcgtcttgcg acagggactg
      121 atgtcgttaa attcccgtac tcgtgcttaa cccactgtcg tgggaagcca cgcgctgtcg
      181 ggatcctgcc aaatgagtgg tgcgccagtc cgtacatgcc ggggatattg cctcgccaat
      241 gcatgctggc gtctcacgcc acgccgacca tgacagcgcg gagcagtgca gattgaacgc
      301 ggactgctgc gcctttccat ctcgaacgtg gtttccttgg ccaagccgtt tcccaggtat
      361 gggagttagc tcgaactatt tcaaaaggct gttacgctgg tgctgggacc cgatacaatg
      421 aacgagaggc ggcggcaacc tataaggcaa ctcgcgattt gctgctgcgt taccgttaag
      481 aatcgtccga gatagcaaga tgcaacctta gacccggggc tggtcaagtc ggtcggacgt
      541 tgcaacagac aaaaggagcc tgttcatgtt caggcaactc tgggcaatag atcgttcaaa
      601 tccccgttac tgttccggta atggagaatc cataaaagca ctcacatcac gcctcgtctt
      661 ttcgctcgtc atcgtcggct tgataggcgt gattaacctg gcctgcggtg gggcacggtt
      721 actagcagaa gaaaccaatc ctacggcgtc tcagactgaa tcgccagaaa aaggcgacga
      781 gagatttggg cgggtaatcc cggttcggct tcccatcaac ggtccaacaa gtgcccgcgt
      841 taagcaaacg atccagaaga tccttaccga attcaaaggg agcgagggcc agctcgttct
      901 cattctccgt ttcgatacca atcgcggtga ggagcaattt gcggcaagat ctgagtttgg
      961 tgcctgctac gatttggcag attttttgac gagcgaggca ctctctaaag ttcacacagt
     1021 tgcgtatgtc ccctatcgcg cggaggggca cgccgttttg ccaatcatgg cgtgccgcga
     1081 tattattctt gctcccgacg cggagtgggg ggcagccggg gcggcggagc gacgcatcac
     1141 ccctcccatg gtgagtgcct accgccatat tgccgaacga agccgtacga ttcctccggc
     1201 ggtggtgctt ggtatgctcg atgcctcgcg agcagtcctc ttggtggaaa ccgacgtgga
     1261 acga
//
LOCUS       DAOPTR010001151         1261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_84595_length_1261_cov_3.037313, whole genome shotgun sequence.
ACCESSION   DAOPTR010001151 DAOPTR010000000
VERSION     DAOPTR010001151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84595_length_1261_cov_3.037313"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PKI05_16425"
     CDS             <1..311
                     /locus_tag="PKI05_16425"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOA53840.1"
                     /translation="AIRPRVWRSLQAGKSLYLTFAEAEIFPQDWLDVLRVGEESGKLD
                     EALSHLSRVYADRAAQAMKILSVVGAVAVTLLIAGVIIFLIFRLALFYIGQIQSALPA
                     "
     gene            complement(342..911)
                     /locus_tag="PKI05_16430"
     CDS             complement(342..911)
                     /locus_tag="PKI05_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOA53841.1"
                     /translation="MPVSVSLGGCVVELVQGDITEQQVDAIVNAANSRLAGGGGVDGA
                     IHRRGGPEIMAETRAKYPEGCPTGSAVISGAGRLPAKYVIHAVGPIYRDGQHGEAELL
                     ASAYRKSLSLAIEHGCQSVAFPALSCGAYGYPVEEAAKIALGTTMEFLRQHGKPALVR
                     FVLFGQSTYDTFLKALKDLVGQDAVTERT"
BASE COUNT          293 a          376 c          314 g          278 t
ORIGIN      
        1 cggcaatccg gcctcgtgtc tggcgttcgc ttcaggctgg gaagtcgctc tatcttacct
       61 ttgccgaagc agaaatattt ccccaagatt ggttggacgt gctccgcgtc ggtgaagaga
      121 gcggcaaact ggatgaagct ctcagtcacc tcagccgtgt ttatgccgac cgcgcagccc
      181 aggccatgaa aatactcagc gtcgttgggg ccgtggccgt gacgttattg atcgcaggcg
      241 ttattatctt cctaatcttt cgccttgcgc ttttttacat tgggcagatt cagagtgcat
      301 tgccagcttg aagaacccca gcacaccttg acatatcaca ttcaggtccg ttccgtgaca
      361 gcgtcttggc caacgaggtc tttcagcgcc ttgagaaacg tgtcgtatgt gctttgacca
      421 aacaggacaa atcgaaccaa ggccggtttt ccatgctgcc gcaagaactc catcgtggtt
      481 ccgagggcaa tcttggctgc ctcttcaacg ggataaccgt aggccccaca actaagggca
      541 ggaaaggcaa cgctctggca gccatgttcg atggctagac taagcgattt gcgataggcc
      601 gaggcaagca attccgcttc gccgtgttgg ccatcgcggt aaatcggccc cacagcgtga
      661 ataacatact ttgccggcag ccgacccgcg ccagatatta cggcagaacc cgtggggcaa
      721 ccctccggat atttagcacg tgtttcggcc atgatctcgg gtcctccacg gcgatgaatg
      781 gcaccatcga cacctccgcc accggccaaa cggctgttgg cggcattgac aattgcatca
      841 acctgctgct ctgtgatgtc cccttgaaca agctcgacca cacatccacc aagggagacg
      901 cttaccggca tatctctgtc tccatgcaac gattagtccg agaatccgcc ttgaaacctc
      961 actatcgacg gaaagcaaat tcccccgaaa gatttgactg gcaacgccca caaaaacgca
     1021 acccgtgtgg catcaactaa accggcctaa aaagcctttt tcctggcaca accctattgt
     1081 gcggacgcgc gaaagtcttt tcaacgcctc gccctgcaag tctcctttgg aaatccgaca
     1141 acaataaaag gggatcgtcg ccgggcccca atatcgctca acgatcgcgc cattggtatg
     1201 ataaatagca ttatgggatc ggcttgctcc gatcgagtga gccggcccct cccgaagtcc
     1261 g
//
LOCUS       DAOPTR010001152         1257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_84965_length_1257_cov_2.891847, whole genome shotgun sequence.
ACCESSION   DAOPTR010001152 DAOPTR010000000
VERSION     DAOPTR010001152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84965_length_1257_cov_2.891847"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PKI05_16435"
     CDS             complement(<1..657)
                     /locus_tag="PKI05_16435"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOA53842.1"
                     /translation="MVILGISDLHGRIDALAAILDQVDRCDIVALAGDITHFGSPKDV
                     DQIIELCRSHADHVFAVAGNCDSASIDQRLNELGVGLAGRGVVINGVGIHGLSGIPPW
                     RPGMYQFPEDELASYLQVGFQQIAGSSVHLLLTHVPPRQCGVDRVIWGCHVGSRAVQE
                     FLSTAQPDLVLCGHVHEGRGIERQGKTMIVNCGHAASGYYAKIVIPSRDSENQIQVEL
                     C"
     gene            932..>1257
                     /locus_tag="PKI05_16440"
     CDS             932..>1257
                     /locus_tag="PKI05_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53843.1"
                     /translation="MKITAIDLYLLSLPLRTPIRFETVPFSTDPCEWSRLETVLVRLW
                     SGGAYGWGEACPGNGPFWSAEWAGAIFYCLRDWLAPRLIGREYATPEELHERVAVFRG
                     WRAAQAA"
BASE COUNT          291 a          328 c          362 g          276 t
ORIGIN      
        1 gcaaagctcg acttggattt gattttccga gtcgcgggaa gggatcacaa ttttcgcgta
       61 ataaccagag gcggcatgac cgcagttgac gatcatcgtc ttgccttgtc gttcaatacc
      121 acgtccctca tggacatgac cacagaggac gagatccggc tgtgccgttg aaagaaactc
      181 ctggacggca cgacttccca catggcaacc ccagatgacc cggtccacac cgcattgcct
      241 tggaggtaca tgggtgagga ggagatgaac cgagctccct gcgatctgct gaaacccgac
      301 ttggagatat gaagccagct cgtcttcggg gaattgatac atccccgggc gccatggtgg
      361 aatgccggac agaccgtgga ttcccacccc attgatcacg acaccacgtc ctgctagccc
      421 cactcctaat tcgttgagcc gctggtcgat ggaagcacta tcgcaattgc cggcaacagc
      481 gaacacgtgg tcagcatgag atcggcaaag ttctatgatc tgatccacat ccttgggtga
      541 tccaaagtga gtgatatcgc ctgctagggc gacgatgtca caccgatcaa cctgatccag
      601 tattgctgcc agtgcatcga ttcttccgtg caggtcagaa atgccgagga ttaccatcgc
      661 gcaagataaa atcggtggca gaaggtgtgt gacagtcaag aatcgctgaa gaacaatggc
      721 cgtatcatgg ttatcatagg gcaatcacgg ccagcgacaa gttaggacgg atgaaccagg
      781 gggcttatct tgttacaaaa cgtgtcttca ggcgttggtt cgtgaggccc aggaaacacc
      841 aatcccgctt gaaaaagacg cggagacgtg agaccattga gcaagaaggg ttgcaaacca
      901 ctttgggttc tgaggcagaa gcggcggatt catgaagatc acagccattg acctttatct
      961 tctttctttg ccattacgga cgcccattcg gttcgagacg gttcccttca gtaccgaccc
     1021 ttgtgaatgg tctcggctgg agacggtctt agtccggctg tggagtggag gagcctatgg
     1081 gtggggcgag gcgtgtcccg gcaacggtcc cttttggtcg gcggaatggg cgggtgccat
     1141 attctattgc ttgcgggatt ggcttgctcc tcgccttatc ggacgtgaat acgcaacccc
     1201 ggaagaactt cacgagcgag tagccgtgtt ccgtgggtgg cgagcggccc aggctgc
//
LOCUS       DAOPTR010001153         1256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_85044_length_1256_cov_4.239800, whole genome shotgun sequence.
ACCESSION   DAOPTR010001153 DAOPTR010000000
VERSION     DAOPTR010001153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85044_length_1256_cov_4.239800"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            467..>1256
                     /locus_tag="PKI05_16445"
     CDS             467..>1256
                     /locus_tag="PKI05_16445"
                     /inference="COORDINATES: protein motif:HMM:NF024927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HOA53844.1"
                     /translation="MCGIVGCVGYSNALEFLLAGLRRLEYRGYDSAGIALAHMERAEA
                     IHVRKTAGRIDRLVSLIEAHPISGNIGIAHTRWATHGGATDRNAHPHLGGDGEVAVVH
                     NGVIENCDSLRTRLEGLGYTFRSETDTEVIAHLIAQLLREAHLDPQEVVAKGYTPLVQ
                     VIQSALSQLRGTWGLAILFRDYPGIIVGARLGSPLIVGTGNGANFLASDASALVGVAD
                     NVVFLSDNEIALITGEWVRVYHRDQGRVQPDFRRLDMSASDVDTG"
BASE COUNT          254 a          333 c          387 g          282 t
ORIGIN      
        1 tatgggacag gttcttaaga gacaatgggc cacttgtgtg ctcttcgccg gacgtcatcg
       61 tagtttgctg gttttagttt gacgcccgag gtcaagtccg actaaagtgg tatagatggc
      121 acaccgtttg cgcgagcgtc cgtgatcgcc gggggagatt tctccagatc gcctatcttg
      181 tcatatcagc cgggaaaagt ccggaaactc ctcaaaatca accgtgaacg ttttggcgaa
      241 aagtgttatt ctcggcttag agtctatccc aaaaagctcc ctctccctcc gggagagggt
      301 tggggtgagg gcaagcgatc accggagtcg attgagaaaa ccggtagcaa ccagagccac
      361 cctcgccccc ggaccctctc ccgccgaacg ggtgagagga gattatggga gaggctctta
      421 gccgtttgct cgtgtcacaa atgtcgcgta aggagtatcg agatccatgt gtggaatcgt
      481 gggctgcgtt ggctactcga atgctcttga gtttcttttg gcagggctga gacggctgga
      541 ataccgtgga tacgactcgg ccggtatcgc tttggctcat atggagcgcg ctgaggcaat
      601 tcacgtccgc aagaccgccg gacggattga tcgcctggtg tcgctcatcg aagcgcatcc
      661 catttccgga aatatcggaa ttgcccatac ccgctgggca acacacggtg gggccaccga
      721 tcgcaatgcg catccgcact tggggggcga cggcgaagtg gctgtagtcc ataacggcgt
      781 cattgagaac tgcgattcct tgcgaacccg tttggaaggc ttgggctaca cctttcgatc
      841 ggaaacggac acggaagtca ttgcccatct gatcgcccaa ctcttgcggg aggcacatct
      901 ggaccctcag gaggtcgtcg ccaaggggta cacgccgctc gttcaagtca ttcaatcggc
      961 cttgtcccaa ctgcggggga cctgggggtt ggccattctg ttcagggatt atcccggcat
     1021 tatcgtcggt gcccgactgg gcagtccttt gatcgtcggg acgggaaatg gtgcgaattt
     1081 cctggccagc gatgcctcgg cgttggtcgg ggttgcggac aacgtcgttt tcctcagtga
     1141 caacgagatt gccttgatca cgggcgaatg ggtgcgggtc tatcaccgag atcaggggcg
     1201 agtccagccc gacttccgca gactcgatat gtcggcgagc gacgtcgata cggggg
//
LOCUS       DAOPTR010001154         1251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_85497_length_1251_cov_3.964047, whole genome shotgun sequence.
ACCESSION   DAOPTR010001154 DAOPTR010000000
VERSION     DAOPTR010001154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85497_length_1251_cov_3.964047"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PKI05_16450"
     CDS             complement(<1..401)
                     /locus_tag="PKI05_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HOA53845.1"
                     /translation="MVRLISRSVFRENDAAICRRDRASIARCRLLGAVMILGGAILLS
                     PLEALCVEQAPFAMRRPVPDLPENLEWLNTTGPLTWRDLRGKFVLLDFWTYCCINCMH
                     IIPELKKLEAKYPRELVVVGVHSAKFQEEKES"
     gene            complement(838..>1251)
                     /locus_tag="PKI05_16455"
     CDS             complement(838..>1251)
                     /locus_tag="PKI05_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53846.1"
                     /translation="ILRNGLVLVIGREFGWKPLTAGTLVDLAAWLVAGALVLGLLRGE
                     YRRFLDCLRREVEEGWLKPEQAELLKRQFWRARLRMIFPLWQDRTCRVIFLFSELAQQ
                     QVVAAETARGVGGHNISQRLHQELVKLVSQLPQAS"
BASE COUNT          289 a          375 c          353 g          234 t
ORIGIN      
        1 gattcttttt cctcctggaa tttggcggaa tggaccccta cgacgactaa ctctcgcgga
       61 tatttggctt cgagtttctt caactcggga atgatatgca tgcagttgat acagcagtag
      121 gtccagaagt cgaggagcac gaatttcccg cgcaaatccc gccaggtcaa cggcccggtg
      181 gtgttaagcc actccaggtt ctcgggcaag tctgggacag gacgacgcat agcaaaaggg
      241 gcttgttcca cgcaaagggc ctccaggggg gaaaggagta tcgcaccgcc aagaatcatc
      301 actgcaccca aaagacggca tctcgcaatg gacgcccgat cccgacgaca gattgcggcg
      361 tcgttctcgc ggaataccga tcgacttatc aagcgaacca tagacgttac cctttttcgg
      421 ctatcagcgc ggtggactgg gacgccaatc acttgaagcg gccacgaaca agcgtagccc
      481 tcaatcggcg aaggtcacca cgacggcgcg tggtattccc tatcaatgtc tcgaggcaca
      541 cctgaccgtt catgacaaaa ccacgctgtg atgaatcgta ttcgccaggg accctttgtg
      601 gcaaggacgc tgtggcgatt gggaaacaca ccgaatacac cgcgttaccc agagcctatc
      661 ccacaagttc ccctctcccg ttcggcgaga aagggatgag agtggctcga atcgctgccg
      721 cttttctcga tggactccga cgttcgcttg ccctcacccc aaccctctcc cagaaggaga
      781 gggaactttg ggataggatc ttaagaggat aaaggccagc catcaaaatc cggcgaatta
      841 ggaggcctgg ggtaactgcg agacaagctt gaccagctct tggtgaagcc gctgggaaat
      901 gttatggccg ccgaccccgc gggccgtctc cgccgcaacg acttgctgct gggccagttc
      961 gctaaacagg aagatgacgc gacaggtgcg gtcctgccaa agcgggaaga tcatccgaag
     1021 gcgtgccctc cagaattgtc gcttgagcaa ttcggcctgc tctggtttga gccagccctc
     1081 ttcaacttcc cgccgcaggc agtcgaggaa tcgtcggtac tccccgcgga ggagccccag
     1141 gaccaaggcc ccggccacga gccaggccgc gagatcgacg agcgtgcccg ccgtgagcgg
     1201 cttccaaccg aactcccgcc cgatgaccag gacaagtcca ttgcggagga t
//
LOCUS       DAOPTR010001155         1250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_85609_length_1250_cov_3.405021, whole genome shotgun sequence.
ACCESSION   DAOPTR010001155 DAOPTR010000000
VERSION     DAOPTR010001155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85609_length_1250_cov_3.405021"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..>1250
                     /locus_tag="PKI05_16460"
     CDS             36..>1250
                     /locus_tag="PKI05_16460"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOA53847.1"
                     /translation="METGRLYDVDGNGTLDVLPNGMKFAAWWEFRREDTGNVRWIRHD
                     LPREIAGHGIGFGDVNGDGRGDVVTPIGWWEAPKDRANGEWIAHREWVLHRDASVPII
                     VEDIDGDGDSDIMWGRGHDVGLYWLEQARDGDSRRWTFHVIDTSFSQIHSILVTDLDN
                     DGRREFVAGKRYLGHEGRDPGEWNPLAIYWYRFDPGRRTFIRGVVDEGGPVGFGLDPK
                     AADIDGDGDIDLVASDRSGLYLLENLLKSDPEQTSDVLPPSSEEGPWTKEDHRNVLLY
                     VDENNETKTVSSLKEWGIRRAHILKGMMAAMGPLPGPEHRVPLDIEIVSEEMTESYRR
                     LKLTYAADESDRVPAYLLIPTRPSQRKPAVLCLHQTTPLAKDEPAGTHPDAAWPYAHE
                     LALRGFVCIVPDY"
BASE COUNT          272 a          315 c          388 g          275 t
ORIGIN      
        1 cttggcaggt tcatctcatc gagaaacccg gtcccatgga aacgggccgt ctttacgatg
       61 tggatggaaa cggcacgctg gatgttttgc ccaacgggat gaaattcgcg gcgtggtggg
      121 aatttcgacg ggaggatacg ggaaatgtgc ggtggatccg tcatgatctg ccccgtgaaa
      181 tcgccggtca cggaataggc tttggcgatg tcaacggcga tgggcggggc gatgttgtaa
      241 ctcccatcgg atggtgggag gcaccgaaag atcgtgccaa cggtgagtgg atcgcgcatc
      301 gtgaatgggt actgcaccgc gatgcttcgg taccaattat cgttgaagac atcgatggcg
      361 atggtgatag cgacatcatg tggggacgcg gccacgatgt ggggctctat tggcttgaac
      421 aggcaagaga cggcgactcc cgtcgttgga cgttccacgt gattgataca agtttttcgc
      481 aaattcactc tattctcgtg acggacctgg ataacgacgg ccgtcgtgaa ttcgtggccg
      541 gcaagcggta cctaggccac gaagggcgtg atcccggtga gtggaacccg ctggccattt
      601 actggtatcg cttcgatccg ggacgaagaa catttatcag gggcgttgtc gatgagggag
      661 gaccagtggg gtttggtctc gaccctaaag cggcagacat tgacggcgac ggtgatatcg
      721 atctggtggc ttctgatcgc agtggcctgt atctgctaga gaatctgtta aaaagtgacc
      781 cagagcaaac gagtgacgtc ctgcctccgt cctcagagga aggcccctgg accaaggagg
      841 atcatcgcaa tgtgcttctc tatgtagatg agaataacga gacgaaaacg gtttcgtcct
      901 tgaaggagtg gggcattcgc cgcgcccaca tcctcaaagg gatgatggcc gcgatgggcc
      961 cactccccgg tccggagcat cgcgtgccgc tcgatatcga aatcgtctcc gaagagatga
     1021 cggaatccta tcgtcgcctg aaactgacgt atgcagcgga cgagtcggac cgggttcctg
     1081 cttatttgct cattccaacg aggccctcgc aacgcaagcc cgctgttttg tgtttgcacc
     1141 agacgacgcc attggccaag gacgaaccgg caggtacgca tcccgatgct gcttggcctt
     1201 atgcccatga actcgcccta agaggttttg tgtgcatcgt gcctgactat
//
LOCUS       DAOPTR010001156         1250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_85610_length_1250_cov_3.402510, whole genome shotgun sequence.
ACCESSION   DAOPTR010001156 DAOPTR010000000
VERSION     DAOPTR010001156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85610_length_1250_cov_3.402510"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="PKI05_16465"
     CDS             complement(<1..1017)
                     /locus_tag="PKI05_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53848.1"
                     /translation="MAQSVVVSLKEKINNQWRDRPRSQGSLLSAYGEPAIWLTGGALV
                     ICLAMILGLLLLILINGLRTFWPARVVHIKLRNGQAVLGEITRSEWYRPDPAFLEQLS
                     PDQRALYVEAIEAHDGWVKRELIRIGNYELTNEHFRWVALPEIAPGGGTFPDWALVIE
                     RVTWGRFYGFPVGFVVGETVTATEPQQIWQEFQKHHPRVRQRWRRIRQLETRTLGDIN
                     YALEKSRLRLRTIELREGKSSPAYQELAQQFARQEAELQQEYDSVRTEIESLRRENSQ
                     YGLRLRTADGQEKDIGLADIVRAYPANQLSLGERLALYGSRVWEFLSDEPREANSEGG
                     IFPAI"
BASE COUNT          286 a          362 c          318 g          284 t
ORIGIN      
        1 gatagccggg aaaattcctc cctcgctgtt agcctctcgc ggttcatcgg aaagaaactc
       61 ccagacgcga gagccgtaaa gggctagtcg ctcccccaaa ctcaactgat tggccggata
      121 cgcacgcaca atgtccgcca ggccgatgtc tttttcttgg ccatcggcgg tccgcaaccg
      181 taagccgtat tggctattct ctcggcgaag gctctcaatc tcggtccgca cggaatcgta
      241 ctcttgttgc aactccgctt cttgccgggc aaattgttgg gctagttcct gataggcagg
      301 ggagcttttc ccttcccgca gctcgatggt tcgtagtcgc agccgtgatt tttcgagggc
      361 gtaattgata tcgcccagtg tgcgggtttc caattggcgg atgcgtcgcc atcgctgacg
      421 gacccgcgga tgatgtttct gaaactcttg ccaaatttgc tggggttccg tggccgtcac
      481 agtctcgcca acaacaaacc ccacaggaaa tccgtaaaag cgaccccaag tgacgcgctc
      541 gatgactaag gcccagtcgg gaaaagttcc accgcctggt gcaatttcgg gcagcgccac
      601 ccagcgaaaa tgctcgttcg taagctcgta attcccaatc cgtatcagtt cccgtttaac
      661 ccacccgtcg tgtgcctcta tcgcctcgac gtacagcgca cgttgatctg gcgatagctg
      721 ctccaagaaa gcgggatccg ggcgatacca ttcgctgcgg gtgatttctc caagcacggc
      781 ctgaccattc cgaagtttaa tgtgcaccac ccgtgccggc caaaaggtcc tcagcccatt
      841 aatcagaatg agaagtagca ggccgagaat catcgccaga cagatcacca aggctccccc
      901 ggtcagccaa atcgctggtt ccccgtaggc agaaagcagg cttccttgcg acctagggcg
      961 atcgcgccac tggttattga tcttttcctt taaggatacg acgacactct gcgccatgat
     1021 tggctcaccg gacaatcaaa aaccgtgcat gcttaagcat actccaaatc tcgaagatca
     1081 taatcttttc agagttgata ggccttcttg cggaatttca gccttaccaa ttcggccagt
     1141 gtgttgatca cgaaggtcat gacaaaaagg caaagggccg cgagaaacaa catgcgataa
     1201 tgggtgctgt attgcacggc ctcaggtaat tccacggcga tattggcgga
//
LOCUS       DAOPTR010001157         1249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_85743_length_1249_cov_2.409548, whole genome shotgun sequence.
ACCESSION   DAOPTR010001157 DAOPTR010000000
VERSION     DAOPTR010001157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85743_length_1249_cov_2.409548"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="PKI05_16470"
     CDS             <1..1126
                     /locus_tag="PKI05_16470"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOA53849.1"
                     /translation="LLAVLVVTVAAVILGSQLWHEIKQAEPSARLVLCQRLLLGGLGG
                     VLVILMFTVFIPRAVSLLWAAPVVYYAWPFWRFLERLMTPLLFVNRVVEVILARSAGV
                     QLPQESEEQFEEEIRTLVSEGEREGLLEGEAREMIEGVIKLSDVTVSEIMTPRTEMMC
                     LSAKLSWDEMLESVINEGHSRIPVYEETRDNIIGILYVKELLPYLARSAEQERPPWTS
                     LLREPFYVPETKPVDELMQEFQRTRNHMAIVLDEYGGVSGLITLEDILEEIVGEIGDE
                     LDQEVVEEIRIDDQGRAEVQGTARVEEVNRRLDLGLPESEEYDTIAGFLMTHLGRVPH
                     VGESIVFGKFRIKVLDGDRRRIRRVIIEPAEPVALPEAQN"
BASE COUNT          260 a          334 c          372 g          283 t
ORIGIN      
        1 gctcctcgcc gtcctcgtcg taaccgtcgc ggcggtcatc ctgggaagcc aactttggca
       61 cgaaatcaag caggcagaac cctctgccag actggtcctc tgccaaaggc ttctcctagg
      121 ggggctgggt ggtgtgctcg tcattttgat gttcaccgtg ttcatccccc gggccgtttc
      181 actgctttgg gccgccccgg ttgtctatta tgcctggcca ttctggcggt ttttagagcg
      241 actcatgacc ccattgcttt ttgtgaaccg agttgtcgaa gtcatccttg cccgcagcgc
      301 cggcgtgcaa ttaccccagg agtccgaaga acaattcgag gaggaaattc gcacgctcgt
      361 cagcgagggg gaacgggaag ggcttttgga gggcgaagcc cgcgagatga tcgagggcgt
      421 tatcaagttg tccgatgtca ccgtctccga gatcatgacg ccgcggactg aaatgatgtg
      481 cctctccgcg aaactgagct gggacgaaat gcttgagagc gtcatcaacg aaggacattc
      541 ccgtatccct gtttacgagg aaacccgtga taacatcatc ggaatccttt atgtgaagga
      601 actcttgcca tatttggcgc ggtccgcaga acaggaacgt ccgccttgga cgtcgcttct
      661 gcgcgagcct ttttacgtcc ctgaaaccaa acccgttgat gaacttatgc aagaattcca
      721 gcgaactcgc aatcacatgg caatcgtcct tgatgaatat ggcggcgtct ccggcctgat
      781 cacgctggag gacatcctgg aggagatcgt cggcgagatc ggcgacgaat tggaccagga
      841 ggtcgtggag gagattcgca tcgacgacca ggggcgggcc gaggtccagg gtaccgctcg
      901 tgtggaagag gtcaaccgtc ggctggatct tgggctgccg gaaagtgagg agtacgacac
      961 catcgcgggc ttcctcatga ctcacctggg gagggtccct cacgttggcg aatcgattgt
     1021 ctttggcaag tttcgcatca aggtcttgga tggtgatcgc cgccgaatcc gtcgggtgat
     1081 catcgaaccg gccgagccag tagcgttgcc cgaggctcaa aactgagttc gttcgctatc
     1141 tggtattgtg cttcaaggga atcatgtccg ggactgtggc aaggaaagga tgatcctttg
     1201 gaacgatatt acgcggctac ctgtcagaca aatttgccca atccggtcg
//
LOCUS       DAOPTR010001158         1246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_86073_length_1246_cov_2.245172, whole genome shotgun sequence.
ACCESSION   DAOPTR010001158 DAOPTR010000000
VERSION     DAOPTR010001158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86073_length_1246_cov_2.245172"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1246
                     /locus_tag="PKI05_16475"
     CDS             <1..>1246
                     /locus_tag="PKI05_16475"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOA53850.1"
                     /translation="MSRSILRIAACFLAVVVAVFNEHLLADQQPERPNIVWLTSEDNA
                     IFWYRLYNPDGAPMPNVERLAKEGLVFNNCYSCAPVCSPARSTIISGCYVPRLGAHWH
                     RKILPVTLPAGLHMFPWYLRQAGYYTTNNHKEDYNFAASEKADVWDESSPRATYRNRK
                     PGQPFFHVQNYTTTHESQLFPERPNDQLITDPKSVKLFPYHPDTPIFRNKYAHYFDLH
                     RQLDAQIGTFISQLEADGLLDDTFIFHFGDNGGVLPGSKQYAREDGLHVALVVYVPRK
                     WQHLVPAPRGSRINGVIEFVDLAPTVLNLAGIPIPPQMDGRPFLGPGVTLEELDQRDT
                     AFGHADRFDEKYDMVRFLRKGRFTYWRSYQPFNFDGLYNEYRYKQAAYREWRDLYEKG
                     QLNDTQKAFFEPRPPECLFDLE"
BASE COUNT          290 a          350 c          310 g          296 t
ORIGIN      
        1 tgatgagtcg gagcattctt cggatcgccg cctgcttcct tgctgttgtt gtggcggttt
       61 tcaatgaaca tttgcttgct gatcaacaac cggagcgacc caacattgta tggttaacaa
      121 gtgaggacaa tgctatcttc tggtaccgcc tttacaaccc tgacggggcg ccgatgccga
      181 atgtcgaaag gctagcgaag gaaggcctcg tgttcaataa ctgttattcc tgtgcgcccg
      241 tttgctcgcc ggcaaggagc acgatcattt cgggctgtta tgtcccccgg cttggtgccc
      301 actggcatcg gaaaatcctt cccgtcacgc tccctgcggg gcttcacatg tttccgtggt
      361 accttcgcca agccggttac tacaccacga acaaccataa agaggattac aattttgctg
      421 ccagtgagaa agccgatgtt tgggatgaat catctccccg tgcgacatac cgcaatcgca
      481 agcctggtca gccatttttc catgtacaga actatacgac cacgcacgaa agccaacttt
      541 ttccagagcg gcccaacgat caactgatca ctgatcccaa gtcggtaaag cttttccctt
      601 atcatccgga cacacctatc ttccggaaca aatatgcaca ttattttgat cttcaccggc
      661 aattggatgc acaaattggg acattcatca gccaactcga ggcggatggt ctgctcgacg
      721 acacgttcat ttttcacttt ggagacaacg ggggtgtcct tcccggtagc aaacaatacg
      781 cccgagagga cggactccac gtggcccttg tcgtctatgt gccacgaaaa tggcagcacc
      841 tggtcccggc gccgcgaggc tcgcggatca acggagtcat cgagttcgtt gacttggccc
      901 ctacggtgtt gaacctagcc ggtattccca tcccgccgca aatggacggg cgtcccttcc
      961 ttgggccagg cgtgacgctg gaggaactgg atcaacgaga cacggccttc ggtcatgccg
     1021 accgctttga cgagaaatac gacatggtgc gattcctccg taaagggaga ttcacctatt
     1081 ggcggagtta tcagccgttc aatttcgacg gcctttataa cgaatatcga tacaaacagg
     1141 cggcatatcg cgaatggcgc gatctttacg agaagggcca actcaacgac acacaaaagg
     1201 ccttctttga gcctcgccca cctgagtgtc ttttcgattt ggaaaa
//
LOCUS       DAOPTR010001159         1245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_86141_length_1245_cov_3.474790, whole genome shotgun sequence.
ACCESSION   DAOPTR010001159 DAOPTR010000000
VERSION     DAOPTR010001159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86141_length_1245_cov_3.474790"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..877)
                     /locus_tag="PKI05_16480"
     CDS             complement(122..877)
                     /locus_tag="PKI05_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM sorting domain-containing protein"
                     /protein_id="HOA53851.1"
                     /translation="MRWVHACGIFGTIVASLVYASPFEWAPEDYKPWSTEFPYQRNVD
                     LTFQVTPIRIPDGGPGDPGIPGAHYEGTADPILMLSDFVEFQGDFTWYDSVSGFPFQG
                     LIGIDNRNGNLPKGGSVVFHLDNFPIPNTEKKIWIEWDFLISAANVPISFYVSDSNGN
                     LAVEQWFGPIRTTQEGLSRQNIWFVLKPNPIWEEITILFPFVPAGGYALIDRFHVATE
                     CIPEPASGLLALVGCAGLAWFGVRRRRSRELLN"
BASE COUNT          321 a          364 c          317 g          243 t
ORIGIN      
        1 cgactaccag agtagcactc ggaaaggctg aaagaaagac ccacgccggg accttaaaga
       61 agaaaccccg cacatccggc ggggtttctc tgcacatcta aatggctcat caattcgatt
      121 tctaattgag cagctcgcgc gatcgcctac gacgtactcc gaaccacgcc agacctgcgc
      181 aaccgacgag cgcgagcaat ccactggcag gttcggggat gcactccgtg gccacatgga
      241 accgatcaat caacgcatag ccaccggcgg gcacaaaggg gaataggatc gtgatctctt
      301 cccatatcgg gttgggtttc agcacaaacc agatgttctg ccgtgacaat ccctcctgcg
      361 ttgtgcgaat gggtccaaac cactgctcta ccgcgagatt cccattcgaa tcgctcacat
      421 aaaaggagat cggaacgttc gcggcggaaa tgagaaaatc ccactcaatc caaatcttct
      481 tttccgtgtt agggataggg aaattgtcga ggtggaagac aacgctccca cctttcggta
      541 ggttaccgtt tctgttgtcg atgccgatta aaccctggaa gggaaaaccg cttaccgaat
      601 cgtaccaggt gaaatcgccc tggaattcga cgaagtcgct cagcatcaag atcgggtcgg
      661 ccgtgccctc ataatgagcc ccaggaatcc ccggatcacc cggcccaccg tcagggatcc
      721 gaataggtgt gacctgaaac gtgaggtcaa cgttgcgctg ataggggaac tcagtcgacc
      781 agggcttgta gtcttcgggt gcccattcaa atgggctagc gtaaaccaag gaggcgacga
      841 tcgtcccaaa aatcccacag gcgtgtaccc accgcatgat gccgctcctt ccacaaagtt
      901 gaatccacgc aaaccgctac agggcgcgca tcccctataa tcgcaccaga acacacatta
      961 tttatagcaa cgcgcagatc aatgtcaatc attttttcaa gttggccggg caatatcggg
     1021 ttaggttgga tactaccata gagggggtgc cgcacgccaa tctgaagtct gagcccgact
     1081 tccgcagtgt tatacgttcg gcctgcacaa gcacgacgtg ccgcccaaca gcgcatgggc
     1141 ttccgacagt tctcgcgagc cacccgtgca aaccagtggg cccaaaggca tgtccctgac
     1201 acagcttggg aaacgcacaa gtgataagga cacactcccg ccttg
//
LOCUS       DAOPTR010001160         1241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_86570_length_1241_cov_3.268128, whole genome shotgun sequence.
ACCESSION   DAOPTR010001160 DAOPTR010000000
VERSION     DAOPTR010001160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86570_length_1241_cov_3.268128"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..>1241)
                     /locus_tag="PKI05_16485"
     CDS             complement(105..>1241)
                     /locus_tag="PKI05_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOA53852.1"
                     /translation="VGKTIEEAGLRHLPGLYLAEINRDGQVLPAVSPQERLRANDRLI
                     FVGIVESVRDLQRIRGLVPATNQVFKLDAPRSDRCLIEAVISDRCPIVGKTIRDGRFR
                     SRYNAVVIGVAREGERLKGKIGDIVLRRGDTVLLEAPPSFVETHRHSRDFYLISPIEH
                     SKPPRYEKAGTALAILIAMVLVVSLSESLGDLTLMLGSLKIGLGRITMLKGALLAAGA
                     MLAFRCCTLSEVRRAIDWQVLVAIGASIGIGTSLDVTGAAQLVAGYVVDSVQHNPWLA
                     LLVIHAMTSLLTELVTNNAAAALMFPFVLATAQQLDVNPMPFIISIITAASASFATPL
                     GYQTNLMVYGPGGYRFTDYLRIGVPLNILVTLVTVTIAPLVWPF"
BASE COUNT          314 a          334 c          349 g          244 t
ORIGIN      
        1 aaaggaagtc ggctgcaaat tgtggctgcg ggtgttagca aggggcgtgg tcgcttgaca
       61 acacgcccca ccaagtcagg tcaccggaac tggtagcggt ctagctagaa tggccacacg
      121 agcggcgcaa tcgtaactgt gaccagggtc acaagaatgt ttaaggggac accgatccgc
      181 aggtaatcgg taaaacgata gcctccgggt ccatagacca tcaagttggt ttgataaccc
      241 aggggtgtgg caaatgatgc tgaggccgcc gtgatgatcg aaatgataaa aggcatgggg
      301 ttaacgtcca actgttgggc tgttgccaaa acgaaaggaa acattaaggc agccgccgca
      361 ttattggtca cgagctcggt cagcaaggac gtcatggcgt ggatgacgag tagtgccaac
      421 catggattgt gttgcacaga gtccacgacg taaccggcca ctaactgggc ggcacctgta
      481 acatctagtg aggttcctat cccgatggag gcaccaattg ccactaggac ctgccaatcg
      541 atggctcggc ggacctcgct cagggtgcag cagcggaagg caagcatggc ccccgcggcc
      601 agtagggcac cctttaacat cgtgatgcgt cccaggccaa ttttgagcga acctaacatg
      661 agggttaaat cgcccagaga ctcactaagg ctgaccacaa gcaccatggc gataaggatg
      721 gccagggccg tccccgcttt ttcatatcgg ggcggtttcg agtgctcaat ggggctgatg
      781 agataaaaat cccggctatg ccgatgggtt tccacaaagg acggaggggc ttctagaagg
      841 acggtatctc cgcgtcgcag gacaatatca ccgatctttc cttttaggcg ttctccctca
      901 cgtgctacac caataacaac ggcgttgtaa cgtgatcgaa aacgaccgtc gcgaattgtc
      961 tttcccacaa tcgggcagcg atcactaatc acggcctcga taagacaacg atcagagcgc
     1021 ggggcgtcca gcttaaaaac ttgatttgtt gcggggacaa gcccgcggat gcgttgcaaa
     1081 tccctcacgg attcgacaat tcccacaaag ataagacgat cattggcccg aagccgctcc
     1141 tggggcgaaa ccgccggcag cacctgacca tcacgattga tttccgccag atacaggccg
     1201 ggcaaatgac gaagtcctgc ctcctcgatc gttttcccca c
//
LOCUS       DAOPTR010001161         1240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_86669_length_1240_cov_3.516456, whole genome shotgun sequence.
ACCESSION   DAOPTR010001161 DAOPTR010000000
VERSION     DAOPTR010001161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86669_length_1240_cov_3.516456"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1240
                     /locus_tag="PKI05_16490"
     CDS             <1..>1240
                     /locus_tag="PKI05_16490"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOA53853.1"
                     /translation="SPWAAGVAVVLAASVLQRAIGFVRAAWFCRWLDAEQLGTWDLAF
                     SFLMTATPLVVLALPGCLGRYVGHFQERGMLGPFLRKVAFLQGGLSAFAGVVMVFLAE
                     PIAKLIFGSSGFASYVYLLAAGLVVVAWSNYLYELTTALRLSRWIATLQLINSVLFAV
                     FGIGLLWIGPRSAESVIIAYLVACAITIVCAYFVVRPWLLESHSNVTSQIGHVFWPKV
                     LWYSLGLWATGTVGNLFAVVDRYMIVHFSPLGVNALGQVGDYHAARLVPVLLVSLAGL
                     VATLSLPHLSHSWERNRRERCGEQLLFLVKVSGLLLMVVSTGVLIGRSVIFDTLLAGK
                     YPAAEEILPLTLLTCCWFSLFLLLENYVLCCEKAHWAAIAMVAGLLANVILNICWLPL
                     WGLWGAIAATAVANALALGVL"
BASE COUNT          186 a          329 c          354 g          371 t
ORIGIN      
        1 tcgccctggg ccgctggggt ggctgtcgtc ttggcggctt ctgttcttca aagggcgatt
       61 ggttttgttc gggcggcctg gttctgtcgc tggctcgacg ctgaacaact cggcacatgg
      121 gacctcgcct ttagtttcct gatgacagcc accccgctgg tggtcctcgc attacccggc
      181 tgtttgggac ggtatgtcgg acactttcaa gagagaggga tgctggggcc tttccttcga
      241 aaagtggctt ttcttcaagg aggattatcc gcttttgccg gcgttgtgat ggttttccta
      301 gccgaaccca ttgccaagct cattttcggc tcatccggct ttgcctccta tgtgtacctc
      361 ttagcggcgg gacttgttgt cgttgcctgg tcaaattacc tgtatgaatt gaccaccgca
      421 cttcgtctca gtcggtggat cgcgacatta cagcttatta atagtgtcct ttttgccgtc
      481 tttggtatcg ggctgctctg gataggaccc cggtctgcgg agtccgtcat cattgcctat
      541 ctggttgcct gcgccatcac gatcgtgtgt gcgtattttg ttgtccggcc atggttattg
      601 gaaagtcatt ccaacgtcac atcacaaata gggcacgttt tttggccaaa agtcctttgg
      661 tactcgctcg gtctttgggc caccgggacg gtgggcaacc tgtttgccgt tgtggatcgg
      721 tacatgatcg tccacttttc gcctctcggg gtgaacgccc tagggcaagt cggtgattac
      781 cacgcggcgc gactggttcc cgttcttctc gtttcccttg cgggactggt ggccacgctt
      841 tccttgcctc atcttagtca tagctgggaa cgcaatcgcc gtgaaagatg tggcgagcaa
      901 ttgctgtttc tcgtgaaggt ttccgggctt cttttgatgg tggtaagcac cggggtgctc
      961 atcggtcgtt cggtgatttt tgatacccta ctcgccggaa aataccctgc tgccgaggag
     1021 atccttccat tgacgcttct cacctgttgc tggttctcct tgttccttct gttggagaac
     1081 tacgtccttt gctgcgaaaa ggcccattgg gcggccattg ccatggtggc gggtttgctg
     1141 gcgaatgtta ttctcaacat ctgttggcta ccactttggg gcctgtgggg agccattgcg
     1201 gccacggccg tcgccaatgc tctggccctg ggagttttgg
//
LOCUS       DAOPTR010001162         1238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_86870_length_1238_cov_3.058326, whole genome shotgun sequence.
ACCESSION   DAOPTR010001162 DAOPTR010000000
VERSION     DAOPTR010001162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86870_length_1238_cov_3.058326"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PKI05_16495"
     CDS             complement(<1..200)
                     /locus_tag="PKI05_16495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53854.1"
                     /translation="MKRVKVRGDRDTKSYRGDGSADSPDGTQSIHERGLSRLLSQPII
                     LAIAGAALLWAALPPLDWWPLA"
     gene            complement(251..853)
                     /locus_tag="PKI05_16500"
     CDS             complement(251..853)
                     /locus_tag="PKI05_16500"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOA53855.1"
                     /translation="MIFILDNYDSFTYNLVQRLGELDNSLEIVVRRNDQVTVEEIERM
                     SPDRIIISPGPCTPKEAGISVECVRYFAGRVPLLGVCLGHQSIGEAFGASIVRAKRLM
                     HGKTDTIFHNGCELFEGLPNPFIATRYHSLVIDPPSLPDCFEVCSWSFLPDGEKEIMG
                     IRHKTFPLYGLQFHPESFLTEHGPTILANFLKLPSLAGRS"
BASE COUNT          298 a          338 c          318 g          284 t
ORIGIN      
        1 caggcgagcg gccaccagtc aagcggtggt aacgctgccc aaagaagggc tgctccggcg
       61 atggccaaaa taatgggttg cgacaaaagt cgtgataatc ctctttcatg aatcgattgg
      121 gtgccgtccg gcgagtcagc tgacccatcc cctcggtatg attttgtgtc gcgatcccct
      181 cttactttga cgcgcttcat ttcattcacg accctgactg acatttccgg gtcaaaatgc
      241 aagtggaaag tcaggaacga cccgccaaac tcggcagttt caaaaagttt gccagaattg
      301 ttgggccatg ctctgtaagg aagctctccg gatgaaattg aagtccgtac aagggaaaag
      361 tcttgtggcg gatccccatg atctcctttt caccgtccgg caaaaaagac catgagcaaa
      421 cctcgaaaca gtccggcaag gatggcggat cgatgaccag gctgtggtag cgagtcgcga
      481 tgaaaggatt gggcaatcct tcgaagagct cgcacccgtt gtgaaagatt gtgtcggtct
      541 tgccgtgcat gaggcgtttc gcccgaacga tggatgcccc aaaggcttca ccaatggatt
      601 ggtgtcccaa gcacacgcca agcaatggaa cccggccggc aaaatatcgc acacattcca
      661 ccgagattcc cgcttctttc ggcgtgcatg gtccgggaga gatgatgata cgatccggcg
      721 acatccgctc aatctcttcc actgtaacct gatcgttacg ccggaccaca atctccaacg
      781 agttgtcaag ctctcccagt cgttgcacga gattgtaggt gaacgaatcg taattatcga
      841 ggataaagat cattcccaac acctctctcg gatgatcata ccctgcctac tcttggggta
      901 cagtcgtgac tcgacttctg cccgctttcc taaggcaata gcccatgccg cagagggccg
      961 gcgccctccg tgttgtgcga tttccccatg ttaagcgtcc cgatcaacca tttgatagat
     1021 tttatcctac cggaccggcg tcgaggaacc agaacctgga tcacgtgatg ttgcaggcgg
     1081 ataatgccgt ttccaaccaa agaaaggccc ctgcgtggcg cgcctaagaa ggcgtgccta
     1141 cggaaataag tccattggcc caagcaaaag ttgaaatcga tctctggagt gcagctattt
     1201 ttgcttgatt tcttttttcg cggtgcgagg ccctaaca
//
LOCUS       DAOPTR010001163         1237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_86941_length_1237_cov_4.082064, whole genome shotgun sequence.
ACCESSION   DAOPTR010001163 DAOPTR010000000
VERSION     DAOPTR010001163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86941_length_1237_cov_4.082064"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PKI05_16505"
     CDS             complement(<1..389)
                     /locus_tag="PKI05_16505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53856.1"
                     /translation="MKHPQKALCRRAASFLSNSSAASTAEYALLLTLVIIVGAAGFFG
                     LSTFTRWVFQHNAQIASSSEPGSDRTSSSSTKPSETSFYLPHRIIERAIRTLDKAELA
                     IQFAAGLLGLAMGSGLVIVCWQAIHGRR"
     gene            complement(420..>1237)
                     /locus_tag="PKI05_16510"
     CDS             complement(420..>1237)
                     /locus_tag="PKI05_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008667051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOA53857.1"
                     /translation="SGRGLFLFGAPGNGKTSIAERVTAAFGPYIWIPRAIGVDGEIIR
                     LFDPVNHFEAPLPEPEGLLDTRHIDKRWVRIRRPTIIAGGELTMSALEVTVNRASGIC
                     EAPLQLKSNCGTLVIDDFGRQRMPIQELLNRWIVPLERRYDFLNLPNGKKITVPFDQL
                     IIFSTNLEPRQLVDEAFLRRIPYKIEVVDPTEEQFRQLFKILAPQLGIRYCEEAVDYL
                     IERYYKAVGRPFRFCQPRDLMLQILHYCNYQEIEPEMKKEYFDIAVANYFSVM"
BASE COUNT          297 a          319 c          352 g          269 t
ORIGIN      
        1 cgtctgccgt ggatagcttg ccagcagacg atcacaaggc cggaccccat cgccagtccc
       61 agcagacccg ccgcaaactg aatagccagt tcggccttgt caagagtacg gatggccctt
      121 tcgatgatgc ggtgtgggag atagaaactt gtttcagacg gcttggtcga ggagcttgac
      181 gtgcgatcgc ttccaggctc cgaagaggag gcaatctggg cattgtgttg gaatacccaa
      241 cgggtaaaag tagataaacc aaagaacccg gctgctccta cgataatgac cagggtcaag
      301 agcaaagcat attcagccgt gctcgcggct gatgagttgc ttaggaagct tgcagccctt
      361 cgacagagcg ccttttgcgg atgcttcatt tcggctggct cccgacctac ccacgggcct
      421 cacatgacgg agaagtaatt ggccacggca atgtcaaagt actctttttt catctccggt
      481 tcgatttctt ggtaattgca gtagtgcaga atctggagca tgaggtccct cggttgacag
      541 aagcgaaacg gacgaccaac ggccttgtag tagcgttcga tcaggtaatc cacggcctct
      601 tcacagtagc gaatgccaag ttgtggcgcg agaatcttga aaagttggcg gaattgctcc
      661 tcagtgggat caacaacctc aatcttgtac ggaatacggc ggaggaacgc ctcatcaaca
      721 agttgccgag gttccaagtt ggtggaaaaa atgatcaatt ggtcaaatgg gacagtaatt
      781 ttctttccat tgggcaagtt gagaaagtcg taacgtcgtt ccaaggggac gatccaccga
      841 ttgaggagtt cttggatcgg cattcgttgt cggccaaagt cgtcgatgac cagcgtccca
      901 cagttactct tgagctgcag gggggcttca caaatgccgg aggcgcgatt gaccgtgacc
      961 tccaaggccg acatggtgag ctcaccaccg gcgatgatgg tcggacggcg aatacgtacc
     1021 cagcgtttgt cgatatgccg cgtgtcgagg agtccctcag gttccggcaa gggggcttca
     1081 aagtgattga ccgggtcgaa gagccgaata atctcgccgt caactccaat ggcccttgga
     1141 atccagatat aagggccgaa ggccgccgtt acgcgttccg cgatgctcgt tttcccatta
     1201 ccgggtgcgc cgaacaaaaa caaaccccgc ccggagt
//
LOCUS       DAOPTR010001164         1237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_86947_length_1237_cov_3.579526, whole genome shotgun sequence.
ACCESSION   DAOPTR010001164 DAOPTR010000000
VERSION     DAOPTR010001164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86947_length_1237_cov_3.579526"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1237
                     /locus_tag="PKI05_16515"
     CDS             <1..>1237
                     /locus_tag="PKI05_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOA53858.1"
                     /translation="GWDEVDVVFVTGDAYVDHPSFAMALLGRLMEAEGYRVAILSQPD
                     WRSCEPWRQFGRPRLCFAVSAGNMDSMVNHYTANRKVRNDDAYSPGGQIGLRPDRATL
                     AYCQRAREAFKGVPVIAGGVEASLRRIAHYDYWSDKVRRSILLDSKADLLVYGMGERP
                     LLEILARLADGEDIKQLRDIRGTVYRLGASEPPVEDALMLPSYEEVVADKEAFARMTK
                     MAHLESNPYNARRLVQYHGREAVVVNPPAFPLTEEEMDRIYSLPFVRRPHPKYGDQPI
                     PAFEVVKHSIQIMRGCFGGCTFCSITAHEGRIIQSRSPDSILAEVNRIKEDRDFKGVI
                     SDIGGPTANMYRMNCSRPEVRAKCRRLSCLHPTICKLLDTNHGPLIDLMKRVRMVKGV
                     KIVFVASGIRTDLAQLSPAY"
BASE COUNT          263 a          309 c          386 g          279 t
ORIGIN      
        1 gggttgggac gaagtggacg tcgtctttgt caccggtgac gcctacgtcg accacccaag
       61 ttttgccatg gcgctgcttg ggcggctgat ggaggccgaa ggctatcggg tcgcgattct
      121 gagccaaccg gactggcgct cttgcgaacc gtggcggcag tttgggcggc ctcgcctgtg
      181 ctttgccgtg agtgcgggaa atatggactc gatggtgaat cactacacag ccaataggaa
      241 ggtccgtaat gacgatgcct acagcccggg cggacagatc ggattgcgtc ctgaccgcgc
      301 gaccctagcc tactgtcaac gggcccgaga ggcgttcaaa ggggtccccg tgatcgcagg
      361 tggcgtcgag gcgagtttgc gacgcattgc ccattatgac tattggagcg acaaggtccg
      421 ccggtcgatc ttgttagact caaaagccga cctgttggtt tatggcatgg gggaacggcc
      481 ccttctcgaa attctggccc ggttagcaga tggggaggat atcaagcagt tgcgtgacat
      541 ccgcgggacg gtctatcggc tgggggcgtc ggaaccgccg gtggaagacg ccttgatgct
      601 tccctcctat gaggaggtag tcgccgataa agaggctttt gcccgaatga caaaaatggc
      661 gcatctggaa agcaaccctt acaatgctcg gcgtttggtg caatatcatg ggcgggaggc
      721 ggtcgttgtg aatcctccgg cgtttcctct cacggaagag gagatggatc gtatttacag
      781 cctacctttt gtgagacgac cgcatccgaa gtacggtgac cagccgattc cggcctttga
      841 ggtggtcaag cattccattc agattatgcg aggatgtttt gggggatgca cattttgctc
      901 gatcacggca catgaagggc ggattattca aagccgttcg ccggattcta ttcttgctga
      961 agtcaaccgc atcaaggagg atcgagactt taagggagtg atcagcgaca taggcggccc
     1021 cacagcgaat atgtatcgaa tgaattgcag tcggccggag gtgcgggcaa aatgtcgtcg
     1081 attaagttgt ctccatccca ccatttgcaa gttgctcgac acgaaccatg gaccactgat
     1141 cgatcttatg aagcgagtcc gaatggtgaa aggggtcaag atagtctttg ttgcgtcggg
     1201 gattcgcacg gatctggctc agctcagccc tgcctac
//
LOCUS       DAOPTR010001165         1237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_86960_length_1237_cov_3.056684, whole genome shotgun sequence.
ACCESSION   DAOPTR010001165 DAOPTR010000000
VERSION     DAOPTR010001165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86960_length_1237_cov_3.056684"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(228..>1237)
                     /locus_tag="PKI05_16520"
     CDS             complement(228..>1237)
                     /locus_tag="PKI05_16520"
                     /inference="COORDINATES: protein motif:HMM:NF021041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HOA53859.1"
                     /translation="SLKEYGRIIPTCDCHLDAEKEKEIVDSDTAGTGLALLPFLGAGV
                     TPESAPEEPAELARYRNGVRRGLSYLMRIQVNDPKSDKDGHLGGNTYAHAIATMALCE
                     AYGLTGDERIQTAAQRAIKYLMSSQHKEGGWRYGFREAGDMSVVGWVFLAIRSGQLAG
                     LKIDESPLVRAGRFLDSCGVGPAPYNLSEYCYQPGGEPKISLTACGLLTRQYLGWKKD
                     NPHLLAGVKKIMTQLPPAGAQNVGSLYYYYYATQVLHHMEGEEWDLWNHRMREHLIRT
                     QERSGHRAGSWSPEGCDWGNRGGRIYATSLALMILEEYYRHLPLYRPVPRTQLTGTTN
                     R"
BASE COUNT          247 a          362 c          355 g          273 t
ORIGIN      
        1 cggggccttg accggttgga attccggcgc cgtggtgatg tacacgtaat agggatcgtt
       61 ctcgtcagca aatgaaaccg ccgaaagcgt cagagagacg gttaccgcaa ggcacatgct
      121 taagcgtgtt gccataatag gacctccgga ctacctggtt gcgggagtcg ggcgatgcgg
      181 aaatgcacgt cgcttttgca acgactattg taacggatgg gccggcgtta gcgatttgtg
      241 gtccccgtca attgtgtccg tgggacaggc cggtaaagcg gaaggtgccg gtagtattct
      301 tccaggatca tcagggccag ggatgtggcg taaatccgtc cgccccgatt gccccaatcg
      361 cagccctcgg gactccagct tcctgcgcga tggccggagc gctcttgggt gcggatcagg
      421 tgttcccgca tgcggtgatt ccaaaggtcc cattcctcgc cttccatgtg atgaagaacc
      481 tgcgtggcgt agtaatagta gtaaagcgac ccaacgtttt gtgccccggc gggcggaagc
      541 tgggtcatga tcttcttcac accggccaaa agatggggat tgtccttctt ccatcccaag
      601 tactgccggg tcaggagccc gcacgccgtc aaggagatct tgggttcgcc gcccggttga
      661 tagcaatact ccgataggtt gtagggtgcc ggtcccacgc cgcagctatc gaggaagcgt
      721 ccggctcgaa cgagtgggct ttcgtcgatt ttaagaccgg ccaattggcc gctccggatc
      781 gccagaaaga cccaacccac caccgacata tcgccggctt cacgaaaacc gtagcgccag
      841 cccccctctt tgtgctgaga actcatgagg tacttaatcg cccgttgggc cgccgtctga
      901 attcgttcat ctccggttag cccataagcc tcacaaagag ccatcgtagc gattgcatgg
      961 gcgtaggtgt ttccgcccaa atggccgtct ttatcggact tgggatcgtt tacctggatt
     1021 ctcatcagat acgacaggcc acgccgtacg ccattacgat aacgcgccaa ttcagccggt
     1081 tcttccgggg cactctccgg cgtaacccct gcgccgagga acggcagcag ggccaagcca
     1141 gttcctgccg tatcgctatc cacgatctcc ttttcttttt ccgcatcgag gtggcaatcg
     1201 caggttggga tgatgcggcc atactccttc agcgacc
//
LOCUS       DAOPTR010001166         1237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_86974_length_1237_cov_2.671743, whole genome shotgun sequence.
ACCESSION   DAOPTR010001166 DAOPTR010000000
VERSION     DAOPTR010001166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86974_length_1237_cov_2.671743"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PKI05_16525"
     CDS             <1..491
                     /locus_tag="PKI05_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53860.1"
                     /translation="TIVMWVRGVDGGSLLDKREWLMRFEKAVVFTLVFAIVYAVGVGC
                     QKAGEKPQATKENEAHPMEIANQPSPGALPSTTESQPGTASVQQAASDQLPVAEAAKA
                     GDAEVGTQESAASEDDEGISLGRMVGGVFRAARSVVPLPVSASPDRRTEPDDQAPAFP
                     QQ"
BASE COUNT          290 a          293 c          398 g          256 t
ORIGIN      
        1 ggaccattgt tatgtgggtg cgaggggttg acggtggaag cctgttagac aaaagggaat
       61 ggctcatgcg ttttgaaaaa gcggtcgtgt tcactctcgt tttcgcgata gtatacgctg
      121 tgggagttgg gtgtcagaag gcgggagaga agccccaagc caccaaggaa aacgaggctc
      181 atccaatgga aatagcgaat cagccatcgc ccggagccct ccctagcaca acggaatctc
      241 agcccggtac agcaagtgtg caacaggccg cgagcgacca gttgcctgtt gcggaagcgg
      301 caaaagcagg ggatgcggaa gtcgggactc aagagagcgc cgcatctgag gatgatgagg
      361 gcatctccct ggggcgcatg gtgggtggag tattccgagc ggctcgctcg gtggtgccgc
      421 tcccggtttc ggcatcgcca gatcgacgca cggagcctga cgaccaggca ccggcttttc
      481 cccaacagta gatcattcac gacgtgacca gcctatccca aaaagtcccc ctctcccttt
      541 gggagagggt tggggtgagg gcaagcgaac gtcggaattg atagagaaaa acagcagcga
      601 ttcgagcacg ctcacccccg accgccctcc tgccgaacgg gtcagggaag attatgggat
      661 aggctctaag ttggaccgat aggtatcgcc ggtcaaggga ctaaaagtcg ttagccgatt
      721 ggggttccgg cggaccaacc acggttcatc tcgtggataa cgcgttggta attatcgtag
      781 gcgcggcgat aaggaatctt ggaatcgcgg ccgtccgtct tttctggttc tttttggaac
      841 aagcgcttcc tattgggcct ggagaactca tttgtagcaa tttgcaggtg tgattaggga
      901 aacgctataa taaggcacag gtgctgggtg gaagcgctat tcttacgttg tgaggacttg
      961 cctgcgtatt tgtcggcgaa agatgcctgt ccacgagaaa ggttaatggg aacggctctc
     1021 ggccgtgaaa acgtgtgagt ctcattttct gaaatagcga cgggcgtcga tgagtccgga
     1081 aatgagatcg gaggtgcgca atgagcctca atggagatgc gcgacgcagt tcaggcggag
     1141 ccggaaatga gaatcaggac gccgacgcac ggctggtaga gctaacggaa ctgagggcca
     1201 cgcgacttgg ctttccattt gtggatgtgc gtaaagt
//
LOCUS       DAOPTR010001167         1236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_87057_length_1236_cov_3.209145, whole genome shotgun sequence.
ACCESSION   DAOPTR010001167 DAOPTR010000000
VERSION     DAOPTR010001167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87057_length_1236_cov_3.209145"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="PKI05_16530"
     CDS             complement(<1..1127)
                     /locus_tag="PKI05_16530"
                     /inference="COORDINATES: protein motif:HMM:NF040466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdjY domain-containing protein"
                     /protein_id="HOA53861.1"
                     /translation="MSFAQRKQLGLVKHQPWLLGFAVTTALFLLTGGCREPSVADRPS
                     PSAARKAPATKFDGEAGAKTATKSEGLTTSAETYDHLDIAIDAPTEWTVGDEVSIQLT
                     LSNHSDHTIEGTELRAEIQFGLQDSISEGHIVVPIASLPGKTSYHSMANFVSSRPGIW
                     YVKAAVYLQGELIKATTRTLYIREATVRQEPKPADDTDESLGPPLVDHPERLQRLHPD
                     FPIWIDAEGHALVMIGRVCQREVPLELFACLKNSKEHESIVSINTRAYIVHAGLLALG
                     VEPGRPVQFYPQYRPAEGPEIDIVVAWKDAAGNIHRMPAQQWVRHAQTKEPLELSWIF
                     TGSQIVKDEDTGDQYYYADSTGELICVSNFPSAVLDLPIRST"
BASE COUNT          263 a          337 c          358 g          278 t
ORIGIN      
        1 gtacttcgga tgggcaggtc caggacggcc gacggaaaat tggaaacaca aatcaattca
       61 ccggtgctat cagcgtaata atactgatct cctgtgtctt cgtccttgac gatttgacta
      121 cccgtgaaga tccaggaaag ctcaagaggc tctttagttt gggcatggcg gacccactgt
      181 tgggcgggca tgcgatggat gttaccggcc gcatctttcc acgccacaac gatgtcgatt
      241 tcaggccctt ccgcggggcg atactgggga tagaactgca ccgggcgtcc cggttcgacc
      301 ccgagggcca aaagacctgc atgaacaata taggctcgag tgttgatcga gacgatggac
      361 tcgtgttcct tggagttctt aaggcaggca aatagctcca aggggacttc tcgctggcag
      421 acccgaccga tcatgacgag ggcatggcct tcagcatcga tccagatggg gaaatccggg
      481 tgaagccgtt gcaatcgctc cgggtgatcg accagcggag gccccagcga ctcgtcggta
      541 tcatcagccg gttttggctc ctgtcgaacg gtggcctcgc ggatgtaaag ggttcgcgtt
      601 gtagccttga tcaactcacc ctggagatac acggcggcct taacgtacca aatccccgga
      661 cgggatgaaa cgaaattggc catcgaatgg tacgacgtct tgccgggcaa ggaggcaatg
      721 ggcaccacga tgtggccttc gctgatacta tcctgaaggc cgaactgaat ctccgcgcgt
      781 agctccgtgc cctcaatggt atgatcactg tggttgctca gggtgagttg gattgagacc
      841 tcgtctccga cggtccattc cgtgggggca tcaatggcga tatccagatg atcgtaagtc
      901 tccgcggagg tggtaagccc ttcggacttg gtggccgtct tggccccggc ctcgccatca
      961 aacttggttg ccggagcttt tcgtgcggca ctgggtgatg gcctatctgc caccgagggt
     1021 tcccgacatc cgccggttaa caggaaaagc gctgttgtta ccgcaaatcc caatagccac
     1081 ggctgatgtt tcacaaggcc gagttgcttc cgttgtgcga acgacatcgg ttctcctcga
     1141 tacgaagtct tcccaaactg accctgtgga acctatgggg acgtacagtc gtttttcaag
     1201 cagcaaaccc ccgttggggc atcctatcat gattct
//
LOCUS       DAOPTR010001168         1235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_87196_length_1235_cov_2.170339, whole genome shotgun sequence.
ACCESSION   DAOPTR010001168 DAOPTR010000000
VERSION     DAOPTR010001168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87196_length_1235_cov_2.170339"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PKI05_16535"
     CDS             complement(<1..310)
                     /locus_tag="PKI05_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOA53862.1"
                     /translation="MFVDEVEIFVQGGRGGDGCVSFRREKYVPKGGPDGGDGGDGGSV
                     ILVAEEGVDSLALLAHRKHWIAEDGERGGPADRHGRSGEDLIIPVPPGTLVFDAEHNL
                     L"
     gene            complement(426..677)
                     /gene="rpmA"
                     /locus_tag="PKI05_16540"
     CDS             complement(426..677)
                     /gene="rpmA"
                     /locus_tag="PKI05_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOA53863.1"
                     /translation="MAHKKGQGSSRNGRDSNAKYRGVKRFGGQFVKAGSILVRQVGTR
                     FQPGLGVGVGRDYTLFALTDGYVKFDRGGRRVNVVPSLN"
     gene            complement(746..>1235)
                     /locus_tag="PKI05_16545"
     CDS             complement(746..>1235)
                     /locus_tag="PKI05_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53864.1"
                     /translation="RPVLEDDGTYRIESVDVPVLFIPTFYLEGKAASGEKRYPDRVIP
                     LAKPIIQAREDRAIQFFDSTETARTLQVGQSMWGVAMWEDVDPTINCFSIIVAGLSNA
                     YKWTDPPGAYKVGDPVGQGRVFQRKMLKINFWRPGDEFIVKEEYVRLGQPGAVDYEWI
                     YR"
BASE COUNT          299 a          401 c          277 g          258 t
ORIGIN      
        1 ggaggaggtt atgctcggca tcaaagacaa gcgttcccgg cgggacggga atgataaggt
       61 cctcacctga tcgcccatgc cggtcggcag gcccaccacg ctcgccgtcc tcagcgatcc
      121 aatgtttgcg gtgtgccaat agggccagac tgtcgactcc ctcctccgca acgaggatga
      181 cacttccccc atctccccca tccccgccat ccggtccccc cttggggacg tacttttccc
      241 gccggaagct aacacatccg tctccgccac gccccccttg aacgaaaatc tcaacctcat
      301 ccacaaacat tgtcagcgaa catgttgaaa aatggcggcc gacatcgtgg agctacggaa
      361 ataccgcgca caaaaaagga gaccaactcg gtctcctaaa gcagccttcc aacgaaatcg
      421 aacaatcaat tcagcgaggg gacgacattt acccgacggc ctccccgatc gaatttcaca
      481 tacccatcgg tgagggcaaa cagcgtatag tcacgcccaa cgccaacacc taaaccaggc
      541 tgaaaccggg tcccaacctg tcgcacgagg atgctcccgg ctttgacaaa ctgccccccg
      601 aaccgcttca caccccgata cttggcgttt gagtcgcgtc cgttacggct cgaaccttgt
      661 cccttcttat gcgccatggc ttacacccat tccatatcga atcaaatgga gaactttcac
      721 tttacaacca ctatagaact catttttacc ggtaaatcca ttcgtagtca accgccccgg
      781 gttgtccaag gcggacgtac tcttccttaa cgataaactc gtcgcctggt cgccaaaaat
      841 tgatcttgag cattttcctt tggaagacgc ggccttgacc gacgggatcc ccgaccttgt
      901 acgcccctgg cggatccgtc catttgtagg cattgcttaa ccctgccaca ataatcgaaa
      961 agcaattgat cgtcggatcg acatcttccc acatagccac accccacatg gactgcccaa
     1021 cctgcaaggt gcgggctgtc tcggtggaat caaaaaattg aatagcccga tcctcccgtg
     1081 cttgaatgat gggctttgcc aatgggatga cgcgatcggg atatcgcttc tcgccacttg
     1141 ccgccttgcc ttcgaggtaa aacgtcggga tgaataagac cggcacatca acactctcga
     1201 ttcgataggt cccatcgtct tccaggacag gacgc
//
LOCUS       DAOPTR010001169         1233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_87380_length_1233_cov_2.837861, whole genome shotgun sequence.
ACCESSION   DAOPTR010001169 DAOPTR010000000
VERSION     DAOPTR010001169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87380_length_1233_cov_2.837861"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="PKI05_16550"
     CDS             <1..904
                     /locus_tag="PKI05_16550"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HOA53865.1"
                     /translation="QAKSYMQTMLVASGCKRLIEQGKTTSIRGLFYLLKHTIEGTREE
                     TFADQDECDPIIEDIEVTLNALREELHVYATNRGAMVGEITIRDSGDEIDCTRMGSGG
                     YAIPSIVEPNVIQFVRCDAKFVLHVEKDTVWRRFNEDKFWKKHKCILTHGMGQPPRGV
                     RRLLHRLHYELGLPIYCLLDNDPWGYYIYSVIKQGSINLAYESQRMAIPDARFLGLRS
                     TDFERCGLSDSVTIALTDADRKRAKQIMEYPWFKQKKAWQAEIQKLLDNGFKLEVEAL
                     ISKDISYVTEVYTPQRLRDGDWLD"
BASE COUNT          291 a          324 c          358 g          260 t
ORIGIN      
        1 tcaggccaaa agctatatgc agaccatgct ggtggccagc ggttgcaaac ggctcatcga
       61 gcagggaaaa acgaccagca tccgtgggtt gttctatctg ctcaagcaca ccatcgaggg
      121 tacgcgggag gagacgttcg ccgaccagga cgagtgcgat cccatcatcg aagatattga
      181 agtgacgcta aacgctcttc gggaagaatt gcacgtctat gccaccaacc gcggggctat
      241 ggtcggggaa atcaccattc gtgacagcgg agacgaaatc gactgcacgc ggatgggctc
      301 gggtggctac gccattccat cgattgtgga accgaatgtg atccagtttg ttcgatgcga
      361 tgcgaagttc gttctccacg tcgaaaagga cacagtttgg cgtcgcttca atgaagataa
      421 gttctggaaa aagcacaagt gtatcctgac ccacgggatg ggtcagcccc cgcgaggtgt
      481 gcggcggctg ttacaccgcc ttcattatga attgggatta cctatctatt gcctcctcga
      541 taatgacccc tgggggtact acatctacag cgttatcaaa caggggtcga tcaatctggc
      601 gtatgagtcg cagcggatgg ccatcccgga tgcccgcttt ctgggcctac gttccacgga
      661 tttcgaacgc tgcggccttt cggatagcgt cacaattgcc ctgacggacg cagaccgcaa
      721 acgggcaaag cagatcatgg aatatccctg gtttaaacag aaaaaggcct ggcaggcgga
      781 aatccaaaaa cttctcgaca atggcttcaa attggaggtt gaggcactca tctccaaaga
      841 cattagctac gtcaccgagg tttatacccc ccagcgactc cgcgacggag attggctcga
      901 ttgaaaagat cgcccctaac gggtacttgt ggtcaagatt tagcggaatg gagaccgcgt
      961 gcgcccggcg cggcatcaga ggaagacgaa cttcggtcat gagccacccc tgcgctggtt
     1021 ctccatggtt cggttgtgac cgggcgcggt ccagggcacc cacacggttc cggtaggcaa
     1081 ggcaagccag gcagtttttc ttggcggtgg ctgtcagtgt ggcagtccgt ttttgtgcgt
     1141 agagaacgct cctaatcacc gatcggccag tacgctggag ggatcaggca gggattctac
     1201 ggttttggaa ggcgttaagc gcgacatacc cac
//
LOCUS       DAOPTR010001170         1231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_87588_length_1231_cov_3.428571, whole genome shotgun sequence.
ACCESSION   DAOPTR010001170 DAOPTR010000000
VERSION     DAOPTR010001170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87588_length_1231_cov_3.428571"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PKI05_16555"
     CDS             complement(<1..234)
                     /locus_tag="PKI05_16555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53866.1"
                     /translation="MSKKQTNNHSNNPEHVKEEHLQNATPDRPESDARNSASVQEERD
                     TNEVPTAEAEVMSDEQIQQPTPEQNAGTISAEDY"
     gene            complement(330..>1231)
                     /locus_tag="PKI05_16560"
     CDS             complement(330..>1231)
                     /locus_tag="PKI05_16560"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOA53867.1"
                     /translation="GAYQPHEFRDVSEIFDLFSNFFGDDLFGDIFGGFGRGRRPRRGD
                     DIICELTLELHEAALGTTKSVTFERHTICGRCRGTGARPGTQPERCRYCGGRGRVGQS
                     AGFITIQTTCPACQGRGVVIRENCPECRGKGVVREVVTREVRIPPGVDQNTRLRLQGE
                     GELSLDGGPRGDCYCLIRIKEHPLFKRDGANLVCRIPISYAQAALGATIEVPTLDGPE
                     TLTIPPGTQSEQVFVLPGKGMPTRSRHRGNLLVQVYIEVPKKLTPKHERLLRELAELE
                     NNEVLPERKSFFAKLKEYFAGGS"
BASE COUNT          237 a          369 c          314 g          311 t
ORIGIN      
        1 gtaatcctct gctgagatcg tcccggcatt ctgttccgga gttggttgtt gaatctgttc
       61 gtctgacatt acttccgcct ctgctgttgg gacttcgttt gtgtctcttt cttcctgaac
      121 ggatgccgaa ttccttgcat ccgattcagg ccgatcagga gtcgcgtttt gaagatgttc
      181 ttctttgaca tgctctgggt tattcgaatg attgtttgtt tgcttcttgc tcatggtcgg
      241 ctcctttcca gtcacctagg ttgagttttc gacccaaggg tatccgcccc aactttgact
      301 gagggaagga gcttttaact ttgcagtctt tacgacccac cggcaaagta ctctttaagc
      361 tttgcaaaga agctttttct ttccggcaac acctcgttgt tttctaactc ggccaattca
      421 cgtagcaacc gttcgtgctt gggagtgagt ttcttaggca cctctatata aacctgcaca
      481 agcaggttac cgcgatggcg tgatcgcgtc ggcatccctt tgccgggtag cacgaaaact
      541 tgctcgcttt gcgtccctgg ggggatcgtc aacgtttctg gcccatcgag cgtcggtacc
      601 tcaatcgtgg cccccagcgc cgcctgggca taactgatgg gaatccggca gaccaagttg
      661 gcaccatctc gcttaaatag cggatgctcc ttaatgcgga ttaagcaata acaatcaccg
      721 cggggtccac catccaggct caattccccc tcaccttgca accgcaaacg ggtattttga
      781 tccacgcccg gtgggatacg gacctcccgg gtgacaacct cccggaccac gcccttgccc
      841 cggcactcgg ggcagttttc tcggataaca acccctcgcc cctggcaggc cggacaggtc
      901 gtctgaatcg tgataaagcc cgccgattga ccaacgcgcc cccggcctcc gcagtatcgg
      961 caacgctcgg gttgtgtgcc cggtcgggca ccggttcctc gacatcgccc gcagatggta
     1021 tggcgttcaa acgtgacact ctttgtggtg cccagagccg cctcgtgcag ttcgagcgtc
     1081 agctcgcaaa tgatatcgtc cccgcgacgt ggccgccgcc ctcgaccgaa acccccgaaa
     1141 atatcgccga aaaggtcgtc tccgaaaaag ttgctgaaga ggtcgaatat ctcgctgacg
     1201 tcacgaaatt cgtgcggctg ataagccccg g
//
LOCUS       DAOPTR010001171         1230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_87692_length_1230_cov_3.186383, whole genome shotgun sequence.
ACCESSION   DAOPTR010001171 DAOPTR010000000
VERSION     DAOPTR010001171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87692_length_1230_cov_3.186383"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PKI05_16565"
     CDS             complement(<1..379)
                     /locus_tag="PKI05_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53868.1"
                     /translation="MNTGKSFCRVGPLVIGLCILILPGTLGEPVTEIVPVAQFAAPAL
                     GAESSPFSPLPEPEAPSTPVGHTGGTSPPKTTAPNVLPQDGTRGQSPSTAAQSGASVE
                     IEREAKPSSSSLSFWRIVRAGGTV"
     gene            complement(567..>1230)
                     /gene="xseA"
                     /locus_tag="PKI05_16570"
     CDS             complement(567..>1230)
                     /gene="xseA"
                     /locus_tag="PKI05_16570"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOA53869.1"
                     /translation="QAVRRVNDLALPIDVIVVTRGGGSAEDLGAFNTEAVVRAIATSR
                     IPVVSAVGHDIDVTLADLAADVRALTPTDAAFKVIPSREEVTEAVNALAVRLRSSAEL
                     RLRMGQAMFERTANHPFFRRPKDWLYEKVQTLDEWDKRLDRAGHLIIERFSQQLQQKI
                     THLESLSPLAVLARGYSMTFVHPDGPLVVEASALRPGQQIKTRFAQGSAVSRIEEVLA
                     SS"
BASE COUNT          274 a          344 c          375 g          237 t
ORIGIN      
        1 ctaccgttcc accggctcgc acaatccgcc agaacgaaag cgaagaagaa ctaggcttgg
       61 cctcccgctc tatttcgacg ctcgcaccac tttgggcggc tgtggagggt gattggcccc
      121 tggtgccgtc ctggggtagc acattcggag ccgtggtttt tgggggactc gtacccccag
      181 tgtggccaac gggagttgaa ggggcctcag gctcaggcaa tggcgaaaaa ggtgatgatt
      241 ccgcgcccag ggccggagcc gcaaattgtg ccacggggac gatctcagtg acgggttcgc
      301 ctagtgtccc agggaggatg agaatgcata aaccgatgac gaggggccca acccggcaaa
      361 atgatttgcc cgtgttcaca agtgttaaaa acctttgccc agaggaaacc atcgaccagt
      421 agcctttgtt ccgaaaatca tgaaacggta gtgactgagt aaatgacgca agaggcgtaa
      481 atgattcggg gacacgccta accacttatg gtagcaatat cctgcccgca cgttgaggcc
      541 attcatccca tgccccgtgg gtagtgttac gatgaggcaa ggacctcttc gatccggctg
      601 acggcactgc cttgggcaaa gcgggttttg atctgctgcc ctgggcgaag ggccgacgcc
      661 tcaacgacaa gagggccatc ggggtggaca aatgtcatgg aatagccccg ggcgagcaca
      721 gccaaaggac tgagggattc caggtgggtg atcttttgtt gcaactgttg tgaaaaccgt
      781 tcgatgatca aatggcccgc acgatccagg cgtttgtccc actcatcgag cgtctggact
      841 ttctcgtaaa gccagtcctt gggacggcga aagaaaggat ggtttgccgt ccgttcgaac
      901 atggcttgcc ccatccgtaa tcgcaattcg gcactcgatc gcaaacggac ggccagggcg
      961 ttgaccgctt ccgtgacctc ttcccgggac ggaatcactt taaaagcggc gtcggttgga
     1021 gtcagggcac gaacatccgc cgccaggtcc gccaacgtga catcgatatc gtggcccacg
     1081 gccgagacga cgggaattcg cgatgtggcg atggcgcgaa cgaccgcctc ggtgttgaag
     1141 gccccaaggt cctcggcgct gccaccgccg cgggtcacaa caatgacatc aattggaagc
     1201 gcgaggtcgt tcacccgccg gacagcctgg
//
LOCUS       DAOPTR010001172         1229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_87804_length_1229_cov_2.966780, whole genome shotgun sequence.
ACCESSION   DAOPTR010001172 DAOPTR010000000
VERSION     DAOPTR010001172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87804_length_1229_cov_2.966780"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(884..>1229)
                     /locus_tag="PKI05_16575"
     CDS             complement(884..>1229)
                     /locus_tag="PKI05_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOA53870.1"
                     /translation="QTIELVAKRLNNRLRLTGIILCMFDSNTRLAAEVSEDVGAFFGN
                     HRERKSPWAGAHMFQTRIRRNIRLAEAPSFGQSIFQYSPDSNGAEDYRNLAREVIQQM
                     PVAENSSFKKAC"
BASE COUNT          298 a          376 c          311 g          244 t
ORIGIN      
        1 cgataacagc cagtgctggc ctggggcgat agtgaggata gtgggtgccc tggggcagtt
       61 tcaggaggta cgactcccat gcccctcccg tctggtcgcc ccacgacacc gacctctcgt
      121 ccgccttccc gaggctccgg cgcggccgtg agaccggaat gtcccccatt taacggggca
      181 agccaaccgg tgtgcatcgg gggaccttgc tcctgttggg gctcacttat accaccgccg
      241 ccttgctgcg aattgtgaaa ccctatgcca gacgatgggg gagacttcag ccagggcaac
      301 agatcagggc tggcactcag tccgaactgc cccaaagcca ctattggcct agcgagaaga
      361 acaaccgcca ccaacccgca tatcaacgcc aatcggacgt tcatgggcat cgagtcgctt
      421 cggaaatcat ataggaaacg gggcaccgaa agtcacgcgg ctttgcgcca aacggcaact
      481 cagcgcgcgg gccacggatt ctcgccaaat aaagggtcgt cggggctgaa tttcgttgga
      541 cctttcagaa aaaccaataa tgcacgcaaa ctgtaaccgt tccgtgcatt agggagaatc
      601 gtcaagaaaa gcctaatccc accggcaaac aagattagtc gaaaaacatt gcccacctgt
      661 caaacctggc gcccatcggc taaagatggc cgattgcagg ccgatactct atcggtatca
      721 cccgtcaaag cgggtaactc gacaaacctt cgttccccgg aaaagttcgt cgaatcgtca
      781 caggctaaac tgcgcaccag gaaacaaagc ccacggtgtc aacaaacaca actgcgagcc
      841 cctttttttc gttcgccgcg accgtgtcaa ctttgcagcg cgatcaacac gcctttttga
      901 aggacgaatt ctcggcaacc ggcatctgct ggatcacctc ccgggccaag ttgcggtaat
      961 cttcggcacc atttgaatcg ggcgaatact ggaagatcga ctgaccaaaa ctgggtgcct
     1021 ccgccaaccg aatgtttcgg cgaattcgtg tttgaaacat gtgtgccccc gcccacgggc
     1081 tttttctttc ccgatgattt ccgaaaaatg cccccacatc ttccgagacc tcggccgcta
     1141 accgggtgtt gctatcaaac atgcagagaa tgatacccgt caaccgcagc cgattattca
     1201 accgcttggc caccaattcg atggtctgt
//
LOCUS       DAOPTR010001173         1225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_88238_length_1225_cov_2.334188, whole genome shotgun sequence.
ACCESSION   DAOPTR010001173 DAOPTR010000000
VERSION     DAOPTR010001173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88238_length_1225_cov_2.334188"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="PKI05_16580"
     CDS             <1..1205
                     /locus_tag="PKI05_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOA53871.1"
                     /translation="REDGRANPNQLFFRPENKEAMRQLLNHLKDAHRERFGAHAVDDL
                     LVGLQLTHSGRFCRPRRKDLPEPLIAYHHPVLDQKFGIDPTNDNVVLKDEDIPPLIDA
                     YVEAAKMAEEVGFQFVDVKACHGYLGHEFLSAFDRPGPYGGDLMGRSRFLREIITAIR
                     SECPRLMIGVRMSIFDFPPFRPDPNQSREGKLGPGIPHDYPIPYPGFGCDRQNPLAID
                     LREPIELLRLLHRDYGVMLVNLTAGSPYYNPHIQRPALYPPSDGYQPPEDPLVGCLRQ
                     IHAVKQIKQAIPQLVVVGSAYTYFQEFLPQVAQGVIRAGWTDFVGIGRLVLAYWDLPA
                     DTLAGRPMQTKRLCRTFSDCTTGPRNGLISGCYPLDPYYKELPEFQALRSFKSAMRAK
                     SGDSSEAS"
BASE COUNT          249 a          346 c          338 g          292 t
ORIGIN      
        1 tgcgtgagga cggtcgggcc aatcccaatc agctattttt ccggcccgag aacaaggagg
       61 ccatgcgaca gcttcttaat cacctaaaag acgcacaccg cgaacggttt ggtgcacatg
      121 ccgttgatga ccttttggtg ggtctgcaac tgacgcattc tggtcgtttt tgccggccaa
      181 ggcggaagga cttgccagag ccactgattg cctatcatca cccggtgtta gatcagaagt
      241 tcggcatcga tccgaccaat gacaacgtcg ttctcaaaga tgaagatatt cctcccttga
      301 tcgatgccta tgtcgaggct gccaagatgg cagaagaggt cggctttcag tttgtcgatg
      361 tgaaggcgtg ccacggctat ttgggacacg agttcttgtc ggcctttgat cggcctgggc
      421 catacggtgg ggatttgatg gggaggtccc gctttctgcg ggagatcatc acggcgattc
      481 ggtccgaatg cccacggctg atgatcggtg ttcgcatgtc aatcttcgat tttccgccgt
      541 ttcggcctga cccgaatcaa agtcgggaag gaaagctcgg gccagggatt ccccacgatt
      601 atcctatccc ctatcccggg ttcggctgcg accgacagaa tcctctggcc attgacctcc
      661 gcgaaccgat tgaattgcta cgtcttctcc atcgggatta cggagtcatg ttggtcaatc
      721 tgacggcggg atcaccttac tacaatccac acatccagcg gcccgcgctt tatcccccct
      781 cggacggtta tcaaccgcct gaagatcccc tggtcgggtg tttacggcag attcacgctg
      841 tcaagcagat caaacaggcg atccctcagc ttgtcgtggt gggttcggcc tacacgtatt
      901 tccaagaatt ccttccccaa gtcgcacagg gagtcattcg ggcaggctgg acggattttg
      961 tgggaatcgg acgtctcgtc ctcgcgtatt gggatttgcc ggccgacacc cttgccggcc
     1021 gtcccatgca gacgaagcga ctctgtcgca ccttcagcga ctgtaccacg gggcctcgca
     1081 acgggcttat cagtgggtgt tatccgcttg acccctatta caaggagttg cccgagtttc
     1141 aagccctccg atctttcaag tcggcaatgc gggcaaaatc gggtgactct tcggaagcgt
     1201 cgtagtcaaa gcaactgcgc gagat
//
LOCUS       DAOPTR010001174         1224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_88338_length_1224_cov_2.122327, whole genome shotgun sequence.
ACCESSION   DAOPTR010001174 DAOPTR010000000
VERSION     DAOPTR010001174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88338_length_1224_cov_2.122327"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PKI05_16585"
     CDS             complement(<1..320)
                     /locus_tag="PKI05_16585"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOA53872.1"
                     /translation="MADRHDVVSAGILFADVACEPIERLPRAGELIPTKRIQLGLGGC
                     AANVAVDLKKLGISVGVSGCVGDDFFGQFISQVLDREGVDTGGIRSVPGMTTAATMMV
                     NVKG"
     gene            complement(418..1050)
                     /locus_tag="PKI05_16590"
     CDS             complement(418..1050)
                     /locus_tag="PKI05_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HOA53873.1"
                     /translation="MAKVLITSGPTREYLDPVRYLSNASSGRMGRALAEVFLEHGHQV
                     FIVSGPVEIEYPNGADVVPVITTEEMLQECQRRFEQCDGLIGAAAPCDYRPMTFSPEK
                     LRKSGHTLAVELVETPDILATLATRKGSRWIVGFALETHDVHNRAMEKLRKKQCDLIV
                     INRPAAIGAQTTEVEIADANGVILGNWTGSKEAIARRLYEIVAQRFLRRG"
BASE COUNT          296 a          370 c          275 g          283 t
ORIGIN      
        1 tcacctttca cgttaaccat catcgttgcc gccgtggtca ttccgggcac agaacgaatc
       61 ccacctgtat ccacaccctc gcggtcaagc acctggctga tgaattgccc aaagaaatca
      121 tcaccaacgc agccgcttac acccactgag atgccgagct ttttcaggtc cacggcgacg
      181 ttcgcggcac accctcccaa gcccagttga atccgctttg tggggattaa ctctccagca
      241 cgaggcaatc tctcgatcgg ttcgcaagcc acatcggcaa acaaaatacc cgcggataca
      301 acatcatggc gatcggccat ggagcatctc ctcaccttgg tcccaagtct cacgattaac
      361 agaagacatt atttcaccga accgcttttc ggtggtcaag acgccgccaa acgcttctta
      421 tccccttctg agaaaacgtt gggcaacaat ttcgtacagc cgtcgggcaa tcgcctcctt
      481 ggaacctgtc caatttccca aaataacgcc gttggcatct gcaatctcaa cttcagtggt
      541 ctgtgccccg attgcggctg gtcggttaat gacgatcaaa tcacattgct tttttcgaag
      601 tttttccatc gcgcggttgt ggacatcatg ggtctccaag gcaaacccca caatccagcg
      661 agaccctttc ctcgtggcaa gcgtcgcaag aatgtcaggc gtctcgacaa gttccaccgc
      721 aagggtgtga cccgatttgc gcagcttctc gggggaaaaa gtcataggac ggtaatcgca
      781 aggcgcggct gccccgatta ggccgtcaca ttgttcgaaa cgtctttgac attcttgcaa
      841 catttcttct gtcgttatga ccggcaccac atccgcgcca ttggggtatt caatctcaac
      901 aggacccgaa acgatgaaca cctgatggcc atgctcgaga aacacctcgg caagggccct
      961 gcccatccgc ccacttgagg catttgagag ataccgcacc ggatcaaggt actcgcgggt
     1021 gggtcctgag gttatcaaca cttttgccat gatctcattc caatcacagg cgatgactac
     1081 aaacattcaa gaacgaaaaa tctaacaccc tgccctgctg gtacacccga cacctttttt
     1141 actccgcacg tctcagggtg gatttcactt cctggtggca ggtaatcgct caataggccg
     1201 atcattgttg aatcttagaa acgg
//
LOCUS       DAOPTR010001175         1221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_88590_length_1221_cov_3.384220, whole genome shotgun sequence.
ACCESSION   DAOPTR010001175 DAOPTR010000000
VERSION     DAOPTR010001175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88590_length_1221_cov_3.384220"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..786)
                     /locus_tag="PKI05_16595"
     CDS             complement(205..786)
                     /locus_tag="PKI05_16595"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HOA53874.1"
                     /translation="MLGILLILLAVASMTDVLWGRIPNKITYPGIISGLLLNVLGTLA
                     VRLGWARAEHLSALGWIGMGQSVLGFLACGLVMLACYVFFRIGGGDVKLIAMLGTFLG
                     PDRGITAMLWTFVLGACLGLIALVWRVGPWRLLVLAGRHLLWSIRLGRWDPLTPEERA
                     QLQPPLFLAPSALAATVIVGFELVERYVLAVGP"
     gene            complement(860..1066)
                     /locus_tag="PKI05_16600"
     CDS             complement(860..1066)
                     /locus_tag="PKI05_16600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53875.1"
                     /translation="MKKASLWQLLRQIHADDRGAVSLETILIIGAIALPILIFLIKVG
                     WPRIKGYFTQGLQELEAERANAIQ"
BASE COUNT          328 a          415 c          277 g          201 t
ORIGIN      
        1 taaggctttg cacagccccg gcttcgtcac ggtggagagt taagagccgg ctgacattgg
       61 cttggggctt ttgcgcccgc agaagaagcc acgcaacagc gcatgccgcc aggaggactc
      121 cggcccaagg aagacaggca atcaaagtgg ctcggagcat agacctaccc tctctcaccg
      181 acagagagag cttatccagg ccgactacgg ccccacagca aggacatacc tctcaaccaa
      241 ttcaaatccc acaatcacag tggcagccaa ggcactcggt gccaaaaaaa gtggaggttg
      301 aagttgcgct cgttcctccg gtgtcaacgg gtcccaacgt ccgagacgaa tcgaccacaa
      361 aaggtggcgt cccgccagca cgaggagccg ccacggtcca actcgccaca ccagggcaat
      421 cagtcccaaa cacgccccca ggacaaacgt ccacaacatt gccgtgatgc cccgatctgg
      481 tccgaggaag gtccccaaca tggcgattaa ctttacgtca cctcccccga tccgaaaaaa
      541 cacataacag gccagcatga ccagcccgca cgccaggaaa cccaacaccg actggcccat
      601 gccaatccag cccaacgcac tcaaatgctc ggccctcgcc caacccaacc gcacagccaa
      661 tgtgcccaag acattcagca acaagccgct gatgatccct gggtaggtaa tcttgttcgg
      721 aatcctcccc cacaaaacat ccgtcatcga tgccacagcc aacaggatca agaggattcc
      781 gagcacggga cctcctcagg tcaagccaca tctccagggc acataccaca tgccggcggc
      841 aatcttgcga tgcctaccgt cactgtatgg cgttggcccg ttcggcttcc aattcctgaa
      901 gtccctgcgt gaagtaaccc ttgatcctcg gccaacctac tttgatcagg aagatcaaaa
      961 taggcagggc gatggccccg atgatgagga tcgtttccaa actgacggct ccccgatcat
     1021 cggcatgaat ctgtcgcaac aattgccaca aactcgcttt cttcataggc tgctcctcac
     1081 acacccttct ctgttgccgg aaactcacaa accgcaaacc ctaccttatc acataaaggg
     1141 ccagccaatc aacacgtaca ccatctgata agtcaaccag ataagccgcg gacccgccca
     1201 catacaaaac gccgcgacgg g
//
LOCUS       DAOPTR010001176         1221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_88598_length_1221_cov_2.849914, whole genome shotgun sequence.
ACCESSION   DAOPTR010001176 DAOPTR010000000
VERSION     DAOPTR010001176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88598_length_1221_cov_2.849914"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..696
                     /gene="frr"
                     /locus_tag="PKI05_16605"
     CDS             136..696
                     /gene="frr"
                     /locus_tag="PKI05_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOA53876.1"
                     /translation="MTEEEILFTVEEKMEKAISVLKENLAGIRTGRANPGLVESIKVE
                     AYGTTVPLKQLAAVGVPEPQQIVIRPYDPTTLKDIEKAIQAADLGFNPQNDGRVIRVN
                     VPPLSTEVRKKMVARIRELCEEAKIAIRNIRREGNKMADQAEKDKILSEDDCERVKER
                     IQELTKEYEEKANELAKHREKDVMEG"
     gene            794..>1221
                     /locus_tag="PKI05_16610"
     CDS             794..>1221
                     /locus_tag="PKI05_16610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53877.1"
                     /translation="MASEQNGLSLPEPPPSPQAALLRPRLREPVWPIFLVVVLSLVVQ
                     VMGLGLIGLVAVLRYPEARESADALRIVLTQATDEPQAILIGASGVMMGFGLLAFFAA
                     SLSRHPIRETLRLAKPAGDAGVIVLAIVGVLSLQTAFIAAG"
BASE COUNT          256 a          328 c          373 g          264 t
ORIGIN      
        1 accctacggt aagctcggtg gctggtgatt ccttttgcgg attgttacaa tcggcggggt
       61 attggcagca tgtcggggtc gttggatgtg ttgtgatgag cgggatgttt ctttcgcgga
      121 tggaaaggcc ttcgcatgac ggaagaagaa atccttttta ccgtcgaaga gaaaatggaa
      181 aaagcgatct cggtcctcaa ggagaacctg gcggggattc gcaccggccg cgccaatccg
      241 ggtttggtcg agagcatcaa ggttgaggcc tatggcacga ccgtgccgct taagcaattg
      301 gccgccgtgg gggttcccga gccgcagcag atcgtcattc ggccttatga cccgactacg
      361 cttaaagata tcgagaaggc tatccaggcg gccgatctcg gctttaatcc gcagaacgat
      421 ggccgcgtca ttcgcgtcaa cgtcccgccc ctttctaccg aagtccgcaa gaagatggtt
      481 gcccgcatcc gggaactatg cgaggaggcc aagatcgcca ttcgcaacat ccgccgggag
      541 ggcaacaaaa tggccgatca ggcggaaaag gacaagatcc tttccgagga cgattgtgaa
      601 cgggtcaaag agcggattca ggaattgacc aaggaatacg aggaaaaggc caacgaattg
      661 gccaagcacc gcgagaagga cgtcatggaa ggttaatggg tcacaaaccg cttttgcggc
      721 acatgagcgg agatttctcg ccgggcatct gatacgacaa agcattgacc gtcttctgag
      781 acggtgttcc gtgatggcgt ccgagcaaaa cggattatca cttccagaac ccccaccgtc
      841 gccacaggcg gcattgctcc ggcctcgcct ccgcgaaccg gtctggccga tttttctcgt
      901 ggttgttctt tccctggtcg tccaagtgat ggggctaggg ctgatcggtc tggtggccgt
      961 gttgcgctat cccgaagcgc gagagtctgc cgacgcgctt cgaattgtcc tcactcaagc
     1021 cacagatgaa ccgcaggcga tcctcattgg ggcaagtggt gtgatgatgg gattcgggtt
     1081 gctggccttt ttcgccgcgt cgctttcccg gcaccctatc cgcgagactc ttcgccttgc
     1141 caaacctgcg ggagatgctg gggtgatcgt gctggccatt gtgggcgttc tatcgctgca
     1201 aaccgcgttc atcgccgccg g
//
LOCUS       DAOPTR010001177         1220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_88735_length_1220_cov_2.151931, whole genome shotgun sequence.
ACCESSION   DAOPTR010001177 DAOPTR010000000
VERSION     DAOPTR010001177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88735_length_1220_cov_2.151931"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="PKI05_16615"
     CDS             complement(<1..1057)
                     /locus_tag="PKI05_16615"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOA53878.1"
                     /translation="MTTRRGRVRMSALVLVFFVVSSIAGLAADRPGPNPKAVEEVLAG
                     QRSEANAAWWGFDPEDSTEYLQAAINSGASRVIIPNMGQDWIVRPIFLRSNLELVLED
                     GTVVTAKKGEYHGKRDSVFTAQDVENLIIRGYGATIRMHKEDYAAPPYEKAEWRMGIN
                     IRGAKNVEILGLTIRDTGGDGIYLGRGTWRLHNENIVIRDVIADNNYRQGISVITVKD
                     LLIENSVFKNTGGTNPMAGIDFEPNHRDEILQNIVVRNCVFENNKGPGIVLALANLDR
                     WAKVSFFFENVLIKGASVGFSVTARHPDNHAAGEITVRNCLIEDTARQGIGLRYIDGD
                     TLKVRFEDCIVHNVATSS"
BASE COUNT          260 a          379 c          293 g          288 t
ORIGIN      
        1 ccgaagaagt ggctacattg tgtacaatgc aatcctcaaa gcggacttta agcgtgtcgc
       61 cgtcgatgta acgcagaccg ataccctgcc gtgcggtatc ctcgatcaga cagttgcgca
      121 ccgtgatctc acccgcggca tgattgtccg gatggcgagc cgtgacgcta aagcccactg
      181 aagctccttt gatcagcacg ttttcgaaaa aaaacgagac cttggcccag cgatccaggt
      241 tggccaaggc gagcacaata cccggcccct tgttgttttc gaatacacag ttgcgaacca
      301 cgatgttttg cagaatctca tcccggtgat tgggctcaaa atcgatgccg gccatgggat
      361 tcgtcccgcc cgtgttctta aagacactgt tttcaattag cagatctttg accgtaataa
      421 cgctgatacc ttggcggtag ttgttgtccg cgattacgtc tctaataaca atattctcat
      481 tgtgaagtct ccacgtacca cggcccagat agatcccgtc accgcccgtg tcgcggatgg
      541 tcaaccccag gatttctacg ttctttgcgc cccggatgtt gatccccatg cgccattcag
      601 ccttctcgta gggcggcgcc gcatagtctt ccttgtgcat gcgaatggtg gccccatagc
      661 cccggatgat cagattctca acatcctgcg ccgtgaacac cgaatccctc ttaccgtgat
      721 actcaccctt tttggcggtc accacggtcc catcttccaa gacgagctcg agattgctgc
      781 gcaagaatat gggacgcact atccagtcct gacccatgtt ggggatgata acccgggagg
      841 cgcctgagtt gatggcagcc tgcaggtact ccgtggaatc ctcggggtca aatccccacc
      901 aggccgcatt ggcctcgctg cgctgtcccg ccaaaacctc ttcgaccgct ttcggattcg
      961 gccccggcct gtcagcggcc aaaccggcga tcgacgacac aacaaaaaag accagcacaa
     1021 gggcggacat ccgtaccctt ccccggcgcg tcgtcatttt ttgatcctcc ttaagatcct
     1081 tgtgcttcca gaatctcccg caccaaagcg gcgttttcct cgactatgtc ctctttgtcg
     1141 cccctatgca tgcctacgtt gaccacatcc cccggtttga tgtgctcaaa ggcatactca
     1201 aacgccatgc gtgggtcaat
//
LOCUS       DAOPTR010001178         1218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_88951_length_1218_cov_2.169390, whole genome shotgun sequence.
ACCESSION   DAOPTR010001178 DAOPTR010000000
VERSION     DAOPTR010001178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88951_length_1218_cov_2.169390"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PKI05_16620"
     CDS             complement(<1..275)
                     /locus_tag="PKI05_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53879.1"
                     /translation="MEVTGYHGQRLVNSYYNGDGTVGTLTSPPFTLQRHYLAFLIGGG
                     GYHDETYMELVVDGQSVRRAVGPNTAPGGSEELDWAVWDVRELEGKTA"
     gene            complement(342..470)
                     /locus_tag="PKI05_16625"
     CDS             complement(342..470)
                     /locus_tag="PKI05_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53880.1"
                     /translation="MRRIAVGLFLVALIALAGFPWAGAEDAVVRPDIVLDDFERET"
     gene            complement(467..>1218)
                     /locus_tag="PKI05_16630"
     CDS             complement(467..>1218)
                     /locus_tag="PKI05_16630"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOA53881.1"
                     /translation="ELVSEIRSGLERTNGNEPLFCPRYMRLVLLALAIAAFNQLSGIN
                     AILYYAPRIFQMAGAGKYTAFLQSVAVGGTNLIFTMLAMSVIDRFGRKFLMLVGSIGY
                     ILSLSAVATAFYIYGAQFDTFGNIVVLVSLIVFIASHAFGQGAVIWVFISEIFPNTVR
                     AKGQALGSFTPVYVRSHFMDISRHCRTIGWACLCVLRGHDGPAIVVGHIHHARNKRHP
                     PRRDRTKTFNKAVTIFATRVIIRPFWMEARP"
BASE COUNT          296 a          355 c          323 g          244 t
ORIGIN      
        1 gcggtttttc cttccagttc gcggacgtcc caaacggccc aatcgagctc ttccgatcca
       61 ccgggagcgg tgttcggtcc cacggcgcgg cgaacacttt ggccatccac caccagttcc
      121 atgtaggttt catcgtgata tccgccacca ccgatcagga aagccagata gtgccgttgc
      181 aaggtgaaag gtgggctggt caacgtgccc accgtgccgt cgccgttgta ataagaattg
      241 accaagcgtt ggccatgata gccagtgacc tccatttgtc gtggaagagt gccggcggca
      301 ggccccggcc caaacgcctc accctcagca atccacggtg cttaggtctc ccgctcaaag
      361 tcatccagta cgatatcggg ccgcacgacg gcgtcttctg cgcctgccca agggaagcca
      421 gctagagcga tgagcgccac cagaaagaga cccactgcga tccgcctcat ggccttgcct
      481 ccatccaaaa tggcctaata atcacacggg ttgcgaagat cgtgaccgct ttgttaaaag
      541 tctttgttcg atctcttcta gggggatgcc ttttgtttcg ggcatgatga atatgaccca
      601 caacaattgc aggaccatca tggccgcgta gaacgcaaag gcatgcccac ccgattgttc
      661 ggcaatgacg ggaaatgtcc atgaaatgac tgcgcacata aaccggggta aaactcccca
      721 gggcctgccc cttggcccgg actgtgttgg ggaagatttc gctgataaac acccaaatga
      781 cggccccctg cccaaacgcg tgggaggcga taaacacaat taagctcacg agcacaacga
      841 tgttgccgaa tgtgtcgaac tgcgctccat agatgtaaaa tgctgtagcc accgcggaaa
      901 ggctcagaat gtagccgatg gagccaacga gcatgagaaa cttccgcccg aagcggtcga
      961 tgacgctcat ggccaacatt gtgaagatga ggtttgttcc cccgactgcc acagactgga
     1021 ggaaggccgt atatttgccc gcccccgcca tttggaaaat tcgcggcgcg taatacaaga
     1081 tggcattgat ccctgacaac tgattgaaag cggcaatcgc cagggcgaga aggacaagcc
     1141 gcatataacg ggggcaaaac agtggctcat tgccattcgt tcgctctagt ccgctacgaa
     1201 tttcgctaac cagctcct
//
LOCUS       DAOPTR010001179         1214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_89360_length_1214_cov_2.543572, whole genome shotgun sequence.
ACCESSION   DAOPTR010001179 DAOPTR010000000
VERSION     DAOPTR010001179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89360_length_1214_cov_2.543572"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PKI05_16635"
     CDS             <1..389
                     /locus_tag="PKI05_16635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HOA53882.1"
                     /translation="ESVVPDLIAIDLWPFRIQQIKVQSLTTQECPACHLGQYDYLSTV
                     GLSLTTELCGQKSVQITPPCPAKCDLDHLSQQWSTLGTVERSPFFLRLRSAGWVLTLF
                     PDGRAIIDGINDVALARSIYAQFVGM"
     gene            complement(443..>1214)
                     /locus_tag="PKI05_16640"
     CDS             complement(443..>1214)
                     /locus_tag="PKI05_16640"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOA53883.1"
                     /translation="RWFRNSDVVMMRTDWSDQAIYVGFKGGDNSVNHSHLELGSFVLD
                     GLGERWVLDLGPDDYNLPGYFGSQRWTYYRLGTEGQNTLLFDGQNQNPRAKAPVLACY
                     DTGVRKHAVVDLTAAYNPGNETASAKDSVQRGIAILGKDWVVVQDEIMGHAGQTVQWQ
                     IHTEAKVQVRGKVAELALGGKTVKVFLLSPEKAVFQTLSANPSRPQRQNPGITRLAVQ
                     TPVPQNGMTIAVAFQFNPSAKAEAPKIVPLKEWPGQIR"
BASE COUNT          272 a          342 c          307 g          293 t
ORIGIN      
        1 tcgagtccgt ggtgcctgac ctgattgcca ttgatctttg gccgttccgc atccagcaaa
       61 tcaaggtcca gtcactgaca acgcaagaat gtcctgcgtg tcacttgggg cagtatgatt
      121 acctttccac ggtaggattg tctctgacaa ccgaattgtg tggccaaaaa agtgtgcaga
      181 tcacaccacc ttgcccggcc aagtgtgacc tggatcacct aagtcaacaa tggagtacac
      241 taggaacggt ggaaaggtcc ccgttctttc tgcggttacg gtcggctggg tgggtcctga
      301 cattattccc cgacggtcgc gccattattg acggcatcaa cgatgttgcc cttgcacgat
      361 cgatttatgc gcaattcgtc gggatgtgat ccagcgacgg gaacctttcc ttcctatctc
      421 aaatggaacg tcggtagcac ttttatcgga tctggccggg ccactctttc aggggaacaa
      481 tcttcggagc ctccgctttg gcactggggt tgaattgaaa cgctacggcg atcgtcatcc
      541 cgttttgggg caccggcgtt tgaaccgcta atcgggtgat gccggggttc tgtcgctgag
      601 gccgggaagg gttggccgaa agggtctgaa acaccgcctt ctccgggctg agcaagaaga
      661 ccttaacggt ctttcccccc aacgccaact cggccacttt gccacgaact tgaaccttcg
      721 cctcggtatg aatctgccac tgcaccgttt gacccgcgtg tcccataatt tcatcttgga
      781 caacgaccca gtccttgccc aagatagcta tcccacgttg cacagaatcc tttgcggaag
      841 ccgtttcatt gcccgggttg taggcagccg ttaggtcaac tacggcatgt tttcggacac
      901 cagtatcata acacgcaagc acaggggctt tcgctcgggg attttgattt tggccgtcaa
      961 agaggagcgt attttggcct tcggtgccca aacgatagta cgtccatcgt tgcgatccga
     1021 aataaccggg taagttgtaa tcatcaggcc caaggtccag aacccaacgt tcgccaaggc
     1081 catccaacac aaaacttccc agttccaaat ggctatgatt gaccgagtta tccccgcctt
     1141 tgaaccctac ataaatggcc tgatcagacc agtcagtccg catcatgacc acatctgagt
     1201 tacgaaacca gcgg
//
LOCUS       DAOPTR010001180         1213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_89456_length_1213_cov_3.184801, whole genome shotgun sequence.
ACCESSION   DAOPTR010001180 DAOPTR010000000
VERSION     DAOPTR010001180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89456_length_1213_cov_3.184801"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            386..>1213
                     /locus_tag="PKI05_16645"
     CDS             386..>1213
                     /locus_tag="PKI05_16645"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOA53884.1"
                     /translation="MVSRRVVIVGGVAGGASCAARLRRLDENAEIFVFERGGFVSFAN
                     CGLPYYIGGVIAPRERLIVASPERFRDYFNIEIRVHHEVTKIDRENRIVHVRNCQTGT
                     SFTRPYDNLVLSPGARPIVPNVPGVDLPGVFTLRLLEDAERIYRWLEDRQVTRAVVVG
                     AGYIGLEMVENLVHRNIAVTLVEAANQILPGFDGEMVVPFYDKLAAHEVEILLGSPLC
                     AIEPHNSQLAVVAGGKRIPCGAVILCVGVRPEVTLAENAGLEIGELGGIRVDEQMRTT
                     "
BASE COUNT          239 a          288 c          363 g          323 t
ORIGIN      
        1 acggagaata agcctgcaga acattgtttt ttccctgttg atactagcta tactcatccc
       61 tgcggcccta agcggagtta tgagcgttgc ttttgcggtt ttcgttcatg aggcctccga
      121 acttttggcg gtggcgaacg gtctccgtat ggcaaggggt tgaaaccggt tgtgacaaca
      181 gcttggcttg ctcatgagac atgccataag ctaggagcgg cctaatgatg tgctatctgg
      241 attcttgcac attgctatgt ccattattct gtggcgactg ccataccgtg gggacagttc
      301 tttcccgaaa gttataattc gtttttatgg cagtttagcc actacacgtg tgtcggttct
      361 tggattctag cagaaggagt gatgaatggt ttcgcgccgg gtggtaattg ttggtggcgt
      421 ggctggaggc gcttcctgtg cagcccggtt gcgacgctta gacgaaaatg ccgaaatctt
      481 cgtcttcgaa cgtggcggtt ttgtgtcctt tgctaattgt gggttgccgt attacatcgg
      541 cggggtgatt gcgccgcggg aaagacttat tgtcgcctcg cccgagcgat tccgtgacta
      601 tttcaacatc gaaatccgtg ttcatcacga ggtgaccaag atcgatcggg aaaatcgtat
      661 tgtgcatgtc cgaaattgcc aaacgggaac atccttcaca aggccgtacg acaacctcgt
      721 gctgtcgccg ggggctcgcc ccatcgttcc caatgtaccc ggcgtggatt tacctggggt
      781 gttcaccttg cgtctgcttg aggatgcgga gcggatttat cgctggctgg aagaccgcca
      841 ggtaacgcgt gccgttgtgg tgggcgctgg gtatatcggg ctggaaatgg tggaaaacct
      901 cgttcaccgc aatattgccg taacccttgt ggaagctgcc aatcagatcc ttcccggctt
      961 cgatggcgaa atggtggttc ctttctacga taaattagcc gcccatgagg tcgaaatcct
     1021 cttaggcagc ccgctttgcg cgattgagcc gcacaattct caattggcgg ttgtcgcggg
     1081 tgggaagcga attccctgcg gtgcggtgat tctttgtgtg ggagttcgcc cagaggtgac
     1141 gctggcagag aacgccggat tggagatcgg cgagctgggt gggattcgcg ttgacgagca
     1201 aatgcgaaca acc
//
LOCUS       DAOPTR010001181         1213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_89458_length_1213_cov_3.143351, whole genome shotgun sequence.
ACCESSION   DAOPTR010001181 DAOPTR010000000
VERSION     DAOPTR010001181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89458_length_1213_cov_3.143351"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PKI05_16650"
     CDS             complement(<1..595)
                     /locus_tag="PKI05_16650"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HOA53885.1"
                     /translation="MPRVDRWMIYGAYGFTGSWIAREAVRRGLRPILAGRNASRLVPL
                     ADELNTEYRTFSLDEPNVVREALQDCRLVLNCAGPFSQTAQPMVMACLETGTTYLDIT
                     GEVDVLEWIAQLHESATRRNVTLIPGLGFDVVPTDTLAVLLKEAFPSANLLQLALTGT
                     GSLSVGTTRTIIEACQKGGRIRRNGQIVPVPLGWKKRR"
     gene            complement(716..>1213)
                     /locus_tag="PKI05_16655"
     CDS             complement(716..>1213)
                     /locus_tag="PKI05_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53886.1"
                     /translation="RVHPSEIPSLMPKSVTLYDPVVRLRADMSDPRITEMDIPWHFTN
                     IQRGLQVARQLLANQDTPNRQIILITDGLPTAHFEDRYLYLMYPPHDRTEEATLREGL
                     LCAREGIVINIFLLPGWTQSREDVRFAYKLAESTRGRVFFTAGKDLDRYVVWDYLERR
                     REIIG"
BASE COUNT          286 a          318 c          344 g          265 t
ORIGIN      
        1 cacgtcgctt tttccagccc aacgggaccg gaacgatctg cccgttgcgg cggatccgac
       61 cacctttctg acaggcttcg atgatggtgc gggtggtccc aacgctgagt gagccagtcc
      121 ctgttaaggc aagttgcaag aggttggctg acgggaatgc ctctttcagc agtactgcca
      181 atgtatcggt agggacaacg tcgaacccca gacctgggat caatgtgaca ttccggcggg
      241 ttgccgattc gtggagttgg gcaatccact ccagcacgtc cacctcgccg gtgatgtcaa
      301 gataggttgt tcctgtttcg agacaggcca tcaccatggg ctgagcagtt tgggaaaaag
      361 gtcctgcaca atttaaaact aggcggcaat cttgcagggc ttccctgaca acgttgggtt
      421 cgtccaacga gaacgtccga tactcagtgt tcagttcgtc cgctagagga acaagacgag
      481 aggcgtttcg accggcgagg atgggccgaa gaccacgacg gacggcctcc cgcgcaatcc
      541 aggaccctgt gaatccgtat gcaccataga tcatccaccg atcaacccga ggcatagtta
      601 ttcaccatta gttttgacat ccagtgaggg ccgccgtgag aaaccgttgc cttcgtccaa
      661 tgtatctcaa atacggagcg gtagcgaagc ggcgtccgga attatcgaga agcggttagc
      721 cgataatctc tcggcgtcgt tccagatagt cccacacaac ataacggtcg aggtcttttc
      781 cggcggtgaa gaagacacgg ccacgagtcg actcagccag tttgtaggca aaccgaacat
      841 cctcacggga ttgtgtccat cctggcaaga gaaagatgtt gattacaatc ccctcgcggg
      901 cacacagcag accctcccga agtgtcgcct cttctgtccg gtcatgaggt ggatacatga
      961 gatacaaata gcgatcctca aagtgggcag tgggtaatcc gtcggtgatg agaatgatct
     1021 gccgatttgg ggtgtcctga tttgcaagaa gctgccgtgc cacttggagt ccacgttgaa
     1081 tattcgtaaa gtgccagggg atatccattt ccgtgatgcg aggatcactc atgtcggcac
     1141 gcaaccgaac gacggggtca tataacgtaa ccgatttcgg cataagtgat gggatttcgc
     1201 tgggatggac gcg
//
LOCUS       DAOPTR010001182         1213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_89459_length_1213_cov_3.136442, whole genome shotgun sequence.
ACCESSION   DAOPTR010001182 DAOPTR010000000
VERSION     DAOPTR010001182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89459_length_1213_cov_3.136442"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PKI05_16660"
     CDS             <1..327
                     /locus_tag="PKI05_16660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53887.1"
                     /translation="SIPVAADRFDVVAEVTLPKKPLPLVPAMTGTIRFNVYVKEDALV
                     VPAKAVFTDELDPDKRYVYVLVDGKPEKRSVKVGEQVRDKVEILSGLRDGEEILLEKP
                     QESSGT"
     gene            394..>1213
                     /locus_tag="PKI05_16665"
     CDS             394..>1213
                     /locus_tag="PKI05_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOA53888.1"
                     /translation="MTTRFSVVGVSCILLVTWFGHSVPQLHAEEVTSSVSPLATTGPK
                     PKPIEAVSPEAVERAIRRGVDFLLLRQNRDGSWGSARNTKSLNIYAPVPGAHQAFRAA
                     VTSLCVSALIDVKDSRSEVQEAIDRGEQWLLENLGNVRRATPDAIYNTWAHAYALEAL
                     ARLIQRHKGRPERQAALRELMEGQLDRLIRYECLNGGWAYYDFDYGTQKPGSSTISFV
                     TATVLVALHDAKQVGLTVPPKIIDRAKDSILRQRKPDFSYCYGEYLKMVPMHPVN"
BASE COUNT          253 a          327 c          354 g          279 t
ORIGIN      
        1 tcgatccctg tggcggccga tcggtttgac gtggtggccg aggttacgct tcccaaaaag
       61 ccactgcctt tggtcccggc gatgaccggc acaatccgct tcaatgtcta tgtcaaggag
      121 gacgcgctgg tggttccggc caaagctgtc ttcaccgacg aacttgatcc ggacaaacga
      181 tacgtttacg tactcgttga tgggaagcct gaaaagcgtt ccgtgaaggt gggcgagcaa
      241 gtccgagata aagtggaaat cctcagtgga ctgcgcgatg gcgaggaaat cctattggag
      301 aagcctcaag agtcatccgg cacctagagt tgatgggacg tttttgatga cgattttctc
      361 gcaattctgt ttctccacgt gcgagaccga cgaatgacca cgcgattctc tgttgttggc
      421 gttagctgca tattgctggt gacgtggttc ggccacagtg tcccgcagtt gcatgccgag
      481 gaggtgacgt cctccgtttc acccttagcc acaacgggtc ccaagcccaa acccattgaa
      541 gcggtatcgc cggaggccgt ggaacgagcg atccgccggg gtgtggactt tttgctatta
      601 cggcagaacc gggacggttc ctggggttcg gcacggaata ccaagtcgct caatatctac
      661 gccccggtgc cgggtgctca tcaggctttt cgcgcggccg tgacttcgct ttgcgtttcc
      721 gccctcatcg atgtcaaaga ttctcgatcg gaagttcagg aggcgatcga tcggggcgaa
      781 caatggcttt tggagaatct tggcaatgtt cgccgggcga cacccgatgc tatttacaat
      841 acttgggccc atgcttatgc cctggaggcc ctggcgcggc tcatccagcg gcacaaaggt
      901 cggcccgaac ggcaggcagc cttgcgtgaa ttgatggagg gacaactcga ccggctgata
      961 cgttacgaat gcctcaatgg cggttgggcg tactatgact ttgattacgg cactcaaaag
     1021 ccgggtagtt ccaccatcag ctttgtgacg gccaccgtac tggtggcctt gcacgatgct
     1081 aaacaggttg ggttaaccgt tccaccgaag attattgacc gcgccaaaga ttctattctg
     1141 cggcagcgga agcccgactt tagctactgc tacggcgagt atctcaagat ggtgcccatg
     1201 cacccggtca atc
//
LOCUS       DAOPTR010001183         1210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_89795_length_1210_cov_2.913420, whole genome shotgun sequence.
ACCESSION   DAOPTR010001183 DAOPTR010000000
VERSION     DAOPTR010001183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89795_length_1210_cov_2.913420"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PKI05_16670"
     CDS             <1..397
                     /locus_tag="PKI05_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HOA53889.1"
                     /translation="AKHGGGAFSGKDPTKVDRSAAYMARHIAKNIVAAGLADRCEVQL
                     AYAIGVADPVSIYVDTDGTGKVEERVLCQAIREVFPLTPGGIIEYLDLLRPIYRKTAS
                     GGHFGRNLPEFTWEKTDRISDLLDAVGRA"
BASE COUNT          221 a          329 c          355 g          305 t
ORIGIN      
        1 ggcaaagcat ggcggcgggg ccttcagcgg gaaagaccca acaaaggttg accgcagcgc
       61 cgcatacatg gcccgacaca tcgccaagaa catcgtggcg gcgggcttgg ccgatcgttg
      121 tgaggtccaa ctggcgtacg ccatcggcgt cgccgatccc gtcagcattt atgtggacac
      181 cgatgggacc ggtaaggtcg aagagagagt cttgtgccaa gccattcgtg aggtgttccc
      241 gttgacgccc ggcgggatta tcgagtatct cgatctgcta cgtcccattt atcggaagac
      301 ggcatcgggt ggtcatttcg gtcgaaatct ccctgaattc acatgggaaa agaccgatcg
      361 catctccgat ctgcttgacg cagtgggtcg tgcttagagc ctatcccatg atctcccctc
      421 gcccgttcgg cgggagaggg gtcgggggtg agggtggctc gaatcgttgc cggttttctc
      481 aattgattcc gatgttcggt tgccctcacc ccaaccctct cccggaggga gagggcgact
      541 ttttgagata ggctcttaac cgaataagtt ttcgctggcc gacgccaagg aagttaggcc
      601 ggatttcgcc cattcatggt cagtgtttac cccagcagat ccaactctcg ggaccccatc
      661 gctactgtca cgcgatggct ggtgttggtt ttgggcatct gctgctgggt ggtggccgtt
      721 atcgtggcag tcctcgtttt gacgagggcg gagtcggttg tcttgcgcgc cgtggttctg
      781 cttgtcttcc tccccttggt tctagcgtac ttgattaggg gaagaggagt ggtcgtccaa
      841 cgcatcacac acaggttggt ccaacatccc ttggtcctgg ttgcctttgg cgcggttttc
      901 acggtcggga gcgttctcgc attgttagcc caagagcatt ggtcaggctt cattattacg
      961 gcattgctga taagcagtct gggtgcaatc ctggcaaatt tccccaaatt gcccgaatat
     1021 cacatattac cagacagagg tgataggtct tgccgctgtc catcgggtct tgtcccctcc
     1081 tatgctacta ggcctatttg gctaacaagc gcggtctttc tagagagcct tggattcaag
     1141 ccatcggatt cccttcgcct cggggaactt tcccgcggat ggcttttgtc caccgttcgg
     1201 caagaaggcg
//
LOCUS       DAOPTR010001184         1206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_90250_length_1206_cov_2.943527, whole genome shotgun sequence.
ACCESSION   DAOPTR010001184 DAOPTR010000000
VERSION     DAOPTR010001184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90250_length_1206_cov_2.943527"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="PKI05_16675"
     CDS             complement(<1..826)
                     /locus_tag="PKI05_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53890.1"
                     /translation="MIRHLDMQKDHGNQRTRGFIFSFAFVGIVWFLQAASVIAADWPQ
                     LGGSPSRNAVAITAALPVEWNLGAIDRATWEWKGGPQAQNILWVAPLGTQSYGSPVVA
                     GKYVLCGTNNGRGYLERFPKECDLGCLLCFSRTDGRFVWQYGAEVLPDSNQNWPEQGL
                     CSTPLVEGNRVWVVSNRGCVVCLDLEGFHDGENDGPFAQESSTGPNDADVVWEFDMIN
                     RLGVSPRYMTSSSVTAAGDLLFVTTSHGTNEKGQVVHPEAPSFLALDKHTGELVWAD"
     gene            complement(981..>1206)
                     /locus_tag="PKI05_16680"
     CDS             complement(981..>1206)
                     /locus_tag="PKI05_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53891.1"
                     /translation="LEARPGSYLELNGDTIPVHPGVCVVIPPGVVHRIVGDVVILNIV
                     IPKFDPADEYLAELPSEDCRRCGDHDGPTR"
BASE COUNT          300 a          347 c          311 g          248 t
ORIGIN      
        1 gatcggccca cacaagttcg cctgtatgtt tgtcaagagc aagaaaactg ggggcttccg
       61 ggtgaacgac ctgtcctttt tcgttagtac cgtgggaggt tgtcacaaag aggagatcac
      121 ccgccgctgt gacagatgag gacgtcatgt agcgtggcga cacgccgagg cggttaatca
      181 tgtcgaattc ccacacaacg tcggcgtcgt tcggaccggt actggattcc tgcgcaaagg
      241 ggccatcgtt ctccccgtca tggaaccctt cgaggtcaag acacaccaca caaccccgat
      301 tggacacgac ccacacccga ttgccctcca ctaaaggcgt cgagcaaaga ccctgttcgg
      361 gccaattttg attggaatct ggcaacactt cggctccata ctgccaaaca aagcgaccat
      421 ctgttctgga aaagcacaac aaacagccca ggtcgcattc cttaggaaac cgctcgaggt
      481 atccacgacc gttgttggtg ccgcacaata cgtacttgcc cgccacgact ggcgagccat
      541 aggactgtgt tcccaacggt gcgacccaaa ggatgttttg agcttgaggg ccacctttcc
      601 attcccaggt cgcccgatca attgctccga ggttccattc cacgggcaaa gcagccgtga
      661 tggcaacggc gttacggctt ggagaaccgc ccaattgtgg ccaatcagca gcaatcactg
      721 aagcggcttg aaggaaccac actatcccga caaatgcaaa cgagaaaatg aaacctctgg
      781 tccgttggtt cccatggtct ttttgcatat caagatggcg gatcatgctt agcccccttt
      841 ttgggcaaag atcgacgatg gctgagttac gaaacgtgct ccgaggaacg gtagcgacct
      901 aaccggggac aggccgaaat ccccgatcat tgtgcgagtg ttattatctc cagtgcaagt
      961 gcggttcgac cagactgcca tcaccgggtt ggcccatcat gatcgccaca acggcggcaa
     1021 tcttccgaag gaagttctgc aaggtattcg tccgcgggat caaattttgg aatcacaatg
     1081 ttcagaatga cgacgtcccc gacgatgcgg tgcaccaccc ccggcggaat aacaacacac
     1141 acgcctggat gtacgggaat cgtatcacca ttcaattcca aataggaacc gggccttgcc
     1201 tcgaga
//
LOCUS       DAOPTR010001185         1205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_90360_length_1205_cov_3.014783, whole genome shotgun sequence.
ACCESSION   DAOPTR010001185 DAOPTR010000000
VERSION     DAOPTR010001185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90360_length_1205_cov_3.014783"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PKI05_16685"
     CDS             <1..422
                     /locus_tag="PKI05_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007332043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOA53892.1"
                     /translation="AKDYWTANPVRRYLSVEVFNALLIDRQEIKVHHSPVSDIVSAMG
                     DKYSIIIFPEGGRSSGPEVGPFKSGLYYLAKRRPDLELVPVFLDNMNRILPRGEFLPV
                     PLLTRVIFGPPMWLESGEPKHLFLERARQAILRLKEV"
     gene            611..>1205
                     /locus_tag="PKI05_16690"
     CDS             611..>1205
                     /locus_tag="PKI05_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53893.1"
                     /translation="MTPIKTPRLAIGTIDEETDPWSFLRIFLQELTSQGLHVQTFLSQ
                     ASFFGFQNLKRWSGATPRRLDSWLMSPARCCESFIRGVVGADLAVVVGEYERGGGCHF
                     GTGQHTTDERASFRNVRGPCRFQAEKGSAKVISQGRSRSVCGAPDATSFFGHLDTLCD
                     WLSLSKLVVFRGQGYHEFLGEGPLSEKAMDGVVVVDAD"
BASE COUNT          249 a          300 c          343 g          313 t
ORIGIN      
        1 ctgccaaaga ctactggact gcaaatccag tgcggagata tctctctgta gaggtattca
       61 atgcccttct cattgaccgc caggaaatta aggtacatca ttcgccggta agcgatatcg
      121 tttcggcgat gggcgacaag tattcgatta ttatcttccc tgaaggtggc aggtcgtctg
      181 gccctgaagt tgggcctttt aagagtggcc tctattatct cgccaagcgc cggcccgacc
      241 tcgaactcgt gccggttttc ctcgataaca tgaatcggat tcttccgcga ggggagtttc
      301 ttcctgttcc gctgctcacg cgcgtcatat tcggaccacc aatgtggctt gaatcgggcg
      361 agccaaagca tttgttcctt gaacgcgcac ggcaagcgat tcttcgcctg aaagaggttt
      421 aacccacctt tcgtgcctcc caagaaaacc ccatttttct cacccgacgg ggagaggagc
      481 gggcgaaaag tcgcattcgc tttgtcgtat tgccttgact gtgttgggtt gtccccgaaa
      541 aatgccagca gggagagatc ctattaagtt tagattattt aagcaacttt tacgagtgtg
      601 gccgtagcta atgacgccta ttaaaacacc acgtttggcg atcggaacga ttgacgagga
      661 aacagaccca tggtcattct tgcggatttt cctccaggag ctcacttcac aaggactgca
      721 tgtgcagact tttctgtctc aagcgagttt cttcggattt caaaacctta agcggtggtc
      781 gggggcgaca ccgcggcggc tcgatagctg gctcatgtca ccggcgcgct gttgtgagtc
      841 gttcattcgc ggtgtggttg gcgcggactt ggctgtggtg gtgggggaat acgagcgggg
      901 cggtgggtgt cactttggta ctgggcagca cacgacggat gaaagggcct cgtttcgaaa
      961 tgtgagaggt ccgtgccgtt tccaagcaga gaaaggttcc gccaaggtaa tatcacaagg
     1021 tcggtctcgt tccgtatgcg gcgcgcccga cgccacctca ttttttggcc acctcgacac
     1081 cctttgtgat tggctaagcc tttccaagtt ggtagtcttt cgcgggcaag gttatcatga
     1141 gttccttggc gaaggtcccc ttagtgagaa ggcgatggat ggcgtggtgg ttgtggatgc
     1201 cgatc
//
LOCUS       DAOPTR010001186         1204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_90506_length_1204_cov_2.432550, whole genome shotgun sequence.
ACCESSION   DAOPTR010001186 DAOPTR010000000
VERSION     DAOPTR010001186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90506_length_1204_cov_2.432550"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..785)
                     /locus_tag="PKI05_16695"
     CDS             complement(150..785)
                     /locus_tag="PKI05_16695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOA53894.1"
                     /translation="MAERIVVELRQPGGKRSNRRLRDAGKIPAILYGHKLEPVCLSVP
                     GDALAAAVRHGTRLIQLDGAVKEQAYIKELQWDVWGKEILHVDFTRVSAHERIRSEVP
                     IELRGESPGVKLGGRLAQHLHSIEIECEVDKIPEKIFVNISHLNIGDSITVKDLSVPQ
                     GTKILADPDEIVVVCHPPAGEVEEEAAPEVPGPAEPEVIGRKKAEAEESEE"
BASE COUNT          261 a          368 c          297 g          278 t
ORIGIN      
        1 cacgtactgt cgtccaggat tgccgagccc cacgaccaat ttcatcggag gttgctcctc
       61 ggcttggcct cggcctgaaa acagccatcg ccacatcagt ttccaagcaa cgaccacgcc
      121 gttgccacct ccgactggtc aaagaatact cactcctccg attcttccgc ctcagccttc
      181 tttcggccga tgacctcagg ttcagcaggt cccggtacct ctggtgcggc ttcttcttcc
      241 acctcaccgg cgggcggatg gcagaccacc acgatttcgt ccgggtcggc caagatcttg
      301 gtcccttgcg gaaccgagag gtctttgacg gtgatcgaat cgccgatatt caaatggctg
      361 atgttaacaa agattttttc cggaatcttg tcaacttcgc actcgatctc aatgctgtgg
      421 agatgctgag caagccgccc tcccagcttc acgccggggg attcgccgcg gagctcgatg
      481 gggacctcgc tgcggatgcg ctcgtgcgcc gaaacccgcg tgaaatccac gtggaggatc
      541 tctttccccc agacatccca ttgaagttcc ttaatgtagg cttgctcttt gacagcccca
      601 tctaactgga tcaatcgcgt tccatgccga acggccgcgg cgagggcatc cccgggcaca
      661 ctcaagcaaa cgggttccaa cttgtgacca taaaggattg ctgggatctt ccctgcgtcc
      721 cgtaaacgcc gattgcttcg cttcccgcca ggctgtcgca attccacaac aattcgttcg
      781 gccatctgtg tacccaatgc acaaaagaca aactgtttcc gtatgctgac gcttccgacc
      841 cttcgacacc attaacccga gcgagggtcg tcgaacgttg ccggtcaaga aaaccaccga
      901 cctcgtcacg cgatctgtgt tatgtttgct gccgtgacaa aaccttgatt ttggatgatc
      961 tcccgaaaaa ttcaagggtg gtgggcaaag agatttgtct cctgtcccgc aaagccggcc
     1021 gctctcgcag gtgaccaaat tccctcgctg cgacagcagt ttggagttgc caatagccgt
     1081 gaaaatattg actgtctagc gatacgactt aaaaagtttg ctaacggatt cgtgacggtg
     1141 gatccgcttc acggcctcac ccaacagctc agaaactgtc aaaactttca agttggggag
     1201 gacg
//
LOCUS       DAOPTR010001187         1200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_90913_length_1200_cov_3.629694, whole genome shotgun sequence.
ACCESSION   DAOPTR010001187 DAOPTR010000000
VERSION     DAOPTR010001187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90913_length_1200_cov_3.629694"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..1117
                     /locus_tag="PKI05_16700"
     CDS             119..1117
                     /locus_tag="PKI05_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53895.1"
                     /translation="MPITFQCPACYAMLKVPDDTVGKQAKCPRCGQIVTISAGAPAAP
                     SSDMGAAVPPPIGGEPPRPQEPTSVNPYAPPQAQTSYLAPIDRGTIEPQIISFSETFN
                     VTWRLFFDNIGPFLGMGALLFGLTFVGNMFVNFISLFFAAAGDDEAMIFLGIIVLIFS
                     VLMAAVSMWLQGGMLRYSVELAKGMRPNFGRIFLPVNQFLGYFAVVILLSLIVTAGFI
                     LLILPGIILMLMLFCAPAVYLDGKAGVIESFSVSRTITRGNRLTIFGLVLVNTILAFL
                     ATVLTCGLAGILAPPFFSLLTAVIYTRSAGLFASARPPMVQMPPPSWMPGPSPGLR"
BASE COUNT          248 a          330 c          313 g          309 t
ORIGIN      
        1 tcggaatcga tcgagaaaac cggcagcgac tcgagccacc ctcacccccg acccctctcc
       61 cgccaaacgg gcgaggggag actataggat aggctctaaa tagcagggga acctcaaaat
      121 gcctattacg tttcagtgcc ccgcgtgtta tgcgatgctt aaagtacccg atgacaccgt
      181 tggaaaacag gccaaatgcc cccgctgcgg ccagattgtg acgatttctg ctggtgcgcc
      241 agccgcacca agttctgata tgggcgctgc cgtcccgcca cctatcgggg gagaaccacc
      301 tcgtcctcaa gagcccacaa gcgttaatcc gtatgctcca ccacaggccc aaacttctta
      361 tctggcaccg atagaccgcg ggaccattga gccacaaatt atctctttta gcgaaacatt
      421 caacgtaacg tggcgactgt tcttcgataa catcgggcca tttctcggga tgggggcact
      481 gcttttcggg ctaacttttg tagggaatat gttcgtcaac tttatcagtt tgtttttcgc
      541 ggcagcggga gacgatgaag cgatgatctt tcttggcatc atcgtactca tcttttccgt
      601 gctcatggcc gcggtaagca tgtggttaca aggcggcatg ctccgctaca gcgtcgagct
      661 tgccaaaggg atgaggccca actttgggcg gattttcctc ccagtcaacc aattcctggg
      721 atatttcgca gtcgtaattt tgttgtcgct tatagttact gccggtttta ttctgctgat
      781 cttaccggga attatcctaa tgctcatgct tttttgtgcc ccggccgtct atctggatgg
      841 caaggctggg gtcatcgagt cattttccgt atccagaacc attacccggg gaaatcgtct
      901 tacgattttc ggcctcgtct tggtcaatac gatccttgcc tttctggcga ccgtgctgac
      961 gtgtggactt gccggtattc ttgctcctcc gttcttttcg ttattgacgg cggtcattta
     1021 cacgcgatct gcagggctat tcgcatcagc acgtcccccg atggtccaga tgccgcctcc
     1081 gagttggatg ccggggccga gtccagggct tcgctagcga tttctccaat cgtttcggga
     1141 gaagagcgtg gggaagaaga tcagcgataa cggcaaacag cgcaagagga tgcgctgttg
//
LOCUS       DAOPTR010001188         1198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_91136_length_1198_cov_3.388451, whole genome shotgun sequence.
ACCESSION   DAOPTR010001188 DAOPTR010000000
VERSION     DAOPTR010001188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91136_length_1198_cov_3.388451"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1198
                     /locus_tag="PKI05_16705"
     CDS             <1..>1198
                     /locus_tag="PKI05_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOA53896.1"
                     /translation="EEGSRFFANAVVGNDPWTVQARRNLEIMYHTALAISHTVDIDQL
                     LNRIMDLIFQWVEADRGCIMLIDPETNRLVPKVLRARKGAERPGKLIISQTILDYVRE
                     RNEGVLTSDARHDERWDSGASIFQAGIREAICVPMQGRYGVVGVIYIDTSMSPQEMVR
                     RGNTNKFTEDHLRLMIAIAHQAALAIEDTHYYSAMVQAERLAAIGQTIATLSHHIKNI
                     LQGIRGGSYLIEMGLNKHDEELIRKGWTIVEKNQAKISGLVMDMLTFSKEREPDLQPA
                     DLNQLIADVIELMQSRAEECNVELTWKQEGPIPVLIFDPEGIHRAVLNLVTNAIDATA
                     ERPQPRKVEITTRYDEAQGKVWITVQDNGPGIPPELLDKIFSPFVSVKKGTKGTGLGL
                     PVSQKIV"
BASE COUNT          289 a          371 c          311 g          227 t
ORIGIN      
        1 ggaggaaggt agccgctttt tcgccaacgc cgttgtgggc aacgacccct ggaccgtcca
       61 agcccgtcgc aacctggaaa tcatgtatca cacggccttg gcgatcagtc acaccgttga
      121 tatcgaccag ctcctcaatc gcatcatgga tctgatcttc cagtgggtcg aggccgaccg
      181 cggctgtatc atgctcatcg atcccgaaac gaatcgtttg gtgcccaaag tccttcgggc
      241 acgcaaggga gcagaaaggc ccggcaagtt gatcatcagt caaaccattc tcgactacgt
      301 tcgagaacgc aacgaaggcg tcctcaccag cgacgcgcgc cacgatgaac gttgggattc
      361 cggggccagt atcttccagg caggcatccg cgaggctatt tgcgtcccca tgcagggacg
      421 ttatggagtt gtcggcgtca tctacatcga cacctccatg tctccccagg agatggtccg
      481 ccgcggcaac acgaacaagt ttacggaaga ccatctccgc ctcatgatcg ccattgccca
      541 ccaggcggcc ctggccattg aagataccca ctactattcg gccatggtcc aggcagagcg
      601 actcgccgcc attgggcaga ccatcgccac cctatctcac cacatcaaaa acatcctcca
      661 gggtatccgc gggggcagct acctcattga aatgggcctc aacaagcacg acgaggaact
      721 cattcgcaag ggatggacta tcgtggaaaa aaaccaggcc aagatctccg gcttggtcat
      781 ggatatgctc accttcagta aagaacgcga accggacctt cagccggccg atcttaatca
      841 attgatcgcc gacgtcatcg aactcatgca atcccgggcc gaggagtgca acgttgaatt
      901 gacctggaag caagagggcc ccatcccggt tctcatcttc gatccggaag gcatccaccg
      961 ggcggtgctc aatttagtga ccaacgccat cgacgctacc gccgaacggc ctcagccgcg
     1021 caaggtcgag atcacaactc gttatgacga ggcccaggga aaagtctgga tcaccgttca
     1081 ggacaatggc ccaggaatcc cgcccgagtt gctcgataaa atctttagtc cgttcgtctc
     1141 cgtcaagaag ggcaccaaag gaacgggctt gggcctccct gtcagccaaa agattgtc
//
LOCUS       DAOPTR010001189         1197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_91321_length_1197_cov_1.835377, whole genome shotgun sequence.
ACCESSION   DAOPTR010001189 DAOPTR010000000
VERSION     DAOPTR010001189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91321_length_1197_cov_1.835377"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..1131)
                     /locus_tag="PKI05_16710"
     CDS             complement(106..1131)
                     /locus_tag="PKI05_16710"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOA53897.1"
                     /translation="MSDVLRRRTFVQNAGAAMVGMVASATGGSHVLAANENAKDAETS
                     PASATGFQVGVPEVRLERLRPQEIQDRMNACGTLFLPIGTIEWHGLHNVVGLDAIKAH
                     MLCIRAAQRGGGLVAPPLFGGVGGLNQPHTFVMEPEDQIYSVLLRPWLEKLTMEAARQ
                     GFKAVIILTGHYGAAQQIVVREVGARMTRCLGIPVLATPEYILALDEGYLGDHAAWGE
                     TSLMMYLDPPTVDLSRLGEPPHQGVGGRDPKQFANAEDGRRLAETVIRRLARLSLEMP
                     SWDAASVRAFADAEAALVQRQQELAAQTGKVWTAWRNIGKGVLHRYAECLIERRFDEI
                     KAMAENL"
BASE COUNT          242 a          384 c          313 g          258 t
ORIGIN      
        1 cattcttatt ttggcaaaaa ggaagacact gcttcagagt tctatcctat tcgcttcgcc
       61 ccgtctattg gtagcggttt cccgccccat agttttcgga aaggatcaca gattctccgc
      121 catggccttg atctcgtcga atcgccgttc gattaggcac tcggcataac ggtgaaggac
      181 tccctttccg atattccgcc aagccgtcca gaccttgccc gtctgcgccg ccaactcttg
      241 ttgtcgttgg acgagcgccg cctcagcatc ggcgaaggca cgcacacttg ctgcatccca
      301 ggacggcatc tccagactca gccgggcaag acggcgaata acggtctccg ccagccgtcg
      361 gccatcttcc gcgttcgcaa attgcttggg gtcccggcca ccgactcctt gatgcggcgg
      421 ttcacccaaa cgggataagt ccaccgtcgg cgggtctaaa tacatcatga gcgaggtttc
      481 accccacgcc gcgtgatcac ccaagtaacc ttcgtccagg gcgagaatgt attcgggcgt
      541 agccagcacc gggataccga ggcaacgggt cattctcgcg ccgacctctc gcaccacgat
      601 ctgttgtgcc gcgccgtaat gaccggtcag aatgataacg gctttaaatc cttgtcgggc
      661 ggcctccatc gtaagttttt ccagccatgg ccggagcaaa acggagtaga tttgatcctc
      721 tggttccatg acgaaggtgt gaggttgatt gagacctccc accccaccga aaagtggtgg
      781 cgccaccagg ccgcccccgc gttgcgctgc cctgatgcac aacatgtgtg ccttgatcgc
      841 atccaaaccc accacgttgt gcagcccgtg ccattcgatc gtaccaatcg gtaggaacaa
      901 cgttccgcac gcattcatgc ggtcttgaat ttcctgcggt cggagccttt ccaggcgaac
      961 ctccggcaca ccgacttgga accccgtcgc ggaagccggg gaagtttctg catctttggc
     1021 attctcattt gcggcgagga cgtgggaacc tcccgtggct gaggcaacca tcccgaccat
     1081 ggccgcgccg gcgttttgta caaacgtgcg tcgtcgtaac acatcggaca tcacaccact
     1141 cccgttgcct cggcataagc gaaataacgg taccacccgt tagggcgagt gtcaccc
//
LOCUS       DAOPTR010001190         1194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_91614_length_1194_cov_2.891133, whole genome shotgun sequence.
ACCESSION   DAOPTR010001190 DAOPTR010000000
VERSION     DAOPTR010001190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91614_length_1194_cov_2.891133"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1194
                     /locus_tag="PKI05_16715"
     CDS             <1..>1194
                     /locus_tag="PKI05_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOA53898.1"
                     /translation="KIVDSAERVIRDGASLIILSDRGVNAEWVPIPSLLATSALHHGL
                     LRRGLRGEAGIIVESGEPREVMHFCLLCGYGANAINPYMAFEAIAALHRNKDLPPNFS
                     LDEAIEQYITAVKKGILKTMSKMGISTLRSYHGAQQFEAVGLDRSVVDAYFCGTASRI
                     GGIDLQVIAEEALARHRDAFGPREPAALPLDFGGEYHERVDGERHLWNAETVSKLQHA
                     VINNDWETYLQFAERVNCQNEELCTLRGLFEFEPGEPVPLEEVEPASSIVQRFYTGAM
                     SHGSISREAHEIMAIAMNRLGALSNTGEGGEDPARYRPLPNGDSLNCGIKQVASARFG
                     VTIEYLANAKVLQIKMAQGAKPGEGGQLPGHKVTEEIAKLRHATPGVSLISPPPHHDI
                     YSIEDL"
BASE COUNT          250 a          327 c          368 g          249 t
ORIGIN      
        1 aagattgtgg actcagccga gcgtgtcatt cgtgatgggg cctcgctcat tattcttagc
       61 gatcgcggcg tgaatgcgga atgggtgccg atcccgagcc ttctggcgac ttcggccctg
      121 catcacggat tactccgtcg cggacttcga ggcgaagccg gaattatcgt ggaaagcggc
      181 gaaccccgtg aggtgatgca cttttgcctg ctgtgtggct atggggccaa cgctattaat
      241 ccgtacatgg catttgaggc catcgcagcg cttcaccgca acaaggacct gccgccgaat
      301 ttcagccttg acgaggccat tgagcagtac atcacggcgg tgaaaaaggg aatcctgaag
      361 acgatgtcga aaatggggat ttccaccttg cggagttacc acggggcaca gcagtttgaa
      421 gccgttggcc tggatcgttc tgtggtcgat gcatactttt gcggcactgc ttcgcggatc
      481 ggcgggatcg atcttcaggt tattgccgag gaggccttgg cccgacaccg cgacgccttc
      541 ggtccgcgag aaccggccgc tctgccgctc gattttggcg gggaatacca cgagcgggtt
      601 gacggggaac gccatttgtg gaatgcggag accgtctcaa agcttcagca tgccgtcatc
      661 aacaacgatt gggagaccta tcttcagttt gccgagcgag ttaattgcca aaacgaagag
      721 ctttgcacgc tgcggggact gttcgagttt gagcctggcg agcccgttcc gctggaagag
      781 gtcgaaccgg ccagctcgat cgtccaacgg ttttacaccg gggccatgtc tcatggttcg
      841 attagccggg aagcccacga gattatggca attgctatga accgcttggg cgccctgtcg
      901 aacacgggcg agggagggga ggacccggcc cgttatcgtc cgctgcccaa tggagactcg
      961 ctcaattgcg ggatcaagca ggtggccagt gcccgatttg gtgtgacgat cgagtatctc
     1021 gctaatgcca aggtcctcca gatcaagatg gcccaggggg caaagccagg cgagggcggg
     1081 caattgcccg gtcacaaggt gacagaagaa attgcgaaat tgcggcacgc cacaccgggc
     1141 gtgtccctga tctcgccacc accccatcac gatatctatt ccatcgaaga cctg
//
LOCUS       DAOPTR010001191         1190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_92031_length_1190_cov_3.434361, whole genome shotgun sequence.
ACCESSION   DAOPTR010001191 DAOPTR010000000
VERSION     DAOPTR010001191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92031_length_1190_cov_3.434361"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PKI05_16720"
     CDS             <1..482
                     /locus_tag="PKI05_16720"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HOA53899.1"
                     /translation="EILANPLRITVLAIGLGFLALLVWKVVRILRNPTWSLWLANRLP
                     RRLRDSQLSEHLATAVYRHLEHLLSRQGYRRATGQTPRQFAGSVIQAARADNSACPWA
                     DTVLYAVSLYYRLRFGNGLARPRDWDELGRVVAELDRTFSRKRTVRRAKTAHSDGLK"
     gene            complement(657..1016)
                     /locus_tag="PKI05_16725"
     CDS             complement(657..1016)
                     /locus_tag="PKI05_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HOA53900.1"
                     /translation="MSADDAMGQDVGEIQVRPRRKRRERTDTRHIPRYHVILWNDDDH
                     TYEYVIKMLMQLFGHPFEKAYQMAKEVDLSGRCIVLTTTKEHAELKRDQIHAFGRDPL
                     VAECRGSMTATIEPATE"
BASE COUNT          273 a          335 c          311 g          271 t
ORIGIN      
        1 gagaaatact tgccaatcct ctgagaataa ccgtcctcgc tattgggctt gggtttctcg
       61 ctcttttggt gtggaaagtc gtccggatcc ttcgaaatcc tacttggtca ttatggctag
      121 ccaatcggct acctcgacgg cttagggaca gccagttatc tgaacacctg gccaccgccg
      181 tttacaggca tttggaacat ctcttatcgc ggcagggata ccgacgtgca acaggccaaa
      241 caccacggca gtttgccggg agcgttattc aagccgcccg agcggataat agtgcgtgcc
      301 cctgggcgga cactgtgctc tatgctgtct ccctgtacta tcgactgcga tttggaaatg
      361 gtctcgcgag accgcgcgac tgggatgagc tggggcgagt cgttgccgag ctggaccgaa
      421 ccttttccag gaaacgtacc gtacggaggg caaaaaccgc tcactcggac ggcttaaagt
      481 aacagcctat cctaaaaagt ctccctctcc ctccgggaga gggttggggt gagggcaagc
      541 aaacctcgga atcgattgag aaaaccggca gcgattcgag ccaccctcgc ccccgacccc
      601 tttcccgccg aactcgtgag ggggattatg ggataggccc caagtccatc ccgattttac
      661 tctgtggccg gttcaatggt ggccgtcatc gaaccacgac actcagcgac caacggatcg
      721 cggccgaaag catgaatctg gtcgcgcttc aattccgcat gctcttttgt cgtcgtcaat
      781 acgatgcacc gtcccgaaag atccacttct ttggccatct gataggcctt ttcaaaggga
      841 tgtccaaaaa gctgcatgag catcttgatc acatactcgt aagtgtgatc atcatcattc
      901 caaagaatga cgtgataccg agggatgtga cgggtatcgg tgcgttctct tcgtttccga
      961 cgaggccgaa cctgaatctc gccgacatct tgacccattg cgtcatccgc cgacatagcc
     1021 agcctccagg gtttcaacaa gtcgacgtca ctccagccgg tctaggtttc tccctgacct
     1081 ggctggatag tcggtcaccc caaaagctat tatattgaag ttttccagtt agcgagcagg
     1141 ccaaacggag ttgaggaaaa gccaatatgg ataacgccat agcacgggtg
//
LOCUS       DAOPTR010001192         1187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_92374_length_1187_cov_4.068021, whole genome shotgun sequence.
ACCESSION   DAOPTR010001192 DAOPTR010000000
VERSION     DAOPTR010001192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92374_length_1187_cov_4.068021"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="PKI05_16730"
     CDS             complement(<1..1162)
                     /locus_tag="PKI05_16730"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOA53901.1"
                     /translation="MFRLMIFGAYDRGSLERPRNVQICELRLSGPEGEWPRAPARRPI
                     TNWAEKAGYRPLHFSAPDTTPLLQEAEPIPGEEDVMPDQVIDLTDKLAEDGTLSWQVP
                     EGTWEILRFGYTVSDRAYVSTCSQGWEGYALDVLDAGAFQRYWEAVVEPLLADAGALA
                     GKTLKYLHTDSWEIEPLNWTPTLPEEFHRRRGYAITPWMPVLAGRIVGDRRASHRFLY
                     DFRKTLGDLAIDNHYRLFRDNAHKHGLEIHPESGGPHAVPIDAQRCLGFNDVPMSEFW
                     AWSWRHRIGDANRFFVKQPASAAHTYGRRLVAAEGFTTIGPHWQETLWDNLKPSFDKA
                     CCEGLNLLFWHAFVCSPEEYGLPGLQYFAGTHLNPNVTWWSKSKPFFDYINRC"
BASE COUNT          279 a          354 c          312 g          242 t
ORIGIN      
        1 gacagcggtt gatatagtcg aaaaacggct tggatttcga ccaccatgtc acattgggat
       61 tgaggtgcgt cccagcaaaa tactggaggc ctggcaggcc gtactcctcg ggcgaacaca
      121 caaaggcgtg ccagaacagc aggttaaggc cttcgcagca cgccttgtca aaggaaggtt
      181 tgagattgtc ccacagggtc tcctgccagt gtgggccaat ggtcgtaaac ccctcggcgg
      241 ccaccaagcg gcgaccatat gtgtgcgccg ccgaggccgg ttgcttaacg aaaaaacgat
      301 ttgcatcgcc aatacgatga cgccacgacc acgcccaaaa ctcggacatt ggaacatcat
      361 tgaatcccaa acaccgctgg gcatcgatgg gaaccgcatg gggaccgccc gattctgggt
      421 gaatttccaa cccgtgctta tgggcattgt cccggaacaa ccgatagtgg ttgtcaatag
      481 ccaagtcccc cagtgtcttg cggaagtcgt aaagaaaccg atgacttgca cggcgatcgc
      541 cgacaatccg tccggccaac accggcatcc atggggtgat cgcataaccc cgacgccggt
      601 gaaattcctc aggaagcgtg ggtgtccagt ttaggggttc aatttcccaa ctgtcagtgt
      661 gaaggtactt cagcgtcttg cctgccagcg cccctgcgtc agccagaaga ggttcgacga
      721 ctgcctccca gtaacgttga aatgccccag catcaaggac gtccaaagca tagccttccc
      781 atccttgact acacgtagaa acataggcac gatcgctgac ggtgtaaccg aaccgtaaaa
      841 tttcccacgt cccttccggc acctgccagg aaagcgtccc gtcttccgcc agcttgtcgg
      901 tgagatcaat gacctgatct ggcataacgt cttcttcacc tgggattggt tcggcctctt
      961 gcaaaagggg tgttgtatct ggcgccgaaa aatgtagggg tcggtaaccc gccttctccg
     1021 cccagttagt gatcggacga cgggccggtg cccgcggcca ttccccttcc ggaccactta
     1081 gtcgcaattc gcaaatctgc acgtttcgcg gacgctcaag cgatccccga tcgtatgccc
     1141 cgaagatcat gaggcgaaac atcttgcctt ccgtcaaatc caccaaa
//
LOCUS       DAOPTR010001193         1178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_93424_length_1178_cov_2.528050, whole genome shotgun sequence.
ACCESSION   DAOPTR010001193 DAOPTR010000000
VERSION     DAOPTR010001193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93424_length_1178_cov_2.528050"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..>1178
                     /gene="corA"
                     /locus_tag="PKI05_16735"
     CDS             202..>1178
                     /gene="corA"
                     /locus_tag="PKI05_16735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOA53902.1"
                     /translation="MGAARKSPHHTKRSRRIGRYRIQRRTTPGAAPGTIIPEPTAPSP
                     IITVTRYTADTYEEQVLHDLHQLPQILKKGGTLWCDVDGLGDPAVMSQLAKIFGLHPL
                     AMEDVVNTHQRAKVEEYGDILFIVMRFPHLTDNGVIEAEQISMFLGKDFLLTFQPRPG
                     DAFLPVRKRLHDPAGPLRNAGSDYLAYSLIDLTIDNYFPVLDRLAERLESLDDQIPTS
                     DPRHLLNSIHDLRRELLYLRRTLWPHRDALQSLTRNHHQLLTETTRIHLRDCWDHVMT
                     LIDLTETYRELCADLRDYCMSAISNRMNEVMKWLTLIATVFIPLSFIAGV"
BASE COUNT          274 a          336 c          299 g          269 t
ORIGIN      
        1 gtcaacgacc gggaatgtct ctatgttaca cttacactgc tgtggggtta agtctttcgc
       61 gcggcaaaag gaaagtgcga agtgcggaaa cggtgttcac aaccatgagc caatccgtgg
      121 acaaataaaa gatgttatca tgacctaata agtctcgcag cttttcgaga gttttaacgg
      181 aacgcgagag gaaacacatt catgggtgcg gctcgcaaat ctccccacca taccaagcgc
      241 tcccgtcgca ttggtcgtta ccgaattcag cggcggacga cgccaggggc ggcaccggga
      301 acaatcattc ccgagcccac ggcaccgtca cccatcatta cggtcacgcg atacacggcc
      361 gacacttacg aggagcaggt gctccacgac ctgcatcagt tgccacagat actcaaaaag
      421 ggtggaacgt tgtggtgtga cgtggatggg ctgggcgacc ccgctgtgat gagtcaattg
      481 gccaaaatct ttggtctgca tcccctggcc atggaagacg ttgttaacac gcaccagcgg
      541 gcaaaggtcg aagaatatgg ggacattctc tttatcgtca tgcgttttcc ccatctgacg
      601 gacaacggcg tgatcgaggc cgaacagatt agcatgttcc tcggcaaaga ttttctcctg
      661 acctttcaac caaggccggg cgacgcgttt ctccccgtcc gtaagaggct acacgatccg
      721 gcaggtcccc tgcggaatgc cgggtccgat tatcttgcct attccctcat tgacctcacc
      781 attgacaatt actttccggt cctggatcga ctggcggaac gcctggagtc acttgacgat
      841 caaatcccca cgtccgaccc gcgacatctc ctcaacagta tccacgatct ccgtcgagag
      901 ctgctttatc ttcggcgaac tctctggccg catcgtgacg ctcttcagtc cctgacgcga
      961 aatcatcatc aactcttgac agaaacgaca cgcattcatc tgcgggactg ttgggaccat
     1021 gtcatgacgc tcatcgatct gacggaaacg tatcgtgaat tgtgtgccga tcttcgtgat
     1081 tactgcatgt cggccatcag caaccgcatg aacgaagtca tgaagtggtt gaccctcatt
     1141 gccacggtgt ttatccccct gagctttatt gccggggt
//
LOCUS       DAOPTR010001194         1169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_94465_length_1169_cov_2.866248, whole genome shotgun sequence.
ACCESSION   DAOPTR010001194 DAOPTR010000000
VERSION     DAOPTR010001194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94465_length_1169_cov_2.866248"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /gene="hisG"
                     /locus_tag="PKI05_16740"
     CDS             complement(<1..830)
                     /gene="hisG"
                     /locus_tag="PKI05_16740"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOA53903.1"
                     /translation="MSERILRLGLPAGSLQDATADLFRRAGYKITYVNRSYYPVIDDE
                     EIECVLIRAQEMARYVQDGVLDAGLTGYDWIVETNADVIEVAELVFSKVSRRPVRWVL
                     CVPENSPVQSVKDLEGKRIATEAVGITQRYLEKHGVKAKVEFSWGATEVKPPRLADAI
                     VEVTETGSSLRANNLRIVDEVLTSTPRFIANRESYKDPWKRQKIEDIVLMLRGAMAAE
                     GKVSLMMNVPRAKLQDVIAVLPALQKPTVADLSDPNWVAVITVCDESVVRQIIPRLKA
                     A"
     gene            complement(917..>1169)
                     /locus_tag="PKI05_16745"
     CDS             complement(917..>1169)
                     /locus_tag="PKI05_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOA53904.1"
                     /translation="RAVYFSRSRNRLWRKGEESGHYQIVKEIYIDCDNDAILLKVEQV
                     GGAACHTGYRSCFYRVLTPDGLRPVGQPVFDPEKVYGKK"
BASE COUNT          273 a          354 c          278 g          264 t
ORIGIN      
        1 gcagccttta gcctggggat aatctggcgg acgacggatt cgtcgcacac cgtaatcaca
       61 gccacccagt tagggtcgga gagatcggcc acagtgggct tttgcaaggc cggcaagaca
      121 gcgatgacat cttgtagctt cgctcgcgga acgttcatca tgaggctgac cttaccttcg
      181 gccgccatgg ccccgcgaag catgagaacg atatcctcga tcttctgccg tttccacggg
      241 tctttatagg actcccgatt agcaataaat cgcggggtgc tcgtcaagac ctcatccacg
      301 atccgcagat tattcgctcg caaagagctt cccgtttccg tgacttccac gatggcatcg
      361 gccaagcggg gcggtttgac ctctgtagca ccccaactaa actccacctt ggcctttacg
      421 ccatgtttct ccaaatagcg ctgggtaatt cccacggcct cggtggcgat acgtttgcct
      481 tccaggtctt taaccgactg gacgggcgaa ttctccggca cacacaacac ccagcggact
      541 gggcgacggc tcactttcga gaagacaagt tccgcaactt cgatcacgtc cgcgttcgtc
      601 tcaacaatcc aatcgtaccc ggtcaaaccg gcatccagaa caccatcttg gacgtagcgc
      661 gccatctcct gagcgcgaat caggacgcac tcgatctcct catcatcgat cacgggatag
      721 taactgcggt tgacataagt gattttgtaa ccggcacgac ggaacaagtc ggcggtggcg
      781 tcttgaagac ttcccgccgg caaaccaaga cgcaagattc tttctgacat tcctaatctg
      841 acctcgtaac tacgaaactc aattttggta acccaccaag aagttattcg attcccagca
      901 gggtacgatg atcgtcctac tttttcccat acactttttc cggatcaaac acgggctgac
      961 cgacaggcct aagcccatcc ggcgtgagaa cacggtagaa acaggagcgg taacccgtgt
     1021 gacaggccgc ccctcccacc tgttcaactt tcaaaaggat cgcgtcattg tcgcagtcga
     1081 tgtagatttc cttgacaatc tgataatggc cactttcttc tcccttccgc cagaggcgat
     1141 ttcgactgcg actaaaatat acggcccga
//
LOCUS       DAOPTR010001195         1167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_94665_length_1167_cov_4.583633, whole genome shotgun sequence.
ACCESSION   DAOPTR010001195 DAOPTR010000000
VERSION     DAOPTR010001195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94665_length_1167_cov_4.583633"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..498)
                     /locus_tag="PKI05_16750"
     CDS             complement(133..498)
                     /locus_tag="PKI05_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOA53905.1"
                     /translation="MSARVLVADDSSTMRKIIIRSLQAVGVQDAVEAADGNEALALFQ
                     PGAFNLVLTDWNMPGKSGLEVVQGIRAQDPNVPIIMVTTEAEKSRVLQAIQAGVSDYL
                     VKPFTADTLREKLEKHGCG"
     gene            complement(744..>1167)
                     /locus_tag="PKI05_16755"
     CDS             complement(744..>1167)
                     /locus_tag="PKI05_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOA53906.1"
                     /translation="EEVEGIEAMPILVQRAPRYVYVLGEVESPGRFELTGPTTVIQAI
                     SMAGGWKIGANLRQVVIFRRGDDWRLLATMLDLQAALHGNKATPCDEIWLSDSDVVIV
                     PKSFLQRSDDFIEMVFTRGIYGVLPLQTTINFSKLSTI"
BASE COUNT          260 a          360 c          306 g          241 t
ORIGIN      
        1 gacgagaaca tcctgaattc cacgcgctcc accgacttcc tgacgcccaa gaccgccgaa
       61 ccgataggcg agaaacccaa gctcgacgtt ccgcttttcc acagactcca gtgatcaagt
      121 ccggcccggc cattacccgc aaccgtgctt ttccagcttc tcacggaggg tatccgccgt
      181 gaagggcttg acgagatagt cggaaacacc cgcctgaatg gcctgtaaga cgcggctttt
      241 ctcggcctct gtggtcacca tgataatggg gacattcgga tcttgggcgc gaatcccttg
      301 gaccacttcc agcccggatt tccccggcat attccaatcc gtcaacacga ggttaaaagc
      361 ccctggctgg aacaaggcca aggcctcgtt tccgtcggcg gcctccacgg catcctgcac
      421 gcccacggcc tgaagcgagc gaatgataat tttgcgcatg gtgctagagt catcggcaac
      481 aagaactcgg gcgctcatcg ctcctccctt gtgtcattgt caaaagatgc gttcgatcga
      541 agatggtatg acaagtttcc cgaaaagtgg gaattcccta tttggccaat ttgggctagg
      601 aatccccggc gcgcttactc cacgtgaacc gcctctgaca agacgtgcac ggcggttcgg
      661 tttgtaaaaa cgtaggtcac gcaactgcgc ctgtcaagaa aacgacaaac cgaccgaaga
      721 acgcccccgt gggtggtatc cgctcaaatc gtgctgagct ttgagaagtt aatcgtcgtt
      781 tgcagcggta ggacgccgta aatcccgcgg gtgaacacca tctcgatgaa atcgtcgctc
      841 cgttggagga agcttttggg gacaatgacc acatccgaat cagacagcca gatttcatcg
      901 cacggggttg ccttattgcc gtgcagggcc gcctggaggt cgagcatcgt agccaacaat
      961 cgccagtcat ccccgcggcg gaagatcacc acttgccgca gattggcccc tattttccat
     1021 ccgccggcca tactgatggc ctggatgacc gtggttgggc cggtcaactc aaaccggcca
     1081 ggggactcca cttctcccaa aacgtaaacg tatcgcgggg cccgctgcac caggatcggc
     1141 atggcttcaa tcccctcgac ttcttcc
//
LOCUS       DAOPTR010001196         1167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_94675_length_1167_cov_3.866007, whole genome shotgun sequence.
ACCESSION   DAOPTR010001196 DAOPTR010000000
VERSION     DAOPTR010001196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94675_length_1167_cov_3.866007"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="PKI05_16760"
     CDS             complement(<1..907)
                     /locus_tag="PKI05_16760"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOA53907.1"
                     /translation="MANCYLVTGCAGFIGSRVCEMLLADGHEVIGWDELNDAYDPRLK
                     QSRLERLQQHSRFWFSQIDVADQQAVERAFPKQIVAVVNLAARAGVRASVANPQVYYR
                     ANCLGTLVLLEMCRRYGVKKFLLASTSSLYGAHNPVPYREDTDTNRPLSPYAASKKAA
                     ETLAYTYHYLHGIDVSVVRYFTVYGPAGRPDMSIFRFIRQIAEGEPIVVFGDGTQRRD
                     FTYVDDIARGTIAALRPLGYEIINLGGDRSVELNMVIAEIARLLNKQPTVVHQPPHPA
                     DVPATWADVTKARTILGWNPQVPIEE"
BASE COUNT          286 a          331 c          295 g          255 t
ORIGIN      
        1 cctcttcaat cgggacttgc ggattccaac ccaggatcgt ccgggctttg gtgacatcag
       61 cccacgtggc gggaacatcg gccggatggg gtggctgatg gaccaccgtg ggttgcttgt
      121 tgagcagtcg tgcgatttcg gcaatcacca tattcaactc cacggaacga tctcccccga
      181 ggttgatgat ttcgtaaccc agggggcgaa gggcggctat ggtacctcga gcgatatcat
      241 ccacatacgt aaaatcgcgc cgttgggtcc catccccgaa gacaacgatt ggttcaccct
      301 cggcgatctg gcgaataaag cggaagatgc tcatatccgg ccgcccagca ggcccgtaaa
      361 ccgtgaagta ccggacaaca ctcacgtcaa tcccatgaag atagtgataa gtgtaagcca
      421 aagtctcagc cgcctttttg gatgcggcat atggggacag cgggcgattg gtgtcagtgt
      481 cctctcgata agggacagga ttatgcgccc cataaaggct tgaggtggaa gccaggagaa
      541 atttcttgac cccataccgg cggcacattt ctaacaggac gagtgtcccc aggcaattgg
      601 ctcggtaata aacctggggg ttagctacgc ttgctcttac gcccgcccga gctgccaggt
      661 tgaccacggc aacaatttgc ttcggaaaag cgcgttcaac cgcctgctga tcggccacat
      721 caatttgcga gaaccaaaac cgcgaatgtt gctgcaacct ctccagtcgc gattgcttga
      781 gccgcgggtc gtaagcatca ttcagttcat cccacccaat gacctcgtga ccatcagcca
      841 aaagcatctc gcagacccgc gatccaataa agcctgcaca gcctgtcacc agatagcaat
      901 ttgccataat tctccctctc acgccgacgt cgcacgcttc caacttgcgt taccctaggt
      961 atggttttcg aaagactcca ttctaaacga gaaacccaat cagagtaatg gcacaataag
     1021 ggcaaagtgt ggctatcatt ggtcccgcaa aagagtattt ttgtaacggg gcgctgactg
     1081 ttttcttcaa accctccacc ttctcccact gcgctctggt atgatcaaag caaggaacgc
     1141 gataaaatag gcttgctact gtcggac
//
LOCUS       DAOPTR010001197         1161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_95423_length_1161_cov_2.509042, whole genome shotgun sequence.
ACCESSION   DAOPTR010001197 DAOPTR010000000
VERSION     DAOPTR010001197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95423_length_1161_cov_2.509042"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PKI05_16765"
     CDS             complement(<1..430)
                     /locus_tag="PKI05_16765"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOA53908.1"
                     /translation="MLRYWTAGESHGKGILALVDGFPAGVELNLEIINRELLRRQGGF
                     GRGGRQRIEQDQAEILSGWWRGVTIGSPIAIWVANRDYKIEKMDDLTRPRPGHGDLAG
                     AVKYFSSIRGILERASARETVGRVAAGALARQLLSACQIDL"
     gene            895..>1161
                     /locus_tag="PKI05_16770"
     CDS             895..>1161
                     /locus_tag="PKI05_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53909.1"
                     /translation="MKAIQAARLANVVRHVFYWGCFSIFMTSIISRVGISLAAQPAIG
                     QIETLPGTEPLDWSGDIASRMIDSIDAFLLRQTEHTAKVREQAWA"
BASE COUNT          250 a          317 c          313 g          281 t
ORIGIN      
        1 aaaggtcaat ctgacaagca ctaagtaatt gccgggccaa agcccccgcg gccacacgtc
       61 caactgtttc ccgtgcactt gcccgctcga gaatcccgcg aatggatgag aaatacttga
      121 cagcgccggc caagtctcca tgcccgggac ggggtcgtgt aaggtcgtcc attttctcga
      181 ttttgtaatc gcgattagcc acccagatag caatggggct tccaatcgtg acccctcgcc
      241 accagccact gaggatttct gcttggtctt gttcgattcg ttgtcgtccc ccgcgaccga
      301 atccaccttg tcgccgaagt aattcgcgat tgatgatctc cagattgagt tctaccccgg
      361 cgggaaaccc atcaacaagg gcaagaatcc ccttaccatg tgattcaccg gctgtccaat
      421 accgtagcat aactcttttt gtcctcgata tttgggcaat tcgcgttgag ctttgagttc
      481 aaatgtactg tgattgcaag catttctcta ttggattccc gccattgtat cgccctacgc
      541 aaggttatcc cagagcgaat aagtgttgcc ctgccgcacg ggaggcgacg gcagaggttc
      601 cttcagcgtt tttgttttgg tgggccgtgc gccaccgcga tcagaacgaa tgattcgcgc
      661 gaccgtcgtg cagggcttta ccgcacaaat cagtggcagt cacaacggaa gtgccgcatg
      721 cagttgggac aaatttgagc gcttcgttac gacagcacac ctttcgtgct cgggcatatg
      781 gttgagcctt acgggttctc gtacactggt tagcggcaga aatcggatgg tgtgttggtc
      841 atgggaggcc aacgtcctgt tcgaatgaag actggactga gcggaggtct gatcatgaaa
      901 gctattcagg cagcgaggct ggcaaatgtc gttaggcacg tgttttattg gggatgtttt
      961 tccatcttca tgaccagtat tatcagccgc gtcggtatct ctttggccgc tcagccggca
     1021 atcggccaga ttgagacgct gccaggcacc gagcctctcg actggtcggg ggacatagcc
     1081 tcgcggatga tcgactcgat cgacgcattt ctccttcgcc agacagagca caccgccaaa
     1141 gtgcgagaac aagcgtgggc a
//
LOCUS       DAOPTR010001198         1160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_95540_length_1160_cov_2.698643, whole genome shotgun sequence.
ACCESSION   DAOPTR010001198 DAOPTR010000000
VERSION     DAOPTR010001198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95540_length_1160_cov_2.698643"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1160
                     /locus_tag="PKI05_16775"
     CDS             <1..>1160
                     /locus_tag="PKI05_16775"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOA53910.1"
                     /translation="PHKVQDTQFTAKLINGRVIQPQRELPLLAQCEVLVVGGGPAGVS
                     AAIAARRAGAHVVLVERYGHFGGLWTGGLVLLVLGHIVTGGKQVCMGIGEEMMQRLEK
                     LPGGIINRQPGKNPTVDAEALKYVMVEMIEEAGVEVFLHAWGVDAILEGNKVRGAVFE
                     SKSGRQAILADVVVDATGDGDIFAAAGAEFEHRTHNVGLVSRVGNLDRVDPSRVPPEK
                     RPKRLGGPTPAPGINWVNLTGPEVDGLDVRTLTRMEMSHRKFIWRNVQQARQVPGYES
                     LFLVETAPQLGVRITRVLNGLATVRYADLKAGRRFEDVVAVGGSTNGQHGEWQVPYGA
                     LVPKTVDNVLAAGRCISAEMKMADLVRLIPNCFATGHAAGVDVDPAHTGREG"
BASE COUNT          230 a          302 c          390 g          238 t
ORIGIN      
        1 cccgcacaag gttcaggaca cgcaattcac cgcaaagctg atcaatggac gcgtcattca
       61 accacaacgg gaattacctc tgctagctca gtgtgaggta cttgtcgtcg gcggtggacc
      121 ggccggggtc tcggctgcta tcgcggcgcg ccgtgcgggt gcccatgtcg tcctggtgga
      181 acgctacgga catttcggcg gcctgtggac cgggggattg gtccttctcg tcttgggaca
      241 tattgtcaca ggcgggaagc aagtatgcat gggtattggc gaggagatga tgcagcgcct
      301 ggaaaaattg ccgggaggta ttatcaatcg tcagcccggg aagaacccca ccgttgatgc
      361 cgaggcactg aagtacgtga tggtcgagat gatcgaggag gcgggcgtcg aagtcttttt
      421 gcacgcatgg ggtgtcgatg ccattttgga agggaataaa gtccgtgggg ctgtctttga
      481 aagcaagtcg ggacgacagg ccattcttgc cgacgtcgtg gtagatgcga cgggcgacgg
      541 ggatatcttc gccgcggcag gggcggagtt cgaacaccgt acgcacaacg tggggctggt
      601 gtcgagggtg ggcaatctcg atcgagtcga tccctcgcgg gtcccgccgg aaaagcgtcc
      661 caaacgattg ggcggtccta cgcccgctcc aggaatcaat tgggtcaatc taacagggcc
      721 ggaagtggat gggcttgacg tccgcaccct gacccgcatg gaaatgtctc accgaaagtt
      781 catctggcgg aatgtgcaac aggcccggca agtgcccggt tatgagtcgc tctttttggt
      841 cgaaaccgcc ccgcaattgg gtgttcggat cacccgggtc ttgaacggtc tggccaccgt
      901 tcgctatgcc gatttgaagg ccggtcggcg tttcgaggat gtcgtagcgg ttggtggctc
      961 aacaaatggt cagcacggcg aatggcaggt gccgtatggt gctttggttc ccaaaacggt
     1021 ggacaatgtc ctggcagccg ggcgatgcat cagtgccgag atgaaaatgg ccgatctggt
     1081 gcggttgatc cccaactgtt ttgccacggg ccacgccgcg ggagtcgacg tggacccagc
     1141 ccacaccggg cgtgaaggtc
//
LOCUS       DAOPTR010001199         1158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_95801_length_1158_cov_2.330916, whole genome shotgun sequence.
ACCESSION   DAOPTR010001199 DAOPTR010000000
VERSION     DAOPTR010001199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95801_length_1158_cov_2.330916"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PKI05_16780"
     CDS             <1..297
                     /locus_tag="PKI05_16780"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOA53911.1"
                     /translation="GWASGTRATLRNTGTPPANWLRLRAQLDQSTKDQDEKTQPETTP
                     QSETVSKVGGFSSAHPGVVQALMGNGTVRPVVWTIDQTVWKQMAHRCDGSLPGG"
     gene            344..790
                     /locus_tag="PKI05_16785"
     CDS             344..790
                     /locus_tag="PKI05_16785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53912.1"
                     /translation="MLPDSENPFAPPQATSTVSPLGDQLTWSLGIREIVVAVVVLLVL
                     GGGVATSYALIENSVIPIEKGMLAEAERDLGGMMVASLLTAIFVLGIVLWNEGILFSA
                     GGLIALLLGTVIWGVATTVLFTPLGAIVIWVTVLLAVILGAVLLRR"
BASE COUNT          235 a          278 c          357 g          288 t
ORIGIN      
        1 ggttgggcct cgggaacaag ggccacattg aggaataccg gcacaccgcc ggcgaactgg
       61 ttgcggcttc gtgcgcaact cgatcaatca acgaaggatc aagacgaaaa gacacaaccg
      121 gaaacaacac cgcaaagtga gacggtgagc aaggtcggcg gtttttcgtc agctcatccc
      181 ggcgtggtac aggccctgat ggggaatgga accgtccggc ccgtggtttg gacgattgat
      241 caaacggttt ggaagcagat ggcccatcgg tgcgatggtt cgctgccggg gggttagttt
      301 ccccatgaat gtcacgtctt gaaagatttg gtggactcag ccgatgctgc ccgattctga
      361 gaaccctttt gccccgccgc aggcgacaag cacggtctcg ccgcttggcg accaattgac
      421 ctggagccta ggcattcggg aaattgtggt tgcggtggtc gtcctcttgg tccttggcgg
      481 aggtgtggca acatcatatg ccttgattga aaactctgtt atcccgatcg aaaaggggat
      541 gttggcagaa gcggaaagag atttgggcgg aatgatggtt gcctctttgc tgacggcgat
      601 ctttgtgctg ggcatcgtgc tttggaacga ggggatcctc ttctcggcgg ggggccttat
      661 tgcgcttttg cttggcaccg tgatttgggg ggtggccacc acggtcctgt tcacgccgct
      721 cggtgcgatc gttatttggg tcacggtgct gctggcagtg atcctcggag ctgtgctcct
      781 cagaaggtaa ctccggcaga atgtgttaga acatgtcttc tacgtgccgg atggcttagg
      841 aataaaccga caaaaaggta atgatgcgtg ccaatcctcg atgagtgggc aaccgcagct
      901 atgtctcttt ctgttctatg aagttgggtc aaaccatgta cctgcccgat ggcatggcgg
      961 gaggggacat ctttttcgct cttgccgatt aatcgggcaa tttgccattt cgggggaata
     1021 ccgaagactc ctgtgttttc tgagtcaaga tttgctcaat cccctggggt gttctaggct
     1081 attataggaa tcctttacgc acaagggact ttcccccgtc gggaatcatc ttcgggggag
     1141 tcggggatta tctatctg
//
LOCUS       DAOPTR010001200         1157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_95912_length_1157_cov_2.714156, whole genome shotgun sequence.
ACCESSION   DAOPTR010001200 DAOPTR010000000
VERSION     DAOPTR010001200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95912_length_1157_cov_2.714156"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PKI05_16790"
     CDS             complement(<1..272)
                     /locus_tag="PKI05_16790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA53913.1"
                     /translation="MKRRDFLKLGSAGVIMGTMPGYAVGLTRTKPRRIGLIGCGWYGK
                     SVLLRFLQVEPCEVVALADVDGQMLEEAANIVAERQSSHKKPKTYR"
     gene            716..>1157
                     /gene="folE"
                     /locus_tag="PKI05_16795"
     CDS             716..>1157
                     /gene="folE"
                     /locus_tag="PKI05_16795"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007333587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HOA53914.1"
                     /translation="MLHPKDKSVPVFPVDVQRIEHAVREILLAVGEDPDREGLRETPA
                     RVARMYAELFQGLRADPRRHLKKVFVEKYDEVVLVKDISFESICEHHLLPFMGKAHIA
                     YLPDGKVVGLSKLARVVEDISRRPQVQERMTEDIADLLMEELNAK"
BASE COUNT          295 a          333 c          285 g          244 t
ORIGIN      
        1 tcccgatacg tcttcggctt cttgtgcgaa ctttgccgct ccgccacgat attggccgcc
       61 tcctcgagca tttgcccatc gacgtccgca agggccacga cctcacacgg ctcgacctgg
      121 aggaatcgca gcaaaacact ttttccatac cagccgcagc cgatcaagcc aatgcgccgg
      181 ggcttcgtcc gcgtcaagcc gacagcgtaa cccggcatcg tccccatgat cacacccgcc
      241 gatcccaatt tcaggaagtc ccgtcgtttc atgatgactc tcctatcttc actcgattta
      301 cagagctccc tcaaaacttg accgacaacg gccgagacaa ctctggccac acaaacccaa
      361 caccttcccc gctgggtccc cacgggcttt ggatattatg cctaatgcct tacccaaaat
      421 aaaccacgcc attaaaacat cttcctggtc acgcccatac gacataactc cggaggtccc
      481 aaccaatacc acattggcct gtttactttc cggcaagcga gaaaaagacg ctggagaaaa
      541 ccacgctgcc aaccccaaaa tgaggacaga taccaccaca cttaggcctg ttgtcggcgc
      601 gattcacgta taatttacaa gttggcagtg acattggcga tgaatcggat gaggtagaac
      661 aatgacaatg gctgactcaa tgaaccagga ggtgcccagc gagaacagcg gcgagatgct
      721 gcatcccaag gacaagtccg tgcctgtgtt tcccgttgat gtacaaagaa tcgaacacgc
      781 agtgcgagag attcttcttg ccgtggggga agatccggac cgcgaagggt tgcgagaaac
      841 tcccgccagg gttgccagga tgtacgccga gctttttcag ggtttaaggg ccgacccacg
      901 gcgtcatctc aaaaaggtct tcgtagaaaa gtatgatgag gtggtcctgg tcaaggatat
      961 tagcttcgaa agcatttgcg agcaccattt gcttcctttc atggggaagg cccatatcgc
     1021 ctatcttcct gatggaaaag ttgttgggtt aagcaaactg gcgcgggtag tcgaggatat
     1081 ctcgcgtcgc cctcaagttc aggagagaat gacggaagac attgccgacc tattgatgga
     1141 ggaactcaac gctaagg
//
LOCUS       DAOPTR010001201         1152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_96513_length_1152_cov_2.736554, whole genome shotgun sequence.
ACCESSION   DAOPTR010001201 DAOPTR010000000
VERSION     DAOPTR010001201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96513_length_1152_cov_2.736554"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="PKI05_16800"
     CDS             complement(<1..1101)
                     /locus_tag="PKI05_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53915.1"
                     /translation="MGHVNWKEWFLVHTRLHPRDVEALKDGGFVEGIEIAGGTNEEDR
                     ALYFDNLTPYLEPLPPLTFQPRPRRPLTLPEGQPTGVHTGPGVLPFPTREETILPDQL
                     TPNYQIEVLQNGEAYEFRYRGDDGQLVYRYTPQHGHWGDITVTWNNALSFRPMVSGGI
                     RLRTAEGRLVEPQYKLMSCAVEGDAVVARWQVEAANCHGEVTYSFRLWQKSLVIDVAC
                     LGGIVGEVSFGYAEGLPHPRLVTLPYLTCEAQRPAVVVAGDEKAPLFMMGLVDYYRSN
                     ASALFAINDSREDHLTYNGGARYLPKTDGKLSDAFERLFLTVSPRFEEVLPNIPNPRS
                     PWMHVTGERVWRAHGASNREKDYQLWKRVARYG"
BASE COUNT          246 a          348 c          325 g          233 t
ORIGIN      
        1 gccatatcgg gcaacgcgtt tccacaactg gtagtctttt tcacgatttg atgccccatg
       61 ggcccgccat acccgctcgc cggtgacatg catccacggt gaccggggat tagggatatt
      121 ggggaggacc tcctcgaagc gcggcgatac cgtaagaaac aaccgctcaa aggcatcgct
      181 gagtttgcca tcggtcttgg ggagatagcg ggcaccgccg ttatatgtca ggtgatcttc
      241 cctggaatcg ttaatcgcaa acagggcaga cgcattagag cggtaataat cgaccagacc
      301 catcataaag agtggcgcct tttcatcacc ggcgaccacc acggcagggc gttgggcctc
      361 gcatgtcaag taaggcagcg tcactaaccg cggatggggc aggccctcgg cataaccaaa
      421 cgacacttcg cccacaatcc cgccaagaca ggccacgtcg atgacaaggc ttttttgcca
      481 caatcgaaag ctgtaggtca cctcaccgtg gcaattcgct gcttccacct gccaccgagc
      541 aaccacggcg tccccttcca ccgcgcagct catcagcttg tattgcggtt cgaccaggcg
      601 gccctcggct gtgcgaagac gaatcccgcc gcttaccatg ggtcgaaaac ttagtgcgtt
      661 gttccatgtc acggtgatgt caccccaatg accgtgctgg ggtgtgtaac gatacacaag
      721 ctggccgtcg tccccgcgat accgaaactc atacgcctcg ccattttgga gcacttcgat
      781 ttgataattc ggcgtgagct gatcggggag gatcgtttcc tctcgcgtgg ggaagggcaa
      841 aacgccaggg cccgtgtgaa cccctgtcgg ctgaccctca ggcaatgtca gtgggcgacg
      901 cggtcggggc tgaaaggtca acggcggcaa cggctccaag tacggtgtga gattgtcgaa
      961 gtagagcgcg cggtcctcct cattcgttcc cccagcgatt tcgatcccct ccacaaagcc
     1021 gccgtccttc agggcttcca cgtcccgggg atgaagccgg gtatggacga gaaaccattc
     1081 cttccagtta acgtggccca tttcggcgcg atcagggtgt ccttcgcgtg accttaagag
     1141 aacggtgaca cg
//
LOCUS       DAOPTR010001202         1150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_96760_length_1150_cov_5.343379, whole genome shotgun sequence.
ACCESSION   DAOPTR010001202 DAOPTR010000000
VERSION     DAOPTR010001202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96760_length_1150_cov_5.343379"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            350..>1150
                     /locus_tag="PKI05_16805"
     CDS             350..>1150
                     /locus_tag="PKI05_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOA53916.1"
                     /translation="MTYEVTLITGDGVGPELAAAARRCVDATGVRINWDVQEAGVDVM
                     ERLGTPLPEATLASIRRTRCALKAPITTPVGTGFRSINVFLRQELGLYACVRPCKYYP
                     GVRTFFQNVPVDLVIIRENTEDLYAGVEFERGQPATSQLIDCINQLSTDRKIKTPAAE
                     TGISIKPISVSGTERIVRYAFRYARENGRKKVTAVHKANIMKFTDGLFLEVARQVATE
                     YPDIEFEDRIVDNMCMQLVQKPELYDVLVLPNLYGDIISDLAAGLVGGL"
BASE COUNT          248 a          320 c          313 g          269 t
ORIGIN      
        1 cgtttgcgat gcacccaata ttgaggatgc ctgatcccct tcctaactcc ccaagatgat
       61 gtaggttatc attttactcg atgcccccat gggtctccag caccaccaaa actaccccct
      121 taggtcccgt tggcctctgc gggtgaccac ctcggttttc attacggccc agataaaaac
      181 gaaatacggt atcgtttcta gttgccgcgg cgcccgagtg tgtttcaccc gtcaccccga
      241 cgtggaccgt gtggacgaga ccttgtttct ccggtaggct attaagtctc aggctgtcct
      301 ttcgcggagt tgcaaggtta caaaacggga gcttgctgag ggtaacttca tgacatatga
      361 agtcactttg attacaggcg atggcgtggg tccggaattg gcggctgccg ctcggcggtg
      421 cgtggacgcg acgggcgtcc ggattaactg ggatgtccag gaggccgggg ttgacgtcat
      481 ggaacggcta gggacccccc ttccggaggc cacattggcg agtatccgcc gcacccgttg
      541 tgccctaaag gcccctatca ctacgcccgt tggtaccggc ttccgcagta ttaatgtctt
      601 cctccggcag gagctggggc tttatgcctg tgttcgcccc tgcaagtatt accccggtgt
      661 gaggacattc tttcagaacg tgccggtcga tcttgtcatt atccgcgaga acaccgagga
      721 cctttacgcg ggcgtcgagt ttgaacgcgg acaacccgca accagccagt tgattgactg
      781 tatcaaccag ttatcgacag accgaaagat caagacaccg gccgcagaga cgggcatttc
      841 catcaagccg attagtgtct cgggaacaga gcgaatcgtt cgctacgctt tccgctatgc
      901 ccgggaaaat ggccgaaaaa aggtcactgc cgtgcacaag gcgaatatca tgaaattcac
      961 tgacggcctt ttccttgaag tggcacggca agtggcaaca gagtatcccg acatcgaatt
     1021 cgaagaccga atcgtcgata acatgtgcat gcaattggtc cagaagcccg agttgtatga
     1081 cgtccttgtc ctaccgaatc tctacggtga catcattagc gacttggccg ccggacttgt
     1141 tggtggtttg
//
LOCUS       DAOPTR010001203         1149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_96891_length_1149_cov_3.968007, whole genome shotgun sequence.
ACCESSION   DAOPTR010001203 DAOPTR010000000
VERSION     DAOPTR010001203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96891_length_1149_cov_3.968007"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PKI05_16810"
     CDS             complement(<1..425)
                     /locus_tag="PKI05_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53917.1"
                     /translation="MRKYTVFLALVWSLLIVGLVGCPKPQAPEKPPAEGDLQAKETVQ
                     VNPADVEALVKRVEQLGGKIEYDQNKNPVAVDLFQRQATDEDLALIAKVPTIRRVSLW
                     GAEIHDTGIQELTKLPQLEWLVLENTNITNDGVKTLGQI"
     gene            858..>1149
                     /locus_tag="PKI05_16815"
     CDS             858..>1149
                     /locus_tag="PKI05_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53918.1"
                     /translation="MCQQHQCSTCFNRRDCLKMVSLAAMGTGFLASLGQEPSANEGPG
                     LTDYVDVKTLRPKPDVRVAATFLELPRPYWLGWPGTTYDLDGHQKEYTALLNA"
BASE COUNT          232 a          322 c          310 g          285 t
ORIGIN      
        1 ttgatttgcc cgagagtctt gacaccgtca ttcgtgatgt ttgtattctc taacacaagc
       61 cattcgagtt gcggaagctt ggtcagctcc tgaattccgg tgtcatgaat ctcggccccc
      121 cacaggctga cccttcgaat ggtaggaacc tttgcgatga gggccaaatc ctcatcggtt
      181 gcttgccgct ggaagagatc aaccgcaacc gggttcttgt tttggtcata ctcaatcttg
      241 ccgccgagtt gctccacacg cttgacgagc gcttccacgt cggccggatt aacttgcacg
      301 gtctccttgg cctggagatc gccttcggcg ggtggctttt cgggcgcctg gggtttaggg
      361 catccgacca aaccaacgat cagcaggctc catacgagag ccaagaaaac ggtgtacttc
      421 cgcatgaatc acctctctta gaaacgcacg tctcaatgct cgaccgttat cgctcgattg
      481 tgtccacgct tgcgctggcg atgtcccaaa ccttttacgt tatcatgagg cccagaagtt
      541 cgcaaacggg tcacccgcgg tgaatttgta aaggtttttc tgagcgattt gcagggacac
      601 acgctcaggt tgcactcaat ctgctaagga cgcgctctcg cttctgccgt tgtgccaatc
      661 gatcattcat ggttgaccca gccacgatgg cgcgtggccc cccaacacaa tatgcttcat
      721 gcccattgtt gcagatagcc tcgggaaagg ttactattct gtcggcggca gattggggaa
      781 tcttcggccg cctcgttttc ggtaacagtt cggcgacttt tcacgcatcg ttcggtggtt
      841 gactgggagg acgcgatatg tgtcagcaac atcaatgttc gacgtgtttc aaccggcggg
      901 actgtctgaa gatggtctcc ttggcggcga tgggaacggg attcctggcc tcattgggcc
      961 aagaaccatc ggccaatgaa ggaccgggct tgacggatta tgtcgatgtg aagacactcc
     1021 ggccaaagcc cgacgtgcgt gtggcggcca ccttcctgga actcccgcgt ccttactggc
     1081 ttggctggcc tgggacaact tatgatctcg acggtcacca aaaggagtac actgcccttc
     1141 tcaacgcat
//
LOCUS       DAOPTR010001204         1148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_97036_length_1148_cov_3.169259, whole genome shotgun sequence.
ACCESSION   DAOPTR010001204 DAOPTR010000000
VERSION     DAOPTR010001204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97036_length_1148_cov_3.169259"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PKI05_16820"
     CDS             complement(<1..488)
                     /locus_tag="PKI05_16820"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOA53919.1"
                     /translation="MTKISEVVHFLGTLCPPILAEDWDNTGLLLGDPELPVEAVMTCL
                     TLTRKTCHEAIDKMANLVVCHHPMPFRPVRCITTVSQSGRILWELANHGIAVYSPHTA
                     FDSCESGINAMLARMLRLHDVRPLRPLPQDPLVGAGRMGCLEQPVSLLEFAHRVKEQL
                     GV"
     gene            758..1118
                     /gene="ssrA"
                     /locus_tag="PKI05_16825"
     tmRNA           758..1118
                     /gene="ssrA"
                     /locus_tag="PKI05_16825"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          275 a          328 c          295 g          250 t
ORIGIN      
        1 ttcaccccca gttgctcttt cacccgatgt gcaaactcga gcaacgaaac cggttgttcc
       61 aaacagccca tccgtcccgc accaacaagt ggatcttgcg gaagcggtcg aagcggtcgc
      121 acgtcatgaa gacggagcat ccgggcaagc atcgcgttaa taccactttc acatgaatcg
      181 aaagctgtat ggggactgta aacggcgatg ccatgattgg ccaattccca cagaatacgc
      241 ccagattgcg acactgttgt tatgcaacgc accggccgaa acggcatcgg atgatgacac
      301 accaccaggt tcgccatttt atcaatagct tcgtggcatg ttttgcgggt caatgtcaaa
      361 catgtcatga cggcctcaac gggaagctcg ggatccccca gcagaagtcc ggtgttgtcc
      421 cagtcttcgg ccaatatcgg ggggcataat gtcccgagga aatggacaac ctcagagatt
      481 tttgtcatga cctgatagcc cttgcgtaaa tcacacctca ggtgtcttaa cctgcgtccc
      541 cgacagacct ttaccatcta ttctcgactt tgagcccgac acatttttgc catctccgcc
      601 gtaataccat tattggaaat gcgccctttt gacgtacggg tcacttcctt ttcctcaacc
      661 cgaagacgac atcacctacc aggcaaaaca tcctcccgaa tgaggcactt gattctcgct
      721 cgatttgtca aataatacaa tagggtggaa cgttatcggg ggcgaactgg gctcgaccgg
      781 acaggttgag gctccggtgg cgcgccgtgg ttgatcggtg gcccacgtaa aaagccgatc
      841 aaacaataag tgccaaccat agcttggctc tggctgccta ataactcggt agccccgact
      901 gcggacccgc gtcggacggg tccaaaagtc ggccgacaaa tccgactcgt tccaggtcac
      961 gccgagcacg cctgggacta aattcctgtt ctcggctggt tgtcggacat cctgtcggtc
     1021 ggaggctccg acaacgagat gaaaaagacc gactacgcgc gtagaagccg gtgctgaaat
     1081 gtcacgggac gcgggttcga ttcccgccgc ctccaatagg gtgtttgcac taggaagcag
     1141 ggttgtga
//
LOCUS       DAOPTR010001205         1146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_97357_length_1146_cov_1.932172, whole genome shotgun sequence.
ACCESSION   DAOPTR010001205 DAOPTR010000000
VERSION     DAOPTR010001205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97357_length_1146_cov_1.932172"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(495..1010)
                     /locus_tag="PKI05_16830"
     CDS             complement(495..1010)
                     /locus_tag="PKI05_16830"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HOA53920.1"
                     /translation="MTLSEELERLHRLHECGALTDEEFATAKARLLKNSADLKPPPLV
                     FPRLRDRLQSGRFWAMLLHLSLLLNFLMPGVALIITFLIWQIGKDRFAVLDEHGKNVI
                     NFVLSYGIYALILFLLVLIPVVGIPVIGAAIVMPMFYLFMAIVALLALFAAWKANQGQ
                     VWRYPLCFRFF"
BASE COUNT          284 a          345 c          293 g          224 t
ORIGIN      
        1 cgggtcgatg ggcaccggcc tctgggcaga ctcgccgcgg gacgctaggc tcgtggcaag
       61 tgagcgctca cacacgcggg cagtcagccg tggaaccctg cggcaaggcc attccagcgt
      121 gatggtcgcc cagtccgcca cccacgattc atccgcccgg ccaaggagat ccgatgcccc
      181 gcacccaggc cccatccacc caatctgttc tttttagatg ctccacgtct ctattattca
      241 ccccgtcgtg gcccgtgtcc aataagagag acaggtctcg cgcccgtttt gcctcgactg
      301 agaacgacag taagagctcg gcaggatagt tgctccaagc gggttgggag agggacggaa
      361 aaccatgaag ttatagagaa tgtaacgcca aacttaccgg ccctcctgat ttcccgatcg
      421 caaccccaaa acttttccca atccgaatgg ttccgttttt ggctaacgtc gccgcctgcc
      481 gtaggggtgt ctcattaaaa gaagcggaaa cacagcggat atcgccagac ctgtccttgg
      541 ttggccttcc atgccgcaaa caaggccagg agggccacaa ttgccatgaa cagatagaac
      601 atgggcataa caatcgccgc accgataacc ggaataccga caaccggaat gagaaccagc
      661 aaaaaaagga tcagcgcata aatgccataa gacagaacaa aattgatcac atttttgcca
      721 tgttcatcca ataccgcgaa gcggtccttg cctatctgcc agatcaagaa tgtgatgatt
      781 agggcgaccc ccggcatcaa aaagttcagt aacagggaca aatgaaggag catggcccaa
      841 aatcggccgc tctggagccg atcgcgcaaa cggggaaaca cgagcggcgg tggtttcaaa
      901 tccgcgctgt tttttagaag ccgcgccttc gcagtcgcga actcttcatc agtcaacgcg
      961 ccacactcgt gaaggcgatg gagccgttcc aattcttccg aaagggtcat gatcatgatc
     1021 ctcccctgct cttgggaatc accacagtcc ttacacgacc cgttgggaga ccactttagg
     1081 ccacaccaag gttgcccaac tcgtggtctc tttactcgat cggctccggt gcctcatcct
     1141 cggggc
//
LOCUS       DAOPTR010001206         1145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_97425_length_1145_cov_3.570642, whole genome shotgun sequence.
ACCESSION   DAOPTR010001206 DAOPTR010000000
VERSION     DAOPTR010001206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97425_length_1145_cov_3.570642"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PKI05_16835"
     CDS             <1..262
                     /locus_tag="PKI05_16835"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HOA53921.1"
                     /translation="LSSVASPKTLLQTLEHFRTVGPTALVLTKLDEAPSFGGLLPVLR
                     SGGLPVSYLTNGQNVPDDIEIAQPSRLARLILGMDNVLRDPS"
     gene            334..>1145
                     /locus_tag="PKI05_16840"
     CDS             334..>1145
                     /locus_tag="PKI05_16840"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase operon protein YhjQ/BcsQ"
                     /protein_id="HOA53922.1"
                     /translation="MDQSGVMYDQAYHLRQLVSRIRPATVDDRQPTLQIRIYGVRQGV
                     GTSTVAEALAHYLHQVGWNVCLFNGVPKNLGKPPAPFRVEHHGQWVETAMSHGDREQQ
                     IPTAFTTRGGREALSGTESRKTLVLRDCGVPNDPKELVSDYPRRCALVTTPAPTDIMK
                     AYALLKRTTGVASPLLPTIVINKSADLSLGQEMAGRLQRACGRFLNRDLTSLFILPIL
                     PRTEGESVNAGEFDEGNWLRRTDGSAQHGSTYCCRREWEETIQLIAKALTEGV"
BASE COUNT          272 a          327 c          314 g          232 t
ORIGIN      
        1 acttagcagc gttgccagcc ctaagacttt gttgcagacg ctggagcact tccgaacggt
       61 cggcccgacg gccctggtcc tcaccaaact ggacgaagcg ccgtcgttcg gcggtttgct
      121 cccagtgttg cggtccggtg ggttgcctgt gagttatctg accaatgggc aaaatgtccc
      181 agacgatatc gagatcgccc agcccagcag actagcccga ctcattttgg gaatggataa
      241 cgttcttcgg gatccctcat aacccagcct taaggagtca gcctcaatca agcaggagcg
      301 tggagtgata cctttggtaa atcatatcgg agcatggatc aatctggggt catgtacgac
      361 caggcctatc acctgaggca gttggtttcg aggattcgcc cggcgacggt tgacgatcgg
      421 caacccacgc ttcagatccg tatttatgga gttcggcaag gcgttggcac ttccacggtt
      481 gccgaggccc tcgcccacta tttgcatcaa gtcggctgga atgtctgcct tttcaacggt
      541 gtcccaaaga atcttggaaa accacccgct cccttccgag ttgagcacca cggtcaatgg
      601 gtggaaaccg cgatgtccca cggtgaccgg gaacagcaaa tacctacggc cttcacaacc
      661 cgcggaggaa gagaagccct atcgggtacc gaaagccgaa aaaccttggt cttgcgagat
      721 tgtggtgttc caaatgatcc caaagaactt gtcagtgatt atccgcggcg atgtgccctc
      781 gtgaccacac ccgcaccgac agacattatg aaggcctacg ccctgctcaa acgtaccacc
      841 ggagtcgcat caccactcct tccgacgatc gtgatcaaca aatcggccga cctctcgctc
      901 gggcaagaaa tggccggccg actccaacgc gcctgtggga ggtttctgaa tcgagacctg
      961 accagtctgt ttattttgcc gatcttaccg agaacggaag gcgaaagcgt gaacgccgga
     1021 gaattcgacg aaggcaactg gctgaggagg accgacggaa gcgcccaaca tgggagcacc
     1081 tactgctgtc gtcgcgagtg ggaagaaacg atccaactga ttgcgaaagc acttacagaa
     1141 ggcgt
//
LOCUS       DAOPTR010001207         1145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_97460_length_1145_cov_2.428440, whole genome shotgun sequence.
ACCESSION   DAOPTR010001207 DAOPTR010000000
VERSION     DAOPTR010001207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97460_length_1145_cov_2.428440"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..>1145
                     /locus_tag="PKI05_16845"
     CDS             78..>1145
                     /locus_tag="PKI05_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOA53923.1"
                     /translation="MNLRPEEAQAFKTALDFAQRQVERLIEKHPDFFPIYTVQGKWYH
                     EGELWTDWTGGFLAGMMWRFYRYTSDEKWLERAIHYSKLLEHRKHDRNVHDLGFIFLN
                     TYLPWYELTKDPALQEVLITAGRTLAMRYQPKGRYLCSFIGPQSLFIDIMMNVPIVFY
                     AAIHTNDDRLMQIALDHCRTTRDRLVREDGSTAHEGLFDVETGEFLGQSTHQGLSAES
                     CWARGLAWSMYGFSKVYGLTQNPEFLEVAEKNAEYWITHLPEDKVPYWDFDADLSQPP
                     PWGRQKESSAAAIAASGLLDLAQQTASPERSVVYRNTALVMLMALSQPEYLAIETPGW
                     EGILKHGVYHTRKNLGVDESVM"
BASE COUNT          270 a          300 c          316 g          259 t
ORIGIN      
        1 cagtggttcc gcggtttcca cgttggcact ataatgatcg gcaatgtttg ttgatttaag
       61 gttaccggag gtgatgaatg aatcttagac cggaagaagc tcaggcattc aagacggccc
      121 tggattttgc ccaacgtcag gtggaacgtc tcatcgaaaa gcaccctgat ttctttccca
      181 tttacacggt ccagggtaag tggtatcacg aaggcgaatt gtggactgac tggaccggcg
      241 gattcctggc cgggatgatg tggcgattct accgatatac ctcggacgag aaatggctag
      301 aacgtgccat ccattactcc aaactcctgg aacatcgaaa gcacgatcgt aacgtccacg
      361 acctggggtt tatctttttg aacacctact tgccgtggta cgaattgaca aaggaccctg
      421 cccttcagga ggtcctcatc acagcaggcc ggaccctcgc catgagatac caaccgaagg
      481 gacggtattt gtgttcgttt atcggacccc agagcctttt catcgacatt atgatgaatg
      541 tccctatcgt cttctatgca gcaatccaca ccaacgatga caggctcatg cagattgcgc
      601 ttgaccactg tcgcacaacg cgcgatcggc tcgtgcgaga agatggctcg accgcccatg
      661 aggggctttt cgacgtggag acaggggagt tcctcggcca aagtacgcat caggggctga
      721 gtgccgagag ttgttgggcc agaggattgg cgtggtccat gtatggcttt agcaaggttt
      781 acggcctcac tcagaaccct gagttcctag aagttgccga gaagaacgcg gaatactgga
      841 tcacacatct ccccgaagac aaggttccct attgggattt tgacgccgat ttgagtcaac
      901 ctccaccttg ggggcggcaa aaggaaagct cagccgccgc tattgccgcc agcggattgc
      961 tcgatctggc tcagcagaca gcatcgccgg agcgaagcgt cgtctatcgc aatacagccc
     1021 tcgtcatgct gatggctctc tcgcagcccg aatatttggc aatcgaaacg ccgggctggg
     1081 agggcatcct caagcatggc gtctatcaca cgcgaaagaa tttgggggtg gacgaatcgg
     1141 tcatg
//
LOCUS       DAOPTR010001208         1144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_97569_length_1144_cov_3.087236, whole genome shotgun sequence.
ACCESSION   DAOPTR010001208 DAOPTR010000000
VERSION     DAOPTR010001208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97569_length_1144_cov_3.087236"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..899
                     /locus_tag="PKI05_16850"
     CDS             267..899
                     /locus_tag="PKI05_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOA53924.1"
                     /translation="MAIRVVIADDHEVIRWGLKEMLRGTEIEVIGEASTGVEAVEKVE
                     NLRPDVVVLDIRFPDLDGLETLAMMREHGLDTRVVLLSSYDYQSYAARAVALGASEYL
                     LKGASRDEILRAIRAAAASQPPAPDSRLGVVAEAMQSKTTVEDDAVQLTQRETQVLRH
                     IALGLSNREIANSLQISVETVKEHVQNILRKLGVSDRTQAAVWAVRKGLV"
BASE COUNT          253 a          268 c          366 g          257 t
ORIGIN      
        1 aataggaaac acgggcggtc tttttgaggc aaaataacca ttttaggggg aaagcttata
       61 accagacttg gtagtggttt cattgccacc gaaaagtagt actattaata aggcggtcca
      121 ccccttgtgg gtataatcga ccatcttgac gcagggacca ggtaagcaca cctgcgcagt
      181 gaggtgatag ccctttggaa gccagtcgat tgcgtcgatt tggggtcatc ggggtgaaca
      241 gttagagaca tgaggaggcc tttaccgtgg caattcgcgt ggttatcgct gacgatcatg
      301 aggtgatccg ttggggattg aaggaaatgt tgcggggaac ggaaatcgag gtcatcgggg
      361 aggcctcgac cggtgttgaa gcggtggaaa aggtcgaaaa tctacgccct gacgtcgtcg
      421 tgctggacat tcgttttcca gatttggacg gtcttgagac gttagccatg atgcgggagc
      481 acggcctcga tacccgggtg gttctcttgt cctcctacga ctatcaaagc tatgccgccc
      541 gtgctgtagc ccttggggcg agtgagtatc tccttaaagg ggcctcgaga gacgagatcc
      601 tgcgggcgat tcgcgctgcg gcggcaagcc agccgccggc tcccgactcg cggctgggtg
      661 tggtggccga ggccatgcag tccaaaacaa ccgtcgagga tgacgccgtt cagttgacgc
      721 agcgagaaac gcaggtcctg cggcacattg ccctagggct ttccaatcgc gaaatcgcca
      781 attcgctcca aatcagtgtg gaaaccgtca aagagcatgt ccagaacatt ctgcgaaaac
      841 taggcgtcag cgatcgcact caggccgcgg tttgggcggt gcgaaagggc ctggtatgag
      901 cttttggcca atgtcttcct ggggtttggt tggggacgca cagtgcgcca aagcttaaga
      961 agaattaaaa ggggaacgcc attgcgaaaa agagcttcct catggttgga tccccggggc
     1021 tttgcggcct gttttgagca ggactaagga tgaggatgtc ggtggcgata atctcgcagg
     1081 ttgttccagc cacacttgtg gaggatttgg cggggcggat ggcgtcgttt tggccggtca
     1141 ttgc
//
LOCUS       DAOPTR010001209         1141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_98005_length_1141_cov_2.159300, whole genome shotgun sequence.
ACCESSION   DAOPTR010001209 DAOPTR010000000
VERSION     DAOPTR010001209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98005_length_1141_cov_2.159300"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..1121
                     /locus_tag="PKI05_16855"
     CDS             177..1121
                     /locus_tag="PKI05_16855"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOA53925.1"
                     /translation="MNRIKGSRGFTLVELLVVIAIIGILIALLLPAVQAAREAARRAE
                     CTNHLKQWGLAFHNYHDVHKTLPFGAQGSYVALPPGANTARRTTWIVPMWPFVEQKAL
                     YDQWTFDQPFHENPNELLLNTGKAAFPLLNCPSDGKPGRMYQGFEDARRNRPRSNYVV
                     NWGNTNFGQTDITSENIIFGGAPFRYNRVVTFSEIRDGLSNTLFMSEVIWSEKVHDQR
                     GDAYNDDRGCAQFMTYDTPNSTAPDFAQCWNPPTPPDPPCVGPAGGNSAHVAARSRHP
                     GGVNALMGDGAVRFFSNTIDLNTWRALGTAQNGESVQF"
BASE COUNT          264 a          278 c          302 g          297 t
ORIGIN      
        1 accgttaatt gtcaaataat actgtgtgat ttgtacatga acgtcgcttc ggctcgttgc
       61 tggcgcttag gattttgtga aagcaaaaag attgcacccg gttgtttggg tcgttataat
      121 cgcctttggt tgtctggggt tactagggtt cgtggtgatg tgtaggaggt tttcttatga
      181 accggattaa gggtagtcgg gggtttaccc tggtcgaatt gctggtggtc atagccatca
      241 tcggcatctt gattgcgttg ctcttgcccg cggtccaagc tgcccgggaa gcggcaaggc
      301 gggcggagtg tacaaatcat ctcaaacaat gggggcttgc tttccacaac taccacgatg
      361 ttcataagac actcccgttt ggagcccaag ggtcgtacgt cgctttgccc ccaggtgcta
      421 acactgctcg cagaactact tggatcgtac caatgtggcc attcgttgaa cagaaagcac
      481 tctatgacca atggacgttc gatcagcctt ttcatgagaa cccaaacgaa ttgctgctga
      541 acaccggaaa agctgccttt cctcttttga attgcccatc ggatggaaag ccaggacgga
      601 tgtatcaagg gtttgaggat gcaaggcgca atcgaccacg ttccaattat gtggtgaact
      661 ggggaaacac caattttgga cagacagata ttacgtccga aaacatcatt ttcggtggag
      721 ccccattcag atataacagg gtagtcacgt tttctgaaat ccgtgacggc ctttcgaata
      781 ccttgttcat gtctgaggtg atttggtccg agaaggttca tgaccaacgt ggcgatgcat
      841 acaatgatga ccgcggttgt gcgcaattta tgacatacga cacacccaac tcgaccgctc
      901 ctgactttgc ccagtgctgg aatcctccca cacccccgga tccgccttgt gtgggcccgg
      961 cgggtggtaa ttcggcacat gtcgctgccc gcagccggca ccccggcggc gtcaacgctc
     1021 ttatgggtga tggtgccgta aggtttttca gtaatacaat cgacctgaac acatggcgtg
     1081 ccctcggtac tgctcaaaac ggtgaaagcg tccagttcta gaaaagcctt agatgatatt
     1141 t
//
LOCUS       DAOPTR010001210         1134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_98899_length_1134_cov_3.199259, whole genome shotgun sequence.
ACCESSION   DAOPTR010001210 DAOPTR010000000
VERSION     DAOPTR010001210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98899_length_1134_cov_3.199259"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1134)
                     /locus_tag="PKI05_16860"
     CDS             complement(<1..>1134)
                     /locus_tag="PKI05_16860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53926.1"
                     /translation="GHGGNDVPEPPDIQKTEEIAKGDLTTPTAVEQQEEGHASWVPSG
                     ADFFIYEDTSADLTGHDRGVVANVFALSEASARADQLLSVLGLKREVVAGRFISADAR
                     FATTVVVFQLKQDQRAEQLNVLGEPWENVGPVQLRQLAVGPERFAFAVVAENCVAIGQ
                     PEILKRLLSGDHDPNIKAILEQKLKSKPRYLMVIRPTLIRHRGLIAWFDAWPEIDQLW
                     QNLWAGANLFIFSIERSEQQVIWTCEWIAKSPDDKDSLEKSVRGLLTRVLGYREVLAS
                     PQAETEVGHGGGDPDKEKESAKKLAELWLSCLDQSQVQVTDTSVLVTLTLPGDLGSEA
                     GLKELGEIVMGEWRHWAEIAMTRRHGHLGKAMSEYESKQQMFPM"
BASE COUNT          249 a          361 c          262 g          262 t
ORIGIN      
        1 catggggaac atctgttgct tactttcgta ctcggacatc gccttgccca ggtggccatg
       61 acgccgagtc atcgcgatct ccgcccagtg ccgccattcc cccattacga tctcgcctag
      121 ctccttcaaa cccgcctcgg aacccaggtc gcccggcagt gtaagagtca ccaataccga
      181 cgtatcagtc acttgaacct gactttggtc aaggcaagag agccaaagct ccgcaagttt
      241 cttcgcgctt tctttttctt tgtcggggtc accgcctccg tgcccaacct ctgtttctgc
      301 ctgcggcgag gccagtacct ctcgatatcc aagcactctc gtcaagagtc ccctcacact
      361 cttttccagg gaatctttgt catcgggact ttttgcaatc cattcgcatg tccaaatgac
      421 ttgttgttcc gaacgttcga tcgaaaagat aaataggttc gcaccggccc aaaggttttg
      481 ccacaattgg tcgatctccg gccaagcgtc aaaccacgct atcaacccac gatggcgaat
      541 gagcgtgggc cgaatcacca tgagataccg gggcttggat ttcagctttt gctccaaaat
      601 ggctttgatg ttggggtcat ggtcgcctga caataatcgc ttcagaatct ccggctggcc
      661 aattgccacg cagttctctg ccacaacagc aaaggcaaaa cgttcaggac caacggccaa
      721 ttgccgcaac tggaccggcc caacgttttc ccacggctcc cccaagacat tgagctgttc
      781 cgcccgctga tcctgtttca actgaaaaac cacgacggta gtcgcaaacc gcgcgtctgc
      841 cgagataaac cgccccgcca ccacttcgcg tttgagccct aaaaccgata aaagctgatc
      901 cgccctcgca gatgcctcag ataacgcaaa gacattagcg acaacgcccc gatcatgccc
      961 ggttaaatca gcgctggtat cttcgtagat aaagaagtcc gcaccactgg ggacccaaga
     1021 ggcgtggccc tcctcttgtt gttccacagc cgtgggcgtg gtcaagtcgc cttttgcgat
     1081 ctcctccgtc ttttggatat ccgggggttc gggaacatca ttgcccccat gccc
//
LOCUS       DAOPTR010001211         1127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_99863_length_1127_cov_3.184701, whole genome shotgun sequence.
ACCESSION   DAOPTR010001211 DAOPTR010000000
VERSION     DAOPTR010001211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99863_length_1127_cov_3.184701"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1127
                     /locus_tag="PKI05_16865"
     CDS             <1..>1127
                     /locus_tag="PKI05_16865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53927.1"
                     /translation="GGELALFDGLEFDVLFHTTGAGRRPVDCNADGKADGGVIEGVNV
                     YGIGVIRFLEALRARLGEHKLILADGQLDARTQRGFGLVNGIESEGFPWLRDWEMKQW
                     STGINRHNFWAQFGRPPVFNYINHKYVRPGTQPGGEATPDVPFSIHRLVFAAAVMTDA
                     AICYSFSPPKDPSSVIGVWDEFWAGQERRLGWLGRPLAPTQTLATQAPDLLAPVLNQL
                     KQKNANFGVLGARTRWEESGFVVEADEEGKDVVFEIPITISAREAYIECVASAQPRRG
                     YPENMARYATCSLVPTETQDTRTLVGSVGEQGTWVGRKPFSAGFYFPELKPGNYHLRL
                     RVEGAEPVRVERLALYPAADARYRVFQHGLVLANPSLHEVS"
BASE COUNT          229 a          299 c          346 g          253 t
ORIGIN      
        1 caggcggcga attagctctg tttgacgggc tcgagttcga tgtgctattt cataccacag
       61 gcgccggacg ccgtccggtc gattgcaatg ccgatggcaa agccgacggc ggcgtgattg
      121 aaggtgtcaa tgtgtacggc atcggagtca ttcgcttcct cgaagctcta cgggcacggc
      181 tcggggaaca taagctcatc ctggccgatg gccaactaga cgcacgcaca cagcgaggat
      241 ttggccttgt caacggcatc gaaagtgaag gttttccgtg gttgcgcgac tgggaaatga
      301 aacagtggtc cacggggatc aatagacaca acttttgggc ccagttcggg cgacctccgg
      361 tgtttaacta tatcaaccat aaatatgtgc gaccgggcac gcagccgggc ggcgaggcca
      421 cgccggacgt gccgttttca atacatcgcc tagtatttgc ggctgccgtc atgacggacg
      481 cggccatctg ttattctttc tctccgccaa aagatccatc ctctgtgatc ggcgtatggg
      541 acgagttttg ggcaggacag gagagacgtt tgggctggct tggtcggccc ctggcaccta
      601 cccagacgct ggcaacccag gcacccgacc ttcttgctcc tgtgctgaat caattgaaac
      661 aaaagaacgc aaactttggc gtattggggg ccagaactcg ttgggaggaa tcagggtttg
      721 tggtcgaggc cgatgaggag ggaaaggatg ttgtgttcga gatacctata acgatttcgg
      781 cccgcgaggc ctacatcgag tgtgttgcca gtgctcaacc ccgccgaggc tatccggaga
      841 acatggcgag gtatgcgacg tgtagccttg tccccacgga aactcaagac acgcgcacgt
      901 tggtcggctc ggtcggcgag caggggacgt gggttggtcg caaaccgttt tctgctggct
      961 tttattttcc cgagctgaag ccggggaatt accatctccg tttgcgtgtc gaaggtgcgg
     1021 agccagttcg tgtggaaagg ctagcgcttt atcccgccgc cgatgctcgc taccgtgtat
     1081 tccagcacgg gctggtgctg gccaatccat ccttgcatga ggtttcc
//
LOCUS       DAOPTR010001212         1126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_99984_length_1126_cov_3.822596, whole genome shotgun sequence.
ACCESSION   DAOPTR010001212 DAOPTR010000000
VERSION     DAOPTR010001212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99984_length_1126_cov_3.822596"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PKI05_16870"
     CDS             complement(<1..330)
                     /locus_tag="PKI05_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOA53928.1"
                     /translation="MSLIRGISDRQSFPVGEMTANAVDRGTATVDLNIVDEIVERCGR
                     KPEAAIPILQAIQAHFRYLPQEALERVCQLTEIRPSQLIGVATFYGQFRFRPVGRHII
                     HVCHGTAC"
     gene            complement(442..>1126)
                     /locus_tag="PKI05_16875"
     CDS             complement(442..>1126)
                     /locus_tag="PKI05_16875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HOA53929.1"
                     /translation="PDDDLSILAALEGASGNVKWEVKRPTGNAWTSPIIVSSAGKDQL
                     ITVADPFVISYDPLTGKEWWRFEGTTGDCGPSPVVHRDLVIAGGEYSYYMFAIRADGT
                     GDVTETHKVWEGEEALPDACSPLAFDHYVLWMSSGAVLACYDIEKGEMLWEHEFPENT
                     FYASPALADQKVYLFSKEGPCFVGVVTDEEFKIEHTNELGEKCVTSPAFQPGRIYVRG
                     EEHLFCLGQ"
BASE COUNT          276 a          340 c          261 g          249 t
ORIGIN      
        1 gcaggccgta ccatgacaaa catggatgat atgacgcccc accggtcgaa aacgaaactg
       61 gccatagaac gtggccaccc cgatcaactg agacggacgt atttccgtca gttggcaaac
      121 tcgttccaag gcctcctgcg gcaaataccg gaaatgggcc tggatcgcct ggagaatcgg
      181 tattgctgcc tccggcttcc gaccacaacg ctcgacaatc tcatcgacaa tgttcaaatc
      241 cacggtggcc gttccccgat caaccgcgtt cgcggtcatt tccccaactg ggaacgattg
      301 gcgatcagaa atcccccgaa tcagtgacat cgatatggct attcctcttc tggtcgttta
      361 aacagctttg cggaaggaca tggcccaact gaaatccgtg cgaccaagcc aggccatttt
      421 tgtcatatgc tattgtctat tttactgccc caggcaaaac aaatgttctt cacctcggac
      481 atagatacgc cccggttgga aggccggact ggtgacacat ttctcaccaa gctcattcgt
      541 gtgctcgatt ttgaactcct catccgtgac cacgcctaca aaacacggtc cctctttgga
      601 aaacagatag accttttggt cggccaaagc gggcgaggca taaaacgtgt tttcaggaaa
      661 ttcatgctcc caaagcatct cgcccttttc gatgtcataa cacgccagga ccgcgcccga
      721 ggacatccac aacacgtaat ggtcaaaggc cagcgggcta caggcatcgg gcagggcctc
      781 ttcgccttcc cacaccttat gcgtctctgt aacatcacct gtgccgtccg cccgaatggc
      841 gaacatgtaa taagaatact caccacctgc gatcacaaga tcacgatgaa ctaccggtga
      901 tgggccacaa tcgccagttg ttccctcgaa tcgccaccac tctttcccgg taagcgggtc
      961 gtaggaaata acaaatggat cggccacggt gatgagctga tccttgcccg cactcgagac
     1021 aatgatcgga cttgtccaag cattcccagt tggtcgtttc acctcccact tcacgttccc
     1081 actcgcgcct tcgagggctg ctaagattga aagatcatcg tcgggg
//
LOCUS       DAOPTR010001213         1123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_100380_length_1123_cov_3.625468, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001213 DAOPTR010000000
VERSION     DAOPTR010001213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100380_length_1123_cov_3.625468"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1123)
                     /locus_tag="PKI05_16880"
     CDS             complement(<1..>1123)
                     /locus_tag="PKI05_16880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53930.1"
                     /translation="SGKIRTIAAAGPGEARGQIPEATPADAVVSWETGFFWKFEEPER
                     LMALQGNVRIVAAAIGRLQCGQLWCWLEPTTDGPLASSQLRPRRIVAQDRVQVESPQI
                     QANTERLESWFVWPASESPNSKADRAAEGFSLGSANNQKATDKDQSPSQLKVTARLLR
                     FEFQITGNRGELSALSADGTVKVQEKPTQPDAQPLEVYGEHLEIIGASTPQTVFALTG
                     NPAFVAGRGLSLSGFQIFLDRGRNLVSTSGPGRVEINQGSQKADLLLPVSTERVEIQW
                     SQGMDFDGQTVTFKGNVQLTSARGRIHAEILEAQISPSIVLSDLKTTQPIQLYAARAV
                     GNVLIENVDVNTQGEVQSRDRLYVSEIAMNWETGAITAPG"
BASE COUNT          234 a          310 c          317 g          262 t
ORIGIN      
        1 ccaggcgccg tgatggcgcc ggtctcccaa ttcatcgcga tttcggagac gtataaacga
       61 tctcgagatt ggacttctcc ctgagtattg acgtccacat tttcgatcag aacatttcca
      121 acagccctcg ctgcgtaaag ttgaatcggt tgcgtggtct ttaagtcgga gaggacaatc
      181 gacggactta tttgagcttc caggatctcc gcgtggatac ggccacgggc gctggtcaac
      241 tggacattac ctttgaaggt gacggtctgg ccgtcgaagt ccattccttg cgaccattga
      301 atttccaccc tctcagtcga aaccggcaga agcagatcgg ccttttggct gccttgattg
      361 atttcgacgc gtccgggacc tgaggtcgag accaagtttc ggccacggtc gagaaagatt
      421 tgaaagccac taagggatag acctcgcccg gccacaaagg cgggattacc cgttagggca
      481 aacaccgttt gaggagtaga ggctccgatg atctcaagat gctcgccgta cacttccagg
      541 ggttgggcgt cgggttgtgt tggtttctct tgaaccttca ctgtcccatc tgcggaaagg
      601 gccgacaatt ctccgcgatt tccggtaatt tggaattcaa accgcaagag tcgtgccgtg
      661 acctttaact gggaaggact ttgatccttg tcggttgcct tctgattgtt cgcgctcccc
      721 aaagagaaac cctctgcggc gcgatcggcc ttgctatttg gggattcgga cgccggccaa
      781 acgaaccaag attccagccg ttcggtgttc gcttgaattt ggggggactc gacctgaacc
      841 cgatcctgag cgacgatgcg ccgcggtcgt agttgggaag aagccaaagg accgtcggtg
      901 gtgggttcta accagcacca caattggcca cattgcagcc ggccgatagc ggcagcgaca
      961 attcgcacgt tgccctgaag ggccatcaag cgctctggtt cttcgaattt ccagaaaaag
     1021 ccggtttccc atgagaccac cgcgtctgca ggcgtcgctt ccgggatttg accacgtgct
     1081 tcgccaggac cggcggccgc aatggtccgg attttgccac tcg
//
LOCUS       DAOPTR010001214         1122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_100510_length_1122_cov_3.560450, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001214 DAOPTR010000000
VERSION     DAOPTR010001214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100510_length_1122_cov_3.560450"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(550..>1122)
                     /locus_tag="PKI05_16885"
     CDS             complement(550..>1122)
                     /locus_tag="PKI05_16885"
                     /inference="COORDINATES: protein motif:HMM:NF027666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4976 domain-containing protein"
                     /protein_id="HOA53931.1"
                     /translation="HSGFWGMGDHTRPLTAFDWTMHIPLIFWCPDHIPSGRQVDLLVS
                     NIDVLPTLVEYLQLTWRKTDARKLPGRSFAALLRGEEIPSWENVVFYEFENVRAIRTD
                     RWKYIERFRQTPNELYDLENDPQELTNLVDDSRCEAVKRELSQRLYQFFDKYADPKWD
                     LWRGGTSKSGLLMHDLFDNPGPAPSPKAKR"
BASE COUNT          259 a          328 c          286 g          249 t
ORIGIN      
        1 ccgtagcccg acgcccgacc ccgccaagaa cgacctcgtg gtgagttaga cggcccccca
       61 agcatgcatg cgtgaaacga tatccctaag cacaaccgtt attgccctgt caaacccgcg
      121 ccggcttgga caccgttttg gcaaagcggg atacgagtat ttcttgtggt gtctggaaac
      181 gtcaagggcc gcatcggcgt ggacaacagt gcgaattgcc ccaaatgagg ccgaccacgg
      241 gatcgtgcca aacggggcaa ccacaggggg ttgcccctac aggtcggtca tcaaaatcgt
      301 aggggcaggc ccctgtgcct gccccagaac ataaacagcg cccggggccc cgtcgaatgc
      361 aaacaacggg tacccgccca agcgcgaacg tgggtggtac gcataaacgg gcaaccacag
      421 ggggttgccc ctacgagccg tttttcgcgc actaccaaac ccatatgaca accgctgtgg
      481 tcgtcgatta ttgtcgcgac aagcgtgagt ggcctgcttt atagcgctgt gttcgggtgc
      541 ttgctttcat caccgttttg cctttggcga cggtgccggg ccgggattat cgaacaaatc
      601 gtgcatcagg agtccagact ttgatgttcc tcctctccaa agatcccatt tgggatccgc
      661 atatttgtca aaaaactgat acaaccgctg cgacaattct cgcttcactg cctcgcatcg
      721 cgaatcgtcc accaagtttg tcaactcttg aggatcattt tccaagtcgt aaagctcgtt
      781 tggcgtctgc cgaaatcgtt cgatgtactt ccaacgatcg gtacggatag ccctcacgtt
      841 ctcaaattca taaaagacca cattttccca ggatgggatc tcctctcccc ggagtagggc
      901 tgcgaagctc ctgcccggga gtttccttgc atcggttttt ctccaggtca attgcaggta
      961 ctcaaccaaa gtgggcagca catcaatgtt acttacaagc aggtccactt gtcttccact
     1021 tggaatatga tccggacacc agaagatgag aggtatatgc attgtccaat cgaacgcagt
     1081 caacggccgg gtgtggtcac ccatgcccca gaatccacta tg
//
LOCUS       DAOPTR010001215         1117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_101201_length_1117_cov_3.198682, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001215 DAOPTR010000000
VERSION     DAOPTR010001215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101201_length_1117_cov_3.198682"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PKI05_16890"
     CDS             complement(<1..312)
                     /locus_tag="PKI05_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA53932.1"
                     /translation="MDRQRHAGIGLSFWPRIFLIIIVSLVAIGQTTIGAELAEQDRHT
                     SLQSGVVNAGEVKPTATRPNILWLTLEDVNCHFGCYGDSYATTPNIDRLAKEGIRYTN
                     AI"
     gene            852..>1117
                     /locus_tag="PKI05_16895"
     CDS             852..>1117
                     /locus_tag="PKI05_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53933.1"
                     /translation="MDFSPRLADIKQRLFPFPLEIWDGHTHLLGLPGKTPEERLERLL
                     ECASRLGIGRVCVFMGTKWQYDPTPDEMQEANNEVRRAIERFPSR"
BASE COUNT          251 a          312 c          283 g          271 t
ORIGIN      
        1 aattgcattt gtgtagcgga ttccctcctt ggccagacga tcgatatttg gtgtggtggc
       61 gtaggaatct ccataacagc cgaaatgaca gttgacgtcc tccagcgtca gccacaggat
      121 attcggccgg gtcgctgtag gtttaacctc ccctgcattg acaacacctg attggagact
      181 tgtgtgtcta tcctgctctg cgagctcggc accaatcgtg gtctggccta tcgcaaccaa
      241 actcacaata ataatcaaga atatccttgg ccaaaatgac agcccaatgc ctgcatgacg
      301 ttgccgatcc atgtggtcgt tccccctccg gcttctccct tttgctcccg taaatcctaa
      361 gtctaaggca agaaacctca gtcaaaggcc ctgacaaacg tttccgctca tctcatccgg
      421 acgtgaccca cctcaagctc ttgtgatcca ccgcacctaa caatacaata ccggcggtcg
      481 tgccgtgagt aaaccgccga gaggacatgg gttccgcttt ctccaaggct atcgcaggat
      541 taccagatca tttttcagtt cggaggaagg cgctgtcatt tccagggcga acagccgtgt
      601 gatttgagta tccttcttgg tagtatgtgc taagagacta tcctatcacg tcccctatcc
      661 cgttcggcga gagaggggcc tgggaactgg cttgaatcgc tcctgttttt ttccatcgag
      721 tccaacgtgg gtttaccctc acctcaaccc tctcctagag ggagagggaa ctttgggttg
      781 ggctttaagc cgcggatgag ttcaagtgag atctgattga cgaagatttc cggtagtaag
      841 gcgctaggac aatggatttc tcaccacggc ttgccgacat caaacagcgg ttgtttccat
      901 tcccgctgga gatttgggac ggacacactc atctccttgg attacccggc aagacgcccg
      961 aagagcggct cgaacgcctc ttggagtgtg catcgcgctt agggattggc cgggtatgcg
     1021 tcttcatggg cacaaagtgg cagtacgatc ccacaccaga cgaaatgcag gaagccaaca
     1081 acgaggtccg ccgggccatt gaacggtttc cctcccg
//
LOCUS       DAOPTR010001216         1117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_101212_length_1117_cov_2.766478, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001216 DAOPTR010000000
VERSION     DAOPTR010001216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101212_length_1117_cov_2.766478"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="PKI05_16900"
     CDS             <1..967
                     /locus_tag="PKI05_16900"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOA53934.1"
                     /translation="FARFGIAEAHQPFTAAQALEKIKAGRKIPVILDTDIGDDIDDTW
                     ALVLLLKSRQLDVRLVVGDNRNALYRGKLIAKLLEVAGRTDIPVGLGVGLEDRPGRQS
                     PWIGDYDLKKYPGKVHEDGVQAIIDTIRSSPEPVTLICIGPVQNIAEVLKRAPDVAQN
                     ARFVGMHGSVYKGYGGSKEISAEYNVKVDPKALQAVFAASWEVTITPLDTCGLVHLTG
                     EKFRKVYESSDKLVRAVMDNYQVWRETQAKKQLPPPERSSTLFDTVAIYLAQSEELTE
                     METLSLTVTDDGFTRIDPNGRPVRAAVRWKDLGAYEDWLVEHLIS"
BASE COUNT          250 a          310 c          321 g          236 t
ORIGIN      
        1 attcgcccgg tttgggattg ccgaagctca tcaaccgttc accgctgccc aggccttgga
       61 aaaaatcaag gccggcagga aaatcccggt gatccttgat accgatatcg gggatgacat
      121 cgacgacacc tgggcgttgg tccttctttt gaaatcccgg caactcgatg tacgacttgt
      181 ggtgggtgac aatcgcaatg ctctctatcg ggggaaactc atagctaagc tgcttgaagt
      241 ggccgggcga acggacatcc ccgtaggctt gggcgttggc ctcgaggatc gacccgggcg
      301 gcaaagcccc tggatcggcg actacgattt aaagaaatat cccggtaagg tccatgaaga
      361 tggcgttcaa gccattatcg acaccatccg tagcagtccg gaacccgtga ccctaatttg
      421 cattggaccg gtgcaaaaca ttgcggaggt cctcaagcga gcgccagatg tcgctcaaaa
      481 tgcccgcttt gttgggatgc atggaagcgt gtacaagggc tacggcggaa gcaaagagat
      541 ctccgctgag tacaacgtga aagttgatcc caaggccctt caagccgtct ttgcggcaag
      601 ctgggaagtc acgatcaccc cgctggatac gtgtgggctg gtccatctga cgggcgaaaa
      661 attccgcaag gtctatgagt cctcggacaa gcttgtccgt gctgttatgg acaattacca
      721 agtctggcgg gaaacgcagg ccaagaagca gcttccacct ccagagcgaa gcagtaccct
      781 ctttgatacg gtggctatct acctcgctca atctgaagaa cttaccgaga tggaaacact
      841 ctcgctgacc gtcaccgacg acggtttcac gcggatcgat cccaacgggc gacccgttcg
      901 ggcggctgtg cggtggaagg atttgggcgc ttacgaagat tggttggtgg aacacctcat
      961 cagttaaacg cccacagtcg ttcccggccg cgacagtgcg tggtccccca gcacttcgtc
     1021 gtgcattggt agacgcaaac ccctggattg ccccgttatt taacgctgtg cgcggggttc
     1081 cctcagttct gaaggggcgc gcgtcgtcgc atccgct
//
LOCUS       DAOPTR010001217         1117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_101223_length_1117_cov_2.506591, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001217 DAOPTR010000000
VERSION     DAOPTR010001217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101223_length_1117_cov_2.506591"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PKI05_16905"
     CDS             complement(<1..181)
                     /locus_tag="PKI05_16905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOA53935.1"
                     /translation="MFQCESRNHSESLATDRRSFLKSAGTLGMGAATVLTGVQVFGEA
                     SPSQQIVLGIVGCGGR"
     gene            complement(264..737)
                     /gene="tadA"
                     /locus_tag="PKI05_16910"
     CDS             complement(264..737)
                     /gene="tadA"
                     /locus_tag="PKI05_16910"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009101290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOA53936.1"
                     /translation="MGDEDIHVHFMRQALREAEQAKHENEVPIGAVIVKNGRIIAAAH
                     NQREQLKDPTAHAEMIAITQAAEAIQSWRLEDCTLYVTLEPCPMCAGAIIMARIPVVV
                     YGADDPKGGAVRSLYRLLEDPRLNHRCVVISGVLAMECGTILTQFFQSQRAMGKK"
     gene            complement(886..>1117)
                     /locus_tag="PKI05_16915"
     CDS             complement(886..>1117)
                     /locus_tag="PKI05_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53937.1"
                     /translation="SRGHSDNGPSGVGRVAKLCHVTTLLGKILCPKTCALEKTRALIC
                     ISPAIYWAHPTDGASWHAYHCPKKRFVLKGFA"
BASE COUNT          267 a          319 c          270 g          261 t
ORIGIN      
        1 ctcgaccacc acacccgaca attcccaaga cgatctgttg gctgggggac gcctcgccaa
       61 agacctggac cccggtcagc accgtggcag cgcccatgcc taaagtaccg gctgacttca
      121 ggaaggaccg gcgatcggta gcgaggctct cactatggtt acgagattcg cattgaaaca
      181 tagctcgacc tcctctggaa aacccattga ttcgtggtct ccccaacgat agggattatc
      241 tcaagaatcg cggccgaggg aaactacttc ttacccatgg cccgctgcga ttggaaaaat
      301 tgcgttaaga tggtcccgca ttccattgcc agcacaccac tgataacaac acaccgatgg
      361 ttcaggcgag ggtcttcgag aagccgatac aaagatcgca cggcaccgcc cttgggatcg
      421 tcggccccat aaacaaccac gggaatccgg gccatgataa ttgcccccgc acacatcggg
      481 caaggttcca atgtgacgta cagtgtacaa tcttccaatc gccaggactg gattgcctcc
      541 gccgcctgcg tgatcgcaat catttcagca tgagccgtcg ggtccttcag ttgttctctt
      601 tggttatggg cggcggcaat aattcggccg tttttaacaa ttaccgcacc gatagggacc
      661 tcattttcat gcttggcttg ttctgcctcc cggagcgcct gccgcatgaa atggacgtgt
      721 atgtcttcat ctcccattga ggcacgctca ttcctttttt ccgaaaatgc aatcatcatt
      781 ttgccaagtc tcgaaggttc tcatggtgtt atgaacaagt ttagtgtgtt caaatgtctc
      841 tagacaggcc atcgatacgc aacgcctgcc gaaatgcagg aaatttcacg caaaaccctt
      901 gaggacaaac ctttttttgg gacaatggta agcatgccaa ctcgcaccgt ccgtgggatg
      961 tgcccagtaa atcgctgggc taatgcatat taaagcccgg gttttttcca aggcacacgt
     1021 tttcggacac agaattttac ccaatagggt tgtcacgtga caaagcttgg ccacccgacc
     1081 gactcccgat gggccattgt cggaatgacc acggctc
//
LOCUS       DAOPTR010001218         1115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_101463_length_1115_cov_3.330189, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001218 DAOPTR010000000
VERSION     DAOPTR010001218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101463_length_1115_cov_3.330189"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="PKI05_16920"
     CDS             complement(<1..660)
                     /locus_tag="PKI05_16920"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOA53938.1"
                     /translation="MMINVFGERDMELATFSTSMRFRYLMRFARPSKLVQVGSTAARC
                     PCRLVTRCVVTMLAAWLVVPIVWCSAQDFEWHTAAPAEVGISREALDELCRALAERRT
                     KAFFVARHDKVVCEWYSPDHGPDRKHGTASLAKAIVGGMALAVALDDGYVTLDEPVSK
                     YIPQWREDPGKAKITIRHLGSHTSGLDDSRPNEEAPWKDAFWRREDPPNNPFTISRDQ
                     AP"
BASE COUNT          262 a          321 c          290 g          242 t
ORIGIN      
        1 cggggcttga tcacgagaaa tcgtgaaagg attatttggg ggatcctctc gtcgccagaa
       61 ggcatctttc cagggggcct cttcgttcgg ccgggagtcg tccaaacccg acgtgtgaga
      121 ccccaaatgc cgaatcgtaa tctttgcctt tccggggtct tcacgccatt gcgggatgta
      181 tttcgagaca ggctcgtcca aggtgacata tccatcatca agtgcgacgg ccaaggccat
      241 acccccaaca atcgcctttg ccagcgaggc cgtaccatgc tttcgatcgg gaccatgatc
      301 tggagaatac cattcgcaaa cgaccttatc gtgacgggcc acaaaaaagg cctttgtcct
      361 ccgctcggcc agggcccggc aaagttcatc gagggcctct cgggagatac ccacctcagc
      421 cggggccgcc gtgtgccact cgaagtcctg ggcagaacac cacacaatgg gcaccaccag
      481 ccacgcggcc agcattgtca ccacgcagcg agtcacaagt cgacacggac atcgagcggc
      541 cgtcgatcca acttggacca atttcgatgg tctggcgaat cgcatcaagt aacgaaaacg
      601 cattgatgta gaaaacgttg ccaattccat gtcgcgctcc ccgaaaacat tgatcatcat
      661 gacacaacgt ctcgcaagtt tgtcagttta cggacaatct tcaacccaac tccccgcaag
      721 gagcaagaac ggcgttctcc acgggcgttt tttctcatgg attgacgttc tcacaccgcc
      781 cgcacacagg atgccgctca cggccggttc cattagaaaa ccggcaacaa cagacgtcaa
      841 gcgtttgcct ttggtcgttc gttcacttcg ttcgagagct tgtgggcgag gtaggcacgc
      901 tattttcgat tggaatcgat tgcgctcttc aacaaggatc aaaaagtgtt ccccttttca
      961 gagggctatg gccgacacaa atctttcctc gtcttgtgaa aggcggcaat tatgctgttg
     1021 gtccgtcaac gcacaattgg tagaattcta gaggtgaaag tgagcaaggc ccttcggggc
     1081 gggcgtgctg ctaattcgcg ctggccaatc gagga
//
LOCUS       DAOPTR010001219         1110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_102149_length_1110_cov_3.823697, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001219 DAOPTR010000000
VERSION     DAOPTR010001219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102149_length_1110_cov_3.823697"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PKI05_16925"
     CDS             <1..199
                     /locus_tag="PKI05_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53939.1"
                     /translation="RNRSDFLTARCIEWGNLRWLPRFYQVGHTPAPAKYGFRLHNSSA
                     SGNKGKKVTLLMRDGISVSRE"
     gene            205..1101
                     /locus_tag="PKI05_16930"
     CDS             205..1101
                     /locus_tag="PKI05_16930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53940.1"
                     /translation="MTSQRVVRACVVVFGLLAFPARVWAEGFPTLVLWSTVKVMAPGS
                     TATGFLVRSPSGEGVILVSADHVFRAMKADECTVLLHLRDESGKLTKNPQKLSVRQGG
                     QPLWVKHPKADVAAISVPFGPEQNLAVLPWEALATAEELLQMELQPGDLVRSVGYPHG
                     NMFKGNDLEFAVVRLGCLATHPFPPSEQASTFLIDSNTFEGDSGGPVYVWKLPGAPGL
                     QREDNGWEKIIGLIVAQHFVDEQYTLIYQSGKFRHRMGLAIVLPSVTIRETVEQAIQD
                     RESKKQPDTPKPQEPVEVTPES"
BASE COUNT          247 a          277 c          329 g          257 t
ORIGIN      
        1 acgaaatcgc agcgatttcc taacggcgcg atgcattgag tggggtaatc tccggtggtt
       61 gccccgcttt tatcaagttg gacacacgcc tgcgccggca aaatacggat ttcgcttgca
      121 caattcgtcg gcgtctggca acaaaggaaa gaaggtcacg cttctgatgc gagatggaat
      181 ttcagtttct agggagtaaa gtacgtgaca agccaacgag tggtgcgagc gtgtgtcgtc
      241 gtttttgggc ttctcgcctt cccggccagg gtttgggccg agggattccc gaccttggtg
      301 ctttggtcca cggtgaaggt tatggcccct ggctctaccg ctacgggatt tctggtgcgt
      361 agtccttccg gggaaggggt gatcctggtt tccgcggatc atgtctttag ggcgatgaag
      421 gccgacgagt gcacggtcct tttgcacctc cgcgatgaat cagggaaatt gaccaaaaat
      481 ccgcagaagt tgagtgtccg gcaaggggga caaccccttt gggtcaaaca ccctaaggcc
      541 gacgtagcag ccataagtgt gccctttggg ccggagcaaa acttggccgt cttgccatgg
      601 gaagcgcttg cgactgccga ggaactgttg caaatggaat tgcagccggg cgacctggtg
      661 cgaagtgtcg gttatcctca tggcaatatg ttcaagggaa acgatctgga gtttgctgtt
      721 gttcgcttgg gatgtctggc cacgcatcct tttccgccct cggaacaagc cagcacattt
      781 ctcatcgact cgaacacatt tgagggcgat agcggtggcc cggtctatgt gtggaaactt
      841 cccggtgccc caggtttgca gcgggaagac aacggttggg agaagattat tggactgatt
      901 gtggcccaac atttcgtgga tgagcaatac accctaatct accaaagcgg taagttccga
      961 catcggatgg gactggccat cgtgttgcct tcggtcacaa ttcgcgagac ggttgaacaa
     1021 gccattcagg accgtgagtc aaagaagcag cctgatactc caaaaccaca agaaccagtc
     1081 gaggttactc cggaatcatg agtcaccggc
//
LOCUS       DAOPTR010001220         1106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_102715_length_1106_cov_3.944814, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001220 DAOPTR010000000
VERSION     DAOPTR010001220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102715_length_1106_cov_3.944814"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1106)
                     /locus_tag="PKI05_16935"
     CDS             complement(<1..>1106)
                     /locus_tag="PKI05_16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53941.1"
                     /translation="PKKGLLDQALVIWPRTNKPLARAMVEAFCRDTGIPGDVPFGELP
                     SHARRLIFHGTGERWFDVYVEDYARIAQGLDGTKSSQQSPEDASSPQLLLRFQYRGLY
                     PCLEAAASSVPSLRTVLSSFITEVECTACAGSRLRDDASAVRFRGRTIDEWCRLPLGR
                     LIAEIRMWQLDERERQIAGEVVREIENRVQFLVDVGLDYLTLARSAPTLSGGESQRIR
                     LAAQIGSGLCGVLYVLDEPTIGLHPRDNRRLLDALRKLRDLGNTLLVVEHDREVINSA
                     DHLLDFGPAAGEDGGQIVAEGHPSTIVHDTRSVTGPYLSGQKAIPVPKNRRMPSWWNE
                     KVAEAAILEDASRQDSPGKKIWRSLTPPGKGWLE"
BASE COUNT          245 a          356 c          287 g          218 t
ORIGIN      
        1 ttccaaccaa cccttgcctg gcggcgtcaa agaccgccaa atctttttcc ccggtgagtc
       61 ctggcgagag gcatcttcaa gaatcgccgc ctccgctacc ttctcgttcc accaggaagg
      121 catccgacgg tttttgggaa cggggatcgc cttttgacca gataggtaag gtcccgtgac
      181 ggatcgagtg tcgtggacaa tggtgctggg gtgcccttcc gccacaattt ggccaccatc
      241 ttctcctgcc gccgggccga agtccaagag atgatctgcc gaattgatga cctcccgatc
      301 gtgctcgacc actaacaatg tgtttcccag gtcccgcaac ttgcgaaggg cgtccagtag
      361 ccgccggtta tcgcggggat gcaggccaat cgtcggctcg tccagcacgt acaggacgcc
      421 acagaggccg ctgccaattt gggcagccaa gcgaattctc tgcgattcac cacccgacag
      481 ggtcggtgcc gagcgcgcca acgtcaaata atccaatcct acgtcaacga gaaactgaac
      541 acggttctca atttcgcgga ccacctcacc cgctatctgc cgttctcgct catccaactg
      601 ccacatgcgg atttccgcga tcaaacgtcc cagtgggagg cgacaccact cgtcaatggt
      661 tcgcccacgg aaacgaaccg ccgaggcatc gtcccgcaaa cggctgccgg cacaggcggt
      721 gcattcgact tccgtaatga aactgctgag caccgtccgc aaactcggca cactactggc
      781 ggcagcctcc aaacaaggat agagaccacg atattgaaac cgtaacaaca actgcggact
      841 actagcgtcc tctggggatt gttgcgagga ttttgtcccg tccaatccct gggcaatccg
      901 tgcgtaatcc tccacgtaaa catcgaacca acgttcccct gtcccgtgga agatgagcct
      961 cctggcatgg ctaggtaact ccccaaacgg cacgtcacca gggatgccgg tgtcgcggca
     1021 aaaggcctcg accatggcac gggccagtgg tttattggtc cgcggccaaa tcaccaaggc
     1081 ctgatccagc agaccctttt tcggat
//
LOCUS       DAOPTR010001221         1106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_102788_length_1106_cov_2.346337, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001221 DAOPTR010000000
VERSION     DAOPTR010001221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102788_length_1106_cov_2.346337"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="PKI05_16940"
     CDS             complement(<1..836)
                     /locus_tag="PKI05_16940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA53942.1"
                     /translation="MMSHHQNWFIVIRTVGRTDLLGRTLHSLVTAGVSRFPVEIVVVE
                     NGGTRRAAELVKSLAEGCRIQYLAIPEVGQAAASNAFLNRNPGTFAWFLDDDVRVTAE
                     AIEAYHLAAQRGQQGREYYGGPLGIDYETPPPEWLLTYLPRSARGWQPNMEDVATTVP
                     MFLGANWAAWADDLLAVGGFENQFGPGGVGAETALQKKLLQRGMRPVYLPQGLVYHWV
                     PRSRCSPQWVLRRAYQIGVAEGLEQPGSEVFLFGYPRWAYRGLMERFSRWCVTRFSRD
                     VHR"
BASE COUNT          259 a          336 c          277 g          234 t
ORIGIN      
        1 cgatggacgt ctcgggaaaa acgggtcacg caccagcggc tgaatcgttc catcaatcct
       61 cgataggccc aacggggata accgaataga aagacttcac taccgggttg ctccaaaccc
      121 tcggcaacgc caatctgata cgcccgacgc aaaacccatt gcggcgaaca ccgtgatcgc
      181 ggcacccagt gataaacaag tccctgagga agataaacgg gtctcatccc tcgttggagg
      241 agctttttct gtagggccgt ttccgctccg acgccgcccg gcccaaattg gttctcaaat
      301 ccgcccacag ccagcaggtc atccgcccag gccgcccagt ttgcaccgag gaacatcgga
      361 accgttgttg ccacatcttc catatttggt tgccaacccc gcgctgaccg cgggagatag
      421 gtaagaagcc attccggggg cggcgtttcg tagtcgatgc caagtggccc accgtagtac
      481 tcgcgccctt gttgccctct ttgggcggct aagtggtagg cctcaatcgc ctccgcagtg
      541 acacgcacat catcgtctaa aaaccaggca aacgtccccg gattgcgatt tagaaacgcg
      601 ttagacgccg ctgcttgacc tacctctggg atggccaggt attgaatccg gcaaccctcg
      661 gccaaagact tcaccagctc ggcagcgcgc cgcgtgcccc cgttttccac gacaacgatt
      721 tccaccggga atcgcgaaac accagcagtc acaagcgagt gtaaggtccg tcccaaaaga
      781 tcggtacgtc ccaccgtgcg aatgacaata aaccagttct ggtggtgtga catcattacg
      841 cccgatttgg aaaatcgtta acccttatag gtgttgtgtc tcgccccaac tccggtaggc
      901 agccgatttt cgatcatcgt ttgattgccc ctacagaaag gcactttgag gcaattcttg
      961 tctgggttta taaccccaaa ggacaaaatg tcgcgaagcg tgccatatcc agagaaactt
     1021 gaggaaagcg cccccgctac tgaccgcaat ctttgatttt ttcccattgt ggttagacgg
     1081 catcgattgt gcaaacgttc gtcgcc
//
LOCUS       DAOPTR010001222         1102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_103319_length_1102_cov_2.844317, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001222 DAOPTR010000000
VERSION     DAOPTR010001222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103319_length_1102_cov_2.844317"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1102
                     /gene="thiC"
                     /locus_tag="PKI05_16945"
     CDS             <1..>1102
                     /gene="thiC"
                     /locus_tag="PKI05_16945"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOA53943.1"
                     /translation="GRMVIPANKIHLKKGLRPIGIGIACRTKINANLGNSALQSDVAC
                     ELEKVFCAISLGADTVMDLSTGPDITAIRQRIIDASPVPVGTVPIYQVAEELGDIAEM
                     TPQHFLDVVEQQATQGVDYMTIHCALLREHLPLVSRRVTGIVSRGGALIARWMTVHQA
                     ENPLYTHFDDLCDIMRQYDVTWSLGDGLRPGCLADASDEAQFAELKVMGELGRRARER
                     GCQVMIEGPGHIPMDQIERNIRLQMEWCDEAPFYVLGPVVTDVAPGYDHISSAIGAAM
                     AAWYGAAMLCYVTPREHLGLPTLEDVRQGVVAYKIAAHAADVARHRPGARDWDDALSR
                     ARYRFDWNEQFRLAIDPERARQMWEETARPGTS"
BASE COUNT          225 a          321 c          346 g          210 t
ORIGIN      
        1 gcgggcgaat ggtgataccg gccaacaaga ttcaccttaa gaagggtctg cgccccatcg
       61 ggatagggat cgcatgccga acgaagatca atgccaattt gggcaactcg gcccttcaaa
      121 gtgatgtggc gtgtgaactg gaaaaggtct tttgtgcgat tagcttgggc gccgataccg
      181 tgatggacct ctccacgggt ccggacatca cggcgattcg gcagcgaatc atcgatgcct
      241 cgcctgtgcc cgtggggaca gtccccattt accaggttgc cgaagaactc ggggacattg
      301 ccgagatgac cccgcagcat ttcttggatg tggtggaaca gcaggcaacc cagggcgtcg
      361 attacatgac gattcactgc gccctactac gggagcatct gcctttggta tcgcgacgag
      421 tgacgggcat tgtcagccgc ggcggggcct tgatcgcccg ctggatgacc gtccaccagg
      481 ccgaaaaccc cctctacacc cactttgatg acctctgcga catcatgcgg caatatgatg
      541 tcacgtggag cctcggagac gggcttcggc cagggtgtct tgctgatgcc agcgatgagg
      601 cccaattcgc ggagttgaaa gtcatgggtg aactcggtcg ccgggcccgt gagaggggct
      661 gccaggtcat gatcgaaggg ccgggacaca tccccatgga ccagattgag cggaacatcc
      721 ggctccaaat ggagtggtgc gacgaggccc cgttttacgt cctgggaccg gtggttaccg
      781 acgtcgcccc cggatacgac cacatctcaa gcgccattgg ggctgccatg gccgcttggt
      841 acggggccgc catgctctgt tatgtcacac ctcgagaaca cctgggcctg cccacactgg
      901 aggatgtccg tcaaggcgtt gtggcgtaca agatagcggc ccacgctgcc gatgtggcac
      961 ggcatcgacc aggtgcccgc gactgggacg acgccttaag ccgcgcccgt taccggtttg
     1021 attggaacga gcaattccga ttggccatcg atccggaaag ggcacggcaa atgtgggaag
     1081 agaccgcccg ccctggtacg tc
//
LOCUS       DAOPTR010001223         1102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_103321_length_1102_cov_2.782235, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001223 DAOPTR010000000
VERSION     DAOPTR010001223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103321_length_1102_cov_2.782235"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="PKI05_16950"
     CDS             complement(<1..1092)
                     /locus_tag="PKI05_16950"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOA53944.1"
                     /translation="MDDDGNPVTLHRLANKPVILEFWSKNHPYSQIVLRMIQAVYEQF
                     KERAAFYAVSIDPPIDTLDSRAVSNQELLNLLQSWSVSIPLLRDPQNDAEKCFDVYIR
                     REAATIPCLVILNAEGVIQAYHLGSAPDLVDRLGATLEKLLSGVNLAEEEKSGFQKMR
                     EELARLVAESEKHGLFTFPVEEITAAGTAQAAEATQPQHLRLTPLFQCTAVKSPGNVM
                     IVETSDQESAIYVVEDGQMATELTWEGQVKVQHPLNPIPPDKINFLRTGVDGQGNRFF
                     VAWSLGSQRVYFFDKNFAAFSRYPADDSPPHDGVADVHLVDLQGDGTIEAVAGFLGLL
                     GVHAFTLDGKRIWSNRTASAAFRVGIMEPN"
BASE COUNT          258 a          309 c          285 g          250 t
ORIGIN      
        1 gttcggctcc atgattccta cacggaacgc cgccgaggct gtcctattgg accaaatcct
       61 ctttccatcg agggtaaagg cgtggacgcc cagcaagccg agaaaaccgg ccaccgcttc
      121 gatcgtcccg tcgccctgaa gatcaaccaa gtgaacatcg gctaccccat cgtgcggggg
      181 actatcatcg gccgggtagc ggctgaaggc agcgaaattc ttgtcgaaga agtagactcg
      241 ttgggatccc agtgaccacg ctacaaaaaa tcgattaccc tggccatcca cgccggtcct
      301 cagaaaattg attttgtcgg gtggaatggg gttcaagggg tgctgcacct tgacctggcc
      361 ttcccaagtc aattctgtgg ccatttggcc gtcttccacg acgtaaatcg cgctctcttg
      421 atcggacgtc tccacaatca tcacgtttcc cggcgatttc accgcagtgc attggaataa
      481 cggtgttaac cgcagatgct gcggttgggt cgcttcggct gcttgggctg ttcccgcggc
      541 agtgatctct tccacgggaa atgtaaacaa tccgtgcttc tcagactcag ccaccagccg
      601 tgcgagctcc tccctcatct tctggaagcc ggatttttct tcttcggcaa ggttgacgcc
      661 gctgaggagc ttctcaagcg tcgctcccaa acgatcgact aaatctgggg ctgaaccgag
      721 atgatatgcc tgaatgacac cctccgcatt aagaatcacc aggcagggga tcgtagcggc
      781 ctcccgccgg atgtacacat cgaaacactt ttccgcgtcg ttttgcggat cgcggagcag
      841 agggatggag acactccatg attggaggag atttaacaac tcctgattgg agaccgcgcg
      901 actgtccaaa gtatcgatcg gagggtcaat actgacagca taaaatgctg cgcgctcttt
      961 gaattgctca taaaccgcct gaatcatcct caagacaatc tgcgagtatg ggtggttctt
     1021 cgaccaaaac tcaagtatca ctggtttgtt ggccaagcga tgcaacgtga cagggttacc
     1081 atcatcgtcc aaaaacagaa aa
//
LOCUS       DAOPTR010001224         1098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_103896_length_1098_cov_2.845638, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001224 DAOPTR010000000
VERSION     DAOPTR010001224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103896_length_1098_cov_2.845638"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            883..>1098
                     /locus_tag="PKI05_16955"
     CDS             883..>1098
                     /locus_tag="PKI05_16955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53945.1"
                     /translation="MRVSDLGLPATAIAPVVTRNNGKTNQGQQISEGTTSQRLQPPEG
                     DQSPKPLHRLAQVRIRQGVSRRTMARRM"
BASE COUNT          248 a          280 c          311 g          259 t
ORIGIN      
        1 ccgacgttcg cttgccctca ccccaaccct ctcccggagg gagaggggga cttttctgga
       61 taggctccct cggataccct cctaagccct gttgcaccca ctgctgaagt aaagtggagt
      121 agtgagggga attgtcaaga aagtggcgaa aacacttttt ccccaactct agtcgcggcc
      181 gtaccttgca aggtaaatca cttcttggca atgacttagg gataaacagc gtttgattcg
      241 aacagagcag aattgtaaac agcctgggtc ctcgctcatt cggcctggtt atttcggggt
      301 gcggccgggc ttgtccacac aatggtcaca attataatgg caaatggaga tagtgtggct
      361 tgctcacagg atggttaggc cgtgttatcc acctgtgatg agggacacgt gtcggccagg
      421 tagcaataat cattatcatc acgaaggcgt cccaccgaga cgaccgattg gtcgatccta
      481 atattcggtg ttgggtgtgt atgacttggg ttgtcagaga gaggagctga aaggacgcta
      541 gaaagaggtc gttgcccgaa gcgtggaaga cagcgtaacc cgttatagtc ctcacaatta
      601 tattttgtgc tcaacggaaa cctcatctcg ggcagacctt ggacaggcgc gcagggttat
      661 gatggatgac cttcctcctg tcagcatagt tgggacgtcc tttctgcgat tgtctctgag
      721 gtggcaagga tcacccaggt gacgtctggc aacccagccg ggatttgagg agtcgctcgt
      781 caagattcat ggcttctcat tggcgctaca tgagcgaccg attcctcttc cggtctttgg
      841 cggtcttttg ccctgtcttt ttggggctaa aggaggaaga ccatgcgcgt ctcggatttg
      901 ggtttgccgg ccactgcgat tgccccagtg gtcacacgta acaacggcaa aacgaaccaa
      961 gggcaacaga tcagcgaggg tacgaccagc caacgtttac aaccccctga aggagaccaa
     1021 tcgccgaagc ctcttcaccg gttggcacaa gtgcggatac gtcagggggt ttctcgtcgc
     1081 accatggccc gtcggatg
//
LOCUS       DAOPTR010001225         1097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_104024_length_1097_cov_3.038388, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001225 DAOPTR010000000
VERSION     DAOPTR010001225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104024_length_1097_cov_3.038388"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            456..914
                     /locus_tag="PKI05_16960"
     CDS             456..914
                     /locus_tag="PKI05_16960"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOA53946.1"
                     /translation="MPVLFYYVHTVQPSEIDPLNHANNVVYVQWLQDAAVAHSAARGW
                     PPERYLALGAGWVVRSHTIEYIHPARLGDEIVVQTWVADFKKVTSRRRYRIYRVNDGC
                     LLAQAETLWAFVTYATGQPTRIHPEVVASFKLPEGDPPPYRPGELSLVKS"
BASE COUNT          220 a          277 c          342 g          258 t
ORIGIN      
        1 atgacttgcg tctggccgaa cagattctca agattttgcc gaagccgcgg gcgtccggtc
       61 cgctccatcc ctttgccgat gacgaccttt ggcgataagg gctcaccgtc gatgaaaaaa
      121 gcacctcaag ctgaagaaaa tgggcaacgg tgtgtgccgc agtgatgagc tggcttggca
      181 tcgcgggtaa actttatcgc gccggtgagc gtagaaaatg atccagtgta gagcctatcc
      241 cataatcttc cctcacgcgt tcggcgggag aggggtcggg ggtgaaagtg gctcgaatcg
      301 ccgccggttt cctcaattga ttccgacgtt ggcttgccct caccccaacc ctctcccaga
      361 gggagagggg gactttttgg gatagacttt taaggaaata ggggttttgt gggtttaagc
      421 gttccgaatg gggtttgccg agaagtgcgt tagctatgcc ggttctgttt tattatgtcc
      481 atacggttca gcccagcgag atcgacccgc tgaaccacgc caataacgtg gtgtatgtgc
      541 agtggttgca agacgccgcg gtggctcatt ctgccgcccg cggttggcca ccggagcgtt
      601 atctggccct tggggccggt tgggtggtta ggtctcacac gatcgagtac attcaccctg
      661 cgaggctcgg tgacgagatt gtcgtgcaaa cgtgggttgc cgatttcaaa aaggtcacct
      721 ctcgccggcg ctatcggatt tatcgggtta acgatgggtg tttgttggcc caggcggaaa
      781 ccctttgggc gtttgtgacg tatgccacgg gtcaaccgac gcggattcat ccggaggtgg
      841 tcgcctcgtt caagttgccg gaaggcgatc cgccacccta tcgaccggga gagctttcgc
      901 tcgttaaatc gtgatggcgg agtcggctga ccgttgtggt gaggatcaag aggtgtgatc
      961 gatcaacaag ttgagtgagg caacgtcctt acggaggaaa gcgatgaagg tcctcgttgt
     1021 cattgggcat ccgcgaccgg gcagtttttg ccatgccatc gccgaaacgg cggtcaagtc
     1081 tctccaaagc aagaacc
//
LOCUS       DAOPTR010001226         1094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_104470_length_1094_cov_3.683349, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001226 DAOPTR010000000
VERSION     DAOPTR010001226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104470_length_1094_cov_3.683349"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..>1094
                     /locus_tag="PKI05_16965"
     CDS             288..>1094
                     /locus_tag="PKI05_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53947.1"
                     /translation="MPRQRSQRLSLVVNPAKAATSFCRGYGWLAWLAGAALVFAFVVT
                     STTLWALEPAPAAVGGSYLLESPMDLSPIGPAGTSTGSQGTISGENTTPPKPPAPSSP
                     GGVTPGTSQPAAVQEVKPEQIYIRDVDGSLKLMLGWTMAQFEELVRLRDGLEQRNQAP
                     PYVIEEIHVQGKCFENYAELSVRVRVRLRGDQPYKIPLRLEQLIPRELPRRVNGQPLL
                     FSYSRQDGGYTLYVQGTADSVEEIIFQGWAPISHVGSEARLVLSVPEAPIS"
BASE COUNT          237 a          284 c          313 g          260 t
ORIGIN      
        1 tatctggaag agtaaaccgt tggcaaggca cgatccatct tctcttgaga tttttgacgt
       61 ctcgaatcat tgttcgatca cgaataggta acggagcacc tattctagag cgattgcgtg
      121 ctgccacaat gacgacacat tccgtcaaaa gacttccttt gcggaaagtc tttggattag
      181 gtaaactaaa tggaaggtcc cgcctcttct gataaggtga aattttgggc ttgtcatcga
      241 cattgtcatt ttagctggga accgaggaag gacttcacga cttggtggtg ccgaggcaac
      301 gcagtcagcg actcagcctg gttgttaacc ctgcaaaggc tgccacgtct ttttgccggg
      361 gctatggatg gcttgcatgg cttgcagggg ccgccctagt ttttgccttt gtggtgacat
      421 ccacgacgct ttgggcgctg gagccggctc cggctgcggt ggggggaagc taccttctag
      481 agtctccgat ggatctttcg cctattggcc cggccgggac atccactggt agccagggaa
      541 caatctcggg agagaatacg acgcccccca agcctccggc accaagttca ccgggaggag
      601 tgacccctgg aacatcccag cccgctgctg ttcaagaggt caagcccgag cagatttata
      661 tccgcgacgt tgacggtagt ctgaaactca tgcttgggtg gaccatggcc caatttgagg
      721 aattggtgcg tttgcgggat ggactagagc agcggaatca ggcgccccct tacgttatcg
      781 aagagattca cgttcaaggg aagtgctttg aaaattatgc cgagttatcg gttcgagtgc
      841 gggttcgcct gcgcggggat cagccgtaca aaatccccct cagactggag caactcatcc
      901 ctcgcgagct tcctcggcgg gtcaatggcc agcccttgtt gttttcctac agccgtcagg
      961 acggaggtta caccttatat gttcaaggga cggcagattc ggtggaagaa atcatattcc
     1021 agggatgggc acccatctct catgtaggga gcgaggcacg actcgtcctc agtgtgcccg
     1081 aggcaccgat ctcc
//
LOCUS       DAOPTR010001227         1087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_105490_length_1087_cov_3.975775, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001227 DAOPTR010000000
VERSION     DAOPTR010001227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105490_length_1087_cov_3.975775"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..>1087
                     /locus_tag="PKI05_16970"
     CDS             23..>1087
                     /locus_tag="PKI05_16970"
                     /inference="COORDINATES: protein motif:HMM:NF016750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53948.1"
                     /translation="MLPKLMPACNTQAKDGTVFVTNSEKVARARAMVEEDLLLRHPID
                     CPICDKAGECRLQDYHFKFGQSERRAEIRPFTSRKRDIGDVFLFVDRCIMCTRCVRFT
                     REITGTSELLVVNRGAHEEIDVLPELPLNNKLSGNVVDLCPVGALADKDFLYKQRVWY
                     LRRHPSVCVLCATGCAIWVEENQDRVYRIKPRENPFVNRWWICNDGRYSYPVIHHPNR
                     LRGAWQRQNGNVSSINWAGAIGQMVDKVQQARQPALCLSPFLTNEEAYLACRWIKQLH
                     PQSTTALGPIPRQGEDERFPGGFVISGEKCPNRLGVEGVAYWFEQPPLRFNDLLARTA
                     EGHFDVIVVLGGYIKPWIDAE"
BASE COUNT          236 a          283 c          313 g          255 t
ORIGIN      
        1 tccaaagacc ggacagattg tcatgctgcc caagctgatg ccggcctgca atacccaggc
       61 gaaggacggg accgtcttcg tcaccaacag tgaaaaagtt gcgcgggccc gcgccatggt
      121 ggaagaagac cttcttcttc ggcatcccat cgattgtcct atttgcgaca aagcgggcga
      181 gtgtcgtctt caggattatc acttcaagtt tggtcagtcg gaacgccgcg ccgagattcg
      241 gccgtttact agtcgcaaac gggatatcgg ggatgtgttc ctttttgtcg atcggtgcat
      301 catgtgtacc cggtgtgtcc gctttacccg agagatcacg ggcacgtcgg agttactagt
      361 agtgaatcgt ggtgcgcatg aagaaatcga cgtcctcccg gagttgccgt tgaataacaa
      421 attatcgggc aatgtcgttg atctttgccc cgtaggggcg ttggccgaca aggactttct
      481 ctataaacaa cgtgtatggt accttcgccg ccacccaagt gtgtgtgtcc tatgtgccac
      541 gggttgtgcc atttgggtgg aggaaaatca ggatcgggtg tatcgcatta agccccggga
      601 aaatcccttc gtgaatcgat ggtggatctg taacgacggc cgatacagtt accctgtgat
      661 ccaccacccg aatcggctgc gcggcgcctg gcagcgacaa aatgggaacg tctcgtcaat
      721 aaactgggcg ggtgccatcg gtcaaatggt ggacaaggtt cagcaagcgc ggcagccagc
      781 cctttgcctt tcacctttct tgacgaatga agaggcctat ttggcctgcc gttggatcaa
      841 gcaactccat ccccagagta ccacagcctt agggccaatt cctcggcagg gagaggacga
      901 acggtttccc gggggctttg tcatttctgg cgaaaaatgt cccaatcgct tgggcgttga
      961 aggcgtggcg tactggttcg agcagccgcc cctgagattt aatgacctcc tcgcaaggac
     1021 agcagagggt catttcgatg tgattgtcgt ccttggcggc tatatcaagc cctggattga
     1081 cgcagaa
//
LOCUS       DAOPTR010001228         1087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_105527_length_1087_cov_2.520349, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001228 DAOPTR010000000
VERSION     DAOPTR010001228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105527_length_1087_cov_2.520349"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..>1087
                     /locus_tag="PKI05_16975"
     CDS             70..>1087
                     /locus_tag="PKI05_16975"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HOA53949.1"
                     /translation="MRQSRKDQRGATISCAYASWKSCFSLIHDKSIVLALLVVFLGVL
                     HCKESPGQNFRRAGQEFAFLRPIELPGGTGGTIGVVEFYHHGQIDPQGRNVIVAAGND
                     LVPVRILQLGPGDFCRLAFQVTSARSPYEILYGGPALPDDRRPTWTSEDGLLLEVREY
                     RDCNLNSFESVKAAFESSRPLGADYVSGVFHAGHPFPGPPGPYMSRYSGMLHIAQTGV
                     YGFYTSSQDCSFLLIDGKVVVSAPGRHPPSYQARPGLRKDIQFTAGAHRFEYYHAATT
                     EVGIAVAAWEVNPKGDQPAPAVIPGEAFRANLIMRGRAGPPMSPAQRFLPDFEFVIVG
                     EVPLP"
BASE COUNT          206 a          289 c          319 g          273 t
ORIGIN      
        1 acctaaatta gtttatggct ctgtacggat caacgccgat cccgtctttg actgggaagg
       61 aaccaattca tgaggcaatc gcgaaaggac caacggggtg caacaatttc gtgtgcctac
      121 gcgtcgtgga agagttgctt ttcgctgatc cacgataagt cgattgtctt ggccttgctc
      181 gttgtttttc tcggcgttct ccattgtaaa gaaagtcctg gccagaattt ccgcagggca
      241 ggccaagagt tcgcgtttct tcgtcccatc gagttacctg ggggaactgg tggcaccatc
      301 ggggtcgtgg agttctacca tcacggccaa atcgatcccc agggtaggaa cgtcatcgtg
      361 gcagcaggta atgatttggt gccggtacgg atccttcagt tgggaccggg ggatttttgc
      421 cgtctcgcat ttcaagtaac gtcggcacga tctccgtatg agattcttta cggcgggccg
      481 gctttgcctg atgaccgtcg gccaacttgg acaagtgagg atggactctt gttggaagtg
      541 cgcgaatacc gggattgtaa cctcaacagc tttgaatccg tgaaggccgc tttcgagtca
      601 tccaggcccc tcggggccga ttatgtttcc ggggtgttcc acgccggtca tccgtttcct
      661 ggtccgcctg ggccttacat gagtcgatat tcgggaatgt tgcacattgc gcaaacggga
      721 gtgtatggct tctacacatc gagccaggac tgtagctttc tccttatcga cggtaaagtt
      781 gtggtatccg cgccggggcg tcatccgccc agctatcagg cgaggcccgg tcttcggaag
      841 gacatccagt tcaccgcggg agcccatcgt tttgagtact atcatgcagc cacgaccgaa
      901 gtgggcattg cggtggcggc ttgggaggtt aaccccaaag gtgatcagcc ggcacccgcg
      961 gtcatccccg gtgaggcctt tcgggccaat ctcattatgc gtggccgggc aggacccccg
     1021 atgagtcccg cgcagcggtt tctcccggat ttcgaatttg tcattgtcgg tgaagttccc
     1081 ttgcctg
//
LOCUS       DAOPTR010001229         1087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_105533_length_1087_cov_2.433140, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001229 DAOPTR010000000
VERSION     DAOPTR010001229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105533_length_1087_cov_2.433140"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="PKI05_16980"
     CDS             complement(<1..859)
                     /locus_tag="PKI05_16980"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOA53950.1"
                     /translation="MTDNQGAWSLGCYGNPDIKTPHIDRLANEGVLFTRCFANNAVCS
                     PTRASFLTGLMPCQHGVHRYLSAGGVQTGPEAYNTLAEFDTLPSLLAQEGYVCGLVGK
                     WHLGNNLSPQEGFSYWVTMPHGHTAGFYNQQIIEGGKIQRYAGYLTELWTQRAIEFIE
                     TQKDRRFFLFLAYNGPYGLGGVLDEPIQNRHRRTYQNAEMKSFPRQPPHPWNHNYANR
                     VGDLSAIRKYAAEISAIDDGVGEVMASLERCGLKHNTLVIFTADQGLAAGHSGFWGMG
                     DHTRPLTAFD"
BASE COUNT          272 a          298 c          287 g          230 t
ORIGIN      
        1 aatcgaacgc agtcaacggc cgggtgtggt cacccatgcc ccagaatcca ctatgccccg
       61 ctgctaaacc ctgatcagcc gtaaaaatga ccagggtgtt atgcttcaat ccgcaccgtt
      121 ccagagatgc cataacctcg cccacgccgt catcaatggc gctgatttca gccgcatatt
      181 tacgtatcgc cgagaggtct cccacgcggt ttgcatagtt gtggttccaa ggatgaggag
      241 gctgcctcgg aaaagacttc atttccgcat tctggtaggt gcgtcggtgt cggttttgga
      301 tgggttcatc tagtaccccg cccaggccat aggggccatt gtaggccagg aagagaaaaa
      361 accgtcgatc tttttgggtt tcgatgaact cgatcgcacg ttgggtccac aattccgtca
      421 aatatccggc gtatcgttga atctttccgc cctcgataat ctgctggtta taaaacccgg
      481 cggtatgacc atggggcata gtcacccagt aagagaagcc ttcttgggga gacagattgt
      541 tgcccaggtg ccattttccg acgagcccac aaacgtatcc ttcttgggcc agcaaggagg
      601 gaagcgtgtc gaactcggca agagtgttgt aagcttctgg gccggtctga actccccctg
      661 cgctgaggta acggtgcacg ccatgctgac aaggcataag ccccgtcaga aagctcgcgc
      721 gtgtcggcga acaaacagcg ttgttagcga agcacctcgt aaacaaaact ccctcattag
      781 ccaagcgatc gatgtgcggc gtttttatgt ctggattacc gtagcaaccc aatgaccagg
      841 ccccctgatt gtccgtcatg atgacgatca cgttaaggga tggcgagtgg gcagaactaa
      901 aggatgaaag cgggaagagt aaaactccca cgagtacaaa aagccacgtt cgcagaagct
      961 cccccgcgca cataacccga cctccaaatc cccccgaaat cggccaacca atccgcaacg
     1021 ccggtgattt tgatgaacgc gggcaagaga ggcaagagag gaggccgaac cagtaatctt
     1081 tatgaat
//
LOCUS       DAOPTR010001230         1084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_105908_length_1084_cov_3.312925, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001230 DAOPTR010000000
VERSION     DAOPTR010001230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105908_length_1084_cov_3.312925"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            518..>1084
                     /locus_tag="PKI05_16985"
     CDS             518..>1084
                     /locus_tag="PKI05_16985"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOA53951.1"
                     /translation="MNGYERVLAMIEGREVDHLPLMPITMMFAADVIGVPYGEYVSDY
                     RVLVEAQLRTAEEYGFDYVSCISDPAREAADCGAVIQYFDDQPPAIVEDQALLADKKK
                     LAQLKVPDPWGGGRMEDRVRAAELFRKKVGGQLFIEGWIEGPCAEAADLRGINTLMLD
                     FYDDPAFVRDLFDLILEMELAFAKAQIEA"
BASE COUNT          222 a          289 c          328 g          245 t
ORIGIN      
        1 ggagggcgat ttgcggctta ttcaggccct tgtcgatgga gaatggaatg aatcggagtt
       61 tctggtagtc cctcccggtg gccgcattgc cccctcgttt gatgacaaca tcatccgccc
      121 ggaaatgctc acggcatgcc ctcgtgaccg gtagggcaac cgttgtggtt accccgtcct
      181 cgtccccaca ccgcgcagca atgaaaggaa cgggcaggca ccaggccctt ccaagtggtt
      241 ggaaggcgtg tagatgatga gcacacgctc tacgttacgg ttggggctgg ggatgtgacc
      301 aaatcgcaag ctagtcgtcg cggtgctatt gattggttag gagctggatc caaagactct
      361 caccggttgc gggaaggccg ttcggctact atgatgaatc cccggcactc cacaatggtc
      421 atcggcggtg tctctccgcg ctgaacacga tcttttgcga cgaccactcg atggaacgcc
      481 caggccagga cattccgacc aaatggaggg gtgattgatg aacgggtacg agcgcgtatt
      541 ggcgatgatt gaaggtcggg aggtggacca tttgccgcta atgcccatta ccatgatgtt
      601 tgccgcggac gtcatcgggg tgccctacgg cgagtatgta agcgattatc gtgtcttggt
      661 agaggcccag ctccgcacag ccgaagaata tgggttcgac tacgtttcct gcatttcaga
      721 ccccgcccgg gaagcggccg attgtggtgc cgtgattcag tacttcgacg atcaaccacc
      781 ggcgatcgtg gaggaccagg ccctgcttgc cgacaaaaag aagctcgctc aacttaaggt
      841 ccctgatcca tggggtggag gccggatgga agaccgcgtt cgggcggcag aattgtttcg
      901 taaaaaggtt ggtggtcaac tcttcatcga gggctggatc gaaggtccct gtgccgaggc
      961 cgccgatctc cgcggtatca atactctgat gcttgacttc tatgacgatc ccgcctttgt
     1021 tcgtgatttg ttcgatctca tcttggagat ggaactggcc ttcgctaagg cccaaatcga
     1081 ggcc
//
LOCUS       DAOPTR010001231         1082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_106210_length_1082_cov_2.966894, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001231 DAOPTR010000000
VERSION     DAOPTR010001231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106210_length_1082_cov_2.966894"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..>1082
                     /locus_tag="PKI05_16990"
     CDS             140..>1082
                     /locus_tag="PKI05_16990"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOA53952.1"
                     /translation="MNSPWRYALCCAGLATIVFFFNLGGPKLWDRDEPRNAGCAREMI
                     ERHDWVTPYFNGELRDHKPVLLYWFMMSAYAVFGVNEFAARFWSAVLGVGTVLLTFDL
                     GRRWFGPRTGFWAGVMLSTCMMFPVAARAATPDSVLIFFSTAALWLYAGPLLESAQSC
                     VSPGNPAAKRSRFWQIVESYALTGLAVLAKGPIGVVLPVGIIFLFHLWRDEPTDAAPR
                     PSTQGVFRKFLSFLHPQRIWAVIRRMNLGLGILVVMLVAAPWYVWVGLRTDGAWLRGF
                     FLTHNVNRFLEPMEGHRGPLFFYPLVFFASFFPWSFLI"
BASE COUNT          173 a          280 c          321 g          308 t
ORIGIN      
        1 aggaaagctc taaccaaaac ttgccttgtc gggggccttt cctagaggta tgatcgggca
       61 acgcctgccc gaatgccaaa gggcgtgaga aagtgggcag ggtgcggggc ctggataact
      121 ttgccacgga gggtgcttga tgaattcccc ttggcgatac gctctttgtt gtgccgggct
      181 ggcgacaatc gtattcttct tcaatctcgg tggtcccaag ttgtgggatc gggacgagcc
      241 gcgaaacgcc ggttgtgccc gggaaatgat tgagcggcac gattgggtca ccccgtattt
      301 caatggtgaa ttgcgggacc acaagcccgt gctgctctat tggttcatga tgtctgcata
      361 tgcggttttt ggagtcaacg agtttgccgc gcgtttttgg tccgccgtcc taggtgttgg
      421 caccgtttta ttgacgttcg atcttggccg tcgctggttc gggcctcgga ccggcttttg
      481 ggccggggtc atgctcagca cctgcatgat gttccccgtt gccgctcggg ctgccactcc
      541 cgattcggta ctcatcttct tttccaccgc agcattgtgg ctttacgcgg gtccactgct
      601 cgaatccgct cagagttgtg tttcccctgg gaatcccgcc gcaaaaagat cgcgattttg
      661 gcagatcgtt gagagctacg cattgacggg cttagcggtc ctggccaagg gccccattgg
      721 ggtggtgttg cctgtgggta ttatctttct ctttcatctc tggcgagatg aacccacgga
      781 tgcggcgccc aggccttcaa ctcaaggtgt tttccggaaa ttccttagtt ttctccatcc
      841 ccagcgtatt tgggctgtga tacgccggat gaacttgggg ctgggaattc tcgttgtaat
      901 gttggtggct gccccatggt atgtttgggt aggattgcgg acggatgggg cctggttgcg
      961 cggctttttc ctgacgcaca atgttaatcg gttcctagaa ccaatggagg gacaccgcgg
     1021 cccgttgttc ttctatccgc ttgtcttctt cgcttccttt ttcccttggt cgtttttgat
     1081 ca
//
LOCUS       DAOPTR010001232         1081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_106327_length_1081_cov_3.938596, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001232 DAOPTR010000000
VERSION     DAOPTR010001232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106327_length_1081_cov_3.938596"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PKI05_16995"
     CDS             <1..187
                     /locus_tag="PKI05_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53953.1"
                     /translation="LFRGAGLLQVYLNDSKGNIVALPINQLGAGEGETWMHKESGTFY
                     FEIVSANCEWEIKVFDK"
     gene            269..550
                     /locus_tag="PKI05_17000"
     CDS             269..550
                     /locus_tag="PKI05_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53954.1"
                     /translation="MADKMSVEEAAKKMGVSPIFLRLGLRHEEFPFGKANVPAKSFPM
                     NLLCFTPLTTECSKHHLLALFSSCKTPDVNTKFLIVEKGVQMVSGAPGS"
     gene            800..991
                     /locus_tag="PKI05_17005"
     CDS             800..991
                     /locus_tag="PKI05_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53955.1"
                     /translation="MSEKTKRELWAERIEAFLASGLSQRAWCQEHGLRQNQLGYWLRK
                     LKPELAMPKKSRWIRDEVQ"
BASE COUNT          278 a          238 c          309 g          256 t
ORIGIN      
        1 gctttttcgg ggggcaggac tgctgcaagt ctatttgaat gactccaaag gaaatattgt
       61 tgcattgccg attaaccagt tgggggctgg tgaaggagag acctggatgc acaaagagtc
      121 agggactttc tacttcgaaa tcgtctcggc caactgtgaa tgggaaatca aggtttttga
      181 caagtaaggc accctcgggg gcttttccgc ctatcagcgg tgagatgcca tttatgctaa
      241 aatacggtgc tgattggagg tggagccagt ggccgacaag atgagcgtcg aagaggctgc
      301 gaagaagatg ggggtcagcc cgatattcct gcggctcggg ctgcgccatg aggaatttcc
      361 gttcggcaag gccaatgtac cagcaaagag ctttcctatg aaccttctct gcttcactcc
      421 cctcacgacg gaatgttcga aacaccacct tttggcttta ttttcctcct gcaaaacacc
      481 tgacgtcaac actaaattcc taattgttga aaaaggagtc caaatggtat cgggggcgcc
      541 tggttcctga actggagcgt ttgttcgacg aacatgattc cgtcatcgaa caccgcccac
      601 aagtcggtgg gtgttttttt gttgcaggaa gttaacaacc ggcgtgaaaa cggttgatca
      661 taatagccgg gcgatcatat gaggaagctg atttgttgtt gctcgtggtt attcttcgta
      721 agcgtcaaag ccggcacacc tccaaactcc tctgctgtga tgggataatg gatccatcaa
      781 gttgaggagt tgagctttta tgtcggaaaa aacgaaacga gaactatggg ctgaacgcat
      841 cgaggcattc ctggccagcg gcctgagtca aagggcttgg tgtcaggagc acggtctaag
      901 gcagaaccaa ctcggttact ggctgcggaa actcaagccc gaattagcta tgccaaagaa
      961 gagccgttgg atcagggatg aagtccaatg aactgagcgg acacgaggat gcaaatgttg
     1021 cggggcatac gggatgaaag ctatgcgtct tgcagtggga cgtcatatgg gcggacagaa
     1081 a
//
LOCUS       DAOPTR010001233         1081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_106384_length_1081_cov_2.410331, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001233 DAOPTR010000000
VERSION     DAOPTR010001233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106384_length_1081_cov_2.410331"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..>1081
                     /locus_tag="PKI05_17010"
     CDS             39..>1081
                     /locus_tag="PKI05_17010"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOA53956.1"
                     /translation="MLSLGYCSEEQIAECLAEEYGLPFTRLEMRLFDQKIVDLLPREY
                     IENNSVLPLFRVRDVLTVAVAEPSNLFLFQEIEHLTGLRVRPIVAPYRDIRRMIASLP
                     NSSVFVIDDILDGSDVAEVTLVEETIEDIGNLAEIAGQSPVIRLVNYILFNAVKEGAS
                     DIHIEPSERCVRVRYRVDGRLQKAIELPAHLGAPITSRIKIMAGMDISERRLPQDGRV
                     NVLLENRKIDLRVSTFPGQHGEKTVIRILDTRHVSLNLDDLGFSEDVLGKLKAAIRAP
                     NGIILVTGPTGSGKSTTLYACLNAISSIEINICTVEDPIEYHLPLVNQFQVQDKIGLT
                     FSKALRTLLRQDP"
BASE COUNT          251 a          281 c          286 g          263 t
ORIGIN      
        1 aacgttcaag tggccgcacc aggctacttg gtgaaatttt gctttccctg ggatactgct
       61 ccgaagaaca gattgcagaa tgccttgcgg aagagtacgg attgccgttc actcggcttg
      121 agatgcgtct ctttgatcaa aagattgtcg atcttttacc ccgggaatat attgaaaaca
      181 actccgtact accgctcttt cgggttcgcg atgtgttgac agtcgccgtg gcagaaccgt
      241 ccaacctgtt tctctttcag gagattgaac acctcacggg gctgcgcgtt agaccgattg
      301 tggctcctta ccgagatatt cgacggatga ttgcgtcgct acccaactcg agtgtctttg
      361 tgatcgacga tatcctcgat ggaagcgacg tcgcagaggt cacattggtg gaggagacga
      421 tcgaggatat cggaaacctc gccgaaatcg ccggccagtc accggtcatt cggctcgtga
      481 actatatttt gttcaacgcg gttaaggagg gggccagcga tattcacatc gaaccgagcg
      541 agcgttgtgt tcgtgtgagg tatcgcgtgg atggtcgttt gcaaaaggcc atcgagttgc
      601 ctgcccatct cggtgcgccg atcaccagcc gaatcaaaat catggctggc atggacatta
      661 gcgaacggcg acttccccaa gatggccgcg tgaatgtcct tttggaaaat cgcaagatcg
      721 acctacgtgt gagcacattt cctgggcagc acggcgaaaa gacggtcatt cggatcttag
      781 acacgcgcca tgtttcgcta aatcttgacg atctggggtt ttcggaagat gttctcggca
      841 agctgaaggc agccatccgg gctcccaacg ggatcatctt agtcacgggc cccaccggta
      901 gcggaaaatc tacgaccctt tatgcctgtc ttaatgccat ttcatccata gaaatcaata
      961 tctgtacggt cgaggacccg attgaatacc atctgccact ggtcaatcaa ttccaggtcc
     1021 aagacaaaat tggcctcacg ttctccaagg cgcttcgcac gctgttgcgg caagatccgg
     1081 a
//
LOCUS       DAOPTR010001234         1079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_106682_length_1079_cov_2.417969, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001234 DAOPTR010000000
VERSION     DAOPTR010001234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106682_length_1079_cov_2.417969"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..>1079)
                     /locus_tag="PKI05_17015"
     CDS             complement(5..>1079)
                     /locus_tag="PKI05_17015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA53957.1"
                     /translation="IHDVEDPRRWTGQPGLTFHDRFYAAFGRDWPLPFGRYRASQWIT
                     RCRTQFANHAWRLARLIDRLRPDIVHSLGLHLGGYLTLAAREECRKPFPTWVVTNWGS
                     DLFLFGRLTEHKERLAKVLNTADYYWCECQRDVELAKSWGVPSERILPTVPAPGGFDI
                     EQAEKLRTPGPTSSRRVITVKGYQDWAGRALVAFQALRGCSDLLAGYRITVYTGGRDP
                     EPVRIAGELLSQDLDIPVTFVPPRTPHEAMLRLHGQSRISIGLSISDSIAISLLEAML
                     MGSFPIQSNTGAAYEWIEDGKTGLIVPPEDPHAVTEAIRRALWEDHLVDSASAINQQV
                     IAERCERRKIAQCVVSTYERICQ"
BASE COUNT          237 a          336 c          289 g          217 t
ORIGIN      
        1 gcaactactg acagattctt tcgtacgttg acaccacaca ctgggcaatc tttcgccgct
       61 cgcatcgttc ggcaatgacc tgctgattga ttgcgctcgc ggaatcgaca aggtggtcct
      121 cccacaaggc gcggcggatt gcctctgtca cggcgtgtgg gtcttccggt ggtacgatca
      181 gcccggtctt gccatcttcg atccactcat aggccgcacc ggtgttcgac tggatgggaa
      241 acgaccccat gagcatggcc tccagaagtg aaatggcaat cgaatcgcta atgctcagcc
      301 caatggatat ccgtgattgg ccgtgcaatc gcaacatggc ctcatggggc gtccgcggcg
      361 gaacaaacgt gactgggata tccaggtcct gagaaagcaa ttcgccagca attcgcaccg
      421 gctccggatc acgaccgccc gtataaacgg taatgcgata tccggctaac aggtcgctac
      481 atccccgcag ggcctgaaag gcaacaaggg cccggccagc ccaatcctgg taacccttga
      541 ccgtgatcac tcgccggctc gacgtcggac ccggcgtccg tagtttttcg gcctgctcaa
      601 tatcaaaacc tcccggcgca ggcaccgttg gcaagatgcg ctcgctgggt accccccaac
      661 tcttcgccaa ctcgacatcg cgttgacatt cacaccaata gtaatcggcc gtattgagga
      721 ccttcgccaa ccgctccttg tgttctgtca gtcggccaaa aagaaacaag tcgcttcccc
      781 agttggtcac cacccacgtg ggaaagggtt tgcggcactc ctctcgcgcc gccaaagtga
      841 gatagcctcc caggtgcaat cctagggaat ggacaatatc aggccgaagc cggtcgatca
      901 gtcgtgccag gcgccaggcg tgattagcga attgcgttcg acatcgcgtg atccattggc
      961 ttgcccgata gcggccaaac ggcaagggcc aatctcggcc aaacgccgcg tagaaccgat
     1021 catggaacgt cagcccaggt tgacctgtcc atcgtcgtgg gtcttccaca tcatgaatg
//
LOCUS       DAOPTR010001235         1078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_106807_length_1078_cov_2.415445, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001235 DAOPTR010000000
VERSION     DAOPTR010001235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106807_length_1078_cov_2.415445"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..>1078
                     /locus_tag="PKI05_17020"
     CDS             155..>1078
                     /locus_tag="PKI05_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53958.1"
                     /translation="MWCSRANKRVQSVIGQGVLVSFLVTVTVVVSATRAFAQGKADNL
                     WQLCRDKAAIHRFSTFFTAQNVRDYLATDEGRQSAIAWCRQTGVTRVFIESFRDGYLV
                     PKDLLAQASHVFRAAGFDVSGCVTTTNVGKPSTGWQIISCYTDRATQDKLAEIFKYTA
                     SLFDEIMIDDFLFCDCQCAECDAARKSRTVNIGERSFPVDSDTWSAYRCELMVQVSRQ
                     LILEASRSVNPNVRIIIKYPQWYDQFHERGYDVVRQTADFDLIWVGTETRDYDNPRWG
                     RKVQYEAYFIMRWLGGIGGEKCGGGWFDPFGT"
BASE COUNT          232 a          254 c          314 g          278 t
ORIGIN      
        1 gagaataggc agatggtgcg ggtctcgggc gatgagccat ccatttcttt cgatgcgaag
       61 cagttaggat ggctcacgca gtccgcggtt tggtgactaa cctcagggag gaatatcccc
      121 tgcttttaac ctacgacagg ccgggaggtg tttcatgtgg tgttccaggg caaacaagcg
      181 cgtccagtcg gtaattggtc aaggagtgtt ggtatcgttt ctcgttacgg ttaccgtggt
      241 cgtctccgcc acaagggcct ttgcccaagg caaagctgac aatctttggc aattatgtcg
      301 ggataaagcc gcgattcatc ggttctccac gttcttcacg gcacaaaatg tccgcgatta
      361 tttggcaact gatgaggggc gtcagtcggc catcgcatgg tgccgccaga ccggcgttac
      421 gcgggtcttc attgaatcat tccgtgatgg ttacttggta cccaaagacc tgttggctca
      481 agcgagtcat gtcttccgtg ctgccggatt tgatgtctcc gggtgcgtga cgacaacaaa
      541 cgtgggaaag ccttccacgg gttggcagat catttcctgt tacactgacc gagccaccca
      601 ggataaactg gccgaaatct tcaaatacac ggcgtcgctc ttcgatgaga ttatgattga
      661 tgattttctc ttttgcgatt gtcagtgtgc agaatgcgac gctgctcgaa agagtcggac
      721 ggttaacata ggcgagcggt catttcccgt ggattctgac acatggtcgg cttaccgctg
      781 cgaattgatg gtccaggtat cgcgccagct tattttagaa gcgtcccgaa gcgtcaatcc
      841 caacgttcgg ataattatca agtatcccca gtggtacgat caatttcatg agcgcggtta
      901 cgacgtggta aggcaaacgg ccgattttga cctcatttgg gttggtacag aaactcggga
      961 ctacgacaat ccacggtggg gacgcaaggt ccagtatgag gcctatttta tcatgcggtg
     1021 gttgggcggt atcggtggcg agaagtgtgg tggcggatgg tttgatcctt tcgggacg
//
LOCUS       DAOPTR010001236         1071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_107838_length_1071_cov_2.955709, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001236 DAOPTR010000000
VERSION     DAOPTR010001236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107838_length_1071_cov_2.955709"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(749..>1071)
                     /locus_tag="PKI05_17025"
     CDS             complement(749..>1071)
                     /locus_tag="PKI05_17025"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOA53959.1"
                     /translation="PNAQPARTAPLGILVCGDLSLELSKGYWVIDCAAATQNILLAAH
                     GLGLGAVWTGVYPRENRVEGLREMLGIPEHVVPHSLVLIGYPAESPAQVDRFDAKRIH
                     YNRW"
BASE COUNT          227 a          306 c          289 g          249 t
ORIGIN      
        1 tcccgtcaga accgtgacaa tcaacgccag ggtcaggaag taatgacgtc gcacgctatt
       61 cacaaaggct gacatggtta ccctcctatc gcttaagtga ttttatcagt taaggtccaa
      121 caacggccgc cagattttga agcaactccc acctcttttc cacgatacac ctacttatcg
      181 cggaatcagg ccccctagac gtggcacgac agcgttctcg ttagcaggct tttagaagat
      241 gttgccagtc cccgatgcct tgtagcaagg aaaccttaga gccgattcca caacctcccc
      301 tcgcccggtt ggcgggagag ggggcggggg tgagggtggc tcgaatcgct gccggttttc
      361 tcaatcgatt ccgaggttcg cttgcgctca ccccaaccct ctcccagagg gagagggaat
      421 tttgggataa tctcttacta actttggaac cgtttcttgc cggaatggga ggccacctcc
      481 tggttatcct tttcctattt tgctcaattg cctacattgc caagggtaaa ccgtccaagg
      541 gttttcgcac agctcacgca tcgtgaacct catctgccaa tccggcgccg gaacctgcca
      601 gaatgggttt tattttgtgc gagattcctg ggacgattta cctgcggtgg ggttactcag
      661 caattgctat gccgaatttg cgagcccaaa cggttacgtt ggcccgccac tcggcccgcc
      721 gcactagacc tgccagcgct tggtgcggtt accaccggtt gtagtgaatc cgtttggcat
      781 cgaaacggtc aacctgggcc ggagattcgg ctgggtaacc aatcagcacg aggctatggg
      841 gaacaacgtg ttccgggatg ccgagcatct ctcgtaatcc ctcgactcgg ttttcccggg
      901 gatagacacc ggtccatacg gcccccagtc ccaaaccgtg cgctgcaaga agaatgtttt
      961 gggtggcagc ggcacagtcg atgacccagt atcccttaga gagttctagg ctcaggtcgc
     1021 cacaaacgag aatccccagg ggtgccgtgc gggccggttg tgcgttagga a
//
LOCUS       DAOPTR010001237         1070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_107963_length_1070_cov_3.994089, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001237 DAOPTR010000000
VERSION     DAOPTR010001237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107963_length_1070_cov_3.994089"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..>1070
                     /locus_tag="PKI05_17030"
     CDS             40..>1070
                     /locus_tag="PKI05_17030"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOA53960.1"
                     /translation="MGLTAEEASKAAPSTALPADEYALPNIPRPIADALQNRRYDDAV
                     KLIDEQLQKPSSVADYLTYLKGRALYLAQKYDEAIAVFLEGEKQFTESPWRHWLQFGR
                     ALALARKGDFASAEAIYRDQAKRLLSLSRKDELAGIYLEFANRLFDPPNKLEEKPDYG
                     KARQFYEKARDLGPGESVLVGILFQIARCHQLSADWNAAIAAYRDFIQRFPQDSREIA
                     ARFHLGECLLNQGNLAEARRVWEDLLAKHQDHRSAETATAAFRLAETRGMPNPPSDEE
                     LGLGVAALRQFLERFPQDERVGQAYLIMAQGFVSREHWDDARRVLEEFLADARSEKAK
                     ERADAMFLLA"
BASE COUNT          228 a          285 c          327 g          230 t
ORIGIN      
        1 gtcggagtgg ttttggcagg cgtcacatgg ggggtttgga tgggcctcac agccgaggag
       61 gcatccaagg cggctccctc cacggccctt cccgccgacg agtatgctct gcccaacatt
      121 ccgcggccca ttgccgacgc ccttcagaat cgccggtatg acgacgccgt caagttgatt
      181 gacgagcagt tgcagaagcc gtcatcagtg gcggattatt tgacctatct taaaggtcgg
      241 gcattgtatc ttgcccaaaa gtacgacgaa gcgatcgcgg tctttctcga aggtgaaaag
      301 caatttaccg aaagtccttg gcggcactgg ctgcaattcg gtcgcgcact ggcgctggca
      361 cgcaaagggg acttcgcgtc cgcggaggcc atctatcggg accaggctaa gcgactgctc
      421 tcgctcagtc gaaaggatga gctggcaggc atctatttgg agtttgccaa tcgtttattc
      481 gatcccccaa acaagttgga agaaaagccc gattacggca aggctcggca attctacgaa
      541 aaggcgcgcg atcttggtcc cggtgaaagc gtgctggtag gcattttgtt tcagatcgcg
      601 agatgtcacc agttatcggc ggactggaac gctgccatag cggcttatcg tgattttatc
      661 cagcgatttc cccaggacag ccgcgagatt gcggcccggt tccatcttgg ggagtgcttg
      721 ttaaaccagg gtaacctggc cgaggcccga cgcgtttggg aggatctgct cgctaagcat
      781 caagaccacc gcagtgccga aacggctacg gcggcatttc gactggcgga aacccgcggg
      841 atgcccaatc cacccagcga tgaggaattg ggcctgggcg ttgccgcctt gcggcaattc
      901 ttggagaggt tccctcaaga cgagcgggtc ggtcaagcat atctcattat ggcgcaagga
      961 ttcgtatcgc gtgaacattg ggatgacgca cgtcgggtgc ttgaagagtt tttggcagac
     1021 gcacgctcag agaaggccaa agaacgggcg gacgccatgt ttcttttggc
//
LOCUS       DAOPTR010001238         1070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_107985_length_1070_cov_3.174384, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001238 DAOPTR010000000
VERSION     DAOPTR010001238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107985_length_1070_cov_3.174384"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PKI05_17035"
     CDS             complement(<1..667)
                     /locus_tag="PKI05_17035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53961.1"
                     /translation="MDSSHVDIQVIERIVREVLADLGRVSPIASEAPGQQSASRTHAG
                     EGKQSTDDVTSANGGTTLPRLARIHSPRDGELTLPNKVITLATLENRLTGVRQIVVPP
                     GAIITPAVRDLLAEKAIKVFFGIGPKSTEVPAKANNETGTPEGASLVILLVTRRVSTE
                     QLENLLRPEGITAAYESLDCLVRASDRVAEVCRSGNLAVVLTRHVSMEVCLANRHKGV
                     RAVA"
     gene            complement(667..924)
                     /locus_tag="PKI05_17040"
     CDS             complement(667..924)
                     /locus_tag="PKI05_17040"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOA53962.1"
                     /translation="MLAGRVVGHATSTVKHPSMQGWRLLLVQPVAADGRSPDGDPILV
                     VDHLGAGRGDLVIVTSDGKHTQTVLKDAHTPVRWSVIGIVD"
BASE COUNT          212 a          336 c          286 g          236 t
ORIGIN      
        1 ctgcaacggc gcgaactccc ttgtgcctgt tagccaagca aacttccatg gagacatgcc
       61 gcgttaatac cacggccaag tttcctgacc gacacacctc ggccacacgg tcgcttgccc
      121 gcacgaggca atccaacgac tcatacgcgg cagtgatccc ttcaggtcga agtaggtttt
      181 ccagttgctc ggtggagact cgccgtgtga ccaacaaaat cacaagggac gcaccctccg
      241 gcgtgcccgt ttcattattg gccttggctg gcacctctgt gcttttcggt ccgatgccaa
      301 aaaacacttt gattgccttc tctgccaaaa gatcccggac cgccggggtg ataatagccc
      361 caggtggaac gacaatttgc cggacgcccg tcaagcgatt ttccagtgtc gccagggtga
      421 tcaccttgtt tggcagtgtg agttcgccgt ctcgtggcga gtgaatccgg gcaagacgcg
      481 gcagagtcgt cccgccattg gcgctcgtta cgtcgtcagt gctctgcttt ccctcgccgg
      541 cgtgagtcct cgaagcgctt tgctgaccgg gagcctctga cgcaatgggt gagaccctcc
      601 cgagatccgc caggacctcc cggacaatgc gctcgattac ttggatgtca acgtgggaag
      661 aatccatcag tcaacgatcc caatcaccga ccaccgcact ggtgtgtggg cgtctttcaa
      721 caccgtctgc gtatgttttc catcgcttgt tacaatgacg aggtcccctc gtcctgcccc
      781 caaatggtcc accaccaaaa tgggatcgcc atctggcgac cgaccgtcgg cggccacggg
      841 ctgtaccaac aggagcctcc agccctgcat ggatggatgc ttcaccgttg acgttgcatg
      901 cccgacgacc cgtcctgcaa gcatgttcga ttgccttcgt cacccgttgt gtgccatcgt
      961 cgatctcgga tcggtctctc gtttaagaat gaaacaacat cacttgccga ggatgcggag
     1021 actgtcgatc atggcacagc gacgttcccg ggtgaatgtc aacggcgtcg
//
LOCUS       DAOPTR010001239         1070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_108083_length_1070_cov_1.852217, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001239 DAOPTR010000000
VERSION     DAOPTR010001239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108083_length_1070_cov_1.852217"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PKI05_17045"
     CDS             complement(<1..190)
                     /locus_tag="PKI05_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA53963.1"
                     /translation="MACADVHRLNAENFAKPFEGRCQVYTDYRRVLDRKDVDAVTIGT
                     PDHWHTKIAIDAMKAGKDV"
     gene            complement(212..388)
                     /locus_tag="PKI05_17050"
     CDS             complement(212..388)
                     /locus_tag="PKI05_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOA53964.1"
                     /translation="MGRFKATRRDLLKGSIGAAAAVFVPHVWVTPFSWAESPNERLNI
                     AAIGVGGRGSDIGR"
     gene            714..>1070
                     /locus_tag="PKI05_17055"
     CDS             714..>1070
                     /locus_tag="PKI05_17055"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOA53965.1"
                     /translation="MEAVTMSLRPSQTKIVATLGPASDSYETICSLIRSGVDVFRLNA
                     AHGSLDEHGERLRRIRQAEREIGWPVGVLVDLGGPKIRLGELVGGSLECTPGMKVRFV
                     KNDKAERLSDLTSTYPS"
BASE COUNT          258 a          308 c          284 g          220 t
ORIGIN      
        1 aaacgtcctt gcccgccttc atggcgtcga tggcaatctt cgtgtgccag tggtcgggcg
       61 tgccaatggt tacggcatcg acatccttgc gatccaacac ccggcgatag tccgtataaa
      121 cctgacatcg cccttcaaat ggcttggcaa aattctcagc gttcagacga tggacatcgg
      181 cgcatgccac caagttcccc aactgtgcgg cctagcgccc gatgtcgctt ccgcgaccac
      241 caacaccaat ggcggcgatg ttaagtcgct cgttcggcga ctcggcccaa gaaaacggtg
      301 tcacccaaac gtgcgggaca aaaacggcag ccgccgcacc gatactccct ttcaaaagat
      361 cacgccgtgt ggccttgaaa cgacccatcg aaacctcctc actattggga cttggaaagc
      421 cgttgcggaa agccaatcgc aagaccaaat tataacaata atcccatccg ttgcgattct
      481 caacttgccc ggcaaacagt ttgtatcata aggttgtgct cggttacccc gaggaactgg
      541 tttctccaag gtgcgatgaa cgcgggccac cgatcgagaa cataaaaaag aacaatactg
      601 ttcggcgccg tcctcgcaat actacggcga actctgggtg ccggaattcg aaaagccctg
      661 tcaagcgcca tcacaaggta ccgtggattg caaatatagg aatgggaaaa gaaatggaag
      721 ccgtaactat gtcccttcgc cccagccaaa caaagattgt ggccaccttg ggaccagcaa
      781 gtgattccta cgagaccatt tgtagcctca tccgctcagg ggttgacgtc tttcgtctta
      841 atgctgccca tgggagcctc gacgagcacg gagagcggct gcgtcgtatt cgccaagcag
      901 agcgggagat tggttggccg gtcggcgtcc tggtcgatct tggcggtcca aagatccggc
      961 tgggcgaact tgtcggcggg tcgctggaat gcacgccggg catgaaggtt cgcttcgtca
     1021 agaatgacaa ggccgagcgt ctgtccgatt tgacaagcac gtatccttcc
//
LOCUS       DAOPTR010001240         1068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_108313_length_1068_cov_3.804541, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001240 DAOPTR010000000
VERSION     DAOPTR010001240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108313_length_1068_cov_3.804541"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="PKI05_17060"
     CDS             <1..710
                     /locus_tag="PKI05_17060"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOA53966.1"
                     /translation="PSLGQRFIRIAEIFQSRQGEGLLTGTESVFVRTSGCNLRCWFCD
                     TPYASWAIEGDRLTIDEIISRVTSFSARHVVITGGEPMLFPGVVDLTHRLESKGYHIT
                     IETSGTRYLPVACHLLSISPKLSNSTPSWKEHPRWAAAHERRRYCPSVVRELIRRYPY
                     QIKFVIDTERDVAEVENYLEEVPEIDRHRVLLMPQGIDQRVLEEKARWLKPLCEKHGF
                     LFCPRKQIEWYGPVRGT"
BASE COUNT          207 a          301 c          317 g          243 t
ORIGIN      
        1 tcccatcgtt gggacagcgg tttatccgca ttgccgagat tttccagtct cggcaagggg
       61 aagggctatt gacgggtaca gagagtgtgt ttgtccgcac ctctgggtgt aaccttcgct
      121 gttggttttg cgatacgccc tacgccagtt gggccattga aggcgatcgt ctgaccatcg
      181 atgagatcat ctcccgcgtg accagttttt cggcgcgcca cgttgtcatt accggtggcg
      241 agccgatgct tttcccaggt gtcgtcgatt tgacacatcg tctcgagagt aagggctacc
      301 acattacgat tgagacttcg gggacgcggt atctgccggt tgcctgtcat ctcctctcga
      361 tcagccccaa gctcagcaat tcgacgccct cctggaagga gcatccccgc tgggccgctg
      421 cccacgagcg ccgccgctac tgcccatcgg ttgtccggga acttatccgc cgttaccctt
      481 accagatcaa atttgtcatc gacacggaac gcgatgtggc cgaggtcgag aattacctgg
      541 aggaagtgcc ggaaatcgac cgccaccgcg tcctcctcat gccccagggg atcgaccagc
      601 gagtcttaga agagaaggcg cgttggctga agccgctttg cgagaaacat gggtttttat
      661 tttgtccgcg aaaacaaatc gagtggtatg ggccggtccg cgggacatga cattgtcgtt
      721 cgcaattctc ctggtgcctg ccatcggccg atgcgcctga tgtgacagca ccgatagcac
      781 cgcaatcata tcggagccgg aggtaaatgc ccgacttcaa tgtcagttaa tacggccagg
      841 atgggggatt ggcacggctg cgccttgggg tagaataagc cgttggcatc gcggttttgc
      901 accttgtgtg ctgggatctc tcactcgtca ccacgtggct cgggggaaga aaggagtcga
      961 tccatgacga ctctctcgct cggttgggac ctcgacgtga cccaggggga aggttggttg
     1021 atcattcggc cgcggccaca cggaccacag acgctggaac tggtccct
//
LOCUS       DAOPTR010001241         1066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_108655_length_1066_cov_3.161227, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001241 DAOPTR010000000
VERSION     DAOPTR010001241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108655_length_1066_cov_3.161227"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PKI05_17065"
     CDS             complement(<1..625)
                     /locus_tag="PKI05_17065"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53967.1"
                     /translation="MATVPAASTRGNYWEESRAPLASLIFVTPLLLVYEAGVLLMGPE
                     AVRNGAEVWLRHLLMNLGLGQYFFLPVLTVGILLAAHHVSRHRWLVRSSTLWGMFVES
                     LFLAGCLRVLLGLQSLLFQHVSRYVLATGGSGVDFTLAERIVAFVGAGVYEELVFRLI
                     LMNLVIIVLQFLRLAPPVTLWGGVLLSSFVFALAHHIGPYGEPLQLFN"
BASE COUNT          280 a          294 c          280 g          212 t
ORIGIN      
        1 aattaaacaa ttggaggggt tctccataag gaccgatatg gtgggccaac gcaaacacga
       61 aactgcttag caacacgcct ccccacagcg tgacgggcgg cgccaatctc aaaaactgca
      121 gcacgatgat cactagattc attaagatga gccgaaagac caactcctca taaacaccgg
      181 ccccgacaaa ggcaacgatt cgctcggcaa gagtgaaatc gacgccgctt ccgcccgttg
      241 caaggacata tcgagaaaca tgttgaaaca gcaggctttg taggcccaga aggaccctca
      301 agcatccagc caaaaacaac gattccacga acatccccca gagggttgag gagcggacta
      361 accagcggtg gcgggaaacg tggtgggcag ccaagagaat ccccacagtc aagaccggca
      421 aaaaaaagta ctgccccaag ccaagattca tgagcaggtg gcggagccag acctcggccc
      481 cattgcgcac ggcctccggt cccatgagga ggacaccggc ctcgtaaacc aggagcagcg
      541 gtgtgacaaa tatcaaactc gccagggggg cccgtgactc ctcccagtag ttgcctcgcg
      601 tcgatgcagc aggtacggtc gccatagttt gaaaatatgt gactgcccta ataggccgac
      661 ttgatcgcca ctcgtttgta cgaacgctca aggtccgaaa gatatgcggg ccgcgaaaat
      721 gctgacactc ccggcggctt cccgtgagtc ttattatgtt gacttcgaca aaaggggtga
      781 gcaacgttca cggacaactc ttccgagcgt cgtccgtatt atcgcgtcct gttgttacaa
      841 ttggcgtaaa ccgctcaatc gacataaatc cttgccgggg acggtccgcc gttcgtacaa
      901 tcaacgagag tcgaggccgc tttaggttcg aggtactttc ggtttgtggt cgtgtcactt
      961 catctcactt gctgcatgct taatcgcgtg caatgtagaa aatgtcaagg tgacgtattc
     1021 tgttagccct gccgcggcat gttgatccag agaagtttta gttcgc
//
LOCUS       DAOPTR010001242         1062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_109305_length_1062_cov_3.102284, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001242 DAOPTR010000000
VERSION     DAOPTR010001242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109305_length_1062_cov_3.102284"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="PKI05_17070"
     CDS             complement(<1..837)
                     /locus_tag="PKI05_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOA53968.1"
                     /translation="MSDPYFQKLFAERIGGTQYGKGTEIYKFEKIKRAKRRVLAEHPE
                     RPLIDFGIGENDEMADESVRAVMAQEINKLENRGYADNGIPAFKEAVARFMEREFGVK
                     LDPTSEINHCIGSKTALAMLPAAFINPGDITLMTVPGYPVAGTHTRYYGGQVYKLPLL
                     PENDFYPDLDSIPTDVLRRTKLLVLNYPNSPTGQVATREFYRRVIDFALTHEIVVIQD
                     AAHLMLSYRDRPLSFLEVPGAKEVGVEVHSLSKGWHMIGWRLGWVCGNPRIVQAFADI
                     KDN"
     gene            complement(858..>1062)
                     /locus_tag="PKI05_17075"
     CDS             complement(858..>1062)
                     /locus_tag="PKI05_17075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53969.1"
                     /translation="KGELIQHVCRFSSLRDLGIHSDLTSDIRPKSGTSAIPCRDVRKR
                     DNKFLREKNGRKEVSLDRAPVVC"
BASE COUNT          250 a          290 c          262 g          260 t
ORIGIN      
        1 attatctttg atgtcggcaa atgcctggac aatgcgcggg ttaccacaga cccaacccaa
       61 cctccacccg atcatgtgcc aacctttgga gagcgaatgg acctcgaccc ccacctcctt
      121 ggcaccaggg acttcgagga aactcagcgg ccgatcgcgg taactaagca taagatgcgc
      181 cgcatcctgg atcacgacaa tttcatgggt cagcgcaaaa tcaatgacgc ggcgatagaa
      241 ctcccgtgtc gccacttgtc ccgtcgggct gttgggatag tttaggacca acaatttggt
      301 acgtctcaac acatccgtgg gaatggagtc caagtcagga taaaaatcat tttccggcaa
      361 aaggggtaat ttatagactt gcccgccata ataccgggtg tgcgtcccgg ccacaggata
      421 gcccggcacg gtcattaagg tgatgtctcc cgggttgatg aaagccgcgg gaagcattgc
      481 caaagcggtc ttggatccga tgcaatgatt gatttcggac gttggatcca acttgacccc
      541 aaactcgcgt tccatgaatc gcgcaacggc ttccttaaag gcgggaatac cgttgtcggc
      601 atatcctcgg ttttcaagtt tgttgatctc ctgtgccatg acggcgcgaa cgctttcgtc
      661 ggccatctca tcgttttcac cgatgccgaa gtcgatcagc ggcctttcgg ggtgctcggc
      721 caagacacgc cgttttgccc tcttgatctt ctcgaacttg tagatctctg taccttttcc
      781 gtactgggtg cctccaatcc gctcagcaaa gagtttttgg aaatagggat cgctcatgga
      841 actagacacc tgactgctca acaaaccaca ggagctctgt ctaaagaaac ttcttttcga
      901 ccatttttct ctcgcaaaaa tttattgtcg cgtttccgca catctctgca aggaatagca
      961 cttgtgcccg atttgggacg gatatccgaa gttaaatcgg agtgtatccc gagatcacgc
     1021 aggcttgaga aacggcacac gtgctgaatc agttcgccct tg
//
LOCUS       DAOPTR010001243         1062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_109365_length_1062_cov_2.075472, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001243 DAOPTR010000000
VERSION     DAOPTR010001243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109365_length_1062_cov_2.075472"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PKI05_17080"
     CDS             <1..574
                     /locus_tag="PKI05_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD+ synthetase"
                     /protein_id="HOA53970.1"
                     /translation="KKALLLTTSNRSEAAVGYATMDGDTCGCLSPIAGINKSYLREWL
                     KWLEKIGPEGLHPIPALAKVNALTPTAELRPAQEQQTDESDLMPYVVLDFVEKLAIRD
                     KMSPLDIYRLARNEFPEYSSQQLAAWIERFFQLWCQNQWKRERYAPAFHVDDEDLDPR
                     SWCRFPILSGGYQRELAQLRQYVTETGADH"
     gene            590..>1062
                     /locus_tag="PKI05_17085"
     CDS             590..>1062
                     /locus_tag="PKI05_17085"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOA53971.1"
                     /translation="MPFVYEYPRPAVCVDCVIFGWERPTRGRLMKVDVLLIRRGRPPF
                     EGTWALPGGFVEIEEPLLDAAFRELYEETGIRPKSLELAGVYGAPGRDPRGRTISIVY
                     RGLIWKDEHTVLNGDDASQAGWFSLGELPPLAFDHDQIIADVWQGLQHCASTQPFG"
BASE COUNT          221 a          277 c          318 g          246 t
ORIGIN      
        1 caagaaggct ttactcttga caaccagcaa tcgttccgaa gcggccgtgg gttacgccac
       61 catggatggc gatacctgtg gctgtctcag ccccatcgcc gggatcaata agtcctatct
      121 gcgggaatgg ctgaaatggt tggaaaagat cgggccggaa gggctgcatc ctattccggc
      181 gctggcaaag gtcaatgcat taactcccac ggcggaattg cgtcctgccc aagagcaaca
      241 gaccgacgag tcagacctga tgccctatgt ggtgttggat tttgtcgaga agctggccat
      301 tcgggacaaa atgtcgccct tggatattta tcgtttggcc agaaatgagt ttcccgagta
      361 ttcgtcgcaa cagcttgctg cctggattga gaggttcttt caactttggt gccaaaatca
      421 gtggaaacgg gagcgctatg cacccgcctt tcatgtcgat gatgaggacc ttgacccgcg
      481 aagttggtgc cgttttccga tcctctcggg cggctaccaa cgggagctcg cccagttgcg
      541 acagtacgtt acagaaaccg gcgctgacca ctgaggggga gcatcgcgca tgccgtttgt
      601 ttatgagtat cctcgacccg ctgtgtgcgt tgattgcgtg atcttcggct gggaacggcc
      661 tacccgcggt cgtttgatga aggtggatgt cctcttaatt agacggggaa gaccgccttt
      721 tgaagggacc tgggcattgc cggggggatt tgtcgagatt gaggaaccgc tcctggatgc
      781 agccttccga gaactgtacg aggagacagg aattcgcccc aaatcccttg aactggctgg
      841 cgtgtacggt gcgccagggc gtgatcctcg tgggcggacg atttcgatcg tctatcgtgg
      901 gctaatttgg aaagacgagc acaccgtgct taacggggac gacgcctccc aagccggatg
      961 gttttctctg ggcgagctac cccccttggc gtttgaccat gaccagatca tcgccgacgt
     1021 gtggcaggga ttgcaacact gcgcttcaac acaaccgttt gg
//
LOCUS       DAOPTR010001244         1061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_109451_length_1061_cov_4.104374, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001244 DAOPTR010000000
VERSION     DAOPTR010001244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109451_length_1061_cov_4.104374"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="PKI05_17090"
     CDS             <1..945
                     /locus_tag="PKI05_17090"
                     /inference="COORDINATES: protein motif:HMM:NF014860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOA53972.1"
                     /translation="ILCVGVRPEVTLAENAGLKIGELGGIRVDEQMRTSDPSIFAVGD
                     AVEVKDLVTGKWTWVPLAGVASRQARVAADAICGRPSQFRGALGTAIVTAFGVTIAKT
                     GLTEKAAQRDGVRYQKSYSHSPHHATYYPGAKSIAIKLLFDPQDGRILGAQAVGETGV
                     DKRIDIIAMAIQKGGTIFDLEEAELCYAPQFGSARDPINIAGFVAGNVCRGDADLAYW
                     EDLDHKPQADAPLVVDVRTPGEHAAGAPPRAINIPLGELRSRLHELPTDRELWVHCGI
                     GQRAYYAARILKQKGFRVKNLSGGYVTYRMYRRSKPAD"
BASE COUNT          230 a          283 c          333 g          215 t
ORIGIN      
        1 attctttgtg tgggagttcg cccagaggtg acgctggcag agaacgccgg attgaagatc
       61 ggcgagttgg gtgggatccg cgttgatgag caaatgcgaa caagcgatcc gagcattttt
      121 gctgtcggtg acgccgttga agtcaaagac ctggtcacag gcaagtggac atgggttccc
      181 ctggcgggag tggcctcgcg acaggcgcgg gtggccgccg acgccatctg cggtcgcccc
      241 agccaattcc gtggtgcctt ggggactgcg attgtgacgg cctttggggt cacaatcgcc
      301 aagacgggat tgaccgaaaa agcggcccag cgagatggtg tccgttatca gaagagctac
      361 tcgcattcac cgcatcacgc cacgtactat ccgggagcca agtcgatcgc gattaagtta
      421 ctttttgatc ctcaagatgg tcgtatcctt ggcgctcagg ccgtcgggga gaccggtgtt
      481 gataaacgga ttgacatcat cgcaatggcc attcagaaag ggggtaccat ctttgatttg
      541 gaggaagccg aactgtgtta tgccccccag tttgggagtg cccgcgaccc catcaacatt
      601 gccggatttg tggctgggaa tgtctgccgg ggagacgcgg acctcgctta ttgggaagac
      661 ctggatcaca agcctcaagc ggatgcgcca cttgtcgttg atgtgaggac accgggagag
      721 cacgcagcgg gggccccacc tcgggcgatc aatatccctc tcggagagct tcgcagtcgc
      781 ttgcacgaat tgccgacgga tcgtgaattg tgggtgcatt gcggaattgg tcaacgggcg
      841 tattatgccg cgaggatcct gaagcaaaaa ggtttccgtg ttaagaacct atcgggaggc
      901 tacgtgacct accgcatgta ccgccgcagc aagcccgccg attaagaatc gatcccaaaa
      961 agtcccccct ctccctccgg gagagggttg gggtgagggc aagcgaacgt cggaatcgat
     1021 tgagaaaacc ggcagcgatt cgagccaccc tcacccccga c
//
LOCUS       DAOPTR010001245         1061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_109470_length_1061_cov_2.979125, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001245 DAOPTR010000000
VERSION     DAOPTR010001245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109470_length_1061_cov_2.979125"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PKI05_17095"
     CDS             <1..845
                     /locus_tag="PKI05_17095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53973.1"
                     /translation="AHYTQFMVYDLDGDGKAVEIHELKDPRILEIQKAYVRHVIDTVN
                     EFDNVLYEISNENHPPSTDWQYEMIRFIKAYESEKGRQHPVGMTFQYRGGSNKTLFDS
                     PADWISPNPEGGYRDDPPAADGSKVIINDTDHLWGIGGNRTWVWKSFMRGMNPIFMDP
                     YDGRILSRGSNLAWTEDVRKAMGDVRKWSQRVDLTSMTPRPDLASTKYCLANPGKEYL
                     VFFPEKTDGTEIQLVPGRYELRWFDTRARQEQPNGTLATSKESMFVKVPADGEWLLHV
                     KAVQ"
BASE COUNT          253 a          253 c          310 g          245 t
ORIGIN      
        1 gagcgcatta tacccagttc atggtctatg atctcgatgg cgatggcaaa gccgtggaaa
       61 tccacgaatt gaaagatccc cgaatcctcg agattcaaaa ggcctatgtt cgtcatgtca
      121 tcgatactgt caacgagttt gataacgttc tgtacgaaat ttccaatgag aatcatcccc
      181 cttcaaccga ctggcaatat gagatgatcc gattcatcaa ggcctatgag agcgagaaag
      241 gccggcaaca cccggtgggg atgacgtttc aataccgcgg tggcagtaat aaaacgctat
      301 tcgacagtcc ggcagactgg atttcgccga atccagaagg ggggtatcga gatgacccgc
      361 cggctgccga cggttccaag gtcattatta acgacacgga tcacctttgg gggattggtg
      421 ggaatcgcac atgggtttgg aaatccttca tgcgcggtat gaatcccatc tttatggatc
      481 cctacgatgg gcgaattctt tcgcggggat caaacttggc ctggacggaa gatgtgcgaa
      541 aggccatggg tgacgtgcgg aaatggtcgc aacgtgttga cctgacgagc atgacgccac
      601 ggccggacct cgcgtcgacc aaatactgtc tagcgaatcc tggcaaagag tacctggttt
      661 tcttcccgga gaagaccgac ggtacggaga tccagttggt gcccggtcgt tacgagcttc
      721 gctggtttga cacccgtgct cggcaagagc aacctaacgg aactcttgcc acgtcgaagg
      781 aatccatgtt tgtcaaagtc ccggccgatg gagaatggct cctccacgtg aaggcggtgc
      841 agtgaacaag cgttgtgtcc caatcaggcg gctcacgatg acgccgtccg tttggtgagc
      901 aagagatttt ggtcattagg cctattaggg gaggattttc acatgagggt gtttatgtcg
      961 cgttggagcg gctccacggc gctattgggg cttgtgggaa tcgggttatc tttgctacat
     1021 gggagcacgg gatgggcaag tgaggtaaat cgccccaatt t
//
LOCUS       DAOPTR010001246         1061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_109532_length_1061_cov_2.018887, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001246 DAOPTR010000000
VERSION     DAOPTR010001246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109532_length_1061_cov_2.018887"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..461
                     /locus_tag="PKI05_17100"
     CDS             219..461
                     /locus_tag="PKI05_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53974.1"
                     /translation="MAYGYFIQGCPTCGRKVQIRLDYLGKYVTCHHCGAAFLAFGENR
                     PEKGDGNRPVPGHTIALKKTWQSEHMAVWPFGPEET"
     gene            complement(611..>1061)
                     /locus_tag="PKI05_17105"
     CDS             complement(611..>1061)
                     /locus_tag="PKI05_17105"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660272.1"
                     /note="E3 component of pyruvate and 2-oxoglutarate
                     dehydrogenase complex; catalyzes the oxidation of
                     dihydrolipoamide to lipoamide; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOA53975.1"
                     /translation="AHEGKTAVEAISGHKVAFEPRAIPAVVFTDPEIAWTGLTETDAK
                     RKGIPYEVAVFPWAASRRAQAMGRTDGFTKWIIDPKTERVMGCGIVGPSAGDLIGEAT
                     LAVEMAASARDLAETIHPHPTLCETLGFAAEVFFGQATDLYRPRKKD"
BASE COUNT          216 a          279 c          314 g          252 t
ORIGIN      
        1 cgacaccgcg tggctcgctc acccggcctg tggttcccta tcacagcgcg tggccgcgtt
       61 ccggtcgttt ggctgttcgt ggataagatt ggccctacgt cgaagcgaag gactttagtt
      121 aaattgctgt ccgagatact tgccagacct atcctgagcg agtagtatac cggttgtgag
      181 gatcgtacca gcaaggaaat ttcgaggagg tgcgacggat ggcgtacgga tacttcattc
      241 agggatgtcc gacatgtggt cggaaggtcc aaattcggct cgattacttg ggaaaatacg
      301 ttacttgtca ccattgtggt gccgcattct tggcttttgg cgagaatcga ccggaaaagg
      361 gtgatgggaa tcgaccagtg ccgggccaca ccattgcttt aaagaagacg tggcaaagcg
      421 agcatatggc cgtgtggcct tttggtccgg aagaaacctg atccagcgtt tatgccacca
      481 ggtttttcgc cacggtcgaa gtgcgcatgg gttgcgctgt ttggggcggg gagatttttt
      541 ggggagcgat ggggcagccg tggctaattt gctgcgaaat gtgggcccga gtaaaaattg
      601 caaatctgcc ttaatccttt ttgcgtggcc gatagaggtc ggtcgcttgt ccgaaaaaga
      661 cctcagccgc aaatcctaag gtctcgcaga gtgtgggatg ggggtggatc gtctccgcaa
      721 ggtctcgagc cgaagcggcc atttcaaccg ccaacgtcgc ttccccgatt aggtcgccgg
      781 cgctgggccc aacgatccca cagcccatga cgcgctccgt cttggggtca ataatccact
      841 tggtgaaccc atctgttctt cccattgcct gcgcccggcg acttgctgcc caaggaaata
      901 cggcaacctc atacggaatc cccttgcgtt tggcatctgt ctctgtcaat ccggtccagg
      961 caatttcggg atcggtgaaa accacggcgg ggatagccct tggttcaaag gccactttgt
     1021 gcccagaaat ggcctcgacg gctgtcttac cctcatgggc c
//
LOCUS       DAOPTR010001247         1057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_110109_length_1057_cov_2.512974, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001247 DAOPTR010000000
VERSION     DAOPTR010001247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110109_length_1057_cov_2.512974"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            417..>1057
                     /locus_tag="PKI05_17110"
     CDS             417..>1057
                     /locus_tag="PKI05_17110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53976.1"
                     /translation="MRRYGRFLFVVCFALSVVMTNIRAAVAIEPPMAAKLFPAKTAVF
                     FSIPDVPELKRAVLASSSGQMLQDPQLRPFLTDLWGGIVEAAAVVEKETGVSLEAILA
                     IPKGELSVGLVSSEKQGWVVLIFMDAKENIETLKKLIDRGREAAVKAGAILKEEDVNG
                     LPLTVLRPRERQGNEDNRRPQPLVAYAINGSTLIAGLAVLGEEDLDVIRDVVG"
BASE COUNT          233 a          253 c          336 g          235 t
ORIGIN      
        1 gtattctcca ccgcgacgaa ttgcgaaaat ggacgtggct ttgggatccc cgactaggcg
       61 gaatcgaggt gaagaacaaa aacgacgaga taacggtgga gtacgagttc cccaacaccg
      121 ctgagcgtgg cctgaaggga ctgtatcctt attgatgtag gtgaccggcg gaacacacca
      181 cccagaagaa cgagagcggc tggtaccgct ctcgatgtgt aaccttgccg gatttcttgt
      241 gtagatagag ttggcaaggg gcctcgtacg gcccaatatc gccattgacc taataagctg
      301 gctcgacaat gccgccagtg tgcgggttct ggcgttcctg ggttgaatag ctagcggccg
      361 aggctcgccg ggcatcgtgg aaacgtagtg actacttccc aagaggagaa ctgattatgc
      421 ggaggtatgg ccgattcctg ttcgttgtgt gtttcgctct tagtgtggtc atgacgaaca
      481 taagggccgc ggtggccatt gagcctccga tggcggcaaa actctttccc gcaaaaacgg
      541 ccgttttctt ttccatacct gacgtgcctg agttgaaaag ggcggtcctc gcgtcttcgt
      601 ctgggcagat gttacaggat ccacagttgc gacccttctt gacggatttg tggggaggga
      661 ttgtcgaggc cgccgcagtg gtcgagaaag agactggtgt ctccttggag gcgatcctcg
      721 cgattcccaa gggtgaatta tccgtgggct tggtatcgtc tgaaaagcaa ggctgggttg
      781 tgttaatctt tatggatgcc aaggagaaca ttgaaacgct caagaagctc atcgaccgcg
      841 ggcgagaggc cgcagtgaag gccggagcca ttctcaagga ggaggacgtc aatggtttgc
      901 ccctcacggt gctccggccg agagagcgcc aaggaaatga ggataatcga cggccgcagc
      961 cgcttgtggc ctacgccatt aacgggtcaa cgctcattgc agggctagca gttctggggg
     1021 aggaggattt ggacgtcatc cgtgacgttg ttgggag
//
LOCUS       DAOPTR010001248         1055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_110446_length_1055_cov_2.415000, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001248 DAOPTR010000000
VERSION     DAOPTR010001248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110446_length_1055_cov_2.415000"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..477
                     /locus_tag="PKI05_17115"
     CDS             202..477
                     /locus_tag="PKI05_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53977.1"
                     /translation="MVSSQMDTIPCLEELKPGDRVEVEHQVTVGGKSWTTRTVGTVVR
                     TERVCHGLHFERAPDERTYSDMILLELPDGELTAVTVDEFTVIRRAP"
     gene            652..>1055
                     /locus_tag="PKI05_17120"
     CDS             652..>1055
                     /locus_tag="PKI05_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOA53978.1"
                     /translation="MSDRASVFLQHSRNFGEFRFIYPVVSRRARGVSVGVNLNPDKVC
                     NFDCVYCQVDRRSPGNKAFGEMEQLRAELLAAIQLVQTGALFEHPTFAATPLPLRRLN
                     DIAFSGDGEPTTFANFDAVIQLAADIRRAESL"
BASE COUNT          199 a          266 c          331 g          259 t
ORIGIN      
        1 tccccatcga gcggtaggtc attccttccg aaatgtcgtt ccgcgtactg gactgccttg
       61 cgcgtgcaat ttttgacaag tattagtgtt gttccgaaaa ccgcccgatt gtcgagtacc
      121 cttgtccccc gcttgtggcg ggccatatga ttatcttacg ggagcaaggg ttttcggggt
      181 ctgagtcggg aggtcgttaa catggtcagc agccagatgg acacaattcc gtgtttggag
      241 gagctcaaac caggtgaccg cgtggaggtc gagcaccagg taaccgtcgg cggtaaaagc
      301 tggacgacgc ggacggtggg aaccgtggta cgaaccgagc gggtgtgcca cggtctgcac
      361 ttcgagcggg cacccgatga gcgaacgtac agcgatatga tcctcctgga attgcccgac
      421 ggcgaattga cggccgtgac ggtggatgag tttaccgtta ttcgcagggc accttgagga
      481 gggctctgat cctgtgctga catggaatct cggggcggag ggttggattt ctagctggcc
      541 tgttgggacg ggcgatcttc cgtaatggac gaggtgggca gtcgcgatgg ggtttcctcg
      601 ccttcgttgt tgatctgtct tgttgaattt ctttgctaaa gggaatctgc gttgagcgat
      661 cgggcctcag tttttttgca acacagccga aactttggcg agtttcgctt tatttatccg
      721 gtcgtcagcc gccgcgctcg gggtgtgtcg gtgggtgtga atctcaatcc cgacaaggtg
      781 tgtaacttcg attgcgttta ttgccaagtc gatcgccgaa gccccgggaa caaggccttt
      841 ggggaaatgg agcaactgag agccgagctt ctggcggcca tccagttggt tcagacgggg
      901 gcgctctttg aacatccgac atttgcagct acccccctgc cccttcgacg gctcaacgac
      961 atcgctttca gcggtgatgg ggaaccaacg acgttcgcca attttgatgc cgtgattcaa
     1021 cttgcggcgg acatccgtcg tgccgagtca ctgga
//
LOCUS       DAOPTR010001249         1055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_110457_length_1055_cov_2.182000, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001249 DAOPTR010000000
VERSION     DAOPTR010001249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110457_length_1055_cov_2.182000"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PKI05_17125"
     CDS             <1..382
                     /locus_tag="PKI05_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53979.1"
                     /translation="YTYQGNHNQLVADVLGVPPAFERLLEVPSARVVHYEPLLTLALD
                     QDCNLQCRVNIETRTQDYHVRKEEYPEEQISVYVTCRHYGLLPGDNRFLRTLDLLLKT
                     GMTTVDDFVAPMILEPLAKAIALR"
     gene            537..>1055
                     /locus_tag="PKI05_17130"
     CDS             537..>1055
                     /locus_tag="PKI05_17130"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOA53980.1"
                     /translation="MLPRRNFVLVLGVLAWLGLPNWGLAASEVPYKDRTVILVSIDGL
                     ANFYLDDPRADIPTIRRLAAEGARADAMVTVFPSVTWCAHATLSTGVWPNRHGVLAND
                     YIDRNTKQKVTLLCDPVFDKDQILQTPTIYDVAHAAGLVTAGVLWPVTRNARTLHFSA
                     PDMPGDTAWSDFG"
BASE COUNT          217 a          305 c          286 g          247 t
ORIGIN      
        1 ctacacctat cagggtaatc acaatcagct tgttgccgat gtcctcggcg taccacctgc
       61 ctttgagagg ctcctcgaag taccgtccgc gcgagttgtc cattacgaac cattgttgac
      121 cctcgccctc gatcaagact gtaacctgca atgccgcgtc aatatcgaaa cgcggacgca
      181 agactaccat gtcagaaaag aggaataccc ggaagagcag atcagcgtct atgtgacgtg
      241 tcgtcattac gggctcttac ctggggacaa ccgtttcctg cggacattgg atctccttct
      301 gaagacaggg atgaccacgg tggatgattt cgtcgcccca atgatcttag agccgttggc
      361 caaggccatc gcgttgcggt aatctgcgtt ctggtcttcc agtacgaagc ggcaacctct
      421 gctttggatg tcctcttccc gggaaaggtt tgcctgctgg ggcgaaaggc attagaatcc
      481 tttcgattgg gccaattttt gtgactgaca ttttacctag ctagaggagg acgaccgtgc
      541 tgcctcgtcg caacttcgtg cttgttttgg gggtgttggc ctggctgggg ctacccaatt
      601 ggggtctggc cgcaagtgag gtcccttaca aggatcgcac tgttattttg gtgagcattg
      661 atggcttagc caacttctat ctcgacgatc cccgagcgga cattccaacg atccgtcgtt
      721 tggccgctga aggagcacga gccgacgcga tggtcacagt gttcccctcg gttacgtggt
      781 gtgcccacgc cacgctctcc accggggttt ggcctaaccg tcatggggtc ctggccaacg
      841 actacattga ccggaacacg aagcaaaagg taaccctgct ttgcgacccg gtcttcgaca
      901 aagaccaaat cttgcaaaca cccaccattt acgacgtcgc ccacgctgcg ggattggtga
      961 ccgccggcgt gctctggccc gtcacgcgaa atgcccgaac actgcatttc agcgctccag
     1021 acatgcccgg cgacacggcg tggtccgact ttggt
//
LOCUS       DAOPTR010001250         1051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_111011_length_1051_cov_3.697791, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001250 DAOPTR010000000
VERSION     DAOPTR010001250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111011_length_1051_cov_3.697791"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..>1051
                     /locus_tag="PKI05_17135"
     CDS             259..>1051
                     /locus_tag="PKI05_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOA53981.1"
                     /translation="MRRSCHSAKAFTLVELLVVIAIIGILIALLLPAIQAAREAARRS
                     QCSNNLKQIGLAIHNFQDVYGRLPTGARDGDHRVPDALTACCNSRTRYGWSFWYHILP
                     FIEQRGLYDLATDADDPPTPGSGQNSKEDIVAQKFVTTYYCPSRRLPEPYGSGKFYRS
                     DYAGNAGQRGEGDIRDTPSGGLRGPIIQTDRNRTRIELIRDGSSNTILVAEKALHPKS
                     FGSDGGDNERWNNAGWDEDIIRFGAGRLNNGTEYGLPPIPDNTAPY"
BASE COUNT          235 a          282 c          282 g          252 t
ORIGIN      
        1 tccatgaccg ctgttcgtca ttgtttaatt ctctcgtacg cccaccgagc aagacgcaac
       61 gatgtttacg cgcgattcca aagtgcttcc acccgtcttt tcctaacaca ccgcgaatac
      121 ccatttgtgg gggatacaaa tgacttcaca cccgagttga caaaatgttg acaacttacg
      181 ttgcgaggaa taaaatacgc cagcgttggg atgtggggaa tgtctcttgt cacttttcct
      241 ttgttggagg tgaggacgat gcgacgctct tgccattctg ctaaggcatt tactcttgtt
      301 gagctgttgg tggtgatcgc catcatcggc attttgattg cgcttctctt accggcgatt
      361 caggcggcac gagaagctgc tcgcagatcg cagtgctcga acaatctcaa gcagatcggc
      421 ttagccatcc acaattttca ggatgtgtat gggcggcttc ccacgggtgc ccgcgatggt
      481 gaccaccgcg tccccgacgc tctaactgcg tgctgcaatt cgcggacgcg ttacggctgg
      541 agtttttggt atcacatcct gccgtttatc gaacagcggg gactctatga tttggcgacc
      601 gatgcggatg atcccccgac gccgggatct ggccaaaact ccaaagagga tatcgtggcc
      661 caaaagtttg tgaccaccta ttattgccct tcgcgacgcc ttcccgaacc ctacggtagc
      721 ggaaagtttt accggtcgga ctatgccggg aatgctggac agcgaggcga aggcgatatc
      781 cgggacacgc caagcggtgg tctgcgtggc ccgattattc agaccgatcg caataggacg
      841 cggatcgaac taatcaggga tggttcttcc aacacgattt tggtggctga aaaggctctc
      901 catcctaaat catttgggtc ggatggtggc gacaacgaac gttggaacaa cgccggctgg
      961 gatgaagaca ttattcgctt tggcgccgga cgacttaaca acggcaccga atatgggctt
     1021 ccgccaatcc cagataacac ggcgccgtac a
//
LOCUS       DAOPTR010001251         1049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_111313_length_1049_cov_5.082495, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001251 DAOPTR010000000
VERSION     DAOPTR010001251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111313_length_1049_cov_5.082495"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PKI05_17140"
     CDS             <1..514
                     /locus_tag="PKI05_17140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOA53982.1"
                     /translation="AFWRHGRNSFPIVGVDFCREMVQLAKRKADRRHAQIHFIEADGL
                     HLPFPDDTFAVVAVAFGLRNMADTGRGLSEMVRVCRPGGEVMVLEFTLPTLFPINVIF
                     RAYFRFLLPIIGQALAPNRQEAYNYLPASVHEFPQGEALLAEMAKAGLKDLVLRPFSF
                     GIATLYKGSK"
     gene            511..>1049
                     /locus_tag="PKI05_17145"
     CDS             511..>1049
                     /locus_tag="PKI05_17145"
                     /inference="COORDINATES: protein motif:HMM:NF014494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HOA53983.1"
                     /translation="MKRQIVVAITGASGVTYAIRLIEVLLAAACDVHLTISRAGRTVL
                     KEELDLTVDLDNFQPSSLLLDSQDSAEDTKLRLLRGMAGVAGESSHVLGVPIGEPGTL
                     YYHHFKRLTAPIASGSFITHGMVICPCSMSTLSTIVHGGGNNLIHRAAEVHLKEGRKL
                     ILVPRETPLSLIHLRNLALA"
BASE COUNT          222 a          259 c          293 g          275 t
ORIGIN      
        1 ggctttttgg cggcatggga gaaatagttt tccaatcgtg ggcgttgact tctgtcgaga
       61 aatggtgcaa ctagcaaagc gaaaggctga tcggcgtcac gcccaaatcc atttcatcga
      121 agcggatggc ctacatttac cctttccgga tgatactttt gccgttgtgg cggttgcctt
      181 tggactccgt aacatggccg acaccgggcg agggctttct gagatggtcc gagtctgtcg
      241 gcctggggga gaggtcatgg tgctggagtt tacgttaccg acgctcttcc caatcaacgt
      301 aatttttcga gcgtactttc gtttcttgct tccaatcatc ggtcaggctt tggctcccaa
      361 tcggcaggaa gcctataact accttccggc tagcgtccat gagtttcctc aaggggaagc
      421 tttattggct gagatggcca aggcgggact aaaagacttg gtgctaagac cgttctcatt
      481 cgggattgct acgttgtaca aaggatcaaa atgaaaaggc agattgttgt cgcaatcacc
      541 ggcgccagtg gcgtgactta cgccattcgt ctcatcgagg tacttcttgc ggctgcctgt
      601 gatgtgcact tgaccattag tcgggcgggt cggacggtgc tcaaggagga gctggacctc
      661 accgtcgatc tggataattt ccagccgtcg tctttacttc ttgacagtca agacagtgcc
      721 gaggacacca aacttcggct tcttcgtgga atggctgggg tagcaggaga atcgagtcac
      781 gtcttggggg tgcccatcgg tgagcctgga acgctttatt accatcattt caaacggctg
      841 acggccccca ttgccagcgg gtcatttatc acccatggga tggtgatttg tccgtgttcg
      901 atgagtacgc taagcaccat tgttcacggt ggtggtaata accttatcca ccgagcggcg
      961 gaggtccatc tcaaggaggg aagaaaactc atcctggtgc cccgcgaaac accattgtct
     1021 ttgattcatc ttcgaaactt ggcattggc
//
LOCUS       DAOPTR010001252         1048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_111496_length_1048_cov_3.015106, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001252 DAOPTR010000000
VERSION     DAOPTR010001252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111496_length_1048_cov_3.015106"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PKI05_17150"
     CDS             <1..704
                     /locus_tag="PKI05_17150"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOA53984.1"
                     /translation="RRLSVFLDRRQENTSTMAEQTSDSERHLISRVRSGDPEAWKEVI
                     RRYEGRLYSYLGSHVKHRDIAEELVQETFVGFLVSLPNYDSRRSLENWLFSIAAHKLT
                     DYYRRQGRRITLSLEEGIEGANRCAIGGRPQRGPSTVAASHERRGIEEQALSEAIEKQ
                     IMAWKSEGEWLKVSCMELLFLCGWSNKETAQYLHIDEQQVANWKFDFLRRIRERLRAQ
                     GLPQDVFPELQGEEK"
     gene            738..>1048
                     /locus_tag="PKI05_17155"
     CDS             738..>1048
                     /locus_tag="PKI05_17155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53985.1"
                     /translation="MAQERISDQELEAYLDEALPPERMVAIENALRHNHECLLRLHRI
                     VSRRDNGIHSVAEIWRRNRLSCPSREALGAYLLGALTEPESTYVKIHLETVGCAICLA
                     NL"
BASE COUNT          254 a          253 c          311 g          230 t
ORIGIN      
        1 accgccgctt atccgttttt ctcgacaggc gacaagaaaa caccagtacc atggcggaac
       61 aaacatcaga tagtgaacga catctgattt ctcgcgtccg gtcgggcgat cccgaggcgt
      121 ggaaagaggt tatccgccgc tacgagggcc gactctactc atatcttggg agtcatgtga
      181 aacaccgaga tatcgctgag gaattggttc aggagacttt tgtcgggttt ctggtgagcc
      241 tccccaacta tgacagtcgg cggtctttgg agaattggct gttttctatc gcggcccata
      301 agttgacgga ctactaccgg cggcaaggac ggcgcatcac tctgtcctta gaggagggaa
      361 tcgagggcgc gaatcgttgt gccattggtg gcaggcccca acgtggccct agcacggtcg
      421 cggccagtca cgaacgccga ggcatcgaag aacaggccct ttccgaggcc atcgaaaaac
      481 agatcatggc ttggaaaagc gagggagaat ggttgaaagt gtcgtgtatg gaactgctgt
      541 tcttgtgcgg gtggagtaac aaagaaacgg cccaatatct gcacatcgat gagcagcagg
      601 ttgccaattg gaaattcgat ttcttaaggc gcatacgaga gcgactacgg gcacaggggc
      661 ttcctcaaga tgtgtttccg gaacttcaag gggaagaaaa atagtcaacc tgaagccgaa
      721 agtaacgaaa gcgaacgatg gcccaggaaa gaatttcgga ccaagagctt gaagcctatt
      781 tggatgaggc gctgccccct gagcggatgg tggcgattga aaatgccctc cggcataacc
      841 acgagtgttt attgcgatta catcgcatcg tatctcggcg ggataatggt attcactcgg
      901 tggccgagat ttggcgtcgg aaccgcttga gttgcccctc gcgggaggct ctcggggcgt
      961 atcttctggg ggccctaact gagccggagt cgacatacgt gaaaattcat ctcgagaccg
     1021 ttggttgcgc tatctgcttg gctaatct
//
LOCUS       DAOPTR010001253         1047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_111667_length_1047_cov_2.891129, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001253 DAOPTR010000000
VERSION     DAOPTR010001253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111667_length_1047_cov_2.891129"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PKI05_17160"
     CDS             complement(<1..585)
                     /locus_tag="PKI05_17160"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOA53986.1"
                     /translation="MIKRILVTGGAGFLGSHLCERLVEMGHDVICLDNFFTSQKSNVA
                     HLLDRPNFELIRHDITLPIWLEVDEIYNLACPAAPGHYQYNPIKTMKTSLMGAINVLG
                     MAKRCRAKVLQASTSEVYGDPEIHPQPETYRGSVNPIGPRACYDEGKRAAEALFMDYH
                     RMNRVDIRIVRIFNTYGPRMHPYDGRVVSNFIRQA"
     gene            complement(703..>1047)
                     /locus_tag="PKI05_17165"
     CDS             complement(703..>1047)
                     /locus_tag="PKI05_17165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOA53987.1"
                     /translation="EAVQKGKQYDLLLMDVEMPVMDGPQAVRQLRAIGCQTPIVALTA
                     HTDPVCVQQFLQSGYTGLIPKPVDRDGLLEAVASILTPRGTKIPTLVQVNEQQWMDHM
                     GASGLVLPVVNQ"
BASE COUNT          220 a          282 c          299 g          246 t
ORIGIN      
        1 ggcttgacgg atgaagttgg aaacgacgcg gccatcatag gggtgcattc gtgggccata
       61 tgtgttgaaa atccgcacaa tgcggatatc gacccgattc atgcgatggt aatccataaa
      121 gagggcctcc gctgcccgct ttccctcgtc gtagcaggcg cggggaccaa tgggattgac
      181 ggatccgcgg taggtttccg gctggggatg gatttcggga tcgccataga cctcgctggt
      241 tgaggcttgt aacaccttgg cccgacaccg cttggccatg cccaggacgt tgatcgcccc
      301 catcagcgag gtcttcatcg tcttaatggg gttgtattgg taatgtccag gcgctgctgg
      361 gcacgccaaa ttgtagattt cgtccacctc caaccagatc ggcagcgtga tgtcgtgacg
      421 aatcaactca aagttgggtc ggtccagtag gtgggcgaca ttcgactttt gactggtaaa
      481 aaagttgtcc agacaaataa cgtcgtgccc catctcaacg aggcgttcac aaaggtggga
      541 accgagaaaa ccggcgccac cagttaccaa gattcgtttg atcatcgcca tcgcgtcatc
      601 ctctcgccgt gttctcaaaa ggcctttttg ctatcttggg cggagtggtt tcttctgtaa
      661 agccgagccg agataacaga agtggaatcc tttatcccgc tgtcattgat tgacaacggg
      721 taggacgagc cccgacgcac ccatgtggtc catccattgt tgctcgttga cctggaccaa
      781 tgtgggaatc tttgtgcctc tcggtgtgag gatactcgcc acggcctcca gcaggccatc
      841 ccgatccacc ggttttggga tcagccctgt gtagccggac tggagaaact gctggacgca
      901 caccgggtct gtgtgggcgg tcagggcgac aattggggtt tgacagccga tcgcgcgaag
      961 ctgccgaacc gcttgggggc catccataac cggcatttct acatccataa ggagcaaatc
     1021 gtattgtttg cctttctgca ccgcctc
//
LOCUS       DAOPTR010001254         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_111802_length_1046_cov_4.049445, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001254 DAOPTR010000000
VERSION     DAOPTR010001254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111802_length_1046_cov_4.049445"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PKI05_17170"
     CDS             <1..602
                     /locus_tag="PKI05_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911765.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOA53988.1"
                     /translation="RDIEQALDAVDGFVLIGGDDLDPLRDGCYHHPTTRVMHPRREDF
                     DRLLVKLIAERHMPVLGIGAGMQLLNVSQGGTLSLNIRDDFSNAMDHRDPHDPYHRHG
                     LVVSPKTFAKRVYGDGEIRVASRHHMAVLDLAPIFKVSAVAPDGIIEVIESQDPDWFA
                     IGVQYHPECEAASALDLRVFEEFVAEVVRRQSAMQRAVA"
     gene            complement(656..>1046)
                     /locus_tag="PKI05_17175"
     CDS             complement(656..>1046)
                     /locus_tag="PKI05_17175"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HOA53989.1"
                     /translation="LFAVELNVAGLPANADDRFFESLEGARLGHLVTQLDLEETPGLA
                     LVDSWLGLRLQLAFSRPTNLWTFPVASVSQSEGGFELVHQSVAIQPHWWVTPDATGRW
                     SVMMRFLADTSAASQRESVAELATASV"
BASE COUNT          222 a          312 c          288 g          224 t
ORIGIN      
        1 gccgtgatat tgaacaagcg ctggatgccg ttgatggctt cgtgcttatc ggcggcgacg
       61 atctggatcc tttacgcgat ggctgttatc accatcccac cacccgggtg atgcaccccc
      121 gccgggaaga ctttgaccgc cttctggtca agctcatcgc cgaacgtcat atgcccgttc
      181 tgggaatcgg tgctggcatg caactgctca acgtgtcaca gggtggaaca ctgtcattga
      241 atatccgcga cgacttctcc aatgccatgg atcatcggga tccacatgat ccctatcatc
      301 ggcacgggct tgttgttagc cctaagacct tcgcgaagcg tgtctacggc gatggtgaga
      361 ttcgagtcgc tagtcggcat cacatggccg tactcgactt ggctcccatc ttcaaagtgt
      421 cggccgtggc acccgacggg attatcgagg tcattgaaag ccaggacccg gattggttcg
      481 ccattggcgt tcaatatcac cccgagtgcg aggcggcttc cgcactggac ctgcgtgtct
      541 ttgaagaatt tgtggctgaa gtcgtgcgtc gccagagcgc catgcagcgg gcggttgcgt
      601 aaccatctcc tcgatgggaa ggtggcggaa aaagccaccc ctggctgctc cccgtttaca
      661 cactggctgt cgcaagctct gcaaccgact cccgttgaga agcagccgac gtatctgcaa
      721 gaaaccgcat catcaccgac cagcgccctg tggcgtcagg tgtcacccac cagtgcggtt
      781 gaattgctac cgactggtgg accagttcaa aaccgccttc ggattgactg actgacgcca
      841 cggggaacgt ccatagattt gtcgggcgag aaaaggccag ctggagtcgc aagcctaacc
      901 aactatccac taaggccaag cctggggttt cctcaaggtc caactgggtc accagatgcc
      961 cgagtctcgc tccctccagg ctttcaaaga aacggtcatc ggcattggca ggaagtcccg
     1021 ccacgttcaa ctccacggca aaaagt
//
LOCUS       DAOPTR010001255         1042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_112536_length_1042_cov_2.403242, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001255 DAOPTR010000000
VERSION     DAOPTR010001255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112536_length_1042_cov_2.403242"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1042
                     /locus_tag="PKI05_17180"
     CDS             <1..>1042
                     /locus_tag="PKI05_17180"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA53990.1"
                     /translation="CGGRGGGLAQAFAEISDIAYVCDPDRSRCERLQGAVKAKKAVTD
                     PRQVLDDRSVDAVVIATPDHWHAPLAILVCEVGKHVYVEKPLSHNFRESQLLVEAARR
                     ARVVVQHGTQARSSPLINKAIQLLHTGVIGDVLVAKAWNVQRRVNIGHEQPSEPPPGV
                     DYDMWVGPAEFVPFQKNRFHYTWHWWYNFGTGDIGNDGVHELDYGRWGLGVSGLPNHV
                     MAEGGKYFFDDDQEFPDTVTCAFEWTGDGKIGNKRQLILEMRIWSTNWPYGEENGVEF
                     YGTEGRMVLAKDRKIEIYGERDRFRDDIKLDDLPQMPPSHQADFVEAIRTGKKPTADV
                     IEGHHSVALTHLA"
BASE COUNT          239 a          288 c          309 g          206 t
ORIGIN      
        1 gctgtggggg acggggcgga ggccttgccc aggcatttgc cgagatttcc gacattgcct
       61 atgtctgcga ccccgaccgt tcccgctgcg agcgcttaca gggggcagtg aaagccaaga
      121 aagctgtcac cgatccgcgg caggtcctcg atgaccgatc cgtcgatgcc gtggtcatcg
      181 caacgcccga ccactggcac gcacccttgg ccattttggt ctgtgaggta ggtaaacacg
      241 tttacgtcga aaagcccttg agccataact tccgcgagag tcagctcctt gtcgaggctg
      301 cccggcgggc cagggtcgtc gtacagcacg gcacgcaggc gcgatccagc cctctcatta
      361 acaaagccat tcaactgttg cacaccggcg tgattggaga cgtcctggta gccaaggcgt
      421 ggaatgtgca acgccgtgtc aacattgggc acgagcagcc ctcagaaccg ccgcccggtg
      481 tggattacga catgtgggta ggacctgccg agtttgtgcc cttccaaaag aatcgtttcc
      541 actacacatg gcactggtgg tacaacttcg gcacgggaga catcggcaac gatggtgtgc
      601 atgagcttga ttatggccga tggggattgg gggtttcggg tttgccgaat catgtcatgg
      661 cagaaggggg aaagtacttc ttcgacgatg accaggagtt tcccgacacc gtgacctgcg
      721 ccttcgagtg gacaggcgac ggaaaaattg gaaacaaacg gcaattgatt ctcgaaatgc
      781 gtatctggtc caccaattgg ccgtacggcg aggaaaatgg cgtcgaattt tacggcaccg
      841 agggccgaat ggtacttgcc aaggacagga agatagaaat ctacggagaa cgtgaccgct
      901 tccgcgatga catcaaactg gatgacctcc cgcagatgcc gcccagtcat caagccgatt
      961 ttgtcgaggc gatccgcaca ggaaaaaagc ccacagcgga tgtcattgaa gggcaccatt
     1021 ccgttgccct aacccatctg gc
//
LOCUS       DAOPTR010001256         1038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_113213_length_1038_cov_2.428281, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001256 DAOPTR010000000
VERSION     DAOPTR010001256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113213_length_1038_cov_2.428281"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PKI05_17185"
     CDS             <1..412
                     /locus_tag="PKI05_17185"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOA53991.1"
                     /translation="EASVPFEELDRAAEDILSRCNRNLGNLTTKLLGEALADENLVAK
                     AVFRKAIRTLGWAVAQMITLLAPSAVVIGGGVALLGEKLFFEPLRQEVERYVFPVLIG
                     RYSISSATLGEEVMIHGATLLARNAFASAPDFAA"
     gene            736..>1038
                     /locus_tag="PKI05_17190"
     CDS             736..>1038
                     /locus_tag="PKI05_17190"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOA53992.1"
                     /translation="MTWLDAVIFCGYLLAVFAIGIWFARQQESNEDYFLGGRRMNWIA
                     VGISLFATAFSSLSFVALPREAAFADYHFLLTLLMIPLLITPILYWIFVPTFLRLGV"
BASE COUNT          202 a          274 c          277 g          285 t
ORIGIN      
        1 tgaggcctcg gttccctttg aagagttgga tcgagcggcg gaagacatcc tttctcgctg
       61 caatcgcaac ttagggaact tgacaaccaa attgcttggt gaggccttgg ctgatgagaa
      121 cttggtggct aaagccgtat tccgcaaggc gatccgcacc cttggatggg ccgttgccca
      181 gatgatcacg ctgcttgcac cctcggcggt tgtcataggt ggtggtgtcg cgctcctggg
      241 tgaaaaatta tttttcgaac ccttgcggca ggaggtggag cgttacgttt ttcccgtgct
      301 cattggtcga tactcaattt cttccgcgac tcttggcgag gaagtgatga tccacggggc
      361 caccttactg gccaggaatg ccttcgcatc ggctcctgat tttgcagcgt aatacgtcgc
      421 gcggcacgtt aatcgccgta ggtgagacaa gtcacaaagg gcgaggcgcg gttcgttcct
      481 tttggcgaca cgagttggcg ctgccgtact taaggtctat ccgaataagt ccccctctcc
      541 ctccgggaga gggctggagt gagggtatgc gaacgccgga atcgattgag aaaaccggca
      601 gcgattcgag ccaccctcat cctccatccc tctcccgccg aacgcgtgag gggagattat
      661 gggttagggt cttaactatt tcattgaccg atctccacaa cgctgaataa ccctcagggg
      721 aatgttctcg aatccatgac atggcttgat gcagttatct tttgcggata cttactggct
      781 gttttcgcga taggcatttg gttcgcccgg caacaagaat cgaatgaaga ttactttctt
      841 ggcggccgtc ggatgaactg gatcgccgtt ggtatctccc ttttcgctac cgcgtttagc
      901 tccctgtcat ttgtagcctt accccgggaa gccgcctttg cggactatca tttccttttg
      961 acgttattga tgatccctct tctcatcacc ccaattctgt attggatatt tgtacccacc
     1021 ttccttcgtt taggtgtt
//
LOCUS       DAOPTR010001257         1031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_114346_length_1031_cov_3.426230, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001257 DAOPTR010000000
VERSION     DAOPTR010001257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114346_length_1031_cov_3.426230"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            305..487
                     /locus_tag="PKI05_17195"
     CDS             305..487
                     /locus_tag="PKI05_17195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53993.1"
                     /translation="MGKGLCIFGMVGAGLLLLVFGLDLALGMPFGRISMIMDLGFIVA
                     AAGLFAASFMTLREIP"
     gene            complement(527..>1031)
                     /locus_tag="PKI05_17200"
     CDS             complement(527..>1031)
                     /locus_tag="PKI05_17200"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOA53994.1"
                     /translation="TKIAAIGPGTATALEERYLKPELVPNEYRAEALAEELNRLPARG
                     NILLIRASRGRQVLPEMLSAAGWQVSQVVAYRSVDVTSANPEVIEQLEEGRIDWTTVT
                     SSGIAQSLVRLFGDRLKNTKLASISPVTTKTLHDLGFSPCVEAREYTMAGLVSAILDY
                     ESPNVER"
BASE COUNT          205 a          264 c          298 g          264 t
ORIGIN      
        1 tatctcggtt ggatgagggg tgggataaaa cgctagaaag tcgtaattgc cgttgcactg
       61 gtagtttgcc ggtgccatac tatggtgttt tggtttgttg cggccgtttg gcgtggggaa
      121 aatggcttgg tccgccgagg taaagctatc cggtggaagg gaattgagtc gttgcttccg
      181 cttgaccttg ggtgacacag catcgcgaca agagaggcga gaaagggtga gcggttcgtc
      241 agagtcacct tatttctatc ggggtaacac gcagcagggt atggcacctt gaggatcgac
      301 atcaatgggt aaaggcctct gcatcttcgg tatggttggt gcgggacttc tcctgctcgt
      361 tttcggactc gacctggccc tgggaatgcc gtttgggcgc atcagcatga tcatggacct
      421 tggctttatc gttgcagcgg cgggcctttt tgcagcgagc tttatgacgt tgcgggagat
      481 cccctagcta aattcgcagg tcacctcctc ttcgcttttc tccaaatcag cgctccacgt
      541 taggcgactc gtaatcgaga atcgctgaaa caagtccggc catcgtatat tcgcgggctt
      601 caacacaagg agaaaacccc agatcgtgaa gcgtttttgt ggtgacgggg ctaatgcttg
      661 ccaacttggt gttcttgagc cgatcgccga acaaacggac cagcgattgg gcgattccgg
      721 aacttgtcac ggtcgtccag tcgatgcgac cttcttctag ttgttcaatg acctccgggt
      781 tagccgaggt cacatccacg cttcggtagg ccaccacttg agaaacctgc cagccggccg
      841 cactgagcat ttctggcaat acctgtcttc cccgacttgc cctaataaga aggatgttcc
      901 cccgcgccgg caatcgattc agttcttccg caagggcctc cgcgcgatac tcatttggaa
      961 cgagttcggg tttgaggtaa cgctcttcaa gggctgtggc cgtcccaggt ccaatcgcag
     1021 ctattttggt c
//
LOCUS       DAOPTR010001258         1031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_114361_length_1031_cov_3.072746, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001258 DAOPTR010000000
VERSION     DAOPTR010001258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114361_length_1031_cov_3.072746"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..815
                     /locus_tag="PKI05_17205"
     CDS             33..815
                     /locus_tag="PKI05_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOA53995.1"
                     /translation="MDIATVIGIVGGFLLIVVSIAIAPGASAKAFIDYPSIVMVIGGA
                     IAALLTSMPLKDVLKIVGVVKKALFNKSEDVTAIIQLIVSLAETARRDGLLALENRLT
                     EISDPFIVLGVQMAVDGTRPEVMEDVLRTEMEAVSARHRTGKLIFDQIGKFAPAFGMI
                     GTLVGLIIMLGNMSDPNAIGPGMAVALLTTLYGAIVANVVALPIAEKLGYLSQQEQIR
                     MEIVVRGIMAIQAGENPRVIEQKLRTFVPPKLREQKEDKKAA"
     gene            855..>1031
                     /locus_tag="PKI05_17210"
     CDS             855..>1031
                     /locus_tag="PKI05_17210"
                     /inference="COORDINATES: protein motif:HMM:NF025057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HOA53996.1"
                     /translation="MAGLKEEPSGGGGAPEWVLTYGDMMSLLLTFFIMLAALSEVKKK
                     DEFEMLAEALRRRFG"
BASE COUNT          244 a          242 c          309 g          236 t
ORIGIN      
        1 ccgatgacga tcaatcggag aaaagttgag atatggatat tgcgacagtc atcgggatcg
       61 tcggaggatt cctcctaatt gtggtctcca tcgccatcgc gccaggggcc tcggccaagg
      121 cgtttattga ttatccgtcg atcgtgatgg tgatcggagg cgctatcgct gcgctcttga
      181 cgtccatgcc tcttaaagac gttttgaaga tcgtgggcgt tgtgaaaaag gcgctcttca
      241 acaagagcga ggacgtcacg gcgatcattc aactcatagt ttcattggcg gagacggccc
      301 gtcgcgatgg actcctcgcg ctggaaaacc gtctcacaga aatatcagat ccgttcatcg
      361 tcctgggagt gcaaatggcc gtcgacggca ctcgacccga ggttatggaa gatgtgctcc
      421 gcacggagat ggaagctgta tcagccaggc atcgcacggg aaagctcatc tttgatcaga
      481 ttgggaagtt tgccccggcc tttggaatga ttgggacttt ggtgggcttg atcatcatgc
      541 ttggtaacat gagcgatccg aatgccattg gtcccgggat ggctgtggcg cttttgacaa
      601 ccctttacgg tgccattgtg gccaacgtgg tggccctacc tatcgcggag aaattggggt
      661 acctatcaca gcaagaacaa atccgtatgg aaatcgttgt ccgtgggatc atggccattc
      721 aggccgggga gaatcctcgt gtgatcgagc aaaagcttcg gacgtttgtt ccaccaaagt
      781 tgcgagagca gaaagaagac aagaaggcag cgtagcccgg tgggaaatgt ttctcccagc
      841 gagagagtca cgaaatggct ggcttgaaag aagaaccgtc aggcggtggt ggggcaccgg
      901 aatgggtcct cacatacggg gatatgatgt ctctcttgct gacgtttttc atcatgctcg
      961 cagcacttag cgaggtcaaa aaaaaggacg agtttgaaat gctcgccgag gcgctacgaa
     1021 ggcgattcgg a
//
LOCUS       DAOPTR010001259         1030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_114586_length_1030_cov_2.043077, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001259 DAOPTR010000000
VERSION     DAOPTR010001259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114586_length_1030_cov_2.043077"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(412..>1030)
                     /locus_tag="PKI05_17215"
     CDS             complement(412..>1030)
                     /locus_tag="PKI05_17215"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOA53997.1"
                     /translation="FQIEQAKLELAATELDLEATVLKAPFDGQVLAVPVVAGQAVTPN
                     THIVTLAQVEPLMVRTSIAQNALRHIAPGLAALVTLEAFPGQTWEGHVVRVGNQAVQT
                     PNGIGVELLIELDGSTNPDNSTQTIVPGLTAKTEILVPRKQGVMDIANWFIAALFITM
                     LVMTYAISVIMTPKVEHIPDDSMGGRGGRMGGWGGRGFGGGFGRF"
BASE COUNT          220 a          287 c          268 g          255 t
ORIGIN      
        1 ccggacgatt acggtcaatc cccgcgcgtg cgggggagac cctgccgcgc aacggtaact
       61 attgcatcta tagaaaaatc atcacattaa tccattctga agtgctcgaa atgccaacat
      121 aaagatagtt ccacgtccgg cccgcttctc ctgctcagag gtcagtattt ccgcatcccg
      181 gcacaaatga acatttccgg ttggtgtgat gtcatagccc tgggttcgtc gctttggaac
      241 tgggttactc gcatggtctc catccaatcc tagctgtctg ttgcttttgt ggagcgagca
      301 accgcatgtc gtttggatct tgcgtggagc ggtattctga caaaaacgcc tcccgttgtc
      361 tggcttggaa ggcgcctttt gtcccacgag aaacggcaac gtattcgagt cttagaatct
      421 gccgaaacca ccgccaaaac cgcgaccgcc ccagcctccc atgcgtcctc ctcggccgcc
      481 catgctgtca tcaggaatat gctcgacttt cggggtcatt atgacgctga tcgcgtatgt
      541 cattacaagc atcgttatga atagagcggc tatgaaccag ttcgcgatat ccataacccc
      601 ctgtttacga ggcaccaaga tttcagtttt tgcggtgagg ccgggtacga tcgtttgagt
      661 tgagttatcc ggatttgtgg atccgtccag ttctatcagg agttccactc cgatcccatt
      721 tggtgtctgc acagcttgat tgcctactcg aaccacgtga ccttcccagg tctgcccggg
      781 aaaagcttcc aaagtgacga gcgccgccaa tccgggggcg atgtgtctga gtgcgttctg
      841 agcgatcgaa gtgcgaacca tgagaggctc gacctgggcc aaggtcacga tgtgtgtgtt
      901 tggtgtcacc gcttggccgg cgactactgg cacagccaga acttgaccgt caaagggagc
      961 cttgagaacg gtggcctcca agtctagctc cgtagcagcc aattccagct tagcttgctc
     1021 gatttgaaac
//
LOCUS       DAOPTR010001260         1027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_115018_length_1027_cov_3.698560, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001260 DAOPTR010000000
VERSION     DAOPTR010001260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115018_length_1027_cov_3.698560"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..229)
                     /locus_tag="PKI05_17220"
     CDS             complement(11..229)
                     /locus_tag="PKI05_17220"
                     /inference="COORDINATES: protein motif:HMM:NF020917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HOA53998.1"
                     /translation="MAEVTITVRPRGPYLVQGNVRIVDADRNEYPVDPSKPVALCRCG
                     ASQKRPFCDGAHKSCGFEGDDRATPPGA"
     gene            539..>1027
                     /locus_tag="PKI05_17225"
     CDS             539..>1027
                     /locus_tag="PKI05_17225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA53999.1"
                     /translation="MVPDSQERAPWITYRPELKVLDCTIRDGGLVNDHHFSDEFVKGV
                     YQTCVEAGIDYMEIGYKASKKVFQRDRFGAWKFCDEDDVRRIVGDNPTSLKLAVMMDA
                     GKSDWKTDLLPKDRSVVDMVRVAFYVHQTVEACEMIKAAHEKGYETSANLMAVSQATE
                     VEI"
BASE COUNT          253 a          258 c          291 g          225 t
ORIGIN      
        1 gcctcgggtg ttacgcgcca ggaggtgtcg cccgatcgtc accctcgaat ccgcacgact
       61 tatgggcacc gtcgcaaaac ggtcttttct gcgaggcccc acagcgacac agggcaacgg
      121 gcttggaagg atcaacagga tattcgtttc ggtcggcatc cacgatacga acatttcctt
      181 gcacaagata agggccgcgg ggccgaaccg tgatcgtcac ttccgccatt gacagggtac
      241 ctcccgaatt gacgctttgt atgggtcatt ccgaataatc cagccgaagg gtgtggccct
      301 cagccgatcc tcaagtctat gagggcggtc aaggtcccac gacgaagaca cgcctacttt
      361 tgcagttcga acacccaaaa acgtcacctt gtacacccca ggcgcatgag ttttggtaag
      421 atatagtttc cttggcgggt ctaggattcg gtgaaaagcc gggtggcaag tgtcctttcc
      481 cacaagatga ctccgtttgt gacagtgagc gattgacgac agaaaggaac cgaaagatat
      541 ggtacccgac tctcaggaac gcgcgccctg gattacctat cgtccggagt taaaggtgct
      601 agattgcacg attcgcgatg ggggacttgt caacgaccat catttctcgg acgaatttgt
      661 gaaaggggtg tatcaaacgt gcgtggaagc gggcatcgac tacatggaga ttggttacaa
      721 agcatcgaaa aaagtcttcc agcgcgaccg tttcggggcg tggaaattct gcgatgagga
      781 tgatgtccgg cgaattgtcg gtgacaatcc cacgtcatta aaactcgccg tcatgatgga
      841 tgccggtaaa tcggactgga aaacggatct gctgccaaag gatcgaagcg ttgtggacat
      901 ggtccgggtg gctttctatg tccaccaaac cgtcgaagct tgcgagatga tcaaggctgc
      961 ccacgaaaaa ggttatgaga cgtcggctaa tttgatggcg gtctcacaag ccactgaggt
     1021 cgaaatt
//
LOCUS       DAOPTR010001261         1025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_115392_length_1025_cov_4.009278, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001261 DAOPTR010000000
VERSION     DAOPTR010001261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115392_length_1025_cov_4.009278"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PKI05_17230"
     CDS             <1..582
                     /locus_tag="PKI05_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA54000.1"
                     /translation="SLYQAGNTYSVESEYLDGSGAVTSRDTRSYVVNGPTVFDGYSVV
                     EIQSSINKSTGATQTIKDYYSLGSAEVLKYGFSSTSTFSGSSFTDTTHFVPPEREPHT
                     LQKNVPFAQSFSASGVTVDQTITYLGTESITVPAGTFSACKLQNVSTTNGSTSRVTVW
                     VIADGRYQGLNAKMVDETNVITKQSSKLTFNGL"
BASE COUNT          238 a          305 c          256 g          226 t
ORIGIN      
        1 tcgctttatc aggccggaaa tacctattcg gttgagtccg agtaccttga tggcagtggt
       61 gcggtgacga gcagagacac ccgatcctat gttgtgaacg gcccaaccgt tttcgatgga
      121 tattcagtgg tagagattca gtcctctata aataagtcga ccggcgccac gcagacgatt
      181 aaagattact actcccttgg tagcgcagaa gtcctcaaat acggcttctc tagcacgtcc
      241 acttttagtg gcagcagctt tactgacaca acccacttcg ttccacctga acgtgagcca
      301 cacacacttc aaaagaacgt tccgtttgcc cagtcattct cggcctccgg tgttactgtc
      361 gaccagacta ttacatattt agggaccgaa tccatcacgg ttcctgctgg caccttctcg
      421 gcgtgcaaac tccagaatgt gtccacgacg aatggctcca cttcaagagt aaccgtttgg
      481 gtcatcgccg acggtcgcta tcaagggctt aacgccaaga tggtggacga aacaaatgtc
      541 attactaaac aatcttccaa actgactttt aacggcctgt aaattccctg agatcgtggg
      601 gcctcgttgt ataactctgg ctcaagcaaa gtggccccac cccagacgga tttgagcagc
      661 cagtcgcgcg cccaatgact tgaagcgcag cttgctcgcc tcgaccaggc tacgccggtg
      721 gtagccacgc catttcatcc agaaccgcca tcccaggaag tggggcgcag ccagtatttc
      781 attacggccg tggacaaatc cgtcttgtct ttccagggcg tcctctggtg ttgttgcgtg
      841 taaggcgcgg ggaacaatgc acgcgtcgtt cgctcgccgg agaggcgcgc tccggtcagg
      901 ctaacggccg gtcgatggaa agccacccga agagcagccc ccccaaaaac ctcaccccgc
      961 cccgaaccca aacaattccg gattgctttc gcggtagacc cgcagccaca tcagcgttgc
     1021 gggga
//
LOCUS       DAOPTR010001262         1025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_115486_length_1025_cov_2.125773, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001262 DAOPTR010000000
VERSION     DAOPTR010001262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115486_length_1025_cov_2.125773"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PKI05_17235"
     CDS             complement(<1..380)
                     /locus_tag="PKI05_17235"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOA54001.1"
                     /translation="MKELVVISGKGGTGKTSLTAALAVLSSPCVVTDCDVDAPDLHLV
                     LQPQTIQQQPFVGGRKACIITSTCRKCGKCVELCRFGAIRHKKGDAECEEGGFLIDAL
                     ACEGCGVCVDHCPERAIELRPYVNG"
     gene            complement(352..>1025)
                     /locus_tag="PKI05_17240"
     CDS             complement(352..>1025)
                     /locus_tag="PKI05_17240"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOA54002.1"
                     /translation="CTQCGRCSKACRFGAIISLPQRTVVFPELCHACGGCQLACPAGA
                     ISEKGRRVGTVRAGYAGTIQFLGGELDIGQPMSPPIIRALKETPLGADLVFLDAPPGT
                     SCPVVETLRGADMALLVTEPTPFGLYDLQLAIRLVRALSIPAAVIVNRVVKDGEPFLQ
                     WRQRLKQLPVPVWAEIPESRAIAEAYSSGTIIAQNVPEFARELQPVMHKLKASIDEGT
                     CRHQW"
BASE COUNT          250 a          309 c          254 g          212 t
ORIGIN      
        1 ccattgacat aaggtcgtag ctcgatggcc cgctccggac aatgatcgac acacacgcca
       61 cagccttcac aagccagggc atcaataagg aaaccgccct cttcgcattc cgcatcacct
      121 tttttgtgac ggatggcgcc gaatcggcaa agctccacac atttaccgca tttacggcaa
      181 gttgaagtga tgatacaagc cttacgtcca cccacgaatg gctgctgttg gatcgtctgt
      241 ggctgcaaga ccagatgaag atcaggtgca tccacgtcac agtcggtcac gacacatggg
      301 ctactcaaga ccgccagcgc cgccgtgagc gaagtcttgc ctgttccgcc tttaccactg
      361 atgacgacaa gttccttcat cgattgatgc tttcaatttg tgcataacag gttgtaactc
      421 tcttgcaaat tcaggaacat tttgggcaat gattgtgccg ctcgaatagg cctcggctat
      481 cgcccgactc tccgggatct cagcccacac gggtactggc aattgtttca atcgttgacg
      541 ccattgaaga aatggctcac catctttgac aacgcgattg actatgacgg ccgctgggat
      601 actcaatgcc ctcaccaaac gaatcgcgag ttgaaggtca taaaggccaa agggtgtcgg
      661 ctccgtcacc aaaagagcca tatcagctcc gcgtaacgtc tccactaccg gacaactcgt
      721 ccctggtgga gcatcaagaa aaaccagatc cgcccccaag ggtgtttcct tcaaggcgcg
      781 aataatgggg ggactcattg gctggccaat gtcgagttca ccaccgagaa actgaatggt
      841 cccggcatac cccgcccgaa cggtgccgac ccgccggccc ttttcgctaa tcgccccggc
      901 agggcaagcc aattggcacc cgccgcaggc atgacacaat tcgggaaaga caacggtcct
      961 ctgcggtagg ctgatgatcg ctccaaagcg acaggcctta ctacagcggc cacactgggt
     1021 gcagc
//
LOCUS       DAOPTR010001263         1023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_115852_length_1023_cov_2.098140, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001263 DAOPTR010000000
VERSION     DAOPTR010001263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115852_length_1023_cov_2.098140"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="PKI05_17245"
     CDS             <1..914
                     /locus_tag="PKI05_17245"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOA54003.1"
                     /translation="PDMPGDTAWSDFGTPAWIQELKAAGLPVDSHGRWCREAGGGVPR
                     DWLYVRMARQLLLQHQPNLIMIHLVEPDHVQHRTGPRSDDAYWCASYSDDRIRDLVEA
                     IASSPRAKSTYLVIVSDHGFFPFEKVIQPNVLLRQAGLISVNDGKPVPEKAWCMSQGG
                     AAGIYIFDTQQRDQLMEQLRQAFQSVEGIQAVLTGKEVEALGQKTGQEDPRCPDIWLA
                     AEKGYSFSETSTGDEVVTTRSGLGGTHGYLPDQPDMWAMCVIWGPGIKPGTRLGKVQI
                     VDIAPTIATLLGVALPDADGHALVNPN"
BASE COUNT          228 a          279 c          295 g          221 t
ORIGIN      
        1 ctccggacat gcccggcgac acggcgtggt ccgacttcgg tacaccggcc tggattcaag
       61 aactaaaggc cgcaggcttg ccggtcgata gtcatgggcg gtggtgtcgc gaggccggtg
      121 gcggtgttcc ccgcgattgg ttgtacgtgc ggatggcacg ccagcttctg ttacagcacc
      181 agccaaatct catcatgatt caccttgtcg aaccggacca tgtccagcac cgcactgggc
      241 cccgctccga tgacgcatat tggtgtgcca gttacagcga cgatcgcatc cgcgacttgg
      301 tggaggctat cgcgtcttcg ccgcgggcaa aaagcacgta tctggtaatc gtttcggatc
      361 acggtttttt cccattcgaa aaagttattc aacccaacgt ccttttgcgg caagctggat
      421 taatctccgt caacgatggc aagccagtgc ccgaaaaggc ctggtgtatg tcgcaagggg
      481 gagccgccgg catctacatt ttcgataccc aacagcggga ccagctcatg gaacagcttc
      541 gacaagcgtt ccaatctgtg gaagggattc aggcggttct taccggcaaa gaagtcgaag
      601 ctctgggaca aaaaacaggc caggaggatc ctcggtgtcc agacatctgg ctggcggcgg
      661 aaaaaggtta ctcttttagc gagacgagca ccggcgacga ggttgtgaca actcggtcgg
      721 gtcttggggg aacacacggc tacttgcccg accagcccga catgtgggcc atgtgtgtca
      781 tttggggccc aggaatcaag ccgggcaccc gtttagggaa ggtccaaatc gttgacatcg
      841 ctcccacgat tgccaccctt ttgggggtag cattgcccga cgcagatgga catgccctcg
      901 tcaaccccaa ttgaggaacc aggaagatca acccgcttac ggtgggttcg agaaggcgtt
      961 ttccatttcg tctgggcgtt ggcttgaaaa atcccgaatc attattatga ttatcataaa
     1021 atg
//
LOCUS       DAOPTR010001264         1022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_115957_length_1022_cov_3.065150, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001264 DAOPTR010000000
VERSION     DAOPTR010001264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115957_length_1022_cov_3.065150"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(315..>1022)
                     /locus_tag="PKI05_17250"
     CDS             complement(315..>1022)
                     /locus_tag="PKI05_17250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA54004.1"
                     /translation="HVTDIDPHRPGLEIFSIHERPSHPFGANLRDAATGEIIWGLAAR
                     DVSRGLALDIDPRHPGLECWAVGPGLRGLFNCRGEQIANRAPASCNMGVWWDGDLLRE
                     VLDRTQIQKWIPEREACDVLFDAARYGCTWNNGSKANPTLCADIFGDWREEVIWRSAD
                     NRSLHIFTTPLPSEHRLVTLMHDPVYRLGIAWQNVGYNQPAHPGFYLGHGMTPPRRAK
                     IVTPKALGQSPDRPQTD"
BASE COUNT          210 a          285 c          308 g          219 t
ORIGIN      
        1 attgtcaaag atgggtggag cgaagcgagc tttggtccgg tccaaaccca gggactgcgt
       61 ttagaggttc aacttcggcc aaactggtct ggtggcatct tggagtggca ggtaattgag
      121 gcaaagtgac cgggcggggg ttggggacga gcctcaggtc tcgacggctg gcaatacgtg
      181 ctgaatgccg gatctgcgct cactaggatc gcctctctcg ccgttttgtt aggaagggct
      241 accggaaatc gtggcccgcg ctggccggtg ccatgccata atcagatatg acacgccgtg
      301 tctgagttag gatttcaatc tgtttgcggc cggtcaggtg attggccaag cgccttgggt
      361 gtcacgattt ttgctcgccg gggtggtgtc ataccgtggc ccagatagaa cccagggtgc
      421 gctggttgat tgtagcccac gttttgccac gcgattccca gccgataaac aggatcgtgc
      481 atcaatgtta ccaaacgatg ctcgcttggc aggggtgttg tgaaaatgtg aagactccgg
      541 ttatcagcgg agcgccaaat gacctcttca cgccaatcgc caaagatgtc ggcacacaga
      601 gtgggatttg ctttggagcc attattccag gtgcacccat atcgggccgc gtcgaacaag
      661 acatcgcatg cttcgcgctc gggaatccac ttctgaatct gggttcggtc aagcacttcg
      721 cggagcaggt ccccatccca ccagacgccc atattgcagg acgccggcgc gcggttggcg
      781 atctgttccc cccgacagtt gaaaagccct ctcaagccgg gccccactgc ccaacactcc
      841 aaaccgggat gacggggatc aatgtccagg gccaagccgc gactaacatc tcgggctgca
      901 agcccccata taatctcccc cgttgccgcg tctcggaggt tggccccaaa gggatgtgac
      961 ggacgctcgt gaatggaaaa gatttccagc cctggccgat gcgggtcgat gtccgtcacg
     1021 tg
//
LOCUS       DAOPTR010001265         1016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_117016_length_1016_cov_3.130073, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001265 DAOPTR010000000
VERSION     DAOPTR010001265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117016_length_1016_cov_3.130073"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..639)
                     /gene="pncA"
                     /locus_tag="PKI05_17255"
     CDS             complement(16..639)
                     /gene="pncA"
                     /locus_tag="PKI05_17255"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HOA54005.1"
                     /translation="MDALIVVDVQNDFCPGGALPVPDGDLVVPVINRLMESFPLVVAT
                     QDWHPANHKSFAANHPGRQPGDVIELAGVLQILWPIHCVQGTEGAEFHPDLNRAGIHV
                     VFQKGTDPEVDSYSGFFDNARKHDTGLHEFLQRRGVARLFVCGLATDYCVKFTCLDGR
                     SLGYEVFLVLDASRGVELHTGDVAKAIDEMKAAGVIPIESTEIAMVC"
     gene            complement(738..>1016)
                     /locus_tag="PKI05_17260"
     CDS             complement(738..>1016)
                     /locus_tag="PKI05_17260"
                     /inference="COORDINATES: protein motif:HMM:NF040269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA54006.1"
                     /translation="TQPFGREILPPVFSWHELRSLYEAILGASFSPRRLKSFLVKVGL
                     LKPAESAALQEKPAGKQAKGGLFQFDEAIYQELTKTGFSPFLCGKRCD"
BASE COUNT          256 a          304 c          235 g          221 t
ORIGIN      
        1 cccactcaat tagcctcagc agaccatagc aatttcggta ctctcaatag gtattacgcc
       61 ggcggctttc atctcgtcga tcgccttggc cacatctcca gtgtgaagct caacacctct
      121 tgatgcatcc agcaccaaaa agacctcata acccagcgat ctgccatcca agcacgtgaa
      181 tttcacacaa tagtcggtgg ctaagccaca gacaaaaaga cgagcgacac cccgacgctg
      241 cagaaattcg tgcaaacctg tatcatgctt gcgggcattg tcaaaaaaac cactgtaact
      301 gtccacttcc gggtccgttc ctttttggaa gacaacgtgt atgcctgccc gattcaaatc
      361 gggatgaaac tcagcccctt ctgtcccttg gacgcaatgt ataggccaaa gaatttggag
      421 aaccccggca agctcgatca cgtctcccgg ctgccgtccc ggatgattag ccgcaaagct
      481 cttgtgattc gcgggatgcc agtcctgggt ggcgacaacc aatgggaagc tctccataag
      541 gcggttgatc accggaacaa ctaaatcacc atcgggcacc ggtaaagcgc ctccagggca
      601 gaagtcgttc tgaacgtcaa cgacgatcag ggcatccatt gtcttaaccc tcgcttaata
      661 tgatcggccg cccctgctaa gcgaacgcga gcgtcggcta attcgcgtaa tcccgcattc
      721 gtctcccatc tccatattca gtcacatctc ttcccacaaa ggaacggcga aaatccggtc
      781 ttggtcaatt cttgatagat ggcctcatcg aactggaaca agccaccctt cgcttgcttt
      841 cccgcaggtt tctcttgcag tgccgcgctt tcagcaggct tcaaaagccc gaccttcacc
      901 aggaaggatt tgagacgccg cggcgagaaa ctcgccccga gaatggcttc gtaaagcgat
      961 cggagctcat gccatgaaaa tacgggagga agaatctctc ggccaaacgg ttgtgt
//
LOCUS       DAOPTR010001266         1014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_117339_length_1014_cov_3.861314, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001266 DAOPTR010000000
VERSION     DAOPTR010001266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117339_length_1014_cov_3.861314"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..>1014)
                     /locus_tag="PKI05_17265"
     CDS             complement(121..>1014)
                     /locus_tag="PKI05_17265"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOA54007.1"
                     /translation="CPVSWVTSLSAFRQHLAWLRRHAEVVSLHEIQARLISGNRESRP
                     CVAITFDDGYADNLREAVPLLLEARMPFTFFVTVTNLLQERRFAHDLEQGQSWRLVSP
                     RELRELADSGVEVGSHGLTHADYSLLNIDELVREINDSKHILEDLAGRPVRALAIPFG
                     QPRQMTPEVFQIAYEAGYTCVCSAYGGYNIPGRHRFHIRRFHGDDPLIRLINQVTIDP
                     RLVWQTQRDSVWEAQSERPGSLMRGNEDAIVAPCTLLSQDGEQRPAVGETSARLPTGT
                     APLPEAGAATSPDKVLLGGNL"
BASE COUNT          229 a          296 c          287 g          202 t
ORIGIN      
        1 ccccggcggc ccagggcgaa agactgtgtt gctcggtttc aggcccttga gcgcccccat
       61 cgccaggtgt tgcgacaacg acgtcacctt gccgcaatgt gtcatcacaa gatgctaccg
      121 tcataggttg cctcccagca ggaccttatc cggcgaagtg gccgcgccgg cttccgggag
      181 tggtgcagtt cctgtcggca atctggccga agtctcgccg acagctggcc gctgctcgcc
      241 gtcttgtgat aacaaagtgc atggggcaac aatggcgtct tcgttgccgc gcatgagcga
      301 tccgggcctc tcgctttgtg cctcccaaac ggaatcgcgt tgtgtttgcc agacgagacg
      361 aggatcaatg gtgacctggt tgatcaaccg aatcaaagga tcatcaccgt ggaaccggcg
      421 gatatggaat cggtggcgcc ccggtatgtt gtatccacca taggccgagc aaacgcacgt
      481 gtatcccgct tcgtaggcga tttggaaaac ctcgggcgtc atttgccgcg gctggccgaa
      541 gggaatcgcc agggcccgaa ccggccgtcc cgccaaatct tccaaaatgt gtttgctatc
      601 gttaatttcc cggacaagct cgtcgatgtt caaaagggaa taatcagcat gggtcaatcc
      661 atgggaaccg acctccacac cagaatctgc caactcccga agctcgcgcg ggcttaccaa
      721 acgccagctc tgtccctgct ccaaatcgtg agcaaagcgc cgttcctgga ggagattggt
      781 caccgtcaca aagaacgtaa aaggcattct cgcttcaaga aggaggggta cggcttccct
      841 caggttatcg gcataaccgt cgtcaaatgt gatggccaca cagggacgcg attccctatt
      901 gccagaaatc aaacgggctt gaatttcatg gagagaaacg acttccgcat gacggcgaag
      961 ccatgccagg tgctgccgaa aggccgatag tgaggtcacc caagacacgg ggca
//
LOCUS       DAOPTR010001267         1013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_117544_length_1013_cov_2.797495, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001267 DAOPTR010000000
VERSION     DAOPTR010001267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117544_length_1013_cov_2.797495"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PKI05_17270"
     CDS             <1..216
                     /locus_tag="PKI05_17270"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655260.1"
                     /note="catalyzes the decarboxylation of diaminopimelate
                     into lysine; pyridoxal phosphate(PLP)-binding; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOA54008.1"
                     /translation="GGVVVTRRLPQASVGDYLVIECAGAYGFVMGSNYNAKPLCAEVL
                     IDGGRPQLIRRRQSYRELWEGESIPQG"
     gene            complement(346..657)
                     /locus_tag="PKI05_17275"
     CDS             complement(346..657)
                     /locus_tag="PKI05_17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA54009.1"
                     /translation="MTRKKYTIRVVTRGADGEIRIRDYDSPEDLLKRHTQVGIDDCCT
                     DLSVRGLPVVKGLIGPIPEAPGIVRYESPEVFETLTKEWSMTKITRRRTRTQRPDLNV
                     K"
BASE COUNT          201 a          293 c          291 g          228 t
ORIGIN      
        1 ggcggtgtgg tggtcacgcg gcggcttccc caagcatcgg tgggcgatta cctcgttatc
       61 gaatgtgccg gggcctacgg atttgtcatg ggttcgaatt ataacgccaa acccctttgt
      121 gcggaggtgc tgatcgacgg gggacgtccg caactgatcc gcagacgcca aagttatcgt
      181 gagctgtggg agggagagtc catcccccaa ggttgagcgc ttgctcctcg gaacgagcca
      241 tttacttcta cgaaaccggg aagtttgctc ccaaagacat cctcaaatga agacacccgg
      301 cacgaggccg ggtgctccat cccgaacgtt cgcaacaacc cagcattatt tcacattgag
      361 gtctggtcgc tgcgtccgcg tacggcggcg tgtgattttg gtcatggacc attctttcgt
      421 cagcgtctcg aacacctccg gggattcata gcggacaatc ccgggcgctt ccggaatcgg
      481 gccgataagg cccttgacga caggaagacc acggacgctc aggtcggtac aacagtcgtc
      541 gatgccaacc tgggtatgcc gtttcagcag gtcttcgggg ctgtcgtagt cgcgaatgcg
      601 gatttcgcca tcggcacccc gggttacgac gcgaatggtg tattttttcc tcgtcatggt
      661 ctcctcctcg cctcttaagg gctccttatc cctatccatc ggacctaccg gagtggaact
      721 ccatgctggt cacaagcccc tgtcctgccc ggaatgacct gtttgaactg tcccgctgat
      781 agaacagtca tcagacgtac cagcggcggt ggtatgcgcc gtcagacatt tgcggcggat
      841 tatctacgaa gcgggatcga actaatcgac actctaagaa ttaccgcgag gaacgctctt
      901 ggcctgccgc gtcgtgacaa tcatcttaaa gcctatccca gaatctcccc tctcccggtc
      961 agcgggagag gggtcggggg tgagggtggc tcgaatcgct gccggttttc tca
//
LOCUS       DAOPTR010001268         1002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermogutta sp. isolate AMR_MDS_1439
            NODE_119596_length_1002_cov_2.515312, whole genome shotgun
            sequence.
ACCESSION   DAOPTR010001268 DAOPTR010000000
VERSION     DAOPTR010001268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854644
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermogutta sp. (wastewater metagenome)
  ORGANISM  Thermogutta sp.
            Bacteria; Planctomycetota; Planctomycetia; Pirellulales;
            Thermoguttaceae; Thermogutta.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:09:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,456
            CDSs (total)                      :: 3,423
            Genes (coding)                    :: 3,408
            CDSs (with protein)               :: 3,408
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Thermogutta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119596_length_1002_cov_2.515312"
                     /isolate="AMR_MDS_1439"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1962930"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1002)
                     /locus_tag="PKI05_17280"
     CDS             complement(<1..>1002)
                     /locus_tag="PKI05_17280"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOA54010.1"
                     /translation="KKKISDWVFERESVQASAESLARGFISTGDPLFDKQYTERIQAV
                     TAEQIREVARKYFRPEWLNEVIIAPLGRSPATVKADQTSDETAARLVRLNNGLRVVVK
                     RQPRLPIVTMQIAILGGNLVDTPETAGRSSLLAMMLDKGTERLTAEQVAEMFDDLDAR
                     VSFTPGRNTLLVGLSILKDSFTPAAELLAECLLKPRFSEDEFAKVKVLALDAIAQRTA
                     NPQAEAQELFADLLPEESPYHVVMGGKKETVERITINDLKNLHAQILNPEQMVVAVYG
                     DCDPERAVELVDRLFGGLRPPANPPKISFDRSNKIARIISGHKQIQKPTSVIVMGYP"
BASE COUNT          218 a          285 c          257 g          242 t
ORIGIN      
        1 cgggatagcc catcacaata acactcgtgg gcttttggat ttgcttgtgc ccggagatga
       61 tgcgagcaat cttattactg cgatcgaaac taattttcgg gggattggca ggcggacgga
      121 gaccaccgaa gagccggtcc accagttcca cagccctctc tggatcacaa tcgccgtaga
      181 cggcaaccac catctgttcc ggattgagga tctgtgcgtg aaggttcttg aggtcgttta
      241 tggttattcg ttctacagtt tcctttttgc cgcccatgac aacgtggtaa ggcgattcct
      301 ccggcaagag gtcggcaaac aactcctgag cctctgcctg cggattggcc gtccgctgag
      361 cgatcgcgtc aagggctagg actttcactt tcgcgaattc atcctcggaa aagcggggct
      421 ttagtagaca ttctgccaaa agctctgccg ccggtgtgaa agaatccttg aggatcgaca
      481 accccactag caacgtattg cgaccgggcg taaaactgac ccgcgcatca agatcatcaa
      541 acatttccgc gacctgctct gccgtcaacc gctctgtgcc tttgtcgagc atcatggcta
      601 gcagcgaaga tcgaccggca gtttccggcg tgtccacgag atttccccca aggatggcaa
      661 tttgcatggt cacaatcgga agccgtggct gccgcttaac aactacccgc aggccgttgt
      721 tgagacggac caacctcgcg gccgtttcgt cgcttgtttg atccgccttc accgttgcgg
      781 gagatcgacc caacggggca atgatgacct cgtttagcca ttctgggcga aaatactttc
      841 gcgcaacttc cctaatctgc tccgccgtaa ccgcttggat tcgctctgta tattgcttgt
      901 caaaaagggg atcgccggta ctgatgaagc cacgggccaa actttcggca ctggcctgga
      961 cactttcgcg ttcaaaaacc caatcggaaa tcttcttctt tt
//
